LOCUS       DBFIFM010000001        81490 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.001, whole genome shotgun sequence.
ACCESSION   DBFIFM010000001 DBFIFM010000000
VERSION     DBFIFM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 81490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.001"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            59..1396
                     /gene="frlA"
                     /locus_tag="N7C74_000001"
     CDS             59..1396
                     /gene="frlA"
                     /locus_tag="N7C74_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HGU2429965.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPATTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTPLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            1417..2439
                     /gene="frlB"
                     /locus_tag="N7C74_000002"
     CDS             1417..2439
                     /gene="frlB"
                     /locus_tag="N7C74_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HGU2429966.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDARCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPHAEIGKIKNDLKQLPNALGYLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAAISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            2490..3317
                     /gene="frlC"
                     /locus_tag="N7C74_000003"
     CDS             2490..3317
                     /gene="frlC"
                     /locus_tag="N7C74_000003"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HGU2429967.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDE"
     gene            3317..4102
                     /gene="frlD"
                     /locus_tag="N7C74_000004"
                     /pseudo
     CDS             3317..4102
                     /gene="frlD"
                     /locus_tag="N7C74_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
     gene            4202..4933
                     /gene="frlR"
                     /locus_tag="N7C74_000005"
                     /gene_synonym="yhfR"
     CDS             4202..4933
                     /gene="frlR"
                     /locus_tag="N7C74_000005"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HGU2429968.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERISAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(5064..6068)
                     /gene="trpS"
                     /locus_tag="N7C74_000006"
     CDS             complement(5064..6068)
                     /gene="trpS"
                     /locus_tag="N7C74_000006"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HGU2429969.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(6061..6819)
                     /gene="gph"
                     /locus_tag="N7C74_000007"
     CDS             complement(6061..6819)
                     /gene="gph"
                     /locus_tag="N7C74_000007"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HGU2429970.1"
                     /translation="MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(6812..7489)
                     /gene="rpe"
                     /locus_tag="N7C74_000008"
     CDS             complement(6812..7489)
                     /gene="rpe"
                     /locus_tag="N7C74_000008"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HGU2429971.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(7507..8343)
                     /gene="dam"
                     /locus_tag="N7C74_000009"
     CDS             complement(7507..8343)
                     /gene="dam"
                     /locus_tag="N7C74_000009"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HGU2429972.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(8450..9736)
                     /gene="damX"
                     /locus_tag="N7C74_000010"
     CDS             complement(8450..9736)
                     /gene="damX"
                     /locus_tag="N7C74_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HGU2429973.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(9828..10916)
                     /gene="aroB"
                     /locus_tag="N7C74_000011"
     CDS             complement(9828..10916)
                     /gene="aroB"
                     /locus_tag="N7C74_000011"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HGU2429974.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGTFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(10973..11494)
                     /gene="aroK"
                     /locus_tag="N7C74_000012"
     CDS             complement(10973..11494)
                     /gene="aroK"
                     /locus_tag="N7C74_000012"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HGU2429975.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(11895..13133)
                     /gene="hofQ"
                     /locus_tag="N7C74_000013"
     CDS             complement(11895..13133)
                     /gene="hofQ"
                     /locus_tag="N7C74_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HGU2429976.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(13045..13449)
                     /gene="hofP"
                     /locus_tag="N7C74_000014"
     CDS             complement(13045..13449)
                     /gene="hofP"
                     /locus_tag="N7C74_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HGU2429977.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTLDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(13439..13879)
                     /gene="hofO"
                     /locus_tag="N7C74_000015"
     CDS             complement(13439..13879)
                     /gene="hofO"
                     /locus_tag="N7C74_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HGU2429978.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQHWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVFWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(13863..14402)
                     /gene="hofN"
                     /locus_tag="N7C74_000016"
     CDS             complement(13863..14402)
                     /gene="hofN"
                     /locus_tag="N7C74_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HGU2429979.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(14386..15180)
                     /gene="hofM"
                     /locus_tag="N7C74_000017"
     CDS             complement(14386..15180)
                     /gene="hofM"
                     /locus_tag="N7C74_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HGU2429980.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGWRLGRRTDEPAN"
     gene            15300..17852
                     /gene="mrcA"
                     /locus_tag="N7C74_000018"
     CDS             15300..17852
                     /gene="mrcA"
                     /locus_tag="N7C74_000018"
                     /EC_number="2.4.99.28"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HGU2429981.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(18017..18577)
                     /gene="nudE"
                     /locus_tag="N7C74_000019"
     CDS             complement(18017..18577)
                     /gene="nudE"
                     /locus_tag="N7C74_000019"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HGU2429982.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            18897..21032
                     /gene="igaA"
                     /locus_tag="N7C74_000020"
     CDS             18897..21032
                     /gene="igaA"
                     /locus_tag="N7C74_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HGU2429983.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHLVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            21097..21765
                     /gene="yrfG"
                     /locus_tag="N7C74_000021"
     CDS             21097..21765
                     /gene="yrfG"
                     /locus_tag="N7C74_000021"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HGU2429984.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            21776..22177
                     /gene="hslR"
                     /locus_tag="N7C74_000022"
     CDS             21776..22177
                     /gene="hslR"
                     /locus_tag="N7C74_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HGU2429985.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            22202..23080
                     /gene="hslO"
                     /locus_tag="N7C74_000023"
     CDS             22202..23080
                     /gene="hslO"
                     /locus_tag="N7C74_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HGU2429986.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(23143..24890)
                     /gene="yhgE"
                     /locus_tag="N7C74_000024"
                     /pseudo
     CDS             complement(23143..24890)
                     /gene="yhgE"
                     /locus_tag="N7C74_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
     gene            25269..26891
                     /gene="pckA"
                     /locus_tag="N7C74_000025"
     CDS             25269..26891
                     /gene="pckA"
                     /locus_tag="N7C74_000025"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HGU2429987.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVSEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(26967..28319)
                     /gene="envZ"
                     /locus_tag="N7C74_000026"
     CDS             complement(26967..28319)
                     /gene="envZ"
                     /locus_tag="N7C74_000026"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HGU2429988.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQSTTKEG
                     "
     gene            complement(28316..29035)
                     /gene="ompR"
                     /locus_tag="N7C74_000027"
     CDS             complement(28316..29035)
                     /gene="ompR"
                     /locus_tag="N7C74_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HGU2429989.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            29263..29739
                     /gene="greB"
                     /locus_tag="N7C74_000028"
     CDS             29263..29739
                     /gene="greB"
                     /locus_tag="N7C74_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HGU2429990.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            29836..32157
                     /gene="yhgF"
                     /locus_tag="N7C74_000029"
     CDS             29836..32157
                     /gene="yhgF"
                     /locus_tag="N7C74_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HGU2429991.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLGYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIITDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAENEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            32595..32822
                     /gene="feoA"
                     /locus_tag="N7C74_000030"
     CDS             32595..32822
                     /gene="feoA"
                     /locus_tag="N7C74_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HGU2429992.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            32839..35160
                     /gene="feoB"
                     /locus_tag="N7C74_000031"
     CDS             32839..35160
                     /gene="feoB"
                     /locus_tag="N7C74_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HGU2429993.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            35160..35396
                     /gene="feoC"
                     /locus_tag="N7C74_000032"
     CDS             35160..35396
                     /gene="feoC"
                     /locus_tag="N7C74_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HGU2429994.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            35599..36477
                     /gene="rpnA"
                     /locus_tag="N7C74_000033"
     CDS             35599..36477
                     /gene="rpnA"
                     /locus_tag="N7C74_000033"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HGU2429995.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYPDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDIT
                     VMPDDEIMQHRRMTLLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            complement(36504..37274)
                     /gene="bioH"
                     /locus_tag="N7C74_000034"
     CDS             complement(36504..37274)
                     /gene="bioH"
                     /locus_tag="N7C74_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312282.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HGU2429996.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLAEMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRLPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFRHVLVALKQRV"
     gene            37312..37995
                     /gene="gntX"
                     /locus_tag="N7C74_000035"
     CDS             37312..37995
                     /gene="gntX"
                     /locus_tag="N7C74_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HGU2429997.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAITRTRAT
                     ATQHFLSARLRRRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            38054..38629
                     /gene="nfuA"
                     /locus_tag="N7C74_000036"
     CDS             38054..38629
                     /gene="nfuA"
                     /locus_tag="N7C74_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HGU2429998.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            38990..40306
                     /gene="gntT"
                     /locus_tag="N7C74_000037"
     CDS             38990..40306
                     /gene="gntT"
                     /locus_tag="N7C74_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HGU2429999.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(40351..42435)
                     /gene="malQ"
                     /locus_tag="N7C74_000038"
     CDS             complement(40351..42435)
                     /gene="malQ"
                     /locus_tag="N7C74_000038"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HGU2430000.1"
                     /translation="MESKRLDNAALAAGISPHYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHITGGKAFNLPT
                     KLPEGYHTLTLTQEDQRGHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWQAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(42445..44838)
                     /gene="malP"
                     /locus_tag="N7C74_000039"
     CDS             complement(42445..44838)
                     /gene="malP"
                     /locus_tag="N7C74_000039"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HGU2430001.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            45450..48155
                     /gene="malT"
                     /locus_tag="N7C74_000040"
     CDS             45450..48155
                     /gene="malT"
                     /locus_tag="N7C74_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HGU2430002.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            48222..48500
                     /locus_tag="N7C74_000041"
     CDS             48222..48500
                     /locus_tag="N7C74_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HGU2430003.1"
                     /translation="MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACRE
                     AQDILLDQRLFILDDEQYDAFLAALDAPITAERQAKINALMNRKSPWE"
     gene            48491..48976
                     /locus_tag="N7C74_000042"
     CDS             48491..48976
                     /locus_tag="N7C74_000042"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU2430004.1"
                     /translation="MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRV
                     YVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAPESLPVVVLGRLAIDQAWAGKGL
                     GVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
                     E"
     gene            complement(49026..50054)
                     /gene="rtcA"
                     /locus_tag="N7C74_000043"
     CDS             complement(49026..50054)
                     /gene="rtcA"
                     /locus_tag="N7C74_000043"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HGU2430005.1"
                     /translation="MKRMIALDGAQGEGGGQIMRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAATEICGATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAASFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGQFTVAHPSCHLLTNIAVVERFLPVRFTLAETDGVTR
                     VMITKLTD"
     gene            complement(50058..51284)
                     /gene="rtcB"
                     /locus_tag="N7C74_000044"
     CDS             complement(50058..51284)
                     /gene="rtcB"
                     /locus_tag="N7C74_000044"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HGU2430006.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDVMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKMFSVEDQIRATVHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            51472..53070
                     /gene="rtcR"
                     /locus_tag="N7C74_000045"
     CDS             51472..53070
                     /gene="rtcR"
                     /locus_tag="N7C74_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HGU2430007.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSLYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVADGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFASSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(53052..53810)
                     /locus_tag="N7C74_000046"
     CDS             complement(53052..53810)
                     /locus_tag="N7C74_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HGU2430008.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDTPPPVSVMQVLTDHHIQLELC"
     gene            complement(53827..54657)
                     /gene="glpG"
                     /locus_tag="N7C74_000047"
     CDS             complement(53827..54657)
                     /gene="glpG"
                     /locus_tag="N7C74_000047"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HGU2430009.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(54702..55028)
                     /gene="glpE"
                     /locus_tag="N7C74_000048"
     CDS             complement(54702..55028)
                     /gene="glpE"
                     /locus_tag="N7C74_000048"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HGU2430010.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRNNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            55218..56723
                     /gene="glpD"
                     /locus_tag="N7C74_000049"
     CDS             55218..56723
                     /gene="glpD"
                     /locus_tag="N7C74_000049"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HGU2430011.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAVSDLGEDFGHEFYETE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(56938..57540)
                     /locus_tag="N7C74_000050"
     CDS             complement(56938..57540)
                     /locus_tag="N7C74_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2430012.1"
                     /translation="MMNIIRLYIYPLFLGFVIVPLLVWPTVVALAACVITLSFLGEIL
                     FSIPLMHRRISLLQLQLWLSAEYALFFCAMVGVGWQFARRTPPELKSRLHCWLVFAPV
                     YFWLLLWNIIFYVAPAQIALLENMRSFFLTVVWLPLNFSPFWSQQWIDFVGPISAQLG
                     FALGYYCQWRSKNRSQIRKWSEWGTCLSFVTLGMMAWALR"
     gene            complement(57540..58532)
                     /locus_tag="N7C74_000051"
     CDS             complement(57540..58532)
                     /locus_tag="N7C74_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HGU2430013.1"
                     /translation="MNKFTGVLLLGTALLAGCVDREGYYNSVREEESHGLTSLRGQPA
                     LRYSDDWSRWPRVYGATALYPLYASAYYKLVPEPKDKDRTSLAWQAYGLQQTRTAEAY
                     DSLIKGSATVIFVAQPSEGQKKRAEEAGVKLKYTAFAREAFVFIVDINNPVNSLSEHQ
                     VKDIFSGKTSRWNKVGGSDEHIKVWQRPEDSGSQTIMKGLVMQDTPMLPAKKSTVIDL
                     MGGLITEVADYQNTPSSIGYTFHYYVTRMNDNMLKMRKQIKLLAINGVAPTEENIRNG
                     TYPYIVDAYMVTRENPTPETQKFVDWFISQQGQQLVEDVGYVPLYEASPESSGQ"
     gene            complement(58558..60066)
                     /locus_tag="N7C74_000052"
     CDS             complement(58558..60066)
                     /locus_tag="N7C74_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HGU2430014.1"
                     /translation="MMPNRKWILTSLIMTFFGIPILAQFLAAVVAMLGVGLTGIIEVC
                     NILITPTIYLLLNVFMLTLGAIIIFFSGRVWAGDSAPENREIAVWRQCFFLLPALLTL
                     VGWIITLHLADYQFRQMGAGWLANLMLPWLGVFLVSLVGGEYWWMVIIPVGAHISFSL
                     GYAWPTRYPLSGTSGLRCRNLLLFLLLLLGIVAGYQAHLYKQQNPGVGVRENIDIRAW
                     RPDKLNNRLTPLRGKPQIQFRQNWPRIDGATAAYPIYASAFYALSVIPEDFHVWEYLE
                     NSRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKKVRMISP
                     QETEVASVMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIRLLAINGITPTAENI
                     RNGKYAYIVDAFMVTRENTTSETQKLVEWFLTPQGQSLVEDVGYVPLYLTME"
     gene            complement(60191..62638)
                     /gene="glgP"
                     /locus_tag="N7C74_000053"
     CDS             complement(60191..62638)
                     /gene="glgP"
                     /locus_tag="N7C74_000053"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HGU2430015.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADLDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(62657..64090)
                     /gene="glgA"
                     /locus_tag="N7C74_000054"
     CDS             complement(62657..64090)
                     /gene="glgA"
                     /locus_tag="N7C74_000054"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HGU2430016.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(64090..65385)
                     /gene="glgC"
                     /locus_tag="N7C74_000055"
     CDS             complement(64090..65385)
                     /gene="glgC"
                     /locus_tag="N7C74_000055"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HGU2430017.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(65403..67376)
                     /gene="glgX"
                     /locus_tag="N7C74_000056"
     CDS             complement(65403..67376)
                     /gene="glgX"
                     /locus_tag="N7C74_000056"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HGU2430018.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGVGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(67373..69559)
                     /gene="glgB"
                     /locus_tag="N7C74_000057"
     CDS             complement(67373..69559)
                     /gene="glgB"
                     /locus_tag="N7C74_000057"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HGU2430019.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPLNEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(69832..70935)
                     /gene="asd"
                     /locus_tag="N7C74_000058"
     CDS             complement(69832..70935)
                     /gene="asd"
                     /locus_tag="N7C74_000058"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGU2430020.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            71127..71720
                     /gene="yhgN"
                     /locus_tag="N7C74_000059"
     CDS             71127..71720
                     /gene="yhgN"
                     /locus_tag="N7C74_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HGU2430021.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(71766..73061)
                     /locus_tag="N7C74_000060"
     CDS             complement(71766..73061)
                     /locus_tag="N7C74_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HGU2430022.1"
                     /translation="MDADSSNSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASKYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(72968..75092)
                     /locus_tag="N7C74_000061"
                     /pseudo
     CDS             complement(72968..75092)
                     /locus_tag="N7C74_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
     gene            complement(75232..76572)
                     /gene="gntU"
                     /locus_tag="N7C74_000062"
     CDS             complement(75232..76572)
                     /gene="gntU"
                     /locus_tag="N7C74_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HGU2430023.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(76576..77103)
                     /gene="gntK"
                     /locus_tag="N7C74_000063"
     CDS             complement(76576..77103)
                     /gene="gntK"
                     /locus_tag="N7C74_000063"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HGU2430024.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(77242..78237)
                     /gene="gntR"
                     /locus_tag="N7C74_000064"
     CDS             complement(77242..78237)
                     /gene="gntR"
                     /locus_tag="N7C74_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HGU2430025.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(78461..79156)
                     /gene="yhhW"
                     /locus_tag="N7C74_000065"
     CDS             complement(78461..79156)
                     /gene="yhhW"
                     /locus_tag="N7C74_000065"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HGU2430026.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASISDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(79279..80316)
                     /gene="yhhX"
                     /locus_tag="N7C74_000066"
     CDS             complement(79279..80316)
                     /gene="yhhX"
                     /locus_tag="N7C74_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HGU2430027.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLNEVLNDPDVKLVVVCTHADSHFEYAKRAMEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            80650..81138
                     /gene="yhhY"
                     /locus_tag="N7C74_000067"
     CDS             80650..81138
                     /gene="yhhY"
                     /locus_tag="N7C74_000067"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGU2430028.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDARWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYFMAR
                     VK"
     gene            81349..>81490
                     /locus_tag="N7C74_000068"
     CDS             81349..>81490
                     /locus_tag="N7C74_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2430029.1"
                     /translation="MGTAAISHLRYDLNKYALSLRKTATLASTFFIESPLVRFEYLQE
                     IEN"
BASE COUNT        19677 a        22125 c        20438 g        19250 t
ORIGIN      
        1 cttttaaatg actgcaaact ctcccctaca acgtgttgga caagaaaaag gtatcgctat
       61 gggaagccag gaactccaac gcaagctcgg attttgggcc gttcttgcaa tcgccgtcgg
      121 gacaaccgtc ggctccggta tttttgtatc tgtgggtgaa gtggcaaaag cggcgggcac
      181 gccgtggctg acggtgctcg catttgtcat cggcgggtta attgtgatcc cgcaaatgtg
      241 cgtatatgcg gaactatcca ccgcttatcc ggaaaatggc gcagattatg tttatctgaa
      301 aaatgccgga agccgaccgc tggctttcct ctccggctgg gccagcttct gggccaacga
      361 tgcgccgtca ttgtcgatta tggcgctggc gattgtcagc aatcttggct ttttaacgcc
      421 tatcgatccg ttgctcggta aatttatcgc cgccggatta attatcgcct ttatgctgct
      481 gcacctgcgc tccgttgaag gcggcgcagc gtttcagacg ctaattacca tcgccaaaat
      541 tatcccgttc actatcgtca ttggtcttgg gatcttctgg tttaaagcgg agaattttgc
      601 cgcccctgct accactgcga ttggcgcaac gggcagcttt atggcgctgc tggcggggat
      661 ctctgccacc agttggtcgt ataccggcat ggcctctatc tgttatatga ccggcgaaat
      721 taaaaacccc ggcaaaacca tgccacgagc gctgattggt tcctgtctgc tggttctggt
      781 gctctacacc ctgctggcgc tggtgatttc cggcctgatg cccttcgaca aactcgccaa
      841 ttctgaaacg ccgatttccg acgccctgac ctggatcccc gcactcggca gcaccgctgg
      901 gatctttgtt gccatcacgg cgatgatcgt cattcttggt tcgctttcca gctgcgtgat
      961 gtaccagccg cggctggaat acgcgatggc gaaagacaac ctgttcttca aatgcttcgg
     1021 ccatgtgcat ccgaaataca acacgccgga tgtctccatc attttgcaag gggcgctggg
     1081 gatcttcttc atcttcgttt ccgatctcac cagcctgctg ggctatttca ccctggtaat
     1141 gtgtttcaaa aataccctca ccttcggctc catcatctgg tgtcgtaaac gcgacgatta
     1201 caaaccgctg tggcgtactc cggctttcgg gctgatgacc cccctcgcca ttgcgtcaag
     1261 cctcattctg gtcgcctcaa cctttgtctg ggcaccgatt cccggcctta tctgcgccgt
     1321 catcgttatt gccactggtc tgcctgctta cgccttctgg gcgaagcgta gccgccagct
     1381 caacgctttg tcgtaatgtt atctggagaa aataaaatgt tggatattga taaaagcact
     1441 gtggactttc tggtcaccga aaatatggtt caggaagtgg aaaaagttct cagccatgac
     1501 gttccgctgg tgcacaccat cgtggaagag atggtgaagc gcgacatcga tcgtatttat
     1561 ttcgttgcct gcggatcgcc actcaacgcg gcgcaaacgg cgaaacatct ggcagatcgc
     1621 ttttccgatc ttcaggttta cgccatttcc ggctgggagt tctgcgataa caccccatat
     1681 cgcctcgacg ctcgttgcgc ggtgattggc gtttctgact acggtaaaac cgaagaggta
     1741 atcaaagcgc tggagctggg ccgggcctgc ggcgcactca ctgcggcgtt caccaaacgc
     1801 gcggatagcc cgattacctc ggcggcggaa tttagcattg attatcaggc cgactgtatc
     1861 tgggaaattc atctgctgct ctgctacagc gtggtgctgg agatgatcac ccgcctcgcg
     1921 ccgcacgcgg aaatcggcaa gatcaaaaac gatctcaaac agctgccgaa tgcgctcggt
     1981 tatctggtac gcacttggga agaaaaaggc cgccagcttg gtgaactggc cagccagtgg
     2041 ccgatgattt acacggttgc tgcaggcccg ctgcgcccgc tgggttacaa agaaggcatc
     2101 gtgacgctga tggaatttac ctggacgcac ggctgtgtga ttgagagcgg tgagttccgt
     2161 cacggcccgc tggagattgt cgaaccgggc gttccgttcc tgttcctgct cggcaatgat
     2221 gaaagtcgcc acaccaccga acgcgccatt aactttgtta aacagcgtac tgacaacgtg
     2281 atcgtcatcg attacgccgc aatttcgcaa ggactgcacc cgtggctggc accgttcctg
     2341 atgttcgtgc caatggagtg gctctgctac tacctgtcta tttacaaaga tcacaacccg
     2401 gatgaacgcc gctattacgg tggtctggtg gaatattaat cctctctccc ggcccggcaa
     2461 cgggccggat ttacgcaagg agttacccga tgaaaacagg tatgtttacc tgcggccacc
     2521 agcggctgcc tattgaacat gcattccgtg atgcaagcga gctgggttac gatggcatcg
     2581 aaatttgggg cggccgcccg cacgcgttcg cgccggactt aaaagcgggc ggcatcaaac
     2641 aaatcaaggc gctggcgcag acgtatcaga tgccgattat cggttacacg ccagaaacca
     2701 acggctatcc gtataacatg atgctgggcg atgaacatat gcgtcgtgaa agcctggaca
     2761 tgatcaagct ggcgatggat atggcaaaag agatgaacgc gggttatacg ctgatttctg
     2821 cggcccacgc gggctatctc acgccgccta acgttatctg ggggcggctg gcagagaact
     2881 taagcgaact gtgtgagtac gcggaaaaca tcggcatgga tctgatcctc gaaccgttaa
     2941 cgccgtatga atcgaacgtc gtgtgtaatg ctaatgatgt gcttcatgcg ctggcgctgg
     3001 tgccttcgcc gcgcctgttc agcatggtcg acatttgcgc gccgtatgtc caggcggaac
     3061 cggtgatgag ttatttcgac aaactgggcg ataaattacg tcatctgcat attgtcgaca
     3121 gcgacggggc cagcgacacg cattacattc ccggcgaagg caaaatgccg ctgcgggaac
     3181 tgatgcgcga tattattgat cggggctatg agggatactg cacagtggaa ctggtaacca
     3241 tgtatatgaa cgagcccaga ctttacgccc gccaggcgct ggaacgcttt cgcgcgctgc
     3301 tgccggagga tgagtgatga aaaccctggc gacaatcggc gataactgcg tcgatatcta
     3361 cccgcaactg aataaagcgt tttctggcgg taatgcggtg aacgtggcgg tgtactgcac
     3421 tcgctacggt ataaagccgg gatgcattac ctgggttggt gacgatgact acggcacaaa
     3481 gcttaagcag gatctcgcca gcatgggcgt cgatatcagc catgtccata cgaaacacgg
     3541 cgttaccgca caaactcagg tggaactgca cgacaatgat cgcgtttttg gcgactacac
     3601 cgaaggcgtg atggccgact ttgccctgag tgaagaggat tacgcctagc tggcgcagta
     3661 tgacattgtg cacgcggcaa tctggggaca tgcggaagac gcattcccac agctgcacgc
     3721 tgcgggcaaa cttaccgctt tcgacgtctc cgacaagtgg gacagcccgc tctggcagac
     3781 actggtgccg catctcgatt ttgcctttgc ctccgcaccg caagaagacg aagcgctgcg
     3841 tctgaagatg aaagcgattg ttgcccgtgg cgcaggcgtg gtgattgtca cgttgggtga
     3901 aaacggcagt attgcctggg atggcgtgca gttctggcgt caggctcctg aacaggtgac
     3961 ggttatcgac accatgggtg ccggagattc gttcattgcc ggattccttt gcggctggtc
     4021 tgcggggatg acattaccgc aggcgatggc gcagggaacg gcgtgcgcgg cacaaactat
     4081 tcagtaccac ggtgcctggt aggcataacg ttggcgtgag catcttcacg ccaacgtgct
     4141 gttacttgcc ggaaaacgac cctataatcc gtgtaattca ttctttattt cagggtcgat
     4201 tatgtcagct acggaccgct actctcatca actcctctac gctaccgtcc gccagcggct
     4261 gctggatgat atcgcgcagg gggtttacca ggccgggcaa cagatcccta ccgaaaatga
     4321 gctttgtaca caatataacg tcagccgcat taccattcgc aaagccatca gcgacttagt
     4381 ggcagacggc gtactgatcc gctggcaggg caaaggcacc tttgtacaaa gccagaaggt
     4441 cgaaaacgcc ctgctgaccg tcagtggttt taccgatttt ggcgtctcac aaggcaaggc
     4501 gacgaaagag aaagtgatcg agcaagaacg gatcagcgcc gcgccgtttt gcgaaaagct
     4561 caatatcccc ggtaacagcg aagtgttcca tctttgccgg gtgatgtatc tcgataaaga
     4621 gccgctattt attgatagtt cgtggatccc gctgtcgcgt tatcctgact ttgatgagat
     4681 ttacgtcgaa ggaagctcca cctatcagtt atttcaggag cgttttgaca cgcgagtggt
     4741 cagcgacaaa aagaccatcg atatctttgc cgccacccgc ccgcaggcaa aatggctgaa
     4801 atgcgaactg ggcgaaccgt tgtttcgcat cagcaaaatc gcctttgatc agaatgacaa
     4861 accggtgcac gtctccgaac tcttctgccg cgccaaccgc atcaccttaa caattgataa
     4921 taaaagacat taaccgtagg ccggataaga tgcaccagca tcgcatccgg cgatgccggc
     4981 gcactgaatt ttacatcccg cacgttcccc tcaccctaac cctctcccca aaggggcgag
     5041 gggaccgtcc gagcgttttg atattacggc ttcgccacaa aaccaatcgc ttcgtacacc
     5101 gcttttagcg tacgggaagc gtgcgcgctg gctttttctg cgccgtcttt catcacctgt
     5161 tgcaggaagg cttcatcgtt gcggaaacgg tggtaacgtt cctgcaattc agtcagcata
     5221 ccggaaacgg catcagccac ttcgcctttc agatgaccat acatcttgcc ttcgaactgt
     5281 ttttccagtt ccgggatgct ctggcccgtt accgcagaaa ggatatccag caggttggaa
     5341 acgcccgctt tgttctgcac atcgtagcga actaccggcg gctcgtcgga gtcggtgacc
     5401 gcgcgtttga ttttcttcac taccgatttc ggatcttcca gcaggccaat aacgttattg
     5461 cggttatcgt cagacttgga catcttcttg gtcggctcca gcagcgacat tacgcgcgcg
     5521 ccggatttcg gaataaacgg ctccggcacc ttaaagatat cgccgtacag cgcgttgaaa
     5581 cgctgggcga tatcacggct cagttccagg tgctgtttct ggtcttcacc caccggtacc
     5641 agattagttt gatacagcag gatgtccgct gccatcagca ccggatagtc aaacagacca
     5701 gcgttgatgt tctcggcata acgcgcagat ttatctttaa actgcgtcat gcggctcagt
     5761 tcgccgaagt aggtatagca gttcagtgcc cagcctaact gcgcatgttc cggtacgtgg
     5821 gactgaacaa aaatggtgct tttctcagga tcgataccac aagccagata caaggccagc
     5881 gtatccagcg tcgctttacg cagcttctgt gcatcctggc gcacggtgat cgcgtgttgg
     5941 tcaacgatac agtaaatgca atggtagtca tcctgcatgt ttacccactg acgcagcgca
     6001 cccatgtagt taccaatagt caattcacct gagggctgtg cgccactaaa aacgatgggc
     6061 ttagtcattt ttcgattcct gattttcgct atgcggaagc cctaatgcgg gcagaaggtc
     6121 atttatagac tgataaatta catcaggctg gctgagatcg atagcctcgc cgtagttata
     6181 tccgtaggtt aagccaactg atgggcaacc tgccgctttt gctgcctgaa tatcattgcg
     6241 tgagtcgcca acaaacagca tctgttgtgg agcaattccc atccgctcag ccaccagtaa
     6301 cagcgggtcc ggatgcggtt ttttgttttg cacatcatca ccaccaatca ccacgctgaa
     6361 gtatttggcg atatctaagg cttcgagcag cggcgcgacg aacggcgtcg gtttgttggt
     6421 gaccaggcct agcggcaggc ctttagcctg caacgcgccc aacgtatcgg caacgtgcgg
     6481 gaacaaaaac gtcccctctt cggcaacctc gccatagtaa cgatcgaaca gtttacgcag
     6541 aatacgtacc tgttcttctg ccggaatgtc gtcatcaacg ggcggtttac ccattgtttt
     6601 acgcagagtc gcacgttcct gacgcgccca ggtcaatgcg cgctccatca gaacatctgc
     6661 gccgttacca atccaggtaa taacgcgttc ttcacctgcg acgggcaact ccagcgcata
     6721 cagcgccata tctaccgcag cagcaagacc gggagcactg tccaccagcg taccgtcgag
     6781 atcaaaagcg acgccgcgaa tatcttcaaa cttactcatg acttaccttt gccagttcac
     6841 tgcgcatttc atcaatgact tttttgtagt ctggctggtc gaagattgcc gaaccggcaa
     6901 cgaacatatc cgcgcccgcc gcggcgattt cgccaatgtt gttcaccttc acgccaccgt
     6961 ccacttccag acgaatgtca aagccagact cgtcgatacg acgacgtact tcgcgcagtt
     7021 tatccagtgt ttgaggaatg aaagattgac cgccgaaacc agggttgacg gacatcagca
     7081 ggatcacatc cagcttatcc atcacgtaat ccagatagct cagaggtgtc gccgggttaa
     7141 ataccagacc cgctttacag ccattttctt taatcagttg cagcgtgcgg tcaacatgct
     7201 cggaggcttc tggatgaaag gtaatgatgc tggcaccagc ggcagcgaaa tcaggcacaa
     7261 tgcgatcgac cggtttcacc atcaggtgta cgtcgatagg ggcggtaatg ccgtagttac
     7321 gcaaggattt cagcaccatt ggcccaatcg tcagattggg aacgtagtgg ttatccatga
     7381 cgtcaaaatg cacgacatca gcgccagctg ccagggcttt tgcggtatct tcacccaggc
     7441 gggcaaaatc agccgacaga attgaggggg caatcaaata ctgtttcatc cgcttctcct
     7501 tgagaattat tttttcgcgg gtgaaacgac tcctggtttg tacaaagcca gcagttcgtc
     7561 caccttttta cgtgtgccgc cgttgctgct tatactgcgt cgaactttga cgacatgcaa
     7621 ttttgcgcgc tgataccact cacgcgttaa catcgtatcg tgattggaga tcagcacagg
     7681 aatatggcgc tcaaccagac cttcggcgat ctccgccaga tgcgcttgtt gttcaagcgt
     7741 aaaactgttt gtgtgatacg ccgtaaagtt ggcggtcgca gacagcggtg cataaggtgg
     7801 atcgcaatag acgacagatg catcatctgc gcgcgccatg ctatcggcgt aagactcaca
     7861 atagaaaaag gcattctgcg ctttttcagc gaagtgatac aactctgctt ccgggaaata
     7921 gggttttttg tagcggccaa acggcacatt aaactcaccg cgcagattgt aacgacacag
     7981 gccgttgtaa ccgtagcggt tcaaatataa aaacagtacc gcccgacgga acggatcctg
     8041 gcttttgttg aactcttcgc ggaactgata gtaaacctcg gcgcaatttg tttcgggaac
     8101 aaacagctcg cgtgcggcct gtacgtactc atcagtacgc atcttcacaa tgttatagag
     8161 actgatcagg tcgctattga tatcggcaag gatataacga gaaaagtcgg tgttgagaaa
     8221 caccgacccg gcacctacaa aaggctcaac cagacattcg cccttgggca aatgccgttt
     8281 aatatcatca agcaggggat acttgccccc tgcccacttc aaaaaagcgc gatttttctt
     8341 catgctgact aactaattac accttctccg gctgtggaga aagctccgac agcatccagc
     8401 gcttcaggca actcccgcaa cgataaacca ttgcgggaga taaccttgat tacttcagat
     8461 cggcctgtac ctgacgcagc ggtttcgccc acgggttttt ggcctgaaca tctgctggca
     8521 atgtagatac cgcttttttc gcctcttctt tcgaagcgta cacgccagaa accaggacat
     8581 accacggctg accattacgc gtcgtttcat agacaacgta gtttttcaga ttctctttct
     8641 tcgcccaacc gttcaggttg tcgtagttag aggaactgct cagctgcaga gtgtaatggc
     8701 tggacggtgc cgatttcaac gaacccacat tacctgcggt cttccctcca gcggctggtg
     8761 ttgccgtggt ttgcgccggg gatgccgtct gtactggagc cgtagtcgcc gtctcttttg
     8821 gtgctggcgt agaagtcgca gccggtgcct tcgtgctcgc cacaggagca gctggttcag
     8881 tacgcttcgg cgtctgtgct accggcttcg gttccgtttt cacggttgct tgcggttttt
     8941 tcggttcaat caccgcctgc tgacgagctg gacgcgtagt ggccggacgt tcagcagttt
     9001 gcgttttcgc cgtgtcacgc gatgcattgc cattgcgtac tggcgcgaca gttgctggtt
     9061 cagtcggcaa tgtagaattg accgccacat tgttcaactg ttgctgattt tgtggctggg
     9121 tcagcgcatt gttcaggtca ccctgcactt caacacgttg ttgaccatcc gttgccgccg
     9181 gggtttgccc ttgagtcggc gtagaagaga tcggtggcag agaaacatcc tgctgagtat
     9241 tttccgcaga cgtggttccc ggcgctggct gcacgccatt cgcctgatcg gtcgcattgc
     9301 cagcaagatc aatactcttc tcgccagacg cggtttgatc gctggaagtg gtcgaggggg
     9361 cttttagcgc agaaccgata ccgatgatca acagcagtag aaccagaatg ccgacgccca
     9421 tcatcatata ctgacgagaa gcgggtttac tggctgcttt tttgcgctta cgcggacgac
     9481 gctctacgcg ctcttcatcc acggtttcat cttcggattc gtcaatttct tcttcgattt
     9541 ccggttcctc attgcgctct ttttgcgcac gagtcggacg gcgatcgtca gtgtcatcaa
     9601 gttcaatatc atcaaaattg atctgcggtt cgccacgttc agtacgctca gaacgttcag
     9661 aagattgacg agaacgacca gtacgacgat cgctgggatc gggtttcagc tcgtcttctg
     9721 gtttgaattc atccatttaa caccccacta aaaggttaat gcttaccacg ttgcaattaa
     9781 cctgaagcta atagaccgct tgataagcgg cctgaccttt cttgttgtta cgctgattga
     9841 caatcggcaa tggcgttaag aacaagctcg tgcgaaacgc cgctgcgaac ttcactctta
     9901 ccaattgcca acggaagaat taagcgcatc tctcccgcaa ggactttctt gtcacgcagc
     9961 atatgcggta aatacgcctg cgcggacatt tcgcgcggcc cattgaccgg taacccagcc
    10021 cgcgtgagca gagttataat acgctgcgtt tcggcagaac taaactgccc gagacgttcc
    10081 gacgtccgcg ccgccatcac catacccgca gcgaccgctt caccatgtaa ccaattgcca
    10141 taccccattt cagcttcaat ggcatgacca aaggtgtgtc ccagattcag taaagcacgt
    10201 aacccggttt cgcgctcgtc ggcggcgaca acttctgcct tcagttcaca acaacggcga
    10261 atacagtacg ccattgccgg accgtccaga cgcaacaacg catccagatt ctcttccagc
    10321 cagttaaaaa acgtaccgtc aagaataatg ccgtatttga tgacttctgc cagccccgac
    10381 gctaactcac gcgggggaag cgttttcaga cagtcgagat ccaccaccac tgaagcaggc
    10441 tggtagaacg cgccaatcat gtttttaccg aggggatggt tgaccgcagt tttgccgcca
    10501 acggaggaat cgacctgcga cagtaacgtc gtcgggactt gaatgaaacg aacaccgcgc
    10561 tgataactcg ccgccgcgaa gccggtcaga tcgcccacta cgccgccgcc aagcgccacc
    10621 agcgtagtat cgcgaccatg cggcttttgt aacaacgccg taaagacggt atcgagtaca
    10681 gccaggcttt tatactgctc gccgtcagga aggataacgc tatcgacgtt aacacccgcc
    10741 tgttcaagta cgccgcggac cttatcgaga tacagaggag ccagggtttc gttggtgacc
    10801 aacatgacct gctcgcccga tttcagcggt aagaatgaag ctggttcatt aaacaaacca
    10861 gatgcgatgg taattgggta actacgttcc ccgagagtaa cgacaatcct ctccataacg
    10921 cgacatccac cttaattact gtacccgcag acgagtgtat ataaagccag aattagttgc
    10981 tttccagcat gtgaataatc tggtttgcaa ccactttagc gctttgatca tcagtacgaa
    11041 tggtcacgtc ggcaatctct tcatacagcg gattgcgttc attggccaac gcttccagaa
    11101 cttcacgcgg cggtgtttca acgtgcagca acgggcgttt tttatcacgc tgcgtgcgtg
    11161 caagttgctt ttcgatcgtc gtttcaagat aaacgacaac gccacgagcg gaaagacggt
    11221 tacgcgtttc acgggatttc acagagccgc cgccagtagc cagcacaata ccctgtttct
    11281 cggtcaactc attgatgacc ttttcttcgc gatcgcggaa gccttcttcg ccttctaaat
    11341 cgaaaaccca gcccacatca gctccggttc gtttctcaat ctcttgatcg gaatcgtaaa
    11401 attccatatt gagttgttga gctaactggc gcccaatagt gctttttccg gcacccatag
    11461 gcccaaccag aaagatattg cgtttctctg ccattttttc ggtactacta agactattcg
    11521 ttaatgataa acccgcttcg ctcagagagc tccgcaggac atgaactgaa acctcataag
    11581 atattgcgag agtcagactg aaaattatct caatactcca gcgggtttgg caactgaata
    11641 aatcacccat ccctctgcat atctggtcgc tgcaagcgcg ctgccttgct accaccgctc
    11701 tggcgataaa tcaccgggta agattagcgt aaaaaagaca gcaaaatgcc gcctgaatga
    11761 taaatcatca tcatggaagg caggcagcga agagcaccaa ctctcaaatc ggtactcctt
    11821 gtatgctaaa tacctgcgcg cgtcaaatag atgaaacacg ttcagcgtaa aaacattacc
    11881 gcttttacgg ctgtttactc actggaaacc agtcgtggcg tgataaacac cactaactcg
    11941 cgtcgttcat cttcttttcc gtcatgacga aataattgcc cgaaccaggg aatgtcgcca
    12001 agcaacggca cgctatcctg acccgattta tttttacggg taaaaatgcc gcccagcgcc
    12061 aacgtttctc cgcttttgac ctcgacctgc gtttcgatct cctgcttatc aatcgccagc
    12121 acttcgccat cggcctgctg tagcacctgc cccggaacgt tctggctgat gtgtaatttc
    12181 agccggatgc gacctttttg taacaccgtg ggcgtgacct ccatccccag gacggcctct
    12241 ttaaattcca ccgacgtcgc gccactttcc ccgctggaaa cctgatatgg aatttcgctc
    12301 ccctgtttaa tgctggcagg ctgaagatgt gaggccagca gacgcggact ggcgataata
    12361 tccagctgct gtttttgttc gagcgcggaa agctcaagat ccagcaaacg tccgttgatg
    12421 cgcccaatgt taaaaccgac atgcgttgtc gccgtcgcta cggagaggtc gctaccaagc
    12481 gtggtgactt gcccaacgcc accagcgtgt tgcgcatcgg ccagcgtcca tttcacgcct
    12541 aactcacgca aacttttttc attaatggtg acaatatgcg ccgacagctc aacctgcccg
    12601 accggcagat ccatttgcgc tacccactgt tcaagggcgc ttaacgccgt tttgttatct
    12661 cgtagcaaaa ggcgattggt gcgtttatcg acggtcatac tccctttggc actcagtagc
    12721 ttctccccag ctttcgccag ctctccggcg tcggcgtatt gcagagtaat attgcgattt
    12781 tccagcggca gatttgcctg cgcccgcgcc tgctccgctt cctgacgggc gatattgtca
    12841 ttctgccagg caatggaatg cactgagaga atgttgccct cctggcgcgt tatcagtccg
    12901 gcgcttttca ctacagtttg tagtgcctgc ttccagggaa catctgttag atgtaacgac
    12961 accgtaccgc tgacgtctgg cgacaccacc aggttcaact tctcctgttc agccagcgcc
    13021 tgcaacacct gagctaccgg aacgtcatcc accatcagcg tcactttttg cggctttgcc
    13081 gcctgtacgc cgggtatcag catcaacagt agtgcggcta tccattgctt catttgtatc
    13141 tccttgccgt tgccacaacc attgtggcgg ttcgcagttt gtcccggtac ccagcgttag
    13201 tacgtctagc gtcagctgta aaattgtcca gccgttttcc agcacatcgt tttgctgcac
    13261 ccgtcgccat ttcttttgcc cgtcttttat tacaccgatg atgcgctcgc ctcgccctac
    13321 catcccctga tagcgccact ggctaagttc gctaatccgg catagatctt ccggcggttt
    13381 aaaagggtca cgcataccgg ttaaaaggca caatgcaata cctgccaaca accagcgttt
    13441 aaccctcatg cggcgtctcc agttgtaggg tgaacaaaag atcatcacct tccacgctta
    13501 atgaaaaacg gctcacgctg acgttgcgct ctgccagccg tgtaaatgcc gacggcactg
    13561 cttcccacag cgttttcaac gccaactcgc ctccctgcgc ggatggatgc cagaaaacca
    13621 gttgtgcgcc gggcaactga aaatccagtg gcgaaaaggg cagcgttttt tcttcgctga
    13681 agggagtggt gtcaaccagg cgatacagtg tggcccaatg ctgatgatga cttgcccgta
    13741 gtcgaattaa tgcgtcgcgc tcttcatggt gtgtcgatga cagaaaaatg agcgtaacta
    13801 acatcagcaa ccagaatgcc cagcaaaact ggcggaggcg gggtgatgtg gcgaaccacc
    13861 agtcaaagaa catgttcatc gctaaccttc cttgttaact gatactcaaa ttgccagcgt
    13921 ccctgcgcat cctgctgcgt ggccccccgc tgattgagat gaaaagatgc atcctggcgg
    13981 agtgacgttt ctagtgcgtt taacgcggta atgcttgttg taagcccctt gatctccagc
    14041 gttccctgct gccagcttat cgttgtcagc caggcgtgct cgggtaaaag cgccgccagt
    14101 gcttccagcg cagattgcca gtcgcgggta aattgtcgct ggcgctgcct ttgcaaacgc
    14161 tgctcacgaa tttgttggcg ctccagcaaa cgtggtttgg ttatctgtaa gctgcgggcg
    14221 agttgttgtt ccccctgaag caaaacggcg tctacgcgag cttcggcgct gcttgtcaga
    14281 cgcagtatta gcgttatccc gacggccagc agcagaggcg caacgaacat cagcaaccag
    14341 aaacgaagaa aagcggtccg gcgttgctgt cgccagggca aaaaattaat tggcgggttc
    14401 atcagtacgc cttcccaagc gccagcccag cgcgatggca aagtctccac ctggcggcgg
    14461 taacggcggc tgacgaacag aaacggcctc ccaaggatca aatccgcctt cgccacatat
    14521 cgcgacgctt tcgggatcaa cggataacgc tgccgccagc tccttcgcgc tagtcatccc
    14581 caccgccagt ttgcgccccc agcgatagcg cgtcgcccac agccactgtt cgttatcacg
    14641 ccaggccaga cattgctgat gagaaggtaa aaaaggcagg aatcgctgta atgcactggc
    14701 atccggggtg atcgcactaa catgaacacg caacctttct gccaaagtaa gcagcgttgc
    14761 cagctctttg ctttgcgcgg cagtcacgtt ataggcggga ctgagtgagt cttcgctgta
    14821 atcgaagcgc agggagtccg gatccatatc cagctcgcga gccatcgtcc cggacagcca
    14881 ggccgtttgc tcccgctcac cgagggacat cgacgggcgc ggaaatgacc gctgtaatgt
    14941 gcgactggcg ggaaacgcca acatgatgtg atggcgctgc ggcagttcgc gactccacgg
    15001 taacaacgtt ttagccagcc gctgcgcatc aacaatccgc ccatctttga taatgtcgtg
    15061 cgccagcggc aaccgccacc agcgttgcaa aaagcattct tttgcgcccc gcacaatagc
    15121 aaccgctacc gcttcttgct gttgtaaatg caaaccaatt tgccagatct taaatgccat
    15181 tgtgatgatc tccttatcac ccgtcactct gacgggtata tcaatgcgtc tggcttgcct
    15241 ttatactacc gcgcgtttgt ttataaactg cccaaatgaa actaaatggg aaatttccag
    15301 tgaagttcgt aaagtatttt ttgatccttg cagtctgttg cattctgctg ggagcaggct
    15361 cgatttatgg cctataccgc tacatcgagc cacaactgcc ggatgtggcg acattaaaag
    15421 atgttcgcct gcaaattccg atgcagattt acagcgccga tggcgagctg attgctcaat
    15481 acggtgagaa acgtcgtatt ccggttacgt tggatcaaat cccgccggag atggtgaaag
    15541 cctttatcgc gacggaagac agccgcttct acgagcatca cggcgttgac ccggtgggga
    15601 tcttccgtgc ggcaagtgtg gcgctgttct ccggccacgc gtcacaaggg gcaagtacca
    15661 ttacccagca gctggcgaga aacttcttcc tcagtccaga acgcacgctg atgcgtaaga
    15721 ttaaggaagt cttcctcgcg attcgcattg aacagttgct gacgaaagac gagatcctcg
    15781 agctttatct gaacaagatt taccttggtt accgcgccta tggtgtcggt gctgcggcac
    15841 aagtctattt cggaaaaacg gtcgaccaac tgacgctgaa cgaaatggcg gtgatagccg
    15901 ggctgccgaa agcgccttcc accttcaacc cgctctactc gatggatcgt gccgtcgcgc
    15961 ggcgtaacgt cgtgctatcg cggatgctgg atgaaggata tatcacccag caacagttcg
    16021 atcagacacg cactgaggcg attaacgcta actatcacgc gccggagatt gctttttctg
    16081 cgccgtacct gagcgaaatg gtgcgccagg agatgtataa ccgttatggc gaaagtgcct
    16141 atgaagacgg ttatcgcatt tacaccacca tcacccgcaa agtgcagcag gccgcgcagc
    16201 aggcggtacg taataacgtg ctggactacg acatgcgcca cggctatcgc ggcccggcaa
    16261 atgtgctgtg gaaagtgggc gagtcggcgt gggataacaa caagattacc gatacgctga
    16321 aggcgctgcc gacctatggt ccgctgctgc ctgccgcagt caccagcgcc aatcctcagg
    16381 aagcgacggc aatgctggcg gacgggtcga ccgtcgcatt gagtatggaa ggcgttcgct
    16441 gggcgcgtcc ttaccgttcg gatactcagc agggaccgac gccgcgtaaa gtgaccgatg
    16501 ttctgcaaac gggtcagcaa atctgggttc gtcaggttgg cgatgcatgg tggctggcac
    16561 aagtgccgga agtgaactcg gcactggtgt cgatcaatcc gcaaaatggt gccgttatgg
    16621 cgctggtcgg tggctttgat ttcaatcaga gcaagtttaa ccgcgccacc caggcactgc
    16681 gtcaggtggg ttccaacatc aaaccgttcc tctacaccgc ggcgatggat aaaggtctga
    16741 cgctggcaag tatgttgaac gatgtgccaa tttctcgctg ggacgcaggt gccggttctg
    16801 actggcagcc gaaaaactca ccaccgcagt atgccggtcc aattcgctta cgtcaggggc
    16861 tgggtcagtc gaaaaacgtg gtgatggtac gcgcaatgcg ggcgatgggc gtcgactacg
    16921 ctgcagaata tctgcaacgc ttcggcttcc cggcgcaaaa cattgtccac accgaatcgc
    16981 tggcgctggg ttcagcgtcc ttcaccccaa tgcaggtggc gcgcggctac gcggtcatgg
    17041 cgaacggcgg cttcctggtg gacccgtggt ttatcagcaa aattgaaaac gatcagggcg
    17101 gcgtgatttt cgaagcgaaa ccgaaagtag cctgcccgga atgcgatatt ccggtgattt
    17161 acggtgatac gcagaaatcg aacgtgctgg aaaataacga tgttgaagat gtcgctatct
    17221 cccgcgagca gcagaatgtt tctgtaccaa tgccgcagct ggagcaggca aatcaggcgt
    17281 tagtggcgaa gactggcgcg caggagtacg caccgcacgt catcaacact ccgctggcat
    17341 tcctgattaa gagtgctttg aacaccaata tctttggtga gccaggctgg cagggtaccg
    17401 gctggcgtgc aggtcgtgat ttgcagcgtc gcgatatcgg cgggaaaact ggtaccacta
    17461 acagttcgaa agatgcgtgg ttctcgggtt acggaccggg cgttgtgacc tcggtctgga
    17521 ttggctttga tgatcaccgt cgtaatctcg gtcatacaac ggcttccgga gcgattaaag
    17581 atcagatttc aggttacgaa ggcggtgcca agagtgccca gcctgcatgg gacgcttata
    17641 tgaaagccgt tcttgaaggt gtgccggagc agccgctgac gccgccgccg ggtattgtga
    17701 cggtgaatat cgatcgcagc accgggcagt tagctaatgg tggcaacagc cgcgaagagt
    17761 atttcatcga aggtacgcag ccgacacaac aggcggtgca cgaggtggga acgaccatta
    17821 tcgataatgg cgaggcacag gaattgttct gattaaaaag gcgcttcggc gccttttcag
    17881 tttgctgaca aagtgcactt gtttatgccg gatacgggtg aacgcgttat ccggctaaca
    17941 aaatcgtgaa aactcaatat attgcaggaa ccccataagc ctgataagca ttgtgcatca
    18001 ggcaaacttc acgcatttac actcgcccct gccctttcaa ccattcgcgc acgaggaaca
    18061 gcgcgctgac attacgcgct tcgttgaagt cagggtcttc cagcaaatcc atcatatgcg
    18121 ccagcggcca gcgcacctgt ggtagcggct ctggctcatc gccttccagc gattccgggt
    18181 agagatcttg cgctaccacg atattcattt tgctggaaaa gtaagacggt gccatgctga
    18241 gcttcttcaa aaaagtcaga tcgttcgcac caaatccaac ctcttctttt agctcgcggt
    18301 tagcggcttc gaagacgctt tcacccggat caattaatcc tttcgaaaaa cctaattcgt
    18361 aggattcagt tcccactgca tattcgcgga tcaggatcag gtgatcgtcc acaatcggca
    18421 caatcatcac tgcttcccgg ttggttggac gcattcgttc ataaacacgc cgcacgccat
    18481 tgctgaactc tagatccacg ctctcgacgg taaacagtcg ggaacgggct acagtttcaa
    18541 cattcagaat ggtgggtttt tgtaatgatt tgctcatcgt gggatctatg ctgtgaaatc
    18601 agcggttatt gtgcgatatc ggacacgctt tcggcaatgt gaattgcatg ttatttacat
    18661 ttatgtaact taataaataa tcgtcctcaa atcaaattaa aagtcaatag gttgaaataa
    18721 ctccaggaat ttgctgatat tccgccttca gagggtttgc tatgatcagc ggttactgtg
    18781 atgtgcttaa tgatgctcaa gttaaactcc acgcttgccg atagccaacc gcagaatcat
    18841 gtattgtgtc cggtgcgact gaccacgcct gacagactaa gtaagatggg gaaagcatga
    18901 gcaccattgt gattttttta gctgctttgc tggcctgctc actacttgcg ggatggctga
    18961 taaaagtgcg atccagacgg cgtcagctgc cctggaccaa cgccttcgcg gatgcgcaaa
    19021 cgcgtaaact cacacctgaa gaacgtagcg ccgttgaaaa ttatcttgag agcctgacgc
    19081 aggtattaca ggtgcctggc ccaacgggag ccagcgcggc accgatctct ctggcgctga
    19141 atgccgaaag caacaacgtc atgatgctga cacacgctat cacgcgttac ggcatctcta
    19201 ccgacgatcc gaataaatgg cgttactacc tcgattcggt agaagtccac ctgccccctt
    19261 tctgggagca gtacatcaac gatgagaata ccgttgaact gattcatacc gattcgctgc
    19321 cgctggttat ttcactcaac ggtcatacgc tgcaggagta catgcaggaa actcgcggct
    19381 atgccttgca acctgttccg tccacgcagg cgtcgattcg cggggaagag agtgagcaaa
    19441 tcgagctact caatattcgc aaagaaacgc atgaagaata tgcgctgagt cgtccgcgcg
    19501 ggctgcgtga agcgttgctg atcgtcgcct ccttcctgat gttctttttc tgcctgatta
    19561 ccccggatgt atttgttccg tggctggcag gcggcgcgtt actgctgctg ggcgcaggtc
    19621 tgtgggggct attcgccccc ccggcaaaat cctccctgcg ggaaattcat tgtctgcgcg
    19681 gtacaccccg tcgttgggga ttgtttggcg aaaacgatca ggaacagatc aacaatattt
    19741 cactcggtat tatcgacctg gtctatcccg cacactggca gccatacatt gctcaggatc
    19801 tcggtcaaca aaccgatatt gatatctatc tcgaccgcca cctagtgcgt caggggcgat
    19861 atctttcgct gcatgatgaa gtaaaaaact ttccgttaca gcactggctg cgcagtacga
    19921 ttatcgctgc gggctcgctg ctggtgctgt ttatgctgtt attctggatc ccgctggata
    19981 tgccgctgaa attcactctc tcatggatga aaggtgcgca gaccattgaa gccaccagcg
    20041 taaaacaact ggctgacgct ggcgtgcggg taggcgatac attgcgtatt agcggtacgg
    20101 gaatgtgtaa tattcgcact tccggaacct ggagcgcgaa aaccaattca ccttttttac
    20161 cgtttgactg ctcgcagatc atctggaacg acgcccgctc attaccatta ccagaatctg
    20221 aactggtcaa caaagcgacg gcattgactg aagcagttaa tcgccagctg caccctaaac
    20281 cggaagatga atctcgcgtc agcgcctcat tacgttcagc aattcaaaaa tccggcatgg
    20341 tattgcttga tgattttggc gacattgtac tgaagacagc ggatctgtgt tctgccaaag
    20401 atgactgtgt gcgactgaaa aatgcgctgg tcaatctcgg caacagtaaa gactgggacg
    20461 cgctggtaaa acgcgccaac gccgggaagc tcgatggcgt gaatgtgtta ttacgcccgg
    20521 tgagtgcgga atcgctggat aacctggtgg caacctccac cgcgccgttt atcacgcatg
    20581 aaacggcgcg agcggcacaa tcactaaaca gtccggcccc cggcggattc ctgattgtca
    20641 gcgacgaagg cagcgatttt gttgatcagc cctggccttc ggcatcactt tacgactacc
    20701 cgccgcaaga acagtggaac gctttccaga aactggcaca aatgctgatg catacgccgt
    20761 ttaacgccga gggtatcgtc acaaaaatct tcactgacgc caatggtacg cagcatatag
    20821 gccttcatcc gatcccggat cgttccggcc tgtggcgcta tctcagcacc acgctgctgc
    20881 tactgacgat gctgggtagc gccatttata atggcgtaca ggcctggcgg cgttaccagc
    20941 gtcatcgcac tcgcatgatg aagattcagg cctattatga aagctgcctg aacccgcaac
    21001 tgatcacccc ttcagaaagc cttatcgaat aacacgtttg cgcggcaggt tatgctaccc
    21061 tgtcgcgcaa attgcttcac tctggagatt ttcctcatgc atatcaacat tgcctggcag
    21121 gacgtagata ccgttctgct ggatatggac ggcacgttgc tcgacctcgc cttcgataac
    21181 tatttctggc aaaagctggt gcctgaaaca tggggcgcga aaaacggggt tacgccacag
    21241 gaagcgatgg aatatatgcg ccagcaatat cacgacgtac agcatacgct aaactggtac
    21301 tgtcttgatt actggagtga gcaactgggt ctggatatct gtgcgatgac caccgagatg
    21361 ggaccgcgtg ccgtactgcg tgaagatacc attccgtttc ttgaggcact gaaagccagc
    21421 ggtaagcagc gaattttgct caccaatgcg catccgcaca acctggcggt aaaacttgag
    21481 cataccggtc tggacgcaca ccttgattta ttactttcca cccacacatt tggttatccg
    21541 aaagaggatc agcggttatg gcatgcggtg gccgaagcta cgggtctgaa agctgaaaga
    21601 acgctgttta ttgatgacag cgaagcgatt ctcgatgctg ccgcgcaatt tggtattcgt
    21661 tactgcctcg gcgtgactaa tcctgactca gggattgccg agaaacagta tcaacgccat
    21721 ccgtcactga atgactaccg ccgcctgatc ccctcgctaa tgtgaaggag acgccatgaa
    21781 agaaaaacct gctgttgagg ttcgactgga taaatggcta tgggctgccc gtttttataa
    21841 aacccgcgcg ctggcccgtg aaatgattga aggcggtaag gtgcattaca acgggcagcg
    21901 cagcaagccg agcaaaatcg tcgagctgaa tgccacgctc actctgcgcc agggaaatga
    21961 cgaacgcacg gtgattgtaa aggcgattac tgaacagcgt cgccccgcca gcgaggcagc
    22021 cttgctgtat gaagagactg cggaaagtgt agagaaacgc gaaaaaatgg cgctggcacg
    22081 taaacttaat gccttaacca tgccgcaccc ggaccgacgc ccggacaaaa aagagcgccg
    22141 cgacctgtta cgatttaaac acggcgacag tgaataactg tcacctgcaa gagagatgat
    22201 tatgccgcaa catgaccaat tacatcgcta tctgtttgaa aactttgccg tgcgcggcga
    22261 actggtaacc gtttcggaaa ccctgcaaca gatccttgag aatcacgatt atccgcagcc
    22321 cgttaaaaac gtgctggcag aactgctggt tgcgaccagc ctgttaaccg ctacgctgaa
    22381 gtttgatggt gatatcaccg tacagctgca aggcgacggt ccgatgaatc tggcggttat
    22441 taacggtaac aacaaccagc agatgcgcgg tgtggcgcgc gtgcagggcg aaattccaga
    22501 aaatgccgac ctgaaaacgc tggtcggcaa tggttacgtg gtgatcacca ttaccccgag
    22561 cgaaggtgaa cgctatcagg gcgtggtggg tctggaaggt gataccctgg cggcctgcct
    22621 ggaagattac tttatgcgtt ctgaacagct gccgacgcgc ctgtttattc gcaccggcga
    22681 cgtagacggc aaaccggctg caggcggtat gttgttgcag gtaatgcctg cgcaaaatgc
    22741 ccagcaggac gactttgacc acctggcgac gctaaccgaa accatcaaaa ccgaagaact
    22801 gctgacctta ccggcaaacg aagtgttgtg gcgtttgtat cacgaagaag aggtgacggt
    22861 ttacgatccg caggatgtgg agttcaaatg cacctgctcg cgtgaacgtt gcgccgatgc
    22921 gctgaaaacg ctacctgatg aagaagtcga tagcatcctg gcggaagatg gcgaaattga
    22981 catgcattgt gattactgcg gtaaccacta tctgttcaat gcgatggata ttgctgaaat
    23041 ccgcaacaac gcgtctccgg cagatccgca agttcattaa tgagtatgtc cggcagagag
    23101 agctctgccg gattttacga ctaatacacc tgatgcgctt tgtcactcat tcagattcac
    23161 atccagcctt tcaccatcca ctacaagatc gcgccacgct ttaggcttcg ttcccagttt
    23221 tccatccttc gcagctgtga gtaatttttc tttcgttata tcacgcggaa gtaaactcat
    23281 cgtaagctct tgccattctt tcttgtcagg atcaaagcca tagacaatgt atctttcatc
    23341 atcaaaggca aagaggatcc gctctgccct gccatcacta ttcagatctt gctcgaccag
    23401 tacgcaggcg tctttttcaa tacaggtcat cacgttatag cgatcctgga ttaaggccga
    23461 ccagaatgtc gcatccggtt taccagaacc aggggcaatt aacacatttt cggctaatac
    23521 tttttccgat atctgttgct gaagatgttg ctctccatct aacgccatca gtagatcccg
    23581 cgcgcgtttc gggtctttca tatactcggc atcgcttttc agcgactcaa gcgccgcacg
    23641 tccatagcga ccgctgtgtt cgagcatgta gatagttacc tggtctggcg tgtttttgcc
    23701 gctctgataa cgcgccatat gactgttcac actaatgcgc atactgtcca gcaccggcga
    23761 attaagaagc accagtatta ccaacaccaa taacgaaacc gcaaggttca ctttgccctg
    23821 aagaacaagg ggattttgcc ctgaagaaca aggggatttt gccctttacg ccagacgata
    23881 ctgacaaaat accccagcga ccataccagt aacaccagca ccgccagcgc gccctgcaag
    23941 cggtcgacag tccagccgta ctgagcgacc cgcagccata acgcccaggc ggcgatgaac
    24001 acatacagcg gagcaaccag cagagcggtt ttaatcaggc aacgcaacgg ccccgtccag
    24061 ggaagtgacg ctttttgcgg atcgcggaca atagccatta agatcaattg caaaaaggcc
    24121 agcgttaaca gcaacccggc ggcggagatg tggcgagaaa tcgcactcag gcctgtaaac
    24181 ggcagggtga tgataaacat cagggttagc aatgatacta acggcagcaa ccccgtggcg
    24241 attaacgtga acaacttttg aatagagtcg attaaacgtg actgtgttcg cgcgaggatc
    24301 accgccagcg cggtaaccag acctaacgtg agataaataa accagtcggt tgcaaaaaag
    24361 agcgttttga aaaacgtgat gccaacaagt ttaaacaact cactccacag taaaagcacc
    24421 agccacgtca gaccattggc gagaaaaatc accagtaata taagtacgtt atgccatact
    24481 gactggtaaa aatagcgata gcggctactg tcattgcgga tgcgaaggct ttgttgtatc
    24541 cacggtagca acaacattgc catcagcagc agatagcaac caaaatccca aagcgccttt
    24601 tcagctctcc agggggtcat gccatcagtt tgccacttca gccagccgct catccccagc
    24661 gtggcaataa acaccagcgc cagccatccc caaaggcgct tctgtttaaa agagatcacg
    24721 gagaagagta aaacggatga aaacgcgacg gtcgcaggca cgccataaac aatccaactg
    24781 tgattttttc cggaaagcca ggcgatcagc aggtagcaaa ccagaccctg aagtaatccg
    24841 gtagcaatca tcccccagcg tgttgcgggt gaaagttcaa cgttatccat gttggttatc
    24901 cagaatcaaa aggtgggtta attatcgcat ccgggcagta gtattttgct tttttcagaa
    24961 aataatcaaa aaaagttagc gtggtgaatc gatactttac cggttgaatt tgcatcaatt
    25021 tcattcagga atgcgattcc actcacaata ttcccgccat ataaaccaag atttaacctt
    25081 ttgagaacat tttccacacc taaaatgcta tttctgcgat aatagcaacc gtttcgtgac
    25141 aggaatcacg gagttttttg tcaaatatga atttctccag atacgtaaat ctatgagcct
    25201 tgtcgcggtt aacaccccca aaaagacttt actattcagg caatacatat tggctaagga
    25261 gcagtgaaat gcgcgttaac aatggtttga ccccgcaaga actcgaggct tatggtatca
    25321 gtgacgtaca tgatatcgtt tacaacccaa gctacgacct gctgtatcag gaagagctcg
    25381 atccgagcct gacaggttat gagcgcgggg tgttaactaa tctgggtgcc gttgccgtcg
    25441 ataccgggat cttcaccggt cgttcaccaa aagataagta tatcgtccgt gacgatacca
    25501 ctcgcgatac tttctggtgg gcagacaaag gcaaaggtaa gaacgataac aaacctctct
    25561 ctccggaaac ctggcaacat ctgaaaggcc tggtgaccaa acagctttcc ggcaaacgtc
    25621 tgttcgttgt cgacgccttc tgtggtgcga acccggatac tcgtctttcc gtccgtttca
    25681 tcaccgaagt ggcctggcag gcgcattttg tcaaaaacat gtttattcgc ccgagcgatg
    25741 aagagttggc aggtttcaaa ccagacttta tcgttatgaa cggcgcgaag tgcactaacc
    25801 cgcagtggaa agaacagggt ctgaattccg aaaacttcgt ggcttttaac ctgaccgagc
    25861 gcatgcagct gattggcggc acctggtatg gcggcgaaat gaagaaaggg atgttctcga
    25921 tgatgaacta cctgctgccg ctgaaaggta tcgcttctat gcactgctcc gccaacgtta
    25981 gcgagaaagg cgatgttgcg gtgttcttcg gcctttccgg caccggtaaa accacccttt
    26041 ccaccgaccc gaaacgtcgc ctgattggcg atgacgaaca cggctgggac gatgacggcg
    26101 tgtttaactt cgaaggcggc tgctacgcaa aaactatcaa gctgtcgaaa gaagcggaac
    26161 ctgaaatcta caacgctatc cgtcgcgatg cgctgctgga aaacgtcacc gtgcgtgaag
    26221 atggcactat cgactttgat gatggttcaa aaaccgagaa cacccgcgtt tcttatccga
    26281 tctatcacat cgataacatc gttaagccgg tttcaaaagc gggtcacgca acgaaagtta
    26341 tcttcctgac cgcagacgcc tttggtgtgc tgccaccggt ttctcgtctg actgccgatc
    26401 aaacccagta ccacttcctc tctggcttca ccgccaaact ggccggtact gagcgtggca
    26461 tcaccgaacc gacgccaacc ttctccgctt gcttcggcgc ggcattcctg tcgctgcacc
    26521 cgactcagta cgcagaagtg ctggtgaaac gtatgcaggc ggcgggcgcg caggcttatc
    26581 tggttaacac tggctggaac ggcactggca aacgtatctc gattaaagat acccgcgcca
    26641 ttatcgacgc catcctcaac ggttcgctgg ataatgcaga aaccttcact ctgccgatgt
    26701 ttaacctggc gatcccaacc gaactgccgg gcgtagacac gaagattctc gatccgcgta
    26761 acacctacgc ttccccggaa cagtggcagg aaaaagccga aaccctggcg aaactgttta
    26821 tcgacaactt tgataaatac accgacaccc ctgcgggtgc cgcgctggta gcggctggtc
    26881 cgaaactgta atgatttgaa gctggagaat atctatccag tatcttatag aaagcaaaac
    26941 gggaggcacc ttcgcctccc gtttatttac ccttcttttg tcgtgctctg cgcccgcgtt
    27001 accggcacag gcagccaggc gcgaatggaa agcccgcccc gctcgctggt gccaagctcc
    27061 agcatcccgt tatggttatc cacgatacgc tgcacaatcg ccagccctaa tcccgtgccg
    27121 ctaatggtgc gtgcactgtc gccgcgaaca aacggctgga aaaggtgctt acgttgttcc
    27181 ggcgcaattc ccggaccgtc atcttccacc tggaaccagg cgcgattcgg ctccgtcccg
    27241 ctgctgactt tgatccagcc attgccgtaa cgggcggcgt tgaccaccat attcgccacc
    27301 gcgcgtttga tcgacagcgg gtgcattttc acttcaatgc tgccggggta aagcgcggtt
    27361 tcaatttccc gctcatagcc actttcggca gcaataacct caccgagtac tgcgttgaga
    27421 tccgccattt ccatcggcat ctcctgtccg gtgcgcaggt agtcgataaa ctgctcaatg
    27481 atggcattgc actcctcgat atctttattg atcgattctg ccaggtagcc atcctgctcg
    27541 ctcatcatct cggtcgccag gcgaatacgc gtcagcggtg tacgcaagtc gtgacttacc
    27601 cccgccatca gcagcgtgcg gtcatccgcc agttgcttaa caccagccgc catatggtta
    27661 aaggcgcggg taacggaacg cacctcggac gcgccatact cacgcagcgg cggcggaata
    27721 atccctttac caacctgcaa ggctgcgtgt tcgagatcga ccaacggtcg attctggata
    27781 cgaataaaca gccacgcccc gcctatcgcc aatagcataa tcgccaacgt atagcggaac
    27841 agcggagaga aatcgccctg atgaatttcg gtcagcggca cgcgtaccca gatattgggc
    27901 gacagccagg ttttcagcca gacgacaggc gaacttttgt tgacctcaac gcgcacttcc
    27961 gtcgggccgc ccagttgctg tgccatctga tggcttaaga attcatagtg ttgcgcccaa
    28021 cgcagacctg cctcttcggc agcctcgttg gagtagagag agatccccag ctcacggtag
    28081 atctcccgac ggaaagcggg aggcacaacc aactgcgtgc cgtcctccag ttgcagtttg
    28141 tcggtcatca acatacgcac ttcgtacgcg aggactttat taaactgctg gaggctcggc
    28201 aaaatcgcga agttcagcac caccagataa gtcgtcacca ggctggcgaa cagcaaggtg
    28261 acgatgagca ataacgtacg ggcaaatgaa cttcgtggcg agaagcgcaa tcgcctcatg
    28321 ctttagagcc gtccggtaca aagacgtagc ccaggcccca aacggtctga atgtaacgcg
    28381 gatgcgctgg atcttcttcc accatgcggc gcagacgaga aatctgcacg tcgatggagc
    28441 gttccattgc ggaatattca cgaccacggg caaggttcat cagcttatcg cgggagagcg
    28501 gctcacgcgg atggctgacc agtgccttca gtaccgcaaa ctcaccgctg gtgagcggca
    28561 tcggctcgtc ttcgcggaac atttcgcgcg taccgaggtt aagtttgaac ttaccgaaag
    28621 caattaccgc ctcttcctgt gacggtgcgc ctggtagctc gttcgcctga cgacgcagca
    28681 ccgcacggat acgggccagc agttcacgcg ggttaaacgg ttttggaatg taatcgtcag
    28741 cgccaatctc caggccaacg atacggtcca cttcttcccc tttcgccgtc accataatga
    28801 tcggcatcgg gttgctctga ctacgaagac gtcggcaaat cgacaagcca tcttcaccag
    28861 gtaacattaa atccagtacc ataagatgga aagattcacg agtcagcagg cgatccatct
    28921 gttctgcatt agcgacgctt cgaacctgga agccttgttc ggtgagataa cgttccagca
    28981 gcgcacgcag gcgcatgtcg tcatcgacca ccagaatctt gtagttctct tgcattgttt
    29041 gtactcccaa aggttcgcaa caatttgtaa gcgtgtattc ttaaaaaagc tcacgttcgt
    29101 caccagctaa atctggtatg aatttcagcc taaattgtta caaagcatat taaacagcag
    29161 cttaagtata caatttattc ggcgaaacat tattgattct gttgatatga tcacgttata
    29221 cccaatgtgc gcattatcaa acagacaaag ggaatcaacg agatgaaaac gcccctggtt
    29281 acccgggaag ggtatgaaaa actcaaacaa gagcttaatt atctctggcg tgaagaacgc
    29341 ccggaggtca caaaaaaggt gacctgggcc gcaagtctgg gcgaccgcag cgaaaatgct
    29401 gactatcagt ataataaaaa gcgcctgcgt gaaatcgacc gtcgcgtgcg ctatctcact
    29461 aaatgcatgg aaaatctcaa aatcgtcgat tactccccgc agcaggaagg caaagtcttt
    29521 tttggcgcgt gggtggagat tgaaaacgac gatggcgtga ctcaccgttt ccgtattgtc
    29581 ggctacgatg aaatttttgg ccgtaaagat tacatctcta tcgattcccc gatggcccgc
    29641 gcattgctga aaaaagaagt cggcgatctg gctgtggtga atactcctgc cggggaagcg
    29701 agctggtatg ttaatgctat cgagtacgtg aaaccgtaag gaagagtctt aacctcctgc
    29761 cgatggctgg catttttgcc agccagtccg tataactatc ccctgatttt tgatccgaaa
    29821 agatgaactc aaaccatgat gaatgattcg ttctgccgca ttattgcggg tgaaattcag
    29881 gcgcgcccgg aacaggttga cgctgccgtt cgcctgcttg acgaagggaa taccgtgcct
    29941 tttattgcac gttatcgtaa ggaaatcacc ggcggtctgg atgacacgca gctgcgtaat
    30001 ctggaaacgc gtctgggata cctgcgcgag ctggaagaga gacgtcaggc gatcctcaag
    30061 tccatttccg agcaaggcaa actcaccgat gatctggcga atgccatcaa cgccacccta
    30121 agcaaaaccg aactcgaaga cctctacctg ccctacaaac ctaaacgccg cacccgcggg
    30181 caaatcgcca ttgaagcagg gcttgagccg ttggctgacc tgctgtggag cgatccgtca
    30241 cacacgccag aagtcgccgc tgcgcaatat attgatgccg ataaaggcgt ggcagatacc
    30301 aaagccgcgc tggacggcgc gcgctatatc ctgatggaac ggtttgccga agatgccgcg
    30361 ctgctggcga aagtgcgtga ttatctgtgg aagaacgcgc atttggtttc tacggtggtg
    30421 agcggtaaag aagaggaagg ggcgaaattc cgcgactatt tcgatcatca cgaaccgttg
    30481 tccacggtgc cttctcaccg cgcgctggcg atgttccgtg ggcgtaacga aggcgtactt
    30541 cagctttcgc tgaatgccga tccacagttc gatgagccgc ccaaagagag ctattgcgag
    30601 caaatcatca cggatcacct tggcctgcgc ctgaacaatg ccccggcgga tagctggcgc
    30661 aaaggcgtgg tgagctggac gtggcgcatc aaggtgctga tgcatctgga aaccgaactg
    30721 atgggcaccg tgcgcgaacg tgcggaaaat gaagcaatca acgtctttgc ccgtaacctg
    30781 cacgatctgc tgatggctgc ccctgccgga ctgcgtgcaa cgatgggcct cgatccgggt
    30841 ctgcgtaccg gggtaaaagt ggcggtggtc gatgccactg gcaaactggt agcgaccgat
    30901 accatttacc cgcacaccgg acaagccgca aaagcagcga tgactgttgc tgcgctgtgt
    30961 gaaaagcata acgttgaact ggtggcgatc ggcaacggta cagcttcccg cgaaaccgaa
    31021 cgtttctatc tcgacgtgca gaagcagttc ccgaaagtga ccgcacagaa agtgatcgtc
    31081 agcgaagctg gcgcgtcggt ttactcggct tccgagctgg cagcgcagga gttcccggat
    31141 ctcgacgttt cgctgcgtgg cgcggtttct atcgcccgcc gtttgcagga tccgctggcg
    31201 gagctggtga aaatcgatcc gaaatctatc ggcgtaggtc agtatcagca tgacgtcagc
    31261 cagacgcaac tggcccgcaa actggatgca gtagtagaag actgcgtaaa cgccgttggc
    31321 gtcgatctca acaccgcttc tgttccgcta ttaactcgcg tggcgggcct gacgcgaatg
    31381 atggcgcaaa acatcgtggc ctggcgcgat gagaacggcc agttccagaa ccgtcagcaa
    31441 ctgctgaaag tcagccgtct ggggccgaaa gccttcgagc agtgcgcggg cttcctgcgc
    31501 attaaccacg gtgacaaccc gctggacgcc tctaccgttc acccggaagc ctatccggtg
    31561 gtggaacgca ttctggcagc aacacagcag gcactgaaag atctgatggg taacagcagc
    31621 gaactgcgta acctgaaagc gtctgacttt actgatgaaa aattcggtgt gccgacagta
    31681 actgacatca tcaaagagct ggaaaaaccg ggtcgcgatc cgcgtccgga atttaaaacc
    31741 gctcagtttg ccgatggcgt cgagacaatg aacgacctgc aaccgggtat gatcctcgaa
    31801 ggcgctgtca ccaacgtcac caactttggc gcgtttgtcg atatcggcgt gcatcaggac
    31861 ggcctggttc acatctcttc attgtcgaac aagtttgtgg aagatccgca taccgtggtg
    31921 aaagcgggcg acattgtgaa ggtgaaagtg ctggaagtgg atcttcagcg taaacgtatc
    31981 gccctgacta tgcgtctgga cgagcagcct ggcgaaacca actctcgtcg cggcggcggt
    32041 aacgatcgcc cgcaaaacaa ccgcccggca gccaaaccac gcggtcgtga agcgcagcct
    32101 gccggtaaca gcgcgatgat ggacgcgctg gcggcagcaa tgggcaaaaa acgttaaacg
    32161 cccggactgg cccctaaacc tgcgcgataa ggcgttcacg ccgcatctgg caacgaacgc
    32221 cttgcctgac ataaaagtgc cggagaatat ctccggcata tttattccac agccaaactc
    32281 acaatatatt tcgttaatat ttatcacttc attaacaact gaaaccttaa ttaaacatta
    32341 gccagtcctg gtaattcgct attcgaatta tattttcgct gcgatataac cttgagccac
    32401 atcaacattg agtcagatta ttattcaaac caacattcgc acacatttta agtattgctg
    32461 atagaaacca ttctcattat cattgtacta ttgattattt aatctctcct tcgttggcaa
    32521 atcatctggt ctcatgtcgc tgtcaaacgc cccatgaggt agttatccag ttaatgagaa
    32581 acaagtaggc acctatgcaa tacactccag atactgcgtg gaaaatcact ggcttttccc
    32641 gtgaaatcag cccggcatat cgccaaaaac tgctttctct tggcatgtta cctggctcct
    32701 cttttaatgt ggtgcgcgtc gctccactcg gcgaccccat tcatatcgaa acccgtcgtg
    32761 tgagcctggt attacgcaaa aaagatctgg ccttattaga agtggaagcg gtttcctgtt
    32821 aatacggtga taacaacaat gaaaaaatta accataggct taattggtaa tccaaattct
    32881 ggcaagacaa ccttatttaa ccagctcact ggcgcacgtc agcgtgtagg taactgggct
    32941 ggcgttaccg tcgaacgtaa agaagggcaa ttctccacca ccgatcatca ggtcacgctg
    33001 gtggacctgc ccggcaccta ttctctgacc accatctcat cgcagacctc gctcgatgag
    33061 caaatcgcct gtcactacat tttgagtggc gacgccgacc tgctgattaa cgtggtggat
    33121 gcgtctaacc ttgagcgtaa cctgtacctg acgctacaac tgctggaact cggcattccc
    33181 tgcatcgtgg cactgaacat gctcgacatt gccgagaagc aaaatattcg tattgaaatt
    33241 gatgctctgt cggcgcgtct tggctgccca gtgatcccgc tggtttcaac ccgtggtcgc
    33301 ggtattgaag cgctcaagct ggcgattgat cgctataaag ctaacgagaa tgtggaactg
    33361 gtgcattacg cacagccgct actcaacgaa gcagattcac tggcaaaagt gatgccttcc
    33421 gacatcccgc tgaaacaacg tcgctggctg ggcctgcaaa tgctggaagg cgatatctac
    33481 agccgcgcct acgccggtga agcgtcgcag catcttgatg ccgccctcgc ccgtctgcgt
    33541 aatgagatgg acgatccggc gctgcacatt gccgatgcgc gttaccagtg cattgctgcc
    33601 atctgtgatg tggtaagcaa caccctgacg gcagaaccca gccgtttcac cactgcggta
    33661 gataaaatcg tgctcaaccg tttcctcggt ctgccgattt tcctctttgt gatgtacctg
    33721 atgttcctgc tggctatcaa catcggcggg gcgttacagc cgctgtttga cgtcggctcc
    33781 gtggctctat ttgtgcatgg tattcaatgg attggctaca cactccactt cccggactgg
    33841 ctgactatct tcctcgccca gggcctgggt ggcggtatta acaccgtgct gccactggtg
    33901 ccgcagatcg gcatgatgta cctgttcctc tccttccttg aggactccgg ctatatggcg
    33961 cgtgcggcgt ttgtgatgga ccgcctgatg caggcgctgg gcttgccggg gaaatccttt
    34021 gtgccgctga tcgtcggttt cggttgtaac gtaccgtcgg taatgggtgc acgtacgctt
    34081 gatgcaccgc gtgaacgtct gatgaccatc atgatggcac cgtttatgtc ctgcggcgcg
    34141 cgtctggcta tcttcgcagt attcgcggct gccttcttcg ggcagaacgg tgcgctggcg
    34201 gtcttctcgc tgtatatgct cggtatcgtg atggcggtgc tgactggcct gatgctcaag
    34261 tacaccatca tgcgcggtga agcgacgccg tttgtcatgg agctgccggt ctaccatgta
    34321 ccacacgtta aaagcctgat tatccagacc tggcagcgtc tgaaaggctt cgttctgcgt
    34381 gctggtaaag tgattatcat cgtcagcatt ttcctgagcg ctttcaacag cttctcgttg
    34441 agcgggaaaa tcgtcgataa catcaacgac tcggcgctgg cttccgtcag ccgggtgatc
    34501 accccggtct tcaagccgat tggcgtgcat gaagataact ggcaggcaac agttggcctg
    34561 tttacgggtg caatggcgaa agaagtggtg gtgggtacgc tcaacaccct ctacaccgca
    34621 gaaaatattc aggacgaaga gttcaatccg gcggaattta acctcggtga agagttgttc
    34681 agcgcggtag atgaaacctg gcagagcctg aaagacacct tcagccttag cgtactgatg
    34741 aaccccattg aagctagcaa aggcgacggc gaaatgggta ccggggcgat gggcgtgatg
    34801 gatcagaaat tcggtagcgc agctgccgca tacagctacc tgattttcgt cctgctgtat
    34861 gtaccatgta tctcggtgat gggagccatc gcccgtgaat caagccgtgg ctggatgggc
    34921 ttctccatcc tgtgggggct gaatatcgct tactcactgg caacattgtt ctatcaagtc
    34981 gccagctaca gtcagcaccc aacttacagc ctggtgtgca ttctggcggt tatcctgttt
    35041 aacatcgtgg ttatcggtct gctgcgccgc gcgcgtagcc gggtggatat cgaactgctg
    35101 gcaacccgca agtcggtaag tagttgctgc gcagccagca ccaccggtga ttgccattaa
    35161 tggcttcact tattcaggtg cgtgatttgc tggcgttacg gggccgtatg gaagcggccc
    35221 agataagcca gacattgaac actccacagc caatgattaa cgccatgctg caacaactgg
    35281 aaagtatggg caaagccgtg cggattcagg aagaacctga cggctgcctc tccggcagtt
    35341 gtaaaagctg cccggaagga aaagcctgtc tgcgcgagtg gtgggcgctg cgttaaccat
    35401 actccatcgc ctgttttgaa aagcccggta tgcgtttgca tccgggcttt tttgcgtgcg
    35461 gctttccata aaaatgcaat tcttgcagcg cgacgtaagt tcctctgaaa gcgtctcgca
    35521 gggatgaaaa ctggctaatg cacaggtgtg gagtggcgcg tagagtcgcg gcattcaaac
    35581 aacaggtgaa ggaacgccat gagcaaaaag cagagttcca ccccacacga tgcgctgttc
    35641 aaactctttt tacgccaacc ggagacggca cgcgattttc ttgcgtttca tttaccagca
    35701 cccattcacg cgctctgtga tatgaaaacc ctcaagctgg agtcgagcag ctttattgat
    35761 gacgatctgc gtgaaagcta tcccgatgtg ctgtggtcgg tgaaaacaga acaaggacca
    35821 ggatacatct attgtctgat tgaacatcaa agcacctcaa acaaactgat cgcatttcgc
    35881 atgatgcgtt acgctattgc cgcaatgcaa aatcaccttg atgccggata caaaacgttg
    35941 ccgatggtgg tgccattgtt gttttaccac ggtattgaaa gcccctatcc ctattcgctg
    36001 tgttggctgg attgtttcgc cgatcccaaa ctggcaaggc agctttatgc ctccgcattt
    36061 ccgctgattg atatcaccgt catgcctgat gatgaaatca tgcagcaccg acgcatgacg
    36121 ctgctggagt taattcaaaa acatattcgt caacgcgacc tgatggggct ggtagagcaa
    36181 atggcctgct tattaagtag tggatacgct aatgacagac aaatcaaagg gctgtttaat
    36241 tacatactgc aaaccggcga tgcggtacgt tttaacgatt ttatcgacgg cgttgccgaa
    36301 cgatcaccga aacacaagga gagtttaatg actattgcgg aaagattgcg gcaggagggg
    36361 gaacaatcca aagccctgca tatagccaaa ataatgcttg aatccggagt ccctcttgca
    36421 gacatcatgc gctttaccgg gctgtcagaa gaagagttgg ctgcggcgag tcagtaaagt
    36481 tctgtctcgc cattcaaaag tgcctacacc ctctgcttca acgccaccag cacgtgacga
    36541 aactcgaccg gatgcgaaat aaacggcgca tgagccgctt tggcgaagat gtatgactcg
    36601 ctgtgtggcc agagtttatc cagcatcggc accactttgc gcggcaccag accatcgaga
    36661 tagccataca gtcgcaaaaa cggcatgggt acgttttgca gcggcaagcg gagatcaacc
    36721 gttttgagga tttccagccc accattgagc acgtcaacct ccggcatcgg taacgccaga
    36781 acggttttct tcaacgcccg cgcatcctga cgcgccgttt cagtacccat ggtttgtaac
    36841 gcaaggaacc gttccaccgt acgctgaaaa tcatcactga gttgctgctg aaatcccgcc
    36901 agcacgtccg gttttatccc cggccactcg tcacgagcac taaaacaagg tgacgacgcc
    36961 acggtgacca gcgcctgaac acgctcggga tgggttaacg caatctggct tgccaccagc
    37021 ccgcccagac tccagcctaa ccaaatggct ttatcaggtg cctgtcgcag cacggcttcg
    37081 gccatctccg caagtgacag cgcaccaaat ccccggctac gcccgaagcc gggcaggtca
    37141 acaagatgca gcgtaaaatg cgagctaagt tcctcgtcaa tgcaacgcca cacttcagca
    37201 ttcagtcccc atccgtgcag cagcacaaga tgaacattcc cctgaccttt ggtctgccac
    37261 cagatgttat tcatccgcta ttgttctctt ttgacttaca aggatgaaca tatgctaaca
    37321 gtaccgggat tatgctggct atgccgaatg ccactggcat taggtcattg ggggatttgt
    37381 tcggtctgct cacgcgccgc ccgtacagat aaaacgttat gcccacaatg tggattaccc
    37441 gccacacact cccatcttcc ctgcggtcgc tgcctgcaaa aaccgccgcc ctggcaaaga
    37501 ctggtcacgg ttgccgacta tgcgccgccg ttaagtctgc tcatccacca gcttaagttt
    37561 tcccggcgca gcgaaatcgc cagcgccctg tcacgtctgt tactattgga agtcttacac
    37621 gctcgtcgca ccaccgggtt gcaattgccg gatcgtatca tcagcgttcc gttatggcag
    37681 cggcgtcact ggcgtcgggg atttaatcag agcgatttgc tgtgtcagcc gttatcacgc
    37741 tggttgcact gccaatggga tagcgaagcc atcacacgta cacgggccac tgcgacccag
    37801 cattttctta gcgcccggct acgcaggcgc aacctgaaaa atgcctttcg ccttgaattg
    37861 cctgtgcaag gtcgccatat ggtgattgtg gatgatgtcg ttaccaccgg aagtaccgtc
    37921 gcagagattg cgcagttgct tttacgcaat ggtgcggcga ctgtccaggt ctggtgcctt
    37981 tgtcgaacct tgtagagcct cgatgatggg cgtattataa ccaactaaaa tagtcaacta
    38041 ttaggccatt actatgatcc gtatttccga tgctgcacaa gcgcactttg ccaaactgct
    38101 ggcaaatcag gaagaaggga cacaaatccg cgtatttgtg attaaccctg gcacgcctaa
    38161 cgctgaatgt ggtgtttctt attgtccgcc ggatgccgtg gaagccaccg acaccgccct
    38221 gaaatttgac ctgctgaccg cgtatgttga tgagttaagc gcaccatacc tggaagatgc
    38281 agagatcgat tttgttaccg accagttggg ttctcagcta acactgaaag cgcctaacgc
    38341 caaaatgcgt aaagtggcag acgatgcacc actgatggag cgcgtggagt atatgctgca
    38401 gtcgcagatc aacccacagc ttgctggtca cggtggccgc gtttcactga tggaaatcac
    38461 cgaagacggt tacgccattc tgcaatttgg cggcggctgt aacggttgtt ccatggtcga
    38521 tgtgacgctg aaagaaggga tcgagaagca gctactaaac gaattcccgg agctgaaagg
    38581 tgtgcgcgat ctcaccgaac accagcgcgg cgaacactcc tactactaag ttatcccctc
    38641 ttttgtagat tgcccgatgc gacgctaaag cgtcatttcg ggcctacgtt gaacatgtgc
    38701 cagacgttgg cgcagcattg ctttggcaaa tccctccctt cgtttccccg cgataatatg
    38761 accaacctct cataatttaa atttaccccg ctctggtgat tctcaaacgc cagatgttac
    38821 ccgtatcatt cacgtgggta ccaaacatac tcctgacatc tgactacaat aattagtttt
    38881 agtgggtatc agtcgtggtg ccgcaatatc tctgttcccg attgggataa ttagagcttg
    38941 tcgtcagaaa attgacgtta cccataacaa atgaaaggcc aggtaaatca tgccattagt
    39001 cattgttgct atcggtgtaa tcttgttatt actcctgatg atccgcttca aaatgaacgg
    39061 cttcatcgct ctcgtcctcg tggcgcttgc ggttggatta atgcaaggga tgccgctgga
    39121 taaagttatt ggctccatca aagccggtgt cggcgggacg ctcggtagcc ttgccctgat
    39181 catgggtttt ggcgcaatgc tgggcaaaat gctggcagac tgcggtggcg cacaacgtat
    39241 cgccaccacg ctgattgcca aatttggtaa aaaacacatc cagtgggcgg tggtactgac
    39301 cggttttacc gttggttttg ccctgttcta tgaagtgggc tttgtgctga tgctgccgct
    39361 ggtgtttacc atcgcggctt ctgcgaatat tccactgctg tatgttggtg taccaatggc
    39421 agctgcactg tctgtgaccc acggcttcct gccaccgcat ccgggtccga ctgcgattgc
    39481 caccattttc aatgccgata tgggtaaaac cctgctgtac ggtactattc tggcaatccc
    39541 gaccgtgatt ctcgccggtc cggtttacgc tcgcgtgttg aaaggtatcg ataagccaat
    39601 tccggaaggt ctctacagcg cgaaaacctt cagcgaagaa gagatgccga gctttggcgt
    39661 cagcgtctgg acctctctgg tgccggtagt actgatggcg atgcgtgcga ttgccgaaat
    39721 gatcctgccg aaaggtcacg ctttcctgcc ggtagcggag ttcctcggtg acccggtaat
    39781 ggctacgctg attgccgtgc tgattgcgat gttcaccttt ggtctgaacc gtggtcgttc
    39841 aatggatcag attaacgaca cgctggtttc ttccatcaaa atcattgcga tgatgctgtt
    39901 gatcatcggt ggtggcggtg cgttcaagca ggtgctggta gacagcggcg tggacaaata
    39961 cattgcttcc atgatgcacg aaaccaacat ttctccgctg ctgatggcct ggtcgattgc
    40021 tgccgtactg cgtatcgcgc tgggttccgc aaccgttgcg gcaatcactg cgggtggtat
    40081 cgcggcaccg ctgattgcaa cgacgggtgt tagcccggag ctgatggtta ttgcggtggg
    40141 ttccggtagt gtgattttct ctcatgtgaa cgatccgggc ttctggctgt tcaaagagta
    40201 ctttaacctg actatcggcg agaccatcaa atcctggtcg atgctggaaa ccattatctc
    40261 ggtgtgcggt ctggtaggct gtctgctgct gaatatggtg atttgataac actgactgcc
    40321 ggatgcatcg catccggcat caaccgcact ctacttcttc ttcgctgcag ctctgcgccg
    40381 tctgtccaaa tccttcagca acttgttcac gccatcatcg gcaaacatcg tctcaagcgt
    40441 tgcggaaagc ttgcgtcgcc agtttttata ctggtaactg gtgccaggaa tattcaccgg
    40501 ttcggccata tccagccagt cttccggctg tagtcctaac agagcactgt tactgtcggc
    40561 aatgtagcgc tgcaaaccac gattaagcgt cggcgtcatc gacatcaacg atgccttatg
    40621 cccggcacgt tttggcagac aaccatattt atgcagtgca tccagcagcc cttgcttcgc
    40681 cagttcgcga tcctgataca gaccgcgcag taccacttca tccggataca gccccagggt
    40741 tttgcccagc gttagatccc cgctctccca gtagccgcgt agcgtaggca ggtcatgtgt
    40801 cgccgcaacc gccatcgact gctccggata cgctttcggt gcacggaacg tcttctcgtg
    40861 gtcgttttcg aaatagagca ctttgtagga gtacacaccg ctgctgcgca gcttaccgac
    40921 aatctctacc ggtacggtac cgagatcttc accaatcacc atacagcgat gacgtttact
    40981 ttcgagtgcc agaatcgaga gcagatcatc caccggatag tgaacatacg cgccctgatc
    41041 tgccgtctcg ccatacggta tccaccacaa acgcagcatc gacatcacat ggtcaattcg
    41101 taatgcgccg cagttttgca tattggcacg caacagctcg ataaacggtt cataagcacg
    41161 cgcggtgatg atatgcgggt ccattggcgg caatccccag ttctgcccca acgggccgag
    41221 gatatccggc ggcgcgccaa ccgacgcttt caggcaatac agttcacggt cgcaccaggt
    41281 ttccgcccca ccttccgcta cgccaaccgc cagatcacga tacaagccaa tcggcatttc
    41341 atagccctgg cttatctccc agcaggcggc aaactggctg taagcctgcc actgcaacca
    41401 gagataaaaa tcaacgtcat cgcgatgttc ttcgcagaac tgacgcactt ctggtgaatc
    41461 cacgttctga tacatctctg gccatgcagg ccagccccag cgcatttcgt cctctttcac
    41521 ttgctgggca tgtagcgcat caaaggctgc ctgccagaac aggctgtcgc cttgatctgc
    41581 aacaaactgg cgaaacgcgg ccatctgctc atcatcacgt tgcgcgaaac ctttccacgc
    41641 cattcgtaat gctgtcattt ttagggcggt aaccgtggag taatcgaccc agtcggcatc
    41701 ccgcgcctgt tgcagcgtct gttgcgtggt cggcaactgc caccaggcct gagcctcttc
    41761 gctaagatgg aaatcttcga cggcgttaac gtcgatataa atcacattca gccagcggcg
    41821 agaagacggg ctatacgggc tggcgctctc cggatttgcc ggatagagcg catgaatcgg
    41881 gttcagacca atgaacgatc cgccacgttt tgccacatcc actagcatcg ctttgagatc
    41941 gccaaaatcc ccaatacccc agtttttttc cgatcgcagc gtataaagct ggacgcaggc
    42001 accccacagc ttttgtttat tcagcaacgc ctgcggttcg taacagcgtt tcggggcgac
    42061 aatcaccctg caatgcccgc gctggtcttc ctgggtaagc gtcagcgtgt gataaccttc
    42121 cggcagcttc gtcggtagat tgaacgcttt gccccccgtt atatggcctt tgtactgcgt
    42181 tccttcttcg gtggtcagca gccagctata ttcgccgctg ccctccacca ccatcggcat
    42241 ttttttgccg ctggtataaa ccatgacatt cgggactggc gttaccgcca ctttcgtggc
    42301 ggtacgttga tgcatcgcgt caagcaaacg ccgtttggtt tcggcgctaa tcgactgcgg
    42361 tttaccgtgg gcattgatgt aatgagggct aatccccgcc gccagcgcgg cattatccag
    42421 acgtttgctt tccatcgagc ttccttagcg ttttgcctgc cagatacgag cctgataatc
    42481 gcgaatagag cgatccgagc taaacatacc gcagcgggcg gtattgagga tcgccgcgcg
    42541 agtccaggcc tcctggtcgc ggtacagcac atccacctgc ttttgtgcct ctacataggc
    42601 tgcgaaatcc gccatcacca gatacggatc gccgccctgt ttgccgatac tgtgcagcat
    42661 ctggtcgaag gcatgcttat cgccgtcgct gtatttaccg ctttccagct ctttcaatac
    42721 tgcgtccagc accttatctt tcttccgcca tttcaccggg tcgtagcctt tggccagaat
    42781 tgccttcact tgttccacgg tatgaccaaa aataaagata ttttcttcac cgactttctc
    42841 ggcgatttca acgttcgccc catccagcgt accgacagta agcgcaccat tgagcgccag
    42901 tttcatattg ccggtaccgg aagcttcttt acctgcagtc gaaatttgtt cggagatatc
    42961 cgccgccggg atcagttttt ccgccgccga aacgcaataa tccggcagga acaccacctt
    43021 caacttatcg ccaaccagcg gatcgttgtt gatcacgtca gccactttgt tgatcgcaaa
    43081 gataatattt ttcgccaggt agtagcccgg tgccgctttc gcgccgaaga ggaagacgcg
    43141 cggtacgcga tcagcctgtg ggttttcacg aatttctttg tacaacgcca gaatatgcag
    43201 cagattcagg tgctggcgtt tgtactcatg cagacgtttg atctgaatat cgaaaatcgc
    43261 ctgtggattg atctcaatac cggtacgaac tttcacaaac tccgccagac ggactttatt
    43321 cgcctgcttg atctcgcgat attgctgacg gaatttcgca tcatcagcga atttttccag
    43381 attgatcagc tgatcgagat cgttagccca ctcttttttc agtgatttat ccaacagagc
    43441 cgccagtgcc gggttgcact gtttgatcca gcgacgtggg gtaataccgt tggtgacgtt
    43501 atggaatttg ttcggccata gctggtgata ttccgggaac agatctttca ccaccagatc
    43561 cgagtgcagc gccgcaacac cgttcaccgc gaaaccgcca accacacaca ggttcgccat
    43621 atgcacttgt ttgtcgtgca ccaccgccag tttagcccac actttttcat cgcccggcca
    43681 ggttttctcc accagcgttt taaagcgagt attaatttcg ttaataatct gcatgtggcg
    43741 cggcagtaag cctttcacca gtttcacatc ccagcgttcc agcgcttctg gcatcagggt
    43801 atggttggtg taagcgaaag ttttgctggt aatggcccag gcgtcatccc agctcatctg
    43861 gtgctcatcg atcagcacgc gcaacagttc tggaatcgcg atagttgggt gggtatcgtt
    43921 cagctgaata acttcgtaat ccgccagttc gtgcagttta cgccccgcca gatgatggcg
    43981 acgcaaaata tccgctaccg aacaggcaca ctggaagtac tgctgcatca agcgcagctt
    44041 tttaccggca gtatggttgt cgtttggata gagaactttg gtcagttttt ccgcattgat
    44101 gccctgctgt tcggcacgca ggaaatcacc gtcgttaaat ttagtcagat caaacggatg
    44161 cgcgtgcgtc gcctgccaca gacgcagcgg ctgcgccacg ccattacgat agccgacaac
    44221 ggggagatcc cacgcttgac cggtaatggt aaactccggc tcccagcgtc cgtctttcgt
    44281 cactttaccg ccaatcccta cctgcacatc cagtgcttcg ttgtggcgga accacgggta
    44341 gttactgcga tgccagtcat ccggcgcttc aacctgtttg ccatccacaa aagactggcg
    44401 gaacaaacca tattgatagt tcagaccgta acccgtcgca gactgaccga cagttgccat
    44461 tgaatcgagg aagcacgccg ccagacgtcc cagaccaccg ttacccagcg cagggtcgat
    44521 ctcttcttcc agcaggtcag tcagattgat gtcataagcc ttcaacgaat cctgtacatc
    44581 ctgataccag ccgagattca acaggttgtt gcccgtcaga cgaccaatca aaaactccat
    44641 tgagatgtag ttaacatgtc gctgattcgc caccggcttg gcgaatggct gagcacgcag
    44701 catttcggcc agtgcttcac tcactgccag ccaccactgg cgaggagtca tttcagccgc
    44761 agaatttaag ccataacgct gccactgacg tgaaagcgct tcctgaaatt gcttatcgtt
    44821 aaaaataggt tgtgacatag gagttccact tttcttagat tttcaacaca acgttatcgc
    44881 tagtttgcca ggctcgatgt tgaccttcct catcctgcgg gggattaggc agggaggagt
    44941 tgcggggatg agcaaggaaa tgtgatctcg accacttaaa gctagtgcaa accacaggat
    45001 tagcagcaaa tcaatgccat acagcgcaga aaatctgtat ctaagtgcaa aaaatggccg
    45061 ttgcgtattt tcaaaaagcg aaaggtaact ctataaatta agtaaaggag tgaaacagtt
    45121 tcacaagtaa aatatccagt gtgctccatc tcattcttaa tagatttatt aagatcatct
    45181 ttttagatag cactttcatc aggaatgaag aagaaaccct tgcttaaatg aatctgatga
    45241 acataaggga aaccagtatt cacgctggat cagcgtcgtt ttaggtgagt tgttaataaa
    45301 gatttggaat tgtgacacag tgcaaattca gacacataaa aaaacgtcat cgcttgcatt
    45361 agaaaggttt ctggccgacc ttataaccat taattacgaa gcgcaaaaaa aataatattt
    45421 cctcattttc cacagtgaag tgattaacta tgctgattcc gtcaaaatta agtcgtccgg
    45481 ttcgactcga ccataccgtg gttcgtgagc gcctgctggc taaactttcc ggcgcgaaca
    45541 acttccggct ggcgctgatc acaagtcctg cgggctacgg taagaccacg ctcatttccc
    45601 agtgggcggc aggcaaaaac gatatcggct ggtactcgct ggacgaaggt gataaccagc
    45661 aagagcgttt cgccagctat ctcattgccg ccgtgcaaca ggcaaccaac ggtcactgtg
    45721 cgatatgtga gacgatggcg caaaaacggc aatatgccag cctgacgtca ctcttcgccc
    45781 agcttttcat tgagctggcg gaatggcata gcccacttta tctggtcatc gatgactatc
    45841 atctgatcac taatccagtg atccacgagt caatgcgctt ctttattcgc catcaaccag
    45901 aaaatctcac cctggtggtg ttgtcacgta accttccgca actgggcatt gccaatctgc
    45961 gtgttcgtga tcaactgctg gaaattggca gtcagcaact ggcatttacc catcaggaag
    46021 cgaagcagtt ttttgattgc cgtctgtcat cgccgattga agccgcagaa agcagtcgga
    46081 tttgcgatga cgtttccggt tgggcgacgg cactacagct aatcgccctc tccgcccggc
    46141 agaataccca ctcagcccat aagtcggcac gccgcctggc gggaatcaat gccagccatc
    46201 tttcggatta tctggtcgat gaggttttgg ataacgtcga tctcgcaacg cgccattttc
    46261 tgttgaaaag cgccattttg cgctcaatga acgatgcact catcacccgt gtgaccggcg
    46321 aagaaaacgg gcaaatgcgc ctcgaagaga ttgagcgtca ggggctgttt ttacagcgga
    46381 tggatgatac cggcgagtgg ttctgctatc acccgctgtt tggtaacttc ctgcgccagc
    46441 gctgccagtg ggaactggcg gcggagctgc cggaaatcca ccgtgccgcc gcagaaagct
    46501 ggatggccca gggattcccc agcgaagcga ttcatcatgc gctggcggca ggcgatgcgc
    46561 tgatgctgcg cgatattctg cttaatcacg cctggagttt gttcaaccat agcgaactgt
    46621 cgctgctgga agagtcgctt aaggccctgc cgtgggacag tttgctggaa aatccgcagt
    46681 tggtgttatt gcaggcgtgg ctgatgcaaa gccaacatcg ctacggcgaa gttaacaccc
    46741 tgctggcccg tgctgaacat gaaatcaagg acatcagaga aggcaccatg cacgcagaat
    46801 ttaacgctct gcgcgcccag gtggcgatta acgatggtaa tccggatgaa gcggaacggc
    46861 tggcaaaact ggcactggaa gagctaccgc cgggctggtt ctatagccgc attgtggcaa
    46921 cctcggtgct gggtgaagtg ctgcactgca aaggcgaatt gacccgctca ctggcgctaa
    46981 tgcagcaaac cgaacagatg gcacgccagc acgacgtctg gcactacgcc ttatggagtt
    47041 taattcagca aagtgaaatt ctgtttgccc aagggttcct gcaaaccgcg tgggaaacgc
    47101 aggaaaaagc gttccagctg atcaacgagc agcatctgga acagttgccg atgcatgagt
    47161 ttctggtgcg catccgagca caactgttat gggcctgggc acggctggat gaagccgaag
    47221 cgtcggcgcg tagcgggatt gaagtcttat cgtcttatca gccacagcaa cagcttcagt
    47281 gcctggcaat gttgattcaa tgctcgctgg cccgtggtga tttagataac gcccgtagcc
    47341 agctgaaccg tctggaaaac ctgctgggga atggcaaata tcacagcgac tggatctcta
    47401 acgccaacaa agtccgggtg atttactggc aaatgaccgg cgataaagcc gccgctgcca
    47461 actggttgcg tcatacggct aaaccggagt ttgcgaacaa ccacttcctg caaggtcaat
    47521 ggcgcaacat tgcccgcgca caaatcttgc tgggcgagtt tgaacctgcg gaaattgtcc
    47581 tcgaagaact caatgaaaat gcccgcagtc tgcggttgat gagcgatctc aaccgtaacc
    47641 tgttgctgct aaatcaactg tactggcagg ccggacgtaa aagtgacgcc cagcgcgtgt
    47701 tgctggacgc attaaaactg gcgaatcgca ccggatttat cagtcatttt gtcatcgaag
    47761 gcgaagcgat ggcgcaacaa ctgcgccagc tgattcaact caatacgctg ccggaactgg
    47821 aacagcatcg cgcacagcgt attctacgag aaatcaatca acatcatcgg cataaattcg
    47881 cccatttcga tgagaatttc gttgaacgtc tgctaaatca tccggaagtg ccagaactga
    47941 tccgcaccag cccgttgacc caacgtgaat ggcaggtcct ggggctgatt tactccggtt
    48001 acagcaacga acaaatagcg ggtgaactgg aagtggcggc aaccaccatc aagacgcata
    48061 tccgcaatct gtaccaaaaa ctcggcgtcg cccaccgtca ggctgccgtg cagcacgcgc
    48121 agaaactgct gaagatgatg ggttatgggg tgtgagtttg tatagcgttt ttatgtacac
    48181 tctttatact gtatataaaa tcgcactaca ggctcatgaa tatgaaaccg gaaagcaaag
    48241 aagcgccaat caatatcaga gcgaaagcct cacagcgtga cctgattgat atggcagcga
    48301 atctggtcgc aaaatcgcgc actgatttta tgctggatgc cgcatgccgt gaagcgcaag
    48361 acatcttatt agatcaacga ctattcatcc ttgatgacga gcaatacgat gcgtttctcg
    48421 ctgccctgga cgcccccatc actgctgagc gtcaggccaa aattaacgcg ttgatgaatc
    48481 gtaaatcgcc atgggaataa cggctcctac tcccctaacg tctgaacata atctggctga
    48541 cttttgctgt tccgatcacg ggatgaatga atggctaaaa aagaaagcgt taaaaaatca
    48601 cagctccggt ctgtctcgtg tttacgttat ctgtattgcc aatacccgcc aggtaatcgg
    48661 ctattactgc ctttctacag gcagtattca acgtaatttg gcccctggag ccatgcgacg
    48721 taatgctccc gaatcgttgc ctgttgtcgt attaggcaga ctggcaattg atcaagcctg
    48781 ggcaggtaaa gggttgggcg tcgcattgtt gaaagatgca gtctatcgca caatgtccat
    48841 agctcaacag gtaggggttc gtgcgctcat tgttcatgcc ttagacgatt cagtacgcaa
    48901 tttctatctg aagtatgcct ttgtaccttc accattccag tcacttacct tactgtatcc
    48961 cattactctg gagtaattta cctctaccgg atagtcacac tgatgagtct gatccgggag
    49021 ggatttcaat cagtgagttt ggttatcatc acccgcgtta cgccatctgt ttcagccaga
    49081 gtaaaccgca ccggcaagaa acgctccacc acggcgatat tggtcagcag atggcatgag
    49141 ggatgggcga ccgtaaattg tcccgcgccc gccagcgcca ttggcaacac tagctggtca
    49201 gcaagatatt cccccactgc cgccgggctt gccaggtagc gtttcacctc tttcaccaac
    49261 tgtgccgcaa ccacctcggc actgacgcgc ttttcaccga cgacaaaaaa gcgttcggtg
    49321 atattttcac tttcgacttc aagcgagacg gtattacccg gcccctggtc gcgcggcagg
    49381 ttatgaatat tctgctcatg cagggaaaaa cttgccgcca gcgtagcgat ttcacgctca
    49441 gcgacatgcc gcggcacgcc agctaataac acctctccac gcatctgcac aatgttcccg
    49501 cgctcgccaa gttgcaaggt gttaaacgat gccaccggcg agacttccgt tgccaccacg
    49561 ccgcctccgg caggataaaa accgtgacgt aacagcgtgg tttgctgatg aattcctatt
    49621 ttcgccagca gcggctccaa cacccggcgg ataaaatccg caggcggggc cgacgggtta
    49681 tcggtaccgc cgctcacttc aacacgcgaa ggtccatcgg caaaccacag cgcgggcagc
    49741 accgtttgca gcaccagcgt acaacttcct gcgctaccga tagcaaagcg ataatcgccg
    49801 ccacgcacgg tgccgggccg gaagaccaga cgctgcgacc ccagctccgc gccttccacc
    49861 gtcgccccgc aaatttccgt cgccgccttt accgccgtca gatgctggcg caacagtccc
    49921 ggttttgccc gcccggcacg aatgccggtg atggtaaatg gctggccggt tatcatcgac
    49981 agactcagcg ccgagcgcat gatctgcccg ccgccttcgc cctgtgcgcc atccagcgca
    50041 atcatccttt tcatcattta tccttttacg cacaccacct gacgtagggt gtagataact
    50101 tccaccagat cgctttgtgc cgccatcacc gcatcaatat ctttatacgc catcgggatt
    50161 tcgtcgatca cttcggcatc tttacggcat tccacatgca cggtggcacg aatttgatct
    50221 tccacgctga acattttttt cgctttagta cggctcataa cccgcccggc accgtggctg
    50281 cacgaacaga acgactcttc atttcccagc ccacggacga taaagctttt cgctcccatc
    50341 gaccctggaa taattccata ttgaccagca cgcgcagaca ccgcgccttt acgcgtcacg
    50401 tagatctctt caccaaagtg ctgttctttt tgcacatagt tgtggtgaca gttgatctct
    50461 tccatcgcca gcgtttgtgg ctgtctgacc gttttctgcg taatgctctg caacgccgtt
    50521 actacgtttt ccatcatcac atcgcggtta aggctggcaa aaagctgcgc ccaggccacg
    50581 gctttcaggt aatcatcaaa gtattccgta ccttccataa agtacgccag atcacgcgac
    50641 ggcaatgttt caagcgtttc ctgcatctct ttttgcgcca gatcgataaa gtaagtcccg
    50701 atggcattac caattccgcg tgaaccggag tgcagcataa tccacacctg gtccgactca
    50761 tcaaggcaga tttcaataaa gtggttaccc gttcccagcg ttcccaggtg tttatagttg
    50821 ttagtgttaa ggaaacgcgg atatttttgc gttaaccact gataaccggc ctccagctca
    50881 gcccatttcg catcgacgtt aacaggcggg ttttcccatg cgcctttatc acgtttacaa
    50941 cggccagtgg tacgcccgtg cggcaccgca gtttcaatcg cctgacgcag ctccgccagg
    51001 ttttccggca ggtcagccgc cgttaaagcg gtacgtagtg cgttcattcc acagccaata
    51061 tccacgccca ccgccgccgg aataatcgcc cctttggtcg ggatcacgct accaatggtg
    51121 gaacctttac ccaggtgtac atcaggcatt accgcaatat gtttgaaaat aaacggcatc
    51181 ttcgccgtat taataagttg ctgacgcgca tcggcttcta ccggcacgcc tttggtccac
    51241 atttttaccg gggcattttc agtggtcagt aattcgtaat tcattttgtt tttctctttt
    51301 cgttgtttgc ggtcctgata attgcaaccg tcgtgccaga aaattaaata agcagattaa
    51361 ttttttaatt cattgtttta aaaagattat atctttacgt ccgtaaacgg agatttcccg
    51421 caaagccaat ttaccgataa tgaaatatcg tcttttataa ggatatctaa gatgcgtaaa
    51481 acagtggctt ttggctttgt cggaacagtg ctggattatg ccgggcgcgg cagtcagcgc
    51541 tggtcgaaat ggcgtccgtc actctgtata tgccagcaag aatcgttagt catcgatcga
    51601 ctggaattat tgcatgacgc ccgctcgcgc tcgctatttg aaacgcttaa acgcgatatc
    51661 gccagcgttt cgccagaaac cgaagtggtg agcgttgaga ttgaactgca taacccgtgg
    51721 gatttcgaag aggtttacgc ctgcctgcat gatttcgccc gtggttacga gtttcagcca
    51781 gaaaaagaag actatttaat tcacatcacc accggcaccc acgtcgcgca gatttgctgg
    51841 tttctgctgg ctgaagcgcg ttacctgccc gcccggttga tacaatcttc acctccgcgc
    51901 aaaaaagaac agccgcgcgg cgcaggtgaa gtgacgatta tcgatctcga tttaagcctt
    51961 tataacgcca tcgccagccg ctttgccgag gaacgccagc aaacgcttga ttttcttaag
    52021 tccggtattg ccacgcgtaa cccccacttc aaccgcatga ttgagcagat tgaaaaagta
    52081 gcgattaaat cccgcgcacc gatcctgcta aacggcccaa ccggtgcggg caagtcattt
    52141 ctggcgcgac gtatatttga attaaaacag gcgcggcatc agtttagcgg cgcgtttgtg
    52201 gaagtgaact gcgcaaccct gcgcggcgat accgccatgt cgacgctgtt tggtcatgtg
    52261 aaaggcgcgt ttaccggggc acgggaatct cgtgaaggat tattacgcag cgccaacggc
    52321 ggaatgttgt ttcttgatga gattggcgaa ctgggcgcag acgaacaggc aatgctgctg
    52381 aaagccattg aagaaaaaac cttttatccg tttggcagcg atcgccaggt gagcagtgat
    52441 tttcagctta tcgccggaac ggtgcgtgat ttgcgccagc tggttgccga tggcaaattc
    52501 cgcgaagatc tatacgcgcg gatcaatctc tggaccttca ccctgccggg gctacgccag
    52561 cgtcaggaag atattgaacc taacctggat tatgaagtgg agcgccacgc ttcactcacc
    52621 ggcgacagcg tgcgttttaa caccgaagcg cggcgcgcct ggctggcctt tgcgacatct
    52681 ccccaggcaa catggcgcgg taactttcgc gagctttctg ccagcgtcac gcgaatggca
    52741 acctttgcct ctagcggacg cattactctg gacgtggttg aagatgagat aaaccgtctg
    52801 cgctataact ggcaggagag ccgcccctcc gcgcttacgg cgttgctggg cgcagaggca
    52861 gaaaacatcg atctcttcga ccgtatgcaa ctggaacacg ttatcgcaat ctgccgccag
    52921 gcaaagtcgc tttccgcagc cggacgccag ctttttgacg tttcgcgcca gggcaaagcc
    52981 agcgtcaatg acgcggatcg gctacgcaaa tacctggcgc gttttggtct gacgtgggaa
    53041 gccgtgcagg atcagcacag ctccagttga atatggtggt ccgtcagcac ctgcatcacg
    53101 cttactggcg gcggggtgtc ggtgtagacg gcatctacca tgctgatgct acccatattg
    53161 accatcgcgt tacggccaaa tttcgagtga tcgacaacca gcataacgtg gcgcgagttc
    53221 tcaataatgg cgcgtttggt gcgaacttcg tggtaatcga actccagcag cgaaccgtcg
    53281 ctatcgatgc cgcttatccc cagaatgccg aaatcaaggc ggaactggga gataaaatcg
    53341 agcgtcgctt cgccaatgat cccgccatcg cggctgcgta attcgccacc ggcgagaata
    53401 atacggaaat cctcttttac catcagcgtg ttagcaacgt taagattgtt ggtgacaatg
    53461 cgcaaattgc tgtgattgag cagcgcgtgc gccaccgctt ccggcgtggt gccgatatcg
    53521 ataaacagcg tcgagccatt ggggatttgc tccgccactt tgcgggcgat gcgctctttt
    53581 tcttcggtct gggtggcttt acgatcgtgc cacggcgtgt taaccgaact ggaaggcagc
    53641 gccgcaccgc catgatggcg caggatcagg ttttgctccg ccagctcatt gaggtcgcgg
    53701 cgaatagtct gcgggctgac ggagaagtgc tccaccagct cctcggtact gacataaccc
    53761 tgctgtttaa ccagttcgat aataccgttg tgacgttgtg tttgtttcat ttctaaatcc
    53821 ctggaattat tttcgttttc gcgcattgag cgaatcaaca aaagccatcg ctaaacccac
    53881 ggctaacccg gcgatgtgtg ctccgttcgc catcgacatc ccaaacaaat caaaccatcc
    53941 ggcgacaatc cagatcagcg caaagataat taacccacgt tgcaggtaaa tgccactttg
    54001 cggatcgcgt tcgccacgta gccagacgta gcccatcagc gcatacacca cgccagaaag
    54061 cccgccaaac cacggcccgc tgaatttttg ctgcacatag ccgcttaaca gggcgctgat
    54121 aagcgtaatg acaattagct taccgctacc gaggcgtttt tccaccgcac cgccgagata
    54181 ccaccaccag agcaggttaa agaggatatg catcagcgag aagtgcatta acgcgtgggt
    54241 gaagtaacgc cagaactcaa atttcagtgc tggatcgaat ggccaagcca gccataacat
    54301 cacttcctga tcgccgagaa tttgcatggc aataaacacc accacgcagg cgatcatcat
    54361 cacccaggtc accggacctg cgcgttcacg caaggcggca aagaaaggat aacggcgata
    54421 atgcaggcca ctgccggtat ggcctgcctg ccagctcgcc gccagataac gcggatctgc
    54481 cgggttttcg agaaaacgcg ccagctccgc ccgtacgcgc tcggcctggg actcatccgc
    54541 cagccagaca tcgctttggt tatgttgttg aatcgtgagg ataacaccct gcgtcgccat
    54601 gtaatcaaca aacgcctgcg ccacgcgggg gttagcaaaa gaggtaatca tcaacatcgt
    54661 tgctgtcgct tattccacac aaaaggggac agtataaagc gttacgcgcc gtacgccacc
    54721 tctgcgggaa actgacgttg ccaggcttca aagccgccgt caatgctata gaccacatcg
    54781 tagccctgtt gcagcagata ctgcgccgcg cctttgctgc tattgccgtg ataacacatc
    54841 accatcaccg gagtgtcaaa gtcgttatta cgcataaaag cgcccagcgt gtcgttggtt
    54901 aaatggaaag cctgcaccgc atgtcccatt gcgaaactct gtggatcgcg aatatcgacc
    54961 agcaccgcct ctttttcctg caacttctgg tgcgcgtcgg caacgttaat acattcgaac
    55021 tgatccatgc gtctctcttt ctttacaaac aagtgggcaa atttaccgca cagtttacgt
    55081 cgaagcggca gataaacgcc ataatgttat acatatcact ctaaaatgtt ttttcaatgt
    55141 tacctaaagc gcgattcttt gctaatatgt tcgataacga acatttatga actttaacga
    55201 aagtgaatga gggcagcatg gaaaccaaag atctgattgt gataggtggc ggcatcaatg
    55261 gtgctggtat cgcggcagac gccgctggac gcggtttatc cgtgctgatg ctggaggcgc
    55321 aggatctcgc ttgcgcgacc tcttccgcca gttcaaaact cattcacggt ggcctgcgct
    55381 accttgagca ctatgaattc cgcctggtca gcgaggcgct ggctgaacgt gaagtgctgc
    55441 tgaaaatggc cccgcatatc gccttcccga tgcgttttcg cctgccacat cgtccgcatc
    55501 tgcgcccggc gtggatgatt cgcattggtc tgtttatgta cgatcatctg ggtaaacgca
    55561 ccagcctgcc gggatcaact ggtttgcgtt ttggcgcaaa ttcagtgtta aaaccggaaa
    55621 ttaagcgcgg attcgaatat tccgactgtt gggtagacga tgcccgtctg gtgctcgcca
    55681 acgcccagat ggtggtgcgt aaaggcggcg aagtgcttac tcgtactcgt gctacttctg
    55741 ctcgtcgcga aaacggcctg tggattgtgg aagcggaaga tatcgatacc ggcaaaaaat
    55801 atagctggca agcgcgcggc ctggttaacg ccaccggccc gtgggtgaaa cagttcttcg
    55861 acgacgggat gcatctgcct tcgccttatg gcattcgcct gatcaaaggc agccatattg
    55921 tggtgccgcg cgtgcatacc caaaagcagg cctacattct gcaaaacgaa gataaacgta
    55981 ttgtgttcgt gatcccgtgg atggacgagt tttccatcat cggcaccacc gatgtggaat
    56041 acaaaggcga tccgaaagca gtgaagattg aagagagtga aatcaattac ctgctgaaag
    56101 tgtataacgc gcactttaaa aaacagctta gccgtgacga tatcgtctgg acctactccg
    56161 gtgtgcgtcc gctgtgtgat gatgagtccg actcgccgca ggctattacc cgtgattaca
    56221 cccttgatat tcatgatgaa aatggcaaag caccgctgct gtcggtattc ggcggtaagc
    56281 tgaccaccta ccgaaaactg gcggaacatg cgctggaaaa actaacgccg tattatcagg
    56341 gtattggccc ggcatggacg aaagagagcg tgctaccggg tggcgccatc gagggcgacc
    56401 gcgacgatta tgccgctcgc ttacgccgcc gttatccgtt cctgaccgaa tcgctggcac
    56461 gtcattacgc gcgtacttat ggcagcaaca gcgagctgct gctcggcaat gcgggagcgg
    56521 taagcgatct tggggaagat ttcggtcatg agttctacga aacggagctg aaatatctgg
    56581 tcgaccacga gtgggtccgc cgcgccgacg atgccctgtg gcgtcgcaca aaacaaggca
    56641 tgtggctgaa tgcggatcaa caatctcgtg tgagtcagtg gctggtggaa tatacgcagc
    56701 agaagttgtc gctggcgtcg taaattaacg taaggtgatc aggtcagatt tcaatctggc
    56761 ctgagactga tgacaaacac aaaattgcct gatgcgctac gcttatcagg cctacgtggt
    56821 ttatgcaata tgttgaattt gcatgatttt gtaggcctga taaggcgttc acgccgcatc
    56881 aggcatgaac aaagcgccac gttgtcaaca atctgaccta ctttttgatt cagggaatta
    56941 ccgcaaagcc cacgccatca tacccaacgt aacaaaactt aagcacgttc cccactcact
    57001 ccacttccgt atctggcttc tatttttgct acgccactgg caataatatc ccaacgcaaa
    57061 accaagctgt gcacttatcg gaccgacaaa gtcgatccac tgttgagacc aaaatggcga
    57121 aaaattcagt gggagccaga caactgtcag aaagaaactg cgcatatttt ccagcaaagc
    57181 aatttgcgct ggcgcgacat aaaaaatgat attccacagg agcaaccaga aataaactgg
    57241 tgcaaatacc agccaacaat gcagtctgct tttcaattca ggtggcgttc tgcgcgcaaa
    57301 ttgccaacca acgcccacca ttgcacagaa gaaaagcgca tattcagccg ataaccatag
    57361 ttgtaattgc agaagggaaa tacgcctgtg catgagcggg attgagaaca gtatttctcc
    57421 cagaaaactc agcgtgatta cacaggcagc cagcgcaacc accgtcggcc acaccaacaa
    57481 cggtacaata acaaagccga gaaataacgg ataaatataa agtctgatta tattcatcat
    57541 tattgtcctg atgattcggg ggatgcttca tacagcggca cataccccac atcctctacc
    57601 aactgttgcc cctgctgact tataaaccag tcaacaaatt tctgcgtttc cggcgtggga
    57661 ttttcacgcg tcaccatata ggcatccaca atgtatggat aagtgccgtt gcggatattt
    57721 tcctcggtag gcgcaacgcc atttatagcc aaaagtttaa tctgcttgcg cattttgagc
    57781 atattgtcat tcatacgagt gacgtaatag tggaaggtgt acccaatgga agatggcgtg
    57841 ttttgatagt cggcaacttc agtaattaaa ccgcccataa gatcaatcac agtggatttt
    57901 ttagctggca gcattggggt gtcttgcata accaacccct tcataatcgt ttggcttcca
    57961 gaatcttcag ggcgctgcca gacttttata tgttcgtcac taccacctac tttattccag
    58021 cggctagttt tgccgctaaa aatatcttta acctggtgct cagagagaga atttaccggg
    58081 ttattaatat caacgataaa gacaaaggct tcgcgggcga aagcggtata tttcagttta
    58141 acacccgctt cttctgcacg ttttttctgt ccttccgacg gttgtgcaac aaaaataacc
    58201 gtcgcggaac ctttaatcag actatcgtag gcttcagctg ttcgggtttg ctgcaaacca
    58261 tacgcctgcc aggccagcga ggttcgatcc ttatcttttg gctcgggtac taatttataa
    58321 tacgcggagg catacagcgg gtataaggct gtagcgccgt acactctcgg ccatcttgac
    58381 caatcatcgc tgtaacgtaa tgcaggttgc ccccgcagag acgtcagtcc atggctctct
    58441 tcttccctga cgctgttata gtacccttcc cggtcgacac atcccgccag taacgccgtg
    58501 cctaatagta aaacaccggt gaatttattc atctcgctga ttcctttctc tcgagcgtta
    58561 ttccattgtt agatacagcg gcacatatcc cacatcttct accagactct gcccctgcgg
    58621 cgttaaaaac cattcgacca gtttttgtgt ttctgacgtt gtattttctc tcgtcaccat
    58681 aaatgcatcg acgatgtacg catatttgcc gttgcgaatg ttttccgccg tcggtgtaat
    58741 accgttaatc gctagcaatc ttatattttt atcagcattc atttgcgtcg cgtaatagcg
    58801 gaaggtatag cctattgcgt tgtttgtatt acggtattcg gcaacgactt taatcattcc
    58861 ctccatcacg cttgccactt ccgtttcctg cggcgagatc atgcggactt ttttcatgac
    58921 ctgtgattgc atcactgtct ggctgccaga gtcttccggg cgctgccagg tctggatctc
    58981 ctgatcgtta ccgccaacag tacgccaatt ggtaattgca ccactgaaaa tgtcacgcac
    59041 ctgttgttca gtcagggaat taaccggatt atccgcattg acgatgaaaa caaatgcttc
    59101 acgggcaaat ggcgtgtata gcaaagtgac gcccgattcc tcagcgcgtt ttttctgccc
    59161 gccggagggt tgcgccacaa aaataatatc ggcatcacct ttaacaatcc ggttatatgc
    59221 atcgggggta cgagagttct ccagatattc ccaaacgtga aaatcctctg gtattacact
    59281 taatgcataa aatgcagaag cataaattgg gtacgcagcc gtggcgccat cgattcgcgg
    59341 ccagttttgc ctaaactgaa tttgcggttt gccacgcagc ggcgtcagtc gattattgag
    59401 tttatcgggt cgccaggccc tgatatcaat attttcgcgt acaccgacac caggattttg
    59461 ctgcttatat aaatgggcct gatacccggc gacaatacca agtaagagaa gtagaaacag
    59521 gagtaagtta cggcaacgta gtccggacgt gccggataaa ggatatctgg tcggccaggc
    59581 gtatcccagc gaaaaactga tatgcgcccc aacgggaata atgaccatcc accagtactc
    59641 accacccact aatgagacta aaaaaacgcc caaccaggga agcataaggt ttgccaacca
    59701 accagctccc atctggcgaa attgataatc tgccagatgt agcgtgatta tccagccaac
    59761 cagggttaat agcgcgggta agagaaaaaa gcattgccgc cagacggcta tttctctgtt
    59821 ttctggcgcg ctatcaccgg cccacacgcg accagagaaa aatattatta tcgcccccag
    59881 cgtcagcata aagacgttga gtagaagata aattgttggc gtgataagga tattacaaac
    59941 ttcaataata ccggtaagcc cgacacccag catggcaaca accgctgcca aaaattgcgc
    60001 cagtatgggg atgccgaaaa aagtcattat cagcgaggtc aaaatccatt ttctatttgg
    60061 catcattctg tccattcttc ctgaatgacg gaattatact ccccgagaac cctcgccccc
    60121 tctggacact tttccgaaat aaaggcgaaa aaaacgggac cttgtggccc cgttctgttt
    60181 tttagcgaac ttacaatctc accggatcga tatgccagat atgatcagcg tactctttga
    60241 tagtacggtc agaagagaag tagcccatat tggcaatgtt cagcatcgct tttgcggtcc
    60301 actcttcctg aagctcgtag agttcatcga ctttatcctg acaatcgaca tagctgcgat
    60361 aatccgccag tacctggtag tgatcgccga agttgatcag cgaatcaacc agatcgcgat
    60421 agcgacccgg atcttccgga ctgaatacac cgctgccgat ttgcgtcagc acctgatgca
    60481 gctcctcatc tttctcgtag tattcacgcg gtttgtagcc ctgacgacgc agttcttcca
    60541 cttcttccgc tgtgttacca aaaataaaga tattgtcagc accgacatga tccagcatct
    60601 cgacattcgc accgtccaac gtaccgatag tcagcgcacc gttaagcgca aacttcatgt
    60661 tactagtgcc ggaagcttcc gtccctgcca gcgaaatctg ttcagacaga tctgccgccg
    60721 gaatgatcaa ctgcgccagg ctaacgctgt agttcgggat gaacacgact ttcagcttat
    60781 cgccaatctg cggatcgttg ttgatcactt tcgctacgtc attgatcaaa tggataatat
    60841 gcttcgccat gtaataggcc gaagccgcct taccgccaaa aatattcacg cgcggtaccc
    60901 acttcgcatc caggtcggcc ttgatgcggt tatagcgggt aatcacatgc aacacattca
    60961 tcaattgacg tttgtattcg tgaatacgtt tgatttgcac atcgaacaac gcctttggat
    61021 tcaccaccac attcagctgc tgggcgatat actccgccag acgcttttta ttctccagct
    61081 tcgcctgatg cacagcgtga ttaaccattg ggaaatcaca gtgttgttgc agctcattaa
    61141 gcaggctaag gtcggtgcgc cagttacggc ccaggtgttc gtccagcacg gctgaaagcg
    61201 atgggttcgc taccgccagc cagcgacgcg gcgtcacacc gttggtgacg ttggtgaaac
    61261 gacccgggaa gattttcgca aagtcggcaa acaacgattg caccatcaga ttagagtgca
    61321 gttccgatac accgttaact ttgtggctca caacaaccgc cagccaggcc atacgcacac
    61381 gacgaccgtt ggattcatca atgatcgacg cccgtcccag cagatcggta tcgttcggat
    61441 actgttcctg caaggttttc aggaaatagt cgttgatttc aaagatgatc tgcaggtgac
    61501 gcggcagaat tttacccagc atatcaaccg gccaggtttc cagcgcctcg ctcatcagcg
    61561 tgtggttagt gtaggagaag acctgacaac acacctcaaa cgcgtcgtcc cagctaaatt
    61621 ggtgctcatc gatcagcaga cgcatcatct caggaatcga cagtaccgga tgggtatcat
    61681 tgagatgaat cgcgatttta tccgccaggt tatcgtaggt tttatgcaac tgataatggc
    61741 ggcttaaaat gtcctgaatg gtcgaggaaa ccaggaagta ttcctgacgc aggcgcagct
    61801 cacgcccgga gtaggtggag tcatccggat acagtacgcg agatacgttc tcggagtggt
    61861 ttttatcttc cactgccgcg aagtagtcac cctggttgaa tttaccgagg ttaatttcgc
    61921 tactggcttg cgcactccac aaacgcagcg tgttggtcgc gtcggtgtcg taaccaggga
    61981 ttatctgatc gtaagcgact cccagaatct cttcggtttc aatccagcgc gtttttttac
    62041 cttcctgctg aatgcgacca ccaaaacgga ctttatagcg cgtgttgtgg cgtttgaatt
    62101 cccacgggtt accatattcc agccagtagt caggcgactc tttctggcta ccgttaacga
    62161 tgttctgctt gaacataccg tagtcatagc ggatgccgta accgcgcccc ggcaacccta
    62221 acgtcgccag agaatcgagg aagcaagccg ccagacgtcc caggccaccg ttaccgaggc
    62281 ccgggtcatt ttcttcatca atcagctctt cgagatttaa ccccatcgct tccagtgcgc
    62341 cctgtacatc ttcgtaaatt cctagcgaca acatggcgtt ggagagcgta cggccaatca
    62401 aaaactccat cgacaggtag taaacctgac gagtttcttg cgacaactgg gcacggtttg
    62461 aacgtaacca gcgctccacg agacgatcgc gcacagcaaa taacgttgcg ttcagccatt
    62521 catgtttatt ggcgacgacc gggtcctttc caatcgtaaa catcagcttg taagcgatag
    62581 agtgcttaag agcttctacg ctaagcgtgg gcgatgaata tgtaaacgga gcattcatat
    62641 aggcgtttcc tgaaaactat ttcaagcgat agtaaagctc acggtacgac ttcgccgcga
    62701 cctgccagct aaaatccatt gccatagcct gacgttgcac aaaccgccac agagaaggac
    62761 gggaccacag tacaaaagca cgtcgaatag cccgtaacag cgaccaggca ttactatctt
    62821 caaagacaaa cccactggcg acgccatctg caaggttctc aagagaacag tcagaaaccg
    62881 tatcagcaag cccaccggtg cgccgcacta acggcagcgt accgtacttc aatccataaa
    62941 gttgcgttaa gccgcacggt tcaaaacggc tgggcaccag aatgacgtcc gcaccgccca
    63001 taatgcgatg cgaaaatgct tcgtgatagc caatctgaac gcccacctgg ccggggtatt
    63061 ccgctgccgc cgcaaggaaa ccttcctgca gcaccggatc gcccgcgccg agtagcgcca
    63121 gctgcccgcc ctgctccaga agacccggta aggcctccag caccaggtcg aggcctttct
    63181 ggctggtcag acggctcacc actgcaaaaa gcggcacttt atcgtcaacc ttaagcccca
    63241 ttgcgatttg taactggcgc ttattttccg ctttatcttc caacgtatcg cgggtgtaac
    63301 gcgaggccaa tagtaagtcc gtctctggac tccagatttt ctcgtccacg ccgttcagta
    63361 cgccggaaag acgcccttca cgatgacgct gttgcaacaa accttccata ccgtaggcaa
    63421 actgcggttc ggtgatctcg cgagcgtagg ttggactgac cgccgtaatg tgatcggcat
    63481 agtacagacc ggccttcagg aaagagattt gtccgttgaa ttccagccca tgaatattaa
    63541 agaatgacca tggcaattgg atgtcattca tgtgatgtgc ataaaacatg ccttgatagg
    63601 ccaggttgtg cacagtaaac accgacttcg ccggacgccc gcgcgccgcc agatacgcag
    63661 gcgcaaggcc tgcatgccag tcgtgcgcat gcaccacatc aggacgccag aatgggtcaa
    63721 gcccgctggc catttctgcc ccaacccacc ccagcagcgc aaaacgcaat acgttgtcgg
    63781 tataggcaaa taagttggta tcgtgatacg ggcttcccgg acgatcatag agatgcggcg
    63841 cgtcaatcag gtatatgcca accccgttgt aatgaccgaa caacagcgtg atatgcccgg
    63901 cgaaggtatc acgacgggat actacctgcg catcggtcac gccacggcga atatcgggaa
    63961 atgcaggcaa cagtacgcga gcgtcaacgc cgtctgcgat ttgtgctgcg ggtaatgccc
    64021 caataacatc agccagaccg ccggttttaa gcagcgggaa catctctgaa catacatgta
    64081 aaacctgcat tatcgctcct gtttatgccc taacttccgt agcatttcgc gcgttaccag
    64141 cacgatgcct tcttctgaac gatagaaacg acgtgcatct tcctctgcgt tttcaccaat
    64201 caccatgcct tccggaataa cacaagcacg atcgatgacg cagcggcgca gacggcacga
    64261 gcgacctacc catacttccg gtaacaatac ggcggaatca atgttgcaga atgaattcac
    64321 gcgaacgcgc gagaacagaa cggactgcac caccaccgaa ccggagatca cacaaccgcc
    64381 ggaaaccagt gagttaaggg tcatcccgtg gctaccggag cgatcctgca cgaatttcgc
    64441 tggcggtaat gattcattgt aggtgcgaat tggccaattg cgatcgtaca tatccagttc
    64501 cggcaccaca gaggccagat cgaggttcgc tttccagtaa gcttccagcg tacccacatc
    64561 gcgccagtac ggctcggcat ccggatcgga ttgtacgcaa gagagcggga acgggtgcgc
    64621 ataggccaga ccggcttcgg tgatcttggg aatcaaatct ttgccaaagt cgtggctgga
    64681 gttctcatcg cgatcgtctt cttccagcag ttcatacaga tagtcggcgt caaagacgta
    64741 gatacccata ctcgccagag atttgctcgg atcgttcggc attgacggcg ggttagcagg
    64801 tttttcaacg aattcgataa ttttatcgtt ctcatcaacc gccataacgc caaatgcgga
    64861 ggcttcttca atcggtactg gcatacaagc aacggtgcaa cgtgcgcctt tttcgacgtg
    64921 atcgataagc atacgcgagt agtcttgctt gtagatatgg tcgcccgcca ggatcaccac
    64981 gtattccgct ttataacggc gaataatatc gaggttttgg gtgaccgcat ctgcggtgcc
    65041 gcgataccag ttttcccctt tcattctctg ctgtgctggc agcagatcga caaactcgtt
    65101 catttcttca ttgaagaatg accagccgcg ctgaatgtgc tgcaccagag tgtgggactg
    65161 gtactgggtg atcacgccca tacgacggat cccggagttg atgcagttag acagcgcaaa
    65221 gtcgataatg cggaacttac cgccgaagtg tacggccggt tttgctcgct tattggttaa
    65281 atccttcagg cgggtaccac gtcctcccgc cagtatcagg gcaacagatt tcaatggcag
    65341 ctggcgcgcc aacattaagt gatcgttctt ctctaaacta accatgacta actccttttt
    65401 tatcatctct ggaacacaca caatccgtgt gcaggtccct gccagacagc cgtaatcact
    65461 gggttatcct ctccagcgaa tgggggaatg gcgtgccact ccccagcagg taaaacaatc
    65521 tctgttacct caagcgtggc gttaattgcg atcaaaaagc gatccgagag cagaatttgc
    65581 agctgtttcg gcccgttttg ccactcatcc gtgcttaaag gttgagcata tcgatttagc
    65641 caacggacat tgccgtcgcc ttcttcccac cagcgattct ccaccaaagc gggaatacgc
    65701 ttgcgcagat ggattaacgc ggcggtaaat gcggttaaac cactgcttgc ctgcgaccag
    65761 tccaaccagg ttaattggtt atcctgacag taggcattgt tattgccatg ctggctgtga
    65821 ccatgttcgt caccggccag taacatcggc gtaccctggg agagcaacaa cgttgttaac
    65881 agggcgtgaa tgctgtcgcg ccgccgttca accaggtcaa gggtaccgcc taccccttct
    65941 ttaccatgat tgttactgta attgttgttg gtcccgtcgc gattttcttc tccgtttgct
    66001 tcattgtgtt tatggttgaa acaaacgcag tcgcgaagcg taaaaccgtc atgcgcggtg
    66061 acgagattaa tcgcggcact cggcagacga ccattacgtt taaaaacatc gctggaggca
    66121 gcaaaacgcc cggcaaacgc ccccagaggc aaatcataat gtagccagaa acgacgggca
    66181 gcatcgcgga aatgatcgtt ccactcggca aacagcggcg ggaaatttcc cacctgataa
    66241 ccaccaggag cgatatccca cggttcagca attaacttca cctgcgagag caccgggcag
    66301 ttctggatag cggtaaacaa cggcgcatcc tgacggaact ctggcgtacg gcccatgact
    66361 gccgccagat caaagcggaa accatcgacg tggcaggttt ctacccaata acgcaggcag
    66421 gcgctggcat aatccaccac cgccgggtga ctcaaattga gcgtgttgcc acaaccggtc
    66481 cagttgtgat aatcgccgtc ttctcttatc caataatagc tacggttgtc gatcccacgc
    66541 agcgagaata acgggccgtc gaggtccagt tccgcactat ggttgagcac gatatcaaga
    66601 atgacttcga tacccgcttt atgcagtgct ttgattgcat cgcgaaactc atgcagcgcc
    66661 gtttctggcg agcaggcata cgccggatgc agcgcaaaca tcgccaccgg gttgtaaccc
    66721 cagtagttac ttagccccat gcgttgcaga cgtggttcac tggcaaactg cgccactggc
    66781 agcagttcca gcgcggtaat gcccaattgt ttcaaatagt tgatcatcac cggatgcccg
    66841 agggctttat aagtgccacg gatctcgacc gggatctccg ggtgcaggta cgttaatcct
    66901 ttaacatggg cttcataaat gatggtgctg ccccacggcg tgcgcggcgg ggcatcatct
    66961 tcccagtcat agtgatcaac caccactacg catttcggcg caatggcggc gttgtcgcga
    67021 tagtcaggtt cattatgacc ggcgtgcagc agcgggttat ctttaaactc cccgtcaatt
    67081 tgccgcgcgc aaggatcaat caacaacttc gccgggttaa agcgatgccc ctcggcgggt
    67141 tgccaggggc catgaacgcg ataaccataa cgcaaacccg ggcgcgcatc cggcagataa
    67201 ccgtgccaaa tgtcgccact gtgccctggc aagtcatagc gatgttcctg gccattggcg
    67261 tcaaagacac acagttctac ccgctcggca tgagcggaga aaagtgtgaa gttgacgccc
    67321 tgaccgtcgt aatgcgcgcc gaggggagcg ggtttgccaa tggcgagttg tgtcattctg
    67381 cctcccgaac cagccagata gtggccagcg gtggtagcgt caggcttagt gaatgctgac
    67441 gaccgtggct ggcaatctca tcgctgtgta ccgtgccgcc attacctgca ttactgccgt
    67501 gatagtgcat ggaatcggta ttgaggatct cacgccattt gcctggctga ttaatgccga
    67561 agcgataatc atgacgcggc accggcgtaa agttactggc aacgatgatt tcgttaccct
    67621 ctttatcgcg acgcacaaag atcagcaccg agcgttcttt gtcatccacc accagccatt
    67681 caaagccgta cgggtcaaaa tccagttcat gcatcgcttt atggtggcgg taggtgaggt
    67741 tcagatcgcg caccagacgc tggacaccgt ggtgccagtt atcgccgcct tccaacagat
    67801 gccagtcgag gctggcgtca tggttccact cgcggccctg ggcaaattcg ttccccatga
    67861 acagcagttt cttgcccgga aatgcccaca tccagccata gtaagcgcgc aggttagcga
    67921 atttctgcca tgcgtcgccc ggcatgcggt cgagaatcga ttttttaccg tggaccactt
    67981 catcatgcga caacggcagg acgaagtttt cagtgtagtt gtagagcatc ccgaaggtca
    68041 gtttatcgtg atgatactga cgataaatcg ggtcgagctt catgtagtcc agggtgtcat
    68101 gcatccagcc gaggttccac ttgtaccaga agcccagacc gcccatatcc tgcggacgag
    68161 aaacgccagg gaaatcggta gactcctcag ccattgtcac cgcaccggaa acctgctcac
    68221 caagaatacg gttggtatta cgcaagaatt caatcgcttc aagattctcg cgcccgccaa
    68281 attcgttcgg gatccactcc ccctctttac ggctgtagtc acgataaatc atcgacgcca
    68341 ccgcatcgac gcgcagcgca tcaataccaa aacgttcaat ccagtaaagc gcgttaccga
    68401 cgaggaagtt actgacttca cggcgaccat agttgtaaat cagcgtgttc cagtcctgat
    68461 gatagccttc gcgcggatcg ctgtgttcat acaagttcgt accatcaaat tcggcaagcg
    68521 caaagtcatc agtcgggaag tggcctggca cccagtcgag aatcacgttc agaccagctg
    68581 cgtgtgcagc atcaatgaaa taacggaagt cgtcgcgagt accaaaacgg cgggttggtg
    68641 catacaggcc ggttggctga taaccccaac tgccatcgaa gggatgctcg ttaaggggca
    68701 gtagttcgag gtgggtaaag cccatccatt tagcataagg caccagttga tcggccagct
    68761 cgcggtagct caaccagaaa ttgttgtcgg tgtggcgacg ccaggagccc aggtgaactt
    68821 cataaataga gattggcgca tcaaactgat tcgctttttt gcgctcttca gtctgtacaa
    68881 ccttttccgg cagcccgcaa ataagagacg cggtttccgg gcgcatttgc gcttcgaagg
    68941 cataagggtc ggacttcaga cgcaagttgc cattggcatc aatcatctcg tatttataga
    69001 gctgaccgtt atgcgcccca gggataaaca gttcccagat gccgctctct ttacgcaggc
    69061 gcatcgggtg acggcgaccg tcccagtagt tgaattgccc aaccaccgag acccgacggg
    69121 cgtttggagc ccagacagag aaacgcgtac ctgtgacgcc atccatagta tctgcatgcg
    69181 cgcctaaagt ttcatacggg cgcaggtgag taccttcaga taatagccag gcatccattt
    69241 cctggattag cggaccaaaa cggtaaggat catcaatcag gttttgctgc ccatgccaga
    69301 caacagccaa ctgatagcgg aaaaaattct tacgtcgcgg aatgacgcca ctaaagaatc
    69361 cccgtgagtc gagacactcc agttttgcga gtttgcgccc ggttttcggt tcaatcaccc
    69421 acacatcggt agcgtcgggt aaaagggcac ggacttccag tcccgcggtg gttttatgca
    69481 ttcccagtac ggaaaaagga tccgcaaaat ggcctgcaat tagcgcgtta atcacgtctc
    69541 tatcgatacg atcggacatg cttgtcttcc tgttttattg tgtcacccca tccaattgga
    69601 tttttgactt ctggttgtga catttttttg acctgaacgg cgcagcactc tgtgcatcct
    69661 ctctgcgtcg tcctcacttc aggtaaggct gtgaatactc atgtattcag ccacccttaa
    69721 agaatagcca atgctctatt taactcccgg taaatcatga aacatctgcg cttactcctg
    69781 tattacgcac taacaggggc ggcatcgcgc cccagattta atgaataaag attacgccag
    69841 ttgacgaagc atccgacgca gcggctccgc ggccccccac agcagctggt cgcccacggt
    69901 aaaggctgac aggaactctg gtcccatatt cagcttacgc aggcggccta ccggcgtggt
    69961 cagcgtgccg gtaacggcag ctggggttag ctcacgcata gtgatttccc gatcgttcgg
    70021 aacgactttc gcccacggat tgtgcgcagc cagcagttct tccacggtcg gaatcgacac
    70081 atcttttttc aatttaatag tgaatgcctg gctgtggcag cgcaatgccc cgacacgcac
    70141 acataaacca tctaccggaa ttacggaaga tgtgttgagg atcttgttgg tttccgcctg
    70201 ccctttccac tcttcgcggc tctgaccgtt atcgagctgt ttgtcgatcc acggaatcag
    70261 gctacccgcc agcggcacgc caaagttatc caccggcagc tcaccgctac gggttaaggt
    70321 tgtgactttg cgttcgatat cgagaatagc agaggacggg gtcgcgagtt catctgctac
    70381 atggccatac agatggccca tctgggttaa taactcacgc atatgccgcg caccaccgcc
    70441 ggaagcggcc tggtaggttg caacggacac ccaatcaaca agatcattgg cgaataaacc
    70501 acccaacgac atcaacatca ggcttacggt acagttaccg ccaacaaaag tcctgatgcc
    70561 attatttaat ccgtcggtaa tgacgtcctg attgacgggg tcaagaatga tgatggcgtc
    70621 atctttcatg cgcagagacg atgctgcgtc aatccagtaa ccctgccatc cgctttcacg
    70681 aagctttgga tagatttcgt tggtataatc gccgccctga caggtcacaa tgatatcgag
    70741 ggcctttagc gcctccagat caaaggcatc ctgaagtgtg ccagtggttc cgccaaaaga
    70801 cggcgcagcc tgaccaagct gagaagtaga aaagaagaca gggcgaatgg cgtcgaagtc
    70861 gcgctcttca accatgcgtt gcatgagaac ggagccgacc ataccgcgcc agccgataaa
    70921 accaacattt ttcataagcg ttttttcctg caaagatgtg tgctgtataa atgtgccggt
    70981 ctcctcttgg cacatctttc accatacaaa aagcagccaa agtcgcaagt gaaattaatc
    71041 aatgatagcg aagccatcag taatgcgact tatcctgctt tgttagcacg cagaaagtcc
    71101 gcggcaatta tcagggaatt tgagttatga atgaaatcat ttctgcagca gttttattga
    71161 tcctgattat ggatccgctc ggaaacctac ctattttcat gtccgtactg aaacatactg
    71221 aaccgaaaag acggcgggca atcatggtgc gagagttgct tattgctctc ctggtgatgc
    71281 tggtgttcct gtttgcgggt gagaaaattc tggcatttct cagcctgcga gcagaaaccg
    71341 tctccatttc tggcggcatc attctgtttc taattgccat taagatgatt ttccccagcg
    71401 cttcaggaaa tagcagcggg cttccggcag gtgaagagcc atttatcgtg ccgctggcaa
    71461 ttccgctggt cgccgggcca actattctcg ccacgctgat gttgttgtct catcagtacc
    71521 cgaatcagat gggacatctg gtgattgctc tgctgctggc ctggggcggc acctttgtca
    71581 tcctgctaca gtcttcgcta tttttacgtc tgctgggcga gaaaggggtg aacgcacttg
    71641 aacgcctgat gggattgatt ctggtgatga tggcaaccca gatgttcctc gacggcattc
    71701 gaatgtggat gaaggggtaa ggttcaaaca ggtgctgcac acaaccagac gcggcacctg
    71761 ttttattacc tgaactgata atgaccttcg cccaatcgga taacccttcc ctcttcttct
    71821 gcgcggttca gccacacact aatttgttcg atggttaaac cagtacaaga cgccagcctt
    71881 tcaatactta tcgattcctt tgcaaggatc gccagttccg ccacaaagag ttgataaaaa
    71941 tcgacaggcg tatacgcggg cattactgcg taatttgcgt catcttcatg cagcgaaaac
    72001 agttctggtt gagcgttacg gagtgctctg tcatacatca gcccaggaac gtctggcagt
    72061 ctgatcgtca cattctcagc ctgttcagca ctccatgacg ccccattttc tactaatcgt
    72121 gaattggctg attgcatatc ctgatttggt ttaacctgca caggcagcca ctgatgtttt
    72181 aatgcctcca tcgccccggt tatcgttcca ccggtcattc ccgcacgtac aaccattgcg
    72241 ctttcagcaa ggcaataaat atatttattt cgcgccatcg cattgccgac ggtgaaacgt
    72301 acctctgggt aaaacggcga aatcaacacc aggttgcctg ctataagccc ttcacgccat
    72361 ttcactaacg tactcgtttt taacaagcta tcagctaata cgccaacggc agttcccccg
    72421 gcctccagtg ccgacgccat tgcacattca tcaatacctc gcgcaccacc agagataacg
    72481 caaatcccct gttgggccag tttcgcggcc agttgttggg tatagcgcaa atcgtcagtc
    72541 ggagcatctc gcgagccaac aatcgccata ccttccgctt gcagtaatgc tttattcccg
    72601 cagccaaata aaacgggagg tgcatccgtt cgcaatcggt ttttcaagcg aacgggataa
    72661 tcagcatctc cgcgggttaa gatccataaa ccggcgcgtt gccacttatc taccgccaac
    72721 gccagactat gaccacgcgc cagcaagcca agaatacgct cctgagaaat acgcggatcg
    72781 ctccagcctg taagtttctc ttgcggttgc ggcaccagaa gctcggcggg actgatacgt
    72841 tggtgcttca gccataatgc aaacttcccc cattcactat tactaagagg tttatactta
    72901 ctcgccgccg cgcgagaaaa atcgctggtt agcaggagag ttgcttgtgc attggctgaa
    72961 agattcatca attttttacc gaggtagaag caagggcaag aggataaacc gtcgggcaac
    73021 ctgcctggcg taacagtgct gttagaactg tcagcgtcca tgcagaatcc acgatatcgt
    73081 caaccagcaa cgccggacca ggcatcaaag gaggggtaat cacaaacgcc ccgtcgagat
    73141 tttgacactg gtggaaacgg ttttgctgca ttttctgcgg tggattgtcc acgacttttt
    73201 caatggcatc aataaaaggt aagccaagtt tcgccgccag tcgccgggca aaatcaggaa
    73261 ccaggtcgag gtgctttcaa aatcaggaac caggtcgagg tgctttaatg aaggaacgca
    73321 gcatacccag gttggttcag gatgcggatt ccagcgttgg cgaaccattt ccgcacatgc
    73381 ctccaccagt tcatcactga atcgccccgt ttttttctcc tgtgctacct gctttcccca
    73441 cccggaatat ccccaacgag aaaggattcg ccccgtagat ccttgtaaac tcgcaggcaa
    73501 gttcccttta aatccgtatt gcgtaaaagc cccagctgcc acctgtttat tgagatttaa
    73561 tgacaggtca gcgtgtttaa taaataagtt tgccgcatgg agtaagccgc tatcaatgtc
    73621 gggcgacaac aacggatgct gaagacaact gctacattta ccacaatgtt cggcacctgg
    73681 ctcatcgagt gcgcggcgca gaaattgcat tttgcactcc ttggtcgtga tatagctttc
    73741 aacgtctgcc agctcacttt ttctctgatt catcaaatgg ttaattcgtt catggtcagg
    73801 agaaaaactg acaattgttc tgcgccataa tttctcggta tacaccacag gcgatggatt
    73861 ttcagctacc aataatttca gtgctttttc tatttgccca taacggagat tcgtccgttg
    73921 ttcaatgcct cgtaaggtaa gaccgtcatt cacgctaagt acgttgagta tttcatgaat
    73981 ttgcgcctcc gcaggaaagg cgctttcacg gaagaattta tgaatagcac ggtcttcact
    74041 gccgcaaagc agtatgccaa cagcagaatc gataccgcga ccggcacgcc ccacctgtta
    74101 gtagtaacca actatcgaac ctggcatctg gtaatgaata acaaacccca aatccggttt
    74161 gtcaaagccc attcccagcg ccgttgtcgc aaccagcact ttaattttat ttgccagcaa
    74221 cgcctgctcc agatattccc tggcagtatt agaatcttcc gcgccttcac aagtcacgcc
    74281 gctgtagtaa gcaaatgcac tgataccatt tttccttaac cactcggcga caagttcggc
    74341 atcgcgagta gtcagggtat aaacaattcc cgatttggaa aactgaggga taaccgttgc
    74401 taaccatgcc agacgggatg agtgttcacc taagactaag gcatcaagcg ccaaactttc
    74461 gcgagccagc gttccacgct gaatcacaat gtcacccaat tgctgacgga tatcctcaac
    74521 gacacggtta ttcgctgtcg cggttgtacc cagaacaggg gtattcgcag gtagttgcgc
    74581 aaaatatcta atatacgtcg gtaatccggg cgaaaatcgt ggccccaatc agagatgcag
    74641 tgcgcctcgt cgaccacaag caagccaata tgatcggcta taggatataa aaccgtttcg
    74701 ataaagtcct ggtttgccaa acgttccggg gaaaccagca agcaatcgac ctcaccttgc
    74761 agcaacttat cgctaatgcg ctgccattct tctctgtttg tagaattcaa cgtttcagcc
    74821 gtaataccta accgttcagc ggcggcaacc tggttacgca tcagggcaag caaaggagag
    74881 atgataattg tcgggccagc gccgcgatcg cggaagattt tactggcgat gaagtacaca
    74941 gcacttttcc cccagccagt gcgttggaca accagcagtt ttcgccgttg gttcaccacc
    75001 gcgtctatgg cttcccattg cccttccctg aaagtagcgg tggtatttcc taacatccgc
    75061 tgtaaaagca attcagctgc gtgcttttcc atctttatct cctgcgatct ttaggcagtg
    75121 attagaacgg attatttgca ggtaaatttg cgagagagat caaaaagatg aagagaggag
    75181 aaaagagaaa tgaattactg cgagcaagct cgcagtaatt tgtcgcagta attaactcaa
    75241 cagctggaac gcaatcatcc caacgatggc accgacagtg ccgaggatgg tttccatcat
    75301 ggtccaggtt ttcagcgttt cggcttcggt cgcgccggta aatttaccga acaaccagaa
    75361 accagcgtcg ttaacgtggc tgacaacaat cgaaccaccg gcgatacaaa tcgacagcgc
    75421 cgccatttgc gcaccggagt agttcagttg ttcaataacc ggcatcacca gtcccaccgc
    75481 cgttaaacag gcaacggttg cagaaccctg aatgatgcgc actgcagcgg ccagcacgaa
    75541 gcaggtgatg gcaatcggca ggcccatgcc ggttaacgct tcgcccagtg ccggacctac
    75601 gccagagtca accagcacct gtttgaacac gccgcccgca ccaatcacca gcagaatgat
    75661 ccccgccggt tgcagcgcgt gaccgcaaat ctccatcact ttgtcttttg gcatgccctg
    75721 acgcattgcc aggccataaa tcgctaccag acaagcaacc agaatcgcgg taaacggatg
    75781 accaataaac tcgaaccatt cgtaagcggt agagccttcc ggcacaaaac gcgcggcaat
    75841 ggttttcagc cctaccagca ccagcggcag caggatcagc gacaggctga atccgaagga
    75901 tggcattttg ccttcgccga gatgcggttc gctgatgtcg tcaggaatat gcagctcaac
    75961 gtaacggctg atgaagttac cccacagcgg cccggcaata atcattcccg gaattgccgc
    76021 acacaggcca atcaggatca tccagccaaa atcggcgttc atctgcgatg ccagcagcat
    76081 tggcgctggc cctggcacca ggaacgccgc cgcggccgcc acgcctgcga ataatgggat
    76141 taccagcttc accaggttcg taccggtgtg gcgcgccatt gagaaagcaa cgctaatcag
    76201 cagaacaatc gccacttcaa agaacagcgg tagcgcacag accagccccg caaggccgat
    76261 ggcataatgc gcgcggctgt gaccgaagga tttaagcatt ttgacggcaa tctgatcgac
    76321 tgcgccggtt tcatgtaaga tcttgccaaa catagctccc agggcgacaa ccaccgccag
    76381 gaagccgagg gtgcctccca tccctttttc catcgtcgct gcgattttat cgagcggcat
    76441 accggaaaaa aggccagccc ccatggacac caccattaaa gccaggaaag cgtgcatacg
    76501 cgccttcatg actaaaaaca gcagcagtaa aacagaccct actgctgtta aaacaagcgt
    76561 taatgtagtc actacttatt tgcctttttt aataacctca atggtgcttg ccacaacacc
    76621 ttccagcggt tgatcgatat ccaccaccag tacatcggtt tcgtccgcac ccggctcctg
    76681 cagcgtttca aactgcgtca ccaacatttg ggttttaaag aaatggcctt tgcgcgcttt
    76741 caggcggctt tcaatcacat caaaatcgcc tttcaggtag atgaaagaga gattcggatt
    76801 accttcacgc agcaagtcac gatagtgttt tttcaatgca gaacagacga tcagcgacac
    76861 tttattagtg cgctgcatag caaacgcggc gtcgttcagc gcctgcaacc acggtttgcg
    76921 atcgtcgtca ttcagcggtt cgccagacgc cattttttcg atattgcgcc gtggatggag
    76981 gaaatcgcca tcaagaaacg cggcatgaag ttgatgcgcc acttcacttg cgaccgcaga
    77041 tttgccgctg cccgatacgc ccatcaagac gtaaatgtgg tgatcatggt tagtcgtgct
    77101 caaagtggtg cccccacaat acaagaatta aaaatgttac gggtaactgt tatcggtaac
    77161 attgtccagc cggacaatat cagaagcaat atccatccgt gccttaagtg tataagtgtg
    77221 agctacttca aatttgtggg cttaaataga tccgcccggt gacaaggtga aacctaaatc
    77281 taacattttc ggtgtcacag attcgccacg aatacgcgcc agcaggcgtt cagcgccaat
    77341 actgcccatc cgctcacgcg gcgtcagcac gctcgcaagt cgtggctcca tcacctgacc
    77401 aatgtcatga ccgtggaaac cggcaatcgc catatcgtca ggaactttta accccagacg
    77461 ctgacattca aacgccgcgc cgaccgccag atcatcatta gtacagaaca cgccatccag
    77521 ctgcggatat tcccgccgtg cctggcgaat cagttcaata ccggaagagt aagaagaaga
    77581 ttgctcaacc atcacgctat atggcaccag gcctgcatcc agcatcgcct gttcgtatcc
    77641 cttctgtttg atgatagtac gttcgtcgag acgtgcgccg agataggcaa tgtggcgatg
    77701 cccgcgagca ataatggctg tggtcatctg gcgtgccgct tcaaagttat caaaaccgac
    77761 ggcgatatcg agacacggcg actggctgtc catcaactcc accaccggga tccccgccac
    77821 ttcaatcatc tttaaggtgc gcggcgtgtg ggtacgttcg gtgaggatca gtccgtcgat
    77881 attccatgag agcattgatt cgagacgttc ttgctccatt tccggtttat aaccgtagtg
    77941 ggccagcatg gtctgataac cgtgcgcgtc ggtgacgctt tcgattccgc gtaatacttc
    78001 cgcgaaaacc tggttggtga gagaaggtaa caggacgcca atcgcccggc tggtggcgtt
    78061 agagaggata tcgggcgcac gattgggaat atagcccagt tcatcaagag ccgcggcaat
    78121 cttgccgcgt agagcgacgg aaacctgctc cgggttgcgt aaaaaacggc tgaccgtcat
    78181 tttggtcacg cctacacggt cagccacatc ctgaagtacg ggtcttttct ttttcatcgt
    78241 cctgaaggta caaaagagat agattctctt agtttaacac ggacgcatca gaaccttccc
    78301 ggggaaaaca gtgcttgttt acaattatta gatatacact aaatcaattg catcgcattg
    78361 tgctaacgac gctgcaaaac cccggaagcg tacatcagca cgcgaccgga gcgagcgagg
    78421 aagcctgcaa aaaggcaggc ttcaaagatg ccgttgagct ttaaaccggc ggcagatcga
    78481 acagtaacac ttcgctgtcg ctatccgcat ggatggagat tgcctgctca tcccagattg
    78541 ccagaccatc gctgatcgag gctttcacgc cgttaatggt gacattgcct ttcaccacct
    78601 ggatccagac gcggcgttca gcggcaatct gatgcaccga ctgctcatct ttcagcaacg
    78661 cccagcggta cagttccata tcctgatgca ctttcaatga accatcacgc gcatctggcg
    78721 aaagcaccag ttgtttgccc tgcactgcat cgaagcgacg ctgttcataa cgcggcgtaa
    78781 tgccgttttc ttccggcatg atccagatct gatacaggtg cagacgctcg gtgctgcttg
    78841 ggttgtactc tgagtgacga atacccgtac ccgcactcat aatctggaac tcgcccgccg
    78901 gaacctgctc tttattgccc atgctgtcct ggtgctcaac agtaccttcc agcacgtagg
    78961 tcaaaatttc catatcttta tgcgggtgag tgccgaagcc ctgccctgct tcaatcacgt
    79021 cgtcgttaat cacgcgcagc gcggagaagc ccataaagtt cggatcgtaa tagttggcaa
    79081 aagagaaagt atgccaggag tccagccagc catgatttgc atgaccacgt tcatttgctt
    79141 tgcgtaagta gatcatttgt attcgccccc tgaatgattt cgatgggctt agtgtggacc
    79201 cgatctgcct gggatgatag agggtgaaaa ttgacccctc tgttcaaaaa aattgaacta
    79261 ttcgaggggc catcaaactt acttcgcgag agtcactgtg gagggagagg cttgctcgaa
    79321 tccacgttca agtatttcca gattcgtaag aacttcagat tccttgacgt aatttggcgc
    79381 accgtgggtg atggtttgat acaacgcatc ataaacgcgc ccgtaatcgc ccatctccgg
    79441 cttcatctct tctctgaccg tcacgccctc gtcattgaca tactccagca caccgaccga
    79501 atcatccgct gcgaatcccg gttcgcccgg cataatatta gccttcaggc tggtttcctg
    79561 ctggtcgata ccatatttaa taaacgaacc tttcttaccg tgaacgataa atttcggata
    79621 atcgattttc accagatggc tggttttgac gatggctttt agatcgccat aaaacagttg
    79681 cgcttcgaaa gtatcgtcag gattggcttt attgcgcagg ctgcggatgt cataagcgac
    79741 gtgatccggg cgaccgaaca gagaaataat ctggtccatc gtatgcacac caaggccata
    79801 gaacgcgcca tcctgcggca gcccaggttt ggtttctgcc accgggcggt aatagtcaaa
    79861 atggctttcc acttcgacaa tctctcccag cttgccgctt tcaatcgctt ttttcgccgt
    79921 caggaagcag gaatcaaagc gacgattctg atatggcgtg acggtcagcc ctttgctttt
    79981 cgccagtgca aacagctctt tcgcctgcgc aagtgtcgga gtgaacggtt tttcgaccag
    80041 cacatttttc ccggcttcca tcgcgcgctt cgcgtactcg aagtggctgt ccgcatgggt
    80101 gcagacaaca accagcttaa catcgggatc gtttagcact tcgttgagat cgctggtgaa
    80161 atggatatga gagtaaatgg gagcttgttc ttccggcttt gcatggcggc gaaaaatatg
    80221 cgcgacatgc cagctatcct tgcggttaag tacatacggc agatggtaac gggtggtgct
    80281 tttgccgaag ccaataaagg cgcagttgat gaccatgatt tcgtcctttt taaggtggtt
    80341 atttacacct tagcgcaaag cggacgtggt tcctactgga gtgcgcataa cgaacacaag
    80401 cacttcccga ggataaattg agaacgaaag atcaaaaaaa aagccagcac ccggctggct
    80461 aagtaatact ggaagcaatg tgagcaatgt cgtgctttca ggttctccgc gagggtcttc
    80521 ctgatcgcga gacaataata atcattctca ttcgcacttg tccaacactt tttgcaaaaa
    80581 aatgcatttg actcgcattc gaaagtcaat gatgttgaaa gggacattta ccccaaagag
    80641 gacaaaggaa tgagtgagat agtgatacgc catgctgaaa cacgggatta cgaggccatc
    80701 aggcagattc acgcccagcc ggaggtgtat tacaacacac tacaggtgcc tcatccttcc
    80761 gatcatatgt ggcaggagcg actcgccgat cgtcccggca tcaagcaact cgtcgcctgt
    80821 attgatggag acgtcgtggg ccatctcacc attgacgtgc aacagcgccc acgccgcagt
    80881 catgttgccg attttggtat ctgtgtcgac gcccgctgga agaatcgcgg cgtcgccagc
    80941 gccctgatgc gagagatgat tgaaatgtgc gacaactggt tgcgggtaga tcgtattgag
    81001 ctaacggtgt tcgtcgataa cgcgccagca attaaggtct ataaaaaatt cggctttgag
    81061 attgaaggga ctggcaagaa gtacgcatta cgtaatggtg aatatgttga tgcgtatttt
    81121 atggcgcggg tgaagtaaag aatattctca aattgcctgt taccaatgtg aataattagt
    81181 tttgttcatc taatttgagt ttttccttat attaccacca tcattttgaa acaatttgta
    81241 acatcaagag acctttgaat cattactcgt taatatttct tattaaagag aacatatttt
    81301 cgcaagcaat agttattact gtttgtttat ggatagagag tctacttaat gggtacagca
    81361 gctataagtc atttacgata tgatcttaac aaatatgccc tttctttacg caaaaccgca
    81421 acacttgctt caacattttt tattgagtcc ccattagtta gattcgagta tctgcaagaa
    81481 atagaaaata
//
LOCUS       DBFIFM010000002        67968 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.002, whole genome shotgun sequence.
ACCESSION   DBFIFM010000002 DBFIFM010000000
VERSION     DBFIFM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.002"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(213..1112)
                     /gene="pgrR"
                     /locus_tag="N7C74_000069"
     CDS             complement(213..1112)
                     /gene="pgrR"
                     /locus_tag="N7C74_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HGU2430030.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLIIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            1250..2170
                     /locus_tag="N7C74_000070"
     CDS             1250..2170
                     /locus_tag="N7C74_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU2430031.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            2170..3100
                     /locus_tag="N7C74_000071"
                     /pseudo
     CDS             2170..3100
                     /locus_tag="N7C74_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            3121..3759
                     /locus_tag="N7C74_000072"
     CDS             3121..3759
                     /locus_tag="N7C74_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU2430032.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSPKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            4050..4778
                     /gene="mpaA"
                     /locus_tag="N7C74_000073"
     CDS             4050..4778
                     /gene="mpaA"
                     /locus_tag="N7C74_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HGU2430033.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(4753..5718)
                     /gene="ycjG"
                     /locus_tag="N7C74_000074"
     CDS             complement(4753..5718)
                     /gene="ycjG"
                     /locus_tag="N7C74_000074"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HGU2430034.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDTTLIVDANESWHAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            5837..6343
                     /gene="tpx"
                     /locus_tag="N7C74_000075"
     CDS             5837..6343
                     /gene="tpx"
                     /locus_tag="N7C74_000075"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HGU2430035.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(6387..7928)
                     /gene="tyrR"
                     /locus_tag="N7C74_000076"
     CDS             complement(6387..7928)
                     /gene="tyrR"
                     /locus_tag="N7C74_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HGU2430036.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(8076..9137)
                     /gene="ycjF"
                     /locus_tag="N7C74_000077"
     CDS             complement(8076..9137)
                     /gene="ycjF"
                     /locus_tag="N7C74_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HGU2430037.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(9134..10531)
                     /gene="ycjX"
                     /locus_tag="N7C74_000078"
     CDS             complement(9134..10531)
                     /gene="ycjX"
                     /locus_tag="N7C74_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HGU2430038.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRYKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            10686..11684
                     /gene="ycjW"
                     /locus_tag="N7C74_000079"
     CDS             10686..11684
                     /gene="ycjW"
                     /locus_tag="N7C74_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU2430039.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHTPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLEDHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGRQSCELLFRLIAGKPSPQNITVATHMTLK"
     gene            complement(11795..12700)
                     /gene="ompG"
                     /locus_tag="N7C74_000080"
     CDS             complement(11795..12700)
                     /gene="ompG"
                     /locus_tag="N7C74_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HGU2430040.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSISLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(12745..13827)
                     /locus_tag="N7C74_000081"
     CDS             complement(12745..13827)
                     /locus_tag="N7C74_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2430041.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGMRPEDIHPDAQEENNISAKISVVELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(13841..14500)
                     /gene="pgmB"
                     /locus_tag="N7C74_000082"
     CDS             complement(13841..14500)
                     /gene="pgmB"
                     /locus_tag="N7C74_000082"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HGU2430042.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERTQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            complement(14497..16764)
                     /gene="ycjT"
                     /locus_tag="N7C74_000083"
     CDS             complement(14497..16764)
                     /gene="ycjT"
                     /locus_tag="N7C74_000083"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HGU2430043.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSHEAWQRELDFASGELR
                     RNVVWRTSNGAGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEICAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRSGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMARYNVQQALNIARQFGCSDDA
                     FIHRAEMFLKELWMPETQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAVSCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(16761..17816)
                     /gene="ycjS"
                     /locus_tag="N7C74_000084"
     CDS             complement(16761..17816)
                     /gene="ycjS"
                     /locus_tag="N7C74_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU2430044.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGYPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLGSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(17826..18614)
                     /gene="ycjR"
                     /locus_tag="N7C74_000085"
     CDS             complement(17826..18614)
                     /gene="ycjR"
                     /locus_tag="N7C74_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HGU2430045.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KSAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYSVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLTWLRTC"
     gene            complement(18632..19684)
                     /gene="ycjQ"
                     /locus_tag="N7C74_000086"
     CDS             complement(18632..19684)
                     /gene="ycjQ"
                     /locus_tag="N7C74_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HGU2430046.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETIIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWKLLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(19715..20557)
                     /gene="ycjP"
                     /locus_tag="N7C74_000087"
     CDS             complement(19715..20557)
                     /gene="ycjP"
                     /locus_tag="N7C74_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGU2430047.1"
                     /translation="MATNKRTLSRIGFYRGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(20544..21425)
                     /gene="ycjO"
                     /locus_tag="N7C74_000088"
     CDS             complement(20544..21425)
                     /gene="ycjO"
                     /locus_tag="N7C74_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGU2430048.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVIFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(21446..22738)
                     /gene="ycjN"
                     /locus_tag="N7C74_000089"
                     /pseudo
     CDS             complement(21446..22738)
                     /gene="ycjN"
                     /locus_tag="N7C74_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(22752..24430)
                     /gene="ycjM"
                     /locus_tag="N7C74_000090"
                     /pseudo
     CDS             complement(22752..24430)
                     /gene="ycjM"
                     /locus_tag="N7C74_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            complement(24640..24954)
                     /gene="pspE"
                     /locus_tag="N7C74_000091"
     CDS             complement(24640..24954)
                     /gene="pspE"
                     /locus_tag="N7C74_000091"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HGU2430049.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATVVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(25029..25250)
                     /gene="pspD"
                     /locus_tag="N7C74_000092"
     CDS             complement(25029..25250)
                     /gene="pspD"
                     /locus_tag="N7C74_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HGU2430050.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(25259..25618)
                     /gene="pspC"
                     /locus_tag="N7C74_000093"
     CDS             complement(25259..25618)
                     /gene="pspC"
                     /locus_tag="N7C74_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HGU2430051.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(25618..25842)
                     /gene="pspB"
                     /locus_tag="N7C74_000094"
     CDS             complement(25618..25842)
                     /gene="pspB"
                     /locus_tag="N7C74_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HGU2430052.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(25896..26564)
                     /gene="pspA"
                     /locus_tag="N7C74_000095"
     CDS             complement(25896..26564)
                     /gene="pspA"
                     /locus_tag="N7C74_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HGU2430053.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            26731..27708
                     /gene="pspF"
                     /locus_tag="N7C74_000096"
     CDS             26731..27708
                     /gene="pspF"
                     /locus_tag="N7C74_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HGU2430054.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(27839..29104)
                     /gene="puuE"
                     /locus_tag="N7C74_000097"
     CDS             complement(27839..29104)
                     /gene="puuE"
                     /locus_tag="N7C74_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HGU2430055.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERAMQLGQRLTKTL
                     IDAKENVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALRD"
     gene            complement(29142..30422)
                     /gene="puuB"
                     /locus_tag="N7C74_000098"
     CDS             complement(29142..30422)
                     /gene="puuB"
                     /locus_tag="N7C74_000098"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HGU2430056.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLEMLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTATGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSDDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(30424..31911)
                     /gene="puuC"
                     /locus_tag="N7C74_000099"
     CDS             complement(30424..31911)
                     /gene="puuC"
                     /locus_tag="N7C74_000099"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HGU2430057.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLANIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLIEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIQEGESKGQLLLDGRNAGLAVAIGPTIIVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(32061..32618)
                     /gene="puuR"
                     /locus_tag="N7C74_000100"
     CDS             complement(32061..32618)
                     /gene="puuR"
                     /locus_tag="N7C74_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HGU2430058.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIILTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(32645..33409)
                     /gene="puuD"
                     /locus_tag="N7C74_000101"
     CDS             complement(32645..33409)
                     /gene="puuD"
                     /locus_tag="N7C74_000101"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HGU2430059.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPERDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            33621..35039
                     /gene="puuA"
                     /locus_tag="N7C74_000102"
     CDS             33621..35039
                     /gene="puuA"
                     /locus_tag="N7C74_000102"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HGU2430060.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            35188..35352
                     /locus_tag="N7C74_000103"
     CDS             35188..35352
                     /locus_tag="N7C74_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2430061.1"
                     /translation="MPMIKSPHGAGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            35342..36727
                     /gene="puuP"
                     /locus_tag="N7C74_000104"
     CDS             35342..36727
                     /gene="puuP"
                     /locus_tag="N7C74_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HGU2430062.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            36861..37106
                     /gene="ymjA"
                     /locus_tag="N7C74_000105"
     CDS             36861..37106
                     /gene="ymjA"
                     /locus_tag="N7C74_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HGU2430063.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            37419..39062
                     /gene="sapA"
                     /locus_tag="N7C74_000106"
     CDS             37419..39062
                     /gene="sapA"
                     /locus_tag="N7C74_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HGU2430064.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTAWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKLLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKTLSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            39059..40024
                     /gene="sapB"
                     /locus_tag="N7C74_000107"
     CDS             39059..40024
                     /gene="sapB"
                     /locus_tag="N7C74_000107"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HGU2430065.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            40011..40901
                     /gene="sapC"
                     /locus_tag="N7C74_000108"
     CDS             40011..40901
                     /gene="sapC"
                     /locus_tag="N7C74_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HGU2430066.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCM
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            40901..41893
                     /gene="sapD"
                     /locus_tag="N7C74_000109"
     CDS             40901..41893
                     /gene="sapD"
                     /locus_tag="N7C74_000109"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HGU2430067.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            41895..42701
                     /gene="sapF"
                     /locus_tag="N7C74_000110"
     CDS             41895..42701
                     /gene="sapF"
                     /locus_tag="N7C74_000110"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HGU2430068.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTNLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(42753..43916)
                     /locus_tag="N7C74_000111"
     CDS             complement(42753..43916)
                     /locus_tag="N7C74_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HGU2430069.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCVLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWTQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGFGGETLLKMSLAITVCYTLAFLVALTRIDNQK"
     gene            complement(43925..45298)
                     /locus_tag="N7C74_000112"
     CDS             complement(43925..45298)
                     /locus_tag="N7C74_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HGU2430070.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTETTAEADGTVSSFTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMTSAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLEENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFTPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQRAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQTGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(45302..48410)
                     /locus_tag="N7C74_000113"
                     /pseudo
     CDS             complement(45302..48410)
                     /locus_tag="N7C74_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            complement(48410..49531)
                     /locus_tag="N7C74_000114"
     CDS             complement(48410..49531)
                     /locus_tag="N7C74_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGU2430071.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTVKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVAAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHSGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(49680..50246)
                     /locus_tag="N7C74_000115"
     CDS             complement(49680..50246)
                     /locus_tag="N7C74_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGU2430072.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSEEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            50724..51512
                     /gene="fabI"
                     /locus_tag="N7C74_000116"
     CDS             50724..51512
                     /gene="fabI"
                     /locus_tag="N7C74_000116"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HGU2430073.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            51656..52783
                     /gene="yciW"
                     /locus_tag="N7C74_000117"
     CDS             51656..52783
                     /gene="yciW"
                     /locus_tag="N7C74_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HGU2430074.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LEKETLAPFLSWLDPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSTRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLAEILSLHPISLSLLERLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            52851..54785
                     /gene="rnb"
                     /locus_tag="N7C74_000118"
     CDS             52851..54785
                     /gene="rnb"
                     /locus_tag="N7C74_000118"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HGU2430075.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVRKDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(54904..54966)
                     /locus_tag="N7C74_000119"
                     /pseudo
     CDS             complement(54904..54966)
                     /locus_tag="N7C74_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            55020..57000
                     /gene="pdeR"
                     /locus_tag="N7C74_000120"
                     /pseudo
     CDS             55020..57000
                     /gene="pdeR"
                     /locus_tag="N7C74_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(56982..57110)
                     /gene="ymiD"
                     /locus_tag="N7C74_000121"
     CDS             complement(56982..57110)
                     /gene="ymiD"
                     /locus_tag="N7C74_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HGU2430076.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTEYSAKYIYSSTYARFR"
     gene            57148..57321
                     /gene="yciZ"
                     /locus_tag="N7C74_000122"
     CDS             57148..57321
                     /gene="yciZ"
                     /locus_tag="N7C74_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HGU2430077.1"
                     /translation="MSEFDAQRVAERIDIVLDILVADDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            57411..58160
                     /gene="yciT"
                     /locus_tag="N7C74_000123"
     CDS             57411..58160
                     /gene="yciT"
                     /locus_tag="N7C74_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HGU2430078.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            58429..58647
                     /gene="osmB"
                     /locus_tag="N7C74_000124"
     CDS             58429..58647
                     /gene="osmB"
                     /locus_tag="N7C74_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HGU2430079.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(58773..59099)
                     /gene="yciH"
                     /locus_tag="N7C74_000125"
     CDS             complement(58773..59099)
                     /gene="yciH"
                     /locus_tag="N7C74_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HGU2430080.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRSKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(59099..59836)
                     /gene="pyrF"
                     /locus_tag="N7C74_000126"
     CDS             complement(59099..59836)
                     /gene="pyrF"
                     /locus_tag="N7C74_000126"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HGU2430081.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(60029..61198)
                     /gene="lapB"
                     /locus_tag="N7C74_000127"
     CDS             complement(60029..61198)
                     /gene="lapB"
                     /locus_tag="N7C74_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HGU2430082.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(61205..61513)
                     /gene="lapA"
                     /locus_tag="N7C74_000128"
     CDS             complement(61205..61513)
                     /gene="lapA"
                     /locus_tag="N7C74_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HGU2430083.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLVRAERKIKRLENQLSPATDVAVVQHSSAAKE
                     "
     gene            complement(61662..62426)
                     /gene="pgpB"
                     /locus_tag="N7C74_000129"
     CDS             complement(61662..62426)
                     /gene="pgpB"
                     /locus_tag="N7C74_000129"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HGU2430084.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            62596..63186
                     /gene="ribA"
                     /locus_tag="N7C74_000130"
     CDS             62596..63186
                     /gene="ribA"
                     /locus_tag="N7C74_000130"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HGU2430085.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(63250..65925)
                     /gene="acnA"
                     /locus_tag="N7C74_000131"
     CDS             complement(63250..65925)
                     /gene="acnA"
                     /locus_tag="N7C74_000131"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HGU2430086.1"
                     /translation="MSSTLREASKDALQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDCEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLDLDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPIYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VARSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(66089..66184)
                     /gene="ymiC"
                     /locus_tag="N7C74_000132"
     CDS             complement(66089..66184)
                     /gene="ymiC"
                     /locus_tag="N7C74_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HGU2430087.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(66298..66465)
                     /gene="yciX"
                     /locus_tag="N7C74_000133"
     CDS             complement(66298..66465)
                     /gene="yciX"
                     /locus_tag="N7C74_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HGU2430088.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(66468..66608)
                     /gene="ymiA"
                     /locus_tag="N7C74_000134"
     CDS             complement(66468..66608)
                     /gene="ymiA"
                     /locus_tag="N7C74_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HGU2430089.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            complement(66927..67901)
                     /gene="cysB"
                     /locus_tag="N7C74_000135"
     CDS             complement(66927..67901)
                     /gene="cysB"
                     /locus_tag="N7C74_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HGU2430090.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT        16589 a        17969 c        16671 g        16739 t
ORIGIN      
        1 aaatcagatt tagtgtaaat aatagacgcc aaaatgcggc aaagcacata gaacaccgaa
       61 cactacgttc gggcttcaca cgttcaaacc agttttcggt atcataatca gcatctgtcg
      121 ccattgtacg aagaataaaa aataaccaaa acgttgaaag gaactgagaa tacgttaaga
      181 gaagaattaa attaatgtat cagcaagtag tgctaaacag cacctttata cttcagccta
      241 tctataaata atgagaatgc cgagccagca tgtcgacggt gtggatagta caggtgataa
      301 ccgggtaaat ctggtgtgaa tttatccaaa acacgtatca gttttttttc tttaatcgcc
      361 cgctcaacct gatcataagg tagatacgcc aatccatgcc catcaattgc agcatcaatg
      421 atcaggtcta tcgtattcag taaaagctga ccttccatgc gaacacgaac ttcacgtcca
      481 ccgcgtatta atctccagcg atttgctgta cccgatgtgg gaagatacaa attaattgcc
      541 tgatgatcta ttaattgtga cactgacgtt ggaacacttc ggcgagaaaa ataatccggt
      601 gagccaacaa tagccattgg tatatctggc ccgattcgaa tggcgatcat atctttatcc
      661 atctccccac ccagacggac gcctgcatca aaacgttcag aaacgacatc agtcaaacca
      721 taatcaatga taagctgaat atcaatttca gggtgtgatt tcaggaatgt gcgcatggct
      781 ggtaacaata tcgtttttgc tgcatgttct acagtagtaa tacgtattgt cccggatggg
      841 cggttctgca gatcgctcag ggatgccagg gcagaatcta tgtcatgcag catagggcca
      901 agaacagaca aaagatgctc gcccgcttca gtgggtacaa cactgcgcgt ggttcgcgtc
      961 agaagccgca atcccaatcg ttcttctata cggcgcacta tctggcttaa agctgactgc
     1021 gccatgctca ggcgggctgc tgcgcgagtg aagctacgct cctctgcaac gacgacaaac
     1081 gccatcagat cagcgatctc ttcacgcttc attcagtatc tccatacaat ctattaatca
     1141 tttaaatgat aggtctaatc atgatttagg gtctaatcaa cttaatgatc aatatctata
     1201 gtgatatcac caaccgttaa aacgaaccaa caactccatg aggaacggaa tgatgaataa
     1261 taaagtcagc ttcactaaca gcaataatcc aaccatctct ttgtctgcag tgatctattt
     1321 tcctccaaaa tttgatgaaa cccgtcaata tccggcaatt gtggtatctc acccaggtgg
     1381 gggcgttaaa gaacaaacgg ccgggaccta tgccgaaaaa ctggcggaaa agggatttgt
     1441 cactattgct tatgacgcat cttatcaggg tgaaagtggc ggcgagccgc gtcagttaga
     1501 aaacccatac attcgtactg aagacgtcag tgccgtgatt gattacctga caacgctttc
     1561 ttatgtcgac aatacccgaa ttggtgcgat gggaatttgt gccggtgcag gatatacagc
     1621 caatgctgca attcaggatc gccgcatcaa ggccatcggt accgttagtg cggtcaatat
     1681 tggttcgatg ttccgtaatg gttgggaaaa caatgtgaaa tctattgatg ccctgccata
     1741 cgttgaagcg ggttcaaacg ccagaaccag cgatattagc agcggagaat acgccattat
     1801 gccactggca ccaatgaagg agtccgatgc gccgaacgaa gaactgcgtc aggcctggga
     1861 gtattaccac acccctcgcg cgcagtatcc aacagcaccg ggttacgcta ctttgcgcag
     1921 ccttaaccag attattacct atgatgctta ccatatggcg gaagtgtacc tgactcagcc
     1981 gacgcagatt gtggcgggca gccaggcagg gagcaaatgg atgagtgacg acctgtacga
     2041 tcgggcatca agccaggata aacgctatca cattgttgaa ggcgcaaacc atatggatct
     2101 ttatgacggc aaagcctatg tggctgaagc aatttcagta ttagctccgt tctttgagga
     2161 aactctgtaa tgaaacatat tcaaatccgc aatagtgata tggcctggca tattgctgcc
     2221 aatattcagt ttccaccaaa ttttgatgag tcaaaacagt atccggcgat tatcagcgtc
     2281 catccttttg gcagttgtaa ggagcagacg tctggcaata tctatggcaa ggcattggct
     2341 gaaaaaggct atgtcgttct cgcttatgac gccagtttcc agggagaatc tggtggggag
     2401 cctcgttgga tagaagatcc gacacagcgc gtagaggata ttagccgtgt tatcgattat
     2461 gcagtaactc tgccatatgt tgatgctgaa cgcattggcg tgctgggtgt gtgtgggggg
     2521 gggtggctat gccattaatg ccgcgctgac agaaaaacga attaaagctg tagtgagtat
     2581 tacgggtgtt aatatcgggc gtctttttcg ggaaggtttc agcaattacg atccgattgg
     2641 cgcattgaat gcaatggcgt cgcaacgaac caaagaggct cgtggtggag aattacagat
     2701 caacgaatta cttcccgcca gcctggatgc agcaaaagcc catggactga ccgaacgcga
     2761 tgtttatgaa gcaactgact actataaaac accacgcggg caacaaccgg gaggtgcgac
     2821 aaaaatgttg ttctcacatg cacagaaaac gctggcctgg gatgccttcg catttactga
     2881 agtgttactg actcagccgg tgatggtagt agttggtgaa aaagtcggtg ccttcggtgc
     2941 ttatcgtgat ggactggaag tctatggccg ggcgatggtt tcgcaggatc gtcagttagt
     3001 ttcattaccc gatttctcac attatgaact gtatgacaaa ccggaagcag ttcaggaggc
     3061 gctggaaaaa gttattcctt ttttcaacac ccatctgtga taagtttcat gagaaatatt
     3121 atgaaaaatg tactcattct tggtgctggc ggtcagatag cccgccatgt gattaatcaa
     3181 ctggcagata aacaaaccat taaacaaact ttatttgctc gtcagccagc aaaaatacac
     3241 aaaccctatc caactaatag ccagattatt atgggggatg tactgaatca tgcagcactg
     3301 aagcaggcca tgcagggaca ggatattgtg tatgccaacc ttacggggga agatttagat
     3361 attcaggcaa attcagtgat cgctgcgatg aaagcctgtg atgttaaacg tctgatcttt
     3421 gttctttccc tcggcattta tgatgaagtt ccaggaaaat ttggcgaatg gaataatgca
     3481 gttattggtg agcccctaaa accattccgc agggctgcgg atgcaattga ggcttctgga
     3541 ctggaatata caattcttcg ccctgcctgg ctgacggatg aagatattat tgattatgag
     3601 cttacctcac gcaatgaacc attcaaaggt acgatcgtat cacctaagag tgtcgcagca
     3661 ttaattacag atatcatcga taaacctgag aaacacattg gtgagaatat aggcatcaat
     3721 caacccggga ctgatggcga caagccattc ttcatgtaaa tcaattatat aaccaccatc
     3781 gtactgtact atgtgcagta cgatttatta aacttattaa ggtgaactga tcaatcagat
     3841 gcgattaaca tgtgtagaac cagtaagaat aaaatacgcc atgtatataa cattacgaca
     3901 attctaatat ctgcacatca atgaaaatac aaacaacttc cggcaaaaaa gtgatacaag
     3961 atcgatgaca tagcgttgaa gaaaaggatt tgataatccg caaaatttgc acaacactgc
     4021 ctgtaatact tcctgaacca gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg
     4081 gcgcatttcc gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct
     4141 ggtttccggc ccctgcagcc agtcgcgaaa gtggtttgat tctggctggc actcacggtg
     4201 atgaaaactc ttcggtcgtc acgctctcct gtgctctgcg gactttgaca ccttctttgc
     4261 gccgccatca tgtggtgctg tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca
     4321 atgctaatgg tgtggattta aacagaaact ttccggcggc gaactggaag gaaggtgaaa
     4381 cggtttatcg ctggaacagc gccgctgaag aacgtgatgt cgtattactg acaggtgaca
     4441 aacctggctc cgaacctgaa acccaggcgc tgtgccagct tatacaccgc attcaacctg
     4501 cctgggtggt ctcattccac gatccactgg cctgtattga agatcccaga cacagcgaat
     4561 taggcgaatg gctggctcag gcgtttgaat tgcctctggt aaccagcgtc ggttatgaaa
     4621 cgcctggctc tttcggcagt tggtgtgcag atctgaactt gcattgcatc accgcagagt
     4681 ttccgcccat ctcctccgac gaagccagcg aaaaatatct ctttgcgatg gcaaacttgc
     4741 tgcgctggca tcctaaagat gcaattcgcc cgtcgtgaac tgaagcgccg gttccacatc
     4801 taccgccagc caggtcggtc cgtcaagatc ggcgaaactg acctgcggca ccagcggtaa
     4861 agcggcgcta atggcccgag aggtacacaa catgcagccc agcatcagac tgaaaccttg
     4921 tgcacgcgct tcagtcgcca gcgccagcgc ttccgtcaga cccccggttt tatcgagctt
     4981 aatattaacc atctcatagc gccctttcag cgccttcaaa ttgctacgag tatgacaact
     5041 ttcatcagca caaatcggca acggatgaat aaaattctcc agcgccgcat cgtcctgcgc
     5101 aggaagcggt tgttcaagca tcgcaacgcc taaatccgcc aatagctggc aacgtgccgc
     5161 caacccttct gcatgccagg attcatttgc atcaacgatc agcgtggtat cgggcacagc
     5221 tgtgcgaatt gccaccatcc gctcactgat aagatggtta tccagcttca ctttcagtaa
     5281 tttcgcgccg gcctgccaga gtgttgatgc actattggcc atctgatcag gcgtaccgat
     5341 gactaccgtc tgtgcagtga taactgtctc gggaagcgtt atgccgatca aatcagccag
     5401 cgattgctgc tgttttcgcg cggccagatc ccacaatgca caatccagcg cattacgtgc
     5461 tgcgccggca gggagaattt tttgcaactc ctcccgtgtc agccctttct ctaattgcgg
     5521 cacgacgctc ataatttgcg ccattaccga ggcatcactt tctccataac gcggatacgg
     5581 cgtgcattcg ccggtgcctt taataccctc ttcttccagt tcaaccacca ccacgcgcgc
     5641 ttcactgcga cttccccggg caatcacaaa cggggtatgt aagggccagg cttcctcaaa
     5701 taccttaacg gttctcatct ttactccttg gtgtcgcggt ggtctgcgga ttaagcgcaa
     5761 atagcgtttg ctgtgttatt gacagttagc ataaactagg tgtgacgtta actatatgta
     5821 aacaggaaag ataatcatgt cacaaaccgt tcatttccag ggcaacccgg ttacagtcgc
     5881 caattccatc ccgcaggcgg gtagcaaagc gcagactttt actctcgtgg caaaagatct
     5941 gtctgacgtc accctcggtc agtttgcggg taaacgcaaa gtgctgaaca ttttcccgag
     6001 tattgatacc ggtgtttgcg ccgcatcagt acgtaagttt aaccaactgg caaccgagat
     6061 cgacaacacc gttgtgctgt gtatctctgc cgatctgccg ttcgcccagt ctcgtttctg
     6121 cggcgcagaa ggtctgaaca acgttatcac cctctccact ttccgtaacg ctgaatttct
     6181 gcaagcctac ggtgtggcaa ttgctgatgg cccactgaaa ggtctggcag cgcgtgccgt
     6241 tgtggttatt gatgaaaatg acaatgtgat tttcagccag ctggtggatg aaatcaccac
     6301 cgagccggat tacgaagcag ctctggctgt actgaaagca taagttaata tgcctgatgg
     6361 tgttgcacca tcaggcatat tcgcgcttac tcttcgttct tcttctggct taagccatat
     6421 tcccgcaact tattggcaat cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc
     6481 gtgctgggat aattgcgata aagctgggtt aataccgagc gttcaaaacg gctggtgatt
     6541 tcgtccagcg aaccttccat cgcatcttcg cccacggcta ccgttgcggc gtcataatcc
     6601 ggcaacaaaa tatcctgtgg acgcagctca taaccgtcca gttgtgtcag tgcgcgatag
     6661 atagcgttct ttaactgccg cacatttccc ggccacgcat aacgcgtaag tacagtattc
     6721 aggtcagcgg ccagtttcgg acgcggcacg ccctgctcgt cggcaaagcg ggcgacgaac
     6781 agctcagtta acggcatgat gtcctgcgga cagtcacgta gcggcggcag gttgagcgtc
     6841 aacacgttca gacgataata gagatcttca cggaacatgc ctttttgcac cagttcgacc
     6901 agattcttct gcgtagcgca aatcacccgc acatcgacat gcacctcatg gtcttcgcca
     6961 acccgacgga aagtgccatc attaaggaaa cgcagtaatt tcgcctgcat ccgtggtgac
     7021 atctcaccta tttcatccaa cagcaccgaa ccaccgttcg cctgctcaaa gaatcctttc
     7081 ttcccttccg gagcatgacc aaacagctca ctctcgaccg catcttccgg tatagacgca
     7141 cagttcagcg ccaggtaagg tttgctcgct cttgggcttg cctgatggca ggcgtaggca
     7201 aaaagatctt tacctgtacc tgtgtcaccc gtaatcagca gcggcgcgct tagcatcgcc
     7261 agtttctgcg cctgttcgac aacatgcttc attttcgggc tgacagcgac aatttgactg
     7321 aaggcgctga cgtcctgggc ggcgacattt tgcaactgac ggcccatacg aatcgttgat
     7381 cgcaacatca ccaccgcacc tgtcaggacg tgttgatcat tttcatcctg aagataaaca
     7441 ggcgtaatct ccatcaggaa gttctgtcca ttaataacaa catgctcgtt atgggaatct
     7501 tgcggttcgc tttccagcca acgtaaaaaa ttaaagccgt taatcaattg tgcggcggta
     7561 tggttgcgca gtcgatccaa tttttgccca aaaagctgac agctcgccgg gttcgccata
     7621 tccactttgc ttttcatatc gacagagagc acaggttcag gcaacgcctc cagtaacgcg
     7681 ctcaacgcca gatgctcacg ttcggaaggc atccacggga cagtacgcac atcggtaaca
     7741 cctgcaatac ggcgtatttc ggccatcaga ctgctgaaac tctcaaactc cagttcagca
     7801 aaattgaggt agattcgccc aatgggatca atctcaatac cgcgtaaatc aatgcctctt
     7861 agcacgagta gatcgagtaa ttcgcgggtc agaccgagtc ggtcttcaca aaagacttcc
     7921 agacgcatgg gaaccttcac ctgaaaaaag aacaattaat atgatgatat gacactattt
     7981 gaatagcagg aaggtttctg tcaacaatca ttgacacaaa gacggaaaaa cgggcacttc
     8041 ggcgtaaaga tatccggtca gcacccgata ttgcattatt tttcgctggg cgttttgcct
     8101 ttttgcagcg tttctttcac ctgaccgata agctgacgac gaaaatcccc gaggcgaggt
     8161 ttgtcatcgt caatccacgg cagcgggcgg caaagctcca tagctttaat ccccagtcgt
     8221 gccgtcagaa gtcctgcacc aatcccctga gctgcgcggg tagacaaacg agcagcgaga
     8281 tcttgcgaca tccagtccat tcccacttcg cgcaccaatt cgctggctcc ggcgaaagcg
     8341 atattcagca ataccagctt gaacaggcgc aaacggctgt aatatcccag ttcaatgcca
     8401 tacagcgtgg cgatgcgatt aatcaaacgc agattgcgcc aggcgataaa tgccatatcc
     8461 accagcgcca gcgggctgac cgcaatcatc aacgttgatt cagctgctga gcggctgatt
     8521 tcgcgccgcg cctgggcatc taaaaccggc tggaccagat gagcatacag gctgaccact
     8581 tcacgatcgt tctgcgtttc atggattgag gcataccagc gttgcagcgc tggatgagac
     8641 tgatcaatac ccgcctgctg cgccagtttt tcgcaaaatg cgcggccttt gcccgtgccg
     8701 tggctgtgca acaaatcgcg cgcttcgtcg cgttcatggg cgcgctgtcg caagcgccat
     8761 aagcgccgcc actctgttac cacagaacct acgccagcgc cgataatcaa tgccccagca
     8821 gcacatccac ccagcgccac ccagtcctga gtttgccagg cattcattgt ccactgtaca
     8881 ccctgcccga caacgcttgc gccaaacaga gccagcccgc ccatcaccat tttgcgccac
     8941 aggctgcgtt tcggacgtaa cgctgcgtcc attaccgctt cgacttgccc ctcttcttcc
     9001 tgcgcttcgt cgagcgtggc cggggcaaaa ttttgcgcct gattttcgtc aaaggtctgc
     9061 tgcgccctga attttggatt ctgttcgacc tccagcggac cgtcgaaatc aatacgtggt
     9121 tttaacggtt cggtcatcgc aatttatctc ctattaaaaa ttccagcgcg gcatcaagac
     9181 gaatatgcgg cagcggtttg tcgacatcca tcacctgcgg gcgaaacgct tcaaactgga
     9241 acccttgctt atcccagaac gcctgaccag gcaaacgtgc gggaacttcg ccaggataaa
     9301 cagtgagcgg tgcgccatcg ctaagtcgat taccgcgcag cgccgggatt ttctcaccgt
     9361 taacatcaat aatgccactg gtggtcgcct gaactgacgc cagccccagg caatccatgc
     9421 tgatcccttc gaacgccgca ttttgccagg catcctgaat tagttgttgc agcaatgaaa
     9481 ccatattggc gtgctgatcg atggtcacat ggtccgcttt agtggcagca aacaatagct
     9541 tatcgataac cggcgaaaac aaacgcctga acagggtacg ctgcccatag tggaaacttt
     9601 gcatcagctg cgtcagcgcc agacgcatat cattaaatgc ctgtggccca ctgttgagag
     9661 gttgcaggca atccaccagc acaatctggc ggtcaaagcg cagaaaatga ttcttataga
     9721 accccttcac caccttctcg cagtaataat taaaccgctc gcgcagcatt ccggcattgg
     9781 tgtgcttatc ggcctgcgcc agtttggact cgccccaggc atcgacatcc ggccacggga
     9841 agaattgcag cgcgggcgca cctgccatat cccccggcaa gacaaagcgc ccaggctgaa
     9901 taaagtgcag cccctgctgt ttacagtggt ggagataatc ggtccacgcg gcggcaatgt
     9961 ctgccagccg gttttcgtcg gcaggcgcta gcgggtccag cccttcgcac atcattcgcc
    10021 atttcgccga ccattcgccg cgctgaccat tgagtaagcc cgtcatctgg cgcgaccagc
    10081 ttaaatagtc ctgcgccagc atcggcaggt cgagcaacca ttcgccaggg taatccacaa
    10141 tctccagata cagcgtggag gtatccttaa agtggcgcag cagcgaatcg ttcgatttat
    10201 agcgtagtgc caggcgaatt tcgctgacgc cgcgcgttgg cgtcggccag gcgggaggat
    10261 cgccatacag ctgcgccaac ccttcgtcgt aggtaaaacg cggaatgcca aagtcacgct
    10321 gaggaatgcg ttttacgccc agcagacgct cttcacgcac cgcacttaat agcggcaaac
    10381 gtgctccggc atgaatattg agcaactgat tgaccatcgc agtgataaac gccgttttgc
    10441 cgctgcggct aagtccggtt acggcgaggc gcagatgtct gtcgacaccc cgattcacca
    10501 gcgcattaag ttcattttta agtcgcttca ttgctgtcct gtgctgctct gaagaccaga
    10561 aaaaaatcat tcataaggat atgaatgtgg gggccaggcc attgattatc aactacccgt
    10621 gttgtcatcg acgggcgaac tccataagat gtgcagcatt cacgttcgga cggagttcct
    10681 gacctatgtc ccctactatt tatgatattg ccagggttgc aggcgtatca aaatccaccg
    10741 tatcacgcgt gctgaataag caaaccaata tctccccgga agcgcgcgaa aaagtgttac
    10801 gggccattga agaattacag tatcaaccaa acaagctggc ccgcgcgctg acctcttcgg
    10861 gttttgatgc cattatggtg atttctaccc gttcgaccaa aactacggcg ggtaatccgt
    10921 ttttctctga agttttgcat gccatcaccg ccaaagctga agaagaaggt ttcgacgtga
    10981 tattgcagac gtcgcacacc ccggcagaag acttacaaaa atgtgaaagc aaaattaagc
    11041 agaaaatgat taaaggcatt attatgctaa gttcgccagc ggatgagtca ttttttgccc
    11101 aacttgataa atacgatatt cctgtggtgg tgattggcaa agttgaaggt caatatgccc
    11161 atgtttattc tgtcgatacc gataattttg gtgacagcat tgcgttgacc gatgcgttaa
    11221 ttgaaagtgg acatcaaaat attgcctgtc tgcatgcacc gcttgatgtc catgtttcag
    11281 tggatcgggt aaatggttat aagcagagtc tggaagatca taatattgca gtgcgtgatg
    11341 aatggattgt tgatggcggt tatacccatg aaacagcact gcaagcagca cggcaattat
    11401 taagtcagtc accgttgcct gaggcagtgt ttgccactga cagcctgaaa ttaatgagca
    11461 tttatcgtgc ggcggcagag aaaaacattg ctattccgca gcagttagcg gtggtgggtt
    11521 atagcaatga aacgctgtca tttattttaa cgcctgcacc gggcggcatc gatgttccga
    11581 cgcaggagtt aggtcgacaa agctgtgagt tattattccg cttaattgcc ggaaaaccgt
    11641 caccacaaaa tattaccgtt gccacgcata tgacgctgaa ataaatacca gcgaccttgc
    11701 ctgaatgccg gatgcgatgc tggcgcatct tatccggcct acatttatag atcggataaa
    11761 gtactcgcgc cgcatccggc aatttagccc attatcagaa cgagtaattt acgccgacac
    11821 ctgcataatg gaatttatcg ctgtcgcctt cgtcgtgatc ctgccactca aacgcgtatt
    11881 ccagcgaaat ggaaagacca ttctggaaat cataaccgta aaataaacca acacggttaa
    11941 aatcatggcc ttcacgttca atatcatcct gccagtccca gttactccag cgatccagcc
    12001 caatgcgcgt atacggcgtc accgagtggt tgccgagcgt cagcggcaaa taggcgcgaa
    12061 tttcttgcgt ggaaaactca ccgttattac ggctgtcgtc catattgaag ccgcgctcga
    12121 gataatagtt cactcgcaag gcaaccgttt cgttgaaggt atattgcaga cctgtttccg
    12181 tttcgacacg ggtatcagcg taaccggtcg tgttcaaatc gttggcaaat ttatacatcg
    12241 acaaccaacc gttgaaacgt aaatcgtcag tcagtttcac atcccagtct ggcgcgattt
    12301 tccagcgctg catattcgcc gtgtctttac ccggttcatc aacgtagtga taaccataat
    12361 tacggaaacc gccggtcagg ccgaaactga aatcatcgct ttcgaggaac tgataatgta
    12421 cctccagctc cgggcgatca aaccacgttc cacgtttacc cgcgctataa tctaccggcc
    12481 cttcctgata ataggccaga gaaattctcc acggcccgtt ggcggcatta aaatagacag
    12541 aaggctccgc cagcccatcc atatcttcgc cataaccctc gacgttttct atttcgtaca
    12601 tcgcgccgat attaaagtgc cagtcgttct tttcctcggc ctgtgcgcag gccattcccg
    12661 cacacatcac cagtgcggta cagggtaata actttttcat tattgttcct tgtattatcc
    12721 tgggggaatg ccttccccct gtatttagcg aattgctatt tccgtttctg catcaaagaa
    12781 atgacattta gtcatatcaa aatgaatagt gatattttct cctgcatgat aatcatttaa
    12841 cgcaccagca cggaccacta actcgtgccc cccaaccgtg gtgtagagca taaattccgc
    12901 accggttaat tctaccacgc taattttggc ggaaatatta ttttcctctt gcgcgtccgg
    12961 atgaatatct tccgggcgca ttcccatcac gatgggctta tgcaaacttt cctgtgtttt
    13021 cagaaccgct aatttctctt cgggaatggt taatttaagc gtttccgtaa cgaatttatc
    13081 gccatcgatc gtgccgcgaa taaaattcat cgctggtgat ccaataaatc cggcaacaaa
    13141 catattcgcg ggttggttat aaacggtttt cggcgcaccc acttgctgaa caatcccgtc
    13201 tttcataatc acaatccgcg tcgccatggt catcgcttcg gtctgatcgt gggtcacgta
    13261 gatcatggtg gtgttcagtt tctgatgcag cttgctgatc tctgcgcgca tttgtacgcg
    13321 cagcttggca tcaaggttag agagcggttc atccattaaa aacacgcccg cttcgcgcac
    13381 aatcgcccgc ccgagcgcca cacgctggcg ttgcccgccg gaaagcgcgc ccggcttgcg
    13441 cttcaggtac tcacgcaggc cgagaatttg cgccgcccag ttcacccgct catcaatcac
    13501 ctctttggcg attttttgca tcttcaggcc aaacgccatg ttgtcgtaaa ccgtcatatg
    13561 cggatacaac gcgtagttct ggaacaccat tgcgatattg cgtgctttgg ctggaacgtc
    13621 attcattcgt ttgccgtcga tcaacagatc gccgccgctg atctcctcaa gcccggcaat
    13681 catgcgcagg gtggtcgatt taccgcagcc cgacgggccg acaaatacga tgaactcttt
    13741 atcggcaatt tccaggttga agtccttcac cacatgcacc tggttatcgt agattttttg
    13801 aatatgttgt aacgaaagct gagccatgtt gattcctttg ctatacgttt tgccagaagg
    13861 ccgataaccg cggccaggtc agggagtccg ttgagggcaa caataattgc gccccggtta
    13921 agcctgcgcc gatccccacc gagcgcatac cgctggcgtt aatggcgtca atgcccgcct
    13981 gcgcatcttc aatgccgata catgcctgcg gcggcacgcc cagcccggca caggcagcga
    14041 gaaagatttc cggatccggt ttcgagtttt taagttggga agcatccgcg cagaaggtga
    14101 aaaactcgcg cagctccagc gccgctaaaa tcgtcggcgc attcagggag acagaagcca
    14161 gccctaccgg gatctgctgt gcgcggagct ctgccagcaa attgcgaata ccgggcagca
    14221 cagcattaac cgtcagctcg cgcagtgagt ggacgtaaag cagatttttg cgatacgcca
    14281 gttgcgtccg ctcctgcggg ttaaagtcgc cctctttgcc cccgtgttgc agaatgcgcc
    14341 gcagagactc atcgcggctg atccctttta gggattcgtt aaactgcgca tcaatgctga
    14401 tgccaatttc agcggcaatc tgctgccacg cctggaaatg cagatgcgcg gtatcggtga
    14461 ttacaccatc cagatcgaaa attacccctt gcagtttcat tcatcctcct gatgcgtggt
    14521 agcggtccca ttgaagggca aaataaaatc acccaaacag aaaacagatt cttcagccac
    14581 ggatataagc tgcccgttca aacgcagtga aacgggcgca gaagttcgaa tcgcaatacg
    14641 ctgcgcgtcg agcgtgacct gtaattcgca gccctgccag aacagaggga aagacaactg
    14701 ttgccactgc tccggtaacg ccggattgag atgtaattca ccgtcacgca cgctcacccc
    14761 ggcaaaaccc tgaatcgccc ccagccagat agcgccagtt gcggcagcgt ggataccatc
    14821 atcacaacta tgcggatcag caccaagatc gatttcagtc ccctcgcgcc agaactgata
    14881 actttgggtc agcaggccac agcgtgcggc aacaatgccg tggattgctt tacttaatga
    14941 cgagtcgtga atagtgcgcg gttcataaaa ttgcagattg gcaagacacg ataccgctga
    15001 gaactgctcc ggcagcatgt aattgagcat caccacatca gcttgtttga ggatctgcat
    15061 ctcgttcact tctgcgcgtg aataatccag cagaatggtt tgcttccccg ccgccgcttt
    15121 gtatttagcc agattaatcg ccggcttagc cataaacgaa tcatcctgcg gcaaaacgcc
    15181 atcgggctgc gtttctggca tccatagctc tttgaggaac atttcggcgc gatggataaa
    15241 cgcatcgtcg ctacagccga actggcgggc aatattcagc gcctgttgaa cgttgtagcg
    15301 ggccatatag ctggtgaagg cgttattatt gacatgttcg gtatattcgt ctggcccaat
    15361 aacatcatga atttccagac ggtcgttaac cctcaccgcg cggctaatcc agaactttgc
    15421 agtttccaga agtagcgcca tgccttcatg agcaatgaaa ctttcatccc ccgtggtctg
    15481 ccagtattga ataaccgccc aggcgatatc ggccaccaga tgatgttccg cctgcgccga
    15541 ggccactttt tgccgcagcc cggtacgaat gttaatggcg gcaaattctg gcgtctcttc
    15601 ttcgccgctg cgcgcgcttt cccacggaaa tagcgcgccc tgccagccgc tgcgccgtgc
    15661 tttctcctgc gcgcctggca agttgtgcca gcgataacgc agtaaacttc gggcaaccgt
    15721 cggatcgcta aacagatgga acggcagcaa aaatacttct gtatcccaga aaacgtggcc
    15781 tttgtagcct tcgccggtta agccttttgc cgcaatactg ctgcgctcgt cgtgagccgg
    15841 cgtcatgatg cgcagatgat aaagcgcata atctaacgct tgctgatcgt gcgcatcgcc
    15901 gccatttacc gtgatacgac gtttctgcca ccattgacgc cagttttctg ttgatgctgc
    15961 aagaagttgg tcgtaactct gctgcgcgca tatttcaagc tggcgaagcg acgcgctgcc
    16021 ccactcgtct aaaacggctt gcctgtcatc ccgccagtcg atccacacca gtttttgcaa
    16081 cgtcactgtc tcgcctgcat gaagctgcgc actggtatgt tgcagcaaac ggcgctcttt
    16141 ggcggtatag cattgctgca catcaccgct caccttacag caacagctga tggccacatc
    16201 actgcgtcca tcctgggtgg tgtagatccc ctgcatcaga tgctgaccaa acacccgcac
    16261 ctgggtttcg tcgagatgtt ggcgaccgtg gttggtttgc gtggcgtcga tacctgttga
    16321 aatcagcact gacgcgtcgg cgtccagtgg cgtaatagtg atttccagcg caatgagcgg
    16381 cagttggtct gccgaaacaa agcgacggct ggcgatggtg taacctgcgc cgttgctggt
    16441 acgccagaca acgttgcggc gtaattcgcc actggcaaag tcaagctcac gctgccaggc
    16501 ttcgtgggat aacgagaaaa cctcaccatt tatggcaatc tccatcccca agatatcagg
    16561 caggttcacc agttcgttga tttcaccttt tcccgcccga tgatacagcc ccgccagata
    16621 catccctcgc gtctggcgcg tgtaatcttc ttcatggctg gcgcgaagcc caagatagcc
    16681 gttcccctgc gccatcagcg atgcatactt gttcagggta tgctggctga aatggggttc
    16741 tgataacgtt actggcctgg tcataattcc acacgcgtcc ctgtttctgc ggattgatat
    16801 aacgccgcca ccagttgctg gatgatgtac ccctgctcgg cgtcggctat catcacgggc
    16861 ttgccctgta cgtgattgat aaaggcttcc atgctgccca aatggcggtt gtcgtctgcc
    16921 atttcccgtt gcatcagcgt cattaattca ccgttgttat cggtgtagat atgtgctgga
    16981 aacagcgtcg caccggcttt atcaccacaa aagctgacgt tcataatcga ctgttcgcgg
    17041 atgttgagcg caaatgacgt ttccagccac agaatgccgc cgttatgaaa ttcaatggtg
    17101 ccaaacagcg aatcttcgac gctgtaagtt gccggatccc actcgccaaa ttgaccacag
    17161 ctcttttgcg tgccgatctt ttgaaagcta tgcgcattca cgcttttcac cgcaggatag
    17221 cccagcacat acatcgcagc atccagcata tgaatgccga tgtcgatcag cgggccacca
    17281 ccctgcagtt ctttattggt aaagacaccc cagccgggaa cgccgcagcg acgcagggcg
    17341 cgggcggtgg taacgtaaat ctctcccaaa acgccgttgg tcacctgttc acgcagctgt
    17401 tgcgtatcga gcgcaaaacg atggtgaaag tcgtaggcca gcaccttgcc ctgtttgcgc
    17461 gcggtatcgc acatttcccg cgcctgttct ggcgtcatgg cgggcggttt ttcgcacatc
    17521 acatggcagc ccgcttccag cgccatcagg gtatgttcgt aatgaaaacg gttaggtgag
    17581 cagacgctaa ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg
    17641 gatgcattcc cgtatttttc tgccagcgcc tgcgcctggg aaaggcggct gtcacagaca
    17701 gccaccagtt ccagatcgtt gcgggtgcag tagtacgaag catgaacctt atccgccacc
    17761 tggcctgcgc ctattatcgc gacccgcagt ggagagcttg tcattgcact tttcacaaag
    17821 acctcttagc aggtacgcaa ccaggtcaac gaatcacggt acgcctgggc aggatcttct
    17881 gcccggatac gcccttcata caccacataa ccctgatagt tatcggcgcg cagctgttca
    17941 aacagcgcgt ggaaatcgag ggtgccgctg cccggctggt aacgatggtt atcggcaata
    18001 tgcacatgac cgagcaagtc gcggttgtca tgcagcgcct gcgccaggtt atcttcttcg
    18061 atgttcatgt gatagaaatc gccgataatc tgcacatgtt taagattgtt ttcgacgctg
    18121 taacgacgag catcggcgag ggtgttgatc atatgatcct gatagcggtt taacggttcg
    18181 agatacacca cggttccggt acgcgcggcg acctgttcca gtacgcgcag ggaatcactc
    18241 accattttgc ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa gcggaaggta
    18301 aacatgcccc acgctgccgg aacgacgata cctttacccc ccacttctgc cagcgcttcg
    18361 agaatgcgtt cgatctgctt taagccatta agacgacgct cttcgataaa gtcaccaatc
    18421 cagccgtcat agccgccgca ggcggtggtc accggtaaac cggtttcttt gatcgccgat
    18481 ttgacttctt cgatgttgtt gaccagcagt ttgccgtcaa tctcaaaacc atcgaagccc
    18541 atctctttga tataacgaaa tttctccaga atattttccg ggaagaacgc ctgattctgt
    18601 gtgcctattt tcattttttc attcctttga attaaaacgt gacgcccatt ttgatgctct
    18661 gttccggatg ctgatcgaca tactgcatgt agctttccgg gctgttggca aaggtcacta
    18721 ccgggtcgat taaatcttcg caattgagat aaccgttcat cagcagtttc cagcaggttt
    18781 cttcaatacg cttacggctc cagcgcggat aatccgggtt cggttcactg cacgcgcgag
    18841 agaagacaat tttggcgtta ttgaaatgcg cttcgcgtcc gaggttaaaa ccttcagcaa
    18901 acggtttagc aaacgcgaca taggagatgg tgccaccgta agccaggccg cgcagcgccg
    18961 actgcagcgc gtcggcgtaa ccgctggttt cgataatcac atcggcaccc tgcttgccgg
    19021 tcagcgtttt gatctctttg cctacgtcag tgccaatggg attaaggcag aaatccgcac
    19081 cgtgacgacg agcaatatca caacgatggg caataggatc gacgccaatt accaccgacg
    19141 cgccagcgcg tttagccagt tggatggcaa tttgaccgat cgcgccaagc cctaccacca
    19201 ctacaaaatc ccctacgcgt acgttggcat cgcgaacgcc actcatggca aactgcgccg
    19261 ggtcgtagca gacagcgttt ttccaggagc tgccttccgg cattttgcgc agcttgtagt
    19321 tattcactgc gttaatgatg atcgtctcgg agagcgggcc gtagccgcat acgctgtcgc
    19381 ccaccgcgta gtcggtaacg tcgctgccgc actcgataat gtcgccaacc accatgttgc
    19441 caagctggaa tttgccaaac tcaatgccgc gcggcgcatc tgccggacgc ggggtgaaca
    19501 tctgccattc gccgttaaag tcttcatcga taaacgggct ggcggcgcgg aagtcgacca
    19561 cttccgttcc gtgtttcggt gcaccgaaac gcacgcggat cttcacttca ttggctaaaa
    19621 tcgcccgatc ttcatactca accagcgcag caacgcgcgg tgctgtggct actaactttt
    19681 tcatgtaaaa ctccttgtaa agaaactggc cgctttagcc cttcacgcca ccggcggtca
    19741 aaccactctt aataaaacgt tccgaaagcg catacataat caccaccggc aatgcggtca
    19801 ccagcgaggc cgccatcatc cgtccccaga tgtaatctgg cgtactgaac agcgcgttca
    19861 ggccgactgg taaggtgaaa ttgctggcgc tggagaggaa aatcgacgca aacagatagt
    19921 cgttccatgc caccataaag cagtagacga atacggatat cagcccggac atcgccaggg
    19981 gcacggtgat acggaagata atttgcagac ggttgaggcc atccatcatc gccgcttctt
    20041 cgatctcatc agggatggtg tcgaagtaac ttttcagcat aaacacggcg gtgggcagag
    20101 tttgcgtcac catggtaatg atcagcgcca tctcggtgtc gtaaatgcca agtgcggtga
    20161 taattttgaa cagcggcacc accagcaaaa tgccggagaa catatacacc gtgtaaaagc
    20221 tggcgttgat ggtcatccgg cctttaaagc gcaggcgaga aagcgcgtaa gctccgagaa
    20281 tgccgagaaa taccgccacc acggatgaaa ccaccgacac caccatgctg ttacggaagt
    20341 agtcgacaaa cgggaaaatc accgggttaa aaatgtcgac ataatgctcc agcgtccact
    20401 gttgcggcag cagcgtagga tgcagcgaga tcgcctcttt tgcgctcttg aacgaggtca
    20461 tcagcatcac aaaaaacgga aacagcgtga tgatgagaaa cagcgccagc ccgcggtaaa
    20521 aaccgatacg actgagtgtg cgtttatttg ttgccattga ggttcacccg tttcctggtc
    20581 agcagaatga cagcgaaaat aatgatgaag agcaccaccg agatcgccgc cgctttccct
    20641 aagtcattaa aggcaaaggc ggttttgtag agatagacac caagaatatc gaccttggtt
    20701 gtcagcagat aaacatccgc gaacatgtag aacatccaga tggtgcgaag ggtcaccacc
    20761 gtcgccagga ccggcataat cgcgggcagc gtgacgatac gaaaccgttg ccaggcatta
    20821 gcgccatcca tctctgccgc ttcgtacagc gatttgtcga tggtctgcaa aatcgccaga
    20881 aacgagataa aggcatacgg gaagtagcgc cagatggcga agatcaccac cagcacaaaa
    20941 ctactgcccg gattatcgaa ccacagcggt gcctgctcat aaagatgcag aagatcgacg
    21001 ccgaggtagt tcacaatgcc gtagccgttg ttgaacatgt atttccaggc gaacaccagc
    21061 gaaatagacg gcgttacgta ggagaggatc accagcgaac gcgcggtttt gcgcaggcgg
    21121 aactcgcggt tgaaaaacat cgccactgcc agcccgagca cggtgctgcc cgccaccacc
    21181 aacgcggtat accagaccgt catccacagc gagtgccaga agccgggatc ggagagaata
    21241 cgcacatagt tgcttacccc aacaaacgtt gactcgatgt tgggattgag cggcagacgt
    21301 aaaaaactaa tttcgatatt cgacaccatt ggccacgcaa ccagaccgcc cagcagtaat
    21361 aagctgggtg cgagaagcaa cagcgcaaag ggcatatcgg aacgacctga aaaaagcctg
    21421 ttcattgcgg attcctgtcc tgggtttagt gctgttcgat cagttcatcc agctttttct
    21481 ggctcgtttg cagcgtgcca gggagatcgg ctttacctac ggtgacgtta tgcaccatgg
    21541 agctcaccac cccagacccc gtcacatcgc ccatgcgggt aaatttttta tcccctactg
    21601 cgccaaagac ctgaatattc ggcagttcac cgattaactg atttggcagt tcacccagcg
    21661 ccttaataac gtcgttgtct ttccaggtgg aagtagtgac aaccgctttg ttcaccggca
    21721 gcgccgcgcc tggcgacatc atcacccagt cggcaatgtt gtctgcctgc tccataaagg
    21781 tgacaaattt ttcagccgct tccgtttctt cagtcttttg tccggcggta atggtcagcg
    21841 aggtcaacat gccgtagacg gcagagtttt tctcggttgg caccacgaaa ccgacgtttt
    21901 tcggatcgcc ttctttaatc acagccggaa ggatataggt ggagtaaatc gccatcggcg
    21961 cggtgccgtt cataaaggcg tctttcactt ccatgatgtc gttagaaccc ggcatggtgt
    22021 tggcagcaag gtcgcggtaa taggtcagcg cctgcatcat ctctggtgaa tcaagggtga
    22081 ttttgccttc ggcgttaaag acgttggcct agttggataa cgcaaactgg gagaaggatt
    22141 gttccgtcag cacgctttct gccgtaggca gcgcaatacc gtatttttta ttcgccgggt
    22201 cattcagttt ctgtgcaacg tccagcagct gttgccagtt tttcggctcc tccagccccg
    22261 cttttgccag cacatctttg cgataccaga tgccgccaat ccaggcgctg acaggaacac
    22321 cggtccatgc gctaccatct tcggtacgca caatacgcag tacgccatcg taaaacgcgc
    22381 cttcaccaac gttgctgatg accgtggcaa ccgccttgcg atcgataagc tgctctttgt
    22441 ccataacttt ggcgtagtca tgactggttt caatcacttc cggcagcgag ccgctacgtg
    22501 aaagagtgat gactttggtg ttataagcgt cctcttcgac gggcacttgt ttaacggtga
    22561 tacctgggtt ttctttttca aaacgggcaa tcaatttgct gataactgcc tgacgctcct
    22621 gctccaccga agaatgcata aattcgatgg atacatgggg tttattttct tctttacagc
    22681 ctgaaatcag ggcgcaggaa accagtgctg ataacagcac gatttttgat ttaatcatga
    22741 gatgttcctt tttatttaat ccacataacc tgccaggggc gcagcgttat tttattaccc
    22801 aatatatcaa cttcactaat cagatcccga ccgtgcaggc cagtaatatt tacatgctga
    22861 atatttttac tgacattaaa caatccggtc agacaattac cctctgcgtt acttctttga
    22921 atacgcatta cggatgaatt aaccgtatcg atggtaaaat tattatccgg atgaaactcg
    22981 ttatggctgc gacgaagtgt aattaaacgc gacaactcat gatataccgc atgccttaat
    23041 gtagcttcat cgttcagttc tcgggttatc tcttcactgt gatatttttt acggttaatc
    23101 gcacggttat atccgagttt ttcgacacct gcgtaatcat tacgcgagcc cagaatactt
    23161 tgaatatata tcgctggcac accggggaaa cttaacaaaa tcgcatgggc aaggataaac
    23221 ctggcgcaac gttcttcatc gctactctcg cggcggctta acgcatccat ataggtgaca
    23281 tttatttcat agggactgcg cgtaccgtcg ggattatttt tccagtttac taatgcacct
    23341 tcctgctgta atgcctcgac cagcgctaat atttcgcttt ctggtaacaa gccccgcagt
    23401 gggttcagcc cgatgccatc gtgagaggcg aggaagttaa accaggtcgt tttaccggag
    23461 ggcaagctca gactttgcgc ccactggcac agcgcctcga cgttctgttt ttgcaccgca
    23521 tgtaacacca gcggcggcag cgagaactgg tacaccatat gcgcttcgtc atcgccttcg
    23581 ccaaagtaag caatgttatc tttatgcgga acattcgtct cagtaataat caccgtacct
    23641 ggcgcgatgt catcaataat cgaccgtaac agtttgataa tcagatgtgt tttttccaga
    23701 tggatgcagc ttgttcccgg ctctttccac ataaagccaa cggcatccag acggacatat
    23761 tctgcgccct tttccaggta acaaagcaaa acatccacca tcgccagcaa cacgtcaggg
    23821 ctacggtaat tcaggtcaat ttgatcgtcg ctaaaggtgg tccataaatg gcgcgtggaa
    23881 ttatcatcca tttgaaacgg cgttaatagc ggtaatgcac gcggacgggt gacagcgctg
    23941 agatcggttt gcggatcaac cgcaataaaa aaatcttcaa aacctggctg ctgttgtaaa
    24001 tagtttttaa accattcact ttttgccgac atatggttgc agacaaaatc aaacattaaa
    24061 tgactgcatt caccgagctg ctgaatatcc tgccactacc cgcttcactg gcgacctgat
    24121 gataatcaat taccgaaaat ccatcatcag atgaccatgg ataaaaaggt aataaatgaa
    24181 catgtgaaaa aatgctttgc agccattgat ggtaaaactg attaaatgtg ggtagtggtt
    24241 ttaaatcatt actgtgaaat tgatcggcat aggtaattaa cacgacatca ctttcatccc
    24301 agtgtttttt acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc gttacaagtt
    24361 tctgtaaatg agttgcggta aatgttccac cgtagatttc gtccaggtaa tccgtaattt
    24421 tctgtttcat gttgcctttc ctggtagcgg tcccatgaaa tgagagacta ctccgcagaa
    24481 aataaactgt caattgagca caagggtttt agtcacgcag gaaatcattt tttaccaggg
    24541 aaaaagcgta tttgtgagct gacgcaaata atctataggg atgtaaataa ggtgtgacag
    24601 aaaaaaacgg cgcataagcg ccgctcatgg tgaattcttt taacctttga ccttcggcat
    24661 tgcgatgtct ttcaggccac cggcgttctc aacgtgggta tatcccatct cgctaaggat
    24721 ctcttttgcc tgccctgatt ggcgtccggc attgcaatac actttcacgg tgtcgttttt
    24781 atccggaacg acggtggcaa tgcgctcttt cacttccttc aggggaatat tgatggcccc
    24841 ctgaacgtgc tcttgctgat actgctctgg aacacgaaca tcgatccagt gttcagcggc
    24901 gaaaacgggc agtgaaaaca ccagtgccag agcaagtaag ccttttttaa acatgtaagc
    24961 gtccttctat ggatgaatat tgctatccat tatacgcctg agatttaaca atttttgcga
    25021 aactcccctc accttttata acgctgcgcc agtttattgg cagcccgact taacagcggt
    25081 tccagcgcca cagccagcaa cattttcagc ggtcggcgag caactgattt tatcgcccag
    25141 cccgccacac ccgctgggcc atagcgcagt gcggtaagaa gtaccagctt gcctgctaat
    25201 ttgaaaccag gctttacctt ttgcccggcc tgttgccagc gagtattcat aactttcctc
    25261 acagttgacg gaaacggcta cgtaacgtga atgtatcgga agtgacataa cgttccatct
    25321 cgcgtaaacg cgtttcactt gccgccagtt cacggtcgac ttcatccagc aattcactgc
    25381 tggaaggtag ctgctcacca aaggccatgt tgtccggcat tggatcaagc gcaaatgaca
    25441 aaatgatgta agcaaccagg gtaaacagcg ccagaccgaa gaaaatcgac aacaccacca
    25501 ggatacgcac cagttttacc ggtacatcaa aatagttggc aatcccggcg cagacgccac
    25561 ggaccatgcc ctgctgtgga atacgccata attttttatt gagattaatg cccgccatta
    25621 gcgatccctc cagttcggat gttctgcatc aagaatagat tccagcgcct gaatacgttc
    25681 gcgcatccgt tttgcttcat cagccagttg cgctaatcgc tgctgctcac tttgcgacaa
    25741 ttcactgcga ccagaacgat tgctgtaatg cagccataac cagatcggta aaacaaacag
    25801 cacaaaaatg gttaatggaa tagccagaaa tagcgcgctc ataagtactc cttagacgat
    25861 gagcggcggc gcgttcggac gccgcctgga tgttattatt gattgtcttg cttcattttg
    25921 gcttttaatt gtgccagttg ctcgctgatt gcatcatcgg ctttcagttc ggcaaactga
    25981 tcgtccagcg atttttgttt accgaagctg tggctttctg cttccgcttc catctggtca
    26041 atacgacgtt cgaaagattc gaaacgagcc attgcttcat ccagtttgcc actgtccagc
    26101 tgacgacgca catcgcgcga cgagtttgcc gcctgatggc gtaacatcaa tgcctgctgg
    26161 cgagcgcgtg tttcgctcaa tttgttttcc agctcgccaa tctctttctt catgcgtgcc
    26221 agcgtatcgt ccaccagcgt cacttcatgt tccagggact taatcagatc ggtcagtttc
    26281 tgtttttcaa ttaacgccgc acgcgccaaa tcctctctct ctttcagcag cgccagttcg
    26341 gctttttcct gccattcaac ttcacgcgcc gacgcttgtt caatacggcg agtcagctgt
    26401 ttcttttctg ccagcgcacg cgccgaagta gaacgtactt caaccaatgt atcttccatc
    26461 tcctggatca tcagacgcac cagtttctgt ggatcttccg ctttctctaa cagagcgttg
    26521 atgttggcat tcacgatgtc ggcaaagcga gaaaaaatac ccataatgtt gtcctcttga
    26581 tttctgcatt caggattgtc ctgctgaact gttaatacaa tttgcgtgcc aattttttat
    26641 ctttttgatt tataaagatc tgattgaaga atcaacagca acatgccagg atgaattagc
    26701 taattacact aacaagtggc gaatttcatc atggcagaat acaaagataa tttactcggt
    26761 gaggcgaaca gctttctcga agtgctggaa caggtttcgc atctcgcacc gctggacaaa
    26821 ccggtgctca tcatcggcga acgcggcacc ggtaaagagc tgatcgccag ccgcctgcat
    26881 tatctctcct cccgttggca agggccgttt atttccctta actgcgcggc gttaaatgaa
    26941 aatctgctgg attccgaact gtttggtcac gaagcggggg cgtttaccgg tgcgcaaaaa
    27001 cgtcatccag ggagatttga acgtgccgat ggcggtacgc tatttcttga tgaactcgct
    27061 acggcaccga tgatggtgca ggagaaatta ttgcgcgtga ttgagtacgg tgaactggag
    27121 cgcgttggcg gcagccagcc gttgcaggtg aatgtgcggt tggtatgcgc gacgaatgcc
    27181 gatctcccgg cgatggtcaa tgaaggtact tttcgcgctg acctgctcga ccggctggct
    27241 ttcgatgtag tgcaactgcc gccactacgc gaacgtgaaa gcgacattat gttaatggcg
    27301 gaacactttg ccatccagat gtgtcgggaa atcaagctgc ctctgttccc ggggtttacg
    27361 gagcgcgcca gagaaacatt gctgaattat cgttggccag gaaatattcg tgaattgaaa
    27421 aacgtggtgg aacgttcagt gtatcgccac ggcaccagcg attatccgct tgatgacatc
    27481 attattgatc cctttaaacg gcgtccgcct gaagacgcta tcgccgtttc agaaaccacc
    27541 tcgcttccaa cactgccgct ggatttacgt gagtttcaga tgcagcagga aaaagagttg
    27601 ctgcaactca gtttgcaaca agggaaatat aaccagaagc gcgcagctga attgctgggc
    27661 ttaacctatc atcagttccg cgcgttgttg aaaaagcacc agatttagcg aagttgggca
    27721 atacaacttg ccggatgcgg cgtgaacgcc ttatccggca tacataatca atacgtcgtt
    27781 tgcaggtctg ataagcgcag catatcaggc attattgcgt tgcccctgct tctgcgtatt
    27841 aatctctcag cgcatcctgc aaaattttca ttgccgcatc gaattgcgca tccgggatgg
    27901 tcagcggata caggaaacga atcacgttgc cgtatgcgcc acaggtcagt agaagcagtc
    27961 cctgcgccag cgcgcgttgc tgaatttttt gcgcaatggc ggctgacggc tcgcccgttt
    28021 gcgggtcgtt aaattctgcc gcaatcatcg agcccagacc acgtaccgcc gcgatggccg
    28081 gaacgttttc tttggcatca atcagggttt tcgtcagacg ctgccccaac tgcatggcgc
    28141 gctcacagag tgactctttg tcgataatgt tgagcaccgc gtgcgcggca gccaccgcca
    28201 gcggattccc ggcgtaggtg ccgccaagcc cgcccggcgc gggcgcgtcc ataatattcg
    28261 cgttaccgac cacacccgaa agcggcatcc cgcccgcgag gcttttcgcc atcgtcatta
    28321 agtccggctt atcggcgtaa tgatccatcg caaacagttt gccggtacgc gcaaagccgc
    28381 tttgcacttc atcggcaatc atcacaatac cgtgctcgtc gcacaggcgg cgaatggcgg
    28441 caaccagctc ttttggcgca acgttgaaac cgccctcgcc ctgcaccggt tcgaaaataa
    28501 tggccgcgac ctgcttcgct tcgatgtctg atttaaacaa gcgttcgatg gcgtcgaggg
    28561 agtcctgtgt tgaaatgccg tgtaaatctg acggataagg tacgtgatac accgaaccag
    28621 ggaacgggcc gaagccgatt ttgtacggcg cgacctttcc agttaacgcc atagtcatat
    28681 acgtacgacc gtggaagccg ccgctaaacg caatcacgcc agggcgtccg gtatgggcgc
    28741 gggcgatttt caccgcgttt tccaccgctt ccgcaccggt ggtgaagaac gcggttttgg
    28801 cctgcccgct caccggggca agggcgttga ttttctccgc cagggtgacg tagctttcat
    28861 atggcacaat ctgatacgcg gtgtgggtaa actgttgcag ctgctgctcc accgccgcga
    28921 ccagatcagg gtggcgatgt ccggtattca gcaccgcaat gcctgcggcg aaatcgatgt
    28981 actcgttgcc ctcaacatcc ttcagcgtgg cgttttcagc cgactgggcg aagaagttac
    29041 acatcacgcc aaccccgcgc ggggtggcag aaagacgacg ctgatggaat tcattgttgc
    29101 tcatgtttca caccttttga ctcttcactg ttaatcggaa attaaacgcc cagacgatcg
    29161 cgcaggctgt aatacgccgc gcccatcgcg gtaaacggta cgcgcagcgt gcgcccgccg
    29221 gggaacgggt aatgcggcag attggcgaag gcatcgaaac gttcggcgtc gccgcgcagc
    29281 agttcggcaa tcaaacgtcc ggcgagatga gtacaggtga cgccgtggcc gctgtaaccc
    29341 tgcatgtaat agatgttggt atcgaggcga ccgaactgcg gcatacgcga cagggtcagc
    29401 aggaagttgc ccgtccagcg atagtcgatt ttcacgccct tcaactgcgg gaaggttttc
    29461 agcagtttcg gcaccacaag acgctcaacg tcatccgggt cgcgcgcgcc gtacaccacg
    29521 ccgccgccgt acagcaaacg gttgtcggcg gtaagacggt agtaatcaag cagataatta
    29581 cagtcttcca cgcagtagtt tttcgggatc agcgaacggg ctaaatcatc ggaaagccgc
    29641 tcggtggtga tcacctgcgt accgcacggc atgctgcgtt tcgccagttc cggctccact
    29701 ttatcgccca ggtacgcatt cccggcgaca atcacatact tcgccgtcac ctgacctgtg
    29761 gctgtacgca ctacggcggg cgtggtgtgc tggatctgcg tcactgcgga aagctcatac
    29821 acgcgcccgc cgttcaggcg gatagcgtcc gcttcgccaa tggcgaggtt cagcgggtga
    29881 atatgcccgc cgctgtgatc cagcagcgca ccagtgtagc gatcgctggc gacctcgcgg
    29941 cgaatggcgt tggcgtccag catctccagc tgtttattgc cgtagcgttc ccagttctct
    30001 ttctgctctt caagtgtggc gagctgttta tcattcatcg ccacaaacag accgccgggg
    30061 cggtagtcgc agtcaatctg ataacgtttg atacgttcgc ggatgatctc accaccttcg
    30121 aacatcatgc tgccgagcat acgggcggtg tccatgccgt agcttttttc gataacgtcg
    30181 atgtcgcggc tgtaggagtt cacaagctgc ccgccgttgc gcccgctggc gccaaagccg
    30241 atgcgtgagg cttccagaac caccacgtca aagcccgctt ccgccagatg cagtgcggaa
    30301 gacagcccgg tatagccgcc gccaaccacg caaacgtcgc aggaaattga ctcattcagc
    30361 gtgtcgaatg gcgcgtattt attcgcactg gcggcgtagt aactgctggt atgttctgtc
    30421 atttcaggcc tccaggctta tccagatggt tttcagttcg gtgaattttt caagggcatg
    30481 cagggatttg tcgcgaccgt tgccgctctg cttatagccg ccaaacggca cggtcatatc
    30541 gccgtcgttg tagttattga cgaagacgga accggctttc aggcgtcggc tcatgcggtg
    30601 cgcgcgggag aggtcgcgcg tccataccgc cgcgccaagg ccgtactggc tgtcgttggc
    30661 aagctgtagc gcctgttctt ctgatgtgaa acgcgtgacc accagcaccg gaccgaaaat
    30721 ctcttcgcgg cttaaggacg cattcgggtc tacatccaca atgatggtcg ggccgatggc
    30781 gacagccagc ccggcgttac ggccatccaa caacagttgc cctttgcttt cgccttcctg
    30841 aataaagcta tggaccgagt cggcgtgggc gcagtcgatt aaggtgccca tggtggttgc
    30901 gggatcaagt ggatggcccg gctgccagtt ttgcgcctgc tgttttaaca gggctaagaa
    30961 ttcatcggct atgctctctt ccagcaacag gcgcgttccg gcgatgcaca cctgcccctg
    31021 gttgtagaag atgcctgctg cggtggcgct tgccgcctgt tgcaaatccg ggcagtcagc
    31081 gaaaacgatg ttggcgcttt tgccgcccgc ttccagccag acgcgtttca tgttgctgtc
    31141 gcctgcatct ttcagcagct gtttcccggt acgggtcgaa ccggtaaagg caatggcgtc
    31201 gatatcgtta tgacgcgaca gcgcctgccc cgcttcatga ccaaaacccg tcaccacgtt
    31261 caacacacca tccggcaagc ctgcttcttt cgccagcccc gcgagacgaa tcgcactgag
    31321 cggtgatttt tcagacggtt tcaggacaac gctgttccct gctgccagcg ccgggccaag
    31381 cttccagcaa gtcagcagca gcgggaagtt ccacggcacg atggcggcaa tcacgccgac
    31441 cggttcacgc acgatcatcg ccagctcatg gctactggtg gtcgccactt cgccatacac
    31501 tttgtcgatc gcttcggcgt accagcgaat ggcgcgcgcc gcgccgggta tatcatcacg
    31561 cagactgtga cgaatcggtt tgccggtgtc gagagtttcc agcagtgcca gctcttcggc
    31621 gttggcttct attaaatcgg cgagtttatt cagcaccgct ttacgttttg ccggagaaga
    31681 gagtgaccag tcgccgcgtt caaatacgcc gcgtgctgcg ctcaccgcac ggtcgatatc
    31741 gacgctcttg ccgcgcgcaa tattcgccag cggtgcctgg gtaaccggat caacggtttc
    31801 aaaggtttca ttttccgccg cagcagtata ttcaccgtta ataaataagc ggttttcaat
    31861 ggcgagactt aacgctttat cctgccagta agccagatga tgaaaattca ttatgactcc
    31921 tgtttcacgt ctatcagata tatgcagttt gtgaacgtca gggcgcagag atccccgggc
    31981 cgtatgcccg tttatttctg gcgacattct ttatggattg ccgggcgcat tgcgcttacc
    32041 cggcttacag aacaaaaaga ttaaaacgtg gtgggcgtat gggcgctgat aattcggcaa
    32101 atacctgccg acgtattact gaaactgtgc gggatgccgg tattaatggc ataactttgc
    32161 cccgcgacga ggtggtaatc ctgaccatta atcgtcagaa taatttcacc ttccagtaca
    32221 gtgcctattt cctcaccctg atgcttaatt ctttccccag tggttgtgcc cggctggtac
    32281 gtttcaaaga tcatcgccag cgtgcgattc gggttaccgt tatgaaccag cttcattgac
    32341 acaccctgac tgcccatctc aattaaatcg tcctgattaa tgacgacctg cggctcatca
    32401 ggtttttccg gctcggaaaa gaattccgag agtgacagac catacacctt cagcagcttt
    32461 tgcagcgtac tgatggcagg gctgacttta tcttgttcta tcgtactgat agcactgtga
    32521 gtcagcccgg agagttcggc ggcacgacgt tgtgaaagcc cctgctgctg gcggatttcc
    32581 gacaagcgtt ttcctggcgc cagtccctca tcactcatat ttgcatttcc ttaaactgta
    32641 gtggtcagag tcgctgtttt tcagcgatat ggtgctgaca agcggtgata aagccctcga
    32701 acaatatacg cgaaagcgcg tactcgctac tgttccattc cgggtgccac tgtacgccca
    32761 gcgcaaaagg atgattgatg acactaaccg cctcaactaa gccatccggc gagcgagctt
    32821 caacacgcaa ccgtgggcta acgaccttcg ccccttgtcc atgcagagag tttacccaaa
    32881 agttgctaca ttcaggtaac aatgcagaca gcaatccccc ctcttcaacc tgaacttcat
    32941 gcgacggtgc atattgctgt tccaccggca gttcgggatc ttcccgatgt tccagcaatt
    33001 caggctgttc gcacagcttg cgatgcaacg aaccaccggt tgccaccacc agttcctgta
    33061 aaccccggca gatggcgaaa atggggatgc gcctttcgag tgcggcatta attaacgcca
    33121 tgctcagaag atcacgctcg ggatcggcgt caggctcatc gccgttttca ccatataggt
    33181 gcggctgcac attgctggga ctaccaggaa ggtaaatgcc atcgagtttc ggcaaaagtt
    33241 gttcaagtaa cgacggttcc gccagcgcat gtggcagcgc aattggcaag ccgcctgcgt
    33301 ggatgatggc attcaggtac ttttcttgca gagtctgggt cgcatgcccc ttaagcctgt
    33361 tcctgcacat tacgacaccg ataaccggat tgttcattat attttccatt ctcaatctca
    33421 caaagtggac taaattatcg ccattactgc ggtttaaatg acctttatgt tcaatatttt
    33481 ttcaatctag cagtgggttt tcatttttgc aaactcaatt taacatttga caaacattta
    33541 gtttgcatac agattcgaat ggtggtcatt atattttacg ctttgataac gagcggaaaa
    33601 caaaccaaag gcgaagaatc atggaaacca atatcgttga agtagagaac tttgttcagc
    33661 agtcagaaga gaggcggggt agcgccttta cgcaggaagt gaaacgctac ctggagcgct
    33721 acccgaatac gcaatatgtt gatgttttac ttaccgattt gaatggttgc ttccgtggaa
    33781 aacgtattcc tgtttcaagc ctgaagaagc tcgagaaagg gtgttatttc ccggcctcgg
    33841 tatttgcaat ggatattctg ggcaacgtag tagaagaagc aggtctgggt caggaaatgg
    33901 gcgagccgga tcgtacctgt gttcctgttc tcggttcctt aaccccttct gccgccgatc
    33961 cagagtttat cggtcagatg ctcctgacca tggtcgatga agatggcgct ccctttgacg
    34021 ttgagccgcg gaacgttctt aaccgcctgt ggcagcagct gcgccagcgc ggattgttcc
    34081 cggtcgtagc ggtagagctg gagttctatt tactggatcg ccagcgcgac gctgaagggt
    34141 atctgcaacc gccctgcgcg cccggcactg atgaccgcaa tacacaaagc caggtttact
    34201 ccgttgataa cctcaaccac ttcgccgacg tgctcaatga tattgatgaa ctggcgcagt
    34261 tacagctgat tccggcagat ggcgcggtcg ctgaggcctc gccgggtcag tttgaaatca
    34321 acctttacca tactgataac gtgctggaag cctgcgatga tgcgctggca ctaaaacgcc
    34381 tggtgcgtct gatggcagaa aagcataaga tgcacgccac ttttatggcg aagccgtatg
    34441 aagagcacgc gggcagcgga atgcatatcc atatcagtat gcaaaataat cgtggcgaga
    34501 acgtgctttc tgacacagaa ggcgaagatt cgccgctgct gaaaaagatg ctcgccggga
    34561 tgattgacct gatgccgtca tcaatggcgt tgctggcacc aaacgtgaac tcgtatcgcc
    34621 gcttccagcc ggggatgtat gtaccgacgc aggcgtcgtg gggccataat aaccgtaccg
    34681 tcgccctgcg tattccatgc ggcgatcgtc ataatcaccg cgtggaatat cgcgtggcgg
    34741 gtgccgatgc caacccatat ctggtgatgg cagcgatttt tgccggtatt ttgcatggcc
    34801 ttgataacga gctgccgttg caggaagaag tcgaaggtaa cgggctggaa caggaaggct
    34861 tacccttccc gattcgccag agcgatgccc tgggtgagtt tatcgagaat gatcacctgc
    34921 gccgctattt aggcgaacgg ttttgccatg tgtatcacgc ctgtaaaaat gatgagctgt
    34981 tgcagtttga gcgcctcatc acagaaacag aaattgagtg gatgttgaaa aacgcatagt
    35041 accccttgcg gggttgtttc atcggcctga gcggagccag gcctgacatt tgctggtaat
    35101 ccagacattt cccggatgtc gcgttatgga gcgcaggcag caacgggctt ccgttaacga
    35161 ttaagactgt gacgaggaaa tgagatgatg ccgatgatta aatccccgca tggcgcggga
    35221 ggctgcgtat gcgcccctcc tgcgactgac tggactccgc cccctttgct tcccttgcta
    35281 aacaggtttg atttcaggtc gacacgaccg caaaccttat tacgcagggg aggcagcaat
    35341 tatggctatt aattcaccac tgaatattgc tgcgcaaccc ggcaaaaccc gtctgcgaaa
    35401 atcactgaaa ttgtggcagg ttgtgatgat gggtctggcc tatctcacgc cgatgactgt
    35461 gttcgatacc tttggcattg tatctggcat tagcgacggt cacgtccctg cgtcctattt
    35521 gctggcgctg gcgggcgtgc tgtttaccgc tatcagctac ggcaaactgg ttcgccagtt
    35581 tccggaggcc ggttcggcct atacctacgc gcaaaagtcg attaacccgc acgtcggatt
    35641 tatggtcggc tggtcatcac tgctggatta tctctttttg ccgatgatca acgttttgtt
    35701 ggcgaaaatc tatctctccg ccctcttccc ggaagtgccg ccgtgggtgt gggtggtaac
    35761 cttcgtcgcc attttaaccg ccgcgaatct gaagagcgtc aacctggtcg ctaacttcaa
    35821 taccctgttt gtactggtgc aaatctccat catggtggtg tttatcttcc tggtggttca
    35881 gggactgcat aaaggagaag gcgttggcac cgtctggtca cttcagccgt ttatcagcga
    35941 gaacgcgcac ctgatcccga ttattaccgg ggcgacgatt gtctgtttct cgttcctcgg
    36001 tttcgatgcg gtgaccacac tttcggaaga gacaccagac gccgcacgcg tgatcccgaa
    36061 agccatcttc ctgacggcgg tctatggtgg cgttatcttt atcgcggcgt cgttctttat
    36121 gcagctgttc ttccccgata tcagccgctt taaagacccg gacgccgcac tgcctgaaat
    36181 tgcactctac gtcggcggta agctgttcca gtcgattttc ctctgcacca cgtttgtgaa
    36241 cacgttagcg tctggcctgg cctcacatgc cagcgtgtca cgtctgctgt atgtgatggg
    36301 gcgcgacaat gtgtttccgg agcgcgtgtt tggctatgtg cacccaaaat ggcggactcc
    36361 ggcactgaac gtcattatgg tcggaattgt cgcgatgtcg gcattgttct ttgatttagt
    36421 caccgcgaca gcattgatta acttcggtgc tctggtggcg tttaccttcg tgaatctgtc
    36481 ggtgtttaat catttctggc ggcgtaaagg aatgaataaa agctggaagg atcacttcca
    36541 ctatttgctg atgccgctgg ttggcgcgct gacggtgggt gtgctgtggg ttaacctcga
    36601 gtcaacgtca ctgacactcg gtctggtatg ggcttcgtta ggcggcgcat atttgtggta
    36661 tttgatccgc cgctatcgca aagtgccgct gtacgaaggt gacagaacgc cagtgagcga
    36721 aacgtaatag tcggatgcgc gccgcattat cctgcctgag ggtaatgcgc cgcgccatta
    36781 actataaaaa gatgtatgac ccggatcacg agtgctatca tccgccctta tgcttagcgc
    36841 attaacccca ggggcaaata atgaaccacg atatcccgct aaaatatttt gatattgccg
    36901 atgagtacgc gaccgaatgc gcagagcctg tcgcagacgc tgaacgcaca ccgctggccc
    36961 actacttcca gttactgctc acccgtttga tgaacaatga agagatcagc gaagaagccc
    37021 agcatgaaat ggctgctgaa gcggggatta atcccgtacg cattgatgag atcgcagagt
    37081 ttttgaatca atggggtaat gagtaattat ttaattgcag acgatcggtc agtatcgttg
    37141 tccggataaa gcgttcacgc cgcatccggc taacaatgac acgcactgca gcgtgcttta
    37201 atgggtttct cacgatcgta ccgctctgct cctcgctaag ttgttctgtg agttatagtt
    37261 atagagaaat cacgggtacg aatgttgaac agatggcgac gccatctgtt caacgaccaa
    37321 ccacacggcc ctcattacga cccctgaaca caaacggtaa tggaaaatgt cattaaatat
    37381 tagtaagata gcctctttac ataaaatccc ttaatattat gcgccaggta ttatcgtctc
    37441 ttttggtgat tgctggactt gtgagtggtc aggcaatcgc cgcgcctgaa tcccccccgc
    37501 atgctgatat ccgcgacagc ggttttgtct attgcgtcag cgggcaagtc aacaccttta
    37561 acccatccaa agcgagcagt gggttaattg tcgataccct tgccgcccag ttttatgatc
    37621 gactgctgga tgtcgatccc tatacctatc gcctgatgcc agaacttgcc gaaagctggg
    37681 aagtactcga caacggcgcg acctatcgct tccacctgcg tcgcgatgtc ccgtttcaaa
    37741 aaaccgcctg gtttactccc actcgcaaaa tgaatgccga cgatgtggtg tttaccttcc
    37801 agcgaatttt tgaccgcaac aacccgtggc ataacgtcaa cggcagcaac ttcccctact
    37861 tcgacagcct gcaatttgcc gataacgtga aaagcgtccg taaactggat aatcataccg
    37921 ttgagttccg tctggctcag ccggatgctt cttttttgtg gcacctcgca acccattatg
    37981 cttcggtcat gtcggcagaa tatgcccgga agttagagaa agaagatcgc caggagcaac
    38041 tcgaccgtca accggtcggc actggaccgt atcagttgtc ggaataccgc gccgggcaat
    38101 ttattcgcct acaacgtcat gatgacttct ggcgcggtaa accgttaatg ccgcaggtgg
    38161 tggtggattt aggctccggc ggcaccggac gtctgtcgaa actcctgacc ggggaatgcg
    38221 acgttctggc ctggcctgct gccagccagc tatccatttt gcgtgacgac ccgcgcttgc
    38281 gtttaacgct gcgtcctggg atgaacgtcg cctatctggc atttaacacc gccaaaccgc
    38341 cgctaaataa tcccgctgtc cgccatgcgc tggcactggc gattaataac cagcgcctga
    38401 tgcaatccat ctattatggt acggctgaaa cggcggcctc tattttaccg cgcgcctcgt
    38461 gggcctatga caacgaggct aaaattactg aatacaatcc ggcgaaatcg cgcgaacagt
    38521 tgaagttgtt ggggctggaa aatttaacgc tgaaactgtg ggtgcccaca cgttcgcagg
    38581 cgtggaaccc cagtccactg aaaactgccg aactgattca ggcggatatg gcgcaggttg
    38641 gcgtaaaagt ggtgattgtg ccggtagaag gtcgctttca ggaggcgcgg ttgatggata
    38701 tgagccatga tctgacgtta tccggttggg cgacggacag taacgacccg gacagtttct
    38761 tccgtccgtt actgagctgc gcggcaattc attcacagac caacctcgcc cactggtgcg
    38821 atccgaaatt cgacagcgtg ttgcgtaaga cgctctcctc gcagcagctg gcggcgcgta
    38881 ttgaagccta tgacgaagcg cagagtattc tggcgcagga attgcccatt ttgccgctgg
    38941 cgtcgtcatt gcgtttgcag gcctatcggt acgatatcaa aggtctggta cttagcccgt
    39001 ttggtaacgc ctcctttgct ggggtgtatc gcgagaaaca ggatgaggtg aaaaaaccat
    39061 gattatcttc accttacgcc gcattttgtt attgattgtc accttgttcc tgctgacctt
    39121 cgttggcttc agcttgagct atttcactcc acacgcgccg ttacaaggcg cgtcactgtg
    39181 gaatgcctgg gtgttctggt ttaacggcct gatccactgg gattttggtg tttccagcat
    39241 taacggtcaa ccaattgccg aacagttaaa agaggtcttc ccggcgacga tggaactgtg
    39301 catcctcgcc ttcggttttg cgctgattgt tggcatcccc gtggggatga ttgccgggat
    39361 tacgcgccat aagtggcagg ataacttgat caacgccatc gccttactgg gcttttcaat
    39421 cccggtgttc tggctggcgc ttctgttgac actgttttgt tcactcacgc taggttggtt
    39481 gcccgtttca gggcgtttcg atctgctcta cgaagtgaaa ccgattaccg gttttgcgtt
    39541 gattgatgcc tggctttcgg actcaccatg gcgggatgaa atgatcatga gcgcaatccg
    39601 ccatatgata ttgcccgtga tcaccctgtc ggttgcgcca acaactgaag tgatccgcct
    39661 gatgcgtatc agcactatcg aagtgtatga ccagaactat gtaaaagcgg cggcgacccg
    39721 cggtttgtcg cgctttacta ttttgcgtcg ccacgttctg cataacgcgt taccgccggt
    39781 tattcctcgc ctgggcttac agttttccac catgctgacg ctggcgatga tcaccgaaat
    39841 ggtttttagc tggccgggtt tagggcgctg gttaattaac gccatccgcc agcaggatta
    39901 cgcagccatt tccgccggag tgatggtgtg tggctcactg gtcattattg ttaacgtgat
    39961 ttctgatatt ttgggtgcca tggctaaccc tctgaaacat aaggaatggt atgccttacg
    40021 atagcgtata cagcgaaaag cgcccgccgg gcacgctgcg taccgcctgg cgcaaatttt
    40081 atagtgacgc ctctgcgatg gtcggcctgt acggctgcgc ggggctggct gtactgtgta
    40141 tgtttggcgg ctggtttgcg ccttacggca tcgaccagca atttctcggt taccaattat
    40201 tgccgccatc atggtcgcgc tatggcgaag tttctttctt cctggggact gacgacctcg
    40261 gacgcgatgt tttaagccgt ttactgagcg gagctgcgcc aaccgtgggt ggcgcatttg
    40321 tggtgacact tgccgcgacg atctgtggcc tggtactggg caccttcgcc ggggcgacgc
    40381 acggcctccg ctcagcggtg cttaaccata ttctggatac cttactggcg atcccttccc
    40441 tgctgttggc aattatcgtt gtagcgtttg ccggaccgag tttgtcccac gctatgtttg
    40501 ctgtctggct ggcgctgctg ccgcgtatgg tacgttcgat ttacagcatg gtgcatgacg
    40561 aactggaaaa agagtacgtt atcgccgccc gtctggatgg cgcatcaacg ctgaatattc
    40621 tctggtttgc tgtgatgcct aacatcaccg ccgggctggt caccgagatc acccgcgcac
    40681 tgtcgatggc aattctcgat atcgccgcgc tgggctttct cgatcttggc gcacaactcc
    40741 cctcgcctga atggggagcg atgctcggtg atgcgctgga actgatttat gtcgccccgt
    40801 ggactgtcat gctgccaggt gcggcaatta tgattagcgt gctgttggtt aacctccttg
    40861 gcgacggtgt ccgtcgtgca attattgcgg gggtggaata atgccattac tcgatattcg
    40921 taacctgact attgaattta aaaccggtga tgagtgggtt aaagccgttg accgggtaag
    40981 tatgacctta accgaaggtg aaatccgcgg tcttgttggt gaatccggtt ccggcaaaag
    41041 tttgattgcg aaagcgattt gtggggtgaa taaagataac tggcgtgtta ctgcagaccg
    41101 tatgcgtttt gatgatatcg atttgctgcg tctctccgca cgcgaacggc gcaaactggt
    41161 tggccacaac gtgtcgatga ttttccagga accgcagtcg tgtctagatc cttcagaacg
    41221 tgtgggtcgc cagttgatgc aaaacatccc agcctggacc tataaaggcc gttggtggca
    41281 gcgttttggc tggcgcaaac gccgtgccat tgaactgctg caccgcgtgg gaattaaaga
    41341 tcataaagat gcgatgcgca gttttcccta tgagttgacc gaaggtgaat gtcagaaagt
    41401 gatgatagcc attgcactgg cgaatcaacc gcgtctgctg attgctgacg aaccgacaaa
    41461 ctcaatggag ccaacaaccc aggcgcaaat ctttcgcctg ctgacgcgtc taaaccaaaa
    41521 cagcaatacc actattttgc ttatcagcca tgacttacaa atgcttagcc aatgggcgga
    41581 taaaattaac gtgctttact gcggtcaaac ggtggaaacc gcgccgagta aggagttagt
    41641 gacgatgcca catcatcctt atacccaggc gctgatccgc gcgataccag acttcggaag
    41701 cgcgatgccg cataaaagtc gcctcaatac gctacccggc gctatcccac tgcttgaaca
    41761 gttaccgatt ggttgtcgtc tggggccacg ttgcccgtat gcacaacgag aatgcattgt
    41821 gacgccacgt ttgacggggg cgaaaaatca tctctatgcc tgtcatttcc cgctgaacat
    41881 ggagaaagag tgagatgatc gaaacgctgc ttgaagtgcg taatttaagt aaaacgttcc
    41941 gctaccggac cggttggttt cgtcgtcaga ccgtagaagc ggtaaaaccc ttgagcttta
    42001 cgctacgtga aggccagaca ctggcgatta ttggcgagaa tggttcgggt aaatccacgc
    42061 tggcaaaaat gctggcggga atgatagaac ctaccagcgg cgagttattg attgacgatc
    42121 atccactgca ttttggcgat tattccttcc gcagtcagcg tattcgcatg atttttcagg
    42181 atccttcgac ctcgttgaat ccccgtcaac gtatctcgca aattctggat tttccactgc
    42241 gcctgaacac caacctggaa ccggaacagc gtcgcaaaca gattattgaa acgatgcgta
    42301 tggttgggct gctaccggat cacgtcagtt actatccgca tatgttggcc cccgggcaaa
    42361 aacagcgtct gggtctggcg cgcgcgctga tattgcgccc aaaagtcatt attgccgatg
    42421 aagccctcgc ttcactggat atgtcgatgc gttcgcagtt gattaatctg atgctggaat
    42481 tacaggaaaa acagggcatt tcgtatattt atgttaccca gcatatcgga atgatgaagc
    42541 acattagcga ccaggtgctg gtgatgcatc agggcgaggt tgtcgagcga ggcagcaccg
    42601 cggatgtgct ggcatcgccg ctgcatgaac tcaccaaacg gctgattgcc gggcactttg
    42661 gcgaagcatt gacggcggat gcgtggcgta aggaccgtta gtgttctgca cctcatcctg
    42721 acaggggacc ccccctcagg atgaggtatt cattactttt gattgtcgat tctggtgaga
    42781 gcaaccagga atgccagtgt ataacacacc gtaattgcca gactcatttt taacagtgtt
    42841 tctccgccaa atcccgacaa tggcgcggct ataccgccga agacaaacat gctcatcccc
    42901 atcaacgcag acgccgttcc agattcctgc gtccctactg cactcatagc ctctgccccg
    42961 ccaacagtgc cgatgccgct acaaaatgca atagtgaaaa ataatgccac cagtgccgga
    43021 atgggcaatt gtgtccatgc gcataagacc gtgagcaatg cacacaaaat tgccgctatc
    43081 aggccacctc gcaacaatgt catcgcatta atccgtcgcg ccagacgcga aaagatccat
    43141 gaagcaatga taagtccgat gccgttaagg ccaaacacca ggctaaattg cattggacta
    43201 aagccaaatt ccttctgcaa cacgaacgac gaagagccga tatacgcaaa caagcctgcc
    43261 agcataaaac tttgaatcag gcaaaagcgc ataaaacggc ggttctgcac cacgcttcgt
    43321 ccggttaata gcaatgatga gcccctttta ttttctggca aggtctcatt aataaataac
    43381 acgcagccca gcaacaatac ggtgctaatt tcagccatta cccagaataa agtgcgccag
    43441 tcaaaagtgc taacaatgta cccgcccagc actggcgaca acaccggtgc caggccattc
    43501 accgtcatta acagcgcaaa aaactgggtc aacgttactc cctgatattt gtcacgagca
    43561 atagaacgag agagcaccga accacccgcc cccgcgatcc cttgaataaa acgccagacc
    43621 accagccagt aaatattgtt cgttgtcgcg cacaaaatgg aagaaacaat aaacaatagc
    43681 aacgacaaga ttaacggacg tttacgccct attttgtcgc tcagagggcc aaataacagt
    43741 tgtccgacgc caagaccgat cagtgatgca gtcagcgtta attgcgttat ggtcgtggtt
    43801 gccgccagtt gctcagacat ctccggcaaa gccggcaaat aaagatcggt acacatcggg
    43861 ccgataccgg ctaaaagacc aagaatcaat gcccatgaaa gagagactct agccatatta
    43921 ttccttactt gagtgatgac atgccgccgc ccagcgactg ccagagcgtg attcggtttg
    43981 taaaatcagt cagttgcagt gccagcagct cctgctgtgc gctccacaag ctgcgctgcg
    44041 ccgtcaacac ggtcaggtaa tcaccaacac ccgtctgata gcgacgcaag ccaacatcga
    44101 ccgtttgttg ctcagcttta acatactggc gctgtgcatc cagttgctct tcaagcgtgg
    44161 tccgccgcgc cagtgcgtta gcgacatctt taaatgctct ctgaacggtt ttttcatagg
    44221 tggcaatgag tccacgcttt tgtgcttctg cgtaacgaag ctgcgccaga ttacttccgc
    44281 cagtgaacag cggtaatgtg accgatggcg taaatgacca gatctgcatt ccgtggctga
    44341 acaacgacga caatgcgtca ctgccaacac cagcgctggc agtcagcgaa atcgtcggga
    44401 aaaagttagc ccgcgccgcg ccaatatcgg cattggcgct cttcaaatta tgttccgctt
    44461 cctggatatc cgggcgacgc agcagaacgt cagatgacac tcccgcagga accagactta
    44521 tcatctgttc cggcaggctt tccagtgttc ccggtagcaa attttcctca agcgttgtac
    44581 ctgccagtaa gttcagcgca tttttatcct gcatcacttg tgtctgatag ctggcaacgc
    44641 tggcgcgcgc ttgctgataa acactcatcg cctcactgac atctgtcgct gccgccgtgc
    44701 caacctgttg ctggcgctgg ataattttca atgagttttc tgcactggtc atcgtttctt
    44761 ttgccagggc caggttgctg ttatcggccg ccagtgtcag ccaggcggtg ctgatttcag
    44821 caatcagcgt taaacgggtg ttctgggctg taaattcact ggcaagccag gtttctcgcg
    44881 ccgcccgaga taaactctga ttacgaccaa acagatctaa cgtaaaactg gacaccgtgc
    44941 cgtcagcctc ggcagtggtt tccgtaccat tcgccagcga acgactgcgg gttgaactta
    45001 aggccgcatt caccgtcggg aataacgacg cattagtttg cccatagagc gcccgtgctg
    45061 agtcaatatc agcaatcgct ttttgcacat cgcggttact gttcagtgca atagtcacca
    45121 cctgctgcaa tcggggatcg tggatcacct gctgccagtc atggctgatt gcctttccct
    45181 ggccctgtgc tcccggcaag gtcgccggga ttggggattc aggcgtgctg tagtggggat
    45241 cgagcgagac acatcccgca ctcaacagca ccagaaaaat aagagaacga cgcaacataa
    45301 cttactcctg acggcgcgat ttaccggcaa acaaacgctt caccagtaca aaaaacagag
    45361 gaacaaagaa aatagccagc aacgtagcgg tcagcgtacc gccaataatg cccgtaccaa
    45421 tggcgatgcg gctgttcgcc cctgagccgg ttgctatcgc cagcggcatt accccccgca
    45481 ataaacgcca gcgaggtcat gatgattggg cgtaaacgag tctgcgccgc gcgtaatgcc
    45541 gcacgactca aggaataacc ctctgcaacc gccgcttcag caaattccac aatcagaatg
    45601 gcgtttttcg acgacaagcc gatagtggtt aatagcgcca cctggaagta aacatcgttg
    45661 tttaaatcac gcatccaggc cgccagcgcc gcgccaagaa gccccagagg gatcaccaga
    45721 atgaccgaga acgggactga ccagctttcg tacagtgctg caaggcagag gaaaaccacc
    45781 agaatagaaa ctgcatacag actcaatgcc tgaccgctgg ccagtttctc ctgcaatgac
    45841 aaaccactcc aggcccaggt tgttcccgca ggaagactgt ttgccagttc ctccattttt
    45901 gtcattgcat cgccggaact aaagccagtc gcattttccc cctgaatttc ataggctgca
    45961 gaaccgttgt agcgcaccag tctttccggt ccatacagcc agcgggtggt cgcaaaagca
    46021 gagaatggtg tcatggcgtt atcactaccg cgcacaaacc atttacctaa gtctgacggc
    46081 gcggaacgaa attcactgtc gccctgaatg tagacttttt tcacacgacc acggtcaata
    46141 aagtcattca cataagtacc gccccacgca ctggacaggg tgtcggtgac atcattcaaa
    46201 ctcaacccta atgacaccgc tttattacta tcaatatcta cctgcaattg cggcatttgt
    46261 ggcaaatcat tggcacgaac agaatgtaac tccggacttt gattcgcttt ttctatcaac
    46321 tgattacgca tttgcagtag tgtttcgcga tcggttccac cgttagctaa caattcaaac
    46381 gtaaaaccat tgctttgccc cagcccatca acggctggcg gcgtcatcgc aaataccgtg
    46441 gcatcacgaa ttgtgcccag ctctttggtt gcccgtaggg cgatagcctg ggcggtgttt
    46501 tctgcccctt tacgttgaga ccagtttttc aacgaaacaa acgccatccc ggtgttctgt
    46561 ccgctgccgc taaaactgaa accatcaacg gtaaaaatga catcggtatt tgctttctcg
    46621 ttaatcagga accagtcaac aatctggcga ttcacttctg ctgtacgggc agcggtcgcc
    46681 ccggccggca gcgtgtactg caccatgatt tcgccctggt cttcagtggg taagaaactg
    46741 cccggcagtt tccacatcat cagggccatt ccaccaccca gtacgagata aagccccatc
    46801 gttcgagcag cacggcgcag gacataaatt acgcctcgct gatatttatc ttcagtacgg
    46861 cggtaaaagc ggtcaaatgc gccgaaaaag ccttttttat gtggcggaac atgctggagg
    46921 acggaaccgc acagggcggg agtcaaggtc aatgccacca ccacggaaag cagcattgcg
    46981 gagataatgg ttatcgaaaa ctgacgataa attacccccg tagatccccc aaagaaggcc
    47041 ataggcagga atacggctga caacaccagc gcaatggcaa ccagtgcgcc agaaatctcg
    47101 cccattgatt tttccgtggc ttcacgcgcg ggcaaccctt tgtcacgcat aatgcgttcg
    47161 acgttctcca ctaccacaat ggcatcgtcc accagcaagc cgatcgccag caccatagca
    47221 aacaacgtca gggtattaat cgaatagcca aacaacgcaa gaacgccgaa tgtgcccagc
    47281 aaaacgacgg ggacagctaa cgccggaatc agtgtggcac gcaggttttg caggaataaa
    47341 tacatcacgc aaaccaccaa tacgatggct tcaaacagcg tctgaattac gtcctctaca
    47401 gagattttaa taaattcagt gctgtctttc gggtaagcga tgtcgtatcc ctgcggcatg
    47461 ttccgctgga attcggcaat cttatccttg accagcgtcg ccgtattcag cgcattcgca
    47521 ccgggcgaaa gcattacagc aaccccggca gacgggtgac cgttaagttt gccgatagcg
    47581 gtataatctt cactgcccat ctcaacgcga gccacatctt ttatacgaac aactgcgctg
    47641 tctgactggc ttttcacgat aatatttttg aactgatcca ccgtctgcaa gcgagactgc
    47701 gcacgaaccg ttgcggtcag ttgctgagta ttcggtgaag gcaatgcccc tattttcccg
    47761 gcagtaacct gcacgttttg cgcttcaata gcactttgca cgtctgaagg cattagcgag
    47821 taagacgcca gtttggccgg gtcaagccag atgcgcatag cgtattccgc gccaaagact
    47881 tgcagactcc ccacaccttc aacacgcgcc agcgggtcct gaacgttact aaccaaccag
    47941 tcggcgatat ccgaactgga ggctttgtca gtggtgtcat acacggcagc aatcagcaaa
    48001 aagttgcttt gtgatttctc caccgtaaca cccgtttgct gcacttcgct gggtaggcgc
    48061 gactccgcct gctgaatttt attctgcacc tgaacctgtg cagtatctgg atcggtacct
    48121 tgttcaaagg tcacattaat actgaccgaa ccatcagagc tgctggttga gctgaaatag
    48181 agtaaattat caagcccagt gagttgttgc tcgatgacct gagtcacgct gttctccagc
    48241 gtttcggcag aagcaccagt ataagtggct gaaattttaa tggtcggtgg cgcaacgtca
    48301 ggatattgcg cgacgggcaa tgtgcgaata gccagaatcc ccgccaacat aatcaaaatg
    48361 gcgataaccc aggcaaagac cgggcggcga acaaaaaagc gcgaaaacat caggcgttgc
    48421 ctccattagc ctgaacttca acagccttga ccgtctgccc tgaagtgact ttggcagaac
    48481 cttcaacaat cagtcggtcg ccgctgtgca ggccgtttag caccagccat ttatcaccat
    48541 atgtttctcc cgtttcgagc gttcgctgct ctactttatt gtctttattc accaccagcg
    48601 cagttgcatt gcctttagca tcgcgcgtga cgccctgttg cggcgcgaga atagcgtctt
    48661 ccatcacgcc ttcatcgaca cgagcgcgaa caaacattcc cggtaataat tgctgttgtg
    48721 gattggggaa aatcgcccgt aatgtcaccg aaccggtaga ctcatctaca gcgacttcgg
    48781 tgagttccag acgccctttt tcgctgtagg ttgtgccatc ttccagaata agtgagacgc
    48841 tcatggtgtc actgttggtc gccagtgact gtttacgcag acgtaataaa tcgacactgg
    48901 agcgagtgag gtcgacatac attgtatcca gaccacgaat agtcgttaac gctgtatctt
    48961 gcgacgcggt caccagtgcg ccaggggtta ccgacgaaat gccaatgcgc cctgaaatcg
    49021 gtgcggttac cgtggtccag tcaagattaa tgcgcgcagt ttccagtgcg gcttttttcg
    49081 ccgctacact ggctttatct tgtgcacagg tagactgagc atcatcagca tcctgttgtg
    49141 aaacaccgtt ctctttcacc agtcgggcat aacgctgcgc tttctggcaa tcggctttta
    49201 ccagtgcctg tgcttgttgt aatgctgccc gagcttcatt ccatgcagcc tgataactgg
    49261 ccgcatcaat ctggtagagc ggctgtccag ccttgaccag atcaccttcc ttaaataagc
    49321 gtttctggat aattccccca acctgcggac gtacttcggc actgagcgca gcactggtgc
    49381 gtccggttaa ttcactgact accgagaccg gttgagattt gactgtaaca acgccgactt
    49441 ctgtttctga cggggatgaa ttgttacttt gcgtattatc acaaccagat aagagcagca
    49501 ttgccactac agatgttgct atatacttca ttattttctc cagggtgaga aataccctcc
    49561 tcattaaaat taatgaggat atgtcaggga tattcagtta taacgaaccc gcatcgcgaa
    49621 atgaatttcg caatgaaata agggggatta atatcccccg ctattggtta attctgttct
    49681 tatttattta tcaacatact aacaatatcc tgatatattt cctgcagatg ttgtggatcg
    49741 cttttttgcg gtgttaaacg acgataaacc gtaccttcca tcatggtagc aaacacttca
    49801 acgcagcaac gaatgtgctc ctcgctaaga tggggatgca ttcttttcat atgtgcacaa
    49861 ccgttagcga acattctggc gtcagcttct tcaagcattg ccataacccg cgggttgcgt
    49921 gtggcttctg cagccacttc cagcatcagc gcgtcgtcgt cctcatttaa ggatttacgc
    49981 aaggcaagga cttccgggaa gtgatcggta cgcgcgtcaa tgtccatttg ggcaatacga
    50041 aagtcgatga tacggcgaac catctcttca ataatggcgt ctttgttggc gaagtaacga
    50101 tatatctgac ccacgctaag ctgagcttcg ctggcaattt gcgacatact ggcggcatga
    50161 aacccgcagc gacgaaaaca ccgacgggcg gcgttaatga tttcatcctg acgctgtttg
    50221 tggcgtattt ccagcttgct ggtcatttct gtctcctggc ctatcacttg gtgagaatga
    50281 tcgttctcat ttttgctgat tatacgtttc tccaaagtaa tggaaagtat ttttatctta
    50341 ttcatggtgc atattctgat aatgaagaga ttgtggagat tctgtgaaga tagttgacaa
    50401 tattaagttg gggtcaattc atcagaaagg ctgctacttg ttgtcgccag ttctgttttc
    50461 atcatcaaac ttatcggagc taatacgaat agtttcatag tcagattcta taagatattt
    50521 ccccaaccta ccccttgcag gcgtcaagag tagataacgc aaaatgctct gtaccgcagc
    50581 ttctctccgg tacagaaagc gcaactatag ccacccacag ccaggttgat tataataacc
    50641 gtttatctgt tcgtactgtt tactaaaacg acgaatcgcc tgattttcag gcacaacaag
    50701 catcaacaat aaggattaaa gctatgggtt ttctttccgg taagcgcatt ctggtaaccg
    50761 gtgttgccag caaactgtcc atcgcctacg gtatcgctca ggcgatgcac cgcgaaggag
    50821 ctgaactggc attcacctac cagaacgaca aactgaaagg ccgcgtagaa gaatttgccg
    50881 ctcaattggg ttctgacatc gttctgcaat gcgatgttgc cgaagatgcc agcatcgaca
    50941 ccatgttcgc tgaactgggg aaagtttggc cgaaatttga cggtttcgta cactctattg
    51001 gttttgcacc tggcgatcag ctggatggtg actatgttaa cgccgttact cgtgaaggct
    51061 tcaaaattgc ccacgacatc agctcctaca gcttcgttgc aatggcaaaa gcttgccgct
    51121 ccatgctgaa tccgggttct gccctgctga ccctttccta ccttggcgct gagcgcgcta
    51181 tcccgaacta caacgttatg ggtctggcaa aagcgtctct ggaagcgaac gtgcgctata
    51241 tggcgaacgc gatgggtccg gaaggtgtgc gtgttaacgc catctctgct ggtccgatcc
    51301 gtactctggc ggcctccggt atcaaagact tccgcaaaat gctggctcat tgcgaagccg
    51361 ttaccccgat tcgccgtacc gttactattg aagatgtggg taactctgcg gcattcctgt
    51421 gctccgatct ctctgccggt atctccggtg aagtggtcca cgttgacggc ggtttcagca
    51481 ttgctgcaat gaacgaactc gaactgaaat aatcgttctg ttggtaaaga tgggcggcgt
    51541 tctgccgccc gttatctctg ttataccttt ctgatatttg ttatcgccga tccgtctttc
    51601 tccccttccc gccttgcgtc aggataacga tttcctttac gaccaaggag cgcccatgga
    51661 acaacgccac atcaccggca aaagccactg gtatcatgaa acgcaatcca gtactacgga
    51721 gtatgacgtt ctgcctctgg tcccggaagc cgcaaaggtc agcgatccct ttctactcga
    51781 cgtgatcctt gaaaaagaaa cgctggcccc cttcctttca tggctggacc ctgcgcgtgt
    51841 tcttgcagtg gagttgttcc ctgaccagct taccgtgacc cgttcacaga ccttcaccgc
    51901 ttatgaacgc ttgtcgacgg ccctgacggt tgctcaggtt tgcggcgtcc agcggttatg
    51961 taactactat tcggcgcgac ttacgccgct ccccgggcct gattccacca gggaaagtaa
    52021 tcatcggttg gcacaaatca cgcaatatgc ccgccaactg gctagctcgc cttctattat
    52081 cgacaaccga tcgcgccagc atctgaatga cgtcggtctt actgcctggg actgtgtgat
    52141 cattaaccaa atcattggtt ttattggctt tcaggcgcgg acaattgcga catttcaggc
    52201 ttatctcggg catccggtac gctggttacc cgggctggag atacaaaact acgccgacgc
    52261 gtcactgttt gctgatgaat cattacgctg gcgaagcagc tatgaggtgg aaaaactacc
    52321 tgaagaacac acaaaaagtt caactgcaga actttgccaa ctggccgaaa tactctctct
    52381 ccaccctatt tcactttccc ttctcgaaag gttgttaaac agcacacggg ttaatacaca
    52441 gccggataat cagcttgcgg cgttgttatg cgcgcggata aatggcagtc ctgcttgttt
    52501 tgccgcctgt atggattcat caaatgaata taaaaaaatc agcccccttc tgcgcaaggg
    52561 cgaaaatgaa attaaccaat gggctgaccg tcattctgtt gagcgcgcta ccgttcaggc
    52621 gatacaatgg ctgacccgag cacccgatcg ctttagcgcc gcccagttca gccctttact
    52681 cgaacacgaa aaatcatcaa cgcagattat taatctgctg gtatggagcg ggctgtgtgg
    52741 ctggataaat cgcttaaaaa tcgcgttggg tgagacatat taaccttgcc gcgtcagaca
    52801 gattcgcgta aaactgtcag ccgctctaat ggccaccaaa atagacaatt atgtttcagg
    52861 acaacccgct gctagcgcag cttaaacagc aactgcattc ccagacgcca cgcgctgaag
    52921 gggtggtaaa agccacagaa aaaggttttg gcttcctgga agtcgacgcg caaaaaagtt
    52981 atttcattcc gccgccgcag atgaaaaaag tcatgcatgg cgaccgaatt atcgcggtga
    53041 tccacagtga aaaagaacgt gaatccgcag agccagaaga actggttgaa ccgtttctga
    53101 ctcgtttcgt gggtaaagtt cagggcaaaa atgaccgtct ggccatcgtt cctgatcatc
    53161 cactcttaaa agacgccatt ccttgccgcg cagcccgtgg tctgaaccac gagtttaaag
    53221 aaggcgactg ggcagttgcc gaaatgcgcc gtcatccgct gaaaggcgat cgttctttct
    53281 atgctgaact gacacaatac atcacttttg gtgacgacca ctttgtaccg tggtgggtta
    53341 cccttgcgcg ccataatctg gaaaaagaag caccagacgg cgtcgctacc gaaatgctcg
    53401 atgaaggtct ggttcgtaaa gatctgaccg cgctggattt tgtcaccatc gacagtgcca
    53461 gcacagaaga tatggatgac gcccttttcg ctaaggcgtt gccggatgac aaacttcagc
    53521 tgattgtggc gattgccgat ccaaccgcgt ggattgctga aggcagcaag ctggacaaag
    53581 ccgcgaaaat tcgcgcattc accaactatc tgcctggctt caacatccct atgctgcctc
    53641 gcgagctttc tgacgatctc tgctcactgc gcgccaatga agtccgcccg gtactggcat
    53701 gccgcatgac gctctccgct gatggcacca ttgaagataa tatcgaattc tttgccgcca
    53761 ccatcgaatc caaagcgaag ctggtgtatg accaggtttc tgactggctg gagaataccg
    53821 gtgactggca gcctgaaagt gaagcaattg ccgaacaagt ccgtttgcta gcgcaaattt
    53881 gccaacgccg cggcgagtgg cgtcataacc acgcactggt gtttaaagat cgcccggatt
    53941 accgctttat tctcggtgaa aaaggtgaag tgctggatat cgtcgccgag cctcgtcgca
    54001 ttgccaaccg tatcgtcgaa gaagcgatga ttgccgctaa catttgtgca gctcgcgtac
    54061 tgcgcgataa gctcggtttt ggtatctata acgtgcatat gggctttgat ccggcgaatg
    54121 ccgacgcgct ggcagcgttg ctgaaaacgc acggtctgca tgtcgatgcc gaagaagtgc
    54181 tcacgctgga cggtttctgc aaactgcgtc gtgaactgga tgcgcaacca actggtttcc
    54241 tcgacagccg cattcgtcgc ttccagtcat ttgctgaaat tagcactgaa cccggtcctc
    54301 actttggcct cggtctggaa gcatacgcca cctggacttc gccgatccgt aaatatggcg
    54361 acatgatcaa ccaccgtctg ctgaaagcgg ttatcaaagg cgaaactgcg acgcgtccac
    54421 aggatgaaat cactgtccaa atggccgagc gtcgccgtct caatcggatg gcagaacgtg
    54481 atgttggtga ctggttatac gcacgcttcc tgaaagacaa agccgggacc gacacccgtt
    54541 tcgcagcgga aattgtcgat atcagccgtg gcggcatgcg tgttcgtttg gttgataacg
    54601 gcgctatcgc ctttattcct gcacctttct tacacgctgt gcgcgatgaa ctggtttgca
    54661 gccaggaaaa cggcaccgta caaattaaag gtgaaacggt ttataaagta actgacgtta
    54721 ttgacgtcac cattgccgaa gtccgcatgg aaacccgcag cattattgcg cgcccggtcg
    54781 cgtaatctcc tttcacggcc cattccttat gaatgggccg tttatttccc cgctctacct
    54841 tcatcatatc ccggcagttt tttaactgac tttcgtttga aaactggcgt ttttccaaaa
    54901 ctagattaca aataaatata taaaaaaaac aatgtgttta gccgccgtta atttcatgta
    54961 agtcatgttg ttccttttat ttaaccgcgg actccgctgt taaccggagg atatgcatca
    55021 tgaaaaccgt tagggagtcc acaacgttgt acaactttct cggatcgcac aatccatact
    55081 ggcggttgac ggaaagcagc gatgttttgc gcttttctac caccgaaacc acagaacctg
    55141 aacgtacgtt gcagttatct gccgaacagg ctgctcgcat cagggaaatg acggtcatca
    55201 cctccagcct gatgatgagt ctgaccgtcg atgagagcga tctttctgtg catctggtag
    55261 gacgaaaaat caataaacgg gaatgggctg gcaacgcgtc tgcatggcat gacacaccag
    55321 cagttgctcg tgatttatca cacggacttt cctttgctga gcaggtagtt tctgaagcac
    55381 attccgcaat agtgattctc gacagccggg ggaatatcca acgcttcaat cggttatgtg
    55441 aagattacac agggttgaac gaacacgacg tcattggcca aagcgtgttt aaactgttta
    55501 tgagccgtcg tgaagctgcg gcatccaggc gcaataaccg tgtatttttt cgaagcggca
    55561 atgcatatga agtcgaactg tggataccaa cacgtaaagg ccagcggctg tttctgtttc
    55621 gcaataaatt tgtccacagc ggcagtggca aaaacgagat ttttttaatc tgttccggca
    55681 ccgacattac cgaagagcgc cgcgctcagg aacgactgcg tattctggca aataccgaca
    55741 gtatcaccgg actgccgaat cgtaacgcaa tgcaggagtt aatcgatcac gctattaatc
    55801 aggcagataa caataaagtt ggggtcgtgt atcttgattt ggataatttt aaaaaggtca
    55861 acgacgccta tgggcattta tttggtgacc agttattacg cgacgtgtca ttggctattt
    55921 taagctgtct cgaacatgac caggtgttgg cgcgtccagg tggcgatgag tttctggtac
    55981 tggcatccaa cacctcacaa agcgcgctgg aagcaatggc atcacgaatt ttgacccgct
    56041 tacggctccc ctttcgcatt ggtttaattg aagtttatac cagctgttca gtaggtattg
    56101 cactctctcc cgaacatggt tcggacagcg cggctattat tcgtcacgcc gacacagcaa
    56161 tgtacacagc aaaggaaggc ggacgaggac aattttgcgt ttttacccca gaaatgaatc
    56221 aacgggtatt tgaatatctc tggctggata ccaacttgcg taaagcactg gaaaacgatc
    56281 agttggttat tcactatcaa cccaaaatca cctggcgtgg cgaagtgcgc agtctggaag
    56341 cactagtacg ttggcagtca cctgaacgtg ggttgattcc accgttggac ttcatttcct
    56401 acgcagaaga gtcagggcta attgtgcctt taggccgttg ggtgattctc gatgtcgtac
    56461 gccaggtggc gaagtggcgg gataaaggca ttaacctgcg agtggcggta aacatttctg
    56521 cacgtcagct cgccgatcaa accattttca ccgccctgaa acaggttctc caggaactca
    56581 attttgaata ctattgatgt tgaattgaca gagagttgtc tgattgagaa tgatgaactg
    56641 gcactgtctg ttattcaaca atttagccaa ctaggtgcgc aagtgcatct ggatgatttt
    56701 ggtaccgcct actcttcact ttcgcaactg gcgcgctttc cgatcgatgc catcaaactt
    56761 gaccaggttt ttgttcgaga tattcacaaa caacctgtct cgcagtcact ggttcgggca
    56821 atcgtcgctg tggcccaggc attgaatctt caggtgatcg ccgaaggtgt agagagtgca
    56881 aaggaagatg cttttttaac caagaatggg atcaatgagc ggcaaggatt tttgtttgcc
    56941 aaaccgatgc ccgccgtcgc cttcgaacgc tggtataaac gctatctgaa gcgcgcataa
    57001 gtagatgaat atatatactt agcgctatat tccgtcatta tatatcgtat tcccggatga
    57061 taattagccg ttgctgttaa acacttctct tttagcggca atttttgcat tattcatttt
    57121 cccccttttg ctataaggat gtccttgatg tctgaatttg atgcccagcg cgttgccgaa
    57181 cgtattgata ttgtgttaga tatccttgtg gcagacgatt accactctgc gatccataat
    57241 ctcgagatac ttaaagcaga actcttgcgc caggttgctg aatctacgcc agacatcccg
    57301 aaggcaccgt gggagattta acaatcgtgc cttcaggttt tcctatttga ggatgcccgg
    57361 cgcgtcagta tttttttatt tagtattata acgttataag aattacagcg atgaattccc
    57421 gacaacaaac tattctacag atggtcattg accagggtca ggttagcgta accgatctgg
    57481 caaaagccac tggagtttct gaagttacca ttcgccagga tctcaacacc ctcgaaaaac
    57541 tgagttacct ccgccgtgca catggctttg cagtttcgct tgatagtgat gacgtcgaaa
    57601 cccgtatgat gagcaactat acgctgaagc gtgaactcgc cgagtttgcc gcgtcactgg
    57661 ttcaaccggg cgaaaccatc tttatcgaaa atggcagcag caatgccctg ctcgcccgga
    57721 ctctggggga gcagaagaaa aatgtcacta tcatcacggt cagcagctac atcgcgcatt
    57781 tgctgaaaga cgcgccttgt gaagttattt tgctcggtgg cgtgtaccag aaaaaaagcg
    57841 aaagtatggt tggcccgttg acacgccagt gcatccaaca ggtacatttc agcaaagcat
    57901 ttattggtat tgatggctgg caacctgaaa ctggattcac cggtcgcgat atgatgcgta
    57961 ccgatgtggt gaatgccgtg ctggaaaaag agtgcgaagc gatagtcctg actgacagct
    58021 cgaaatttgg tgctgtacat tcatactcca tcggtcccgt tgagcgattc aatcgcgtga
    58081 ttaccgattc gaaaatacgc gccagcgatc tgatgcatct tgagcacagt aaactcaccg
    58141 ttcacgtcgt tgacatttaa ccatttccct tctctacgga tgatttgcag tttgacaaat
    58201 catccgctct aagatgattc ctggttgata attaagacta tttacctgtt attaacactc
    58261 tcaagatata aaattattat cagcgatata acaggaagtc attatcacct gcgtgatata
    58321 accctgcgcg cgagcggatt tcacggaata atttcaccag gcttattctt agctattata
    58381 gttatagaga gcttacttcc gtgaatcata aattcaggag agagtattat gtttgtaacg
    58441 agcaaaaaaa tgaccgcggc tgttctggca attaccctgg caatgtctct gagtgcctgt
    58501 tctaactggt ctaaacggga ccgcaacacc gcaatcggcg cgggtgcagg ggcattaggc
    58561 ggtgcagtac tgaccgatgg cagtacgttg ggtacattag gtggcgcagc tgtcggtggt
    58621 gttattggtc accaggtcgg taaataatcg taaataaatg tcgattatcg actcgtaatt
    58681 ataatacgct tgattctgtt cagtacgaaa taactgaata atacacacag taaaatattc
    58741 gaaagccacg gatatatccg tggcttttta tgttaaccgc ctgcgagttt taccttcatc
    58801 cctttcgctt caagtagtga ttttaataaa tcacgcttat cgccctggat ttcaataact
    58861 ccatctttaa ctgctccgcc gcagccgcat tttttcttca gttccgctgc aagttttgtc
    58921 agttcggcat catcgagatc gataccggta atcaggcaaa cgcccttacc tttacgtcca
    58981 ctggtctgac gctgaatacg caccacacca tcgcctttag agcgtactgg ggccgctttg
    59041 ggctcatcaa tacgcccggt ttctgttgag tagaccagac ggctgttgga atcactcatc
    59101 atgcactccg ctgtaaagag gcgttgatcg ctttcagcgt ctgcgctgga tctaccgatt
    59161 gcgttaccgg gcgaccaatc accatataat caacaccagc cgccaacgcc tgttctggcg
    59221 tcataatgcg gcgctggtca ccagcttcac tcccctgcgg acgaatgccc ggcgtaacca
    59281 gtttgaactc ctgaccgaat acctgtttaa agcgcacagc ttcctgagca gaacacacca
    59341 caccatcaag gccacatttt tgcgtcagtg ctgccagacg ttctgcataa tctgcaggtg
    59401 acagtgtcac gccaagatcg gccaggtcgc tggcttccat gctggtcaac actgtcacag
    59461 caatcaaaag cggtgcatct ttgccaaacg gaaccagtgc ctcacgcgct gcggtcatca
    59521 tacgcgcccc accagaggca tgaacattca ccatccacac gcctaagtca gctgcagcag
    59581 cgacagcgtg cgctgcagtg ttggggatat cgtggaattt caggtcaaga aagatatcaa
    59641 aaccacgctg ttgaagttcg cgcacaaact gtggcccaaa caatgtaaac atctctttgc
    59701 cgaccttcag acgacaatcg cgtgggtcga tcttgtcgac aaaggccagc gcggcatcac
    59761 gattatgata atcaagggca acaaccacag gagaattcgt aacagcgcgg gaagaagatg
    59821 aagcagttaa cgtcatgacc agaccttctt gatgatgggc accggaagtg gcgcgaaaca
    59881 ggtaaacggc gagcattcta cccgtggaaa taaaaaatta acagttgcga tttctcccct
    59941 ggcaggcttt ttctgcgccg ccgttgccgg agcacaatgg gacataaata actagtatgt
    60001 tgtaactaaa gtcggttttt ttaaaaaatt acaggccatc aagaccgcga atcggtttaa
    60061 tagttgacca ggcacgacaa gacggacaat gccagtagag ggtgtatgcg gtaaaaccac
    60121 atttctggca gcgataacga ggcttactac gtaccttctc gccaaccatg tcacgcagca
    60181 ccatcaggct ctctttggca cgcccttctt ccgcttcatt taagtggtaa tccattaact
    60241 tatggaacac acgcatggtc ggatgacgct gaagctggcg cgtaatatag acctgtgcgg
    60301 cctcactacc gtcgcgcgct tcgatgatat cagcaagcat caattcagca tcggcaccgg
    60361 tgttctcttc caccgcgcgc tgcaggaatt ctgcccattc ggcagtttta cccaactgct
    60421 gatagcaggt ttgcagcatt tccagcgttt cgctgaccag ttctctgtcc tgggatatca
    60481 cgcgttgcaa actttcgacg gctttggcgt attctccttt cgccataaac acgcgtccca
    60541 tcatgatgga tacgcgggcg ctgtttttat ctgccgccgc cccttttttt agcaaggtca
    60601 tggcacgatc gagatcgtcg ctggccatat gctgcagggc taactcacag tagaaatggg
    60661 caatttcgac gcgctgttta tctttaccca gcttcaccag gcgttcggca acatcaattg
    60721 ctttctgcca ctcactggta gcctggtaga tttgtagcaa ctgttgcagc gcgccaatgc
    60781 ggaagtcagt ttcatcggtc agctgattga acatgtcttc cgcgcggtca tataacccgg
    60841 cggccatgta atcacgcccc agttgttgaa tcgccaacag acgctgttca taggtcagcg
    60901 aggcgctttc cattagggtc tgatggatgc gaatagcgcg atcaacttcg ccacgcgaac
    60961 ggaacaggtt tccgagcgta aggtgggctt caacggtgcc tgtatcctct ttaagcatat
    61021 cgagaaacag gtctaccgct ttatcctgtt gattactaag caggaagtta acccccgcta
    61081 cgtaatcacg cgacaagcgg ttggcttcat cttgcttgtt ttgttgcgca cttctgcggc
    61141 ccatatacca gccataggca gcggctacag gcaaaagcag aaacaacaac tccagcatag
    61201 aaagttattc cttcgccgct gacgagtgct gcactacagc cacgtcagtc gcgggtgaaa
    61261 gctggttttc cagtcgcttt attttacgtt cagcgcgcac cagggaaaca cgaactcgca
    61321 gccagaacag gccacaaatc aaccaaccga tagcaaaacc cgcagcaaac aataccgcca
    61381 gcaatgtgga aatacggtac tccccttgcg ctaacagata attaaacgtc acctgttgat
    61441 cgttctgcgc acccaacgtg accgaaatca cgaagatcgc taacaccagt aagaaaatga
    61501 gtaaatattt cacattactt cccgttatgt ggttagagcg aataaattat ggtcaactta
    61561 ccgcaatagc cctttaaagt accattttcg cgccgtctgc gaaatggcat ccacgcgcat
    61621 tgttgatgat ttaagggcta aattcggaaa atcaaccagc gttaactttc ttgttctcgt
    61681 tgcgctattt cgcgattttc ttccgcaggt ggtgttaatg gcccgcaaat tcgttgcgca
    61741 agccacgttg ccaccgccac cagtgcccac gaaatcaacg tagctaccac cagatcgcgt
    61801 ggccaatgca tcccgagcag caggcggctt cccatgactc ccgttgccca gaccagcaag
    61861 atagcaatgg ttaacgttcg ccgacgcggc cacagcaaac caacggccag cagtgcccaa
    61921 ctggcagcaa acatcgtgtg accggaagga aaggcaaacc ccgtctcttt ctgccagtgt
    61981 gaacgcaaat attgtggaat atttttctct tcagccaact gttctttcac tagatttccg
    62041 cgttctgctc gctttaaagt gtagaactca tcaaccggaa tatgatgtgt tttttccagc
    62101 cagataacaa aaggtcgtgg ttcctggact ttgtctttga tccaggattt aacgccttgt
    62161 cccacaagga ttgcggccgc cagaatggca aataatacaa aggcagcctt aatgcgaaaa
    62221 cgcagacacc agagaaacca gccgaataaa atcaaatgtg taatgacgcc ccagggctgg
    62281 gtgacagttt cagtaaccca aaaagccgct tttagtagcc aactttgttc tccaggttgc
    62341 caacgccagc cagaaatcca tacggctact ggcataacaa gcaatagtgc agctcccact
    62401 gcggtacgtc ttgcaatcga acgcatggcc tctccttttt gataagtccc acaatcataa
    62461 ctgaaaatgc cagttccagg aaaaattgac agatttgtgc cattccgtga acgatcgacg
    62521 cgtcgtgatt aggtgaaccc cttctcgtta tggcaaaata agccaataca gaaccagcat
    62581 tatctggaga atttcatgca gcttaaacgt gtggcagaag ccaaactgcc aaccccatgg
    62641 ggcgatttcc tgatggtggg atttgaagaa ctggcaaccg gacacgatca tgtcgcgcta
    62701 gtctatggcg atatttccgg gcataccccg gtacttgcgc gcgtccattc cgaatgtctg
    62761 accggtgacg ccctgttcag cttgcgctgc gattgtggct tccagctcga agcggcattg
    62821 acgcaaattg ccgaggaagg ccgtggtatt ttgctgtatc accgtcagga aggtcgtaac
    62881 attggtctgc tgaataaaat ccgcgcttac gcactgcagg atcaaggtta cgatactgta
    62941 gaggctaacc accagttagg cttcgccgct gatgagcgcg acttcactct ttgcgctgat
    63001 atgttcaaac tccttggcgt caatgaagtc cgcttgttaa ccaataaccc gaaaaaagtc
    63061 gaaattctga ccgaagcggg gattaatatt gttgaacgcg taccattgat tgtaggtcgt
    63121 aaccccaata acgaacatta tctcgatacc aaagccgaga aaatgggcca tttgctgaac
    63181 aaataaccct cttgcattgt gtaattaatt tgcttgccgg aagcaaaata accggcaagc
    63241 aaatacttgt tacttcaaca tattacgaat gacataatgc aaaatgccgt cgttctggta
    63301 gtaagtcaac tccgtcgcgg tgtcgatacg acaacggcag ggtacgactt cctggctacc
    63361 atccgcgcgc gtaagcgtca ccggaaccgt cgcgccgggt tgtagatttt gcagatcgcc
    63421 aatatcaatc ttctcttccc cggttagccc taacgtttta cgcgttacgc cttgcggaaa
    63481 ttccagcggc aggatgccca tgccaattaa attcgaacgg tgaattcgtt caaacgattc
    63541 ggcaatcacc acacgaatac caagcagacg cggacctttt gccgcccagt cacggctgga
    63601 gcctgatcca tactctttcc cggcaatcac cgccagcggc gtttgctcct gcttatagcg
    63661 catcgcagca tcataaatag agactacgtc gctgtcaggt aaatgccgcg tcatcccccc
    63721 ttcaacgcca ggcaccattt cattacggat gcgaatattg gcgaaggtgc cgcgcatcat
    63781 cacttcatgg ttaccacgcc gcgaaccgta ggagttaaag tcttttcgct caacaccccg
    63841 accttgtagg tatcgacccg ctgggctgtc gggcttaata ctgcccgccg gagagatatg
    63901 gtcagtggtg actgaatccc ccagcattgc gaggatccgc gcaccgtgaa tatcttccac
    63961 aggtgctggc gttgcctgca tttcatcaaa gaaaggcgat aaacgaatat aggttgaatc
    64021 ctcctgccat ccgtaggtat cggatcgtgc gacgttaatt tctttccact ctgcagtgcc
    64081 ttcaaagact tctgcgtact ctttgcggaa catttctgtg gagacttgtt ctaccgcacg
    64141 ggcaatttct tgtgccgatg gccagatatc tttcagatat attggatcgc ctttacgatc
    64201 atgaccaata ggctcagaag ccaggttgat attcatattt cccgccagcg cataggcaac
    64261 caccagcggc ggcgaggcca gccagttagt tttaaccagc ggatggatac ggccttcaaa
    64321 gttacggttg ccggacagca ctgcaccgac ggttaaatcg ccttttttga ttgccgtttc
    64381 gataggatcg ggcagcggcc cagagttacc aatacaggtg gtacaaccgt atcccacaag
    64441 gttaaacccc agttcgtcga gatacggtgt cagtttcgct tttgccagat agtcagaaac
    64501 gactttcgaa cccggtgcca gcgacgcttt gacccacggt tgccgcttga ggccaagagt
    64561 tacggctttt ttcgccagca agcctgcggc catcagcaca cttgggttag aagtgttggt
    64621 gcacgaggtt atcgcagcaa tgaccacagc gccatcaggt aactgatact gatgtccgtt
    64681 cataacataa tcgaccggct ggcgatcttt atgcgtggca ttcacttcca gttcgttact
    64741 ggcggcaaat gcttttggta catcgggcag tgcaacgcga tcctgtgggc gtttaggccc
    64801 tgccaggctc gcttcaacgt cattcatatc cagatctaac gtactggtaa aaatcggttc
    64861 atcgcccggg ttacgccaca tgccctgcgc tttggcatat ttttcgacca actcgacctg
    64921 atcttcgctg cgcccgctta aacgcatgta atcgagggtt acagcatcga ttgggaagaa
    64981 gccacaggtg gcaccatatt ctggcgacat attggcaatg gtggcgcgat ccgccaacgg
    65041 tagtgaatcc agaccatcac cataaaattc gacgaatttc cccaccacgc catgtttgcg
    65101 cagcatttgg gtaacagtga gaaccaggtc tgtggcggta ataccttcac gtaattttcc
    65161 ggtaagttta aagcccacta cgtccgggat aagcatggaa accggctggc ctaacattgc
    65221 ggcttctgct tcgatgccac ccacgcccca ccccagcacg ccaaggccgt tgatcatagt
    65281 ggtgtgcgag tcagtaccaa cgagtgtatc cggataagca atccattcac agtcctgcaa
    65341 ttcgctccac actgctttgc cgagatattc gaggttaacc tgatggcaaa tgcctgtgcc
    65401 tggcggcacg acgctaaacc gactgaacgc ttgctttccc catttcagga acacataacg
    65461 ttcgtggttg cgctccattt ccaggcgtac gttttcttca aatgcctcat catcaccaaa
    65521 acgatcgacg gtcaccgagt ggtcaatgac caggtcgacc ggtgagagcg ggttaacctt
    65581 tgcagtatcg ccaccgaggc gtttaaccgc ttcgcgcatt gccgccagat caacaacggc
    65641 aggtacgccg gtaaagtcct gcatcagcac ccttgccggg cggtaggcaa tttcacggtc
    65701 agcatgggca tttttcagcc atcccgccag cgcgtggata tcctcttcgg taaccgagtt
    65761 accatcctgc cagcgcagca gattttcgag caaaactttg agtgacttgg gtagacgggt
    65821 gatatcgccc agtgatttgg cagcaagcgg caggctgtag tagtggtaag ttttatcttt
    65881 ggcctgcaac gcgtccttac tggcttctcg tagggttgac gacatagctc ctccttaatg
    65941 acagggttgc gaagccctga ttctcttcag ggtgaatatt aaagataaca caaacatcgc
    66001 gtaacgattt gataacaacc caaattgata aaagaggaga aaccttgtgg caaagcaaat
    66061 gacaaccccg ccgcagcggg gtcaaggatc agtgagtaat gatccagagg agttcggccc
    66121 agaagagcat cgacagagaa aatgcaccca tccaggacca atatttcata tttgtgttga
    66181 tcataggcta cacccttaac ggttattact ttgtgttgag ataactcaac ctgttattgc
    66241 tgcggtgtat taaaagccct ttaattcatt aaaaatgggt aataataatg atgataatta
    66301 ttgtttcggc tgatcatctt cagcaaacag ttctataaag gcttgctgct gcgatgttag
    66361 ttgccagtcg tctggaataa cgacggaaca ttcccgtttc gtttcgggtt cattgtcact
    66421 atgctggcat aatggtgacg actccagttg ctcctgacca accatagtta tccccacact
    66481 ttccaaatca gcagtgcgac aaccacccag aaaagtgcag aaccaagaaa aaccgccagc
    66541 caggctttac gttttaattc aggatcgcgg cggggttcct gatttccaga aggcattgct
    66601 aacctcatat aatcgccatc acttatcagc aagacgttga acgattggtg taattaatca
    66661 tacgatgaga caaatcctaa agaaacttta gtcaaaaatc cagcgaattt acgaatcgtc
    66721 tccagtgaat aattcaatct tttgacctga aatagatatt tgaaaactgg aatttgcgag
    66781 gcgggtaatt agacactact ttacaggtaa tttaccctat aaatatgagg gataaaaata
    66841 ttgtgcacgg tatacaaata agtaacaaaa gagaatatat tccggcacct tcgctacata
    66901 aaaggtgccg aaaataacgc aagaaattat ttttccggca gttttatatc tttaaacatg
    66961 acctcaattt cttcattgga gcgcaatgcg acagccgcat caacgacatc acgcgttaaa
    67021 tgcggtgcaa aacgctgaat gaaatcatac atataactgc gcaaaaaagt actacggcga
    67081 aaaccaattt tggttgtact gtggctgaag atatcgtgag catcaacacg cacaaggtcc
    67141 ggatcggcga ccggatccac cgccatgctg gcaatgaccc ctacccccag ccctaaccgg
    67201 acgtaagttt taatgacgtc agcatccgtt gccgtgaaaa cgatacgcgg cgttaaccct
    67261 gcgcgattaa aggcagtatc cagttctgaa cgtccagtaa agccgaaggt atatgtcacc
    67321 aacggatatt gcgccagttc ttcaatggta atggcttttt tgcctgccag cgggtgatcc
    67381 ggagtgacta caatagcccg attccagtgg tagcacggta acatcactaa atcttcatac
    67441 agatgcagcg cttctgtggc gatagcgaaa tctgcattac ctttagagac ggcatcagca
    67501 atttgtgtcg gcgaaccctg gtgcatatgc aaagaaacgc ggggataacg ctcaataaag
    67561 cctttgatga cgtttggtaa cgcgtagcgt gcctgggtat gcgtggtggc gatatacagc
    67621 gagcctttat ccggccaggt gtgctcaccg gcaaccgatt ttatggcatc aactttcgac
    67681 aggacttcgc gagcgatacg aattatttct tgtcctgctg gcgttacctg cgtcaggtgc
    67741 ttgccgcttc gggaaaaaat ttgaatgcct agctcgtctt ccagcattct gacttgttta
    67801 ctgatcccgg gttgtgatgt gtaaagtcct tccgctgttg atgagacatt caggttatga
    67861 ttgaccacct caacaatata gcgaagttgt tgtaatttca tgttaaacca tccacttaga
    67921 cgccatcaga acatcgtttt ttcgtcacac ctggcgggtg actaatac
//
LOCUS       DBFIFM010000003        65303 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.003, whole genome shotgun sequence.
ACCESSION   DBFIFM010000003 DBFIFM010000000
VERSION     DBFIFM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.003"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..478)
                     /gene="folK"
                     /locus_tag="N7C74_000136"
     CDS             complement(<1..478)
                     /gene="folK"
                     /locus_tag="N7C74_000136"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000215162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HGU2430091.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILTVSSFYRTP
                     PLGPQDQPDYLNAAVALETTLAPEELLNHTQQIELQQGRVRKAERWGPRTLDLDIMLF
                     GNETINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGETLREILTSLAVLQPAIW"
     gene            complement(475..1872)
                     /gene="pcnB"
                     /locus_tag="N7C74_000137"
     CDS             complement(475..1872)
                     /gene="pcnB"
                     /locus_tag="N7C74_000137"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HGU2430092.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1932..2828)
                     /gene="gluQRS"
                     /locus_tag="N7C74_000138"
     CDS             complement(1932..2828)
                     /gene="gluQRS"
                     /locus_tag="N7C74_000138"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HGU2430093.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARHGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2895..3350)
                     /gene="dksA"
                     /locus_tag="N7C74_000139"
     CDS             complement(2895..3350)
                     /gene="dksA"
                     /locus_tag="N7C74_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HGU2430094.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3528..4232)
                     /gene="sfsA"
                     /locus_tag="N7C74_000140"
     CDS             complement(3528..4232)
                     /gene="sfsA"
                     /locus_tag="N7C74_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HGU2430095.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4247..4777)
                     /gene="thpR"
                     /locus_tag="N7C74_000141"
     CDS             complement(4247..4777)
                     /gene="thpR"
                     /locus_tag="N7C74_000141"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HGU2430096.1"
                     /translation="MSDPQRLFFAIDLPAEIREQIIHWRATHFPLEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWVLTQ"
     gene            4851..7280
                     /gene="hrpB"
                     /locus_tag="N7C74_000142"
     CDS             4851..7280
                     /gene="hrpB"
                     /locus_tag="N7C74_000142"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HGU2430097.1"
                     /translation="MSSLPVAAVLPELLTALDYAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRISQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERATAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAGKWLPEYDWPAVDDESLLATLETWLLPHMAGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7476..10010
                     /gene="mrcB"
                     /locus_tag="N7C74_000143"
     CDS             7476..10010
                     /gene="mrcB"
                     /locus_tag="N7C74_000143"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HGU2430098.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10230..12473
                     /gene="fhuA"
                     /locus_tag="N7C74_000144"
     CDS             10230..12473
                     /gene="fhuA"
                     /locus_tag="N7C74_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HGU2430099.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLDLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12524..13321
                     /gene="fhuC"
                     /locus_tag="N7C74_000145"
     CDS             12524..13321
                     /gene="fhuC"
                     /locus_tag="N7C74_000145"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HGU2430100.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13321..14211
                     /gene="fhuD"
                     /locus_tag="N7C74_000146"
     CDS             13321..14211
                     /gene="fhuD"
                     /locus_tag="N7C74_000146"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HGU2430101.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPESVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMNPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14208..16190
                     /gene="fhuB"
                     /locus_tag="N7C74_000147"
     CDS             14208..16190
                     /gene="fhuB"
                     /locus_tag="N7C74_000147"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HGU2430102.1"
                     /translation="MSKRIALFPALLLALLVIAATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGVALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16225..17505)
                     /gene="hemL"
                     /locus_tag="N7C74_000148"
     CDS             complement(16225..17505)
                     /gene="hemL"
                     /locus_tag="N7C74_000148"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HGU2430103.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17671..17718
                     /gene="yadX"
                     /locus_tag="N7C74_000149"
     CDS             17671..17718
                     /gene="yadX"
                     /locus_tag="N7C74_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HGU2430104.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17730..19151
                     /gene="clcA"
                     /locus_tag="N7C74_000150"
     CDS             17730..19151
                     /gene="clcA"
                     /locus_tag="N7C74_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HGU2430105.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19175..19240
                     /gene="yadW"
                     /locus_tag="N7C74_000151"
     CDS             19175..19240
                     /gene="yadW"
                     /locus_tag="N7C74_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HGU2430106.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19233..19577
                     /gene="erpA"
                     /locus_tag="N7C74_000152"
     CDS             19233..19577
                     /gene="erpA"
                     /locus_tag="N7C74_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HGU2430107.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19624..20247)
                     /gene="yadS"
                     /locus_tag="N7C74_000153"
     CDS             complement(19624..20247)
                     /gene="yadS"
                     /locus_tag="N7C74_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HGU2430108.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRHLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20285..21085)
                     /gene="btuF"
                     /locus_tag="N7C74_000154"
     CDS             complement(20285..21085)
                     /gene="btuF"
                     /locus_tag="N7C74_000154"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HGU2430109.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATNIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21078..21776)
                     /gene="mtnN"
                     /locus_tag="N7C74_000155"
     CDS             complement(21078..21776)
                     /gene="mtnN"
                     /locus_tag="N7C74_000155"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HGU2430110.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21860..23377
                     /gene="dgt"
                     /locus_tag="N7C74_000156"
     CDS             21860..23377
                     /gene="dgt"
                     /locus_tag="N7C74_000156"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HGU2430111.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAVL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRSPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23507..24931
                     /gene="degP"
                     /locus_tag="N7C74_000157"
     CDS             23507..24931
                     /gene="degP"
                     /locus_tag="N7C74_000157"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HGU2430112.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKLISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25086..26243
                     /gene="cdaR"
                     /locus_tag="N7C74_000158"
     CDS             25086..26243
                     /gene="cdaR"
                     /locus_tag="N7C74_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HGU2430113.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDVEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26332..26718)
                     /gene="yaeH"
                     /locus_tag="N7C74_000159"
     CDS             complement(26332..26718)
                     /gene="yaeH"
                     /locus_tag="N7C74_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HGU2430114.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27033..27857)
                     /gene="dapD"
                     /locus_tag="N7C74_000160"
     CDS             complement(27033..27857)
                     /gene="dapD"
                     /locus_tag="N7C74_000160"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HGU2430115.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGDIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27888..30560)
                     /gene="glnD"
                     /locus_tag="N7C74_000161"
     CDS             complement(27888..30560)
                     /gene="glnD"
                     /locus_tag="N7C74_000161"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HGU2430116.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVNVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEVLHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30622..31416)
                     /gene="map"
                     /locus_tag="N7C74_000162"
     CDS             complement(30622..31416)
                     /gene="map"
                     /locus_tag="N7C74_000162"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HGU2430117.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31784..32509
                     /gene="rpsB"
                     /locus_tag="N7C74_000163"
     CDS             31784..32509
                     /gene="rpsB"
                     /locus_tag="N7C74_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HGU2430118.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32767..33618
                     /gene="tsf"
                     /locus_tag="N7C74_000164"
     CDS             32767..33618
                     /gene="tsf"
                     /locus_tag="N7C74_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HGU2430119.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33765..34490
                     /gene="pyrH"
                     /locus_tag="N7C74_000165"
     CDS             33765..34490
                     /gene="pyrH"
                     /locus_tag="N7C74_000165"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HGU2430120.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34640..35197
                     /gene="frr"
                     /locus_tag="N7C74_000166"
     CDS             34640..35197
                     /gene="frr"
                     /locus_tag="N7C74_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HGU2430121.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35289..36485
                     /gene="ispC"
                     /locus_tag="N7C74_000167"
     CDS             35289..36485
                     /gene="ispC"
                     /locus_tag="N7C74_000167"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HGU2430122.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDASAREVARKE
                     VMRLAS"
     gene            36674..37432
                     /gene="ispU"
                     /locus_tag="N7C74_000168"
                     /gene_synonym="uppS"
     CDS             36674..37432
                     /gene="ispU"
                     /locus_tag="N7C74_000168"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HGU2430123.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37445..38302
                     /gene="cdsA"
                     /locus_tag="N7C74_000169"
     CDS             37445..38302
                     /gene="cdsA"
                     /locus_tag="N7C74_000169"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGU2430124.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38314..39666
                     /gene="rseP"
                     /locus_tag="N7C74_000170"
     CDS             38314..39666
                     /gene="rseP"
                     /locus_tag="N7C74_000170"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HGU2430125.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39696..42128
                     /gene="bamA"
                     /locus_tag="N7C74_000171"
     CDS             39696..42128
                     /gene="bamA"
                     /locus_tag="N7C74_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HGU2430126.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42249..42734
                     /gene="skp"
                     /locus_tag="N7C74_000172"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42249..42734
                     /gene="skp"
                     /locus_tag="N7C74_000172"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HGU2430127.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42738..43763
                     /gene="lpxD"
                     /locus_tag="N7C74_000173"
     CDS             42738..43763
                     /gene="lpxD"
                     /locus_tag="N7C74_000173"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HGU2430128.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43868..44323
                     /gene="fabZ"
                     /locus_tag="N7C74_000174"
     CDS             43868..44323
                     /gene="fabZ"
                     /locus_tag="N7C74_000174"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HGU2430129.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44327..45115
                     /gene="lpxA"
                     /locus_tag="N7C74_000175"
     CDS             44327..45115
                     /gene="lpxA"
                     /locus_tag="N7C74_000175"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HGU2430130.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMAAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45115..46263
                     /gene="lpxB"
                     /locus_tag="N7C74_000176"
     CDS             45115..46263
                     /gene="lpxB"
                     /locus_tag="N7C74_000176"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HGU2430131.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NIILEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNSARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46260..46856
                     /gene="rnhB"
                     /locus_tag="N7C74_000177"
     CDS             46260..46856
                     /gene="rnhB"
                     /locus_tag="N7C74_000177"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HGU2430132.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            46893..50375
                     /gene="dnaE"
                     /locus_tag="N7C74_000178"
     CDS             46893..50375
                     /gene="dnaE"
                     /locus_tag="N7C74_000178"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HGU2430133.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQHLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50388..51347
                     /gene="accA"
                     /locus_tag="N7C74_000179"
     CDS             50388..51347
                     /gene="accA"
                     /locus_tag="N7C74_000179"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HGU2430134.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51446..53587
                     /gene="ldcC"
                     /locus_tag="N7C74_000180"
     CDS             51446..53587
                     /gene="ldcC"
                     /locus_tag="N7C74_000180"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HGU2430135.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALCF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53644..54033
                     /gene="yaeR"
                     /locus_tag="N7C74_000181"
     CDS             53644..54033
                     /gene="yaeR"
                     /locus_tag="N7C74_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU2430136.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54098..55393
                     /gene="tilS"
                     /locus_tag="N7C74_000182"
     CDS             54098..55393
                     /gene="tilS"
                     /locus_tag="N7C74_000182"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HGU2430137.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGGAL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAETTARSAALCAE
                     QESLLDELLADDLAHCQSPQGTLQIVPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSENIVPWQTWLQPLELPA
                     GQGSVQLNAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGGFVTQEGVAEGENGISFVWQRNA"
     gene            complement(55446..55706)
                     /gene="rof"
                     /locus_tag="N7C74_000183"
     CDS             complement(55446..55706)
                     /gene="rof"
                     /locus_tag="N7C74_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HGU2430138.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55693..55893)
                     /gene="yaeP"
                     /locus_tag="N7C74_000184"
     CDS             complement(55693..55893)
                     /gene="yaeP"
                     /locus_tag="N7C74_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HGU2430139.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56059..56604
                     /gene="yaeQ"
                     /locus_tag="N7C74_000185"
     CDS             56059..56604
                     /gene="yaeQ"
                     /locus_tag="N7C74_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HGU2430140.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCAQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56601..57011
                     /gene="arfB"
                     /locus_tag="N7C74_000186"
     CDS             56601..57011
                     /gene="arfB"
                     /locus_tag="N7C74_000186"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HGU2430141.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRSGRE"
     gene            57025..57735
                     /gene="nlpE"
                     /locus_tag="N7C74_000187"
                     /gene_synonym="cutF"
     CDS             57025..57735
                     /gene="nlpE"
                     /locus_tag="N7C74_000187"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HGU2430142.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(57935..58759)
                     /gene="yaeF"
                     /locus_tag="N7C74_000188"
     CDS             complement(57935..58759)
                     /gene="yaeF"
                     /locus_tag="N7C74_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HGU2430143.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLEVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVVEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTR"
     gene            complement(58812..60530)
                     /gene="proS"
                     /locus_tag="N7C74_000189"
     CDS             complement(58812..60530)
                     /gene="proS"
                     /locus_tag="N7C74_000189"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HGU2430144.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIKK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFTAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60641..61348)
                     /gene="tsaA"
                     /locus_tag="N7C74_000190"
     CDS             complement(60641..61348)
                     /gene="tsaA"
                     /locus_tag="N7C74_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HGU2430145.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDCVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61345..61749)
                     /gene="rcsF"
                     /locus_tag="N7C74_000191"
     CDS             complement(61345..61749)
                     /gene="rcsF"
                     /locus_tag="N7C74_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HGU2430146.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQALCIGSALNITAK"
     gene            complement(61867..62682)
                     /gene="metQ"
                     /locus_tag="N7C74_000192"
     CDS             complement(61867..62682)
                     /gene="metQ"
                     /locus_tag="N7C74_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HGU2430147.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKNGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62722..63375)
                     /gene="metI"
                     /locus_tag="N7C74_000193"
     CDS             complement(62722..63375)
                     /gene="metI"
                     /locus_tag="N7C74_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HGU2430148.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVVGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63368..64399)
                     /gene="metN"
                     /locus_tag="N7C74_000194"
     CDS             complement(63368..64399)
                     /gene="metN"
                     /locus_tag="N7C74_000194"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HGU2430149.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64587..65162
                     /gene="gmhB"
                     /locus_tag="N7C74_000195"
     CDS             64587..65162
                     /gene="gmhB"
                     /locus_tag="N7C74_000195"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HGU2430150.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15381 a        16250 c        18038 g        15634 t
ORIGIN      
        1 accagatggc aggttgtagt actgctagcg aagtgagtat ttcacgtaat gtctccccat
       61 caggaaacac gagttccggg gcaatctcaa acagcggcca cagcataaag ccgcgattct
      121 tcatatcgta atgcgggacg gtgagtcgtt cggtattaat ggtttcatta ccaaacaaca
      181 tgatatcaag atcgagtgtg cgcggtcccc agcgttctgc tttacgaacg cgaccttgct
      241 gcaactctat ttgctgtgtg tgattaagta attcttcagg cgcaagtgtt gtttccagcg
      301 ccacggctgc gtttaagtaa tcgggttgat cttgcggccc cagcggtggg gtgcggtaaa
      361 acgaagaaac ggtaagaatg tggctttcag ggatatcgcc taatgctttc agggcagcat
      421 tgacctgctc cagcggagag gccagattgc tgcctatggc aatatacgcc actgtcatgc
      481 ggtaccctca cgacgtggtg cgcgtttgcg tggacgacga gtacgacgac gcggtgacgg
      541 ttcttcatcc agctcgttga gcatcccttt ttggtctggt ggcgcggaaa cctggaactc
      601 accccaccat ttcaccagac gctgcagttc agcgttacgc tcaacttcag ctcgcaaggc
      661 caacaggtca taagccgcac ggaacttagg atgctccagc aatttccatg cgcgtttacc
      721 ctgacgtcgg gacatacgca actgcaactg ccagatatcg cgggttaacg tcgtcagacg
      781 tttcgggatt gccagtgaac ggcaggcttc gtccagcacg tcgttcatcg ccagcgcgaa
      841 agcgtcgtga taggtcaggc cgctttcctg ggcgatcttc tgtgccgtct ccagcagtgg
      901 gtaccagaac atggcggcaa acaggaacgc cgggttcacg cgcatatcgt tatggatacg
      961 cgtatcggta ttcttcagca cctgttcaat gatccgctcc atcgggctgt cgccattttc
     1021 cgtgaagtag cgggtaatgg tcgggaacag cggctggaac agatgatatt cacacaacag
     1081 cttataggtt tcgtaaccgt agcccgcttg tagcagttta agcgattctt caaacaggcg
     1141 tgccggtggg atatcgttca gcagggtagc gaggcgaggg atcggttctg cggtttccgg
     1201 gctgatgcgc atacccaatt tggcggcaaa acgtactgcg cgcagcatac gtaccggatc
     1261 ttcacggtag cgcgtttccg ggttaccaat cagacggata acaccgtcct tcagatcctt
     1321 cataccgcca acgtaatcac ggacggtaaa atccgctacg ctgtaataca ggctgttgat
     1381 agtgaaatcg cggcgctggg cgtcttcttc gatggagccg aaaatgttgt cgcgcagcaa
     1441 catgccgttt tgcccgcgtt gggaggtcgt gcggtcgctg acgttacctt cgtggtgtcc
     1501 acggaaggtc gcaacttcga taatctccgg gccaaacatc acatgagcca gacggaaacg
     1561 gcgacccacc aggcggcagt tacggaacag tttgcgcacc tgctcaggcg tggcgttagt
     1621 ggttacgtca aaatctttcg gctttttgcc gagtaacagg tcgcgcacgc cgccgccaac
     1681 cagccaggct tcgtatcccg ctttattgag cctgtacatt accttcaggg cattttcact
     1741 gatatctttg cgggaaatag catgctgctc acgcgggatc accgtcacct gtggacgggc
     1801 gactgcctgt tcagcctcgc tttcctcgcg gcttagcacc ttgcggcaaa aattagcgac
     1861 tcgggtaaaa atagtacacc tcggtagtgt caaacatcat tcaggacaaa aaaatagcgg
     1921 ctaatcatag ctcagcatga cgcatttgag aatgttgaat ttacaattgc cgactcaggc
     1981 actgcggtaa gccgccagtt tttgacggct gactgaagga tttgctcgac gctgaaatcc
     2041 tgccagtgtg cttctgcctg ctgccccaga aattgaagtg ccgcgattag taccgggcgt
     2101 ggatcgcctt tcggcaacgc aggcgcatga ttctgcttgg aaagtttagc gccttgtgga
     2161 ttaagcgcca gcggcagatg aatgtaatct ggcactttcc agccaaaaag ctggtacagc
     2221 gagatttgcc gcactgtcgg ttcgatcaaa tctgccccgc ggacaatttc gctcacgccc
     2281 tggaagtgat catccaccac caccgccagg ttataggcaa acaaaccatc acggcgatga
     2341 ataataaaat cttcccgtgc cagtttttcg tcggcgtgaa taatgccgcg cagctggtca
     2401 gtaaattgcg tgaccggatg ctgctggcgg atacgcactg cggcgttgtc tggtccatga
     2461 tgcaacaccc ggcaatgacc gtcgtaaata ccgccaatgc tttgaatacg cgcacgcgtg
     2521 caggtgcagt aataacttag tccttgttca tgtaaccagg cgagtgcttc acgataggcg
     2581 tggtgacgtt gcgattgcca gagaacatcg ccgtcccagt gcagaccgta atgttccagc
     2641 tggcgcagga tagtttctgc ggcaccggga acttcacgag gcgggtcgat atcttcaatg
     2701 cgtaccagcc agcgaccgtg ccgggcgcga gcctgcaaat agctgccaag cgcagcaatc
     2761 agagaaccaa aatgaagctc gccggaagga gagggggcga agcggccaat atactgtgtg
     2821 tctgtcatct ctttgaacaa aaaataaggc gggagcattt cccgcctgtg gtaaacgtga
     2881 tggaacggct gtaattagcc agccatctgt ttttcgcgaa tttcagccag cgttttgcag
     2941 tcgatgcaca gatcggctgt cgggcgcgct tccagacggc gaataccaat ttcaacaccg
     3001 caggattcac agtagccgaa atcttcgtct tccacttttt tcagcgtctt ctcgatcttt
     3061 ttgatcagct tacgctcgcg atcgcggtta cgcagttcga ggctgaactc ttcttcctgg
     3121 gctgcacggt ctaccgggtc cgggaaattg gctgcttcat cctgcatatg tgtaacggtg
     3181 cgatcgactt catccctgag ttgattacgc catgcttcca gaatacgacg gaagtgcgcc
     3241 agctgggctt cattcatata ctcttcgccc ggcttctcct gatatggttc caccccagcg
     3301 atggcgagaa tactcaggga cgatgtttta cggttttgcc cttcttgcat gttgcttctc
     3361 cttaacacgc actatcgatc cccatgttcg ggggaaaaat gaggccgcta taaatagcag
     3421 atgcttttcc ggatagcaat tatctaaacg taacacttga caactgtgtg aggaaaagcg
     3481 tatttgcgca cgcgaccaga atgtaaatta accagttact tactttacta caatgtaacc
     3541 ggcagtgatt ttttaagagc catgccttca gcagaaattt ccgctttgta agccagaatt
     3601 tctacccccc tctgttgagc ttctgacaat agttgcgcgt atttctcatc gatgtggcgc
     3661 gcgggtgaaa accgtgtaat ggctgaatgc agcacggcga aaaagataac cgcacgctgg
     3721 ccttcagccg ctacgctcat caactcccga aggtgtttct gacctcgttc agtgaccgca
     3781 tcgggaaaat atccctgttc gttctccgct aacgtaaccg atttcacttc aatatagcag
     3841 tctggacgcg aatccgcctg caacataaag tcaatacggc tgcgttctgc gccgtatttt
     3901 acttcgcttt tcagcgagct atagcctgac agttctgaaa ttgattcatt aaggatagcc
     3961 tctttcgtca acctgttagc ccaaagcgtg ttgacgcaaa taaatgcgcc gctctggctt
     4021 tgagttaatt cccaggtgtg tgggtatttc cgtttggtgt tgtctgaagt cgaataccag
     4081 acggtatcgc caggcgttgc acaaccggtc atcgcacccg tattcgggca gtgtagcgtt
     4141 aattcgcgac catcgggtgt gatcacatcg gctaaaaaac gtttgtaacg ctgaattagc
     4201 gtcgcgcgct gtagaggggg agaaaattcc attgcgacaa tccttgttat tgcgttagca
     4261 cccagcgttt tagcggcgtg tagcgtgtgc gtccacgggc aaacgaggag gcgtaaaggg
     4321 tgaactccgt caccgcatat gaccagttaa aacctggcgg cgggattgtc accgcctcgc
     4381 tggcgtcgcg caataaggta atatgtggat gaaacggacg attgctttga aaacaaccgc
     4441 tgcgggcagc ctgtgaacgg agcatattcg ccagctggat taagccgcgt ggcggctgac
     4501 gcatccctaa ccacaccaca cgcgaacgca gccattggcc ggcgtcatca agcgtgagtg
     4561 tgaaaccagg ttgacgaatc cgtccggcta aaagagaaag cgccttctct ttctctgcgc
     4621 tcacttcgcc taaaaatgcc agagtcagat gcaaattatc ggcggcgacc ggacgtcccg
     4681 cctcaagtgg gaagtgtgtg gcgcgccagt ggataatctg ttcgcggatt tctgcaggta
     4741 agtcgatagc aaagaacaga cgttgcgggt cagacatgtg aggcactcgg ttatgaatta
     4801 ccgcgatgct acaatgtggc gcgaagaatg ttaaccctct ggagcgtttt gtgtcgtcgt
     4861 tgcccgttgc tgccgtgtta cctgaattac ttaccgctct cgattatgcg ccgcaggtat
     4921 tattaagtgc gccgaccggg gccgggaaat caacctggct gccgctgcaa ctgctggcgc
     4981 atcccggcat taacgggaaa attatcctcc tggagccacg gcgtctggcg gcgcgtaacg
     5041 tcgcgcaacg gctggcggag ctgcttaacg aaaagccagg cgataccgtt ggctaccgga
     5101 tgcgtgcgca aaactgcgtc gggccgaata cccgcctgga agtagttacc gaaggcgtgc
     5161 tgacgcggat gatccagcgt gacccggaac tgagcggtgt tggattggtg atccttgatg
     5221 aatttcatga gcgtagcttg caggcggatt tggcgttggc gctgttactc gatgtgcaac
     5281 aaggtctgcg tgatgacctt aaactgctga ttatgtcggc tacgctggac aacgaccgct
     5341 tgcagcaaat gctgccagaa gcgcccgtcg tcatctcaga agggcgctcg tttccggttg
     5401 aacgccgtta tttaccgctg cccacgcatc agcgttttga cgatgccgtt gcggtagcca
     5461 ccgctgaaat gctgcgtcag gaaagcggat cattactgtt atttttacct ggcgtcggag
     5521 aaattcagcg tgtgcaggaa caactggctt cgcgcatcgg cagtgatgta ttgctctgcc
     5581 cgctgtatgg cgcgttgtcg ctgaacgatc agcgaaaagc gatcctcccg gcaccgcaag
     5641 ggatgcgcaa agtggtgctg gcgaccaata ttgctgaaac cagtttaacc attgaaggta
     5701 ttcgtctggt ggtggattgt gcccaggaaa gggtggcgcg ttttgatccg cgaacagggc
     5761 ttacgcggtt gattactcag cgaatcagtc aggcgtcgat gacgcagcgt gccgggcgcg
     5821 ccgggcgtct ggagccgggt atctgcctgc atttaatcgc caaagaacaa gcagaacgcg
     5881 ctaccgcgca aagtgaaccg gagatcttac aaagcgatct ttccggtttg ctgatggaat
     5941 tactgcaatg gggatgcagc gatccggcgc agatgagctg gctggatcaa ccgccaacgg
     6001 tgaatctact ggccgcgaaa cgtctgttac aaatgctggg ggcgctggac ggtgaacggc
     6061 ttagtgcgca agggcaaaaa atggcagcgc tgggtaacga tccgcgttta gcggcaatgc
     6121 tggttagcgc aaagagcgac gacgaagctg ctaccgcggc aaaaattgcc gccattctcg
     6181 aagagccgcc acggatgggc aatagtgacc tgggcgtggc gttttcgcgc aatcaaccag
     6241 cctggcagca acgtagtcag caactgttaa aacgcttaaa cgtacgcggc ggtgaggcag
     6301 acagttcgct tatcgcgccg ctacttgccg gggcgtttgc cgatcgcatt gctcgccgcc
     6361 gtgggcaaga tggacgctat caactggcaa acggcatggg ggcgatgctt gatgccgacg
     6421 acgcgctaag tcgccacgaa tggttgatcg cgccgttatt attgcagggc agcgcctcgc
     6481 cggatgcgcg gattttactg gcgttaccgg tcgatattga tgaattagta caacgctgtc
     6541 cgcagctggt gcagcaatct gacaccgtgg aatgggatga cgcgcaaggt acgctgaaag
     6601 cctggcgtcg gctgcaaatc ggtcagttga cggtgaaagt gcagccgttg gcgaaaccgt
     6661 cagaagacga gttgcatcag gcgatgctta acggcatccg tgataaaggt ttaagcgtgc
     6721 tcaactggac ggcggaagcg gaacagctac gcttgcgttt gttatgcgcc ggaaagtggt
     6781 tgccagaata tgactggcca gcggttgatg atgaaagttt gttggcgacg ctggaaacgt
     6841 ggctgctgcc acatatggcc ggtgtacatt cactacgcgg cctgaaatca ctcgacattt
     6901 atcaggcact acgcggatta cttgattggg gaatgcagca acgtctggat agtgaattgc
     6961 ctgcgcatta cactgtgccg acgggaagcc ggatcgccat tcgttatcat gaagataacc
     7021 cgcccgcgct ggcggtgaga atgcaggaga tgtttggtga ggccactaat ccgacgatcg
     7081 cccaggggcg cgtgccgctg gtgctggaat tgctttcacc cgcccaaagg ccgctgcaaa
     7141 tcacgcgtga tttgggggcc ttctggaaag gggcgtatcg tgaggtgcaa aaggagatga
     7201 aagggcgtta tcccaaacac gtctggccgg acgacccggc aaacacggca ccgacgcgac
     7261 ggacgaaaaa gtattcgtaa ggttggtttt ctccctctcc ctgtgggaga gggccggggt
     7321 gagggcacca gcgcgcacgt tcaccctcac cctaaccctc tcccttaagg gagaggggac
     7381 cgatcgagct caaattttga gagatatctt cttctgtctt gtaacagaag aacagaaaat
     7441 cgggcttttg cgcctgaata ttgcggagaa aaagcatggc cgggaatgac cgcgagccaa
     7501 ttggacgcaa agggaaaccg acgcgtccgg tcaaacaaaa ggtaagccgt cgtcgttacg
     7561 aagatgacga tgattacgac gattatgatg actatgagga tgaagaaccg atgccgcgca
     7621 aaggtaaggg caaaggcaaa gggcgtaagc ctcgtggcaa acgcggctgg ctatggctgc
     7681 tgttaaaact ggctatcgtt tttgccgtgc tgatcgccat ttacggcgtt tatctcgatc
     7741 aaaaaattcg tagccgtatt gatggcaagg tctggcaact gcctgcggca gtttatggtc
     7801 gaatggtcaa ccttgagcca gacatgacca tcagcaagaa cgagatggtg aagctgctgg
     7861 aggcaacaca gtatcgtcag gtgtcgaaaa tgacccgtcc tggcgaattt accgtgcagg
     7921 ccaacagcat tgagatgatt cgccgtccgt ttgatttccc ggacagtaaa gaagggcagg
     7981 tgcgcgcgcg tctgaccttt gatggcgatc atctggcgac catcgtcaat atggagaaca
     8041 accgtcagtt cggtttcttc cgtcttgatc cgcgtctgat caccatgatc tcctcaccaa
     8101 acggtgagca gcgtttgttt gtgccgcgca gtggtttccc ggatttgctg gtggatactt
     8161 tgctggcgac agaagaccgc catttctacg agcatgatgg aatcagtctc tactccatcg
     8221 gacgtgcggt gctggcaaac ctgaccgcag gtcgcacggt gcagggggcg agtacgctga
     8281 cgcaacagct ggtgaaaaac ctgttcctct ccagcgagcg ctcttattgg cgtaaagcga
     8341 atgaagccta catggcgctg atcatggacg cgcgttacag caaagaccgt attcttgagc
     8401 tgtatatgaa cgaggtgtat ctcggtcaga gcggcgacaa cgaaatccgc ggcttcccgc
     8461 tggcgagctt gtattacttt ggtcgcccgg tagaagaatt gagcctcgat cagcaggcgc
     8521 tgttagtcgg tatggtgaaa ggagcgtcca tctacaaccc gtggcgtaac ccgaaactgg
     8581 cgctggagcg acgtaatctg gtactgcgtc tgctgcaaca gcaacagatt attgatcaag
     8641 aactctatga catgttgagt gcgcgtccgc tgggggttca gccgcgcggt ggggtgatct
     8701 ctcctcagcc agcctttatg caactggtgc gtcaggagct tcaggctaaa ttaggcgata
     8761 aggtaaaaga tctctccggc gtgaagatct tcactacctt tgactcggtg gcccaggacg
     8821 cggcagaaaa agccgccgtg gaaggtattc cggcactgaa gaaacagcgt aagttgagcg
     8881 atcttgaaac tgcgattgtg gtcgtcgacc gctttagtgg tgaagttcgt gcgatggtcg
     8941 gaggttctga gccgcagttt gcgggctaca accgtgcgat gcaggcgcgt cgttcgattg
     9001 gttcccttgc aaaaccagcg acttatctga cggccttaag ccagccgaaa atctatcgtc
     9061 tgaatacgtg gattgcggat gcgccaattg cgctgcgtca gccgaatggc caggtctggt
     9121 caccgcagaa tgatgaccgt cgttatagcg aaagcggcag agtgatgctg gtggatgcgt
     9181 tgacccgttc gatgaacgtg ccgacggtaa atctggggat ggcgctgggg ctgcctgcgg
     9241 ttacggatac ctggattaaa ctgggcgtac cgaaagatca gttgcatccg gttccggcaa
     9301 tgctgctggg ggcgttgaac ttaacgccaa tcgaagtggc gcaggcattc cagaccatcg
     9361 ccagcggtgg taaccgtgca ccgctttctg cgctgcgttc ggtaatcgcg gaagatggca
     9421 aagtgctgta tcagagcttc ccgcaggcgg aacgcgctgt tccggcgcag gcggcgtatc
     9481 tgacactatg gaccatgcag caggtggtac aacgcggtac gggtcgtcag cttggggcga
     9541 aatacccgaa cctgcatctg gcagggaaaa cagggactac caacaataac gtagatacct
     9601 ggtttgcggg cattgacggc agcacggtga ccatcacctg ggtcggccgt gataacaacc
     9661 agccgaccaa actgtatgga gccagcgggg caatgtcgat ttatcagcgt tatctggcta
     9721 accagacgcc aacgccgctg aatcttgttc cgccagaaga tattgcagat atgggcgtgg
     9781 actacgacgg caactttgtt tgcagcggtg gcatgcgtgt cttgccggtc tggaccagcg
     9841 atccgcaatc gctgtgccag cagagtgaga tgcagcagca gccgtcaggc aatccgtttg
     9901 atcagtcttc tcagccgcag caacagccgc aacagcaacc tgctcagcaa gagcagaaag
     9961 acagcgacgg tgtagccggt tggattaagg atatgtttgg tagtaattaa catctaagcg
    10021 tgaaataccg gatggcgagt tgccatccgg taaaataaca tcccacccaa agtattaacc
    10081 ctttcttttc atctggttgt ttattaaccc ttcaggaacg ctcagattgc gtaccgcttg
    10141 cgaacccgcc agagtttcga atattatctt atctttataa taatcattct cgtttacgtt
    10201 atcattcact ttacatcaga gatataccaa tggcgcgttc caaaactgct cagccaaaac
    10261 actcactgcg taaaatcgca gttgtagtag ccacagcggt tagcggcatg tctgtttatg
    10321 cacaggcagc ggttgaaccg aaagaagaca ctatcaccgt taccgctgca cctgcgccgc
    10381 aagaaagcgc atgggggcct gctgcaacta ttgcggcgcg acagtcagct accggcacta
    10441 aaaccgatac gccgattcaa aaagtgccac agtctatttc tgttgtgacc gccgaagaga
    10501 tggcgctgca tcagccgaag tcggtaaaag aagcgcttag ctacacgccg ggtgtctctg
    10561 ttggtacgcg tggcgcatcc aacacctatg accacctgat cattcgcggt tttgcggcag
    10621 aaggccaaag ccagaataac tatctgaatg gcctgaagtt gcagggcaac ttctataacg
    10681 atgcggtcat tgatccgtat atgctggaac gcgctgaaat tatgcgtggc ccggtttccg
    10741 tgctttacgg taaaagcagt cctggcggcc tgttgaatat ggtcagcaag cgtccgacca
    10801 ccgaaccgct gaaagaagtt cagtttaaag ccggtactga cagcctgttc cagactggtt
    10861 ttgactttag cgatgcgctg gatgatgacg gcgtttactc ttatcgcctg accggtcttg
    10921 cgcgttctgc caatgcccag cagaaagggt cagaagagca gcgttatgct attgcaccgg
    10981 cgttcacctg gcgtccggat gataaaacca atttcacctt cctttcttac ttccagaacg
    11041 agccggaaac cggttattac ggctggttgc cgaaagaggg aaccgttgag ccgctgccga
    11101 acggtaagcg tctgccgaca gactttaacg aaggggcgaa gaacaacacc tattctcgta
    11161 atgagaagat ggtgggctac agcttcgatc acgaatttaa cgacaccttt actgtgcgtc
    11221 agaacctgcg ctttgctgaa aacaaaacct cgcaaaacag cgtttatggt tacggcgtct
    11281 gctccgatcc ggcgaatgct tacagcaaac agtgtgcggc attagcgcca gcggataaag
    11341 gccattatct ggcacgtaaa tacgtcgttg atgatgagaa gctgcaaaac ttctccgttg
    11401 atacccagtt gcagagcaag tttgccactg gcgatatcga ccacaccctg ctgaccggtg
    11461 tcgactttat gcgtatgcgt aatgacatca acgcctggtt tggttacgac gactccgtac
    11521 cgctgctcga tctgtacaat ccggtgaata ccgatttcga cttcaatgcc aaagatccgg
    11581 caaactccgg cccttaccgc attctgaata agcagaaaca aacgggcgtt tatgttcagg
    11641 atcaggcgca gtgggataaa gtgctggtca ccctgggcgg tcgttatgac tgggcagatc
    11701 aagaatctct taaccgcgtt gccgggacga ccgataaacg tgatgacaaa cagtttacct
    11761 ggcgtggtgg tgttaactac ctgtttgata atggcgtaac accttacttt agctatagcg
    11821 aatcgtttga accttcttcg caagttggga aggatggtaa tattttcgca ccgtctaaag
    11881 gtaagcagta tgaagtcggc gtgaaatatg taccggaaga tcgtccgatt gtagttactg
    11941 gtgccgtgta taatctcact aaaaccaaca acctgatggc ggaccctgag ggttccttct
    12001 tctcggttga aggtggcgag atccgcgcac gtggcgtaga aatcgaagcg aaagcggcgc
    12061 tgtcggcgag tgttaacgta gtcggttctt atacttacac cgatgcggaa tacaccaccg
    12121 atactaccta taaaggcaat acgcctgcac aggtgccaaa acacatggct tcgctgtggg
    12181 ctgactatac cttctttgac ggtccgcttt caggtctgac gctgggcacc ggtggtcgtt
    12241 atactggctc cagctatggt gatccggcta actcctttaa agtgggaagt tatacggtcg
    12301 tggatgcgtt agtgcgttat gatctggcgc gagtcggcat ggcgggctcc aacgtggcgc
    12361 tgcatgttaa caacctgttc gatcgtgaat acgtcgccag ctgctttaac acttatggct
    12421 gcttctgggg cgcagaacgt caggtcgttg caaccgcaac cttccgtttc taatttctct
    12481 tttggggcac ggatttccgt gcctgtttca caagttggct gttatgcagg aatacacgaa
    12541 tcattccgat accacttttg cactgcgtaa tatctccttt cgtgtgcccg ggcgcacgct
    12601 tttgcatccg ctgtcgttaa cctttcctgc cgggaaagtg accggtctga ttggtcacaa
    12661 cggttctggt aaatccactc tgctcaaaat gcttggccgt catcagccgc cgtcggaagg
    12721 ggagattctt cttgatgccc agccgctgga aagctggagc agcaaagcgt tcgcccgcaa
    12781 agtggcttat ttgccgcagc agcttcctcc ggcagaaggg atgaccgtgc gtgaactggt
    12841 ggcgattggt cgttacccgt ggcatggcgc actagggcgc tttggtgcgg cagatcgcga
    12901 aaaagttgag gaagctatct cgctggttgg cttaaaaccg ctggcgcatc ggctggtaga
    12961 cagcctctct ggcggcgaac gtcagcgggc gtggatcgcc atgctggtgg cgcaggatag
    13021 ccgttgtctg ttgctcgacg aaccgacttc ggcgttggat atcgcccacc aggttgatgt
    13081 gctggcgctg gtgcaccgtt taagtcagga gcgtggcctg acggtcattg ccgtgctgca
    13141 cgatatcaat atggctgcgc gttactgtga ttatctggtc gccctgcgcg gcggtgaaat
    13201 gattgctcag ggaacgcctg cggaaattat gcgcggcgaa accctcgaaa tgatttatgg
    13261 catcccgatg ggtattctgc cgcatccggc gggtgctgca cctgtgagtt ttgtttattg
    13321 atgagcggct tacctcttat ttcgcgccgt cgactgttaa cggcgatggc gctttctccg
    13381 ttgttatggc agatgaatac cgcccacgcg gcggctattg acccaaatcg tattgtggcg
    13441 ctggagtggc tgccggtgga attactactg gcgctcggca tcgtgcctta tggcgtggca
    13501 gacaccatca actatcgact gtgggtcagc gaaccaccac tacctgaatc ggtgattgac
    13561 gtcggtttgc gtacagaacc taacctcgaa ctgctgaccg aaatgaaacc gtcgtttatg
    13621 gtctggtcgg cgggatatgg tccttcgcca gaaatgctgg ctcgtattgc gccggggcgt
    13681 ggatttaact tcagcgacgg caaacagccg ctggcgatgg cgcgtaaatc gctgacggaa
    13741 atggcggatt tacttaacct gcaaagcgca gcagaaacgc atttagcgca atatgaagac
    13801 tttatccgca gcatgaaccc ccgctttgtg aagcgtggtg cgcgtccgtt attgctgacg
    13861 acgcttatcg atccgcgcca tatgctggtc ttcggtccaa acagcttgtt ccaggaaatt
    13921 cttgatgagt acggcatccc aaatgcctgg caaggggaaa ccaacttctg gggcagtacc
    13981 gccgtcagca tcgatcgtct ggcagcgtat aaagacgttg atgtgctctg ttttgatcac
    14041 gacaacagca aagacatgga tgcgctaatg gcaacgccgc tgtggcaggc catgccgttt
    14101 gtccgcgccg gacgctttca gcgcgtaccc gcagtctggt tttatggtgc aactctctcg
    14161 gcaatgcact ttgtgcgcgt tctggataac gccattggag gtaaagcgtg agtaaacgta
    14221 ttgcgctttt cccggcatta ttgctggcgt tgctggtgat tgccgctacg gcgctgacct
    14281 ggatgaactt ctcgcaggcg ctgccgcgta gccagtgggc gcaggctgcc tggtcgccgg
    14341 atattgacgt catcgagcag atgatttttc actacagctt gctgccgcgt ctggcgattt
    14401 cgctgctggt gggcgcaggc ctggggctgg tgggcgtgct gtttcagcaa gtgctgcgta
    14461 atccgctggc ggaaccgacg acacttggcg ttgctacagg cgcgcaactg gggattaccg
    14521 tcactacgct ctgggcgatc ccaggggcga tggcgagtca gtttgctgcg ctggcgggcg
    14581 cttgcgttgt tggcttaatc gtctttggcg tcgcgtgggg gaaacggctt tcgccggtaa
    14641 cgctgatcct cgcggggctg gtagtgagcc tttattgtgg ggcaatcaat cagttactgg
    14701 ttatcttcca ccatgaccaa ctgcaaagca tgttcctgtg gagcaccgga acgctgacgc
    14761 aaaccgactg gggcggtgtt gagcgtttat ggccgcagtt gctgggcggt gtgatgctga
    14821 cgttgctgct acttcgtccg ttaacactga tggggcttga tgatggcgtg gcgcgcaatc
    14881 tcgggctagc cttgtcgctg gcgcgtctgg cggcgctgtc gctggctatt gtcatcagtg
    14941 cgctgctggt gaacgcggtg gggattatcg gctttatcgg gttgttcgcg ccgttgctgg
    15001 caaaaatgct gggggcgcgg cgtctgctgc cgcgactgat gctggcgtcg ttgattggtg
    15061 cgctgatcct ctggctttcc gatcaaatca tcctctggct gactcgcgtg tggatggaag
    15121 tgtccaccgg ttcggtcact gcgttgatcg gtgcgccgct gctactgtgg ctgttgccgc
    15181 gtttacgcag cattagcgcg ccggatatga aggtcaacga tcgtgtcgcg gctgaacgcc
    15241 aacatgtgct ggcgtttgcc ctcgcgggcg gcgtgctgct gttgatggct gtggtggtgg
    15301 cgctgtcgtt tggtcgtgat gcgcacggct ggacgtgggc gagcggggcg ttgctcgagg
    15361 atttgatgcc ctggcgctgg ccgcgaatta tggcggcgct gtttgcgggc gtcatgctgg
    15421 cggtggcggg ctgtattatt cagcggctga ccggaaaccc gatggcaagc ccggaagtgc
    15481 tggggattag ctctggcgcg gcgtttggcg tggtactgat gctgtttctg gtaccgggta
    15541 atgcttttgg ctggctgtta cccgcgggaa gtatcggtgc ggcggtgacg ctgttgatta
    15601 ttatgatcgc cgccgggcgc ggtggatttt ccccacaccg catgttactg gcggggatgg
    15661 cgttaagtac cgcattcacc atgcttttaa tgatgttgca ggcgagtggt gacccgcgaa
    15721 tggcgcaagt gctgacctgg atctctggtt cgacctacaa cgccaccgat gcgcaggtct
    15781 ggcgcaccgg aattgtgatg gtgattttgc tggcgattac cccgctgtgc cgccgctggc
    15841 tgaccatttt accgctgggt ggtgataccg cgcgggccgt gggggtggcg ttaacgccga
    15901 cgcgaattgc gctgctgctg ttagcggctt gcctgacggc gaccgcgacg atgaccattg
    15961 gaccgttgag ttttgttggt ttaatggcac cgcatatcgc gcggatgatg ggctttcgac
    16021 ggacgatgcc gcacatcgtg atttcggcgc tggtgggtgg tttactgctg gtgttcgctg
    16081 actggtgtgg gcggatggtg ctgtttccgt tccagatccc ggcggggctg ctgtcgacct
    16141 ttatcggcgc gccatatttt atctatttgt tgagaaagca gagccgttaa taaaatgcct
    16201 cgcccggtgg gcgaggcaaa atcatcacaa cttcgcaaac acccgacgtg cagcatcgat
    16261 ggtgttattg atatcttcca tgctgtgcgc cacagacata aagcccgctt caaacgctga
    16321 cggtgccagg taaacacctt catccagcat catatggaag aaacgcttaa aacgctcgac
    16381 gtcacaggcc atcacatcct gatagcacgt cacggactcg gcgtcggtaa agaaaatacc
    16441 gaacatgccg ccaacgtggt ttaccaccag cggaattccg gcttcttctg ccgcttccag
    16501 cagaccttct gccagacgtg ttgtcagctc atccagcgtt tcgtgaacgc ccggctgcgc
    16561 gacttcattc agacaggcga aaccggctgc catcgcaatc gggttaccgg aaagcgtacc
    16621 cgcctgatag accggacccg tcggggccag cgcatccatt acatcacgac gaccaccgaa
    16681 tgcgcctacc ggcattccac cgccgatgat tttgcccagg caagtgagat ccggttccac
    16741 accgtaataa tcctgtgcgc cagctagcgc tacgcggaaa ccggtcatta cttcatcgat
    16801 gatcagcaac gcgccaaatt cgtcgcacag cgcgcgcaga cctggcagga agtctggcag
    16861 cggtggaaca cagttcatat tgcctgccac cggctcgacg ataatacagg caatctcttg
    16921 cgggtattgc tcaaacgcgg cgcgtacaga agccagatcg ttataagtac aggttaaggt
    16981 atgtttggcg aaatctgccg gaacgcccgg cgagtttggc tggcctaacg tgagtgcgcc
    17041 agagccggct ttcaccagca ggcagtcagc gtgaccgtgg taacaacctt caaatttaat
    17101 aattttgtcg cgaccggtaa aaccacgggc caggcggatg gcgctcattg tcgcttcagt
    17161 gccggagttc accatgcgca ccatatccat ggtcgggacc agttcggtca ccagttgcgc
    17221 cattttcact tccatttcgg ttggtgcacc aaagcttaaa ccacgctcgg cggcttcaat
    17281 cacggcattg cggatcgccg gatggttatg gcccagcacc atcggccccc aggaaccgac
    17341 ataatcgata taggctttgc catcaacatc gtacagataa gcgccgtccg ctttttcgat
    17401 aaacagtgga gtgccgccca cgccagtaaa ggcgcgaaca ggggagttca caccgccagg
    17461 gatcagctcg cgcgctgcgc tgtaaagatt ttcagactta ctcatggagg gttcctgatt
    17521 cgtagaaaaa gtgaatggct gctattctat gttattcata acaagttaaa tactcgtcaa
    17581 acatcaggct gcttgtactg gtgcaatcct gatttcttag agtataaaag ttttgtgcat
    17641 ttgaaacatt acgctttgca aaggattttc atggaacgtg cgagtaaaat gccgtcatct
    17701 tatttgtatg accaataagt gatcattgga tgaaaactga tactccctct ttagaaacac
    17761 cgcaggccgc gcgcctgcga cgcagacaac tgattcgcca acttcttgag cgcgataaaa
    17821 ccccgttagc cattttgttt atggcggcag tcgtcggcac gcttgtcggg ctggcagcgg
    17881 ttgcttttga caaaggtgtc gcctggttgc agaaccaacg tatgggggcg ctggtacata
    17941 ctgctgataa ttatccgcta ctgttaaccg ttgcttttct ctgttcggcg gtgctggcga
    18001 tgttcggcta ctttttggtg cgcaaatacg cgcccgaagc aggggggtcg gggatcccgg
    18061 aaattgaagg ggcgctggaa gatcaacgtc ctgttcgctg gtggcgtgta ttgccggtga
    18121 aattctttgg cgggctgggg acactcggcg gaggcatggt gttggggcgc gaagggccaa
    18181 ccgtgcagat cggcggtaat atcgggcgga tggtactcga tgttttccgc ctgaaaggtg
    18241 atgaagctcg ccatacgctg ttggcaaccg gtgcggcggc gggtctggcg gcggccttta
    18301 acgcgccgct ggcgggtatt ttgtttatta tcgaagagat gcgtccgcag tttcgctata
    18361 cgttaatttc gattaaagcg gtatttattg gcgtcattat gtcgaccatt atgtaccgga
    18421 tttttaatca tgaagttgcg ttgattgacg tcggtaaact ttctgacgcg ccgcttaata
    18481 cgctgtggct ttatctgatc ctcggtatta tttttggcat tttcggccct atttttaata
    18541 aatgggtgct ggggatgcag gatttgctgc accgtgtgca cggcggcaat attaccaaat
    18601 gggtactgat gggtggtgcg attggcggtc tgtgtggatt gctggggttt gtggcaccag
    18661 caacgtcggg cggcggtttt aacctgattc ctatcgctac cgcggggaat ttcagcatgg
    18721 gaatgctggt gtttatcttc gtcgcgcggg tcattaccac cttactctgc ttctcttccg
    18781 gcgcgccggg cggtattttt gccccgatgc tggcgctggg tactgtgctg ggaaccgctt
    18841 tcggaatggt tgccgttgag ctgtttccgc aatatcacct tgaggcgggg acgtttgcta
    18901 ttgccggaat gggggcgtta ctggcggcat ctattcgcgc gccgttaacg gggatcattc
    18961 tggttctgga gatgaccgat aactaccagc tcattttgcc aatgattatt accggtcttg
    19021 gcgcaacact attagcgcaa tttaccggcg ggaaaccgct atactcggcg attcttgcgc
    19081 gcacgctggc aaaacaggaa gctgagcaac tggcgcgaag caaggccgca tcagccagcg
    19141 agaatacttg aacgaaatac cagggtatta gataatggcg attattattg ggttagaatt
    19201 tgcccaattg ccgatgtcgt ttggagcaaa atatgagtga tgacgtagca ctgccgctgg
    19261 agtttaccga cgcagcagcc aacaaagtta aaagcctgat cgctgacgaa gataacccga
    19321 atctgaaatt acgcgtgtat atcaccggtg gcggttgcag cggcttccag tatggtttca
    19381 cctttgatga tcaggtgaac gaaggcgata tgaccatcga aaaacagggc gttggcctgg
    19441 tggttgatcc gatgagcctg caatatctgg tcggcggttc cgttgactat accgaaggac
    19501 tggaaggttc tcgtttcatc gtgaccaacc cgaacgcgaa aagcacctgc ggttgcggtt
    19561 cttcctttag tatctaatct gttgttcatg tgccggataa actatccggc acatcttctc
    19621 tcttcaacgc ccattctcat ccagcgcaaa cgtcggtagc ttaagatgcc aacgaatagc
    19681 cgccagccga atcaatagcg tcacgaccat gcccatcata ctggctgttt ccagtggtac
    19741 ggaaaatgtg taataagccg tagcgtggac aataccgccg ataatacagg cagttgcgta
    19801 gatttctgta cgtaaaatca tggggatttc gcgggccaga acatcacgaa tgatcccgcc
    19861 gccaacgcca gtaatgacgc ccatacaaac cgcgattaac ggaccggctt ccgcattaaa
    19921 ggctttattc acgccaatgc cgacaaacac cgccagacca acggcgtcca gcaccggcaa
    19981 catccatttt ggtaagtgtc ttggctggcg caccagcacg atggtcagca tgctggttac
    20041 cattgcaacg accagatcgg tgggatcttt cacccaaaat accgggccgt gatccagcgc
    20101 catgtcgcga attgtcccgc cgcctacagc ggtaaccacg cccagtacca gaacaccaaa
    20161 agggtccata cgcaattttc cggctaacaa aacgccggag atggcaaata ccgctgtgcc
    20221 gactatatcc agccaataga cgagcattgt tcaatcccca ctgagcacct aaaaaggtca
    20281 ggcgctaatc tacctgtgaa agcgcattac agagctgttg tgcagcgagg ataatacgtg
    20341 ggcttgcacg ttcaaaccag tcactcgtga gaggaataac gggaattttg agctgttcac
    20401 cccagtattg tttgatttta ggaatttggt ccggtccgcc tgtaatgaca atcgcctgtg
    20461 gcgagcgtgc taacacctgt tcgcggctaa cttgcggcca gggaacccgg ctgtctttaa
    20521 agatgttttc tccgccacaa acttcgagta cctggttttg aatcgactct tttccactgg
    20581 taaatggcgg attaatgccg aattgcagaa aaacacgttt tttaggttta tcagcatatt
    20641 gcgctttcaa ttgcgcgtat tgatccagca gggattgcgc ggcttgttcg gccttatccg
    20701 gttgcggact ccagggagcc agttgacgta acgcattggc aatttgttca atgtttgtcg
    20761 catcgaccca catcactttt attcccagcg aagccagctg gtcaacctgc cgctcggcat
    20821 tacctccacg ccaggcaatc accagatcgg gtttcagcgc gacaatgcgt tccagattca
    20881 tcccctgcca ggtggaaacc tgctcaatct tttgcgcttg tggaggatag tcggaatagc
    20941 tgctgacccc aaccggcgtg atcccggcgg caaaggcaag ttcagtgttg gcgggagaaa
    21001 gcgtgatgac gcgcggcgcg gcgttgagcc acagtggcgc aagaaaagac agggcgacca
    21061 gcgccctgaa cagtgactta gccatgtgcc agtttctgca ctagtgactc aaccatcagg
    21121 ctggactgtt tagcggcaac agccaggaac tcatcgaagc taagatgaga ctgttgatcg
    21181 gccacgtcgg agatggcgcg tacgacaaca aacgggacgt tgaaattgtg gcagacatgg
    21241 gcgattgccg tcgcttccat ctctacagca atggcctgtg ggaagttgtg gcggattttc
    21301 gccagaccaa cagaaccgtt gatgaaagcg tcgccgctaa caatcaggcc acgtacagcg
    21361 ttaagattca gttcggcaat gcaggcctca gcggcagcga tcagtttatc gtcagcttta
    21421 aagcctgccg gacagcctgg taactgaccg tattcataac caaatgccgt gacatccgcg
    21481 tcgtgataac gcgcttcgtc cgagacaacg atatcgccca ctttcagcgt tggtgccagg
    21541 ccaccggcag aaccggtgtt aataatcaca tctggcttgc agtgttccaa cagcagagtg
    21601 gcacccagcg ccgcagcgac tttaccgatg cccgatttca gaagcgcaac ctcggttcca
    21661 ttcagttggc cggtatagat ttcgcaaccg cccagactga tagtttgacg gttttcgatt
    21721 ttgtcacgca gcagcgtaac ttcttcttcc attgcaccaa tgatgccgat tttcatagat
    21781 ttactcgcga taagcccgat ttgaaggcat agtttaccat gcgcttacgg ggaagcgtat
    21841 ttctcacgcg ggagaggaca tggcacagat tgatttccga aaaaaaataa actggcatcg
    21901 tcgttaccgt tcaccgcagg gcgttaaaac cgaacatgag atcctgcgga tcttcgagag
    21961 cgatcgcggg cgtatcatca actctccggc aattcgtcgt ctgcaacaaa agacccaggt
    22021 ttttccactg gagcgcaatg ccgccgtgcg cacgcgtctt acccactcga tggaagtcca
    22081 gcaggtgggg cgctacatcg ccaaagaaat tttaagccgt ctgaaagagc ttaaattgct
    22141 ggaagcatac ggcctggatg aactgaccgg accttttgaa agcattgttg agatgtcatg
    22201 cctgatgcac gatatcggca atccgccgtt tggtcatttt ggcgaagcgg cgataaatga
    22261 ctggtttcgc cagcgtttgc acccggaaga tgccgaaagc cagcctctga ctgacgatcg
    22321 ctgcagcgtg gcggtactac gtttacggga cggggaagaa ccgcttaacg agctgcggcg
    22381 caagattcgt caggacttat gtcattttga ggggaatgca caaggcattc gtctggtgca
    22441 tacattgatg cggatgaatc tcacctgggc gcaggttggc ggtattttaa aatatacccg
    22501 tccagcgtgg tggcgtggcg aaacgcctga gacacatcac tatttaatga aaaagccggg
    22561 ttattatctt tctgaagaag cctatattgc ccggttgcgt aaagaactta atttggcgct
    22621 ttacagtcgt tttccattaa cgtggattat ggaagctgcc gacgacatct cctattgtgt
    22681 ggcagatctt gaagatgcgg tagagaaaag aatatttacc gttgagcagc tttatcatca
    22741 tttgcacgag gcgtggggcc agcatgagaa aggttcgctc ttttcgctgg tggttgaaaa
    22801 tgcctgggaa aaatcacgct caaatagttt aagccgcagt acggaagatc agttttttat
    22861 gtatttacgg gtaaacaccc taaataaact ggtaccctac gcggcacaac gatttattga
    22921 taatctgcct gcgattttcg ccggaacgtt taatcatgca ttattggaag atgccagcga
    22981 atgcagcgat cttcttaagc tatataaaaa tgtcgctgta aaacatgtgt ttagccatcc
    23041 agatgtcgag cagcttgaat tgcagggcta tcgggtcatt agcggattat tagagattta
    23101 tcgcccttta ttaagcctgt cattatcaga ctttactgaa ctggtagaaa aagaacgggt
    23161 gaaacgttcc cctattgaat ctcgcttatt ccacaaactc tcgacgcgcc atcggctggc
    23221 ctatgtcgag gctgtcagta aattaccgtc agattctcct gagtttccgc tatgggaata
    23281 ttattaccgt tgccgcctgc tgcaggatta tatcagcggt atgaccgacc tctatgcctg
    23341 ggatgaatac cgacgtctga tggccgtaga acaataacca ggcttttgta aagacgaaca
    23401 ataaattttt accttttgca gaaactttag ttcggaactt cgcgctataa aacgaatctg
    23461 aagaacacag caattttgcg ttatctgtta atcgagactg aaatacatga aaaaaaccac
    23521 attagcactg agtgcactgg ctctgagttt aggtttggcg ttatctccgc tctctgcaac
    23581 ggcggctgag acttcttcag caacgacagc ccagcagatg ccaagccttg caccgatgct
    23641 cgaaaaggtg atgccttcag tggtcagcat taacgtagaa ggtagcacaa ccgttaatac
    23701 gccgcgtatg ccgcgtaatt tccagcagtt cttcggtgat gattctccgt tctgccagga
    23761 aggttctccg ttccagagct ctccgttctg ccagggtggc cagggcggta atggtggcgg
    23821 ccagcaacag aaattcatgg cgctgggttc cggcgtcatc attgatgccg ataaaggcta
    23881 tgtcgtcacc aacaaccacg ttgttgataa cgcgacggtc attaaagttc aactgagcga
    23941 tggccgtaag ttcgacgcga agatggttgg caaagatccg cgctctgata tcgcgctgat
    24001 ccaaatccag aatccgaaaa acctgaccgc aattaagatg gcggattctg atgcactgcg
    24061 cgtgggtgat tacaccgtag cgattggtaa cccgtttggt ctgggcgaga cggtaacttc
    24121 cgggattgtc tctgcgctgg ggcgtagcgg cctgaatgcc gaaaactacg aaaacttcat
    24181 ccagaccgat gcagcgatca accgtggtaa ctccggtggt gcgctggtta acctgaacgg
    24241 cgaactgatc ggtatcaaca ccgcgatcct agcaccggac ggcggtaaca tcggtatcgg
    24301 ttttgctatc ccgagtaaca tggtgaaaaa cctgacctcg cagatggtgg aatacggcca
    24361 ggtgaaacgc ggtgagctgg gtattatggg gactgagctg aactccgaac tggcgaaagc
    24421 gatgaaagtt gacgcccagc gcggtgcttt cgtaagccag gttctgccta attcctccgc
    24481 tgcaaaagcg ggcattaaag cgggtgatgt gatcacctca ctgaacggta agctgatcag
    24541 cagctttgcc gcactgcgtg ctcaagtggg tactatgccg gtgggcagca aactgaccct
    24601 gggcttgctg cgcgacggta agcaggtcaa cgtgaatctg gaactgcagc agagcagcca
    24661 gaatcaggtt gattccagct ccatcttcaa cggcattgaa ggcgctgaga tgagcaacaa
    24721 aggcaaagat cagggcgtgg tagtgaacaa cgtgaaaacg ggcactccgg ctgcgcagat
    24781 cggcctgaag aaaggtgatg tgattattgg cgcgaaccag caggctgtga aaaacatcgc
    24841 tgaactgcgt aaagttctcg acagcaaacc gtctgtgctg gcactgaaca ttcagcgcgg
    24901 cgacagcacc atctacctgt taatgcagta atctccctca accccttcct gaaaacggga
    24961 agggggtctc cttacaatct gtgaacttca ccacaactcc atacatcttc atcatccttt
    25021 aggcatttgc acaatgccgt acgttacgta cttccttatg ctaagccgtg cataacggag
    25081 gacttatggc tggctggcat cttgatacca aaatggcgca ggatatcgtg gcacgtacca
    25141 tgcgcatcat cgataccaat atcaacgtaa tggatgcccg tgggcgaatt atcggcagcg
    25201 gcgatcgtga gcgtattggt gaattgcacg aaggtgcatt gctggtactt tcacagggac
    25261 gagtcgtcga tatcgatgac gcggtggcac gtcatctgca cggtgtgcgg caagggatta
    25321 atctaccgtt acggctggaa ggtgaaattg tcggcgtaat tggcctgaca ggtgaaccag
    25381 agaatctgcg taaatatggc gaactggtct gcatgacggc tgaaatgatg ctggaacagt
    25441 cgcggttgat gcacttgttg gcgcaggata gccgtttgcg ggaggaactg gtgatgaacc
    25501 tgattcaggc agaggagaat actcccgcac ttactgaatg ggcgcaacgg ctggggatcg
    25561 atctcaatca accgcgagtg gtggctattg ttgaggtcga cagcggtcag cttggtgtgg
    25621 acagcgcaat ggcggagtta caacaactgc aaaacgcgct gactacgccc gagcgtaata
    25681 atctggtggc gattgtctcg ctaaccgaaa tggtggtgtt gaaaccggcg ttgaactctt
    25741 ttgggcgctg ggatgtagaa gatcatcgta agcgagttga acaactgatt acccgcatga
    25801 aagagtacgg ccagctgcgt tttcgcgttt cactgggcaa ctattttacc ggtcctggca
    25861 gtattgcccg atcctatcgt acggcgaaaa cgacgatggt ggtgggtaaa cagcggatgc
    25921 cagaaagtcg ctgctatttt tatcaggatc tgatgttacc tgtgttactc gacagtttgc
    25981 gtggcgactg gcaggccaac gaactggcgc gaccgctggc gcggctgaaa gcgatggaca
    26041 ataacggctt gctgcgacga acgctggcgg cgtggtttcg tcacaatgtg caaccgctgg
    26101 caacgtcaaa ggcgttgttt attcatcgta ataccctgga gtatcggctt aatcgtatat
    26161 cggaactgac cgggcttgat ttgggcaatt ttgatgacag gttgctgctg tatgtggcgt
    26221 tacaactgga tgaagagcgg taggttatgc gttaaggttg aggcggcgct ggctcattcc
    26281 cctcagcccg aaggggagag gggaatgccc gagttaaccg tcaattcggg attatttacg
    26341 cgttagtttt tccagatcgg cttcaatctc gctgatctta ttggttacga ctgactccag
    26401 gtgacgtaag tcgtcgagga tcttacgctt aagatcaact tcgctgcggt cacgctggca
    26461 gatttgatca agctcatcaa tgatataccg tagattcggg ctgatttcct ggacttcttt
    26521 ataaccctga cccacaccat cagcgacgac cgttttacgc tgacgcggat atttaaactt
    26581 aacgctcttg gcgaaaaact cgcctttgtc tttctggaaa tagattttca gaatatcgtt
    26641 gttggcttcc tgccggaggc tgtaacgatc aatttcttca ggattggtaa tacccagact
    26701 tttcagattg tcgtacatag cgttacctca aaatgagtca gtaaaatgtg cttattttag
    26761 catttggcct ggcccgcccg gctcgactgt gatcgcagaa agcctttttc ttccagactt
    26821 ctatcatggc gtaaaaatca aaaaattacc tgctttattg tggcgatatt ccatgggatg
    26881 gattagcaca tttatcttcg ttttgcgaat tacatgacag tccgaaacaa gatgaaaaag
    26941 tatttaaaaa aggggggagg aagaatgagt aggacgaatt attctgaacc agaaagcaat
    27001 cgggcagcgt cgtgctgccc gtgtgcatac ttttagtcga tggtacgcag cagttcgtta
    27061 atgccgactt tgccgcgagt tttcgcgtca actttcttaa cgataaccgc acagtagagg
    27121 ctgtatttgc catctttcga cggcagatta cctgaaacaa ccacagaccc cgccggaacg
    27181 cgaccgtagt ggatgtcgcc ggtttcacgg tcgtaaatac gggtgctctg accaatgtat
    27241 acgcccatgg aaatgacgga accttcttcg acaatcaccc cttcaactac ttcagagcgc
    27301 gcgccgatga agcaattatc ttcaatgatg gttgggttag cctgcagcgg ttccagcaca
    27361 ccgccgatgc ccacgccacc ggaaaggtgg acgtttttac caatctgcgc acaagaaccg
    27421 acggtcgccc aggtatcgac catggtgcct tcatcaacat atgcgccgat gttgacgtaa
    27481 gacggcatca gcacggtgtt acgggcaata aacgcaccct gacgtaccgc cgcaggtggt
    27541 acaacgcgga agccttcttt ctggaaacgt gcttcgtcgt agtcggcgaa tttcatcggc
    27601 actttgtcga agtagcggct ttctgcccct tcgatcacct gattatcatt aatacggaaa
    27661 gagagcagca ccgctttttt caaccactga tgcgtcaccc actgaccgtc aattttttcc
    27721 gctacacgca gtgcgccgga atccagcagg gcgatcacct gatttaccgc ttcgcgggta
    27781 acggtgtctg cattggctgg cgtgatctcg gcacggcgtt caaaagcggt ttcaataatg
    27841 ttctgtaact gctgcattgt taaactcttt tcatatcagt aaacacatca ccctttatcg
    27901 tttggattga gggcctctgt caaccgctga tgcacttcct gctgcaactc gttattaagt
    27961 gcacgccggt cagcggtggc aattatgaat aaatcttcta ctcgctcgcc aatggttgta
    28021 attcgggcac catgaagcga aattcccaga tcggcaaaaa ttttcccgac tctcgccagc
    28081 agtcctggct ggtcgagcgc gataagctca aggaacgatt tgcggtcggt atgggttggc
    28141 aaaaacgtca cttcggtttc aacagtaaag tgacgtagtt tagccggttg acgacgcggc
    28201 tgtggtggct gccagctact ttgtgtcagt acttgctcca gaccaaaccg aataacctca
    28261 tgacgatctg cggatagcgg gctaccatcg ggttccagca cgataaaggt atccatcgcc
    28321 ataccgtcgc gagtggtgaa aatttgtgcg tcgtgaacgc ttaagttgcg tctatctaac
    28381 tcggcacaaa cagcagcaaa caagtaaggg cggtccgggc tccagataaa aatctcggtg
    28441 cctccacgcg tagcctgcgg gctaagcaat accagcggtt tgcttaaatc atgctgtaat
    28501 aaatggcgcg catgccaggc cagttgattt gggctatgac ggacaaaata gttcgcgcga
    28561 cagcgtgacc aaatttggtg cagcacctct tcgtcgatgt tatccatgcg cagtagtgcc
    28621 agcgcctgga gttggtgatg gcgaacccgt tcgcgcatat ccggcgtgtt ttgcattccg
    28681 cgtcgtagct gcttttcggt ggcgaaatag agttcgcgca acagactttg cttccagcta
    28741 ttccacagcg tttcgttggt ggcgcaaatg tcagccactg tcaggcatac cagatagcgc
    28801 agacgatttt ccgtttgaac ttcttcggca aactgcttga tgacttccgg gtcctgaata
    28861 tcgcgacgct gagctgtcac cgacatcagc aagtgctggc gaaccagcca ggcgaccagc
    28921 tgtgtttcgc gtgagttcag cccgtggagt tcggcaaaat gcactacatc ctgagcaccg
    28981 agaatagagt ggtcgccgcc gcgtcctttg gcgatatcgt gaaacagcgc ggcgatgaaa
    29041 atcagctcag ttgacggtag gcgcggccag acgttcacac acaacggatg gcgctggcgc
    29101 gtttcttcac tggcaaaact ctccagtttc agcatcacgc ggatagtatg ttcatccacc
    29161 gtgtaggcgt ggaacagatc aaactgcatc tgcccgacga tatgtgacca ttgcggcata
    29221 tacgcgccga gtacgctatg gcgatgcatt ggcaatagcc cgcgccgcac cgctccgggg
    29281 tgacgtagaa tgctcaaaaa cagttttcgt gcttgcggaa tattacacag cggttgttgc
    29341 agatggcgac gggcatggcg taactggcgc agtgtggtgg agtaaatgcc ggtgatcgca
    29401 ctgttgcgca ccatggtgta gaacatacgc aagatggctt ccggctggcg cataaatagt
    29461 gtttcatcac gcaggtcgat tagcgtaccg cgtagctgaa actcatcgtc gattggacgt
    29521 ggtttttcgt cggcgggaag ggcgaggatg gcttcatcga acagttgcag cagcatctgg
    29581 ttgagttcac tgacgcggcg tgtcacgcgg aagtaatcct tcatcatccg ctcgaccggt
    29641 tcgttacctt cgccactgta attcagacgc tgggcgacgc taagctggcg atcgaataac
    29701 aggcgattat cgtaacggct gacgaccaga tgcagggcaa agcgaatacg ccacaatata
    29761 tgcagacatt cgtttaattc cgcccgctcc gctgaggtta agaagccaaa cccaaccatt
    29821 tcatccagcg atgttgcgcc aaaatgacgg cgggccaccc attgcagagt gtggatatcg
    29881 cgcaagccgc cagggctact tttgatgtct ggttcaagat tgtagctggt gccgtggtaa
    29941 cgctgatggc gctggttctg ttcttcaact ttcgccgcgt agaacttgtc ggaaggccag
    30001 aatccttcgc tgaaaatatg tttttgcagt tcaagaaata gcgcaacatc gccaattaat
    30061 aagcgggatt cgatcaaatt ggtggcgacg gttaaatccg ataacccttc cagcatgcac
    30121 tcttcaagcg tgcgcacact atgaccgact tccagcttta catcccagag cagcgttaac
    30181 agctcgccca ctttttgcgc ctgatcgtcc gggagctttt tacggcttaa aatcagtaaa
    30241 tcgatgtcag aaagtggatg cagctcgcca cgaccgtagc caccgacggc gaccaatgcc
    30301 aggtcggcaa tctggctgaa tcccgcttca atccataatc gttgcaggag ctggtcgata
    30361 aactcggtgc gcgcctcaat caactgttct gcagagatcc cattgtcaaa ggcatcaccc
    30421 agccaacgct ggaaagtatc gatatgggct tttatcccac cgacggttaa ttcatcacgg
    30481 ggccagacgc atggattttg cggttgaccg ggcagggtgg ggagagcggt gtttgcgtac
    30541 tgttctggaa gggtattcat tgtgcgccac ccataagatt aaattatcgc attaaaaaag
    30601 ccggcattcg ccggcttcat tttattcgtc gtgcgagatt atcgccggga tggtgtcatc
    30661 cttgcgtagc gtcagaattt cgcagccgtt atcagtcacc acaatagtat gctcatattg
    30721 tgcagacaag ctgcgatctt tggtttttac cgtccagcca tctttcatgg tgcggatctc
    30781 ttttttaccc gcgttgacca ttggctcgat ggtgaacgtc atcccaggtt tcagtacgac
    30841 gttggtttca cgggagtcat agtgcagcac ctgcggttct tcatggaagc cgcgaccaat
    30901 accgtgtccg caatattcac gaacgacgga gaagccttct gcttcgacaa atttctgaat
    30961 cgccgcaccg atttcgcgca gattaatgcc tggttttacc atgcgtagcg ccaggtacag
    31021 gctttcttgc gtgatacggc acagacgttc gcccatgatg gtcggcttac cgacgataaa
    31081 cattttcgag gtatcgccgt ggaaaccatc tttgattacg gtgacatcaa tgttaacgat
    31141 atcgccatct ttcagcagct tagcatcgtc cggaataccg tggcacacca cttcattaat
    31201 agaaatgcaa acggatttcg gataaccgtg atagccgagg caggctgaaa ccgcgtgttg
    31261 ttcattaaca atgtaatcat tacagatgcg atccagctcg ccggtgctga cgcccggttt
    31321 aacatatggt tcgatcatct ccagcacttc ggcagccagt cggccagcga cgcgcatttt
    31381 ttcgatatct tctggggtct tgattgagat agccattaat tctgtccatc agcgtcggtg
    31441 ataccgacaa tatatatgta agtgccgtca atggtatcac acccgggcaa attgagaatc
    31501 attctgaatt tcgccaaacg tgccactgaa ggttttctat aatagaaaat tcgacgtctg
    31561 atgctgtaca cagcgccaac aattattggt gtccacggcg tatttgtggt ataaagcgcg
    31621 ccggacttcc gatccatttc gtatacacag actggacgga agcgacaatc tcactttgtg
    31681 taacaacaca cacgtatcgg cacatattcc ggggtgccct ttggggtcgg taatatggga
    31741 tacgtggagg cataacccca acttttatat agaggtttta atcatggcaa ctgtttccat
    31801 gcgcgacatg ctcaaggctg gtgttcactt cggtcaccag acccgttact ggaacccgaa
    31861 aatgaagccg ttcatcttcg gtgcgcgtaa caaagttcac atcatcaacc ttgagaaaac
    31921 tgtaccgatg ttcaacgaag ctctggctga actgaacaag attgcttctc gcaaaggtaa
    31981 aatccttttc gttggtacta aacgcgctgc aagcgaagcg gtgaaagacg ctgctctgag
    32041 ctgcgaccag ttcttcgtga accatcgctg gctgggcggt atgctgacta actggaaaac
    32101 cgttcgtcag tccatcaaac gtctgaaaga cctggaaact cagtctcagg acggtacttt
    32161 cgacaagctg accaagaaag aagcgctgat gcgcactcgt gagctggaga aactggaaaa
    32221 cagcctgggc ggtatcaaag acatgggcgg tctgccggac gctctgtttg taatcgatgc
    32281 tgaccacgaa cacattgcta tcaaagaagc aaacaacctg ggtattccgg tatttgctat
    32341 cgttgatacc aactctgatc cggacggtgt tgacttcgtt atcccgggta acgacgacgc
    32401 aatccgtgct gtgaccctgt acctgggcgc tgttgctgca accgtacgtg aaggccgttc
    32461 tcaggatctg gcttcccagg cggaagaaag cttcgtagaa gctgagtaat aaggcttgat
    32521 aactccccca aaatagttcg agttgcagaa aggcggcaag ctcgagaatt cccgggagct
    32581 tacatcagta agtgaccggg atgagcgagc gaagatgacg catctgcggc gcgaaatatg
    32641 aagggggaga gcccttatag accaggtagt acacgtttgg ttagggggcc tgcatatggc
    32701 cccctttttc acttttatat ctgtgcggtt taatgccggg cagatcacat ctccgaggat
    32761 tttagaatgg ctgaaattac cgcatccctg gtaaaagagc tgcgtgagcg tactggcgca
    32821 ggcatgatgg attgcaaaaa agcactgact gaagctaacg gcgacatcga gctggcaatc
    32881 gaaaacatgc gtaagtccgg tgctattaaa gcagcgaaaa aagcaggcaa cgttgctgct
    32941 gacggcgtga tcaaaaccaa aatcgacggc aactacggca tcattctgga agttaactgc
    33001 cagactgact tcgttgcaaa agacgctggt ttccaggcgt tcgcagacaa agttctggac
    33061 gcagctgttg ctggcaaaat cactgacgtt gaagttctga aagcacagtt cgaagaagaa
    33121 cgtgttgcgc tggtagcgaa aattggtgaa aacatcaaca ttcgccgcgt tgctgcgctg
    33181 gaaggcgacg ttctgggttc ttatcagcat ggtgcgcgta tcggcgttct ggttgctgct
    33241 aaaggcgctg acgaagagct ggttaaacac atcgctatgc acgttgctgc aagcaagcca
    33301 gaattcatca aaccggaaga cgtatccgct gaagtggtag aaaaagaata ccaggtacag
    33361 ctggatatcg cgatgcagtc tggtaagccg aaagaaatcg cagagaaaat ggttgaaggc
    33421 cgcatgaaga aattcaccgg cgaagtttct ctgaccggtc agccgttcgt tatggaacca
    33481 agcaaaactg ttggtcagct gctgaaagag cataacgctg aagtgactgg cttcatccgc
    33541 ttcgaagtgg gtgaaggcat cgagaaagtt gagactgact ttgcagcaga agttgctgcg
    33601 atgtccaagc agtcttaatt atcaaaaagg agccgcctga gggcggcttc tttttgtgcc
    33661 catcttgtaa attcagctaa cccttgtggg gctgcgctga aaagcgacgt acaatgtcgc
    33721 tagtattaat tcatttcaat cgttgacagt ctcaggaaag aaacatggct accaatgcaa
    33781 aacccgtcta taaacgcatt ctgcttaagt tgagtggcga agctctgcag ggcactgaag
    33841 gcttcggtat tgatgcaagc atactggatc gtatggctca ggaaatcaaa gaactggttg
    33901 aactgggtat tcaggttggt gtggtgattg gtgggggtaa cctgttccgt ggcgctggtc
    33961 tggcgaaagc gggtatgaac cgcgttgtgg gcgaccacat ggggatgctg gcgaccgtaa
    34021 tgaacggcct ggcaatgcgt gatgcactgc accgcgccta tgtgaacgct cgtctgatgt
    34081 ccgctattcc attgaatggc gtgtgcgaca gctacagctg ggcagaagct atcagcctgt
    34141 tgcgcaacaa ccgtgtggtg atcctctccg ccggtacagg taacccgttc tttaccaccg
    34201 actcagcagc ttgcctgcgt ggtatcgaaa ttgaagccga tgtggtgctg aaagcaacca
    34261 aagttgacgg cgtgtttacc gctgatccgg cgaaagatcc aaccgcaacc atgtacgagc
    34321 aactgactta cagcgaagtg ctggaaaaag agctgaaagt catggacctg gcggccttca
    34381 cgctggctcg tgaccataaa ttaccgattc gtgttttcaa catgaacaaa ccgggtgcgc
    34441 tgcgccgtgt ggtaatgggt gaaaaagaag ggactttaat cacggaataa ttcccgtgat
    34501 ggataaataa gggtaagatt ccgcgtaagt atcgcggggg atcttacccg taatatgttt
    34561 aatcagggct atacttagca cacttccact gtgtgtgact gtctggtctg actgagacaa
    34621 gttttcaagg attcgtaacg tgattagcga tatcagaaaa gatgctgaag tacgcatgga
    34681 caaatgcgta gaagcgttca aaacccaaat cagcaaaata cgcacgggtc gtgcttctcc
    34741 cagcctgctg gatggcattg tcgtggaata ttacggcacg ccgacgccgc tgcgtcagct
    34801 ggcaagcgta acggtagaag attcccgtac tctgaaaatc aacgtgtttg atcgttcaat
    34861 gtctccggcc gttgaaaaag cgattatggc gtccgatctt ggcctgaacc cgaactctgc
    34921 gggtagcgac atccgtgttc cgctgccgcc gctgacggaa gaacgtcgta aagatctgac
    34981 caaaatcgtt cgtggtgaag cagaacaagc gcgtgttgca gtacgtaacg tgcgtcgtga
    35041 cgcgaacgac aaagtgaaag cactgttgaa agataaagag atcagcgaag acgacgatcg
    35101 ccgttctcag gacgatgtac agaaactgac tgatgctgca atcaagaaaa ttgaagcggc
    35161 gctggcagac aaagaagcag aactgatgca gttctgattt cttgaacgac aaaaacgccg
    35221 ctcagtagat ccttgcggat cggctggcgg cgttttgctt tttattctgt ctcaactctg
    35281 gatgtttcat gaagcaactc accattctgg gctcgaccgg ctcgattggt tgcagcacgc
    35341 tggacgtggt gcgccataat cccgaacact tccgcgtagt tgcgctggtg gcaggcaaaa
    35401 atgtcactcg catggtagaa cagtgcctgg aattctctcc ccgctatgcc gtaatggacg
    35461 atgaagcgag tgcgaaactt cttaaaacga tgctacagca acagggtagc cgcaccgaag
    35521 tcttaagtgg gcaacaagcc gcttgcgata tggcagcgct tgaggatgtt gatcaggtga
    35581 tggcagccat tgttggcgct gctgggctgt tacctacgct tgctgcgatc cgcgcgggta
    35641 aaaccatttt gctggccaat aaagaatcac tggttacctg cggacgtctg tttatggacg
    35701 ccgtaaagca gagcaaagcg caattgttac cggtcgatag cgaacataac gccatttttc
    35761 agagtttacc gcaacctatc cagcataatc tgggatacgc tgaccttgag caaaatggcg
    35821 tggtgtccat tttacttacc gggtctggtg gccctttccg tgagacgcca ttgcgcgatt
    35881 tggcaacaat gacgcccgat caagcctgcc gtcatccgaa ctggtcgatg ggacgtaaaa
    35941 tttctgtcga ttcggctacc atgatgaaca aaggtctgga atacattgaa gcgcgttggc
    36001 tgtttaacgc cagcgccagc cagatggaag tgctgattca cccgcagtca gtgattcact
    36061 caatggtgcg ctatcaggac ggcagtgttc tggcgcagct gggggaaccg gatatgcgta
    36121 cgccaattgc ccacaccatg gcatggccga atcgcgtgaa ctctggcgtg aagccgctcg
    36181 atttttgcaa actaagtgcg ttgacatttg ccgcaccgga ttatgatcgt tatccatgcc
    36241 tgaaactggc gatggaggcg ttcgaacaag gccaggcagc gacgacagca ttgaatgccg
    36301 caaacgaaat caccgttgct gcttttcttg cgcaacaaat ccgctttacg gatatcgctg
    36361 cgttgaattt atccgtactg gaaaaaatgg atatgcgcga accacaatgt gtggacgatg
    36421 tgttatctgt tgatgcgagc gcgcgtgaag tcgccagaaa agaggtgatg cgtctcgcaa
    36481 gctgaggata atccggctac agagagtcgc gctatttgtt agcgtagggc ttcagtgata
    36541 tagtctgcgc catctgatcg taagtagttg gctttataag gtcaggtatg ccgtggtttt
    36601 acacggcttt tttttgtata ggcttcagta ttcctgagta ccgtaaaccc tgtcagggaa
    36661 taaaaaacgc gtgatgttgt ctgctactca accacttagc gaaaaattgc cagcgcatgg
    36721 ctgccgtcat gttgcgatca ttatggacgg caatggtcgc tgggcaaaaa agcaagggaa
    36781 gattcgtgcc tttgggcata aagccggagc aaaatccgtc cgccgggctg tctcttttgc
    36841 ggccaacaac ggtattgagg cgttaacgct gtatgccttt agtagtgaaa actggaaccg
    36901 accagcgcag gaagtcagtg cgttaatgga actgtttgtg tgggcgctcg atagcgaagt
    36961 aaaaagtctg caccgacata acgtgcgtct gcgtattatt ggcgatacca gtcgctttaa
    37021 ctcgcgtttg caagaacgta ttcgtaaatc tgaagcgcta acagccggga ataccggtct
    37081 gacgctgaat attgcggcga actacggtgg acgttgggat atagtccagg gagtcaggca
    37141 actggctgaa aaggtgcagc aaggaaacct gcaaccagat cagatagatg aagagatgct
    37201 aaaccagcat gtctgtatgc atgaactggc ccctgtagat ttagtaatta ggactggggg
    37261 ggagcatcgc attagtaact ttttgctttg gcaaattgcc tatgccgaac tttactttac
    37321 agatgttctc tggcccgatt tcgatgaaca agactttgaa ggggcgttaa atgcctttgc
    37381 taatcgagag cgtcgtttcg gcggcaccga gcccggtgat gaaacagcct gatgggggtc
    37441 gcttttgctg aagtatcgcc tgatatctgc ttttgtgtta atacccgtcg tcatcgcggc
    37501 gttgtttctg ttgccgccgg tggggttcgc cattgtaacg ctggtggtct gcatgctggc
    37561 agcgtgggaa tggggacagc ttagcggttt taccactcgt tcgcagcgag tatggttggc
    37621 ggtgttatgc gggttattgt tggcgctgat gctttttctg ttgccggaat atcaccgaaa
    37681 tattcatcaa ccgctggttg aaatctcact ttgggcttcg ctgggttggt ggattgtcgc
    37741 gctattgctg gtgctgtttt acccaggttc cgcagcaatc tggcgtaact ctaaaacatt
    37801 gcgccttatt tttggcgtgc taaccattgt tcccttcttc tggggcatgc tggcgttacg
    37861 ggcctggcac tatgacgaga atcattacag tggcgcaata tggctgctct atgtcatgat
    37921 cctggtatgg ggcgctgact ccggcgcata tatgtttggc aaattgtttg gtaaacataa
    37981 gctggcaccg aaggtttctc cgggtaaaac ctggcaaggc tttatcggtg gactcgctac
    38041 tgcagcggta atctcatggg gttatggcat gtgggcgaat ctcgacgtcg ctcccgtcac
    38101 cttactcatt tgctctattg tcgcagcgtt agcctcagtg ctcggcgatc tgaccgagag
    38161 tatgtttaag cgtgaagcag gaattaagga cagcggtcat ttaattccag gacacggtgg
    38221 tattttagat cgtattgata gcctgacggc tgcggtaccg gtctttgctt gcttgttgtt
    38281 actggtattc aggacgcttt aacggaaggt aatatgctga gttttctctg ggatttggct
    38341 tcgttcatcg ttgcactggg tgtacttatc accgtgcatg aatttggtca tttctgggtt
    38401 gcccggcgtt gtggtgttcg cgttgagcgt ttctcaatag ggtttggtaa ggcgctctgg
    38461 cggcgaactg ataagctcgg caccgaatat gttatcgccc tgatcccgtt gggcggttat
    38521 gtcaaaatgc tggatgagcg cgcagaaccg gtcgttccgg aactccgcca ccatgccttc
    38581 aataataaat ctgtcggcca acgagcggcg attattgccg caggtccggt tgcaaacttc
    38641 atttttgcta tctttgccta ctggctggtt tttattattg gtgtgcctgg cgtacgtccg
    38701 gtggttggcg aaatagcagc caattcgata gctgcggaag cacaaattgc accaggtacg
    38761 gaactaaaag ccgtagatgg tatcgaaacg cctgattggg atgccgtgcg tttgcagttg
    38821 gtcgataaaa ttggcgatga aagcaccacc attacagtag cgccatttgg cagcgaccaa
    38881 cggcgggatg taaagctcga tttacgtcac tgggcgtttg agcctgataa agaagatccg
    38941 gtatcttcgc tggggattcg tcctcgtggg ccgcaaattg aacctgtact ggaaaatgtg
    39001 cagccaaact cggcggcaag caaggcaggt ttgcaagcag gcgacaggat cgttaaagtc
    39061 gatggtcagc ccttaacgca gtgggtgacc tttgtgatgc ttgtccggga taacccgggt
    39121 aaatccttag cgttagaaat cgaaaggcag gggagtccct tgtctttgac attaatcccg
    39181 gagagtaaac cgggtaatgg taaagcgatt ggttttgtcg gtattgagcc gaaagtcatt
    39241 cctttgccag atgagtataa agttgtacgc cagtatgggc cgttcaacgc catcgtcgaa
    39301 gccacggaca aaacgtggca gctgatgaag ctgacggtca gtatgctggg aaaattgatc
    39361 accggtgatg tgaaactgaa caacctcagt gggccgatct ctatcgccaa gggggctggg
    39421 atgacagcgg aactcggggt agtttattac ctgccgtttc ttgcgcttat tagcgtgaac
    39481 ttagggataa ttaacctgtt tccgttgccc gtacttgacg gggggcatct gctgttcctt
    39541 gcgatcgaaa agatcaaagg cggaccggta tccgagcggg ttcaagactt ttgttatcgc
    39601 attggctcga ttctgctggt gctgttaatg gggcttgcac ttttcaatga tttctctcgg
    39661 ttatgagagt tagttaggaa gaacgcataa taacgatggc gatgaaaaag ttgctcatag
    39721 cgtcgctgct gtttagcagc gccaccgtat acggtgctga agggttcgta gtgaaagata
    39781 ttcatttcga aggccttcag cgtgtcgccg ttggtgcggc cctcctcagt atgccggtgc
    39841 gcacaggcga cacggttaat gatgaagata tcagtaatac cattcgcgct ctgtttgcta
    39901 ccggcaactt tgaggatgtt cgcgtccttc gtgatggtga tacccttctg gttcaggtaa
    39961 aagaacgtcc gaccattgcc agcattactt tctccggtaa caaatcggtg aaagatgaca
    40021 tgctgaagca aaacctcgag gcttctggtg tgcgtgtggg cgaatccctc gatcgcacca
    40081 ccattgccga tatcgagaaa ggtctggaag acttctacta cagcgtcggt aaatatagcg
    40141 ccagcgttaa agctgtcgtg accccgctgc cgcgcaaccg tgttgaccta aaactggtgt
    40201 tccaggaagg tgtgtcagct gaaatccagc aaattaacat tgttggtaac catgctttca
    40261 ccaccgacga actgatctct catttccaac tgcgtgacga agtgccgtgg tggaacgtgg
    40321 taggcgatcg taaataccag aaacagaaac tggcgggcga ccttgaaacc ctgcgcagct
    40381 actatctgga ccgcggttat gcccgtttca acatagactc tacccaggtc agtctgacgc
    40441 cagataaaaa aggtatttac gtcacggtga acatcaccga aggcgatcag tacaagcttt
    40501 ctggcgttga agtgagcggc aaccttgccg ggcactccgc tgaaattgag cagctgacta
    40561 agatcgagcc gggcgagctg tataacggca ccaaagtgac caaaatggaa gatgacatta
    40621 aaaagcttct cggtcgctat ggttatgcct atccgcgcgt acagtcgatg cctgaaatta
    40681 acgatgccga caaaaccgtt aaattacgcg tgaacgttga tgcgggtaac cgtttctacg
    40741 tgcgtaagat ccgttttgaa ggtaacgata cctcgaaaga tgccgtcctg cgtcgcgaaa
    40801 tgcgtcagat ggaaggtgca tggctgggaa gcgatctggt cgatcagggt aaggagcgtc
    40861 tgaatcgtct gggcttcttt gaaactgtcg ataccgatac ccaacgtgtt ccgggtagcc
    40921 cggaccaggt agatgtagtc tacaaggtga aagaacgtaa cactggtagc ttcaacttcg
    40981 ggattggtta cggtactgaa agtggcgtga gcttccaggc tggcgtgcaa caggataact
    41041 ggttaggtac aggttatgct gttggtatca acgggaccaa aaacgattac cagacctatg
    41101 ctgaactgtc ggtaaccaac ccgtacttca ccgtagatgg cgtaagcctc ggtggtcgtc
    41161 tcttctataa tgacttccag gcagatgacg ccgacctgtc cgactatacc aacaagagtt
    41221 atggtacaga cgtgacgttg ggcttcccga ttaacgaata taactcgctg cgtgcaggtc
    41281 tgggttatgt acataactcc ctgtccaaca tgcagcctca ggttgcgatg tggcgttatc
    41341 tgtactctat gggtgaacat ccgagcacct ctgatcagga taacagcttc aaaacggacg
    41401 acttcacgtt caactatggt tggacctata acaagcttga ccgtggttac ttcccgacag
    41461 atggttcacg tgtcaacctg accggtaaag tgaccattcc tggatcggat aacgaatact
    41521 acaaagtgac gttagacacg gcgacttatg tgccgatcga tgacgatcac aaatgggttg
    41581 ttctggggcg tacccgctgg ggttatggtg atggtttagg cggcaaagag atgccgttct
    41641 acgagaactt ctatgccggt ggttccagca ccgtgcgtgg cttccagtcc aatacaattg
    41701 gtccgaaagc agtttacttc ccgcatcagg ccagtaatta tgatccggac tatgattacg
    41761 aatgtgcgac tcaggacggc gcgaaagacc tgtgtaaatc ggatgatgct gtaggcggta
    41821 acgccatggc ggttgccagc ctcgagttca tcaccccgac gccgtttatt agcgataagt
    41881 atgctaactc ggttcgtact tccttcttct gggatatggg taccgtttgg gatacaaact
    41941 gggattccag ccaatattct ggatatccgg actatagtga tccaagcaat atccgtatgt
    42001 ctgcgggtat cgcattacaa tggatgtccc cattggggcc gttggtgttc tcctacgccc
    42061 agccgttcaa aaagtacgat ggagacaagg cagaacagtt ccagtttaac atcggtaaaa
    42121 cctggtaagt gttctccaca aaggaatgta gtggtagtgt agcgatgact ttaggcgatc
    42181 aatataaatc gccgggccac gcaaagaact gcaccctccg gtgcaaatgg gatggtaagg
    42241 agtttattgt gaaaaagtgg ttattagctg caggtctcgg tttagcactg gcaacatctg
    42301 ctcaggcggc tgacaaaatt gcaatcgtca acatgggcag cctgttccag caggtagcgc
    42361 agaaaaccgg tgtttctaac acgctggaaa atgagttcaa aggccgtgcc agcgaactgc
    42421 agcgtatgga aaccgatctg caggctaaaa tgaaaaagct gcagtccatg aaagcgggca
    42481 gcgatcgcac taagctggaa aaagacgtga tggctcagcg ccagactttt gctcagaaag
    42541 cgcaggcttt tgagcaggat cgcgcacgtc gttccaatga agaacgcggc aaactggtta
    42601 ctcgtatcca gactgctgtg aaatccgttg ccaacagcca ggatatcgat ctggttgttg
    42661 atgcaaacgc cgttgcttac aacagcagcg atgtaaaaga catcactgcc gacgtactga
    42721 aacaggttaa ataagtaatg ccttcaattc gactggctga tttagcgcag cagttggatg
    42781 cagaactaca cggtgatggc gatatcgtca tcaccggcgt tgcgtccatg caatctgcac
    42841 aaacaggtca cattacgttc atggttaacc caaaataccg tgagcattta ggcttgtgcc
    42901 aggcgtccgc ggttgtcatg acccaggacg atcttccttt cgcgaaaagt gccgcgctgg
    42961 tagtgaagaa tccctacctg acttacgcgc gcatggcgca aattttagat accacgccgc
    43021 agcccgcgca gaacattgca cccagtgcgg tgatcgacgc gacggcgaag ctgggtaaca
    43081 acgtatcgat tggcgctaac gcggtgattg aatccggcgt tgaactgggc gataacgtga
    43141 ttatcggtgc cggttgcttc gtaggtaaaa acagcaaaat cggtgcaggt tcgcgtctct
    43201 gggcgaacgt aaccatttac catgagatcc agatcggtca gaattgcctg atccagtccg
    43261 gaacagtggt aggcgcagac ggctttggtt atgccaacga tcgtggtaac tgggtgaaga
    43321 tcccacagat tggtcgcgta attattggcg atcgcgtgga gatcggtgcc tgcactacca
    43381 tcgatcgcgg cgcgctggat gacactgtta ttggcaatgg cgtgatcatt gataaccagt
    43441 gccagattgc acataacgtc gtgattggcg acaatacggc ggttgccggt ggcgtcatta
    43501 tggcgggcag cctgaaaatt ggtcgttact gcatgatcgg cggagccagc gtaatcaacg
    43561 ggcatatgga aatatgcgac aaagtgacgg ttacgggcat gggtatggtg atgcgtccca
    43621 tcactgaacc aggcgtctat tcctcaggca ttccgctgca acccaacaaa gtctggcgca
    43681 aaaccgctgc actggtgatg aacattgatg acatgagcaa gcgtctgaaa tcgcttgagc
    43741 gcaaggttaa tcaacaagac taacgttcca tcttttgttc gccaaacttt acggcctgtc
    43801 tcattcttac gattgcggca ggccgtgtta ttattgtcgt ttcttatatt ttgacaggaa
    43861 gagtatcttg actactaaca ctcatactct gcagattgaa gagattttag aacttctgcc
    43921 gcaccgtttc ccgttcttac tggtggatcg cgtgctggat tttgaagaag gtcgttttct
    43981 gcgcgcagta aaaaatgtct ctgtcaatga gccattcttc cagggccatt tccctggaaa
    44041 accgattttc ccgggtgtgc tgattctgga agcaatggca caggcaacag gtattctggc
    44101 gtttaaaagc gtaggaaaac tggaaccggg tgagctgtac tacttcgctg gtattgacga
    44161 agcgcgcttc aagcgcccgg tcgtgcctgg cgatcaaatg atcatggaag tcactttcga
    44221 aaaaacgcgc cgcggcctga cccgttttaa aggggttgct ctggtcgacg gtaaagtagt
    44281 ttgcgaagca acgatgatgt gtgctcgtag ccgggaggcc tgatacgtga ttgataaatc
    44341 cgcctttgtg catccaaccg ccattgtgga agagggcgcg tcgattggcg cgaacgcaca
    44401 cattggtcct ttttgtatcg ttggacccca tgtcgaaatt ggtgagggta ccgtactgaa
    44461 atctcacgtt gtcgtgaatg gtcatactaa aattggccgc gataatgaga tttatcagtt
    44521 cgcctccatc ggcgaagtta accaggatct gaaatatgct ggcgaaccga cccgcgtgga
    44581 aatcggcgat cgtaaccgca ttcgcgaaag cgtcaccatt catcgtggca cagtccaggg
    44641 cggtggattg acgaaggtgg gcagcgacaa cttactgatg atcaacgcgc acattgcgca
    44701 cgattgtacg gtaggtaacc gctgtattct cgccaacaac gcaacgctgg cgggtcacgt
    44761 atcggttgac gacttcgcga tcatcggcgg catggccgca gtccatcagt tctgcatcat
    44821 tggtgcgcat gtgatggttg gcggctgctc cggtgtggcg caggacgtcc ctccttatgt
    44881 cattgcgcag ggtaaccacg ccacgccgtt cggtgtcaat atcgaagggc tgaagcgccg
    44941 cggattcagc cgtgaggcga ttaccgctat ccgcaatgcg tataagctga tttatcgtag
    45001 cggtaaaacg ctcgatgaag tgaaaccgga aattgctgaa ctggcggaaa catatccgga
    45061 agtgaaagcc tttaccgatt tctttgcacg ctcaacgcgc ggtctgattc gttaatgact
    45121 gaacagcgtc cattaacgat tgccctggtc gccggagaaa cctccggcga tatcctgggg
    45181 gccggtttaa tccgcgctct gaaagaacgt gtgcccaacg cccgctttgt tggtgttgcc
    45241 gggccacgaa tgcaggctga aggctgcgaa gcctggtacg aaatggaaga gttggcggtg
    45301 atgggcattg ttgaagtgct cggtcgtctg cgtcgcttac tgcatattcg tgccgatctg
    45361 acaaagcgtt ttggtgaact aaagccagat gtttttgttg gcattgatgc gcctgacttc
    45421 aatattatcc tcgaaggtaa ccttaaaaag cagggtatca aaaccattca ttatgtcagt
    45481 ccgtccgtct gggcgtggcg acagaaacgc gttttcaaaa taggcagagc caccgatctg
    45541 gtgctcgcat ttctgccttt cgaaaaagcg ttttatgaca aatacaacgt accgtgccgc
    45601 tttatcggtc ataccatggc tgatgccatg ccattagatc cagataaaaa tagtgcccgt
    45661 gatgtgctgg ggatccctta cgatgcccac tgtctggcat tgttgccggg cagccgtggt
    45721 gcagaagtcg aaatgcttag tgccgatttc ctgaaaacgg cccagctttt gcgccagaca
    45781 tatccggatc tcgaaatcgt ggtgccactg gtgaatgcca aacgccgcga gcagtttgaa
    45841 cgcatcaaag ctgcagtcgc gccagacctg tcggttcatt tgctggatgg aatgggccgt
    45901 gaggcgatgg tcgccagcga tgcggcacta ctggcgtcgg ggacggcagc cctggagtgt
    45961 atgctggcga aatgcccgat ggtggtggga tatcgcatga agccttttac cttctggttg
    46021 gcgaagcggc tggtgaaaac tgattatgtc tcgctgccaa atctgctggc gggcagagag
    46081 ttagtcaaag agttattgca ggaagagtgt gagccgcaaa aactggctgc ggcgctgtta
    46141 ccgctgttgg cgaatgggaa aaccagccac gcgatgcacg ataccttccg tgaactgcat
    46201 cagcagatcc gctgcaatgc cgatgagcag gcggcacaag ccgttctgga gttagcacaa
    46261 tgatcgaatt tgtctatccg cacacgcagc tggttgcggg tgtggatgaa gtcggacgcg
    46321 ggccgttagt tggcgcggtc gtcaccgctg cggtgattct cgacccggcg cgcccgattg
    46381 ccgggctgaa tgattccaaa aagctgagcg aaaaacgtcg tctggcgctc tgtgaagaga
    46441 tcaaagagaa agcgttgagc tggagtctgg gccgcgcgga acctcacgaa atcgacgaac
    46501 tgaatatttt acatgcgacc atgctggcga tgcagcgagc cgtcgctggg ctgcatattg
    46561 cgccggaata tgtgttgatt gatggtaacc gctgcccgaa attaccgatg cctgcgatgg
    46621 ctgtggtgaa aggcgatagc cgcgtaccgg aaatcagtgc cgcgtctatc ctggcgaaag
    46681 tgacgcgtga cgccgaaatg gcggcgctgg atattgtttt cccgcaatat ggttttgccc
    46741 aacacaaagg gtatccaacc gcttttcatc tggaaaaact ggctgaacac ggcgcgaccg
    46801 aacaccatcg gcgcagcttt gggcctgtca aacgcgcact gggacttgcg tcctgattct
    46861 tgtgtcgaga ttaagtaaac cggaatctga agatgtctga accacgtttc gtacacctgc
    46921 gggtgcacag cgactactcg atgatcgatg gcctggccaa aaccgcaccg ctggtaaaaa
    46981 aggcggcggc gttgggtatg ccagcactgg cgatcaccga tttcaccaac ctttgcggtc
    47041 tggtgaagtt ctacggagcg ggacatggcg cagggattaa gcctatcgtc ggggcagatt
    47101 ttaacgtcca gtgcgacctg ctgggtgatg agttaaccca cctgacggta ctggcggcga
    47161 acaataccgg ctatcagaat ctgacgttgc tgatctcaaa agcgtatcag cgcgggtacg
    47221 gtgccgccgg gccgatcatc gatcgcgact ggcttatcga attaaacgaa gggttgatcc
    47281 ttctttccgg cgggcgcatg ggcgacgtcg gacgcagtct tttgcgtggt aacagcgcgc
    47341 tggtagatga gtgtgtcgcg ttttatgaag aacacttccc ggatcgctat tttctcgagc
    47401 tgatccgcac cggcaggccg gatgaagaaa gctatctgca cgcggcggtg gaactggcgg
    47461 aagcgcgcgg tttgcccgtc gtggcgacta acgatgtgcg ctttatcgac agcagcgact
    47521 ttgacgcaca cgaaatccgc gtcgcgatcc acgacggctt taccctcgac gatcctaaac
    47581 gcccgcgtaa ctattcgccg cagcaatata tgcgtagcga agaggagatg tgcgagctgt
    47641 ttgccgacat cccagaagcc cttgccaaca ccgttgagat cgccaaacgc tgtaacgtaa
    47701 ctgtgcgtct tggtgaatac ttcctgccgc agttcccgac cggggacatg agcaccgaag
    47761 attatctggt caagcgtgca aaagagggcc tggaagagcg tctggccttt ttattccctg
    47821 atgaggaaga acgtgttaag cgccgcccgg aatatgacga acgtctggag actgaacttc
    47881 aggttatcaa ccagatgggc ttcccgggct acttcctcat cgttatggaa tttatccagt
    47941 ggtcgaaaga taacggcgta ccggtagggc caggccgtgg ctccggtgcg ggttcactgg
    48001 tggcctacgc gctgaaaatc accgacctcg atccgctgga atttgacctg ctgttcgaac
    48061 gtttccttaa cccggaacgt gtctccatgc ctgacttcga cgttgacttc tgtatggaga
    48121 aacgcgatca ggttatcgaa cacgtagcgg acatgtacgg tcgtgatgcg gtatcgcaga
    48181 ttatcacctt cggtacgatg gcggcgaaag cggtgatccg cgacgtaggc cgcgtgctgg
    48241 ggcatccgta cggctttgtc gatcgtatct cgaaactgat cccgcccgat ccggggatga
    48301 cgctggcgaa agcgtttgaa gccgagccgc agctgccgga aatctacgaa gcggatgaag
    48361 aagttaaggc gttgatcgac atggcgcgca aactggaagg ggtcacccgt aacgccggta
    48421 agcacgccgg tggggtggtt atcgcgccga ccaaaattac cgattttgcg ccgctttatt
    48481 gcgatgaaga gggcaaacat ccggtcaccc agtttgataa aagcgacgtt gaatacgccg
    48541 gactggtgaa gttcgacttc cttggtttgc gtacgctcac catcatcaac tgggcgctgg
    48601 agatgatcaa caagcggcgg gcgaagaatg gcgagccgcc gctggatatc gccgcgatcc
    48661 cgctggatga caagaaaagc ttcgacatgc tgcaacgctc ggaaaccacg gcggtattcc
    48721 agcttgaatc gcgcggcatg aaggacctga tcaagcgtct gcaacctgac tgcttcgaag
    48781 atatgatcgc actggtggca ctgttccgcc ctggtccgtt gcaatcaggg atggtggata
    48841 actttatcga ccgtaaacat ggtcgcgaag agatctccta tccggacgtg cagtggcagc
    48901 atgaaagcct gaaaccggta ctggagccaa cctacggcat tatcctgtat caggaacagg
    48961 tcatgcagat tgcgcaggtg ctttctggtt ataccctcgg tggcgcggat atgctgcgtc
    49021 gtgcgatggg taagaaaaag ccggaagaga tggctaagca acgttctgta tttgctgaag
    49081 gtgcagaaaa gaacggaatc aacgccgaac tggcgatgaa aatcttcgac ctggtggaga
    49141 aattcgctgg ttacggattt aacaaatcgc actctgcggc ctatgctttg gtgtcatatc
    49201 aaacattatg gttgaaagcg cactatccgg cagagtttat ggcggcggta atgaccgccg
    49261 atatggacaa caccgagaag gtggtgggcc tggtggatga gtgctggcgg atggggctga
    49321 aaatcctgcc accagatata aactccggtc tttaccattt ccacgtcaac gacgacggcg
    49381 aaatcgtgta tggtattggc gcgatcaaag gggtaggtga aggtccgatt gaggcgatca
    49441 tcgaagcccg taataaaggc ggctacttcc gcgaactgtt tgatctctgc gcccgaaccg
    49501 acaccaaaaa gttgaaccgt cgcgtgctgg aaaaactgat catgtccggg gcgtttgacc
    49561 gtcttgggcc acatcgcgcg gcgctgatga actcgctggg cgatgcgtta aaagcggcag
    49621 atcaacacgc gaaagcggaa gctatcggtc aggccgatat gttcggcgtg ctggccgaag
    49681 agccggaaca aattgaacaa tcctatgcca gctgccaacc gtggccggag caggtggtat
    49741 tagatgggga acgtgaaacg ttaggcctgt acctgaccgg acaccctatc aaccagtatt
    49801 taaaagagat tgagcgttat gtcggaggcg taaggctgaa agacatgcac ccgacagaac
    49861 gtggtaaagt catcacggct gcggggctcg ttgttgccgc gcgggttatg gtcaccaagc
    49921 gcggcaatcg tatcggtatc tgcacgctgg atgaccgttc cgggcggctg gaagtgatgt
    49981 tgtttactga cgccctggat aaataccagc atttgctgga aaaagaccgc atacttatcg
    50041 tcagcggaca ggtcagcttt gatgacttca gcggtgggct taaaatgacc gctcgcgaag
    50101 tgatggatat tgacgaagcc cgggaaaaat atgctcgcgg gcttgctatc tcgctgacgg
    50161 acaggcaaat tgatgaccag cttttaaacc gactccgtca gtctctggaa ccccaccgct
    50221 ctgggacaat tccagtacat ctctactatc agagggcgga tgcacgcgcg cggttgcgtt
    50281 ttggcgcgac gtggcgtgtc tctccgagcg atcgtttatt aaacgatctc cgtggcctca
    50341 ttggttcgga gcaggtggaa ctggagtttg actaatacag gaatactatg agtctgaatt
    50401 tccttgattt tgaacagccg attgcagagc tggaagcgaa aatcgattct ctgactgcgg
    50461 ttagccgtca ggatgagaaa ctggatatta acatcgatga agaagtgcat cgtctgcgtg
    50521 aaaaaagcgt agaactgacg cgtaaaatct tcgccgatct cggtgcatgg cagattgcgc
    50581 aactggcacg ccatccacag cgtccttata ccctggatta cgttcgcctg gcatttgatg
    50641 aatttgacga actggctggc gaccgcgcgt atgcagacga taaagctatc gtcggtggta
    50701 tcgcccgtct cgatggtcgt ccggtgatga tcattggtca tcaaaaaggt cgtgaaacca
    50761 aagaaaaaat tcgccgtaac tttggtatgc cagcgccaga aggttaccgc aaagcactgc
    50821 gtctgatgca aatggctgaa cgctttaaga tgcctatcat cacctttatc gacaccccgg
    50881 gggcttatcc gggcgtgggc gcagaagaac gtggtcagtc tgaagccatt gcacgcaacc
    50941 tgcgtgaaat gtctcgcctc ggcgtaccgg tagtttgtac ggttatcggt gaaggtggtt
    51001 ctggcggtgc gctggcgatt ggcgtgggcg ataaagtgaa tatgctgcaa tacagcacct
    51061 attccgttat ctcgccggaa ggttgtgcgt ccattctgtg gaagagcgcc gacaaagcgc
    51121 cgctggcggc tgaagcgatg ggtatcatcg ctccgcgtct gaaagagctg aaactgatcg
    51181 actccatcat cccggaaccg ctgggcggcg ctcaccgtaa cccggaagcg atggcggcat
    51241 cgttgaaagc gcaactgctg gcggatctgg ccgatctcga cgtgttaagc actgaagatt
    51301 taaaaaatcg tcgttatcag cgcctgatga gctacggtta cgcgtaattc gcaaaagttc
    51361 tgaaaaaggg tcacttcggt ggcccttttt tatcgccacg gtttgagcag gctatgatta
    51421 aggaaggatt ttccaggagg aacacatgaa catcattgcc attatgggac cgcatggcgt
    51481 cttttataaa gatgagccca tcaaagaact ggagtcggcg ctggtggcgc aaggctttca
    51541 gattatctgg ccacaaaaca gcgttgattt gctgaaattt atcgaacata accctcgaat
    51601 ttgcggcgtg atttttgact gggatgaata cagtctcgat ttatgtagcg atatcaatca
    51661 gcttaatgaa tatctcccgc tttatgcctt catcaacacc cactcgacga tggatgtcag
    51721 cgtgcaggat atgcggatgg cgctctgttt ttttgaatat gcgctggggc aggcggaaga
    51781 tatcgccatt cgtatgcgtc agtacaccga cgaatatctt gataacatta ccccaccgtt
    51841 cacgaaagcc ttgtttacct acgtcaaaga gcggaagtac accttttgta cgccgggtca
    51901 tatgggcggc accgcatatc aaaaaagccc ggttggctgt ctgttttatg actttttcgg
    51961 cgggaatacc cttaaggctg atgtctctat ttcggtcacc gagcttggtt cgttgctcga
    52021 ccacaccggg ccacacctgg aagcggaaga gtacatcgcg cggactttcg gcgcggaaca
    52081 gagttatatc gttaccaacg gaacatcgac gtcgaacaaa attgtgggta tgtacgccgc
    52141 gccatccggc agtacgctgt tgattgaccg caattgtcat aaatcgctgg cgcatctgtt
    52201 gatgatgaac gatgtagtgc cagtctggct gaaaccgacg cgtaatgcgt tggggattct
    52261 gggtgggatc ccgcgccgtg aatttactcg cgacagcatc gaagagaaag tcgctgccac
    52321 cacgcaggca caatggccgg ttcatgcggt gattaccaac tcaacctatg atggcttgct
    52381 ctacaacacc gactggatca aacagacgct ggatgtcccg tcgatccact tcgattccgc
    52441 ctgggtgccg tacacccatt ttcatccgat ctaccagggg aaaagtggta tgagcggcga
    52501 gcgtgttgcg ggaaaagtga tcttcgaaac gcaatcgacc cacaaaatgc tggcggcgtt
    52561 atcgcaggct tcgctgatcc acattaaagg tgagtatgac gaagaggcgt ttaacgaagc
    52621 ctttatgatg cataccacca cctcgcccag ttatcccatt gttgcttcgg ttgagacggc
    52681 agcagcgatg ctgcgtggta atccgggcaa acggctgatt aaccgttcag tagaacgagc
    52741 tctgcatttt cgcaaagagg tccagcggct gcgggaagag tctgacggtt ggtttttcga
    52801 tatctggcaa ccgccgcagg tggatgaagc cgaatgctgg cccgttgcgc ctggcgaaca
    52861 atggcacggc tttaacgatg cggatgccga tcatatgttc ctcgatccgg ttaaagtcac
    52921 tattctgacg ccggggatgg acgagcaggg caatatgagc gaggagggga tcccggcggc
    52981 gctggtggcg aagttcctcg atgagcgtgg gatcgtggta gagaaaaccg gcccatataa
    53041 cctgctgttt ctctttagta ttggcatcga taaaaccaaa gcaatgggat tattgcgtgg
    53101 gttgacggaa tttaaacgct cttacgatct caatctgcga atcaaaaata tgcttccgga
    53161 tctgtacgca gaagatcccg atttctaccg caatatgcgt attcaggatc tggcgcaagg
    53221 gatccataag ctgattcgta aacacgatct tcccggtttg atgttgcggg cattcgatac
    53281 tttgccggag atgatcatga cgccacatca ggcatggcag agacagatta aaggtgaagt
    53341 agaaaccatt gcgctggaac aactggtcgg tagagtatcg gcaaatatga tcctgcctta
    53401 tccaccgggc gtaccgctgt tgatgcctgg agaaatgctg accaaagaga gccgcacagt
    53461 actcgatttt ctactgatgc tttgttccgt agggcaacat taccccggtt ttgaaacgga
    53521 tattcacggt gcgaaacagg acgaagacgg cgtttaccgc gtacgagtcc taaaaatggc
    53581 gggataactt gccagagcgg cttccgggcg agtaacgtgc tgttaacaaa taaaggagac
    53641 gttatgctgg gtttaaaaca ggttcaccat attgcgatta ttgcgacgga ttatgcggtg
    53701 agcaaagctt tctactgcga tattcttggt ttcacgctgc aaagcgaagt ctatcgcgaa
    53761 gcgcgcgact cgtggaaagg ggatttggcg cttaatgggc aatatgtgat tgagcttttc
    53821 tcatttccgt tcccgccgga gcgccccagc cgaccggaag cttgcggcct gcgtcatctg
    53881 gcttttagcg ttgatgacat cgatgcggca gtggcgcacc ttgaaagcca taacgtgaag
    53941 tgtgaagcca tccgtgtcga tccatacacg caaaaacgct tcaccttctt taacgatccg
    54001 gacgggctgc cgttggaact gtatgagcag taaggcttgt catcgccgca tttgcccggt
    54061 aacgtgccgg gcattgctac tgtaaaatcg caccatcatg acactcacgc tcaatagaca
    54121 acttctcacc tcacgccaga ttctggtggc ctttagcggc gggcttgact ccaccgttct
    54181 gctgcatcag ttggtgcagt ggcggacgga aaatccgggg ggcgctctgc gcgctattca
    54241 tgtgcatcac ggtttaagtg ccaatgccga tgcctgggtt acgcattgcg aaaacgtctg
    54301 ccaacagtgg caggtgccgc tggtggtcga acgcgtacaa cttgcgcaag aggggctggg
    54361 gattgaggcc caggcgcggc aggcgcgtta tcaggcattt gcccgcacct tgttgcccgg
    54421 tgaagtgctg gtcaccgcgc aacatctcga cgatcaatgt gaaacttttc tgctggcgct
    54481 aaaacgcggt agcggccctg ccgggctttc ggctatggcg gaagtttcgg agtttgccgg
    54541 aacacggctt atccgcccgt tgcttgcccg cacgcggggg gaactggcgc agtgggcatt
    54601 ggcgcatggt ttacgctgga ttgaagacga aagtaatcag gacgactcat acgatcgtaa
    54661 ctttctgcgc ctgcgcgtag tgccgttatt gcagcagcgt tggccgcatt ttgccgaaac
    54721 aacggcccgc agcgccgcac tttgtgctga acaagagagc ctgctggatg aactgctggc
    54781 agatgattta gcacactgtc aatcgccgca ggggacgctg cagattgtgc caatgctggc
    54841 gatgagtgat gcccgccgcg cggcgattat ccgccgctgg ctggcagggc agaatgcacc
    54901 gatgccttcc cgcgacgcgt tggtgaggat ctggcaggaa gtggcgctgg cgcgggaaga
    54961 tgcctccccc tgtttacgtt tgggcgcgtt tgaaatccga cgctatcagt cgcaactgtg
    55021 gtggattaaa tccgtcaccg ggcaaagcga aaacattgtg ccgtggcaga catggcttca
    55081 accgctggaa ttaccggcgg ggcagggaag tgtacagctt aatgcgggag gcgatattcg
    55141 ccctccgcgt gcagacgaag cggtcagcgt gcgtttcaaa gcgccaggat tgctgcatat
    55201 tgtcgggcgt aacggcggac gtaagctaaa gaaaatctgg caagagctgg gcgtgccgcc
    55261 gtggctacgt gacaccacgc cactgctgtt ttatggcgaa acgctgattg cggcggcagg
    55321 gggatttgtg acgcaagaag gtgtggctga aggtgagaat ggcataagtt ttgtctggca
    55381 gcgaaacgct tagtttagtg aaggccggat atgacgcatt aagcgtcgca tccggcgaaa
    55441 gtcaatcagg actcgctcac caccaccgta ccgatttccg ggtggctaaa gctggtaatt
    55501 ttatccagac gtagctcacg ggtttcgcca gcggcctcga cgaccagata ctccacattt
    55561 ttgcgggaga ctaaatcgct ggctttggcc tgcaattttt cgccatcttt cagctcaagt
    55621 gtcagcatta aatgatgctg gcaggcgagc tcaagattat cgtaatcatc acaattgatt
    55681 ggttgatacg tatcattcat tgacataatc gctcaccagt aagtttgccg cagcgtatgc
    55741 tgctttttcc ctgacagcct cagaaagggc gtcgtcggca gccatttcat tcagcacttt
    55801 caaaacgcag cccagcgcgt ccggaacgta tcctaagtct ccgctggcga tttccgcgta
    55861 ccgcttgcgt attaactcac aatatttttc cacatgccct cctgtcagca ctctgactta
    55921 accgtggatg caagtctaag cctacgaaga taaactctgt ttcgcaaggt gactatacca
    55981 cactcatttc tgcaatatca gcgccgcaac tgcacgtatt ccgttacaat ggcctcctga
    56041 ttcgaaagga gttttcttat ggcgcttaaa gcgacaattt ataaagcgac ggttaatgtg
    56101 gccgatctcg accgcaacca gtttctcgat gcctctctga cgctggcgcg ccacccttca
    56161 gaaacccagg agcgtatgat gctgcgcctg ctggcgtggc tgaaatatgc tgatgaacgt
    56221 ctgcaattta cccgtggttt gtgtgccgat gatgagccgg aagcgtggct gcgtaacgat
    56281 cacctgggca ttgatttgtg gattgagctg gggctgccgg atgagcgacg aattaagaaa
    56341 gcctgcaccc aggccgcaga agtggcgctg tttacctata atagtcgggc ggcgcaaatc
    56401 tggtggcagc aaaatcagag caaatgtgcg cagtttgcca atctttccgt ctggtatctg
    56461 gacgacgagc agctggcgaa agtgagcgcc tttgccgatc gtaccatgac gctgcaggca
    56521 acgattcagg atggcgtgat ctggttatcg gatgataaga ataatctgga agtgaactta
    56581 accgcctggc aacaaccttc atgattgtga tttcccgaca tgttgctatt cccgatggtg
    56641 agcttgagat caccgccatt cgtgcgcagg gcgcgggcgg gcagcatgtt aataagacct
    56701 caacggccat tcatctgcgt tttgacattc gggcatccag cctgccagag tattacaaag
    56761 agcgtctgct cgccgccagc catcatttga tcagcagtga tggcgtaatt gtcattaagg
    56821 cacaggaata ccgcagtcag gaactgaacc gcgaagcggc gctggcccgg ctggtggcag
    56881 tgattaaaga tttaacaaca gaacaaaaag cccgacgacc cacgcggccc acccgtgcat
    56941 cgaaagagcg caggctggca tcgaaagcac aaaaatcaag cgtgaaggcg atgcgcagcg
    57001 gtcgggaata aaaagaagga atggatggtg aaaaaagcga tagtgacagc gatggctgta
    57061 atcagcctct ttactctgat gggttgtaat aatcgggccg aagtcgatac gctttctccg
    57121 gcgcaggctg ccgaattgaa accgatgccg caaagttggc gcggcgtgct gccgtgtgcc
    57181 gattgcgaag gaatcgaaac ctctctgttc ctcgaaaaag acggaacatg ggtgatgaat
    57241 gagcgttatc tcggtgctcg tgaagaacct tcctccttcg cttcctacgg tacatgggcg
    57301 cgaaccgctg acaagctggt attaaccgac agcaaaggtg aaaagtcata ttatcgggcg
    57361 aaaggcgatg cgctggagat gctcgatcgt gaaggcaatc cgattgaatc gcagttcaac
    57421 tatacgctgg aaccggcaca atccagttta cccatgacgc cgatgaccct gcggggcatg
    57481 tatttttata tggctgatgc ggcgaccttc actgattgcg cgaccggaaa acgtttcatg
    57541 gtagcgaata acgcagagct ggagcgtggc tacctggctg cgcgcggcca cagtgaaaaa
    57601 ccggtgttac tgtcagtaga aggtcacttt acgcttgagg ctaatccgga taccggtgcg
    57661 ccaaccaaag tattagcacc cgatacggca gggaaatttt acccaaacca ggattgcagt
    57721 agtttggggc agtaacccgt cttgagacag aaacaaacgc aaaacaggcc agaaggatat
    57781 atttcaacat tttgaatttg cacgtttttt gtaggccgga taaggcgttt acgccgcatc
    57841 cggcaatggt gctcaacgcc tgatgcgacg ctggcgcgtc ttatcatgcc tacaaccccc
    57901 ctcatacctt aaggcggata aggcatttac tttatcaccg ggtcaaacca acaaagcgac
    57961 ccgccttgat ataaatccct ggcttcaggt gcccaacata ctgtaactgc gtttctggct
    58021 taaacgcaga gacatcacca atacgcatat gcatcagatc ggcagggcta atccagcccg
    58081 attgcgccag cgtaagcgga tgcccggctt tggcaaatgc atcagtgaca aattccgaac
    58141 aaaaccacga ctttttgtct ccttcgacca cactgcttaa ctgcgctttc gccaggccgc
    58201 tgacgcactg ttggcgaaaa tcctcggaga acggattcag tgagcacatc tggcgagtca
    58261 ccataaaggg aataaattcg acaatgccgc gatagttata accgctatct ttgattttat
    58321 tggcgaacgc ggtgatttct gtggcttgtt gcggggtaag atccgggact cgtaagacga
    58381 aaagtttatc actatgcttc atcgcttttt taagggaaac aatctggacg ccagcgcctg
    58441 tcgcttctgc aacgttattt tcacccagat agattgcaac gtgactcaca gaggaagtgc
    58501 tgaaaacgcg gatcccaaat gaggttacct ccaggcttga ggagaacagc aaatcgccgg
    58561 gtttgagatc aggtaccgtt atttctttta ttgattgttc ggtgaaagag ctttgatgct
    58621 ggaatttgac agcccatgtt ttcgcctcgg catctactgc tgtggctgat gagtctggct
    58681 ggctgatatc aacggtacag gccgaaagta aaagaaagca ggggagaagc aggcggcagt
    58741 acgcctttgg tttatccatt ttatacaatc catgtaaaaa agggccctga aattcaggac
    58801 cctttctggc atcagccttt aatctgtttc accagatatt cgacgatgtc accagtctta
    58861 attaactgtt tctcgccgtt gcgacgatat ttatattcga tatcgtcgtt gtcgaggtta
    58921 cggtcgccca gcacaatagt gtgcggaata ccgatcagtt ccatatcggc aaacatcacg
    58981 cccggacgtt cttttcggtc atccagcagc acttcgatac cttgtgcacg cagttcgctg
    59041 tacagtttct cagcaagctc ttgtacgcgg aaggatttgt gcatgttcat cggcagaatt
    59101 gccacctgga acggcgcgat agcgtcaggc catacgatgc cgcgctcgtc gtagttctgc
    59161 tcaatcgccg cagccactac gcgcgttacc ccgataccgt agcaacccat agtcaggatt
    59221 tggttacggc catcttcacc ctgtacggag gctttcagtg cttcggagta cttggtaccc
    59281 agctggaaga tgtgaccaac ttcgatacca cgtttgatca gcagcgtacc ctggccatcc
    59341 gggcttggat cgccagccac cacgttacgg atatccgcaa cttccggggt agcgacatcg
    59401 cgatcccagt tgatgccgaa gtagtgttta ccatcgatgt tagcaccagc agtgaaatca
    59461 ctcatcgccg caacggtacg gtcaatcacc accggaatcg gcatgtttac cggacccagt
    59521 gaacccggac cggctttaac cacggcacga atttcttctt cggtcgcgaa agtcagcggg
    59581 ctggcaacct gcggcagttt ttctgcttta acttcgttca gctcgtgatc accgcgcacc
    59641 agcagcgcaa ccagcgggaa gctactgcct tcaaccgctt taaccagcag agtcttaacc
    59701 gttttcttaa tcggcagatt gaactgttca accagttccg cgatggtttt cgcgttcggc
    59761 gtatcaacca gcgtcatttc ctgggtagca gcagcgcgcg gttctttcgg cgcgatagct
    59821 tctgccagct caatgttcgc tgcatagtca gaggtgtcgg agaagaccac atcgtcttca
    59881 ccgctctgcg ccagcacctg gaattcgtga gaggcgctgc cgccgataga accggtgtcg
    59941 gcctgtacgg cgcggaaatc cagccccatg cggctgaaga ttttgctgta ggccgcatac
    60001 attgcatcgt aggtttcctg cagggattcc tgagaagtat ggaaagagta agcatctttc
    60061 atcaggaatt cgcgggaacg catgacgccg aaacgcggac gcacttcgtc gcggaacttg
    60121 gtctggatct gatagaagtt cagcggcagc tgtttgtaag agctaagctc gttacgaatc
    60181 aggtcagtga taacttcttc atgagttggg ccgagtacga acggacgctc gccacggtca
    60241 acaaaacgca gcagttccgg accgtactgt tcccaacgac cactctcttg ccacaaatcg
    60301 gctggctgaa ccaccggcat cgacacctcg attgcaccgg cgttgttcat ctcttcacgc
    60361 acgatgtttt cgactttttt cagaacgcgc acgccggtcg gcagccaggt atataacccg
    60421 gaggccagct tgcggatcat cccggcgcgc agcatcagct gatggctgat cacctcggcg
    60481 tcggcaggtg tctccttgag agtggagagc aggtattggc tagtacgcat gttgttacgg
    60541 ttccagttgg aaggtagaac aggctcaagg cgagcctggg acaaaaaaag tgatttagtt
    60601 taccagtgca aaagaaatgt caaaagagaa gggcgtgaat ttaacgcggt tccagcgcaa
    60661 agacttcaaa acctgcgtcg gtgacgcgcc agcgaacgtt aaaatcatgt agccagacgg
    60721 cataggtttt gcccgtttcc tcacctttac gataggccgg gcgcgggtcc tgcgccagta
    60781 cttcgcggat aaacagcgtt aactgcggat aacgcttctc cagcgtcaaa agctgctttt
    60841 cgacctctgc ggtaaaactc accgccatct ctgcagctgg cgcactttgc gcatagctgg
    60901 cactggcatc gggaagcgat tcggcaaagg ggagatacgg tttgatatcc actaccggcg
    60961 taccatcgac cagatccagg ctgccgagct tcagaatcac gcagtcttta tggcaaacaa
    61021 cctctttcag ctctaccagc gacataccaa tcgggttggg gcggaaagtg gagcgcgtag
    61081 cgaaaacacc cattctggca ttaccgccaa gacgaggcgg acgcacagtc ggacgccagc
    61141 cgccttccat cgtttgatga aagacgaaaa ggatccataa atggctgaat gcttccaggc
    61201 cgcgaacggc gtcagcctgg ttgtagggag caatgagatg tagttcgccg ttggcgcttt
    61261 ttaccagacc tggctggcgc ggaacggcga atttttcttt atagggcgag cgaataacac
    61321 ctatttgctc gaactggaaa ctgctcattt cgccgtaatg ttaagcgcag aaccgataca
    61381 taaagcctga cgatagcagc ctggcgtacc gctggtaact tcgcagctat gcagtaatac
    61441 cgcattggct ttcattttcg aggcgttgat ttgcatccgc ttacgcgcgg ttggaatgct
    61501 cggcggagag tcctgattag aggcctggca agagtcgcca ctgacttcac cgagatcgcg
    61561 gaacggtttg ccgactaatt cttctgcatt ggtataaatt cggaccggcg tggcgcgcgg
    61621 cgctttcggt tttgcaggct ccgctttcgg ctggggtgca gtgctttgaa cgggttcgac
    61681 aggggatctg cttaacatgg aacagccgct tagcatgagt gctactaaac agatcggtaa
    61741 agcacgcata gtatttcctc aatgtatgat caaaacgtca atattgaatc aggagcttgt
    61801 aaaaatgaca agacgggcaa gcgcccgtcc tgaatgatat tacaaattgt ggaaacagcc
    61861 taaaaattac cagcctttaa cagcgccgcc gttaaacact ttgtttgctg cttcgtaaac
    61921 ttcgtcagac tgataagcct ggacgaattt cttcacgttc tcggcgtctt tgttatcttc
    61981 acgcgttacg atcaggttta cgtacgggga gtctttatct tcgacaaaga taccgttttt
    62041 cgctggagtc aggccaatct ggctggcata ggtggtgtta ataactgcca gagcgatttg
    62101 cgcgtcgtcc agagaacgcg gcagttgcgg tgcttccagt tcaacaattt tcagattttt
    62161 tgggttctca acaacatcaa gaacggtcgg cagcaggcca acgccatctt tcagtttgat
    62221 caagcccact ttttgcagca gcagcagtga acgaccaagg ttagttggat cgtttggcac
    62281 ggcaacctgc gaaccatcct gcagttcatc cagtgatttg attttcttgg agtaaccagc
    62341 aatcggataa acaaaagtgt tgcctactgc gaccagtttg tagccacgat ctttcagttg
    62401 ctgatcaagg tacggtttat gctggaaggc gttggcgtcg atatcgcctt tgctcaatgc
    62461 ttcgtttggc agaacatagt cgttgaaggt taccagctca acgtccaggc catatttgtc
    62521 tttcgcaact ttctgcgcga cttctgcaac ctgctgttcg gcaccaacaa tcacgccgac
    62581 tttaatgtgg tttggatctt tttcatcctg accgcagcct accagtgcca gtgatccaat
    62641 cagggctccc actgccgcaa aggttttgaa tttgaacgcc ataccttatt ccttcttcaa
    62701 ttatttatgt tgtgttgaac gttacttgcg agtgacagcc cggacgatgc ggtcgcctgc
    62761 gaactgaatt aaataaacca gaatgaccag caataccagt accgtattca tcaccgtcgc
    62821 gttatagccg atgtagccat actgatagcc aatctgacct aaaccaccgg caccgactgc
    62881 accacccatc gcggaataac cgaccagggt aatcagggtg atagttgccg cattcaccag
    62941 acccggcaac gcttccggta acagcacttt acggacgatc tgcattggcg tggcacccat
    63001 tgcgcgggaa gcttcaatta acccggttgg gatctccagc agagcgttct cgaccatacg
    63061 ggcaataaac ggtgctgcac caacggttaa cggaacaatt gctgcctgca aaccaatcga
    63121 tgtaccgaca ataacgcggg taaacggaat catccatacc agcaagataa tgaacgggat
    63181 ggaacggaaa atgttcacaa tcgcagaaac ggtacgatac agcttcgcgt tagcaataat
    63241 ttgccccgga cgcgtgacat aaagcaggac gccaaccggc agaccaacca caaacccaaa
    63301 aaatccggat acgaaggtca ttgccagcgt ttcccatacg ccacgaacca gcagccacat
    63361 catcggctca gacataaccc agtacctcta cttttacatg gtgttcctgc agccaggcaa
    63421 tggcggcttg cgtatcttgt tgtgtgccgt gcatttcagt cagcatgatg ccgaacttaa
    63481 cgccaccggc gtaatccatc tgcgcgctaa taatgttgtt gttgacgttg aaacgacgcg
    63541 cggtttcaga aagcagtggg gcatcgaccg attgaccggt aaactccaga cgcagcatcg
    63601 gcacgcagtc agtaaatggc tccgcttgca gacgttcctg gtaatcttcc gggatatcca
    63661 gatgcagggt tgactgaata aacttctgcg ccagcggcgt tttcggatgt gagaacactt
    63721 cacttaccgt gtcctgctcg atcagttcgc cattgctgat gaccgcaacg caatcacaaa
    63781 tacgcttcac aacgtccatt tcgtgggtga tcaacagaat cgtcaaaccc agacggcggt
    63841 taatgtcttt cagcagttcg agaatagaac gtgtcgttgc cgggtctagc gcgctggtgg
    63901 cttcatcaca cagcaatact ttgggattgc tggctaatgc acgggcaatc gccacacgtt
    63961 gtttctgccc accggaaaga ttcgacgggt agctatcatg cttatctcca agaccaacca
    64021 atgacagcaa ttccgtcacg cgacgtttga tctcgtcttt cggtgtattg tccagctcca
    64081 gcggcagagc cacgttgcca aaaacagtac gcgaagagag caggttaaaa tgctggaaaa
    64141 tcataccaat ctggcggcga gctttggtca actcggattc tgacagcgtg gtcagttcct
    64201 gtccatcgac cagcacgcta ccctcggttg ggcgctccag caggtttaca caacgtataa
    64261 gcgtactctt acccgcgcct gaggcaccga taacgccata aatttgtcca gctggcacat
    64321 gcaggctgac gttgttcaac gcctggatgg tgcgggtgcc ctggtggaac actttggtga
    64381 tattcgaaag ttttatcatt gattatttat tatcgtcatt aagttagtcg tggcatctcg
    64441 aatgcctgaa acgggcaacg ccgtcaatga aatggatgtt aaggcatcca gacgtctaaa
    64501 tcaatcaggt ttatgcgaag agcactttct tgcaggtcga aacatgcgat actagcgtca
    64561 catgccttat taaggagcta taaaaggtgg cgaagagcgt acccgcaatt tttcttgacc
    64621 gtgatggcac cattaatgtc gatcacggct atgtccatga gatcgacaac tttgaattta
    64681 tcgacggtgt tattgacgcc atgcgcgagc taaaaaaaat gggctttgcg ctggtggtag
    64741 tcaccaacca gtctggcatt gctcgcggta aatttaccga agcacagttt gaaacgctga
    64801 ccgagtggat ggactggtcg ctggcggacc gagatgtcga tctggatggt atctattatt
    64861 gcccgcatca tccgcagggt agtgttgaag agtttcgcca ggtctgcgat tgccgcaaac
    64921 cacatccggg gatgcttttg tcagcacgcg attatttgca tattgatatg gccgcttctt
    64981 atatggtggg cgataaatta gaagatatgc aggcagcggt tgcggcgaac gtgggaacaa
    65041 aagtgctggt gcgtacgggt aaacctatta cacctgaagc agaaaacgcg gcagattggg
    65101 tgttaaatag cctggcagac ctgccgcaag cgataaaaaa gcagcaaaaa ccggcgcaat
    65161 gattaaaaga tgagcggttg aaataaaaat gcatttttcc gcttgtcttc ctgagccgac
    65221 tccctataat gcgcctccat cgacacggcg aatgtgaatc acttcacaca aacagccggt
    65281 tcggttgaag agaaaaaatc ctg
//
LOCUS       DBFIFM010000004        65268 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.004, whole genome shotgun sequence.
ACCESSION   DBFIFM010000004 DBFIFM010000000
VERSION     DBFIFM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.004"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            98..1795
                     /gene="ypdA"
                     /locus_tag="N7C74_000196"
     CDS             98..1795
                     /gene="ypdA"
                     /locus_tag="N7C74_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HGU2430151.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1810..2544
                     /gene="ypdB"
                     /locus_tag="N7C74_000197"
     CDS             1810..2544
                     /gene="ypdB"
                     /locus_tag="N7C74_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HGU2430152.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            2557..3414
                     /gene="ypdC"
                     /locus_tag="N7C74_000198"
     CDS             2557..3414
                     /gene="ypdC"
                     /locus_tag="N7C74_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2430153.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRLVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(3417..5912)
                     /gene="ptsP"
                     /locus_tag="N7C74_000199"
     CDS             complement(3417..5912)
                     /gene="ptsP"
                     /locus_tag="N7C74_000199"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGU2430154.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIIRNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSEPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHGLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(5937..6974)
                     /gene="ypdE"
                     /locus_tag="N7C74_000200"
     CDS             complement(5937..6974)
                     /gene="ypdE"
                     /locus_tag="N7C74_000200"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU2430155.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFNYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(6974..8059)
                     /gene="ypdF"
                     /locus_tag="N7C74_000201"
     CDS             complement(6974..8059)
                     /gene="ypdF"
                     /locus_tag="N7C74_000201"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU2430156.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDATHTLTTIARQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKTSFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDEAARRV
                     ITEAGFSHYFGHNTAHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(8074..9321)
                     /gene="fryC"
                     /locus_tag="N7C74_000202"
     CDS             complement(8074..9321)
                     /gene="fryC"
                     /locus_tag="N7C74_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HGU2430157.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(9343..9669)
                     /gene="fryB"
                     /locus_tag="N7C74_000203"
     CDS             complement(9343..9669)
                     /gene="fryB"
                     /locus_tag="N7C74_000203"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGU2430158.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9888..10853)
                     /gene="glk"
                     /locus_tag="N7C74_000204"
     CDS             complement(9888..10853)
                     /gene="glk"
                     /locus_tag="N7C74_000204"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HGU2430159.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            11057..12313
                     /gene="yfeO"
                     /locus_tag="N7C74_000205"
     CDS             11057..12313
                     /gene="yfeO"
                     /locus_tag="N7C74_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HGU2430160.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            12428..12754
                     /gene="ypeC"
                     /locus_tag="N7C74_000206"
     CDS             12428..12754
                     /gene="ypeC"
                     /locus_tag="N7C74_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HGU2430161.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12894..14132)
                     /gene="mntH"
                     /locus_tag="N7C74_000207"
     CDS             complement(12894..14132)
                     /gene="mntH"
                     /locus_tag="N7C74_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HGU2430162.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            14468..15670
                     /gene="nupC"
                     /locus_tag="N7C74_000208"
     CDS             14468..15670
                     /gene="nupC"
                     /locus_tag="N7C74_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HGU2430163.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ACEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WIGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15720..17909)
                     /gene="pdeA"
                     /locus_tag="N7C74_000209"
     CDS             complement(15720..17909)
                     /gene="pdeA"
                     /locus_tag="N7C74_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HGU2430164.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEVLARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(18117..18192)
                     /locus_tag="N7C74_000210"
     tRNA            complement(18117..18192)
                     /locus_tag="N7C74_000210"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18157..18159),aa:Ala,seq:ggc)
     gene            complement(18232..18307)
                     /locus_tag="N7C74_000211"
     tRNA            complement(18232..18307)
                     /locus_tag="N7C74_000211"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18272..18274),aa:Ala,seq:ggc)
     gene            18528..18887
                     /gene="yfeC"
                     /locus_tag="N7C74_000212"
     CDS             18528..18887
                     /gene="yfeC"
                     /locus_tag="N7C74_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HGU2430165.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18889..19281
                     /gene="yfeD"
                     /locus_tag="N7C74_000213"
     CDS             18889..19281
                     /gene="yfeD"
                     /locus_tag="N7C74_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HGU2430166.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(19321..20529)
                     /locus_tag="N7C74_000214"
     CDS             complement(19321..20529)
                     /locus_tag="N7C74_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HGU2430167.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKELERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYLNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCTANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            20537..20962
                     /gene="tnpA"
                     /locus_tag="N7C74_000215"
                     /pseudo
     CDS             20537..20962
                     /gene="tnpA"
                     /locus_tag="N7C74_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(21080..22495)
                     /gene="gltX"
                     /locus_tag="N7C74_000216"
     CDS             complement(21080..22495)
                     /gene="gltX"
                     /locus_tag="N7C74_000216"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HGU2430168.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYFQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            22754..22829
                     /locus_tag="N7C74_000217"
     tRNA            22754..22829
                     /locus_tag="N7C74_000217"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22787..22789,aa:Val,seq:tac)
     gene            22874..22949
                     /locus_tag="N7C74_000218"
     tRNA            22874..22949
                     /locus_tag="N7C74_000218"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22907..22909,aa:Val,seq:tac)
     gene            22996..23071
                     /locus_tag="N7C74_000219"
     tRNA            22996..23071
                     /locus_tag="N7C74_000219"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23029..23031,aa:Val,seq:tac)
     gene            23076..23151
                     /locus_tag="N7C74_000220"
     tRNA            23076..23151
                     /locus_tag="N7C74_000220"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23109..23111,aa:Lys,seq:ttt)
     gene            23271..23612
                     /locus_tag="N7C74_000221"
     CDS             23271..23612
                     /locus_tag="N7C74_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HGU2430169.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQHI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIGIYI"
     gene            complement(23603..24529)
                     /gene="yfeR"
                     /locus_tag="N7C74_000222"
     CDS             complement(23603..24529)
                     /gene="yfeR"
                     /locus_tag="N7C74_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2430170.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     VILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNTLMAGREGDPLYQI"
     gene            24619..25617
                     /gene="yfeH"
                     /locus_tag="N7C74_000223"
     CDS             24619..25617
                     /gene="yfeH"
                     /locus_tag="N7C74_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HGU2430171.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(25614..25832)
                     /gene="ypeB"
                     /locus_tag="N7C74_000224"
     CDS             complement(25614..25832)
                     /gene="ypeB"
                     /locus_tag="N7C74_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HGU2430172.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(25834..27849)
                     /gene="ligA"
                     /locus_tag="N7C74_000225"
     CDS             complement(25834..27849)
                     /gene="ligA"
                     /locus_tag="N7C74_000225"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HGU2430173.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTYCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(27920..28918)
                     /gene="zipA"
                     /locus_tag="N7C74_000226"
     CDS             complement(27920..28918)
                     /gene="zipA"
                     /locus_tag="N7C74_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HGU2430174.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            29148..29909
                     /gene="cysZ"
                     /locus_tag="N7C74_000227"
     CDS             29148..29909
                     /gene="cysZ"
                     /locus_tag="N7C74_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HGU2430175.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            30094..31065
                     /gene="cysK"
                     /locus_tag="N7C74_000228"
     CDS             30094..31065
                     /gene="cysK"
                     /locus_tag="N7C74_000228"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HGU2430176.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            31449..31706
                     /gene="ptsH"
                     /locus_tag="N7C74_000229"
     CDS             31449..31706
                     /gene="ptsH"
                     /locus_tag="N7C74_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HGU2430177.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            31751..33478
                     /gene="ptsI"
                     /locus_tag="N7C74_000230"
     CDS             31751..33478
                     /gene="ptsI"
                     /locus_tag="N7C74_000230"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HGU2430178.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            33519..34028
                     /gene="crr"
                     /locus_tag="N7C74_000231"
     CDS             33519..34028
                     /gene="crr"
                     /locus_tag="N7C74_000231"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HGU2430179.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(34071..34922)
                     /gene="pdxK"
                     /locus_tag="N7C74_000232"
                     /gene_synonym="thiJ"
     CDS             complement(34071..34922)
                     /gene="pdxK"
                     /locus_tag="N7C74_000232"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HGU2430180.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMLVVVVTADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            35027..35394
                     /gene="yfeK"
                     /locus_tag="N7C74_000233"
                     /pseudo
     CDS             35027..35394
                     /gene="yfeK"
                     /locus_tag="N7C74_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
     gene            complement(35397..36308)
                     /gene="cysM"
                     /locus_tag="N7C74_000234"
     CDS             complement(35397..36308)
                     /gene="cysM"
                     /locus_tag="N7C74_000234"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HGU2430181.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMADRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITYFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(36442..37539)
                     /gene="cysA"
                     /locus_tag="N7C74_000235"
     CDS             complement(36442..37539)
                     /gene="cysA"
                     /locus_tag="N7C74_000235"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HGU2430182.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIEPRDEELALAQSA"
     gene            complement(37529..38404)
                     /gene="cysW"
                     /locus_tag="N7C74_000236"
     CDS             complement(37529..38404)
                     /gene="cysW"
                     /locus_tag="N7C74_000236"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HGU2430183.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEENHEH"
     gene            complement(38404..39237)
                     /gene="cysT"
                     /locus_tag="N7C74_000237"
     CDS             complement(38404..39237)
                     /gene="cysT"
                     /locus_tag="N7C74_000237"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HGU2430184.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(39237..40253)
                     /gene="cysP"
                     /locus_tag="N7C74_000238"
     CDS             complement(39237..40253)
                     /gene="cysP"
                     /locus_tag="N7C74_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HGU2430185.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(40411..41202)
                     /gene="ucpA"
                     /locus_tag="N7C74_000239"
     CDS             complement(40411..41202)
                     /gene="ucpA"
                     /locus_tag="N7C74_000239"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HGU2430186.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(41331..42188)
                     /gene="murR"
                     /locus_tag="N7C74_000240"
     CDS             complement(41331..42188)
                     /gene="murR"
                     /locus_tag="N7C74_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HGU2430187.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATAQHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGCDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            42352..43248
                     /gene="murQ"
                     /locus_tag="N7C74_000241"
     CDS             42352..43248
                     /gene="murQ"
                     /locus_tag="N7C74_000241"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HGU2430188.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            43252..44676
                     /gene="murP"
                     /locus_tag="N7C74_000242"
     CDS             43252..44676
                     /gene="murP"
                     /locus_tag="N7C74_000242"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HGU2430189.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PDIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            44681..45985
                     /gene="pbp4b"
                     /locus_tag="N7C74_000243"
     CDS             44681..45985
                     /gene="pbp4b"
                     /locus_tag="N7C74_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HGU2430190.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMAQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDLIAKYISGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     THWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(46043..46942)
                     /gene="yfeX"
                     /locus_tag="N7C74_000244"
     CDS             complement(46043..46942)
                     /gene="yfeX"
                     /locus_tag="N7C74_000244"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HGU2430191.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNIWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(47038..47613)
                     /gene="yfeY"
                     /locus_tag="N7C74_000245"
     CDS             complement(47038..47613)
                     /gene="yfeY"
                     /locus_tag="N7C74_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HGU2430192.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADAVDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(47674..48123)
                     /gene="yfeZ"
                     /locus_tag="N7C74_000246"
     CDS             complement(47674..48123)
                     /gene="yfeZ"
                     /locus_tag="N7C74_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HGU2430193.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            complement(48110..48535)
                     /gene="ypeA"
                     /locus_tag="N7C74_000247"
     CDS             complement(48110..48535)
                     /gene="ypeA"
                     /locus_tag="N7C74_000247"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HGU2430194.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            48749..49618
                     /gene="amiA"
                     /locus_tag="N7C74_000248"
     CDS             48749..49618
                     /gene="amiA"
                     /locus_tag="N7C74_000248"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HGU2430195.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            49622..50521
                     /gene="hemF"
                     /locus_tag="N7C74_000249"
     CDS             49622..50521
                     /gene="hemF"
                     /locus_tag="N7C74_000249"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HGU2430196.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREGGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGKDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(50527..51579)
                     /gene="eutR"
                     /locus_tag="N7C74_000250"
     CDS             complement(50527..51579)
                     /gene="eutR"
                     /locus_tag="N7C74_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HGU2430197.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(51625..52125)
                     /gene="eutK"
                     /locus_tag="N7C74_000251"
     CDS             complement(51625..52125)
                     /gene="eutK"
                     /locus_tag="N7C74_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HGU2430198.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGVTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(52138..52797)
                     /gene="eutL"
                     /locus_tag="N7C74_000252"
     CDS             complement(52138..52797)
                     /gene="eutL"
                     /locus_tag="N7C74_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HGU2430199.1"
                     /translation="MPALDLIRPSVTAMRVIASVNADFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAQDTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(52807..53694)
                     /gene="eutC"
                     /locus_tag="N7C74_000253"
     CDS             complement(52807..53694)
                     /gene="eutC"
                     /locus_tag="N7C74_000253"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HGU2430200.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTTCAAPVTSESCA
                     LDLGSAEAKVWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTLALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(53715..55076)
                     /gene="eutB"
                     /locus_tag="N7C74_000254"
     CDS             complement(53715..55076)
                     /gene="eutB"
                     /locus_tag="N7C74_000254"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HGU2430201.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELCSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     CIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(55088..56491)
                     /gene="eutA"
                     /locus_tag="N7C74_000255"
     CDS             complement(55088..56491)
                     /gene="eutA"
                     /locus_tag="N7C74_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HGU2430202.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDVRSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(56488..57714)
                     /gene="eutH"
                     /locus_tag="N7C74_000256"
     CDS             complement(56488..57714)
                     /gene="eutH"
                     /locus_tag="N7C74_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HGU2430203.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKDDATAAKTEAEAQS"
     gene            complement(57814..59001)
                     /gene="eutG"
                     /locus_tag="N7C74_000257"
     CDS             complement(57814..59001)
                     /gene="eutG"
                     /locus_tag="N7C74_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HGU2430204.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQLVGLYA
                     AAQ"
     gene            complement(58991..59827)
                     /gene="eutJ"
                     /locus_tag="N7C74_000258"
     CDS             complement(58991..59827)
                     /gene="eutJ"
                     /locus_tag="N7C74_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HGU2430205.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTFAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(59838..61241)
                     /gene="eutE"
                     /locus_tag="N7C74_000259"
     CDS             complement(59838..61241)
                     /gene="eutE"
                     /locus_tag="N7C74_000259"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HGU2430206.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     IAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAGKIA
                     AAIGLKVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAVDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(61253..61540)
                     /gene="eutN"
                     /locus_tag="N7C74_000260"
     CDS             complement(61253..61540)
                     /gene="eutN"
                     /locus_tag="N7C74_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HGU2430207.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPHGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(61647..61940)
                     /gene="eutM"
                     /locus_tag="N7C74_000261"
     CDS             complement(61647..61940)
                     /gene="eutM"
                     /locus_tag="N7C74_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HGU2430208.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(61979..62995)
                     /gene="pta"
                     /locus_tag="N7C74_000262"
     CDS             complement(61979..62995)
                     /gene="pta"
                     /locus_tag="N7C74_000262"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGU2430209.1"
                     /translation="MIIERCCELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(62992..63723)
                     /gene="eutT"
                     /locus_tag="N7C74_000263"
     CDS             complement(62992..63723)
                     /gene="eutT"
                     /locus_tag="N7C74_000263"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HGU2430210.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPLGCQAIVGLSDEDLHRLSHQPLRYLGH
                     DHLVPEASHGRDAALLNLLRTKVREAETVAAQVFITRSFEVLRPDILQALNRLSSTVY
                     VMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(63720..64421)
                     /gene="eutQ"
                     /locus_tag="N7C74_000264"
     CDS             complement(63720..64421)
                     /gene="eutQ"
                     /locus_tag="N7C74_000264"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HGU2430211.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDELIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(64396..64875)
                     /gene="eutP"
                     /locus_tag="N7C74_000265"
     CDS             complement(64396..64875)
                     /gene="eutP"
                     /locus_tag="N7C74_000265"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HGU2430212.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(64888..65223)
                     /gene="eutS"
                     /locus_tag="N7C74_000266"
     CDS             complement(64888..65223)
                     /gene="eutS"
                     /locus_tag="N7C74_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HGU2430213.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
BASE COUNT        15344 a        18013 c        16541 g        15370 t
ORIGIN      
        1 cccttttaaa attgtggttt ttcctcaaaa acggcctgct ggtcatcagc cttcaggtta
       61 cctatcatag aggtttaatc cttattcaga gtcacccgtg cacgaaatat tcaacatgct
      121 gctggcggtc ttcgatcggg cagcgttaat gcttatctgc ctgttctttc tcatccgtat
      181 ccgcctgttt cgcgaactgt tgcacaagtc ggcacactca ccgaaagagt tgctcgccgt
      241 taccgccatt ttctcgctgt tcgccctctt cagcacctgg tccggcgttc ccgtagaagg
      301 ctcgctggtg aacgtacgta ttatcgcggt gatgtccggc gggattctgt ttggcccgtg
      361 ggtgggcatc attaccggcg tgattgcggg tattcatcgt tatttaattg atatcggtgg
      421 cgtgacggcg atcccctgct ttatcaccag cattctggct ggttgtatat cgggctggat
      481 caacctgaaa atccccaaag cacagcgctg gcgcgtcggt attctcggcg gcatgttgtg
      541 tgaaacgttg accatgattc tggtgattgt ctgggcacca actaccgcgt tggggatcga
      601 tatcgtctct aaaatcggga ttccaatgat cctcggcagc gtctgtatcg gctttattgt
      661 gcttctagtg caaagcgttg agggcgaaaa agaggccagc gccgcgcggc aggccaagtt
      721 ggcgctggat atcgccaaca aaacgctacc tctgtttcgc catgtcaata gcgagtcatt
      781 acgcaaagtt tgtgaaatta ttcgcgatga cattcacgcc gatgcggtgg cgattaccaa
      841 taccgatcat gtgctggcct atgttggtgt gggtgaacat aactatcaga atggcgatga
      901 cttcattagc ccgactaccc gtcaggcgat gaattacgga aaaatcatca ttaaaaacaa
      961 tgatgaagcc caccgcacac cagagattca ttccatgctg gtgatcccat tgtgggagaa
     1021 aggcgttgtg accggaacgc tgaaaattta ctactgccac gcgcatcaga taacctcgtc
     1081 attacaggaa atggcggttg gtctgtcgca aatcatctcc acgcaactgg aggtttcacg
     1141 cgccgagcag ctacgtgaaa tggcaaataa ggcagagctt cgcgccctgc aaagcaaaat
     1201 taatccccat ttcctgttta acgctttgaa cgctatttca tcgtcgatcc gtctgaatcc
     1261 ggataccgct cgccagctga tctttaatct gtcgcgttat ctgcgctata acattgaatt
     1321 aaaagacgat gagcaaatcg atatcaaaaa agagctgtat caaattaaag actatattgc
     1381 cattgagcag gcccgctttg gcgacaagct gacggttatc tatgatattg atgaagaggt
     1441 gaattgctgc attcccagcc tgctgatcca gccgttggtg gagaatgcca ttgtccacgg
     1501 tatccagcct tgcaaaggta aaggcgttgt caccatcagc gttgcagagt gcggcaatcg
     1561 ggtacgcatt gcggtgcggg ataccgggca cggcatcgat ccaaaggtga ttgagcgggt
     1621 cgaagcgaat gaaatgccgg gcaataaaat tggcctgctg aatgtccatc atcgcgtgaa
     1681 gttattgtat ggcgaggggc tgcatatccg ccgcctggaa ccaggaacgg aaattgcgtt
     1741 ttacattcct aaccaacgca ccccagtcgc ctcacaggct acgttattgc tttgagccgg
     1801 agtgatattg tgaaagtcat cattgttgaa gacgaattcc tggcacaaca ggaactgagc
     1861 tggctaatta aagagcacag ccagatggag attgtcggca cctttgacga cggtctggac
     1921 gtgttgaagt ttttgcagca taaccgcgtc gacgccattt ttctggatat caatattccg
     1981 tcgctggatg gtgtgttgct ggcacaaaac atcagccagt tcgcccataa accgtttatt
     2041 gtgttcatca ccgcgtggaa agaacatgcg gtagaagcgt ttgaactgga ggcgtttgac
     2101 tacattctaa aaccgtatca ggagtcacgc attaccggga tgctgcaaaa actggaagca
     2161 gcctggcaac aacagcagac cagcagcacg cctgccgcga cggtaacgcg tgagaatgac
     2221 accattaatc tggtgaaaga tgagcgaata atcgtcacgc caattaacga tatctattac
     2281 gccgaagcgc acgagaaaat gacctttgtt tatacgcggc gtgaatccta cgtaatgccg
     2341 atgaacatta ccgaattttg cagcaaactg ccgccgtcgc attttttccg ctgccatcgc
     2401 tcattttgtg tcaatctgaa caaaatccgc gaaatcgaac cgtggtttaa taacacctat
     2461 attctgcggc tgaaagatct ggattttgaa gtgccggtca gccgcagcaa agttaaagaa
     2521 ttccgccagt taatgcatct ttaatcggga actttcatga aagcaccagg cttgcccgcc
     2581 gatcaacaat ttttcgccga tctgttcagc ggcctggtgc ttaacccgca actactgggg
     2641 cgcgtctggt ttgccagcca gcctgcctcg ttgccggtgg gcagtttatg tattgatttt
     2701 ccccgtctgg atatcgtgct gcgcggcgaa tacggcaatc tgctggaagc aaagcagcaa
     2761 cgtttggtgg aaggagaaat gctgtttatt ccggcgcgcg cggctaattt accggtcaac
     2821 aacaaaccgg tgatgctgtt aagcctggtg ttcgctccga cctggcttgg gttatcgttt
     2881 tacgatagcc gcaccacgtc attgttgcat cctgctcgcc agatccagct tcccagcctg
     2941 caacgcggtg aaggtgaagc gatgcttacc gccctcaccc atcttagccg ttcgccgctg
     3001 gagcaaaata tcattcagcc gctggtgtta agtttgctgc atctttgccg taacgtggtg
     3061 aatatgccgc cgggcaattc gcagccgcgc ggcgattttc tctatcacag catttgtaac
     3121 tgggttcagg ataattatgc ccagccgctc acccgcgaga gcgtggcgca gttttttaat
     3181 atcacgccca atcatctgtc aaaactgttt gctcagcatg gaacgatgcg ttttatcgag
     3241 tatgtgcgtt gggtgcgaat ggcgaaggcg aggatgattt tgcagaaata tcatttgtca
     3301 attcatgaag tggcacagcg ttgcggtttt ccggatagcg actatttttg tcgcgttttc
     3361 cggcgtcagt ttggtctgac gccgggagag tacagcgccc gttttcaggg ctaacgtcag
     3421 aaggttaatt ctgtttccag cagcgtcagg atactttgtg catcttgcgc ggcaaacaac
     3481 gactggcgga agtttttatt caccagttta cgcgccagct gcgagaagac tttcacatga
     3541 ttcatccctt cgttagcacc cagcgtcagc atgatcacca gttcgacttc gcccatttct
     3601 gactgccagt caaccggttt cgccagccgg gcaatgctga tactggaatg acggatccac
     3661 tgagatttgg tgtgcgggat cgccacgcca aaaccaacgc cagtggtgac aatctcttcc
     3721 cgctgccaga catcctcttc cagctcgaac ggatgttcag tgcgcccgtt aacgccgagg
     3781 ttaccgcaca ggaactggat cgcctgctct ttattgctaa aggcctgatc aacgaagata
     3841 ttctccagcg ccagcagcgg gcgaacgtct tcttccggcg taaaggcggt gagtaacgct
     3901 tcaatttcct gcgcgctgcg acattcacac gcctgacgtg ccagttcccg acacgcctcg
     3961 ctatccagtt gacgaagctg gcttttcacc gccggaatgc gcgggctact catactcagc
     4021 tcatccaggc ccagcccaag cagtagcggc agataacggc tttcaccgcc cagttcaccg
     4081 caaatgccta cccatttgcc ccgctgatgc gcagtggtaa ctatttgctg caacatgcgc
     4141 aggaacgatg gcgtaatcgg gttatatagc ggcgatacgc gcgggttatt acgatcgacc
     4201 gcatacagat actgggtcat atcgttggag ccgatactga agaaatcgac ctcatcgcag
     4261 aagtgatcga tgatgtagca caccgacggc acttccacca tgatacccag cgtaatcgtc
     4321 tctgcatgac gcaggccatc gcgcttaagc tcaacgatcg ctttttggat ctcgcccttc
     4381 acccataaga tctgatcgag gccgtggacc atcgggatca tcaactgggc gttgccgaaa
     4441 ctggcggcgc gcaaaatggc ccgcagttga gtgcggaaca ggccagcaaa ttccgggtaa
     4501 atacgtaccg cgcgatagcc gaggaacggg ttctcttcct ggggaatatt cagataagga
     4561 atgcttttat cgcctccgat gtccattgtg cggaagataa tcggcttgtc gcccgctgcc
     4621 agtagcacct gctggtaggc ttcaaattgc tcctgctcgt ccggctcgct gtcgcgatcc
     4681 atatagagca tttcggtacg gaacagcccg acgccttccg cgccgttggc aaacgcgcca
     4741 ggcgcttcca gagcggtgcc aatattcgcc gcgatatcaa tacgcttgtt atcacgggaa
     4801 taagccagct gcgcggcagc ctgcgcctgt tgtttttggc gtttatccgc cagcgtctgc
     4861 gcgacctgat aataaccgct caccgcgtca ttcgggttaa tcgccagcac gccgcactgg
     4921 gcgtcaagca ctgcaggttg cccggcataa cgcgcgatcg catccagcgg caggccgctc
     4981 agtaccggga tcgccgaggc gcgggccaga atcagggtat gcgaggtgcg cccggttttc
     5041 tccagaatca tgcccgcaag attttttaaa tccaggctta aaaactggct tggggttaaa
     5101 tcctcggcca ccagaatagt cggtttttcc agcaccagat tgttgcgcgg cttcagttcc
     5161 ggccaggtaa tatgcagcaa ctgttcgcta atatcgcgaa tgtcgctaac acgttcgcgc
     5221 agataatcgc tggcagaagc agaaagtttg gcgcaaacct gctccatatt tctgatgatc
     5281 gccgccccca gcccctgatg ctgttctgtc atcaggcgac ggatattgcc tgcaaattca
     5341 tcatcctgaa tcagcgacaa atgggcgctg aggatagttt tgctttcgcc gtcacgctca
     5401 cgcaattgct ggttcagttg ctcggcaagc gttgccaggc tgtgctccag ccgggtggaa
     5461 tcttgcgcac tggcggggat tgcccgataa ctgtcgaggc tgtcgctttg taacagggtc
     5521 agcgtaccca cgccgacgcc gcttgccagc acattgccgt acagtaaatc cgggttaagg
     5581 cggcttaatg aacgcggcag cggatgcgcc gtcagttctg cctgcgtagg ctgaacgctg
     5641 tcgctgtcga taaagcgcac ctggatgtac tcttccagca cgcgccgcgc ctgctcttca
     5701 tcgctgccgc taatgttcag gctgcaactg tcattaaata gggtgccggt gccaatcagc
     5761 gccagcgagc ttttggcatc ggcctttgcg ttctggcgat ggttaataaa agtgatttcg
     5821 ctttgccact ggctgcactg ttctttaagt tcccacgccg gacgagcatg tagaccgtta
     5881 ggcaaaggac agagaaattg aatcgttaac ataaccgctc cgggttaatc aggagatcat
     5941 ctgaaatccg tcagttgaac aaccgtctca cgcgtaagac gttgaataag ggcagataaa
     6001 agttgctcca tctgcaaaat gtcacggcaa tcggcaatcg acgcggcgca atgtccgtgg
     6061 cgggtggcag gccccatcac cactgtgggt acgccagtac cggttaagtg caccgctcca
     6121 ccgtccgtgc cgccgttact gaacatatcc gcctgtaacg gcacgccaat ttctgccgcc
     6181 acggtttcga tccaggcggt gagttttggc ggcgcaatca gtgacttgtc gcttaacacc
     6241 agcatcgggc cgttaccaat ctggcgatgg ttagccgcgc cataattaaa gtttttcgcc
     6301 cagcaggcgg tatcaaggac aatggcgacg tccggcgaca ccgcgcgggt ggcagtttgc
     6361 ccgccgcgta accccacctc ttcgctggaa ctggccacca gccacacttc cgcaggcagt
     6421 tcagcgctgt gtagttcgcg cagtaacgtc accagcaggt agcaaccgag gcggtcatca
     6481 aaggctttcc ccatcacccg ctggtggggg agaacctgaa aagtggtatc gaacgtgacg
     6541 cgatcgcctg gacgaattcc cgcctgcatc acttcgtcat acgagcgcgc gccaatatcc
     6601 acgcgcatgg cgctgacatc attcccctgc cggtcgccgt caagcaggcc tggaattttg
     6661 cactcttcac gggtggtgat gcgcaccggc tgcagctggc gggcagccat gcgtacattg
     6721 ccaaccggca gcacatcaat cgccccttcg cgggagatgc tgcgcaccat aaatcccact
     6781 tcgtccatat gcgcacagat catcaccttc ggacctgtcg attcattgag gcggatcagc
     6841 accgatccca gcccatcaaa tcgcacttct ttttgcaggc gatccgcttc ttccagcagg
     6901 atctgccgca cttcctgttc cgaggaggcg atcgcatctg cctcgctcaa cgctttcaat
     6961 agcgataaat ccattatgcc tctcccgtga gcaacactgt tttcggcatg gcgtagagca
     7021 cttctgcgcc ttgcggggtg accagcacaa catcctcgat gcgcacgccc ccttgccctg
     7081 gcaaataaat ccccggctcc acggtcagta acatgcctgg ctgtagcgtc gtggtgtccc
     7141 gcggtgaaaa acgcggatct tcatgaactt caatgccgat agcatgagcg gtgttatgac
     7201 cgaaatagtg gctaaaacct gcctcggtaa tcacccgacg cgcggcttcg tcaacctgct
     7261 ggcagcgcac gccggggcga attgcagaga ttgctgcgag ctgtgcctgc aaaacaatct
     7321 gatagacgtt aaacagcggg tgagattcgg cgctcacccc ttcgccattc accagcaagg
     7381 tgcgcgtcat atcagagcag tagccctgat agagcgcacc gaaatcgaga gtgacaaact
     7441 cgcccgctgc aacaatcttg tcgctggctt tgccgtgcgg cagcgcccca cgccagccac
     7501 tggcgacaat ggtgtcaaaa gaggtttttt ctgcgccctg ctggcgcata aaccactcca
     7561 gttcagcggc tatctcgcgc tcgctcatcc ccgcctgaat aaagcggcga atatgctctg
     7621 caccgcgatc ggcaatccca caggcaaggc ggattttctc cacctcctct ggcgttttga
     7681 tttgccgcag cacatccggc gtggcgctaa ccagtttcgc attgagttca gactgccagc
     7741 gatgcgcggt ttcccaactc acctgctggc cttcaaaacc gagcgtttgc aactgctcat
     7801 cggcaatgat ttgcctggcg atggttgtaa gcgtgtgcgt cgcgtcaagc aaatgcagct
     7861 ggtaaccttg cgtgcgggct tctacatccg cgtaatagcg cgagtccacc agaatgtgcg
     7921 cactttcacg gctaatcagc acatagcctg atccggtgga gatccccaga tgcggctgtt
     7981 tgttctgccg tgaggagaga agcaccgcat ccagttgttg cgccttaagc cagtcgcgca
     8041 gcgaagcgag taatgtcatc tggattttcc ttcttacagg ctatcgatta acaatttgcc
     8101 tttacggaac atcatcagac gcaggaacac caccatcagc gcagttatga ctgcccccag
     8161 cgcgatcccc gccatataga cgcccaggtt agtgaccagc ggccatgccc agatagcgga
     8221 ttccgggaac cattgcactg cgcccagcca gacagcggcg gttgagccga caatcgcgcc
     8281 gaccatatac gacggaatgg cggtgatggg gctttccagc gcaaacggaa tcgcaccctc
     8341 actgatcccc ataaaggcaa ggaacatcgc ggtcttaccc tgcggataaa gttgtgcgtt
     8401 gaacaggcgt ttgccggtta aacgacggtc aataatggtc gccagaccca gaccaatcgg
     8461 cggaataacg atagcgatag aacgagcggt gaccggtaat acgtggtcag tggtaaagct
     8521 gaaggcaaca aaacctgcgg ctttgttgat cgggccacca aggtcgattg ctgtcgccgc
     8581 ggcgatcccc attgcgtaca tcagcgcgcc cttctcacct gcggcagtca gcacagtacg
     8641 gataccgccg ttgatccagc ccccaaaggg ggtgatgacg tagtacatcg ccagcatgac
     8701 aaaaatagcg gaaagaatcg gtagcaaaaa tgtggttttg aacgctaaca ggaaatccgg
     8761 taactggatt ttctggttca tccatttcac aaggtaaccg gcaacgatag aaataatcag
     8821 cgcaccaatg aaggtggacg gcaccggcga agaggtcgcc cattgcatag tgctgggatc
     8881 gaagttcagc agttgcgtcg gctgggtaga catcaagccg ccgataaaac cagccggaaa
     8941 cgccagtttg ccaccgatag agttcgccac aaaagcagca aacatcggaa tggcaaaacc
     9001 aaacaacacg ccgccgaagg actgcgacaa ccaggcaaat ttcagtaagg agaggtcgaa
     9061 accggagaat ttcccgctat taagagcatc catgatgccg atctcagcag gaattttaag
     9121 ccagctataa gcaatcaact ggctgaaagc gaggatcaca ccgcccataa ttaaggtcgg
     9181 caccatgcgt gaaatccctg acatcacatg ctgcgggagt tcaccccaga agctgctttt
     9241 agaggcctgc ggattcttaa ctgccgctgc cgcaccggat gcgccaggca caacggttgc
     9301 actgcgtttt ttaatggcca taacaattat ccctgtcgaa tattactgtt gttcagaggc
     9361 aatcatctct tcgatttctt tgatgatgcc cgcagcgttt ttaattgcgt cctgcaaagt
     9421 gatttcataa acgtcgcgtg attcgaaacg ttcgttatct tccggggtaa ctgccacgga
     9481 gtggatgatg atggtcgctt cggcgatatc ctgcgccgtc aggcgattct ggataccgtc
     9541 cgcgccctgg gtttcaattt tcacttcata accggcttct accgccgctt cttccagcgc
     9601 ctgagcggcc ataaaagtgt gagccaggcc cattgggcag gcacataagg caatcagttt
     9661 cttactcatc gttaattcct cgttcagtga tagttgacga gtgaaattat gaacgtgcaa
     9721 aacaaatcgc cgttaccaga caaataatgc atttactgga gaattaatcc cgctgtacgg
     9781 aagtgtgacg caggtcggcc ttgtgagggg acacaaagaa aagttgaatt aactgtatga
     9841 tttaaaagat tatcgggaga gttacctccc gatataacag gaaggattta cagaatgtga
     9901 cctaaggtct ggcgtaaatg tgcaccggaa ccgagaagac ccggattgtc atggacgatg
     9961 agatacaccg gaatatcatg gacatattct ttaaagcgcc ctttatcttc aaatgcggca
    10021 cggaaaccag aggctttgaa gaactcaagg aagcgcggca cgataccgcc cgcaataaac
    10081 acgccgccaa atgtcccgag attgagcgcc agattgccgc caaaacggcc cataatgacg
    10141 caaaacagcg acaacgcacg gcggcaatcg gtgcagctgt cagccagcgc gcgttcggta
    10201 atatcttttg gcttgagatt ttctggcagg cggttgtcgg ctttaacaat tgcgcgatac
    10261 aaattcacca gtcctgggcc agaaagcacg cgttccgccg aaacatgacc aatttccgca
    10321 cgcaatattt cgaggataat ggcctcttct tcactattcg gcgcaaaatc aacgtgaccg
    10381 ccttcgcccg gcaagcttac ccaacgctta tcgacatgga ccagatgcgc aaccccaagc
    10441 cccgttccgg caccgtaaac cgcaataggc ttaccttcta ccggttctgc gccaccaaac
    10501 tgaatcagat gctctttttt cagcatcggg atcgccatcg atacagcggt aaaatcgtta
    10561 ataatttcca gatggctaaa accgagattc tttttcattt cggcaattga gaacgcccag
    10621 gtatggttgg tcattgccac ccagtctccg gtaatcgggc aagcgatggc aatacagccg
    10681 tctttcacct cgaccttatg ttcttcaaga taaacgcgaa tgaccgcttc gaggctgggg
    10741 taatcaagcc ctgaataggt cttagcctgc gagatttcac cactggcaat atcacacaga
    10801 gcaagacgtg cgttggtgcc gcccacatca ccgactaatg catactttgt cattcttcaa
    10861 ctgctccgct aaagtcaaaa taattctttc tcacactgta aatacctggg ggcataacaa
    10921 caacgccgga aaggcaggct ccctgtaaat atcgatctgg gtcacacaat tactttatcg
    10981 tttcagcacc aattgcagcg atgccttttt gcaagctggg caaactaagt atctgacccc
    11041 gcataaggaa tagaacatgc tccatccgcg agccagaacc atgttgttat tatcgctccc
    11101 cgccgtggca attgggattg cgtccagtct tattctgatt gtggtgatga aaatcgcctc
    11161 ggtattacag aatttgctct ggcaacgact gccgggaact ctggggatag cccaggattc
    11221 acccctctgg atcatcggtg tattaacgct aacgggtatt gcggtggggt tggttatccg
    11281 tttcagccag ggtcatgccg gaccagaccc cgcctgtgaa ccgctgatcg gcgcaccggt
    11341 tccgccctct gcgctaccag gacttatcgt agcattaatt ctcggtcttg ctggcggcgt
    11401 cagcctgggg ccggaacatc cgatcatgac cgtcaatatc gcccttgcgg ttgccattgg
    11461 cgctcgtctg ttaccgcgcg tcaaccgaat ggagtggact attttagcct ctgccggaac
    11521 catcggcgcg ctgtttggta cacctgttgc ggcggcgttg atattttcgc aaaccttaaa
    11581 tggcagtagt gaagttccgc tatgggatcg tctttttgcg ccgttaatgg cggcagcagc
    11641 tggtgcactt actaccggat tatttttcca tccccatttt tcactgccca ttgcgcatta
    11701 cggacagatg gaaatgaccg atattctcag cggtgcaatt gtcgcggcga ttgccatcgc
    11761 agcagggatg gtcgccgtat ggtgcttacc acggttgcac gcgatgatgc atcaaatgaa
    11821 aaatccggtg ctcgtgctgg gtattggcgg atttattctc ggtattctgg gggttattgg
    11881 tggaccagtt tcgctgttta aagggctgga tgagatgcag cagatggtgg caaatcaggc
    11941 tttcagcacc agcgattact ttttgctggc ggtaattaaa cttgccgccc tggtcgttgc
    12001 tgccgccagt ggctttcgcg gtgggcgaat cttcccggca gtgtttgtcg gcgtggcatt
    12061 agggttgatg ctgcatgagc acgttcccgc cgtaccagcg gcaataaccg tttcctgcgc
    12121 tattctcggc atcgtgctgg tggtaacacg cgatggctgg ttaagtcttt ttatggcggc
    12181 agtcgttgta cccaatacca cattgctacc gctgctctgt atcgtcatgc ttccggcatg
    12241 gctgttatta gcaggtaagc cgatgatgat ggtcaatcgt ccgaagcaac agccacccca
    12301 cgataacgtt tagcaaaaaa tgctctcctt tatgatttaa gagttatggc gatgatacgg
    12361 ggctttacct ccccgtaata ttgcgttaac aggccgctga cggcttcgat cgtgaaggag
    12421 aataacaatg ttcaggtcac tgtttctggc ggccgccctg atggcattta ccccgcttgc
    12481 agcaaacgca ggcgaaatca ccctactgcc atcaatcaaa ttacaaattg gcgatcgcga
    12541 tcattacggt aattactggg acggtggtca ctggcgcgac cgtgactact ggcatcgcaa
    12601 ttatgaatgg cgtaaaaacc gctggtggcg tcatgataat ggctaccatc gtggctggga
    12661 taagcgtaaa gcatatgagc gtggctatcg tgaaggctgg cgcgatcgtg acgatcatcg
    12721 cggaaaaggc cgcggacatg gacaccgcca ttaaacacat tcgccaatgg agcacaaggc
    12781 ctgatgcgac gctagtgcgt cttatcaggc ctacaaaccg gctctttaac gcgtagggcg
    12841 gataaggcgt ttacgccgca tcgccaatag tgccagatgc gacgctcatt caactacaat
    12901 cccagcgccg tccccaccaa taaccagata ttcagcgcca cgaccagcac tacaatcacc
    12961 cagcctgtct gttttacgcg tttgctgttc accagatcgc ccatcaactt gctgtcactg
    13021 gtgaaaatca gcagtggaac cagcgccagg gcgataccaa aacttaacag cacctgactc
    13081 ataaccagaa tccgtgtcgg atctaatccc atcagaatga caataaatga cggcagcatg
    13141 gtgactgtac gacgcaccca cagcgggata tggaagcgaa tgaacccctg catcaccacc
    13201 tgccctgcca gtgtccccac caccgttgag gacagtccgg cagcaaccag acttaaccca
    13261 aagaccgttg ccgcagcatg gcttaacagc ggttgcagcg tcaaataagc ctcatcaaga
    13321 tcggcaacac cagtatgacc agaaaagtgg aacgccgccg cagctgtagc catcatcgcc
    13381 agattgacaa aaccggcaat agtcatggcg atagccacat cccatttggt ggcggaataa
    13441 cgttgttgac gcgaaccgcc atgtaaatgc tgagtgagcg aagagtgcaa ataaatcaca
    13501 tgcggcataa tcgtcgcccc taacacgcct gctgccagga agaccgcctc cgaagtaggt
    13561 aaactcggga tcaccattcc tttacccagc tgcgccaggt taggctggga gaaaatcaac
    13621 tcgacaatgt aagccgcggc aacaaacaac agtaacccgc caatcacttt ctccagcggt
    13681 ttttgcccgc gacgttgcag cattaaaatc aggaaagtcg cgatccccgt cagcaccgcg
    13741 ccctgcaaca acgaaacacc aagaatgagt ttaaaaccga tcgccgcacc aataaattcc
    13801 gccaggtcgg ttgccatcgc aataatttct gcctgaaccc aatagaacca cactacggga
    13861 cgcggatagt gatcgcgaat ctgctccgcc agatttttac cggtggcaat ccctagtttg
    13921 gcagagagga tctgaatcag catcgccatc aggttggccc aaacgacaac ccacagtagc
    13981 tgatagccga agctggcacc cgcctgaata ttggtcgcaa agttaccggg atcgatataa
    14041 ccaatcgccg caatgaacgc aggtcccatt aatgcgagcc tcatcttgcg cgccgcccgt
    14101 ccgctgctac tctcaacgcg atagttcgtc atcttgtgcc tctaaaacat agcctttgct
    14161 atgtttcatg ctatgccaaa cgagaatgat tatcaaattc atttaaatgg gttgtggtga
    14221 tttctctgat agaccaggat tatgactacg aaaagattga tgacttgaat gttatggtta
    14281 tgtttaatgt tagcacattt acataacttt cagcttccat acacaacata gcagaaatgt
    14341 atgacagctc actatttttg aagcttgtca caggacgtca ttatagtgtg tgtcagatct
    14401 cgttttcctt aaccatgtta catagaatgt gcacggaaat ttaacctgcc tcatatttgg
    14461 agcaaatatg gaccgcgtcc ttcattttgt actggcactt gccgttgttg cgatcctcgc
    14521 actgctggta agcagcgacc gcaaaaaaat tcgtatccgt tatgttattc aactgcttgt
    14581 tatcgaagtg ttactggcgt ggttcttcct gaactccgac gttggtttag gcttcgtgaa
    14641 aggcttctcc gaaatgttcg aaaaactgct cggatttgcc aacgaaggga ctaacttcgt
    14701 ctttggtagc atgaatgatc aaggcctggc attcttcttc ctgaaagtgc tgtgcccaat
    14761 cgtctttatc tctgcactga tcggtattct ccagcacatt cgcgtgttgc cggtgattat
    14821 ccgcgcaatt ggtttcctgc tctccaaagt caacggcatg ggcaaactgg aatcctttaa
    14881 cgccgtcagc tccctgattc tgggtcagtc tgaaaacttt attgcctata aagatatcct
    14941 tggcaaaatc tcccgtaacc gtatgtacac catggcagca acggcgatgt ccaccgtttc
    15001 tatgtccatc gttggtgcat acatgaccat gctggaacca aaatacgtcg ttgcagcgct
    15061 ggtactgaac atgttcagca cctttatcgt gctgtcgctg atcaaccctt accgtgttga
    15121 tgcctgtgaa gaaaacattc agatgtccaa cctgcacgaa ggtcagagct tcttcgaaat
    15181 gctgggtgaa tacattctgg caggtttcaa agttgccatt atcgttgccg caatgctgat
    15241 tggctttatc gccctgatcg ccgcgctgaa cgcactgttt gctaccgtga ctggctggat
    15301 tggctacagc atctccttcc agggcatcct gggctacatc ttctatccga ttgcatgggt
    15361 gatgggtgtt ccttccagtg aagcactgca agtgggcagt atcatggcga ccaaactggt
    15421 ttccaacgag ttcgttgcga tgatggatct tcagaaaatt gcctctacac tctctccgcg
    15481 tgctgaaggc atcatctctg tgttcctggt ttccttcgct aacttctctt caatcgggat
    15541 tatcgcaggt gcagttaaag gcctgaatga agagcaaggt aacgtggttt ctcgcttcgg
    15601 tctgaagctg gtttacggct ctaccctggt gagtgtgctg tctgcgtcaa tcgcagcact
    15661 ggtgctgtaa gaccatacat aaaaaagccg gggataattc cccggctttt ttacgcctgt
    15721 taatcagcta atggctgcgg gcgaccaatc aaataccctt gcagatattg caccccgagc
    15781 ttatgcaata gcgcctgttg ctgttgcgtc tcgacaaact ccgcgaccac actcaatgac
    15841 ttcgctttcg ccagatcggt aattgatctc acaatcatcg catccagcgt attcgtgaca
    15901 atatctttca caaagacgcc atcaattttg atgatatcag cctgcaaacg ctttaaccgt
    15961 tcgtagttgg catagccggt gccaaagtca tcaatcgcaa tccggaaacc aaacttatgc
    16021 agctgctcga tgttgtacat gctgctttct gcgttagaaa acgcctgctc ctcggtgatc
    16081 tcaagaatga ccgcctgcgg ggagatgtga taacgcttaa atagacgaat aatccgcccg
    16141 gcaatattct tttgcagcag cgtgagcggc attaaattaa ctgaaaagcg cgggcctttt
    16201 ttgtcgcaag ggtgtgttgc cagccacttc aacaaggatt ccagcacttg caaatcaaaa
    16261 cgcgcgctaa ggttaaactg agcaataagg ggcagaaact tatccggggt cataatgccg
    16321 ccgtcatatt tcagtcgcgc gaggacctca tcataacctt caccctcttt gttgcgaatt
    16381 ggctgggcgt agagcagcaa atcaccctga tctaacgccg tgcgaatggt attcagcaat
    16441 agcacctgtt tagtggtctg cccggaaacc atctcctctc ggctatccag cgccagcaca
    16501 tgatgatgtg cgcaggattg ctccgccagc cagcttaact gccccaacaa gggttgcagg
    16561 gtttcctgat ttccatcaaa acgcccccag gcagcaccat agcccatatc cagcccggta
    16621 ttgttccagt gaatttgccg actattcagg atgttaacca tatgctggag tcgcccttcc
    16681 gtttcaggcc cgctcagcac taacagcagt tcactacccg gcaattgata caacttttcg
    16741 ttttcctgca tcaatggcag cagcgtacgg taaattgatc ggatacagtg aacacgcatc
    16801 atcaacccgt aatgacgact cataaactca agattatcaa tgcgcaggca gcaaaaactc
    16861 ttgcccgcct cttgctccgg cgcttgttcc aacgcacgaa agttgggcag gagcgttaac
    16921 ggatcggtca gcgcctgcaa atgccagcgg cgattaagcc attcactgcg atgataaatg
    16981 cgcaccatat agagcaggca aacgctaaag gaaatcagca ccgcgagaat aaatgccagc
    17041 gaatattcgg tttccacccc ttgcaaaaag ttctggttgt aattcagcag gcaaagcgtt
    17101 gaaacagccc aagtgagatt taaaaacgga tagcgaagct taccgacccc gagggtaaag
    17161 atgatgaaga aaacgggtac caggtaaccg gcaataaagt cgttttcata aggtgtgcac
    17221 aacagaagta gcagcacgct taacgctgcc agccagctta aggtaaatgc gcgtttctct
    17281 ttgcccaacg acggagcgat atccctgcgc cacaatatct gcgcaaagtg gggacttaca
    17341 atcatgcgag tgagatagta gaaaagcatg ttgtaaatca gcacagcggt gaaaaggctt
    17401 agcaaatcaa cgaccgtgaa aatggcatcc gcatcgccga aaaaggtgga tatctttagc
    17461 gggaaatcaa agaagctccc cacaagatac atgctgcatt tgatgccgat cggcgtcacc
    17521 aaaccaagcc agaacagacg ttgccagaca tatcggctgg tcaatccata acgccaacgc
    17581 gtccccagct gccagcgtaa tattgcgcag gcacacaaca cgacaaacgt ctggctaccg
    17641 agcatgaccg cagtctgtaa aaaagagaga tgaaagttcc acagattggt gcaaaacatc
    17701 ccgcataaaa cgggaacgac accgcgccag ccaaagataa acaagattga cagcattacg
    17761 cacagtggca tccacgccag aaagatataa ctggaatgga taatggcaag tggcgaaata
    17821 aaacgggata gctgaataag taccacggtg agcgtaaacg ctagtgtgaa tatcttgata
    17881 ttttttatca ggttatgctc cacaaacatg agtacactac tctcactgat cggggttttc
    17941 aggcacacaa ataataagtt tattcgcatt gcgaagcaaa caggggccat cctgttgccg
    18001 atttttcatc caatgatgct ttcaccaggc ggcactaacc cggacgtaga ctgtattaca
    18061 aaagcggcaa aaagcagaga caaaaaaccc ccgctttgca gcgagggttg gaaatttggt
    18121 ggagctaagc gggatcgaac cgctgacctc ttgcatgcca tgcaagcgct ctcccagctg
    18181 agctatagcc ccacgatgcg tttacgtacc aaatttgctg ggtgcaaaat ttggtggagc
    18241 taagcgggat cgaaccgctg acctcttgca tgccatgcaa gcgctctccc agctgagcta
    18301 tagccccgtc acgtaaagct tgtcgagttg acgggcggca tcatatgaat tccgcccgaa
    18361 tgtgtcaacg gcaaattgca acgtgtagtt tcaatcgctg aaaaatcagg caaatgaaca
    18421 attttggaaa accgctcgca ttcagtagtt attcatgtca cggtttcctg taaagtggtg
    18481 ctataaaatg aactactaat agacccacat acattcaggg aattgttatg ttcaaggaga
    18541 ggatgacacc agatgaactt gccagactga ccggctatag ccgccagacc attaataaat
    18601 gggtacgcaa ggaaggctgg acaacgtcac caaaacctgg cgtccagggt ggcaaagccc
    18661 gactggttca cgtcaatgaa caggttcgtg aatatattcg taatgctgaa cgtccagaag
    18721 gtcagggaga agcgcctgcc ctttctggtg atgcgccgct tgaagtattg ctggtgacac
    18781 tggcgaaaga gatgacgcca gttgaacaaa aacagtttac atccttgctt ctgcgggaag
    18841 ggattatcgg attgttacaa cgcttaggga ttcgcgatag caaataatat gaaaagatta
    18901 cgcaataaaa tgaccaccga agaactggct gaatgcctcg gtgtggctaa acaaactgtt
    18961 aaccgttgga tcagagaaaa aggctggaaa acggaaaaat ttcccggcgt gaaaggcggt
    19021 cgtgccagac ttattctggt cgatacgcaa gtttgcgagt ttattcagaa cacgccagcc
    19081 ttccataaca caccgatgct tctggaagcc gaagaacccc ttgccgaata cgcaccgggg
    19141 attcgcactc ctgcttatcg gcaaatcatc agtgcaatag acaacatgac tcacagtgag
    19201 caggaaaaag ttgcgcaatt tttgtcacgc gaaggaattc gtaatttcct tgctcgtctc
    19261 gacatagatg aatcagcttg acatcctcca cgccctgaat gacgaggatc cctgctacgt
    19321 tcaggctgtc gcctgaatca tttcggtggg ttcctgcttc aacgagcggc ctgactgcac
    19381 catctctcca caggcaagaa cggcgtgccc cgccgctaaa atgttacgag cgccgtttac
    19441 atcggcgttc gctgtatatc cacatacctg gcatctgaat ttactttgtg acaggcggtt
    19501 ttctttcgct gtatgaccac agcacgcgca acgctggctt gtgtacgctg gcggaacagc
    19561 cagtacctga ccgccacgcc agagctgctt gtactcaagc tggcggcgca tttcatacca
    19621 gccctgatcc agtatcgaac ggtttaaacc tgattttgcc cggacattgc gccccggctg
    19681 gctgaccgta cccgctgctg actttgacat gttgctgacc ttcaaatcct caatgactat
    19741 cattgcgtgg tttttgctga cggtcgttgt gactttgtga aggtagtccc tgcggatatt
    19801 tgcggtacag gaatgcagtc gctgtatttt gcgtttctgc ttctgccagt tattgctgaa
    19861 tttgaccttg cggcttaact gtcgctgaag tctcgccaac ttcttctggt ttttctgaaa
    19921 actgtttaca ggctcaaaga ctgtgccatc tgacagcgtg gcgagtttag ccacgccagc
    19981 atccagtccg accattgatg ctgaagggtg aaccggagtt gatacttcac tttctgtctg
    20041 aatactgatg taccacttac cgcaggactg gctgacagtg acatttttca caacacccgt
    20101 gacctgacgg ctgttcaggt agcgcatcca gccaagtttc ggcagaaaaa tacggctgtt
    20161 ttcctgatcg agcttaacgc cctgcgggta gcggaatgca tcattctgtc cccgcttttt
    20221 gaatcgggga aaagcagccc gcttccggaa gaagtttttg taagcccgct caagctcttt
    20281 cagtgactgt tgcaatggct gtgacggggc atctttaagc cattgcgttt cagtggcgtt
    20341 tttccactca accagccagg aagccatttt cccgtaaggg atgtatttat taccggcctc
    20401 atgattctca ttctgaagcg ccagagcacg attgaaaacg aaacgacatg cgcctgcgaa
    20461 gcgcctcatt tcacgctcct gttgaccacc tggtcttaac tggaatttaa atgcctgtag
    20521 tcgtttcaca ggccaaatga tactttggtc tatgaataaa gaaaccgata tccgacgggg
    20581 aaggcattgt gttttcctga tgcatgttca tctgttcttt gtcaccagat accgacgtca
    20641 gatttttgac tatgacgcga cagaaaaact acgcacttac ttttcaaatg tatgtgctga
    20701 ttttgaagct gaactggttg aaatggatgg cgaaccagat cacgtccatt tgttaactaa
    20761 ctatcctccc aaactggcga tatccagtct ggtaaacagc ctcaaaggcg tatcgggtag
    20821 gttactgcga cgagatcgac cagatattgc agtcaggtat tactacaaag gcgttttgtg
    20881 gagtcctggc tattttgcca gtagctgcgg aggtgcgcca atatccgtca tccgccaata
    20941 cattgaacaa cagcaaacac ctggtcaggt ggaaaaccgc gccttatatc cccgccctga
    21001 aggacggggt tttacggcgc accggataaa taaaaaacgg caggggtatc tctcctgccg
    21061 tttatctttt atgcgctaat tactgctgat tttcgcgttc cgcaataaaa gccagcgctt
    21121 tgttgatacg ctcgatactg cgggtcttac cgatcgcgtg aacggtaacg tccagcgccg
    21181 gagactgccc cgcaccggtt acggcgacac gcagcggcat accaacttta cccataccca
    21241 cttccagctc atccgccgtc gcctgaatag cgtgatgaac gttttcagct gtccagtcag
    21301 taatcgcggc cagtttgtca cgaaccactt ccagcggctg acgcgctacc ggacgcagat
    21361 gttttttcgc ggcgtcggca tcgaactcag caaaatcttc gtagaaataa cggcagctct
    21421 gtgccatctc tttcagcgtc ttgcagcgtt cgcccagcag tttcaccaga tcagccagct
    21481 gcgggccgtt acgggtatcg atattttcct gctcaatgtg ccactgtaag tgagtagcaa
    21541 catactccgg cggcagcgcg ttaatgtagt gatggttcag ccacagcagc ttgtcggtgt
    21601 tgaacgcact ggcagatttg ctgacggcat tcaaagtgaa gtatttgatc atctcttcac
    21661 gagtgaagat ttcctgatcg ccgtgggacc agcccagacg caccagatag ttcagcagtg
    21721 cttctggcaa ataaccgtca tcacgatact gcattacgct gactgccccg tgacgtttgg
    21781 acagtttttt gccgtcatcg ccgttgatca tagaaacgtg cgcgtaaacc ggtaccggcg
    21841 ctttcagggc tttaagaatg ttgatctggc gtggcgtgtt gttgatatgg tcttcgccac
    21901 ggataacgtg ggtgatttcc atatcccagt catcgacaac cacacagaag ttataggttg
    21961 gggaaccatc ggtacggcgg ataataagat cgtccagttc ctggttgctg aactcgatcg
    22021 gaccacggat ctgatcgtca aaaacaacag aaccttcctg cgggttagca aaacgtacga
    22081 cacacggctc atcatcagcg tgatgctcat gactgtggcg gcagcgaccg tcataacgcg
    22141 gcttctcacc tttcgccatt tgctcttcgc gcagcgcttc caggcgctct ttagagcaat
    22201 agcatttata agctgtgccc tcttccagca tctgatcgat caccgcgttg tagcgatcaa
    22261 aacgtttggt ctggaagtac ggaccttcat cccactccag gctcagccag ttcatgccat
    22321 ccataatggc ttcgatagct tccggcgtgg aacgctcaag atcggtgtct tcaatacgca
    22381 gcacgaactc accgccgtga ttacgtgcaa aaagccagga gtaaagagca gtacgcgcgc
    22441 cgccaacgtg cagatagcct gttgggcttg gcgcgaagcg agttttgatt ttcatgaaat
    22501 ggccttacgt ttagaaagat gccgacaacc ggcaaatcct ggaaaaattg agtgggcgat
    22561 attctatcac tcccgcctga ttcctcaatg tagttcgggt tgcaacctta cgcattattg
    22621 ttttcgttga caaattgcgc aacacggtct gttttgcgat catcttgctt aaatttacga
    22681 cgaacgaaca atttctttag aaaatgcgtt gactcatttt gaactctccc tataatgcga
    22741 ctccacacag cgggggtgat tagctcagct gggagagcac ctcccttaca aggagggggt
    22801 cggcggttcg atcccgtcat cacccaccaa ctactttatg tagtctccgc cgtgtagcaa
    22861 gaaattgaga agtgggtgat tagctcagct gggagagcac ctcccttaca aggagggggt
    22921 cggcggttcg atcccgtcat cacccaccac tttctcacca gctaaatttc ttgtaaaaat
    22981 gtgaagtacc gaagtgggtg attagctcag ctgggagagc acctccctta caaggagggg
    23041 gtcggcggtt cgatcccgtc atcacccacc acttcgggtc gttagctcag ttggtagagc
    23101 agttgacttt taatcaattg gtcgcaggtt cgaatcctgc acgacccacc aatgtaaaaa
    23161 agcgccctaa aggcgctttt ttgctatctg cgatttgtaa gattgatttt tcttctcaag
    23221 ttccttgttc ttctgccgat attacctcgt acagtgacga ggactgaaca atggccacta
    23281 tctcttcaac ttcaattcct tccatacaaa cacagagtag taacagggca tcacaaggta
    23341 gcgatgttgc ttcacaaatc gcgcggataa gtcagcaaat catcaaactc actcaacaca
    23401 ttaaagagat tgtagacact agcggcagtg ctgaagacaa gcaaaaacag gccgaattga
    23461 ttcaacagca aatcacccta ctggaaacac aactggcgca gttgcagaga cagcaggcag
    23521 aaaaggcgca ggagaaagag cagcgtttgt ctctgaatgt atcccttcta aatcctgttg
    23581 aaaatacgac tcatatcggt atctatatct gatacaacgg atccccttcc cgcccagcca
    23641 ttagcgtatt gccctggtct cgtaccacgt cccataacgc ttcagcggcg gtggaaagtg
    23701 aacggttttt cctacgcacc agcatcaact ggcgttccac caccggcgta atgcgcttta
    23761 ccaccagcgg gctaccttca ggcaatggta gcgccagtgc gggaagaata ctgatgccga
    23821 tgcctgcggc taccatcggg aacagcgtcg ccggatgtcc aatctcctgc acaatattcg
    23881 cctgaatacc attgcgcgcc agcgctgcgt caatcagcgg tcggctgcct gacgcataat
    23941 cctgcaacac caatttcgct ccctgtaatg cctgccaggg tacataatct tccgcggcca
    24001 aagcgctatc ccgatggcaa agcagaaaga aaggctcgga aagaatcact tcacattgca
    24061 gatcgcccac tgggcctgga tcgatgacga tgccaaagtc gacatctccc tggcgaatac
    24121 tttccatcac ccactgctgc ggacgatcgt gcaggacaaa ctgaatatct ggatagcggc
    24181 gatggctttc agcaatgcat tgcggaataa gatgcgcgga aatggtctgg ctggcagcga
    24241 cccgaacttt tccgctcagt tgttgcccca tacgcccggt atcgcgcaac gtgctgttaa
    24301 gttcatccag cagtcgctca agacgcaaag ccaactgctg ccctgcatct gtaagcacca
    24361 cttcacgcgt ggttctgtcc agcaggcgaa caccagtatg attttccagc tccttcacac
    24421 tgtgacttac tgccgactgg ctcaggccga tacgctctcc tgcacgacta aaacttttct
    24481 cctgcgctac tgtgacgaaa acctttaatt gttttaaaga ataattcatc tgttttcttc
    24541 atgaatggat gcaataaatc aattttattt ctcaaactgg aagaagcaca atagaaccat
    24601 cgatcatctg gagtctttat gaaacttttt cgtatcctcg atcctttcac cttaaccctg
    24661 atcacggtgg tgttgctggc ctctttcttt cctgccagag gcgatttcgt ccccttcttt
    24721 gaaaatctga ccaccgcagc tattgccctg ctgttcttta tgcacggcgc gaagttgtcg
    24781 cgtgaggcga ttattgctgg cggtggtcac tggcgactgc atttgtgggt aatgtgcagc
    24841 accttcgtgc tgtttccgat tctgggtgta ctgtttgcct ggtggaaacc ggtaaatgtc
    24901 gacccgatgc tctactccgg ttttctctac ttgtgcattc tcccggctac cgtgcagtct
    24961 gcaatcgcct tcacgtcaat ggcgggcggt aacgtcgcgg cggcggtttg ttctgcgtca
    25021 gcatccagtc tgctggggat tttcctttca ccattgctgg ttggtctggt gatgaatgtt
    25081 cacggtgcag ggggcagcct tgagcaggtc ggtaaaatta tgctgcaact gctgctgccg
    25141 tttgtgttgg ggcatctttc ccggccgtgg attggtgact gggtgtcgcg caataaaaaa
    25201 tggattgcga aaactgacca gacgtccatt ctgttggtgg tttatacagc gttcagcgaa
    25261 gccgtcgtta atggtatctg gcataaagtt ggctggggat cattgctgtt tatcgtggtg
    25321 gtcagctgcg ttcttctggc tatcgtgatt gtagttaacg tctttatggc acgccgactg
    25381 ggcttcaata aggcagatga aattactatc gtcttttgtg gttcgaaaaa gagtctggca
    25441 aatggcatcc cgatggcaaa cattctgttc cccacatcgg tgatcggtat gatggtgctg
    25501 cccctgatga ttttccatca gatccaattg atggtctgtg cggtgctggc gcgtcgatac
    25561 aaacgccaga ccgaacagtt acaggcgcag caggaaagca gcgccgataa agcttaaagc
    25621 ggacgcttca ggggctggac cagttgcgtc agcccctcgg ttttaatcag cagcgtgatt
    25681 tgcattagct caccgagctt ccctgccggg aactcatctt tgcgggcaaa ccacagcaga
    25741 tactcctccg gcaggtcgat taagcgacgc cctttgtatt tgccaaacgg cattatcgta
    25801 ttggctattt caatcagctg ctctttttcc atctcagcta cccagcaaac gcagcatttc
    25861 cgcttcgtcg atgacttcaa tgcccagttc ctgcgccttc gccagtttag atcctgcagc
    25921 ttcacccgct atcaccagat cggttttctt cgacacgctg cccgcgactt tcgcgcccag
    25981 ttcgaccagt cgagctttag cgtcatcacg cgacatctgg cttaagctgc ccgtaagcac
    26041 cacggtttta ccagcaaacg ggctgtcaat ctcttccgcg ttgataacga tcggcgcagg
    26101 ccagtgaaca ccttccgcca acagctcgct gatgacattg cggttgcttt cttcggcaaa
    26161 gaagttgtga acgtgggatg caacgacaat gccaacatca ggcacctttt gcagctcttc
    26221 aatcgaagcg gcttccagcg cttccagcgt tccgaaatat gccgccagac ctgctgcggt
    26281 ggcctcgccg acttcacgga tgccaagtgc atagaggaag cgagcaaagg tggtttcttt
    26341 cgctttttcc agcgcgttaa ccacgttttg tgccgatttt ggccccatac gctccagtcc
    26401 ggtcagtttg cctgcggtga gtttgaacag atccgccgga gtgtggacat attctttttc
    26461 aaccagctga tcgatgattt tgtcgcccat tccgtcaaca tccatcgcac ggcgggaaac
    26521 aaagtgtttc agcgactctt tacgctgcgc accgcaaatc agaccaccag tacagcgggc
    26581 aaccgcttca ccttccacac gctcaacgtc agaaccacat accggacaat acgtcgggaa
    26641 tacaacctca cgggtatctt ccgggcgttc agaaagcacg acgttaacca cctgcgggat
    26701 cacgtcgcca gcgcggcgaa tcaccacttt atcgccaatg cgtaaaccaa gacgttcgat
    26761 ttcatccgca ttgtgtaagg ttgcgttact caccagcacg cctgcaacat ggacaggttc
    26821 cagacgcgca acaggcgtaa tcgcgccagt acgcccaacc tgaaactcga cgtcacgcac
    26881 aaaggtcatc tgttcctgcg ccgggaattt aaacgctacc gcccagcgcg gggctcgcgc
    26941 gacaaagcca agctgctcct gctgttccag tgagttgacc ttaatcacca cgccgtcgat
    27001 atcaaagccc agcgtcgggc ggtcttcttc cactttgtga tagaacgcca gcacttcttc
    27061 cgccgattca caaagcgtta cccgatcgct gaccggcaac ccccactttt taaattgcag
    27121 taaacggcca agatgagtat ccggcagctc gccaccttcc agaacaccaa cgccatagca
    27181 gaaaaaagtg agcggtcgct tcgctgtaat acgcggatca agctgacgca gtgaaccagc
    27241 tgccgcatta cgtgggttag caaacacttt cccgcccgtg cgtcgcgcat cttcgttaat
    27301 cttttcgaac cccgcctgcg gcaggaacac ttcaccacgc acttccagac gcgccgggat
    27361 attctctccg tgcaacttca gtggaatggc gcgaatagta cgcacattag acgtgatatc
    27421 ttccccggtg gtgccatcgc cacgagtcgc ggcactgact aaaacgccat tttcatacag
    27481 aatactgacg gcaaggccat ccagcttcag ctcacagcac caggtgactt tctcgttgct
    27541 tttcagacgg tcctgcacac gtttgttgaa ggcaagaaag ctttcttcat caaaaacgtt
    27601 atccagcgac agcattggca cttcatggcg aatctggctg aaagccgcca gcggcgcagc
    27661 acctacacgt tgggtaggcg aatcaggcgt aatcagttct ggatgtttgg tttccagctc
    27721 gcgcagttcg cgcatcagcc tgtcgtattc agcgtcggga atttccggcg catccatcac
    27781 atgataaaga tattcatgat ggcgaagcgt cgttcgcagt tctgtcagtt gttgttcgat
    27841 tgattccata tcgcaccatc aatgctaaaa acccccgaca agcgggggtt cgaagaggag
    27901 ttaatttgcc ttaagtgtat caggcgttgg cgtctttaac ttcgcggatg atgtcctggt
    27961 actcgcgcaa tttctgcgga gtcatcatac ggcgctgatc gtcaagcacg acaccgccca
    28021 cttcatcggc aatatgctgc gcagattgca gcatcagctt gaagttctgc agctcgtcac
    28081 cgtaagacgg tacctgcata aagatagtga cacccggagt agtgaaatcc ttcatttcag
    28141 gatcaaaggt tcccggtttc accatattcg ccaggctaaa taacgccggg ccgctgccat
    28201 ccgggctaag atgacgatgg taaatattca tatcgccaaa aatgaagccc gcttgttgaa
    28261 tgctgttaag aagcagttca ccgtttagct cgctaccgtg atgcgccgcg acgttcatga
    28321 taatcaccgc ttctttgcgc ttcggtttat ccataactgg agccggttcc gctacaggct
    28381 caggctgtgg tgccgctacg ggttctgcag gctggaaagc ctgttgtgcc ggttgcggtg
    28441 ctgaatgcac tggctgcggc gctggcgcga cctgtggcga aactggctgc tgcaacggct
    28501 gttcaggctg cggctgatag gcaggctgct gcacgggttg ctgcaccggc tgcgctggac
    28561 gcggagcatg ttgcggcggt acctgcgctt caggcggctg ctggaccggt tgacgcggct
    28621 gcgcagacgc ataaggcggt tggtactggt gttgcggcga cggacgagca gcctcatgct
    28681 cctgggcgtt agccggggca tgattcacgc ggtgaacacg aacctcacca acgccctcat
    28741 catcttcgac atcctcgtca taagaatcgt cgtcacgttt tgacttcatt cgttttaatg
    28801 gccgatcgcg gaacatagaa gatcgttctt tacggctggt ccagaaacca tgtaccagta
    28861 aagcgattat ggcgatcgcg ccaacaatga ttaatatcag acgcaaatcc tgcatcatta
    28921 tattctctgt tgttctaaca ccttgccacc acggcaaaca tttactcact aagagtattt
    28981 gccgattacc tcaagtgcaa gtgcactatt aactttcaca gcacaaagat agatgaaatc
    29041 gtgctttttg ctgttttttc gaacatatcc taactgtcca ttgcgcaatt acccggtaaa
    29101 atacgcacaa ttttcctggg attggtcaaa aggagctcat cctgattatg gtttcatcat
    29161 tcacatctgc cccacgcagc ggtttttact attttgcgca aggctggaag ctcgtctcgc
    29221 aacctgggat tcggcgtttc gttattttac cgctgctggt caatattttg ttgatggggg
    29281 gcgcattctg gtggctcttt acgcaactcg atgtctggat cccgactctc atgagttatg
    29341 ttccggactg gttacaatgg ctgagctatc tgttgtggcc tctggctgtc atctctgtgc
    29401 tgttagtgtt tggctatttc ttctccacga ttgctaactg gattgccgct ccgtttaacg
    29461 gtttattggc tgaacaactg gaagcacgat tgactggcgc tacaccgcca gataccggga
    29521 ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg cgaatggcaa aaatttgcct
    29581 ggtatctgcc acgcgcaatt gtattgctaa ttctttactt catccctggt attgggcaaa
    29641 ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat gttagccatc cagtattgcg
    29701 attacccctt cgataaccac aaagtgccgt ttaaagagat gcgcaccgcc ctgcgcacac
    29761 gcaaaatcac caatatgcag tttggtgctt taaccagtct gtttacgatg atcccgctgc
    29821 ttaatctgtt cattatgccc gttgccgttt gtggcgcgac ggcgatgtgg gtcgattgct
    29881 atcgcgataa acacgcgatg tggcggtaac aatctaccgg ttattttgta aaccgtttgt
    29941 gtgaaacagg gggggcttat gccgcccctt attccatctt gcatgtcatt atttcccttc
    30001 tgcatataga tatgctaaat ccttacttcc gcatattctc tgagcgggta tgctacctgt
    30061 tgtatcccaa tttcatacag ttaaggacag gccatgagta agatttttga agataactcg
    30121 ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca tcggtaacgg acgcattctg
    30181 gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt gccgtatcgg tgccaacatg
    30241 atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg ttgaactggt tgaaccaacc
    30301 agcggtaata ccgggattgc actggcctat gtagctgccg ctcgcggtta caaactcacc
    30361 ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc tgctgaaagc gttaggtgca
    30421 aacctggtgc tgaccgaagg tgctaaaggc atgaaaggcg caatccaaaa agcagaagaa
    30481 attgtcgcca gcaatccaga gaaatacctg ctgctgcaac aattcagcaa tccggcaaac
    30541 cctgaaattc acgaaaagac caccggtccg gagatctggg aagataccga cggtcaggtt
    30601 gatgtattta ttgctggcgt tgggactggc ggtacgctga ctggcgtcag ccgctacatt
    30661 aaaggcacca aaggtaagac cgatcttatc tctgtcgccg ttgagccaac cgattctcca
    30721 gttatcgccc aggcgctggc aggtgaagag attaaacctg gcccgcataa aattcagggt
    30781 attggcgctg gttttatccc ggctaacctc gatctcaagc tggtcgataa agtcattggc
    30841 atcaccaatg aagaagcgat ttctaccgcg cgtcgtctga tggaagaaga aggtattctt
    30901 gcaggtatct cttctggagc agctgttgct gcggcgttga aactacaaga agatgaaagc
    30961 tttaccaaca agaatattgt ggttattcta ccgtcatcgg gtgagcgtta tttaagcacc
    31021 gcattgtttg ccgatctctt cactgagaaa gaattgcaac agtaatgcca gcttgttaaa
    31081 aatgcgtaaa aaagcacctt ttcaggtgct tttttgtggc ctgcttcaaa ctttcgcccc
    31141 tcctggcatt gattcagcct gtcggaactg gtatttaacc agactaatta ttttgatgcg
    31201 cgaaattaat cgttacagga aaagccaaag ctgaatcgat tttatgattt ggttcaattc
    31261 ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc agcggtcaac acccgccagc
    31321 aatggactgt attgcgctct tcgtgcgtcg cgtctgttaa aaactggcgc taacaataca
    31381 ggctaaagtc gaaccgccag gctagacttt agttccacaa cactaaacct ataagttggg
    31441 gaaatacaat gttccagcaa gaagttacca ttaccgctcc gaacggtctg cacacccgcc
    31501 ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc tgaaattact gtgacttcca
    31561 acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca gactctgggc ctgactcaag
    31621 gtacagttgt gactatctcc gcagaaggcg aagacgagca gaaagcggtt gaacatctgg
    31681 ttaaactgat ggcggaactc gagtaatttc ccgggttctt ttaaaaatca gtcacaagta
    31741 aggtagggtt atgatttcag gcattttagc atccccgggt atcgctttcg gtaaagctct
    31801 gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa atttctgccg accaggttga
    31861 tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca tcagcccagc tggaaacgat
    31921 caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa gccatctttg aagggcatat
    31981 tatgctgctc gaagatgagg agctggagca ggaaatcata gccctgatta aagataaaca
    32041 catgacagct gacgcagctg ctcatgaagt tatcgaaggt caggcttctg ccctggaaga
    32101 gctggatgat gaatacctga aagaacgtgc ggctgacgta cgtgatatcg gtaagcgcct
    32161 gctgcgcaac atcctgggcc tgaagattat cgacctgagc gccattcagg atgaagtcat
    32221 tctggttgcc gctgacctga caccgtccga aaccgcacag ctgaacctga agaaggtgct
    32281 gggtttcatc accgacgcgg gtggccgtac ttcccacacc tctatcatgg cgcgttctct
    32341 ggaactgcct gctatcgtgg gtaccggtag cgtcacctct caggtgaaaa atgacgacta
    32401 tctgattctg gatgccgtaa ataatcaggt ttacgtcaat ccaaccaacg aagttattga
    32461 taaaatgcgc gctgttcagg agcaagtggc ttctgaaaaa gcagagcttg ctaaactgaa
    32521 agatctgcca gctattacgc tggacggtca ccaagtagaa gtatgcgcta acattggtac
    32581 ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa ggcgttggtc tgtatcgtac
    32641 tgagttcctg ttcatggacc gcgacgcgct gcccactgaa gaagaacagt ttgctgctta
    32701 caaagcagtg gctgaagcgt gtggctcgca ggcggttatc gttcgtacca tggacatcgg
    32761 cggcgacaaa gagctgccat acatgaactt cccgaaagaa gagaacccgt tcctcggctg
    32821 gcgcgctatc cgtatcgcaa tggatcgtaa agagatcctg cgcgatcagc tccgcgctat
    32881 cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc ccgatgatca tctctgttga
    32941 agaagtgcgt gcactgcgca aagagatcga aatctacaaa caggaactgc gcgacgaagg
    33001 taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg gaaacaccgg ctgccgcaac
    33061 aattgcacgt catttagcca aagaagttga tttctttagt atcggcacca atgatttaac
    33121 gcagtacact ctggcagttg accgtggtaa tgatatgatt tcacaccttt accagccaat
    33181 gtcaccgtcc gtgctgaact tgatcaagca agttattgat gcttctcatg ctgaaggcaa
    33241 atggactggc atgtgtggtg agcttgctgg cgatgaacgt gctacacttc tgttgctggg
    33301 gatgggtctg gacgaattct ctatgagcgc catttctatc ccgcgcatta agaagattat
    33361 ccgtaacacg aacttcgaag atgcgaaggt gttagcagag caggctcttg ctcaaccgac
    33421 aacggacgag ttaatgacgc tggttaacaa gttcattgaa gaaaaaacaa tctgctaatc
    33481 cacgagatgc ggcccaattt actgcttagg agaagatcat gggtttgttc gataaactga
    33541 aatctctggt ttccgacgac aagaaggata ccggaactat tgagatcatt gctccgctct
    33601 ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt ttttgcggaa aaaatcgttg
    33661 gtgatggtat tgctatcaaa ccaacgggta acaaaatggt cgcgcctgta gacggcacca
    33721 ttggtaaaat ctttgaaacc aaccacgcat tctctatcga atctgatagc ggcgttgaac
    33781 tgttcgtcca cttcggtatc gacaccgttg aactgaaagg cgaaggcttc aagcgtattg
    33841 ctgaagaagg tcagcgcgtg aaagttggcg atactgtcat tgaatttgat ctgccgctgc
    33901 tggaagagaa agccaagtct accctgactc cggttgttat ctccaacatg gacgaaatca
    33961 aagaactgat caaactgtcc ggtagcgtga ctgtgggtga aaccccggtt atccgcatca
    34021 agaagtaatt cttgctgcag tgaaaaatgg cgcccatcgg cgccattttt ttatgcttcc
    34081 gccagcggcg gcaaaatcaa ttcatcgctc tcatgctgct gggtgtagcg catcacttcc
    34141 agtacgcgca accccgcgcg gtgcaccgca tcgtttaatg ccttcccttt cagcaaacca
    34201 ctgataagct gagcacaaaa caggtcgcca gtccctttca ggtcggtttt tacccttgaa
    34261 tgggaaatga cattcacgct gtcggcagtg accaccacaa ccagcatctc ctgattttct
    34321 tcattaccgg aggcgctggt aatcaccacc cattttagtg tgtctgaaag cagacttttt
    34381 gcggcagcaa tggcactgtc gagatcgcgg caatttttac cggtcaggat ttccaactca
    34441 aagatattgg gggtaattcc ctgcgccagc ggcagtaaat attgtcgata cgcttcggga
    34501 aggtcaggtt tgacataaat tccgctgtca atatcgccaa tcaccggatc gaccatgatc
    34561 aataggtcag gatggtcttt gcgtagcgca gtcagccact cggcaaggat tttgatttgc
    34621 gatgccgttc ccatatagcc cgtggttaca gcacgaagtt ggcgcagcgc atcacgctcc
    34681 tgaagtgcac gcaaatagcc gctaaaccat tcatccggaa tcgcaccacc gtagaaagtg
    34741 tcataatgcg gcgtattgct cagcaatacc gtcggcacgg caaagacatt caggccgttc
    34801 tgtttgatag caggcacggc aatgctgttg cccacgctgc cgtaaaccac ctgcgactgc
    34861 acggcgacga tatccgcctg cagcgccctg ctcttatcgt taaacaacaa caaactactc
    34921 atttaatttt ttctccttgc cgatgatcct catcgtattc caaccgaaac tttacctgat
    34981 tctggcagtc aaatcggcta ttacaaaaca aggataaggt aattcagtga agaaaatcat
    35041 ttgtctggtc attacactac taatgacact ccccgcttac gcgaagttaa ctgcccatga
    35101 agaagcccgc atcaacgcca tgctggaggg attagcacag aaaaaggatt tgatatttgt
    35161 gcgcaacggt gatgaacata cctgcgatga agcggtttct catctgcgtc tgaagctggg
    35221 caatacccgt aaccgcattg acactgccga gcagtttatt gataaggttg cttcgtcgtc
    35281 atcgattacc gggaagccgt atattgtgaa gatccccggt aagagcgatg agaatgcaca
    35341 gcctttttac atgcgttaat tgcgcagacg gataaaacgg tgcctgcgca ataatcttaa
    35401 atccccgccc cctgactaaa atgctcttcc ccaaacaccc cggtggaaag gtagcgatcg
    35461 ccacgatcgc agatgatcgc taccaccacc gcaccagggt tagcttttgc cacccgcagt
    35521 gctccggcaa ccgcgccgcc ggagctgacg ccacagaata ttccttcccg caccgccagt
    35581 tcgcgcatgg tgttttccgc atcgcgctga tgaatatcca gcacctcatc taccagagaa
    35641 gcgttgaaaa tccccggcag atattccgca ggccagcggc gaatgccggg aatgctactg
    35701 ccctcttccg gctgcaggcc aacaatggtc accggtttgg attgttcgcg cataaagcgt
    35761 gaaacgccgg tgatagtgcc ggttgttccc atgctggaga caaaataggt aatgcgcccg
    35821 ccagtctgct gccagatttc cggcccggta gtggtgtaat gggcgtaagg gttatcggga
    35881 ttattgaact gatcgagcag ctttccttca ccacgatccg ccatctccag cgccagatcg
    35941 cgcgcacctt ccatgccctg ctctttggtg accagaatca gttccgcacc ataagcacgc
    36001 atcgccgcac ggcgttcctg gctcatgttg tcgggcatca gcaatttcat gcgatagcct
    36061 ttaagcgcgg caatcattgc cagcgcaatg ccggtgttac cactggtggc ttcgattaac
    36121 acatcacccg gtttaatttc cccgcgcttt tccgcctcga cgatcatcga aagcgccgca
    36181 cgatctttta ccgaacctgc cgggttattg ccttccagtt ttaaccacac ttcactgccg
    36241 ttattcggcc ccattcgctg caacttcacc agaggcgtat tgcctattgt ttgttctaat
    36301 gtactcacga tctctatcca tacgtggtgt tgcctgatgc gacgcttgcg cgtcttatca
    36361 ggcctacagg ttacaaacct tgccattaaa aagcccggat cgcgggaagc gcctccgggc
    36421 gtttaacatt cactcaacct atcaggcgct ttgtgcgaga gcaagttcct catctcgggg
    36481 ttcgatacgc tcgtcgccgt tatacagccg cgcatgttgc agaccaacga ataaacgctc
    36541 gccacgctgc ggggcatcgt cgccatgcat cacgaccgtc agcggttcgt tgtaccaccc
    36601 cagcggctgc accactaatt gggtgtagtg acctttcggg ctggcttcca gtacctgtac
    36661 cggcagcggc gaatcgaggc tggtacggcg gctgatatcc acttcccacg ggcgcaggaa
    36721 gagatccacc ggcccctgat acgcaggtgt gtagcccagc ggccagcgat gtgcgccaac
    36781 atggaactgc ccgccgcgaa tggttccctg caggcggttc acttcgccca taaattcgag
    36841 cacaaaacgg gtcgccggtt cgcgccatac ctgatccggc gcgtcagcct gttcaatatt
    36901 gccctggctc atcacaacta cacgatcagc tacttcggtc gcttcttcct gatcgtgggt
    36961 cacaaaaacg ctggtgaatt ttagttcttc atggagttga cgcagccatc gacgcagctc
    37021 tttacgcacc tgcgcatcca gcgcgccaaa cggttcatca agcagcagaa tttgcggttc
    37081 tacagcaagc gcacgcgcca gcgctacacg ctgtttctgg ccgccggaaa gctgcgccgg
    37141 ataacgatcc gccagatggg caagctggac catttccagc aattttgtca ctttcgcttt
    37201 gatggctgcg gcattcgggc gctcgcgacg cggcagcacc gtcaggccaa aagcgatatt
    37261 gtcgaacacc gtcatatggc ggaacagcgc gtaatgctgg aacacgaaac cgactttacg
    37321 atcacgtgcg tgcaggcggc tcacgtcggt gccgtggaag cgaatatgcc cgctggtttg
    37381 atgctccagc ccagcgataa tgcgcagcag cgtggttttc ccggaaccgg acggccccag
    37441 caacgcgacc atctgacctg aaggaatatc cagtgagata tcgttcagca cctgggtgcg
    37501 accaaacgac ttcttaatat tggcaatctc aatgctcatg attttcctcc tgctgcgcgc
    37561 gtttttcctg attctccagg cgccactgca acatactctt taaaaacagg gtgataatcg
    37621 ccatcagcgt caacagcgcg gcggcggtaa aggagccgac tgtgttgtag tcctgctcca
    37681 gcaattcaat ctgtaacggc agcgaaagtg tttcgccacg aatcgagccg gaaaccaccg
    37741 acaccgcgcc aaactcgcca atcgcgcggg cgttggtcag cactacgcca taaagcagcg
    37801 cccagcggat gttcggcaat gtgacacggc ggaacatctg ccagccggat gcgccaagca
    37861 aaatcgccgc ttcgtcttcc tgactgccct ggcttaacat caccggcacc agttcgcgca
    37921 ccacaaacgg acacgtcacg aagatcgtga ccagcaccat tccgggccag gagaacataa
    37981 tttgcagatt atgttcatcc agccagccgc cgagcgggcc gttggagccg tagaacagca
    38041 gatacaccag accggcaacc accggtgaca cggcaaaagg aatgtccagt agcgtcagca
    38101 gtaactggcg tccggggaag ttaaagcgcg tcaccagcca ggccagcaga ataccgaaca
    38161 ccaggtttac cggtacggca atcagcgcga tcatcaccgt cagccagatg gcgtgcagca
    38221 tatccggatc ggccagattc tgtaaaaccg gcatcagccc tttgctgaac gcctgtacga
    38281 agatataaat catcggcacc agcaggatga acgccgatac cagcatcccg atgccaatca
    38341 gaaaccattt gccccagtta atcgggcgcg cgtcataacg cttcaattgt gtcacttccg
    38401 ccattaatga cctaccacac gccgaccaaa gcgactttgc agagtgttaa ttgagaacag
    38461 cagcagcaga gatgccgcga ggatcaccga agcaatcgcg ctcgccgccg ggtaatcaaa
    38521 ctcctgtaag cgaacgaaaa tcatcagcga cgtcacttcc gtcttccaag cgatgttccc
    38581 ggcgataaat atcaccgcgc caaactcacc aagactacgg gtaaacgaca acgccacgcc
    38641 cgccaccagc gccggagaaa gttccggcag caccactttg cagaaactct gccagcgcgt
    38701 tgcgccaagc gtttctgccg cttcttcata ttccgggcct aactcttcca gcaccggctg
    38761 cacggtacgt accacaaacg gaatgctggt aaaggccatt gccaccgcaa tccccagcca
    38821 ggtataggtg actttgatat caaacttcgc cagccattca ccgtaaaaac cgtttacaga
    38881 aaagagcgag gccagcgtta aaccggcgac tgccgttggc agcgcaaagg gtaaatccat
    38941 cagcgcatca agcagcgtgc ggcccggaaa gcgataacgg gttaaaatcc acgccatcag
    39001 caggccgaaa acgccgttaa aaattgatgc cacaaacgcc gacagcagcg tcactttgta
    39061 ggccgcgacc acctgcgggt tggtgatcac ttcccagtac tgcgcccagc tcatctcaga
    39121 aagctgcatc accagcgcgg agagtggcag cagcaatatc aggcacacaa acagcagact
    39181 ggtgccgagg cttaaggtaa agcccggcag gacgcgtctt gatgaaactg caaacattaa
    39241 ttacgccccg ccgctaacag cttgtctaac tcgccgccgc tggtaaagtg ggttttcatc
    39301 acttccggcc aggagccaaa tttgtcttcc acgcggaaca gttcggtctg cgggaattta
    39361 tctttcagtt tgtccatgac ttccgggtta ttcacgcggt agtaatagtc ggtgatgatg
    39421 gtttgcgcct gcgggctgta aagccagttc agataggctt ttgctgcttt ttcggtaccg
    39481 ttggcttgca cgtttttatc gacccacgcc accgggaatt ctgccagaat gttggttttc
    39541 ggaatcacca cttcaaagcc ctgcgcttcg tactgtttgc ggatgttgtt cacttccgat
    39601 tcaaagctaa tcagtacatc gcccaggccg cgctcggcaa aggtggtggt cgcgccacga
    39661 ccgccggtat cgaacacttc aacgtttttc aggaactgag tcataaactg ttcggttttg
    39721 gctttgtcgc caccgtcagc tttatccgct gcgccccatg ccgccagata ggtataacgc
    39781 gcgttacccg acgttttcgg gttcgggaaa atcagcttca cgtcggagcg caccaggtcg
    39841 ttccagtcgt ggatattctt cgggttgccc ttacggacca ggaagcccat ggtggagtag
    39901 aacggcgagc tgttattcgg caggcgcgac tgccagtccg ccgggataag cttgccttta
    39961 tcgtgcagaa tttgtacgtc ggtcacctgg ttataagtga caacgtcggc ttttaagccc
    40021 tgcaaaattg ccagcgcctg ttttgatgac ccggcatgag attgttttat cgtcagtttg
    40081 tcgccgccgt tatctttggc ccattgctgt tcaaacggcg ggttcagggc ggcaaacagc
    40141 tcgcgggaga cgtcataaga actgttcagc agttccgttg cctgtacatg gcccgccagc
    40201 agcagagaag cgaccagcgc gagtgagttc tttttcagta agttaacggc cattgcgcac
    40261 ccttataaat ttaatgactt tctaatagcc atcatattta taacggacgt gaaaggagta
    40321 acggttttat ataccgtttg gtgatttgga agttgaaaag ggaataagac gaaatcctcc
    40381 ccacaaatgc agggaggaaa cagaggtgaa tcagataccg acgctaaccg tctccggcag
    40441 tgtgctgccg ccatcaatca cattctgtgt accggttaaa tagctggatt catccgatgc
    40501 gaggaaggcc gccagttcgc cgacttccag cggatcggcg aggcgacgca gcgggattgc
    40561 ttttgccatt tcagtcagca ccgactctgg atcttccggg ttcgactggc gggcaatgct
    40621 ttccgccatt ggtgtgcgaa cgtatccagg gcatatggcg ttaacgcgaa taccagactg
    40681 cgcatactcc accgccagcg attttgtcag gccaacaatc gccgctttcg ttaaggcgta
    40741 cgccgtttca cccggatcgg ccaccatatc accagtgact gaagacatca tcacaatgcg
    40801 accatctttg cgggcaatca tctccggcag caccgccttc gtgacgttcc atacgccttt
    40861 aatattgatg tcaatatgga aatcgcgatc gtcatcgctc atatcgagga aactgcccag
    40921 acgacaaacg cctgcgttat tcaccaggat atcaatgcgc ccttcttttt ccttcgcgcg
    40981 tttgatagct gcggctaccg acgccgggtc acgcacatcg gcgacaaccg ccgtacagcg
    41041 atgaccacga ccacacagtt cgtccgccag cttttcgatc tcaggggaga tatccagcaa
    41101 gattaggttt gcgccatgac gcgcaaaagt tctggcaatt ccttcgccaa ttccctgcaa
    41161 tgcgcccgta atcagtgctg tcttgcccgt gagtttaccc attttaatgc tctccttgtt
    41221 aatacagagc gttacactcc attccctctg aaaatacagg ataaacaaaa tattactgcg
    41281 gatcagctca ctaattcagg aaaatctcaa tccagtcgct gctttatgca tcatttcagg
    41341 cgttgtgtta gctcactgct gcgctgaatc attttcaatg actccacatc attgagttgc
    41401 acaagaccaa caaacagtaa atcggtaaca gaattttgcg ccgtgcgggt agacatcgac
    41461 gagctacgcc attcggtttc accggaaacg gtatcgagcg tgaaatgcgc cagccgccgc
    41521 agcggagagt cagccagaga agtaatggca atcaccgttg ccccctgctt tcgtgccgct
    41581 tctgcgcaca agacaatctc ttttttactg ccgctgtaag agatagcgat ttgtacatcg
    41641 ccttttttca acgcctggga aacggtggct tgtacgtgcg tgtcggcttc gcaggccacg
    41701 cgatatccaa ttttcatcag tttgaacgac agatcgcacc ccaccagcgc cgatccgccc
    41761 aggcccgtta tctggataaa cggtgcttta ctgatgacct caatgatttt ttgcagccgc
    41821 gcgtagtcga acaacgcaca ggtttgctcc agcgccagct ctttttcgcg attcaatttg
    41881 cgggcaatca cttccagcga atcatcactg gtaatcgaac tgtgcaggtg ctgcgccgtg
    41941 gcattcgttt tctcccggct ggcactgtat tcgcctatta acgccatacg caattcagta
    42001 aacccttgtg cgccgagctt ctgggcaaac ttcacaatac tcgactggct gatgccgagc
    42061 tgtttcgcca tcttgcgcga agagacggat ttcagctcac tcacgttggc ccgcagaaag
    42121 tcagcaattt tttgctcgtt ctcggtaaat tcactttccg cattgcgtat tttcgtcagg
    42181 tacaacatct tccactctcc tgggctggca gcaaaaattc cgcttaagtt cttatgacgc
    42241 tcttcacact ctgcgagtgt agactcaatg attcctttaa gtctgtcaat tcgggatata
    42301 tgattccata catctgcaat tatttgatcg taaatagtaa ggtcaccacc gatgcaactt
    42361 gaaaagatga ttaccgaagg ctcgaacgcc gcctcggctg aaattgaccg cgtatcaacg
    42421 ctggaaatgt gccggattat caacgatgaa gataaaaccg taccgcttgc cgttgagcgc
    42481 gtactgccgg atatcgccgc ggcaatcgat gttatccacg cccaggtaag cggcggcggt
    42541 cgtctgattt atctcggtgc gggaacatcc ggtcgtctgg ggattctgga tgccagcgaa
    42601 tgtccgccca cctacggcgt gaaaccgggt ctggtagttg gattaattgc tggcggcgaa
    42661 tatgccattc agcacgcggt ggaaggcgct gaagatagcc gggaaggcgg tgttaatgat
    42721 ttgaaaaaca tcggattaac cgcgcaggat gtggtggttg gcattgctgc cagcggtcgc
    42781 acgccgtatg tgattgccgg actggaatat gcacgccagc tcggctgccg tacagtggga
    42841 atttcctgta atccggggag cgccgtttcc accaccgctg agtttgctat tacgccggtg
    42901 gttggggctg aagtggtcac cggttcttcg cggatgaaag caggcacggc gcagaaactg
    42961 gtgctcaata tgctttccac cgggctgatg attaaatccg gcaaagtgtt cggcaacctg
    43021 atggtcgatg tggtcgccac caacgaaaaa ctgcatgtgc gccaggtcaa tatcgttaaa
    43081 aacgccaccg gatgtaatgc cgaacaagca gaagcagcgt taattgcctg cgagcgcaac
    43141 tgtaaaacgg ccattgtgat ggtgctgaaa aatctcgatg ccgcagaagc taaaaaacgc
    43201 ctggatcaac acggcggctt tattcgtcag gttttagaca aggaataacc catggccaaa
    43261 gagatcagca gtgaacttct gaacaccatt cttacccgtg tcggcggacc gggaaatatc
    43321 gccagttgtg gtaactgtat gacgcgcctg cgtctgggtg tacatgacag ttcactggtt
    43381 gatcccgata tcaaaacgct ggaaggcgtg aagggcgtca ttttgaccag tgaccaggtg
    43441 caggtcgttt ttgggcctgg taaagcgcat cgtgccgcaa aagcgatgag cgagctgctg
    43501 ggagaagcac cggtacagga tgccgcagaa atcgccgccc agaacaaacg tcagttaaaa
    43561 gccaaacaaa cctccggcgt gcaacaattt ctcgccaaat tcgccaccat cttcacgccg
    43621 cttatccccg gttttattgc cgccggtctg ctgctgggga tagcgacgtt aattgccacg
    43681 gtgatgcacg ttccggcaga tgctcagggc acgctacccg acgcgctgaa ttttatgaag
    43741 gtgttcagca aaggtttgtt caccttcctg gtgattctgg tgggctataa cgccgcccag
    43801 gcatttggcg gcacgggcgt caatggcgca attattgccg cactcttttt gctcggttat
    43861 aaccccgctg caaccaccgg ttactacgcc ggttttcacg atttctttgg tctgcccatc
    43921 gatccgcgcg gcaatattat cggcgtgttg attgccgcgt gggcctgcgc ccgcatcgaa
    43981 ggcatggtgc gccgctttat gcccgacgat ctcgacatgc tgctgacctc gttaatcacc
    44041 ctactgatca ccgctacgct ggcctatctg atcattatgc cgctgggcgg ctggttattc
    44101 gaaggtatgt cgtggctgtt tatgcacctg aacagtaatc cgctcggttg tgcggtttta
    44161 gccgggctgt tcctgatcgc cgtggtattt ggcgtgcatc agggctttat tcctgtttac
    44221 ctcgcgttaa tggacagcca aggattcaac agcttatttc ccatcctttc gatggcaggc
    44281 gcgggccagg tgggcgcggc gctggcactc tactggcggg cgcaaccgca cagtgcatta
    44341 cgcagtcagg tacgcggggc gattattccc ggcctgctgg gcgttggcga accgttgatt
    44401 tatggcgtca ccctgccccg catgaaaccg tttattaccg cctgtttagg cggcgcggcg
    44461 ggcggtttgt ttatcggatt gatagcctgg tggggtctgc cgatgggctt aaacagcgcc
    44521 tttgggccgt ctggtctggt ggcgctgccg ctgatgactt cagcacaagg cattcttccg
    44581 gcgatggcag tctacgctgg cgggattctg gtggcatggg tttgcgggtt tattttcacc
    44641 acgctttttg gctgccgtaa cgtcaatctg gactgatatc atgaaacgga caatgctcta
    44701 tctttctctg ctggctgtta gttgtagcgt cagcgccgca aaataccctg ttctgacaga
    44761 aagttcgcca gagaaagcag ggttcaacgt cgaacggctt aaccagatgg atcgctggat
    44821 tagccagcaa gtggacgccg gttatcccgg cgtaaacctg ctgattataa aagataatca
    44881 gattgtgtat cgcaaagcct ggggagcggc gaaaaagtac gatggcagcg tattgatggc
    44941 gcagcctgta aaagctacca ccgggacgct gtatgacctg gcctcaaaca ccaaaatgta
    45001 tgccactaac ttcgctctgc aaaagctaat gagcgaaggc aaactgcatc ctgacgatct
    45061 gattgcgaaa tatatttcgg ggtttgccga tagtccaaat gacaccatca aaggcaaaaa
    45121 cacgctgcgg atttctgacc tgctgcatca cagcggcggt ttcccggcgg acccacaata
    45181 tccgaataaa gcggtcgcgg gcgcgttata ttcccaggat aaaggccaga cgctggagat
    45241 gatcaagcgc acgccgctag agtatcagcc cggcagcaag catatttaca gtgatgtcga
    45301 ttatatgctg cttggattta tcgtcgagtc ggtcaccggt cagccgcttg accgctatgt
    45361 tgaagagtcg atttatcgcc cgctcggcct gacacatacg gtatttaacc cgctgctgaa
    45421 aggctttaaa ccgcaacaaa ttgccgccac ggaattgaac ggcaataccc gcgatggagt
    45481 gatccacttc ccgaatatcc gcacctccac tcactggggt caggtgcacg atgaaaaagc
    45541 cttttattcg atgggcggcg tttccgggca cgcaggtttg ttttccaata ccggcgatat
    45601 tgcggtgtta atgcaaacga tgctgaacgg cgggggctat ggcgatgtgc agctgttcag
    45661 tgcggaaacg gtgaagatgt tcaccaccag ctctaaggaa gatgccactt ttggcctcgg
    45721 ctggcgcgtg aatggtaatg ccaccatgac gccgacgttt ggcacgctgg caagcccgca
    45781 aacctacggt cacactggct ggaccggaac ggtgaccgtt atcgatccgg tgaatcatat
    45841 ggcgattgtg atgttaagca acaagccaca ttcgccggtt gccgatccgc aaaagaatcc
    45901 caatatgttc gaaagcggtc agctgccgat tgcaacgtat ggttgggtag ttgatcaggt
    45961 gtatgcggcg ttaaagcaaa agtaataaaa aggccagtca gtaatgttac tgcctggcct
    46021 ttaatcaatg aatcagaaac gcttacagcg ccatcaactt gtccagcgac ggtgcgaaat
    46081 aatagccgcc ggttaccggt ttggtgaaac gcaacatcgc atcacgctta ccatcggtat
    46141 cgccaaacat gctcagcagt tgctgctcaa tgttatgcag acgcgcgcag taggcgcaga
    46201 agtacagacc gtgagtgcca ctggcggtgc cgtacggcag gctctggcga acaatcttca
    46261 gccctttgcc atcttctttc agatcaacgc gggtcaggtg agaggtttcc ggacgttcgt
    46321 cgccgtcaat ctcttcgttg gcctctttgg tgcgcccgat catcatctcc tgatcgtgaa
    46381 cgctcatccg gttgagctgc ttcaggttgt gctcccaacg ctgcacaaac acatagctgc
    46441 cgccggcatc cacgccgtct ttgataaccg ccacttcgcg acgcgtctct tcacccgccg
    46501 ggttttctgt gccgtcaaca aagccgctca ggtcacgctc ttccacccaa cggaagccgt
    46561 ggatctcttc tttcacttca atgcagtcac caaaggcttc catcgccgcc tgggcgacag
    46621 agaagtttac gtcgtgacgc agagaaagaa tgtggatcaa cacatcgaac tgggtggtcg
    46681 gcgcaaggcc tttaccgtag cccggaaaat ctttcagctc ttctgcccca acgccgccgc
    46741 tcagagcgcg ccagatgttg ttaccaaagg caaccaccgc accaagatgc gcgtccggga
    46801 atttcgcttc aaaagttgcc agtttgtcgg caaatgtttt actggccgca cgcagggcgt
    46861 caacttcccc tttcacgttg gcttcgatcc aaatcgccgc gcggcaatgt tctggcaaaa
    46921 tgccactctg aacctgagac attgttcctc ctgaaaataa taatgccacg caatccgtgg
    46981 cattgttgag cgctatttta cgtgtttttt cgcggcggcg gtttgttcag acgcaaatta
    47041 acgccgccag ataattttac tgactttcca gtttttcagg gtatcgtccg aaggcattaa
    47101 cccttcagga ccactccatt ccccgctgaa ctggtagcta atatgttgac tgccttcggc
    47161 tttacattcg acggcacgat tatcatcacc atcagctttt tggcaattgc caaatgcttt
    47221 gctgtaaagg tcgctaaacg gtgtaccgat tttaacaccg gtgtcagcag gaatatcgct
    47281 atccagcacg tcaatgcgac tgatcgtgcc ttgatcgccg ttaatcacca tcgccacgtt
    47341 gtcgcccttc atcacttcaa aaaagcgcac cacgttgccg ttcgcggttt tcattccgct
    47401 gcgcaggcga taatcgccat caaccgcatc ggcaatggct tgttcttgca gtggtgtgga
    47461 cgccgttaat tcaccaacgc cctgctcgct cactttggtg gacgacccaa accagttcca
    47521 cgggttagcg gcagaccagt taactgacga catcgtggaa cagccggtca gcatcaatgg
    47581 catagcgcat aacattaaac gcagcgattt catgtcactt cctttggtta ttcaataacg
    47641 ttgcttggag tgcaaattca ccaaaaagtg ccgttattct tttacttcat taaaacaagc
    47701 gcgtaaacgt cgattggtca gcagccagat taacgccaca atatccgcca ctaccagcgc
    47761 cagaccgata ccgctaacgg attcaccgtt cagccacagc cacggttgcc agcaaagtaa
    47821 aaccacctgc gccaacagca acagaaaata gagtacacgc caggtgcgag gaaacgtagc
    47881 ccgccgaccg ctcaacaaaa acgccagcac cgccggaatg ccaggaatca gccccagcca
    47941 gaaattatcg tgatcgggat aaaacagatt tagcagcgca gtaccctgct cgcgcgacgc
    48001 accggcaatg acaaacagca cccaggttcg cgcctgaagc aatagcacaa gccagaagag
    48061 caagggtaaa cgcaggcgac cgtgcgcatc ataatggaca ggatgaaact cagtactctt
    48121 catcttcaat caaacgctta cccagactca gcacgtcggc gtgttcatat cccaggcgtt
    48181 catacattcc gagcaccatg tcgttatctt ccggcacgtt gatctgaatt ttcgggcagc
    48241 cgcgagcaat cagctttttc tccagccggt taagcaacgc attggcaatc ccacgcccac
    48301 gaaactctgg atgcacgcca agataataag cagacccgcg atgcccgtca taaccgccca
    48361 tcaccgttcc gaccacttca ccgttcacct cagcgaccag aaacaaactg acgtcatggt
    48421 tcatcttacg ctcgatgtcc atttccggat cgttccacgg acgcagcaag tcgcaacgct
    48481 cccaaagggt gatgacctct tcgaaatctt cctggcgaaa tacgcgtatc tccatggtat
    48541 tcgttacctt tttgcgggtt aaaaggctga ttatggcgtg aacggtcgaa ttagccaata
    48601 tctgacgaaa atcggttgaa aaagtggcat aatggggagt tgtcaactat tgaaatgaaa
    48661 agtaaaacaa ttctcaacag caaacggtcg taacggatta cgcgatacga tataacatct
    48721 ggaactttat tattacaact caggccgtat gagcactttt aaaccactaa aaacactcac
    48781 ttcgcgccgc caggtgctga aagccggttt ggctgccctg acgttgtcag gaatgtcgca
    48841 agccatcgcc aaagacgaac ctttaaaaac cagcaacgga cacagcaagc cgaaagctaa
    48901 aaaatctggc ggcaaacgtg tcgttgttct cgatccaggc cacggcggga ttgataccgg
    48961 agcgatcggg cgcaacggtt cgaaagaaaa acatgtggtg ctggcgattg ctaaaaacgt
    49021 ccgttccatt ttgcgtaatc atgggattga tgcgcgttta acgcgttctg gcgatacgtt
    49081 tatcccactt tacgatcgcg ttgaaatcgc ccataaacat ggcgcagatc tgtttatgtc
    49141 aattcatgcc gatggcttta ccaacccgaa agctgccggt gcttcggtat ttgccctctc
    49201 taaccgtggg gcaagtagcg caatggcgaa atacctgtct gaacgcgaaa accgcgccga
    49261 tgaagttgcc ggtaaaaagg cgactgacaa ggatcatcta ttgcagcaag tgctgtttga
    49321 tctggtgcaa accgatacta ttaaaaacag cctgacgctc ggctcgcata ttctgaaaaa
    49381 gattaagccg gtgcataaac tgcacagccg caacaccgaa caagccgcgt ttgtggtgtt
    49441 gaaatcgccg tcggttcctt cggtgctggt ggaaacctcg tttatcacca acccggaaga
    49501 agagcgactg ttaggcacgg cggcgtttcg tcagaaaatc gccacagcga ttgctgaagg
    49561 cgtgatcagt tatttccact ggttcgacaa ccagaaagca cattcgaaaa agcgataagt
    49621 tatgaaaccc gacgcacacc aggttaaaca gtttctgctc aaccttcagg atacgatttg
    49681 tcagcagctg accgccgtcg atggcgcaga atttgtcgaa gatagctggc agcgcgaagg
    49741 tggcggcggc gggcgtagtc gggtgttgcg taatggtggt gttttcgaac aggcaggcgt
    49801 caacttttct cacgtccacg gcgaggcgat gcctgcttcc gccaccgctc atcgcccgga
    49861 acttgccggg cgcagtttcg aggcgatggg cgtttctctg gtagtgcatc cgcataatcc
    49921 gtatgttccc accagccacg cgaatgtgcg cttttttatt gccgaaaaac cgggtgcaga
    49981 gccagtctgg tggtttggcg gcggcttcga tttaacccct ttctatggtt ttgaagaaga
    50041 cgccattcac tggcaccgca ccgcccgtga cctgtgcctg ccatttggta aagacgttta
    50101 tccccgttac aaaaagtggt gcgacgatta cttctacctc aaacatcgca acgaacagcg
    50161 cggtattggc gggctgttct ttgatgatct aaacacgcca gatttcgacc actgttttgc
    50221 ctttatgcag gcggtaggca aaggctacac cgacgcttat ttaccaattg tagagcgacg
    50281 taaagcgatg gcctacggcg agcgcgagcg caattttcag ctctaccgtc gcggtcgtta
    50341 tgtcgagttc aatctggtct gggatcgcgg cactctgttt ggcctgcaaa ctggcgggcg
    50401 caccgagtcg atcctgatgt caatgccgcc actggtacgc tgggagtatg attatcagcc
    50461 aaaagatggc agcccagaag cggcgttaag tgagtttatt aaggtcaggg attgggtgta
    50521 actccctcac ccccattccc gcatccgctg atgcaacgtc aacgacggct tctcggcaaa
    50581 cagctgctgg taatccgtgg caaattgccc cagatgccag aatccccact gcatggcggc
    50641 gtcttttacc gttgtgcttt gcgaccacgg gcttatcagt tcgcggcgta cggcgttcag
    50701 gcgaatgcgt ttcagccacg cgttcgggcc aattcctaaa atagcgtgaa acgcgttttg
    50761 tagcgtgcgg cggctgacat gcagttgatt acacaagtcc agcaccgtca ccggttcgga
    50821 catgttttcc agcacatatt cacgggcgcg ggaaagcaac cgacggtaac tctgatgact
    50881 gatgctttcc gccgtcacca ttggctgcgc ttcttccagc atcgccccca tcgccattag
    50941 caaattatcc cccagcactt ttcgcactgc aggctgatgg agattttccg ggttctcgca
    51001 aaacgtcgcc agcgcctgtt ggacaaagcc ccacagcgcg gctttatgct gctctttcac
    51061 ttccagcgcc gactggctac gcaacatatg taatacccga tccggattat gcaaaaagtt
    51121 agcctgacgg gtgatgacat cttcagaaag cactacgccc aggatcgtgt aatcgtccgg
    51181 cgtacttaat tcaaactcgg ttccaccagg gcgcgtcgct atttctgcgc ttcccagaca
    51241 ttgcgaaccg ataaatccct gctcaccgcg cgtcgccgga atgccaaacc agaacgagtt
    51301 cggccagacc agacacgact gacgcagcgc cagaccggtg tattcacgaa aaacctgaat
    51361 atcatcgagt agaatttccg taaattcacc atgaaacttg cccggatgca gctgatcgta
    51421 aatctgctgc caggcggtaa tcgttaaagc atgttcatag acatccgttg tctgtcgttg
    51481 atgaacatta tccacttcga ccttcggcgt gagcttcagg ttttcgggta agggttcatg
    51541 ataaagatgg tgcaaattgg ctgtacgggt ctttttcatg atgttaatgc cgggtgttgt
    51601 aggacacccg gcacctccga caggttaatg gggcttgaga cgataacgac tactgcgttt
    51661 acgtaacgtc ccagcagaaa agagctgttc gagcgcattt ctggcttttt ccagcggcca
    51721 gccaaagtgg gcagccactt ctcctgccgt cactccctga cgtactgatg ttaacagcgc
    51781 caacaactcg tcagcagatt ccgcgacgat aactggcgcg tcgggttcct ttacgggttg
    51841 cttcggctgg cggttaaagc cagtaaccag ccactgggtg tcatcgtctg gccgaccgat
    51901 ctccttacgg ctgatgacac gcccggtacg catcgcggca gcacaacctg cgtccagcgc
    51961 agcacgacac gccgccagat cgccttccac caccagcgtt aagcgacctg ggtcaagcac
    52021 ttcgtgactg agcagacgaa cgttagctgc tttgagcatg gcatccgctg catctatcgc
    52081 ggcgaccatg ccgtccactt ccagcaatcc cagtgcattg atcattggca acctccgtta
    52141 cgcacgctgg attggattac gcgcgatttc cagcactgca tcggtaaagg cgttacaggc
    52201 cgctttacac gctgcctggc taccggttaa aaatgctgcc gagtagttag tttcagacgg
    52261 tggcgggaca taggtcacca gctgtacgtc ggcagatttc aacgctgcat caatgccgta
    52321 ggtcgcttcc agcggcggtg ccaccagata cgccatcgga tcgccaagcg tgatcccggc
    52381 ggttgatgag agataagaac cggtacgcga aactacatgt gccaggaatg ccgtatcttg
    52441 cgcatcatta gcccactgga aagcagcgcc attttcaata tgcgcaacca tcgcatccag
    52501 accggcacgc acttccgccg ggtttggccc accaagcata atcagcacct caccggcagt
    52561 cggtgacggg ccgtgtgccg cgcccgcata cagcgagcga ccatatacca cttctaccat
    52621 cgcttgtttg gtcgcttcgt cagccgcaat gtaggttacg tcgtcagaat cggcagaaat
    52681 cagaccgagg ctacgaatat gtggcggcaa tttcagttca cgcgcaaaat cggcgttaac
    52741 ggaggcaatc acccgcatgg cggttaccga cggtcgaatc aaatctaaag ctggcatgat
    52801 gcctccttat cgggtcatgt tgatgccgga tgctttctgc tccagcatac gtttcgccaa
    52861 atccacaatg acggctgcgg cttcaaccgg cggcgtgccg ccctggtgaa tgttagagat
    52921 acaggtgcga tcggcctcaa cggtggtcgc catacgcggc gagtaaacgg cgtagcagga
    52981 gaggctttct gactgaccca accccggacg ttcgcccacc agcaggatca ctactttcgc
    53041 gccgaggatc tcaccgatct gatcttcaat cttcacgcga ccataacgaa caaagaatgg
    53101 cgtgccgact ttcagcccgg cctgtttcag gcccgccatc agcggcggca ggatctcttc
    53161 gtagttcaca gtgatggcat cagttgacag gccatcagaa ataacgacct gtacgtccgg
    53221 attagcaaca cactgtgctt tcagcgcttc aacagcttct gcacacaggc ggcggcccat
    53281 atccgggcga gtcaggtaga gatttttgtc gctaatttcg gagcgtactt ccagcaagcc
    53341 ctgcgctttc acccactctt ccggcacttc tttcagtacg gtatctttcg aacgagagtg
    53401 atcggccagg aaacgcagca gcgccagcgt acgcggacgc ggaccggcac gaccggtaca
    53461 aacgcgggcc acggtgctgc gacgcagttc tgttaacaca tccgcacgat gtgggttttc
    53521 aacaccaatc cacacttttg cttccgcaga acctaaatcc agcgcgcagc tttccgaggt
    53581 caccggtgcc gcacaggtgg tagtggcgca ctttgcttct gacggcgcgg gggccgtttg
    53641 tcccattgac gccatcacgc tgcgtacaat ttcttcaatc tgtttttgat ccatgatgtg
    53701 ttatctccgc gtcatcagaa gaacagtgac ggatcgcccg cccgtttggt caggcgaccg
    53761 tttgccataa tgcccatgct ttccagccag cgttcaaact ccggtgacgg gcgcaggttg
    53821 agtaactgac gcacagtggc ggtatcgtgg aatgcggtgg tctggtagtt gagcatgatg
    53881 tcatcaccca gcggcatccc catgatgtag ttgcagcctg cggtggcgag caggatcatc
    53941 aggttttcgt tgaggttctg gtcagcgtca gcgtggttgg tgtaacagca gtcacagccc
    54001 atagagatgc cgctcagctt gcccataaag tgatcttcta agcccgcacg gataatctgg
    54061 cggtcgttgt agagatactc cggcccaata aagccgacca cggtgttgac gataaacgga
    54121 tcgtaatgac gcgccagccc atagttacgt gcttccatcg tcacctggtc tgcgccgaag
    54181 ttagcgccag cggatagcgc tgaaccttgt ccggtttcga agtagaggca gttttccccg
    54241 gcgatacagt tgaactctgc acccactgcg cgcgcttcgt cgagcatcgc cagttccacg
    54301 ccaaactctt tcagcccttt ttcgctgcca cagatactct ggaaaatcag tccgccaggc
    54361 gcgccgcgac gaatcgcttc gatctgggtg gtgacgtgcg ccagtacgca gccctgagtt
    54421 gggatgttga atttgtcgat cacgccatag atggtgtcca gcacgcggct taagttttcc
    54481 acgtcgtcag tcaccgggtt aacgccgatc accgcatcgc ccaccccgaa ggaaagccct
    54541 tcgtagattt gcgcggcgat actttgcacg tcgtcacggg tgtcatttgg ctgcaaacgg
    54601 gcgctgaagg tgcccggaat accgatggtg gtattggcct ttttgattac cggcattttc
    54661 ttcgcgccgt agatcaggtc cgcgttggag caaatcttcg ctaccgccgc gaccacttcc
    54721 gaggtcagcc ctttgcgggt aaaggcaatg tcgtccacgc tggtttcatc gctcagcaca
    54781 tactcacgca gttcgctgat gctccagttt ttaatctggt tgtaggccgt ttcgttaaca
    54841 tcgtcctgaa tcagccgcgt cacgcagtca tcttcatagg caatcaccgg attattgcgg
    54901 atgtccgcta cggtcatttc cgacaacacc tgctttgccg ccacgcgctc ctgtgagctt
    54961 gccgctgcga cgcccgccag cacatccccc gaacacagtt cgttggcttt agccagcacc
    55021 tcttttacat ccttaaactg atatacattg ccgaacaatg tggtctttag tttcataagt
    55081 cgttccctca ggaaggaaat gcgagtgatt tcaccgtcac cggcacaacc gatccgccaa
    55141 aaagaggcgt accaatgtcg atatagtccc ccgcgcggac aatcacttcg tcaatgactg
    55201 ccaacgggag ttgttgtagc tgtgggcgca acagcatgcc cagagcttta ccaaagtcct
    55261 gcccggccac caccagcagg ggatgcggat tcggaaaacg cgcgacgaaa tcgaccaacg
    55321 cgttgatgac cgtcagtacc gcggcgtaac gcacaggcag cgaggcggga agcgccagca
    55381 cgtacgcgtc agttttggga tcaagatcca gctgaatcag cgcctgttgc caggcactca
    55441 ccagatccgt ttcatcaatc gggatcgcca ccggcaaatt gcgcagcggc agttgtacgc
    55501 cctccagcca gattgtgctg ccagagagcg aaagggtatg tgcgcccgcg ccaatcaccg
    55561 tggcgcgcac ggtttgcgcc ggaaactgca cgttcatctc acgcaggcgc ggatggtcat
    55621 gtagcgccgt cgccagtagc gggccaatat cggcaaaaca gaacgggtca gcgggctggt
    55681 ggcgataaca ttcgcccacg ccgccagaaa gcgtaatgat ttcgggcgta acacctgcgg
    55741 gcagcaaacc ggtttgcatt aatgcctgcg cgagcggcga aagcgttccg tcaatcactt
    55801 cgacaatcag ctctgccatc cgtcgggtaa cctgcaccag ctgcgcgccg gtcagcgaac
    55861 ggacgtcagt gcctgcaccg aaacactcat ccacaatcat ctgccccggt ttatgagcgt
    55921 aaaccacgcg cccctggctg tcggtttcca gcaggcgacc accgacgttg aggcaggcag
    55981 tgccgctgat ttttccggca tcgaacaggg cgtagttcgc ggtgccaccg ccgatgtcga
    56041 tattcagtac ccgacacagc cgttgttcag aaagggtttg cgccccggca ccgtgaccgg
    56101 cgatcacgga ttcgaggtgc ggcccggcgc tggcgacgac aaaatcgccc agcgactgag
    56161 agagcgccat caccgccggg cgagcattgc gggttttcgc gctttcaccg gtgatgatga
    56221 tggcaccaga atcaacgctt tccggcgcaa tgcccgcagc ctgatattgc tctaaaatca
    56281 gcgatttcag ttccgcttct tttaaaccac cctgtttatc gacaggggta aagaacaccg
    56341 ggctttgcca gctaatttcg cgtttgatga attcgtagcg cggcacctgc gacaccgccg
    56401 cacggttaac cagttccagg tgggagaaaa tcacctgggt ggtggtggtg ccgatatcga
    56461 taccgacgct cagtagctgg cgagtgttca cgattgtgcc tccgcttcgg ttttagccgc
    56521 ggtcgcgtcg tcttttggca ccagcatcat cgccacgcca atcgccgtta cgccgccgat
    56581 caacttgccg acaatcatcg ggaagatcat ggcattcatg ttggcagcgg cgaagcctaa
    56641 gtggtcgccc agggcgaaag cagcggaaac ggcgaaggcg cagttgatga ctttgccgcg
    56701 ggtatccatc tgcttcatca tgccgaacat cgggatgttg ttggcaagcg ttgccaccat
    56761 gccggctgcc gcgatgttgt tcatattcag cactttaccg acgctcatca gcggtttttc
    56821 aaaccagcgg gtcagcagca gcaccatcgg atacgcccct aacagaacgc aggagataga
    56881 accgataact tcaatggcgc gcatcacctc accgggttta tcgccagggg ccataaagat
    56941 aggatccaga ccggggatca gttcccagcc aagcaggaat ttcactaccg cagcggcaag
    57001 accgagggtg atcaatgcaa cgaggaattt ggcgaagatc tggaagccgt tgatcatttt
    57061 ttctgggatg aatttcagcc ccagcgccac cagaatcgca acaatgatca ccgggatcat
    57121 gttcatcagg atcagggcga aagtgaattc caccggctgg ccgttgatct gaacaccgga
    57181 gtacatagca accagaccac cagcgataca accaatcgga atggtcacaa tgcccgccag
    57241 cacgccgagc gccagataac gacggtcaga aggttcgata atgccgagcg ccaccggaat
    57301 ggaaaacaca atcgttggcc ccatcatcga cccgagaatt aacccagagt atagccacgc
    57361 ggctacgtcg ccgcccgcca gctctttggc gaggaagaag ccgcccatat cgcacgccag
    57421 cagtgttccg gcgaacatcg atgggttagc gccgagcatt tcgtaaaccg gaataattac
    57481 cggcccgagt acgtgagcca gtaccggtgc cagcgcggtc ataccgacca tcgccaggcc
    57541 cagtgcgccc attgccataa agccttcttc gaactgaccg ccggatcctt cgatactttt
    57601 accgaactta ccgaggaaac gagcagaacc gccgaactgt gacaggatcc tgtcaacggc
    57661 agctatcagc ataaagaaca tcatgatgta catgatgatt tcgttaattc ccatcgcctt
    57721 tactccctgt tagttgttat ttattagcgg atgctgcgtg aagccttatc cgcactacat
    57781 gtgcaatccc acagaccgga taagacgcgg catttattgc gccgctgcgt acaggccgac
    57841 aagctgttcc aggctggcgg tacgcgggtt gctgcgcaga caaatatctt ccagcgcggc
    57901 ctgcgcccat gcaccgtaat gcgcagatgt cgcgccaaca tcgcccagtc gtttaccaat
    57961 cccaacttcc gcaatcagct cacttaccgc gttaatagcg tcacgatcgt cggatttttt
    58021 agttcgcagt gcccgaccaa tctgactaaa gcgttcgcga caaaccatcc ggttaaactc
    58081 catcaccgtt ggcagcaaca tagcgttcgc gagaccgtgc ggaatatgca gcgccgcgcc
    58141 cggctgatgc gccatcgcgt ggcacaaccc aagacccgca ctggaaaacg ccattcccgc
    58201 catacatgaa gccagcaaca tgctctcgcg cgcggcaagg tcgtgaccgt agcccaccgc
    58261 tttcggcagc gatttgccaa tcatcgcaat ggcaccaatc gccagactgt cggtaaacgg
    58321 tgtagcgttc agggcgctgt atgcttcaat ggcatgggtt aacgcatcaa tgccggtcat
    58381 cgccgtgaca tgcgacggca caccttcggt caatgcggcg tcgaggatcg ccacatccgg
    58441 catcagcgag gcatgggcta acacctgctt gcgcccgctc accgcgtcga taatcaccgt
    58501 tacattggtg gtttcagagc cggttccggc ggtagttgga atggcaatca gcggcaagcg
    58561 cggttgcaga acgctggttt ctgacatctc tgccagcgtg ctatccgggt tcgtcaccag
    58621 caacgccacg gctttcgccg catccagcac cgagccgccg ccaaatgcga tcaccccgtc
    58681 acaacctgac tcacgcaact gcgccacggc tgcacacacg tcggtaatgc acggttcgcc
    58741 caccggacat ggccagagcg tcatggcgat acctttaacg gccaggctac gcgtcagccc
    58801 ggcggtcatc cctgcctgat gcaaaaagct gtctgccatc acgaacagat gtttcagccc
    58861 acgcgtttgc gcttgctgtc cgcaactgct caccgagccc ggaccgcaaa gcgtcaccgg
    58921 tggaacgcta aatgttttta cccgttgcag attcagggta tcgaacgcct gaaagagcgc
    58981 ggtctgcaat tcattttgca tatagcccct ccgctttctc tcgcccacta ctggcgatcg
    59041 ccagcggggt cataaacaaa ctgtgctgcg gtaaatgcac ctgtaacgcc gggaattgtt
    59101 tgcgaaacag ttctgccacg cccggttgca tacaggagcc gcccgccagc cataaatcag
    59161 taatgccctg cccttcaata tgccgggcga caatgtccgc cattttttcg tacaccggtt
    59221 tcaccgtagg ccaaatctct tcaccgtgac cgcgcttgta ctgctccgcc tcttccagcg
    59281 aaatacggcg atttccggcg aaagtcagag agatgtgatg tccgccggtg gcttcatccg
    59341 ccgagtacgt cactttgccc tttttcacga tggcaatgcc ggtagtgcca ccgccgatat
    59401 ccactacacc ggcgttgtcc agttgcagca gatccgcgac tgcggttggc tcatccagca
    59461 catggctcac ttccagcccg gcagactcca gcacgttgat ggaaatacgc gggtccgtac
    59521 ctggaggaaa tgaagtcgcc gcatggctaa aacgacgacc gaattgctgc tcgagcgtgt
    59581 cgagatggcg acgaacaatg gtgaccgcgc cgaagaaatc ccagacgatg ccgtcgcgaa
    59641 ccacatcggc ccagtcgagg cataccgcca ctggctgacc gtcgcggtcg acaaccatcg
    59701 acaccacatc gcaggtgccc agatccactc ccagccacag cggagattcc gtcgcggcgg
    59761 gcgtctggtt acacagcgtt gccgccgttt gcagacgtgg ggtgagccat tgttcgtcgt
    59821 gcgccatctg ttactcctta aacaatgcga aacgcatcga ctaatacaca gcgacgcaga
    59881 cggacaaacg tacgcgcgct ggtcacccct tcaccggttg gcgtggtgat ggtcatggtg
    59941 gtccagcctt ccccgcccag ccccagcccg gcaatgcacg gtccgttctt aacgaaaatg
    60001 ctggtatcaa cagcattcgc catctggttc atgttttcga tgttgcgcga gtgcattgcc
    60061 gccgtgtggt ggcaaccgcc ttccagtttc accgctagcg caatggcatc cgccacgttg
    60121 gcgacgcgca cgacgggcaa caccggcatc atcagttcag tcacggcaaa cggatgttct
    60181 gcggtggttt ccacaaacag caggcgcgtt tcttgcggaa ctttaaggcc gattgccgcc
    60241 gcgattttgc ctgcgtcgcg accaacccag tcacggctga cggtgccttt tccgcgctcg
    60301 tcgatatttt tcagcaacac cggttgcagc tgctgcgcct gttctgcggt cagtttcacc
    60361 gcgtgctggc cttccatcag acgcatcagt tcatcggcta cgctatcaac aacaatcagt
    60421 accttttcgt cggcacaaat gatgttgtta tcgaaagacg cgcctttgac gatggactga
    60481 gcagcacggg cgaggtcagc ggtttcatcc accactaccg gcgggttgcc agcgcctgcg
    60541 gcaatcagac gtttattggt gtgtttacgc gccgcttcta ctaccgcttc gccgccggtc
    60601 accaccagca ggccgatacc cggaaacttg aacaagcgtt gcgcggtttc gatatccgga
    60661 tttgccacag taaccagtaa gttttccggc ccgcctgcgg caacaatcgc ctggttgagc
    60721 agcgtgatcg cccgctggga gacttttttc gccgccggat gcggggcaaa aatgacgctg
    60781 ttgcccgcgg caatcaggct gatggcgtta ttaattacgg ttgccgccgg gttagtggac
    60841 ggcgtcaccg aagccaccac gccccagggc gcgttttcaa ttaaagtcag gccgttgtcg
    60901 cccgtcagca cttgcggaga gagacactca acgcctggcg tgccgcgtgc ctgagcgacg
    60961 tttttggcaa atttatcttc aacgcgaccc atgccggttt cactgacggc aagttccgct
    61021 aaatctctgg cgtgtttttc gcctgcttca cgaatggcag caatggctaa ctggcgcatt
    61081 gccacgctct ttaacccttg ctgggcaatt ttggctgccg caacagcgtc atccagggac
    61141 gcgaaaacgc ccatctcatg aacggcggcg ggcggcgtgt cactgctttg cattttcagc
    61201 agtaccgctt tcaccacctg ttcaatatcc tgttgattca tgatgttctg ccttatttgt
    61261 ggaaaattac ctgaccgcca gacaccacct catcgacgat gccaatcacg cacagatcga
    61321 ccggtgacgt ttcgctttta tgcgcctggc gggcggaact gccactcacc agcaacaccc
    61381 actccccggt tcccgcgcca atattgtcga tggcgacggc acattgcccg tcaggattac
    61441 catgtggatc aatcatttcc accatcagca atttgtcatg cgccagtccg tgatggcgta
    61501 cggtacaaac aatttgtcca gtgacgactg ccagtttcat acccgcctcc gtggcgtatt
    61561 tcaggtaaaa gctcccccta ccctccgcgg aaggtaaaat gaaaaatgag agagcgtgac
    61621 gcccgaatcg acgtcacaca gggtgattac aggttgctgc tatcgccttt caggccgatc
    61681 gggaacactt cttccaggtc gccgtgcgga cgcgggataa cgtgtacaga taccagctcg
    61741 ccgatacgct gtgccgctgc ggcaccggca tcggtcgccg ctttacaggc tgcaacatca
    61801 ccacgaacca tggcagtaca caggccgccg ccaatctgct taacaccaac cagcttgacg
    61861 cgcgcagctt tcaccatcgc gtcagaagcc tcaatcagtg caaccaggcc ccgggtttcg
    61921 atcattccta atgcttccat tgtgctttcc tctttatcag ggtccagaac gggaccgttc
    61981 attcaaccag tgtttgtaaa ctgctttcgc ggttcacttc tgtctgacgc ggcacagctg
    62041 ccaccagcgc cagctcgata atttcctgca cactacaacc acgagagaga tcgtgcatcg
    62101 gcgcggcaag tccttgtatc aatggcccga cggcacgata tccgccgagt cgttgtgcga
    62161 ttttgtagcc aatatttccg gcttccagcg acggaaaaac catcacattg gccttgccct
    62221 gtagcgggct ggcaggcgct ttttgcgccg ccacttccgg cacgaaggcg gcgtcaaact
    62281 gcaactcgcc atcgactacc agctttggtg cgcgctcacg gacgatttct gtcgcctgct
    62341 ggacattggc aacgcagggg tgacgggcgc taccgttact ggaaaacgac agcatcgcca
    62401 cgcgcggctc ttctccggtg atggcgcgcc aggtttcggc gctggcaagc gcgatatccg
    62461 ccagctgcgc cgccgtcggc tgtggtacca cgctgcaatc ggcaaagccc aacgccggac
    62521 cgctgtactg tggcagcatc aggaaaatgg atgagagcgt tttacagcct ggctgcaagc
    62581 cgataatgcg taatccggca cgcagcacat tcgccgtgga agagaggttg cccgcgatac
    62641 agacatccgc tttaccggcg ctgaccattg cggcggcgaa catcagcgga tcggtaagtt
    62701 tttccagcgc atccggcggc gttttttcgc ccgcgcgggc cagccagcga tgagcaaatt
    62761 cttcccgcat tgcgaggttg ccatgcggat ctatcacctg tagcccgtcc atcgccacgc
    62821 cgtgactgag cgcaaactga cgaagttcaa acggattggc gaccagaatg ggcgttgcca
    62881 gaccttgttg atgtaaatat tgcgcagctt tcagcacacg ttgatctaac gcatccggaa
    62941 aaaccactct ggcgggcgct cgcaacgcca gttcacaaca acgttcaata atcattgcgt
    63001 ttcccccagt cgttgttgga tctgcttcac cgttagcggc tgcttcgtta cgctcaggat
    63061 catcatcacg tagaccgtgc tggaaaggcg gttcagcgcc tgcaagatgt ccgggcgtaa
    63121 tacttcaaaa ctgcgggtga taaacacctg cgccgctacg gtttcggctt cccgcacttt
    63181 ggtgcgtagc agattcagca acgccgcatc gcgcccgtgg cttgcttccg gcaccagatg
    63241 atcgtgacca agataacgca acggctgatg agaaagccgg tgcagatctt cgtcagaaag
    63301 cccgacaatc gcctggcagc ccagcggttc cgccagttca atttgcagcc acactgccag
    63361 cgcaatggtg ctgtcgagaa ctgcgcgaaa acccagacgc ggatcgcttt tggcgaccat
    63421 tttttccgcc gacaggtgag tcagcgtatc cggctttttt gccaccggct ggcggcacag
    63481 ttcgcagcac gcctgcggat gttcatcgct actggttagc ccatgaacgg gctgcggctg
    63541 ctgctgttca tcgtcaacaa acaggcggcc ctgctcgtca ataaacttga tgcgcagatg
    63601 gcggctttcc agcaactccc gggcagaggg cgtcaggcga ctgtccgcag ggagatggat
    63661 ctctgcgcct tcgctgagcg tatggttcgc tcttagccat gcttcggtga tgaaatcttt
    63721 catagggatt gccagttagc cggccaggcg acatacagga atttcacgct ggatgtcgta
    63781 ccaaattcga tgctggagcc tttcgggata aacatcacgt cgcctgcttt agcgatcatg
    63841 gtttcgcctt cgtggcgaac atggagctcc ccttccagca ccatgtcgat ttcgtcgtag
    63901 ttcagggtcc acgggaagaa tgcgttctcc cactgcataa aaccggcggc catgctgctg
    63961 ccgtcgtctc cggtcacaag atcagtcaag cccacgcaat gcggttgtgc gccatcaaag
    64021 cgaccaaact tgacgctgct accatcaatc actttgatgc cgcctttgcc ggtcaccgat
    64081 ttgaagctcg gctgcattgc accctgctcc agcgactgtt tttccttcat cactttttcc
    64141 atcagctgcg cgaccaggct ttcggtaaac tggccttccg gtagctgggc gataatggtt
    64201 tcgcggatgc gctggctttc ggttttatcg gctggcacgc tggcgggcac ggacgccgtt
    64261 accgggatca attcatcaca ttcggtgatg gtaaaaccca gtagatccgc cacttcacgg
    64321 gcttccgggg taataatgct ggcgcgcaga accacggaca ttgcctgttc gccgcgcgcg
    64381 tgtgcttcac gaatatcatt cgctgtgatg agttttttca cctgcttcct cctgtttggt
    64441 gagagatgcc agataatcca ccagttgctg cacgctttgc gggtcgtggc tgtttagctc
    64501 gaaaatcggc tcctcgaagc ccgtctcaag caataacttt cgcgttgcgg cgacgtcagc
    64561 atccggcatg tccgttttac tgatgacggc gatttgccgt ttgctgacgc caatatccag
    64621 caacccggca ggcaaccgac tttccgggtc attcgcgccg tggacataga tcagcatgtc
    64681 cacatcctgt agcgtggtaa ttaaggcgtg ataccagcgg ggatggctaa aatattcacc
    64741 cggcgtatca atatcgccct tatcattaaa ttccacggcc tgtgtttttc tggcgagggt
    64801 ataattcccc tgcaacgcat taaataacgt tgttttccct gccccgacgg tacccacaaa
    64861 cgcaattcgt ttcatgaatg cctcgtatta acttttggtc atttcacaaa gcgtataatt
    64921 taataagcga cctaaaccac tgacggtttg tgataacgct tcttctactg cgcccaccga
    64981 gccataaatc accaatgcac cactgaagcg gtcgagaaat ccgatatgca catcggcggc
    65041 tttcagcgct aaatcaccgg cgatcatcgc cgtttcgccg ggagttagcg tcataatgcc
    65101 aattgcgccc gcatccggaa cgccgatttt cttcgccagt tcctcgccgg gatgcgcgat
    65161 gagatgcgcc agtgtgacct gtttgccggg cacaaattcc tgaatgatgc gttctttatc
    65221 catgagtcgc cgcctgtgaa aaactttcgt gtatcgtgac aatttttc
//
LOCUS       DBFIFM010000005        64140 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.005, whole genome shotgun sequence.
ACCESSION   DBFIFM010000005 DBFIFM010000000
VERSION     DBFIFM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.005"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..1310
                     /locus_tag="N7C74_000267"
     CDS             <1..1310
                     /locus_tag="N7C74_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HGU2430214.1"
                     /translation="YQFIVTINSKNYGCPLDNTDINWCGIVYLLNNMTVNDNANDVAV
                     TESYKPIWNWKISQYNVSDIKFETIIKPQFADRIYFSNCSPVDPTSTRPTYFGDTDGS
                     VGAVLYALFATGHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQVDTNVNTIISIL
                     NRRDIILESLQPYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINDNV
                     HVLAGNHETNCNGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDSKYKIMANHHGITFDD
                     QRKRYIIGPITVSIDEMTNALDPVELAAIINKKHHTIINSKKFKTSRAISCRSFNRYF
                     SVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHTGRMF
                     SMHNCQINPSAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            1500..1613
                     /gene="yhiY"
                     /locus_tag="N7C74_000268"
     CDS             1500..1613
                     /gene="yhiY"
                     /locus_tag="N7C74_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HGU2430215.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1676..2743
                     /gene="yhiI"
                     /locus_tag="N7C74_000269"
     CDS             1676..2743
                     /gene="yhiI"
                     /locus_tag="N7C74_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU2430216.1"
                     /translation="MDKSKRHLAWWGVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2740..5475
                     /gene="rbbA"
                     /locus_tag="N7C74_000270"
     CDS             2740..5475
                     /gene="rbbA"
                     /locus_tag="N7C74_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HGU2430217.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVIVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEASPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPLLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPSSQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5475..6599
                     /gene="yhhJ"
                     /locus_tag="N7C74_000271"
     CDS             5475..6599
                     /gene="yhhJ"
                     /locus_tag="N7C74_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2430218.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLIMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6672..6947
                     /locus_tag="N7C74_000272"
     CDS             6672..6947
                     /locus_tag="N7C74_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HGU2430219.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            6944..7303
                     /locus_tag="N7C74_000273"
     CDS             6944..7303
                     /locus_tag="N7C74_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HGU2430220.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(7353..8213)
                     /locus_tag="N7C74_000274"
     CDS             complement(7353..8213)
                     /locus_tag="N7C74_000274"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HGU2430221.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTQEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVCPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRLVRKAS"
     gene            complement(8245..8514)
                     /locus_tag="N7C74_000275"
     CDS             complement(8245..8514)
                     /locus_tag="N7C74_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HGU2430222.1"
                     /translation="MLNKTVEVRNSTGLHARPAACLAKAAKKYSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTDLFRDRFGEAE"
     gene            complement(8504..10012)
                     /locus_tag="N7C74_000276"
     CDS             complement(8504..10012)
                     /locus_tag="N7C74_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HGU2430223.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQHTDITLASRTLCLDVRKGEWSAKAAALLHVPCTAFAPLVQPGEHAGWV
                     SESLCKTLGFSQPVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLTRGHADYLA
                     GNWLLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLSIDMLIASVSLGLTMEFANC
                     FACFNVPGTSALKVIGPATHNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIP
                     PPPVAIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVE"
     gene            complement(10005..11363)
                     /locus_tag="N7C74_000277"
     CDS             complement(10005..11363)
                     /locus_tag="N7C74_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HGU2430224.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALCFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGILLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNLIAIGLVPVIALVLYGIFRMTRSTV
                     YA"
     gene            complement(11440..11721)
                     /locus_tag="N7C74_000278"
     CDS             complement(11440..11721)
                     /locus_tag="N7C74_000278"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGU2430225.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEQGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKK"
     gene            complement(11718..12191)
                     /locus_tag="N7C74_000279"
     CDS             complement(11718..12191)
                     /locus_tag="N7C74_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGU2430226.1"
                     /translation="MQGIHFQENYIQRLPAGLSVEQIIRQLAQPLVAAELVVPDFADH
                     VLKREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVEFADMGGDPDPVPVR
                     VIFLLALSESNKQLNALGWIMEMIQDTPFMQALLAMETTEIHTVILNKMKERGEI"
     gene            complement(12216..12962)
                     /locus_tag="N7C74_000280"
     CDS             complement(12216..12962)
                     /locus_tag="N7C74_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU2430227.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LDVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIESANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIA
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPIEFGNVWLKGNKYYLGTVLQRREVS"
     gene            complement(13161..13562)
                     /gene="nikR"
                     /locus_tag="N7C74_000281"
     CDS             complement(13161..13562)
                     /gene="nikR"
                     /locus_tag="N7C74_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HGU2430228.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(13568..14374)
                     /gene="nikE"
                     /locus_tag="N7C74_000282"
     CDS             complement(13568..14374)
                     /gene="nikE"
                     /locus_tag="N7C74_000282"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HGU2430229.1"
                     /translation="MTLLNVSDLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLSRLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLHAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGEKLTFSSDAGRVLQNAILPAFPVRRRATEKV"
     gene            complement(14371..15135)
                     /gene="nikD"
                     /locus_tag="N7C74_000283"
     CDS             complement(14371..15135)
                     /gene="nikD"
                     /locus_tag="N7C74_000283"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HGU2430230.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(15135..15968)
                     /gene="nikC"
                     /locus_tag="N7C74_000284"
     CDS             complement(15135..15968)
                     /gene="nikC"
                     /locus_tag="N7C74_000284"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HGU2430231.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(15965..16909)
                     /gene="nikB"
                     /locus_tag="N7C74_000285"
     CDS             complement(15965..16909)
                     /gene="nikB"
                     /locus_tag="N7C74_000285"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HGU2430232.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFATQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFTWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(16909..18483)
                     /gene="nikA"
                     /locus_tag="N7C74_000286"
     CDS             complement(16909..18483)
                     /gene="nikA"
                     /locus_tag="N7C74_000286"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU2430233.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLHIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDEAQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(18594..19181)
                     /gene="acpT"
                     /locus_tag="N7C74_000287"
     CDS             complement(18594..19181)
                     /gene="acpT"
                     /locus_tag="N7C74_000287"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HGU2430234.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(19183..20412)
                     /locus_tag="N7C74_000288"
     CDS             complement(19183..20412)
                     /locus_tag="N7C74_000288"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HGU2430235.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(20409..21140)
                     /locus_tag="N7C74_000289"
     CDS             complement(20409..21140)
                     /locus_tag="N7C74_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HGU2430236.1"
                     /translation="MSRSVLVTGASKGIGRAIACQLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            complement(21140..21604)
                     /locus_tag="N7C74_000290"
     CDS             complement(21140..21604)
                     /locus_tag="N7C74_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HGU2430237.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFTLELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCTTGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            complement(21601..22770)
                     /locus_tag="N7C74_000291"
     CDS             complement(21601..22770)
                     /locus_tag="N7C74_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HGU2430238.1"
                     /translation="MIYISAVGMINALGNNLDEIATNLTRGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQIQPQVDDAIAKYGRERIAIVLGTS
                     TSGLHEGDTHVNLRTHGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAIISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGTAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            complement(22772..23356)
                     /locus_tag="N7C74_000292"
     CDS             complement(22772..23356)
                     /locus_tag="N7C74_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HGU2430239.1"
                     /translation="MIKSTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLTGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPAGWTLRDNGDKRELRNASGKL
                     VTEITYLNRKGKRVPISIEQHVFKYHITIQYLGD"
     gene            complement(23353..25671)
                     /locus_tag="N7C74_000293"
     CDS             complement(23353..25671)
                     /locus_tag="N7C74_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HGU2430240.1"
                     /translation="MTNANVLPPSKRPALLWGLVCLVMAVALLILLPQSRLNSSVLAM
                     LPKQTMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPLVAQEWLTLLQKSAALGDVKG
                     PMDAASQQAWGAFFWQHRNGLIDPNTRARLQNGGEAQAQWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKDLKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILLIVAV
                     FRSLRPLLLCVISIGIGALAGTVATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSLGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLRRLEKYTASLEYA
                     KKEGLISNYRTIPLNSLARQEEDLQLLKTAAPTVTKALQNAGLTAVNPDLNAMPVNVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALMQEIATYYPCGIAWVDRKSTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAIALAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            complement(25640..26245)
                     /locus_tag="N7C74_000294"
     CDS             complement(25640..26245)
                     /locus_tag="N7C74_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HGU2430241.1"
                     /translation="MKFLPLLALLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGK
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLTDDERQRFAAQ"
     gene            complement(26242..26664)
                     /locus_tag="N7C74_000295"
     CDS             complement(26242..26664)
                     /locus_tag="N7C74_000295"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HGU2430242.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVDEQSRELCFVSPDILFERMGVKP"
     gene            complement(26668..28344)
                     /locus_tag="N7C74_000296"
     CDS             complement(26668..28344)
                     /locus_tag="N7C74_000296"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGU2430243.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAT
                     TQQQLDSLLAEQPGVTLIRLAENAGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQQPAALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKHATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPLDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSCHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTASRARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQPGRDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVN
                     PQRGGDWRVCWSSFMGQPAPFPQGPFILASILRCPVNLIFALRQHGKLHIHCESFADP
                     LQLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            complement(28335..28688)
                     /locus_tag="N7C74_000297"
     CDS             complement(28335..28688)
                     /locus_tag="N7C74_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HGU2430244.1"
                     /translation="MKPHEIERHQAQTNHLEIVLHLRADLFWFRGHFAVQPLLPGVAQ
                     IDWAMSYALNLLAPGWRFHSIQNIKFQSPLLPENRVTLALNWQEERQILSFSYQRHDG
                     DARHTASSGKIRLCR"
     gene            complement(28675..30036)
                     /locus_tag="N7C74_000298"
                     /pseudo
     CDS             complement(28675..30036)
                     /locus_tag="N7C74_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase"
     gene            complement(30033..30614)
                     /locus_tag="N7C74_000299"
     CDS             complement(30033..30614)
                     /locus_tag="N7C74_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2430245.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFVIWFGLAHNGLHWLLPLMALL
                     LLLRLRQTRRQAGPLQAVTQLVAVVGIALCVASFMLKTHQLLLFYPVVVNAVMLAVFG
                     GSLWSAMPIVERLARLQEPDLPEKGVRYTRHVTQIWCGFFIINGGIALFTALYADMSL
                     WTAWNGMIAYLLMGTLMAGEWLLRRQVMKRDRA"
     gene            complement(30619..30870)
                     /locus_tag="N7C74_000300"
     CDS             complement(30619..30870)
                     /locus_tag="N7C74_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGU2430246.1"
                     /translation="MTEQQTVYQEVSALLVKLFEIDPQDIKPETRLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEA"
     gene            complement(30882..31139)
                     /locus_tag="N7C74_000301"
     CDS             complement(30882..31139)
                     /locus_tag="N7C74_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HGU2430247.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIDTDAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            complement(31114..31935)
                     /locus_tag="N7C74_000302"
                     /pseudo
     CDS             complement(31114..31935)
                     /locus_tag="N7C74_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
     gene            complement(31932..32654)
                     /locus_tag="N7C74_000303"
     CDS             complement(31932..32654)
                     /locus_tag="N7C74_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HGU2430248.1"
                     /translation="MKFALNILDWQARAPGLSETLQWQEWSRQPQAIDPTAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPSVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPSEMPTWPYAVALVIEAGDDWQCETQSAIAGNETTLPQSMLFLQH
                     YLQNVDTFSLPGERVQWRWSHR"
     gene            complement(32695..33753)
                     /locus_tag="N7C74_000304"
     CDS             complement(32695..33753)
                     /locus_tag="N7C74_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HGU2430249.1"
                     /translation="MYEQDSLSALDAITEAQRIAFAPMLFQTALCLRNAGVLSYLDRQ
                     GKHGATLADITEHSHINEYATSVLLDMGLSGRIITCKEGIYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWSTIYPALSQLPDAARESWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKYNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKVCIMELFWDAQRFEAASFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            complement(33822..34199)
                     /locus_tag="N7C74_000305"
     CDS             complement(33822..34199)
                     /locus_tag="N7C74_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU2430250.1"
                     /translation="MKWFAAIAVVGALAGCARTAPIDQVHSTVSAGHTQEQVKKAILK
                     AGVERKWIMSEAGQGIIKARQQSRDHSAEIRINYTASSYDINYENSQNLQASGGQIHK
                     NYNRWVRNLDKDIQLNLSAGADL"
     gene            complement(34591..35640)
                     /gene="yhhT"
                     /locus_tag="N7C74_000306"
     CDS             complement(34591..35640)
                     /gene="yhhT"
                     /locus_tag="N7C74_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGU2430251.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            35772..36989
                     /gene="yhhS"
                     /locus_tag="N7C74_000307"
     CDS             35772..36989
                     /gene="yhhS"
                     /locus_tag="N7C74_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2430252.1"
                     /translation="MPEPVAEPALNGLRLNLRILSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDVKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(36993..37550)
                     /gene="dcrB"
                     /locus_tag="N7C74_000308"
     CDS             complement(36993..37550)
                     /gene="dcrB"
                     /locus_tag="N7C74_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HGU2430253.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(37623..38288)
                     /gene="yhhQ"
                     /locus_tag="N7C74_000309"
     CDS             complement(37623..38288)
                     /gene="yhhQ"
                     /locus_tag="N7C74_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HGU2430254.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            38509..38754
                     /gene="tusA"
                     /locus_tag="N7C74_000310"
     CDS             38509..38754
                     /gene="tusA"
                     /locus_tag="N7C74_000310"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HGU2430255.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(38856..41054)
                     /gene="zntA"
                     /locus_tag="N7C74_000311"
     CDS             complement(38856..41054)
                     /gene="zntA"
                     /locus_tag="N7C74_000311"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HGU2430256.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFATSWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41128..41754)
                     /gene="yhhN"
                     /locus_tag="N7C74_000312"
     CDS             complement(41128..41754)
                     /gene="yhhN"
                     /locus_tag="N7C74_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HGU2430257.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            41895..42254
                     /gene="yhhM"
                     /locus_tag="N7C74_000313"
     CDS             41895..42254
                     /gene="yhhM"
                     /locus_tag="N7C74_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HGU2430258.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(42257..42526)
                     /gene="yhhL"
                     /locus_tag="N7C74_000314"
     CDS             complement(42257..42526)
                     /gene="yhhL"
                     /locus_tag="N7C74_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HGU2430259.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(42516..43112)
                     /gene="rsmD"
                     /locus_tag="N7C74_000315"
     CDS             complement(42516..43112)
                     /gene="rsmD"
                     /locus_tag="N7C74_000315"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HGU2430260.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADETLIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            43262..44758
                     /gene="ftsY"
                     /locus_tag="N7C74_000316"
     CDS             43262..44758
                     /gene="ftsY"
                     /locus_tag="N7C74_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HGU2430261.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQTVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAENEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            44761..45429
                     /gene="ftsE"
                     /locus_tag="N7C74_000317"
     CDS             44761..45429
                     /gene="ftsE"
                     /locus_tag="N7C74_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HGU2430262.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            45422..46480
                     /gene="ftsX"
                     /locus_tag="N7C74_000318"
     CDS             45422..46480
                     /gene="ftsX"
                     /locus_tag="N7C74_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HGU2430263.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            46725..47579
                     /gene="rpoH"
                     /locus_tag="N7C74_000319"
     CDS             46725..47579
                     /gene="rpoH"
                     /locus_tag="N7C74_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HGU2430264.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            47850..48953
                     /gene="livJ"
                     /locus_tag="N7C74_000320"
     CDS             47850..48953
                     /gene="livJ"
                     /locus_tag="N7C74_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HGU2430265.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            49103..49369
                     /gene="ataR"
                     /locus_tag="N7C74_000321"
     CDS             49103..49369
                     /gene="ataR"
                     /locus_tag="N7C74_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin AtaR"
                     /protein_id="HGU2430266.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            49376..49903
                     /gene="ataT"
                     /locus_tag="N7C74_000322"
     CDS             49376..49903
                     /gene="ataT"
                     /locus_tag="N7C74_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     acetyltransferase AtaT"
                     /protein_id="HGU2430267.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
                     FFPTKSIELLFTQSD"
     gene            complement(49900..50283)
                     /gene="panM"
                     /locus_tag="N7C74_000323"
     CDS             complement(49900..50283)
                     /gene="panM"
                     /locus_tag="N7C74_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HGU2430268.1"
                     /translation="MKLTIIRLEKFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            50707..51816
                     /gene="livK"
                     /locus_tag="N7C74_000324"
     CDS             50707..51816
                     /gene="livK"
                     /locus_tag="N7C74_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HGU2430269.1"
                     /translation="MKRNAKTIIAGMIALAISHTALADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            51864..52790
                     /gene="livH"
                     /locus_tag="N7C74_000325"
     CDS             51864..52790
                     /gene="livH"
                     /locus_tag="N7C74_000325"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HGU2430270.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            52787..54064
                     /gene="livM"
                     /locus_tag="N7C74_000326"
     CDS             52787..54064
                     /gene="livM"
                     /locus_tag="N7C74_000326"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HGU2430271.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            54061..54828
                     /gene="livG"
                     /locus_tag="N7C74_000327"
     CDS             54061..54828
                     /gene="livG"
                     /locus_tag="N7C74_000327"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HGU2430272.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            54830..55543
                     /gene="livF"
                     /locus_tag="N7C74_000328"
     CDS             54830..55543
                     /gene="livF"
                     /locus_tag="N7C74_000328"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HGU2430273.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            55662..56408
                     /locus_tag="N7C74_000329"
     CDS             55662..56408
                     /locus_tag="N7C74_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGU2430274.1"
                     /translation="MHYAVVVMLELLCCIHAYRTGQERYWIFIIFCFPVIGCVAYFVI
                     VMLPETGADRHGHTLLMRLQDKLNPERHLRKLTEELAIAETNQNHYALANELARLGRY
                     HEAVPHYQQALSGIFAHEAAMMLSLAQAQFAIQEFAACQQTLEDVMRYNPDFQSADGH
                     LLFARTLAAQEKYADAESEFEVLISYYPGPQARIYYAEMLAKMSRLREANEQYVAVVD
                     TAKRSRPHYRKHHREWIKTANERLKQSVVQ"
     gene            56768..58084
                     /gene="ugpB"
                     /locus_tag="N7C74_000330"
     CDS             56768..58084
                     /gene="ugpB"
                     /locus_tag="N7C74_000330"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HGU2430275.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            58182..59069
                     /gene="ugpA"
                     /locus_tag="N7C74_000331"
     CDS             58182..59069
                     /gene="ugpA"
                     /locus_tag="N7C74_000331"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HGU2430276.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSRFVGLDNFVALFHDSYYIDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVMQFRYVESKVRYQ"
     gene            59066..59911
                     /gene="ugpE"
                     /locus_tag="N7C74_000332"
     CDS             59066..59911
                     /gene="ugpE"
                     /locus_tag="N7C74_000332"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HGU2430277.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            59913..60983
                     /gene="ugpC"
                     /locus_tag="N7C74_000333"
     CDS             59913..60983
                     /gene="ugpC"
                     /locus_tag="N7C74_000333"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HGU2430278.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDICINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIALPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            60980..61723
                     /gene="ugpQ"
                     /locus_tag="N7C74_000334"
     CDS             60980..61723
                     /gene="ugpQ"
                     /locus_tag="N7C74_000334"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HGU2430279.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYGKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALSARELWAGMTPPLLSSFEIDALEAAQQAV
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(61710..62150)
                     /gene="yhhA"
                     /locus_tag="N7C74_000335"
     CDS             complement(61710..62150)
                     /gene="yhhA"
                     /locus_tag="N7C74_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HGU2430280.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPGTPQPDIPLKTIGP"
     gene            62270..64015
                     /gene="ggt"
                     /locus_tag="N7C74_000336"
     CDS             62270..64015
                     /gene="ggt"
                     /locus_tag="N7C74_000336"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HGU2430281.1"
                     /translation="MIKPTFLRRVAIAALFTGSCFSTVAAPPAPSPVSYGVEEDVFHP
                     VRAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLI
                     RSKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALD
                     KYGTMPLNKVVQPAFKLARDGFIVNDELADDLKTYGSEVLPNHENSKAIFWKEGEPLK
                     KGDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVER
                     TPISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYA
                     YADRSEYLGDPDFVKVPWQALTNKAYAKSIAEQIDINKAKPSSEIRPGKLAPYESNQT
                     THYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGG
                     DANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSLIITTVLQMVVNSIDYGMNVAE
                     ATNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELY
                     CASDPRSVDDLTAGY"
BASE COUNT        15074 a        16656 c        17554 g        14856 t
ORIGIN      
        1 attatcagtt tattgtgacg attaatagca aaaattacgg ttgccctctt gataataccg
       61 atattaattg gtgcggcatt gtttatttac ttaataatat gacagtaaat gataatgcta
      121 atgatgttgc agttactgag tcgtacaaac ccatctggaa ctggaaaata tcgcaataca
      181 atgtatctga tattaaattc gaaacaatta taaaacccca gttcgctgac agaatctatt
      241 tctccaattg ttcacctgtt gatcccacaa gtaccagacc aacctatttt ggtgataccg
      301 atggctcagt tggtgcagtt ttgtacgcgc tttttgcaac gggacatttg ggaatcatgg
      361 ctgaaggaga aaatttcctt agccagttgc ttaatataga agatgaagtt ctcaatgtat
      421 tgctaaggga gaactttaat gagcaagttg atactaatgt taatacaata atcagtatcc
      481 tgaacagaag agacattatt ttagaaagtc ttcagcctta tttagttatt aataaagacg
      541 cagtgacgcc atgtacgttt ttgggtgacc aaaccggcga tcgatttagt aatatctgtg
      601 gcgatcaatt tatcatcgat ttgttaaaac gcataatgag catcaatgat aacgttcatg
      661 tattagctgg aaatcatgaa acgaattgca atggtaacta tatgcagaac ttcacgcgta
      721 tgaaaccact tgatgaggat acctacgacg ggataaaaga ttatcctgta tgtttctatg
      781 actccaaata caaaataatg gcaaatcatc atgggataac ttttgatgat cagcgaaaac
      841 gttacattat aggaccgata acggtctcta ttgatgaaat gaccaatgct ctcgatccgg
      901 tcgaattagc ggcaattatt aataaaaagc atcatactat aattaatagt aaaaagttta
      961 aaactagccg ggctatctct tgtcgatcct ttaatcgtta tttttctgtt tcgacagatt
     1021 acagacctaa acttgaagca ctattagcat gctcacaaat gttaggtata aatcaagttg
     1081 ttgcacataa cggtaatggt ggaagagaat gcataggtga aacggggaca gttttaggtc
     1141 ttaacgcccg tgacagtaaa catacgggaa gaatgttcag catgcataat tgccaaatta
     1201 accctagtgc ggggcctgag ataaccactc cctggaagtc ttaccaacat gaaaaaaaca
     1261 gaaatggact tatgcctttg attagaaggc gcacaatgtt acagctctga accatagaca
     1321 acatgaatat taatgttact atctggttga gactgttgat aaaacgtaat aaggaatgct
     1381 ctgttcctgc aactgaacag gcgtgaaacg ctatccaaca ggatggatac cgttttgtca
     1441 gccagtttta tagatcacac ttatgagtat gcgtgtttca tttacggcca ggaatgctga
     1501 tgatgacaac attactaccc gttttcacta agccttcccc gttggcgctc aatgctctgc
     1561 gcgctggtcg tatttgtcgt ttccttctta tcccggatgg aagaatccgc tgaacgtcgt
     1621 tcggcggcag ttgtaacacg cgtcgtttgc ttacagggat tatggcgggt cgattatgga
     1681 taagagtaag cgccatctgg cgtggtgggg tgtcgggtta ctggcggtgg ctgctatcgt
     1741 agcgtggtgg ctgttgcgcc cggcaggtgt gccggaaggc tttgccgtca gcaatgggcg
     1801 cattgaagcg acggaagtgg atatcgccag caaaattgcc gggcgtatcg acaccattct
     1861 ggtgaaagaa ggccagtttg ttcgcgaagg cgaagtgctg gcgaagatgg atactcgcgt
     1921 gttgcaggag cagcgactgg aagccatcgc gcaaatcaaa gaggcacaaa gcgccgttgc
     1981 cgccgcgcag gctttgctgg agcaacgaca aagcgaaact cgcgccgcac agtcgctggt
     2041 taatcaacgc caggccgaac tggactccgt tgccaaacgt catacgcgtt cccgctcgct
     2101 cgcccaacga ggggctattt ctgcgcaaca gctggatgac gatcgcgccg ccgctgagag
     2161 cgcccgagct gcgctggaat cagcaaaagc ccaggtttcg gcttctaaag ccgctataga
     2221 agcggcacgc accaatatca ttcaggcgca aacccgcgtc gaagccgcac aagccactga
     2281 acggcgcatt gccgcagata tcgatgacag cgaactgaaa gccccgcgtg acggacgcgt
     2341 gcagtatcgg gttgccgagc caggggaagt gctggcggca ggcggtcggg tgctgaatat
     2401 ggtcgatctc agcgacgtct atatgacctt cttcctgcca accgaacagg cgggcacgct
     2461 gaaacttggc ggtgaagccc ggctgatcct cgatgccgcg ccagatctgc gtattcctgc
     2521 aaccatcagt tttgtcgcca gtgtcgccca gttcacgcca aaaaccgtcg aaaccagcga
     2581 tgaacggctg aaactgatgt tccgcgtcaa agcgcgtatc ccaccggaat tactccagca
     2641 gcatctggaa tatgtcaaaa cgggcttgcc gggcgtagcg tgggtgcggg tgaatgaaga
     2701 acttccgtgg cctgacgacc tcgtggtgag gttgccgcaa tgacgcatct ggaactggtt
     2761 cccgtcccgc ctgtcgcgca actggcgggc gtgagccagc attatggaaa aaccgttgcg
     2821 ctgaacaata tcacactcga tattccggcc cgctgtatgg tcgggctgat tggtccggat
     2881 ggcgtcggga agtcgagttt attgtcattg atttccggtg ctcgtgtcat tgagcagggc
     2941 aatgtgatcg tgctgggcgg cgatatgcgc gacccgaagc atcgccgcga cgtctgcccg
     3001 cgcatcgcct ggatgccgca ggggctgggc aaaaacctct accacacctt gtcggtgtat
     3061 gaaaacgtcg attttttcgc tcgcctgttc ggtcacgaca aagcggagcg ggaagtgcga
     3121 atcaatgagc tgctgaccag caccgggtta gcaccgtttc gcgatcgtcc ggcagggaaa
     3181 ctctccggcg ggatgaagca aaaactgggg ctgtgctgtg cgttaatcca cgacccggaa
     3241 ctgttgattc ttgatgagcc tacgacgggg gttgacccgc tctcccgcgc ccagttctgg
     3301 gatctgatcg acagtattcg ccagcggcag agcaatatga gcgttctggt cgccacggcc
     3361 tatatggaag aggcggaacg cttcgactgg ttggtggcga tgaatgccgg agaagtgttg
     3421 gcaactggca gcgccgaaga gctgcggcag caaacgcaaa gcgccacgct ggaagaagca
     3481 tttataaatc tgttaccgca agcgcaacgc caggcgcatc aggcggtagt gatcccacca
     3541 tatcaacctg aaaacgcaga gattgccatc gaagcgcgcg atctgaccat gcgttttggt
     3601 tccttcgttg ccgttgatca cgtcaatttc cgcattccac gcggggagat ttttggtttt
     3661 cttggttcga acggctgcgg taaatccacc accatgaaaa tgcttaccgg attgctgccc
     3721 gccagcgaag gtgaggcgtg gctgttcggg caaccggttg atccaaaaga tatcgatact
     3781 cgccgtcggg tgggctatat gtcgcaggcg ttttcgctct ataacgagct caccgtgcgg
     3841 caaaaccttg agttacatgc ccgtttgttt cacatcccgg aagcggaaat tcccgcaaga
     3901 gtggctgaaa tgagcgagcg ttttaagctc aacgacgttg aagatgttct gccggagtca
     3961 ttgccgctcg gcattcgcca gcggctttcg ctggcggtgg cggtgattca tcgcccggaa
     4021 atgttaatcc tcgatgaacc tacttctggc gtcgatccgg tggcgaggga tatgttctgg
     4081 cagttgatgg tcgatctctc gcgccaggac aaagtgacta tcttcatctc cacccacttt
     4141 atgaacgaag cggaacgctg cgaccgtatc tctctgatgc acgccggaaa agtgctcgcc
     4201 agcggtacac cgcaggaact ggttgagaaa cgcggagccg ccagtctgga agaggcattt
     4261 atcgcctatt tgcaggaagc ggcaggacag agcaacgaag ccgaagcgtc tcccgtgata
     4321 cacgacacca cacacgcgcc gcgccaggga tttagcctgc gccgtctgtt tagctacagc
     4381 cgccgcgaag cgctggaact gcgacgcgat ccggtacgtt cgacgctggc gctgatggga
     4441 acagtgatcc tgatgctgat aatgggttac ggcatcagta tggatgtgga aaacctgcgc
     4501 tttgcggtgc ttgaccgcga ccagaccgtc agtagccagg cgtggacgct caatctctcc
     4561 ggttcccgtt actttatcga acagccgctg cttaccagtt atgacgagct tgatcgtcgg
     4621 atgcgtgcgg gcgatatcac ggtggcaatt gagatcccgc ccaatttcgg gcgcgatatc
     4681 gcgcgtggta cgcctgtgga actcggtgtc tggatcgacg gtgcgatgcc gagccgcgcc
     4741 gaaactgtaa aaggttacgt gcaggccatg caccagagct ggctacagga tgtggcgagc
     4801 cgacaatcca caccctccag ccaaagcggg ctgatgaata ttgagacgcg ttatcgctat
     4861 aacccggacg taaaaagcct gccagcgatt gttccggcgg tgatcccgct tctgctgatg
     4921 atgatcccgt caatgctaag cgcccttagc gtggtgcggg aaaaagagct agggtcgatt
     4981 atcaaccttt acgtgacccc caccacccgt agcgaatttt tgcttggcaa acagctgccg
     5041 tacatcgcgc tggggatgct gaactttttc ctgctctgcg gcctgtcggt gtttgtgttt
     5101 ggcgtaccgc ataaaggcag tttcctgacg ctcaccctgg cggcgctgct gtatatcatc
     5161 atcgccaccg gaatggggct gctgatctcc acctttatga aaagccagat tgccgccatt
     5221 ttcggtacgg cgattatcac gttgatcccg gcgacacagt tttccgggat gatcgatccg
     5281 gtagcttcgc tggaagggcc gggacgttgg atcggcgagg tttacccgac cagtcatttt
     5341 ctgactatcg cccgcgggac gttctcgaaa gcgctggatc tgactgattt gtggcaactt
     5401 tttatcccgt tgctgatagc cataccgctg gtgatgggct taagcatcct gctgctgaaa
     5461 aaacaggagg gatgatgcgc catttacgca atatttttaa tctgggtatc aaagagttgc
     5521 gcagtctact cggtgataaa gcgatgctga cattgattgt cttctcgttt acggtgtcgg
     5581 tgtattcgtc agcgaccgtc acgccaggat cgttgaacct ggcaccgatc gccattgccg
     5641 atatggatca atcgcagtta tcgaaccgga tcgttaacag cttctatcgc ccgtggtttt
     5701 tgccaccgga gatgatcacc gccgatgaga tggatgccgg actggacgcc gggcgttata
     5761 cctttgcgat aaatattccg cctaattttc agcgtgatgt cctcgccgga cgccagccag
     5821 atattcaggt gaacgtcgat gccacgcgca tgagccaggc atttaccggc aatgggtata
     5881 tccagaatat tatcaacggt gaagtgaaca gctttgtcgc gcgctaccgt gataacagcg
     5941 aaccgttggt atcgctggaa acccggatgc gctttaaccc gaaccttgat cccgcgtggt
     6001 ttggcggggt gatggcgatc atcaacaaca ttaccatgct ggcgattgta ttgaccggat
     6061 cggcgctgat ccgcgagcgt gaacacggca cggtggaaca cttactggtg atgccgataa
     6121 cgccgtttga gatcatgatg gcgaagatct ggtcgatggg gttggtggtg ctggtggtat
     6181 cgggattatc actggtgctg atggtaaaag gcgtgttggg cgtaccgatt gaaggctcga
     6241 tcccgctgtt tatgctgggc gtggcgctta gtctgtttgc caccacgtca atcggcattt
     6301 ttatggggac aatagcgcgt tcaatgccgc aactggggct gctggtgatt ctggtgctgc
     6361 tgccgctgca aatgctttcc ggtggttcca cgccgcgcga aagtatgccg cagatggtgc
     6421 aggacattat gctgatcatg ccgacgacac actttgttag cctcgcgcag gccattctct
     6481 accgtggtgc cggattcgaa atcgtctggc cgcagttcct gacgctgatg gcaattggcg
     6541 gcgcgttttt caccattgcg ctgctgcgat tcaggaagac gattgggaca atggcgtaag
     6601 gaagggaaac ttgacttcat actgtgatgt catactattg atgtcataaa gtgaagtcat
     6661 aaggacatgc catgagaacc aaggtacagg ctttacggaa gaaacaaaaa aatacattgg
     6721 atcaaatatt taaaactcct gttcctcaag ggatcaagtg gtctgatata gagtcactgg
     6781 ttaaagcatt aggcggagaa attaaggaag gaagaggttc gcgttgtaag ttcatactaa
     6841 atatgagcgt tgcgtgtttc catcggcctc atccgtcgcc agataccgat aaaggcgctg
     6901 tagaaagcgt gcgtgactgg ttactaagta taggggtaaa accatgatca aactgaagac
     6961 gccaaactct atggagattg ccgggcaacc tgctgttatc acttacgtgc cagaactgaa
     7021 tgcatttcgt ggcaaattcc tgggcttgtc aggttattgt gattttgtct ctgacagcat
     7081 tcaggggctg caaaaagaag gggaactttc cctgcgagag tatcttgagg attgtaaagc
     7141 tgcgggtatt gagccgtatg cgcgtacaga aaagattaag acctttacgt tacgctatcc
     7201 agaatcgctg agtgaacgcc ttaataatgc ggctgcgcag caacaagtct ctgttaatac
     7261 ctatattatt gaaacactga atgagcgttt gaatcattta taacttatag cggcccaaca
     7321 atttctgttg cgccgctata tttttgtggc tcttaagatg cctttctaac caaccgatgc
     7381 ccctcattca tcatcaggat tttgctatac acatcttcct cgacggcgtt ttttgctgat
     7441 gccatgactc gtgccagatt cgcctcgttg tgattattca catacgcttt gccgacggcg
     7501 gtgataaacg ctttgcgcag atcgctggcg atatttactt tagcgacgcg ataattaaca
     7561 aagctgcgta atatctccgg ggcgataccg gagccgccgt gaataaccaa cggcaccgga
     7621 cagacttcgg caatgcgctt cagcaggggg atatcgatac gcggtatatc gtccaggcca
     7681 tgaacgttgc caatagaaac tgccagcatg tcgcaaccgg tacgctcgac aaaggtcttc
     7741 accttttccg gttccgtttt gcaatcggct tcgctgacgt gatcatcttc tttgccgaga
     7801 atcgccccca gctccgcttc aaccggtacg ccataggatt tacagaaatc gacagcttcc
     7861 tgggtgaaag cgatattctc ttcaaaagag aatgcggcac cgtcaatcat gaccgaggta
     7921 aagcccgcgc gcaccgcctg tttaacatcc tcctgggttt tcccgtgatc cagatgaaga
     7981 ctgaccggaa catccatttt atcggcgtga cgtttcacga tttcataaat gtaatcgtat
     8041 cccgagagtt gtgcattggt tggcgcaatc tgaatgaagt tgggtaatcc cgaacgctca
     8101 atagcgttta aaattgaaat tgtcgtctcc agattggtgg tattaaatgc gcccgctaat
     8161 acccgttttt cctgaatgcg gtcgagcagt atccgtccat taactaatgg catattgaaa
     8221 acctcatctt ttaatatcag ttaattattc agcttcaccg aagcggtcgc gaaataagtc
     8281 ggtgagagtc tgtatggcct cgttttcatc atcaccgtcg cagcgtattt ccaccgtttt
     8341 tccgcctttt attccggcgc gcataatatt catcatgctg gtggcattaa tatcattacc
     8401 ctcatagtgc agcgtcactt tacagctata ttttttggcg gctttcgcca gacaggccgc
     8461 cggacgtgca tgcaggccag tactgtttct gacctccacg gttttattca acatagtgtt
     8521 cctctcgatg atgtaatggc atcacgcctt cttgttgtaa tttcaactga taccagcttt
     8581 tccatttgtg ctgataacgc tgtaattgat ggtatctcgc ccgattcggt aacaaacgtt
     8641 gggctatagc gactggcggc ggtggaatat ccggtgcggc ggttaataat gctccgacag
     8701 aaaccgcctc gttaaatgca attgcttcaa ccggacgctg taaaatatcc gcctttaatt
     8761 gcagccaaag aggattatgg gttgccggac cgatcacttt taacgcgctg gtgccaggca
     8821 cgttaaaaca ggcgaagcag ttggcaaatt ccatggttaa tcccagtgat acgctggcaa
     8881 ttaacatgtc gatacttaac gtatcgccaa gcccataaaa taatccacgg gtatggcgat
     8941 ttttataggg cgaacccgaa ccgcgaagat gtggaataaa gacgggaatg tcatcgagca
     9001 accaattccc cgccagataa tcagcatgtc cgcgagtaag tgatgcggcg atctcctcat
     9061 cggttagccg gaacgtgttg cgcagccact ccagcgcgaa accgcccacg ggcagcgacg
     9121 caaacagggt gaactggttg gcaagtgagt aacagccgtt tgccagcttg tcacgtttgg
     9181 cctgttcatc cagcgtcggt tgtgtatcca gttgcagaat gccttccgtg gtccccgtcg
     9241 agttaaggat atcgcctggc atcatctgca acgctcgcgc acccaccata tggtcatgtc
     9301 cggccagcgt cacgctgacc ggttgcgaga acccaagcgt cttgcaaagt gactcgctga
     9361 cccatccggc gtgctcgcct ggctgcacca atggcgcaaa tgccgtacag ggaacgtgta
     9421 acaacgccgc cgctttcgct gaccattcgc ctttgcgcac gtccagacac agcgtgcggc
     9481 tggctaaggt gatatccgtg tgctgttcgc cagtcagccg ccagagcagc acttccggcg
     9541 catgtagcca gcgcaggccg cggcgattac gcagcgggta atgttccagc aaccagcgca
     9601 ttttgaaggc agagtaattg ctgtgtagtg gtagaccgca gatgtcataa agtgccgcac
     9661 tgtctgcctc gctaagcgtt gccagatact cttcaccgcg acggtcatac catgccagca
     9721 ttggcgtcag gatctcgcca tgctcgtcaa ggaacacgcc tgattcgcca aaactggcaa
     9781 tgctgatccg tctgactggc agcggcgaag cggcgttcag ttgcgctatc gcctggcgga
     9841 cctcctgcca gagggcgtcg atatcgaaat cgacatcgcc ctgtggggag atctgctttg
     9901 ccgtcacgaa tttatgcgcg cccaacgtcg ttgcgtccag gcaggaaaag caggtgactt
     9961 tgcaattggt ggtgccaata tcgataacga tagctgcgct gttatcaggc ataaaccgtg
    10021 ctccgcgtca ttcggaaaat gccatacagt accagggcga taaccggcac cagaccaatg
    10081 gcgatgagat tgccgttgaa gatttccagc agccagaagc ggaacgggtt accgccatca
    10141 agaaaactgg aaacctggcc gctggagcct tcggcaaatt tgaagttaac gtctttcgcc
    10201 attccggtaa taaacggcgc gattttggta gcaatccata ggtcggcaat aatcaccggg
    10261 atcgccgcca gaacggtgcg gaaaatattg ccacggctgg ctaaagcaat catcgacgcc
    10321 attaccgcca ggttggcaag gtcgccaagc ggcaggactt cattacccgg aaggacaaac
    10381 gcgataaaga ccgagattgg cgttagcagc agggcggtgg agataatcgc cggattacct
    10441 acggcaacgg cgatatccag gccgatatag agatcgtcac ggtcagggta tttggcattg
    10501 agatactttt taatagcttc agacagcggt aataaacctt ccatcaggat gcgtaccatg
    10561 cgtggcagga tgaacatcac accgccaatg ctgatgccaa gcagcaatac ttttttgaaa
    10621 tcgtatccgg caattacgcc gagcagaatg cccagaatag tgccaaccat catcggttcg
    10681 ccgaagatgc caaacttttt ctgtacggtt tccgggtcga tatgaatacg attgaggcca
    10741 gggatatggt cgatgatttt gtcgaccagc agaccgaccg ggaagaacac caccgaagag
    10801 agcgtcggca atgagatccc ttccagtcca aagtattttt gtaccagtgg cgcagaccag
    10861 tcggcgagtt taagtactac caccgcagca atcgccgccg ccagcaaacc aaagaacagg
    10921 ctgccggtgc tgtaataaac caacgtacca gcaagggcaa aatgccagta gttccacata
    10981 tctacgtcaa cggtacgggt tttatttaac gccagcatcg ccacgttaat cagaatggtc
    11041 aggggaataa caaacggggc aattggcgaa ccccatgtaa tagcggaaag cggcggccag
    11101 cctaaatcta ccaccggtaa attaattccg gtgcgttcaa ccatcgcctg ggccgctggc
    11161 ccgacattgc tggtcagaat atcgaaaatg gcataaatac cgacaaaacc aatgccgact
    11221 gttaatgacg aacgtaaggc tttagcgggt tttactttaa aacagagagc aagaataaat
    11281 aacaccaacg gcaacattac cgtcgggcca aatcccaata tatattgcac aatattatag
    11341 agggtatgcg cgatatcgtt cataataaac ctctttttca ggcgagagcg cacctcgcgt
    11401 tagcgcctga tggagtgggt tgaaattaat tattattaat tactttttaa ggatagaaac
    11461 aatttgcgcc agtacttttt cttcgccaat accggtaata atcggcaagc cgttcaccac
    11521 cgggacgtcg agttcataag gcactttagt tgttgaaact accagtgcta cgccgtcagt
    11581 atttccaggt aaagaggcaa cgttggtttg ggaataattg acgttaagac cttgctcttt
    11641 gcaatattcc atgacttttt ctgttaccgc cgtggatgtg gcaatacccg tggcgcagac
    11701 aaataaaatg gtttgactca tatttcacct cgttctttca ttttgtttaa gattactgtg
    11761 tgtatttctg ttgtttccat cgcaagcagg gcttgcataa agggcgtatc ctgaatcatt
    11821 tccataatcc agcccagggc atttaattgt ttattgcttt cgcttaaggc gagcaaaaaa
    11881 atcaccctga caggtacggg gtcaggatcg ccgcccatat cggcaaactc taccggttcc
    11941 ggcaggatgc cgacggcaat tgcattgtgc ctgacatgtt tatggtccgt gtgcggaata
    12001 gcgacgcacg gtggttcggt aggcagcccc gttgggtacg tcgcctcacg tttcagtacg
    12061 tgatcggcaa aatcagggac gaccagctcg gcggcaacca acggttgcgc taactggcgg
    12121 atgatctgtt caacgcttaa ccctgccgga agtcgttgaa tataattttc ctgaaaatgt
    12181 atgccttgca ttttattcct cttaaaaaag tgtacttaac tcacttcccg ccgttgcagt
    12241 acggtgccaa gataatattt atttcctttt aaccagacgt tgccaaactc aatgggtaat
    12301 cctcgggaga aaaagactaa ctgctccaga tgcaaaacgg gtgcgtcttc actgatatct
    12361 aaaaaatgac cacgttcatt accaattaat cgcgcggcat agcgactttc cgagtaacga
    12421 atttttcttt gcgacaaact ttctattgtt ggaaataaat tgtgttgatt aaaatcgatt
    12481 tcagcaatac cggggcatag ctcaatattg atacggttct ctatcagcat cgctttttca
    12541 tcaccaattg agcgtaagcg ttcaaggtaa agaatatcct gaccgggcgt tattcttaat
    12601 ttctctgcca cataacgatt agccgattca atccgtgacg taataacttc agtggtaaaa
    12661 tgtattttct ggctttccag cgattccgcg aatgacaata atccttcacc taacggatat
    12721 gccacgttct cttttttgac aaatgttccc ttaccctgga cctgtattaa cacgccttct
    12781 tttaccagtt gggcgaccgc ttttttaacg gttccccgac tcacgtccag catatccata
    12841 atctggtatt ccgaaggaat gcgatcgtct tccaccagat ctccggtata aatactttct
    12901 cttatccact ccaccacttg ttgatagagc ggtatttgtg acgacttact taatgatttc
    12961 atggcgcgca cctgaacatt tacagttgtc tatggacaat catggacaac ttcaagcgta
    13021 tagtaggtga acgggagaaa aagaggatcg aagcgtctca cattttgagg gaccaaatcg
    13081 tgatcaaaaa aatgtgacgt gagtcgatat ttgtcagaag tttactgcgc cgtaaggaaa
    13141 taacggcgcg ttggtaggtg ttaatcttcc ttcggcaggc actgcaaatg cccgtgccgc
    13201 acgccgcgct gggcgataac gtcatcggca aaatgctgca cgtcacccat gtcacctttc
    13261 aacacggcga tttccagaca gtcgtcgtgg ttgatgtgca catgtagcgt ggcgacggag
    13321 aggtcgtggt gatgatgctg ggtggaaaca atacggctgg ctaagtcgcg tttttcgtgt
    13381 tcatacacat acgacagcac cgcgaaacct tgtgtgccgt gctgctgggt agcttcctgc
    13441 gccagggcgc tacgcagaat atcgcggata gcttcggaac ggttgttata accacgacgc
    13501 tggctcaggc tgtccagcgt ctccagtaaa tcgtcatcaa gcgtgatggt gactcgttgc
    13561 atttgcgtta aaccttttct gtggcgcgac ggcgcacggg gaatgcgggt aatatcgcgt
    13621 tttgtagcac acgtccggcg tcagaggaaa aggttaattt ctctcccacc gcctgggttt
    13681 cgacgatttg tccgttgtcc atcaccatca ctcgctggca aaagcgttcc accaggcgta
    13741 agtcatgagt gatgaacagg caggcagtgc caaactgttg ttgtagcttt ttcagcaggc
    13801 gaatgacgcc cgcctgtaac acgagatcaa ggttagaaac ggcttcatcc agaatcagca
    13861 atttcggttc gaccgccagc gcgcgtgcca ggcagacgcg ctggagctgg ccgccgctta
    13921 actgcggtgg gcgcttgtcg agcacgctgt cgtcgagatc gaccgcgtgc agcatttcgc
    13981 tggcacgcgc cagttgttcg gattttttca gcgatagcag gtggcgcatc ggttcccgca
    14041 ggatctcgcg cacggttttg cgcggattca cggcgctgat ggagtcctga aataccatct
    14101 gaatatcgcg gcggaacgct ttacgctggg cgcgattgag ctttgccaac gattcgccac
    14161 gccagctaat atttccctgt gagggcgatt ctaagcctac cagtagccgc gagagagtgc
    14221 tttttccgca gccgctgcgt cccagcaggg cgacggtttc accgcttttc agggtcaggg
    14281 aaacgttatt cagcaccgcc tggtgctgat gttttccgct aaacccaccg tgggcatagt
    14341 gatgggaaag gtcggagacg ttaagtaaag tcatgatgcc agctccatac cgtagagggc
    14401 gagatgagcg gaaaccaggc tgcgcgttac cgcatgtttg ggggcgttaa acagcgtttc
    14461 gacatcgccc tgttcgacaa tcttaccgtc agacattacc gctacgtcat ccgccagacg
    14521 cgccaccacg cccatatcat gggtgaccag cagcatcccc ggcgcctgtt tttgcataat
    14581 gctttccagc agatcgagaa tgcgtgcctg tgctaccacg tcgaggtcgg tggttggttc
    14641 atcggcgatg ataaacggtg attcgcaaag caccgccatc gcaatcatca tgcgctgcaa
    14701 catgccgccg ctcatctcga acgggtacag cttcagcacg cgtgcggcgt tttccagccc
    14761 caccgcttct atggcagcgg taagcgtagc gtcatcggcg ggtttcccca gcgccaggca
    14821 cgtctcccgc gcgtgggtgt gcatggtgtg cagtggatta aaggcgctgc gcgggttctg
    14881 catgatggtg gcaattttga tgccgcgcag ggcgcaggga gaaaccggtt tgccatcggc
    14941 taaaatttcc cccgccgtct ggcgaacgcc tgcgggcaga attcccagcg tcgcggcgca
    15001 ggttaatgat ttcccgctgc cgctaccgcc gactaacgcc agcacgcgcc cgcgtttcag
    15061 ggttaacgac acgccgtgca ccagcggctg cgcggcctgt agcgcgatat tacgtagttc
    15121 aatctgttgc ggcattagtg tgcgtgctcc gtcaccagat gaggatccag atgatcgcgc
    15181 agtgcgtcac ccaccaggtt aaaggccatc acgctgataa acagcgccag ccccggccag
    15241 aacatttgca gcggctgggt ccagatatac tggcgcgcgt cgttaatcat cacgccccat
    15301 tcggcggtcg gcgcggtcac gccgaggccg aggaaggaca tcccggcaac gtgcagcatc
    15361 atatggccga tatccagcgt tgccagcacc agcagcgaag ggatcaccgc acctgccaga
    15421 tgatcaacaa acactcgcac atggcccgcg ccagaaagcc gtgatgccag cacaaactcg
    15481 cgctggcgca gtgaaatcac caggctgcgc accatgcgtg cataccacgc ccagtgcgac
    15541 agggcgatgg cgataattac gttggtcagc ccagtgccga gcacgccaac cataaagaat
    15601 gacagaatcg aggtcgggaa ggtcataaac atatcggcga cgcgcatggt agcctgatca
    15661 acgcgcccgc caatcagccc ggcgctgccg ccaataacca gccctaatgt cagcaccagc
    15721 agcaggcagg ccattaccga accgagcgac acacgggtcg ctgccatcag ccgcgagaaa
    15781 atatcgcgcc ctaagtgatc ggtgcccaac cagtgctgcg catccggcga aagcaggcgc
    15841 gacggcaaat caatcgcctg ggggtcatac ggcagccacc actggctggt gagcgcaatc
    15901 agcgccagca gggcgataat gatcagcgcc aggcgtaccg accagcggga agagaggaaa
    15961 aagttcacgc gtgcgctcct tcatgacgac gaatgcgtgg gtccagtgcg gcgttgagca
    16021 aatcgacaat caaattacag accacaaaaa ccaccaccat catcagcgta aagcactgga
    16081 tcaccggata gtcacggtta aaaatcgccg acaccgcata gcgcccgacg ccgggccagg
    16141 taaagatgtt ttcgataatc atcgtcccgc caatcagttc gccaatgtgc atccccacgg
    16201 cggtgatcat cggcagcgag gcattgcgca gaatatgacg acgctcggtc tgtttgtcat
    16261 tcaggccgcg tagccgcgcc caggtgacgt gacgctgacc ggcgacgtcc agcatactgg
    16321 cgcgcagcag ccgtgcgtta atcgccagcg acataaaggc aatggaaacc gcaggcaaaa
    16381 tgatgtgctg ccagccgccg tagcccatcg cgggtagcca ttgcagatac accgaaaacg
    16441 ccatcaccag taaaaatgcc agccagaagt tgggcatcga cacgccgaga aacgcgatga
    16501 aacgtacggc gaaatccggc agacggtcac gatggcgcgc cgcccagata ccgagcggta
    16561 cggaagtgag cagaattaat accagcgccg cacccgccag ttccagcgtg gcgggcagga
    16621 agttcagcat atcgtccagc accgggcgct gcgtggcaaa tgagatgccg aaatcaagat
    16681 gcagcgcctt ccacaaccag gtgccgtact gcacatacag cggctgatcc agtcccagca
    16741 tggtgcgggt agaggccagc atctccggcg tcggcggcag gttagacaga cgcaaataat
    16801 cgagcgccgg gtcgccggta ccgaggcgca gcatcagaaa aatgatcacc gaggcggcga
    16861 gcaccatcgg gatcagcagc agaaagcggc gtaatacgta acgcaacatt aaggtttcac
    16921 cggtttaatc tgttcgaacg gaatttcggt ggcgatcggc gcgtagggga tgttacccag
    16981 ctccggtttt gataccacca tcattgagat gtaactgata ggcagataaa ccgcctcgtc
    17041 atgcagacgg gtcagaatgt cgcgatacag tgcctgacgt tgtgcttcgt catgggtcgc
    17101 cagcacttcg ccgatctctt tatcaatcag cggcttgtcg gctaatcctt gctgtgcctg
    17161 gaagtcagcg tgcgacggta cgcgcattga actcaggaag gcgtgtggat cgtatggcgc
    17221 gccccaggtg cggtggaaaa tcatgccaaa acgaccgtcg cgctgacgcg catagatact
    17281 gctctcttct tcgccaatca gcgagacatc tgcgccaatc tggcgcatat cagcctgaat
    17341 gatttccgcc atcgatttgc ttaacgcatc ggtgccgatg aaggaaagtt caatgtgcag
    17401 cggctgacca tttttctcgc ggatgtcttt gcccgcaggc agcgtccaac cggctttttc
    17461 cagcaacgct ttcgctttct gcggatcgta ctggcgcggt ttcaggccga ggttggcgta
    17521 gggcacagaa ggggcaaaca gggtgtcggc gacctgctgg gtgccataca acgcgttatc
    17581 aatcagcgat tttttgttta ccgcgtaatt aagagcttca cgtactgcca gctcattggt
    17641 gggggctttg gcggtattaa gcgccagcat cacggtttcg atcggctgtg acagttgggt
    17701 atggtaagcc gggttctggc taaagcgggc gaaggtatcg agcggtaata acccttcgtt
    17761 tccgtacagc aggtcgatat cgccagtttc aaacgccacc gcgcgggtag tcgggtctgg
    17821 gatgacgtta aaagtgatct tcttaattgc cggcttttcg ccccagtagt tttcgttacg
    17881 gacgaagaca tcgtactgat tcagtttcga ttcctgcaaa acccacggtc cggtgccaat
    17941 cggcgcttta atcccgttca tggtttcatg gtttttaaac tgcgaggggg cgataaagcg
    18001 gaaagggcgc ggcagggcca gttcttgcag gaaaggatag taggcgcttt tcagggtgat
    18061 ttgcagctct gttttactga gtgctttaac atcaacaatc tggtttgcca gctccagcca
    18121 ggcgtgacgt tgacggttat cgagcactgc gcggaagttt tctgccgccg cctctgcatc
    18181 aaacggttcg ccgttagaga atttcacgtc atcacgcagg gtgaaggtcc aggttttacc
    18241 atcttctgaa tgagtccagc tttttgccag ccacgggatc accgaaccgt ctgcctgata
    18301 tttcaccaat ggttcataaa ccatgctctg ggcgaacatc tggttaggcg tgtaaaggtg
    18361 cgggtttagt ggcccgacat tcaccggcca ggcggtggtg atttcatctg gtgcagcggc
    18421 atggacgata aaagacgcac aagccagcag cgcaaataga gtgcggcgga gtgtggagag
    18481 catggtaacc ccaatggatt aaaatagatg gcggaaataa gtatgacgat tttaagtatt
    18541 cgtcatactg attacctgtt ctggatcaat agatgggcag tcgggtgggc ggatcagtta
    18601 actgaatcga tccattgcac actgtcggcg gtgagcgtaa agggagtagg ggtgcagatc
    18661 gccaggctta aattttctag ctgacaatgg ctgactgaca gcgaggagtg ataggtgctg
    18721 tctacgctga cgatttgcca ggcgctgcca ccacgctgtt taacgatggc ttctttgcgc
    18781 gtccagatgc gccagaacat ttccagttgc tgatcaggat gcacggcgtc catctcagca
    18841 tgttccccga ggctgaacac ggcgttcgcc agccagcgcc agttggcgcg cgggcgaatc
    18901 acttcgatat cgcagccgac ttcgccttca tcactcaaca gcagggcgat atcgtcaccg
    18961 ctatggctta agttgaacca tagcggcgtt tccggcgcaa atgcaggttt gccttgttcg
    19021 ccatagatga tctccggtag cggggaaagc gtgtgcgaaa gcaatgcacg ccccgccagc
    19081 cagcgttcgc gtcgtggacc ttgcggtgct tgctcgcgta aacccggtgg cagtggagct
    19141 gcgcttaagg tcgaaacttt ccccagaact atccgataca tatcagggcc aacgtttaat
    19201 gatgatggat gtattaatgc cgccaaaggc aaagttgtta ctctgcaaaa actcacaatc
    19261 aaccttgcgg gcttcatgca tgatgtaatc cagcgcgcca caattcgggt ctggtttatt
    19321 caggtttagc gtaggcgcaa accaaccttc gcgcatcatt tgcagactca tccaggcttc
    19381 gagcgcgcca caggcaccaa gcgtatgacc aaaataactt ttcagcgagg agagcggcac
    19441 attatcaccg taaatggttg ctgtcgccag actttctgcc atatcaccgc gatcggtcgc
    19501 cgtaccatgc gctgaaatat agccgatatc ttgtgcgctt aacccggcga ttttcagcga
    19561 ttgctccata caatactgca tggtttcgcg ctgcggctgg gtaatatgtg ccgcgtcgca
    19621 gttagtcgca aagccgacga tttcaccgta aatggtcgcg ccgcgcgctt tggcgtgctc
    19681 cagttcttcg agaatgagcg tacctgcgcc ttcaccaatc accagcccgt cgcggttttc
    19741 atcgaacggt gaaggtgtgg ttttcggcgc atcattgtgc tggctggtgg cgaaaagcgt
    19801 atcaaatacc gctgcttctg aaggacataa ctcttcggca ccaccagcaa ccatcaccgt
    19861 ttgataaccg tggcgaatgg cttcccacgc ataaccgatt gcctggctgc cggaagtaca
    19921 ggcgctggag gttgggatca cccgcccgcg caggccgaaa aagaggccag tattaacggc
    19981 ggtggtgtgc ggcatcatct gcacataggt ggtgccagtg atgttattgg tgtgcttttc
    20041 cgtcagcatg gtggcgaact cgcttaccgg gccggtactg ccggtcgatg aaccgtacgc
    20101 aatgcccgtt tcgccgctgg ttaatatcgg atcgccaatc aaccctgcct gctctaacgc
    20161 cagctcgctg gcacgggtcg acatttgcga cacgcggccc atcgcacgga tacgcttgcg
    20221 ggtgtagtgc tccggtagcg tgaaatcatc aacaggcgcg ccaagcaggg tgtgcagccc
    20281 atcgtagacc tgccactccg gcattttgcg caccgcattt tcatatgcca gtaagcgtgc
    20341 agaaacgtcc tgccagtttt caccaaaggc agtcacgccg cccattcctg taatcactac
    20401 gcgacgtgtc ataacatccc tccgttgatg gaaataacct ggcgggtgac gtaacccgca
    20461 atatcagaca ttaaatagct ggcaagcccg gcaacttctt ccgcctggcc catgcgtttc
    20521 atggggatca tcgacattgc ctctttcagg gcgctctctt ccatttcgat catcccggta
    20581 tcaataagcc ccggcgcgat gcagttgacg gtgattttgc gcttcgcgag ttcgatagcc
    20641 agcgcttttg ttgcgccgat aattccggct ttggcagcgc tgtaattcac ctggccgcga
    20701 ttgcccatta cgccggaaac cgaggatagc gtgatgatgc gcccaccctg gcgtgcgccg
    20761 atcatcggca taatgcacgg ctgaatgacg ttgtagaaac tgtcgaggtt ggtgtggatc
    20821 accgcatccc agtcgtcatc gcttaaagcc ggaaaagcgg catcgcgggc gatcccggcg
    20881 ttactgacca cgccgtacca ggcaccgtgt tgcgcgattt cgtgctccag cacttcccga
    20941 cactgttcgc ggtttgcgac gtcgaaactg agcaaacgcc cgttaccccc attggcgaca
    21001 atggcattca gcgtttcctg agcgcctgtg gcgtcacggt gatagtgaac gccaatgtta
    21061 aagccgtctg ccgccagttg gcaggcgatg gcgcgaccaa tgcctttgct ggcaccagta
    21121 accagaactg aacgactcat caggacgctc cttgttgaaa cagggtggta agttcttccg
    21181 cggtcggctg gaaggtgtta atgcggcccg tcgcctcgcc gtcgttgatg ctgcactcaa
    21241 aactgccgaa gcgatcgtct tgcatcagca gtttgacggt aatggtcagt gtctggcctg
    21301 cgggcagaat gccagtggtg caaagcaatt cacgcgcgcc cagtatcatc cctaactcaa
    21361 tactgttttt gccctgctgg tggcgatgcc agccagacca gacgccgacg gtttgcgcca
    21421 tcagttcaag ggtaaaccag ccggggagat tgccgtccgg gtcgagaaac ggtgccagta
    21481 cgccactggg cgaaaccgtc acgcggcaca ccgcgctgtc atcgctgacg ctcaccacgt
    21541 cttccagcag taacatgggc gcgtcgtggg gcagataagc gccgggggat aaatagtggc
    21601 tcacgaaacc ctcccgagca gaatgctggc gttattgccg ccaaaggcga aggaattgga
    21661 caggatcacc ggacgtgcca gcggctgcgg tttttcgata atgccacacg gtggcagcgt
    21721 gggatcgcgc ggcgacaggc tgaagtcctg agcgggcagc ggtaaatcac gctgtaaaat
    21781 caacatacta attgccgcct cggtaatgcc cgctgtgccc agcgtgtgtc cggtcaaatg
    21841 tttggtggag ctgcacggta cgcgttcgcc aaacagggcg ttaaccacca ttgattcaat
    21901 ctgatcgttg agttgtgtgg cggtgccgtg caggttgata tagccgacgt catctggcgt
    21961 aagctgcgca tcggtcagcg cctgattaat agcgcggatc gctccttcgc cctgcggatg
    22021 cggggctgaa atatggtaag cgtcgctcga ttcaccaacg cccagcaacg cgatgggctg
    22081 cggttcccgg gtcagcagca ttagccccgc cccttcacca atggtgatac ctgcgcggtc
    22141 acgaccaaac ggctgacata aggttggcga cagcgattcg aggctgtgaa aaccgttaat
    22201 cggcatccgg ctcagggtgt ctgcgccacc cacaatggcg gcatcgacca gccccgcttc
    22261 gataagcctg cgcccgctga ttatcgctct ggcgctggaa gagcaggcgg ttgaaagggt
    22321 ataggccggg ccgtcgagcg ccagccagtg gctgagaaaa cgcgacggat cgccgagttc
    22381 ctgttgcgcg tagtgccatg tggtgctcgg ctgcccgtgg gtgcgcaggt taacgtgcgt
    22441 gtcgccttca tgcaggccgg aggtgctggt accaagcaca atcgcaatgc gctcgcggcc
    22501 atatttcgca atggcgtcat caacctgcgg ctggatctgc gccagcgccg ccagcaggat
    22561 ctgattgttg cgcgagcggt gcgcagcgaa tttttccggg ataagcggaa gctcgccatc
    22621 cacgccagcc aggaccgcct gcggatgccc ctgcaaccag cctgcgcgcg ggcgcatgcc
    22681 gggagcaaca ccgcgagtca ggttggttgc gatttcgtca aggttatttc ccagggcgtt
    22741 gatcatgccg acggcggaaa tataaatcat atcagtcacc taagtattga atggtgatgt
    22801 ggtatttaaa gacatgctgc tcaatgctga ttggcacgcg tttgcccttg cgattcagat
    22861 aggtgatttc cgtgaccagt ttgccgctgg cgttacgcag ctcgcgtttg tcaccgttgt
    22921 cgcgaagtgt ccatcctgcg ggaagttgcg gttgccaggc gctaatcggc cagtggctga
    22981 gcatcacgtc agccagtacc tgacttgcgg gcggtaactg tgggacgacg atggattgct
    23041 cggcgcgtag cccttgcgca tcgtaggtca ccagaaacag gcgaatgccg accgacgaca
    23101 gcccggcaag ggtgattttc tgatcgtcgg cgttaagcat cactaacagc gactgggttt
    23161 tgccgttgaa gctgccggtc aacagttgct gcgaattgac tgcgggtgaa atccctggtg
    23221 ccggcagcgt gatgcgcgtg ccaggttgca gccacgcctg cgggcggcct tcctgttctg
    23281 gttgcgagtg gctacagccg gtgaggataa gcgtagcggt cagggcgaac gctcgccaga
    23341 atgtggattt gatcattttt ttgttctctt tttatcgggc atagccagcg gcgaaagcag
    23401 gaaggcggtg aaaataccgc tcaccagcac aatgccaaaa ctgctgatgg cctgggtggc
    23461 gctgaatacc agcataccca gcgtcagcaa ggtggtgagc attgccagcg cgatcgccag
    23521 tagcgaagtt aacggtgtac cgcgcggatt actgaaaaac agcgtgtagt tgataccgat
    23581 gccaagcacc agcaccagcg ccagcagcga aaagagattc accgcctgcc cgctcatcgc
    23641 cagcaccgcc agaccacagc ccagcgaaag caccgaaggc accaggctga taagcccttt
    23701 gcgccagccg agacgggcca ctgcgccgca ggcaatcact gccagcgcca ccagcaacaa
    23761 gccggttaag acgtagcggt aaagtgcgaa caattcatca aaggtgcttt tgcgatcaac
    23821 ccaggcaatg ccgcaagggt aatatgtggc gatttcctgc atcaacgcgc tacttttaac
    23881 cccttcaacc ggcaccagta cgccgctttc gccgttttcc agcgtcagcc acagcagacg
    23941 ccagccttca ctggcggggc ttgccagcca ttcatcaacg ttcactggca tggcgttgag
    24001 atccgggttc actgccgtca gcccggcatt ttgcagcgct tttgttactg tcggggccgc
    24061 cgttttcagc agttgtaaat cttcctcctg ccgcgcaagg gagttcagcg gaatggtgcg
    24121 gtagttgctg ataagcccct cttttttcgc atactcaagt gaggcggtat atttctccag
    24181 tcgccgcaat gtttgctgtg gcgaatcgcc gtaaaccaca aaccattttt gatcgacgct
    24241 ctgcccggtc agggcggtaa tggctttttc ctgcgccaga atatgctgcg gtagcgcctg
    24301 taactgcgag atatcgtcat cgacgcgtag cattgacatc cccgccagcg aaaacagcgc
    24361 cagcgcgacg ggcagaccca gcgacagttt tttattacgc cgccacgctg ccagccagcg
    24421 tagcatcagc gccatcgccg gaaccggacg caccggcagg ccacggcaca gccacggatg
    24481 ccagaacagg acggtcagac aggaggcgct caacccgacg gcggcaaaaa tcgccatctg
    24541 gcgaatgccg gggaaggggg cgagcatcat aatcagatac gccgccacgg tggtgagcag
    24601 cgccagtagc aaggcattgc gtactttcgc caggctttgc cacggcgaaa cgtcgttgcc
    24661 gtgaaccatc cgctcggtga gataatagag cgtgtagtca gcggaaatgc cgataacgct
    24721 catgctcatc accagcgtca tcaggtgtaa ttcaccgaaa atcaataaag tggcgaccgt
    24781 tcccgccagc gcgccgatgc cgatggaaat cacgcaaagc agcaacgggc gtaaagagcg
    24841 gaacaccgcc acaatcagca atatcacccc cagcagcgta gccacgccca gggtggagat
    24901 atcctgcttc gcctgttggc tggcgtaatc gctgtaaaac accgtgccgc gcgagagcaa
    24961 ctgtgcctgc gggtaacgcg ttttcagatc cttttccagc gtattcaggg tcgtgatcag
    25021 ctggtgggtt tgctgcatat caaacgacga tcccgccagt tcgccgtgca gcagatacca
    25081 gtagttgccc tggggatcct gcgtcaccag ccaaccgtcc atcagccgca aacgctggcc
    25141 gtttttcgcc attgccagct gcgagccgcg cattaacatc agcggatcgt tttgcagctc
    25201 cttgccgctt acgccggaga atgcggaata aagctgggag aggatccact gtgcctgcgc
    25261 ttcgccgccg ttttgcaggc gggcgcgggt gttggggtca atcaggccgt tgcgatgctg
    25321 ccagaaaaac gctccccacg cctgctggct ggcggcatcc attggccctt taacgtcgcc
    25381 gagcgcagcg gatttttgca gcagcgtcag ccactcctga gcgaccaaag gattagcctc
    25441 tttaccgggg ctgaccagcc acaccagttg gcggtcaaga cgctgcataa agccgtcatt
    25501 cagcgccgga ggaatatcgc ccatcgtctg tttgggtaac atagccagca cgctactgtt
    25561 cagccgtgat tgcggcagca ggatcagcaa cgccaccgcc atgaccaggc agactagccc
    25621 ccataacagc gcggggcgtt tactgggcgg caaaacgttg gcgttcgtca tcggtcagtt
    25681 gcgctggcgt cagttgatgt tgggtaagag caatatcggt gcgatcgccc tgtttatcat
    25741 taagttgaat gctctccaga taggttttcc cggcgagatc gatggtgttg aaaattttat
    25801 ccagcggcgt ggtggtcggc gtcaggcgca gcgtccagcg gccttcgcct ttgtcagcaa
    25861 attcgacgcg gaagttttgt tccagcactt tgcgatcggc ctggaacaac gcgcgcagca
    25921 ggtggttaaa ctggaacatc tgcgggttgt tttctgccgt gatgatttgc ggcggctgac
    25981 cgttgatcac ctgcaccatg cgtttatcgt ccagtaatag ttgcatgggg aacggtgagg
    26041 tttgatccca caacagcccc tggtcgcggg cgatcaacat ctggccctga gatcgcagcg
    26101 gctgcggcag atctttaatc gtccgggttt gatcgaaatg ggcgcggatc accggttgtt
    26161 cggtaaagcg ttgctgaaga tcgtccaggg tcagggcgct cacaaacggg ctaatcagca
    26221 gcgccagcag cggtaaaaat ttcatggttt gactcccatg cgttcaaaca gaatatcggg
    26281 gctgacaaag cacagttcgc gactttgctc gtcgaccgcg acctgaatgg tgtatccggt
    26341 ggtggcgcgt ttgcccgttt cggcatcgaa aatctgatag ccaatccgca gacggttttc
    26401 gaactcttcg atatgcgcac gcacgcggat gcgttgctca aaggtgaggg cgtggcgata
    26461 tttcacccgc gcatcgacca ctggccacaa ataaccggac tctttcattt gccgatagcc
    26521 gtaattgaac tggttgagca gcgcctcgcg agccacttca aagtagcgaa aatagttacc
    26581 gtgccaggcc acgcccatca tatcgacgtc gtgaaaggga atggtcagct cgacttctgc
    26641 ggtaaatcgg ggatcgttca gcacccttta ctccttgtcc tgaatttccg gcagttgcca
    26701 gaaatcgaaa aaattaaacc agtcgagagg cgactggagc gcgtaatgtt ccagacgcgc
    26761 ggcgtaatga tcgatagcgt tttgcagcgc ctgttggcgt tcgccgcgcg gcagctgcag
    26821 tgggtcggca aagctttcgc agtgaatatg cagcttgccg tgctggcgca gggcgaaaat
    26881 cagattcacc gggcagcgca aaatagaggc gagaataaac ggcccctgtg ggaaaggcgc
    26941 aggctggccc ataaacgaac tccagcagac gcgccagtcg ccgccgcgtt gcgggttgac
    27001 ggcgatgcgg tcaccgacaa tcgccaccca ttcgccgtta tccagcttct ctttcagcag
    27061 gatggcggtt tctgggccga tatctgttac cggcatcagg ttaatgcctg cctgaggagc
    27121 catctcctgc attatctgtt taaagcgttg ggcgttttcg ctaaacacca gcgcgttaat
    27181 ggttttgtag ccctgaattt ttgccagcgc ccggcacact tccacatcgc caagatgcga
    27241 ggccagcagc aatttgccgc gcggatcgcg gacgtcaagc gctgcttctg cgcctggcgc
    27301 aaacagcaca tcacgccctg gttgtagctc gccgcgccag ctggcgattt tgtcgagcat
    27361 ggcattaccg aaacgtagaa agtgctgata gctgttgagt tttgccgcct gtggttgatg
    27421 ctgtcgtaca cgggcgagcc agtcttgcga cgctttgcgc gcacgagaag cagtgagcca
    27481 gtagacgccc accaccgggt aaagcagcgc gctaaacgcc gttcttccca gcagacgcca
    27541 gaccagcagc atcaggcgca ttccccataa tcctttcact tcactctgcc gcgcccagtg
    27601 gcaggaagag cggcgcatca gcagtgaagg aatacgcggc aacatgccga aaaacagacg
    27661 cgtgtgcatg agcgagatgc ggacgttatc tttcagggca tcaaaatgcg aaagcccgtc
    27721 cagtggatag gtgacgcggg tcggcacgaa atagctggta tttccctgcc agtagaggcg
    27781 caccatcact tcggtgtcga aatccatccg cttgccgatg gtggcgtgtt ttgccagttg
    27841 cagcgttggc gcaaccggat aaacgcgaaa accgcacatg ctgtctttca gttgcaggga
    27901 gagcgtttcg atccataccc agacgtgggt gacccagcgc ccgtaaagcc gtgagcgggg
    27961 gatggagtca tcgtaaattg gctggccgga gatcagtgcc gcaggttgtt gttcagcgag
    28021 agccagcagt ttagggatat cttcaatcgc gtgctgaccg tcagcatcca cctgcaccgc
    28081 atggctgaac cctgcgtctg ccgctgcctg taagccacgc atcaccgccg cgcctttgcc
    28141 tgcgttttct gccaggcgaa ttaaggtcac gccaggctgt tcggcaagca gactgtccag
    28201 ttgctgttgt gtggtggcat cgctgccgtc atccaccaca atacagggca gattaaatgg
    28261 cttaagacgc gccagcacgc ccggcatcat cgcgccgtgg ttgtagcagg gtatcaacac
    28321 gcagggagaa aagtttaccg acataggcgg atcttcccgc tactggcggt gtggcgggcg
    28381 tcaccgtcgt gacgctgata gctaaagctc agaatctggc gttcttcctg ccagttaagc
    28441 gcgagcgtga cgcggttttc cggcagcaac ggagactgga attttatgtt ctgaatggag
    28501 tgaaaacgcc agccaggcgc gagcagattg agcgcgtagc tcatcgccca gtcgatttgc
    28561 gcaactcctg ggagtaatgg ttgcacggca aaatgaccgc gaaaccagaa cagatccgcc
    28621 ctgagatgca gaacaatttc aaggtgattc gtttgtgcct gatggcgctc gatttcatgg
    28681 ggcttcatga aataactcct gtaattgcgc atagacacgc ttgttcatac tgtttactgg
    28741 catttcatca ataacgcgcc agtaacgcgg aatagcaact ggctccagcg tcgggcgcag
    28801 taaacgccgc caggtcattt cctggctatg cccgccgccg ttctgccatt gcagatgagc
    28861 ttcgtcatcc agcaccagca aaacgcctat gctctgacgc ccgccgcgcg taacgggaac
    28921 tgccgccgcc tcgcgaatgc cgtccagcgc cagtagacgt tgttctactt cctggagtga
    28981 aatacgtttt tcttcaatct taacgatgcg cccacgacgc cccatcagat ggaactgacc
    29041 atcttcgcta aactgtaaga tatcgtcgag taacatgccg ttatcttccg ctatcagcgg
    29101 tgaaaagagg cgaaaggcat cgccttcggc ctagaactgc atacctggga ggggtagcca
    29161 gcggcgttgc tcttgttcgc ggtagcgcca ggcgatgacg ccagtttccg tgctgccgta
    29221 gatttcatca ggccagacgc gcagccagct cgcggtatgt tgtacatcct gccacggcag
    29281 ctcaccgcca gcagagagaa gcacctgaac gggtggcgga gaaagctggg tatccaggcg
    29341 cttcaaaaat gccgggctgc tgatgaagat atagcgatgc tgatgactca acgcggcaaa
    29401 ctgttcgaca taccagagca tggcggcgtg cagcggtaat cccaacgcca tgggcaggaa
    29461 aacgcgaaaa gtcaggccgt acaaatgctg cggcaatacg gaaccgacga cacgacaacc
    29521 cgccagacgt gcgcctaatc gttcagccag cagttctgct tcacgatcca gcaaatgaac
    29581 aggtttaatc gcccgtttcg gctgtcccgt ggaaccggag gtaaacagct cgataaaagc
    29641 ctctggcgct atagcggcaa aggtgaagga ttgtgtcgcg atttgtggag aacttgctac
    29701 caacagcaat gaaccttgcc agttaagttc gctgtcgcta agcacaccat caaaaagttc
    29761 acgttgctcg ttaagctgga taacacggtt atgccccgga agcaccggcg tttttccggc
    29821 gtgtagcgtg gcgagcaggg caacgataaa cagatagctg ttctcaaagc acagcgccca
    29881 gcgttcaccc ggctgttgct gcaagcggca gataagttgc gcgacatcgt gacgcaaatc
    29941 gcccagcgtc caggtacttt cgttcagcca cgcaatgggt gtgtcatcag ggcgtggcgc
    30001 agtcagccag cgtgcaagcg gtaaggtctg cttcatgcgc gatctctttt catcacctga
    30061 cggcgcagca gccattcacc agccattaac gtccccatca acaaataagc aatcatgccg
    30121 ttccaggcgg tccataacga catatcggca taaagcgcgg taaagagggc aattccaccg
    30181 ttaatgatga aaaagccgca ccagatttgc gtgacgtggc gggtatagcg cacgcccttt
    30241 tccgggaggt ctggctcctg caaccgcgcc agccgctcaa ctatcggcat tgccgaccac
    30301 agcgaaccgc caaaaaccgc cagcatgacc gcgttaacca ctaccgggta aaacagcagt
    30361 agctggtgcg ttttcaacat aaagctggcg acacataatg cgatgcccac gacagcgacg
    30421 agttgcgtga cggcttgtaa aggccccgcc tggcgacggg tctggcgcag acgcaacaaa
    30481 agcagcaacg ccattagcgg taacagccag tgcaggccgt tatgcgctaa gccaaaccaa
    30541 atcacaaacg gccaggcgag cagcaataat ccagtcagca gcttaatcat cggtagcgag
    30601 cgaatgcccg acatcagatc aggcttcttg tagcaggcgt tctacagcct cgacgacatc
    30661 ctggactgta cgcactgctt taaactcttc cggcttgatt ttcttaccgg ttttcttttg
    30721 caggtgcaca atcatgtcaa cggcgtcgat gctgtccagt tccagatctt cgtacaggcg
    30781 cgtttcaggt ttgatgtcct gcggatcgat ttcaaacagc ttaaccagca gggctgagac
    30841 ttcctgatag acggtttgtt gttctgtcat gatggctttc ctcaggcacg ttgcgcagca
    30901 atgaaagagg ccagagtggc gacggagaaa aagtgctgac gcatctcttc gctttccgca
    30961 gagagcacga tgccgtactc attttttact gccagtccca gttccagggc atcgatagag
    31021 tccagcccta aaccgtcgcc aaacagcggc gcgtcggtat cgatatcatc aggcgtcaac
    31081 tcgtccagat tgagggtgct gatgatgagg tttttaattt ccagataaag cgcttgcatt
    31141 atttattcct gagagaggta aacaaccttg ttgtaattga agcaaaagat gccggtttaa
    31201 ctgccttgct gccagcgccg gttcttgtga agttgcatcg taaaatttat tgatttcaac
    31261 ccgaccatgg actgcaaccg taaaaaacgg ccggtcaggt ggaacgtcat accatttact
    31321 ttgtttatcc agcagatgct cgctacagcc gattgtcacg atgcgtaaat cgctggcgca
    31381 gcgcacggca atattcgccg ccccgcgctg cagcgtcatt ttttcaccgg gcttcgttcg
    31441 tgtgccttcg gggaaaatca ggatagtgtc accctgtgcc agtcgctgtt ggcagcgggg
    31501 taacagagtt tcagcttcgc tgttgattaa ataatctgtc gcccgcacga cgccgccaag
    31561 aaaaggattt tttaacagcg cgcttttcac caggcagtca gtttctggca tgactgacgc
    31621 cagcagcacg taatcgatca gcgtgggatg attggctacg acgaggcaac cgcgttcctg
    31681 atgtaagatt tcagcgccat caatacgata atccagtacg ccaagccctt tcgcgacagt
    31741 taaaaacagg cgaaaactgg cggcaatgct gcgacgcgcc aaacgacggc ggcgggaagt
    31801 atctcacacc aaaactaaca atatgttaaa ccacacgaca gaaagcagca ggccgccgag
    31861 accaaacagg gcaaaacata acccggtcat gaccagtcgc caggtccact ccagacgtga
    31921 catcagctta ctcatctgtg gctccagcgc cattgcacgc gctcgccagg cagtgaaaat
    31981 gtgtctacat tttgtaaata gtgctgtaaa aacaacatgc tttgcggcag tgttgtttca
    32041 ttgcccgcaa tggctgactg cgtttcacat tgccagtcat cgccagcttc aatcaccagc
    32101 gcgacggcgt acggccaggt cggcatttct gatggcagtt gtgggtgata aaactcgggc
    32161 aggaaaccgt caaaatcgac catcagcacg cgctggtatc ccgcttgtag caggctaagc
    32221 acttcacaca aaccttgctg gaaactatcg cgaccagccg aaagcgatga cgaaacgctc
    32281 ggctgtttgg ccgcaatggt caggttaccc acggaagagt tgtgtacgga gagcgcgaag
    32341 tccgtcggag aaagcgcctg ttcggttgcc agtgcatgaa caatgcggta attgcgctcc
    32401 agttcgccat gacgactggt atacaaaacg gcgtctggtt gatggcggcg tagcattgcc
    32461 agaccgcatt caacagccag tttgctcccg gagctgaggc ggcgggcggt catcattggc
    32521 agttcactta attttgccaa cggtgctgtc gggtcaattg cctgaggctg acgtgaccac
    32581 tcctgccatt gcagcgtttc actaagtcca ggcgctcttg cctgccagtc gagaatgttt
    32641 aatgcaaatt tcatcaacgc gcgcccgcgg taaaccaaaa ttattaagtg acgattattt
    32701 cttttggcat atcaaaagtg tatgcccaac gcctaaatta tcgtggcgct ctgctacctg
    32761 gaatcctgct ttattcagat agtcgtaaaa tttctctgca ctatagaaac ggctgttacc
    32821 gtttgccatg caggtaaaat aaagtgagga cgcgttcagg ctaaacgatg ctgcttcaaa
    32881 ccgctgcgca tcccagaaca actccatgat gcaaactttt gcgccaggct tcattacgct
    32941 ggcaattttg ctgagtatgc taattatttg ttcgggtgaa aaacagtcta aaaattggct
    33001 catccaccag atatcagcct cgcccggtaa tggggcatcg cttagcatat ccaccgcatg
    33061 gaaatctatg cgatcagaaa aacctgcatt cgcgatattt tctttcgcta gtacgatttg
    33121 ttgtggcaga tctaataacg taacggcgat gttttcattg tatttgcagc aacgcaaagc
    33181 ccacttaccc gtattgccgc ctacatcgta caatgttgtg gggttgttgg cgaatacata
    33241 aggcagtgcg gcgttaaatg cgccatctga atagtaatga tcaaacgcaa accagctttc
    33301 acgagcggcg tctggtaatt gcgaaagtgc cggatatatc gtcgaccagt cgccaaatac
    33361 ttttaacccg gaaggttttc cttcattgag tgaatcggcg aggaagaaga gcccctggta
    33421 acagacatcc tgggtgaaat ccatattcac gcgagtcata gtgtcatgca ggaggtaatg
    33481 accgattttc gccaggtaat aaattccctc tttacaggta ataattcgcc cgcttaagcc
    33541 catatctaac agaacgctgg tagcatattc gttgatatgg ctatgttcgg tgatatcagc
    33601 cagcgttgcg ccatgtttgc cttgccgatc cagatatgaa agtacacccg cattacgtag
    33661 gcagagtgct gtttgaaaaa gcataggggc aaaggcaatg cgttgagctt cggtaatcgc
    33721 atccagtgcg ctaagagaat cctgttcgta catgttgggc acaacctgtt aagagggaat
    33781 taaattatta tcggaccgca acggcccgac tttaggaaga attataaatc tgcgcccgct
    33841 gacaaattca gttgaatatc tttgtcgaga ttgcggaccc agcgattgta gtttttatga
    33901 atttgtccgc ctgacgcttg taggttctgg ctattttcat aattgatatc gtagctggat
    33961 gccgtgtagt taatgcgtat ttccgctgaa tgatcgcgtg actgttgacg tgctttaatt
    34021 atgccttgtc cggcttcgga cataatccat ttgcgttcaa cgccagcttt cagaatcgct
    34081 tttttcactt gttcctgggt atgaccagcg ctgacggttg agtgaacctg atcaattggc
    34141 gcagtacgcg cgcaacctgc taaagcaccc acaacagcaa tagccgcaaa ccatttcatg
    34201 taatgtctca ttagaacccc attaaagtat tgtttttacg agtaaagcaa gttttaactg
    34261 taattgctgg catcactatt ctttatttag agaaaaaatt acttgtgaga taagtcagta
    34321 agtgcacatc agagtaaaaa tctctgaaat gactaaatta ctgccagcag ggaccaacta
    34381 taagtgagtg agaaaaatga ggcaataaaa ataacacaat gttatcagtg cattctttga
    34441 gaaggattta atcgctattc ggcgtttata tatatttgac ttaaggaatg gtttttttaa
    34501 attaattttc tgaataatgt ttcatcattt cctcaaatca aatggccact gttagataaa
    34561 cggtggccat tgcctgctca ggaggtgcta ttaacccggt aaccgacttt tcggtctgcc
    34621 cggccccagt aaaatcgcca gtttgctgcc gcctttcgtc gtttccatcc agattttaca
    34681 cacgctggtt aatggcaccg aaagtagcat ccctaccggg ccgagcagcc atccccaaat
    34741 taacaatgaa agaaatacca ccatggtgga catccccagg cgatggccca tcatccgtgg
    34801 ttctaaaata ttgccgatga ccatatggac cactaaaaac aatgcgccga ccagaataca
    34861 ttcgtaaaca ccattaaaca gcagcacctg aatcattggc ggtacggcgg aaattaccgc
    34921 gccgatattg ggcacgtagt taagcaaaaa cgccagtact gcccacatca gcgcaaactg
    34981 tacccccatc agctccagcc ccagccagac gatgacaccc gtccataaac tgagtagcgt
    35041 ctttaacgcc agataatgcg aaacgccttt aagtgcgcgg tgtaatcccg cgatgtgaat
    35101 ctgtgggtta ttcagcgcaa aacgcatttt gtaagggacg tggcgcactt caaacagcat
    35161 aaaaactacg gtcatcacca gcaaaagcac gctcgccatt gccccggaaa gcccggtcat
    35221 tagcgctgtg gtgaaggtaa ccactttttc cgagtccatc cgctgcagca ttcgctccgg
    35281 cgacatatgc aaattaagaa aaggcaacat ctcctgcaat ttaaaaagtt tgcgcgtcag
    35341 ctccttatta aacttcggca gcatagagat aaattcgtta aacgatgccg ccagtacgcc
    35401 gaccagcgcg gttagtgcga tcagcatcac caccactaca atcgtaatgg caacggggcg
    35461 ttgtactccc cgacgaataa accaggtgac gagcgggttg aggacgatgg caaaaaacag
    35521 cgccagtaac agctgcacaa tgatatctgc cgctgcgtga atgcccgcga ggatcactac
    35581 cagcgaggcc agcttgagca gaatgtgcat gcccgtttta tcgggttgag gggtttccat
    35641 tggggcttcc ttgtgacttt ttgtattaag tgtagcggga gtcacgacgg caatattctg
    35701 attcttactg ctgattttca cgtcagcacg tggtaaaaat gaaacactgt tgtaaaaatg
    35761 tggtgatcct catgcccgaa cccgtagccg aacccgcgct aaacggattg cgcctgaatt
    35821 tgcgcattct ctccattgtc atgtttaact tcgccagcta cctcaccatc gggttgccgc
    35881 tcgctgtatt accgggctat gtccatgatg tgatgggctt tagcgctttc tgggcaggat
    35941 tggttatcag cctgcaatat ttcgccacct tgctgagccg tcctcatgcc ggacgttacg
    36001 ccgatttgct gggccccaaa aagattgtcg tcttcggttt atgcggctgc tttttgagcg
    36061 gtctgggata tctgacggca ggattaaccg ccagtctgcc cgtcatcagc ctgttattac
    36121 tttgcctggg acgcgtcatc cttgggattg ggcaaagttt tgccggaacg ggatcgaccc
    36181 tgtggggtgt tggcgtggtt ggctcgctgc atatcgggcg ggtgatttcg tggaacggca
    36241 ttgtcactta cggggcgatg gcgatgggtg cgccgttagg cgtcgtgttt tatcactggg
    36301 gcggcttgca ggcgttagcg ttaattatta tgggcgtggc gctggtggcc attttgttgg
    36361 cgatcccgcg tccgacggta aaagccagta aaggcaaacc gctgccgttt cgcgcggtgc
    36421 ttgggcgcgt ctggctgtac ggtatggcgc tggcactggc ttccgccgga tttggcgtca
    36481 tcgccacctt tatcacgctg ttttatgacg ttaaaggttg ggacggtgcg gctttcgcgc
    36541 tgacgctgtt tagctgtgcg tttgtcggta cgcgtttgtt attccctaac ggcattaacc
    36601 gtatcggcgg cttaaacgta gcgatgattt gctttagcgt tgagataatc ggcctgctac
    36661 tggttggcgt ggcgactatg ccgtggatgg cgaaaatcgg cgtcttactg gcgggggccg
    36721 ggttttcgct ggtgttcccg gcattgggtg tagtggcggt aaaagcggtt ccgcagcaaa
    36781 atcagggggc ggcgctggca acctacaccg tatttatgga tttatcgctt ggcgtgaccg
    36841 gaccactggc tgggctggtg atgagttggg cgggcgtacc ggtgatttat ctggcggcgg
    36901 cgggactggt cgcaatcgcg ttattactga cgtggcgatt aaaaaaacgg cctccggaac
    36961 acgtccctga ggccgcctca tcatcttaaa acttactgaa taaccagcgt attaatgatg
    37021 ttttctgcgg tggtctgcgc tttttgctga tcgtcagctg gcagagtaat ttgcatggtc
    37081 agcagttgat tacccacgtt acccagaata acggaagagt acgccgtctg gcctttcgcg
    37141 gagataatac tgtctaactg ctgcattttg tgacctttca gctcaatggc tttattggtg
    37201 accacttgca gctgtggatc gcggctacgt tgctgatctt ccagacgctt cgccagcacc
    37261 gccagatctt ctttcggatc atcgcccatg atgacgatga ctgctttctg cccggtggcg
    37321 tcggaccaga catgcatgtt attggcctgc gttcccagct taccgctctg gtcggtcata
    37381 tccgctggca gcgagaaact taacttgcca tcaagcaggt tgacgggatt cccggtagcg
    37441 ttactttccg cgaccgaacc ctgcgccgta gcgttagtgt ctttatcatc acaggccgca
    37501 agccccataa ccagcaggcc aattccgaca tatttaacca gattgcgcat tgacttcttc
    37561 ctttcgataa acggccataa cggctcattc atccatctta tcacaactct gataacgaac
    37621 ctttaactcg cctgcaaagc gttgatttcg gatttatctg ccagtctttt caacagcata
    37681 ttgagtaata cgccatacat tggcaggaag aaaacgatac tgattaacac tttgaaacag
    37741 taatcgacca gcgcgatttc catccagtgt tcagccataa aggcatccgg gctacgccag
    37801 aaggcaatga agaaaaaggc cagcgtgtcg ctgacgttac cgaacagtgt ggacgctgtc
    37861 ggtgccagcc accagcggcg actctgacgc aggcggttaa aaacgtgcac gtcgaggatc
    37921 tgtcccagcg cgtaggccat gaaactggcg gtggcgatac gggcgacaaa caggttgaag
    37981 tgggcgagtg cgccgaatcc ctgccatgaa cccatataga atagcgacga gatgacgtag
    38041 gagatcagca atgccgggat catcaccgca aagatgatac gacgggccag cggtgcgcca
    38101 aaaatacgta cggtcaggtc ggtagcgaga aagataaacg gaaagctaaa cgcgccccag
    38161 gtggtatgga aaccaaaaat ggagacggga agctgaacca gatagttact ggaggtgatc
    38221 accagcagat gaaataacga taaccagaac aacgccttat agcgttgagt ttgcgagaaa
    38281 acgttcatat tgtacctttt tgattaacca ttggggtgag ggaacccaat acgtacgaca
    38341 cgtctgttac cttaacataa taacgtgctt accctttttt cgagccgccg catgatactg
    38401 ctttacgttg acaatgcaat ggttaatttt cacgcaatcg ttaacctggt ttgcttacgg
    38461 actcacaggg cgtaaactag cgccgttttt ttatgtgatg agaagaaaat gaccgatctc
    38521 ttttccagcc ctgaccacac actcgacgca cttggcctgc gctgcccgga accagtcatg
    38581 atggtgcgca aaacagtgcg caatatgcag ccaggtgaaa cattgctgat tattgctgac
    38641 gatccggcga cgacccgcga tatccctggg ttttgtacct ttatggaaca cgaactggtt
    38701 gccaaagaga cggatggact gccttatcgt tatttgattc gtaaaggcgg ttgacggggg
    38761 cagattggct tcgatgccgc cttttcccct caccctaacc ctctccccag aggagcgagg
    38821 ggaccgatcg cgctcaatgt tgcgatcggt ttgccttatc tcctgcgcaa caatcttaac
    38881 gcattcgctg tcaccagcac cgtcgcaccc gtatctgcca gcactgccag ccatagtccg
    38941 gtcatcccta acagcgtggt gacgaggaag atccctttca gccccagcgc aatagtgatg
    39001 ttctggcgga tattggcgtg agtggcgcgt gccagttcaa tcatttgcac cagaccgcgc
    39061 aggtggttat gggttaatgc tgcgtcggcg gtttccagcg ccacgtctgt gccgctgccc
    39121 attgcaatcc cgatggcggc agctttcatc gctggcgcgt cgttaatacc gtcaccgacc
    39181 atcgccagcg gcgcatgttg attcagctcg gtcaccgctt tgactttatc ttccggcaac
    39241 aggcccgctt taaactccag ccccagctcc ccggcaattg ccgccgctgc gcgtggatta
    39301 tcgccggtga ggatcacccc tttgacgccc agcgcgttca gttcactgat ggcagttgca
    39361 gcatcggcgc gcagggtatc ctgcaatgca atgataccca gcacgtcatc attacgtact
    39421 accagcacaa ccgtttgccc ggcgctttcc agttcattaa tcaaaccagc aaatgcatca
    39481 gcgggatgtt tcccggcagc gcatatcaac acgcgctcac cgttaacctg cgcttcaatg
    39541 ccagacccga ccagcgcccg ctgtgattcg gcggcgggaa tggcgagtgc agcaacctgt
    39601 gcttcgcgca cgatggcctg cgccagtgga tgcgtcgcgc cttgctcgac cgccgccgcc
    39661 agtgtcagca gttcagattc actaataccc gttgccggat gaatcgcggt aacgcgcggt
    39721 ttaccgacgg tcagcgtacc ggttttatca aacgccacct gggtaacacg acccagctgt
    39781 tccagcgccg ctccgccttt aatcaacgcc ccacgacgcg ctgccgccgc cagcccggag
    39841 gtaatcgccg caggcgttga gataactaac gcacacgggc agccaatcag cagcagcgtc
    39901 agccctttat aaatccactc ctgccagctg gtggcaaaca gcagcggtgg caccagcgtc
    39961 accagcagag cgacggccat aatcgcgggc gtgtaaatac ggctgaaacg gtcgataaac
    40021 cgctcaatgg gtgcgcgacg ctcttcggct tcttcgatca gtttcagaat ccggtcaatg
    40081 gcgctggcac ccggttctga cagcacttcc agcgttacca gacggtctac gctggtggct
    40141 cctgcaggaa ctttatcgcc cgttgcgcgc tccaccggaa tagattcgcc ggtcagggcg
    40201 ctttcatcaa aactggcaaa cggtgagagc agtttaccgt cggcaggcaa acgtccacct
    40261 gcggcgactt caatcacatc gccagggcgc aggctgttaa tcgccacctc ttcccgctca
    40321 ccgttacgca ggcgcgtggc ggtttctggt ttcagcgcca ttaacgcgct gaccccttga
    40381 cgcgcgcggc tggcggccca gccttccagt cgttcaccaa tcaaaaacag cagcaacacc
    40441 atcgcagctt cagccgttgc gccaataaac agtgcaccaa tagcggctac gctcattaag
    40501 gtttcaatgg cgaagtagct gccggatttg atcagccgta atgcctgacg agcaatcggg
    40561 tacagcccaa ccagcgtggt cgcgataaac gccagttgcc cgaacggatg attaaattgc
    40621 tccaaacccc agctgattgc catcatcacg ataagcgtaa tcagcggcag attctctttc
    40681 aggcgcgatg cttgtggctc gtcggcggcc tgctcatcgc gcagggaata gcccgctttt
    40741 tgcaccgcag attcaacttg tgcacggatg tcattgtcgg catcaaccac cagtttttcg
    40801 gtggcgaaca acacctgtac ctgattcacg cctgcaagct ggcgcacggc attttctacc
    40861 ttgcgcgcac aggcggcgca gtccatgccg ctgactttcc agctatagcg ggtgccggag
    40921 acgttttcag agagagttgg cgtgctggaa catgcgccgt cgcagcaaca gtcgttggcg
    40981 ttctgtaccg tggttagcgg tttgaacgca gcaaattgag gggctttctt gccgtgattg
    41041 tcaggagtcg acatggcatc ctccggttaa gttttttctc attaaccgaa ggatacactc
    41101 tggagtcgac tccagagtca agttttatca gagatacagc gagcggacga tgaggaagtg
    41161 accggcaaag tagcaggccg cagcaatcgc gttgtccgca cggaagcgtc ggcgatagtg
    41221 actacccagc cagacaaagt tactgataaa cagcaacgaa gcgccgacaa acgcagagag
    41281 tgccggagcg gtcggacgga agaaccacag ttcacctgcc agccacacca tcaccagcgt
    41341 catgccgata aacgtgcaga taggccaacg atactcttcc agccgcgtcc agataatcgc
    41401 cagtaacagc gcacccagaa ccagcaacac cagtggcaga ggccagaaga aagagagcgt
    41461 catctgactg gcgaaataga tggtgtacag caggtgcgag aggaaaaacg cgccgatggc
    41521 gtacatcaga cgttgacgtg gcaacagggt tagcgcatcg cccagcagtg aggcgcacag
    41581 ccctgccagc accagatagc taatggcgtc gaacatcggc gcttgccagg ccagtaacag
    41641 caggagaaga agggttaacg gtttaaacac ccagcgttgc caggttggcc cacgatacga
    41701 tgcatccaca gatagccatg cggaaagaca gacagcgata aacgaccaaa gcatcttagt
    41761 tccttgaatt cgttattttt tcgtaagcgg aatgcctacg gtcttgcttc cagtttagtg
    41821 gcgagggtgg acggatgaca acaccataac tggcagggta tgcttatttc cgcattttcc
    41881 gatgagggat aaagatgagc aaaccacctc ttttctttat tgttatcatt ggcttaatca
    41941 tcgtcgccgc atcgtttcgt tttatgcaac agcgacggga aaaagctgat aatgatatgg
    42001 caccactcca gcaaaagctg gtgttggtga gcaacaagcg ggaaaaaccg attaacgatc
    42061 gccgttcgcg ccagcaggaa gtgactccgg caggcaccag tatgcgctat gaggcaagct
    42121 tcaaaccgca aagcggagga atggagcaga cgtttcgcct tgacgcccag cagtaccacg
    42181 ccctgacagt gggcgataaa ggtacgctga gctataaagg aacgcgcttt gttagctttg
    42241 ttggcgaaca ataaggttat tttttaactt taaatttctt ctgccagacc agcagttcaa
    42301 acacgccgaa aaggaaaatc cgcacctttt cggcggtggt catctgcggg ccatctttcg
    42361 gtaaagtgga tttcaatagc gccagttgca taccatgcat cagcacggta aaaatcagcg
    42421 ccacgttaac gaaaatattc agcgggcgcg ggaagggatg caccaggttg aggattaaaa
    42481 atccccaaac acagagcatt aacaaacgac caatattaat cagcatcact ttctccttgt
    42541 gcttcgcgtt gatacagccg ataagccacc tgacccgcca ctttttcccg atgtaacgac
    42601 cagtttgccg gaacggtggg cagaccgttt tcgacttcgc tttcgacata aatcagggtt
    42661 tcgtcagcca gccagccgtt atcttccagt aaatttatcg tctcttctaa caaaccacgg
    42721 cggaacggtg gatcgacaaa cacgatatta tgcggtgtgc ctttttgcgc caggaatgac
    42781 attgcgttgc tgttcaccac gcgtgcattg cctgctttta gtgtcgccag attcttaatt
    42841 aactgctgag aaactgcacg atccatctca attaacgtgg cccccgcagc gtagcgcgat
    42901 aacgcttcca gccccagcgc gccgctcccg gcgaagcaat ccagacattg ggcgtcaaca
    42961 atgaccggag ccagccagtt aaacaacgtt tcgcgtacgc ggtcggtggt ggggcgcaga
    43021 cctgggctat caggaaccgg gagtttacgg cctcgccact gcccgccaat aatgcggatt
    43081 tggccgctgc cggaatgatt cggttttttc atgataaatt gctcaatccg ccagataaca
    43141 catacttgca ggcggtgatg tgaattaagt taagtgatag actatttcat cattttttta
    43201 gctgctatgt acatagcgtt aacgctgtgc catgaagcaa cagcgaggag tgtagtcgca
    43261 aatggcgaaa gaaaaaaaac gtggcttttt ttcctggctg ggctttggtc aaaaagagca
    43321 gaccccggaa aaagagacag aagttcagaa tgaacaaacg gttgtagaag aaatcgttca
    43381 ggcgcaagag cctgtgaagg cttctgaaca agccgtcgaa gagcagccgc aggcgcatac
    43441 tgaggccgag gcggaaactt ttgctgccga cgttgtggaa gtcactgaac aggttgctga
    43501 aaatgaaaaa gcgcagcctg aagcggaagt cgttgcacaa ccggaaccgg tcgtagaaga
    43561 aacgccggag ccagtggcta tcgaacgtga agagctgccg ttgccggaag acgtcaacgc
    43621 cgaagaggtt tcgccagaag agtggcaggc tgaagcggaa accgtagaga ttgtcgaagc
    43681 ggcggaagaa gaagcggcta aagaagaaat caccgacgaa gagctggaag cacaggcgct
    43741 ggctgccgaa gcagcagaag aggcggtgat ggtggttcct ccggcagaag aagagcagcc
    43801 ggtggaagaa atcgctcagg agcaggaaaa accgaccaaa gaaggttttt tcgcgcgcct
    43861 gaaacgcagc ctgttaaaaa ccaaagaaaa tctcggttcc ggatttatca gcctgttccg
    43921 cggtaaaaaa atcgacgatg atctgtttga ggagctggaa gagcagcttt tgatcgccga
    43981 tgtgggcgtg gaaaccacac gtaaaattat caccaatctg acggaaggcg catcccgcaa
    44041 gcagcttcgt gatgccgagg cgctctatgg cctgctgaaa gaagagatgg gtgagattct
    44101 ggcgaaagtc gatgagccgc tgaatgttga aggtaaaacg ccgttcgtga tcctgatggt
    44161 gggcgtcaac ggtgtgggta aaaccacgac gattggtaag ctggcacgtc agtttgagca
    44221 gcagggtaaa tcggtgatgc tggcggcggg cgatactttc cgtgcagcag cggttgaaca
    44281 gcttcaggtc tgggggcagc gcaacaatat tccggtgatt gcccagcata ccggggcgga
    44341 ttccgcctct gttatcttcg acgccattca ggcggctaaa gcgcgtaata tcgacgtcct
    44401 gattgccgat accgccggac gcctgcagaa caaatcgcac ctgatggaag agttgaagaa
    44461 aatcgtccgc gtgatgaaga aactcgacgt tgaagcgccg catgaagtta tgctgactat
    44521 cgatgccagc accgggcaga acgcggtaag ccaggccaaa ctgttccatg aagccgttgg
    44581 cttaaccggc atcacgctaa cgaaactgga cggcacggcg aaaggcgggg taattttctc
    44641 ggtggctgac cagtttggta tccctatccg ctacattggt gtcggcgaac gtattgagga
    44701 tttgcgtccg tttaaggcgg acgactttat agaggcactt tttgcccgag aggattaaca
    44761 atgattcgct ttgaacatgt cagcaaggct tatctcggtg ggagacaggc gctgcagggc
    44821 gttacgttcc atatgcagcc gggtgagatg gcgtttctga ccggtcattc cggcgcaggg
    44881 aaaagtaccc tcctgaagct gatctgtggg attgagcggc ccagcgccgg gaaaatctgg
    44941 tttagcggcc atgacatcac gcgtctgaaa aaccgtgaag ttccgtttct gcgccgccag
    45001 attggcatga ttttccagga tcaccatcta ctgatggacc gtactgtcta cgataacgtg
    45061 gcgatcccgc tgattatcgc cggtgccagc ggtgacgata ttcgccgccg ggtgtcggcg
    45121 gcactggata aagtcgggct actggacaaa gcgaagaact tccctattca gctttctggc
    45181 ggtgaacaac agcgtgttgg cattgcccgc gcggtggtga acaagcccgc ggtactgctg
    45241 gcggacgaac cgactggtaa cctggacgac gcgttgtcgg aagggattct acgtctgttt
    45301 gaagagttta accgcgtagg ggtaaccgta ttgatggcaa cgcacgacat caacctgatt
    45361 tcacggcgtt cctatcgcat gctcaccctg agcgatggtc acttgcatgg aggcgtgggc
    45421 catgaataag cgcgatgcaa tcaatcatat tcggcagttt ggcgggcgtc ttgatcgctt
    45481 ccgtaaatcg gtcggcggct caggcgacgg cggtcgtaac gcaccgaaac gcgcgaaatc
    45541 ctcgccaaaa ccggtaaatc gcaaaaccaa cgttttcaac gaacaggtgc gctatgcctt
    45601 ccacggcgca ttgcaggatc tgaaaagcaa accgttcgcc acatttttaa cggtgatggt
    45661 tatcgccatc tccctgacgc tgcccagcgt ctgttatatg gtgtacaaaa acgttaacca
    45721 ggcggcgacg cagtattatc cgtcaccgca aatcactgtt tatctgcaaa aaacgctgga
    45781 cgatgacgcc gctgcgggcg tggtggcaca gttgcaggcc gagcaaggcg tagagaaagt
    45841 gaactatctt tctcgtgaag acgcactggg cgagttccgt aactggtctg gttttggtgg
    45901 cgcgctggat atgctggaag aaaacccgct tccggcagtg gcggtggtga tcccgaagct
    45961 cgactttcag gggacggagt ctctgaatac gctgcgtgat cgtatcacgc agattaacgg
    46021 cattgacgaa gtgcggatgg atgacagctg gtttgcccgt ctggcggcgt tgaccgggct
    46081 ggtcgggcgc gtttcggcga tgatcggcgt gttgatggtg gcggcagtgt tcctcgtcat
    46141 cggtaacagt gtacgcctga gtatctttgc tcgtcgtgac tccattaacg tgcagaaact
    46201 gattggtgca acggatggtt tcatcctgcg cccgttcctg tacggtggcg cattgctggg
    46261 gttttctggc gcattgttgt cattaatttt atcagaaatt ctggtactgc gattgtcatc
    46321 ggcggttgcg gaagtggcac aggttttcgg aacgaagttt gatatcaatg gtttatcgtt
    46381 cgatgaatgc ctgctgttgc tgctggtatg ctcgatgatt ggctgggtgg cagcgtggct
    46441 tgccacggta caacatttac gccactttac gcctgaataa taaaagcgtg ttatactctt
    46501 tccctgcaat gggttccgta gcagggaaag agaccccgtt gtctcttccc ggtatttcat
    46561 ctctatgtca cattttgtgc gtaatttatt cacaagcctg cattgaactt gtggataaaa
    46621 tcacggtctg ataaaacagt gaatgataac ctcgttgctc ttaagctctg gcacagttgt
    46681 tgctaccact gaagcgccag aagatatcga ttgagaggat ttgaatgact gacaaaatgc
    46741 aaagtttagc tttagcccca gttggcaacc tggattccta catccgggca gctaacgcgt
    46801 ggccgatgtt gtcggctgac gaggagcggg cgctggctga aaagctgcat taccatggcg
    46861 atctggaagc agctaaaacg ctgatcctgt ctcacctgcg gtttgttgtt catattgctc
    46921 gtaattatgc gggctatggc ctgccacagg cggatttgat tcaggaaggt aacatcggcc
    46981 tgatgaaagc agtgcgccgt tttaacccgg aagtgggtgt gcgcctggtc tccttcgccg
    47041 tccactggat caaagcagag atccacgaat acgttctgcg taactggcgt atcgtcaaag
    47101 ttgcgaccac caaagcgcag cgcaaactgt tcttcaacct gcgtaaaacc aagcagcgtc
    47161 tgggctggtt taaccaggat gaagtcgaaa tggtggcccg tgaactgggc gtaaccagca
    47221 aagacgtacg tgagatggaa tcacgtatgg cggcacagga catgaccttt gacctgtctt
    47281 ccgacgacga ttccgacagc caaccgatgg caccggtgct ctatctgcag gataaatcat
    47341 ctaactttgc cgacggcatt gaagatgata actgggaaga gcaggcagca aaccgtctga
    47401 ccgacgcgat gcagggtctg gacgaacgca gccaggacat catccgtgcg cgctggctgg
    47461 acgaagacaa caagtccacg ttgcaggaac tggctgaccg ttacggtgtt tccgctgagc
    47521 gtgtgcgcca gctggaaaag aacgcgatga aaaaactgcg cgctgctata gaagcgtaat
    47581 ttccgctatt aagcagagaa ccctggatgc gagtccgggg tttttgtttt ttgggcctct
    47641 acaataatca atttcccctc cggcaaaacg ccaatcccca cgcagattgt taataaactg
    47701 tcaaaatagc tattccaata tcataaaaat cgggtatgtt ttagcagagt atgctgctaa
    47761 agcacgggta gtcatgcata aaacgaaata aagtgctgaa caacaacatc acaacacacg
    47821 taataaccag aagaatgggg attctcagga tgaacataaa gggtaaagcg ttactggcag
    47881 gatgtatcgc gctggcattc agcaatatgg ctctggcaga agatattaaa gtcgcggtcg
    47941 tgggcgcaat gtccggtccg gttgcgcagt acggtgacca ggagtttacc ggcgcagagc
    48001 aggcggttgc ggatatcaac gctaaaggcg gcattaaagg caacaaactg caaatcgtaa
    48061 aatatgacga tgcctgtgac ccgaaacagg cggttgcggt ggcgaacaaa gtcgttaacg
    48121 acggcattaa atacgtgatt ggtcacctct gttcttcatc aacgcagcct gcgtctgaca
    48181 tctacgaaga cgaaggcatt ttaatgatca ccccagcggc aaccgcgccg gagctgaccg
    48241 cccgtggcta tcagctgatc ctgcgcacca ccggcctgga ctccgaccag gggccgacgg
    48301 cggcgaaata tattcttgag aaagtgaaac cgcagcgtat tgctatcgtt cacgacaaac
    48361 agcaatacgg cgaaggtctg gcacgagcgg tgcaggacgg cctgaagaaa ggcaatgcta
    48421 acgtggtgtt ctttgatggt atcaccgccg gggaaaaaga tttctcaacg ctggtggcgc
    48481 gtctgaaaaa agagaatatc gacttcgttt actacggcgg ttatcacccg gaaatggggc
    48541 aaatcctgcg tcaggcacgc gcggcagggt tgaaaactca gtttatgggg ccggaaggcg
    48601 tggctaacgt ctcgctgtct aacattgcgg gcgaatcagc ggaagggctg ctggtgacca
    48661 agccgaagaa ctacgatcag gttccggcga acaaacccat tgttgatgcg atcaaagcga
    48721 aaaaacagga cccaagcggc gcattcgttt ggaccaccta cgccgcgctg caatctttgc
    48781 aggcgggcct gaatcagtct gacgatccgg ctgaaatcgc caaatacctg aaagcgaact
    48841 ccgtggatac cgtaatggga ccgctgacct gggatgagaa aggcgatctg aaaggctttg
    48901 agttcggtgt gtttgactgg cacgccaacg gcacggcaac cgatgcgaag taacagatag
    48961 ataaatgaaa atgcccgatg cgctgaactg ccatcgggtt tttttgtgtt tgtcgaaatg
    49021 gttgtagaaa gattaaacaa tccaggatat gcttaatgta cggtgacata ccgtacatat
    49081 tatacggagc aggagagttc acatgtctgc tgttaaaaag cagcgtatcg atctgcgttt
    49141 aactgacgat gacaaaagta tgattgagga agcggcagcg atatctaatc agtccgtcag
    49201 ccaatttatg ttgaacagcg cttcgcagcg ggctgcggaa gtgattgaac agcatcggcg
    49261 ggtgatcctc aatgaggaat cctggacgcg ggtgatggat gcgctgagta atccaccgtc
    49321 accaggtgaa aagctaaaac gtgcggcaaa acgtcttcag ggaatgtaat aatttatgga
    49381 tgatctgacg atagagattc tgaccgatga tgcagattat gatctacagc gattcgactg
    49441 cggcgaggaa gcgttaaatc tctttctgac gacacatctc gttcgtcaac atcgcaacaa
    49501 aattctgcga gcgtatatcc tttgtcgcaa cactccagaa cgtcaggtgc tgggatatta
    49561 cacattatgc ggcagttgtt ttgaacgagc cgcattgccc tcgaaatcga aacagaaaaa
    49621 aattccctac aaaaatattc ccagcgttac tcttgggcgt ctggcaattg atcgttcatt
    49681 acaggggcag ggatggggag caacactggt tgctcatgcc atgaaagtcg tctggtcagc
    49741 ctctttagcg gtaggtattc acggtctttt tgtcgaggcg ctgaatgaaa aagcccatac
    49801 gttttataaa tcgctgggct ttatcccttt agtcggggaa aacgaaaatg cgttattttt
    49861 cccaaccaaa tccattgaac tgctttttac acagagcgat taacgcttct cccagccgcc
    49921 ctgctgtgcc gtaaatccca gtgcctgcat aaacgccgtc atcacaccgc gatcttccac
    49981 gcctgcatcc gccatccacc agcatgaaac gccaggattg ttacgcaaaa cctcttccag
    50041 cagatattgc cccacaccgc gacgtcgagt gacttcccgc acgcgcaggg aatccagtgc
    50101 cccctcggtg ccgcttaagg ttacccgcac ggcagcaagc aggcgctcgt taaaacgcgc
    50161 ggcgtagatg cggtggttat cgtcaacctg taaagaggaa ggggaatact ccggccagat
    50221 cttttgcagg tcaatccggt cctggtcgct aaatttttct aatcgaatga tggtcagctt
    50281 catgggtaac ccgtgtaaat cacaaaagtg gaaccagtgt agcgaaataa tttaatcgga
    50341 ggctttctct tttttatttc ttttggcagg tgattaattt cttaacagca ataattacaa
    50401 agttaaaaca ttagagaatg aaaaataccc agcataatcc cctgaatggt agtgaattat
    50461 tccgcccttt gtgccgttat tttatgctga caaaggcact tttttctgtt tatctatcaa
    50521 taaattcaga atattatctg ttcttaatcg actgaaaaat agggacttta atcgctatta
    50581 tcacaaaata ctgcgctaaa cccttaatca gacaggcaaa aacagtgcag tataaaaaaa
    50641 gaacagtctg atttgttaac acataaaaac aaagcaacac aacatcacga atggggattt
    50701 ttgactatga aacggaatgc gaaaactatc atcgcaggga tgattgcact ggcaatttca
    50761 cacaccgctt tagccgacga tattaaagtt gccgttgtcg gcgcgatgtc cggcccgatt
    50821 gcccagtggg gcgatatgga atttaacggc gcgcgtcagg cgattaaaga cattaatgcc
    50881 aaagggggaa ttaaaggcga taagctggtt ggcgtagaat atgacgacgc ctgcgacccg
    50941 aaacaagccg ttgcggtcgc caacaaaatc gttaatgacg gcattaaata cgttattggt
    51001 catctgtgtt cttcttctac ccagcctgcg tcagatatct atgaagacga aggtattctt
    51061 atgatctcgc cgggagcgac caacccggag ctgacccaac gcggttatca acacattatg
    51121 cgtactgccg ggctggactc ttcccagggg ccaacggcgg caaaatacat tcttgagacg
    51181 gtgaagcccc agcgcatcgc catcattcac gacaaacaac agtatggcga agggctggcg
    51241 cgttcggtgc aggacgggct gaaagcggct aacgccaacg tcgtcttctt cgacggtatt
    51301 accgccgggg agaaagattt ctccgcgctg atcgcccgcc tgaaaaaaga aaacatcgac
    51361 ttcgtttact acggcggtta ctacccggaa atggggcaga tgctgcgcca ggcccgttcc
    51421 gttggcctga aaacccagtt tatggggccg gaaggtgtgg gtaatgcgtc gttgtcgaac
    51481 attgccggtg atgccgccga aggcatgttg gtcactatgc caaaacgcta tgaccaggat
    51541 ccggcaaacc agggcatcgt tgatgcgctg aaagcagaca agaaagatcc gtccgggcct
    51601 tatgtctgga tcacctacgc ggcggtgcaa tctctggcaa ctgcccttga acgtaccggc
    51661 agcgatgagc cgctggcgct ggtgaaagat ttaaaagcta acggtgcaaa caccgtgatt
    51721 gggccgctga actgggatga aaaaggcgat cttaagggat ttgattttgg tgtcttccag
    51781 tggcacgccg acggttcatc cacggcagcc aagtgaaaat cccaccgccc gtgaaatgcg
    51841 ggcgggttta gaaaggttac cttatgtctg agcagttttt gtatttcttg cagcagatgt
    51901 ttaacggcgt cacgctgggc agtacttacg cgctgattgc catcggctac accatggttt
    51961 acggcattat cggcatgatc aacttcgccc acggcgaggt ttacatgatc ggcagctacg
    52021 tctcatttat gatcatcgcc gcactgatga tgatgggcat tgataccggc tggctgctgg
    52081 tagcggcggg attcgtcggc gcaatcgtca ttgccagcgc ctacggctgg agtatcgaac
    52141 gggtggctta ccgcccggtg cgtaactcta agcgcctgat tgcactcatc tcggcaatcg
    52201 gtatgtccat cttcctgcaa aactacgtca gcctgactga aggttcgcgc gacgtggcgc
    52261 tgcccagctt gtttaatggt cagtgggtag tgggtcatag cgaaaacttc tctgcctcta
    52321 ttaccaccat gcaggcggtg atctggattg tcaccttcct cgccatgctg gcgctgacga
    52381 ttttcattcg ttattcccgt atggggcgcg cctgccgtgc ctgcgccgaa gatctgaaaa
    52441 tggcgagcct gcttggcatt aacaccgacc gggtgattgc tttgaccttt gtgattggcg
    52501 cggcgatggc ggcggtggcg ggcgtactgc tcggtcagtt ctacggcgtc attaacccct
    52561 atatcggctt tatggccggg atgaaagcct ttaccgctgc ggtgctcggt gggattggca
    52621 gcattccggg agcgatgatt ggcgggctga ttctggggat tgcggaggcg ctctcttctg
    52681 cctacctgag tacggaatat aaagatgtgg tctcattcgc cctgctgatt ctggtgctgc
    52741 tggtgatgcc gaccggtatt ctgggtcgcc cggaggtaga gaaagtatga aaccgatgca
    52801 tattgcaatg gcgttgctct ctgccgcgat gttttttgtc ctggcgggcg tctttatggg
    52861 cgtacaactg gagctggatg gcaccaaact ggtggtcgac acggcttcag acatccgttg
    52921 gcagtgggtg tttatcggca cggcggtggt ctttttcttc cagcttttgc gaccggcttt
    52981 ccagaaaggg ttgaaaagcg tttccggacc gaagtttatt ctgcccgcca ttgatggctc
    53041 cacggtgaag caaaagctgt ttctcgtggc gctgctggtg cttgcggtgg cgtggccgtt
    53101 tatggtctca cgcgggacgg tggatatcgc caccctgacc atgatctaca ttatcctcgg
    53161 actcgggctg aacgtggtgg tagggctttc tggtctgctg gtgctggggt acggcggctt
    53221 ttacgccatc ggcgcttata cctttgcgct gctcaatcac tattacggct tgggcttctg
    53281 gacctgtctg ccgatagccg ggttgatggc ggcggcggcg ggcttcctgc tcggcttccc
    53341 ggtgcttcgc ttgcgcggtg actatctggc gatcgttacc ctcggtttcg gcgaaattgt
    53401 gcgcatattg ctactcaata acaccgaaat taccggcggt ccgaacggta tcagtcagat
    53461 cccgaaaccg acttttttcg gccttgagtt cagccgtacc gctcgtgaag gcggctggga
    53521 cacgttcagt aatttctttg gcctgaaata cgatccctcc gatcgtgtca ttttcctcta
    53581 cctggtggcg ttgctgctgg tggtgctaag cctgtttgtc attaaccgcc tgctgcggat
    53641 gccgctgggt cgcgcctggg aagcgttgcg tgaagatgaa atcgcctgcc gttcgctggg
    53701 cttaagcccg cgtcgtatca agctgactgc ctttaccatc agcgccgcgt ttgccggttt
    53761 tgccggaacg ctgtttgctg cgcgtcaggg ctttgtcagc ccggaatcct tcacctttgc
    53821 cgaatcggcg tttgtgctgg cgatagtggt gctcggcggt atgggctcgc aatttgcggt
    53881 aattctggcg gcaattttgc tggtggtgtc gcgcgagttg atgcgtgatt tcaacgaata
    53941 cagcatgtta atgctcggtg gtttgatggt gctgatgatg atctggcgtc cgcagggctt
    54001 gctgcccatg acgcgcccgc aactgaagct gaaaaacggc gcagcgaaag gagagcaggc
    54061 atgagtcagc cattattatc tgttaacggc ctgatgatgc gcttcggcgg cctgctggct
    54121 gtgaataacg tcaatcttga attgtacccg caggagatcg tctcgttaat cggtcctaac
    54181 ggtgccggaa aaaccacggt ctttaactgc ctgaccggat tctacaaacc caccggcggc
    54241 actattttac tgcgcgatca gcatctggag ggtttgccgg ggcagcaaat cgcccgcatg
    54301 ggcgtggtgc gtaccttcca gcatgtgcgt ctgttccgcg aaatgacggt aattgaaaac
    54361 ctgctggtgg cgcagcatca gcaactgaaa accgggctgt tctctggcct gttgaaaacg
    54421 ccttctttcc gtcgcgccca aagtgaagcg ctcgaccgcg ccgccacctg gcttgagcgc
    54481 attggtttgc tggaacacgc caaccgtcag gcgagtaacc tggcctacgg tgaccagcgc
    54541 cgtctggaga ttgcccgctg catggtgacg cagccggaga ttttaatgct cgacgaacct
    54601 gcggcaggtc ttaacccgaa agaaacgaaa gagctggatg agctgattgc cgaactgcgc
    54661 aatcatcaca acaccactat cttgttgatt gaacacgata tgaagctggt gatgggaatt
    54721 tcggaccgaa tttacgtggt caatcagggg acgccgctgg caaacggtac gccggagcaa
    54781 atccgtaata acccggacgt gatccgtgcc tatttaggtg aggcataaga tggaaaaagt
    54841 catgttgtcc tttgacaaag tcagcgccca ctacggcaaa atccaggcgc tgcatgaggt
    54901 gagcctgcat atcaatcagg gcgagattgt cacgctgatt ggcgcgaacg gggcggggaa
    54961 aaccaccttg ctcggcacgt tatgcggcga tccgcgcgcc accagcgggc gaattgtgtt
    55021 tgatgataaa gacattaccg actggcagac ggcgaaaatc atgcgcgaag cggtagcgat
    55081 tgtcccggaa gggcgtcgcg tcttctcgcg gatgacggtg gaagagaacc tggcgatggg
    55141 cggctttttt gccgaacgtg atcagtttca ggagcgcata aagtgggtgt atgagctgtt
    55201 tccacgtctt catgagcgtc gtgttcagcg ggcaggcacc atgtccggcg gtgaacagca
    55261 gatgctggcg attggtcgcg cgctgatgag caatccgcgt ttgttgttgc ttgatgagcc
    55321 atcgctcggt cttgcgccga ttatcatcca gcaaattttc gacaccatcg agcagctgcg
    55381 cgagcagggg atgactatct tcctcgtcga gcagaacgcc aaccaggcgc tgaagctcgc
    55441 tgatcgcggc tacgtgctgg aaaacggcca tgttgtgctc tccgatactg gtgatgcgct
    55501 gctggcgaac gaagcagtga gaagtgcgta tttaggcggg taaattatcg ataacccggc
    55561 agctaagttc tgccgggtta ttcgtccgta tacagacttg tttcaactga ccgggaggat
    55621 taaaaccaga ccacttattt gccacggaaa ggttccctcc catgcattat gccgttgttg
    55681 tcatgcttga actgctttgc tgtattcacg cttaccgtac cggacaagag cggtactgga
    55741 tttttatcat tttctgtttt ccggtgattg gctgcgtggc gtatttcgtc atagtgatgc
    55801 tgcctgaaac gggcgctgac cgtcacgggc atactttgct gatgcgcctg caggacaaac
    55861 tcaaccctga gcgccacctg cgcaaattaa ccgaagaact ggcgattgcc gagacgaatc
    55921 aaaaccatta tgcgttggct aatgaactgg cgcgtctggg gcgttatcac gaagctgttc
    55981 cccattatca gcaggcgtta agcgggatat tcgcccatga agcggcaatg atgctaagcc
    56041 tggcgcaggc acagtttgct attcaggagt tcgccgcctg tcagcagacg ctggaagatg
    56101 tgatgcgtta taaccctgat tttcagtcgg cagacgggca tttgttattt gccagaacgt
    56161 tagccgcaca agaaaaatac gctgatgcgg aaagcgagtt tgaagtattg atttcttatt
    56221 accccggccc gcaggcgcgt atttactatg cggaaatgtt ggcgaaaatg agtcggttac
    56281 gtgaagccaa cgagcagtat gttgccgtgg ttgatacggc taaacgctca agacctcatt
    56341 atagaaaaca ccaccgggaa tggattaaga ccgccaatga gcggctgaag cagagtgtgg
    56401 tgcaatgaaa atcttctttg ccgttagacc agtgtcgcgg caatcttttg ttaaaattga
    56461 aaaatgtagc tctcatcggg cagcaaaatt ggacgcagaa tttgctgcca tttttgctct
    56521 tcatggtaag gagttctggc gattagctgg ccgttaatcg cctcttttca gtgcattctc
    56581 gggatatccg tgccccatct cccattttcc ctcacccgta ccgtcaccgc cttgtcatct
    56641 ttctgacacc ttactatctt acaaatgtaa caaaaaagtt atttttctgt aattcgagca
    56701 tgtcatgtta ccccgcgagc ataaaacgcg tgaattcgcg cattcggtac aacaagagag
    56761 ataaacgatg aaaccgttac attatacagc ttcagcactg gcgctcggac tggcgttaat
    56821 ggggaatgca caggcagtga cgaccattcc gttctggcat tctatggaag gggaactggg
    56881 taaagaggtg gattctctgg cccaacgttt taacgccgaa aacccggatt acaaaattgt
    56941 accgacctat aaaggcaatt acgaacagaa tttaagcgcg gggattgccg catttcgtac
    57001 cggcaatgct ccggctattt tgcaggttta tgaagttggc accgccacca tgatggcgtc
    57061 gaaagccatt aaaccggttt atgacgtgtt taaagaggcg gggattcaat tcgatgagtc
    57121 gcagtttgtg ccgacggttt ccggttacta ctccgacagc aaaacgggcc acttactctc
    57181 ccagccattc aacagctcga ctcccgttct ctattacaac aaagacgcct tcaagaaagc
    57241 ggggttagac ccggaacaac cgccgaaaac ctggcaggat ctggcggact atgccgcaaa
    57301 actaaaagcc tccggcatga agtgcggcta cgccagcggc tggcagggct ggatccaact
    57361 ggaaaacttt agcgcctgga acggtctgcc gtttgccagc aaaaacaacg gctttgacgg
    57421 cacggatgcg gtgctggagt tcaacaagcc ggagcaggtg aaacacatcg ccatgctcga
    57481 agagatgaac aagaagggcg atttcagcta cgtcgggcgt aaggatgaat ccaccgagaa
    57541 gttctataac ggtgattgcg ctatgaccac cgcctcttcc ggttctctcg ctaatattcg
    57601 cgagtacgcc aaattcaact atggcgtagg catgatgcct tacgacgccg atgcgaaaga
    57661 tgcaccgcaa aacgccatta tcggcggggc cagcctgtgg gtgatgcagg gcaaagataa
    57721 agaaacgtat accggcgtgg cgaagttcct cgacttcctc gcgaagccag aaaacgctgc
    57781 cgagtggcat cagaaaaccg gttatctgcc aatcaccaaa gcagcgtatg acctgacccg
    57841 tgagcagggc ttttatgaga aaaacccagg ggcggatacc gcgacgcgtc agatgctgaa
    57901 taagccgccg ttgccgttca ccaaagggct gcgtctgggc aacatgccgc agatccgcgt
    57961 gattgtggat gaagagctgg agagcgtgtg gaccggtaag aagacaccac agcaggcact
    58021 ggataccgcc gttgagcgtg gaaatcagtt gctgcgccgc tttgagaaat cgacgaagtc
    58081 ttaatcagtg taatgtcgga tgcgtttcgc ttatctgacc tggcatcgcg tgtaggccgg
    58141 ataagcgaag cgcatccggc acagttcagg aattaaccgt aatgtcatca tcccgtccgg
    58201 tgttccgctc gcgctggctg ccttatctgc tggtcgcgcc gcagctcatc atcaccgtta
    58261 tcttttttat ctggcctgcg ggcgaagcgt tgtggtactc gctgcaaagc gtcgatccgt
    58321 ttggtttctc cagccggttt gtcggcctgg ataacttcgt cgcgctgttt catgacagct
    58381 actatatcga ctccttctgg acgacgataa aattcagcac ctttgtcacc gtcagcggtt
    58441 tgctggtatc gctgttcttt gcggcgctgg tggagtacat cgtgcgcggc agtcgtttct
    58501 atcaaacctt gatgctgctg ccgtatgccg tggctcccgc cgttgccgcc gtattgtgga
    58561 tcttcctgtt taaccccggt cgcgggctga tcacccattt tctcgctgag ttcggctacg
    58621 actggaacca cgcgcaaaac agcggtcagg caatgtttct ggtggtgttt gcctcagtat
    58681 ggaagcaaat cagctacaac ttcctgttct tctatgccgc gctgcaatcc attccccgtt
    58741 cgttgatcga agccgcagcc atcgacggtg cagggccgat tcgccgcttc tttaagattg
    58801 cgctgccgct tatcgccccg gtgagtttct tcctgctggt agtgaacctg gtgtatgcct
    58861 tcttcgacac cttcccggtg atcgacgccg ccacgtccgg cgggccggtt caggccacca
    58921 ctacgctgat ttataagatc taccgcgaag gctttaccgg actggatctg gcttcgtctg
    58981 ccgcacagtc ggtggtgttg atgttcctcg tcatcgtgct gacggtgatg cagttccgct
    59041 atgttgaaag caaggtgcgt taccaatgat tgagaaccgt ccgtggctga cgatattcag
    59101 ccataccatg ctgatcctcg ggatcgcggt gatcctattc ccgctgtacg tggcgtttgt
    59161 cgcggcgacg ctggataaac aggccgtcta cgccgcgccg atgacgctca tccccggcac
    59221 gcatctgctg gaaaacatcc acaacatctg ggtaaacggg gtaggcacaa atagtgcgcc
    59281 gttctggcgg atgttgctta acagcttcgt gatggcgttc agcattacgc tcggcaaaat
    59341 taccgtctcg atgctctcgg cattcgccat tgtctggttt cgttttccgc tgcgtaacct
    59401 ctttttctgg atgattttta tcaccctgat gttgccggtt gaagtacgta tcttcccgac
    59461 ggtggaagtc atcgccaacc tgaagatgct cgacagctac gcaggtttaa cgctgccgct
    59521 gatggcctcg gcgaccgcga cgtttctttt ccgccagttc tttatgaccc taccggatga
    59581 actggtggaa gccgcgcgga tcgacggcgc gtcgccaatg cgcttctttt gcgacatcgt
    59641 ttttccgttg tccaaaacta atctggcggc gttgtttgtg atcaccttta tttacggctg
    59701 gaaccagtat ttgtggccgt tgttgattat taccgacgtg gatctcggca ccaccgtggc
    59761 agggatcaaa gggatgatcg ccactggcga aggcaccaca gaatggaact cagtgatggc
    59821 ggcgatgttg ttaaccctga tcccaccagt agtgattgtt ttagtgatgc agcgtgcctt
    59881 cgtgcgcggc ctggtcgata gtgagaaata agatggcagg actgaaatta caggcagtaa
    59941 ccaaaagctg ggatggcaaa acccaggtga ttaaaccgct gacccttgat gtggcggatg
    60001 gcgaatttat cgtgatggtc gggccgtcag gctgcgggaa atcgacgctg ctgcgcatgg
    60061 ttgccgggct ggagcgggtg acggaaggcg atatctgtat caacgaccag cgggtgaccg
    60121 aaatggagcc gaaagatcgc gggattgcga tggtgttcca gaactacgcg ctttatccgc
    60181 atatgagtgt tgaagaaaac atggcgtggg ggctgaaaat tcgcggcatg ggcaagcagc
    60241 aaattgccga gcgcgttaaa gaggcggcgc gcattctgga actggacggt ctgcttaagc
    60301 gccgcccgcg cgagctttcc ggcggtcagc gtcagcgtgt ggcgatgggc cgggcgattg
    60361 tgcgcgatcc ggcggtgttc ctgtttgatg agccactctc taacctcgat gccaagctgc
    60421 gcgtacagat gcgtcttgaa ctgcaacagc tgcaccgtcg cctgaaaacg acttcactct
    60481 acgttactca cgatcaggtt gaggcgatga ccctcgccca gcgagtaatg gtgatgaacg
    60541 gcggcgttgc cgaacagatt ggcacaccag ttgaagtcta cgaaaagccc gccagcctgt
    60601 ttgtggcgag ttttattggc agcccggcga tgaatctgct gacaggccgc gtgaataacg
    60661 aaggcacgca cttcgagctg gacggcggca ttgcgctgcc gctaaacggt ggctaccgtc
    60721 agtatgcagg gcgtaaaatg actctcggca ttcgcccgga acatatcgcg ctaagctcgc
    60781 aggcagaagg cggcgtgccg ctggtgatgg acacgctgga gatcctcggc gcagataacc
    60841 tggcgcacgg acgctggggc gaacagaagc tggtggtacg gctggcgcat caggagcgcc
    60901 cgacggcagg cagcacgctg tggctgcatc tgccggaaaa tcagctacat ctttttgatg
    60961 gtgaaacagg acaacgagta tgagtaactg gccttatccc cgcatcgtcg cccaccgtgg
    61021 cggcggtaaa ctggccccgg aaaacaccct ggcggcaatc gacgtcgggg caaaatacgg
    61081 tcataagatg atcgaatttg acgcgaagtt gtcgaaagat ggcgaaatct tcctgctcca
    61141 tgacgacaat ctcgaacgta ccagcaacgg ctggggcgtc gcgggtgaac tgaactggca
    61201 ggatttacta cgtgtggatg caggcagttg gtacggcaaa gcgtttaaag gtgagccgct
    61261 gccgttgctt tcgcaggtgg cggaacgctg tcgcgaacat gggatgatgg cgaatatcga
    61321 aatcaaaccc accactggca ctgggccatt aacgggcaaa atggtggcgc tgtcggcacg
    61381 cgagctgtgg gccggtatga cgccgccgct actgtcatcg tttgagattg atgctttaga
    61441 agccgcacaa caggcagtac cagaactgcc gcgcggtttg ttgctggatg agtggcgcga
    61501 cgactggcga gaactgaccg cgcggctggg ctgcgtctct attcatctca atcataagtt
    61561 gctcgataaa gcgcgagtga tgcagttgaa agacgccgga ttacggattc tggtttatac
    61621 cgtcaataaa ccccagcgcg cggcagagtt gctgcgctgg ggcgtggatt gcatctgtac
    61681 cgatgcgatt gatgtgattg gtccgaactt tacggcccaa tagttttcaa cggtatatca
    61741 ggctgcggcg tgccgggctg attcagcatg tcgccgtttc tcctctccgg caacatatgc
    61801 tgctgattca acgaactatc cggattacgg ttgctgttta acatcccgcc attggtgttg
    61861 ggcagcattt gctgccgggc gggatttcgc tcccccggct gtgactgcaa cacccgctga
    61921 gaattattgt ttatctggtt ttctaaatgc tgttgttgca actgagtttg cgttttcagt
    61981 tgctgattca gcatcccttt ttgctggatt tgctgcgttt gcatctgggt ttgcatccgc
    62041 tgctgactgg ggatctgata ccccggctgg ttagggttgt tcagagtatt aatgggctgt
    62101 gcaaagccga caaacggcag gagtgccgta agaatcagaa gtcgtttcat cgcgtatcct
    62161 cctctgaaga tattctttaa gtttactcct ttcctgcaat catcaggatt aattcagagt
    62221 tatataccag gcttagctgg ggttgcccct taatctctgg agaataacga tgataaaacc
    62281 gacgttttta cgccgggtgg ccattgctgc tctgttcaca ggaagttgtt ttagtacggt
    62341 agccgcgcca cctgcgccgt ctcctgtttc ttacggcgtg gaggaagatg tctttcatcc
    62401 ggtacgcgcg aaacagggga tggtggcatc tgtagatgct actgccactc aggtgggggt
    62461 ggatatcctc aaggagggcg ggaatgccgt tgatgccgcc gtggctgtgg gctacgcgct
    62521 ggcggtaacg catccgcagg cagggaatct gggcggtggt ggttttatgc tgatccgctc
    62581 gaaaaatggc aataccaccg ctatcgattt ccgcgaaatg gcacccgcca aagcgacccg
    62641 cgatatgttc cttgatgacc agggcaaccc ggacagcaaa aaatcactca cttcgcatct
    62701 ggcttccggc acgcccggta cggtcgctgg tttctcgctg gcgttggata aatacggcac
    62761 aatgccgttg aacaaagtgg tgcagccagc gtttaaactg gcacgcgatg gttttatcgt
    62821 taacgacgag ctggctgacg atctcaaaac ctacgggagc gaagtgctgc cgaatcacga
    62881 aaacagcaag gctatcttct ggaaagaggg cgagccgctg aaaaagggcg acaagctggt
    62941 gcaggcgaac ctggcaaaga gcctggagat gattgccgaa aacggcccgg acgaattcta
    63001 caaaggcacg atagcggaac agattgccca ggagatgcag aaaaacggtg gcctgatcac
    63061 taaagaagat ttagcggcgt ataaagcggt cgaacgcaca ccgataagcg gcgattatcg
    63121 cgggtatcag gtttactcca tgccaccgcc atcctccggc gggatccata tcgtacaaat
    63181 cctcaatatt ctggaaaact tcgatatgaa gaaatacggt tttggcagcg ccgacgcgat
    63241 gcagatcatg gcagaagcgg agaaatatgc ctacgctgac cgctcggaat atcttggcga
    63301 cccggatttt gtgaaagtcc cgtggcaggc gctgaccaat aaagcctatg ccaaatctat
    63361 tgccgagcaa atcgatatca ataaagcgaa accgtccagc gagattcgcc ccggcaagct
    63421 tgcgccttat gagagtaatc aaactaccca ttattcggtg gtggataaag acggtaacgc
    63481 ggtggcggtg acctatacgc tgaacaccac cttcggtacg ggtattgtcg cgggcgagag
    63541 cggtattttg ctcaataacc aaatggatga tttctctgcc aaaccgggcg taccgaacgt
    63601 ttacgggctg gtgggcggtg atgccaacgc cgtcgggccg aacaaacgcc cgctgtcatc
    63661 gatgtcacca accattgtgg tgaaagacgg taaaacctgg ctggtcaccg gtagccctgg
    63721 cgggagcctg atcatcacta cagtgcttca aatggtggtg aatagcatcg attatggcat
    63781 gaacgtcgcc gaagcgacca atgcgccgcg tttccaccat cagtggttgc cggacgagct
    63841 gcgtgtcgaa aaagggttta gcccggatac gctcaagctg ctggaagcaa aaggtcagaa
    63901 agtggcgctg aaagaggcga tgggcagtac acaaagcatt atggttgggc cggacggtga
    63961 gttgtactgc gcatccgacc cgcgctcggt ggatgattta acggcggggt actaaggtta
    64021 gcggccctct tcgtgggaag agggctgttt tcttgaacaa gataaattag tgtctggatt
    64081 gcttttacta ttgctccgta atatataatc ataattatcc tgaatgcttt taaggggctt
//
LOCUS       DBFIFM010000006        56055 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.006, whole genome shotgun sequence.
ACCESSION   DBFIFM010000006 DBFIFM010000000
VERSION     DBFIFM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.006"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..352
                     /gene="cmtB"
                     /locus_tag="N7C74_000337"
     CDS             <1..352
                     /gene="cmtB"
                     /locus_tag="N7C74_000337"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HGU2430282.1"
                     /translation="LLDKNYISENYIQAIKDSTINNGPYYILAPGVAMPHARPECGAL
                     KTGMSLTLLEQGVYFPGNDEPIKLLIGLSAADADSHIGAIQALSELLCEEEILEQLLT
                     ASSEKQLADIISRG"
     gene            380..1768
                     /gene="cmtA"
                     /locus_tag="N7C74_000338"
     CDS             380..1768
                     /gene="cmtA"
                     /locus_tag="N7C74_000338"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HGU2430283.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1783..3060
                     /gene="yggP"
                     /locus_tag="N7C74_000339"
     CDS             1783..3060
                     /gene="yggP"
                     /locus_tag="N7C74_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGU2430284.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDAGFDDIFVYAAVSAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3057..4022
                     /gene="glpX"
                     /locus_tag="N7C74_000340"
     CDS             3057..4022
                     /gene="glpX"
                     /locus_tag="N7C74_000340"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HGU2430285.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRDVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGNMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDVLVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLY"
     gene            4044..4553
                     /gene="fumE"
                     /locus_tag="N7C74_000341"
     CDS             4044..4553
                     /gene="fumE"
                     /locus_tag="N7C74_000341"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HGU2430286.1"
                     /translation="MATLSEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEESVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLKEQD
                     ETLGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4550..5263
                     /locus_tag="N7C74_000342"
     CDS             4550..5263
                     /locus_tag="N7C74_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HGU2430287.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWAYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPIIIVEGNWLLLDDDN
                     WRQLAQFCDFSIFINAPATALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5235..5912)
                     /locus_tag="N7C74_000343"
     CDS             complement(5235..5912)
                     /locus_tag="N7C74_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2430288.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNIN
                     PPDDIHP"
     gene            complement(5906..6583)
                     /locus_tag="N7C74_000344"
     CDS             complement(5906..6583)
                     /locus_tag="N7C74_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2430289.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6571..7278)
                     /locus_tag="N7C74_000345"
     CDS             complement(6571..7278)
                     /locus_tag="N7C74_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HGU2430290.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALTIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(7279..7857)
                     /locus_tag="N7C74_000346"
     CDS             complement(7279..7857)
                     /locus_tag="N7C74_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGU2430291.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKTLTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(7880..8311)
                     /locus_tag="N7C74_000347"
     CDS             complement(7880..8311)
                     /locus_tag="N7C74_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HGU2430292.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDIALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8683..9702
                     /gene="epd"
                     /locus_tag="N7C74_000348"
     CDS             8683..9702
                     /gene="epd"
                     /locus_tag="N7C74_000348"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HGU2430293.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9752..10915
                     /gene="pgk"
                     /locus_tag="N7C74_000349"
     CDS             9752..10915
                     /gene="pgk"
                     /locus_tag="N7C74_000349"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HGU2430294.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDEILSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11130..12209
                     /gene="fbaA"
                     /locus_tag="N7C74_000350"
     CDS             11130..12209
                     /gene="fbaA"
                     /locus_tag="N7C74_000350"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HGU2430295.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(12273..12371)
                     /locus_tag="N7C74_000351"
                     /pseudo
     CDS             complement(12273..12371)
                     /locus_tag="N7C74_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12567..13427
                     /gene="mscS"
                     /locus_tag="N7C74_000352"
     CDS             12567..13427
                     /gene="mscS"
                     /locus_tag="N7C74_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HGU2430296.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13566..14201
                     /gene="argO"
                     /locus_tag="N7C74_000353"
     CDS             13566..14201
                     /gene="argO"
                     /locus_tag="N7C74_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HGU2430297.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14294..15034
                     /gene="yggE"
                     /locus_tag="N7C74_000354"
     CDS             14294..15034
                     /gene="yggE"
                     /locus_tag="N7C74_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HGU2430298.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15201..16097
                     /gene="ygfI"
                     /locus_tag="N7C74_000355"
                     /pseudo
     CDS             15201..16097
                     /gene="ygfI"
                     /locus_tag="N7C74_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(16094..17572)
                     /gene="scpC"
                     /locus_tag="N7C74_000356"
     CDS             complement(16094..17572)
                     /gene="scpC"
                     /locus_tag="N7C74_000356"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HGU2430299.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHNEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17596..18381)
                     /gene="scpB"
                     /locus_tag="N7C74_000357"
     CDS             complement(17596..18381)
                     /gene="scpB"
                     /locus_tag="N7C74_000357"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HGU2430300.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18392..19387)
                     /gene="meaB"
                     /locus_tag="N7C74_000358"
     CDS             complement(18392..19387)
                     /gene="meaB"
                     /locus_tag="N7C74_000358"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HGU2430301.1"
                     /translation="MINEATLAESIRRLRQGEHATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19380..21524)
                     /gene="scpA"
                     /locus_tag="N7C74_000359"
     CDS             complement(19380..21524)
                     /gene="scpA"
                     /locus_tag="N7C74_000359"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HGU2430302.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKTGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIIAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGLDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(21728..22621)
                     /gene="argP"
                     /locus_tag="N7C74_000360"
     CDS             complement(21728..22621)
                     /gene="argP"
                     /locus_tag="N7C74_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HGU2430303.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            23004..23663
                     /gene="rpiA"
                     /locus_tag="N7C74_000361"
     CDS             23004..23663
                     /gene="rpiA"
                     /locus_tag="N7C74_000361"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HGU2430304.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            23918..25150
                     /gene="serA"
                     /locus_tag="N7C74_000362"
     CDS             23918..25150
                     /gene="serA"
                     /locus_tag="N7C74_000362"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HGU2430305.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            25379..25438
                     /gene="ibsC"
                     /locus_tag="N7C74_000363"
     CDS             25379..25438
                     /gene="ibsC"
                     /locus_tag="N7C74_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HGU2430306.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            25772..25831
                     /locus_tag="N7C74_000364"
     CDS             25772..25831
                     /locus_tag="N7C74_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU2430307.1"
                     /translation="MMRLVIILIVLLLISLPAY"
     gene            complement(25932..26534)
                     /gene="fau"
                     /locus_tag="N7C74_000365"
     CDS             complement(25932..26534)
                     /gene="fau"
                     /locus_tag="N7C74_000365"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HGU2430308.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(26555..26738)
                     /gene="ssrS"
                     /locus_tag="N7C74_000366"
     ncRNA           complement(26555..26738)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="N7C74_000366"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(26780..27109)
                     /gene="zapA"
                     /locus_tag="N7C74_000367"
     CDS             complement(26780..27109)
                     /gene="zapA"
                     /locus_tag="N7C74_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGU2430309.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            27277..27855
                     /gene="ygfB"
                     /locus_tag="N7C74_000368"
     CDS             27277..27855
                     /gene="ygfB"
                     /locus_tag="N7C74_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HGU2430310.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            27881..29206
                     /gene="pepP"
                     /locus_tag="N7C74_000369"
     CDS             27881..29206
                     /gene="pepP"
                     /locus_tag="N7C74_000369"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HGU2430311.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DIDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            29203..30381
                     /gene="ubiH"
                     /locus_tag="N7C74_000370"
                     /gene_synonym="visB"
     CDS             29203..30381
                     /gene="ubiH"
                     /locus_tag="N7C74_000370"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HGU2430312.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLAHCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLEGGETLTGRVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARS
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            30405..31607
                     /gene="ubiI"
                     /locus_tag="N7C74_000371"
                     /gene_synonym="visC"
     CDS             30405..31607
                     /gene="ubiI"
                     /locus_tag="N7C74_000371"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HGU2430313.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPVKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(31681..31764)
                     /locus_tag="N7C74_000372"
     CDS             complement(31681..31764)
                     /locus_tag="N7C74_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2430314.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
     gene            32055..33149
                     /gene="gcvT"
                     /locus_tag="N7C74_000373"
     CDS             32055..33149
                     /gene="gcvT"
                     /locus_tag="N7C74_000373"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HGU2430315.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHEVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEGGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPKGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            33173..33562
                     /gene="gcvH"
                     /locus_tag="N7C74_000374"
     CDS             33173..33562
                     /gene="gcvH"
                     /locus_tag="N7C74_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HGU2430316.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            33680..36553
                     /gene="gcvP"
                     /locus_tag="N7C74_000375"
     CDS             33680..36553
                     /gene="gcvP"
                     /locus_tag="N7C74_000375"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HGU2430317.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDIFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(36719..38152)
                     /gene="bglA"
                     /locus_tag="N7C74_000376"
     CDS             complement(36719..38152)
                     /gene="bglA"
                     /locus_tag="N7C74_000376"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HGU2430318.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINLEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDSDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            38197..38508
                     /gene="yqfB"
                     /locus_tag="N7C74_000377"
     CDS             38197..38508
                     /gene="yqfB"
                     /locus_tag="N7C74_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HGU2430319.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            38672..39331
                     /gene="yqfA"
                     /locus_tag="N7C74_000378"
     CDS             38672..39331
                     /gene="yqfA"
                     /locus_tag="N7C74_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HGU2430320.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(39408..40388)
                     /gene="ygfZ"
                     /locus_tag="N7C74_000379"
     CDS             complement(39408..40388)
                     /gene="ygfZ"
                     /locus_tag="N7C74_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HGU2430321.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYLQGQ
                     VTADVSQMTDDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLAELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLCIEPLPYSLEE"
     gene            40631..40897
                     /gene="sdhE"
                     /locus_tag="N7C74_000380"
     CDS             40631..40897
                     /gene="sdhE"
                     /locus_tag="N7C74_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HGU2430322.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            40878..41285
                     /gene="ygfX"
                     /locus_tag="N7C74_000381"
     CDS             40878..41285
                     /gene="ygfX"
                     /locus_tag="N7C74_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HGU2430323.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(41325..41846)
                     /gene="fldB"
                     /locus_tag="N7C74_000382"
     CDS             complement(41325..41846)
                     /gene="fldB"
                     /locus_tag="N7C74_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HGU2430324.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            41958..42854
                     /gene="xerD"
                     /locus_tag="N7C74_000383"
     CDS             41958..42854
                     /gene="xerD"
                     /locus_tag="N7C74_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HGU2430325.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            42879..43589
                     /gene="dsbC"
                     /locus_tag="N7C74_000384"
     CDS             42879..43589
                     /gene="dsbC"
                     /locus_tag="N7C74_000384"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HGU2430326.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            43595..45328
                     /gene="recJ"
                     /locus_tag="N7C74_000385"
     CDS             43595..45328
                     /gene="recJ"
                     /locus_tag="N7C74_000385"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HGU2430327.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            45419..46517
                     /gene="prfB"
                     /locus_tag="N7C74_000386"
     CDS             join(45419..45493,45495..46517)
                     /gene="prfB"
                     /locus_tag="N7C74_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HGU2430328.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            46527..48044
                     /gene="lysS"
                     /locus_tag="N7C74_000387"
     CDS             46527..48044
                     /gene="lysS"
                     /locus_tag="N7C74_000387"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGU2430329.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIVESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(48087..48635)
                     /gene="idi"
                     /locus_tag="N7C74_000388"
     CDS             complement(48087..48635)
                     /gene="idi"
                     /locus_tag="N7C74_000388"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HGU2430330.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            48690..48761
                     /locus_tag="N7C74_000389"
     CDS             48690..48761
                     /locus_tag="N7C74_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2430331.1"
                     /translation="MINQASVYRQPPVLSGCRQVKTI"
     gene            complement(48758..48883)
                     /gene="yqfG"
                     /locus_tag="N7C74_000390"
     CDS             complement(48758..48883)
                     /gene="yqfG"
                     /locus_tag="N7C74_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HGU2430332.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(48885..50333)
                     /gene="uacT"
                     /locus_tag="N7C74_000391"
     CDS             complement(48885..50333)
                     /gene="uacT"
                     /locus_tag="N7C74_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HGU2430333.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAQLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            50769..52688
                     /gene="ygfT"
                     /locus_tag="N7C74_000392"
     CDS             50769..52688
                     /gene="ygfT"
                     /locus_tag="N7C74_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HGU2430334.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGLAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKNHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            52688..53176
                     /gene="ygfS"
                     /locus_tag="N7C74_000393"
                     /pseudo
     CDS             52688..53176
                     /gene="ygfS"
                     /locus_tag="N7C74_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
     gene            complement(53212..54579)
                     /gene="ghxQ"
                     /locus_tag="N7C74_000394"
                     /gene_synonym="ygfQ"
     CDS             complement(53212..54579)
                     /gene="ghxQ"
                     /locus_tag="N7C74_000394"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HGU2430335.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(54615..55931)
                     /gene="guaD"
                     /locus_tag="N7C74_000395"
     CDS             complement(54615..55931)
                     /gene="guaD"
                     /locus_tag="N7C74_000395"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HGU2430336.1"
                     /translation="MSGEHTLKAVRGSFIDVARTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLKEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(55949..>56055)
                     /locus_tag="N7C74_000396"
     CDS             complement(55949..>56055)
                     /locus_tag="N7C74_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2430337.1"
                     /translation="CAGGLTAILLNIILPGGYRQENVLPGITSAEEMD"
BASE COUNT        13574 a        13984 c        14999 g        13498 t
ORIGIN      
        1 attgctggat aaaaactata tcagcgagaa ttacattcag gcaattaaag attccaccat
       61 taacaatggc ccttattata ttctcgcacc aggcgtggca atgcctcatg cgcgaccgga
      121 atgtggggcg cttaaaaccg ggatgtcttt gacattactt gaacaaggtg tttattttcc
      181 ggggaatgac gaaccaatta aattactcat cggactctct gctgccgatg ccgattcgca
      241 cattggcgct attcaggcgt taagtgaatt actgtgcgaa gaagaaatac tcgaacaact
      301 cttaacagca tcatcagaaa aacaattagc ggacattatc agccgcggat aattactctc
      361 tcctctacag gataatatta tggaaaacaa gtctgctcgt gcaaaggtcc aggcttttgg
      421 gggctttttg actgcaatgg tcatccccaa tattggtgct tttattgcct ggggttttat
      481 tactgcgtta tttattccca ccggttggct gcctaacgaa catttcgcca aaattgtcgg
      541 cccgatgatt acctatttat tgcccgtgat gattggttct accggtggtc atctggtcgg
      601 cggtaaacgc ggcgcggtca tgggcggaat aggtactatt ggtgtgatcg tcggcgcaga
      661 gatcccgatg ttccttggct caatgattat ggggccgctc ggtggtctgg tcataaaata
      721 tgttgataag gcactggaaa aacgcatacc tgccggtttt gagatggtta tcaataactt
      781 ctcattaggt atcgcgggga tgctcctttg cctgctgggt tttgaagtga tcggcccggc
      841 ggtgttaatt gccaatactt tcgtcaaaga gtgtattgag gcgctggtac atgcgggtta
      901 tctgcctctg ctgtcagtca tcaatgaacc ggcgaaagtg cttttcctta ataatgcgat
      961 cgatcagggc gtctattatc cgctgggaat gcaacaggct tcggttaacg gtaaatccat
     1021 cttctttatg gtggcctcta acccaggtcc gggcctgggg ctgctgctgg cgtttacctt
     1081 gtttggtaaa gggatgagta aacgttctgc gcccggggcg atgattattc acttcctcgg
     1141 tgggatccac gaactgtatt tcccatatgt gctgatgaag ccgctgacta ttattgccat
     1201 gattgcgggc ggtatgtctg gcacctggat gtttaactta ctggacggtg gtctggtggc
     1261 tggcccaagt ccggggtcta tctttgctta cctggcgctg acgccgaaag gttcgttcct
     1321 ggcgacaatt gccggtgtta cggtaggtac cctggtgtcc tttgctatca cttcgctgat
     1381 actgaagatg gaaaaaacgg tggaaacgga gagcgaagat gagtttgctc agtcagccaa
     1441 tgcggttaag gcgatgaaac aagagggtgc attctcgtta agcagggtta agcgtatcgc
     1501 ctttgtttgc gatgcgggga tgggctccag tgcgatgggc gcgaccacct tccgtaaacg
     1561 cctggaaaaa gcggggctgg caattgaagt aaaacattac gccatagaaa acgtgcctgc
     1621 ggatgcggat atcgtcgtta ctcatgccag tctggaaggg cgcgtgaaac gtgtgacgga
     1681 taaaccactg atattgatta ataactatat tggcgatcca aaactcgaca ctttatttaa
     1741 tcaattaacc gccgaacata aacactgatt acagaggtaa aaatgaaaac caaagttgct
     1801 gctatttatg gcaagcggga tgtccgtctg cgcgaatttg aactgccaga aattaccgat
     1861 aatgaattac tggtgagtgt gatttctgac agcgtctgtt tatccacctg gaaagcggcg
     1921 ttactcggta gtgaacataa acgcgtaccc gacgatttag aaaatcatcc ggtcattacc
     1981 gggcatgaat gtgccggggt tattgtcgaa gtgggtaaaa atctcactgg caaatataaa
     2041 aaaggccagc gttttgtatt gcaaccggcg atggggttac caagcggata ttcagcgggc
     2101 tacagctacg aatattttgg cggcaatgcc acttatatga ttattcccga aatcgccatt
     2161 aatttgggct gcgtattacc gtatcacggc tcttattttg ctgcggcgtc gctggcagag
     2221 cctatgtgct gcattattgg cgcttatcat gccaattatc acaccacgca atatgtttat
     2281 gagcatcgca tgggcgtcaa acctggcggc aatattgcac tgctggcgtg tgcaggtccg
     2341 atgggcattg gcgctatcga ttacgccatt aacggcggca tacaaccgtc gcgggtggtg
     2401 gtggtcgata tcgacgacaa acgtctggcg caggtacaga agctgctgcc ggtggaactg
     2461 gcggccagta aaggcattga gctggtgtat gtgaatacca aagggatgag cgatcctgtc
     2521 cagatgctgc gggcgctgac aggagatgcc gggttcgatg acatttttgt ttatgcggcg
     2581 gtgtctgctg tcgttgagat ggctgatgaa ttactggcgg aagatggctg tctgaacttc
     2641 tttgccgggc cgacggataa aaacttcaaa gtgccgttta atttctacaa cgttcattac
     2701 aacagcacgc acgtcgtcgg tacatccggc ggttcaacgg acgacatgaa agaggcgatt
     2761 gcccttagcg ccacagggca gttacagccc tcgtttatgg tgacccatat cggtggcctg
     2821 gatgcggtgc cagaaaccgt gctcaatctg ccggatatcc ctggcggtaa aaaactcatt
     2881 tataacggcg tgaccatgcc gctcactgcc attgccgatt ttgccgaaaa aggcaaaacc
     2941 gatccgctgt ttaaagagtt ggcgcggctg gttgaggaaa cgcacggcat ctggaatgaa
     3001 caggccgaga aatatctgct ggcacaattt ggcgttgata tcggggaggc cgcgcaatga
     3061 tgtccctggc gtggccatta tttcgcgtta cggaacaggc agcgttggct gcctggccgc
     3121 aaaccggatg tggcgacaaa aataaaattg atggcctggc ggtcaccgcg atgcgccagg
     3181 cattaaacga cgtcgctttt cgtggacgag tggttatcgg cgaaggagaa attgaccatg
     3241 cgccgatgct gtggattggc gaagaggtgg gcaaaggcga tgggccagaa gtcgatatcg
     3301 cggttgatcc cattgaaggc acgcggatgg tggcgatggg gcagagcaat gcgctggcgg
     3361 tcatggcttt cgcccctcga gatagcctgt tacatgcgcc cgatatgtat atgaaaaagc
     3421 tggttgttaa tcgactggct gccggggcga ttgatctgtc tttgcctctg gcggacaact
     3481 tgcgtgacgt ggcgcgggcg ttaggtaaac cgctggacaa gctgcgcatg gttacgctgg
     3541 ataaaccgcg tctgagtgcc gcgattgaag aagcaacaca gctgggcgtg aaggtttttg
     3601 ccctgccgga tggcgatgtc gccgccagcg tgctgacctg ctggcaggat aatccttacg
     3661 atgtgatgta caccatcggc ggtgcgccgg aaggcgtgat ttctgcctgt gccgttaaag
     3721 cattaggcgg gaatatgcag gccgagctga ttgacttttg tcaggccaaa ggggattaca
     3781 cggaaaatcg gcagattgca gagcaggagc gcaagcgttg caaggcaatg ggtgttgacg
     3841 ttaaccgtgt gtactcgctc gatgtactgg taagggggaa cgatatcctc tttagcgcta
     3901 cgggcgtcac gggcggcgag ttggtgaacg gtatccaaca gacggcgaat ggagtgcgga
     3961 cgcagacatt actgatcggc ggcgcggacc aaacgtgtaa tataatagac tccctgtatt
     4021 gatggtgatc tatcggaaga ccaatggcga cgctgtcaga agatgatgtg cttgagcaac
     4081 tggatgcaca ggacaattta ttttcattta tgaaaactgc gcattctatt ttgctccagg
     4141 ggatacgcca gtttctgccg tcgctgtttg tcgataacga tgaagagagc gtcgaatatg
     4201 cagtgaagcc gttactcgcc caaagcggcc cgcttgacga tattgatgtt gcactgcgtt
     4261 tgatttatgc gctggggaaa atggataaat ggctgtacgc cgatatcacg catttttccc
     4321 agtactggca ttacctgaaa gaacaggatg aaacgctcgg atttgccgat gacatgactt
     4381 gggattttat cagcaacgtc aatagcataa cctgcaatgc gacgctctat gacgcgttga
     4441 aagcgatgaa gttcgccgat ttcgccgtct ggtcagaggc gcgttttagc ggaatggtca
     4501 aaacggcgct gacgctggca gtaacgacca ccttaaagga attaacgccg tgaaaattga
     4561 attaacggtg aatgggctga acgtgcaagc gcagtaccat gatgaagaga tagaacgcgt
     4621 gcataaaccg ttgctgcgta tgctggcggc cttacagacg gtaaatccgc agcgccgaac
     4681 cgtggttttt ctttgcgctc cgccggggac gggtaaatct accctaacca ccttctgggc
     4741 atatctcgcg caacaagacc cggagctgcc cgctattcag acgctcccga tggatggttt
     4801 tcaccattac aatagctggc tggatgcgca tcaattgcgc cccttcaaag gcgcaccaga
     4861 gacatttgac gttgcgaaac tggcggaaaa tctgcgccag gtcgtggaag gggattgtac
     4921 gtggccgcag tacgatcgac aaaagcatga tcctgttgaa gatgcgttgc acgttaccgc
     4981 accaataatc atcgtcgaag gaaactggct attgctggat gacgataact ggcgccagct
     5041 agcgcaattt tgtgatttct cgatctttat caatgcgcca gctactgcgt tgcgggaacg
     5101 actggtgggg cgcaagctgg cgggagggtt atcgctggca gacgcagaag ctttttatga
     5161 ccgtaccgac gggccgaatg ttcgccgggt gctggaagag agtctgcctg caaatttgac
     5221 cttaatgatg accgctacgg gtgaatatcg tctggtggat taatgttcct gattagacca
     5281 tcttccagtc gcaccacacg agagaaatga cgccgggtaa atgcggcgtc atgactgatt
     5341 gctacgacgc tcgtgcctcg ctggcggcac ttctccagcc agctttcaaa cacgctcaac
     5401 caattttcgt caaaatcccg actcggctcg tccagcagca atagtggcgg agagagtgct
     5461 tccaggcagg cgacggcgac catccgtcgc tgcgcactat gcagatccaa cgggtgcgcg
     5521 ctggcagtgt ctgctaactg acaacattgc agggcagcat gggtacgttg agtgatttca
     5581 tccgccgggc atttttgcag tttcaaacca aatgcgatct cgtcagcgac ggtgctgtga
     5641 aaaagttggt tttccgcttc ctgaaacagt acgccaatct ttgccgcgcg ctggcggttt
     5701 ttcaggtttt tcatcgcctg ttgttgcagc ataaccgtac cggctgtggg cgtaagaaga
     5761 cctgccatta ctcgtaacag cgtcgatttc cctgcgccat tgtcgccggt caacgccaac
     5821 cattcacctt gtttgagttg cagagaaatg tcgcaaagac aatccgttgc cgcgccaggc
     5881 cagcgataag atatctgatt taacgttaac atagtggctg gagcgctccg ttttgcaatt
     5941 gccacgcttg ctggcagtag ttcaggaaag gtgtgcgatg acgttcaaaa agaataatca
     6001 gcgaaccacg ttcaaacgcc cagtgttgta agcgctgcaa cagcatttcg ctggcctgcg
     6061 gcgtcaggcg gctaaaggct tcatcaagaa ttaatagctt tggctgcatc gcgatggcgc
     6121 aggcgatgac cacgcgctgt gtctctccac cggagagtgt tgccgggtga cgatggcgca
     6181 acggctggca ctcggtcaga gtcagggcgg catcaatccg cgccataatc tctgcttcag
     6241 caagacacag attttctggc ccaaaggcaa cttcttcttc cacgctaaag gtacagccgg
     6301 agagctgcaa atagggcgat tgctggacga gctggatggt ggcggattgt tcattaagcg
     6361 ggagatgccc aatgggcgta ccgaggagcg tccccgtgcc tgttatctct cctggcagaa
     6421 agtccggata ccagcccgcc atcagctgtg ccagcgtact tttgccactg ccgttgtcac
     6481 caaagatggc gaccatcccc ggtttgacat agtgaaagtc ataacagaac ggaggatgcg
     6541 ttgagtgtgt cgggcaataa cggaactgtt ctaacgtaac catatccacg ctccgaattc
     6601 tgtcagcatc agcaagatca tggtgtaacg cgctacgcgt tgtagggtgc tatcggctgg
     6661 tgcccacaac gttgttcgtc ggttgtggat ccgaaatgcc cgcatatcca gcgccgcacc
     6721 acggacagca agatcgttta gcgcattgtg tgttagcgga atagttaacg cgggcatcgc
     6781 acgtaaacgc tgataccagc cctcatccag cggcacgccg cgggcacgct gcgcttcatg
     6841 aataatcgct aattgccgtt ttaactgctc aacgaccaat aacggcccgg caaacagata
     6901 agcgacgccc ggcggcaggc gagaagcaaa cagagcgcgg ataaaacatt gcacggggac
     6961 gtactgcatc cacaactgtg aggttgaaac aatcgccaga atccgcaacc agagcgtaat
     7021 agcatgcgtc caacgctccg gcgaacgcgg cgtgccgctt agccattctg ttagccagcc
     7081 accgtgtacc agccacagcc cggctcccag cgagaacatc agccagacga catattttgc
     7141 ccgccgccgc gtggccttta gcgcaataag gcaaaaaaag gtcgccgcgc tatagatcgg
     7201 caaaatggtc tgcgcgggca ggataagcgt ggtgcaggcc gccaatgccc atagtgttaa
     7261 tgaggtaaat ggatgcattt agcgcacggc agccattgcc ggaaaatggc gcgtggtgcg
     7321 aatcggcagc tggcgcagta aaagccagac aatcaccgct gtaagagttt tatcgaccag
     7381 gttcgcgcct atcaccgtaa tcgccaccga ctccaccagg ttttgcccca tcgagtgcat
     7441 ccaggcaacg aacaaatcgg ctccgctacc ggtgacaccg ccgaacagcg cagtacgcag
     7501 cggtacagcg acgaccgtta ccgcaagcgt aataatcacg ccgctgacaa cgacttttgg
     7561 cagggtgcga aaccaacccg ctcgcgccag ccaaccggcg accaggccga tgaccatcgc
     7621 gacgggggca aaagcggcag cgatcgggtc ggtcagtaat ccccagagta aattggtcag
     7681 taagccggtc agcatcccga tcaccggacc aagcagtacc gcgctaataa gtgtgccgat
     7741 ggagtcgaga aatataggta atttgaccat gctggccagt tgcccgccga tcatattgat
     7801 ggcgatagat ataacgatca acaccagtgc ctgactggaa aagtggctac gcgccataag
     7861 aaaacctctc tgaaagtagt tatttaacgg gggaaatatg caactcgtcg taaccagaaa
     7921 gcgcgcaagg ttggcggcta aacgccagtt cctcaaggct accgatcacc agttcaagcg
     7981 tggtccgcac ggtacagccg ggcatcatat gaccgccaag catcgtgccg tgtggatcgg
     8041 aaacgcagag atgaagatgc tcaccactct gttccaacgt gccgtttagt gagatcactt
     8101 cgaatgtacc gttcaggagc gtggtgcctt cttgcccggc atagcgcaag gcgatgtcag
     8161 tcaggctacc ggtacagcct gcaatccagg cggcatgaag ttgctgctgc tgtgcaaaag
     8221 cgtgtagctg ggaaaaaact tcctgcccgg ggaggaggcg cagtgcgtaa aagcgtgcgg
     8281 aggaagaata tgggtagggc aatgtcatca ttggagtact ccgaaactcg gacgtattac
     8341 ggataatatc gacgctgcat cagtcatatt ctgatataag tcagggtttg tgacggcaca
     8401 acgaaaagat tttgctacgc ttattcgtga ttctgcaaag tggaataaat tccacagggg
     8461 cggggctgaa aacgtgacgt aagtcagata attgttttct ttgactggac aatcattcct
     8521 tttattccac gtttcgctta tcctagctga agcgtttcag tcgattaaat gttcgacaat
     8581 taaccaatca gtcgcagttt gcgacaggta aggtttcccc ggacgatttg ctggattact
     8641 ctgtctggca aatttgctca gggaaaacct tgcaggagat ctatgaccgt acgcgtagcg
     8701 ataaatggct tcggtcgcat cgggcgtaat gtggttcgtg ctttgtatga atccggacgc
     8761 cgggcggaaa ttaccgtggt ggcaatcaac gaactggcgg atgctgcggg catggcgcat
     8821 ttgttgaaat acgacaccag ccatggccgt tttgcatggg aagtacgaca ggaacgcgat
     8881 caactttttg ttggtgatga cgccatccgc gtattgcatg aacgttcact gcaatcgctc
     8941 ccctggcgtg aacttggcgt tgatgtagtc ctcgactgca ccggcgtata tggctcccgc
     9001 gagcatggcg aagcgcacat tgccgccggg gctaaaaaag tgctcttttc acatcctggc
     9061 agtaacgatc tcgacgcgac cgttgtttac ggcgtcaatc aggatcaact tcgtgcggag
     9121 caccgcatcg tttctaacgc ttcctgtacc acgaattgca taattcccgt catcaaattg
     9181 ttagatgatg cgtacggtat tgagtccggc actgtgacca caattcactc cgccatgcac
     9241 gatcaacagg ttattgatgc ataccatcct gacctgcgtc gcacccgggc agccagccag
     9301 tcgatcattc cggtcgatac taaactggcc gccggtatca cacgattttt tccgcaattt
     9361 aacgatcgct ttgaagcgat tgcggtacgt gtgccaacca taaatgtgac ggcaatcgat
     9421 ttaagcgtga cggtgaagaa acctgtaaaa gccaatgaag tcaacctgtt gctgcaaaaa
     9481 gcagcacaag gtgcatttca tggtatagtt gactatacgg aattgccgtt ggtctctgta
     9541 gattttaacc acgatccgca cagtgccatt gtcgatggca cccaaacccg ggtcagtggc
     9601 gcacacctga tcaaaacgtt ggtctggtgc gataacgaat ggggctttgc taaccgaatg
     9661 ctcgacacga cgttagctat ggctactgtt gctttcaggt aagacgcaag cagcgtctgc
     9721 aaaactttta gaatcaacga gaggattcac catgtctgta attaagatga ccgatctgga
     9781 tcttgctggg aaacgtgtat ttatccgtgc ggatctgaac gtaccagtaa aagacgggaa
     9841 agtaaccagc gacgcgcgta tccgtgcttc tctgccgacc atcgaactgg ccctgaaaca
     9901 aggcgcaaaa gtgatggtaa cttcccacct gggtcgtcct accgaaggcg agtacaacga
     9961 agaattctct ctgctgccgg ttgttaacta cctgaaagac aaactgtcta acccggttcg
    10021 tctggttaaa gattacctcg acggcgttga cgttgctgaa ggtgaactgg ttgttctgga
    10081 aaacgttcgc ttcaacaaag gcgagaagaa agacgacgaa atcctgtcca aaaaatacgc
    10141 tgcactgtgt gacgtgttcg taatggacgc attcggtact gctcaccgcg cgcaggcttc
    10201 tactcacggt atcggtaaat tcgctgacgt tgcgtgcgca ggcccgctgc tggcagctga
    10261 actggacgcg ctgggtaagg cactgaaaga acctgctcgc ccgatggtgg ctatcgttgg
    10321 tggttctaaa gtatctacca aactgaccgt tctggactcc ctgtctaaaa tcgctgacca
    10381 gctgattgtt ggtggtggta tcgctaacac ctttatcgcg gcacaaggcc acgatgtggg
    10441 taaatccctg tacgaagctg acctggttga cgaagcaaaa cgtctgctga ccacctgcaa
    10501 catcccggtt ccgtctgatg ttcgcgtagc aaccgagttc tctgaaaccg ctccggctac
    10561 cctgaaatct gttaacgacg tgaaagctga cgagcagatc ctggatatcg gtgatgcttc
    10621 cgctcaggaa ctggctgaaa tcctgaagaa tgcgaaaacc attctgtgga acggtccggt
    10681 tggcgtgttc gaattcccga acttccgcaa aggtactgaa atcgtggcta acgctatcgc
    10741 agacagcgaa gcgttctcca tcgctggcgg cggcgacact ctggcagcaa tcgacctgtt
    10801 cggcattgct gacaaaatct cctacatctc cactggcggc ggcgcattcc tcgaattcgt
    10861 ggaaggtaaa gtactgcctg cagtagcgat gctcgaagag cgcgctaaga agtaaaaaat
    10921 cacagggcag ggaaacctgc ccttgtttca gcgcgctttt agagcacgca ttttttcata
    10981 ctctaaataa ttcgagttgc aggaaggcga caagagagtg aatccccagg agcttacata
    11041 agtaagtgac tggggtgagc gaacgcagac gcagcacatg caacttgaag tatgacgagt
    11101 ataaggcccg acgatacagg acaagagaca tgtctaagat ttttgatttc gtaaaacctg
    11161 gcgtaatcac tggtgatgac gtacagaaag ttttccaggt agcaaaagaa aacaacttcg
    11221 cactgccagc agtaaactgc gtcggtactg actccatcaa cgccgtactg gaaactgctg
    11281 ctaaagttaa agcgccggtt atcgttcagt tctccaacgg tggtgcttcc tttatcgctg
    11341 gtaaaggcgt gaaatctgac gttccgcagg gtgctgctat cctgggcgcg atctctggtg
    11401 cgcatcacgt tcaccagatg gctgaacatt atggtgttcc ggttatcctg cacactgacc
    11461 actgcgcgaa gaaactgctg ccgtggatcg acggtctgct ggacgcgggt gaaaaacact
    11521 tcgcagctac cggtaagccg ctgttctctt ctcacatgat cgacctgtct gaagaatctc
    11581 tgcaagagaa catcgaaatc tgctctaaat acctggagcg catgtccaaa atcggcatga
    11641 ctctggaaat cgaactgggt tgcaccggtg gtgaagaaga cggcgtggac aacagccaca
    11701 tggacgcttc tgcactgtac acccagccgg aagacgttga ttacgcatac accgaactga
    11761 gcaaaatcag cccgcgtttc accatcgcag cgtccttcgg taacgtacac ggtgtttaca
    11821 agccgggtaa cgtggttctg actccgacca tcctgcgtga ttctcaggaa tatgtttcta
    11881 agaaacacaa cctgccgcac aacagcctga acttcgtatt ccacggtggt tccggttcta
    11941 ctgctcagga aatcaaagac tccgtaagct acggcgtagt gaaaatgaac atcgataccg
    12001 atacccaatg ggcaacctgg gaaggcgttc tgaactacta caaagcgaac gaagcttatc
    12061 tgcagggtca gctgggtaac ccgaaaggcg aagatcagcc gaacaagaaa tactacgatc
    12121 cgcgcgtatg gctgcgtgcc ggtcagactt cgatgatcgc tcgtctggaa aaagcattcc
    12181 aggaactgaa cgcgatcgac gttctgtaag tcattccttt ctgcttatct caaggcccgc
    12241 tctgcgggtc tttttttcgc caaaagcaaa attgcctgat gcgctatgct tatcaggcct
    12301 acacgtatcc tgcaatatat tgaatttgca ggtttttgta ggccggataa ggcgttcacg
    12361 ccgcatccgg catgacaaag cgcacattga tatctgtctg agccccgctc tgcggggatt
    12421 tttttgccaa aagcaaggtg attcagatga gaaatctgtg atctatttgg caaaattatg
    12481 ctttattgtt tacccttgtc agactgcccg tcataaggcg gcggagtgta tttctccatt
    12541 ttgagtcagt tgaaaaggaa tattgaatgg aagatttgaa tgttgtcgat agcataaacg
    12601 gcgcgggaag ctggctggta gctaaccagg cgctgctgct aagttatgca gtaaacatcg
    12661 tggcggcact cgcgatcatc atcgttggtt tgattatcgc gcggatgatt tccaacgcgg
    12721 tgaatcgcct gatgatctcc cgtaaaatcg atgccactgt tgctgatttt ctttctgcat
    12781 tagtccgtta cggtattatc gcctttacgc taatcgcagc cctgggacgc gtgggtgtac
    12841 aaacagcgtc agtcattgct gtactcggtg ccgcaggctt agctgttggt ctggctttgc
    12901 aggggtcact ttctaacctg gccgctggcg tgttacttgt catgttccgc ccgttccgtg
    12961 ccggagaata tgttgacctc ggcggcgtag ccggtactgt gctgagtgtg cagattttct
    13021 ccaccaccat gcgtactgca gacggtaaaa ttatcgttat tccgaacggt aaaattattg
    13081 ccggaaatat tattaacttc tcccgcgagc cagttcgccg taacgaattt attattggcg
    13141 tggcgtatga ttccgatatc gatcaggtta agcagatcct gaccaatatt atccagtctg
    13201 aagatcgcat tttgaaagat cgcgaaatga ctgtgcgcct gaacgaactt ggtgcatcgt
    13261 cgattaattt cgtggtccgc gtctggagca acagcggcga tctgcaaaac gtgtactggg
    13321 atgtgctgga gcgtattaaa cgtgaatttg atgccgccgg tatcagcttc ccgtacccgc
    13381 aaatggatgt gaactttaag cgggtgaaag aagacaaagc tgcgtaatca acgctgaggc
    13441 cagataatac tctctggcct ctctcttatt agttattctg attgccaatt aatattatca
    13501 atttccgcta ataacaatcc cgcgatatag tctctgcatc agatacttaa ttcggaatat
    13561 ccaacgtgtt ttcttattac tttcaaggtc ttgctcttgg ggcagctatg attctgccgc
    13621 tcggcccaca aaatgctttt gtgatgaatc agggcattcg tcgtcagtac cacattatga
    13681 ttgccttact ttgcgctatc agcgatttgg tcctgatttg cgcggggatt tttggtggta
    13741 gcgcgttatt gatgcagtcg ccgtggttgc tggcgctggt cacctggggc ggcgtagtct
    13801 tcttgctgtg gtatggtttt ggcgctttta aaacagcgat gagtagtaat attgagttag
    13861 ctagtgccga agtcctgaag caaggcagat ggaaaattat cgccaccatg ttggcagtga
    13921 cctggctgaa tccgcatgtt tacctagata cttttgttgt actgggcagc cttggcgggc
    13981 agcttgacgt cgaaccaaaa cgttggtttg cgctcgggac aattagcgcc tctttcctgt
    14041 ggttctttgg tctggctatt ctcgcagcct ggctggcacc gcgcctgcgc acggcaaaat
    14101 cacagcgcat tatcaatctg gtagtgggat gtgtcatgtg gtttatagcc ttgcagctgg
    14161 cgagagacgg tattgctcat gcacaagcct tgttcagtta ggcgctatct gatggaaaaa
    14221 taaaacagag gcgctaagct tgcctccaga gatcctgaat tttgggcaag tcaattaaca
    14281 cggagagact aacgtgaagt tcaaagttat cgccctggcg gcattaatgg gtattagcgg
    14341 gatggcagcg caggctaacg aattgccgga tggaccgcat attgtcacct ccggtacggc
    14401 aagtgtggat gcggtgccag acattgccac tcttgcgatt gaagttaacg tggccgcgaa
    14461 ggatgccgct acagccaaga aacaggcaga tgagcgcgtc gcacaataca tttccttcct
    14521 tgaactcaat cagatcgcga aaaaagatat cagctcagcg aatttacgca cccagccaga
    14581 ttatgattat caggatggta aaagtatcct gaaaggctac cgcgctgtga gaacggtgga
    14641 agtcacgctc cgtcagttag acaaactgaa ttccttgctg gatggcgcgc tgaaggcggg
    14701 tcttaacgaa attcgttcag tgtcgctggg cgtggcgcag ccggatgcct ataaagacaa
    14761 agcgcgtaag gcagcgattg ataacgcgat tcatcaggcg caggaactgg cgaacggctt
    14821 tcatcgtaaa ctggggccgg tatatagcgt gcgctaccat gtttccaact atcagcccag
    14881 cccaatggtg cggatgatga aagccgatgc cgcgccggtg tctgcccagg aaacttacga
    14941 gcaggccgct attcagtttg atgatcaggt cgatgtggtc ttccagttag aacctgtgga
    15001 tcaacaaccc gctaaaacac ctgcagcaca ataatacttg tagcccgacg tattcgtcgg
    15061 gctaatgcct ttacccgata ttgcctcctt tttctgtaat tttctgtaaa accatgacgt
    15121 aatcacctat ctttgctcgc ggtttcatca aggataggct gactgaaacg gttaagcgga
    15181 caaggatgcg gggagggggg atggacattt ttatttcgaa aaagatgcgc aatttcattt
    15241 tattagcgca aaccaataat attgcccggg cggcagaaaa aatccatatg acggcttcgc
    15301 catttggtaa aagcattgcc gcgctggaag aacaaattgg ctatacgcta tttacccgca
    15361 aagataataa tatcagcctc aataaagccg gtcaggaact gtatcaaaaa ctgttcccgg
    15421 tttatcaacg actttctgcg attgataatg aaatccataa ctcagggcgt cgttcacgag
    15481 aaattgtgat tggcatagat aatacttatc cgacaattat tttcgatcag ttgattagtc
    15541 tcggagataa gtatgaaggc gtgaccgccc agccagttga gttcagcgaa aacggcgtta
    15601 tcgataatct ttttgaccgt cagttagatt ttattatctc cccacagcat gtatctgcac
    15661 gagttcagga actggaaaac ctgactatca gtgaattacc accgctgcga ttgaactttc
    15721 tcgtttctcg ccgttatgaa gagcgtcaag agcaagagct gcttcaggag ctgccctagc
    15781 tacaaatgcg ctttcaaaac cgggctaact tcgaagcaat gattgatgcg aatatgcgac
    15841 cttgtgggat caatcccacc attatttatc gtccctacag ttttatggcc aaaatcagcg
    15901 ccgtggagcg cggacatttt ctgacggtga tcccgcattt tgcctggcga ctggtgaatc
    15961 cggcaacgct gaaatatttt gatgcgccgc acaggccgat gtatatgcag gaatatcttt
    16021 attcaatcag aaatcatcgt tataccgcca cgatgcttca gcatattgct gaagatcgtg
    16081 acgggacaaa ccattaaccc agcatcgagc cggttgcaat taaattacgg tgtaagtcga
    16141 agacgtggct aagatcgtgg tgaatatgtc cgccgggcgc attttccaga tagcgatgca
    16201 gataatcccg atacatagga tgtgcacaat tatcaatgat agtgcgggcg cgttgaagcg
    16261 gggaaagacc gcgcagatcg gcgatccctt gttcggtgat gatcactttg acgctgtgtt
    16321 cgttgtgatc aacatggctg cacattggca cgacggttga gatcttgcct tctttagcaa
    16381 tcgacggagc cataaagatc gacagatacg cgttgcgttc aaaatcaccg ctgccgccga
    16441 tgccgttcat cagatcgacc ccggctacgt gcgttgagtt ggcatgcccg taaatatcaa
    16501 actccaggcc gacgttcaga gcgatgacgc ccagacgacg gatgatttcc gggttattgg
    16561 aaatctcctg cggacgcaac acaatgcggc tggcaaagta atccatattg tcgtaaatct
    16621 tgcgcaggga atcggccgag attgtcaggc tggaggcgct ggccccgctg attttgccgg
    16681 tttccagtaa atgcaccacc gattcctgta gcacttccga atacatcata aacggaggaa
    16741 tttccgggtt ttcccccaga cgcgccatta ccgcattatt gatattgccc acgccacttt
    16801 gcagcggcag aaattccggc ggaatacgcc catgcgccat ttcctgcaat aagaacgtga
    16861 ccacgttatc ggcaatctgc tggcacatgg gattttgctt atccagcata ttaccggcgt
    16921 cgggcaagtt ggtttccacg acggcgacaa tctttttcgg atcgatttgc acatagcggg
    16981 taccgacgcg atccattgca tggaagatcg acacgctatt gcgccgcggt ggcgcgccag
    17041 gaatcacaat atccgccagt tctgcaacgc gcggatcgtg atagtgattg agttcaatga
    17101 tcactttctt cgctcgcagc agccaggtcg gcgcattacc gatcccgctg gttaaccaga
    17161 ctcgaccatc tggtgccagt gccgatgctt caatgacggc aacatcaatg tcgccgaaga
    17221 aaccgtaatt gaccatttgc gccacttcgc tcaaatgcag gtcaacgaaa ctcaccgcgc
    17281 cctgattgat ctttttacgt aaaccggacg atgtttgata tggcgcacgc caggaaacag
    17341 catcggcgtc agaaagtaca tcgtcagcgg cggcgctgat tgacgccccc gtcagcaggc
    17401 gaatttgata cggctttttg gcctcatgct gttcgttagc tctgcgggca atcgcggtgg
    17461 gtagggcttt cggcgaaccc gctggggtaa agccgctaaa tgccaccatg tcgttatgct
    17521 ggataatttc tgccgcttca tcggcggtca tccttgtcca ctgagtttcc atttttactc
    17581 cttcgttcgc agggattaat gaccaacgaa attaggttta cgtttttcga ggaaagcgtt
    17641 catcccttcc tggtaatctt cgctgtcata aaccgcgcgg cgcatcccct gaatacgttc
    17701 aaattcatcg gagttcatgg tgtgtgcttc gcccagtaca cgcagctctt ctttgataac
    17761 ggcaatggct aacggcgctt tctcagagat gtggtgcgcc atttgtaagg tgaaatcttc
    17821 cagttcttcc acttccacaa catggttgag gatgccgaca gccagcgcgc gctgggcggt
    17881 gatgggcgaa gcggtaaaaa tcagctcttt gacaatgtgg aagcccgcgt cgcgggtcag
    17941 gttgtgaatg ccgaccaggt tatacgggac gccgaggttt acaggcgtca ttgagaaggt
    18001 tgaggtactg gcggcgataa tcagatcgga actcatgatc atttcaaatg cgccacccca
    18061 aacactacct tccaccatcg aaataatcgg tttcgggaat ttttggatca tgcgggtgat
    18121 ttgacgcaat ggatcatcat aggagagcgg atcgcgaccg ccagacggca gttcgtgaat
    18181 atcgtgacct gcggagaaga ctttggatcc actcggcgcg cgcaaaatga tacagcgaat
    18241 ttccggccgg ttgagatcgc ttaacgcctg cataagatca tcaataaaga ctttacttaa
    18301 ggcattaagt tttcggccat agttaaactc aatgaccgcc actttgttga tagtgacaac
    18361 gttaacatac tgataagaca taaaaattcc tttaatcaaa atattgcgtc tggataaatt
    18421 cactgagctg ccgcaggccg gtgcgcggtg agagcgtatt gtttttgacc gctaaaagcg
    18481 tctggcggta atagcgatcg aaatcttcat tcgcgaacag gtgattcagt acttcttctt
    18541 cggtctgctt acgcagccat tccaccgatt gttgttgccg cacttgttgt aaacgaccac
    18601 tggcagttag cgcggttttg aagtcgatga tggcgtgcca gatctcatcg attccacgtt
    18661 tttccagtgc gctacaagtc agaacccgtg gctgccattc gtcgtattta cgtcgcagaa
    18721 tatgcagggc actctcgtac atatgccggg caatggcgac attggtatgg ttatcgccat
    18781 cgtctttgtt gataacgatc agatcagcca cttccatcag ccctttttta atgccctgca
    18841 gatcatcgcc gccaccggca atttgcaacg agataaaaca gtccaccatg cgggcgactt
    18901 ctgtttccga ctgcccgacg ccaaccgttt cgacaatcac tacgtcataa cccgctgctt
    18961 cgcataacag cattaattcc cgcgctcgct gactggcacc gcccagatga ccggaggatg
    19021 gtaccgggcg aataaacgcc gcttcggcac gcgccaggtc attcatgcgg gttttatccc
    19081 cgagaatgct accgccagtg accgggctgc tgggatcgac cgcaataacc gcgaccttta
    19141 atccctctcg aatcaacaac atgccaaagg cctcaagaaa ggtacttttc cccgcgccgg
    19201 gggtgccggt aacgcccagt cgcagggtgt taccgcagta cggcataatg gcatcaagca
    19261 gctgcgtact tagtgcctga tgacgcgggt gacggctttc caccagcgtc atggcctggg
    19321 cgagtgtggc atgctcaccc tgacgtaagc ggcgaatact ttctgccagc gtggcttcat
    19381 taatcatgat gctggcttat cagattcagt acgtcgcgca cactgtcgag cataggtgta
    19441 cctggaccat aaatcgccgc cacgccgcgc tcttgcagga aggcgtaatc ctgcggtgga
    19501 atgacgccac cggcgactac gcagatatct tcgcgtcccc attttttcag cgcttcgacc
    19561 agttccggga tcagcgtttt atgaccggca gccagtgagg atgcgcccac tacgtgaacg
    19621 tcattttcta ctgccaggcg ggcgatctct tcaggtgtag agaacatcgg gcttaaatct
    19681 acgtcgaaac cgagatcgga ataggcgctg gcgatcactt tcgcgccgcg atcgtgtcca
    19741 tccaggccca ttttggcgat cagaatgcgc gggcgacgac cattgtcggc aaggaactgc
    19801 tccgtttgcg caacaatggc atcgaactcg gaggccgatt tctcagattg atgatagctt
    19861 tgcgcaatca cgccggtaac acactggctt ggcaccagat aacggtcgaa agcggcttcc
    19921 agcgcatcgg aaatttcacc cagggtggcg cgaacgcgag cggcattaac agcggcagcc
    19981 agcaggtttt cgttatgctg tgcggcgtga gtcagggcgt tcaacgcggc ggttacggcg
    20041 gcatcatcac gggtggcgcg aatgcgttcc agcgaagcaa tttgctcgtt acgcaccatc
    20101 acgttgtcga tctcaagtac atcggtttcg tcttcgtgat ccagtttgta cttgttgaca
    20161 ccaacgatga cacgcttgcc ctggtcgatc agcgactgtt cgcgcgctga ggcctcttcg
    20221 atcattcgtt ttggcagacc tgcttcgatc gctttcgcca tgccaccggc ttcgtcgatc
    20281 tgttggataa tagctctggc ttgtttgacg atttgatcgg tcagcgactc aatgtaatag
    20341 gatccggcca gtggatcgac ggtgcggcag agttctgatt cttcctggat gatgatctgg
    20401 gtgttgcggg caatgcgtgc tgagaaatcg gtaggcaaac caagcgcttc gtcaaaggcg
    20461 ttggtatgca gtgactgagt accgcccagc gtcgcagcca gcgcttcaat ggtggtgcgg
    20521 ataacgttgt tatacggatc ctgttcagtc aggctccagc ctgaggtctg gcagtgggta
    20581 cgcagcgcca gtgatttcgg gtcctgtgcg ccaaatccac tgaccgcttc gctccataaa
    20641 taacgtgccg cacgcaacat ggcgacgttc ataaacagat ccatgccgat gccgaagaag
    20701 aacgacaggc gaggagcgaa gtcatcaatt ttcagtccgg cagagattgc tgctttgatg
    20761 tactcaatcc catcagcgag cgtaaatgct acctgctgca cgcagttggc acccgcttca
    20821 cccatgtggt aaccgctgat actgatggta ttaaatcgcg gcatgttgcc ggaacaccag
    20881 gcgatgatgt cggcgataat gcgcattgac ggttttggcg ggtaaatata ggtgttgcgg
    20941 cagaggtact ctttgagaat atcgttttga atggtgccgg tcagtttatc aggtgtaaca
    21001 ccttgctctt ccgcggcgat gatataaaac gccagtactg gtagcactgc gccattcatg
    21061 gtcatcgaaa ccgacatttt atccagcggg atctggtcga acaggacttt catatcttcc
    21121 acggtgtcga tagcgacgcc cgttttgccg acgtcacccg ccacgcgtgg gttatcggag
    21181 tcgtagccac ggtgggtggc aaggtcaaac gcaacggaaa gacctttttg cccggcggcc
    21241 aggttacggc gataaaaagc gttggactct tttgctgttg aaaaaccagc atactgacgg
    21301 atggtccacg gttgggcggt atacatagtg gcacgcgggc cacgaacgta gggcggcaaa
    21361 ccaggaaggg tacctgtcac ctccagatta tcgagatcgg cttcggtata cagcggcttg
    21421 atggcgatcc cttccgcggt ttgctgaacc agcgagtcga cagttttctc ccgacggctc
    21481 aattccttgt tggcaagctg ttgccactcc tgctcgttag acattatttg ctccgtaaaa
    21541 cgcaatcaaa atgagggtat ttgcgcttac ggtgagcttt ttacgtgggt ttgtcgccag
    21601 caaaaaaaat gcattcagac gtcgtttttt gccgcctgag atttgtctat tttgtgatgt
    21661 gattcaagga atacaaaaag aagtgaggat aacgcctgat atgtgctatc aggcattatt
    21721 tgatggatta atcctgacga aggactttgt gaccataatc gagcaacgca tcagtgactt
    21781 tacgcatcat gcggctttca ggagcaaagc ggtgccagta gagcatccgg cgttgaaata
    21841 gccctggcgt taagtcaatc agttcaccgc tggctagttc tttctcgatt tgcaggtgcg
    21901 ggatcataca gcaggtggtg ccctggcgag caagttgcac gaacgcttct gaagaattaa
    21961 cgatatggca gggcacgctg cctggaggca gatcgaagtt ttgctgcaaa aaggcctggt
    22021 gcatatcgtc aagatggtca aacgcgacca ctggcgcttt cagtaatgcc gaacgcgtta
    22081 cgccgttagg gaaatatttt tcggcaaagg gttttgagct gacgaacaga tagtcgagcg
    22141 caccaagttt atcgacaaga caactcggca gcgcctgatg ttgaatactc accgcgccga
    22201 ccacttcgcc gcgacgcaga cgttcctgag tgcgggtttc atcttctacc tgcaagttga
    22261 ggcggatagg cgaatcagcc aatacaggag ccagtgccgg aagcaaccac gtcgccagac
    22321 tgtcggcatt gaccgccagt gaaagcagca gcggagtcga accggtttgt tcatcgccca
    22381 gccactcttc ttccagcaac tccacctggc gcagcagtgc cagcagtttt tgcccttgtt
    22441 ccgtcgggcg cggcggtacg gtacgcacca acagcggctg cccgaacata ttttccagtt
    22501 gcttaatgcg ctgtgagacg gctgattgtg taatgcacag cttttgtgcc gcgcgctcaa
    22561 atcctcgttc acgtatcacc gcatccagtg cctgtaatgt tctgtagtcc gggcgtttca
    22621 ttgctgttgt ctgctcctgg taaatgcttt ttcctgcact atgacacaat tttgtgtcag
    22681 gttgcatata ccgcagccgt cggcgggtaa catttgtatc aatggttaaa cgcgcagctt
    22741 aaaagatcag ggatttgcgc tattgcattt cgcttagcaa cttcgcgccc tggttatgtt
    22801 gaatggcgtg gcgttattgc ctctgttcgc accgtggagc ggcataccca gaccgttgta
    22861 tgctttattt gcggggcgaa aggggatgcc cgccattgcg cgatatttta tggatgagtt
    22921 aaccacgcgg cttgccaacg gggtctgaat cgcttttttt gtatataatg cgtgtgaaat
    22981 ttcataccac aggcgaaacg atcatgacgc aggatgaatt gaaaaaagca gtaggatggg
    23041 cggcacttca gtatgttcag cccggcacca ttgttggtgt aggtacaggt tccaccgccg
    23101 cacactttat tgacgcgctc ggtacaatga aaggccagat tgaaggggcc gtttccagtt
    23161 cagatgcttc cactgaaaaa ctgaaaagcc tcggcattca cgtttttgat ctcaacgaag
    23221 tcgacagcct tggcatctac gttgatggcg cagatgaaat caacggccac atgcaaatga
    23281 tcaaaggcgg cggcgcggcg ctgacccgtg aaaaaatcat tgcttcggtt gcagaaaaat
    23341 ttatctgtat tgcagacgct tccaagcagg ttgatattct gggtaaattc ccgctgccag
    23401 tagaagttat cccgatggca cgtagtgcag tggcgcgcca gctggtgaaa ctgggcggtc
    23461 gtccggaata ccgtcagggc gtagtgaccg ataacggcaa cgtgatcctc gacgtccacg
    23521 gcatggaaat ccttgacccg atagcgatgg aaaacgccat aaatgcgatt cctggcgtgg
    23581 tgactgttgg cttgtttgct aaccgtggcg cggacgttgc gctgattggc acacctgacg
    23641 gtgtcaaaac cattgtgaaa tgatctgacg ggggaacctc ccccgttaaa aaaattctct
    23701 tcattaaatt tggtgacatg tgtcacgttt ttacctggat attgtcgatt gctctaaata
    23761 aatcctctaa accagcatat tcaaccaaga aatacctttg cgtgatattt cctcaacatc
    23821 gcgacgcaaa cgttcatatt gccgcaatat tattttttga tatgttgaaa ggcggatgca
    23881 aatccgcaca caacatttca aagacaggat tgggtaaatg gcaaaggtat cgctggagaa
    23941 agacaagatt aagtttctgc tggtagaagg cgtgcaccaa aaggcgctgg aaagccttcg
    24001 tgcagctggt tacaccaaca tcgaatttca caaaggcgcg ctggatgatg aacaattaaa
    24061 agaatccatc cgcgatgccc acttcatcgg cctgcgatcc cgtacccatc tgactgaaga
    24121 cgtgatcaac gccgcagaaa agctggtcgc tattggctgt ttctgtatag gaacaaacca
    24181 ggttgatctg gatgcggcgg caaagcgcgg gatcccggta tttaacgcac cgttctcaaa
    24241 tacgcgctct gttgcggagc tggtgattgg cgaactgctg ctgctattgc gcggcgtgcc
    24301 ggaagccaat gctaaagcgc accgtggcgt gtggaacaaa ctggcggcgg gttcttttga
    24361 agcgcgcggc aaaaagctgg gtatcatcgg ctacggtcat attggtacgc aattgggcat
    24421 tctggctgaa tcgctgggaa tgtatgttta cttttatgat attgaaaaca aactgccgct
    24481 gggcaacgcc actcaggtac agcatctttc tgacctgctg aatatgagcg atgtggtgag
    24541 tctgcatgta ccagagaatc cgtccaccaa aaatatgatg ggcgcgaaag agatttcgct
    24601 aatgaagccc ggctcgctgc tgattaatgc ctcgcgcggt actgtggtgg atattccggc
    24661 gctgtgtgat gcgctggcga gcaaacatct ggcgggggcg gcaatcgacg tattcccgac
    24721 ggaaccggcg accaatagcg atccatttac ctctccactg tgtgaattcg acaacgtcct
    24781 tctgacgcca cacattggcg gttcgactca ggaagcgcag gagaatatcg gcctggaagt
    24841 tgcgggtaaa ttgatcaagt attctgacaa tggctcaacg ctctctgcgg tgaacttccc
    24901 ggaagtctcg ctgccactgc atggtggtcg tcgtctgatg cacatccacg aaaaccgtcc
    24961 gggcgtgcta actgcgctga acaaaatctt cgccgagcag ggcgtcaaca tcgccgcgca
    25021 atatctacaa acttccgccc agatgggtta tgtagttatt gatattgaag ctgacgaaga
    25081 cgttgccgaa aaagcgctgc aggcaatgaa agctattccg ggtaccattc gcgcccgtct
    25141 gctgtactaa ttccccttct ctgaaaatta acgggcaggt cactgacttg cccgtttttt
    25201 tatcccttct ccacaccgtt gccgcgttgc gcattctact ttgcgagtct gctccagaaa
    25261 ataaatcaaa gtcagatcaa tgcgttgtgt ttatttaatg gggatttacg atggcgggac
    25321 agcatggggc tgtaacggtg aagccctcac cgaagcgagg gcttaaagga gaagggttat
    25381 gatgcgactt gtcatcatac tgattgtact gttactgata agtttcggag cttattaaca
    25441 gtcagtctca ggggaggagt catcctccct tacccttact cactaaatta ggtcaaagaa
    25501 tcaacgatgt caatcagggc gatgcggggt gcatcgccct gaaccgaact ttttgatagc
    25561 cacttcatcg cccacgttta ctgccgtaat taatccctta ttcctgcaat gtatgtctgc
    25621 cttacatgac gttttacgag tgcgctcgaa gaaaagattt aaaggtagat caatgcgttg
    25681 tggttattta aagagagttt acgatggcag ggcagcatgg ggctgtaacg gtaaagccct
    25741 caccgaagcg agggcttgaa ggagaagggt tatgatgcga cttgtcatca tactgattgt
    25801 actgttactg ataagtttgc cagcttatta acagtcagtc tcaggggagg agtcatcctc
    25861 ccttaccctt actcactaaa ttaggttaaa gaatcaacga tgtcaatcag ggcgatgcag
    25921 gtgtatcgcc cttaccactc ccagactttc gacggtgtaa ccaccgcagg aagagggata
    25981 tcccactctt caacggggag tttttccacc aactgacaat catgcgcata acccaccggt
    26041 tgcgttttat agtgctgcca gttttgtaag gtccgatcat aaaaaccacc gcccattccc
    26101 aggcgctgac cgtactcatc aaaggcgacc agcggtgtga tcagcacgtc taatcgggaa
    26161 agggggagca cgtcacgcac atccagtttt ggctcatgga ttttcaacct gttcatcacc
    26221 agttcgcttt gcggatggta attcaggaac agcaaattac cggcactaaa gggatgcaaa
    26281 actggaagat atacgcgctt accggcgcgc cagagttgtt ctataagcgg ctgggtgtcg
    26341 agttcaccat caaaagagag gaatacagcg accgtatgcg ccatcaccac cgggggataa
    26401 gtcatcatcc gggtagcggc ttgttgaccc atttcctgct gttgttccgg cgttaacgca
    26461 cgacgacgtt gccgaatcat tttgcggatt tcttgtcggg ataatgtcag tgggagttct
    26521 ggtagttgcg tcatggctgg tgccagataa gaagggaatc tccgagatgc cgccgcaggc
    26581 tgtaaccctt gaacccttgg ttcaaggtga atgtgtcgtc gcagttttaa ggcttctcgg
    26641 acggaccgag catgctcacc aaccgcggag cgccacattc ttgtggtatg aaatatcggc
    26701 tcaggggact ggcccgcttg cgaacatctc agagaaattt tgtcttcacg gttactctac
    26761 cacagtaaac cgaaaagtgt cattcaaagt tttggttagt tttttcggtg atgcgacctt
    26821 gttcaagtaa cgcttgttct atggtctgct gcagcatccg aatacgctgt tccatacttg
    26881 ccgcgtagtc acgagtcttc gctttttctt gcgctaactc atagctgata ttcaatgcgg
    26941 caatgaagac caactgttca gtatttgtga ctctagtgcg ttctttcaga tcttgcaacc
    27001 gttggttcag atcgtccgct gcctgattca acgcatccct ttggtcaggc gggcagttca
    27061 cacgcagtga acggccaaaa atttggatat cgacgggttg tgcagacatg ccaccttcct
    27121 gctgattgac tgcgctgcct tcgtctttag acccatggtc tgcgaagggg cgacactata
    27181 gctaccctga tgagaagaga caagcccttt tctggtccac cagggcccaa agtggtagca
    27241 tatcatgaat attcctccct ttgacgacga atgcttatgt ctatacagaa cgaaatgcct
    27301 ggttacaacg aaatgaacca gtatctgaac caacaaggga cgggtctgac ccctgctgag
    27361 atgcatggtt taatcagtgg gatgatatgt ggcggtaacg atgacagctc atggctaccg
    27421 ctacttcacg acctgacgaa cgaaggcatg gctttcggtc atgagctggc acaggcattg
    27481 cgtaaaatgc actctgccac cagcgatgcc ctgcaggatg acggcttcct ttttcagctt
    27541 tatctgcctg atggcgatga tgtcagcgtt ttcgatcggg ctgatgcatt ggcaggttgg
    27601 gtcaatcact tcctgcttgg tcttggtgtt acgcaaccga agctggataa agtgaccggc
    27661 gaaaccggtg aagctatcga cgatctgcgt aacattgcgc aactgggtta cgacgaagac
    27721 gaagatcagg aagagcttga aatgtcgctt gaagagatca tcgaatacgt tcgtgttgcc
    27781 gcgctgttat gccacgacac ctttactcat ccgcaaccga ccgcgccaga agtacaaaaa
    27841 ccgactctac actaaaaaca aaaaacgtaa ggagagtgtt atgagtgaga tatcccggca
    27901 agagtttcag cgtcgccgtc aggccctggt ggagcaaatg caacccggca gcgccgcgct
    27961 gatttttgct gcaccagaag taacacgtag cgccgacagc gaatacccct atcgtcagaa
    28021 cagtgacttc tggtacttca ccggctttaa cgaaccggaa gcggtgttgg tgctgattaa
    28081 aagcgatgac actcataacc acagcgttct gtttaaccgc gttcgcgacc tgacggcgga
    28141 gatctggttt ggccgtcgct taggccagga tgccgcgcca gagaaactgg gcgttgaccg
    28201 cgcactggca ttcagcgaaa ttaatcagca actttatcaa ctacttaatg gtctggatgt
    28261 ggtttaccat gctcagggcg aatatgcata tgctgatgaa atcgtgaaca gtgcgctgga
    28321 aaaactgcgt aaaggttcgc ggcaaaatct caccgcaccg gcaacgatga tcgactggcg
    28381 tcctgttgtt catgaaatgc gcctgttcaa atcgccagaa gagattgccg tactccgccg
    28441 cgcgggagaa atcaccgcca tggcacatac acgggcgatg gaaaaatgcc gtccgggaat
    28501 gttcgagtac catctggaag gcgaaattca ccacgaattt aaccgccacg gtgcgcgcta
    28561 tccgtcctat aacaccattg taggcagcgg tgaaaacggc tgcattttac actacaccga
    28621 aaacgagtgt gaaatgcgcg acggcgacct ggtgttgatt gacgcaggct gcgaatacaa
    28681 aggttacgca ggcgatatca cccgtacctt cccggtcaac ggcaaattca cccaggccca
    28741 gcgcgaaatc tacgacattg tgctggagtc tcttgaaacc agcctgcgcc tgtatcgtcc
    28801 gggaacctcc attctggaag tcaccggtga agtggtgcgc attatggtta gcggcctggt
    28861 aaaactcggc atcctgaaag gtgatattga tgaactgatc gctcagaacg cccatcgtcc
    28921 tttctttatg catggcctta gccactggtt aggactggat gtccatgacg ttggcgttta
    28981 tggtcaggat cgctcgcgca ttctggaacc ggggatggta ctgaccgtag agcctggtct
    29041 gtatatcgcg ccggatgcgg atgtgccaga acaatatcgc ggtatcggca ttcgtattga
    29101 agacgacatt gtgattaccg aaaccggcaa cgaaaacctc accgccagcg tggtgaaaaa
    29161 gccggaagaa atcgaagcgt tgatggctgc tgcgagaaag caatgagcgt aatcatcgtc
    29221 ggtggcggca tggcgggcgc gacgctggcg ctggctattt cccggttaag tcacggggcg
    29281 ctgccggtac atttgattga agcgactgcg ccagagtcac atgctcatcc gggctttgat
    29341 ggacgagcga tagcgctggc ggcgggtacc tgtcagcaac tggcgcgcat cggcgtttgg
    29401 caatctctgg cgcattgcgc aactgccatc accaccgtgc atgtcagcga tcgaggtcac
    29461 gctggatttg tcaccctcgc cgcagaagat taccaactgg cggcgctggg acaggttgtc
    29521 gaattgcaca atgtcgggca acggctgttt gcattgctgc gtaaagcacc tggcgtaacg
    29581 ctgcattgcc ctgatcgcgt ggctaacgtt gcccgtactc agagtcacgt tgaagtgacg
    29641 ctggagggtg gcgagacgct gacgggccgc gtgctggtag ccgctgatgg cacccattca
    29701 gcgttagcca ctgtgtgcgg cgttgactgg cagcaggagc cttacgaaca actggctgtg
    29761 attgccaacg ttgcgacttc cgttgcgcat gaagggcgcg cttttgaacg ctttacgcaa
    29821 catggcccgc tggcgatgtt gccgatgtct gacggacgct gttcgctggt ctggtgtcat
    29881 ccactggaac ggcgcgaaga ggtgctgtcg tggagcgacg agaagttttg ccgtgaactc
    29941 cagtcggcct ttggctggcg acttgggaaa attacccacg ctggtaaacg cagtgcttat
    30001 ccgctggcgt taacccacgc cgccagatct attacccatc gtaccgtgct ggtgggcaat
    30061 gcggcgcaaa ccctgcaccc gattgccggg caagggttca acctcggtat gcgagatgtc
    30121 atgagtcttg cggaaaccct gactcaggcg caggagcgcg gagaagacat gggtgattac
    30181 ggcgtattgt gtcgttatca gcagcgtcga cagagcgatc gcgaagcaac cattggcgtc
    30241 acggacagcc ttgtacatct ttttgccaac cgttggacgc cactggttgt cgggcgcaac
    30301 atcgggctga tgacgatgga attattcacc ccggcacgcg atgtgctggc gcagcgcacc
    30361 ctcggttggg tggcgcgttg aaggcggtta ttaaggaata aacaatgcaa agtgttgatg
    30421 tagccattgt tggcggcggc atggtggggc tggcggttgc ctgcggctta cagggtagcg
    30481 gcttacgcgt tgccgtactg gagcagcgcg taccggaacc tctggcggcg gatgcaccac
    30541 cacaactgcg cgtttcggct atcaatgccg ccagcgaaaa attactcacc cgtcttggcg
    30601 tctggcagga cattctctct cgccgagcca gctgttatca cggtatggaa gtgtgggaca
    30661 aagacagttt tggtcacatc tcgtttgacg atcaaagcat gggctatagc catcttgggc
    30721 acatcgttga aaactcagtg attcactacg cgctgtggaa caaagcgcag cagtcgtcag
    30781 atatcactct tttggccccc gcagaattac agcaggtcgc ctggggagaa aatgaaacct
    30841 tcctgacgct gaaagatggc agtatgttaa cggcgcgtct ggtgattggc gcggacggcg
    30901 ctaattcctg gttgcgcaac aaagccgata ttccgctgac tttctgggat tatcagcatc
    30961 acgcgctggt agcgaccata cgcacggaag aaccgcatga tgcggtggcg cggcaggttt
    31021 tccatggcga aggcattctg gcctttttac cgcttagcga tccgcatctt tgctcgattg
    31081 tctggtcact gtcgccagag gaagcgcagc ggatgcagca ggcaagtgaa gacgaattta
    31141 atcgcgcgtt aaatatcgct tttgataatc gcctgggctt atgcaaggtt gagagcgcgc
    31201 gtcaggtgtt cccactgacg gggcgttatg cgcgccagtt tgccgcgcac cgtctggcgc
    31261 tggtgggcga cgccgcgcat accattcacc cgctggcggg gcagggggta aatcttggct
    31321 ttatggatgc tgcagagctg attgctgaac tgaaacggtt gcatcgtcag ggtaaagaca
    31381 tcgggcagta catttatctg cgtcgctatg agcgtagccg caagcacagt gcggcgctga
    31441 tgctggctgg tatgcaggga ttccgcgatc tgttttctgg taccaatccg gtgaaaaaac
    31501 tgctgcgtga tattggtctg aaactggccg acacgcttcc tggcgttaaa ccacaactta
    31561 tccgccaggc aatgggatta aacgatttgc ctgaatggct gcgttaaaaa tttctcttct
    31621 gctgtttatt tgatacccat cacactttca tctcccggtt ttttcgccgg gagattttcc
    31681 tcatttgaaa taaactaatt tcacctccgt tttcgcatta tattttctaa tgtcacgaat
    31741 ttttgattta gtgttttttg acattttttc agctcttaat attgccttat tcaaattaac
    31801 tttctcatca catcatcttt atatagaaac ttgtgtattt tttggttttt tattctgttg
    31861 cgattttggc gttttttaac cataagctaa tgtgatgatc aattttacct tatggttaac
    31921 agtctgtttc ggtggtaagt tcaggcaaaa gagaacgatt gcgttgggga ccgggagtgg
    31981 ctccgatgct gggtttcgtg gtgataattt caccatgaaa aagttgtcag ccccgcttat
    32041 tcaatgagga caagatggca caacagactc ctttgtacga acaacacacg ctttgcggcg
    32101 ctcgcatggt ggatttccac ggctggatga tgccgctgca ttacggttcg caaatcgacg
    32161 aacatcatga ggtacgtacc gatgccggaa tgtttgatgt gtcacatatg accatcgtcg
    32221 atcttcgcgg cagccgcacc cgggagtttc tgcgttatct gctggcgaac gatgtggcga
    32281 agctcaccaa aagcggcaaa gccctttact cggggatgtt gaatgcctct ggcggtgtga
    32341 tagatgacct catcgtctac tactttactg aagatttctt ccgcctcgtt gttaactccg
    32401 ccacccgcga aaaagacctc tcctggatta cccaacacgc tgaacctttc ggcatcgaaa
    32461 ttaccgttcg tgatgacctt tccatgatcg ccgtacaagg gccgaatgcg caggcaaaag
    32521 ctgccacact gtttaatgat gctcagcgtc aggcggtgga agggatgaaa ccgttctttg
    32581 gcgtgcaggc gggcgatctg tttattgcca ccactggtta taccggtgaa gcgggctatg
    32641 aaattgcgct gcccaatgaa aaagcggccg atttctggcg tgcgctggtg gaagggggcg
    32701 ttaagccatg cggtctgggc gcgcgtgaca cgctgcgtct ggaagcgggc atgaatcttt
    32761 atggtcagga gatggacgaa accatctctc cgttagccgc caacatgggc tggactatcg
    32821 cctgggaacc ggcagatcgt gacttcatcg gtcgtgaagc cctggaagtg cagcgtgagc
    32881 atggcacaga aaaactggtt ggtctggtga tgaccgaaaa aggcgtgctg cgtaatgaac
    32941 tgccggtacg ttttaccgat gcgcagggca accagcatga aggcattatc accagcggga
    33001 ctttctcccc gacgctgggt tacagcattg cgctggcacg cgtgccgaaa ggtattggcg
    33061 aaacggcgat tgtgcaaatt cgcaaccgtg aaatgccggt taaagtgaca aaacctgttt
    33121 ttgtgcgtaa cggcaaagcc gtcgcgtgat ttactttttt ggagattgat tgatgagcaa
    33181 cgtaccagca gaactgaaat acagcaaaga acacgaatgg ctgcgtaaag aagccgacgg
    33241 cacttacacc gttggtatta ccgaacatgc tcaggagctg ttaggcgata tggtgtttgt
    33301 tgacctgccg gaagtgggcg caacggttag cgcgggcgat gactgcgcgg ttgccgaatc
    33361 agtaaaagcg gcgtcagaca tttatgcgcc agtaagcggt gaaatcgtgg cggtaaacga
    33421 cgcactgagc gactccccgg aactggtgaa cagcgaaccg tatgcaggtg gctggatctt
    33481 taaaatcaaa gccagcgatg aaagcgaact ggaatcactg ctggatgcga ccgcatacga
    33541 agcattgtta gaagacgagt aacggcttta ttcctcttct gcgggagagg atcagggtga
    33601 ggaaatttac acctcaccct cactctcttc gcaaggagag aggttcacaa ttcactgcac
    33661 gtttcaggaa ccatcgctca tgacacagac gttaagccag cttgaaaata gcggcgcttt
    33721 tattgaacgc catatcggac cggacgccgc gcaacagcaa gaaatgctga atgccgttgg
    33781 cgcacaatcg ttaaacgcgc tgaccggcca gattgtaccg aaagatattc agcttgcgac
    33841 cccgccgcag gttggcgcac cggcgaccga atacgccgcg ctggcggaac tcaaggctat
    33901 tgccagtcgc aataaacgct tcacgtctta catcggcatg ggttacaccg ccgtgcagtt
    33961 accgccggtt atcctgcgta acatgctgga aaatccgggc tggtataccg cgtatactcc
    34021 gtatcaacct gaagtctcgc agggccgcct tgaggcactg ctcaatttcc agcaggtaac
    34081 gctggattta accgggctgg atatggcctc tgcttctctt cttgacgaag ccaccgctgc
    34141 cgccgaagca atggcgatgg cgaaacgcgt cagcaaactg aaaaatgcca accgcttctt
    34201 cgtggcttcc gatgtgcatc cgcaaacgct ggatgtggtc cgtactcgtg ccgaaacctt
    34261 tggttttgaa gtgattgtcg atgatgcgca aaaagtgctc gaccatcagg acatcttcgg
    34321 cgtgctgtta cagcaggttg gcactaccgg tgaaattcac gactacactg cgcttatcag
    34381 cgaactgaaa tcacgcaaaa ttgtggtcag cgttgccgct gatattatgg cgctggtgct
    34441 gttaactgcg ccgggtaaac agggcgcgga tattgttttt ggttcggcgc aacgctttgg
    34501 cgtaccgatg ggttacggtg gcccacacgc ggcattcttt gcggcgaaag acgaatacaa
    34561 acgctcaatg ccaggccgta ttatcggtgt atcgaaagat gcagctggca ataccgcgct
    34621 gcgcatggcg atgcagactc gcgagcaaca tattcgtcgt gagaaagcga actccaacat
    34681 ttgtacttct caggtgctgc tggcaaacat cgccagcctg tatgccgttt atcacggccc
    34741 gattggcctg aaacgtatcg ctaaccgcat tcaccgtctg accgatatcc tggcggcagg
    34801 cctgcaacaa aaaggtctga agctgcgcca tgcgcactat ttcgacactc tgtgtgtgga
    34861 agtggccgac aaggcgggcg tactggcgcg tgccgaagcg gctgaaatca acctgcgtag
    34921 cgatatcctg aacgcggttg ggatcaccct tgatgaaacc accacgcgcg aaaacgtgat
    34981 gcagcttttc agcgtactac tgggggataa ccacgggctg gacatcgaca cgctggacaa
    35041 agacgtggct cacgacagcc gctctatcca gcctgcgatg ctgcgcgacg acgaaatcct
    35101 cacccatccg gtgtttaatc gctaccacag cgaaaccgaa atgatgcgct atatgcactc
    35161 gctggagcgt aaagatctgg cgctgaatca ggcgatgatc ccgctgggtt cctgcaccat
    35221 gaaactgaac gccgccgccg agatgatccc aatcacctgg ccggaatttg ccgaactgca
    35281 cccgttctgc ccgccggagc aggccgaagg ttatcagcag atgatcgcgc agctggctga
    35341 ctggctggtg aaactgaccg gttacgacgc cgtttgtatg cagccgaact ctggcgcaca
    35401 gggcgaatac gcgggcctgc tggcgattcg tcattatcat gaaagccgca acgaagggca
    35461 tcgcgatatc tgcctgatcc cggcttctgc gcacggcact aaccccgctt ctgcacatat
    35521 ggcaggaatg caggtggtgg ttgtggcgtg tgataaaaac ggaaatatcg atctgactga
    35581 tctgcgcgcg aaagccgaac aggcaggtga taatctctcc tgcatcatgg tgacctatcc
    35641 ttccacccac ggtgtgtatg aagaaacgat ccgtgaagtg tgtgaagtcg tgcatcagtt
    35701 cggcggtcag gtttaccttg atggcgcgaa catgaacgcc caggttggca tcacctcgcc
    35761 gggctttatt ggcgcagacg tttcacacct taacctgcat aaaactttct gcattccgca
    35821 cggcggtggt ggtccgggta tgggaccgat cggcgtgaaa gcgcatctgg ctccgtttgt
    35881 accgggtcat agcgtggtgc aaatcgaagg catgttaacc cgtcagggcg cggtttctgc
    35941 ggcaccgttc ggtagcgcct ctatcctgcc aatcagctgg atgtacatcc gcatgatggg
    36001 cgcagaaggg ctgaaaaaag caagccaggt ggcaatcctc aacgccaact atattgccag
    36061 ccgcctgcag gatgccttcc cggtgctgta taccggtcgt gacggtcgcg tggcgcacga
    36121 atgtattctc gatattcgtc cgttgaaaga agaaaccggc atcagcgagc tggatattgc
    36181 caagcgcctg atcgactacg gtttccacgc gccgacgatg tcgttcccgg tggcaggtac
    36241 gctgatggtt gaaccgactg aatctgaaag caaagtggaa ctggatcgct ttatcgacgc
    36301 gatgctggct atccgcgcag aaattgacca ggtgaaagcc ggtgtctggc cgctggaaga
    36361 taacccgctg gtgaacgcgc cgcacattca gagtgaactg gtcgccgagt gggcgcatcc
    36421 gtacagccgt gaagttgcgg tattcccggc aggcgtggca gacaaatact ggccgacagt
    36481 gaaacgtttg gatgatgttt acggcgaccg taacctgttc tgctcctgcg taccgattag
    36541 cgaataccag taattcactg attcgactat cttctaaagg cgcttcggcg cctttttaga
    36601 ttgttaataa agtgcgcttt gtgtgtttcg gctgaggcgt aatataaaat attgcgagct
    36661 caatatatta aaagagtgcc aggcctgatg agcgtagcgc atcaggcaca tcggctaatt
    36721 acagattctc gccgttgctg gcaatcactt ctttgtacca gttaaagctc ttcttacgtg
    36781 aacgcgacat atcgccagta ccgtcgtcat gtttattcac atagataaag ccgtagcgtt
    36841 tgctgtactg cccggtagtg aacgacacgc agtcgatgca gccccacggt gtgtagccca
    36901 tcagatccac gccatcgtaa gttactgctt ttttcatctc ttcaatatgg gcgcgcaggt
    36961 agtcaatgcg gtagtcgtcg ttgatgctgc catcttcttc cactttgtcg taagcgccaa
    37021 aaccgttttc gacaataaac agcggcttct ggtaacgctc atacagttcg caaagtgcgt
    37081 aacgcagacc caccggatca atctgccagc cccagtcaga tgctttaaca tacgggtttg
    37141 gtacgctgcc ttcaaaacca gagatcgcat cgccggtgcc gccttcggcc ttcactgcgt
    37201 tggtcatgta atagctgaaa ccaagataat cgcaggtgcc ttcgcgcagt acatcgagat
    37261 cgctatcttc cattttgatg ttaaatccgc gacgctccca ctcgttcaac acatacgacg
    37321 ggtaatagcc gcgtagctgc acatcggtaa agacgtagcg ttcgcgcatc gactcctgag
    37381 cgaacatcac atcgtccggg ttacatgagt agggatagag cggcaccatc gccagcatac
    37441 agccgacttt catctccagg ttaatgcgac gcgcggcttt cactgccagg gcgctggcga
    37501 caaactggtg atgcagcacc tgatacatcg tctcttccgg gttttcatgc tcggtataca
    37561 ccacgccgga gcagcagtaa ccgaacagcg gtgcacgcca gttacgctgg ttgttaattt
    37621 cgttgaaggt catccagtat ttgactttgt gcttatagcg ttcaaatacc acttccgcga
    37681 aacgtacaaa gaaatcaacc actttacggt tggtccagct accgtattgc tgcaccagat
    37741 gcagcggcat ttcaaagtgg gagagggtga tcaccggttc gatgttgtat ttcagcagtt
    37801 catcgaacag agagtcgtag aacttcagcc cttcttcgtt tggctgagct tcatcgcctt
    37861 ttggaaaaat gcgcgtccag gcaatggatg tacgaaaaca tttgaagccc atttcggcaa
    37921 atagcttgat gtcttcctta tagtgaccat aaaaatcaac ggcttcatgg tttggatagt
    37981 atttgcctgg caagacttct ttggttattt cgcgcggcac gccgtgtgcg ccaccggtca
    38041 gaacgtcaca aatgctcggc ccttttccgc ctttgttcca gccgccttcg acctgatgag
    38101 cggcaactgc gccgccccat aagaaatctt tcggtaaggt gagttttttc actatcatag
    38161 gctccagatg agtcaattct tacaggaaat agtaccatgc agccaaacga catcactttt
    38221 tttcaacgtt tccaggatga cattctggct gggcgtaaaa ccatcaccat ccgcgacgag
    38281 tctgaatcgc acttcaaaac gggtgatgtg cttcgtgtcg gacgttttga agatgacggt
    38341 tatttttgca cgattgaagt caccgcaacc tcaaccgtaa cgctggatac gctgacggaa
    38401 aaacatgcag agcaggaaaa tatgaccctg actgaactga aaaaggtcat tgccgacatc
    38461 tatcccggcc agacacagtt ttatgtgatt gaatttaaat gcctttaaat tgaactcacg
    38521 cctgttagtt gaaatttcgc atttaatttc atgattttta ggagattagt ctcaaatcag
    38581 aatttatttt agctaacagg tgttcactgg aactattctc agttacgctg gagaggtata
    38641 catgtgcgtg tacgtttctc cggagtaagt tatggttcag aagcccctca ttaagcaggg
    38701 atattcgctg gcagaggaaa ttgccaacag cgtcagtcac ggcattgggt tggtgtttgg
    38761 tatcgttggg ctggtgttgc tactggttca ggcggtggat ctcaatgcca gcgccacagc
    38821 gataaccagc tacagcctct atggcggcag tatgatcctg ctgttcctcg cttcgacgct
    38881 ctatcacgcc attcctcatc aacgggcaaa aatgtggctg aagaaatttg accattgcgc
    38941 tatttacctg ttgattgccg gaacctacac gccgtttttg ctggtggggc tggattctcc
    39001 gttagcgcgc gggttgatga ttgttatctg gagcctggca ttgctgggga ttctgttcaa
    39061 actgaccatc gcgcaccgat tcaaaatttt atctctggtg acttatctgg cgatgggctg
    39121 gctgtcgctg gtggtaattt atgaaatggc agttaagctc gcggcgggca gcgttacctt
    39181 actggcggta ggcggcgtgg tttattcgct cggggtgatt ttctacgtct gcaaacgcat
    39241 tccatacaac catgccatct ggcacggctt cgtgctcggc ggtagtgtgt gccactttct
    39301 ggcgatctat ttgtatattg ggcaggcgta atgggtatgc ctttgtaggc ctgataagac
    39361 gcgccagcgt cgcatcaggc gataaatgcc tgatgcgaca cagagattta ctcttcgagc
    39421 gaatacggca gtggctcgat acacaatgta ttcgcatcgt cgcgcacgcg gaagatgctg
    39481 tccggttcca tatcgttatt catcactacc tgcaccacga cctgaccatc ttccagtttt
    39541 accgcagcca gcaccgtacc agtacgacgc cagttctcgc ccattttcag ctctaagtct
    39601 tcaccagctt ccggcagtcg gctggcacta cctgtcagca accagagcgc gcgtttgttg
    39661 gcaccacgga attttgctcg cgccaccatc tcttgtccgg tatagcagcc tttcttaaag
    39721 ctgataccgc ccagcgcctg gaggttggtc gcctgtggga taaactgccc gctgttcgcg
    39781 gcatcaatca ccgggaaacc cgcttcaatg tttaatgcca gccactgttg gctattgttc
    39841 agttccgctt caccgcgcag tttatcggtc agcatattgg cagtagcttc atcggttacg
    39901 atcaggaaac gttctgccgg gtgttcaaac catagcaaag tggtcgcgcc ttctttaact
    39961 acctgttttt ctttcgaagg cagttcacta aagatatttg ccagcgcggc gcgcgcctga
    40021 aaaccggcaa caccaagcag cacacgctcg tcgtctggcg cgatggtcac tttagagaac
    40081 accgcatatt ttttcagttc agccagctgc ggttcacgca cgctgcgccg ttcaatccat
    40141 gcaaagccat cgccgtcgcg gaacagacgt aaattgctcc acattttacc tttggcgtcg
    40201 caatgggcgg cgagcaggtg ctgatcatct gtcatctggc tgacatctgc tgtcacctgg
    40261 ccctgcaaat atttttcgct gtccgcgcca gtaatggtgg caagcgccca gtcatcaagc
    40321 gtcatcagcg tcagtggcaa acgggcagaa gccgtaggct gacggggagg aaaaggtgta
    40381 aaagccataa tagagtcctg agttgcttaa cggcgtatta ataactaatg gtaaaagagc
    40441 tatgaggcaa tgcaagtgct ttcactacgc gatctgtgcc ttctgcgagg cgacttccca
    40501 aaaaatgaag cccttaaatt gttgtgaatt aatttcgcga tcgcgattgt gtttactgat
    40561 attcccgtaa aagaagtcga aaaacacgtt acaatagccg ggtactggat tttcgtgaga
    40621 caggaagaag atggacatta acaacaaagc ccgcattcat tgggcatgcc gccgtggtat
    40681 gcgcgaactc gatatttcaa tcatgccgtt tttcgaacat gagtatgaca gcttaagcga
    40741 tgacgaaaaa cgcatcttta ttcgtctgct ggaatgtgac gatcccgatc tgtttaactg
    40801 gctgatgaat cacggtaaac cggccgatgc agaactggaa atgatggtcc gactcatcca
    40861 gacacggaac cgggaacgtg gtcctgtggc aatctgattt gcgcgtctcc tggcgcgcac
    40921 agtggctttc cttgctgatt catgggctgg ttgccgctgt tattttactc atgccctggc
    40981 cgctcagtta caccccgtta tggatggtgt tactttcgct ggtggtgttt gattgcgttc
    41041 gcagccagcg gcgtattaat gctcgccagg gggaaattcg cttgttgatg gacgggcgtt
    41101 tgcgttggca agggcaggag tggagcatcg tcaaagcgcc gtggatgatt aagagcggca
    41161 tgatgctgcg tttacgttct gatagcggta aacggcaaca tttatggctg gcagccgaca
    41221 gcatggacga agccgaatgg cgggatttac ggcggatttt gttgcaacaa gagacgcaaa
    41281 gataaagatg acggcaaagc atatgcgcta agccgtcagt gatatcaggc gtaatgctct
    41341 gccatttcgt tgaggatttg ctcgcaccag ctctgaatac gctcgtcgct aaggtcatac
    41401 tggttagttt cgtccagcgc cagccccacg aacagttgcc cgtcagcaat caccggtttc
    41461 gggctggtaa attcataccc ttccgttggc cagtagccga cgaacttcac gcctttggtc
    41521 gagagtttgt catgcagcat accgagcgca tcgaggaacc actcgccgta tcccagttga
    41581 tcgccaagcc catacagcgc aacaatttta ccttcgaggt tcaggtcgtc gagctgatcc
    41641 cagacggctt cccagtcttc ctggatttcg ccaaaatccc aggtcgggat acccagaatg
    41701 agcacatcgt actgctccat taatttcggg gagtcgtcct tgaggttatg taaggtcacc
    41761 agttctgggc cgataatatc gcggattttt tctgccgcca tttcggtgta acaggtgctg
    41821 gaaccgtaaa aaagacccat attcataacg taagcttctc gattctgctg tggcttttgc
    41881 ggctagtgta tcagaatcgc tttagtcacg gcacaataag gcataatgga tcaaatgagt
    41941 gaccataagg ggcgcaagtg aaacaggatc tggcacgcat cgagcagttt cttgatgctc
    42001 tgtggctgga aaaaaatctg gctgaaaata cgttgaacgc ttaccgtcgc gatctgtcaa
    42061 tgatggtgga gtggttgcat caccgcgggt tgacgctggc gacggcgcaa agtgacgatt
    42121 tacaggcatt actggcagaa cggctggagg gcgggtataa agccaccagc tcagcgcgtt
    42181 tgctgagtgc agtgcgccga ttgttccagt atctttatcg cgaaaagttt cgtgaagatg
    42241 atcccagtgc gcatctcgct tcaccgaaat tgccccagcg tttgccaaaa gatttaagcg
    42301 aagcgcaggt cgaacgttta ttacaggcac cattaattga tcagccactg gagctacgcg
    42361 ataaagctat gcttgaagtg ttgtatgcta ccgggctgcg tgtctctgaa ctggtcgggc
    42421 tgacaatgag tgatatcagc ctgcgtcagg gcgtagtgcg ggtcattggt aaaggcaaca
    42481 aagagcgcct ggtgccgtta ggtgaagagg cggtttactg gctggaaacc tatctggaac
    42541 atgggcgtcc gtggctactg aatggtgtgt caattgacgt gttgtttccc agccagcgtg
    42601 cgcagcagat gacgcgacag accttctggc accgcattaa acattatgcc gtgctggcgg
    42661 gtatcgacag cgaaaagctg tcaccgcatg tgttgcgtca cgcttttgcc actcacttat
    42721 taaatcatgg tgcggattta cgcgtggtgc agatgctact gggccacagc gatctctcca
    42781 ccacgcaaat ttatacgcat gtcgctaccg agcgtctgcg gcaacttcat caacagcatc
    42841 acccgcgggc gtgatgtctg aaaagaacgg gaagatttat gaagaaaggt tttatgttgt
    42901 ttactttgtt agcggcgttt tcaggcttcg ctcaggctga tgacgcggca attcaacaaa
    42961 cattagccaa aatgggcatc aaaagcagcg atattcagcc cgcgcccgta gctggcatga
    43021 agactgtgct gactaacagc ggtgtgttgt acatcaccga tgatggtaaa catatcattc
    43081 aggggccaat gtatgacgtt agcggcacgg ctccggtcaa tgtcaccaat aagatgctgt
    43141 taaagcagtt gaatgcgctt gaaaaagaga tgatcgttta taaagcaccg caggaaaaac
    43201 acgtcatcac cgtgtttact gatattacct gtggttactg ccacaaactg catgaacaaa
    43261 tggcagacta taacgcgctg gggatcaccg tgcgttatct tgctttccca cgccaggggc
    43321 tggacagcga tgctgagaaa gaaatgaaag ctatctggtg tgcgaaagat aaaaacaaag
    43381 cgtttgatga tgtgatggca ggtaaaagcg tcgcaccagc cagctgcgac gtggatattg
    43441 ccgaccatta cgcacttggc gtccagcttg gcgttagcgg tactccggca gttgtgctga
    43501 gcaatggcac acttgttccg ggttaccagc cgccgaaaga gatgaaagaa ttcctcgacg
    43561 aacaccaaaa aatgaccagc ggtaaataat tcgcgtgaaa caacagatac aacttcgtcg
    43621 ccgtgaagtc gatgaaacgg cagacttgcc cgcggaattg cctcccttgc tgcgccgttt
    43681 atacgccagc cggggagtac gcagtgcgca agaactggaa cgcagtgtta aaggtatgct
    43741 gccctggcag caactgagcg gcgtcgaaaa ggccgttgag atcctttaca acgcttttcg
    43801 cgaaggaacg cggattattg tggtcggtga tttcgacgcc gacggtgcca ccagcacggc
    43861 tctaagcgtg ctggcgatgc gctcgcttgg ttgcagcaat atcgactacc tggtaccaaa
    43921 ccgtttcgaa gacggttacg gcttaagccc ggaagtggtc gatcaggccc atgcccgtgg
    43981 cgcgcagtta attgtcacgg tggataacgg tatttcctcc catgcgggag ttgagcacgc
    44041 tcgctcgttg ggcatcccgg ttattgttac cgatcaccat ttgccgggcg acacattacc
    44101 cgcagcggaa gcgatcatta accctaactt gcgcgactgt aatttcccgt cgaaatcact
    44161 ggcaggcgtg ggtgtggcgt tttatctgat gctggcgctg cgcacctttt tgcgcgatca
    44221 gggctggttt gatgagcgcg gcatcgcaat tcctaacctg gcagaactgc tggatctggt
    44281 cgcgctgggg acagtggcgg acgtcgtgcc gctggacgct aataatcgca ttctgacctg
    44341 gcaggggatg agtcgcatcc gtgccggaaa gtgccgtcca gggattaaag cgctgctgga
    44401 agtggcaaac cgtgatgcac aaaaactcgc cgccagcgat ttaggttttg cgctggggcc
    44461 acgtctcaat gctgccgggc gactggacga tatgtccgtc ggtgtggcgc tgttgctgtg
    44521 cgacaacatc ggcgaagcgc gcgtgctggc aaatgaactc gatgcgctaa atcagacgcg
    44581 aaaagagatc gaacaaggaa tgcagattga agccttgacc ctgtgcgaga aactggagcg
    44641 aagtcgcgac acgctacccg gcgggctggc aatgtatcac cccgaatggc atcagggcgt
    44701 tgtcggtatt ctggcttcgc gcatcaaaga gcgttttcac cgtccggtta tcgcctttgc
    44761 gccagcaggt gatggtacgc tgaaaggttc cggtcgctcc attcaggggc tgcatatgcg
    44821 tgatgcgctg gagcgattag acacactcta ccccggtatg atgctgaagt ttggcggtca
    44881 tgcgatggcg gcgggtttgt cgctggaaga ggataaattc gaactctttc aacaacggtt
    44941 tggcgagttg gttaccgagt ggctggaccc ttcgctattg caaggcgaag tggtgtcaga
    45001 cggcccgtta agcccggccg aaatgaccat ggaagtggcg cagctgctgc gcgatgctgg
    45061 cccgtggggg cagatgttcc cggagccgct gtttgatggt catttccgtc tgctgcaaca
    45121 gcggctggtg ggcgaacgtc atttgaaagt catggtcgaa ccggtcggcg gcggtccgct
    45181 gctggatggt attgctttta atgtcgatac cgccctctgg ccggataacg gcgtgcgcga
    45241 agtgcaactg gcttacaagc tcgatatcaa cgagtttcgc ggcaaccgca gcctgcaaat
    45301 tatcatcgac aatatctggc caatttagcg tcatcttctc tataaaaaag agcgtggatt
    45361 gggtacaatc ccgctcttat caccgcattt tgactagctc aataaaagaa atcagaccat
    45421 gtttgaaatt aatccggtaa ataatcgcat tcaggacctc acggaacgct ccgacgttct
    45481 tagggggtat ctttgactac gacgccaaga aagagcgtct ggaagaagta aacgccgagc
    45541 tggaacagcc ggatgtctgg aacgaacccg aacgcgcaca ggcgctgggt aaagagcgtt
    45601 cctccctcga agccgttgtc gacaccctcg accaaatgaa acaggggctg gaagatgttt
    45661 ctggtctgct ggaactggct gtagaagctg acgacgaaga aacctttaac gaagccgttg
    45721 ctgaactcga cgccctggaa gaaaaactgg cgcagcttga gttccgccgt atgttctctg
    45781 gcgaatatga cagcgccgac tgctacctcg atattcaggc ggggtctggc ggtacggaag
    45841 cacaggactg ggcgagcatg cttgagcgta tgtatttgcg ctgggcagaa tcgcgtggtt
    45901 tcaaaactga aatcatcgaa gagtcggaag gtgaagtggc gggtattaaa tccgtgacga
    45961 tcaaaatctc cggcgattac gcttacggct ggctgcgtac agaaactggc gttcaccgcc
    46021 tggtgcgtaa gagcccgttt gactccggcg gtcgtcgcca cacgtcgttc agctccgcgt
    46081 ttgtttatcc ggaagttgat gatgatattg atatcgaaat caacccggcg gatctgcgca
    46141 ttgacgttta tcgcgcgtcc ggcgcgggcg gtcagcacgt taaccgtacc gaatctgcgg
    46201 tgcgtattac ccacatcccg accgggatcg tgacccagtg ccagaacgac cgttcccagc
    46261 acaagaacaa agaccaggcc atgaagcaga tgaaagcgaa gctttatgaa ctggagatgc
    46321 agaagaaaaa tgccgagaaa caggcgatgg aagataacaa atccgacatc ggctggggca
    46381 gccagattcg ttcttatgtc cttgatgact cccgcattaa agatctgcgc accggggttg
    46441 aaacccgcaa tacgcaggcc gtgctggacg gcagcctgga tcaatttatc gaagcaagtt
    46501 tgaaagcagg gttatgagga accaacatgt ctgaacaaca cgcacagggc gctgacgcgg
    46561 tagtcgatct taacaatgaa ctgaaaacgc gtcgtgagaa gctggcgaac ctgcgtgagc
    46621 agggaattgc cttcccgaac gatttccgtc gcgatcatac ctctgaccaa ttgcacgcag
    46681 aattcgacgg taaagagaac gaagaacttg aagcgctgaa catcgaagtc gccgttgctg
    46741 gccgcatgat gacccgccgt attatgggta aagcctcttt tgttaccctg caggacgttg
    46801 gcggtcgtat tcagctgtac gttgcccgtg acgatcttcc ggaaggcgtt tacaacgagc
    46861 agttcaaaaa atgggacctc ggcgacatcc tcggcgcgaa aggtaaactg ttcaaaacca
    46921 aaaccggcga actgtctatc cactgtaccg agttgcgtct gctgaccaaa gcactgcgtc
    46981 cgctgccgga taaattccac ggcttgcagg atcaggaagc gcgctatcgt cagcgttatc
    47041 tcgatctcat ctccaacgat gaatcccgca acacctttaa agtgcgctcg cagatcctct
    47101 ctggtattcg ccagttcatg gtgaaccgcg gctttatgga agttgaaacg ccgatgatgc
    47161 aggtgatccc tggcggtgcc gctgcgcgtc cgtttatcac ccaccataac gcgctggatc
    47221 tcgacatgta cctgcgtatc gcgccggaac tgtacctcaa gcgtctggtg gttggtggct
    47281 tcgagcgtgt attcgaaatc aaccgtaact tccgtaacga aggtatttcc gtacgtcata
    47341 acccagagtt caccatgatg gaactctata tggcttacgc agattacaaa gatctgatcg
    47401 agctgaccga atcgctgttc cgtactctgg cacaggatat tctcggtaag acggaagtga
    47461 cctacggcga cgtgacgctg gatttcggta aaccgttcga aaaactgacc atgcgtgaag
    47521 cgattaagaa atatcgcccg gaaaccgaca tggcggatct ggacaacttc gactctgcaa
    47581 aagcgattgt tgaatctatc ggtatccacg ttgagaagag ctggggtctg ggccgtatcg
    47641 ttaccgagat cttcgaagaa gtggcagaag cacatctgat tcagccgacc ttcattactg
    47701 aatatccggc agaagtttct ccgctggcgc gtcgtaacga cgttaacccg gaaatcacag
    47761 accgctttga gttcttcatt ggtggtcgtg aaatcggtaa cggctttagc gagctgaatg
    47821 atgcggaaga tcaggcgcaa cgcttcctgg atcaggttgc cgcgaaagat gcaggtgacg
    47881 acgaagcgat gttctacgac gaagattacg tcaccgcact ggaacatggc ttaccgccga
    47941 cagcaggtct ggggattggt atcgaccgta tggtaatgct gttcaccaac agccatacca
    48001 tccgcgacgt tattctgttc ccggcgatgc gtccggtgaa ataagcatta cgttatgcgc
    48061 ataaccccgg cagatgtcgg ggttttttaa ttgtgctgcg cgaaagcaga caacaatttt
    48121 cttgcttcac tattggctgc ctgcatcacc atccacggac tgaacgccca cggcgtggca
    48181 tcaataccgt gtaatacatc tgctaaatca caccattgat aatccatcac ttcatcatcg
    48241 ttgatctgta acgcactggt ggtgcgtgcg gcaaataccg gacacacttc attttccaca
    48301 atgccattcg gatcggtggc gcgatagcga aagtcaggat agatagattc aggaggcgta
    48361 atttccacgc caagctcata acggcaacgg cggatcaccg cgtcttcgtt gctttctccc
    48421 agttgtgggt gcccacaaac cgagttagtc cacacgccag gccatgcttt tttgctcagt
    48481 gcgcggcggg taactaataa ttgtcctttg gcattaaaca gccaactgga gaacgcgaga
    48541 tgtaagcggg tgtctgccgt gtgtgcggca tacttttcca gcgtacccgt gggaactccc
    48601 tgtgcattca ataaaatgac gtgttccgtt tgcataattt ctcacatgta attctgatca
    48661 ctcatcatta tagataattg aagagtgaaa tgattaacca agcgagcgtt tatcgacaac
    48721 cgcccgtttt gagcggatgc cgacaggtaa aaaccattta aaaaagtagc atcatatatt
    48781 tgaagcgact ttcaatggcc attgcaacac agtcagaaat gacaggaata gagagagtaa
    48841 aaagcaacag cagactgaat atagcgcgca ttaaaaaatt catattattc tccatgctca
    48901 tttttcttca gcttgcgcat cagaagccac atacgtactg tcctgacttt taaatcttta
    48961 tcagagacgg attccttcac caggtcagca tgatgctgat agccattaaa gaagacgttc
    49021 agcacaacgg cgctaagggt tgcgagcata atgccgctat gtagcagcgg ttgcagtacg
    49081 gccggtaact tagaaaagaa atcgtgagag agcgttggag tcatgccaac gccgagactg
    49141 atcgccacaa tatagaggtt gtaacggttg gtggtgtagt tacagcgcga cagaattcga
    49201 atccctgtcg ccagtaccat gccgaacatc accagaccag cgccgcccag cacaaattgc
    49261 ggaatggagg ctaccagcac cgccattttt ggcaccatgc caaacaggat taatataatt
    49321 cccgaagaaa tacacaccca gcggctatga acgcgcgtca cgctaaccag gccaacgttt
    49381 tgagaaaatg acgtgtgggg gaagctgtta aacgtgccac caatcattgt ccctacgcca
    49441 tcgacacgca gcccgcgaat aatatcgtgc gaagagagtt tacgaccgac tatttcaccc
    49501 agtgccagga acatccccat cgactcgata aacacgatga ttaacacggc agtcatggtc
    49561 agaatggaaa cgggatcgaa aatcggcata ccaaacgaca tcggggtgac aatcgcaaac
    49621 cacgaggcat catgtagccc ggataaattg acttcattca tcatccacga aagtaaaaag
    49681 ccaaatacaa tccccagtaa tacggcgacg ttggacataa atcctttcgc atagcgagta
    49741 atgagcaaga taaaaattaa gacggcaaag gagataccta aataaacggg attaccatat
    49801 tgcggattcc ctttacctcc ggcggcccag tcaataccca cctgaatgat gctaagcccg
    49861 atagaagtaa taaccacacc ggtaaccagt ggcgggaata aaggcatcaa gcgaccgata
    49921 agttgcgcta ataatgtggt gataaaaccc gcggcgatag tggcaccaaa tatacccagc
    49981 aggccgatat ccgggttcat cccaatggct atcattggtg ttacagcagc aaaggtcacc
    50041 gacataatca ccggcaggcg gatccccata aagcggccga taccgataca ttgcaataat
    50101 gtgacgatcc cgcagcaaaa gagatccgag ctaatgagca tcgcaatagc ttcttttgag
    50161 aggcccagtc ggtcaccaat cataagagga acagcgactg cacctgcgta catgacaagg
    50221 acgtgttgca gaccgagtat gatcagcttt cctggtgata atatgcggtc aacctcatca
    50281 gttgggcggt cttgcccaga agatgaggga agttgggaat ctatggcgct catcagtgtt
    50341 tctcctttcg cccgccagag taaaaagcaa ccgtttacgt taatgaaact gtctaaacac
    50401 atgctcgtgg agtgccagtt tttgcgagga aggggagtta gagacaggaa ttgcggaggc
    50461 aatcacataa tcgtgcttaa actacgaaaa tgacaagaga acctgacaat ggaattatct
    50521 gtaattttct catattgata tttcattaat cattctggca tgtcgcactc tcgcatttaa
    50581 tcgtttttat ctggatagcg ctatttgggg cggcaatgtt gaaataatga aagcggcata
    50641 gattgagggg caataatttg aactcagtcg caaaattata attaaaacgg tcatgctacc
    50701 taatatatgc ccgtttattg catcgcgaaa tttgagaaga cataactcat taaatgaaag
    50761 ggatgcaaat gaataagttt atcgctgctg aagccgcgga atgtataggc tgtcatgctt
    50821 gtgaaattgc ctgtgcggtg gcacacaatc aagaaaactg gccgctgagt cacagtgact
    50881 ttcgaccacg tatccacgtt gtagggaaag gcctggctgc gaatccggtg gcctgccatc
    50941 actgcaacaa tgccccttgc gttacggctt gtccggttaa tgctctgact ttccagtccg
    51001 atagcgtaca actggacgag caaaaatgta ttggttgtaa aagatgcgca atcgcttgcc
    51061 cctttggcgt cgttgagatg gtcgatacga ttgcgcagaa atgcgacctt tgtaaccagc
    51121 gcagttccgg cacgcaagcc tgtattgaag tttgcccaac acaggcgtta cgactgatgg
    51181 acgataaagg gttacagcag ataaaggtgg cccgccagcg caaaacggca gcaggaaaag
    51241 cgtcatcaga cgctcagcca tctcgcagtg cagcgttgct ccccgttaac tcgcgtaaag
    51301 gcgcagataa aatttcagca agtgaacgga aaaaccactt tggcgaaatc tattgcgggc
    51361 tggatccaca acaagcgact tatgagagtg accgctgtgt ttattgtgcc gaaaaagcta
    51421 actgcaactg gcattgcccg ctgcataacg ctattccgga ttacatccgt ctggtacagg
    51481 agggaaagat tattgaagcg gcagaacttt gccaccagac cagttcctta ccagaaatct
    51541 gcggcagggt atgtccacag gaccgtcttt gtgaaggcgc atgtactttg aaagatcact
    51601 ctggcgcagt ctctatcggt aatctggaac gctacatcac cgataccgcg ctggcgatgg
    51661 gctggcgtcc cgatgtcagc aaagttgttc cccgtatcga gaaagtggcg gtgattggcg
    51721 ctgggcctgc agggttaggg tgtgctgata ttctggcgcg cgcaggagtt caggtcgatg
    51781 tctttgatcg ccatccagaa attggcggta tgctgacttt tggcattcct cctttcaaac
    51841 tcgataaaac ggtattaagc cagcggcgag agatattcac cgcaatggga atcgactttc
    51901 atcttaactg tgaaattggc cgcgatatta cctttagcga tttaacttct gaatatgatg
    51961 cagttttcat cggcgtgggg acttacggga tgatgcgagc agatctgccg catgaagatg
    52021 cgcccggtgt cattcaggct ctaccgttcc tgactgccca tacccgccag ctcatgggat
    52081 tgccggagtc tgaagagtat ccgctgacgg acgtggaagg taagcgagtc gtggtattgg
    52141 gcggtggcga tacgacaatg gattgtttgc ggacttccat tcgcctcaat gctgccagcg
    52201 tgacctgcgc gtatcgtcgt gatgaagtca gtatgccggg ctcgcgcaaa gaggtggtca
    52261 atgcgcgcga ggaaggtgtc gagtttcaat tcaatgttca gccgcaatat atcgcttgtg
    52321 acgaagatgg gcgtttaact gcggtgggcc tgattcgtac cgccatgggt gagccggggc
    52381 cggatggtcg ccgtcgtcct cgtccggttg cgggttcaga gtttgaattg cccgccgatg
    52441 ttctcattat ggcctttggt ttccaggcgc atgccatgcc gtggttgcag ggcagcggaa
    52501 ttaaactcga taaatggggc ctgattcaaa ccggtgacgt cgggtattta cctacccaga
    52561 cgcatctgaa aaaagtcttt gctggtggtg atgcagttca tggcgcggat ctggttgtca
    52621 ctgcaatggc cgcaggaagg caggcggcgc gcgatatgtt aactctgttt gatacgaagg
    52681 catcgtgatg aaatcgttaa ttatcgttaa tccggctgac tgtattggct gccgtacttg
    52741 tgaagtggcc tgtgtggtcg ctcacccttc ataacaggag ttgaatgccg atatcttttt
    52801 accccggctg aaggtacagc gactggactg catcagtgcg ccggtgatgt gccatcagtg
    52861 tgaaaacgcc ccttgtgttg gcgcttgccc cgtgggggcg ctgacgatgg gcgagcaggt
    52921 ggtgcagacc aattctgccc gttgtattgg ctgtcagagt tgcgtcagtg cgtgcccgtt
    52981 tggaatgatc accattcagt cgttgccggg ggatacccgg caacaaatcg tgaaatgcga
    53041 tctctgtgaa cagcgggaag aggggcctgc ctgcgttgaa tcttgcccaa cacaggcgct
    53101 gcagttgctt actgaaagag aactcaggcg agtccgccag cagcgtattg ttgccagcgg
    53161 tgagaatcct ctgtgaaaga aatctccgcg ctgaaaggcg cggagaatct attagattgc
    53221 ccaaccaccc gcgtaaaatg cgaccagtgc ggcagtaatg atcaccgtac caatattcag
    53281 cttttgccat tcgcgtgcaa agacgcggcc tacgaccagt gtcacaaagc ccagcataat
    53341 accggtaacg atattacaag tcagaacgat gaacacggca cacaccaggc cagccatagc
    53401 gtcaataaaa tcattgaaat ccagcttcga gacgttactt aacatcagca aacctacgta
    53461 catcagagcg ggtgcagtgg cgtaaccagg gatcaaaaat gacagcggtg ataagaacag
    53521 aatcagcagg aataacgccc ccactacggt tgcggttaac cctgttttac ccccggcggc
    53581 ggttcctgcc gctgattcga tataaaccgc tgcgggcgct gcgcccacca ggccggagaa
    53641 tattgaactt actgagtcac tggtcagggc tttgccgccg ttgatgatct ggttgtcttt
    53701 atccaacaaa ttcgcctgac cggcgacggc acggatggtg ccagtagcgt cgaacactgc
    53761 ggtcatcacc aatgccagta cactcggaag tacagttggc tggagtgcgc ccataatatc
    53821 gaggctgaaa atcagagact taccatcttc gccagtcagg cttggcatcg ccaccagacc
    53881 gtggtatttc accgctgggt caaagattaa gccgatgatc gaaattgcaa taatcaccaa
    53941 caagataccg ccgggtacgc gacacttctc cagaccgaag atgaccgcca gccccagcaa
    54001 gctcatcatc accgggaagg aggtaaacgc accgagcgcc actggcaagc cttcaatcgg
    54061 gtttttgata accataccca caccgttagc agcaatcagc agcagaaaca gcccgatacc
    54121 gatacctgta ccgtgagcga tacccatcgg caaattacgt aagatccagg tacgcacacc
    54181 ggttacggaa atggcggtga agatgacgcc catcagaaat accgcgccca gtgcgacagg
    54241 aacgctaatt tgttgcccga gtaccagact gaatgcggta aacgccgtca aggaaatcgc
    54301 gcaaccaatc gccattggca aattagccca taaccccatc agcaacgagc cgaagcccgc
    54361 gaccagacag gtggcaacaa acacagctgc gggaggaaaa cctgctttgc ccagcattcc
    54421 cggaacgacg ataacggaat aaaccatggc cagaaaggtc gttaagccag ccagtacttc
    54481 ctgacgaacg gtactgccac gagcggtaat tttaaaataa ttatccagcg cgccctgtgg
    54541 ctttggtgcg tccggtgttt gtaggatgtc tccagacata atgatgtcct cacgaggatg
    54601 taaaagtttt attattagtt gcgttcgtac accagacgac catcaacgta ggtgcggtag
    54661 atcgaacggt catcgcccaa cgtcatcatc acgaacaatt tgtcgactaa agaaacagag
    54721 ttgtcatagc gcagctgctg tagcggagtg gcggtgggtt ccatcaccac gaaatcagcc
    54781 tctttgccag gtaaaaagtt gccaatcaaa tcgtcaaggc ccagagattt cgctccgccg
    54841 agcgtggcca ggtaaaacgc ttcatatgcc gagaggcgat agccttgtaa ttgcaacact
    54901 ttgtaggctt cgttcagcgt ttgcagcatg ttgaaagtgg ttccggcacc gatatccgtt
    54961 cccatgccca ctttaacttt cttctgccat gcttttttca agttgaataa cccgctgccg
    55021 aggtaaaggt tggaggtcgg acagaaagca atgctggatt tggtttcgct gagacgatcc
    55081 cactcttttt ctttgagatg gacgcagtga gcaaagacac agtttttacc ggtcaggccg
    55141 tactgatggt aaacatccag ataaccatca tggtcaggat aaagcgattt cacccaggca
    55201 atttcatctt tgttttcaca gagatgggta tgtacccacg tatccggata ttcctctttc
    55261 aggcgttgcg ccatcgccat ctgttcagga gaagaagtag gggcgaagcg tggcgtaatc
    55321 gcatatagca gacgaccatt tttgtgccag cgttcaatca gttctttgct ttggtgatag
    55381 ctgctttcgg cagtgtctag cagataatcc ggtgcgttgc ggtccatcat caccttaccg
    55441 gcaatcatac gcatattgat atgactggcg gcttcaaaca gcgcatcaac ggattgcgga
    55501 tgaacagtgc caaacaccag cgccgtggtg gttccgttac gtaaaagctg cttgatgaag
    55561 aacgccgaca tttcgcgggc gtactctaaa tcctcataac gacgttcagt agggaaggtg
    55621 tgtttattca accactccag caattgttcc ccataggccc ccaccatttc actttgcgga
    55681 taatggatat gtgtatcgac aaagcccggt actatcagtt tgccgcgata gtcgcgcacg
    55741 cgaatagtgt caggaatttg atgctttccg tcttcccatt cgccaaacca ttccactttt
    55801 ccctgtttaa tgagtaataa accatcctca ataaaccgca gcgcagaggc aatctcttcc
    55861 gggttatcga ccgtacgggc gacatcaata aaactgcctc gtaccgcttt taacgtgtgt
    55921 tctcctgaca tcattgactc ctttactgtt aatccatctc ttccgctgag gtaataccag
    55981 gcagaacgtt ttcctgtcgg tagccaccag ggaggataat atttaataaa atcgcagtta
    56041 acccgccagc acaaa
//
LOCUS       DBFIFM010000007        55012 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.007, whole genome shotgun sequence.
ACCESSION   DBFIFM010000007 DBFIFM010000000
VERSION     DBFIFM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.007"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(13..2325)
                     /gene="pbpC"
                     /locus_tag="N7C74_000397"
     CDS             complement(13..2325)
                     /gene="pbpC"
                     /locus_tag="N7C74_000397"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311412.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HGU2430338.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEVA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEALPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGEIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIASVHFSFQ"
     gene            complement(2326..7287)
                     /gene="yfhM"
                     /locus_tag="N7C74_000398"
     CDS             complement(2326..7287)
                     /gene="yfhM"
                     /locus_tag="N7C74_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HGU2430339.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKAGYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADEFGSNESKVIVAAPVIAELNMPRF
                     MASGDNSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRDEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLATLQVTNSGEQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            7494..8339
                     /gene="sseA"
                     /locus_tag="N7C74_000399"
     CDS             7494..8339
                     /gene="sseA"
                     /locus_tag="N7C74_000399"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HGU2430340.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPLK"
     gene            8682..8807
                     /gene="timP"
                     /locus_tag="N7C74_000400"
     CDS             8682..8807
                     /gene="timP"
                     /locus_tag="N7C74_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HGU2430341.1"
                     /translation="MKIRCFCIVLIVSGALLTEVNNNRSLSGDNLLVINNLQSSR"
     gene            complement(8839..9615)
                     /gene="sseB"
                     /locus_tag="N7C74_000401"
     CDS             complement(8839..9615)
                     /gene="sseB"
                     /locus_tag="N7C74_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HGU2430342.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQVAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(9758..11041)
                     /gene="pepB"
                     /locus_tag="N7C74_000402"
     CDS             complement(9758..11041)
                     /gene="pepB"
                     /locus_tag="N7C74_000402"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HGU2430343.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVASDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(11219..11419)
                     /gene="iscX"
                     /locus_tag="N7C74_000403"
     CDS             complement(11219..11419)
                     /gene="iscX"
                     /locus_tag="N7C74_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HGU2430344.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(11431..11766)
                     /gene="fdx"
                     /locus_tag="N7C74_000404"
     CDS             complement(11431..11766)
                     /gene="fdx"
                     /locus_tag="N7C74_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HGU2430345.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(11768..13618)
                     /gene="hscA"
                     /locus_tag="N7C74_000405"
     CDS             complement(11768..13618)
                     /gene="hscA"
                     /locus_tag="N7C74_000405"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HGU2430346.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(13635..14150)
                     /gene="hscB"
                     /locus_tag="N7C74_000406"
     CDS             complement(13635..14150)
                     /gene="hscB"
                     /locus_tag="N7C74_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HGU2430347.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(14246..14569)
                     /gene="iscA"
                     /locus_tag="N7C74_000407"
     CDS             complement(14246..14569)
                     /gene="iscA"
                     /locus_tag="N7C74_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HGU2430348.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(14586..14972)
                     /gene="iscU"
                     /locus_tag="N7C74_000408"
     CDS             complement(14586..14972)
                     /gene="iscU"
                     /locus_tag="N7C74_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HGU2430349.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(15000..16214)
                     /gene="iscS"
                     /locus_tag="N7C74_000409"
     CDS             complement(15000..16214)
                     /gene="iscS"
                     /locus_tag="N7C74_000409"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HGU2430350.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(16326..16814)
                     /gene="iscR"
                     /locus_tag="N7C74_000410"
     CDS             complement(16326..16814)
                     /gene="iscR"
                     /locus_tag="N7C74_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HGU2430351.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(17115..17852)
                     /gene="trmJ"
                     /locus_tag="N7C74_000411"
     CDS             complement(17115..17852)
                     /gene="trmJ"
                     /locus_tag="N7C74_000411"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HGU2430352.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            17971..18774
                     /gene="suhB"
                     /locus_tag="N7C74_000412"
     CDS             17971..18774
                     /gene="suhB"
                     /locus_tag="N7C74_000412"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HGU2430353.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            18919..19773
                     /gene="yfhR"
                     /locus_tag="N7C74_000413"
     CDS             18919..19773
                     /gene="yfhR"
                     /locus_tag="N7C74_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU2430354.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVNFI
                     LSALNPQN"
     gene            19964..21244
                     /gene="csiE"
                     /locus_tag="N7C74_000414"
     CDS             19964..21244
                     /gene="csiE"
                     /locus_tag="N7C74_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HGU2430355.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(21236..22375)
                     /gene="hcaT"
                     /locus_tag="N7C74_000415"
     CDS             complement(21236..22375)
                     /gene="hcaT"
                     /locus_tag="N7C74_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HGU2430356.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(22535..23425)
                     /gene="hcaR"
                     /locus_tag="N7C74_000416"
     CDS             complement(22535..23425)
                     /gene="hcaR"
                     /locus_tag="N7C74_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HGU2430357.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            23561..24922
                     /gene="hcaE"
                     /locus_tag="N7C74_000417"
     CDS             23561..24922
                     /gene="hcaE"
                     /locus_tag="N7C74_000417"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HGU2430358.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            24919..25437
                     /gene="hcaF"
                     /locus_tag="N7C74_000418"
     CDS             24919..25437
                     /gene="hcaF"
                     /locus_tag="N7C74_000418"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HGU2430359.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEICYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            25437..25757
                     /gene="hcaC"
                     /locus_tag="N7C74_000419"
     CDS             25437..25757
                     /gene="hcaC"
                     /locus_tag="N7C74_000419"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HGU2430360.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            25754..26566
                     /gene="hcaB"
                     /locus_tag="N7C74_000420"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             25754..26566
                     /gene="hcaB"
                     /locus_tag="N7C74_000420"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HGU2430361.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIAGEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            26576..27778
                     /gene="hcaD"
                     /locus_tag="N7C74_000421"
     CDS             26576..27778
                     /gene="hcaD"
                     /locus_tag="N7C74_000421"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HGU2430362.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTRELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANDQLAREANLDTTNGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNHAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRSIRKWIQSGKTFDAKQLTD
                     ENIALKSL"
     gene            27875..28297
                     /gene="yphA"
                     /locus_tag="N7C74_000422"
     CDS             27875..28297
                     /gene="yphA"
                     /locus_tag="N7C74_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HGU2430363.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(28345..29217)
                     /gene="yphB"
                     /locus_tag="N7C74_000423"
     CDS             complement(28345..29217)
                     /gene="yphB"
                     /locus_tag="N7C74_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HGU2430364.1"
                     /translation="MTIYTLSHGSLKLGVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIIETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(29229..30323)
                     /gene="yphC"
                     /locus_tag="N7C74_000424"
                     /pseudo
     CDS             complement(29229..30323)
                     /gene="yphC"
                     /locus_tag="N7C74_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
     gene            complement(30356..31354)
                     /gene="yphD"
                     /locus_tag="N7C74_000425"
     CDS             complement(30356..31354)
                     /gene="yphD"
                     /locus_tag="N7C74_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2430365.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSHHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(31379..32890)
                     /gene="yphE"
                     /locus_tag="N7C74_000426"
     CDS             complement(31379..32890)
                     /gene="yphE"
                     /locus_tag="N7C74_000426"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGU2430366.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGEIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAHKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVIRAVKKMSALGVAVIYVSHRMEEIRRIASS
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(32913..33896)
                     /gene="yphF"
                     /locus_tag="N7C74_000427"
     CDS             complement(32913..33896)
                     /gene="yphF"
                     /locus_tag="N7C74_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGU2430367.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(33993..37274)
                     /gene="yphG"
                     /locus_tag="N7C74_000428"
     CDS             complement(33993..37274)
                     /gene="yphG"
                     /locus_tag="N7C74_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HGU2430368.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTRLDAYEE
                     KRFEQFFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCD
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLSAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPINQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWCLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNEAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGTTPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICAKAQGDETEATRCLHLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLAAAGLGEIAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            37392..38585
                     /gene="yphH"
                     /locus_tag="N7C74_000429"
     CDS             37392..38585
                     /gene="yphH"
                     /locus_tag="N7C74_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGU2430369.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGEHWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVET
                     QLRQI"
     gene            complement(38648..39901)
                     /gene="glyA"
                     /locus_tag="N7C74_000430"
     CDS             complement(38648..39901)
                     /gene="glyA"
                     /locus_tag="N7C74_000430"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HGU2430370.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEVEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            40229..41419
                     /gene="hmpA"
                     /locus_tag="N7C74_000431"
     CDS             40229..41419
                     /gene="hmpA"
                     /locus_tag="N7C74_000431"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HGU2430371.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(41464..41802)
                     /gene="glnB"
                     /locus_tag="N7C74_000432"
     CDS             complement(41464..41802)
                     /gene="glnB"
                     /locus_tag="N7C74_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HGU2430372.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(41863..43197)
                     /gene="glrR"
                     /locus_tag="N7C74_000433"
     CDS             complement(41863..43197)
                     /gene="glrR"
                     /locus_tag="N7C74_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HGU2430373.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(43187..43900)
                     /gene="qseG"
                     /locus_tag="N7C74_000434"
     CDS             complement(43187..43900)
                     /gene="qseG"
                     /locus_tag="N7C74_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HGU2430374.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(44065..45492)
                     /gene="qseE"
                     /locus_tag="N7C74_000435"
     CDS             complement(44065..45492)
                     /gene="qseE"
                     /locus_tag="N7C74_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HGU2430375.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELDNVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(46068..49955)
                     /gene="purL"
                     /locus_tag="N7C74_000436"
                     /gene_synonym="purI"
     CDS             complement(46068..49955)
                     /gene="purL"
                     /locus_tag="N7C74_000436"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HGU2430376.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            50212..51768
                     /gene="mltF"
                     /locus_tag="N7C74_000437"
     CDS             50212..51768
                     /gene="mltF"
                     /locus_tag="N7C74_000437"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HGU2430377.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMQ
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(51765..52268)
                     /gene="tadA"
                     /locus_tag="N7C74_000438"
     CDS             complement(51765..52268)
                     /gene="tadA"
                     /locus_tag="N7C74_000438"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HGU2430378.1"
                     /translation="MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(52326..52961)
                     /gene="yfhb"
                     /locus_tag="N7C74_000439"
     CDS             complement(52326..52961)
                     /gene="yfhb"
                     /locus_tag="N7C74_000439"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HGU2430379.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            53170..54018
                     /gene="yfhH"
                     /locus_tag="N7C74_000440"
     CDS             53170..54018
                     /gene="yfhH"
                     /locus_tag="N7C74_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGU2430380.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            54074..54334
                     /gene="yfhL"
                     /locus_tag="N7C74_000441"
     CDS             54074..54334
                     /gene="yfhL"
                     /locus_tag="N7C74_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HGU2430381.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            complement(54528..54608)
                     /gene="shoB"
                     /locus_tag="N7C74_000442"
     CDS             complement(54528..54608)
                     /gene="shoB"
                     /locus_tag="N7C74_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HGU2430382.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
BASE COUNT        13015 a        15163 c        13875 g        12959 t
ORIGIN      
        1 attttgtata tttcactgaa atgaaaaatg tactgacgcg atttgcccta cgtcatccat
       61 caccagcaat tgataatcac ctttatccgt caaatgcagg gtcacgttgc gcccgcgttc
      121 agttaacggt tcgccattca gaaaccacca gcgttcacct gcccctccac ttgattgcaa
      181 cggcaaagtc gcttccgctg cgcccggtaa acgtttaata atctcgccat cgcgaacgcc
      241 tgtcagctgc agcgggagct gggcatcgtg accgtatggc gggcaaattg tcgaggccgg
      301 tggtaagcgc acagcgcgac gttctgatgc gggcaaccag ggttccagcg gtagcggcca
      361 gacatttatc atttcttgtc gcgcttgcgg gcaatcagcg gcaacacgtt tgccattttc
      421 atccagccag atggggaaac gaatgccatt aatgccttcc tgctccggca gtaatagagt
      481 tggcggctga ctcccgtcca gcagccaggt tgccaggcgg cggcgacagt taccgtcacc
      541 ttccggcaaa gactgtccgc ccggccagca aataacgcca cgagtgactg agttcgggcg
      601 cgggtcttcc ggcagattag cactgcgcga cagtaagata ttattgacct gattcaacaa
      661 tggtacggcg ctggcaaaac caaactgacc aacaacgggc gtgccgtccg gtctgccagt
      721 ccagatccca atgacatagc gagcgttaac cccaatcgcc caggcgtcac gatagccata
      781 gctggtgccc gttttccatg ccagtggggc gacgcgcggc aaggcaccat ccggcaaggg
      841 tagcgcttca tcagccataa tccggcgaat gatccacgcc gctcccgacg acattaaagg
      901 ccgttcaagc agcggatcgt caggctgtaa gcgcaatttg cctgccttgc cgtggcgagc
      961 aaacgcggta tacgctgccg ccatatcttc cagtttcgca ccagcgccgc cgagaatgag
     1021 cgaaagattc ggcgcagcac cgttgggcaa atataacggc aatccaacat tgcgtaactt
     1081 tgccgcaaac cgtttcggtc cataggcttc cagcacctgc acagcaggta aattcagcga
     1141 gcgcaccagc gcctcgctca tgctgatcgg gccatgaaaa ccgctgtcaa agttgccagg
     1201 tcggtaatca ccggtacgcc gggggacgtc ttgcagcagt gatgccgggt ggatcaatcc
     1261 ttcatccagc gccagaccgt aaacaaacgg tttgagcacc gatcctggcg atcggatcgc
     1321 attgaccata tcaacatgac caaagcgtga atcatcgttg agatcaaccg aacccaccca
     1381 gccgcgaaca cgcatatcgg tatgatcaac cacgatcatc gccagtgagc tgcgcggtgg
     1441 taatcgtcct ttccagtttt gcgccagttc ttccagacgt cgttgaagac cggcatccag
     1501 cgtagtaacg attttgtcgc ttttgctttt accgagcatc atgcgcgaaa acagcggtgc
     1561 cagttgcggc atttgtcggg gtgccagcca gatgggttct tcccttgact cttttacctg
     1621 ctcacgggac cacacacctt gcacggccat ccgttcgagc actttattac gcgcgacttc
     1681 ggcacgctcc ggccaacgat ccgggcgaag acggctgggc gcttgcggca aaaccgccag
     1741 cattgccgcc tcggaatagc ttaaattcgc aggcgacttt ccgagataag cccaacttgc
     1801 cgcaccgatc ccctgcaacg taccgccaaa cggagcgcgg ttaagataca aggtcagaat
     1861 ttcacgctta gacagatgcc attccagttg caacgcgcgc cagagctggc gaattttgcc
     1921 gccaaatgtt ttggggtgag gatcaagcag acgagcaacc tgcatagtga gcgtgctgcc
     1981 accggaaata acccgtcccg aagtgagatc ttgccatgct gcgcgcgcca ccgagaatgg
     2041 attcacccca ggatgcttcc agaaccagcg atcttcataa ttgatcagcg cttcaaggta
     2101 acgtggagaa acatcttcga ttgttacagg ataacgccag atgccgtcag catcggcgaa
     2161 gcgccagagc ggcgtaccat cctgcgccac gaccactcgt gcgggattga cttcatgcag
     2221 cggtagaggc cagagtttat ctgctcccca cgctgcaaga aaaaggagaa atggcgcagc
     2281 tgccagcgtt atccagcagc cgcgtttggt taacaagcga ggcatttacg gtctgacaat
     2341 cagcaaatct tcagccgcgc cggtcgcccg ccattgggga acatacattg attccaccat
     2401 cggttgcggt acctgatacg ttcccggcgt caccgcccgc gccagataca ccagcgttac
     2461 cggttggtat tcatcaacgg caaccgccgc cacaaagcga tcgtcacgga actcaatgtg
     2521 cttaatactc gcctgctgca tctggttcag taagttctgc acttcgccac cactttgctc
     2581 caggctggcg ctaccgttcg ccagattctg gttttccagt tccagacccg caggcagcag
     2641 atccacgact aacgcatccg gcacgctgtt actagctttt acctgcaacc agaccagcac
     2701 cagatcgccg ctacgtaacg agtccagcga tttgctctta ccatcagtac caagaatatg
     2761 acgctcaatt tgcagcacat tgttcgccgg taaaggtgcg gattgcggat aaccgctggc
     2821 atccacacgc agccataacg gctgctcgcc actgttggtc acctgcaagg tggcaagttg
     2881 atcgctattc agattgctgt tttgcgcttt ctcgcctgtc agcggctcag ctgagaaaga
     2941 ggtttgcgcc tgccatttac ctggtaaatc ctgaatcgta cgggcagcca ggaacaacgc
     3001 gttactttcc tgcgtcgata gccagcgttc accaaacgcc tgctgcgaaa gtgtgttcag
     3061 cagggagtac tgctcatcgg gtagcagttt gttctcttcc agcaaggaga gcatcaacgc
     3121 gttgtcgcgc agtggactac cgtaatcacc cagccatatc cgctcatcac tattacgcgg
     3181 cgtttttagc gccagcgcaa tcgcttcttc atcacgcgtc gcatcaccca tggttttcag
     3241 cgcaacgcca agttgcagca gcggtaaacc agaagcggca tctgcacgat gctcccagat
     3301 ttcacgcagc gcacccagcg gagccttttg ctgacgggcc aacaccagcg cagcgtaaga
     3361 ctgtacggcg aatttactgg ctttgagatt atccgcgtac gggatcgaca tcatgcccgg
     3421 atcttgtaaa tagcgcagca gacgctcatt accccggtta atggcgtctg tcggcacgct
     3481 gtaaccctgt tcgcctgcgc ggaccaggaa atccatcacg taagccgtca gccagtactc
     3541 ttcgtcaccg tttttatccc acagcgcaaa gccgccgtta tcacgttgca tttgcagcaa
     3601 acgggaaatg ccgatatcga ccgatgcacg gcgtttctca tcactgtcgc ctttgatgcc
     3661 caacgcctgc agttgggctg cgttggtata aagtgacggg aacaggccgc tggcggtttg
     3721 ctcaagacag ccgtacggat acgcttttaa ctctttgata taacgtgcga tgttcagcgg
     3781 tggtttgccg ctcaacaaca attgcccttc cagcgtaaca ggcgagaagt tttgcaatcc
     3841 atccgccgga atcgcccatg tctcaccagg ctgtaacgcc gtaccgtaat taaccgtttg
     3901 tgccgggaac gccggacgga cgccgatttt ccactgctta tgctgatcgg caacggtttc
     3961 acccggtaac gctaacccgc taatggtggc ctgaatttct ccatcgccat aacccggcaa
     4021 tgctcgcacc gggataaaca gcgtagtacg cacacctggc gctaattcaa cggctgcggg
     4081 tgaatcgctg accagttcaa gcaaaccact ggcggtcagg gcaacgttca gtttttgcgg
     4141 tttatcggta agattagtga tatccagcgt cagacgcgag ttatcgccac tcgccataaa
     4201 gcgcggcatg ttcagttcag caatcaccgg tgcggcaact atcactttgc tttcgttgct
     4261 accgaattca tctgccgtcc aggcttgcgc catgacgcgc aattcaccgt taaaatcgcc
     4321 aatcggcagt gtaaccgagc cttcgccctg ttcgttgagc gttaccggca gcgcctgctg
     4381 cgcgacaata ttgacgtgat tgaccggcgg tttaccacca cgtttcagct catcaccatc
     4441 gccaccgaaa cgcagagctg ccagacgccc ctgaccttca ataacctgac cgtaaatatc
     4501 gtaaatgtct gcgccatagc gtttctgacc aaagaacgcc tgccacggat ctggcgtgac
     4561 gtagtcagta atattcagca caccactatc aacggcagac accagcacat tcacctgttt
     4621 aggcttctcg ccatttttag tgctggcttt aattttcacg gttaatggtt gattgggacg
     4681 catttttgct ggtgtttcca gcgccagatc gaggcgacgg ttttcatcac caagcggcag
     4741 atgcaacaca ccaaccgcgc gttttggcgt cgcggagcga gatttatcgc caggacgcac
     4801 caccagcgta ctcaaataaa gatcatgacg attccaggtt ttatcgaccg gaatcgtcag
     4861 atccagccct tgagccggaa catcaatctc ttgccaccac agcggccctt cactggactc
     4921 gaccatcgca taacctttac ccgccgttgg cgcggcgata tgcaacttaa tggtatcgcc
     4981 agggcgataa ccggctttat ccagtttcag cgtgacacgg tcgggtcgca ctgcgccgct
     5041 accgtcgcta ttatcctgcc agctatagcc agcccaaaaa cgaacactac tgaccgcttc
     5101 attcggcgct ttgacttcca gacgataagc gccccactct accgggaaac tgaccttgcc
     5161 ggtttcgtcc gctttcagat ccagagtttg ttcattttca atcagatctt tttgatcaaa
     5221 ctgtgattgc cagccttcat cttctgacca gttccagtag taatcgcggc gttcgcgaat
     5281 caggcgcacc tgcaagcccg acacggcttt tttcacgcct tgcgcatcgc tataaacgat
     5341 gtcaaaagcg gcgttactgc cttcatcaac aatgggctgt ttcaccgtgc tgtcagtgcg
     5401 ataatcgtaa accgatttcg aggcgaactg cggacggatc cccggcaatg catcggcagg
     5461 ccagatagcc tgctcagcgc ggcgcgtcac cgggcgaccg cccgattcca gcaaactgcc
     5521 ctggaaaata acctgcaatg gggaatgcgt ttccttccac tggctttctg tagaaacttc
     5581 gccgcgccct ttatcatcca gcgtcaactg aacttcatcc agcgtgcggg aaaggttttc
     5641 ggcagctata tcgccgaatt caaaaccagg taaggctgac acagcctcac gcagtgggcg
     5701 caggaaaagt tgcccttgca aagtattacc attagcaggc gcaccgtaca ggtagtatcc
     5761 caccacggag aatttcactt catcattcgg cgtaagcggg gttttctcac cggtcagatt
     5821 cagcgccata cgctctggca taaaatcttc gacgtggaaa tcccacatcc gatactggtt
     5881 atcgcccgtg ttagcgcgaa tatgccacat accggttgcc gcattgctat cgagtggcca
     5941 ggtaaagtgg tagaggccat tttccggctg actaacgacg ctcctgagta cctgcccatc
     6001 gggtttaatc acgtctaact tgatgggttg attgggcaac gctttaccgt ctgcatcacg
     6061 cagcaaacca ttgaggatta ccgtttcacc cgggcgataa agatcgcgcg ggccaaacat
     6121 gaaaaactgt ttgctatagc ctggcgcgcc agcaatgtta aattctgcta agtccagcgc
     6181 cggaagtttt aaatcgagta gcgttgtctg accgtcttta cgtgccagca ataatgcggc
     6241 gtttttatca ttttccagct gcacatgccc ctgagcgtca ctggttgcct gagtcagagt
     6301 ctgccctttc tcatttaata aagagacttc aattccttgc tgggccgcgc cgttttccag
     6361 actttgggta aagatatcca gacgattgtg ataacggtga gctgaaacgc cgatatcact
     6421 taacgtaaac agcgtcgcgg gattactgta atcgtaacgt ccagcctgat tcatcacagc
     6481 cagatacacg cccgcctgct gaagcggttt gatatcgccc agcggcagca ataatttttc
     6541 acgggtgtta cgcgcaggat tgagatcaaa ccgtccggtg tagaccagat ccgccatctg
     6601 cagcagtttg tctgactgcc agttcgccag cgaattgcgg tattcccatt ggctaatgaa
     6661 tgctggcaga gattctggct taacgcggaa gaagttaaca tcgacattat taacgttgag
     6721 cgccattacc ggcagccctt caacgacttt accaggcagc agcgaaccac ggctggcaaa
     6781 accgacactg ggttggatgt cgcgggtagt tatagttttt tcgtaatctt tactgaaggt
     6841 tgcgttgttg agtgctttga cctccttgcc aattgtaacg atcaaatcac gtttcggttc
     6901 gaggtggcgt aaacgcagct ctttaagatt atctgatagc tcccaggcac catccacttt
     6961 gccgcttttt ttatcgacga catgaataac gcgtgagaaa tcctgatccg ggtcgagagg
     7021 gatggagaac gtcagcacca gcgtggctgc accatcaagt tggacttcag agagatccag
     7081 caatgtcagc gccttaccgg cactctgttg ggccagtttt tgtcgctctg gcgcggagag
     7141 ttttgctgaa ctcgcgtttt ctgaagaggc cgctttagta acttcagaag gcgcatcttt
     7201 tttcaccgct gttggcgcgt tatcgttgtt gtcgcaccct gccagcgcca gcattagcat
     7261 gcaggcggct acgcgtaact ttttcatttt tcatccctgc cacaatggcc cgttagcaac
     7321 gtctaataat tattatgcgt gagaatttac gttatgtaat tcagtatcac gcccatacaa
     7381 tccgaatttt agtgagaatt gtggctcaga cagccaaatc agcctctttt gcttgtcgct
     7441 tttcactaaa catccgacaa tttagcctaa ccccggcaaa aatggagatg cctatgtcca
     7501 cgacatggtt tgtaggagcc gactggctcg ccgaacatat tgatgacccg gaaattcaga
     7561 ttatcgatgc ccgcatggcg tcgcctggac aggaagatcg taacgttgct caggagtatc
     7621 tgaatggaca tattcccggc gcagtgtttt ttgatatcga agcgctttct gatcacactt
     7681 ccccgcttcc gcacatgctg ccgcgcccgg aaacgttcgc cgtggcgatg cgtgaattag
     7741 gcgttaacca ggataagcac ctgattgtct atgacgaagg taatcttttc tcagccccac
     7801 gagcatggtg gatgctgcgc acctttggtg tagagaaagt gtcgattctg gggggtggac
     7861 ttgcaggctg gcagcgcgat gatctgttgt tagaagaagg tgcagtagag ctgccggaag
     7921 gagagtttaa cgccgcgttt aatcctgaag cagtggtgaa agtaaccgat gtgttattgg
     7981 caagccatga aaatacggcg caaattattg atgcccgccc ggctgcacgt tttaacgcag
     8041 aagttgatga acctcgccca ggtttacgtc gcggacatat tcccggtgca ttgaacgttc
     8101 cgtggacgga actggtgcgc gaaggcgaac taaaaacgac cgatgaactg gatgcgatat
     8161 tttttggtcg cggcgtcagc tatgacaaac caattatcgt cagctgcggc tctggtgtaa
     8221 cagcagccgt ggttttgttg gcactcgcga cgctggatgt gccaaacgtg aaactgtacg
     8281 acggcgcatg gagtgaatgg ggcgcgcggg cagatttacc ggttgagcca ttgaaataag
     8341 ctttttacag gcaataaaaa accgccgaat ttggcggttt tttattgctg gtctggttcg
     8401 cggcctttcc agcaggttga cttgtgttgc cggagcaacg caggcacccc acagtgttga
     8461 aatactctct tttatgcctg gctgtgtcaa gataataaac agagtaatta ttcaaacatg
     8521 agagtattat tcttaatggc ccttttcgcc gtctcgcaaa cgggcgctgg ctttaggaaa
     8581 gaatgttcca tgaccgtaaa tacaggcacc ccacagtgct tgctatcgcg gcaatatcgc
     8641 cagtggtgct gtcgtgatgc ggtcttcgca tggaccgcac aatgaagata cggtgcttct
     8701 gtatcgtact tattgtttct ggtgcgctgt taaccgaggt aaataataac cggagtctct
     8761 ccggcgacaa tttactggtg attaacaacc ttcagagcag caggtaagcc cgaatgccgc
     8821 cctttggggc ggcatatttt agattatccg attctgttta aagtcacgca aaaaaccacc
     8881 ccagcgacgt tcatagaatg gcgcaatatg ttcggtaata aagtggctaa ttcctttttc
     8941 cccttttttc acctgacaaa tatcgattgg ttcatcgcca ggtaatgtat cggtcgctac
     9001 acttcccgcc acctgaataa tttcttcgat atcaccatcg gcttcaatgc caataagtaa
     9061 attaggctgt gcctcttcgt tctctttaat tgaacaaata aaagcacgct tcacaggctt
     9121 aatggtttta aataaggtgg tgagtgaatc aatcatttgt gctggcggct ctgcgacttc
     9181 cgataatatc agcgattcac cgccttccag gatttcctgg ctgctcagcg gatttccctc
     9241 ttcaccaatc aacaaactga tttcacgcgg cataaattct ttaccggtcg gcagtttggc
     9301 attaaggaag agcgtttcgc caagggtcat ctcaaacaat gtgcgaacgg gcattacgac
     9361 aaatgcctgt tcgtcttcaa ccgcctgttg aagtgcttct aacgaggtaa aaaaaggaat
     9421 gacgctggtg ccgtcttctt tttcccagtg ctgtaaatca agcgcgctat cttcaaccac
     9481 agcctcgccc tgcgccgccg taccaggcac ccagacggtg gattccagta gagtacggaa
     9541 aaaggccggg cggtgcgccg gttcggttgc tgctttttcc agcaggtctt caagttcatt
     9601 ttttgtttcg gacataagaa ccacaattca ttcaacgttc ggggcacaag tgcctgatgc
     9661 gccatactta tcaggcctac aagggattca caatttgttg aatttgcaat actttgtagg
     9721 ccggataagg cgtttacgcc gcatccggca tattagttta cgccgttaac agattagcaa
     9781 tcgtgcgcac accaagtccc gtagcacccg cagaccactg ttcaaccggc gctttacggt
     9841 aagtcgccga gcagtcgata tgcagccagc cttgctgata gttctcaaca aagtgcgaca
     9901 ggaagcccgc tgccgtgctc gcgcctgccg gatacgccgc gcttccggta ttgttcagtt
     9961 cggcaaagtt agacggcagc tggctgcggt ggaattccgc cagcggcaga cgccagaatg
    10021 gttcgttctc ttgtgaggca ctcgccagca aacgaccggc aagcgcatcg tcaaaactga
    10081 acagcgcgtg ataatcatta cccagcgcag ttttcgccgc cccggtgagg gtcgccgcat
    10141 caatgatcat ttccggtttc tgcgcactgg catcaatcag accatcggca agcaccaggc
    10201 gcccttcggc atcagtgttc atcacttcaa cttttttacc gttgcgataa gtgatgatat
    10261 cgcccagctt gaacgcattg ccgctaatca ggttatccgc acagcagagg aacagcttca
    10321 cgcgcttgtt cagtccgcgc gtaatggcaa atgccagcgc cccggtaacc gttgccgcgc
    10381 cgcccatgtc cgacttcatc gagtccataa acgcagtctg tttgatgctg tagccgccgg
    10441 agtcaaaagt gataccttta cctaccaggc acgcgtacac tggcgcttct ttatcgccag
    10501 ttgggttgta atccagcgcc agcaataccg gagaacgttc tgaaccgcgt ccgacggtgt
    10561 gcagccccat ataaccttgc tcacgcagat cttcgccttt ggtgatccga taagtcacac
    10621 gatcgctcgc gacgttgctg atcagatcaa cagcacgctg tgccagttgc gatggtccca
    10681 attcttctgc cggtgcgttg atggtgtcac gcacccagtc gatgatcatc aggcggttat
    10741 ccagttcctg gcgctgggca tcgtccagat ccggccactc cactttacgc gtgcctttcg
    10801 gggctttgta accttgccag aatgcccagc atcgatccgc atcccagcct tcaccgctta
    10861 actgaacatg cttgatgccc agaccgtcaa tcttgcgcgc cgcacgctgg atcagcccca
    10921 gatcgtctgc cccgttcaaa tgcagggtaa tgccgtcatt attaatgctg taagttgctt
    10981 tttctcccca gcgcgcgtcg gcaggttggg tagagagggt aatcttcatc gcttctgtca
    11041 ttttagttat ccttctttgt aaaagggccg ccagttggca gccccaggat ttttccgcta
    11101 aatgattcgc gttgcaggaa ggcggcaagt gagtgaagcc ccaggagcat agataactat
    11161 gtgactgggg tgaacgagcg cagccaacgc atctgcggcg tgaagcatga cgcggaaatt
    11221 attcggcctc gtccagccag actaacaaaa tcgcttcgag gattttctcg ttggatgcct
    11281 gcgggtcgtc gtcgaaatct tccagatcgc aaatccactg atgcatatcg gtgaatcgaa
    11341 ccgttttcgg atcaagatcg ggatacgcat cgtacagtgc ttcgccaatt tcgcggctat
    11401 cggtccactt aagtcccata ctaacctctg ttaatgctca cgcgcatggt tgatcgtgta
    11461 acgcgggatt tcgaccacta aatcttcgtc ggtgacgcgc gcctggcagc ttaaacggct
    11521 ttccggctcc agtccccagg ctttgtccag catgtcgtct tcctgctctg agctttctgg
    11581 aagcgagtca aaaccttcac gaacgatgca gtggcaggtg gtgcaagcac aggatttttc
    11641 acaggcgtgt tcaatctcga taccgttacg cagagctgcg tcgagaatgg tttcaccgct
    11701 attagcttcc agaacagcgc catcagggca gagatcctga tgaggcaaaa taacaatctt
    11761 tggcatatta aacctcgtcc acggaatggc ctttcagcgc acgacgaacc gactggtcca
    11821 tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt tttaatcgct tgttcgatgg
    11881 cgtcaacgtc atcgccctgc gccacttcac tcaggtgagc ggcagcatcg tcaatgacct
    11941 gacgttctgc ggcgcttaac agcgcggcat cagcagccag cgcgccgtgc agactttcca
    12001 gcacacgcgc cgcttctact ttttgttctg ccagcattcg ggcttttacg tcctgctcgg
    12061 catagctcat tgagtctttg atcatcgaag cgatttcgct atcggtcaga ccgtaagacg
    12121 gtttgacctg aatagacgcc tcaacgccgg tggatttctc catcgccgtc acgctcaaaa
    12181 gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc accgccagcc ggtagcgccg
    12241 gaataccacg cagcgcaaaa cgcgccagtg agcggcagtc ctgcaccagt tcgcgctcac
    12301 cctgcattac atggatagac atcgccgtct gaccatcttt aaaggtggtg aaatcctgag
    12361 cgcgggccac tggaatagtg gtattacgcg gaatcacttt ctccaccagg ccgcccatcg
    12421 tttcgaggcc cagcgacagt gggatcacat caagcaacag catttcgctg tctggcttgt
    12481 tacccaccag aatatccgcc tgaatcgccg cgccaatagc gacgacttta tccgggtcga
    12541 tggaagtcag cggtggacga ccgaaaaatt cgcctacccg ttcacgcacc agcggcacgc
    12601 gagtagaacc gcccaccatc accacttcca gcacttcatc agcttctaca cccgcgtctt
    12661 tcagcgcgcg acgacaagcc aataaggttc gttttaccag tggcgcgatc agttcattga
    12721 attgttcacg gctgatttcg ccctgccagc ccgcaacgtt aacggtcacg gagtccgcat
    12781 cgctcagcgc gattttggct gcaatggcgg catccagcag ttcacgctga acgcggttat
    12841 cgctacgatc aggaatgccc gcctgctcgc gaatgtaatc cgccagcaga tggtcgaaat
    12901 catcgccgcc gagcgcggaa tcaccgccgg ttgccagcac ttcaaacacg ccgcgactta
    12961 agcgcagaat ggaaatatca aacgtcccgc caccgaggtc ataaacggcg atcacgcctt
    13021 cctgaccgga atccagcccg taggcgatag ccgcagcggt cggttcgtta agtaagcgca
    13081 ggacgtgaag gcccgccaga cgcgccgcgt ctttggtgcc ctgacgctgg gcatcgtcaa
    13141 agtacgccgg aacggtgata actacaccat ccagctcgcc tgccagggct tcagttgccc
    13201 gcgccgccag tgctttgagg atgtccgcag aaacgcgcac cgggttcagc agccccgccg
    13261 ccgtttcaat catcggcagg ccgttttcgc tggcctggaa ttgataaggc agatgcggat
    13321 agcgttgctg gatatcagcc agcgagcgtc ccatcaggcg tttaacagaa ctaattgtgt
    13381 tggcggtatc gagcgctgca ttagtacgcg cgtcataacc caccgaatgc ccttgctgtt
    13441 gatagtgaac aacagatggc agcaggtgac ggccttcatg gtcggctaac gtttcggcct
    13501 gaccgctgcg cactgtcgcc accagcgagt tggttgtgcc caggtcaata ccggccgcca
    13561 gacgacgctg atgcggcgca gcactcaaac caggttcact aatttgtaat aaggccatgt
    13621 ttagcttcca gaaattaaaa atcgagcagt ttttcttcga gttgttcggc actgcttcgc
    13681 agtttatcga gaaaacgcag cttacgcacg gtatccgccg ccgcgtccca ggtctcgttg
    13741 tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca tctttttcac acgtttgata
    13801 aagctttcca gccgcgcttc atctttcgcc tgttcgatct cgtccagctc ttcgcgcagc
    13861 tccaactgtt ccatcaggaa cgcggtgtcg cgcacagtat gctgctcgct ggcgagatca
    13921 aagccgtgca aagaaagcaa atattccgcg cgcattaacg gatgacgcag cgtttgccag
    13981 gcctgattaa tggttgcaga ttgctgtacg gcggcgagtt gttccgcctg gcttccgctg
    14041 gcgaatttat caggatgata ctgacgttgt agatcctgaa aacgcaggct cagcgcctgg
    14101 gtatcgagtt gataacgggc aggcaagcca aagagggtga agtaatccat aacattctca
    14161 gggctgcgat ccgccgcgat aacggcgaat caggtaaaac aacccccacg cgcaggcgac
    14221 cacggtgggg ttatcggtat gcgcatcaaa cgtggaagct ttcgccgcaa ccacactcat
    14281 ctttaacgtt cgggttggtg aatttaaacc cttcgttcag gccttctttt acgaagtcca
    14341 gctgcgtacc gtccagaaat tgcaggcttt tgccatcgac cacgactttc acgcctttgt
    14401 cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa ttccagtaca taagccatac
    14461 ctgaacatcc ggaggttctc acgcccagac gtaggccaaa ccctttgcca cggttagcca
    14521 gaaaggtatt tactcgcgct gctgcactgt cgctaagtgt aatcgacata accaaacctc
    14581 aactcttatt ttgcttcacg tttgctttta tagtccgcaa tggcggcttt gatcgcgtct
    14641 tctgccagaa tagaacagtg aattttcacc ggcggcagtt caagttcttc agcaatatcg
    14701 gtgtttttga tagcctgcgc ttcgtcgaga gacttccctt tcacccattc ggtgaccagg
    14761 gagctggaag cgatagcaga accgcagccg taagttttaa aacgcgcgtc ttcaatgata
    14821 ccttcatcgt tgactttaat ctgcaacttc attacgtcgc cacaggccgg tgcacccacc
    14881 atgccgctgc cgacgttctc gtcgttgttg tcaaaggaac ccacgttacg cggattctcg
    14941 taatggtcga taactttttc gctgtaagcc attataaatt ctcctgattc cgataccgat
    15001 taatgatgag cccattcgat gctgttcaga tccacgccct gcttgtacat ttcccacagc
    15061 ggagaaaggt cacgcagacg accgatggat ttacgaacta actcgatggt gtagtcgatc
    15121 tcttcttcag tagtaaaacg acctaaagag aaacggatag agctatgtgc cagctcgtcg
    15181 ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc ttgctgacgt acaggcggaa
    15241 ccggaagaaa ccgcgagatc tttcagcgcc ataatcagcg actcaccttc aacgtagttg
    15301 aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt caccgttcag gtaaacttct
    15361 tcgatatctt tgatgccgtt ccacagacgg ttacgcaggc cgcgcagacg ttccatctcg
    15421 gtcgccatct cttcttttgc gatgcgatag gcttcgccca ttccgacgat ctggtgaaca
    15481 ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc cgtgcatttg cgcttcgatg
    15541 cgtacgcgcg gtttacgacg cacatacagc gcaccgatac ctttcgggcc atagattttg
    15601 tgaccggaga aagacatcag gtcaactttc aactggctca ggtcgatagg cagtttgccc
    15661 acgctctggg ttgcatcaac gtgatagata atgccacgag cacggcacat ttcgccgata
    15721 gccgcgatat cctgcaccac gccgatttcg ttatttacgt gcatgataga aacaaggatg
    15781 gtgtcgtcac gcatcgctgc ttcgagttct ttcaggtcga taataccgtt acgctgcggt
    15841 gcgaggtagg tgacttcgaa accttcgcgc tccagctgac ggcaggtatc caataccgct
    15901 ttgtgttcgg ttttgctggt gattatgtgc ttgccttttt tctgataaaa gttggctgca
    15961 cctttgatcg ccaggttgtc agactcggtt gcaccagagg taaagacgat ttcacgcgga
    16021 tcagcgccga ccagatcggc aatctgatta cgggcgatat ctaccgcttc ttcagcctgc
    16081 cagccgaaac ggtgagaacg ggaggccggg ttaccaaagg ttccgtccat cgtcataaac
    16141 tgcatcattt tctcggcaac acgcgggtcc accggcgtgg ttgcggagta gtcgagataa
    16201 atcggtaatt tcattgctct ataaactccg tacatcactc aatgcaagga accaggctac
    16261 cggctggatg tacgaccgtg tttacggagc gcgatacgcc ccggcctgat tctgaatact
    16321 ttttattaag cgcgtaactt aacgtcgatc gcgtcttgtg tgcgggtgcg tggcgcgtcg
    16381 tgagtatgct gacgaccaga cacatccagc acttcctggt tgttaaccag ttcgcctaaa
    16441 gtaatgttat tgagaaaacc ggtaagacgg tcgctcaaat cacgccacag cgcgtgggtc
    16501 aggcatttat cgccgccctg gcagccgcct ttaccctgac aacgggtggc atctacagat
    16561 tcgtcaacgg cgctaatcac ttcgccaacg gcgatgctgc tggcatcttt gcctaacaga
    16621 taaccaccgc ctggtccacg tacgctggaa accagaccat ttttacgcag acgagaaaac
    16681 agttgttcca gataagaaag ggaaattccc tgacgttcgg aaatatcagc caacggtacc
    16741 gggcccgctt cagagttgag cgcaacgtca agcattgcgg tcacggcata gcgcccttta
    16801 gatgtcagtc tcatgtctta cttcacctca aactcgcccc tgcccggggt tttttattgt
    16861 aaaagtgggg gtattgcata gcagggtcaa gtctgacatt cccgagtaaa ttggtcaact
    16921 atttacttga ctgatttagt cgggtattta accttcagtg ccattttttt atcgtggcgt
    16981 ttgtacctgt tatccttcca gaccatgggc gggttgaggg caattattcg cattagcgca
    17041 ggcctgttcc cctcacccta accctctccc cgaaggggcg aggggaccgt ccgtgcactt
    17101 atctggctta gccctcaggc cttattacct ttattctgct gctctataga agccagaatc
    17161 ccgcgcagga tattcaattc ctggctttcc gggcgcgcac gggtaaacag acggcgcaat
    17221 ttattcatca cctgccccgg atggttttca cggataaaac cggttgccag cagcgtttgt
    17281 tccagatgac cataaaaacg ctccagatca tcgaccagcg gatacggcgt ctcttcatgc
    17341 tcgacctgct cgccattttc ctgagttgcc agccaggcca tacgcacttc ataggcaata
    17401 acctgaaccg ccatcgccag gttcagcgag ctgtattcag ggttagcggc aatcgcgaca
    17461 tgataatgac acttctgcaa ctcttcattg gtcaggccga cacgttcgcg accaaacacc
    17521 agcgccaccg gggtatttgc cgcttcagca acgcttttca gaccgcattc gcgcgggtcg
    17581 agcatcggcc acggcagcgt gcgggaacgt gcgctggtgc ccaccaccag gctacaaccg
    17641 gctaacgctt cgtcgagcgt atcgacgatg tgagcattac caatcacatc gctggccccc
    17701 gccgccaggg caatcgcctg ggagtcaggt ttcaccagtg gattaaccag ccacagattg
    17761 gttaatccca ttgttttcat ggcacgggca acagaaccca tattgccggt gtgtgacgtc
    17821 tccaccagca caattcgaat attttgcagc attgtctttc ttcgtctaaa gattattcgc
    17881 gcatcttatc ataaaacgaa gacagatgcc gatctcgctg ctatactctg cgccgttttc
    17941 ccgttcttta acatccagtg agagagaccg atgcatccga tgctgaacat cgccgtgcgc
    18001 gcagcgcgca aggcgggtaa tttgattgcc aaaaactatg aaaccccgga cgctgtagaa
    18061 gcgagccaga aaggcagtaa cgacttcgtg accaacgtag ataaagctgc cgaagcggtg
    18121 attatcgaca cgattcgtaa atcttaccca cagcacacca tcatcaccga agaaagcggt
    18181 gaacttgaag gtactgatca ggatgttcaa tgggttatcg atccactgga tggcactacc
    18241 aactttatca aacgtctgcc gcacttcgcg gtatctatcg ccgttcgtat caaaggccgc
    18301 accgaagttg ctgtggtata cgatcctatg cgtaacgaac tgttcaccgc cactcgcggt
    18361 cagggcgcac agctgaacgg ctaccgtctg cgcggcagca ccgctcgcga tctcgacggt
    18421 actattctgg cgaccggctt ccccttcaaa gcaaaacagt acgccactac ctacatcaac
    18481 atcgtcggca aactgttcaa cgaatgtgca gacttccgtc gtaccggttc tgcggcgctg
    18541 gatctggctt acgtcgctgc gggtcgtgtt gacggtttct ttgaaatcgg tctgcgcccg
    18601 tgggacttcg ctgcaggcga gctgcttgtt cgtgaagcgg gcggcatcgt cagcgacttc
    18661 accggtggtc ataactatat gctgaccggt aacatcgttg ctggtaaccc gcgtgttgtt
    18721 aaagccatgc tggcgaacat gcgtgacgag ttaagcgacg ctctgaagcg ttaatgactc
    18781 aggcgggtga tatcactcac ccgccctcgc ctttcaggcg ctattccgaa atacttcctc
    18841 accgctttac tttctttcgt cactctccca ccatctttcc ccgattaatg gatgataaag
    18901 aagtcgcaag gaaaggttat ggcactgcca gtgaacaaac gcgttcccaa aattctgttt
    18961 attctctttg ttgttgcctt ctgcgtttat ttagtgccgc gcgttgccat caacttcttc
    19021 tattatcctg acgataaaat ttacggtccc gacccctggt cggcggaatc cgtcgaattt
    19081 acggctaagg acggtactcg tctgcaaggc tggtttattc cttcttcgac gggccctgct
    19141 gacaacgcca tcgcaaccat cattcatgct cacggcaatg ccggaaatat gtccgcccac
    19201 tggccgctgg tcagttggtt acccgagcgt aatttcaacg tttttatgtt tgattatcgc
    19261 gggtttggta aatcaaaagg cacgccgtcc caggccggat tgctggacga tacgcaaagc
    19321 gccatcaatg tggtgcgcca tcgcagtgat gtaaacccac aacggctggt gctgttcggg
    19381 cagagtattg gcggggcgaa tattcttgct gttattggtc agggtgatcg tgaaggcata
    19441 cgtgcagtga tcctcgactc cacatttgcc tcttatgcaa ccatcgccaa ccagatgatc
    19501 cccggcagtg gctacttact tgatgagagt tacagcggcg aaaattatat cgccagcgtc
    19561 agcccgatcc cgcttttact cattcacggt aaagctgatc acgttatccc atggcagcac
    19621 agcgaaaagt tgtatagcct ggcaaaagag ccaaaacggt tgatcctaat cccggatggc
    19681 gaacacattg atgctttttc cgatcgtcac ggcgacgttt atcgcgaaca gatggtgaac
    19741 tttatcctta gcgcgttgaa tccgcagaac taacccatga tcgctagcac gataatcatt
    19801 cacaaaatca ccttaagaca tgctaatcca ctggtcagga cagtttaaga tgagaaaaat
    19861 tctgtgacgc ttgccaacat ttctgatgat tagcattccc ttcgccattt ccctgagcaa
    19921 actttagcta ttcttatcaa ttatgcttat gggagatcta cagatgatgc ctacgcttgc
    19981 tccaccatct gtcctttcgg ctccccagcg ccgctgccag atcttgctga cgctctttca
    20041 gccggggttg accgccacca tggcaacctt cagcgagctt aatggtgtgg atgatgatat
    20101 tgccagtctt gatatcagcg aaacaggacg ggagatcctg cgctatcatc aactcacact
    20161 gacgactggt tatgacggta gctaccgggt tgaaggtaca gtgcttaacc aacgtttgtg
    20221 tttatttcac tggctacgac gtggtttccg tctgtgtccg tcatttatta ccagccaatt
    20281 cacccccgcc ctgaagagtg aactgaagcg gcgcggaatt gcgcgtaact tttacgacga
    20341 taccaatcta caagcgttag tgaatctctg ctcccgacgg ctgcaaaaac gctttgaatc
    20401 gcgcgatatt catttcctgt gtctgtatct gcaatattgt ttgctgcagc accacgctgg
    20461 aattacgcct cagtttaatc cgctccaacg tcgctgggcg gaatcctgcc ttgaatttca
    20521 ggtagcgcag gaaattggac gccactggca gcgtcgggcg ctccagcctg taccacctga
    20581 tgagccactg tttatggcac tacttttttc catgttgcgg gttcccgatc cattgcggga
    20641 tgcgcatcag cgggacagac aattgcgtca gtctatcaaa cgtctggtaa accattttcg
    20701 tgagctggga aatgttcgtt tttatgatga acaggggtta tgtgatcagc tttataccca
    20761 cctcgcccag gcgttaaatc gcagtttgtt tgccatcggt attgataata ccctgccgga
    20821 agagttcgcc agactgtatc cacgcctggt acgcaccacc cgcgcggcgc tggccggatt
    20881 tgaaagtgaa tacggcgtcc atctttctga tgaggaaagt ggtctggtcg cggtgatttt
    20941 cggtgcctgg ctaatgcagg aaaacgatct gcatgaaaaa cagattattc tactgaccgg
    21001 gaatgatagc gagcgagaag cgcagattga gcaacagtta cgcgaactaa cgttactgcc
    21061 gctcaacatt aaacatatgt cggtaaaggc atttttgcag acaggcgctc cgcgtggcgc
    21121 ggcactgatt attgctcctt ataccatgcc gttaccgctc ttttcaccac cgctgatcta
    21181 tacggacctg acactgacaa cacatcaaca ggagcagatc cgcaaaatgc tcgaatcagc
    21241 atgagggaac aactttcggg cgcaaaaaca ttgccggaag cgccaccagc gccatcaccc
    21301 agaacacgcc gtggcccaga tattgataca ggaaaccggc gaaaacggtc atgatagcga
    21361 tactgccgcc catcgcgacg gcagagtaaa ccgcctgtaa acggatgact tcgctaccct
    21421 ggcgagcagc aatatagcgc atggcggcca ggtggcagac cgtgaaggtg ccgcaatgca
    21481 gaatttgcac cactatcaac cacggcaacg ccgtagttgc tcccataatg ccccagcgca
    21541 ctacgccgca aatcgctgag atcaacagca tatcgcgtgc actacaacgg cggaaaagtt
    21601 tattactcag tgcaaagata atgacttccg ccaccacgcc cagcgaccac aaatacccca
    21661 ccgccgaggc cgagtagcca gctgcctgcc agtaaatggc gctaaaaccg taataggccg
    21721 catgtgcccc ctgcaataaa caaacgcagg ccagaaagcg ccagttctgg cgaaccagcg
    21781 ccaaccacgc tgaccaaccg gtgctctcct gctggcggct tgccccttgt ggctgaatcg
    21841 tcggacggat gagaaagccg agcagcatgg atgccacgcc caacgtcaac agcacgagga
    21901 tcacccgata atcaaacata gtgaccagtt tgcccgtcag cgccgagcca atgacaaacg
    21961 ccaccgagcc ccacagtcgc actttgccgt aatcaagcgg gaactgcttt tgccacgtat
    22021 tcgccagtgc atcggtcaac ggtaccagcg gtgagaaaaa gaggttaaag ccaatcatca
    22081 ccagcatcag ccacgctacg tgcgcccccg cccagaaggc gacagcaaag agaagtgtca
    22141 gcagtgccag cacgcgcaag gcggaaatca ggcgagaagg atcgctgacg cggggcgcga
    22201 tgagcaaact cccgagaaaa cgggcaacca gacctgcccc caataacagg ccgatggttt
    22261 ctggcgttaa accaatccct ttaagccaga cgctccagaa aggtagaaaa atgccgtaac
    22321 taaaaaagta tgtgaaatag ccgagcgcca accagcgcgt ggattgcaaa accatgagtc
    22381 cctcccgtca ggaggcgtta gtctggagtt aatctaatgt tggtgcaagt tgaaaaataa
    22441 gaatattcga gcctgcttcc cgatgtctgt aacgccagtt cacaagcact caaaaactgc
    22501 gatccgtgac gcatgttgca aagataatgc gtaattatgc cgttacgctt gccaaacgtt
    22561 cctgcacaat ggcgatgaaa tcgcgtaatg ccggcttcat ctcccctttc ttccacgcca
    22621 tcagtaaagc aatggaaggc acatttccgg caatgggacg aaaaacaacc tgcccggtat
    22681 taaaattatt catataaccg ggtatcaaag tgacgcccag ccccatgccc accagattca
    22741 tggtcaccag aatattcgtt gccacctgga cgatatttgg ctggctgttt tcttgtgcaa
    22801 accacgcttt aacgatcggc gcaagcgaac cggaatacgc cggatcggta ctgacgaaat
    22861 tcacgccatc cagttgcgcg gcggtgatct ctttttcatg tgctaaaggg tgatcaaccg
    22921 gtaacaccac cactaatggt tcgtcaaaaa gctccagata atcaatctcc gggctataaa
    22981 caggatggcg cattaagccg acgtcgagtt caccacggcg aattttttcc tcctgttgcg
    23041 tggtgattaa actcaccagc tcaatcaagg tgtctggctg tctgagacga aacatcggta
    23101 atacttttgg cagtaaattc acttccgccg atggcacaaa gccaatggtt aattgtctgt
    23161 cttcctgaac aattttccgc gcccgtaatt tggcattttc cgcttgttcg aggattgcca
    23221 gcgcgtcctg gagaaaacat tctcccgccg ccgtcaacgc gactttgcgc ttatccctca
    23281 ccagtaacgg aacaccgaca cagttttcaa gatcgcggat ctggctgctt aacgaaggct
    23341 gtgaggtatg cagtttttcc gccgcacggg taaagttcag tgcctgcgcc actgcgacga
    23401 aatagcgtaa atgccgtagt tccatcacct tccccttgtt atcgaaaaaa cgtctcagct
    23461 ggtagaaatt aactatttca catattagca accaaccagc aacatcctta tgacacaaaa
    23521 atagaaggtc aatacatctt atctttcagg attaaaaaat atgaccacac cctcagattt
    23581 gaacatttac caactgattg acacccaaaa cggtcgggtc actccgcgta tttataccga
    23641 cccggacatt taccaactgg agcttgagcg tattttcggt cgttgctggc tatttctcgc
    23701 ccacgaaagc cagatcccaa aacccggtga tttctttaac acctacatgg gagaagatgc
    23761 agttgtcgtg gtgcgccaga aagacggcag catcaaggcg tttctcaacc aatgccgcca
    23821 ccgggccatg cgtgtgagtt atgcagattg cggcaacact cgcgccttta cctgcccgta
    23881 tcacggctgg tcttatggca ttaacggcga gttgatcgat gtaccgctgg aacctcgcgc
    23941 ctacccacaa gggttgtgta aatcccactg ggggctaaac gaagttcctt gtgtggagag
    24001 ttataaaggg ctgatttttg gcaactggga taccagcgca ccgggcctgc gtgattacct
    24061 gggtgacatt gcctggtatc tggatggcat gctggatcgt cgcgaaggcg gcaccgaaat
    24121 tgtcggcggc gtacaaaagt gggtgatcaa ctgcaactgg aaattcccgg cagagcagtt
    24181 cgccagtgac cagtatcatg ctctgttcag ccatgcttct gccgttcagg tattaggggc
    24241 gaaagatgat ggcagcgata agcgcctcgg tgatggacaa accgcccgcc cggtgtggga
    24301 aaccgccaaa gatgccctgc aatttggtca ggatggtcac ggcagcggct tcttctttac
    24361 tgaaaaacca gatgctaatg tctgggtcga tggcgcagtt tccagctatt accgcgaaac
    24421 ctatgccgaa gctgaacaac gtttaggtga agttcgcgcc ctgcgcctgg cgggtcataa
    24481 caatattttc ccgacacttt cctggctcaa cggcactgcc acactccgcg tctggcatcc
    24541 gcgcggccct gatcaagttg aagtgtgggc gttctgtatt actgacaaag ccgcctccga
    24601 tgaagttaaa gccgcttttg aaaacagcgc cactcgtgct tttggtcctg ctggttttct
    24661 cgagcaggat gactcggaga actggtgtga aatccagaaa ttgcttaaag gccaccgcgc
    24721 ccgcaacagc aaactgtgtc tggaaatggg gcttggtcag gaaaagcgcc gcgacgacgg
    24781 cattcctggc attactaact atatcttttc agaaactgct gctcgtggaa tgtaccaacg
    24841 ctgggccgat cttctgagta gcgaaagctg gcaggaagtg ctcgataaaa ccgccgctta
    24901 ccagcaggag gtgatgaaat gagtgcgcaa gtttcactag agttacatca ccgcattagc
    24961 cagtttctct ttcacgaagc cagcttactg gacgactgga aatttcgtga ctggctggca
    25021 cagctcgacg aagagatttg ttacaccatg cgcaccacgg tgaacgcgca aacgcgcgac
    25081 cgccgcaaag gtgttcagcc gccgacaacc tggattttta atgacaccaa agaccagctg
    25141 gagcggcgaa tcgcccgtct ggaaacgggc atggcatggg cagaagagcc gccgtcacgc
    25201 acccgccact taatcagcaa ctgccagata agcgaaaccg acatcccaaa cgtatttgct
    25261 gttcgggtga attatctgct ttatcgggca caaaaagagc gcgatgaaac gttctatgtc
    25321 gggactcgtt ttgacaaagt tcgccgtctg gaagatgaca actggcgctt gctggaacgg
    25381 gatatcgtcc tggatcaagc ggtaatcact tcccataacc tgagtgtact gttctgatga
    25441 atcgaattta tgcgtgtccc gttgcggatg tgccggaggg tgaggctctc cggatcgata
    25501 cctcgcccgt catcgccttg ttcaacgttg gcggcgagtt ttatgccatt aacgatcgtt
    25561 gcagccatgg taatgcgtcg atgtcagaag ggtatctgga agatgacgcc acggtggagt
    25621 gcccgctaca cgccgccagt ttttgcctga aaacggggaa agcgttatgc ctgcccgcca
    25681 ccgatccgct caccacttat ccagtacacg ttgaaggtgg tgacattttc atcgacttac
    25741 cggaggcgca gccatgagcg atctgcataa cgagtccatt tttattaccg gcggcggatc
    25801 gggattaggg ctggcgctgg tcgagcgatt tatcgaagaa ggcgcgcagg ttgccacgct
    25861 ggaactgtcg gcggcgaaag tcgccagtct gcgtcagcga tttggtgaac atattctggc
    25921 ggtggaaggc aacgtgacct gttatgccga ttatcaacgc gcggtcgatc agatcctgac
    25981 tcgttccggc aagctggatt gttttatcgg caatgcgggc atctgggatc acaatgcctc
    26041 actggttaat actcccgcag agacgctcga aaccggcttc cacgagctgt ttaacgtcaa
    26101 cgtactcggt tacctgctgg gtgcaaaagc ctgcgctccg gcgttaatcg ccggtgaagg
    26161 cagcatgatt ttcacactat caaatgccgc ctggtatccc ggcggcggtg gcccgctgta
    26221 caccgccagt aaacatgccg caaccgggct tattcgccaa ctggcttatg aactggctcc
    26281 gaaagtacgg gtgaatggcg tcggcccttg tggtatggcc agcgacctgc gcggcccaca
    26341 ggcgctcggg caaagtgaaa cctcaataat gcagtctctg acgccggaga aaattgccgc
    26401 cattttaccg ctgcaatttt tcccgcaacc ggctgatttt acgggtccgt atgtgatgtt
    26461 ggcatcgcgg cgcaataatc gcgcattaag cggtgtgatg atcaacgctg atgcgggttt
    26521 agcaattcgc ggcattcgcc acgtagcggc tgggttggat ctttaaggaa gtacgatgaa
    26581 agaaaaaacg atcattattg tcgggggcgg gcaagcggcg gcaatggctg cggcctcgct
    26641 acgccagcaa gggttcaccg gtgagctgca tctgttttcc gatgagcaac atcttcctta
    26701 tgaacgccct ccgctctcga aatccatgtt gctggaagat tccccacagt tgcagtctgt
    26761 gttacccgct cactggtggc aggaaaacaa tgttcatctg cattccggtg taaccatcaa
    26821 aacattgggc cgcgacacac gagagttagt gttagccaac ggcgaaagct ggcactggga
    26881 tcagcttttt atagcaaccg gcgcggcagc tcgaccgctg ccgttgcttg atgcactggg
    26941 agaacgctgc tttaccctgc gccatgccgg tgatgccgcc agactgcgag aagttctgca
    27001 gcccgaacgg tcagtcgtga ttgtcggtgc cggaactatt ggtctggaac tggctgccag
    27061 cgccacgcag cgcagatgta aggtgacagt gattgaactg gcggcaaccg tcatgggccg
    27121 taatgcacca ccgcccgtgc aacgctatct tttacagcgc catcagcagg ctggtgtgcg
    27181 cattctgctc aataatgcca ttgaacatgt ggtcgatggt gaaaaagtag aactgacgct
    27241 gcaaagtggc gagacgcttc aggccgatgt ggtgatttac ggtattggta tcagcgccaa
    27301 cgaccaactg gctcgcgagg ccaaccttga tactaccaat ggcattgtca ttgatgaggc
    27361 ttgccgcacc tgcgatcccg cgatctttgc cggtggcgat gtggcaatca ctcgtcttga
    27421 taatggtgca ctacaccgct gcgaaagctg ggaaaacgcc aataaccatg cgcaaattgc
    27481 cgctgccgca atgttagggc taccgcttcc gctactgccg ccgccgtggt tctggagtga
    27541 tcagtacagt gataacttac agtttattgg cgatatgcgt ggcgatgact ggctttgtcg
    27601 tggcaacccg gaaacccaga aagcgatttg gtttaatctg caaaacggcg tgcttatcgg
    27661 tgcggtaacg ctgaatcagg gacgtgagat tcgctcaatc cgcaaatgga tccagagcgg
    27721 caaaacgttt gatgcgaaac agctgacaga tgagaacatc gcgcttaaat cactgtaacc
    27781 aggataatta gcgaatatct caacgcctgg ggcgtggcga ggtaccagag tgtgtattac
    27841 gtttaaatca cattatcttg caaagggatt ggttatgaac tcactacgtt attttgattt
    27901 tggtgctgcc cgccccgttt tgttattaat tgcccgtatc gccgtggtcc taattttcat
    27961 tatttttggt tttcccaaaa tgatgggctt tgacggtacg gtccaatata tggcctcgct
    28021 gggcgcaccg atgccgatgc tggcagcgat tattgcggta gttatggaag tgcccgccgc
    28081 gatattaatc gtgcttggct ttttcacccg tccgctggcg gtactgttta ttttctacac
    28141 gctgggtacg gcggtgattg gtcaccatta ctgggatatg accggcgatg ccgtcgggcc
    28201 aaatatgatt aatttctgga agaatgtcag tatcgctggc gcgttcttgc tattggcaat
    28261 taccgggccg ggggcaattt ctctcgatcg ccgttaggaa agatgccgga tgcggcgtga
    28321 acgccttatc cggcatttta taaattacag ccactctacc cgcaacgaca tctctgaggt
    28381 tgttgattcc cccggcgcaa gcgcaatgag gtcaccccct tccgggcgat gatggtcatc
    28441 cggcgcatgg ctcatcggct ccagacagaa gaaatcaaac gcatatcctt tatcaaacgc
    28501 cgggtcggaa acaaagatga aataacacgg tgcaggtggc gtcgtttcta tgatgatggc
    28561 ataaccctcc tgcggctgct cgatgcgggc ctgaccattc catccggcaa aaccattgtt
    28621 cacccactgg cgcggcaacg gcgctagctg gttaaaatcc agttcttgcg gtagctgctc
    28681 gcaaaactca cccgccagcc actgctcccg ctccagccag taaccgctcg cctgtgcctg
    28741 aatccgcgtt tgcggcgaca acggaaaata aggatgccag ccggtaccaa atggcagcgt
    28801 ctctgcccct tgattggtga cagagagcgt caccgtcagc gtatccgccg ttaaatgaaa
    28861 cgcctgactt acccgatagt gatagacacc gctgcgatgt tcatacacca gacacaaact
    28921 atcatcgctg tgcgagacac attgccactc gcccagccag ccatcgccgt gtagatagtg
    28981 cgcatcccac tcaacattcg gttgcagttg atactcacgc ccctgccaga caaaacggtt
    29041 gccgctcacc cggtttgcaa atggcaccag cggaaaacaa gaggcatcgg ttgccacacc
    29101 gcttttttta ccaggacgta agagcggcgt cgtatcgcgc cagaagcctt cgattacgcc
    29161 gccctggtca gaaacgccca gctttagcga cccgtgggat aaggtataga tggtcatccg
    29221 ctaactcctt aatccgggaa gttaatcaca actttcccgc atttgccgct cgccatcagc
    29281 gcataggcat cacctgcctg ttccagcgag aagcgatggg taatggcgtt acgcggccac
    29341 agcttccagt cagtcaaatc gtgggcgcat ttttccatat ggaacagact ggtcacccag
    29401 gagccaataa tccgccgttg atggtgcatc agatcggcgc tgacctcgaa ttccactttt
    29461 ccggtttcac caatgtaaac cacccgcccc cagtcagcgg tggattgcag tgccagtaag
    29521 cgacctgcgg cattaccgga acaatcaagc gcaacatccg cgccgccgtg agtgagttcg
    29581 gcgattatct gcggcagacc ttcagtggtc gctaaatagc cgtgatccat cactcctaac
    29641 tgttttgcca tcgccagacg ttccggcagc atatcaacgc cgataatccg ttttgcaccg
    29701 cgacctttcg ccagcatcat ccccatcatg ccgactggcc ccagaccgac caccagcacg
    29761 ttatcactgc cggaaacttc gccgcgcaaa atcccttcat aggccgtgcc aacgccgcaa
    29821 ctgataaacg caccatcttc gtagctcagc gcatccggca ggaggatcag atctttttct
    29881 tccgccagca agtattcggc atgaccgccg tcacgctgcc agccgtaagc cgcttttcct
    29941 tcgccagtac aagaaatagg aaaaccgcga cggcagttcg ggcaaaaacc acagccagaa
    30001 atgtgataca ccagcacgcg gtcgccctct ttaaaatggc ggcagccttg ccccatcgcc
    30061 acaatctgcc cgcacggttc atgaccgttg ataaatccct agtacaacgg tttatcggat
    30121 gccgccgccg tagcgcggtg ctggtggtag atatagtgga catcgcttcc gcaaattccg
    30181 gaggatttca ttttgatcag tacctggtta atccccggcg tcggcactgc aacttcccgc
    30241 agatcgacgg tcgaatttcc tggtaaataa gctgccagca tcgttttcat aaatccctca
    30301 ttaacaatac gattaatttt catccctgcc gcacccgcgc caggggcgtt aaggtttagc
    30361 gtttcgcttt actgctacgc tgggtcagca agatattcgc cagcaccgcc accacgatga
    30421 tgacgccgcg caccacctgc tggaaaaagg agttaatccc gagcagcacc agaccgttac
    30481 cgattagcgt aatcaccagt acgccaagca gcgtaccgaa caacgagccg cgaccaccgg
    30541 aaagcgccgt accgccgacc acgaccgcgg cgatgacgtc aaactccaga ccgtttgcgg
    30601 cacctgcgtt accggaaccg aggcgcgccg ccaacaaaat gccggtcacc gccgctaata
    30661 atcccgaaag ggtaaagata agaatgcgta cccgacgaac gttaataccg cacaactgcg
    30721 ccgccgtggc attaccgcca acggcaaaaa ccgagcgccc gaaggcggtt ttgcggctaa
    30781 tgaacacaaa cagcgcaaac agcaccatca tgatcagcgc ggataccggc acaccgagaa
    30841 attgtccgcc cagccagtcc agcacctcgt tttcgtcaat cggcactggc agagcgttgg
    30901 tcataaacaa gcccattccg cgcagggcgc tccacagccc cagcgtggcc acgaaacttg
    30961 gcacgttaaa cacgccacgc agcaccccgg cgagcgttcc catcagcgcg cctaacagca
    31021 acaccagcag acaagcaatc gccaacggaa cttcaaattg cagcaaaaat gccaggcata
    31081 ccgagacaaa agccaccatc ggcccaacgc tgacatcaat ttcaccggag ataataatca
    31141 gcgtcatcgc ccaggcggca atcccaatgg tggcggcatc gcgcagcacg ttcatctggt
    31201 tattcaatga gataaagcca ggcgcgttca gggagaagac cagataaaga atggcaatca
    31261 ccaccagcaa accgatctca ttaatatggt gactgacaaa ttgtttgagc gagacgctct
    31321 tgccctgcgg caatggtaat gacgaagcag acatcttcag gttcctcggt aaaattcatc
    31381 agtgcacaga cagaatggcg gacatcagct catccacatt gaccggagcg tgaaactcct
    31441 gcgagaacgt gccgtgctgt aataacagga tgcggtcaca caccaacggc aactcctcca
    31501 cttcactgga gataaacacc acgctttttc cttcggcagc cagctcacgg acaatacggt
    31561 aaatctgctg tttggcttcg atatcgacgc cgcgcgttgg ctcgtcgagc aacaaaatct
    31621 ggctggcggc atagacccaa cgaccgatca ctactttttg ctgattgccg ccagaaagcg
    31681 tgccgatggg cgtttcgcta ctggcggcct tgaccgtcat ccgctgcatc acttcttcgg
    31741 tcaggcggcg gatggtggac cattgcagca ctccgttggc gctgattttt tgccgattgg
    31801 tcagcactgt attttcgtca acgcccaacc agggaatgat ccccgcttct ttgcggtttt
    31861 ctggcgtata gccaatgccg cgtttcagca tgtcgccgta atcggggcgc gtgattttct
    31921 cgccgttgat aacaatttcg ccctgttcat acgtctccag cccaacaatc gccttcagca
    31981 attcactgcg ccctgccccc agcagaccag caatgccgag cacttcgcca cgacgtagcg
    32041 taaagctgat atcctccagc ttgggcttat ggcgtaacgc acggacttcc agcacggcct
    32101 gatccacaat ttcctgaggt gcaaccggcg caatatcaac gtgatcgcgc cctagcatta
    32161 gcgacacaat atgatgcgtg gaagtgtttt cgagcatcac atcgccagcc acctgcccgt
    32221 cgcgcataac ggtggcactg gaggcaatgc ggcgaatttc ttccatccgg tggctgacat
    32281 aaatcaccgc cacgcccagt gctgacatct ttttcaccgc tctgatcacc agttcaactt
    32341 ccgcactcgc aagcgaactg gtaggttcat caagaatgac cacgcgcggc tcgcccttca
    32401 tcacccgcgc aatttccacc agctgcttat gcgccgggct tagtgttgaa acaagttgtt
    32461 caggactaac gtcaacgccc agcgcctgta agcaacgttg ggcatcctgc gccatttgca
    32521 ggtaatcaat catgccgttg cggcggggcc actgaccgag gcagaggttt tccgccaccg
    32581 tcagcccttc caccagactt aactcctgat aaaccgcgcg tacccccagt tcagcggcac
    32641 ggcgagtcag cgtagcttcg tcaccttcca gtcgcgtctc gccaatccag atttcaccgc
    32701 tatccggacg ttcactgccg gtaagcattc gaatgagggt cgatttgccc gcgccgtttt
    32761 tgcctaacag cgcacgaacc tcgcctttat tgagcgtgaa gttaacgtta tccaacgcaa
    32821 cgacgccggg ataacgctta tttcctgcca ccacttttgc taccgggact gcctctgttg
    32881 ccgtgaacat agggacctct gcgaatcagc gattagggca gaccatcaac gtgcgttgcc
    32941 agccactgtt taccgtcttc cgttttggta tagagatcga taggcacctg aatcactttt
    33001 tcaccgtcgg cttgtttatt gataaccttc aatgtttgcg cgaaaacagc attgcccatt
    33061 ttcttaccgg aaatatccac taccgctttc agcacctgat tgttttccag ctcctgagca
    33121 atttcggttg tcatatccga accgaaaaca gcaatttttc cggcctgatt ttgattacgt
    33181 accgctttta ccgcaccgag tgtcgcaccg cccgattccc ccataatggc gttgagatcc
    33241 ggcgtggaga taatcagttt ttcaccaacg gaaatcgctt tatctaaaac agtcccttcc
    33301 tgattagcga caatttgcgc gccgggaacg cgggatttta atacttcttc aaatccttta
    33361 cgtcgctgca cacaaacttc aaaggcttcg caattgatga cggcaatttt cggctggtca
    33421 attttattgg caataaaata atcggcggca gcgttaccca gttttttacc aaattccagc
    33481 gggtcaccca ccagatacgc cgagacatat ttatcgaccc ccttttgatt aatacaggtg
    33541 ttgtagcaaa tcaccggaat acccgcttca ctggcgcgac gaacggtacg gctactgcca
    33601 ttttcagaca ctgccgataa aataatagca tcgacattac gcgccacgag ggtatcaaca
    33661 aaggtacttt ctttcgaaat atcaccctgg gcgttagttt caattaactg tacctgtact
    33721 gaagaatctt ttgccgcatc ctgaactccc tgacgcactc cagcgtaata tccctgagta
    33781 tcaaggtata ttgcgccaat ggtcatttct ttatccgcag ctctggcaaa tagtgcgcta
    33841 cctaatagcg tagccattaa taatagatta cgggttgttc tcattttttt aggcatagag
    33901 cctcctgtag ggtttttatt aacaacggct tattctaatt attttgtgat gagcggcagc
    33961 gcgtgccgct caaatattac agttaatagg gtttagtgaa cgtagttaaa aataaaaggc
    34021 atcacggtgg tgaataatgc cgctttcggc caggccggat taatcgccgt caattcagcg
    34081 cgagcagatt cgtattgcgc aatctcgcct aatcccgcgg ccgccagcat cgccaccatc
    34141 aggcattttt ctttatgctg ctgttgtaaa tcgccataca gcgacaacaa gtcaggctgc
    34201 gagacggcaa agaaatccgc ttcgatactg gttttcgcca tctcttgcgc ccactgtttc
    34261 atttcactaa acagttgctg tgcggtgtgt tgttcgccca gtaatcgcag cgccattcct
    34321 tgccagaaga ggtaatcaac cggctgatcg ttgtaatagc tgtggatgtt aatggtgcga
    34381 tcgccggtcg ccgccagatg taaacagcgc gtcgcttcag tttcatcgcc ctgggctttg
    34441 gcgcatatcg cctgccagaa ccagatgtcg ttatcagttt gccccggtaa acggccttcg
    34501 cttaaattct ccggataatg cagcgcggca tgaagcagtt cgctggcctg ctgcggctct
    34561 ctggcatcaa gatgctgcca ggcgcgtaat aactggttga ggataaactg actggtgacc
    34621 ttcccttccc cgccttccca cgggtggaat ttgcgcgtgg cgagaatgtc cgccgctttg
    34681 tctgcctgcc ccgtgagatg ccacaaattg agcagttctg cggtcatgtc gtcgcgtttc
    34741 agcgcaattt ccagattatt ttccagacgc gccagtcgtt tctccggtgt ggttccactt
    34801 agcttatcaa gcaaatcccg ttcgaaaagc agacgtgcat cctgcggcgc aagctgataa
    34861 gcattatcaa gataacgcgc ggccagctca taatcgtgtt gcttattcca cgcatggatc
    34921 gctaacccgc gccagccgtc ggcaaactcc ggcgacatct ctacgcaacg ttgccataag
    34981 gcaatggctt cgttgtagct acgtttgttg tagtagaagc aggccagtaa atggcgagca
    35041 aaccagcact cttcaatact ctccagcgcc gccacttctt ccagcgtatt cgggaaacgg
    35101 acaaactgcg ggaagacatc aatggctttt gcgaccagtt cgccacgttc ggctttcggc
    35161 agcaagctgg cttgcaggta aagcggcagc gtgcgctggc agtccagtgc attcagcatc
    35221 tctgccgcga gggtgggcat tccccagtta atcagttgcc cggcggtcat cagggcgtta
    35281 acgtcgcgtc cctgacacag accgcgccac tgcgcgagag ctgattcgct acgaccatcg
    35341 aaccagttca ggcaccacag agtggcgttc agcggataat cgcgcagcag tttctcgcgc
    35401 tgcaaacgcg cgttgtcctg acgaccactt aacaccagca gcagattatg caggcaaagc
    35461 acttcctgat tgattgggca ggcgcgaaga ctttgttggc aaaaatccag acccgcgtcg
    35521 aagttaccat tacgcgccgc cagtcgtgcc agaccataat agcctccggc tttactgttg
    35581 ccgctccaga ccgcacgcca gaaatcctct tcggcttgtt gatattgtcc ctgacgttcg
    35641 taagcactgg cgcgaatcaa actcgcctgt ccgcactgcg gatttttgtt cagcgcatgt
    35701 gcgcgtttca gagcctgagt ggcatacgcc accgcttgcg ggaaatctgc gcggttatac
    35761 tccagcatcg ccagcgccag attacagcgg taatccagcg gatccagcgc cacgccgcgc
    35821 aggtagtaat cgaacggtga acggctggcg tgatgatatt gctccagatg ctgaccgata
    35881 aaccaggctt catctgtact ggtaatgtct tgtgctgaca gtggcgcttt ggcgacgtcc
    35941 ggcaacggca actcttgcgg ctgatgttcc tgataactca gtacaatatt gccgtcggca
    36001 tcagagagct caatggtcag tctttcagga ttgataccgt gtaacacgcc ctggatggcg
    36061 gtcgctggtg tcagggccac ggcatcatcg agcaacgcgt cgcatttgcc gatttcgcgg
    36121 atcgccaggc ggtatccgtt caacggagag atggcataca gcccccactc aatcccccgc
    36181 tcactacgct ggagtttgat taccgcatcg cgggaggcgt tttgcaccat gcccagagaa
    36241 tggtaaggca ggaaaaactg ctcaaaacgc ttctcttcgt aagcatcaag ccgggtaaaa
    36301 tcaggctggt tatcggcaaa aataccggtc atcagttcga tatacgggcc gttattatca
    36361 gttaggctct tatcccacgc ctggccaaat tcactgtgtc cccagctcca ctgcttttta
    36421 cctggcgcaa tatggtggtt ggcaacgtgt agcaaaccgc catcttcatc gtgacaccac
    36481 gcgccaacaa aatcgtactg tgatttttca gccatatatg aggttggaac gggcacattt
    36541 ttatagcgag aaatgtccac tccagcggag tagtccactt tgtagtaagt gccggtggcg
    36601 atagggaaag cggagacggc ccgtttgccg tgatcaaaca ccgccgttac atccggcgga
    36661 aagacgctct gatgcccttc accccctttc actgccgggt tggcccacca caagaaatga
    36721 cgcggcgtgg cgttgccgtt atagacgcgg ctggcgattt ccagcgccgc ccggtcaggg
    36781 cgcagggtga aacctgtcat cacctgtaaa ccatgcatcg gctccgtttc gcctacccac
    36841 accgtctgtg cgccgtcttc atgggcttcg agggtgaaat caacgggcat aaaggtggtc
    36901 gggcgatggt gttgcggcca gttaaactca atcccgccgg agatccacgg tcccagcagc
    36961 cccaccagcg caggtttaat gacttcattg tgatagacaa aatcgcgctg tttcacttta
    37021 tcccatgcgc gatgcacccg accgcccaat tccggcagga tcatcacttt gatgtagtcg
    37081 ttttccagcc acaccgcctg ccaggatttc agggttttct gctcgctcag cgtatcggtc
    37141 acgccgtagg gataaaccgc gccggacgat ccctgataaa cgcgattttc caggaacatg
    37201 ggatgtatat cctgcggccc ggtttcatag gtcgggatct caacgcgctc ttgccacact
    37261 tttactggag tcatggtgcc ctcaatatta acaagacata ctgaattaaa agatttgtgg
    37321 cagtgtattg aacaatctgg caatgttttc gcggaataat cacgcaatta actaaacaag
    37381 gtttagtgaa gatgagagcc tgcattaaca atcaacagat tcgccaccat aacaaatgcg
    37441 tgattctgga actgctgtac cggcaaaagc gcgccaataa atcaacgctg gcccggctgg
    37501 cgcaaatttc gattccggca gtcagtaata ttttgcagga actggaaagc gaaaaacggg
    37561 tggtgaatat cgacgatgaa agccagacgc gcgggcatag tagcggtaca tggctgattg
    37621 cgccggaagg tgactggacg ctgtgtctga acgtgacgcc caccagtatt gagtgtcagg
    37681 ttgctaatgc ttgtttaagt ccgaaaggtg aatttgagta tttacagatt gatgcaccga
    37741 cgccgcaggc gctgctgtcc gaaatcgaaa aatgctggca tcgccaccgt aaattgtggc
    37801 cggaccgcac catcaatctg gcgctggcaa tccacggtca ggttgatcca gtgactggag
    37861 tgtcgcaaac catgccgcaa gcgccgtgga caacgccggt tgaggtgaag tatctgctgg
    37921 aagagaagct gggcattcgg gtgatggtcg ataatgactg cgtgatgctg gcgctggcgg
    37981 aaaaatggca aaataattcg caggaacggg atttctgcgt gatcaacgtt gattacggca
    38041 ttggctcgtc gttcgtgatt aacgagcaaa tttatcgcgg cagtttgtat ggtagcggac
    38101 agattggtca caccatcgtt aatccggatg gcgtcgtctg cgactgtgga cgttacggct
    38161 gcctggaaac cgtcgcctcg ttaagtgcat taaaaaaaca ggcgcgggta tggctaaaat
    38221 cacaaccggt taatactcag cttgatcctg aaaaactgac tacagcgcag ttaatcgctg
    38281 cctggcaaag tggagaaccg tggatcacca gctgggttga tcgctctgcc aatgccattg
    38341 gtttgagtct gtataacttc ctcaacatcc tcaatattaa tcagatttgg ttgtacggtc
    38401 gcagttgtgc ctttggtgag cactggctta atactattat tcgccagaca ggatttaacc
    38461 cgttcgaccg cgacgaagga ccgagcgtga aagcgacgca aattggcttc gggcaattaa
    38521 gccgcgcaca acaggtgctg ggaattggct atttgtatgt tgagacgcag ttacgacaga
    38581 tttgatggcg cgataacgta gaaaggcttc ccgaaggaag cctttttcaa cagattaacc
    38641 gtttcgctta tgcgtaaacc gggtaacgtg cgcagatgtc gagaacttta cctttgatgc
    38701 gctcgataac ggcttcatca ttgatgctgt ccagcacgtc acacatccag ccagccagtt
    38761 ctttcgcttc tacttctttg aagccgcgac gcgtaattgc cggagtaccc acgcgaatac
    38821 cggaggtcac aaacgggctc ttcggatcgt tcggtacgct gtttttgttg acggtgatgt
    38881 tagcacggcc cagagcggcg tctgcttctt taccggtcag gtttttatcg accagatcaa
    38941 ccaggaacag atggttatca gtaccgccgg aaaccacttt gtagccgcgt tccaggaaca
    39001 cttctaccat cgctttagcg tttttagcga cctgctgctg gtaagttttg aactcaggct
    39061 ccatcgcttc tttcagagca accgctttac cggcgattac gtgcatcaac ggaccgccct
    39121 gaccaccagg gaaaacggca gagttcagtt ttttgtacag ctcttcgcta ccacctttcg
    39181 ccaggatcag accgccgcgc ggacccgcca gggttttgtg agtggtggta gtaacaacgt
    39241 gagcatgagg aaccgggttc gggtagacgc cagcagcaac caggcccgca acgtgcgcca
    39301 tatcaacgaa caggtaagcg ccgatgctgt cagcgatttc acgcattttc gcccagtcca
    39361 ccacgccgga atatgcagag aagccaccga taatcatttt cggtttgtgt tctttggctt
    39421 gtttttccag atcggcgtag tcgatatgac cggtagcatc aataccgtaa ggaacaatgt
    39481 tgtacagttt accggagaag ttaaccggag aaccgtgagt caggtgaccg ccatgcgcca
    39541 ggttcatacc cagaacggta tcacctggtt ccagcagcgc ggtgtagacc gcaaagttag
    39601 cctgggagcc ggagtgcggc tggacgttag cgtagtcagc gccgaacagt tctttcgcac
    39661 gatcgatcgc cagttgttca acgatatcaa catactcgca accaccgtag tagcgtttgc
    39721 ccggataacc ttcagcatat ttgttggtca gctgagaacc ctgcgcctgc attacgcgcg
    39781 ggctggtgta gttttcggag gcgatcagtt cgatgtgctc ttcctgacgt actttttcct
    39841 gctccatagc ctgccacagt tcggcatcat aatcggcaat gttcatttca cgctttaaca
    39901 tccgcatctc ctgactcagc taacaataaa atttttggcc tttataggcg gtcctgttgg
    39961 acaacggcga acagtataac cgaatcattg tgcgataaca ggtcttgaca aaggaattta
    40021 cgcaaacgat taccttcagg ctacgcaagg ctttggagaa taaagagctt gcaaccggaa
    40081 acggatttct tttcaggttt gtgatgcaaa tttttcactt catcacattc tttctgaaaa
    40141 acaccaaaga accatttaca ttgcagggct attttttata agatgcattt gagatacatc
    40201 aattaagatg caaaaaaagg aagaccatat gcttgacgct caaaccatcg ctacagtaaa
    40261 agccaccatc cctttactgg tggaaacggg gccaaagtta accgcccatt tctacgaccg
    40321 tatgtttact cataacccag aactcaaaga aatttttaac atgagtaacc agcgtaatgg
    40381 cgatcaacgt gaagccctgt ttaacgctat tgccgcctac gccagtaata ttgaaaacct
    40441 gcctgcgctg ctgccagcgg tagaaaaaat cgcgcagaag cacaccagtt tccagatcaa
    40501 accggaacag tacaacatcg tcggtgaaca cctgttggca acgctggacg aaatgttcag
    40561 cccggggcag gaagtgctgg acgcgtgggg taaagcctat ggtgtactgg ctaatgtatt
    40621 tatcaatcgc gaggcggaaa tctataacga aaacgccagc aaagccggtg gctgggaagg
    40681 tactcgcgat ttccgcattg tggctaaaac acctcgcagc gcgcttatca ccagcttcga
    40741 actggagccg gtcgacggtg gcgcagtggc agaataccgt ccggggcaat atctcggcgt
    40801 ctggctgaag ccggaaggtt tcccgcatca ggaaattcgt cagtactctt tgactcgcaa
    40861 accggatggc aaaggctatc gtattgcggt gaaacgcgaa gagggtgggc aggtatccaa
    40921 ctggttgcac aatcacgcca atgttggcga tgtcgtgaaa ctggtcgctc cggcaggtga
    40981 tttctttatg gctgtcgcag atgacacacc agtgacgtta atctctgccg gtgttggtca
    41041 aacgccaatg ctggcaatgc tcgacacgct ggcaaaagca ggccacacag cacaagtgaa
    41101 ctggttccat gcggcagaaa atggcgatgt tcacgccttt gccgatgaag ttaaggaact
    41161 ggggcagtca ctgccgcgct ttaccgcgca cacctggtat cgtcagccga gcgaagccga
    41221 tcgcgctaaa ggtcagtttg atagcgaagg tctgatggat ttgagcaaac tggaaggtgc
    41281 gttcagcgat ccgacaatgc agttctatct ctgcggcccg gttggcttca tgcagtttgc
    41341 cgcgaaacag ttagtggatc tgggcgtgaa gcaggaaaac attcattacg aatgctttgg
    41401 cccgcataag gtgctgtgat ttgatgttgc cggatggaaa catccggcaa cccttgacgc
    41461 ggtttaaatt gccgcgtcgt cctcttcacc ggtacggatg cgaatgaccc gtgccacgtc
    41521 aaagacgaag attttaccgt caccgatttt gccggtttgc gccgtgcgaa taatggtatc
    41581 gacacaggta tcgacaatgt cgtccggtac gacaatctca attttcactt tcggcagaaa
    41641 atccaccata tactccgcgc cgcggtacag ctcggtatgg cctttctggc ggccaaaacc
    41701 tttcacctca gtgaccgtca tgccggtaat gccgacttca gccagtgctt cgcggacatc
    41761 gtccagcttg aagggtttaa taatcgcatc aatctttttc atgctattcc ttgaaaaggt
    41821 cgcctgtctt ttgatctgct aaacgtaaca cataacgcca attcattcct tgaaatcgtt
    41881 tgcatccagc tcgtgtcggg aaagcagttt ataaaattct gtccggttgc gccccgccat
    41941 tctcgccgcg tgggtgacgt tgcctttggt aatttgcagc agcttacgca aatagttgag
    42001 ttcaaactga ttacgtgcct caacaaaggt tggcagcgcc gtattttcac cctccagcgc
    42061 ctgctctact aatgcatcgc taatcaccgg agatgaggtc agcgccacgc actgttcaat
    42121 cacgttgacc aactggcgca cattacccgg ccagctcgcg gtcatcaggc gtttcatcgc
    42181 atcggtggaa aacgcgcgga caaacggttt atgtcgctct gccgcctggc gcaacaggtg
    42241 atttgccagt agcggaatgt cttctgtgcg ctccgccagt gccggaattt tcaggctgac
    42301 aacgttgagg cggtaataga gatcttcgcg gaattccccg cgcgccatcg cttttggcag
    42361 atcacggtga gtggcagaaa taatccgcac attgatatca atatcgcggt tactgcccag
    42421 cgggcgcact ttacgctcct gcaacacgcg caacagtttg acctgcaacg gcgcaggcat
    42481 atcgccaatc tcatcgagaa atagcgttcc gccttccgcc gcctggaata aaccttcgcg
    42541 attgctgaca gcgccagtaa acgcgccacg cgcatgacca aacagctccg actccagcaa
    42601 ttgctcaggc aatgcaccac agttaatagc aataaatggt ttgctattgc gcgggctggc
    42661 gttgtggata gcctgggcga aaatctcttt cccggtgccg ctctggccat taatcaaaac
    42721 gctgacgtct gattgcgcca ccagccgcgc ctgttccagc aaacgcagca tcagcgggct
    42781 gcgggtgaca atcgcctcgc gccagcgttc gtcggtggct ggcgcggatt gttccagcgc
    42841 atcatcaatt gcctgatata gcgcgtcttt atcaacaggc ttggtgagga aactaaaaac
    42901 gccctgctgt gttgcagcaa cggcatcggg aatagaacca tgcgcggtaa gaataattac
    42961 cggcattccc ggctgcactt tctggatttc agcaaacagc tgcataccgt ccatttcatc
    43021 catccgcagg tcgctgatga ctaaatctac tttttcgcga ttcagtaccc gtaatccttc
    43081 agcgccactt tccgccgtga ccacgctgta gccttcgctg gtcaggcgca ggccaagcag
    43141 tttcagcaat cccggatcgt catcgaccaa taataaatgc gcaggtttat ggctcatcag
    43201 gagtgacctc atgggtggat ggcgcgggct tttcactctc gtgtggcgta tccggcgaga
    43261 aatttccagc cggtttgcgg gtcgagagct ggcgttcaat atcagtcagg ttttccagct
    43321 tgcgggtggt gagttccagt tgctgctgta gaacgtgatg ttgctggcgc aatgtatcca
    43381 gctcgctgtc gctggactgc tggagtttac tgtagcgttg gcgctcttcc gccagttgca
    43441 gttgcagcgc ctgaccgtcg cgccagagtt gatacagtgg gcgaacctgt gccgggatct
    43501 cggtacttaa cgcttcaata cgcgcgacca gctggcggcg ctcatatggc gtaattttgg
    43561 cgtcggcgag caaaatccct tgtttaaagg tattttgcca gctgccgtcg tcatattggc
    43621 gagcttgctg acgcgactgc gcaggcatta aacgatcagc acaatccatc gcccgcagcc
    43681 agtaaagcgg attggtttcg gttgatttac cttgcagcgc ccagatgtcg ctacattcag
    43741 tagaaagata gtcagccagt tgataaaccg gaattttttc ttctgctggc gtatcaatgg
    43801 ctggcttatt gtgattctgc acgcaaccta gcaatgccag acatggcagc cctgccagcc
    43861 acagccgtcg gggcaataat cgttgaaaaa tgtgtcgcat attcaccaga cttaaagcct
    43921 atcccagtgg gcgtaattgt tgcagacagt ttggacatgg acagcgcgga gaaaccggag
    43981 cgtacatgtc gtacgtgagg atttcgagca ctgcccgggg ccgaactgac aaataaaata
    44041 gcctgatggg ataggctgtt agcattattt cgtgtttttc gacgacggta attcaatgcg
    44101 gaaacaaacg tcttgcccgc tctcgtcgac cagatacagt tccccttgca tacggcgaat
    44161 acaatccctg gcaatgctta atcccagacc gctgcccttc accgcccctt ttcgctggtg
    44221 gcttccctga aaaaagggtt cgaagatcat ggcgcgttcc tcttgcggaa tgggcgtgcc
    44281 tgtattgatg acatcaatat aaacccgcgc accatgtaaa ctgctgcgaa ggcaaatgtt
    44341 accggattca gccccgtagt gcaccgcatt ggagtaaaga ttatccagta cgctcatcag
    44401 cagcattggc tccgccaggc aagctgttgc tttgagatcg acgtcggtat gcatcatttt
    44461 agcccgtgcg ggcaggctat gagcagaaac tactgtctcc accagcggtg ctaactcaac
    44521 attatctagt tccaccgcac tgtccgcctg tttacggttg taatcaagca gttgttcgat
    44581 cagtttttgc aaattgcggc tgctgctatc aagaatgctc accacctctt tttgctctgg
    44641 cgtaagcggc ccgacaacct gatcagccag taattcagtg ccctcgcgca tactcgccag
    44701 tggcgttttt aattcatgag ataaatgtct taaaaattga tggcgttggg attccagcca
    44761 tgacaggcgc tcacttaacc aaagaatacg ttgcccaacc gagcgtaact cgctcggtcc
    44821 actgaacgaa acgctattgc ccagagaacg cccttccccc aaccgattga tcatccgttc
    44881 aatatttttc accggcccga taatcatccg cgtgaaaagc agtaccatta ccagactcac
    44941 cagaaatagc accagcgatt gccaaccaaa atattgccca cgttcggcga tttcacgctg
    45001 aagttgctgc ccacgagaga acactactgt gcgcgtggcc tgtaccattt cggtattggc
    45061 actggcaaag gcttccagac gcgcggcagc agcagcatcg ggaccactgt tgttgcactg
    45121 aagttgggcc agattgttca agtcctgacg caatgcctgg taaagtttat cgtctggcag
    45181 tacgcctgcg tgggcatcga gcatttcgct gtaacgcttg cgctggcttt gataaacctt
    45241 cgcaagcgta gggtcatcca gcacgcaata ctgacggtag ctacgctcca tctccagcgc
    45301 cgcgttggtc attgcttcgc tgcgccgggc atcgataagc gtggtgcggt tgaccagcgc
    45361 cgcctggtcg ctaagcgcat tcaggctttg ccaggcttgc catgccagca ccaacagggg
    45421 cagcagaatc agcaaaaatg ccagcattac cagttgtcgt aatgagcggg gaaaaacggg
    45481 ccagcgtttc aaggtgttac tctcgtcaga cgcgaatagc ctgatgctaa ccgaggggaa
    45541 gttcagatac aacaaagccg ggaattaccc ggctttgtta tggaataagg cggtgcctaa
    45601 ctcgacgttt cgcccgatgg ttgatatagc tacgctgata tcagaagttg gacggcaggc
    45661 accttgttgt gcgtcattcg aattttatgt agcacgtccc gaaggggctg acataagtcg
    45721 gtgaatgagc cactgattac tattatgcag taactgtgcc aataaagaaa atagtttggt
    45781 aacgtactga ttatacgttg ctttgagggg tttatggctt ccccgctatt tgaatgtaaa
    45841 tcagccactg tgtcgctaaa aagagacaac ttaagaaaaa cttattagat tacaataaaa
    45901 tcaatcattt aagtgtcgcc agaaagcgac acggtaaacc acccattgtc gtgatttaca
    45961 gacacaaaaa agctcccgaa gttgggagct tataatagcg gttgtattta atgccggatg
    46021 cggcgtgaat gcctcatccg ggctgcaaaa ccaatgagtt tgacggctta ccccaactgc
    46081 ttacgtgcat tgcggaaaat gcgcatccat gggccatcct cgccccagtt ttcaggatgc
    46141 caggagttgc tgacagtacg gaaaacacgt tccgggtgcg gcatcataat ggtgactcga
    46201 ccgctttcag tcgtgaccgc cgtaataccg ttcggagaac cgttcgggtt agccgggtag
    46261 gtttcagtga ctttgccgaa gttatcgaca tagcgcagtg ccaccagccc tttgctttcc
    46321 agtgccgcca gatgcgccgc atcacgcact tccacgcgcc cttcaccatg agagacagca
    46381 atcggcattt gcgagcccac catcccctgc aacagcagcg acgggctttg ggtcacttca
    46441 accaagctga aacgcgcttc aaagcgatcg gaggtattgc gcacaaaacg tggccacaac
    46501 tcactacctg ggatcagttc acgcagatta gacatcatct ggcaaccgtt acataccccc
    46561 agcgccagcg tttgcggacg gtggaagaag gttgcaaact catcgcgtac acggtcattg
    46621 aacaggatag acttcgccca accttcaccg gcacccagca catcaccgta ggagaaacca
    46681 ccgcacgcga ccagggcgtg gaaatcctcc aggcccgtgc gtccagccag caggtcgctc
    46741 atatgcacgt cgatagcatc gaagcctgca cggtggaaag ctgccgccat ttcaacatgc
    46801 gagttcacgc cctgctcacg cagcacagca actttcggac gtgcgccagt ggcaatatac
    46861 ggtgctgcca catcttcgtt gatatcaaac gacagtttca cattcaggcc cggatcggcg
    46921 tcgtttgatt tcgcctgatg ctcctgatcg gcacactccg ggttgtcacg caggcgctgc
    46981 atctgccagg tagtttctgc ccaccagaca cgcaacgtag tgcggctttc gctgaataca
    47041 gtctgcccgt tggcggtaat cacaaaacgg tcaccggaaa ccgcctgccc aacataatgg
    47101 acgcaatcag caagcccatg ctgtgccagt acggactcga ccgcttcacg gtcagcggca
    47161 cgaacctgaa tgaccgcacc cagttcttcg ttaaacaacg ccgccaggcg atcgtcaccc
    47221 agagtggcga tatccgcgtc aatgccgcaa tgaccagcaa aggccatttc cgccagcgtt
    47281 accagcagac cgccatcaga gcggtcgtga tacgccagca acttacgctg tgcaaccagc
    47341 gcctgaatcg cgtcatagaa gcctttcagt tgcgcgacat cgcgtacatc tgccggtttg
    47401 tcgccaagct gacgataaac ctgcgccagc gccgttgcgc ccagcgcgtt attgcctttg
    47461 cccaaatcaa tcagcagcag tgcgttatct tcggtagaaa gctgcggcgt gatggtgtga
    47521 cgtacatctt ccacgcgggc aaatgcagaa atcaccagcg acagcggcga cgtcatttcg
    47581 cgctcttcgt taccttcctg ccagcgggtt ttcatcgaca tagagtcttt acccaccggg
    47641 atcgtcaggc ccagcgccgg acaaagctct tcgcccacgg ctttaacggc ttcatacagg
    47701 cccgcatctt caccagggtg gcctgccgcc gccatccagt tggcggaaag tttaatgcgt
    47761 ttgatatcgc caatctgtgt tgcggcaata ttggttaacg cttcaccgac cgccagacgg
    47821 gcagaggcgg cgaaatccag cagcgcaacc ggcgcacgct cgccaatcgc catcgcttca
    47881 ccgtagtagc tgtcgaggct ggcggtagtg accgcgcagt tagcgaccgg cacctgccac
    47941 ggccccacca tctgatcgcg cgccaccatg ccggttacgc tgcggtcacc gatggtcacc
    48001 aggaaagttt tctccgccac ggtcggcaga tgcagtacgc gtttcaccgc gtctgcaatg
    48061 gtgatccctt cacggaccag cgcatcgccc ttcgctttca gcgtttgtac atcgcgggtc
    48121 atcttcggcg ttttaccaag caggacgtcc agcggcagat cgatcggctg attatcaaaa
    48181 tgacgatcgt gcagagaaag atgcagttct tcggtcgctt caccaatcac cgcgtagggt
    48241 gcgcgctcac gcttacacag ttcgtcaaac agcggtaatt gatcggcagc aaccgccagc
    48301 acgtagcgtt cctgggattc gttacaccag atttccagcg ggctcatgcc cggttcgtcg
    48361 cttagaatat cgcgcagttc aaatttaccg ccgcgcccgc cgtcgctcac cagttccggc
    48421 atcgcgttag aaagaccgcc agcgccgacg tcgtggataa acaggattgg gttggcatca
    48481 ccaagctgcc agcaacggtc gatcacttcc tggcagcgac gctccatctc cgggttgtcg
    48541 cgctgtacgg aagcaaagtc gaggtcggca tcagactgac ctgacgccat ggaagacgcc
    48601 gctccgccgc caagaccgat gttcattgcc gggccgccga gaacgaccag tttcgcaccg
    48661 acgttgatct cgcctttttg tacgtgatcg gcgcgaatgt tgccgatccc gcccgccagc
    48721 atgatcggtt tgtgataacc gcgcagctct tcgccgttgt ggctgttcac tttttcttca
    48781 taagtacgga agtagccatt cagtgccgga cgaccaaatt cgttgttaaa cgccgcgccg
    48841 cccagcgggc cttcggtcat gatgtccagc gcggtgacaa tgcgctcagg cttaccgaaa
    48901 tcttcttccc acggctgttc gaagccagga attcgcaggt tggaaacgga gaaaccaacc
    48961 aaacccgctt tcggcttcgc gccgcgcccg gtggcacctt catcgcggat ttcaccgccg
    49021 gaaccggtcg ccgcgcccgg ccacggagaa atcgccgtcg ggtggttgtg agtttcgact
    49081 ttcatcagaa tatgcgccgg ttcctgatgg aaatcgtagc ggcccgtttc gtggtcagca
    49141 aagtagcggc ccacttcaga accttccatt acggcggcgt tatctttata agcagagaga
    49201 acgtgatctg gcgtggtttc gaaagtattt ttgatcatct tgaacagcga tttcggctgc
    49261 tgttcaccgt cgataatcca gtcggcatta aaaattttgt ggcggcagtg ctcagagttc
    49321 gcctgagcga acatatacag ctcgatgtcg ttcgggttac gaccaagctt tgtgaaagca
    49381 tcctgcagat agtcaatttc atcttccgcc agagccaggc caagacgcag gttagcgtcg
    49441 atcagcgcct gacggccctg ccccagcaaa tcaacgctgg taaccggagt cggttgatgg
    49501 tgggcaaaca actgctctgc atcatctaaa gcaaaaaaga cagtttccat catgcggtcg
    49561 tgcagttcag cggtaacctg ctgccattgt tcattggtca gcgtaccggc ttctatatag
    49621 taagcaacgc cgcgctcaag gcggtttacc tgttgtagcc cgcagttatg agcaatatcg
    49681 gtcgctttcg aagaccaggg agagatggtg ccaggacgcg gggtcaccag caggagtttg
    49741 ccttgcgggg cgtggctggc gagtgccggg ccatatttca gcaggcgttc aagttgtgcg
    49801 tgctcatcat cgtttaacgg cgcattgagg tcagcaaaat ggacatactc ggcgtaaata
    49861 ttgtgaaccg ggagcctggc agcctgaaaa cgtgccagca gtttgttgat tcggaatgcc
    49921 gacagtgcag gcgaaccacg cagaatttcc atcataagtc tctcgtcttc taagctttcg
    49981 gtgtacccaa ggggggaaac gggcgtcatt ataaagaatc tgatgcgctg acgaaaccgt
    50041 ttgcgtggaa ataaaatcac catcgtgaat tagcaacgcg tgccgccaat ggctgtaata
    50101 agttgccatc tggcgcaggt ttacgcaaaa tgccgctcat ttatgagtaa acctttcact
    50161 attattacat tttttcaagc tgggacgcgc acgacacaga gaattaacta attgaaaaaa
    50221 ttaaagatta attatctgtt catcggcatt ctggcactgc tgctcgcggt cgctctctgg
    50281 ccatccattc cctggtttgg taaagccgac aaccgtatcg ccgccattca agcgcgggga
    50341 gagttgcgtg tgagcaccat tcatactccc ctgacgtata acgaaatcaa cgggaaacct
    50401 tttggcctgg attacgaact ggcgaaacag tttgccgatt acctcggcgt aaaactgaaa
    50461 gtgaccgtgc ggcagaatat cagccagctg tttgacgacc tcgataatgg taacgccgac
    50521 ctgctggcgg caggacttgt ctataacagt gagcgggtaa aaaattatca gcctggccct
    50581 acctattatt ccgtgtcaca acaactggtt tataaagtgg gtcagtatcg cccacgtacg
    50641 ctgggcaacc tgacggcgga gcaactcacc gttgcaccgg gtcatgtggt ggttaacgat
    50701 ctccagaccc tgaaagaaac aaaattcccg gaattaagct ggaaggtaga cgacaaaaaa
    50761 ggctctgcgg aattaatgga agatgtcatc gaaggaaaac tcgattacac cattgctgat
    50821 tctgtcgcca tcagcctgtt tcagcgcgtt cacccggagc tcgccgtagc gctcgatatc
    50881 accgatgaac aaccggtgac ctggtttagc ccgttagatg gcgataatac cctttccgcc
    50941 gccctgctcg acttcttcaa cgaaatgaat gaagacggta cgctggcacg cattgaagag
    51001 aaatacctgg ggcatggcga tgattttgat tacgtcgata cgcgcacatt tttacgcgcc
    51061 gtcgatgcgg tactgccgca gttaaagccc ctgtttgaga aatacgccga agaaattgac
    51121 tggcgtttgc tggccgctat tgcttatcag gaatcgcact gggatgcaca ggccacttca
    51181 ccgacgggtg tgcgcggcat gatgatgtta accaaaaata ccgcgcaaag cctcggcatt
    51241 acggatcgta ccgatgccga acagagcatc agcggtggcg tgcgttattt gcaggatatg
    51301 atgagtaaag tgccggaaag tgtgccggag aacgagcgga tctggtttgc cctcgctgcg
    51361 tacaatatgg gctatgcgca tatgcaggat gcccgcgccc tgacggcaaa aaccaaaggg
    51421 aatcctgaca gttgggctga cgtaaaacag cgtctgcctt tacttagcca gaaaccctat
    51481 tacagcaagc tgacttacgg ctacgctcgt gggcatgaag cctacgctta tgtcgaaaat
    51541 attcgtaagt atcagattag cctggtgggt tatctgcaag agaaagagaa gcaggctaca
    51601 gaagcggcga tgcaactggc gcaggattat ccggcggtat cgcctacgga gttgggcaaa
    51661 gagaaatttc cttttctctc gtttctttcc cagtcgtcat caaactattt gacccattct
    51721 ccctctctgc tgttttccag gaaagggagt gaagagaaac aaaattaatc cgtcgaggat
    51781 tgcgcttttt tctgcgcttt aatttcctgg cggcgcatgc gaaagaagtc actgagcaac
    51841 gccgcgcact catccgccag tattccttcc gtaatttcca ctcggtgatt catacccgga
    51901 tgatgcagca catccattaa agatcccgca gcgccagttt tcgcgtcacg cgcaccaaag
    51961 accacgcgac caatgcgact gtggatcatc gctccggcac acattacaca tggttcaagc
    52021 gtgacataca acgtggcgtc gatcagacga taattttgca tcaccagacc gccctgcctc
    52081 agggccatga tttctgcatg tgcggtggga tcatgtcgac caatcggacg gttccagcct
    52141 tcgccaataa cacgattgtt atgcactaat accgcgccga ccggtacttc ccgctcatcc
    52201 caggcacgct tcgccagcgt cattgcatga cgcatccagt attcgtggct aaattcgact
    52261 tcagacaaaa agaaaactcc ggttataaaa gcgcggcgca ttatacacgg acgctatgct
    52321 ttactctatt ccagttgctg gagttcaccg cgcggggtta ctcgccagcg atgctggcaa
    52381 aagtaaagca gcgggttgtc ctgattactg tcgctatagc cactgtacag ccgcagcggc
    52441 gtgccgattt tgcgctccag ttgcgcgacc ttttcatgcc cgaggcaacg catcgtcaat
    52501 acccaaccac catagccacg ctgaatttgg ctggcgataa gattcacccg cggcagccag
    52561 ggtgtatcga aatagaccgc ttcaaccagc ggctgtggag aaccggtaat caaccagata
    52621 tcagcatcgg aacttaacag gtaggtggtt aatcgctcct gaaccagcgg aaaggcggta
    52681 acattgtcac gaaaccagcg tacgaaatcg gcctgcaacg cctgtaaacg tgcttcgctg
    52741 tgaccaaagg tgcatcccca cagaagcaga ctcatcggcc agcgtgccgc acgacctttt
    52801 atcaataacg caatggctat aatcggcaac aacggcagga caagtaacgc attcagcggt
    52861 tggcgacgca gtaaatagcg cagaaaactg ccgaacatat cctgctgatg caatgttcca
    52921 tctaagtcaa aaaacaccac acgacgctcg tgagttgcca aaccatactc ctctggactg
    52981 aaacatcctg attaattttc gctactacat agcctaacag atagatcatc acttttccgg
    53041 caacaatcga ccgcatcatg gctaactgga atttttaatt catggcaatt agcggcaatg
    53101 gaatataaaa ttcactcgcg tgtgtctcat attgcctgtt gtcgccacta tttcatcgcg
    53161 aggaaagata tgaacggctt acttcgtatc cgtcagcgtt accaggggct tgcccagagt
    53221 gataaaaaac tggcggatta tctgctgcta caacctgata cggcgcgcca tttaagctct
    53281 cagcaactgg ccaacgaagc cggagtcagt cagtccagcg tcgtgaagtt cgcgcaaaaa
    53341 ctcggctata aaggttttcc ggcgcttaag ttggcgttga gtgaagcgct ggcaagccag
    53401 ccggaatcgc cctcagtgcc cattcataac caaatccgcg gtgatgatcc gttacggctg
    53461 gtcggcgaaa aactgattaa agaaaatact gccgctatgt acgcaacgct aaacgttaat
    53521 agtgaagaga aactgcatga atgcgtaaca atgttgcgct ctgcgcggcg gataattctg
    53581 accggtattg gcgcttcggg tctggtggcg caaaactttg cctggaagct gatgaagatt
    53641 ggcttcaatg ctgccgcagt gcgcgatatg catgcgctac tcgctaccgt acaggcgtcg
    53701 tcccctgacg atctgttatt agccatttct tacaccggtg tacgacgcga gttaaacctg
    53761 gcggcagatg agatgctgcg agtgggcgga aaagtgctgg cgattaccgg ctttactccc
    53821 aatgccctgc aacagcgtgc ttctcattgc ctgtatacca ttgccgaaga acaagcgaca
    53881 aacagtgctt caatctctgc ttgtcacgcg cagggaatgt taacggattt gctgttcatt
    53941 gcgctgattc agcaggatct ggaactggca ccagaacgta ttcgtcatag tgaagcgctg
    54001 gtgaaaaaac tggtctgagt aaagaatgcg cgtataatgc ccgcccggtt tgtgttgttt
    54061 tgagagtttc cttatggcgc tgttaatcac taaaaaatgc atcaattgtg atatgtgtga
    54121 acccgaatgc ccgaatgagg cgatttcaat gggagatcat atctatgaga ttaacagcga
    54181 taagtgtacc gaatgcgtag ggcactacga gacaccaacc tgccagaagg tgtgcccgat
    54241 ccccaatact attgtgaaag atccgcaaca tgtcgagaca gaagaacagt tgtgggataa
    54301 atttgtgctg atgcaccacg cggataaaat ttaatattct actctggaag tagagtatta
    54361 attattttac tgggaagcct taacgccatt atatttattt aattgatgac attagcataa
    54421 taattcacta agttaattta tatagtatct gcccagacac ttatttatag ttattaaagg
    54481 cgcgtccgat tggttcaccg gacgcacctt aagtacgttt ccttgtgtta taagaacaga
    54541 aggatcagct gtaaaacagc aatgatgatt ttgatgaccc gtttaatcag gtatcggcaa
    54601 tcagtcattc gtttttcctt aaacaaggaa tgcagccgtt cagattagcc cttacatctc
    54661 cccaaaactg aacgtgcgag ttattggaat cgagtgctgc actccacacc agagctttga
    54721 cgacaccact cgtttcaatg ggggaattca gtggcatggt gtaaagcaca gcaaaattgc
    54781 caataacgaa accgatttta atgtacttat caaaaagcgg tcacgttaat cttttcatca
    54841 atgtcgattt cttcgtaaag gctcgcgttt tttatgtcag agattgcaag ttgcttgtac
    54901 aggtcaacga aattgatcca taatcatttt cgttggaatc gtgtgctgca caaaattaaa
    54961 gttaaaaagt aaaacccccg ttccttacca gttcgggggt tttactttat aa
//
LOCUS       DBFIFM010000008        52965 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.008, whole genome shotgun sequence.
ACCESSION   DBFIFM010000008 DBFIFM010000000
VERSION     DBFIFM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.008"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            20..2263
                     /gene="pdeF"
                     /locus_tag="N7C74_000443"
     CDS             20..2263
                     /gene="pdeF"
                     /locus_tag="N7C74_000443"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HGU2430383.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDVKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRTVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2302..3843)
                     /gene="ppx"
                     /locus_tag="N7C74_000444"
     CDS             complement(2302..3843)
                     /gene="ppx"
                     /locus_tag="N7C74_000444"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HGU2430384.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3848..5914)
                     /gene="ppk1"
                     /locus_tag="N7C74_000445"
     CDS             complement(3848..5914)
                     /gene="ppk1"
                     /locus_tag="N7C74_000445"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HGU2430385.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6085..6723)
                     /gene="purN"
                     /locus_tag="N7C74_000446"
     CDS             complement(6085..6723)
                     /gene="purN"
                     /locus_tag="N7C74_000446"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HGU2430386.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHPHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6723..7760)
                     /gene="purM"
                     /locus_tag="N7C74_000447"
     CDS             complement(6723..7760)
                     /gene="purM"
                     /locus_tag="N7C74_000447"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HGU2430387.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVNAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8085..8711
                     /gene="upp"
                     /locus_tag="N7C74_000448"
     CDS             8085..8711
                     /gene="upp"
                     /locus_tag="N7C74_000448"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HGU2430388.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8797..10086
                     /gene="uraA"
                     /locus_tag="N7C74_000449"
     CDS             8797..10086
                     /gene="uraA"
                     /locus_tag="N7C74_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HGU2430389.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10181..10882
                     /gene="hda"
                     /locus_tag="N7C74_000450"
     CDS             10181..10882
                     /gene="hda"
                     /locus_tag="N7C74_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HGU2430390.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11020..11379)
                     /gene="arsC"
                     /locus_tag="N7C74_000451"
     CDS             complement(11020..11379)
                     /gene="arsC"
                     /locus_tag="N7C74_000451"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HGU2430391.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11400..12863)
                     /gene="bepA"
                     /locus_tag="N7C74_000452"
     CDS             complement(11400..12863)
                     /gene="bepA"
                     /locus_tag="N7C74_000452"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HGU2430392.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRNAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13076..14137
                     /gene="yfgO"
                     /locus_tag="N7C74_000453"
     CDS             13076..14137
                     /gene="yfgO"
                     /locus_tag="N7C74_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGU2430393.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14175..15023)
                     /gene="focB"
                     /locus_tag="N7C74_000454"
     CDS             complement(14175..15023)
                     /gene="focB"
                     /locus_tag="N7C74_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HGU2430394.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQES"
     gene            complement(15045..17057)
                     /gene="hyfR"
                     /locus_tag="N7C74_000455"
     CDS             complement(15045..17057)
                     /gene="hyfR"
                     /locus_tag="N7C74_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HGU2430395.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLHLLASNGCESSLLIPLTFGN
                     HTPGALLLAHTSTTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17087..17500)
                     /gene="hyfJ"
                     /locus_tag="N7C74_000456"
     CDS             complement(17087..17500)
                     /gene="hyfJ"
                     /locus_tag="N7C74_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HGU2430396.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVTFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            complement(17493..18251)
                     /gene="hyfI"
                     /locus_tag="N7C74_000457"
     CDS             complement(17493..18251)
                     /gene="hyfI"
                     /locus_tag="N7C74_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HGU2430397.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGQEICDRLLRHLSND
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(18248..18793)
                     /gene="hyfH"
                     /locus_tag="N7C74_000458"
     CDS             complement(18248..18793)
                     /gene="hyfH"
                     /locus_tag="N7C74_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HGU2430398.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPTNALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(18803..20518)
                     /gene="hyfG"
                     /locus_tag="N7C74_000459"
     CDS             complement(18803..20518)
                     /gene="hyfG"
                     /locus_tag="N7C74_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HGU2430399.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVQYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSGARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(20508..22088)
                     /gene="hyfF"
                     /locus_tag="N7C74_000460"
     CDS             complement(20508..22088)
                     /gene="hyfF"
                     /locus_tag="N7C74_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HGU2430400.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGHSATRTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     EHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22093..22743)
                     /gene="hyfE"
                     /locus_tag="N7C74_000461"
     CDS             complement(22093..22743)
                     /gene="hyfE"
                     /locus_tag="N7C74_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HGU2430401.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLSMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22755..24194)
                     /gene="hyfD"
                     /locus_tag="N7C74_000462"
     CDS             complement(22755..24194)
                     /gene="hyfD"
                     /locus_tag="N7C74_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HGU2430402.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVCTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWVLISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(24211..25158)
                     /gene="hyfC"
                     /locus_tag="N7C74_000463"
     CDS             complement(24211..25158)
                     /gene="hyfC"
                     /locus_tag="N7C74_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HGU2430403.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGCAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25169..27187)
                     /gene="hyfB"
                     /locus_tag="N7C74_000464"
     CDS             complement(25169..27187)
                     /gene="hyfB"
                     /locus_tag="N7C74_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HGU2430404.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHEGVTLVARFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISALPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLSGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27187..27804)
                     /gene="hyfA"
                     /locus_tag="N7C74_000465"
     CDS             complement(27187..27804)
                     /gene="hyfA"
                     /locus_tag="N7C74_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HGU2430405.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCVQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGREDPLSLTQEQR"
     gene            complement(28057..28527)
                     /gene="bcp"
                     /locus_tag="N7C74_000466"
     CDS             complement(28057..28527)
                     /gene="bcp"
                     /locus_tag="N7C74_000466"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HGU2430406.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28527..29099)
                     /gene="gcvR"
                     /locus_tag="N7C74_000467"
     CDS             complement(28527..29099)
                     /gene="gcvR"
                     /locus_tag="N7C74_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HGU2430407.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29245..30123
                     /gene="dapA"
                     /locus_tag="N7C74_000468"
     CDS             29245..30123
                     /gene="dapA"
                     /locus_tag="N7C74_000468"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HGU2430408.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30140..31174
                     /gene="bamC"
                     /locus_tag="N7C74_000469"
     CDS             30140..31174
                     /gene="bamC"
                     /locus_tag="N7C74_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HGU2430409.1"
                     /translation="MAYSVHKSRLATVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31387..32100
                     /gene="purC"
                     /locus_tag="N7C74_000470"
     CDS             31387..32100
                     /gene="purC"
                     /locus_tag="N7C74_000470"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HGU2430410.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32268..33131
                     /gene="ypfJ"
                     /locus_tag="N7C74_000471"
     CDS             32268..33131
                     /gene="ypfJ"
                     /locus_tag="N7C74_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HGU2430411.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33146..35161
                     /gene="tmcA"
                     /locus_tag="N7C74_000472"
     CDS             33146..35161
                     /gene="tmcA"
                     /locus_tag="N7C74_000472"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HGU2430412.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTADNDAIFWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQILQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAG
                     SAIITAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            35235..35933
                     /gene="ypfH"
                     /locus_tag="N7C74_000473"
     CDS             35235..35933
                     /gene="ypfH"
                     /locus_tag="N7C74_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU2430413.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36014..36214)
                     /gene="ypfN"
                     /locus_tag="N7C74_000474"
     CDS             complement(36014..36214)
                     /gene="ypfN"
                     /locus_tag="N7C74_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HGU2430414.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36242..37369)
                     /gene="dapE"
                     /locus_tag="N7C74_000475"
     CDS             complement(36242..37369)
                     /gene="dapE"
                     /locus_tag="N7C74_000475"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HGU2430415.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKAVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(37373..37729)
                     /gene="yffB"
                     /locus_tag="N7C74_000476"
     CDS             complement(37373..37729)
                     /gene="yffB"
                     /locus_tag="N7C74_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HGU2430416.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNIITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQKFFHEV"
     gene            38110..38169
                     /gene="ypfM"
                     /locus_tag="N7C74_000477"
     CDS             38110..38169
                     /gene="ypfM"
                     /locus_tag="N7C74_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HGU2430417.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(38268..41381)
                     /gene="acrD"
                     /locus_tag="N7C74_000478"
     CDS             complement(38268..41381)
                     /gene="acrD"
                     /locus_tag="N7C74_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HGU2430418.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATAEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(41545..43245)
                     /gene="narQ"
                     /locus_tag="N7C74_000479"
     CDS             complement(41545..43245)
                     /gene="narQ"
                     /locus_tag="N7C74_000479"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HGU2430419.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGTPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLGRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            43451..45430
                     /gene="aegA"
                     /locus_tag="N7C74_000480"
     CDS             43451..45430
                     /gene="aegA"
                     /locus_tag="N7C74_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HGU2430420.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLIRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            45498..46073
                     /gene="nudK"
                     /locus_tag="N7C74_000481"
     CDS             45498..46073
                     /gene="nudK"
                     /locus_tag="N7C74_000481"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HGU2430421.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLID"
     gene            46199..47242
                     /gene="ypfG"
                     /locus_tag="N7C74_000482"
     CDS             46199..47242
                     /gene="ypfG"
                     /locus_tag="N7C74_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HGU2430422.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSDLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVVVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(47337..49340)
                     /gene="tkt"
                     /locus_tag="N7C74_000483"
     CDS             complement(47337..49340)
                     /gene="tkt"
                     /locus_tag="N7C74_000483"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGU2430423.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALVRQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(49360..50310)
                     /gene="tal"
                     /locus_tag="N7C74_000484"
     CDS             complement(49360..50310)
                     /gene="tal"
                     /locus_tag="N7C74_000484"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HGU2430424.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            50599..52878
                     /gene="maeB"
                     /locus_tag="N7C74_000485"
     CDS             50599..52878
                     /gene="maeB"
                     /locus_tag="N7C74_000485"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HGU2430425.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
BASE COUNT        12837 a        13750 c        13961 g        12417 t
ORIGIN      
        1 gggattctgc tgagcatcta tgaaactgaa tgcaacttat ataaaaatac gtgataaatg
       61 gtgggggctt ccgctgttcc tgccttcttt aatcttgccc attttcgccc acattaatac
      121 tttcgcgcat atttcttccg gtgaggtttt tctcttttat ctgcctctgg cactgatgat
      181 cagcatgatg atgtttttca gctgggcggc attgccaggg atcgccttag ggatttttgt
      241 ccgcaaatat gcagagctgg gtttttacga aacgctctca ttaacggcta attttattat
      301 cattatcatt ctctgttggg gcggttacag ggtctttact ccccggcgta acaacgtttc
      361 acatggtgat acccgtttaa tttcccagcg tatattctgg cagattgtgt ttcctgcaac
      421 gctgtttctg atacttttcc agtttgctgc atttgtagga ttactggcga gcagagaaaa
      481 tctggttggc gtcatgcctt ttaatctcgg gaccttaatc aattatcagg ccttactggt
      541 gggcaatctg attggtgtcc cgctgtgcta cttcatcatt cgggtagtgc gaaatccgtt
      601 ttatttacgt agttattatt cgcaattaaa acagcaggtt gatgtcaaag tcaccaaaaa
      661 agagttcgca ctctggctgc tggcattagg tgctttactg ttgctgttat gcatgccgtt
      721 aaatgaaaaa agcacaattt ttagcaccaa ttataccttg tcattattgc tgcccctgat
      781 gatgtgggga gcgatgcgct atggttataa gctgatttcg ctgctctggg cggtcgtgtt
      841 gatgatcagc atccacagct atcaaaatta cattcccatt tatcctggct ataccacgca
      901 gctgaccata acctcctcca gttatctggt attctctttt attgtcaatt atatggctgt
      961 actggcaacc cgtcagagaa cggtagtcag acgcattcag cgccttgcgt atgtggaccc
     1021 ggtggttcat ctgccaaatg ttcgcgccct gaatcgcgcg ttgcgtgatg ccccctggtc
     1081 tgcgctttgt tatttacgca tccctggcat ggaaatgctg gttaaaaact atggcatcat
     1141 gctgcggatt caatacaagc aaaaactttc tcactggctg tcacccttgc tggaaccagg
     1201 tgaagatgtt tatcagcttt cgggtaacga tctcgcgctg cgactgaata cagaatcgca
     1261 ccaggagcgc attaccgcac tggatagcca tctcaagcaa tttcgtttct tttgggatgg
     1321 catgccgatg caaccgcaga ttggcgtcag ttactgctat gtgcgctcgc cagtgaatca
     1381 tatctacctg ctgctgggag agctaaatac ggtcgccgaa ctttccatcg tgaccaacgc
     1441 cccggaaaat atgcagcgtc gcggggcaat gtatttgcaa cgcgaattga aagataaagt
     1501 cgcgatgatg aatcgactac agcaggcgct ggaacacaac cattttttcc tgatggccca
     1561 gccgattacc ggtatgcgtg gtgatgttta ccatgaaatt cttctgcgca tgaaaggtga
     1621 gaatgatgaa ctaatcggcc ccgatagctt cttgccggtc gcgcacgaat ttggtttatc
     1681 gtcgagtatc gacatgtggg tcattgagca tacgctgcaa tttatggctg aaaacagagc
     1741 gaagatgccc gctcaccgtt ttgctattaa tctgtctcca acctcggtat gtcaggctcg
     1801 ttttcctgtt gaagtcagtc agttgctgga taaatatcag attgaagcgt ggcaacttat
     1861 ttttgaagtc accgaaagta atgctctgac caatgttaag caggcgcaaa tcaccttgca
     1921 gcatcttcag gaattaggct gccagattgc gattgatgat ttcggcaccg gctacgccag
     1981 ctatgcgcgg cttaaaaatg tgaatgccga tctgcttaaa attgacggca gttttatccg
     2041 caatattgtg tcaaatagtc tggattatca gatagtggca tcgatttgcc acctggcgcg
     2101 aatgaagaaa atgcgggtag tggcagagta cgttgaaaac gaagagatcc gcgaggcggt
     2161 gctctctttg gggatcgatt atatgcaggg ttatcttatt ggtaagccgc aaccgttaat
     2221 tgatacgctg aatgaaatcg aacccattcg cgaaagtgcc tgaataatgc gggccgacat
     2281 ttctcgtcgg cccgcaaagc attaagctgc gatttcaggt gtactttctt cttcaatttt
     2341 caaccgccag ccagccacgc cttcccagta ttcttgctcc ttttccagat caagcagtac
     2401 cagcgcattc tgactaaacc agtcatgcgg gaaacgcagt gtccagtgac tgtcatcagt
     2461 aatcagcgtc aatgttggcg gtgtggtggt tgcctgacgc tgattgttga gtaatacgcc
     2521 aaggcgcaat agctgaatca gtggcaggaa ctgtttcttc ttaaacaagg taaagcgcgg
     2581 cagatcgtcg agtttaatcg ctttacggtg atagcgcacc agtgtcgcca tcatcagctg
     2641 ctgttcctga ttaaaacccg gcaagtcgct gttttgtaga atataagcgg agtgacgatg
     2701 caaaccgctg tggttgatat tcaacccgac ctcatgcagc atggcggccc atcgcagtag
     2761 cgcctccagt tgcggatgcg ccagcttcgg ttgctgttcc cgccactgtt cgtacatttg
     2821 catagtggta tccagcaccc gtcgggcctg ttcgctgtcg atgtgatact ggttggcgag
     2881 gctgctggcg gtgcgactac gcacatcctg atgacggaaa cgtccttcca tttcatacag
     2941 tacgccttcg cgtaacgccc cgtcagaaag gcgcagttca cggatggcta aagcatcaaa
     3001 cacaccgcat aaaatcgcta gtccaggaac gaagactgtt ttccgctctt cggaaagacc
     3061 tggtaaactc agcgatgcga aattacggtg ccgtaaaact tcttttacca gtttttccag
     3121 acgttccggg gtaattatcc cgtctttctc gcccatttcc attaacactt catgggcggc
     3181 ttttatggta ccggaagcgc ccattgccac gttccagccc tgaatacgaa attgccaggt
     3241 taaagtttcc agtttttgtg ctgccgccat gcgagcgcgc tgaaaattct ctttattgat
     3301 gaccccgcca gggaaataaa gctgggcaaa gctgacacac cccatccggc ggctttcaac
     3361 gagaatcggt tcgaaatttt caccaatcac cagttctgta gaaccgccgc caatatcaat
     3421 aaccagtttg cgaccttttt ccggttgggt atgttccacg cccataaaaa tcagacgggc
     3481 ttcttcatta ccggaaataa tttcaatcgg gtagggaatg accttttccg cacgtttcag
     3541 aaagtcagtg gcgttcagcg cctgacgcag cgtatgggta ccaactatac agacgctggc
     3601 aggagaaaac ccttgtagcc gttcggcaaa cagcgacaga cagttcaaac cgcgcgtcat
     3661 tgcctcttca ctcaacatat tatctggccc caggccgtcc gccagatgca cccgctgttt
     3721 caggcggcca ataatctgca tggcaccatc taccacacgg gctatgacca tgtgaaaact
     3781 gtttgaacca agatcgaccg cagcaaactc ctgcggacga ggggatttat cgtgtattgg
     3841 catagggtta ttcaggttgt tcgagtgatt tgatgtagtc ataaatcgcc aactgcgccc
     3901 gtactttgcg gcgattgccg cggggaacgt agcgattact gagttcttta tcgatataac
     3961 gtgctttgac cgtatcgctg aacaatatgt cgatgatgtc cagcacccgc tgcttcaggc
     4021 gcggatcgag cagcggcgtc gccacttcaa tacgataatc aatattgcgc gtcatccagt
     4081 cggcggaaga aaggtagacc tttttatcgc cgccattttc aaaaatataa acccggtcat
     4141 gttcaaggta acggtcaaca atactgatgg cacgaatgtt gtcgctaatg ccttccagat
     4201 tggggatcag cgaacacatt ccgcgaacca gcagattaac cggtacgccg gagctggagg
     4261 ccgcatacag acgatcaacc aggcctttat cgacaaggtt atttagcttc agggtgatac
     4321 cactgggcag cccttgctgc gcgttggcga tctcgcggtc caccatttca tacaacaggc
     4381 ggcgggagtt ttgcggcgac accattaaat aatcaaatgt caccggacgg tatgggtttt
     4441 caataaagtt aaatacccgc cgtacttcgt tggtgatgcg cgcatcggcg gtcagcaacg
     4501 aatagtcagt ataaagacgc gcggtttttt cgttaaagtt cccggtcccg atgtgtgcat
     4561 aacgcaccac ttcaccgttt tctttacgtg aaatcagaaa cagtttggcg tgaattttca
     4621 gccccggcgc agagaagata acgtgcacgc ctgcttcggt caggcgcttc gcccagtgaa
     4681 tgttggcttc ttcgtcaaaa cgtgcctgta gctcaaccac cacggtgact ttcttaccgt
     4741 tgtgtgcggc gtggatcatc gagtcgatga tgcgtgaatc ttttgccaca cggtaaatgt
     4801 tgattttaat cgccagcacg ctcggatcga acgaggcctg acgcagcagt tccagcacat
     4861 gctcaaaggt gtgataagga taatagagca acacatcgcg ttcgcgaatg gcatcaaaac
     4921 cattgcggaa ctgggcttta tcaaaccaaa tatggcgtaa acgcggcagt ggtttgttca
     4981 ccagattggc tttgccgaca ttggggaaat taataaagtc tttaaaatta tgataacgac
     5041 cgccggggac gatggagtcg tagcgggaaa tagtcagttt ttcgcgtaac acttcaacca
     5101 gcgcattggg catatcgcgc tgataaacaa aacgcaccgg ctcagcagtt aaacgctgct
     5161 tgagactgga agacatcaac tccatcaggc tggcttccat ctcatgcact aaatcgtatt
     5221 cggcatcgcg ggtcatcttc attgaatagg cattcagcgc gtcataatca aagaagcctt
     5281 tgaaaatatc atcaaggcag taacgcagaa tgttatccag aagaatcatt ggcttgcgtc
     5341 gacgcggcgc ttctggcggt aaattcacaa agcgcggcac tttatctgat gggatctcca
     5401 gaagcgcgta acggatggta tcgccacgga taatttccac cgccagatag gtgtaatcat
     5461 ctttcaggaa ctgcactaag tcagtgtcag gattgattaa aatcggcgta atgtgctgac
     5521 gcagatactg cttaaaataa tgacgcagcc agttttgttg attgacggag agctggcgtt
     5581 cattaatcag gaagatctgg ttgcgcgcca tctccagcaa tagctcgttg tagaggccgt
     5641 cgaattcctg atcggctttc agcacccggg actgaatttt gcccagtaaa tggcgggaat
     5701 gagagttgga gccttgttct tcgctaataa tgatgcgtcg cttcagttca gcgaagcgga
     5761 ctttatagaa ctcatcaagg ttattggaat agatccccag gaaacgcatc ctttcaatca
     5821 gcgggttaga tttgtccgcc gcttcctgaa gcacgcgttc attgaacgat aaccaactga
     5881 gctctttttc gatgtatagc ttttcctgac ccattaccac ttttactccg ttttattcac
     5941 gggacactgc ctggatatta tggcgagcaa tattggcaga tgtccaacta tgccagaaaa
     6001 gggggaaaaa aactgctgga gcttgcgatt tacaggtgaa ttgaaaaacg ccagcggcag
     6061 agctggcgct ttaactacgg ggcattactc gtcggcagcg tagccctgcg gcggcagacg
     6121 ttgaccatcc agccacgcgg cgttttcgtg cattttcaga cgaccatcgg caaaccagct
     6181 aatcaccagt ggataaatgg cgtgttcctg ggtttgcacg cgggcggtga cgtcatcctc
     6241 cgtatcacca gcaaataccg ggactttcgc ctgtaaaata accgggccac cgtccagttc
     6301 atcggtgacg aaatgcaccg atgtaccgtg ctcttcatcg ccattttcca gcgcctgacg
     6361 atggggatgt aatccgggat atttcggcag cagagaaggg tgaatgttca gcaaacgccc
     6421 ggcatagtgg gagacaaatg ccgggctgag aatgcgcata aaaccagcca gcacgaccac
     6481 atcgggtgcg tacatgtcga tttcatgaat caactcccgg tcataggctt cacgactgtc
     6541 aaacgcgctg gcgatgagcg tatgcgttgc aatacccgcc tggcgggcgc gttcaaggcc
     6601 gaacgcgtcg gccttattgc tgaaaactgc ccgtacggtg cctttaattt tgttggtttt
     6661 acaggcgtca ataattgcct gcaaattact tccgttgccg gaaataagca ccacaatatt
     6721 cattattcga taaccacgcg ttgttcggaa tcagaggctt tgatgatacc gattttccac
     6781 gcgttttcac cgttggcatt gagcagggcg agggctttgt ccacttccgg agcaggcagg
     6841 gcaataatca tcccgacgcc gcagttgaag gtgcgataca tttcatggcg ctcaacgtta
     6901 ccagccgttt gcagccagtt gaacacttcc ggccactgcc aggaggattc atcaatcact
     6961 gcctgggtat tatctggcaa tacgcgcgga atgttttccc aaaagccgcc gccggtcagg
     7021 tgcgcaatgg cattaacatc gaccttttca atcaactcca gcactgactt cacgtaaatg
     7081 cgggtcggtg ccagcagatg atcggctaat ggcttaccat caagttcggt ggtttgcgga
     7141 tcacaaccgc tgacttcaag aattttgcgc accagcgaat agccgttcga gtgcggaccg
     7201 ctggaaccga gtgcaatcag cacatcgccg tcgctgactt tagagccgtc gatgatttct
     7261 gatttttcta ccacgcccac gcagaaaccc gcgacatcgt aatcctcacc gtgatacatc
     7321 cccggcattt ctgccgtttc gccacccacc agtgaacagc ccgattgcag acaaccttcc
     7381 gcaatgccgc tgatcaccgc tgaagcggta tcaacatcca gttttccggt tgcgtaatag
     7441 tcgaggaaaa acagcggttc tgcaccttgc accaccaggt cattaacgca catggcgacc
     7501 agatcaatac caatggtgtc gtgacgtttt aagtccattg ccagacgcag cttggtacct
     7561 acgccgtcag tgccggaaac cagcacgggt tcacgatatt tttgcggcaa tgcacacagc
     7621 gcaccgaagc cgcccagacc gcccatcact tccggacgac gcgttttctt cactacgcct
     7681 ttgattcttc caaccagagc attacccgcg tcaatatcaa caccggcatc tttgtagcta
     7741 agagaggttt tatcggtcac tgcttgggtc cccacgcgtt acttgcggta gaaaaataaa
     7801 attcggcgca attctaacag ggaaagcaaa cgtttgcgag actgctttac acaacctttt
     7861 tgcacgtctt ttccccaggc gcgcggcgaa agaagacttg tgccagggca aaggttagtt
     7921 ttcggatgga ataatcttct ttcataacca tttgaatata aaataacttt atctcaaacc
     7981 gttatcattt tgactaaagt caacgaaaat aatattgccg ccttgaagaa aggaggtata
     8041 atccgtcgat tttttttgtg gctgcccctc aaaggagaaa gagtatgaag atcgtggaag
     8101 tcaaacaccc actcgtcaaa cacaagctgg gactgatgcg tgagcaagat atcagcacca
     8161 agcgctttcg cgaactcgct tccgaagtgg gtagcctgct gacttacgaa gcgaccgccg
     8221 acctcgaaac ggaaaaagta actatcgaag gctggaacgg cccggtagaa atcgaccaga
     8281 tcaaaggtaa gaaaattacc gttgtgccaa ttctgcgtgc gggtctgggg atgatggacg
     8341 gtgtgctgga aaacgttccg agcgcgcgca tcagcgttgt cggtatgtac cgtaatgaag
     8401 aaacgctgga gccggtaccg tacttccaga aactggtttc taacatcgat gagcgtatgg
     8461 cgctgatcgt tgacccaatg ctggcaaccg gtggttccgt tattgcgacc atcgacctgc
     8521 tgaaaaaagc gggctgcagc agcatcaaag ttctggtgct ggtagctgcg ccagaaggta
     8581 tcgctgcgct ggaaaaagcg cacccggacg tcgaactgta taccgcatcg attgatcagg
     8641 gactgaacga gcacggatac attattccgg gcctcggcga tgccggtgac aaaatctttg
     8701 gtacgaaata aagaataaaa ataattaaag ccgactttaa gagtcggctt ttttttgagt
     8761 aaagcgccta taacacataa tacagaggat aatactatga cgcgccgtgc tatcggggtg
     8821 agtgaaagac cgccactttt acagacaatc ccgcttagtt tgcaacattt gttcgccatg
     8881 tttggtgcaa ccgtcctggt gcccgtctta tttcatatta acccggcgac tgtactgtta
     8941 tttaacggta ttggaacgct gctgtatctc ttcatctgta aagggaaaat tccggcttat
     9001 cttggttcca gctttgcctt tatttcaccg gtattgttac tgttgccgtt agggtatgaa
     9061 gtcgcgctgg gcggctttat tatgtgcggc gtgctgttct gcctggtttc ttttatcgtg
     9121 aagaaagcgg ggaccggctg gctggacgtg ctgtttccac ctgcggcaat gggcgcaatc
     9181 gttgccgtca tcggtctgga gctggcgggc gtagctgccg gtatggcggg tttactcccg
     9241 gctgaagggc aaacgccaga ctccaaaacc atcatcatct ctattaccac cctggcggtc
     9301 acggttttag gttccgtgct gttccgtggt ttcctggcaa ttatcccgat tttaattggc
     9361 gtgctggtgg ggtacgcgct ctctttcgca atgggaattg tcgataccac gccgattatt
     9421 aatgctcact ggtttgcgct gccaaccctc tatacgccgc gcttcgagtg gtttgccatt
     9481 ctgactattc tgccagcggc gttagtggtt attgccgaac acgtagggca cctggtagta
     9541 acggctaata tcgtcaaaaa agatctgctg cgcgatccag gtctgcaccg ttcgatgttt
     9601 gctaacggct tgtcgaccgt gatctccggc ttctttggct ccacgccaaa caccacctac
     9661 ggggaaaaca ttggcgtgat ggcgatcacc cgtgtttaca gtacctgggt tatcggcggg
     9721 gcggcgattt tcgctatcct gctttcctgc gtcggtaaac tggctgccgc tatccagatg
     9781 atcccattgc cggtgatggg cggcgtttcg ctgctgcttt atggtgtcat cggtgcttcc
     9841 ggtattcgtg ttttgatcga atcgaaagtg gattacaaca aagcacagaa cctgatcctg
     9901 acttccgtga ttttgatcat cggcgtcagt ggcgcgaagg taaacattgg cgcggcggag
     9961 ctgaaaggta tggcgctggc gaccatcgtc ggtatcggct taagtttgat tttcaaactg
    10021 attagtgtgc tgcgccctga agaagtggtg ctggacgcag aagacgcgga tataacagac
    10081 aaataatgta ccgaccgggc agtgttgctg cccggttcaa acatcatggg attctgtggt
    10141 aaacttctcg cgattttgtg aaatcctggt tgaggtatct ctgaacacac cggcacagct
    10201 ctctttgcca ctttatcttc ctgacgacga aacctttgca agtttctggc cgggggataa
    10261 ctcctcttta ctggccgcgc tgcaaaacgt gctgcgtcag gaacatagcg gttacatcta
    10321 tctctgggca cgcgaaggcg cggggcgcag ccatctgctg cacgcggctt gcgcggaatt
    10381 gtcgcagcgt ggcgatgcgg tgggctatgt cccgctggat aaacgcacct ggtttgttcc
    10441 ggaagtgctc gacggtatgg agcatttgtc gctggtctgt atcgacaaca ttgagtgtat
    10501 tgcaggcgat gagttgtggg agatggcgat tttcgatctc tacaatcgaa ttctggaatc
    10561 gggcaaaaca cggttgttga tcaccggcga tcgtccaccg cggcagttga atctgggatt
    10621 accggatctc gcgtcgcgac tcgactgggg gcagatctac aaattgcagc cactttctga
    10681 tgaagataag ttgcaggcgc tacagttacg cgcgcgtttg cgtggttttg aactgccgga
    10741 agatgtgggg cgtttcttgc tgaagcggct cgacagagaa atgcgcacgc tatttatgac
    10801 gttggatcag ttggatcgtg cgtcgattac cgcgcaacgt aagctgacca ttccgtttgt
    10861 gaaagaaatt ctgaagttgt agataaggtg tttattgtcg gatgcggcgc gaacgcctta
    10921 tccgaactac gcgaagttaa tccatagaat tcgtaggccg gataagacgt ggcaagcgtc
    10981 gcatccggca atgtgcttca actagcgatg gagcacatct taacccacga tttccagtac
    11041 ctgctccggt ggacgaccaa tccgcgcttt gccattcgcg accacaatcg ggcgctccat
    11101 cagcttcgga ttatccacca ttgcctgaat cagcgcctct tcgctaagcg aactgtcagc
    11161 caggttcagt tctttataaa gatcctcttt ctggcgcatc aattcacggg cgctattcat
    11221 ccccagcatt ttcagcaaat cgcgcagcgt tgccgcatcg gcgggtgtct caaggtagag
    11281 caccacttct ggctccacgc cgttttcttt cagcaggttc agcgtttccc ggctctttga
    11341 acagcgtggg ttatggtaga tttttacctg tttggtcatt gttcttcctt taatgcgaat
    11401 tacatcttgg tataaggctt aaagcgttcc tgcagctggc gcaactggtc gatgcgcgca
    11461 tcgtaacgcg cttgttgcag gctgcctaat ttcacctgcg aactggcgct actcaacagc
    11521 gaaatggcct gatcgagtcg tccggcgagc gcataacctt ctgctcgcgc agccagctcc
    11581 tgatcgcggt tatttagcgc ggcttccgcc tgtgccagca aatcccagcc gttgctgtca
    11641 tctttattat taaaggtgta gcgattaaga atattggccg cttcttgtgg ttgaccgcct
    11701 tgtagataag cgttcgccag gttgagctgc aacacaggat tggtgcgcaa atcgcgggca
    11761 tttttcagac gattgatcgc ctcattggct ttgttttgcc caagatcgat atcagtagcc
    11821 agatcgagat accatgcgtt gccaggttct gccgccagta acggttgcag cgtttttcgc
    11881 gcctcgtcgt atttattggc ttccatcgcc tgtaaagcac gaccatattg cgccgctcgt
    11941 tgctgacgaa cgtttccttt cgcccattca tccagcaaat cactggtgag ctggttacgt
    12001 ccggaattat acatccccag tgtgcgcgct ttcgccagat agaaatcttc cgacgactgc
    12061 accaccatcg ggcgcatctg attagcacgg ttgcgggcat ctgccagacg actttccggc
    12121 aacgggtgag tcagtaaaat ttccggcggg cgcgaggagt aacgcgcctg atcgagtaat
    12181 ttttcgagga aggttggcat cgcctgcgga tcgaatcccg agcgttgcag cacctgaata
    12241 ccaatgcggt ccgcttcctg ttcattttgc tgggtgaaac tgatcatccc ctgacgcgtt
    12301 cccgccagtg taccggtcag cgccgccatc cccgcctgcg gactggccat cgccagtaaa
    12361 atagaaccta acgcgccgac ccaggtcagc ggcgcgttgc gctgctgatc ttccatcgct
    12421 cgcgccaggt gacgttgggt gacgtgggag atttcgtgcg ccataactga agccagttga
    12481 ctttcgttat cggaataacg gaacagggca gagtgcagca ccacgttgcc gccaaagaaa
    12541 gcaaaggcgt taatttcgtc gttgttgatc agaaagaaat gaaacggtgt cttaaccgaa
    12601 ttggcatgcg aaaccagacg catccccagc gaattaatat attgcgttaa cagcgggtca
    12661 ttaattaacg gcgcgctgcc gcgtagctgg cggacataat agtcgcccat ctgcatttcc
    12721 tgaccaatgg aaagcgtgct tcctgcggag gttcccatat ccggcaaggt gtctgcgctg
    12781 tcggcaaatg ccggggctac ctgaccaatg gtcatagcag caatgagggt tgcaaccagg
    12841 ttttttttca actgcctgaa cataacctct atcctgtatt tctgaagata gtcatttgac
    12901 cgatactgtg aaatatcgtt cccaccgcgc cgcctttgag tgtaacggcg aagattagcc
    12961 cactcaaatg acttttcagt ttcctgaatc cgtcataaaa agcgaagtct tttttgtgac
    13021 atttcgatac aattgcggat cgcaatcccg ctcttgaggt tcagggaagg tctttatgct
    13081 cgaaatgttg atgcaatggt atcgccgccg ttttagcgac ccggaagcga ttgccttgct
    13141 ggttatttta gttgccggat ttggcattat ctttttcttt agtggcctgc ttgctccgtt
    13201 gctggtggct attgtgctgg cctatttgct ggaatggcca accgtgcgcc tgcaatctat
    13261 tggctgctcc cgccgctggg cgacgtcgat tgtattggtg gttttcgtcg gtatattgct
    13321 actgatggcg ttcgtggtac tgcctatcgc ctggcagcag ggcatctact taatccgcga
    13381 tatgccgggg atgctcaata agctttctga ctttgccgcc acgttgccgc gccgctatcc
    13441 ggcgttaatg gatgcgggca ttattgatgc aatggccgaa aatatgcgca gtcggatgct
    13501 gaccatgggc gattcggtgg tgaaaatttc cctcgcctcg ctggtcggtt tgctgaccat
    13561 tgccgtctat ctggtgctgg taccattgat ggtcttcttc ctgctgaaag acaaagagca
    13621 gatgctgaat gccgttcgcc gggtgctgcc gcgtaaccgt ggactggcag gacaggtgtg
    13681 gaaggagatg aatcaacaaa tcaccaacta tatccgcggc aaagtgctgg agatgatcgt
    13741 agtagggatc gccacctggc tgggattctt actcttcggg ctgaactatt cgctgctgct
    13801 ggcggtgctg gtcggcttct cggtacttat tccgtacatt ggcgcatttg tggtgaccat
    13861 tccggtggtt ggcgtggcgc tattccagtt tggtgcaggc actgaattct ggagctgttt
    13921 cgcggtgtat ctgatcattc aggcgcttga cggcaacctg ttagtaccgg tgttgttctc
    13981 cgaagcggtt aacctgcatc cgctggtgat tattttatcg gtggtgatct tcggtggttt
    14041 gtggggattc tggggcgtat tcttcgccat tccattggcg acgctgatca aagccgtgat
    14101 ccacgcctgg cccgatgggc aaatagcgca agaataatct tcagcccgac gaactgtttc
    14161 gccgggctga tttttcagga ttcctgacgt aaataaatag cccgataaca aatactcacc
    14221 agcactgcac cgccgataat attacccagc atcaccggga gcagattggc ggtaataaaa
    14281 tggctgaccg tcagtgcagg aaaattgtct gcgctactgt gcgccagttg ccagaaggag
    14341 gtaggggcga aatggcgaat ggcaatggcg aacggaatca caaacaaatt ggcgatacag
    14401 tgctcaaagc cactggcgac aaacagggtg atgggcaaaa tcatggcgac gattttgtcg
    14461 cataacgaac gcccgcaata actcatccac agcgccaggc aaaccatcag attgcacata
    14521 atgccgaggc tgacagattc agtaaatgta tgatgcattt tgccctcggc gcagtgtaaa
    14581 accgcgactc cccacatcgc gttttcactc atcaccagcc cggaaaacca gattaacaaa
    14641 ctgaaacagg caatacctgc cagattaccg caggccacca gaagtgcgtt aatcagccaa
    14701 gttcgccaac taataacgcc ccgacttttt gccatcaccg tcattaccga cgaggtgaac
    14761 aggctggtgc cgcaaaccgc cagcaaaata aagccgagtg taaagcaaag gccgcccacc
    14821 agatgggtta atgcctgtga agacggggca tcggcgataa cggaaaggta aaaagtaaat
    14881 ccgatcgcca taaacacgcc agcggacatc gccattaaaa agaccgacac tttactgcgg
    14941 gcaattttat gggcggcaat ccgttcagcg attgccgctt ttctggcgct caactgcaag
    15001 tcgaaagaga gtttgtttct catgcatact cctgcaaaag cagattacaa cacctcgcga
    15061 accgagatcc ccagacgctg cattcgtgac agcagcgtgg tgcgcttcat ccctaatcgt
    15121 gtcgccgcgc cacggggccc ggcaacaatg ccattggttt ctcgcaatac ctgaacaatg
    15181 cgctgacgtt cttcttcgtc attctctggc gtcgtcgggt gcagcaactg cgccattgaa
    15241 ctgcgaagcg ctgaatcttc atttagcgtc ggcagtaaac ggctttgtcg gacattaaga
    15301 tgtaaattca gactgttacc gcgagtcagc agtaccgctc gctcaatcac gttttccagc
    15361 tcgcgcacgt tgcccggcca atcccacgac atcaactggc gtagtgcctc ggtcgggatg
    15421 gcgtcaattg cgcgattcat atggcgcgcc attttttgcg tgaaatgttt tgctaaaaga
    15481 gggatatctt ccggacggtc tcgcagcggc ggcaattcca gtgggaagac attcaggcga
    15541 taaaagagat cgctgcgaaa ctggcgatct tcaaccattt gccacaaatc acggttggtg
    15601 gcggcaatga ctctgacatt caccgggatc gttctactcc cgccgagacg ctcaatttcc
    15661 cgctcctgca atacgcgcag caatttaggc tgaagttcta acggcagatc gccaatttca
    15721 tcgagaaaca acgtaccgcc atcggcaatt tcaaaacgac cacgatgggt attaatcgca
    15781 ccagtaaagg cccctttgtc atgaccgaat aactcacttt ccagaagact ggcggggatg
    15841 gcagcgcagt tgattttgac cagcggcttg tcgcgtcgcg ggctaagttg atggatcgct
    15901 ctggcgatca cctctttgcc ggttccggtt tcaccacaaa tcaacaccgt actgtcgctc
    15961 ttcgccacaa tatctacctg ctggagcagg tcttccattg cctggctttg atagataatg
    16021 tcaccgacgc ccagattcga aaggagctgc tcgctaagct ggtggttttc ttgctgcaaa
    16081 ctttcctgca aatcggtcat gctacgccag gcatcggcat tgccaacggc aatagcgatg
    16141 cgatcggcta tgtgttgtag tagctggcag ttttcctcac taaagagagt ggtagaggta
    16201 tgcgccagca acaatgcacc cggtgtatgg ttgccaaagg taagcggtat caggagcgaa
    16261 gattcgcagc cgttagatgc aagtaagtgt aataacgggt cgcgttgcca gagaaacaga
    16321 tcgtctgcac ggtgcgtcaa ggtcggttgt cgggtttgta gcgtttgtgt cagcaataca
    16381 ctgtcgccag gcatattgcg tggcagacac gcacaatgtg aggcagaaag atcgctgcac
    16441 cacaggctga acttctcgtt ctttcgatga tcgcccagta ccatactgac tgaagccaga
    16501 ccgaaaaaat gatggatctc acgagcgacg tcagcgatca gatcgtcgag atcaagatgt
    16561 gacagcactg cattagtgat atccaccagt acccgatgat gatcgcgatc tttctccagc
    16621 aaacgcagcg ccacctgttg ttcttcatca ttgagccatg attgcagaac cggggtgata
    16681 atgcgtgcca gccagttaaa cagtgtgcaa tcctggggag taaaacgact gccgtgggtg
    16741 cgaataaagc gtagctccgc cattccacta ctgccgagaa ggacggcgtg acgcgaataa
    16801 tcgaccatct cgccctgata ctctgttgtt gccgtcacat cgctgccatc atcatggtag
    16861 cggtaataga agttgctttt gagaacatgc agttcaataa gttgtaccgg gctgaacggc
    16921 ggcggcagcg ggatgaaggc gcgtaataaa gacgccttgc cgtgtttctg cgctaaccgc
    16981 tctgcgagca ttccgtttac cgcttcaatt gttattcctt gcggcggggc aaacatcgcc
    17041 tcgtctgaca tagccatcaa cagtccttat taatgagaaa tccttctcac gcaatttttc
    17101 tggccatcag atagatagcc ggttccagca caatttcatc caacagatga atgaattgaa
    17161 tgctacgcgc ttgccacaca gacgtcgcgt catctgccag tggcgcgaat gcccgggtca
    17221 acagggcggt gtggctggcg tcaataacaa tctcaccaaa agtcatcacc cccagcatct
    17281 tacgtcgggc ttgctcctct tcgaccagtg caagccaatc ttcgtattcc gtcagtgggc
    17341 agcggaaggt gacattcaga caatcaatca cgccaacgtg atgaccgata gccagcgagt
    17401 aatacattac ctgggagctg tgttccggca tctcgtcgct actggcgaca aacttttttc
    17461 gcagcgtcca gaaaacaatt tccccgcact cttcagtcat gtaacccccg gagtacgcga
    17521 aagagttgct gaacgatact attgagacgt ggatcgtcgg catcgcgcaa ccaggtgtta
    17581 acccgatttc ctgtaggatc gttgcttaaa tggcgcagga gccgatcgca aatttcttgc
    17641 ccctgacgat agcccgccag ccgccgcgct tctcgctcaa tggcgatacg ctgtgatggc
    17701 gggatctgcg gccacaacgg ttgcatagtc accccggtgg gatcgcgata atccaccgcg
    17761 tgaatcttct gttgcagcaa accgagcgcc acggcgaaac cgtgaatggt ggcggccggt
    17821 gttggcgggc agccggggat ccaaacatca atggggacaa tggtgtcgct gccgccccag
    17881 acgctgtaga gatcgtggaa aataccgccg ccgacaccgc acgcgccgta ggaaacacag
    17941 attttatgat cgggggcaga ctcatacgcc cgaagtgcag gcatacgcat cgcccgggtg
    18001 actgcgccag taaataacaa aatatcggcg tgacgcggtg atgaaacaac cttaatgcca
    18061 aaacgttctg cgtcgaatac gggtgtgatg gcggcaaaaa tttcgatttc acaggcgttg
    18121 cagccgccgc aatcgacgcg ataaacgtag gctgagcgac ggatatcctg tagcaaatgc
    18181 cgcttcatct tttgcgtttg ctcgtccagc gtgatgggct ggctgacatg ttgtgtaagc
    18241 actggactca tagctgctcc ttagccacca gcggtacatc tgtatcgtcg ttgatcagcg
    18301 tcgcgcgttg tttgcactcc gggcagacgc tcgcttgcgc ccgcaacatt tcgcggtttt
    18361 gtggcgcatt ttgttgctgt gccaacaatt cagcagccag tgcgacggtt ttttgcgggg
    18421 caaacgggcg ttcgcaacgg ctgcaacgtt gtagatggaa cgtcgcgcgg gtatagagat
    18481 cggctttatt ggtgacggtc agttcaaagt tattggtaag ctggatggct ctggtcgggc
    18541 acacttcttc acaacgtccg cagtaaatac aacgccccag atagagctgc caggtgcgcg
    18601 aattttgctg gtcgtcggtc tggatagtca gcgcatttgt cggacaagca caggcgcagg
    18661 caccacaggc aatacattgg ctgggcatca ggtccggttt cccgcgaaag cctgggctga
    18721 cctccagtgg cgcgaaggga tatttcaccg tcgcggttcc ggcgcgcata atagttttca
    18781 gtaacttcag catcttctgt ccttacttca gcggcgaacg gttacgatca atgccgtagc
    18841 gttcgatctc tttatacggc acggtttttg actggcgctt gcgcacatca accagcgtca
    18901 cacggtcggt acaggagtag cagggatcaa ggctaccgat aatcagcggt gcgtcagaaa
    18961 cggtattgcc gcgcagcatg taacgcaaca ccggccagtt ggcgtaggtg gcggcacggc
    19021 agcgccagcg gaacaatttt tggttatcac cgagcatgct ccagtgcacg tcttcaccgc
    19081 gtggcgcttc aacaaagccg agcgcgaatg cgtgaggttt atagctaaag ccttcggtca
    19141 gcagtggggt atccggcatg ttgtcgagag caaattccag cattgccagc gaatcaaacg
    19201 tctctttgac acggaccatc acgcgggaga aaacatcgcc gccggtgaag gtaaacagtg
    19261 tttttggaat attgccgtag tcggcgtagg ggtgatcaaa gcgcaaatca cgggcaaaac
    19321 cactgccgcg gatcagcggc cctacagggc tataatcacg ggcgatttgt cggtcgagaa
    19381 tgccaatgcc ctgagtgcgt tgttccatat tcggcgtggc aagcagcatt tctaccagct
    19441 ccgacacgtc ggcgcgcatc tcgcgcacca gtttcagcgt ttgcagacgt tgctctttga
    19501 gaatatcgcg gcgaacacca ccaatcagat tcagaccgta ggttttacgc gacccggtca
    19561 gcaattccgc cattgtcatc gacttttccc gcacgcggaa aaattgcata aagccggtat
    19621 caaatccaac aaaatggcag gagaggccaa ggttgagcaa atgactgtgt agccgttcga
    19681 cttccagcag aatcgagcga atggtatgcg ctcgttgcgg cacctcaatc cccagtgcat
    19741 tttcaaccga gttggtatag gccacactgt gggcaaaacc gcaaatccca cacacgcggt
    19801 ccgataagaa ggtcacttcg ttgtagccca tccgcgtttc tgccagtttc tccatgccgc
    19861 gatggacata aaacagacgg taatcagcat cgacaatttg ctcgccatcc acaaacaagc
    19921 ggaagtgacc cggttcatcg gaggtgatat gcagcgggcc gacagggatc acccgcgcac
    19981 cgctgttgcc ctcattgata aacggatacg tttcggaatc agtcgtcggt tcagggcgca
    20041 gtcgataatc catcgcatct ttgcgcagcg gatgcatatc ttccggccag tcatcgggca
    20101 acaccaggcg acgctgatcc ggcaggccaa ccggaatcag cccgtacata tcacgaattt
    20161 ctcgctcgcc ccagaccgcg gcagggacgc gcggtgtgac tgacggaaac tcccgactgt
    20221 cggcatcgac cagcgccttc accacaatcc agcatttttc ggccccttcc attgaaaggg
    20281 cataataaac cgcgtaatga ccgttaagtg tccgctcgtc gttgccaaac aggaccggaa
    20341 gccagccatc atgttgataa taaagatact gtacaacgtc aggcagcaga ttgattttca
    20401 ccgtaatggt gacctgttca ggcgtttgtc gctcttcatc cagcaccgcg ccggggaact
    20461 gcgttttcag ggcggcgaga atcgcttcgc cacgatttga cgatgaatta acgttcactg
    20521 tgtttctccg aaacagatgc ggtgcctgaa ggcaaaaaat catgccaggt gctacgttgt
    20581 gcaggcaggt cgtgcgtccc tgagaggact atagtggaag cgcccgccag gatcctgatg
    20641 acaggttgtg gaatatgcgt tcccatcgcc agcatcatga ccagcagaat caccattggc
    20701 gaggtggtca accagccgag atcaccccgg ttaacggcct gcggcggttt cgccattaac
    20761 acccgcgcag ccatccgtac caggcccgcc agcaccagcg ttaacagcaa taacagcagg
    20821 acgataatca gcaggtgatt acgtgccagt ccggcggtaa cggtcataaa ttcgctaaga
    20881 aaaatgttga agggcggcat ccctgccagc gccagcgcac cgccgccaaa cagcacggcg
    20941 gtaaatggca tgattttgag catcccacag acgacgttga gatcgcgcgt gccgtacttg
    21001 agcagtacat tgccggaacc gcagaacagc agcgtttttg ccagactgtg gtttaaggtg
    21061 tgcagcagcg cggcaaaaat tcccagcggc ccgccaatgc ctagcgccac cgcgaccagt
    21121 cccatgttct ccacgctgga gtacgccagc agacgcttaa tgtcccgctg taccagaatg
    21181 aaaaatgccg ccacggcaac cgacaacatg ccgaagatga gcaacaaccg gttggggaaa
    21241 tcgctgccga tggcctggca aataatgatg taatagcgaa tcagcaccaa cagcgcgcag
    21301 ttcagcaata cggcggagag tagggcgctg accggactcg gcgcttcact gtgagcatcc
    21361 ggcagccagg cgtgcatggg aaatagcccg gttttggtac caaagccaat tagcacaaac
    21421 acaaaggcca acagcattaa tgttggatca agcaaggacg attgcttaag aacctcgctc
    21481 cagaatatcg ccatttctgc ctgcggcata acgctggcgg cgttggcgta tactagcacg
    21541 gtaccgaaca gaccaaaagc gacaccaaca gtacaaataa tgatgtactt ccatgctgct
    21601 tccagcgatg aacgctgacc gtaaatgcct accagaaacg ccgagcttaa ggtggtggct
    21661 tcgatcgccg cccacatcac aatcaggtta ttgctggtaa caaccagcag catggtgaac
    21721 aaaaacagat ggaagaaacc gtagtaatcg cacagcgtaa cgggtgaaag ctcgccgtgc
    21781 tcaacttcat gacgcatgta gccaatcgag taaacaccgg tgagaaagcc aatcacacca
    21841 agaatggcga ggaacaaacc gcccagacca tcaatatgaa gccacagtcc cgcagcgaat
    21901 atttctcctg catcagcggc agtttggacc acccagagtg ccagaatcag cagcagtgtg
    21961 atccctaagc tatgtaatac tgtcaccgtg cgagtcgcag agtgccctcg tttccggcag
    22021 gcaaaacaga gcagcgaaaa aagcagcgga gtgagcagga gtaaagcgaa catcacagaa
    22081 taactcatct cattatccct tcagcgcggt caagttgttc acgtccagcg tgccgtgggt
    22141 acgccatatt tttcttgcca gtaacaccat cacaatgacg gcgaagatgg cgtcggtagc
    22201 gataccaatt tccaccagtt ccggtgcacg ccaggctaga agcgccagca ccagatggga
    22261 gccgttttcc atcaggcagt aaccaaaaat ttgccgcagg atattgcgct ggctaacaat
    22321 gcacagcagg ccaagcagaa aatgacctaa cgccaccgcc agcgctggtt tcagcccggt
    22381 agccatcgat agcttcacgg gctgaacgac aaatgcgcaa agcaggacaa ttaacgccgc
    22441 gagcagtgcc atcattgccg gaccgaataa cgctttttcc gggatgttct ggggaatatt
    22501 tcgtgcagcg taagtcatga ttaacggtac cagcagcact ttggtgataa aggcgctggc
    22561 ggaccagatc agcagttgct ctgcggcgaa caggcacgag agggtggcga aaatagacac
    22621 cagcaccagc gactggcagg cgtaaaatcc gcatgacagg cgatagcttt tgacgctaat
    22681 cacaaacagc gatgtcagca tcatcagtcc cgccagatta tttacgatca tagaaccggt
    22741 cattcatcat ctccttactg caaccaggtc gcggcgatta cgctggaaat cagcgacatc
    22801 acaatcagta caatcagcac caggcgcatt gaccctggca gcggtgcggc atcggcgacg
    22861 gcctcgctcg gtttgccggg gacgacgcga ccaaaccagc gaataaacca ggcgaaactg
    22921 gcgaccgatt caatcatcag cagaatcatg gcgggcagca ggatccagta ctccactgac
    22981 aacgcaaaac cggcagcaaa cagcgggaat ttactgaaga agccgttgaa cggcggtacg
    23041 ccggtaatcg ccagcgctgc cacgcagaaa cccacgcctg gcaacggcag ggtgtgcaat
    23101 acgccacgca gacgcggcaa caagcgcgtg ccgcagctgt aactcagcgc acctgctaca
    23161 aggaaaaaca ggcttttagc gaacgcgtgg ttgacgatgt aggcgatgct gccctccagc
    23221 gccagccgcg agccgaagat ggagagcgac aagccgaaga acatccagcc aagttgagtg
    23281 atggtcgacc aggccagcaa ccgcttcata tcctgctgtg gcaaatacat cagaaatcca
    23341 taaagaatgg tgaccagggc catgaccatg ccaacgccgc caatcacatg cgggatattg
    23401 ccgccgtcga taatggcgcg ggcaaaaatg taaacgccca ctttcaccat cgatgcggcg
    23461 tggagatagg cgctgatcgg tgttggcgct tccattgcgt ccggtagcca cgcttgcatc
    23521 ggtagctggg ccgatttccc ccacgcggca aacaggatgc cgccataaac cagataacgt
    23581 gcgtcgccgt gtaactcgct catcgcgcta agcgcaaacg ttccggtctg caaaaacagc
    23641 gtggcggcgg caagatacaa ccccaacgaa ccgatatggg tgataagcaa cgcttttagt
    23701 gctgaacgct gcgctttatc gctctgataa taactgatca acacccagga gcagccgccc
    23761 gtaatttcaa aaaacaacaa ctgaccgagc agcgtcgagg agagtaccag tcccgccatc
    23821 gcgccgataa acaccagcaa aaatgcgtaa taacgattcg tgccgttatg cgggtgttcg
    23881 cgatttttat ccgtcagata acccgtggag tagatcgtga ccagcaaacc gagaaacacc
    23941 accacaaaca gaatcagcgt acacacgcga tcaatgacca ggccaaacaa tgccacatcc
    24001 cccacgttga ccaacgtcaa cgtgacggca actttatcgg cctgataaaa cgcggagatc
    24061 agcgacaaca tgcacagcgt ggtcagcgcg gcgaacaaaa ccccccattc ggcggcccga
    24121 cgttgtggcg aaaacgaaac gaccagtgcg ccgataaaag gcagcaataa cgtcgtcaga
    24181 gcaagatttt ccatattccg tcagtgctcc ttacagaccg gttaaccaga agacccatgc
    24241 aagcgcagca aggctgaagc caagccaggt cacatggtga atgagtaaaa aacgcccgcg
    24301 tgccagcgtg ttttctgcga ttgaggccag tacaaaaatc agcaaaacct tgagcagcgt
    24361 aacgacaagt gaagtcagca ggcaggcgag agaaagttct tgcgcgcacc caaagggcag
    24421 aaacagggcc acaaacagtg ctgccatcac gacctgtttc agccccagcc cccatttcgc
    24481 cagcgccagc ccggcaccgg aatattctgt cagcgggcct tcctgtaatt cctgttctgc
    24541 ttcagcaaca tcaaagggaa tttttcccat ctcaatgaag caggcaaaac cacaggccag
    24601 taacgccagt acggtggtta gcggcgagtt ccagcccgtt gccagcgtat tgctgatcat
    24661 ctcgatatgc gtggaacctg ctatcagcgc caataccagc agtgagagaa tgagcattgg
    24721 ttcgaccaga atgccgagcg tcaactcgcg actggcaccg actcccgcaa acgggcttcc
    24781 ggtatccagc ccggaaagag caaagaaaaa acgaaacagg gcaagaagat agataagggt
    24841 gatcagatcg ccgccgcccg caaaagggga aacggtaata aatagtggta gggccatcgc
    24901 cagcaccagc atgctgctga ttaataccca cggcatcagg cggaacatca gacctgaaga
    24961 tataggcgca acttcctggc gtttaaacag tttgtggata tcgcgataat cctgccagat
    25021 ccccggcccg cggcgggagt gcatacgcgc gcgtatctgc cgggaaatac ccgtaaaaag
    25081 cggggttagc atcagcagaa tcacggcctg tgctaacgca aaaatgacca gatatccgtc
    25141 gcaaagagtt tgtctcatgg tgatttcctt agacggcaat agcgattagc agcacaacca
    25201 gtgcggcgac cacatacagg caatagagac gaaagtcgcc gctttgcaga tgctgtattt
    25261 ctttggccag ccgttgggtg gcgctcacga tggggcggat cacccgctca tcccagaagg
    25321 gttctgtgct ctgagccctg gcggtgacgt gcgcaagacc tttatttagc ctcagcgtag
    25381 ggtcgagctg ttgtcgtaga cgaaatagcg cagagaagac cacacgcagc ggctgcatca
    25441 cgccattgcc tgacggggcc atcgcatttt cccagccgta gccgcatgcc caggcatctc
    25501 ctgtgcgacg aaacgctgcg cggcgcgaac gacaccacag ccagtaaagg ccagacagca
    25561 aaggcatcgc cagtagtaac agcaacagta atgagggggt gacctgtgta tgaaacgtgc
    25621 cgggtacaag tgctattccg ctggcgacag tgacgggagg ggtattggta aacgcatggg
    25681 caatatgcat tatcttcggt gccagccaac tggcactaag cgcaattaat acgcagagtg
    25741 ccgcgagcag tagcatggcg acgatcatcg tatttggcac ttcctgtgcc tcttcagcgt
    25801 gtgtactgcg cggcgcacca cagaaagtaa taccgtacat tttgacgaag cacattactg
    25861 ccagcccacc agtgactgcc agcatcacca tagcaatagg acccgcaagt tgtagcgcta
    25921 cggcttccac acgacttagt gagaacagcg actgccaggt gtaccattcg ctgataaaac
    25981 cattcagcgg aggaagggct gatatcgcga ggcaaccaat caggcatgct gcggctgtcc
    26041 acggcatccg tttcgccagt gcccccattt tttccatgtc gtgggtatgc aaacgcgaaa
    26101 taatcgcgcc cgcgccgaga aatagcagcc ctttgaacag cgcatggttg agcagatgaa
    26161 acagtgcgcc gagcagtcca accacggtga gcagcgggtc gtgtagtgac agaccgacca
    26221 tcgccacacc gactgccagc aaaataatgc cgacgttttc gacggtactc caggccagca
    26281 gccgtttgat atcctgttcc gccagcgcat acagcacgcc caggagcgcg gagattgcgc
    26341 cgatcgccat caccagaatg ccccaccaca gcggcaaacc cgtttgcgcc agcagatcca
    26401 tcgccacttt caggatgccg aaaataccga ttttgaccat tacgccagac atcaacgccg
    26461 aagcgtgcga tggtgcggca gggtgagcgc gcggcaacca gctgtgcaac ggcatcatcc
    26521 cggctttcgc gccaaaaccg aaaaaggcca gcaggaacac cgccgacgcc agccccggag
    26581 aaagtgaaag cgtgcggaaa ctggcaaaat ctaggctgcc gctttcgcgc cccatcagca
    26641 agaaggcgat cataatcagc accgatccgg cgtgggcgat aaaaaagtag agcatgccag
    26701 cgttgatcga cgttttatcc tgcctggcaa tgaccagaaa ccaggaagac agcgacatca
    26761 tttcaaacag cacgatgaac caaaaagcgt tgtccatcac cagcagggca accatcgatg
    26821 cgatgaaaat attcataaag aagcccatcg ccgccgcgcc tttgccctcg tattcgcgca
    26881 tataagtcaa tgaatagagc gaacaaacca ccaccagcaa ggagataacc agcaccataa
    26941 atgccgagag gctatccatt cgcagggtta aatcggcaaa gtcaaaaggg gtggcaaaac
    27001 gggcaactaa agttacgcct tcatgtaatt gcgtaattcc gctgatgatg ccaatcacgc
    27061 cgcccaccag cgatgtgatg ccggaaagct taatagccag tctgtccaga ccgagtaaaa
    27121 acagcgaagc cagactggca aacagataga ggatcagcga ccaggttaat aattgcaggg
    27181 catccattag cgttgctcct gagtgaggga aaggggatcc tccctgccat tggcaaacca
    27241 gcttgcggca aggcgctgtt tttctttcat ctgacgttgc aggctatcgt cggtgatcag
    27301 ccgtaacgcc tgattcgggc aagcgcgaac gcaggcagga ccttctggca agaaatcaca
    27361 caggtcgcat ttcaccgcga cggtctggac acccggttcc cagcgcagca aagcatgttg
    27421 tgttggcgcg ttttcttcgc cgtctttgag tgagccttca gcctgaaaaa catattgcgc
    27481 aggggcattc accggacggc ttcctgaagc gctgattgcg ccaaatgggc agaccacggc
    27541 gcaaagcttg cagccaatac agaggctttc gttgagttgg atcgcatcat ccctctggga
    27601 gatggcattg accgggcaga cctgcacgca aggggcttcc tcacagtgat gacacacgac
    27661 aggggcagtg agtgttgacg tcttcgccag ggccaggcgc gggtgttgct gtaaaccttg
    27721 cgttttatgc acgtccgaac aggcagcgag acaggtatta catcctgtac accacagtgg
    27781 ttcggccacc acaaagcggt tcatgtcggc tccttgaaaa tgatatgaaa cattgcattt
    27841 cataatgcat aaaggatgcc agtatgatta atttaatata ttcaataagt tatgaattaa
    27901 ctgaattctg aacggtacaa aaatgatggt cattaacgtt cagcaaaaaa tggattcgac
    27961 acccgtgtcg caggggaaag tgagaatgtg atcttgatca cgataagtga ggtgtgctga
    28021 ccgcaagcgc agccagcacg gaatggagca aagtaatcag gcgtgttctt tcagccagtt
    28081 cagcacaacg tcgtggtgat tgctggtttt gaaatcgtca aagacatgtt cgattttgcc
    28141 atcagcgtca atcaggaagc tgatgcgatg aataccatcg taggttttgc ccatgaagga
    28201 cttttccccc cagacgccga attgctcgca cacctggtgg tcctcatcag acaggagcgt
    28261 aaagttaagc agctcttttt ccgcaaaacg ggagagtttt tcgggtttat cggtgctgat
    28321 acccagcaca tcaacgcccg cttttttcaa ctcatccatg ttatcgcgta agccgcaggc
    28381 ctgtacggta cagccggggg tcatggcttt cgggtagaaa taaaccagaa cacgctgtcc
    28441 ctggaagtcg gtcaaattaa cttgttctcc gtcttgatcc ggcaagctaa atttcggtgc
    28501 gatatcaccg gctttcagtg gattcattac ttaactccat cctgttcatc atgttgggaa
    28561 taattgacga cgttaatact gccttgtgca ttgagttctg tacatagggc tttgaacgct
    28621 tgctcaatat ttgctgcgtc cgcagatgcg gggctgtggg cagtaatctg aatatgcaac
    28681 tgcgcagccc gttcattttc agcaggttgt gtgcgcgaca ccagctccgc aatgttcata
    28741 tgatgcgcgt cgaaaagtgc tgtgaagcgt tcaattaaat gcggggagtc tgccacatcg
    28801 acctgaaccc agacagatgc tggcattggc ggacgcggac gcgccgtcgt gcgcttcatc
    28861 acgattaaaa gatccagttc ggcacctttc aacggtaacg ttgattcaat cagagtaatg
    28921 gcattccatg aaccggaaag cagcatgata aacgtgaact cttctcccag catcgccagg
    28981 cgactgtctt caatattaca gccgcaacta ctgacatgac gggtgatggt gttcacaatt
    29041 ccagggcgat cggcacccaa cgcagtgatc accagataat gttgcgatga cagtgtcaaa
    29101 ctggttattc ctttaagggg tgagttgttc ttaaggaaag cataaaaaaa acatgcatac
    29161 aacaatcaga acggttctgt ctgcttgctt ttaatgccat accaaacgta ccattgagac
    29221 acttgtttgc acagaggatg gcccatgttc acgggaagta ttgtcgcgat tgttactccg
    29281 atggatgaaa aaggtaatgt ctgtcgggct agcttgaaaa aactgattga ttatcatgtc
    29341 gccagcggta cttcggcgat cgtttctgtt ggcaccactg gcgagtccgc taccttaaat
    29401 catgacgaac atgctgatgt ggtgatgatg acgctggagc tggctgacgg gcgcattccg
    29461 gtgattgccg ggaccggtgc taacgctact gcggaagcca ttagcctgac gcagcgcttc
    29521 aatgacagtg gtatcgtcgg ctgcctgacg gtaacccctt actacaatcg tccgtcgcaa
    29581 gaaggtttgt atcagcattt caaagccatc gctgagcata ctgacctgcc gcaaattctg
    29641 tataatgtgc cgtcccgtac tggctgcgat ctgctcccgg aaacggtggg ccgtctggcg
    29701 aaagtaaaaa atattatcgg aatcaaagag gcaacaggga acttaacgcg tgtaaaccag
    29761 atcaaagagc tggtttcaga tgattttgtt ctgctgagcg gcgatgatgc gagcgcgctg
    29821 gacttcatgc aattaggcgg tcatggggtt atttccgtta cggctaacgt cgcagcgcgt
    29881 gatatggccc agatgtgcaa actggcagca gaagggcatt ttgccgaggc acgcgttatt
    29941 aatcagcgtc tgatgccatt acacaacaaa ctatttgtcg aacccaatcc aatcccggtg
    30001 aaatgggcat gtaaggaact gggtcttgtg gcgaccgata cgctgcgcct gccaatgaca
    30061 ccaatcaccg acagtggtcg tgagacggtc agagcggcgc ttaagcatgc cggtttgctg
    30121 taaagtttag ggagatttga tggcttactc tgttcataag tcgcgcctgg caacggttgc
    30181 gggtgtttcg cttgttttat tactcgctgc ctgtagttct gactcacgct ataagcgtca
    30241 ggtcagtggt gatgaagcct acctggaagc ggcaccgctt gcggagcttc atgccccggc
    30301 tggaatgatt ttgccggtga cctccggtga ttatgcaatc ccggtgacca acggtagtgg
    30361 tgctgtcggt aaggcgctgg acattcgtcc accagcccag ccgctggcac tggtttctgg
    30421 cgcgcgtacc cagttcacgg gcgataccgc ttcattgctg gtggaaaatg gtcgtggcaa
    30481 tactctgtgg ccgcaggtgg ttagcgtgct gcaggcgaaa aactacacca tcacccaacg
    30541 tgatgatgct ggtcagacac tgaccaccga ttgggtacaa tggaaccgtc tggacgaaga
    30601 cgagcagtat cgtggtcgtt atcaaatctc tgttaagccg cagggttatc agcaggcggt
    30661 tacggttaaa ctgctgaacc tggaacaggc gggcaaaccg gttgcagacg cggcttccat
    30721 gcagcgttac agcacggaga tgatgaacgt tatttccgct ggtctggata aatctgccac
    30781 tgacgccgcg aacgctgcgc aaaatcgtgc ctccaccact atggacgtac aaagtgcagc
    30841 tgatgacacc ggtttaccaa tgctggtcgt acgcgggccg ttcaatgtgg tttggcaacg
    30901 tctgccagcg gcgctggaaa aagtgggcat gaaagtgacc gacagcaccc gttcgcaggg
    30961 caacatggcc gtaacttata agccgctgtc tgacagcgac tggcaggaac tgggcgcgag
    31021 cgatccaggc ctggcatctg gtgactataa actgcaggtt ggcgatttag ataaccgcag
    31081 cagcctacag ttcatcgatc cgaaaggtca tactctgact cagagtcaga acgacgcgct
    31141 ggtagctgtc ttccaggctg cgtttagcaa gtaaaaatac agggctggaa tcatccggcc
    31201 cttttttctg atatgatacg caaacgtgtg cgtcggcagg aaaacgcgat tttagcggta
    31261 attcgcacga aatttgtttg tcggacgtag ttcggataag gcgttcacgc cgcatccgac
    31321 aaaacatccg gcacaccaga cagcaaaaga ttttaaaacg ttaattcaca cccaggagtg
    31381 ataaagatgc aaaagcaagc tgagttgtat cgtggtaaag cgaaaaccgt atacagcacg
    31441 gaaaacccgg acctgttggt gctcgaattc cgcaatgata cgtcagcagg ggatggcgcg
    31501 cgcattgagc agtttgatcg caaaggtatg gtgaacaaca agttcaacta cttcattatg
    31561 agcaaactgg ctgaagcggg tatcccgact caaatggagc gtctgctctc cgataccgaa
    31621 tgtctggtga aaaagctgga tatggtgccg gttgagtgtg tcgtgcgtaa ccgtgctgct
    31681 ggctctctgg tgaaacgtct tggaatcgaa gaaggtattg agctgaaccc gccgctgttc
    31741 gatctgttcc tgaaaaacga cgccatgcac gatccgatgg tcaacgaatc ttactgcgaa
    31801 acctttggct gggtaagcaa agagaacctg gcacgcatga aagagctgac ctacaaagcg
    31861 aacgacgtgc tgaaaaaact gttcgatgat gctggtctga ttctggtcga cttcaagctg
    31921 gagtttggtc tgtacaaagg tgaagtggta ctgggcgatg agttctcccc ggacggtagc
    31981 cgcctgtggg acaaagaaac gctggagaaa atggacaaag accgtttccg ccagagcctc
    32041 ggtggcctga tcgaggccta tgaagccgtc gcccgccgcc tgggtgtaca gctggactga
    32101 tttttctgtt catcatcttg ccgtgctgct ggcacggcaa gacaaccatt ctcgtaagat
    32161 gtgcattgaa cgctcattcc tccgccattt catcccgttt tcctgtggta atcttcctct
    32221 gaaccaccta cgatcatttc tataatgaat atattgttga ggtatctatg cgttggcaag
    32281 ggcgacgtga aagtgacaat gttgaagaca ggcgcaatag ctccggcggt ccgtcaatgg
    32341 gcggtcccgg tttccgtctg ccgagcggta aaggcgggct gattttactg atagtcgtgc
    32401 tggttgcagg ctactatggc gttgatttaa ccgggttgat gaccgggcag ccggtttctc
    32461 aacagcaatc aacgcggtca attagcccaa atgaagacga agccgcaaaa ttcacctcgg
    32521 tgattctggc aaccacggaa gacacctggg gacaacagtt cgagaagatg ggtaagacct
    32581 atcagcaacc gaagctggtc atgtaccgtg gaatgacgcg taccggatgc ggggcgggcc
    32641 agtccataat ggggccgttc tattgcccgg cggatggtac ggtttatatc gatctctcct
    32701 tctatgatga catgaaagac aaacttggcg cggatggcga ttttgcccag gggtacgtta
    32761 tcgcccatga agtcggtcat catgtgcaga aactgttagg catcgagccg aaagttcgtc
    32821 aactgcaaca aaacgcgacg caggcggaag tgaaccgctt atctgtgcgt atggaactcc
    32881 aggccgactg ttttgccggt gtctgggggc atagcatgca gcagcaaggc gttctggaaa
    32941 ccggcgatct ggaagaggcg ctgaacgcgg cgcaggccat cggcgatgac cgtttacaac
    33001 agcaaagtca ggggcgagta gtaccagaca gtttcactca tggcacttct cagcaacgct
    33061 acagctggtt taaacgtggt ttcgacagcg gcgatccggc acaatgcaat acttttggta
    33121 aaagcattta acttccgggg cagggatggc tgaactgact gcgcttcaca cattaacagc
    33181 gcaaatgaaa cgtgaaggga tccgccgctt gctggtgttg agcggggaag agggttggtg
    33241 ttttgatcat gcgcttaagt tacgtgatgc cttacctggc gactggctgt ggatttcgcc
    33301 gcagccagat gctgaaaacc actgttctcc ctcggcacta caaactttac ttgggcgcga
    33361 gttccggcat gcggtattcg acgcccgcca cggctttgat gccgctgcct ttgccgcact
    33421 tagcggaacg ttgaaagcgg gaagctggct ggttttgtta ctccctgtat gggaagagtg
    33481 ggaaaaccaa cctgatgccg actcgctgcg ctggagtgat tgccctgacc ctattgcgac
    33541 gccgcatttt gtccagcatt tcaaacgcgt acttacggcg gataacgacg ctatcttctg
    33601 gcggcaaaac cagccgttct cgttggcgca ttttactccc cgtactgact ggcaccccgc
    33661 gaccggcgca ccacaaccag aacaacagca aatcttacag cagctactga ccatgccgtc
    33721 gggcgtggca gcggtaactg ctgcgcgtgg gcgcggtaaa tcggcgctgg cagggcaact
    33781 catttctcgt attgcgggta gtgcgattat cactgcgccc gcaaaagcgg caacggatgt
    33841 actggcacaa tttgcgggcg agaagtttcg ctttattgcg cctgatgcct tgttagccag
    33901 cgatgagcaa gccgactggc tggtggtcga tgaagccgca gccatacctg cgccgttgtt
    33961 gcatcaactg gtatcgcgtt ttcctcgaac gttgttaacc actacggtgc agggctacga
    34021 aggtaccgga cgtggttttt tgctgaaatt ttgcgctcgc tttccgcatt tacaccgttt
    34081 tgaactgcaa cagccgatcc gctgggcaca gggatgcccg ctggaaaaaa tggttagtga
    34141 ggcactggtt tttaacgatg aaaacttcac ccatacacca caaggcaata tcgtcatttc
    34201 cgcatttgaa caaacgttat ggcgaagcga gccagaaacg ccgttaaagg tttatcagtt
    34261 attgtctggt gcgcactacc ggacttcgcc gctggattta cgccgcatga tggatgcacc
    34321 agggcaacat tttttacagg cggctggcga aaacgagatt gccggagcgc tgtggctggt
    34381 ggatgagggg ggattatctc aagaactcag tcaggcggta tgggcaggtt ttcgtcgccc
    34441 gcggggtaat ctggtggccc agtcgctggc ggcgcacggc agcaatccac tggcggcgac
    34501 attgcgtgga cggcgggtca gccggatagc agttcatccg gcgcgtcagc gcgaaggcgt
    34561 tgggcaacag ctcattgcca gcgctttgca atataggcct ggcctcgact atctttcggt
    34621 gagttttggt tacaccgggg agttatggcg tttctggcaa cgctgcggtt ttgtgctggt
    34681 gcgaatgggt aatcatcgtg aagccagcag cggttgctat acggcgatgg cgctgttacc
    34741 gatgagtgat gcgggtaaac agctggctga acgtgagcat taccgtttac gtcgcgatgc
    34801 gcaagctctc gcgcagtgga atggcgaaac actccctgtt gatccactaa acaatgccgt
    34861 cctttctgac gacgactggc ttgaactggc cggttttgct ttcgctcatc gtccgctatt
    34921 aacatcgtta ggttgcttat tgcgtctgct acaaaccagt gaactggcat taccggcgct
    34981 gcgtgggcgt ttacagaaaa acgtcagcga cgcgcagtta tgtaccacac ttaaactttc
    35041 aggccgcaag atgttactgg tccgtcagcg ggaagaggcc gcacaggcgc tgttcgcact
    35101 taatgatgtt cgcactgagc gtctgcgcga tcgcataacg caatggcaat tttttcactg
    35161 actccttcaa ttttctccca tggtcattgt gagtcagcgg cgtaaaatta atgacatcag
    35221 ttaaggagtt cgccatgaaa catgaccatt ttgttgttca aagcccggat aaacctgcgc
    35281 aacagttgtt actgcttttc cacggtgtcg gggataaccc ggtggcaatg ggggaaatag
    35341 gtagctggtt tgcaccgttg ttcccggatg cgctggtggt gagtgtcggt ggcgcggagc
    35401 cgagcggtaa tccggcgggg cgtcagtggt tttcggtgca gggtattacg gaagataatc
    35461 gccaggcgcg tgtggatgcc atcatgccga cgtttattga aacggtgcgc tactggcaga
    35521 aacagagcgg agtaggggct aacgccacgg cgctaatcgg tttctctcaa ggcgcgatta
    35581 tggcgctgga gagcattaaa gccgaaccgg gccttgcttc acgcgtcatt gcttttaacg
    35641 gacgctatgc gagcctgccg gaaaccgcgt ctacagccac gacaattcac ttgattcatg
    35701 gcggtgaaga tccggttatc gatctggcgc atgcagttgc ggcgcaggaa gcgttaatca
    35761 gtgccggtgg tgacgtgacg ctggatatcg tggaggattt aggccatgcg atcgacaatc
    35821 gcagtatgca atttgccctg gatcacttgc gttataccat tccgaaacat tatttcgacg
    35881 aagcgctaag cggcggtaag cctggcgatg atgatgtgat tgaaatgatg taataatagc
    35941 cgggtaggcc tgataagacc gtatcaggca tcatggccgg atgcggcgtt aacgccttat
    36001 ccggccactt agcttacttc ttcggttgat cctttttcgg ccagtcgtcg tcatcgtccc
    36061 acttatcgtt gaaatcacga tgcgggggaa gctccggctt gttggcgaga aatttcttat
    36121 ggtcgacgcg cttgagatct ttaatcacgt tcagcaggac gcctaccaaa aagacaatca
    36181 ccagaatcca ccaatattta gccagccagt ccatgcttat ttcctcttgc agaaccactc
    36241 atcacgcgac gagctgttcc atgatacgtt gatacatacg ggcaagtagc tgcaggtcgg
    36301 cagcgttcac acattcatta attttatgaa tagtggcatt gaccggcccg agttccacca
    36361 cctgcgcccc catgcgggca ataaagcgcc cgtcggacgt tccgcctgtg gtcagtagct
    36421 gcggtttaat ttcattatag tgctcaaccg cgttaacgac cgcatccacc agtttaccgc
    36481 gcgcggtcaa aaatggctgc ccggaaagcc accaatccac cgtatagcgc agttgatgtt
    36541 tttcaagcag ggcaagcacc tgcgctttga tcatctcatc agtcagttcg gtgctgaagc
    36601 ggaagttaaa ctgcacaaac agttcacccg gaataacgtt gttactgccc gttcccgcct
    36661 gaatattggc aatctgcata ctggtcgccg ggaagaattc attgccctga tcccactcaa
    36721 tagccactaa ttcattaagg aaaggtgctg cgcgatgtac cggattgtca gccagatgtg
    36781 ggtaggcaac atgcccctga acgccatgaa tggttaggtt gcaggttaac gatccacgac
    36841 gaccattttt caccacatca cctaccactt cgatactcga cggttcgccg accaggcagt
    36901 aatcgagacg ctcattacgt gccattaacg cttcgacggc ttttaccgta ccattgtggg
    36961 cactggcttc ttcatcagag gtgatcagaa atgccagtcg ccctgtatgg ttgggatgtt
    37021 gtgcgacaaa acgttctgca gctaccacca tcgccgccag cgagcctttc atatctgccg
    37081 caccgcgccc gaataacatg ccgtcacgaa tggttggttc aaacggcgga ttgatccaac
    37141 gatcggcgtc gccaggcggc accacgtcgg tatgcccggc aaaggccaat gtttcacctt
    37201 gcccacgcca tgcccaaaaa ttctgcgtat cggcaaagtc catgcgttca acggtaaaac
    37261 cgatcgcctg caaacgttca atcaacaaag cctggcatcc tgcgtcatca ggactcaggg
    37321 aagggcggcg aataagctgt tgtgtcagct caataaccgg gcacgacata gactacacct
    37381 catggaaaaa tttctgataa ctggaatcac tgaaacccag cagcataggc ttaccaggcg
    37441 cgcagagcaa tggacgtttg ataattgcag gcatttcagt cattaatgcc gccgcagagg
    37501 ccgcatcggt gattatattg cgggtggttt cgtccagttt acgccaggtt gtaccacggg
    37561 tgttgagtaa cgcttcccag cctaattcgt tgataaaacc gttcaataat tcgctgtcca
    37621 gcccatcgac gcggtaatca tgaaaacgat agtcgatgtt attggcttcc agccaacggc
    37681 gagccttttt aatggtgtca caatttttga tgccgtaaag tgtaaccata atgaatcctt
    37741 tttcgattta tgagaataaa tactcattta agagtgaaaa cattttgcct gcaaatgatt
    37801 atcaggaaat ataacattat cgaagtattc aaattaacag ctattaaata atattaaaca
    37861 tttttaacgt aaacattaag aatattcatc ttgttatctg tctgtatttt aaccagaatt
    37921 aaatcagtat tagatgccgt atgaatatct cgaaatgttg ctgtgtatca catacaaatc
    37981 atcactgcag gaccatagta tttacataag gtttgtcccc cggaggtagt catcgcagca
    38041 atcgagtaaa ttagcttcac cacggtatat tgtttccgta gaatacccat aattataaga
    38101 gaggttgtta tgattgaacg tgaactgggg aactggaaag actttattga agttatgctt
    38161 cgtaagtaat tcctgagaca aagtacaaac agcaagaacc cgctgagcag gttcttaatc
    38221 ggaaagttga aaaaggcgac acattggcat gtcgcctttt ttattgctta ttccgggcgc
    38281 ggcttcagcg ggaagcggcg gcgcaccagc acaaagaaca gcggcacgaa gtaaatagcc
    38341 agaatagtgg ctgaaatcat cccgcccatt acgccagtac ccaccgcatg ctgaccaccg
    38401 gaaccggcac ccgtgctggt tgccattggc aatacgccga agataaatgc cagcgaggtc
    38461 atcagaatcg ggcgtaaacg ctgacggcag gcgtggagcg tcgcttcaaa caggtcgtgg
    38521 cctttttggt tcatctcgtt agcaaactcg acgatcagga tggcgttttt cgccgataaa
    38581 ccaatgaccg ttaacaggcc cacctggaag taaacgtcgt tttccagccc gcgcatccag
    38641 gttgccagca gcgcgccgat tacccccagc ggcacgacca gcattaccga gaacggtacc
    38701 gaccagcttt catacaatgc agccagacac aggaatacca ccagcaagga aatggcgtac
    38761 agcgccggag cctgcgcgcc ggaaagccgc tcctgatacg acatcgccgt ccactccaga
    38821 ccaaagccgt ttggtagctg cttcactaac gattccataa tatccatcgc cgtaccggta
    38881 ctgacccccg gtgcggcttc cccaacaatt tcaaccgcag aataaccgtt atagcgttcc
    38941 agacgcggcg agcctgtctc ccagcgtgag gtcgcgaaag cagagaaggg caccatgccg
    39001 ccatctttat ttcggacata ccagagattg atgtcatctg gcagcatgcg atacggcgca
    39061 gctgcctgca catagacttt cttcacgcga ccgcgatcca taaagtcatt cacatagctc
    39121 gaaccccagg cggtttgcag tgtgtcgtta atatcgtcga tagcaacgcc cagcgcctga
    39181 gctttacgct ggtcgatatc aatctgcaac tgcggactgt catcgaggcc gttatggcgc
    39241 acacgggtta gctccgggtt ttccgccgcc agcgccagca actggttacg tgctgccatc
    39301 agcgcatcgt gacctgctcc agcgtggtcc tgcaactcca tatcaaaacc tgcagaacta
    39361 ccaagaccgc taattgctgg cgggctgctg gcgataacgc gagcttcttt aatttggtta
    39421 aacgcctttg ttgcacgctc gataatggca aacgaggtgc cggtcttact gtcgcgttcg
    39481 ctccagtctt tcaggcggat aaacattcgc gccacgtttt gcccgttacc cccagggcca
    39541 gaaccaacag tggcaaacac cgacatgatg ttgtcttttt catgggtgaa gtaatatttc
    39601 tcgatttgct caacgacttt cagggtctgt tgttgggttg aaccgctggg caattgtacc
    39661 gaggtagtaa acatgccacg gtcttccagc ggtaagaacg acgtcggcaa acgcaggaac
    39721 aggaacacca tgccgccaag cagcaggaca taaatcacaa tccagcgcag gctacggtgg
    39781 agaattttcg ccaccccttt ttcgtagcgt tcggcgttgc ggttaaacat ctggttaaac
    39841 caggcaaaaa agcctttttg cccgtgatgc tcaccttttt tcagtggctt aagtagtgtg
    39901 gcacacagag ccggagtgag gatcatcgct accagtactg acagcaccat cgccgcaaca
    39961 atggtaatag agaactggcg atagatggca ccggtggtgc cgccgaagaa ggccattggt
    40021 acaaataccg ccgaaagcac catcgcaatc ccgaccagtg ccccctggat ctgccccatc
    40081 gatttacgcg tggcttcgcg aggagtgagt ccttcctcac tcataatacg ttcgacgttt
    40141 tccaccacca cgatggcgtc atccaccagc agaccgatcg ccagcaccat cgcgaacatg
    40201 gttaaggtgt tgacgctgta accgaaggcg taaagtacgg agaaggttcc catcaacacc
    40261 accggcacgg cgatagtggg tatcagcgtg gcgcggaagt tttgcaggaa cagatacata
    40321 acgaggaaaa ccagagcgat agcttccagc agcgttttca ccacgtcttc aatggaggct
    40381 ttaacaaacg aggtggtttc atacgccacc ttgtattcca gtccgtgcgg gaaatactgc
    40441 gccagctcgt cgagacgatt gagcaccagc tccgctgtcg ccatttcgtt cgcgccggag
    40501 gccagtttta cccccaaccc ggaggctggc ttaccattga agcggctaag ataatcgtat
    40561 ttctccgccc ccatttcggc ggtggcgaca tcgccgagcc ttacttctga gccgtcctga
    40621 ttcacccgca aggtgatatc gcggaactgt tctggtgttt gcagcagtga ctgggcgtta
    40681 atggtggcgt tgagcgcctg cttatcgacg gaaggtgtac caccaagttg cccaaccgca
    40741 atctgcgcgt tctgtgactc aatggcatca gtgacatctt tagctgtcat ctggaaactg
    40801 ttgagtttcg ccgggtccag ccagatacgc atggaatatt gcgaaccata ggcatcgata
    40861 tccccgacgc catttacgcg gcttaacggg tcctgaatat tactggcaac ataatcagca
    40921 atatcctgtt tatccatcga accatcggta gagacgaagg caatggtcag aatgttggta
    40981 tcgccggttt tacgcacagt cacgccctga ttttgcaccg cctgcggtaa ctttcgcatg
    41041 gctgattgca gctggttttg tacttgctgc acggcttcgt ccggatcggt gcctgcttta
    41101 aaacttaaag tgacagatgc ctgaccggtg ccactgctct gagatgacat atacatgaga
    41161 ttatcgaggc cggtcatatt ttgctcgata acctgggtca cggtgttttc cagcgtctgg
    41221 gccgatgcgc cgggatagtt agcggtcact cgcacattcg gtggcgcgag atcggggtat
    41281 tgttcaacgg gcaatgaaaa aatcgccagg gtacctgtca gacacaacag gattgccagc
    41341 acccaggcaa aaatggggcg atcaataaag aaattcgcca ttaaaagagg acctcgtgtt
    41401 tctgcgtatc gttgtaggct tgacttgctt cactgtagcg gcaaggtacg agccaatcgt
    41461 ggagaaaaaa aggagttaat gtaaattatc atgccctttt atactgaacc ttaactgcaa
    41521 gtattctttg gtcagtagga ggcattacat caactgactt tcctcaccct ccgcagagcg
    41581 aaagctaata ctcactaacg tgccgccgcc ggaaggttgt gaaaaagtca gcgtcccacc
    41641 tagccgttcc gcgcgttcgc gcatgatatt cagaccataa tgaccttcgg gttctttcgg
    41701 ttcaccgata ccaatcccgt tatcacggat ataaaccgtg tgattgccgt ccggcgcggt
    41761 gacgcaactg acggcgattt cgctggcgtt ggcgtgcttc atcgcattca gcaccgcttc
    41821 gcgaataatt tgcaacaaat gcacctgcat ttgcgcatcc agtgccaggg ttggcagacg
    41881 gcagtcgagg gtcagtttgg cgctggtttg attttgtaac gtatccagca tttccctcaa
    41941 cgcggagggg agatccgcct gctgcagcgt caggcgaaaa gtagtcaaca gctcgcgtaa
    42001 ctgccgataa gcatcattca acgcctggga aaaatcggcc atgatacttt gtgcggtggc
    42061 gttatcttcc ggtatcgaac gcttaagtaa cgtcaactgg atacgtaagt aagaaagtac
    42121 ctgagccagc gagtcgtgca attcgcgggc gatggttgca cgttcttcca tcaacaataa
    42181 ctgctgaaaa tgcttctgcg cctgattaaa gtacaaaccg cgtcccagca tcgacgaaac
    42241 gctgttaagc agcggttctg atgatgaaac gtgactattt tgccagtgca gttcgccgta
    42301 aaccgtctct tgcattgtca ccggtaaaat ctgcatcggc aattccgggt ttggttgccc
    42361 ttcgctaatc cgccagtttt caccgacatt taactccaga tattcagccg cttcattgtc
    42421 gcgaacaatc tgcaaaatat ggcggaaaca atgcacatca atctggctgg tgttcagcgc
    42481 ctgcgaacat tgatacaaca cctccagacg acgcttggcc tcgtggagat cgcgggtctt
    42541 ttcttctact gacgcctcca gcgaacggta caatttatgc agctcgctcg acatctggtt
    42601 aaaggttttt gcaagtagac caagctcatt cggcaggctg gtatccagcg gtggcgagtc
    42661 gaactgtccg tgttcaatac gctgactggc ggtaaccagc tgattcagcg gggcaaccac
    42721 ctgatggcgt atgcggccca aagtaaaaaa gaccagcgtg aaaataccga taccgccagc
    42781 cagggaaatc gccaccacca gcagcatttt gcgttcagcg tagtgctgta aagccagcac
    42841 gaacaggtct atctgattaa cataattatt aatattggcc tgataccacg gcaaatcgcc
    42901 cttgctgagc cgattattca tctccagcca gttggcattc agatgcgcat agcgagtttt
    42961 tactgcttct ggcacatacc agacgttgag gttggttaat accggtgaat gcagtgcctg
    43021 ctgaaacagc tggcgatgtg cattgagttg tggagtgcca ctttgcaagt cgtagcccag
    43081 gcggtaactc tgcatacgca gcgatccggc aatattgata gcctcagcgt cgcgcaaact
    43141 gctcgccaga gttagcagag cgatacccgt ggaaagaatc gacagcagca caatgtaaaa
    43201 aaaggcccgg gccagactgg ccgagacggg tcgtttaaca atcacacgcg tcttctccac
    43261 aaaaaatcat taatgttcca gttcgctgaa acgcaattta gttagtgatg ggattatttg
    43321 atctgccaca gtttatagcg aaatagttac ttattctggg cacatgaaca ttgccgattc
    43381 ccctgtaagt cgggtaataa caacactcat ataaagaata aggtttttac aaccaaaaaa
    43441 gaaggtcgtt atgaatcgtt ttattatggc caacagtcag caatgtctgg gttgtcatgc
    43501 ttgtgaaatc gcttgtgtca tggctcacaa tgatgagcaa catgtcctga gccaacacca
    43561 ttttcatccc cgaattacgg ttatcaaaca tcagcagcaa cgtagtgcag tgacctgtca
    43621 ccattgtgaa gatgcgccct gcgcccgtag ctgccctaat ggcgcaatca gccacgttga
    43681 tgacagcatt caggtcaatc agcaaaagtg tattggctgt aaatcctgcg tggtggcctg
    43741 tccgtttggt acgatgcaaa tcgtcctgac acccgtcgca gcgggcaaag ttaaagccac
    43801 ggcgcataaa tgcgaccttt gtgcagggcg cgaaaacggt cctgcctgtg tcgagaattg
    43861 cccggcggac gcgctgcagc tggtcactga cgccgcactc tcaggcatgg cgaaatcccg
    43921 ccgcttgcgt accgcacgtc aggaacatca accgtggcat gccagtaccg ctgcacaaga
    43981 aatgccggta atgagtaaag tcgaacaaat gcaggcaacg cccgcgcgtg gcgagccgga
    44041 taaactggcg attgaagcgc gcaaaaccgg ttttgatgaa atttatctgc cattccgtgc
    44101 cgatcaggca caacgggaag cctcgcgctg ccttaaatgc ggcgagcaca gcgtttgtga
    44161 atggacctgc ccgctgcata accatatacc gcagtggatt gaactggtga aagccggaaa
    44221 catcgacgcc gccgtcgagc tttctcacct gaccaacacc ctgccggaaa ttaccggacg
    44281 cgtttgtccg caagaccgtt tgtgtgaagg tgcctgtact attcgcgatg agcacggtgc
    44341 ggtaactatc ggcaacattg aacgctacat ttcagatcag gcgttggcga aaggttggcg
    44401 tcctgactta agccatgtca ccaaagtgga caagcgggtg gcgattatcg gtgcaggtcc
    44461 ggcagggctg gcctgtgcgg atgttctgat ccgcaatggc gtggcggtga cggtgtacga
    44521 tcgccatcca gaaatcggtg gcttgctcac tttcggcatt ccttctttca aactggataa
    44581 atccctgctg gcacgtcgtc gggaaatctt cagcgcgatg gggattcact tcgaactcaa
    44641 ttgtgaagtg ggtaaagatg tctctttgga ttcgcttttg gaacaatacg acgcggtctt
    44701 cgttggcgta ggcacttacc gttccatgaa agcgggttta cccaatgaag atgcgccggg
    44761 cgtttatgac gcgctgccgt tcctcatcgc caacactaaa caggtgatgg ggctcgaaga
    44821 gctaccggaa gagccgttta tcaataccgc cggacttaac gtcgtggtac tgggcggcgg
    44881 cgacaccgcg atggactgtg tgcgtactgc gctgcgccac ggcgcgagta acgtcacctg
    44941 cgcttatcgt cgtgatgaag ctaacatgcc aggctcgaag aaagaggtga agaacgcccg
    45001 cgaagagggg gccaacttcg aatttaacgt ccagccggtg gcgcttgagc tgaatgaaca
    45061 aggtcacgtc tgcgggattc gtttcctgcg cacgcgtctt ggcgagccgg atgcccaggg
    45121 gcgtcggcgt ccagtgccgg tggaaggcag tgaatttgtc atgccagccg acgcggtgat
    45181 tatggcgttt ggcttcaatc cgcacgggat gccgtggctg gagtcgcacg gtgtaacggt
    45241 agacaaatgg ggccgcatca tcgcggatgt ggaaagccag taccgttacc agaccaccaa
    45301 tccgaaaatc ttcgccggtg gcgacgccgt gcgtggtgcg gatctggtgg ttaccgcaat
    45361 ggcagaagga cgtcatgcgg cacaggggat tattgactgg ctgggggtaa aatcagtcaa
    45421 atctcactga tagcctgcgc agacaaaccc gacttcacag cgtaatataa ttgttcattt
    45481 cgcgctgtgg agtcggtatg acgcaacaaa tcaccctcat taaagacaaa attctctccg
    45541 ataactattt caccctgcac aacattactt acgatctcac gcgcaaagat ggcgaagtta
    45601 tccgccataa acgtgaagtt tacgatcgcg gtaatggtgc gacgatcctc ctgtacaacg
    45661 cgaagaaaaa gaccgtggtt ctgattcgtc agttccgtgt cgctacctgg gttaatggca
    45721 atgaaagcgg gcagctgatt gaaacctgcg ccgggctgct ggataacgac gaaccggaag
    45781 tgtgcattcg caaagaagcg attgaagaga cgggctatga agtgggcgaa gtgcgcaaat
    45841 tatttgaact gtatatgtcg ccaggcggtg tgactgagct aatccacttt tttatcgccg
    45901 aatacagtga caatcagcgc gctaacgccg gtggcggtgt cgaagatgaa gatattgaag
    45961 tgctcgagct gccgttcagc caggcgctgg agatgatcaa aaccggcgag atacgtgacg
    46021 gtaagacggt gttattgctt aactatttgc aaatgtcaca tttaatagac tgaaaaataa
    46081 caataatatt tcgttgttta ttcttggctc aatccgacaa atccgattga gccgcgctac
    46141 ctgcgcaacg aagatacgac ttgtgctgtt tgtttgaact tctggggtcg taccgtccat
    46201 gcgctatcgc attttccttc tctttttttt cgctttgttg ccgacgtctt tggtgtgggc
    46261 ggcaccagcg caacgggcgt tttccgactg gcaggtcacc tgtaataacc aaaacttctg
    46321 cgtggcgcgt aatacgggcg atcataatgg actggtgatg accctgagcc gcagcgccgg
    46381 ggcgcatacc gatgccgttt tacgtattga gcgcggcggg ttgaagtcgc cggatgcgtc
    46441 tgaaggggaa attgcgccac ggctgctgtt agatggcgag ccgttggcgt taagtggtga
    46501 caagtggcgg atttcaccat ggttattagt aaccgatgat acggcaacca tcaccgcgtt
    46561 tttgcagatg attcaggaag ggaaggcgat caccttacgc gacggcaatc agaccatttc
    46621 tctgagtgac ttaaaagcgg cgttgttgtt tattgatgct cagcaaaagc gcgttggcag
    46681 tgaaaccgcg tggatcaaga aaggggacga accgccgctc agcgtaccgc ccgcgcctgc
    46741 gctgaaagag gtcgtggtgg ttaacccaac gccgacgcca ctctcactcg aagaacgcaa
    46801 cgatttgctg gattatggaa actggcggat gaatggtctg cgctgctcgc ttgatccatt
    46861 gcgtcgtgag gtgaatgtca ctgcgctgac tgatgataaa gcgctgatga tgattagctg
    46921 tgaggcaggg gcctataaca ccattgattt ggcatggatt gtgtcgcgta aaaagccgct
    46981 ggcctcgcgc ccggtccggc tgcgtttgcc gttcaacaac ggtcaggaga cgaatgaact
    47041 ggaactgatg aacgcaacat ttgatgagaa atcgcgtgaa ctggtgacct tagcgaaagg
    47101 gcgcggatta agcgattgtg gcattcaggc gcgctggcgc tttgacggtc agcgttttcg
    47161 cctggtgcgt tacgccgcag aacccacctg cgataactgg catgggccag acgcctggcc
    47221 cacgttgtgg atcacccgtt aaacaaaatg gcgggacatg taggccggat aagacgctgt
    47281 aggccggata agacgcgtca gcgtcgcatc cggcaatcag catccggcga tcaccatcag
    47341 gcacctttta ctcccagcac cttatgcgct tttgccacaa tattctcggc ggtaaagcca
    47401 aagaacggga acagcttatc cgccggagca gattccccat aacccgtcat cccgacaatt
    47461 gcccctttca gaccaacata cttgtaccag taatcggcaa tacctgcttc caccgccacg
    47521 cgagccgcaa cgttagaagg caacaccgac tcccgatatt cctcatcctg ggcgtcgaaa
    47581 atatcggtcg agggcaggga aaccacgcga acattgcgac cttctcccgc taatttctcc
    47641 gccgcttgca gggtgatttc catctctgaa ccggtggcaa tcaaaataat atctggctta
    47701 ccgccgctgt ctttcagcac ataaccgcca cgagcaatct ctttaacctg atccggcgta
    47761 cgttccacct gggccagatt ctgcctggag agaatcagtg ccgtcggtcc gttgtggcgc
    47821 tcaaccgcca gcttccagcc caccgccgct tccacctgat cgcacggtcg ccaggtgctg
    47881 aaatttggcg ttaagcgcag gctggccagt tgctcaacag cctggtgcgt cggaccatct
    47941 tcgcccagac caattgagtc gtgggtataa accataatct gccgcgcttt catcagtgcc
    48001 gccatccgcg cggcgttacg ggcgtattca acaaacatca ggaacgttgc ggtatacggc
    48061 acaaagccgc cgtggtgcgc aatgccgttg gcgatagcgg tcatgccaaa ttcacgcacc
    48121 ccgtagtgaa tgtagttgcc cgccggatct tccttcagcg aaacagaacc tttccagatg
    48181 gtcaggttgc tgggagccag atccgccgaa ccgccgagca gctccggtag catcggcccg
    48241 taagcgttaa gcgtattttg cgaagcctta cgggtagcga ttttcgccgg attcgcctgc
    48301 aactcattga tatatttctg agtcgttttc tcccagtcct tcggtaaacc accgctcatc
    48361 cgacgggtaa actcttctgc cagttgcgga tgagcctttt tataggcggc aaacttctca
    48421 ttccagctct gctgcgcttt ttcgcctttt tcgcgggcat cccaagcgtg ataaatatct
    48481 ttagggatct caaatggcgg atggtgccag cccagttttt gccgtaccag cgccacttct
    48541 tcttccccca gcggtgcgcc gtgcgcctct tccttacctg ctttattcgg cgaaccaaag
    48601 ccaatcaccg tacggcagat aatcagcgac ggcttatctt tcacgctttg cgcttcaagg
    48661 attgcttcct tcaccgcctg cggatcgtga ccgtcgattt catggatcac atgccagtga
    48721 taggcttcaa aacgttttgc cgtatcgtcg gtaaaccagc cttcggtttc tccatcaatc
    48781 gaaataccgt tgtgatcgta aaaaccaatc agcttgccca gtcccagcgt gcccgccagc
    48841 gaacagactt cgtgggaaat accttccatc aggcagccgt cgcccataaa cacataggtg
    48901 aagtgatcga caatttcatg atccggctgg ttaaactgcg ccgccagtgt acgttccgct
    48961 atcgccagcc cgacggcgtt cgccaaacct tgtccaagag gaccggtggt ggtttcaact
    49021 ccgggcgtat agccgatttc cgggtggcca ggggttttcg aatgcagttg acgaaagttc
    49081 ttcagctctt ccagcggcag gtcgtaaccg gtcagatgca gcaaactgta gagcagcatc
    49141 gacgcgtgac cgttggaaag aataaagcgg tcgcgatcat accaggttgg gtcggtaggg
    49201 ttatgtttaa gaaaatcgtt ccacagcact tcggcaatat cagccatgcc catcggcgcg
    49261 ccgggatgac cagaattggc tttttgtacc gcatccatac tgagtgcgcg aatcgcattg
    49321 gcaaggtctt ttcgggacat ataacactcc gtggctggtt tatagtttgg cggcaagaag
    49381 atcttccagt ttgcgttgat caacggcgaa cagacgaatg ccttcagaca gtttttctac
    49441 cgccatcgca tcctgattgt gctcccaacg gaactccgct tcgctcatgg gagctgggcg
    49501 tgggaacgtc tgagaaggtg ggattaattt acgtaccact ggcgaaactt tttcctgcag
    49561 ctccttcagc aaattcggtg cgatagtcag tcgatcgcag ccggttaagg cgaggatttg
    49621 ttcggtgcga cggaagctcg cgcccatcac aatggtttca tagtggtgtt gcttatagta
    49681 gtcgtagata ttgcgcaccg atttaacgcc cggatcttct tccaccacat atgggtccat
    49741 cggcttgcgt gcctgatacc agtcataaat acgcccgaca aacggcgaaa tcagaaaaac
    49801 gcccgcttct gcacaggccc gcgcctgtgc aaaagaaaac agcagcgtca ggttgcagtt
    49861 aataccttct ttttccagct cttctgccgc gcgaattcct tcccaggtcg aagccagttt
    49921 gatcagaatg cgtgatttct caacgccttg ttgctgatac aagtccacca gatggcgcgc
    49981 cttctcaata ctcttttctt tatcaaaaga gaggcgtgca tcaacttctg ttgacacgcg
    50041 accgggtacg attttgagga tttcagcacc gaaattgacc gccagtttgt cacacgctgc
    50101 gaccacctgt tgttcctggg tcttgccatt ttttttaccc caggcgatag catcgtctat
    50161 taaatgctca tattgtgata atccggcagc cttgagtaac agcgaaggat tggtggtggc
    50221 atcctgggga tgataatggc gaatggactc aatatcgccg ctgtctgcca cgacagtggt
    50281 gaactgtttg atgccgtcta actcgttcat aggaaatact ccttgaaaag taaagtgtta
    50341 gatgagtgcg ttaattcaca cttctgagaa atttcgctaa acgcatcaaa aaagcatagc
    50401 agacaggcat ggtattgctg gattaagcag gtaacatcag tgttatagga ttattaccaa
    50461 aacattatat taatggtgag agtttggact tgctcaaagt ctgtagactc cggcaaggta
    50521 ataatgtgcg ccacgttgtg ggcaggggct gttgcccaca cactttattt gtgaacgtta
    50581 cgtgaaagga acaaccaaat ggatgaccag ttaaaacaaa gtgcacttga tttccatgaa
    50641 tttccagttc cagggaaaat ccaggtttct ccaaccaagc ctctggcaac gcagcgcgat
    50701 ctggcgctgg cctactcacc aggcgttgcc gcaccttgtc ttgaaatcga aaaagacccg
    50761 ttaaaagcct acaaatatac cgcgcgcggt aacctggtgg cggtgatctc taacggtacg
    50821 gcggtgctgg ggttaggcaa cattggcgcg ctggcaggca aaccggtgat ggaaggcaag
    50881 ggcgttctgt ttaagaaatt cgctgggatt gatgtatttg acattgaagt tgatgaactc
    50941 gacccggaca aatttattga agtcgtcgct gcgctcgaac cgaccttcgg cggcatcaac
    51001 ctcgaagaca ttaaagcgcc agaatgtttc tatattgaac agaaactgcg cgagcggatg
    51061 aatattccgg tattccacga cgatcagcac ggcacggcaa ttatcagcac tgccgccatc
    51121 ctcaacggct tgcgcgtggt ggagaaaaac atctctgacg tgcgtatggt ggtttccggc
    51181 gcgggtgctg cagcaatcgc ctgtatgaac ctgctggtag cgttgggtct gcaaaaacat
    51241 aacatcgtgg tttgcgattc aaaaggcgtt atctatcagg gccgtgagcc aaacatggcg
    51301 gaaaccaaag ccgcatatgc ggtagtggat gacggcaaac gtaccctcga tgatgtgatt
    51361 gaaggcgcag atattttcct cggctgttcc ggcccgaaag tgctgaccca ggaaatggtg
    51421 aagaaaatgg ctcgagcgcc aatgatcctg gcgctggcga acccggaacc ggaaattctg
    51481 ccgccgctgg cgaaagaagt gcgtccggat gccatcatct gtaccggccg ttccgactac
    51541 ccgaaccagg tgaacaacgt cctttgcttc ccgttcatct tccgtggcgc gctggacgtt
    51601 ggcgcaaccg ccatcaacga agagatgaaa ctggcggcgg tacgcgcgat tgcagaactg
    51661 gcccatgcgg aacagagtga agtggtggct tcagcgtatg gcgatcagga tctgagcttt
    51721 ggccctgaat acatcattcc aaaaccgttt gatccgcgtc tgatagtcaa gattgctcct
    51781 gcggtcgcga aagcggcgat ggagtcgggt gtggcgacgc gtccgattgc tgatttcgac
    51841 gtctacatcg acaagctgac tgagttcgtt tacaaaacca acctgtttat gaagccgatt
    51901 ttctcccagg ctcgcaaagc gccgaagcgc gtagttctgc cggaagggga agaggcgcgc
    51961 gttctgcatg ccactcagga actggtaacg ctgggactgg cgaaaccgat ccttatcggt
    52021 cgtccgaacg tgatcgaaat gcgcattcag aaactgggct tgcagatcaa agcgggcgtt
    52081 gattttgaga tcgtcaataa cgaatccgat ccgcgcttta aagagtactg gaccgaatac
    52141 ttccagatca tgaagcgtcg cggcgtcact caggaacagg cgcagcgggc gctgatcagt
    52201 aacccgacag tgatcggcgc gatcatggtt cagcgtggcg aagccgatgc aatgatttgc
    52261 ggtacggtgg gtgattatca tgaacatttt agcgtggtga aaaatgtctt tggttatcgc
    52321 gatggcgttc acaccgcagg tgcaatgaac gcgctgctgc tgccgagtgg taacaccttt
    52381 attgccgata cctacgtcaa tgacgaacca gacgcagaag agctggcgga gatcaccttg
    52441 atggcggcag aaactgtccg tcgtttcggt attgaaccgc gcgttgctct gttgtcgcac
    52501 tccaactttg gttcttctga ctgcccgtcg tcgagcaaaa tgcgtcaggc gctggaactg
    52561 gtcagggaac gtgcgccaga actgatgatt gatggtgaaa tgcacggcga tgcagcgctg
    52621 gtggaagcga ttcgcaacga ccgtatgccg gacagccctt tgaaaggttc tgccaatatt
    52681 ctggtgatgc cgaacatgga agctgcccgc attagttaca acttactgcg cgtttccagc
    52741 tcggaaggtg tgactgtcgg tccggtgctg atgggcgtgg cgaaaccggt tcacgtgtta
    52801 acgccgatcg catcggtgcg tcgtatcgtc aacatggtgg cgctggccgt ggttgaagcg
    52861 caaacccaac cgctgtaatt cttttttaac tctcacgctt accctgaata ttcagggtaa
    52921 gcggtttagc tgcaatatat tagtaaagct tattactgag tttgc
//
LOCUS       DBFIFM010000009        46520 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.009, whole genome shotgun sequence.
ACCESSION   DBFIFM010000009 DBFIFM010000000
VERSION     DBFIFM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.009"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(156..698)
                     /gene="ycfP"
                     /locus_tag="N7C74_000486"
     CDS             complement(156..698)
                     /gene="ycfP"
                     /locus_tag="N7C74_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HGU2430426.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(721..1746)
                     /gene="nagZ"
                     /locus_tag="N7C74_000487"
     CDS             complement(721..1746)
                     /gene="nagZ"
                     /locus_tag="N7C74_000487"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HGU2430427.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQVSLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(1757..2581)
                     /gene="thiK"
                     /locus_tag="N7C74_000488"
     CDS             complement(1757..2581)
                     /gene="thiK"
                     /locus_tag="N7C74_000488"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HGU2430428.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLHQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2562..3203)
                     /gene="lpoB"
                     /locus_tag="N7C74_000489"
     CDS             complement(2562..3203)
                     /gene="lpoB"
                     /locus_tag="N7C74_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HGU2430429.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3217..3594)
                     /gene="ycfL"
                     /locus_tag="N7C74_000490"
     CDS             complement(3217..3594)
                     /gene="ycfL"
                     /locus_tag="N7C74_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HGU2430430.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3597..3956)
                     /gene="hinT"
                     /locus_tag="N7C74_000491"
     CDS             complement(3597..3956)
                     /gene="hinT"
                     /locus_tag="N7C74_000491"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HGU2430431.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4087..4275
                     /locus_tag="N7C74_000492"
     CDS             4087..4275
                     /locus_tag="N7C74_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012138106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2430432.1"
                     /translation="MLPYISPLFPFLPANALKRHSLFGLHDKYECVYFSFAFAFAFAF
                     ASKIIRTLNITAHHPPPA"
     gene            4308..6497
                     /gene="fhuE"
                     /locus_tag="N7C74_000493"
     CDS             4308..6497
                     /gene="fhuE"
                     /locus_tag="N7C74_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HGU2430433.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(6557..7990)
                     /gene="ptsG"
                     /locus_tag="N7C74_000494"
     CDS             complement(6557..7990)
                     /gene="ptsG"
                     /locus_tag="N7C74_000494"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HGU2430434.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8285..9082)
                     /gene="ycfH"
                     /locus_tag="N7C74_000495"
     CDS             complement(8285..9082)
                     /gene="ycfH"
                     /locus_tag="N7C74_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU2430435.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9093..10097)
                     /gene="holB"
                     /locus_tag="N7C74_000496"
     CDS             complement(9093..10097)
                     /gene="holB"
                     /locus_tag="N7C74_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HGU2430436.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10094..10735)
                     /gene="tmk"
                     /locus_tag="N7C74_000497"
     CDS             complement(10094..10735)
                     /gene="tmk"
                     /locus_tag="N7C74_000497"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGU2430437.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEIITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTRWVKELDA"
     gene            complement(10725..11747)
                     /gene="yceG"
                     /locus_tag="N7C74_000498"
     CDS             complement(10725..11747)
                     /gene="yceG"
                     /locus_tag="N7C74_000498"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HGU2430438.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRYLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYITVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAIASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11750..12559)
                     /gene="pabC"
                     /locus_tag="N7C74_000499"
     CDS             complement(11750..12559)
                     /gene="pabC"
                     /locus_tag="N7C74_000499"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HGU2430439.1"
                     /translation="MFLINGYKQESLSVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGITLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGGVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12679..13920)
                     /gene="fabF"
                     /locus_tag="N7C74_000500"
     CDS             complement(12679..13920)
                     /gene="fabF"
                     /locus_tag="N7C74_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HGU2430440.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14008..14244)
                     /gene="acpP"
                     /locus_tag="N7C74_000501"
     CDS             complement(14008..14244)
                     /gene="acpP"
                     /locus_tag="N7C74_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGU2430441.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14455..15189)
                     /gene="fabG"
                     /locus_tag="N7C74_000502"
     CDS             complement(14455..15189)
                     /gene="fabG"
                     /locus_tag="N7C74_000502"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HGU2430442.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILVQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15202..16131)
                     /gene="fabD"
                     /locus_tag="N7C74_000503"
     CDS             complement(15202..16131)
                     /gene="fabD"
                     /locus_tag="N7C74_000503"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HGU2430443.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQHGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIGKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16147..17100)
                     /gene="fabH"
                     /locus_tag="N7C74_000504"
     CDS             complement(16147..17100)
                     /gene="fabH"
                     /locus_tag="N7C74_000504"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HGU2430444.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17168..18238)
                     /gene="plsX"
                     /locus_tag="N7C74_000505"
     CDS             complement(17168..18238)
                     /gene="plsX"
                     /locus_tag="N7C74_000505"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HGU2430445.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18319..18492)
                     /gene="rpmF"
                     /locus_tag="N7C74_000506"
     CDS             complement(18319..18492)
                     /gene="rpmF"
                     /locus_tag="N7C74_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HGU2430446.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18544..19065)
                     /gene="yceD"
                     /locus_tag="N7C74_000507"
     CDS             complement(18544..19065)
                     /gene="yceD"
                     /locus_tag="N7C74_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HGU2430447.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            19264..19839
                     /gene="yceF"
                     /locus_tag="N7C74_000508"
     CDS             19264..19839
                     /gene="yceF"
                     /locus_tag="N7C74_000508"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HGU2430448.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPHSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNR"
     gene            complement(19948..20907)
                     /gene="rluC"
                     /locus_tag="N7C74_000509"
     CDS             complement(19948..20907)
                     /gene="rluC"
                     /locus_tag="N7C74_000509"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HGU2430449.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAR"
     gene            21480..24665
                     /gene="rne"
                     /locus_tag="N7C74_000510"
     CDS             21480..24665
                     /gene="rne"
                     /locus_tag="N7C74_000510"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HGU2430450.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGGEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETREGRQQAEVTEKARTADEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEETV
                     VAPVAEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVTAVKPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(24860..25813)
                     /gene="flgL"
                     /locus_tag="N7C74_000511"
     CDS             complement(24860..25813)
                     /gene="flgL"
                     /locus_tag="N7C74_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HGU2430451.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDNDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEVNGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(25825..27468)
                     /gene="flgK"
                     /locus_tag="N7C74_000512"
     CDS             complement(25825..27468)
                     /gene="flgK"
                     /locus_tag="N7C74_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HGU2430452.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDEGEDFFAIGKPA
                     VLQNTKNNGNVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(27534..28475)
                     /gene="flgJ"
                     /locus_tag="N7C74_000513"
     CDS             complement(27534..28475)
                     /gene="flgJ"
                     /locus_tag="N7C74_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HGU2430453.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTTGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(28475..29572)
                     /gene="flgI"
                     /locus_tag="N7C74_000514"
     CDS             complement(28475..29572)
                     /gene="flgI"
                     /locus_tag="N7C74_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HGU2430454.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAIA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(29584..30282)
                     /gene="flgH"
                     /locus_tag="N7C74_000515"
     CDS             complement(29584..30282)
                     /gene="flgH"
                     /locus_tag="N7C74_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HGU2430455.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(30335..31117)
                     /gene="flgG"
                     /locus_tag="N7C74_000516"
     CDS             complement(30335..31117)
                     /gene="flgG"
                     /locus_tag="N7C74_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HGU2430456.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(31255..32010)
                     /gene="flgF"
                     /locus_tag="N7C74_000517"
     CDS             complement(31255..32010)
                     /gene="flgF"
                     /locus_tag="N7C74_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HGU2430457.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADITPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(32030..33235)
                     /gene="flgE"
                     /locus_tag="N7C74_000518"
     CDS             complement(32030..33235)
                     /gene="flgE"
                     /locus_tag="N7C74_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HGU2430458.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGAEPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(33260..33955)
                     /gene="flgD"
                     /locus_tag="N7C74_000519"
     CDS             complement(33260..33955)
                     /gene="flgD"
                     /locus_tag="N7C74_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HGU2430459.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(33967..34371)
                     /gene="flgC"
                     /locus_tag="N7C74_000520"
     CDS             complement(33967..34371)
                     /gene="flgC"
                     /locus_tag="N7C74_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HGU2430460.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(34375..34791)
                     /gene="flgB"
                     /locus_tag="N7C74_000521"
     CDS             complement(34375..34791)
                     /gene="flgB"
                     /locus_tag="N7C74_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HGU2430461.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRNATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            34946..35605
                     /gene="flgA"
                     /locus_tag="N7C74_000522"
     CDS             34946..35605
                     /gene="flgA"
                     /locus_tag="N7C74_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HGU2430462.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSSQLAGISDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLGAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            35681..35974
                     /gene="flgM"
                     /locus_tag="N7C74_000523"
     CDS             35681..35974
                     /gene="flgM"
                     /locus_tag="N7C74_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HGU2430463.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            35979..36395
                     /gene="flgN"
                     /locus_tag="N7C74_000524"
     CDS             35979..36395
                     /gene="flgN"
                     /locus_tag="N7C74_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HGU2430464.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEIAVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(36435..37970)
                     /gene="murJ"
                     /locus_tag="N7C74_000525"
     CDS             complement(36435..37970)
                     /gene="murJ"
                     /locus_tag="N7C74_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HGU2430465.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(38080..39003)
                     /gene="yceM"
                     /locus_tag="N7C74_000526"
     CDS             complement(38080..39003)
                     /gene="yceM"
                     /locus_tag="N7C74_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU2430466.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYCELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITEMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(39005..39652)
                     /gene="yceH"
                     /locus_tag="N7C74_000527"
     CDS             complement(39005..39652)
                     /gene="yceH"
                     /locus_tag="N7C74_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HGU2430467.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVELTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(39663..40247)
                     /gene="rimJ"
                     /locus_tag="N7C74_000528"
     CDS             complement(39663..40247)
                     /gene="rimJ"
                     /locus_tag="N7C74_000528"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HGU2430468.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            40483..41691
                     /gene="mdtH"
                     /locus_tag="N7C74_000529"
     CDS             40483..41691
                     /gene="mdtH"
                     /locus_tag="N7C74_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HGU2430469.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSVHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            41755..42402
                     /gene="grxB"
                     /locus_tag="N7C74_000530"
     CDS             41755..42402
                     /gene="grxB"
                     /locus_tag="N7C74_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HGU2430470.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            42536..43096
                     /gene="yceB"
                     /locus_tag="N7C74_000531"
     CDS             42536..43096
                     /gene="yceB"
                     /locus_tag="N7C74_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU2430471.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVALTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            43202..44248
                     /gene="pyrC"
                     /locus_tag="N7C74_000532"
     CDS             43202..44248
                     /gene="pyrC"
                     /locus_tag="N7C74_000532"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HGU2430472.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILHAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEHQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            44322..44567
                     /gene="dinI"
                     /locus_tag="N7C74_000533"
     CDS             44322..44567
                     /gene="dinI"
                     /locus_tag="N7C74_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HGU2430473.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            44857..45111
                     /gene="bssS"
                     /locus_tag="N7C74_000534"
     CDS             44857..45111
                     /gene="bssS"
                     /locus_tag="N7C74_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HGU2430474.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            45226..46344
                     /gene="solA"
                     /locus_tag="N7C74_000535"
     CDS             45226..46344
                     /gene="solA"
                     /locus_tag="N7C74_000535"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HGU2430475.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDDLSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFSLSRFQ"
     gene            46392..46505
                     /locus_tag="N7C74_000536"
     CDS             46392..46505
                     /locus_tag="N7C74_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HGU2430476.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT        11228 a        12546 c        11586 g        11160 t
ORIGIN      
        1 gcgtgagggg tgtatgtagg gcgttcggta ggtctgataa gaggcgcatc aggcattgat
       61 gctcgactgt cggatgcggc gtgaacgcct tatccgactt acagaattcg gagcgcatca
      121 ggtaccggca aggttacctg atgcgacgag ggcatttacc cgagggtttt gaacgcttta
      181 atgcgctgca aatgcgggga gatattcttg aatttgtgcg tctgctcttc gtcccagaca
      241 atctcgtaat aatgatgcaa ctcttcagat gtccgctggc tgttaagcgc ttcatcatta
      301 cgcgacaaaa tcaccaggca acgatcgcga ttcttctcgc ggaagttggt cacacactta
      361 gtggcaatat cggcatactc ttccgggcga tcaatcttgc cttccatgtt ctcataaggg
      421 aacaaattag ggttgaagat cacctggcgg atatcgcaaa gaaaaccaat ccgttccgcc
      481 cagtatccgc ccagaccaac gccgcaaatc aggggacgct cgtcgacgtt cagttgcaac
      541 attttgtcca cttctttgag cagatgctgc atatcatgtt tcggatgccg cgtactgtag
      601 cttatcaagc gtacatccgg gtcaataaac tgcaattgta agactttctc gtggttaccc
      661 ggactgttag agtcaaaacc gtgtaaatag ataatcatcg cttcctcaca taagccaggg
      721 ttagtgacct gctttctctt cctgccagcg ttcatgtaac tgattcagac gggcgctgat
      781 cgctttccag cgagccgagt ccatcagttc ctgtcgcgaa aatgaacctt tatgatacaa
      841 acgtgtaaca cgttctgcct tgatcggtga cagattatct aacacgctga cggccccttt
      901 acgattattg cagaccagga tcatatcgca acccgcatcc agtgatacct gcccgcgttc
      961 ggcataactg cccataatcg cggcaccttc catcgataaa tcgtcagaga aaatcacgcc
     1021 gtcaaaaccc agttcctgac gcaaaacggt tttcagccag taggaagaac cgctcgccgg
     1081 acgcggatca acatcactgt agatcacatg cgcaggcata atggcgtcga gtttattttc
     1141 gcggattaac gtgctgaaaa ccgacatatc tttggcacga atttccgctt gcgggcgtgg
     1201 gtcgcacggc gtctctttgt gtgaatctgc agttactgca ccgtgtcctg ggaagtgttt
     1261 cccggtcgtt ttcattccgg cttcatgcat cccatcaata aaccgactgg cgattgccag
     1321 ggctttttct gggtcggcat gataagaacg ctcgccaatc gccgcgctga tatgtcctac
     1381 atccagcact ggcgcaaagc tgatatcaat atccatagcg atcatttcgc tggccatcag
     1441 ccaacccgcc tcttgcgcca gtttgccgcc ctcttccatt cccaacagcg cagcaaagga
     1501 ttgtgctgcc ggtaagcggg taaaaccttc gcgaaaacgc tggacgcgtc caccttcctg
     1561 atctaccgcc accaccagat gattgcgcga tgctgcgcgg atctggcgca ccagttcacg
     1621 caactgggca ggatcatgat agtttcgcgt aaagagaatc agccctccca ccagcggatg
     1681 cgccagtatt tcacgctctt ccgcgtccag ttcgtaacct tcgacatcca acattactgg
     1741 acccacactg ctctccttat tgttttatta atagctgccg ccaggtgtca tcggccagcc
     1801 tgataaattg ttgatcgccg gtttgtcgcc agcggtactc aaaccaccct gctttgagca
     1861 tcagcagcca gggaaaccat cgcctgacct gacgccataa ttgcgccgga taaatcttcg
     1921 cgcgagtggc atagtcattg accaattgcc ggtgctgttc agtattttcc acccacaccg
     1981 ccgccagttc cagcgcgata tcaccatctc ctgcatactc ccagtcgatg agttttaacc
     2041 ctgacgcgct atgcactaaa tttccggcgt ggacatccat atgcaatgga cttaagcgta
     2101 aaggccgtgg ttcccgcgct ttgcgcagac gttttaacat tcgcagccaa cccactgtcc
     2161 gccgcgccgg atcgctttgc tgccagtaca gttccagtaa cggcaacagc gttattcgcc
     2221 agccaaaacg tggttgttga tgtagataat acagcaagcc tgccagttcg ttggtatccg
     2281 gcaaatacgt ttttacctcg ccgggcagat agtcgactac catccagtca cggagatata
     2341 aatgcggctt cggtgcaatg catgcgggta gttgtgataa agcccgatac tggtgcaaga
     2401 acgcggactg cggcgcatca ggatcgtgcg gctgacgcac aacaaaacgc tggccctgat
     2461 gttcaatgag aaaactaccg ccactaagcc cactattaaa cgtcgtgacg ggatgaaact
     2521 gcgggaaaaa gcgcgacagc aattcgtcgc gcgtgatggg attattgctg cgaaacggca
     2581 cctttacctg accagataat ttcgcccgtc tgcaccagca tcagctgcat ttgtagggtc
     2641 ggagcgttaa cgttgccaga ggcgctggag tacagcacgt aatgagcgcc gacattgcgg
     2701 gcaatgccta tggctttact acgggtgcct aaactatcct gcggcgacaa acctaactgt
     2761 tgcttagcca tcgacagctg ctgggcggaa accagggtaa atttcccgtt attagccagc
     2821 gcatttcgca gcgtttcggt cgcttctgcg gcattcagcg aaccgttagt acggttgtta
     2881 acgctatcaa ccagcaggac gctacccgca gtcaccccgt cagccccaag catcttactg
     2941 accatcggct gcattgcgcc attccagtca taatggcgaa tatgcggcgc aggcggtgca
     3001 gtttgatctt cgtgctcaat tgggcctggc tgctgcggga tcgtcggcac cgagggcact
     3061 gtggggacag gctgttgtgg ctcggctggt tgttccggcg ctggtttcac ttcttctacc
     3121 ggtgcaggtt cacgttgccc cacacacccg gcgagaaaca tcgccagcgc ggtaatcaag
     3181 gcgtagcgac tcatttttgt catcaagatt caccccttac aaatatagat aaagtctgac
     3241 tttgtgcgcc cccagaaaat tggcgctgcc gtacagcgtt accgccgaat gtgcgggaat
     3301 ggtaacgctg cgtggccttt ccagaggatg catctccagc cctctggcgt cataccagta
     3361 aaaacgataa tgaacggtaa cgggttcttg cctttcgtta taaagcgttg aggatgctga
     3421 aggttgaata tcagacgtcg aaaggacggg cttttctgca ctgatgcccg cagccaataa
     3481 agatgactcc atcaccagcg attgctcatc attcaccgga atttccggat gtgaacgaca
     3541 gcccaccagc agtaacaacg ccagagacac cagcccaaag catccttttc tcatcgttac
     3601 agacctttat gcgccagcat tggtcccagc ggacggccac ccaacaagtg catatggatg
     3661 tggtaaacct cttgtccgcc atggcggttg gtgttcatga tcagacgata gccatcttcg
     3721 gcaatacctt cttgctcagc aatttttgcc gctacggtga tcatgcgtcc cagcgcctgc
     3781 tcatgctcag ctgagacgtc gttcacagtc ggaatgagga tattcggaat gatcagaata
     3841 tgcgttggcg cttgcggcga aatatcgcga aacgccgtta ccagatcatc ctggtagacg
     3901 atatcggagg ggatctcacg acgaataatt ttgctgaata tagtttcttc tgccacgaca
     3961 ttttcctttt tcaaatagcc atgcgttgcg ccatgctacg ccgcgccaca ctgcgagtat
     4021 agagtatggt cgagttaaac ctgctctttc aaccttaact cacgatttct taagcaaaaa
     4081 atcgttttgc tgccgtatat atctccatta ttcccatttc tgcctgcgaa tgcgctgaaa
     4141 agacattctc tgtttggttt acacgacaaa tatgaatgcg tatatttctc atttgcattt
     4201 gcatttgcat ttgcatttgc aagcaaaatt attcgcacac taaatataac cgctcatcac
     4261 cctcctccgg catgagcctg acgacaacat aaaccaagag atttcaaatg ctttcaacac
     4321 aatttaacag ggataatcaa tatcaagcca tcaccaaacc gtcactactt gccggttgca
     4381 tagcactggc actattacct tctgccgctt ttgctgcacc agccactgaa gaaacggtga
     4441 ttgttgaggg ttcagccaca gctccagatg atggcgaaaa tgattacagc gtaacgtcta
     4501 cctctgcggg taccaaaatg cagatgactc aacgtgatat tcctcagtcg gtcactattg
     4561 ttagccagca gcggatggaa gatcagcagt tacaaacgct gggcgaagtg atggaaaaca
     4621 cgctggggat cagcaaaagt caggcggatt ccgatcgtgc tctttattat tcccgcggat
     4681 tccagatcga taactatatg gttgatggta tccccaccta ttttgaatcg cgctggaatc
     4741 tgggcgacgc actttctgat atggcactgt ttgaacgcgt agaagtagtg cgtggcgcga
     4801 caggactcat gaccgggacg ggtaatccat ctgcggcaat taatatggtt cgaaaacacg
     4861 cgaccagtcg tgaatttaaa ggcgatgtct cggcggaata cggtagctgg aacaaagaac
     4921 ggtatgtggc ggatttacaa agcccactca ccgaagacgg taaaatccgc gcgcgaattg
     4981 tcggcggcta ccagaataac gactcatggc tggaccgcta caatagtgaa aagaccttct
     5041 tctccggcat tgtcgatgct gatttaggcg atcttacgac gctttcagcc ggttacgaat
     5101 atcagcgcat tgatgttaat agcccaacct ggggcggttt accgcgctgg aatactgatg
     5161 gcagcagcaa cagttacgat cgcgcacgca gtaccgcacc tgactgggcg tacaacgata
     5221 aagagatcaa caaggtcttt atgaccctga agcagcggtt tgctgatacc tggcaagcga
     5281 cactgaatgc cacccactct gaagtcgaat ttgacagcaa aatgatgtat gtcgatgcct
     5341 atgtaaacaa agcggatggt atgctggttg ggccatacag taattatgga cctggctttg
     5401 attatgtcgg cggcaccggt tggaacagtg gcaaacgtaa agttgatgcg ctggatttgt
     5461 tcgctgacgg tagttatgaa ttgtttggtc gtcagcacaa tctaatgttt ggtggcagtt
     5521 acagcaaaca aaacaatcgt tacttcagtt catgggccaa catcttcccg gatgaaattg
     5581 gcagtttcta caactttaat ggcaatttcc cacaaaccga ctggtcacca cagagcctgg
     5641 cgcaggacga taccacacat atgaaatcgt tatatgctgc cactcgtgtc acccttgccg
     5701 atccgctgca tctgatcctc ggcgcacgtt ataccaactg gcgggttgat acgctgactt
     5761 acagcatgga gaaaaaccac accacgcctt acgctggtct ggtgtttgac atcaatgaca
     5821 actggtcgac ctacgccagc tatacctcta ttttccagcc gcaaaatgat cgtgacagtt
     5881 caggcaaata tctggctcca atcaccggta acaactacga gctgggtctg aaatcggact
     5941 ggatgaatag ccgtctgacc accacgttag ccatcttccg tattgagcag gataatgtcg
     6001 ctcagtccac cggtacacct atccccggca gcaacggcga aaccgcctat aaagcggtgg
     6061 atgggacagt cagtaaaggg gtggaatttg aactcaacgg cgcaattacc gacaactggc
     6121 agctgacatt tggcgcaacg cgctatattg cagaggataa cgaaggaaac gccgttaatc
     6181 ctaatctgcc acgcaccacg gttaaaatgt tcaccagcta tcggttgcct gtcatgccag
     6241 agttgacagt cggcggtggt gttaactggc aaaatcgcgt gtataccgac accgtgacac
     6301 cgtatggcac cttccgcgcc gagcaaggta gctacgcgtt ggtggatctc ttcacccgct
     6361 accaggtgac gaaaaacttc tcgttacagg ggaacgtcaa taacctgttc gacaaaacct
     6421 acgataccaa cgtggaaggt tctatcgtct acggcgcacc gcgtaatttc agcattaccg
     6481 gcacgtatca attctgaata acacctgtaa aaaaggcagc catctggctg ccttagtctc
     6541 cccaacgtct tacggattag tggttacgga tgtactcatc catctcggtt ttcaggttat
     6601 cggatttagt accgaaaatc gcctgaacac cagaaccagc aaccactacg cccgctgcgc
     6661 ccagtttctt caggccagcc tgatccactt tagacacatc agcaacgctg acgcgcagac
     6721 gggtaataca tgcgtcgagg ttagtaatgt tttctttacc accaaatgca gcaaccagag
     6781 ccggtgccat ttcgctggta cctgtcgctt ttgcatcttc agtcgcgtct tcacgacccg
     6841 gcgttttcag atccagtgct ttaatcagca cgcggaagat ggtgtagtaa acaatcgcat
     6901 aaccgatacc gacgatcggg aacagccaca gtttgctgct gttaccagac agaacgatga
     6961 agtcgatcag accgtgcgaa aacgacgtac cgtcacgcat ccccagaaga atacagattg
     7021 ggaatgccag gcctgccaga atcgcgtgga tgatgtacag gatcggcgca acgaacatga
     7081 aggagaactc gatcggctcg gtgataccgg tcaggaacga ggtcagcgcc gcggagatca
     7141 taataccgcc cactttcgcg cggttttctg gtttagcaga gtgccagata gcaattgcgg
     7201 cagctggcag accgtacatt ttgaacagga agccaccaga cagtttaccc gcagtcgggt
     7261 cacccgccat ataacgcgga atgtcgccgt ggaaaacctg acctgctgcg ttggtgtatt
     7321 caccaatctg catctggaaa ggtacgttcc agatgtggtg cagaccaaac ggtaccaggc
     7381 aacgttcgat gaaaccgtaa atgccaaacg caactaccgg gttctggtaa gcagcccact
     7441 gagagaaggt ctggattgca gaaccaatcg gcggccaaat gaaggacagc acaacgccag
     7501 taaagatggc agccaggcca gaaatgatcg gcacaaagcg tttaccggca aagaagccaa
     7561 gatactcagg cagcttaata cggtagaaac ggttaaacat gtacgctgcg atcgcaccgg
     7621 agataatccc tccgagtacg ccagtatccg ccaggtgttt agaggcgatt tcttcagcag
     7681 gtaaatgcag taccagtggc gcaaccacgg ccatggtttt aaccatgatg ccataggcaa
     7741 caactgcagc cagcgcggat acgccatcgt tattggtaaa gccgagggcg acaccgatcg
     7801 caaaaatcag tggcatgttt gcaaagacgg aaccgcctgc ttctgccata acatgcgata
     7861 caacggcggg cagccagctg aaattcgcgg aaccgacgcc cagcagaata cctgcgatag
     7921 gcagtacgga taccggcagc atcagcgatt taccgacctt ttgcaggtta gcaaatgcat
     7981 tcttaaacat aattgagagt gctcctgagt atgggtgctt tttttacgtt ctcacgcgtg
     8041 gcaagggggg agagcctcgc cgtgtacagg gcatctaagc gccctttatt tattacacag
     8101 agtaaaataa ttcagtgcca atttgtttga cggctatcac gtttcaactt cagatcattg
     8161 gagaagttgc acattttgac aaaacatatg tcacaatatt tcaaacgtgg ttattcaccg
     8221 cccaattttc ggcggtgaac tttactcgtt ttagtcatta attacgagct ttaaaaaaac
     8281 tcattcaacg gatggattga aggcgggaag cgtcgatgtg aaacagacgg gcgaagttat
     8341 cggtggttac ctgcgccagt tcttcaacgg caacaccttt caacacagcc atgtattctg
     8401 caacgtcacg aaccatcgca ggttgattct cttttcctcg atgcggcacc ggcgcaaggt
     8461 aaggtgagtc agtttccacc agtaaccgat ccagggggac ataacgcgca gcatcgcgca
     8521 gttgctccgc attacggaag gtcacaatgc cggaaaagga gatgtaaaat ccgagatcca
     8581 gtaatttgcc cgccgtttct ctgtcctctg taaaacagtg tagtacgcca ccgcaatccg
     8641 tcactttttc ttcgcgcaga attgccagcg tatcggcgcg ggcgtcacgg gtatggacga
     8701 taaccggctt gttcagttca cgaccaatct ggatatgatg gatgaaggac tcttgctgac
     8761 gtactttagt ttccggtgtg taataataat ccagcccggt ttcacccagc gctacaacac
     8821 cctcttctgc cgccagacgg cgtaaatctt ctacatcgta gggatcattc tggttgagtg
     8881 gatgcacgcc acaagaaaat acgacgttgt cacgttcgcc taccagatcc cgcatgtgta
     8941 aataacccgg taatgttgtg gcgactgcca gacaaaattt cacatcgcgt gcggcggctt
     9001 tcgccagaac gtcatccacg tccttatgca aagattcata atccagacca tcgagatggc
     9061 agtgtgagtc gactaaaaac atgatgtctc tcttaaagat gaggaaccgg tagcacaacg
     9121 cccggttgca ggtaatgctc aatacgcagt aaaagatcgg tgatgagaag ctcgcggttg
     9181 atgcctgtga cagacattaa ctgttcacga atgtggcaaa catcccccag tatagcctgc
     9241 aggcgcgagg gagaaagatg gtttgccagt tcggcgacca ggcccggcac atcaacattg
     9301 gtcacctgcg cagcgccatg atggcgtttt agcgcatcca tcagcaacgt tgccagccag
     9361 tgtaaacgcg ccggagcttg ttcatgatta agggccgcca gcagcgaata ccagtcgccc
     9421 gatggcacgc tatatgccaa cgcctgacac aatgtttcac gagcctgcca gttatctccc
     9481 tgaaacaacg ccagtgccgc gccaggcgaa ccggcgctta agcgcaatgc ggcaagtaat
     9541 gcatcctgtg acattgtcac ttcgcgtgaa agccaggtca cggcgtactg ttccggcggc
     9601 ggcgcaaggt aatgtaaccg acaacgacta cgtaacgttg ccagtaaacg ttcaggctcg
     9661 cgggtagcca ggaaaaacca ggtttctgct ggtggctctt caagcgtttt cagcaatgcg
     9721 ttagccgcgg cgtcggtcag taaggcagca tcggttaccc agacaacttt cgcaccacct
     9781 aagcgtgcgt gctcattcag cttttcggtg acctcacgta ccgcatcaat gcccagcgta
     9841 tttttccctt tttcgggagc cagggtgtag taatcgggat gcgtgccagc ctgcatcaac
     9901 tgacatccac gacagtgacc gcaacttttg tggccctgcg gttgttggca gagtaaataa
     9961 cggctcaggg cgtagattaa agcatcatcg cccatgcccg gtaacgcctg aatgagtagc
    10021 gcatggtgac ctcttccggc ctgatagctg gctaccagtt tttcgaaatc aggtcgtaac
    10081 catggatacc atctcatgcg tccaactcct tcacccagcg ggtcacggta gtgcggattg
    10141 catccatcac ggcctccagc ggctgggtgg catcaatggt atgaatactt ttatcttgtg
    10201 cagccagttc cagatagcgg gcgcgggtgc gattaaagaa atcgaaagac tcttgctcaa
    10261 tacgatccag ctcgccgcgc gcacgcgcgc gttttaagcc aacttccggg gtaacatcga
    10321 gatagagcgt taagtcgggg cgaaaatccc cgagaacagc atcacgcagt gttgccagca
    10381 tatgttggtc aataccacgt cctccgccct gatacgcctg agtggagaga tcgtggcgat
    10441 cgccaataac ccaggtgccg ttagccagcg caggtttgat gaccgtttct accagttgaa
    10501 cgcgcgcggc ataaaacatc agaacttcgg ctttatcggt aatgatttca tcgcctaccg
    10561 atttgatatc cagcaccagg cttcttaact tttcggcaag ttgcgtacca ccaggttccc
    10621 gagtgaaaac catgtcgcgg atacccagtt gctcgagcgt ctcaaccacc acattacgcg
    10681 cggtagtttt gcctgcgcct tccagcccct caatgacgat atacttactg cgcatttttt
    10741 tccttaagca ctttcagata atcctgcaca gacttgttat gactggcaag attggtatta
    10801 aacgtgtgac cacctttacc atcggccaca aaatagagat acggcgtttt tgccggatgc
    10861 gcagcagcct tcagcgaatc cgcccccggc gtagctatcg cacccggcgg caaaccggta
    10921 atggtatagg tgttatacgc tgtcggcgtt tccaggtctg cacgagaaag tttgccatta
    10981 taacgctctc ccatcccgta aatcacggtt gggtcggtct gcaggcgcat accaatgcgt
    11041 aaacggttga taaataccga ggcaacctga tcgcgttcac tggcaatggc ggtttctttt
    11101 tcgataattg atgccatcgt caccagctga tttttatctt tataaggcag accgtccgca
    11161 cgcccttccc aggcgctatc gaccgctttc accattttct tgtgcgctcg cttgagtaac
    11221 gcgacatcgg tggtattggc ggtatacatc caggtgtctg gccagaacca accttcaatc
    11281 cactccgggt tttccagttc aagtgcctgc gctacggtga tgtacttatc atcgctcagc
    11341 gtgtgcttga tatacggggc ctcacgcaat tgcttgaggt agtcgctcag acgcatccct
    11401 tctaccagtc gcagagggaa ctgtgcttct ttaccgcttt ccaacaattt cagcatctcg
    11461 cgcacggtca tctgcggtgt aaagcggtaa gtcccggctt taaagtgaga aagatccggt
    11521 tcgatacgca gcaaccattg aaaaacccgt gggcgattga tgatcttatc ggcataaagc
    11581 tgttcaccaa gcgccagacg tccggtccct ggcttcaggg taaatatcgt ctcttctttg
    11641 ataagcaatt tgctgtcggc aagatagcga accttccaga cgcccacacc agcggcgata
    11701 cccagtacca ccagcaataa caagattatc aataacactt ttttcatgac taattcgggc
    11761 gctcacaaag tggggctaaa tattcatata acgttgctga cgaaaaggag acatcgccac
    11821 aggcacgtac gggcatcact ggcattaacg cattacaaat aaccatctca tctgcctgca
    11881 acgcctcttc cagagaggct tgcacttcga caagctgata agaggattgt gccagcaaac
    11941 ggatacagaa ttgtcgcata atgccgttaa cacctgcctg atccagtcgc ggcgtataaa
    12001 ctacgttgcc cttccgccag aacaaattag ctgcacagca ttccgtaacc cccccttcgc
    12061 tgtcaaggac cagcgcctca tcagcgtttg tctgctcaag atgagagcgg atcaatactt
    12121 gctcaagccg attaagatgt ttaatacctg caagatgagg attgcgcccc agccgcaccg
    12181 ggcttagcgc caacgtgatc ccttcgttac gcaaacggtc gtaatgtgca ggataagccg
    12241 taacggagag aatccgcgtt gccggtccac tgttcaatgt gctgtaccct cgcccgccac
    12301 taccgcgact gatcacgact ttcagtacac cattttgctg ttctgctgcc agcgttttca
    12361 tctcttgttc aagctgaggc cagaagtcac aggaaatcat caaccgctga caagcatcct
    12421 gtagtcgctg gatatgcgcc gataacaaac tgactttacc gtcgataact ctggcggtgg
    12481 taaaacaacc atcaccaaac tgcgttgccc gatcgcttac tgacagcgat tcctgcttat
    12541 aaccgttaat taagaacata ccggctcctt atcagagaat tgttagtgtg gcagcatgtt
    12601 cactacggaa caagtcggaa taaaagctaa gaaaaaaggc ccgcaagcgg accttttata
    12661 tgggtggaaa atgacaactt agatcttttt aaagatcaaa gaaccattag tgccaccgaa
    12721 gccgaaggag ttacacagcg tgtattccat tccgctgacc tgacgtgctt catgcggtac
    12781 gaaatccaga tcgcaacctt catccgggtt atccaggttg atggttggcg gaacagcctg
    12841 atcgcgcagc gccaggatgg agtagataga ttctactgca cccgccgcac ctaacaggtg
    12901 accggtcata gatttcgtgg agcttaccaa cacacggctt gcagcttcac cgaagatggt
    12961 tttcaccgcc tgcgcttcag ctttatcgcc agccggcgta gaagtaccgt gcgcgttaac
    13021 gtagccaatc tgactcgctt caatgcctgc atcacgcaga gcatttgcca ttgccagagc
    13081 tgcgcctgcg ccattttctg gcggtgacgt catatgataa gcatcgctgc tcataccaaa
    13141 gccgacgagt tcagcgtaaa ttttcgcacc gcgttttttc gcgtgttcgt actcttcaag
    13201 taccagcata ccggcaccat cgcccagtac gaaaccatca cgctctttat cccacgggcg
    13261 gctcgccgct tgcgggttat cattgcgggt agataatgca cgtgccgcgc caaaaccacc
    13321 aacacccagc ggcgtactgg ctttctctgc gccacctgca accatcacgt cagcatcgcc
    13381 atacgcgata atacgcgcag catggccaat gttgtgcacg ccggaagtac aggcggtcgc
    13441 gatagagatg cttgggccac gcaggccata catgatagtc agatgacctg ccaccatgtt
    13501 cacaatcgtt gacggaacga agaatgggct gatcttacgt ggaccaccgt tcatcagaga
    13561 tgtgtggttt tcttcgatca gtccgaggcc gccaatcccg gagccaattg cggcaccaat
    13621 gcgggttgcg ttctcttccg ttatttcaag gccagaatcc tgcatggcct gaacgccagc
    13681 gacaattcca tattgaatga aggcatccat cttgcgctgt tctttgcgcg agataatgtc
    13741 ctcacagtta aaatccttta ctaagccagc aaatttcgtt gcataggcgc tagtatcgaa
    13801 atggtcgatt aggctgatgc cactctgacc ggcaagcaga gctttccagg tagactctac
    13861 ggtattgccg acaggagaca acatgcccag tccggtcaca actacacgac gcttagacac
    13921 gtttgtcctc cagggaggga aaaaatgatt ctagtgggac aaaaagataa aactcaggcg
    13981 gtcgaacgac cgcctggaga tgttcactta cgcctggtgg ccgttgatgt aatcaatggc
    14041 agcctgaacg gtggtgattt tctcagcttc ttcgtccgga atctcagtat caaactcttc
    14101 ttccagagcc attaccagct caacggtgtc aagagaatcc gcgcctaggt cttcaacgaa
    14161 agaagcattg ttggtaactt cttcctgctt aacgcccagc tgttcgccga taattttctt
    14221 aacgcgttct tcgatagtgc tcatactctt aaatttccta tcaaaactcg ctttcgcgat
    14281 ggttttcgta gtgtataaaa tgttgaaaaa tttgcaacta aatcccggca ggtcttacca
    14341 cgattttacg ttattttgag gccttacccc ccaataacgc aaatcatttt gcactaatta
    14401 ttactcataa ccacgcagac tgcgcgcaac atgagcattt tgtgcaaatc gcggtcagac
    14461 catgtacatc ccgccgttca catgcaaagt ttcacccgtg atgtaagctg cttcgtcgga
    14521 tgccaggaat gcaaccgcgt tggcgatttc ctgtgcgccg ccgaggcgac ccgcaggaac
    14581 ctgcaccagg atacccgcac gctggtcatc gctcagcgca cgtgtcatgt ccgtttcaat
    14641 aaagcccgga gcaacaacgt ttacagtaat accgcgtgac gcaacttcgc gcgccagtga
    14701 tttactgaag ccgatcaagc ccgctttcgc cgcagcgtag ttggcctgac cgccatttcc
    14761 catggtacca accacagaac cgatagtgat aatacgacca tgacgctttt tcatcatagc
    14821 gcgcattacc gcttttgaca gacggaaaac agatgaaagg ttggtttcga taatatcgtt
    14881 ccactcttca tctttcattc gcattaacag gttatcacga gtgataccgg cattattgac
    14941 caggatatcc acttcaccaa attctgcgcg aattttttcc agaacagatt cgatagatgc
    15001 cgggtcggtc acattcaaca tcagaccttt gccgttagca cctaaataat cactgatcgc
    15061 ctgagcgcca ttttcactgg tcgcagtgcc aataactttc gcgccacggg ctgcgagcgt
    15121 ttcagcaatt gcgcggccaa ttccgcggct tgcaccggtt accagtgcga tttttccttc
    15181 aaaattcatg atgttcctct tttaaagctc gagcgccgct gccatcgctg aaggttcgtt
    15241 cagcgccgag gcggtcaggg tgtcgacaat gcgtttcgtc aggccagtaa gcactttgcc
    15301 cgggccgact tcatagagat gttctacgcc ttgcgctgcc atgtactcaa cagacttcgt
    15361 ccactgaacc gggttataca actgacgtac cagtgcgtca cgaatggcat caccattggt
    15421 ttcgcatttc acatcaacgt tattcacaac aggaactgtt ggtgcgttaa aggtgatttt
    15481 cgctaattct actgccagtt tgtcggctgc tggtttcatc agcgcacagt gagacggtac
    15541 gctcactggt aacggcagag cgcgttttgc gcccgcagct ttacaggcag cgccagcacg
    15601 ctcaaccgct tctttatgac cggcaataac cacctgtccc ggagagttaa agtttaccgg
    15661 agaaacgacc tgaccttctg cagcttcttc acacgctttt ccaatagacg catcatccag
    15721 accgatgatt gccgccatag cgcccgttcc ttccggtacg gcttcttgca tgaacttgcc
    15781 gcgcatctca accagacgca ccgcatcagc gaaatcaatc acaccagcgc aaaccagcgc
    15841 ggagtattcc cccaggctgt gaccggccat cattgccggt gctttaccgc catgctgctg
    15901 ccatacgcgg tacagcgcaa cagaggcagt caacagcgct ggctgggttt gccaggtttt
    15961 attcagttct tcagctggcc cctgctgggt cagcgcccac aggtcgtagc ccagcgccgc
    16021 agaagcttca gcaaacgttt cttcgacaat tggatagctc gccgccatat cagccagcat
    16081 tccaacggtt tgagaaccct gtccagggaa cacaaatgca aattgcgtca tgttttaatc
    16141 cttatcctag aaacgaacca gcgcggagcc ccaggtgaat ccaccgccaa aggcttcaag
    16201 cagaaccaac tgccccggct tgatgcgccc gtcgcgtaca gcttcatcca gcgcgcacgg
    16261 gacagaggcc gcagaggtat taccgtggcg atccagcgtc accacgacat tgtccataga
    16321 cataccgagt tttttcgccg ttgcactgat aatacgcagg ttagcctgat gcggaaccag
    16381 ccagtccagt tgagaacggt caagattatt cgccgccagc gtctcatcaa cgatgtgcgc
    16441 cagttccgtt accgcaacct tgaagacttc gttgcccgcc atcgtcagat gaattgaatt
    16501 ctctggattc acgcggtcgg cgtttggcag cgtcagcaat tcaccataac taccgtcggc
    16561 atgcagatgg gtggaaatga ttcccggctc ttcagaggca gccagcaccg cagcgcccgc
    16621 gccatcgcca aaaataataa tagtcccacg atctgttgga tcgcaggtgc gcgccagtac
    16681 atcggaaccg acgaccagag catacttcac cgccccagat ttcacgtatt gatcggctac
    16741 gcttaatgca taggtgaaac ctgcgcaggc tgctgcaacg tcaaatgccg ggcaaccttt
    16801 aatgcccaac atgctttgaa tctgacaagc tgcgctcggg aaagcgtgcg tagcagaagt
    16861 cgttgccaca acgatcaggc caatctggtc tttctcaatg cccgccatct caattgcgcg
    16921 tgtcgccgct tcaaagccca tggttgaaac ggtttcgttt ggcgcggcaa tgtggcgttc
    16981 gcggataccg gtacgagtga caatccactc gtcagaggtg tccaccattt tttccaaatc
    17041 ggcgtttgtc cgcacttgtt cgggcagata gctgccagta ccaataatct tcgtatacat
    17101 gtacgctcag tcacttttcg gttatatacc gtcacttgca aactgcgagt tcgctggcag
    17161 cgtcctgcta ccgcagagtt ccgcttttgc caccgtccag cagctcaaaa ccagctgggt
    17221 atacagattc caggcgagcg gcaattcgct gaggaacttg tcgctgcacc gcctgcactg
    17281 cctgttcaat cgcgaccgca aaagctcgct gattggctgc accatgactt tttatcaccg
    17341 tgccgcgcaa tcctaacaga caggcgccgt tatactggtc ggggttgagg tgactgaatc
    17401 gcctcgtcag gctcttttgt agccaacgct ttaataacag tagccaccac gaccgttttt
    17461 tcccttcacc ctgagatttc agcagagaaa ggaacatcct gacaacacct tccatcgtct
    17521 ttaatgtgac atttcctgta aagccgtcac aaaccagcac atctgtcttg ccagttaaca
    17581 actcattggc ttcaagatag ccgatataat tgatagaagg gattgtttta agcaccgctg
    17641 aggcatcccg aatactatcg agacccttta cttcttcttc accaatattg agcaacgcca
    17701 cgcgaggatt gggaatttcc accacctctt cagccaggac tgagcccata atggcaaatt
    17761 gcaccaacat tgtgctgtca caatcgacgt tggcccctaa gtcaaggacc accgttttgc
    17821 ccttttgctg atgtggtaat accgtcacca gcgccggacg ctcaatcccc tccaggggct
    17881 tgagtaataa ttttgccagc cccatcagtg ccccggtatt accggcactg acacaggctt
    17941 gcgctcgacc ttctttcacc agctccaggg ccacgcgcat tgaactccca cgactggcgc
    18001 ggatagcttg cgaaggccgg gcatcactgg cgataactga ctgcgcagga ataatctgca
    18061 gacgcgaacg ttgttcaaag tcagctttag caagtaatgg cgtgatggcg tcgggattgc
    18121 cgactaaaag aagagtgagt tgcgaattag aattcagtgc ctgcaatgct gcaggcactg
    18181 tcacggaagg gccaaaatcc cctcccatga catctaacgc cagggttaga cgtgtcaagg
    18241 tatcgtcgcc gcccggtttg gtctttcccc agttgcctgg ggaaaatcct cactaagctt
    18301 catcacgcag acgcgtgatt acttagcgat gaccttgcgg ccgcggtagt aaccgtcggc
    18361 agtgatgtgg tgacgcaggt gtttttcacc agaagttttg tctacagaca ggctggtgac
    18421 tgcggtcagc gcgtcatggg aacgacgcat gccacgtttg gaacgggttg gtttattctg
    18481 ttgtacggcc atggacctta ctcctcaatt acggtttatc cccgatccaa cggggagcac
    18541 caattactta cgctttaagc tggctaatac ggcaaatggg tttggctttt gcgcttcttc
    18601 aggcagttca ccaaagacca tgtccgcttc ggacacttca cagtgttcag aatcatgcac
    18661 cggaactacc ggcaaggcga ggatgatttc atcttcaacc attgcaagca gatcgatttc
    18721 accgaattcg ttaacctcaa tcggttcata cgcttccggc agtgcttcag cctgttcgtc
    18781 tgaacgcaca ggactaaaac aatacgttgt gtagacctga tgagtaaacg gcttcccgca
    18841 acgctgacac tcgagcgtta ccgtcacctt cgcatcgccg tttaacactg cgagacgttg
    18901 gttatcgata gcgaacgaca tggagcattc cacatcactg tccacactga ctacggattc
    18961 ggcgacgcgc tcaacctgat caggagtata gataccctgg taatcaaggc gtttttgagc
    19021 cgtacgaacc ggatcgagag tcaggggtaa ttttaccttt tgcatagggc gcgcatatta
    19081 actttgtaac gtcatagagt caaagaaaaa ggcagcctgt ggttgccttt tgccaataat
    19141 tcgcacacat tgcgggttac tgctttattt tcgttcagtg ctgataacga acatcaacct
    19201 gcaaaacgaa gcgcatagtt taaaatggct atcatcaata cgctatatct gatggaaaaa
    19261 aatatgccta aacttatttt agcctccacc tcgccctggc gtcgcgctct tctggaaaaa
    19321 ctgcaaatct cttttgaatg tgcagcaccc gaggtcgacg aaactccaca cagcgacgaa
    19381 tcaccgcgac agttggtgct tcgactggca caagaaaaag cgcaatctct ggcgtcacgt
    19441 tatccggatc atttaattat tggttcagac caggtatgtg ttcttgatgg cgaaatcacc
    19501 ggtaaaccgt taacggaaga aaatgcccgt ctgcaattac gcaaagccag cggcaatatc
    19561 gtcactttct atactggact ggcgttgttt aattcggcga atgggcatct gcaaacagaa
    19621 gtggagcctt ttgacgtcca tttccgtcac ctgagcgagg cggagattga taattacgtg
    19681 cgtaaagagc atcccctgca ctgcgcgggt agctttaaga gtgaaggatt tggcattact
    19741 ctgtttgagc gtttagaggg gcgtgatcct aacacgctgg ttggtttacc gctaatcgcg
    19801 ctgtgtcaga tgttacgtcg ggaagggaaa aaccgctgat gggatagttt tttgcgccgg
    19861 atgcggttac gtcgcatccg gcaatcggtg ctggcggcct gatgcggcgt aaacgcctta
    19921 tcccattatc atgcttatat gctcgtatta gcgcgcgtta cgcagctttt gcaaacaacg
    19981 cttcaaacca tcatccatcg gcgcttcgat acgcatcacc tcaccggtcc ccggatgggt
    20041 aaacttcaac gctgcggcgt gcaggaacag acgatttaat cccgtgcctg cttcagtgag
    20101 ctgtctgtca aattcacggt caccgtagcg atcgtcaaag gcaatcggat gacctgcata
    20161 ctgtgtatgc acacggatct gatgagtgcg ccctgttacc ggactacaac gcaccagggt
    20221 ggcaaatgca tagcgttctt ccactttaaa gcgtgtttcc gacggtttgc cttcctgact
    20281 cacacgcacg atacgttcgc cgctttgcag aatatttttc aataacggcg cctgaacgct
    20341 cttcacatgc gactgccact gaccgcgcac cagcgccagg taatcttttt gcatcccttt
    20401 ttcacgtaat tgctcatgca gagaacgcaa cgccgagcgt tttttcgcta ccagcaacac
    20461 acctgaggtg tcccggtcaa gacgatgaac cagttcaagg aaccgcgctt ccgggcgcaa
    20521 cgcccgcaaa ccttcaataa cgccgaagct taaaccactg ccgccatgta ccgccgtacc
    20581 ggaaggttta ttcagcacca ggatgtgatc atcttcatat aagatgacgt ccgccagcgc
    20641 cgccaccttt tgcagatgtg gcgaaaccgc ctcttcttcc cgctcagcaa cgcgaaccgg
    20701 tggaatacgc acctcatcac ccgcttcgag tttatattca ggcttaatac gttttttgtt
    20761 cacccgcact tcgcctttac gcaaaatacg gtaaatcata ctttttggta cgcctttcaa
    20821 ttgggtacgc aaaaagttgt cgatacgttg ccccgcttcg tcggcggtga tagcaacaat
    20881 ttttacggat ggagtctctg ttttcatggt gggcgattct aattagccaa caggattcgc
    20941 gccactcatt tttctatgct tatatttagt tttgcacctt attacttcac tacgtgatca
    21001 ctttattgat ggttattaaa ccaatcacca gcaagaagtg aaaaaactgt gagtaagcgg
    21061 gtgataaatg gtaaaagtca tcttgctata acaaggcttg cagtggaata atgaggccgt
    21121 ttccgtgtcc atccttgtta aaacaagaaa ttttacggaa taacccattt tgcccgaccg
    21181 atcatccacg cagcaatggc gtaagacgta ttgatctttc aggcagttag cgggctgcgg
    21241 gttgcagtcc ttaccggtag atggaaatat ttctggagag taatacccag tctgtttctt
    21301 tgataattgc gctgtttttc cgcatgaaaa acgggcaacc gacactctgc gcctctttga
    21361 gctgacgata accgtgaggt tggcgacgcg actagacacg aggccatcgg ttcacacccg
    21421 gaaaggcgtt actttgcccg cagcttagtc gtcaatgtaa gaataatgag taagttacga
    21481 tgaaaagaat gttaatcaac gcaactcagc aggaagagtt gcgcgttgcc cttgtagatg
    21541 ggcagcgtct gtatgacctg gatatcgaaa gtccagggca cgagcagaaa aaggcaaaca
    21601 tctacaaagg taaaatcacc cgcattgaac cgagtctgga agctgctttt gttgattacg
    21661 gcgctgaacg tcacggtttc ctcccactaa aagaaattgc ccgcgaatat ttccctgcta
    21721 actacagtgc tcatggtcgt cccaacatta aagatgtgtt gcgtgaaggt caggaagtca
    21781 ttgttcagat cgataaagaa gagcgcggca acaaaggcgc ggcattaacc acctttatca
    21841 gtctggcggg tagctatctg gttctgatgc cgaacaaccc gcgcgcgggt ggcatttctc
    21901 gccgtatcga aggcgacgac cgtaccgaat taaaagaagc actggcaagc cttgaactgc
    21961 cggaaggcat ggggcttatc gtgcgcaccg ctggcgtcgg caaatctgct gaggcgctgc
    22021 aatgggattt aagcttccgt ctgaaacact gggaagccat caaaaaagcc gctgaaagcc
    22081 gcccggcccc gttcctgatt catcaggaga gcaacgtaat cgttcgcgca ttccgcgatt
    22141 acttacgtca ggacatcggc gaaatcctta tcgataaccc gaaagtgctc gaactggcac
    22201 gtcagcatat cgctgcatta ggtcgcccgg atttcagcag caaaatcaaa ctgtacaccg
    22261 gcgagatccc gctgttcagc cactaccaga tcgagtcgca gatcgagtcc gccttccagc
    22321 gtgaagtccg tctgccatcc ggtggttcga ttgttatcga cagcaccgaa gcgttaacgg
    22381 ccatcgacat caactccgca cgcgcgaccc gcggcggcga tatcgaagaa accgcgttta
    22441 acactaacct cgaagctgcc gatgagattg ctcgtcagct gcgcctgcgt gacctcggcg
    22501 gcctgattgt tatcgacttc atcgacatga cgccagtacg ccaccagcgt gcggtagaaa
    22561 accgtctgcg tgaagcggtg cgtcaggacc gtgcgcgtat tcaaatcagc catatttctc
    22621 gctttggcct gctggaaatg tcccgccagc gcctgagccc atcactgggt gaatccagtc
    22681 atcacgtttg tccgcgttgt tctggtactg gcaccgtgcg tgacaacgaa tcgctgtcgc
    22741 tctctattct gcgtctgatc gaagaagaag cgctgaaaga gaacacccag gaagttcacg
    22801 ccattgttcc tgtgccaatc gcttcttacc tgctgaatga aaaacgttct gcggtaaatg
    22861 ccattgaaac tcgtcaggac ggtgtgcgct gtgtaattgt gccaaacgat cagatggaaa
    22921 ccccgcacta ccacgtgctg cgcgtgcgta aaggggaaga aaccccaacc ttaagctaca
    22981 tgctgccgaa gctgcatgaa gaagcgatgg cgctgccgtc tgaagaagag ttcgctgaac
    23041 gtaagcgtcc ggaacaacct gcgctggcaa cctttgccat gccggatgtg ccgccagcgc
    23101 caacgccagc tgaacctgcc gcgcctgttg tagccccagc accgaaagct gcaccggcaa
    23161 caccagcaac tcctgcacaa cctgggctgt tgagccgctt cttcggcgca ctgaaagcgc
    23221 tgttcagcgg tggtgaagaa accaaaccga ccgagcaacc agcaccgaaa gcagaagcga
    23281 aaccggaacg tcaacaggat cgtcgcaagc ctcgtcagaa caaccgccgt gaccgtaatg
    23341 agcgccgcga cacccgtagt gaacgtactg aaggcagcga taatcgcgaa gaaaaccgtc
    23401 gtaatcgtcg ccaagcacag cagcagactg ccgagacgcg tgagggccgt cagcaggctg
    23461 aggtaacgga aaaagcgcgt accgccgacg agcagcaagc gccgcgtcgt gaacgtagcc
    23521 gccgccgtaa cgatgataaa cgtcaggcgc aacaagaagc gaaggcgctg aatgttgaag
    23581 agcaatctgt tcaggaaacc gaacaggaag aacgtgtacg tccggttcag ccgcgtcgta
    23641 aacagcgtca gctcaatcag aaagtgcgtt acgagcaaag cgtagccgaa gaaacggtag
    23701 tcgcaccggt agctgaagaa actgtcgctg ccgaaccaat tgttcaggaa gcgccagctc
    23761 cacgcacaga actggtgaaa gtcccgctgc cagtcgtagc gcaaactgca ccagaacagc
    23821 aagaagagaa caatgctgat aaccgtgaca acggtggcat gccgcgtcgt tctcgccgct
    23881 cgcctcgtca cctgcgcgtg agtggtcagc gtcgtcgtcg ctatcgtgac gagcgttatc
    23941 caacccaatc gccaatgccg ttgaccgtag cgtgcgcgtc tccggaactg gcctctggca
    24001 aagtctggat ccgctatccg attgtacgtc cacaagatgt acaggttgaa gagcagcgcg
    24061 aacaggaaga agtgcaagtg cagccgatgg tgactgaggt ccctgtcgcc gccgctgtcg
    24121 aaccggttgt tagcgcgcca gttgttgaag aaatggctga agtcgtagaa gcccccgtac
    24181 cggttgccga accgcaaccg gaagtggttg aaacgacgca tcctgaagtg attgccgccg
    24241 cggtaactga acagccgcag gtgattaccg agtctgatgt tgccgtagcc caggaagttg
    24301 cagaacacgc agaaccggtg gttgaaccgc aggaagagac ggcagatatt gaagaagttg
    24361 ccgaaactgc tgaggttgtg gttgctgaac ctgaagttgt tgctcaacct gccgcgccag
    24421 tcgtcgctga agtcgcagca gaagttgaaa cggtaaccgc ggttaaacca gagatcaccg
    24481 ttgagcataa ccacgctacc gcgccaatga cgcgcgctcc ggcaccggaa tatgttccgg
    24541 aggcaccgcg tcacagtgac tggcagcgcc ctacttttgc cttcgaaggt aaaggtgccg
    24601 caggtggtca tacggcaacg catcatgcct ctgccgctcc tgcgcgtccg caacctgttg
    24661 agtaataatt agctcaagta atcaagccct ggtaactgcc agggcttttt tatttcatct
    24721 ttgaatcaac atattaaaat tcatcgaatg gcatccttgc taaccaacaa tgcaaaatag
    24781 gcaaaaaaat gcccgccccg gcgatccctg agcgggcaga caaaacatac ccagtttcat
    24841 gatatgtttt aaagcgagct tatttgttga gctggaagag cgacaatccc tgcatatcgg
    24901 taaatgcttt atacgatgcc tgcaatgccg tttgctgcat gatgtaagat gaaatagttg
    24961 cattccagtc cacatcaacc agatcgctca tctgctgcgt ttgccctaat gcgcgatcgc
    25021 tacctaatga atccagcgac tccagttcgt tcagctgcgt gcctaattcc gcgcgaacgg
    25081 tcaggacatt gttcagcgag tttttcagtc cgcggttggt tttatctaac gccgcagcgg
    25141 cggtttcttt atccgcttcg ctatccgcga ccggcgtttt cagggctgcg atggcgctat
    25201 ccagcatggc aaaaagattg gtttcagaag cgctaccgtc tggttccgct accgcgttac
    25261 tggtaatact gtcgaaaatt ttgtcgcccg tatgtcctat caccatcgaa cgcgaagcat
    25321 cgacctgttg cttaatactt tctgttccgc cgacataatc accgttaacc tcactaaacg
    25381 gcgcagtctc tgttttataa ccggcaaaaa tgtagcgccc gttaccgtca gtggtgttcg
    25441 ccagattcag caactggtca cgaagcccct gaatatccgt agccagcgag gcccggtcat
    25501 tgtcactcaa ggtgccattg ctggcgtaga caattttttc ctgagcattc tggatagcgg
    25561 tggtgacctg gctaagtaca ctctcttcca gtgacacttt ttgagtggcg aaagtacgtg
    25621 ccagcgtgta ctggctgttt tgcgcctgtg cctgggagag aacaacagct tgtgatgcag
    25681 caatgggatc gtctgaaggg ttaacgactc gcttacccgt cgacatctgc tcgccgtact
    25741 tcatccattc tgcctgagaa ttggtgatgc cgcgcatgtt ttgctggtac atcatctggg
    25801 tactgaaacg cattttatct ccccttagcg aatgttaatc agcgcatcaa aaatcgcgtt
    25861 tgccgtctgc agaacctgcg cattcgccag gtaatactgc tgaaaacgtt gcagatttcc
    25921 gtactcctca tcgagattga caccggaaat cgactgctgc tgattggaaa gctgcgtcac
    25981 cacattacct tgcgtggtgc tactggtttt caacgtcgcg gttttattac cgatatcact
    26041 cactaacgag gcataagcgt cgttaaagga tttcgctccg cccaccgttt tactgttgct
    26101 ttgcagatcc agcagggcct gaccgttgcg gttgtcgcta tcacccgcat cttcttcgct
    26161 cgccatcgct attttcgctt cgtcggtgat taatacatcc atgttgacga tggcgtcact
    26221 tactggtttc agcgtgaagc tgtcgttaac ggcaggcgtt cctgtaaacg tcaactccag
    26281 accatcaaat gccactttac cgttggcatc tggcgtcacc gtaaaagtgg tattgctggc
    26341 aaggcgggtg acctgccact gattattatc gaacgagatt ttgtaatctg tcgccagtac
    26401 cgcggaggca tcagttaccg tggcaccgat cgcaacgtta ccgttgtttt tagtgttttg
    26461 cagaaccgcg ggcttaccga tagcaaagaa atcttcaccc tcatcgccgt tggcatcaaa
    26521 tccggctttg tgttggctgt tgaaagcctc agcaaatgcc agcgccagtt gtccaagcgt
    26581 attacgcgtc tggtccagat cctgagaacg gaatgtcaga atgccgccca gcgacccggt
    26641 attcagtaat ttctccggga tctcaatatt gcctgccgtc ccatcaacat aagcgacagt
    26701 cgtacgagaa gggtcggcgc tggaaggaac tgccgccagt tgccgcgccg tacttccctg
    26761 aaccagtgag taaccattgg ccatcgtgat gttataagtg ccgccatcct gaacgctgac
    26821 ttctacacca acaatctggt ttaattcgct caccagttga tcgcgttgat ccagcagatt
    26881 gttaggtgac gcccctgccc ccacgcctgt caggcgggag atttgatcgt tcaggctggc
    26941 gatttgttta gcgtaattgt tgatctgatc aacgctggca cctatcgcga tattgacctg
    27001 tttgtcctgg tcgcgaagat actgatcggt ggttttaaac tgattcacca acccttctga
    27061 tttcccaatc agcgcctggc gcgctgccgg gtcttccgcg ttactcacca gcgtttgcag
    27121 gctggtgaag aaatcctgca tctgtgttgc cagcgaagag gtactggtgg agagcatatt
    27181 gtcgattttc gacatctgct catagcgggc agtcaggccg ctactttgcg tctgcgccgc
    27241 acgtaactgg ttggtaataa acgcatcata ctcacgctgc acaccagaaa cgtagacacc
    27301 attaccaacc cagccgccag cgcccaacgt gctattggcc tgcgccataa tagtggtttg
    27361 gcgggtatat ccggcaacgt tatagctgga gatattatta cttgccgtat ttaacgccgc
    27421 ctgggccgcg ttcagtccgc tcatggcgtt attgatcaag ctggacatgg aggttcctta
    27481 taagccttca attacagagt attatcggca gcgacccgcc ggacttgagt tattcagaac
    27541 agattatcaa tgttcatgct gtaggttttg ctcaccttgt cgcttatcga tttcatctgc
    27601 tgaatcatgt tggtgagttt acgggcatag tgaggatcgg tggcatagcc cgcgtcctgt
    27661 agggcctgcg ccccctgctc cgcactcgcg gcggtcgtca cggcggcgta gcgcgggtta
    27721 cgtgttaaca gcccaacgta atccgacaat gcttccagat acgagctgta gacccgaaac
    27781 ttcgctttta ctttcttcgc ttcgccattt tcatattcag tcgtggtgat ttcagtgact
    27841 ggccctttcc agttgccaga ggctttgaca ccaaacaggt tatagctcgg ctcgccgttt
    27901 tcgcggcgga tttgccgttg cccccagcca gattccagcg ccgcctgagc gaggatcaaa
    27961 tgatgtggca caccgctttg ctggcttgcc agttgggcgg gcagcgagag ttgcgcgagg
    28021 aatgctttac tgtcacctgg cagcgaatca tcgtagttac gtggcacggc cttttgcacc
    28081 agctgcgaaa gcgcctgatt ttgataacgc accacggttt cgagcgggaa tttcatcggt
    28141 gctgctggcg tggactcctc tggcaatggt tgttctggcg tcatctgctt aaccatcatc
    28201 tctgcaagcc ccagaccttt gcccgtcgtc atctgttggg caatctgctg gtcatacata
    28261 ctggtataca ggcgagtgtg ctcgctgctg aacaggccat cttttggtaa cgcgtcgcgc
    28321 atgcttttca acatcatctg cacgaacatc ccttccacct gacgggccac cggacggata
    28381 tttgccgccg gatcttcgct cgctttcgcc tttagttcgt tgagtgattg cgcatcccag
    28441 gccgcacttg ccagtagttt gctgtcgctg atcatcagat gatttccagt tttgcccgca
    28501 gacatcccgc actttgcatt gattgcaaaa tagacatcag atccatcggc gtagcgccca
    28561 gcgcattgag cgcgcgcacc acgttattga ggctggcgct ggaacgtacg ctttgcagcg
    28621 aaccgccgct ctggcgtaag tcgatctgcg tttgtggcgt taccacggtc tgtccgccac
    28681 caaacggtgt atctggttgg ctgacattgg cctgacgatt gactgttact gagagatttc
    28741 cctgcgctat cgcgcagctg tcgagagtca cttcgcgatt catcaccacc gaaccggtgc
    28801 gcgagttaat cactacttta gcgtcctgcg gggtgacatt aacctgcata ttctggatat
    28861 cggcaaggaa acggacctgg gaactgttgc cactcggtac gcgcacctga atagtccgcg
    28921 catccaacgc ggtggcgctg ccatatccac gcacgcggtt gatggtgtca gcgatttgct
    28981 gcgccatgct gaaatcttcg tcgttaagtt gcaaattaag ggtattcccg acgccaaact
    29041 ggctgggtaa ttcacgttca ataactgcac cattggtgat ccgtccaccg ttcagttggt
    29101 tcacctgaac actgctaccg ccagcggagg ctcctgcgcc gccaaccaga atattgccct
    29161 gcgccagcgc atacacctga ctgtcaacgc ccttaagcgg agtcatcaac aatgtgccgc
    29221 cacgcaggct tttggcattt cccatggaag aaaccaccac gtcaatggtt tgcccctgac
    29281 gtccaaacgg tggaagtgac gccgtcacca ttaccgcagc aacgtttttt agctgcatat
    29341 tggtgcccgt cggaacggta attcccagct gtgagagcat gttattaagc gtttgtgtgg
    29401 taaacggcgt ctgggttgtc tggtcaccgg tgccatccag ccccaccacc agaccatagc
    29461 caatcagtga gttttgcctt accccctgaa cactggtgag atcgcgaata cgctcagcct
    29521 gagccgccgt cgtaaccagt agaagaatta atgcagagag aaatttaatc accacagcct
    29581 cacttacatt ggcgacaggt taaggaagaa acgctgcaac cagcccatat tttgcgcttc
    29641 gttaatgtag ccattgccta cgtattcaat gcgcgcatcc gccacctgag tagacggtac
    29701 ggtattgctg ccgctgatag tgcgtggatt aaccacgcca gagaagcgaa taaattcggt
    29761 accctgatta atggcaatct gtttttcacc caccacatgc aggttgccgt tgaccagtac
    29821 ctggtcaacc gtcaccgtca acgtgccgct aaaggtatta ctggcattgg ccccgccttt
    29881 tccgttgaac gtgttaccac cggaggcttc gacatcggca cgagcgttac caaacagccc
    29941 ctgcaaatag cgcggcacag tatcaaagcc aaaattagtt ttaccgtcac ggctggcatt
    30001 cgcagaggag cttttgctgg cgctaacgtt ctcctgcaac acgatggtca gcgtatcgcc
    30061 aatattgcgt ggtcgacgat cttcaaacag cggttgatag ccatagttaa tcggctgagc
    30121 agactggaaa atagaaccgt tggcgacggg cgtcggaccg ggaaccggtt gtgcactggt
    30181 cgccccctgc accagcggcg tggagggtat ccaggcgcag ccggttagtg aaagcaccaa
    30241 caagctggaa atggcataag tatgcgcagc gtttttttgc atggctatca tcttcaaaaa
    30301 tcaataaacc ggtgaacctg ccaccggtta tgccttagag ttgcgtcagt ttttgcagca
    30361 tctgatcggt ggtggacacc gctttactgt tgatttcgta agcgcgttgc acctgaatca
    30421 tattgaccag ttcttccgcc acgttgacgt tagacgtttc aacataccct tgatacagca
    30481 gtcccgcgcc gttcaggccc ggcgtgcttt cgttcggtgc accggaggat tgcgtttcgg
    30541 tgtagaggtt ttcgccaatg ctctccagcc cggtgtcatt cataaaggtg gtgagattga
    30601 gctgcccaac ctgaaccgga gctgcctggc cttgttgggt tacgctgacc acgccatcac
    30661 gaccgatggt gatacttaac gcattcgccg gaatggtgat cgctggctgc acctgaaaac
    30721 caccagccgt caccagctgc ccgttctgat ccacctggaa agagccgtca cgggtatagg
    30781 ctgatgagcc atctggcaac atcacctgga aaaagccctg ccctttaatc gcgacatctt
    30841 tgctgttgtt ggtctgcgac aggttgcctt ggctgtgtaa gcgttcagtg gcgaccgggc
    30901 gtacccccgt gccgatttgt aatccggagg gtaaggtggt ttgttcggaa gactgtgccc
    30961 ccggctggcg aatggtttga taaagcagat cttcaaacac cgcgcgctga cgcttaaaac
    31021 cgttagtact gacgtttgcc aggttgttgg caatgacgtc catattggtt tgctgggcgt
    31081 caaggcccgt tttggcgatc cataatgaac tgatcataaa atgtcctgta gtggattgct
    31141 gacaagcaga gcagaagcac gcggcggtgc gaatttcccc tcaccctaac cctctcccca
    31201 aaggggcgag ggaacagtcc gctcgctttt attgcaatgt catcaatcgt tcaattaact
    31261 catcgacagc agttggttgg cacggcctgc gttatcatcg acgctgctga tcaccttcat
    31321 ctgcatttca aaacgccgcg cgctggcaat catgtcgctc attgccgcaa cggcattgac
    31381 gttactgcct tccagaaccc ccgacatcac acgcaaggtt ggatctgcct gcagtaccgg
    31441 tccacgcgtg gcctgggttt ctgcgcttaa acgaaaaatg ccgtcgtcac cgcgctgcac
    31501 ttcgctgccc gtggctttca ccagtttcag acgccccact ggcgcaaccg tatttgccgg
    31561 atcgcccgga ttcagcgccg agattgttcc atcggcagct atcgtgattt ccgccccttc
    31621 cggcacagca attggccccg cctcgcctat caccggatgc ccctgaattg tcagttgccc
    31681 ggtgggatca acctgaatgc tgccattacg cgtataccct tcgctgccgt cagcggtctg
    31741 cacggccaac cagccatcct gctgcaacgc aacgtccagc gggcgcgagg tgtaatccat
    31801 cttgccgggc gttatgtctg cacccggtgt tgacgccgtt accagcgtgc gcgtgggcag
    31861 tgaaagccct tccaccggca ctgcgcgcaa ggcgtttaac tgcgcgcgaa aaccgggcgt
    31921 agaggcattg gccagattac tggcggttac cgcctgttga ttcagcgtct ggctggctgc
    31981 tcccatcgcg gtataaattg cgtgatccat tgagctatcc cgtcagcgat tagcgtaagt
    32041 taaccagcgt gttgaggatc tggtcctggg ttttgatggt ctgggcgtta gactgatagt
    32101 tacgctgggc aacgatcata ttgaccagtt ctttactgag atcgacgttg gacgcttcca
    32161 acgcaccgtt ggtcagggtg ccaaagttgc ccgtcccggc tgtccccaac agcgccacgc
    32221 cagaagattg cgtcgcagac cagacgttgt cgccttcgga tgccagacct tcgttgttgg
    32281 caaagttcgc cagtacaatc tgccccagca gttgggtttg ttcgttggaa tagttgccga
    32341 caaccgtacc gtcatcattg atttgataac tcaccagatc gcccggtttg tagccattct
    32401 gggtggttgc cacaatgttg ttagcgccgg tattttgctg catggagttg aggaagctca
    32461 ggctaaacgt ggcgggttct gcgccgttaa ttgcgccggt ggtaatattc tctgttggat
    32521 ttgaggtcag aacgccattg gcattaaaca ccagcttcat tgcaggctcg gctgtacctg
    32581 ttggatcact gctatcctgg gtgtagacct gccagttatt atccccggtc ttcacaaagt
    32641 agacgctcat gtcatgagca ttaccctgac tgtcgaaaac agtcaccgaa ccttttttgt
    32701 tatagctatc cgcattgctg gcatcaaatg cgttaacaga gggaagcgga tcgctggaat
    32761 tcaggttgat ctgcatcgac gccgtggtgg tagttttcgc tgccatcagg gtattcggga
    32821 tcgaaatgtt agtcggattc gccccttgct gaatagtcgg cggcgtaccg gttgccgggt
    32881 aacccgtcag ctgtaaacct tgcatattca ccaggttacg attttcatcc agcttaaatt
    32941 gtccgttacg gctgtagaac accgaaccgt tgctatctac cagacggaaa aaaccgttct
    33001 ggctgatagc aacgtccaga ccacgcccgg tgttggtggt cgtgccatcg gtaaagtcct
    33061 gagtgatacc ggcaactttt acccccagtc ccactttcga accggcaaac atatcggcaa
    33121 aagaggccgt gcctgattta aagccgtagg tggcggagtt ggcgatattg ttgccaataa
    33181 catcgaggtt ggtggcggca gcgtttaatc cgctaaccgc ttgagaaaag gccatgactg
    33241 actcctgaaa tgtgaaggct taaattatct gccgtacttc gtcgagggtg gtggtgccgt
    33301 aagtgccgag atccagcgta ttaccgttgt tgccgcggat cacaccctgc accagagcaa
    33361 actgcagcgg ctgggcaacc agttgtgtac caccgttact ggcgctaatc gctacgttgt
    33421 aagaaccgtt cggcgcagtg ctgccgtcag tcaacgtacc gtcccaggtg aaactgtgaa
    33481 ctccggcggt cagttcacca atatcaatgg tgcgcacaac cgcgccattt ttatcggtga
    33541 tggtggccgt aactttgtct gccgcctgtt gcagctcaac accaaacggc gtggtcgtgg
    33601 tcacagcccc ttcttcactg ccggttcccg caagaacagt ggtgccgggg atcatcacgc
    33661 cgtgaccgat caggttactg gcctgtaacg actggctgtt atcaatctgt ccggaaatag
    33721 atccgagcgt ggtattgagt ttttcaatcc cgctgaccgt gctgatttgt gccaattgcg
    33781 acgtcagctc gttgttttcc attggattgg tcgggtcctg gtttttcagc tgcgccacca
    33841 acaaagtcag aaaactgctt tgtaaatctg cggcgttgct gcccgtgagc gaactactgc
    33901 tggtggtact gacgccggta tttgtcggat cggtggtggt taccgcaatg gacatagctt
    33961 tctcctttat tgaccgagcg taagggtttt cagcatcatg cttttcaccg tgttgagcac
    34021 ttcaacattg gcctgatagc tgcgtgacgc cgacatggtg ttaaccatct ctccgacaac
    34081 atcaacgttc ggcattttta cgtagccctt tgcatccgcc agcggattac ccggttcata
    34141 aaccagtttg tccggggcct gactttctat aacatcggca acctttacgc cgcctgtcgc
    34201 agcacctggt gcagcgttaa cctggaatac cacctgtttt gcccgatatg gctgtccatc
    34261 aggaccggtc acgctatcag cattcgccag attactggcc gccacgttca ggcgctggga
    34321 ctgggcagtt aacgccgacc cggcgatatc aaaaatattc agcagtgcca tccgttaatt
    34381 tccgctctgt aaaacgttca tcatgccttt gatttgcccg ctcaacgcgc taaggctcat
    34441 ctggtattgc aggctgttat cggcaaactg ggtgcgttcg cgatccatat cgacggtatt
    34501 accgtcaagc gaaggctggt ccggaatacg gtattgcagt tctgcggtag gaggcgtcag
    34561 cgcctgcgcc ggaatgtgtt gcgttgaggt catcgtcaga gcaaccacac tggttgcatt
    34621 ccgtccacgt tgcatgactt ttttaagttc actggcaaaa tcgatatcgc gcgcctgata
    34681 accaggggta tcggcattgg cgatgtttgc tgccagcact tcctgacgct gggcgcgcag
    34741 attgagcgcc tcttgttgaa aacgtaaggc ggcgtcgagc ttatcgagca tatctcctcc
    34801 gcaggtatca aaattctgcc atcagcttaa atgcctttac gcgcgcgtta tcggcggaat
    34861 aaacgcaaaa tgggtcgcta tttatgccgt tgatggtcat tgtggacagg tacaattcac
    34921 gttgtagaaa tggctggggg cgaaaatgct ggcaataaaa cgtagcgtgg cgatcatcgc
    34981 gatactgttc agtccgttaa gtgcggcgag caatctcaca tcgcaattgc aaaccttttt
    35041 tagctcccag ctcgcaggga taagtgatga ggttcgtgtt tctattcgta cagcgcccaa
    35101 tctactaccg ccgtgcgagc agccattgct ttcgatgagc aataattccc gcttgtgggg
    35161 caatgtgaat gtgttggcac gctgcggtaa cgacaaacga tatttacagg ttaatgtgca
    35221 ggccacagga aattatgtgg ttgccgcgat gcccattgtg cgaggaggaa agctgggagc
    35281 tggcaatgtc aaactgaaac gcggacggct ggataccttg ccaccgcgta cggtgctgga
    35341 tatcaatcaa cttgttgatg ccgttagcct gcgcgatcta tcacccgatc aacctatcca
    35401 gttaacccag tttcgccagg catggcgggt aaaagcggga caacgcgtca acgtgatcgc
    35461 cagcggtgat gggtttagcg ccaacgcaga aggtcaggcg ctaaacaatg cagccgtcgc
    35521 acagaatgcg cgggtgcgca tggtatctgg acaggtagtc agcggcgttg ttgatgcaga
    35581 tgggaatatt cttataaacc tgtaagctgt taaagattac ccgtcccttg ccgataaata
    35641 agcaacacat gataaaagcg ccctcaatga ggaataaacc atgagtattg atcgcacttc
    35701 gcctctgaag cccgtaagca ccgttcaacc gcgcgaaacc actgacgcgc cggtaacgaa
    35761 cacccgagcg gcaaaaacaa cagcctccac cagcaccagt gtgacgttaa gcgacgcgca
    35821 agcaaaactg atgcaacccg gcagcagtga tatcaatctt gaacgtgtcg aagcgttaaa
    35881 actggcgatt cgtaacggtg aactaaaaat ggacaccggc aaaattgccg atgcgctgat
    35941 caacgaagcg cagcaagact tgcagagtaa ttaatcgtat gacgcgtctt gcagagatcc
    36001 tcgaccagat gtccgctgtg cttaacgatc tcaaaacggt aatggatcaa gagcagcaac
    36061 atctctctat ggggcagatc aacggcagcc agttgcaatg gattacagaa caaaaaagct
    36121 cactgctggc aacgttggat tacctcgaac agttacgcag gaaagaaccc aatacagcaa
    36181 atagcgttga tattagtcaa cgctggcagg aaattgctgt gaaaacgcag caactacgcc
    36241 aaatgaatca acataacggc tggttactgg aaggacagat tgagcgcaat caacaggcgc
    36301 tggaaatgtt gaaaccgcat caggaaccga cgctatatgg ggcgaacggt cagacctcaa
    36361 caacccatcg cggcagtaaa aagatttcga tctgaagatt atctccggcc tgcactgcag
    36421 gccggaatgc attgttacac cgtccggcgg gcaaattctt taactttgaa gcccagtacc
    36481 gccagtgcag cgaagtacgc ggcaatcccc gccagcacga ccgccattaa acgcagcaaa
    36541 cgccagggca tggtacccaa tgaccactcc ggcatgatat gtaacatacc taaaagcacg
    36601 ccagacatca ccagtaccgc caccaccaga cgcaacagaa acgccatcca gccaggttgc
    36661 ggggtaaaga ttttctgctt acgcaactgc cagtaaagca gcgaagcatt cagacacgcc
    36721 gccagaccaa tagaaagtga cagcccggca tgtttcaacg gaccgataaa cgccaggttc
    36781 atcaattgcg ttaaaatcag cgtaacgatg gcaattttca ctggcgtttt aatgtcctgg
    36841 cgggaataaa agccaggagc caacactttc actacaatca ggccaatcaa acccaccgag
    36901 taggcaatta acgcccgctg ggtcatcagc gcatcaaagg cggtaaattt accgtactgg
    36961 aacagcgaaa cggttaacgg accggaaaga atgcccaacg caaccgcact cggcaacgcc
    37021 aacaggaaac aaagacgcag tccccagtcc atcaaacggt tgtattcatc atgattgcca
    37081 ctggcaaaac ttttcgacag cgacggtagc aaaatggtgc caagcgccac gcccagcaca
    37141 ccggacggaa actccattaa gcggtcggcg taatacatcc atgacaccga accggaagca
    37201 agaaacgagg caaaaatggt gttgatgatt aaggagatct ggctcacaga gacgccaagg
    37261 attgccggtc ccatctgttt caccacacgc attgctccgg catcgtggaa gttaatgcgt
    37321 ggcaaaacca gcatgccgat cttcttcaga tgcggtagct gatacaccag ctgcaggacg
    37381 ccgccgaccg tcacagccca cgccagcgcc agcaccggtg ggttaaagta cggtgcggca
    37441 aacagcgcga aaccaatcat gctgatgtta agcagtgttg gagcaaacgc cggaatcgag
    37501 aagcggttcc acgtattcag aatcgctccc accagcgacg ccagggagat cagcaagata
    37561 tagggaaagg taatctttag tagctggctg gtcagggcaa atttgtcagc tgtgtcagcg
    37621 aagcctggcg cggtcaccat gatcacccac ggtgcggcga gcatgccagc gaccgtcaca
    37681 accgccagcg caagtgtcag caggccagaa acataagaga caaagacccg cgtggcgtct
    37741 tcaccctgct tacttttata ttccgccaga atcggtacaa atgcctggga aaatgcccct
    37801 tcggcaaaga tacggcgtaa caagttagga agtttaaaag cgacgaaaaa ggcgtcggtt
    37861 gccatccctg cgccaaagat tctggcgaca attgcgtctc gtgcgaagcc aagcacacgc
    37921 gaaaacatgg tcatcgagct gacggcggcc agcgatttta ataaattcat cggtgttcta
    37981 atccagaccc acgggcaaaa cgcctgcaag gcaggcgctg aaagaggcga atagtctacc
    38041 tggattatgg tgaattgcta ccgccagatg ttacagggtt tattcactca tcgcatcgcg
    38101 ccagatcttg tcaacgatac gttgcgccag cactgcctgt tcgccggcgg tttgcggaac
    38161 tgtctgattt tgcacgcatt caatgaagtg ccgcgcacag ccgacaaatc cacgttgctc
    38221 aagcgtgctc tgccaaccag gaatcggttt attcactaca ccctgcccac gctcctcacg
    38281 ccattcgcgc atttccgtaa tgtcgatgag cgcaccgtca gtcacggcct gcacggtttc
    38341 acgctgactt ccggcacggc gatgcataca agtggtgatc tgcaaaggac cggccgaaaa
    38401 atggtgctcg gcaaacagca tttcgccagc gtcgttagtc agtagcgtac cgccatccag
    38461 agaggctttg ccgcccgaca accacagcgc ggtatccacc acatgcagat aatcatccag
    38521 caacgtgaaa taaagatcgt gtggcccgac gctattgcta cgatgtttat ccattcttag
    38581 cgaggctgcg gtggcgagtt gcgtttttaa ctcacagtag agtggtgcga aacgacggtt
    38641 aaaaccgacc atcaacgtca gttttttccg cgccgccagt tccaccagcc gttcagcatc
    38701 gcgcagattt tctgccagcg gtttatcgac acagacatgt acccccgcat tgagtaacgt
    38761 actgaccacg tcaaagtgac tggcggtgct ggaatgtaca aaaaccgcat cgcaactggc
    38821 ggcaaggctg gataacgaat cggcataagg aatgcgccag ctttcacaaa ttggcagggc
    38881 tttcgcgcgc gtaggcgacc aggctccttg taacgtccag tcagacgctg ccgccagcac
    38941 cggtaaccac gctttttgcg caatgccgcc taatcccact acgccgatac gtaatttttt
    39001 cactttaatc tcccaggtgg gccagcaacg aatcaagacg ctgtttcagt tctgccactt
    39061 cgatttccag ggcttcgacg cgggcctgta aatcaccgtc aaccgcgttc gacatatccg
    39121 ttaccgccgg ctgatcctct acctcaccac tgaaaagatg catgtagcgg ctttcgcgtt
    39181 tacccggttc gcgggccaga cgcaccacaa aaggaccatc ttcgcgattc gccagttgtt
    39241 ccagcgtcaa ctccacttcc gccatatcgc tgaattcata cattcgcgcg gcgcggctgc
    39301 gcagttcacc cggcgtctgg gcaccacgca ataacaacgt ggtgattaac gccacttctg
    39361 cggcgctcag tttcagatcg ccaaattctg aattacaaaa acgttgctca tatttggtga
    39421 cccgattacc aaaaccgctc actgtgcgta gataatgacg tttgaccaga ttatccagct
    39481 gttcctgcac ttcagattca ctcagattca tgaccggttc acggttcgtt ttctgattac
    39541 aggccgtgac tacaccattg actgagagcg ggtattgctc cggcgtggtc acctgctttt
    39601 ccagcaagca accaatcaca cgagcttcca gggcagtaag ttgatatttc atgagattct
    39661 ccttagcggc cgggcgtcca gtctggggta gttaatgccg tcagtacgtg atcgcgccat
    39721 tgtccatcaa tcaacagata gtctttcgca tagccttctt tttcaaaacc cagtcgcgcc
    39781 agtaaatcac cgctgcgttt attgtgcggc atataattag ccataatgcg atgaatatgt
    39841 tgggtgcgct gcatataacg aatggctgcg gtcagggctt caaacatgag tcctttgccc
    39901 tgccattttt gcccaatcga ataaccgaga tagcaggcat gaaaagagcc gcgaacaaca
    39961 ttggaaaaat tggcaacgcc aataatctct ttttcgtccg ggtcgaataa gccaaagtag
    40021 aaagctgaac cttgtttatg aaattcgtta atcatcccca gcctggcctg ccagcctgat
    40081 ggataacagt ggctttcgtc gcgcactggc tcccagggct tgaggaaatg gcgattctct
    40141 gcgtaataat ccgccagacg ccaggcatca cgatcatgca ccagacgcac gaccagtcgg
    40201 tctgtggtta agcgcacttt tggcacgtta ctgcgatagc caaacattct atacctactc
    40261 cttcccgtaa cgtctttaat acgctgtaag tattactata cctgcgcaac cagaccctgt
    40321 gaaaagtcca aaatgccatt ttctgcatta ttcacatatt tgataaaaac tctctatcaa
    40381 agcgacgttt tgataaaaaa atattgtccc cgaagtgatg ggaaaagtct tagcggcaat
    40441 gcagaataga aagtgctcat cttttatttc ccgggagggg aaatgtcccg cgtgtcgcag
    40501 gcgaggaacc tgggtaaata tttcctgctc atcgataata tgctggtcgt gctggggttc
    40561 tttgttgtct tcccgctgat ctctatccgc ttcgttgatc aaatgggctg ggccgccgtc
    40621 atggtcggta ttgccctcgg cctacgccag tttattcagc aaggtctggg tattttcggc
    40681 ggcgcaattg ccgaccgctt tggtgccaaa ccgatgattg ttaccggtat gctgatgcgt
    40741 gccgccggat tcgccacaat gggtatcgcc cacgaaccgt ggctattgtg gttttcatgc
    40801 ctgctctcgg gactcggtgg cacgttgttt gatccgccgc gttcggcgct ggtggtgaaa
    40861 ctaatccgtc cacaacagcg tggtcgtttt ttctcgctgt tgatgatgca ggacagtgcc
    40921 ggtgcggtca ttggcgcatt gttggggagc tggctgttgc aatacgactt tcgcctggtc
    40981 tgcgccacag gggccgtttt gtttgtgctg tgtgcagcgt tcaatgcgtg gttgttaccg
    41041 gcatggaaac tctccaccgt acgcacgccc gttcgcgaag gcatgacccg cgtgatgcgc
    41101 gacaagcgtt ttgtcaccta tgtcctgacg ctggcgggtt actacatgct ggctgtacaa
    41161 gtgatgctga tgctgccaat tatggtcaac gatgtggctg gcgcgccctc tgccgttaaa
    41221 tggatgtatg ccattgaagc gtgtctgtcg ttaacgttgc tctaccctat cgcccgctgg
    41281 agtgaaaagc attttcgtct ggaacaccgg ttgatggctg ggctgttgat aatgtcatta
    41341 agcatgatgc cggtgggcat ggtcagcggc ctgcaacaac ttttcaccct gatttgtctg
    41401 ttttatatcg ggtcgatcat tgccgagcct gcgcgtgaaa ccttaagtgc ttcgctggcg
    41461 gacgcaagag ctcgcggcag ctatatgggg tttagccgtc tgggtctggc gattggcggc
    41521 gctattggtt atatcggtgg cggctggctg tttgacctgg gcaaatcggt gcaccagcca
    41581 gagcttccgt ggatgatgct gggcattatt ggcatcttca ctttccttgc gctgggttgg
    41641 cagtttagcc agaaacgcgc cgcgcgtcgt ttgcttgaac gcgacgcctg atttgatact
    41701 cccccctgac tcctccatac tgaaagtgaa ctgacagcca aatggaggag tcacgtgaag
    41761 ctatacattt acgatcactg cccttactgc ctcaaagccc gcatgatttt cggcctgaaa
    41821 aatatccccg tcgaattaca tgttctgctc aacgacgacg cagaaacacc cacccggatg
    41881 gtcggtcaaa aacaggttcc cattctgcaa aaagatgaca gccgctatat gccagaaagc
    41941 atggatatcg ttcactatgt cgataaactc gacggcaaac cgttactgac cggcaaacgt
    42001 tcccctgcca ttgaagagtg gctgcgcaag gtcaatggct acgccaacaa actgctgttg
    42061 ccgcgttttg ccaaatcggc atttgatgag ttttctactc ccgccgcgcg caaatatttc
    42121 gtcgacaaga aagaggccag cgcgggtaat tttgccgacc tgctggccca ctctgacggt
    42181 ctgattaaga atatcagcga tgatttacgt gcgctggaca aactgatcgt caaaccgaac
    42241 gccgtgaatg gcgaactttc ggaagatgat attcagctat tcccgctact gcgtaatctg
    42301 acgctggtag ccggaattaa ctggccaagc cgcgttgctg attaccgcga taatatggcg
    42361 aaacagacac aaatcaattt gttatcatca atggcgattt aatcctgact cgccgcggca
    42421 gcctgtattc gcgggttgcc gccgttttca gttttttctg ctggcgagtt tatcgctggt
    42481 gacacatgct gcgttgtgtc aggatattga cgcagatttc atgtcgagga ctaccatgaa
    42541 caagttttta tttgccgctg cattgatcgt cagtggcctg ctcgttggct gtaatcaact
    42601 cacccaatac accatcaccg aacaagaaat taaccagtcg cttgcgaaac ataataattt
    42661 ctcaaaagat attggtttac ccggtgtggc tgacgcccat attgttctga caaacctgac
    42721 cagccaaatt ggtcgcgaag agccgaataa ggttgcccta accggagacg ccaatctgga
    42781 catgaactcc ctgttcggta gccagaaagc gaccatgaaa ctgaagctga aagcgctgcc
    42841 cgtgtttgat aaagaaaaag gcgcgatctt cctgaaagag atggaggtgg tcgatgcgac
    42901 ggtacaaccg gaaaaaatgc aaacggtgat gcaaacgttg cttccctatc tgaaccaggc
    42961 attacgcaat tactttaacc agcaacctgc ttacgtcctg cgcgaagatg gcagccaggg
    43021 cgaagcaatg gcgaaaaaac tggcgaaagg cattgaagtg aagccaggcg aaattgtcat
    43081 tccatttact gattaatcac gagggcgcat tcgcgccctt tatttttcgt gcaaaggaaa
    43141 acgtttccgc ttatcctttg tgtccggcaa aaacatccct tcagccggag catagagatt
    43201 aatgactgca ccatcccagg tattaaagat ccgccgccca gacgactggc accttcacct
    43261 ccgcgatggc gacatgttaa aaactgtcgt gccgtatacc agcgaaattt atggacgggc
    43321 tatcgtaatg cccaatctgg ctccgcccgt gaccaccgtt gaggctgccg tggcgtatcg
    43381 ccaacgtatt cttcacgccg tacctgccgg gcacgatttc accccattga tgacctgtta
    43441 tttaacagat tcgctggatc ctaatgagct ggagcgcgga tttaacgaag gcgtgttcac
    43501 cgctgcaaaa ctttacccgg caaacgcaac cactaactcc agccacggcg tgacgtcagt
    43561 tgacgcaatc atgccggtat tagagcgcat ggaaaaaatc ggtatgccgc tactggtgca
    43621 tggtgaagtg acacatgcag atatcgacat ttttgatcgt gaagcgcgct ttatagaaag
    43681 cgtgatggaa cctctgcgcc agcgcctgac cgcgctgaaa gtcgtttttg agcacatcac
    43741 caccaaagac gcagccgact atgtccgtga cggaaatgaa cggctggctg ctaccatcac
    43801 tccgcaacat ctgatgttta accgcaacca tatgctggtt ggtggcgtgc gtccgcacct
    43861 gtattgtcta cccatcctca aacgcaatat tcaccaacag gcattgcgtg aactagtcgc
    43921 cagcggtttt aatcgagtat tcctcggtac ggattctgcg ccacatgcac gtcatcgcaa
    43981 agagagcagc tgcggctgcg cgggctgctt caacgcccca accgcgctgg gaagttacgc
    44041 taccgtcttt gaagagatga atgctctgca gcactttgaa gcattctgtt ctgtaaacgg
    44101 cccgcagttc tatgggttac cggtcaacga cacattcatc gaactggtac gtgaagagca
    44161 tcaggttgct gaaagcatcg cactgactga tgacacgctg gtgccattcc tcgccgggga
    44221 aacggtacgc tggtctgtta aacaataaaa atcgtagctt cctgttgtca ttaggttatt
    44281 ttacctgtat aaataaccag tatattcaac agggggctat tatgcgaatt gaagtcacca
    44341 tagcgaaaac ttctccattg ccagctgggg ctattgacgc cctggctggc gaactttccc
    44401 gccgtattca gtatgcgttt cctgataatg aaggccacgt atcagtacgt tatgccgcag
    44461 cgaataattt atcggttatt ggcgcgacaa aagaagataa acagcgcatt agcgaaattc
    44521 tccaggaaac gtgggaaagc gccgatgact ggtttgtcag cgaataatat gcagtgattt
    44581 ttgtttttgc cgggtcgccc cggctttttt attttacttg cgaattatga gtacaaaatt
    44641 tctacacttc ctcaaaataa ttgcacaata tgctaaaaaa atatgaatga catgctgttt
    44701 tattgttcat ataaatctta aaagcagaaa tttatctgtt ccccccttta aaaattcggt
    44761 gattaccctt atttattggt atactgaagt tgcttcagat aaaaacgcat tgaacctcga
    44821 ataacgttgt ctagtaacac gaattagggg gccatgatgg aaaaaaataa tgaagtcatt
    44881 cagactcatc cgctcgtagg gtgggacatc agcaccgttg atagctatga tgcgctgatg
    44941 ttgcgtttgc actaccagac cccaaataag tccgagcagg aagggactga agttggtcag
    45001 acgctctggt taaccactga tgttgccaga caatttattt cgatattaga agcaggaatc
    45061 gccaaaattg aatccggtga tttcccggta aacgagtatc ggcgtcatta acatgacatg
    45121 accatccacc caaaaggcac ctcaccggtg cctttaccat ttctgggttg tatatctgca
    45181 ttcagttaat tactctgctt aaaccatgag agcagagaga aaaaaatgaa atacgatctc
    45241 atcattattg gcagcggttc cgtaggcgct gccgccgggt attatgcaac ccgcgccggt
    45301 ttaaacgtgc ttatgaccga cgcccatatg ccaccacatc aacacggcag ccaccacggc
    45361 gatacgcgat taattcgaca tgcttatggt gaaggcgaaa agtatgtccc gctggtcctc
    45421 cgcgcacaaa cgctgtggga tgacctctcc cgccacaacg aagatgatcc catttttgta
    45481 cgctctggtg tcattaacct tggcccggct gactccgcat ttctcgccaa cgtcgcccac
    45541 agcgccgaac agtggcaact caacgttgaa aagctcgatg cgcaagggat tatggcccgc
    45601 tggccagaaa tacgcgtccc ggacaactac atcggcttat ttgagactga ttccggtttt
    45661 ttgcgcagcg aactggcgat taaaacctgg atccaactgg cgaaggaagc gggctgtgcg
    45721 caactgttca actgcccggt caccgcaatt cgtcatgacg atgatggcgt aactattgaa
    45781 acggctgacg gtgagtatca ggcgaaaaaa gcgattgtct gcgcgggaac atgggtaaaa
    45841 gacctgctcc cggagctgcc tgtccagccc gtacgtaaag tatttgcctg gtatcaggcc
    45901 gatggccgct atagcgtgaa gaataaattc ccggcgttta ccggtgaact gcccaatggc
    45961 gatcaatatt atggttttcc ggcagaaaac gacgcgttga agattggcaa acataacgga
    46021 ggccaggtta tccattcagc ggatgaacgt gttccgtttg cggaagtggt cagcgatggt
    46081 tcggaagcct tcccgttctt gcgcaatgta ttgccgggta tcggttgctg cctgtacggc
    46141 gctgcctgca cctatgataa ttcgcctgac gaagatttta ttatcgatac cctacccggc
    46201 cacgataata cactgctcat taccggcctg agtgggcacg gttttaaatt tgcgtcagtt
    46261 ttaggggaaa tagctgccga ttttgcgcaa gacaaaaaaa gcgattttga tttgacgcca
    46321 ttcagccttt cccgcttcca ataatcatca ttacggcctc cagaatgagg ccgtttttac
    46381 aggagtattt aatgcgtcgg ctgttgcact atctcatcaa taatattcgc gagcatctga
    46441 tgctatatct ttttctctgg ggattgttgg caattatgga tctgatctat gtgttttatt
    46501 tttagttttc cgcactgaca
//
LOCUS       DBFIFM010000010        46310 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.010, whole genome shotgun sequence.
ACCESSION   DBFIFM010000010 DBFIFM010000000
VERSION     DBFIFM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.010"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..704)
                     /gene="lpxT"
                     /locus_tag="N7C74_000537"
     CDS             complement(<1..704)
                     /gene="lpxT"
                     /locus_tag="N7C74_000537"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HGU2430477.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHF"
     gene            complement(743..1729)
                     /gene="yeiR"
                     /locus_tag="N7C74_000538"
     CDS             complement(743..1729)
                     /gene="yeiR"
                     /locus_tag="N7C74_000538"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HGU2430478.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVAGHLLDLPRHNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(1847..3313)
                     /gene="yeiQ"
                     /locus_tag="N7C74_000539"
     CDS             complement(1847..3313)
                     /gene="yeiQ"
                     /locus_tag="N7C74_000539"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HGU2430479.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRDLVAGSISEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQARQQIAQFGAHQALLNTLKI"
     gene            complement(3536..4108)
                     /gene="yeiP"
                     /locus_tag="N7C74_000540"
     CDS             complement(3536..4108)
                     /gene="yeiP"
                     /locus_tag="N7C74_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HGU2430480.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            4263..4517
                     /gene="yeiW"
                     /locus_tag="N7C74_000541"
     CDS             4263..4517
                     /gene="yeiW"
                     /locus_tag="N7C74_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HGU2430481.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(4514..5695)
                     /gene="setB"
                     /locus_tag="N7C74_000542"
     CDS             complement(4514..5695)
                     /gene="setB"
                     /locus_tag="N7C74_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HGU2430482.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGVLVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            6063..7193
                     /gene="fruB"
                     /locus_tag="N7C74_000543"
     CDS             6063..7193
                     /gene="fruB"
                     /locus_tag="N7C74_000543"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HGU2430483.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            7193..8131
                     /gene="fruK"
                     /locus_tag="N7C74_000544"
     CDS             7193..8131
                     /gene="fruK"
                     /locus_tag="N7C74_000544"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HGU2430484.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            8148..9839
                     /gene="fruA"
                     /locus_tag="N7C74_000545"
     CDS             8148..9839
                     /gene="fruA"
                     /locus_tag="N7C74_000545"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HGU2430485.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKNESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            10263..11204
                     /gene="psuK"
                     /locus_tag="N7C74_000546"
     CDS             10263..11204
                     /gene="psuK"
                     /locus_tag="N7C74_000546"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HGU2430486.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRERLSQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            11192..12130
                     /gene="psuG"
                     /locus_tag="N7C74_000547"
     CDS             11192..12130
                     /gene="psuG"
                     /locus_tag="N7C74_000547"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HGU2430487.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAVIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            12224..13474
                     /gene="psuT"
                     /locus_tag="N7C74_000548"
     CDS             12224..13474
                     /gene="psuT"
                     /locus_tag="N7C74_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HGU2430488.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVAAALLLQIALGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATELSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(13545..14243)
                     /gene="yeiL"
                     /locus_tag="N7C74_000549"
     CDS             complement(13545..14243)
                     /gene="yeiL"
                     /locus_tag="N7C74_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HGU2430489.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            14373..15314
                     /gene="rihB"
                     /locus_tag="N7C74_000550"
     CDS             14373..15314
                     /gene="rihB"
                     /locus_tag="N7C74_000550"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HGU2430490.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGDTGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDSDWFWGLVEECVRGYIKTH"
     gene            15414..16664
                     /gene="nupX"
                     /locus_tag="N7C74_000551"
     CDS             15414..16664
                     /gene="nupX"
                     /locus_tag="N7C74_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HGU2430491.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESIVGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(16720..17808)
                     /gene="yeiI"
                     /locus_tag="N7C74_000552"
     CDS             complement(16720..17808)
                     /gene="yeiI"
                     /locus_tag="N7C74_000552"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGU2430492.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQSASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDET
                     VLAINDTHLLEQLSPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLAGEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(17811..18668)
                     /gene="nfo"
                     /locus_tag="N7C74_000553"
     CDS             complement(17811..18668)
                     /gene="nfo"
                     /locus_tag="N7C74_000553"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HGU2430493.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARTVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(18742..19791)
                     /gene="yeiH"
                     /locus_tag="N7C74_000554"
     CDS             complement(18742..19791)
                     /gene="yeiH"
                     /locus_tag="N7C74_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HGU2430494.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            19890..20771
                     /gene="yieE"
                     /locus_tag="N7C74_000555"
     CDS             19890..20771
                     /gene="yieE"
                     /locus_tag="N7C74_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HGU2430495.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            20976..22445
                     /gene="lysP"
                     /locus_tag="N7C74_000556"
     CDS             20976..22445
                     /gene="lysP"
                     /locus_tag="N7C74_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HGU2430496.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPGKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            22737..24716
                     /gene="cirA"
                     /locus_tag="N7C74_000557"
     CDS             22737..24716
                     /gene="cirA"
                     /locus_tag="N7C74_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HGU2430497.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFTA
                     NGVPVFSYYNVNKARIQGVETELKIPFNDEWKLSLNYTYNDGRDVSNGENKPLSDLPF
                     HTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVTKDVK
                     LRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(24748..25584)
                     /gene="yeiG"
                     /locus_tag="N7C74_000558"
     CDS             complement(24748..25584)
                     /gene="yeiG"
                     /locus_tag="N7C74_000558"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HGU2430498.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            25842..26510
                     /gene="folE"
                     /locus_tag="N7C74_000559"
     CDS             25842..26510
                     /gene="folE"
                     /locus_tag="N7C74_000559"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HGU2430499.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            26527..27684
                     /gene="yeiB"
                     /locus_tag="N7C74_000560"
     CDS             26527..27684
                     /gene="yeiB"
                     /locus_tag="N7C74_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HGU2430500.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMQFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            27826..28866
                     /gene="galS"
                     /locus_tag="N7C74_000561"
     CDS             27826..28866
                     /gene="galS"
                     /locus_tag="N7C74_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HGU2430501.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYTHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            29145..30143
                     /gene="mglB"
                     /locus_tag="N7C74_000562"
     CDS             29145..30143
                     /gene="mglB"
                     /locus_tag="N7C74_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HGU2430502.1"
                     /translation="MNKKVLTLSAVMASMLFGATAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            30204..31724
                     /gene="mglA"
                     /locus_tag="N7C74_000563"
     CDS             30204..31724
                     /gene="mglA"
                     /locus_tag="N7C74_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HGU2430503.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYKKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKRDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            31740..32750
                     /gene="mglC"
                     /locus_tag="N7C74_000564"
     CDS             31740..32750
                     /gene="mglC"
                     /locus_tag="N7C74_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HGU2430504.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQAPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(32993..34228)
                     /gene="preA"
                     /locus_tag="N7C74_000565"
     CDS             complement(32993..34228)
                     /gene="preA"
                     /locus_tag="N7C74_000565"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HGU2430505.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(34222..35460)
                     /gene="preT"
                     /locus_tag="N7C74_000566"
     CDS             complement(34222..35460)
                     /gene="preT"
                     /locus_tag="N7C74_000566"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HGU2430506.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLLRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDDFAKLEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(35654..35893)
                     /gene="yeiS"
                     /locus_tag="N7C74_000567"
     CDS             complement(35654..35893)
                     /gene="yeiS"
                     /locus_tag="N7C74_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HGU2430507.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(35896..36615)
                     /gene="sanA"
                     /locus_tag="N7C74_000568"
     CDS             complement(35896..36615)
                     /gene="sanA"
                     /locus_tag="N7C74_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HGU2430508.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(36765..37649)
                     /gene="cdd"
                     /locus_tag="N7C74_000569"
     CDS             complement(36765..37649)
                     /gene="cdd"
                     /locus_tag="N7C74_000569"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGU2430509.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LADAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            complement(37779..38474)
                     /gene="yohK"
                     /locus_tag="N7C74_000570"
     CDS             complement(37779..38474)
                     /gene="yohK"
                     /locus_tag="N7C74_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HGU2430510.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APILFPIILAVMG"
     gene            complement(38471..38869)
                     /gene="yohJ"
                     /locus_tag="N7C74_000571"
     CDS             complement(38471..38869)
                     /gene="yohJ"
                     /locus_tag="N7C74_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HGU2430511.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            complement(39000..39908)
                     /locus_tag="N7C74_000572"
     CDS             complement(39000..39908)
                     /locus_tag="N7C74_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU2430512.1"
                     /translation="MANWAQKLKLHHLEMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGITLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLTKMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYQVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            40035..41393
                     /locus_tag="N7C74_000573"
     CDS             40035..41393
                     /locus_tag="N7C74_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2430513.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAITRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASRVTAAFQIGGTLGALALGVLMDKFNPFRVLTLSYAIGAICIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAIGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTLLITVVRQSTSVPDSLPRAGVV
                     NE"
     gene            41405..42433
                     /gene="gtdA"
                     /locus_tag="N7C74_000574"
     CDS             41405..42433
                     /gene="gtdA"
                     /locus_tag="N7C74_000574"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HGU2430514.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGGLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTPMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNILGCGFAEDYPEEQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRLGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTD
                     STIYHVVEGSGQVIIGNETFSFSAKDIFVVPTWHGVSFQTTQDSVLFSFSDRPVQEAL
                     GLFREARY"
     gene            42448..43149
                     /locus_tag="N7C74_000575"
     CDS             42448..43149
                     /locus_tag="N7C74_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HGU2430515.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAVVPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEH
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKARDIGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETICYLSGFFELQPGDLIFTGTPEGVGPVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            43158..43802
                     /gene="maiA"
                     /locus_tag="N7C74_000576"
     CDS             43158..43802
                     /gene="maiA"
                     /locus_tag="N7C74_000576"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HGU2430516.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGINYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLERHYPQVPLVPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEEKKRWYAHWIQQGLSAVEQLLRQNQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            43817..45010
                     /locus_tag="N7C74_000577"
     CDS             43817..45010
                     /locus_tag="N7C74_000577"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HGU2430517.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARLGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEVARQRAVFTDHITMMDAVNAEEVVCIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWTGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDRADMPEDLRINAPVLWAGPHCHLVHYPL
                     RGGKQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIPPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTKRITLVGDSAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            45169..46119
                     /gene="dusC"
                     /locus_tag="N7C74_000578"
     CDS             45169..46119
                     /gene="dusC"
                     /locus_tag="N7C74_000578"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HGU2430518.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQECMAISGC
                     DSVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIGKLR"
BASE COUNT        11185 a        11476 c        12350 g        11299 t
ORIGIN      
        1 tggaaatgtt tgttttttcc cggtagtgat ttgtcaaaaa aggtgattaa tcgatcactt
       61 aatggcgtca gcaacaccca gggcaaaccg atcaatatca cggtcatcga accgacaatg
      121 atgtcagtaa accagtgtgc gccaatcatt actctgggaa acgcaaaaac cacaaaaata
      181 ataagggcga taagacctgc aactttgccg aaataacgcc acatgaatgc cgaaaaaata
      241 agcagcatca tgccgtgatc gccgggaaag ctatctcgtg aggcatcttt cgtggggaca
      301 gagagcagtt cgctgacgcg gttaatatcg gtaaaagtca atgttgggct ggcccgtttt
      361 acaggaatta atgcctgacc cagctggttt aataccactg cagttaatag catgaccaga
      421 ccaataatca cgatacgtcg tctgcctggg gcgttttctt tcagccagaa actcagcatc
      481 aacattccca ttgccagcag tgaacaaccg tcgaaagcgc gattgttggt caatgcaacc
      541 agccacaaaa aggccttact ttcgaccagt ttctgattaa agaaataaaa aatatccgca
      601 tcaatcggca accagaatcc atgattaacg ggaatatacc aggaaagaaa cagcgcgagg
      661 ccgacaatat tcaacaacac tatttgcggc aaatttttaa tcatattttc tctaattatc
      721 ttactgaaaa acgcaggcaa ccttacgcgg tagtcgctaa acgaagcttc aacagcgcgc
      781 tctgcaaggc attccagtca gcttcgctgc tggaaatcag ctcaatacgg ctgtccggtg
      841 gcgcaacgtt ttgcgtttca atgtgcaggt catcgccctg acggttgatt cgcaccagcc
      901 cttcgggaat acgcagcacg cctttgacgc gttccaccgg tgcaagtcgc gcccattcca
      961 gaatgccaat ggtgtcgaat accgtatcag cgtcgaatat ccagccgcag gcctgatgtc
     1021 cttgtccact gttcagactg cgacgccagc gttgatgctc tggcaggctt aacgctgcta
     1081 accctttttt cacgacatgc tgatgagaat gcgcggcgct ggcgggcaac tcggctaaat
     1141 tgtgacgcgg caaatccaga agatgaccgg caactttccc atgctcactg tgaattaatt
     1201 gccgatcgcc accattttgc tgccaccagc gctgtagcgc ttgctcgctt tcaggtgtcg
     1261 cacggtcgga tttattggcg acaatgatgt ctgcggcagc cagctggtca cggaagtttt
     1321 cattgctggc gcttttttcg tccagcagca ggcgtggatc gagaatgcac aacgtggcgc
     1381 gcagatctat ccacggttca tagactggtg cggttaacag atcgaggatc tgtttcggat
     1441 ggcccagccc agtcggctct atcaacaagc ggtctggttt tccctgacgc agtaaggtat
     1501 tcaaccctac ctgcatgggt aaaccattaa cgcagcacat gcagccgccg gggatctctt
     1561 tcagcagtgc gccgctatcg gcgagcaaag caccatcaat tccgacttcc ccaaattcat
     1621 taaccagtac cgcccatttt tcgttgggat ctttatgggc taacagatga agaatcgacg
     1681 tggttttccc gctgccgaga aaaccggtga tgaggttggt cctggtcaca acagctccgc
     1741 aatactctgg taagttaact gagcgttaat cctggctaaa aacggcagaa aagagaaggg
     1801 gagagcggac gaaggcgagc ttcatccgct ttaaccgcag aaatcgttaa attttgaggg
     1861 tgtttaatag cgcctgatgc gcgccgaatt gtgcgatttg ttgccgggct tgttcgatgg
     1921 cctgcacaaa atgcgggtta tctggcagat catcaccgaa aacttcacgc agggaaagca
     1981 gggcggtgac gcgttgctca ctgatgctgc ccgcaacaag atcgcgaatt ttatcgctaa
     2041 gcggatcgcg aacatcaatg gcatttccgg catcatcaac gccgctgacg taacgcatcc
     2101 agcctgcaac gcctaatgcc agcaacgacc agtccgtttc gcgccccaga tgtatgcgaa
     2161 tacctgccag catgcgttgc ggtaatttct ggctgccatc catcgcgatt tgccaggtct
     2221 tatgtttcag cgccggatta gcaaaacgtg caattaactt atccgcatat tgtgttaaat
     2281 cgacatcttt aatccgcagt gtcggcgctt gctcatccag cattaatgtt ctggcggcat
     2341 ggcgaaatgc gcgatcctgc atgcaatcac tgatatgggc gaatcctgag aggtaaccca
     2401 gataagcgag aaaagagtgg ctgccattaa gcatccgcag tttcatctct tcccatggca
     2461 ggacatcatt caccatttgt acacctgcga cttcccaggc aggacgccca gcgacgaagt
     2521 tatcttccac cacccactgg ataaacggtt cgcagctaat cgcgcaggga tcatttaccc
     2581 ccagatgctg gctgatttct gccagtgatt cgtcggttgc agccggaaca atgcggtcga
     2641 ccatggttcc cggaaaactg acgtgttctt ttatccaccc ggcgagttct ggcgaacgtt
     2701 tttctgccat tcccagcacc gcgtttttca ccacatggcc attgtcggga atattgtcgc
     2761 aggagagcac ggtaaacggt gtaaggccgc gctcacggcg gcgtttcagt gcttcgacga
     2821 gaatacccgg tgcggagtga ggttcttcag gggtttgtag atcgtgaata atccgcggat
     2881 tgctggtgtc gagtgcaccg gtggccgggt caatacaata gcctttttcg gtaatcgtca
     2941 gggaaacaat tgccacctgt ggctcgcaaa atttctcaat aattgccgct aaggaatcca
     3001 gttttgcatt caggcattcg tgaacggcac cgacaattat cgcctgattg ccgtccgccc
     3061 ctttctccag cacggtatat aaatggttct gtgcgcggag ctggctcatc agttgatcac
     3121 cgctgaacaa gctgatttca cagatccccc agtcgccgcc ctgggcattc agcacacgat
     3181 cggtcagtaa cgcctgatgt gcgcggtgaa aggctccaaa gccaaaatga acgatacgtg
     3241 attgcaactg ctggcgatcg tagcgtggag cgtgtacatg atgcgggagc gtaacggagg
     3301 cgattgtatt cataggagtc atgtcttagt aacgattaat taacaatgac agtgtaaagt
     3361 tatatgacag caattgaaat tggtctggat tgttaatgcg atgaagtgtg agggcgatca
     3421 atcaacgagt gaaaattgag ggcgtaaggt gttcaggaag cgcaaaatag aagattggta
     3481 taataaatta aaaaagggac agcctgagct atcccttttc tgcatgcggc tgaagttagt
     3541 cagcacgccc catataacgg cgttcttcga tatggatacg aattttttcg cccgggctta
     3601 agtattctgg tacctgaatc accagaccag tgctcaatgt cgccggtttg ttacgggcgc
     3661 tggcagatgc ccctttgatg cctggtgcgg tttcaacgat ttccagatca acggtctgcg
     3721 gcagctcaag cgccagcagt tgaccgtccc aggtcagcac ctgcatgtcc ggcatgccgc
     3781 cctctggcat aaacagcaac tcttcttcaa tctgatcttt ggtgaaggta tacggggtat
     3841 agtcttcttt atccataaag acatattcgt tgccatcgac ataggagaag tcaacgtaac
     3901 ggcgggtcag cgtcacggtg tcaacgatat catctccttt gaaacgctct tctactttca
     3961 gcccggtacg gacatcagaa aaacgcattt tgtacagcgt agcggcaccg cgggcagtgg
     4021 gcgactgaat atcaatgtcc ttaaccagca gcagtttgcc gttgtaattc agtaccatac
     4081 cttttttaat ttcgttcgct cttggcatcg aaaaattcct gtagtcgaaa atgtcaaaaa
     4141 tatcgcgaca aagtactcgc gacgagcctt tcgggcaagt ggtattcgca cttttgctgg
     4201 tgcaaaaaag gtggtactgt gcgcgctcat caatccggtg gttaacctta agagaacaac
     4261 gtatggaatg ccgtccgggt tgcggggcgt gttgcactgc cccttcaatt tccagcccta
     4321 ttcccggtat gccagatggc aagcccgcca atacgccctg cattcagctt gatgaacagc
     4381 aacgctgtaa aattttcacc tcgccactgc ggccgaaagt ctgtgcaggc ttacaagcca
     4441 gcgctgaaat gtgcggtaac tcgcgtcagc aggcaatgac ctggcttatc gatctggaaa
     4501 tgctgaccgc gccttaaaca tctttaatcc gcagtaagca aaacagagtg gcgataatca
     4561 tcaccatcgc aaaccagaac acagcgtgat aattccagat ctcggcgacg atgcccgcca
     4621 ctgatcccgc gatgatccag cccacgcgcg acgtgttggt atagagcgtg gtggctgaac
     4681 ctgcctgacc gggcatcaga tcctgaaaat agagcatccc gataccgccc agaatgccaa
     4741 taaaaatagc atttagcagc tgcaagccca acagaatgac aggagaatgc gccatcagca
     4801 ttcctgcgta aaaacagacg ccacccacgg cagcaacgcg cattaagaaa cgcttaccca
     4861 gacgtttggc gaaatatccg gcaatcaaca tggtcgggat ttccagcccg gcggcggtcc
     4921 ccatcatcac accggccagt ttctcgggaa gatggagttc gttgataata aatagcggca
     4981 tgttgatgat gtacaggctg ttcgagcccc acatcaatgt acaaatgaca aacagcagca
     5041 gcgtatcgcg acggttacgg cgcggcgctt caaccgtgcc ggtcgccagc ggaagctctt
     5101 tttgcatcga cggtaaaaac agccacacca tcacgccgca aacaataaat gctaccgctg
     5161 cgctcagata cattaccgta aagctgaaac ccatcgctaa ggcataagcc agcggtgggc
     5221 caatgaccca tgccagtgaa acctgagcgc gtaaaaaaga gctgaacatc accgcctcac
     5281 gtccggtttt gtcggcatgt tcacgggcaa gggcaaacat ttgcgggtta gcggtcgagc
     5341 caaagctgct aagaaagacg ccaacgaata gcaaaacaaa gtagttgcga ttccaggcaa
     5401 aaagggtgca ggccagcacg cctaacaggc agcaaaagac aatcagcgat ttgcgatcgc
     5461 cgcgcttatc agagcgcccg gcgagaaact gactcaccag aaccccaatg acagcgctgc
     5521 cggtaaagaa gaatcccacc atcgccggac gggcatgtac ttcatcggta agaaaaatac
     5581 tgagtgtcgg ggtttgcaga gcgcccgcaa taccggtgag aaaggcaacg attaaaaacg
     5641 ccgtcgaggt caggtcaaac gatttcgcgc tggagactgc gggggagtta tgcatgctgg
     5701 tcgatcactg gaagaggaag acgcggagtt tacgctgctt gtcagaaaat aaacagacag
     5761 cgaaatcaaa agcgccacga ataatcaaaa tgccatttca ctgttgctga ttggctgctg
     5821 acgctgctga aatgaaattg ctgataacgg attttcccat cagcaattag gaaaaatgac
     5881 aaaaaattgt gcagcacatc aaacttttgc tcataacttt acggctttcc ttgcgtgctg
     5941 aaacagaagt attatgcttt cttgaaacgt ttcagcgcga tcttgtcttt aaccctaagc
     6001 caggttggcg ctttttttct catagaagct gaatcgtttc aattcagcaa gagaggagaa
     6061 ctatgttcca gttatccgta caggacatcc atccgggcga aaaggccgga gacaaagaag
     6121 aggcgattcg ccaggtcgct gcggcgctgg tgcaggccgg taatgtagca gaaggctacg
     6181 tcaatggcat gctggcgcgc gaacagcaaa cctcaacgtt cctcggcaat ggtattgcta
     6241 ttccacacgg cactaccgac acccgcgatc aggtgctgaa aaccggcgtt caggtatttc
     6301 agttcccgga aggcgtcacc tggggtgacg gtcaggtagc gtacgtagcg atcggtattg
     6361 ctgccagctc ggatgagcat ctgggcctgc tacgccagct gacccacgta ctgagcgatg
     6421 attccgttgc tgaacaactg aagtcagcaa caacagcaga agaacttcgc gcattgctga
     6481 tgggcgaaaa gcagagtgag cagctgaagc tcgacaacga aatgctgacg ctggatatcg
     6541 tcgccagcga tctgctgact cttcaggcgc tgaacgctgc gcgtctgaaa gaggcggggg
     6601 cagttgacgc cactttcgtc accaaagcca tcaatgaaca accgctgaac ctcggacagg
     6661 gtatctggct gagcgatagc gccgaaggca atctgcgtag cgcaattgcg gtaagccgtg
     6721 cggcaaatgc ttttgatgtg gacggcgaaa cggcagccat gctggtgagt gtggcgatga
     6781 atgacgatca gcccattgcg gttcttaagc gtctcgctga tttgttgctc gacaataaag
     6841 ctgaccgctt gctgaaagcg gatgcggcaa cgttgctggc gctgctgacc agcgatgatg
     6901 cgccgaccga cgacgtgtta agcgcggagt ttgtggtgcg caatgaacac ggcctgcatg
     6961 ctcgtccagg taccatgctg gtcaacacca ttaaacaatt taacagtgat attaccgtga
     7021 caaaccttga tggcaccggc aaaccggcaa acggacgtag tctgatgaaa gttgtggcac
     7081 ttggcgttaa gaaaggtcat cgcctacgct ttaccgccca gggtgcagat gctgaacagg
     7141 cgctgaaagc aatcggcgac gctatcgctg ctggtcttgg ggagggcgca taatgagcag
     7201 acgtgttgct actatcaccc ttaatccggc ttatgacctt gttggtttct gcccggaaat
     7261 tgaacgcggc gaagtgaacc tggtgaaaac caccggtctg catgcggcgg gtaaaggcat
     7321 caacgtggcc aaagtattaa aagacctggg aattgatgtc accgttggcg gcttcctcgg
     7381 taaagacaat caggatggtt ttcagcaact gttcagcgag ctgggcattg ccaaccgttt
     7441 ccaggttgta caggggcgca cccgaattaa cgttaagctg acggaaaaag acggcgaagt
     7501 gaccgacttc aacttctcgg gttttgaagt cacccccgcc gactgggaac gctttgtgac
     7561 tgattctctg agctggctcg gtcagttcga tatggtctgt gtcagcggaa gcttaccgtc
     7621 aggcgtcagc ccggaagcgt tcaccgactg gatgactcgc ctgcgtagtc agtgcccttg
     7681 cattatcttt gatagtagcc gtgaagcgtt agtagcaggt ttgaaagcgg caccgtggct
     7741 ggtgaaacct aaccgccgcg agctggaaat ctgggcaggc cgtaaactgc ctgaaatgaa
     7801 agatgtgatt gaagctgcgc atgcgctgcg tgaacaaggt attgcgcatg tcgttatttc
     7861 actgggtgcc gaaggcgcgc tttgggttaa tgcctccggc gaatggatcg ccaaaccacc
     7921 gtcagtcgat gtcgtaagca ccgttggcgc aggggattct atggttggtg gcctgattta
     7981 tggcttgcta atgcgtgaat ccagtgaaca cacactgcgt ctggcgactg ctgttgcagc
     8041 cctggcggta agtcaaagca atgtgggtat taccgatcgt ccgcagttgg ccgcaatgat
     8101 ggcgcgcgtc gacttacaac cttttaactg acagcaggag aggcataatg aaaacgctgc
     8161 tgattattga cgctaatctc ggtcaggcac gcgcctatat ggcgaagacc ctgctgggcg
     8221 cggcggcgcg aaaagcaaaa ctggaaatca tcgacaatcc gaacgacgca gaaatggcga
     8281 ttgttctcgg tgattccatc ccgaatgaca gcgcgctgaa cggtaaaaat gtctggctgg
     8341 gcgatatttc ccgggcagtt gcgcaccctg agctgttcct gagtgaagcc aaaggccatg
     8401 cgaaacctta cactgcgccg gtcgctgcga cagcaccggt tgccgccagc ggtccgaaac
     8461 gcgtagttgc ggtgactgct tgcccgactg gcgtagcaca cacctttatg gcggctgaag
     8521 ccattgaaac cgaagcgaaa aaacgtggct ggtgggtgaa agttgaaacc cgtggttctg
     8581 ttggcgcggg taatgcaatc actcccgaag aagtagccgc agcggatctg gtgattgtgg
     8641 cggcagatat cgaagtggat ctggcgaaat ttgctggtaa accgatgtat cgcacctcta
     8701 ccggtctggc gctgaagaaa accgcgcagg aactggataa agcggttgct gaagcaacgc
     8761 cgtatgaacc ggcgggcaaa gctcaaacgg cgacctctga aggtaagaac gagagtgcag
     8821 gcgcataccg tcacttgctg acgggcgtct cttacatgct gccgatggtc gttgcaggtg
     8881 gtctgtgtat cgcgctttct tttgcttttg gtatcgaagc gtttaaagag ccgggtacgt
     8941 tggcagcggc gctgatgcag attggtggtg gttcagcctt tgcgctgatg gtgccggtac
     9001 tggcaggtta tattgccttc tccattgccg atcgtccggg tctcacgccg ggtctgattg
     9061 gcggtatgct ggcggtcagc accggttctg gcttcattgg cggtattatt gcgggcttcc
     9121 tggctggtta cattgcgaag ttaatcagta cgcaactgaa actgccacag agtatggagg
     9181 cgctgaaacc gatcctgatc attccgctaa tttccagtct ggtggtcggt ctggcgatga
     9241 tctacctgat cggtaaaccg gttgctggca ttctcgaagg gttgactcac tggctgcaga
     9301 ccatggggac tgcgaatgcg gttctgctgg gggcgatcct cggtggcatg atgtgtactg
     9361 acatgggcgg tccggtaaat aaagcagcgt acgcattcgg tgtgggtctg ctgagtactc
     9421 aaacctatgg cccgatggcg gcgattatgg cggcaggtat ggtgccaccg ctggcaatgg
     9481 gtctggcaac aatggtggcg cgtcgcaaat ttgacaaagc gcagcaggaa ggtggcaaag
     9541 ccgctctggt tcttggtctg tgctttattt cggaaggtgc aattcctttt gctgctcgtg
     9601 atccgatgcg tgtgctgccg tgttgtatcg ttggtggggc gctgactggc gcaatctcaa
     9661 tggcgattgg tgcgaaactg atggcaccgc acggtggtct gtttgttctg ctgatccctg
     9721 gcgctattac gccggtactg ggttacctgg tagcaattat tgccggtacg ctggtggcgg
     9781 gtttggccta tgccttcctg aaacgtccgg aagtggacgc agtagcgaaa gccgcgtaat
     9841 aaaaggtgtg ttacagggca gaaatttact gccccgtatc attggatgag cgcaaagaga
     9901 atgaccggtt tgtgaatgta attttatgaa tttaaatggt tttatctttc agtagaaacc
     9961 tgaaacgttc atcaaacact aaggttattt cctcaagggc agattctttt ctgccctttt
    10021 ttagtgttca tgttctgtgc atcccgtcac aaattcctcg cttttgtaat tcaggcattt
    10081 aatcgcattt tgtgagcctg ctcaaatctg cccgctttgc ctctctggcg gcaatttact
    10141 ttacgtaaaa catgcatact atgagcacat gtttaaatat gaacacatgt ttaaaccgtc
    10201 tttgcagtat ctatatcctt gtggattgac agggttggta attttttacc agaggacgtg
    10261 ctatgcgcga aaaggattat gtcgtaatta taggctcggc gaatattgat gtcgccggat
    10321 attcacatga atcattaaat tatgcggatt caaatccagg taaaataaaa tttacgcctg
    10381 gtggggtagg gcgcaatatt gcacaaaacc tggcgttgct gggtaataaa gcctggctac
    10441 tgagcgccgt aggcagtgat ttttatggcc aatcgctgct aacgcaaacc aatcaatctg
    10501 gcgtttatgt cgataaatgc ctgattgtgc cgggagaaaa tacgtcgagc tatttatcat
    10561 tactcgataa taccggtgaa atgctggttg ctataaatga catgaatatt agcaacgcta
    10621 ttacagctga atatctcgca cagcaccgtg aatttattca gagggcaaag gtcattgtcg
    10681 cggactgtaa tatcagtgaa gaggcactgg catggattct ggataatgct gccaacgtac
    10741 ccgtatttgt cgatccggtt tccgcgtgga aatgcgtcaa agtacgcgag cgattaagtc
    10801 aaatccatac tctcaagcca aaccgccttg aagcggaaac cctgagtggg attgcgctgt
    10861 cagggcgtga agatgtggca aaagttgctg cctggttcca tcaacatggc ctgaaccgac
    10921 tggtattgag catgggcggc gacggcgttt attacagcga tatcagcggt gaaagtggct
    10981 ggtctgcgcc gatcaaaacc aatgttatta atgttaccgg agcgggcgat gccatgatgg
    11041 cgggacttgc ttcgtgttgg gtagacggaa tgccgtttgc cgaatctgtt cgtttcgcac
    11101 agggatgttc gtcaatggcg ctctcctgtg aatacaccaa taaccccgat ttatcgattg
    11161 ccaacgttat atcgttagtg gagaacgcag aatgtctgaa ttaaaaattt cccctgaatt
    11221 attacaaatt tccccggaag tgcaggacgc tttaaaaaac aaaaaaccgg ttgtggcgct
    11281 ggaatcgacc attatttctc acgggatgcc gttcccacaa aatgcccaga ccgcaattga
    11341 agtagaagaa actattcgta aacagggcgc agtacctgcc actatcgcca ttattggcgg
    11401 cgtgatgaaa gtgggtttaa gcaaagaaga aattgaatta ctgggtcgtg aagggcataa
    11461 cgtgactaaa gttagtcgtc gcgatttacc ttttgtcgtt gccgcaggaa aaaatggtgc
    11521 aaccaccgtg gcttcaacga tgattattgc ggcgcttgcc ggaattaaag tatttgccac
    11581 cggcggaatt ggtggtgttc atcgaggggc ggaacatacc ttcgatattt ctgccgattt
    11641 gcaagaactg gcaaatacta atgtcaccgt tgtttgtgcc ggggcgaaat ctattctcga
    11701 tttaggatta accactgagt atttagaaac cttcggtgtg ccgttaattg gctatcagac
    11761 taaagcgctg cctgcgtttt tctgccgtac cagcccgttt gacgtcagca ttcgtctcga
    11821 cagcgccagt gaaattgccc gtgcaatggc ggtgaaatgg caaagcgggc tgaacggtgg
    11881 cctcgtggta gcgaacccga tcccggaaca gtttgcgatg ccagaacaca ctatcaatgc
    11941 ggtgatcgat caggcggtag ctgaagctga agcgcagggt gttattggta aagaaagtac
    12001 gccattcctg ctggcgcgcg ttgctgagct gaccggcggt gacagcctga aatccaacat
    12061 ccagcttgtg ttcaacaacg ccattctggc gagcgaaatt gccaaagaat atcagcgtct
    12121 cgcgggttaa tttctgacgg gcagaaagcc gtcgcccgta ctcatcaaac ataccgaaac
    12181 ctggcgctga cgccgggttt actggcatga agggaaaatc attatggaca taatgagaag
    12241 tgttgtgggg atggtggtgt tactggcaat agcatttctg ttgtcagtga ataaaaagag
    12301 catcagtttg cgtacggttg cagccgcact actgctgcaa attgctcttg gtggcatcat
    12361 gctctatttt ccgccgggaa agtgggctgt agaacaggcg gcattaggcg ttcataaagt
    12421 gatgtcttac agtgatgctg gtagcgcctt catttttggt tcgctggtcg ggccgaaaat
    12481 ggatgtcctc tttgacggtg cgggttttat cttcgccttt cgcgtacttc cggcgattat
    12541 cttcgtcact gcgctcatta gtctgctgta ctacattggc gtgatggggc tgctgattcg
    12601 catccttggc agcattttcc agaaagccct caacatcagc aaaatcgaat cttttgtcgc
    12661 agtcaccact attttcctcg ggcaaaatga gatcccggcg atcgttaaac cgtttatcga
    12721 tcgcatgaat cgaaacgagt tgtttacggc tatttgtagc gggatggcgt ccattgctgg
    12781 ttcgatgatg attggttatg ccggaatggg cgtaccaatt gactacctgt tggcggcatc
    12841 gctgatggcg atccccggcg gtattttgtt tgcacgtatt cttagtccag caaccgagct
    12901 ttcgcaggtc acgtttgaaa atctgtcgtt cagcgaaacg ccgccaaaaa gctttatcga
    12961 agcagcggcg agcggtgcga tgaccgggct gaaaatcgcc gctggtgtgg cgacggtggt
    13021 aatggcattt gtggcaatta ttgcgctgat caacggcatt atcggcggaa ttggcggctg
    13081 gtttggcttc gccaatgctt ctctggaaag tatttttggc tatgtgctgg caccgctggc
    13141 gtggatcatg ggtgtggact ggagtgatgc caaccttgcg ggtagcctga ttgggcagaa
    13201 actggcgatt aacgaattcg tcgcttacct gagtttctcc ccatacctgc aaacgggcgg
    13261 cacgctggaa gtgaaaacca ttgcgattat ctcctttgcg ctttgtggtt ttgctaactt
    13321 tggttctatc ggtgttgtcg ttggggcatt ttcggctatt tcgccaaaac gcgcaccgga
    13381 aatcgctcag cttggtttac gggcgctggc agcagcaacg ctttccaacc tgatgagcgc
    13441 gaccattgcc gggttcttta ttggtctggc gtaatgctca atttgtagac cggataaaac
    13501 gcatcaagcg ttgcatccgg caatcgcgct attgcaaata atttttactg catcatcccg
    13561 gagaatttat tctccgggtc catctccagt gccagccctg acaactgctt tctgttttta
    13621 attagatatc ctttcttgct tttaattaat aaaccgtcgt gaatgaattg cgcgagaaca
    13681 tataaaagat gtcgataaga aacgcctaaa tactctgccg cttgcgtatg cttttcgtga
    13741 taaagatcac cttcttgtga gagtaatata aatgctgcca ggcgattcac taacggaaat
    13801 gattgattct gagttaaaga aacaatatta cgataatttt tatgacttaa ggtgacgcaa
    13861 agttttcgta aaaatagggt gtcgtttaat aacagcggac ggtaatgttt catagggagc
    13921 gcaaggcacc aacactcttc aatagcctgt accgcacgcg gttcatggtc tttatcgatt
    13981 aactcaatct caccaataaa acagggggcg gcaaagaaat cgatcagcga cacgcgacca
    14041 ttagctagcg tggcgtaaag cctggcgcgg cctcgcgtca ggtaaaacag ccaggacggc
    14101 tgttgccctt cctgcacaat gtagtcacgc gctaaaaaat gaaacagccg cgtatcggct
    14161 gaaacatccg ttaaaaagca atccttaaac gcggattcac tcatcaattg ctgcttaaga
    14221 tcattattgt ggatttcttt cacgatgttt atccgctatg agatttctca tatttagcat
    14281 acgtacgctg ttgttaagat tcaatcatcg gcccgatcga aatttctttg ctgtagaaaa
    14341 aataacgcaa ctggaaacag aggaaataaa caatggaaaa gagaaaaatt attctggatt
    14401 gtgatccggg tcatgatgat gctattgcta taatgatggc ggcgaaacat ccggcaatag
    14461 atttattagg catcactatt gtagcgggta atcagacgct tgataaaaca ttaattaatg
    14521 gcctgaatgt ttgccagaaa ctggagatta atgttccggt ttatgcgggg atgccacaac
    14581 ccattatgcg gcaacaaatc gttgccgata atattcacgg tgataccgga ctggatggcc
    14641 cggtattcga gccgctgacc cgccaggcag aaagcactca tgcggtgaaa tacatcattg
    14701 ataccctgat ggcaagcgat ggcgatatca ctctggtgcc ggttggtccg ctttcaaata
    14761 tcgcggtggc aatgcgtatg caacccgcga tcctgcccaa aatccgtgaa attgtgctaa
    14821 tgggcggcgc ttacggtaca ggcaacttca cgccatctgc cgagttcaac atctttgctg
    14881 acccggaagc cgcacgcgtg gtgttcacct ctggcgttcc attagtgatg atgggcctcg
    14941 atctcaccaa ccagaccgtt tgcaccccgg acgtgattgc acgaatggaa agggcaggcg
    15001 gccctgccgg agagctgttc agcgacatca tgaacttcac tctcaaaacc cagttcgaaa
    15061 actacggcct ggctggcggc ccggtgcacg acgccacctg catcggttat ctgattaacc
    15121 ctgatggcat taaaacccag gagatgtacg tcgaagtgga cgtcaacagt ggcccttgtt
    15181 atgggcgtac cgtctgcgac gagctgggcg ttcttggcaa gcccgctaat accaaagtcg
    15241 gcatcactat tgatagcgac tggttctggg gcttagtcga agagtgcgtg cgcggctaca
    15301 tcaaaaccca ttaagtgttg ataacaagcc gggccggtat cgcaagatgc aggcctggct
    15361 atttctgatg ggcagaaagc cgtcgcccaa tatgacatga agagataatc actatggatg
    15421 tcatgagaag tgttctggga atggtggtat tgctgacgat tgcgttttta ctgtcagtaa
    15481 acaagaagaa gatcagcctg cgtaccgttg gcgcggcgtt agtgttacag gtcgtgattg
    15541 gcggcattat gctttggtta ccgccagggc gttgggtcgc tgaaaaagtc gcttttggtg
    15601 tgcataaagt gatggcgtac agcgacgcgg gtagcgcatt tatcttcggt tctctggtcg
    15661 gaccgaaaat ggacacctta tttgatggcg caggatttat ctttggtttc agggtattac
    15721 cggcaattat cttcgtcact gcactggtga gtattctcta ctacatcggt gtgatgggga
    15781 ttttaattcg cattctcggc ggtatattcc agaaagcatt aaatatcagc aagattgagt
    15841 cattcgtcgc ggtcactacc attttcctcg ggcaaaacga aattccggcg attgtgaagc
    15901 cctttatcga tcggctgaat cgcaatgaat tatttacagc gatttgtagc ggcatggcct
    15961 cgattgctgg ttcgacaatg attggttatg ccgcactggg cgtgcctgtg gaatatttgc
    16021 tggcggcatc gttaatggcg atccccgggg ggatcttgtt tgcccgcctg ctaagcccgg
    16081 ctacggaatc ttcgcaggtt tcttttaata acctctcttt caccgaaaca ccgccaaaaa
    16141 gcattattga agccgccgcg acaggggcaa tgaccgggct gaaaatcgcc gcaggtgtgg
    16201 cgacagtagt gatggcattc gtcgccatca ttgcgttaat taacggtatt atcggcggcg
    16261 ttggcggctg gtttggtttt gaacatgcct cgctggagtc cattgtaggt tatctgctgg
    16321 ccccactggc gtgggtaatg ggtgttgact ggagtgatgc gaatcttgcc gggagtttga
    16381 ttggacagaa actggcaata aatgaatttg tcgcttatct caatttctca ccctatctgc
    16441 aaacggctgg cactctggat gctaaaaccg tggcgattat ttccttcgcg ttgtgcggtt
    16501 tcgctaactt tggttctatc ggggtggtgg tgggggcgtt ttctgcggtt gcgccacacc
    16561 gtgcgccgga aatcgcccag cttggtttac gcgcgctggc ggcggcgaca ctttctaacc
    16621 tgatgagtgc caccattgcc gggttcttta ttggtttagc gtagtgtgcg atttatgagc
    16681 cggataagat gcggtcaagc gtcgaatccg gcaacattgt tacaccattg gcactaatga
    16741 aagcgcgtta tcggcagaca gggtagggtt gtttaagctg ccgctggcgc gtgaaattgc
    16801 cgcgcaggcc atcgcaaaac gggcgctgtc acggaaattg tttccttcca gaaagctata
    16861 taccaggccc gccataaaac catcgtcagc accaaaactg tctactgtcg tatgcgctgg
    16921 tgcggtcagc aaaaattgtt ctccatcctt ttcgctgcaa taaactgact catcgggcaa
    16981 ataaacaaac agttgctgaa caccttgctg atgcaacgca ttcactgcag cattacggtc
    17041 agcatcgctg gtgatcgcct gtccccataa aatttccagc tccggtaaag tgggtttcag
    17101 ggtgtgaata tgcgccagcc agtgtttgat tttgcccgct ttgaattctg aaacggtatc
    17161 gacaaacacc gggatctcac cagcgagggt aaagacccat tccagcgcct cggctgtcag
    17221 gttgcaatca gccagtacca cgcccgcatg acgaagtaaa tcgcgcgacc cgttcaataa
    17281 ttgcggcgat aactgttcca gcagatgggt atcgttaatc gccagcacgg tttcatcgtc
    17341 tcgattggca attgccagat acgtcgatgt gctttgacca tgtaaacgaa cgcagccgga
    17401 gacattcacg ccggcgcggc gcgtttcttc caggagcatt tcgccataaa agtcatcgcc
    17461 aatcactgaa agcaaatgga cgtcacggcc taacagcgcc agattgtggg cgatgttgcg
    17521 tcccacgccg cccgctgagc aatgaattgt accgggatga gaagccgatt gcgggtaacg
    17581 gatatccgcc atcccgcgaa tatccatatt gattgtcccc accactacgc agtattcctg
    17641 ctcggtgaga atgtaacctt tgcctttaat ccggcctttg cgcattaaat ccataatatg
    17701 cgccgcaacg cgcgaacggc tgatttgcag catgtccgca atttcgttct gctgaatcag
    17761 cgggttacgc cgtaaaattg caaggatctc cttttcccgg ttattcatct tcaggctacc
    17821 gctttttcag tttgttgcgc tttcagccag gcgatctctt ctgcccagat atccgggttg
    17881 atggtttcga ggatcagcgg aatgccgtcg aaacggtcgt cctgcatgat ccagcggaac
    17941 gcatcatgac cgatattgcc ttcaccgagg ctatgatggc ggtcaacgcg gctgccaaag
    18001 gtgcttttcg catcgttaag gtgcatcccg cgcagatact taaagccgac agtacgggca
    18061 aaatccgcga atgttttctc gcattcggct ggagtacgca aatcataccc ggcagcgaaa
    18121 gcatggcagg tatcaatgca gacgccgacg cgggatttat cttccacgcc gtcgataatc
    18181 gccgcgagat gttcgaattt aaaccctaag ttactgccct gaccggcggt gttttctatc
    18241 accgctgtca caccttgagt tttatccagc gcaatgttga tggattcggc aatacgcgca
    18301 aggcaatcct cttctgaaat ctgcatcaga tggctgccag ggtggaagtt gagcaaagaa
    18361 agccccagct gttcgcaacg ctgcatttca tctataaagg catcgcgcga tttttccaga
    18421 gcttcagtga ccggatgtcc gaggttaatc agataactgt cgtggggaag aatttgcgcc
    18481 gatgtgtagt gatatttttc acaggcggct ttgaattcat cgatggtttg cgtcgtgagc
    18541 ggtgcggcac gccactgacg ttggtttttg gtgaacaagg caaacgcggt tgcgtcgatt
    18601 tcggcggcgc gaattgcggc atttgccaga ccgccagcag cactaacgtg cgctccaatg
    18661 tatttcatgc gaggactcct gttaaacccg ctggaggaaa acggtaatga tagcgggtta
    18721 acaggagcaa gatgtagtgg tttatgcgat gacgctttga atcacatagt taatcgcacc
    18781 accaccaaca atcagccagg caaacagtac cagtgccatc agcagaggtt tcgccccagc
    18841 ttttttcagc gcgctgacgt gagtggtcag acccagcgcc gccatcgcca ttgccagcag
    18901 gaaggtatcc agcgttacca gcatgttcac cacgctctgc ggtaacaggt ggaacgagtt
    18961 aaagatggca actacgatga acaagatggc aaaccacgga atagtgattt tgcttttctc
    19021 gccgctgttc gccccagaca gctgtttaac acgcgccgcc agcaggatga ggaacggagc
    19081 cagcatcatc acgcgcagca ttttggaaat aactgctgcg ttttccgcat ccgggctgat
    19141 ggcatgacct gccgccacca cctgcgccac ttcgtgcaca gtagaaccaa tgtagatacc
    19201 gaaagtttcc ggactaaacc attgagacat cagcggatat atcgccgggt agaggaaaat
    19261 cgcgacggtc ccgaagataa caacggttgc aacagccacg gttactttac tggcttccgc
    19321 tttcactacc ggctcagtcg ccagtaccgc ggcagcacca cagatactgc taccggcacc
    19381 gatcaaccag ctggtgtgct tatccagacc aaacactttc tgccccagga agcaagccag
    19441 caggaaggta ctggacagcg tcagcacgtc aatgatgatc ccactgatac cgacatcggc
    19501 aatttgcgag aacgtcagac ggaagccata aagaatgata cccagacgta ataaatattg
    19561 cttggcaaac agcacaccac cgtcacagct tttccagatg tgcggataga tggtgttgcc
    19621 taaaaccatc cccaacaaga ttgcgagggt gagggcacta aacccggcac ccgcaaccgc
    19681 gggaatggaa ccaccccaca gggcgacccc ggtgataact gcactcaggg ctaaccccgg
    19741 aataaaatgc cacagtgtac gatgttgttt ctgtaaggtg atattcgtca taaccctctc
    19801 ctttaaccgg gcataaggtt acgactaact ggtttaaaaa taaaattgat tatatattta
    19861 taattaatct ttataagtgg taagcgacta tgcacatcac cctccggcag ttggaagttt
    19921 ttgcagaagt attgaaaagt ggatcaacca cccaagcgtc ggtgatgctg gcgttgtcgc
    19981 aatcagcagt gagcgcagcc ttgaccgacc tggaagggca gcttggcgtg caactgtttg
    20041 atcgcgtggg gaaaagactg gttgttaatg aacacgggcg gctgctctat ccgcgtgcgt
    20101 tggcattgct tgaacaggcg gttgaaatcg aacaactgtt tcgcgaagac aacggcgcga
    20161 ttcgtatcta tgccagtagt accatcggta actacattct gcctgcagtt atcgcccgtt
    20221 atcgccatga ttatccgcag ttgccgattg aacttagcgt tgggaatagc caggacgtga
    20281 tgcaagcggt gctggatttc cgcgttgata ttggctttat tgaaggaccg tgccacagca
    20341 ctgaaatcat ttctgaaccg tggctggaag acgagctggt ggttttcgcc gcgccgactt
    20401 cgccgttggc ccgtggtccg gtcaccttag aacagctggc cgctgcgccg tggatcctgc
    20461 gtgaacgcgg ttccggcacg cgggagattg tcgattatct gttgctgtca catttaccga
    20521 agtttgagat ggcgatggaa ttaggtaact ccgaggcaat caaacatgcg gtgcgtcatg
    20581 ggttgggaat tagttgcctg tcgcgacgtg tgattgaaga tcaattgcag gcaggcacat
    20641 taagtgaagt tgcggtccct ctgccgcgcc tgatgcgtac gttgtggcgt atacatcatc
    20701 ggcaaaaaca cctttccaac gcgctacggc gctttctgga ctattgcgat cccgcaaatg
    20761 tgccgcgtta agttgctgta caagaacatg ctggtgctgt gtcgatttcg tgacgcagcg
    20821 ccttcagcat gcattcgcca gaaaagagat tggctgcttt acttataatc cctgggcgat
    20881 catgaaggtg tcttataacc gtgtatttct gccggaagga ttgccaatcg tctgctacaa
    20941 tcgcgcctca ttttttagat ggatagcatt tttgtatggt ttccgaaact aaaaccacag
    21001 aagcgccggg cttacgccgt gaattaaagg cgcgtcacct gacgatgatt gccattggcg
    21061 gttccatcgg tacaggtctt tttgttgcct ctggcgcaac gatttctcag gcaggtccgg
    21121 gcggggcatt gctctcgtat atgttgattg gcctgatggt ttacttcctg atgaccagtc
    21181 tcggtgaact ggctgcatat atgccggttt ccggttcgtt tgccacttac ggtcagaact
    21241 atgttgaaga gggctttggc ttcgcgctgg gctggaacta ctggtacaac tgggcggtga
    21301 ctattgccgt tgacctggtt gcagctcagc tggtcatgag ctggtggttc ccggatacac
    21361 cgggctggat ctggagtgcg ttgttcctcg gcgttatctt cctgctgaac tacatctctg
    21421 ttcgcggctt tggtgaagcg gaatactggt tctcactgat caaagtcacg acagttattg
    21481 tctttatcat cgttggcgtg ctgatgatta tcggtatctt caaaggcgcg cagcctgcgg
    21541 gctggagcaa ctggacaatc ggcgaagcgc cgtttgctgg tggttttgcg gcgatgatcg
    21601 gcgtagctat gattgtcggc ttctctttcc agggaaccga gctgatcggt attgctgcag
    21661 gcgagtccga agatccgggg aaaaacattc cacgcgcggt acgtcaggtg ttctggcgaa
    21721 tcctgttgtt ctatgtgttc gcgatcctga ttatcagcct gattattccg tacaccgatc
    21781 cgagcctgct gcgtaacgat gttaaagaca tcagcgttag tccgttcacc ctggtgttcc
    21841 agcacgcggg tctgctctct gctgcggcgg tgatgaacgc agttattctg acggcggtgc
    21901 tgtcagcggg taactccggt atgtatgcgt ctactcgtat gctgtacacc ctggcgtgtg
    21961 acggtaaagc gccgcgcatt ttcgctaaac tgtcgcgtgg tggcgtgccg cgtaatgcgc
    22021 tgtatgcgac gacggtgatt gccggtctgt gcttcctgac ctccatgttt ggcaaccaga
    22081 cggtatacct gtggctgctg aatacctccg ggatgaccgg ttttatcgcc tggctgggga
    22141 ttgccattag ccactatcgc ttccgtcgcg gttacgtatt gcagggacac gacattaacg
    22201 atctgccgta ccgttcaggt ttcttcccat tggggccgat cttcgcattc attctgtgtc
    22261 tgattatcac tttgggccag aactacgaag cgttcctgaa agataccatt gactggggcg
    22321 gcgtagcggc aacgtatatt ggtatcccgc tgttcctgat tatttggttc ggctacaagc
    22381 tgattaaagg aacccacttc gtacgctaca gcgaaatgaa gttcccgcag aacgataaga
    22441 aataagtttc tcccttcctt gctaagccct ctcaaccgag agggcttttt caattccatt
    22501 tccctgacaa atcatgcgga tataaaattt aacatttgga ttgataattg ttatcgtttg
    22561 cattatcgtt acgccgcaat caaaaaaggc tgacaaatca gaggctggtc cggctttctg
    22621 ggatgatcac ctgcatacaa ataagtccac cgcgatgctg ccgtacgcaa ggggacgtga
    22681 agaagatgtg agcgataacc cattttattt tcgtagttac ctcatggaga tatggaatgt
    22741 ttaggttgaa ccctttcgta cgggtcgggc tgtgtttgtc cgctatttct tgtgcatggc
    22801 ctgtgttagc ggtcgatgac gatggcgaaa cgatggttgt cactgcatct tccgttgaac
    22861 aaaaccttaa agatgcaccc gccagtatca gcgtcattac ccaggaagac ctgcagcgaa
    22921 aaccggtaca gaatctgaag gatgtcctca aagaagtgcc tggcgtacaa ctgacgaacg
    22981 aaggggataa ccgtaagggc gttagtattc gtggtctgga cagcagctac accctgattc
    23041 ttgtcgacgg taaacgcgtt aactcccgca atgccgtctt ccgccacaat gatttcgatc
    23101 tgaactggat cccggtcgat tccatcgaac gtattgaagt ggtccgtggc ccgatgtcgt
    23161 cgctgtacgg ttccgatgcg ctcggcggtg tagtgaatat catcaccaaa aaaatcggtc
    23221 agaaatggtc gggtaccgtt accgtcgata ccaccattca ggaacatcgc gatcgcggtg
    23281 atacctataa cggtcagttc tttaccagtg gaccattaat tgatggtgtg ctgggaatga
    23341 aagcttacgg cagcctggca aaacgtgaaa aggatgaccc gcaaaactca acgaccaccg
    23401 ataccggaga aacgccgcgt attgaaggat tctccagccg cgacggcaat gtcgaatttg
    23461 cctggacacc gaatcaaaat cacgatttta ctgccggata cggtttcgac cgtcaggatc
    23521 gtgattccga ctcgctggac aaaaaccgcc tggaacgcca gaactactcc gtcagccata
    23581 atgggcgttg ggattacggc accagcgaac tgaaatacta cggtgagaaa gtcgagaaca
    23641 aaaaccctgg caacagcagc ccgataactt ccgaaagcaa tacggtcgac ggcaaataca
    23701 cgttgccgct gacggcgatt aatcagtttc tcacggttgg cggtgaatgg cgtcacgaca
    23761 aacttagcga tgcggtgaac ctgaccgggg gaaccagctc caaaacgtct gccagccagt
    23821 acgcgctgtt tgtggaagat gaatggcgga tcttcgagcc gctggcgctg acgaccggcg
    23881 tgcgtatgga cgatcacgaa acctacggtg aacactggag tccgcgtgcc tacctggttt
    23941 ataacgccac cgacaccgta acggtgaaag ggggctgggc gacggcattt aaagcacctt
    24001 ctctgttgca acttagccct gactggacga gcaattcctg ccgtggcgca tgtaagattg
    24061 tgggtagccc ggatctgaaa ccagaaacca gcgaaagttg ggagctgggg ctttactaca
    24121 tgggtgaaga aggctggctg gaaggggttg aatccagcgt taccgttttc cgtaacgatg
    24181 tgaaagatcg tatcagcatc agccgtacgt ctgacgtcaa tgctgcaccg ggctaccaaa
    24241 actttgttgg ttttacagcg aacggtgtgc cggtatttag ctactacaac gttaacaaag
    24301 cacgtattca gggcgtggaa accgagctga aaattccgtt caacgatgaa tggaaactgt
    24361 cactcaacta cacctacaac gatggtcgtg atgtcagcaa cggcgaaaac aaaccgctat
    24421 ccgatctgcc gttccatacc gctaacggta cgctggactg gaaaccgctg gcgctggaag
    24481 actggtcatt ctatgtttct ggtcactata ccgggcagaa acgagccgac agcgcgacgg
    24541 ctaaaacacc gggcggttat accatctgga ataccggcgc ggcctggcag gtgactaaag
    24601 acgtcaaact gcgcgcaggc gtgctgaacc ttggcgacaa ggatctcagt cgtgacgact
    24661 acagctataa cgaagacgga cgtcgttact ttatggcagt ggattatcgc ttctgatgag
    24721 aagatgcccg gcgaaccggg cggactttca cttcagtaaa tactgcgcat ggaagcgcag
    24781 gtgatcctct ataaaagagg cgatgaagta gtaactgtga tcatatcccg gctgaatacg
    24841 cagcgtcatc ggccatgctt tctggcgcgc ggcttctgca agtactgcag gttgcagttg
    24901 gtcagcaaga aactgatcgt tatcaccctg atcgataagc gtcgggatcg catcctgcgc
    24961 gttactggca tacatcagcg cgcaactgtc ccattccagc catgcatttt tgtcctcacc
    25021 taaatagcgg ctaaacgctt tgattcccca cgggacgctg cacggattca caattggcgc
    25081 aaaggccgaa acgctggtgt atttgcccgg gtttttcagc gccataatca gcgcaccgtg
    25141 accacccatt gaatgaccgc taatagcgca gcggtcgctg acattaaatt gcgactgaac
    25201 cagcgccggt aattcatcac gcagataatc atacatccgg taatgcgtcg cccacggcgg
    25261 ttgcgtggca ttaagataaa agcctgcgcc ctggcctaaa tcgtagccat cgtcgttggc
    25321 aaccttttcg ccgcgcgggc tggtgtctgg cataaccagt acaatcccca gttccgccgc
    25381 cacgcgctgg gcacccgcct tggtggtgaa gttctcgtca ttgcaggtta atccggaaag
    25441 ccagtacagc actggtggcg gggtgtgatc acgagggggt gggagaaaga tactgaacgt
    25501 catcgggcag tttaaggtac tggagtcgtg tcgccagcgt tgctgccagc cttcaaaaca
    25561 gcggtgctct tcgagcattt ccatgcgtgg ctccttgttg tgttgtttgc aatttcttca
    25621 tcataccgtt tattcaatgt gctgtgagta actttcactt ccgtatttgc ataacgatgt
    25681 tttaacatct gctgatgaaa ggcagcggca attacaataa ttatcgctgt gaatactgga
    25741 ttatgtgcgc cgcctcacgc acaataatca ggctgtaaat cagcttaata actttgcccc
    25801 cacgcagggc ggaggcgtca cacctgcagg agaaatcata aatgccatca ctcagtaaag
    25861 aagcggccct ggttcatgaa gcgttagttg cgcgaggact ggaaacaccg ctgcgcccgc
    25921 ccgtgcatga aatggataac gaaacgcgca aaagccttat tgctggtcat atgaccgaaa
    25981 tcatgcagct gctgaatctc gacctggctg atgacagttt gatggaaacg ccgcatcgca
    26041 tcgctaaaat gtatgtcgat gaaattttct ccggtctgga ttacgccaac ttcccgaaaa
    26101 tcaccctcat tgaaaacaaa atgaaggtcg atgaaatggt caccgtgcgc gatatcactc
    26161 tgaccagtac ctgtgaacac cattttgtta ccatcgatgg caaagcgacg gtggcttata
    26221 tcccgaaaga ttcggtgatc ggtctgtcaa aaattaaccg tattgtgcag ttctttgccc
    26281 agcgtccgca ggtgcaggaa cgtctgacgc agcaaattct cattgcgcta caaacgctgc
    26341 tgggcaccaa taacgtggct gtctctatcg acgcggtgca ttattgcgtg aaggcgcgtg
    26401 gcatccgcga tgcaaccagt gccacgacaa cgacctctct tggtggattg ttcaaatcca
    26461 gtcagaatac gcgccacgag tttctgcgcg ctgtgcgtca tcacaactga tttaaaggca
    26521 ggaaccatgg agcgcaacgt cacgctcgac tttgttcgcg gcgtcgccat tctggggatc
    26581 ctgctattaa acatcagcgc ctttgggcta ccaaaggcgg cttatcttaa tcccgcctgg
    26641 tacggcgcta ttacgccgca ggatgcatgg acctgggcat ttctcgatct catcggccag
    26701 gtgaaattcc tcacgctttt tgcgctgctg tttggtgctg gcctgcaaat gttgctgccc
    26761 cgtggcagac gctggatcca gtcgcggtta acgctgttag tcttgctggg ctttattcac
    26821 ggtttattgt tctgggacgg cgatattctg ctggcttacg ggctggtggg cttaatctgc
    26881 tggcggctgg tgcgcgatgc gccatcggta aaaagccttt ttaataccgg cgtcatgctt
    26941 tatctggtgg ggcttggcgt tttgctgtta ttggggttga tttccgatag ccagactagc
    27001 cgcgcctgga cgccggatgc atcggctatt ttatatgaaa aatactggaa gcttcacggc
    27061 ggcgttgaag cgatcagtaa tcgtgccgat ggtgttggga acagtttatt ggcactgggc
    27121 gcacagtatg gctggcaact ggctgggatg atgctcattg gtgccgcatt gatgcgcagt
    27181 ggctggctga aagggcagtt cagcttacgt cactatcgtc gtactggttt tgtgctagtg
    27241 gcgattgggg tgatcattaa ccttcctgcc atcgccctgc aatggcagct ggactgggcg
    27301 tatcgctggt gtgccttctt acttcaaatg ccgcgggaac tgagtgcgcc gtttcaggcg
    27361 attggctatg cgtcgctgtt ttatggcttc tggccgcaat tgagccgctt taagctggtg
    27421 ctggcgatcg cctgcgtcgg acggatggcg ctgaccaact atctattgca aacgctgatt
    27481 tgtaccacgc ttttttacca cctcggtctg tttatgcagt ttgaccgtct ggaattgctg
    27541 gcgtttgtta ttccggtatg gctggcgaat attctcttct ctgttatctg gctgcgttac
    27601 ttccgccagg ggccggtgga atggctctgg cgtcagttaa ctttgcgtgc tgccggaccg
    27661 gcaatatcta aaacatcaag ataacgatct ggatcacatt cgttaacaaa acggctgtaa
    27721 ccgtttccat tgctgtgact cgattcacga agtcctgtat tcagtgctga caaaatagcc
    27781 ggcagcaagc agtcatttac tgcaatctca taacaggtag tgaatatgat caccattcgt
    27841 gatgtagcgc gtcaggctgg cgtctctgtg gcaacggttt cccgggtgct caataacagc
    27901 acgctggtca gtgccgacac gcgtgaagca gtaatgaaag ccgtgagtga gctggattat
    27961 cggccaaacg ccaatgccca ggcgctggca actcaggtta gcgacaccat tggcgtggtg
    28021 gtgatggacg tttctgatgc gtttttcggc gcgctggtaa aagcggtgga tctggtcgct
    28081 cagcagcatc agaaatacgt gctaatcggc aatagctatc atgaagcgga aaaagagcgt
    28141 cacgccattg aggtgttaat tcgccagcgt tgtaatgcgt tgattgttca ctcaaaagca
    28201 ttgagtgacg atgaactggc gcaatttatg gataacattc ccggtatggt gttaatcaac
    28261 cgcgttgtgc cggggtacac ccatcgttgc gtttgcctgg ataatctcag cggtgcccga
    28321 atggcgacgc gcatgttgct gaataacggt catcaacgta ttggttatct ttcttccagc
    28381 cacggcattg aagatgacgc catgcgtaaa gcaggctgga tgagtgcgtt gaaagagcag
    28441 gatattattc cgccggaaag ctggattggc actggtacgc cggacatgcc gggcggtgag
    28501 gcggcgatgg ttgaactgct ggggcgcaat ctacaactta ccgctgtatt tgcttataac
    28561 gacaatatgg ctgctggcgc actgacagca ttaaaagata atggcattgc gattccgtta
    28621 catctctcaa tcatcggttt cgatgatatt cccatcgccc gttacaccga cccgcaatta
    28681 acgaccgtgc gttatcccat tgcttcaatg gcgaaattag ccaccgaact ggccttgcag
    28741 ggggcagcag gcaatattga tcctcgtgcc agccactgtt ttatgccgac gttagtgcgt
    28801 cgccattctg tcgcaacacg ccagaatgcg gcggcgatca ctaactcaac aaatcaggcg
    28861 atgtaaccgc tttcaatctg tgagtgattt cacagtatct taacaatgtg atagctatga
    28921 ttgcaccgtt ttaacgttgt aacccgtatg taacagtgaa taatcacttt caccgaggta
    28981 atgcgtcata acaacaatta aagccgtttt ctggagcgtt accgggcatg gaagaacgaa
    29041 ttttaaaaag tgagcttcgg cgttcagtaa cacttcatta actctactgc cccgccgagc
    29101 atttatctca agcactaccc tgcataagaa aaaccggaga taccatgaat aagaaggtgt
    29161 taaccctgtc tgctgtgatg gccagcatgt tattcggtgc cactgcacac gctgctgata
    29221 ctcgcattgg tgtaacaatc tataagtacg acgataactt tatgtctgta gtgcgcaagg
    29281 ctattgagca agatgcgaaa gccgcgccag atgttcagct gctgatgaat gattcacaga
    29341 atgaccagtc caagcagaac gatcagatcg acgtattgct ggcgaaaggg gtgaaggcac
    29401 tggcaatcaa cctggttgac ccggcagctg cgggtacggt gattgagaaa gcgcgtgggc
    29461 aaaatgtgcc ggtggtattc tttaacaaag aaccgtctcg taaggcgctg gatagctacg
    29521 acaaagccta ctacgttggc actgactcca aagagtccgg cattattcaa ggcgatttga
    29581 ttgctaaaca ctgggcggcg aatcagggtt gggatctgaa taaagacggt cagattcaat
    29641 tcgtactgct gaaaggtgaa ccgggccatc cggatgcaga agcacgtacc acttacgtga
    29701 ttaaagagct gaacgacaaa ggcattaaaa ctgaacagtt acagttagat accgctatgt
    29761 gggataccgc tcaggcgaaa gataagatgg acgcctggct gtctggcccg aacgccaaca
    29821 aaatcgaagt ggttatcgcc aacaacgatg cgatggcaat gggcgcggta gaagcactga
    29881 aagcacacaa caagtccagc attccggtgt ttggcgtcga tgctctgcca gaagcgctgg
    29941 cgctggtgaa atccggtgca ctggcgggca ccgtactgaa cgatgctaac aaccaggcga
    30001 aagcgacctt tgatctggcg aaaaacctgg ccgatggtaa aggtgcggct gatggcacca
    30061 actggaaaat cgacaacaaa gtggtccgcg taccttatgt tggcgtagat aaagacaacc
    30121 tggctgaatt cagcaagaaa taagactgat tgggcgcagt ctattactgc gcccttattg
    30181 tatggccaac aaggtaaaat tatatggtca gctcaacgac tccgtcttcc ggggaatact
    30241 tgttggaaat gagcggtatc aacaagtctt ttcctggtgt taaggcactt gataacgtta
    30301 atttaaaagt ccggccacat tctatccatg cattaatggg ggaaaacggc gcaggaaaat
    30361 caacattatt aaaatgcctg tttggtattt ataaaaaaga ctccggcacc attttattcc
    30421 agggtaaaga gatcgatttc cattcggcca aagaagcact ggaaaatggt atttcgatgg
    30481 tacaccagga gttaaacctg gtattacaac gttcggtgat ggacaacatg tggcttgggc
    30541 gataccccac caaaggcatg tttgtcgatc aggacaaaat gtaccgcgaa accaaagcga
    30601 tttttgatga actggatatt gatatcgatc cgcgtgcgcg cgtcggcaca ttatctgttt
    30661 cgcaaatgca gatgatcgaa atcgccaaag cgttttccta taacgcgaaa attgtgatta
    30721 tggatgaacc gacttcttcg ttaaccgaaa aagaggtcaa tcatctgttc actattattc
    30781 gtaaattaaa agagcgcggc tgcggtattg tttatatctc gcataaaatg gaggaaatct
    30841 tccagttatg tgatgaagtt accgtattgc gcgacggtca gtggatcgcc accgaaccgc
    30901 tggcagggct gacgatggac aagattatcg ccatgatggt tgggcgttct cttaaccagc
    30961 gttttcctga caaagaaaac aagccgggcg aagtcatcct cgaggtacgt aacctgacgt
    31021 cactgcgcca gccgtcgatt cgcgatgtct cgtttgatct gcataaaggg gagatcctcg
    31081 gtattgccgg tctggtgggg gcgaaacgta ccgatattgt tgagacgtta tttggtattc
    31141 gcgagaaatc ggctggcacc attacgttgc acggcaaaaa gatcaataac cataatgcca
    31201 acgaagccat aaaccacgga tttgcactgg taactgagga gcgccgctca acgggaattt
    31261 atgcctatct ggatattggt tttaactcgt taatttccaa tattcgcaac tacaaaaata
    31321 aagttggttt actggataac tcgcggatga aacgcgatac ccagtgggtg attgattcga
    31381 tgcgggtaaa aacaccgggg catcggacgc aaattggttc gctctcaggt ggtaatcagc
    31441 aaaaggtgat tattgggcgc tggctgttaa cgcaaccaga aatattaatg ctcgatgaac
    31501 cgacgcgcgg tattgatgtt ggggcgaagt ttgagattta tcagttaatt gccgaactgg
    31561 cgaagaaagg caaggggatt attattatct cctctgaaat gccagagttg ttagggataa
    31621 cagaccgtat tctggttatg agcaatggcc tcgtttccgg aattgtcgat acaaaaacaa
    31681 caacgcaaaa cgaaattcta cgtcttgcgt ctttgcacct ttaagattag gggcttccca
    31741 tgagtgcgtt aaataagaaa agttttctta cttacctgaa agagggcggt atttacgtcg
    31801 ttcttttagt tttgcttgcg attattattt tccaggcccc aacattttta agtctgttga
    31861 acttaagtaa tattctcacc cagtcatcgg tgcgtattat tatcgcgctc ggtgtggcag
    31921 ggttaattgt cacccagggg accgaccttt ctgcggggcg tcaagtaggg ctggcggcag
    31981 tggtggcagc gacattattg cagtccatgg ataacgccaa caaagtgttc ccggaaatgg
    32041 cgacgatgcc gattgcgctg gttattctga ttgtctgtgc cattggtgcg gtgatcggtt
    32101 tgatcaacgg tctgattatc gcttatctca acgtgacgcc gttcattacc acgctcggca
    32161 cgatgatcat cgtctatggc atcaactcgc tttattacga ctttgtcggg gcgtcgccaa
    32221 tttctggttt tgacagtggc ttctctacct ttgctcaggg ctttgtcgcg ctggggagtt
    32281 tccgtctctc ttacatcacc ttctacgcgt tgattgcggt ggcgttcgtc tgggtgttgt
    32341 ggaacaaaac ccgcttcggt aagaacattt ttgccattgg cggtaacccg gaagcggcaa
    32401 aagtatctgg tgtcaacgtc ggcctgaacc tgctgatgat ctacgcgttg tctggcgtgt
    32461 tctatgcctt tggcgggatg ttagaagccg gacgtatcgg ctctgccacc aacaacctcg
    32521 gctttatgta tgaactggat gctatcgcgg cgtgcgtggt aggcggtgta tcgttcagcg
    32581 gcggtgtggg gacggtgatt ggcgtggtga ccggggtaat tatttttacc gtcatcaact
    32641 atggcctgac gtacatcggc gtaaacccat actggcagta catcatcaaa ggggcgatta
    32701 ttatcttcgc cgtagcgctg gattcactga agtacgcgcg taagaagtga aatgattgtg
    32761 gtccgcattc ggtcataaac gcacataaca tcatgatctg tgtggttttg taggccggat
    32821 aaggcgttca cgccgcatcc ggcgacagga gccaaatgcc ggatgcaacg ctggcgcgtt
    32881 ttatccggcc tgcaatgagc tcaacatact gatttgtatg gttttgtagg ccggataagg
    32941 cgttcaagcc gcatccagcg acaggaacca aatgccggat gcgtctctgg ttttacaacg
    33001 ttaccgggtg ttctttctcg cctttcttaa acttcacttc cccgagctca atacaaccca
    33061 ccgggcagac gtgaccacaa agcagacaac ccacacattt ctcggtatta caatgcggtg
    33121 tgcgggtttt ctcgctccat tccatcgcct ggtgaccgcc gtcgtaacag gaaatataac
    33181 agcgtccaca gccaacacat ttatcaagat tgatacgggg atagacaata taactgcggt
    33241 ctaaatcttc cgccgggaca atattgttat tcgccagacc taccatctcc tgcaacgaat
    33301 caaatccctg atcggcgaga taatgcgata acccgctcgc catatcttct acgatgcgat
    33361 agccgtactg cataatgccg gtggtgacct gtaacgttgc tgcgccgagc aataaaaact
    33421 ccgccgcatc ctcccaggtt tcaatgccgc cgataccgct gattgggaaa tcgcgcagtt
    33481 ctggatgggt gcgcatttgc tgaatgaagc gcagggcgat cggttttacc gctttaccgg
    33541 aatatccgga aatactcgat tttccgttaa cgatcggcat accgattttc tgattaagat
    33601 cgatattggt gatggattta acggtgttaa tcgccgcaat gccatctgcg ccgccgcgct
    33661 tcgccgccag cgccacttcg cacatatcac cgatattcgg cgtcatcttc gccagcattg
    33721 gcagcgtgga accccgtttc actgcccgac aatatttttc taccagctcc gggctttgcc
    33781 cgacatcgct acccatcgca tgagaagtca tttgcggaca ggagaagtta cactcgatca
    33841 tatccgcgcc agcttcttgc accaggcgcg ccagctcctc ccattgctgc tcattttccc
    33901 ccatgatcga agcgatcaat actttgtccg ggtaatcttc cttcagccga cgcagggcgg
    33961 ccagattctc ttccaacgga tgttcagcaa tctgttccat atttttgaag ccgataaaac
    34021 cggtatcttc tttcaccaga tgatcaaaac gcggcgagac ttcgttggcg ataaaaaagc
    34081 cgatcgtttt aaacaccacg ccgccccaac ctgtgtcgta ggctttggca cacatctcat
    34141 agcagttgcc taccggcgaa gaagagaggc agaacgggtt gggaaacttc acgccgcaaa
    34201 aagtaatcga aagatctttc gttaacatga gcaagctccc tctaaatagt gatgaatcgc
    34261 ctcggcggct tctttcccgg ttttcacggc atagaccacg gttttgtcac cctcaacaat
    34321 atcgccagca gcaaagactt gcggatcgcg ggtctgataa ttttgtgttt tgatggtgtt
    34381 acgctgcggc tctaacttcg caaagtcatc cagtctggca tgctgaccga cggcgagaat
    34441 aattttatct gccgccatcg tcagttcgcc cgataaccgt acatgcttaa acgtgacttt
    34501 attgccttcc acggctactg gcgtgaatcc atcgatgatc gaaacgccca gctcccgggc
    34561 gctggcaaac tctttttcgc ttgccggaaa ctcatctaac tcttcacgcg ctacgcaagt
    34621 taccgcctga cagccgagaa ctttcagcgt gctggctacg tccatcgcga catcaccgcc
    34681 gccgataatt aatgcgcttt gtggaatgct gatatcgcct tgcgcctgtc gtgcacgtag
    34741 caggaagtcg acggcaatct caacgtcgct atgctcaaac agcggtagcc cggaaccgct
    34801 tgataacccc acggtgacca gtaccgcgcg gttttccgct ttcagttgct ccagggtgag
    34861 tgttttaccg atttcgttat tgcacttaat ggtcacgccc attttttcaa tacgggcgat
    34921 ttctgcatcc agcactgatt gcggtaaccg aaattgcgga ataccgttac gcaaccagcc
    34981 accggggtgc gcttctttct cataaatcgt gacgtcataa ccctggtttg ttagcgtcac
    35041 actggcctgc aatccggcag gacctgcgcc aataatcgcg actttgccga gcgttttagt
    35101 accgggctga taaatttcca tcccggtttg ttgttcaaaa tcagtaacaa aacgctgtaa
    35161 gcggccgata tcaatgggag catcaacacc ggcacgggta caaccgcttt ggcataattt
    35221 ctccgtcggg cacactctgg cacaaacggc accgagggca ttattttcgc gaattgtctc
    35281 ggcagcgcct ttaaaattac gaaagtagat tgagcgaata aatttccccg gatcggtctg
    35341 tgccgggcaa gcctgactac agggagcgtc gtgacataat aaacagcgag aggcttcttt
    35401 aatcgccagt aacggcgtaa aagccggagt gagttcatcc agataatttt gttgcggcat
    35461 gtgatcttcc ttaatgctat gagtaattaa ggcgaacgct aaataaatat tgcgtgctga
    35521 tattaaggct atgcgcgtgg ggaaatttaa actgtgaaag gattcacaaa tagctgcggt
    35581 tcagaatgtt atatccgcag cttattgagt gaagtaataa ttattctcaa taattaacag
    35641 aaaagagaaa atattaacga tagaaaataa gataaacaat catgccgata gataaaattc
    35701 cgaagccgat atatgccacc atataagcga aaaagagtcc gggatttttt gctcgccaga
    35761 catacaccgg ttccggtgca tttttaaccc gtttatcaat cgcgcctgcg cgccagcctt
    35821 tccatgcttc gcggaaacaa aatatcggga taaggaaaaa aacggcaacg acaaaaaact
    35881 gctgtacatc cataactact ttccttgttt cttttgtaat tcaagtaact gttcgggtgt
    35941 gacggcggga tacccctgcg catcttccgg cacctggtgc atagccggaa tagggaccag
    36001 cggccctaaa aaacgcggtt cacgtttaaa aatataaagg tcagccagcg caccgaaacg
    36061 ggcggcaaat tcacgaatac gtactgacag catatctttc ggtgacggta cggcataaca
    36121 ctgagcctga atccccatat gcagcgcaat aaataatgcc cgctcacagt ggaaacgttg
    36181 ggtgataata atgaaatcat tagtatcgaa aactttgcgt gtacgcacga tggaatccag
    36241 cgtacgaaag cctgcgtaat cgagaacaat atctgatggg tcgacaccag cagcgattaa
    36301 atctttgcgc atggtcatcg gctcattata actttgcaat gcgttatcgc cgctcagtaa
    36361 tagataattt accttaccgc tgttataggc attaatcgct ccttgaatgc ggtagcgata
    36421 atactgatta attacgccag tacgataata ttttgctgtt ccgagcacca caccgacctg
    36481 gcggtagggg agatcctgca attcgtcgta gatataaggc gcggttttcc agctcatcca
    36541 gcgatcgagg ccgagcacag tcaacagcag caagccgatc aggactaaca ggctgaggaa
    36601 cacgcgcttt aacatgcagg agactcgatc taaggaaaat ttccatcagg ctactttacc
    36661 cgctggtttt gcgcaagaaa cgacaaactg tttgccggga ttttcgacac caggagaggg
    36721 ctggcaagcc ggacaaggcg ttcacgccgc atccggcacc aggcttaagc gagaagcacg
    36781 cggtcgatat tgtgacagcc gagagctttc agtgttgcgg aggtggcatc ccactgaatc
    36841 aacggcgcat cggctttctc tgccagaact gcgcgctgga tatccgggta atcataaccc
    36901 ttgagattca atagaattaa cgctccctgc aacggtggca gagtcgggtt gaatgcggcg
    36961 ttttcagcgt agctgccgct gaaaatacga ccgtctttac attccagcgc gacaccgctt
    37021 ggcgacttac tgtaaggcat gtgcgaacgg tttgccgccg caatcgccgc ctgagaaagc
    37081 gcatcaccag tcagcgcata gccgtgatcc tgctcgtcca tcagcagcgt tttaatctcc
    37141 agatctttcg gcccaaaggc atccgccaga tagtcacgca gcgcgtgtgc ctcgcggccc
    37201 ggcagatgaa tacgcagatc cagaccgctg ttcagttcat tcataaactg acggcagtga
    37261 ccacaaggcg tgtagttaac ggtgatggct gcaagcgctt tttcaccact caaccaggcg
    37321 tggctgatcg cgctttgttc ggcatgaacg gtttgctgca ttgtcgcacc aataaattcc
    37381 atattggcac caaaatacca ggttccgctc acaccgcgcg caattgcgcc aacattaaaa
    37441 ttcgacaatg gcgtacgcgc acaggccgcc gccagcggaa gtagtgcgaa tgccagcgcg
    37501 tcttcgtcca gccccgttgc gctcttcagc gatgagactt gctccccggt caacaaagcg
    37561 gggaagtact tgtctgccag aataggttcc agtgcagatt gcaagttatc cgcaagttgg
    37621 gcaaaagcgg tttgaaaacg tggatgcatg gcgttgcctc ataatcatgt aatgggataa
    37681 cagtttacga acccgttatg gctttatatg tgatctgaat ctcattaatt atgcaacgcg
    37741 tgaaacttac atcaaaaatg cgcgacgcat cgcaaatttt agcccattac tgccagaata
    37801 atcgggaaaa ggatcggtgc gatcagcgaa gtaattatcc cgcataacac cagcgctagc
    37861 gaactaaatg caccttcctg ataatccagc tcggcgcagc gcgccgtacc gagggcgtgc
    37921 gaggcagtcc ccattgccag accgcgcgca gctttggtac gaatacgcat cgcattaagc
    37981 aatgtatggc caaataccgc gccgaggatg ccgacgaaaa tcacgcaaac tgcgctgatt
    38041 gccggaatac cgccaatact gccgccaacc gccattgcaa taggcgtggt gactgatttc
    38101 ggcaggattg acgcggcgat ttccggtgaa gcgcccatca acaatgccac ggaagtcccc
    38161 gtcaccatcg caaccacgct gccgataaaa cagatggtga tgatcgattt ccagcgcgcg
    38221 cggatctggt gtagctgctc atataaagga taggccagcg cgacgaccgc cggttgcagc
    38281 agatcgttta gcacttcgct acctttaaag taactgtcgt aagagatgcc ggtcagcatt
    38341 aaaaaaggaa tgatcaccac cattgctacc agcaacgggt taagcagggg aaacttatac
    38401 cgtgccgcca gtttgcgggc ggcaaaaaag acaatcaacg tcagcggtaa tgaccaccag
    38461 atattcgcca tcattcttct gatcctttct gacctacgac tttacgttca ccgtgtacca
    38521 gttgcgaact ccagctcacc accagaaaaa ccaccagcgt actgattgca caggaaacca
    38581 ctaccgggcc aaactgtgcg cggagcaaat caaaatattg catgacgcct acgccaatcg
    38641 gcacaaacaa tagcgccata tagcgaatca gtacgtagca ccccggattg acccatttcg
    38701 ccggaagaat ttgcaaggcc agcaggacaa acaggatcag catcccgatg atgctgcccg
    38761 gaatggttac cggtagcagg gaagcaatga aaatgcctgc atacaggcag gcataaatca
    38821 ggacgaaagc gcgtaaatat tgccagataa tgttcagtgt cttactcata atcaacaccc
    38881 cagatgagat ggattcatca tacaattaat tcgtaaaacg tgctaccgat cacattaaga
    38941 attctcagca tagttgaatt ttagggaggt aacctcgggc gagcgccccg ttcagcacac
    39001 taattctgct gtaaaaagtg cagaaactga cttaaagcat aagaaggcgt ttcgttattg
    39061 cgccagaaca caccgacgct acctttctgt tctatctttg gcaggttgag tatcaccagt
    39121 tgtccctggc tggcataacg ttgcgccagg cgtaatgaca aaatagagat catatcgctg
    39181 ctttgtaaca ggttggtact gacgttcatc gacgccgact caatcgtgtt ttctggcagc
    39241 attacgccgt ggtctaccag cgcattatca atactaatgc gaatgggggt acccgtcggc
    39301 cagacgatcc agcgccagtt ggcaagatca ttccagctga gggtggcgta tttcgccagt
    39361 gggtgctcag ggcgggcgac aaagcatact ggctcggtgt acagcacctg ataattcagt
    39421 ggcagctgca atgcgcgacc gccaattcgc ccaacgacca catcgacaga accggccaat
    39481 agatcttgca gcaatggggt catcaccttc tcttcgatat tcaggtgtag agttggcatc
    39541 ttcgtaagca aatgcaaaat cgcctgggat acgcagtcag tggcgacggg agaacagcca
    39601 attttcagac tgccgaccag tccaccttgt ttaaaacggg ccagttcatg ttgcgatcgc
    39661 tccatgtcat tgattaaccg ttgggcatgt tgcaataaca atttgccgcc ttcagtcgga
    39721 cgtaatcctt tgctgtgccg ctcaaaaagt gtgattccaa tctcatcttc gagttgtgac
    39781 aaccatttcg aaagcgcagg ttgggtgata ttcatcattc gtgcgacatg ggttagattg
    39841 ccctgttcac ccagcgccac cagcatttca aggtgatgta gttttaattt ttgcgcccag
    39901 ttcgccattt gccataacct caaggttatc tctctgcccg aaatgccatt gtacatctca
    39961 tctctttaat cacaatatcg caacatatac aaaacaaaag aaacatctac cttacctgca
    40021 ctgaggcatt tactatgact caacgacgtg atttacaagc acttatcgat gctgctccgg
    40081 tcggcaagat gcagtggcgc gttatcatct gctgttttct ggtggtaatg ctcgatggtt
    40141 ttgacaccgc ggctatcgga tttatcgccc ctgatattcg cactcactgg caactcaccg
    40201 caggcgatct cgccccgctt tttggcgcgg gtctgctagg attaaccgca ggcgcactgc
    40261 tttgtggccc gttgtccgat cgctttggtc gcaagcgggt gattgagctg tgcgtctttt
    40321 tgtttggcgc gttaagcctc gcctcggctt tctcaccgga tctgcaaacg ctggtcttcc
    40381 tgcgctttct caccggcctg ggtctgggcg gcgcgatgcc gaacaccatc accatgacct
    40441 cggagtatct gcctgcccgc cgccgcggcg cgctggtgac gctgatgttt tgcggcttta
    40501 cgctgggttc ggcgtttggc gggattgtca gtgcgcagct ggtgccggtg attggctggc
    40561 acggcattct ggtgttgggg ggcgtgttac cgctgatgct gtttgtcgca ctgctggtag
    40621 ttctgccgga atcgccacgc tggcaggtgc gccgccagct accgcaggcg gtgattgcga
    40681 aaaccgtcag cgccatcacc cgcgagcgtt atgtcgatac ccacttttat ctcattgaat
    40741 cagcctccgt tacaaaaggc agcattcgtc aactgtttat gggacgtcag ttacccatca
    40801 cgttgatgct gtgggtagtg ttctttatga gcctgctgat tatttacctg ctctcaagct
    40861 ggatgccgac gctactgaac catcgcggca tcgacctgca acacgcgtcc agggtcaccg
    40921 ccgcgttcca gattggtggt acgttaggcg cgctggcgct gggtgtgctg atggataagt
    40981 tcaatccttt ccgcgtgctg acgctgagct atgccatagg cgctatatgc atcgtgatga
    41041 ttggcttaag ccaggatgga ttatggttga tggcgctggc gattttcggc actggcattg
    41101 gcattagcgg atcgcaggtg gggctgaatg cgctgaccgc aacactttat ccaacacaaa
    41161 gtcgagccac gggcgtgagc tggtcaaatg ctatcggccg ctgcggcgcg attgttggtt
    41221 cgctgtctgg cggcgtgatg atggcgatga atttctcttt cgacaccctg tttttcatta
    41281 tcgctgtgcc tgcggcgatc agtgcggtga tgttaaccct gctgattacc gtggttcgcc
    41341 agtcgacctc tgtccctgac tcactgcctc gcgcaggcgt tgtgaacgaa taaggagcat
    41401 ctcaatgact gataacaatc aaaatagccg cgagcaattt tatcagcata tttccgggca
    41461 aaacctgacc ccgctgtggg agtcactgca ccatctggtg ccgaaaacgc ccaacgctaa
    41521 ctgtgcgcca gcgtactgga attatcagga gatccgcccg ctgctactgg agagcggcgg
    41581 cctgattggt gcgaaagagg ccgtgcgccg cgtgctggtg ctggaaaacc cggcgctgcg
    41641 cgggcagtct tccattaccg cgactttata tgccggtttg caactgatca tgccgggcga
    41701 agtggcaccg agtcatcgtc ataatcagtc ggcgctgcgt tttattgttg aaggcaaagg
    41761 ggcatttacc gccgttgatg gcgaacgcac gccaatgaat gaaggcgatt ttatcctgac
    41821 cccgcagtgg cgctggcacg atcacggtaa ccctggcgac gaaccggtta tctggctcga
    41881 tgggctggat ctgccattag tgaatattct gggctgcggt tttgccgaag attatccgga
    41941 agagcaacaa ccggtaacgc gtaaagaggg agattatctg ccgcgttacg ctgccaatat
    42001 gttgccgctg cggcatcaga cggggaactc ctcgccgatc tttaactatc gttatgaccg
    42061 cagccgcgaa gtgctgcacg atttaacccg actgggcgat gccgacgagt gggatggcta
    42121 caaaatgcgc tacgtcaacc cggtcaccgg cggctatccg atgccgtcga tgggcgcttt
    42181 cctgcaactg ctgccgaaag ggtttgcctc acgcgttgcg cgtaccactg acagcaccat
    42241 ctaccatgtg gtggaaggta gcgggcaggt catcatcggc aatgaaacct tcagctttag
    42301 tgcaaaagat atcttcgtgg tgccgacctg gcatggcgtg tcgttccaga ccacgcaaga
    42361 ttccgtttta tttagttttt cggacagacc ggtacaagaa gccctggggc tgttccgcga
    42421 agcgcgttat taatttagga gagaaaaatg actcaatacg tattcgaacc gcaagcgccc
    42481 gttaccgtac cggttgttgg cagtgatgaa caattccccg tgcgccgcgt ttactgcgtc
    42541 ggacgtaact atgccgctca cgcccgcgaa atgggctttg atccggatcg tgaaccgcca
    42601 ttcttcttct gcaaaccggc ggatgccgtt gttccggttg ccgctggtga aacgctggcg
    42661 ctgccgtatc cggcacagac cgataactac cattacgaaa ttgaactggt ggtggcgatt
    42721 ggtaaaaaag gcagcgatat cccgctggaa cacgcccatg aatatgtctg gggttatgcc
    42781 acgggtcttg atatgactcg gcgtgaccgt caaatggaaa tgcgccagat gggccgcccg
    42841 tgggaaatcg gtaaagcgtt tgatctctca gcgcccatcg cgccgctgca caaagcccgc
    42901 gatatcggcg gtattgataa tgcgccaatc tggttacagg ttaacggcga agatcatcag
    42961 cgcagcgata ttcgccatct catctggtcg gtgaatgaaa ccatctgcta tctgtcaggc
    43021 ttctttgagc tgcaaccggg cgatctgatc ttcaccggta cgccggaagg ggttggcccg
    43081 gtagtgaaag gtgatgtgat cacaggcaac gtcgagggct taacgcctat cgcggtgaag
    43141 attgtctgag gtgcatgatg aagctgtaca gtttttttaa tagttcagcg tcgtaccggg
    43201 tacgcattgc gctggcgctg aaaggcatta attaccagac cgaaggggtg aatatccgta
    43261 ttggtcagca aaatgaactg gcctatcggc ggatgaatcc ggtcgggctg gtgccgacgc
    43321 tgctgactga cgagggcgaa tcactcggac aatcgctggc gattattgac tggctggaga
    43381 ggcactaccc gcaagttccg ctggtgccgc aggaagagcc ggcccgcaac aaggtgctgg
    43441 aaattgtcta tgccatcgct tgtgatattc atccattgaa taacttgcgc gtgctgcgct
    43501 acctgaccga agaactgaac gtcagtgaag aagaaaaaaa acgttggtat gcgcactgga
    43561 tccagcaagg gttaagcgcg gtggaacaac tgttgcgcca gaaccagtcg gggcaatttt
    43621 gtgtgggcga gacgccgacg ctggcagatt gctgccttgt cccgcagtgg gcgaacgcgc
    43681 tacgcatgaa ctgcgatttg agcgggtatc cacgctgcaa agcggtgtac gacgcctgca
    43741 cacagctgcc agcgttcatt gccgcagcgc cagaaaatca acaagataaa atttcagcct
    43801 gaccaggaga actataatga cgaaagtgac acgcgcagta attgtgggag gcgggatcgg
    43861 cggtgcggca actgcgctgt cactggcccg cctggggatc aaagtggtgc tgctggaaaa
    43921 ggcgcatgag atcggtgaga ttggcgcggg catccagttg gggccaaatg cgttttcggc
    43981 gctggacagc ctcggcgtcg gcgaagttgc ccgccagcgc gcggtgttta ccgatcacat
    44041 taccatgatg gatgcggtga acgccgaaga agtggtgtgc attgaaaccg ggcaggcctt
    44101 tcgtgaccat ttcggcggcc cttacgcggt gatccaccgg gttgatattc acgcctcggt
    44161 gtgggaagcg gtactgacgc atccggacgt ggaatatcac acctccacca acgtcgtgga
    44221 tattcgcgag acggaagacg atgtcaccgt gtttgacgat cgcggcaata gctggaccgg
    44281 cgatattctg attggctgtg acggcgtgaa gtcagtggtg cgccagagct tattgggcga
    44341 tgcgccgcgt gtcaccggac atgttgtcta ccgtgcggtt atcgatcgtg ccgatatgcc
    44401 ggaagatttg cgcattaacg caccggtact gtgggcgggg ccgcattgtc acttggtgca
    44461 ttaccctttg cgcggcggca agcagtataa cctggtggta acattccaca gccgccagca
    44521 ggaagagtgg ggcgtgaagg atggtagtaa agaggaagtg ctctcttact ttgaagggat
    44581 tcccccccgt ccgcgccaga tgttggataa accgacttca tggcgacgct ggtcaaccgc
    44641 tgaccgtgaa ccggtggcga agtggggaac gaagcgcata acgcttgtgg gcgattcggc
    44701 ccatccggtg gcgcaatata tggcgcaggg agcctgtatg gcgctggaag atgcagtaac
    44761 gctgggtaag gcgctggcgc agtgtgaggg tgatgcggcg caagcctttg cgctgtatga
    44821 gtcggtacga attccgcgca ccgcgcgcat cgtctggtcg acccgtgaaa tggggcgggt
    44881 ttaccacgcc gcaggggtag aacgccaggt acgtaatctg ctgtggaaag ggaaatcgca
    44941 ggcagaattt tatcgcggca tggagtggtt gtacggctgg aaagaagata actgtttgca
    45001 accacgctaa gcacttcttc acccaatcag gcgcacgggc ggtcccctcg ccccttcggg
    45061 agagggttag ggtgagggta aaaatcttgc ccgaattaat cagtaagtca gtttgtctgc
    45121 aaacacagga ggcgctaaca tagcgcctca ttttttgcgg gtgatgatat gcgtgtgtta
    45181 ctggctccga tggagggagt gcttgactct ctggtgcgtg aattgctgac cgaagttaac
    45241 gactacgatc tgtgcatcac cgagttcgtc cgcgtggtgg atcaactgct gccggtgaaa
    45301 gtctttcatc gcatttgccc tgagctacaa aacgccagcc ggacaccatc cggtacgctg
    45361 gtgcgcgtgc agttgttggg tcagttccca caatggctgg cagagaacgc cgcccgtgcg
    45421 gtggaattag gttcctgggg cgtggatctc aattgcggct gcccgtcgaa aacggttaac
    45481 ggtagcggtg gtggggcgac gttactcaaa gatcctgaac tcatctacca gggtgcaaaa
    45541 gcgatgcgtg aagctgtacc ggcgcatttg cccgtcagcg tgaaagtgcg tctgggctgg
    45601 gacagcggtg agaagaaatt tgaaatcgcc gatgcggttc aacaggctgg tgctacggag
    45661 ctggtggtgc atggacggac aaaagagcag ggttaccgcg cggagcatat tgactggcag
    45721 gcgattggcg agattcgcca gcggctgaat attccggtga ttgccaacgg cgaaatctgg
    45781 gactggcaga gcgcgcaaga atgcatggcg atcagcggct gcgactcagt gatgattggt
    45841 cgcggggcgc tcaatattcc caacctgagc cgggtagtaa aatataacga accgcgaatg
    45901 ccgtggccgg aggtggttgc tttgctgcaa aaatataccc gtctggaaaa gcagggcgat
    45961 accgggttat atcacgtagc gcggattaaa cagtggttga gttatttgcg taaagaatac
    46021 gatgaagcaa cagaattatt tcagcatgtt cgtgtgttga ataattcccc tgatattgca
    46081 agggctattc aggcaattga tatcggtaag ctgcgataat agtggaatcg cagtttatca
    46141 gaaacaatga atcgcttatt cagcttcatc atcttgttgc gctcctttca tgagctaagc
    46201 cacatattgc cactggcgca aggagcgcgc agggggagga caatcgcctc ctccccctgc
    46261 acccccgggc tctggcgaac aaaatcgccg ctgcgcggta ccttcagctt
//
LOCUS       DBFIFM010000011        45680 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.011, whole genome shotgun sequence.
ACCESSION   DBFIFM010000011 DBFIFM010000000
VERSION     DBFIFM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.011"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(405..491)
                     /locus_tag="N7C74_000579"
     tRNA            complement(405..491)
                     /locus_tag="N7C74_000579"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(455..457),aa:Leu,seq:gag)
     gene            complement(506..838)
                     /gene="secG"
                     /locus_tag="N7C74_000580"
     CDS             complement(506..838)
                     /gene="secG"
                     /locus_tag="N7C74_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HGU2430519.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(1066..2403)
                     /gene="glmM"
                     /locus_tag="N7C74_000581"
     CDS             complement(1066..2403)
                     /gene="glmM"
                     /locus_tag="N7C74_000581"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HGU2430520.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVETALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(2396..3244)
                     /gene="folP"
                     /locus_tag="N7C74_000582"
     CDS             complement(2396..3244)
                     /gene="folP"
                     /locus_tag="N7C74_000582"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HGU2430521.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(3334..5268)
                     /gene="ftsH"
                     /locus_tag="N7C74_000583"
     CDS             complement(3334..5268)
                     /gene="ftsH"
                     /locus_tag="N7C74_000583"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HGU2430522.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNAGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(5368..5997)
                     /gene="rlmE"
                     /locus_tag="N7C74_000584"
     CDS             complement(5368..5997)
                     /gene="rlmE"
                     /locus_tag="N7C74_000584"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HGU2430523.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            6123..6416
                     /gene="yhbY"
                     /locus_tag="N7C74_000585"
     CDS             6123..6416
                     /gene="yhbY"
                     /locus_tag="N7C74_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HGU2430524.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(6572..7048)
                     /gene="greA"
                     /locus_tag="N7C74_000586"
     CDS             complement(6572..7048)
                     /gene="greA"
                     /locus_tag="N7C74_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HGU2430525.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            7296..8729
                     /gene="dacB"
                     /locus_tag="N7C74_000587"
     CDS             7296..8729
                     /gene="dacB"
                     /locus_tag="N7C74_000587"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HGU2430526.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAVAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(8769..9941)
                     /gene="cgtA"
                     /locus_tag="N7C74_000588"
     CDS             complement(8769..9941)
                     /gene="cgtA"
                     /locus_tag="N7C74_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HGU2430527.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKVEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(9957..10922)
                     /gene="yhbE"
                     /locus_tag="N7C74_000589"
     CDS             complement(9957..10922)
                     /gene="yhbE"
                     /locus_tag="N7C74_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU2430528.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLKSLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(11049..11306)
                     /gene="rpmA"
                     /locus_tag="N7C74_000590"
     CDS             complement(11049..11306)
                     /gene="rpmA"
                     /locus_tag="N7C74_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HGU2430529.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(11327..11638)
                     /gene="rplU"
                     /locus_tag="N7C74_000591"
     CDS             complement(11327..11638)
                     /gene="rplU"
                     /locus_tag="N7C74_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HGU2430530.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            11897..12868
                     /gene="ispB"
                     /locus_tag="N7C74_000592"
     CDS             11897..12868
                     /gene="ispB"
                     /locus_tag="N7C74_000592"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HGU2430531.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLKPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            13097..13375
                     /gene="sfsB"
                     /locus_tag="N7C74_000593"
     CDS             13097..13375
                     /gene="sfsB"
                     /locus_tag="N7C74_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HGU2430532.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(13423..14682)
                     /gene="murA"
                     /locus_tag="N7C74_000594"
     CDS             complement(13423..14682)
                     /gene="murA"
                     /locus_tag="N7C74_000594"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HGU2430533.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNTQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(14737..14991)
                     /gene="ibaG"
                     /locus_tag="N7C74_000595"
     CDS             complement(14737..14991)
                     /gene="ibaG"
                     /locus_tag="N7C74_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HGU2430534.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(15151..15444)
                     /gene="mlaB"
                     /locus_tag="N7C74_000596"
     CDS             complement(15151..15444)
                     /gene="mlaB"
                     /locus_tag="N7C74_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HGU2430535.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(15444..16079)
                     /gene="mlaC"
                     /locus_tag="N7C74_000597"
     CDS             complement(15444..16079)
                     /gene="mlaC"
                     /locus_tag="N7C74_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HGU2430536.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(16098..16649)
                     /gene="mlaD"
                     /locus_tag="N7C74_000598"
     CDS             complement(16098..16649)
                     /gene="mlaD"
                     /locus_tag="N7C74_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HGU2430537.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(16654..17436)
                     /gene="mlaE"
                     /locus_tag="N7C74_000599"
     CDS             complement(16654..17436)
                     /gene="mlaE"
                     /locus_tag="N7C74_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HGU2430538.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(17444..18253)
                     /gene="mlaF"
                     /locus_tag="N7C74_000600"
     CDS             complement(17444..18253)
                     /gene="mlaF"
                     /locus_tag="N7C74_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HGU2430539.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            18463..19440
                     /gene="yrbG"
                     /locus_tag="N7C74_000601"
     CDS             18463..19440
                     /gene="yrbG"
                     /locus_tag="N7C74_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HGU2430540.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            19454..20440
                     /gene="kdsD"
                     /locus_tag="N7C74_000602"
     CDS             19454..20440
                     /gene="kdsD"
                     /locus_tag="N7C74_000602"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HGU2430541.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHIPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            20461..21027
                     /gene="kdsC"
                     /locus_tag="N7C74_000603"
     CDS             20461..21027
                     /gene="kdsC"
                     /locus_tag="N7C74_000603"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HGU2430542.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            21024..21599
                     /gene="lptC"
                     /locus_tag="N7C74_000604"
     CDS             21024..21599
                     /gene="lptC"
                     /locus_tag="N7C74_000604"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HGU2430543.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            21568..22125
                     /gene="lptA"
                     /locus_tag="N7C74_000605"
     CDS             21568..22125
                     /gene="lptA"
                     /locus_tag="N7C74_000605"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HGU2430544.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            22132..22857
                     /gene="lptB"
                     /locus_tag="N7C74_000606"
     CDS             22132..22857
                     /gene="lptB"
                     /locus_tag="N7C74_000606"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HGU2430545.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            22905..24338
                     /gene="rpoN"
                     /locus_tag="N7C74_000607"
     CDS             22905..24338
                     /gene="rpoN"
                     /locus_tag="N7C74_000607"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HGU2430546.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            24361..24648
                     /gene="hpf"
                     /locus_tag="N7C74_000608"
     CDS             24361..24648
                     /gene="hpf"
                     /locus_tag="N7C74_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HGU2430547.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            24766..25257
                     /gene="ptsN"
                     /locus_tag="N7C74_000609"
     CDS             24766..25257
                     /gene="ptsN"
                     /locus_tag="N7C74_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HGU2430548.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTKGTP
                     DEA"
     gene            25303..26157
                     /gene="rapZ"
                     /locus_tag="N7C74_000610"
     CDS             25303..26157
                     /gene="rapZ"
                     /locus_tag="N7C74_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HGU2430549.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            26154..26426
                     /gene="npr"
                     /locus_tag="N7C74_000611"
     CDS             26154..26426
                     /gene="npr"
                     /locus_tag="N7C74_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HGU2430550.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            26640..27272
                     /gene="yrbL"
                     /locus_tag="N7C74_000612"
     CDS             26640..27272
                     /gene="yrbL"
                     /locus_tag="N7C74_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HGU2430551.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDVAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALASREA"
     gene            complement(27269..27997)
                     /gene="mtgA"
                     /locus_tag="N7C74_000613"
     CDS             complement(27269..27997)
                     /gene="mtgA"
                     /locus_tag="N7C74_000613"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HGU2430552.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDRVSMDQISPWMGLAVIAAEDQTFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(27994..28647)
                     /gene="elbB"
                     /locus_tag="N7C74_000614"
     CDS             complement(27994..28647)
                     /gene="elbB"
                     /locus_tag="N7C74_000614"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HGU2430553.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGRECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(28877..31213)
                     /gene="arcB"
                     /locus_tag="N7C74_000615"
     CDS             complement(28877..31213)
                     /gene="arcB"
                     /locus_tag="N7C74_000615"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HGU2430554.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVNVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDKVGEWIEEMKEEWRHDVEVLKAWVAKATKE"
     gene            complement(31309..32238)
                     /gene="yhcC"
                     /locus_tag="N7C74_000616"
     CDS             complement(31309..32238)
                     /gene="yhcC"
                     /locus_tag="N7C74_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HGU2430555.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            32912..37372
                     /gene="gltB"
                     /locus_tag="N7C74_000617"
     CDS             32912..37372
                     /gene="gltB"
                     /locus_tag="N7C74_000617"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HGU2430556.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNEI
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHNDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTET
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            37385..38803
                     /gene="gltD"
                     /locus_tag="N7C74_000618"
     CDS             37385..38803
                     /gene="gltD"
                     /locus_tag="N7C74_000618"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HGU2430557.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVQAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TREEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            38987..40036
                     /gene="yhcG"
                     /locus_tag="N7C74_000619"
     CDS             38987..40036
                     /gene="yhcG"
                     /locus_tag="N7C74_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HGU2430558.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLPWSTYVRLLSVKNADTRSFYEKETLRCGWSVRQLERQIATQFYERTL
                     LSHDKSAMLQQHAPAETHILPQQAIRDPFVLEFLELKDEYSESDFEEALINHLMDFML
                     ELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRRLRCLLIVDLKVGKFSYSDAGQMNMYL
                     NYAKEHWTLPDENPPIGLVLCAEKGVGEAHYALAGLPNTVLASEYKMQLPDEKRLADE
                     LVRTQAVLEEGYRLR"
     gene            complement(40096..40560)
                     /gene="nanQ"
                     /locus_tag="N7C74_000620"
     CDS             complement(40096..40560)
                     /gene="nanQ"
                     /locus_tag="N7C74_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HGU2430559.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLKGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQTINLKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(40557..41432)
                     /gene="nanK"
                     /locus_tag="N7C74_000621"
     CDS             complement(40557..41432)
                     /gene="nanK"
                     /locus_tag="N7C74_000621"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HGU2430560.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLGQLTDLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADAKTIFMRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(41429..42118)
                     /gene="nanE"
                     /locus_tag="N7C74_000622"
     CDS             complement(41429..42118)
                     /gene="nanE"
                     /locus_tag="N7C74_000622"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGU2430561.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLTLVKRLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(42166..43656)
                     /gene="nanT"
                     /locus_tag="N7C74_000623"
     CDS             complement(42166..43656)
                     /gene="nanT"
                     /locus_tag="N7C74_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HGU2430562.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCTAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(43765..44658)
                     /gene="nanA"
                     /locus_tag="N7C74_000624"
     CDS             complement(43765..44658)
                     /gene="nanA"
                     /locus_tag="N7C74_000624"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HGU2430563.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(44780..45571)
                     /gene="nanR"
                     /locus_tag="N7C74_000625"
     CDS             complement(44780..45571)
                     /gene="nanR"
                     /locus_tag="N7C74_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HGU2430564.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        11220 a        12440 c        11377 g        10643 t
ORIGIN      
        1 atatcagaca tgtttttcaa ctctgatcca tatcagaacg aaacactgtt agttttttct
       61 ttttaaatga tgcggccatt gcgttctcag taattaaata acattatcaa tgcgtctcta
      121 aacattacgc agaacgtagc catcagtagt tatactgaca ggagcaggat ggtttatcga
      181 gaagtaaaca tatccgtatt cgtaccggaa ctggagttga cacaataaag tgccaattat
      241 gtcagcagta gggaaaagtt tataacgaag gcgtaccggc accatcgttt caaggtacca
      301 gctacgagta aagcaactgg acgagataca gatacctgac aaccattcct cagaccagga
      361 ccaaaacgaa aaaagacgct tttcagcgtc tcttttctgg aatttggtac cgaggacggg
      421 acttgaaccc gtaagcccta ttgggcacta ccacctcaag gtagcgtgtc taccaattcc
      481 accacctcgg cacggaaact actttttagt tcgggatatc gctggtcggc ttagccggag
      541 cagctggctg agtttgttcg gttttcgccg gcgcactcag gttttcccat tcgctacctt
      601 tattggtctt gttgctattg atgttaccca gcaccagact gatgatgaag aataacgttg
      661 ccagcaacgc cgtcatgcgg gtcatgaagt taccagaacc acttgaacca aacagcgtag
      721 cggaagcgcc tgctccgaag gaggctccca tatcagcgcc tttaccttgc tgtagcatga
      781 tcagaccaac aaggccaatt gccacaataa ggaaaactac caaaagagct tcgtacataa
      841 tcaacctgtt ccttgcggag ttgccgcgta ccaatgcttc aaccaataaa gcgggagttt
      901 ataatctttc ccactgaagc gggtgtgaat actaaccaaa acgaatgacc ttcgcaaggg
      961 caattttatc gcattgtatc aactgcggaa aaaaacagca aaaccgatgt gttcgccgag
     1021 aaaaaaggcg acaatcgccg cctttttagc cagttatcta acgctttaaa cggcttttac
     1081 tgcatcggcg atgcggtgtg caaattcagt cacctgcgct tcgtcttcgc cttccaccat
     1141 cacgcgaatt aacggttcgg tgccggattt acgcaacagc acacgtccac ggctacccag
     1201 cgccgtttca acttctgcgg tcaccgcttt gactgattca tgctccagcg gatcgccgct
     1261 acctgcggtg taacgcacgt ttaccaggat ttgcgggaac attttcatgc cgctgcaaag
     1321 gtcgtgcagg ctcatatggt tacgcgccat cgccgccagc acctgcaaac cagcaacgat
     1381 gccgtcaccg gtagtggttt tatccagcag gatcacatga ccggaattct ctgcaccgat
     1441 acgccagcct ttctcctgca ttttttccag tacgtagcgg tcacccactt tcgcgcgcgc
     1501 aaatggaatt cctaactgtt tcagcgccag ttcaagcccc atgttgctca tcaatgtacc
     1561 cacagcgcca ccacgcagct ggccctgacg aagaccttca cgcgcgatga tatacatgat
     1621 ctgatcgcca tcgactttat tgccttcatg gtcaaccata atcacgcgat cgccgtcgcc
     1681 gtcgaaggca ataccgagat ccgctttttc agccagcaca cgagcctgga gcgcgcgaac
     1741 gtcggtagcc cccacttcgg cattgatgtt tacaccgttt ggctcacaac cgatagcgat
     1801 aacattcgct cccagttcgc gcagcacgtt cggcgcgatg tgataagtcg caccgtttgc
     1861 gcaatccacc acaatcttca gttcactgag gctaagttcg ttcgggaacg tggctttgca
     1921 aaactcgata tagcgacccg cagcatcaac gatacggctg gctttaccca gttctgccga
     1981 atcaacacag ctgatctctt tttccatttc cgcttcgatg gcttcttcaa ctgcatccgg
     2041 cagcttagta ccgtcaatag aaaagaattt aatgccatta tcgtagaacg ggttatgcga
     2101 tgcagatatc acaattccgg cctctgcgcg gaaggtacgc gtcagataag ccacggccgg
     2161 tgttggcatc gggccagtga agagtgcgga aaggcccgca gccgccagcc ccgcttccag
     2221 tgctgactcc agcatatagc cagaaatacg cgtgtcttta ccaataataa tcttacggga
     2281 gccgtggcgc gccagcactt tacccgcggc ccaacccagc ttaagcacaa aatcaggtgt
     2341 gatcggcgca tcccctacac gaccacgaat cccatcggta ccgaaatatt tacgattact
     2401 catagcgttt gttttccttt gcagacagag tagcttccac cactcgcatc gcttctacgg
     2461 tttctttgac gtcatgaaca cgaataatgt gcgcgccttg cattgcggca atgaccgcac
     2521 aggccagact accgctcagg cgctcggacg gccccacgtt cagcagctgc ccaatcatcg
     2581 attttcgtga catacccacc aacagcggca ggttgaaatg gtgaaattca gccaggcgcg
     2641 ccagtaatga atagttatgg gagagatttt taccgaaacc gaatccgggg tcgagcaaca
     2701 atttctcttt tgcgataccc gcctgctcgc aacgtgctat ttgctcaata aagtagcgat
     2761 tcacttctgc aaagacatcg tcatacttcg gggcttcctg catggttttt ggatttccct
     2821 gcatatgcat cagacaaacc ggtaaaccgg tttctgcagc cgcctccaga gcgccaggtt
     2881 cggaaaggga gcggatatcg ttaataatgt gagcgccaac tttcgctgac tcacggatga
     2941 cttctggttt ggatgtatcg accgagatcc agacttcgaa gcgttgagca attgcctcaa
     3001 ccacaggaat aacacgttgc aactcttctt caacgctaac ttccgccgcc cctggccgcg
     3061 tggactcgcc accaacgtca atgatcgtcg cgccagcgtt gatcatcaga ttcgcatgtt
     3121 tcaccgcatc tatcagcgag ttatgcgtgc caccatccga aaaggaatca ggcgtgacgt
     3181 tgaggatccc cattacgtga ggatggctaa ggtccagtga agtaccctgg gcaaagagtt
     3241 tcatgatgtt atccctggta tgaattgata agaaaaaacc ccggagcacg ccccggggtt
     3301 ttcggtacaa atacagtcat ctgatgcggg aacttacttg tcgcctaact gctctgacat
     3361 ggtgttaccc gggttcggcg tacgcggttc atcaaccgga cgaggagcct ttggactacc
     3421 attgtcgcca gcattgttag aagcgcctgg ttcttcccag cccgctggcg gacgtacatc
     3481 gcgacgtgcc atcaggtcat caatctgcgg tgcgtcgata gtctcatatt tcatgagagc
     3541 atctttcatc gcatgcagaa tatccatatt gtcggtcaga agctgacgcg cacgattata
     3601 gttacgctca atcagtgctt tcacttcctg gtcgatgata cgtgcagttt catcggacat
     3661 atgtttcgct ttcgctacgc tacggccgag gaacacttca ccttcttctt ccgcgtacag
     3721 cagtggaccc aatttctcag agaagcccca ctgagtcacc atgttacgtg ccaggttggt
     3781 cgcaacttta atatcgttgg acgcaccggt agaaacatgt tccggcccgt agatgatctc
     3841 ttctgccaga cgaccaccgt acagcgtaga aatctggctt tccagtttct gacggctggc
     3901 gctgattgcg tcgccctcag gcaagaagaa agtcacaccc agcgcacgac cgcgtgggat
     3961 aatcgtcact ttgtgcaccg gatcgtgctc cggcaccagg cgaccgataa tcgcatgacc
     4021 cgcttcgtgg taagccgtcg attctttctg cgcttccgtc atcaccatgg agcgacgttc
     4081 cgcacccatc atgattttgt ctttcgcttt ctcgaactca accatcgaca caacgcgttt
     4141 gttgccacga gcagcgaaca gtgccgcttc gttcaccagg ttcgccaggt cagcaccgga
     4201 gaaaccagga gtaccacggg caatgattgc cgcgtcgata tcgggtgcca atggtacgcg
     4261 acgcatgtga accttcagga tctgctcacg accgcgaaca tctggcaagc caaccacaac
     4321 ctgacggtcg aaacggccag gacgcagcag cgctgggtcg agaacgtccg gacggttagt
     4381 cgcggcgata acgatgatac cttcgttacc ttcgaagcca tccatctcaa ccagcatctg
     4441 gttcagagtc tgttcacgtt catcgtgacc accgcccaga cctgcgccac gctggcggcc
     4501 tacggcgtcg atttcatcga taaagatgat gcacggtgcc gctttcttcg cctgttcgaa
     4561 catgtcacga acacgggatg cacccacacc gacgaacatt tctacgaagt cagaaccgga
     4621 gatagtaaag aacggaactt tcgcttcgcc tgcaatcgct ttcgccagca gcgttttacc
     4681 ggtacccgga ggaccgacca tcaggacgcc tttcgggatc ttaccgccga gtttctggaa
     4741 gcggctcggc tcgcgcagat actcaaccag ttcagcaact tcttcttttg cttcgtcgca
     4801 gcccgcaacg tcagcaaagg tcgttttgat ctgatcttcc gtcagcatgc gcgctttact
     4861 cttaccaaac gacatggcac ctttgccacc gccgccctgc atttgacgca tgaagaagat
     4921 ccagacacca atcagcaaca gcatcgggaa ccaggagatg aagatagaag ccagcaggct
     4981 gggttcttca ggcggttcac cgacaacctt gacgttcttg gtcaacaggt tatccagtaa
     5041 tttcggatcc tgaaccggaa tgtaagtggt ataacggtta ctatctttct tggtaacgtt
     5101 gatttcacgt ccgttgatac gcgcttcacg aacctggtcg ttattgacct cttgtaggaa
     5161 ggtagagtaa tccaccttac ggccattaga ctcgctgggc ccaaagctct ggaatactga
     5221 catcagcaca acggcaatga ccagccagag tattaggttt ttcgccatgt cactcaaggg
     5281 attaacctct tattacaact gtgttaaaaa cagcgtcagg atactctata tccagcatct
     5341 ttcaaacttt cgtctgaaat ctcccggtta gggtttacgc ccggtcgcta caatatacac
     5401 ttcccgcgaa cgtgcacgag aagagtccgg cttacgaact ttgaccttcg taaacaggga
     5461 gcgaatttcc cttagatact catcgaaacc ttcgccctgg aacaccttca ctacaaaact
     5521 gccacctggc gctaatacat cacgacacat ttctagcgcc agttccacca gatacatggc
     5581 acgggggata tccaccgccg gtgttccgct catgtttggt gccatatcgg acatgacaac
     5641 ctggactttg ctgtcgccaa cgcgctccag cagtgctttc atcaccagtt catcacgaaa
     5701 atcgccctga agaaagtcca caccaacgat aggatccata ggtaaaagat cgcaagcgat
     5761 gatgcggcct ttgccgccaa tttgggtgac cacatattgt gaccaaccac ccggagcagc
     5821 accaaggtcg acaaccgtca ttcccggttt aaagagtttg tcactttgct gtatttcatc
     5881 aagtttaaac caggcacggg aacgtaaccc ctttttctgt gcctgttgaa catatttatc
     5941 gctaaagtgt tcctgaagcc agcggctgga gctggcagaa cgcttcttac ctgtcattta
     6001 actttcccat ggggataact catcgtaacc aattgcgtaa atttttacgc gcctatttgg
     6061 tgatatatgg gagatggcgg tagaatgacc cgttttcaat cccaacgtaa gcaaaaaata
     6121 cgatgaatct gagtactaaa caaaaacagc acctgaaagg tctggcacat ccgctcaagc
     6181 cagttgttct gcttggcagt aatggtttga ccgaaggggt gctggccgag attgaacaag
     6241 cgttagagca ccatgaactc atcaaggtga aaatcgccac cgaagatcgc gaaactaaaa
     6301 ccttgatcgt ggaagctatc gtgcgcgaaa ccggcgcctg taatgtacag gtcatcggta
     6361 aaacgctggt gctttatcgc ccaactaaag aacgtaaaat ctcgctgcca cgctaagatt
     6421 atcctaaagt tacacacatt tgctgtgtaa aacgaggggt tttccgcagg caggagagca
     6481 aaatgccacg ctctgttcgt tgataaaagg ccgctatgcg gcctttttcc tttctttaca
     6541 atacatcaac atcttgagta ttgggtaatt cttacaggta ttccacctta attacttcaa
     6601 attctacttc gccgcccggc gttttgatga ccacaacatc atcttcttct ttgccgatca
     6661 ggccacgagc aataggtgag tttacagaaa tcaggttttg tttaaagtca gcttcgtcat
     6721 cgccaacgat gcgataagtc tgttcttcgt cagaatcgag attcagcacc gttacggtag
     6781 caccaaaaat aacgcgccca ttgttgggca ttttggtgac atcaatcacc tgcgcgttcg
     6841 acagcttggc ttcgatgtct ttgatacggc cttcgcagaa gccctgctgt tcacgagctg
     6901 cgtggtattc ggcgttttct ttcaggtcgc catgctcacg cgcttccgcg atagcagcaa
     6961 tgatttcagg acggcgcaca gatttcagaa aatccagctc ttcgcgtaat ttttcagcgc
     7021 cgcgtaaggt catcggaata gcttgcattt gttatacctc ttgaatattc ctgatggggc
     7081 aagtgttcac ccaccccggc tgttcggcct gcccggcata actcctcacc gggaccagaa
     7141 gcaaaaaaat accgacccgg gtacaagtcc caggtcagct acaattcaca ttttgatagt
     7201 cattttaccc tgaagttccc gaagggtcat cgtttacttt atagggcgtt gcgccgtagt
     7261 atgacggctc gattccaggt tgttagcgcg agattatgcg attttccaga tttatcatcg
     7321 gattgaccag ctgtatagcg ttcagtgttc aggccgcaaa tgttgatgag tacattactc
     7381 aactccccgc tggtgccaac cttgccctga tggtgcaaaa agtcggcgcg tcggcccccg
     7441 ctattgatta ccacagtcag cagatggcgc tgcccgccag tacccagaaa gtgattactg
     7501 cgctggcggc gttgattcaa ctcggccccg attttcgttt caccacgacg cttgaaacca
     7561 aaggcaatgt ggaaaacggc gtacttaagg gtgacttagt ggcgcgattt ggtgccgatc
     7621 cgacgttaaa acgtcaagat attcgcaata tggtcgcgac tttgaaaaaa tctggcgtca
     7681 accaaatcga tggcaatgtg ttgatagata cctccatttt cgccagccac gataaagccc
     7741 ccggctggcc atggaatgac atgacacaat gctttagcgc tccgcctgcc gtcgccatag
     7801 ttgaccgcaa ctgtttctcc gtctcgctct acagtgcccc aaaacctggt gatatggctt
     7861 ttatacgcgt ggcatcttat taccccgtta cgatgttcag ccaggtacgc accctccccc
     7921 gtggttctgc cgaagcgcaa tactgcgaac tggatgtggt gccaggcgac ctgaaccgct
     7981 ttacgctgac gggatgcctg ccacaacgtt ctgagccgct cccgttggct tttgccgtgc
     8041 aggatggagc cagctatgcc ggtgcaattc tgaaagatga gttaaaacag gcgggtatca
     8101 cctggagcgg aacactgctg cgccagactc aggttaacga acctggaacg gtagttgcca
     8161 gtaaacagtc ggccccgctg cacgatctgc ttaagattat gctgaaaaag tcggacaaca
     8221 tgatcgccga tacggttttc cgcatgatag gccatgcgcg cttcaatgtg cctggaacat
     8281 ggcgggccgg gtcggacgct gtgcgtcaga tcctgcgcca gcaagccggt gtcgatattg
     8341 gaaacaccat tattgccgat ggttcagggc tttcgcggca taacctgatt gcccccgcca
     8401 ccatgatgca ggtgctgcaa tacattgccc aacacgacaa tgaacttaac tttatctcca
     8461 tgctgccgct agcgggctat gacggctctt tgcagtaccg tgcaggtctg catcaggcgg
     8521 gcgtggatgg aaaagtctca gcgaaaaccg gttcgttgca gggggtatat aacctggcgg
     8581 gattcattac cacagcgagc gggcaacgaa tggcgtttgt acaatatctt tctggctatg
     8641 cagtagaacc ggcggatcag cgtaatcgcc gtattccgtt agtgcgtttt gaaagccgtt
     8701 tgtataaaga tatttatcag aacaattagt caaaaagaaa ccccggcaca tggccggggt
     8761 tttgctgatt aacgcttgta aatgaactca acgccttctt cgtcgtcttc gtcccagtcg
     8821 tcatcccagt cctcatcatc ctcttcagca atctcttcaa gctgctggcg atgataatca
     8881 tcccacatga attcgacttt ctctggctgt ttcgcttctt cagcctgcac gaccgggttt
     8941 tcaatgataa aggtcatcac atcccagcag agatctttca cgcccagtcc actcgccgca
     9001 gagatcagat aatatttatc ttcccagccc agcgcctctg cgatcgcttt cgctttctct
     9061 tcggcttcta ccttatccag cagatcgatc ttgttgaaga ctaaccaacg cggtttcgcc
     9121 gccagatcct ggctgtattt ttccagctcg ctgataataa tacgcgcgtt ttcaaccgga
     9181 tcggtgccgt caatcggatc gatatcgatg aggtgcaaca ggacgcggca acgttccagg
     9241 tgcttcagga agcgaatgcc cagacctgcg ccttccgcag cgccttcaat cagtcctgga
     9301 atatcggcaa caacgaagct cttttcgttg tccattcgta ccacacccag acttggcacc
     9361 agagtggtaa acggataatc cgccactttc ggtttagccg ccgataccgc acgaataaag
     9421 gtcgatttac ccgcgtttgg catccccaac ataccgacgt cagccagcag catcagctcc
     9481 agcagcagct cacgcttatc gcccggcgta ccgttagttt tctgccgtgg tgtacggtta
     9541 actgacgatt tgaaacgggt attgcccaga ccgtgccagc cgcccttagc aaccagcaga
     9601 cgctgaccgt gtttggtcat atcgcccatg gtttcaccag taccctggtc gattacacgc
     9661 gtacctaccg gcactttaat tgtgacgtct ttaccgcgct taccggtaca gtcgcggctt
     9721 gcgccattct gaccgcgctc tgcacggaaa gatttttcaa aacgataatc gataagcgtg
     9781 ttcaggttct cgtcggcttc catccatacg tcaccaccat caccgccgtc gccgccatcc
     9841 gggccgcctt tcggaatata cttttcgcgg cggaagctca cgcaaccatt accgccatca
     9901 cctgcaacga ccagaatcga tgcttcatca acaaacttca ttttattctc cgtaaatcat
     9961 tcgcctgcgc ggggttgcga taccaccgtt gtatgcttac gtaatccgcc ccaaatacga
    10021 tgaccaatgg cggaatacat cgcgcccgca accacgacaa acgcaccgag ataacctaaa
    10081 aggtttaaca tcggtctggc gaagaaatcg ggccaggcca gtgataaaag atctgaaaaa
    10141 aacagcgtaa acagtggggt gagcgtgatg atcgcgctca cctgcgctgc ctgccagcga
    10201 gccatcgctt ccgccagggc gccatatcct accaaggtat tcagtccgca aaaaattaaa
    10261 catgcgagct gccagtggct aagctgcgct atcactccag gctttgccag agggaagagc
    10321 gcaattgtac ataaagtgta cagtaaaaac aggatctgcg gtgaggccag ccgacgcaat
    10381 aaaacctttt gcgccacgcc ataactcacc caaaccgtcg cagcaccgac cccaaagata
    10441 actccccagg tgtaatcggt tagctttgta aatatctcga ccagactggt gttaaaaaac
    10501 atcaccaggc cgctcaggag catcaatgcc cctacaacct gagtgctgcg cattttctct
    10561 ttcaggataa atacgctggc aaccatcatg ccaactggcg agagttgccc aatcacctgc
    10621 gaagcggtcg gactcaggga tttcaaggat gagctgaaca ggatgaagtt cccaaacagc
    10681 ccggcggtcg ccactgccaa caaaatcaac cagcgtggct tacgaaacac gcgtaatggc
    10741 ggcaacctct tcttcaccgc aagaatggcc cccaggccaa tactcgccat caagaaacgg
    10801 taaaacacga ttgtcggagg ttccatcacc tccagcacct gcttcattgc aattggcaac
    10861 gccccccagc aaattgctgt ggtgagcgcc aaaagaatgc caatgcctgc ctgctgcttc
    10921 ataccgtatt ccctgcaaag cgcatttgcg gattaacgtc gcaccatgcg catttttacc
    10981 ggttatcgaa tgtaaaaagc cccgcaacgt gttgcggggc tttcatccgt taccgggacg
    11041 cgaaaaactt attcagcttc gatgctgata aatttacggt ttttcgggcc tttaacttcg
    11101 aatttcactt taccgtctgc tttagcaaac agagtgtggt cacgaccgca acctacgtta
    11161 gcgccagcgt ggaatttggt accacgttga cgaacgatga tgctacccgc cagaacggat
    11221 tcgccaccga aacgcttaac gcccaggcgt ttagcttctg aatcgcgacc gttacgtgtg
    11281 gagccgccag cctttttatg tgccatttga aatctctcct caggtcttag gcgctgatgc
    11341 cagtaatttt cacatcagtg aaccactgac gatggccctg ctgcttacga tagtgtttac
    11401 gacgacgaaa cttaacgatt ttaactttct cgccacgacc gtgagcaaca acttcagctt
    11461 tgattacgcc gccatcaacg aaaggaacgc cgattttgac ttcttcaccg tttgcgatca
    11521 tcagcacttc agcgaactca acagtttcgc cagttgcgat gtccagcttt tccaggcgaa
    11581 cggtctgacc ttcgcttact cggtgttgtt taccaccact ttggaaaacc gcgtacatat
    11641 aaaactccgc ttccgcgcac accttttcaa tgattcagag tgcgctataa atattcacaa
    11701 tagggcgcga atattacgca aaacgcacgc ctttgacaag tgctacagtc aatacacgaa
    11761 gaaaaaaaac acaacttgta cggtaacgtt tatctgtgcc attttttcag tacaatcacc
    11821 ctatattcct aaccataaac cctaagttgc ctttgttcac agtaaggtaa tcggggcgaa
    11881 aagcccggct tttgcgatga atttagaaaa aatcaatgag ttaaccgcgc aagatatggc
    11941 gggtgttaat gcggcaatcc ttgagcagct taattccgac gtccaactga tcaatcagtt
    12001 aggctattac atcgtcagcg gcggcggtaa acgtattcgt ccgatgattg ctgtactggc
    12061 tgcacgagct gttggctatg agggaaatgc gcatgtcacc attgctgccc tgatcgagtt
    12121 tatccacacg gcgactctgc tgcacgacga cgttgtggat gaatcagata tgcgcagggg
    12181 taaagctacc gccaacgccg catttggcaa tgccgccagc gtgctggtag gcgattttat
    12241 ttatacccgc gcgttccaga tgatgaccag cctcggttca ctcaaagtgc tggaagtcat
    12301 gtcagaagcc gtaaacgtca tcgcagaagg tgaagttctg caactgatga atgttaacga
    12361 tccggacatc actgaagaaa actacatgcg cgttatctat agcaaaaccg cgcgtctgtt
    12421 tgaggctgcc gcgcagtgtt ccggaattct ggcgggctgt acgccggagg aggagaaagg
    12481 cctgcaggat tatgggcgct atctcggcac tgctttccag ttgatcgacg atttactcga
    12541 ttacaatgcc gatggcgaac agttaggtaa aaacgtcggc gacgatctga acgaaggtaa
    12601 accgacgctg ccgctgctgc atgcgatgca tcatggcaca ccagaacagg cacagatgat
    12661 ccgtaccgcc atcgaacagg gtaacggtcg ccatcttctg aaaccggttc tggaagcaat
    12721 gaacgcttgt ggatctcttg aatggacgcg tcagcgcgcc gaggaagaag cagacaaagc
    12781 catcgcagcg ttacaggtgc tcccggacac cccttggcga gaagcactca tcggcctcgc
    12841 gcacatcgct gttcaacgcg atcgttaatc ccctcccctc atcccgcgca gtgcgcggga
    12901 tgattccagt aaactccata agaataccta ttcagcgtaa taatgcactg aaaaaccagt
    12961 gcatacatct ttatatattc tgaatattca cactctttat aggaactttt tagagcaatc
    13021 ggccatcagg agtatagtga tgctcgacag aagaagtgtt ctgaatgaaa gcgactaact
    13081 taaggagtga ggaaagatgg aaagtaattt cattgactgg catcccgctg acatcattgc
    13141 gggtttgcgc aagaagggaa catcaatggc ggcggagtct cgcagaaatg gtttgagttc
    13201 ctcaacgctg gcgaatgcat tatcgcgccc atggccgaaa ggagagatga ttattgcgaa
    13261 agccctggga actgacccct gggttatctg gccatcacgc taccatgatc cgcagaccca
    13321 tgagtttatc gacagaacgc agttgatgcg tagctacact aaaccgaaaa aatgagtggt
    13381 ctggcggtag ccccgcgaac ggggctgcca gctctcagac gattattcgc ctttcacacg
    13441 ctcaatattt gcacctaaag cgcgcagttt gtcttcaatg cgttcgtatc cacgatcgat
    13501 gtgataaata cgatcaacca ccgtcgtccc ttccgcaata cagccagcca gcaccaggct
    13561 tgctgatgca cgcagatcgg ttgccataac ctgtgcgcca gaaagttttt caacaccgtg
    13621 acaaataacg gtattgcttt cgatttcggc gtgcgcgccc atacggctca gctctggcac
    13681 atgcataaag cggttttcaa agaccgtttc ggtgatgaat ccagtccctt ctgccaccag
    13741 gttcaacagc gtgaactggg cctgcatatc ggtcgggaat gccggatgcg gcgcggtacg
    13801 tacgttaaca gccttcggac gtttgccatg catatccagg ctaatccagt cttcgccgac
    13861 ctcgatgtcc gctccagcgt cacgcagttt cgccagcacg gcgtcgagag tatctggctg
    13921 cgtgttacgg cagataattt tgccgcgaga aatcgccgcc gccaccagga aagtaccggt
    13981 ttcgatacga tccggtagca cgcgatagac accgccgcct aaacgttcca caccttcgat
    14041 gacgatacga tcggtgccct gaccgctaat tttcgcaccc agcgtaatca ggaagttcgc
    14101 ggtatcgacg atttccggtt cacgcgctgc gttttcaata atcgtggtgc cttccgccag
    14161 ggttgcagca cacatgatgg tcaccgttgc gccaacgctg actttatcca tcacgatatg
    14221 tgcgcctttc agacgaccat cgacggaagc tttaacgtaa ccttcttcca gtttgatggt
    14281 cgcgcctaat tgttcgaggc cagaaatgtg tagatcaacc ggacgcgcac cgatcgtaca
    14341 accgccaggt agtgaaactt gcccctgacc aaagcgcgct accagcggcc ccagtgccca
    14401 gatagaagca cgcatggttt taaccagatc gtaaggtgcg cagaatacat taacgtcgcg
    14461 ggcatcaata tgcacagaac cattacgttc tactttcgca cccagctggc ttagcagctt
    14521 cattgatgta tcgacgtctt tcagtttcgg gacgttctgg atctctaccg gttcttccgc
    14581 cagtagagcg gcaaaaagga taggcagagc agcattttta gcgccggaaa ttgtgacttc
    14641 gccctggagc ttcgttggcc cctgaacacg aaatttatcc atttagtttg ttctcagtta
    14701 acaattcata tccgctaccg gcgaatcgcc catagctcaa aagccgttca gtttgcgatc
    14761 gcgcgcccac tccgcagggg tatacgcttt gatcgacaca gcatgaatgc ggttatccgc
    14821 aatatattcc atcagcggac catagaccgt ctgctgtttt ttaacccgac tcatgccgtc
    14881 aaacaactca cccacggcaa taacctgaaa gtggctgcca tcgccggaaa cgtggacttc
    14941 ctggagggag agagcgttca tcaacacgct ctgaatttca ttattttcca tgggatcttc
    15001 aatcatcagt taataaacca gcgaaacatc ttagagcaaa gttgcgctgg cataaataag
    15061 caaaaagcct cgctgataaa tcagacaagg ctcgacttgc aggcaggttt gccggacagg
    15121 cggttaacgc catatccggc ctgaaaaaat ttaacgaggc agaacatcag caggcaaatt
    15181 atacaatttc gccagggtat acactttgtc gtttaccccc tgaagcgtca cgttgttgcc
    15241 ctgctttttc gccagatcga taagatggag cagcagtgcc agtccacccg tatccacgcg
    15301 ggacacacgg ctaagatcga tgcaggtaat ccccttcacc gcttcctcac gcatttccca
    15361 aagcggtagc agaacgtcct gatccagctc tccggataac gccagtgtgt cacccgtctg
    15421 catccagctc agtgactcgc tcattatttt ttctcttcca gagtgatttt ctgttgagaa
    15481 atcgatttca gttgcgcagt caggccgtcg atacctttgg tacgtagcag cgttccccac
    15541 tcgttttgtt tggtggtgat catactgacg ccttcagcaa tcatgtcgta agcctgccag
    15601 ttgcccgtct gggagttttt acgccactgg aagtccagac gcaccggcgg gcggccattc
    15661 gggtcaataa tggtaacgcg aataggcaca atggttttat cacccagcgg ctgttctggc
    15721 gcaatctgat aggtttgacc gtgatacatc gccagcgcct gaccgtaagc ctgcttcagg
    15781 tactcacgga aagcggcaaa gtaagcatca cgttgagctg gggtcgcact cttgtaatac
    15841 tggcccagca ccagcgcacc ggcgtatttc acctgtacgt atggcagcag ttcctgatca
    15901 acaatggtac gcagataatc cgggttggcc cgaatttgcg gttgctcatt cttcaggcga
    15961 tcgaacgttt tctgcgccgc ctcgtccatc agcttatacg gattggtctg gtctgccgcg
    16021 gttgccgcac tcagcggtgc aatcaccagc aaagcgacca tcattaaacg tttaaacatg
    16081 cgtcggttct cctgaaatta tttcgttgta cccacaggtt cagtggtttc attattacct
    16141 ggcgcagcag ctggcgcatc gccactattc ttattgtcat cgcctttact accgtaaagg
    16201 aactgaccaa tgagatcttc cagcaccatc gcagacttgg tgtcctgaat tgtatcgcca
    16261 tccttcagga tagcagtccc cagttccggg tcttcaaaac cgacgtttaa tgccagatat
    16321 tgttccccca gcaggccgga agtacgaatg ctcagcgaac tggtatcagg aatatggtta
    16381 taacgttgtt caatttccag cgttacgcgc ggcagatagg ttttcgggtc cagcgtaata
    16441 tccgccaccc ggcccacaac aacgccacca atactgaccg gagagcgcgc tttcaggccg
    16501 ccaatgttat cgaacgtcgc ataaagcgtg taggtcggtt cagtacgtat tgacgtcacg
    16561 ttcgccgcct tcaggcaaac aaacagcgcc gccagcagtg ctgctaataa aaagataccc
    16621 acccaaattt catttttttt cgtttgcatg aactcaattc ccaaacatca atgcggtcag
    16681 cacaaaatcc agccccagaa cagccagaga cgagtggaca acggtgcgag tggttgcccg
    16741 gctaatcccg gcagacgtcg ggatggcgtc gtagccgtta aacaacgaaa tccacgtcac
    16801 cgtgatggcg aacaccacgc tcttaatcag acagttgacc agatccatac gccagtcgac
    16861 ggcattttgc attgccgacc agaagaaccc gctatcaatg cctttccaac tgacgccgac
    16921 cagcgatccg ccccagatcc ccacggcgac gaagataacc gtcaacagtg gtaatgaaat
    16981 aaccccagcc cagaaacggg gagaaataac ccgacgcagc ggatccaccg ccatcatctc
    17041 catactggag agttgctctg tagcgcgcat caggccgatt tctgcggtta gcgccgaacc
    17101 agcacgcccg gcaaacaaca acgcggcaac caccggcccc agttcacgca gtagcgataa
    17161 cgccaccagc atacccagac tggtttccgc actataagtg gtcagaacca gataaccttg
    17221 cagccccaac accattccga tgaacacgcc agaaactaca ataatcagca tcgacaggac
    17281 gccgacatta tagagctggc gcaccagcag cggcgcatgt ttgcgaaatt ccggtttgcc
    17341 gaccagcgca ttgaataaca ttaacccggc ccgcccaaac gttctcaggg ttttaatccc
    17401 tttatgtccg agcgacgcca gcgcatttaa cagcatgagt ggcttaactc cctggtaaaa
    17461 gatcagcgtg ataatcgccg gcaggatagc ggaacggaac cggcccgtca gctatcccgt
    17521 ccagaaactg acgtacgcgc ggatcaggat tcgcctgcaa cgcctgggca ctgccatgag
    17581 cgacaatttt tttgtccgcc aggatccagg cgtgatccgc aatacttaac acttccggca
    17641 catcgtgaga aaccaccaca caagtcacgc ccagcgcgct gttcagctca gaaatcagct
    17701 tcaccagtac gcccatggtg atgggatctt gcccaacaaa aggttcatca aacatgatga
    17761 gatccggctc cagcgcaatc gcacgcgcca gcgctgcacg ccgcgccatc ccaccggaaa
    17821 gttcagaagg cattagttta gccgctccac gcagccccac ggcctccagc ttcatcatca
    17881 ccgtactatg caacaatggc gcgggaagtt gggtatgttc gcgcagtgga taggcgacgt
    17941 tgtcaaatac gttcatatca gtgaacaacg ccccggactg aaataacatg ctcatccgtt
    18001 tgcgcactgt atacaggcgc gaacgagaca tcgccggaat attctcacca tcgaaaagga
    18061 tctcaccatg atctggtgcg atttgcccgc caatcagacg gagtagcgtc gttttaccga
    18121 tgcccgatgg ccccatgatc gccgtgatct tccctcgcgg cacggtcagg gaaatattat
    18181 cgaagatgca gcgattgcca cgcgtaaaac tgacatcgcg catatcgact aaattcgcca
    18241 cagactgctc cataattcac ccttcgtctt gcgttgattt tctaagcatg gcgctcaatt
    18301 taaccttgaa cccaacatat ttacagaata ttacccgccg tggttagcga aagctggcat
    18361 ttgttttact ttttagccgc ataaagtcaa aattaagaat ccgttacggc tttctgaaaa
    18421 tcttcagcgg accggcgagt atacctgaag aaaggacgtt agatgctttt agctacggca
    18481 ctgttaattg ttggtttact tttggtcgtt tacagtgccg accgcctggt ttttgccgcg
    18541 tctattcttt gccgaacctt tggcatcccg ccgctgatca tcggcatgac ggtggtcagt
    18601 attggtacat cgttaccaga aatcatcgtc tcgcttgccg cgtctctgca cgaacaacgc
    18661 gatttagccg ttggtacagc cctcggctca aacattatca atatattgct gatcctcggt
    18721 ctggctgcgc tggttcgtcc ttttaccgtc cattccgatg ttctacgccg tgaattaccc
    18781 ttaatgttgt tggtcagcgt ggtggccggt tccgtactct atgacggaca acttagtcgc
    18841 agcgatggta tctttctcct ctttctggct gtgctatggc tgctgttcat tgttaaactt
    18901 gcacgtcagg ctgaacgtca ggggactgac agcctgacca gagagcagct tgcagagctg
    18961 ccgcgtgacg gcggattgcc cgtcgcgttt ttatggctcg gcattgcgct tatcatcatg
    19021 ccagtggcca cgcggatggt ggttgataac gccacggtgc tggcgaatta ctttgccatc
    19081 agcgagttga cgatgggtct gacggcaatt gctatcggaa ccagcctgcc ggaactggca
    19141 accgcaatag cgggcgttcg caaaggtgaa aacgacattg ctgtcggaaa tatcattggc
    19201 gcaaacattt ttaatattgt catcgtgttg ggtttacccg cgctgataac gccaggagag
    19261 attgatccac tggcgtacag tcgtgactac agcgtgatgt tgctggtgag cattattttt
    19321 gcgttgctgt gctggcggcg ctccccgcaa ccgggccgtg gtgtaggggt attattaact
    19381 ggcggattta tcgtatggct ggcgatgttg tactggttat cgccaatact cgttgaataa
    19441 ctggaaacgc attatgtcgc acgtagagtt acaaccgggt tttgactttc agcaagcagg
    19501 taaagaagtc ctggcgattg aacgtgaatg cctggcggag cttgatcaat acatcaatca
    19561 gaatttcacg cttgcctgtg aaaagatgtt ctggtgtaaa gggaaagttg tcgtcatggg
    19621 gatggggaaa tcggggcaca tcgggcgcaa aatggccgca acgtttgcca gcaccggtac
    19681 accttcattt ttcgtccatc ctggtgaagc cgcgcatggt gatttaggca tggtcacccc
    19741 acaggatgtg gtgattgcta tctctaactc aggtgaatcc agcgaaatca ctgccttaat
    19801 tccagtgctt aagcgtcttc acataccgtt aatctgcatc accggtcgcc cggagagcag
    19861 catggcgcgc gccgcagatg tgcatctgtg tgttaaagta gcgaaagaag cctgtccgtt
    19921 agggctggca ccgaccagca gcaccaccgc cacgctggtt atgggcgatg ccctcgctgt
    19981 cgcgctgtta aaagcacgcg gctttactgc tgaagatttt gcgctctcac acccaggcgg
    20041 cgcactgggt cgtaaacttc tgctgcgcgt aaacgatatt atgcatacgg gcgatgagat
    20101 cccgcatgtt aagaaaacgg ccagtctgcg tgacgcattg ctggaagtta cccgcaaaaa
    20161 tcttggtatg actgtcattt gcgatgacaa tatgatgatt gaaggcatct ttaccgacgg
    20221 tgatttacgc cgtgtcttcg atatgggcgt ggatgttcgt cagttaagta ttgccgatgt
    20281 gatgacgccg gggggaatac gcgtgcgccc tggcattctg gccgttgagg cactgaactt
    20341 aatgcagtcc cgccatatca cctccgtgat ggttgccgat ggcgaccatt tactcggtgt
    20401 gttacatatg catgatttac tgcgtgcagg cgtagtgtaa agattcaagg ataaacaaca
    20461 atgagcaaag caggtgcgtc gcttgcgacc tgttacggcc ctgtcagcgc cgacgttata
    20521 gcaaaagcag agaacattcg tctgctaatc ctcgacgttg atggcgtact gtcagatggc
    20581 ctgatttata tgggcaataa cggcgaagag ctgaaagcgt tcaatgttcg tgacggttat
    20641 ggcattcgtt gtgcgctcac ctctgatatt gaagtcgcta tcattaccgg gcgaaaggct
    20701 aaactggtag aagatcgttg tgccacattg gggatcactc acttgtatca ggggcagtca
    20761 aacaaactga tcgcctttag cgatctgctg gaaaaattgg cgattgcccc ggaaaatgtg
    20821 gcttatgtcg gcgatgatct catcgattgg ccggtaatgg aaaaagtggg tttaagcgtc
    20881 gccgtggccg atgcgcatcc actgttgatc ccgcgcgccg attacgtgac gcgcattgct
    20941 ggcggtcgtg gcgcagtgcg agaagtttgc gacttattac tcctggcgca gggcaaactg
    21001 gatgaagcca aagggcaatc gatatgagta aagccagacg ttgggttatc attgtgctat
    21061 cactggcggt tctggtgatg atcggtatta atatggccga aaaagacgat accgcccagg
    21121 tggtcgtcaa caacaatgat cccacctata aaagcgagca tacggacacg ctcgtctata
    21181 acccagaagg ggcgctaagc tatcgattga ttgctcaaca cgttgaatat tattccgatc
    21241 aggccgtttc gtggtttacg cagccggtac ttaccacgtt tgataaggat aaaatcccga
    21301 catggtccgt aaaagcagat aaagccaagc tgaccaatga ccggatgctc tatttatatg
    21361 gacacgttga agtcaacgca cttgtgccgg actctcaact tcgcagaatc acgacggata
    21421 acgcgcagat caatctggtg acgcaggatg ttacctctga agacctcgtc acgttatacg
    21481 gaacaacatt taactccagc ggtctgaaaa tgcgcggcaa cttacgcagc aagaacgccg
    21541 agctgattga aaaggttaga acatcctatg aaattcaaaa caaacaaact cagccttaat
    21601 cttgtgcttg ccagctcact tctggccgcc agcattccgg catttgccgt aaccggagac
    21661 actgatcagc cgatacacat tgaatcggac cagcaatctc ttgatatgca aggcaacgtg
    21721 gttaccttta ccggtaatgt catcgtcacc cagggcacca taaaaattaa tgccgacaaa
    21781 gtggtcgtta cccgtccggg tggcgaacaa ggtaaagaag tgattgacgg ctacggtaaa
    21841 ccggcaacgt tctaccagat gcaggacaac ggtaaacccg ttgaaggtca cgcttcccag
    21901 atgcactacg aactggcaaa agattttgtc gttctgacgg gtaatgctta tctgcagcag
    21961 gtcgatagca acattaaggg cgataagatc acttacctgg tgaaagagca gaaaatgcag
    22021 gctttcagcg acaaaggcaa gcgtgtaacc accgttctgg tgccgtcgca gctgcaggac
    22081 aaaaacaaca aaggccagac cccggcacag aagaagggta attaattcgt tatggcaaca
    22141 ttaactgcaa agaaccttgc aaaagcctat aaaggccgtc gcgtggtaga agacgtcagc
    22201 ctgaccgtca actccgggga aattgtcggt ctgctggggc caaacggtgc cggtaagacc
    22261 accactttct acatggttgt aggcattgtg ccgcgcgatg cgggcaacat cattattgat
    22321 gatgacgata tcagtctgct acctctgcat gcacgcgcgc gccgcggtat cggctatctg
    22381 ccacaggaag cctccatttt ccgtcgcctc agcgtttacg ataacctgat ggcggtactg
    22441 caaattcgtg acgacttgtc tgctgaacag cgtgaagacc gcgcgaacga gctgatggaa
    22501 gagtttcaca ttgagcacct gcgtgacagc atggggcagt cactctccgg gggtgaacgt
    22561 cgccgtgtag aaattgcccg cgcactggct gcgaatccga aatttattct gctcgacgaa
    22621 ccgtttgccg gggttgaccc gatctcagtt atcgacatta aacgcatcat tgagcacctg
    22681 cgcgacagcg gcctgggcgt gctgatcact gaccacaacg tgcgtgaaac actggcggtt
    22741 tgtgaacgcg cttatatcgt cagtcagggg catttgatcg cccacggcac gcctacagaa
    22801 atcttacaag acgaacacgt taagcgtgta taccttgggg aagacttcag actctgatag
    22861 ggtagaagtt tgcgacgttt tagcaggaga gtacgattct gaacatgaag caaggtttgc
    22921 aactcaggct tagccaacaa ctggcgatga cgccacagct ccaacaggca attcgtctgt
    22981 tgcagttgtc gacgctggaa cttcagcagg agctacagca ggcgctggag agtaatccgc
    23041 tgcttgagca aatcgacact catgaagaaa tcgacacccg cgaaacgcaa gacagtgaaa
    23101 cgctggacac cgccgacgcg ctcgaacaaa aagagatgcc ggaagagctg ccgctcgatg
    23161 ccagttggga caccatttac accgctggta caccatccgg caccagcggt gactacattg
    23221 acgacgagct gccggtctat cagggcgaaa cgacgcagac cttgcaggat tacctgatgt
    23281 ggcaggtcga gctgacaccg ttttccgaca ctgaccgcgc tattgctacc tctatcgtcg
    23341 atgccgttga tgacaccggt tatctgactg tcccgctgga agatattctc gaaagtatgg
    23401 gcgatgaaga gatcgacatc gacgaagttg aagccgtcct taagcggatc caacggtttg
    23461 atccggtcgg tgtggcggca aaagatctgc gtgactgcct gctgatccaa ctctcccaat
    23521 tcgataaaac cacgccgtgg ctggaagagg ccagactgat cattagcgat catctcgatc
    23581 tgttagccaa tcacgacttc cgcactttaa tgcgcgtcac gcgtctgaaa gaagatgtgc
    23641 tgaaagaagc cgtcaatctg atccagtcgc tcgatccgcg ccccgggcaa tcgatccaga
    23701 ctggcgaacc tgagtatgtc attccagatg tgctggtgcg taagcataac ggtcactgga
    23761 cggtagaact caacagtgac agcattcctc gtctgcaaat caaccagcac tacgcctcga
    23821 tgtgcaataa cgcgcgcaat gatggtgaca gccagtttat ccgcagcaat ctgcaagatg
    23881 ccaaatggtt gatcaagagt ctggaaagcc gtaacgatac gctactgcgc gtgagtcgct
    23941 gtatcgttga acagcagcaa gccttctttg agcaaggcga agaatatatg aaaccgatgg
    24001 tactggccga tatcgcccag gctgtcgaaa tgcatgaatc gacgatatct cgcgtgacca
    24061 cgcaaaaata cctgcatagt ccacgaggca tttttgaact gaagtatttc ttttccagtc
    24121 acgtcaatac cgagggcggc ggcgaagctt cctccacggc gattcgtgcg ctggtgaaga
    24181 aattaatcgc ggcggaaaac ccagcgaaac cgttgagcga cagcaagtta acctctttgc
    24241 tgtcggaaca aggtatcatg gtggcacgcc gcactgttgc gaagtaccga gagtctttat
    24301 ccattccgcc gtcaaaccag cgtaaacagc tcgtttgacc caaccgataa ggaagacact
    24361 atgcagctca acattaccgg aaataacgtc gagatcaccg aggcactgcg cgaatttgtt
    24421 acagccaaat ttgccaaact tgagcaatat tttgaccgaa tcaaccaggt ctatgttgtt
    24481 ctgaaagtgg agaaagtcac ccacacctca gatgcaacac tgcatgtaaa cggtggtgaa
    24541 attcatgcca gcgcggaagg tcaggacatg tacgctgcca ttgatggctt aattgataag
    24601 ctggcacgcc agttgaccaa acataaagat aaactgaaac aacactaatt gtccgggcaa
    24661 ttagcatgtg catggcggcc tgttgtgcgg cacaacgggc catttgtacg gttaatgctc
    24721 cgagcctgtt ccactgtttg agtgggcagg ttcttaggtg aaattatgac aaataatgat
    24781 acaactctac agcttagcag tgttcttaac agggaatgta cgcgaagccg cgtccactgt
    24841 cagagcaaaa aacgcgccct ggaaatcatc agcgagctgg cggcgaaaca acttagccta
    24901 ccgcctcagg tggtttttga agctatcctg acgcgggaaa aaatgggcag taccggtatc
    24961 ggcaatggta ttgccattcc gcatggcaaa ctggaagaag atactctgcg tgccgttggc
    25021 gttttcgttc agctcgaaac gcctatagct ttcgatgcca tcgacaacca accggtggac
    25081 ttactttttg ccctgctggt gccggcagac caaactaaaa cgcacctgca tactctgtcg
    25141 ctggtggcga aacgtctggc ggacaaaacc atctgccgcc gtttacgcgc agcccagagc
    25201 gatgaagagc tgtatcaaat cattacggat accaaaggta ctccggatga agcgtagtta
    25261 ttcggtaatg tctcttttag acgttgtgag gagaaacagt acatggtact gatgatcgtc
    25321 agcggacgtt caggttcagg taaatctgtc gccctgcgtg cgctggaaga tatgggtttt
    25381 tactgcgtgg ataaccttcc cgtagtgttg ttacccgatc tggctcgaac cctggccgat
    25441 cgcgagattt ctgccgccgt cagcattgat gttcgtaata tgccggagtc accagaaata
    25501 ttcgaacagg cgatgagtaa cctgcctgac gctttctcac cgcaactact gttcctggat
    25561 gccgaccgta ataccttaat tcgtcgttac agtgacacgc gccgactgca tccgctttcc
    25621 agcaaaaacc tgtcgctgga aagtgctatc gacaaagaaa gcgatctgct ggagcctctg
    25681 cgttcgcgag cggatctgat tgtcgatacc tcagaaatgt ccgttcacga gctggcagaa
    25741 atgctgcgta cccgtctgct gggtaaacgt gaacgcgaac tgacaatggt ctttgagtct
    25801 ttcggcttca aacacggtat ccctatcgat gcagattacg tctttgacgt gcgcttcttg
    25861 ccgaacccgc actgggatcc gaaactgcgt ccaatgacag gtcttgataa gcctgtcgcc
    25921 gcgttcctcg accgccacac agaagtacac aattttatct accagacgcg aagctatctt
    25981 gagctatggt tacctatgct ggaaaccaac aaccgtagct acttgacggt cgccattggt
    26041 tgtaccggcg ggaagcaccg ttcggtgtat attgcagagc aactggcaga ctacttccgc
    26101 tcgcgcggta aaaacgtcca gtcacgccat cgtacgctgg aaaaacgtaa accatgactg
    26161 tcaagcaaac tgttgaaatc acaaacaagc tgggcatgca tgcccggcct gcaatgaagc
    26221 tgtttgaatt aatgcagggt tttgacgctg aagtgctgtt acgtaatgac gaaggcaccg
    26281 aggctgaagc caacagcgtt attgcgctgc tgatgttgga ttctgccaaa ggacggcaga
    26341 ttgaagttga agcgaccggt ccacaggaag aggaagcact ggccgccgtt atcgccctct
    26401 ttaattctgg ttttgatgaa gattaatctt catcactttg acatacaact cccttcaaac
    26461 tccccccgac aataagaaaa tcacgtactg aaatcgttct caatcaacgt caattgtaca
    26521 tttcgtgcgc tttttacttt cagaagaacc ttaagaaaac cttaagaagc attgtttagg
    26581 tttcgtttaa gttaatcgac catactggag atcgtcagaa aatatttcca ggagatggca
    26641 tgattcgctt atctgaacaa agtcccctgg gcaccggaag acatcggaaa tgttatgcgc
    26701 atccggaaga tgcccaacgc tgtatcaaga ttgtctacca tcgtggcgat ggtggcgata
    26761 aagagatccg tcgtgagtta aagtactacg cgcatcttgg tcgccggtta aaagactgga
    26821 gtggaatacc gcgctatcac ggtaccgtcg agacggattg cggaaccggt tatgtttatg
    26881 atgtgattgc tgattttgac ggcaagccct ccattacgct gaccgaattt gctgaacaat
    26941 gccgttacga agaagacgtt gcacaactgc gccaattact gaaacagctg aagcgttatt
    27001 tgcaggataa ccgtatcgtg acgatgtcgc tgaagccgca gaatatcctc tgtcaccgca
    27061 ttagtgagtc agaggttatc cccgtcgtct gcgacaacat tggtgaaagc acattgattc
    27121 cactggcgac ctggtcaaag tggtgttgtc tgcgtaagca agagcggttg tggaaacgat
    27181 ttatcgccca accggcgctg gctattgccc tgcagaaaga tctgcaacct cgtgagagca
    27241 aaacgctggc cctcgcttcg cgcgaagctt aatccagctg gtgttgttgc ataaacggct
    27301 caccgcctaa ctgatacatc tgccgtaaaa tccacgcctg acggctgcgc acgtagcccg
    27361 atggcgcgga gactttgaaa cgaagtggat taggtaatac agctgccagt aaggcagctt
    27421 ccgatcgggt aagtttgctc gcgggtttgt ggaaataacg ttgtgccgca gcttcgacgc
    27481 caaacacacc gtcgccaaat tcggcgatat tcaggtaaac cgtcaggata cgctttttgc
    27541 tccagacagt ttctatcccc agcgttaatc cggcttccag cccttttcga acccagctac
    27601 gcccatccca taaaaagaga tttttggctg tctgttgaga aatcgttgaa gcaccgcgaa
    27661 tacggttttc attgcgctca ttgtgcgcca gggctttctc aatggaagcg acatcaaagc
    27721 cccagtgctc aggaaatgtc tgatcttctg cggcaatcac cgccagtccc atccacggcg
    27781 agatttgatc catactgacc ctgtcagaat gtgccacgta acgaaaatta ccatgcagcc
    27841 aggcgctgac ctgtcgctcg accattaccg cagagaaggg aacaggtgca acgctaaaca
    27901 acgcgatgcc cccgccccag aaaacggcga ggacaaccat taaccgcaga agaaaacggc
    27961 gaacgaaact aaacaccgtt aagcggcttt tactcattca gccagaacca gcacgcggga
    28021 aaccagctta tcaatgccgc tcgccgcttc tgcaatgttc tgcgccagca tatatgccgg
    28081 ggtggtgaca atcttattgt cttcatcaac cacgatatca tcgacaggac acggcacatg
    28141 ctccgcgccc atctcttcca gcacttctgc ggtatcgata tcagtaccga tggtcaaacg
    28201 cagcgggaaa tcgaaaattt tcggcagcat cgccggggca atacacataa aaccaagcgg
    28261 ttttccggcc tgatgcatcg cttgtgccag cgcctttaat tcacggtcaa cggtgcattc
    28321 gcgaccaaga ctggcaaaat tgcttaaatt ctttgccgcg ccaaaccccc ccggcacaat
    28381 caacgcatcc agttcagcgg catcggcctg ggccagagga cggatttcac cgcgcgttat
    28441 tcgtgccgct tcaatcagca cattgcgcgt ttccgtcatc gcttcgccag ttaaatgatt
    28501 gataacatca acctgctgct tatccggtgc aaagcagacc gcctgcgcac cgctgcgtga
    28561 aatagctaac aacgtcaaca ccgcttcatg aatttcagaa ccgtcataga cgccgcatcc
    28621 gctcagaatt acgccaattt tcttcattat gatcatcctt tcgcaattga ctgaaacaca
    28681 ttaatttttt taataaaaat ggtacgcatc acacatttaa ctgattcatg taacaaatca
    28741 tttaagtttt gctatcttaa ctgcgtgcgg cctgaaaaac agtgcagtgc ccttgtaact
    28801 catcataata atttacggcg cagccaagat ttccctggtg ttggcgcagt attcgcgcac
    28861 cccggtctag ccggggtcat tctttagtgg cttttgccac ccacgctttc agcacttcta
    28921 cgtcgtgacg ccactcttct ttcatctctt caatccattc accgacttta tcttcccagg
    28981 cgggaaggtc aggagactga atttgctgac ccagctgttg cagatggcgc aaccccactg
    29041 accccgccgc acctttaatt ttatgtcctt cctcaacaat gcctttttta tcctgcgccg
    29101 tcagattcga ctccagcacg ttgacatagc ctggcatcat cttctcaaac accgctaacc
    29161 cgtcggtgat cagcttcggt cctacaagtt cgagatactg ttccagcatg ggaatatcga
    29221 gcaatgcttc tgatttactg ttctcttctg tcgtcaccgt actctcctca tcatcctggg
    29281 tatcccagaa tttcttgatc atcgcggtta gcgccggaac agaaagcggc ttactcagca
    29341 catcatccat tccagcattg aggtactctt gtttgtcttt cagcacgtta gcggttaagg
    29401 ccaccagcgg cggtaaatcc tctcgcggat aacgcttcgt cagttcacga gagatatcca
    29461 gcccggtcat atctggcaac tgaatatcca gcaacaccag gtcgtattcg cccggtttaa
    29521 acatctccag cgccgccttg ccggtcatgg cgacatcaac gctgttacct aatttttcca
    29581 gcacagaacg cgcgacaatc acgttcagtt caatgtcttc caccagcagc acattcagcg
    29641 ccggtaaagg catatcgtct tcatcaaacg catcatcgac ctcttctgct accgacggtg
    29701 cgtggatcgt caacgtaaag gttgaacctt taccctgttc gctggtaacc gtaatatcgc
    29761 cgcccatatt tttcgccaga cgacgagaaa cggccagacc aataccggtg ccggtggcag
    29821 gtttaccgcc atgactgtct ttcacctggt aatacatggc gaagatttta tccagctcat
    29881 cctgcggaat gccgatacca gaatcttcca cttcaaaatg cagcatatcg ccttcatcgt
    29941 agcgcacgcg cacggtaacc tggccttgct gggtgaattt gacggcgtta ctgatgaggt
    30001 tccacaggat ctgccgtaaa cgcgtcccgt cggtaatgac ctgatgcggt aatggcagcg
    30061 tcggctccag gttaaagcgc aatccttttt gttgcgcctg caatgcggag agattttcca
    30121 gatcggcaag gaagctggtg aaatcaaccg gttgattatc aagctggacc ttgcgccgtt
    30181 ccatcttatc catgtcgata atatcgttaa agatattccc cagcgtgacg gccgaaacat
    30241 ggatggtctt gagatatttt tcctgctcgg cggtgagttc ggtatccagc agaatgcggc
    30301 tcaggccgac gataccgttc agcggtgtac gcaattcgtg gctgatggtg gaaataaacg
    30361 tcgttttgtc gcggctggcg cgttcaagcg catcctgata ccgcttacgc tcggtaatgt
    30421 cgcgaccaaa gcccatcaaa ccgtgacgtt tacccacgcg gtcgtagtac ggcactttac
    30481 gaatttcaaa gcaggctttg cgtccgtcgg gataatccag ccactgctca taggtcagtg
    30541 acacattatg acggaacact ttttcatcgg tttcaatgac tttcgcagcg gcttccggtg
    30601 agtaaacatc agcaggtttc aggtgaacca gttgtttttc gctttttccg gtcagcagct
    30661 ccatcgcgcg gttacagccg gaaaactctt tatcttcgtt acgataaaaa accaggtcgg
    30721 gtgaagcatc aaggaaggaa cgtaaaaatg aggattgctg ctcgagctga atttgtgtct
    30781 cttcgcgctc tttgatttca attttcagtt ggccgaaggt ttcctgcagt tctgcttccg
    30841 ctttttcacg aacggcaatt tcctgattta gctgggcaat attatctttt aactgaacgt
    30901 tgaggctcaa atcgcgctcg cgcatctcct ccagtttttg caccagccgt gacagacgtt
    30961 gtcgtgactc ctccagttgc tcgacgacca ccgatagaaa gtagaccgcc cacggcgtaa
    31021 tcagcaaacc aaagaagata gaacgaataa catcaatgct ttcgacctga ccatgcagca
    31081 ccatggttac cgccatttgt accacaatgg caagaacgac gagggccagc gccagcaaca
    31141 ttgagaagcg caccagacct aacttcatca tcaggtcaac ataatactgc gccagcagac
    31201 gaatttgctt cattagggaa ttccttcacg acaacctgcc ccaataatac ccaattctga
    31261 cagttacgtt ataaattgtg tgagaaatgc ggaattcccc tcaacgacct actccgttgg
    31321 aggtagccac ggacgtccca gcgccgatcc ctgtacgcca tgttcattca gatagcgatc
    31381 cagctcgacc attcccgtcc atcgattttc gcaccacaga ggagcaagca gcgtcgggcg
    31441 acgggcgctg gcggaaatgc ggtggtagat cacttccggt ggcgtatggc gaatcatttc
    31501 tccggcagtg agcgtgtaat cctccagttc aataccgttc aaacgccccg cttcccaggc
    31561 tttcgccata atgctgcctt tcacaatatg cagcggatgc agctttatgc cgtccacgcc
    31621 ggtttcaacc actcgctcca gcgtttgcaa acattcagcc tgcccttcgc caggcaggcc
    31681 gacaattaag tgagaacaca ccttcagccc gcgctgacgt gccagctggg ttgtacgctg
    31741 ataacaggca aaatcatggc cgcgattaat gcgatgcagg gttttgtcgt gggcggtttg
    31801 tagccccagc tccagccaca cttcgtagcc ctggtcctta tattcgcaaa gcagatccag
    31861 caccgcatcc ggcacgcagt ccgggcgagt accaacacac aaaccgacaa tattggcctg
    31921 gctcaccgcc tgctgataca tagaacgcag cacctgaact tccgcaaagg tgctggtata
    31981 cgcctgaaag taggccagat agcgtttagc gcggttaact aaattcgcct ggtgcgccag
    32041 ttgctcggca atggaacgat gttgctgcgc ttcatcggca aatgaggcaa cattacagaa
    32101 tgtgcagccg ccacgcccga tggtaccgtc acggttaggg cagctaaaac cgccgtgcag
    32161 cgtcagctta tgcacctttt gcccataacg acgggtgaga tcaccaccaa acatattgac
    32221 taatttctgt aactgcataa tctgatagac cgcgccttga aaagaggcca aagcctgcca
    32281 tttttagctc aattcggcga tgacttggat caatcgtccc ggcctgcttt tatcaactgc
    32341 ataaccaatc aaaattaccg aaatttcatg cataatcaca taaatcactt ttgcttatct
    32401 tgtgtcagat ttttttatct ccttatggat tttaggcaaa acagtagcat gaaacgtcat
    32461 taccaattaa ggcagtataa aatgctggtt ttctcgtcag ttcaaggcag gataagggtt
    32521 aacacgcctt tatgacagtc aggaattggc tgtttctcta acgacgcccc ttttagcctt
    32581 aattagaaaa tccattttaa tttcagtcaa ttaataaaga attttgcgct aaagcacatt
    32641 tctgtaccaa taagcttgcc atttgacctg tatcagcttt cccgataagt tggaaatccg
    32701 ctggaagctt tctggatgag cagcctgctc atcatattta tgcagtaatt gagatcccct
    32761 cttcaccgta ttaaccgatg cgaaaaggac aacaaggggg cgaatgcgag gcgcgcgtat
    32821 gacacgcaaa ccccgtcgcc acgctctttc tgtgcccgtg cgcagcggtt cggaagtggg
    32881 gttcccgcag agcctggggg aggttcacga tatgttgtac gataaatccc ttgagaggga
    32941 taactgtggt ttcggcctga tcgcccacat agaaggcgaa cctagccaca aggtagtgcg
    33001 tactgcaata cacgcactgg cccgcatgca gcaccgtggc gcgattctcg ccgatggtaa
    33061 aaccggtgac ggttgcggct tgctgttaca aaaaccggat cgcttttttc gcatcgttgc
    33121 gcaggagcgc ggctggcgtt tagcgaaaaa ctacgccgtc gggatgctct tcctgaataa
    33181 agatcctgaa ctcgccgctg ccgcacgccg catcgttgaa gaagagctgc aacgcgaaac
    33241 cttgtcgatt gtgggctggc gtgatgtccc cactaacgaa ggcgtgctgg gtgaaatcgc
    33301 cctctcctct ctgccacgca ttgagcaaat ttttgtgaac gccccggcag gctggcgtcc
    33361 gcgcgatatg gagcgccgtc tgtttatcgc ccgccgccgc attgaaaagc gtctcgaagc
    33421 cgacaaagac ttctacgtct gtagcctgtc gaatctggtg aacatctata aaggtctgtg
    33481 tatgccggcg gatctgccgc gcttttatct agatcttgcg gacctgcgtc tggaatcggc
    33541 catttgcctg ttccaccagc gcttctccac taacaccgta ccgcgctggc cgctggcgca
    33601 accgttccgc tacctggcgc ataacggtga aatcaacacc atcaccggta accgccaatg
    33661 ggcgcgtgcg cgtacttata aattccagac accgcttatc cctgacctgc acgacgccgc
    33721 accgttcgtc aacgaaatcg gctctgactc cagttcgatg gataacatgc tggaactgct
    33781 gctggcaggc gggatggata tcatccgcgc catgcgtcta ttagtaccac ccgcctggca
    33841 gaacaacccg gatatggacc cggaactgcg tgccttcttt gactttaact ccatgcatat
    33901 ggagccgtgg gatggcccgg cgggcatcgt gatgtctgac ggtcgttttg ctgcctgtaa
    33961 cctcgaccgt aacggtctgc gtccggcgcg ctacgtcatc accaaagata agctcatcac
    34021 ctgcgcctct gaagtcggta tctgggatta ccagcctgac gaagtggtcg aaaaaggccg
    34081 cgtcgggcca ggcgaactga tggtaatcga cactcgcagt gggcgtattc tgcactcggc
    34141 agaaaccgat gacgatctga aaagccgcca cccttataaa gagtggatgg agaaaaacgt
    34201 ccgccgactg gtaccgtttg aagatctgcc cgatgaagaa gtgggtagcc gcgaactgga
    34261 cgacgacacg cttgccagct accagaaaca gtttaactac agcgcggaag agctggactc
    34321 cgtaattcgc gtactgggcg aaaacggtca ggaagcggtc ggttcgatgg gcgatgatac
    34381 cccattcgcc gtgctctcca gtcagccgcg cattatttac gactacttcc gccagcagtt
    34441 tgcccaggtg actaacccgc caatcgaccc gctgcgtgaa gcgcatgtta tgtcgctcgc
    34501 caccagtatc ggtcgtgaaa tgaacgtctt ttgcgaagca gagggccagg cgcaccgttt
    34561 aagctttaaa tcgccgattc tgctctactc cgatttcaaa cagctcacga cgatgaaaga
    34621 ggagcactac cgcgcagata cgctggatat cacctttgac gtcaccaaaa ccacgctcga
    34681 agcgacggtc aaagagctgt gcgacaaagc cgaaaaaatg gtacgtagcg gcaccgtgct
    34741 gctggtgctc tccgaccgga atatcgctaa agatcgcctg ccggttccgg ctccaatggc
    34801 ggttggcgcg atccagaccc gtctggtcga tcaaagcctg cgttgcgatg ccaacatcat
    34861 cgtcgaaacc gccagcgccc gcgatccgca ccacttcgcc gtgttgctgg gtttcggcgc
    34921 gacggctatt tatccgtacc ttgcctatga aacgctgggc cgcctggtag acacccacgc
    34981 gattgccaaa gattatcgta ccgtgatgct caactaccgt aacggcatca acaaaggctt
    35041 gtacaaaatc atgtccaaaa tgggcatctc caccatcgcc tcttaccgct gctcgaaact
    35101 gtttgaagcg gtcggtctgc acaatgatgt agtgggcctg tgcttccagg gggcggtcag
    35161 ccgcattggg ggggcgagct ttgaagactt ccagcaggat ctgctgaatc tctcgaaacg
    35221 agcctggctg gcgcgcaagc ccatcagcca gggcggcctg ctgaaatacg tccacggcgg
    35281 cgaataccac gcctacaacc cggacgtggt gcgcacgctg caacaggcgg tacagagcgg
    35341 cgagtatagc gactatcagg aatacgcgaa gctggttaat gagcgtccgg caaccacgct
    35401 gcgcgatctg ctggcaatta cgccgggtga aaacgcggtc aacattgccg atgttgaacc
    35461 ggcaagcgaa ctgtttaaac gcttcgatac cgcggcgatg tctatcggcg cgttaagccc
    35521 ggaagcccac gaggcgctgg cggaagcgat gaacagcatc ggcggaaact cgaactccgg
    35581 tgaaggtggt gaagacccgg cgcgctacgg caccaacaaa gtgtcgcgca tcaagcaggt
    35641 ggcttccggt cgctttgggg ttactccggc gtatctggtc aatgccgacg tcattcagat
    35701 taaagtcgcc cagggcgcga agccgggcga aggcggtcag ttgccgggcg acaaagtcac
    35761 tccttacatt gccaaactgc gctattcggt gccgggcgtg acgctgatct ccccgccgcc
    35821 gcaccacgat atctactcta tcgaagactt agcgcagctc attttcgacc tcaagcaggt
    35881 taacccgaaa gcgatgatct ccgtgaagct ggtttccgaa ccgggcgttg gcaccatcgc
    35941 caccggtgtg gcgaaagctt atgccgattt aatcaccatc gcaggctatg acggcggcac
    36001 cggcgcaagt ccgctttcat cggtgaaata cgcaggctgt ccgtgggagc tgggacttgt
    36061 tgaaacacaa caggcgctgg ttgccaacgg cttgcgtcat aaaatccgtt tgcaggtcga
    36121 tggcggcctg aaaacgggtg tcgatatcat caaagcggcg attctcggcg cagaaagctt
    36181 cggcttcggc actggcccaa tggtcgcgct cggctgtaaa tatctgcgta tttgccacct
    36241 gaacaactgc gcaacgggtg tggcaactca ggatgacaaa ctgcgtaaga atcactatca
    36301 cggcctgccg ttcaaggtga cgaattactt tgagtttatc gcccgtgaaa cccgcgagct
    36361 gatggcacag cttggcgtaa cacgtctggt ggatctgatt ggtcgcaccg acctgctgaa
    36421 agagctggac ggtttcaccg ccaaacagca gaaactggcg ctgtcgaagc tgctggagac
    36481 cgccgaaccg catccaggta aggcactcta ctgcaccgaa accaacccgc cgtttgataa
    36541 cggcctgctg aacgcgcagt tgctgcaaca ggcgaaaccg tttgtcgatg agcgccagag
    36601 caaaaccttc tggttcgata tccgcaacac cgaccgctct gtcggcgcgt cgctttcagg
    36661 ctatatcgcc cagacgcacg gcgatcaggg gctggcagcc gatccgatta aagcgtactt
    36721 caacggcacc gcaggccaga gcttcggcgt gtggaacgcg ggcggcgtgg aactgtacct
    36781 gaccggtgat gccaacgact atgtcggtaa aggcatggcg ggcggcttaa tcgccattcg
    36841 tcctccggtt ggttccgcct tccgcagcca tgaagcaagt attatcggca acacctgcct
    36901 gtatggagcg acgggtggtc gtctgtatgc cgcaggccgc gcgggtgaac gtttcggcgt
    36961 gcgtaactcc ggtgcaatca ccgtggtaga aggcattggc gacaacggct gtgaatatat
    37021 gacgggcggt atcgtctgta ttctgggtaa aaccggcgtt aacttcggtg cgggtatgac
    37081 cggcggcttc gcttacgttc tcgatgaaag cggcgatttc cgcaaacgcg ttaacccgga
    37141 actggtggaa gtattaagcg ttgacgattt ggcgatccat gaagagcatc tgcgtggtct
    37201 tatcaccgag catgtgcagc ataccggttc ccagcgtggt gaagagattc tggcgaactg
    37261 gtcaaccttc gccactaaat ttgcgctggt taaaccgaag tccagtgatg tcaaagcact
    37321 gctgggtcat cgtagtcgta gcgcagcaga gttgcgcgtg caggcgcagt aaggggtagc
    37381 aacaatgagt cagaatgttt atcaatttat cgacctgcag cgcgttgatc cgccaaagaa
    37441 accgctgaag atccgcaaaa ttgagtttgt tgaaatttac gagccgtttt ccgaaggcca
    37501 ggccaaagcg caggctgacc gctgcctgtc gtgcggcaac ccatactgcg agtggaaatg
    37561 cccggtacac aactacatcc cgaactggct gaagctcgcc aacgaggggc gtatttttga
    37621 agcggcagaa ctgtcgcacc agaccaacac tctgccggaa gtatgcggac gtgtctgccc
    37681 gcaagaccgt ctgtgcgaag gttcctgcac cctgaacgat gagtttggcg cggtgaccat
    37741 cggcaacatt gagcgttata tcaatgataa agcgttcgaa atgggctggc gtccggatat
    37801 gtccggcgtg aaacagaccg gtaaaaaagt ggcgattatc ggcgcaggtc cggcaggtct
    37861 ggcgtgtgca gatgtcctga cgcgcaacgg cgtacaagcg gtcgtcttcg accgtcaccc
    37921 ggaaattggc ggcttgctga ccttcggtat tccggctttc aaactggaaa aagaggtaat
    37981 gacgcgccgc cgtgaaatct tcaccggcat gggtattgaa ttcaaactca ataccgaagt
    38041 gggccgcgac gtacagctgg acgatctgct gagtgattac gatgccgtgt tccttggcgt
    38101 cgggacttat cagtcaatgc gcggcgggct ggaaaacgaa gacgccgatg gcgtgtacgc
    38161 agcgctgccg ttccttatcg ccaacaccaa acagttaatg ggctttggcg aaactcgtga
    38221 agaaccgttc gtcagcatgg aaggcaaacg cgtggtagta cttggcggtg gcgataccgc
    38281 aatggactgc gtgcgcacgt ctgtacgcca gggggcaaag cacgttacct gtgcctatcg
    38341 tcgtgatgaa gagaacatgc cgggttcccg ccgcgaagtg aaaaacgcgc gggaagaagg
    38401 tgtagagttc aaattcaatg tccagccact gggaattgaa gtgaacggta acggcaaagt
    38461 cagcggcgta aaaatggtgc gtactgaaat gggcgaaccg gatgccaaag gccgtcgccg
    38521 cgcggagatc gtggcaggtt ccgaacatat cgttccagca gatgcggtga tcatggcgtt
    38581 tggtttccgt ccacacagca tggaatggct ggcaaaacac agcgtcgagc tggattcgca
    38641 aggccgcatc atcgccccgg aaggcagcga caacgctttc cagaccagca acccgaaaat
    38701 ctttgctggc ggcgatatcg tccgtggttc cgatctggtc gttaccgcta ttgccgaagg
    38761 tcgtaaggcg gcagacggca ttatgaactg gctggaagtt taagctctgt cccagcgccg
    38821 cctcctcaga aaagagtcgg cgctgaaatt ctcctttcgt tctggaaagc gcctcgcata
    38881 atctgaagaa aaccaatgta atacgcacat ctggattgtt accggcagat gcccggcgta
    38941 tagctgattc cattattcaa cactgcgcca tataaaggga aagataatgg aatcgctctc
    39001 agagagaacc tcaacaggct accagcaaat ccacgacggt attattcacc tggtcgatag
    39061 cgcccggacg gaaacggtac gtagcgttaa cgcgctgatg accgcgacat actgggaaat
    39121 tggccgacga attgtcgaat ttgaacaagg tggcgaggcc agggctgcgt atggtgcgca
    39181 gctaatcaag cgactatcaa aggatttaag tctaaggtat aagcgtgggt tctctgcaaa
    39241 aaacttacgc caaatgaggc ttttttacct cttttttcaa catgttgaaa ttcgccagac
    39301 agtgtctggc gaattaacac cattgccctg gtccacttac gtccgtttac tttccgttaa
    39361 aaacgctgac acccgcagct tttatgaaaa agagacgctc cgctgtggct ggtctgttcg
    39421 ccagctagaa cggcaaattg cgacccaatt ttatgagcga acactgctgt cacatgacaa
    39481 atcagccatg ctgcaacaac acgctcctgc cgagacgcat attcttccgc aacaggcgat
    39541 acgcgatccc tttgtgctcg aatttctgga attgaaagat gaatactcag aatccgattt
    39601 tgaggaggcg ctgatcaacc acctgatgga tttcatgctg gaacttgggg atgattttgc
    39661 ctttgttggt cggcagcgaa ggttacgcat tgatgacaac tggtttcgtg tcgatctgct
    39721 gtttttccac cgccgtttac gctgcctgct aatcgtcgat ctaaaagtgg gcaaattcag
    39781 ctatagcgat gccggacaga tgaatatgta tctcaactac gccaaagagc actggacgct
    39841 accggatgaa aatccgccca tcggtctggt tctctgtgca gagaaaggag tcggagaagc
    39901 gcattatgca ctggctggtt tgcctaacac cgttctggcg agtgaatata agatgcaact
    39961 acctgatgaa aaacgactcg cggatgaact cgttcgaaca caggcggtgc tagaagaagg
    40021 ctatagactc cgttaatttc agcggtttcg aattctgcag acactgtctg cagaatcttt
    40081 aaaacgaact aagttttaag ccattaaatc agccttaacc ttcaccacca cctttttaat
    40141 ctcaccaggt tcgccgacaa cgcatcctgg tttatgcggt tctcccggca taaacacggc
    40201 gaacattccc ggttttaagt tgatggtttg ctcgttttca atggcgctgc aaagctggta
    40261 atcatcttca tggtggaact cttcacactg gcgcgcagtg cctgccgtgc caaacagaat
    40321 ccgttcctca ccttttaata acagctggat atcaatgtat tgctcgtgca attccgcttt
    40381 tttctcgacg ggcgattgag tgttaaacgt catgacattc ataaaaatat tgtcgccctg
    40441 taattcgtaa cgacccggcg ctttttcttg cggtctggca gctaatgcca gcgttaacgc
    40501 gtcctgtaac gcaggatgta acccagcaga cggtaatgac tgtacttcac ccatcatcat
    40561 aatttttctc cctgggccaa cagcgcagcc ccaagtaaac ctgcatcatg gcggtagtgc
    40621 gccgccagta aatcaacatg aaatgccgct ggctcctgcg ccagatacgt ttccaccagc
    40681 gccagatacc cttctgccag accaacgctg ccaccgacca ccacgcactg acaatcagtt
    40741 gtggctttaa tatcagcgat cagccttgcc agcacatgtg cggagcggtg aatcagctgc
    40801 tgcgcctgct cgtcgccctg cccggcgcgc atgaaaatag ttttcgcatc cgcgccagcc
    40861 aactccccct gcgctgccgc tgcaataccg cgaccagagg caatcgcttc cacgcaacct
    40921 gtgcgtccac agccgcagac tgggccgtgt ggatcggcaa gcgtatgccc gatgtgcccc
    40981 gccagaccgc cagggccggt aagcagtttg ccgccgctca ctacaccgcc gccgacgcca
    41041 gtggaaacgg tgataaacac catatcggtt atatcgcctt ccagcgcctg atactctgcc
    41101 catgctgcgg cctgggcgtc gttaatggca atggtcggca aatcggtaag ttgccccagc
    41161 gttttgacta acggaaagtg tagcaatcca ccaagattat gcggattcag cgccagcaaa
    41221 ctgccgtcgc gaataattcc ggttgaagcg atggcaaccc gctgcgcatg agcttgcaac
    41281 ggagagacta atgcggataa ggcatcacgc aaggcttctg gtgtctggct ggctggcgta
    41341 ggaagttcac gacgatcgcg gatctgcccg tcagcgccaa tcagcgcggc ggcaagttta
    41401 gtaccgccga tatcaatcgc cagtgtggtc atagcaccgc ctttttcatc gctgtgttgt
    41461 accactgaca aatgtgttca agacgcgtaa tggcagaacc gaccgtcacc gcccacgcgc
    41521 cgtggcgcat cgcatcagcc gcctgagcag gcgtgttgta acgcccttcg gcaatcaccc
    41581 gacatccggc gtcgctcaac cttttcacca gcgtcagatc cggctcttct ggcgtttcag
    41641 gcgtggtata gccagaaagc gtagtgccaa taatttcggc tcccagcttt tggcatgcca
    41701 ggccgtcttc cggcgttgag cagtcggtca tcgccagtaa accgtgatga tgaatacgtg
    41761 ccagcagcgt ttcaacaggc accggacgcg ggcggtcggt gccgtcaatg gcgataatgt
    41821 ccgcgcccgc ctgcgccagc gcatcgacat cttcaatata ggccgtgatg cgtaccggag
    41881 aatcctccag atcgcgtttc acaattccaa taatcggcac gctcaccacc gcacgcgtgg
    41941 cttgcagatt tgccacacct tcgatgcgaa tggcaaccgc gcccgcctgc tctgccgcta
    42001 atgccatggc tgcgacgatt tcgggtttat cgagcgggct gtccggaacc ggctggcagg
    42061 agacaatcag gccaccgtta gcagcgattt tttgatccag ttgtgcaagt aacgacatac
    42121 atcttccctt agcgaaaggc ccgatacaga gaccgggcaa caggattaac ttttggtttt
    42181 gactaaatcg tttttggcgc tgccaaacgg cacggcaccg ctaaatggtt tgccgtcgat
    42241 agcgtcatga gtacgcaacg cttccgggcg cagccaacgc tgaacacgag aaggcatatc
    42301 cagaccaatc agcaggatca ccacaaatgt cagactgaac gagagcgatg ccagtgcagt
    42361 acccagatcc agacgttgag cgatcaacgc gccgatgatt ggggccagtg cgccacccaa
    42421 tgcgccaacg ttataggtaa agcccaggcc cgctgcacgc tggtcggtat cgaaataacc
    42481 gccaatcagt ttcggtaaga tcccggcgat cccttgtcca agcatttgct ggaagaacag
    42541 taacagaccg agcacccaga cgtttgcacc gccaatagca aataccggaa taatcagcag
    42601 ctgcgaggcc aacagactac aaacgtacgc tttgcgggtt cccagccagt caccgaggaa
    42661 gccaccgacg cagcacccca ccgccgcgcc aaagccacta aagaacagca cattggcgac
    42721 agtatgaggg ttataagcca gatcggtttt cagatacgtt ggcagcagcg cctgaatcgg
    42781 ccatgagtag aggaaagcaa acaacacgac caccatcagc attacgccag ttggccagcg
    42841 tttgcccgta ctctgcacca taaagctgat aaagattgcg gtgcataaca gcccaagaac
    42901 agcgacgatc gcggcatttt gcaggttacc ggcgaagcag aaccacagcg cagtagccgc
    42961 cgccagtgtc attacgatat tggcaatgcg atgttcacca cggtagagaa tatccaccat
    43021 tgtgcgtact ggtgctttac ctgcgtgttt ctctttccag tcttctgctt ccgggatgtt
    43081 tttacgcagc cagagagcaa agatgattgg caaaatgccg ataaagaaca gcgcacgcca
    43141 gccccagacc ggaaccacca ggctgtagac ctgagcggca acgacggccc ctacagagaa
    43201 gcctgaaatc agaaagccac tggctttgtt acgcagatgt tttggccagc tttcaatgac
    43261 ataggtggcg ctggaaccgt attcacccgc catccccatg ccgatgacca gacgagcgat
    43321 aaacatggtg atgtagcctg gcgcaaagcc gcatgccagc gtcccggccg agaagagaac
    43381 gatgctggtg accattgcca gacgacgccc gtagcggtca cccatagcgc cgagcatcag
    43441 gccgccgaac cagcgagaga taaaggctgc ggagatcaga cttgccgcct gcaccgtcgt
    43501 cagcccgaat tcaccttgta cttcggtgag taccagggcg attaaaacga aatcaaaacc
    43561 gtcaagcaga tatcccaacc aggcagcgga aaatgcccgc cattgtgcac gattgagatg
    43621 gcgataccac gggatattct gggttgtagt actcattgtg agtctcccgc ggtgggcgat
    43681 gcccacacgc tttggtatga aaattgtagg gtacagatgc gtttatttcc cctcacccgg
    43741 taggggcgag cgaggggaaa caactcaccc gcgctcttgc atcaactgct gggccagcgc
    43801 cttcagttct ggcagatatt tttcatctac cggaccaaac ggtttgcggc acagcggcac
    43861 agaaacgaca tccatataat gcagaacagt tttcaggccg cggaatacac ccgttttgat
    43921 cagtaaatca atgactttgt tgcattcagt ttgcagtttc tgcgcggtct ggatatcgcc
    43981 ttctttcagc gccttaacga tcccctgata gcgccagccc atgatgttgt aggtactacc
    44041 gataccacca tcagcgcccg ccagcaggcc agaggcgaag atttcgtcgt aaccgttata
    44101 gagcaccaga tcaggatgtt cacgacggat ctgctccatc tgatagagat cgccagaggt
    44161 ctgtttcagc gcacctacgc caggcaatgt aacaagtgtg ttgatctgat ccagggtcag
    44221 ttttacccca ctcagggctg gaatgttgta caccaccatc ggcaaaccat ccgccgaatc
    44281 aataattgcc cgatagtgat cgcagtgttc ttcaaagctg aaaggatagt agaacggcgt
    44341 gacggcggag acggcatcga agccataacg tttagccgat gccgcaagtt gttggctttc
    44401 ggcggtgctg acgcaaccga cgtgggcgat gagtttaatc ttacctttcg cctcttcggc
    44461 gacgatttcc agtacctgtt cacgctcgga aaggctttgt acaaaggctt cgccggtcga
    44521 accacccacg tataaaccgt caatgccctg ctgaatattg aactgaacca ggcggcgcag
    44581 actcgcttta tccagtgctt gttgttgatc aaaaggagtc aggagtgcag ccattacgcc
    44641 acgtaaattc gttgccataa atacctctga agtgatgctt gtctgataaa cgatatacct
    44701 ttatacctgt tataccagat caattaagca acaccccata cagaaagctt ataatgcgat
    44761 ctgcttcact aaagtggcat tatttctttt tgttggtggt ctgaccgaag gcgtgccagg
    44821 tagcagagac gctgttgaga tgcgattgca acgcacgatc ggcttcgtca ggatcgtggc
    44881 ggcggatcgc gtcaacgatc gcaatatgct gttgatagct aacgttgtta tgctcgtgca
    44941 gagcctgatc agccacggtt ggacgtgcgg cgatcagcca gtcgagcagg gcaacgtgga
    45001 tcgccatgaa gattgggtta ccggggatct ccgccagcac gcggtggaaa tcaacgtctg
    45061 agcgaatgaa tgccgcgttg ttatccagcg actgactgtt gatttccagt gcttttgcca
    45121 gcaaatcgat ttgctcatcg gtggcgtgtt cagccgcata gcgcaccagg ctggattcaa
    45181 agaacagacg taattgttcg aaatgggcaa tcccaccagg atgagaaagg aaatctttcg
    45241 ccatgccgga aagctcgccg atgatggtgt ccgcagaagg acgtgagacg cgggcacgtt
    45301 cgccgttgtt tatttgcacc agacctttgc gttttaacgc tgccagcgct tcacgcaccg
    45361 aaggacgccc gacgttaaag aacgccatca gttcgcgttc agacggtaat tgttcacctt
    45421 cgccaaattc acgacggcgg atcatctgtt ccagctcttc ttccaccatt tcggagagtt
    45481 ttttacgcgc cagcgggcgg ctacgcaagt tgcgaccaat tgcaggtgaa gaatcttcgg
    45541 tttgcgaatc aaatgcgttc ataaggccca ttctgtaagg tcagtgtgat taacatcatc
    45601 agtgacatcc tatcacagga ttgaaagtag gggaaaatgg cagggttttc tctttgtgcc
    45661 tcatcattac cataattaac
//
LOCUS       DBFIFM010000012        43455 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.012, whole genome shotgun sequence.
ACCESSION   DBFIFM010000012 DBFIFM010000000
VERSION     DBFIFM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.012"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            167..1384
                     /gene="alaA"
                     /locus_tag="N7C74_000626"
     CDS             167..1384
                     /gene="alaA"
                     /locus_tag="N7C74_000626"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HGU2430565.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPCGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1468..2067
                     /gene="yfbR"
                     /locus_tag="N7C74_000627"
     CDS             1468..2067
                     /gene="yfbR"
                     /locus_tag="N7C74_000627"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HGU2430566.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2126..3958)
                     /locus_tag="N7C74_000628"
     CDS             complement(2126..3958)
                     /locus_tag="N7C74_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HGU2430567.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4045..4695)
                     /gene="hxpA"
                     /locus_tag="N7C74_000629"
     CDS             complement(4045..4695)
                     /gene="hxpA"
                     /locus_tag="N7C74_000629"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HGU2430568.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4706..5200)
                     /locus_tag="N7C74_000630"
     CDS             complement(4706..5200)
                     /locus_tag="N7C74_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HGU2430569.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5283..5738)
                     /gene="yfbV"
                     /locus_tag="N7C74_000631"
     CDS             complement(5283..5738)
                     /gene="yfbV"
                     /locus_tag="N7C74_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HGU2430570.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6076..7278
                     /gene="ackA"
                     /locus_tag="N7C74_000632"
     CDS             6076..7278
                     /gene="ackA"
                     /locus_tag="N7C74_000632"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HGU2430571.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7353..9497
                     /gene="pta"
                     /locus_tag="N7C74_000633"
     CDS             7353..9497
                     /gene="pta"
                     /locus_tag="N7C74_000633"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGU2430572.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9687..11207
                     /gene="yfcC"
                     /locus_tag="N7C74_000634"
     CDS             9687..11207
                     /gene="yfcC"
                     /locus_tag="N7C74_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HGU2430573.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRLLTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11240..11782)
                     /gene="yfcD"
                     /locus_tag="N7C74_000635"
     CDS             complement(11240..11782)
                     /gene="yfcD"
                     /locus_tag="N7C74_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HGU2430574.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11840..12391)
                     /gene="yfcE"
                     /locus_tag="N7C74_000636"
     CDS             complement(11840..12391)
                     /gene="yfcE"
                     /locus_tag="N7C74_000636"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HGU2430575.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12447..13091)
                     /gene="yfcF"
                     /locus_tag="N7C74_000637"
     CDS             complement(12447..13091)
                     /gene="yfcF"
                     /locus_tag="N7C74_000637"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HGU2430576.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLLFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPECLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13227..13874
                     /gene="yfcG"
                     /locus_tag="N7C74_000638"
     CDS             13227..13874
                     /gene="yfcG"
                     /locus_tag="N7C74_000638"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HGU2430577.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     LISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13931..14293
                     /gene="folX"
                     /locus_tag="N7C74_000639"
     CDS             13931..14293
                     /gene="folX"
                     /locus_tag="N7C74_000639"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HGU2430578.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14314..15207
                     /locus_tag="N7C74_000640"
     CDS             14314..15207
                     /locus_tag="N7C74_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HGU2430579.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGIDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNKFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15255..16145)
                     /gene="rpnB"
                     /locus_tag="N7C74_000641"
     CDS             complement(15255..16145)
                     /gene="rpnB"
                     /locus_tag="N7C74_000641"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HGU2430580.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYLYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDVGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLEKGFD
                     HEVILTLTRLSPDDLIAQSH"
     gene            complement(16342..17115)
                     /gene="hisP"
                     /locus_tag="N7C74_000642"
     CDS             complement(16342..17115)
                     /gene="hisP"
                     /locus_tag="N7C74_000642"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HGU2430581.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17123..17839)
                     /locus_tag="N7C74_000643"
     CDS             complement(17123..17839)
                     /locus_tag="N7C74_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2430582.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17836..18522)
                     /gene="hisQ"
                     /locus_tag="N7C74_000644"
     CDS             complement(17836..18522)
                     /gene="hisQ"
                     /locus_tag="N7C74_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HGU2430583.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAARAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18612..19394)
                     /gene="hisJ"
                     /locus_tag="N7C74_000645"
     CDS             complement(18612..19394)
                     /gene="hisJ"
                     /locus_tag="N7C74_000645"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HGU2430584.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19615..20397)
                     /gene="argT"
                     /locus_tag="N7C74_000646"
     CDS             complement(19615..20397)
                     /gene="argT"
                     /locus_tag="N7C74_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HGU2430585.1"
                     /translation="MKKSILALSLLVGISAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20663..21232)
                     /gene="ubiX"
                     /locus_tag="N7C74_000647"
     CDS             complement(20663..21232)
                     /gene="ubiX"
                     /locus_tag="N7C74_000647"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HGU2430586.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21327..22844)
                     /gene="purF"
                     /locus_tag="N7C74_000648"
     CDS             complement(21327..22844)
                     /gene="purF"
                     /locus_tag="N7C74_000648"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HGU2430587.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22881..23369)
                     /gene="cvpA"
                     /locus_tag="N7C74_000649"
     CDS             complement(22881..23369)
                     /gene="cvpA"
                     /locus_tag="N7C74_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HGU2430588.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGLLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23901..24563)
                     /gene="dedD"
                     /locus_tag="N7C74_000650"
     CDS             complement(23901..24563)
                     /gene="dedD"
                     /locus_tag="N7C74_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HGU2430589.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVVPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24553..25821)
                     /gene="folC"
                     /locus_tag="N7C74_000651"
     CDS             complement(24553..25821)
                     /gene="folC"
                     /locus_tag="N7C74_000651"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HGU2430590.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVCYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKTLPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25891..26805)
                     /gene="accD"
                     /locus_tag="N7C74_000652"
     CDS             complement(25891..26805)
                     /gene="accD"
                     /locus_tag="N7C74_000652"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HGU2430591.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26961..27620)
                     /locus_tag="N7C74_000653"
     CDS             complement(26961..27620)
                     /locus_tag="N7C74_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGU2430592.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27703..28515)
                     /gene="truA"
                     /locus_tag="N7C74_000654"
     CDS             complement(27703..28515)
                     /gene="truA"
                     /locus_tag="N7C74_000654"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HGU2430593.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28515..29528)
                     /locus_tag="N7C74_000655"
     CDS             complement(28515..29528)
                     /locus_tag="N7C74_000655"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGU2430594.1"
                     /translation="MSEGWNIAVLGTTGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFVQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29594..30730)
                     /gene="pdxB"
                     /locus_tag="N7C74_000656"
     CDS             complement(29594..30730)
                     /gene="pdxB"
                     /locus_tag="N7C74_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HGU2430595.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADCGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30829..31824
                     /gene="flk"
                     /locus_tag="N7C74_000657"
     CDS             30829..31824
                     /gene="flk"
                     /locus_tag="N7C74_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HGU2430596.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHCPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQSEAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31821..32999)
                     /locus_tag="N7C74_000658"
     CDS             complement(31821..32999)
                     /locus_tag="N7C74_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2430597.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33274..34494)
                     /gene="fabB"
                     /locus_tag="N7C74_000659"
     CDS             complement(33274..34494)
                     /gene="fabB"
                     /locus_tag="N7C74_000659"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HGU2430598.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34653..36659
                     /gene="mnmC"
                     /locus_tag="N7C74_000660"
     CDS             34653..36659
                     /gene="mnmC"
                     /locus_tag="N7C74_000660"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HGU2430599.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     ISQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNGFTFAR
                     RLYDSLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPEELAVAVEANAVEQITGVT
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQDLSRKDDCWLLTFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETAYSEDNQQQNRQRLIDCFPHAQWAKTVDVSKKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDKAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36780..37058)
                     /locus_tag="N7C74_000661"
     CDS             complement(36780..37058)
                     /locus_tag="N7C74_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HGU2430600.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(37092..37640)
                     /gene="epmC"
                     /locus_tag="N7C74_000662"
     CDS             complement(37092..37640)
                     /gene="epmC"
                     /locus_tag="N7C74_000662"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HGU2430601.1"
                     /translation="MNSTHHYEQLIEIFNSCFADDFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARREQVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37640..38449)
                     /locus_tag="N7C74_000663"
     CDS             complement(37640..38449)
                     /locus_tag="N7C74_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HGU2430602.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38449..39273)
                     /gene="mepA"
                     /locus_tag="N7C74_000664"
     CDS             complement(38449..39273)
                     /gene="mepA"
                     /locus_tag="N7C74_000664"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HGU2430603.1"
                     /translation="MNKTAIALLALLASSVSLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRRTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39277..40362)
                     /gene="aroC"
                     /locus_tag="N7C74_000665"
     CDS             complement(39277..40362)
                     /gene="aroC"
                     /locus_tag="N7C74_000665"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HGU2430604.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40397..41329)
                     /gene="prmB"
                     /locus_tag="N7C74_000666"
     CDS             complement(40397..41329)
                     /gene="prmB"
                     /locus_tag="N7C74_000666"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGU2430605.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKAQLLAAREHFAIYKD"
     gene            41495..42046
                     /gene="smrB"
                     /locus_tag="N7C74_000667"
     CDS             41495..42046
                     /gene="smrB"
                     /locus_tag="N7C74_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311241.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HGU2430606.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKVSEVPV
                     KRLIQEQVDASHYFSDEFQPLLNADGPVKYVRPGVDHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42217..43059)
                     /locus_tag="N7C74_000668"
     CDS             complement(42217..43059)
                     /locus_tag="N7C74_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HGU2430607.1"
                     /translation="MKKWTIFLTSLILLALSLETPKCYAGDKLMSASFSSTKIYYAMK
                     NVTASGSLYFYVTVVTPGEVSYGQYNSNARKGDTLKLISWSGSGPAPTLVLTDYRRTD
                     TSNCPGINTRVFVCAYMTFNVTVESDNYGCPWIASFYSVSEAFGFGTYTSPTVHDSIC
                     PTIPVASFDISWSDNYVSHNKALRLQSDGSTITTTLSTYLMESGKLCDGSIFDSRGAY
                     CRAVSDLLTFTSYGCDNAKVTVTPSRQPLTDRKLHDIVVQVNTSSREPIDSTCRFQYV
                     LNEL"
     gene            complement(43061..>43455)
                     /locus_tag="N7C74_000669"
     CDS             complement(43061..>43455)
                     /locus_tag="N7C74_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2430608.1"
                     /translation="NLLAEIHFPTIAASDLMQRGQSDRVPLVFQLKDCKSTTAFNVKV
                     TLMGTEDTDLPGFLSIDSSSSATGVGIGIETAGGAAVPINSTTGASFPLNQGNNSVNF
                     NAWLQTVNGRNVTSGDFTATMTVTFEYF"
BASE COUNT        10742 a        11737 c        10850 g        10126 t
ORIGIN      
        1 acctcattat tttgtcatta ttaagttatt aacagcacaa tcgagccttc ccctctggca
       61 aaatcttatt ctgcagacct tcaaaacacc gtcctggggg agtacattgt tctaagctga
      121 cttccacggc agggagtggc gataacagca aaaaaggtca agattcatgt cccccattga
      181 aaaatccagc aaattagaga atgtctgtta tgacatccgt ggtccggtgc tgaaagaagc
      241 aaaacgcctg gaagaagaag gtaacaaggt actgaaactg aacatcggca acccagcccc
      301 gttcggtttt gacgcgccag atgaaatcct cgttgacgtg atacgcaacc tgcctaccgc
      361 tcaagggtat tgcgattcca aaggtcttta ctccgcgcgt aaagccatca tgcagcacta
      421 tcaggctcgt ggcatgcgtg atgttaccgt ggaagatatt tacatcggca atggtgtatc
      481 ggagcttatc gttcaggcaa tgcaggcatt gctgaacagc ggggacgaaa tgttggttcc
      541 tgcaccagat tacccactat ggaccgcggc ggtttcgctt tccagcggta aagcggtgca
      601 ttatctttgc gatgaatcct ctgactggtt cccggacctc gatgatattc gcgctaaaat
      661 tacgcctcgt acgcgtggga tcgttattat caacccaaat aacccaaccg gcgcggtata
      721 ttccaaagag cttttaatgg agattgtgga gattgcacgt cagcataatc tcattatctt
      781 cgccgatgaa atttatgaca aaattctcta cgacgacgct gagcatcact caattgcgcc
      841 gctggcacct gacctgctga ccattacctt taatggactg tcgaaaacgt accgcgttgc
      901 aggcttccgt caggggtgga tggtgttgaa cgggccgaaa aaacacgcca aaggctacat
      961 cgaaggtctg gaaatgctgg cttcaatgcg cctgtgtgct aacgttcctg cgcaacacgc
     1021 cattcagacc gcgctgggtg gttatcagag catcagtgaa tttattaccc ctggcggtcg
     1081 tctttatgag cagcgtaacc gcgcgtggga attgatcaac gatattccgg gcgtttcctg
     1141 cgtgaaacct tgtggtgcgc tgtacatgtt cccgaaaatc gacgccaaac gctttaacat
     1201 tcacgacgat cagaaaatgg tgctggattt cctgttgcag gaaaaagttc tgttggtgca
     1261 agggacggca ttcaactggc cgtggccgga tcacttccgc attgtcacgc taccgcgtgt
     1321 cgatgatatc gagctgtcgt tgagcaagtt cgcgcgtttc ctttctggtt atcatcagct
     1381 gtaatcttaa tttcactgcc ggagattgca tccggcagtg ttatcccgcc acaatgacct
     1441 gatgatgtca tcatacgtaa ggtcactatg aaacagagcc atttttttgc ccatctctcc
     1501 cgcctgaaac tcattaaccg ctggccgctc atgcgcaacg tgcggacgga aaatgtgtcc
     1561 gaacacagtt tgcaggtagc gatggtcgcc catgcgctgg cagctatcaa aaatcggaaa
     1621 tttggcggta atgtcaacgc cgaacgtatc gctttactgg cgatgtacca cgatgcctca
     1681 gaagtgctca ccggcgatct ccctactccg gtgaaatact tcaattcgca aatcgctcag
     1741 gagtacaagg ctattgaaaa aatcgctcag caaaaactgg tcgatatggt tccggaagag
     1801 ctgcgggata tctttgcgcc gttaattgac gagcatgcat atagcgatga agaaaaatcg
     1861 ctggtgaaac aggcagatgc actgtgtgca tatctgaaat gtctggaaga actcgcggcg
     1921 ggaaataatg aattcttgct ggcaaaaacg cgactggaag cgacgcttga agcgcgccgc
     1981 agccaggaga tggactactt catggaagta tttgttccca gcttccatct ttcgctcgat
     2041 gagattagcc aggattcacc gctgtaagca gctggagtct gcgtcgcatc aggcaataag
     2101 cgccggatgc gacatcaggc tcttgtcaaa acggaaacag catcgggatc atcaccacac
     2161 aaaccgccat cacgataatg gtgaacggta cccccagctt cacaaagtca ctgaagctgt
     2221 aatttcccgg acctaaaacc aatgtgttaa caggtgaaga aaccggtgtc ataaaggcgg
     2281 cggatgctgc catcgccacg accatcgcga atggataagg cgacaccccc atcgttttgg
     2341 cagcagccag cgcaatcgga gccatcaaca ccgccgtcgc ggtattagag ataaatagcc
     2401 caataaccgc cgacaagaca aacaaacaac ccagcatcat atgtggcccg taaccaccgc
     2461 caatgtccat cagccctttc accgccagcg cgacacctcc cgttttctgt aatgccacag
     2521 caaacggcat catcccaacg atcaaaataa tgctcggcca gtgaatggat ttataggcgc
     2581 tttcagcatc tatacagcgg aatttcccca tcagcaggca ggcgatgata gcggcaacag
     2641 gattaggaat ttcatctgtc agcattaacg ccaccattag caccagacag aaaatggcat
     2701 ggggtgcctg gctgtgcgcg ggcgatgctt cactcacctc ttccggtaag ttcagcgcta
     2761 cgaagtcgcg gccctgtttg gccagcatac cgatcagttt ccagttaccc acaaccagga
     2821 tgatatcgcc cagcagcaga ggctcatccg ccagcgaacc ttccagcgcc acgccattgc
     2881 gctttagccc caccacattc agtccgtagc gggtacgaaa accaatttcg cgcaccgatt
     2941 taccaatcag ttctgactca ggaattaatg aaatctctgc catgcccaca tcaagggcct
     3001 ggtcagaaaa atactcgccg cgcagtacca tcggctccag caattgctca ctacaaaatt
     3061 gccggagatc gacatcagcc gcagacatat caataagcaa aacgtcacgc gcgcgaaatt
     3121 cagaaacccc attaacgttc acgataacgc gacgaaaacg ccgccagcgt tcaacaccga
     3181 tgacgttagc gccataacgc tcacgtaatt tgagatcatc cagccgttga ccaatcattg
     3241 gcgatccggg gcgaatagcc agacgtcgcg cacgcccggt cagtcgatat tcacggataa
     3301 gatcgcgaaa ggttcgacgc gtccagcctt cgcgctgcgg ggtctgggta tcccctttca
     3361 gcatgaaacg catcactaac atatacaaaa tacccagcac cagcacgacc aggccaatag
     3421 gtgttacgct aaagaaactg aagccgtgat agccttcacg cagcaattca ctgttgacta
     3481 ccaggttcgg cggcgtcgcc accagcgtca tcatgccgct aatcagcccg gcaaaactta
     3541 acggcatcat cagacgtgac ggcgaagttt gcatacgcat ggcaacgctt aacaccacgg
     3601 gaataaagat agcgacaacg ccggttgaac tcataaacgc gccaagcccc gcgacggtca
     3661 gcatcaacaa aaccaacatt ttgatttcac tgttgcccgc aactttgacc agccatgttc
     3721 ccattacggt ggcaacaccg gtacggacca aaccatcgcc aataataaac aaggcggcaa
     3781 tcaggacaac gttaggatca gaaaagccag aaaatacttc tgggactgtc agcgttccgc
     3841 ttaatgcaaa cgcgacaata acaaacaaag cgaccgcatc catacgcact ctgcccgtcg
     3901 caaacaagac gatggcaacg gccagtaatg aaagaaccca aatcaattca ccgttcacaa
     3961 cttatccttg ttaattgagg gggatgactt gattctgcca taaaaaagcc ccgacgagac
     4021 ggggctaaat catgatcagg tgtttcactg aataataaca tcgccatttg gctgtttggt
     4081 cacagtaatt tgctccagac tgtggaggac caaatcgacc tcattcaggc gcggggtatc
     4141 tgccggagcg ttaaccgcaa tgacatggca acccgccgcc aggccagaaa gcacgccagc
     4201 gggagcatct tccaccacca cacactcctg cggcgcaagc cccagcagct gcgcgcctaa
     4261 cagatacgca tcaggttctg gttttccgcg cttcactcgc tcagcggtta caaacacctc
     4321 tggtgcggga agccccgcta ttttatggcg cgctcgcgct accggcatgg agccagaagt
     4381 cacaatggcc cacggaatac ctgctttatt caaatgattg agtaaggcga ttgcccccgg
     4441 aagcgcggta ataccttcgg tttccgtggc ctcgatttgc tccaaacgcg taaactcggc
     4501 ggcaatatca gcctcggatt tgcccgccat aaaatggcgc agagaggtga tcgcctgttt
     4561 accgtgaatg aaagccagca cctcttccgg cgctaaccca tgacgtctgg cccagttgct
     4621 ccacgcccgt tctaccgcag gcagggaatc caccagcgtt ccatcaagat caaacagaaa
     4681 acctttgcac cgcacgcggg cctcctcagg cgttgataat ttgattaatt tcgttggcgc
     4741 tcaaatggta ctgacgcggg caggcatgcc acacattaag catgcgctga tatttttccc
     4801 acattggcgt ctgggcgtta aaaccgtgag ttccggcgtc aaaatgggta tagcgccctt
     4861 ccacattaac cataaagcgg acataaccga ggtaacgtgc ttcagtggcg gcgtcaaagc
     4921 cgaggaaggt gacacgacgt tcatcgatgg attgctggtc ctgcaaatta gaccaggaaa
     4981 catgcaacgc atgatacatc tccataatgt cgatgatagt gcggcaggtt tcttctttca
     5041 gctcgccaaa ctcgcgatcc agttcacgca tctgtaatcc ataaccccgc tcaatgattg
     5101 tttgcaggcg acggtaacgt tcagcatttg ccggatcgag catagtcatc atcttgtact
     5161 ggttagacaa aataagacgt tgcgcgttgg tcatttccat tgttgactcc tgtatcactc
     5221 tactacggtg aaaaaaaaga aggctgagta tgccttcttt tatatgcgta atcaggggtc
     5281 aattacaaat catcaaggaa agttttatcc agttgtttga aggcacgctt aagcgtgtca
     5341 gctaatgcct ggtaatcagg cttgccttca acgggtgcca acacctgtcc agactcctgc
     5401 aatttaccgc gaacttcata aaaccagttg aggattgcag ggggtaatgg cgtgacagaa
     5461 cgcttgccca gccaccacaa tccctgcatg ggtaaactta aggcgaacag ggcagtggca
     5521 actgccggcc caagctgacc gcccagggca atctgccagc agagagtaaa tacggcgatc
     5581 ggcggcataa aacggatcgc ataacgcgtc atcttgataa cgcgattttc gacaaagacc
     5641 ggggcaaggc gtttttccag cggccacgtc tttgagtaat gctgtccccg gcgaaacaag
     5701 ctaaaaaaat taacagaacg attatccggc gttgacatgc ttcacctcaa cttcacatat
     5761 aaagattcaa aaatttgtgc aaattcacaa ctcagcggga caacgttcaa aacattttgt
     5821 cttccatacc cactatcagg tatcctttag cagcctgaag gcctaagtag tacatattca
     5881 ttgagtcgtc aaattcatat acattatgcc attggctgaa aattacgcaa aatggcatag
     5941 actcaagata tttcttccat catgcaaaaa aaatttgcag tgcatgatgt taatcataaa
     6001 tgtcggtgtc atcatgcgct acgctctatg gctccctgac gtttttttag ccacgtatca
     6061 attataggta cttccatgtc gagtaagtta gtactggttc tgaactgcgg tagttcttca
     6121 ctgaaatttg ccatcatcga tgcagtaaat ggtgaagagt acctttctgg tttagccgaa
     6181 tgtttccacc tgcccgaagc acgtatcaaa tggaaaatgg acggcaataa acaggaagcg
     6241 gctttaggtg caggcgccgc tcacagcgaa gcgctcaact ttatcgttaa tactattctg
     6301 gcacaaaaac cagaactgtc tgcgcagctg actgctatcg gtcaccgtat cgtacacggc
     6361 ggcgaaaagt ataccagctc cgtagtgatc gatgagtctg ttattcaggg tatcaaagat
     6421 gcagcttctt ttgcaccgct gcacaacccg gctcacctga tcggtatcga agaagctctg
     6481 aaatctttcc cacagctgaa agacaaaaac gttgctgtat ttgacaccgc gttccaccag
     6541 actatgccgg aagagtctta cctctacgcc ctgccgtaca acctgtacaa agagcacggc
     6601 atccgtcgtt acggcgcgca cggcaccagc cacttctatg taacccagga agcggcaaaa
     6661 atgctgaaca aaccggtaga agaactgaac atcatcacct gccacctggg caacggtggt
     6721 tccgtttctg ctatccgcaa cggtaaatgc gttgacacct ctatgggcct gaccccgctg
     6781 gaaggtctgg tcatgggtac ccgttctggt gatatcgatc cggcgatcat cttccacctg
     6841 cacgacaccc tgggcatgag cgttgacgca atcaacaaac tgctgaccaa agagtctggc
     6901 ctgctgggtc tgaccgaagt gaccagcgac tgccgctatg ttgaagacaa ctacgcgacg
     6961 aaagaagacg cgaagcgcgc aatggacgtt tactgccacc gcctggcgaa atacatcggt
     7021 gcctacactg cgctgatgga tggtcgtctg gacgctgttg tattcaccgg tggtatcggt
     7081 gaaaatgccg cgatggtccg tgaactgtct ctgggcaaac tgggcgtgct gggctttgaa
     7141 gttgatcatg aacgcaatct ggctgcacgt ttcggcaaat ctggtttcat caacaaagaa
     7201 ggtacccgtc ctgcggtggt tatcccaacc aacgaagaac tggttatcgc gcaagacgcg
     7261 agccgcctga ctgcctgatt tcacaccgcc agctcagctg gcggtgctgt tttgtaaccc
     7321 gccaaatcgg cggtaacgaa agaggataaa ccgtgtcccg tattattatg ctgatcccta
     7381 ccggaaccag cgtcggtctg accagcgtca gccttggcgt gatccgtgca atggaacgca
     7441 aaggcgttcg tctgagcgtt ttcaaaccta tcgctcagcc gcgtaccggt ggcgatgcgc
     7501 ccgatcagac tacgactatc gtgcgtgcga actcttccac cacgacggcc gctgaaccgc
     7561 tgaaaatgag ctacgttgaa ggtctgcttt ccagcaatca gaaagatgtg ctgatggaag
     7621 agatcgtcgc aaactaccac gctaacacca aagacgctga agtcgttctg gttgaaggtc
     7681 tggtcccgac acgtaagcac caatttgccc agtctctgaa ctacgaaatc gctaaaacgc
     7741 tgaatgcgga aatcgtcttc gttatgtctc agggcactga caccccggaa cagctgaaag
     7801 agcgtatcga actgacccgc aacagcttcg gcggtgccaa aaacaccaac atcaccggcg
     7861 ttatcgttaa caaactgaac gcaccggttg atgaacaggg tcgtactcgc ccggatctgt
     7921 ccgagatttt cgacgactct tccaaagcta aagtaaacaa tgttgatccg gcgaagctgc
     7981 aagaatccag cccgctgccg gttctcggcg ctgtgccgtg gagctttgac ctgatcgcga
     8041 ctcgtgcgat cgatatggct cgccacctga atgcgaccat catcaacgaa ggcgacatca
     8101 atactcgccg cgttaaatcc gtcactttct gcgcacgcag cattccgcac atgctggagc
     8161 acttccgtgc cggttctctg ctggtgactt ccgcagaccg tcctgacgtg ctggttgccg
     8221 cttgcctggc tgccatgaac ggcgtagaaa tcggtgccct gctgctgact ggcggctacg
     8281 aaatggacgc gcgcatttct aaactgtgcg aacgtgcttt cgctaccggc ctgccggtat
     8341 ttatggtgaa caccaacacc tggcagactt ctctgagcct gcagagcttc aacctggaag
     8401 ttccagttga cgatcacgag cgtatcgaga aagttcagga atacgttgct aactacatca
     8461 acgctgactg gatcgattct ctgactgcca cttctgagcg cagccgtcgt ctgtctccgc
     8521 cagcgttccg ttatcagctg actgaacttg cgcgcaaagc gggcaaacgt atcgtactgc
     8581 cggaaggtga cgaaccgcgt accgttaaag cagccgctat ctgtgctgaa cgtggtatcg
     8641 caacttgcgt actgctgggt aatccggcag agatcaaccg tgttgcagcg tctcagggtg
     8701 tagaactggg tgcaggcatt gaaatcgttg atccagaagt ggttcgcgaa agctatgttg
     8761 gtcgtctggt cgagctgcgt aagaacaaag gcatgaccga aaccgttgcc cgcgaacagc
     8821 tggaagacaa cgtggtgctc ggtacgctga tgctggaaca ggatgaagtt gatggtctgg
     8881 tttccggtgc tgttcacact accgcaaaca ccatccgtcc gccgctgcag ctgatcaaaa
     8941 ctgcaccggg cagctccctg gtatcttccg tgttcttcat gctgctgccg gaacaggttt
     9001 acgtttacgg tgactgtgcg atcaacccgg atccgaccgc agaacagctg gcagaaatcg
     9061 cgattcagtc cgctgattcc gctgcagcct tcggtatcga accgcgcgtt gctatgctct
     9121 cctactctac cggtacttct ggtgcaggta gcgacgtaga aaaagttcgc gaagcaactc
     9181 gtctggcgca ggaaaaacgt cctgacctga tgatcgacgg tccgctgcag tacgacgctg
     9241 cggtaatggc tgacgttgcg aaatccaaag cgccgaactc tccggttgca ggtcgcgcta
     9301 ccgtgttcat cttcccggat ctgaacaccg gtaacaccac ctacaaagcg gtacagcgtt
     9361 ctgccgacct gatctccatc gggccgatgc tgcagggtat gcgcaagccg gttaacgacc
     9421 tgtcccgtgg cgcactggtt gacgatatcg tctacaccat cgcgctgact gcgattcagt
     9481 ctgcacagca gcagtaacct cgtcatcatc cgcagctttg cgctgcggat atctgaaccg
     9541 gaaatagtca ctatttccgg ttttttattc tcttaatttg cattaatcct ttctgattat
     9601 cttgcttaac tgcgctgcat caatgaattg cgccatctca ctttgcatac ttaccacttt
     9661 gttttgtgca agggaatatt tgcgctatgt ccgcaatcac tgaatccaaa ccaacaagaa
     9721 gatgggcaat gcccgatacg ttggtgatta tcttttttgt tgctatttta accagccttg
     9781 ccacctgggt agttccggtg gggatgtttg acagtcagga agtgcagtat caggttgatg
     9841 gtcaaacaaa aacacgcaaa gtcgtagatc cacactcatt tcgccttctg actaacgaag
     9901 caggcgaacc tgagtatcac cgcgtacagc tgttcacgac gggcgatgaa cgcccgggcc
     9961 tgatgaactt cccgtttgaa gggttaacct caggatcgaa atacgggaca gccgttggca
    10021 tcatcatgtt tatgctggtg attggcggcg cgtttggcat tgtgatgcgt acaggaacca
    10081 ttgataacgg tatcctggcg cttattcgcc atacccgtgg gaatgaaatt ctctttattc
    10141 ctgcgctgtt tattctgttt tcacttggcg gcgcggtatt tggtatgggg gaagaggccg
    10201 tcgcctttgc cattatcatc gcaccgctaa tggtccggct gggctatgac agtattacca
    10261 ccgtcctggt gacctatatt gccacgcaaa tcggttttgc cagttcgtgg atgaacccgt
    10321 tttgtgtggt cgttgctcag gggattgccg gcgttccggt gctttctggc tccgggttgc
    10381 gcatcgtggt gtgggttatc gccactctga ttggcctgat ctttaccatg gtgtacgcct
    10441 cacgagtgaa aaagaatcct cttctgtcac gcgtgcatga gtccgaccgc ttctttcgtg
    10501 aaaagcaggc ggatgttgaa caacgtccgt ttacctttgg tgactggctg gtattgattg
    10561 tcctgaccgc cgtaatggtc tgggtgattt ggggcgtgat cgttaatgcc tggtttattc
    10621 cagaaattgc cagccagttc ttcaccatgg gtctggtgat tggcatcatc ggcgttgttt
    10681 tccgccttaa cggtatgacg gttaatacca tggcttcatc cttcaccgaa ggggcgcgaa
    10741 tgatgatcgc ccctgccctg ctggtgggtt tcgccaaagg gattttgctg ctggtcggta
    10801 atggtgaagc gggtgatgcc agcgtgttaa atactatcct caacagcatt gccaatgcca
    10861 ttagcggtct ggacaacgcg gtcgcggcct ggtttatgtt gctcttccag gcggtattta
    10921 atttcttcgt gacgtccggt tctggtcagg cggcgttaac catgccgtta ctggcaccgc
    10981 ttggcgatct ggtcggtgtt aaccgtcagg ttaccgtgct ggctttccag tttggtgatg
    11041 gcttcagtca catcatttac ccgacctcag cttcgttaat ggcgacgctc ggtgtttgcc
    11101 gggtggactt ccgtaactgg ctgaaggtgg gcgcgaccct gcttggactg ctgtttatta
    11161 tgtccagcgt cgtggtgatc ggcgctcagt tgatgggcta ccactaaaaa tgttaagagc
    11221 cgcaattgcg gctctttttt cattctgccg tttcagtctc tacggcttca tttttggcat
    11281 tgcgcttcat ccacaacgct agcgctttca gcgagtctgg agtgaactca tcgcagcgtg
    11341 cggtgatttc ttccggcgtc agccagcaaa cttcactgac ttcatcttcc tgtagggcga
    11401 agggaccgtg agagacgcag ctgaacaatg cgccccagac acggcaattt ttatcttcga
    11461 aatagaactg cccgtgctcg gcaaagggga caccggcaat gcccaactct tcttccgctt
    11521 cgcgacgcgc ggattccagc agttgctcat cggcctggac tacaccgcca gcggtcgcat
    11581 ctaacatgcc gggtaaaaag tcttttgtct cggtacgacg ctggaccaga attttgccca
    11641 tgccatcatg cacgacgatg taagttgcac gatgacgcag acactgtgcc cgcatttgct
    11701 cccggctggc ttgtgcaatg acttcgttct cttcattgac aatatccacc cattcagtac
    11761 ttgccaaacg acgctgttcc attatcagga aaccttcttt ttctggcgct cttacggcgc
    11821 attttgagtt gtgggtaaat tacggattaa tcgcgacctg cgcaatgata ctttgatcat
    11881 tgagtgcgat tacactaagt acgtcattat ccagcatgcc ataactcgcc ggattaccgc
    11941 ctttcggaat actcaccgag ccggggttga agtggaaaat ctccccccgc tgttctgcta
    12001 ctggcagatg ggtatgaccg taaaccagca catcgttctg gtttaaagca ggcagatttt
    12061 ccgggccaaa aagatgacca tgcgtcaaaa acagacgttg tttttccagt aatacctgtt
    12121 gccacggcgc ggttatcggg aaatgcagca gcatttgatc cacttcgctg tcgcagttgc
    12181 cgcgcacagc gataacctta tgtgccactt cattaagccg ttcagcgact ttggctggcg
    12241 cgtaaccctc cggtaaagca ttacgcgggc cgtgattcaa cacgtcgcca aggatcacca
    12301 gccactgggc accgctttgg gcaaacaact ccagaacacg ttccgtcgcc ggtaacgacc
    12361 catgaatgtc cgatgcaaac atcagtttca tcactcactc ctcgtcgaaa aaacgtaagc
    12421 tatgatactg gattctgtcg ttgctatcag ccagattgct tcgctgaaag tgcaataaaa
    12481 cgctggacag acgctcgctg ccactggaat gttgcataat ccaccaggca ttctggcacc
    12541 tcatcgccat gtagtaccag gcggttaatc attagcgcca gatcagtatc agcaatgcac
    12601 cattcaccaa ataaattcgg ctgacccagt actaacaaat gttctgccat cgcgaacagt
    12661 ttctctgcac tggcttttcc ctcggccgtt agtggcgctt ttttcgcccc cgcaaagaca
    12721 acatctgtcg gacgctcttc gcggatgggc atcagatcgc tgcgcaacca ggcctgaatc
    12781 tgtcgtgcac gcgcacgatt ttctaaatca agcggataaa tacgttccca ggtcggtggc
    12841 gcaaatcgat cttccagata ctccgcaatg gcagaagatt cactcaactc aaaatcatcg
    12901 atttgtaata acggcacacg gcgtgtctga ccgtaacctt gccacgtcgg ttgcaaatgt
    12961 tcaccgctgt cgaggtcgat ggtcttgata tgaaacaaca ggcctttttc ctgcaacgcc
    13021 acccaagcgg ataacacata aggggagaaa aaatgggcat ctgaccaaag cgtgatagcg
    13081 ggtttactca ttatgtcctc ggcaataaag ggggctggct atcaaactat agagcctttt
    13141 tgtcactgtc acgtgacgaa tactcacgta agtcgccgcg ctttctataa ttgtggtgtt
    13201 cagatgatta gacaaccgga gctccaatga tcgatctcta tttcgccccg acacccaatg
    13261 gccacaaaat tacgctgttt ctcgaagaag cagggctgga ttatcgcttg ataaaggtag
    13321 acctggggaa aggcggtcag tttcgcccgg aatttttgct catttcgcct aacaacaaaa
    13381 ttccggcaat tgttgatcat tctccagccg atggcggcga accgctaagc ctctttgaat
    13441 ctggtgccat tttgttgtat ctggctgaga aaacggggct ctttttgagt catgaaacgc
    13501 gtgaacgcgc cgccacatta cagtggttat tctggcaggt aggcggactg gggccgatgc
    13561 ttgggcaaaa tcatcatttt aatcacgcag ccccccaaac cattccttac gctattgaac
    13621 gttatcaggt tgaaactcag cgtctgtacc atgtactgaa caagcggctg gaaaactcgc
    13681 cctggctggg aggcgagaac tacagcattg cggatattgc ctgctggccg tgggttaatg
    13741 cctggactcg ccagcgaata gacctcgcta tgtatccggc agtcaagaac tggcatgagc
    13801 ggatccgttc gcgccctgcc accgggcagg cactgctaaa agcacaactc ggtgatgagc
    13861 gttcggatag ttaacagaaa caggttctcg tgtattattt catcctaagt aaaacaacgg
    13921 agaacctgca atggcacaac ctgccgctat tattcgtata aagaaccttc gtttacgtac
    13981 atttatcgga attaaggaag aagaaattaa caaccgtcag gatattgtta tcaatgtgac
    14041 gatccactac cccgccgata aagcgcgcac tagcgaagat atcaacgatg cgctgaatta
    14101 tcgcaccgta acgaaaaaca ttattcagca tgtagagaat aaccgtttct ctttgctgga
    14161 aaaattaact caggatgtgc tcgatatcgc acgtgaacat cactgggtga cgtatgctga
    14221 agtggagatc gataaactgc acgcgctgcg ctacgccgat tcggtatcca tgaccttaag
    14281 ctggcagcgt taaccgccat atcgggaggc tgcatgaaca tagtgatcac cggagggacg
    14341 ggattaattg gtcgccattt gattccacgt ttgctggagc tgggccatca aatcacggta
    14401 atgacgcgta acccgcagaa agccagttcc gttctcggcc ctcgggtgac actatggcaa
    14461 gggcttgccg atcaaagcaa cctcaacggc attgatgcgg taatcaacct ggccggagaa
    14521 ccgattgctg ataaacgttg gactcacgag caaaaagagc gtctctgcca aagccgctgg
    14581 aatatcacgc aaaaactggt cgatttgatt aatgccagcg acacgccacc gtcggtactc
    14641 atttccggct cggcaacggg ctattatggc gacttaggtg aagtggtggt taccgaagag
    14701 gaaccgccgc ataacaaatt tacccataaa ctctgcgccc gctgggaaga aattgcctgt
    14761 cgggcgcaaa gtgacaaaac gcgagtgtgc ctgctgcgta ccggcgtagt gctggcaccg
    14821 gatggcggta ttctcggtaa aatgctgccg ccgtttcgtc ttggcctggg cgggccgatt
    14881 ggttccggtc ggcagtatct ggcctggatt catatcgatg atatggtcaa cggcattctc
    14941 tggctgctgg ataacgagct gcgcgggcca tttaatatgg tttcgcccta cccgatacgc
    15001 aatgaacaat ttgcccatgc gctcggtcat gcgctgcatc gcccggctat tttgcgcgtc
    15061 cccgcaaccg ccattcggct gttaatgggc gaatcttcag tactggtatt aggtggacaa
    15121 cgcgcgctgc ctaaacggct ggaagaagcg ggttttgcgt ttcgctggta cgatttagaa
    15181 gaggcgctgg cggatgtcgt tcgctgatgt ggtttactgc aaacatccgc cagttaactc
    15241 ccggtgttac agggttagtg gctttgcgcg ataagatcgt ctggtgaaag tcgggtcagc
    15301 gtcaggatga cttcgtggtc aaatcctttt tccagcatct cctgagcaat acgcagagct
    15361 tcttcgtgtt tgccctgcat tgcgccttct tcacgtaatc tgtcagcaat ggtcatcagt
    15421 ttctcctttt cttgtggtgc gcgttctgtt atctcaccaa taaatgcacg gaaacgctgg
    15481 gcatctcccg tttgtaatac ataattaaac agggctttta gctgtctgtc attagtgttc
    15541 cctgtaacta gcagcgaaac aatttggtcg actaatccca acagatcgcg ctgacgaata
    15601 tgtttctgaa ttaactccaa cagcgccatt ttacgatgtt gcataatctc gtcatccggc
    15661 accacggtaa tatccaccaa cggaaaagcc gatgaatata ttttacgggc aatcgcaggc
    15721 tcggcaaatt catcaagcca gcagagtgaa taaggataag gacttctgca accatgataa
    15781 aacagcatcg ggatcaccaa tggaagctct ttatagccca catcaagatg gttttgcatt
    15841 gccgcaatgg aataacgcat catgcgaaaa gccattaatt cttccggctt actttggtgc
    15901 tctatcacta cataaagata acccactccc tcctgcgttt tcacagacca caagaggtcg
    15961 gaataatatt gccgcaggtc ttcatcaata aaactgtttg gttccagttt aagcgtcgtt
    16021 aaatcacaca gtttgcgcag cggcgcggga agatgaatat caataaaatc ccgcgcggtg
    16081 tctggatggc gtaaaaaaga tttaaatacc gcatcatgcg gcgtggaagt tgtcgatatt
    16141 gtcatggcgt tccgtcaccc ctcaaattta agatgacgcg acaataacca cgattaacac
    16201 ccaatgcagc aggcagtttt tatctttgga cagcgaattc agaagtattt ttagcggact
    16261 gcaactttta catttcagcg gaagctgact cgcaaattcc ccttgaaaat actccggcag
    16321 cgttagccac cggagaggca attatttcag cgaaccttta aggaactgtt gcagacgagg
    16381 gctttgcggg ttgccaaata actgctccgg cgcgccttct tcctctattt tcccctgatg
    16441 gaggaaaatg acgtgagtag atacatggcg agcaaagccc atttcgtggg tcaccaccac
    16501 catcgttttt ccctcttccg ccagctgctg cataatgcgc agcacttcac ccaccagctc
    16561 tgggtcaagg gccgatgtcg gttcatcaaa caacagaact tccggttcca ttgccagcgc
    16621 ccgcgctatg gaaacacgct gttgctgacc accggacagg tgcaccgggt atttcccctg
    16681 cgcacgttcg tctatcccga cttttgccag atacttcacc gcccgctcgc gcgcttcctg
    16741 cttgctcagg cccaacacct gaatcggcgc ttccatgacg ttttccagca ccgtcatatg
    16801 gctccagaga ttgaagtgct ggaataccat cgtcaggcgt gtgcgcagta agcgcagttg
    16861 atttttatcg gcgactttga gttgaccgtc tttgtcgcgc accagattga tcgtctggcc
    16921 attgaccacg atcgaccctt cactcggttt ttcgaggaag ttaatgcagc gcagaaaggt
    16981 acttttcccc gatcccgacg atccgatgat gcttattaca tctccggcat tcgcttgcag
    17041 tgacacccct ttcagcactt cgtgttcgcc atagcgtttg tgcaaatcga taacgtttaa
    17101 tttattctcg gacatcgtat tctcagtgcg ttgaagaagg tttcacatgc tgcaaccagc
    17161 gtttttccgc tctgcgaaag aggctgatca ggacataaga gatgattaaa tagagcaccg
    17221 cggcaatgcc gaaggcggta aaaggttgat acgtggcggc gttaatatcg cgggctattt
    17281 tcagcagatc cggcaccgtg gcagtaaatg ccaacgcagt agagtgcagc atcaggatca
    17341 cttcgttgct gtatgccggt aacgcaatac gcagcgcaga aggcaaaata atgcagcgat
    17401 acattttaaa agtcgagaag ccataggctc tggcggcttc aatttcccca tgcggcaccg
    17461 aacggattgc cccagcaaaa atctcggtag tgtaagcgca ggtgttaagc gtcagcgcca
    17521 gcacggtaca gttcaggcca ctgcggaaaa aggcgttaag aaactcggtc cccttaacaa
    17581 tctccagcgt atacatgccg gaatagaaca ccagcaactg aacatacagc ggcgtaccgc
    17641 gaaaaatata ggtaaataac cagattggaa actggatgta tttattactg gagacacgac
    17701 caatcgccag aaacagcgcc aggactccgc ctatcactac cgacaaaata agcagccaca
    17761 gagtgatcgc cacaccagta aagcgataac cgtcggtcca cagcagcggt ttccagtatt
    17821 catgtaagat ttcgatcaca ggtcagccct cttcacaccc acagagtagc ggcgctcaag
    17881 gaacagcagc acaccattgg aaacggtggt gaaaaccagg taaatcacgc cacagacgat
    17941 agcgaaatag aacggttccc aggtactttt gcctgccagc tgcgtggctt tgaccacatc
    18001 ttccaggccg agtaacgaaa ccagtgcggt agatttgagg atcacctgcc agttgttgcc
    18061 aatgcctggc agggcgtaac gcatcatcgc cggaaacatg atccgccgaa acacttgccc
    18121 acgagtaaaa ccgaacgccc tcgccgcctc tatatgtcct ttcggcactg ccataaaagc
    18181 accacggaac gtttcggtaa aataagcacc gtagataaaa ccgagagtga taataccagc
    18241 gaccatcgga tcgatatcaa tctgcccgac gcccatcgcc tccgtcaccg tgtttagcgc
    18301 aatctgcaaa ccgtagaaaa tcagcagcat caacactaag tccggcacgc cacgaatcag
    18361 cgtggtgtac ccttcgaaaa tcagccccga aagccgattt tgcgagagct taccgccagc
    18421 gccaattaaa ccgatgatta cagcgagcac tacagagctg atagccagct ccagcgtgac
    18481 gagcgcaccc tgtaaaataa cacctgaaaa cccatacaac atgctgcctg tcctgtcgtg
    18541 tgtggtgaat taccgcctct tgtctccctc cggttacccg aagggagcgg gctttctttc
    18601 accgatggcg attaaccacc ataaacatca aaatcgaagt actttttcgc taatttctcg
    18661 taagtaccgt cagcgcgcat ttcggcaaag gctttgttca gtgcctcgcg cagttcgtta
    18721 tcttctttac gcaggcccat gccggtccct acgccaaaca gtttttcatc tttaacagac
    18781 gggccaccga atttgtaatc tttaccgaca ggttgtttga ggaaaccttc gctggcagcg
    18841 acctcatcct ggaacgcggc atcaatacgt ccggcagtca ggtcagaata aatgttgtcc
    18901 tgcccctgat acgagacgat ttcaatgcct tttggtgccc aatgttcatt accgaacgtc
    18961 tcctgggtag tgccctgcaa tacgcctacc cgtttgcctt tcagcgactc gactgtcggc
    19021 tgaatgtcag aatttttcgc caccaccaga cgagaatcgg cagcgtacag tttgtcggtg
    19081 aaggctattt cttgctgacg tttttccgta atggaaagcg atgacatgat ggcgtcaatc
    19141 ttcttcgctt ttaaggacgg gattaacgca tccagcggat tttcgacaaa cgtacattgc
    19201 gtattgatgc gtttgcataa ttcctttgcc agatcgatat cgaagccaac cagttcgcct
    19261 tgtgaatttt ttgattcaaa tggcgcatag gtcgggtcgg taccgatgcg gatgttttgc
    19321 ggaatcgcag caaatgccgc agttgcgctg gagaaggcca gaaccagaga gagcgatagc
    19381 accagttttt tcataatgtc ctcaactgac agtcttttta tagggaatat ttacaggttt
    19441 gttacactta tcgtgccata aaattgacca tcaaggcaaa atatcccgcc ccgttagacg
    19501 tattattttt cattaaaaat gcttaagtat gcatttcaat gcaccacgat agtgcaacaa
    19561 acctggcgtg caccatgatg atcattccat caggtacagc ttccctgcga cgtatcagtc
    19621 accgtagaca ttaaagtcaa aatacttttt cgccatcttg tcgtaggtgc cgtcctgacg
    19681 cagctcgcca agcgccttat tgaaggcagc cgtcagttca gcatcatctt tacgtagccc
    19741 tacaccggtg ccatcaccga agtatttttt gtcttttact gatgagccag caaaggcgaa
    19801 atctttacca gcaggttgct tgaggaatcc ttcgctggca gcaacttcat cctgcagtgc
    19861 agcatccaga cgtcctgcag ccagatcgga atagaccaaa tcctggttgg cataggccac
    19921 cacatccaca cctttgctac gccaggtctc gttagcgtaa gcttcctggg ttgatccctg
    19981 cagcacacca acatgtttac ctttcagtga gtccagcgtt ggctgaatcg gcgaaccttt
    20041 ggcggcaatc agacgggaat ctgcggcgta cagcttgtcg gagaaggcaa tctcctgctg
    20101 acgtttatcg gtaatggaaa gcgacgaaat aatggcgtcg atttttttcg ctttcagtga
    20161 ggggatcagc gcgtcaaagt cactggcaac ccaggtacat ttcacctgca tccgtttgca
    20221 catctcgtta ccgagatcga tatcaaagcc gacaaagtca cctttggcat ctttcgacga
    20281 gaacggtgca taggtggtat cggttccgat acgtaccgtc tccggcaatg ccgcatagct
    20341 ggaagccgct gcggaaatac cgactaacaa agacagagcg agaatcgact tcttcataca
    20401 taaccctcaa gtgaaaatgg cttttttatg ttgtgtattg tgttgtgtgt ttgcaggctt
    20461 gttcatgcag gtcttatgcc atcttgacga caacagtaac attcaacgtt aaatatgtta
    20521 ataagacgtt gcattattgt cctaaagttg aagatagcag gtatagcggt tgaatcgcag
    20581 cgtttcgatt gaatggcaga aacaaaatgt cgaggatttg atcgcggttg caaaattgcc
    20641 ctgaaacagg gcaacagcgg agttatgcgc cctgccagcg ggcaaagaga tcttcaggaa
    20701 gggttatcgc aaactggtca agaacacgat taaccgtctg atttatcaca tcatcaaggg
    20761 attgcgggcg atgataaaac gccggaacgg gaggcataat caccgcaccg atttctgctg
    20821 cctgcgtcat taaacgcaga tggcctaagt gcaatggtgt ttcacgcacg cagagcacca
    20881 gtgggcgacg ctctttcagc accacatctg ccgcacgggt cagtaagcca tcagtatagc
    20941 tatggacaat gccggacagg gttttgattg aacagggtaa aatcaccatc cccagtgtct
    21001 ggaaagaacc ggaagagatg ctggcggcaa tatcgcgcgc atcatgcgtg acatcagcta
    21061 atgcctgcac ttcgcgcagg gaaaaatccg tttcgaggga taaggtctgg cgcgccgcct
    21121 ggctcattac cagatgcgtt tcgatatctg tgacatcgcg cagaacctgt aataagcgca
    21181 caccataaat cgcgccgctg gcaccgctga tgcccacaat aagtcgtttc ataaatagtt
    21241 gccctgtcag acttgcaggc agactttgca ggatttcggc gggagttgca agtcagggtg
    21301 ccagaccggc accctgagcg aaggcatcat ccttcgttat gcatttcgag attttccact
    21361 tcgttctgac gttgcactgc tttggcgtcg tcattacgta aagtatcgag gaaatcgagg
    21421 tagccctgat caacatcttt ggtgacgtag acgccgttga acaccgagca ttcaaactgc
    21481 tggatatccg ggttttcagc gcgaacggct tcgatcagat cgttcagatc ctggaaaatc
    21541 aacccgtcag caccgatgat ctggcgaatt tcatctactt cgcgaccgtg agcgatcagt
    21601 tccgtggcgc tcggcatatc aataccataa acgttcggga agcgaatttc cggtgccgca
    21661 gaagcgaggt acactttctt cgctccggct tcgcgtgcca tctcgataat ctgctcagaa
    21721 gtggtgccac ggacgataga gtcgtcgacc agcaggacgt ttttatcgcg gaactcggcg
    21781 cggttggcgt tcagtttacg gcgcacggac ttacgacgca gctgctgacc cggcatgatg
    21841 aaggtgcggc caacatagcg gtttttaacg aagccctggc ggtacggttt gcccagaata
    21901 cgagcaattt ccagcgcgat atcacacgag gtttccggga tcgggatcac cacgtcgata
    21961 tccagatctt cccattcgcg ggcaattttc tcgcccagct tcgtgcccat attcacacgc
    22021 gcgctgtaaa cggaaatttt gtcgatgaac gagtccgggc gagcaaagta tacatactca
    22081 aacaggcacg gattgctgac cggattgtca gcacattgac gggtaaacaa ctgcccttct
    22141 tcagtgatgt aaatcgcttc gccaggcgcg acgtcacgca ggaaatcaaa gcccagtgta
    22201 tcgagcgcaa cgctttcgga agcgaccata tattctgtgc ggttctcgtc aatatcacgt
    22261 tttcccagaa ccagcggacg aataccgttt ggatcgcgga aagcaaccat accgtggccg
    22321 ataatcatcg ccacacaggc atacgcgccg cggattaagc ggtttgtagc agcgatggcg
    22381 gcgaaaatgt tgtcggcttc aagcgggtag tggcggaagt tgtccagctc gctggcgaag
    22441 atattaagca gaatttccga gtcggaagtg gtgttgatgt ggcggcgttt ttcttcaaac
    22501 agttttttac gcaactcgtg agcgttggtc agattgccgt tgtgggcaag cgtaatgcca
    22561 tacggagagt taacgtaaaa cggctgcgct tcagaggcgc tggagctgcc agccgtaggg
    22621 taacgcacat gaccaatgcc catattgccc tgcaaacgct gcatatggcg agcttcaaat
    22681 acatcgctca ccagcccgtt cgctttacgc aaacggaagc agttattggc atctatggtg
    22741 atgatgccgg cggcatcctg accgcgatgc tgaagcaccg ttaaggcatc ataaatcgac
    22801 tggttaaccg gcataacacc ggcgataccg acaataccgc acatacgtct ttttcctcgt
    22861 taagccacat ctcagagcac ttacgctctg ggcaagaaac ttgacgagct ttgcagataa
    22921 tcaaaaaacc atctgatgat aaaactgaac tgcggaatca gttgtgattt actccagtct
    22981 tcgcttttcg acaccccggt aaaggagtcg agaaagaaga gaatagcagc aacaatcaac
    23041 acaccgcgca atgcaccgaa acagacgccc agcacccgat cggtgcctga caaccccgtt
    23101 ttctccacca gcaggcctat cacgaagttc acgatagcgc caacgatcag ggtggcgata
    23161 aacaggaccg cgatggcaat cccatttcga accagttcgt cttcaaagcc cgtaaaccag
    23221 actgacaggt aagtgtagta atgactggca acaaagaaag cacaacccca tgtcaccagc
    23281 gataacgctt cacgaacaaa gccgcggatc aggctaacca gagaggaaaa agcaatcacc
    23341 gcgattatgg cgtaatcaat ccagaccata tgtgtcccac gattttacgc cctgtcatcc
    23401 tgttcggggc gcattctaac agaaaaagaa aacgtttgcg tagggatttc cttcccgcgc
    23461 atcaataaaa atggcgctga aaaaatattc aacgccatcg actttttatg cctttgcggc
    23521 atcggacaat gcgtgtcgga tgcggcgtaa acgtcttatc cgacctacgg ttctacccct
    23581 gcgtaggcct gataagacgc accagcgtcg catcaggcaa cggctgtcgg atgcggcgtg
    23641 aacgccttat ccgacctacg gttctacccc tgcgtaggcc tgataagacg caccagcgtc
    23701 gcatcaggca acgcctgtcg gatgcggcgt gaatgcctta tccgacctac ggttctaccc
    23761 ctgcgtaggc ctgataagac gcgccagcgt cgcatcaggc aacggctgtc ggatgcggcg
    23821 taaacgcctt atccgaccta cggttctacc cctgcgtagg cctgataaga cgcgccagcg
    23881 tcgcatcagg caagaccgta ttaattcggc gtatagccca ttaccacgcc acttaagcca
    23941 gaaagttgct tcaactcacc cagcgaacct ttcagcttat ctttcgaggc atccggccca
    24001 accagaatac gggtaatttt accctgcact ggcgtggatg gcgacgtata aacccgataa
    24061 ccggcaccgc gcagcttacc gacaatctca ttcactttat cggcattttt cagcgcgccc
    24121 agttgcacaa cataggcttt acccgttggc gctgcttttt cttccacgac cggcttcggt
    24181 tctggcttcg gcgcaggtgg tgcttcaacc ttcggcttag gcggctccac cggtttcggc
    24241 ttcggtggga cgaccggtgc aggttccggt tcaaactcgg tgttattggc tgcaatagtg
    24301 gccggatcga gcgacggtgc tgcggcatca cctgcccgca cctcttccgc tgcgccttcc
    24361 ggcggttgcg tcggcaacgc ctgggtggcg gctggcatca tatcaggctc atcgcgatcg
    24421 cccgcttttg gcaccagcgg gatagccgca aactcatcct gataatgttt tttctgcccg
    24481 tccagcagcc ctggaagtac aatcaccccc agcgccacca gcacgatcgt gcccactaac
    24541 cgattctgaa acttacttgc caccgcttct cctcgcgtca atcacttcca tgacatgtgc
    24601 gaccgtgtgg aacgaaccac acaccagcac ggtgtcttcc gctttagcgt ccgccattgc
    24661 ggcatcccat gcctgcgcaa cgctatcaaa tgatttgccg ttacccaaat gctcaagcag
    24721 ttgttctgcc gtggcaccgc gcggcccttc cagtggcgca caataccagt catcaaccac
    24781 gcttttcaac caggccagag ttccggcaat atctttatca tgtagcatac cgataaccgc
    24841 cagcacgcgc ccgtttttcg gtagcgtttt catacgcccg gtgagatatt ccgccgcatg
    24901 tggattatgc gcgacatcaa aaataacgcg tggcgactcg ctcacaatct ggaaacgtcc
    24961 cggcaaaatt gcgctggcaa tcccgtcgcg aatggcattt tcactgacct ccagcccgct
    25021 ggcacgcagt gccgccagcg ccgttgcggc attcggttgc gggacaagcg gcaacggcag
    25081 gttttccagc gtgccgtgag catcgctaaa cgcccagtca tgatcggtga cggaatagtt
    25141 ccactcaacg ccccgacgtt gtaacagtgc acctttttcc tgcgccacat cagcaatggt
    25201 agaaggcatt tccggctcac cgacaattgc cggtttttcg ctgcgaaaga tgcctgcttt
    25261 ctcgcgacca atactttcgc gatctggccc cagccagtcg gtatgatcca gcgcaatact
    25321 ggttactacc gcgacatcgg cgtcgacaat attggttgcg tccagacgac cgcccagccc
    25381 tacttccaga atcaccacgt caagttgtgc ctgtttgaac agccacaacg ccgacagcgt
    25441 accgtactcg aaataggtca gggaaatatc accgcgtgcc gattcaatct ccgcaaaaga
    25501 ggcggtgtgg gccgattccg gcaattcctg accctgcaca cgtacgcgct cggtataaca
    25561 caccagatga ggcgaactgt agacgcccac tttgtaccct gccgccatca gaatcgactc
    25621 cagcgtacgg caggtggtgc ctttgccatt cgtacccgca acggtaaaca caaatggcgc
    25681 tggtttcaga acgccaagac gcgccgcgac ctggctcacg cgctcaaggc cgagatcgat
    25741 agttttactg tgcaggtttt ccagataaga aagccacgaa gccagaggcg acgcggcttg
    25801 aggagtgcgt ttgataatca tggtatccgc tgattcgtta cggtgagaat agcaaaaggg
    25861 cagagccagt ggccctgccc ttatcagtta tcaggcctca ggttcctgat ccggtaccgg
    25921 gggtaccact acgccttcac gcggcgcttc aggattcggc gctggcagat tcatcaactt
    25981 cgccagaatg ctcgccagtt tcaggcgcat ttccggacga cggacgatca tgtcgatcgc
    26041 gcctttctcg atcaggaatt cactgcgctg gaatcctggc ggcagttttt cgcgaacggt
    26101 ctgttcgata acacgcggac cggcaaagcc gattaacgct ttcggttcag cgatgttgag
    26161 atcgcccagc atggcgaaac ttgcagaaac accgcccatc gtcgggtcgg tcagcacgga
    26221 gatgtacggc aagccgcgct cctgcatttt tgccagtgcc gcagaggttt tcgccatctg
    26281 catcagcgac atcagtgctt cctgcatacg tgcgccacca gaggcggaga agcagatcag
    26341 cgggcagtta tcttccagcg cctgctcaac ggcacgcacg aaacgtgcac ccacaacaga
    26401 ccccattgaa ccgcccataa aggcgaactc gaatgccgca gcgacaaccg gcattccata
    26461 cagagtgcct ttcatcacca ccagcgcatc tttttcgccg gtttctttct gcgcagatgc
    26521 cagacggtct ttatacttct tggagtcacg aaacttcagc acatctttcg gctcaagctc
    26581 gctacccagc tccacaaggc ttccttcatc taacaggcta tgcaggcgat tacgcgctgt
    26641 catacgcatg tgatggtcac acttcggaca gacctcaaga ttacgttcca gctcagcgcg
    26701 gtataaaacc tgaccgcagc tatcacactt agtccacacc ccttcaggaa tgctcgcctt
    26761 gcgggtggga gtaatgttgc ttttaattcg ttcaatccag ctcattaggg acctttctgt
    26821 ctgaacctgg ttcgatgcca gttttatctt tggggacgca taatgccatt tttgccccca
    26881 acagaccatg aatgttgcac attaaaacat aacagcccga aactttggat aaaaaagtgg
    26941 tcgaaccgcg gagttacttt ttattttgcg gcgcgtgccg cagcgcgttt gtgacggatt
    27001 atttcgatga cgcccggcaa aatagaaacc acaataatcc cgacgatcag cagcttaagg
    27061 ttatcctgaa ccatcgggat tgtaccgaag aaatagcctg cgtaggtaaa aagcagtacc
    27121 cacaacagtg cgccgatcac gttataagca gcgaaatgac ggtacgacat gtggcccatt
    27181 cccgcaacaa acggggcgaa cgttctgacg atcggcacaa aacgggcgag aataatcgtt
    27241 ttgccgccat gtttctcata aaactgatgg gttttatcga gataactgcg acggaaaatt
    27301 ttcgaattgg gattactgaa taacttttca ccgaacaagc gcccgatagt gtaattgacc
    27361 gcgtcaccca caatcgcggc gatcagcatc agcaccacca tcatatggac gttgagatcg
    27421 ttggtttcca gtgatgccaa cgctccggca acaaacagca atgaatcacc cggtaaaaac
    27481 ggcgttacca ccagaccggt ttcacagaac aaaattaaga acaaaatggc ataaacccag
    27541 acgccgtact ccgcgaccag ctctgccaga tgcacgtcaa tgtgcaggat aaaatcgatg
    27601 aggaaataaa tcaggtccat atttgcctat gccttgtact cgtcatcttt caggctgtaa
    27661 ctacgttggc tacagcctga aatgttccga gcattattct cgttagtccg ccagaaatag
    27721 cgggcccatt ggcggttttg gaagatcata ccggtcaggg taatccaccg cgaccagata
    27781 cagcccttcc gcttttgccg ttgctgccgc cagcgttctg tcctttgccg ccagcaactc
    27841 tgctatccag ctctccggct ggttgtgggc accgacttcc atcaggctgc cgacaatatt
    27901 cctgaccata tgatgtacaa aggcattcgc tttgatatca accaccacat aaggaccgtg
    27961 acgcgtgaca ttaatatgca taacgttgcg ccacggggtt cgggactggc actgcaccgc
    28021 acggaacgag gtgaaatcat tctcgcccag caagcactgc gcagcccgat gcatccgttc
    28081 agcgtccagc ggttcgtaaa aatgggttac ccctttactc agtaccgccg ggcgcagccg
    28141 atgattgtag atgatgtagc gataacggcg agccgtggcg ctaaatcggg catgaaaatc
    28201 atcaggtaca gctttaaccc aacgcacggc gatgtcacca ggtaaattcg catttacgcc
    28261 caacgtccac gcagcgtctt tgcgctgcgc ggtagtttcg aaatgcacaa cctgcccggt
    28321 accgtgtacc cctgcgtcag tacgcccggc gcagaagacg gtgatgggtt cgttcgccac
    28381 ctgggagagc gccttttcca gcttctcctg cacactgcgg acttcattct gccgttgcca
    28441 gccgtaatac ttactgccgt cgtactcaat gcccagcgca attttataaa ctggcggttg
    28501 ttgctggtcg gacattagta cagatactcc tgcaccagtt tctcggcgat ttttactgcc
    28561 atcagcgcgc cgccaaagcg aacgttatcg gccaccgacc agaactggac ttgctccggc
    28621 ataccgtagt cattacgcac gcagccaaca gaaagatgcg gcgtacccga agcatcacct
    28681 acctgagttg ggaattcgtt ctcttcagag agcacaatat cttccccttg aacaaacgca
    28741 tcacgcgctt cttctgctgc cagtggacgc agagcttcaa agttgaccat ctgggcatga
    28801 ccgtagaata ccggtgcctg gacgacgctt gccgaaatca tcagtccttc gtcctgcagg
    28861 attttgcgta cttcgtcaac gatacgacgt tcttcacgca cgctaccttc gctatccggc
    28921 agtaacggca gcatgttgaa cgcgagctga cgcccgaaga aatcttcttc gtcaatcgga
    28981 atgccgttga gcaatttcgc actctgcccc gctaacgcat cgaccgcttt tttgccctgg
    29041 gcggaggctg aaatcaggct ggtaacactg atacgcgaca aaccgccctg atcgattaac
    29101 ggtttcagtg ccgccagcag ctggctggtc agactgtctg gtacggcgat gacattccgg
    29161 ttgcggtaat cggtcagtac aaacgggttt acttccggca ccaccagcgg tacgtcgggt
    29221 tcaagagcaa acaatccgct gctgtcgatc accaggcaac ctgagttggt cgcttcttca
    29281 acccaggctg cggtagcttc tttgcctgcg acaaaaaatg ccagctgcgc ctgcgtccag
    29341 tcgaattcag cagcatcctg cacggtgatt gtcttaccac caaagcgcaa ttgttcgcct
    29401 gcgctttcgt tacgtgccag tgcataaatt tccccaaccg ggaactgacg ttcagccagc
    29461 gtttcaagca gggcttcgcc cacagcgcca gttgtgccca ggacggcaat gttccagcct
    29521 tcagacatgg tggtttactc cagaaatagc aaagctccct gccaatgtta tagcagagag
    29581 catgaagaag agattaacgt gccggatgat gaacggcgtt aaaacccagt ttacacagca
    29641 atgatgccgc actggcgtca tcacaaatta catacagaga tgaccattca cggcgctcaa
    29701 gatagttttt gcgcagttta tcgaactcac ccggtatccc ggcgacttta cgcagcggtg
    29761 cgtcatcgcg gcgcacatca tacaccaaat gcaccagcct tttcagcgtc ggttgatcga
    29821 gcggtccatg cagagtaatg cgaccaaact ctggcgcagg cagtaatgta tccagcgcaa
    29881 cgtgctgttc atgcccaata aacttgctat aggcttcaaa cacttgcgta gtaccgcgtg
    29941 ctttaccttc cagggtatag cctgcaatat gcggcgtgcc gatatccact tttgtcagca
    30001 attccacatt tagttccggt tcgccttccc agacatccag cactacgctt aacttctggc
    30061 cttcattcag gcaggtcagc agcgcggtat tatcgacgac tgcgccacgg caggcgttaa
    30121 tcagaatcgc tccgggcttc aggctacgga tcagtttttc atccgccaga tgcagcgttt
    30181 tgtacggccc gtctttgaag agtggcgtat ggaaagtcag aatatccgcg cgctggacta
    30241 actcatccag cgagcggaaa tcaccctcat ccccacagtc ggcgcgaggc ggatcgcaaa
    30301 gtaaggtttt aatccctaac gcttccagtc gtgcctgtaa acgccgacca acgttaccaa
    30361 cgcctacgat ccccacggta cgctcgtgca gtgaaaatcc atcgcgttcg gcaagcatca
    30421 gcagggagga gaaaacatat tccaccaccg caatcgcatt acagccaggt gcagcggaaa
    30481 aaccaattcc cgcctgcttc aaccatgctt catcgacatg gtcggtcccc gcagtggcag
    30541 tgccaacaaa tttaatgggt tttcctgcca gcaaagattc attcactttc gtgaccgaac
    30601 gcaccatcag cgcatccgcg tctgccagtt gagcgacggg gattggacgc ccgggaaccg
    30661 cgatcacctc acccaaacgg ctaaataagt cgcgggcata aggcatattt tcatcaacaa
    30721 ggattttcac gtttgtgtta cctgtatgag acgagagtta accggacaag tgtgccataa
    30781 tctcgcggcc aggcatactt gcgaagattt caggtataag gatacgtaat gatacaacct
    30841 atttccggcc ctcctcctgg gcaaccacca ggtcagggag ataacctgcc gtctggcgcg
    30901 ggcaatcagc ctttatccag tcagcaacgt acttcgctgg aaagcttaat gacgaaagtg
    30961 acctcactga cgcaacagca aagagcagaa ctgtgggcgg gtatcaggca cgatattggt
    31021 ctgtcgggag attcaccgct gctttcgcgt cactgccctg ccgctgagca taatctggcg
    31081 caacgtctgc tggccgcgca aaaaagccat tctgcccgcc agcttttagc gcaattaggg
    31141 gagtatttac gtctggggaa taatcgtcag gcggtcacgg attatatccg tcataacttt
    31201 ggtcagacgc cgctgaatca gctctcaccg gagcaattaa aaaccattct caccctgttg
    31261 caggaaggga agatggttat tccgcaaccg cagcagagcg aggcgaccga tcgtcctcta
    31321 ttaccggcgg agcacaatgc gctcaaacag ctggtgacca aacttgcggc ggcaacgggg
    31381 gagcccagca aacagatctg gcaatcgatg ctggaacttt ccggggtgaa agatggcgag
    31441 ttaattccag cgaaactgtt taaccatctg gtgacctggc tgcaggcgcg tcagacgcta
    31501 agccagcaaa atacgccgac gctggaatca ctacaaatga cgctaaaaca acctttagat
    31561 gccagtgaac tggcggcgtt atcggcatat atccagcaaa aatatggcct ttctgcgcaa
    31621 tcatcgcttt cttctgccca ggccgaggat attcttaatc agctttatca acggcgggtt
    31681 aaagggattg atccgcgtga tatgcaaccg ctgcttaatc cttttccacc gatgatggac
    31741 acgttgcaaa atatggcaac gcgtcccgcg ctgtggatac tgttagtcgc gattatcctg
    31801 atgctggtct ggctggttcg ttaaccccga cgaaacgaca gtatcgtgac aataattccc
    31861 agcaccgcag agatcgcccc ggcaagaaat accgaagagt aaccaaacgt ggtcgccagc
    31921 attcccgcaa gcggcccgga gacgccgagg gcgatatcct gaaacgcggc gtaaccgccc
    31981 agtgcggtgc cgcgaacttg tgaggggacg cgtttaacca cctccacacc cagcgcagga
    32041 aagataagcg aacatccggc tccggttaac gccgcgcccg ctaatgcgac ccacgcccct
    32101 ggggcttgcc agagcagcaa caagcccacc gtttctacaa gcagagagac aatcgccact
    32161 ttcacgccgc caaaacggtc cggcatccag ccaaacatga cgcgcatcac gacaaatgcg
    32221 ccgccaaacg cggtaagagt aaagcccgcc atcgcccatc ctttgctggc aaagtagagc
    32281 gaaacgaaag tcccgataac cgcaaaacca acgccttgta gtgccagacc taaccctggt
    32341 ttccagataa gtccgacaac gctccacaga gatggacgtt ctcccgccag ggctggtact
    32401 ttgcgcactg tgccgttaca ggcccacgcc agtaagggta atgccattgt ggtgagcgcc
    32461 agtgcggcaa aaccgtaatg gctatgaatc aacaggccaa gcggagcacc aacagcgagg
    32521 gcaccgtaaa tcgccattcc attccatgac atcactttgc cagagtgttt tggccctacg
    32581 atgcctaacc cccaggtcag agcgcctgtc agtaactggc tttcaccaaa cccaagaatc
    32641 aaacgcccga tgaccaacag ggcaaatttg aacggtgcgg agacaggcaa aatcgccgcc
    32701 agcagcaacg cgccgccagc cagaccacaa gctaacatcc cctgaagcgc cgaacgtttt
    32761 gcgccatatt gatcggccag tcgcccggca tagccacgcg tcagcaccgt agccagaaac
    32821 tgaatgccga cggcaatgcc gaccatggta ttgccatagc ccagatcatg atgaacaaac
    32881 aacgggataa ccggcaacgg caaccctacg gtcatgtagg tgagaaaaac cgcaaaagcg
    32941 atgcggaaga gcgaaaaatt ggcagaagat cgtgtttcgg tttggcttac agcagtcatg
    33001 cattactcca gaatgcagcg caaggcgagg agtatccccg tctcatctct ctggtttcag
    33061 ggttacagtg cgttggcagg atttaacgcg tacgtctttt cagaaggaaa tcgacaaagc
    33121 ggggagtttg cctggaactg gcggcgattg tcaatgatgt gaaaaaggga accatcaggt
    33181 tcccttttgc gttagtgccg gaggccgcat tggcgcgtaa cgtcggatgc gacgctggcg
    33241 cgtcttatcc gacctactcc ggcctactgc gaattaatct ttcagcttgc gcattaccag
    33301 cgtggcgttg gtgccgccga agccgaagct gttagacata acagtggtca gttcgcgatc
    33361 ggtcgtttcg gtcacgatgt tcagacccgc agcctgctcg tccagctctt caatgttgat
    33421 gctcggggcg ataaagccgt gttccagcat cagcagagag tagatagctt cctgtacgcc
    33481 agcagcgccc agagagtgac cggtcatggc tttggttgca gaaatcgccg ggctcttatc
    33541 gccgaacact tcacggatag ctgccagctc tttcacgtcg ccaaccggag tcgaagtgcc
    33601 gtgggagttc aggtaatcga ttggggtatc aacgccatgc atcgccatct gcatacaacg
    33661 tactgcgcct tcgccagagg gagcaaccat gtctgcgcca tcagacgttg cgccgtagcc
    33721 aacgatttca gcatagatgt gagcaccacg cgccagcgcg tgttccagct cttcaaccac
    33781 caccataccg ccgccgccag cgataacgaa accgtcacgg tgagcatcgt aagtacggga
    33841 ggctttttcc ggggtgtcgt tgtatttagt agacagcgca cccattgcgt cgaattcgca
    33901 agccatttcc cagcacagct cttcgccgcc gccagcaaac acgatgtcct gtttgcccag
    33961 ttggatctgc tctactgcgt taccgataca gtgtgcggaa gtcgcacacg cggagctgat
    34021 ggagtagtta acgccatgaa ttttaaacgg cgtggcgagg caggcagaaa cgccggatgc
    34081 catcgctttg gtgaccacat acgggccaac cgctttcagg ccgcgcgggc cgcgcattgc
    34141 gtcagcgccg aacacctgga aacgcgggga accgccgcca gaacctgcaa tcaggccaac
    34201 acgcggatta ttctggtaag cttccggaga gaggcccgca tctgcgattg cctgctccat
    34261 agaaaggaat gcataaatgg atgcgtcgct cataaagcgc acaactttgc ggtcaatgag
    34321 gccagtggta tccagtttta cgttgcccca gacgtggcta cgcatgccgg aatccttcag
    34381 ctcctgagag aaagtgatcc ctgaacgtcc ttcacgcaga gatgccagga cttcctgctg
    34441 gttattaccg atgctggaaa caatgcccag gccagtaatc actgcacgtt tcattcaata
    34501 cctctgtaag tcgcacatag agtaagtttc gaatgcacaa tagcgtacac ttgtacgccg
    34561 aacaagtccg atcagccaat taatagagaa atttgcgcag ccttacacac atcgctaaga
    34621 tcgagctacc gcctgtaaga cgagtaactt acgtgaaaca ctactccata caacctgcca
    34681 acctcgaatt taatgctgag ggtacacctg tttcccgaga ttttgacgat gtctattttt
    34741 ccaacgataa cgggctggaa gagacgcgtt atgtttttct gggaggtaac caattagagg
    34801 cacgctttcc tgaacatcca catcctctgt ttgtggtagc agagagcggc ttcggcaccg
    34861 gattaaactt cctgacgcta tggcaggcat ttgatcagtt tcgcgaagcg catccgcaag
    34921 cgcaattaca acgcttacat ttcattagtt ttgagaaatt tcccctcacc cgtgcggatt
    34981 tagccttagc gcatcaacac tggccggaac tggctccgtg ggcagaacaa cttcaggcgc
    35041 agtggccaat gcccttgccc ggttgccatc gtttattact tgatgaaggc cgcgtgacgc
    35101 tggatttatg gtttggcgat attaatgaac tgatcagcca actggacgat tctctaaatc
    35161 aaaaagtaga tgcctggttt ctggacggct ttgcgccagc gaaaaacccg gatatgtgga
    35221 cgcaaaatct gtttaacgcc atggcaaggt tggcgcgtcc aggcggcacg ctggcgacat
    35281 ttacgtctgc cggttttgtc cgccgcggtt tgcaggaagc cggattcacg atgcaaaaac
    35341 gtaagggctt tggacgcaaa cgggaaatgc tttgtggggt gatggaacag acattaccac
    35401 tcccctgctc cacgccgtgg tttaatcgta cgggcagcag caaacgggaa gtagcgatta
    35461 tcggcggtgg tattgccagc gcattgttgt cgctggcgct attgcgtcgc ggctggcaag
    35521 taacgcttta ttgcgcggat gaagcccccg cgctgggtgc ttccggcaat cgccaggggg
    35581 cgttgtatcc gttattaagc aaacacgatg aggcgctaaa ccgctttttc tctaatgggt
    35641 ttacttttgc tcgtcgactt tatgattcat tacccgttaa atttgatcat gactggtgcg
    35701 gcgtcacgca gttaggctgg gatgagaaaa gccagcataa aatcgcacag atgttgtcaa
    35761 tggatttacc cgaagaactg gctgtagccg ttgaggcaaa tgcggttgaa caaattacgg
    35821 gcgttacgac aaattgcagc ggtattactt atccgcaagg tggctggctg tgtccggcag
    35881 aactgacccg taatgtgctg gaactggcgc aacagcaggg tttgcagatt tattatcaat
    35941 atcagttaca ggatttatcc cgtaaggatg actgttggtt gttgacgttt gcaggagatc
    36001 agcaagcgac acacagtgta gtggtactgg cgaacgggca tcaaatcagc cgtttcagcc
    36061 aaacatcgtc tctgccagtg tattcggttg ccggtcaggt cagccatatt ccgacgacac
    36121 ccgaactggc aaaactgaaa caggtgctgt gctatgacgg ttatctcacg ccgcaaaatc
    36181 cggcgaatca gcaccattgt attggtgcca gttatcatcg cggcagcgaa gagacggcgt
    36241 acagtgaaga caatcagcag cagaatcgcc agcgattgat tgattgtttc ccacacgcac
    36301 aatgggcgaa gacggttgat gtcagtaaaa aagaggcgcg ctgcggtgtg cgttgtgcca
    36361 cccgcgatca tctgccaatg gtaggtaatg ttcccgatta tgaggcaaca ctcgtggaat
    36421 atgcgtcgct ggcggagcag aaagataagg cggtaagcgc gccggttttt gacgatctct
    36481 ttatgtttgc tgctttaggt tcgcgcggtt tgtgttctgc cccgctgtgt gccgagattc
    36541 tggcggcgca gatgagcgac gaaccgattc cgatggatgc cagtacgctg gcggcgttaa
    36601 atccgaatcg gttatgggtg cggaaattgt tgaaaggtaa agcggttaag gccgggtaat
    36661 ctgctctcgc agtgtttgcc ggatgcggcg tgaacgcctt atccgaccta cgtgtgacgt
    36721 gtaggcctga taagacgcga taagcgtcgc atcaggcatg gtgctccaga cgccgcaact
    36781 tactgctgtg acgcctgctg aaacaggttt tcccacatat cggtcaccag cgcctggtca
    36841 cgcggcgaca attcaccagc accaatggct ttttccagac tctggctaac ggtcgtatgc
    36901 accgcctgag cggagtggtc atcaccactt tccagttctg cgatggctaa tgtaaggtgg
    36961 ccacgcaaat acccactggc aaacaactca tcatcactgg catggtcaac cataccgtcg
    37021 attaatgcca gaatgcgtga ttcaaactcc gcgatcatct tctttcctca ttgtaaaacg
    37081 tggcgctggc ttcagttgag cgcttccggc cacgggaact gttccgccgt aagttccggc
    37141 gtgtgataat aattttgtag tgctttaatg aaacgtgccg ggcgttccgg aatgccattt
    37201 gttagataat ccatcacctg cgcatgtact cgccgttgaa acaccacgcg gtccggttcg
    37261 aagtcgcctt ccagattgtc gcaactgacg ttaaacgggt atcccgccgc cacgcagaac
    37321 aaccagtcga gcgcctgcgg cttcacttca acatcttcaa actggctttg cgtttgggca
    37381 tcgcgaccgt ccgggcaata ccagtagccg aaatcaacct gctcgcggcg cgctttcccg
    37441 gcaatacacc agtgcgaaat ctcatgaatt gcgctggcat aaaagccgtg ggcaaagacg
    37501 attcggttat acggtacttc cgcatcagcg ggaagataga tcggttcgtc gtcgccttta
    37561 atcagacggg tattaaaatc atcggcaaag cagctattaa aaatttcaat caactgctca
    37621 tagtggtgtg tactgttcat tagttcatcc ccaaccagtg gaggatctcc tgtccgtggc
    37681 tatcataaag taatttggca ctcatcaccg ccgagacgat aacgatcatc gggcggatca
    37741 gtttttgtcc tttgctcaac accagtcttg aacccatgcg cgcgccaagg aactgcccca
    37801 ccagcatcac aaagcccgtc gcccaaatca ctttgccgcc gagaataaac agcagtaaac
    37861 cgccgatgtt tgacgttgcg ttgagtaatt tggcgtgagc cgtggctttg gcgaggttga
    37921 atccgcacag cgtaacaaag gccagcgcgt aaaacgatcc ggctgccgga ccaaagaatc
    37981 cgtcataaaa accgacgcag ccaccggcaa ttaacgcgaa gggcaaaccg tacatccggc
    38041 gctggcgatc ttcttcaccc agctttggca tcagcaagaa atagaggccg atacaaatca
    38101 ccagaatggg caaaatctgc cgtaagacat cagcctgaac gtactgtacc aacagtgcgc
    38161 cgctcattga gccgacaaag gtcatggcaa tattaagttt ctgatcgctt aaactaacca
    38221 ctttgcggcg aataaagtag atagtggcgg aaatagagcc gccgcaggct tgcagtttat
    38281 tggttgccag agcattagcg ggagacatcc ccgctgccat caatgccgga atggtgagta
    38341 acccaccgcc accggcaatc gagtcgataa atcccgccag catggcgaca aaaaagagaa
    38401 ctcccagcaa cagcggggaa accataaaca ggctattaaa cgtttccatt agatcacgtg
    38461 ctcatccagt agcgcctggc aggaaggcgg caacggaggc ggtgtcttct tctcaggctt
    38521 tgttgttccc ggttttggag gttcaaacca gctttgcagt tctgccccgc aaccatcgcc
    38581 tggtggcggt aaaggttgat cttcacactc cagactatcg gcaggacaac gtaatcgtac
    38641 atgcatatgc gcacgatgct ggaaccaggg tcgcactttg cgcaaccagt cgcgatcggt
    38701 gcccgcatca aggcaaagtt gttgtttaat cgccggatta acaaaaatgc gcgtgacgtc
    38761 tttgtcctgg gcggcgagtt tgatcaagct gaaaatttct ggcttccaca gcgtggagac
    38821 aacgtgttta ccgtcgcggg aaactaagtc cagtgcttgc gggcgcaaga gctgcgcgga
    38881 ggtccggcgt gttttcggca gttgcagaaa gatatcgaca tccagtccgg tctggtggct
    38941 ggcgtgaccg ccgttgaaac gcccaccagc aggcattccc atatcgccaa tcaacaccgt
    39001 acccatgccc agattgctca cctgactact caggcgctgg ataaacatca ccaggtccgg
    39061 gtgaccgaaa tagcgacgct gatcggtacg catgacctga taatgttcgg actgtatcgg
    39121 caacgtgtca gcgccgacga tacaaccatt ggaaaaactg cctatcgatt gtgcgctacc
    39181 cggcacaggt tgggttattt tttgccacgg cgtcgctgcc aggctgacgc tactggcaag
    39241 cagggccagc agcgcaatcg cggttttgtt cattttttac cagcgtggaa tatcagtctt
    39301 cacatcggca ttttgcgccc gctgccgtaa caggtgatcc attaaaacga tcgccagcat
    39361 cgcttctgct atcggcaccg cgcggatccc gacacaggga tcgtgacggc ctttggtgat
    39421 catctcaact tcttcgccaa agcggttaat ggtgcgcccc ggcacggtaa tactggaggt
    39481 cggtttcagt gccatatgag caatgatttg ctgcccgctg ctaattccgc cgagaatgcc
    39541 gcccgcatgg ttactctgga aaccgtcttt ggtgatttcg tcgcggttct ggctgccacg
    39601 cagcgccacc acgtcaaaac catcaccaat ttccacgcct ttcaccgcgt taatgctcat
    39661 tagcgcatgg gcgatgtcgg catccaggcg atcaaagacc ggctcaccaa gcccggcagg
    39721 aacgccactg gcaacaacgg tgactttcgc gccaatagag tcgccctctt ttttcagcgc
    39781 gcgcatcagt tcatctaacg cgtcgatttt gtccgggtcc gggcagaaga atggattttg
    39841 ctcgacctgc gaccagtctt tgatttccag cggaatgtcg cccatctggg tcaggcagcc
    39901 gcgaatttcg atgccaaatt tctcagcgag atattttttg gcaatcgccc ctgctgcaac
    39961 gcgcatggcg gtttcacggg cggaagaacg tccaccgccg cgataatcgc gcagaccgta
    40021 tttttgttcg taggtgtaat cggcatggcc aggacggaaa acgtccttaa tcgcactata
    40081 atcctgagaa cgctgatcgg tattttcgat caacaaacca atgctggtgc cggtagtaac
    40141 accttcaaaa acaccggaga gaattttgac ctgatctggc tcacgacgct gggtggtata
    40201 gcgcgatgtc ccagggcgac gacggtcgag gtcatgttgc aggtccgctt ccgtcagcgg
    40261 aatgcctggc ggaacaccat cgacgatgca gccgagcgcc agcccgtgcg attcgccgaa
    40321 ggtggttacg cgaaagagtt gtccaattgt gtttccagcc atcacggctc cgttattgtt
    40381 gtgtttgcgt gtttacttaa tctttataaa tcgcgaaatg ttcacgggcc gccagcagct
    40441 gcgctttggt gagcataaac acaccatcgc cgccgttatc aaactccagc caggtgaacg
    40501 gaacatcggg atattgttcc ataagatgta ccatgctgtt gccgacttca caaatcagta
    40561 cgccatcatc agcaagataa tctgccgcgt ttccgagaat gcgacgcgtc agtttcaggc
    40621 cgtcggtgcc agatgccagg cccagttccg gctcgtggcg gtattcattt ggcaggtcgg
    40681 acatatcttc cgcatcgaca tacggcgggt tagtgacaat caggtcgtac tgcactttcg
    40741 gcaaatcacg gaacagatcg gaacgaatcg gtatgacgtt gtggatcaga ccgtgttctt
    40801 cgatgttctg ttcggcaacc gccagcgcgt cgggagagat atctaccgca tcgacttccg
    40861 cttccgggaa ggcataagca caggcaatgg cgatgcagcc gctaccggta cacatatcta
    40921 aaatatgctg cggttgcttg ctgataagtc cggcaaattt attgttgatc aattcaccaa
    40981 tcggcgagcg cggcaccagc acgcgttcat cgacgtaaaa ttcatggccg cagaaccacg
    41041 ctttgttggt caggtaagct accggaatgc gttcgttgac gcggcggatc acgcgctcaa
    41101 caatacgatg tttttcgctg gaggtcagac gcgcggtgcg catatcttcc ggaatatcca
    41161 gcggcaggta gagcgacggc aataccagct gtacggcttc atcccacggg ttatcggtgc
    41221 cgtgaccgta ccagatattt gccgcgctga agcggctcac cgaccagcgc aacatgtcct
    41281 gaatggtttg cagctcattt actgcttcat caacgaaaat tttatccacg tattcctcca
    41341 gggcatgatc gcaataattt cggcggctag tttgccatga agatgacgat aaatcagcat
    41401 tcacgcgcgg tgagtgagga aaaatacgtt taaaacgatc gattgcgcta cgagtcgggt
    41461 aaactgtagg aaaattagaa atagagacag ataaatgaaa aagaaaacaa cacttagcga
    41521 ggaggaccag gctctgtttc gccagttgat ggcggggact cgcaagatta agcaggacac
    41581 cattgtccat cggccacagc gtaaaaaagt cagcgaagtg cccgtcaaac ggttgattca
    41641 ggagcaggtt gacgccagtc actatttctc tgacgaattt caaccgttat taaatgctga
    41701 tggcccggtg aagtatgtac gccctggtgt agaccatttt gaggcgaaaa agctgcgccg
    41761 cggcgattat tcgccggaat tgtttttgga tttacacggt ctgacgcagc tgcaggccaa
    41821 gcaggaactg ggcgcgttga tcgccgcctg tcgccgcgaa catgtgtttt gcgcctgcgt
    41881 gatgcatggt cacgggaaac atatattgaa gcaacaaaca ccgctgtggc tggcacaaca
    41941 tccgcatgtc atggcgtttc accaggcacc aaaagagtat ggtggtgatg ccgcattgct
    42001 ggtgttgatt gaagtggaag agtggctacc gccggagttg ccatgaatat tagggggcgg
    42061 atgcgttttg ccatttcgcc ctgaatgctg gtatcgaatg ccggatgcgg cgtaaacgcc
    42121 tgatccggct tatcaaaatg tgcaaattca atacgttgtg attcagttgt aggcatgata
    42181 agacgcgtca gcgtcgcatc aggcatcaac gcacaactac agttcattca gtacatactg
    42241 gaaacggcat gtagaatcaa ttggctcacg gctactggta ttcacctgta ccacaatgtc
    42301 gtgtagcttt ctatccgtca atggttgacg tgagggggtg actgtcacct ttgcattatc
    42361 acaaccatac gaggtaaagg ttaataagtc agaaacagca cgacaatatg cccctctgga
    42421 atcaaaaata ctgccatcgc agagtttgcc gctttccatc aggtaggtcg aaagcgtagt
    42481 cgtgattgtc gaaccatcgg attgcaatcg cagcgcttta ttatggctaa cataattatc
    42541 actccaggat atatcaaacg aagcgacggg aattgtcggg caaatagaat catgaactgt
    42601 tgggcttgta tacgtaccaa atccaaatgc ctcagatact gaataaaatg aggcaatcca
    42661 cggacaacca taattatcag attctacagt gacgttaaaa gtcatatatg cacagacaaa
    42721 gacacgagta ttgataccag ggcaatttga ggtgtccgta cgtctataat cagtcaaaac
    42781 cagtgtcggt gccgggccag atccgctcca ggatattaac ttgagggtgt cgccttttct
    42841 tgcattggag ttgtattgtc cgtagcttac ttctcccggc gtcactaccg tgacatagaa
    42901 atataaactg ccggaagcag taacgttttt catcgcataa taaattttag tactggaaaa
    42961 gcttgcactc atgagtttat caccggcata gcactttggt gtttctaaac tgagagcaag
    43021 taatatcaga cttgtcaaaa atatcgtcca ttttttcatt ttaaaaatac tcaaaagtta
    43081 ccgtcattgt ggcggtgaaa tcacccgatg taacatttcg tccatttacg gtctgtaacc
    43141 aggcattaaa attgacactg ttatttccct gatttaatgg aaatgaggca cctgtggtac
    43201 tgttaatagg tacagccgcc cctccggcag tttcaatgcc aatcccaaca cccgttgcag
    43261 aagatgacga atcaatcgac agaaatcctg gtaagtcggt atcttctgtt cccatcaagg
    43321 tcaccttgac attaaacgcc gtggtgcttt tgcaatcttt caactgaaaa actaacggta
    43381 cgcgatctga ctgaccacgt tgcattaaat cgctggcagc aattgtgggg aaatgaattt
    43441 ctgcaagtaa gttgc
//
LOCUS       DBFIFM010000013        41846 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.013, whole genome shotgun sequence.
ACCESSION   DBFIFM010000013 DBFIFM010000000
VERSION     DBFIFM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.013"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..2657
                     /gene="cas3"
                     /locus_tag="N7C74_000670"
     CDS             <1..2657
                     /gene="cas3"
                     /locus_tag="N7C74_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HGU2430609.1"
                     /translation="TFFDFWGKTRRGEKEGGDGYHLLCWHSLDVAAMGYLMVKRNCFG
                     LADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLAPELKIPEGSRKNYEKI
                     SHSTLGYWLWNYYLSECEELLPSSSLSSRKLTRVIEMWMSITTGHHGRPPDRIDELDN
                     FLPEDKAAARDFLLEIKALFPLIEIPTFWDDDEGVELLKQLSWYISATVVLADWTGSS
                     TRFFPRVAHPMDIKDYWQKTLVQAQNALTVFPPKAETAPFTGINTLFPFIEHPTPLQQ
                     KVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLMAARKAQGLFFGLPTMATANAM
                     YDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFSQGCA
                     AWFANSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYMS
                     CILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQQEAPFLEKDDYPWLT
                     HVTKSDVNSHRVATRKDVERSVSVGWLHSEQESIARIESAVSQGKCIAWIRNSVDDAI
                     KVHRQLLARGVIPASSLSLFHSRFAFSDRQRIEMETLARFGKEDGSQRAGKVLICTQV
                     LEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILAP
                     VWDDAPGDEWFGSAMRNSAYVYPDHGRIWLTQRVLREQGAIQMPHAARLLIESVYGED
                     VVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFKPGYAANINDYLPEKLSTRLAEESVS
                     LWLATCIAGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRQWCIEQRQD
                     PEMANVILVTDDESCGYSAREGLIGKVD"
     gene            2755..4317
                     /gene="casA"
                     /locus_tag="N7C74_000671"
                     /gene_synonym="cse1"
     CDS             2755..4317
                     /gene="casA"
                     /locus_tag="N7C74_000671"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HGU2430610.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQSSFAPKDYRRWDDIWEDGLEAEKLREALLSLEHPFQFGPDSPSFM
                     QDFEVLMGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCSHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQAPLWRKLWLNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLERVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            4314..4850
                     /gene="casB"
                     /locus_tag="N7C74_000672"
                     /gene_synonym="cse2"
     CDS             4314..4850
                     /gene="casB"
                     /locus_tag="N7C74_000672"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HGU2430611.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAALSANVKAIDERLPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRVVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRHQRPTEFIRI
                     RWALEYYQAGDVDNEQNQ"
     gene            4862..5917
                     /gene="cas7e"
                     /locus_tag="N7C74_000673"
     CDS             4862..5917
                     /gene="cas7e"
                     /locus_tag="N7C74_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HGU2430612.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTLRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTEQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            5928..6674
                     /gene="cas5e"
                     /locus_tag="N7C74_000674"
     CDS             5928..6674
                     /gene="cas5e"
                     /locus_tag="N7C74_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HGU2430613.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSAPD
                     LLSAIISRRDYYTDAWWMVAVATTPDAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYQQYQDSFRELKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTINQGPWLSKEEPCTSQE"
     gene            6656..7306
                     /gene="cas6e"
                     /locus_tag="N7C74_000675"
     CDS             6656..7306
                     /gene="cas6e"
                     /locus_tag="N7C74_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HGU2430614.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRSFVPELRTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEV"
     gene            7303..8226
                     /gene="cas1e"
                     /locus_tag="N7C74_000676"
     CDS             7303..8226
                     /gene="cas1e"
                     /locus_tag="N7C74_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HGU2430615.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVDQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEKVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            8223..8516
                     /gene="cas2e"
                     /locus_tag="N7C74_000677"
     CDS             8223..8516
                     /gene="cas2e"
                     /locus_tag="N7C74_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HGU2430616.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     repeat_region   8612..8824
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=8612..8641
                     /rpt_unit_seq="tgtgttccccgcgccagcggggataaaccg"
     gene            complement(8906..9943)
                     /gene="iap"
                     /locus_tag="N7C74_000678"
     CDS             complement(8906..9943)
                     /gene="iap"
                     /locus_tag="N7C74_000678"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HGU2430617.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPQSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            10195..11103
                     /gene="cysD"
                     /locus_tag="N7C74_000679"
     CDS             10195..11103
                     /gene="cysD"
                     /locus_tag="N7C74_000679"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HGU2430618.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHQNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            11105..12532
                     /gene="cysN"
                     /locus_tag="N7C74_000680"
     CDS             11105..12532
                     /gene="cysN"
                     /locus_tag="N7C74_000680"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HGU2430619.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRSRVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGEK"
     gene            12532..13137
                     /gene="cysC"
                     /locus_tag="N7C74_000681"
     CDS             12532..13137
                     /gene="cysC"
                     /locus_tag="N7C74_000681"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HGU2430620.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            13187..13510
                     /gene="ygbE"
                     /locus_tag="N7C74_000682"
     CDS             13187..13510
                     /gene="ygbE"
                     /locus_tag="N7C74_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HGU2430621.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLMTVGIMFGALF
                     MWLLG"
     gene            13704..14015
                     /gene="ftsB"
                     /locus_tag="N7C74_000683"
     CDS             13704..14015
                     /gene="ftsB"
                     /locus_tag="N7C74_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HGU2430622.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            14034..14744
                     /gene="ispD"
                     /locus_tag="N7C74_000684"
     CDS             14034..14744
                     /gene="ispD"
                     /locus_tag="N7C74_000684"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HGU2430623.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVYA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGAAITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            14744..15223
                     /gene="ispF"
                     /locus_tag="N7C74_000685"
     CDS             14744..15223
                     /gene="ispF"
                     /locus_tag="N7C74_000685"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HGU2430624.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            15220..16269
                     /gene="truD"
                     /locus_tag="N7C74_000686"
     CDS             15220..16269
                     /gene="truD"
                     /locus_tag="N7C74_000686"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HGU2430625.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELVELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            16250..17011
                     /gene="surE"
                     /locus_tag="N7C74_000687"
     CDS             16250..17011
                     /gene="surE"
                     /locus_tag="N7C74_000687"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HGU2430626.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            17005..17631
                     /gene="pcm"
                     /locus_tag="N7C74_000688"
     CDS             17005..17631
                     /gene="pcm"
                     /locus_tag="N7C74_000688"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HGU2430627.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            17816..18646
                     /gene="nlpD"
                     /locus_tag="N7C74_000689"
                     /pseudo
     CDS             17816..18646
                     /gene="nlpD"
                     /locus_tag="N7C74_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
     gene            18638..19168
                     /locus_tag="N7C74_000690"
                     /pseudo
     CDS             18638..19168
                     /locus_tag="N7C74_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
     gene            19168..20595
                     /locus_tag="N7C74_000691"
     CDS             19168..20595
                     /locus_tag="N7C74_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HGU2430628.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            20606..20842
                     /locus_tag="N7C74_000692"
     CDS             20606..20842
                     /locus_tag="N7C74_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HGU2430629.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(20883..21539)
                     /gene="pphB"
                     /locus_tag="N7C74_000693"
     CDS             complement(20883..21539)
                     /gene="pphB"
                     /locus_tag="N7C74_000693"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HGU2430630.1"
                     /translation="MPSTRYQKINTHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(21645..24206)
                     /gene="mutS"
                     /locus_tag="N7C74_000694"
     CDS             complement(21645..24206)
                     /gene="mutS"
                     /locus_tag="N7C74_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HGU2430631.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            24282..24416
                     /locus_tag="N7C74_000695"
     CDS             24282..24416
                     /locus_tag="N7C74_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2430632.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSVG
                     "
     gene            24493..24846
                     /gene="ygbA"
                     /locus_tag="N7C74_000696"
     CDS             24493..24846
                     /gene="ygbA"
                     /locus_tag="N7C74_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HGU2430633.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKCP
                     VPELPEKYRPKKPRE"
     gene            complement(24883..26961)
                     /gene="flhA"
                     /locus_tag="N7C74_000697"
     CDS             complement(24883..26961)
                     /gene="flhA"
                     /locus_tag="N7C74_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HGU2430634.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(27035..28045)
                     /gene="hypE"
                     /locus_tag="N7C74_000698"
     CDS             complement(27035..28045)
                     /gene="hypE"
                     /locus_tag="N7C74_000698"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HGU2430635.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPTNIHWG
                     AQTLTAGDILLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(28042..29163)
                     /gene="hypD"
                     /locus_tag="N7C74_000699"
     CDS             complement(28042..29163)
                     /gene="hypD"
                     /locus_tag="N7C74_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HGU2430636.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     QRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(29163..29435)
                     /gene="hypC"
                     /locus_tag="N7C74_000700"
     CDS             complement(29163..29435)
                     /gene="hypC"
                     /locus_tag="N7C74_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HGU2430637.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(29426..30298)
                     /gene="hypB"
                     /locus_tag="N7C74_000701"
     CDS             complement(29426..30298)
                     /gene="hypB"
                     /locus_tag="N7C74_000701"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HGU2430638.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAC
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(30302..30652)
                     /gene="hypA"
                     /locus_tag="N7C74_000702"
     CDS             complement(30302..30652)
                     /gene="hypA"
                     /locus_tag="N7C74_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HGU2430639.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            30864..31325
                     /gene="hycA"
                     /locus_tag="N7C74_000703"
     CDS             30864..31325
                     /gene="hycA"
                     /locus_tag="N7C74_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HGU2430640.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            31450..32061
                     /gene="hycB"
                     /locus_tag="N7C74_000704"
     CDS             31450..32061
                     /gene="hycB"
                     /locus_tag="N7C74_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HGU2430641.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            32058..33884
                     /gene="hycC"
                     /locus_tag="N7C74_000705"
     CDS             32058..33884
                     /gene="hycC"
                     /locus_tag="N7C74_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HGU2430642.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLSVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            33887..34810
                     /gene="hycD"
                     /locus_tag="N7C74_000706"
     CDS             33887..34810
                     /gene="hycD"
                     /locus_tag="N7C74_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HGU2430643.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVASSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            34828..36537
                     /gene="hycE"
                     /locus_tag="N7C74_000707"
     CDS             34828..36537
                     /gene="hycE"
                     /locus_tag="N7C74_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HGU2430644.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            36547..37089
                     /gene="hycF"
                     /locus_tag="N7C74_000708"
     CDS             36547..37089
                     /gene="hycF"
                     /locus_tag="N7C74_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HGU2430645.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            37089..37856
                     /gene="hycG"
                     /locus_tag="N7C74_000709"
     CDS             37089..37856
                     /gene="hycG"
                     /locus_tag="N7C74_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HGU2430646.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            37853..38263
                     /gene="hycH"
                     /locus_tag="N7C74_000710"
     CDS             37853..38263
                     /gene="hycH"
                     /locus_tag="N7C74_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HGU2430647.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            38256..38726
                     /gene="hycI"
                     /locus_tag="N7C74_000711"
     CDS             38256..38726
                     /gene="hycI"
                     /locus_tag="N7C74_000711"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HGU2430648.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(38850..40274)
                     /gene="ascB"
                     /locus_tag="N7C74_000712"
     CDS             complement(38850..40274)
                     /gene="ascB"
                     /locus_tag="N7C74_000712"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HGU2430649.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATGFYHRYKEDIALMAEMGFKVFRTSIAWSRPFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMQLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNTNNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(40283..41740)
                     /gene="ascF"
                     /locus_tag="N7C74_000713"
     CDS             complement(40283..41740)
                     /gene="ascF"
                     /locus_tag="N7C74_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HGU2430650.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPALPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTLTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNSELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSAQPTVAKEVSLN"
BASE COUNT         9612 a        10853 c        11498 g         9883 t
ORIGIN      
        1 tgactttctt tgatttctgg ggaaaaaccc gacgtggcga gaaagagggt ggcgacggct
       61 atcaccttct ttgctggcat tcgctggatg tggccgcaat gggctattta atggttaaaa
      121 gaaattgctt cgggctggct gattactttc gtcaattagg gatttctgac aaggaacagg
      181 cggctcaatt tttcgcttgg ttgctgtgct ggcacgatat tggaaaattt gcccgctctt
      241 ttcagcaact ttacctggcc cctgaactca agattccgga aggttccaga aagaattacg
      301 aaaagatctc tcattcaacg ctgggttact ggctgtggaa ttattattta agtgaatgtg
      361 aggagttgct tccttcatct tcactctctt ctcgtaaact tacacgtgta atagagatgt
      421 ggatgtccat aactaccggg catcatggtc gaccacctga ccgtattgat gagctggata
      481 attttctgcc tgaagacaaa gctgccgcgc gagattttct ccttgaaatc aaggcactgt
      541 ttccgctcat agagattccc acattctggg atgatgacga gggcgttgaa cttttaaaac
      601 aactttcctg gtatatctct gcaacagtcg tactcgcaga ctggacgggt tcgtcaacgc
      661 gattttttcc acgcgtcgca cacccaatgg atattaaaga ttactggcag aaaactttag
      721 ttcaggctca aaacgcctta accgtctttc ctccaaaagc agaaaccgca cctttcaccg
      781 gaattaatac gctgtttcct tttattgagc acccgacacc attacagcaa aaggtactgg
      841 atctggatat cagccagcca gggccacagt tatttattct ggaagacgtg actggcgcag
      901 gtaaaacaga agcggcgctt atcctggcgc acaggttgat ggctgcgagg aaagcacagg
      961 gtttgttttt tggcctgcca acaatggcaa cggccaatgc catgtacgat cggctggtca
     1021 aaacctggct tgctttctat tcgccagagt cccgccccag cttggtgctg gcacacagtg
     1081 cccgcacatt aatggaccgc ttcaatgaat cactctggtc cggtgattta gtcgggtcag
     1141 aagaaccgga tgaacaaaca ttcagtcagg gatgtgcggc ctggtttgcc aacagtaaca
     1201 agaaggcgct actggctgaa attggcgtcg gcacgctgga tcaggcgatg atggcagtga
     1261 tgccgtttaa acataataat ctgcggcttc tggggttgag taacaaaatc ctgctggctg
     1321 atgagatcca tgcctgtgat gcttacatgt cgtgcattct tgaagggctg atcgagcggc
     1381 aggcgcgtgg cggaaacagc gtcattttgc tttctgctac gttatcccaa cagcagcgcg
     1441 acaaactcgt cgccgccttt gcgcgtggca cagagggcca gcaagaagct ccgttccttg
     1501 aaaaggatga ttacccctgg ctgacgcatg tcacgaaatc cgatgtgaac tcacaccggg
     1561 tagcgacgcg caaagacgtt gagcgtagcg tcagcgtggg ttggcttcat agtgaacaag
     1621 agagtattgc gcgtatcgaa tcggcggtaa gtcagggaaa atgcatcgcc tggatccgga
     1681 attctgtcga tgacgctatt aaggttcatc gtcagctgct tgcccgcggc gtcattcccg
     1741 cttccagcct ttcactcttt catagccgct ttgcttttag cgatcgccag cgaattgaaa
     1801 tggagacgct ggcacgcttt ggtaaagaag acggttcaca gcgtgccgga aaagtcctca
     1861 tttgtactca ggtcttagag cagagcgttg attgtgacct ggacgaaatg atctccgacc
     1921 tggcccctgt tgatttgctg attcagcgag cggggcgatt acagcggcat atccgcgata
     1981 ttaatggtca gttaaagcgt gacggaaaag acgagcgttc ccctcctgaa ttgctgattc
     2041 tggcccccgt ctgggacgac gctcctggtg acgaatggtt cggcagtgcc atgcgtaaca
     2101 gtgcatatgt ctatcccgat catggacgaa tctggctgac gcagcgtgta ctgcgtgagc
     2161 aaggcgctat tcaaatgcca cacgcagccc gccttcttat tgaatcagtc tacggtgagg
     2221 acgtggtaat gccggaagga tttgcccgca gcgagcagga gcaagtgggc aaatattact
     2281 gcgatcgcgc aatggctaaa aagtttgtcc tgaacttcaa gcctggctat gccgccaata
     2341 tcaacgatta ccttccggaa aagctgtcga cacgtctggc tgaggaatct gtttccctgt
     2401 ggctggctac ctgtattgcc ggtgtggtga agccttatgc caccggtgct cacgcatggg
     2461 aaatgagcgt tgtcagagtg cgtcgaagct ggtggaaaaa acatcgggat gagttttctt
     2521 tactggaagg ggaagcgttc aggcagtggt gcattgaaca gcggcaagat ccggaaatgg
     2581 caaacgtgat tttagtcact gatgacgaaa gttgcgggta ttcggccagg gagggattga
     2641 ttggcaaggt tgattgagat acctgcgaca ggactctctg aatagttgat acaccgaaaa
     2701 ggatggaatg atggacaacc gaccagggat ggtctttttt acgagaggat acatatgaac
     2761 tcgttttcac ttctgacaac cccgtggttg cccgttcgtt ttaaagacgg aacaacaggc
     2821 aagctggcgc cagtcgatct ggcggatgaa aatgttgtcg atatcgctgc gccgcgggca
     2881 gatctccagg gggcggcatg gcagtttttg ctggggttac tacaaagcag tttcgcgcca
     2941 aaagattatc gtcgttggga tgatatctgg gaagacgggc tggaagctga aaagctacgg
     3001 gaagcattgc tgtcattaga acaccctttc cagtttggcc cagattcacc ttcatttatg
     3061 caggatttcg aggtgctcat gggcgataaa gttcaggtcg cttcgctact gcctgagatt
     3121 cccggcgctc aaacaacgaa gtttaataaa gaccacttta ttaagcgtgg cgtgactgaa
     3181 cacgtatgct ctcattgttc tgcgttagct ctgttctccc tacagttaaa tgcgccgtca
     3241 ggtggcaaag gctatcgcac cggtttacgc ggcggtgggc cgatgacgac tctgattgaa
     3301 ttgcaggagt atcagggcaa tcaacaagcc cccttgtggc gcaaactgtg gctcaacgtg
     3361 atgccgcagg atgaagccga cttaccgcta cccaaaaaat ttgacgatct ggttttcccc
     3421 tggcttggcc cgacgcgtac cagcgaactg gccggtgcgg tggtaaccga tgatcaggtc
     3481 aataaactcc aggcgtactg gggaatgccg cggcgtattc gtattgattt taataccacg
     3541 acagtcggca actgcgatat ttgcggtgag cagagtgacg cgcttctgag tttgatgact
     3601 accaaaaatt acggtgcgaa ttatgccatg tggcagcatc ccttaacgcc ttaccgtgta
     3661 ccacttaaag agggcggtga gttttactcc gttaaaccac aaccgggcgg tttaatctgg
     3721 cgcgactggt taggccttat cgaaacgggt aagtcagaaa acaatacgga acttcccgcg
     3781 ctggtggtga aactctttaa tgccagcagt ctgaaacagg caaaagtggg cctgtgggga
     3841 tttggttatg atttcgacaa catgaaagcg cgctgttggt acgaacacca tttcccgctg
     3901 ctgctcaata aaaaagaagg ccagataccg aagctgcggc tggctgcgca aacggcttca
     3961 cggattctga gtctgttacg gagtgcattg aaagaagcat ggttctccga tccaaaaggt
     4021 gcaaggggtg atttcagttt tgtggatatc gacttctgga acaaaactca gcatcgcttc
     4081 ctgaggttag tgcgccaaat tgaagaaggt caggatgcgg atgaattact cggcaaatgg
     4141 caaaaggaaa tttggttatt cgcacgtcag gattttgacg agcgtgtatt caccaatcct
     4201 tatgagcccg ttgatttgga acgcgtcatg accgcgcgca agaaatattt tacaacatcg
     4261 gcggagaagc aaagtgctaa agccgccagg gagaaaaagc aggaggctgc tgaatgagta
     4321 ttgtaaaaga ggagcacaaa gccacgctgc gcaaatggca tgaggaattg caggagaaac
     4381 gcggcgaacg tgccagtctg cggcgtagta cgacggttaa tgatgtttgt ctgacggatg
     4441 gatttcgcct gtttctgaag aacagacaaa taaagtggca ggatgaacct gaatggcgta
     4501 ttaccgcact ggcgctgata gccgcactta gcgctaatgt taaagccatt gatgaacggc
     4561 tgccttttgc tgcgcagctg gcggcggtaa tgtcaaaggg gcgttttact cgcctgtcgg
     4621 cagtaaaaac accggatgaa ttactgcgcc agttacgccg cgtagtcaga ttattaaacg
     4681 gttccgttaa tcttgactct ctggcagagg gtgtcttccg ctggtgccag gagagcgacg
     4741 atctgctcaa tcatcaccgc cgtcatcagc ggccaaccga atttatccgt atccgctggg
     4801 cgctggaata ttaccaggct ggcgacgtcg ataacgaaca aaatcaataa aggactatct
     4861 catgacgaca tttattcagc ttcatttgtt aaccgcttac cctgcagcca accttaaccg
     4921 tgatgacacc ggagcgccga aaaccgtggt cctgggtgga gcaacgcgac tgcgcgtttc
     4981 ctcgcaaagt ctgaaacgtg cgtggcgcac ttctgcactt tttgaacagg cactggcggg
     5041 ccatattggt attcgcagtg ggcgtattgc gcgtgaggcg gcaactatcc tgattgagaa
     5101 aggcatcgaa gagaaaaaag ccatcgaatg ggcggcaaaa attgcggatt atcttgggaa
     5161 agctaaaaac gacaaaaaac caaaagatcc gctcactaac gccgaaactg aacaattagt
     5221 ccatatcagc ccggcagaat ttgacgccgt aaaagcgctg gcccatcaac tggccgaaga
     5281 aaagcgcgcg ccaaaagagg aagatctcgc tttgttacgt aaagatcgca tggcagtaga
     5341 tatcgctatg tttggtcgta tgctggcgaa taaacccgaa tttaatgttg aagccgcctg
     5401 ccaggtcgcg catgcatttg gtgtcagtga aacgattgtc gaagatgatt ttttcaccgc
     5461 cgttgatgat ttgcgccagg cttctgaaga tgccggtgcc gggcatcttg gtgaaaccgg
     5521 attcggttct gcgctgttct acacctatat ctgcatcgat aaagatctgc tggtcgaaaa
     5581 cctcggcggt gacgaagcgt tagctaatca gaccttgcgc gcctttacgg aagccgcact
     5641 taaagtctcc ccaaccggca aacagaacag ctttgccagc cgtgcctacg cctcctgggc
     5701 gctggcagaa aaaggcaccg aacaaccacg ttctctggcg gcggctttct atgaacccat
     5761 taatggcacc cggcagttag atgtggcggt gcagcgtatt acaacgcttc gcgaaaatat
     5821 gaatacggtc tatgaacaga agaccgaatg cgcaagcttt gacgtgatga acaaacaggg
     5881 aagcatgaag gacgtgctgg actttatctg cgcgtaaggg gcggctaatg agccaatatt
     5941 tgatttttca gcttcatggg ccaatggcat cctggggcgt cgatgccccc ggcgaagtgc
     6001 gtcataccca tgaactgcct tcgcgctcag cattgctggg gctgctggct gccggggtag
     6061 ggattcggcg tgatgatacc gaacgattaa acgcgtttaa ccgccactat tcactggtgg
     6121 tttgcgccag ccgtaacccg cgctgggcac gggattatca cacggtccag atgccaaaag
     6181 aggtgcgtaa agcgcgttat ttcagccgtc gcgaagagtt gagcgcccct gatcttctga
     6241 gcgcgattat ctcccggcgc gactactaca ccgatgcctg gtggatggtg gcggtggcaa
     6301 caacccccga tgcgccttac agccttgaac agttgcagga cggtttacgt catccggttt
     6361 ttccgcttta tctggggcga aaaagtcatc cgctggcgtt accacttgcg ccgttactgc
     6421 tcgaaggcaa cgcgtctgat gtcttacgta acgcatacca acagtatcag gatagtttcc
     6481 gcgaactgaa agtctcactc ccgaaacttc aggatgaatg ctggtgggaa ggggaacacg
     6541 acggcctggt cgcgagcaaa atattacgtc gccgggatgt tcctttaaat cgtcagcagt
     6601 ggctgtttgg ggaacgcacc atcaatcagg ggccgtggct cagcaaggag gaaccatgta
     6661 cctctcaaga ataaccttac ataccggcca gctttcgcct gcgcagttgc tgcatttggt
     6721 ggatcgcggg gaatatgtga tgcatcagtg gctgtgggat ctcttccctg gcggcaaaga
     6781 aaggcaattt ctttatcgtc gggaagaact ccagggcgcg tttcgctttt ttgtactgtc
     6841 gcaggaacgc ccggcggaaa gtgaaacgtt caccatcgaa tgccggtcat ttgtgcccga
     6901 gctgcgtacc ggacaacaac tttgtttcaa cctgcgggcg aatccaacaa tctgtaaagc
     6961 gggcaagcgc cacgatctgc tgatggaggc gaaacggcag gtgagggggc aggcggaagg
     7021 aagtgatgtc tggttgcatc aacaacaggc ggcgctggac tggctggcag cgcaggggga
     7081 gagaagcggt tttacactgc tggatacctc ggttgatgcc taccgacaac agcaactgcg
     7141 gcgggaaaac tcccggcaac tgatccagtt cagtagcgtg gattatacgg ggatgcttac
     7201 ggtcaccgat cccgggttat ttctgcaacg tctcagtcag gggtatggca aaagccgcgc
     7261 gttcggctgt ggtctgatgc tgatcaaacc cggagcagag gtgtgacgtt tgtaccactg
     7321 agtccgatcc cgttaaaaga tcgcacctct atgatcttcc tccagtacgg tcaaatcgac
     7381 gtactggacg gcgctttcgt gctgatcgac aaaaccggga tccgcacgca cattccggtg
     7441 ggatcggtcg cctgcattat gctcgaaccg ggaacgagag tttcccacgc ggcggtgcat
     7501 ctggccgcca cggtgggaac actgctggtc tgggtcggtg aagcgggcgt tcgcgtttac
     7561 tcttccggac aacccggagg ggcgcgggca gataaattac tctaccaggc aaagctggct
     7621 ttaacggaag atctacgcct gaaggtggtg cgcaaaatgt atgaattacg ttttcgtgag
     7681 ccaccgccag ctcgccgttc agtggatcag ctacggggaa ttgagggatc ccgcgttcgc
     7741 cagacctatg cattactggc gaaacaatat ggtgtgaaat ggaatggtcg caaatacgat
     7801 cctaaagact gggaaaaagg cgatgttgtg aatcgctgca tcagtgctgc cacatcatgt
     7861 ctgtacggta tttctgaagc ggcagtatta gccgcgggat atgcgcccgc tattggattt
     7921 attcatagtg gcaaaccgct ttcatttgtt tatgacatag ccgatatcat taaatttgat
     7981 tcggttgtgc caaaggcatt tgaaatagca gcgaggcaac ccgcagaacc tgataaagaa
     8041 gtcagattag cctgtcgcga tattttccgt agcactaagt taacgggcaa attaataccg
     8101 ttaattgaga aagtccttgc tgcaggtgaa attgaaccac cacaacccgc gccggatatg
     8161 ttaccgcctg ccatccctga acctgaaacg ctgggtgata gtggtcaccg ggggcgcggc
     8221 ggatgagcat ggtcgtggtt gttacagaaa atgtccctcc gcgcttacgt ggacggctcg
     8281 caatctggct actggaagtg cgtgccggtg tgtatgttgg tgatacatca aaacgtattc
     8341 gggagatgat ctggcagcaa attacccaac tggctggttg cggaaatgtg gtgatggcct
     8401 gggcgaccaa taccgagtcg ggttttgaat ttcagacctg gggagaaaac agacgtattc
     8461 cggtggattt ggatgggtta cgtttggttt cttttcttcc tgttgataat caataggtta
     8521 tgtgttcttt aaaaataagg aaatgtttga atttagttgg tagattgttg atgtggaata
     8581 aatttgttta aaaacagata tgtatgctta gtgtgttccc cgcgccagcg gggataaacc
     8641 gtcaccaaaa cagtgacaaa aactgtcacc aaagtgttcc ccgcgccagc ggggataaac
     8701 cgctcatatt cggattgatc gtgtgtttcg gtttgtgttc cccgcgccag cggggataaa
     8761 ccggcccagg gatttgttca atccagcgtg ccgctgtgtt ccccgcatca gcggggataa
     8821 accgggcgca ctggatgcga tgatggatat cacttagaat tccccgcccc tgcggtagaa
     8881 cacccagctc ccattttcca acccatcaag acgccttcgc caactccttc accagcggta
     8941 gcattatccg cataacgtca cggcagcgac gttctattct tccaggaagt gctttatcaa
     9001 tatgttgctg attatccagt cttacgtcat gccagctatt tcccgcaggg aatgcggctg
     9061 tttttgcgcg ttgctgataa ccatctttat tcccaagatt ccagtttgtc gcttccaccg
     9121 aaagtacagc aatgcccgct ttgtcgaata tttctgcgtc attacaacat ccagtgcctt
     9181 tcggataatt tttattcaaa cccggattgg tcgttgcggc aattccatga ctacgcgcaa
     9241 ttgccagcgc cctgtcgcgc gttaatttcc ttactgcttc aggggttttt acaccgctgt
     9301 tgaaatacaa tttatcgcca acaattaagt tatcgagatt aatcaccagc agcgtatttt
     9361 tcttttcggt gtcactcatc cgcttgagta aattctcagc gcctaatttc ccttcctctt
     9421 ctccactggt cgccacaaat cgaataccat actcggtagg cgtatttttc aggcgttctg
     9481 ccagttccag catgacacct aaacctgcgg cgttatcatc cattccttgt aacgtcagcc
     9541 cgccgagatt ggcatctgca tctgcgtcgc tctgcggggc ataggtatcc agatgcgcca
     9601 taataatgat ctgctgcggc gctttgcctt catgagcggc aatcaccgta cttcccgtca
     9661 cgttgtgcca gtttttgcgg ttatcgcggg cggtataaat atatcggcta ttaaacgtac
     9721 gaatatcact gcggtaaccc atttgctgaa actgttgccg aatataatcg gcagataaca
     9781 tttctgcggg tgttccggtc attcgtcccg gaaagaaagt ggcaatatgt cgcgcctgtg
     9841 tattggcaaa atcgccaggt ttaggtgacg aggcgtgtac ggggagaata aagcatacgc
     9901 cgagcgccag ggcagcggta cggtggcgca atgcggaaaa catagtgagt ccttaaatac
     9961 catgcaaatt tttttaccgc catagtatga aactgccgct gcgctaaaac aatttcaaat
    10021 cttcctaaac gcccgaaatc cggtgcctta agcacttttt gatattagct ttgccaaatc
    10081 gttattccgt taaggaacta ctcattctaa ttggtaattt cattcgttct cttacgctcc
    10141 ctatagtcga aacatctgat ggcaagaaaa tagcggtatt gcaaaggaac ggttatggat
    10201 caaatacgac ttactcacct gcggcaactg gaggcggaaa gcatccacat tattcgcgag
    10261 gtggcggcag aattctcaaa tccggtgatg ctctactcta tcggtaaaga ttccagcgtc
    10321 atgctgcatc tggcgcgcaa ggcgttttat ccaggtacgc tgcctttccc gttgctgcat
    10381 gtcgataccg gctggaaatt ccgtgagatg tatgagttcc gcgatcgtac ggcgaaagcc
    10441 tacggctgcg agctgctggt gcatcaaaac ccggaaggcg tggcgatggg gattaatcca
    10501 ttcgtgcacg gcagcgcgaa acataccgat attatgaaga ccgaaggcct gaagcaggcg
    10561 ctgaataaat acggttttga tgccgccttc ggtggtgcgc gccgcgacga agagaaatcc
    10621 cgtgctaaag agcgtatcta ctctttccgt gatcgcttcc atcgctggga cccgaaaaat
    10681 cagcgcccgg agctgtggca caactataac gggcagatta acaaaggcga aagcatccgc
    10741 gttttcccgc tctctaactg gaccgagcag gatatctggc aatacatctg gctggaaaat
    10801 atcgacattg tgccgctgta tctcgctgcg gaacgtccgg ttctggaacg cgacggtatg
    10861 ttgatgatga ttgatgacaa ccgtatcgac ctgcaaccgg gcgaagtgat taaaaaacgg
    10921 atggtgcgtt tccgtacgct gggctgctgg ccgctgaccg gtgcggtgga gtcaaatgca
    10981 caaacgctgc cggagatcat cgaagagatg ctggtttcca ccaccagtga acgtcagggg
    11041 cgcgtgattg accgcgacca agctggttct atggagctga aaaaacgtca ggggtatttt
    11101 taagatgaac accgcacttg cacaacaaat cgccaatgaa ggcggcgtcg aagcctggat
    11161 gattgcgcaa caacataaaa gcctgctgcg ttttctgacc tgtggtagcg tcgatgacgg
    11221 caaaagtacc ctgattggtc gtctgttgca cgatacccgc caaatctatg aagatcagct
    11281 ctcatcgctg cataatgaca gtaagcgtca cggcacccag ggcgaaaagc tggatctggc
    11341 tctgctggtg gacggcctgc aagctgagcg tgaacagggc atcaccattg acgtggccta
    11401 ccgctatttc tctaccgaga agcgtaaatt tattatcgcc gacactccag ggcacgagca
    11461 gtacacccgc aatatggcga ctggcgcatc gacatgtgaa ctggcgatct tactgatcga
    11521 tgcccgtaaa ggcgtgctcg atcaaacccg tcgtcacagt tttatctcca cactgttggg
    11581 gatcaaacat ctggtcgtgg cgatcaacaa aatggatctg gtggattaca gtgaagagac
    11641 gttcacccgt attcgtgaag attatctgac ctttgccggg cagctgccgg gtaatctgga
    11701 tatccgcttt gtgccgctct ccgcactgga aggtgacaac gtggcttcgc aaagtgaaag
    11761 tatgccgtgg tacagcggtc cgacactgct cgaagtgctg gaaaccgtgg agattcagcg
    11821 agtggtggat gcccagccaa tgcgcttccc ggtgcagtac gttaaccgcc caaatctcga
    11881 ttttcgtggc tacgccggaa cgctggcatc cggtcgcgtg gaagtcgggc aacgtgtaaa
    11941 agtgctgccc tctggtgtgg aatcaaacgt cgcgcggatc gtgacttttg atggtgatcg
    12001 cgaagaagcc tttgccggag aagctatcac cctggtgctg acggatgaga tcgacatcag
    12061 ccgtggcgat ctgctgctgg cggcagacga agcgttacca gctgtgcaga gcgcgtcggt
    12121 ggatgtggta tggatggcgg aacagccgct ttccccgggc cagagttacg acatcaaaat
    12181 tgccggtaag aagacgcgtt ctcgtgttga tggcattcgt tatcaggttg atattaataa
    12241 ccttacccaa cgcgaagttg aaaacctgcc gctgaacggc atcggcctgg tggatctcac
    12301 ttttgacgag ccactggtgt tagatcgtta tcagcaaaac ccggttaccg gtgggctgat
    12361 ttttatcgat cgcctgagca atgtgaccgt aggtgccggt atggtgcatg agccagttag
    12421 ccaggcaact gctgcgccat ctgaatttag tgcattcgaa ctggaattga atgccctggt
    12481 tcgccgccac tttccacact ggggcgcgcg cgatttgctg ggggagaaat aatggcgctg
    12541 catgacgaaa acgtcgtctg gcatagccat ccggtcactg tgcaacaacg cgagctacac
    12601 cacggtcatc gtggtgtagt gctgtggttt accggcctct ccgggtccgg taaatcaacg
    12661 gtcgccgggg cgctggagga ggcgttacat aaactcggcg tcagtacgta tctgctggat
    12721 ggcgacaatg ttcgccacgg attatgcagc gatctcggtt ttagcgatgc cgatcgtaaa
    12781 gagaatatcc gtcgcgtcgg tgaagtggcg aatttgatgg ttgaagccgg actggtggtg
    12841 ctgaccgcat ttatctcgcc acaccgcgcc gaacgccaga tggttcgcga acgcgtagga
    12901 gaagggcgct ttatcgaagt gtttgtcgat acgccactgg cgatttgcga agcccgcgat
    12961 ccaaaaggct tatataagaa agcgcgtgcc ggtgaactgc gcaactttac gggaatagat
    13021 tccgtttacg aagcgcctga atcggcagaa attcatctca atggtgaaca attagtaaca
    13081 aatttggtac agcaattatt agatctgttg agacagaacg atattatcag atcctgagac
    13141 accaccgggt ttccatgccc ggttcgtcaa agtaacagga tcaaatatgc gtaatagcca
    13201 taacattaca ctaacaaata acgacagcct tactgaggat gaagaaacca catggtcact
    13261 gcctggtgcc gtggtcggtt ttatctcctg gttatttgcg ctggcgatgc cgatgttgat
    13321 ttatggctct aacacgctgt tcttctttat ctacacctgg cctttctttc tggcgctgat
    13381 gcccgttgcg gtagtggtgg ggattgcgct gcattcattg atggacggaa agctacgcta
    13441 cagtattgtt ttcaccctga tgacagtagg cattatgttt ggcgcactgt ttatgtggct
    13501 actgggctaa tttgtacttt cttgtctcct ctgttcataa ttcaaaccgt aactaataat
    13561 gagattatgt tctgcacgcc ctgggtatac gtaacaatgg acaaatgtgg tacatttgcc
    13621 cgcgttgtcg cggtatcccc aacagaggat gtagagtcgt cttcggatgc atgggatgat
    13681 gatgccgttt ttcagggggc aggatgggta aactaacgct gctgttgctg gctattctgg
    13741 tctggctaca gtattcgctg tggttcggta agaacggtat acatgactat acccgcgtca
    13801 atgatgatgt ggcggcacag caagctacaa acgcgaaact taaagcgcga aacgatcaac
    13861 tttttgccga aattgacgat ctcaatggcg gccaggaggc gctcgaagag cgtgcgcgta
    13921 atgaactcag catgaccagg ccgggcgaaa ctttttatcg tctggtgcct gacgcgtcga
    13981 agcgcgcaca gtctgcgggg caaaacaatc gataaatcag cccgggaatt aacatggcaa
    14041 ccactcattt ggatgtttgc gccgtggttc cggcggccgg atttggccgt cgaatgcaaa
    14101 cggaatgtcc taagcaatat ctctcaatcg gtaatcaaac cattcttgaa cactcggtgt
    14161 atgcgctgct ggcgcatccc cgggtgaaac gtgtcgtcat tgccataagt cctggcgata
    14221 gccgttttgc acaacttcct ctggcgaatc atccgcaaat caccgttgta gatggcggtg
    14281 atgagcgtgc cgattccgtg ctggcaggtt tgaaagccgc tggcgacgcg cagtgggtat
    14341 tggtgcatga cgccgctcgt ccttgtctgc atcaggatga cctcgcgcga ttgttggcgt
    14401 tgagcgaaac cagccgcacg ggagggatcc tagccgcacc agtgcgcgat actatgaaac
    14461 gtgccgaacc gggcaaaaat gccattgctc ataccgttga tcgcaacggc ttatggcacg
    14521 cgctgacgcc gcaatttttc cctcgtgagc tgttacatga ctgtctgacg cgcgctctaa
    14581 atgaaggcgc ggctattacc gacgaagcct cggcgctgga atattgcgga ttccatcctc
    14641 agttggtcga aggccgtgcg gataacatta aagtcacgcg cccggaggat ttggcactgg
    14701 ccgagtttta cctcacccga accatccatc aggagaatac ataatgcgaa ttggacacgg
    14761 ttttgacgta cacgcctttg gcggtgaagg cccaattatc attggtggcg tacgcattcc
    14821 ttacgaaaaa ggattgctgg cgcattctga tggcgacgtg gcgctccatg cgttgaccga
    14881 tgcattgctt ggcgcggcgg cgctggggga tatcggcaaa ctgttcccgg ataccgatcc
    14941 ggcatttaaa ggtgccgata gccgcgagct gctacgcgaa gcctggcgtc gtattcaggc
    15001 gaagggttat acccttggca acgtcgatgt cactatcatc gctcaggcac cgaagatgtt
    15061 gccgcacatt ccacaaatgc gcgtgtttat tgccgaagat ctcggctgcc atatggatga
    15121 tgttaacgtg aaagccacta ctacggaaaa acttggattt accggacgtg gggaagggat
    15181 tgcctgtgaa gcggtggcgc tactcattaa ggcaacaaaa tgattgagtt tgataatctc
    15241 acttacctcc acggtaaacc gcaaggcacc gggctgctga aagccaatcc ggaagacttt
    15301 gtggtggtgg aagatttggg ctttgagcct gatggtgaag gtgagcatat tctggttaga
    15361 atcctcaaaa acggctgcaa tacccgtttt gtggcggatg cactggcgaa attcctgaaa
    15421 attcatgccc gtgaagtcag ctttgctggg caaaaagaca aacatgctgt tacagaacag
    15481 tggttatgcg ctcgcgtgcc gggcaaggaa atgcccgatc tgagcgcctt tcaattggaa
    15541 ggctgccagg tgctggagta tgcgcggcac aagcgcaagc tgcgtttagg ggcgctgaaa
    15601 ggtaacgcct ttaccctggt tctgcgcgaa gtgagcaatc gcgatgacgt tgaacaacgt
    15661 ctgatcgata tttgcgtaaa aggtgtaccg aactacttcg gtgcccaacg ttttgggatt
    15721 ggcggtagca acttgcaggg cgcgctgcgc tgggcgcaaa ccaatactcc ggtgcgcgat
    15781 cgcaataaac ggagtttttg gttgtcggca gcccgcagtg cgttgtttaa tcagattgtt
    15841 gctgagcgcc tcaaaaaagc agacgttaat caagttgttg acggcgatgc gctacaatta
    15901 gccggacgtg gtagctggtt tgttgcaacc accgaagaac tggtggaatt acagcgtcgc
    15961 gtcaacgata aagagctgat gataaccgcc gcattgccgg gcagtggtga atggggaact
    16021 cagcgtgaag cgctggcatt cgaacaggca gctgtcgccg cagaaactga attacaagct
    16081 ttactggtgc gcgaaaaagt tgaagccgcg cgcagagcga tgctgctgta tccgcaacaa
    16141 ttaagctgga attggtggga tgacgtcacc gtagaaatcc gtttctggct tccggcgggt
    16201 agttttgcaa ccagcgttgt cagggaactt atcaacacaa caggtgatta tgcgcatatt
    16261 gctgagtaat gatgacgggg tacatgcacc cggtatacaa acgctggcga aagccttgcg
    16321 tgagtttgct gacgttcagg tggtcgcccc cgatcgtaac cgcagcggcg cttcaaattc
    16381 tctgacactg gaatcctccc tgcgcacgtt tacctttgaa aatggtgata ttgctgtgca
    16441 aatgggaacc ccgaccgatt gcgtctatct tggcgtgaat gctctgatgc gtccgcgccc
    16501 ggacattgtt gtgtccggaa ttaacgccgg gccgaatctg ggggatgatg ttatttattc
    16561 cggtacggta gccgccgcga tggaaggccg tcatttaggt tttccggcgc ttgccgtctc
    16621 gcttgacggg cataaacatt acgacactgc cgcggcggta acctgttcaa ttttgcgcgc
    16681 actgtgtaaa gagccgctgc gcaccgggcg tattcttaat attaatgttc cggatttacc
    16741 cttggatcaa atcaaaggta ttcgcgtgac gcgctgcggt acacgacatc cggcagatca
    16801 ggtgatcccg cagcaagatc cgcgcggcaa tacgctgtac tggattggcc cgccgggcgg
    16861 taaatgtgat gccggtccgg ggaccgattt tgctgcggta gatgagggct atgtctccat
    16921 cacgccgctg catgtggatt taactgcgca tagcgcgcaa gatgtggttt cagactggtt
    16981 aaacagcgtg ggagttggca cgcaatggta agcagacgcg tacaagcact tctggatcaa
    17041 ttacgtgcgc aaggtattca ggatgagcag gtgctgaatg cacttgccgc cgtgccgcgt
    17101 gaaaaattcg ttgatgaagc gtttgaacaa aaagcctggg acaatatcgc cttgccgata
    17161 ggtcaggggc agacgatttc gcagccgtat atggtggcgc gaatgaccga attactcgag
    17221 ctgacgccgc agtcgcgggt gctggaaatt ggcaccggtt cgggatatca aacggcaatc
    17281 ctggcgcatc ttgtccagca tgtttgctcg gttgaacgga ttaaaggctt gcagtggcag
    17341 gcgcgtcgcc gcctgaaaaa tcttgattta cataatgttt caacccgtca tggcgatgga
    17401 tggcaaggtt ggcaggcacg tgcgccgttt gacgctatca ttgttacggc ggcaccgccg
    17461 gaaattccaa ctgcgctaat gacgcagctg gacgaaggcg ggattctcgt cttacccgta
    17521 ggggaggagc accagtattt gaaacgggtg cgtcgtcggg gaggcgaatt tattatcgat
    17581 accgtggagg ccgtgcgctt tgtcccttta gtgaagggtg agctggctta aaacgtgagg
    17641 aaatacctgg atttttcctg gttattttgc cgcaggtcag cgtatcgtga acatcttttc
    17701 cagtgttcag tatggtgcct tgcacggtaa ttatgtcact ggttattaac caatttttcc
    17761 tgggggataa atgagcgcgg gaagcccaaa attcaccgtt cgccgcattg cggctttgtc
    17821 actggtttcg ctatggctgg caggctgttc tgacacttca aatccaccgg caccggtcag
    17881 ctccgttaat ggcaatgcgc ctgcacatac caattctggt atgttgatta cgccgccgcc
    17941 gaaaatgggg acgacgtcta cagcgcagca accgcaaatc cagccagtgc agccagtagc
    18001 tcagcagccg gtacagatgg aaaacggacg catcgtctat aaccgtcagt atgggaacat
    18061 tccgaaaggc agttatagcg gcagtaccta taccgtgaaa aaaggcgaca cgcttttcta
    18121 tatcgcctgg attactggca acgatttccg tgaccttgct cagcgcaaca atattcaggc
    18181 accatacgcg ctgaacgtcg gtcagacctt acaggtgggt aatgcttccg gtacgccaat
    18241 cactggcgga aatgccatta cccaggccga cgcagcagag caaggagttg tgatcaagcc
    18301 tgcacaaaat tccaccgttg ctgttgcgtc gcaaccgaca attacgtatt ctgagtcttc
    18361 gggtgaacag agtgctaaca aaatgttgcc gaacaacaag ccaactgcga ccacggtcac
    18421 agcgcctgta acggtaccaa cagcaagcac aaccgagccg actgtcagca gtacatcaac
    18481 cagtacgcct atctccacct ggcgctggcc gactgagggc aaagtgatcg aaacctttgg
    18541 cgcttctgag gggggcaaca aggggattga tatcgcaggc agcaaaggac aggcaattat
    18601 cgcgaccgca gatggccgtg ttgtttatgc cggtaacgcg ctgcgggaga tgccgaatgt
    18661 cgagactcat ctggtgatgt cgaagtgggc gaaaaccacc attgaactgg aaacgcctta
    18721 cagcgcccgc gatgttgctg ccctcgctga cttcagccat aacccggcgg atcaggcggc
    18781 gaccatctcc tcaggttcct ttcgtacaga cggcatgatc gttattccgt gcagtatgaa
    18841 aacgctcgcc ggtatccgcg ctggttacgc tgatggcctg gtagggcgcg cggcggacgt
    18901 cgtgcttaaa gaaggccgca aactggtgct ggtgccgcgt gaaatgccgc ttagcaccat
    18961 ccatctcgaa aatatgctcg cactttcacg catgggcgtg gcgatggtgc cgccgatgcc
    19021 tgccttttat aaccatcccg aaacggtaga tgacattgtc caccacgtgg tagcccgcgt
    19081 gctggatcaa tttggccttg aacatcccta cgccaggcgc tggcaaggat tgccgcaggc
    19141 ccggaatttt tctcaggaga atgaataatg gcatttgatg atttacgcag ctttttacag
    19201 gcgcttgatg accacggcca gttactgaaa atcagcgaag aggtgaacgc ggaaccggat
    19261 ctggctgccg ctgccaacgc caccgggcgt atcggcgatg gtgcaccggc gctgtggttt
    19321 gataatattc gcggttttac cgatgcccgc gtggcgatga ataccatcgg ttcctggcag
    19381 aaccacgcga tttccttggg cctgccgcca aacaccccgg ttaaaaaaca gattgatgag
    19441 tttatccgcc gctgggataa cttcccgatc gccccggagc gccgcgccaa tccagcctgg
    19501 gcgcagaaca ccgtcgatgg tgacgagatt aatctgttcg atatcctgcc gctgtttcgt
    19561 ttaaacgacg gtgatggcgg tttctatctc gacaaagcgt gcgtggtttc ccgcgatccg
    19621 ctcgacccgg ataacttcgg caagcagaac gtcggtatct accgcatgga agtgaagggc
    19681 aagcgtaagc tcggcctgca accggtgccg atgcacgata tcgccctgca tctgcataaa
    19741 gcggaagagc gcggtgaaga tctgccgatt gccatcaccc tgggtaacga tccgatcatc
    19801 acccttatgg gcgccacgcc gctgaaatac gatcagtctg agtatgaaat ggcaggcgcg
    19861 ctgcgcgaaa gcccgtaccc gatcgccacc gcgccattga ctggttttga tgtgccgtgg
    19921 ggttcagaag tgatcctcga aggggttatc gaaagccgca aacgcgaaat cgaagggccg
    19981 ttcggtgagt ttaccgggca ctactccggc gggcgtaaca tgaccgtggt gcgtatcgat
    20041 aaagtctctt accgcaccag gccgattttc gaatcgctgt acctcggcat gccgtggacc
    20101 gaaatcgact acctgatggg gccagccacc tgtgtgccgc tgtatcaaca actgaaagcc
    20161 gagttcccgg aagtgcaggc ggtaaacgcc atgtacaccc acggcctgct ggcgattatc
    20221 tccaccaaaa aacgctacgg cggctttgcc cgcgcggtgg gcctgcgtgc gatgaccacg
    20281 ccgcacggtc tgggctacgt aaagatggtg attatggtcg atgaagacgt tgacccgttc
    20341 aacctgccgc aggtgatgtg ggcgctctcc tcgaaagtaa atccggcagg ggatttggtg
    20401 cagttgccga atatgtccgt gctggaactc gaccctggct caagcccggc ggggatcacc
    20461 gacaagctga ttatcgacgc caccacgcct gtcgccccgg acaaccgtgg tcactacagc
    20521 cagccggtgg tggatttgcc ggaaaccaaa gcctgggctg aaaaactgac cgctatgctg
    20581 gccgcacgta aataaggaga acaagatgat ttgtccacgt tgtgccgatg aacagattga
    20641 agtgatggcg aaatcgccgg tgaaagatgt ctggacggta tatcagtgcc agcattgcct
    20701 ttatacctgg cgcgataccg aaccgctgcg tcgtaccagc cgcgaacatt atcccgaagc
    20761 gttccgcatg acgcagaaag atattgatga cgcgccaatg gtgccgagca tcccgccgct
    20821 gctggcggaa ggtaagcgct aataaaaagt gccgggtagt ggctttgctt tacccggcct
    20881 cattacttta ttttataaaa tgacagccgc ccgcttttcg gcgatccggt atcaatataa
    20941 atctggttag cgaacgtctg aatgttatca aacatcatat gtccaaatat aaaataatca
    21001 gcgccgttta tttgttgtaa ctcgccatta agcgatttct gcacacgatc aacaggccag
    21061 agtaattcgc tctccgctat ttctttacca aagagatatt cactccccgg ataatctgca
    21121 tgtgcgatga catattttat gttgtcgtta gtgatttcaa taatatgtgg aaggtgatgg
    21181 aatttcagca acagatctgt agcttctttt tgctctgaat catttaaatc gaaaaaccag
    21241 tcaccaccgc tggcaagcca catattgcca tcgccggtcg cgaacgcatc cagcgccatt
    21301 gcttcgtggt tgcctttaac ggagataaac cagggctggt ttagcaggcg caggacgtta
    21361 agactctccg gcccacgatc aatattatcg ccgacagaaa taagtaagtc ggtttcgggg
    21421 aaaaaagaga gttgatgtaa gcgggattgt aataactgat attcaccatg aatatcacca
    21481 acgacccata tatggcgata gtgatgggta ttgatttttt gatagcgtgt agatggcatg
    21541 gttttaccct gtaaaataag ctttcctatt atacagggta tttttatttg attcgtcagt
    21601 tatcgttaat attcccgata gcaaaagact atcgggaatt attattacac cagactcttc
    21661 aggcgataaa tccattccag cgcctgacgc ggggtgagtg aatccggatc gaggttttcc
    21721 agtgcctcga ctgcaggcga ggtttcttcc ggtacggaca gcaaagacat ttgcgtacca
    21781 tccacttgcg tagcggcggc gttcggcgaa atgctttcca gctcacgcag tttttgccgt
    21841 gcgcgcttaa taacctcttt tggcacgccg gccagagctg caaccgccag gccgtagctt
    21901 ttgctcgccg cgccatcctg cacgctatgc ataaaggcaa tggtgtcgcc gtgctccagt
    21961 gcatcgagat gcacgttggc gacgccttcc attttctccg gtaactgggt cagctcgaaa
    22021 tagtgggtgg caaacagcgt caacgcctta atcttattcg ccagattttc cgcgcacgcc
    22081 cacgccagcg acagaccatc gtaagtggac gttccgcgcc caatctcatc catcagcacc
    22141 agactgtact cggtggcgtt atgcagaata ttagcggttt cggtcatctc caccataaag
    22201 gttgaacgcc cggaagccag atcatccgct gcccctacgc gggtaaagat acggtcaatc
    22261 gggccaatct cgactttttg cgccggtacg tagctgccga tataggccat cagcgcaatc
    22321 agtgcggtct ggcgcatata ggtactttta ccgcccatgt tcggaccggt aataatcaac
    22381 atccggcgct gcggtgacag attcagcggg ttggcgataa atggctcgtt cagcacctgt
    22441 tcaaccaccg gatggcggcc ttcggtaatg cgaatgcccg gtttatcaat gaaggtcggg
    22501 caggtgtagt tcagggtata ggcccgttcc gccaggttca ccagcacgtc gagttccgcc
    22561 agcgcgctcg cgctctgttg caacgcttcc agatgcggca acagcaggtc gaacagctct
    22621 tcataaagct gtttttccag agccagtgct ttgccttttg aagtgaggac tttatcttcg
    22681 tactctttta gctctggaat gatgtagcgc tcggcgtttt tcagcgtctg gcgacgcata
    22741 tagttgatag gtgccagatg gctttgccca cggctgattt gaatgtagta gccgtgcacc
    22801 gcattaaagc caacttttag cgtgtccagg ccggtacgtt cacgctcgcg gacttccaga
    22861 cgctccagat aatcggtcgc gccgtcagcc agcgcgcgcc actcatccag ctcttcgtta
    22921 tagcctgatg cgataacacc accgtcgcgt accagcaccg gcggtgtgtc gatgattgct
    22981 cgctccagca gatcgcgcag ctcggcaaac tcgcccatct tctcacgtag cgcctgtact
    23041 ggtgcactat caacagtttc taactgcgca cgcagctccg gcagttgctg gaaagcgtga
    23101 cgcatacggg ccagatcgcg tgggcgagcg gtacgcaacg ccagacgcgc cagaatacgt
    23161 tccaggtcgc cgacctgacg tagtaccggc tgcaactcgg cggtgaaatc ctgcaatgcg
    23221 ccaatagttt gctggcgctc aagcaacacg cgggtatcgc gcactggcat atgcagccag
    23281 cgtttcagca tacggctgcc catcggcgtg acggtgcagt cgagcacaga agccagcgta
    23341 ttttccgcac cacccgccag gttctgggtg atttccagat tacgacgcgt cgcggcatcc
    23401 ataatgatgc tgtcctgctc acgttccatg gtgatggaac gaatatgcgg cagagtcgta
    23461 cgttgggtat ctttcgcata ctgcaacaga caaccggcag cacaaagtcc gcgcggcgcg
    23521 ttctcgacgc caaaaccgac cagatcgcgg gtcccaaatt gcagattcaa ctgctggcgc
    23581 gcggtgtcga tttcaaactc ccacagcggg cgacggcgca ggccgcgacg gccttcaatt
    23641 aacgacattt cagcaaaatc ttctgcatac agcagttccg caggattagt gcgttgcagt
    23701 tctgccgcca tcgtttcccg gtcagccggt tcgctcaggc gaaaacgacc ggaactgata
    23761 tccagcgtcg cgtagccgaa acctttgctg tcctgccaga tagccgccag caggttgtcc
    23821 tgacgctcct gcaacagggc ttcatcgctg atggtgcctg gcgtaacgat acgcacaact
    23881 ttgcgctcaa ccggaccttt gctggtcgcc ggatcgccaa tttgttcgca gatggcaacg
    23941 gactcgccct gattcaccag tttggcgagg tagttttcca ccgcatggta gggaatcccc
    24001 gccatcggga tcggctctcc cgccgaagca ccgcgtttgg tcagtgaaat atccagcagt
    24061 tgcgacgcgc gttttgcgtc gtcataaaac agttcataaa aatcacccat ccggtaaaac
    24121 agcaggatct cgggatgctg ggctttcagc ttgagatact gctgcatcat gggcgtatgg
    24181 gcgtcgaaat tttctattgc actcatgggg ttatgtccgg ttccctgata ttaaatgggg
    24241 tgtgatggtt tttattttcg ttgtaatcac ataaggcgca catgataccg gagttaatca
    24301 tttcagggga agaagcgaac ggcgagttct tgatgctggt tccgggaaag atgctgtttt
    24361 gcagcttttg cgtgatgctt atgaagcaag tacgcaaaat tgtatcggtt ggctaaccct
    24421 tcttctttag agtcatagtg ccctttaaga ttaagttgta atataaatgc atcttaatga
    24481 ggtggaggca gtatgtccgg caagcgtatc tctcgtgaaa aactgacgat taaaaaaatg
    24541 atcgatcttt atcaagcgaa atgcccgcag gcgtcagcgg agccggagca ttacgaggcg
    24601 ttgtttgttt acgcgcaaaa gcggctggat aaatgtgtgt tcggcgaaga gaaaccagcc
    24661 tgtaagcagt gtccggtgca ctgttaccag cctgcaaagc gtgaagagat gaaacagatt
    24721 atgcgttggg ccgggccgag aatgctgtgg cgtcatccga tcttaaccgt gcgtcatctc
    24781 atcgatgaca agtgtccggt gccagaactg ccggaaaaat atcgtccgaa aaaacctcgt
    24841 gaatagtatg ccggacaggc agatctgtcc ggcaatttgc agttaaatca atgccgattt
    24901 atcaattccc agtcgcttca tccgtgacag cagggtcgtg cgtttcagcc ccagacgttg
    24961 cgcagcgcct ttaggcccgg caaccacgcc gttagtttct ttcagcacgc gaacaatcaa
    25021 ctgatattca tcttcgccct cctgagcgac aaccgttgcg gcaggcggcg tttcaggctc
    25081 cggtaaagca atatctggca atgacagctg cagcacgttg ccgcgtgtta gcaataccgc
    25141 gcgctcaatg acgttttcca gttcgcgtac gttacccggc cactccatat tgctcaaggt
    25201 gcgcaacgtc tcggcaggaa tgctgtcgat attgcgcccc agacgacggg caattttgaa
    25261 ggtaaaggct ttcgccagca gcggaatatc ttccggacgc tcgcgtagtg gcggcaggtg
    25321 aatcgggaat acgttcaggc ggtaatagag atcgctacgg aactcacggt cggcgaccat
    25381 ttttttcaga tcgcggttag tcgcggcgat tagacgcacg tccgtctgaa tgattttgtt
    25441 gctgccgagg cgttcaaact cctgttcctg caatacacgc agcaacttcg gctgtaactc
    25501 cagtggcata tcgcccactt cgtcgaggaa cagggagctt ttatccgcca gttcaaaacg
    25561 accgatacgc tgggcgctgg caccggtaaa agccccacgc tcatgaccaa acagatcgct
    25621 ctccagcaat ccggcaggca tcgccgcgca gttcattttg accatgcggc gattattacg
    25681 cccactgaga ttatggatag cacgggcaat cagctcttta cccgtgccag tttcaccgag
    25741 gatcagcacg gtactgtcac tttgcgccac catttcaact tgtttaagca cgctgtacat
    25801 ggcttcgctg cggccaataa tctcgccaaa ttcactatca acattgttga gctgctcggt
    25861 cagggcgagg ttttcatcaa ccagccgttc tttcagacga tggatttcct gataggcgag
    25921 ggcgttatcg acagcgattg ccacacgttc ggcaatctgg cgcagtaaat tcagattggt
    25981 agtggtaaac actttctctt cacattgcgc cagtttcagc acgcccagca tggtgtcgcc
    26041 agacatcagc ggtaacaggc acaaggtttg aatctggttg ccccaggtat cgaacaacat
    26101 gcgttcatag ggggctaaat catcccgctc gtggagatta atcagcagca tctctttact
    26161 tttgaacacg cgttcggtga gggttccggc ttcatcgact tcgctctgtt cgtgggcggg
    26221 atgctgttta tcaagatagt gagtggagta gatgttgagt ttgtttttac ggtggctgcg
    26281 taagacgata ctgatatcgt caatatcgaa atagtaatgg atttctttgg cgacttcgct
    26341 gaccagttcg tccatatcca ggcgggaaag caccgcgttg gtgatggcga ccaggatgcg
    26401 gaagttatcg cgttcccggc ataacaactc atagtcgaca ttgttaacga cgcgactctg
    26461 gatttgttcg gtgacgacag aaacgatctg cgtaaatgtt tgcagacgat tgaactcttt
    26521 ttcgctccag gggcgatcgt cataacgaat aaattcacag ccaccaaaaa tatgcccttc
    26581 cgccgccagt ggcatcaggc aatagtgacc aaattttggg tatagcccac ctgcggccag
    26641 ctgcggccag gtttcacaaa attcttcgta actgcaatgc aacgtatcag ggcgcgacaa
    26701 aatgctgcgt accggaccgt gtgccagaac agtttcgtct tcatatttaa tgggggtgtc
    26761 tttttcacgc gatgcgtaat aagacgcacg ttgagtctgc gcttgccaca acacaatagc
    26821 cgcgttgtcg gcgagcgcag aacgctttac cagttgcgaa agagcctcac acagcgaggc
    26881 cagatcgggc tgctgcaata gtgtccgagt gatgtcgaac aacccttgct gtccgagatc
    26941 actcatcggt gtatatgaca ttttgttctt ccaggaaagc accgcaaagc ggtgcaaaag
    27001 gtcattgact aactatagga gatttagaat tttattagca tatacgcgga agcggttcgg
    27061 cgtgtggtaa atcgagggtt cgtttcacgc catacagacc ggcaagacga acacctttac
    27121 gttccaccac ttcaccaatc agcgccgcgt ctttccccag tggatgggaa tgtaacgctg
    27181 ccagcacttg ctctgccgcg ttgcgttcaa cggcgatcac cagtttgcct tcgttggcaa
    27241 agttcagggc gtccagtccc agcaattcgc aaacgccgcg cacggcaggt ttaaccggca
    27301 gcgctgattc agaaatttca ataccgcaac cgcaggctgc cgcgaactca tgaaccaccg
    27361 cgtttacacc accacgggtg gcatcacgca gcgctttcac gccgggaatg tcacgcagcg
    27421 tctgaataag cggcgtcagc accgcgcagt cgctgaccag ttcgccatcc agccccagct
    27481 gctcacgcag gttaaggata gtcgccccgt ggtcgccgag tgtaccgcta accagcaata
    27541 tatcgcctgc ggttagcgtc tgtgcgcccc agtgaatatt cgtcggaatt gcgcccatgc
    27601 ccgcggtgtt gataaacagt ttatctgccg cgccgcgctg caccacttta gtatcgccag
    27661 taacgatggc aatgcctgcc gtgcgggcgg tttctgccat gctggtcact acggctttca
    27721 gtgtctccat cggcaatcct tcttcgagga taaagccaca ggagagatag cgcggaatag
    27781 cgccactgac cgcaacgtca ttcgcggtgc cgcaaatcgc cagcttgccg atattaccgc
    27841 cagggaagaa cagcgggtca ataacgtaac tgtcggtgga gaacgccaga cggtcgcctt
    27901 ccgctaccag ctgcgccaga tcaagacgtg cctgatcttc ctgctctgcc agccacgggt
    27961 tggcaaaggc ttccataaac aggctgttga ttaattgctg catcgcctgg ccgccgctac
    28021 cgtgggcgag ttggatatta ttcacgcttc actctcctgc tgacgatact gataccacgc
    28081 ggcgcacgct ccttcggagg aaaccatcag cgcaccaaac gcggtttgag gattacaggt
    28141 gttaccaaac agcgggcatt gatgcggctt acatttgccc gtcaagactt cgccacaacg
    28201 cgcgcgcggg tcatcgcaga cctgctgcgg tgccgggcgg aaatgtgctt cggcatcgaa
    28261 tcgttgataa tccggcgtca ggtgcacgcc agaagattca atcacgccta agccgcgcca
    28321 ttcgctgtcg ccgttgacac agaacacatc ggcaatcgcc tgttgcgcca gcaggttacc
    28381 ggcatccggt accacccgac gatactgatt ctctaccttg ctgtgggccg ctattttctg
    28441 ctccaccagc atgaccacgc cttgcagtag atcaaggggt tcgaaaccag ccaccaccag
    28501 cggacgctga aaatcgctgg cgataaaatt ataggcatca gtgccgataa ccatactaac
    28561 gtggcccggc gcgaggaacg cgtcgatacc gttatccggc tgttccagca aactgcgcag
    28621 cgtcgggata agcgtaatat gctggcagaa gaagtaaaaa ttctgcacat cacgcgcttt
    28681 cgcctgttgc agagtgatgg cggtggtcgg catggtggtt tcaaaaccta agccgaagaa
    28741 caccactttg cgggttggat tctcctgcgc cagtttcaac gcatccatcg gcgaatagac
    28801 gatgcgcaca tcggcaccgc gtgcttttgc ctgcaacagc gatccctgtt tccccggcac
    28861 gcgcatggcg tcgccaaagg tacagaagat gacttccgga tggctggcaa tctccacgca
    28921 ggtgtcgatt ctgcccatcg gcagtacgca caccgggcaa cccggaccgt ggataaactc
    28981 aacgttttca ggcagtaact ggtcgaggcc gaatttaaaa atggcgtggg tatgaccacc
    29041 gcacacttcc ataatccgca gagggcgttc ggcggtgtaa gagagatgtg aagcacgttc
    29101 gcgcagatgc tcaattaact gcatcacctg ttccggcgcg cgatattcat caacaaaacg
    29161 cattattttt cctcgccata caacagcgcg ccgacatccg gctcaacgtc aaacatgttt
    29221 tgcaaggcgt cgagagtgtc gcgtgcttcg gcttcattaa ttacgctcat ggcaaagcca
    29281 acgtgtacca gtacccactg gcccacgcgc ggctgaccgt tttcatcgca gctgccgact
    29341 aacgttaaat cgacatcgcg ctgaatgccg cagacgtcga ctttcgcctg gttaccgtca
    29401 atggtgcgga tctggccggg aacgcctatg cacatcgctg tgtctccagc cagttcagcc
    29461 actggtccat cccttcgccg ctggtggcgg aaataaggat gatttcaatt tctggattga
    29521 cttcgcgggc gcaggcgatg cacttctcaa cgtcaaagtt gagatacggc aacaggtcaa
    29581 ctttgttgag cagcatcagc gaggcggcgg caaacatatg cggatatttc agcggtttgt
    29641 cttcaccttc ggtaacggaa agcaccgcca ctttgtgttt ttcaccgaga tcgaagctgg
    29701 ccgggcatac gaggttgcca acgttttcga taaacagaat accgttatcg tccagtggca
    29761 gacgcggtgc ggcgtcggca atcatctgtg cgtcaagatg gcagccttta ccggtgttca
    29821 cctgaatcgc tggtgtgccg gtagcgcgaa tgcgtgcggc atcgttcacg gtttgctggt
    29881 caccttcaat aaccgcgcac ggaacgctgt ctttcaggcg cattaaggtt tccgtcagca
    29941 gggtggtttt accggaacct gggctggaaa ccaggttgag caccagttgc ttgcaggcag
    30001 caaagcgcgc gcggttgcgt tcagccagac ggttattttt gtccagcacg tcaatttcga
    30061 cttccagcat ccgacgctgg ctcatgcccg gtgcgtgagt gcccgcttcg ccatgaccgt
    30121 aatgcaggtc gccttcttca gtctggctgg gggtaaattc aggcgcttta atgccggtga
    30181 ttttcatctt cgggcgtgcc gccggggcaa atggcgcgct acgaaacgcg gaatgagggt
    30241 tatgttcatc accctcgata tacaggttgc cttcaccgca accgcatgtt gtacacatcg
    30301 ctcactcctg gtctatttct atccgccgaa tctgtaaacc gtcgtctgcc acaatctgta
    30361 gcatgtcacc atgacactgt ggacagcggc ggacgcgctg ggtcagtagc gtcacatact
    30421 gttggcatgt ttcacaccag cattcggcct cttgttcttc gaggtgcagt ttacaacctt
    30481 ccgccacgct gccgcggcaa accagatcaa aacaaaaggc aagagagctg gtttcgacac
    30541 aagaaaatgc gccaattttg agccagaccc cagttacgcg ttttgcgccg tgttttgcgg
    30601 cctgctgttc gatcaattcc agtgcccgtt ggcagagggt tatttcgtgc atatcgcctc
    30661 ccattaacta ttgccagcta caagcaataa ttgtgccagt gttgattatc cctgcagtga
    30721 ataatgtcga tgatgtcgaa atgacacgtc gacacggcga cgaaattcac caaaatctta
    30781 aaaatcgctt taataataat aaattaaaag ttggcacaaa aaatgcttaa agctggcatc
    30841 tctgttaaac gggtaacctg acaatgacta tttgggaaat aagcgagaaa gccgattaca
    30901 tcgcacagcg gcatcgtcgc ctacaggacc agtggcacat ctactgcaat tcgctggttc
    30961 aggggatcac gttatcgaaa gcgcgcctgc atcacgccat gagctgcgcg ccggacaaag
    31021 aactctgttt cgtccttttt gaacattttc gtatttacgt caccctggcg gatggcttta
    31081 acagccacac catcgagtat tacgtcgaaa caaaagatgg cgaagacaaa cagcggattg
    31141 cgcaggcgca actgagcatt gacggcatga ttgatggcaa ggtcaacatc cgcgatcgcg
    31201 aacaggttct ggaacactat ctcgaaaaaa tcgctggcgt ttacgacagc ttatacaccg
    31261 ctattgaaaa caatgtgccg gtgaatttaa gccaactggt aaagggacaa agcccggcag
    31321 catgagctga ggctttgccc gttttgcagg cgttacgcct gtttggggat gggcgtgtcg
    31381 atgagtgtcg aaaatgacat ttcatcggca tgttttcgtc aaaaatgaca atcacctgag
    31441 gaatgcctgg tgaatcgttt tgtaattgct gactccacgc tctgtatcgg ttgccacact
    31501 tgtgaggccg cctgttcaga gacgcatcgc cagcacggcc tgcaatcaat gccgcgcctg
    31561 agagtgatgc taaatgaaaa agaatctgcg ccgcagctct gtcaccactg tgaagatgca
    31621 ccctgcgcgg tggtctgccc ggttaacgcc atcacccgcg ttgatggggc cgtgcagttg
    31681 aatgaaagcc tgtgcgtaag ctgcaagctg tgcggcatcg cctgcccgtt tggcgcaatt
    31741 gaattttccg gcagccgtcc gctggatatt cctgcaaatg ccaatacccc gaaagcgcca
    31801 ccggcaccgc ctgctccggc gcgtatcagt acgctgcttg actgggtgcc gggtattcgc
    31861 gcgatcgccg tcaaatgtga cctttgtagc tttgatgaac aaggtccggc ctgcgtgcgg
    31921 atgtgcccga ctaaagccct gcatctggtg gataacaccg atatcgcccg cgtcagcaaa
    31981 cgtaagcgtg agctgacctt taacacggac tttggcgatc tcaccttgtt tcagcaggct
    32041 caaagtggag aggctaaatg agcgcaattt ccctgatcaa tagcggcgtg gcgtggtttg
    32101 tcgccgccgc tgttctggca tttctctttt cttttcaaaa ggcgctaagc ggctggatag
    32161 ccggaattgg cggcgcggtt ggtagtctgt atacggcagc cgcgggcttc actgtactga
    32221 ctggcgcggt tggcgtgagc ggtgcgctgt cgctggtaag ctacgatgtg caaatctctc
    32281 cgcttaacgc gatttggctg attacgctcg gtctgtgcgg tctgtttgtc agcctctaca
    32341 acattgactg gcatcgccac gcgcaggtga agtgcaacgg cttgcagatc aatatgttga
    32401 tggctgccgc cgtctgcgcc gtcattgcca gcaacctcgg catgttcgtg gtaatggccg
    32461 aagtcatggc cttgtgtgcg gtgttcctca ccagcaacag caaagagggc aaactgtggt
    32521 ttgcgctggg gcgtcttggc actctgctgc tggcgattgc ttgctggctg ctgtggcagc
    32581 gttacggcac gctggatctg cgcctgctgg atatgcgtat gcaacagctg ccgctcggtt
    32641 ccgatatctg gctgctcagt gtgattggct ttggcctgct ggccgggatt attccgctgc
    32701 acggctgggt gccgcaggca catgcgaacg cctctgcgcc agctgccgcg ttgttttcta
    32761 cggtagtcat gaaaattggc ctgctgggca ttttaaccct gtcactgctg ggcggtaatg
    32821 caccgctgtg gtgggggatc gcgctgctgg tgctcggtat gatcaccgcg tttgtcggcg
    32881 gtctgtatgc gttgatggaa cataatatcc agcgtctact ggcttaccac accctggaaa
    32941 atatcggcat tatcctgctg gggctgggcg ctggcgtaac gggtatcgcg ctcgaacaac
    33001 cggcgctgat tgctcttggc ctggtcggtg gtctgtacca tctgcttaac catagcctgt
    33061 tcaaaagcgt actgttcctc ggggcgggga gcgtctggtt ccgtaccggt catcgcgata
    33121 tcgaaaaact cggtggtatt ggcaagaaaa tgccggttat ctccatcgct atgttagtcg
    33181 ggctgatggc aatggctgca ctgccgccgc tgaacggttt tgccggggaa tgggttatct
    33241 atcaatcctt cttcaaactg agcaatagtg gcgcgtttgt tgcccgtctg ctggggccgc
    33301 tgctcgctgt gggtctggca attaccggtg cgctggcggt gatgtgtatg gcgaaagtct
    33361 atggcgtcac gttcctcggc gcgccgcgta ccaaagaagc cgaaaacgcc acctgtgcac
    33421 cgctcctgat gagcgtaagc gtagtggcac tggcgatttg ctgcgtaatt ggcggtgttg
    33481 ctgcgccgtg gctgctgccg atgctctctg ctgctgtacc tctgccgctg gaacctgcta
    33541 acaccaccgt ttctcaaccg atgatcacgt tgctgctgat tgcctgcccg ctgctgccat
    33601 tcatcattat ggcgatttgc aaaggcgatc gtttgccatc gcgttcccgc ggcgcggcct
    33661 gggtgtgcgg ctacgaccat gaaaaatcaa tggtaattac cgcccacggt tttgccatgc
    33721 cggtgaaaca ggcgtttgcg ccggtgctga aactgcgcaa atggctgaat ccggtgtcgc
    33781 tggtgccggg ctggcagtgc gaaggcagtg cgttgctgtt ccgccggatg gcgctggttg
    33841 aactggcggt gctggtggtg attattgttt cacgaggagc ctgagaatga gtgttttata
    33901 tccgttaatt caggcgctgg tgctatttgc cgttgcgccg ctgctctccg gtattacccg
    33961 cgtggcgcgc gcccgcttgc ataaccgtcg cgggccgggc gtgttgcagg agtatcgcga
    34021 cattatcaaa ctgctgggtc gccagagcgt tggcccggat gcctccggct gggtgttccg
    34081 cctgacgccg tatgtgatgg tgggcgtcat gctgactatc gctactgcgc tgccggtggt
    34141 gaccgtcggt tctccgctgc cgcaactggg tgatttgatc accttactgt atctctttgc
    34201 catcgcgcgt ttcttctttg ccatttctgg tctggatacc ggtagtccgt ttaccgctat
    34261 cggcgcgagc cgtgaagcga tgcttggcgt gctggtcgaa ccgatgctgc tgcttggtct
    34321 gtgggttgcc gcacaggttg ccagttccac caacatcagc aacatcaccg acaccgttta
    34381 tcactggccg ctgagccaga gcatcccgct ggtgctggcg ctttgtgcct gtgcgttcgc
    34441 cacttttatc gaaatgggca aactgccgtt cgacctggcg gaagccgagc aggagttgca
    34501 ggaaggtccg ctctctgagt acagcggcag cggctttggc gtcatgaaat ggggtatcag
    34561 cctgaaacag ttggtggtgc tgcagatgtt cgtcggggtg tttattccgt ggggacaaat
    34621 ggaaaccttc accgtcggtg ggctgctgct ggcgttggtg attgccatcg tcaaactggt
    34681 ggtcggcgtc ctggttatcg cgctgttcga aaacagcatg gcccgtctgc gtcttgatat
    34741 tactccgcgc attacctggg ctgggtttgg ctttgcattt ttagcgttcg tctccttgct
    34801 ggcggcgtga ttaaagagag tttgagcatg tctgaagaaa aattaggtca acattatctc
    34861 gccgcgctga atgaggcatt tccgggcgtc gtgctggacc acgcctggca gaccaaagat
    34921 cagctgactg tcaccgtaaa ggtgaactat ctgccggaag tggtggagtt tctctactac
    34981 aagcaggggg gctggctgtc ggtgcttttt ggtaacgacg aacgcaaact gaatggtcat
    35041 tacgccgttt actacgtgct gtcgatggag aaggggacta agtgctgggt aacggttcgc
    35101 gtcgaagttg atgccaacaa accggagtat ccttccgtga cgccgcgcgt tccggctgcg
    35161 gtgtggggcg agcgtgaagt acgtgatatg tacggtttga ttccggttgg tttgccggat
    35221 gaacgtcgtc tggtgctgcc ggatgactgg ccggatgaac tttatccgct gcgtaaagac
    35281 agcatggatt atcgtcagcg tccggcaccg accaccgatg ctgaaaccta cgagttcatc
    35341 aacgaactgg gcgacaagaa aaacaacgtc gtgccgattg gtccgctgca cgtcacttct
    35401 gacgaaccgg gccacttccg tctgttcgtc gatggcgaaa acattatcga cgccgactac
    35461 cgcctgttct atgtccatcg cggcatggaa aaactggcag aaacccgcat gggttataac
    35521 gaagtgacct tcctctctga ccgtgtgtgc gggatctgcg gttttgctca cagcaccgcc
    35581 tacaccacgt cggtggaaaa cgcgatgggt attcaggtgc cagaacgtgc gcagatgatc
    35641 cgcgccattc tgctggaggt agaacgtctg cactcgcatc tgctcaacct cggcctcgcc
    35701 tgtcacttta ccggctttga ctccggcttt atgcagttct tccgcgtgcg tgaaacctcc
    35761 atgaaaatgg cagagatcct taccggtgcg cgtaaaacct acggcctgaa cctgatcggc
    35821 gggattcgtc gcgatctgct gaaagatgac atgatccaga cccgtcaact ggcgcaacag
    35881 atgcgtcgtg aagtgcagga gctggtggat gtgctgctga gtacgccgaa catggaacag
    35941 cgcactgtcg gcattggtcg tctggacccg gaaatcgctc gcgacttcag taacgtcggc
    36001 ccgatggtcc gtgccagcgg tcacgcccgc gatacccgcg ccgatcaccc gtttgttggt
    36061 tatggcctgc tgccaatgga agtccacagc gagcagggct gcgacgttat ttcgcgtctg
    36121 aaagtgcgta ttaacgaagt ctataccgcg ctgaacatga tcgactacgg tctggataac
    36181 ctgccgggcg gcccgctgat ggtggaaggc tttacctaca ttccgcaccg ttttgcgctg
    36241 ggctttgccg aagcgccgcg cggcgatgat atccactgga gcatgaccgg cgacaaccag
    36301 aagctgtacc gctggcgctg ccgtgccgcg acctacgcga actggccgac cctgcgctac
    36361 atgctgcgcg gcaacaccgt ttctgatgcg ccgctgatta tcggtagtct ggacccttgc
    36421 tactcctgta ccgaccgcat gaccgtggtc gatgtgcgta agaaaaagag caaagtggtg
    36481 ccgtacaaag aactcgagcg ttacagcatt gagcgtaaaa actcgccgct gaaataagga
    36541 atcgccatgt ttacctttat caaaaaagtc atcaaaaccg gcacggcgac ctcgtcttat
    36601 ccgctggaac cgattgcggt tgataaaaac ttccgtggta agccagagca gaacccgcag
    36661 cagtgcatcg gctgcgcggc ctgcgtcaat gcctgcccgt caaacgcctt aacggttgaa
    36721 actgacctcg ccaccggtga gctggcgtgg gagttcaatc tcgggcgctg catcttctgc
    36781 ggacgctgcg aagaagtctg cccgacggcg gcaatcaaac tgtcgcaaga gtacgaactg
    36841 gcggtgtgga agaaagaaga cttcctgcaa cagtcccgct tcgcgctgtg caactgccgc
    36901 gtctgcaatc gtcctttcgc cgtccagaaa gagatcgact acgccattgc gctgcttaag
    36961 cacaatggcg acagccgcgc ggaaaaccac cgcgaaagct ttgagacttg cccggaatgt
    37021 aagcgccaga aatgcctggt gccgtccgac cgtattgaac tgactcgcca tatgaaagag
    37081 gccatctgat gagcaattta ttaggccccc gtgacgccaa cggcattccg gtcccgatga
    37141 cggtggatga atccatcgcc agcatgaagg cgtcgttact gaaaaaaatc aaacgttctg
    37201 cctacgttta ccgcgtggac tgcggcggct gcaacggctg cgaaatcgaa attttcgcca
    37261 ctttgtcgcc gctgttcgat gcagaacgct tcggcattaa agtcgttcct tcaccgcgtc
    37321 atgcggatat tttactgttt accggcgcgg tcacccgtgc aatgcgatcc cctgcgctgc
    37381 gtgcgtggca gtccgcgccg gacccgaaaa tttgtatctc ctacggtgcc tgcggtaaca
    37441 gcggcgggat cttccacgat ctctactgcg tgtggggcgg tacggataaa atcgtcccgg
    37501 tggatgttta tatccctggc tgcccgccaa cgccagccgc cacgctgtac ggctttgcaa
    37561 tggcgctcgg cctgctggag cagaaaattc acgcccgtgg gccgggtgag caggatgaac
    37621 aaccggcgga gatcctgcat ggcgatatgg tgcagccgct gcgtgtgaaa gtggatcgcg
    37681 aagcacgtcg actggcgggt tatcgttacg gtcgtcagat tgccgatgat taccttacac
    37741 agttagggca gggcgaagaa caggttgcac gctggctgga agcggaaaac gatccgcgtc
    37801 tgaacgagat tgtcagccat ctgaatcatg ttgttgaaga ggcgcgtatc cgatgagtga
    37861 aaaggtggtg ttcagtcaac tgagccgtaa atttattgat gagaacgatg ccacgcccgc
    37921 cgaggcgcag caggtggtct attacagcct ggcgattggt caccaccttg gggttatcga
    37981 ttgcctggaa gcggcgctca cctgcccgtg ggatgaatat ctggcatgga ttgccactct
    38041 ggaggcgggc agcgaagccc gccgcaaaat ggaaggtgtg ccgaaatatg gtgagatcgt
    38101 catcgacatt aaccatgtgc cgatgctggc caatgcattc gataaagccc gggcagcgca
    38161 aacttcgcag cagcaggaat ggagtacaat gctgttaagt atgctgcatg atattcatca
    38221 ggaaaacgcc atctatttga tggtgaggag actgcgtgac tgacgtttta ctctgtgttg
    38281 gcaatagcat gatgggtgat gatggcgcag gtccgctgct ggcggaaaag tgcgccgccg
    38341 cgccgaaagg taactgggtg gtgattgacg gcggtagcgc accggaaaac gacattgtcg
    38401 ctatccgcga actgcgcccg acacggctgc tgattgtcga cgccacggat atggggctaa
    38461 accccggcga aatccgcatc atcgacccgg atgatatcgc cgagatgttt atgatgacta
    38521 cccataacat gccgttgaac taccttatcg atcagttgaa agaagatatt ggcgaagtga
    38581 ttttcctcgg catccagccg gatatcgtcg gcttttacta cccgatgacc cagccgatta
    38641 aagatgcggt agaaaccgtt tatcaacgac tggaaggctg ggaagggaac ggcggcttcg
    38701 cgcagttagc ggtggaagaa gagtagtttt tcaataagta atcaggacag ggatgttctt
    38761 gatgagggaa tcagaactga tagcaaaccc cagattgccc gatgcgcaac gcttatcggg
    38821 ccttcctggt tagtgcgaca gtaggttacc tactctaaat cttccccatt actggcaatc
    38881 acttttttat accaccagaa cgatttctta cgtgtgcgcg tcagcgtgcc gttgcctgcg
    38941 tcgtcacggt caacaaagac aaaaccgtag cgtttgctca tttcacccgt agaggcggaa
    39001 actaaatcaa tacagcccca tgtggtgtag cccatcagcg gaatgccatc tgcaatcgct
    39061 tcgcccattg cgcggatatg ttcgcgtaaa tagctgatgc gatagtcatc gttaatctcg
    39121 ccattggcag caagttcatc ttttgcgccg aggccgtttt ccaccagaaa cagcggcttc
    39181 tgatagcggt cgtacatcat gttcatggtg atacgcagac cgagtggatc aattccccag
    39241 ccccagtcgc tcacctgtag atacgggtta cgcagcgatt tcactacgtt cgccgcactg
    39301 ctattgttgg tgttcatctc cgccgaggcg cagcgcgagg cgtaatagct gaaagagaca
    39361 aaatcgacgg tgtttttcag gatctcatca tcgcccggtg ctttgtcgat ggttacccct
    39421 ttttcgcgga atacgcgagc agagtaagcc ggatacgcgc cccgcgcctg cacatcgata
    39481 aaaaacaggt tttcccgatc tttctccagc gccgcccaga catcttccgg cttgcaactg
    39541 taagggtaga agttaccgcc cgccagcata cagcccacct gattttgcgg gttaacctca
    39601 tgggcgattt tggtggctag cgcactggca accagctggt gatgcgcggc ctgatatttc
    39661 acctgatcct gattttcacc ttcttcaaac accagccccg cgccggagaa cgggctatgc
    39721 aacataatgt tgatttcatt gaaggttagc cagtatttca ccagaccatc aaatgcttca
    39781 aagcaggtcc tggcgtagcg gctgaaaaac tccaccagct tgcggttacg ccaggagcca
    39841 tattcggtga ccagctgcat cggcacatcg aagtggcaca atgtgaccag cggttcgata
    39901 ccgtactttt tacactcttc aaagacagaa cggtaaaaag caatgccctg ctggttgggc
    39961 gtcagttcat caccctgcgg gaaggggcgg ctccaggcaa ttgaggtacg gaacactttg
    40021 aatcccatct ctgccatcag ggcgatatct tctttataac gatgataaaa acccgtcgcc
    40081 tcatggctgg gataaaactc gtcatcgcgc aactgaaaac gtttttccag ccccagtttc
    40141 accgccattc gatgctcgcc gtgtgggatc atatcgaccg ttgtcagtcc tttgccacct
    40201 tcacggaacg caccttcaga ctggttggcg gcaagcgcgc cgccccataa aaaaccttct
    40261 ggaaatactg acattttcat cctcaattaa gacttacttc tttggcgacg gtcggttgtg
    40321 cgctctgatg ctttcgcgcc tcggcagccg cttcctcaac aggaatatcc tcaaagccga
    40381 gcaacagtgt gaggataaac gacagcacca ccgccagcgc catgaccgcg aacacccaga
    40441 cgatgctcat tggattcgcc ggatcgaaga actgcacgct ggtaaatagc cccggcgctg
    40501 ccattgagtg gctggcaagc cccgccatac cggcaaccgc gccgcaaata aaaccgctga
    40561 taagactggc gataagcgga cgtttcaggc ggatcgccac gccgtataac gccggttcgg
    40621 aaatccccgc cataatggct gatgccgccg cagccagcgc agtctggcgc agttccgagt
    40681 ttttcgtttt ccacgccacc gccagtgatg aaccgcccag cgacaggtta gcgccgatct
    40741 ctgacggcat gaccatccct tctttgccgg tttcggcaat ggtctgaatg atggttagcg
    40801 taaagacgcg gtgcatcccg gtcattacca gcagaggcca cagcgcgccc ataatggcga
    40861 ctgaaagcca gcccagataa ccatgaatgg tgtaaaccag cgccgaaatg gcgctaccga
    40921 tccagatacc aatcgggcca atcagcagga ttgccagcgg tgcggcaatc aacacaatca
    40981 gcattggctt gaggaagttt ttggtcaccg ccggtgtaat gcgatccacc cagcgttcga
    41041 tatatgacag gcaccaggtc atcaccagcg ccgggatcac tgtgtaggtg tatttcaccg
    41101 cggtgactgg aatcagggca aattcgacat gttcaccctg ggccgctttc gccatcagtt
    41161 caataaaact cggatgtacc agcacacccg cgatggcaat cgccagcgac atattggttt
    41221 taaatttgat ggcggccgaa gccgcgacca tcagcggcag gaagaagaaa gcaccgtcac
    41281 caatcacatt cagaatggtt aaggtcggcg atccttttgt cagcacgccg ctcatctcga
    41341 ggatcatcgc cagcagtttg accatcgatc cgccgataat cgccgggatc agcggtgaca
    41401 tggtgccgat cagcgcatcg aggatccccg caccaatgcg acgcagcgtg agtttgggtt
    41461 tacccacggg cagggcgggc tgcatatctc ccggcagcag gctgacgact tcctgaaagg
    41521 cttgtgaaac ggtattgcca ataatcacct gacactggtt gtcactacgt accacgccga
    41581 gcacgccggg gatggttttt aacgtcgggc tgtcgataag cgcatcatct ttgataacaa
    41641 agcgcaaacg cgtcatacag tgcgtgaccg ccgagatgtt atcaacgccg cccagtgccg
    41701 ctatcaccga gcgtgccagc gccgcataat ttttggccat cggattttat cctgttatca
    41761 gtagggaaga tgtagttgtt atcagtcgac gtgacggaaa aatgccgatg ctgaaacgga
    41821 attaagaaac cggtttcaca aaatca
//
LOCUS       DBFIFM010000014        40199 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.014, whole genome shotgun sequence.
ACCESSION   DBFIFM010000014 DBFIFM010000000
VERSION     DBFIFM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.014"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(48..1907)
                     /gene="gss"
                     /locus_tag="N7C74_000714"
     CDS             complement(48..1907)
                     /gene="gss"
                     /locus_tag="N7C74_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HGU2430651.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2112..2978
                     /gene="yghU"
                     /locus_tag="N7C74_000715"
     CDS             2112..2978
                     /gene="yghU"
                     /locus_tag="N7C74_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HGU2430652.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3101..3349)
                     /gene="hybG"
                     /locus_tag="N7C74_000716"
     CDS             complement(3101..3349)
                     /gene="hybG"
                     /locus_tag="N7C74_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HGU2430653.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3362..3703)
                     /gene="hypA"
                     /locus_tag="N7C74_000717"
     CDS             complement(3362..3703)
                     /gene="hypA"
                     /locus_tag="N7C74_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HGU2430654.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEVHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3696..4184)
                     /gene="hybE"
                     /locus_tag="N7C74_000718"
     CDS             complement(3696..4184)
                     /gene="hybE"
                     /locus_tag="N7C74_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HGU2430655.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHALSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4177..4671)
                     /gene="hybD"
                     /locus_tag="N7C74_000719"
     CDS             complement(4177..4671)
                     /gene="hybD"
                     /locus_tag="N7C74_000719"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HGU2430656.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4671..6374)
                     /gene="hybC"
                     /locus_tag="N7C74_000720"
     CDS             complement(4671..6374)
                     /gene="hybC"
                     /locus_tag="N7C74_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HGU2430657.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6371..7549)
                     /gene="hybB"
                     /locus_tag="N7C74_000721"
     CDS             complement(6371..7549)
                     /gene="hybB"
                     /locus_tag="N7C74_000721"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HGU2430658.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7539..8525)
                     /gene="hybA"
                     /locus_tag="N7C74_000722"
     CDS             complement(7539..8525)
                     /gene="hybA"
                     /locus_tag="N7C74_000722"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HGU2430659.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8528..9646)
                     /gene="hybO"
                     /locus_tag="N7C74_000723"
     CDS             complement(8528..9646)
                     /gene="hybO"
                     /locus_tag="N7C74_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HGU2430660.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9835..10122)
                     /gene="yghW"
                     /locus_tag="N7C74_000724"
     CDS             complement(9835..10122)
                     /gene="yghW"
                     /locus_tag="N7C74_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HGU2430661.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10241..11128)
                     /gene="yghX"
                     /locus_tag="N7C74_000725"
     CDS             complement(10241..11128)
                     /gene="yghX"
                     /locus_tag="N7C74_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HGU2430662.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIFAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLNSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRILKWFDKYLS"
     gene            11285..12325
                     /gene="gpr"
                     /locus_tag="N7C74_000726"
     CDS             11285..12325
                     /gene="gpr"
                     /locus_tag="N7C74_000726"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HGU2430663.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPEDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLVGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12365..12859)
                     /gene="yqhA"
                     /locus_tag="N7C74_000727"
     CDS             complement(12365..12859)
                     /gene="yqhA"
                     /locus_tag="N7C74_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HGU2430664.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13050..13934
                     /gene="yghA"
                     /locus_tag="N7C74_000728"
     CDS             13050..13934
                     /gene="yghA"
                     /locus_tag="N7C74_000728"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HGU2430665.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14206..14631)
                     /gene="exbD"
                     /locus_tag="N7C74_000729"
     CDS             complement(14206..14631)
                     /gene="exbD"
                     /locus_tag="N7C74_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HGU2430666.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14638..15372)
                     /gene="exbB"
                     /locus_tag="N7C74_000730"
     CDS             complement(14638..15372)
                     /gene="exbB"
                     /locus_tag="N7C74_000730"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HGU2430667.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15624..16811
                     /gene="metC"
                     /locus_tag="N7C74_000731"
     CDS             15624..16811
                     /gene="metC"
                     /locus_tag="N7C74_000731"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HGU2430668.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16951..17610
                     /gene="yghB"
                     /locus_tag="N7C74_000732"
     CDS             16951..17610
                     /gene="yghB"
                     /locus_tag="N7C74_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HGU2430669.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17650..18549)
                     /gene="yqhC"
                     /locus_tag="N7C74_000733"
     CDS             complement(17650..18549)
                     /gene="yqhC"
                     /locus_tag="N7C74_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HGU2430670.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMARPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18743..19906
                     /gene="yqhD"
                     /locus_tag="N7C74_000734"
     CDS             18743..19906
                     /gene="yqhD"
                     /locus_tag="N7C74_000734"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311921.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HGU2430671.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLGVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            20011..20838
                     /gene="dkgA"
                     /locus_tag="N7C74_000735"
     CDS             20011..20838
                     /gene="dkgA"
                     /locus_tag="N7C74_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HGU2430672.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     FDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHNRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21038..21960
                     /gene="yqhG"
                     /locus_tag="N7C74_000736"
                     /pseudo
     CDS             21038..21960
                     /gene="yqhG"
                     /locus_tag="N7C74_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
     gene            22009..22266
                     /gene="yqhH"
                     /locus_tag="N7C74_000737"
     CDS             22009..22266
                     /gene="yqhH"
                     /locus_tag="N7C74_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HGU2430673.1"
                     /translation="MKTIFTVGAVVLATCVLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22309..24528)
                     /gene="ygiQ"
                     /locus_tag="N7C74_000738"
     CDS             complement(22309..24528)
                     /gene="ygiQ"
                     /locus_tag="N7C74_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HGU2430674.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQCQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24639..26051)
                     /gene="ftsP"
                     /locus_tag="N7C74_000739"
     CDS             complement(24639..26051)
                     /gene="ftsP"
                     /locus_tag="N7C74_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HGU2430675.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26126..26863)
                     /gene="plsC"
                     /locus_tag="N7C74_000740"
                     /gene_synonym="parF"
     CDS             complement(26126..26863)
                     /gene="plsC"
                     /locus_tag="N7C74_000740"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HGU2430676.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27097..29355)
                     /gene="parC"
                     /locus_tag="N7C74_000741"
     CDS             complement(27097..29355)
                     /gene="parC"
                     /locus_tag="N7C74_000741"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HGU2430677.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29493..31100)
                     /gene="ygiS"
                     /locus_tag="N7C74_000742"
     CDS             complement(29493..31100)
                     /gene="ygiS"
                     /locus_tag="N7C74_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU2430678.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPTNTQLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGRRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGEIRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQAMQITDATKRNALYQQAEVIVNQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(31209..31691)
                     /gene="ygiV"
                     /locus_tag="N7C74_000743"
     CDS             complement(31209..31691)
                     /gene="ygiV"
                     /locus_tag="N7C74_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HGU2430679.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCRPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETNVYVPLA
                     "
     gene            complement(31744..32136)
                     /gene="ygiW"
                     /locus_tag="N7C74_000744"
     CDS             complement(31744..32136)
                     /gene="ygiW"
                     /locus_tag="N7C74_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HGU2430680.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            32288..32947
                     /gene="qseB"
                     /locus_tag="N7C74_000745"
     CDS             32288..32947
                     /gene="qseB"
                     /locus_tag="N7C74_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HGU2430681.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            32944..34293
                     /gene="qseC"
                     /locus_tag="N7C74_000746"
     CDS             32944..34293
                     /gene="qseC"
                     /locus_tag="N7C74_000746"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HGU2430682.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPVMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            34403..34984
                     /gene="mdaB"
                     /locus_tag="N7C74_000747"
     CDS             34403..34984
                     /gene="mdaB"
                     /locus_tag="N7C74_000747"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HGU2430683.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            35015..35329
                     /gene="ygiN"
                     /locus_tag="N7C74_000748"
     CDS             35015..35329
                     /gene="ygiN"
                     /locus_tag="N7C74_000748"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HGU2430684.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(35374..36261)
                     /locus_tag="N7C74_000749"
     CDS             complement(35374..36261)
                     /locus_tag="N7C74_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGU2430685.1"
                     /translation="MIQKDKTRVDIYGERFRTRASQLTPGLRAVASYINEHREVVLDQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(36258..37205)
                     /locus_tag="N7C74_000750"
     CDS             complement(36258..37205)
                     /locus_tag="N7C74_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HGU2430686.1"
                     /translation="MRLFFSLLILLSFFARATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIERLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(37216..38253)
                     /locus_tag="N7C74_000751"
     CDS             complement(37216..38253)
                     /locus_tag="N7C74_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HGU2430687.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLVLLGGLFLFAVGILIFGLMHGS
                     FFVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCAREISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(38262..39245)
                     /locus_tag="N7C74_000752"
     CDS             complement(38262..39245)
                     /locus_tag="N7C74_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HGU2430688.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFEP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGVVLAIYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(39242..40051)
                     /locus_tag="N7C74_000753"
     CDS             complement(39242..40051)
                     /locus_tag="N7C74_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2430689.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARVLQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINTLTGKPFFMPFRGVEKV"
BASE COUNT        10036 a        10797 c        10075 g         9291 t
ORIGIN      
        1 cgtactgact attcttcata atcagtacgg gattgctaca acatgtatta ctctttcacc
       61 acaattaacg gttcaatatc actctctttt ttaataacca gtgattcatc accgcgcaaa
      121 cacgtcccac cgtagttgcc gccaacggtg aaggtacata cctgaatgta tttaccgtcc
      181 actttcggca aacaccacag ttgctgatag atgtttttct gctcggcaaa tttaccactg
      241 gttttgtcca gcacctcttc atgatggctg acgaggtcga tgttgctgcc acagcgaccg
      301 gcgatcggtt tcactgcgta acctgttttc accaattcat cattaacagt gaaatcggta
      361 tccagcaggt aacgatggtg cgggaacagc gaccagagga tcggcagaat cgctttgttg
      421 ccggggatca ccgtccacag cggctcaaag accagcactt ccgggcgcag caatacgtcg
      481 ataagacgca cttcattttg cggatgaccg gtacggatcg gcaccgcagc aaactcacgg
      541 tcgctaactt cacgaatctg atcaaacgcg gtttcccacg cccaggtttt ccacacgcag
      601 ttaaccagtc gcccttcccc atcaatcagt tgcccggcag catcccagcc cagttcatcc
      661 agcccgcgca agatacgcgt ttcaaagccc gcctggtgca gcgcctgctc cataaactgc
      721 gcgtgatagt tttcctcgat atctttgtcc tgcatgatat ggacaaacgg acgtgcacga
      781 ctgtgtttcc aggcaccagc cagttcgtta atcagccctt ccgccggatt gaagccgttg
      841 cctttatagc cctgctccgc ccaacgttcg aggatcaagc ccgcttcggt atgacaggag
      901 gcggagtcgg cgttgtactc gtaaaccttc agaccacgct catccatgca gaaatccata
      961 cgaccggtga tcatatggtg acggcgacgc tgccaggaga gacgcaaacg tggccagagg
     1021 attttcggaa tgtcgaacag cgccagcagg ttgtcatctt tcagcacctt gtcggttgcg
     1081 tgaagataca tcaggtgcag ctcgttggtg gctttaatca gctcctgctc ggcgctctcg
     1141 gtaatggtgt agtaatgata cggatcctga ttgatcacct gaccgttggc ctgcacatag
     1201 gcgttttgca gcggatcttt ttcatccagc catttaccgt caaactggcc tttgttttcc
     1261 agacgcgctc cgctgatttt cagcagctcg cctgcaattt ccggctgcgg taagctgtat
     1321 tcagtatctt ccgtctggat catccagccc agaatggtgg tgtcatcaaa agtgtcttta
     1381 agggtatagc agccgttttc gaccaccatc tccagttcgc gcgtccactg ttgcccttgc
     1441 ggcaacgggg aatgaatcac gttctgttcc gcaatacgga ctttgttgcc atgtaattgg
     1501 gtaatgatgg cgacatggcc agtgtcttta aattcaccgc ctttatccca gataagaagc
     1561 gcacccgcga ccggcgcacg cggcgagccg ttaggaaatg cctgcaatgg caggatgttg
     1621 tcattaacca cttcacgcag gaagcgcagc gagaaaatct cccacgccat acccacgtca
     1681 gtaaagacca caccgtagtt cagaaagaga aaacggcgag caaattcaac gcattgccat
     1741 ttgtggccca tatattcgtc gtcgatatag ctacggaata cggcgtcatc ttcgtattcc
     1801 tgcggatcga gagaactgta atctgaagag tagattgcta ccccacctgg ggcgtagccc
     1861 aataatgtcc cgaacggggc atcctggctg gtcgttcctt tgctcatcac tttaccttta
     1921 acaatacaac ctaagcagtt ggcgtaattg ttgtgctctg attacctgct cagcgacatt
     1981 aaccggacag aggtcagact aatcatacac ctcatcgcag cggctggcgc aaagggttaa
     2041 aaaattcaca ttctgtacag caagtgacct gctacactgc ttcaacacta ccactcagaa
     2101 ggcaactcac tatgacagac aatacttatc agcccgcgaa agtctggacg tgggataaat
     2161 ccgctggcgg cgcgttcgcc aatatcaatc gcccggtttc tggtccgacg catgaaaaaa
     2221 cgctgccggt gggcaaacat ccattacaac tttattcgct gggaacgccg aacggtcaga
     2281 aagtaacgat tatgcttgag gagctgctcg cgctgggcgt tactggcgca gagtacgatg
     2341 cctggctgat acgcataggc gatggtgatc aattctccag cggctttgtc gaagtgaacc
     2401 caaattcgaa gatcccggcg ctgcgcgatc atacccataa tccgccgatc cgcgtgtttg
     2461 agtcaggatc gattctgctt tatctggcgg agaaatttgg ctacttcctg ccgcaggatc
     2521 tggcaaaacg gactgaaacg atgaactggc tgttctggtt acagggcgcg gcaccgttcc
     2581 tcggcggtgg ttttggtcac ttttaccatt acgcgccggt gaaaattgag tacgccatca
     2641 atcgctttac catggaagcc aagcgcctgc tcgacgtgct ggataagcaa ctggcgcagc
     2701 ataagtttgt tgcgggcgat gagtacacca ttgcggatat ggcgatttgg ccgtggtttg
     2761 gcaacgtggt gctcggcggc gtgtatgatg ccgcagagtt tctggatgcg ggaagttata
     2821 aacatgtgca acgctgggcg aaagaagtgg gcgaacgtcc ggcagtgaag cgtgggcgta
     2881 ttgttaaccg caccaacggg ccgctgaacg agcagttgca tgagcgccat gacgccagtg
     2941 attttgagac gaatacggaa gataagcgtc aggggtaagg attggtgttc gtcgcagcaa
     3001 gccatccagg ccggataagg cgttcacgcc gcatccggca atcgagcaca atgcctgatg
     3061 cgacgctgcc gcgtcttatc agacctacga aagctaatca tcacgcgctg gtaatgtcgt
     3121 aatccatttg gcgcagtgcg tctaatgtgg ctttggcttc atcttcgtcg atgatgctca
     3181 tggcaaatcc gacgtgtacc agcacccact ggcccagcag atcggcaggg ttaccttcac
     3241 aaatcagggc gatattcacg tcgcgcttga taccacatac ttcaacctgc gcaagctggt
     3301 gaatatcttc accgacagcc agcacctggc ctggaacgcc aatacacatt gttaactccg
     3361 gttattcaac ttcaatactt ttgacgatca gcgaatcgcc ggtatcgaca cgcaaccgct
     3421 cgccgtgaca gagcggacac tgcgcatcgt gctgatgaac ctccaccacc tggctgcaat
     3481 cccagcacca ggcctgggcg ggtttataga cgatatgtaa atcgcaccct tgcgccaccg
     3541 ttccgtggca gacaatttca aaactaaaac ggacggcgct ctcctcaacg caggagagcg
     3601 cgccaatttc cagccacacg gcggtgacgc gcttaacatc gtgctgctcc gcctgccgct
     3661 ggataatttc aacggcgctc tggcaaagcg acaactcatg cattttcgcc actcctgcga
     3721 ccaaacagca gggcgcgacg ccctgcgtgt ggtacatccg gattcgtgac tggcagcgaa
     3781 aggatcattc gtgcgcagtc atccgtcagg cgttggccct cttcaatcga catactgtgc
     3841 gaaagcggtg acatcagcga acaggagagg tattgcgaaa caccgtccag ttcgccaacg
     3901 gtaaaggtca tggtgccata cggcagttgc agaccaattt tttcactcac tttgcgcagc
     3961 ggccagagtt gatccgggcc ggggaagata actgcactca gcatccacgg ggtgatcaca
     4021 caccccgtcc actgaccttc gaacagcgta aaatcagaaa catacaccgg cattgaagga
     4081 tgcagaaaag agagagcgtg catcgaacgc cgggcaattt cttcaaacgc tgcctgtact
     4141 tgcgccttcg gggaggtctg gaaacctgct atctcttcag tcatgaatcg cctcccgtgg
     4201 gatggcttcc acgccagatt cacgcagcgc agccagaacc tgctcaagcg caggttcaat
     4261 cattgcttca accgtcggcg ttaagccgat gtgtggctcc agcgattccg gaattacgcc
     4321 gaccagggtc agctttttcg gaaactcgcc ggtgaagcgc agggccgaca agacgtcggc
     4381 caggccaagc tgatgcggag agattttgtt ggtaaacaac gccggaactt cttcatcccg
     4441 caggatcatc atcgttcccg gcgcgctctt cttcgacaca atggcatccg caataatcag
     4501 atgatcgcga tttgccatgt cgccaagcag ctccattccc gccgtgccgc catcgaggat
     4561 ctcaacataa tccggcagaa tgtatcgttg ctctaacgct tcgacaatcc gcacaccgat
     4621 ggcttcatcg gtcagcaaaa tattgccgac ccctaagact aaaatacgca ttacagaacc
     4681 ttcactgaaa ccacttcgtt gccgtcagca tccactacgt gtaccgcaca ggccatgcac
     4741 gggtcgaagg agtgaatggt acgcaccact tccagcggtt tattcggatc agcaaccggt
     4801 gtacccacca gcgactgctc gtaaggaccg acgtcatcat tgaagttacg cgggccagag
     4861 ttccaggttg atggaacaac tgcctggtag ttgctgatga taccgtcttt gatcaccatc
     4921 cagtgagaga gcataccgcg cggcgcttcg aggaagccaa cacctttgaa ctcacccgtt
     4981 gccggaatgt tcggcttcac aaaggtggtg tgatcgcctt tgccgatatt ggtgatcagt
     5041 gcactgtatt ggttttgcag gatatcctgc aattcacagc agtgaacggt acgaccaata
     5101 atacggccca gcgtggagtg cagctgtgcc acttccagcg tgttgccagt cagtttctga
     5161 taaatcgcaa cgatttcatt cagtttgttc tgggtagatt cgcgacctgc cgccagtttc
     5221 accagcatat tagccagcgg acccacttct accgttttgc cgtagaaagt cggtgatttc
     5281 acccaggaat atttcccgtc gtcagaccaa ccatcataag ccggaatggt ggtaccttcc
     5341 cacggtgcct gcggcgcttc gtctttatac caggagtgct tcgcgctttc ctgaatgcct
     5401 ttgatcaggt attcatcgga atgagaagtg atcggacgat acgaggacag atccgcattc
     5461 tcaatgtagc cgcccgggaa caggaagctg ccgtttttac tgtcggtcgg gaattccggc
     5521 acgctcaggt agttcaccgc acctttaccg cgcgtcagcc attccgggta gaacgcagcg
     5581 ataaccgcag tatcgacctt gtaaacctgc tcaacaaagt cactcagctt gtcgatgaaa
     5641 gacttgatgt acatcaggcg ctcaaggttc agcacgccca gaccgtcgag gttgattggg
     5701 ttagcgaccc cacctaccgc caggttctga atgtgcggcg ttttaccgcc cagcagcgcc
     5761 acgacgcggt tagcgtcacg ctggcactcc agcgcctgca ggtagtgcgc taccgcaatc
     5821 aggttcactt ccggcggcag tttcatcgcc gggtgacccc agtagccatt ggcgaaaata
     5881 cccaactgac cgctggcaac cagatctttg atcttgttct gaactttggt gaactcttcc
     5941 ggactgttca ggtgccaggt cgaaacacct ttcagcattt cggaggcttt ggttgggtca
     6001 gcttgcagtg cagaagtgat gtccacccag tccagcgccg aaagctgata gaaatgaacg
     6061 atatggtcat gcgtggtgtg cgcagccaga atgatgttac ggatgtattg cgcgttaacc
     6121 ggaacgtcga tattcagcgc actttctgcc gcacgaacgg aagacagcgc gtgagtggta
     6181 gtacatacgc cacagatacg ttgcacaatc atccatgcat cgcgcggatc gcggtttttc
     6241 acgatctctt ccatgccgcg ccacatggta ccggaagccc atgctttcga aacgacgcca
     6301 ttttcgattt cgcaatcgat gcgtaaatgc ccctcaatac gggttaccgg atcaatagta
     6361 attctctggc tcatgctttg ctcgcctcat gacgattatg atcgttttgt tttaaaggag
     6421 gaagtatcgg cagtagacga atgagtacga tgtaagcgca aatctcaata gccacaaaac
     6481 caatagaaat caacagttct tcccaggtcg ggaagtaggc gtaaccgccg cccgggttga
     6541 atgccaccag cgaataggtc agacgccagg ttgcacaacc taacagcgcg ctcagtgctg
     6601 acaggaacag catgcgggaa tcattacgca gcttcgccac acgcagaacg accagcggga
     6661 agagcatcag caggacttca atccagaaca tcacggagta gaagtcaccg gcaaacgcta
     6721 acgacagctt gtcgcgatag ataagctcgc caaagcgcag cacgatgaaa atcgccagca
     6781 acacactgat ggtgttggtc agcttgacga ataggctctt ttcatccgga ccgttgccac
     6841 gcagacccgc ctgcaccagt gaaccttcaa agatgacaat cgagaagccc atgatgaacg
     6901 ccgtcagcag cgagaacagc ggcaacattt catagctctg ccacagcgga tgcaccttgt
     6961 agcccgccga gatcatcagc gaccccattg aagactggtg catggtcggc agcagcgcac
     7021 cgagcgcgat gatgaagaac atcaccttgt tcagacgctg tagcgacacc ttccagccca
     7081 gacgttcaaa cagtgccgga gcaaactcca gagccatcac gccgatgtag atggtcatac
     7141 agaccgccgt ctcgaacagt accgagttca cgttgaagtg acccggaatg tagaagtacg
     7201 gcaggttcca gtagcgaccc acgtcgatag tgatcgacaa gccacccagt gagtaaccaa
     7261 acagactcgc caacagcgcc ggacgcacca gcggatggta ttgcccacgg ttaaagacgt
     7321 ataccgccca cgcaagcgcc cagccgccac aggcaaagcc ggtgccaatc agcaggtcaa
     7381 acgcgatcca cacgccccac gggaagccgc cgttcaggtc agagacagag cccagaccga
     7441 acaccagacg cttcacaatc aggagcatac agatgacgat taacggtcca aaaatcatga
     7501 ccggtttact gatgattttg ccgcccagcg gttgtggatc atgactcatg atcgtctcct
     7561 ccgtcgtgat ggtcgttttt ggtgttgcga cgaaccagca cggtcaagcc cgccagcaca
     7621 gccagtggta gcatcatgcc tttatacagg gtgtgttgaa tattttcgga acgcgcaccg
     7681 gtagaaagat cgtccagttt cggcaggtcg agattttcat aaggcacacc cgtcagtacc
     7741 agaacctgag taccgccgcc ctctttctcg ccgtacagat gcggataata tttcggcacc
     7801 gtatgcaggt aagtgtcgcc agatttcagc gtctgacgcg gatagtggta ttcgctgcca
     7861 ggcttcagcg ccagacgttt tttcgcctcc gccatcagct cttcacgcgt accaaaaatc
     7921 accgcgcccg ccgggcacac ttctacgcag ccaggcagac cgcctttatc gagacgttcc
     7981 acacctttct ggttgcacag ctcgcactta tgcagcgcac caaacgggtt gttgtagtcg
     8041 tacttcggca cgttgtacgg acaggcgacc atgcagtaac ggcaaccggt gcacacgtct
     8101 ttgtcgtaat ggacaatgcc ggttttcgga tcttttttca gtgcagagac cgggcacaca
     8161 gagacacagt tcggatcgac gcagtgcata cactgtttct taatgtacgc gtagccgttc
     8221 tcctcctggt ctttgttgac ccctgtgccg ctggtccaca cttgaatgat gttattggta
     8281 tacggcgaca gtttgtcgtt gttcgaccag gtctgttccc cttgcgggtt acgttcaggg
     8341 aagttgatat cctgacactt ggtgacgcaa gcctggcagc ctacgcacaa ggtcgagtcg
     8401 tacaacatcc ccagcgatcc cggaattggc gggcggtttt cagcagccgc atgactgaca
     8461 gacggcagcg cgcccgtcag caatgccccg caggaggctg ctttaataaa attacgtctg
     8521 ttcacggtta ttctccccgt gagtcagcgt tatctttctt ttgctgacga cccagttcac
     8581 gcaccgccat cacgctgaca ccggcaacca gcccaaccac accgccgagc aaaccaatag
     8641 cgcctgcaga gacgttgccg ccctctttag cgttaacatc cggtttctgt gaacgcggag
     8701 tctgattttc gacgttggca agctgatgga tgcctttatg gaagccgata ccttcttcgt
     8761 tacagccata gcacgggtga ccaatcgcca ccggccacac accgccaaca tcgcagaatt
     8821 gcagcgttga gcagttgccg taagtttctg gccctttaca gccgaggtgg tacaggcacc
     8881 agccttcgcg gtggccttca tcaccgaact ctttggcaaa acgaccagca tcgaagtgcg
     8941 ggcgacgttc gcagtgttcg tgaatcagac ggccataggc gaaggtcgga cggtttttgt
     9001 catccagttt cggcggtttg ccgtaagtga tgatgtgcgc aacggtcgcg aggaagttgt
     9061 gcgggttcgg cgggcagccc ggaatattga taacggtttt gcctggcaga acttcttgca
     9121 agctgactgc gccagttggg ttaactccag ctgcggcaac accgccccac gcagagcagg
     9181 aaccgatagc gataatggct gctgcgcctt ccgccgcttt gcggatgtga tccacaatcg
     9241 gctcaccggc aaccatgcaa taaataccgt tatcttttaa tgggatggaa ccatccacca
     9301 ctaacacata ctgccctttg tacttctcga gagcgttatg tttgttctct tcgacctgat
     9361 gaccgaaggc ggcggaaagc acttcgtgat actccagaga gatagtctcc agtacgaggt
     9421 tttctaccgt tggatgcgtt gcacgaagca gagattccgt acaaccggtg cactcctgcg
     9481 cgccaatcca gataactggc ggacgctgcg ggttagtaac cgattcggcc atctctgcag
     9541 cggctttgct acttaacccc atggtggcgg ctaatgctgc acaaagcttc atgaaatcac
     9601 gacggttaat gccgtgagaa tggatgaggg tgttatctcc agtcatttat agttattccg
     9661 ttgcgaagac ctggcatata ttttgcctca atcgcaaaat caataatgcg atcgatgcgc
     9721 catttaccac acatttatta tggttatcga tatcatgata ctgcggcggg gcgctaaaac
     9781 gaagggaaga agcattagtg taattaatta aagcaagata atacgtatgt ttgatcaatt
     9841 ttcgaggcga taaccggcca taaaaaagcg caacgtagaa caggaattat tctcacgaaa
     9901 gaaatccatt accatctctt tatccagtcc atagcggcgc gtcagaatac ccgcttccca
     9961 ggcgctaagc tggcgatctc cggtcttttc gtacatccgg tgtgagtagc ctaatacaaa
    10021 accgcgttta taatcggcac agaattttgt cacattaacc gcactgtcgg catgcgttgc
    10081 ttttaatccc gccattaagc ctttaccaaa atggttattc atcgttcacc tcattcgcta
    10141 tatatcgtaa tatatagcga tattttaggc aaagaacgac ttttgcgcac gctatttttt
    10201 catgcttgtt taagacgtaa tcccgtaaga gataaaccta tcaggagaga tatttatcga
    10261 accatttcag tatcctttgc caggcaagat cggcggcaga tttgtcataa cggggcgtgg
    10321 aatcattatg gaatccgtga ttaacccccg gatagatata cgcctcataa accttattat
    10381 tggctttcaa cgccgcctcg taagcaggcc agccctcgtt gattcgggtg tccagttccg
    10441 cgaagtggag tagtaaaggc gcttcaatct tcgccacatc ggcagtgggt gcctgacgac
    10501 cataaaacgg cactgcgcag gccagttccg ggtaagcgac cgctgccgcg ttcgatacgc
    10561 caccgccata gcaaaatccg gtaataccca ctttgcctgt cgcttgcgga tagcgttgca
    10621 taaactcaat tgcggcaaag aaatcattca tcagtttggt tggatcaacc tgctgttgca
    10681 gctcacgacc tttatcatca tttcccggat aacctccaac ggaatttaag ccgtcaggtg
    10741 ccagggcgat ataccccgcc ttcgccactc gccgtgccac atcttcgata tacggattca
    10801 gtccacgatt ctcatgcacc accaccacgg ctggcgtttt gccgctcatt tttgcgggtt
    10861 taaccagata accccgtacc tcgccgtgac catttggcga aggatacgtg atgtactcag
    10921 caaaaatctc cgggtcggta aactctacct gagtcgccag cgcataattt ggcttgagca
    10981 aatcaaacaa cgctaatgcc gtcatcccgc ctaccgcata cttcgccgca aggttgagga
    11041 actcacgttt cgagattttc ccgtgagcgt aatagtcgta gtaatccagc aactcttgtg
    11101 ggaaatcttt ggcggttaaa cgcggcatcg ttgcactcct cagttggtgt ttttttaagc
    11161 aaagcataag cacgtgtttt tgcccagttt ttcgtcactc tgtgagccag actacgggat
    11221 acgcgctggc gaatcgctaa actagaaaca ttgtttcgaa attgaacggt ggaaaggaga
    11281 ggtcatggtc tggttagcga atcccgaacg ttacgggcag atgcaatacc gctattgcgg
    11341 aaaaagcggt ttacgcctgc ccgcgttatc gctcggttta tggcacaatt tcggtcacgt
    11401 taacgcgctg gaatcacagc gtgcgatcct gcgtaaagcg tttgatttag gcattacgca
    11461 ctttgattta gccaacaatt acgggccgcc tccaggaagc gcagaagaga actttggtcg
    11521 cctgctgcgg gaggattttg ccgcttatcg cgatgaactg attatctcta ccaaagctgg
    11581 ctacgatatg tggcccggcc cttacggctc tggcggttca cgtaaatacc tgctcgccag
    11641 cctcgaccaa agcctgaagc gtatggggct tgagtatgtc gatatctttt actctcatcg
    11701 cgtcgatgaa aatacgccga tggaagaaac cgcctctgcg ctggctcatg cggtacaaag
    11761 cggtaaggcg ctgtatgtcg ggatctcctc ttactcacca gagcggacgc aaaaaatggt
    11821 cgagttgctg cacgagtgga aaattccgct gttaattcat caaccttcgt acaatttact
    11881 aaaccgctgg gtggataaaa gcggcctgct ggataccctg caaaataacg gcgtgggctg
    11941 cattgccttt actcctctgg ctcagggatt gctgaccgga aaatatctca acggcattcc
    12001 tgaagattca cggatgcatc gtgaagggaa taaagttcgt ggtctgacgc cgaaaatgct
    12061 caccgaagcc aacctcaaca gcctacgctt attgaatgaa atggcacagc agcgtggaca
    12121 atcaatggcg caaatggcgt taagctggtt gctgaaagat gagcgagtga cgtcggtatt
    12181 ggttggtgcc agccgcgcgg agcaacttga ggagaacgtg caggcgctga ataatctgac
    12241 atttagcacc gaggagctgg cgcagatcga tcagcatatc gccgatggcg agctgaatct
    12301 gtggcaggcg tcttccgata aatgacctgt taataacggg cgcgagaacc gcgcccgaat
    12361 cagatcagtg attatgacga gtcagtcggt caagatagcc cattacaaat gcagagagca
    12421 caaacgtcag atggataatg acgtaccaca tcagtttgtt atcagggaca tttttcgcat
    12481 ccataaagac gcgcagtaag tgaatggaag aaattgccac aatcgacgct gctactttgt
    12541 ttttcagcga cgttgcatcc attttcccca gccagttcag cttctctttg ttctcggaga
    12601 tatcgagttg tgagacgaaa ttctcataac cggaaaacat caccatcacc agcaaaccgc
    12661 caaccagcgt catatccacc agcgacagca acacgaggat caaatctgat tccgccatcg
    12721 agaagatatt cggcagtacg tgaataatct cctggaagaa cttcagcgcc agggcaacta
    12781 acgccagcga aaggccaaag tacacggggg caagcagcca gcgagaagca tacattgcat
    12841 tttcaagaaa acgttccata cattcctgtc gtgtagttaa aacgggcaac agtatatcgc
    12901 aattgagtaa acagcgcgca acagcaaatc aacatatccg ttaaatcttt gtcgcataat
    12961 tctgagggtt agggatcttc ctggttccct tatcaacgca tcgagagcgt ctacccttaa
    13021 aggacgttga acctgaaggg agaaaaacga tgtctcattt aaaagacccg accacgcagt
    13081 attacactgg tgaatatccc aaacagaaac aaccgacgcc aggcatccag gcgaagatga
    13141 caccggtacc ggattgcggc gagaaaacct atgttggtag cggtcgcctg aaagatcgta
    13201 aagccctggt gacagggggc gattccggaa tcggtcgcgc tgccgccatc gcttacgcgc
    13261 gtgaaggggc tgacgtggcg atcagttatc ttcccgtgga agaagaagac gctcaggatg
    13321 tgaaaaagat cattgaagaa tgcggacgca aagccgttct gctgccaggc gatttaagcg
    13381 atgagaaatt tgcccgttcg ctggttcacg aagcgcataa ggccttaggt gggctggata
    13441 ttatggcgct ggtcgccggg aaacaggttg ccattcctga tattgcagac ctcaccagcg
    13501 aacagtttca aaagaccttt gccattaacg ttttcgcgct gttctggcta acccaggaag
    13561 cgatccccct gctaccgaaa ggtgcaagta ttatcaccac ttcgtcaatc caggcatacc
    13621 agccaagccc gcatttactg gactatgcag ctacgaaggc ggcgattctg aactacagcc
    13681 gaggcctggc aaaacaggtc gcggagaaag gtattcgggt gaatattgtc gcgccaggcc
    13741 cgatctggac agcactgcaa atttccggcg gacaaacgca ggataagatc ccgcagtttg
    13801 gtcagcaaac gccgatgaaa cgtgcggggc aaccggcgga actggcccct gtatatgttt
    13861 atctggcaag tcaggagtcg agctacgtca ccgcagaagt gcacggcgtg tgcggcggcg
    13921 agcatttagg ttaaaaaaat gcccggttgt gaaaagcaac cgggcatcat tgtgaattca
    13981 ctcttacctg atgtggcgaa aacgccttat ccgcctacaa atgcatgaag attcaacatg
    14041 ttgcaataga ttttgtaggc ctgataagcg tagcgcatca ggcattgagc acctaatgcc
    14101 ggatgcggcg tgaacgcctt atccggccta caaacgcacg aagattcaac atgttgcaat
    14161 agattttgta ggcctgatga acgtagcgca tcaggcaatt ctactttact tcgctttggc
    14221 ggtttcttcg ccgaccagac ctatcttcag gtaacccgcc tgatgcagcg tgtccattac
    14281 cttcatcaac gtctcgtaat cgacagtttt atccgctcgg aagaagatgg tggtgtcttt
    14341 cttgccttcg gttaacgcat tcaacgccgt aatcattgtt tcatcggtga ccggatcgtt
    14401 accgataaac atcgagttgt ctgccttcac cgacagataa accggttttt ccgggcgcgg
    14461 ctgcggcgtg ctggtagaag caggcaagtt aaccttcaca tctaccgtcg ctaacggtgc
    14521 cgccaccata aagataatca gcaggaccaa catcacgtcg ataaacggcg tcacgttgat
    14581 atcatgcatt tcgccgttat cgtcgaggtt ttcgttaaga tgcattgcca tcggacatta
    14641 tcctgcgcgt aatttttgtg cgacacgaac cggatgcgca gcggcgctgg cttccagatc
    14701 caggtcacgg ctttgcagca acaatacctg cgctgcgaca tcacccagca tcgctttaaa
    14761 gccgccaatc tgacgcgcga atacgttata gataacgacc gccggaatcg ctgccaccag
    14821 accgattgcc gttgctaaca gagcttctgc gatacccggc gcaacgactg ccaggttagt
    14881 ggtctgcgtt tgcgcgatac caataaagct gttcatgatg ccccagaccg taccaaacag
    14941 cccaacgaac ggcgaaatcg cgccgatggt tgccagatag ccgttaccgc ggcccatttg
    15001 acgacctact gcggcgaccc gacgctccag acggaagctg gtacgttctt taataccttc
    15061 gttatcgtcg ctgccttctg acagttccag ctcattctgc gcttcattga gcaaatgcag
    15121 gcttaagctt ttgctaccaa aatcagcggc gatatcgttg gcctggttta aggaacgcgc
    15181 ttcagccagc agttgctgct cgcgcttaag gcgacgcttc tgattaaaga actctacgct
    15241 cttactgaag aagattgccc aggtgactac ggaggccaaa ataagcccaa tcatcacgca
    15301 cttaacgaca atatcggcgt gctgatacat accccagacg gaaaggtccg tctgcattaa
    15361 attattaccc actgtgtatc tccaggacgc aagtcacaaa atctgcgcat aataatatca
    15421 aaacgacgtc gaattgatag tcgttctcat tactatttgc atactgccgt acctttgctt
    15481 tcttttcctt gcgtttacgc agtaaaaaag tcaccagcac gccatttgcg aaaattttct
    15541 gctttatgcc aattcttcag gatgcgcccg cgaatattca tgctagttta gacatccaga
    15601 cgtataaaaa caggaatccc gacatggcgg acaaaaagct tgatactcaa ctggtgaatg
    15661 caggacgcag caaaaaatac actctcggcg cggtaaatag cgtgattcag cgcgcttctt
    15721 cgctggtatt tgagagtatg gaagccaaaa agcacgcgac gcgcaatcgc gccaatggcg
    15781 agttgttcta tggacggcgc ggaacgttaa cccatttttc cttacaacaa gcgatgtgtg
    15841 aactggaagg tggcgcaggc tgcgcgctat ttccctgcgg ggcggcagcg gttgctaatt
    15901 cgattcttgc ttttgtcgaa cagggcgatc atgtgttgat gaccaacacc gcctatgaac
    15961 cgagtcagga tttctgtagc aaaatcctca gcaaactggg cgtaacgaca tcgtggtttg
    16021 atccgctgat tggtgccgat atcgttaagc atctgcagcc aaacactaaa atcgtgtttc
    16081 tggaatcgcc gggttccatc actatggaag tccacgacgt tccggcgatt gttgccgccg
    16141 tgcgcagtgt ggtgccggat gccatcatta tgatcgacaa cacctgggca gccggtgtgc
    16201 tgtttaaggc gctggatttt ggcatcgatg tttctattca agccgccacc aaatatctgg
    16261 ttgggcattc agatgcgatg attggcactg ccgtgtgcaa tgcccgttgc tgggagcagc
    16321 tacgggaaaa tgcctatctg atgggccaga tggtcgatgc cgataccgcc tatataacca
    16381 gccgtggcct gcgcacatta ggtgtgcgtt tgcgtcaaca tcatgaaagc agtctgaaag
    16441 tggccgaatg gctggcagaa catccgcaag ttgcgcgagt taaccaccct gccctgcctg
    16501 gcagtaaagg acacgaattc tggaaacgag actttacagg cagcagcggg ctattttcct
    16561 ttgtgcttaa gaaaaaactc aatgatgaag agctggcgaa ctatctggat aacttcagtt
    16621 tattcagcat ggcctactcg tggggcgggt atgaatcgtt gatcctggca aatcaaccag
    16681 aacatatcgc agccattcgc ccacaaggcg agatcgattt tagcgggacc ttgattcgcc
    16741 tgcatattgg tctggaagat gtcgacgatc tgattgccga tctggacgcc ggttttgcgc
    16801 ggattgtata acattgccac ttttggacaa ttttgcagac attttattgt gaaaagtctt
    16861 aaattgttgc gtccgggatc aaggcgtccc ggacgattca ggagtacaat aggcagataa
    16921 aggcttaaac gctgttccac aggaaagtcc atggctgtta ttcaagatat catcgctgcg
    16981 ctctggcaac acgactttgc cgcgctggcg gatcctcata ttgttagcgt tgtttacttt
    17041 gtcatgtttg ccacgctgtt tttagaaaac ggcctgctgc ccgcctcatt tttgccaggc
    17101 gacagcttgt tgatattggc tggcgcattg attgcccagg gggttatgga ttttctgcct
    17161 acgattgcga ttctgaccgc cgcagcaagt ctgggctgct ggctaagtta tattcagggg
    17221 cgctggttag ggaataccaa aacggtgaaa ggctggctgg cacagcttcc tgctaaatat
    17281 caccagcgcg ccacctgcat gtttgaccgc cacggtctgc tggcgctgct ggctggacgt
    17341 tttcttgcat ttgtccgtac gctgctgcca accatggcgg gaatttccgg tctgccaaac
    17401 cgccgtttcc agtttttcaa ctggttaagc ggattgctgt gggtcagcgt ggtaaccagt
    17461 ttcggctatg ccttaagtat gattccgttc gttaaacgcc atgaagatca ggtaatgacg
    17521 ttcctgatga tcctgccaat tgccttgtta accgctggct tgttaggcac gctgtttgtg
    17581 gtgattaaaa aaaaatactg taacgcctga cgattttccc tgttcccggt tgctgtaccg
    17641 ggaacgtatt taattcccct gcatcgcccg cattcttgcc gcatcttccc ccggcgtcac
    17701 accgaagtaa cgtttaaact cacggctaaa ttgcgatgcg ctttcatagc cgacgcgcat
    17761 cgctgctgcg ctggccttca tgccgtcatg gatgatcatc atccgcgcct tatgcagacg
    17821 gtaattcttc aaatactgca acggcgaggt gctggtgaca gacttaaaat tatggtggaa
    17881 cgccgatacg ctcatattag cttctgccgc cagttgctcg acgctcaggt tttcggtgta
    17941 tttattctca atccgtttca gcacgcggct aatcagactg aagtgagtct ggcgactgac
    18001 cagcgccagt aacgcgccgc cgcaaggtcc ggtcagcacg tagtacagaa tttcgcggat
    18061 gatctgtttg ccgagaatac gcgcatccag tggtcgcgcc atcacgtcga gtaaccgctc
    18121 cgccgcgcat aaaatctctt ctgataacgt ggcggagtta atcccgctgg ctgccatcga
    18181 cggctggaaa tgctcatctt cgccaatatc catcaacagt tcctgtaact gcaaaatatc
    18241 gacattgaga cgcaaccctg ccagcggcac ctctgacgtg gcataggttt cgcactcaaa
    18301 cggcaacggc accgtcagca gcaggtattc attggcatca taacgaaaca cgcgttcatt
    18361 gatataaccg attttatgtc cggaaaagag aattatgatg ccaggctcgt acatcaccgg
    18421 tgtacgtgcg aaaggcgtct cgccatacaa caaacgcaca tcgggcaaca gttctgacaa
    18481 actattttct ttatttttca gtttattaac tttatccgcc agcaagcggc aaatctcttc
    18541 acgtttcata tcgcgtaatt tcttaggaat aatgcggcaa tttgattgtg cgcaattttg
    18601 tagcatttct ccagcactct ggagaaatag gcaagacatt ggcagaaatg agcattgaga
    18661 gccagggcgc tggcgatcac aatgaaaaac atcaggcaga tcgttctctg ccctcatatt
    18721 ggcccagcaa agggagcaag taatgaacaa ctttaatctg cacaccccaa cccgcattct
    18781 gtttggtaaa ggcgcaatcg ctggtttacg cgaacaaatt cctcacgatg ctcgcgtatt
    18841 gattacctac ggtggcggca gcgtgaaaaa aaccggcgtt ctcgatcaag ttctggatgc
    18901 cctgaaaggc atggacgtgc tggaatttgg cggtattgag ccaaacccgg cttatgaaac
    18961 gctgatgaac gccgtgaaac tggttcgcga acagaaagtg actttcctgc tgggggttgg
    19021 cggcggttct gtactggacg gcaccaaatt tatcgccgca gcggctaact atccggaaaa
    19081 tatcgatccg tggcacattc tgcaaacggg cggtaaagag attaaaagcg ccatcccgat
    19141 gggctgtgtg ctgacgctgc cagcaaccgg ttcagaatcc aacgccggtg cggtgatctc
    19201 ccgtaaaacc acaggcgata agcaggcgtt ccattcagcc catgttcagc cggtatttgc
    19261 cgtgctcgat ccggtttata cctacaccct gccgccgcgt caggtggcta acggcgtagt
    19321 ggacgccttt gtacacaccg tggaacagta tgttaccaaa ccggttgatg ccaaaattca
    19381 ggaccgtttc gcagaaggca ttttgctgac gctgatcgaa gatggtccga aagccctgaa
    19441 agagccagaa aactacgatg tgcgcgccaa cgtcatgtgg gcggcgacgc aggcgctgaa
    19501 cggtttgatt ggcgctggcg taccgcagga ctgggcaacg catatgctgg gccacgaact
    19561 gactgcgatg cacggtctgg atcacgcgca aacactggct atcgtcctgc ctgcactgtg
    19621 gaatgaaaaa cgcgatacca agcgcgctaa gctgctgcaa tatgctgaac gcgtctggaa
    19681 catcactgaa ggttccgacg atgagcgtat tgacgccgcg attgccgcaa cccgcaattt
    19741 ctttgagcaa ttaggcgtgc ctacccacct ctccgactac ggtctggacg gcagctccat
    19801 cccggctttg ctgaaaaaac tggaagagca cggcatgacc caactgggcg aaaatcatga
    19861 cattacgctg gatgtcagcc gccgtatata cgaagccgcc cgctaagctt tttacgcctc
    19921 aaactttcgt tttcgggcat ttcgtccaga cttaagttca caacacctca ccggagcctg
    19981 ctccggtgag ttcatataaa ggaggaacgt atggctaatc caaccgttat taagctacag
    20041 gatggcaatg tcatgcccca gttgggactg ggcgtctggc aagcaagtaa tgaggaagta
    20101 atcaccgcca ttcaaaaagc gttagaagtg ggttatcgct cgtttgatac cgccgcggcc
    20161 tataagaatg aagaaggtgt cggcaaagcc ctgaaaaatg cctcagtcaa cagagaagaa
    20221 ctgttcatca ccactaagct gtggaacgac gaccacaatc gcccccgcga agccctgctc
    20281 gacagcctga aaaaactcca gcttgattat atcgacctct acttaatgca ctggcccgtt
    20341 cccgctatcg accattatgt cgaagcatgg aaaggcatga tcgaattgca aaaagaggga
    20401 ttaatcaaaa gcatcggcgt gtgcaacttc cagatccatc acctgcaacg cctgattgat
    20461 gaaactggcg tgacgcctgt gataaaccag atcgaacttc atccgctgat gcaacaacgc
    20521 cagctacacg cctggaacgc gacacacaaa atccagaccg aatcctggag cccattagcg
    20581 caaggaggga aaggcgtttt cgatcagaaa gtcattcgcg atctggcaga taaatacggc
    20641 aaaaccccgg cgcagattgt tatccgctgg catctggata gcggcctggt ggtgatcccg
    20701 aaatcggtca caccttcacg tattgccgaa aactttgatg tctgggattt ccgtctcgac
    20761 aaagacgaac tcggcgaaat tgcaaaactc gatcagggca agcgtctcgg tcccgatcct
    20821 gaccagttcg gcggctaacg tgcaaattct cccggtggcg gtaatgttcc gctaccggac
    20881 ttttcagaaa tcatttattc ccctcgcgtc ccgcccgttg ttactcttcc ttgttcagga
    20941 atgccaaata taaggacatc atcatgcaga gccggaagct cttaaaagaa caactcatct
    21001 atatccggga taaacgcaac ggagaggtga aaaacagatg aaaataatac ttctgttttt
    21061 agcagccctg gcaagtttta ccgtgcacgc acagcccccc tcacagaccg tagaacaaac
    21121 agtccggcat atttatcaga actataaatc agatgccact gccccttatt ttggtgaaac
    21181 cggagagcgt gcgataactt ctgtgcgtat tcaacaggcg cttaccctga acgacaatct
    21241 tacgctgccg ggtaatattg gctggattat gatccggttt gtgattgtca ggattttggc
    21301 gatctggtgc tagaaagcgt agcgataacc gaaactgacg ccgatcatgc cgatgccgtt
    21361 gtgcgctttc gtatctttaa agatgataaa gaaaagacca cgcagacact gaaaatggtg
    21421 gcggaaaatg gtcgttgggt cattgacgat attgtcagca atcatggcag cgtcttacaa
    21481 gcagttaata gcgagaatga aaaaacgctg gccgctttag cttcgttgca aaaagaacag
    21541 ccggaagcct ttgttgccga actctttgaa catattgccg attatagctg gccgtggacg
    21601 tgggtggttt ccgactctta ccgccaggcg gtcaatgcct tctataaaac caccttcaag
    21661 acggccaaca atcccgatga agatatgcaa atagaacggc aatttattta cgacaatccg
    21721 atctgttttg gcgaagagtc gctattttca cgcgttgatg aaattcgagt cctggagaaa
    21781 accgccgatt ccgcccgcat tcatattcgt tttacgctga ccaatggcaa caacgaagag
    21841 caagaactgg ttttacaacg gcgcgaaggt aagtgggaaa tcgctgattt tatccgcccg
    21901 aacagcggca gcctacttaa gcagattgag gcaaaaaccg ccgccagatt aaagcaatga
    21961 gctgaattaa atagcaatta gccggaacaa taaaaaaggg aacactatat gaaaacgatt
    22021 ttcaccgtgg gagctgttgt tctggcaacc tgcgtgctca gtggctgcgt caatgagcaa
    22081 aaggtcaatc agctggcgag caatgtgcaa acattaaatg ccaaaatcgc ccggcttgag
    22141 caggatatga aagcactacg cccacaaatc tatgctgcca aatccgaagc taacagagcc
    22201 aatacgcgtc ttgatgcaca ggactatttt gattgcctac gctgcttgcg tatgtacgca
    22261 gaatgataaa aaaatccccg gcagcatgtc agttgccggg gatttttttt aacgtccaac
    22321 cgccgcttta gggcgtttct tcgcaccagc attcaccgga cgagattgcg tagacgacgc
    22381 tttttttgcc gtagcaggcg tctgacactg ggtcgccatc ggcgtatgtt tcgtcaacgc
    22441 cggacgggta ttgcggttct ggcgacgagc ctcacgcatc tcttcaatgg ttggcgcagg
    22501 cactaagcaa tcgcgacggc tgccaatcag atgctttttg cccatcgctt ccagcgcctg
    22561 gcggattaac ggccagtttg ccggatcgtg gtaacgcaac aacgctttat gcaaacgacg
    22621 ctgtttgtcg cccttcggta cgaagacgtc ttcactctta taaccaatct tcgccagcgg
    22681 gtttttcccg gtgtaataca tggtggtcga gttagccagt ggcgatgggt agaagttctg
    22741 tacctggtcg agacggaaac gatgcttttt cagccacagc gccaaattca ccatatcttc
    22801 atcacgcgta ccggggtgcg cggagatgaa ataggggatc agatactgct ctttacctgc
    22861 ctgtttcgag taggtatcga acagctcttt aaagcggtca taactgccca tgcccggctt
    22921 catcatcttc gacaacggcc cttcttcggt atgttccgga gcaatcttca gataaccgcc
    22981 gacgtgatgg gtcgccagtt ctttgatata gcgcggatct tccacggcga tgtcataacg
    23041 tacgcctgag gcaatcagaa tctttttaat gcctttcaga tcgcgagcgc ggcgatagag
    23101 gttgatcgtc ggttcgtggt tagtgtccat atgcggacaa atatccgggt aaacgcagga
    23161 caaacgacgg caggtttgtt cagcgcgtgg cgatttgcag cgcaacatat acatgttggc
    23221 agttggccca ccgagatcgg aaattacgcc cgtaaaacct ggaacggtgt cgcggatcgc
    23281 ttcgatctca ttaatgatcg aatcttcgga acggctctga ataatgcgcc cttcgtgctc
    23341 ggtgatagaa cagaaagagc agccgccaaa gcagccgcgc ataatgttga ccgaaaaacg
    23401 gatcatttcg taagccggaa tacgggcatt gccataggcc ggatgtggca cgcgcttgta
    23461 cggcagcgca aaaacgctgt ccatctcttc ggtagaaagc gggatagcag gcgggttgat
    23521 ccacacatag cggtcaccgt gtttttgcat caatgcgcgg gcgcaacctg ggttagtttc
    23581 gtggtgcaga atacgcgaag catgggcgta cagcacttta tcgcccttca ctttctcgaa
    23641 agaaggcagc aacacgtagg ttttttccca cggtttcggg cgcggtggct gcacggttac
    23701 ggctttggct tcctgctttt tcggtgccac cggtttgtta tccgcgcacg gcaaatcttc
    23761 accatacgga tgcgggattg ggtcgatttt tccaggggta tcaagacggg tggaatccac
    23821 gccgctccag ccaggcagcg cctctttcac gataatcgcg gtattacgca catcgcggat
    23881 ttcactaatc ggctcgccca tcgccagacg gtgcgccacc tccaccagcg gacgctcacc
    23941 gttaccaaac atcagcatgt cggctttcga atccaccagc acggaacggc gcacggtatc
    24001 ggaccagtaa tcataatgcg cggtacggcg cagactagcc tcaataccac cgaggatcac
    24061 cggtacatct ttccacgcct ctttacaacg ctgggtataa accagtgtgg cgcgatccgg
    24121 gcgcttaccc gcgacgttat ccggcgtgta ggcatcgtca tgacgtaaac ggcgatcggc
    24181 ggtataacgg ttgatcatcg aatccatgtt gccagcagta acaccgaaaa acagattcgg
    24241 tttacccaga cgcatgaaat cgtctttgct gctccagtct ggctgggcga tgatcccgac
    24301 gcgaaagccc tgtgcttcca gcatacgacc gcaaatcgcc atcccgaagc ttgggtgatc
    24361 gacatacgcg tcgccagtaa ccaaaatgat gtcgcagcta tcccagccaa gttgatccat
    24421 ctcttcacga gacatcggca aaaacggtgc cggtccaaaa caggcggccc agtactgcgg
    24481 ccaggagaac aggtcgcgat ccggttggat cagggagata gagctcattt tgcttccaga
    24541 aatgataaaa aaataatcaa aggccgggga ttataagccg gaacgaaaga gaaatcgaaa
    24601 agtattccat actcgccctc ctcgggcgag tatgaagatt acggtaccgg attgactaac
    24661 agttgcccaa tcgagccacg gtccgccatt tccagcgtct gactgttgaa gtagaacggg
    24721 aagtgcgccc aggaaggctg accgaaataa acaagcagct ccacttgtcc gtcaacccaa
    24781 acggtatctt tccagcctct gtcttccggg aacggcatcg caccattcac gttacggatc
    24841 tggaacatca cgccttcaat atggaacgct tgcggctcgt ccgcgcgtac cgtccagcgt
    24901 tcccacgttc cttgctgcgc ggtgacatca atacggttga cgtcccacag ttgtccatta
    24961 atacccgggt catcacccag actgatatcg cgactgcgaa tcggcgaacc ggccatgatt
    25021 tcagttggca gcaagcgcat cggaagactg tctgtgacca gcggcagaag gccggttggg
    25081 cgcagcgtta gcaccagggt agaaaccaga atgctggatg gctcaaagaa gccacgaata
    25141 cgatcaacaa tgctcgccgc ttcgccacag gtgatcgaca cttcatcacc gttgctcata
    25201 tccaccagaa tctcgcggcg ctcgcccggt gccaacgaaa gttgcttcac cgatacagga
    25261 gcaggcagga atccctgatc gccagaaatc acatgtaacg ggcgaccatc gctcatctgt
    25321 agttgatagc gacgagagtt cgacgcgttc agcagtcgca agcgcaccca gccacgcgag
    25381 acttcaacgt acgggctttg tacaccgtta accagcagcg tatcaccaac aaagccgccg
    25441 cttcccggtt cgttgtattc tggcgtacca aagttatcca gccgtttatc ctggataatg
    25501 accggaaaat catccacacc ataatggttg gggataggca gcgacttgct gacttcatct
    25561 tccaccagcc acattccggc aaggccgtta tagacctgct gagccgtgcg gttgggagta
    25621 ttggcgtgat accacagagt agctgcgttc tggcgaatag gcagtacggg tgcccagtca
    25681 gcgtttggcg acatcatccg tgccggaccg cccatcagcg ggcctggtac ctgtagcccg
    25741 gccaccgtca ttgagacatt ttctgtcagg cggttgctgt aaataagctt aacatcgtcg
    25801 cccttccaga cgcggatagt cggccccagg taacgaccat tgattcccca gaccgacgcg
    25861 cgtgtccctg gcgtaaatga ccagtgcgca cgttgtacag tcataaacag cggttgccca
    25921 cggcgagatt caagcagcgg cggaacgggt agcggttgct gttgcccggc tgcgctggcc
    25981 ttcaggggaa cagcgcctgc acaaagtgca atccccgatg cctgaatgaa ctgacgccga
    26041 ctgagtgaca tatttgctcc atgtaaaact ggctaaagta ataccgtgca ggaaagtgtt
    26101 tcccgcaaaa atacagattg ccgacttaaa cttttccggc ggcttcgcgc tctgcgactt
    26161 ctttatcgag ctcggcgatt ttttgttcca ttatcgaacg acaatgggca gccagctcac
    26221 gaacctgatc tttgccatac tgactgacgt caattggcgg cagcatttcg acaatcacca
    26281 gaccgttgtg cagtcgatta agattaatct tattcgaagt tgtggagacg cacacgggaa
    26341 taatcgggac gcccgccgca attgccgcgt gaaatgcccc agtcttgaac ggtagcaggc
    26401 cgcgaccacg gctgcgggta ccttccggga acatccagat ggaaatacgg cgttttttga
    26461 aatgattcac tacttccgca atagtgccgt gagctttagt gcgattgttt ctgtcgatca
    26521 ataagttgcc ggttaaccag tacaactgcc cgaagaaggg gatccacagc aagctctttt
    26581 tacctaccgt caccgtcggc ggttgcacaa tgttcgatgc tgtcaccatg tcatagttgt
    26641 tctggtggtt agcgatatag atagcattgc cgtagctttc agcatctgca ggtttgcggc
    26701 actcaacttt caggccgaac agcggcgcaa gacgaccaaa catatgccca aaggtggcca
    26761 catgtttcgg gttacgcggg ctgaaaaggc agtaaatgga gccgaataca cagactaaga
    26821 tgctgtaaat cacggtaata ataagacgaa agatatatag cataacaccc tctgaaggtc
    26881 tgagagactg gcattgtacg tcacctgaaa tcaggttagg gcattgttaa aagccgctct
    26941 ctggaaaagc gtattgttaa tcgcaacgca tgaataaaca acggtttcaa gggaattttt
    27001 atcgtttccg ggggttgaat acgctgccgg atgacgactt aacgtttcat ccggcgttcc
    27061 ttgcaagcgg gaggaaacag cgccctcccc ggcatattac tcttcgctat caccgctgct
    27121 ggcacggcga ggagagtcga tctcaacacg atcgatacgc tgcaaaccgc gcatcaacgt
    27181 accgcggcgt ccacgttcgc cagtgacttt ctgtagctct tccggacgca gtttaatttt
    27241 gcgtttccca acatgaatgg tcagcgtgct ttgcggcggc agaacgtaca gttgcgccag
    27301 accatcctcg ccacgcgcgg cttctgccga tggaatgttg ataatcttgt tgcctttgcc
    27361 cttcgacagc tgcggcagat cacttaccgg gaacatcaac atacggcctg cctgagtgat
    27421 tgccagcagc atatcggaag catcttcaat taccaccggc ggcataacat gggcattttc
    27481 cggtaaggtg atcaaagcct tacctgcacg gttacgcgcc accagatcgt taaaggtgca
    27541 gacgaaaccg taacccgcat cggaagccat cagcagtttc tgatcgtcgc tttccatcag
    27601 catatggtca acggtcgccc caggcggcaa cgttaatttg ccggtgagcg gttcgccctg
    27661 accacgcgcc gacggcagcg taatcggatc aatggcatag ctacgaccgg tggaatcaac
    27721 aaacactacc ggttggttgc tcttaccttt caccgccgct ttgaagctat cacccgcttt
    27781 ataattcagg cccggcgcgt cgatatcatg gcctttagcg ctgcgtaccc agcccatctg
    27841 cgacagcaca atggtgacag gttcagacgg cagcatgtcg tgctcgctca tcgctttcgc
    27901 ttcttcgcgt tcctgcaacg gcgaacgacg atcgtcaccg taggcttgcg cgtctgcctg
    27961 cagttctttc ttcagcaggt tattcatttt acgctcggaa gccaaaatgc cctgcaactg
    28021 gtcgcgctct ttttccagct cactctgctc accgcgaatc ttcatctctt ccagtttggc
    28081 aagatgacgc agtttcagtt cgaggatcgc ttccgcctgg gtttccgtaa ggccaaaccg
    28141 cgacatcagc gccggtttcg gttcatcttc attacgaatg atctcaatca cttcgtcgat
    28201 attgagaaac gccaccagca aaccttcgag gatatgcagg cgcttgagga ctttctccag
    28261 acgatagttc agtcggcggc gcacggtatc gcggcggaac accagccatt cggagagaat
    28321 ttccagcagg tttttcaccg ccggacgacc atccagaccg atcatattaa ggttaatgcg
    28381 atagctcttt tccagatcgg tggtagcgaa gaggtggttc atcacctgat ccatatccac
    28441 gcggttggaa cgcggcacaa tcaccagacg ggtcgggttc tcgtggtcag attcatcgcg
    28501 cagatcgtca accatcggca gctttttgtt gcgcatttgc gcagcaattt gctccagtac
    28561 gcgcgcacct gaaacctgat gcggcaatgc gctgataacc accgcgccat cttctttctt
    28621 ccacaccgcg cgcatacgca ctgaaccacg tccgttctcg tagattttac ggatctcggc
    28681 gcgcgaagtg ataatttccg cttcagtcgg ataatccggc ccctgcacga tatccagcag
    28741 ctgatcgagc gtggttttcg gctggtcgat taatgcgatt gccgcctgag ccacttcacg
    28801 caggttatgc ggtggaatat cggtcgccat gccgacggca ataccggtgg tgccgttaag
    28861 caaaatgttt ggcagacggg caggtagcat tttcggctcc tgcaaagtgc cgtcgaagtt
    28921 tggcacccag tcagccgtcc cctgccccaa ctcgctcaat agcagctcgg aatatttcga
    28981 caaccgggat tcggtgtaac gcattgccgc gaacgatttc ggatcgtccg gcgcgcccca
    29041 gttcccctga ccatcaacca gcggataacg gtaagagaac ggctgcgcca tcaggaccat
    29101 cgcttcataa caggcgctat cgccgtgcgg atggtattta cccagtacgt caccgacggt
    29161 acgggccgat tttttaaatt tggcgctggc attcaggccc agttcagaca tcgcatacac
    29221 aatgcggcgc tgaacaggtt tcagaccatc accaataaac ggcaacgcac ggtccatgat
    29281 cacgtacatg gagtagttta agtaggcgtt ttccgtaaat tcatgtagcg caaggcgctc
    29341 tgccatatcg ctcattaatt ctgattcctc aacttattcg ccagccttca aaggggcaat
    29401 attgccggag atactacatt atctgccgcg tcgagtcaca ggagatcagc gtgaatagcg
    29461 ccgccggacg tgctccgacg gcgataaagg catcaatgtg ccttgatata caactctttg
    29521 ctgtagacat aatcctgggg attatgcagc ggaaaaccgc caacgtaggg tttaagcagt
    29581 ttgattaacg gctgatagta gataggaatc agcggtgcct gctggttgac gatcacttct
    29641 gcctgctgat acaacgcatt acgctttgtc gcatcagtga tctgcatggc ctggtttagt
    29701 aaggcgtcat actgcgcatt tttccagtga ccgacgtttt cttcactatc gcttttgagc
    29761 gtgttcagga agctggaagc atcattgtac gtcgcatccc acgactgccg agacagcatg
    29821 aaatcaccgg ctcgtcgggc atcaagatag gttttccact ccattgtgcg cagcgtcacc
    29881 tgtgcaccca gccatttttt ccattcggaa gacaacgcta tcgcggtctt ttcatgcaga
    29941 tcgtacttgt tgtagaacag ctcaaagcgt agcggatgag aggcgtcgta tcccgcctgt
    30001 ttcagcaagg cttttgccat cgcgacgcgc tcactcattg gcttttgcag ttcatcgaac
    30061 gtcgtcgcgc taaagccttt tacctctggc ggcgtcagcg tggttgcggg cgttctcaac
    30121 cccagtacct tttgcgcaat aagctgtcga tcaaccgtaa gatatagcgc ccgacgcact
    30181 cgcacatcgt taaatggcgg tttctcaagg ttgaagttgt aatattcgct gttcagacgc
    30241 ggaataattc gtatctcgcc aggcagtgat ttttcaatgg cgggaatttg ctgcgccgga
    30301 acccaggtga gatcgacctc tcccgcgcga tagcggttat agccggtgac cgaattatcc
    30361 agcgccagat actcaacctg ttgcaatact gtatgttgcg catcgcggta ctttggattt
    30421 ttgcgtgcag taatcttttc gttaactacc cactgatcaa gcacaaaggc accgttgtaa
    30481 accatgttct ctggcttact ccagctatcg ccatgtttag cgatgacatg atgaggaacc
    30541 gggaacagcg tcggccaggc gagcatcgtc gtgaaccaag gaaccggctg ctcaagcgta
    30601 acttcaagag tacgatcatc cgtcgctttg acacccagcg atgtaacatc tgctttaccc
    30661 gcaacaatag ccgcggcatt gttaatgtgc gcctgtgcca gatagccagc aaaagggctt
    30721 gccgttttcg ggtcaaccgc gcgctgccag ccgaggacaa aatcctctgc cgtcagaggc
    30781 tgaccgtcgg accactgcaa accgctacgc agatggaaaa tatagcgcct gccgccgtcc
    30841 agtatctccc agcgttcagc ctgagcgggc tgcacctggc cttcaccgtc catccatacc
    30901 agaccttcaa acagatccag cacaatctgc gcggcagtat tctcctccac cttttgcggg
    30961 tcgagcgtac ctgggtcgct atgattgttg taacgaaaga cttgttgcgg ggcgagctgt
    31021 gtgttggtgg gaacgtcagc agcgtagaga ggagccgcac ttaccaggct gaccagccat
    31081 aataaatttc gcgtatacat ggtattcctt accggagaaa ttataagccc aacaacaata
    31141 cgcctgtggc attgttcgct caaacttatc gcgagtgatt tggctcacac tccggtaaag
    31201 attccccatc acgctaacgg cacataaaca ttcgtttcca gtcgctgctc cgtcacccct
    31261 tcggcaagat tggtgtagtg aaacagaatc ggtgctttac gcattttctc gccgcttgca
    31321 ggcagccagt ggcgaataat gccccatatc gtgtgtgaaa tatcgtccag ctcgccaacg
    31381 tggcgagcca cggcataacg tccaccggta agttcaccat tgctcacacc ataacgatta
    31441 tcgggaatcg gttcgctaac gctgccgcag atatcaaagc gaaacgcttc cggtgcggtg
    31501 gtggcagggt cgtcccaggc gacgccaaaa gtctggcttt ggttaacagg agaaagcccc
    31561 gtctctttac gccacatgat aaatttcgcc acgctgtagt tgagcaactc aggcctacag
    31621 cggtgcggca acatcgccac agggattgat gggaaatcga taatgtttac atccagtgtc
    31681 aggtttgtca tgttgcctcc ctgcgaaatt attcccggga acgcatcccg ggagcggtaa
    31741 caattacgga tttactttgc ggatctgttt gacgtcaatt tcaacagaat tccagtcttt
    31801 atcgacttca ccctgaatct caaccgtatc tttcggcgtc accgtcacgc cgttccagcg
    31861 tttgtggtcg atatcaacat tgatagtacc gctggcatct ttgaacacgt agagatcgtc
    31921 agagatgcgt tcaacgatat tgccgcgcag ggttacccag gtgtcgtcac gcagggattt
    31981 tgcgctttct acagtcgtta cgctgccgtt cggcccctgg aatcctccgg cctgactttg
    32041 cgttgccgat gggccagaaa aaccgccctg ctctgctgcc atcaccggtg cgctgcacag
    32101 ggccattact gcgattactg ctgcgaattt tttcatgttt attactccct ttaatgtctg
    32161 tttccgagca tttaacaaga tagtccttaa caacttctta agggaaaaaa ataaaattta
    32221 gtgctgtaca gagcgcgtta caacacggtt tactagcagc aaatacggtt atcgcaggga
    32281 tgaaaaaatg cgaattttac tgatagaaga tgacatgctg attggcgacg gcatcaaaac
    32341 gggccttagt aaaatgggtt ttagcgtcga ctggtttaca caaggtcgtc agggaaaaga
    32401 ggcgctttat agcgcacctt atgatgcggt gatcctggat ttaaccttac caggcatgga
    32461 tggtcgcgat attttgcgcg aatggcgaga aaaaggtcag cgtgagccgg tactgatcct
    32521 gaccgcgcgc gatgcgctgg aggaacgtgt agaagggctg cgtctgggag ctgacgatta
    32581 tctgtgtaaa ccttttgcgt tgatagaagt cgccgccagg ctggaagctc tgatgcgccg
    32641 aaccaacggc caggccagca acgagctgcg ccacggtaac gtcatgctcg accccggcaa
    32701 acgtatcgcc acgctggctg gcgaaccctt aacactgaaa ccaaaagaat ttgccctgct
    32761 ggaattactg atgcgtaacg ctggtcgggt actgccgcgc aaactgattg aagagaaact
    32821 gtatacctgg gacgaagagg tcaccagtaa tgccgttgaa gtgcatgtgc atcatctgcg
    32881 acgcaaactc ggtagtgatt ttattcgtac cgtgcatggt attggctaca cattaggtga
    32941 gaaatgaaat ttacccaacg tcttagtctg cgtgtcaggc tgacgctaat ctttttaatt
    33001 ctggcctcgg tgacctggct gctttccagc tttgtcgcct ggaaacaaac aacggataac
    33061 gtcgatgaat tgttcgacac ccaactgatg ctgtttgcca agcggttaag tacgctcgat
    33121 ctcaacgaaa tcaacgcggc ggatcgcatg gcacagacgc caaataaatt aaaacacggt
    33181 catgttgatg acgatgcgct gacctttgcc atctttaccc acgacggcag aatggtcctt
    33241 aacgatggcg ataacggaga agatattccc tatagctatc aacgggaagg ttttgctgac
    33301 gggcaactgg tgggcgataa agatcaatgg cgttttgtct ggatgacctc acctgatggc
    33361 aaatatcgca tcgttgttgg tcaggaatgg gaataccgtg aagacatggc gctggcgatt
    33421 gttgccgggc aattgatccc gtggttggtc gcactgccgg ttatgttaat catcatgatg
    33481 gtactactgg gtcgtgaact cgcgccgctg aacaaactgg cgctggcact acgtatgcgt
    33541 gaccctgact cggaaaaacc actaaacgcg actggcgtac ccagcgaagt gcgaccactg
    33601 gttgagtcgc taaatcaact gttcgcccgc acacatgcga tgatggttcg tgaacgacgc
    33661 tttacctccg acgcagctca cgaacttcgt agcccgttaa cggcgctgaa agtgcaaacc
    33721 gaagttgcgc agctctctga cgatgatccg caggcgcgga aaaaagcact gctccaatta
    33781 cattccggga tcgatcgcgc tactcgtctg gttgatcaac tgctcacgct atcgcggctg
    33841 gactcactgg ataaccttca ggacgtcgcg gagatcccgc ttgaagatct cctgcaatcg
    33901 tcggtgatgg atatttacca cacggcgcag caggcgaaaa ttgacgtgcg actgacactc
    33961 aatgtccagg gcatcaaacg caccggacaa ccgctattgc taagtttgtt ggtgcgaaat
    34021 ttgctggata acgccgtgcg ttacagtcca cagggcagcg tggtggacgt cacgctgaat
    34081 gccgataatt tcatcgtaag ggataacggc cccggtgtga caccagaggc actggcgcga
    34141 attggcgaac gcttctatcg cccacccgga caaaccgcta ccggcagtgg acttgggcta
    34201 tcgattgtcc agcgaatcgc caagctgcat ggcatgaatg ttgagtttgg gaatgcggaa
    34261 caaggtggat ttgaggcgaa ggtaagctgg taagggtaag acttttgcta aattcgcaaa
    34321 agactttgca cattttgcta atttcaccgt accgctctgt gacgtactat agtcggcaaa
    34381 cgtctcacct tgaggttaaa aaatgagcaa catcctgatt atcaacggcg cgaaaaaatt
    34441 cgcccactcc aatggtcaac tgaacgacac cctgaccgaa gtcgcggatg gcacactgcg
    34501 cgacctcggg catgatgttc gcatcgttcg cgccgacagc gactacgatg tcaaagcgga
    34561 agtacaaaac tttctctggg ctgatgtggt gatctggcag atgccaggct ggtggatggg
    34621 cgcgccgtgg acagtgaaaa aatacattga tgatgtattc accgaaggtc acgggactct
    34681 gtatgccagc gatggtcgta cccgcaaaga tccgtcgaaa aaatacggtt ccggcggcct
    34741 ggtacagggc aaaaaatata tgctttctct gacctggaac gcgccaatgg aagccttcac
    34801 cgaaaaagat cagttcttcc acggcgttgg cgttgacggt gtgtatctgc cgttccataa
    34861 agcaaaccaa ttcctcggta tggaaccgct gccgacattt atcgctaatg acgtgataaa
    34921 aatgcctgat gttccccgct atactgaaga atatcgcaag catcttgtgg aaatttttgg
    34981 ttaactagag ctcaggcttt agaaggagtt aaccatgctt accgtaatcg cagaaatccg
    35041 tactcgtcct ggtcaacatc accgtcaggc ggtattggat cagtttgcta aaatcgttcc
    35101 aaccgtactg aaagaagaag gttgccacgg ctatgcgcca atggtggatt gcgctgctgg
    35161 cgtgagtttc cagtctatgg caccggattc tatcgtgatg attgagcagt gggaaagcat
    35221 cgcgcatctt gaagcgcatc tgcaaacccc gcacatgaag gcgtatagcg aagccgtaaa
    35281 aggtgacgtg ctggagatga atattcgtat tctgcagcca gggatttaac attcctgaaa
    35341 agaggccggt gttgctaccg gcctcaggtc agattagcca ttctttcttc ccgttttcag
    35401 cccccgatgg aaatcgttaa ttcgcttaat ggttttaacc gcacgctgcg gcaccgcgga
    35461 agccaccgac cagacaatca tttccaggca caacaatacc gtaccatgta gtggcgtttt
    35521 ccccttttca tccccgcgcg gaacatgaat cacgatactg gcgtctttgc taaaacgtga
    35581 atccagcgcg tttgtcagca aaataacagg gatccccagc cgtctcgctt cacgcagcgt
    35641 tgtcagccct tcccggtgcg cagatttttg cgccatcatg atcagaacat cgccacgttg
    35701 aagcgcaatc agttgctcgg caaggccaat acccgtacgg tttagtgccg tggcaggcaa
    35761 acctatgcgg ttgaatagtc gtgcggtata gtccgccaga atacccgaag cgccaatgcc
    35821 aaaaattgcg acttgccgtg cctgtaccaa caacgctacc gcttgcgcaa gcgcgtggcg
    35881 gttttcaggc tctgacagga cattacaggt atacaaatgc ccttcaagaa caaagtcgat
    35941 tgcggtgtta acatcgctcg tcaagttact caccgtcgta gacatctttt cgctggaact
    36001 aagggctgga ccaagccact gttccagagt gcgtttcaga tcacgcagcc cggcaaaccc
    36061 cagggcctgt atagccctga taactgtggc atcggaagtg ttcaacgtgg ccgcaatttc
    36121 catcgccgtt tgatcgagta ccacttcacg atgctcatta atatagctgg cgacagctct
    36181 taacccaggc gttaactgac tcgcgcgagt gcgaaaacgc tcaccataaa tatcaacccg
    36241 cgttttgtct ttctgaatca tttggcctcc ggtaaaggtc gccccgcaat ttgtgattgt
    36301 atctgcgcag ccatcagccc cagagatgca aaggagttag aaattgcctc ttcacgagaa
    36361 atcagcacat aacgcccgga acgacaggca gtcagaaact gacaccatcc cggcatgact
    36421 ttttccattg ctgccagctc atcctgaggt ttaccaccgg tgtcaccgcg ccaggttgca
    36481 aaaacaaaat cggcatccag ttcaggtaaa cgctccgcgc tcacatccat tcgcccacct
    36541 tcaggaatgc tctcaatcag cggcggaaag cgaaaaccag catcgcgtaa tacccgcccc
    36601 agcgaatgat agctatgcat cacattaatt tttccctgat ttgcctgaat caccgatacc
    36661 gttatttttt ggctgtccag cgtcgccttc agcgcattga tttgtgcctg atagcggcgt
    36721 tcaagtatcg ccagctgcga ctgcgttcct gtcagttctg ccagcttgcg atagatttct
    36781 ggcgcgcccc ctttgagatg atcaatgctc accgtgggcg caattttttc cagtcgttca
    36841 atgggtgtat tacgggtcgg ttctgtgatg atcaggtcag gctttgccgc cacaatcgct
    36901 tcaatatcaa tatcagcggt gccaataaaa gcgatcgatg aattatcaaa gtcgacacct
    36961 gtcaataacg cgcctgatcg aatgaaatgg ctaccgtcag gccgagttcg cccgtggctg
    37021 gcgacaggag gtacaccgag ctcaatcaga ggaatagtga tatcaagatc gtgtaaagag
    37081 acaattctct ttggatgagc aggaacagtg actttgcgcc ctaaatcatc agtaaagacc
    37141 tgaacaggtt ctgtcgcgcg ggcaaagaat gacaacagta tcagcagaga aaaaaataaa
    37201 cgcatataac tcccattaaa aactgttcct gcgctgccag agcagtagca aaaagaatgg
    37261 tccaccaatc aatgaaataa tgattccggc aggcagttgc aaaggcaaaa aagccaaacg
    37321 accaatgcta tccgccagga tgactaacaa cgcccccgat acggcgctcc ccagcagtaa
    37381 tgtcgtctgc ccgccacgta gaataaaacg tgatatatgg ggtgctatca atccgacaaa
    37441 accgatgtta ccaacacacg aaacgcagac ggctgtcagg attattggtg cggcgacacg
    37501 taacaacgcc agacgagaac tattcacccc taacccggtt gcaacctgat gaccaagcag
    37561 agcgatatcc gccgtcctgg cggtgaacag caataaaaga gcggctggca acatccaaca
    37621 ggctgaaata ccgactaaca tccagttagc agcatgcagg cttcccgata accacatcaa
    37681 tgcagtttgt acatctcgaa catctgcagt ggtcataaat acaccgatac cggcggcgaa
    37741 aaaccaggaa acaccaatac caatcagaac aaatcgcggg cgggaaattt cgcgtgcaca
    37801 aaaaatcacg attaaagcca ccaacaagcc accagcaaga ccagccactg gacgccagaa
    37861 cattcccagc attggaaact ggaaaatcag ccacagcacc gccacgctac atccttcttt
    37921 gacgccgatt aaaccaggat cggcaaggcc gttacgcgca atggattgca tcgcggcccc
    37981 cgccatacct aacatggctc cgcatagcaa tgccatgatg actctcggca agcgaatatc
    38041 ctgcacaata taacgtgcgt ctgtgcttac gttttctgga gcaaacaacg ctcgtcctac
    38101 ttccgatgcc gggacaaaaa aagaaccatg catcagaccg aatatcagta ttccaaccgc
    38161 aaacagaaac agcccaccaa gcaacaccag atttttaggg cgtaccagtg tcgaaaactt
    38221 accaacacgt agtggacgta gcccgacgtt cattatccga ctcatttgaa cattctcgtc
    38281 gccataatga caaaaacagg cgcaccaact aacgccgtca tgacaccggt cgctaactca
    38341 tggggcgtga aaagcgtccg agcaataata tctgccagta ataacaaaag cgcgccgaca
    38401 caagctgaaa gagggagcag aacgcgcaaa tccgctgata ccagacgcct gacaatctgc
    38461 ggtactagca gaccgacaaa accaatgggg ccagcaatcg atacggctgc gccacagagc
    38521 aacgcgatcg caagcagagt gaatgttcgc gtgcggagta ccgagacgcc cagcccctgc
    38581 gccatgcgat cacccagtgc caacatgtta agagagggcg caaggtaaat ggctaacaca
    38641 acccccccca gagaaaacca ggcggacgta cccagcgtcg cccaatcctg ccccgctaaa
    38701 tcacctgcta gccatgtccg catcgccagc agtgtttgtt cgtcaagaat caggattgca
    38761 gccgttattg aggatacgaa cgccgatagc gccacgccgc ataaagtaac tttcattggc
    38821 gttagccctg aacgccctgc ggaggaaagc agcaatatca acaggaacaa taacgcgccg
    38881 cccgtagatg ccagcagtgg gcgtccaacc ggaaaggcca gcccaagcgc actggcagca
    38941 acgacagcaa gagcggctcc ggcatttaag cctaaaatat gtggctcccc caatgggtta
    39001 cgaatgacag cctgcaacaa agcaccagca actccaagag aagcaccggt aagcaatgct
    39061 gccgctaaac gcggcaaacg gagtcttaca attacgttgt gatcgaagtt ttgcggttca
    39121 aaatgaagaa atgcctgcaa caccgtttgt ggtgcaataa atcgggcacc aatccctgga
    39181 tataccacgc atcccacaag cagtaaggcg aggaaaatta cagtaaccag ccctgtgcgc
    39241 atcatacttt ttctacacca cgaaacggca tgaagaacgg ctttcccgtc agagtattta
    39301 ttgatgcgtg aacatcaacg tcaaagacag cttttaccag ctcaggcgtg caatgctccc
    39361 cctcattcaa cacacgcacc actttcccct ggcgtaaaaa tatcaacgta tcaccataat
    39421 tgacggcaaa attcagatca tgcaacacca cgacgactgt acgcccgtga tgacgcgtta
    39481 aatcatgtaa taactcaaga atttcaacct gataacgtaa atcaagccag gttgtgggtt
    39541 catccagtag gatataaggc gtcttttgcg ccaacaccat cgcaatccag caacgctggc
    39601 gctgcccacc agagagtttt tcaacaggta aatgagcaaa ctcctgcgta ccggttaatt
    39661 tcagggcctc ttctaccgcc tgctcgtcgg cgtcgctcca ttgacgtatg aaattttgcc
    39721 agggaaaacg cccacgcgac accagttcat aaacggttaa gccctccggc aataatggcg
    39781 actgcggcaa gatcccaagt ctacgggata acgctttcgt tggttgctcg tgaatcgctt
    39841 taccatccag caatattgag ccgcccatgg gttgcaagac acgtgcaatg gtgctgagca
    39901 acgtcgactt tccacagccg tttgccccaa ccagaacggt cattttttct gtgggaacgg
    39961 agaaagaaac accatcaaca atgattttct tgtgataccc ggcagaaaca ttgtcgagga
    40021 tcagcccctg cccttttgcg ttatgtgcca cgtgtacgcc aacttaatca aagaaaaaca
    40081 aaaataaaac tcattctctt ttgagagttt gacgccacaa aatgtagtag tcaagaagtt
    40141 aaaagccata caaaaacaag gtaaataaca ggtgaaatat ttttaaagat aaaaataaa
//
LOCUS       DBFIFM010000015        39035 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.015, whole genome shotgun sequence.
ACCESSION   DBFIFM010000015 DBFIFM010000000
VERSION     DBFIFM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.015"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            393..1652
                     /gene="codB"
                     /locus_tag="N7C74_000754"
     CDS             393..1652
                     /gene="codB"
                     /locus_tag="N7C74_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HGU2430690.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSLIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            1642..2925
                     /gene="codA"
                     /locus_tag="N7C74_000755"
     CDS             1642..2925
                     /gene="codA"
                     /locus_tag="N7C74_000755"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HGU2430691.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKLEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYSIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(3058..3957)
                     /gene="cynR"
                     /locus_tag="N7C74_000756"
     CDS             complement(3058..3957)
                     /gene="cynR"
                     /locus_tag="N7C74_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HGU2430692.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIHLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHLPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAIPLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            4067..4477
                     /gene="cynT"
                     /locus_tag="N7C74_000757"
                     /pseudo
     CDS             4067..4477
                     /gene="cynT"
                     /locus_tag="N7C74_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
     gene            4473..4772
                     /gene="cynS"
                     /locus_tag="N7C74_000758"
                     /pseudo
     CDS             4473..4772
                     /gene="cynS"
                     /locus_tag="N7C74_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
     gene            4778..5959
                     /gene="cynX"
                     /locus_tag="N7C74_000759"
     CDS             4778..5959
                     /gene="cynX"
                     /locus_tag="N7C74_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HGU2430693.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAALTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTIPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSIRGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(6062..6673)
                     /gene="lacA"
                     /locus_tag="N7C74_000760"
     CDS             complement(6062..6673)
                     /gene="lacA"
                     /locus_tag="N7C74_000760"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HGU2430694.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRRKTLMYEFNHSHPSEV
                     DKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPCRVIREINDRDKQYYFRDYKVESSV"
     gene            complement(6739..7992)
                     /gene="lacY"
                     /locus_tag="N7C74_000761"
     CDS             complement(6739..7992)
                     /gene="lacY"
                     /locus_tag="N7C74_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HGU2430695.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAILLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(8044..11118)
                     /gene="lacZ"
                     /locus_tag="N7C74_000762"
     CDS             complement(8044..11118)
                     /gene="lacZ"
                     /locus_tag="N7C74_000762"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HGU2430696.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TNRPSQQLRSLNGEWQFVWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLLSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATLFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLGLNVENPKLWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGEMRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDLVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFHHSDNELLHWTVALDGKPLASGEVPLDVAPQGKQVIE
                     LPELPRLESTGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSAPHAIPQ
                     LTTSETDFCIELDNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTVHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            complement(11241..12323)
                     /gene="lacI"
                     /locus_tag="N7C74_000763"
     CDS             complement(11241..12323)
                     /gene="lacI"
                     /locus_tag="N7C74_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HGU2430697.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPKT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            complement(12360..13313)
                     /locus_tag="N7C74_000764"
     CDS             complement(12360..13313)
                     /locus_tag="N7C74_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HGU2430698.1"
                     /translation="MRPLTVAIIAVAGFSPFHLSVPFIVFSEKMAEKKRFHVIICAEK
                     PGNVDSADGFSVTATHDYTAVIQADIVIIPYWGTITQKPPQKLLEALTTARDNGAQIV
                     GLCLGTFVLGYAGLLKNKRAATHWEFEREFQARFPQTHLDINALYVDDDGIITSAGTA
                     AALDCCLYIVRQHFGSDYANHIARRMVVPPYRTGGQAQFIEQPVPKNTHDERINLLLD
                     YLRQNIAQQHDLDSLAQRVMMSRRTLTRHFMKATGSSIAEWLITERLRRSQELLGSSQ
                     LPVERIAAEVGFLSPVTWRQHFKSHFGVSPAEWRKTFRGMA"
     gene            complement(13333..14109)
                     /locus_tag="N7C74_000765"
     CDS             complement(13333..14109)
                     /locus_tag="N7C74_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGU2430699.1"
                     /translation="MKITQIRNATQIIQYAGKTFLIDPLLAPKGSYPGFAGTARAEIR
                     NPTVELPCDLTTLLNVDALIVTHLHEDHWDETASRVVPKDKLIYVQNEQDAESLNKQG
                     FTHLTVLTDETKFGDITLHKTTGQHGSDRTYADPAMAKRLGAACGVVFQHPTEKTLYL
                     AGDTLWRDDVEATMLTFQPEVVVLNAGFAHVLGHGAIIMGAEDILKTHFTLPEAQIIA
                     THMEAVNHCLLTRDALKEYARDNQIIEFVNVPEDGETLTF"
     gene            complement(14224..15057)
                     /gene="mhpR"
                     /locus_tag="N7C74_000766"
     CDS             complement(14224..15057)
                     /gene="mhpR"
                     /locus_tag="N7C74_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HGU2430700.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15248..16912
                     /gene="mhpA"
                     /locus_tag="N7C74_000767"
     CDS             15248..16912
                     /gene="mhpA"
                     /locus_tag="N7C74_000767"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HGU2430701.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     LREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALSTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            16914..17858
                     /gene="mhpB"
                     /locus_tag="N7C74_000768"
     CDS             16914..17858
                     /gene="mhpB"
                     /locus_tag="N7C74_000768"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HGU2430702.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            17876..18742
                     /gene="mhpC"
                     /locus_tag="N7C74_000769"
     CDS             17876..18742
                     /gene="mhpC"
                     /locus_tag="N7C74_000769"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HGU2430703.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARA"
     gene            18752..19561
                     /gene="mhpD"
                     /locus_tag="N7C74_000770"
     CDS             18752..19561
                     /gene="mhpD"
                     /locus_tag="N7C74_000770"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HGU2430704.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAAAFSSAAPKGSLS"
     gene            19558..20508
                     /gene="mhpF"
                     /locus_tag="N7C74_000771"
     CDS             19558..20508
                     /gene="mhpF"
                     /locus_tag="N7C74_000771"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HGU2430705.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHDQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVDQLNVNMVTCGGQATIPMVAAVSRVVRVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            20505..21518
                     /gene="mhpE"
                     /locus_tag="N7C74_000772"
     CDS             20505..21518
                     /gene="mhpE"
                     /locus_tag="N7C74_000772"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HGU2430706.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHRYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAVDKLGWQHGADLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            21694..22905
                     /gene="mhpT"
                     /locus_tag="N7C74_000773"
     CDS             21694..22905
                     /gene="mhpT"
                     /locus_tag="N7C74_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HGU2430707.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRLRMQPCADA"
     gene            23007..23546
                     /gene="yaiL"
                     /locus_tag="N7C74_000774"
     CDS             23007..23546
                     /gene="yaiL"
                     /locus_tag="N7C74_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HGU2430708.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(23671..24504)
                     /gene="fghA"
                     /locus_tag="N7C74_000775"
     CDS             complement(23671..24504)
                     /gene="fghA"
                     /locus_tag="N7C74_000775"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HGU2430709.1"
                     /translation="MELIEKHASFGGWQNVYRHYSASLKCEMNVGVYLPPKAAGEKLP
                     VLYWLSGLTCNEQNFITKSGVQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMNHFPATAKKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKEAWLDYHPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTQNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(24597..25706)
                     /gene="frmA"
                     /locus_tag="N7C74_000776"
     CDS             complement(24597..25706)
                     /gene="frmA"
                     /locus_tag="N7C74_000776"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HGU2430710.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(25741..26016)
                     /gene="frmR"
                     /locus_tag="N7C74_000777"
     CDS             complement(25741..26016)
                     /gene="frmR"
                     /locus_tag="N7C74_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HGU2430711.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            complement(26176..27222)
                     /gene="fbpC"
                     /locus_tag="N7C74_000778"
     CDS             complement(26176..27222)
                     /gene="fbpC"
                     /locus_tag="N7C74_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HGU2430712.1"
                     /translation="MSQKNFVELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKEALAMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPVQGSGTVGVR
                     PEAITLSNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADAA"
     gene            complement(27234..29312)
                     /locus_tag="N7C74_000779"
     CDS             complement(27234..29312)
                     /locus_tag="N7C74_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2430713.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFVLLPSWSLDYGLLESTS
                     DEILGAYGWSHFNISWLWYLLPSLLLVRPFRQATREQRSRHYFDAGWALLCMAFIVIS
                     ATVAGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGELAPLAFMTVLSQAHIVQVIVNSIVLSIAVGIGCAFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYVTEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCIQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILAIWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPVYLTGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(29381..30412)
                     /locus_tag="N7C74_000780"
     CDS             complement(29381..30412)
                     /locus_tag="N7C74_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU2430714.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(30409..31725)
                     /gene="uhpC"
                     /locus_tag="N7C74_000781"
     CDS             complement(30409..31725)
                     /gene="uhpC"
                     /locus_tag="N7C74_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HGU2430715.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLSANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWPGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(31798..33339)
                     /locus_tag="N7C74_000782"
     CDS             complement(31798..33339)
                     /locus_tag="N7C74_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HGU2430716.1"
                     /translation="MSRNLRHWVILLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALVILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPALLAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNICKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMRERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(33339..33968)
                     /locus_tag="N7C74_000783"
     CDS             complement(33339..33968)
                     /locus_tag="N7C74_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGU2430717.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRAQKPQFRAIILSIYDAPIFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFELLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
     gene            34271..35233
                     /gene="tauA"
                     /locus_tag="N7C74_000784"
     CDS             34271..35233
                     /gene="tauA"
                     /locus_tag="N7C74_000784"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU2430718.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            35246..36013
                     /gene="tauB"
                     /locus_tag="N7C74_000785"
     CDS             35246..36013
                     /gene="tauB"
                     /locus_tag="N7C74_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HGU2430719.1"
                     /translation="MLQISHLYADYGGKPVLEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     IQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            36010..36837
                     /gene="tauC"
                     /locus_tag="N7C74_000786"
     CDS             36010..36837
                     /gene="tauC"
                     /locus_tag="N7C74_000786"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HGU2430720.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            36834..37685
                     /gene="tauD"
                     /locus_tag="N7C74_000787"
     CDS             36834..37685
                     /gene="tauD"
                     /locus_tag="N7C74_000787"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HGU2430721.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(37792..38766)
                     /gene="hemB"
                     /locus_tag="N7C74_000788"
     CDS             complement(37792..38766)
                     /gene="hemB"
                     /locus_tag="N7C74_000788"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HGU2430722.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         9002 a        10257 c        10710 g         9066 t
ORIGIN      
        1 cgacaccgtg ctcagtgcct gatgcgacgc tgatgcgtct tatcatgcct acaggcctga
       61 accgtaggtc ggataaggcg ctcgcgccgc atccgacacc gtgcgcagat gcctgatacg
      121 acgcaggcgc gtcttatcat gcctacaggc ctgaaccgta ggtcggataa ggcgctcgcg
      181 ccgcatccga caccgtgcgt agttgcctga tgcgacgctg acgcgtctta tcatgcctac
      241 ccactgtttt tacaccgata attttccccc cacctttttg cactcattca tataaaaaat
      301 atatttcccc acgaaaacga ttgcttttta tcttcagatg aatagaatgc ggcggatttt
      361 ttgggtttca aacagcaaaa agggggaatt tcgtgtcgca agataacaac tttagccagg
      421 ggccggtccc gcagtcggcg cggaaagggg tattggcatt gacgttcgtc atgctgggat
      481 taaccttctt ttccgccagt atgtggaccg gcggcactct cggaaccggt cttagctatc
      541 atgatttctt cctcgcagtt ctcatcggta atcttctcct cggtatttac acttcatttc
      601 tcggttacat tggcgcaaaa accggcctga ccactcatct tcttgctcgc ttctcgtttg
      661 gtgttaaagg ctcatggctg ccttcactgc tgctgggcgg cacccaggtt ggctggtttg
      721 gcgtcggtgt ggcgatgttt gctattccgg tgggcaaggc aaccgggctg gatattaatt
      781 tgctgattgc cgtttccggt ttactgatga ccgtcaccgt cttctttggc atttcggcgc
      841 tgacggttct ttcgttgatt gcggttccgg ctatcgcctg tcttggcggt tattccgtgt
      901 ggctggccgt taacggcatg ggcggcctgg acgccttaaa agcggtcgtt cccgcacaac
      961 cgttagattt caatgtcgcg ctggcgctgg ttgtggggtc atttatcagt gcgggcacgc
     1021 tcaccgctga ctttgtccgc tttggtcgca atgccaaact ggcggtgctg gtggcgatgg
     1081 tggccttttt cctcggcaac tcgttgatgt ttattttcgg tgcagcgggc gctgcggcac
     1141 tggggatggc ggatatctct gatgtgatga ttgctcaggg cctgctgttg cctgcgattg
     1201 tggtgctggg gctaaatatc tggaccacca acgataacgc gctctatgcg tcgggtttag
     1261 gtttcgccaa cattaccggg atgtcgagca aaaccctttc ggtaatcaac ggtattatcg
     1321 gtacggtctg tgcattatgg ctgtataaca attttgtcgg ctggttgacc ttcctttcgg
     1381 cagctattcc tccagtgggt ggcgtgatca ttgccgacta tctgatgaac cgtcgccgct
     1441 atgagcactt tgcgaccacg cgtatgatga gtgtcaattg ggtggcgatt ctggcggtcg
     1501 ccctggggat tgctgcaggc cactggttac cgggaattgt tccggtcaac gcggtattag
     1561 gtggcgcgct gagctatctg atccttaacc cgattttgaa tcgtaaaacg acagcagcaa
     1621 tgacgcatgt ggaggctaac agtgtcgaat aacgctttac aaacaattat taacgcccgg
     1681 ttgccaggca aagaggggct gtggcagatt catctgcagg acggaaaaat cagcgccatt
     1741 gatgcgcaat ccggcgtgat gcccataact gaaaacagcc tggatgccga acaaggttta
     1801 gttataccgc cgtttgtgga gccacatatt cacctggaca ccacgcaaac cgccggacaa
     1861 ccgaactgga atcagtccgg cacgctgttt gaaggcattg aacgctgggc cgagcgcaaa
     1921 gcgttattaa cccatgacga tgtgaaacaa cgcgcatggc aaacgctgaa atggcagatt
     1981 gccaacggca ttcagcatgt gcgtacccat gtcgatgttt cggatgcaac gctaactgcg
     2041 ctgaaagcaa tgctggaagt gaagctggaa gtcgcgccgt ggattgatct gcaaatcgtc
     2101 gccttccctc aggaagggat tttgtcgtat cccaacggtg aagcgttgct ggaagaggcg
     2161 ttacgcttag gggcagatgt agtgggggcg attccgcatt ttgaatttac ccgtgaatac
     2221 ggcgtggagt cgctgcataa aaccttcgcc ctggcgcaaa aatacgaccg tctcatcgac
     2281 gttcactgtg atgagatcga tgacgagcag tcgcgctttg tcgaaaccgt tgctgccctg
     2341 gcgcaccgtg aaggcatggg cgcgcgagtc accgccagcc acaccacggc aatgcactct
     2401 tataacgggg cgtatacctc acgtctgttc cgcttgctga aaatgtccgg tattaacttt
     2461 gtcgccaacc cgctggtcaa tattcatctg caaggacgat tcgatacgta tccaaaacgt
     2521 cgcggcatca cgcgcgttaa agagatgctg gagtccggca ttaacgtctg ctttggtcac
     2581 gatgatgtct tcgatccgtg gtatccgctg ggaacggcga atatgctgca agtgctgcat
     2641 atggggctgc atgtttgcca gctgatgggc tatgggcaga ttaacgatgg cctgaattta
     2701 atcacccacc acagcgccag gacgttgaat ttgcaggatt acagcattgc cgccggaaac
     2761 agcgccaacc tgattatcct gccggctgaa aatggatttg atgcgctgcg ccgtcaggtt
     2821 ccggtacgtt attcggtacg tggcggcaag gtgattgcca gcacacaacc ggcacaaacc
     2881 accgtatatc tggagcagcc ggaagccatc gattacaaac ggtgatcggc tgggctgtgt
     2941 gttgacaaag cgagcgtagt ttatgccgga tgcggcttga acgccttatc aggcctacgt
     3001 agagcactga actcgtaggc ctgataagcg taacgcatca ggcaattgtg tcagtggtta
     3061 ccgcgattca ttctcgccaa catccgcaca ttcctccaac gccatgtgca aaaatgcctt
     3121 cgcggcggct gtctgccagc tatttttccg ccgcaacaaa accgccgttc tctccagtag
     3181 tggcggggca agaggaatcg ctttaagccc gtcatgttgt gtggcaatcg ctgctggtaa
     3241 caatgtggaa agggaagtgc ggcgaatcag ctccagaacc gcgctaattg agttcgcctc
     3301 aatgaccacc tgtggatgca gccccgcttt ctcgcagtag tggtcaatct gctctctggt
     3361 ggcaaattcc gcgctgagca ggaccagttt ttcatcatgc aagcgactca acgccacctg
     3421 ttcacaggca gccagcggat gatgttgcgc cacgactaac gctaaacttt ctgtcagtaa
     3481 aggaattgcc tccagctccg gcaaatgcac aggcgcgaag gcaatcccaa cgtccagctc
     3541 gtcgcgacaa agcagatctt cgattttctc ctgcgacatt tcctgtagct ggagcgtgat
     3601 gccggggtag cgcgcataga aatccgccat taaggggccg ataaagtagc tcgtaaaggt
     3661 gggggtgacg gcgatacgca gcgatcctct cgtcagatcg gcaacatcat gaatcgcccg
     3721 tttacccgct cccagttcct gtaacgcccg gctggcgtac tgtcgccaga cttctcctgc
     3781 atcagtgaga tgaatcgttc gcccgctacg gtcaaacagc ggcacgccta aactctcctc
     3841 taactggcga atctgctggg aaagcgcagg ctgagagacg tgcaacgcac tggcggcacg
     3901 ggtgaagctg ccatgttcag ccacggcaag aaaataattg atatgtcgag agagcattcg
     3961 ccaacctata agtaaattca atagaactca tcataaatga gacttttacc ttatgacaat
     4021 cggcgagtag tctgcctctc attccagaga cagatagagg ttaacggtga aagagattat
     4081 tgatggattc cttaaattcc agcgcgaggc atttccgaag cgggaagcct tatttaaaca
     4141 gttggcgaca cagcaaagcc cgcgcacact ttttatctcc tgctccgaca gccgtctggt
     4201 ccctgagctg gtgacgcaac gtgagcctgg cgatcttttc gttattcgca atgcgggcaa
     4261 tatcgtccct tcctacgggc cggaacccgg tggcgtttct gcttcggtgg agtatgccgt
     4321 cgctgcgctt cgggtatctg acattgtgat ttgcggtcat tccaactgtg gcgcgatgac
     4381 cgccattgcc agctgtcagt gcatggacca tatgcctgcc gtctcccact ggctgcgtta
     4441 tgccgattca gcccgcgtcg ttaatgaggc gcgcccgcca ggtcggggcg aagctggatc
     4501 tcgacgaaga cgccattcta ctgttacaga tgatcccact gcgtggctgc attgatgacc
     4561 gtattccaac cgacccaacg atgtatcgtt tctatgaaat gttgcaggtg tacggcacaa
     4621 ccctgaaagc gttggttcat gagaaatttg gcgatggcat tatcagcgcg attaacttca
     4681 aactcgacgt taagaaagtg gcggacccgg aaggtggcga acgtgcggtc atcaccttag
     4741 atggtaaata tctgccgacc aaaccgttct gacagccatg cgtaaccatc aaaagacgtt
     4801 cacgatgctg ctggttctgg tgctgattgg tcttaatatg cgaccactgc tcacctccgt
     4861 cgggccactg ctaccgcaat tgcgccaggc aagcggaatg agctttagcg tggctgccct
     4921 gttgaccgct ctgccggtgg ttaccatggg cgggctggcg ctggccggaa gctggcttca
     4981 tcagcatgtc agcgaacgtc gcagcgtcgc catcagtttg ttactgattg ccgtcggtgc
     5041 attgatgcgt gagctttacc ctcaaagtgc gctgctgctt agcagcgcac tgctcggtgg
     5101 ggtggggatc ggcatcattc aggcggtgat gccttcggtg attaaacggc ggtttcagca
     5161 gcgcacgcca ctggtgatgg ggctgtggtc cgcggctctg atgggcggcg gtgggcttgg
     5221 tgccgccctg acaccctggt tagttcaaca tagcgaaacc tggtatcaaa cactcgcctg
     5281 gtgggcgctg cctgccgttg ttgcgctctt tgcctggtgg tggcaaagca cccgcgaggt
     5341 cgcctcttcc cacaagacaa caaccattcc ggttcgcgtg gttttcactc ctcgcgcgtg
     5401 gacgctgggt gtttacttcg gtctgattaa cggtggttac gccagcctga ttgcctggtt
     5461 acccgccttc tatattgaga ttggtgccag cgcgcagtac agcggttcct tgctggcatt
     5521 gatgacgctt gggcaagccg caggagcttt gctgatgcct gctatggctc gccatcagga
     5581 tcggcgcaaa ctgttaatgc tggcgctggc gttacaactg gtgggtttct gcggctttat
     5641 ctggctgccg ctgcaattgc cggtattgtg ggcgatggtg tgtggtttag gtctgggcgg
     5701 cgcatttccg ctctgtttgc tgctggcgct cgatcactct gtgcaaccgg ctattgctgg
     5761 caagctggtg gcgtttatgc agggaatcgg ttttatcatc gccgggcttg ccccgtggtt
     5821 ttctggcgtg ctgcgtagta tcaggggcaa ttacttgatg gactgggcgt ttcatgcgct
     5881 gtgcgtcgta gggctgatga ttataaccct gcgttttgca ccagcacgtt ttccgcagct
     5941 gtgggtcaaa gaggcatgat gcgacgcttg tttctgcgct ttgttcacgc cggatgcggc
     6001 ctacatagat cactgaattt gtaggcctga taagcgaagc gtatcaggca atttttataa
     6061 tttaaactga cgattcaact ttgtaatctc tgaaataata ttgcttatcc cggtcgttta
     6121 tttcgcgaat aacccgacaa ggaacgccag ccgccacgac gtttggtgga atgtcttttg
     6181 tgacgatact acccgcgcca ataacagaat tatccccaat ggtgacgcct ggattaataa
     6241 ccacatgact tccgatccag acgttattgc caatcgttat cggaaaagag tacatctcac
     6301 cgttttttct caattcatgg tgtacagggt gtcccgtaac ggaaagagta acgttgggtg
     6361 caatcaatac gttatcaccg attgttaccg tgtagtcatc gacaatggtt aaattgaaat
     6421 ttgcataaaa attgcggcct atatggatgt tggaaccgta agagaaatag acggggggtt
     6481 ctacccaggc gttttcccct accgtggcaa acatttcttt aatcaggctt tctcttttat
     6541 caacttctga tggatgcgag tgattaaact catacattaa cgtttttcta cgaagtcttt
     6601 tttcaggtaa gccttcgcac atatcggtaa atagcttgcc tgcttttatt ctttctgtca
     6661 tcgacatgtt cattgcgatc attccgttat gatatgttgg tcggataagg cgctcgcgcc
     6721 gcatccgaca ttgattgctt aagcgacttc attcacctga cgacgcagta gagaaagcgg
     6781 gccggggccg ctaagcgtga acacggaaat taaggtgaag cccagcgcca ccagacccag
     6841 caccagataa gcgccctgga aaccgatgct ttcatacata ttacccgcta gtacagacat
     6901 aaaaatcatc gccagttgct taaagaagca gaaacagacc agataaatcg tcgctgaaaa
     6961 acgcacttca aactggctgg taatatattt aaagcagccc accagcagga acggtacttc
     7021 aaacatatgc agcgttttca gaataaccac ttccagcgct gaggtggcga acgatgagcc
     7081 aataatacgt acagacataa tagtgccagc cagcagcagg gcatttttcc caccgatgcg
     7141 attaatgatc agtggcgcaa agaacataat tgaggcgtta agtaattcgc ccattgtcgt
     7201 tacgtagcca aatacccgcg taccctgttc accggtggca aagaaagaag taaagaaatt
     7261 agcaaactgt tggtcaaaaa catcgtaggt gcaggaaacg ccaataacat acagtgacaa
     7321 aaaccacagt tttggctgtc tgaacagttc cagcgccagt ttaaggctaa atgccgaatg
     7381 gttggcacct accgcattgg caaccgtggc ggaagagggc gcatccgttt tggcgaaaaa
     7441 gagtaaaatg gcgaggatga gtgcacagcc agaacccagc cagaaaacga actgattatt
     7501 gatggtgaac atgatgccga caatcgaggc acacagcgcc cagccaacac agccaaacat
     7561 ccgcgcgcga ccaaattcga aattactgcg acggctgact ttctcgataa atgcctctac
     7621 tgcgggcgca ccggcgttaa aacaaaagcc aagataaata ccaccaacaa tcgatcctac
     7681 taaaatgttg tattgtaaca gtggcccgaa gataaaaata aagaacggcg caaacatcac
     7741 taacatgccg gtaataatcc acagcaggta tttgcgcagc ccgagtttgt cagaaagcag
     7801 accaaacagc ggttggaata atagcgagaa cagagaaata gcagcaaaaa taatacccgt
     7861 atcacttttg ctgatatggt tgatgtcatg tagccaaatc gggaaaaacg ggaagtaggc
     7921 tcccatgata aaaaagtaaa agaaaaagaa taaaccgaac atccaaaagt ttgtgttttt
     7981 taaatagtac ataatggatt tccttacacg aaatacgggc agacatggcc tgcccggtta
     8041 ttattatttt tgacaccaga ccaactggta atggtagcga ccggcgctca gctggaattc
     8101 cgccgatact gacgggctcc aggagtcgtc gccaccaatc cccatatgga aaccgtcgat
     8161 attcagccat gttccttctt ccgcgtgcag cagatggcga tggctggttt ccatcagttg
     8221 ttgttggctg tagcggctga tgttgaactg gaagtcgccg cgccactggt gtgggccata
     8281 attcaattcg cgcgtcccgc agcgcagacc gttttcgctc gggaagacgt acggggtata
     8341 catgtctgac aatggcagat cccagcggtc aaaacaggcg gcagtaaggc ggtcgggata
     8401 gttttcttgc ggccctaatc cgagccagtt tacccgctct gctacctgcg ccagctggca
     8461 ggtcaggcca atccgtgccg gatgcggtgt atcgctcgct acttcaacat caacggtaat
     8521 cgccatttga ccactaccat caatccggta ggttttccgg ctaataaata aggtttttcc
     8581 ctgatgctgc catgcgtgga cagtggtaat cagcaccgcg tcggcaagtg tatctgccgt
     8641 gcactgcaac aacgccgctt ctgcctggta atggcccgca gccttccagc gttcgaccca
     8701 ggcgttaggg tcaatgcggg tcgcttcact tacgccaatg tcgttatcca gcggtgcgcg
     8761 ggtgaactga tcgcgcagcg gcgtcagcag ttgtttttta tcgccaatcc acatctgtga
     8821 aagaaagcct gactggcggt taaattgcca acgcttatta tccagctcga tgcaaaaatc
     8881 cgtttcgctg gtggtcagtt gcgggatggc gtggggcgcg gaggggagtg tcacgctgag
     8941 gttttccgcc agacgccact gctgccaggc gctgatgtgt ccggcttctg accatgcggt
     9001 cgcgttcggt tgcactacgt gtaccgttag ccagagttgc ccggtgctct ccagtcgcgg
     9061 tagttcaggc aattcaatta cctgtttacc ttgcggagcg acatccagag gcacttcacc
     9121 gcttgccagc ggcttaccat ccagcgccac cgtccagtgc aggagctcgt tatcgctatg
     9181 atggaacagg tattcgctgg tcacttcgat ggttcgcccg gataaacgga actggaaaaa
     9241 ctgctgctgg tgttttgctt ccgtcagcgc cggatgcggc gtgcggtcgg caaagaccag
     9301 accgttcatg cagaactggc gatcattcgg cgtatcgcca aaatcaccgc cgtaagccga
     9361 ccacggattg ccattttcat catatttaat cagcgactga tccaccaagt cccagacgaa
     9421 gccgccctgt aaacgggggt actgacgaaa cgcctgccag tatttagcga agccgccaag
     9481 actgttaccc atcgcgtggg cgtattcgca aaggatcagt gggcgcattt ctccaggcag
     9541 cgaaagccat tttttgatgg accatttcgg cacagccggg aagggctggt cttcatccac
     9601 gcgcgcgtac atcgggcaaa taatatcggt tgcggaggtg tcggctccgc cgccttcata
     9661 ctgcaccggg cgggatggat cgacagattt aatccagcga tagagtgcgt cgtgattagc
     9721 gccgtggcct gactcattcc ccagcgacca gatgatcaca ctcgggtgat tacgatcgcg
     9781 ctgcaccatt cgtgttactc gttcgctcat ggccggtaac cagcgcggat catcggtcag
     9841 acgattcatt ggcaccatgc cgtgggtttc aatattggct tcatccacca catacaggcc
     9901 gtagcggtcg cacagggtgt accacagcgg atggttcgga taatgcgaac agcgcacggc
     9961 gttaaagttg ttctgcttca ttagcaggat atcctgcacc atcgtctgct catccatgac
    10021 ctgaccatgc agaggatgat gctcgtgacg gttaacgccg cgaatcagca acggcttgcc
    10081 gttcagcagc agcagaccat tttcaatccg cacctcgcgg aaaccgacat cgcaggcttc
    10141 tgcttcaatc agcgtgccgt cggcggtgtg cagttcaacc accgcacgat agatattcgg
    10201 gatttcggcg ctccacagtt tcgggttttc gacgttcaga cctagtgtga cgcgatcggc
    10261 ataaccgcca cgctcatcga taatttcacc gccgaaaggc gcggtgccgc tggcgacctg
    10321 cgtttcaccc tgccacaaag aaaccgtcac ccgcagctca tcgcgcagct cgccgtacat
    10381 ctgaacttct gcctccagta ccgcgcggct aaaatcatca ttaaagagag tggcaacatg
    10441 gaaatcgctg atttgtgtgg tcggtttgtg cagcaacgag acgtcacgga aaatgccgct
    10501 catccgccac atatcctgat cttccagata actgccgtca ctccagcgca gcaccatcac
    10561 cgcgaggcgg ttttctccgg cgcgtaaaaa tgcgctcagg tcaaattcag acagcaaacg
    10621 actgtcctgg ccgtaaccga cccagcgccc gttgcaccac agatgaaacg ccgagttaac
    10681 gccatcaaaa ataattcgcg tctggccttc ctgtagccag ctttcatcaa cattaaatgt
    10741 gagcgagtaa caacccgtcg gattctccgt gggaacaaat ggcggattga ccgtaatggg
    10801 atatgtcacg ttggtgtaga tgggcgcgtc gtaaccgtgc atctgccagt ttgaggggac
    10861 gacgacagta tcggcgtcag gaagatcgca ctccagccag ctttccggaa ccgcttctgg
    10921 tgccggaaac cagacaaatt gccactcacc attcaggctg cgcaactgct gggaagggcg
    10981 attggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
    11041 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaatacga cggccagtga
    11101 atctgtaatc atagtcatag ctgtatcctg tgtgaaattg ttatccgctc acaatttcac
    11161 acaacatacg agccggacgc atagagtgta aagcgtgggg tgcctaatga gtgagctgac
    11221 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacttg tcgtgccagc
    11281 tgcattaatg aatcggccaa cgcgcgggga gaagcggttt gcgtcttggg cgccagggtg
    11341 gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga
    11401 gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg
    11461 gtggttaacg gcgggatata acacgagctg tcttcggtat cgtcgtatcc cactaccgag
    11521 atatccgcac caacgcgcaa cccggactcg gtaatggcgc gcattgcgcc cagcgccatc
    11581 tgatcgttgg caaccagcat cgcagtggga acgatgccct catttagcat ttgcatggtt
    11641 tgttgaaaac cggacatggc actccagtcg ccctcccgtt ccgctatcgg ctgaatttga
    11701 ttgcgagtga gatatttatg ccagcccgcc agacgcagac gcgccgagac agaacttaat
    11761 gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt
    11821 cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gagtctggtc agagacatca
    11881 agaaataacg ccggaacatt agcgcaggca gcttccacag caatggcatc ctggtcatcc
    11941 agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg cactgccgct
    12001 ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga
    12061 tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag
    12121 gtggcaacgc caatcagcaa cgactgtttc cccgccagtt gttgtgccac gcggttggga
    12181 atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg
    12241 ctggcctgat tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca
    12301 tcgtatagcg ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat
    12361 catgccatac cgcgaaaggt tttgcgccat tcggcggggc tgacgccgaa gtgagattta
    12421 aaatgctgac gccaggtcac aggtgagaga aaacccacct cagccgctat ccgctcaacg
    12481 ggcaactgac tggatcccaa cagttcctgg ctacggcgta agcgttcagt aatgagccat
    12541 tcggcgatac tcgaaccggt cgctttcata aaatggcgag ttaatgtgcg gcgactcatc
    12601 attactcgct gcgccagcga gtcgagatca tgctgttgcg caatgttttg ccgcaggtaa
    12661 tccagcagga ggtttatgcg ttcatcatgg gtatttttcg gcaccggttg ctcaataaac
    12721 tgcgcctgac cgccggtgcg atatggcggt acgaccatcc gtcgggcaat atggttagca
    12781 tagtcgctgc caaaatgttg ccgaacaata tacaaacagc aatccagcgc cgcggcagta
    12841 ccggcagagg taataatgcc gtcatcgtct acgtacaacg cgttaatatc cagatgtgtt
    12901 tgtggaaaac gtgcctgaaa ttcacgctcg aactcccagt gcgtggcggc acgcttattt
    12961 ttcagtaaac ctgcatagcc gagcacaaac gtgcccaggc aaagcccgac aatctgtgcg
    13021 ccgttatccc gtgcggtcgt taaggcttcc agcagttttt gtggcggttt ttgtgtaatg
    13081 gttccccagt aaggaattat cacaatatct gcctggatga ctgcggtata gtcatgggtg
    13141 gcggttacgg aaaacccatc ggcagagtcc acgtttcccg gcttttcagc gcaaataatc
    13201 acgtgaaagc gttttttctc cgccatcttt tcactaaaca cgataaacgg tacggaaagg
    13261 tgaaaaggac taaaaccggc aacggcgata atggcaacag ttagcgggcg cataacactc
    13321 tttatctccg aattagaatg tcaacgtttc accatcttct ggtacgttca caaattcgat
    13381 aatttgatta tctctggcgt attccttgag cgcatcacgc gtcagcaaac agtggtttac
    13441 ggcctccata tgtgtcgcga taatttgcgc ttctgggagg gtaaaatgtg ttttgagaat
    13501 atcttctgct cccataatta tcgcgccatg ccccagaaca tgcgcaaatc cggcattcaa
    13561 cacaaccact tctggttgga aagttagcat tgtagcctcg acgtcgtcac gccagagggt
    13621 atcgccagcc agatagagcg ttttctctgt cgggtgctgg aaaacaacgc cgcaagctgc
    13681 gcctaaacgc ttcgccatcg ccgggtcggc ataggtgcga tcggagccgt gctgccctgt
    13741 cgttttatgc agagtaatat cgccaaattt agtctcgtcg gtaagtacgg taagatgcgt
    13801 aaatccttgt ttattcagtg attcagcatc ttgctcattt tgcacataaa tcagtttatc
    13861 tttagggacg acgcgggagg ctgtttcgtc ccagtgatct tcatgcaaat gggtcacgat
    13921 tagggcgtca acgttgagta acgttgttaa atcgcacggt aattctaccg ttggattgcg
    13981 gatttctgcg cgtgccgttc cggcaaaacc cggataactt cctttaggcg cgagtaacgg
    14041 gtcgataaga aatgttttgc cagcgtattg gataatttgg gtggcattac gaatttgcgt
    14101 aattttcatg atttgctcca catcacagta ttgatgtcat tagcgtagcg tgcagcccgt
    14161 actgtgcatg tgggcaaatg ggcgaataac gatgagaatg ggccaaatta cacattggcg
    14221 taactagcgt aaatgcacgc cgcttcgccg tccggccacc agaatagcct gcgattcaac
    14281 cccttcttcg atctgttttg ctacccgttg tagtgccgga agatgctttt ccgctgcctg
    14341 ttcgatggtc attgcgctcg ccatatacac cagattcaga cagccaatca cccgttgttc
    14401 actgcgcagc ggtacggcga tagaggcgat cttctcctcc tgatcccagc cgcggtagtt
    14461 ctgtccgtaa ccctctttgc gcgcgcgcgc cagaatggct tccagcttta acggttctcg
    14521 tgccagttga tagtcatcac cggggcggga ggctaacatc tcgattaatt ccttgcgttc
    14581 ttgttccggg caaaaggcca gccaggtcag gcccgaggcg gttttcagaa gcggtaaacg
    14641 ccgcccgacc attgcccggt gaaaggataa gcggctgaaa cggtgagtgg tttcgcgtac
    14701 caccattgca tcaacatcca gcgtggacac atctgtcggc cataccactt cgcgcaacag
    14761 atcgcccagc agtggggccg ccagtgcaga aatccactgt tcgtcacgaa atccttcgct
    14821 taattgccgc actttgatgg tcagacgaaa actatcatcg gagggactac gccggacata
    14881 tccctcttcc tgcagcgtct ccagcagtcg ccgcacagtg gtgcgatgca ggccgctgag
    14941 ttccgccagc agcccgacgc tggcaccgcc gtcaagttta tttaacatat ttaataacat
    15001 tagaccgcgg gttaagccgc gcacggtttt gtattccgtc tgctcattgt tctgcatatt
    15061 aattgacatt tctatagtta aaacaacgtt gtgcacctgg tgcacattcg ggcatgtttt
    15121 gattgtagcc gaaaacaccc tttctatact gagcgcacaa taaaaaatca tttacatatt
    15181 tttaacaaaa taagttgcgc tgtactgtgc gcgcaacggc attttgtccg agtcgtgagg
    15241 tactgaaatg gcaatacaac accctgacat ccagcctgct gttaaccata gcgttcaggt
    15301 ggcgatcgct ggtgccggtc cggtcgggct gatgatggcg aactatctcg gccagatggg
    15361 cattgacgtg ctggtggtgg agaaactcga taagttgatc gactacccgc gtgcgattgg
    15421 tattgatgac gaggcgctgc gcaccatgca gtcggtcggc ctggtcgata atgttctgcc
    15481 gcatactacg ccgtggcacg cgatgcgttt tctcaccccg aagggccgct gttttgctga
    15541 tattcagcca atgaccgatg aatttggctg gccgcgccgt aacgccttta ttcagccgca
    15601 ggtcgatgcg gtgatgctgg aaggggtgtc gcgttttccg aatgtgcgct gcttgttttc
    15661 ccgcgagctg gaggccttca gtcagcaaga tgacgaagtg accttgcacc tgaaaacggc
    15721 agaagggctg cgggaaatag tcaaagccca gtggctggtg gcctgtgatg gtggggcaag
    15781 ttttgtccgt cgcaccctga atgtgccgtt tgaaggtaaa actgcgccaa atcagtggat
    15841 tgtggtagat atcgccaacg atccgttaag tacgccgcat atctatttgt gttgcgatcc
    15901 ggtgcgcccg tatgtttctg ccgcgctacc tcatgcggta cgtcgctttg aatttatggt
    15961 gatgccggga gaaaccgaag aacagctgcg tgagccgcaa aatatgcgca agctgttaag
    16021 caaagtgctg cctaatccgg acaatgttga attgattcgc cagcgtgtct acacccacaa
    16081 cgcgcgactg gcgcaacgtt tccgtattga tcgcgtactg ctggcgggcg atgccgcgca
    16141 catcatgccg gtatggcagg ggcagggcta taacagtggt atgcgcgacg cctttaacct
    16201 cgcatggaaa ctggcgttgg ttatccaggg gaaagcccgc gatgcgctgc tcgataccta
    16261 tcaacaagaa cgtcgcgatc acgccaaagc gatgattgac ctgtccgtga cggcgggcaa
    16321 cgtgctggct ccgccgaaac gctggcaggg tacgttacgt gacggcgttt cctggctgct
    16381 gaattatctg ccgccagtaa aacgctactt cctcgaaatg cgcttcaagc cgatgccgca
    16441 atattacggc ggtgcgctgg tgcgtgaggg cgaagcgaag cactctccgg tcggcaagat
    16501 gtttattcag ccgaaagtca cgctggaaaa cggcgacgtg acgctgctcg ataacgcgat
    16561 cggcgcgaac ttcgcggtaa ttggctgggg atgcaatcca ctgtggggga tgagcgacga
    16621 gcaaatccag cagtggcgcg cgttgagcac acgcttcatt caggtggtgc cggaagtgca
    16681 aattcatacc gcacaggata accacgacgg cgtactacgc gtgggggata cgcaaggtcg
    16741 cctgcgtagc tggttcgcgc aacacaatgc ttcgctggtg gtgatgcgcc cggatcgctt
    16801 tgttgccgcc accgccattc cgcaaacact gggcaagacc ctgaataaac tggcgtcggt
    16861 gatgacgctg acccgccctg atgccgacgt ttctgtcgaa aaggtagcct gatatgcacg
    16921 cttatcttca ttgtctttcc cactcgccgc tggtggggta tgtcgacccg gcacaagagg
    16981 tactcgatga ggtcaatggc gtgattgcca gcgcccgcga gcgtattgcg gctttctccc
    17041 ctgaactggt ggtgctgttt gcgccagatc actacaacgg ctttttctat gacgtgatgc
    17101 caccgttctg tttaggcgtt ggagcgacgg caattggtga tttcggcagt gcggcaggag
    17161 agctgcccgt gcctgtggag ctggcggagg cctgtgcgca tgccgtcatg aagagcggga
    17221 tcgatcttgc cgtttcttac tgtatgcagg tggaccacgg gttcgcccag ccgctggagt
    17281 tcctgctcgg tgggctggat aaggtgccag ttctgcctgt gttcatcaac ggtgtcgcca
    17341 cgccgctgcc cggtttccag cgtacccgca tgttgggtga agccattgga cgtttcacca
    17401 gcactctcaa taaacgcgtg ctgttcctag gttccggtgg gctttcccat cagccgccgg
    17461 tgcccgaact ggcgaaagcc gatgcccata tgcgcgaccg cctgttgggg agcgggaaag
    17521 atttacccgc cagtgagcgc gaattgcgtc agcaacgggt gattagcgcc gctgagaagt
    17581 ttgttgagga tcagagaact ctgcatccgc tcaacccgat ttgggataac cagttcatga
    17641 ctttgctgga gcagggacgc atacaggaac tggatgccgt cagtaacgaa gagctttccg
    17701 ccattgccgg aaagtcgaca catgaaatca aaacctgggt cgccgctttt gccgctattt
    17761 ctgcatttgg caactggcgt agcgaagggc gttattaccg cccaatcccg gagtggattg
    17821 ccggatttgg ctcgttaagc gccagaacag agaactgaat atgcaggaga agatgatgag
    17881 ttatcagcca caaaccgaag ccgccaccag ccgttttctg aatgtagaag aagcgggtaa
    17941 aacgctgcgc atccatttta atgactgcgg acaaggcgac gaaaccgttg tcctgctgca
    18001 tggttccggc ccgggtgcta ctggctgggc gaacttcagc cgcaatatcg atccgctggt
    18061 agaggcgggc tatcgggtga tcctgctgga ttgtccgggt tggggcaaga gcgattcgat
    18121 cgttaatagt ggttcgcgat cggatcttaa tgcacgaatc ctgaaaagcg tggtggatca
    18181 actggatatc gccaaaatcc acctgctggg caactcgatg ggtggccata gttctgtggc
    18241 gttcaccctt aactggccgg agcgcgtcgg caaactggtg ctgatgggcg gcggtacggg
    18301 cggcatgagt ttgtttacgc cgatgccaac cgaaggtatt aagcgactga atcagcttta
    18361 tcgtcagccg actatcgaaa acctgaagct gatgatggat atcttcgttt ttgataccag
    18421 cgatttgacc gacgccctgt ttgaagcgcg cctgaataat atgctgtcgc gccgcgatca
    18481 cctggaaaac ttcgttaaga gcctggaagc taatccgaaa cagttcccgg attttggccc
    18541 acgtctggcg gaaatcaaag cgcaaaccct gattgtctgg gggcgcaacg accgctttgt
    18601 gccgatggat gcgggtctgc gtctgctgtc cggcattgcc ggttctgaac tgcatatctt
    18661 ccgcgactgt gggcattggg cgcagtggga acatgccgac gctttcaatc aactggtgct
    18721 gaatttcctc gcacgtgctt aaggaacggt catgacgaag catactcttg agcaactggc
    18781 ggcggattta cgccgcgccg cagagcaggg cgaagcgatt gcgccgttgc gcgatctgat
    18841 tggtatcgat aacgctgaag cggcctacgc cattcagcac ataaatgtgc aatatgacgt
    18901 tgcgcagggg cgtcgcgtgg tggggcgtaa agtgggcctg acacatccga aagtgcagca
    18961 acaactgggc gttgatcaac cggatttcgg gacattattt gccgacatgt gttatggcga
    19021 taacgaaatc attccttttt cccgtgttct gcaaccccgc attgaagcgg agatcgcact
    19081 ggtgttgaac cgcgatttac ccgcaactga tatcaccttc gacgaattgt ataacgccat
    19141 tgaatgggta cttccggcgc tggaggtggt gggtagccgc attcgcgact ggtcgattca
    19201 gtttgtcgat accgtggcag ataacgcttc ctgcggggtg tatgtcatcg gcggtccggc
    19261 gcaacgtccg gcggggttag acctgaaaaa ctgcgccatg aagatgacgc gtaataacga
    19321 agaggtttcc agcgggcgcg gcagcgaatg cctgggacat ccgcttaatg ccgccgtctg
    19381 gctggcacgc aaaatggcca gtctgggtga accgctgcgc gccggagata tcattcttac
    19441 cggggcatta ggtccgatgg tggcagtgaa tgcgggcgat cgttttgaag cccatattga
    19501 aggcataggt tcagttgctg cggcattttc aagcgcagcc ccaaaaggaa gtctgtcatg
    19561 agtaagcgta aagtcgccat tatcggttct ggcaacattg gcaccgatct gatgattaaa
    19621 attttgcgtc acgatcagca tctggagatg gcggtgatgg tcggcattga tcctcagtcc
    19681 gacggtctgg cgcgtgccag acgtatgggc gtcgccacca cccatgaagg ggtgatcggg
    19741 ctgatgaaca tgcctgaatt tgctgatatc gacattgtat ttgatgcgac cagcgccggt
    19801 gctcatgtga aaaacgatgc ggctttgcgc gaagcgaaac cggatattcg cttaattgac
    19861 ctgacgcctg ctgccatcgg cccttactgc gtgccggtgg ttaacctcga ggagaacgtc
    19921 gatcaactga acgtcaacat ggtcacctgc ggcggccagg ccaccattcc aatggtggcg
    19981 gcagtttcac gcgtggtgcg tgttcattac gccgaaatta tcgcttctat cgccagtaaa
    20041 tctgccggac ctggcacgcg tgccaatatc gatgaattta cggaaaccac ttcgcgagcc
    20101 attgaagtgg tgggcggcgc ggcaaaaggg aaggcgatta tcgtgcttaa cccagcagag
    20161 ccaccgttga tgatgcgtga cacggtgtat gtattgagcg acgaagcttc acaagatgat
    20221 atcgaagcct caatcaatga aatggctgag gcggtgcagg cttacgtacc gggatatcgc
    20281 ctgaaacagc gcgtgcagtt tgaagttatc ccgcaggata aaccggttaa tttgccgggc
    20341 gtagggcagt tctccgggct gaaaacggcg gtctggctgg aagtcgaagg cgcagcgcat
    20401 tatctgcctg cctatgcggg caacctcgac attatgactt ccagtgcgct ggcgacagca
    20461 gaaaaaatgg cccagtcact ggcgcgcaag gcaggagaag cggcatgaac ggtaaaaaac
    20521 tttatatctc ggacgtcacg ttgcgtgacg gtatgcacgc cattcgtcat cggtattcgc
    20581 tggaaaacgt tcgccagatt gccaaagcac tggacgatgc ccgcgtggat tcgattgaag
    20641 tggcccacgg cgacggtttg caggggtcca gctttaacta tggtttcggc gcacatagcg
    20701 accttgaatg gattgaagca gcggcggatg tggtgaagca cgccaaaatc gcgacgttgt
    20761 tgctgccagg aatcggcact attcacgatc tgaaaaatgc ctggcaggct ggcgcgcggg
    20821 tggttcgtgt ggctacgcac tgtaccgaag ctgatgtttc cgcccagcat attcagtatg
    20881 cccgcgagct cggaatggac accgttggtt ttctgatgat gagccatatg accacgccgg
    20941 agaatctcgc aaagcaggca aagctgatgg aaggctacgg tgcgacctgt atttatgtgg
    21001 tggattctgg cggtgcgatg aacatgagcg atatccgtga ccgtttccgc gccctgaaag
    21061 cagtgctgaa accagaaacg caaaccggca tgcacgctca ccataacctg agtcttggcg
    21121 tggcgaactc tatcgcggcg gtggaagagg gctgcgaccg aatcgacgcc agcctcgcgg
    21181 gaatgggagc gggcgcaggt aacgcgccgc tggaagtatt tattgccgcc gtggataaac
    21241 tgggttggca gcatggggcc gatctctatg cgttaatgga tgctgctgac gacctggtgc
    21301 gtccgttgca ggatcgaccg gtacgagtcg atcgcgaaac gctggcgctg ggatacgctg
    21361 gtgtttactc cagcttcctg cgtcactgtg aaacggcggc ggcgcgttat ggcttaagcg
    21421 cggtggatat tctcgttgag ctgggcaaac gccggatggt tggcggccag gaggatatga
    21481 tcgtcgacgt ggcgctggat ctgcgcaaca acaaataata ttgactgccg agaacgtgca
    21541 ttttgtatgc ccatcccagc acgactgccg gatgcggcgt gaacgcctta tccggtctac
    21601 atttcgcccg taggcagtca ttaaatagtt ctgattacgc ggctggcaat gtatcagtcg
    21661 cgattcacct gcacacgcaa tgaggttcat cttatgtcga ctcgtacccc ttcatcatct
    21721 tcatcccgcc tgatgctgac catcgggctt tgttttttgg tcgctctgat ggaagggctg
    21781 gatcttcagg cggctggcat tgcggcgggt ggcatcgccc aggctttcgc actcgataaa
    21841 atgcaaatgg gctggatatt cagcgccgga atactcggtt tactacccgg cgcgttggtt
    21901 ggcggaatgc tggcggaccg ttatggtcgc aagcgcattt tgattggctc agttgcgctg
    21961 tttggtttgt tctcgctggc aacggcgatt gcctgggatt tcccctcact ggtctttgcg
    22021 cggctgatga ccggtgtcgg gctgggggcg gcgttgccga atcttatcgc cctgacgtct
    22081 gaagccgcgg gtccacgttt tcgtgggacg gcagtgagcc tgatgtattg cggtgttccc
    22141 attggcgcgg cgctggcggc gacactgggt ttcgcggggg caaacttagc atggcaaacg
    22201 gtgttttggg taggtggtgt ggtgccgttg attctggtgc cgctattaat gcgctggctg
    22261 ccggagtcgg cggttttcgc tggcgaaaaa caggctgcgc caccactgcg tgcgttattt
    22321 gcgccagaaa cggcaaccgc gacgctgctg ctgtggctgt gttatttctt cactctgctg
    22381 gtggtttaca tgttgatcaa ctggctaccg ctgcttttgg tggagcaagg attccagcca
    22441 tcgcaggcgg caggggtgat gtttgcactg caaatgggtg cggcgagcgg gacgttaatg
    22501 ttgggcgcat tgatggataa gctgcgtcca gtaaccatgt cgctactgat ttatagcggc
    22561 atgttagctt cgctgctggc gcttggaacg gtgtcgtcat ttaacggtat gttgctggcg
    22621 ggatttgtcg cggggttgtt tgcgacaggt gggcaaagcg ttttgtatgc cctggcaccg
    22681 ttgttttaca gttcgcagat ccgcgcaaca ggtgtgggaa cagccgtggc ggtagggcgt
    22741 ctgggggcta tgagcggtcc gttactggcc gggaaaatgc tggcattagg cactggcacg
    22801 gtcggcgtaa tggccgcttc tgcaccgggt attcttgttg ctgggttggc ggtgtttatt
    22861 ttgatgagtc ggagattaag aatgcagccg tgtgccgatg cctgacgcgc cttattaagc
    22921 aggggcgaaa gggacttgcc cccaatacac ccacggcgtt tacaggtata ctcgctaaaa
    22981 attattcagc gggtttggaa acaaagatgg caaaacttac cttacaagag cagttgctca
    23041 aagcaggatt agtcaccagc aaaaaagcgg cgaaggtcga gagaacggcg aaaaaatcgc
    23101 gcgttcaggc gcgtgaagct cgggcggcgg tagaagaaaa taaaaaggca cagcttgagc
    23161 gtgataaaca gcttagcgaa cagcaaaaac aagcggcgtt ggcgaaagaa tataaagctc
    23221 aggtgaagca gcttattgaa atgaaccgga tcaccattgc caatggcgat attggtttta
    23281 acttcactga tggcaatctg attaagaaga tttttgtcga taagctcaca caggcgcagt
    23341 tgattaatgg tcgtctggcg attgcccgct tgttggtcga taacaatagc gaaggtgaat
    23401 acgccattat tcccgccagc gttgccgata aaattgctca gcgtgatgcc agcagtattg
    23461 tgttgcacag cgcgctcagt gccgaagagc aggatgaaga cgacccgtat gccgacttca
    23521 aagtgcctga tgatttgatg tggtaaacat agataagtcg tgcgcagatg cctgatgcga
    23581 cgctatgcgc gtcttatcat gcctacggcc tgcgggcgaa atgtaggcgg ataaggcgtt
    23641 cacgccgcat ccggcagtcg tgcactatta tcaacgcata ttcagtttat tggcgtggta
    23701 ggcaatatgc tcgccaataa aactggagac aaaataatag ctgtggtcgt aaccctcgtg
    23761 ataacggatt aacgttttga tattcatctc ctggcagatc ttttcaagat tttgagtccg
    23821 cagctgttcc gcgtaaaaat catcactcaa cccctgatca accatgattt ccgcaacgcg
    23881 ttgaccttgt gaaataagac tcaccggatg gtaatccaac caggcctctt tattttcacc
    23941 aagatatgcg gcaaaggctt gctgtcccca cggtacttgc gatggggaga caatgggcga
    24001 aaacgccgag acgctgacat attcatctgg gttacgtaac gccagcacca gcgcgcccag
    24061 tccgcccata gaatgaccag agatagactt tttggccgtt gccggaaaat gattcatcac
    24121 taaatccggc agctcgttgc ggatatagtc atacattttg taatgttcat tccacggcgc
    24181 ttgcgtcgcg ttcaggtaaa acccggcacc ttgcccgaga tcgtaacggt cggcatctgc
    24241 gacatgactg cctcgcggac tggtgtccgg cgcaacaaca ataatgttgt gctcagccgc
    24301 gtaacgctgc acccccgatt tagtaatgaa attctgctca ttgcaggtca ggcccgaaag
    24361 ccagtacagc accggtaatt tttcacctgc agcttttggt gggagataga cgccgacatt
    24421 catttcacat ttcagtgatg cggaataatg ccgatacaca ttttgccagc cgccaaagct
    24481 ggcatgtttt tcaatgagtt ccatcagtca ttatctcacg gttaatcccc cggacggctg
    24541 cccggagaga gtattaagaa ccccgtctga agtggacggg gtaaaccagc gggaaatcag
    24601 taacgaatta cggttcgaat ggatttgcct tcatgcatca ggtcgaaggc gtcattaatt
    24661 tcatccaggc tcatggtatg cgtgacaaac ggttccagat cgatatcacc tttcatcgca
    24721 tcttcaacca tacccggtaa ctgggaacga cctttcacgc cgccaaacgc ggaacctttc
    24781 catacgcgac cagtgaccaa ctggaatgga cgggtggaga tttcctgacc ggaacccgcg
    24841 accccgatga tcaccgactg accccagccg cggtgcgcac tttccagcgc cgcacgcatc
    24901 acgttgacat taccgatgca ttcaaaggta tggtcgatac cccatttgtt gatatccagc
    24961 aggacatctt taatcggttt gtcgtagtca ttcgggttaa tgcagtcggt ggcaccgaag
    25021 cgacgcgcca gatcgaattt cttcgggttg gtatcgatag cgataatccg tcccgctttc
    25081 gcctgacgcg cgccctgaac cactgccaga ccaatcgcgc caagaccaaa cacagcaaca
    25141 gaatcccctg gctggacttt agctgtgttg tgtaccgcgc caataccggt ggtcacgcca
    25201 cagcccagca ggcagacgtg ttcatggttt gcttctggat taatttttgc cagagacact
    25261 tccgcgacta cggtgtattc actgaatgta gaacacccca tgtagtgata aagcggctgc
    25321 ccattgtaag aaaaacgggt ggtgccgtct ggcatcaggc ctttaccctg ggtttcgcga
    25381 accgcaacac agaggttagt tttgccagaa cgacagaact cacactcgcc gcactctgcg
    25441 gtgtaaagcg ggatcacatg gtcgcctggt ttgacgctgg ttacgccttc accgacttca
    25501 accacaacgc cagccccttc gtgaccgaga accaccggga atacaccttc cgggtcatcg
    25561 ccggagaggg taaatgcgtc ggtatggcaa acgccggtat gggtgacttt aatcagcact
    25621 tcaccttttt tcggtggtgc aacgtcaatt tcaacgattt ccagcggttt accgggagca
    25681 aatgcaacgg cagcacgtga tttcatctct cgctcttcct caatatggta atagattcag
    25741 ctatttaaga taggcacgaa ccagttcaat agtgtcgtca acggattggc tgacttcgcg
    25801 gctgtagcag tcatttcggt caaacgtttc ccggatatgg ctttcaagca cttctgccat
    25861 cagcccatta gccgcgcccc gaacggcagc gatctgttgg agtatggcac ggcattcggc
    25921 atcaccctcc agcgaccgtt ccagagcatc aatctgccct cgaatacgac gaactcgagt
    25981 aaggaccttt ttcttctctt ccggagtact gggcatttcg cacctcatca tctgcacgca
    26041 atatactata ggggggtatg ctatatgtca atgcataccc ccctatagta tataacaatg
    26101 gagaataatt gttgtccgaa gcctaaccag gtgaacggtc cctcaagaca gatggggacc
    26161 gtttatgtgc tatttttatg ccgcatccgc taaaacaaac atcccgtaag gatggatttc
    26221 gaggtaatag tgctcaccaa tatcgggctg taaacgggtg gcgtttactt gtaataaaat
    26281 ctcctgtcca tgccactcta cggtcacttc gtactgcggc cccatgtagg cgacatggcg
    26341 aataacacaa cgctgactct cttcgccgtg attgcttaag gtgatggctt ccgggcgaac
    26401 gccgacggtg ccgctacctt gcaccgggaa atgcgccgcg cgcggcaggc gataaccgta
    26461 gatatcgaca tactcttcgc taaagttcgc cgggaacagg ttggcgtcgc ccataaaact
    26521 tgccataaag cgggaggctg gctgacgata gagatcctgc ggtgagccaa tctgcatgat
    26581 gtgaccttta ttcatcacca gcacagtgtc ggaaaccgcg aaggcctcac tttgatcgtg
    26641 agtcacgtag agcgacgtga tattaaactg cttttgcagc tcgcggatct tatcgcgcat
    26701 gctgcggcgc aggttggcat cgaggttact taacggctca tcaaacagca gcacctttgg
    26761 tttgaggatc aacgcgcggg ccagcgccac gcgctgttgc tgaccaccgg aaatctggtc
    26821 gacatagcgg tcctcgaacc cttccagatc caccattgcc agcgcctctt tcacccgctg
    26881 ctttacttcg ctacgcgaca cgccgagcat ctttaacccg tagccgacgt tttcgcccag
    26941 cgacatatgc gggaacagag cgtatgactg aaacaccatg cagatatcgc gctgctgaat
    27001 ggaacgatgc gtgacatctt cgccatcaat aaagatttgc ccttcactcg gtttttccag
    27061 cccggcaacc agacgcaaaa tggtggtttt gccgcagccg gaagggccaa gcagcgtcac
    27121 catttgccct tgtgggatgg tgagattgat attgtcgata accgtattac tgccgaatcg
    27181 tttagtgacg ttgcgcagtt ccacaaaatt tttctgactc atagtgcgct ccattacgcc
    27241 tggtttttgg ctttagaacg ggagatacgt gcttctccga tcagccagtc aaagaggaaa
    27301 ataatcgcca gcatcaccac gatcagaatc gaaccatagg cgatggctac gccatattcg
    27361 ccgtcttcaa cgcggttaag aatgtaggcg gttgccacgc gggtgtccgg cgtgacgaga
    27421 aagacgatgg cgctgaccgt cgtgatggcc cgcacaaagc tgtagatcag ggccgagaga
    27481 atggccggac gcagcagcgg cagtaaaatg tgggttatgg ttcgcaagga acccgcgcgc
    27541 aggctgagtg aggcttcatc cagcgatttg tcgatctgcc ccaggcctgc aatcccggcg
    27601 cgaataccca ccggaacgtt acgcatcacc atcgagataa tcacaattgc cgccgttccg
    27661 gtgagataaa ccggagcgct gttaaaggca agaatatacg aaacgcccgc caccgtaccc
    27721 ggtacggcaa agcacagcat ggtggtgaac tcgatggtct ttttgccttt gaattgttga
    27781 cgcaccacca cccaggcgat gagcaggcca aagatagcgg taatgggagc ggcaatcccg
    27841 gcgtagagca gggtatccag cagtgaaggc cacgcgccgt cgctcattcc ctgaccaaat
    27901 agcttgataa agttatccag cgtcagggtg tagtccacgc cccagttcac cgtgaagctg
    27961 ccgtagaaaa tgctgccgta gagcagggcg ttaaaggcaa tccatatcgc aagtatggcg
    28021 accacgctcc acaccagggt gaccggaagc ggctggacgt cgccacggta ggatttgccg
    28081 gaaaccgtga cgtaagagcg tttaccaatc cacatatact gtatgcagaa caccagcagc
    28141 gagaacagca gcaggaaagc gccaagggtg ctggcggcct gataatccag ctgcgagccg
    28201 gtaatataga agtaaatttg cgtcgccagt acgtcgaagt taccgccaag taccagcggg
    28261 ttactgaaat cagccagtga ctgtaccacg acgattaaaa aggcattggc gagcgcgggc
    28321 ttcaacaacg gcacaaacac gccgttaaag gtttgccagc ggctggcgcg cagggtgtag
    28381 gaggcctctt ccagcgaggg atgaatggtt ttaatggccc cgtcgaggat cataaatgcc
    28441 attggcgtga atgccagaac ctgtgccagc cagatcccgg taaaaccgta cagccagttg
    28501 gtgttggtca gcccaaacca ctccaccata aattccgtaa catagccgga acgccccatc
    28561 atgagcgtca cgcctaagcc gaccacaaac ggtggcgtaa cgatgggcaa aatggaaaag
    28621 atgcgaccaa taatggcgct gcgtttagcg atgcgcgtgg tataaatcgc cagcaccagg
    28681 ccgaagaaag cacagcctat ccccaccgct atcgacaaga cgatagagtt gacgatcacc
    28741 tgaacaatat gtgcctgcga gagcacggtc ataaacgcca gcggggcgag ctctcctgcg
    28801 tcgttggtga acatcgggat aaaaatggcg atgctcggcc agacgataaa aatgccaatc
    28861 agcgccacaa tcgcaatcag tgagccgatc acgaagcggt cgccgcccag ccattcgagg
    28921 cgggttaagg ccagcgtcat aatcgcgcct agttcgacaa acagcacgat ggcggcgtag
    28981 cctaaaccgc gtccagccac ggtggcgctg atgacgataa acgccataca gagcaatgcc
    29041 cagccggcat cgaaataatg acgactacgc tgttcgcgcg tggcctgacg aaacgggcgg
    29101 accagcaaca ggctcgggag taaataccac agccagctga tattaaaatg cgaccagccg
    29161 tacgccccga ggatctcatc actcgtggat tccagcaggc cataatcgag gctccagctc
    29221 ggcaacagga caaatgccag ccagcccagc aaaacccaaa ggaaaatcgc atcccgcttt
    29281 ttcacgggat ggagtgcaag tgtctgtgac atagcagttc cggtatggtt ggccggggca
    29341 ggcgtctgcg ctgcacccgg caaatgcgcc taatgcgggt ttatttaccc atcttcactt
    29401 cgctgaccca tttattaatc aatgccttac gaacatccgt ggaaccatat ttatccatgt
    29461 cgtagttgat taatttcagg tcgtcgagct tcagcgaatt tggcgacgtt tcggcggtgg
    29521 tattagtcag gatctgataa gacttccctt tcttccacgc cagttcctga gcttcttttg
    29581 ataataccca gtccacaaac agtttggcgt tgtcgaggtt gcgcgcgcct ttcagaatgc
    29641 tgacgccgcc aatttcgtag cccgtacctt cacacgggga aatcaactcc agcggcgcgc
    29701 cctgttcttt ttccagcgag tagtcatgca ggaagccaat accaatcgcc gtttcgccac
    29761 gggcggcgtt acgtgccggg gctatgcctg atttggtgta ctgagagacg ttggcgttca
    29821 gttgttttag ataatcaaag gcctgatctt ccccccacag ctgcgcaaaa gtagccaggg
    29881 cggtgtaggc agtaccggaa ctttgcggat cggcaatttg aatttcgcct ttatattccg
    29941 gtttggtgag atctttccag cacttcggca ccggcaggtt tttctctttc agacgatcgg
    30001 tgtttacgcc aaaaccgagg atccccacat aaacggcaga ggacagattc cctttcactt
    30061 ttgccggatc gcggaatttc tccatgatct gctcaaggtt aggggattta tacgcctgta
    30121 gcaggcccat ctcgccagcc tgcgattgcg ggtctaaagt gccgccgtac cagacgtcag
    30181 cctgggggtt tttcttttca gcatcgactt ttgccagggt gcttccggaa ccgttgcgga
    30241 taaacgaggt ttttacatca tatttctcgc caaacgcttt ggtttcggcc tcacacattt
    30301 cattggtggc gctgcaatag accaccagcc ggccttttgc ctgcgcggca cccgttaaag
    30361 tcgccagcgc aacggcagaa gcaatcagag tagaggtaaa ggtcattttc attgttttat
    30421 ccttcgagcg tgagtggtgg tgatgcccgc catcagtaac ggcatcagca gtaaacccat
    30481 aagtactgcg gcgaccgaga gtaaactgaa cattcccggc cagccgtagc gttcgatgac
    30541 cagcgacagc ggccagcctg ccagcgccgc gccaaggtaa gcgaacaggc cgagaaatcc
    30601 ggtgattgaa cccgccgcag ctttgtgccc acactccacc gccgcaagtc caatcagcat
    30661 ttgtgggcca aagacgaaaa agcccacggt aaaaaagcac actgccagca gagcgtagtg
    30721 atgaaccggt gccagccaca gtgcggcgac tgacaccatc aaccccaacg tgaacagcaa
    30781 aatcattggc gctcgttgcc cgctaaacag caaatccgat ccccatccgg caaataacgc
    30841 gccgagtaag ccgcccactt caaacagcat taccgtggcg ttggcgctga gcaggttgac
    30901 accgtggctt tccgtcagcc agatattgcc ccagtcgtta agcgcaatcc ggatcacata
    30961 aaccagtaca tatgagacgc ccagtagcca gatcagcgga ttttgcagca tggtggtgcg
    31021 caacatttgc cacagcccca tcggcggact ttgttgttcc tgacgtaact ccagcggatc
    31081 gtgacgccag tgaccaactg tcggtaatcc ctcttcctgg ggcgtgcctt tgagttgtag
    31141 cgttaaccag atccctaatg ccatgctgat tatccccggt gtcagcatcg cggcctgcca
    31201 gccccaccag tgggcggcaa aggcgctaat cagcggaata atcgctccgc caatgttgat
    31261 cgacatattc cagcagcccc accagaaacc gcgctcattg cgtgagtacc agtgggtcag
    31321 caagcgggca cacggtggcc atccccagcc ctgaaaaaaa ccgttcagtg tccagaccac
    31381 aagcagaagc gtaagtgatt cgccaaaggc aaacaccacg ttcaataacc cggtggcgaa
    31441 caagccgacg cccataaaac cgcgttgtcc gtggctgtca tgccacagtc cggcggtaaa
    31501 tttcgacaat ccgtagctga gataaaacag cgaacccagc aggccaatgt cgcctttatc
    31561 cagtccgagg tcggtttgta gcgccggaag gacgtaattc acgctttttc gcgtcagata
    31621 aaatgcggcg tagccaataa tcatgcacat cagcaatcgc ggacgtaatg tccggtagca
    31681 ttgattaatt tctctcgctg aacgcgcgtg catggttgtc tccatgttgc tgactatatg
    31741 aaaatgaaag tgaaagggga tgagagaaaa tccgggatac ctaagacttt ttcctggtta
    31801 tccggcgatt tgttgcaaaa atgtgggcaa gttaacaatt acccgcgtgc cgtgctgttt
    31861 ttcgagcgtt aagtcgccgc ccagggcgct gacgcgctca cgcatcccct gaatgccaaa
    31921 tccagccatt ttcccgctgg cgatccccac accgttgtcg ctgacttcca gatgcaatac
    31981 ctctccctgc tggcgcagga taatcgtcac ttcgctggct tcggcgtgtt tacagatgtt
    32041 attgagcaac tcctgcaata accgatacaa ggtaaagcgc accgtttcgt tttctggtgt
    32101 ggagttcagt tgataggcaa actggcaatg aatgccgcgt tcactaaagg caaattcatt
    32161 aatcagatgc agcaacgctt cgcgaaatgt cagttcatca agcgcgggag gccgcaactg
    32221 gcgcagcaat tggcgggtgg agagatgaat gcggcgggcg agctcattga tttggctggc
    32281 tgcggattga atttgcgccg gatcacgcgc ccgcttcacc agttgcgact ggatctgaat
    32341 ggcggtgatg ttctgaccga tttcgtcgtg cagctctcgc gccaggtttt ttcgcgtgtc
    32401 ctcctcggtg tggatcagct tttccgccaa tgcccgccgc gctgccagct cggcctccag
    32461 ccgttgtcgg taatggtgca ggtttagcgc cagatgttgc tggcgactga tggcaatgcc
    32521 caggccaatt cccagcaagg cctgggtcgc gaggaaaatc tccagctcca ccaggttact
    32581 aaagcccacg ccaatctggc gggcgatggt gatcatcatg ctgcccaaaa gcccggagag
    32641 tacgccacct tgccagccaa atttccacgc catcaccaca ttgggcaaaa agaccacgat
    32701 caacagcaga cgttcaattt caggtgacag caccatctga gtgccgatac caataataaa
    32761 aaacaggcta caccagatga ttaaagaggt acgcaagggg ggattatgcg tatcgagtcc
    32821 caggagctgg tagcgatgct gctggcgcag aaactcaaag ataagataga caaacggcgt
    32881 cagtaatacg ccgccggtaa atgaggtcaa acccggtagc attattgggc ctgacataaa
    32941 aggggagagc agggcggtat tcaacaatga agctgccgtg acagtggcta acagcaacgt
    33001 cagacgctgc cagtaaagtg gaaaacggtg ccagaagttt tgcgcaagga gggcaggaaa
    33061 caggctaata accggcgttg ccagcatgag ataaccgttc aatagctgtt cactatggag
    33121 ccagaacatc atcagcaaag gggggagcac caacgcaggc cagtagcggc gagagagcaa
    33181 aataacaagg gcgagataaa ctccctgcgg gagaaacaac accgcctgtt gaccgttatt
    33241 ggtcagataa aagctgagcg tccacagcat cagccagccg gttccccagg ctagcacaat
    33301 aaacagcaaa atcacccaat gacgaagatt gcgcgacatt aatgccccgc cagcagatgg
    33361 tggtcgagcg caaaatgtac cagttcaatg gtgctatggc agttcagttt tcccagcaca
    33421 ttggcgcgat gcacatgtac cgttttatga ctgagttcga gtttaaaggc gatctcttta
    33481 acgctgtctc ctttgaccag taactcaaac acttcacgtt ctcgtggggt gagaatttcc
    33541 agcgcctggg cgggttgccc gccgccgcgt aaagcccgaa tcgcatcggc acagagataa
    33601 tgcccgccca gcccgaccga gcgcaccgcc tgtactaact cttccggccc acagcgtttg
    33661 gttaaatacc cgctggcacc ggcatccagc gcgctttgca caaagattgg cgcgtcataa
    33721 atgctaagaa taatggcgcg aaattgcggt ttttgtgcac gcaggcgctt aagcaaactg
    33781 aggccgtttt cgtccggcat ggcaatatca atgacggcaa cattgacgtc atcacggatt
    33841 aatgccgacc atgcctgcgc ggcactactg tactgaccaa tgacctccag atcgtcttcc
    33901 agagagagta attgtgcgaa gccagagcgc accacaacat ggtcatccac cagcactacc
    33961 cgaatcatac ttttcttctc ttattatcta attctcctat catgcatcac ggcgcggcgc
    34021 tggcaatcaa ccagcgacag atatggaatg gatgtaacaa aatggcaata atttgccgga
    34081 gcgcgcacat cttatctggc ttgtgaaccg gcgggatacg ttcgccaaaa ccgcaaaatg
    34141 cgcaaactcg ctaaccattg ccgatttttt tctaaaaacg ctttgttctt aacaagacct
    34201 tttaattcct ttatcaaact gacccgcaac gaaatactgc tcccataaca agacagggga
    34261 gcagacaatc atggcaattt catcgcgtaa cactcttctt gccgcactgg cattcatcgc
    34321 ttttcaggcg caggcggtga acgtcaccgt ggcgtatcaa acctcagccg aaccggcgaa
    34381 agtggctcag gccgataaca cctttgctaa agaaagtgga gcaactgtgg actggcgcaa
    34441 gtttgacagc ggagccagca tcgtgcgggc gctggcttct ggcgacgtgc aaatcggcaa
    34501 ccttggttcc agcccgttag cggttgcagc cagccaacag gtgccgattg aagtcttctt
    34561 gctggcgtca aaactgggta actccgaagc gctggtggta aagaaaacta tcagcaaacc
    34621 ggaagatctg attggcaaac gcatcgccgt accgtttatc tccaccaccc actacagcct
    34681 gctggcggcg ctgaaacact ggggcattaa acccgggcaa gtggagattg tgaacctgca
    34741 gccgcccgcg attatcgctg cctggcagcg gggagatatt gatggtgctt atgtctgggc
    34801 accggcggtt aacgccctgg aaaaagacgg caaggtgttg accgattctg aacaggtcgg
    34861 gcagtggggc gcgccaacgc tggacgtttg ggtggtgcgc aaagattttg ccgagaaaca
    34921 tcctgaggtc gtgaaagcgt tcgctaaaag cgccatcgat gctcagcaac cgtacattgc
    34981 taacccagac gtgtggctga aacagccgga aaacatcagc aaactggcgc gtttaagcgg
    35041 cgtgcctgaa ggtgacgttc cggggctggt gaaggggaat acctatctga cgccgcagca
    35101 acaaacggca gaactgaccg gaccggtgaa caaagcgatc atcgacaccg cgcagttttt
    35161 gaaagagcag ggcaaggtcc cggctgtagc gaatgattac agccagtacg ttacctcgcg
    35221 cttcgtgcaa taaaaggagg cgcagatgct gcaaatctct catctttacg ccgattatgg
    35281 cggcaaaccg gtactggaag atatcaacct gacgctggaa agcggcgagc tactggtggt
    35341 gctggggccg tccggctgcg gtaaaaccac cctgctgaat ttgattgccg gttttgtgcc
    35401 ttatcagcat ggcagcattc aactggcggg taagcgtatt gaggggccgg gagcagagcg
    35461 tggcgtagtt tttcagaatg aagggctact accgtggcgc aatgtacagg acaacgtggc
    35521 gttcggcctg caattggcag gtatagagaa aatacagcga ctggaaatcg cgcaccagat
    35581 gctgaaaaaa gtggggctgg aaggcgcaga aaaacgctac atctggcagc tttccggtgg
    35641 tcaacgtcag cgggtgggga ttgcccgcgc gctggcggcg aatccccagc tgttattact
    35701 cgacgaaccg tttggtgcgc tggacgcctt cacccgcgac cagatgcaaa ccctgctgct
    35761 gaaactctgg caggagacgg gcaagcaggt gctgttgatt acccacgata tagaagaagc
    35821 ggtgtttatg gcgactgaac tggttctgct ttcacccggc cctggccgcg tgctggagcg
    35881 gctgccgctc aactttgctc gccgctttgt tgcgggagag tcgagccgca gcatcaagtc
    35941 cgatccacaa ttcatcgcca tgcgcgaata tgttttaagc cgcgtatttg agcaacggga
    36001 ggcgttctca tgagtgtgct cattaatgaa aaactgcatt cgcggcggct gaaatggcgc
    36061 tggccgctct cgcgtcaggt gaccttaagc attggcacgt tagcggtttt actcactgta
    36121 tggtggacgg tggcggcgct gcaactgatt agcccgctgt ttttgccgcc gccgcaacaa
    36181 gtactggcaa aactgctcac cattgccgga ccgcagggat ttatggacgc cacgctgtgg
    36241 cagcatctgg cagccagcct gacgcgcatt gtgctggcgc tactcgcggc ggtgttgatc
    36301 ggcattccgg tcgggatcgc catggggcta agcccgacgg tgcgcggcat tctggatccg
    36361 ataatcgagc tttatcgtcc ggtgccgccg ctggcttatt tgccgctgat ggtgatctgg
    36421 tttggtattg gtgaaacctc gaagatctta ctgatctatt tagcgatttt tgcaccggtg
    36481 gcgatgtcgg cgctggcggg ggtgaaaagc gcgcagcagg ttcgcattcg tgctgcccag
    36541 tcgctgggtg ccagccgtgc gcaggtgctg tggtttgtca ttttgcccgg cgcgctgccg
    36601 gaaatcctca ccggattacg tattggtctg ggtgtgggct ggtctacgct ggtggcggcg
    36661 gagctgattg ccgcgacgcg cggtttagga tttatggttc agtcagcggg tgaatttctc
    36721 gcgactgacg tggtgctggc ggggatcgcg gtgattgcga ttatcgcctt tcttttagaa
    36781 ctgggtctgc gcgcgttaca gcgccgcctg acgccctggc atggagaagt acaatgagtg
    36841 aacgtctgag cattaccccg ctggggccgt atatcggagc acaaatttcg ggtgccgacc
    36901 tgacgcgccc gttaagcgat aatcagtttg aacaacttta ccatgcggta ctgcgccatc
    36961 aggtggtgtt tctgcgcgat caagccatta cgccgcagca gcaacgcgcg ctggcccagc
    37021 gttttggcga actgcatatt caccctgttt accctcatgc cgaaggggtt gacgagatca
    37081 tcgtgctgga tacccataac gataatccgc cagataacga caactggcat accgatgtga
    37141 catttattga aacgccaccc gcaggggcga ttctggcagc gaaagagtta ccttcgaccg
    37201 gcggcgatac gctctggacc agcggtattg cggcctatga ggcgctctct gttcccttcc
    37261 gccagctgct gagcgggctg cgtgcggagc atgattttcg taaatcgttc ccggaataca
    37321 aataccgcaa aaccgaagag gaacatcaac gctggcgcga ggcggttgcg aaaaatccgc
    37381 cgttgctaca tccggtggtg cgaacgcatc cggtgagcgg taaacaggcg ctgtttgtga
    37441 atgaaggctt tactacgcga attgttgatg tcagcgagaa agagagcgaa gccttgttag
    37501 gctttttatt cgcccatatc actaaaccgg agtttcaggt gcgctggcgc tggcaaccga
    37561 atgatattgc gatttgggat aaccgtgtga ctcagcacta tgccaatgcc gattacctgc
    37621 cacagcggcg gattatgcat cgggcgacga ttcttgggga taaaccgttt tatcgggcgg
    37681 ggtaatacga gagtggacgg tcctctcgcc cccttgggga gagggttagg gtgagggggc
    37741 gttcaccgtg ctttcaacag gttaactccc cctttctgag aggaaacaaa attaacgcag
    37801 aatcttcttc tcagccaaat ccagcgcaaa gtagctgaaa atcagatccg cacccgcacg
    37861 cttaatcgaa cctaagcttt cgagcacgac tttctcttca tctatagcac ccgccagcgc
    37921 ggcgaactta atcatcgcat actcaccgct cacctgatac gcgccaatcg gcaattcagt
    37981 acgttcacgc agctcacgca cgatgtcgag gtacgctcca gcaggtttaa ccatcaggca
    38041 gtctgcgccc tgggcttcat ccagcagtga ctcacgaatc gcctcacgac ggttcattgg
    38101 gttcatctga tagcttttgc ggtcgccttt taatgcgctt ccggcagctt cacggaacgg
    38161 accataaaag gaggaggcga acttggtcga atacgacata atcgccgtat ctttaaagcc
    38221 agcagcgtcc agcgcctggc gaatcgcctg tacctggcca tccatcgcgg cggaaggggc
    38281 gatgaagtct gcacctgcag cagctgcaac cacggcttgc ttgcctaaat tttccagagt
    38341 cgcgtcgttg tcgacgccat gctcgcacag cacaccgcag tgaccgtgag aagtgtattc
    38401 acagaagcag gtgtctgaca tgacaatcat ttctggcacc gtctgcttgc agatgcgcga
    38461 catacgcgct accagtccat cttcccgcca ggcatcgctg ccggtttcat cggtatggtg
    38521 agagatgccg aaagtcatca cggaacgaat accggcgttg gcgatgcgtt caatttcgcg
    38581 tgccagatgt ttctctggaa tgcgcatcac gccaggcatg gcttcaacgg ctttgtagtc
    38641 gtcaatttct tcttcaacaa agatcggcaa caccaggtcg ttaaggctaa gtgttgtctc
    38701 ttcaaacata gcgcgcagcg caggagattt gcgcaggcga cgagggcgtt ggattaagtc
    38761 tgtcatggtc tgcctgatgt ttgtggaatt aaggtgcaca gtatacctga agcggggctg
    38821 gagtgtttta cgaaagttgt cacgatgttg acaagaagag aatgaaagag agaccagggc
    38881 atctttgtaa tagaaatagc aataacttca tattcactga aaatattttt taatttttat
    38941 ttataggtgc tggttattat ttttgtcaat ttggttgaac tgtcaaaaat tgaattactt
    39001 aaagttcatt gcttattgat aaattatttg tctga
//
LOCUS       DBFIFM010000016        38882 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.016, whole genome shotgun sequence.
ACCESSION   DBFIFM010000016 DBFIFM010000000
VERSION     DBFIFM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.016"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(309..944)
                     /gene="nth"
                     /locus_tag="N7C74_000789"
     CDS             complement(309..944)
                     /gene="nth"
                     /locus_tag="N7C74_000789"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HGU2430723.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNS
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(944..1639)
                     /gene="rsxE"
                     /locus_tag="N7C74_000790"
     CDS             complement(944..1639)
                     /gene="rsxE"
                     /locus_tag="N7C74_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HGU2430724.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(1643..2263)
                     /gene="rsxG"
                     /locus_tag="N7C74_000791"
     CDS             complement(1643..2263)
                     /gene="rsxG"
                     /locus_tag="N7C74_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HGU2430725.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGNFDQFTGATITPRAVVNAVKRAGLYAQTLPEQLSQLPACGE"
     gene            complement(2267..3325)
                     /gene="rsxD"
                     /locus_tag="N7C74_000792"
     CDS             complement(2267..3325)
                     /gene="rsxD"
                     /locus_tag="N7C74_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HGU2430726.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(3326..5644)
                     /gene="rsxC"
                     /locus_tag="N7C74_000793"
     CDS             complement(3326..5644)
                     /gene="rsxC"
                     /locus_tag="N7C74_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HGU2430727.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRRREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISMRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVTDPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEPVDPRKAAVEAAIARAKARKLEQQQANAEPEEPVDP
                     RKAAVEAAITRAKARKLEQQQANAEPEEQVDPRKAAVAAAIARAKARKLEQQQANAEP
                     EEQVDPRKAAVAAAIARVQAKKAVQQKVVNED"
     gene            complement(5637..6215)
                     /gene="rsxB"
                     /locus_tag="N7C74_000794"
     CDS             complement(5637..6215)
                     /gene="rsxB"
                     /locus_tag="N7C74_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HGU2430728.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(6215..6796)
                     /gene="rsxA"
                     /locus_tag="N7C74_000795"
     CDS             complement(6215..6796)
                     /gene="rsxA"
                     /locus_tag="N7C74_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HGU2430729.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(6873..7313)
                     /gene="ydgK"
                     /locus_tag="N7C74_000796"
     CDS             complement(6873..7313)
                     /gene="ydgK"
                     /locus_tag="N7C74_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HGU2430730.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGAQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(7399..7614)
                     /gene="ydgT"
                     /locus_tag="N7C74_000797"
     CDS             complement(7399..7614)
                     /gene="ydgT"
                     /locus_tag="N7C74_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HGU2430731.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(7887..8012)
                     /gene="blr"
                     /locus_tag="N7C74_000798"
     CDS             complement(7887..8012)
                     /gene="blr"
                     /locus_tag="N7C74_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HGU2430732.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            8255..9295
                     /gene="ydgJ"
                     /locus_tag="N7C74_000799"
     CDS             8255..9295
                     /gene="ydgJ"
                     /locus_tag="N7C74_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HGU2430733.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAIMLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(9331..10332)
                     /gene="add"
                     /locus_tag="N7C74_000800"
     CDS             complement(9331..10332)
                     /gene="add"
                     /locus_tag="N7C74_000800"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HGU2430734.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLNAEEKRALREKVAAK"
     gene            complement(10436..11608)
                     /gene="malY"
                     /locus_tag="N7C74_000801"
     CDS             complement(10436..11608)
                     /gene="malY"
                     /locus_tag="N7C74_000801"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HGU2430735.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(11618..13210)
                     /gene="malX"
                     /locus_tag="N7C74_000802"
     CDS             complement(11618..13210)
                     /gene="malX"
                     /locus_tag="N7C74_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HGU2430736.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLLPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVVAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            13385..14413
                     /gene="malI"
                     /locus_tag="N7C74_000803"
     CDS             13385..14413
                     /gene="malI"
                     /locus_tag="N7C74_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HGU2430737.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLVHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            14525..15292
                     /gene="hdhA"
                     /locus_tag="N7C74_000804"
     CDS             14525..15292
                     /gene="hdhA"
                     /locus_tag="N7C74_000804"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HGU2430738.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            15521..16111
                     /gene="uidR"
                     /locus_tag="N7C74_000805"
     CDS             15521..16111
                     /gene="uidR"
                     /locus_tag="N7C74_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HGU2430739.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            16502..18315
                     /gene="uidA"
                     /locus_tag="N7C74_000806"
                     /pseudo
     CDS             16502..18315
                     /gene="uidA"
                     /locus_tag="N7C74_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
     gene            18312..19685
                     /gene="uidB"
                     /locus_tag="N7C74_000807"
     CDS             18312..19685
                     /gene="uidB"
                     /locus_tag="N7C74_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HGU2430740.1"
                     /translation="MNQQLSWRTIVGYSLGDIANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            19724..20988
                     /gene="uidC"
                     /locus_tag="N7C74_000808"
                     /pseudo
     CDS             19724..20988
                     /gene="uidC"
                     /locus_tag="N7C74_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
     gene            complement(21033..22541)
                     /gene="ydgA"
                     /locus_tag="N7C74_000809"
     CDS             complement(21033..22541)
                     /gene="ydgA"
                     /locus_tag="N7C74_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HGU2430741.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLVKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(22642..23817)
                     /gene="manA"
                     /locus_tag="N7C74_000810"
     CDS             complement(22642..23817)
                     /gene="manA"
                     /locus_tag="N7C74_000810"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HGU2430742.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HKSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            24016..25662
                     /gene="fumB"
                     /locus_tag="N7C74_000811"
     CDS             24016..25662
                     /gene="fumB"
                     /locus_tag="N7C74_000811"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGU2430743.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSLSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQALGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            25805..27208
                     /gene="fumC"
                     /locus_tag="N7C74_000812"
     CDS             25805..27208
                     /gene="fumC"
                     /locus_tag="N7C74_000812"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HGU2430744.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(27205..28134)
                     /gene="tus"
                     /locus_tag="N7C74_000813"
     CDS             complement(27205..28134)
                     /gene="tus"
                     /locus_tag="N7C74_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HGU2430745.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSQALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(28210..29511)
                     /gene="rstB"
                     /locus_tag="N7C74_000814"
     CDS             complement(28210..29511)
                     /gene="rstB"
                     /locus_tag="N7C74_000814"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HGU2430746.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(29515..30234)
                     /gene="rstA"
                     /locus_tag="N7C74_000815"
     CDS             complement(29515..30234)
                     /gene="rstA"
                     /locus_tag="N7C74_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HGU2430747.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDIQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            30363..30698
                     /gene="ydgC"
                     /locus_tag="N7C74_000816"
     CDS             30363..30698
                     /gene="ydgC"
                     /locus_tag="N7C74_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HGU2430748.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(30695..31417)
                     /gene="folM"
                     /locus_tag="N7C74_000817"
     CDS             complement(30695..31417)
                     /gene="folM"
                     /locus_tag="N7C74_000817"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HGU2430749.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(31454..32836)
                     /gene="ydgI"
                     /locus_tag="N7C74_000818"
     CDS             complement(31454..32836)
                     /gene="ydgI"
                     /locus_tag="N7C74_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU2430750.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDIFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(33022..33966)
                     /gene="ydgH"
                     /locus_tag="N7C74_000819"
     CDS             complement(33022..33966)
                     /gene="ydgH"
                     /locus_tag="N7C74_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HGU2430751.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            34490..36022
                     /gene="pntA"
                     /locus_tag="N7C74_000820"
     CDS             34490..36022
                     /gene="pntA"
                     /locus_tag="N7C74_000820"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HGU2430752.1"
                     /translation="MRIGIPRERLTNEPRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGCFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKNGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQVAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            36033..37421
                     /gene="pntB"
                     /locus_tag="N7C74_000821"
     CDS             36033..37421
                     /gene="pntB"
                     /locus_tag="N7C74_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HGU2430753.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(37446..38480)
                     /gene="tqsA"
                     /locus_tag="N7C74_000822"
     CDS             complement(37446..38480)
                     /gene="tqsA"
                     /locus_tag="N7C74_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HGU2430754.1"
                     /translation="MAKPLITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSTLNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFKQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
BASE COUNT         9778 a         9592 c        10054 g         9458 t
ORIGIN      
        1 tgagatagtc cagtttctta aaaatagcca gtgtaatgtt ttgtaggtca atatttgttg
       61 cgtgtgtaaa tttaaatgat tttaacctga ttcttgcaat gtttttacat tgccacagca
      121 tcgtgatctt gatcactaat gaatagaaaa tttcgctatt aaaaaaacaa ttaacctgca
      181 tttttggtta tccgcaaacg gattacctga tgataacgct aagcaatggc atcattgacg
      241 ttaaaaagat gttttaaaag aggataaaga aaggttatca atggggtaat cggtgttacc
      301 ccttttcttc agatgtcaac tttctctttg tattcacaaa gatcttcaat aatacaagag
      361 ccacagcggg gcttgcgggc aatgcaggta taacgcccgt gcaggatcaa ccaatggtgg
      421 cagtcgactt taaactctgc cggaaccact ttcagtagct tttcttctac ctgttcgacg
      481 tttttccccg gcgcaaattg agtacgatta caaacgcgga aaatgtgcgt gtcgacagca
      541 atagtcggcc agccgaatgc agtgtttaat acgacgttgg ctgttttacg acctacgccg
      601 ggcagggctt caagcgcagc acgatcttcc ggaacctcgc tattatgctg ctccagtaag
      661 atacggcagg ttttgatgat attttctgct ttgctgttat aaagcccaat cgttttgata
      721 taggttttca ccccttcaac gcccagttca agcatcgctg caggcgtatt cgccaccggg
      781 tagagtttcg ccgtcgcctt attaacactg acatcggttg cctgagcgga aagcagtacg
      841 gcaatcagca attcaaaagg cgaactgaaa ttaagctcag tggtgggatg aggattgttc
      901 tcacgcaggc gagtgaggat ctccaggcgt tttgctttgt tcatcagaca ttccctgttt
      961 caccgtttgg caatgcacgt tcggcagctg cttcagtacg gcgctttttc attctttcat
     1021 caatcaggta ttttcctgcc agcatcagtc ccaggccaat aaatgcacct ggtggcagca
     1081 tcgccagcag gaaaggggag tcggtgtgga aaatctccac gcgtaatact tttgcccagc
     1141 tacctaacag cgcatctgca ccgtcaaaca atgtgccatt gccgataatt tcgcgtagtg
     1201 aacccagcac gaacatggcg caggttgcgc ccataccaat tgaaaagccg tccagtgccg
     1261 aaagcgccgg accttttttg gcagcgaagg cttcagcgcg gcccacaacg atacagttag
     1321 tgacaatcag cggaataaaa atccctaatg attgatacag gccaaaggcg taggcgttga
     1381 tcagcatctg tacagcgctg accaccgagg cgatgatcat cacgtaaatg ggaatgcgga
     1441 tctcggctgg cgtccagtga cgcagcgtcg aaatggtcag gttggtcagc gtcagtacca
     1501 gcgtagtcgc aagtcctaaa cccagagcgt tagtggcagt agacgtgaca gccaacagag
     1561 gacaaaggcc gagcaactgg accagcgcag agttgttttt ccacaacccc tgaacaataa
     1621 cgtctttaat ttcgctcacg ctttattctc cacaggcagg aagttgagaa agttgttccg
     1681 gtaacgtctg agcgtacaat ccggcgcgtt ttaccgcatt aaccaccgcg cggggagtaa
     1741 tcgtcgcgcc ggtgaactgg tcgaaattac caccatcttt cttcaccgcc cagtgcgcat
     1801 catccgcacc actgattttt ttacccgcaa aatgggtgat ccagtcagaa aggcgcagtt
     1861 cgattttatc gccaagccct ggcgtttcgt ggtgctctgt cacgcgcgtg ccaagtaccg
     1921 tgccgttaaa atcggctccc accagcagct gaatcgcacc ggaatagcca tctggcgcgg
     1981 ttgcttccag aacggcggct accggtttgt catcctgttt ggcgatgtaa acccgatgct
     2041 cacctttacc taactctggc gcagttacca gatagcaact ctgtgccagc gcattgttat
     2101 agcgttcggc tggcagcacc tgatcaaata aagccttttg ttgcagactg gcctgttcag
     2161 caatcgtcgt tttggtcatc tggttgatgg ccgcagttaa ccctgttgaa cccgctgcaa
     2221 atagcgccaa cgtaatgccg tgttttcgga tagttttcag catggtttac cctttgcgat
     2281 ggccgtagac gcgcggacgc gtgtagtaat cgatcagagg aaccgtgatg ttcgccagca
     2341 ggacggcaaa agccacgccg tcaggatagc cgccgaaact gcggatcaac cagactaata
     2401 agcccgcaag cgcgccgaaa ataagacgac cacgattggt cgtagaagcg gtaaccgggt
     2461 cagtcaaaat aaagaatgcg ccgagcatgg tcgctccaga cagcagatga atttgcggtg
     2521 ctgccagtgt ttctggtgag aacaaccagc ccaacgttgc gcataacgcc agcgttacta
     2581 agaagctgag gggaatatgc cagcgaatcg ctttctgcca tagcaaccat acgccgccag
     2641 ccagccaggc gagatttacc cattgccaac cagcgcccgc cagaataccg ctgtagatcg
     2701 gatattgcat aatctgttca accgaatgac cggcacggac agaggtttta aatgtatcca
     2761 gcggtgtcgc ctgactaatg ccatcaatac ctaagcgtag tgtgttcata tcaccaccac
     2821 tggcggtatg cccgctgaaa ataacctgta tggcgtcgat aaaaccaggg atgttgaccg
     2881 caatttcatg tggcggtaac cagctggtca tctgcaccgg gaaggagatc agtaagacca
     2941 cataaccaat cattgccgga ttaaacgggt tttgccccag accgccatac aactgtttag
     3001 cgatgataac cgcaaacacc gtacccagca cgaccatcca ccatggcgcg agggggggaa
     3061 tacttaccgc cagcaataag cctgtcagca atgctgagtt atctttcaac gtggcggcta
     3121 ccgactgctt gcgtagtttg agtacgagag cttcggctaa cagagcacta accgatgcca
     3181 acaggatctg aacgagagta ccccaaccaa aaaaccacag ttgcgctgcg attcctggca
     3241 cggctgcgag caacaccaac agcataatgc gcgatgtctg gcgctggtta tgggtataag
     3301 gggagctagc tattctgaat accatttagt cctcgtttac aaccttttgc tggacggctt
     3361 ttttggcctg aacgcgggca atagccgcgg caactgccgc tttgcgcgga tcaacctgtt
     3421 cttctggttc tgcattagcc tgttgctgtt ccagcttgcg tgctttggcg cgtgcaatag
     3481 ccgctgcgac ggcagctttg cgcggatcaa cctgttcttc tggctccgca ttagcctgtt
     3541 gctgttccag tttgcgcgct ttggcacggg taatagcggc ttcgacggcg gctttgcgcg
     3601 gatcgaccgg ttcttctggt tccgcattag cctgttgctg ttccagcttg cgtgctttgg
     3661 cacgggcaat agcggcttcg acggcggctt tgcgcggatc gaccggttct tctggttccg
     3721 cattagcctg ttgctgttcc agcttgcgcg ctttggcgcg ggcgatagcg gcttcgacgg
     3781 cggctttgcg cggatcgacc tgttgttctg gttccgcatt agcctgttgc tgttccagct
     3841 tgcgtgcttt ggcacgggca atagcggctt cgacggcagc tttgcgcgga tcgacctgtt
     3901 gttctggttc cgcatttgcc tgttgctgtt ccagcttgcg cgctttggcg cgggcgatag
     3961 ctgcttcaac ggcagtttta cgtggatcag taacggttgc tgcgtcgtta gtttgctgca
     4021 gttctgcctg tttcgctctg gcttgcgctt tacgggcttc ccgtgctgca ataattgcac
     4081 tgttatccgg gcgttcgccc gctttaatca caataggctg tgtagcctgg gcctgtttct
     4141 ctttcacccg cgccagagcg gcagcaatcg catctttatc tttggctgca ggttgaacgg
     4201 ctgcgctctt atgtcgttca aggcgagccg ctttttcgcg ctccagacga gcctggcgcg
     4261 cttcgaaacg cgctttggct tctgcggcgc gcttttcttc ctgacgaata gccgcaattt
     4321 cagctttttc ctgacggaaa tattgcacca ggggaatatt gctcgggcaa acccacgcgc
     4381 aagccccaca ttcaatgcag tcggcaatgt tatgcgtggt agctttatcg tgttgctgac
     4441 ctttgctgaa ccagtacaac tgttgcggca aaagatccgc agggcaggcg tcagcacagg
     4501 cgctacaccg gatgcagctt tgttcttcct gtggttcgcc aagctcattg gcagagggag
     4561 ccaacagaca gttggtaatc tttacgaccg ggacatccag ccatggcaag gtaaagccca
     4621 ttagcgggcc gcccataatc accatttgat cggcagaggg gcagaatccg gcgtcattca
     4681 ataaatgacg cactggcgtc cccagccgtg cccagacgtt gcccgggcga gcgattgctt
     4741 cgccagtcag ggttacgaca cgctcggtaa tcggctcgcc atcaataacg gcacgtttca
     4801 ctgcataagc agtgccgacg ttttgcatta atacgccgat atcggatgaa cgcccgccat
     4861 gtggaacctg cttcccggtc agaatgtagg ttaattgttt agcaccgcca gaaggatatt
     4921 tggttggaat cacccgcata gaaatatcgt gagagtccgc cagcaccgcg cgcagcatgg
     4981 aaatcgcctg cggtttgtta tcttcaatgc cgataagaat ttcgcgtggc tgcagaatat
     5041 gcgcaagaat gcgaataccc tctacgacct gagccgcgca atcctgcatc aaacggtcat
     5101 cggcggtaat gtatggctcg cactcagcag cgttgataat caacgtctca atcttatctc
     5161 cgccgccctg caatttaacg cctgtcggga atcctgcacc acccagcccg gcaacaccaa
     5221 actgatgaat gcgctcgatt aactcttcgc gacggcgaga gcgataatcg gcccagccgt
     5281 ctcgtgggat ccagcagtct tcaccatcgg catcaataat cacgcttaat tcagctaaag
     5341 ctgaaggatg agccgtagag tggggcgcaa tagccgtaac ggtacccgag gtgggcgcgt
     5401 gaacaggcag cattttgccg cgaccacggg taagcggctg gccgcgcaat actttatcgc
     5461 cgacgctaac gcacaactca ccttcagcgc caatatgctg tttcagtgga atgacaaaac
     5521 gctgcgccag tggtacctgg cgcaggggtg taccgttgga ctgggttttc atctccggtg
     5581 gatggatgcc gccgttgaaa tcccagattt tattttttct gaatgcagag aataacttaa
     5641 gcatggtgtt ccacgggaat gatacgcacg ggaatggtgt tcagatccca tttccaggag
     5701 tcaggtgttt ctgcgaccgg ttgcaacgag atgcagtgcg tcgggcacgg atcaacacat
     5761 aaattgcagc ccgtacagag atcactcatt accgtatgca tggcacgggt agcgccaacg
     5821 atggcgtcta ccggacacgc ctgaatacat ttagtgcagc caatacagtt attttcatca
     5881 ataaccgcca ccatccgcgc aggcgttagc tcttgcgctt cgccatccag cggctgcggc
     5941 tcgacattaa gcaactcggc aatttttagc atcacagctt cgccaccagg ggcgcaacgg
     6001 ttgatttttt caccgttaca gctgatggct tccgcgtagg ggcgacagcc gggataaccg
     6061 cactgaccac actggctctg cggtaagatt tcgtcaattt tctcaacgac cggatcgtct
     6121 tccaccgcaa aacggcggga ggcataaccc agaatggcgc caaacgccag gcccagcagg
     6181 ctcacggcgg caacggcaat ccagatagca ttcattacaa cttcaccaaa ccactaaagc
     6241 ccataaaggc cagagacata agacctgcgg taattaacgc aatggcatta ccgcgaaaag
     6301 gtgccgggac atcagccaca gcaaggcgtt cgcggatggc tgcgaagaga accatcacca
     6361 gcgaaaaacc gacagcggcg gaaaaaccgt acagcgccga ctgcaagaaa ttgtgcccga
     6421 gattgatatt cagcaacgcc acgccgagca cggcacagtt ggtggtgata agcggcaaaa
     6481 aaatccccag caaacggtaa agcaccgggc tggttttgcg caccaccatc tcggtgaact
     6541 gcacgaccac agcaatcacc agaataaatg ccagggtgcg caggtaaatc agattaagtg
     6601 ggatcaaaat ccacgtatcg ataagccagg cgcaaataga cgccagcgtc atcacaaacg
     6661 ttgttgccag ccccatgccc atcgcggttt ccagcttttt ggaaaccccc ataaacggac
     6721 agagaccgag aaacttgacc agtacaaagt tattgaccag tacagttccg acaaacagta
     6781 acaggtagtc agtcattttt cggcctgaaa taaaaaagcc gcctattatc cgttaatcca
     6841 gagcaggcga caacaggtta actgtaaggt tattacggat tcacaaacgt cgctttcacc
     6901 cgcgacgagc gtttgaaata ggggacgatc aatgcggttg ccaacaaagt aaacagcaac
     6961 tggcgaacgg ctatgccatc ttccacgggt gaaaaagcga acgcttttac cgccagcaat
     7021 acggaaataa gcagccagat aatatagtgt ttaggaacac aacgccgacg tttaaagaaa
     7081 gcgatggtca accacagtgt gtaataccac atggcgatag cggtaataaa agaaacgccc
     7141 cataagattt gtgtcgtcag tgcttgtgcg ccgagcgttt gaaatgtttg cggagaagat
     7201 aacgcagcgg tatacagcaa caacgccagc gtcgtactta acagagcgac taaaagccag
     7261 gccaacgggc caagcaacca gcctccaata cgttgtggcg ttgtggtggt cataggttct
     7321 ccagaatagc gacgcaaaca ggcaaatcta attttagggg cgttattata aggcttttat
     7381 aaccgatcgc tactttcttt attgaacata gtgccagacg gacttcggta cctggccgag
     7441 gtcaaacaaa cgcccgccag aaaccagctc tgcgcgacgg tgatcggcag cacgatacat
     7501 attgatcagt tcctgatcgt ccgtcagggt gtaattaaga tggtcgtaga gtttttccag
     7561 actttcgagt gaactgattt tgcgaaattt taataagtag tcctgaacag tcataaaagg
     7621 tccataaaat aattaatacc aaaggggtag gtattattag tgatgataaa atttcaccaa
     7681 aagtgaacgc ttgtgttatt acaataataa gaatgcgctg atggcaaata ttcagggatg
     7741 aaaaactgct atgaaaagag taacgcgctt tactatccct gacaatcacc aacaacaccg
     7801 aacaagataa taaattcctg gtttaatatc cgacaagtga aaacatgcac cctgacgggc
     7861 agcatgtcgc tctacaagcg cagggcttac ttgtgttgta ccgtagtact ctgttcaggt
     7921 ggctgatagt tgtcaatgtg actcgccacg ccaagaagaa tgactgaaac gacaagaacg
     7981 atccaacctg ttaattcaat aagacgattc attacaactc acatctctgt tgattgatcc
     8041 attaacttca gggggtaaat gttacttagc aataatagct ccagcaagat ttttactgag
     8101 gtttttgcga tatttagctt ttgtcgttgg aaaaatcgct atttttttga cttaagttaa
     8161 acaacatccc ttattgctgg caggttatta aactgttgag cgagggtaaa ggatagtgtc
     8221 aaatagccat catacttcaa tgagaggcaa tgacatgagc gacaacatcc gtgttgggtt
     8281 gattgggtat ggttatgcga gcaaaacctt ccatgcgccc ctgattgcgg gcacacccgg
     8341 gctggaactg gcggtaatct ccagcagtga tgaaacaaaa gtaaaagccg actggccaac
     8401 ggttacggtt gtctctgagc cgaagcatct gtttaacgat cccaacatag acctgattgt
     8461 cattcctaca cccaacgata cccatttccc gttagccaaa gcagcgcttg aggcgggtaa
     8521 acatgtggtc gttgataaac cctttaccgt gacactgtca caagcgcgag agctggatgc
     8581 gctggcaaaa agcctggggc gtgttctgtc tgtattccat aaccgtcgtt gggatagcga
     8641 tttcttgacg ctaaaaggtt tgctcgcgga aggcgtgctg ggtgaagttg cttactttga
     8701 gtctcatttt gaccgcttcc gtccgcaggt gcgcgatcgt tggcgtgaac agggcggccc
     8761 aggcagcggt atctggtacg atttagcacc acatcttctt gatcaggcca ttatgctatt
     8821 tggtttaccg gtcagcatga cggtagattt ggcacagtta cggcccggag cgcagtcgac
     8881 cgattatttc cacgccatct tgtcctatcc acagcggcga gtcattttac acggtaccat
     8941 gctggcagcc gctgagtcag cacggtatat cgtgcatgga tcccgaggca gttatgtgaa
     9001 atatggcctc gatccacagg aagaacgtct gaaaaatggc gagcgtctgc cgcaggaaga
     9061 ctggggctac gatatgcgtg atggcgtact tacccgcgtg gaaggtgagg aacgtgtcga
     9121 agaaacgctg ttgacggtgc ctgggaatta tccggcttac tatgcggcga ttcgtgatgc
     9181 gttaaatggc gatggtgaaa atccggttcc ggcaagccag gcaatccagg taatggagtt
     9241 gattgagctg ggcatcgaat ccgccaaaca tcgcgcgact ttgtgccttg catgaaaaat
     9301 ggatggtgca atttgcacca tccatttttg ttacttcgct gcgacttttt ctcgcagtgc
     9361 gcgtttttcc tctgcgttga ggaaagccat ttccagacca ttaatctgtg cctggcggat
     9421 ttgctcgcgg gataacccag cagctggcgc ggcaacggta tattcgtgaa tgatatccac
     9481 tccctgtacg ccgggatcgt cagtgttaat gctggcacga atgccatgct caaggaacgt
     9541 tttcagcgga tgtgcagcca gctctgccac ggtgctggtc tgaatattgg aggtcagaca
     9601 ggattcaata ccaatttgtt gctcggcgag aaaatccatc agcgcccgat cttcaatggc
     9661 ttttacgcca tgtccaatgc gctctgcccc cagttcacga atcgcctgcc agatgctttc
     9721 cggcccggca gcttcgcctg catggacggt aatatgccag cccgcatcac gcgcgcggtt
     9781 gaagtgagaa agaaacagac ttcccgggaa accaagttca tcaccggcta aatcaagtgc
     9841 ggtaatctgg tcacggtggg ctaaaaaggc ctccagctct tgctgacagg cggcttcgcc
     9901 gaaggttcgg ctcataatgc cgataagctt cgcctgcaca ccaaaggtgc ggcaaccttc
     9961 acgtacgcca tcgatcaccg cttcaacaac acctgcaaca ggcagctgat gtgccattgc
    10021 catgtagcct ggtgaaaaac gcagctcgac atagtgcagg ccgttacggg ctgcatcttc
    10081 aatgttttca aatgccacgc ggcgacaagc atcaagagag gcgagaactt taacgcccca
    10141 gtcaagtttg gtcagaaagc tcaccagatc gggttcgttg gcaatgacct gaacgtgggg
    10201 aatcagtgtt tccagggatt gtgcaggaag cgagatatta tactggcggc caagttcaag
    10261 aatggtctgg ggacgaatgt tgccatcaag gtggcgatgg atatcagtta atggcagggt
    10321 ggtatcaatc atggtcgcac tctttttctg gatataaaag tgcgcaccat tataaaaaca
    10381 aagcagggta aaaatccacg attgcgcaac gattttttac gttgcgcaat tggggttagc
    10441 gaacagcgcg gatggcgtta attaatccag ccacaccttt ttccagtttc gaacgtgggc
    10501 agccggcatt gagtcggaca aaaccacgac cttcttcacc gtaggtatac cccggcatga
    10561 tcgcgacttt ttcttgttcg ataagtgctt tttgcaacgc gttgtcgtca atattcaacg
    10621 gacgtaaatc aagccaggcc agataagtgg attgcgggat ctgccagttg agttcaggaa
    10681 acgcggcgtt cattttatct gcgatatacg tcaggttatc tttcagatag acgcgtaagg
    10741 catccagcca cggtgcacct tgctgatagg cggcgatatg ggcagttaac gccagtaccg
    10801 aaggggaaga aagcccatca cgtcctttca gtgccgataa ataggcatca cggctgctgc
    10861 tattttctat aatcccgtaa gcaccggtca gcgcgggaat attgaaactt ttcgatcccg
    10921 acgtcagcaa cgcccagtct ccccgcgcca cattactcca gggaatatgc ggctgctcgc
    10981 cccaaaccat atccatatgg atttcatcgg aaataacccg cacaccatga cgctcgcaca
    11041 ggtcagccat gatctccagc tcatcgcacg tccacacttt tccggtagga ttctgtgggc
    11101 tacacaggag cataatttta cattctggtt tcgccaacac ggcttccagc ttgcccatat
    11161 cgcaaaacca gccgtcagcc tgcttctcta aagcaacggg cattactgtg cgctgattac
    11221 cttcaatggc cttgtaaaat gcgtcatagg cgggtgtgtg gatcaccacg ccttcacctg
    11281 tttcagacca ctgacgaatc agttctgaga ccatatagat aacagaaggg ccatacacca
    11341 ccgactgagg atcgatggcg gtgtaatgct gggtggaaaa ccagtgggca atagccgcga
    11401 gaaactcatc gttcttccag cggctgtagc caaacacgcc gtgcatcagg cgctgattca
    11461 gcgcctcgat aatgcagggg gcagtggcaa aatccatgtc tgaaatcgtg aaaggtaaca
    11521 ggtcagcagt gccgaaacgg tcagcgacat aatcccactg cgtacaccat gtgccatgac
    11581 gatccacgac ctttgaaaaa tcgaacatat cttatcctta tgcctggaca gtatgcatca
    11641 gaccggccat ttcatctttt actgactgaa cttgtggccc gataacaacc tgcaggttat
    11701 gttgattaag ttgtactacg ccaattgccc gattgtcctt cagtgcctgc acattaacaa
    11761 gcgacatatc tttcacagac aaacgcagac gggtaatgca gttatcgagg ctaacaatat
    11821 tgtcggcacc gcctaaggct tcgaggattg ccggaacgtt gtaacctgat ttacccggcg
    11881 caccggcaac ggctttttcg attgaactgg ccacttcgct atcgcgcccc ggggttttca
    11941 gattgaagcg ggtgatagcg aaacggaaga tgacgtagta aacgacaaac cagattgcca
    12001 ccaccactgg caccatgtac cacttggttg acagaccatg caaaataccg aacaccacga
    12061 agtcgatgat attgccgtcg gtattaccga tggtgacgcc gagcacagac atgacggtga
    12121 agccgaggcc ggttaacagc gcgtggatga catacagaac tggcgctacg aacaggaaca
    12181 ggaattccag cggttcggtc gtgccgccaa cgacacaggc gatcaggcca gaaatcagca
    12241 gccctttaat tttatggcga ttttccgggc gcgcgcagtg atacatcgct aacgctgcac
    12301 ctggcagacc gccgagaaac gcaggcattt tgccttgcga aaggaaacgc gtggcgcttt
    12361 cagaaaaacc gtgagtggtc gggcaactca attgcgcctg gaagatggtc aatgcgccgc
    12421 tgacggtttg accgcagact tcctgcgtgc cacctgcgtc ggtaaagcga attaatgcca
    12481 ccagaatgtg atgcagacca aacggcaaca gcagacgttc accggtacca aacagcatcg
    12541 gtccgaaatc acccgcgcta ttgatcatat ggcctaagcc gctaataccc atggcgaaaa
    12601 tcggccagac taatggaatc accaggccga caaggcccat caccagcgag gagatgattg
    12661 gtacgaagcg cgtaccgccg aagaatgcca gcgcatccgg caggcggata ttatggaatc
    12721 gctcatgcag catccagacg ataataccgg cgatcaccgc accgaggatc ccggtgtcga
    12781 tcgactggat cccaagaatg ctctggatgt tattggcttt cagaacggcg gcatccgtgg
    12841 ttggcagaat gcctttattg gtcaaccaga agtttaccgc gaggttcatt accgcataac
    12901 cgacgaagcc agcgaatgcc gctacgcctt tgttttctcg tgccagaccc agcgggatgg
    12961 cgatacagaa catgactggc aggaaactaa aagcaaacga gccaatctta ctcatccagg
    13021 taaagatagc ctgcaacacg gggttgccca ggaccgggag cagggtgatg acatcgtggc
    13081 tactaaggga actaccaatg ccgagcataa tgccgcagaa cgacaataat gccacgggta
    13141 acatgaaggt tttgcctaac tgctggaaga actcccacag cgtgactttc ggtgctgttt
    13201 tcgccgtcat aaaacgactc ctcgtagaga acaatctgga ttcagtaaat tgcgcgagaa
    13261 gataaaacgt tttatcaaat tttagtgagg cataaatcac attacgcaac gataatagcg
    13321 ggtataagat aaataaaagg taaaacgttt tatctgtcac aaaatcaggg agtaggtcat
    13381 ctgcatggct accgccaaaa aaataaccat tcatgatgtt gcgctggctg cgggcgtgtc
    13441 ggtaagtacc gtttcgctgg tgcttagtgg caaagggcga atctctaccg ccacaggaga
    13501 acgcgttaac gccgccattg aagagctggg atttgtgcgc aatcgccagg cgtcggcgct
    13561 gcgcggtggg caaagcggcg tcattggttt gatcgtccgt gatttatctg cgccgtttta
    13621 cgccgaattg acggccggat tgacggaagc tctggaagcg cagggacgga tggttttttt
    13681 ggttcacggc ggtaaagacg gcgagcagct ggcacagcgg ttttcactgt tactgaatca
    13741 gggtgtcgat ggtgtggtaa ttgccggggc tgcaggaagc agcgatgacc tgcgacggat
    13801 ggcagaagaa aaagctatcc cggtgatttt cgcttcccgt gccagttatc ttgatgatgt
    13861 tgatacggtt cgcccggaca acatgcaggc tgcacagttg ttgacggagc atctcattcg
    13921 caatgggcat cagcggatcg cctggctggg agggcaaagt tcctcattaa cccgtgcaga
    13981 acgggtgggg ggctattgtg caactctact aaaatttggc ctgccgtttc acagcgattg
    14041 ggtgttggag tgcacttcca gccagaagca agccgcggaa gctatcacgg cgcttttacg
    14101 tcataacccg accatcagtg ccgtggtttg ctataacgaa actattgcga tgggggcatg
    14161 gtttggtttg ctgaaagcag ggcggcaaag cggggaaagc ggagtcgatc gttactttga
    14221 gcaacaggtt tcgctggcgg catttaccga tgcgacacca accacacttg atgatatacc
    14281 cgttacctgg gccagcacgc ctgcgcggga acttggtacc acacttgcgg atcgcatgat
    14341 gcaaaaaatc acccatgaag agacgcattc acgcaatctt attattcccg cccggctcat
    14401 tgcggcgaaa taatcctctc tttatctgct atacctggta gtgtcctttc ctcaagttta
    14461 atgataaaaa atgtggcaca caggcggtgc cttttatttt tactgaagtt cacaggaggt
    14521 ttatgtgttt aattctgaca acctgagact cgacggaaaa tgcgccatca tcacaggtgc
    14581 gggtgcaggt attggtaaag aaatcgccat tacattcgcg acagctggcg catctgtggt
    14641 ggtcagtgat attaacgccg acgcagctaa ccatgttgta gacgaaattc aacaactggg
    14701 tggtcaggca tttgcctgcc gttgtgatat tacttccgaa caggaactct ctgcactggc
    14761 agactttgct atcagtaagc tgggtaaagt tgatattctg gttaacaacg ccggtggcgg
    14821 tggacctaaa ccgtttgata tgccaatggc ggattttcgc cgtgcttatg aactgaatgt
    14881 gttttctttt ttccatctgt cacaacttgt tgcgccagaa atggaaaaaa atggcggtgg
    14941 cgttattctg accatcactt ctatggcggc agaaaataaa aatataaaca tgacttccta
    15001 tgcatcatct aaagctgcgg ccagtcatct ggtcagaaat atggcatttg acctgggtga
    15061 aaaaaatatt cgggtgaatg gcattgcgcc gggggcaata ttaaccgatg ccctgaaatc
    15121 cgttattaca ccagaaattg aacaaaaaat gttacagcac acgccgatca gacgtctggg
    15181 ccaaccgcaa gatattgcta acgcagcgct gttcctttgc tcgcctgcag ccagctgggt
    15241 aagcggacaa attctcaccg tctccggtgg tggggtacag gagctcaatt aatacactaa
    15301 cgaaccggta aacagccgta tgtgttgttt accgggatag cgtgtgcaat tcatcaacgt
    15361 ctctgctaaa caactggcag ccaaatcacg gccattggtt aaccaatagc tggatgaaaa
    15421 gcatacgaat agagtgtgcc ttcgcactat tcaacagcaa tgataggcgc tcacctgaca
    15481 acgcggtaaa ctagttattc acgctaacta taatggttta atgatggata acatgcagac
    15541 tgaagcacaa ccgacacgga cccggatcct caatgctgcc agagagattt tttcagaaaa
    15601 tggatttcac agtgcctcga tgaaagccat ctgtaaatcc tgcgccatta gtcccgggac
    15661 gctctatcac catttcatct ccaaagaagc cttgattcag gccattatct tacaggacca
    15721 ggagagggcg ctggcccgtt tccgggaacc gattgaaggg attcattttg ttgactatat
    15781 ggtcgagtct attgtctctc tcacccatga agcctttggg caacgggcgc tggtggtcga
    15841 aattatggcg gaagggatgc gtaacccaca ggtcgccgcc atgcttaaaa ataagcatat
    15901 gacgatcact gaatttgttg ctcagcggat gcgtgatgcc cagcaaaaag gcgagataag
    15961 cccagacatc aacacggcaa tgacttcacg tttactgctg gatctgacct acggtgtact
    16021 ggccgatatc gaagcggaag acctggcgcg tgaagcgtcg tttgctcagg gattacgcgc
    16081 gatgattggc ggtatcttaa ccgcatcctg attctctctc tctttccgcg ggctggtgac
    16141 aactgagccc gcgtttcata ctttaatttc cctgtacaaa atacaatctc gccggagcgg
    16201 ggaattcctc cctaaaatat tcaccgtagc catatgtcat gagagtttat cgttcccaac
    16261 atgctcgaac gaacgttcgg ttgcttattt tatggcttct gtcaacgccg ttttaaagat
    16321 taatgcgatc tatatcacgc tgtgggtatt gcagtttttg gttttttgat cgcggtgtca
    16381 gttcttttta tttccatttc tcttccatgg gtttctcaca gataactgtg tgcaacacag
    16441 aattggttaa ctaatcagat taaaggttga ccagtatttt tatcttaatg aggagtccct
    16501 tatgttacgt cctgtagaaa ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc
    16561 attcagtctg gatcgcgaaa actgtggaat tgagcagcgt tggtgggaaa gcgcgttaca
    16621 agaaagccgg gcaattgctg tgccaggcag ttttaacgat cagttcgccg atgcagatat
    16681 tcgtaattat gtgggcaacg tctggtatca gcgcgaagtc tttataccga aaggttgggc
    16741 aggccagcgt atcgtgctgc gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa
    16801 taatcaggaa gtgatggagc atcagggcgg ctatacgcca tttgaagccg atgtcacgcc
    16861 gtatgttatt gccgggaaaa gtgtacgtat cacagtttgt gtgaacaacg aactgaactg
    16921 gcagactatc ccgccgggaa tggtgattac cgacgaaaac ggcaagaaaa agcagtctta
    16981 cttccatgat ttctttaact acgccgggat ccatcgcagc gtaatgctct acaccacgcc
    17041 gaacacctgg gtggacgata tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc
    17101 gtctgttgac tggcaggtgg tggccaatgg tgatgtcagc gttgaactgc gtgatgcgga
    17161 tcaacaggtg gttgcaactg gacaaggggc accagcggga ctttgcaagt ggtgaatccg
    17221 cacctctggc aaccgggtga aggttatctc tatgaactgt acgtcacagc caaaagccgg
    17281 acagagtgtg atatctaccc gctgcgcgtc ggcatccggt cagtggcagt gaagggcgaa
    17341 cagttcctga tcaaccacaa accgttctac tttactggct ttggccgtca tgaagatgcg
    17401 gatttgcgcg gcaaaggatt cgataacgtg ctgatggtgc acgatcacgc attaatggac
    17461 tggattgggg ccaactccta ccgtacctcg cattaccctt acgctgaaga gatgctcgac
    17521 tgggcagatg aacatggcat cgtggtgatt gatgaaactg cagctgtcgg ctttaacctc
    17581 tctttaggca ttggtttcga agcgggcaac aagccgaaag aactgtacag cgaagaggca
    17641 gtcaacgggg aaactcagca ggcgcactta caggcgatta aagagctgat agcgcgtgac
    17701 aaaaaccacc caagcgtggt gatgtggagt attgccaacg aaccggatac ccgtccgcaa
    17761 ggtgcacggg aatatttcgc gccactggcg gaagcaacgc gtaaactcga tccgacgcgt
    17821 ccgatcacct gcgtcaatgt aatgttctgc gacgctcaca ccgataccat cagcgatctc
    17881 tttgatgtgc tgtgcctgaa ccgttattac ggttggtatg tccaaagcgg cgatttggaa
    17941 acggcagaga aggtactgga aaaagaactt ctggcctggc aggagaaact gcatcagccg
    18001 attatcatca ccgaatacgg cgtggatacg ttagccgggc tgcactcaat gtacaccgac
    18061 atgtggagtg aagagtatca gtgtgcatgg ctggatatgt atcaccgcgt ctttgatcgc
    18121 gtcagcgccg tcgtcggtga acaggtatgg aatttcgccg attttgcgac ctcgcaaggc
    18181 atattgcgcg ttggcggtaa caagaagggg atcttcaccc gcgaccgcaa accgaagtcg
    18241 gcggcttttc tgctgcaaaa acgctggact ggcatgaact tcggtgaaaa accgcagcag
    18301 ggaggcaaac aatgaatcaa caactctcct ggcgcaccat cgtcggctac agccttggtg
    18361 acatcgccaa taacttcgcc ttcgcaatgg gggcgctctt cctgttgagt tactacaccg
    18421 acgtcgctgg cgtcggtgcc gctgcggcgg gcaccatgct gttactggtg cgggtattcg
    18481 atgccttcgc cgacgtcttt gccggacgag tggtggacag tgtgaatacc cgctggggaa
    18541 aattccgccc gtttttactc ttcggtactg cgccgttaat gatcttcagc gtgctggtat
    18601 tctgggtgcc gaccgactgg agccatggta gcaaagtggt gtatgcatat ttgacctaca
    18661 tgggcctcgg gctttgctac agcctggtga atattcctta tggttcactt gctaccgcga
    18721 tgacccaaca accacaatcc cgcgcccgtc tgggcgcggc tcgtgggatt gccgcttcat
    18781 tgacctttgt ctgcctggca tttctgatag ggccgagcat taagaactcc agcccggaag
    18841 agatggtgtc ggtataccat ttctggacga ttgtgctggc gattgccgga atggtgcttt
    18901 acttcatctg cttcaaatcg acgcgtgaga atgtggtacg tatcgtggcg cagccgtcat
    18961 tgaagatcag tctgcaaacc ctgaaacgga atcgcccgct gtttatgttg tgcatcggtg
    19021 cgctgtgtgt gctgatttcg accttcgcgg tcagcgcctc gtcgttgttc tacgtgcgct
    19081 atgtgttaaa tgataccggg ctgttcactg tgctggtact ggtgcaaaac ctggttggta
    19141 ctgtggcatc ggcaccgttg gtgccgggga tggtcgcgag gatcggtaaa aagaatacct
    19201 tcctgattgg cgctttgctg ggaacctgcg gttatctgct gttcttctgg gtttccgtct
    19261 ggtcgctgcc ggtggcgttg gttgcgttag ccattgcctc aattggtcag ggcgttacca
    19321 tgaccgtgat gtgggcgctg gaagctgata ccgtagaata cggtgaatac ctgaccggcg
    19381 tgcgaattga agggctcacc tattcactat tctcatttac ccgtaaatgc ggtcaggcaa
    19441 tcggtggttc aattcctgcc tttattttgg gattaagcgg atatatcgcc aatcaggtgc
    19501 aaacgccgga agtaattatg ggcatccgca catcaattgc cttagtacct tgcggattta
    19561 tgctactggc attcgttatt atctggtttt atccgctcac ggataaaaaa ttcaaagaaa
    19621 tcgtggttga aattgataat cgtaaaaaag tgcagcagca attaatcagc gatatcacta
    19681 attaatattc aataaaaata atcagaacat caaaggtgca actatgagaa aaatagtggc
    19741 catggccgtt atttgcctga cggctgcctc tggccttacc tctgcttacg cggcgcaact
    19801 ggctgacgat gaagcgggac tacgcatcag actgaaaaac gaattgcgca gggctgataa
    19861 gcccagtgct ggcgcgggaa gagatattta cgcatgggta cagggaggat tgctcgattt
    19921 caatagtggt tattattcca atattattgg cgttgaaggc ggagcgtatt atgtttataa
    19981 attaggtgct cgtgctgata tgagtacccg gtggtatctt gatggtgata aaagttttgg
    20041 ctttgccctc ggggcagtaa aaataaaacc cagtgaaaat agcctgctta aattaggtcg
    20101 cttcgggacg gattatagtt atggtagctt accttatcgt attccgttaa tggctggcag
    20161 ttcgcaacgt acattaccga cagtttctga aggagcatta ggttattggg ctttaacgcc
    20221 aaatattgat ctgtggggaa tgtggcgttc acgagtattt ttatggactg attcaacaac
    20281 cggtattcgt gatgaagggg tgtataacag ccagacggga aaatacgata aacatcgcgc
    20341 tcgttctttt ttagccgcca gttggcatga tgataccagt cgctattctc tgggggcatc
    20401 ggtacagaaa gatgtttcca atcagataca aagtattctc gagaaaagca taccgctcga
    20461 cccgaattat acgttgaaag gggagttgct cggcttttac gcgcaactcg aaggtttaag
    20521 tcgtaatacc agccagccca atgaaacggc gttggttagt ggacaattga cctggaatgc
    20581 gccgtgggga agcgtatttg gcagtggtgg ttatttgcgc catgcaatga atggtgccgt
    20641 ggtggatacc gacattggct atcccttttc attaagtctt gatcgtaacc gtgaaggaat
    20701 gcagtcctgg caattgggcg tcaactatcg tttaactccc aatttacact gacatttgca
    20761 ccgattgtga ctcgcggcta tgaatccagt aaacgagatg tgcggattga aggcgcgggt
    20821 atcttaggtg gtatgaacta tcgggtcagc gaagggccgt tacaagggat gaatttcttt
    20881 cttgctgccg ataaagggcg ggaaaagcgc gatggcagta cgctgggcga tcgcctgaat
    20941 tactgggatg tgaaaatgag tattcagtat gactttatgc tgaagtaaaa aataacgccg
    21001 gagagaaaaa tctccggcgt tttggctgtg aattactgct gcggaatagc gggtacgacc
    21061 ggaacattta atgccggcat tgcaaacata ccaacgaaat cttccagtgg cattttctgc
    21121 ccgtttaacg ttatctgacc gttagtatat tgcaggctgg tggtgatggt attgtcctgc
    21181 aaggtggtca gacggaacat ctgccccatt gctgatgcac cttcaacttg ctgtttcgcc
    21241 agttttttcg cttgatcttc ctgataacct tccagcttcg ctacctgagt catcaactca
    21301 gttgccatat ccaccggaat ggtcagtttc gcatccagag atttaaccga acgatctact
    21361 tcctgcgcca gcgtttgcgg cgcttcttta gtcgttgccg gatctttcag gaacagcgac
    21421 agattcagcg cactttcacc ctgactgttt ttccagctta gcggcgcgat agtaatcacc
    21481 ggatcgcctt tcaacatcag cggcagggcg ctaaagaagg cttccgtcac tttctcctga
    21541 taaagttcgg gattgttggc aatttctggc tgtgccagca gcgcctgagt ttgcgcgtta
    21601 tattgctggc taaactgatg ccaggcttcg ccatcaattt ggccgacttt taaagtcagc
    21661 ttgccgctgc ccagatcctg attctgtacc ttcaggctgt ttagcgagta atccagttgg
    21721 ctattgatcg ttttaccgtc attgaccaga tccgatttac cgctgatctc catgccttcc
    21781 agcagtgcca gttctttgcc ttccactgaa atggtcattt ttaccagtga cagtttttgg
    21841 tttcctacgc gctcaccaaa acttgccagc gtgctggaac cgtcggtttt cagattatta
    21901 aaggtcaact gtactttctg gttgtattcg ttaaccgcgt ctatccgacc actttgcgcc
    21961 tccccggaaa gggagatagc tttgccgtct ctgtccgcat ttaactggaa ctcgccgccg
    22021 ctaaaggcga ctttttcatc cttttgctcg taatttagtg gcttgagcga aatatcggaa
    22081 ctggaatcac cgctgtaacc aatgcgcgag ttaatctcaa aaggcgtttc accttttgcc
    22141 atatcaaaca gtggtttgct tacttcgtta ttaaccagcg tggtttgaat tgatgccatc
    22201 gacgggatca ggttcagttt tttaagctgg gcaaggggga agggaccatg atcaaccgat
    22261 tcgttgaaga tgacgctctg accgctttta atccacggat tttcttttcc ggcaatgggt
    22321 ttcaccaaca gttgcagctg actgctgaat acgccgcgat gatagttttg ataactcact
    22381 tccaggttgg attcaggagc ggtcagtttg agttgcgcgt tcgcctgcgc gaccatgtct
    22441 tcgagatggg tttcaatctt cttgcctgta taccatgcgc cgcctgtcca gactacgcct
    22501 agcgcaacaa tgacgcctac cgctaccagc gatttattca taatgattat ccataaaatg
    22561 aaatcaggcg gactggccgc ctgaaggtgt tataagcctt taataagctt agcaagagat
    22621 gttaattttt tcagtaagct cttacagctt gttgtaaacc cgtgctaaac ggccgtggcc
    22681 tttgacagtc accggtgatt cgttggcggc aataaacgct gattcacccg gtttaagctg
    22741 taactgctga gaacctttcc acaacgttgc atcgccttcg acgcagaaca aaatggcggc
    22801 actctgctgg ctaatggtgg tttctttatc actaaggtca tgcagcgaga aggcaaaatc
    22861 atcaacagga atcgggaagt ccagttctgc accttgtttc accggctggg tcaacaactg
    22921 gttagccggt ttggcttcga acttcacatt ggcaaccagt tccggaatat caatgtattt
    22981 aggcgtcaga cccgcacgca gcacgttatc ggagtttgcc atcacttcca gcgccacgcc
    23041 ttgcaggtaa gcgtgcggtg tttcagcgaa caggaacatc gcttcgccag gatttaattt
    23101 caccacattc agcaacagcg gagagaacag gccgctgtca tccgggtaaa attcagaaat
    23161 taaacgaatc gtttgccacg gttcaccctg ctgactgtcg agcgccgatt ttaaaatcgc
    23221 cagcgcgcgg gatttttctt caccctgcat attcaacagg ctggcgaaca gttcgcttaa
    23281 acgttcggca tcaggctgtt gtaaaaagtg agcaatcgcc ggatgtgcac ctgcgaccgg
    23341 ctggagtagg gagacaatct cggaaaattc acgaaacgcg ttcatcgcaa ggaaaggcgt
    23401 cagcgcaaaa accagctccg gcttgtggtt aggatcttta tagttacgct cggcggcatc
    23461 catcgggata cctgcggcat tttctttggc aaaaccgatt tcagacttgt gtttgtttgg
    23521 atgaacctga atggagagtg gctgtgctgc gcataatact ttgaacagga aaggcagttc
    23581 gccaaagcgt ttggcaacgg cctctccgag cagagtcgat ttatcactct caatcacatc
    23641 acgcagtgaa acgatatctc cggcggcatt ctgcactcgt gaactgcttt tcggatgtgc
    23701 gcccatccac agctcggcca tcggctggct ggacggattt tccataccat aaagttcagt
    23761 caacgccgtt ttgctgcccc aggcatagtt ttgcactgag ttaatgagtt tttgcatgat
    23821 caatccctgt tttaatgtgg aaattaatcc cgctattaaa gcaagaatcc tacgggaagt
    23881 aacctggagc cgcaaaaagt cgtactagtc tcagtttttg ttaaaaaagt gcgtaggata
    23941 ttgttactcg cttttaacag ggcaacggaa cacccgccca gagcataacc aaaccaggca
    24001 gtaagtgaga aaacaatgtc aaacaaaccc tttcattatc aggctccttt tccactcaaa
    24061 aaagatgata ctgagtatta cctgctaacc agcgaacacg ttagcttatc tgaatttgaa
    24121 gggcaggaga ttttgaaagt cgcacccgaa gcgttaactc tgttggcgcg ccaggcgttt
    24181 catgatgcgt cgttcatgct gcgtccggcg caccaacaac aggtggccga cattctgcgt
    24241 gacccggagg ccagcgaaaa tgataaatat gtggcgctgc aattcctgcg taactccgac
    24301 atcgcggcga aaggcgttct gccaacctgt caggataccg gcaccgcgat tattgttggt
    24361 aaaaaagggc agcgtgtatg gaccggtggt ggtgatgaag cggcgctggc gcgcggtgtc
    24421 tataacactt atatcgaaga taatctgcgc tactcgcaaa acgcgccgct ggatatgtat
    24481 aaagaagtga ataccggcac caatctgcca gcgcagatcg atctttatgc cgttgatggc
    24541 gacgaataca aattcctctg tatcgccaaa ggtggtggtt cggcaaacaa gacgtatctc
    24601 tatcaggaaa ccaaagcgtt actgacgccg gggaaactga aaaattacct ggttgagaag
    24661 atgcgcacgc tgggtacggc ggcctgtcct ccgtatcata ttgcgttcgt tattggtgga
    24721 acttctgcag aaacgaacct taaaacggtg aaactggctt ccgcgaaata ctatgatgaa
    24781 ctgccaacgg aagggaatga gcacggtcag gcgttccgcg atgtggaatt ggaaaaagaa
    24841 ttgctgatcg aagcgcaaaa tcttggtctg ggtgcgcagt ttggcggtaa atacttcgct
    24901 cacgacatcc gcgtgattcg cctgccacgt cacggcgcat cctgcccggt cggtatgggc
    24961 gtctcctgtt ctgctgaccg taatatcaaa gcgaagatca accgtcaggg gatctggatc
    25021 gaaaaactgg aacataatcc aggcaaatat atcccggaag agctgcgcaa agcgggagaa
    25081 ggcgaagcgg tgcgcgttga ccttaaccgt ccgatgaaag agatcctcgc acagttgtcg
    25141 cagtatcccg tttctacacg cttatcgctt aacggcacga ttatcgtcgg tcgtgatatt
    25201 gctcacgcca aactgaaaga gcggatggat aacggtgaag ggctgccgca gtacatcaaa
    25261 gatcatccga tttactacgc gggtccggcc aaaacgccgg aaggttatgc ctccggttct
    25321 cttggcccaa cgaccgccgg acggatggat tcttatgtcg atcaactgca agcgctgggc
    25381 ggaagtatga tcatgctggc gaaaggcaac cgcagccagc aggtgacgga tgcctgtaaa
    25441 aaacacggtg gcttctacct tggcagtatc ggtggtccgg ccgctgtatt ggcgcaggga
    25501 agtattaaga gcctggaatg tgttgaatat ccggaactgg gaatggaagc catctggaaa
    25561 attgaagtgg aagatttccc ggcgtttatc cttgtggatg ataaaggaaa tgacttcttc
    25621 cagcagatac aactcacaca atgcacccgc tgtgtgaaat aaacagagcc gcccttcggg
    25681 gcggtttttt tacatggcac gaaagaccaa acatttgtta tcaaatagta aataatgagt
    25741 gagctaaaag ttacttaacg aaagcaaaac agaaagaaaa aattaatcag gtgaggagta
    25801 ggccatgaat acagtacgca gcgaaaaaga ttcgatgggg gcgattgatg tcccggcaga
    25861 taagctgtgg ggcgcacaaa ctcaacgctc gctggagcat ttccgcattt cgacggagaa
    25921 aatgcccacc tcactgattc atgcgctggc gctaaccaag cgcgcagcgg caaaagttaa
    25981 tgaagattta ggcttgttgt ctgaagagaa agcgagcgcc attcggcagg cggcggatga
    26041 agtactggca ggacagcatg acgacgaatt cccgctggct atctggcaga ccggctccgg
    26101 cacgcaaagt aacatgaaca tgaatgaagt gctggctaac cgggccagtg aattactcgg
    26161 cggcgtgcgc gggatggaac gtaaagttca ccctaacgac gacgtgaaca aaagccaaag
    26221 ttccaacgat gtctttccga cggcgatgca cgttgcggcg ctgctggcgc tgcgcaagca
    26281 actcattccg cagcttaaaa ccctgacaca gacgctgagt gaaaaatcgc gtgcatttgc
    26341 cgatatcgta aaaatcggtc gaacccactt gcaggacgcc accccgctaa cactggggca
    26401 ggagatttcc ggctgggtag cgatgctcga gcataatctc aaacatatcg aatacagcct
    26461 gcctcacgta gcggaactgg ctcttggcgg tacagcggtg ggtactggac taaataccca
    26521 tccggagtat gcgcgtcgcg tagcagatga actggcagtc attacctgtg caccgtttgt
    26581 taccgcgccg aacaaatttg aagcgctggc gacctgtgat gccctggttc aggcgcacgg
    26641 cgcgttgaaa gggttggctg cgtcactgat gaaaatcgcc aatgatgtcc gctggctggc
    26701 ctctggcccg cgctgcggaa ttggtgaaat ctcaatcccg gaaaatgagc cgggcagctc
    26761 aatcatgccg gggaaagtga acccaacaca gtgtgaggca ttaaccatgc tctgctgtca
    26821 ggtgatgggg aacgacgtgg cgatcaacat ggggggcgct tccggtaact ttgaactgaa
    26881 cgtcttccgt ccaatggtga tccacaattt cctgcaatcg gtgcgcttgc tggcagatgg
    26941 catggaaagt tttaacaaac actgcgcagt gggtattgaa ccgaatcgtg agcgaatcaa
    27001 tcaattactc aatgaatcgc tgatgctggt gactgcgctt aacacccaca ttggttatga
    27061 caaagccgcg gagatcgcca aaaaagcgca taaagaaggg ctgaccttaa aagctgcggc
    27121 ccttgcgctg gggtatctta gcgaagccga gtttgacagc tgggtacggc cagaacagat
    27181 ggtcggcagt atgaaagccg ggcgttaatc tgcaacatac aggtgcagcc gtggaatgat
    27241 caaacgaagc ggctgcgcct gaggtttata acggtgctgc acattgtcgg catcgtaatt
    27301 taacaactca ccaacgtccg gcacgcccgc accattatcc cgattaatca gtgcaatcag
    27361 cggtgtaggg caggcgtgtt ggacttgttt ttgatctcct ttgtaccaga cgcgggcaat
    27421 cggctgcacc ttcaccggac gtttgatttt taacttcgca ttctgtggca gggcagcgat
    27481 atcctgatac tctcgctcca gttttctttg ccactcctcg cgcgtccacg gtgcgacact
    27541 gcgtggtgat ttcaggcttt tttccagctg tgccaggact tcatcacgat gcaaattctt
    27601 aatgatatgt ttattagccc aaccaaagcg taaagtggcg ggatcgtgca gaacggtgag
    27661 cgtgcggtaa gcattaaggg tgatcagccc cggcaaatga cgatgcaccc attcaaaacg
    27721 tgccgcggtg gggagttctg attcaaccgt gacgatatgc tcgaacgtgg tcttgagttt
    27781 attgatgtgc tgaatatgac tgaccaacgc tgcttgcgaa aggttatcga cctggtaaca
    27841 caacacgcca ggcagacgga cagctgcttt gctgctgcga ttttcggact gttgttgaat
    27901 aaataaatga cggaaatgac gcaacgcctg cgactgcgcg tcgttgccga gatgttgttt
    27961 cacctcaata cgattaagcg gattatgctc atcctctttt tttacctccg gcaaagagaa
    28021 cacgcgggca accaatagct tgtgttgctc aagatgagcg gcaaatgcag ccagctcttg
    28081 ttccatctgg cgaaaagtag tgttgagtcg gtctacgaga tcgtaacgcg ccataatatt
    28141 aaccacttta gttacaacat ccttaattta tagcacagtg gtggtgactt gaccaccgtg
    28201 cgcgtagtgt caagcagagg taaattgcgg gatgttatgc cataacggcc agctaaacga
    28261 gaagcgggca ccacccagtt cgctggtgtc acaattaacc gtaccgccca ttgccagtgc
    28321 tatagagtgg acaattgcca gccccagccc gcagccgccg gttgagcgat cccggctggg
    28381 atcgaggcga acaaaaggtt caaagatatg ttcgcggttt tctggggcaa tccctgggcc
    28441 atcatcctca acaattaatg tcgctctatt ccccgacagt agcaggctgg tttcaaccgt
    28501 tgaatggcag tagcgcaggg cattattgag caaattatcc agcacgcgct ccattaagcg
    28561 catatccaac gccgcataat ggccttgcat gagcgttttt atccgtaccg ttttatcggg
    28621 cgttactgcc tgaatatctg ccagatgcgt tgacagccac aacggcaggt ctggttcgct
    28681 aagatgaagc tcgttttgtg ggcgatcgag tcgggcataa gtcagcagct cttcaattaa
    28741 agcttcaagt tgactgatat cacgattcaa cgcctgggat tcggcggcgc tcaggttatc
    28801 gctcatctcc agtcgataac gcaggcgcac taacggtgtt cgcagttcgt gagcgatacc
    28861 gtcaataagc tgttttttgc tggcaatcaa ggcgttgata ttgtccgcca tctggttaaa
    28921 tgcgatacca agtcgttcaa agctcgaacc ctcatcaaag tggatacgtt cattgagatg
    28981 cccatcgcca aatcgttgcg ccgctgcttc cagttttaac atatcctgcc agtgcggacg
    29041 catccagata aacaccggaa aggcgaggga aatagcaata aaagcgatca gggcgatatc
    29101 cagcaatcgc atctgatgga gataataaag ataaggaaca ggaccaactg ccagcacgta
    29161 gtggctgcgc gggatacgct gcaaaaaagt gtactgatcg tccagggcga caatttcgcc
    29221 gccacgcagc cggtgcatgg aaatatcatc aagatggtat ttactcagtg gctcgactcg
    29281 cagatcgaaa gagagattta aatccatctc tttcagcgtc ttaccccagt catgtggggg
    29341 gatctcacgc aattcgctgc gcatcagata cagcgaactg ttcatcaaat catccagcga
    29401 ctgtttgccc gcgcgttcgg cggtaaattt gtacaccaac ccaaccagca gagacatcac
    29461 aaggaagcag acaaacaata acaggtaaaa ctggataaac agttttttca tcgcttattc
    29521 ccatgcatga ggcgcaaaaa gatagccttt gttacgcaca gttttaatgc gataaggttc
    29581 agcggcgtta tcgagcagtt tttttcttaa ccgcgaaata gccacgtcca cgctacgatc
    29641 caggccgtca taactgacgc cgcgtaaatt tttcagcaat gcatcgcggt ccatgatttg
    29701 cccggcatgg gtagctaatt cccacaataa ttcgaaatca gctgtcgaga gcgagatttc
    29761 agtgttagcc agggtgacta cgcggttgat aggatcgatg gtcaacgtgc cgaaatgcag
    29821 ggctttgtag ggagtcagtg acgtttcctg aagacctttg gtcagcgtgg cttgctcatt
    29881 ctgacgcaaa tgcaaacgta aacgcgctaa caaaacagca gggggcgtcg ttttgagaat
    29941 atagtcgcag gcacccattt ccagtgccag gatgtggttc atatcgctat cgagagaggt
    30001 tagaagaaca atcggtccgg accactttgc gcgtaaatca cgacaaatgg tcatgccgtc
    30061 cttgcctggt agcatgatgt cgagtaacac caaatccgga ttttctcgca aaatggtttc
    30121 ttcggcctgg tcgccgcgcg gctctacggt aacctgtata tcatgttttg ccaggtacgc
    30181 ggcaatcagt gaaccgactt ccgcatcatc ttccacaaat acgatagtgt tcataacatt
    30241 cacggtagat ataaaaacgt cacaatacac cgcgctgttt ttacttacta tcaatcgttt
    30301 ctaaacaagt tttcatcagg tattctactg atgacttgtt atggaagtgt taaggtaaaa
    30361 agatggggct ggtaatcaaa gccgcgctcg gtgcgctggt agtgctgttg attggtgttt
    30421 tagcaaaaac gaaaaattat tatatcgccg ggctgattcc acttttcccg acctttgcgc
    30481 ttatcgcgca ttatattgtt gccagcgaac gcggcattga agccttacgc gcaaccatca
    30541 tttttagtat gtggtcgatt attccctatt ttgtttacct ggtgtcgctg tggtatttca
    30601 ccggaatgat gcgcttgccc gccgcttttg ttggttctgt tgcgtgttgg gggataagcg
    30661 catgggtgtt gattatatgc tggataaagc tgcattaacg cagatgacga ccgccatcaa
    30721 gtgggaaact gcgtccggtg acaaagcaac tggtaagtaa gtaatcgacc aggtcgatca
    30781 cttctttctc gccaggcgcg gttttcatca atgatttatt cagcgcctgt tgtcgatatt
    30841 cggcatcatc atgttcatta aacaggatca gcgatggcgc aatagaattc actttcactt
    30901 ccggtgccag cttgcgggca aacgagcggg tcatattatc cagtgccgct ttgcttgcag
    30961 catacgcaat atgtttgtcg ctaccgcgct ccaccacata atcggtaaag tgaatgatat
    31021 cgctggcggc gtgtccgtgc ccacgcagta atctttccag cgcatggttg agcaggtatg
    31081 gggtattaac gtggatctgc atcatgcaag ccagtacgtc ggccagtggc gcacccggtt
    31141 tttccgccat ccacgcactg gcgttatgca aaatagcacg cagaccatgg gtgcttttta
    31201 gtacttcatc ggcaaacgcc atcacaccgt cgttggtcga aaaatcagcc tgaatacact
    31261 gcgcacctgc attaatcagt ccatcaatgg ctggatagtg tgtccgatag ctgacaatca
    31321 ccggttgctt ttgattaatg aaatgccatg cgagggcgag gccgatgcga cgacctccgc
    31381 cagtaattaa tattggcaag ggctgggttt tacccatcgt tatctccttt gctatccaac
    31441 gatgggatga acgttatccc accagcatcc aggtcgccgg aactgaggca attaacagca
    31501 tgccaatcag caccatttcc tgacggttca gtacattatc atgtgtatgc gttttgcgcg
    31561 catacaggaa aaccagcagc cccggtgcat acagaacaac ggataacagc aagtgcattg
    31621 gaccagaggc gtacaataac cataagccat aaatgcaggc accgacacct accgctttat
    31681 gcagtggacg ggttgcgatt ttcagcaaga acgcgccgac caggaaatag ggcaccagaa
    31741 tcatttctga ggcgatggtc agcaacgtgt tgtaatcgga accggtcagc cagatgagta
    31801 ccagacaaat ttgcacgcag atgtttgtca accacagtga ggcggatggc gcagcttgtg
    31861 cgttctggcg cgcaaaaatg cgtgggaatg ctttatgagt ggcagccagg aacggaactt
    31921 ctgccgccat gatggtccag cttaagtacg cgccgcaaac ggaaacgatc aaaccggcag
    31981 cgatgatgat ttcgccccac ggtcccatca tttccaccat cagaccggcc attgacgggt
    32041 tacgaatttc agccagttca ggacgtgcca ccactcccag cgaaagcagc gttaccagca
    32101 agtaaacgcc cagagcggag agaaccgcca gcagtgtcgc tttgccaaca tcacgtttat
    32161 tacgcgcacg cgcagaaaca accacggcac cttccacacc aatgaaaacc cacagggtga
    32221 tcagcatggt gtttttcacc tgttcccaaa caggtacgcc aagtgcaagt ccggtgaagt
    32281 cgagcttgaa gatatccagt ttgaacatca tcatcgccag cacaacaaac agacccagcg
    32341 gcaacaattt tgccagtgtc gccaccaggt taatgctggc agcggtttgc accccgcgca
    32401 ggatcaaaaa gtgaacaatc cacaataaag ccgatgcacc gactatcgat tgccaggtat
    32461 taccatcgcc aaacaggcgc aattccggcg tgtccgtaaa aaagcttaac gcggaaaaaa
    32521 cgatcaccag ataggagacg ttggcgatga ctgcgcacag ccagtatccc catgcggaac
    32581 aaaagccgat tagctcgcca aacccttcgc gggcataggt aaagataccg ccgtcaagtt
    32641 cgggacgaat gcgcgtgagg atcagcatgg caaaggccag caataaaatg ccagcgccag
    32701 taatacccca gccgatgagc agtgctgccg ggctggcaac tgccgccata ttttgcggca
    32761 gactgaaaac acccgcgccc agcattgagc ttaataccag cgcggtgagt gcgctcagtc
    32821 ccagtttctt ttccatcgat atccagtgat taaaacgtga taagaagaat aaatattagg
    32881 gcaaaacgca taaaaatgga gtttgccact gaagcgcggg attttacgaa gagatgtgtc
    32941 tgcatgcaat ggtcgcgggg aaatttgtga ctaaaaatgc gaaaggcggc atcaagccgc
    33001 cttataagag taactgaata gttatttata cagatctgca ctgacggtca ggttattacc
    33061 acgttcctgc cactggcggg tgatgtggta atatttagca cctttcttcg cggctcgttt
    33121 cgcaacctga taggagactt cggtcatgtt gccgtagttg ccagagaatt tgatgctgtc
    33181 gaacgggacc atcatcgctg ctgtcgcttt gttcagttct tcgactttag tgccatccgg
    33241 aagcgtgaca gtgtaacgcc cgccttttga tgactgggtt tcaaagaagc gaccgacttc
    33301 agaacttggt gatgcggtag tcgcaacacc cgggatctca actttcttcg cggcttcgcc
    33361 accggcagcc agagctgcac gtcctgcttc ggaatctgcc gggatcacat ccgggctctg
    33421 gacgatacgt ttcttagcat cttttttata gatgaatgca gtaatacgct ggttgccgcc
    33481 ctggttggca tcgatttgac gaacgatgta gaaagagtag gcacctttcg cttttgccgc
    33541 tttggtgatg gcatcattga cttctggctg gctacgatag aagccctgga cggtgaccgt
    33601 gtcaaacggt tcaatcagaa cagcctgatc tttcggcagt tcgacaacac cgttaattac
    33661 gcgattactt gtttcttctg ctttttcagc atcggcttta tagaggtcag cgaccacacg
    33721 ccagttaccg ctgttcccaa aatcagaagt gtcgacaaca taaaaagagg cggcaccttc
    33781 tttatctgcg cgacgagaaa cggctttcac cgcttcgcca atagcattaa aacgaccggt
    33841 aaccactaca cggtcaaaag gtttaaccgc tgccgcttgc tccggtgtca gttctgttgc
    33901 tgcgttaacg gagaatgccg tagcagaaag cagtgccgac gccaggaggg tgttcttaag
    33961 cttcataaaa ataatccttc gccttgcgca aaccaggtac tggtattgtt attaacgaga
    34021 aacgtggctg attattgcat ttaaacggtg taactgtctg cgtcattttt catatcacat
    34081 tccttaagcc aattttaatc ctgctcaaat gaccgtctat gcttaaaaaa cagccgtatc
    34141 agcatcatta ctactgaagc aactgaattg tataagttaa tttaatgtta agtagtgatt
    34201 cgtgccgggg cgatgtctcg ttttacccga ccgtcgaaga caattatcag tctttatccg
    34261 gcgttctaag gtgtttatcc cactatcacg gctgaatcgt taatattttg cgagttcacg
    34321 ccgaaatact gatttttggc gctagatcac aggcataatt ttcagtacgt tatagggcgt
    34381 ttgttactaa tttattttaa cggagtaaca tttagctcgt acatgagcgg cttgtgtggc
    34441 tcctgacaca ggcaaaccat catcaataaa accgatggaa gggaatatca tgcgaattgg
    34501 cataccaaga gaacggttaa ccaatgaacc ccgtgttgca gcaacgccaa aaacagtgga
    34561 acagctgctg aaactggggt ttaccgtcgc ggtagagagc ggcgcgggtc aactggcaag
    34621 ttttgacgat aaagcgtttg tgcaagcggg cgctgaaatt gtagaaggga atagcgtctg
    34681 gcagtcagag atcattctga aggtcaatgc gccgttagat gatgaaattg cgttactgaa
    34741 tcctgggaca acgctggtga gttttatctg gcctgcgcag aatccggaat taatgcaaaa
    34801 acttgcggaa cgtaacgtga ccgtgatggc gatggactct gtgccgcgta tctcacgcgc
    34861 acaatcgctg gacgcactaa gctcgatggc gaatatcgcc ggttatcgcg ccattgttga
    34921 agcggcacat gaatttgggt gcttctttac cgggcaaatt actgcggccg ggaaagtgcc
    34981 accggcaaaa gtgatggtga ttggtgcggg tgttgcaggt ctggccgcca ttggcgcagc
    35041 aaacagtctc ggcgcgattg tgcgtgcatt cgacacccgc ccggaagtga aagaacaagt
    35101 tcaaagtatg ggcgcggaat tcctcgagct ggattttaaa gaggaagcgg gcagcggcga
    35161 tggctatgcc aaagtgatgt cggacgcgtt catcaaagcg gaaatggaac tctttgccgc
    35221 ccaggcaaaa gaggtcgata tcattgtcac caccgcgctt attccaggca aaccagcgcc
    35281 gaagctaatt acccgtgaaa tggttgactc catgaaggcg ggcagtgtga ttgtcgacct
    35341 ggcagcccaa aacggcggca actgtgaata caccgtgccg ggtgaaatct tcactacgga
    35401 aaatggtgtc aaagtgattg gctataccga tcttccgggc cgtctgccga cgcaatcctc
    35461 acagctttac ggcactaacc tcgttaatct gctgaaactg ttgtgcaaag agaaaaacgg
    35521 taacatcact gttgattttg atgatgtggt gattcgcggc gtgactgtga tccgtgcggg
    35581 cgaaattacc tggccggcac cgccgattca ggtatcagct cagccgcagg tggcacaaaa
    35641 agcggcaccg gaagtgaaaa ctgaggaaaa atgtgcctgt tcaccgtggc gtaaatacgc
    35701 gttgatggcg ctggcaatca tccttttcgg ctggctggca agcgttgcgc cgaaagagtt
    35761 tcttgggcac ttcaccgttt tcgcgctggc ctgcgttgtc ggttattacg tggtgtggaa
    35821 cgtatcgcac gcgctgcata caccgttgat gtcggtcacc aacgcgattt cagggattat
    35881 tgttgtcgga gcactgttgc agattggcca gggcggctgg gttagcttcc ttagttttat
    35941 cgcggtgctt atagccagca ttaatatttt cggtggcttc accgtgactc agcgcatgct
    36001 gaaaatgttc cgcaaaaatt aaggggtaac atatgtctgg aggattagtt acagctgcat
    36061 acattgttgc cgcgatcctg tttatcttca gtctggccgg gctttcgaaa catgaaacgt
    36121 ctcgccaggg taacaacttc ggtatcgccg ggatggcgat tgcgctaatc gccaccattt
    36181 ttggaccgga tacgggtaac gttggctgga tcttgctggc gatggtcatt ggtggggcaa
    36241 ttggtatccg tctggcgaag aaagttgaaa tgaccgaaat gccagaactg gtggcgatcc
    36301 tgcatagctt cgtgggtctg gcggcagtgc tggttggctt taacagctat ctgcatcatg
    36361 acgcgggaat ggcaccgatt ctggtcaata ttcacctgac ggaagtgttt ctcggtatct
    36421 tcatcggggc ggtaacgttc acgggttcgg tggtggcgtt cggcaaactg tgtggcaaaa
    36481 tttcgtctaa accgttgatg ctgccaaacc gtcacaaaat gaacctggca gctctggtcg
    36541 tttccttcct gctgctgatt gtatttgttc gcacggatag cgtcggcctg caagtgctgg
    36601 cattgctaat aatgaccgcc attgcgctgg tatttggctg gcatttagtc gcctccatcg
    36661 gtggtgcaga tatgccggtg gtggtgtcga tgctgaactc gtactccggc tgggcggctg
    36721 cggctgcggg ctttatgctc agcaacgacc tgctgattgt gaccggtgcg ctggtcggtt
    36781 cctcgggggc tatcctttct tacattatgt gtaaggcgat gaaccgttcc tttatcagcg
    36841 ttattgcggg tggtttcggc accgacggct cttctactgg cgatgatcag gaagtgggtg
    36901 agcaccgcga aatcaccgca gaagagacag cggaactgtt gaaaaactcc cattcagtga
    36961 tcattactcc ggggtacggc atggcagtcg cgcaggcgca atatcctgtc gctgaaatta
    37021 ccgagaaact gcgcgctcgt ggtatcaacg tgcgtttcgg tatccacccg gttgcggggc
    37081 gtttgcctgg acatatgaac gtattgctgg ctgaagcaaa agtaccgtat gacatcgtgc
    37141 tggaaatgga cgagatcaac gatgactttg ctgataccga taccgtactg gtgattggtg
    37201 ctaacgatac ggttaacccg gcggcgcagg atgatccgaa gagtccgatt gctggtatgc
    37261 ctgtgctgga agtgtggaaa gcgcagaacg tgattgtctt taaacgttcg atgaacactg
    37321 gctatgctgg tgtgcaaaac ccgctgttct tcaaggaaaa cacccacatg ctgtttggtg
    37381 acgccaaagc cagcgtggat gcaatcctga aagctctgta accctgacgg cctctgcgga
    37441 ggccgtcact ctttattgag atcgcttaac agaacggcga tgctttgacc tcccgctgtt
    37501 tgttcaagcg caattttgac aataattgtt aacggcacgg aaagcagcat acccaccggt
    37561 cctaacaacc atccccaaaa aatcaacgac aaaaatacca ccaatgtgga aagtcccagc
    37621 ccacgcccca tgatgcgtgg ctccagaata ttgccgaaga ccagattaat cagcagatat
    37681 cccgccagca ccaacaacgc ttcgtagaag ccattaaaca ccagtacctg agcaataggg
    37741 gggattgccg cgagaactga accaatatta gggatgtaat taagcgcaaa ggccagcaat
    37801 ccccagacaa aagcgaagcg aacatcgagg gcggcgagca tcgcccatgc gaccaggccg
    37861 gtgatgatgc tgatggctgt tttcagcacc agataatgag acacactgtc aatcgcacgc
    37921 tgaatcgccg ccatcccttc aaccggacgc gccatcattt gcttaaattt tccgggcaat
    37981 tgtggcactt cgagcagcat aaacagcacc gtcagcagca ataaaaatat tgatgacatg
    38041 gcattagata actgcgtcaa taagttggtg agcaacgtca tcgccgcgtt cggatcaatg
    38101 taatgcgcca gttggtcaac tgagacgtca atccctacgc gttgcaacaa cggctcaaga
    38161 gcttgcagcg gcgtcataat agagttgcga tattgcggta acgtccgcgt caactcgttg
    38221 agcgtggaac ccagataagc taacagcaac accatagcca tcacgatgat ggtcatcaaa
    38281 atcgacactg ccagtacacg cggcacacgc cagcggacca tgtgttgcac cagcgggtta
    38341 agaataacag caataaataa tgcgagaata aacggcacga tgatctcggc ggcaaaacgg
    38401 ataccgcaga gaataatgac cagcattccc aacataatga cgatttttag gccattgagc
    38461 gtgatgagcg gctttgccat gcttgtcccc gttttttctt cctttgctaa taataatgtc
    38521 tttttcgctc cgtcatcata acgaaaatca aaccgctcag gtaaagaagt gaaaatattt
    38581 tgagttaatt cttaagctat gatacaaatc aggcgtgttc aactaccgag gacaattatc
    38641 atccgcgatg acgagaagca acactgcgga taattgtaat attatggaca atttgttcag
    38701 gacgttattt tctacattta cccatcttcg tacctcatcc actatcttgc tcgtaggtga
    38761 gcagcactgg cgcaacgcgc tttagtcctt tcttctgcct aagcttgccc tatacgcggg
    38821 gcttgaccac tttactttgg tttcgctgaa ttaagcgaaa attaaaataa ttctcttgca
    38881 gg
//
LOCUS       DBFIFM010000017        37775 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.017, whole genome shotgun sequence.
ACCESSION   DBFIFM010000017 DBFIFM010000000
VERSION     DBFIFM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.017"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            161..1729
                     /gene="cydA"
                     /locus_tag="N7C74_000823"
     CDS             161..1729
                     /gene="cydA"
                     /locus_tag="N7C74_000823"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HGU2430755.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1745..2884
                     /gene="cydB"
                     /locus_tag="N7C74_000824"
     CDS             1745..2884
                     /gene="cydB"
                     /locus_tag="N7C74_000824"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HGU2430756.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            2899..3012
                     /gene="cydX"
                     /locus_tag="N7C74_000825"
     CDS             2899..3012
                     /gene="cydX"
                     /locus_tag="N7C74_000825"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HGU2430757.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3012..3305
                     /gene="ybgE"
                     /locus_tag="N7C74_000826"
     CDS             3012..3305
                     /gene="ybgE"
                     /locus_tag="N7C74_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HGU2430758.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3455..3859
                     /gene="ybgC"
                     /locus_tag="N7C74_000827"
     CDS             3455..3859
                     /gene="ybgC"
                     /locus_tag="N7C74_000827"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HGU2430759.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            3856..4548
                     /gene="tolQ"
                     /locus_tag="N7C74_000828"
     CDS             3856..4548
                     /gene="tolQ"
                     /locus_tag="N7C74_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HGU2430760.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4552..4980
                     /gene="tolR"
                     /locus_tag="N7C74_000829"
     CDS             4552..4980
                     /gene="tolR"
                     /locus_tag="N7C74_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HGU2430761.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5045..6319
                     /gene="tolA"
                     /locus_tag="N7C74_000830"
     CDS             5045..6319
                     /gene="tolA"
                     /locus_tag="N7C74_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HGU2430762.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADDKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKKAAEKAAAEKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADK
                     KAAAAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNG
                     ASGADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQ
                     AALAAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            6452..7744
                     /gene="tolB"
                     /locus_tag="N7C74_000831"
     CDS             6452..7744
                     /gene="tolB"
                     /locus_tag="N7C74_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HGU2430763.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            7779..8300
                     /gene="pal"
                     /locus_tag="N7C74_000832"
     CDS             7779..8300
                     /gene="pal"
                     /locus_tag="N7C74_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HGU2430764.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8310..9101
                     /gene="cpoB"
                     /locus_tag="N7C74_000833"
     CDS             8310..9101
                     /gene="cpoB"
                     /locus_tag="N7C74_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HGU2430765.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVIQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9266..9341
                     /locus_tag="N7C74_000834"
     tRNA            9266..9341
                     /locus_tag="N7C74_000834"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9299..9301,aa:Lys,seq:ttt)
     gene            9377..9452
                     /locus_tag="N7C74_000835"
     tRNA            9377..9452
                     /locus_tag="N7C74_000835"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9410..9412,aa:Val,seq:tac)
     gene            9455..9530
                     /locus_tag="N7C74_000836"
     tRNA            9455..9530
                     /locus_tag="N7C74_000836"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9488..9490,aa:Lys,seq:ttt)
     gene            9582..9657
                     /locus_tag="N7C74_000837"
     tRNA            9582..9657
                     /locus_tag="N7C74_000837"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9615..9617,aa:Val,seq:tac)
     gene            9661..9736
                     /locus_tag="N7C74_000838"
     tRNA            9661..9736
                     /locus_tag="N7C74_000838"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9694..9696,aa:Lys,seq:ttt)
     gene            9883..9958
                     /locus_tag="N7C74_000839"
     tRNA            9883..9958
                     /locus_tag="N7C74_000839"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9916..9918,aa:Lys,seq:ttt)
     gene            10236..11279
                     /gene="nadA"
                     /locus_tag="N7C74_000840"
     CDS             10236..11279
                     /gene="nadA"
                     /locus_tag="N7C74_000840"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HGU2430766.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKKTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNYEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            11317..12036
                     /gene="pnuC"
                     /locus_tag="N7C74_000841"
     CDS             11317..12036
                     /gene="pnuC"
                     /locus_tag="N7C74_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HGU2430767.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVIIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(12033..12974)
                     /gene="zitB"
                     /locus_tag="N7C74_000842"
     CDS             complement(12033..12974)
                     /gene="zitB"
                     /locus_tag="N7C74_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HGU2430768.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(13088..13468)
                     /gene="ybgS"
                     /locus_tag="N7C74_000843"
     CDS             complement(13088..13468)
                     /gene="ybgS"
                     /locus_tag="N7C74_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HGU2430769.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            13784..14836
                     /gene="aroG"
                     /locus_tag="N7C74_000844"
     CDS             13784..14836
                     /gene="aroG"
                     /locus_tag="N7C74_000844"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HGU2430770.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(15002..15754)
                     /gene="gpmA"
                     /locus_tag="N7C74_000845"
     CDS             complement(15002..15754)
                     /gene="gpmA"
                     /locus_tag="N7C74_000845"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HGU2430771.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(15956..16996)
                     /gene="galM"
                     /locus_tag="N7C74_000846"
     CDS             complement(15956..16996)
                     /gene="galM"
                     /locus_tag="N7C74_000846"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HGU2430772.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGKQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNYLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLHPGEE
                     YSSLTEYQFIAE"
     gene            complement(16990..18138)
                     /gene="galK"
                     /locus_tag="N7C74_000847"
     CDS             complement(16990..18138)
                     /gene="galK"
                     /locus_tag="N7C74_000847"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HGU2430773.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(18142..19188)
                     /gene="galT"
                     /locus_tag="N7C74_000848"
     CDS             complement(18142..19188)
                     /gene="galT"
                     /locus_tag="N7C74_000848"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HGU2430774.1"
                     /translation="MTQFNPVDHPHRRYNQLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(19198..20214)
                     /gene="galE"
                     /locus_tag="N7C74_000849"
     CDS             complement(19198..20214)
                     /gene="galE"
                     /locus_tag="N7C74_000849"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGU2430775.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SRACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(20476..21948)
                     /gene="modF"
                     /locus_tag="N7C74_000850"
     CDS             complement(20476..21948)
                     /gene="modF"
                     /locus_tag="N7C74_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HGU2430776.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITPLLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYHYALTKIN"
     gene            complement(22016..22804)
                     /gene="modE"
                     /locus_tag="N7C74_000851"
     CDS             complement(22016..22804)
                     /gene="modE"
                     /locus_tag="N7C74_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HGU2430777.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            22933..23082
                     /gene="acrZ"
                     /locus_tag="N7C74_000852"
     CDS             22933..23082
                     /gene="acrZ"
                     /locus_tag="N7C74_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HGU2430778.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            23249..24022
                     /gene="modA"
                     /locus_tag="N7C74_000853"
     CDS             23249..24022
                     /gene="modA"
                     /locus_tag="N7C74_000853"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU2430779.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            24022..24711
                     /gene="modB"
                     /locus_tag="N7C74_000854"
     CDS             24022..24711
                     /gene="modB"
                     /locus_tag="N7C74_000854"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HGU2430780.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            24714..25772
                     /gene="modC"
                     /locus_tag="N7C74_000855"
     CDS             24714..25772
                     /gene="modC"
                     /locus_tag="N7C74_000855"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HGU2430781.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(25773..26591)
                     /gene="ybhA"
                     /locus_tag="N7C74_000856"
     CDS             complement(25773..26591)
                     /gene="ybhA"
                     /locus_tag="N7C74_000856"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HGU2430782.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDEMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            26746..27741
                     /gene="pgl"
                     /locus_tag="N7C74_000857"
     CDS             26746..27741
                     /gene="pgl"
                     /locus_tag="N7C74_000857"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HGU2430783.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNIDHRGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(27782..28735)
                     /gene="ybhD"
                     /locus_tag="N7C74_000858"
     CDS             complement(27782..28735)
                     /gene="ybhD"
                     /locus_tag="N7C74_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2430784.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            28919..29971
                     /gene="ybhH"
                     /locus_tag="N7C74_000859"
     CDS             28919..29971
                     /gene="ybhH"
                     /locus_tag="N7C74_000859"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HGU2430785.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEKRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGGLDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            30047..31480
                     /gene="ybhI"
                     /locus_tag="N7C74_000860"
     CDS             30047..31480
                     /gene="ybhI"
                     /locus_tag="N7C74_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HGU2430786.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESSVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            31663..33924
                     /gene="ybhJ"
                     /locus_tag="N7C74_000861"
     CDS             31663..33924
                     /gene="ybhJ"
                     /locus_tag="N7C74_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HGU2430787.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGAAQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGSSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     VEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(34065..35348)
                     /gene="ybhC"
                     /locus_tag="N7C74_000862"
     CDS             complement(34065..35348)
                     /gene="ybhC"
                     /locus_tag="N7C74_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HGU2430788.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWGDAVISNRPFAGNTGSVD
                     DSDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(35483..36553)
                     /locus_tag="N7C74_000863"
     CDS             complement(35483..36553)
                     /locus_tag="N7C74_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2430789.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(36531..36749)
                     /locus_tag="N7C74_000864"
     CDS             complement(36531..36749)
                     /locus_tag="N7C74_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU2430790.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(36789..36956)
                     /locus_tag="N7C74_000865"
     CDS             complement(36789..36956)
                     /locus_tag="N7C74_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2430791.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADITNI
                     RIEELKEHQAA"
     gene            37199..>37775
                     /locus_tag="N7C74_000866"
     CDS             37199..>37775
                     /locus_tag="N7C74_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2430792.1"
                     /translation="MSYFLRKKWMVNLSGAGKILWALNMKKDSYPYLICMTVLGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGMCIFAGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYA"
BASE COUNT         9061 a         9187 c         9865 g         9662 t
ORIGIN      
        1 attcattaac atatttgtaa cttttttatc attgtctttt cctcttttga tgaaatggca
       61 acgatagagg cgagcaaata attttgtatt ggggcatgcg tgtgaccctt tctaacgggg
      121 ttcactctcg gagtcttcat gcgatgagca aggagtcatg atgttagata tagtcgaact
      181 gtcgcgctta cagtttgcct tgaccgcgat gtaccacttc ctttttgtgc cactgacgct
      241 cggtatggcg ttcctgctgg ccattatgga aacggtctac gtcctttccg gcaaacagat
      301 ttataaagat atgaccaagt tctggggcaa gttgtttggt atcaacttcg ctctgggtgt
      361 ggctaccggt ctgaccatgg agttccagtt cgggactaac tggtcttact attcccacta
      421 tgtaggggat atcttcggtg cgccgctggc aatcgaaggt ctgatggcct tcttcctcga
      481 atccaccttt gtaggtctgt tcttcttcgg ttgggatcgt ctgggtaaag ttcagcatat
      541 gtgtgtcacc tggctggtgg cgctcggttc aaacctgtcc gcactgtgga ttctggttgc
      601 gaacggctgg atgcaaaacc caatcgcgtc cgatttcaac tttgaaacta tgcgtatgga
      661 gatggtgagc ttctccgagc tggtgcttaa cccggttgct caggtgaaat tcgttcacac
      721 tgtagcgtct ggttatgtga ctggcgcgat gttcatcctc ggtatcagcg catggtatat
      781 gctgaaaggt cgtgacttcg ccttcgctaa acgctccttt gctatcgctg ccagcttcgg
      841 tatggctgcc gttctgtctg ttattgttct gggtgatgaa tccggctacg aaatgggcga
      901 cgtgcagaaa accaaactgg ctgctattga agctgagtgg gaaacgcaac ctgcgcctgc
      961 tgcctttact ctgttcggca ttcctgatca ggaagaggag acgaacaaat ttgcgatcca
     1021 gatcccttac gcgctgggca taattgcaac gcgttccgtg gatactccag ttatcggcct
     1081 gaaagaactg atggtgcagc atgaagaacg cattcgtaac gggatgaagg cgtactctct
     1141 gctcgaacaa ctgcgttctg gttctaccga ccaggcggtt cgtgaccagt tcaatagcat
     1201 gaagaaggac ctcggttacg gtctgctgct gaaacgctat acgccaaacg tggctgatgc
     1261 gactgaagcg cagattcaac aggcaaccaa agactccatt ccgcgtgtag cgccgctgta
     1321 cttcgcgttc cgtatcatgg tggcgtgtgg cttcctgctg ctggcaatca tcgcgctctc
     1381 tttctggagt gtcatccgca accgcattgg cgagaaaaaa tggctcctgc gcgccgcgct
     1441 gtacggtatt ccgctgccgt ggattgctgt agaagcgggc tggttcgtgg ctgaatatgg
     1501 ccgccaaccg tgggctatcg gtgaagtgct gccgacagct gtggcgaact cgtcactgac
     1561 cgcaggcgat ctcatcttct caatggtgct gatttgcggc ctgtataccc tgttcctggt
     1621 ggcagaattg ttcttaatgt tcaagtttgc acgcctcggc ccaagcagcc tgaaaaccgg
     1681 tcgctatcac tttgagcagt cttccacgac tactcagccg gcacgctaag acaggagtcg
     1741 tcaaatgatc gattatgaag tattgcgttt tatctggtgg ctgctggttg gcgttctgct
     1801 gattggtttt gcagtcactg atggtttcga catgggggtg ggcatgctca cccgtttcct
     1861 cggtcgtaac gacaccgagc gtcgaattat gattaactcc atcgcaccac actgggacgg
     1921 taaccaggtt tggctgatca ccgcaggcgg cgcactgttt gctgcctggc cgatggtcta
     1981 tgccgctgcg ttctccggct tctatgtggc gatgatcctc gtgctggcgt ctttgttctt
     2041 ccgtccggtc ggttttgact accgctccaa gattgaagaa acccgctggc gtaacatgtg
     2101 ggactggggc atcttcattg gtagcttcgt tccgccgttg gtgattggtg tagcgttcgg
     2161 taacctgttg cagggcgtac cgttcaacgt tgatgaatat ctgcgtctgt actacaccgg
     2221 taacttcttc cagctgctta acccgttcgg cctgctggca ggcgtggtga gcgtagggat
     2281 gatcattact cagggcgcaa cctatctgca aatgcgtacc gtgggcgaac tgcacctgcg
     2341 tacccgtgca acggctcagg tggcggcact ggtgacactg gtctgtttcg cactggctgg
     2401 cgtatgggtg atgtacggta tcgatggtta tgtcgtgaaa tcgacaatgg accattacgc
     2461 agcctctaac ccgctgaata aagaagtggt tcgtgaagct ggcgcatggc tggttaactt
     2521 caacaacacg ccaattctgt gggctattcc ggcactgggt gtggttctgc cgctgctgac
     2581 catcctgact gcacgtatgg ataaagccgc gtgggcgttt gtgttctcct ccctgacgct
     2641 ggcctgcatc atcctgactg ccggtatcgc aatgttcccg tttgtgatgc cgtccagcac
     2701 catgatgaac gcaagtctga caatgtggga tgcaacttcc agccagctga cacttaacgt
     2761 catgacctgg gttgcggtgg ttctggtacc gatcattctg ctctacaccg cctggtgtta
     2821 ctggaaaatg ttcggtcgta tcaccaaaga agatattgaa cgtaacaccc actctctgta
     2881 ctaagtaagg agctaaaaat gtggtatttc gcatggattc tgggaacgct tcttgcctgt
     2941 tcgtttgggg taatcaccgc gctggcgctt gagcacgtcg aatcaggcaa agccggtcaa
     3001 gaagacatct gatgagtaag attatcgcaa ccttgtatgc ggtaatggac aagcgccccc
     3061 tgcgggcgct ttccttcgtg atggcgcttc tgttagcagg atgtatgttt tgggacccat
     3121 ctcgtttcgc cgcgaagacc agtgaactgg aaatctggca tggtttattg ctgatgtggg
     3181 ccgtctgtgc tggtgtgatt cacggcgtgg gctttcgtcc gcagaaggtt ctttggcaag
     3241 ggattttttg cccattgctt gccgatattg ttctcattgt cgggctgatt ttcttcttct
     3301 tttaaatcag aattctcttt aaaaaattat gggccgtgcc aggcccataa atttttacgc
     3361 tcccttaact tgccctcatt cccaaacctc aatcgcgcgc gtatagtagc agcgtttaaa
     3421 agctctaact tttgttgcat taccgggatg taaagtgaat acaacgctgt ttcgatggcc
     3481 ggttcgcgtc tactatgaag ataccgatgc cggtggtgtg gtgtaccacg ccagttacgt
     3541 cgctttttat gaaagagcac gcacagagat gctgcgtcat catcacttca gtcaacaggc
     3601 gctgatggct gaacgcgttg cctttgtggt acgtaaaatg acggtggaat attacgcacc
     3661 tgcgcggctc gacgatatgc tcgaaataca gactgaaata acatcaatgc gtggcacctc
     3721 tttggttttc acgcaacgta ttgtcaacgc cgagaatact ttgctgaatg aagcagaggt
     3781 tctggttgtt tgcgttgacc cactcaaaat gaagcctcgt gcgcttccca agtctattgt
     3841 cgcggagttt aagcagtgac tgacatgaat atccttgatt tgttcctgaa ggctagcctt
     3901 ctggttaaac ttatcatgtt gattttgatt ggtttttcaa tcgcatcctg ggccattatt
     3961 atccagcgga cccgtattct taacgcagcg gcgcgcgaag ccgaagcgtt tgaagataaa
     4021 ttctggtctg gaatcgaact ctctcgcctc tatcaagaga gccaggggaa acgggataat
     4081 ctgactggtt cggaacaaat cttttacagc gggttcaaag aatttgtgcg cctgcatcgt
     4141 gccaatagcc atgcgccgga agccgtagtg gaaggggcgt cgcgtgcgat gcgtatttcc
     4201 atgaaccgcg aacttgaaaa tctggaaacg cacattccgt tcctcggtac ggttggctcc
     4261 atcagcccgt atattggtct gtttggtacg gtctggggga tcatgcacgc ctttatcgcc
     4321 ctcggggcgg taaaacaagc aacactgcaa atggttgcgc ccggtatcgc agaagcgttg
     4381 attgcgacgg caattggtct gttcgccgct attccggcgg taatggctta taaccgtctc
     4441 aaccaacgtg taaacaaact ggaactgaat tacgacaact ttatggaaga gtttaccgcg
     4501 attctgcacc gccaggcgtt taccgttagc gagagcaaca aggggtaagc catggccaga
     4561 gcgcgtggac gaggtcgtcg cgatctcaag tccgaaatca acattgtacc gttgctggac
     4621 gtactgctgg tgctgttgct gatctttatg gcgacagcgc ccatcatcac ccagagcgtg
     4681 gaggtcgatt tgccagacgc tactgaatca caggcggtga gcagtaacga taatccgcca
     4741 gtgattgttg aagtgtctgg tattggtcag tacaccgtgg tggttgagaa agatcgcctg
     4801 gagcgtttac caccagagca ggtggtggcg gaagtgtcca gccgtttcaa ggccaacccg
     4861 aaaacggtct ttctgatcgg tggcgcaaaa gatgtgcctt acgatgaaat aattaaagca
     4921 ctgaacttgt tacatagtgc gggtgtgaaa tcggttggtt taatgacgca gcctatctaa
     4981 acatctgcgt ttcccttgct tgaaagagag cgggtaacag gcgaacagtt tttggaaacc
     5041 gagagtgtca aaggcaaccg aacaaaacga caagctcaag cgggcgataa ttatttcagc
     5101 agtgctgcat gtcatcttat ttgcggcgct gatctggagt tcgttcgatg agaatataga
     5161 agcttcagct ggaggcggcg gtggttcgtc catcgacgct gtcatggttg attcaggtgc
     5221 ggtagttgag cagtacaaac gcatgcaaag ccaggaatca agcgcgaagc gttctgatga
     5281 gcagcgcaag atgaaggaac agcaggctgc tgaagaactg cgtgagaaac aagcggctga
     5341 acaggaacgc ctgaagcaac ttgagaaaga gcggttagct gctcaggaac agaaaaagca
     5401 ggctgaagaa gccgcaaaac aggccgagtt aaagcagaag caagcggaag aggcggcagc
     5461 gaaagcggcg gcagatgcta aagcgaaggc cgaagcggat gataaagctg cggaagaagc
     5521 agcgaagaaa gcggctgcag acgcgaagaa aaaagcagaa gcagaagccg ccaaagccgc
     5581 agccgaagcg cagaaaaaag ccgaggcagc agctgcggcg ctgaagaaga aagcggaagc
     5641 ggcagaagca gctgcagctg aagcaagaaa gaaagcggca gcagagaaag ctgcagccga
     5701 caaaaaagca gcagagaaag ctgcagccga caaaaaagca gcagaaaaag cggctgctga
     5761 aaaggcagca gcagagaaag ctgcagccga caaaaaagca gcagaaaaag cggctgctga
     5821 aaaggcagca gctgataaga aagcagcggc agaaaaagcc gccgcagaca aaaaagcggc
     5881 agctgcaaaa gcagcagctg aaaaagccgc tgcagcaaaa gctgccgcgg aggcagatga
     5941 tattttcggt gagctaagct ctggtaagaa tgcaccgaaa acggggggag gggcgaaagg
     6001 gaacaatgct tcgcctgccg ggagtggtaa tactaaaaac aatggcgcat caggggccga
     6061 tatcaataac tatgccgggc agattaaatc tgctatcgaa agtaagttct atgacgcatc
     6121 gtcctatgca ggcaaaacct gtacgctgcg cataaaactg gcacccgatg gtatgttact
     6181 ggatatcaaa cctgaaggtg gcgatcccgc actttgtcag gctgcgttgg cagcagctaa
     6241 acttgcgaag atcccgaaac caccaagcca ggcagtatat gaagtgttca aaaacgcgcc
     6301 attggacttc aaaccgtaat cgcgatgttg actgttccga cggtcaacat caggcaccgg
     6361 ttgccacggg gttctggtag ttttgtgtat tttagtttgt taacattctg ctaaattatc
     6421 gtgggccatc ggtccagata agggagatat gatgaagcag gcattacgag tagcatttgg
     6481 ttttctcata ctgtgggcat cagttctgca tgctgaagtc cgcattgtga tcgacagcgg
     6541 tgtagattcc ggtcgtccta ttggagttgt tcctttccag tgggcgggcc ctggtgcggc
     6601 acctgaagat attggcggca tcgttgctgc cgacttgcgt aacagcggta aatttaatcc
     6661 gttagatcgc gctcgtctgc cacagcagcc gggtagtgcg caggaagtac aaccagctgc
     6721 atggtccgcg ctgggtattg acgctgtagt tgtcggtcag gtcactccga atccggatgg
     6781 ttcttacaat gttgcttatc aacttgttga cactggcggc gcaccgggta ctgtacttgc
     6841 tcagaactcg tacaaagtga acaagcagtg gctgcgttat gctggtcata ccgccagtga
     6901 tgaagtgttt gaaaaactga ccggtattaa aggtgcgttc cgtacccgta ttgcctacgt
     6961 tgttcagacc aacggcggtc agttcccgta tgaactgcgc gtatctgact atgacggtta
     7021 caaccagttt gtcgttcacc gttcaccaca gccgctgatg tccccggcgt ggtcaccaga
     7081 cggttctaaa ctggcttatg tgaccttcga aagcggtcgt tccgcgctgg ttatacaaac
     7141 gctggcaaac ggcgctgtac gtcaggtggc ttcattcccg cgtcacaacg gtgcgcctgc
     7201 attctcgcca gacggcagca aactggcatt cgccttgtcg aaaaccggta gcctgaacct
     7261 gtacgtaatg gatttggctt ctggtcagat ccgccaggtg actgatggtc gcagtaacaa
     7321 taccgaaccg acctggttcc cggacagcca gaacctggca tttacttctg accaggccgg
     7381 tcgtccacag gtttataaag tgaatatcaa cggcggtgcg ccacaacgta ttacctggga
     7441 aggttcgcag aaccaggatg cggatgtcag cagcgacggt aaatttatgg taatggtcag
     7501 ctccaatggt gggcagcagc acattgccaa acaagatctg gcaacgggag gcgtacaagt
     7561 tctgtcgtcc acgttcctgg atgaaacgcc aagtctggca cctaacggca ctatggtaat
     7621 ctacagctct tctcagggga tgggatccgt gctgaatttg gtttctacag atgggcgttt
     7681 caaagcgcgt cttccggcaa ctgatggaca ggtcaaattc cctgcctggt cgccgtatct
     7741 gtgataataa ttaattgaat agtaaaggaa tcattgaaat gcaactgaac aaagtgctga
     7801 aagggctgat gattgctctg cctgttatgg caattgcggc atgttcttcc aacaagaacg
     7861 ccagcaatga cggcagcgaa ggcatgctgg gtgccggcac tggtatggat gcgaacggcg
     7921 gcaacggcaa catgtcttcc gaagagcagg ctcgtctgca aatgcaacag ctgcagcaga
     7981 acaacatcgt ttacttcgat ctggacaagt acgatatccg ttctgacttc gctcaaatgc
     8041 tggatgcaca tgcaaacttc ctgcgtagca acccgtctta caaagtcacc gtagaaggtc
     8101 acgcggacga acgtggtact ccggaataca acatctccct gggtgaacgt cgtgcgaacg
     8161 ccgttaagat gtacctgcag ggtaaaggcg tttctgcaga ccagatctcc atcgtttctt
     8221 acggtaaaga aaaacctgca gtactgggtc atgacgaagc ggcatactcc aaaaaccgtc
     8281 gtgcggtact ggtttactaa gagaattgca tgagcagtaa cttcagacat caactattga
     8341 gtctgtcgtt actggttggt atagcggccc cctgggccgc ttttgctcag gcaccaatca
     8401 gtagtgtcgg ctcaggctcg gtcgaagacc gcgtcattca acttgagcgt atttctaatg
     8461 ctcacagcca gcttttaacc caactccagc aacaactctc tgataatcaa tccgatattg
     8521 attccctgcg tggtcagatt caggaaaatc agtatcaact gaatcaggtc gtggagcggc
     8581 aaaagcagat cctgttgcag atcgacagcc tcagcagcgg tggtgcagcg gcgcaatcaa
     8641 ccagcggtga tcaaagcggt gcggcggcat caacgacgcc gacagctgat gctggtactg
     8701 cgaatgctgg cgcgccggtg aaaagcggtg atgcaaacac ggattacaat gcagctattg
     8761 cgctggtgca ggataaatcc cgccaggatg acgcaatggt ggcatttcag aatttcatca
     8821 aaaattaccc tgattcaact tacctgccaa acgccaatta ttggctgggt cagttaaact
     8881 acaacaaggg taaaaaagat gatgcggcgt actattttgc ttcggtagtg aaaaactatc
     8941 cgaagtcacc aaaggctgca gatgcgatgt ttaaagtcgg cgtcatcatg caggacaaag
     9001 gtgacaccgc aaaagcgaaa gccgtatacc agcaggttat cagtaaatac cctggtactg
     9061 atggcgctaa acaggcacaa aaacgtctga acgcgatgta atgcataaca cacgaccaga
     9121 agtcgcatta tttctggtcg tgtcgtgcga atcataagca gttgagtgat ctacatcgaa
     9181 atttttgttg cgctcaagtc tgaaatcagt aatatatgcc gccgttgcca cgggatatca
     9241 aacaaaccga aagcaacgaa aaagtgggtc gttagctcag ttggtagagc agttgacttt
     9301 taatcaattg gtcgcaggtt cgaatcctgc acgacccacc actaacctag ttagttgtag
     9361 tatccagcgt aatatcgggt gattagctca gctgggagag cacctccctt acaaggaggg
     9421 ggtcggcggt tcgatcccgt catcacccac caccgggtcg ttagctcagt tggtagagca
     9481 gttgactttt aatcaattgg tcgcaggttc gaatcctgca cgacccacca ctaattacgg
     9541 tgggttcggt ggaagtagtt tgtagtatcc agcgcagtat cgggtgatta gctcagctgg
     9601 gagagcacct cccttacaag gagggggtcg gcggttcgat cccgtcatca cccaccactc
     9661 gggtcgttag ctcagttggt agagcagttg acttttaatc aattggtcgc aggttcgaat
     9721 cctgcacgac ccaccaattt taacatcaaa ctcagatgtt aagcgtgaag gataacgttg
     9781 cgtcagcaac ggcccgtagg gcaagcgaag cgagtcatcc tgcacgaccc accaatttta
     9841 aagattggtt ccgagtagaa atctttcagg taacacccgt atgggtcgtt agctcagttg
     9901 gtagagcagt tgacttttaa tcaattggtc gcaggttcga atcctgcacg acccaccaat
     9961 gtaaaaaagc gccctaaagg cgcttttttg ctattcaggc atctctcaat ttcaattctt
    10021 gaccctgatg acatactccg cgcttgctgc gtaaacaact ttacgtcgga ggaataagac
    10081 gcaaagtgtg gcattcggca tctcatacgt aattcgagtg acttttcccc accattcgac
    10141 tatcttgttt agcatataaa acaaattaca ccgataacag cgaatatcac gctaatgtcg
    10201 gttttaacgt taagcctgta aaacgagatg gtaagatgag cgtgatgttt gatccagaca
    10261 cggcgattta tcctttcccc ccgaagccga cgccgttaag cattgatgaa aaagcgtatt
    10321 accgcgagaa gataaaacgt ctgctaaaag aacgtaatgc ggtgatggtt gcccactact
    10381 ataccgatcc cgaaattcaa caactggcag aagaaaccgg tggctgtatt tctgattctc
    10441 tggaaatggc gcgcttcggt gcaaagcatc ccgcttctac gttgttagtc gctggggtga
    10501 gatttatggg agaaaccgcc aaaattctca gtccggaaaa aacaattctg atgccgacac
    10561 ttcaggctga gtgttcactg gatctcggct gccctgttga agaatttaac gcattttgcg
    10621 atgcccatcc cgatcgtact gtcgtcgtct atgccaacac ttctgctgcg gtaaaagcgc
    10681 gggcagattg ggtggtaact tcaagcattg ccgtcgaact tattgatcat ctggatagtt
    10741 tgggtgaaaa aatcatctgg gcacccgaca aacatctggg gcgttacgtg caaaaaaaga
    10801 cgggcgcaga cattttatgc tggcagggtg cctgcattgt gcatgatgag tttaagactc
    10861 aggcgttaac ccgcttgcaa gaagaatacc cggatgctgc catactggtg catccagaat
    10921 caccacaagc tattgtcgat atggcggatg cggtcggttc caccagtcaa ctgatcgctg
    10981 ctgcgaaaac attgccacat cagaggctta ttgtggcaac cgatcggggt attttctaca
    11041 aaatgcagca ggcggtgcca gataaagagt tactggaagc accaaccgcg ggtgagggcg
    11101 caacctgccg cagctgcgcg cattgtccgt ggatggccat gaatggcctc caggccatcg
    11161 ctgaggcatt agaacaggaa ggaagcaatt acgaggttca tgttgatgaa aggctgcgag
    11221 agagggcgct ggtgccgctc aatcgtatgc tggattttgc ggctacacta cgtggataac
    11281 gaataataag gcgtaacgtt acgctttggg ggaaagatgg atttttttag tgtgcagaat
    11341 atcctggtac atataccaat aggagcaggc ggttatgatc tctcatggat cgaagcggta
    11401 ggcacaatcg ccgggttgct gtgtattggc cttgccagtc tggagaagat cagcaactac
    11461 ttctttggcc tgatcaacgt caccttgttt ggcattattt tctttcagat tcagctgtat
    11521 gccagcctgc tattacaggt gtttttcttt gccgcgaata tttacggctg gtatgcgtgg
    11581 tcgcgacaaa ccagtcagaa cgaggcggag ttaaaaattc gctggttgcc attgccgaag
    11641 gcactcagct ggttggcggt ttgcgttgtt tcgattggtc tgatgacggt atttatcaat
    11701 ccggtgtttg catttttgac ccgcgtggca gtcatcatca tgcaagcatt aggattacag
    11761 gttgcgatgc ctgaactaca accggacgct ttcccgttct gggattcttg catgatggtg
    11821 ttatctatcg tggcaatgat tctgatgact cgtaagtatg tggaaaactg gctgttgtgg
    11881 gtgattatta acgtgattag cgtcgttatt tttgcacttc agggcgttta cgccatgtct
    11941 ctggagtaca tcatcctgac ctttattgcg ctcaacggca gccggatgtg gatcaatagc
    12001 gcacgcgaaa gaggctcacg cgcgctgtcc cattaatggt gatgatgtga atggccagac
    12061 accccttcat tcaggtggca gtctggccca tgacaaggtt gatattccat ctgaatggtg
    12121 gcgtgctcaa tctgatagtg atccatcagg tagtgttgga tctgatccaa caaggcatcg
    12181 tgatcgtgtg gcgggatcac ctgcacatgc aacgtcatca ccggcttctc gcctaccatc
    12241 catacatgca catggtgcac attgcggact tccgggattt cccggcacat acgacgcttc
    12301 agttcagcga tatccagcga taccggtgca ccctcaagta attcattcac gctatctttc
    12361 aacagtcgcc acgcgctacg cagaaccaga agcgacacca gtatcgagag aatggggtca
    12421 gcaggtgtcc agccggtcca gataataatc aacgcggcaa taatagcccc aaccgaaccc
    12481 agcaggtccc cgagcacatg cagtgccgct gctcgtacgt tgaggttttt ctcttcgctg
    12541 ccgtgatgaa gtaaccagaa agaaagtata tttgccagca acccagccac ggcaattgcc
    12601 atcatcatgc cgccctcgac cggacgcggc gtgcggaagc gttctatcgc ctcccagaca
    12661 atcaaaatgg taattaccac gagggcgatg gcattcacaa acgccgcgag cgtggtcaat
    12721 cttagccaac cgaaagtgtg gcgaatggtg ggaggacgac gtgaaaattg cacggcgagt
    12781 agggcgaaaa gcagggcggc agtatcggtc aacatatgac ccgcatcggc cagcaaagcc
    12841 agagaaccgg aaagaaaacc accaatgact tctaccagca taaacccggc agtcacgccg
    12901 aaagcataca acaagcgacg agcattatta tcttctggca ggtgtgagga cgtgtgtgag
    12961 tgtgagtgcg ccatgacgag tcatccctta attattctca cttaacttta tgacatcata
    13021 ctgcttttag aagggaaaaa ttaaaaggga gagactccgc tctcccatta ttggctattt
    13081 tgcagggtta ctgcgtggta ccgtcggttt tggtatcgac atcattattg atgccatcac
    13141 cggtttgtac ctttttattg atatccgggc agcgaccatc tttgcacatg gtgttcttgt
    13201 gctcttcatc tttggtcatg ccgtcattgt tcattgaaga accatccgga tgcagcatag
    13261 tgccgccaga accggtattt accccgttat tgtcgacgtt atttggcgcg acattttcac
    13321 gggcgtcagg ggctacctgg cccgcatcag ctgcggcgtt tgcctggccg ttattggttt
    13381 gcgctccgct atcggcagcc agtgcggcac cgctggcaag gcttagagtg gcagtcagaa
    13441 atagtgtggc cagttttgtc attttcatag gatgctcctg ttatggtcgt tatgtcggat
    13501 aacctcttcc aacagtgcat ttgcaggtga atataaggca ttggtttaag atttcagcca
    13561 ggcaaagaaa tccagcagag aatcttgaaa taattaacaa acaaaggagt tacagttaga
    13621 aattgtagga gagatctcgt ttttcgcgac aatctggcgt ttttcttgct aattccagga
    13681 ttaatccgtt catagtgtaa aaccccgttt acacattctg acggaagata tagattggaa
    13741 gtattgcatt cactaagata agtatggcaa cactggaaca gacatgaatt atcagaacga
    13801 cgatttacgc atcaaagaaa tcaaagagtt acttcctcct gtcgcattgc tggaaaaatt
    13861 ccccgctact gaaaatgccg cgaatacggt tgcccatgcc cgaaaagcga tccataagat
    13921 cctgaaaggt aatgatgatc gcctgttggt ggtgattggc ccgtgctcaa ttcatgatcc
    13981 tgtcgcggct aaagagtatg ccactcgctt gctggcgctg cgtgaagagc tgaaagatga
    14041 gctggaaatc gtaatgcgcg tctattttga aaagccgcgt actacggtgg gctggaaagg
    14101 gctgattaac gatccgcata tggataacag cttccagatc aacgacggtc tgcgtatagc
    14161 ccgtaaattg ctgcttgata ttaacgacag cggtctgcca gcggcgggtg aattcctcga
    14221 tatgatcact cctcagtatc tcgctgacct gatgagctgg ggcgcaattg gtgcacgtac
    14281 cacggaatcg caggtgcacc gcgaactggc gtctggtctt tcttgtccgg taggttttaa
    14341 aaatggcaca gacggtacga ttaaagtggc tatcgatgcc attaatgccg ccggtgcgcc
    14401 gcactgcttc ctgtccgtaa ctaaatgggg gcattcggcg attgtgaata ccagcggtaa
    14461 cggcgattgc catatcattc tgcgcggcgg taaagagcct aactacagcg cgaagcacgt
    14521 tgctgaagtg aaagaagggc tgaacaaagc aggtctgcca gctcaggtga tgatcgattt
    14581 cagccatgct aattcgtcca aacaattcaa aaagcagatg gatgtttgtg ctgacgtttg
    14641 ccagcagatt gccggtggcg aaaaggccat tatcggtgtg atggtggaaa gtcatctggt
    14701 ggaaggcaat cagagcctgg agagcgggga gccgctggcc tatggtaaga gcatcaccga
    14761 tgcctgcatt ggctgggaag ataccgatgc tctgttacgt caactggcga acgcagtgaa
    14821 agcgcgtcgc gggtaaagtt taattgtcgg atgcgccgtc agagtggcgt atccgatgaa
    14881 tcaccgtagc cctgacaaga cgcgcagcgt cgcatcaggc aatgtgctcc attgttagcg
    14941 acgacaaaca aaaaagccga ctcacttgca gtcggctttc tcatttttaa acgaatgacg
    15001 tttacttcgc tttaccctgg ttcgcaacgg ctgctgcttt cgccgcgatc tcgtcagcat
    15061 tacccagata atagcgtttc agcggtttga agttctcgtc aaactcataa accagcggca
    15121 cgccggtcgg gatattcagc tcaagaatct cttcttcgct catgttatcg agatatttca
    15181 ccagcgcacg taacgagtta ccgtgtgcag cgatgattac gcgctcaccg ctcttcatac
    15241 gcggcagaat ggtttcattc cagtaaggga tcacgcggtc aatggtcagc gccaggcttt
    15301 ccgtcagcgg cagttctttc tcgctcagtt tcgcgtaacg cggatcgtga cccggataac
    15361 gctcatcatc tttagtcagt tccggcggag tcactgcaaa accacgacgc cactgtttca
    15421 cctgctcgtc gccatatttt tcagcagttt ccgctttgtt cagaccctgc aacgcaccgt
    15481 agtgacgttc gttcagtttc caggatttct caacgggcag ccatgcctga tccagttcgt
    15541 ccagcacatt ccacagggta tggatagcgc gtttcagcac agaggtgtaa gcaaagtcaa
    15601 agctgtaacc ttcctctttc agcagcttac ctgctgcttt tgcttcgctt acgcctttct
    15661 cagacagatc cacgtcgtac caaccggtga aacggttttc tttgttccac tgactttcgc
    15721 catgacgaac cagaaccagc ttagttacag ccatatactt actcctcaaa tcatctttta
    15781 atgataataa ttctcattat attgccgtga cgaagcaaca gcaatgctta cgcataacca
    15841 tagcgaaaat agtggcgcag tgtaaggttg ttgtgaatat tgagttgcaa atatgtcggt
    15901 gtttgctggt gatttgaaca atatgagata aagccctcat gacgagggct taacattact
    15961 cagcaataaa ctgatattcc gtcaggctgg aatactcttc accaggatgc aggaaacagt
    16021 ccggttgcgg ccattcaggg tggttcgggc tgtccggcag aaactcgctt tccaacgcca
    16081 gcccttgcca gtcggcgtaa ggttcggttc cccgcgacgg tgtgccgccg aggtagttgc
    16141 cggagtagaa ttgcagagcc ggagcggtgg tgtagacctt cagctgcaat ttttcatctg
    16201 ctgaccagac atgcgccgcc actttcttgc catcgccttt ggcctgtaac aagaatgcgt
    16261 gatcgtaacc tttcactttg cgctgatcgt cgtcggcaag aaactcactg gcgatgattt
    16321 tggcgctgcg gaaatcaaaa gacgttccgg cgacagattt caggccgtcg tgcggaatac
    16381 cgccttcatc aaccggcaga tattcgtccg ccagaatctg caacttgtga ttgcgcacgt
    16441 cagactgctt gccgtcaaga ttgaaataga cgtgattagt catattcacc gggcaaggtt
    16501 tatcaactgt ggcgcgataa gtaatggaga tacggttatc gtcggtcaga cgatattgca
    16561 ccgtcgcgcc gagattaccc gggaagccct gatcgccatc atctgaactc agggcaaaca
    16621 gcacctgacg atcgttctgg ttcacaatct gccagcgacg tttgtcgaac ccttccgggc
    16681 cgccgtgcag ctggttaacg ccctgacttg gcgaaagcgt cacggtttca ccgtcaaagg
    16741 tataacgact attggcgatg cggttggcat aacgaccaat agaggcaccc agaaacgcgg
    16801 cctgatcctg atagcattcc gggctggcac agccgagcag cgcctcgcgg acgctgccat
    16861 cggaaagcgg aatacgggct gaaagtaaag tcgcacccca gtccatcagc gtgactacca
    16921 tccctgcgtt gttacgcaaa gttaacagtc ggtacggctg accatcgggt gccagtgcgg
    16981 gagtttcgtt cagcactgtc ctgctccttg tgatggttta caaacgtaaa aagtctcttt
    17041 aatacctgtt tttgcttcat attgttctgc gacagcttgc tgtacggcag gcaccagctc
    17101 ttccgggatc agcgcgacga tacagccgcc aaatccgccg ccggtcatgc gtacgccacc
    17161 tttgtcgcca atcacagctt tgacgatttc taccagagtg tcaatttgcg gcacggtgat
    17221 ttcgaaatca tcgcgcatag aggcatgaga ctccgccatc aactcgccca tacgtttcag
    17281 gtcgccttgc tccagcgcgc tggcagcttc aacggtgcgg gcgttttcag tcaggatatg
    17341 acgcacgcgt ttcgccacga ttgggtccag ctcatgtgca acagcgttga actcttcaat
    17401 ggtgacatcg cgcagagctg gctgctggaa gaaacgcgca ccggtttcgc actgttcacg
    17461 acgggtgttg tattcgctgc caaccagggt acgtttgaag ttactgttga tgatgacgac
    17521 agccacacct ttcggcatgg aaactgcttt ggtccccagt gagcgacagt caatcagcaa
    17581 ggcatgatct ttcttgccga gtgcggaaat tagctgatcc atgatcccgc agttacagcc
    17641 aacaaactgg ttttctgctt cctgaccgtt aagcgcgatt tgtgcgccgt ccagcggcag
    17701 atgataaagc tgctgcaata cggttccgac cgcgacttcc agtgaagcgg aagaacttaa
    17761 cccggcaccc tgcggcacat tgccgctgat caccatgtcc acaccgccga agctgttgtt
    17821 acgcagttgc agatgtttca ccacgccacg aacgtagttc gcccattgat agttttcatg
    17881 cgcgacaatg ggcgcatcaa gggaaaactc atcaagctga ttttcataat cggctgccat
    17941 tacgcgaact ttacggtcat cgcgtggcgc acagctgatc acggtttgat aatcaatcgc
    18001 gcagggcaga acgaaaccgt cgttgtagtc ggtgtgttca ccaatcaaat tcacgcggcc
    18061 aggcgcctga atggtatgag tggcagggta gccaaatgcg ttggcaaaca gagattgtgt
    18121 tttttctttc agactcattt cttacactcc ggattcgcga aaatggatat cgctgactgc
    18181 gcgcaaacgc tctgctgcct gttctgcggt caggtctcgc tgggtttctg ccagcatttc
    18241 ataaccaacc ataaatttac gtacggtggc ggagcgcaac agaggcggat aaaagtgcgc
    18301 gtgcagctgc cagtgttgat tctcttcgcc attaaacggt gcgccgtgcc agcccataga
    18361 gtaggggaag gagcactgga agaggttgtc ataacgactg gtcagctttt tcaacgccag
    18421 tgccaaatcg ctgcgctggg cgtcggtcaa atcggtgatc cgcaaaacgt gggctttggg
    18481 cagaagtagc gtttcgaacg gccaggcagc ccagtaaggc acaactgcta accagtgttc
    18541 ggtttcgaca acggtacggc taccgtctgc cagctcgcgc tgaacataat ccaccagcat
    18601 tggcgatttc tgctcggcaa aatattcttt ttgcaggcgg tcttcgcgct cagcttcgtt
    18661 aggcaggaag ctatttgccc aaatctgtcc gtgcggatgc gggttagagc agcccatcgc
    18721 cgcacctttg ttttcaaaga cctgcaccca cgggtatgtt ttccccagtt ctgcggtttg
    18781 ctcctgccag gttttgacga tttccgtcaa tgccgcaacg ctcagttctg gcagcgtttt
    18841 actgtgatcc ggtgaaaagc agatcacccg gctggtaccg cgcgcgctct ggcaacgcat
    18901 caaaggatcg ttgctttctg gcgcatctgg cgtgtcagac atcaaggccg caaagtcatt
    18961 agtgaaaacg taagtcccgg tgtaatcggg gtttttatcg cctgtcaccc gcacattacc
    19021 tgcgcagagg aagcaatctg gatcgtgcgc aggtaacacc tgtttggctg gcgtttcctg
    19081 cgccccctgc caggggcgct tagcgcggtg cggtgaaacc agaatccatt gcccggtgag
    19141 ctggttgtag cggcgatgtg gatgatcaac gggattaaat tgcgtcatgg tcgttcctta
    19201 atcgggatat ccctgtggat ggcgtgactg ccagtgccag gtgtcctgcg ccatttcatc
    19261 gagtgtgcgc gttacgcgcc agttcagttc acggtcggct ttgctggcgt ccgcccagta
    19321 ggccggaagg tcgccctcgc gacgcggtgc aaaatgatag ttaaccggtt tgccgcaggc
    19381 tctgctgaag gcattaacca cgtccagcac gctgctgcct acgccagcac cgaggttgta
    19441 gatgtgtacg cctggcttgt tcgccagttt ttccatcgcc acgacgtgac cgtccgccag
    19501 atccattacg tggatgtaat cgcgtacgcc agtaccgtct tcggtcggat aatcgttacc
    19561 aaaaatcgcc agcgagtcgc gacggcctac agcaacctgg gcgatgtatg gcataaggtt
    19621 attcggaatg ccttgcggat cttcgcccat atcgcccgac ggatgtgcgc caaccgggtt
    19681 gaagtagcgc agcagggcaa tgctccagtc cggctgggct ttttgcagat cggtgaggat
    19741 ctgttccacc atcagcttgc ttttgccgta agggctttgc ggtgtgccgg tcgggaagct
    19801 ttcaacgtat ggaattttgg gctgatcgcc ataaacggtg gcggaggagc taaaaataaa
    19861 gtttttgacg ttagcggcgc gcatggcgct aatcaggcgc agagtaccgt tgacattgtt
    19921 gtcgtaatat tccagcggtt tttgtaccga ttcgccaacg gctttcagcc cggcgaagtg
    19981 gatcacggtg tcgatagcgt gatcgtgcag gatctcggtc atcaacgctt cgttacggat
    20041 atcgccttca acaaacgtcg gatgtttgcc gcctaaacgc tcgataacag gcagtacgct
    20101 gcgcttactg ttacagaggt tatcaagaat gatgacatca tgaccgtttt gcagtaattg
    20161 cacacaggta tgacttccaa tgtaaccgct accaccagtg accagaactc tcataattcg
    20221 ctccattagg cttatggtat gaaataacca tagcataaca aagatgcgaa aagtgtgaca
    20281 tggaataaat tagtggaatc gtttacacaa gaatttagcc gttttttatg cgcgattaag
    20341 tgattataaa acagagggat tgtgaatgat tgcgcttttt atctgaaaaa aagacgcagt
    20401 ttcatgcctg gatgcgtctg accgtgtgcc ggataaagcg tttacgccgc atccggcaca
    20461 gtcagcatta ccgacttagt ttatttttgt cagcgcataa tgatagaggc caccgtccgg
    20521 cacgaactcc agacgatggg taatacaagc cggcgcatct tcagcatggt gcgaaacaaa
    20581 caacaattgc gtttcacctt cgctaatcag cacatcaaca aaacggcgaa taagctggcg
    20641 attgagcgga tcaagcccct gcaatggttc gtcgagaata agcaacgtcg gatgtttcac
    20701 cagtgcgcgg acgatcagcg ccagacgctg ctgtccccag gaaagactat ggaacggagc
    20761 gtcagccgtg cgtttatcaa tgccgagaat atccagccac tgctgcacca gtttttgctg
    20821 gcgatccgaa acggcctgat aaatgccaat cgaatcaaaa tagccagaaa gaatcacatt
    20881 acgcacagta gtactgaccc ggtaatccag atgcaaacta ctgctgacgt aaccgatatg
    20941 ctttttgata tcccagatgg tttcgccgct gccacgacgt cgtccgaaaa gcgtcaaatc
    21001 gttgctgtaa ccttgcggat gatcgccagt aatcaggctt aataacgtcg attttcccgc
    21061 gccatttggc ccgacaattt gccagtgttc acctggattc acctgccagc taaggttatt
    21121 aagaatgggg cgatcgttat aagaaaccac gccattattc agcacaatgc gcggttcgtt
    21181 ggcgggtaag gcgtgacgtg ctgaaggttc atccggctcc ggcagttgca caccttcaag
    21241 ctgttcacta tgcgccagtt gcgcgacgag tgcttgttgg agcagttcct ctttagcgcc
    21301 agtttccgct aacgtgcaat ccgccagcac gccagcaaac tggacaaact ccgggatctc
    21361 atcgaagcga ttgagcacca gtaccagagt aataccggac tgatgtaacg aggcgagtcg
    21421 ctcagccagc tgctgacgtg aggcaacatc caggccatcg aacggctcat caagaatcaa
    21481 caagtcaggc tccgacatca gcgcctgaca cagcagggtt tttcgcgtct cgccagtgga
    21541 aaggtattta aagcgtcggt cgaggagggg ggtaataccg aactgctgtg ccagttgcat
    21601 gcagcgcggt gcatccttta cttcatcctg aataatctca gccgtagtgc gtccggtgtc
    21661 atcttcgcca gggccgagca tatcggtgtt attccgctgc cattcgtcgc tgacgagctt
    21721 ttgcaattgc tcgaaggaga gacgagtgat gtgggaaaac tggctttgcc gttcaccttt
    21781 caaaagcgga agttcccccg ccagcgcgcg ggccagggcc gatttcccgc ttccattcga
    21841 accgacaaac gcccaactat cacccgcgtt taacgttagc tgaggcaatt gcagcgtttt
    21901 tgtgtcgcta agacgaaacg tgccttgcaa aatttgcaac gatgacattt tatatcccat
    21961 tttgtgcagc gattactgac agggatacgt gtttcataac aaattgtcaa cacgcttagc
    22021 acagtgtggc gataatcacg ctgtcggcat taaagtaggc cgtgacattc tgtccttgct
    22081 gaagagaagt cgcttcattt accggcactg tggcgcacag tgtttgcccg tccggtagcg
    22141 ccattaatac ttcgcactgc tctgcgccgc gctcaatatg actaataata cccggtaatt
    22201 ggttgtcagc gttttgcgcg accgcttcgt cctgagtaat gcctacccac ggcgctttta
    22261 gcaatatcaa cacttctttg ccttcatcca gccccagacg cgcgccgctt tgtgcggtaa
    22321 ttgcgacttt caggcgtgtt tttccatcag ccagtaagac atcaacatgc tgttgaacgt
    22381 catcatgatc gcgggcggtg atggtaccga accactggtt acgggcgctg gtttggagtg
    22441 aaaaacgtga gatcgcggcc agcaggctgt tcagcggcag ggcgtcatcg tcacttaaca
    22501 catcaaaggc tttttgctgg atttgcgcca gtaagtcata gagctgaatc agtcgctgac
    22561 catagcgggt cagtactgcg ccgccgccac ctttaccgcc tgttgcgcgc tcgaccagaa
    22621 tatgctcact taactgattc atctcgttaa tggcatccca ggcgctttta tagctgatac
    22681 cggcatcttt cgctccctgg ctaatggaac cggaaagcgc aatgtgtttt agtagcgaaa
    22741 tgcggcgcgg gtcggcgaat aatttttgtt ggagcttaag ggtgagaagg atttcggcct
    22801 gcataacaat gtcctggcaa aagtcttatt gtgacggaaa acgaacgcca cgcaaagctg
    22861 accgcacaaa aggggagtgc ttttctgtgc ttagcggtta gaatagtctc atgactatat
    22921 ctggagttga ccatgttaga gttattaaaa agtctggtat tcgccgtaat catggtacct
    22981 gtcgtgatgg ccatcatcct gggtctgatt tacggtcttg gtgaagtatt caacatcttt
    23041 tctggtgttg gtaaaaaaga ccagcccgga caaaatcatt gattccctga atgcccgctt
    23101 agtcgggcat tttctttttc tcaacttcct gcttttcctg ccgatatttt ttcttatcta
    23161 cctcacaaag gttagcaata actgctggga aaattccgag ttagtcgtta tattgtcgcc
    23221 tacataacgt tacattaagg ggttaccaat ggctcgtaaa tggttgaact tgtttgctgg
    23281 ggcggcactc tctttcgctg ttgctggcaa tgcactggca gatgaaggga aaatcacggt
    23341 gttcgccgcc gcatcactga ctaacgcaat gcaggacatt gctacgcagt ataaaaaaga
    23401 gaaaggcgtg gatgtggttt cttctttcgc ttcgtcatct actctcgccc gtcagattga
    23461 agcgggtgcc cctgcggatc tgtttatttc tgccgatcag aaatggatgg attatgcggt
    23521 tgataaaaaa gcgatcgata cagctacgcg tcagacactg ctcggcaata gcctggtcgt
    23581 tgtagcaccg aaagccagcg agcagaaaga ttttaccatc gacagcaaaa ccaactggac
    23641 ttcactgctg aatggcggtc gcctggcggt tggcgatccg gaacatgttc ccgctggcat
    23701 ttatgcaaaa gaagcactgc aaaaactggg cgcatgggat acgctctccc cgaaactggc
    23761 tccggcggaa gatgttcgtg gcgcgctggc gttggtcgaa cgcaatgaag cgccgttggg
    23821 cattgtttac ggttctgacg cagttgctag caaaggggta aaagtggttg ccaccttccc
    23881 ggaagattca cataaaaaag tggaatatcc ggttgctgtt gtggaagggc ataacaatgc
    23941 gacagtgaaa gcattttatg attatctgaa gggaccgcag gcagcggaaa tctttaaacg
    24001 ttacggattt acaaccaagt aatgatactg accgatccag aatggcaggc agttttatta
    24061 agcctgaaag tttcttccct ggctgtgctg tttagcctgc cgtttgggat cttttttgcc
    24121 tggttactgg tgcgttgcac gtttccgggc aaagctctgc tcgacagcgt actgcatcta
    24181 ccgctggtgt taccgcccgt ggtcgtcggt tacttattat tagtttcgat ggggcggcgc
    24241 ggatttatcg gtgaacgtct gtatgactgg tttggtatta ccttcgcctt tagctggcgc
    24301 ggcgcggttc tcgctgccgc cgtcatgtcg tttccgctga tggtgcgggc aattcgtctg
    24361 gcgctggaag gggttgatgt caaactggaa caggccgcaa gaacactggg ggccgggcgc
    24421 tggcgcgttt tctttactat cacgttaccg ctgaccttac cgggaattat tgttggtacg
    24481 gtactggctt ttgctcgttc tctcggtgaa tttggcgcaa ccatcacctt tgtgtcgaac
    24541 attcctggtg aaacgcgaac cattccttct gccatgtata ccctgattca gacccccggc
    24601 ggggaaagtg gcgcagcgag actgtgcatt atttctattg cgctggcgat gatctccctg
    24661 ttgatttcag aatggctggc cagaatcagc cgtgaacggg cggggcgcta atcatgctgg
    24721 aactgaattt ttcccagacg ttgggcaacc attgcctgac tattaatgaa acgctgcccg
    24781 ccaatggcat cactgctatc tttggcgtct ccggtgccgg aaaaacttcg ctgattaacg
    24841 ccatcagtgg actgacgcgc ccgcaaaaag ggaggattgt cctcaatggt cgggtactaa
    24901 atgatgccga aaaaggtatc tgcctgacgc cggaaaagcg tcgcgttggc tatgtttttc
    24961 aggatgcgcg gctgttcccg cattacaaag tgcgtggcaa tctgcgctac ggcatggcga
    25021 aaagcatggt cgatcagttc gataagctgg tggcgctttt aggcattgaa ccgttgcttg
    25081 accgtttacc aggcagcctg tccggaggcg aaaaacagcg cgtagcgatt gggcgggctt
    25141 tgctgacggc accagaattg ctgttgctgg atgaaccgct ggcgtcactg gatattccgc
    25201 gtaaacgcga actgttgcct tatctgcaac gtctgacgcg ggaaatcaac atcccgatgt
    25261 tgtatgtcag ccattcgctg gatgagatcc tccatctggc agacagagtg atggtactgg
    25321 aaaacggtca ggtgaaagcc tttggcgcgc tggaagaagt ctggggtagt agcgtgatga
    25381 atccgtggct gccgaaagag caacaaagta gcattctgaa agtgacggta cttgagcatc
    25441 atccgcatta cgcgatgacc gcgctggcgc tgggcgatca gcatttgtgg gtcaataagc
    25501 tggacgaacc gctgcaagct gcgctacgta tccgcattca ggcttccgat gtttctctgg
    25561 ttttacaacc gccgcagcaa accagcattc gtaacgtatt gcgggcaaaa gttgttaata
    25621 gttatgacga caacggccag gtggaagtgg aactggaagt cggcggtaaa acgctgtggg
    25681 cgcgtatcag cccgtgggcc agggatgaac tggcgatcaa acctggcctg tggctgtacg
    25741 cgcaaattaa aagtgtgtcg ataaccgcct gattaaatca ggtggctata aatgaactgg
    25801 gcaatgctgt cggtggtgtt atcaccaatc acaatgttgg cgcgcgcttt taccgcgtca
    25861 tcggcgttgc ccatcgccac gcctgtacca gcggcctcca gcatactgat atcattaaag
    25921 ttatcgccga acgccacgac gttttccatc gaccagcctt gcgcctcaac ccatttcgtc
    25981 aaacgtttac ctttgctgtt gccgccgcgt gcaatatcaa cctgatcgtg ccaggaccat
    26041 tcacactcca gtcccagttc atgttcgaca tgcttaccaa aatgctgcaa ttgcggcagg
    26101 tcatcgtgcg tgagggcgaa cttccatacg gcgttaactt gttgcgccgt ttcagccaga
    26161 gaagcgactt gtgtgaaagt cggacgctgt tccggcggca gggtttgcgc ccagttagac
    26221 gtgcgaatga catgcccggt cgggtgctca taaaccatct cgtcatcgac atacatcaga
    26281 ccgtgaatgt ggtgttcatt cagcatctca atgagttgta gggctttatt aacgggcatt
    26341 gggtccgctt ccagcacggt ttttgcatga taatcataca aataggtgcc attacagcaa
    26401 atagcaggtg tatccagcgc cagcgcctga taaaaaggat gaatagcgac gtgatggcga
    26461 cctgtgacga tgattaattg ataacctgct tcgcgagcgc gggccagggc ttctatcgat
    26521 gaaggtagca gggtcttttt cggggtcaat aaggtgccgt ctaagtcgag agcaatcacg
    26581 cgtgtggtca tggcgtattc cagattaagg ttaagaattt tcttctgcgc gaatggtaca
    26641 ccgataccac tatcggacaa aattctgcgt tttaattcag cattcaccgc caaaagcgac
    26701 taattttagc tgttacagtc agttgctaaa tgcaaaggag cattcatgaa gcaaacagtt
    26761 tatatcgcca gccctgagag ccagcaaatt cacgtctgga acctgaatca tgaaggcgca
    26821 ctgacgctga cacaggttgt cgatgtgccg gggcaggtgc agccgatggt ggtcagcccg
    26881 gacaaacgtt atctctatgt tggtgttcgc cctgagtttc gcgtcctggc gtatcgtatc
    26941 gccccggacg atggcgcact gacctttgcc gcagagtctg cgctgccggg tagtccgacg
    27001 catatttcca ccgatcacca ggggcagttt gtctttgtag gttcttacaa tgcgggtaac
    27061 gtgagcgtaa cgcgtctgga agatggcctg ccagttggcg tcgtcgatgt ggtcgagggg
    27121 ctggacggtt gccattccgc caatatctca ccggacaacc gtacgctgtg ggttccggca
    27181 ttaaagcagg atcgcatttg cctgtttacg gtcagcgatg atggtcatct ggtggcgcag
    27241 gaccctgcgg aagtgactac cgttgaaggg gccggcccgc gtcatatggt attccatcca
    27301 aacgaacaat atgcatattg cgtcaatgag ttaaacagct cagtggatgt ctgggaactg
    27361 aaagatccgc acggcaatat cgaatgtgtc cagacgctgg atatgatgcc ggaaaacttc
    27421 tccgacaccc gttgggcggc tgatattcat attaccccgg atggtcgtca cttatatgcc
    27481 tgtgaccgta ccgccagcct gattactgtc ttcagcgttt cggaagatgg cagcgtgttg
    27541 agtaaagagg gcttccagcc aactgaaacc cagccgcgcg gctttaatat cgatcaccgt
    27601 ggtaagtatc tgattgccgc cgggcaaaaa tcgcaccaca tctcggtata cgaaattgtt
    27661 ggtgagcagg ggctactgca tgaaaaaggc cgctacgcgg tcgggcaggg accaatgtgg
    27721 gtggtggtta acgcacacta accgctgatt tacccggcgc agtctctcct gcgccggtgt
    27781 attaacctat ctcctgtaac gcatgtctct ggcgttcgac gatattggtc cacaaattgt
    27841 ctttatcgtc agtccacaaa ttaatcagca aggcaaaaaa gcgttctgct gccggagaaa
    27901 gcacggcatc tttgcggcga ataatcccca atgtccgacg tataacgggt tccaccagtg
    27961 ggataccaat aatagaagaa tagggcgcat ggggcattgc cagaccagga agcgccgaaa
    28021 ttcccagtcc cgcctctacc agtcctaatg acgtcgaaag atgacgcact tcgtaaaacc
    28081 agtccagctt ccagggcttg tcggctagct gttgttctat caacaatcgg ttgccactgg
    28141 aggaacgtac gccaatcatt ttgtaaccca ctaactcctg ccattctaca agctgctttt
    28201 tagccaatgg atggtcacgc cgacaggcca gcacgaacgg ctcgttgacc agtggggtaa
    28261 aatcaatgga tgaatttgtg acgttgttca tgtttatgcc aaagtctgat tcgttacaca
    28321 gcaccgattc catgcaatta ttcgtgcctt gttccagaat ccgcacttta atattgggat
    28381 acagctcatt aaatttaccg attgccagcg gtaaaaaata aaatactgcg gtcggaatac
    28441 acgctaacgt caccatacca cgatgataag cattcatatc acgaatatta aaaagcgttt
    28501 catcgaattt ctttattaat tcccgcgcct ctgggagcaa cctttttcct gcttttgtta
    28561 acgtaacctt gcgagttgta cgctcaaata gttgaatatg taaatcttcc tccatctttt
    28621 ttattcttcg tgttaatgca ggttgcgtaa tattgagtaa tttagcggca ttgttaaatg
    28681 aactggactc tgccagtatg acaaatgcct tcatacttga taattcatgc ttcattatta
    28741 ctccgggaaa tggaagcgac gattttgggt ggctggccgt taaaaatttt aactgcattt
    28801 agccaactta aattaatgaa aaaatgttat taatcgttga gctaaagtca ttagagatgc
    28861 tttgccctta atgtaaccat atcgcaataa gttatgtttt taaattgagg gcattattat
    28921 gaaaaaaata ccctgcgtga tgatgcgagg tggaacctcg aggggcgcgt tcctgttagc
    28981 ggaacattta cccgaagatc aaacgcagcg cgataaaata ttgatggcaa ttatgggttc
    29041 cggtaacgat ctggaaattg acggtattgg cggcggtaat ccactgacca gtaaagtcgc
    29101 cattattagc cgttccagcg atccgcgtgc tgatgttgat tatctgtttg ctcaagttat
    29161 cgtccatgag aaacgtgtcg ataccacgcc taactgcggc aatatgctgt ctggcgttgg
    29221 ggcattcgcc attgaaaatg gtttgattgc agcgacttcg ccagttaccc gcgtacgtat
    29281 ccgcaacgtc aatacgggta cgttcatcga agctgatgtg caaacgccaa atggtgttgt
    29341 cgagtacgag ggtagcgcca gaattgacgg cgtaccgggt actgccgcac cggttgcgct
    29401 cactttcctg aatgccgctg gaacgaaaac cggaaaagtt ttcccgactg ataatcagat
    29461 tgattatttt gacgatgtcc cggtgacctg tatcgatatg gcgatgccag tcgtcattat
    29521 tccggctgaa tatctgggaa aaacaggcta tgaattaccg gcggaactgg atgccgacaa
    29581 agcattatta gcccgcattg aatctatccg tctacaagcg ggtaaagcaa tgggcttagg
    29641 cgatgtcagt aatatggtta tccctaaacc tgtgcttatt tctccagcgc agaaaggcgg
    29701 ggcaattaat gtgcgttatt ttatgccgca ttcttgccat cgcgcgctgg cgataaccgg
    29761 tgctattgct atttccagta gttgtgcatt ggaaggtacc gtcacccgac aaatcgtccc
    29821 ttctgtagga tacggcaata tcaatattga acaccccagt ggtgggctcg acgttcattt
    29881 aagtaatgaa ggtcaggatg ccacgacgtt acgcgcatct gttattcgga caaccagaaa
    29941 aatattttcc ggtgaagttt atcttccctg aaaagattcg ttgtcaggat aaggacaatc
    30001 aataaaggac ttctgtatga gtcatacaga aagaacagga ttttaaatga ataagaaatc
    30061 gttatggaag ctaattctga tattagcgat cccatgtatt attggcttta tgccagctcc
    30121 ggcaggatta agcgaactgg cgtgggtgct ttttggtatt tacctggcgg ccattgtggg
    30181 gctggttatc aagcctttcc cggaacctgt cgtactgtta attgccgttg ctgcctccat
    30241 ggtggtggtc ggtaacttat ccgacggtgc gtttaagacc accgccgtat taagcggtta
    30301 ctcttcaggt actacctggc tggtgttctc ggcgtttacc ttaagcgccg catttgtgac
    30361 caccggttta ggtaaacgta ttgcctatct gctgattggt aaaatcggta acaccacgct
    30421 gggtctgggt tacgttacgg tattcctcga tctggtattg gctccggcaa caccgtctaa
    30481 caccgcgcgt gcgggcggca ttgtgttacc gatcatcaac agcgtggcgg tggctttggg
    30541 gtccgaaccg gaaaaaagtc cgcgtcgtgt cggacattac ctgatgatgt ccatttacat
    30601 ggtcaccaaa accaccagct atatgttctt taccgcaatg gcggggaaca ttctggcgct
    30661 gaaaatgatc aacgacattc tgcacctgca aattagctgg ggtggatggg cgctggccgc
    30721 cggattgccg ggcatcatta tgctgctggt caccccgctg gtgatttaca ccatgtatcc
    30781 accagaaatt aagaaggtgg ataacaaaac catcgctaaa gcgggccttg ccgaactggg
    30841 accgatgaaa atccgcgaaa aaatgctgct cggtgtcttt gtgctggcgc tgctgggctg
    30901 gattttcagt aagtctctgg gggttgatga atcttcagtg gctattgttg ttatggcgac
    30961 catgctgctg ctgggtatcg ttacctggga agacgtggtt aaaaataaag gcggctggaa
    31021 taccttaatc tggtacggcg gtattatcgg cttaagctcc ttattatcga aagttaaatt
    31081 tttcgaatgg ttagctgaag tctttaaaaa taacctggca tttgatggtc acggtaacgt
    31141 tgctttcttc gttattattt tcctcagcat catcgtgcgt tatttcttcg cttccggtag
    31201 tgcctatatc gttgctatgt taccggtatt tgccatgctg gcgaacgtct ccggcgcacc
    31261 gttaatgtta accgcgctgg cactgttgtt ctccaactcc tatggcggca tggttactca
    31321 ctatggcggc gcggcaggtc cggtcatctt tggcgtgggt tataacgata ttaaatcctg
    31381 gtggttggtc ggtgcggtac tgacgatatt aaccttcctg gtgcatatca ccctcggcgt
    31441 gtggtggtgg aatatgctga tcggctggaa catgctgtaa atatactcgt catacttcaa
    31501 gttgcatgtg ctgcgtctgc gttcgctcac cccagtcact tacttatgta agctcctggg
    31561 gattcactcg cttgtcgcct tcctgcaact cgaattattt agagtatatc catttatttt
    31621 cttgctgcgc acttcacggt gcgcagatat ctggagcatt tgatgatcaa gttatctgaa
    31681 aaaggcgtgt ttctcgccag taataacgaa ataattgccg aagaacattt caccggcgaa
    31741 attaaaaaag aagaagcaaa aaaaggcact attgcctggt ctattctctc ttctcataat
    31801 acgtccggaa atatggataa acttaaaatt aagtttgatt cattagcctc tcacgatatt
    31861 acctttgttg gtattgtaca gaccgctaaa gcgtccggta tggaacgttt cccgctgccg
    31921 tatgtgctga ccaactgcca taactcactc tgcgccgtcg gcggcaccat taacggtgat
    31981 gaccatgttt ttggtttatc ggcagctcag cgttatggcg gtatttttgt gcctccgcat
    32041 attgcggtca tccatcaata tatgcgtgag atgatggcag gtggcggcaa aatgatcctc
    32101 gggtcagaca gtcacacccg ttacggtgca ttagggacaa tggcagtcgg tgagggcggc
    32161 ggcgagttgg taaaacaatt gcttaatgac acctgggata tcgactatcc gggagttgtt
    32221 gcggtgcatc tgaccggaaa accagcgccg tatgtggggc cgcaggatgt ggcgttggct
    32281 atcatcggtg ccgtgttcaa aaacggctac gtcaaaaaca aagtgatgga attcgtaggt
    32341 cccggtgttg ctgcgctctc taccgatttc cgtaacagcg ttgacgttat gaccactgaa
    32401 acgacctgtt taagttctgt ctggcaaacc gatgaagaag tccataactg gctggcgctg
    32461 cacggtcgcg gccaggatta ctgccagctt aaccctcaac cgatggcgta ctacgatggc
    32521 tgcatcagcg ttgatttaag cgccatcaaa ccaatgattg cgctgccgtt ccacccgagc
    32581 aacgtgtatg aaatcgacac actgaaccag aacttgaccg acattctgcg tgagattgaa
    32641 attgagtccg aacgcgtggc gcacggtaaa gccaaactct cgctgctgga caaagtggaa
    32701 aatggtcgcc tgaaagtgca gcaggggatt atcgcgggct gttctggcgg taactacgaa
    32761 aacgtcatcg cggcggcgaa tgcactgcgc ggtcaatcct gtggcaatga caccttctcg
    32821 ctggcggttt acccgtcatc acagccggtg tttatggatc tcgccaaaaa aggtgtggta
    32881 gcagatttga ttggcgcagg cgcaatcatc agaaccgcgt tctgcggccc atgctttggc
    32941 gcgggcgata cgccaatcaa caacggtttg agtattcgcc acaccacgcg taacttcccg
    33001 aaccgcgaag gctctaagcc agctaatggg cagatgtcag cggtggcgtt gatggacgct
    33061 cgttctatcg ctgcgactgc ggcaaacggt ggctatttaa cctctgccag cgaactcgat
    33121 tgctgggaca acgtgccgga gtacgccttc gatgtaacgc cgtataaaaa tcgcgtctat
    33181 caaggctttg tgaaaggggc ggcccagcaa ccgctgattt acggaccgaa cattaaagac
    33241 tggccggaat tgggtgcgct gactgacaat atcgtcctca aagtgtgctc gaagatcctc
    33301 gacgaagtga ccaccaccga cgaattgatt ccttccggtg aaacctcttc ttatcgttca
    33361 aatccgattg gtctggcgga gtttaccctg tcacgccgcg atccaggtta tgttggcagc
    33421 agtaaagcga ctgctgagct ggaaaatcag cgtctggcgg ggaatgtcag cgagctgaca
    33481 gaggtgtttg cgcgcattaa gcagattgct ggtcaggagc atattgatcc gctgcaaact
    33541 gaaattggca gcatggtcta tgcggtgaaa ccaggcgatg gttctgcgcg tgaacaggcg
    33601 gcgagttgtc agcgtgtgat tggcggtctg gcgaatattg ccgaggagta cgcgactaaa
    33661 cgctatcgtt ctaacgtcat caactggggg atgttaccgc tgcagatggt ggaagcgcca
    33721 acctttgaag tgggggatta catttacatc cctggcatta aagcggcgct ggataatccg
    33781 ggtacgacgt ttaaaggtta tgtgatccat gaagatgcgc cggtaacgga aattacgctc
    33841 tatatggaaa gtctgactgc tgaagagcgc gagattatca aggcgggtag tttgattaac
    33901 ttcaataaaa accgtcagat gtaaaaagcg ccatgtgaat gtaggtcgca ttcggcactt
    33961 attgtcggat gcgatgctgg cgcatcttat ccgacctaca aatcgcatcg aaccgtaggc
    34021 cggataaggc gtttacgccg catccggcaa atagttaata gctcttactt cttcgcctct
    34081 gcaaccactt tgctacccac gccgcggtta ttgtattccc acatgcggtt gtagttagtg
    34141 tcattcagat tgcgctgtat ttcgtcgcta tcgtcaacgc tgccggtgtt acccgcaaac
    34201 ggacgattag agatcaccgc atcgccccac ggtttagccg tgttaaaacc ttcgttgatg
    34261 gcgctatcac ggatcaccac ctgaccgttg gtattggcat caacatccag cgagcggccc
    34321 agttgtgcca cgccatcacc ggaagcattg aaacggctgt ttacggcgag gaaaccgtag
    34381 taaatgttgg acagcgtagc cggtgcaaac acatacgctt cttgctgagt acgtgagttc
    34441 accacgcgga attcggtgtt atcgaacacc actgcgccgc gaccagaaac gatatccaca
    34501 tccccttcaa tgtagctgtt ggtcaccagc gtacgcggct gacgattcgt ttccagacgg
    34561 ttctgcacac cgctgttggt gacaaagaag gtgttctgac gaccgagaat gttaacgttg
    34621 ttaatctgta cctggtcacc gtcagtacgc agtgccaccg ccggatggtt acccgcatct
    34681 acgctatcac ccagcgtgtt ttcgatggtc aggttttgca gttgcaggcc attgttttgt
    34741 gaccagaaga ccgcagaaca cagaacaccg atactgtcgc tgcgtttgct ctggcagcta
    34801 tcgtacatat accacgctgg tttacctggc atatatttgc cgcgcgggtt gacgtcgtga
    34861 cgccagtcgg cagggctcat gcccccatca agggaaagcc caatcttcac atcaatcggt
    34921 ttttcacctg taccgtacag agtaattcca cctggagcag cagggacata caccgttccc
    34981 tgatactcac caggcatcac ggcaatatac tggcgcttgt tggtgcgctt gataattgcc
    35041 gcatctaccg ccgcctgaat cgtggtatgc gttacacctt gagtacccgc cgggccgaca
    35101 acaaagtcag gttgcgcagg cagggttatc ggggacggat tccacgctgc agcacctggt
    35161 gtcagggatg caaaatagtg ttgagcatcg aaattctgcg cttcttttgc cgacagaatc
    35221 gggcgagaag aggtaccagg cgcagtttga tcagaaggac gttgatcggg tggtgttgag
    35281 ctacaggcgg tcagcgtcac gccaaaagcc aatgccagcg ccagacggga aactgaaaat
    35341 gtgttcacag gttgctccgg gctatgaaat agaaaaatga atccgttgaa gtctgctttt
    35401 ttatactaag ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
    35461 actatcagtc aaaataaaat cattatttga tttcaatttt gtcccactcc ctgcctctgt
    35521 catcacgata ctgtgatgcc atggtgtccg acttatgccc gagaagatgt tgagcaaact
    35581 tatcgcttat ctgcttctca tagagtcttg cagacaaact gcgcaactcg tgaaaggtag
    35641 gcggatcccc ttcgaaggaa agacctgatg cttttcgtgc gcgcataaaa taccttgata
    35701 ctgtgccgga tgaaagcggt tcacgacgag tagatgcaat tatggtttct ccgccaagaa
    35761 tctctttgca tttatcaagt gtttccttca ttgatatccc gagagcatca acatgcaatg
    35821 ttgtagggat ggcaattttt acgcctgttt tgctttgttc gacataaaga tatccatcta
    35881 cgatatcaga ccacttcatt tcgcataaat caccaactcg ctgcccggta acaacagcca
    35941 gttccattgc aagtctgagc caacatggtg atgattctgc tgcttgataa attttcaggt
    36001 attcgtcagc cgtaagtctt gatctcctta cctctgattt tgctgcgcga gtggcagcga
    36061 ccgggtttgt tgttatatgg ccttcagcta ttgcctctcg gaatgcatcg ctcagtgttg
    36121 atctgattaa cttggctgac gccgccttgc cctcgtctat gtatccattg agcattgccg
    36181 caatttcttt tgtggtgatg tcttcaagtg gagcatcagg cagccccctc cttattgctt
    36241 taattttgct catgtaattt atgagtgtct tctgcttgat tcctctgctg gcgaggattt
    36301 tttcgtagcg atcaagccat gaatgtaacg taacagaatt atcactgttg attctcgctg
    36361 tcagaggctt gtgtttgtgt cctgaaaata actcaatatt ggcctgtatt gcttcagtga
    36421 ttgctatcct cctgtctcgg cctaaaccaa actctttacc cgtccttggg tccctgtagc
    36481 agtaatatcc attgtttctt atataaaggt tagggggtaa atcccggcgc tcatgacttc
    36541 gccttcttcc catttctgat cctcttcaaa aggctacctg ttactggtcg atttaagtca
    36601 acctttaccg ctgattcgtg gaacagatac tctcttccat ccttaaccgg aggagggaat
    36661 atcctgcatt cgcgcaccca tcgacgaact gtttcaaggc ttcttgggcg tcgctggcgt
    36721 gcgttccact cctgaagtgt caagtacatc gcaaagtctc cgcaattaca cgcaagaaaa
    36781 agccgccatc aggcggcttg gtgttctttc agttcttcaa ttcgaatatt ggttatgtct
    36841 gcatgtgcta tctgcgccca tatcatccag tggtcatagc agtcattgat gttctctgct
    36901 tcgataactc tgttgaatgg ttctccattc cattcacctg tgactcggaa gtgcatttat
    36961 catctccata aaacaaaacc cgccgtagcg agttcagata aaagaaatcc tcgtcagtgc
    37021 gaggatgctg ttcattgctg ctatacactt ttttgctctc aacgtaagcg gtagctcatt
    37081 ctgttgggtt ggtgcagttg cttttaggaa atgctattta ccccttaaac gtcggctgaa
    37141 agagctaaaa tccatgcaaa aaatttacgc aattttgtgt attattgtgc agtaagtaat
    37201 gagctatttt ctgcgcaaaa aatggatggt aaatttgtcc ggggcaggaa aaattttatg
    37261 ggcgctaaac atgaaaaaag attcgtatcc ttatttgatt tgcatgacag ttttaggcct
    37321 gatctttatt ttccttttct tctggtggcg ggcagatatt tacagggtca cgtttcttaa
    37381 tcagagtata tcccactatt acattctgtt tagcatggga atagcttttc tgttatctct
    37441 gttttgggtt aagaagggga tagtaaaaca aagcggctgg aagagtctgt cagcatacct
    37501 taaggtttat gcagggatgt gcatatttgc tggatttttt ctgattatac cccttacaac
    37561 actaacttat tttttgcctg gagagacatc gtcttatgtt gcaccgtatc ggtatacttc
    37621 cggtagttca aaaagttgtt ctggagctga ggtggatgac cccgatctac atgagaatat
    37681 tcgcatttgc tatccgtatg gcaattatga gtacgataat attatctatg ttgaaaagaa
    37741 aattaataca ttaggtgcgg tagtaacata tgcac
//
LOCUS       DBFIFM010000018        37338 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.018, whole genome shotgun sequence.
ACCESSION   DBFIFM010000018 DBFIFM010000000
VERSION     DBFIFM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.018"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..321
                     /gene="ytfI"
                     /locus_tag="N7C74_000867"
     CDS             <1..321
                     /gene="ytfI"
                     /locus_tag="N7C74_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HGU2430793.1"
                     /translation="DDNFAIVIESLGFALKIYGGDERFRNGSSVVLEDEDYSLIYNFL
                     VNAGCQQVELAEQVDAIVSANLAADSDITKEKICEKYKSTIEAFKKEQLALPVLVRRK
                     NSET"
     gene            complement(384..938)
                     /gene="ytfJ"
                     /locus_tag="N7C74_000868"
     CDS             complement(384..938)
                     /gene="ytfJ"
                     /locus_tag="N7C74_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HGU2430794.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            1263..1469
                     /gene="ytfK"
                     /locus_tag="N7C74_000869"
     CDS             1263..1469
                     /gene="ytfK"
                     /locus_tag="N7C74_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HGU2430795.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(1548..2891)
                     /gene="paeA"
                     /locus_tag="N7C74_000870"
     CDS             complement(1548..2891)
                     /gene="paeA"
                     /locus_tag="N7C74_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HGU2430796.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(3214..3852)
                     /gene="msrA"
                     /locus_tag="N7C74_000871"
     CDS             complement(3214..3852)
                     /gene="msrA"
                     /locus_tag="N7C74_000871"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HGU2430797.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLHGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            4058..5791
                     /gene="tamA"
                     /locus_tag="N7C74_000872"
     CDS             4058..5791
                     /gene="tamA"
                     /locus_tag="N7C74_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HGU2430798.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            5788..9567
                     /gene="tamB"
                     /locus_tag="N7C74_000873"
     CDS             5788..9567
                     /gene="tamB"
                     /locus_tag="N7C74_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HGU2430799.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGILNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELTVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTLVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTEEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            9570..9911
                     /gene="ytfP"
                     /locus_tag="N7C74_000874"
     CDS             9570..9911
                     /gene="ytfP"
                     /locus_tag="N7C74_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HGU2430800.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            10123..10374
                     /gene="chpS"
                     /locus_tag="N7C74_000875"
     CDS             10123..10374
                     /gene="chpS"
                     /locus_tag="N7C74_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HGU2430801.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            10368..10718
                     /gene="chpB"
                     /locus_tag="N7C74_000876"
     CDS             10368..10718
                     /gene="chpB"
                     /locus_tag="N7C74_000876"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HGU2430802.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(10798..11328)
                     /gene="ppa"
                     /locus_tag="N7C74_000877"
     CDS             complement(10798..11328)
                     /gene="ppa"
                     /locus_tag="N7C74_000877"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HGU2430803.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            11638..12594
                     /gene="ytfQ"
                     /locus_tag="N7C74_000878"
     CDS             11638..12594
                     /gene="ytfQ"
                     /locus_tag="N7C74_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HGU2430804.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            12734..14236
                     /gene="ytfR"
                     /locus_tag="N7C74_000879"
                     /pseudo
     CDS             12734..14236
                     /gene="ytfR"
                     /locus_tag="N7C74_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
     gene            14250..15272
                     /gene="ytfT"
                     /locus_tag="N7C74_000880"
     CDS             14250..15272
                     /gene="ytfT"
                     /locus_tag="N7C74_000880"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HGU2430805.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIIT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            15259..16254
                     /gene="yjfF"
                     /locus_tag="N7C74_000881"
     CDS             15259..16254
                     /gene="yjfF"
                     /locus_tag="N7C74_000881"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HGU2430806.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(16287..17285)
                     /gene="fbp"
                     /locus_tag="N7C74_000882"
     CDS             complement(16287..17285)
                     /gene="fbp"
                     /locus_tag="N7C74_000882"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HGU2430807.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYSTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFICEFPDA"
     gene            17461..18834
                     /gene="mpl"
                     /locus_tag="N7C74_000883"
     CDS             17461..18834
                     /gene="mpl"
                     /locus_tag="N7C74_000883"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HGU2430808.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(18990..19541)
                     /gene="yjgA"
                     /locus_tag="N7C74_000884"
     CDS             complement(18990..19541)
                     /gene="yjgA"
                     /locus_tag="N7C74_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HGU2430809.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            19635..20987
                     /gene="pmbA"
                     /locus_tag="N7C74_000885"
     CDS             19635..20987
                     /gene="pmbA"
                     /locus_tag="N7C74_000885"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HGU2430810.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            21171..21557
                     /gene="cybC"
                     /locus_tag="N7C74_000886"
     CDS             21171..21557
                     /gene="cybC"
                     /locus_tag="N7C74_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HGU2430811.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNSYHKKYR"
     gene            complement(21602..22066)
                     /gene="nrdG"
                     /locus_tag="N7C74_000887"
     CDS             complement(21602..22066)
                     /gene="nrdG"
                     /locus_tag="N7C74_000887"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HGU2430812.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(22256..24394)
                     /gene="nrdD"
                     /locus_tag="N7C74_000888"
     CDS             complement(22256..24394)
                     /gene="nrdD"
                     /locus_tag="N7C74_000888"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HGU2430813.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDISEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(24788..26443)
                     /gene="treC"
                     /locus_tag="N7C74_000889"
     CDS             complement(24788..26443)
                     /gene="treC"
                     /locus_tag="N7C74_000889"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HGU2430814.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVALNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQLGQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            complement(26493..27914)
                     /gene="treB"
                     /locus_tag="N7C74_000890"
     CDS             complement(26493..27914)
                     /gene="treB"
                     /locus_tag="N7C74_000890"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HGU2430815.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(28033..28980)
                     /gene="treR"
                     /locus_tag="N7C74_000891"
     CDS             complement(28033..28980)
                     /gene="treR"
                     /locus_tag="N7C74_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HGU2430816.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPANLS"
     gene            29165..29218
                     /gene="mgtL"
                     /locus_tag="N7C74_000892"
     CDS             29165..29218
                     /gene="mgtL"
                     /locus_tag="N7C74_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HGU2430817.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            29359..32055
                     /gene="mgtA"
                     /locus_tag="N7C74_000893"
     CDS             29359..32055
                     /gene="mgtA"
                     /locus_tag="N7C74_000893"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HGU2430818.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(32261..32647)
                     /gene="ridA"
                     /locus_tag="N7C74_000894"
     CDS             complement(32261..32647)
                     /gene="ridA"
                     /locus_tag="N7C74_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HGU2430819.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(32720..33181)
                     /gene="pyrI"
                     /locus_tag="N7C74_000895"
     CDS             complement(32720..33181)
                     /gene="pyrI"
                     /locus_tag="N7C74_000895"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HGU2430820.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(33194..34129)
                     /gene="pyrB"
                     /locus_tag="N7C74_000896"
     CDS             complement(33194..34129)
                     /gene="pyrB"
                     /locus_tag="N7C74_000896"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HGU2430821.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(34133..34267)
                     /gene="pyrL"
                     /locus_tag="N7C74_000897"
     CDS             complement(34133..34267)
                     /gene="pyrL"
                     /locus_tag="N7C74_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HGU2430822.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(34548..34943)
                     /gene="yjgH"
                     /locus_tag="N7C74_000898"
     CDS             complement(34548..34943)
                     /gene="yjgH"
                     /locus_tag="N7C74_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGU2430823.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPTWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(35074..35787)
                     /gene="bdcA"
                     /locus_tag="N7C74_000899"
     CDS             complement(35074..35787)
                     /gene="bdcA"
                     /locus_tag="N7C74_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU2430824.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            35858..36451
                     /gene="bdcR"
                     /locus_tag="N7C74_000900"
     CDS             35858..36451
                     /gene="bdcR"
                     /locus_tag="N7C74_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGU2430825.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLVDILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARLHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGEAIKTILKE"
     gene            36596..37048
                     /gene="tabA"
                     /locus_tag="N7C74_000901"
     CDS             36596..37048
                     /gene="tabA"
                     /locus_tag="N7C74_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HGU2430826.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            37171..>37338
                     /locus_tag="N7C74_000902"
     CDS             37171..>37338
                     /locus_tag="N7C74_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2430827.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAGGQD
                     NTRNILNPKTFL"
BASE COUNT         8974 a         9303 c        10038 g         9023 t
ORIGIN      
        1 gatgataatt ttgccatagt catagaatct ttgggctttg cgcttaaaat ctatggaggg
       61 gatgaacgtt ttcgtaatgg ttcctctgtt gtcttggagg atgaagatta ctctcttatt
      121 tataattttc tggttaatgc gggttgccaa caagtcgaat tagccgaaca agtggatgcg
      181 atagtaagtg cgaatctggc cgcagatagt gatattacca aagaaaagat ttgcgagaaa
      241 tataaatcga ccattgaggc gtttaaaaaa gagcaactag cgttaccagt attagttcgg
      301 cgtaagaact cagagactta attcccctat cccatagata acgatagggg aaaattattt
      361 ttgcagaatt tatgttattg ccattatttg agcaactttt gcagcaggtc catcacttgc
      421 tgcacctctt cctgagtgag cgccccgtct ttggcccatt gcacgcgacc gtctttatcc
      481 agcaccacta cagcggaact ttcttcatcc agctgccagg caccgcgcgc gacgccattg
      541 ctatcgacaa taaactgcga ccagggataa agctttttat tactctccag actgctgcgc
      601 acaaacatgc cggaacccgg aatcgcgtcg tcggtgttaa caatggtggt ggtctggtaa
      661 cgatcgtgcg gtaacttcgc tgatttaatc gcttcaatca gcgtcgcgtt tttctctttt
      721 gcagaggtgc gaccagcaat atgttgcagt actcgcactt ttcccactaa ctgcgcgctg
      781 ttccaggttt tgtagctaaa ctgatcttta tcaagcacca actcgccccg atcggtaatg
      841 ccaatcggcg gcactcgctg accggtttcg aactgatgtg cggaagccat catcggcaac
      901 agcaggcagg tgagtgccag aatcttgcgt agggtcatgg tgtttccttc ttatgatatg
      961 caggtgatcc gaccacttgg gtctgagttt taatcatatg tgctatttat cgaatttccc
     1021 gcaagtgtga tgccagtttg cggtcaagcg cacaaatcat atgaaaaatg aatgcttata
     1081 ctgaagaccg cgcttcggta aaaagataat tctgaataat tgtaaccttt aggtaaaaaa
     1141 agttatacgc ggtggaaaca ttgcccggat agtctatagt cactaagcat taaaatttgc
     1201 gcctcataat agttgggccg attgtggcac cgcacaggcg taatactcag caggagataa
     1261 caatgaaaat tttccaacgg tacaacccac ttcaggtggc gaagtacgta aagatcctgt
     1321 tccgtggacg attatacatc aaggacgttg gcgcttttga attcgataag ggtaagattc
     1381 ttatcccaaa agtgaaggat aagctgcatt tgtcagtgat gtccgaagtt aaccgtcagg
     1441 ttatgcgtct gcaaacagag atggcttaac caaagtgcta tgcagtaaaa agtgctatgc
     1501 agtaataaga cggctcctga ttcaggagcc gttgatgttt atgggggtta cgcgacgctt
     1561 tcttctttat ctttagcgtc aggcagtttt ggcgaaaggg ctgtggcctt gctgtcgatc
     1621 cgggtcacca gcagctggtc gatgcggtag ttatcgatat ccaccacttc aaatttgtag
     1681 ccggcgaatt tcaccgaatc ggtgcgtttc gggatcttac gcagcataaa catcataaag
     1741 ccgccgatgg tttcgtagtt gcccgactgc gggaactcgt caatatccag cacgcgcatg
     1801 acgtcgtcaa tcggagtgcc gccgtcaatc agccatgagt tctcatcacg ggcgacaatc
     1861 tgctcttcca gtccctgacc gaccagatcg cccatcagcg tggtcatcac gtcgttgagg
     1921 gtgatgatcc ccactaccag cgcgtactcg ttcatgatca ccgcgaagtc ttctcctgcg
     1981 gttttaaaac tttccaacgc ctctgaaagg gttaacgtat ccggcacaat cagcgtgttg
     2041 cgaatttgta cgccgctgtt cagtgccagg ctttggttag ccagcacgcg gttcagcagg
     2101 tctttagaat cgacatagcc gatgatgtgg tcaatatctt cattacagac gaggaactta
     2161 gagtgcggat gttccgccac cttatttttc agactttgct catcttcgtg gagatcaaac
     2221 caaatcacgt tttcacgcgg tgtcattgaa gacggaacgg tacgggattc cagctcaaag
     2281 acgttttcaa tcagctcgtg ttcctgttta cgtaacacgc ccgccagcgc accggcttcc
     2341 actaccgcgt agatgtcatc agaagtgatg tcatctttac gtaccattgg cagtttgaaa
     2401 atacggaaga ttatgttcgc caggccgttg aagaaccaca ccagcggggt gcaaacgtac
     2461 aggcagaagc gcatcgggtt gatgatacgc aaagccacag cttctggcgc aatcataccg
     2521 atgcgtttcg gggttaaatc cgcaaacagg ataaacatgc cagtcactaa cgagaaagag
     2581 agaataaagc tcagttgctc agagagctct gccgacatat agcgggagaa caggctgtga
     2641 aaagctggag aaaatgccgc atcaccgacg ataccgccga gaatcgccac tgcgttcaga
     2701 ccgatttgga ccacggtaaa gaacatgccg ggattttcct gcatattcag aacgcgttgg
     2761 gcatttatat tgccttcatc agccagcagt ttaagtttga ttttgcgtga ggcggcaagt
     2821 gagatctcag acatcgagaa gaacgcactt acagcgatca agcagagtat gactaaaata
     2881 ctgtttaaca tatcttatcc gacctttcag gccagatcct cggaagggga agttgattaa
     2941 tttgtgtgaa atacacattg aaagccgatt cgaagaaagt gaaccggcat tttcagtggc
     3001 tagtatagcg taaggtactg taaagccgcc agagggttaa aattcagaca gctgaaaaat
     3061 gcaaaactgc ctgatgcgct atgccgatca ggcctacgca gtccttgcaa tatattgaat
     3121 ttggacgatt ttgtaggcct ggcaaggcgt ttacgccgca tccggcataa acaaaacgca
     3181 ctttatcaac aatctacatt tgtacccgta acgctatgct tccggtggca gacagacgcc
     3241 aattccgcca attccacagt aaccgtacgg gtttttatgc agatattgct ggtggtcatc
     3301 ttcggcataa taaaacggtg tggcgttagc gatttccgtg gtgatgtgac gatcgtcatc
     3361 ggcggcaagc atcgccgcct gaaaacgttc cagactggcg cgagctgcgg catcctgttc
     3421 tggggtcagc ggataaatcg ctgaacgata ctgcgtgccg tggtcattgc cctgacgcat
     3481 tccctgggcg ggatcgtgat tctcccaaaa tacctgtagc aactgctcat agctgatgac
     3541 ggaaggatcg taaacaatgc gtaccgcttc ggcatgacca gtatcaccgg agcacacttc
     3601 ccgataagtc ggatttggcg tatagccgcc ggtataacct gcggcggtgc tgtaaacgcc
     3661 gtgtaactgc cagaacagac gctccacacc ccagaaacaa cccatcgcaa aaatggcaat
     3721 ctccattccg tcaggtacat tggtcattga gtgaccgtta accgcatgca gcgtggctac
     3781 gggcatcggg gtgttacgtc caggcagggc atcggcgggg gaaaccagat gctttttatc
     3841 aaataaactc atggtgtcgc tctcccgaaa atcggtcatt ggggttaagg ttgtaacaag
     3901 aggcgtattt gcacacaata accaccgtga ataggtctaa agtaaaacat aagaaatatt
     3961 tggggtttag tctgcttttt aatccatatt actggatttt tgttaagccg tttaacggcg
     4021 ttccaggggc aggaaaaaag gatattcagg agaaaatgtg cgctatatcc gacagttatg
     4081 ctgtgtaagc ttactctgct taagcggatc tgccgtcgcc gcgaacgtcc gtctacaggt
     4141 cgaggggtta tcgggacagc tggaaaagaa cgttcgtgcg cagctttcta cgattgaaag
     4201 tgatgaagtg acgccagacc gtcgctttcg cgcacgcgtc gatgatgcta tccgcgaagg
     4261 tctgaaagcg ctgggttatt accagccgac cattgaattt gatctccgtc caccgccaaa
     4321 gaaagggcgg caggtattga tcgccaaagt cacgccaggc gtgccggtgt taattggcgg
     4381 caccgatgtg gtattgcgcg ggggcgcgcg gaccgataaa gactatttga aattgctcga
     4441 tactcgcccg gctattggca cggtactgaa ccagggcgat tatgaaaatt tcaaaaagtc
     4501 cttaaccagc attgcgttgc gtaaaggtta tttcgatagc gaatttacca aagcgcagct
     4561 gggcattgcg ctcggcctgc ataaagcctt ctgggatatt gattataaca gtggcgaacg
     4621 ttaccgcttt gggcatgtga cctttgaagg atcacaaatc cgcgatgaat acctgcaaaa
     4681 tctggtgccg tttaaagagg gcgatgagta cgaatcgaaa gatctggcag aactgaaccg
     4741 ccgactttct gctaccggct ggtttaactc ggtggtggtg gctccacaat ttgataaagc
     4801 gcgcgaaacg aaagtattac cattgacggg cgtggtttcg ccgcgaacag aaaacactat
     4861 cgaaaccggg gtcggttact ctacggacgt gggaccgcgc gtgaaagcga cgtggaaaaa
     4921 accgtggatg aactcatacg gtcacagtct gaccaccagt accagtattt ccgcgccgga
     4981 acagaccctc gacttcagct ataaaatgcc gctgctgaag aatccactgg aacaatatta
     5041 tttggtgcag ggcggtttta agcgcactga cctgaacgat accgagtctg actccactac
     5101 gctggtggct tctcgctact gggatctctc cagcggctgg cagcgtgcca ttaacctgcg
     5161 ctggagtctc gaccacttta ctcagggtga aattaccaac accacgatgc tgttttatcc
     5221 tggggtgatg attagccgca cgcgttctcg tggtggcctg atgccaacct ggggcgactc
     5281 gcaacgctac tctatcgact actccaacac ggcctggggt tcagatgtcg atttctccgt
     5341 tttccaggcg cagaacgtct ggatccgcac actgtacgat cgccatcgtt ttgtgacacg
     5401 cggcacgctg ggctggattg aaaccggtga tttcgacaaa gtaccgccgg atctgcgttt
     5461 cttcgccggg ggcgatcgca gtattcgcgg ctacaaatac aaatctatcg ccccgaaata
     5521 cgctaacggt gacctgaaag gggcctcgaa gttgataacc ggatcgctgg aataccagta
     5581 caacgtgacc ggaaaatggt ggggcgcggt gtttgtcgat agtggcgaag cggtaagcga
     5641 tattcgccgc agcgacttta aaaccggtac cggggtcggc gtacgctggg aatcgccggt
     5701 cgggccaatc aaactcgatt ttgccgtacc ggtcgcggat aaagacgaac acgggttaca
     5761 gttttacatc ggtctggggc cagaattatg agtttatgga aaaaaatcag cctcggcgtg
     5821 gttatcgtta tcttactgtt gctgggatcg gtggcgtttc tggtgggcac caccagcggc
     5881 ctgcatctgg tatttaaagc ggcggatcgc tgggtgccag gactggatat tggcaaggtc
     5941 accggcggct ggcgcgatct caccttgtct gacgttcgtt atgagcagcc aggcgtggcg
     6001 gtaaaagcgg gtaatctgca tctggcggtc ggtcttgagt gcctgtggaa cagcagtgtt
     6061 tgtattaatg acttggcgct gaaagacatt caggtcaaca tcgacagtaa aaaaatgcct
     6121 ccttctgaac aggttgaaga agaggaagat agcggtccgc tggatctctc cacgccgtat
     6181 cccatcaccc tgacacgggt ggcactggac aacgtcaaca tcaagattga tgacaccacg
     6241 gtatcggtga tggacttcac ctccggcctg aactggcagg agaaaaccct gaccctgaaa
     6301 ccgacgtcgc tgaaaggcct gctgattgct ctgccgaaag tggcggaagt ggcgcaggaa
     6361 gaagtggtcg aaccgaaaat tgaaaatccg cagccggagg aaaagcctct cggcgaaaca
     6421 ctcaaagatc tcttttctcg cccggtattg ccggaaatga ccgacgtgca tctgccactc
     6481 aacctgaaca ttgaagagtt taagggagag caactgcgcg tgacgggcga tacggacatt
     6541 accgtgcgca ccatgctgct gaaagtgagc agcattgacg gcaatactaa actggacgcc
     6601 ctggatatcg attccaatca agggatcctc aacgccagcg gcacggcgca gctgtcagac
     6661 aactggccgg tggatatcac cctcaacagc acactgaacg tggagccgtt gaaaggtgaa
     6721 aaggtgaagc tgaaagtggg cggcgcgctg cgcgaacagc tggagattgg cgttaatctt
     6781 tccggtccgg tggatatgga tttacgcgcc cagacgcgac tggcggaagc cggattgccg
     6841 ctcaacgtgg aagtgaacag caaacagatt tactggccgt tcactggtga gaagcagtat
     6901 caggcggatg atctgaaact gaaacttacc ggcaaaatga ccgattacac gctctctatg
     6961 cgtacggcag tgaagggact ggagatcccg ccagccacca ttacccttga tgccaaaggt
     7021 aatgaacagc aggtcaatct cgacaaactc accgtcgcgg cgctggaagg gaaaactgaa
     7081 ctcaaggcgt tgcttgactg gcagcaggcc attagttggc gcggtgagct aacgcttaac
     7141 ggcattaaca ccgccaaaga gatcccagag tggccgtcga aactcaatgg cttgattaaa
     7201 acccgcggta gcctgtacgg cggcacctgg cagatggagg tgccagaact gaagcttacc
     7261 ggtaacgtca aacagaacaa agtgaacgtt gacggcacgc tgaaaggcaa cagttatatg
     7321 cagtggatga tcccagggct tcatctggaa ctggggccaa acagtgccga agtgaaaggc
     7381 gagctggggg taaaagatct caatcttgat gccaccatca acgcgccggg gctggataac
     7441 gcgctgccgg ggcttggcgg tacagcgaaa gggctggtga aagttcgcgg cacggtggaa
     7501 gcgccacaac tactggcgga catcaccgcg cgcggcctgc gctggcagga gctaaccgtg
     7561 gcgcaggttc gcgtggaagg cgatatcaaa tccaccgatc agattgcggg taaactcgac
     7621 gtgcgcgttg agcaaatttc acagccggat gtaaatatca acctcgtcac cctgaatgcc
     7681 aaaggcagcg aaaaacagca cgagttacag ttgcggattc agggcgagcc ggtttccggg
     7741 cagcttaatc tggcaggaag ttttgatcgc aaagaagaac gctggaaggg aactcttagc
     7801 aatacccgct tccagacgct ggtcggcccg tggtcgctga cccgcgatat tgcgctggat
     7861 taccgcaata aggagcaaaa aatcagcatc gggccacact gctggcttaa cccgaatgcg
     7921 gaactgtgcg tgccgcaaac tatcgatgcg ggggccgaag ggcgtgcggt ggtgaatctc
     7981 aaccgcttcg accttgccat gctgaaaccg tttatgccag aaaccactca ggccagcggt
     8041 atcttcacgg gtaaagcgga cgttgcctgg gacaccaccg aagaggggct gccgcagggc
     8101 agtatcaccc tttcggggcg taacgtgcag gtaacgcaaa ccgtcaacga tgcggcgctg
     8161 cccgtggcgt ttcagacact gaatctgacg gcggaattgc gtaacaaccg tgccgaattg
     8221 ggctggacca tccgcctgac caataacggc cagtttgatg gacaggtgca ggtgaccgat
     8281 ccgcaaggcc gccgtaatct tggtggcaac gtcaatatcc gtaacttcaa ccttgcgatg
     8341 ataaacccca tctttacccg tggggaaaaa gcagcgggga tggtgagtgc caacttgcgt
     8401 ctgggtggtg atgtgcaaag cccgcagttg tttggtcagc ttcaggttac gggtgtggat
     8461 atcgatggca actttatgcc gtttgatatg caaccgagcc agcttgcggt caactttaac
     8521 ggtatgcgct cgacgcttgc cggtacagta cgaacccagc agggtgaaat ctacctgaac
     8581 ggtgatgccg actggagcca aattgaaaac tggcgggcgc gggtaacggc gaaaggcagt
     8641 aaagtgcgga tcaccgtgcc gccgatggta cgaatggatg tatcgccaga tgttgtattc
     8701 gaggctacac caaacctgtt taccctcgat ggtcgcgtgg atgtcccttg ggcgcgcatc
     8761 gtggtgcacg atctgccgga aagcgcagta ggcgtctcca gcgatgtggt gatgcttaac
     8821 gataacctgc aaccggaaga gccgaaaacg gcgtcgattc cgattaacag taacctgatt
     8881 gtccacgttg gcaacaatgt gcgcattgac gcctttggcc tgaaagcgcg gctgacgggc
     8941 gatcttaacg tcgttcagga caaacaaggg ctgggcctga acgggcagat caacatccct
     9001 gaagggcgct tccatgccta tggtcaggat ctgattgtgc gtaaaggtga gttactgttc
     9061 tctggcccgc cggatcaacc gtatcttaat attgaagcta ttcgtaaccc ggatgctaca
     9121 gaagacgacg taatcgccgg agttcgcgtc actggtctgg cggacgaacc gaaagcggag
     9181 atcttctctg acccggcgat gtcgcaacaa gctgccttgt cttatttgct acgtggacaa
     9241 gggctggaga gcgatcagag cgacagtgcg gcaatgacct cgatgctgat tggtctgggg
     9301 gttgcgcaaa gtggccagat tgtaggtaaa atcggcgaga cgtttggcgt aagcaattta
     9361 gcgctcgaca cccagggagt aggcgactcc tcccaggtag tggtcagcgg ctatgtattg
     9421 ccaggtctgc aagtgaaata cggcgtgggt atatttgact ctatagcaac actcacgtta
     9481 cgttatcgcc tgatgcctaa gctatatctg gaagccgtgt ctggtgtaga ccaggcactg
     9541 gatttgctct atcagttcga gttttagcaa tgcgaatatt tgtctacggc agtttacgcc
     9601 acaaacaagg caacagtcac tggatgacca atgcccagtt actgggcgat ttcagtatcg
     9661 ataactacca gttgtatagc ctgggccact atccaggcgc agttccgggg aacggaacgg
     9721 tacacggtga agtttatcgt attgacaacg ccacgctggc cgaacttgat gccttgcgca
     9781 ccaggggcgg tgaatacgcg cgccagttga ttcagacgcc gtacgggagt gcatggatgt
     9841 acgtttatca acgacccgtc gatggattaa agctaattga aagcggcgac tggttagaca
     9901 gggataagta accctatgca tacgccacct tcgggtggcg ttgttttttg cgagacgact
     9961 cgcattctgt tttgtaattc cctcaccttt tgcttttctc tccgagccgc tttccatatc
    10021 tattaacgca taaaaaactc tgctggcatt cacaaatgcg caggggtaaa acgtttcctg
    10081 tagcaccgtg agttatactt tgtataactt aaggaggtgc agatgcgtat taccataaaa
    10141 agatggggga acagtgcagg tatggtcatt cccaatatcg taatgaaaga acttaactta
    10201 cagccggggc agagcgtgga agtgcaggtg agcaacaacc aactgattct gacacccatc
    10261 tccaggcgct actcgcttga tgaactgctg gcacagtgtg acatgaacgc cgcggaactt
    10321 agcgagcagg atgtctgggg taaatccacc cctgcgggtg acgaaatatg gtaaagaaaa
    10381 gtgaatttga acggggagac attgtgctgg ttggctttga tccagcaagc ggccatgaac
    10441 agcaaggtgc tggtcgacct gcgcttgtgc tctccgttca agcctttaat caactgggaa
    10501 tgacgctggt ggcccccatt acgcagggtg gaaattttgc ccgttatgcc ggatttagcg
    10561 ttcctttaca ttgcgaagaa ggcgatgtgc acggcgtggt gctggttaat caggtgcgga
    10621 tgatggatct acgcgcccgg ctggcaaagc gtattggtct ggctgcggat gaggtggtgg
    10681 aagaggcgtt attacgcttg caggcggtgg tggaataagg tgtgtttatt tatcgcgggc
    10741 ataaaaaaac ccttactaac cgaagcccgg cgttcagggt tattacgcca gaagaactta
    10801 tttattcttt gcgcgctcga aggaggcaac gatttcagct ttagcggctt ctgcgttttc
    10861 ccaaccttca actttcaccc acttgccttt ttcgaggtct ttgtagtgct cgaagaagtg
    10921 agcgatttgc gctttcagca gttcaggcag atcgttaacg tctttaatgt gatcgtattc
    10981 tttgctcagc ttgctgtgcg gaaccgcaac cagtttcgca tcttcaccgg cttcgtcggt
    11041 cattttcaga acgccaaccg gacggcaacg gatcacagaa cccggctgca gcgggtacgg
    11101 agttgggacc agtacgtcaa ctgggtcacc gtccagagac agggtgtggt tgatgtaacc
    11161 gtagttgcac ggatagaaca tcgcggtgga catgaagcgg tcaacgaaca gtgcgccgct
    11221 ctctttgtcg atttcgtatt tgatcggatc tgcgttagcc gggatctcaa taacaacgta
    11281 gatgtcttcc ggcagatctt tacccgcagg gacgttgagt aagctcatgt ctgtttcctt
    11341 taaaaatatg tggcaaacaa gtgccgagta ttatagccaa ctcgcgccga atgtcttcgc
    11401 ttgttttcgt cttcattttc ctttttcgcc agttttaaga cggtatgcag agcaggaaaa
    11461 tccataaccg tagccgcatt tttcatagtg agatgaaagc gattacaaac ttgtgattaa
    11521 cgttttattt acttttttga agtgtgatgt aacgcaatcc gttacataac gaattgtcta
    11581 tagttttttc gcgaacatct tttcaccaat aataattact ccgacgagga caaccctatg
    11641 tggaaacgct tacttgtagt ctctgcagtc tcggcagcca tgtcgtctat ggcgttggcc
    11701 gctccattaa ccgtaggatt ttcgcaggtc ggatcggaat ccggctggcg cgccgcagaa
    11761 accaatgtgg cgaaaagtga ggccgaaaaa cgcggaatta cgctgaaaat tgccgatggt
    11821 cagcaaaagc aggaaaacca gattaaagcg gtacgttcct tcgttgcaca aggggtggat
    11881 gcgatcttta tcgctccggt ggtcgcgaca ggttgggaac cggtattaaa agaggcgaaa
    11941 gatgccgaaa tcccggtctt cttgctcgac cgttccatcg atgtgaaaga caaatctctc
    12001 tatatgacca ccgttactgc cgacaacatc ctcgaaggta agttgatcgg tgactggctg
    12061 gtaaaagaag tgaatggcaa accatgcaac gtggtggagc tgcagggcac cgttggggcc
    12121 agcgtcgcca ttgaccgtaa gaaaggcttt gccgaagcca ttaagaatgc gccaaatatc
    12181 aaaatcatcc gctcgcagtc aggtgacttc acccgcagta aaggcaaaga agtcatggag
    12241 agctttatca aagcggaaaa caacggcaaa aacatctgca tggtttacgc ccataacgac
    12301 gacatggtga ttggtgcaat tcaggcaatt aaagaagcgg gcctgaaacc gggcaaagat
    12361 atcctcacgg gttccattga cggtgtaccg gacatctaca aagcgatgat ggatggcgaa
    12421 gcgaacgcca gtgttgaact gacgccgaac atggcgggac ccgctttcga tgcgctggag
    12481 aaatacaaaa aagacggcac catgcctgaa aagctgacgt taaccaaatc caccctttac
    12541 ctgcctgata ccgcaaaaga agagttagag aagaagaaaa atatggggta ttgagggttg
    12601 ctatgcctga tgccgattcg taggccggat aaggcgctcg cgccgcatcc ggcgatggtg
    12661 cactgaagcc tgatgcgacg cttaccgcgt cttatcatgc ctactgggag cacgctttac
    12721 accgggggaa accatgacga ccgaccaaca ccaggagatc ctccgcaccg aaggattaag
    12781 taaatttttc cccggcgtca aagcgttaga caacgttgat ttcagcctgc gccgtggcga
    12841 aatcatggcg ctgctcggtg aaaacggggc gggaaaatca acgctaatca aagcattaac
    12901 tggtgtatac cacgctgatc gcggcaccat ctggctggaa ggccaggcta tctcaccgaa
    12961 aaataccgcc cacgcgcaac aactcggcat tggcaccgtc tatcaggaag tcaacctgct
    13021 acccaatatg tcggtcgctg ataatctatt tataggccgc gaacccaaac gcttcggcct
    13081 tctacgccgc aaagagatgg aaaagcgcgc caccgaactg atggcatctt acggtttctc
    13141 cctcgacgtg cgcgaaccgc tcaaccgctt ttcagtcgcg atgcagcaaa tcgtcgctat
    13201 ttgccgggct atcgatctct ctgccaaagt gctgatcctc gatgaaccca ccgccagtct
    13261 cgacacccag gaagtggagt tactgtttga cctgatgcgt cagttgcgcg atcgcggcgt
    13321 cagcctgatc tttgtcactc actttctcga tcaggtctat caggtcagcg atcggatcac
    13381 cgtcttacgc aacggcagtt tcgtaggctg tcgggaaacg tgctagctac cgcagatcga
    13441 actggtaaaa atgatgctgg ggcgcgagct ggatacccac gcgctacagc gtgccgggcg
    13501 aacattgttg agcgacaaac ccgttgccgc gttcaaaaat tacggcaaaa aaggaacgat
    13561 cgcaccgttt gatctcgaag tacgccccgg cgagatcgtc ggtctggctg gattgctggg
    13621 atcaggacgt accgaaaccg ccgaagtgat cttcggtatc aaacctgctg acagcggcac
    13681 ggcgttgatc aaaggcaaac cgcaaaacct gcgatcgcca catcaggctt cggtacttgg
    13741 cattggcttc tgcccggaag acaggaaaac cgatggcatc atcgctgccg cctcggtgcg
    13801 ggaaaatatc atcctcgctc tctaggccca gcgcggctgg ctacgtccca tttcccgcaa
    13861 agaacagcaa gagattgccg aacgctttat ccgccagctt ggcattcgca caccttcaac
    13921 tgaacaaccg attgaatttc tctccggcgg caatcagcaa aaagtgttgc tttcacgttg
    13981 gctactgacc cgaccgcaat ttctgatcct cgatgagcca acccgcggca ttgatgttgg
    14041 tgcccacgcc gagatcatcc gcctgattga aacgctatgc gccgatggtc tggcgctgct
    14101 ggtgatctcc tccgaactgg aagaactggt gggctatgcc gaccgggtga tcatcatgcg
    14161 cgatcgcaaa caggtggcgg agatcccgct ggcagagctt tccgttccgg cgatcatgaa
    14221 tgccattgcg gcgtaaggag aacagtgtga tgcctcaatc tctcccggac actacgccgc
    14281 caaaaaggcg ctttcgctgg ccaacgggaa tgccgcagct ggcggcactg ttgctggtgc
    14341 tgctggtcga tagcctggtg gccccgcatt tctggcaggt ggtgcttcag gatgggcgtt
    14401 tgttcggtag ccctatcgac attcttaatc gcgccgcccc cgttgcgcta ctggcgattg
    14461 gtatgacgct ggtgatcgca acaggtggga ttgatctctc cgtaggggcg gtgatggcta
    14521 tcgccggagc cacaacggct gcgatgacgg tcgcgggatt cagcctgccg attgttttgt
    14581 taagcgccct gggcaccggc atcctggcgg gattgtggaa cggcatactg gtagcgatcc
    14641 tcaaaattca gccgtttgtc gccactctga tcctgatggt cgccgggcgc ggcgtggcgc
    14701 aactgatcac ctccggacag atcatcacgt ttaactcgcc ggatctctca tggttcggca
    14761 gtggatcgct gttgttcctg ccaacgccgg tcattatcgc ggtgctgacg cttatcctgt
    14821 tctggctgtt gacccgcaaa acggcgctgg ggatgtttat cgaagccgtt ggtatcaaca
    14881 ttcgggcggc aaaaaatgcc ggggtaaaca cgcggatcat cgtcatgctt acctacgtgt
    14941 tgagcgggct gtgtgcggcg attgcgggca ttatcgtggc ggcggatatt cgcggtgccg
    15001 atgccaacaa cgccggatta tggctggagc tggacgccat tctcgcggtg gtgattggcg
    15061 gtggttcgct gatgggcgga cgttttaacc tactgctttc ggtggtgggg gcgctgatta
    15121 ttcaggggat gaacaccgga attttgcttt cgggctttcc gccagagatg aaccaggtgg
    15181 tgaaagcggt ggtggtgctt tgcgtgctga ttgtccagtc gcaacgcttt atcagtctga
    15241 ttaaaggagt gcgtagccgt gataaaacgt aatttgccac tgatgatcac catcggcgtc
    15301 tttgtgctgg gttatctcta ctgcctgacc cagtttcccg gttttgcttc cacaagagtg
    15361 atctgcaata tcctgaccga taatgccttt cttgggataa tcgccgttgg catgaccttt
    15421 gtgatcctct ccggtgggat cgatctctcc gttggttcgg tgatcgcctt taccggcgtg
    15481 tttctggcaa aagtgattgg cgatttcggc ctctcgccgt tgctggcatt tccgctggtg
    15541 ctggtaatgg gctgtgcctt tggcgcattt atggggcttt tgatcgacgc cctgaagatc
    15601 ccggcattta tcattacttt ggcaggaatg ttctttttgc gcggcgtcag ctacctcgtt
    15661 tcggaagagt cgatcccgat aaaccatcct gtttatgaca cgctctcaag ccttgcatgg
    15721 aaaattcctg gcggcggtcg cttaagtgcg atggggctgc tgatgctggc ggtggtggtt
    15781 atcggcatat tcctcgcgca tcgtacccgc tttggtaatc aggtgtacgc cattggcggc
    15841 aatgcgacgt cggcaaacct gatggggatt tccactcgca gcacaactat tcgcatttat
    15901 atgctctcta ccggactggc gacactggcg gggattgtct tctcgattta tacccaggcc
    15961 ggatacgcgc tggcgggcgt aggtgtggaa ctggacgcta tcgcctcagt ggtgattggc
    16021 ggtacgcttt tgagcggtgg cgttggaacg gtattaggga cgctttttgg cgtggcgatt
    16081 cagggactga ttcagactta cataaacttt gatggtacgc tgagctcatg gtggacgaaa
    16141 atcgccatcg gtattttgtt gtttattttt atagcattac agcgtggatt aacggtgctg
    16201 tgggagaatc gtcagagttc gcctgtgaca agagtcaaca ttgcgcagca ataaaatcgc
    16261 ctctccgtgt ggagaggcgc aggagattac gcgtccggga actcacagat aaagcgttcg
    16321 acatcttcaa ccatatggtc gttgccgaca aagaatgaac ggcgctggtg cagggtttcc
    16381 gggataatat ccagaatacg ctctttgcca tcgctcgctt taccgcccgc ttgttccgcc
    16441 aggaatgcca tcgggttgca ctcatacagc aaacgcagtt tgccgtccgg gtggctggcg
    16501 gtgcttgggt agagataaat accgcctttc agtaggttac ggtggaaatc cgcaaccagt
    16561 gagccgatat aacgtgaggt atacgggcgg ttggtggatt tatcttcttc ctggcagaat
    16621 ttaatgtact tcttcacccc gttcggaaac ttaatgtagt ttccttcgtt gatggagtag
    16681 gttttgcctt tctccgggaa gcgcatccgt tcctggcaca ggcagaaaac gccgagcgaa
    16741 ggatcgtaag taaaagcgtg aacaccgcat ccggtactgt aaaccagcat ggtagaggag
    16801 ccgtatacca cgtaacctgc cgcaacctgt ttgttaccag gctggaggaa atcttcttcc
    16861 gttaccggcg tgccgacggg cgtaactcgg cggtagatgg agaaaatggt accgacagag
    16921 acgttaacat cgatgttgga cgagccatcc agtgggtcca tcagcaccac gtattttgca
    16981 tgttcacagc cttcaaagac aacaatctca tcttcttctt cagaggcaat gcctgcaacg
    17041 atatcgcgtg ctttcagtgc ggctttcagt ttttcattag cgaacaagtc gagtttttgc
    17101 tgaacctcgc cctgcacgtt ctcagcaccg ctggcaccca ggatatcaac cagtcctgct
    17161 ttgttgatat cgcgatggat aatcttggcg cccagtttta ttgccgacag caaagcagtg
    17221 agctcaccgg tagcatgaga aaactcgtgc tgcttttcga caataaattc acctaacgtt
    17281 ttcataaaac tttccctgca atgtttatgg agtaaagcga ccgcaacaat cttaacaaat
    17341 aatctcaatg ttgcgctcag gtgaatcgcg ccagcaaatt acggattatc ctgaaatgcg
    17401 tttctcactt gcccgacata tgcgtaaaat gagcggcaga ttaaaaaagg atagtgacgc
    17461 atgcgcattc atattttagg aatttgtggc acgtttatgg gcggtctggc gatgctggcg
    17521 cgccagttag gccatgaagt aacgggttcg gacgccaatg tgtatccgcc gatgagcacc
    17581 ttacttgaga agcaaggcat tgagctgatt cagggttacg atgccagcca gctcgacccg
    17641 cagccggatc tggtgattat tggcaacgcc atgacccgtg gaaatccgtg tgtggaagcg
    17701 gtactggaaa aaaacatccc ttatatgtca ggtccacagt ggctgcacga ttttgtgctg
    17761 cgcgaccgct gggtgctggc cgttgccggt acacacggca aaaccaccac cgcgggaatg
    17821 gcgacttgga ttctggaaca gtgtggttac aaaccgggct ttgtaatcgg cggtgtgccg
    17881 gggaactttg aggtttcggc gcgtctgggc gaaagcgact tctttgttat cgaagcggat
    17941 gagtacgact gcgccttctt cgacaaacgc tctaaatttg tccattactg cccgcgtacg
    18001 ctgatcctca acaaccttga gttcgatcac gccgatatct ttgacgacct gaaagcgatc
    18061 cagaaacagt tccaccatct ggtgcgtatc gttccggggc agggtcgtat tatctggccg
    18121 gaaaatgaca tcaacctgaa acagaccatg gcgatgggct gctggagcga gcaggagctg
    18181 gtgggtgagc agggtcactg gcaggcgaaa aagctgacca ccgatgcttc cgaatgggaa
    18241 gttttgctgg atggcgaaaa agtgggcgaa gtgaaatggt cgctggtagg cgaacataat
    18301 atgcacaatg gcctgatggc gattgcagcg gctcgccatg ttggtgtagc gccggcagat
    18361 gccgctaacg cgctgggttc gtttattaac gctcgccgcc gtctggagtt gcgtggtgaa
    18421 gcgaatggcg tcacggtata tgacgatttt gcccatcatc cgacggcgat tctggcaacg
    18481 ctggcggcgc tgcgtggcaa agttggtggt acggcgcgca ttattgctgt gctggagccg
    18541 cgctcgaata ccatgaaaat ggggatctgc aaagacgatc tggcaccttc attaggtcgt
    18601 gccgatgaag tcttcctgct gcaaccggcg catattccgt ggcaggtggc agaagtggca
    18661 gaagcctgcg ttcagcctgc acactggagt ggcgatgtgg atacgctggc agatatggtg
    18721 gtgaaaaccg ctcagcctgg cgaccatatt ctggtgatga gcaacggcgg ttttggtggg
    18781 atccatcaga aactactgga tggtctggcg aagaaggcgg aagctgcgca gtaattcggc
    18841 ctcagcctga gatagtgtgc gtaggccgga taaggcgttc acgccgcatc cggcatttga
    18901 gcatagtgcc tgatgcgacg cttgatgcgt cttatcaggc ctacaggtgc accgcatccg
    18961 gcaaaccata ctcacatcaa caacgaaaat tacccttcat tctccgccag ttcgcgcaga
    19021 tactggaaaa tctggcgtgc ggatttcggc ggcttattcc cttctttctc tttcttcgcg
    19081 ttgcggatca gagtacgcag ttgctgacga tcggcatcgg gccacagatt taatacctca
    19141 gcgatggcgt catcaccttg atcgatcaga cgatcgcgca ggttttccag tttatggaac
    19201 agcaccacct gctggttgtg acggtttttc agcttatcca gcgcctgacg gatcggttct
    19261 acatcgcgct ggcgcagcat cttaccgatg agctgcaact ggcggcggcg accttccatc
    19321 ttaatacgct gggcgagctc aatagccgcg cgcagatccg catctaacgg gatcttatcc
    19381 agcgcgtttt tccccagatc aacaatttcc gcgccaaggc gttttagctc ctcggcatca
    19441 cgtttaattt cacttttact gacccagata atttcatcgt cttcgtcttc gatgtcatca
    19501 ccgggaacgt cgtcgagcca gtcttcgggc tgcttagtca tctcaggctc cttaaaaaaa
    19561 gaggctaatg ttaccagtta agatgcgcac tgaaaaacgg ttctctgtta gacttcagag
    19621 aaactctcta cattatggca cttgcaatga aagtaatctc tcaagttgaa gcgcagcgca
    19681 agattctgga agaagcagtt tccactgcgc tggagttggc ctcaggcaaa tcggacggtg
    19741 cggaagttgc cgtcagcaag accaccggca ttagcgtaag cacgcgttat ggtgaagtgg
    19801 agaatgttga attcaatagc gatggcgcgc tggggatcac tgtttatcac cagaaccgca
    19861 aaggtagcgc atcatccacc gatttaagcc cgcaggccat tgcccgtact gtacaggcgg
    19921 cgctggatat tgcccgttat acctcgccag atccctgtgc cggcgtggca gacaaagagc
    19981 tgctggcctt tgacgcaccg gatctcgact tgttccaccc tgcggaagtt tccccggatg
    20041 aagccattga actggcggcc cgcgcagaac aggcggcatt gcaggcggac aaacgcatca
    20101 ccaataccga aggtggcagc tttaacagcc actacggtgt caaagttttt ggcaacagcc
    20161 acggcatgtt gcagggttac tgctcaacgc gtcattcgct ctccagctgt gtaattgccg
    20221 aagaaaatgg cgatatggag cgtgattacg cctacaccat tggccgcgcg atgagcgatt
    20281 tgcagacgcc agagtgggtc ggggccgatt gcgctcgccg tactttatcg cgtctgtcac
    20341 cgcgtaaact ctccaccatg aaagcgccgg tcatttttgc caatgaagtg gcaaccgggc
    20401 tttttgggca tctggtgggg gcgatagcgg gtggatcggt ttaccgtaaa tctaccttcc
    20461 tgctggattc gctgggtaaa caaattctgc cggactggct gaccattgaa gagcatccgc
    20521 atctgctgaa agggctggca tcgacgccat ttgacagcga aggtgtgcgc accgagcgtc
    20581 gcgatattat taaagatggc atcctgactc agtggctgct gaccagctac tcggcgcgga
    20641 aactggggct gaaaagcacc ggacatgcgg gcggtattca caactggcgg attgccggac
    20701 aaggtctaag cttcgagcag atgctcaaag agatgggcac cgggctggtg gtgacggaat
    20761 tgatgggcca gggcgtgagt gcaattaccg gtgattattc ccgtggtgcg gcgggcttct
    20821 gggtagaaaa cggcgaaatt cagtatccgg ttagcgaaat caccatcgca ggtaatttaa
    20881 aagatatgtg gcgcaatatt gtcaccgtcg gtaacgatat tgaaacacgc agtaatatac
    20941 agtgtggttc tgtgctgttg ccggagatga aaatcgccgg acagtaaaaa aactggcgcg
    21001 acctgccgcg ctataaacta aaaaattaca caaaatcatt cgcactgcat cgagacggaa
    21061 agtgaatgag ggccggggag cgtactcgca gtacgtgacc cggtcgaatg agcgtagtcg
    21121 tcgatgaggc agtgtgaagg atgaagtgta aataaaaaag gaagtgagca atgcgaaaaa
    21181 gcctgttagc tattcttgca gtctcctcgt tggtattcag ttcggcgtcg tttgctgctg
    21241 atcttgaaga caatatggaa accctcaacg acaatttaaa agtggtcgaa aaagccgata
    21301 acgcggcgca agtcaaagac gcgttaacga agatgcgcgc cgcagcgctg gatgcgcaaa
    21361 aagcaacgcc gccgaagctc gaagataaat caccggacag cccggaaatg aaagatttcc
    21421 gccacggttt cgacattctg gtcggtcaga ttgacgacgc gctgaagctg gcaaatgaag
    21481 gtaaagtaaa agaagcgcag gctgctgcag agcaactgaa aacgacccgc aattcatatc
    21541 acaagaagta tcgttaattc ctcatttccc tgttgcctgc actcaggtaa cagggaactt
    21601 atcatcgcaa atgatgcacc acctgattgc tgctgccgcg ccagatcagg gaagggtctt
    21661 ttaaatcctg cacaaatttg ccgtcgacca gcacgttaat caaatcaaca acctgcattt
    21721 gcgcagcgtt gagttcgtcg agtttatagc ctgtccacac ccagatgtct ttacccggac
    21781 actcggcgcg gatgcgttgt accagtttca gaatatccgg cacgttttgc ggatgcagcg
    21841 gatcgccgcc ggagagggaa ataccctggc gtttgatacg agtgtcattc agatcgttaa
    21901 tgatctggtc ttccattgct ttagtaaatg gctgaccgga atttacccgc catgtgcttt
    21961 tgttatagca accggggcat tcatgaacac acccggagac aaacagggtg cagcgagtgc
    22021 cgggaccgtt gacgatgtcg acaggatagt actgatgata attcattatt tcgcctggct
    22081 tatcagtccg cgtgtaggct gtatcaggca atcaacgcct gatgcgacgc tgtcgcgtct
    22141 tatcaggcct acagccgttg ccaaatgtag gccggataag gcgtttacgc cgcatccggc
    22201 aatcaacgcg ggatgcgacg ctttcacatc acatccggca tttgcttagt gcagatcaac
    22261 ctatctgccc atttcccaaa tgtttaacgc ggcgcttaac ttcttcctgc ttgccggcgt
    22321 taaacggacg tgcatccggg ctacctaaat atccgcacac gcggcgagtt accgacacac
    22381 gggaggcgtc atggttacca catttcgggc aggtgaagcc tttgctggtg cactcgaact
    22441 caccggtaaa gccacactcg tagcactcat caatcggtgt attggtgccg taatacggta
    22501 catgctgata gctgtaatcc cagacatctt ccagcgcctt caggttgtgc tgaatgtttg
    22561 gatactcgcc gtagcaaatg aaaccaccgt tcgccagcgg cgggtaaggc gcttcaaagt
    22621 cgatcttgtc gtacgggttc accttcttct ccacatcgag gtggaaactg ttggtgtagt
    22681 aacctttatc ggtcacgccc ggcaccacgc caaactcagc agtatcgaga cggcagaagc
    22741 ggtcgcacag gttttcactc ggcgtgctgt agagactgaa accataaccc gtttcttctt
    22801 tccactgatc cactgcctga cgcagacgtt caacaatggc gatacctttc gcgcggagct
    22861 gctcgttgtc gtaaacatgt tcgccgccga acagcgcgtt aatggtttcg tggatgccga
    22921 tgtaacccag cgaaatagac gcacgaccgt ttttgaagat ttcagaaata tcatcgtcag
    22981 cattgagacg cacgccacaa gcaccttcca tatagaggat cggggccacg cgcgctttca
    23041 cgccttcgag acgagcgata cgggtcatca gagccttacg tgccagcacc agacgttcat
    23101 ccagcagctt ccagaaggtg gcttcatcgc cttttgcttc cagagcaata cgcggcaggt
    23161 tcaggctgat cacgccgagg ttgttacgac catcgtggat ctgctcgccg ttttcatttt
    23221 cccacacgcc gaggaagctg cggcagccca tcggggtttt aaacgaaccg gtgactttca
    23281 ctacctgatc gtagttcagg atatccggat acatgcgctt gcttgcgcac tccagcgcca
    23341 gctgtttgat gtcgtagttc ggatcgcctt ttttatggtt cagaccatcg cgaatcgcaa
    23401 acaccagttt cgggaacacc gcagttttac ggtttttacc gaggcctgcg atacggttac
    23461 gcaggataga ttcctgaatc aggcgcgact cccagctggt gcccagacca aaaccaaagg
    23521 ttacaaacgg cgtctgaccg ttggcggtgt gcagggtgtt tacttcgtac tccagcgact
    23581 ggaaggcatc gtagcactct ttaatggtac gagagttagc gtagccttcg gcgtccggga
    23641 tgttccactc ttctgcggtc ttgcgatgtt tgttgtagct ggcggtgaca aacggtgcca
    23701 gcacttcatc gatacggtta atggtggtgc cgccataaat atggctggca acctgagcaa
    23761 taatctgcgc agttaccgcg gttgccgtag agatcgactt cggcggttca atctcggcgt
    23821 tccccatctt aaagccctgg gtcagcatgc ctttcaggtc gatcaacatg cagttgaaca
    23881 tcgggaaaaa cggtgagtaa tcgagatcgt gatagtgaat atcgccacgc tcatgtgcct
    23941 gcaccacgtc acgcggcagc aggtgctgac gtgcatagtg tttagccacg atcccggcca
    24001 gcaggtcgcg ctgggttgga atcaccttgc tgtctttgtt ggcgttttcg ttgagtaacg
    24061 aggcgttggt ctgctcgacc agaccacgga tctcctgatt caggcgaccg cgtttttcac
    24121 gttcaatgtc gcgatcgtga cggtactcga tgtaagcacg agccagttgt ttgtatggac
    24181 ctgacatcag ctggttttca acagcggtct ggatctcatt gatatccacc tggttgcggc
    24241 cctgcatctg ctcgctgaca actgcagcaa cagtggcgca ataatcggca tcatcgactt
    24301 ccgctgcttt agctgcacgc agaatcgctt ctttgatgcg ctctgattta aacggcactt
    24361 tgcagccgtc tcgtttcatc acatgcggtg tcatgatcgc tccatatttt taagaacagg
    24421 ttatccacag aaattgggaa aggcgttttc cggttttttc gtttcgtttt ccggtgcttt
    24481 ccgcaatcgc catccgctta tccacaacgc cgcacctttt tcgtggggag ctgtagtagc
    24541 aattatagtc gattgataca acatattggg ttgggacgca ttttaaagtc tatatatagt
    24601 gctttgcatc aaggatgttt gaactttttt tgatgtagct caaagtaaaa agcagagcgt
    24661 acggatgacg ggtgctacag cgttatgtaa attttttaat gaatttgctg gttgaaaaat
    24721 caacaaaaac aacatgctga cagacaaaac cccggaatga ccggcattac cggggcttag
    24781 ggaagattta cttctgtaac caccagacag cctcaaaagg ccgtaaagtc atggcacagg
    24841 gttgtggtga ggcttcttcg tagttatgca tcacaagctg ccagttgccg cgcatttgcc
    24901 ctagctgcca gggttggatc tcacggctaa ggttggcaat gaccagcaag gtttgcccct
    24961 tccattcacg gcgatagcac cacaatacag ggctgtttgg cagcagatcc tggtaattgc
    25021 cccatgtcag gatggcttcc tgcttacgca gtgcgattaa cttttggtag gtgtaaaaca
    25081 ccgaggactc atcggccagc gcggcttcta cgttgatttg ttgatagtta tcgcccaggc
    25141 caatccacgg ttcgccagcc gtaaacccgg cattatcgcc gttgctccat tgcatgggcg
    25201 tgcggctgtt gtcacgggat ttactggcga ggattgccaa taactcgtcg gcatcacgcc
    25261 catcgttgcg cagctcggca aacatattga ggctctctac gtcgcgatag tcagtaatgc
    25321 gcgtgaaatg cgggttggtc atgccaatct cttcgccctg gtagatatac ggcgttccct
    25381 gcatgccatg cagcaccatc gccagcattt ttgccgcagg cacgcggtat tcaccttcat
    25441 caccaaagcg agaaacaatg cgcggctgat cgtggttaca ccagaacaag gcattcaatg
    25501 ctacgttgtg cattccttgt tgccagtggc ggaacaatgt tttcaacgcc acaaagtcag
    25561 gtttagccag cgtccatttt tcaccaccgg gataatcgac cttcaggtga tgaaaattaa
    25621 aggtcatcga caattcactg cctgtcagtg ccgcgtatcg ctggcaatgc tcaaggctgg
    25681 tggaggacat ttcacctacg gtcattaacc cgcgtggcgt aaacacatcg cggttcatct
    25741 cgtgcaaaaa ctcgtgtgct cgtggcccgt cggtgtagaa gcgacgcccg tcgccatcca
    25801 ggtcatcagg gaaacgcggg tctttggaga tcagattcac cacatccagg cgcaacccgt
    25861 cgaccccacg atcggcccag aactcacaga cttttttcag ctctgcgcgt accgctggat
    25921 tctcccagtt gagatccgcc tgttctggtg caaagagatg caaatagtac tgttcgcttt
    25981 ccgcatgcca gcgccacgca ctaccgccaa attttgaacg ccagttgttc ggtggcgttt
    26041 ctggttcgcc atcgcgccag atataaaact ggcggtaagg gctttctttg ttcagcgcct
    26101 cgcgaaacca ggcatgttgg gtagaggtat ggttaaacac catatcgaga atgatacgaa
    26161 tcccgcgcga ttttgcctgc gtcaccagtt cgtcaaaatc gtccagcgtg ccgtaggtgg
    26221 gatcaatcgc cgtatagttc gctacatcgt aaccgttatc gacctgcgga gagacataaa
    26281 agggggttag ccagatggca tcaacgccca gtttatgcag atagtccagg cgttggataa
    26341 cgccacgtaa atcgccggtg ccgctacccg tggtgtcctg aaaactcttt ggataaatct
    26401 ggtagataac gccgttttgc caccagtggg gaagattagt cattacgtta ttcctgcaaa
    26461 tgcgaggggg cgcaattgcg ccccgaagaa aattaaacaa tgtccagcgt gcccaggcgg
    26521 tatttccgct gatagataaa cgaggtgagt acaatcggga tgatgatggc gatagccatt
    26581 gccagcgcaa acacctgcca gtagctcggt tgaatcgaga gaattcccgg caggccgcct
    26641 acgccgatgc cattcgccat aacgccgttc aggccgcata gcaatcctgc cagaccagaa
    26701 ccaatcatcg cgcacagcat cgggaagcga tatttcaggt tgatgccgta cattgcaggc
    26761 tcagtgaccc caagccaggc ggagatcgcc gcaggcacgg agatctcgcg ttcattgtgc
    26821 ttgcggctgg aaatgataat gcctatcacg gcggagccct gagcgatatt cgacagcgca
    26881 atcagcggcc acactggcgt accacccatg ctttgaatca tctgcaaatc aatagcaagc
    26941 gtggtctggt gtacaccggt gatcaccagc ggggcgtaca ggaagccaaa caatgccgcg
    27001 ccaatcggag caaagctgcc ggtcatcagg tgacgtaccg caaaggcaac gccatcgcca
    27061 atcatgcgac caaacggacc aatcagcgca tgggcgagga acaccgcgag gatcagcgaa
    27121 cagacgggca ccaccaccag atagaggtaa tccggcacga tgcgtttaag gcgagtttca
    27181 ataacgccca gcgccagccc ggctaacagt gccgggatca cctgcgcctg atagcccact
    27241 ttggcgatgc tgaacatgcc aaagtcccac acttccggca gctgctgccc gagcagataa
    27301 gcgttcatca gttgggggga aaccagtgtc acgccaagca cgataccaag gatcggcgtg
    27361 ccgcccattt ttttcaccgc tgaccagcaa atcccgaccg gcaggtagaa gaagatcgct
    27421 tcaccgatca accacagaaa atcgtagatc gtttgcaggg aagggtacat ttgcgccagc
    27481 gtttgaccgt tgctcatggg caaatcgccg atcacattgc gaaaaccgag gatcaaaccg
    27541 ccgctaatca acgcgggcag caacgggaag aagatctctg cgaaatgaga gatcaactgc
    27601 tcatgccatt tcatattctg ccgggcggct ttttttacct gctctttatc aacctgcgcc
    27661 tgtccggttg acgctatcag tgcttgatag taatcaccca cgttggtgcc aatcaccacc
    27721 tgaaattgcc cggcattggt gaaacagcct ttcaccatgg ggagttgctc aatttctttc
    27781 ggtctggcat tggccggttg gttgaggaca aagcgcaggc gagtaataca gtggctcacc
    27841 gtcgcaatat tgccgcgccc gccgaccagt tcaatcaacc gatcgatatc cgtttggttt
    27901 attttgctca tcataaagcc ccatggcaga tgacattttt ggttggctgc agaatatatt
    27961 gcgcggaaaa atttaaaaac gggaacgttc ccgaaacgca gcgaagatca caatttatcg
    28021 ttcaggaaac gatcaggaca ggttggcggg gatgatgatt tgttgcggtt cgctgcgtcc
    28081 ggttacctgc gcgatcaact ggcaagccgc ctggcgtcca gcttcggcgt aaccaggatc
    28141 tacggtgacg atctccggat gaaggaattt cattaacggc gtattaccga cgctcgccag
    28201 ttgcaaggtg tcgatgcgtt gctcttgcag gtatttactt gcgccaagtg ccagcgtgtc
    28261 ggttgcgcac agtaaggcgg tagtttcagg cgtaatcact tttgctacgt tctcatagcc
    28321 ttgcttcata gcaagccctg gcagggcggc aacgggatgc agtttatgcg ctttgcagaa
    28381 cgccaggtag gcttcgtgac gtcgcttacc ggttgtcacg tcactgtgcg gcacgccgag
    28441 ataactgata ttacgatgcc cctggtcata cagccgttgc atcaggatct tgattgcccc
    28501 ttcgtcgtca taacagaccg aagcaaagcc ttttgcgtca cgcgccagca gaaccagcga
    28561 tgactgccag tgggctaaca tttcttctgt tatgccagta aaaccgaaca gcactacgcc
    28621 gtcgatatta cgccgtttca gcacccccaa atgttcggca actaattgcg gggaaaactg
    28681 actttccatc atgattgggt cgtaaccttg ttcatagaac gctggcagca tggtttgaac
    28741 ggcgagattt tctgacaacg aatccagacg ggtaacaatg atggcgacca ctttatcgct
    28801 ttgcccacgc atagcgcgcg cagaacggga aggggaaaat ccatgctgat tcatcactgc
    28861 ttcaacacgc tcgcgggtgc gctggctcac gccgctttcg ttattcagca cccgggaaac
    28921 tgtagatttc cccacgccgc ttaagcgcgc gatgtctttg atggtcagcc gattttgcat
    28981 cctgttgtcc tgtaacgtgt tgtttaatta tttgagccta actttacccg tgcattcagc
    29041 aatgggtaaa gtctggttta tcgttggttt agttgccagc aggtattata tcgccataga
    29101 tgctacgaat attattggat tctccttatt atttgcggcg cttttttcac ttaccggagg
    29161 ttatatggaa cctgatccca cgcctctccc tcgacggaga ttaaaacttt tccggtaagc
    29221 ccgtcttttc acggcgttac cggatgcgta aggccgtgac gttttaacgt ccctgctcag
    29281 ctttattacc ttcaggtaag gcttcgccac gcctgaagac atttctgtac tgtttcagac
    29341 agtgcggagg gactccttat gtttaaagaa atttttaccc ggctcattcg ccatttacct
    29401 tcccgtctgg ttcatcgtga tccattgcct ggcgcgcagc agacagtgaa tacggtggtc
    29461 ccgccgtcct taagtgcgca ttgcctgaaa atggcggtga tgcccgaaga agaattgtgg
    29521 aaaacgttcg acacccatcc ggaagggtta aatcaggcgg aagtggaatc tgcccgcgaa
    29581 caacatggtg aaaataaatt acccgcacaa caaccgtcgc cgtggtgggt acatttatgg
    29641 gtctgctatc gcaacccctt taatatttta ctcaccattc tcggcgctat ttcttacgcc
    29701 acggaagatt tatttgccgc gggcgttatc gcgctaatgg tcgctatttc aacgttgctg
    29761 aactttattc aggaagcacg ttccactaaa gcggcagatg ccctgaaagc gatggtcagc
    29821 aataccgcga cggtgctgcg cgtaattaac gacaaaggcg aaaatggctg gctggagatc
    29881 ccgatcgacc agctggtgcc cggcgatatt ataaaactgg cggcgggaga tatgatcccg
    29941 gcagatttac gtatcttgca ggcgcgggat ctgttcgtcg ctcaggcgtc gttaaccggt
    30001 gagtctctgc ccgtggaaaa agccgctacc actcgccagc cggagcacag caatccgctg
    30061 gagtgcgaca cgctgtgttt tatgggcacc accgtggtga gcggcacggc acaagcaatg
    30121 gtgattgcta caggtgccaa tacctggttt ggtcaactgg cggggcgtgt tagtgagcag
    30181 gaaagcgagc cgaatgcctt tcagcaaggg atcagccgcg tcagtatgct gctgattcgc
    30241 tttatgctgg tgatggcacc ggtggtgctg ttaatcaatg gctacaccaa aggcgactgg
    30301 tgggaagcgg cgctgtttgc gctttcggtc gcggtaggcc taacgccgga aatgttgccg
    30361 atgattgtta cctcgacgct ggcgcgcggc gcagtaaagc tgtcgaaaca gaaagtgatc
    30421 gtcaaacatc tggatgctat tcagaacttt ggcgcaatgg atattctgtg cactgataaa
    30481 accggcaccc tgacacagga taaaattgtg ctggagaatc ataccgatat ctccggtaaa
    30541 accagcgaac gcgtgctgca tagcgcgtgg ttgaatagtc attatcagac cggacttaaa
    30601 aacctgctcg atacagcggt gctcgaaggt acggatgaag agtcagcgcg ctcgctggcc
    30661 agtcgttggc aaaaaatcga tgagattccg tttgatttcg agcgtcgccg gatgtcggtg
    30721 gtagtggcag aaaataccga gcaccatcag ctggtttgca aaggtgcatt gcaggaaatc
    30781 ctcaatgtgt gttcgcaggt gcgtcacaat ggcgagattg tgccgctcga tgacatcatg
    30841 ctgcgtaaga ttaagcgggt tactgatacg ctgaatcgtc aggggctgcg cgtggttgcg
    30901 gtggcgacga aatacctgcc agcgcgtgaa ggagattacc agcgggcgga tgaatccgac
    30961 ctgatcctcg aaggatatat tgctttcctt gatccgccaa aagagacaac cgctccggca
    31021 ctgaaggcat taaaagcgag tggaattacc gtaaaaatcc ttactggcga cagtgagtta
    31081 gttgcagcga aagtgtgcca tgaagtgggg ctggacgcgg gagaggtggt cattggtagt
    31141 gatattgaaa cgctatctga cgacgaactg gcaaatcttg cacagcgcac tacgctgttt
    31201 gcccgcctga cgccgatgca taaagaacgc attgtgacct tactgaagcg cgaagggcat
    31261 gtggttggct ttatgggcga tggtattaat gatgcgcccg ccttacgcgc tgcggatatc
    31321 ggcatttctg tggacggcgc ggtagatatt gcccgtgaag cggctgatat catcctgctg
    31381 gaaaaaagcc tgatggtgct ggaagagggg gttattgagg gacgtcgcac tttcgccaac
    31441 atgctgaaat acatcaaaat gacggcgagt tctaacttcg gtaatgtgtt cagcgtgctg
    31501 gtggcgagtg ctttcttgcc cttcctgccg atgttgccgt tacacttgct tattcagaac
    31561 ctgctgtacg atgtgtcaca ggtggcgatc ccgtttgata acgtcgacga cgagcaaatt
    31621 caaaagccgc agcgttggaa tccggcggat ctggggcgct ttatgatctt cttcggaccg
    31681 atcagctcga tcttcgatat tctgacgttt tgcctgatgt ggtgggtatt ccatgccaac
    31741 acgccggaaa cgcaaacgct gttccagtcg ggatggtttg tggtgggctt actgtcgcaa
    31801 acgctgattg tgcatatgat ccgcacccgc cgtgtgccgt ttattcagag ctgtgcatcg
    31861 tggccgttaa tgatcatgac cgtgatcgtg atgattgtcg ggatcgcatt gccgttttca
    31921 ccgctggcca gttatctgca attacaggcg ctgccgttaa gctatttccc gtggctggtt
    31981 gcgattctgg cagggtatat gacattaacc cagttggtga aagggttcta tagccgtcgt
    32041 tacggctggc aataaagaat aaactgggca cgatagcccc gattcagatt gcagacaaag
    32101 taagctttgt tcatgccgga tgcggcgtaa atgccttatc cggccaacaa attcaataaa
    32161 ttgcaggaat cacgtaggcc tgataagcgt agcgcatcag gcgattttgc gtttgtcatc
    32221 agacttaatc cgggcatgat agcccggatt tccatcaaga ttagcgacga acagcgatcg
    32281 cttcgatctc aatcttcacg tctttcggca gacgggcaac ttcaacgcaa gaacgtgccg
    32341 ggaaggtggc gttgtgttcg gtgaagaagg cttcgtaagt ggcgtttacg gttgcgaagt
    32401 cgttcagatc ttttacaaac acggtagttt taacgatgtc gcccactttc agaccagcgg
    32461 cttcgacgat cgcttttacg ttatccagcg actgacgtgc ctgtgcagcg acgtctgccg
    32521 gtacttcgcc cgttttcgga tttaccggga tctgaccgga agtgatgatc atattgccca
    32581 gatcaacgcc ctgtacgtaa ggaccgatag ctgccggtgc attttccgtc gcgatagttt
    32641 tgctcatgat ttctccttta ttacagcggt aaaaagtctg gctcattata gggagccaga
    32701 cttttattac caaccgcaat taattggcca gcaccacatt atgggaaaac tctttttcac
    32761 agtatttgca tttgagcgcg atatcattgg cgcgttttcg cacggcaaag ctggaagaaa
    32821 ccggttcggc atggctgata cagttgctgt tcgggcagac cagcacattg tcgatgcgct
    32881 ccggcagact tgggcgcgat ttacccacca cttcatagtt gtcgatacgg ttaaccgtgg
    32941 cttgcggcgc atacaatgcc agttgatcta cctggtcttc actcaaaaag gtattttcga
    33001 ttttgatcag atctttgcgg cccatctcgc cagaaggcag gttcagaccg atggtgatgc
    33061 gctgatccgt ttcggtcagc ttgaacagac tcaacagctt aaaaccgatc tgggcgggga
    33121 tatggtcaat taccgtgccg cgtttaatag cttcaacctg caatttatta tcgtgtgtca
    33181 tctctatttc cccttacagt accagatcgc gattcagaac cagtgccagt aacgcctggc
    33241 gagcgaaaat cccgttgcct gcctgctgga agtaccaggc gtgtggcgtt ttatcaacat
    33301 ccgtcgcaat ctcatcaaca cgcggcagcg gatgaagtac tttcatattg gctttggcgt
    33361 tgtggagatc gctggcgcga agaacaaact gcgctttcac gttggcgtac tcggacgggt
    33421 ccagacgctc tttttgcacg cgggtcatgt acaggatgtc aacttccgcc atcacttctt
    33481 caatagagct gtgcagactc catgcgatcc ctttttcatc aagcatatcc agaatgtatt
    33541 gcggcattgc cagcgcgtcc ggcgcgatga agtaaaaacg gttgccgtcg aacttcgcta
    33601 acgcctgagt cagggagtga acggtgcggc catatttcag gtcaccaacc attgcgacgt
    33661 ggagattgtc cagacgcccc tgggtttcct gaatggtgaa taagtccagc aaggtttgcg
    33721 tcggatgttg gttggagcca tcaccggcat tcagtaccgg tacattgccg gaaaactcgg
    33781 tggccaggcg cgccgcacct tcctgcggat gacgcatcac tatcgcatcg acgtaagtgc
    33841 tgataaccga aatggtatcg gccagcgttt cgcccttttt acccagtgat gtattggcgc
    33901 tgtcggagaa gcccaccacg ctggcaccca ggcggtgcat ggaagtttcg aaagagaggc
    33961 gggtacgggt agaggcttcg aagaaacagc tggcaatgac tttgtgcttc aacagctctg
    34021 gttgcgggtt tgctttcagt ttcgccgctg tcgccagcac cagattaagg tcatcgcgac
    34081 taaggtcgtt tatggaaatg atatgtttct gatatagcgg attagccatc ttttatctcc
    34141 tgacgcctgg gcaaaaaaaa gcccctcgat tgaggggctg ggaatgggtg atcaacggga
    34201 agaaaaacgg caggccagcg tcttttttca gacgcggtaa gacaaaatgt cgaacacact
    34261 gaaccataca tcctcccggc aaattgtccg gcattatact catcgtcaga agcggtgcaa
    34321 gcatttgatg caatattttg tcagcgcaaa cggtttattt gaattataag tcaaaatata
    34381 tgcattttta tttactgtga ttctggaggg gaactgtcgt tcggtatcaa taattgcaga
    34441 catttcctgc ttttttcttt ttcactttca cgcaatcaga tatgcatttt attccattca
    34501 tccgacttat agggcggagt gtgaaaacga acggctaaca ctattgctta cagctcaggg
    34561 atgcgcgcta tcactttaat ttcaaaatca aagcctgcca gccatgtaac gcccaccgcc
    34621 gtccaggttg gataaggtgg cgcgctaaat atttcatttt tcaccgtcat gatgtcttca
    34681 aattgttttt ctggatcggt atgaaagctc gtaacatcaa tgatatcgtc aaaagtgcat
    34741 cccgcagctg ccagggtcgc atgcaaatta tcaaatgcca gtctgacttg ttgctgaaaa
    34801 tcgggttctg gtgttccgtc ctctcgactt cctacttgcc cggaaacaaa cagcaaatcg
    34861 ccggaacgaa tagccgcaga ataacgatgt tctgcataca gtgaatgtcg gccagcaggg
    34921 aaaacagcgg ttctttctac catttggtta tcctcaagat ttacgacatg aacagaagat
    34981 ttatatttac caggagccgc ttttagcgga cgacgtgagt aaacaaaacc cagacatcat
    35041 gggaaatggc tgggcttaat tgagcgtagt cggttatgcg ccaaacgcgc catcaatggt
    35101 atgcatcgcg ccggtaacaa aactggcttc tggccctgct aaccatgcga ccataccagc
    35161 gacctcttcc ggttgcccat gtcttttgat agccataaaa ctatgcaaca tatcgcgcat
    35221 tggcccgttg gcgggattag cgtcggtatc aattggccct ggctggacga cgttgatggt
    35281 gatcccacgc ggtccaaaat cacgggccag cccgcgcgcc atgccttgta gggcagattt
    35341 actggcggca taagcagcca tgcctgcaac aggcatacga tcgccattca cggagccgat
    35401 gattaagatg cgcccacctt cgggcatctg ccgggcggct tcaacagagg catggtaagg
    35461 agcatgaata ttgattttga aaaggcgatc aatatcgtcg gcatttaatt ccagggcatc
    35521 gccaaagacg ccaatacctg catttaccac caggatatcc aatgcgccgc tcttacgaac
    35581 gacatcaatg acagcgtctc tgtcagcact atctgtaaat actgctgtcg ctcccgtctc
    35641 ttgtgccagg cgttcagcgg catctttcga ccccgcatag gtgaatcgta cattggcccc
    35701 atcggtgacg aaacgacgta cgatagcggc accgattcca cgactgccac cgaggatgag
    35761 aactgtctta cctgtaaaag cgcccataag gactccttga tttattatgt aacatgcatt
    35821 acaaaactgt tttaactttc tgtcaacaag ttttgtaatg gttactaaaa aacaatctcg
    35881 cgttccaggt cgtcccagac gtttcgctcc tgagcaggca gtctctgcgg caaaagtgct
    35941 ttttcaccaa aaaggtttcg atgctgtcag tgttgctgaa gttactgatt atcttggtat
    36001 taaccccccg agcctctacg cggcttttgg cagtaaggct gggttattta gccgtgtact
    36061 caatgaatat gtcggtacgg aagctattcc gcttgtcgat attcttcgtg atgatcgtcc
    36121 ggtaggggag tgcctggctg aggtattaaa agaagcggcg cgcagatata gccaaaacgg
    36181 cggctgcgct ggctgtatgg ttcttgaagg cattcatagt catgatccac aagcgcgtga
    36241 tattgccgtt caatattatc acgccgcaga aacggccatt tatgactata tcgccaggct
    36301 gcatccacaa agcgcacaat gtgtgactga ttttatgagt accgtgatgt cagggctttc
    36361 tgcgaaggca cgagaggggc actccctcga acaactctgt gcaactgctg cactggcggg
    36421 ggaagcgata aaaactattc tcaaggagtg atgctggcct tgatccgaaa ggcgggaacg
    36481 cgcctgccga taagttgtga taagacaata attcacgcgt taaggctggc tgaattgatc
    36541 atcttttcgt ataacggtgg aaatgaaaca ttgttttaat taaggagtgg aaaagatgat
    36601 catcggaaat attcataatc ttcagccgtg gctaccccag gagttacgcc aggcgattga
    36661 gcatatcaaa gcacacgtta cggcagaaac gccaaagggc aagcacgata tcgaaggtaa
    36721 tcgtctgttt tatcttatct cggaagatat gaccgagccg tacgaagctc gccgtgcgga
    36781 gtaccatgcc cgctatctcg acattcagat tgtgttaaaa ggtcaggaag gcatgacctt
    36841 cagcacacaa ccggcaggta cgccggatac cgactggtta gctgataaag acatcgcatt
    36901 tttgccggaa ggcgttgatg agaaaacagt tatccttaat gaaggtgatt ttgttgtgtt
    36961 ttatccgggg gaagtgcata aaccgctgtg cgcagtgggt gcaccagccc aggttcgcaa
    37021 agcagtagtg aagatgctga tggcgtgatg acttttcgcc gtaaataact ccaggtttac
    37081 ggcgagtttg tgaaaagagc gttttttgat atttttttgt gagtaaaatt tgtaatgctt
    37141 agacgttctt attaactcaa ggagttcgtc atgagcaaaa tatcagattt gaattattct
    37201 caacacatta cattagccga caattttaaa caaaaaagtg aagttttaaa tacctggcgt
    37261 gttggaatga ataattttgc ccgtaatgcc ggggggcagg ataacacaag aaatatcctt
    37321 aatcctaaga catttttg
//
LOCUS       DBFIFM010000019        36864 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.019, whole genome shotgun sequence.
ACCESSION   DBFIFM010000019 DBFIFM010000000
VERSION     DBFIFM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.019"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            93..1676
                     /gene="yegH"
                     /locus_tag="N7C74_000903"
     CDS             93..1676
                     /gene="yegH"
                     /locus_tag="N7C74_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HGU2430828.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            1751..2089
                     /locus_tag="N7C74_000904"
     CDS             1751..2089
                     /locus_tag="N7C74_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU2430829.1"
                     /translation="MRKKLAFLDTSLDDLRAFPESSRQEIGYQLDRIQQGLNPYDWKP
                     FSTIGPGVREIRTRDADGIYRVMYVAKFEEAVYVLHCFQKKTQTTSQSDIDLAKRRYK
                     ELVQERKNEN"
     gene            2079..2369
                     /locus_tag="N7C74_000905"
     CDS             2079..2369
                     /locus_tag="N7C74_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2430830.1"
                     /translation="MKIETFDSVWDAVSDTPEQAENMRIRAELVTIINNWIEQQGFSQ
                     AQAASALGVTQPRISELARGKIQIFSIDKLITMMAHAGLHIQRIEVQYPHAA"
     gene            complement(2422..4275)
                     /gene="asmA"
                     /locus_tag="N7C74_000906"
     CDS             complement(2422..4275)
                     /gene="asmA"
                     /locus_tag="N7C74_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HGU2430831.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESRKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMADTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLAAQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4297..4878)
                     /gene="dcd"
                     /locus_tag="N7C74_000907"
     CDS             complement(4297..4878)
                     /gene="dcd"
                     /locus_tag="N7C74_000907"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HGU2430832.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4970..5611)
                     /gene="udk"
                     /locus_tag="N7C74_000908"
     CDS             complement(4970..5611)
                     /gene="udk"
                     /locus_tag="N7C74_000908"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HGU2430833.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5929..9247
                     /gene="dgcE"
                     /locus_tag="N7C74_000909"
                     /pseudo
     CDS             5929..9247
                     /gene="dgcE"
                     /locus_tag="N7C74_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(9348..10196)
                     /gene="alkA"
                     /locus_tag="N7C74_000910"
     CDS             complement(9348..10196)
                     /gene="alkA"
                     /locus_tag="N7C74_000910"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HGU2430834.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSADLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            10330..11682
                     /gene="yegD"
                     /locus_tag="N7C74_000911"
     CDS             10330..11682
                     /gene="yegD"
                     /locus_tag="N7C74_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU2430835.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARIQEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11695..13635)
                     /gene="yegI"
                     /locus_tag="N7C74_000912"
     CDS             complement(11695..13635)
                     /gene="yegI"
                     /locus_tag="N7C74_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HGU2430836.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLPSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATARPTAKAWVAALDLLRQQLKKCTVSAMHVYPGHLTDCPWCALDNQGVIY
                     FIDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKVYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDAASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPAERQAVMAKMAAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13632..14393)
                     /gene="pphC"
                     /locus_tag="N7C74_000913"
     CDS             complement(13632..14393)
                     /gene="pphC"
                     /locus_tag="N7C74_000913"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HGU2430837.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAINEAMAYVSQKVQHGEFGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDVVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLAAATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLTLALWLP"
     gene            complement(14390..15049)
                     /gene="yegL"
                     /locus_tag="N7C74_000914"
     CDS             complement(14390..15049)
                     /gene="yegL"
                     /locus_tag="N7C74_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HGU2430838.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADIKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(15270..15329)
                     /gene="ibsA"
                     /locus_tag="N7C74_000915"
     CDS             complement(15270..15329)
                     /gene="ibsA"
                     /locus_tag="N7C74_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HGU2430839.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15601..15657)
                     /locus_tag="N7C74_000916"
     CDS             complement(15601..15657)
                     /locus_tag="N7C74_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU2430840.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            complement(15929..15985)
                     /locus_tag="N7C74_000917"
     CDS             complement(15929..15985)
                     /locus_tag="N7C74_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU2430841.1"
                     /translation="MMKILIIVVLLVISYPAY"
     gene            16265..17512
                     /gene="mdtA"
                     /locus_tag="N7C74_000918"
     CDS             16265..17512
                     /gene="mdtA"
                     /locus_tag="N7C74_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HGU2430842.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLTNARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            17512..20634
                     /gene="mdtB"
                     /locus_tag="N7C74_000919"
     CDS             17512..20634
                     /gene="mdtB"
                     /locus_tag="N7C74_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HGU2430843.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAITLVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQISDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20635..23712
                     /gene="mdtC"
                     /locus_tag="N7C74_000920"
     CDS             20635..23712
                     /gene="mdtC"
                     /locus_tag="N7C74_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HGU2430844.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGMVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKSKQTVTE"
     gene            23713..25128
                     /gene="mdtD"
                     /locus_tag="N7C74_000921"
     CDS             23713..25128
                     /gene="mdtD"
                     /locus_tag="N7C74_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2430845.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAITGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSSTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            25125..26528
                     /gene="baeS"
                     /locus_tag="N7C74_000922"
     CDS             25125..26528
                     /gene="baeS"
                     /locus_tag="N7C74_000922"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HGU2430846.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26525..27247
                     /gene="baeR"
                     /locus_tag="N7C74_000923"
     CDS             26525..27247
                     /gene="baeR"
                     /locus_tag="N7C74_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HGU2430847.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLSYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            27438..27770
                     /gene="yegP"
                     /locus_tag="N7C74_000924"
     CDS             27438..27770
                     /gene="yegP"
                     /locus_tag="N7C74_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HGU2430848.1"
                     /translation="MAGWFELSKSSDSQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27918..29279
                     /gene="yegQ"
                     /locus_tag="N7C74_000925"
     CDS             27918..29279
                     /gene="yegQ"
                     /locus_tag="N7C74_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HGU2430849.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29553..29771)
                     /gene="ogrK"
                     /locus_tag="N7C74_000926"
     CDS             complement(29553..29771)
                     /gene="ogrK"
                     /locus_tag="N7C74_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HGU2430850.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(29853..31016)
                     /locus_tag="N7C74_000927"
     CDS             complement(29853..31016)
                     /locus_tag="N7C74_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HGU2430851.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPIRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREDSWHDTTLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQVTITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVRQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSIILATGRADIYT
                     ETPVRVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            complement(31016..31495)
                     /locus_tag="N7C74_000928"
     CDS             complement(31016..31495)
                     /locus_tag="N7C74_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2430852.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENMTLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISRVSETG
                     SIFFADGTPRKIDFTLSLTRVDEPLAALYGDIGKQAESLIGKAGSMATKFTGMTGAG"
     gene            complement(31510..33957)
                     /locus_tag="N7C74_000929"
     CDS             complement(31510..33957)
                     /locus_tag="N7C74_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU2430853.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRETQKSLRE
                     LNGQASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDELGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTMAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSAEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPIFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAAFKASDGKTAELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRNLTQTATKYVLKLDGWIQKNKSLASTIGLIVGGALALIGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPLFDWLGEKLQAAWQWFKKLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(33950..34105)
                     /locus_tag="N7C74_000930"
     CDS             complement(33950..34105)
                     /locus_tag="N7C74_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HGU2430854.1"
                     /translation="MTFPKNLSVDDLMADVAVVFHWPPSELYPMSLTELITWREKALR
                     RSGNTNE"
     gene            complement(34102..34377)
                     /locus_tag="N7C74_000931"
     CDS             complement(34102..34377)
                     /locus_tag="N7C74_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HGU2430855.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPNAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(34434..34952)
                     /locus_tag="N7C74_000932"
     CDS             complement(34434..34952)
                     /locus_tag="N7C74_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HGU2430856.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(34965..36155)
                     /locus_tag="N7C74_000933"
     CDS             complement(34965..36155)
                     /locus_tag="N7C74_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HGU2430857.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVSSTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVNGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESSDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
     gene            complement(36215..36808)
                     /locus_tag="N7C74_000934"
     CDS             complement(36215..36808)
                     /locus_tag="N7C74_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU2430858.1"
                     /translation="MLIGYVRVSTNDQNTELQRNALECAGCELIFEDKISGTKSDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVILVEELRERGVNFRSLTDAIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLEAARAKGRIGGRRPKLTTSEWEQVGRLLAAGES
                     RQRVALIFDIGLSTLYKKFPSSATKNKLCHPLANRDK"
BASE COUNT         8295 a        10031 c         9719 g         8819 t
ORIGIN      
        1 ataaatagtg ctgcaacatt gcatttttgc cccgatttat ccatgatcga attgtgacat
       61 ttgtcataca acgaataggt tttgtactta ctatggaatg gattgccgat ccgtctatct
      121 gggccgggtt aatcacgctg attgtgatcg aactggtcct cggcattgat aacctggtct
      181 ttattgccat cctcgccgaa aaactaccgc cgaagcagcg cgaccgcgca cgggttaccg
      241 ggctgttgct ggcgatgtta atgcgcctgt tactgctggc gtcaatctcc tggctggtca
      301 ccctgactca accgctgttc agcttccgct cgtttacctt tagcgcccgc gacttaatca
      361 tgctgtttgg tggtttcttc ctgttgttca aagccacgat ggagctgaac gaacggctgg
      421 aagggaaaga cagcaataat cccacacaac gcaaaggtgc gaagttctgg ggggtggtga
      481 cacaaattgt ggtactggac gccatcttct cactcgactc agtgattacc gccgtcggga
      541 tggtcgacca tttactggtc atgatggccg ccgtggttat cgctatcagc ctgatgttga
      601 tggccagcaa gccgttaacg caatttgtta acagtcaccc gaccatcgtt attctctgct
      661 taagcttcct gttgatgatt ggctttagcc tggtggcaga aggtttcggc ttcgtcattc
      721 cgaaaggcta cctgtacgct gccattggtt tctcggtgat gatcgaggcg ctcaatcagt
      781 tggctatctt taaccggcga cgttttcttt ccgctaacca gacactgcgc cagcggacta
      841 ccgaagcggt aatgcgcctg cttagcgggc aaaaagagga tgcggaactg gacgccgaaa
      901 ccgcgtccat gctggtggat catggtaacc agcagatctt taatccgcag gaacggcgga
      961 tgattgagcg ggtacttaat cttaaccagc gtaccgtcag cagcattatg acgtcgcgcc
     1021 acgatattga gcatatcgat ctcaacgcgc cggaagacga gatccgccag ttgctggagc
     1081 gaaatcagca tacgcggctg gtggttactg atggcgatga cgcggaagat ttgctcggtg
     1141 ttgttcacgt tatcgacctg ttacaacaat cactgcgcgg cgaaccgctc aacctgcggg
     1201 tgttaattcg ccagccgctg gtgttcccgg aaaccttgcc gttgttacct gccctggagc
     1261 agttccgtaa tgcccgcaca cactttgctt ttgtggtgga tgagtttggc tcggtggaag
     1321 ggattgtgac attaagtgac gtcactgaaa ccattgccgg taacttaccg aacgaagtgg
     1381 aagagatcga cgcccgccat gatattcaga agaatgcgga cggttcctgg acggcgaatg
     1441 gtcatatgcc gctggaagat ctggtgcaat atgtgccgct gccgctggat gaaaaacgtg
     1501 aatatcacac cattgccggg ctattgatgg aatatttgca gcgtattcca aaacctggcg
     1561 aagaagttca ggtgggggat tatttgctta aaacgttgca ggtggaaagc catcgcgtgc
     1621 agaaggtgca gattataccg ctgcgtaagg atggcgagat ggagtacgag gtgtgataaa
     1681 acatcaaatg cgcaggttca aactgcgcat ttgatttata caaaatttag tataatctaa
     1741 ggatggagta atgagaaaaa agctggcttt tcttgatacc agtcttgacg atttacgcgc
     1801 atttccagaa tcgtctcgac aggaaattgg ctatcaactc gacaggatcc aacaagggtt
     1861 aaacccatac gactggaaac ctttttcaac tatcggccct ggtgtgcgag agattcgtac
     1921 tcgcgatgct gatgggatct atcgcgtcat gtatgtcgca aaatttgaag aagcggttta
     1981 cgtattgcat tgttttcaaa agaaaacaca gactaccagt caatcggata ttgatttagc
     2041 caaacgacgc tataaagaac ttgttcagga gcggaaaaat gaaaattgaa acctttgata
     2101 gtgtgtggga tgcggtgagt gacacccctg aacaagcgga aaatatgcgg atccgtgccg
     2161 agcttgtaac gatcattaat aactggattg aacaacaagg gtttagccag gctcaggcag
     2221 cttctgcttt aggggtcact caacctcgca tctctgaact tgccagaggt aaaatccaga
     2281 tctttagtat cgataaattg atcaccatga tggctcatgc aggactacat attcaacgta
     2341 ttgaagttca atatccacat gctgcttagt gctataaata cagatggcga caaattgatg
     2401 ccgccatcta tataacttgt cttacatctt ctccagcaac ttcttcacat ctttgccatt
     2461 gcgggaatct ttattccgct cggcccagtc attcaggcga cgtttcgctt cgtcctgtag
     2521 atgtttgcgc agtaactgat ccacttgcag actgtaattg agttgctgcc agttgccata
     2581 aacccgcagc ggtactggcg tttctttgag gaaatcaatc agtttgcttt ccccgttcca
     2641 gccacccacg acccgaatat caaactgggt gtcgcaggtt tgagctgcca gattcaacat
     2701 gccttcccct gtaagcgcca gcacaggcga ttgaccttgc atgtcgttta acgtcacgac
     2761 gccatccttc aacgtcaaat cggtggtaaa gcggtcaaga cgcgttacgt tatcgaagtt
     2821 ttcagcggcc ttcacatcac caccattacg ttctaccgct tgctgaatca tctgctggaa
     2881 gttcatccct tccatgcgcg tatcggccat ttcgacatgt gcctgtcctt gccagttgtg
     2941 gcggaatgcg tcggcatcta tgtcagcacc ggagaaatca ccagccaatg acatttttcc
     3001 ggtcaacgaa atcggatagt taaacgcctt cagaatggtg ccaatctcaa cgttttccag
     3061 ccgtggctgg aagtttatcc gcggatttat tgatgtcgcg tccagcgtgc ccggcagtga
     3121 aacctgtcca ccgttaagct tgccctgtag ttgagtaatt tccagcaaac ccgacttgtt
     3181 ggtcatttgc gtggcaacat ctgtaaaatt cattccgcgc cagcgcacgt tactggcctg
     3241 caacaaaata tcagccgtaa agccttgcag tccctgatag gccggttcat caatacgcga
     3301 agaaataacc gggcgcggca acgttgattg gctctgcccc tgctgcgcgg caccgttttc
     3361 accattcgcg gtttcattaa gcgggatgag gttgtcaaga ttcagttgcg ggaattgcag
     3421 cctcagctgc cattccggtt tctctgtcag cgtgacctgt gcttgcccgc tcagcgtact
     3481 atcattggcg gtcaaactaa tttggttaaa tgaaaggcgt ttacgtgact cctgccactg
     3541 ggcctggaaa ctcccctgcc cctgaatacc ttgttttggt aaatcggcac cctgcaactg
     3601 ccagttaatt tgttcaatag ccgccgttaa atcatgcgga taatcagaag catctaccgt
     3661 accgttaagg gatatcgtga gatcgcgctg atcgcgatta acccgcccgg agaactcaaa
     3721 tgagccacga tgttggggat cttgttccat ttgcaggcgg atattgcgga ttgtcacctg
     3781 ctcgtcatct tcatgctgga acaccagcac gctgtccgcc accttaagac tggatatatc
     3841 aaacgaccat ccgcgatcgt ctgacagatc cggcaaggta ttgtcgcgcg gtgcaaccgg
     3901 agcgtcttca ctgcgcaccg cttccgtctg cggcgtcagt tggatcactg ccccttttag
     3961 catcacctgc ttaacgctca gttgatgact cagtagtggt aaaagcgcca cgtccaggcg
     4021 catgttgtcg gcgcgaacca gtggctggct cgcgccctgg gcggtgagag acattcgccc
     4081 ggagaggata ctaagctgcg gccagacgtg ccaacgcagt ggcccgtcga gctgcaattg
     4141 ataaccgcta cgtgcagcaa cttgcttgac catatagtcg cggaaatcat tcggattcac
     4201 cagcaacact aacgcagata acccggccac cagcacgacc aggagtatca tcagcgtcgt
     4261 cagaaatcgt ctcatggtat cctcaatggg cctgaattag tctttatcga ttcggctggc
     4321 taccgcgccc tgctggttgc gatacttcgc atcttcacgg cggttgtaag gtcgcgccgc
     4381 cgggccggaa agcggctcaa agctcagcgc accaattaac atgcccggac gcagcgccag
     4441 cggcagctta ccggagttgt agaactccag cacaatgcaa ccagaccagc ccggatcgat
     4501 acggtgcgcg gtgacgtgca ccatcagccc cagacgcgcc agtgaggaac gcccgtccag
     4561 ccagcccacc agatcggctg gcagcgtcac cgactccagc gtcaccgcca gcgccagctc
     4621 tcctgggtga agatagaacg cctcgctttc atcgagaacg atctcatcgc tcatcacgcg
     4681 gtcaagcgcg gcgctcactt catctttggg accgctcaga tcgataaacg ctgccgtgtg
     4741 accacggaag gtacgaaatt tattgcccag gcgtacatcc accgtcgcgc cgttaatacg
     4801 ctccactggc ggacgtgggt tgatcgacaa acggccttca tcaagccagg cttcaatatc
     4861 tcggtcacac agacgcatgg cattttctcc tttcgcgcat cactccctta acgccaatta
     4921 cgtcaagggc ataccaggtt atcactgaac ggtacacaat ttatcaagct tattcaaaga
     4981 actgacttat tttcgctttc aatatatcga tcgcgatgcg gtttttcccg ccgcgcggca
     5041 cgataatgtc cgcatattgt ttagaaggct caatgaattg caggaacatc gggcgcacgg
     5101 ttttttgata ttgcgccatc acggaatcca ttgaacgccc acgctcgtta acgtcacgct
     5161 tgatgcggcg catcaggcag atatccagcg gggtatcaac gaaaatggag aagttaagtt
     5221 cgtcacgcaa acgtgcatcc gtcagcaaca aaatgccttc gagaatgatg accttcttcg
     5281 gctcaaccgt caccgtttct ttcatacgcg tatgttcaac atagctgtaa accggcaggt
     5341 caattgccga gccgcgtttc aacgcttgta aatgctcaag cagcaggctg tgatccatcg
     5401 cgctgggatg gtcgtagttg gtcttaacgc gttcttccat cgacagatgg ctttgatctt
     5461 tgtaatagca gtcttcggga attacgccga tgtgttcatc accgacttgc tcacgtaatt
     5521 cacgataaag ggtactggca ataagactct tgccggaagc cgatgcgcca gcgataccga
     5581 taatgacgca ctgatgagac tgatcagtca tatatttagc gacctgatta acctggatgt
     5641 taggaagggg gcgacgaagc gccaaacgcg gcaattatag ggatttcatc cgcctgatac
     5701 cagtcgaata gcgttgccgc gcgctcagag ttaattgttg acgaagaatt cccggtggca
     5761 aattacgttg atcagtttta tacaaggtaa aaattgttat acgcagttgc gcaaattatc
     5821 cgcctttacg tcactttatg agcaattcgc atataaaatg taaaactttt gtactagcat
     5881 aaacacagaa acgaatactg gcgacctggc cttgcggata aagcggtaat gagcaaacaa
     5941 tcacagcatg tattaattgc cctgccccac ccgctgcttc acctggtcag tttaggttta
     6001 gtctcgttta tcttcaccct tttctcgctt gagctttcgc agtttggcac ccaactcgcc
     6061 ccactgtggt tcccgacgtc catcatgatg gtggcgtttt atcgccatgc cgggcgcatg
     6121 tggccaggga ttgcgctgag ctgctcgctg ggaaatatcg ccgcatccat cctgcttttt
     6181 tccaccagct cgctgaacat gacctgcacg accatcaata ttgttgaagc cgtggtcggg
     6241 gcagtgctgc tacgtaaatt gctgccgtgg tataacccct tgcaaaatct ggctgactgg
     6301 ctgcgtctgg cactcggcag cgccattgtt ccacctctgt tggggggtgt tctggttgtc
     6361 ctgctgacgc ccggagacga tcctcatcag ggcatttttg atatgggtac tgtcagaatc
     6421 catcggcgct ctggcactgg tgccgctggg attgttattt aaaccacact atctgctgcg
     6481 ccatcgcaac ccacggttgc tttttgagtc gctgctcacg ttagccatca cactgacgtt
     6541 aagctggctt tcgatgctgt atctgccgtg gccttttact ttcattattg tgctgttgat
     6601 gtggagcgcc gtgcgcctgc cacgaatgga agcctttttg atcttcctta ccacggtgat
     6661 gatggtgtcg ctgatgatgg ccgcggatcc ctccctgctt gctacgccgc gtacgtacct
     6721 gatgagccat atgccgtggc taccgttttt gctgatcctg ctgcccgcca acatcatgac
     6781 gatggtgatg tatgcctttc gtgcggaacg caaacacatt tccgaaagcg aaacccgttt
     6841 tcggaacgcc atggaatatt ccgccatcgg catggcatta gtgggcaccg agggacaatg
     6901 gctgcaatcc aacaaagcac tctgccagtt tctcggttac agtcaggaag agctgcgcgg
     6961 actcaccttt cagcaactga cctggccgga ggatctcaat aaagatctcc aacaggttga
     7021 aaagctgata agcggtgaaa taaacaccta ttcaatggaa aaacgttact acaaccgcaa
     7081 tggcgatgtt gtctgggcgt tgcttgccgt ctctctggtg cgccacacgg atggcacgcc
     7141 gctctatttt atcgctcaga ttgaagacat taacgagcta aaacgcaccg aacaggtgaa
     7201 tcagcaactg atggaacgca tcacgctggc taacgaagcg ggcgggattg gcatctggga
     7261 gtgggaactg aagccgaata tttttagctg ggataagcgg atgttcgagc tgtatgaaat
     7321 tcctccgcat atcaaaccga actggcaggt gtggtacgag tgcgtgctgc cggaagatcg
     7381 ccaacacgcc gaaaaagtga ttcgtgattc gttgcaatca cgctcgccct ttaaactgga
     7441 atttcgcatt gccgtgaaag atggcatccg ccatatccgc gccctcgcca accgggtact
     7501 gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga ctgaggttaa
     7561 acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc tggactccat
     7621 cggcgaagcc gtggtctgta ttgatatggc gatgaaaatt acctttatga atccagtggc
     7681 ggagaagatg agcggctgga cgcaggaaga agcgttaggt gttccgctcc tgacggtgtt
     7741 gcatattact tttggcgaca acggaccatt aatggagaac atttacagtg ccgacacctc
     7801 acgttccgcg atcgaacaag atgtggtgtt gcactgtcgg agcggcggca gctacgacgt
     7861 gcattacagt attacgccgt taagtactct ggacggcagc aatataggct cggtactggt
     7921 gattcaggac gttaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca
     7981 tgatgcactg acgcatctcg ccaaccgcgc cagttttgag aaacaactgc gtatcctgct
     8041 gcaaacggta aacagtacgc atcagcgaca tgcactggtg tttatcgatc ttgatcgctt
     8101 taaagcggtg aatgacagcg ccgggcatgc ggcgggcgac gctttgctgc gcgaactggc
     8161 gtcgttgatg ctgagtatgc tgcgctccag tgacgtgctg gcgcggctcg gcggtgatga
     8221 atttggtctg ctgctgccag actgcaatgt tgaaagcgct cgttttatcg ctacacgtat
     8281 tatcagtgct gtgaatgact atcactttat atgggaaggc cgggtacatc gggtaggtgc
     8341 cagcgccggg attaccttga tcgatgacaa caatcatcag gcggcagaag tgatgtcgca
     8401 ggctgatatc gcctgttatg cctccaaaaa tggtggccgg ggccgggtga cggtttacga
     8461 accgcagcaa gctgccgcac atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg
     8521 gatgattaaa gagaatcagt tgatgatgct cgcccacggt gtcgcttcgc cacggatccc
     8581 ggaagcgcgt aatttgtggc tgatttcact taagctctgg agttgcgaag gcgagattat
     8641 tgatgaacaa acatttcgtc gtagcttcag cgatccggcg cttagccatg ctcttgaccg
     8701 ccgggtattc cacgaatttc tccagcaggc cgcaaaagcg gttgccagta aaggcataag
     8761 catcgccctc cccctttccg ttgccggttt gagtagcgcc acgctggtga atgatctgct
     8821 tgagcagctg gaaaatagcc ctctaccacc acggttatta catctgatta ttccggcaga
     8881 agcgattttc gatcacgcag aaagcgtgca aaaactgcgg ctggggggat gccggatcgt
     8941 attcagtcag gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga atatggcaga
     9001 ttacctgcta cttgatggtg agttatgcgc caacgtgcag ggtaatttga tggatgagat
     9061 gctgattacg attattcagg ggcacgctca gcgactcggg atgaaaacca tcgccgggcc
     9121 agtcgtttta cccttagtga tggatacgct ttctggcatc ggcgtcgatc tgatttatgg
     9181 tgaggtgatt gccgatgccc aaccgctgga tttgctgatg aatagtagtt atttcgcgat
     9241 taactgaggg agcgtttgcc cattgcctga tgcgacgcta acgcgtctta tcaggcctac
     9301 aaatcgctca ttccgcaagg cgctcgcacc gcatccggcg accaacgtca tgcttcgtct
     9361 ggttgccagc cttccgtata ccagatatgc aacagcgcat aagaacgcca gggcttccag
     9421 cgctcggcat aacggcggat ttgcgccggt gtcattcccg gaaatcgctg tttaatcaga
     9481 taatcatccg gcaggaaaac atctttcgcc tgccagccac gcaaagcaaa ataattcgcc
     9541 gtccagcgcc cgatacccgg aaaagtttgc agcgttttca tcgcctgctc cacatcgccc
     9601 ggtattgtca ttggtaaggt gccctccagc gccgcatttg ccagatgaat cagcgcctct
     9661 gcccgtttca acggcatacc taacgctttt aatgcctgcg ggtctgctgc tgccagccgc
     9721 tgaggcgtgg ggaagcagat atactccgga aaatcatcca gccgttcgcc ataaagctgt
     9781 accactttgg cagtcaattt tgccgccatc gccacgctca ccagttggcc taaaatcgcc
     9841 cgcacgcctt gctcaaaagc atcaacactg ccgggtaaac gcaatccggg ccgcgccgcg
     9901 cctaacttgc ccaacgcacc gttaacaatc tgcgggttac attgcagatc aagcaggcgg
     9961 ctcattttcg ccagacactc agcggcaacg ggttctaaat ctgcacttaa atttatgtgc
    10021 agagtatggc gggctatatc cggaatagca gtcaccacgc cgcgatattc gcccaccgcc
    10081 agactacggg cataataatc gtcagcgacc gtttccacac cactcactgc acgggcggcg
    10141 agaaatccca acatccacga ccagtcatac ggcggctgcc agttcagggt atacatcgca
    10201 tctccttatt catccgcttt cagcataaac gttattcaga cgctgcgctt tgctttcata
    10261 ttccggttgt cgcgacggca acatttcgct aaagtcacgc cccttcttca ccggcatggg
    10321 gattatttcg tgtttattgg ttttgattac ggtacagcaa actgttcagt agcggtcatg
    10381 cgtgacggta aaccgcactt gctaaaaatg gaaaacgaca gcacgctgct gccttcaatg
    10441 ctttgcgcgc caacgcgtga agcggtaagc gaatggctgt accgccatca tgatgttccg
    10501 gcagacgacg atgaaacgca ggcactgttg cgtcgggcga ttcgttataa tcgcgaagaa
    10561 gatatcgatg ttacggcgaa aagcgtgcag ttcggtcttt cctcactggc acagtacatt
    10621 gatgatccag aagaagtgtg gtttgtgaaa tcaccaaaat cgttcctcgg tgccagcggc
    10681 ttaaaaccgc agcaggtagc gctgtttgag gatctggtct gcgcaatgat gttgcacatt
    10741 cgccagcagg cgcaagcgca gctgccagaa gcgattactc aggcggtgat tggtcgcccg
    10801 atcaacttcc aggggctggg cggcgatgaa gcaaacgccc aggcgcaagg gattctggaa
    10861 cgcgcggcga agcgtgccgg attcagggac gtggtattcc agtacgagcc ggtagcggct
    10921 gggctggatt acgaagccac cttacaggaa gaaaaacggg tactggtggt ggatatcggc
    10981 ggtggtacga ctgactgttc attgctgctg atggggccgc agtggcgttc gcgtctcgat
    11041 cgtgaagcca gcctgctggg tcacagtggt tgccgtattg gcggtaacga tctggatatc
    11101 gcactggcgt ttaaaaacct gatgccactg ctgggtatgg gtggcgaaac cgaaaaaggc
    11161 atcgccctgc cgattctgcc gtggtggaat gcggttgcca tcaacgacgt acctgcgcag
    11221 agtgatttct acagtagtgc caacggtcgt ctgcttaacg atctggtacg cgatgcccgc
    11281 gaaccggaaa aagtggccct gctacagaaa gtctggcgtc agcgtttaag ctatcgcctg
    11341 gtgcgtagcg cagaagagtg caaaattgcg ctttcaagcg tagcggaaac ccgcgcctca
    11401 ctgccgttta tcagcgatga actggctacg ctgattagcc agcaagggct ggaaagcgcc
    11461 ctcagccagc cactggcgcg gattcaggaa caggtgcaac tggcgctgga taacgcccag
    11521 gaaaaaccgg acgttatcta cctgaccggc ggaagcgccc gttctccgct gattaaaaaa
    11581 gcgctggcag aacagttgcc gggcattccg attgcaggcg gcgatgactt tggctccgtc
    11641 accgccgggc tggcacgctg ggcggaagtg gtgtttcgtt aattgaaaaa tggctcagca
    11701 gcggcttaaa tctgcctgcg cctgagccag tttttctgcc gcctgacgta acggttccat
    11761 caacggcatg gtccgtgctg gagcatgaag gcggaatcgc tgcaactctg tcgcgccgac
    11821 agtcaacgcc gattccagcc gatgtcgttt ggcggccatt ttcgccatta ccgcttgtct
    11881 ttctgcaggc gttaccgctt cgttcggcct gaacacaaaa cggcgttcac agctcgcttt
    11941 ccagtcgata accgcctggg tcagatgatc accaaaccct ttaacttgct taaccccacg
    12001 acgggtaaca tccgcggctg tttcaatacc aaaagaccgt aacgccgctt tacgcgcagg
    12061 gccaacgccg ggaatagagg cagcatcaat aaaaaatccc tccagaaact tctgcttctg
    12121 ccgttccctt gcagtgtcat gaagtgctgc cagagcgcgt ttctcctctt ccggtaaccc
    12181 gagaatttcg tccttcattt tttcgagcat cgcccgtttg gcgataaaac cttccagccc
    12241 gcccaactgt tggatctggc tgactaaatg gtcatagtcc attttcgcgc gattaaatgc
    12301 ctcacggcgt tgctggattt ctgctttata aactttgctt gtcagactgc caataatcca
    12361 gatagccgcc agcacaggaa ccaaaataat ataacgcggt tctgcctgaa ggccgcaaag
    12421 caacagcgat aacgctgaca gtgcgatctc aatcagaatg atgtattcgc gccgtaacag
    12481 gcctaaagga agcggcctgc cagtcggttg gaaatgatcg ggtaatggca attgcaatgc
    12541 tggcggtgct actgacgcca tcaccatcgc ccagactttc gccagcacaa aatcaccact
    12601 ggtggtaatg acctcttcgc cgagatcaat aaaatagata acgccttgat tatccagcgc
    12661 acaccacggg cagtcagtca aatgaccggg gtaaacatgc attgccgaaa cggtacattt
    12721 ctttaactgt tggcgtagta aatccagtgc cgctacccac gcttttgccg tcggacgcgc
    12781 ggttgccacg ccactttccg tgaatgcctg ctgaaacatg gcttcaacat cgcccggtaa
    12841 catcgacagc ggaatcgatc gtggcggcgg ttttaaacca cgtcgctggt tgtctgacgc
    12901 gtaggcataa cggaaatggg caatatccgt ctccagcgca ttacccgcat cagagataag
    12961 cggcacacca gaataaggat gccgcccacc aaacaagacg tgaaaaatca gcaacgcaag
    13021 gccaaaatta tcgtgattcg cggtacgttc aaaaccgaca aatgatggca aggtttgtag
    13081 ctctggcggc gtaaaatgcg acacgccgac ttcgcataaa tgcagtgtgc cattggcgtt
    13141 aatctgaaag gagtcactat cgatcaacac cactttgctg tcgcgaccta ccataaagct
    13201 gttctggttt acgtccccca cgacgtgccc gtgctcgtga accgtagcaa aagatgaagc
    13261 aatattgcgc gcaacataga gtagaaaatc ccacgcacaa tgaggataac tctggcgacg
    13321 atgtgccggg ctatagatca tatgaatcgg ttctttacca gaaacttttg gcatcataaa
    13381 accgataact tttccgcctc gtccaccgtg aagcgttgcc tgcggccagg cgacataatt
    13441 caacaactgc gcgtcagctg tcgcagccat aaaggcaagt ttgtcctgtt ttaaggcggg
    13501 tggcggcgtg tgataaatct tggcgacgct atcgacaaac tcattgatat cataaaccgc
    13561 gccttcgcca cctttgccca gttcacggcc tggcgtaacg cactcaccag tagcagtata
    13621 taaagtgggt ttcatggtaa ccacagcgcc agtgtcaacg ttttatcatc atcggtacgc
    13681 tcgtttaccg ctgggctcga caaaaactgc tttaacaatt caggtaataa atcgagttgt
    13741 tcctgcgttg ctgccgccag tccattgaaa aacggggtaa agaaaggcac atgcggagaa
    13801 ttatccagca tatttaacgc cagtcgctga ataccatccg taaatgctgc aactttatgc
    13861 gcacgctcag tgctggtgaa agtttccagt ctggaaacga catcttcatc ggtaatgaaa
    13921 tgcgtcatat tggcatattc acccaccatc ggcgtaagcg gtaattgcag accgtgacca
    13981 aaatccacca ccacgccgcc atcgccaatc tgcataatca aggtgccgtt agccgatgaa
    14041 atcaatccca gaaacgtgca ggcaaaatcg cgaactgcta attctttagc ttccgcctcg
    14101 gcaaaaagtc gctgacgaac ggtcagcaca atatccgtgg cgagaatatc attgagtcca
    14161 aattcaccgt gctgcacttt ttgcgacaca taagccatcg cttcattgat ggcaagcatc
    14221 gcgccttcgc caccctgcga gacactgcct gcgccatcag caaggaacat caccagtaat
    14281 ggctgctggt catttagcca ggcaacctgc atctggcagg catcctgaca aggtaaatca
    14341 gcgctaatgt gcgacgttcc aacagcggat gcataaacca gacgccagct cacactgacg
    14401 tccagccttt tggcgcttcc agcacaactt ccgttcccgg cgtggagcgg gaaaccgaac
    14461 gcagcgaact ggataaccag ctaaacagtt cacggaattg taatccctgc agtggcaaag
    14521 gctgacgaac gctgatttgt gccagagtct taatatccgc cccctgaaca ccgatggaaa
    14581 agaaggcaaa cttcttatct tcttccccct gaaacacttt gttggcggca gcctgccact
    14641 catcggttgg tgcaccatcg gtgatcagga aaatccacgg gcgataatac gaaataccat
    14701 ttgcacgata ctcacgtttc cgctcctcta ccatatccag ggctttggta atggcggcac
    14761 ccattggtgt atccccctgg gcaaacagga tgggcgggaa aaaattagcg gcgctggtaa
    14821 agggttgttc cacatgcacc gggccgaacg tcacaatacc aagttccact ctttttaagg
    14881 ccagcgggtc ggcaagcagc tcatcgcgaa aggtaaccaa tccggcgtta agttcattaa
    14941 tgggtctacc gttcatcgaa ccagaaacat ccagcagcaa aatgcagggg caacgtggtt
    15001 ccgggttact ggcaaaatcg ctggtggcaa acgtgatttg ttcgctcata agaaatcctt
    15061 tcttagagaa ttattgaggt ccggtatcat accgattgca ataatgaaat aagagcaaac
    15121 gaagaaactt tcagaattag gcccggcggc gcagaaaatt ttgctattcg cctcgcaaga
    15181 acggagagcc gtcgattgac actaagggcg gagtgacata atttcaggag tgagggttag
    15241 ggaaaggttt ccccctcccc ctggtgttct tagtaagcct ggaagctaat cactaagagt
    15301 atcaccagta tgatgacgtg cttcatcata acccttttct tatttaaggc cccttcctcg
    15361 ggaggggctt tcccgtttca gcatcccgct gaaatcgtcg gcttacctcc tttcgccatg
    15421 catgcagtct atcgctgact tgcggataaa atagtttcca gtgtgattat tgcgtaacgt
    15481 ctcgcaaaga atatttgctg gcaggatcgc agactgcaaa gcctgcagat tgacaatctt
    15541 atcgtgaagg catactttca ggagtgaggg tagagcgggg tttcccccgc cctggtagtc
    15601 ttagtaagcc gggaagctta tgactaagag caccacgatg atgatccact tcatcatgac
    15661 cctttcctta tttaaggccc cttcctcggg aggggctttc ccgtttcagc gtcccgctga
    15721 aatcgtcggc ttacctcctt tcgccctgaa ttcagtctat cgctgacttg cggataaaat
    15781 agtttcctct gtgattattg cgtaacgtct cgcaaagaat atttgctggc aggatcgcag
    15841 actgcaaagc ctgcggattg acattctgaa cgtgaaggca tactttcagg agtgagggta
    15901 gagcggggtt tcccccgccc tggtagtctt agtatgctgg gtagctaatc actaagagca
    15961 ccacgatgat gagtatcttc atcatgaccc tttccttatt tatggcccct tcctcgggag
    16021 ggggctttcc cgtttcagcg tcccgctgaa atcgtcggct tacctccttt cgccatgcaa
    16081 gcagtctatc gctaacgcgt agataaaata gtttcctgtg ttattactgg atgcgtgctc
    16141 gcaaatgtgc ccgccattca gacgattccc gatagtgttt cataattcct ccatttttct
    16201 cccttattgg ctggctacac tagtatcatt ccgcgaaacg tttcaggaag agaaactctt
    16261 aacgatgaaa ggcagttata aatcccgttg ggtaatcgta atcgtggtag ttatcgccgc
    16321 catcgccgca ttctggttct ggcaaggccg caatgactcc cagagtgcag ccccaggggc
    16381 gacgaaacaa gcgcagcaat cgccagcggg tggtcgccgt ggtatgcgtt ccggcccatt
    16441 agccccggtt caggcggcga ccgccgtaga acaggcagtt ccgcgttacc tcaccgggct
    16501 tggcaccatt accgctgcta atactgttac ggtgcgtagc cgcgtggacg gtcaactgat
    16561 ggcgttacat ttccaggaag gccagcaggt caaagctggc gatttactgg cagaaattga
    16621 ccccagccag ttcaaagttg cattagcaca agcccagggc caactggcaa aagataaagc
    16681 cacgcttacc aacgcccgcc gtgatctggc gcgttatcag caactggcaa aaaccaatct
    16741 cgtctcccgc caggagctgg atgcccaaca ggcgctggtc agtgaaaccg aaggcaccat
    16801 taaggctgat gaagcaagcg tcgccagcgc gcagctgcaa ctcgactgga gccgtattac
    16861 cgcaccagtc gatggtcgcg ttggtctcaa gcaggttgat gttggtaacc aaatctccag
    16921 tggtgatacc accgggatcg tggtgatcac ccagacgcat cctatcgatt tgctctttac
    16981 cctgccggaa agcgatatcg ctaccgtcgt gcaggcgcaa aaagccggaa aaccgctggt
    17041 ggtagaagcc tgggatcgca ccaactcgaa gaaattaagt gaaggcacgc tgttaagtct
    17101 cgataaccaa atcgatgcca ctaccggtac gattaaagtg aaagcacgct ttaataatca
    17161 ggatgatgcg ctgtttccca atcagtttgt taacgcgcgc atgttagtcg acaccgaaca
    17221 aaacgccgta gtgatcccaa cagccgccct gcaaatgggc aatgaaggcc attttgtctg
    17281 ggtgctgaat agcgaaaaca aggtcagcaa acatctggtg acgccgggca ttcaggacag
    17341 tcagaaagtg gtgatccgcg caggtatttc tgcgggcgat cgcgtggtga cagacggcat
    17401 tgatcgcctg accgaagggg cgaaagtgga agtggtggaa gcccagagcg ccaccactcc
    17461 ggaagagaaa gccaccagcc gcgaatacgc gaaaaaagga gctcgctcct gatgcaagtg
    17521 ttacccccga gcagcacagg cggcccgtcg cgcctgttta ttatgcgtcc tgtggccacc
    17581 acgctgctga tggtggcgat cttactcgcc gggattatcg gttatcgcgc cctgcccgtt
    17641 tcggcgctgc cggaagtgga ctatccgacc attcaggtgg tcacgctcta cccaggtgcg
    17701 agcccggatg tcatgacctc tgccgttacc gcgccgctcg aacgccagtt cgggcagatg
    17761 tctggcctga aacagatgtc gtcgcaaagt tccggcggtg cgtcagttat cactttacag
    17821 ttccagctaa cattaccgct ggatgtcgcc gagcaggaag tgcaggccgc gattaacgct
    17881 gcgaccaact tgttgccgag cgatctgcct aacccgccgg tttacagcaa agtgaacccg
    17941 gcagatccgc cgatcatgac gctcgccgtc acctcaacag ccatgccgat gacgcaagtg
    18001 gaagatatgg tggaaacccg cgtcgcgcag aaaatctcgc agatttccgg cgtcggcctg
    18061 gtgacgcttt ccggcggcca gcgtccggct gttcgcgtca aacttaacgc tcaggcgatt
    18121 gccgccctcg gcctgaccag cgaaaccgtg cgcaccgcca ttaccggcgc taacgttaac
    18181 tcggctaaag gtagccttga cggcccttcc cgtgcggtta cgctttccgc taacgaccag
    18241 atgcaatccg ccgaagagta tcgccagcta atcatcgcct accagaacgg cgcgccgatt
    18301 cgtctgggcg atgtcgcaac tgtagagcaa ggtgcagaaa acagctggct cggcgcatgg
    18361 gcgaataaag aacaggccat tgtgatgaat gttcagcgcc agcccggtgc taacattatc
    18421 tccaccgccg acagcatccg gcagatgctg ccacagctca ccgagagtct gccgaaatcg
    18481 gtgaaggtga cggtactttc tgaccgtacc accaatatcc gcgcatccgt cgatgatact
    18541 cagtttgaat tgatgatggc tatcacgctg gtagtcatga ttatctacct gtttttgcgc
    18601 aatattccgg cgaccatcat tcccggcgtt gcagtgccgc tgtcgttaat cggcaccttc
    18661 gcggtcatgg tgtttctcga tttttcaatc aataacctga cgctgatggc gttaactatc
    18721 gccaccgggt tcgtggtcga tgacgccatc gtggtgatcg aaaatatttc ccgctatatc
    18781 gaaaaaggcg aaaaaccgtt ggcggcggcg ctcaaaggcg caggtgaaat cggctttacc
    18841 attatctcgc tgaccttctc gctgattgcg gtgttgatcc cactgctgtt tatgggcgat
    18901 atcgtcgggc gactgttccg cgaatttgct attaccctgg cggtagcgat tttaatctca
    18961 gccgtggtgt cgctaaccct gacaccgatg atgtgcgcac ggatgctcag ccaggagtcg
    19021 ttgcgtaaac agaaccgctt ctcccgtgcc tcggaaaaaa tgttcgacag gataatcgcc
    19081 gcctatggtc gtggactggc gaaagtgctg aatcatccgt ggctgacctt aagcgtggcg
    19141 ctcagcacgc tgctgcttag cgttctattg tgggtgttca ttccgaaagg tttcttcccg
    19201 gtacaggata acggcattat tcagggcact ttgcaggcac cgcaatccag ctcctttgcc
    19261 aatatggccc agcggcaacg ccaggtcgcg gacgtgattt tgcaggatcc ggcagtgcaa
    19321 agtctgacct catttgttgg cgttgatggc actaacccgt cgctgaacag tgcgcgtttg
    19381 cagatcaacc tcaaaccgtt ggatgaacgt gatgatcggg tgcaaaaagt catcgcccgt
    19441 ctgcaaacgg cggtggataa agtgccgggc gtcgatctct tcctgcaacc aacgcaggac
    19501 ctgaccatcg atactcaggt cagccgcacc cagtaccagt ttaccttgca ggccacgtca
    19561 ctggatgcgc tcagtacctg ggtgccacag ttgatggaaa aactccagca gctgccacag
    19621 atttctgatg tctccagcga ctggcaggac aaagggctgg tggcgtatgt caatgttgat
    19681 cgcgacagcg ccagccgtct ggggatcagc atggcggatg tcgataacgc cctgtacaac
    19741 gcgtttggtc agcgactgat ttccactatt tatactcagg ctaaccagta tcgtgtagtg
    19801 ctggagcaca ataccgaaaa taccccaggc ctcgcggcgc tggataccat tcgcctgacc
    19861 agcagcgacg gcggcgtggt gccgctaagc tcaattgcca aaattgagca gcgttttgcg
    19921 ccgctttcca tcaaccatct ggatcagttc ccggtaacga ccatctcctt taacgtgccg
    19981 gataactatt cgctgggcga tgcggtgcag gcaattatgg acaccgagaa gacgctgaat
    20041 ctgccggtgg atatcaccac gcaattccag ggcagcaccc tcgccttcca gtcggcgctg
    20101 ggcagcactg tctggctgat tgtcgcggcg gtggtggcga tgtatatcgt gctcggcatt
    20161 ctgtacgaga gctttattca cccgatcacc attctctcga cgctacccac ggcaggtgtt
    20221 ggcgccctgc tggcgttaat gattgccggt agcgaactgg atgtgattgc gattatcggc
    20281 attattttgc tgatcggtat cgtgaagaag aacgccatca tgatgatcga cttcgcgctg
    20341 gctgctgagc gcgagcaagg catgtcgccg cgcgatgcaa tctaccaggc ttgtctgttg
    20401 cgttttcgtc cgatcctgat gaccaccctg gcggctctgc ttggcgcgct gccgctgatg
    20461 ttgagtaccg gggtcggcgc ggaactacgt cgtccgttag gtatcggcat ggtcggcggc
    20521 ctgattgtca gccaggtgct gacgctgttc accacgccgg tgatttatct gctgtttgac
    20581 cgcctggcat tgtggaccaa aagtcgcttt gctcgccatg aagaggaggc gtaagtgaag
    20641 ttttttgccc tcttcattta ccgcccggtg gcgacgattt tactgtcggt cgccattacc
    20701 ctgtgcggca tactgggttt ccgtatgctg ccggtcgccc cgctgccgca ggtcgatttt
    20761 ccggtgatta tggtcagcgc ctcgctgccc ggtgcgtcgc cggagacaat ggcgtcttcc
    20821 gttgccacgc cgctggagcg ctcacttggg cgcattgccg gggtcagtga aatgacctcc
    20881 agcagttcgc tcggcagcac gcgtattatt ttgcagtttg attttgaccg ggatatcaac
    20941 ggtgcggcgc gtgatgtgca ggcggcgatc aacgccgcac aaagtctgct gcccagtggg
    21001 atgcccagcc gcccgactta tcgcaaagcg aacccgtcgg atgcgccaat tatgatcctc
    21061 acgctaacct ccgatactta ttcgcagggt gaactgtacg atttcgcctc gacgcagctg
    21121 gctccgacga tttcgcaaat cgacggtgtc ggtgatgtcg atgtcggcgg cagctcgctg
    21181 cccgccgtgc gcgtcgggct gaacccgcag gcgctgttta atcagggcgt gtcgctggac
    21241 gacgtacgca ctgccatcag caatgccaac gtgcgtaaac cgcagggggc gctggaagat
    21301 ggcactcacc gctggcagat ccagaccaac gatgaactga aaaccgccgc tgaatatcag
    21361 ccgctgatta ttcactacaa caacggcggc gcggttcgtc tgggcgatgt ggcgacggtg
    21421 accgactcag tgcaggatgt gcgcaacgcc gggatgacca acgccaaacc ggctatttta
    21481 ctgatgatcc gcaaattgcc ggaagccaat attatccaga cggtagacag catccgggca
    21541 aaattaccgg agttgcagga aaccattccg gcggcgattg atctgcaaat tgcccaggat
    21601 cgctccccca ccattcgcgc ctcgctggaa gaagtcgagc aaacgctgat tatctcggtg
    21661 gcgctggtga ttctggtggt ctttttattc ctgcgctcgg gtcgcgccac tattattccc
    21721 gccgttgcgg tgccggtttc gctgattggt acgtttgcgg cgatgtacct gtgcggattc
    21781 agtctcaata acctttcgtt aatggcgctc accatcgcta ctggtttcgt ggtggatgac
    21841 gccatcgtgg tgctggaaaa cattgcacgt catctggaag cgggaatgaa accgttgcaa
    21901 gccgcactgc aaggtactcg cgaagtcggt tttacggtgc tgtcgatgag tctgtcgctg
    21961 gtggcggtgt tcctgccgct gctgttgatg ggcggattgc cgggccgact gttacgcgag
    22021 tttgccgtga cgctttctgt cgccattggt atttcgttgc tggtttctct gacattaacg
    22081 ccaatgatgt gtggctggat gctgaaagcc agcaagccgc gcgagcaaaa gcgactgcgt
    22141 ggttttggtc gcatgttggt agccctgcaa caaggctatg gtaagtcact gaaatgggtg
    22201 ctcaatcata cccgtctggt gggcatggtg ctgcttggca ccattgcttt gaatatctgg
    22261 ctatatatct cgattccgaa aaccttcttc ccggagcagg acactggcgt gttgatgggc
    22321 gggatacagg cggatcagag tatttcgttt caggcgatgc gcggtaagtt gcaggatttc
    22381 atgaaaatta tccgtgacga tccggcagtg gataatgtca ccggctttac cggcggttcg
    22441 cgagtgaaca gcgggatgat gtttatcacc ctcaagccac gcgacgaacg cagcgaaaca
    22501 gcgcagcaaa ttattgaccg tctacgcgtg aagctggcga aagaaccggg ggcgaatctg
    22561 ttcctgatgg cggtacagga tattcgcgtt ggcggacgtc agtcgaacgc cagctaccag
    22621 tacacgttgt tatccgacga cctggcggca ctgcgcgaat gggagccgaa aatccgcaaa
    22681 aaactggcga cattgccgga actggcggac gtgaactccg atcagcagga taacggcgcg
    22741 gagatgaatc tggtttacga tcgcgacacc atggcacggc tgggaatcga cgtacaggcc
    22801 gccaacagcc tgttaaataa cgccttcggt cagcggcaaa tctcgaccat ttaccagccg
    22861 atgaaccagt ataaagtggt gatggaagtg gatccgcgct atacccagga catcagcgcg
    22921 ctggaaaaaa tgttcgtaat caataacgaa ggaaaagcga tcccgctgtc gtatttcgct
    22981 aaatggcaac cggcgaatgc cccactatcg gtgaatcatc agggattatc ggcagcctcg
    23041 accatctcgt tcaacctgcc gaccggaaaa tcgctctcgg acgccagtgc ggcgatcgat
    23101 cgcgcaatga cccagcttgg tgtgccttcg acggtgcgcg gcagttttgc cggcacggcg
    23161 caggtgttcc aggagacgat gaactcacag gtgatcctga ttatcgccgc catcgccacg
    23221 gtgtatatcg tgctgggtat cctttacgag agttacgtac atccgctgac gattctctcc
    23281 accctgccct cggcgggcgt tggagcgctg ctggcgctgg agctgttcaa tgccccgttc
    23341 agcctaatcg ccctgatagg gatcatgcta ttaatcggca ttgtgaagaa aaacgccatt
    23401 atgatggtcg attttgcgct tgaagcccaa cggcacggta acctgacgcc gcaggaagct
    23461 attttccagg cctgtctgct gcgttttcgc ccgattatga tgactaccct ggcggcgctg
    23521 tttggcgcgc tgccgctggt gttgtcgggc ggtgacggct cggagctgcg gcaacccttg
    23581 gggatcacca ttgtcggcgg tctggtgatg agtcagctcc tgacgctgta taccacgccg
    23641 gtggtatatc tctttttcga ccgtctgcgg ctgcgttttt cgcgtaaatc taaacaaacg
    23701 gtaaccgagt aaatgacaga tcttcccgac agcacccgct ggcaattgtg gattgtggct
    23761 ttcggcttct ttatgcagtc gctggacacc accatcgtaa acaccgccct tccctcaatg
    23821 gcgcaaagcc tcggggaaag tccgttgcat atgcacatgg tcattgtctc ttatgtgctg
    23881 accgtggcgg tgatgctgcc cgccagcggc tggctggcgg acaaagtcgg cgtgcgcaat
    23941 attttcttta ccgctatcgt gctgtttact ctcggttcac tgttttgcgc gctttccggc
    24001 acgctgaacg aactgttgct ggcacgcgcg ttacagggcg ttggcggtgc gatgatggtg
    24061 ccggtcggca ggttgacggt gatgaaaatc gtaccgcgcg agcaatatat ggcggcgatg
    24121 acctttgtca cgttacccgg tcaggtcggt ccgctgctcg gtccggcgct cggcggtctg
    24181 ctggtggagt acgcgtcgtg gcactggatc tttttgatca acattccggt ggggattatc
    24241 ggtgcgatcg ccacattgat gttaatgccg aactacacca tgcagacgcg gcgctttgat
    24301 ctttccggct ttttattgct ggcggttggc atggcggtat taaccctggc gctggacggc
    24361 agtaaaggta caggtttatc gccgctggcg attactggcc tggtcgcagt tggtgtggtg
    24421 gcactggtgc tttatctgct gcacgccaga aataacaacc gcgctctttt cagtctgaaa
    24481 ctgttccgca ctcgtacctt ttcgctgggc ctggcgggga gctttgccgg acgtatcggt
    24541 agcggcatgt tgccctttat gacgccggtt ttcctgcaaa ttggccttgg cttctcaccg
    24601 tttcatgccg gactgatgat gatcccgatg gtgcttggca gcatggggat gaagcggatt
    24661 gttgtacagg tggtgaatcg ctttggttat cgtcgggtac tggtagcgac cacgctgggt
    24721 ctgtcgctgg tcaccctgtt gtttatgact accgctctgt tgggctggta ctacgttttg
    24781 ccgttcgtcc tgtttttaca agggatggtc aactcgaccc gtttctcctc catgaacacc
    24841 ctgacgctga aagatctccc ggacaatctg gcgagcagcg gcaacagtct gctgtcgatg
    24901 attatgcaat tgtcgatgag tatcggcgtc accatcgccg ggctgttgct gggacttttt
    24961 ggttcgcagc atgtcagcgt cgacagcagc accacacaaa ccgtctttat gtacacctgg
    25021 cttagcatgg cgtttatcat cgcccttccg gcgttcatct ttgccagagt gccgaacgat
    25081 acgcatcaaa atgtagctat ttcgcggcga aaaaggagcg cgcaatgaag ttctggcgac
    25141 ctggtattac cggcaaactg tttctggcga ttttcgccac ctgcattgtc ttgctgatca
    25201 gtatgcactg ggcggtgcgt atcagttttg agcgtggctt tattgattac atcaagcatg
    25261 gtaatgaaca gcgattacaa ctgttaagtg atgcgcttgg cgagcagtat gcgcagcatg
    25321 gcaactggcg cttcctgcgc aacaatgatc gctttgtctt tcagatcctg cgttcatttg
    25381 aacacgataa ttcggaagat aaacccggcc cgggtatgcc accacacggc tggcgtactc
    25441 agttctgggt ggttgatcaa aacaacaagg tgctggttgg tccgcgagcg ccgattccac
    25501 ctgacggtac acggcgaccc attctggtca acggtgcgga agtgggcgcg gtgattgcct
    25561 cccctgttga gcggctgacc cgcaatactg atatcaattt cgataaacaa cagcggcaaa
    25621 ccagctggct gattgtcgcc ctggcaacgt tactcgcggc actcgctact tttctgctgg
    25681 cgcgcggttt gctggcaccg gtaaaacgac ttgtcgatgg cacgcacaaa ctggcggcgg
    25741 gcgatttcac tacccgcgta acgcccacca gtgaagatga actgggcaaa ctggcgcaag
    25801 acttcaacca gctcgccagc acgctggaga aaaaccagca aatgcggcgc gattttatgg
    25861 ccgatatttc tcacgaactg cgtacgccat tagcggtgct gcgcggtgaa ctggaagcca
    25921 ttcaggatgg cgtgcgcaaa ttcacgccgg agacggtggc gtctttacag gcggaggtcg
    25981 gtacactgac caaactggtt gacgatcttc atcagttgtc gatgtctgat gaaggcgctc
    26041 tcgcctatca aaaagcaccg gtagatttga tcccactgct ggaagtggca ggcggcgcat
    26101 ttcgcgaacg atttgccagc cgtggcctga aactgcaatt ttccctgcca gacagtatca
    26161 ccgtatttgg cgatcgcgac cgtttaatgc agttattcaa taacttactg gaaaacagcc
    26221 tgcgctacac tgacagcggc ggcagcctga aaatctctgc cgagcagcac gacaaaacgg
    26281 tgcgcctgac ctttgccgac agtgcgccag gtgtcagtga cgatcagcta caaaaattgt
    26341 ttgaacgttt ttatcgcacc gaaggttccc gcaaccgcgc cagcggcggt tccgggctgg
    26401 ggctggcgat ttgcctgaac attgttgaag cacataatgg ccgcattatt gccgcccatt
    26461 cgccttttgg cggggtaagc attacagtag agttaccgct ggaacgggat ttacagagag
    26521 aagtatgacc gagttaccaa tcgacgaaaa cacaccgcgt attttgatcg tggaagatga
    26581 accgaagctg ggacagttgc tcattgatta tctgcgtgct gcgagctatg cgccgacgct
    26641 tatcagccac ggcgatcagg tactgtcgta cgtgcgccag acgccgccgg atctgatcct
    26701 gttggatctg atgctccctg gcaccgatgg cctgacgctg tgccgggaaa ttcgtcgttt
    26761 ttctgacatt cctatcgtga tggtgacagc aaaaatcgaa gagatcgacc gcctgctagg
    26821 gctggagatt ggcgcagatg actacatttg caagccgtac agcccacggg aagtggtagc
    26881 gcgtgtcaaa accattttgc gccgttgcaa accgcagcgt gagttgcagc aacaggatgc
    26941 tgaaagcccg ttgattatcg acgaaggtcg ttttcaggct tcatggcgcg gtaaaatgct
    27001 tgacctgacg cctgcggaat ttcgtctgct gaaaacgctc tctcatgaac caggaaaagt
    27061 gttctcccgc gagcaattgc tcaatcatct ttatgacgac taccgcgtag taaccgaccg
    27121 taccatcgac agccacatta aaaacctgcg ccgcaagctg gaatctctcg acgccgaaca
    27181 gtcatttatc cgcgccgttt acggcgtcgg ttaccgctgg gaagccgacg cctgccgcat
    27241 cgtttagttt tagcgacatt actttgttag ccggagatgg cattcagacc agatccggca
    27301 acattatccc acgcatggtc agcaaactga cattatctct ccgccctggc aggcatcaga
    27361 aaatcgctca tactttaatc ggtaaacagc acctttagat gctgttttcg atacgcaatt
    27421 tcaatcaagg agtcattatg gctggttggt ttgaactcag taagagcagt gatagtcagt
    27481 tccggtttgt gctaaaagcg ggcaatggtg agacgatcct caccagcgag ctgtatacct
    27541 caaaagcctc tgcggaaaag ggcatcgcgt cggtgcgtag caacagcccg caagaagaac
    27601 gctatgagaa aaaaacggca agcaacggca aattctattt caatctgaaa gccgccaatc
    27661 atcaaattat cggctccagc cagatgtatg ccaccgcgca atctcgtgaa accggaattg
    27721 cctccgttaa agccaatggc acaagccaga cggtgaaaga caatacgtaa ttacgatgcc
    27781 gggcgtgggt tgttggcgtc cggcgcttta ccagatcccc cctacagcgc tacaatgccc
    27841 gcccttaaag tgggggcact cccctaaccg cttcatcagg tgaagcggat ctgacctgtc
    27901 atcagaacga gagaattatg tttaaaccgg aactcctttc cccggcggga acgctgaaaa
    27961 atatgcgtta cgctttcgct tatggcgcag atgctgttta tgcgggccag ccgcgttact
    28021 ccctgcgtgt gcgcaacaac gaattcaacc acgaaaacct tcagctcggc atcaatgaag
    28081 cccacgcgct ggggaaaaag ttttatgtcg tggtcaacat tgcaccgcac aacgccaagc
    28141 tgaaaacctt tatccgtgac ctgaaaccgg tggtggaaat ggggccggat gcgctgatta
    28201 tgtccgatcc agggctgatt atgctggtgc gtgagcactt ccctgaaatg ccgatccatc
    28261 tctcggtaca ggctaacgcc gtgaactggg cgacggtgaa attctggcag caaatgggac
    28321 tgacccgcgt gatcctctct cgcgagctgt cgctggaaga gattgaagag atccgcaatc
    28381 aggtgccgga tatggagatc gagatcttcg ttcacggcgc actgtgcatg gcctactccg
    28441 gtcgctgcct gctctctggc tatatcaaca agcgcgatcc gaaccagggt acctgcacca
    28501 acgcctgccg ctgggagtac aacgtccagg aagggaaaga agatgacgtt ggcaacatcg
    28561 tacacaagta cgagccgatt ccggtgcaaa atgttgagcc gacgctgggt atcggcgcgc
    28621 caaccgacaa agtgtttatg atcgaagaag cccagcgtcc gggcgagtat atgaccgcgt
    28681 ttgaagatga gcacggcact tacatcatga actcgaaaga tctgcgcgcc atcgcccatg
    28741 tagaacgcct gaccaaaatg ggcgtgcatt cgctgaaaat cgaaggccgt actaaatctt
    28801 tctactattg cgcacgcacc gcacaggttt atcgtaaagc tatcgatgat gccgctgcgg
    28861 gcaaaccgtt cgataccagc ctgctggaaa ctctggaagg tctggcgcat cgtggctata
    28921 ccgaaggttt cctgcgtcgt catactcacg acgattatca gaactacgaa tacggttatt
    28981 cagtttctga ccgccagcag tttgttggtg agtttaccgg tgagcgcaag ggggacctcg
    29041 cggcggtagc ggtgaaaaat aaattctccg ttggcgacag ccttgagctg atgacgccgc
    29101 aaggcaacat taactttacc cttgagcaca tggaaaacgc caaaggcgaa gctatgccgg
    29161 tagcaccagg cgatggttat actgtgtggc tcccggtgcc gcaggatctt gagctaaatt
    29221 acgcgctgct gatgcgtaat ttctccgggg aaactacgcg taacccccac ggtaagtgat
    29281 taatttcgat tatttttccc ggatggaaaa ttcttagaaa ccgatcacat acagctgcat
    29341 ttattaaggt tatcatccgt ttcgctgaaa aacataaccc ataaaatgct agctgtacca
    29401 ggaaccacct ccttagcctg tgtaatctcc cttacacggg cttatttttt acgcgtaata
    29461 caatgaaata aaaggattta tttctggtca cgtccacata ttgaccacat cgacaaaaaa
    29521 agcccctcaa ctgaggggct ttctgtttgt aattacatcc acataatttg ctgccctgac
    29581 ggcaacgggt gcggtcttac ggcgtggact tctcccggct tcacgatgta tcgctgtacc
    29641 gactcataag tgatgaacgt ggcgctgcaa ttcacgttct gacactggtg ataacgctct
    29701 tttgtcgtgt cagtgatata gcgacttgta cgcgcatgtg cggcatgctg gcataaagga
    29761 caatgaaaca tcgcgagcac ctcttccggt tttgttgatg gtgccatttt agttaattta
    29821 tccttataaa acaaacagat aaaataaaaa catcattcat catcttctgt ttcgtattcc
    29881 acatcagaaa gcctgacctc aagctctaag gacgtcgtga agccgctatt attcagaaaa
    29941 tgtgtcacct tagtgatggt ccagtcctgc tcgtctatga cgcgcttaaa gcctgacact
    30001 ctgaccggtg tttccgtgta aatatctgca cgaccggtag ccaggatgat ggagaactcc
    30061 gcaacgcccc gttgcagttt gtcccacttc gcctgagcag cacgcatggc ctgtgctttc
    30121 gtggcatata ccgtggtcag ggcaaaaacg ttgtcagcct caccggccat gtattcacct
    30181 tcgcgcgctt ccggtacttt tggcgctttc ttctgcctga ccggtttcgc tttcgggtgc
    30241 tccagtgcgc gcaggtgttt ctctttcttt ttgcgtttca gttttacctt ctgcttttgc
    30301 ggtttcgggt ctttggtgtg taaccacttt gccgttacac cggtataggc tccacggtca
    30361 gcaatcgcaa aatgatggcg gtcgccgtcg ctgcgggtta tggtgacctg cgggattttt
    30421 ttaccgctgg ccgtcacccc ctgccccgct ttgagaaaca acagttttcc catttttacc
    30481 gagacctcac cgccgttgcg ttcagcaagg cgggtcagga attttgcatc agactcctgc
    30541 gactggtcga tgtgcgggat tttaattccg gccagtgacg gcgcgacact ggcttccagc
    30601 ctgttacggg aggctattgc ctcaacaatc gcaccgagcg tggtgtcatg ccaggagtct
    30661 tcacggcggg aattgagcgt cccgcgaaaa tctgcactcc gggcgcggat ggtgaccaca
    30721 tccggcgcgc cccggtgttc aacctcatca acggtaaatt tccctttgca taccagggca
    30781 aaacctttcc agccgatata caccgtcagg acagcgccac gaatcggcag cccgacctgc
    30841 ccgtcggcat cgttcagttc aatatcaagc tggtcagcct caaagccccg gttatccgtc
    30901 agagtcatgc tcatcagacg gtcgctgata ttgccggtaa tatccctgct gtcgagcatc
    30961 agcatgtaat ccggcgtcag cgtactgcct gcatcaaatg tcagtgcatc cagcattatc
    31021 ccgcccccgt catacctgtg aatttagtcg ccatactgcc agccttaccg atgagcgatt
    31081 ccgcctgttt accgatatcg ccataaagcg cggccagtgg ttcgtcaacg cgggtgagcg
    31141 acagcgtaaa atcaattttt cggggtgtgc cgtctgcaaa gaaaatactc cctgtttcac
    31201 tcaccctgct gatgacatac atgccgtaaa tcatgccggt gccatccagc aacggccacg
    31261 cccgaccttc ctctgccatc agcctgagcg tggtcatcgt cagcttgccg ccggtcagtt
    31321 cgggataaag cacaccggcc agcgtcatgt tttcctcgcc aacgccgaga aactggtagg
    31381 catcccgttt accgatacgg gaatttgacg gccagcgata atctgattca cgctgcatgg
    31441 tctggtgtgg cagcgtctgg cgcataaaaa caaacatacc taacgcgagc atcatttttc
    31501 gtcacctcct taaccgtcat gcatcatgct ggcacgggcg cgcgcacgtt tatcccgctc
    31561 gtatttttcg agcgcatcct gtaactggcg gtcaagctgt gtccccggcg cagtaccacc
    31621 cgtcaggctg atgtgatatt cgtttttact ctggtccaca taagagcggc cagccggtgc
    31681 cgtgaccggc tgataagcct gatagcctgc ataagagctg gtcgccggaa tataaccacc
    31741 ggtgccatac gtggcggcat gagttctggc ggcggtctgg tcaagtgtgt ctgactcttt
    31801 gttgataaca ccgagttttt ccagtaccca gtcaatacca ctgcgcagtt tgttgaacgc
    31861 attaagcggc agcatcaacg cgtcagccag tgcctgcccg aacatgacgc ccgtgtcacg
    31921 gcaacggttc agggtgtcct gggtggcttt gaccggggca atcagttttt taaaccactg
    31981 ccacgcggcc tgtaactttt cacccagcca gtcaaacagc ggcttaagtg gcgtgaacag
    32041 ttcccccacc ggcgcaaatg cagctttcag cccttcaacc acaccgccaa agaatgcgct
    32101 gacaggctcc cagtatttac ggataagcaa cgccccggcg acaattgcag ccaccacggc
    32161 cacaaccggc cagctaatcg ccccgatggc cgtcataaca gcactgccaa ccgtcgtgaa
    32221 gattgccccc attgcgcctg ctgccgcgat gatggcatta atgccggtga taaccggcca
    32281 ggctacaaga ccaattgcac cgatgatgcc aataagcgcc agtgcgccac cgacaataag
    32341 gccgatggtt gacgccagtg atttgttttt ctggatccag ccgtcgagtt ttaacacata
    32401 ctttgtggcc gtctgcgtga gattacgcag tgcgccttcc tgttggtcaa acaggtcagt
    32461 ccccaccgcc tcataagcgg actgaaactc cttaaagtca ccgccgaggt tgtcctgcat
    32521 gatatttacc agctctgcgg tcttcccgtc tgaggcttta aacgcagcgg tcagtttgtc
    32581 cagctttccg gttgaggcgg cagtcatcag cacggcggcg gctgaactgg cctcctcccc
    32641 gaaaatggtt ttcatgtatt cagcctgctg ggcagtaccg agccggtttt tctcaaaact
    32701 ggcctgcatt tctttcagaa tggtaaatat tggtcgggta ttccccttgc tgtctgaggt
    32761 tttcactcca agctctttga gtgcatccca tgcttttccc gtcggtgcct gcaggcgact
    32821 taacacggca cggcttcccg tccccgccat tgagcctgtg atttttgcat catgcagcgc
    32881 cccgaccatt gcggcggttt cttcaatgct gacaccggca ttttttgcca caggtgcggc
    32941 ataggtcagc gcatcgctca taccgtcaaa atcggcggcg gttttgttca tcgtcatgga
    33001 gagaacatcc ccgatatgag caaccttatc gtttgaaagc tgaaaggcag atttcatccc
    33061 catcagcagg gcggcgtttt cttccatcgt gcgacggttc gccagcgcca tattcagcgt
    33121 gaccggcgtt gccgcctgaa tggcatcaac atccccaccc gctttcgcga tgataatctg
    33181 tgcaccggcc gcatcatccg ccgaggcggc ggtattgtcg ccgagctggc gcgcctgctt
    33241 gcgtagcgcg gccatttcgg cggagtcttt tgccacaccg agcacggcct gtaattctga
    33301 gtttttctgc gcaaattcat aaccgggcat cagcagctta acaccggcca tcgttcccgc
    33361 cgccgcaatc cccacaccgg cagcgcccac tgaggccata tttccggcca gctcctttcc
    33421 tgcctgatag cgctgtttta ctgcgttaag ttttgcctgt tgcgcactga cacgcgccag
    33481 cgcgtcacgc tgccggttaa gctgtgcggt ggtttcactg atacggtttt tcagccccag
    33541 ctcatcatgt gcaagattgc gggtattaat tcccacagcg gccagttccc gctgctggcg
    33601 tttaacggaa tccgtcaggc ggttatattt cgcctgtaag tcctccgccg cacgctttgc
    33661 ggattccagc actttcgcct gagcacgggt cggacgttcg gtgtttttaa actgtgtggc
    33721 aagggcttcg gcctcctgcc gtgccttttc aagtgcatga ccagtcacgg cgagctgtgc
    33781 gctggtcttg cggaatccct caatacggga tgcctgcccg ttcagctcgc gcagtgattt
    33841 ttgtgtttcc cggatatccc ccgacagcga cttactcgct gtgcggatgg atttaaacgg
    33901 gcgggatgcc tggtcaacag ccctgagcaa tacctgtaat tttacattgt tactcattcg
    33961 tgtttccgct tcgcctgagc gccttttcgc gccatgtgat gagttcggtc aggctcatgg
    34021 gatacagttc tgatggcggc cagtgaaata ccactgccac atccgccatc aggtcatcga
    34081 ccgagagatt tttcggaaac gtcactgcac cgagttcggc gacaaaaaac cgaccacctt
    34141 accggccagc gccacaaggt caggcagttc cagcgcggcg acctcctgtt cggtcagcat
    34201 cggtgccgtc atgcgcggca gcactttaat cagtgcatcg acttcggagt tcgcgaccgc
    34261 agccagactg acaccgcgca gcgtcccggc attgggtttc atcagcgtga cctgttcgat
    34321 aacctgctca ccacgcttga ccggattgtc cagggtaatc acattttctt tgttcatggt
    34381 tttctcactt ctgaatcagg gttaaccggt cagcctggct gaccggatga aaatcacagg
    34441 ccgatattgc ggcggtgttg ctccagccgg tcgacgccgt tcaccttctc aatcatgttg
    34501 atggtgtcga tttcgaccag ctccttaccg tccatcgtca gccggaaata ggtgcagacc
    34561 acggagattt tcgactcggt gtcttctccc tgtttaccct cgccggtgtc gatttctttc
    34621 tgacggccac gcatgaccac ctcgacggcc accgtttcgc cggtatcgtc gcgctggtaa
    34681 gagcctgcaa aacgaatcgg tacggcatcc acaccggttg cggcgtaaag ctcccagata
    34741 accgaatccg ggaagccacc gagcgaccac tccattgaca gcgcatcgtc atcaaggccg
    34801 aggtctaccg gtgcgctgcc attcatcccc gcaccgcgat agttttcgag cttacgggtc
    34861 agttttggca gcgtgacgga cttcgcgacg ccctgatagc tgtagccgtt cagaaagacg
    34921 ttcattaact tgagtttgcg cggcattgcc atcggtcagg ctccttaatt gctgttaacc
    34981 gaggtgacca gatttgccag gtatttatcg gtgatacgct ggcgcagggt caggttttca
    35041 agaggaggta ccggtgtata gtcgtagtcg atatacagtt ttccggcctt gagggtttcc
    35101 gcatcgctgg attcttcgct gaaccagcag gtcgcatcca cgatatagcc gtttgttttc
    35161 agctcacgga atttggcatt gatgccgtca acgatgtcgc gaatcagcgt tgcggtgatg
    35221 ggcttgtcca ccgcccacat gtgcgcctca gccatcgtgt cggccagcac ctgcgcggtg
    35281 cgggtgtagt tttcaaagag gaacagcggg tcatcagagc aggtacggtt accccagaag
    35341 cggaaaccgt cgcggcgaat cagcgtagtg acgcctgact cgttaagcag gtcagcatcg
    35401 gtgccggact cctgcaaatc ccagaataca gatgcgctga tgccgttaac accgtttacc
    35461 ccgacgttgg acagcgtttt atgccagccc tgctcctggt cgattttagc gcgcagaccc
    35521 agcgcacggg cggtggcata cgcggtggcg gtggtgctgc tgaccgtatc ccatgcgagg
    35581 aaatccggcc agatgaccat cagctcacgc tggctgaaat tctggcggta ggctttcacc
    35641 tcggaaatgg ttttacagcc ccatgcgctg atatacccga aagcgcgcag cttctgacag
    35701 actgatgcca gtgcaacagc cacctctttg gtgtccagtc ccggcacgcc gagaatacgc
    35761 ggtttaacac cggttaccga ctctgccgcc agcagggctt tcagtccggt atactgaccg
    35821 ttttcgtcag tggtgccgat gatattggaa acggtctgcg cgagtttcgt ttcttcgtcg
    35881 tcgccggtgc cgtcttcaac acgcacgaca acggtaaccg gttttgactg gtcagcgatg
    35941 gcctgcaacg atgccgccag cgtgcctttt ttaccggcct ttgcaattgc gctctgcaca
    36001 ttggtaatca gcacaggttt attgaggggg aaggtttccg catccgcatc gctggccgtg
    36061 cagaccatgc cgacaatggc ggtggatacg gtggaaatga cgcgggtgcc gtcgttaatc
    36121 tccagcacct gcacgccgtg atgataatca ctcatccgtt taactccgtg gttaatgggt
    36181 gagtgatatt ttcagttgtg ccggagatgt caggctattt gtcccggttg gctaagggat
    36241 gacacaattt attctttgtc gctgatgagg ggaatttttt atagagcgtg gacaggccaa
    36301 tatcaaaaat cagcgccaca cgttgacgag attcccccgc agccagcaac cgtccgacct
    36361 gctcccactc actcgtggtg agtttcggtc gtctgccacc aatacgacct ttggctctgg
    36421 ccgcttccag tccggcgcgt gtccgctcga caatgagttc tcgctccatt tctgccaggg
    36481 cccccatcac atgaaagaaa aagcgcccca tcggcgtgct ggtatcaata gcatccgtca
    36541 ggctgcgaaa attaacgccg cgttcgcgca gttcctcaac cagaatgacc agatgccgca
    36601 tactacgccc cagcctatcc agcttccaga ctaccagagt gtcacctgct gataatgttc
    36661 tgagcagttt tttcagtccc ggtcggtcgg acttcgtgcc gctgatttta tcctcaaaaa
    36721 tcagctcaca tcctgcacac tccagcgcat tacgctgcaa ttccgtgttc tggtcatttg
    36781 ttgacacgcg tacatagcca ataagcatga gcatctccct gaataaaaac cggagatgat
    36841 gccagttacc cgttatttct gcat
//
LOCUS       DBFIFM010000020        36190 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.020, whole genome shotgun sequence.
ACCESSION   DBFIFM010000020 DBFIFM010000000
VERSION     DBFIFM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.020"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            246..1619
                     /gene="aroP"
                     /locus_tag="N7C74_000935"
     CDS             246..1619
                     /gene="aroP"
                     /locus_tag="N7C74_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HGU2430859.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVTVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAVVLVIMLMTPGMAISVYLIPVWLVVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1662..2516)
                     /gene="ampE"
                     /locus_tag="N7C74_000936"
     CDS             complement(1662..2516)
                     /gene="ampE"
                     /locus_tag="N7C74_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HGU2430860.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFRTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2513..3064)
                     /gene="ampD"
                     /locus_tag="N7C74_000937"
     CDS             complement(2513..3064)
                     /gene="ampD"
                     /locus_tag="N7C74_000937"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HGU2430861.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3152..4045
                     /gene="nadC"
                     /locus_tag="N7C74_000938"
     CDS             3152..4045
                     /gene="nadC"
                     /locus_tag="N7C74_000938"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HGU2430862.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4248..4688
                     /gene="ppdD"
                     /locus_tag="N7C74_000939"
     CDS             4248..4688
                     /gene="ppdD"
                     /locus_tag="N7C74_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HGU2430863.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            4698..6083
                     /gene="gspE"
                     /locus_tag="N7C74_000940"
     CDS             4698..6083
                     /gene="gspE"
                     /locus_tag="N7C74_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HGU2430864.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLAGFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6073..7275
                     /gene="hofC"
                     /locus_tag="N7C74_000941"
     CDS             6073..7275
                     /gene="hofC"
                     /locus_tag="N7C74_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HGU2430865.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQRAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGIMLTIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNIAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7310..8353)
                     /gene="guaC"
                     /locus_tag="N7C74_000942"
     CDS             complement(7310..8353)
                     /gene="guaC"
                     /locus_tag="N7C74_000942"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HGU2430866.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIIS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8509..8553)
                     /locus_tag="N7C74_000943"
     CDS             complement(8509..8553)
                     /locus_tag="N7C74_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2430867.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8578..9198
                     /gene="coaE"
                     /locus_tag="N7C74_000944"
     CDS             8578..9198
                     /gene="coaE"
                     /locus_tag="N7C74_000944"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HGU2430868.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9198..9941
                     /gene="zapD"
                     /locus_tag="N7C74_000945"
     CDS             9198..9941
                     /gene="zapD"
                     /locus_tag="N7C74_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HGU2430869.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDCQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9951..10148
                     /gene="yacG"
                     /locus_tag="N7C74_000946"
     CDS             9951..10148
                     /gene="yacG"
                     /locus_tag="N7C74_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HGU2430870.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10239..10637)
                     /gene="mutT"
                     /locus_tag="N7C74_000947"
     CDS             complement(10239..10637)
                     /gene="mutT"
                     /locus_tag="N7C74_000947"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HGU2430871.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     KWMSLVGLNADDFPPANEPVIAKLKRVYVG"
     gene            complement(10697..13402)
                     /gene="secA"
                     /locus_tag="N7C74_000948"
     CDS             complement(10697..13402)
                     /gene="secA"
                     /locus_tag="N7C74_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HGU2430872.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13464..13976)
                     /gene="secM"
                     /locus_tag="N7C74_000949"
     CDS             complement(13464..13976)
                     /gene="secM"
                     /locus_tag="N7C74_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HGU2430873.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14207..15124)
                     /gene="lpxC"
                     /locus_tag="N7C74_000950"
     CDS             complement(14207..15124)
                     /gene="lpxC"
                     /locus_tag="N7C74_000950"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HGU2430874.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15225..16376)
                     /gene="ftsZ"
                     /locus_tag="N7C74_000951"
     CDS             complement(15225..16376)
                     /gene="ftsZ"
                     /locus_tag="N7C74_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HGU2430875.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16437..17699)
                     /gene="ftsA"
                     /locus_tag="N7C74_000952"
     CDS             complement(16437..17699)
                     /gene="ftsA"
                     /locus_tag="N7C74_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HGU2430876.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17696..18526)
                     /gene="ftsQ"
                     /locus_tag="N7C74_000953"
     CDS             complement(17696..18526)
                     /gene="ftsQ"
                     /locus_tag="N7C74_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HGU2430877.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18528..19448)
                     /gene="ddlB"
                     /locus_tag="N7C74_000954"
     CDS             complement(18528..19448)
                     /gene="ddlB"
                     /locus_tag="N7C74_000954"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGU2430878.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRVEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSVRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19441..20916)
                     /gene="murC"
                     /locus_tag="N7C74_000955"
     CDS             complement(19441..20916)
                     /gene="murC"
                     /locus_tag="N7C74_000955"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HGU2430879.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(20970..22037)
                     /gene="murG"
                     /locus_tag="N7C74_000956"
     CDS             complement(20970..22037)
                     /gene="murG"
                     /locus_tag="N7C74_000956"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HGU2430880.1"
                     /translation="MSAQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22034..23278)
                     /gene="ftsW"
                     /locus_tag="N7C74_000957"
     CDS             complement(22034..23278)
                     /gene="ftsW"
                     /locus_tag="N7C74_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HGU2430881.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23278..24594)
                     /gene="murD"
                     /locus_tag="N7C74_000958"
     CDS             complement(23278..24594)
                     /gene="murD"
                     /locus_tag="N7C74_000958"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HGU2430882.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGVQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24597..25679)
                     /gene="mraY"
                     /locus_tag="N7C74_000959"
     CDS             complement(24597..25679)
                     /gene="mraY"
                     /locus_tag="N7C74_000959"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HGU2430883.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25673..27031)
                     /gene="murF"
                     /locus_tag="N7C74_000960"
     CDS             complement(25673..27031)
                     /gene="murF"
                     /locus_tag="N7C74_000960"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HGU2430884.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIVWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFAEKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27028..28515)
                     /gene="murE"
                     /locus_tag="N7C74_000961"
     CDS             complement(27028..28515)
                     /gene="murE"
                     /locus_tag="N7C74_000961"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HGU2430885.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQDATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEDRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28502..30268)
                     /gene="ftsI"
                     /locus_tag="N7C74_000962"
     CDS             complement(28502..30268)
                     /gene="ftsI"
                     /locus_tag="N7C74_000962"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HGU2430886.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30284..30649)
                     /gene="ftsL"
                     /locus_tag="N7C74_000963"
     CDS             complement(30284..30649)
                     /gene="ftsL"
                     /locus_tag="N7C74_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HGU2430887.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30646..31587)
                     /gene="rsmH"
                     /locus_tag="N7C74_000964"
     CDS             complement(30646..31587)
                     /gene="rsmH"
                     /locus_tag="N7C74_000964"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HGU2430888.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31589..32047)
                     /gene="mraZ"
                     /locus_tag="N7C74_000965"
     CDS             complement(31589..32047)
                     /gene="mraZ"
                     /locus_tag="N7C74_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HGU2430889.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32649..33653)
                     /gene="cra"
                     /locus_tag="N7C74_000966"
     CDS             complement(32649..33653)
                     /gene="cra"
                     /locus_tag="N7C74_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HGU2430890.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33735..33821)
                     /locus_tag="N7C74_000967"
     CDS             complement(33735..33821)
                     /locus_tag="N7C74_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HGU2430891.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33833..34324)
                     /gene="ilvN"
                     /locus_tag="N7C74_000968"
     CDS             complement(33833..34324)
                     /gene="ilvN"
                     /locus_tag="N7C74_000968"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGU2430892.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLYKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34327..36051)
                     /gene="ilvI"
                     /locus_tag="N7C74_000969"
     CDS             complement(34327..36051)
                     /gene="ilvI"
                     /locus_tag="N7C74_000969"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HGU2430893.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIG
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGGAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(35993..>36190)
                     /locus_tag="N7C74_000970"
     CDS             complement(35993..>36190)
                     /locus_tag="N7C74_000970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2430894.1"
                     /translation="DFIVSFHLSSCLFLLPGVYVSGCQLLKQDRTVNVFYTFFPSNSE
                     AGHGDVVWSRDGRPIAYRSGR"
BASE COUNT         8664 a        10144 c         8890 g         8492 t
ORIGIN      
        1 cattctgcat attgagaggg gttgaggctg agctttacaa acggtttctt tttaagcaac
       61 tcatcttcaa ccatgcataa agcgggtgca ttcgctgccg cataccatta ttcttgatct
      121 gacggaagtc tttttgtaac tattcaaact tctttgatgt aaacaaatta atacaacaaa
      181 cggaattgca aacttacaca cgcatcactg cgtagatcaa aaaaacaatc accgcacgag
      241 gtttcatgat ggaaggtcaa cagcacggcg agcagctaaa gcgcggcctt aaaaaccgcc
      301 atattcagct tatcgcgctg ggtggcgcga tagggacagg gttattcctg ggtagcgcct
      361 ccgtaataca gtccgcaggg ccagggatta tcctgggtta cgccattgct ggttttatcg
      421 cctttctgat catgcgtcag ctgggtgaaa tggtggtcga agaacctgtc gcaggctcct
      481 ttagccactt tgcttataaa tactggggca gctttgctgg cttcgcttct ggctggaact
      541 actgggtact gtacgtttta gttgccatgg cagagctgac tgctgtgggt aaatacattc
      601 agttctggta tccggaaatc ccaacctggg tttctgccgc cgtgttcttt gtggtgatta
      661 acgccatcaa cctgaccaac gtaacagtgt ttggtgagat ggagttctgg tttgccatta
      721 tcaaagttat tgcggtagta gcgatgatca tcttcggcgg ctggctgctg ttcagtggta
      781 acggcggtcc gcaggcaagc gttagcaacc tgtgggatca gggcggtttc ctgccgcacg
      841 gcttcaccgg gctggtgatg atgatggcga ttatcatgtt ctcgttcggt ggtctggaac
      901 tggtggggat caccgcagca gaagctgata acccggagca aagtatcccg aaagcaacta
      961 accaggttat ctaccgcatc ctgattttct atattggttc gttagccgtt ctgctctcac
     1021 tgatgccgtg gacccgcgtt accgccgata ccagtccgtt tgtgctgatc ttccacgagt
     1081 taggcgatac ctttgtggcg aatgcgctga acatcgtggt actgactgcg gcgctctccg
     1141 tgtacaacag ctgcgtatat tgcaacagcc gtatgctgtt tggtctggca caacagggta
     1201 acgcgccaaa agcgctggcg tctgtcgata aacgcggcgt accggttaac accattctgg
     1261 tgtctgcgct ggttacagca ttgtgcgtat tgattaacta tcttgctccg gaatccgcat
     1321 ttggcctgtt aatggcactg gtggtatccg cactggtgat caactgggcg atgatcagtc
     1381 tggcgcatat gaagttccgt cgcgccaagc aggaacaagg cgtggtaact cgcttccctg
     1441 ctctgcttta tccgctgggt aactggatct gcctgctgtt tatggcggtg gtactggtga
     1501 ttatgctgat gaccccagga atggcgattt cggtatacct gatcccggta tggctggtgg
     1561 tgttaggtat cggctatctg tttaaagaga aaaccgcaaa agccgtaaaa gcacattaat
     1621 ctctctacgc cctcacccgt acagggtgag ggcaataatc tttacaccaa cgccccgtaa
     1681 atcgtcagta gcgcaatcac caccacgacc acgaacgagg ttttcttcgc cattgaaacc
     1741 gctgccttcg gcgtctccac cttatcgaca tgcggttcac gcgccagaga gaactgcgcc
     1801 agacgcgtta atacctgata ttgcgaagta cggaaatcac ccagcgaagc aaaccaggcc
     1861 ggtaacgctt tctcaccatg accgatcaag gcatatacca cacctgcaag acgaaccggc
     1921 acccaatcca gtacatgaag cactgcatca atgccggact gtaaacgatg atgcggcgtc
     1981 tggtatcgcg ccagccagta ttgccatgca cgcaaaaacg cataccccat cagcgtaacg
     2041 ggtccccagg ttccccccac aatcagccag aacagcggtg caagataaaa acgaaagtta
     2101 atccacagca atgcattttg cagctcacgc aaatactcac gttcgtcgca gcctgccggg
     2161 acgccgtgaa tcatggtgag ttcgccagcc atcgtggcac gggcatggct atcattacgt
     2221 gaagcagctg tcagataagc atgataatga agacgaactt tacctgcgcc aatacacagc
     2281 aaaccaatca gcagccacac cagcagcgtg ggaacgttga acaatactcc ctgcaatgcg
     2341 cgtaacagta aaaaagtcac gcccatcgca ataatggtca tgcctaacgt gcgcccgaga
     2401 gaaaaatgtt tcacccgccg aaagaacgct tcaagacgat gatcaagctg ccagtgctcg
     2461 cccaacttaa acaggcgctc gaaaattaac accagtaagg ttgtaaatag cgtcatgttg
     2521 tctccttgct gaccagcgca cgaaaccgtg cccaatcaaa tgcaggaccg ggatcggttt
     2581 tccgatccgg cgcaatatca caatggcccg tcatgttatt agcgatatcc ggatagcaat
     2641 caatcagtgc ccgcgtaacc gccgcaagct gttgatactg cgcatcggta tacgccagcg
     2701 tatcggtgcc ttcaagctca atcccaatag aaaaatcatt gcagcgttcg cgcccctgat
     2761 actgagagac tcccgcatgc catgcacgtt tatcgaaagg aacatactgg actatttcac
     2821 catcacggcg aatcaaacag tgagcggaga cgcgcaaatg ggcgatctca gcaaagaaag
     2881 gatgtgcctg cggatcaata gttccagtga ataatgcgtc gatccacgga ccgccaaact
     2941 cgcctggcgg caggctaata ttgtgcacca ccagcagggt gggtgtttcg tcatccgggc
     3001 ggcaatcgta atgtggtgag ggaacgcggc gcgcgccaac cagccacccc tgttctaaca
     3061 acatgcagga tctccttata tgtggtgcta atacccggtt cagagtagca tgtttctacc
     3121 ttatgattcg ttagctatct ggagttttaa catgccgcct cgccgctata accctgacac
     3181 ccgacgtgac gagctgctgg aacgcattaa tctcgatatc cccggcgcgg tggcccaggc
     3241 gctgcgggaa gatttaggcg gaacagtcga tgccaacaat gacattacgg caaaactttt
     3301 accggaaaat tctcgctctc atgccacggt gatcacccgc gagaatggcg tattttgcgg
     3361 caaacgctgg gttgaagagg tgtttattca actggcaggc gacgatgtca ccataatctg
     3421 gcatgtggat gacggcgatg tcatcaacgc caatcaaccc ttgttcgaac ttgaaggccc
     3481 atcccgcgtg ctgttaacgg gcgaacgcac tgcgcttaat tttgtgcaaa ccctttcagg
     3541 agttgccagt aaggtacgcc actatgtcga attactggaa ggcaccaaca cgcagttgtt
     3601 ggatacgcgt aaaaccttac ccggtctgcg ttcagcgctg aaatacgcgg tactttgcgg
     3661 cggcggagcg aatcaccgtc tggggctttc tgatgccttc ctgatcaaag aaaaccatat
     3721 tattgcctcc ggctcagtgc gccaggcggt cgaaaaagcg tcctggctgc acccggatgc
     3781 gccagtagaa gtcgaagtag agaatctgga agaacttgat gaagccctga aagcaggagc
     3841 cgatatcatc atgctggata acttcgaaac agaacagatg cgcgaagccg tcaaacgcac
     3901 caacggcaag gcgctactgg aagtgtctgg caacgtcact gacaaaacac tgcgcgaatt
     3961 tgccgaaacg ggcgtggact ttatctccgt cggtgcgcta actaagcacg tacaggcact
     4021 cgacctttca atgcgttttc gctagttgct gacagattta tccgctccat agtgggcgga
     4081 taatctcccc ttccccaacc ttcttcgtaa cgcctcgcaa atttcttgtc tttcgctgca
     4141 acgaaataaa aaacctcgga agcagctttc ccgtttcgtt ttttgcccct cgcctgtgct
     4201 gatggcgttt gccaaagtag caccaaccaa atcaaggagc gaaacagatg gacaagcaac
     4261 gcggttttac acttatcgaa ctgatggtgg ttattggcat cattgccatt ttaagcgcca
     4321 ttggtattcc cgcttatcaa aactacctgc gcaaagccgc actcaccgac atgctacaaa
     4381 cctttgtgcc ttaccgtacc gccgtagagt tgtgcgcgct ggaacatggt ggattagata
     4441 cctgcgacgg tggcagcaat ggcattccct cgcctaccac cacccgctat gtttcagcca
     4501 tgagtgtggc aaagggcgtg gtgtcgctga ccgggcaaga aagtctcaat gggctaagcg
     4561 tcgtcatgac gccaggttgg gataacgcaa acggcgtcac cggctgggcg cgcaactgca
     4621 atattcaaag tgacagcgca ttgcagcaag cctgcgaaga tgtcttccgc tttgatgacg
     4681 ccaactaagg agcggcaatg aatattccac agctcacggc cctgtgcctg cgttatcagg
     4741 gagtcttgct ggatgccagc gaagaagtgg ttcatgttgc ggtggtcgat gccccctcac
     4801 atgagttgct ggacgcattg catttcgcta ccaccaaacg tattgagatc acctgctgga
     4861 cgcgccaaca aatggaaggt cacgccagtc gcacacaaca gacattgccc gtagctgttc
     4921 aggagaagca tcagcccaaa gcagagttgc tgactcgaac gttacaatct gcgctggaac
     4981 aacgcgcgtc tgatattcat atcgaaccag cggacaatgc ctaccgcatc cgcttgcgta
     5041 tcgacggcgt attgcatcct ttaccggatg tttcaccgga tgccggagtc gcattaaccg
     5101 ccagattaaa agtgctggga aacctggata ttgcggaaca tcgcctgccg caggacgggc
     5161 aattcactgt cgaactggca ggaaacgccg tctcatttcg tattgcgacc ttaccatgtc
     5221 ggggtggtga aaaggtggta ttaaggttgt tacagcaggt gagccaggca ctggatgtta
     5281 acacgctggg aatgcagccg ttacaactgg cgggctttgc tcatgccttg caacaaccac
     5341 agggactggt gctggtaact ggccctaccg gcagcggcaa aacggtcacg ctttatagtg
     5401 ccctgcaaaa gctgaatacc gctgacatta atatttgtag cgtcgaagat ccggttgaga
     5461 tccccatagc cggactaaac cagacgcaaa tccatccgcg tgccggactc acctttcagg
     5521 gcgttttgcg tgcgttattg cgccaggatc ctgacgtcat catgatcgga gagatccgcg
     5581 atggcgaaac ggcagaaatt gccattaaag ccgcgcaaac cggtcacctg gtgttgtcta
     5641 ccctacacac taattccacc tgcgaaacgc tggtacgttt acagcaaatg ggggtcgccc
     5701 gctggatgct atcatcggcg cttacgctgg taatagccca gcgtctggta cgcaaacttt
     5761 gcccacattg tcgccggcag caaggggagc ccatccacat tccagtcaat gtatggccgt
     5821 cgccgctgcc ccactggcaa gcacccggtt gtgtacattg ctaccacggt ttttatggtc
     5881 gcactgcctt atttgaagtt ctgcccataa caccggtcat acgtcagctt atttccgcta
     5941 ataccgacgt tgaatcgctg gaaacgcacg cccgacaggc gggtatgcga acgctttttg
     6001 aaaacggctg cctggccgtg gagcaaggct taaccacctt tgaagagtta atccgcgtat
     6061 tggggatgcc gcatggcgag taagcaactc tggcgctggc atggcataac cggcgacggc
     6121 aatgcgcaag atgggatgct atgggcagag agccgtactt tactgcttat ggcactacag
     6181 caacagatgg ttaccccact aagcctgaag cgaattgcta tcaattctgc gcagtggcga
     6241 ggagataaaa gcgcggaagt cattcatcaa ctggcgacgc tacttaaagc cgggttaacg
     6301 ctttctgaag ggctggcact gctggcggaa cagcatccca gtaagcaatg gcaagcgttg
     6361 ctgcaatctc tggcacacga tctcgaacag ggcattgcct tttccaatgc cttattaccc
     6421 tggtcagagg tatttccgcc gctctatcag gcgatgatcc gcacgggtga actgaccggt
     6481 aagctggatg aatgctgctt tgaactggcg cgtcagcaaa gagcccagcg tcagttgacc
     6541 gacaaagtaa aatcagcgtt acgttatccc atcatcattt tagcgatggc aatcatggtg
     6601 gttgtggcaa tgctgcattt tgttctgccg gagtttgccg ctatctataa gaccttcaac
     6661 accccactac cggcactaac gcaggggatc atgacgctgg cagactttag tggcgaatgg
     6721 ggctggctgc tggtgttgtt cggcattatg ctgacgatag ccaataagtt gctgatgcgc
     6781 cgcccgacct ggcttatcgc gcggcagaaa ttgctgttac gcatcccgat tatggggtca
     6841 ctgatgcggg gacaaaaact cacgcagatc tttacgattc tggcgctgac tcaaagtgca
     6901 ggcattactt ttttacaggg cgtagagagc gtcagagaaa caatgcgctg cccgtactgg
     6961 gtgcaacttc tgacacaaat ccagcacgat atcagtaacg gtcatcccat ctggctggcg
     7021 ttaaaaaatg ccggtgagtt tagcccgctc tgtttgcaat tagtgagaac gggagaggcc
     7081 tccggctcgc tggatctcat gttagacaac atcgcccatc atcatcggga taacacaatg
     7141 gcgctggcgg ataacctagc agccttactg gaaccggcgt tgctgatcat aacgggagga
     7201 attatcggta cgctggtggt ggcgatgtat ctgccaattt tccatttagg cgatgcgatg
     7261 agtgggatgg gataacgctg gcgtgttgct ccacaccagc gttggaagat tacaggttgt
     7321 tgaagatgcg gttttcttgt tcctgcacac ggataaatgt ggtgcgcttg gtcagttctt
     7381 tcaggcgtga agccccaacg tatgtacaag ctgaacgcag gccgcccaaa atatcacgcg
     7441 cggtattttc aaccgggcct cgcagcggca gcttaacggt tttaccttct gctgcgcgat
     7501 attcagcaac gccgccaacg tgacgtttca tcgcagactc ggagctcatg ccgtagaaca
     7561 gcataaattt ctcgccgttc tcctcaacga tgcgaccgcc gctctcttcg tggcccgcca
     7621 gcatgccgcc aagcatgacg aaatcggcac cgccaccaaa ggctttcgcc acatcgcccg
     7681 gcgtggtgca gccaccatcg ctgatgatca ttccgcccag accgtgcgca gcatcggcac
     7741 attcgattac cgcagaaagt tgcggataac cgacgcctgt tttgacgcga gttgtacaaa
     7801 cagaacctgg gccaatgcca actttaacga tatcggcacc tgagaggata agctcctcac
     7861 acatttcacc agtcactacg ttaccagcac aaatggtttt ggtcggccac gcttcacgcg
     7921 ctttcgcaac gaactgcacg aagtgttcgg aataaccatt cgccacgtca atacaaacga
     7981 agtttaatgc cgggttcagg tcgagaatct gtttagtttt ttcgaaatcc gcatcagacg
     8041 taccggtaga aaccatcaca tgtttcagca catcagcgga agaattgttg ataaacgctt
     8101 gccactcttc gacagaatag tgtttatgca cagcagtcaa aatatcaaaa gaagccagcg
     8161 cagaggccat agaaaatgtg cctacggtgt ccatatttgc ggcgataatc ggcacgccgg
     8221 accagctctg acctgaatgt ttgaaggtga attgacgttc cagttcaaca tcggaacggc
     8281 ttttaagagt ggagcgttta gggcggatga gaacgtcttt aaaacctaac ttcagatctt
     8341 cttcaatacg catgtgcgga ttcctggggt taatggctat aagggtaaga gcgcaactcc
     8401 agtgacgcta tcatacgcac taatcaatgc ggcgcaagac tgcgaaattg tcttttttta
     8461 cgctacaatc ccataaattt acctggtaaa tagtgagcga acgtttcatc aatttcgctt
     8521 tcgtgcgctc atttttaacg ttttgataat catgatgaat tcccgggaat ataaattatg
     8581 aggtatatag ttgccttaac gggaggcatt ggcagtggca agagtaccgt tgccaatgcg
     8641 tttgctgatc tcggaattaa cgtcattgat gccgatatta ttgcgcgtca ggtggttgaa
     8701 ccaggtgcac ctgcgctaca tgccattgct gatcactttg gcgctaatat gattgctgct
     8761 gatggaacat tgcagcgccg ggccttgcgc gagcggatct tcgccaaccc ggaagagaaa
     8821 aactggctta acgccctgct gcatccgctg attcagcaag agacgcaaca ccagatccag
     8881 caagctactt ccccctatgt actgtgggtt gtgccattgc tggtagaaaa ctcactgtat
     8941 aaaaaagcga atcgagtgct ggtggtggat gtcagcccag aaacgcaact taagcgcacc
     9001 atgcagcgcg atgatgtaac tcgcgagcat gtcgaacaaa tccttgctgc tcaggcaacg
     9061 cgcgaagccc gccttgccgt ggcagatgac gtcattgata ataacggcgc accggatgct
     9121 atcgcatcgg atgttgcccg cctgcacgca cactatttgc agcttgcgtc gcagtttgtc
     9181 tcacaggaaa aaccgtaatg cagacccagg tcctttttga acatccacta aatgaaaaaa
     9241 tgcgtacatg gctgcgcatt gagtttttga ttcagcaact caccgttaat ttacccatcg
     9301 ttgaccacgc tggcgcgctg catttcttcc gtaatgttag tgaattactg gatgttttcg
     9361 agcgcggcga agtccgcact gagctgttga aagaacttga ccggcagcaa cgtaaactcc
     9421 agacctggat tggcgtgcct ggcgtggacc agagccgtat tgaagcatta attcagcagt
     9481 taaaagcggc ggggagcgta ttaatttccg cgccgcgtat cgggcaattt ctgcgtgaag
     9541 atcgtttgat tgctctggtg cgtcagcgac tgagcatccc aggcggctgt tgcagctttg
     9601 atttacctac attgcacatt tggctgcatc taccccaggc gcagcgcgac tgccaggtag
     9661 aaacctggat tgccagcctg aacccgctca cccaggcact taccatggtg ctggatttaa
     9721 ttcgccagtc ggcccccttc cgtaaacaaa ccagcctgaa tggtttttat caggataacg
     9781 gtggcgatgc cgacttgctg cgcctgaatc tgtcgctcga ttcacagctt tatccgcaaa
     9841 tttccggaca taagagccgt tttgccattc gttttatgcc gctggacact gaaaacggac
     9901 aggtaccgga acgtctggat ttcgaactgg cctgttgcta aggagtaaaa atgtcagaaa
     9961 ctattacggt gaattgccca acctgcggga aaacggtggt gtggggtgaa atcagcccgt
    10021 ttcggccatt ttgctccaaa cgttgtcagc tgatcgacct cggagaatgg gctgctgaag
    10081 aaaaacgaat ccccagcagt ggcgatcttt ccgaaagcga tgactggagc gaagagccaa
    10141 agcagtgaca tttgtctgat gccgcacgta ggcctgataa gacgcggaca gcgtcgcatc
    10201 aggcatcttg tgcaaatgtc ggatgcggcg tgaacgcctt atccgacata cacacgctta
    10261 agcttcgcaa ttaccggttc attggctggc ggaaaatcat cggcattaag accgactagc
    10321 gacatccact taccgggttg cccttcttta ccccacggct ccccttccca gctttcgacc
    10381 agccagaacc acagagttat atgcctgtcc gggaattcat attccagttt ttcaaatagc
    10441 gaaaaatgtt gtggggtaat cccgacttct tcctgaagtt cacgcaccac cgcctgttcc
    10501 ggcgtttcac ccatttcaat tttaccgccg ggaaactcca gtttattcgc catgtgcgca
    10561 tctgctgcgc gacgcgttat aaagatttca ttgttctcgt tgcgaataat acctaccgca
    10621 atttgcagct ttttcattgt cttaaaccta taaaaaaggc gcagaatcct gcgcctttta
    10681 cttcaacagt tagcttttat tgcaggcggc catggcactg cttgtatttt ttaccagaac
    10741 cgcacgggca aggatcgtta cgtcctactt tgcgctcacc ggtttgcgcc gccagtgcag
    10801 ctgcggctgc agagtcgtca tcctgatggc taagctgctg catttgcgct aaacgctcgg
    10861 cttccatacg acgctgttgt tccagctcct caacctcttc aggcatacgt acctgaactt
    10921 tgctcagcgt actgataact tcatatttca acgactccag catcgctgca aacatggaga
    10981 acgattcacg tttgtattcc tgcttcggat ctttctgtgc atagccacgc aggtggatac
    11041 cctgacgcag atagtccatc gctgccaggt gctctttcca cagggagtca agcgtttgca
    11101 gcatgacgcc tttctcgaag tgacgcatca tctcagcacc aaccacttct tctttacgct
    11161 gatacacttc gatggactgc gccagaatgc gctcacgcag cgtctcttca tgcagttctg
    11221 gttctttatc cagccactca gcaattggta aatcgaggtc gaaatcgttc ttcagacgtt
    11281 cctgcagccc cggaatatcc cacatttctt ccagcgactg tggcggaatg taggcatcaa
    11341 tggtcgcttt gaacacatct tcacgaatgc tgttaatggt ttcgctcaca tcgctgacat
    11401 ccagcaattc gttacgctgg gagtaaatgg cgcgacgctg atcgttagcc acgtcatcat
    11461 attccagcag ttgcttacga atgtcgaagt tacgactttc aactttacgc tgggcgttgg
    11521 caatcgcttt agtcacccac gggtgttcaa tggcttcgcc tggcttcata cccagtttac
    11581 gcatcatgcc ggatactcgg tcggaagcaa aaatacgcat cagcgcatct tccatcgaca
    11641 ggtagaaacg ggaagaacca gcatccccct gacgaccaga acgaccgcgc aactggttat
    11701 cgatacgacg ggattcgtga cgctcggtac cgatgatatg caggccacct gctgccagta
    11761 ccgcatcgtg acgtacctgc cagtcggctt taattttttc aatttgctct gcggtcggat
    11821 tttccagcgc ggcaacttct gcctgccagc taccaccgag cacaatatct gtaccacgac
    11881 ccgccatgtt ggtcgcgata gtcaccgcag ccggataacc tgcctgagca acaatcgccg
    11941 cttcgttggc gtggaatttg gcgttcagga cattgtgctt aataccggct ttggtcagtt
    12001 cgtttgacac cagctccgat ttttcgatgg agatagtacc caccagcacc ggctggcctt
    12061 tcgcagtacg ttctttgata tcttcaatga tcgcctgaat tttttccgct tcagtcatgt
    12121 agaccaggtc cggcagatct ttacgaatca ttggacggtt ggtcggaaca acgacggtat
    12181 ccagtttgta gatggagcta aattcgaaag cttcggtatc agcagtaccg gtcatccccg
    12241 ccagtttttc atacagacgg aagtagttct ggaaggtgat cgaagccagc gtttggtttt
    12301 cgttctggat ctgcacacct tctttcgctt ccacagcctg gtgcagacca tcggaccagc
    12361 gacggccctg catggtacga ccggtgtgtt cgtcaacgat gataacttca ccatctttaa
    12421 cgatgtagtc gacgtcacgg gtaaacagcg catgagcgcg cagcgccgcc gttacgtggt
    12481 gcatcagcat gatgttggcc ggagagtaca gagactcccc ttcatccatg atgccctctt
    12541 tcaccagcag ttcttcaatc agcaccagac cacgttcggt caggttcacc tggcgagatt
    12601 tttcgtccac cgagaagtgg ccttcgccct ggaaggtttc ggagtcttct ttttcctgac
    12661 ggatcaggtg cggaataatt ttattcacgc gtttatacat ttccgagctg tcttctgccg
    12721 ggccggaaat gatcagcggt gtacgcgctt catcgatcag gatggagtcc acttcgtcca
    12781 ccagcgcata gtgcagttta cgctgtacac gttcttcagg gctgaacgcc atattgtcgc
    12841 gcaggtagtc aaagccgtat tcgttgttcg taccgtaagt gatgtcagct gcgtaagctt
    12901 cgcgctttgc cggtgctggc atgcccggca ggttgatacc gacagtcagg ccaaggaatt
    12961 caaacagcgg acggttgttt tcggcgtcac gttgcgccag gtagtcgttg acggtaacta
    13021 catgcacgcc tttaccggtt agtgcgttca gataagcagg cagcgttgcg gtcagggttt
    13081 taccttcacc ggtacgcatt tcggcgatgc agcgttcgtt aagaaccata ccgccgagta
    13141 actgaacgtc gaagtgacgc ataccaaaga cgcgcttact tgcctcacgt accacggcga
    13201 aagcttccgg gatcagattt tccagcactt cgcctttttc cagacgtgca cgaaactctg
    13261 cggttttccc tttcagttct tcgtcggaga gtttttccat ctccggttcc atggcattga
    13321 tgatgttgac cactttgcgc atccggcgca gggtgcgatc gttacgacta ccgaaaactt
    13381 tagttaataa tttgattagc ataataaaat ctcaaacgcc ccgcgttgcg gagttaataa
    13441 aatgaagtaa aggtttattg ttgttagctg aggcgttgag ggccagcacg gatgccttgc
    13501 gcctggctta tccagacggg cgtgctgaat tttgcttgtg gggtaaaatg cgcataatca
    13561 atgcgataac ccttttcaga cggcgtgcct tcctgggtca gcagcgcgct gagcgtatcc
    13621 agtaatgcaa gatgttgcgc ctgaagaggc aaagattctt cagcaacggg cagcgtttgc
    13681 ggtgccattg cgaaggaaag atgacggatt accgtgcgaa tggcatgttg atgccagtaa
    13741 tctacggaat agttcgaatt cgggcggcgc gtgttcgctt ccagcaaggc caattgacca
    13801 aagttaactt tggctgaagg ctcgtggttg cgggttgtcg cttttgcggg cgcgtttggt
    13861 tcggcggcgt tgctgagcgc aggcaaacct aaactcgccg caaccatccc taataagaga
    13921 tgcggccaga agtagcgttt accaaactgt cgccagcgcg tcagtattcc actcacgtta
    13981 ttgccatccc gatcgtattc aaacgtattt agcgcacaaa tcttatcatt aaagcctgaa
    14041 gtccacagca cgaatgaaga taagttgtcc gtaaaagtgc ttaagaaaac agcgttcgca
    14101 ccgtttttta tagctcttat aaaattcaaa aaaaaacgac tggttcacct ggccggagag
    14161 agtgccagat ttgccagtcg aattttatac gacagtataa atgtcgttat gccagtacgg
    14221 ctgaaggcgc tttgaaggcc aacggcagtt ctgcgtcgtc ctggaaggtc acatattccc
    14281 aggcttcctg tttcgccagg acagcctgca gcagtttgtt attcagtgca tgaccggatt
    14341 tataagcggt aaatgcacca ataatattgt gaccacacat gaacaagtca ccgatcgcat
    14401 cgagcatttt gtgacgcaca aattcgtctt caaaacgcag gccgtcttcg ttcagtacgc
    14461 gataatcgtc aacaacgatg gcacaatcga agctgccgcc caggcacaaa ccacgggact
    14521 gcagatattc gatatcacgc atgaaaccga acgtacgcgc acggctgatc tggcgcataa
    14581 acgcatcagc ggagaagttc atcgcatagc gctggttgct ggaatcaata gccggatggt
    14641 taaaatcgat ggtgaaatcc agcgaaaaac cattgtacgg cttaaattca gcccacttat
    14701 cgccatcttc gacacgaaca gtctctttga tgcgaacaaa ttttttggcg cagttcaact
    14761 cgtcgatacc ggcgtcaagc agcaggtata caaacggagc ggcgctgccg tccatgatcg
    14821 ggatttccgg cgcgttaact tcgataacaa tgttatcgat gcccaagccc gcgagagcag
    14881 cattgaggtg ctctacggtt gaaatccgta catcatgctc gttgaccaga cacgtacaga
    14941 gcatggtatc acgcacagat ttggcatcgg ccgggaaatc taccggtgga ttcaagtcgg
    15001 tgcgacgata gatgaccccg gtgttggccg gcgcagggcg taacgtcagg gtgactttct
    15061 tgccggtatg taaaccgaca cccgtcgcct gaacgatacg tttaagtgtc ctttgtttga
    15121 tcatcgtatt atctcgccaa attacctatc caaccgaagt gtactataca ttcggcgggc
    15181 cagtttagca caaagagcct cgaaacccaa attccagtca attcttaatc agcttgctta
    15241 cgcaggaatg ctgggatatc cagataatcc ggctctttcg cagtttgcgg cgcattgtca
    15301 ttcacgactt tagcaaccgg cttctgctcc tgggtcagcg gagccatccc atgctgctgg
    15361 tagcgatcca tcactggctg ctgaacctgc ttattggtca ccagagtgat ttcaggacgt
    15421 ttgtccatgc cgatacctgt cgcaacaacg gttacgcgca gctcgtcatt catatccggg
    15481 tcaagagaag taccgataac cacagtcgcg ttgtcggaag caaatgcacg gatggtgtta
    15541 cctaccgttt cgaactcatc cagacgcagg tcgaagcccg ctgtgatgtt aaccagcacg
    15601 ccgcgtgcgc cagacaggtc gatatcttcc agcagcggag aagagatagc catttcagca
    15661 gcttcttccg cacggtcttc accgctcgcc acgccagaac ccatcattgc gtagcccatt
    15721 tcagacatta cggtgcgtac gtctgcaaag tccacgttca tcaaacccgg acgagtaatc
    15781 agttcggcga tgccttgcac agcgcctttg agtacatcgt tcgctgcgcc aaacgcatcc
    15841 agcagggaga taccgcggcc cagaactttc agcagtttgt cgttcgggat agtgatcaga
    15901 gagtccacat gcttggacag ttcagtgatc ccctgctccg cgaatgccat acgcttcttg
    15961 ccttcaaagt tgaagggctt agtgacgaca gcaacggtca ggatacccaa atcttttgcc
    16021 acttcagcga cgactggtgc tgcacctgta ccggtaccac cacccatacc cgcagcaata
    16081 aagaccatgt ctgcaccttc cagcgccgca cgcagtgcat cgcgatcctc atctgccgca
    16141 ttgcggccaa cttctggatt agcgccagcg cccagtcctt tggtgatacc gctaccgatt
    16201 tgaatcgtct gtccaaccgc tgttttacgc agcgcttgtg catcggtatt taccgcgaag
    16261 aattcaacac cttcaatgcg ctcgcgcacc atgtgttcaa cagcattacc gccgccgccg
    16321 ccgacgccga tgactttaat caccgcgtca ttggtaagtt ccattggttc aaacatagtt
    16381 tctctccgat ttgtgcctgt cgcctgaggc cgtaatcatc gtcggcctca taaaaattaa
    16441 aactcttttc gcagccaact attgagtcgc ttgatccacg agccaactga tgctgtaaca
    16501 cgtttttcta cttcagcttc accgttaaga tgtgactctt tcccatagtg aagcaatccc
    16561 accgccgtcg aataatacgg ctcctgagca taatccgtta aaccggtaat gttcagcggc
    16621 gcgccgatac gcacttgcgt atgaaacacg cgctgagcac aggctgcaag accttcgatc
    16681 tgcgctgcgc caccggttaa tacaatgcct gccgccaggt gatgtttaac cccttgttgg
    16741 cgaagctttt cctgcaactg caatatctct tcgttgacca ggttgagcag ctcggtatag
    16801 cgcggctcga tcacctctgc cagggtctga cgttgcagac tccgcggcgg acgaccgcct
    16861 acgctcggca cttccacgct ctcatctttt ccaacgatgg aacccagcgc acaaccgtgg
    16921 cgaactttaa tcgcttcggc gtcgcttggc ggcgtgccaa aggcgtaagc gatatcactg
    16981 gtcacgacat tgccagcata aggaattacc ttagtgtggc gcaatgcccc accggtataa
    17041 acggcgatat ccattgtacc accaccgata tcgacgacgc agacacccag ttcacgttca
    17101 tcttccgtca ataccgaata acttgatgcc agaccggcaa atatcagttg gtcaactttc
    17161 agcccacaac gttcaaccgc tttgacgatg tttttcgcca tatcgttgtg gcatgtgatc
    17221 aggtgcactt ttgcctgcat ccgcacgccc gaaagcccta ccggattctt gatcccttcc
    17281 tgatagtcaa tcgcatactc ttgcgggatc acatgcagca cacgatgctc atcgcgtaca
    17341 cgcaccgatt tcgcggtatg gacgacgttt tccacatctt cttgcgttac ttcttcttca
    17401 gaaataggca ccataccaat ttcattctgg cagctgatgt gcttaccaga aagcgccaga
    17461 tataccgaag agatctgaca atctgccatc aattctgcct ggtcaatggc acgttgtacg
    17521 cacttgacca cggattcgag gtcgttcacc ccgcctttat ccataccacg cgacgggcag
    17581 ctgcccacgc caatgatatt gaccataccg tcgggcagaa cttcccctac taaagcggca
    17641 accttcgcgg taccaatctc cagtcctact accagttttc tgtccgtcgc cttgatcatt
    17701 gttgttctgc ctgtgcctga ttttgttgct gagtagattc ctctggcggc aagggcgccc
    17761 agcctactgc cgctccagag tcataacgca aatcaacgta gctaatccgt ttgccatcgg
    17821 tttgcgcctg ctgctgtaga accggataaa gttctacaaa gcgagccaaa cgtttcatcg
    17881 tatcgccccg gccaagattg agcttaatat cgttattcag cgtcaactgc caggaacgcc
    17941 gcgcggtcat cgccgcttcc ttcagagtaa atctgtcctt tgccagcatc tgccccattt
    18001 cgcgatagcc ctgcaacact tcattggcgc tgccttccgg gccatacagc attggaagca
    18061 cctgcttgct ggtgcgatct ggcggcacgc tgaaggtatt tccttccgcg tctaccatgt
    18121 gttgatcatt ccaccgcgca atcggcacat attcaaccag atgaatcttc aattcatcag
    18181 gccactgctt tctgacgctc acctgcttaa tccacggcag acgttgttct atttgcgtct
    18241 ggatgatgtt gacatcctgg gtcataaagg tacccggctc acccaatgcc aggatcgact
    18301 gccggatatc gtcattacgc gtgtaatggc gttcaccggt caacaccagc tttgagagcg
    18361 gcaggcgttg cgcatcttcc atccagccca acacgaccca gccgctcacc aacactgtcg
    18421 ttaaaacggt cagcaggaaa aggatccccg ccagacgcgt tccattattg cggcgagaag
    18481 aaacctcttc ttcgctgttt cgcgtgttca gagcagcctg cgacatatta gtccgccagt
    18541 tccagaattc gtactaccaa ctgcgagaag ctcatacctg cctgacgtgc cgccatcggc
    18601 accaggctgt ggctggtcat acccggtgag gtattggctt ccagcagata aaactgtcca
    18661 tcgctgtcca gcataacgtc aatacgtccc catcctttgc aacctaacgt cgtccatgct
    18721 ttcagcacta atgcctgcaa attggcctct tgtgacgctt ccagacctgc ggggcagaaa
    18781 tactgtgtct catcagagag atacttcgcc tcataatcat agaaggttcc ggacggttga
    18841 atacgtactg acggtaaaat ttcttcaccg agtatcgcaa ccgtgaactc cggcccactt
    18901 agccattttt caatcaatac ttcttcatcg tgctgaaatg ccaatcttaa tgcatcttgt
    18961 agagcatttt ctgctactac ttttgacatt cccacactgg aaccttcgcg gctcggctta
    19021 acgataaccg gcaaacccag agcagaaatt tctgctaact gcttatcact caggcctttt
    19081 tcaaactcta cgcgggttaa cgctacccac ggcgcgaccg gtaaaccggc accttgccac
    19141 agaagtttgc tgcgtagttt atccattgaa agcgcagatg ccatcactcc gctgccggta
    19201 taaggcaagc ccatcagctc gagcatcccc tgcagcgtac catcttcacc gccgcggccg
    19261 tgtagcgcga taaacacttt ctgaaagccc atcgacttca gttgcgtcac gtcaacttct
    19321 ttcgggtcga caggatatgc gtcaataccg ccttcacgca gcccggctaa caccgctgcg
    19381 ccagaattca gagaaacttc ccgctcagcg gaagtcccgc ccaacaggac cgcgatttta
    19441 tcagtcatgt tgttcttcct ccggagtttg cggcttcagt ttgatttcag ctaaagaacg
    19501 ggcaattttt ccgatattac cagccccctg aacgagaatc aggtcgttac cggttaatac
    19561 cggtgccagc atctcggcta cctgcgcagg gtccggcacc agaatgggat ctattttccc
    19621 acgtccacga attgtgcgac acagcgaacg gctgtccgca cccggaattg gcgcttcgcc
    19681 agccggatac acttccagca tcaacagggt atcaacctgc gtcagcacat tggcgaaatc
    19741 atcatacagg tcgcgtgtac gggtaaaacg gtgcggctga aacagcatta ccaggttttt
    19801 atccggccag cctgcgcgcg ccgctttaat ggtggcgtcc acttccgtcg ggtggtggcc
    19861 gtagtcatcg accagcattg ccgtaccgct tttaccattc actggctcca gcgggaattc
    19921 accgaggaaa tcaaaacggc gaccagtccc ctggaagctc tccagcgcac gcaaaatagc
    19981 ctcgtcgtca atgccctctt ccgtagcaac cgcaaccgca gctgcggcgt tcagcgcgtt
    20041 atgacggcct ggcgcattca gggtgacgcg catcggctct ttgtcctggc gcagcagcgt
    20101 aaagtgcccc tgcgggccaa tctgctgata atcttctaca cgcacgtcgg catcttcgct
    20161 gaagccgtaa gttgtggtct gacgtcccac tcgcggtaac aattcgcgga tcaccggatc
    20221 atcaacacac atcaccgcac gaccgtaaaa cggcaggttg tgcagaaaat taataaaagt
    20281 ctgttttaaa ttctcaaagt cgccctggta ggtatccatg tggtcggctt cgatattggt
    20341 gacaatcgcc accatcggtt gcagatgcag gaacgatgca tcactctcat ctgcttcggc
    20401 aatcaggtac cgaccatgcc ccaaacgcgc atgaaccccc gccgctttta ccagcccgcc
    20461 gttaacgaag gttgggtcga gccccgcttc tgcgtagatg ctggagacca tcgcggtagt
    20521 tgtcgttttg ccgtgcgttc ctgcaatggc gatgccatga cgaaaacgca ttaactcagc
    20581 cagcatttcg gcacgacgga tcaccggaat acgcgcttca tgagctgcga caatttccgg
    20641 gttatcggca gaaatggcgc tggaaacaac gaccacgctg gcatcacgta cgttttccgg
    20701 gcgatggttg aaataaatcg tcgctcccag attcattaac tgctgcgtga ccggatttgg
    20761 cgctaaatcg gaaccactga tctgataacc ttcattggcc agaacttcgg caataccgcc
    20821 cataccggca ccaccgatgc cgacaaaatg tatgtgccga acgcgacgca tttcgggcac
    20881 gatggaacgc agttttgcca attgttgtgt attcattctt tacgccatta acttcttaaa
    20941 ttcatacgat gcaaaaggca tcgctacaat tacgcccggg cagcccgact cacttcattt
    21001 gccactcgct cggtggcatc cggaatggat gcagcgcggg cgcgttctgc catggttaat
    21061 aaggtttctc gcgaccaccc ggccagggtg ttggcgacag catccacgct aagctgtggc
    21121 tgctcgataa ttttggctgc gcccgctttt tccagcggta gcgcattcca gtattgctgg
    21181 cggtctttat gttgaaacgg cacaaacaac gccggtagtc ctgccgcggc gatttcactc
    21241 accgttaaag caccggagcg gcaaacgacg acatccgccc acgcatacgc cgccgccata
    21301 tcatcaataa attcagtcac tttatgctgc ggttgccccg cttcggcata cgcctgttca
    21361 acggattgtt gcgaaccttt gccgctctga tgccagatag tgactgaatc acccagtttc
    21421 gcagcaacct gcggcattgt ctggttaaga atgcgtgcgc cctgggaacc acccactacc
    21481 agcacacgaa ccggaccttc acgtccagcc aaacgttgct gcggcaacgg tagcgccaac
    21541 acatcggtac gcaccgggtt acccaccact tccgcattgg ggaaagcacc tggaaacgcc
    21601 tgcatcactt tggtggcaat cttcgccagc catttattgg ttaagcccgc aataccgttt
    21661 tgttcatgaa gtacaaccgg aatgcctaac gaccacgcgg ccagaccacc aggacctgat
    21721 acgtagccgc ccataccgag caccacgtca ggtttgtacg ctttcataat cgcccgcgcc
    21781 tgacgccagg cattgaagat acgcagcggc gcagctatca gtgcttttat accttttcca
    21841 cgcagaccag agatacgaat gaaatcaatt tcgatgccat gttttggcac taagtccgct
    21901 tccatacggt cggcagtccc cagccagcga acttgccaac cctgagccat tagatggtgc
    21961 gcaaccgcca gtcccgggaa tacatgtcca ccggttccgc ctgccatcac cattaatcgc
    22021 tttccttgag cactcatcgt gaacctcgta caaacgcctg cgctttctcc agacgcgttt
    22081 cataatcaat acgcaacagc atcatgatgg ctgtcgacat aatcagtaag ctcgaaccac
    22141 cgtaactgat cagcggcaat gtcagacctt tggtcggtaa catccccgcc gccgcgccta
    22201 cgttaaccag cgcctggaag ctaaaccaga tgccaataga acaggcgaga aaaccggaaa
    22261 aacggtggtc aatttctaat gctttacggc caatcgacat cgcgcgaaaa gcgacgaaga
    22321 ataccattaa aagtgccagc accacaccga cataccccag ttcttcgccg ataatggcga
    22381 aaataaagtc agtgtgcgct tccggcagat actccagttt ttgtacagag ttacctaaac
    22441 cttgtcccca cagttcgccg cgaccaaacg ccatcagcga ttgcgttaac tgatagccgc
    22501 tgccaaaggg atcttcccac gggttccaga atgcggtaac acggcggata cggtacggtt
    22561 cggcgagtat cagcaacaca accgctgaaa tgcccatacc gataatggca atgaactgcc
    22621 acaatttcgc tcccgccagg aacaacatcg ccagcgtagt cacaaacaac accaccaccg
    22681 taccaaggtc tggctgtgcc agcagtaaca ctgccaacac cagaatcacg cccatcggtt
    22741 tcaggaagcc gcgcaggtta ttacgtactt cgtcaccttt acgcaccaga tagttggcga
    22801 tatagcaaaa cagcgacagt tttgtcagct cagcaggctg gatacgcagc aaaccgagat
    22861 cgatccaacg cgatgcccct ttaaccgagc tccccactac cagcacgatc atcagcagga
    22921 tgatagatcc gagcagcatc gtggcactgt agcgttgcca gaactccatc ggcagacgca
    22981 gcgtaatgat cgccagaata aacgccaaaa tcagatagac accatcacgc ttcgcgaaga
    23041 agaacggatc gttggttaag cgttgcccta tgggcattga cgccgaggtc accatgataa
    23101 agccaatcgc cgcgaggccg aaggtcagcc acagtaaggt gcgatcgtac atgatcaggc
    23161 tgtcggtatc tttttcccgc gagcccatca cccagccctt tagcgccgtg gagatccaga
    23221 ccaggatact gaatcctggc aggcgcggca ttttcaggcg agggagagat aaacgcatca
    23281 acctaactcc ttcgccagac gggcaaactc attgcctcgt tgttcaaagt tcttgaactg
    23341 atcaaggctg gcacaggccg gggagagtag aaccatatcg cccggctgaa cacgcggagc
    23401 cagcaagcgc atcgcctgtt ccatagtttc ggtttgttct gccacttccg ggcgtagcgc
    23461 cgccagctgc acgccgtcac gaccgaaaca atacagacgt acgttatcgc cattcaggta
    23521 acgcgccagt gggctaaagt ccgccgattt accatcgccg cccagcaaca aatgcagcgt
    23581 gccgtctacg tgcaggccat tcagcgccgc ttccgtactg ccgacgttgg tcgctttcga
    23641 atcgttaatc caacgtacgc cgttatgctc cagcacaact tcaaagcgat gcggcagacc
    23701 agtgaatgtg gttaacgctt tcaggctgct ggcacgcggt aaccctgcgg catctgccag
    23761 cgccagcgcc gccagcgcat tggtgtagtt atgctgcccg gaaagtttca tttctttcac
    23821 gttcagcact ttctcgcctt tcacccgcag ccaggtttcg ccctgctgat gattcaggtg
    23881 atagtcaccc atgttgacgc caaagctgac gcagcgttca tccgcaccgc gaatcggcat
    23941 tgttaaggca tcatcagcat tgacgacaca aactttcgca ttttcgtaaa tacgcaattt
    24001 tgccgcacgg tattgctgta aaccaaacgg atagcgatcc atatgatctt cagtcacgtt
    24061 cagaatggtc gctgctaccg cctgtaagct ggaggtggtt tccagctgga agctcgacag
    24121 ttccagtacg tacagttcgc actcatcatc cagcagcatc aacgcgggca ggccaatatt
    24181 gccacccaca ccaacgttaa cccccgccgc tttcgccatt tcacccacta gcgtggtgac
    24241 cgtgcttttg ccgttagaac cggtaatcgc cacaatcggt gcttgtgctt cgcgacagaa
    24301 cagctcgata tcgccaacga tttcgattcc ggcatcagcg gcagcgctta aggatggatg
    24361 cgccagtgca ataccgggac tggcgacaat cagatctgcc gccatcagcc attcatcatt
    24421 cagactgccc gtgtggcgtt ctacggcttc gggtaattta tccaggccag gcggtgtcat
    24481 acgcgtatcc ataacgcgcg gcgtcacacc gcgagcgagg aaaaagtcca cgcaggaaag
    24541 cccggtgagg cccaggccga taatgacaac atttttaccc tggtaatcag ccatgattaa
    24601 cgtaccttca gcgttgccag accaatcagg accagcatca gcgaaataat ccagaaacgc
    24661 acaatgacgc gcggttccgg ccagcctttc agttcatagt ggtgatgaat cggtgccatg
    24721 cggaaaatac gttgtccgcg cagtttaaag gagccgacct gcaggatgac ggaaagcgtt
    24781 tctaccacga acacgccccc cataatcacc agcaggaatt cctgacgcag cagtacggcg
    24841 ataatgccta acgcaccacc taacgccagc gaacctacat cgcccataaa gacctgcgcc
    24901 ggataggtgt taaaccacag gaagcccagc cctgccccga ctatcgcggt acagacaata
    24961 accagttccc cggcgtgtcg cagatatggt atgtgtaaat agctggcaaa gttcatgttg
    25021 ccggtcgccc acgccaccag cgcaaaacca ccggcgacaa atacggtcgg cataattgcc
    25081 agaccgtcaa gaccatcggt caggtttacc gcgttgccag tacccacaat gacgaagtaa
    25141 gccagcagaa tgtagaacag ccccagttgc ggcatcacat ctttaaagaa tgggaccacc
    25201 agctgcgttg cgggcgtgtc tttgccaaca aggtacagcg cgaaggcgac acccagcgca
    25261 atgaccgaca tccagaaata cttccaacga gcgatcaacc ctttggtgtc tttacgcact
    25321 actttgcgat aatcatcaac aaagccaata acaccgtaac ctaccagcac caccaacacg
    25381 caccagacgt acggattgga cgggtaagcc cacagcagta cggagatcac aatcgccgtc
    25441 aggatcataa tcccgcccat ggtcggcgta ccgcgcttgc tgaagtgtga ttcaggaccg
    25501 tcgttacgca ccacctgacc aaaggaaagt ttttgcaaat gagcaatcat acgcgggccc
    25561 atccacaatg agatgaacag cgcggtcagc aggctgacga tggcgcgaaa cgtcagatag
    25621 gaaaagacgt taaagccgga ataatatttg accaaatgtt cggccagcca aactaacatg
    25681 tcccattctc ctgtaaagcg cgtactacct cttccatggc ggcactacgt gaacccttaa
    25741 ctaaaatcgt aattacctgt tgctcagcaa tcagtgattt aagacgcgta attaacgcag
    25801 ttttctcagc aaaatgttcg ccaacgccgc tggcggtgct gatagcatgg ctttgtttac
    25861 ccacgcttaa cacgcggtca ataccagcag cttttgccgc ctcgcccacc tgtacatggc
    25921 aggcttcgct ttcagcgccc agttccgcca tatcgcccac caccagcacg cggtagcccg
    25981 gcatttcagc cagtacctga acagcggcgg tcattgaacc gacattggcg ttgtaggagt
    26041 cgtcgagcag caactggttt tctgccagtt gaatggggaa caaacggcct ggaacagctt
    26101 tcagatttgc cagccccgct ttgatagcat caagcgttgc gcccacggac atggagagcg
    26161 cagcggctgc cagcgcattc gcaatattgt gacgccccgg caacggcagc agaacatcca
    26221 cgctaccggt tggggtttgc agggtaaatt ccgtaccgtg cgaagtcaca tggatattgg
    26281 tggcggtgaa atcgctgttg gcggcattag gtgagaaacg ccacactttg cgtgagccaa
    26341 ttacgctctg ccagttcagc cagtcgttgt tgtcggcgtt cataatggcg ataccgtttt
    26401 ccggcaggcc gctaaagatt tcacctttcg ctttcgcgac acccgcaagc gagccaaaac
    26461 cttccagatg cgccgctgcc aggttgttga ccagcgcagc ttccgggcga gtcagactca
    26521 cagtccagac aatttcgccc tgatggttcg cgccaagttc aataactgcg taatcgtatt
    26581 ccggcgttaa gcgcaacagc gtcatcggta caccgatgtc gttgttgaga ttgcctgccg
    26641 tataaagcgt gttgccgcac tggcttaaaa tcgccgccgt catctcttta acggaggttt
    26701 tgccggagga acctgtcaga gcaaccacgc gcgccggaac ttgctggcga acccatgcag
    26761 ccagttcacc aaacgccaga cgcgtatcct tgacgattaa ctgcggcagg tcgatatcca
    26821 gcggacggct aaccagtagt gcgcctgcgc cgccagcttt cgcctggtcg gcaaaatcat
    26881 gagcatcgaa acgttcgcct ttcagggcaa caaacaggca gcccggcgtc agttttcggg
    26941 tatcagtggt tacagcatca agggtgatat ctgcaccttg cagttcaccg ttgagaatgt
    27001 cggtaagttg gctaagggtt acgctaatca tgcaatcacc cccagcagac gcgccaccgt
    27061 gacgcgatcg gagtagtcca gacgctgatt gccaacaatc tggtaatctt catggccttt
    27121 gcccgcgacc agcaccacat cgttctcttt agcctgcata acggcgcaag tcaccgcttc
    27181 agcacggtct tccatcactt tggcatgtcc ggcatctaac attcccgcca gaatatcgtt
    27241 gatgatggca cgcggctctt cggtacgcgg attatcgtcc gtcaccaccg ccacatcagc
    27301 aaactcttcg gcaattgcgc ccatcagtgg acgcttacct ttatcgcgat cgccaccaca
    27361 gccaaagaca caccacagct tgcccgcaca gtgcagacgc gccgcctgta aggctttttc
    27421 cagtgcatcc ggcgtatgcg cgtaatccac caccaccgtc ggtttgcctg gcgcagtgaa
    27481 cacttccata cgtccgcaaa ccggttgcag acgagcagcg gttttcagca gatcagccag
    27541 tggatagccc agcgccagca gcgtcgccag cgcgagcagc aggttgctga cgttaaaagc
    27601 gcccatcaga tggctttcaa tttcgccatc gccccaactt gagctaaagc gaatagtcgc
    27661 accgctgtcg tgatagttca cttcggtcgc tttcaaccag cgtccgtgac agttcggatt
    27721 gatatgatct tccattgata ccgcaactgc atccggcagt tttgccagcc agcggcggcc
    27781 tacttcatcg tcggcattaa taatcgcctg accgcaatga tgttcagaat aaagcagcca
    27841 tttcgcagct tcgtagtgtt ccatatcacc atgataatca aggtgatcgc ggcttaagtt
    27901 agtaaagacc gacgccgcaa atttcaatgc cgccacacgg tgctgtacca gcccgtggga
    27961 ggaaacttcc attgcgcaaa aagtcgcgtc ctgatccacc agccccgcca gctcatgctg
    28021 aacatcaact gccgaaccgg tggtattttc tgtcgggatc actttcccca gcagaccgtt
    28081 accaacggtg cccattaccg cgctggtttc gccaagcagt tggctccact gcgccagcag
    28141 ctgggtcgtc gtggttttgc cgttggtgcc cgttacgccc acgagacgta aattgtcaga
    28201 gggttcatgg taaaagcggc ccgccagtgc agataaacgc tcgttgagct ggctgagata
    28261 gatgaccggt acgccgtgca tttcacggat ttcaccgtcg gtcgcctcac ctttcgcctc
    28321 tgcaataatg gcagccacac cttgcgctat cgcctgcggg atatatcgac gcccgtccgc
    28381 ctgatgacct actacagcta caaagagatc gcccgccgca gccacacggc tgtcgagtgt
    28441 catctctcgc agtgctcgcg aaggtgcgtc tggcacccac ggagcaagaa ggtcgcgcaa
    28501 attacgatct gccacctgtc ccctcgcctt gattaatcac aaattcattt ttatcgcccg
    28561 ttgtcagcgc atccggctcg atgttcatgg ttcgcaatac gccgcccatg atggcaccaa
    28621 agaccggcgc ggaaacggcg ccgccgtagt atttacccgc ctgcggatcg ttgataacaa
    28681 caaccagcgc gaagcgcggc tgactcgcag gcgcaacacc tgcggtgtaa gcaatatatt
    28741 tattgatgta gcgaccgtcc ggcccgacct ttttcgcggt accggtttta atggcaatac
    28801 gatagccttt aatcgccgcc ttcacgccgc cgccacccgg tagcgccacg ctttccatca
    28861 tatgcaccac agtgcggaca atagattccg ggaagacacg ttcaccggga accggggggt
    28921 caactttggt aatcgacagt gggcgataaa tgccgtagct gccgatagtt gcgtagactc
    28981 gcgctaactg taacggagtt accattagcc cgtagccgaa agagaaggtg gccctctcta
    29041 tgtcagacca ccgttgtttt tgaggatata agccactgcg ttctccgacc aaccccaaat
    29101 tggtcgcttt tcccagtcca aaacgtgagt aagtatctac taacgctgag gacggcatcg
    29161 ctaacgccag cttggaaaca ccgacgttac tcgacttctg taatactccg gtcagggtta
    29221 attcgctgta gcgtgccacg tctttgattt cgtggccgtt aattcgataa ggaatggtat
    29281 tgagtaccga gttttcccgc accacgccac gttgcaacgc ggtcatcacc accatcggtt
    29341 taaccgttga acccggttca aacacgtcgg tgatggtacg gttacgcatc gcctctttcg
    29401 gcgtgccgct cagattgtta gggttgtatg acgggctgtt agccatcgcc agcacttcac
    29461 cggtgttgac atccaccagc acggcgctac cggactcagc cttgttaaag gccaccgcgt
    29521 tgttcagttc gcgataaacc agcgcctgca ggcgttcatc aatactcagc gccaggttgt
    29581 gcgcagcctg gctgtcagta gaagaaatat cttcaattac gcgaccatag cggtctttac
    29641 gcacaatgcg ctcacccggc tgcccggtaa gccatttatc gaaactcttc tcaacgccct
    29701 caataccttg actatcgacg ttagtaaagc cgatgaggtg agcagtcact tcgccggatg
    29761 gataataacg gcgagattct tcacgcagat gaattcccgg cagtttcagt tttttgatgt
    29821 agtccgccat gtcagggttt acctgacgcg ccagataaat aaagcgccct ttcgggttgg
    29881 cgttaatgcg ggcagaaagc tgatccagcg gaatattgag cgcgttagcc agcgccttcc
    29941 agcggtcacc gacgctgata ccgccagcgt catgcacttc tttcgggtca gcccaaatcg
    30001 cttttaccgg cacgctcacc gctaacgggc gaccagaacg gtcagtaatc atgccgcggg
    30061 aggtggaaac ttgctgaacg cgaagagaac gcatgtcgcc ctctttcacc agcatatccg
    30121 gggagataac ttgtaaccac gctacgcgtc cgagcagaaa agccagcgcc aggagaatac
    30181 agccgcataa caacgcaaaa cgccaactga taaagttggc atgttcttcc tgacgttttg
    30241 gtttctgcgt tttcgccgct gctttcatgc gtcgcgttta tccttatttt tgcactacga
    30301 tattttcttg tgacggatca acatgctgca tttgcagctt ttccgtggcg atcctttcca
    30361 cccggctatg gtcgccgagc gcattctctt caaggatcag gttgcgccat tcaatgtcta
    30421 aagcatctcg ctccagcacc agttgttcgc gctgagcggt cagtaaacgg gtatggtgcg
    30481 ccgtggttac cacggtcacc gccgtcaaaa taatgcaaat gaacaggcag agtggcagct
    30541 tcccaaatcg caaaagatcg tcaccgataa caccaggcaa tgcatggcgc tcgtggcttc
    30601 ccatcgatcc tttaactttg cttagagctt ctgtcactct gctgatcatg cgttcgtcct
    30661 ctctgcaata cgcagaactg aactacgggc acgaggattc tcagccacct cttcttcgcc
    30721 cggcattaat ttgcctagtg ctcgcagctg acggccaccc agttttttga gctgctcttc
    30781 agtcatcggt aaccctgccg gaacttgcgg accgcggctg ttttcacgca taaaacgttt
    30841 cacaatacgg tcttccagcg agtggaagct gatgatcgaa agccgcccac ccggggccag
    30901 cacgttgagc gagcttttta gcgcctgctc tatctcctcc agttcactgt ttacccaaat
    30961 gcgcaccgcc tggaaggtac gggtcgcggg atgtttaaac ttatctttca ccggcgttgc
    31021 agcagccacg acttccgcca gttctttggt gcgggtcatc ggctgttcgc ggttacgctc
    31081 gacaatggct cgggcaatgc gtttggcaaa acgctcttca ccataggttt tcaataccca
    31141 ggcgatatcg gcttcttctg cggtttgtag ccattcagcg gctgactgcc cacgggttgg
    31201 gtccatacgc atgtccagcg gaccatcgcg cataaaggaa aagccgcgtt cagcatcatc
    31261 aagttgcggt gaagagacac caagatcgag gagaatgccg tcgatcttgc cgataagatc
    31321 gcgctcggca acgtattcgc ccagcgcgga aaaaggtccg tggatgatgg agaagcgcgg
    31381 atcatcaata gtcttcgcaa cggcgatagc ctgcgggtcg cgatcgatcg ccagcaaacg
    31441 cccctcttcg ccaagctgcg agaggatcag acgtgagtga ccaccgcgac caaaagtccc
    31501 atcaatgtag atgccatcag gacggatatt gaggccatta acggcttcat ccagcagcac
    31561 cgtagtatgt ttatagtttt ccatcatttt atagagacaa gtcctgcagt cgctccgata
    31621 agtctccggt agccaactgc tctgcgtcga tatcttcctt gacctgttga tgccaggttg
    31681 tttcatccca cagctcaaac ttgttgaact gtccaaccag catcacttct tttgtcagcc
    31741 cggcatgttg ccgcagtact ggcgcgatta acaatcgacc tgcgccatcc atctgacatt
    31801 cgctggcatg acctaacagt aggcgctgca cacggcgctc aaccgggttc atgctcgaca
    31861 gacgcgataa tttttgctcg ataatttccc attcaggcag ggggtaaagc agcaggcacg
    31921 ggtgatgaat gtcaatggtg caaaccattt gaccggcagc gttctcaagc agctgttccc
    31981 gataacgggt aggcactgat aagcgccctt tgctgtcgag attgactaac gttgctcccc
    32041 ggaacatgcc agcctcaccc cttattccca ctttgcccca caatttccca ctgaaaggag
    32101 tttacggagc ggaggaaaag cttgtcaagc cagtcacaag gcataccgag gtaaaaaacc
    32161 cgatgtttac ggctaatatc agcgttgttt attttacgtc caacgtgcga agcaaattaa
    32221 cgttatgaat agctgtaaga aaaaaaccct catcgtcatc accgttttaa aatgactcac
    32281 tattttccac agcaaatata aagtgtcagt ttgcgacgcg agcggcattt taggacatat
    32341 cttccccggt taacagtccc tgttgcgtct gtgtagcgct tgatgccagc gggtttgcgc
    32401 aaaagcaaag aaaagtgctt gttaaatcgg caattcatct ctgcatgtaa caaaataatg
    32461 caaaaacgcg ctgtcgatat cacttaatta ttgtgaattt cagctggtga gttaattaac
    32521 tggcgggttt aaaagcattt atcaataata gtcaggaata gtcttattta ctttaaggat
    32581 attgatgtcc agtcccgtac tctacgcgcc agagtgaaat tcacctggcg cgtatttttg
    32641 ttcgcagctt agctacggct gagcacaccg cggcgataga gattacgttt aatgcgcgtt
    32701 aaaccaggtt ttggcttacg cggttcgtcc aggcttgcca ggacaatctc cagcacacgc
    32761 tctgcgacat cgcggtgacg ttgagccact gccagcaccg ggcactgtaa gaagtcgagc
    32821 agttcgttat cgccaaaggt ggcaattgcc aggtcagaag gcagtttacc atcgcgacgt
    32881 agcgtgacat ccatcacccc ttgcaacaac gcaaacgacg ttgtgaacag cgcctgcggc
    32941 atcggatgcg tttccagcca tttttcgaat aactgggcag ccgcctcccg ctcatagctg
    33001 ttggcataca ggaaatgcac ttcgcgcgga tcatctttcc aggcagtacg gaaaccttgt
    33061 tcacgcagga agctgacaga aagctccggc agcgcgccaa gataaagcac cgtctcggcg
    33121 ggaaacttac gtaactcttc cgccagcatt tcggcatcat cctgatcggc accaaccacg
    33181 ctggtaaagt gttcacgatc gagggcgcgg tccagcgcga caatcgggaa cgggtcgtta
    33241 gcccagcgtt gataaaaagg atgctcagga ggcaacgacg tcgaaacaat aatggcatcg
    33301 acctgacgct gtaaaaggtg ctcaatgcag cgcatttcgt tgtctggctg atcttctgag
    33361 caggcaatca gcagttgata accccgttgc cgcgcctggc gttcaagata gttagcgatg
    33421 cgggtatagc tggtgttctc cagatcgggg atcacaagac caatagaacg tgtgcgtcca
    33481 gcacgaagcc cagctgccac ggcgttcggg tggtaattgt gctcacgcac cacagccatg
    33541 actttttcaa cggttttgtc actcacacgg tattgcttcg ctttgccgtt aataacatag
    33601 cttgcagtgg tccgcgacac tcccgccagc cgagcgattt catccagttt cacaattgcc
    33661 ccttgcgtaa aatgtaaaaa ccatagccat tgtactggta tgggttaaat tcattaacat
    33721 ctaagcgcag aaaattaact gcggcaactg cttttattcc aggttcccac tgatttcgca
    33781 aaaaaagccc aacgtgacat gttgggctgt aaattgcgca ttgagatcat tctcaacgca
    33841 ttattttatc gccgcgcgaa agtccgacca caccagagcg agcaacttcc acaattttcg
    33901 ccacatcgcg aatcgatgct aaaaatgcat caagcttatc gctggtgcct gctaattgaa
    33961 cggtataaag cgagggtgtg acatcaataa tttgcccacg gaatatttcc gtattacgtt
    34021 taacttcgtc acgcccgtaa ccgctggcct gaattttcac cagcatgatt tccctctcaa
    34081 catgcgcgcc ctgccccaac tcactcacgc gcaagacatc gaccagcttg tataattgct
    34141 tttcgatctg ctcaagcact ttttcatcgc ccacggtctg gatggtcata cgcgataatg
    34201 tcggatcgtc ggttggcgca acggtcaggc tttcaatgtt gtagccacgc tgggaaaaaa
    34261 ggccaatcac gcgggataac gcgcctgatt cattttcgag taagactgat aatatccggc
    34321 gcataatcag gttctctccg ttttgcttaa ccacatttca tccattccgc ccccgcgaat
    34381 ctgcatcggg tagacgtgct cactgccatc gacggtgaca tcaacaaata ccaggcgatt
    34441 attacgcacc tgttcaagcg cctcgctaag tttgctttcc agctcttgcg gatgagagat
    34501 ctggatcccg acgtgaccat aggcttccgc cagacggacg aagtctggta aagactgcat
    34561 ataagattgt gaatgacgac cggaatagat catgtcctgc cactgcttca ccatccccaa
    34621 atagcggtta ttaagattaa ccaccagcac cggcagttca tattgcaacg ctgtagacag
    34681 ttcctgaatg ttcatctgaa tactgccgtc gccagtgacg caaaccacgg tttcttctgg
    34741 cagcgccatt ttgacgccca gtgccgcagg taaaccaaag cccatagtgc cgaggccacc
    34801 ggagttgatc caacgacggg gtttgtcgaa tggataataa agcgcagcaa acatctggtg
    34861 ctgcccgaca tcggacgtaa cgtaagcgcc ccccttcgtc aaccgccaaa gagtctcgat
    34921 caccgcctgc ggtttaatct tttcactgtg agtgtcatat ttcaggcact gacgagcgcg
    34981 ccactgttca atctgctgcc accagtcgcg gatctcatcc agcggttgat gggcggattc
    35041 ttgcgacaag agttcaagca tttgttcgag gacctggcga gcatccccca caatcggaat
    35101 atccgcagtc acggttttag aaatggaagt aggatcaata tcgatatgca gaacagtggc
    35161 atttgggcag tactttgcca gattgttagt ggtgcgatcg tcaaagcgga caccgacagc
    35221 aaaaatcaca tccgcgttat gcatcgtcat attggcttcg taggtaccgt gcattcccag
    35281 catgcccagt gcctgacgat gcgttgccgg aaacgctccc agccccatca atgaggaaac
    35341 aacgggcaga tttaacgcct ccaccgtttc tttcagctgt tgatggcagc ccgccgtgat
    35401 tgccccaccg cctacgtaga caaccggttt ttttgccgcc accagcgttt gcagagcgcg
    35461 cttaatttgc cctttatgtc cggtagtagt gggattgtaa gaacgcatac tgaccgactc
    35521 cggccagaca tagggtaatt tgttcgccgg attaagaata tctttcggta aatcaacgac
    35581 cactggtcct gggcgaccac ttgccgccag ccagaaagcc tttttcagca cctgcggaat
    35641 gtcttccgtt tgcttaacca gaaaactgtg tttaaccacc ggtcgcgaaa tccccaccat
    35701 gtcgcactcc tgaaaggcat cgtaacctat caacgaggtc gctacctgcc cggaaaggac
    35761 aactaatgga atggaatcca tataagcggt ggcgatgccg gtaatcgcat tggtcgcccc
    35821 tggacccgac gttaccagca cgacgccgac ttccccggtc gcgcgcgcca gaccatcggc
    35881 catatgcact gccgcctgct catgacgaac taaaacatga ccgataccac ccacggtatg
    35941 caatgcatcg taaatatcaa ggactgcgcc tccgggataa ccgaatactt gtttaacgcc
    36001 ctgatcgata agcgatcgga cgaccatctc ggctccagac aacatctcca tggcctgcct
    36061 cactgtttga cggaaaaaat gtgtaaaaca cattaaccgt ccgatcttgc ttaagcaatt
    36121 ggcagccaga gacataaacg ccgggtaata agaataaaca ggaggaaagg tgaaaagaaa
    36181 cgataaaatc
//
LOCUS       DBFIFM010000021        35728 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.021, whole genome shotgun sequence.
ACCESSION   DBFIFM010000021 DBFIFM010000000
VERSION     DBFIFM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.021"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            45..1700
                     /gene="aslA"
                     /locus_tag="N7C74_000971"
     CDS             45..1700
                     /gene="aslA"
                     /locus_tag="N7C74_000971"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HGU2430895.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKEPQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIKQLPFSKDDVHAVRGGEQQAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDVFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLAAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(1868..3103)
                     /gene="aslB"
                     /locus_tag="N7C74_000972"
     CDS             complement(1868..3103)
                     /gene="aslB"
                     /locus_tag="N7C74_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HGU2430896.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETTAREGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVSK"
     gene            complement(3250..3326)
                     /locus_tag="N7C74_000973"
     tRNA            complement(3250..3326)
                     /locus_tag="N7C74_000973"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3290..3292),aa:Pro,seq:tgg)
     gene            complement(3369..3455)
                     /locus_tag="N7C74_000974"
     tRNA            complement(3369..3455)
                     /locus_tag="N7C74_000974"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3419..3421),aa:Leu,seq:cag)
     gene            complement(3476..3551)
                     /locus_tag="N7C74_000975"
     tRNA            complement(3476..3551)
                     /locus_tag="N7C74_000975"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3516..3518),aa:His,seq:gtg)
     gene            complement(3610..3686)
                     /locus_tag="N7C74_000976"
     tRNA            complement(3610..3686)
                     /locus_tag="N7C74_000976"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3650..3652),aa:Arg,seq:ccg)
     gene            complement(3789..5174)
                     /gene="yifK"
                     /locus_tag="N7C74_000977"
     CDS             complement(3789..5174)
                     /gene="yifK"
                     /locus_tag="N7C74_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU2430897.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(5365..6105)
                     /gene="wecG"
                     /locus_tag="N7C74_000978"
     CDS             complement(5365..6105)
                     /gene="wecG"
                     /locus_tag="N7C74_000978"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HGU2430898.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEENAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTTVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6108..7460)
                     /gene="wzyE"
                     /locus_tag="N7C74_000979"
     CDS             complement(6108..7460)
                     /gene="wzyE"
                     /locus_tag="N7C74_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HGU2430899.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7457..8536)
                     /gene="rffT"
                     /locus_tag="N7C74_000980"
                     /gene_synonym="wecF"
     CDS             complement(7457..8536)
                     /gene="rffT"
                     /locus_tag="N7C74_000980"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HGU2430900.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDASLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPHNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8533..9783)
                     /gene="wzxE"
                     /locus_tag="N7C74_000981"
     CDS             complement(8533..9783)
                     /gene="wzxE"
                     /locus_tag="N7C74_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HGU2430901.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISLGLFGNHDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAVMLIKRGAIPLSYLKPSWDNGLAGQLS
                     KFTLMTLITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPTHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(9785..10915)
                     /gene="rffA"
                     /locus_tag="N7C74_000982"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(9785..10915)
                     /gene="rffA"
                     /locus_tag="N7C74_000982"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HGU2430902.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCLQNAHMFYIKLRDIGDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(10920..11594)
                     /gene="rffC"
                     /locus_tag="N7C74_000983"
                     /gene_synonym="wecD"
     CDS             complement(10920..11594)
                     /gene="rffC"
                     /locus_tag="N7C74_000983"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HGU2430903.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPFLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11572..12453)
                     /gene="rfbA"
                     /locus_tag="N7C74_000984"
     CDS             complement(11572..12453)
                     /gene="rfbA"
                     /locus_tag="N7C74_000984"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HGU2430904.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEVATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12472..13539)
                     /gene="rffG"
                     /locus_tag="N7C74_000985"
     CDS             complement(12472..13539)
                     /gene="rffG"
                     /locus_tag="N7C74_000985"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HGU2430905.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPQGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWRKQVQDGSYQGERLGLKG"
     gene            complement(13539..14801)
                     /gene="wecC"
                     /locus_tag="N7C74_000986"
     CDS             complement(13539..14801)
                     /gene="wecC"
                     /locus_tag="N7C74_000986"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HGU2430906.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(14798..15928)
                     /gene="wecB"
                     /locus_tag="N7C74_000987"
     CDS             complement(14798..15928)
                     /gene="wecB"
                     /locus_tag="N7C74_000987"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HGU2430907.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(15984..17030)
                     /gene="wzzE"
                     /locus_tag="N7C74_000988"
     CDS             complement(15984..17030)
                     /gene="wzzE"
                     /locus_tag="N7C74_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HGU2430908.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(17042..18145)
                     /gene="wecA"
                     /locus_tag="N7C74_000989"
     CDS             complement(17042..18145)
                     /gene="wecA"
                     /locus_tag="N7C74_000989"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HGU2430909.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGNLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFLLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18385..19644)
                     /gene="rho"
                     /locus_tag="N7C74_000990"
     CDS             complement(18385..19644)
                     /gene="rho"
                     /locus_tag="N7C74_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HGU2430910.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19729..19830)
                     /gene="rhoL"
                     /locus_tag="N7C74_000991"
     CDS             complement(19729..19830)
                     /gene="rhoL"
                     /locus_tag="N7C74_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HGU2430911.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(19971..20300)
                     /gene="trxA"
                     /locus_tag="N7C74_000992"
     CDS             complement(19971..20300)
                     /gene="trxA"
                     /locus_tag="N7C74_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HGU2430912.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20431..21696
                     /gene="rhlB"
                     /locus_tag="N7C74_000993"
     CDS             20431..21696
                     /gene="rhlB"
                     /locus_tag="N7C74_000993"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HGU2430913.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHCLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            21832..23316
                     /gene="gppA"
                     /locus_tag="N7C74_000994"
     CDS             21832..23316
                     /gene="gppA"
                     /locus_tag="N7C74_000994"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HGU2430914.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(23363..25384)
                     /gene="rep"
                     /locus_tag="N7C74_000995"
     CDS             complement(23363..25384)
                     /gene="rep"
                     /locus_tag="N7C74_000995"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HGU2430915.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            25601..26050
                     /locus_tag="N7C74_000996"
     CDS             25601..26050
                     /locus_tag="N7C74_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HGU2430916.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGPFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNAFIVVPLSTTRDHDKLKRGMHVEIASNVINDLQFFDQQIRWAKTD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            26249..26530
                     /gene="ppiC"
                     /locus_tag="N7C74_000997"
     CDS             26249..26530
                     /gene="ppiC"
                     /locus_tag="N7C74_000997"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HGU2430917.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(26576..28051)
                     /gene="ilvC"
                     /locus_tag="N7C74_000998"
     CDS             complement(26576..28051)
                     /gene="ilvC"
                     /locus_tag="N7C74_000998"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HGU2430918.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVHTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDLNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            28201..29094
                     /gene="ilvY"
                     /locus_tag="N7C74_000999"
     CDS             28201..29094
                     /gene="ilvY"
                     /locus_tag="N7C74_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HGU2430919.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(29146..30690)
                     /gene="ilvA"
                     /locus_tag="N7C74_001000"
     CDS             complement(29146..30690)
                     /gene="ilvA"
                     /locus_tag="N7C74_001000"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HGU2430920.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(30693..32543)
                     /gene="ilvD"
                     /locus_tag="N7C74_001001"
     CDS             complement(30693..32543)
                     /gene="ilvD"
                     /locus_tag="N7C74_001001"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HGU2430921.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHSDRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQISFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(32608..33537)
                     /gene="ilvE"
                     /locus_tag="N7C74_001002"
     CDS             complement(32608..33537)
                     /gene="ilvE"
                     /locus_tag="N7C74_001002"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HGU2430922.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(33557..33820)
                     /gene="ilvM"
                     /locus_tag="N7C74_001003"
     CDS             complement(33557..33820)
                     /gene="ilvM"
                     /locus_tag="N7C74_001003"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HGU2430923.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(33817..35463)
                     /gene="ilvG"
                     /locus_tag="N7C74_001004"
     CDS             complement(33817..35463)
                     /gene="ilvG"
                     /locus_tag="N7C74_001004"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HGU2430924.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLKLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(35466..35516)
                     /gene="ilvX"
                     /locus_tag="N7C74_001005"
     CDS             complement(35466..35516)
                     /gene="ilvX"
                     /locus_tag="N7C74_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HGU2430925.1"
                     /translation="MNNSTKFCFPRFRTGN"
     gene            complement(35603..35701)
                     /gene="ilvL"
                     /locus_tag="N7C74_001006"
     CDS             complement(35603..35701)
                     /gene="ilvL"
                     /locus_tag="N7C74_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HGU2430926.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
BASE COUNT         8647 a         9853 c         9045 g         8183 t
ORIGIN      
        1 taattacgtc atcattttgt tgacatgaaa ccggagcatt ttgcatggaa ttttcgtttt
       61 cacccaaact tcttgttgtt gctgtcgccg ccgctcttcc tctcatggcc agcgcagcag
      121 ataccccgtc aactgccacc gcgcgcaaag gctttgccgg atacgatcac ccaaaccagt
      181 atctggttaa accggcgacc actattgccg acaacatgat gccagtaatg cagcatccgg
      241 cgcaggataa agaaccccag cagaagctgg cagaacttga gaaaaaaacc ggtaagaaac
      301 cgaatgtggt tgttttcttg ctggacgatg tgggctggat ggacgtcggt tttaacggtg
      361 gcggcgtggc ggtgggtaac cctacaccag atatcgacgc cgttgccagc caggggctga
      421 ttttaacttc ggcgtattct caaccaagct cttccccaac ccgcgccacc attctcaccg
      481 gacaatactc catccaccac ggcattctga tgccgccaat gtacgggcaa ccgggcgggc
      541 tgcaagggtt aaccacgctg ccgcagttgc tgcacgatca gggctacgtc actcaggcca
      601 tcgggaaatg gcatatgggg gaaaacaaag agtcgcagcc gcagaacgtt ggctttgatg
      661 atttccgtgg ctttaactcg gtgtctgata tgtacaccga atggcgcgac gttcacgtca
      721 atccggaagt ggccctgagt ccggaccgtt ctgaatacat caagcaatta ccatttagca
      781 aagatgatgt tcatgcggtg cgcggcggcg aacaacaggc cattgccgac attacgccga
      841 aatatatgga agatctggat caacgctgga tggaatatgg cgttaagttc ctcgacaaga
      901 tggcgaagag tgataagcct ttcttcctct attacggcac tcgcggctgt cacttcgata
      961 actacccgaa cgccaaatat gcgggtagct ctccggcacg cacctcgtat ggcgactgca
     1021 tggtggaaat gaacgatgtg ttcgctaatc tgtataaagc actggagaaa aacggtcagc
     1081 ttgataacac gctgattgtc tttacctccg ataacggacc ggaagccgaa gtaccgccgc
     1141 acggacgcac accgttccgt ggtgcgaaag gatctacctg ggaaggcggc gttcgcgtac
     1201 cgactttcgt ttactggaaa ggcatgatcc aaccgcgtaa atctgacggt attgttgatc
     1261 tggcagatct cttcccaaca gcactggatc tggcggggca tcctggtgcg aaagtggcga
     1321 atttagtgcc gaaaaccacc tttatcgatg gtgtggacca gacatctttc ttcctgggaa
     1381 caaatggtca gtctaaccgt aaggccgagc actacttcct caacggtaaa ctcgctgctg
     1441 tgcgtatgga tgaattcaag tatcacgtcc tgattcagca accttacgct tatacccaga
     1501 gcggatatca gggtggattc accggcacag taatgcaaac agccggatcg tcggtgttta
     1561 acctctacac cgatccgcag gagagcgact ctatcggcgt gcgccatatt ccgatgggtg
     1621 taccgctaca gaccgaaatg cacgcgtata tggagatcct gaaaaagtat ccaccacgcg
     1681 cgcagattaa atctgattaa gccggcgcta tcgctgagat ctgcctttgc cggatgcgat
     1741 gctgacgcat cttatccagc ctacagaacg ctgcaattta ttgaatttgc acgatcatgt
     1801 aggccggata aggcgtttac gccgcatccg gcaatcaacc gcaggcgacc acaaaatggc
     1861 cgctttttta cttactcacc accagcaaat gcgcctgcat aatgtcgctg gccgggcgac
     1921 cgtgcgccag caaatcagcc attgctttaa gatatggcgg tagatggcgg aaataacgct
     1981 gatacccggc acacaaataa ttcagtcccg gtttgccgct ggcatcgagc atgaagcggt
     2041 gtttcgggca gcctccccaa catgctttta acacgttaca actgcgacac tgcgccggta
     2101 attgcttaaa tttatcttca ccaaacacct gctgttgcgg ggaatcgatc atttctgcaa
     2161 ttgtttgctg gtgcatattc cccagtcgat attgcggata aacatagtga tcgcaggcgt
     2221 aaacgtcgcc attgtgctca acaatcaccg agcgcccaca ggttggctga tgatggcaaa
     2281 ccgcacccgg cgcaccgaca aaattggcaa acgcccattc gatattcatc acgaaaatct
     2341 tgccgacgtc gcgtttaatc cagtggtcga atatcgccac cagaaattca ccaaattcat
     2401 cggggcgcac agaccattcc gtcagttccc cctgaatatc accaggcgca tgtagtttca
     2461 gtccttcgcg ggcagtcgtt tcatcagcca ggcgctcgac caccggaata aactggatga
     2521 attcgactcc cgcatcgcac aaaaaatcat aaacctgtaa cggttgctgc gcgctggtgc
     2581 ggttgacgca gaccagcacg ttatagtcga catgatgttt ttgcaggagc gtcagggcac
     2641 gcatcaccag tttatgcgtg ggtctgccgc cttttgtcac gcgatattga ttgtggatct
     2701 cagccggacc atccagcgat aacccaacaa gaaaatgatt ttctgccaga aatgcacacc
     2761 attcatcatc gagcagtacg ccgttagtct ggaagctgtt acttatcttc ctgccagcac
     2821 catatttcgc ctgtagcgcc acggcacggc ggtaaaactc cagcccgagt agcgttggtt
     2881 cgccgccctg ccaggtaaaa gccacttcgt tttgcggttc gctggcagcg atatagtgac
     2941 ggacatacgc ttccagcgtg tcatcgtcca tatgcgtgac tggcttttcg cggtaaaggg
     3001 attgtttttc gagataaaaa cagtagtcac agttcagatt acaatcggaa ccgctcggtt
     3061 tcgccatcac atgaaaagca cgcgttggaa cctgttgcag catggtcgct ccttgcctga
     3121 agtcatcctt caatacgtta agggcgaaat cataccgtgg ggcaatgagt tattacgggc
     3181 gtttattgcg aagggagggt gacgaaatgc acagaaaaca aaaaaccccg ccgaagcggg
     3241 gttcaaaatt ggtcggcgag agaggattcg aacctccgac ccactggtcc caaaccagtt
     3301 gcgctaccaa gctgcgctac tcgccgaaat actgcttttt gaatttttag ttcaattctt
     3361 taaagtcgtg gtgcgagggg ggggacttga acccccacgt ccgtaaggac actaacacct
     3421 gaagctagcg cgtctaccaa ttccgccacc ttcgcattgt cacaacttct aataatgggg
     3481 tggctaatgg gattcgaacc cacgacaact ggaatcacaa tccagggctc taccaactga
     3541 gctatagcca ccactacaaa tcttgttacg cggtattact accaccgcag ctcaagcgcc
     3601 gggactaaat ggcgcgcccg acaggattcg aacctgagac ctctgcctcc ggagggcagc
     3661 gctctatcca gctgagctac gggcgcttag cgccgttgcg ggggtggata atacggactt
     3721 cccccccctc tgtctagtcc ctttttaaat aaattgcgcg tttggttacg gtttgtgcgt
     3781 tttgctgctt attcctccag tttatgcgct ttcccgtggc gattaaggcc aaaaacttta
     3841 taaatcgccg tcaccgccag cataaagatg atgccaacaa acagcgacat acgcgtatct
     3901 tcattaaagt acatgccgat caaaacgcaa atcaggaatg ccattgttac gtagttggcc
     3961 cacgggaaca ggattgagcg gaacggatgg ctggcaatcg ccgctttatg tgcacgccga
     4021 aaacgcagct ggcttatcaa tatcacaaac catggcacca tccccggaag cacgctggca
     4081 ctgtagacgt agacaaacac acgctgcgga ttgggaatga tgtagttcag gcatgagcca
     4141 attagcagaa tagcaataga tactgccaca cccgcaaccg gtacgccgtg acgggaaact
     4201 ttcgccatcg ccgccggtaa ctgacggttt ttcgccagtg cgtagagcat acgtccgcaa
     4261 ctgtacatgc cgctgttaca gccagagagc gcagccgtca gcaccacaaa gttgataatg
     4321 cccgccgctg cggtaatacc gattttggca aaagtcagta cgaacgggct gccgttgctg
     4381 cctatttcat tccacgggaa gatggtgacg ataacgaaaa tcgcgccaac gtagaaaatc
     4441 aggatccgcc acagcacctt gcctacggca ctgcgcagcg tcacctgcgg attcttcgct
     4501 tcaccggcag taatgccaat cagctccacg ccctggtagg acgccaccac aatacacagc
     4561 gcggtcagga accctttcca gccacccgca aagaaaccgc catgctctgt gagattgcta
     4621 aaaccaatcg actgcccgcc attgccaaag ccaaagaaaa tcacgcccag gccaatgaca
     4681 atcatcacga taatcgtggt gactttgatc atcgcgaacc agaactcgat ttcgccgtac
     4741 aaccgcaccg ctgccagatt cgccaacgcc accagcgcca ctgcgatcaa tgcgggtatc
     4801 cactgcgcca tctccgggaa ccagaactgg acataaacgc caatggcggt gatttcagag
     4861 atccccaccg ccatccacat aaaccagtaa gaccaggcgg tgagatagcc aaagaacggg
     4921 ctcatataac gatgcgcata aacggcgaac gaaccggtaa ccggttcgag gaacaacatt
     4981 tcgcccattg aacgcatgat gaaaaagacg aacagcccgg cgatgatata ggccaacaat
     5041 acggatggcc cggcccattt cagggtactg gcggccccca taaacaggcc gacgccaatg
     5101 gtgcccccca gggcgatgag ttcgatatgt cgagcttcca gcccacgctg tagctctggt
     5161 ttgttatctg ccataaatcc tcgtgttgtg tttgcatgct ttccggtgtt accggttatc
     5221 gttatgggta catcgagtgt tgcgaatgtt ttcgtaattc aggagaaatg gcaaataaag
     5281 cattaaaaat ttgaatgctt tgtgtaataa aaaagcagac aggcgacgga gtgaccactc
     5341 cgtcgcttta caaagagagg aaaatcagag gttgccggtg taatgccagc gtaaataacg
     5401 cagcaaacga agctgacgct taatgcggct cggctgcgaa agcaggcggt agagccactc
     5461 cagccccagc gtttgccaga ttttcggtgc gcgttttacg tgaccggtga aaacatcgta
     5521 agtcccgcca acgcccatat acagcgcatc tggatgcacc agacggcagt cgcgcatgaa
     5581 gatctcctgc tttggcgatc ccatcgcaac ggtgacgatt tgcgctccgc tggcatgaat
     5641 gcgttcaaac agcgcctgac gctgctcggg tttaaaataa ccatcctgac tgccaacgat
     5701 attcacattc cactggttgc gcagtttagc ttcagtttgc gccagcactt caggtttacc
     5761 gcccacaaga aataccgtcg tcccttcttt gcctgcgcgc gccatcagct cttcccagag
     5821 atcggcaccg gcaacgcggg aaacctgcgc ctgcgggtac tttttacgta ctgaacgtac
     5881 aacgctgatg ccatccgcat atttaaattc ggcagcgtta attaactccc tgacctcggc
     5941 gttctcttca atggtcagca ttttttcagc attaatggca accagcgttc cctgcttaag
     6001 ctgcccgtca gcaaacagat aatcgagggc atgctgcata tcacgccaac caatcaactg
     6061 taagccacgc agcgtatagg tcggtgccgt ggtgttgtta ttcattgtta tccttcaacc
     6121 tgcgtccgga gcgatgattt tgtacgttta tgaatgagtc cggcgctttc aaaaagccag
     6181 tacaacagtt ttgcgatcat cagacatgcg ccgaagacca cgataaaaaa gaccacgcgt
     6241 gagacaaacg aatccagccc ttcacgcgcc agcacgatca tattgaagat cgccccaaag
     6301 cagaaactgt gcaatatcgc agccttatag cgattagttt cgcggttgcc cagctcatac
     6361 agccagtcga accatttgat gatcagtcca accacaatcg ccccgagcgg gatgaacaac
     6421 gcgccgccca tcaccactag tgagcctata agcgtaggcg agatcgccag tccggagtgg
     6481 ttattcagca cttcccaggt aaagtagttg gctgagttca gcaccatact cgggcgaccc
     6541 ggccacagcc aggaagggat aaagacatag aaatcgcgga caattggcgc caggccctgg
     6601 aagtcgatgt tgtcgtagtt ctgcaacagc aacgccagat tctcccacgg cgagaaggtg
     6661 tcgcgagtga gatagagaaa cgtatagaac gcttcatcgc cgctcacatt cattccatag
     6721 cgttttagcg ccagccagaa catgccaaca atccccagca cgcccgccgc cgccagcatc
     6781 cacaacgaaa tccagccgcg aataatgcca ataaacagga agatagcgaa tgcgatgatg
     6841 atattggcgc gagtgccgcc gacaatcata taagtcagca agccaaaggc gaccgtgctg
     6901 acgaggaaaa acagccacgc tttgctgtcc tggcgcagaa agtagaccac cagcatcgcc
     6961 gggatgaaaa agtaaaagaa gcgttttaac gccacgccgg agacttcact ggaaaagatc
     7021 tgactgtagg agttaagccg gaacagcaaa aagccgttgt gcatgaagaa gatgccgacg
     7081 cttaccagcg cgatacccat caggatcacc cacgtaagat tggtttccac gcggttcatg
     7141 gtaaacagcg gacggcgcgg tacatcagca acgcgtttgc gtaggcgggt tttgtaggtg
     7201 acgtagtaaa ccgcgtagaa gcagcccgca gaaagcaacg cctgcaacag gatttctggc
     7261 ggcgcgacac caacatcaaa gcgaaacacc agcacgctgg tcagcgggaa gccaaagaaa
     7321 aaggtgagca gaaacagcaa tgaaaagaat acattgaagt taaagcgcac acgacgaaac
     7381 tcaaaccagg tcagcgtggc aataaacagc gtgcagagca gccagacaac aaacaggcca
     7441 ctgaattgca gcagactcat gcgacctccc cggcggcaat cgccaacgcc cgctgccagc
     7501 cttgtagata gttagggcta aagaaggcaa tggtgttttt atccaccgac gccaactggc
     7561 gctgcgcttc acgcacaata tcctcgttga gatcgtcggt agtaaacagc accggtaaat
     7621 gctgttccgt catatcctgc cagaacggat tttcccggtt aagcacacaa ggaatgcccg
     7681 cctgaatcag taagcacagc gtaccaatgc cctgctggcg ggcaaaaata aagtaaccaa
     7741 gatcgcactg acgaagtagc gtcagatagg cgtcaaattc cagtttttca ctcagaactt
     7801 gcagattttc ttcgctgaat aactccagcc ccgcctgacg aacttcctcg atgtacgctt
     7861 cgttatgagg cggatatccc atcggcacca ccacttttac cgtatcgcca aattgctgat
     7921 gaacggcgcg caaggcagca atatgctcat tgctgcggtc gccggagttc cccaccagaa
     7981 tggtcatttt cccttcacgt tgccgatcgt tcgccatcgt attgagagaa gcatccatcc
     8041 gcgtcgggaa gtacagcagt tcgccccgca cctttgggtg cgttttggca aaaaagctca
     8101 aatcaccacg ggtggcaaat acacagccga ctcgcttttg cgccaggcga cgtagtgggt
     8161 aaaaaagctt atatctcaag ccactggaaa gctcgtacag gtctgccccc cagatatgcc
     8221 agtaaaactg gctgggctta atgccaccac tcagcagagc cagccacagt gtgggattga
     8281 actgaccgtg gaagaaaaaa cgctgctgac ggttagcttt tgctttcgcg atgaccgctt
     8341 ccgccagcga ttttttccca gggaaaaatt gcacagaaag cgccggacag ctatcactta
     8401 agccgtcgtc cttgccaaca accataaact cgcgcgcgtg ctcgctcgtc gcggccagcg
     8461 cgtcattgaa aaaccgcaaa acggttcggt tatggtgagg gatatccgat cccagtacgt
     8521 gaatcagtac agtcatgccc gcctacgcca gagtaaaaac acgccacaac aaagagaaaa
     8581 atagacgata taggttgcca tatatgcctg tgccgcgccc agcgcgccgt gcgtagggat
     8641 tagccagtgg gcaaatacca tcaataaagt gaactggctg acttccgcca gaatataaaa
     8701 ccgcagtgaa gctttggcga tcaccagata accaaagaca taagcgccca cttttaacac
     8761 atcacccacc aactgccagg caaagagatc gcgcatagcg gtaaatttat tcgacaacag
     8821 cagccagata gcaaaatcac gcagcaacca gacggtaaaa ctcgccgccg ccactgccgg
     8881 taagacgaat ttcagcgatt taaccacttc ccgggtgata tcgcgctttt ccgttagccg
     8941 cgacaacgtg ggcagcaaat atacgctgaa cgatgccgta ataaattgca ggtaggcatc
     9001 ggaaatactg ctcacccctt gccagatccc cacctcatcc cagctatact gcgccgccag
     9061 cagtttacgc atcatgatgt aagcaacagg caaggtcacc gacgtaatca acgtcatgag
     9121 cgtaaatttg ctcaactgcc ctgccagacc gttatcccag ctgggtttca gatagcttaa
     9181 cgggatagca ccacgcttaa ttaacatgac ggcggcagga atcaccacca gcgcgggaat
     9241 caacgccaga cccagcaacg ccccttcata tccgcccaaa cggtaactga cgtagtacgc
     9301 aagaacgcca atcaagctgc caacaatcag agataacgca ttacctgcgg catcgcgaaa
     9361 gcctttcatc aacgccagta acaggttgcc ccaggcgatc cccatttgca ccagcgccac
     9421 taaacgcacc agcccctgat agtcgtgatt accaaacaat cccaagctga ttggcgcagc
     9481 tgccagcaca aaaaccagcg ccatcagcgt agagaagcca agtaccatcg ctgatgaagt
     9541 gccgaccacg cggcgcagct gttgcggatt atcatggtac tgggcaacgt atttggttac
     9601 accgttaaag atgccagccc cggcaagcac gccgagcacg gtaatcaact ggcggaaatt
     9661 tgccgccagc ccaagccccg ccggaccaaa tgacaccgcc agcaacttac cgaccagtaa
     9721 cccggcacca atcttgacca gtgtactggc cgccgtccac aaggacgctt ttgccaacga
     9781 catatcagga gaagtagttc aacaaagtcg caattaccgt acgctgattg acgggcgaca
     9841 ggttgtagaa cagcggcagg cgcagcaggc gctcgctctc tttggtggtg tagcgatctt
     9901 caccgtggaa ctcaccaaag cgttcccccg cagggcaacc gtgcagcgga atgtaatgga
     9961 acaccgccat gatttccgct tctttcagaa agttaatcaa cgcgctccgg tcaccaatat
    10021 cccgcagttt aatgtaaaac atatgcgcgt tctgcaagca gccatcggga atcgacggca
    10081 gctcgatacg cccggctttc gccagaggtg ctaacgcatc gtagtagttt tgccacagcg
    10141 ccagacgttg ctggttgata cgatccgctg cttccagttg cgcccacagg tacgcagctt
    10201 gcagatcgga catcaaatag ctggagccga tatcgcgcca ggtatattta tcgacctgac
    10261 cacggaagaa ctggctgcgg tttgtgcctt tttcacggat gatctcggct cgttcgatca
    10321 acgctttatc gttaatcagc gtcgcgccgc cttcaccgcc cgccgtgtag tttttggttt
    10381 catggaagct aaagcagcca atatgaccaa tggttcccag tgcacgccct ttgtaagtgg
    10441 acatcacgcc ctgagcagca tcttccacca caaacagatt atgctttttc gccaacgcca
    10501 taatggtgtc catttcgcag gccacgcccg cgtaatgcac cggcacgata acgcgcgttt
    10561 tgtcggtgat cgccgcttca atcaacgttt cgtcgatgtt catggtgtcc gggcgaacat
    10621 ccacaaaaac gatttttgcg ccacgcagca caaaggcatt ggcggtggag acaaaggtgt
    10681 agctcggcat gatcacttca tcgccaggct ggatatcgag cagcagcgcc gccatctcca
    10741 gcgaagcggt gcaggacggc gtcagtaaca ctttggcgct gccaaaacgt tgctccagcc
    10801 actgctggca gcgacgggta aaaccgccat cgccacacag tttgccgcta cccattgccg
    10861 actgcatata gtcgagttcg gttcccacca ccggcggtgc gttaaatgga atcatgtgat
    10921 cacctgtata accagtacgc ggtgctttct acattcgcac cactttgtat gtatcgttta
    10981 agcgcggcgg tgttgcccat ttgggtcgcc acccgcaaag ttgttttacc gcgagcatac
    11041 gcccagttta gcgccgtttg catcagctca gcacctgcac cgcgtccagc cagcaggcca
    11101 attcgcgcat ctgtagcatt gagttcccgt aaagagacat aaccgcgaat atcgccggac
    11161 gccgcacgta aaatcagaca ttgatgatca aaggtgccgc gcacggcatt ttcaatccac
    11221 tgtgcataaa agcgactgct ggcgtcaggc gcataccacg gcgcacgaaa acggctttgc
    11281 gcaaatgcgg cgctggctaa ctgacgtaat gcgggaatat cggtctcttg tgccactaca
    11341 gcaccgctat cactggcatt gttcacgggt agcgccaaat caacttcacc ttctaccagg
    11401 gagaatccca gctgttgcag ggcatccagt tcccccgtat ttgatgccgc aattttggcc
    11461 tgcacccgtg accacggcgc taacgcgtct ggcgtcagga acggtgcttc agacgtaatg
    11521 cgcacgatgg cgctgttaac accaaagaag gcgttttccc aggttagtgg ctcaatactg
    11581 gcgcggacgg gcacgaagta actccagcag atattggccg tagccagttt tcgctaatga
    11641 actggcagca cgcttcacac cctcgtcatc gagccagccg ttacgccagg cgatctcttc
    11701 caggcaggca atcttaaagc cctggcgttt ttccaccgtc tgtacaaagg tgctggcttc
    11761 aatcaggctg tcgtgagtgc cagtgtccag ccaggcaaat ccgcgcccga gcagttcaac
    11821 ggtcaggttg cccgcctcga ggtacatctg gttgatggag gtaatctcca gttcaccacg
    11881 ctccgacggc ttcacctgct ttgcgtactc cacgacttta ctgtcgtaga aataaagccc
    11941 ggtcaccgcc cagtttgatt tcggctgttt tggcttttct tccagcgaga tagcgcggaa
    12001 attatcgtca aactccacca cgccaaagcg ttccgggtcc atcacctgat agccaaaaac
    12061 cgtcgccact tccgtgcgcg ccgcaacatg acgcagcttc ggactgaagc cctgaccgaa
    12121 gaagatgtta tcgcccagca ccagacaaga aggttcacca ttaaggaagg tttcaccgat
    12181 gataaaggcc tgcgccagac cgtccgggct gggctgttcg gcatattcca gctggatacc
    12241 gaactcacta ccatcgccca gcaggcgctg gaaataacct ttatcttccg gcgtagtgat
    12301 gatgagaatt tcgcggatac cggccagcat cagcaccgac agcggatagt aaatcattgg
    12361 cttatcgtaa atcggcaaca gttgcttcga tacgccgcgc gtaatcggat gcaatcgggt
    12421 accggaaccg cccgccagga taataccttt catgtttgcc tccgctgaaa attagccttt
    12481 cagacctaaa cgctcgccct gatagctgcc gtcctgcacc tgcttcctcc agctttcatt
    12541 agccagatac cactgcaccg ttttacgcat tccactttca aaggtttcct gcggcagcca
    12601 gccaagttca cgggcaattt tcgaagcatc aatggcatag cgcagatcat gccccggacg
    12661 gtcagcgaca aaggtgatca agtcacgata atgcaccacg ccttgcggct tgttcggagc
    12721 cagttcttcc agcagctcgc aaatggtttc cacaacatcg agattcttac gctcgttgtg
    12781 accaccaata ttataggttt caccgacttt cccggtggtc gccacgcaat acagcgcgcg
    12841 ggcgtgatct tcaacataca gccagtcacg gatttgctgc ccgttgccat ataccggtag
    12901 cggtttaccc gccagcgcgt tgaggatcat cagcgggatc agtttttccg gaaagtggta
    12961 aggaccgtag ttattcgagc agttggtgat aagcgttggc agaccatagg ttcgcagcca
    13021 ggcgcgcacc agatggtcgc tgctggcttt tgacgcggaa taagggctgc tcggcgcata
    13081 cggcgtggtt tcggtgaaga aatcatccgt cgagtgcagg tcaccatata cttcgtcagt
    13141 ggagatatga tgaaaacgga acgctgattt tttatcttcc gtcagcgcat tccagtaagc
    13201 ccgcgccgct tcaagcaatg tataagtccc gacaatgttg gtttcaataa acgctgccgg
    13261 gccgtcaata gaacggtcaa catggctttc ggctgccaga tgcatgacac agtctggctg
    13321 atgctcagtg aatacgcgcg ccagttctgc ccgatcgcag atatcaactt tctcaaaggc
    13381 aaagcgctcg ctttgcgcga ccggtgccag cgacatcagg tttccggcgt aggtcagctt
    13441 atcgaccact accaccgcgt cgctcgtttc gttgatgata taacgcacca gcgccgagcc
    13501 aataaacccg gcaccgcctg ttatcagaat ctttctcatc agcgccagac tcctttagca
    13561 tcgacgacat actgctgatg gacattgtcg ccattgataa ctttgaactg actatgatcg
    13621 accagcatca ccagcacgtc tgccgttgcc agcgcctcgt caagctgcgc cagagtacaa
    13681 agcccggtca gttttttcgg caactggtgg atgttaggct caacaaccag agtttcgccg
    13741 ctatgccact gggcgatcag ttcagcgatt tccatcgccg ggctttcgcg caggtcatca
    13801 atattcggtt taaacgccag accaaagcag gcgattttca gttcactggc gcgtttatcg
    13861 gtagccgcca ggcaatcagc caccgccgct ttcacctgat cgataaccca gaacggtttg
    13921 tgatcgttca cttcgcgcgc ggtacggata agccgcgcct gctgggggtt ctgtgccacg
    13981 ataaaccacg gatcaacagc aatgcagtga ccgcccacgc cagggccagg ctgaagaata
    14041 ttaacgcgag ggtgacgatt cgccaggcga atcagttccc agacgttaat cccctgatcg
    14101 gcacaaatca gcgacaattc attagcaaaa gcgatattca catcgcggaa gctgttttcg
    14161 gtgagcttac acatttccgc cgtccgcgag ttagtgacga cacactcacc ttcgaggaaa
    14221 attttgtaca gttcgctggc gcgggccgaa caaaccggcg tcataccacc aatcacgcga
    14281 tcgtttttaa tcagctcgac cataacctgg cccggtaaca cgcgttccgg gcagtaagca
    14341 atgttgacgt ccgcctgctc gcccacctgc tgtgggaaag tgagatccgg acgcatctct
    14401 gctaaccatt ctgccatctt ctcggttgac cccaccggcg aggtggattc aaggatcacc
    14461 agcgcgcctt ttttcagcac tggcgcaatg gagcgagcag ccgattcaac gtaggtcata
    14521 tctggctcat gatcgccttt aaacggcgtg ggtacagcaa tcagccaggc atccgcttca
    14581 actggcgtcg tgctcgctcg taaaaaaccg ccttctacgg cagtttttac tacgctcgcc
    14641 aaatcaggct cgacgatatg gatttcgcca cgattgatgg tatcaaccgc gtgttggttg
    14701 atatcgacac caatcacctg tttttgccgt gaggcaaacg ctgctgctgt aggcagcccg
    14761 atgtaaccca gtccgataac agaaatggtc gcaaaactca tagtgatatc cgattatttt
    14821 ttaacgcttc cagaatgcga gagcatgcct gaccatcacc atacgggtta tgggcgcggc
    14881 tcatagtttg atattcgttt tcgtctttta aaagacgcgt cacttcctcg acaattcgct
    14941 gcttatccgt gcctaccaga cgcaccgtac ccgccgtcac cgcttccgga cgctcagtgg
    15001 tatcgcgcat caccagcacc ggtttcccca gcgaaggcgc ttcttcctga atgccgcctg
    15061 agtcggtcaa aatcagccag gcgtggttca tcagccagac aaacggtaaa tactcctgag
    15121 gatcgatcag aatgacattt ttcacatgcc ccagaatgcg attgaccggc tctctgacgt
    15181 tcgggttgag atgcaccgga tagacaatct ggatgtcctg gtgcgtggtg gcgatgtctg
    15241 ccagcgcgtg gcagatttct tcaaagccac gaccgaagct ttcacgcctg tgaccggtca
    15301 ccagaatcat ctttttatcg gggtcgataa acgggtaatt tgccgcaagt tctgaacgca
    15361 gcgtgtcgct gctcatcacc tggtcacgca cccataacag tgcatcaatg actgtattac
    15421 cggtaatgaa gattcggcta tctgcaacgt tttcacgcag caagttttgc cgggaagttt
    15481 cggttggaga gaaatgatac atcgccagat gcccggtcaa tgtacggtta gcctcttccg
    15541 gccacggcga atagagatcg cccgtgcgta aaccagcctc aacgtgacca acaggaatac
    15601 gctgataaaa cgccgccagg ctggttgcca gcgtcgtcgt tgtgtcgccg tgaaccagca
    15661 cgacgtctgg tttgaactcg gcaagaatag gttttagccc ttccagaatc cgacaggtta
    15721 tctctgtcag gccctgtcct ggctgcatta tgttgagatc gtagtcaggt acaatggaaa
    15781 agagtttcag cacctgatcg agcatctccc gatgctgcgc agtgacgcaa actttagcct
    15841 caaaaaaagg atcttttgcc aacgcatgca ccaacggagc catcttgatg gcttccgggc
    15901 gcgtaccaaa tacagtcagt actttcacat cgattctctt cgaataagcg gcgagcgcct
    15961 ttgcgctcac cgcagcagtg ttgctatttc gagcaacggc gggttaatgc gacaccagcc
    16021 ccgatcagcc ccccgacaat gccccacata atcatcagga aggcacgacg tgggctatcg
    16081 cgttttaccg gttcttccgg cgtacgcaaa tagcgatagg tctgaaaacg cggatccagg
    16141 gttggaccaa cattcagggt gtttaacatg gcccgattct gatcatagtc gagatcaaag
    16201 gccggaccga cggcctgtaa attttccagt cgagcctgga gcattggacg cccaagcagg
    16261 aacatttctg aatcaggtaa ttcctcggca ggcacatctg tcgcactgcg cgaaatatta
    16321 tgctgctcag caattttcag cgcctgctca atgctgttca tccggcggtc gtagatggct
    16381 ttcgccacct cttcctgacg cttcacctgt gctttcatct ggatggtacg cgccgcccat
    16441 gcgcctttca gctcatcatt cagatggctg gctgcacgct ggctggcaaa agcaacatac
    16501 tgacgtaaca ggttattagc gtcaggcgcg gtttcggcaa taagcttcac gctgtcattg
    16561 accgcgcggg taaagtctcc ggggataaac tggatgttgt taatcatttc atccagcaac
    16621 gccgcatcgg ctttgctgtt gcccaccatc cgctgcttgt aatagtcggt ttgcagccag
    16681 aactctctgc gggtatccca cgaggccagt tgcataacaa actctttata ggcttcgtcc
    16741 atgaccgatg gttggtcggc agaagccatg tttgaacgga catccaggtt acgcaaaaat
    16801 tgctgctgcg agtaatatcc ccccagcata ttcaccgttg gacgatcggt aatcgccgtc
    16861 gagctccact cctgacgagc aaaaaacgta tacgccagcg cgattaacgc aaacgccagc
    16921 cccatgccaa taatccatag cttcccagcc cacaaggtac gaaacaaccc acgaatatcc
    16981 agttcatttt cagcgtcttc ggccggtttc ccaggcattg gttgtgtcat cacatcctca
    17041 tttatttggt taaattgggg ctgccaccac gatttctacg cagtctgcgt tttacgcgct
    17101 taataaagcg agcaactttc caggcacgct taatgcaata tccatagagg aggaatgcta
    17161 gcaaaaagag caccaacatg acccactccg ggacaaaatg agaatattct gccagcacgc
    17221 caatggaagc gagcagtgct gcggcaaggg taatcagcac aaacgcctgg cgggaagtaa
    17281 acccggcacg catgatcaaa tggtgaatat gctgacggtc aggagagaat gggctcatgc
    17341 ctttacgcag gcgacggtac ataatcgcca ccatatccat tagcggaatg gcgattatcc
    17401 acaaagcggt aaccgggctg atgggatggg ttttgccttg ggtcgtttcg agcaggatcc
    17461 agatcacggt aaaaccaatc agcgtactgc ccgcatcacc cataaagact ttgtagcggc
    17521 gacccaggat accaaggtta agcatgatat atggcaggat ggcggcgatc atcgcaaagc
    17581 accagattgc gaggctggtt tgcccgtcga accacaaaat cataccgatt gctgcaaacg
    17641 agacgcagga caacccgccc agcaagccat caatgccatc aaccatgttg aacgcattaa
    17701 tggccgccca gacggcaaat agcgtcagga agtaaccaaa cggtccgagc accatctccc
    17761 aggagccaaa gatataaccc aggctactga gataaaggtt gccaaacacc atcataacaa
    17821 tgccaacagc ggcctgtatg gtggcacgga tttttacgct gatatcaaaa cggtcatcca
    17881 gcgcgccaat gaaaacaagc acaccggcac aagcgagata gagagatgca tgcggaatat
    17941 agtaatcgac aattccgaac gtgaagcaaa tccctgcgta aaccgaaata cccccaacga
    18001 gtggtatcaa cccctggtga cgtttgcgga agtttggttt atccactaaa ccgacttttt
    18061 ttgccacctt acgggcaaaa aacagaaaca gtgtcgtgaa taaaaaaata ctgatgagat
    18121 cagtactcac tgtcagtaaa ttcacaatgc atgctctcag agaaaattat tagcagaagt
    18181 ataaccacga agacctttat tcagaaggga aagtccgttc cgaacccacc agtcctgtgc
    18241 aaaaattaac catttgatat gcaaattgca taacatgcca gcaaattcca ggaattttcc
    18301 taacatcgcc agcgcggcat taagattaca gatataaaag caaaacgcca cgtaaacacg
    18361 tggcgttttt ggcataagac aaatttatga gcgtttcatc atttcgaaga aatcgtcatt
    18421 ggtcttggtc attgccagtt tattaatgag gaattccatt gcatcgattt cgcccatcgg
    18481 gtgaatgatt ttgcgcagga tccacatttt ctgcagttct tcctgagtcg tgagcagctc
    18541 ttctttacgg gtaccggaac ggttgtagtc gatagccggg aagacgcgtt tttcagcgat
    18601 cttacgagag aggtgcagtt ccatgttgcc tgtaccttta aactcttcgt aaataacttc
    18661 gtccatttta gaaccggtat cgataagcgc cgtcgcgata atggtcaggc tgccgccctc
    18721 ttccacgtta cgcgccgcac cgaagaagcg tttcggacga tgcagggcgt tggcatccac
    18781 accaccggtc aacactttac ctgacgccgg aacaacggtg ttgtaagcgc gcgccagacg
    18841 agtgatggag tcgagcagaa tgataacgtc tttcttgtgc tcaaccaggc gtttcgcctt
    18901 ctcgatcacc atttccgcaa cctgaacgtg gcgagatgcg ggttcgtcaa aggtagaagc
    18961 aacaacttca ccttttacca gtcgctgcat ctcggttact tcttccggac gttcgtcgat
    19021 cagcagaacc atcagcacac aatccgggtg gttgtaagca atgctctgag caatgttctg
    19081 cagcagcatg gttttaccgg ctttcggcgg tgccacaatc agaccacgct gaccacgacc
    19141 gataggtgat gccagatcca gtacgcgagc ggttaaatct tcagtagaac cgttaccacg
    19201 ttccatacgc agacgagagt ttgcgtgcag cggggttaag ttctcaaaga ggattttgtt
    19261 gcgggcgttt tcaggtttgt cgaagttaac ttcgttaact ttcagcagcg caaaatagcg
    19321 ttcaccttct ttcggcgggc gaatcttacc agagatggta tcaccagtgc ggaggttgaa
    19381 acggcggatt tggctagggg aaacgtagat gtcatcagga ccggcgaggt aggagctgtc
    19441 tgcggaacgg aggaaaccaa atccatcctg caatatctcc agtacgccat caccaaagat
    19501 atcttcgcca ctctttgcgt gctgcttcag gatagcaaaa ataatgtcct gcttacgcat
    19561 acgagccagg ttttccagcc ccatattttc gccgagagtg atcagctcag aaaccggcgt
    19621 attctttaat tcggtaagat tcatagtggt gtgagttctt aaacttgggg taaatctcga
    19681 acttaatgtt gtgaatggta tggcaggatc atccatgcct gtttacggtc atcgactcat
    19741 gtcttttcgc tgcctggtca caggagagta cgcagaactg aaacgacaag acggattgag
    19801 tgacgagcca gaaatctgtt cacttcgcat ttaagataaa acgggaagcg ttggaaatta
    19861 caagattcaa acttaataag gtatgtttaa tacgaagtca acactaagtt agcatgactc
    19921 acgccgggcg tccagttttt agcgacgggg cacccgaaca tgaaattccc ttacgccagg
    19981 ttagcgtcga ggaactcttt caactgacct ttagacagtg cgcccacttt ggttgccgcc
    20041 acttcaccgt ttttgaacag cagcagagtc gggataccac ggatgccata tttcggcgca
    20101 gtgccagggt tttgatcgat gttcagtttt gcaacggtca gtttgccctg atattcgtca
    20161 gcgatttcat ccagaatcgg ggcgatcatt ttgcacggac cgcaccactc tgcccagaaa
    20221 tcgacgagga tcgccccgtc cgctttgagt acatccgtgt caaaactgtc gtcagtcagg
    20281 tgaataattt tatcgctcat atataactcc acaggaataa gcctggcgtg ttggtttcgt
    20341 tgttggtgta acattaacca actaaaggtt gactttattt caccggatac gctttcgtaa
    20401 agcaatagta agctgatatt ctaccacact atgagcaaaa cacatttaac agaacagaag
    20461 ttttccgact tcgccctgca tccgaaggtt gtagaagccc ttgaaaaaaa agggtttcat
    20521 aactgtacgc ccattcaggc actggccctt ccgctgacgc tggcgggtcg tgacgtagcc
    20581 gggcaggcgc aaaccggtac cgggaaaacg atggcgtttc ttacgtcaac gtttcattgt
    20641 cttctctctc atcccgcgat tgccgatcgc aaggtgaatc agccgcgtgc cttaattatg
    20701 gcaccgacgc gtgaacttgc cgtgcagatc catgccgatg cggaaccgct ggcggaagct
    20761 accggcctga agctgggtct ggcttacggt ggtgatggtt acgacaaaca gctgaaagtg
    20821 ctggaaagcg gcgttgacat tctgattggc accacggggc gtttaattga ctacgccaag
    20881 cagaaccaca ttaacctcgg tgccattcag gtagtggtac tggacgaagc cgatcgcatg
    20941 tacgatctgg gctttattaa agatatccgc tggctgttcc gccgtatgcc gcctgcaaac
    21001 cagcgcctca acatgctgtt ctccgccacg ctttcgtacc gggtacgtga actggcgttc
    21061 gagcagatga acaatgccga atatattgaa gtggaaccgg aacagaaaac gggccaccgt
    21121 attaaagaag agcttttcta cccttctaac gaagaaaaaa tgcgtttgct gcaaacgctg
    21181 atcgaagaag agtggccaga cagagcgatt attttcgcca ataccaaaca ccgttgtgaa
    21241 gagatctggg gccacctggc ggcagatggt catcgtgtcg gtttgttgac aggcgatgtc
    21301 gcgcagaaaa aacgtctgcg tattcttgat gaatttaccc gtggcgatct ggatattctg
    21361 gttgccaccg acgttgccgc gcgtggtttg catattccgg cagtgacgca cgtctttaac
    21421 tacgatttac ccgatgactg tgaagattac gttcaccgta ttggtcgtac aggtcgcgca
    21481 ggcgcaagcg gtcactctat tagcctggcg tgtgaagagt atgcattgaa tttgcctgct
    21541 attgagacct atattggtca ctcaattccg gtaagcaaat acaatccgga cgcattgatg
    21601 accgatctgc caaaaccgct gcgcctcacg cgcccgcgca caggcaatgg tccgcgtcgt
    21661 actggcgctc cgcgtaatcg tcgtcgttca ggttaaataa aaaatgtcat actcaaaatc
    21721 attcaaactg tatcaagatg gcaagagaat gaatcccgat gagcttactg tagtaagtga
    21781 ttcaggtgaa agaatgcagc caacacagag acagattgaa ggatgaagag tatgggttcc
    21841 acctcatcgc tgtatgcagc cattgatctc ggttcgaata gttttcatat gctggttgtg
    21901 cgcgaggtgg ctggaagcat ccagacgctg acgcgaataa aacgcaaagt gcgtctggct
    21961 gctggcctga atagcgaaaa tgccctctcc aatgaagcaa tggagcgcgg ttggcaatgt
    22021 ttgcgcctgt ttgctgaacg tctgcaagat attcctccct cgcaaattcg cgttgtcgct
    22081 acggcgacgt tacgccttgc cgtcaatgcg ggtgatttta ttgccaaagc gcaggaaatc
    22141 ctcggttgtc cggtacaggt gatcagcggt gaagaggaag cacgtctgat ttatcagggt
    22201 gttgctcaca ccactggcgg tgccgatcag cgcctggtag tggatatcgg cggtgccagt
    22261 actgaactgg taaccggcac gggtgcacaa accacctcgt tgttcagcct gtcgatgggc
    22321 tgcgtcacct ggctggaacg ctattttgcc gatcgtaatc tggggcagga aaattttgat
    22381 gctgcagaaa aagcggcacg cgaagtgtta cgtccggttg ccgatgaatt acggtatcac
    22441 ggctggaaag tgtgcgttgg tgcttccggc accgtgcagg cgttacagga aatcatgatg
    22501 gcacagggga tggatgaacg cattaccctg gaaaagttgc agcaattgaa acagcgagcc
    22561 attcattgcg gtcggctgga ggaactggag attgacgggc tgacgctgga acgtgcgtta
    22621 gtgttcccga gtggtctggc gatcctgatc gccattttta ccgaactgaa tattcagtgt
    22681 atgaccctgg cgggcggtgc gctgcgtgaa ggcctggtct acggcatgtt gcatctggcc
    22741 gtcgagcagg atattcgcag ccgtacgctg cgtaatattc aacgccgctt tatgatcgat
    22801 attgatcagg cacagcgcgt agccaaagtt gcggctaact tcttcgatca ggtggaaaac
    22861 gaatggcatc ttgaagcaat aagccgcgat ttgctcatca gcgcctgtca gcttcatgaa
    22921 atcggcctga gcgttgactt caaacaagcg ccgcaacacg ctgcttatct ggtgcgtaat
    22981 ctggatcttc ccggttttac ccccgcacag aaaaaattgc tggcgacgct actgctcaac
    23041 cagactaatc cggtcgatct ctcatcgctg catcagcaaa atgccgtacc gccgcgcgtc
    23101 gcagaacaac tctgccgttt actacgcctg gccatcattt ttgccagccg tcgccgtgac
    23161 gatctcgtgc cagagatgac attacaggct aaccatgaac tgttgacctt gacgcttccg
    23221 caaggttggc taacccaaca tccgctgggt aaagagatta ttgctcagga aagccagtgg
    23281 cagagctatg tccactggcc gctggaagtg cattaatgag taagtgccgg atgcgatgct
    23341 gacgcatctt ttccggcctt gattatttcc ctcgttttgc cgccatcatc gctttcagat
    23401 tcgccagatg gctttgccct ttctgcatcc gttcttcggc gctgaccact ttgcgctcct
    23461 gttcccaaat cagatcatcc tgcggcagct ccagcaaaaa gcggctcggc tccgggcgca
    23521 ccagttcgcc gtactgacgg cgttctttac acagcgtaaa ggtcaattcc ttctgggcgc
    23581 gggtaatgcc gacataggcc agccgccgct cctcatcgat attatcttca tcgatactgc
    23641 tctggtgcgg caaaaaccct tcttccatac cgaccatgta gacataagga aactccagtc
    23701 cttttgacgc gtggagagtc atcagttgca cctgatccag ctcttcttca ctctcaccac
    23761 gctccatcat gtcgcgcaaa gtaaagcgcg tcaccacctg ggtgagcgtc atcggctcat
    23821 ccagttcgct gccttccagc atctccgtca tccagctaaa cagttggttg acgttcttca
    23881 tgcgcatttc ggcggctttc gggctgggcg atgtttcgta cagccaggat tcataatcca
    23941 tgccatggat cagatcacgc accgcagcaa tcggctcccg ctccgccagg cgctggattt
    24001 ctgccaacca gtgagtgaag cgggtcaatg cttcataacc acgtccgcta agcgtctgac
    24061 tcaggcccat atcaaagctg gcggtaaaca tgcttttatt gcgcgtcatc gcccactcac
    24121 ccagcttttt cagcgtagcc gggccaatct ctcgcttcgg cgtgttaacg atacgcagaa
    24181 atgcgctgtc atcgtccggg ttagtcagca cgcgcagata agccagcaag tccttgattt
    24241 caggacgaga gaaaaacgac gtaccaccgg atattttgta cgggatgcgg ttttgcatca
    24301 ggaatttttc aaatacccgc gactgatggt tgccgcgata aagaatggcg taatctttgt
    24361 actgcgtttt attgacgaag tgatgggcga tcagctcgcc agtaacgcgc tcggcttcat
    24421 gttcctcgtt attcgcgctt aaaactttta gctctgtgcc gtaacccagt tcggagaaca
    24481 gacgcttttc aaagacgtgc gggttattgg cgatcaggat gttcgccgct ttcagaatac
    24541 gcccggaaga gcgatagttc tgctcaagct taatcacctt cagcgccgga aaatcctgac
    24601 tcagcagcac caggttttgc ggacgtgcac cgcgccagga gtagatcgac tggtcatcgt
    24661 cacccaccac ggtaaagcgc gcgcggctgc ccaccagcag tttcaccagt tcatactggc
    24721 tggtgttggt gtcctgatac tcatccacca gcaggtagcg aattttgttc tgccagcgtt
    24781 cgcgtacttc ttcattgcgt tgcagcagca acgtcggcaa taaaatcagg tcatcgaagt
    24841 cgagaacgtt acaggctttc aggtgtgcat catacagccc ataacaatgg gcaaaaatac
    24901 gatcccgctc gccctttgcc tctgccgccg cctgcgccgg agttttgaga tcgttcttcc
    24961 agttagagat ggtcgaaatc agttgttgca ggagaacttt gtcatcttca atcagcccct
    25021 cggtcaactc tttaagcaaa gcaagctgat cggtatcgtc aaataacgag aagttcgctt
    25081 tcatcccaag cgccgcatac tcgcgtttga tgatatccag ccccaacgta tggaaagtgg
    25141 agatcatcag cccacgcgcc tctttacgcc ccagcgtctg ccctacacgc tctttcatct
    25201 cgcgcgctgc tttattagta aaggtcaccg ccgcaatgtg ccgcgcctga taaccgcaac
    25261 cgcggatcag atgggcgatt ttattggtga taacacgagt tttaccggaa cccgcgcccg
    25321 ccagcaccag gcaggggccg gtaacgaatt cgacagcttg ttgttggccg gggtttagac
    25381 gcataggtgt attgctcaat cttcgaacgg ggggaggatt gtagcagaat cgcatttgaa
    25441 tgtgaccgcg tgtaagccgc taaccggttg gctgcggatg tttaaatcag agagagaaga
    25501 ccgaaatcgg agttcatcgt gaatccatta gacttagaaa atatcgggtc gtaacgttgt
    25561 gttattactt tttcagagtt tactaaggag aggtttactc gtggcgatta atttcagccc
    25621 taaggttggt gaaatactgg aatgcaactt tggtaactat cctgttagcc aaaatggacc
    25681 gttcagcacg acgtattacg atggccgcat tcctcctgaa atgataaaaa acaggttagt
    25741 tgtcgtgctc aacggaaaaa ttaatggcaa cgcattcatc gtcgttccat tatcaaccac
    25801 tcgcgaccat gacaagttaa aacgtggaat gcatgttgaa atagccagta atgttatcaa
    25861 tgacctgcaa ttctttgatc agcagattcg ttgggccaaa accgacctgg tacaacaagt
    25921 cagtcgaaac agattaaaca gagcaagaac ttacagaggg tatcttaatc aatgtttgcc
    25981 acatgagttg gttgcagaca tacagagggc ggtcatcaag tcaatcaatg ccatatcgct
    26041 cataaattga caataaaatc accatccccg ataatgactt tgtagcccat agaggcccta
    26101 agttttctcc ccttgaggga cctgcgaagc ccggattttc cgggcttttg ccatatatgt
    26161 accagcccct ccctgactga caattacgtt agcatcggcg gatcatgcta aaatcgccgc
    26221 ctgacaatta ttcactcaag gcacgatcat ggcaaaaaca gcagcagcac tgcatatcct
    26281 tgtaaaagaa gagaaactgg ctctggatct tctcgagcag attaagaacg gggccgattt
    26341 tggcaagctg gcgaagaaac actccatttg cccatcaggc aaacgcggcg gtgatttagg
    26401 tgaattccgc cagggtcaga tggttccggc gttcgataaa gttgtgttct cttgtccggt
    26461 actggagcca accggcccgc tgcacaccca gttcggttac cacatcatta aggtgctgta
    26521 ccgtaactaa tagcaaggcc ttctccggga gaaggccttg aactctgaat cgctattaac
    26581 ccgcaacagc aatacgtttc atatcagtca tgtagccgcg cagtttctta cctacctgct
    26641 caatcgcatg gctgcgaatc gcttcgttca aatcacgcag ttgcgcgtta tctaccgcac
    26701 cttccggaat agctttaccc aaatcgcccg gttgcagctc tgccataaac ggtttcagca
    26761 acggcacaca agcgtaagag aacagatagt taccgtactc agcggtatca gagataacca
    26821 cgttcatttc gtacagacgc ttacgggcga tggtgttggc aatcagcggc agttcgtgca
    26881 gtgattcata gtaagcagat tcttcgatga tgccggaatc aaccatggtt tcaaacgcca
    26941 gctcaacgcc cgctttcacc atcgcgatca tcagtacgcc tttatcgaag tactcctgct
    27001 cgccgatttt gccttcatac tgcggcgcgg tttcaaacgc ggttttgccg gtctcttcac
    27061 gccaggtcag cagtttctta tcatcgttgg cccagtccgc catcatgccg gaggagaatt
    27121 cgccggagat gatgtcgtcc atatgtttct ggaacagcgg tgccatgatc tctttcagct
    27181 gttctgacaa cgcgtaagcg cgcagtttcg ccgggttaga gagacggtcc atcatcaggg
    27241 tgatgccgcc ctgtttcagc gcttcggtga tggtttccca gccgaactga atcagttttt
    27301 ctgcgtatgc cggatcggtg ccttcttcca ccagcttgtc gaagcacagc agagagccgg
    27361 cctgcaacat accgcacaga atggtttgct cgcccatcag gtcagatttc acttccgcaa
    27421 cgaaagagga ttccagcacg cccgcacgat gaccaccggt tgccgccgcc catgctttag
    27481 cgatcgccat gccttcgcct ttcggatcgt tttccgggtg aacggcaatc agcgtcggta
    27541 cgccgaatcc acgtttgtac tcttcacgta cttcggtgcc tgggcatttc ggcgcaacca
    27601 ttactacggt gatgtcttta cggatctgct cgcccacttc gacgatgttg aaaccatgcg
    27661 agtaacccag cgccgcgccg tctttcatca gtggctgtac ggtgtgcact acatcagagt
    27721 gctgcttgtc cggcgtcagg ttaaccacca gatccgcctg tgggatcagt tcttcgtaag
    27781 tacccacttt aaaaccattt tcggtcgctt tacgccagga cgcgcgcttc tcggcaatcg
    27841 cttctttacg cagagcgtag gagatatcga gaccagaatc acgcatgttc aggccctggt
    27901 tcagaccctg tgcgccacag ccgacgatga ccactttttt accctgaagg tagctcgcgc
    27961 catcggcgaa ttcgtcgcgg cccataaagc gacatttgcc caactgtgcc agttgctggc
    28021 gcaggttcag tgtattgaag tagttagcca tggtgattcc tcgtgatgtt gtgcttctta
    28081 ttgttcggtt cgcgtttgca agattgaatt cactatatga caggaaattt attgcggaaa
    28141 ttgatatatt cacaacatca tattgcaatt tttgcaacgt caacatcgag ggctgtccct
    28201 gtggatttac gcgatctgaa aaccttcctg catctggcgg aaagccgcca ttttggccgc
    28261 agcgcgcggg cgatgcacgt tagcccatcc acgctctcac ggcagattca gcgcctggaa
    28321 gaagatctcg gtcagccgct gtttgtgcgc gataaccgca cggtgacgct gactgaagcg
    28381 ggcgaagaac tgcgcgtttt cgcccagcaa acgctgttgc agtatcagca gctgcgccac
    28441 accatcgatc agcaagggcc gtcgctctct ggcgaattac atatcttctg ctcggtgacc
    28501 gctgcctaca gccatctgcc gccgattctg gatcgcttcc gcgcggaaca cccgtcggtg
    28561 gagattaaac tcactactgg tgatgcggca gatgcaatgg aaaaagtggt cacgggagaa
    28621 gcggatctgg cgattgccgg gaaaccggag acgctacccg gcgctgtagc gttttcgatg
    28681 ctggagaatc tggcggtagt gctgattgcg cccgcgctgc cctgcccggt gcgtaatcag
    28741 gtctcggtag agaagccgga ctggtcaacg gtaccgttta tcatggccga tcaggggcca
    28801 gtacgccgcc gcattgaact gtggtttcga cgcaataaaa tcagcaaccc aatgatttac
    28861 gccacggttg gcgggcatga agcgatggta tcgatggtgg cgctcggctg tggtgtggcg
    28921 ctgttgccgg aagtggtgct ggaaaacagc ccggagcctg tacgcaaccg ggtaatgatt
    28981 ttagatcgca gcgatgagaa aacgccgttt gaactgggcg tgtgtgcaca aaaaaagcgg
    29041 ctgcatgagc cgctgattga ggcgttctgg aggattttgc cgaaccacaa atgacgttgt
    29101 cgcgcgagta ggcctgataa gcgaagcgct atcaggcatt tttccctaac ccgccaaaaa
    29161 gaacctgaac gccgggttat tggtttcgtc gtggcaatcg tagcccagct cattcagccg
    29221 ggtttcgaaa tccggttcat ggtcgccaag ttcgaacgcc gccagtacgc gcccgtagtc
    29281 ggtgccatgg ctgcgatagt ggaacaaaga aatgttccag tgcgtaccca gcgtgttgag
    29341 gaagcgcagc agcgcgcccg gtgattccgg gaattcgaag ctgtagaggc gttcctgcaa
    29401 cggatgtgac ggacgcccgc cgaccatata gcgcacatgc aacttcgcca tttcgtcgtc
    29461 ggagagatcc accacgctgt agccgccgtc gttgagcatc tgcaaaattt ctttgcgctc
    29521 ttcgaggccg cggctcaggc gcacgccgac aaagatgcag gcgtttttgg catcggcaaa
    29581 acggtagttg aactcggtga ccgatcgccc gccaagcagt tggcagaatt tgaggaagct
    29641 gcctttttct tccggaatgg tcaccgccaa caacgcttca cgctgttcgc ccagttcgca
    29701 gcgttccgag acgtagcgca ggccgtggaa gttcacgttg gcaccggaaa gaatatgcgc
    29761 cagccgttcg ccgcgaatgt tgtgctgggc gatatatttt ttcattcccg ccagcgccag
    29821 cgcgccagag ggttccgcca ccgcgcgcac atcttcgaat aaatccttca tcgccgcaca
    29881 gatcgcatca ctatcgacgg tgatgatatc gtcgagatac tcctggcaca aacggaaggt
    29941 ttcgtcgccg atgcgtttta ccgcaacgcc ttcagcaaac agccccacgc gcggcagatc
    30001 aaccggatga cccgcatcca gcgctgcttt caggcaggcg gagtcttccg cttctacggc
    30061 gattactttg atttgcggca tcagttgttt gatcagcacc gccacgccag cagccagacc
    30121 gccgccgccg actggcacaa atacgcggtc gagatgggcg tcctgctgga gcagttccag
    30181 cgccagcgtg ccttgcccgg caatcaccat cggatggtcg aacggcggca cccaggtgaa
    30241 cccctgctgc tgtgacagtt cgatcgcttt ggctttcgct tcatcaaagt tcgcgccgtg
    30301 gagcagcact tcgccgccga agccgcgcac cgcgtcgact ttgatgtcgg cggtggcggt
    30361 tggcataacg atcagggcct tcacgcctaa ccgcgcagaa gaaaacgcga cgccctgcgc
    30421 gtggttaccc gcagaagcag tgatcacgcc gtgcgctttt tgttcttccg tcaggcccgc
    30481 catcatggcg tatgcgccgc gcagcttaaa gctgtgcact ggctggcgat cttcgcgctt
    30541 caccagaatc acgttatcaa gacgagacga gagcttttcc attttttgta gcggcgtgac
    30601 ctgcgccgct tcgtagaccg gcgcgcgcag caccgctctt aaatattcag caccttccgg
    30661 ggcaccggac aggggttgcg agtcagccat tattaacccc ccagtttcga tttatcgcgc
    30721 accgcgcctt tgtcggcgct ggttgccagg ctggcataag cacgcagggc aaaggagatc
    30781 tgacgttcac gatttttcgg cgtccaggct ttgtcacccc gagcttcctg cgcttcacga
    30841 cgcgccgcca gttcggcatc gcttacctgt aactgaatgc cacggttcgg aatgtcgatg
    30901 gcgatcaggt cgccgtcttc aatcaggcca atgctgccgc cgcttgccgc ttccggtgag
    30961 acgtggccga tagaaaggcc agaggtgccg ccagagaaac gaccgtcggt gatcagcgca
    31021 caggctttac cgagccccat tgatttcagg aagctggttg ggtagagcat ttcctgcatc
    31081 cccggaccac cttttgggcc ttcatagcga attactacca catctccggc gacaacttta
    31141 ccgccgagaa tcgcttctac cgcgtcgtcc tggctttcgt acactttcgc cgggccggtg
    31201 aatttgagga tgctgtcatc gacgcccgca gttttaacga tgcagccgtt ttccgcgaaa
    31261 ttaccgtaga gcaccgccag gccgccgtct ttgctatagg cgtgttccag cgagcggata
    31321 cagccattgg cgcgatcgtc gtccagcgta tcccaacggc aatcttgcga gaatgcctgg
    31381 gtggtacgga tacccgcagg gccggcgcgg aacatatttt ttaccgcgtc atcctgggtc
    31441 agcataacgt cgtattgttc cagcgtttgc ggcaacgtca ggccaagtac gtttttcaca
    31501 tcacggttca gtaaccccgc acgatccagc tcgccgagaa taccgataac accaccagca
    31561 cggtgaacat cttccatatg gtatttttgg gtgctcggcg cgactttgca taactgcgga
    31621 accttgcggg aaagcttatc gatatcactc atggtgaagt cgatttccgc ttcctgcgcc
    31681 gccgccagca ggtgaagtac ggtgttagtc gatccaccca tcgcgatatc cagcgtcatg
    31741 gcattttcaa acgccgcttt gctagcaata ttgcggggca gtgcactttc gtcgttttgc
    31801 tcgtagtaac gtttggtcaa ttcaacaatg cgtttaccag cattgaggaa cagctgctta
    31861 cggtcagagt gggttgccag cagcgagccg ttgcccggct gcgacagacc cagcgcttcg
    31921 gtcaggcagt tcattgagtt agcggtaaac atcccggagc aggaaccgca ggttgggcat
    31981 gcggaacgtt caacctgatc gctctgggag tcagatactt tcgggtctgc gccctggatc
    32041 atcgcatcaa ccagatcgag cttgatgatc tgatcggaca gtttggtttt cccggcctcc
    32101 atcgggccgc cggaaacaaa gatcaccgga atattcagac gcagggaagc catcagcatc
    32161 cccggggtga ttttgtcaca gttagagatg cagaccatgg cgtcggcgca gtgggcgttg
    32221 accatatact caacggaatc agcgatcagt tcgcgagatg gcagtgaata aagcatcccc
    32281 ccgtggccca tggcaatccc atcatccacc gcaatggtgt tgaactcttt ggcaacgccg
    32341 ccagccgctt caatttgttc ggcgaccagt ttaccgagat cgcgcagatg gacgtgaccc
    32401 ggtacaaatt gggtgaacga gttcacaacc gcgataatcg gcttaccgaa atcggcgtcg
    32461 gtcattccgg tggcgcgcca cagcgcacga gcacccgcca tattacgacc atgagtggtg
    32521 gtggcggaac ggtacttagg catactttat ttactcccag tgtctgtctc gtaaatggga
    32581 cggtgcgtgc cgtcccattt tttgtattta ttgattaact tgatctaacc agccccattt
    32641 atcttcggtt tcgccggtaa agaggccgaa gaaggcttgc tgaatgcgtt tggtgaccgg
    32701 gccacaacgg ccttcgccaa cctgaatacc gtctacactg cgcactggcg tgatttctgc
    32761 cgccgtaccg gacataaaga cttcatccgc caggtacaag gattcgcgcg acagcacctg
    32821 ctcacgcact tcaattccca gctctttcgc cagtttgatg atggcatcac gggtaatacc
    32881 cggcagcgcg gaggaggtga acggtggggt gaacagcaca ccatctttca cttcaaacag
    32941 gttttcgcct gcgccttcag agatataacc gttcacatcc agcgcgatac cttcctgata
    33001 accgtggcgg cgcgcttcgc tacccaccag cagggaagag agatagttac caccggcttt
    33061 tgccgcagtt gggatggtgt ttggtgctgc gcggttccag gaggaaacca tcgcatcgat
    33121 cccctgctcc agcgcttctg cgcccagata cgctccccac gggaaagcgg cgataatcac
    33181 atcggttgag tatcccgctg gcgggtttac gcccatgcca acatcaccaa cgaagatcag
    33241 cggacggata taggcgctgg tgagattgtt tttgcggatc acgtcacggc aagcttccat
    33301 cagctcatca atgctctgcg aaaccgggaa gcgatagatt ttggcggagt catgcagacg
    33361 ctgcatatgc tcacgatggc ggaatacaac cggtcctttg tgcgagtcgt agcaacggat
    33421 gccttcaaaa accgaggtgc catagtgcag cgcgtgcgac atcacatgca ccttcgcgtc
    33481 ttcccagcga accatctccc cattgaacca aatgtaatca gctttcttcg tggtcatttt
    33541 tatattcctt ttgcgctcag gcgcggattt gttgtgatgt ggttgtgctc tggcagatgg
    33601 caacgtgtgc gacgtccacc agtttattta actgactaaa cagtaagtcg accgaccgtg
    33661 ggctggcaac ggtcaattcg atatttatat tttgtgcatc gctggcggcg gccatattca
    33721 ttgagcagac gtggaaacca cgatgacgca ccacgcgtaa aacacgttct aaggtttccg
    33781 gattgaagcg agccgataca ttgacctgat gttgcatcat gataatttct ccaacatttc
    33841 tgaattactg gcaccaggcg gcaccagcgg ccagacgttc tcaagttcgt cgattgagac
    33901 atgaagcagg tatggcccat cactgttcag catggtgtcg agtgccgctt caacctggtc
    33961 tttacgggtg atgtgttggc cagggatgcc gaaggcgctg gctaacttga ggaaatcggg
    34021 gttatcggta agggtggttt cgctgtatcg ttcctggaaa aacagttgct gccattgtcg
    34081 aaccatccct aaccgttggt tatcgagtaa gacgattttc aacggtaact gcttgcgttt
    34141 tacggtgccc agctcttgta cattcatcat gaaagagccg tcaccggaga tacagacgac
    34201 ggtatcgttt ggtcgcgcga cttgcgcgcc aaccgccgcc ggtaaaccaa aacccatggt
    34261 gcctaagccg ctggaggtaa tgaaattttc cgggcgagtg tgtgcgatgt gctgcgcggc
    34321 ccacatctga tgctgcccca catctgtggt cacaacgcaa tccgcaggtt tacgatccga
    34381 cagttgtttt aacaacaacg gcgcgtagat agcgtcaccg ggatggtcgt aacgccaggc
    34441 atgttcatca cgcagctgcg cgcagtattg ctgccagtca ttgatattta atggctgctg
    34501 taatgctggt aacagcgcat ttaaatcacc ctgtaatgcc acatgtgcct gacgcagctt
    34561 gttcatttct gccgggtcga tatccatatg gataacactg gcgtgtggtg cgaaggtgtt
    34621 caatttgccg gtcacccggt catcaaaacg tgcacccacg gcgatcagca agtcgcactc
    34681 ctgcaccgcg aagttcgccg ctttggtgcc atgcattccc agcatgccca gatagtacgg
    34741 ataatctgct tcaactgcgc ccagcccttt cagcgtgcag gtggcaggca tttttgtggt
    34801 agcgagaaat tctcgtaaag caggaactgc ctgcgccata cccacgccac caccaacgta
    34861 cagcatcggt ttttgcgctt ttgccagcat ctggcgcgct tgctcaactt cggcatgtgg
    34921 gaaagtcact tcgttttcaa cggtggtgaa ccacggttcc aggtcgccgc tggctaactg
    34981 gatatctttt gggatatcga ccagaaccgg accaggacga cctgagctgg caacgtcgaa
    35041 tgcttcagcc ataatgcgcg gcaactcttc cagcgactgc accagaaagc tgtgcttggt
    35101 acaggctaac gacaatccca ggacatccac ttcctgaaat gcgtcagtac cgataaacgg
    35161 tgcggacact tgaccggtga tggcaacaac agggatagaa tctaacagtg cgtccgcaag
    35221 cccggttatc aggttggttg cgcccggacc agacgtggcg atacatacgc cagttttgcc
    35281 ggtagcacgg gcataaccga tagccgccat tgccgcaccc tgctcatgtc ggcacagcaa
    35341 gtgctccacg ccgccgtcat acaatgcatc gtaaaccggc ataattgcgc cacccggata
    35401 accgaaaacg gtgttcacac cctgtgcccg caacgcatgt accacccact gtgcgccatt
    35461 catagttagt tccccgtcct gaatcttggg aaacagaatt ttgtgctgtt attcattgtc
    35521 tgctcctcgg ttatgttttt aaggtcaaaa aaaacccccg gacctttcgg tgcgggggtc
    35581 ttagttcgtt aaggcttgat ctctaagcct ttcctcgtcc aagtgcagcc ccgcacggtg
    35641 ggataataat caccaccacg ctaatcacga ccaggctaat cactcgtaga agggctgtca
    35701 ttttgtcttt tcttgcatct tgttcgaa
//
LOCUS       DBFIFM010000022        35059 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.022, whole genome shotgun sequence.
ACCESSION   DBFIFM010000022 DBFIFM010000000
VERSION     DBFIFM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.022"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            135..731
                     /locus_tag="N7C74_001007"
     CDS             135..731
                     /locus_tag="N7C74_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2430927.1"
                     /translation="MKKALLAAALVMASGSALAVDGGHIDFNGMVQSGTCKVGVVDTG
                     MHSVTTDGVVTLDTANVTDTFAEVSATAVGLLPKEFMISVECDPGAPKNAELTMGSAS
                     YANTSGTLNNNMNITVNGIAPAQNVNIAVHNMKNKAGAAEIKQVHMNNSSEVQELTLD
                     AEGKGQYVFNASYVKAPNSPAVTAGHVTTNALYTVAYK"
     gene            818..1543
                     /locus_tag="N7C74_001008"
     CDS             818..1543
                     /locus_tag="N7C74_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HGU2430928.1"
                     /translation="MFPLVKKTVSALFVSTLLASAPAFADIIISGTRIIYNADKKDVN
                     VRLENKGNRPLLIQNWLDTGDDNADPSQIKVPFTATPPVSRVEPKRGQTVKVMYTGAT
                     ALPKDRESVYWFNVLEVPPKPKDAEADKNLLQLAFRTRIKLFYRPSGLQGEPAEAPAK
                     ITWKLNNAQLQANNPTPYYVSFNEVKLESGGKTYNVNSSMVTPFSQASFGVTSLPGSV
                     SSGKVVFKAINDFGGNIDGSATF"
     gene            1585..4185
                     /locus_tag="N7C74_001009"
     CDS             1585..4185
                     /locus_tag="N7C74_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HGU2430929.1"
                     /translation="MYQFTHQKSRIPKKTLLAACCALFYSSNGAAADTVEYDSSFLMG
                     TGASTIDVKRYAQGNPTPPGLYNVRVFVNGQATSSLEIPFVDIGENSAAACLTHKNLA
                     QLHIKQPEQPVTLLAREGEEEDCLDLAKSYEKADVCFDGSDQFLDLTIPQAYVLKSYG
                     GYVDPSLWESGINAATLAYTLNAYHTSSDNDNSDSVYGAFNSGINLGAWHFRARGNYN
                     WTTDNGSDFDFQDRYLQRDIPAIRSQIIMGDAYTTGETFDSVNVRGVRLYSDSRMLPS
                     ALASYAPTIRGVANSNAKVTVTQSGYKIYETTVPPGEFVIDDISPSGFGSELVVTIEE
                     ADGSKRTFTQPFSSVVQMQRPGVGRWDFSAGKVIDDSLRSEPNMGQASYYYGLNNLFT
                     GYTGIQFTDNNYLAGLLGVGINTSIGAFAVDVTHSRAEIPDDKTYQGQSYRVTWNKLF
                     QDTGTSFNLAAYRYSTQDYLGLHDALVLIDDAKHLSADEDKNTMQTYSRMKNQFTVSI
                     NQPLNIAYEDYGSLFISGSWTYYWAANNSRTEYNVGYSKSVSWGSFSVNLQRSWNEDG
                     EKDDAMYVSVSVPIENILGGKRKSSGFRNLNTQLNTDFDGSHQLNVNSSGNTENNLVN
                     YSVNAGYSLDKNAGDLASVGGYLNYESGLGGISASASATSDNSQQYSISTDGGFVLHS
                     GGLTFTNNSFSSNDTLVLINALGAKGARINNSNNEIDRWGYAVTSSVSPYRENRVGLN
                     IETLENDVELKSTSATTVPRSGSVVLTRFETDEGRSAVLNITAANGKSIPFAAEVYQG
                     EVMIGSMGQGGQAFVRGINDSGELIVRWYENNQTIDCKLHYQFPAQPQTQGSTNTLLL
                     NNLTCQVANH"
     gene            4199..4747
                     /locus_tag="N7C74_001010"
     CDS             4199..4747
                     /locus_tag="N7C74_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2430930.1"
                     /translation="MKRILLTSALIGLGLPAVGSATDLNVDFTATVLATTCTITIVED
                     GGPAVTGSNDEYSLTIPDVGLDKVATAAPEAQANFKLKASDCSNGYSKIFTTLTGTTV
                     SGKLIVNEATSGAAGVGMGIKRRDTADSTFFTPNNTDKFEWSADEKASGVPLTVALRE
                     TTAGAGRTGAFQAKATFNFTYE"
     gene            4762..5367
                     /locus_tag="N7C74_001011"
     CDS             4762..5367
                     /locus_tag="N7C74_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2430931.1"
                     /translation="MKLKVIATLIATVAVGVSFNSNFASASTTSASLTVNSNLTMGTC
                     SAQIMDNSNKVINEVVFGNVYISELGAKSKVQQFKIRFSNCSGLPQNSAQIVLAPNGI
                     SCAGSQSSSAGFSNKFTDASAATRTAVEVWTTDTPESNGSTQFHCAQKIPVPVTLPAD
                     TTTQPYDYPLSARMTVAEGRLVTDVRPGNFRSPTTFTITYQ"
     gene            5388..5978
                     /locus_tag="N7C74_001012"
     CDS             5388..5978
                     /locus_tag="N7C74_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HGU2430932.1"
                     /translation="MLCRHHKIVHFLGLATALITPFAYSGQDVDLTAKIVPSTCQVEV
                     SNNGVVDLGTVTLDYFADNVTPTTDYAGGKTFNVNVVSCDNIQTTQSQMKLDFQPQAG
                     SLAQANNQIFSNEYEQQATGAKNVGIVIFSAQPNQQTFNVRGTDGSSTAIYSVAPGNA
                     VPSTWTFYSRMQRVNNALPPESGMVRSQVIVNVSYE"
     gene            5990..7099
                     /locus_tag="N7C74_001013"
     CDS             5990..7099
                     /locus_tag="N7C74_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2430933.1"
                     /translation="MQRKGNKLLIQLCSVILLFFTTSWYALANECYIERNAEGDYHMK
                     ISSTQLSLASQMVEVPTEIAEATWDVNIQLRGDAIGCKSLGDSKAVHFLNTADPSLIS
                     TYTTTNGAALLKTTVPGIVYSVELLCLSCGAADELDLWLPAQSGADNFIPSTQTKWAY
                     EYSDQSWYLRFRLFITPEFKPKNGVSSGTTIAGKIASWYIGTNDQPWINFYIDNDSLK
                     FFVDEPTCATVALAQDQGNVSGNQVTLGNSYVSEVKNGLTREIPFSIRAEYCYASKIT
                     VKLKAANKPSDATLVGKTTGSASGVAVKVNSTYDNSKVLLKADGSNTVDYNFAAWSNN
                     LLFLPFTAQLVPDGSGNAVGVGTFSGNATFSFTYE"
     gene            7234..8028
                     /gene="panB"
                     /locus_tag="N7C74_001014"
     CDS             7234..8028
                     /gene="panB"
                     /locus_tag="N7C74_001014"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HGU2430934.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHESTLPVTVEDIAYHTTAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8040..8891
                     /gene="panC"
                     /locus_tag="N7C74_001015"
     CDS             8040..8891
                     /gene="panC"
                     /locus_tag="N7C74_001015"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HGU2430935.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(9190..10086)
                     /gene="rpnC"
                     /locus_tag="N7C74_001016"
     CDS             complement(9190..10086)
                     /gene="rpnC"
                     /locus_tag="N7C74_001016"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HGU2430936.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSESQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            10360..10740
                     /gene="panD"
                     /locus_tag="N7C74_001017"
     CDS             10360..10740
                     /gene="panD"
                     /locus_tag="N7C74_001017"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HGU2430937.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10744..11973)
                     /gene="yadE"
                     /locus_tag="N7C74_001018"
     CDS             complement(10744..11973)
                     /gene="yadE"
                     /locus_tag="N7C74_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGU2430938.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFDKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     EPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12037..12477)
                     /gene="yadI"
                     /locus_tag="N7C74_001019"
     CDS             complement(12037..12477)
                     /gene="yadI"
                     /locus_tag="N7C74_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGU2430939.1"
                     /translation="MLGWVITCHDDRAQEILDVLEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSIWHQQQKNPPFVLKHNLYEY"
     gene            complement(12582..13352)
                     /gene="yadH"
                     /locus_tag="N7C74_001020"
     CDS             complement(12582..13352)
                     /gene="yadH"
                     /locus_tag="N7C74_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2430940.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13349..14275)
                     /gene="yadG"
                     /locus_tag="N7C74_001021"
     CDS             complement(13349..14275)
                     /gene="yadG"
                     /locus_tag="N7C74_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2430941.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14384..15046
                     /gene="can"
                     /locus_tag="N7C74_001022"
     CDS             14384..15046
                     /gene="can"
                     /locus_tag="N7C74_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HGU2430942.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLDEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15087..15623)
                     /gene="hpt"
                     /locus_tag="N7C74_001023"
     CDS             complement(15087..15623)
                     /gene="hpt"
                     /locus_tag="N7C74_001023"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HGU2430943.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15829..18219
                     /gene="gcd"
                     /locus_tag="N7C74_001024"
     CDS             15829..18219
                     /gene="gcd"
                     /locus_tag="N7C74_001024"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HGU2430944.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18421..19971)
                     /gene="cueO"
                     /locus_tag="N7C74_001025"
     CDS             complement(18421..19971)
                     /gene="cueO"
                     /locus_tag="N7C74_001025"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HGU2430945.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKERAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20137..20484
                     /gene="yacC"
                     /locus_tag="N7C74_001026"
     CDS             20137..20484
                     /gene="yacC"
                     /locus_tag="N7C74_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HGU2430946.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20590..21456
                     /gene="speE"
                     /locus_tag="N7C74_001027"
     CDS             20590..21456
                     /gene="speE"
                     /locus_tag="N7C74_001027"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HGU2430947.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            21472..22266
                     /gene="speD"
                     /locus_tag="N7C74_001028"
     CDS             21472..22266
                     /gene="speD"
                     /locus_tag="N7C74_001028"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HGU2430948.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22304..22666)
                     /gene="yacL"
                     /locus_tag="N7C74_001029"
     CDS             complement(22304..22666)
                     /gene="yacL"
                     /locus_tag="N7C74_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HGU2430949.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22842..25439)
                     /gene="acnB"
                     /locus_tag="N7C74_001030"
     CDS             complement(22842..25439)
                     /gene="acnB"
                     /locus_tag="N7C74_001030"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HGU2430950.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25794..27647
                     /gene="yacH"
                     /locus_tag="N7C74_001031"
     CDS             25794..27647
                     /gene="yacH"
                     /locus_tag="N7C74_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HGU2430951.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKTVPVTQTVTAPVIPSN
                     TVSTANPVITEPATTVISIEPGNPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDNYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVHEKMQTNPQNQQRREAARERIQPASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFREKAQESRPQRLNDSNHTARLNNEQRSAVRERLSERGARRLER"
     gene            complement(27718..29142)
                     /gene="lpdA"
                     /locus_tag="N7C74_001032"
     CDS             complement(27718..29142)
                     /gene="lpdA"
                     /locus_tag="N7C74_001032"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HGU2430952.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29350..31242)
                     /gene="aceF"
                     /locus_tag="N7C74_001033"
     CDS             complement(29350..31242)
                     /gene="aceF"
                     /locus_tag="N7C74_001033"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HGU2430953.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAVPEA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNTGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31257..33920)
                     /gene="aceE"
                     /locus_tag="N7C74_001034"
     CDS             complement(31257..33920)
                     /gene="aceE"
                     /locus_tag="N7C74_001034"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HGU2430954.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(34081..34845)
                     /gene="pdhR"
                     /locus_tag="N7C74_001035"
     CDS             complement(34081..34845)
                     /gene="pdhR"
                     /locus_tag="N7C74_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HGU2430955.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         8675 a         9255 c         8585 g         8544 t
ORIGIN      
        1 aatagcaaca attcaataca ccacgtaaat atattcacac ccgtgataat tcacccctat
       61 tacatcctcc aaaaactttg agtaatatcc gctcgcaata ttaatttcat ataatttttg
      121 taaggatgca tgtaatgaaa aaagcacttc tcgcagccgc tctggttatg gcttctggtt
      181 ccgccctggc tgtagatggt ggtcatatcg actttaacgg tatggtacag tccggtacct
      241 gtaaagtggg tgtggtagat actggtatgc atagcgttac cactgatggc gtggttaccc
      301 tggatactgc gaatgttact gatacttttg ctgaagttag cgcaactgct gtcggtttac
      361 tgccgaaaga gttcatgatt tctgttgagt gtgatccagg tgctccgaag aatgctgagt
      421 taactatggg ttctgcaagt tacgcgaaca ccagcggtac cctgaataac aatatgaaca
      481 tcactgttaa cggtattgca ccggctcaga acgtaaacat tgcagttcat aacatgaaaa
      541 acaaagctgg cgctgctgaa attaagcagg tccatatgaa caactcttct gaagttcagg
      601 aactgacatt agacgcagaa ggtaaaggcc agtacgtatt taacgcatct tacgttaaag
      661 caccgaacag cccggctgta actgctggtc atgtaaccac taacgcgctg tacaccgttg
      721 cttataagta attgatggtg tgttgagcat aaggtttgcc agcaggcaaa ccttattttc
      781 gcgaatttaa tctttctaca gacagcaagg agtgcttatg tttcccttgg ttaaaaaaac
      841 ggttagtgca ttatttgtga gtactctgtt agccagtgca ccagcattcg ctgacattat
      901 tatttccgga acacgtatca tttataacgc tgataaaaaa gatgttaacg ttcgtctgga
      961 aaataaaggg aatcgcccct tactgattca gaactggttg gatacgggcg atgacaatgc
     1021 cgatcccagc cagatcaaag tgccgtttac cgcgacccca ccagtttcac gcgtggagcc
     1081 aaaacgcggt caaaccgtaa aagtgatgta taccggtgca acagccctgc cgaaagaccg
     1141 tgaaagcgtc tactggttta acgtgctgga agtgccgcct aagccgaaag atgctgaggc
     1201 tgacaaaaac ttgttacagc tggctttccg cacacgcatc aaactctttt atcgcccgag
     1261 tgggttacag ggagaaccgg ctgaagcacc ggcgaaaatc acctggaaat taaacaacgc
     1321 tcagttacag gcaaataacc caacgccata ttacgtgtcc tttaatgagg tcaagctgga
     1381 aagcggcggc aaaacctaca acgtcaattc cagcatggtc acgccgttca gccaggcaag
     1441 ctttggcgta accagtctgc caggaagcgt gtcatccgga aaagtggtct ttaaagcgat
     1501 caacgacttt ggcggcaaca ttgatggttc cgcaacattc taaaaaataa acggattctt
     1561 tttaacatcg ccaggaatgc aaccttgtac cagtttactc atcaaaaaag ccgtatcccg
     1621 aaaaaaacgc tacttgcggc ctgttgtgcc ctgttttata gcagcaacgg tgctgcggcg
     1681 gacaccgtgg aatatgacag ttccttttta atgggaactg gcgcatcaac gattgatgtt
     1741 aaacgttatg ctcaaggcaa cccgacaccg ccgggtctct ataatgtccg cgtatttgta
     1801 aacggtcagg cgacttccag cttagaaatt ccgtttgtgg atattggcga aaacagtgcg
     1861 gcggcctgtc ttacccataa aaacctggcg caacttcaca ttaagcaacc tgaacagcct
     1921 gtcactttac tcgccagaga aggtgaagaa gaggattgtc tggatctggc aaagtcatac
     1981 gaaaaggcgg atgtgtgctt tgacggtagt gaccagtttc tcgatctgac gatccctcag
     2041 gcctatgttc tgaaaagcta tggcggctac gttgaccctt ctttatggga atcgggaatt
     2101 aacgctgcca cactggcata taccctgaac gcgtatcaca caagttcaga taacgacaat
     2161 agtgacagcg tctatggcgc gttcaactca ggtatcaatt taggagcctg gcactttcgt
     2221 gcgcgcggta actataactg gacaacagat aacggcagcg atttcgattt ccaggatcgt
     2281 tacttacagc gtgacattcc ggcaatccgt tcccagataa ttatgggtga tgcctatacc
     2341 accggtgaaa cgtttgactc tgtcaacgtc cgtggtgttc gcctgtacag cgacagccgt
     2401 atgctgcctt cggcgctggc cagttacgct ccgaccatcc gcggtgtagc aaactccaac
     2461 gccaaagtca ccgtgacgca aagcggatat aaaatttatg aaaccaccgt tccgcccggt
     2521 gaatttgtta tagacgacat tagcccttcc ggctttggta gcgaactggt cgtgaccatt
     2581 gaagaagcgg atggttccaa acgcaccttt acgcaaccct tctcgtcggt tgtacaaatg
     2641 caacgtcctg gtgtgggccg ttgggatttc agcgcgggta aagtcattga tgacagtctg
     2701 cgatccgaac ccaatatggg gcaagcctct tattactatg gtctgaataa cctcttcacg
     2761 ggttataccg gcattcagtt caccgataat aactatcttg ccgggctgtt aggtgtgggt
     2821 atcaacacca gcatcggcgc ctttgcggta gacgttaccc attcccgtgc tgaaattccg
     2881 gatgataaaa cctaccaggg gcaaagttat cgcgtgacct ggaacaaact tttccaggat
     2941 accgggacat catttaacct cgcggcgtac cgctattcca cccaggatta cctgggcctg
     3001 catgatgcgt tagtcctcat tgacgacgcc aagcatttgt ctgccgatga agacaaaaac
     3061 accatgcaga cgtactcacg tatgaaaaac cagtttaccg tcagcattaa ccagccattg
     3121 aatatcgcct atgaagatta cggttcgctg tttatttccg gtagctggac gtattactgg
     3181 gcggcgaaca atagccgcac tgaatataat gttggttaca gtaaaagcgt ttcgtggggc
     3241 agtttcagcg tcaacctaca acgtagctgg aatgaagacg gcgagaaaga tgacgcgatg
     3301 tacgtcagcg ttagcgtacc tattgagaat attttaggtg gcaaacgtaa gtcttctggt
     3361 ttccgcaatt taaatactca gctcaatacc gatttcgatg gttcacatca gttgaatgtt
     3421 aacagttccg gtaacactga aaacaatctg gtgaactaca gtgtcaacgc aggttatagc
     3481 ctcgataaaa acgccggcga tttagcctct gttggtggtt atctcaacta tgaatctggg
     3541 ttaggcggta tttccgcttc ggcctcggcc acttctgata acagccaaca gtactccatc
     3601 tcaaccgatg gcggctttgt attacacagt ggtggtttaa cgttcactaa caacagtttc
     3661 agcagtaacg acacgctggt gttaatcaac gccctaggtg ctaaaggcgc acgaatcaat
     3721 aacagtaata acgaaatcga tcgctgggga tatgccgtga cgtcctctgt cagcccatat
     3781 cgtgaaaacc gggtaggtct gaacattgaa acactggaaa acgatgttga actgaaaagt
     3841 accagcgcca ccaccgtacc acgtagcggc tccgttgttt tgacccgttt cgaaactgac
     3901 gaggggcgtt ctgccgtgct gaatattact gccgccaatg gcaaatccat tccgtttgct
     3961 gcggaggttt accagggtga ggtgatgatc ggcagcatgg gccagggtgg tcaggcattt
     4021 gtacgcggta ttaacgacag cggggaatta atcgtgcgct ggtatgaaaa caaccaaacc
     4081 attgactgta agttgcacta ccagttcccg gcgcagccac aaacgcaggg aagcaccaac
     4141 accttattac ttaacaatct tacctgtcag gtagcaaatc actaatatgg aaaattctat
     4201 gaaaaggata ctactaacat ccgcgttaat aggcctgggt ttaccggcgg ttggctctgc
     4261 aacagatctt aatgtcgatt ttacggccac cgtattagcc accacctgca cgataactat
     4321 agtggaagat ggtggtccag cagtgaccgg tagtaacgat gaatacagcc tgacaattcc
     4381 tgatgttggt ctggataaag tcgctaccgc tgcgcccgaa gctcaggcca actttaaatt
     4441 aaaggcctcc gattgcagca atggttactc aaagattttt accacactta caggaacaac
     4501 ggtttccggt aagttaattg ttaatgaagc aacctctggc gctgctgggg tggggatggg
     4561 gattaagcgt agagataccg cagactcgac tttcttcaca cccaataata ctgacaaatt
     4621 tgaatggagc gccgatgaga aagccagcgg tgtgcctctg accgtagcac tgcgcgaaac
     4681 aacggcggga gcaggacgca ccggtgcgtt tcaggcaaaa gccaccttta acttcaccta
     4741 cgaataacag ggacagccac catgaaatta aaagtcatcg ctacactgat tgctactgtt
     4801 gccgtgggtg taagctttaa cagcaatttt gcttctgcga gtacaacgtc cgcttcttta
     4861 accgtaaaca gtaacctgac tatgggtacc tgcagtgctc agataatgga taatagtaat
     4921 aaagtgatca atgaagtggt ctttggcaat gtttatattt ctgaactcgg tgcaaaaagc
     4981 aaagtgcaac agtttaaaat tcgctttagc aattgctctg gccttcccca aaacagcgcc
     5041 caaatagtgc tggcacctaa tggtatatcc tgtgctggtt ctcaatcgtc atcggcgggt
     5101 ttttctaaca agtttactga cgctagcgca gcaaccagaa cggctgtgga agtatggact
     5161 acagatacac cggaaagcaa tggcagtacg caattccatt gtgctcaaaa gataccagtg
     5221 cctgtgacgc ttcccgccga caccacaact cagccttacg attacccgtt aagtgcacgg
     5281 atgaccgttg cggaaggtag attggtaacc gatgtaagac cgggtaattt ccgctctccc
     5341 acgactttca cgatcactta tcagtaatag cggataagga tattagaatg ctatgcaggc
     5401 atcataaaat cgtacacttt ttagggctgg caacagccct tatcacgcct tttgcctatt
     5461 ccggccagga tgtggatctc accgcaaaga tcgtgcccag cacctgtcag gttgaagtca
     5521 gtaataatgg cgttgtcgat ctcggcacag tgacgctgga ctattttgcc gacaatgtaa
     5581 ccccgacaac ggattatgcg ggtggcaaaa cgtttaatgt gaatgtggtc tcttgcgata
     5641 acattcaaac gacacagtcg caaatgaaat tagattttca gccgcaggcg ggatcgcttg
     5701 cacaggcgaa taatcaaatt tttagtaatg aatatgaaca gcaggctacc ggggcgaaaa
     5761 acgtcggcat tgtgattttt tcggcccaac cgaatcagca gacatttaac gtgcgaggaa
     5821 cggatggttc ctcaacagca atttattcgg tagcacctgg caatgctgtt ccctcaacct
     5881 ggacctttta ttcccgtatg cagcgggtga ataatgccct tcctcctgaa tcgggaatgg
     5941 tgagaagcca ggttattgtt aacgtatctt acgaataata aggataatta tgcaaaggaa
     6001 aggcaataaa ctgttgattc agttatgcag tgtgatactg ctatttttta ccacatcctg
     6061 gtatgcattg gcgaatgaat gttatataga gagaaatgct gaaggggatt atcacatgaa
     6121 gataagctct actcagctta gtctggcgtc acaaatggtc gaggttccga cagaaatagc
     6181 cgaagctaca tgggatgtaa atattcaact aagaggcgat gccatagggt gtaaatctct
     6241 tggggatagt aaggcagttc actttcttaa tacagctgac ccaagtttaa tatccacgta
     6301 caccacaacg aatggcgcag cgttattaaa aacaactgtt ccaggcattg tgtattctgt
     6361 cgagttatta tgccttagtt gtggtgccgc agatgaactt gatttatggc tacctgcaca
     6421 aagtggcgca gataacttca taccaagcac ccagacgaaa tgggcctatg agtacagtga
     6481 tcaaagttgg tatttacgtt ttcgcttatt cataactcct gaatttaaac ccaagaatgg
     6541 tgtttccagc ggaacaacga tagcaggaaa gattgcgtca tggtatatag gtaccaatga
     6601 ccagccgtgg atcaactttt acattgacaa tgactcttta aagtttttcg tcgatgaacc
     6661 gacctgtgca acagttgccc tggcacaaga tcagggcaac gtcagtggca atcaggtaac
     6721 gcttgggaac agctatgttt cggaagtgaa aaatgggctt acgcgggaaa tccctttttc
     6781 tatccgtgct gaatactgtt atgccagtaa aattacggtt aagttgaaag cggcaaataa
     6841 acccagcgat gccacactgg tgggtaaaac gactggctcg gcttcaggcg tggctgtaaa
     6901 agtaaattca acttatgaca atagcaaagt attgttaaaa gcagatggta gcaacacggt
     6961 tgactacaac ttcgccgcct ggtcaaacaa cctgctgttt ttacctttta cggcgcagct
     7021 ggtaccggat ggtagcggta atgctgtcgg tgttggaaca ttttcaggta acgcgacctt
     7081 ctcctttacc tacgaataag taaaacgtca ttccctcctc atcgcaccag agttctgctc
     7141 tggtgcgaac atttccccac actaataacc attgcctccc tgttttcggc tgttagaatg
     7201 cgatcaatct gactttagcc atcaggatac gttatgaaac cgaccaccat tgcttcattg
     7261 cagaaatgta agcaggaaaa aaaacgcttc gccaccatca ccgcctacga ctacagcttc
     7321 gcaaaactgt ttgccgagga agggctgaac gtgatgctgg tgggcgactc gctggggatg
     7381 accgttcagg ggcatgaatc tacccttccc gttaccgttg aagatatcgc ctaccacact
     7441 accgcagtac gtcgtggcgc gccaaactgc ctgctgttgg ctgacctgcc gtttatggcg
     7501 tatgccacgc cggaacaagc ctttgaaaac gccgcaacgg ttatgcgtgc cggtgctaac
     7561 atggtcaaaa ttgaaggcgg tgagtggctg gtcgaaaccg taaaaatgct gaccgaacgt
     7621 gccgttcctg tatgtggtca cttaggttta accccacagt cagtgaatat tttcggtggc
     7681 tacaaagttc aggggcgcgg cgatgaagcg agcgatcgac tgcttagcga tgcattagcc
     7741 ttagaagccg ctggtgcaca gttgctggtg ctggaatgcg tgccggttga actggcaaaa
     7801 cgtattaccg aagcgctggc gatcccggtt atcggtattg gcgcaggcaa cgttaccgat
     7861 gggcagatcc tcgtgatgca cgatgcgttc ggcattaccg gcggtcatat ccctaaattt
     7921 gccaaaaatt tcctcgctga aacgggcgac atccgcgctg cggtgcggca gtatatggct
     7981 gaagtggagt ccggcgttta tccgggcgaa gaacacagtt tccattaagg agtgacgttg
     8041 tgttaattat cgaaaccctg ccactgctgc gccagcaaat tcgccgcctg cgtatggaag
     8101 gcaagcgcgt ggcgctggta cctaccatgg gcaacctgca tgatggccac atgaagctgg
     8161 tcgacgaagc caaagcccgc gccgatgtgg tcgtcgtcag tattttcgtt aacccgatgc
     8221 agttcgaccg cccggaggac ctggctcggt acccacgcac cttgcaggaa gactgcgaaa
     8281 agctgaacaa gcgcaaagtg gatctggttt tcgcgccctc ggtaaaagag atctacccga
     8341 acggtactga aacccatact tacgttgacg ttccagggct ttcgaccatg ctggaaggcg
     8401 ccagccggcc ggggcatttt cgcggcgtgt cgactatcgt cagcaagctg tttaacctgg
     8461 tccagccgga catcgcctgc ttcggtgaaa aggactttca gcaactggcg ctgatccgca
     8521 aaatggttgc tgatatgggc tttgatattg agattgtcgg tgtgccaatt atgcgcgcca
     8581 aagacggtct ggcactgagt tcccgtaacg gttatctgac ggcagaacaa cgcaaaattg
     8641 cgcccggtct gtacaaagtt ttaagttcga ttgccgacaa attgcaggct ggcgaacggg
     8701 atctcgatga aattattgct attgccgggc aagaactgaa tgaaaaaggc ttccgcgccg
     8761 atgatattca gattcgcgat gccgacacat tgctggaagt ctctgaaacc agcaaacggg
     8821 cagtaattct ggtagccgcc tggcttggcg atgctcgcct gatcgacaac aaaatggtcg
     8881 agctggcgta atacttatac tctaaataat tcaagttgca agttgcagga aggcggcaag
     8941 cgagtgacaa attcgtcagg aacgaatttg cccagccgca ggctggcctt cggtgagaga
     9001 catggatgtc tctcattaat ccccaggagc ttacataagt aagtgactgg ggttagcgaa
     9061 cgcagacgca gcacatgcaa tttgaagtat gacgagtata actggcgcta cggctgatgg
     9121 cgccaattat taatttgccc cacgtcatgg cgatggcgtc tgtaagcaat tattcataac
     9181 gcagacaact tagttaatca gcctatcaat ctcagcaaga gataaatttg tcatctctgc
     9241 aacgtcttcc cgagacatcc ctttactcag aaaacgctgg gcgaattctt gcctctcctc
     9301 ctgtcttccc tgctgaatcc ccttctcaat ccctttctct tcaaaccact gtgccagcgt
     9361 catcatagac ttccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     9421 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac tttcgctagt
     9481 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     9541 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     9601 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gcgccagctc
     9661 cggcgagtaa aacatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     9721 ataaaagaga atcggcacta ccagcggtag tttgtcgtga tccgcctcca gatgccgatg
     9781 catggcggct atagaataac gcatcatgcg aaaggccatt ttcttatccg gcttgctttg
     9841 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     9901 gtccgtgctg tgtcctttaa ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     9961 gttgaggtca caaagttcgc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
    10021 cgtctccgca tgcattaaaa attgtttaaa taccgcgtca tgcggtgtgg tactcggtgc
    10081 atccatcttc cctgtcttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgtgc
    10141 tatcgtcagc cacctcttta ttgtttaccg agcagcgttc agagaaatat caattcgtta
    10201 caggcgatac atggcacgct tcggcgcggt acgcaaactg ctgatgcaaa tgccgtcaga
    10261 tgagtagaca ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcac
    10321 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
    10381 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
    10441 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat tgatatctgg
    10501 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
    10561 attatttctg ttaacggtgc ggcggcccac tgcgccagtg tcggcgatat tgtcatcatc
    10621 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat
    10681 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
    10741 ttgttatccc tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct
    10801 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
    10861 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
    10921 gccgccaaac ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcgacg
    10981 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
    11041 ataaccatct acccgatgca aaaaatgggt atgtgactgg aaatcaaata catcgcgaat
    11101 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt ggttcccact tctgcgggtg
    11161 acgtttgatg cgcgaggtaa caataaacgc cgtcgccttc atgccatatt gtttcaacac
    11221 aggatacgca tagcggctca ctgacttgag gccatcatca aaggtaatca ccaccgctcg
    11281 cgcaggaaga ttgatcttat tcttcacgta accttccagc tgcaccatgc tcagtgtcgc
    11341 gtatcccctg tcgcgcagcc aggccatctg gttattgaaa gcgcgtaccg aggtggtcgt
    11401 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aggttagcac
    11461 cggaaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
    11521 acggatctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
    11581 caaattgtcc gccagtaccc caaatggcgc actgcccaca ctcggcgcgt tatagaccgg
    11641 cgtatctttc caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc
    11701 gtcttcaact ttttgtcgcc cctgaaccgg ctcaagatga cctttatcga taaacccttt
    11761 atcaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
    11821 tccggcccga atattcccca cggtcaccat cttgtcacca atttgcgccc agaccgcagc
    11881 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
    11941 catcagcagg agaataacag cttgtttgta catgaaaaaa tcaccgaggg tgaaacaacg
    12001 cttttattgt agcaaaagcg ccatcagaat cgcgggttaa tactcataca aattatgttt
    12061 gaggacgaaa ggcggatttt tttgttgctg gtgccaaata ctactcacct ccggcgcacc
    12121 cagttcgaca atacgctcgc gaaactctga actggtcatg gtttcacggc aagccatcat
    12181 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
    12241 taataatgaa gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc
    12301 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagaact
    12361 taatccgcgc cagaaattca cggcccggca ctgaagaagc gccccatgtt ttttctccag
    12421 cacatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
    12481 cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt
    12541 caaaactcct ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt
    12601 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
    12661 ccaaaggtag tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg
    12721 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
    12781 agtgagtaaa agaccccacc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
    12841 ctgatgtcat cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag ggagaacaac
    12901 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
    12961 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
    13021 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
    13081 ttacgctgga acttggcacc aaaaaatgat gacgcgacgt tggcgtaggc gttggtgatc
    13141 accgacatca tgatcagccc cggtacgatg aactgcatgt agctgaagcc atgcatatcg
    13201 ccaatacgcg aaccaatcag gttaccgaaa ataataaagt aaagggtcat ggtgatgact
    13261 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
    13321 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
    13381 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
    13441 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgcagc acttcaactt
    13501 ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
    13561 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
    13621 aggtattttc caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg
    13681 cttcttccag gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca
    13741 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcagtcggc tcgtcgagaa
    13801 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
    13861 cagataacat gcgcgcacgt tcgttgcgtt ttccccataa atcgagctgc ttaagatatt
    13921 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
    13981 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
    14041 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
    14101 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
    14161 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
    14221 gaacgccgcc tggatagttt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
    14281 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
    14341 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
    14401 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
    14461 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
    14521 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
    14581 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
    14641 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
    14701 cgcagttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
    14761 ctggttcaaa catagctcat tgctcgacga aatgccgcaa gagcgccgtc tggatacctt
    14821 gtgtgaactg aacgtcatgg aacaggtgta taacctgggc cactccacca ttatgcaatc
    14881 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcttatggca ttcacgacgg
    14941 cttgctgcgt gatctggatg ttactgccac caaccgcgaa acccttgagc aacgttaccg
    15001 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
    15061 atgcaacaca tccggcaact tcacacttac tcgtccagca gaatcacttt gccgatatac
    15121 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
    15181 atcgagaaac cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc
    15241 agcgtacaaa tcgccaacga cttcggttcg cgcaggctta agatctcacg cactttcgac
    15301 agtgtattcc ccgagtcgat gatatcttca acaatcagca catccttgcc acggatatct
    15361 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
    15421 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
    15481 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
    15541 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
    15601 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
    15661 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag caaaacgatt
    15721 cattcgcgga atctgttaat aaagctcaaa ttgtgatgac gatcacacat gttaaaacct
    15781 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
    15841 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
    15901 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
    15961 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggcgcagtaa acgtgccgcg
    16021 ctttggctat acgccgccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
    16081 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
    16141 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
    16201 gtggtggcac tgctgattag cggtagtatc ctgacctggg ccggatttaa cgatccgcag
    16261 gaaatcaacg gcaccttaag cgccgatgcc acacctgcgg aagctatctc acccgttgcc
    16321 gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa
    16381 caaattaacg ccgataacgt ccataatctg aaagaagcct gggtgttccg tactggcgat
    16441 gtgaagcagc cgaacgatcc gggcgaaatc accaatgaag tgacgccgat taaagtgggc
    16501 gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc
    16561 aaagagaaat ggcattacga tcctgagctg aaaactaacg agtctttcca gcacgtaacc
    16621 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
    16681 gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gcctgattgc gattaacgct
    16741 gaaaacggca agctgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
    16801 atgccagaca ccaaaccggg tctgtatgaa ccgacttcgc caccgattat caccgataaa
    16861 accatcgtga tggcaggttc agttaccgat aacttctcaa cccgcgaaac gtctggcgtg
    16921 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc gggcgcgaaa
    16981 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcgtgg
    17041 gcaccagcgg cctatgacgc gaagctggat ctggtgtatc tgccgatggg cgtgaccacg
    17101 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    17161 gcgctgaatg ccacaaccgg gaaattggcg tggagctacc agaccgttca ccacgacctg
    17221 tgggatatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    17281 gtgccagtta tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgtaat
    17341 ggcgaactgg tggttccggc accggaaaaa ccggttcccc aaggtgctgc caaaggcgat
    17401 tacgttaccc ctactcaacc attctcggaa ctgagcttcc gtccgacgaa agatttgagc
    17461 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    17521 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggcacgct ggtcttcccg
    17581 ggtaacctgg gaatgtttga atggggcggg atttccgttg atccaaatcg tgaagtggca
    17641 attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg tccgggtaac
    17701 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    17761 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccatttgg tctgccatgt
    17821 aaacagccag catggggtta tatctcggcg ctggatctga aaactaatga agtggtgtgg
    17881 aagaaacgta ttggtacgcc gcaggacagt atgccgttcc cgatgcctgt accggtgccg
    17941 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    18001 atcgccgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    18061 tggcagggtc gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggt
    18121 aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggtgac
    18181 tatattgtgg cttatgcgct gccggatgat gtgaagtaag acttgctcag attgctgaca
    18241 acgtgcgcgt tgttcatgcc ggatgcggcg tgaacgcctt atccagccta caaaattgtg
    18301 caaaatcaat ggattgcaca ggtaacgtag gcctgataag cgtagcgcat caggcagatt
    18361 tgcgtttgtc atcagtttaa tgcccggaga gatccgggca tattcccgaa tacggtcttt
    18421 ttataccgta aaccctaaca tcatccccgt atcttcatgc tccagcagat ggcagtgcgc
    18481 catataagca cgttctttcg gtgcgtcgtg attaaacttc accagcactt cactgacatt
    18541 gccttctacc ttaacggtat ctttccagcc cgcgcgatga gccgctggcg gtttgccatt
    18601 ttctgacaag atacggaact gcgtgccgtg aatatggaac ggatgcagca tcatgtcgcc
    18661 cacgccagag ataacccaac gttcgtactg ccctttcgcc gccgcaaaca tcggcttatt
    18721 catatcaaac gcctgaccgt tgattttatt ggcatggtgg aaatcgaact tcccgccgtg
    18781 gttcatatga ttcatattgc cgtgccccat atggcccatc atctggctgt gatccattcc
    18841 ggccatcgcc tgatcgccat atttctccat tagcatctgc atccccatca tatcgagcat
    18901 cgggtccata gaaagttgca gcttgcgtac cgtcagccct tccagcgaag gtaacgcagg
    18961 caggctactt aatgtgtctg gcaaagcacc ggaagcacta atagcaatcg gctgaatccg
    19021 cattaccgga tgaggcttat caaacggcgc aatcgccatc cccatctggc tgaccggcag
    19081 cgtcaccagg tcaaagggtt tgttgtcgtt aacctccacc agcacttcaa aacgctcgcc
    19141 catcagcacc ggcagctcgt tcaccttcac cggttcaggt agcagaccac cgtcgctggc
    19201 aatcacataa agcgggcgat tgtcgctggt ggcgaaattg agcgagcggg cattacagcc
    19261 attgagcaaa cgcaggcgca gccaaccacg cggggcagcg tgttgcgggt agattgcacc
    19321 gttggtcagc aacgtatcgc caaaccagcc cacggcggcg gtcatcacat ccagttgata
    19381 atcaatctgc ccgtcggcgc taaatttctt atcctgaacg atcaccggaa catcatcgat
    19441 accccactgt tttggcagca ttaatttcag gatctcgtca tcttcaatca ccaccagacc
    19501 agccagcccc atcgccacct gtcgcccggt cttgccatgt tgatgtggat ggaaccagca
    19561 ggtagcggca ggttgatcaa cgttcaacgt caccgagcgc ttgccacctg gcggaataat
    19621 tccctgtggg ccgccgtcga cttcacccgg tacttccagc ccgtgccagt gcaacgtcgt
    19681 ctcttccgtc agttggttat agatatcaac cgttaccgct ttgccgcgct gtaatttcac
    19741 cgccggcccc agcagattgc cgttatagcc ccaggtagtt gcagttttct cgccaaaggt
    19801 agactgacct gcgccaatag ttaactgaat gcgattacgg gcatcggtcg tgagcaaatc
    19861 agggatcggt aacgttgggc gttccgccgc aaagactgca cggctccaca gtggcaaggc
    19921 tgaagccaca cccagtgcga cggaatattt caagaaatca cgacgttgca tagttatttc
    19981 cttattctta agcaggcgaa acaaaatcaa acgttgagca tagtccttcc ccttacggga
    20041 aggtcaagcc gcagacacaa tttaatcgac aataatggtc gtcgcctcgc tcgcagtgtg
    20101 ctaacgttta tcttctttaa gcccctggta gaagcaatga agacgttttt cagaacagtg
    20161 ttattcggca gcctgatggc cgtttgcgca aacagttacg cgctcagtga gtctgaagcc
    20221 gaagatatgg ccgatttaac ggcagttttt gtctttctga agaacgattg tggttaccag
    20281 aacttaccta acgggcaaat tcgtcgcgca ctggtctttt tcgcccagca aaaccagtgg
    20341 gatctcagta attacgacac cttcgacatg aaagctctcg gtgaagacag ctaccgcgat
    20401 ctcagcggca ttggcattcc cgtcgctaaa aagtgcaagg ccctggctcg cgattcctta
    20461 agcctgcttg cctacgtcaa ataatccctg ataccttttt gtagaaatat tgaccgtgca
    20521 tccgcggtca atgttagcta ttatgttgcg cccttttttt acgggtgtta acaaaggagg
    20581 tatcaaccca tggccgaaaa aaaacagtgg catgaaacgc tacacgacca gtttgggcag
    20641 tactttgcgg tagataacgt tctgtatcat gaaaagaccg atcaccagga tctgatcatt
    20701 tttgagaacg ctgcatttgg tcgcgtaatg gcgctggatg gcgtagtaca aaccaccgag
    20761 cgcgacgagt ttatctatca tgagatgatg acccatgttc cgctactggc ccacggtcac
    20821 gcgaaacatg tgctgattat cggcggcggc gacggtgcca tgctgcgtga agtaacccga
    20881 cataaaaacg ttgagtcaat cacgatggtg gaaatcgatg cgggtgtcgt gtcgttctgc
    20941 cgtcagtatc tacccaacca taacgccggt agctacgacg atccgcgctt taagctggtg
    21001 atcgacgatg gcgtcaattt cgttaatcaa accagccaga cctttgatgt cattatctcc
    21061 gactgcaccg atcctatcgg tcccggcgaa agccttttca cttcggcatt ttatgaaggc
    21121 tgcaaacgtt gcctgaatcc tggcggtatc ttcgtcgcac aaaacggcgt ctgcttttta
    21181 cagcaggaag aagccatcga cagccatcgc aaactcagcc attacttcag cgacgttggc
    21241 ttttatcagg cggcgatccc gacctattac ggcggtatca tgacttttgc atgggcgaca
    21301 gataacgacg ccttacgcca tctctcaacc gaaattattc aggcgcgttt tctcgcctct
    21361 ggcctgaaat gccgttatta caatccggca gtccatacgg cagcttttgc cttaccccag
    21421 tatctgcaag acgcactggc ttcacagccg tcctaaggag aagataagaa attgaaaaaa
    21481 ctgaaactgc atggctttaa taatctgacc aaaagtctga gtttttgtat ttacgatatc
    21541 tgctacgcca aaactgccga agagcgcgac ggttatattg cttatatcga tgaactctat
    21601 aatgccaacc gtctgaccga aatcctgtca gaaacctgtt ccattatcgg ggctaatatt
    21661 cttaacatcg cccgccagga ttacgaacca cagggtgcca gcgtcactat tctggtgagt
    21721 gaagaaccgg ttgacccgaa actcatcgac aaaacagaac accccggccc actgccagaa
    21781 acggtcgttg cccatctcga taaaagtcat atttgcgtac atacctaccc ggaaagtcat
    21841 cctgaaggcg gtttatgtac cttccgcgcc gatattgaag tctctacctg cggcgtgatt
    21901 tctccgctga aggcgctgaa ttacctgatc caccagcttg agtccgatat cgtaaccatt
    21961 gattatcgcg tgcgcggttt tacccgcgac attaacggta tgaagcactt tatcgaccat
    22021 gagattaatt cgattcagaa ctttatgtct gacgatatga aggcgctgta tgacatggtg
    22081 gatgtaaacg tctatcagga aaatatcttc cataccaaga tgttgcttaa agagttcgac
    22141 cttaagcact acatgttcca caccaaaccg gaagatttaa ccgacagcga gcgccaggaa
    22201 attaccgctg cgctgtggaa agaaatgcgc gagatttatt acgggcgcaa tatgccagct
    22261 gtttaacggc tctggcggag ctcccaggct ccgccagatt tatttacttc tgctgcacga
    22321 aattgcggta agccgccacg acctgcagaa aatcctcaac gccgcatagc gacaggcttt
    22381 cttcgtcgta gtagttcatc ccctcttcca tttcatcgcc agcgaattcc agttgattgg
    22441 cgcgaaccat cacctcttcg ccgtccatcc atagcgtata ctcatgccct gcccgctgcc
    22501 aggaacgttc gctacctttc agtgcgtgcg ctgcttgttc cacttcatca agcaaggcca
    22561 ggttttcttt cacctcttca ttaaaccagt gcccgaccac ttcatgcccc atggacatac
    22621 gcacctttac cactccggta atatcgcgca gaaattcgta atccatagtg tgttcctctg
    22681 ctcccggcaa tgcaccgaaa tcgcattgcg ccattgatgt aattatcgca gccttaccgg
    22741 agaaaaacag cggaacggcg atggtttaga aataaaaaaa tgcccgggaa tctcattcac
    22801 cgggcattgt gtcgtttatg cgcagcgcgt gcgctgactt tttaaaccgc agtctggaaa
    22861 atcaccccat cggctttctc ggtgtactga gaaagctggt tgaagttcag ataacggtaa
    22921 gtatcaacgg ctgttttatc tacctgcgcc acgtaggtct ggtactcttc cggcgtcggc
    22981 agtttgccaa tcagcgccgc aacagcagcc agttccgcag aagccaggaa gacattcgcg
    23041 ccagtaccca gacggttcgg gaagttacgg gtagaggtgg aaaccaccgt tgcaccgtcc
    23101 gccacacgcg cctggttacc catacacagg gaacagccag ggatctcgat acgcgcacca
    23161 ctcttaccga agacgctgta gtagccttct tcggtcaact gtgcggcgtc catacgggtt
    23221 ggcggtgcca cccacaggcg ggtcggcaac tgacctttgt gcgcatccag cagtttacct
    23281 gcggcacgga agtgaccgat gttagtcatg caagaaccga taaacacttc gtcgatcttc
    23341 tcgccctgta ccgcagacag cggacgtgcg tcgtccgggt cgttcggagc acagaggatt
    23401 ggctctttaa tatccgccag atcgatgtcg atcactgccg cgtattccgc atctgcatcg
    23461 gcttccagca gctcaggatt cgccagccat ttttccatgc cctgaatacg acgttccagg
    23521 gtacgacgat cgccgtaacc ttccgcgatc atccacttca gcaggacgat gttggagttc
    23581 aggtattcga tgattggttc tttgttcagc ttgatggtac aaccggcagc agaacgctcg
    23641 gcggacgcat cggtcagctc aaaggcctgc tcaactttca gatccggcag accttcaatt
    23701 tctaggatgc ggccagagaa gatgtttttc ttgcctttct tctcaacggt cagcagacct
    23761 tgtttgatcg catacagcgg aatggcgtgt accagatcgc gcagggtgat gcccggctgc
    23821 attttgcctt tgaagcgcac cagaacggat tccggcatat ccagcggcat tacgcctgtt
    23881 gcggctgcaa atgccaccag accagaaccc gccgggaaag agataccgat cgggaaacgg
    23941 gtatgggagt caccaccggt accgacggta tccggcagca gcatacggtt cagccaggag
    24001 tggattacgc cgtcacccgg acgcagcgac acaccgccac ggttcataat gaagtccggc
    24061 agcgtgtggt gcgtgttcac gtcaactggc ttcggatacg ccgcggtgtg gcagaaagac
    24121 tgcatcacca ggtcagccga gaagcccagg cacgccaggt ctttcagttc atcacgggtc
    24181 atcgggcctg tggtgtcctg agagcctaca gaagtcattt tcggttcaca gtacgcgccc
    24241 ggacgaatgc ctttcacgcc acaggcacgg cctaccattt tctgcgccag cgagaagccg
    24301 cgatcgctct cagcgacatc tttcgcctga cggaacacat cactgtgcgg cagaccaagt
    24361 gcttcacgcg ctttggtggt caggccacga ccgataatca gcggaatacg gccgccagca
    24421 cgcacttcat caatcagcac gtcggttttc agttcgaagg tcgccagcag ttcgccggtt
    24481 tcgtggttac gtacttcacc tttgtacggg taaacgtcaa ttacgtcgcc catgttcagg
    24541 ttagagacat ccacttcgat tggcagcgca cccgcatctt ccatcgtgtt aaagaagatg
    24601 ggtgcaattt taccgccgag gcacaaacca ccgccgcgtt tgttcggcac atgtggaata
    24661 tcatcgccca taaaccacag aacggagttc gtggcggatt tacgcgatga acccgtaccc
    24721 acaacgtcac cgacgtacgc cagcgggaaa cctttctgtt gcagagcttc aatctgtttg
    24781 atcgggccaa caacacctgg ctggtctggc tcaatacctt cacgggcgtt tttcagcatc
    24841 gccagcgcgt gcaatgggat atccgggcgt gaccacgcat ccggtgccgg agaaaggtca
    24901 tcggtgttgg tttcgccagt gactttgaag acggtaacgg tcagtttttc agccagcgcc
    24961 gggcgattca ggaaccattc ggcatcagcc caggactgca taacctgctt cgcatattcg
    25021 ttgcctgctt tcgctttctc ttctacgtca tagaagttat cgaacatcag tagtgtgtga
    25081 gaaagtgctt tggcagcgat cggtgccagt ttggcatcat ccagcgcatc gatcagcgga
    25141 tgaatgttgt aaccaccctg catagtaccc agcagttcga tggctttttc cggagtcagc
    25201 agtggggatt tggcttcgcc tttggcgaca gcagccagga agcctgcttt aacataggcg
    25261 gcttcatcga cgccaggagg aacacggttg gttaacagat ctaacaggaa ttcttcttcg
    25321 cccgcgggcg ggtttttcag cagctctaca agcgcggcca tttggtttgc atccaggggt
    25381 ttgggcgcaa tcccctcagc ggcacgctca gctacgtgct tacggtattc ttctagcacg
    25441 acggttctcc tcgctctcat tgtcatagtg cggcaggcga ttctcttcac gctcctgtga
    25501 gacagcagtt tgtagggtaa atgcccagta ccgcaacggg cagaatagca ggattttgac
    25561 gacgtgttaa tctgtttaca aaaaagcaac ataaaaaagt ggctgaatcg ttaaggatgg
    25621 tctagagatt gtttccctcc gtaaaaactt cgttaattcg catggtgata atcacctttc
    25681 tcaacgaatc ccaagattag tcaaaattta aactaccgcc tctttatact cggattcaca
    25741 gcacctgcgg gtggcagttc gccgaccatt gcgatttcct tgagatccga attatgaaaa
    25801 tgactttgcc gtttaaaccc catgtgctgg cgctaatttg cagtgccggg ctttgtgccg
    25861 cctctgccgg gctatatata aaaagccgca cagtggaagc gcctgtggaa acgcaatcga
    25921 cacaactggc tgtgtctgac gctgccgcag ttacgcttcc tgcaacggtt tccgcacctc
    25981 ccgtaacacc cgccgtcgtc aaatccgcat tcagcactgc acaaatagat caatgggtcg
    26041 cgcccgtcgc gctgtatccc gacgccctac tttcgcaggt gctgatggca tcaacctatc
    26101 cgacaaacgt tgctcaagca gtgcaatggt cgcacgataa tccacttaaa caaggcgatg
    26161 ctgctattca ggcggtatct gaccagccgt gggacgccag cgttaaatca ctggtggcct
    26221 ttccacaatt gatggcattg atgggcgaaa acccgcaatg ggtgcaaaac ctgggcgatg
    26281 cttttctggc ccagccgcag gacgtgatgg actcggtaca acgattgcgg caactggcac
    26341 aacaaaccgg ctcgctgaag tcatcaaccg aacagaaagt tattaccaca acgaagaaaa
    26401 ctgtaccggt aacacagaca gtcacggctc ccgtcatacc atccaatacc gtttcaactg
    26461 ccaaccctgt cattacagag cctgcaacaa ccgtcatttc cattgagccc ggcaatcctg
    26521 atgtggtcta tattcccaac tacaacccaa ccgtggttta cgggaactgg gccaatactg
    26581 cgtatccgcc ggtttatctg ccaccaccag ccggagaacc gtttgttgac agctttgtac
    26641 gcggattcgg ctatagcatg ggcgttgcta ccacgtacgc actattcagc agcatcgact
    26701 gggatgacga cgatcatgac catcatcatc atgacgatga taattatcat caccacgatg
    26761 gcggtcatcg tgacggtaat ggctggcaac ataacggcga caacatcaat atcgacgtca
    26821 acaatttcaa ccgtatcacc ggtgagcatc ttactgataa gaatatggca tggcggcaca
    26881 atccaaacta ccgtaatggt gtgccctatc atgatcagga tatggcaaag cggtttcatc
    26941 aaactgatgt caacggcgga atgagtgcca cgcagctacc tgctccaaca cgcgacagcc
    27001 agcgtcaggc ggcagcaagt cagtttcagc aacgaacaca cgccgccccc gtcattacac
    27061 gagataccca acgtcaggca gcggcacagc ggtttaatga agctgaacac tatgggagct
    27121 atgacgactt ccgcgacttc agccgtcgcc aaccactgac ccagcaacaa aaggacgccg
    27181 ctcgtcagcg ttatcagtca gcttctcctg agcagcgcca ggcagttcac gagaaaatgc
    27241 agactaaccc gcagaaccag cagcgaagag aggcagcgcg tgagcgcatt cagcccgcct
    27301 cgcctgagca gcgccaggca gtccgcgaga aaatgcagac taacccacag atccagcagc
    27361 gaagagacgc agcgcgtgag cgtattcagt cagcctcgcc tgagcagcgc caggtgttta
    27421 aggaaaaagt acagcagcgc ccactgaacc aacagcaacg tgataacgcc cgccagcgtg
    27481 ttcaatcagc atcacctgaa caacgtcagg tttttcggga gaaagctcag gagagccgcc
    27541 cacaacgtct aaacgacagt aaccatactg ccaggctgaa taacgagcaa cggtcagcag
    27601 tacgcgaacg tctctctgag cgcggagcaa ggcgactgga aaggtaaatt acaggcgtaa
    27661 aaaaagcggc gtggttagcc gcttttttaa ttgccggatg ttccggcaaa cgaaaaatta
    27721 cttcttcttc gctttcgggt tcggcaggtc agtaatgcta ccttcgaaca cttctgccgc
    27781 caggcccaca gactcgtgca gagtcgggtg cgcgtggatg gtcagtgcga tgtcttcagc
    27841 atcacaaccc atttcaattg ccaggccgat ttcacccagc agctcgccgc cgttagtacc
    27901 gacaatcgca ccaccgatca cacggtgaga ttctttgtcg aaaatcagct tggtcatacc
    27961 gtctgcgcag tcggaagcga tagcacgacc agaagcagcc cacgggaagg tggcggtttc
    28021 atagctgatg cctttctctt tcgcttcttt ctcagtcaga cctacccatg caacttctgg
    28081 ttcggtatag gcgatggacg ggataacttt cggatcgaag tagtgtttct taccggcgat
    28141 aacttcagcg gcaacgtgac cttcgtgaac acctttgtgt gccagcatcg gctgaccgac
    28201 gatatcgccg atagcaaaga tgtgcggtac gttggtacgc agctgtttgt caacgcggat
    28261 gaaaccacgg tcgtcaactt ccacgccagc tttgcctgcg tcgaggtttt taccgttcgg
    28321 cacacgacca atcgctacca gcacggcgtc gtaacgctgc ggttcagctg gtgctttttt
    28381 gccttccatc gtcacataaa taccgtcttc tttcgcttca acggcggtaa ctttggtttc
    28441 cagcatcagg ttgaatttct tgctgatacg cttggtgaag actttaacga tgtctttgtc
    28501 agctgccggg ataacctggt cgaacatttc aaccacgtca atctgtgaac ccagcgcgtg
    28561 gtaaacggtg cccatttcca gaccgatgat accgccaccc attaccagca ggcgttctgg
    28621 tacttctttc agttccagcg cgtcagtaga gtcccagata cgcggatctt catgcggaat
    28681 aaacggcagt tgaatcgggc gagaacccgc tgcaatgatc gcgttgtcga agttgatcac
    28741 ggttttaccg ttctcacctt caacttccag ggtgttagcc ccggtaaatt tacccagacc
    28801 gttgaccact ttgactttgc ggcctttcgc cataccagcc agaccaccgg tcagctgatt
    28861 gatcactttc tctttccagg tacgaatctt gtcgatatcg gttttcggtt cgccgaagac
    28921 gataccgtgt tcagccagcg ctttggcttc ttcgataact tttgctacgt gcagcagtgc
    28981 tttagaaggg atacagccga cgttcaggca aacaccgcca agggtgttgt aacgttctac
    29041 gattacggtt tccagaccta aatcagcgca acggaaggca gcggagtaac ctgcggggcc
    29101 tgccccaagt accacgacct gagttttgat ttcagtactc atcatgacct ctatatattt
    29161 atctccggcg ggtctgacgt tttttttatg acgcccatta tccaccgggt cgttctatcc
    29221 gtcggtattt tacaaaattg ttaacaattt ttaaacaaca aacggcaacc gatttgtcta
    29281 ttcgctaata acctcaatac attcatgaga ttaccagaaa aaagccggcc gttgggccgg
    29341 ctcttttact tacatcacca gacggcgaat gtcagacagc gtgttgttaa tgatggtaat
    29401 gaaacgggca ccatcagcac cgtcgatcac gcggtggtcg aaggagagag aaatcggcag
    29461 catcagacgc ggcacgaact ctttaccatt ccacaccggc tccatcgcgg acttggaaac
    29521 gccgaggata gccacttccg gcgcgttcac aatcggcgcg aagtgggtag tacccaggcc
    29581 gccgatgctg gagatggtga agcaaccgcc ctgcatttcg cccgcagtca gcttaccgtc
    29641 acgcgctttc ttagaaatag tcatcagctc gcgagacagt tcgatgatgc ctttcttgtt
    29701 gacgtctttg aataccggaa caaccagacc gttcggggta tccaccgcca caccgatgtt
    29761 gatgtatttc ttcagggtca gacgctgacc gtcttccgac agcgaactgt tgaagcgagg
    29821 catctgctca agcgctgcag caacggcttt catgatgaag acaaccgggg tgatcttcac
    29881 atccagctta cgtttcgccg cttcttcgtt ctgctgttta cggaacgctt ccagctcggt
    29941 gatatcggtt ttgtcgaagt gagtaacatg cgggatcatc acccagttac ggctcaggtt
    30001 cgcaccagaa attttctgga tacggcccag ttccacttct tcgatttcac caaacttgct
    30061 gaagtccacc ttcggccacg gcagcatgcc agggatacca ccgccagtcg ctgccggagc
    30121 tgcttctgca cgtttgatag cttctttcac gtaagcctga acgtcttcgc gcaggatacg
    30181 acctttacgg ccagtgccct tcactttcgc aaggttaacg ccaaactcgc gtgccagacg
    30241 gcggatcagc ggggtcgcgt gaacgtaagc gtcattttca gcaaattcag atttgccttc
    30301 cgctttcgca gccggtgctg ctgccggggc ttcagctttt gctgccggag ccggcgctgc
    30361 tgcttcctgt ttcgcaggag ctgccgcagg cgctgcgcct tcaacttcga agatcataat
    30421 cagcgagcca gttttcactt tatcgccaac gttgactttc agttccttca cgacgcctgc
    30481 aaacggagcc ggaacttcca tagaagcttt atcgccttct acggtaatca gtgactgttc
    30541 agcggcaact ttgtcgccca ctttcaccat cacttcggtc acttcaactt cgtcaccgcc
    30601 gatatccgga acgttaactt ctttcacgcc agccgctggt gcaggggccg ctgccggagc
    30661 cgcttcctgt ttagccgccg gagctgccgc gcctgcttca cccgccactt cgaagaccat
    30721 aatcagcgag ccggtagaca ctttgtcgcc ggtgttcact ttgatttctt tcacggtgcc
    30781 tgcaaacgga gccggaactt ccatagaagc tttatcgcct tctacggtga tcagcgactg
    30841 ctcagcttca actttgtcgc caactttcac caggatttcg gtcacttcaa cttcgtcgct
    30901 gccgatatcc ggaacgttaa cgtcttttgc cgcggcagcc tcggggactg ctgccggagc
    30961 tgcttctttc ttctcttctg cctgagcagg tgcagcgtct gctgcaccgt cggcggaatc
    31021 gaaaatcata atcagtgcgc cggtctgagt tttatcgcca acagagactt tgatctcttt
    31081 aacgataccc gcctgcggag acggaacttc catagaagct ttgtcgcctt ctacggtgat
    31141 cagcgactgt tcggcttcaa ctttgtcgcc cactttgacc aggatctcgg tgatttcaac
    31201 ttcatcagcc ccgatgtccg gtactttgat ttcgatagcc attattcttt tacctcttac
    31261 gccagacgcg ggttaacttt atctgcatcg atgttgaatt tggcgattgc gtcagcaacc
    31321 actttcttat cgatttcgcc acgtttagcc agttcgccca gcgccgcaac cacgacataa
    31381 gaagcatcaa cttcgaagtg gtgacgcagg ttctcacggc tgtcggaacg accgaagcca
    31441 tcagtaccca gtacgcggta gtcgtcagcc ggtacgtaag tacggacctg ctcagcgaac
    31501 agtttcatat agtcggtaga tgccacagcc ggagcgtcgt tcatcacctg agcgatatac
    31561 ggaacgcgcg gagtttccag cggatgcagc atgttccagc gttcacaatc ctgaccatca
    31621 cgcgccagtt cggtgaagga ggtcacgcta taaacgtcag aacctacgcc gtaatctttc
    31681 gccaggatct ctgccgcttc acggacgtga cgcaggatag aaccggagcc cagcagctga
    31741 actttacctt tgctaccttc aatggtttcg agtttgtaga tacctttacg gataccttcc
    31801 tcagcacctt ccggcattgc cggcatgtgg tagttttcgt tcagcgtggt gatgtagtag
    31861 taaacgttct cttgtttttc accgtacata cgctccagac cgtcatgcat aatgacagca
    31921 acttcgtaag cgtaagccgg gtcgtaagag atacagttcg gaatagtcag cgactgaatg
    31981 tggctgtgac catcttcgtg ctgcagacct tcaccgttca gggtggtacg accggaagta
    32041 ccgccgatca ggaagccacg cgcttgctgg tcgccagccg cccagcacag atcgccgata
    32101 cgctggaagc cgaacatcga gtaatagatg tagaacggaa tcatcggcag attgttggtg
    32161 ctgtaagagg tcgccgctgc cagccaggaa caacctgcgc ccagctcgtt gatcccttcc
    32221 tgcagaatct gacctttctc gtcttcttta tagtaagcaa cctgctcgcg gtcctgcggg
    32281 gtgtactgct gaccgttcgg gctgtaaata ccaatctgac ggaacagacc ttccataccg
    32341 aaagtacgcg cttcgtcggc gatgatcggt accagacgat ctttgatcga cttgttcttc
    32401 agcatcacgt tcagggcacg aacgaaagcg atagtggtag agatctcttt gctctgctct
    32461 tccagcagcg cgccgaagtc ttgcaggctt ggcagctcaa gcttctcggt gaagttcggc
    32521 tgacggcttg gcagataacc gtgcagtttc tgacgctgtg cgtgcagata ggtatgctct
    32581 tcagaacctt ccgggaaggt gatgtacggc agtttttcga tatctgcatc agacaccggc
    32641 acattgaaac ggtcgcggat gtgacgcaca ccgtccatgt tcattttctt aacctggtgc
    32701 gcgatgtttt taccttcagc cgcgtcgccc atgccgtaac ctttaatggt atgagcaagg
    32761 attaccgtcg ctttgccttt ggtttcctgc gctttcttga atgcagcgta gattttcttc
    32821 ggatcgtgac caccacggtt cagtgcccag atctgctcgt cagtccagtc tgcaaccagt
    32881 gctgcggttt caggatattt accgaagaag tgttcacgaa cgtacgcacc atctttcgat
    32941 ttgaaggtct ggtagtcgcc gtcaacggtt tcgttcatca gctggatcag tttaccgctg
    33001 gtatctttac gcagcagttc atcccaacgg ctaccccaca tcactttgat cacgttccag
    33061 ccagcacctt cgaagatacc ttccagttcg ttgatgatct tgccgttacc ggtgaccggg
    33121 ccgtcaagac gctgcaggtt acagttgata acgaagacca ggttatccag tttttcacgg
    33181 gtagcgatgg tgatcgcacc tttggattcc ggttcgtcca tttcaccgtc gccgaggaac
    33241 gcgtaaacgg tctgtttaga ggtatctttc aggccacggt gttccagata tttcaggaat
    33301 ttagcctggt aaatagcacc aatcggaccc agacccatag atacggtcgg gaactgccag
    33361 aattccggca tcaatttcgg gtgcggatag gaagagaggc cattgccgtg aacttcctga
    33421 cggaagttat ccagctgctc ttgagtcaga cgaccttcca ggaaagcacg agcgtacacg
    33481 cccggggaga tgtggccctg gaagtaaacc aggtcgccgc catcctgctc gttgcgtgca
    33541 cggaagaagt ggttaaagca cacatcataa atggttgcgg aagactggaa ggacgccatg
    33601 tggccgccca gttcgaggtc ttttttcgac gcacgcagaa ccgtcatgat ggcgttccag
    33661 cggatagctg aacgaatacg gcgttccagt tccagattac ccggatactc cggttgttct
    33721 tcaacgggga tggtgttgat gtagttgctg ataccggtgc ctgcggctac gttgacaccg
    33781 cctttgcggg cttcagcaag cagttggtcg atcagatact gagcacgctc aacaccttct
    33841 tcacggatga ccgattcgat cgcctggagc cagtcgcgag tttcgatcgg atccacgtca
    33901 tttgggaaac gttctgacat gggttattcc ttatctatct aataacgttg agttttctgg
    33961 aacctgtccc attgaactct cgccggaaag ctcaataaga caggttctac gtttagttgc
    34021 cgcgctttta tatgcgcttg atttacaaca tcttctggat aatttttacc agaaaaatca
    34081 ctaattcttt cgttgctcca gacgacgcag agaacgctca cggcggcttt cttcacggct
    34141 tctgtcgagc aaaatttctt cgataaaggc cagatggcga tgcgatgctt cgcgcgcttc
    34201 ttccggctta ccggccataa tcgcttcaaa tatgcgggtg cggtgactac tcaccagcgg
    34261 cagcatctcg cgacgcgaat agagcaattc gaagttctgg cggacattct gggccaacat
    34321 cggctccata caccttagca gatgaagcag aaccacattg tgggccgctt cggtgacggc
    34381 aatctgatac tggagtacgg cgtttgattc cgcgtccaga tcgccagact gctgcgccag
    34441 ttctatggcg tggtggagtt cacggatgcg ttccttgtct tcatcggtac tacgcagcgc
    34501 ggcgtaataa gcggcgatac cttccagggc gtgtcgtgtt tcgagcaagt catactgtga
    34561 ttcaggatgg tcggagagca gctccaccag cggatcgctg aagctttgcc ataggctgct
    34621 ctggacaaaa gtgccgccac cctggcgacg aagcaacaag cccttcgctt cgagacgttg
    34681 aatcgcctca cgcaatgagg gacgggagac gtcaaactgt tttgccagtt cgcgttccgg
    34741 tgggagtttt tcgcccgggc ggagagtgcc ttcgaggatc aaaaactcca gttgctgctc
    34801 aatcacatcg gagagttttg gttggcggat tttgctgtag gccatgagtt cctgtcttaa
    34861 gccacttgcc gaagtcaatt ggtcttacca atttcatgtc tgtgacgcta aagtaacaaa
    34921 gtattcacct tatgtccata caggttttga ttgaaatcat gaaactgtgc acattttaac
    34981 aacttgacat atataacgtt tcaaagttgt aactatgcac aaatgtagac tttacgtagg
    35041 aaagggattt tttaacgat
//
LOCUS       DBFIFM010000023        35001 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.023, whole genome shotgun sequence.
ACCESSION   DBFIFM010000023 DBFIFM010000000
VERSION     DBFIFM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.023"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(86..817)
                     /gene="rlmB"
                     /locus_tag="N7C74_001036"
     CDS             complement(86..817)
                     /gene="rlmB"
                     /locus_tag="N7C74_001036"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HGU2430956.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(998..3439)
                     /gene="rnr"
                     /locus_tag="N7C74_001037"
     CDS             complement(998..3439)
                     /gene="rnr"
                     /locus_tag="N7C74_001037"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HGU2430957.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLMLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVSDWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3478..3903)
                     /gene="nsrR"
                     /locus_tag="N7C74_001038"
     CDS             complement(3478..3903)
                     /gene="nsrR"
                     /locus_tag="N7C74_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HGU2430958.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGLMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4108..5406)
                     /gene="purA"
                     /locus_tag="N7C74_001039"
     CDS             complement(4108..5406)
                     /gene="purA"
                     /locus_tag="N7C74_001039"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HGU2430959.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5510..5707)
                     /gene="yjeT"
                     /locus_tag="N7C74_001040"
     CDS             complement(5510..5707)
                     /gene="yjeT"
                     /locus_tag="N7C74_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HGU2430960.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5789..6793)
                     /gene="hflC"
                     /locus_tag="N7C74_001041"
     CDS             complement(5789..6793)
                     /gene="hflC"
                     /locus_tag="N7C74_001041"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HGU2430961.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6796..8055)
                     /gene="hflK"
                     /locus_tag="N7C74_001042"
     CDS             complement(6796..8055)
                     /gene="hflK"
                     /locus_tag="N7C74_001042"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HGU2430962.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8141..9421)
                     /gene="hflX"
                     /locus_tag="N7C74_001043"
     CDS             complement(8141..9421)
                     /gene="hflX"
                     /locus_tag="N7C74_001043"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HGU2430963.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9498..9806)
                     /gene="hfq"
                     /locus_tag="N7C74_001044"
     CDS             complement(9498..9806)
                     /gene="hfq"
                     /locus_tag="N7C74_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HGU2430964.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9892..10842)
                     /gene="miaA"
                     /locus_tag="N7C74_001045"
     CDS             complement(9892..10842)
                     /gene="miaA"
                     /locus_tag="N7C74_001045"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HGU2430965.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLGGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10835..12682)
                     /gene="mutL"
                     /locus_tag="N7C74_001046"
     CDS             complement(10835..12682)
                     /gene="mutL"
                     /locus_tag="N7C74_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HGU2430966.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRPIPENRVAAGRNHFAEPAVREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12692..14029)
                     /gene="amiB"
                     /locus_tag="N7C74_001047"
     CDS             complement(12692..14029)
                     /gene="amiB"
                     /locus_tag="N7C74_001047"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HGU2430967.1"
                     /translation="MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVVKRVETPTVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANNGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14048..14509)
                     /gene="tsaE"
                     /locus_tag="N7C74_001048"
     CDS             complement(14048..14509)
                     /gene="tsaE"
                     /locus_tag="N7C74_001048"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HGU2430968.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14481..16028)
                     /gene="nnr"
                     /locus_tag="N7C74_001049"
     CDS             complement(14481..16028)
                     /gene="nnr"
                     /locus_tag="N7C74_001049"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HGU2430969.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVALIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16027..17166
                     /gene="queG"
                     /locus_tag="N7C74_001050"
     CDS             16027..17166
                     /gene="queG"
                     /locus_tag="N7C74_001050"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HGU2430970.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKRLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAMAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17149..17202)
                     /locus_tag="N7C74_001051"
     CDS             complement(17149..17202)
                     /locus_tag="N7C74_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2430971.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17436..17511)
                     /locus_tag="N7C74_001052"
     tRNA            complement(17436..17511)
                     /locus_tag="N7C74_001052"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17476..17478),aa:Gly,seq:gcc)
     gene            complement(17547..17622)
                     /locus_tag="N7C74_001053"
     tRNA            complement(17547..17622)
                     /locus_tag="N7C74_001053"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17587..17589),aa:Gly,seq:gcc)
     gene            complement(17659..17734)
                     /locus_tag="N7C74_001054"
     tRNA            complement(17659..17734)
                     /locus_tag="N7C74_001054"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17699..17701),aa:Gly,seq:gcc)
     gene            complement(17945..18490)
                     /gene="orn"
                     /locus_tag="N7C74_001055"
     CDS             complement(17945..18490)
                     /gene="orn"
                     /locus_tag="N7C74_001055"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HGU2430972.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDGWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18585..19637
                     /gene="rsgA"
                     /locus_tag="N7C74_001056"
     CDS             18585..19637
                     /gene="rsgA"
                     /locus_tag="N7C74_001056"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HGU2430973.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19734..20702
                     /gene="asd"
                     /locus_tag="N7C74_001057"
     CDS             19734..20702
                     /gene="asd"
                     /locus_tag="N7C74_001057"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HGU2430974.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20724..24047
                     /gene="mscM"
                     /locus_tag="N7C74_001058"
     CDS             20724..24047
                     /gene="mscM"
                     /locus_tag="N7C74_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HGU2430975.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24198..25700)
                     /gene="yjeM"
                     /locus_tag="N7C74_001059"
     CDS             complement(24198..25700)
                     /gene="yjeM"
                     /locus_tag="N7C74_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HGU2430976.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(25919..26896)
                     /gene="epmA"
                     /locus_tag="N7C74_001060"
     CDS             complement(25919..26896)
                     /gene="epmA"
                     /locus_tag="N7C74_001060"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HGU2430977.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            27221..29029
                     /gene="frdA"
                     /locus_tag="N7C74_001061"
     CDS             27221..29029
                     /gene="frdA"
                     /locus_tag="N7C74_001061"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HGU2430978.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTKRDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            29022..29756
                     /gene="frdB"
                     /locus_tag="N7C74_001062"
     CDS             29022..29756
                     /gene="frdB"
                     /locus_tag="N7C74_001062"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HGU2430979.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            29767..30162
                     /gene="frdC"
                     /locus_tag="N7C74_001063"
     CDS             29767..30162
                     /gene="frdC"
                     /locus_tag="N7C74_001063"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HGU2430980.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            30173..30532
                     /gene="frdD"
                     /locus_tag="N7C74_001064"
     CDS             30173..30532
                     /gene="frdD"
                     /locus_tag="N7C74_001064"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HGU2430981.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            30595..31728
                     /gene="ampC"
                     /locus_tag="N7C74_001065"
     CDS             30595..31728
                     /gene="ampC"
                     /locus_tag="N7C74_001065"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HGU2430982.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            31816..32349
                     /gene="blc"
                     /locus_tag="N7C74_001066"
     CDS             31816..32349
                     /gene="blc"
                     /locus_tag="N7C74_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HGU2430983.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(32346..32663)
                     /gene="sugE"
                     /locus_tag="N7C74_001067"
     CDS             complement(32346..32663)
                     /gene="sugE"
                     /locus_tag="N7C74_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HGU2430984.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(32839..32985)
                     /gene="ecnB"
                     /locus_tag="N7C74_001068"
     CDS             complement(32839..32985)
                     /gene="ecnB"
                     /locus_tag="N7C74_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HGU2430985.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(33096..33221)
                     /gene="ecnA"
                     /locus_tag="N7C74_001069"
     CDS             complement(33096..33221)
                     /gene="ecnA"
                     /locus_tag="N7C74_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HGU2430986.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(33273..33839)
                     /gene="efp"
                     /locus_tag="N7C74_001070"
     CDS             complement(33273..33839)
                     /gene="efp"
                     /locus_tag="N7C74_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGU2430987.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            33881..34909
                     /gene="epmB"
                     /locus_tag="N7C74_001071"
     CDS             33881..34909
                     /gene="epmB"
                     /locus_tag="N7C74_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HGU2430988.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRAGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         8301 a         9604 c         8923 g         8173 t
ORIGIN      
        1 aatatttgca atacgtaatt atcttaccag ctatagacaa aaaaaaacca tccaaatctg
       61 gatggctttt cataattcag aggaactagc tgcgctgacg aaccgcttca aataagcaaa
      121 ttccggttgc aaccgaaacg ttcagggaag aaacacttcc tgccattggg atgctgatca
      181 actcatcgca atgttcacgg gtcaggcgac gcataccttc accttccgcg cccatcacca
      241 gcgccaggcg tccggtcatt ttgctttgat agagcgtatg atccgcctca cctgccgtac
      301 cgacgatcca gatattctct tcctgcaaca tacgcatggt gcgcgcaagg ttagtcaccc
      361 gaatcagtgg aacgctttct gccgcgccgc aggctacttt tttcgccgtg gcgttgagct
      421 gtgcggagcg atctttcggc acaatcaccg cgtgaacgcc agcagcgtcc gcgctacgca
      481 ggcacgcgcc gaggttgtgc ggatcggtta caccgtcgag gatcagcagg aacggttgat
      541 cgagcgaagc gatcagatcc ggcagatcgt tttcctggta ctgacgtcct ggcttcacgc
      601 gggcgataat gccctgatgc acggcaccgt cgcttttctc gtcgagatat tggcggtttg
      661 ccaactggat aaccacgccc tgggactcaa gggcgtggat cagcggtaac agacgtttat
      721 cttcacggcc ttttaaaata aagacttcct gaaaacgttc aggggcgcgc tccagcaggg
      781 cctgcactgc atggatgccg taaatcattt cgctcattaa tgtactcgtt gtttacgttt
      841 tgcctgatgc actacgttta tccggtctac atgatccctg caatatattg aatttgcgtg
      901 attatgtagg ccggataagg cgttcacgcc gcatccggca tgaacaaagc gcattttgtc
      961 agcaatctaa ccctcttctg tttgaagagg gtaatgatca ctctgccact tttttcttcg
     1021 ccgcacgctt cgctttggtc gctgcagcta ttttctgcgt tttcgccgat ggctttttcg
     1081 cttttctcgc gtctttcttc gctgctttcg gcttcgtttt tttctcaccg cggaaggcgc
     1141 tgtctggctc aaagtttacc tttttaccga cctgacgacg cttgccgcct tttttacctg
     1201 catcgccttt tttcgctttc tcgcgcgccg ttttaccgac gttgcgcggt gcgcgttcgc
     1261 tggagatcag gctaaagtcg attttgcgct cgtccatatt aaccgcttcg acgcgaactt
     1321 ccacgcgatc gcccaggcga taagtctggc cgctggattc acccatcagg cgttgcccta
     1381 cctggtcaaa gcgatagtag tcattgtcca gcgaagagac atggaccagc ccatcaatga
     1441 acaagtcgtc cagacggacg aagaagccaa agccagtgac gctggaaatt acgcctttaa
     1501 agacgttacc tacctggtcg agcatgaagt cacacttcag ccagtcagac acatcgcgcg
     1561 ttgcttcgtc ggcacgacgt tccgccatcg aacagtgctg acccaattgc agcatctctt
     1621 ccatcgaata atggtagcca ccggtttcag tggtgttgcc ctgatgcccc tgctctttcg
     1681 ccagcagata cttaatggcg cggtgcagca tcaggtcagg ataacgacga atcggcgaag
     1741 taaagtgcgc ataggactgc aatgccaggc caaagtgacc acggttttcc ggatcgtaaa
     1801 tcgcctgttt catcgagcgc agcagcatgg tttgcagcat ttctgcatca ggacggtcgg
     1861 caaccgactc cagcagctcc gcgtagtcgc gcggttccgg cttgttaccg cccggcagtt
     1921 ccagccccag ctctgccagc actgaacgga aagaggtaat cgcttcggtg ctcggcttgt
     1981 cgtgaatacg gaacagtgcc ggttctttcg ctttctcaac gaaacgcgcc gccgagatat
     2041 tcgccagaat catgcactct tcaattaact tgtgtgcgtc gttacgctga gtctgttcga
     2101 tacgttcaat acggcgttca gcgttgaaaa tgaacttcgc ttcttcgctc tcaaatgaga
     2161 tcccgccgcg ttcttcacgg gctttatcca gcactttata gaggttatgc aactcttcga
     2221 gatgcttaac cagcggggcg tactgctcgc gcagatcctg atcgccctgc agaatatgcc
     2281 agactttggt gtaggtcaga cgcgcgtgag agctcatcac cgcttcgtag aatttgtagc
     2341 cagtcaggcg gcctttcgac gaaaccgtca tctcgcacac catacacagg cggtctacct
     2401 gcggattgag cgagcacaga ccgttagaga gcacttccgg cagcatcggg ataacctgcg
     2461 aagggaagta caccgacgta ccacggttac gcgcttctct gtccagcggc gttggcggac
     2521 gcacatagta gctgacgtca gcaatcgcga cccataaacg ccagccgccg ccgcgttttt
     2581 tctcgcagta aaccgcatcg tcaaagtcac gggcgtcttc gccatcaatg gtgaccagcg
     2641 gtaaatcgcg caaatcgaca cggcccgctt ttgcttcttc cggcacttct tctttcagcc
     2701 cagcaacctg ttgctcaaca gcctgcggcc agatgtacgg aatttcatgg gtacgcagag
     2761 cgatatcaac cgccatgccc gtgcccatat tgtcgcccag cacttcgacg attttaccca
     2821 ccgctttggt acggcgagtc gggcgttggg tcagttcaac aaccaccaca aagcccatcc
     2881 gcgcgcccat gatctgatcg ggcgggatta agatatcgaa gctcagacgg ctatcgtcag
     2941 gaaccacaaa gccgacgccc gcttcggtaa agtagcgacc aacaatctgg ctggtttttg
     3001 gcaccagtac gcggacaata cgcgcttcac gacgaccttt acggtcagca cccagcggct
     3061 gagccagcac ctgatcgcca tgaatgcagg ttttcatctg ctcgctggag agatacaaat
     3121 catctttacg cccttcaacc cgcagaaagc cgtagccatc acggtggcca ataacggtac
     3181 ctttcaccag gtcgaggcgt tccggcagcg catagcactg acgacgagta aagaccagtt
     3241 gaccatcgcg ctccatcgcg cgcaggcggc gacgcaggcc ttcaagctgc tcttcgcctt
     3301 caatgtgcag ttctaccgcc agctcatcac ggctggccgg tttttcacgt ttggttaaat
     3361 gttcgaggat aaattcccga ctagggatgg gattcgcgta tttttcagct tcgcgttcct
     3421 ggaaaggatc ttgtgacatc tcggttcctc cgttgtcatc tctgatgaag attttcgtca
     3481 ctccaccagc aataatttat aaagcggttg attctcttca accaaatcgg caagcgtgta
     3541 gttatccagt tccgtaagaa aactttgcac ggccttagaa agcgcctgtt tcaaccgaca
     3601 ggcaggtgta atgtggcaaa actcactgct gcaattcacc agcgataagg gctccagctc
     3661 gcgcaccaca tcaccaatac gtatcgcact cgccggttta cccaggcgaa tgccgccatt
     3721 ttttccacga acagcagtca cgtagccggc acgactaagt tgattgatta ttttgaccat
     3781 atgattacgg gagacgccgt agacgtcagt cacttcagaa atactggtca tcagcccttc
     3841 tggcaatgac gccatgtaga tcagcgcacg taatccgtaa tcagtgaaac tcgttaactg
     3901 cacatcaacc tcaaaaggga aatcgggaaa aaatacattt atattgatga taaaccagcc
     3961 acaagctgtg tcgctaattt atttcagttc ggggaaggaa aaaagcgagg atttaacact
     4021 gtgtcggata gcgtaatggc ttaaccgaca cagcaatgta tcacactgtt agcccggcag
     4081 gcaaaatatc tgccaggcgt accagaatta cgcgtcgaac gggtcgcgca gaatcatggt
     4141 ttcagtacga tccggaccgg tagagatgat atcgatcggt acaccggtca gctcttcaat
     4201 gcgcttgatg tagttcagcg ccgcctgcgg caggccgcta cgatctttca cgccgaaggt
     4261 ggattcagac cagcccggca tggtttcgta aatcggctct acacctttcc agtcgtcagc
     4321 tgccagcgga gtggtagtca cttcacgacc atccggcata cggtaagcca cacagagttt
     4381 cacctctttc aggccatcca gaacgtccag tttagtcagg cagaagccag acagggagtt
     4441 cagctgtacc gcacgacgaa cggcaacggt gtccagccag ccggtacgac gacgacgacc
     4501 cgtagttgcg ccgaattcgt taccctgctt gcagaggaac tcgccagttt catcaaacag
     4561 ttcagtcggg aacggacctg cacccacacg agtggagtaa gctttgagga tacccagaac
     4621 gtaatcaaca taacgcgggc ccaggccgga accggtcgcc acgccaccag cagtggtgtt
     4681 ggaagaagtt acgtacggat aagtaccgtg gtcgatatcc agcagcgtac cctgcgcacc
     4741 ttcgaacatg acgaaatcgc cacgctgacg cgcctggtcg agcaggtcag aaacgtcaac
     4801 caccatagaa gtcaggatgt cggcaacagc catcgtatca tccagaactt tctggtaatc
     4861 aaccgcttca gctttgtagt agttaaccaa ctggaagttg tgatattcca tcacttcttt
     4921 cagtttttca gcgaaggttt ctttgtcgaa aaggtcgcca acacgcagac cgcgacgtgc
     4981 cactttatct tcataagcag gcccgatacc acgaccggtg gtgccgatcg ctttcgcgcc
     5041 acgcgctttc tcacgcgcgt tatccagcgc aacatgataa tcaaggatca gcggacatgc
     5101 ttcagacagc agcagacgct cacgaacggg gatgccacgg tcttccagtt ctttcatctc
     5161 tttcatcagc gcggccggag acagcacaac accgttaccg atgatgctag ttacattctc
     5221 gcggagaata cctgatggaa taagatggag aacggttttt tcaccgttga ttacgagagt
     5281 atggcctgcg ttgtgaccgc cctggtagcg tacaacatat ttagcccgtt cagtcagaag
     5341 atcgacgatc ttacctttac cttcgtcacc ccattgggtg cccagtacga cgacgttgtt
     5401 acccattttt caaaatcacc gtttgcttaa aaatggattc taccatcgct ttttcagagt
     5461 tacagcactt tttgcactca aaaatgacct cgatcagtct gttttttgtt cagccaatcg
     5521 ttttcctcaa catgtagtag accacaacgc ccgcaaccac aagtccaccg ccaaaacgac
     5581 gtaaaatatt atcgggcaaa ttggtcatcg cagagatcat cttcttccat gccttcgggt
     5641 aaagcatcgg ccctaaacct tccagtacca aaaccagggc aagcgccagc cagattgtcg
     5701 aattcattca ttatccttat agaaaaagaa aaccaccgac atccctgagg atgcggtggc
     5761 tttattgacc tgtaccgcag tcgttatatt aacgcgttgc ggaagtcggc gtcttcatgt
     5821 agcggaagaa atcgctatcc gggctcatga ccatcacgtc ctgattgcca gagaagctgt
     5881 tctcataagc acgcaggcta cggatgaatg cgtagaagtc cggatcttta ctgaatgcat
     5941 cagcaaacag tttggctgct tcggcatcgc cttcaccgcg catgatgcgg ccctgacgct
     6001 cagcttctgc cagcgttctg gtcacttcat agtcggcagt cgcgcgcagt ttttccgctt
     6061 cttcctgacc ttgtgaacgg tgacgacgcg ctaccgcttc acgctcggcg cgcatacggt
     6121 tgtagatcgc ttcagacact tcggtcggca ggttgatctg cttaattcgc acatcgacaa
     6181 cttcaatacc cagcgccgcc atactgttcg ggttgatgac cggaactttg cccttcgtct
     6241 cagccgttac gcgctctgcc gcttcggcaa tggcgttatc tgccgccggg gtagtaactt
     6301 catcttctgt acccgcagaa ccggagttca gcgcgtcacg tacttcgagg gtcagacgac
     6361 cacgggaatc ggtgacgatg tctttcacgt ccaggcgacc aatttcagaa cgcagacggt
     6421 cagagaactt acgtttcaac agcacttccg cttgcgaaat gtcgcccccc cccgttgcca
     6481 ggtagtaacg gctgaaatcg ctgatgcgcc atttgatgta agagtcgacg atcaggtctt
     6541 tcttctcttt ggtcacaaag cggtcggcct ggttgtccat ggtctgaata cgtgcgtcga
     6601 gcattttcac cgtttcaatg aacggtatct tgaaatgcag acccggctca taaaccagag
     6661 gtttgttgtc atcgtcacgc agtaccttac caaaacgcag cgtaataccg cgctcacctt
     6721 ctttgacgac aaagacagac atgtaaagca ctaccagcac gatgatgata atcgcgataa
     6781 ctgacttacg catcgttatt ccccctgacg ctggtagtcg ttacgctgcg cattggcgcg
     6841 gcgttggtcc ataatatcgc cctgactggt ggacgacgtg ttgcttgctc cactggttgt
     6901 ggaggaagag gctggcggca gacgcagcag attgctggca ccgttatcgc tcttcgccgc
     6961 aggggcgtta ccacctttca gcatctggtc taacggcaga accatcaggt tgccaccttt
     7021 atcgttaacc agcactttgc gggtgttacc caacactttt tccatcgtct cgatatacag
     7081 acgctcgcga gtaatttccg gcgcggcttt atattccggc agaagtttag caaaacgcgc
     7141 cacttcaccc tgagcttcca ggatggtctg ggccttgtac gcacgcgcct cttcgaggat
     7201 acgttgcgcc tgaccgttcg cacgcggctg aacttcgttg gtatacgctt ctgcttcacg
     7261 aatgtattgc tgttcgtttt cacgcgcggc aatcgcatcg tcaaacgccg cttttacttc
     7321 ttccggcgga cgagcagcct ggaagttgac gtccagcagc gtgataccca tgtcatacgg
     7381 acgaatcgtc tcttccagtt cgcgctgagt atcgctacga atcacggtac gaccttccgt
     7441 cagaatgcgg tccatggtgt atttaccgat aactccacgc agggcgctgt cggtagcctg
     7501 acgcaggctg tcatccgggc tggtcacgct atacagatat ttttccggat tggtgacgcg
     7561 gtactgcacg ttcatctcaa cgcgcactac gttctcgtcc gacgtcagca tcacaccaga
     7621 agctgccagt tcacgcacgg cttccacgtt caccggtttg acttcgtcga taaacgtcgg
     7681 tttccagttc agacctggct caaccagatg gctgaattta ccaaagcgtg ttaccacgcc
     7741 gcgttcggct tctttaatgg tatagaaacc actggccgcc cagataatga caatcgctgc
     7801 cgctgcgatg gtaacgacac gaccgccaag ctgcgggcgc gggccttgcg atgaactgcc
     7861 accgccagat ccggtgcctt taccgccgcc cagaccaccg agctttttgc tcagtttgcg
     7921 gaagatatca tctaaatcag gtggcccttg atcgcgaccg cctttgtttc catttccctc
     7981 agagttgccg ccaggtttgc tgcttcccca cgggtcgcgg tcttgtccgt tattaccggg
     8041 ctgattccac gccatgtttg tgctccatat ttgttatgcg gtgatccctg ttgtctttcg
     8101 acgccagagg atatgactcc acgcttcaga cgctacgccg ttagatcagg taatcgatca
     8161 acgccggttc ttgtttacag aggcgacgcc agtcaacgat cggcatacga acttgcagac
     8221 ttacgctgcc gtcctcctcc atccactctt tttctattgc ctgaagctga taaaaacgac
     8281 ttctcagacg cccttcctgc ggtggcagac gcaatgtatg ctgcgccacc tcgccggaaa
     8341 gccgctccgt caaagcctga aaaagctgtg gtatccccgc tccggtctgt gcggaaagcc
     8401 agacacggat cggtttgttc tcttcgtccc gatcaatacg cggttcgaaa tcttccagca
     8461 tatcgatctt gttcatcacc agcagggttg ggatctcgtg agcgtcgatc tcttcaagaa
     8521 ccgtattcac cgcttcgatg ttttcttgta cacgcacatc cgccgcatca atcacatgca
     8581 gcagcaatgt ggcttgccgc gtctcttgta acgtggcttt aaatgctgcc accagatcgt
     8641 gcggcaggtg gcgaataaac cctacggtat ctgcaagtac ggtttcaccg acatctgcaa
     8701 cgtcaatacg ccgcaacgtc gggtcgaggg tggcaaacaa ctggtctgcc gcgtagaccc
     8761 gcgcttcggt gatgcgattg aaaagggtag atttaccggc gttggtatat cccaccagcg
     8821 aaacagtagg aacgtcggct ttgatacgcg attgccgccc ctgctcacgc tgcttttcaa
     8881 ctctttccag gcgcgactgt atctgcacga tgcgattacg caacaaacga cggtcggttt
     8941 cgagctgggt ttcacccgga ccacgcaaac caatcccgcc tttctgtctt tcaaggtggg
     9001 tccagccacg caccaggcgt gtagccagat ggcgcagctg cgccagctca acctgcaact
     9061 taccctcatg ggtacgcgca cgttgggcga aaatatctaa aataaggccg gtgcggtcga
     9121 taacacgaca ctcgcacaaa cgctccaggt tacgctcttg cgccgggctc agggcatggt
     9181 caaaaagaac gaccgaagca cccgtcgctt tgacagcttc cgcaatttca actgctttac
     9241 cttcacctac aaaatacttt gggtgcggcg ctttacggct accggtaatc acctgcaatg
     9301 cttcgacacc ggcggaagag accagagatt caaactcctg gaggtcttcc atatctttgt
     9361 cttgcgtaaa atagatgtgt accagtaccg cctgctcacc agcatcataa cggtcaaaca
     9421 agcgtataac cctctaaata gatcagcggg gaacgcagga tcgctggctc cccgtgtaaa
     9481 aaaaacagcc cgaaacctta ttcggtttct tcgctgtcct gttgcgcgga agtattctgc
     9541 gcgctgctac catgatggta gttactgctg gtaccgccac cggcgttgtt actgtgatga
     9601 gaaaccgggc gagacgggac aacagtagaa atcgcgtgct tgtaaaccat ctggctgacc
     9661 gtgtttttca acaggatcac gaactgatca aaagactcga tttgcccttg cagcttaata
     9721 ccattcacca aataaataga aactggaaca cgttcccgac gcagtgcgtt caggaacgga
     9781 tcttgtaaag attgcccctt agccattctc tcttttcctt atatgcttat ttgtactttg
     9841 aacctttcga ttctgaaaaa attgcgcacg atacgtctca attgtacaca ttcagcctgc
     9901 gatagcacca acaacctgta atacttcgtc acgcgcctgt tctggttttt cactgtcaag
     9961 ccagtgaacc ccttcccaac cacgcagcca ggttatctgc cgcttcgcca actgtctcgt
    10021 ggcgcaaaca cctcgataaa ccatttcatc gtatgagatt tcgcctccaa ggtaagacca
    10081 catctggcga taacccacgc aacgaatgga aggcaagtcc gtatgcaaat ctcctcgggc
    10141 aaaaagcgcc cggacttctg cttcaaaacc tgaagccaac atctgatgaa aacgctgctc
    10201 aattcgttga tggagcagtt cacggctcgc cggggcgatg gcgaactgat gcacctgata
    10261 cggtagagcg tctcctgacg tttgcgtcag ttccgttaaa gttttacccg aaatgaaaaa
    10321 aacttccagt gcccgggaaa gcctttgtgg atcatttgga tgaatccttg ctgccgcaac
    10381 cggatcgatc tcctgaagtt gacgatgcaa tgactcccaa ccttgctctg ccgcctgttg
    10441 ctcaattctg gcccgtactt ccgggtctgc cgacggtagc ggcgacaacc cttccagcaa
    10501 tgccttgaaa tacaacatcg taccgcccac taacagtggg atccgccccg ccgcggtgat
    10561 atcggccatt tccgccagcg catcgcggcg aaaatcagca gccgagtaag cctgcgacgg
    10621 atcgcgaata tccagcaatc ggtgcggcgc ggcgagtaac tcttcagcgt tcggcttcgc
    10681 cgtcccgata tccatccctt tgtaaataag ggcagaatca acgcttatca actctactgg
    10741 taaaatttta cgcagctcaa tggctaacgc cgttttaccg gaggccgtcg gccccatcaa
    10801 aaaaatcgcc ttaggcaggc tcgccttact gatatcactc atctttcagg gcttttatcg
    10861 ccggatgtaa atcaacagat tgtaacagac cacccggcgg cgttttcaca agttgcggac
    10921 ataaccgttc cacgtccgcc agcagggtta tggcctgtgc cattgaccac tgcgcatgtt
    10981 cgctcatcag atttcgtgca atccactgcg caatattgcc aggttcgaat acggactgct
    11041 tcgctaggta gcctatcagt tcaggaatca agatttgtaa attttgttgg cgtaagggta
    11101 aaggcacggc cctgatggtc acatgctgtg catctgactg gaaatcaata cccaattccg
    11161 ccagggcaga ctgcgctttt tctaatgccg atttttcttc gccagaaact tttagccgca
    11221 acggaatcag caacggctgg gcgcaaacgg gcgcttcacc cggcgtcagt tgtacctgac
    11281 gcagccaacg ttctgccact ggcaaggcta aaagtgaaat gttgccgtcg cgctccagca
    11341 acgcacagtc ggaatggacg atagtcagta cccgaccaaa actctgactg ttcgccgcaa
    11401 gtgcaggttc ctgcggttcc ggcgcttttg gtttttgcat cggcgcgggc gtttgcaaaa
    11461 gctggcgata cacttcacct tgctgtttct ggtaacctgg ctgcgcattc ggccaggggg
    11521 cagccggacg actacctgat gctggcgcag gagtgtagcg cggagctact ggctcacgaa
    11581 ctgccggttc agcaaagtga ttgcgccccg ccgccacgcg gttttccgga atgggacgcg
    11641 gtgcaggttg gggttcatcg tccagcggta gcggcgtttc cagttgctgt tgtagcacgc
    11701 tcagcacgcc ctgatagata aagtcatgca ccagacgcga ctgatgaaaa cgcacttcgt
    11761 gtttggcggg gtgcacgttg acgtccacct gatgcgggtc gatctccaga tacaacacaa
    11821 atgcaggttg ctgatcggcc cccagtttgt cttcacaggc ctggcggatc gcgtgattga
    11881 tcaggcgatc gcgcatcatg cgaccattca cgtagcaata ctgaatttcc gccagtgcgg
    11941 gcgtggtgtg atttggatcg gccacccagc cgcgcagcgt gagatcgccg tgttgccatt
    12001 caatcgccag cgcttgttcg agaaaagcgg tgccacaaat cgcgcctaag cgccgttctt
    12061 tttgcccgcc ttccggcacc gcgcggtact ggcgtacgat tttgccgtta tgcgacaggt
    12121 tgatcgtgac gtcgaaacgc gccagtgcga tgcggcggat gatttcatcg atatggttaa
    12181 attcggtttt ctcggtgcgc aggaatttgc gccgcgccgg ggtgttgtag aacaggtcca
    12241 gtacctccag cgtcgttccc accggatgcg ccgccggttt aaccgtcacg tccatatcgc
    12301 gcccttcggc ataggcctgc caggcttcct gctgttctgc ggtgcgtgaa gtgagcgtca
    12361 ggcgggaaac cgaactgata ctcgccagcg cctcaccgcg aaagcccagg ctgataatgg
    12421 cttcgagatc gtccagagag gcgattttac tggtggcatg acgcgccagc gccagcgcca
    12481 gctcgtcttt tttgatacca cagccgttat cacgaatgcg gataagtttc gccccaccgc
    12541 gttcgatatc aatatcgata cgcgtcgcac ctgcatcgag gctgttttcc accagttctt
    12601 tgactaccga cgcaggtcgc tcgaccacct cacctgcggc aatctggttc gccagttgtg
    12661 gcggtaagac ctgaattggc atcaatcgtc cttagtttgg cagcgtgcga tctggcgtcg
    12721 tcaccgtact ggcagtttgt gccgttgcac cctgcggcgc agattgcatc ggatgcgcaa
    12781 ggaaataatt gcgcaggcct ttgtaaatgg cttctgccag ctgttgttgg taatcgtcgc
    12841 tcgccagcaa acgttcttcg ctgttgttgc tgataaaacc ggtttcgacc agtactgatg
    12901 ggatatccgg cgagcgcaga acgccaaggc tggcgtgttc tggtcgacgt ttatgtattt
    12961 cgccaatgcg ttgcaactga ctgatcatac tggtcgctac atcataccct acccgctggg
    13021 aatgaccgaa ctgtaaatcc agcaccgcct ggcttaaata ggggtcagac tgactgttcg
    13081 ccagcacatc acccgccccg cccagcagct ccgactgttt ctcgtgctgc tccagccagc
    13141 tggccatttc gctgttggcg cgacggttag agagcaccca tacggaagcg ccagtcgcac
    13201 tgcggttcgg tgcggcatca gcgtgaatcg acacgaggaa attggcgttt tgcttacgtg
    13261 ccacatcgct gcgccccatc accgagataa agtaatcccc gtcacgggtt aaaacgcctt
    13321 taaacatcgg atcgtcattg agcaaagtac gcaatttacg cgcgatggcg atggtgacat
    13381 ttttctcccg cgtaccaccg gggccgatag cgccagggtc ctgaccgccg tgtccggcat
    13441 caatagcgat gataatttta tcgccattgt tagccgtcgc gcgcgctgcc ggacgcgtta
    13501 ccgtattact gctgataaca cccgtagtgc ggttactttc cgttttaaac ggattgcgcg
    13561 ccggttcgct gacgcgcggt gcgacaaccg taggcgtttc aacgcgtttc acaaccacag
    13621 gcggcggagg aggcggtggc ggcgcatcgg cgttaatcgt aaagacgaca gtgtaattgc
    13681 tgccattctg ccgcttcacc gcttcggttt taccattttc ggtaagatcg accaccagcc
    13741 gtagcgtttg tgcatcttta ggcgttccgg agcgaatcgc cttcaccaga ttattaccgc
    13801 tgaacaacaa cggcagtccc tgaatcacgc ccgtttgttt gatatcgagc gccacggtgc
    13861 gtttgctttg atggctaaac gcataatcag gatcgccaat aaaactcaac gttatccgcg
    13921 cctgttggtt gccgttagaa acctgaatat cagagagcgt cgcggcaccc acctgcgcgc
    13981 acagcagcag caacgtcgct accaaccaat ttctgatgcg atacatcatc ccaccacctt
    14041 tcaaaggtta accggctaaa cgtgccagca acaattcacc cgcagaggaa accgcactca
    14101 cgcgcgcctc acggccttgt gcctgataat caatgtgtat ttcgacatcc gggtcaggaa
    14161 gaacacctgt accttgttgt ggccactcca ccaggcagat ggcatcgttg gcaaaataat
    14221 cgcggatccc cataaactcc agctcctcgg gatcggcaag gcggtacaaa tcaaagtgat
    14281 aaaccattaa attgtcgagc gtatagggtt cgaccagcgt ataagtgggg cttttgacat
    14341 taccctgatg gcccaaagcc tgtaaaaagc cccggctaaa ggtggtttta cctgcgccta
    14401 aatcgccata cagatagatt acggttgcgc catcgcaggc tttcgctacc cgctcgccca
    14461 ggtctaatgt tgcctgctca tcagggagcg gaattactcg attcatcatg gtttttatca
    14521 gtcacttccg ggttaacaat acgctgtagc gtggaaaaga gatcggttgc cagcatcccg
    14581 cgcgttccaa aacgcgccgc cagtacgtca gctgccgcac cgtgcgcgac acagcctgca
    14641 caggctgcat catacggcga cagtttttgc ccaagcaatg cgccaataat accagagagc
    14701 acatcgccca tgccgccgct tgccatgcct gcatttccgg catcaataat gcctaaagcg
    14761 tcaggatggg cggcgaccac ggttccggca cctttcagca ccgctacgcc gccataacgt
    14821 tgtaccagac gtttggcgca atgtaagcgg tcactttcaa tttcagcgac ggaacagcct
    14881 aacaaccgtg cggcctcgcc aggatgcggc gtgatcacgc gattgtgacg cttatcggga
    14941 ttgattgcca gcaggttcaa tgcatcggca tcccacaaca tcggtttgcg aaaattctca
    15001 actttttgca gggctttttt cccccactct tgctggccca gaccgggacc aatcaccacc
    15061 acatcggccc attccaggct ttcggtaaga gagtccatag tcagttcatg caccatcaat
    15121 tctggtcgtg cagtcagcag cggcgcaatg ttctcactgc gggtcagtac tcggactaaa
    15181 ccagcaccag cacgtagcgc cgcttccccc gtcatacgaa tagcccccgc cgtgccgtga
    15241 tcgccaccga taatcaccag ccgcccgtga tcgcctttat gcgaagtcgg gcgacgcggt
    15301 ttcagccagt gagaaagttg ttctgccgaa aaccgctgaa ttttcgtctc ctgacctgcc
    15361 agccagctat ccagccccag tgagtcaaaa tgcagttgtc ctgtaacatc ccgcgctttt
    15421 ccagtgagca agcctggttt cagcgcaata aaagtgatgg tgtgatcggc gttgatcact
    15481 gcgcctggcg tagcgccggt ttcagccagc aggccggaag ggatatcaac cgccacaatc
    15541 ggcgcaggat gggaattagc gtggtcgatt aactggctaa tggattcgcg gggtgcttgc
    15601 tgcaagccgg taccgagcag cgcatcaaca atcagagcta ccgactcggg ccagacaata
    15661 ttcgaagcat ggatctcgcc tcccgcgttt aaccatgcct cgcgtgccag cgcggcctct
    15721 tccggcaacg gtttatcgct ctcctgggcc aacaacgtga cctcaatgcc gaccgctttg
    15781 gccagtcgcg cgaccacgta gccatcgccg ccgttattac catgaccgca caacaccagc
    15841 cagtggcggg cgtcaggata cgccgaacga cacacctgga atgcggcctc gccagcgcga
    15901 agcatcagct catagagtgt gagccccagc gcatctgccg cctcgcgttc tccgcggcgg
    15961 atatcgtcgg cgtgccagac ggtgtgtggt atacttacgg ggtttttctt cattgtatgg
    16021 tccgtcatgt cagagcccct cgatctcaat cagttagcgc aaaaaattaa acagtggggg
    16081 ctggaactgg gctttcagca ggtaggtatt accgataccg atctcagcga gtccgagccc
    16141 aaactgcaag catggctgga caaacaatac cacggcgaaa tggactggat ggcacgtcac
    16201 ggtatgctgc gcgctcgccc ccatgagtta ttgcccggta cgctgcgcgt gatcagcgtg
    16261 cggatgaatt accttcctgc taacgccgca tttgccagca cgctgaaaaa ccccaaactc
    16321 ggctatgtta gccgttatgc gctgggccgt gactatcaca aacgtctgcg caaccgactc
    16381 aaaaagctgg gcgagatgat tcagcaacat tgtgtttcgc tgaattttag accgtttgtc
    16441 gattctgcgc ctattctcga gcgcccgtta gctgaaaaag ctgggctcgg ctggacaggt
    16501 aagcactcac ttatcctcaa tcgcgaggcc ggttcgttct tctttttagg cgaattgctg
    16561 gtcgatattc cgctgcccgt ggatcaacca gtcgaggaag gatgcggcaa atgcgtggcc
    16621 tgtatgacga tttgcccgac cggtgccatc gtcgagccat ataccgtcga tgctcgccgc
    16681 tgtatctctt atctcaccat cgaactggaa ggggcgatcc cggaagagtt gcgaccgtta
    16741 atgggaaacc gtatttacgg ttgcgatgac tgccagctta tctgcccgtg gaatcgctat
    16801 tcacaactca ctacagaaga cgatttcagc ccgcgtaagc cgctacacgc accggaactc
    16861 attgagttat tcgcctggag cgaagagaag tttttaaaag tcacggaagg ttcggcgatt
    16921 cgccgtatag gtcacctgcg ctggctgcgt aatatcgccg tggcattagg caatgcgccc
    16981 tgggatgaaa cgattctggc ggcgctcgag agtcgcaaag gtgagcaccc acttcttgat
    17041 gagcacatag cgtgggcgat ggcgcagcaa atcgagaggc gaaatgcgtg catagtcgaa
    17101 gtgcaattgc cgaaaaaaca gcgtctggtt cgggtaattg aaaaagggtt accgcgtgac
    17161 gcctgattca tccacagctt gtgaataaaa ataaaaacgc attgcaattc aagacgaata
    17221 aaatcgtcaa gtgatcgact cgacaaaacg aaatcttaat ttttaaatta tctatcaata
    17281 ggttaaaaaa atactattca ccgtgcgaag tttctttgaa attaagatta cggcctgagg
    17341 ctgtggataa ctctgtttac aagagtttgt cagagacaac aaaaaccagc aataacgcat
    17401 cgcgtcgctg tggatatttt attgagagaa gaatttggag cgggaaacga gactcgaact
    17461 cgcgaccccg accttggcaa ggtcgtgctc taccaactga gctattcccg cttgggtggt
    17521 ctgtgcctta cagcactttc aaattttgga gcgggaaacg agactcgaac tcgcgacccc
    17581 gaccttggca aggtcgtgct ctaccaactg agctattccc gcttgggtgg tctgtgcttt
    17641 gcagcacttt tcaaattttg gagcgggaaa cgagactcga actcgcgacc ccgaccttgg
    17701 caaggtcgtg ctctaccaac tgagctattc ccgcgtacta cttaattttg ctttcgtaat
    17761 tttcgcattt ctgcgtcgtt acgggaggcg cattatacga gaaatctttc cctctgcaac
    17821 ccccctaaaa attttttttt gagtttttat ctcaagtgct gaaattatca gcaagacgtt
    17881 caatttagcg tcaaaatcac gccgccgcaa gtggattccg gcgcttacgt gaccagcaaa
    17941 aattttacag cttgataaaa tgctcgcggt agtaagccag ctccgccacc gattcacgga
    18001 tatcatccat cgcctgatgc gtcccctgtt tggtaaaacc atcaagaatt tccggcttcc
    18061 agcggcgcgc cagctctttc agggtgctga catcgagata acggtagtgg aagtaggctt
    18121 ccagctccgg catgtattta aacaggaagc gacgatcctg accgatgctg ttaccgcaaa
    18181 tcggcgattt acccgcaggc acccactgtt ttaaaaattc gagcgttgcc agttcagctt
    18241 cacgatcgcc catcgtgctc gctttcacgc gctccaccag cccgctggcg gtatgggtgc
    18301 gcacgttcca gccatccatc agcgccagct gttcatcgga ctgatgtact gcaatggtcg
    18361 gcccttctgc cagaatattc agattggcat cggtcaccag cgtggcaatc tcaataatgc
    18421 gatcgcgctc gggatccaga ccggtcatct caagatcgat ccaaatcagg ttgttttcat
    18481 tggcactcat gctattttcc acccattcac agtgactata ttgatccaca attgcgtgta
    18541 tcatagatgt tttgcccatc aggggcgacc aggagtcagt acgattgagt aaaaataaac
    18601 tctccaaagg ccagcagcgc cgcgtgaacg ccaatcacca gcgtcgtctt aaaacgtcta
    18661 aggagaagcc cgactacgac gacaatctgt ttggcgagcc tgatgaaggt atcgtcatca
    18721 gccgctttgg tatgcacgct gatgtggaat ccgccgatgg cgacgttcac cgctgcaata
    18781 ttcgccgtac catccgttcg ctggtgaccg gcgaccgcgt agtctggcgt ccgggtaaac
    18841 cggcggcgga aggcgtaaat gtcaaaggga tcgtggaagc ggtgcatgag cgcacctcag
    18901 tgttgacgcg tccggatttc tacgacggcg tgaaacctat tgccgccaac atcgaccaga
    18961 ttgtcattgt ctccgccatt ttgccggagc tgtcgctcaa tattatcgac cgttacctgg
    19021 tggcctgcga aaccttgcag attgagccga ttattgtgct caacaagata gacctgctgg
    19081 acgacgaagg catggcgttc gtcaacgaac agatggatat ctaccgcaat atcggttatc
    19141 gcgtattgat ggtttccagc catactcagg atgggctaaa accgctggaa gaggcgttga
    19201 ccgggcgcat cagcattttt gccgggcagt ctggcgtcgg caaatccagc ctgctgaacg
    19261 cgttgctggg gctgcaaaaa gagatcctga ccaacgatgt ctctgacaac tcggggcttg
    19321 gtcagcacac cacgaccgcc gcacgcctgt atcacttccc gcacggcggt gatgtgattg
    19381 attcccctgg tgtgcgtgag ttcggcctct ggcacctgga gccggaacaa atcactcagg
    19441 gctttgtcga attccatgac tatttaggtc tgtgtaaata tcgcgattgc aaacacgata
    19501 ccgatccggg ctgcgctatc cgggaagcgg ttgaggaagg aaaaatcgcg gaaacccgtt
    19561 tcgaaaacta tcaccgtatt ctggaaagca tggcgcaggt aaaaacgcgt aaaaactttt
    19621 ctgatacgga tgactgacaa ttaagctaac cgtcgttaga atcgtcccct ttttttcagg
    19681 atccggcatt taggccggat caggaacgac aaaacaatgg cctggaggct accttgttaa
    19741 attcatttaa actttcgcta cagtacattc tgccgaaact atggcttact cgcctggcgg
    19801 gttggggcgc aagcaagcgg gcaggatggc tgacaaaact ggttatcgat ctgttcgtta
    19861 aatactacaa ggtcgacatg aaagaggcgc aaaagccgga caccgccagc taccgcacct
    19921 ttaacgaatt ctttgtccgt ccgctgcgtg acgaagtacg cccaatcgat accgatccga
    19981 atgtcctggt catgcctgcc gatggcgtta tcagccagtt gggtaaaatc gaagaagata
    20041 aaatcctgca agccaaaggc cacaactaca gcctcgaagc cctgctggca ggcaactatc
    20101 tgatggcgga cctgttccgc aacggtacgt ttgtgaccac ttacctctcc ccgcgtgact
    20161 accaccgcgt acacatgccg tgcaacggta ttctgcgtga gatgatctac gtgccgggcg
    20221 atctcttctc cgttaaccat ctcacggctc agaacgtgcc gaatctgttt gcccgtaacg
    20281 aacgcgtgat ctgcctgttc gataccgaat ttggcccaat ggcgcagatt ctggtcgggg
    20341 cgacgattgt aggcagcatt gagacggtct gggcgggcac cattacgccg ccgcgcgaag
    20401 gcatcatcaa gcgctggacc tggcctgccg gggaaaacga cggttctgtg gcgctgctga
    20461 aaggccagga aatgggtcgc tttaaacttg gctctaccgt tatcaacctg tttgcaccgg
    20521 gtaaagtgaa tctggttgag caactggaaa gcctgtctgt cacgaaaatt ggccagccgc
    20581 tggcagtatc taccgaaacc tttgttacgc cagacgctga acccgccccg cttcctgctg
    20641 aagagatcga agcagaacac gacgccagcc cattggttga cgataaaaaa gaccaggtct
    20701 aatccatcaa aggaaacgct gacgtgcgcc tgattatcac ttttctgatg gcctggtgcc
    20761 tcagttgggg ggcgtacgcc gcgacggccc ccgatagcaa acaaatcacc caggaactgg
    20821 agcaggcaaa agcggcgaaa cccgcgcagc cagaagtcgt agaggcactc cagtctgcct
    20881 taaatgcgct tgaggaacga aaaggttccc ttgagcgcat caagcaatat caagaagtca
    20941 ttgataatta tccgaaactc tccgctactc tgcgcgcaca attaaacaac atgcgtgacg
    21001 agccgcgcag cgtgtcgccg gggatgtcta ccgacgcgct gaatcaggaa attctccagg
    21061 tcagcagtca gttgctggat aaaagccgtc aggcccagca agagcaggag cgcgcccgcg
    21121 agattgccga ttcgctgaat caactgccgc aacagcaaac cgacgcccgc cgtcagttaa
    21181 atgagatcga gcgccgcctg gggacgctta ccggcaatac tccgctcaat caggcacaaa
    21241 atttcgcgtt gcagtctgac tctgcacgtc ttaaggcgct cgttgatgaa ctggagctgg
    21301 cgcagctgtc tgccaataac cgccaggaat tagcgcgctt gcgctctgag ctggctgaaa
    21361 aagagagcca gcaactggat gcgtatttgc aggccttgcg taatcaattg aacagccaac
    21421 gccagcttga ggcagagcgg gcgctggaaa gtaccgaatt actagcagaa aacagtgccg
    21481 atttgccgaa agatatcgtc gcgcaattca aaattaaccg cgaactatcg gcggcgctga
    21541 atcaacaggc gcagcggatg gatctcgttg cctcgcaaca gcgtcaggct gccagccaga
    21601 cgttacaggt ccggcaggcg ttgaatacgc tgcgtgaaca gtcgcaatgg ctgggatcgt
    21661 ccaatctgct cggtgaagcg ttgcgggcgc aggtggcacg gctgccggaa atgccgaaac
    21721 cacaacagct tgataccgaa atggcgcagt tgcgtgtgca acggttacgt tatgaggatc
    21781 tgcttaataa acagccgctg ctacggcaaa ttcatcaggc cgacggtcag ccgctgaccg
    21841 ctgagcaaaa ccgtattctg gaagcacagc tacgcactca gcgtgagttg ctgaactcat
    21901 tgttgcaggg tggcgatacg ctactgctgg aactgaccaa gctgaaagtc tccaacgggc
    21961 aactggaaga tgcgctgaaa gaagtgaacg aagcgaccca ccgctatctg ttctggacct
    22021 ctgacgtgcg cccgatgacc atcgcctggc cgctggaaat cgcccaggat ctgcgtcgtc
    22081 tcatttcgct ggacaccttc agtcagttgg gcaaagccag tgtgatgatg ctgaccagca
    22141 aagagacgat tttgccgctg tttggcgcgt tgattctggt cggttgcagt atttactcgc
    22201 gccgctattt cacccgtttt cttgaacgtt cggcggcgaa agtcggcaaa gtgactcagg
    22261 atcacttctg gctgacgttg cgcactcttt tctggtcgat tctcgtcgcg tcaccgctgc
    22321 cggtgctgtg gatgacgctg ggttacggct tgcgcgaggc gtggccttat ccgctggcgg
    22381 tcgcgattgg tgatggcgtc acagccaccg tgccgctgct gtgggtagtg atgatttgcg
    22441 ctacctttgc ccgcccgaac ggcttgttta tcgctcattt tggctggccg cgcgaacgtg
    22501 tttctcgtgg gatgcgctac tacctgatga gcatcgggct tattgtgcca ctgattatgg
    22561 cgctgatgat gttcgataac ctcgacgacc gcgaattttc cggttcgctg ggacggcttt
    22621 gctttatcct catttgcggt gcgctggcgg tggtcaccct cagcctgaaa aaggccggga
    22681 tcccgctgta tctcaacaaa gaaggcagcg gcgacaatat taccaaccat atgttgtgga
    22741 acatgatgat tggcgcgcca ctggttgcca ttctggcgtc ggcggtgggt tatctggcaa
    22801 cggcacaggc gctgttagcg aggcttgaaa cctcggttgc catctggttc ctgctactgg
    22861 tggtttatca cgttatccgc cgctggatgc tgatccaacg acgcaggctg gcgtttgacc
    22921 gggcgaagca tcgccgggca gagatgttag cgcaacgtgc gcgtggcgaa gaggaagcac
    22981 atcatcacag tagcccggaa ggggcaattg aagtcgatga aagcgaagtc gatctcgatg
    23041 ccatcagtgc gcagtccttg cggctagtgc gctcaatttt gatgttgatc gccctgcttt
    23101 cggtcattgt gctgtggtca gaaatccatt ccgctttcgg cttcctcgaa aatatttcgc
    23161 tgtgggatgt cacctccacg gtacagggcg tagaaagtct ggagccgatt accctcggtg
    23221 cggtgctgat tgccattctg gtgtttatca tcaccacgca gctggtgcgc aatctgcccg
    23281 cgctgctgga actggcgatt ttgcagcacc tggatttaac gccgggtacc ggctacgcca
    23341 tcaccaccat caccaaatat ctgctgatgc tgattggcgg gctggtcggc ttctcgatga
    23401 ttggtattga gtggtcgaaa ttgcagtggc tggtcgccgc gctcggtgtt ggtctcggtt
    23461 ttggtttgca ggaaattttc gccaacttta tctctggcct gatcatcctg ttcgaaaaac
    23521 cgattcgcat tggcgatacg gtgacaattc gcgatcttac tggtagcgtg acgaaaatta
    23581 atacccgcgc aaccaccatc agcgactggg accgtaaaga gataatcgtg ccgaacaagg
    23641 cgtttattac cgagcagttt atcaactggt cgctctctga ctcggtcacg cgcgtggtgt
    23701 tgacgattcc ggcccctgcc gatgccaaca gtgaagaagt gacggaaatc ctgctcaccg
    23761 cagcgcgtcg ctgctcgctg gtgatcgaca acccggcacc ggaagtcttc ctggtggatc
    23821 tgcaacaggg gattcagatt ttcgagctgc gtatttacgc cgctgagatg ggtcaccgta
    23881 tgccgctacg ccatgagatc caccagctga ttctggctgg cttccatgcc cacggtatcg
    23941 atatgccatt cccgcccttc cagatgcgtc tggaaagcct caacggtaaa caaacgggga
    24001 gaacgctgac gtctgcgggc aaaggtcgtc aggcgggaag tttgtaatgc atgaagctgc
    24061 caaatgatgt caacgtggct catttagcag cttaacatca tcttcagtta tcaccggccc
    24121 tggagagtat catccatacg cattacttcc tgacaactca tcgccggaga gacgatgagt
    24181 tgcaggattt catatgatca gtcgagagct aactcaggtt tattcgccat gcgactgcaa
    24241 tagttctggt aaatcgccat cgctaacagc gagaagaaga cagggccgcc aatcatccac
    24301 aatgtgctgt cccagtctcc ggcttcaacc acaggctgaa taatggtgaa gacgttcgca
    24361 aatgtcacca ccagaacgac aaccaccgtc gcaatcattg ccgacatacg cgttttgaaa
    24421 atcacaaatg gtctgtcgag atcctgacgt gctttaaaga atgggaaagc cagcgcgagg
    24481 aacaggtaag gaagcgtcat agacacgttc gccatcagcg tcagcttgtt aaagaacgcc
    24541 gatgcggtac cgccaccaaa cgaaaccagc aggatgaaga cagtaaccaa cccgcactgc
    24601 atccacatag cgatagacgg catccccatc gcattcaggc gcgtcatcgg ttccggccac
    24661 aacgctttcg gcgtcccctg gatgatggct ttcagcggtg aatagcaaag cgtaaagaac
    24721 gcaccggtat aagcgaggaa catcgaaagt ccggtaatac gcgcaaacca tacgcccagc
    24781 gacaatgacg cttcaggtga caaatgcagt gcgttaccca gcgtcacccc gaggctcttc
    24841 atcagcacat aggtaatatt gccgaggtta acggaaccat tacttaatac ctgctgccag
    24901 tttgtgctga cgccccataa aaatattgcc agcgaataac cgattgaaat aacaatagcg
    24961 gcaaaaacaa tacctttggc aaagttcttt tctggatttt ccgttttatc gaccagacca
    25021 ccaaccgctt caattccgcc ataggcaaaa atagcaaata ccacaaacga tagcattgcc
    25081 agaccggact gataacctgg gttcggtgat gcaaggaaat taatatcctg cgcgaaatgc
    25141 ccaccattta ataacaaaat agtaatgctt actaacagca atactaaatt cagacacatt
    25201 actgcaatac cgcccaccgc agtaatgcgg gcaattttat taatcccctt tgaagcaacg
    25261 acggtgacca gaatcatcca tgccactgcc agcagaccaa ccacctgcgt aggctccagt
    25321 ccggcaatac gccagtgctg ggtcatgtcg ctaccataga ggaatgttga gaacggtacc
    25381 caaactttcg cggaggtgct caccatccag atgatataag aggaaaacca cataaacgta
    25441 ccaatgaagg caaaacgtgg tccgacacta ttattcatcc aggaatagat accgccttct
    25501 tctttgcgat aagcagctcc catttcagcc atcattaagg cgaatggaat aaagaataac
    25561 aatgcagaaa atatataaaa gggaatcgca ctataaccca ttaagtaata agccgatggg
    25621 ctattggcaa atccaaatac ggaagtaaag atcatcaata tgagtcctat cagactcatc
    25681 ttttttatcg tgtgaggcat gaaaccatcc ttcatttcag cgcgtgtata aaacgcgccc
    25741 actgaactgc actgcccaac attgagcggg cagcgtaaaa accaatacag aattgatacc
    25801 tggatgatat caaatatcca gtcttaaatt gtggccttta gcggaaaaat gaaaacatat
    25861 gctacagaat ggcgcttatc acgccattct tcgctgttaa ttcagtaatt tttcagaatt
    25921 atgcccggtc aacgctaaag gcgatgactt cagccagtgt ctccgcgccc agcgccaaca
    25981 tcaccagacg atcaacacct aatgccacgc cggaacagtc aggcataccg actttcaagg
    26041 cttcaatcag attctgatca atggggtgct gcggcaaacc gcgcgccgcg cgcttacggt
    26101 tatcttgttc aaagcgttgt tgctgctcac gggcatccgt caattcatgg aaaccattcg
    26161 ccagctcaat acctttataa taaacctcaa agcgttcagc gacccgatga tcttcggtac
    26221 tgatttgcgc cagtgatgcc tggctggctg gaaagtggta cacaaaggtc ggtttttctt
    26281 taccaatatt tggttctacg ccaaaggtaa acagcaattg tagcaacgtg tcgcggtctt
    26341 cttcggtatc agcaacattg ctcaaatcca gtttcgctgc cacttcccgc agttgcgttt
    26401 tgtcggcaga gagcgggtca atttccagat aacgcaagaa agcttgttga taagaaaggc
    26461 tttctgctgc cgggcagtcc agcacctgtt gtaagagatc gtccacctcg ttcatcaacc
    26521 ggtacatatc atagtgcggt cgataccact caagcatagt gaactcaggg ttgtgataac
    26581 gccccatctc ttcattacgg aaactgcggc acagctggaa taccggcccg caaccggcaa
    26641 ccagcaggcg tttcatatgg tattccgggc tggtcattaa ccagagattc atcccctgcg
    26701 aatgcccggg gccaacgaaa cgtgtctcaa acgggaccaa atgaatatcg gttaccgtcg
    26761 cctggctcat acaaggcgtc tccacctcca gcactccacg atcggcaaag aaacgacgga
    26821 tctccgccat aatcgccgcg cgttttaata agttaggaat ggatgcgctc ggctgccagg
    26881 atgccgtttc gctcatagtt aaatctccag tttttgacaa gggcacgaag tctactcgca
    26941 acgcgacggc gagacaaatt ttacgcagga ataaaacaac ggtgggcagt gactaaaaaa
    27001 agcacgatct gatggtttag taattaaatt aatcatcttc agtgataatt tagccctctt
    27061 gcgcactaaa aaaatcgatc tcgtcaaatt tcagacttat ccatcagact atactgttgt
    27121 acctataaag gagcagtgga atagcgttcg cagaccgtaa ctttcaggca cttaccctga
    27181 agtacggggc tgtgggataa aaacaatctg gaggaatgtc gtgcaaacct ttcaagccga
    27241 tcttgccatt gtaggcgccg gtggcgcggg attacgtgct gcaattgctg ccgcgcaggc
    27301 aaatccaaat gcaaaaatcg cactaatctc aaaagtatac ccgatgcgta gccataccgt
    27361 tgctgcagaa gggggctccg ccgctgtcgc gcaggatcat gacagcttcg aatatcactt
    27421 tcacgataca gtagcgggtg gcgactggtt gtgtgagcag gatgtcgtgg attatttcgt
    27481 ccaccactgc ccaaccgaaa tgacccaact ggaactgtgg gggtgcccat ggagccgtcg
    27541 cccggatggt agcgtcaacg tacgtcgctt cggcggcatg aaaatcgagc gtacctggtt
    27601 cgccgccgat aagaccggct tccatatgct gcacacgctg ttccagacct ctctgcaatt
    27661 cccgcagatc cagcgttttg acgaacattt cgtgctggat attctggttg atgatggtca
    27721 tgttcgcggc ctggtagcaa tgaacatgat ggaaggcacg ctggtgcaga tccgtgctaa
    27781 cgcggtcgtt atggctactg gcggtgcggg tcgcgtttat cgttacaaca ccaacggcgg
    27841 catcgttacc ggtgacggta tgggtatggc gctaagccac ggcgttccgc tgcgtgacat
    27901 ggaattcgtt cagtatcatc caaccggtct gccaggttcc ggtatcctga tgaccgaagg
    27961 ttgccgcggt gaaggcggta ttctggtcaa caaaaatggc taccgttatc tgcaagatta
    28021 cggcatgggc ccggaaactc cgctgggcga gccgaaaaac aaatatatgg aactgggtcc
    28081 acgcgacaaa gtttctcagg ccttctggca cgaatggcgt aaaggcaaca ccatctccac
    28141 gccgcgtggt gatgtggttt atctcgacct gcgtcacctc ggcgagaaaa aactgcatga
    28201 acgtctgccg ttcatctgcg aactggcgaa agcgtacgtt ggcgtcgatc cggttaaaga
    28261 accgattccg gtacgtccga ccgcacacta caccatgggc ggtatcgaaa ccgatcagaa
    28321 ctgtgaaacc cgcattaaag gtctgttcgc cgtgggtgaa tgttcctctg ttggtctgca
    28381 cggtgcaaac cgtctgggct ccaactccct ggcggaactg gtggtcttcg gccgtctggc
    28441 cggtgaacaa gcgacagagc gtgcagcaac tgccggtaat ggcaacgaag cggcaattga
    28501 agcgcaggca gctggcgttg aacaacgtct gaaagatctg gttaaccagg atggcggcga
    28561 aaactgggcg aagatccgcg acgaaatggg cctggctatg gaagaaggtt gcggtatcta
    28621 ccgtacgccg gaactgatgc agaaaaccat cgacaagctg gcagagctgc aggaacgctt
    28681 caagcgcgtg cgcatcaccg acacttccag cgtgttcaac accgacctgc tctacaccat
    28741 tgaactgggc cacggtctga acgttgctga atgtatggcg cactccgcaa tggcacgtaa
    28801 agagtcccgc ggcgcacacc agcgtctgga cgaaggttgc accaagcgtg acgacgtcaa
    28861 cttcctcaaa cacaccctcg ccttccgcga tgctgatggc acgactcgcc tggagtacag
    28921 cgacgtgaag attactacgc taccgccagc taaacgcgtt tacggtggcg aagcggatgc
    28981 agccgataag gcggaagcag ccaataagaa ggagaaggcg aatggctgag atgaaaaacc
    29041 tgaaaattga ggtggtgcgc tataacccgg aagtcgatac cgcaccgcat agcgcattct
    29101 atgaagtgcc ttatgacgca actacctcat tactggatgc gctgggctac atcaaagaca
    29161 acctggcacc ggacctgagc taccgctggt cctgccgtat ggcgatttgt ggttcctgcg
    29221 gcatgatggt taacaacgtg ccaaaactgg catgtaaaac cttcctgcgt gattacaccg
    29281 acggtatgaa ggttgaagcg ttagctaact tcccgattga acgcgatctg gtggtcgata
    29341 tgacccactt catcgaaagt ctggaagcga tcaaaccgta catcatcggc aactcccgca
    29401 ccgcggatca gggtactaac atccagaccc cggcgcagat ggcgaagtat caccagttct
    29461 ccggttgcat caactgtggt ctgtgctacg ccgcgtgccc gcagtttggc ctgaacccag
    29521 agttcatcgg tccggctgcc attacgctgg cgcatcgtta taacgaagat agccgcgacc
    29581 acggtaagaa ggagcgtatg gcgcagttga acagccagaa cggcgtatgg agctgtactt
    29641 tcgtgggcta ctgctccgaa gtctgcccga aacacgtcga tccggctgcg gccattcaac
    29701 agggcaaagt agaaagttcg aaagactttc ttatcgcgac cctgaaacca cgctaaggag
    29761 tgcaacatga cgactaaacg taaaccgtat gtacggccaa tgacgtccac ctggtggaaa
    29821 aaattgccgt tttatcgctt ttacatgctg cgcgaaggca cggcggttcc ggctgtgtgg
    29881 ttcagcattg aactgatttt cgggctgttt gccctgaaaa atggcccgga agcctgggcg
    29941 ggattcgtcg actttttaca aaacccggtt attgtgatca ttaacctgat cactctggcg
    30001 gcagctctgc tgcacaccaa aacctggttt gaactggcac cgaaagcggc caatatcatt
    30061 gtaaaagacg aaaaaatggg accagagcca attatcaaaa gtctctgggc ggtaactgtg
    30121 gttgccacca tcgtaatcct gtttgttgcc ctgtactggt aaggagcctg agatgattaa
    30181 tccaaatcca aagcgttctg acgaaccggt attctggggc ctcttcgggg ccggtggtat
    30241 gtggagcgca attattgcgc cggtgatgat cctgctggtg ggtattctgc tgccgctggg
    30301 gctgtttccg ggtgatgcac tgagctacga gcgcgttctg gcgttcgcgc agagcttcat
    30361 tggtcgcgta ttcctgttcc tgatgatcgt tctgccgctg tggtgtggtt tacaccgtat
    30421 gcaccacgcg atgcacgatc tgaaaatcca cgtacctgcg ggcaaatggg ttttctacgg
    30481 tctggctgct atcctgacag ttgtcacgct gattggtgtc gttacaatct aacgcatcgc
    30541 caatgtaaat ccggcccgcc tatggcgggc cgttttgtat ggaaaccaga ccctatgttc
    30601 aaaacgacgc tctgcgcctt attaattacc gcctcttgct ccacatttgc tgcccctcaa
    30661 caaatcaacg atattgtgca tcgcacaatt accccgctta tagagcaaca aaagatcccc
    30721 ggtatggcgg tggcggtaat ttatcagggt aaaccttatt actttacctg ggggtatgcg
    30781 gacatcgcca aaaagcagcc cgtcacacag caaacgttgt ttgagttagg ttcggtcagc
    30841 aaaacattta cgggcgtgct tggtggcgac gctattgctc gaggggaaat caagttaagc
    30901 gatcccgcaa caaaatactg gcctgaactt accgctaaac agtggaatgg gatcacacta
    30961 ttacatctcg caacctacac tgctggcggc ctgccattgc aggtgccgga tgaagtgaaa
    31021 tcctcaagcg acttgctgcg cttctatcaa aactggcagc ctgcatgggc tccaggaaca
    31081 caacgtctgt atgccaactc cagtatcggt ttgttcggcg cactggctgt gaagccgtct
    31141 ggtttgagtt ttgagcaggc gatgcaaact cgtgtcttcc agccactcaa actcaaccat
    31201 acgtggatta atgtaccgcc cgcagaagaa aagaattacg cctggggata tcgcgaaggc
    31261 aaggcagttc atgtttcgcc aggggcgtta gatgctgaaa cttatggtgt gaagtcgacc
    31321 attgaagata tggcctgctg ggtacgaagc aatatgaatc cccgtgatat caacgacaaa
    31381 acacttcagc aagggataca actggcacaa tctcgctact ggcaaaccgg cgatatgtat
    31441 cagggtctgg gctgggaaat gctggactgg ccggtaaatc ctgacatcat cgttaacggc
    31501 agtgacaata aaattgcact ggcagcacgc cccgtaaaag cgattacgcc tccaactcct
    31561 gcagtacgag catcatgggt gcataaaacg ggggcgaccg gcggatttgg tagctatgtc
    31621 gcgtttattc cagaaaaaga gctgggtatc gtgatgctgg ctaacaaaaa ctatcccaat
    31681 ccagcgagag tcgccgccgc ctggcagatt ctcaacgctc tacagtaaaa tttcatcggg
    31741 tccgaatttt cggacctttt ctgcgctttt ccttgctgtc atctacactt agaaaaaacc
    31801 agtaaggaaa catttatgcg cctgctccct ctcgttgccg cagcgacagc tgcatttctg
    31861 gtcgttgcct gcagttctcc tacgccgccg cgtggcgtga ccgtagtgaa taatttcgac
    31921 gctaaacgct atctcggtac ctggtatgag attgcccgtt ttgatcaccg ctttgaacgt
    31981 ggactggaaa aagtcaccgc aacatacagc ctgcgtgatg acggcggcct gaatgtcatt
    32041 aataaaggct ataaccctga cagaggaatg tggcagcaga gtgaagggaa agcgtacttt
    32101 accggcgcac caactcgcgc tgcgctgaaa gtgtcattct ttggtccttt ctatggcggt
    32161 tataacgtta ttgcactcga tcgggaatac cgccatgcgc tggtttgcgg accggaccgc
    32221 gactacctgt ggatactctc ccgcacgcca accatttctg acgaagtgaa acaggagatg
    32281 ctggcagtcg cgacccggga agggtttgat gtcagtaagt ttatttgggt acagcagccc
    32341 ggtagttagt gagtgctgag tttcagacca ataatcccca atacgattaa cgccagactc
    32401 gccagacgca tcgggttagc ggactcaccg agcagcacaa tgccggtgat ggccgcgccg
    32461 actgcgccaa tacccgtcca cacggcataa gccgtcccta ctggtaacga tttcatcgcc
    32521 caggcaagta gcgccatact gacaatcatc gccgtcacgg taataacact cggcgtcaaa
    32581 cgactaaagc cgtgggtata tttcaggcct acggcccata ccacttccag cagaccagca
    32641 ataactaaga taatccagga catatcaggc tccggagaat tggggccgtc cccggtgaaa
    32701 agacgcgttt gcaggtcgtc ctgcaaagct gatggttgaa cggatatttt gccaggtggc
    32761 gggggaattt caactattac ctttgtgact atcgcagaaa aaacaaacgg cacgacacag
    32821 ctgtcgtacc gttattgctt attgctgcgc tttcgttgct gcaccagaaa tcgcgttacc
    32881 gccatcagaa atgtcttcac caacgccacg cgtggtgttg caggcagtta atactgttga
    32941 aagcaccaga acagaaaaga tcgctgcaat tgtcttcttc accataacgt cttcctttta
    33001 ggtaatgtta tttatgtttg cctatagcaa attaataata gacaacatcg ccaaaaatga
    33061 tggaaatctg atgatttttc ggaagagaag aaaaattagc tggcagcgcg ggagatggag
    33121 ttgccgagat gtttgatgtc ttcgccgaaa ccgcgagcgg tgttacagcc cgtgagcagc
    33181 gtgctggcaa gcaaaacaag aacgataagg cgtttcatca tctctgacct gaataagtga
    33241 tggtgcagcc tgcaggccgc accacaaccg cattacttca cgcgagagac gtattcacca
    33301 gagcgggtat ccactttgat gacttcgccg atttgtacga acagcggaac tttaaccaca
    33361 gcgccagtag acagggtagc cggtttgccg ccagtacctg cggtatcacc tttcaggccc
    33421 ggatcggtat caacgatttc cagttcaacg aagttcggcg gagtaacgga gatcggctga
    33481 ccattccaca gagttacgat acactctgcc tgatccagca gccatttagc gttgtcgcca
    33541 attgcttttg cgtcagcaga cagctgctcg aaagtttcgt tgttcatgaa gtgccagaac
    33601 tcaccgtcgt tgtacaggta agtcaggttc atatcgacaa catcagcgcc ttcagcggaa
    33661 tcagtagatt tgaaggtttt ttctacgcga gtaccggtca gcagacgacg cagtttaacg
    33721 cgagcaaatg cctggccttt acccggtttt acgaattcac tcgcttcaac cgcgtaaggt
    33781 tcgccgtcta acatgatttt aagaccagca cgaaaatcgt tgctatagta cgttgccata
    33841 aggccctctg aaatttgtta attggtagct aagccacaaa atggcgcata ttgtaaccct
    33901 aaatacccca tccagagaag attggttaac gcaacttgcc gatgttgtga ccgatcctga
    33961 tgaacttctg cgtcttttga atatagacgc ggacgaaaaa ctgttggccg gacgcaacgc
    34021 caaaaagctg tttgccctgc gtgtgccccg ctcatttatc gatcgcatgg agaaaggcaa
    34081 tccggacgat ccgcttttgc gtcaggtact tacctcgcaa gatgagtttg tcgtcgcgcc
    34141 cggattctcc accgacccgc tggaagaaca gcacagcgta gtgcctggtt tgttgcataa
    34201 ataccacaac cgggcgcttt tgctggtcaa aggcggctgc gcggtaaatt gccgctattg
    34261 cttccgtcgc cacttcccct atgccgaaaa tcagggcaac aagcgtaact ggcaaactgc
    34321 acttgagtat gttgctgcgc atccggaact ggacgagatg attttctccg gcggcgatcc
    34381 gctgatggcg aaagatcacg agctggactg gttgctcaca caactggaag ccatcccgca
    34441 tatcaaacgt ctgcggattc acagtcgtct gccgattgtg atcccggcac gcatcacaga
    34501 tgcgctggtt gaacgctttt cacattctac gctgcaaatc ttgctggtga atcacatcaa
    34561 ccatgccaat gagatagatg aaacattccg tcaggcgatg gctaagttgc gccgggcggg
    34621 cgttactttg ctgaaccaga gcgttctgtt acgtggtgtg aacgataacg cacaaacgct
    34681 ggcaaacctg agtaatgcgt tgttcgatgc aggcgtgatg ccgtattacc tgcatgtgct
    34741 cgataaagta cagggcgcgg cgcattttat ggtgagtgat gacgaagcac ggcagattat
    34801 gcgtgagttg ctgacactgg tgtcgggata tctggtgccg aaactggcgc gagaaattgg
    34861 cggcgaaccc agcaaaacgc cgctggatct ccagctacgc cagcagtaac tgattaaaca
    34921 gggaagttta atctttgccc gttacataac ctttaattat tcatatacat tgctgttgtt
    34981 actctttata taaccaacac c
//
LOCUS       DBFIFM010000024        34881 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.024, whole genome shotgun sequence.
ACCESSION   DBFIFM010000024 DBFIFM010000000
VERSION     DBFIFM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.024"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..105
                     /locus_tag="N7C74_001072"
     CDS             <1..105
                     /locus_tag="N7C74_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2430989.1"
                     /translation="DGDQTLTFKVDYIATGKATSGNVNAVTNFHINYY"
     gene            188..889
                     /gene="elfD"
                     /locus_tag="N7C74_001073"
     CDS             188..889
                     /gene="elfD"
                     /locus_tag="N7C74_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU2430990.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSTWAAGKGGVGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKLVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            914..3513
                     /gene="elfC"
                     /locus_tag="N7C74_001074"
                     /pseudo
     CDS             914..3513
                     /gene="elfC"
                     /locus_tag="N7C74_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3504..4574
                     /gene="elfG"
                     /locus_tag="N7C74_001075"
                     /pseudo
     CDS             3504..4574
                     /gene="elfG"
                     /locus_tag="N7C74_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4602..5129
                     /gene="ycbU"
                     /locus_tag="N7C74_001076"
     CDS             4602..5129
                     /gene="ycbU"
                     /locus_tag="N7C74_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2430991.1"
                     /translation="MFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDVATKSA
                     LQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAPTLLKL
                     TDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKSTKAGAT
                     GGNATAVLYFDLVYQ"
     gene            5137..5652
                     /gene="ycbV"
                     /locus_tag="N7C74_001077"
     CDS             5137..5652
                     /gene="ycbV"
                     /locus_tag="N7C74_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2430992.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5618..6354
                     /gene="ycbF"
                     /locus_tag="N7C74_001078"
                     /pseudo
     CDS             5618..6354
                     /gene="ycbF"
                     /locus_tag="N7C74_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            6465..7475
                     /gene="pyrD"
                     /locus_tag="N7C74_001079"
     CDS             6465..7475
                     /gene="pyrD"
                     /locus_tag="N7C74_001079"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HGU2430993.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7649..8191
                     /gene="zapC"
                     /locus_tag="N7C74_001080"
     CDS             7649..8191
                     /gene="zapC"
                     /locus_tag="N7C74_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HGU2430994.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTNEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8188..9297)
                     /gene="ycbX"
                     /locus_tag="N7C74_001081"
     CDS             complement(8188..9297)
                     /gene="ycbX"
                     /locus_tag="N7C74_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HGU2430995.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDSSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PEAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILTTAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            9541..11649
                     /gene="rlmKL"
                     /locus_tag="N7C74_001082"
     CDS             9541..11649
                     /gene="rlmKL"
                     /locus_tag="N7C74_001082"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HGU2430996.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKEPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11661..13568
                     /gene="uup"
                     /locus_tag="N7C74_001083"
     CDS             11661..13568
                     /gene="uup"
                     /locus_tag="N7C74_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2430997.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKNEEPAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            13698..14951
                     /gene="pqiA"
                     /locus_tag="N7C74_001084"
     CDS             13698..14951
                     /gene="pqiA"
                     /locus_tag="N7C74_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HGU2430998.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14956..16596
                     /gene="pqiB"
                     /locus_tag="N7C74_001085"
     CDS             14956..16596
                     /gene="pqiB"
                     /locus_tag="N7C74_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HGU2430999.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     VLDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16593..17156
                     /gene="pqiC"
                     /locus_tag="N7C74_001086"
     CDS             16593..17156
                     /gene="pqiC"
                     /locus_tag="N7C74_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HGU2431000.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            17412..17579
                     /gene="rmf"
                     /locus_tag="N7C74_001087"
     CDS             17412..17579
                     /gene="rmf"
                     /locus_tag="N7C74_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HGU2431001.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17649..18167)
                     /gene="fabA"
                     /locus_tag="N7C74_001088"
     CDS             complement(17649..18167)
                     /gene="fabA"
                     /locus_tag="N7C74_001088"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HGU2431002.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18236..19996)
                     /gene="ycbZ"
                     /locus_tag="N7C74_001089"
     CDS             complement(18236..19996)
                     /gene="ycbZ"
                     /locus_tag="N7C74_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGU2431003.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20182..20634
                     /gene="matP"
                     /locus_tag="N7C74_001090"
     CDS             20182..20634
                     /gene="matP"
                     /locus_tag="N7C74_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HGU2431004.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20709..21749)
                     /gene="ompA"
                     /locus_tag="N7C74_001091"
     CDS             complement(20709..21749)
                     /gene="ompA"
                     /locus_tag="N7C74_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HGU2431005.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22106..22615)
                     /gene="sulA"
                     /locus_tag="N7C74_001092"
     CDS             complement(22106..22615)
                     /gene="sulA"
                     /locus_tag="N7C74_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HGU2431006.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            22834..23463
                     /gene="sxy"
                     /locus_tag="N7C74_001093"
     CDS             22834..23463
                     /gene="sxy"
                     /locus_tag="N7C74_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HGU2431007.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKYPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23426..25588)
                     /gene="yccS"
                     /locus_tag="N7C74_001094"
     CDS             complement(23426..25588)
                     /gene="yccS"
                     /locus_tag="N7C74_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HGU2431008.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(25598..26044)
                     /gene="yccF"
                     /locus_tag="N7C74_001095"
     CDS             complement(25598..26044)
                     /gene="yccF"
                     /locus_tag="N7C74_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HGU2431009.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIALIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26167..28221
                     /gene="helD"
                     /locus_tag="N7C74_001096"
     CDS             26167..28221
                     /gene="helD"
                     /locus_tag="N7C74_001096"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HGU2431010.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVLIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28253..28711)
                     /gene="mgsA"
                     /locus_tag="N7C74_001097"
     CDS             complement(28253..28711)
                     /gene="mgsA"
                     /locus_tag="N7C74_001097"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HGU2431011.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28807..29469)
                     /gene="yccT"
                     /locus_tag="N7C74_001098"
     CDS             complement(28807..29469)
                     /gene="yccT"
                     /locus_tag="N7C74_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HGU2431012.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29642..30055
                     /gene="yccU"
                     /locus_tag="N7C74_001099"
     CDS             29642..30055
                     /gene="yccU"
                     /locus_tag="N7C74_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HGU2431013.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(30100..30417)
                     /gene="hspQ"
                     /locus_tag="N7C74_001100"
     CDS             complement(30100..30417)
                     /gene="hspQ"
                     /locus_tag="N7C74_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HGU2431014.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30475..31665)
                     /gene="rlmI"
                     /locus_tag="N7C74_001101"
     CDS             complement(30475..31665)
                     /gene="rlmI"
                     /locus_tag="N7C74_001101"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HGU2431015.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            31760..32038
                     /gene="yccX"
                     /locus_tag="N7C74_001102"
     CDS             31760..32038
                     /gene="yccX"
                     /locus_tag="N7C74_001102"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HGU2431016.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(32035..32364)
                     /gene="tusE"
                     /locus_tag="N7C74_001103"
     CDS             complement(32035..32364)
                     /gene="tusE"
                     /locus_tag="N7C74_001103"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HGU2431017.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32455..33114)
                     /gene="yccA"
                     /locus_tag="N7C74_001104"
     CDS             complement(32455..33114)
                     /gene="yccA"
                     /locus_tag="N7C74_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HGU2431018.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKMANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(33522..34528)
                     /locus_tag="N7C74_001105"
                     /pseudo
     CDS             complement(33522..34528)
                     /locus_tag="N7C74_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(34506..34748)
                     /locus_tag="N7C74_001106"
     CDS             complement(34506..34748)
                     /locus_tag="N7C74_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HGU2431019.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
BASE COUNT         8967 a         8315 c         9049 g         8550 t
ORIGIN      
        1 gatggagatc agacgttgac ttttaaagtt gattatatcg ctacggggaa agcgacttct
       61 ggtaatgtga atgcggtaac aaatttccat attaactatt attaatagaa ctcattaatt
      121 gttttattaa ttagtacccc tccagtgtta tggaggggat attcatattt tttaagagtg
      181 actatttatg aaaacttgca taacaaaggg aattgtgacc gtaagtttaa cggcaatatt
      241 actcagctgt tcgtccacat gggcggcagg taaaggcggg gttggacttg cagcaacacg
      301 tcttgtttat tcggaaggtg aagagcaaat ttcactgggt gtacgtaaca ccagtccgga
      361 tgttccttat ctgattcagt catgggtgat gaccccagat aataaaaaat cagcggactt
      421 tattattaca ccaccgctat ttgtgctgaa tccggcaaac gagaatctgt tacgcattat
      481 gtacattggc gcgccgttgg cgaaagacag agaaactctt ttcttcacta gcgtacgggc
      541 agtcccttca acaacgaagc gggaagaagg caataccctg aagattgcca cacaaagcgt
      601 catcaaactt ttctggcgac caaaaggttt agcgtatccc ttaggcgagg ctccggcgaa
      661 actgcgttgc acttcgtcag ctgacatggt tacggtcagt aacccaacac cttatttcat
      721 taccctgaca gacctgaaaa taggtggaaa gttagttaaa aatcaaatga tttccccctt
      781 tgataaatac caattctctc tgccaaaggg tgccaaaaat agcagcgtaa cgtatcgaac
      841 catcaatgac tacggggcgg aaacgccgca actcaactgt aaatcgtaat tcgtcttcag
      901 ttaagagagc gagatgtata gaactcaccg acaacacagc ctgttaagct ctggtggagt
      961 gccatcgttt attggtgggc tggtggtgtt tgtgtcggca gcgttcaatg cacaagctga
     1021 aacctggttc gatccagcct ttttcaaaga tgatccctca atggtggccg atttgtctcg
     1081 tttcgaaaaa ggacaaaaaa taacgccagg ggtttatcgt gtcgatattg ttctgaatca
     1141 gacaattgta gatacgcgca acgtcaattt tgttgagata acgccagaga aggggattgc
     1201 cgcctgtttg acgactgaaa gcctggatgc aatgggcgtg aatactgatg catttccggc
     1261 ttttaaacaa ctggacaaac aagcgtgtgc gccattggcg gagattattc cggatgccag
     1321 cgtaactttt aatgtgaata aactccgtct ggaaatttca gtaccgcaaa ttgccatcaa
     1381 aagtaatgct cgtggttatg tccccctgaa cgttgggatg aagggatcaa cgcgctatta
     1441 ctgggatatt catttagcgg ggctaacagt attcatagca gcgcagacag tgattctggc
     1501 gacagttatt ttctgaattt aaacagtggc gttaatttag gcccatggag attgcgcaac
     1561 aattcaacat ggagtcgtag tagtggccaa accgcagaat ggaagaatct cagcagctat
     1621 ttgcaacggg cggttattcc tctgaaaggc gaactgaccg taggtgatga ttatactgca
     1681 ggcgattttt tcgatagtgt cagctttcgt ggtgtgcagc tggcgtcaga tgacaacatg
     1741 ctgccagaca gcctgaaagg gtttgcgcct gtggtgcgtg gtatcgccaa aagcaatgcc
     1801 cagataacga ttaagcaaaa tggttacacc atttaccaaa cttatgtatc gcctggtgct
     1861 tttgaaatta gtgatatcta ttccacgtcg tcgagcggtg atttgttagt tgaaatcaaa
     1921 gaagcggacg gcagcgtcaa tagctacagc gtaccctttt ccagcgtgcc attactccag
     1981 cgtcaggggc gaatcaaata cgcggtgaca ctggcgaaat acagaaccaa tagtaatgaa
     2041 cagcaggaga gcaaatttgc ccaggccacg ttgcagtggg gcggaccgtg gggaacgacc
     2101 tggtatggtg gtggacaata tgctgaatat taccgtgccg ccatgtttgg tctgggattt
     2161 aacctcggcg atttcggagc aatttcgttc gatgtgaccc aggcgaagag tacgctggca
     2221 gaccaaagcg aacataaagg tcagtcatat cgttttctgt atgccaaaac gctcaaccaa
     2281 ttgggtacta actttcaatt gatgggctat cgctattcga cgtcgggttt ctacaccctt
     2341 tccgacacca tgtataaaca tatggatggc tacgaattta atgacggtga tgatgaagat
     2401 acgccgatgt ggtcgcgtta ttacaatttg ttttacacca aacgtggcaa actgcaggtc
     2461 aatatctccc agcaattaag cgagtacggt tcgttttatt taagtggtag ccagcaaact
     2521 tactggcata ccgatcaaca ggatcggcta ttacagtttg gctataacac gcaaattaaa
     2581 gatctctcgc tggggatctc ctggaactac agtaagtccc gtggccaacc tgatgctgat
     2641 caggtgtttg cacttaattt ttccctgcca ctcaatctgt tgctctccag aagtaatgat
     2701 agctatacca gcaaaaaaaa ttacgcctgg atgacctcta acaccagtat cgataacgaa
     2761 gggcacacta cacaaaacct gggtttaacg gagacactac tcgatgacgg taacctgagc
     2821 tacagcgtgc aacagggata taacagcgag gggaaaacgg ctaatggcag cgccagcatg
     2881 gactacaaag gggtgtttgc agatgcccga gtgggctaca actacagcga taacggcagt
     2941 caacaacaac tgaactacgc tctttcaggc agtttagttg cccattcaca gggcattacc
     3001 ctggggcaat cactggggga aactaacgtt ctgattgcag caccaggcgc agagaatact
     3061 cgtgtggcga acagcaccgg gctgaaaact gactggcgcg gatataccgt tgttccttat
     3121 gccacttctt atcgtgaaaa tcgaatcgca cttgatgcgg cgtcgttaaa acgtaacgtg
     3181 gatcttgaaa atgcagtagt caacgtggtt cccaccaaag gggcgttggt tctggcggag
     3241 ttcaatgccc atgcgggagc aagggtatta atgaaaacat caaagcaggg tatatcgctg
     3301 cgttttggcg cgatagcgac gctggacggc gtacagacta atagcggcat aattgatgat
     3361 gatggctcgc tctatatggc gggtttaccg gcgaagggaa caataaccgt gcgctggggc
     3421 gaagctcccg atcaaatttg tcatatcagt tacgagctta ccgaacaaca aattaactct
     3481 gcgattacgc gaatggatgc catatgcaga taatctttgg agaaaaatgc gtgtcattac
     3541 tacgactatt ttttgccgcc gtcttaatgc tatggtgcgc tcaaaccgct gcttatagcg
     3601 ggcagtgtca taccactcag gggaatccgt atattggcgt caattttggc gttaaaaccc
     3661 tggaggaaga agaaaatacg actggggtag taaaagacaa attttatcag tggaacgaat
     3721 cgaatgatta ttatgtttcc tgtgattgcg ataaagacaa tgtcagaagt ggccgatggg
     3781 cattcgccgc ggattcaccg ttagtctatt taggcgacaa ctggtacaaa attaatgact
     3841 atcttgccgc caaagtttta ttgcaggtta aaggcagttc tcctacagcg gttcctttcg
     3901 aaaacgtggg gactggggca gatacccggt ggcatatttg tgaccccggc ggtcaacgtt
     3961 taggcggcca gggagctagc ggtaatagcg gtagcttttc cctgaaaata ttgcagccgt
     4021 tcgttggttc ggtcgtcatt cctcctatgg cgctggcgcg attatttgaa tgctacaaca
     4081 tacccgcagg tgattcctgc acgactacag gcacaccggt tttagtgtat tacctgtctg
     4141 gtactatcaa ttcacttggc tcatgttccg tcaatgccgg agaaacaatc gaggtcgatc
     4201 tgggcgacgt atttgcggct aactttcgtg ttgtagggca taagcctctt ggggccagaa
     4261 cggcagaact tgcaattcca gtcaggtgta acacgggaaa cgcggggtta gttaacgtca
     4321 acctgagtct gacggcaacc acagacccca gctatcccca ggcgattaag acgtcacgtc
     4381 ctggcgtggg cgtggtggtg accgatagcc agaacaacat tatttcccct gctggtggaa
     4441 cattaccgct ctctattcct gatgatgcag acagtatcgc gtgaatgaat gtctatccag
     4501 tcagcacgac aggtgtacca ccagaaaccg ggcgatttga agccacggca acggtgagaa
     4561 taaattttga ttaacgcgtg aacgtatgaa gaaaaaaaac gatatttcag tgcgttatct
     4621 tgttctttag cattcttaac atccatgtcg ggatggctgg gcctgaacaa gttagtatgc
     4681 atatttatgg gaatgtggtc gatcagggct gtgatgtcgc caccaaaagt gcattacaaa
     4741 atattcatat tggtgatttt aatatcagtg attttcaggc cgcgaatacc gtaagcactg
     4801 ctgctgattt gaatatcgat atcaccggtt gtgccgctgg tattactggc gcggacgtcc
     4861 tttttagcgg cgaggctgac acccttgcgc cgacactgct caaactaact gacacaggcg
     4921 gaagcggtgg tatggcaacg gggattgccg tgcaaattct tgatgcgcaa agtcagcaag
     4981 aaatcccgct caatcaggtc cagcctctta cgcccttaaa agccggggat aacacactca
     5041 aatatcaact tcgttataag tccacaaagg cgggagcaac gggcggtaat gcgacggcgg
     5101 ttctctattt tgatctggtt taccagtgaa ggagtgatgt tgaaacggat aatctggatt
     5161 ctgttcttac tgggattaac gggggggtgt gagctatttg cccatgatgg cacggtcaac
     5221 attagcggat cgtttcgccg taatacatgc gtgctggcac aggatagcaa gcaaatcaat
     5281 gtgcagttag gcgatgtctc actgacacgt ttttctcatg ggaattatgg cccggagaaa
     5341 tctttcatca ttaatttgca ggattgcgga acagatgtca gcacggttga cgtcaccttt
     5401 tcaggaaccc ccgacggagt gcagagcgaa atgttgtcca ttgaaagtgg tacggatgca
     5461 gccagcgggc tggcaattgc gattctggat gatgcgaaga tattgattcc gcttaaccag
     5521 gcgagtaaag actacagcct acatagcggt aaggtgccat taacttttta tgcgcaattg
     5581 cgacctgtca atagcgatgt gcagtcgggt aaagtgaatg ccagcgcgac atttgtactt
     5641 cattatgact aatacgtgga acagattggc gctcttgatt ttcgccgttt tatcgttgct
     5701 ggtggcgggt gaattgcagg caggagtggt ggtcggcgga acgcgattta tctttccggc
     5761 agacagagaa tcgatatcta ttttactgac taataccagt caggaatcct ggcttattaa
     5821 cagtaaaatc aaccgcccaa cgcgttgggc cgggggtgaa gcgtcgacag tgccagcacc
     5881 attactggcc gctccgccac ttattctcct gaagcccggt acgacaggca cgttgcgctt
     5941 gctgagaacg gaaagcgaca tcttgcctgt ggatcgcgaa acgctatttg agttaagcat
     6001 tgccagcgtg ccatccggca aagttgaaaa tcagagcgta aaagtggcga tgcgctcggt
     6061 atttaaactg ttctggcgac ccgaaggttg ccgggcgacc cgctggaagc ttatcaacaa
     6121 ttacgctgga cacggaagtc gcagggtgta caactcacta acccaacgcc ttattacatt
     6181 aacctgattc aggtgagcgt aaatggtaaa gcgttaagta atgtgggagt tgtgccgcct
     6241 aaaagccagc gtcagacaag ctggtgtcag gcaatagcac cgtgtcatgt tgcctggcgg
     6301 gcgataaatg attacggcgg gttgagtgca aagaaggagc aaaatctgcc ctgaaacagg
     6361 ttcggaaaac gtttgcgttt tttttgccgc aggtcaattc ccttttggtc cgaactcgca
     6421 cataatacgc ccccggtttg cacaccggga atccaggaga gttcatgtac taccccttcg
     6481 ttcgtaaagc ccttttccag ctcgatccag agcgcgctca tgagtttact tttcagcaat
     6541 tacgccgcat taccggaaca ccgtttgaag cactggtgcg gcagaaagtg cctgcaaaac
     6601 ctgttaactg catgggcctg acgtttaaaa atccgcttgg tctggcagcc ggtcttgaca
     6661 aagacgggga gtgcattgat gcgttaggcg cgatgggatt tggatcgata gagatcggta
     6721 ccgtcacgcc acgtccacag ccaggtaatg acaagccgcg tctctttcgt ctggtagatg
     6781 ccgaaggttt gatcaaccgt atgggcttta ataatcttgg cgttgataac ctcgtagaga
     6841 acgtaaaaaa agcccattat gacggcgtcc tgggtattaa catcggcaaa aataaagata
     6901 cgccagtaga gcagggcaaa gatgactatc tgatttgtat ggaaaaaatc tatgcctatg
     6961 cgggatatat cgccatcaat atttcatcgc caaatacccc aggattacgc acactgcaat
     7021 atggtgaagc gctggatgat ctcttaactg cgattaaaaa taaacaaaat gatttgcaag
     7081 cgatgcacca taaatatgtg ccgatcgcag tgaagatcgc gccggatctt tctgaagaag
     7141 aattgatcca ggttgccgat agtttagttc gccataatat tgatggcgtt attgcaacca
     7201 ataccacact cgatcgttct ctggttcagg gaatgaaaaa ttgcgatcaa accggtggct
     7261 taagtggtcg tccgcttcag ttaaaaagca ccgaaattat tcgccgcttg tcactggaat
     7321 taaacggtcg cttaccgatc atcggtgttg gcggcatcga ctcggttatc gctgcgcgtg
     7381 aaaagattgc tgcgggtgcc tcactggtgc aaatttattc tggttttatt tttaaaggtc
     7441 cgccgctgat taaagaaatc gttacccata tctaattatt tcttcgaatt cctctttata
     7501 agataatcag ggctttattt tcagccctgg ttgttttata ttcattgctg ttgcttattt
     7561 agacattttg tacttttatt gttgaggtta ttaagcgaag cgacaatgga ttgtgttgct
     7621 gcggttttat agggtagggg agaggcagat gcgaattaaa ccagacgata actggcgttg
     7681 gtattacgat gaagagcacg atcgtatgat gctcgattta gccaatggta tgctatttcg
     7741 ctcacgtttt gcgcgcaaaa tgttgactcc agatgccttt tctcccgcag gcttttgcgt
     7801 tgacgacgcc gcgctctatt tctcttttga agaaaagtgc cgcgatttta atttatctaa
     7861 agaacaaaaa gccgaactgg tgctgaatgc actggtagca attcgctatc tcaaaccgca
     7921 aatgccgaaa agctggcatt ttgtttccca tggtgaaatg tgggtgccca tgccgggcga
     7981 tgcggcctgc gtttggttga gcgacactaa cgagcaagtg aatttactgg tcgttgaatc
     8041 tggcgagaat gccgcgctat gcctgctggc acaaccctgc gttgttattg cgggtcgcgc
     8101 gatgcagttg ggcgacgcca ttaaaatcat gaacgacagg ctgaaaccgc aggttaatgt
     8161 tgacagcttc agcctcgaac aggcagtcta acgcgccaac ttaagtgcag tcttcggtac
     8221 acagctacag caaagaatgg tgccatcatc gcccattgct gattttttca gcggcgtgac
     8281 ttcgccttct aaaagctgaa cacggcaact tccacaaatg cccgcgcggc aagaataagg
     8341 gatacgaatt ccctgatttt ctaattgctc cagcaacacc tgttggttat ttccacgaaa
     8401 tgcctgtccc tgccagtcaa tatctacatt ggcgtccggt tgttgcgtga tgttggcagt
     8461 atcatcagcg gcagctgcgc cgtaaatttt tgccggagcc gttgtcagaa tttccacctc
     8521 atcgccaacg cgaatcacgc cgctattacg ggcaattaaa ttctgaccaa aatcgacatc
     8581 gccgttatcc tgggcagtgc ggaaagattg caatgttttt aatggttcgc ctgccggatg
     8641 tttttgccct ttttctgggc tgacggtggt gaaaatacag cggctacaag gtttaaccac
     8701 atcaaacacc acatcaccaa tgcgaatcac tttccaacta tcttcttccc aggctgacgc
     8761 gccggaaacc accagattgg ggcggaattg ctccattttt acactggccg gacaacgttg
     8821 ttggagatca cgtaacgagg cttcgttagc aagaaggtaa ggatagccat cagcaaatga
     8881 caggggtaca gtgttgtggc gtttcacgcg ccgggtcatt tgtggcccca cccagcgtaa
     8941 ttgcacttcg cgggagaaaa atccacttaa ccatttgttg atcgcttctg gcgcaattcg
     9001 cgcggtaaaa tgtgtgcccc aaacttcggt tggtgcgtct tgtgtggcga aatcagcaaa
     9061 acgaacatat gcgctactgc tatctggtgc ggtgagatgc aagccatcat gcacgggtga
     9121 aggagtaaat cgtaccatct ggggaaactg gcgagcggtg ataaacgtac cgtcaggttc
     9181 cgtgatcata aagatgcgat cgaaggccag accactgaca tctgccagag catgtgtaag
     9241 accaatgccg cgcatcgatt taacaggatg aataaaaagc cgggttaatg tcgccacggt
     9301 gcggtcctca aatgaaaata agccctcaac tttatgacat gacgcgctta ttagctataa
     9361 tgcgcaacaa ttttcttagc gcctgtccca ttaggctatt ttatttgtca gtttggccct
     9421 gggttgtgct cgaaatcctc acgtacttgt gtacgctccg gtttctccgc gctgtccatg
     9481 tccagactga ctgcaacaat tacgcctact gcggtaggct cttagagtaa aagtgacgat
     9541 atgaattctc tgtttgccag tacggcccgt gggctggaag agctgttaaa aactgaactg
     9601 gaaaacctgg gggccgttga atgccaggtg gttcagggtg gggtccattt caagggcgac
     9661 acacggcttg tttaccagag cctgatgtgg agccgcctgg cctcgcgtat tatgttgccg
     9721 ctgggcgagt gtaaggttta cagcgattta gacctctatc tcggtgttca ggcgatcaac
     9781 tggacagaga tgtttaatcc tggcgcgacc ttcgctgtcc acttcagtgg tttgaatgac
     9841 actatccgca acagtcagta cggcgcgatg aaagtgaaag acgcgatcgt cgatgctttc
     9901 acgcggaaaa atctgccgcg tccaaatgtt gatcgcgatg cgccggatat ccgcgttaac
     9961 gtctggctgc ataaagaaac cgccagtatc gcccttgatc tcagtggtga tggtttacat
    10021 ctgcgtggct atcgcgatcg tgctggtatt gcgccgatca aagaaaccct ggcagccgct
    10081 attgtgatgc gatccggctg gcagccagga acaccgttgc tcgatccgat gtgtggttcc
    10141 ggtacgttgc tgattgaagc ggcgatgctg gcgaccgatc gcgcaccagg cttgcaccgt
    10201 gggcgttggg gctttagcgg ctgggcgcag catgatgaag ctatctggca ggaagtgaaa
    10261 gcggaagcgc aaactcgcgc ccgtaaaggc ctggctgagt atagctctca tttttacggt
    10321 tcggacagcg acgctcgggt gattcaacgt gcacgcacta acgcccgtct tgcggggatt
    10381 ggtgaactga tcacctttga ggtgaaagat gtcgcgcaac tgaccaatcc gctgccgaaa
    10441 gagccgtacg gcacagtgtt aagcaacccg ccatacgggg aacgtctgga cagcgaaccg
    10501 gcgctgattg cgctgcatag cctgctgggt cggatcatga aaaaccagtt tggtggctgg
    10561 aatctctctt tgtttagtgc ctcgccggat ctgctaagct gcttgcagct gcgtgcagac
    10621 aaacagtaca aggcgaaaaa cggcccgctg gactgcgtac agaaaaatta ccacgttgcc
    10681 gaaagcactc cagacagcaa accggcgatg gtagcggaag actacgccaa ccgcctgcgt
    10741 aagaacctca aaaaattcga gaagtgggct cgtcaggaag ggattgaatg ttaccgcctg
    10801 tatgacgccg atctgccgga atataacgtt gccgttgacc gttatgccga ctgggtggtg
    10861 gtgcaggagt atgcgccgcc aaaaactatt gatgcgcaca aagcgcgtca gcgtctgttc
    10921 gatattatcg ctgcaaccat ttcggtactg gggattgcgc caaacaaact ggtgctgaaa
    10981 acccgtgaac gtcagaaggg caaaaatcag taccagaaac tgggcgagaa gggcgagttt
    11041 ctggaagtta ccgaatataa cgctcacttg tgggtgaacc tgacggatta tctcgatact
    11101 ggtctgttcc tcgatcaccg tatcgcccgt cgtatgctcg gccagatgag caaaggcaaa
    11161 gatttcctca acctgttctc ttataccggc agcgccaccg tgcatgcggg attaggcggt
    11221 gcacgcagca ccaccaccgt ggatatgtcg cgtacttatc tggagtgggc agaacgcaac
    11281 ctgcgtctga atggcctaac cgggcgtgcg catcgcctga ttcaggccga ttgcctggcg
    11341 tggctgcgtg aggcaaatga acagttcgat ctgatcttta tcgatccgcc aaccttctct
    11401 aactcaaaac gaatggaaga tgcgtttgat gttcagcgcg atcatctggc gctgatgaaa
    11461 gatttgaaac gtctgctgcg tgcaggtggg acgatcatgt tctcgaacaa caaacgtggc
    11521 ttccgtatgg atctcgacgg cctggcaaaa ctgggactga aagcacaaga aattacgcaa
    11581 aaaacgctct cccaggattt cgcccgtaac cgccagatcc acaactgctg gctgattacc
    11641 gcagcctgaa aggaatagta atgtcattaa tcagtatgca tggcgcatgg ctgtcgttca
    11701 gcgacgcgcc gcttctcgat aacgcagaac tgcatatcga agataacgaa cgtgtttgtc
    11761 tggtgggccg caacggcgca ggcaaatcga cgttaatgaa aatcctcaac cgtgaacaag
    11821 ggctggatga cggtcgcatt atttacgagc aggatttgat tgtagcgcgt ctgcaacagg
    11881 atccgccgcg taacgttgag ggtagtgttt atgatttcgt tgccgaaggc attgaagaac
    11941 aagcggaata cctgaaacgc tatcacgata tttcgcgcct ggtgatgaac gacccgagcg
    12001 agaaaaatct caacgaactg gcgaaggttc aggaacagct ggatcaccac aacctgtggc
    12061 agctggaaaa ccgcatcaac gaagtgctgg cgcaactggg gttagatcct aacgttgcgc
    12121 tgtcgtcgct ttccggcggc tggttgcgta aagcggcatt aggacgcgcg ctggtgagca
    12181 atccgcgcgt attgctgctg gacgaaccga ctaaccacct ggatatcgaa accatcgact
    12241 ggctggaagg gttcctcaaa accttcaatg ggacgattat tttcatctcc cacgaccgtt
    12301 cgtttatccg caatatggcg acgcgcattg ttgatctcga tcgcggcaag ctggtgacct
    12361 atccgggcaa ttacgatcag tacctgctgg aaaaagaaga agccctgcgc gtggaagaac
    12421 tacaaaatgc cgagtttgat cgcaaactgg cgcaggaaga agtgtggatt cgccagggga
    12481 tcaaagcacg ccgtacccgt aatgaaggcc gcgtacgcgc cctgaaagcg atgcgtcgcg
    12541 agcgtggcga acgtcgcgaa gtgatgggta ccgcaaagat gcaggtggaa gaggccagcc
    12601 gctccggtaa gatcgttttc gaaatggaag acgtttgcta ccaggttgac ggaaagcaac
    12661 tggtgaaaga tttttctgcc caggttctac gtggcgacaa aattgccctg attgggccga
    12721 atggctgcgg caaaaccacg ctgctgaaac tgatgctcgg tcagcttcaa gcggacagcg
    12781 ggcgtattca cgttggcacc aaactggaag tggcttattt cgatcagcac cgcgcggaac
    12841 tggatcccga taaaacggtg atggataacc ttgccgaagg taagcaagag gtgatggtta
    12901 acggcaagcc acgccacgtt ttgggctatt tgcaggactt cctgttccat ccgaaacggg
    12961 cgatgacgcc agtacgtgcg ctttctggcg gtgagcggaa ccgcttgctg ctggcgcgtt
    13021 tgttcctcaa accaagcaac ttattgattc ttgacgaacc gaccaacgat cttgatgtcg
    13081 aaacgctgga actgctggaa gaactgatcg acagctacca gggcacggta ttgctggtaa
    13141 gccacgatcg tcagtttgtc gataacaccg ttacagaatg ctggatcttc gaaggcggcg
    13201 gtaaaattgg tcgttatgtc ggcggttatc atgatgcccg tggtcagcaa gagcagtatg
    13261 tggcgctcaa acagcctgcg gtgaaaaaaa acgaagaacc cgccgcgcca aaagcagaaa
    13321 ctgtaaaacg cagcagtagc aaactaagct ataaattgca gcgcgaactg gagcagctac
    13381 cgcaattgct cgaagatctg gaggcgaagc tggaagccct acagacgcag gtggcggatg
    13441 cttccttctt cagtcagccg catgagcaga cacaaaaagt gctcgccgat atggctgctg
    13501 cagagcagga gctggagcaa gcctttgaac gctgggagta tcttgaagcg ttaaaaaatg
    13561 gtggctgatc gcaaaatagt cgatttaaac agagacagct gacaacggta aaattgcctg
    13621 atgaactggg tttatcaggc atgaaaacaa acactttgtt agtcatctgt aaaatagcgc
    13681 atcattaagg agtaccaatg tgcgaacatc atcatgccgc gaagcacatc ctgtgctcgc
    13741 agtgtgacat gctggtggcg ttaccgcgcc ttgagcatgg tcagaaagcg gcatgtcccc
    13801 ggtgtggcac aacgttaacc gtggcgtggg atgccccccg gcagcgtccg accgcctatg
    13861 cgttggctgc actgttcatg ctgttgctgt ccaacttgtt tccttttgtg aatatgaacg
    13921 ttgcaggagt taccagtgaa attacattac tggaaattcc cggcgtgctt ttttctgagg
    13981 actacgccag cctcggcacc tttttcctgt tgtttgtgca actggttccc gcgttttgtc
    14041 tgataaccat tctgttactg gtgaatcgcg cggaattacc ggtccgttta aaagagcaac
    14101 tggcacgggt gctttttcaa ctcaaaacct ggggaatggc ggagattttc ctcgcgggtg
    14161 tgctggtcag tttcgttaaa ctgatggctt acggcagcat tggcgtaggc agcagctttc
    14221 tcccctggtg tttattttgt gtcctgcaac tgcgcgcttt tcagtgcgtt gatcgtcgct
    14281 ggttatggga cgatatcgcc ccgatgccag aactgcgcca gccgctaaaa ccaggcgtca
    14341 cggggatacg tcaggggctg cgttcgtgct cctgttgtac ggcaatcctt cctgctgatg
    14401 aacccgtgtg cccgcgctgt ggtactaaag ggtacgttcg acgtagaaac agcctgcaat
    14461 ggacactcgc gctgcttgtt acgtccatca tgctgtatct tccggctaat attttgccca
    14521 tcatggtgac ggatttatta ggctcgaaga tgccatcgac gattctcgct ggggtcatcc
    14581 tgttatggag cgaaggctct tatcccgtcg ctgcggtgat ctttctggcc agtattatgg
    14641 tgccaacgtt aaagatgatc gccatcgcgt ggctgtgttg ggatgccaaa gggcatggca
    14701 agcgcgacag tgaaagaatg catttgattt atgaagttgt tgagtttgta ggccgctggt
    14761 cgatgattga cgttttcgtt atcgcggtgc tctcggcgct ggtgcgtatg ggaggtttaa
    14821 tgagtattta tccggcaatg ggtgcattaa tgtttgcttt agtcgtcata atgacaatgt
    14881 tttctgctat gacgtttgac ccgcgtttgt cgtgggatcg tcaacctgaa tcagagcatg
    14941 aggagtcctg acagtatgga atctaataat ggggaagcca aaatccagaa agtgaagaac
    15001 tggtctcccg tgtggatatt tcctatcgtc acggcgctca ttggggcctg ggttcttttt
    15061 tatcattaca gccatcaggg accggaagtg accctgatca ccgcgaatgc ggaaggaatt
    15121 gaaggtggca aaaccaccat taaaagccgt agcgttgacg tcggcgtggt tgaaagcgcc
    15181 acactggctg atgatttgac acacgttgaa atcaaagcgc ggctgaattc cggtatggaa
    15241 aaattgctgc ataaagatac cgtcttttgg gtggtgaaac cgcagattgg tcgagaaggg
    15301 attagcggtc tgggaaccct gctgtctggg gtttatatcg aactgcagcc aggcgcgaaa
    15361 ggcagcaaaa tggataaata cgatttgctg gactcgccac cgttggcccc gcctgatgcg
    15421 aaaggtatcc gtgtggttct cgatagcaaa aaagccgggc agctctcgcc aggagatccg
    15481 gtgctgttcc gtggctatcg ggtaggttcg gttgaaacca gcaccttcga tacgcaaaaa
    15541 cgcaatatca gttatcaact gttcatcaat gcaccttatg accgactggt gaccagcaat
    15601 gttcgcttct ggaaagatag tggcattgcg gttgatctga cgtcagcggg aatgcgtgtg
    15661 gagatgggct cattgacaac gctgctgagt ggcggtgtca gctttgatgt gccggaaggt
    15721 ctggatttag ggcagccagt ggcaccgaaa acagctttcg ttttgtatga tgatcagaag
    15781 agcattcagg attcgttgta caccgatcac attgattatc tgatgttctt taaagattcg
    15841 gtacgcggtc tgcaaccggg agctccggta gaattccggg gtattcgcct cggtaccgta
    15901 agcaaagtgc cattctttgc gccgaatatg cgtcagacat ttaacgatga ttaccgtatt
    15961 ccggtgctga ttcgtatcga gccagagcgg ctgaaaatgc agcttggcga aaatgcggat
    16021 gttgttgagc accttggcga attgttgaaa cgtggtttac gcggatcgct gaaaaccgga
    16081 aacctggtca ctggcgcact gtatgttgat ctcgatttct atccaaatac gcctgcaata
    16141 accggtattc gtgaatttaa tggttatcag attatcccga ccgttagcgg cggcctggcg
    16201 caaatccagc aacgactgat ggaagcgttg gataagatca acaaactgcc attgaatccg
    16261 atgattgaac aggcaaccag tacgctttct gaaagtcagc gcacaatgaa aaacctgcaa
    16321 acgacgctgg atagcatgaa caagatcctc gccagccagt cgatgcagca gttgccgacg
    16381 gatatgcagt caacgttgcg tgaattgaat cgcagcatgc agggcttcca gcccggctcc
    16441 gcagcctaca acaagatggt ggcggatatg cagcgccttg atcaggtgtt gcgagaactg
    16501 caaccggtgc tgaaaacgct caatgagaag agtaacgcgc tggtatttga agcgaaggac
    16561 aaaaaagatc cagagccgaa gagggcgaaa caatgaaaaa gtggctagtg acgattgcag
    16621 cactgtggct ggccggatgc agctccggcg aaattaataa aaactattac cagttacctg
    16681 tggtgcagag cggtacacaa agtaccgcca gccagggcaa tcgcctgtta tgggtagagc
    16741 aggtcgctgt acctgactat ctggcgggga atggtgtggt ttatcaaacc agtgatgtga
    16801 agtatgtgat tgccaacaac aacttgtggg ccagcccgtt ggatcaacag ttgcgcaaca
    16861 ccctggtagc caacctgagc acgcaactgc ccggctgggt ggttgcctcc cagcctctgg
    16921 gaagcgccca ggacacgctc aatgttaccg taacggagtt taacggtcgc tatgatggca
    16981 aggtcattgt cagtggtgag tggctgttga accaccaggg acaactgatc aaacgtccgt
    17041 tccgtctgga aggagtgcaa actcaggatg gttacgatga gatggttaaa gtgctggccg
    17101 gtgtctggag tcaggaagcc gcttctattg cacaagagat aaagcgtcta ccttaattat
    17161 aaagatttgt aaatataacc gtctccggta tgttgcctga ggcggttttt ttgtttctaa
    17221 cgtgcggaaa aatttgttcc tcttcacatt ttttgtacaa ccgacatgcc cgtgtagctc
    17281 acaaatatga cagtggcgtg aattttgcgc attgacggca gttatgattc gcggtattgc
    17341 ttaactgtga ttgcacattt agtaatcact gttttctttt ccaccagaaa ccagtatgag
    17401 ggaaacgagg catgaagaga caaaaacgag atcgcctgga acgggcacat caacgtggtt
    17461 atcaggccgg catcgccgga cgctcaaaag aaatgtgtcc ctatcagacg ctgaatcaaa
    17521 ggtcacaatg gctgggaggc tggcgagaag ccatggcgga cagggtagta atggcctgat
    17581 tctgtctctt taaaaagaaa cctccgcatt gcggaggttt cgccttttga tactctgtct
    17641 gattataatc agaaggcaga cgtatcctgg aacagaccga ctttcaggtc gctggcggta
    17701 tagatcagac gaccatcaac cagcacttcg ccatccgcca ggcccataat cagacgacgg
    17761 ttaacaatgc gtttaaagtg aatacggtag gtcacttttt tcgctgtcgg cagtacctga
    17821 ccagtgaatt tcacttcgcc aacgcccagc gcgcggcctt taccttcgcc gcccagccag
    17881 ccgaggtaga accctaccag ctgccacatt gcgtccaggc ccaggcatcc cggcataacc
    17941 ggatcgccaa taaagtggca tccgaagaac cacagatccg gattgatatc cagttctgct
    18001 tcaacatacc ctttgtcgaa gttaccaccc gtttcggtca ttttgaccac acggtccatc
    18061 atcagcatgt tcggtgctgg caattgcggg cctttagcgc caaacagttc accgcgacca
    18121 gaggcaagaa ggtcttcttt tgtataggat tcgcgtttat ctaccatgtt ctctgtaagc
    18181 cttattttat tgaagcacgc aggatagcta acacgtgtac gctgaacaag tccgatcagt
    18241 tcggaataaa ccagttcagc caacgtaatg gccatggaaa acggtgacgt ccttcctgtt
    18301 gcgatgcttg cgcgatacgt tcctggatgg tttgcatcag cgtcgtttgg ccttcgccat
    18361 cccacaccag atttaataat aacggcagtg cgtcagtcac atcgtctact gcccagatgg
    18421 tgaatttgcc ttcttctacc gctttcacca gttcactgtg aagacttaaa tggcgaacgt
    18481 tagctgtggg gataatgaca ccttgtttcc cggttaactc acgttgctgg caaatagcaa
    18541 agaagccttc gattttctca tttaaaccac cgaccggctg ggcgcgaccg aactgatcga
    18601 ctgaacctgt gatagcgata ctctgattca ccggcacatc ggcgagggcg cttatcaggg
    18661 cgcagagttc agccatcgag gcactatctc catcaacttc actgtatgac tgctcaaatg
    18721 tcagcgatgc tgagaagggg atctgttgct caagctgtag ttccgacatc aggaacgctt
    18781 gcatgatcat catccctttc gcatggatat tgccgccaag ctccgctttg cgttcgatgt
    18841 cggtgaattc accatcgcca atatgcacaa cgcagctaat gcgagaaggt tcgccaaaag
    18901 cgcgtggatg acccggaaat tcaatgaccg aaagggcgtt aatttgcccg atgcgttcgc
    18961 cttcggtttc aatcaggatt tgctcctgaa ggatctcatc ctgcatacgt tcagcgagga
    19021 aaccttcgcg ccattcacgc tgctgcagca ttaagtttag ctgctcgccg gagaaggtgt
    19081 cgccatcaca cagggaggcg acctctttac actggcggag gatccactgc gggctaagcg
    19141 gaagtgtttc ttgttcaccg gtgtagcgtg ctgcttcgcg gataagtacc ggccaggcat
    19201 ccgctcccgg ggcaggtaag tgattatgtc tggcggtaaa tgtcacccag cgacaccact
    19261 gggttactga ctccgcatcg acaatctgca gagtatcttc aaattcgcta taaatagcct
    19321 gctctgaaag ctctggctcc atctcctgga aatcagccaa tgattcgcgt tcgcctacca
    19381 gaatgacttt cagcttcaat ggcatcgaag gcacagagac ggggagaggg cgcgactcat
    19441 caaacgcaac ccagtcaaaa cgctcgcggt taacgatatt tttcagccgc atccacagca
    19501 gaggttgcgc cagcagtgta cgcaaagaga tgatgagaat accgccattt gcctgatgca
    19561 ccagaccagg ctgcagggta atgtcgccat taaactggcg caggcagcca aagagttgct
    19621 ccgcttctac ccagtcggca gcgacaactt gcgttaaagt cgcaaaatta tcatctgcac
    19681 tcactgcgtg acgtaagcgg atggagtggc cggaaacctc atagtgaccg cccaccagtt
    19741 ggcctgcatc gctttgtagc gtacgcgcgg cattggcaat aagattcaga tactcagact
    19801 cttccggggc cttcgccagc ataaaagagg aggatgctcg cgtatgcagc aactgctcca
    19861 gcgcaaattg cagccgcggt tgagtatcac tgaataaagg atcgttttcg tcaatcaaat
    19921 gtggctgagc aaatatttcc tgatagctat cggtatcagg aaccaggtca cgccatgcaa
    19981 gtttcgtaat ggtcaaagtt gatgtttttt agtctgttgt caaagccgcg attataccgt
    20041 aaccggcact acagcacacg tagaaagcac cgacaatact cctggcatgg gcgttaaagc
    20101 tcacaggatg gagattcttt tcttcactgg cctaaaaagc tgatattctg taaagagtta
    20161 cacggtaaca ttgagatcgc tatgaaatat caacaacttg aaaatcttga aagcggttgg
    20221 aaatggaagt atctggtgaa gaagcatcgc gaaggggagt taatcacccg ttacatagaa
    20281 gccagtgccg cccaggaagc cgtcgatgtg ttgctctcgc tggaaaatga acccgtgctg
    20341 gtaaatggct ggattgacaa gcatatgaat ccggaactgg tcaatcgcat gaagcaaacc
    20401 attcgggcaa gacgaaagcg ccattttaat gcggaacacc agcatacgcg caaaaaatcg
    20461 atcgatctgg aatttattgt ctggcaacgt ctggctggtc ttgcgcagcg tcgcggtaaa
    20521 acgctttctg aaacgattgt tcagctgatt gaagatgcgg aaaacaaaga gaaatacgcg
    20581 aataaaatgt cttctctgaa gcaggatctg caggcattgc tgggtaagga ataactgacg
    20641 aaagtcagtt caatttacta aaggcaaaaa aaccccgcag cagcggggtt tttctaccag
    20701 acgagaactt aagcttgcgg ctgagttaca acgtctttga tacctttaac ttcgatctct
    20761 acgcgacgat ccggagccag gcagtcgatc agtgcagcac gctgtttcac gttgtcacag
    20821 gtgttgccag taaccgggtt ggattcgccc ataccacgtg cggagatctt gtctgccggg
    20881 atacctttgg agatcaggta atcaacaaca gactgagcac ggcgctcgga cagaccctgg
    20941 ttgtaagcgt cagaaccgat gcggtcggtg taacccagaa caactacgga accgtctttc
    21001 ggatccaggt tgctcagctg gctgtacagc tgatccagag cagcctgacc ttccggtttc
    21061 agggttgctt tgttgaagtt gaacagaacg tcagacttca gagtgaagtg cttggtctgt
    21121 acttccggtg ccggagctgg agccggagca actactggag ctgcttcgcc ctgaccgaaa
    21181 cggtaggaaa cacccaggct cagcatgccg ttgtccggac gagtgccgat ggtgtgtgcg
    21241 tcaccgatgt tgttggtcca ctggtattcc agacgggtag cgatttcagg agtgatcgcg
    21301 tactcaacac cgccagcgaa gaccggagaa acgccggtgt cgtggttttt accataaacg
    21361 ttggatttag tgtctgcacg ccataccata ccacccagac gagtgtagat gtccaggtcg
    21421 tcagtgattg ggtaacccag tttagcggtc agttgaacgc cctgagcttt gtatgcaccg
    21481 ttttcaacgc tgcctttgta cggcatacga cctaaccagt cgtaacccat ttcaaagcca
    21541 acatacgggt taacctggta accaccaaaa gcaccagcgc ccagttggtt ttcatgggtc
    21601 gggccattgt tgttgatgaa accagtgtca tggtactggg accagcccag tttagcacca
    21661 gtgtaccagg tgttatcttt cggagcggcc tgcgctacgg tagcgaaacc agccagtgcc
    21721 actgcaatcg cgatagctgt ctttttcatt ttttgcgcct cgttatcatc caaaatacgc
    21781 catgaatatc tccaacgaga taacacggtt aaatccttca ccgggggatc tgctcaatat
    21841 taactctacc gatatcttcg gcttatgccg agcacccctg gcgatgtaaa gtctacaacg
    21901 tagttgaaaa cttacaagtg tgaactccgt caggcatatg aaaaaaaagt cttgtataag
    21961 gtatgtttaa tcttttttgt cagcgacaat ttacagaaga gaatcgcgga aaccgcttca
    22021 gacaagcctc cgcaaggaaa attagtcacg actgaaagca ttggctgggc gacaaaaaaa
    22081 gttccaggat taatcctaaa tttacttaat gatacaaatt agagtgaatt tttagcccgg
    22141 aaagttgtct cgtggcgtga gaggatgcgc ttaccggacg cataataaac cccatagcgt
    22201 taccttcatt tgccgcatca acaagttcag catgctcttc ttcagtcaaa tcatctgcca
    22261 accaaccgat caccacactg taattgcccg tgcgtaaagc gcgaaccatt gactccacag
    22321 tgtggcaagg ggagagctgg ctaatctgca ttactttcgt taagggtagc ccagatgcct
    22381 gaacccattc ccgactcagt ttttgttgcg gtgttaacca gagttgccag cgcgattgct
    22441 gaccgagttg ctgtaacaat ggcaacagta gaagttgcgt catcatgggc tgatcttcgc
    22501 gatagacaac ttcactgata agcccggctg tagtgttttc cgtagagaca cgcgcaattt
    22561 tacttgctgc ggatgagaac gacgaagaac gatgtgcata gcctgaagtg tacataatca
    22621 atccagcccc tgtgagttac tgtatggatg tacagtacat ccagtgacaa caaagatcaa
    22681 ccctattttc ggaaagagcc tcgcaaattt tgtcgttggt gacgggaaaa cataaattaa
    22741 tcttgccgct taagaataag ttgcctattt tcgtagttaa cggatccgtt aatgtgaatc
    22801 attcttttat gttatgattt taaaaggaat tttatgaaaa gcctctccta taagcggatc
    22861 tataaatcac aagaatacct ggcaacgttg ggcacaattg aataccgatc attgtttggc
    22921 agttacagcc tgaccgttga cgacacggtg tttgcgatgg tttctgatgg tgagttgtat
    22981 cttcgggctt gtgagcaaag tgcacagtac tgtgtaaaat atccgcctgt ctggctgaca
    23041 tataaaaagt gtggccgatc cgttaccctc aattactatc gggttgatga aagtctatgg
    23101 cgaaatcaac tgaagctggt gcgtctgtcg aaatattctc tcgatgcagc gctgaaagag
    23161 aaaagcacgc gcaatacccg ggaaagactg aaagatttgc ccaatatgtc ttttcatctg
    23221 gaagcgattc tcggggaggt ggggattaag gatgtacggg cgttacgtat acttggggca
    23281 aaaatgtgtt ggttgcgact gcggcagcaa aacagtctgg tgacagaaaa gattctgttt
    23341 atgcttgaag gtgccattat cggcattcat gaagctgcgc tcccggtggc acgccgccag
    23401 gagcttgcag aatgggctga ctctcttacg ccgaaacagg agtttcctgc ggaacttgag
    23461 taatctggcg ttgcagacga ccaatctcag gcagtaatgc aatcaatagt ccaacttgtt
    23521 gtacgaccag aggttctttg ctgtctgccc gtggttcaag ttgctgcatc cgctgtttca
    23581 ggctagctaa tgcctcattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
    23641 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
    23701 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaaccgaa
    23761 acgcggcttc gcgaatttgc ggggtaacgt tcggctcgct ggacatattt gataccaccg
    23821 acgccagctc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
    23881 tatcacgccc ctgatggtat tgctccagta tggcatcgag ataccgacag ttggcctctg
    23941 tggcgcgttc gagcatgcgc ggcagattgc gaaactgcca gtcaggccag atgtagctca
    24001 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
    24061 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
    24121 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
    24181 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatg gcgataccta
    24241 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
    24301 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atctgaatga
    24361 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
    24421 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caacccgtgc gggctgtcat
    24481 cagcgagctc attttcgtca ttattatggg gtagtgcctg ggcctgttct gattcaattg
    24541 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
    24601 aatcggcggg tgcgccgtta tcgcgcatcc gctccagcgc agcatcaata tgcgtaaaag
    24661 cgcgctcaaa atgcggatca tgttgataag gctgacgcaa caaaatacag cgtgacagtt
    24721 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
    24781 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
    24841 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
    24901 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agcagaccgt
    24961 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
    25021 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
    25081 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
    25141 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
    25201 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
    25261 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
    25321 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
    25381 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
    25441 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
    25501 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
    25561 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
    25621 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
    25681 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
    25741 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
    25801 agaaaatatt caataccgta ccgccagtat tcagcagcac atttttgcca gccgggttca
    25861 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
    25921 agcaggatcg tgtcagcggt aaggtaaaaa tcagcgcaat actgaccaga gtcgccaaca
    25981 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
    26041 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
    26101 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
    26161 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
    26221 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
    26281 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
    26341 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggta ccgaatgggg cgagacgcag
    26401 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
    26461 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
    26521 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
    26581 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
    26641 tgtcaggcct ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
    26701 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
    26761 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggcaggtgca
    26821 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
    26881 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
    26941 gagcgtattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgctg
    27001 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
    27061 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
    27121 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
    27181 gaaggtaact tctgggatga tgaaaaatta cagcgtcgtc ttgcctcacg cctcgatcgt
    27241 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
    27301 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
    27361 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
    27421 attgtgattc tggagaaagg tcgctttatc agcccgtgga agcatattct ggttgatgaa
    27481 tttcaggata tctcgccgca gcgggcagcg ttgttagcgg cattacgcaa gcaaaacagt
    27541 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
    27601 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgaacg ctgtgattta
    27661 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
    27721 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
    27781 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
    27841 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
    27901 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
    27961 gggcaacagg cggattacgt cctcatcgtt ggcttgcagg agggaagtga tggttttccg
    28021 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
    28081 gacgctgaag aacggcggtt aatgtacgtg gcgctgaccc gggcacgtca tcgggtatgg
    28141 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
    28201 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
    28261 gacggtccgc gagataacgc tgataatcgg ggatcagaat atcgaccgcg tcgttgaaat
    28321 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
    28381 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
    28441 cccagaagaa aatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
    28501 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatta
    28561 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
    28621 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
    28681 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
    28741 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
    28801 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
    28861 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
    28921 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
    28981 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
    29041 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
    29101 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
    29161 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
    29221 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
    29281 aacgcgaaac actaactgat gcggtccatt atccagttca atgctgtccg caccgcgcag
    29341 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
    29401 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
    29461 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
    29521 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
    29581 tagctgcatg gttggcatgt cggctttgtc gtacactttg tgaaacagtc tggaggaaat
    29641 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
    29701 ggcaagcgac aaacccgatc gtcccagcta tcgcgtgatg aaatatctac tcgaccaggg
    29761 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
    29821 gtacggcacg ctggcagacg ttccggaaaa agtcgatatg gtcgatgttt tccgtaattc
    29881 agaagcggcg tggggcgtag cgcaggaagc tatcgccatt ggcgcgaaaa ccctctggat
    29941 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttgaatgt
    30001 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggcctggcca aataaaaaat
    30061 ccccggaagg caaaaacctt ccggggatct gttcagggat tagttacgca gacgcggggc
    30121 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
    30181 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
    30241 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
    30301 ctcatcaggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
    30361 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
    30421 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
    30481 gacgcgacag gcaaaccctt tcagatatag cccttccgga taggtagcga tcaccggatg
    30541 atcggccgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
    30601 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
    30661 gagaataccg ccttcattca gcagctgaat cgccagcatg ttgatatctt tatagccacg
    30721 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
    30781 gtcaaatttt tcaccgcgat cgcgataggt acgcagcaat ttaaagacat catcacggac
    30841 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
    30901 tgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
    30961 ggcgaaacca ccggtatagg agaaacagtt cagtacgcgt ttattttcaa cgtagcggcg
    31021 ggtagccagg cggctgtctc gctggtccag gtagtagccc gttttgtgtc cgtgctgtat
    31081 gtccaccagc agcttcatgc catgttcttc aatcggcagc agggcaggcg gcaactcacc
    31141 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
    31201 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
    31261 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
    31321 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
    31381 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
    31441 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
    31501 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
    31561 gctggcttta ccttccatac gggcaacggc cccggaaaag acccacggat ggcgacgaag
    31621 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
    31681 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
    31741 aaggataagg agagcagcaa tgtcgaaagt ctgcataatt gcctgggttt acgggcgagt
    31801 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
    31861 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
    31921 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
    31981 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
    32041 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg gacctttcgg
    32101 aaacagtcgg tataaatagc ggctgttacc tttttcttcg ccaaatttat tcgccatcgc
    32161 ttttaccagc atacgaatcg ccggagaagt attgaattcc agatagaaat cacgcacaaa
    32221 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
    32281 tgccagcggc tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
    32341 ctctttacct tcgaagatca gcataattat tactacctta atcagactgc cggcagtgta
    32401 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
    32461 gcggctggcg aagcccagaa tgctcagcag gctgacgaag atgttgtaca gcgaaacata
    32521 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
    32581 aaacaaaata gcgccagagg agatcaaaat gaagaccgcg ctgatcgcca gatgcagagc
    32641 aggcagctgc aggaagatat tagcaaccat accaatcagc accaccacaa tacccgccat
    32701 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
    32761 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
    32821 cagataggta ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
    32881 ggagataatc ccggtcggct tattcgccat tttataggtc aggaacatca aaccatacat
    32941 acccaccagc gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
    33001 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacttt
    33061 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
    33121 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
    33181 tagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
    33241 attaagcaaa tataacgccc tgagaatttc ggcaggcaaa agaaaaaggg gttagcattt
    33301 agctaacccc ttatcttatc tggcggaagc gcagagattc gaactctgga accctttcgg
    33361 gtcgccggtt ttcaagaccg aagaaaaaca aataaaaatc agtgtaatac gtgaaattgc
    33421 tgaaaagtta atcaaattta acaccaagaa aaatcaaggt attaggaagt tgctcatagc
    33481 tagtatacca agtctttttg ccagaaattt ttctggattg tctacttgct aaccacgagc
    33541 ggcaaatcca gtgttggtgt gatttttgtt ttacgatcgt aaatcaacac ctgattttct
    33601 gttttatgcc cgctgaaaat ttgtttgtcg cgactgctgc cttcgtaatc tgagatcccc
    33661 ttggctttta tgtcgtgaaa attgcacccg aacggaacgc caactttttg ctcggctgcg
    33721 cgtttagcct gattccacca gttattcagc gttttagcta tgactttccc gcctttggtt
    33781 gtattgatca catattcgca tgtacaggaa gatacatttc gggctaactg gatcgctgta
    33841 cgtaatcgcg gtgaccattc cttgatttgt ttggtgccgg ttttgttttg ctcaatgtag
    33901 atccctttat ccataatatc ctgccatttc aactcaagca catcaccgag tcttgccgca
    33961 cagagatagg aaatctccat tgcaatgcgt aactgtggaa ttgcttccgc atatatcgcc
    34021 gcatactgtt catcggtgat gtaaacagtg cgggctttaa gagagaattt tctgactcct
    34081 ttgcatgggt tattcttcac atacccacgc tcatatcccc acccgtatac tcgactcagg
    34141 cttgccagtt catgatttgc ccaagccctc gtttatccat gaaaattctt acctgctcag
    34201 ttttgacatt atcagcaagc acttttccga ataccatcag caacgccttc tgatgttgtc
    34261 gataatcttt ttgggttcgg ggggccagtt ctgtaaatgc aggggagtcc ataaacatgt
    34321 gccacaattt agctacggtc attatgttgt ggagttttgc tttttccagt tcataatttt
    34381 gccagacttt agctacgctg gttttccgca ctcttcctag ccctatactt cttgtacttc
    34441 cttcgggttt ccacacgtaa ctgtaaccat tcgatctaac ccgcggtggc agtacattat
    34501 cttttttatt ttttcttggt cttcccattg ttcagcgcct caaaatcggg ttcagcagaa
    34561 accagttcag atgtttttgg catcgttgtc agaccgtgtg gaatatccct gcggagaact
    34621 atgggttcgt ttttagggcc gattacaaat gggatgccgt gcagtcttaa ctggtgttgc
    34681 tgttttgtgt atcgctcgta tttcgtgatc tctttaatct ctgctggaga tagagttaat
    34741 tcgtacatgt ggtcacgtgc ctttacagca tgaccgccgc cactatagct ggtgggcggc
    34801 gatatgggtt gaacatcaaa aatcagcctg actcgggagc agtttttgcc agatggctga
    34861 aacgtatttc gcctggtaac g
//
LOCUS       DBFIFM010000025        34798 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.025, whole genome shotgun sequence.
ACCESSION   DBFIFM010000025 DBFIFM010000000
VERSION     DBFIFM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.025"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            16..92
                     /locus_tag="N7C74_001107"
     tRNA            16..92
                     /locus_tag="N7C74_001107"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50..52,aa:Met,seq:cat)
     gene            complement(166..1419)
                     /gene="amiC"
                     /locus_tag="N7C74_001108"
     CDS             complement(166..1419)
                     /gene="amiC"
                     /locus_tag="N7C74_001108"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HGU2431020.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1651..2982
                     /gene="argA"
                     /locus_tag="N7C74_001109"
     CDS             1651..2982
                     /gene="argA"
                     /locus_tag="N7C74_001109"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HGU2431021.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKSCRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3044..4870)
                     /gene="recD"
                     /locus_tag="N7C74_001110"
     CDS             complement(3044..4870)
                     /gene="recD"
                     /locus_tag="N7C74_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HGU2431022.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEESHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPTGDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4870..8412)
                     /gene="recB"
                     /locus_tag="N7C74_001111"
     CDS             complement(4870..8412)
                     /gene="recB"
                     /locus_tag="N7C74_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HGU2431023.1"
                     /translation="MSDVAETLDPLRLPFQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAKWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     RNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVSAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMMRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLNFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQARGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(8405..11293)
                     /gene="ptrA"
                     /locus_tag="N7C74_001112"
     CDS             complement(8405..11293)
                     /gene="ptrA"
                     /locus_tag="N7C74_001112"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HGU2431024.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEALKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11469..14837)
                     /gene="recC"
                     /locus_tag="N7C74_001113"
     CDS             complement(11469..14837)
                     /gene="recC"
                     /locus_tag="N7C74_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HGU2431025.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNALFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVSGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14850..15173)
                     /locus_tag="N7C74_001114"
     CDS             complement(14850..15173)
                     /locus_tag="N7C74_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HGU2431026.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLTSR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGRAGEMTRLH
                     CPNRQ"
     gene            complement(15158..15565)
                     /locus_tag="N7C74_001115"
     CDS             complement(15158..15565)
                     /locus_tag="N7C74_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HGU2431027.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCLLYAATGARVCLRLLEDNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(15562..16125)
                     /locus_tag="N7C74_001116"
     CDS             complement(15562..16125)
                     /locus_tag="N7C74_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HGU2431028.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEQVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSLVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16116..16586)
                     /locus_tag="N7C74_001117"
     CDS             complement(16116..16586)
                     /locus_tag="N7C74_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HGU2431029.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISFIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIGFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(16770..17564)
                     /gene="thyA"
                     /locus_tag="N7C74_001118"
     CDS             complement(16770..17564)
                     /gene="thyA"
                     /locus_tag="N7C74_001118"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HGU2431030.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17571..18446)
                     /gene="lgt"
                     /locus_tag="N7C74_001119"
     CDS             complement(17571..18446)
                     /gene="lgt"
                     /locus_tag="N7C74_001119"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HGU2431031.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18597..20843)
                     /gene="ptsP"
                     /locus_tag="N7C74_001120"
     CDS             complement(18597..20843)
                     /gene="ptsP"
                     /locus_tag="N7C74_001120"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGU2431032.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20856..21386)
                     /gene="rppH"
                     /locus_tag="N7C74_001121"
     CDS             complement(20856..21386)
                     /gene="rppH"
                     /locus_tag="N7C74_001121"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HGU2431033.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(21739..21885)
                     /gene="ygdT"
                     /locus_tag="N7C74_001122"
     CDS             complement(21739..21885)
                     /gene="ygdT"
                     /locus_tag="N7C74_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HGU2431034.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            22071..22760
                     /gene="mutH"
                     /locus_tag="N7C74_001123"
     CDS             22071..22760
                     /gene="mutH"
                     /locus_tag="N7C74_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HGU2431035.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22829..23542
                     /locus_tag="N7C74_001124"
     CDS             22829..23542
                     /locus_tag="N7C74_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HGU2431036.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23680..23898
                     /gene="ygdR"
                     /locus_tag="N7C74_001125"
     CDS             23680..23898
                     /gene="ygdR"
                     /locus_tag="N7C74_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HGU2431037.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            24006..25046
                     /locus_tag="N7C74_001126"
     CDS             24006..25046
                     /locus_tag="N7C74_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HGU2431038.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLAPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25078..26271)
                     /gene="lplT"
                     /locus_tag="N7C74_001127"
     CDS             complement(25078..26271)
                     /gene="lplT"
                     /locus_tag="N7C74_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HGU2431039.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHEQLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26264..28423)
                     /gene="aas"
                     /locus_tag="N7C74_001128"
     CDS             complement(26264..28423)
                     /gene="aas"
                     /locus_tag="N7C74_001128"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HGU2431040.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDPQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFIAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEVEQHDE"
     gene            29009..30040
                     /gene="galR"
                     /locus_tag="N7C74_001129"
     CDS             29009..30040
                     /gene="galR"
                     /locus_tag="N7C74_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HGU2431041.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLLKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(30047..31309)
                     /gene="lysA"
                     /locus_tag="N7C74_001130"
     CDS             complement(30047..31309)
                     /gene="lysA"
                     /locus_tag="N7C74_001130"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HGU2431042.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMFDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVEPRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31431..32366
                     /gene="lysR"
                     /locus_tag="N7C74_001131"
     CDS             31431..32366
                     /gene="lysR"
                     /locus_tag="N7C74_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HGU2431043.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLVRYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDELCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32353..33045)
                     /gene="ygeA"
                     /locus_tag="N7C74_001132"
     CDS             complement(32353..33045)
                     /gene="ygeA"
                     /locus_tag="N7C74_001132"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HGU2431044.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33174..34592)
                     /gene="araE"
                     /locus_tag="N7C74_001133"
     CDS             complement(33174..34592)
                     /gene="araE"
                     /locus_tag="N7C74_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HGU2431045.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLS
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         8533 a         9552 c         8592 g         8121 t
ORIGIN      
        1 aaagcagtac ggtgacgcgg ggtggagcag cctggtagct cgtcgggctc ataacccgaa
       61 ggtcgtcggt tcaaatccgg cccccgcaac caattattga acaccctaac gggtgttttt
      121 ttgtttctgg tctcctataa aaaagcgcca ttcagcgcct ttttatcatc cccttctcgc
      181 cagggtcgcc ccatcggcaa aatacgcttt aattcccgca agaatagact ccgcaacttc
      241 ctgctggaag gtcgccgttt tcagtttacg ctcttcctca acgttactga taaacgccgt
      301 ttcgaccaga atggagggaa tatctggtgc ctttagtacg gcaaacccgg cctgttcaac
      361 ttgattttta tgcagcttgt tgattttacc gagcttattc agcaccgctt taccaaactt
      421 aaggctgtca gcaatggtca gcgactgcac catatcgaac atggtgtggt cgacatagcg
      481 gtcaccgctt ttgctcacgc caccaatcaa gtcggaggcg ttctgggttt gtgccagata
      541 ttttgccgca gtactggttg cacctttggt tgagagcgca aacaccgagg aaccgctcgg
      601 ctggcgactg gtaaaggcgt cggcatggat agagacaaac aggtcagcac gctgtttctg
      661 agcttttgct acgcgcactt gcaacggaat gaagatgtct tcattgcgcg tcatgtacac
      721 cttcatattg ccctctttct cgatcagaga gcgcaaacgg cgagctattt gcaataccac
      781 atctttttcg cgcgttttgt atttccccac cgcaccggag tcttcgccac cgtggccagg
      841 gtcaagcata atgacaatcg gacgatcgcg ccctgcttta cctggttgtg gaccactttg
      901 cgctggcggc acctgctttt cgaggtcgcc tttgttgtaa tcctccagca gcgccagcag
      961 cgggtcctgc atatcctgtg cattggccgg atagaggtcc atcaccagac gctctttaaa
     1021 cccggcgacc ggcgcaaggg caaacagctg cggttttacg ttttgcttta attcaaaaac
     1081 catccgtacg gtttgcgggt caaattgccc gacgcgcgcc gacttgatga acgggtcgtc
     1141 tgcacgaatt tgtgccgcca tccccttgag taccgagttc aggtttacat cttcgatatc
     1201 caccaccacg cgttccgggt tactcaacgc gaactgctta tatttcagct gacgatttga
     1261 ttctaccgtc acgcgggtgt agctggacgc aggccagacg cgcaccgcca cgacctggct
     1321 gaccgcagcc agactgacct gacttacgct caatagccac atggcacccg cgccttgcag
     1381 taaacgacga cggctgattg cagtgttgga tcctgacatg cctctcccga gcaaaagaaa
     1441 tctaagctgt gtaacaagta aacgactaat ttgaccggaa actttagcga aagacgcata
     1501 atctgtcatc taataaacgg taaacattct ttttatattc acgccattac tgataaaaaa
     1561 gtcgctctcg cataaaattt acacttgcac cctgcgaaaa aacagaataa aaatacaata
     1621 aattcgaata ataatgcaaa gaggtgtgcc gtggtaaagg aacgtaaaac cgagctggtc
     1681 gagggattcc gccattcggt tccctatatc aatacccacc ggggaaaaac gtttgtcatc
     1741 atgctcggcg gtgaagccat tgagcatgag aatttctcca gtatcgttaa tgatatcggg
     1801 ttgttgcaca gcctcggcat ccgtctggtg gtggtctatg gcgcacgtcc gcagatcgac
     1861 gcaaatctgg ctgcgcatca ccacgaaccg ctgtatcaca agaatatacg tgtgaccgac
     1921 gccaaaacac tggaactggt gaagcaggct gcgggaacat tgcaactgga tattactgct
     1981 cgcctgtcga tgagtctcaa taacacgccg ctacagggcg cgcatatcaa cgtcgtcagt
     2041 ggcaatttta ttattgccca gccgctgggc gtcgatgacg gcgtggatta ctgccatagc
     2101 gggcgtatcc ggcggattga tgaagacgcg atccatcgtc aactggacag cggtgcaata
     2161 gtgctaatgg ggccggtcgc tgtttcagtc actggcgaga gctttaatct gacctcggaa
     2221 gagattgcca ctcaactggc catcaaactg aaagctgaga agatgattgg tttttgctct
     2281 tcccagggcg tcactaatga cgacggtgat attgtctccg aacttttccc taacgaagcg
     2341 caagcgcggg tagaagccca ggaagagaaa ggcgattaca actccggtac ggtgcgcttt
     2401 ttgcgtggcg cagtgaaatc ctgccgcagc ggcgtgcgtc gctgtcattt aatcagttat
     2461 caggaagatg gcgcgctgtt gcaagagttg ttctcacgcg acggtatcgg tacgcagatt
     2521 gtgatggaaa gcgccgagca gattcgtcgc gcaacaatca acgatatcgg cggtattctg
     2581 gagttgattc gcccgctgga gcagcaaggt attctggtac gccgttctcg cgagcagctg
     2641 gagatggaaa tcgacaaatt caccattatt cagcgcgata acacgactat tgcctgcgcc
     2701 gcgctctatc cgttcccgga agagaagatt ggggaaatgg cctgtgtggc agttcacccg
     2761 gattaccgca gctcatcacg gggcgaggtt ctgctggaac gcattgccgc tcaggcgaag
     2821 cagagcggct taagcaaatt gtttgtgctg accacgcgca gtattcactg gttccaggaa
     2881 cgtggattta ccccggtgga tattgattta ttgcccgaaa gcaaaaagca gttgtacaac
     2941 taccagcgta aatccaaagt gttgatggcg gatttagggt aagcgatgaa aatcgtcgga
     3001 tgcgacatgc gtaacactcg tacgtcgcat ccggcaatta cgtttattcc cgtgaactaa
     3061 acaacgccgc cagaccactg cgccgctcag tacgagtggc gattgccgca cttaatatgc
     3121 gctcatcggc atacagcgac agacggcgac gcgcgcgggt caccgcggta taaaccagct
     3181 ctcgcgttac taccggcgtg cgttggctcg gtaaaatcaa cgccgcatgg tcgaactccg
     3241 atccctgcga tttatgtacc gtcatcgccc acgtagtttc gtgctctggc aagcgactcg
     3301 gttgcacaga cttaatattg ccgtccggca tcgcaaacca gacgcgcgtc ccctgcccgc
     3361 gatccagcgc aataccgata tcgccattaa acaacccaag cgcgctgtca ttacgggcaa
     3421 tcatcaccgg tcggccttcg taccaacgag agtgcggatt acgatgaatt ttgcgcttct
     3481 gttgcataaa ctgctcaatt cgctcattca gtccagccac gccaaatggc ccttcccgca
     3541 gggcgcacaa aagttggtac tcattgaacg cctgaatgat taaatccggc tcggcacgcg
     3601 cttgcagcag atccagataa cgtccataac ccgcaagagc ttcctcaagc atcgcaatat
     3661 aatcttcgcc gctctgtaaa agccgttttt caatatcagt aaaatcctgc tgaaaaacgg
     3721 ttttcactgc cgttttatca ccacggttga tcgccgcagc taactgacca atgccagaat
     3781 cgctgccgaa acgatagctt ttttgcagca ggcagagact gtcgcgcaaa gatgccgctt
     3841 ctgtgccagt tcctgccgga acgtgagttc ccgtcaggcg gcttagctgc ccggcacgct
     3901 cggcggtaaa gccggcattg gcataagcgc agatatcgcc cagcacagcc ccagcctcaa
     3961 ccgaggccag ttgatcacga tcgccgagaa agatcactcg cgcatgatcg ggcaaggcgt
     4021 cgatcagtct cgacatcata ggcagatcga tcattgacgc ttcatctacc accagcacat
     4081 caagatgcag cgggttaccg gcatgatgac gtaaacgctg gctacccggc tgcgcaccca
     4141 gcaatcggtg caaagtactg gcatcttccg gaatacgttt cttttgttca tcggtcagcg
     4201 gtaactgtcg caaagccttg ccgagagatt cggttaagcg cgcggcagct ttacccgttg
     4261 gtgcagccag acggatacgg cagcgttcgc cgtcggccat ttgaattaac gctgccagca
     4321 acttcgctac ggtggtcgtt ttaccggtgc cagggccgcc ggaaatcacc gagatccgcc
     4381 gcgtcagcgc cactgctgcc gccacttttt gccagttaat ttcatcacca gtcggaaaga
     4441 gtttatccag ggtttgcgcc aacaaggctt catccacctc gatggcatga ttcacctcat
     4501 tgaagaagcg cgctaccgtc cgctcgttac accacatgcg attcaaataa agacgatcgc
     4561 cacagaggat catcggcgtg ggttcatctc cccggctgac cgcttgtgaa gccagcaagc
     4621 attcttccca attttgtagc tcaccgattt cactgacaca ggtcgccaac agcggatgcg
     4681 actcctcatt attttccagt cgtgaaagcg gcaaacaaac gtgcccctct ccggcatcat
     4741 gacttaacag tgccgccgcg agggtgacgg caggatgttc atctcccgcc acggtcaggg
     4801 caaactgcac gtccagcggg cgtagctgtt tgtgctccac agcttccagt aattgctttt
     4861 gcaatttcat tacgcctcct ccagagtcat accggcaaac atctcatcca tcaggtcaat
     4921 caacccggcg ttggggcggg ttgtgtaaat cccttgttgc ggatgttctt tatcaacacc
     4981 acgcaggaac agataaataa cgccgccaaa gtggcgctca tagtcgtaat cagcaatgcg
     5041 atggcgcaga taacggtgca gcgccagggt ataaagctga tattgcagat catagcggtg
     5101 tgcctgcatt gccgctgcca tagcctgttg ggtgtaagcc gaactatctt cacccaacca
     5161 gttggattta tagtcgagca ggtaataacg cccttcgtgg cggaacacca ggtcgataaa
     5221 gccttttaac atgccacgtg cctgcatgaa ctccagcggc gggcagccag cggatagcgg
     5281 gtcaaactgg cgaattagtg catcaagctg gctggcgatg agcggttcgc taatcggcag
     5341 gtaaaactcc atctccacct gtttatcgcg gtcggaaagc tgactcaggc taacacccgt
     5401 ttcattgaga ggtgcctgga ggacagccgt gatccactcg gtcaacaccg gttcccactg
     5461 cgattcaaag ccgccgagtt ccagtttttc ctgtacccag ttcgggtcaa ccggctgggt
     5521 aaaattgagg tcttcaaaca aactgtgcaa gaatgtcccc ggtgacgcac cgcgcgggaa
     5581 ctgatgcggt gttaacgtcg gttcttcaac gacgctgacc acgcctgcgg catcaacatc
     5641 cagccgtggc atcagatcct gagcgatacc gtgaccacgc tgctgcaagc cggagtagct
     5701 ggtgacacgc cagttatcgc cgggcaatcg ttgtagcgtc ctcgcattca gttctgcagt
     5761 taaagcatca ttaacctgcc agggctggtt atcaccagtt tgtgccgttt gccaggcaat
     5821 atcatcatcg cataacgctt caatacaggt gcgaagccct gccgcatctt gcggttcccc
     5881 tttttgcagc aaacgcccga gggcactttg gtggacgtcg gtgtcacctt ttttatcgcc
     5941 acgacggcgc accagcggtg caacgccgag actgcaatgc caaaccgaac gcgtcagcgc
     6001 cacgtaaagc aaacgcagat cttccgccag acgttcgacc tccgcaagat cgacgctttc
     6061 tggcgcagcg ttaagatcca gaacagcctc aaacgagtgg cgatcgtgat aaaacgcctg
     6121 atcctggacg cggaaattgg taataaatgg caaccagacc agcggatatt ccagcccttt
     6181 tgatttatgg atcgtgacaa tctgtaccag atgtttatca ctttcgaggc gcatttgttg
     6241 gctggaggca ttactgtctg gctcgaggat atgttgcgat aaccagcgta ccagcgcatg
     6301 ttcactttcc agctgcgttc cggcttcttg tagcagttcg ctgatatgca agatatcggt
     6361 aagacgccgc tcaccgcctg ccgttgccag caagttttct gcaatgttac gcgccgacat
     6421 cagcgcccgc atcatcggca taacgccacg tttgcgccag atttgccgat aaccatcgaa
     6481 ctcttcgacc accgcgtccc acgcatgttc gtcattattc agcgtttcga tatccagcgc
     6541 gttcagcccc atcattgacg ttgccagcgc actacgcagg gtgttctcac gttcgggcgt
     6601 catcaccgcc tgcaacaacc aaagcatttc ctgcgcttcc agagtttcaa aaacactgtc
     6661 gcggttcgaa aggtaaacgg aagggatttc cagcaacgtt aaggcatcgc gcacctgggc
     6721 ggcctcctgg cggctgcgca ccagcacact gatgtccgaa gcacgcaccg gacgcgcgtc
     6781 gtcgccgttc atcagcaacg cttcgccccg ctgtccggct tgtagccagt cgcgaatttg
     6841 cgcagcactt acctgcgcca tggtactttg atagtcgcca acgccgcaac tttcgccttc
     6901 catcagccac attttcatcg caggctgcgt ttcaccttta aagacaaaac gtaacgcctg
     6961 atttttcccg gctgatttca ctggaataaa cggtatttcg cgaaacatga acgcgtcatc
     7021 agtctggctg aaaagcttgt tcacactgtt caccattcca ggtgcggagc gccagttggt
     7081 gtctaaagtg tagtgggcgt gaacttcact acgcgccttc atataagtga agatatccgc
     7141 accccggaat gcatatatgg cctgcttcgg gtcgccaatc agcaacaatg cggtttccgg
     7201 ctgatggtgc cagatacggc gaaaaattcg gtactgctgg gggtcggtat cctgaaattc
     7261 atcgatcatt gccaccggga atcgcgtacg gatcgccgct gccaacacct caccgctttc
     7321 gctacgcagc gcggaatcga gccgacttaa catgtcatca aaacccaatt cgccacggcg
     7381 gcgtttttca cgcgctactg tttcgcggat ctcagccaat gcgcgggtga tcaccagatc
     7441 gcggatcgac aatggttctg caagcagttg atcgatcgcc tcaaacagtg gatgtcgcgg
     7501 ggtttccccc ccggccttcg tgcgatcttc taagaaacgc tgggagaatt tttccagcga
     7561 ctccggcaac tgataactgt ttctctcttc ttctgcccag gcgctgatct tgtcgatcca
     7621 tttagcctga ttgctacggt taaacttgcg tcgatcaata ccagaagatt cgatcagcgc
     7681 atccagttca cccactgcgt cgcgccactg ctgttttacc gtatcaatac gcgccacaat
     7741 ttgcgcgtga cgggaagcca gcgtttcatc atcgggcggc ggtgctttga taaccggcgc
     7801 ttcaccttgc agataacgat taatatcgcg cagcaacgcc tgcggccctt tccaggtttc
     7861 aaagacgacc tgggctattt cacgcggcag cgggtagcag tggcgacgcc agaaatcggc
     7921 gcaggcctgg taacgtagca gagactcatc ttcaatcagc tgctgctcaa acagcatgcc
     7981 ggattcaaag gcattcaggt tgagcatgcg ctggcaaaaa ccgtgaatag taaagactgc
     8041 cgcttcatcc atctgccgtt cggctaacaa caaccacttc gcggcttgcg ctttatcgtc
     8101 gatctcttcc agcagacgtt cgtacagtgg attgtcggtg gtttcacgca gacaggcgat
     8161 gcgcaactca tggatattgc tacggatacg accacgcaat tctgccgtgg cagcctcggt
     8221 aaaggtgacc accagcagtt cttcaacggt cagcgggcgg ggaaaggcgg cggaaccgcc
     8281 tagtccaagt aacaggcgca aatagagcgc cgcaatcgta aaggttttgc ctgtgccggc
     8341 agaggcttca atcaggcgct cgccctgaaa gggcaagcgc aaaggatcta gtgtctcggc
     8401 gacatcactc attcttttca ctcatcaggg gcattgtttg ctgcaacgcg ctgacgttct
     8461 cccacacttt ccagccttca ggatgcacat attcggcttt cccgttctgg ctgccggaaa
     8521 tctgcgacag aatagccatg ccttgcggct cgaccaccgc ctgatggaag aaatcagcaa
     8581 gtttttgcgg cgtcagcagt tttatctggg ccacgatttt atcacgcgaa tcgaagcgca
     8641 tattgccgcg atcgaaatct ttacttaact tcaatgcttc ttcgccgagc gtttgcggtg
     8701 cctgcagcat ctgggtaatt accgcctgct ggatttgcgc aaactcatct ggcttcatcg
     8761 ctcgcaattt tgcctctgcg gttgggaaaa acgccttgta acgctcccac aagaatgaag
     8821 gctgtttatc attgctttgc aaaaggaagc ccatgcccca ctgacgcccc acgctcattg
     8881 gaaacgcaaa cacggcatag cccaattgtt cttcggtacg caactgattg tagaaccacg
     8941 gctgtacgat ctgccccaac agagagctat aggctgagct tgtgtattca tcgtagccag
     9001 tcggtacaaa taccgctgcc agtgcggagt cggtgctgtt accggctttt tcaaagatga
     9061 cggattgttt tttatcgacc actacatctt tgtttcgaca ccactctgaa ccatcagcgc
     9121 ccaactgttt ttgcacatcg cgtgccagcg ttgttgcctg ggcctcggtc atgttgccga
     9181 taaccataaa ctctggtcga gcccctgatt ttaaggcgtc acgataagcc agcacctctt
     9241 tcaacgtaat ggagggcaaa attttacgcc gttcatctcg cgagaagtac ggcacttgcg
     9301 agagcatctg cgcgggcata atcgcctgct caaacgcttt acccttttct gcggaatcca
     9361 tcatctggtt ataccaggac ttcgcctgct caagctgatc ttccgtagcg gtatagctaa
     9421 agtatccctc gagcaatgcc tggaacagct gcggcagacg ctgggtgtaa ccattagcat
     9481 taaccataag gccgttgtta gcgttggtgg aaaaacttat gccaccaacc gacgcctggt
     9541 tgcttaactg atcaagcgcc agccctgcga gataatcatt gagcgcaaac atcacctgat
     9601 tgcgggcgct gtccatggct ttcggattac gcaaaatcag gctgacatca gctttgggtt
     9661 cgctggaaaa ataacggctt ggcgcataca ccacgcgcag attcgactca tcaacaatca
     9721 gctctggatg gtcatatttc ttctctgact taatcagcga gaaatcgtca ggaatatagg
     9781 ggttaagctc cggtaaggag agcgcaatat tggcagcttt ttgctgccag tcagcgaaag
     9841 tttgttcgct gattttatcg acctgatacg gcgcatcgac aaaataagcc gttttattgt
     9901 gcggctcttt cgggctgata taccagatac gcgcattctg cggcgtcatc atcgccagac
     9961 gttcttttac tgctttagca tcgtaccgat cggcaatatt gactgcatcc agcgtatgct
    10021 caacaggaac gcgaatcatg gtatctgcca gccattcgac gtaatccata tcacgggtga
    10081 ttgacggata acggaagtcg atatccagca cattcgccag ttcatcgaag tattgtttat
    10141 cgatcccttt ttcacgtaac aaattgaggt agctaaaaat agccgccaca acctgatcgc
    10201 gattcgccag acctttatcg gttaaagacg cagagatcgc taatacgccg ctgttgccgt
    10261 tgacgatagg atcggagttg gcgctaatgc cctcaactaa cccctgcttt tgcagccagt
    10321 cagacagtgt acctgggctg cgattaccaa tcagataggt aatcaattca tccgttttac
    10381 tgcggaactt cgctgagttg ttatcgatgc gaaactcaac gcgcagaact ttacgcggca
    10441 gcgctgggac gtaatgaatg ataatgccct tttgcgcgtc ggtgactacc ggcacggtga
    10501 tttccggttt tttgctctct ttgttcggca cgcgaccaaa agtgtccgcc gccatttttg
    10561 ccaactccgg cagcggttta ttactgtaaa taaccgcctt catcaaattg gcggaatagt
    10621 acttctcgtg gaaatctttc agcgcctgct gcaccggatt accaggtttg tcgcttaaag
    10681 tttcgaggtt accaccagaa aactttgaac cggggtgtgc cgggttaatg gtttctgcgc
    10741 tgacctgtgc catgcgcatc ccgtcacgtg tacgcgccat ggttaattca gcgttcactg
    10801 cattacgctc acgttcggcg tatttcttgt cgagcaaggg ttctgcaata gcatcggcca
    10861 ggcggtctac cgcaccgggc aaggcgtcgt tctcaacttc cagatagaaa gccgtgcgat
    10921 acggcgcagt gctggcattg tgactaccgc cgtgcatttt gagatattcg gccagactgt
    10981 cagcctgcgg gtactttttc gaccccatca gactcatatg ttcaaggtaa tgtgccagcc
    11041 cctggtacgc ctcgggatct tccagcgacc caacgggcac caccagcgcc gatagcgatt
    11101 taactgcctg cggatcagaa accagcaaga ccaccatacc gttatccaga cgtatagcct
    11161 gatactggcg gttatcttta tcacttttac ggatggtttc ctgaatcggc tgccatcccg
    11221 tttctgcctg acttaagggt gcccaaaggg caactaacaa caataatgct ttgaaccagg
    11281 tgctgcgggg cattcacgga cctcataatc aactttattt tctggccaga ttcaacaggt
    11341 taacgcaaat catctgccag aatttaatct tgtgctgcac gagtcagcct atgtttatat
    11401 aaccatcagt ccgtgactgg tgcgcatcat aaagtaagcg gatagattgc gcaattttta
    11461 tacagcgctc atgactgatt aaagcgaaac agcggtaaca ggaaacgttg cgactgttca
    11521 acgattgcct ccattgtctc tggtgttaat tgccgccaga gcctttgata ccagatatca
    11581 tcaccttcgc cactcaccat catgttgcct tcgtaagcct gaaggaattt cgtacgggct
    11641 ttttgcaacg tggaatcgtc atccagcatg gcatcgtttt gcgcgtcata acaggttttt
    11701 agccacgcgc cgccactttc aggtaacacc agcaatggcg cggacatccc ttcacgatac
    11761 ccctcaatca gttgtgagag gtaatgcaaa gcctgttcgg ctgcaagcgg cggaaaacgc
    11821 cactcgccgt ctttgcgtag aaaaaggcga ctttcaccat taccaccgct ggcacagtag
    11881 acaaggtgtt ccagccaaag ttgcattccc tgcgccacac ttaataaaga gggacgccag
    11941 cgcaacaagc catccggctg cacctgcggc aaccagccag ttatctgcac accgttgcaa
    12001 gcgagatcaa tttccatact ctgccccggc tggcgacagg caatgactct gtcggcaagc
    12061 tgctgcatct cctggcactg tgtttcccag aaaatttcac caaaagcgcc atacggtaaa
    12121 tcccctgccg cccggaagcg gcggaacaag cgttcggcat catcctgctc aaccagtgca
    12181 ttcaataact gctgattgat ttgataacgg ctaagtcctt ccagaataaa tggctcggtg
    12241 tcggggattt cgctgtcttc agtacggaag ttcacctgca aacgcatctg gaagaacgcc
    12301 cgcaccggat gtgcccagaa tcgttgtagc gtttccagcg gcacggtttc cggtaaggta
    12361 aacggcagcg gctgaacaaa ttcagaatgt gctttaccag cctggctggc cgcaggtagc
    12421 cattcacgag catagctttg tcgttcgcct ggctggtagt tttgcggatc aaacggcatc
    12481 cgggtatgga ggcaagtaag atgcgctttt acccttgcct cgctttcatc acagttgagc
    12541 gcttcatcgc ccggtagata atgactttgc ccgatgtagt cgatcagttc ctgcaccagt
    12601 accgacggga aacgttcact gttatcctga atggaacgcc cgatatagct gatatagagt
    12661 ttttgctgcg cggaaattaa cgcttccagg aacaggtagc ggtcgtcatc gcgacggcta
    12721 cggtcgccac gcttcggttt ctggctcatc aggtcaaagc ccaatggcgc aagctgacgt
    12781 ggataaacgc cgtcgttcat tcccagcagg caaaccactt tgaacggaat tgaacgcatt
    12841 ggcatcagag tacaaatgtt aaccggcccg gcgagaaaac gctggctgat acgttcttga
    12901 tccaggcgct gtgccagttc atcacgcaat agtgacagcg gcaccgcatc gccatactgc
    12961 gcacctaaac cttcggcgat aatcgcctgc cattgttgtt cgatcagcgt catcgccgct
    13021 tcggtttccg catccggcag gaagagggcg ttgagcatat cgcgacaaac cggcaaccac
    13081 tcttccagcg gacgctcctg tgccagcccg cgacgccaga tgtttagctg cattagcagt
    13141 gaagccagat gccccaccag ttctgcaatt aagccgctcg attcatcata aggtagaacc
    13201 gattgccact cgccctgcgc gctctccatc gcgtagccca acaacatacg cgtcaggcca
    13261 aatcgccagg tgtgttgtcc ggtggcgggg agttccagct cgcgaacgtt gtcgtcatct
    13321 atcccccaac gaatgccgga ttcgttgacc cactgacgta aataacgcag cccttcttcg
    13381 gtgatgtcaa accgcgccgc cagcaccggc acatccagta atgccagcac gtcttccgac
    13441 acaaagcggc tgtctggcaa cgataacagg ctgataaacg cttcaagtac aggatgcgac
    13501 tgccgcgccc gacggtcgga aatggcgtaa ggcaggtaac gatccgcagg tgcgctacca
    13561 aacacagcct gaataaacgg actgtagctg tcgatatcag ccaccatcac gatgatgtcg
    13621 cgcggagtaa gtgtcgggtc ttcttccagc atcgccagca ggcgatcgtg taaaacttca
    13681 acttcacgct gcgggctatg gcaaacgtgg aaggtgatac tgctatccag tggatcaagc
    13741 ggacgtttgt tatcgctacg ggaaaactct tcgatgttca caccagcaac ggcgcggttt
    13801 tccagttcca gaatgtcaga ctgaatgtta tgcagcaggt tatctggcgt cacatcgaca
    13861 aaggcgtcca gctcctggct gctctccagg tcagaaagga gataaatgta gtcgcgccca
    13921 agcttgcccc atgaggccag cagcgggttg ccgacatcct gttcaccatc gctgttaaag
    13981 agctgcccgg cattttcgct gtcgcgaaat agcggtaatt cgcgatcttc aaaactgtgt
    14041 cggcgctggc gagtcagtag tttcgccaga taagcaggat ctttaatgtc gccccagtaa
    14101 taacggcagg ggttggtaaa caggagatgg atttcaatat gtttacccag cgcctggagc
    14161 gcctggagat aaacaggcgg taacgcggaa ataccgcata taaagacgcg cgaaggtaac
    14221 cccggcgggc aggtcgtcgc ggactccagc gtttcgataa agcgctgata gagattggcg
    14281 cggtgccagc gcggttgtcc gagttcatgg gtatattcca ccagcgcctt ccacaacggc
    14341 gcttgccagg cctgtgcctc tcccagccct tcaaccaaat gtcctgtttc ccactgtgcc
    14401 agccagtccg gacgatagac cagatactgg tcaaacaggt ccgccgcttt tgaggaaagc
    14461 tggaacagct ttcgcttgtc gctatcgtca gtcagataat gccgcaacag ggtaaagtct
    14521 tcgcgctcca gcaattgcgg cagcagagtc atcagtttcc agctcatgct ctgtttgtta
    14581 aaggcgctct ctttggggat ctccggtaac acccggacga acatatccca gataaagctc
    14641 gcaggcagcg gaaaatcaat gtttgccgca ataccaaact tttgcgacag ggtcatttgc
    14701 agccactgtg ccataccggt actttgcacc agaatcatct ctggttcgaa aggatcgtcc
    14761 agccgttcgc gttcgacaat aaactccatc aacgcttcca gcacgtccag acgattggaa
    14821 tggtagaccc ttaacatagc ggctcctgac tactgacgat tcgggcaatg caggcgggtc
    14881 atctcgcctg ctctgccccc gggtgaaact agcgtaacgc tgatgctgac acatcccgcc
    14941 tgcgatgtct gcattcggtt gacctgccag ttggcaggtg gcgaaatcgc gcgcaattgc
    15001 gtttgctgcc aaccatgccg ccagagctgt tggtactggt ttctgctggt aagactgttc
    15061 attaatgttc gctggaaacc cgataacgca gtgacaatca tcaccatcaa caccatcgcc
    15121 aacattacct ccggcaggct aaagccttgt tgattcttca gggaagctga cataacgccc
    15181 cctctttcag cggacaaaaa tcgctccagc cgcgtggcga aaaaacaatt tttccatcaa
    15241 tgacttcgcc tgttcgccac aacgaaacgc cttcataacc ggcaattaat aaggcttcat
    15301 tatcttccag taaacgcaaa caaacccggg caccagtggc agcgtacagt aagcactgaa
    15361 ctgcgggctg cgtctgccag gagtgcattt tcccccacgc caacgccgac tgaacgatgg
    15421 cctggcggcg cagtgactga ctttccatgc tcacgcgaga ggcaaaactg cgatcctgct
    15481 ggctcattcc ttgtaatagc aagctaccca aaaccagcag catcaggacc agagccagtg
    15541 acgaaacacc cttttcgcgg ttcacaggtt gaatcctgtc acgctatagc tggcatccac
    15601 cacggtttgc ggttcagact tactggcagc acgcatatta accgtcaaca ccagcgagaa
    15661 gccgctgaca tcctgacgta cgacctgaaa agtgtcgata atgatggcat ccggattagt
    15721 gactttatcc cagcccttac cttcacagga tgtcgcaccg cgtagcgttt ccaacacttg
    15781 ctccttcaga cgaaatccaa tctggtcgga ctcttttacc ggttcgcgat cccagatacc
    15841 gttactgttc gcatcccact gcacaatgat acagtcaccc tgtccgacaa tttccagccc
    15901 ttcgccggta cagatgccat gacaataacc cgccctctgg agatgcttcg cgacggtaaa
    15961 tacccgcagc cagatttcat cttccagtgc cagcttacgg gtgctcgtta aactttcacg
    16021 ctgtaacgca ggcagaaagc gtgcagcccc caacaacaat acgctactga tcgccatagc
    16081 aatcaacact tccagcagag aaaaaccttg ctcttttaca ggcatccttc tgtttctcct
    16141 tgctgacaaa gccggagtct tccccacggc gaaaccacca gccaccactc gcccgttgag
    16201 tttttgaagc caatatgccc ggcccatgcg gtattgcgca ggccaaagaa agcaagcgaa
    16261 ggtgtcaggt cgctcatttc gacttcgggc cagcgtggca caaagaccaa tggtgaactg
    16321 ccatgacagg tattcgcccc agcaacggaa ctcacaaggc accataacgt cccctccctg
    16381 ataaaactga tactgtggtc gcggttatgc cagttggcat cttcacgtaa atagagcaaa
    16441 tagtcccgcg cctggctggc ggtttgccaa agccgttgcg actgctgcca gtattgccag
    16501 ccatagagtc cacttgcgct tagcatgacc aaaatcagca tcgcgaccag cgtttcaatc
    16561 agcgtataac cacgttgtgt tttcatgccg gcagtatgga gcgaggagaa aaaaagacga
    16621 gggccagttt ctatttcttc ggcgcatctt ccggactatt tacgccgttg caggacgttg
    16681 caaaatttcg ggaaggcgtc tcgaagaatt taacggaggg taaaaaaacc gacgcacact
    16741 ggcgtcggct ctggcaggat gtttcgtaat tagatagcca ccggcgcttt aatgcccgga
    16801 tgtggatcgt agccttcaat ctcaaagtct tcgaaacggt agtcgaagat ggattcgggt
    16861 ttacgtttga taatcaactt cggcagcgga cgcggttcac ggcttaattg cagatgagtt
    16921 tgatccatat gattactgta caggtgcgta tcgccaccgg tccagacaaa atcacccact
    16981 tccagatcgc actgctgcgc catcatatgc accagtaacg cgtagctggc aatgttgaac
    17041 ggcaggccga ggaagacgtc acaggagcgc tgataaagct ggcaagagag tttgccgtct
    17101 gccacataga actggaagaa tgcatggcac ggtgccagcg ccattttatc cagttcgcct
    17161 acgttccacg ctgaaacaat aatgcggcgg gaatccgggt cgtttttcag ctggttcagt
    17221 accgtagtga tctggtcaat atgacgacca tctggcgttg gccaggcgcg ccactgttta
    17281 ccatacactg gtccgaggtc gccgttttca tcggcccatt cgtcccagat ggtgacattg
    17341 ttttcgtgta gataagcaat gttagtgtcg ccctgcagaa accacagcag ttcatggatg
    17401 atggaacgca ggtggcaacg tttagttgtc accagcggga atccatcctg caggttaaaa
    17461 cgcatctgat gaccaaaaat ggaaagcgtt ccggttccgg tacggtcgtt tttctgtgtg
    17521 ccttcgtcga gcactttttg catcagttct aaatactgtt tcatggttcc tcaggaaacg
    17581 tgttgctgtg ggctgcgacg atatgcccag accatcatga tcacacccgc gacaatcatc
    17641 gggatggaaa gaatttgccc catgctgatg tactgcaccc aggcaccggt aaactgcgcg
    17701 tcgggctggc ggaaaaactc aacaatgatg cgaaacgcgc cgtagccaat caggaacaag
    17761 ccagacacgg ctcccatcgg gcgcggttta cgaatataca ggttgaggat aataaacagc
    17821 accacacctt ccagcagcag ctcgtaaagc tgtgatgggt ggcgcggcag cacaccgtaa
    17881 gtgtcgaaaa tggattgcca ctgcgggttg gtttgcagca gcaaaatatc ttctgtacgg
    17941 gagcctggga acagcatggc aaacgggaag ttcgggtcaa cgcggcccca caattcaccg
    18001 ttaataaagt tgcccaaacg cccggcacca agaccaaacg gaatgagtgg tgcgataaaa
    18061 tcagaaacct ggaagaagga acgtttagta cggcgggcaa agataatcat caccacgata
    18121 acgccaatca ggccgccgtg gaaagacatg ccgccgtccc agacacggaa cagatacagc
    18181 ggatcggcca taaactgcgg gaaattgtag aacagaacat aaccaatacg tcccccgagg
    18241 aagacgccga ggaagcccgc atagagtaag ttttcaactt catttttggt ccagccgctg
    18301 cccggacgat tcgcccgtcg tgttgccagc cacattgcaa aaatgaaacc caccagatac
    18361 atcaggccgt accagtgaag cgccacgggt cctattgaga aaatgaccgg atcaaactcc
    18421 ggaaaatgca gatagctact ggtcatctgt caccacaagt tcttgttatt tcgctgaaag
    18481 agaacagcga ttgaaatgcg cgccgcaggt ttcaggcgct ccaaaggtgc gaataatagc
    18541 acaaggggac ctggctggtt gccggatacc gttaaaagat atgtatatga tccgcgctat
    18601 aaccctccgc gaatcagccc gcccatgccg cgacgctcca taaaggctgc aacctgatgg
    18661 cgaacttcgg tcgccagttg cgcttccaga ctacgctgcg caagattttc tgcttcggca
    18721 taatcaatgc gccgcagcag gtattttacc cgcgctacag aacgtccgtt catagacaga
    18781 tggcgatacc caagcccaat gaggattgcc acgcacatgg gatcgcccgc catttcaccg
    18841 cacaaacgga gatcgattcc atgtatttcc gcttcccggg cgatcatcgc cagagctcgt
    18901 aacattgcag gatgaagact gtcataaatg ttcgccaccc gggtattgtt gcgatcaacg
    18961 gccagaatgt attgagtcag atcgttggtg ccaacagaga tgaaatcgac ccgctttgcc
    19021 agatgcggca gcataaatac cattgacggc acttccagca tgatgccgat acgtggtttg
    19081 ggaatttcgt aaccgatcat ctcctcgact tcacgtccgg cacgttcaat caggcggcgt
    19141 gcttcgtcaa cttcatcgag gcttgtgacc atcggcaaca gaatattcag gttgcccgta
    19201 gcggcattag cacgcagcat cgcccgcacc tggatcaaga agatctccgg ctgatcgagc
    19261 gtaatgcgaa tcccacgcca acccaggcat ggattctctt cgctaatcgg catgtaaggc
    19321 agctgcttat ctgctccgac atccagcgta cgcaaggtga cgggtttatc attgaacatt
    19381 tgcagcatcc cctgatactg cgccacctgt tcttcttccg acggaaaacc actttgcagc
    19441 atgaatggga tttcagtgcg ataaagaccg atgccatcaa tacggctgcc cagtttttct
    19501 tcatgttccg ggcttaaacc agcattgagc atgactttta tacgctcacc gctttttaac
    19561 tgcgcgggta aattgacgtc atcttccgcc agacggctaa gctcaatctc ttcactaatt
    19621 agccgctgat attcttgcag cagtaccggc tccggatcga ccagcaattc accgcgatag
    19681 ccatcgacga tcagcgtccg acgatgcagc accgaaggct gaatatccgc gcccatcacg
    19741 gtagggatcc ccagcgcacg taccatgatc gcagcatggg agttggctgc gccatctcgc
    19801 acgacaacac cgactaagcg atcctggggc agctcagcaa gagttgtcgc tgacagttca
    19861 tctgccacca gaatgaaacg ttccggccag gcgttcggcc cttgattagc gtcatcaaga
    19921 tgaaacagca atcgctgacc cagcgcacgt aaatcgccag cccgctcttt gagatagtta
    19981 tcgcttagcg cggcaaactg ttcggcaaat ttttcaatga ccgtttttac cgcccattct
    20041 gccaccgagc ctttatcaac ctcggcaaac aattcgcgac gcagccgggt gtccgaaagc
    20101 aggtgcgagt aaagatcgaa aatagccgcc gtttcttttt gtgcaccggc ggcaaagcgt
    20161 ttgctgtagc ggcgaaactc gtttgccgct tcttccagcg ccccggtcag tcgttcgcgt
    20221 tccagagccg gatccagcgt tgatgcctga tacacctgtt ccattaaagg taacgtggca
    20281 tcctgccagc cttcggcaat cgctacacca ggtgctgccg gtaatgcgcg gattcgcgtc
    20341 tggcgatatt gcccaaacaa ggcagtcaac tgcgactgag aaagaatcgc tgccatctgg
    20401 gtggcaagcg tcaccaggaa ggattcttca ctttcgtcat actggcgcaa ctctcgttgc
    20461 tgtaccacca gtacaccaag caactggcga cgttgaataa ttggtacgcc taaaaacgcg
    20521 cggaaacgtt cttcttttac ggaggggatg tatttgaagc tggggtgctt ttgcgcatct
    20581 gcaaggttta tcggttccgc cagcctgcca accaggccga cgatcccttc atcaaaggcg
    20641 agcgttacag tgcgaccgcg tggttttttc agcccccggg tcgccatcag gtagtaacaa
    20701 cgtcgatcat gatcggccag gtagaccgaa cagacctcgg tatccatcgc aagacagatg
    20761 tcggtaacca gaatatttaa cgcctcattc aggcgtggtg cgctggctac cttttcgact
    20821 atttcgcgca ggcgagtgag cataattggc gtgacttaac ctcttttacg tcgataagca
    20881 gatgcgtttt gtggttttgg cgtattttcc tgcagtgaca tcaccacact cgcgaactct
    20941 ttcattaccc tacggtagac atcacgttta aatgacacca cctgtctgac cggataccag
    21001 taacttaccc atcgccagcc gtcaaactct ggtgtactgc tggtttgcat attgatttct
    21061 gcatcgccgc tcaccagctg caagagaaac catttttgtt tttggccgat acaaaccggc
    21121 ttcgtgtccc aacgcaccaa acgtttcggt aatttgtagc gcaaccagtt acgcgttgaa
    21181 gcaaggattc gaacgtcttt gcggcttaat cctacttctt caaacaattc acggtacatc
    21241 gcctgctctg cggattctcc ggggttgatt ccgccttgcg gaaattgcca ggagtgctga
    21301 ccaaatcgcc gggcccacat tacctgcccc tggcgattac aaatcacgat acctacgttt
    21361 gggcggtagc catcgtcatc aatcaccgga ctacctcaaa ataaagcttt atatacgaat
    21421 gattgtttca tactccagga agacggtaaa ccactctctg cagggcatta cacactaaca
    21481 acaattgaat aactcacagt tatgtgcaga gttataaaca gaggaagggg tggatagccg
    21541 tttttattca ccttttctgt ggatagagtt gtgaagaact acggaattac tacgggaaaa
    21601 cccggagcat tctgaataag cccgatatag acattttaaa atattcatta ttagtcaatg
    21661 cattacatgt aatttaagta atgaaaaaga gtaattcgtg acccaggtca cacctctcat
    21721 ttacgggttg gcgaaagatc aaccaatgcc gtatttatcc acagaatgtg ccactaagtt
    21781 aagcactgaa ccactaaaaa ctggagtttc gtcgcacgtc aaggctgtaa atggaaacag
    21841 tagtggaggt ttttcacagt tatcccagct ttctgtggat aacatggtgt aagatcctgt
    21901 ttattttcag tgaccagatt tggaaaaccc gttgcagtgt tgcgcaactc gattaccggc
    21961 aacctaaaaa gcaatataaa tcagataatt gaacaacgca tgtggaaaaa gttacactgc
    22021 gaatattcgg cacataattg ctgtttgttt tttaaccaag gtatcatgac atgtcccaac
    22081 ctcgcccact gctctctcct cccgaaactg aagaacagtt gttagcgcaa gcacagcaac
    22141 tttctggtta tacattggga gaactggcgg cacttgccgg gctggttacg ccagagaatt
    22201 taaaacgcga taagggctgg attggcgtgt tactggagat ctggctaggt gccagcgcag
    22261 ggagtaaacc tgaacaagat tttgctgctc tgggcgtgga acttaaaact atccctgtgg
    22321 atagtcttgg tcgtccgctg gaaacaacat tcgtttgtgt tgccccgtta acgggcaata
    22381 gcggagtgac ctgggaaacc agccacgtgc gccacaaact caaacgcgta ctgtggatac
    22441 cggttgaagg cgagcgcagc atcccgctgg cgcagcgtcg cgttggatca ccgttgctgt
    22501 ggagcccgaa tgaagaggaa gaccggcagc tacgcgaaga ctgggaagaa ttaatggata
    22561 tgattgttct cggtcaggtt gagcggatta ccgctcgtca cggggagtat ttacagatac
    22621 gaccgaaagc agcgaatgcg aaagcgctta ccgaagccat tggtgcacgg ggcgaacgga
    22681 ttctgacgct gccgcgcggc ttttatttga agaagaattt caccagtgca ctactggccc
    22741 gtcattttct gatccagtag ccatcgcttt gacctgccgc tttccgggca tataattacc
    22801 gcttcatttt tttggcaggg ctttttagat gttatttgca tggataaccg atcctaacgc
    22861 ctggcttgcg ctcggtacgc tgacgctgct ggagatcgtt cttgggatcg acaatattat
    22921 tttcctttct ctggtggtgg caaagctccc cacagcacaa cgtgctcatg cgcgccgtct
    22981 ggggttggcg ggagccatgg ttatgcgtct ggcgctgctg gcatcaatcg cctgggttac
    23041 gcgcctgacg aatccgcttt ttacaatatt cagtcaggaa atttctgccc gtgacttgat
    23101 tctgcttctg ggtggcttgt tccttatctg gaaagccagc aaggaaatcc acgaatccat
    23161 tgaaggtgaa gaagaagggc tgaaaacacg cgtttcatca ttcctcggcg ctatcgtgca
    23221 gattatgctg ctggatatta tcttcagcct cgactcggtg attaccgctg tgggtctgtc
    23281 agatcacctg tttattatga tggccgccgt ggtgattgcc gtaggcgtga tgatgttcgc
    23341 cgcgcgctcg attggtgatt ttgtcgagcg ccatccttcg gtaaaaatgc tggcgctctc
    23401 tttcctgatt ctggtgggct ttaccctgat tctggaaagt ttcgacatcc acgtaccgaa
    23461 aggttacatc tacttcgcga tgttcttctc tattgcggtt gaaagcctca acctgattcg
    23521 caacaaaaag aacccgctct gatactccgt acgctctcct gcgggagggc gtactcactt
    23581 ctgttaccct cacagtttaa gattttcctg ctttcaagat atataacgtc agtttataaa
    23641 cagactatta tcataggtgg gccaaatgag gataaccgga tgaaaaaatg ggcagtaata
    23701 atttctgcag tcggactggc gtttgctgtt tccgggtgtt ccagtgatta cgtcatggcg
    23761 accaaagatg gccgtatgat tttgaccgat ggaaaacctg aaattgatga tgataccggg
    23821 ctggtgagtt atcacgatca gcaaggtaac gcgatgcaaa ttaaccgtga tgatgtttcg
    23881 caaataattg aacgttaaca aataaggtca gcatccggct ggccttaaga tttttctctt
    23941 tcccttttcc cttccctctg ccatttttat attccttatg tcgtgattat aaaaaggaaa
    24001 cggctatgca atatcaccgt ataccccaca gttcgctgga agtcagcacg ctggggcttg
    24061 gcacgatgac gtttggtgaa cagaacagcg aagccgacgc ccacgcacaa ctcgactatg
    24121 ccgtcgctca gggcattaac cttatcgacg ttgccgaaat gtacccagta cctccgcgcc
    24181 ccgaaacgca agggttaacc gaaacctacg tcggcaactg gctggcgaaa catggcagcc
    24241 gcgaaaagtt aattatcgcc tccaaagtga gcggaccgtc gcgcaataat gacaagggca
    24301 tccgcccgga tcaggcgctg gatcggaaga atatccgcga agcgctgcat gacagcctca
    24361 agcgtctgca gactgattac ctcgatcttt atcaggtgca ctggccgcag cgcccaacca
    24421 actgcttcgg caaactcggt tatagctgga cagattctgc gcctgcggtt tcgctgctgg
    24481 atacgctgga cgcactggca gagtaccaac gagcgggaaa aattcgttat atcggcgtgt
    24541 cgaacgaaac tgcatttggc gtaatgcgct acctgcatct ggcggacaaa cacgatctgc
    24601 cgcgtattgt caccattcag aacccttaca gtctgttaaa ccgcagtttt gaagtaggtc
    24661 tggcagaagt cagccagtat gaaggggtcg aactgctggc ctattcgtgc ctgggtttcg
    24721 gcacgctgac cgggaaatat ctcaatggtg caaaacccgc tggcgcacgt aatacgctct
    24781 ttagtcggtt cacccgctat agcggtgagc aaacgcaaaa agccgtcgcg gcgtatgttg
    24841 atatcgccag acgtcatggc ctggcccctg ctcagatggc gctcgcgttt gtacgccgtc
    24901 aaccgtttgt tgccagcact ctgctgggcg caaccacgat ggatcagctg aaaactaaca
    24961 tcgaaagttt gcatctggag ttaagcgaag acgtattagc tgaaattgaa gcggtgcatc
    25021 aggtttatac ttatccggca ccataagatt aacgccggtt aaaaccggcg ttaaatatta
    25081 atgacggcgc tgccagatcc acagcgccgt tattgccagc gcaaatagcg caccgaagcc
    25141 aatgccaatg ggcacgaccg ggatgcctac cattaccgcc agcgagtaaa tgcccagcat
    25201 caacaacatg gcgctgtttt cgccaaggtt ttgtactgca atcgcattcc ccgccccgac
    25261 gcttttttta ccccgctcct gtagcaacgc attgagcgga acgacaaaaa agccccccaa
    25321 cacgccaatc agcatcaaca aggcataggc tggcagctgc tcgtgttgca gggagaaaat
    25381 cagtaccacc acgccaatca aaattccggc tggcatacag cgtgacacgg tttccagcgt
    25441 aaccagcttc gctgccgcac ctgcgccaac cacgatacca atcgctacca tcgcgttgag
    25501 ataggtcggc gtagcgttat cggtaatgcc cagcgccacc ggtacccaca gcaccaacag
    25561 gaaacgcagc gtgacacccg ctccccagaa taaactggtg cccaccagcg aaaaacgcgt
    25621 ttcaccattg cgccatagcg aggtgcaggc attcaggaaa ctgcgggtca tgttgatgag
    25681 attccaggac tgccccggac gcgccgccgc cagtttggga atgtagatat tggcaacgac
    25741 cgcaccaccg taggccagtg cgcatgcggc cagggcgacc aggacatgcc agtcagccag
    25801 cacaccaccg gctacggaac cgagcaaaat cgccgctatg gtagaagctt ccattaaacc
    25861 gttagctttt actaacttac tacccgtggt taattcgccg agaataccgt atttcgccgg
    25921 tgaataggct gcagcaccaa cacccaccag cgtatagccg agaaacggat tgataccaaa
    25981 gcagatactg gctgcgccca gcagcttcag gccgttggca aacatcatca cccggccttt
    26041 ggcgaagcta tccgccacct gcccgacaaa cggcgcaaaa agaatgtaag cacctacaaa
    26101 caccatttgc aggatgggct ggctccactc cggatagaac tgcgctttca gtaacgccag
    26161 agtggcaaac agtagggcat tatcgccaaa cgcagagaga aactgcgcca cgataaccgc
    26221 tttcatcccc ttcgaccaca acgaagtgtt agtgtgcact gactcactca tcgtgttgtt
    26281 ccacttcgtc tacccagctt ttcaacgtga caaagtcagg tttgccgctg ccaagtaatg
    26341 gcatctgttt caaatagcga atatcacgcg gtacagcaag ctccggcacg ccgtgctcac
    26401 gggcatactg ttgcaactta tcgcgcgtca gttcgttatc tgtggtgaaa agcaccagtg
    26461 cctcgccttt gctggcatcg ctcttaatcg cagtggcatg gactttatct ggcgaaacac
    26521 caagtgccag ttgttccacc atttccagcg acaccatttc gcctgcaatt ttggcaaagc
    26581 gttttgcgcg gccctgaatc tgcacaaagc cctgctcgtc aaaacgcaca atatcgccag
    26641 tgtcatacca gccgcgctcc atttcgccgc gaacattctc ggcggtgggc acttccagta
    26701 cacctggctt ctccacccgc agatagccgt tcattatgtt cggccctttc agttgcaggc
    26761 gtccgccctc ttcgatacca gggaccgaca acaggcgcgc atccattcct ggtagaatac
    26821 gccctaccgt accgggtttc gccgccatcg gtacgttgat agaaacgaca ggcgcgcatt
    26881 cggtcacacc gtagccttca aggatgcgca ggccaaattt atcctgccaa agctgtttgg
    26941 tactttcttg tagtttttct gcgcctgcca ccacatagcg tagacgatag aagtcatacg
    27001 ggttggcgaa acgcgcgtag tgaccgagga aagtcgaagt gccgaacaac acggtgcaac
    27061 tgcggtcata caccaactcc ggcacaatac ggtaatgcag cgggcttgga taaagaaaca
    27121 cttctgcacc tgtgagcagt ggcgtaaaca ggcctaccgt cagcccaaag gagtgaaaca
    27181 gcggtaacgc cgacataaag cgatcgttgg tggtgaagtc ggcaatcgtt ttaatctgct
    27241 cgacattcgc cagaatgctt ttatggctat ggacgacgcc ttttggatgg ccttcagaac
    27301 cggaggtaaa aaggatcagc gcctcttctt ccggctgctg tttaacctgt gccagacgcg
    27361 gcatcagcaa atgagcgaag atccatactt tgtcggcagt ggtgacatct gcttttaaat
    27421 cttccagata gacccagcgt acctgagtaa gttgctccgg cagatgccag agtttgcctt
    27481 tatcgagaaa ctggcgggaa gtgaagatgg ttttgatttc agccgccgta atagcactgg
    27541 tcagcccttt taccccagca gtgtagttca tcattgcggg aatgcggcga cgggcgatgg
    27601 ccccaaaaat cactgccgca ctgatgcctg cattgggcag cattaagccg atgcgttcgc
    27661 cttcaacact gtatttttca aggatgcgtc caacaaacag cgttttcgta agcaatttgc
    27721 gataggagtc tggggtaaaa ttgacgtctt cgacacattt cttccctgct ccgaagcggt
    27781 acattgcact cagtaaagat tcgtacagcg tttcacgcgg gcgcaccgcc attcgcgctt
    27841 ccatcattat ttgatgcagc atttcgccag cgattttgcg acggtcacgg gcacgcggcg
    27901 catccggcat ctccacctgc gttggtggca aaatatgcag agtaatttgc gggaacaagc
    27961 gacgtttaac cagacctttc aggcggctga agtgcgtaag ttccgcccct tcaatacgca
    28021 caggaataac cgttgcacca gacttcgccg cgataaaacc cgcgccatcg tagattttca
    28081 tcagcgagcc tgtggtggtg atgcgtcctt cagggaaaat caccactggt cggccctgtt
    28141 ccaccagacg taccagatgt ttaatcgcca taggttgcgt cgggtcgaga ggaacaaagt
    28201 cgataaatga tttcagccaa cgcatatacc actgttggct tattgaggtg taaacggcaa
    28261 acactggacg cacaggtaaa aacagtccaa gcaaaatgcc atcaataaaa gagacgtgat
    28321 taggcgtaat tagaacgcgc tcgcccttca gtgcctgggg gtcacccgta acgcgaacgc
    28381 gatacaaaac acggcacaaa tttcgaaaaa agctaaaaag cattccaact ccctttgctc
    28441 tgattcagta aaagcgaatg gagggagatt acacgagata aagaacgcga gcgacagtaa
    28501 attaggtgcg aaaaaaaacc tgcgcatccg cgcaggttgg tgcaagagac agggtacgaa
    28561 gagcgtaccg aataatctca ccaatcaata cctctgggat cttgattgtg gtctgcacga
    28621 cgactcttcg ccagcgagaa aacgcaaagg aatgaaggga aatgcaacga ggtgtgtaaa
    28681 ttgtcggtta ctgttacaga ttgatgaccg gcaaaaaaaa acctgcgcat ctgcgcaggc
    28741 tggtgtaatt catgtgctca acccgaagtt gacttcacct atcaatacct ctgggatcac
    28801 cactttagca acctgaagcc aaacgccacc agcggtcaat cgcaacagcg tttcgcaaag
    28861 tgtaaccaaa ggtttgcaat ctcttttttt gtcttactta tttgcgtttg gctcacattc
    28921 ccacgatgaa aacacgccgc cccttgaacc aacgggcgtt ttccgtaaca ctgaaacaat
    28981 gtaagcgttt acccactaag gtattttcat ggcgaccata aaggatgtag cccgactggc
    29041 aggcgtttca gtcgccaccg tttcccgcgt cattaataat tcacccaaag ccagcgaagc
    29101 ttcccggctg gctgtgcata gtgcaatgga gtctcttagc tatcacccga acgccaacgc
    29161 ccgtgcgctg gcgcagcaga ccactgaaac ggtcggtctg gtcgttggtg atgtttccga
    29221 tccgtttttc ggtgcaatgg tgaaagcggt cgaacaggtg gcttatcaca ccggtaattt
    29281 cttattgatt ggcaatggtt accacaacga acaaaaagag cgtcaggcca ttgagcaact
    29341 gatccgccat cgctgtgctg cgttggtcgt ccatgccaaa atgatcccgg atgccgattt
    29401 agcctcatta ttgaaacaaa tgcccggtat ggtgctgatc aaccgtatcc tgcctggctt
    29461 tgaaaaccgt tgtattgctc tggacgatcg ttacggtgcc tggctggcta cgcgtcattt
    29521 aattcagcaa ggtcataccc gcattggtta tctgtgctct aaccactcta tttctgacgc
    29581 cgaagatcgt ctgcaagggt attacgatgc ccttgctgaa agtggtattg cggccaatga
    29641 ccggctggtg acatttggcg aaccagacga aagcggcggc gaacaggcaa tgaccgagct
    29701 tttgggacga ggaagaaatt tcactgcggt agcctgttat aacgattcaa tggcggcggg
    29761 tgcgatgggc gttctcaatg ataatggtat tgatgtaccg ggtgagattt cgttaattgg
    29821 ctttgatgat gtgctggtgt cacgctatgt gcgtccgcgc ctgaccaccg tgcgttaccc
    29881 aatcgtgacg atggcgaccc aggctgccga actggctttg gcgctggcgg ataatcgccc
    29941 tctcccggaa atcactaatg tctttagtcc gacgctggta cgtcgtcatt cagtgtcaac
    30001 tccgtcgctg gaggcaagtc atcatgcaac cagcgactaa ccgcagttaa agcaattcca
    30061 gcgccagtaa ttcttcgatg gtctggcgac ggcgaatcaa ccgcgcctga ccattatcaa
    30121 acagaacttc tggtaacagc ggacggctat tgtagttgga tgacattgat gcgccatatg
    30181 cccctgtatc atgcagtacc agataatcac ctgccttcac ttccggcaag gcgcggggtt
    30241 caacatttcc cccttcctgc tgggtaaaga catcgcccga ttcacataac ggtccggcga
    30301 cgacggtttc caccgttggt gcgtgttcca gagaacgacc atcagctgcc agggcactga
    30361 tatggtggta actaccgtac attgccgggc gcatcagatc gttaaacccg gcatcaacca
    30421 gcacaaagtg gcggctaccc atttgtttga cgctccgcac ctgagtaatt aacacgccag
    30481 actgcgctac caggaagcga cccggttcaa tttccagttt cacagggtgg cccaaatggc
    30541 gggcgatttg ctcacgcgcg gcattccaca gaccataata atgttcggta tcaaccgcct
    30601 cttcaccctg ttgataagga atagaaagcc caccgcccgc agaaatagcc tgtaaatcct
    30661 gaccgaattc gatgacctga cgcaccatag caccacacac ctgttccaga tgggcataat
    30721 caacgccaga accaatgtgc atgtgaatgc cgaccagctg cagatgatga cgttgtatca
    30781 cgtccagtgc ggcgggcaga tcggtgtacc agataccgtg cttgctgttt tcgccaccgg
    30841 tattggtttt ttggctatgt ccgtgaccaa accccggatt aacgcgcagc catacccgat
    30901 gccctggcga aacctggccc agttggtcga acatatcaac agaacccgca ttcaccggaa
    30961 tttgcaattc actgacgcgt tcaagcgtcg cctgatcgat aacatctgcc gtaaaaacaa
    31021 tatcatcggg gtgcgtttgc ggattgtaac ccgccgccaa cgcacgctct atttcgccta
    31081 acgagacgga atccactttc acgccctgct cacgcattaa gcgcaaaata tgaatattgg
    31141 aacaggcttt ctgtgcaaag cgcaccacat caaactgttt cagcgctgca atctgccgac
    31201 gaataatttg cgcatcgtag acccacaccg ggcagccaaa ttcagcgggc aaacgcagca
    31261 gattttcggc ggtgagatcg gtatcggtgc tgaacagtga atgtggcata acaaactcca
    31321 gatgagtgct tttttatgat tacgccacat cataaaaaga ataaaaaata tcgatttatg
    31381 tcgagtctat gcaaaaatga tatggattac cggattgcga gagagcgcta atggccgccg
    31441 ttaacttacg tcatattgaa atttttcatg cggtaatgac cgccggaagc ctgactgagg
    31501 cagcacacct gctacacacc tcacagccaa ccgtcagccg cgaactggcg cgctttgaga
    31561 aggtgatcgg gctgaaattg tttgagcgcg tacgtggacg attacatcct accgtgcaag
    31621 gactgcgtct gtttgaagaa gtgcaacgat cctggtacgg actggatcgc attgtcagcg
    31681 ccgcagaaag tctgcgcgag tttcgccagg gagaactgtc tattgcctgc ctgccggtct
    31741 tttcgcaatc ttttttaccg cagctcctgc aaccctttct ggtacgttat cccgatgtca
    31801 gcttaaatat cgtgccccag gaatcaccgc tacttgaaga gtggctctcg gcccagcgtc
    31861 atgatttagg actcactgag acactccata cgcctgcggg aacagaacgt accgaattac
    31921 tctctttaga tgaattgtgt gtgttacctc cgggtcatcc gctggcggta aaaaaggtat
    31981 taacgccgga tgattttcag ggtgagaact acatcagcct ttcccgtact gacagctatc
    32041 gccagttgct ggatcagcta tttactgaac atcaggttaa acgacgcatg atcgtagaaa
    32101 cccacagcgc cgcgtcagtc tgcgcaatgg tacgggcggg ggtaggtgtt tcggtggtta
    32161 acccgctcac cgcactggat tatgcggcaa gcggtttagt ggtgcggcgg ttcagtattg
    32221 cggttccgtt caccgtcagc ctgatccgcc ccctgcaccg cccgtcatca gcgctggtgc
    32281 aggcgtttag tgagcattta caagcggggt taccgaaact ggtcacttct cttgacgcta
    32341 ttttgtcgtc agctacgaca gcataaaagc gacagcatcc tcggcatgga tcgccgcagt
    32401 atcaaacaca ggcaaaacac tgcgctcttc tggcaccagt aaaccaattt ctgtgcagcc
    32461 aaaaatgacg ccctgtgcgc cctgttctgc aaggcgagca atcacttgcg cataataagc
    32521 gcgtgacgct tcggtaaatt gccccagaca cagttcttca aaaataatct gattaatttt
    32581 cgcccgttca tccgcttcag gaataagaca attgatggaa aattgttccg tcagccgccc
    32641 gcgataaaaa tcctgttcca tggtgtaacg cgtacccagc agcgccacac gagtcattcc
    32701 ggccccggta attgcacgtc cggtggcatc cgcaatgtgt aagaaaggca gagtgcaacg
    32761 tgactcaatg gcatccgcca ctttatgcat cgtattggta cataacacaa taccttctgc
    32821 gcccgcccgc tgtaagccaa gcgccgcctc agccagaatg tccccggttt tatcccattc
    32881 cccgcgacgc tggcactctt ctatttcatg aaaatcgacg ctatgtagca gcacttgcgc
    32941 agagtgaagc ccaccaagcc gctgtttaat gccttcattt atcagacgat agtaaggaat
    33001 ggtggattcc cagctcattc ctcccagcaa accaattgtt ttcattcctc tctcctgatg
    33061 tgtgttaccg caacaaggct gaataaggaa cgagacaaac gcctcaacgg ccaggtgccc
    33121 gatctctatt aacgaaaaaa gggccggatg tacagcacat ccggcccgtg aaatcagacg
    33181 ccgatatttc tcaacttctc gcctgccatc agtttgcgtt cgatatgttc cagcgtgaca
    33241 tttttggttt ccggaatgag ccagaaagtg atgcccacaa acgcaatgtt cagcgcagtg
    33301 tagagccaga acgtaccggc agcgccaatg ctatcaagca gtgtcaggaa ggtcgcgccg
    33361 ataatcatat tcgacaccca gtttgtcgtc gtcgaacagg taataccgaa atcgcggcat
    33421 ttcagcggct gaatttcaga gcacaggatc cacaccactg gcgcggcgct catcgcataa
    33481 ccggcaatac acatcatcgt catgccaaca gagagccagg acaagccact ggaagccgta
    33541 ccgttatcaa actgcatcag gcaatagccc agcaccagag tgcctaacgc catcacgctg
    33601 aaaccaattt tcagagccgg tttacgacct gctttatcta ccgtaaagac cgcaatgaag
    33661 gtcgcgaaca taaaggtcag tcccacgacc agagtcgcaa tcatctgttg ttctgtggtc
    33721 gtaaagcccg ccattttgaa gatacgcggc gcatagtaca tgatgatgtt cataccggta
    33781 aactgctgca tcgcctgcaa caacataccg agaaacacag cacgacggac gttacggttg
    33841 atcttaaaca gtgcccaacc gccttgtttt aacttcaggc tttcacgaat ttcgttgagt
    33901 tcttctcgcg ctttttccga cgtatcgcgc agcatacgca acacttcttc cgcctcaata
    33961 tgacgcccct tttccgccag ccagcgcggg ctatttggca ggaagaccac cagaataatc
    34021 agcaggactg ctggtaaagc aagaaccccc aacattgcgc gccagttacc gctataactg
    34081 aacgctgtat cggataaaaa cgccagcacg atgccgagtg tgaccatcaa ctggtacata
    34141 ctgatcatct taccgcgaac gttttcactt gccatttcag aaagatacag aggagcggtg
    34201 taagacgcga tcccgacagc aatgcccagc accacacgag cggcgattaa catctctacg
    34261 ctggtcgcaa aagcggaccc tatagaactg agtacaaaca ggatggcccc cgccatcagg
    34321 ctgtatttac gccccaggcg gaacgacagc caaccattaa acagcgcacc aattgctgcg
    34381 ccgagcatca tgctactaac cacccattcc tgcaaacgac tggtcagcac aaagtgatcg
    34441 gtaatgaacg gcaacgctcc ggcgattacg ccgatatcaa gaccaaataa caatcctgcg
    34501 accgcagcag ctaccgaaac aaacatattc atacgccgcg tatcacgcaa agaacgtggc
    34561 gttaaagcag attccgtatt gatagtaacc attttttcct gccagcagag agtaagacat
    34621 agtgaaaaaa tacgtgaaca attcacacag gtgtcaggtc ggaaacagca taaatatgga
    34681 ttaaattgct gcgacatgcc gttatgtgat ggatattcca attttaaaat taagttgaat
    34741 tattgagatt attattaacc acctaatttt aaagcagata aaattcataa agttcatt
//
LOCUS       DBFIFM010000026        34488 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.026, whole genome shotgun sequence.
ACCESSION   DBFIFM010000026 DBFIFM010000000
VERSION     DBFIFM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.026"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            1..638
                     /locus_tag="N7C74_001134"
                     /pseudo
     CDS             1..638
                     /locus_tag="N7C74_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="toxin"
     gene            complement(788..2152)
                     /gene="mnmE"
                     /locus_tag="N7C74_001135"
     CDS             complement(788..2152)
                     /gene="mnmE"
                     /locus_tag="N7C74_001135"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HGU2431046.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSAKTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(2258..3904)
                     /gene="yidC"
                     /locus_tag="N7C74_001136"
     CDS             complement(2258..3904)
                     /gene="yidC"
                     /locus_tag="N7C74_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HGU2431047.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3907..4164)
                     /gene="yidD"
                     /locus_tag="N7C74_001137"
     CDS             complement(3907..4164)
                     /gene="yidD"
                     /locus_tag="N7C74_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HGU2431048.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFNTREH"
     gene            complement(4128..4487)
                     /gene="rnpA"
                     /locus_tag="N7C74_001138"
     CDS             complement(4128..4487)
                     /gene="rnpA"
                     /locus_tag="N7C74_001138"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HGU2431049.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVANLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(4504..4644)
                     /gene="rpmH"
                     /locus_tag="N7C74_001139"
     CDS             complement(4504..4644)
                     /gene="rpmH"
                     /locus_tag="N7C74_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HGU2431050.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4874..4954)
                     /locus_tag="N7C74_001140"
     CDS             complement(4874..4954)
                     /locus_tag="N7C74_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2431051.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            5251..6654
                     /gene="dnaA"
                     /locus_tag="N7C74_001141"
     CDS             5251..6654
                     /gene="dnaA"
                     /locus_tag="N7C74_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HGU2431052.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKSVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            6659..7759
                     /gene="dnaN"
                     /locus_tag="N7C74_001142"
     CDS             6659..7759
                     /gene="dnaN"
                     /locus_tag="N7C74_001142"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HGU2431053.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7759..8832
                     /gene="recF"
                     /locus_tag="N7C74_001143"
     CDS             7759..8832
                     /gene="recF"
                     /locus_tag="N7C74_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HGU2431054.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8861..11275
                     /gene="gyrB"
                     /locus_tag="N7C74_001144"
     CDS             8861..11275
                     /gene="gyrB"
                     /locus_tag="N7C74_001144"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HGU2431055.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(11299..11397)
                     /locus_tag="N7C74_001145"
                     /pseudo
     CDS             complement(11299..11397)
                     /locus_tag="N7C74_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11515..11913
                     /gene="yidB"
                     /locus_tag="N7C74_001146"
     CDS             11515..11913
                     /gene="yidB"
                     /locus_tag="N7C74_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HGU2431056.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQPVSGEQLESALGTNAVSDLGQKLGVDTSTASCLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            12028..12840
                     /gene="yidA"
                     /locus_tag="N7C74_001147"
     CDS             12028..12840
                     /gene="yidA"
                     /locus_tag="N7C74_001147"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HGU2431057.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGNTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12886..13542)
                     /gene="yidX"
                     /locus_tag="N7C74_001148"
     CDS             complement(12886..13542)
                     /gene="yidX"
                     /locus_tag="N7C74_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HGU2431058.1"
                     /translation="MKLNFKGFFKAASLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVANDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13678..13791
                     /locus_tag="N7C74_001149"
                     /pseudo
     CDS             13678..13791
                     /locus_tag="N7C74_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13788..14852)
                     /gene="cbrA"
                     /locus_tag="N7C74_001150"
     CDS             complement(13788..14852)
                     /gene="cbrA"
                     /locus_tag="N7C74_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HGU2431059.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRYLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKINAAYWHATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            14953..16167
                     /gene="yidR"
                     /locus_tag="N7C74_001151"
     CDS             14953..16167
                     /gene="yidR"
                     /locus_tag="N7C74_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HGU2431060.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRVSQGAHVGVVTVHPKSEKYVFIHGPENPHETWHYDFHHRRGVIVE
                     GGKMNNLDAMDITVPYTSGALRGGSHVHVFSPNGEMVSFTYNDHVMHQFDSALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGIVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLGNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(16169..16501)
                     /gene="yidQ"
                     /locus_tag="N7C74_001152"
     CDS             complement(16169..16501)
                     /gene="yidQ"
                     /locus_tag="N7C74_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HGU2431061.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    16741..16813
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            16807..17220
                     /gene="ibpA"
                     /locus_tag="N7C74_001153"
     CDS             16807..17220
                     /gene="ibpA"
                     /locus_tag="N7C74_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HGU2431062.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            17332..17760
                     /gene="ibpB"
                     /locus_tag="N7C74_001154"
     CDS             17332..17760
                     /gene="ibpB"
                     /locus_tag="N7C74_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HGU2431063.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            17956..19617
                     /gene="yidE"
                     /locus_tag="N7C74_001155"
     CDS             17956..19617
                     /gene="yidE"
                     /locus_tag="N7C74_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HGU2431064.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(19614..20330)
                     /gene="yidP"
                     /locus_tag="N7C74_001156"
     CDS             complement(19614..20330)
                     /gene="yidP"
                     /locus_tag="N7C74_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU2431065.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPDKS"
     gene            20626..22242
                     /locus_tag="N7C74_001157"
     CDS             20626..22242
                     /locus_tag="N7C74_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGU2431066.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            22242..23564
                     /locus_tag="N7C74_001158"
     CDS             22242..23564
                     /locus_tag="N7C74_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HGU2431067.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGSR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMHQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSASVAKAILDELLEANKAYWPELR"
     gene            complement(23561..24454)
                     /gene="yidL"
                     /locus_tag="N7C74_001159"
     CDS             complement(23561..24454)
                     /gene="yidL"
                     /locus_tag="N7C74_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGU2431068.1"
                     /translation="MNGKLQTSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPIGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            24621..26336
                     /gene="yidK"
                     /locus_tag="N7C74_001160"
     CDS             24621..26336
                     /gene="yidK"
                     /locus_tag="N7C74_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HGU2431069.1"
                     /translation="MNSLQILSFVGFTLLVAIITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRMIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFLRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            26333..27826
                     /gene="yidJ"
                     /locus_tag="N7C74_001161"
     CDS             26333..27826
                     /gene="yidJ"
                     /locus_tag="N7C74_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGU2431070.1"
                     /translation="MKRPNFLFIMTDTQAPNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALKPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDAHFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(27873..28322)
                     /gene="yidI"
                     /locus_tag="N7C74_001162"
     CDS             complement(27873..28322)
                     /gene="yidI"
                     /locus_tag="N7C74_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HGU2431071.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVERKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGARVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            28431..28778
                     /gene="yidH"
                     /locus_tag="N7C74_001163"
     CDS             28431..28778
                     /gene="yidH"
                     /locus_tag="N7C74_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HGU2431072.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            28768..29130
                     /gene="yidG"
                     /locus_tag="N7C74_001164"
     CDS             28768..29130
                     /gene="yidG"
                     /locus_tag="N7C74_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HGU2431073.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            29127..29624
                     /gene="yidF"
                     /locus_tag="N7C74_001165"
     CDS             29127..29624
                     /gene="yidF"
                     /locus_tag="N7C74_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HGU2431074.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDMTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRGCQACDWHLLCPHAREETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(29632..30816)
                     /gene="emrD"
                     /locus_tag="N7C74_001166"
     CDS             complement(29632..30816)
                     /gene="emrD"
                     /locus_tag="N7C74_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HGU2431075.1"
                     /translation="MKRHRNINLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            30898..30972
                     /gene="ysdE"
                     /locus_tag="N7C74_001167"
     CDS             30898..30972
                     /gene="ysdE"
                     /locus_tag="N7C74_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HGU2431076.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            complement(31096..31185)
                     /gene="tisB"
                     /locus_tag="N7C74_001168"
     CDS             complement(31096..31185)
                     /gene="tisB"
                     /locus_tag="N7C74_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HGU2431077.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            31750..31848
                     /gene="ivbL"
                     /locus_tag="N7C74_001169"
     CDS             31750..31848
                     /gene="ivbL"
                     /locus_tag="N7C74_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HGU2431078.1"
                     /translation="MATSMLNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            31954..33642
                     /gene="ilvB"
                     /locus_tag="N7C74_001170"
     CDS             31954..33642
                     /gene="ilvB"
                     /locus_tag="N7C74_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HGU2431079.1"
                     /translation="MASSGTSSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            33646..33936
                     /gene="ilvN"
                     /locus_tag="N7C74_001171"
     CDS             33646..33936
                     /gene="ilvN"
                     /locus_tag="N7C74_001171"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGU2431080.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            34199..>34488
                     /locus_tag="N7C74_001172"
     CDS             34199..>34488
                     /locus_tag="N7C74_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2431081.1"
                     /translation="MASPINSGIMMANLCPSTFLDKNRNVAAELDIKNNEKKYSPGSN
                     FAKWMLQEIKRLILNIMSGSRSINTDILDYFHPMPGTENNGNRTWVAATGED"
BASE COUNT         8322 a         8721 c         9124 g         8321 t
ORIGIN      
        1 gtttaaaaat gaattaaata aagaagatat attgaaactg gtggcagcaa gagaaaaagt
       61 ggcgggagca tcagacaaat ggacgaaggc atcaggctta tattctgcga tagtgaaagg
      121 gcatacgaag attgttgctg cctggatgga gacagctgaa gtgatagcca gccattatga
      181 aaatgataaa gatgtagtga gagaactcct gtcgctgagc agaaataatg cagtttgctc
      241 tttgtatgtt gccagctata agacaatgag taagcaggtc attgatgtat atctgaatgc
      301 ggcgattcgc ctggcgttgc aacacgggtt cactttcgat gagattttgg agcagtttac
      361 ccgtgacttt gatgggaagt cattctctct tgcggtagag aaagcggatg atatatatgg
      421 gtctctggct gaaaatattc aaaattgtgg ttggtgaaaa tgaaaattat cttaaagatg
      481 tgatgatgca actggaagcg aagaataatg aaggcaaata tgtaataagc aaggcaaatg
      541 gcaatcctgt atttaaagaa ctattctgga aagcaataga cgaatttaac tttccgcaag
      601 aggagcttaa tcggctaaag caatatcgtt ctctgtaaag acaggactgc aagatgttga
      661 aagtcgctcc agccagtagt aaaggcagtt ttggtgcagg atgctcataa aaaaacaagc
      721 gctgttccgt tctgatagtt agtttccccc agaacggaac agtgcccttt atatcaattt
      781 agacgactta cttaccaata cagaagctgg agaaaatccg ccccagcaaa tcatcagagg
      841 taaattcccc ggtgatttcg cttaagttct gctgtgccag gcgcaactct tccgccagca
      901 gttcgcccgc ccaggcaccc aacagttgcg ctttaccttg ctgcaaatgc tccgctgcct
      961 gttccagcgc ctgaaggtgg cgacgacgcg ccaggaagcc gccttccatg ttggtgtcaa
     1021 agcccatgct ctgtttgaga tggttacgca gcacgtccac gccttcgcca gtctttgccg
     1081 agagacgaat caacgcatga ccgttcactt cactcattcc cagcgtttcg ccagtgatat
     1141 cggctttatt gcgtaccacg gtgatcggca gtttcgcggg cagacgggcg ataaattccg
     1201 gccagatctc cgccggatca acggcgtctg tcgtggtgcc atcgaccata aacagcacgc
     1261 ggtcggcttg ttcaatttct tgccacgcgc gctcgatacc aatacgttcc acttcatcgc
     1321 tggcttcacg tagcccggcg gtgtcgatga tatgcagcgg cattccgtca atgtggatat
     1381 gctcacgcaa cacgtcacgc gtggttccgg cgatatcggt tacgattgct gcttcacggc
     1441 cagccagcgc gtttaacagg ctcgatttac cggcattagg acgtccggca atcaccacct
     1501 tcatcccttc gcgcaacaaa ctaccctgac gtgcttcagc acgcactgca tcgagatcgg
     1561 caatcacgtc attaagctgg gcttcaattt ttccgtcgga gaggaaatcg atctcttcat
     1621 cggggaaatc aattgccgct tcgacgtaaa tgcgcaagtg ggtgagggct tccaccagat
     1681 ggttgacccg tgcggagaat gcgccttgca gcgagttaag tgccgaacgg gctgcctgtt
     1741 ccgaactggc atcgataaga tcggcaatcg cctcggcctg ggctaagtcg agtttatcgt
     1801 taagaaacgc gcgttcggaa aactcaccag ggcgagcaat ccgcaggccg ggaatggtca
     1861 gaatgcgttt taacagcagg tcgagaatca ccggaccgcc atgaccctgc agttccagca
     1921 catcttcgcc ggtgaacgag ttcgggccag ggaaccatag cgcaatcccc tgatcgagca
     1981 cgctgccatc ggcgtcttta aacggaagat aatcggcgta gcgcggctta ggcagtttac
     2041 ccagcacggt ttcggcaact tcacgggctt tgaagccgga gatgcgcagg atgccaacgc
     2101 cgccacgtcc cggaggcgtg gcctgggcta cgatagtgtc attatcgctc atgatgttcc
     2161 tgttgctttg tgtggcggat acgcggtgct tatccgccct acgaaaagaa aaaaggcggt
     2221 caactgaccg cccttatttt agcgaaaact caccgaatca ggattttttc ttctcgcggc
     2281 tatgcaggcc acgtttttcc agaccacggt aaatcagctg ctgctgaata atggttacca
     2341 ggttgctgac gatatagtac agcaccagac ctgacgggaa ccacaggaag aacacggtga
     2401 agatgaccgg cataaaggtc atgatcttct gctgcatcgg gtcggtcaca gtggtcggcg
     2461 acatcttctg aatgaagaac atcgttacgc ccatcaggat cggcaggatg tagtacgggt
     2521 cctgtgcaga caggtcgtgg atccacagtg caaacggtgc ctgacgcagt tcaacggaac
     2581 ccatcagcat gtagtacaac gccaggaaga ttggcatctg gatcagcagc gggaagcagc
     2641 cgcccagcgg gttaaccttc tcagctttgt acagcgccat catttcctgg ctgatacgct
     2701 gtttgtcatc gcccagacgc tcacgcattg cctgaatctt cggctgcagc atacgcatct
     2761 tcgccatgga ggtgtactgc gctttggtca gcgggtacat gatgccacga acgataaagg
     2821 tgatgatgat aatggagaag ccccagttac ccacaaagct atggatccat ttcagcagtt
     2881 tgaacagcgg ctgagagatg aaccacaacc aaccgtaatc aacggtcaga tccaggtgcg
     2941 gagcaacagc tgccattttg tcctggattt ccgggccaac ccacagggtg ctgttcatcg
     3001 cgccagtctg accaggctga accagtaccg gctgagattt atagccgata gcggcgatgc
     3061 cgttacccag attagcagta tagaagttgt tggtaccgtc gttatgcggg atccacgccg
     3121 tcgcgaaata ctgttgcagc attgccaccc aaccaccttt cgaagagatg ttcaggtttt
     3181 cgttatcggc aatggtatcg aacttgtatt tctcgtactt ctcgtcaggc gtggagtacg
     3241 ccgcgccacg gaaggtgtgc agtgcgaagt tgctgcttcc ggtatcgaga tgcggtggca
     3301 gagtgatgga ttgcttcaac tgaccaaagg tggagatttc cagcggtttc tcgccagcgt
     3361 tctgcacgtt gtagttgacg ttgacagcgt aatcaccccg tttcaggaca aacgttttgg
     3421 taaacgtgtt gcctgccgcg tcggtatacg tcatcggcac ctgcagttcg ttttgacctt
     3481 cagccagcac ataagcgtct ttttcaacgt tatacagcgg acgcgggccg ttagccgggt
     3541 tatctgggcc atcacgaccg gtcagaccgc tctgtgcctg ataaataaac tgcggtgaag
     3601 tttccagcag ctggaacggc tgggtagagt tcagctcttt cgggtaagca ggcagcagag
     3661 cttgctcaac atcaccacca cgggtgttga tggtcagatc aagcacgtcg gtcttaaccg
     3721 agatcagttt cccctggcca ctggccggta cgccctggtc ggcggcgcta cccgctgcgg
     3781 tggtcgttgt ctgcgtggtc tgttgggcct gaggttgcgg gtttttatcc tgctcccagg
     3841 cttgccagat catgaaagac acgaacagca aagcgatgac taaaagattg cgttgcgaat
     3901 ccatcgttag tgttctctgg tattaaatgg tccgggcggg acgggatcgt caccaccagg
     3961 gtgtaaaggg tggcatttta atacgcgttt caccgtcaac caactgcctt ttatcactcc
     4021 aaacctgcgc aatgcctcaa ttccgtagct tgaacaggtt ggagtgaaac gacaatgcgg
     4081 cccgagtagc ggactaatca ggcgttgata gacccgaatg agggctatca ggacccgcga
     4141 gccaggcgac agtggcggcg ccataatttt tccaacgctt ccgagagagc acggttatcg
     4201 aggttggcaa cccctttttt cgccaccacc acgaaatcca tagccgggag ttcatgttgg
     4261 cgcagacgga agctttcacg cgtcagacgt ttaatccgat tgcgttcatg ggcgcgtcga
     4321 acgtttttct tggcgactgt aagaccgata cggggatgcc ccagcgaatt caggcggccg
     4381 agaatggtaa tttgcggcgt gccagcccgt tgtggctgct ggaagacgaa tgtgaattga
     4441 ctgggagtta acaagcgtaa ctccctggga aatgcgagct taaccactca ggggttagct
     4501 ttattactta gaaacggtca gacgagcgcg gcctttagca cgacgacgtg ccagaacctg
     4561 acgaccattt ttagtagcca tacgagcacg gaagccgtga gaacggttgc gcttcagtac
     4621 agacggttga aaagtgcgtt tcatggcgat ttctacctaa acttgaataa attcaatggc
     4681 tttattggat atccgccgaa aaatgaaacg atggacaccg aagccatggg tgattaaaga
     4741 ggccggattg taataattgt acactccgga gtcaattctc tttccttatt taccgcgctt
     4801 ttccgcacct tttcgcaggg aaaacgtacg gcctcacacc agtgaaaacc agcatggcgc
     4861 gccgggtgga ggattatacg ggctgatggg taaagcgcaa ggatcgtcct ggatctttat
     4921 tagatcgatt aagccaattt ttgtctatgg tcattaaatt ttccaatatg cggcgtaaat
     4981 cgtgcccgcc tcgcggcagg atcgtttaca cttagcgagt tctggatcat cctgtggata
     5041 aatcgggaaa atctgtgaga aacagaagat ctcttgcgca gtttaggcta tgatccgcgg
     5101 tcccgatcgt tttgcaggat cttgatcggg catataaccg cagacagcgg ttcgtgcgtc
     5161 accctcaagc agggtctttt cgacgtacgt caacaatcat gaatgtttca gccttagtca
     5221 ttatcgactt ttgttcgagt ggagtccgcc gtgtcacttt cgctttggca gcagtgtctt
     5281 gcccgattgc aggatgagtt accagccaca gaattcagta tgtggatacg cccattgcag
     5341 gcggaactga gcgataacac gctggccctg tacgcgccaa accgttttgt cctcgattgg
     5401 gtacgggaca agtaccttaa taatatcaat ggactgctaa ccagtttctg cggagcggat
     5461 gccccacagc tgcgttttga agtcggcacc aaatcggtga cgcaaacgcc acaagcggca
     5521 gtgacgagca acgtcgcggc ccctgcacag gtggcgcaaa cgcagccgca acgtgctgcg
     5581 ccttctacgc gctcaggttg ggataacgtc ccggccccgg cagaaccgac ctatcgttct
     5641 aacgtaaacg tcaaacacac gtttgataac ttcgttgaag gtaaatctaa ccaactggcg
     5701 cgcgcggcgg ctcgccaggt ggcagataac cctggcggtg cctataaccc gttgttcctt
     5761 tatggcggca cgggtctggg taaaactcac ctgctgcatg cggtgggtaa cggcattatg
     5821 gcgcgcaagc cgaatgccaa agtggtttat atgcactccg agcgctttgt tcaggacatg
     5881 gttaaagccc tgcaaaacaa cgcgatcgaa gagtttaaac gctactaccg ttccgtagat
     5941 gcactgctga tcgacgatat tcagtttttt gctaataaag aacgatctca ggaagagttt
     6001 ttccacacct tcaacgccct gctggaaggt aatcaacaga tcattctcac ctcggatcgc
     6061 tatccgaaag agatcaacgg cgttgaggat cgtttgaaat cccgcttcgg ctgggggctg
     6121 actgtggcga tcgaaccgcc agagttagaa acccgcgtgg cgatcctgat gaaaaaggcc
     6181 gacgaaaatg acattcgttt gccaggcgaa gtggcgttct ttatcgccaa gcgtctacga
     6241 tctaacgtac gtgagctgga aggcgcgctg aaccgcgtca ttgccaacgc caactttacc
     6301 ggacgggcga tcactatcga cttcgtgcgt gaggcgctgc gcgacttgct ggcattgcag
     6361 gaaaaactgg tcaccatcga caatattcag aagacggtgg cggagtacta caagatcaaa
     6421 gtcgcggatc tcctttccaa gcgtcgatcc cgctcggtgg ctcgtccgcg tcagatggcg
     6481 atggcgctgg cgaaagagct gactaaccac agtctgccag agattggcga tgcgtttggt
     6541 ggtcgtgacc acactacggt gcttcatgcc tgccggaaga tcgagcagct gcgtgaagag
     6601 agccacgata tcaaagaaga tttttcaaat ttaatcagaa cattgtcatc gtaaacctat
     6661 gaaatttacc gtagaacgtg agcatttatt aaaaccgcta caacaggtga gcggtccgtt
     6721 aggtggtcgt cctacgctac cgattctcgg taatctgctg ttacaggttg ctgacggtac
     6781 gttgtcgctg accggtactg atctcgagat ggaaatggtg gcacgcgttg cgctggttca
     6841 gccacacgag ccaggagcga cgaccgttcc ggcgcgcaaa ttctttgata tctgccgtgg
     6901 tctgcctgaa ggcgcggaaa ttgccgtgca gctggaaggt gagcggatgc tggtgcgctc
     6961 cgggcgtagc cgtttttcgc tgtctaccct gccagcggcg gatttcccga acctcgatga
     7021 ctggcagagt gaagtcgaat ttaccctgcc gcaggcgacg atgaagcgtc tgattgaagc
     7081 gacccagttt tctatggcgc atcaggacgt tcgctattac ttaaacggta tgctgtttga
     7141 aaccgaaggt gaagaactgc gcaccgtagc gaccgacggc caccgtctgg cggtctgttc
     7201 aatgccaatt ggtcaatctt tgccaagcca ttcggtgatc gtgccgcgta aaggcgtgat
     7261 tgaactgatg cgtatgctcg acggcggcga taatccgctg cgtgtgcaga ttggcagcaa
     7321 caatattcgc gcccacgttg gcgactttat cttcacctct aaactggtgg atggtcgctt
     7381 cccggattac cgccgcgttc tgccgaagaa tccggacaaa catctggaag caggctgcga
     7441 tctcctcaag caggcgtttg ctcgcgcggc aattctctct aacgagaaat tccgcggtgt
     7501 gcgcctgtat gtcagcgaaa accagctgaa aatcaccgcc aacaacccgg aacaggaaga
     7561 agcggaagag atcctcgacg ttacctatag cggtgcggag atggaaatcg gcttcaacgt
     7621 cagctatgtg ctggatgttc tgaacgcgct gaaatgcgaa aacgtccgca tgatgctgac
     7681 cgattcggtt tccagcgtgc agattgaaga tgcagccagc cagagcgcgg cttatgttgt
     7741 catgccaatg agactgtaat gtccctcacc cgcttgttga tccgcgattt ccgcaacatt
     7801 gaaaccgcgg atctcgcttt atctcccggc tttaactttc tggtaggtgc caacggcagt
     7861 ggcaaaacca gcgtgctgga agccatctat acgctcggcc atggtcgggc gtttcgcagt
     7921 ttgcagattg gtcgcgtcat tcgccacgag caggaggcgt ttgttctcca cgggcgatta
     7981 cagggcgaag agcgcgagac ggcgattggc ttaaccaaag acaaacaggg cgacagcaaa
     8041 gtccgcatcg acggtactga cgggcataaa gtcgcggaac tggcgcacct gatgccaatg
     8101 cagctgataa cgccagaagg gtttacttta ctcaacggcg gccccaaata cagaagagca
     8161 ttcctcgact ggggatgctt tcacaacgaa cccggatttt tcaccgcctg gagcaatctc
     8221 aagcgattgc tcaagcagcg caatgcggcg ctgcgccagg tgacacgtta cgaacagcta
     8281 cgcccgtggg ataaagaact gatcccgctg gcggagcaaa tcagtacctg gcgcgcggag
     8341 tatagcgccg gtatcgcggc cgatatggct gatacctgta agcaatttct ccctgagttt
     8401 tctctgactt tctctttcca gcgcggctgg gagaaagaga cagaatatgc tgaggtgctg
     8461 gaacgtaatt ttgaacgcga tcgccagcta acctacaccg cgcatggccc gcataaagcg
     8521 gacttacgca ttcgcgccga cggtgcgccg gtggaagata ccttatcgcg tgggcagctt
     8581 aagctgttga tgtgcgcctt acgtctggcg caaggagagt tcctcacccg tgaaagcggg
     8641 cggcggtgtc tctacctgat agatgatttt gcctctgagc ttgatgatga gcgtcgcggg
     8701 ctgcttgcca gccgcttaaa agctacgcaa tcgcaggtct ttgtcagcgc gatcagtgct
     8761 gaacacgtta tagacatgtc ggacgaaaat tcgaagatgt ttaccgtgga aaagggtaaa
     8821 ataacggatt aacccaagta taaatgagcg agaaacgttg atgtcgaatt cttatgactc
     8881 ctccagtatc aaagtcctga aagggctgga tgcggtgcgt aagcgcccgg gtatgtatat
     8941 cggcgacacg gatgacggca ccggtctgca ccacatggta ttcgaggtgg tagataacgc
     9001 tatcgacgaa gcgctcgcgg gtcactgtaa agaaattatc gtcaccattc acgccgataa
     9061 ctctgtctct gtacaggatg acgggcgcgg cattccgacc ggtattcacc cggaagaggg
     9121 cgtatcggcg gcggaagtga tcatgaccgt tctgcacgca ggcggtaaat ttgacgataa
     9181 ctcctataaa gtgtccggcg gtctgcacgg cgttggtgtt tcggtagtaa acgccctgtc
     9241 gcaaaaactg gagctggtta ttcagcgcga gggtaaaatt caccgtcaga tctacgaaca
     9301 cggtgtaccg caggcaccgc tggcggttac cggcgagact gaaaaaaccg gcaccatggt
     9361 gcgtttctgg cccagcctcg aaaccttcac caatgtgacc gagttcgaat atgaaattct
     9421 ggcgaaacgt ctgcgtgagt tgtcgttcct caactccggc gtttccattc gtctgcgcga
     9481 caagcgtgac ggcaaagaag accacttcca ctatgaaggc ggcatcaaag cgttcgttga
     9541 atatctgaac aagaacaaaa cgccgatcca cccaaatatc ttctacttct ccaccgaaaa
     9601 agacggtatc ggcgttgaag tggcgctgca gtggaacgat ggcttccagg aaaacatcta
     9661 ctgctttacc aacaacattc cgcagcgtga cggcggtact cacctggcag gcttccgtgc
     9721 ggcgatgacc cgtaccctga atgcctacat ggacaaagaa ggctacagca aaaaagccaa
     9781 agtcagcgcc accggtgacg atgcgcgtga aggcctgatt gcggtcgttt ccgtaaaagt
     9841 gccggacccg aaattctcct cccagaccaa agacaaactg gtttcttctg aggtgaaatc
     9901 ggcggttgaa cagcagatga acgaactgtt ggcggaatac ctgctggaaa acccaactga
     9961 cgcgaaaatc gtggttggca aaattatcga tgctgcccgt gcccgtgaag cggcgcgtcg
    10021 cgcgcgtgaa atgacccgcc gtaaaggtgc gctcgactta gcgggcctgc cgggcaaact
    10081 ggcagactgc caggaacgcg atccggcgct ttccgaactg taccttgtgg aaggggactc
    10141 cgcgggcggc tctgcgaagc aggggcgtaa ccgcaagaac caggcgattc tgccgctgaa
    10201 gggtaaaatc ctcaacgtcg agaaagcgcg cttcgataag atgctctctt ctcaggaagt
    10261 ggcgacgctt atcaccgcgc ttggttgtgg tatcggtcgt gacgagtaca acccggacaa
    10321 actgcgttat cacagcatca tcatcatgac cgatgcggac gtcgacggct cgcacattcg
    10381 tacgctgctg ttgaccttct tctatcgtca gatgccggaa attgttgaac gcggtcacgt
    10441 ctacatcgct cagccgccgc tgtacaaagt gaagaaaggc aagcaggaac agtacattaa
    10501 agacgacgaa gcgatggatc agtaccagat ctctatcgcg ctggacggcg caacgctgca
    10561 caccaacgcc agcgcaccgg cattggctgg cgaagcgtta gagaaactgg tgtctgagta
    10621 caacgcgacg cagaaaatga tcaaccgcat ggagcgtcgt tatccgaaag caatgctgaa
    10681 agagcttatc tatcagccga cgctgacgga agccgacctc tctgatgagc agaccgttac
    10741 ccggtgggtg aacgcgctgg tcagcgaact gaacgacaaa gaacagcacg gcagccagtg
    10801 gaagtttgat gtccacacca atgccgaaca aaacctgttc gagccgattg ttcgcgtgcg
    10861 tacccacggt gtggatactg actatccgct ggatcacgag tttatcactg gtggcgaata
    10921 tcgtcgtatc tgcacgctgg gtgagaaact gcgtggcttg ctggaagaag atgcgtttat
    10981 cgaacgtggc gagcgtcgtc agccggtagc cagcttcgag caggcgctgg actggctggt
    11041 gaaagagtcc cgtcgcggcc tctccatcca gcgttataaa ggtctgggcg agatgaaccc
    11101 ggaacagctg tgggaaacca ccatggaccc ggaaagccgt cgtatgctgc gcgttaccgt
    11161 taaggatgcg attgccgctg accagttgtt caccacgctg atgggcgacg ccgttgaacc
    11221 gcgccgtgcg tttattgaag agaacgccct gaaagcggcg aatattgata tttaatggcg
    11281 ttaacaatgc gagcgtgcct gatgcgctac gcttatcagg cctacgagac tcctgcaata
    11341 tattgaattt gcatgttttt gtaggccgga taaggcgttc acgccgcatc cggcaagaag
    11401 ctataagaaa agggcggaga tcatctccgc cctttttatt tctgcaatcc gcaattatca
    11461 tcccgcagtt ctatactttt tgggtcaaca cgtaacgctt tacaggtgat tatcatgggg
    11521 ctttttgatg aagttgtcgg cgcctttctg aaaggcgatg cggggaaata tcaggcgatt
    11581 ttaagttggg ttgaggagca gggtggcatt caggtgctgc tggaaaaact gcaaagtggc
    11641 ggcttagggg ccattctctc aacctggctg agtaatcaac agggcaatca accagttagc
    11701 ggcgagcaac tggaatcagc actcggcaca aacgcggtat ccgatcttgg acaaaaactt
    11761 ggcgtggata ccagtacggc ttcctgttta ctggcagaac aattgccgaa gattattgat
    11821 gcgctctcac cgcaaggtga agtgtcacca caagccaata acgatctgct ttccgcaggc
    11881 atggaactgc tgaaagggaa actcttccgc taagcaaatg gggagcatgc aacccgcgtt
    11941 gcatccccct tttccctcgg gtgttgtact gatttttgag cggaatcgcg ttagcatggg
    12001 tcaggaacca atctacctgg ggaactcatg gctatcaaac tcattgctat cgatatggat
    12061 ggcacccttc tgctgcccga tcacaccatt tcacctgccg ttaaaaatgc gattgccgca
    12121 gctcgcgccc gtggcgtgaa tgtcgtgcta acgacgggtc gcccttatgc aggtgttcac
    12181 aattacctga aagagctgca tatggaacag ccgggcgact actgcattac ttataacggc
    12241 gcgctggtac agaaggccgc tgatggtaac accgtggcgc aaactgctct cagctatgac
    12301 gactatcgtt tcctggaaaa actctctcgc gaagtcggtt ctcatttcca cgccctggac
    12361 cgcaccacgc tgtacaccgc caaccgtgat atcagctact acacggtgca tgaatccttc
    12421 gttgccacca ttccgctggt gttctgcgaa gcggagaaaa tggaccccaa tacccagttc
    12481 ctgaaagtga tgatgattga tgaacccgcc atcctcgacc aggctatcgc gcgtattccg
    12541 caggaagtga aagagaaata taccgtgctg aaaagtgcgc cgtacttcct cgaaatcctt
    12601 gataaacgcg ttaacaaagg tacgggtgtg aaatcactgg ccgacgtgtt aggtattaaa
    12661 ccggaagaaa tcatggcgat tggcgatcag gaaaacgaca tcgcaatgat tgaatatgca
    12721 ggcgtcggtg tggcaatgga taatgctatt ccttcggtga aagaagtggc gaactttgtc
    12781 accaaatcca accttgaaga tggcgtggcg tttgctattg agaagtatgt gctgaattaa
    12841 tctgtgggcg ggcaaacagc ataaacgcgt ttgcccgctt actgattatt ggtttactgc
    12901 atatacaacc agaaattgca gcggcgcagt aatgatgtcc agcgtaacag tcactggcag
    12961 taatgctgcg gaaacaccat ccggaatgcc tctgggtgaa tagtattcat aaaatcgcac
    13021 tttaaatgga tggtagaacg ccatcacctt tgagtaatcc atgtttttat ttttctcatg
    13081 gattgtgcct tttaggtttt ccagtgacct ggtacagtgt tgaacaccac aggcaaaacc
    13141 ataatgcgtt gccagtgcct tttcttcttc gttattccag tagtagtaaa gcgatattgt
    13201 gccggtaaat ttctttttcc ccgcatttaa aacaaatctt gcgtcattgg caacctgaat
    13261 attgtgccgg ttaagtgctg gatcattgag catcttaacg atgtcatcgg caccatcagt
    13321 gataaggtaa tccagcgatt cccctacaaa aacatagcct tttgttccat tactatcttt
    13381 tgcctgcgat agcccagtga tggtgtctga ctgcgattga tactttcctg aactaccctt
    13441 tgcggtatgg gaaaccagag catacgagat acagcctgaa agcatcagcg ccagtgggaa
    13501 taaactggca gccttaaaaa atcccttaaa attcaacttc atgattaatc ctttaaacga
    13561 gtagctaagc cgttgaaagc atactcttct ttccctcctg tttagcgcct gatctccctt
    13621 ttcagatttc tgcccgatat aagacctttt cttatgaggt gtttttttgt ggactaatat
    13681 ctgttgcaag gctatgggtt cgcacctgca ctggtttata tctccgccgt cgcgttgatt
    13741 ggcgcgctct cttacatcct gctggtgggc gatgtgacgc gcgttggcta atctttcaac
    13801 tgtggaatat gcgccatacc actgcgcata atccactttc ttaaagccgg tgcggtcagg
    13861 catcggcttt ttactatctt gccgaagagt tttaaacgca gtttgcgggt ggcgtgccag
    13921 taggctgcgt tgatcttctc tggctgcttc agtaacaccg aacgcagaat ctctgcgcta
    13981 tccagcgcat agcttatccc ctccagcgag ctggcgctga taaatcccgc cgcttcacca
    14041 atcagaaagg cgttgtcctt accgcagaca aaatcctgcc agcgtgaggg aaatagcacc
    14101 gtgcattttt cgctttttac cgccttacca aactggaatt gaaaggcgct cattttctct
    14161 ttcagcgtcg tgaaacgcgt ctgaccgtct ttcattggat aggcaccgcc aaagataaaa
    14221 taaccgtctt tgctgatact ccatgagtaa cagtcagtta tcgcattatc aaagatgcat
    14281 gagtagaacg gcaccggatg tttctctgcg aaccactgct ggatagcgac atatttacga
    14341 atctgatggt ccgggtagag atatcgccgc accatcgagt tggcaccatc tgcaccgacc
    14401 agatagcggg cagtaatatg ctgctcccaa ccgtctgcac gaaaaatgac atgccattta
    14461 tcatcctcac gccagatttt ccggcacagg ctatcgtgat aaacctcaac gctggcgggg
    14521 atcagcgatt tcatccacaa gtcgaaagca tggcgattaa tattgatata gcttcgctgg
    14581 tagttacgcg tgagcgatgc ggcgacgtcg acagttttga cgctgaaaat ctgtggattg
    14641 gcgatcacat cgacaggaag cgtcagtcca tcgcgaataa aagatcgctg cgcgtccggt
    14701 gccagcagac cgccacaagg cttgctgaaa ccttcagtac catgctggtg ctttttatcc
    14761 agcgcgatca cctgcatttt gccggctaac tttcgtgcca acgccgaccc tgccgggccg
    14821 aggccaataa tcgccacgtc gaaatgttcc atcttttcac tcacattcat cacgatggca
    14881 aaagactatt gtctgaaagt gaagtccttg tgaagtatga tggcgggaac cgttttgtat
    14941 caggaccgcg ccatgaaaca gataaccttt gctccccgta atcacctgct caccaatacc
    15001 aatacctgga cgcccgacag ccagtggctg gtatttgacg tgcgtccttc tggcgcgtcg
    15061 tttaccggcg agaccattga gcgtgtgaat atccataccg gcgaggtcga ggttatctat
    15121 cgcgtgtcac agggcgcgca cgtcggcgtg gtgaccgttc atccaaagtc agagaaatat
    15181 gtctttatcc acggacctga aaatcctcat gaaacatggc attacgattt ccatcaccgt
    15241 cgcggcgtga ttgttgaagg cggcaagatg aacaatctcg atgcaatgga tattaccgtt
    15301 ccgtacacat caggggcgct gcgtggcggc agccatgtgc atgtctttag cccgaacggt
    15361 gaaatggtga gttttaccta taacgaccat gtaatgcatc agttcgattc ggcgctggat
    15421 ttgcgaaacg tcggggttgc tgcaccgttt ggcccggtca acgtacaaaa gcagcatccg
    15481 cgtgaataca gcggtagcca ctggtgcgtg ctggtgagta aaaccacgcc cacgccgcag
    15541 cctggcagcg atgaaatcaa tcgtgcttat gaagaaggat gggtaggaaa tcacgcgctg
    15601 gcgtttattg gcgacacact ttcgccaaag ggcgagaaag tgccggagct gtttatcgtt
    15661 gagttaccgc aagatgaagc tggctggaaa gcagcaggtg atgcgccgtt aagtggaacg
    15721 gaaacaaccc tgcccgcgcc accgcgtggc atcgtgcagc gacgtttaac ctttacccac
    15781 catcgggctt atccggggtt agtcaacgtc ccgcgccact gggtgcgctg taatccgcag
    15841 ggtacgcaaa tcgcgttttt aatgcgagat gataacggca ttgtgcaact gtggcttatc
    15901 tcgccacagg gcggcgagcc gcgccagtta acccataaca aaacggatat tcagtctgca
    15961 tttaactggc atccgtcagg agaatggttg ggctttgtgc tgggtaatcg aattgcttgt
    16021 gcccatgcgc aaagcggcga ggttgagtat ttaaccgaaa accacgccaa tccaccttct
    16081 gcggacgccg tggtcttctc gccggatggt cagtggctgg cgtggatgga aggcggccag
    16141 ctgtggatca ccgaaactga tcgctaaatc agttatcagg cattctggcc ggtggaataa
    16201 ccgcattggt ggcttgcgcg ttggcttcgc ttttttcaac gcgtgagcgc accgagctgt
    16261 ctttgcggaa cacatcccac ggtaataaaa gcgtgtccat cacagcggta aacggcatat
    16321 cgagaatcgc cagcgactta gtgccccagt tggtttcatc atcgccaatc atggtggcgc
    16381 tggcgcgggt gccgggatac gttccttctt tccctccggt atgggacatc acgctggagc
    16441 atcctccgag taatgccatt ccgctgcata tcatgaacgc taacaacaca tttcttatca
    16501 taataaattc atctgttgat cgtgggtgtt ggcctgatga gttatagcga tcccttgctg
    16561 aaaataacat catcattacg tcgctctgtg gcggctatcg cactttaacg tttcgtgctg
    16621 ccccctcagt ctatgcaata gaccataaac tgcaaaaaaa agtccgctga taaggcttga
    16681 aaagttcatt tccagaccca tttttacatc gtagccgatg aggacgcgcc tgatgggtgt
    16741 tctggctacc tgacctgtcc attgtggaag gtcttacatt ctcgctgatt tcaggagcta
    16801 ttgattatgc gtaactttga tttatccccg ctttaccgtt ctgctattgg atttgaccgt
    16861 ttgtttaacc acttagaaaa caaccagagc cagagtaatg gcggctaccc tccgtataac
    16921 gttgaactgg tagacgaaaa ccattaccgc attgctatcg ctgtggctgg ttttgctgag
    16981 agcgaactgg aaattaccgc ccaggataat ctgctggtgg tgaaaggtgc tcacgccgac
    17041 gaacaaaaag agcgcaccta tctgtaccag ggcatcgctg aacgcaactt tgaacgcaaa
    17101 ttccagttag ctgagaacat tcatgttcgt ggtgctaacc tagtaaatgg tttgctgtat
    17161 atcgatctcg aacgcgtgat tccggaagcg aaaaaaccgc gccgtatcga aatcaactaa
    17221 tttcctaagg ccgccttgcg cggcctgaca tctccatgct cgccgtcagg gagcatatgc
    17281 gaatcttcgg atttgcaggt acttactcgc ttcttagaag gagaaatgac tatgcgtaac
    17341 ttcgatttat ccccactgat gcgtcaatgg atcggttttg acaaactggc caacgcactg
    17401 caaaacgccg gtgaaagtca gagcttcccg ccgtacaaca ttgagaaaag cgacgataac
    17461 cactaccgca ttacccttgc gctggcaggt ttccgtcagg aagatttaga gattcaactg
    17521 gaaggtacgc gcctgagcgt aaaaggcacg ccggagcagc caaaagaaga gaaaaaatgg
    17581 ctgcatcaag ggcttatgaa tcagccattt agcctgagct ttacgctggc tgaaaatatg
    17641 gaagtctctg gcgcaacctt cgtaaacggt ttactgcata ttgatttaat tcgtaatgag
    17701 cctgaaccca tcgcagcgca gcgtatcgct atcagcgaac gtcccgcgtt aaatagctaa
    17761 ctagctattc tctttgcccc gccattctgg tggggctttt tttgtctttc ttcttcaaca
    17821 atgcgttaaa tttcgactgt ttaagatatt tcggcacgtt ctttcccgtg ataatgtgcg
    17881 ctataaggca aatctgcttc accgattcag ccataatccg tagcaattaa aatgttatct
    17941 caagagaaat ggacgatgag tgatatagca ttaacggtca gtattttggc tttggtggca
    18001 gtcgtcggtt tgtttatcgg caacgtcaaa tttcgcggca taggattagg tattggcggc
    18061 gtgctgtttg gtgggatcat cgtcggccat tttgtttctc aggcggggat gacattaagt
    18121 agcgatatgc tgcatgttat tcaggaattt ggcctgatcc tgttcgttta taccatcggg
    18181 attcaggtag ggccgggctt ctttgcctca ttgcgcgtct ccggattacg cctcaacctg
    18241 tttgctgttc tgatcgtcat catcggtggt ctggttaccg ccatcctgca taaactgttt
    18301 gatattccac tgccggtagt gctggggatt ttctccggtg cggtaaccaa tacgccagcg
    18361 ctgggggcag ggcagcagat tttgcgcgac ctgggtacac caatggaaat ggtcgatcag
    18421 atggggatga gttacgcgat ggcgtatcca ttcggcattt gcgggatatt gttcaccatg
    18481 tggatgttgc gggttatttt ccgcgtcaat gtcgagacag aagcccagca gcacgagtct
    18541 tcacgcacca atggcggcgc gctgatcaag actatcaata ttcgcgttga gaaccctaac
    18601 ctgcatgatt tagccattaa agatgtaccg attctcaacg gcgacaaaat tatctgctcg
    18661 cgtctgaaac gcgaagaaac cctaaaagtt ccttcgccag ataccattat ccaactgggc
    18721 gatttgctgc atctggtggg tcagccagcg gatttacata atgcgcaact ggtgattggt
    18781 caggaggtcg atacttcgct gtccacgaaa ggcactgatt tgcgcgtcga gcgtgtggtg
    18841 gtcaccaatg aaaacgtgct tggtaagcgt attcgcgacc tgcattttaa agaacgctat
    18901 gacgttgtta tctcgcgcct gaaccgtgcc ggggtcgaac tggtcgccag tggcgatatc
    18961 agcctgcagt tcggcgatat cctcaacctg gtggggcgtc cgtccgcaat tgatgccgtt
    19021 gccaatgtgc tggggaatgc gcagcaaaaa ctgcaacagg ttcagatgct gccagtgttt
    19081 attggcatcg ggcttggcgt attgttaggt tctattcccg tctttgtgcc aggattcccg
    19141 gccgcgttga aactggggct ggcgggcggt ccgctgatta tggcgttgat cctcgggcgt
    19201 atcggcagta tcggcaagct gtactggttt atgccgccaa gtgccaacct cgcgctgcgg
    19261 gaactgggga tcgtgctgtt cctctcggtc gttggtctga aatctggtgg ggattttgtg
    19321 aataccctgg tcaatggcga agggctaagc tggattggtt atggtgccct gatcaccgcc
    19381 gttccgctga ttactgttgg cattctggcg cggatgttag ccaaaatgaa ttacctgacc
    19441 atgtgcggga tgctggcagg ttccatgacc gatcctccgg cgctggcgtt tgctaataat
    19501 cttcatccaa ccagcggtgc ggcggcgctc tcttacgcca ctgtctatcc gttagtgatg
    19561 ttcctgcgca ttatcacccc ccaattactg gcggtgctct tctggagtat cggttaactc
    19621 ttgtccgggt ggagtcgccg taaatggtac tccacctggt attcgctggc atttctgaac
    19681 atcactgaat aattcaaaaa ctccccgctc tcgctatatg acaacgaggt gatccgcagc
    19741 agtggcgtgt gttccgctac cttcatttgc cgcgccagca gtctatcggc gagcaccggc
    19801 gtcaggcttt cataattacc gccaatcaaa atcccgcact cttgttcaat atattcaaac
    19861 ttcgaccctt ccagatgttg cagcgaaagg ttacggaaca gttttaccgg catatagctg
    19921 tcttccagca tcagcggttt cccttccaca aaacgaacgc gacgggagaa gtagatctgc
    19981 tcgttgattt gaatccgcaa ctggctggca attgccggag gcgcaggcat gatttcaaaa
    20041 atcagcacct ggctggtgac tgtttttccc tgccgtttta acacctccac cagcccggtc
    20101 aggctggcgg tttgatgcag cacatctttg cgcaccaggt aagtgccgct gccgtggcgg
    20161 cggaccacca gcccccacgc taccagcagg tcaatcgctt tacggatggt catccgcgat
    20221 accgcaaact cttccgccag ctttttttca ccgggaagaa ggctgtttag cgtgaaatcc
    20281 gcggaattaa gtcgaattct taaccgctcc gcaatgcttt tgtagatcat ctctagatct
    20341 cttttttgcc tgcaagcccg ggtaatgcgc ccacaagttc tgcttaatca attgaaaaaa
    20401 catctttttt ttaaagatgt gttcgatgct gtgtaccagt gctcacagag atctactttt
    20461 tcgcgaaaaa gtagatctct accgcccaac gaaaagcatg aaagcgatca cgaatcccat
    20521 tgggtcgtca tgttctgccg atcgcatctt cctatcctcg ctccaggcct gccgcataac
    20581 caatcaggct tcctacttac agaattgaga aaagaggatg tggaaatgct cagtcaaatt
    20641 caacgctttg gcggcgcgat gttcacgcca gtgctgctgt ttcccttcgc cgggattgtg
    20701 gtgggtcttg ccatcttgct gcaaaacccg atgtttgtcg gggaatcact gaccgatccg
    20761 aacagtttat tcgcgcaaat cgtacacatt attgaagagg gcggttggac ggtattccgt
    20821 aatatgccgc tgatttttgc tgtcggttta cccattggcc ttgctaagca agcgcagggg
    20881 cgtgcttgtc tggcggtgat ggtgagtttc ctgacctgga actatttcat caatgcgatg
    20941 ggaatgacct ggggaagcta cttcggcgtc gatttcactc aggacgcggt ggcaggtagc
    21001 ggtctgacaa tgatggccgg gattaaaacc ctcgatacca gcattatcgg cgcaattatc
    21061 atttccggca ttgtgacggc gctgcataac cgtctgttcg ataaaaaact gccggttttt
    21121 ctcggcattt tccaggggac gtcttatgtg gtgattatcg ccttcctggt gatgatcccc
    21181 tgtgcctggc tgacattgct cggctggcca aaagtacaaa tggggattga atctctgcaa
    21241 gcgttcctgc gttcggcggg tgcgcttggg gtgtgggttt acaccttcct cgaacgtatt
    21301 ctgatcccaa ccggtttaca ccacttcatc tacggaccgt ttatctttgg tccggcagct
    21361 gttgaaggcg gaattcagat gtactgggcg cagcatctgc aagagttcag tttgagcgcc
    21421 gagccgctga aatcgttgtt cccggaagga ggttttgccc tgcacggtaa ctcaaaaatc
    21481 tttggtgccg tgggcatttc tttagcgatg tacttcactg ccgcaccgga aaatcgggta
    21541 aaagtggcgg gtttgctgat tcccgcaacc ttaaccgcca tgctggtggg aattaccgaa
    21601 ccgctggaat ttaccttcct gttcatttca ccgttgctgt ttgcggtaca cgctgtgctt
    21661 gcggcctcaa tgtcgaccgt gatgtatctc tttggtgtgg tgggcaacat gggcggaggt
    21721 ctgattgacc aggttttacc gcaaaactgg atcccgatgt tcagcaacca cgcggatatg
    21781 atgctgaccc aaatcgccat tgggttgtgc tttaccctgc tgtacttcgt ggttttccgc
    21841 accctgattc tgcaattcaa catgtgcacg ccgggacgtg aagatgcgga agtgaaactc
    21901 tactcaaaag ccgaatacaa agcctcgcga ggccaaacca ccgcggcaga gccaaaaaaa
    21961 gagctggatc aggctgccgg tatcctgcaa gccctgggcg gggtcggcaa tatctccagc
    22021 attaacaatt gtgcgacgcg tctacgcatt gcactgcatg acatgtcaca aacgctggat
    22081 gacgaagtct ttaaaaagct gggagcgcac ggcgtcttcc gtagtggcga tgccattcag
    22141 gtgatcattg gtctgcatgt atcccagctg cgtgaacagc tcgatagctt aattaattct
    22201 catcaatcag cagaaaatgt tgccattacg gaggcagtat aatgaccaaa ttctcagtgg
    22261 ttgtcgcagg cggtggaagc acctttacgc caggcatcgt gttgatgctc ctggcgaatc
    22321 aggaccgttt cccgcttcgt gcactgaaat tttatgataa cgatggttcg cggcaggaag
    22381 tgattgccga agcctgtaaa gtcatcctta aagaaaaagc gccggatatt gcgtttagtt
    22441 acaccaccga tcctgaagtg gcgttcagcg acgttgattt tgtcatggcg cacatccgcg
    22501 tcggcaaata cccgatgcgc gaactggatg aaaaaatccc gctgcgccac ggcgttgttg
    22561 gtcaggaaac ttgcggaccc ggcggaatag cgtacggcat gcgttccatt ggcggcgtcc
    22621 tggaactggt ggattatatg gaaaaatatt caccaaacgc ctggatgctc aactactcca
    22681 acccggcagc aattgttgca gaagccacgc gtcgtctgcg cccgaatgcg aaaatcctca
    22741 acatctgtga catgccaatc ggtattgaaa gccggatggc gcaaattgtt gggctgcaag
    22801 atcgcaaaca gatgcgcgtg cgctactacg gcctgaacca ctttggctgg tggacatcaa
    22861 ttgaagattt gcagggcaac gacctgatgc accagctgcg gcaatatgtc tctaagcacg
    22921 gttatgttcc accgcagcaa gatacacata ctgaagcgag ctggaacgac acctatgcaa
    22981 aagcgcggga tgtccaggca ctggacccgg atacattgcc gaacacctat ctgaaatatt
    23041 atctcttccc ggattatgtc gttcagcatt ccaaccctga acatactcgc gcgaatgagg
    23101 taatggaaca tcgcgagaaa caggttttcg atgcttgccg cgccattacg gcggcaggaa
    23161 attcagcggc gggcaagctg gaaattgacg aacatgcgtc atacatcgtc gatctggcgg
    23221 cggcaattgc cttcaacact caggagcgga tgttgctgat tgtgcctaac aacggggcaa
    23281 tccataactt tgatgatgaa gctatggtcg agatcccgtg tctggtcggg cacaacggac
    23341 cagaaccact ggtggtcggc gatatcccgc agtttcagaa agggttaatg agtcagcaag
    23401 tggcggtgga aaaactggtc gtggacgcct gggaacagcg ttcatatcag cacctgtggc
    23461 aggcgattac gttgtcgaaa acggtaccga gcgcctcggt cgccaaagct attctggatg
    23521 agttgctgga ggccaacaaa gcgtactggc cagagttacg ttaattgtgt ggactggcat
    23581 ccgttgggtg ctggtccgta ttctttagca cggctgaagg cgcataacca aatttatgtt
    23641 taaaggcttt gctgaaatga aaggagtcga agaagttgag cgtatcagcg acttcgccaa
    23701 ccgagtttcc ttgttgcttt aatagcgata gcgccagatc cagacgcgca tcgaggtaat
    23761 attctttcgg cgtctttccg gtatagcgta aaaacagacg acgcaaccag gcttcgctac
    23821 aggggatcgt ggcagccata tcggctacgc tccagcgttg ttgcagactg gcatgtaaag
    23881 tggcaattaa tttttcaatt tgccgccgtt gtggatcttt tttaccgtct gcgtacataa
    23941 gacaaatcca ctgataaata attttcgtca gaaaagcgac tgccagatta ttttttattg
    24001 cttctggtga agttattaac tcagcaactt cggtgagttc ctgattataa atttcgccat
    24061 tgtaaataac gctttgctga ccgatgggaa tatccatcat actggtgggg gtaaattcca
    24121 tccagtattg ttcccagact aaaccttcac agtgataaga gtgaatatcc attggcttta
    24181 aaaatataat acagttgcca tgcagtgtta tttgttcgcc attctttaaa agaatcttcc
    24241 cacatccttg tacggtatac accgcaaccc atgagttggc gataagcggt ttcttcttgt
    24301 cccgtggcca gatcacccgg taatgctcgt cggcgtaggt atgccagagc gagatcagag
    24361 aaattccgct ggagatcaca ttttcgttaa tcaataaagg aatattgtat ggagtttcgt
    24421 tttttacatc tgaagtttgc aattttccat tcattttaat atccctccgg cgaattatgc
    24481 tcacaacctg aacttaacag aaaacaatcc gttcagggat acgacgtttg tattttaaga
    24541 atctgactgc ctgacccgac gcattttacc tctccctata ttcatgcgtc cgggacataa
    24601 acataaataa gggctatgag atgaattcgt tacaaatctt gagttttgtc ggttttacgc
    24661 tgctggtggc gatcatcacc tggtggaagg ttcgcaaaac agataccgga tcgcaacaag
    24721 gctattttct tgccggacgt tcactaaaag cgccggttat tgccgcttcg ttaatgttaa
    24781 ccaacctttc cacggaacaa ctggttggtc tttccgggca ggcctacaaa agcggcatgt
    24841 cggtgatggg ctgggaagtg acttcagcgg tgacgctgat cttcctcgcg ctaatctttt
    24901 taccgcgcta tctgaagcgc ggcattgcca ccatccccga ttttctggag gaacgttatg
    24961 ataaaacgac gcgtatgatc atcgacttct gcttcctcat tgccaccggc gtctgctttc
    25021 tgccgattgt tctctactcc ggcgcgttgg cgctcaacag cctgtttcac gtcggggaat
    25081 cgctacagat ttctcacggt gcggctatct ggctactggt aattttgctt ggtctggcgg
    25141 gaattttgta tgcggtgatc ggcggactgc gcgcaatggc agtggcggac tccatcaacg
    25201 gtattgggct ggttatcggc gggttgatgg tgccggtatt tggcttgatc gcgatgggca
    25261 agggcagctt tatgcagggc attgagcaaa ttaccaccgt tcacgccgag aaattaaact
    25321 cagtcggtgg cccgaccgat cccttgccga ttggcgcggc atttaccggt ttaattctgg
    25381 tgaacacctt ttactggtgt acaaatcagg gcatcgtgca acgcacgctg gcgtcaaaaa
    25441 gcctggcgga agggcaaaag ggggcgctgt taacggcggt gctgaaaatg ctcgacccgc
    25501 tggtactggt gctgccaggg ttgattgcgt ttcatctgta tcaggatcta ccgaaagccg
    25561 acatggccta cccgacgctg gtcaataacg ttctgccagt gccactggtg ggtttcttcg
    25621 gcgcggtgtt atttggtgca gtgatcagta ccttcaacgg ctttctgaat agcgccagta
    25681 cgttattcag tatgggtatt taccgtcgca tcattaacca gaatgccgag ccgcagcagc
    25741 tggtcaccgt cgggcgcaaa tttggtttct ttatcgctat cgtttcggtg ctggtagcgc
    25801 cgtggatcgc caacgcgccg caggggctgt atagctggat gaaacagctc aacggcattt
    25861 acaacgtgcc gctggttacc atcatcatta tgggcttttt cttcctgcgc atcccggcgc
    25921 tggcggcaaa agtggcgatg gggattggca taatcagcta catcaccatc aactatctgg
    25981 tgaagttcga cttccatttc ctctatgtgc tggcctgtac gttctgcatc aacgtggtcg
    26041 tgatgctggt gatcggtttt atcaaaccgc gcgccacgcc gttcaccttc aaagatgcgt
    26101 ttgcggtgga catgaaaccg tggaaaaacg tcaagatcgc gtcaattggc atcctgttcg
    26161 cgatgattgg cgtctatgcc gggctggctg aattcggcgg ctacggtacg cgctggttag
    26221 cgatgatcag ttatttcatt gccgccgtag tgattgtcta cctgattttt gacagctggc
    26281 ggcatcgtca cgacccagcc gtaaccttta ctcccgacgc gaaggatagc ctatgaaacg
    26341 ccccaatttt ctgttcatca tgaccgatac ccaggccccc aatatggtcg gttgctatag
    26401 cggtaaaccg ctgaatacgc aaaatattga tagtctggcg gcggaaggta ttcgctttaa
    26461 ttccgcctac acctgttcac cggtttgtac accggcgcgc gccgggctgt ttaccggtat
    26521 ctacgctaac cagtccgggc cgtggaccaa caacgtcgcg ccgggtaaaa acatctccac
    26581 tatggggcgc tactttaagg atgccggcta tcacacctgt tacatcggca aatggcatct
    26641 cgacggtcat gactatttcg gcactggcga gtgtccgccg gagtgggacg ctgattactg
    26701 gttcgatggg gcgaactatc ttagcgaact gacggaaaaa gagattagcc tgtggcgcaa
    26761 tggcctaaac agcgtcgaag atttacaggc gaaccatatt gacgaaacct tcacctgggc
    26821 gcaccgcatc agcaatcgag cggtggattt tctgcaacag cccgcgcgcg ccgaggaacc
    26881 cttcctgatg gtggtttcgt atgatgagcc gcatcacccg ttcacctgtc cggtggagta
    26941 tttagagaaa tacgctgatt tttactacga gctgggcgag aaagcacagg atgacctggc
    27001 gaacaaacca gaacatcacc gcttatgggc gcaggcgatg ccatcgccag tcggtgatga
    27061 cgggctttat caccatccgc tctattttgc ctgtaatgac tttgttgatg accaaatcgg
    27121 acgggtcatc aatgccttaa agccagagca acgtgaaaat acgtgggtta tttatacctc
    27181 cgatcacggc gaaatgatgg gcgcacataa gctgatcagt aaaggggcgg cgatgtatga
    27241 cgacatcacc cgcattccgc tgatcatccg ttcgccgcaa ggggagcggc gacaggtcga
    27301 tacgccagtc agtcatatcg atttactgcc gacaatgatg gcgctggcag atattgaaaa
    27361 accagagatt ctgccggggg aaaatatcct tgcagtgaaa gagccacgcg gcgtgatggt
    27421 ggaatttaac cgctacgaga ttgagcatga cagctttggc ggttttattc cggtgcgttg
    27481 ctgggtgacg gatgacttta aactggtgct caacctcttc accagtgatg aactttacga
    27541 tcgccgtaat gatccaaatg aaatgcataa cctgatcgat gatgcccatt ttgccgacgt
    27601 tcgcagcaaa atgcatgatg ccttattgga ttatatggac aaaattcgcg atccgttccg
    27661 cagttaccaa tggagcctgc gtccgtggcg taaagatgca cagccgcgct ggatgggggc
    27721 atttcgtcca cgcccacaag atggttattc gccagtggta cgcgactatg acaccggcct
    27781 gccgacacaa ggggtgaagg tggaagagaa aaaacagaag ttctgacgat aatgcccgct
    27841 gatgaacacc cggcgggcat tattcaggct gcttaaacca gtgaaccgcc agtcaggaag
    27901 gaaaagataa ggaatatcag caaaatgctg cacacgatgc cgataccaaa aagcaaggta
    27961 tggaaagcca gcgtcccgcc gccaaaaacc cgcgcgccgc gaatgcccca gcgattttcc
    28021 ttacgcatac cgccaataaa tgcgcaaagt agcgcagcaa tggccagtgc gataccgctt
    28081 tgatcgagga ttttatcgac atccacgtcc tcttttctct ccacggtggt aattttttcg
    28141 ccttttattc cggcgagaag acctctttta atttcagccg ttttgtccgc cacgatgctt
    28201 tcaagcgttg gcggtggtgc ggaaaaaggg ccgaatgaaa agtggataat ccccatcatt
    28261 aaagcgatgg caccgaatag catgcccaat gaactgattt tattttgcgc tatgataccc
    28321 atctgacctc cttgtagcgg atggcaaaag catatcagga aacgggcgga cgaaattctt
    28381 aaaaaaaacc gctaaaggta gagcgttagt gaaattgcaa aggagcaaca atgaaaattt
    28441 cccgcctcgg agaagcgcct gattaccgct tctcgctggc taatgagcgt acttttctgg
    28501 cgtggatccg tactgcacta ggatttctgg cggcgggggt tgggcttgat caacttgcgc
    28561 cagatttcgc cacgcccgtc attcgcgaac tgctggcgtt gttgttgtgt ctgttttccg
    28621 gcgggttagc gatgtatggc tacttgcgct ggttacgcaa tgaaaaagcg atgcgtctga
    28681 aagaggacct gccctacacc aacagcttat taatcatcag cttaatattg atggtcgtag
    28741 cagtgatcgt tatgggactg gtgttgtatg ccggatagcc gcaaagccag acgcattgcc
    28801 gacccagggc tgcagccgga gcgtacatca ctggcgtggt ttcgtaccat gctgggctac
    28861 ggcgcgttga tggcgttggc tatcaaacac aactggcacc aggcgggcat gttattctgg
    28921 atttcgattg gcatcctcgc catcgtggcg ctgatcctct ggcactacac tcgtaatcgc
    28981 aatttaatgg atgtcacgaa tagcgatttt tcccaatttc acgtagtccg tgacaaattt
    29041 ttgatctccc tcgcggtgtt atctctcgca atactgtttg ctgtaacgca tatacatcaa
    29101 cttatcgtat ttattgagag agtcgcatga caggaagtca ggtcattgat gctgaagagg
    29161 acaggcataa actggttgta gagtacaagg atgctctgca acctgccgat ttttatcaca
    29221 atttcaaaca acggggcatc cgctccgtac agcttattcc ccatcttgaa tttgatgacc
    29281 ggggcgatat gacggccgct tcggtgacag cagagctgtg ggggaaattt ttaattgccc
    29341 tgtttgagtg ttgggtgcgg gcagatatca gtcgtatctc gattgagctt tttgacgcca
    29401 cattacaaaa atggtgtggc agcgaaaacc cgcaaccgcg ccgtggctgc caggcgtgcg
    29461 actggcatct gctgtgtccg cacgctcggg aggagacgcc agacagcgtg ctttgtgcgg
    29521 gctatcaggc gttttattct tactctgcgc cgcatatgcg ggtgatgcgt gatctgataa
    29581 agcagcatcg ttctcccatg gagctgatga cgatgctgcg gtgacgtgag cttaaacggg
    29641 ctgcccctga tgcgacatcc gcgtcgccag cggtagccag cacagcacga tcaacaatcc
    29701 cattaaggtc atcaacaacc ccaggctgcc ctgaccggtt tgcggcaaca tcgcagagag
    29761 cgacgccagc acgccggaac caatgttttg tagaccgccg accagcgcgc cagccgtgcc
    29821 cgccaggaaa gggaacggct ccatcgcgcc gctggtcgcc agcggaaaca gcatcccggc
    29881 accgaaaaag aacagcgcgg cgggaacgag cagcgtccag acattcatca cgccaaacca
    29941 gtcggggatc cacatcagta agccagccag caggcagcag ataaccgact gccacatcaa
    30001 cgttgagaag cgtttattag gacgtccggc aaaccatgcc ccaaaaaatg ccgccggaat
    30061 cggcagaata aacaaaatac tgaccgtcat actgctcagc cctaacaccg cgcccatcag
    30121 cacgccggag caggcttcaa aggcggcaat cccggccaga ccgccaatca gcatcagcaa
    30181 ataacagtta aaaccgctgt taccgaaaag cgttttataa ctggtaagca ggcgcgtgcg
    30241 cggtgcgtcg accggacgcg tttccggcat ccagcgggcc atactgaagg tcacaccggc
    30301 acaaagtacc aacaaaaaga gataacaggc gcgccagttc cacatcgtat ccagcagacc
    30361 gccgattagc ggtgcgagca acggactgac aagaattccc atgtttaaca ggctgttagc
    30421 gtggcgcaac tgtgtccgtt catataaatc acgcggcaaa gtacgcgcca ttacgccgcc
    30481 aacgccggtg cccatcccct gcatcgcgct ggcggcaatc aatactgtca aactggaggt
    30541 cgtgaccgcg accagcgttg ccagcataaa aatggacatt ccgacgagga tcaccggtcg
    30601 gcgacccaca cggtcggaaa tcgggccata aaacagctgt gagacaccgt aagtcagcag
    30661 ataagcgccc attacgctct gcaccgcccc ttcacgaacg ttgagatcgc gcgccatatc
    30721 ggcaatagct ggaatataaa tggtttgcgc catctgaccg acggccacga gtaataccaa
    30781 catcaataac aaattgatgt ttctatgcct tttcattatc acggatgctt ttataaaaat
    30841 aattagaata agtcactacg cattcccata aatgcgggag aggaatctat catattgata
    30901 aatgttagcc agatatacgc caggaatggg gaattgttta gcggcagaat atgtaaacaa
    30961 aagcggcaat aaatgttgcc gggagggatg gtaggtcgga taagatgcgt cagcatcgca
    31021 tccgacacca acccacacgc taaatataaa atgggagcgg ttacccgctc ccctttagtg
    31081 cgacttgaat ctgaattact tcaggtattt cagaacagca tcaagcagtt gcagtgctgc
    31141 aacaatgagt ttgaggataa gaatggcgat atccaccagg ctcatacgcg tctcctgtgg
    31201 ttcaggagca ctgccagctg acgtaccttt ccgctgcctg ttgccagctg ttgtgcacgc
    31261 ggtatctcaa ggagaaacgt tgtgctcgtt gacataacac agtgtgctca cgcggggtta
    31321 aggcgctgac ggcaccaccc gtttcagcca ggacttagtg cgccgggtaa cgatgcggcc
    31381 cccgcataac acattgcgta cagtgatatt atagaagctt gctgtataaa taaacagtaa
    31441 tatttggaca aaacgcaaac tgtgatcgat actcaactat gcactaaata cgtcaaaatt
    31501 cgtgccgaaa ttgcgcgttt tgcgcggaac acgtatactt tctgtgttga cataatacag
    31561 tgtgctttgc ggttaccagc cgcgggcggc tgacgaaacc tcgctccggc ggggtttttt
    31621 gttacctgca gtacagtaca aaacgtgatc aacccctcaa ttttcccttg ctgaaaaatt
    31681 ttccattgtc tcccctgtaa agctgtgctt gtataaatat tgttaaacac aaaaccaaca
    31741 aggtccccaa tggctacttc catgctcaac gcaaaactac taccaactgc gccatccgcc
    31801 gcagtggtcg tcgtgcgtgt ggtggtggtc gtcggcaatg cgccgtaggg actggaacaa
    31861 cacacgattc caaaaccccg ccggcgcaaa ccgggcgggg tttttcgttt aagcacctcc
    31921 cggaaagtcg gcccagaaga aaaggactgg agcatggcaa gttcgggcac atcatcgacg
    31981 cgtaagcgct ttaccggcgc agaatttatc gttcatttcc tggaacagca gggcattaag
    32041 attgtgacgg gcattccggg cggttctatc ctgcctgttt acgatgcctt aagccaaagc
    32101 acgcaaatcc gccatattct ggctcgccat gaacagggcg cggggtttat cgctcaggga
    32161 atggcgcgca ccgacggtaa accggcggtc tgtatggcct gtagcggacc gggggcgact
    32221 aacctggtga ccgccattgc tgatgcgcgg ctggactcca tcccgctgat ttgcatcact
    32281 ggtcaggttc ccgcctcaat gatcggcact gacgccttcc aggaagtcga cacctacggc
    32341 atctctatcc ccatcaccaa acacaactat ctggtcagac atatcgaaga actcccgcag
    32401 gtcatgagcg atgccttccg tattgcgcaa tcaggccgcc ctggcccggt gtggatagac
    32461 attcctaagg atgtgcaaac ggccgttttt gagattgaag cacagcccgc ggtggcagaa
    32521 aaagccgtcg cgcccgcctt tagcgaagaa agcattcgtg acgcagcggc aatgattaat
    32581 gctgccaaac gcccggtgct ttatctgggc ggtggtgtga tcaatgcgcc tgcgagagtg
    32641 cgtgaactgg cggagaaagc gcagctgcct accacaatga ctttaatggc gctgggcatg
    32701 ttgccaaaag cgcatccgtt gtcgctgggt atgttgggga tgcacggtgt gcgcagcacc
    32761 aactatattt tgcaggaggc ggatttgttg attgtgctcg gtgcgcgttt tgatgaccgg
    32821 gcgattggca aaaccgagca gttctgcccg aatgccaaaa ttattcatgt cgatatcgac
    32881 cgcgcagagc tgggtaaaat caagcagccg cacgtagcga ttcaggcgga tgttgatgac
    32941 gtgctggcgc agctgatccc gcaggtggaa gcgcaaccgc gtgcagagtg gcaccagttg
    33001 gtagcggatt tgcagcgcga attcccgtgt ccaatcccga aagcgtgcga tccgttaagc
    33061 cattacggcc tgatcaacgc cgttgccgcc tgtgtcgatg acaatgcgat tatcaccacc
    33121 gatgtgggtc agcatcagat gtggacggcg caggcttatc cgctcaatcg cccacgccag
    33181 tggctgacct ccggcgggct gggcacgatg ggttttggcc tgcctgcggc gattggcgcg
    33241 gcgctggcga acccggatcg caaagtgttg tgtttctccg gcgatggcag cctgatgatg
    33301 aatattcagg agatggcgac cgccagcgaa aaccagctgg atgttaaaat cattctgatg
    33361 aacaacgaag cgctggggct ggtgcatcag caacagagtc tgttctacaa gcagggcgtt
    33421 tttgccgcta cctatccggg aaaaatcaac tttatgcaga ttgccgccgg attcggcctc
    33481 gaaacctgtg atttgaataa cgaagccgat ccgcaggctg cattgcagga aatcatcaat
    33541 cgccctggtc cggcgctgat ccatgtgcgc attgatgccg aagaaaaagt gtacccgatg
    33601 gtgccgccag gtgcggcgaa tacagaaatg gtgggggaat aagccatgca aaacacaact
    33661 catgacaacg tgattctgga actcaccgtt cgcaaccatc cgggcgtaat gacccacgtt
    33721 tgtggccttt ttgcccgccg cgcttttaac gttgaaggca ttctttgtct gccgattcag
    33781 gacagcgaca aaagccatat ctggctactg gtcaatgacg accagcgtct ggagcagatg
    33841 ataagccaaa tcgataagct ggaagatgtg gtgaaagtgc agcgtaatca gtccgatccg
    33901 acgatgttta acaagattgc agtgtttttt cagtaaggag cgacattaaa ccgctcgggg
    33961 gagttactga tgagcgattt ttgatagatc cgtgcagata tatagggtga aattgtgcca
    34021 gagaccatct caggcacaat ttattagccc aggacgttaa tttaagtcgc agcaccggta
    34081 acctgggggg gacagttagg gctaaaattt tcagcgatgg tgttaaattc tgattgctgt
    34141 ctataccagt ggtcatgacc agcattctat taattaatgg ttcatcgtga ggtatattat
    34201 ggcaagtcct ataaacagcg gaataatgat ggcaaatctc tgcccttcca cctttttgga
    34261 taaaaataga aatgtcgctg ctgagcttga tattaaaaat aacgagaaaa aatattcacc
    34321 tggcagtaat ttcgcaaaat ggatgttgca ggaaataaag cgcttaatac taaacatcat
    34381 gtccggttct cggagtatta acaccgatat tcttgactat ttccacccta tgccaggcac
    34441 ggaaaataat ggtaatcgta cctgggtggc ggcaactgga gaggatga
//
LOCUS       DBFIFM010000027        34245 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.027, whole genome shotgun sequence.
ACCESSION   DBFIFM010000027 DBFIFM010000000
VERSION     DBFIFM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.027"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..1125
                     /gene="proV"
                     /locus_tag="N7C74_001173"
     CDS             <1..1125
                     /gene="proV"
                     /locus_tag="N7C74_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HGU2431082.1"
                     /translation="QGLSKEQILEKTGLSLGVKDASLAIEEGEIFVIMGLSGSGKSTM
                     VRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMVFQSFALMPHMTVLDNTA
                     FGMELAGINAEERREKALDALRQVGLENYAHSYPNELSGGMRQRVGLARALAINPDIL
                     LMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGDRIAIMQNGEVV
                     QVGTPDEIINNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFGPRSALKL
                     LQDEDREYGYVIERGNKFVGAVSIDSLKTALTQQQGLDAALIDAPLAVDAQTPLSELL
                     SHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
     gene            1118..2182
                     /gene="proW"
                     /locus_tag="N7C74_001174"
     CDS             1118..2182
                     /gene="proW"
                     /locus_tag="N7C74_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HGU2431083.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2239..3231
                     /gene="proX"
                     /locus_tag="N7C74_001175"
     CDS             2239..3231
                     /gene="proX"
                     /locus_tag="N7C74_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HGU2431084.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            3423..4607
                     /locus_tag="N7C74_001176"
     CDS             3423..4607
                     /locus_tag="N7C74_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2431085.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISNKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            4731..5468
                     /gene="ygaZ"
                     /locus_tag="N7C74_001177"
     CDS             4731..5468
                     /gene="ygaZ"
                     /locus_tag="N7C74_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HGU2431086.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            5458..5793
                     /gene="ygaH"
                     /locus_tag="N7C74_001178"
     CDS             5458..5793
                     /gene="ygaH"
                     /locus_tag="N7C74_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HGU2431087.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            5884..6414
                     /gene="emrR"
                     /locus_tag="N7C74_001179"
     CDS             5884..6414
                     /gene="emrR"
                     /locus_tag="N7C74_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HGU2431088.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDHLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            6541..7713
                     /gene="emrA"
                     /locus_tag="N7C74_001180"
     CDS             6541..7713
                     /gene="emrA"
                     /locus_tag="N7C74_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HGU2431089.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQQMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            7730..9268
                     /gene="emrB"
                     /locus_tag="N7C74_001181"
     CDS             7730..9268
                     /gene="emrB"
                     /locus_tag="N7C74_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HGU2431090.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(9332..9847)
                     /gene="luxS"
                     /locus_tag="N7C74_001182"
     CDS             complement(9332..9847)
                     /gene="luxS"
                     /locus_tag="N7C74_001182"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HGU2431091.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     VWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(9997..11553)
                     /gene="gshA"
                     /locus_tag="N7C74_001183"
     CDS             complement(9997..11553)
                     /gene="gshA"
                     /locus_tag="N7C74_001183"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HGU2431092.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIDKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(11626..12054)
                     /gene="yqaA"
                     /locus_tag="N7C74_001184"
     CDS             complement(11626..12054)
                     /gene="yqaA"
                     /locus_tag="N7C74_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HGU2431093.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFCPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12051..12617)
                     /gene="yqaB"
                     /locus_tag="N7C74_001185"
     CDS             complement(12051..12617)
                     /gene="yqaB"
                     /locus_tag="N7C74_001185"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HGU2431094.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(12898..12974)
                     /locus_tag="N7C74_001186"
     tRNA            complement(12898..12974)
                     /locus_tag="N7C74_001186"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12938..12940),aa:Arg,seq:acg)
     gene            complement(13077..13153)
                     /locus_tag="N7C74_001187"
     tRNA            complement(13077..13153)
                     /locus_tag="N7C74_001187"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13117..13119),aa:Arg,seq:acg)
     gene            complement(13216..13292)
                     /locus_tag="N7C74_001188"
     tRNA            complement(13216..13292)
                     /locus_tag="N7C74_001188"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13256..13258),aa:Arg,seq:acg)
     gene            complement(13357..13433)
                     /locus_tag="N7C74_001189"
     tRNA            complement(13357..13433)
                     /locus_tag="N7C74_001189"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13397..13399),aa:Arg,seq:acg)
     gene            complement(13437..13529)
                     /locus_tag="N7C74_001190"
     tRNA            complement(13437..13529)
                     /locus_tag="N7C74_001190"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13493..13495),aa:Ser,seq:gct)
     gene            complement(13845..14030)
                     /gene="csrA"
                     /locus_tag="N7C74_001191"
     CDS             complement(13845..14030)
                     /gene="csrA"
                     /locus_tag="N7C74_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HGU2431095.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(14265..16895)
                     /gene="alaS"
                     /locus_tag="N7C74_001192"
     CDS             complement(14265..16895)
                     /gene="alaS"
                     /locus_tag="N7C74_001192"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HGU2431096.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFVVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(17023..17523)
                     /gene="recX"
                     /locus_tag="N7C74_001193"
     CDS             complement(17023..17523)
                     /gene="recX"
                     /locus_tag="N7C74_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HGU2431097.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(17591..18652)
                     /gene="recA"
                     /locus_tag="N7C74_001194"
     CDS             complement(17591..18652)
                     /gene="recA"
                     /locus_tag="N7C74_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HGU2431098.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(18732..19229)
                     /gene="pncC"
                     /locus_tag="N7C74_001195"
     CDS             complement(18732..19229)
                     /gene="pncC"
                     /locus_tag="N7C74_001195"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HGU2431099.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITLRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(19374..20459)
                     /gene="mltB"
                     /locus_tag="N7C74_001196"
     CDS             complement(19374..20459)
                     /gene="mltB"
                     /locus_tag="N7C74_001196"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HGU2431100.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            20714..21276
                     /gene="srlA"
                     /locus_tag="N7C74_001197"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             20714..21276
                     /gene="srlA"
                     /locus_tag="N7C74_001197"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: protein motif:HMM:NF015565.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            21273..22234
                     /gene="srlE"
                     /locus_tag="N7C74_001198"
                     /gene_synonym="gutE"
                     /pseudo
     CDS             21273..22234
                     /gene="srlE"
                     /locus_tag="N7C74_001198"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
     gene            22245..22616
                     /gene="srlB"
                     /locus_tag="N7C74_001199"
                     /gene_synonym="gutB"
     CDS             22245..22616
                     /gene="srlB"
                     /locus_tag="N7C74_001199"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HGU2431101.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            22620..23399
                     /gene="srlD"
                     /locus_tag="N7C74_001200"
     CDS             22620..23399
                     /gene="srlD"
                     /locus_tag="N7C74_001200"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HGU2431102.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFVRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            23505..23864
                     /gene="gutM"
                     /locus_tag="N7C74_001201"
     CDS             23505..23864
                     /gene="gutM"
                     /locus_tag="N7C74_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HGU2431103.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIADTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            23931..24704
                     /gene="srlR"
                     /locus_tag="N7C74_001202"
                     /gene_synonym="gutR"
     CDS             23931..24704
                     /gene="srlR"
                     /locus_tag="N7C74_001202"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HGU2431104.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDADIDPAFRQALEEKGIDVIITGESNE"
     gene            24697..25662
                     /gene="gutQ"
                     /locus_tag="N7C74_001203"
     CDS             24697..25662
                     /gene="gutQ"
                     /locus_tag="N7C74_001203"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HGU2431105.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(25659..27173)
                     /gene="norR"
                     /locus_tag="N7C74_001204"
     CDS             complement(25659..27173)
                     /gene="norR"
                     /locus_tag="N7C74_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HGU2431106.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            27360..28799
                     /gene="norV"
                     /locus_tag="N7C74_001205"
     CDS             27360..28799
                     /gene="norV"
                     /locus_tag="N7C74_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HGU2431107.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEKMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            28796..29929
                     /gene="norW"
                     /locus_tag="N7C74_001206"
     CDS             28796..29929
                     /gene="norW"
                     /locus_tag="N7C74_001206"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HGU2431108.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLLLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKMVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(30057..32309)
                     /gene="hypF"
                     /locus_tag="N7C74_001207"
     CDS             complement(30057..32309)
                     /gene="hypF"
                     /locus_tag="N7C74_001207"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HGU2431109.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPYLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALEDLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQVVLSQHLGDLSDDGIQTQWREALRLMQNIYNFTPQYVV
                     HDAHPGYVSCQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(32462..32989)
                     /gene="hydN"
                     /locus_tag="N7C74_001208"
     CDS             complement(32462..32989)
                     /gene="hydN"
                     /locus_tag="N7C74_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HGU2431110.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(33138..34148)
                     /gene="ascG"
                     /locus_tag="N7C74_001209"
     CDS             complement(33138..34148)
                     /gene="ascG"
                     /locus_tag="N7C74_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HGU2431111.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHKRGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
BASE COUNT         7997 a         9340 c         8739 g         8169 t
ORIGIN      
        1 caaggacttt caaaagaaca aattctggaa aaaaccgggc tatcgcttgg cgtaaaagac
       61 gccagtctgg ccattgaaga aggcgagata tttgtcatca tgggattatc cggctcgggt
      121 aaatccacaa tggtacgcct tctcaatcgc ctgattgaac ccacccgcgg gcaagtactg
      181 attgatggtg tggatattgc caaaatatcc gacgccgaac tccgtgaggt gcgcagaaaa
      241 aagattgcga tggtcttcca gtcctttgcc ttaatgccgc atatgaccgt gctggacaat
      301 actgcgtttg gtatggaatt ggccggaatt aatgccgaag aacgccggga aaaagccctt
      361 gatgcactgc gtcaggtcgg gctggaaaat tatgcccaca gctacccgaa tgaactctct
      421 ggcgggatgc gtcaacgtgt gggattagcc cgcgcgttag cgattaatcc ggatatttta
      481 ttaatggatg aagccttctc ggcgctcgat ccattaattc gcaccgagat gcaggatgag
      541 ctggtaaaat tacaggcgaa acatcagcgc accattgtct ttatttccca cgatctcgat
      601 gaagccatgc gtattggcga ccgaattgcc attatgcaaa atggtgaagt ggtacaggtc
      661 ggcacaccgg atgaaattat caataatccg gcgaatgatt atgtccgtac cttcttccgt
      721 ggcgttgata ttagtcaggt attcagtgcg aaagatattg cccgccggac accgaatggc
      781 ttaattcgta aaacccctgg cttcggccca cgttcggcac tgaaattatt gcaggatgaa
      841 gatcgtgaat atggctacgt tatcgaacgc ggtaataagt ttgtcggcgc agtctccatc
      901 gattcgctta aaaccgcgtt aacgcagcag caaggtcttg atgcggcgct gattgatgcg
      961 ccgttagcag tcgatgcaca aacgcctctt agcgagttgc tctctcatgt cggacaggct
     1021 ccctgtgcgg tgcccgtggt cgacgaggac caacagtatg tcggcatcat ttcgaaagga
     1081 atgctgctgc gcgctttaga tcgtgagggg gtaaataatg gctgatcaaa ataatccgtg
     1141 ggataccacg ccagcggcgg acagtgccgc gcaatccgca gacgcctggg gtacaccggc
     1201 gactgcaccg actgacggcg gtggtgctga ctggctgacc agtacgcctg cgccaaacgt
     1261 cgagcatttt aatattctcg atccgttcca taaaacgctg atcccgctcg acagttgggt
     1321 cactgaaggg atcgactggg tcgttaccca tttccgtccc gtcttccagg gcgtgcgcgt
     1381 tccggttgat tatatcctca acggtttcca gcaattgctg ctgggtatgc ccgcaccggt
     1441 ggcgattatc gttttcgctc tcatcgcctg gcagatttcc ggggtcggaa tgggcgtggc
     1501 gacgctggtt tcgctgattg ccatcggcgc aatcggtgcc tggtcgcagg caatggttac
     1561 tctggcgctg gtgttaaccg ccctgctgtt ctgtatcgtc atcggtttgc cgttggggat
     1621 ctggctggcg agaagtccgc gagcggcgaa aattattcgt ccactgcttg atgccatgca
     1681 gaccacgcca gcgtttgttt atctggtgcc aatcgtcatg ctatttggta tcggtaacgt
     1741 gccgggcgtg gtggtgacga tcatctttgc tctgccgccg attatccgtc tgaccattct
     1801 ggggattaac caggttccgg cggatctgat tgaagcctcg cgctcattcg gtgccagccc
     1861 gcgccagatg ctgttcaaag ttcagttacc gctggcgatg ccgaccatta tggcgggcgt
     1921 taaccagacg ctgatgctgg ccctttctat ggtggtcatc gcctcgatga ttgccgtcgg
     1981 cgggttgggt cagatggtac ttcgtggtat cggtcgtctg gatatggggc ttgccaccgt
     2041 tggcggcgtc gggattgtga tcctcgccat tatcctcgat cgtctgacgc aggccgttgg
     2101 gcgcgactca cgcagtcgcg gcaaccgtcg ctggtacacc actggccctg tcggtctgct
     2161 gacccgccca ttcattaagt aactctgcac ttgcccggtg acgccgggca tatcaccctg
     2221 ccaaaaaaag gaataacaat gcgacatagc gtactttttg cgacagcgtt tgccacgctt
     2281 atctctacac aaacttttgc tgccgacctg ccgggcaaag gcattactgt taatccggtt
     2341 cagagcacca tcaccgaaga aactttccag acgctgctgg tcagccgcgc gctggagaaa
     2401 ttaggttata ccgtcaataa acccagcgaa gtggattaca acgttggcta cacctcgctt
     2461 gcttccggcg atgcaacctt caccgccgtg aactggacgc cactgcatga caacatgtac
     2521 gaagctgccg gtggcgataa gaaattttat cgtgaagggg tatttgttaa cggcgcggca
     2581 cagggttacc tgatcgataa gaaaaccgcc gaccagtaca aaatcaccaa catcgcacaa
     2641 ctgaaagatc cgaagatcgc caaactgttc gataccaacg gcgacggaaa agcggattta
     2701 accggttgta accctggctg gggctgcgaa ggtgcgatca accaccagct tgccgcgtat
     2761 gaactgacca ataccgtgac gcataatcag gggaactacg cggcgatgat ggccgacacc
     2821 atcagtcgtt acaaagaggg caaaccggtg ttttactaca cctggacgcc gtactgggtg
     2881 agtaatgagc tgaagccagg caaagatgtg gtctggttgc aggtgccgtt ctctgcactg
     2941 ccgggcgata aaaatgccga taccaaactg ccgaatggcg cgaattacgg cttcccggtc
     3001 agcaccatgc atatcgttgc caataaagcc tgggccgaga aaaacccggc agcagcgaaa
     3061 ctgtttgcca ttatgcagtt gccagtggca gatattaacg cccagaacgc cattatgcat
     3121 gacggcaaag cctcagaagg cgatattcag ggccacgttg atggctggat caaagcccac
     3181 cagcaacagt tcgatggctg ggtgaatgag gcgttggcag cgcagaagta atttttattc
     3241 gggcggataa ggcgtttacg ccgcatccgc cgttctgtac acaatgcctg atgcgacgct
     3301 ggcgcgtctt atcatgccta caaaccacac cgcacatttc aacaatctat ctttcatccc
     3361 atattcatca acatccgcta ttattgattt ccagcttaat catcacctga tgaacaaaaa
     3421 taatgaccaa acctaatcat gagcttagcc cggcgctaat cgtgctgatg tctatcgcca
     3481 ccggtctggc ggtcgccagc aactattacg cccagccatt gctcgacacc atcgcgcgta
     3541 acttttccct ttccgccagt tcggcaggct ttattgttac cgccgcgcag ttgggctatg
     3601 ccgccggtct gctgtttctt gttcccctcg gtgatatgtt tgaacgccgc cgcctgattg
     3661 tctcgatgac cttactggca gcgggcggta tgttaatcac tgccagcagt cagtcactgg
     3721 cgatgatgat cctcggtacg gcattaaccg gtttattctc agtcgtggca caaattctgg
     3781 ttccgctggc agcgacgctg gcttcaccgg ataaacgcgg caaagtggtt ggcaccatta
     3841 tgagcggtct gctgttgggg atattgctgg cacgaaccgt tgccgggtta ctggcaaacc
     3901 tcggcggctg gcgcaccgtc ttttgggttg cctcggtgtt aatggctctg atggcgctgg
     3961 cattatggcg tggtctgcca caaatgaaat cagaaaccca cctcaactac ccacagttgt
     4021 tgggttccgt tttcagcatg tttatcagca ataagatcct gcgcacccgc gcgttgctgg
     4081 gctgcctgac ctttgccaac ttcagcattc tctggacctc aatggccttt ttgcttgccg
     4141 ctccaccttt taactacagc gatggtgtaa ttggtctgtt tgggcttgcg ggagctgccg
     4201 gagcgttggg cgctcgtccg gcgggcggtt ttgccgacaa gggcaaatcg caccacacca
     4261 caactttcgg tctgcttctg ctgttacttt catggctggc aatctggttt ggtcacactt
     4321 ccgtactggc gttgattatc ggcattctgg tgctggacct caccgtacag ggcgtgcata
     4381 tcactaacca gacggtaatt tatcgaatac atcctgatgc gcgtaatcgc ctgaccgcag
     4441 gttacatgac cagctacttt attggcggtg ccgccggttc gctaatttca gcctcagcct
     4501 ggcaacatgg cggttgggct ggcgtttgtc tggctggcgc gacgattgcc ctggttaact
     4561 tactggtctg gtggcgaggt tttcatcgtc aggaagccgc aaattaagca atcagcaacc
     4621 gttttcgtgg ctttacactt ataagggtgt taagaagccc atcagtctga taaggttaag
     4681 atattcattc agtctattta taatattaac aatcgttaag cgtacactct atggaaagcc
     4741 ctactccaca gcctgctcct ggttcggcga ccttcatgga aggatgcaaa gacagtttac
     4801 cgattgttat tagttatatc ccggtggcct ttgcgttcgg tctgaatgcg acccgtctgg
     4861 gattctctcc tctcgaaagc gtttttttct cctgcatcat ttatgcaggc gcgagccagt
     4921 tcgtcattac cgcgatgctg gcagcaggga gtagtttgtg ggttgctgca ctgaccgtca
     4981 tggcaatgga tgttcgccat gttttgtatg gcccgtcact gcgtagccgt attattcagc
     5041 gtctgcaaaa atcgaaaacc gccctatggg cgtttggcct gacggatgag gtttttgccg
     5101 ccgccaccgc aaaactggta cgcaataatc gccgctggag cgagaactgg atgatcggca
     5161 ttgccttcag ttcatggtca tcgtgggtat ttggtacggt aataggggca ttctccggca
     5221 gcggcttgct gcaaggttat cccgccgttg aagctgcatt aggttttatg cttccggcac
     5281 tctttatgag tttcctgctc gcctcttttc agcgcaaaca atctctttgc gttaccgcag
     5341 cgttagttgg tgcccttgca ggcgtaacgc tattttctat tcccgtcgcc attctggcag
     5401 gcattgtctg tggctgcctc actgcgttaa tccaggcatt ctggcaagga gcgcccgatg
     5461 agctatgagg ttctgctgct tgggttactg gttggcgcgg cgaattattg cttccgctat
     5521 ttgccgctgc gcctgcgtgt gggtaatgcc cgcccaacca aacgtggcgc ggtaggtatt
     5581 ttgctcgaca ccattggcat cgcctcgata tgcgctctgc tggttgtctc taccgcacca
     5641 gaagtgatgc acgatacacg ccgtttcgtg cccacgctgg tcggcttcgc ggtactgggt
     5701 gccagtttct ataaaacacg cagcattatc atcccaacac tgcttagtgc gctggcctat
     5761 gggctcgcct ggaaagtgat ggcgattata taacccacaa gaatcatttt tctaaaacaa
     5821 tacatttact ttatttgtca ctgtcgttac tatatcggct gaaattaatg aggtcatacc
     5881 caaatggata gttcgtttac gcccattgaa caaatgctaa aatttcgcgc cagccgccac
     5941 gaagattttc cttatcagga gatccttctg actcgtcttt gcatgcatat gcaaagcaag
     6001 ctgctggaga accgcaataa aatgctgaag gctcagggga ttaacgagac gttgtttatg
     6061 gcgttgatta cgctggagtc tcaggaaaac cacagtattc agccttctga attaagttgt
     6121 gctcttggat catcccgtac caacgcgacg cgtattgccg atgaactgga aaaacgcggt
     6181 tggatcgaac gtcgtgaaag cgataacgat cgccgctgcc tgcatctgca attaacggaa
     6241 aaaggtcacg agtttttgcg cgaggtttta ccaccgcagc ataactgcct gcatcaactc
     6301 tggtccgcgc tcagcacaac agaaaaagac catctcgagc aaatcacccg caaattgctc
     6361 tcccgtctcg accagatgga acaagacggt gtggttctcg aagcgatgag ctaacgcgtc
     6421 atctcgctca aaaatccaga tttataaaag aaaaaatgac cggccagcat cgcaacatgc
     6481 tggccttttt ggcaagcagg tcggctcagc cgatgagtta agaagatcgt ggagaacaat
     6541 atgagcgcaa atgcggagac tcaaaccccg cagcaaccgg taaagaagag cggcaaacgt
     6601 aagcgtctgc tcctccttct caccttgctc tttataatta ttgccgtagc gatagggatt
     6661 tattggtttt tggtactgcg tcacttcgaa gaaaccgatg acgcatacgt ggcagggaat
     6721 caaattcaaa ttatgtctca ggtgtctggc agcgtgacga aagtctgggc cgataacacc
     6781 gattttgtaa aagaaggcga cgtgctggtc actctcgacc cgacagatgc tcgccaggcg
     6841 tttgaaaaag ccaaaactgc actggcttcc agcgttcgcc aaacccacca gcagatgatt
     6901 aacagcaagc agttgcaggc gaatattgag gtgcaaaaaa tcgccctcgc gaaagcacaa
     6961 agcgactaca accgccgtgt gccgctgggc aatgccaacc tgattggtcg cgaagagctg
     7021 caacacgccc gcgacgccgt taccagtgcc caggcgcaac tggacgtcgc gattcaacaa
     7081 tacaatgcca atcaggcgat gattctgggg actaaactgg aagatcagcc tgccgtgcaa
     7141 caggctgcca ccgaagtacg taacgcctgg ctggcgctgg agcgtactcg tattgtcagt
     7201 ccgatgaccg gttatgtctc ccgccgcgcg gtacagcctg gggcgcaaat tagcccaacg
     7261 acgccgctga tggcggtcgt tccagccacc aatatgtggg tggatgccaa ctttaaagag
     7321 acgcagattg ccaatatgcg tatcggtcag ccggtcacta tcaccaccga tatttacggc
     7381 gatgatgtga aatacaccgg taaagtggtt ggtctggata tgggcacagg tagcgcgttc
     7441 tcactgcttc cggcgcaaaa tgcgaccggt aactggatca aagtcgttca gcgtctgcct
     7501 gtgcgtatcg aactggacca gaaacagctg gagcaatacc cgctgcgtat cggtttatcc
     7561 acgctggtga gcgtcaatac cactaaccgt gacggtcagg tactggcaaa taaagtacgt
     7621 tccactccgg tagcggtaag caccgcgcgt gaaatcagcc tggcacctgt caataaactg
     7681 atcgacgata tcgtaaaagc taacgctggc taatccagag gtgcgtgtga tgcaacagca
     7741 aaaaccgctg gaaggcgcgc aactggtcat tatgacgatt gcgctgtcac tagcgacatt
     7801 catgcaggtg ctggactcca ccattgctaa cgtggcgatc cccactatcg ccgggaatct
     7861 tggctcatcg ctcagccagg gaacgtgggt aatcacttct ttcggggtgg cgaatgccat
     7921 ctcgatcccg cttaccggct ggctggcaaa gcgcgtcggg gaagtgaaac tgttcctctg
     7981 gtcaaccatc gcctttgcta ttgcgtcgtg ggcgtgtggt gtctccagca gcctgaatat
     8041 gctgatcttc ttccgcgtga ttcaggggat tgtcgccggg ccgttgatcc cgctttcgca
     8101 aagtctattg ctgaataatt acccgccagc caaacgctca atcgcgctgg cgttgtggtc
     8161 gatgacggtg attgtcgcgc caatttgcgg cccaatcctc ggcggatata tcagcgataa
     8221 ttaccactgg ggctggatat tcttcatcaa cgtgccgatt ggcgtggcgg tggtgttgat
     8281 gacactgcaa actctgcgcg gacgtgaaac ccgcaccgaa cggcggcgga ttgatgccgt
     8341 ggggctggca ctgctggtta ttggtatcgg cagcctgcag attatgctcg accgcggtaa
     8401 agagctggac tggttttcat cacaggaaat tatcatcctt accgtggtgg cggtggtggc
     8461 tatctgcttc ctgatcgtct gggagctgac cgacgataac ccgatagtcg atctgtcgtt
     8521 gtttaagtcg cgcaacttca ccatcggctg tttgtgtatc agcctcgcgt atatgctcta
     8581 cttcggcgcg attgttctgc tgccgcagtt gttgcaggag gtctacggtt acacggcgac
     8641 ctgggcaggt ttggcctctg cgccggtagg gattattcca gtgatcctgt cgccgattat
     8701 cggccgcttc gcgcataaac tggatatgcg ccgactggta accttcagct ttattatgta
     8761 tgccgtctgc ttctactggc gtgcctatac ctttgaacca ggtatggatt ttggcgcgtc
     8821 ggcctggccg cagtttatcc aggggtttgc ggtggcctgc ttctttatgc cgctgaccac
     8881 cattacgctg tctggtttgc caccggaacg actggcggcg gcatcgagcc tctccaactt
     8941 tacgcgtacg ctggcggggt ctatcggcac gtcgataacc acaaccatgt ggaccaaccg
     9001 cgagtcgatg caccatgcgc agttgactga gtcggtaaac ccgttcaacc cgaatgccca
     9061 ggcgatgtac agtcaactgg aagggcttgg gatgacacaa cagcaggcat caggctggat
     9121 tgcccagcag atcaccaatc aggggctgat tatttccgcc aatgagatct tctggatgtc
     9181 ggccgggata ttcctcgtct tgctggggct ggtgtggttt gctaaaccgc cctttggcgc
     9241 aggtggcggc ggaggcggtg cgcactaagt acaactaagc cagttcattt gaactggctt
     9301 ttttcaatta attgtgaaga tagtttactg actaaatgtg cagttcctgc aacttctctt
     9361 tcggcagcgc cagttcttcg ttgctgttga tgcgcacgtc acgttccaga atgctacgcg
     9421 caatatcctg cgcttcctgc aacgagtgca tctggtaagt gccacactgg tagacgttca
     9481 gctccggaat ctgattctga tcctgcactt tcagcacgtc ttccattgcc gctttccaga
     9541 catcagcaac acgctgctca tctggcgtac caatcagact catataaaaa ccggtgcggc
     9601 agcccattgg cgagatatcg ataatctcta caccattacc gttaagatgg ttacgcataa
     9661 aaccagcaaa caggtgctcc agggtatgga tccctctttc tggcatcact tctttgttcg
     9721 gcacgcagaa gcgcagatcg aacacggtga ttgcgtcgcc atgcggggtg ttcattgttt
     9781 tcgccacccg aactgcaggc gcttccatcc gggtatgatc gactgtgaag ctatctaaca
     9841 acggcattta gccacctccg gtaatttttt taaaaatttt ctgaactctt tcttcccagg
     9901 cgagtctgag tatatgaaag acgcgcattt gttatcatca tccctgaatt cagagatgaa
     9961 attttggcca ctcatgagtg gcctttttct tttctgtcag gcgtgttttt ccagccacac
    10021 cgcaaacggt tcggtatctg cggcttccat ttcctgctga cggcgttcag atgcctcgcg
    10081 ctcggctaca aaatcctctt cgcgcagaat ttccagcggc tcttcacgca gcagattacg
    10141 gtacgcttca gcaaacgctt tgcctgttcc gccaatacca gtatcaatca tagaccttaa
    10201 gatacgggca gagaaagtca gatcgggatt atcgaagcag gcaaccagtt catcacacac
    10261 tttctgatac gcttcgccgc cgttaatgct atccagcgtt tgcgcgacgc gtttcagatc
    10321 gcggaacaga tctttaccca cctgcggtaa cgggaactgt gcggtttcgc agccgatacc
    10381 cagcgtcaga cccggtttgc gaccttcgag gatcacccgg ttccagttaa cgcgtgtaca
    10441 ggcaagttcg ctactgctca tttccggtgc atcagccagc gcacaccaga ccataaacag
    10501 gtcgaggaat cgcacctgct gttcatctac accaatcggc gagaacgggt tgatgtccag
    10561 cgaacgcact tcaatatatt caatgccgcc acgtaacagc gcatcagaag gcgactcgcc
    10621 gctgcgggta acgcgttttg gacgaatcgg cgcgtacagt tcgttttcaa tctgcagcac
    10681 gttgctgttg atttgcagcc tcttaccgtc tttatcaata ccaatcttcg cgtactcttc
    10741 cgatggcgtt ttgattgcct gtttcaggcc cgctacgtac tcgtaaagat cgttgaaggt
    10801 aataccaaga ttgctttgcg atttattggt atagccgaga tcgctcaaac gaagagaggt
    10861 cgcatacggc aggtaataca taccgcactc ggttttctca aacggcagcg acgttggttt
    10921 accttgcagg aatgaagagc atattgccgg agacgcgcca aacagataag gaatgaccca
    10981 accgaagcga tagtagttgc gaataacgcg gaaatagccc gcagagattt tctctttagc
    11041 atcagcgccc gaaatatcgc cacacttcgc ttgccagaat gccataggca aagagaagtt
    11101 gtaatgcacg ccggagatgg tttgcatcag cgcgccgtag cgatttttca gcccttcacg
    11161 gtacagcgtt ttaaagcgtc cggtgttaga agtgccgtac tgcgccagtt cgatgtcctg
    11221 accttctgcg atgtagcatg gcatacttaa cggccacatc cgctcatcgc ccatattgcg
    11281 cgccgtataa cgatgcagat cgcgcataaa ggtcagcata tgttcaatat caccatccac
    11341 tggtgtaatg aattccagca atgcttccgc aaaatcggta gtaatccatt tatgcgtcag
    11401 tgcggaacct aatgcttcag gatgacctgt tgttgccagt gtgccatcag cattaacacg
    11461 caaagtttcg cgctccagcc cacgctgtat cccctttaac gcctgaggat gtttttccag
    11521 ccaggccagc gcctgtgata cgtccgggat caaattgacc tcccgcctgt caaaatcgtt
    11581 ttaattagca taactgtaat ggtgaccata tgtgcaggcc tacaattagt gccaccacat
    11641 catgccctga acggtcgctg ctgcaactgc aacatagcgt aacgctttac caaggcacaa
    11701 aaaaaagatt accggccccc aggagatgcg catccatccc gctaacagac acagtaaatc
    11761 gccaaccacg ggcatccagc ttaataatag cgtgactgca ccatagcgtt tcagccagcc
    11821 ggtagctttc tcttgccagc gcgatgtttt acgcaatgga cagaaacgcc caaggataac
    11881 gttagttaac cctccaaggc tattacccat tgttgctgtt aaaactaaaa cccagggatg
    11941 actgatccca gaaagcaaca ttgccaccag cacgacttcg gagttgccgg gtaatagtgt
    12001 agcgctgaga aaactactgg caaacaacga gaaaagcgat aacgcttcac tcacagcaag
    12061 cgaacatcca cagcgtccat gcctgctgca cgggccgcct gaataccgaa atcggcatct
    12121 tcaaagacca cacactgcgt cggttgcacg cccatacgct gcgcgcacaa caaaaatgtg
    12181 tctggcgcgg gtttatggtg tttgacgtga tcggcagcga cgacggcgtc aaaataacgg
    12241 cgtaatccca ggtgagccag caatgcctca gcgatggcgc tttcactccc cgttcctaca
    12301 gccattgggc gacgaccatg ccaacttttc accacttcaa caagaggaag cggctcgaca
    12361 ctatccagca gcatacttct tactgcttct gttttttcac gcgctaacgc atgcgggtcg
    12421 agatcggcct gattcagctc aataattgcc tgagcaatac gccaggtggg cgatccatta
    12481 agcgcaatca tcgcctgaac atcgtactga agaccgtagt gccctaatac ttcgcgccac
    12541 gctttacggt gcgtaggctc cgtatccagg attgtgccat ccatatcaaa aattaaacct
    12601 gcataacgct cgtacatggt cttctcgcaa gtcgaaaaat atgacgctac tttagcgtaa
    12661 ttgcttgatt ttgtcgctga tggcgaagga gaagaaaatt caggaaatgg ataaagtagt
    12721 tatttagaaa tgagatattt ttgaaggaaa ttttttggag aagatggtgc atccgggagg
    12781 atgactcact ggcgttcgcc cttcgggccg ttgctaaagc aacgttatcc ttcctggtgc
    12841 ctgcggttaa ttccgctacc tcttcgatat cttcattgct gaagatttcg gagaatatgg
    12901 tgcatccggg aggattcgaa cctccgaccg ctcggttcgt agccgagtac tctatccagc
    12961 tgagctacgg atgcatcggg aaaatttatt ttactgctga tattgatatc gctactaaag
    13021 ccatatcaaa taagaaaatc catcttaacc atcatcactg atgattacgg agaatatggt
    13081 gcatccggga ggattcgaac ctccgaccgc tcggttcgta gccgagtact ctatccagct
    13141 gagctacgga tgcatcggga aatttatttt actgctgata ttgataccgc tactaaagct
    13201 gtatcaagta agagatggtg catccgggag gattcgaacc tccgaccgct cggttcgtag
    13261 ccgagtactc tatccagctg agctacggat gcatcgggaa acttacttta ctgcagattt
    13321 tttgataccg ctactaaagc cgtatcaagt aagagatggt gcatccggga ggattcgaac
    13381 ctccgaccgc tcggttcgta gccgagtact ctatccagct gagctacgga tgcaaatggc
    13441 ggtgaggcgg ggattcgaac cccggatgca gcttttgacc gcatactccc ttagcagggg
    13501 agcgccttca gcctctcggc cacctcacca cacgcctctt acgagtgctt cgaagaactt
    13561 gtttatcgct catcgtcgct gcgtggcgca catattactt tctgagactt ataagtcaaa
    13621 caatttttcc cacactttta tcgtttgcac acttcacgct caattagtct gtaaaaacgt
    13681 caaaaagagt gttttatcta cagaaaaacg gaggtctggc agatagcagt aatgcaaaaa
    13741 aatggagact taagttgaat gaacgggagt aaagcgaaaa gactatagag tgaaggagaa
    13801 attttgaggg tgcgtctcac cgataaagat gagacgcgga aagattagta actggactgc
    13861 tgggattttt cagcctggat acgctggtag atctcttcac ggtgaacaga aacttccttc
    13921 ggggcattta cgccaatacg tacctggttg ccctttaccc ctaaaactgt cacggtgacc
    13981 tcatccccaa tcatgagggt ctcaccaact cgacgagtca gaatcagcat tctttgctcc
    14041 ttgaaagatt aaaagagtcg ggtctctctg tatcccggca ttatccatca tataacgcca
    14101 aaaagtaagc aatgacaaac acattacatg taagcagtca tggcattaca ttctgttaaa
    14161 cctaagttta gccgatatac acaacttcaa cctgacttta tcgttgtcga tagcgttaat
    14221 gcgaatgccg tgaagcgagt ccacggcatt gcctgacgct tatattattg caatttcgcg
    14281 ctgacccagc ctttcacact ggctaacgct gcaggtaagg ccgcagcatc cgtaccaccg
    14341 gcttgcgcca tgtcaggacg tccaccaccc ttgccgccca cctgctgagc gaccatacca
    14401 atcagttccc ctgctttcac acgatctgtg acgtccttag atacgcctgc aatcagagaa
    14461 accttacctt cggctaccgt tgccagcacg ataattgtcg accccagctg attttttaaa
    14521 tcgtcaacca tggtacgcaa cattttcggc tcaacaccgc taagctcgct aaccaacagc
    14581 ttaacaccat taacatcaat tgccttactg gaaagatttg cgctctcctg tgcggcagct
    14641 tgttctttaa gctgttgtag ttctttttct aactgacgcg tacgttccag tactgagcgc
    14701 actttatccg ccagattatt gctatcgcct ttcagcagat gcgcgacttc gcttaagcga
    14761 tcgctgtctg catgaacggt ggtgatagcg ccttctccgg ttaccgcttc gatacgacga
    14821 acgcctgcag cagtacccga ttcagagatg atgcggaaca gaccaatatc accagtgcgg
    14881 ctggcgtgag taccgccaca cagctcggtg gagaaatcgc ccatgctcag cacgcgtaca
    14941 cgctcatcat acttctcgcc gaacagcgcc atcgcacctt tcgctttcgc cgcttcgaga
    15001 tccatgatgt tggtttcgat cggcaaattg cgacgaatct gtgcgttcac caggtcttcg
    15061 accgcacgaa tctcttccgg tttcatcgct tcgttgtgtg agaagtcgaa gcgcagcacc
    15121 ttgtcgttaa ccagtgaacc tttctgcgat acatgagtcc ccagaacctg gcgcagcgca
    15181 gcgtgcatca ggtgcgttgc ggagtgattc aaacgaatac gggcgcgacg agcctcatca
    15241 acatcagcct gcacagcgtc gcccactttc agagaacccg cagcaagttt accgatgtga
    15301 ccaatcgcct ggccgtattt ctgcgtatct tccaccacga aggagaagtt agcgcctttc
    15361 agttcgcctt tatcaccaac ctgaccgccg gattccgcat agaatggcgt ttgatccagc
    15421 acgaccacag cttcctggcc tgcattgatg gcatcaaccg ctttaccatc aacaaacagc
    15481 gcggtcactt tgccgttcag ttccagatgg tcatagcctt taaattcaga tgcactgtca
    15541 acacggatca ttgcgttgta atcggcacca aagccgctgg cttcgcgcgc ccgacgacgc
    15601 tgctcttcca ttgctgcttc aaaaccagct tcgtcaactt tgatgttgcg ctcacgacaa
    15661 acatcagccg tcaggtcaac cgggaagcca taggtgtcgt acagacggaa agcagtttca
    15721 ccatccagcg tatcaccaga aagttttgcc agctcttcat ccagcaacgc cagaccgcgc
    15781 tccagagtac gagcaaactg ctcttcttca gtcttcagca cctgctcaac ctgcgcctgc
    15841 tggcgtttca ggtcttcacc cgcagagccc ataacgtcga tcagcggacc aaccagtttg
    15901 tagaagaagg tttctttcgc gccgagcata ttaccgtgac gcactgcgcg acgaatgata
    15961 cgacgcagta cataaccacg gttttcattg gacggcatta cgccatccgc gatcaggaac
    16021 gcacaagaac gaatgtggtc agcgattacg cgcagcgatt tattgctcag atcggttgcg
    16081 ccagtgactt tcgctaccgc ctggatcaac gtgcggaaca ggtcgatgtc atagttagag
    16141 ttaacgtgtt gcagcaccgc agcaatacgc tccagaccca taccggtatc tacagacggc
    16201 ttcggcagcg gttccatcgt gccatcggcc tggcggttga actgcatgaa gacgatgttc
    16261 cagatctcaa tgtagcggtc gccgtcttct tccggacttc ccggagggcc accccaaatg
    16321 tggtcgccgt gatcgtagaa gatttcggtg cacgggccgc acggaccagt gtcacccatc
    16381 tgccagaagt tgtcagatgc gtatggcgca cctttgttat cgccgatgcg aataatacgt
    16441 tcgcgcggga tccctacttc tttttcccag atttcgtagg cttcgtcgtc gctttcatag
    16501 acggtaaccc acagacgctc tttcggcagg gcaaaccatt tttcgctggt cagcagttcc
    16561 catgcaaact gaatggcatc gtgtttgaaa tagtcgccga agctgaagtt gcccagcatt
    16621 tcgaagaagg tatggtgacg cgcggtgtaa ccgacgtttt ccaggtcgtt gtgtttacca
    16681 cccgcacgca cgcagcgttg ggaagtggta gcgcgggaat aattacgctt gtcgagccca
    16741 aggaacacat ccttgaactg gttcatcccg gcgttggtaa acaacaaagt tgggtcgtta
    16801 tggggtacca gggagctgct ggcaactacc tgatgtccct tactatggaa aaagtcgaga
    16861 aacgcctgac ggatctcagc ggtgctcttg ctcataatta tcctgaaatc aagctaacga
    16921 aatatcgcca ccagctccag cgtgtcttaa ccgccgggct ggtaactgaa aagtgggaat
    16981 aagataagtt ttcttgactg ggaagtaaaa taccgtatgc gttcagtcgg caaaatttcg
    17041 ccaaatatcc tggatatctt ccatcagata gccacgatag agcagaaaac gctggatctt
    17101 aactttttct gaaaagacag ttggcaaagg ttcaccatat tttcgcgtcg cctgatcgcg
    17161 cgccagtgcg caccagtcga tgtcacattc acgcatcgct ttttctgtcg cttcgcggga
    17221 aatacctttc tgattcagtt cctggcgaat acgcgcaggt ccataacctt tgcggctacg
    17281 gctggcgata aagcgcgcaa caaatcggct gtcatcgaga tagccatgtt catggcacca
    17341 ggcaataacg cgctcgtaat cttctgccgt agcatcaatc tcttctgggc catttttgcc
    17401 cataatcggt gccgcgagtt tacgtcgcag ttcttgctca ctgtgatcgc gcaccgccag
    17461 aatgcgtacc gcacgatcca acaggcgagc atatgccggg cgacgggacg ttgattctgt
    17521 catggcatat ccttacaact taaaaagcaa aagggccgca gatgcgaccc ttgtgtatca
    17581 aacaagacga ttaaaaatct tcgttagttt ctgctacgcc ttcgctatca tctacagaga
    17641 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
    17701 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
    17761 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
    17821 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctgg aattcagcct
    17881 gtttaaacgg cgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
    17941 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
    18001 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
    18061 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
    18121 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
    18181 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
    18241 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggtg tccggctggg
    18301 agcacagcag gttgtcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
    18361 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggca atcacctgca
    18421 acgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
    18481 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
    18541 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
    18601 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
    18661 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaagcaatt atactgtatg
    18721 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
    18781 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgcag ggtaatgcct
    18841 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
    18901 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
    18961 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
    19021 tcttcgcgta cgccgatcat ttgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
    19081 tcaaaccagg cggaactgcc ggcaatatcg gtaatcactt tcgctaccca accgccggta
    19141 caagactcag cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
    19201 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
    19261 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
    19321 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgcaaa tagttactgc
    19381 acgcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtactg
    19441 tggttataac gggtaatggt gtagaagttc ggcagaccgt accagtactg gtaacctgtt
    19501 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
    19561 aaacctgccg cggcaagctg cgaaatgctg tacctggttt tgaagccatt tggcaagcct
    19621 ggagcctgac cgtttgccat taccgcgacc tgatcgcctt tcacccagcc gtgcgctttg
    19681 aaataattcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
    19741 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
    19801 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
    19861 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
    19921 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
    19981 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
    20041 gcatcttcat actgattcca gaaaaccaca ccgttctgca cattgtccgg cgtaataaat
    20101 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
    20161 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gtttcgcctg ggagagaatt
    20221 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttctgtcgat gaactgctgg
    20281 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
    20341 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
    20401 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
    20461 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac gccccgcaca
    20521 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt ctcctcgcta
    20581 tccttttact caaactttca aattaaaata tttatctttc attttgcgat caaaataaca
    20641 ctttaaatct ttcaatctga ttagattagg ttgccgtttg gtaataaaac aataaatcct
    20701 gaaggagaga acaatgatag aaaccattac tcatggtgca gagtggttta tcgggctgtt
    20761 ccaaaagggc ggagaggtgt ttaccgggat ggtgaccggc attcttccgc tgttgattag
    20821 cctgctggtt atcatgaacg cactgattaa ttttatcggt cagcatcgta ttgaacgttt
    20881 tgctcaacgt tgcgccggta accctgtttc ccgttaccta ctgttaccgt gcattggcac
    20941 gtttgtcttt tgcaatccga tgaccttaag cctgggtcgc tttatgccgg aaaagtacaa
    21001 acccagctac tacgcggcgg cctcttatag ctgccactca atgaatggcc tcttccccca
    21061 tatcaaccct ggcgaactgt ttgttatctt ggcattgcca gcggtctgac aacgctgaac
    21121 ctgccacttg gcccactggc ggtgagctat ctgctggttg gtctggtcac caacttcttc
    21181 cgcggctggg tgaccgatct gaccaccgct atttttgaga aaaagatggg cattcaactt
    21241 gaacaaaaag ttcatctggc aggagcaaca tcatgacgca tattcggatc gaaaaaggaa
    21301 cggggggctg gggcggcccg cttgagctgg aaaccacgcc gggcaaaaaa atcgtctata
    21361 tcaccccgcc ggtacccggc ctgcgattgt cgacaaactg gcacagctta ctggctggca
    21421 ggctattgac ggatttaaag aaggtgaacc cgcggaggcg gaaattggtg tcgcggtaat
    21481 cgactgtggc ggcacattac gctgcggcat ttatccgaaa cggcgtattc ccaccattaa
    21541 tatccactcg acgggcaagt ccggtccact ggcgcagtac attgtggaag atatttatgt
    21601 ctctggcgta aaagaagaaa acatcactgt agtgggtgat gcgacaccac aaccctcttc
    21661 cgtgggccgt gactatgaca ccagcaagaa aatcaccgaa caaagcgatg gtttactggc
    21721 gaaggtggga atgggtatgg gttctgccgt tgccgtgttg tttcaatctg gtcgtgacac
    21781 catcgacact gtattaaaaa ccattcttcc gtttatggca ttcgtctcgg cgctcattgg
    21841 catcattatg gcttctggcc ttggtgactg gattgcccac ggccttgctc cgctggcgag
    21901 ccatccactg ggtctggtca tgctggcgct catctgctcc ttcccgctgc tttcaccttt
    21961 cctcggccca ggcgcagtta tcgcacaggt tatcggcgta ttgattggcg tgcagattgg
    22021 tctcggcaat attccgccgc atctggcttt acctgcactg tttgccatca acgcgcaggc
    22081 ggcctgcgac ttcattccgg tcggtttgtc gctggcggaa gcccgtcagg acacggttcg
    22141 cgtcggtgtc ccttctgtac tggtgagccg ctttttaacc ggcgcgccga ctgtactgat
    22201 cgcctggttt gtctccggtt ttatctatca atagaggctg aaacatgacc gttatttatc
    22261 agaccaccat cacccgtatc ggcgcgagtg ccactgacgc cctcagcgac cagatgctca
    22321 tcacctttcg tgaaggcgcg ccagcggacc tcgaagagta ttgcttcatt cattgccacg
    22381 gcgagttgaa aggtgcactc catcccggtt tgcaatttac gctcgggcag catcgctatc
    22441 cggtgaccgc tgttggcagc gtggcggaag acaaccttcg cgaactgggt catgtcaccc
    22501 tgcgcttcga tggtttaaac gaagcggaat ttccgggcac tgtccatgtg gcaggccctg
    22561 tacccgacga tatcgcgccg ggatcggttt tgaagtttga atctgttaag gagtaaaaaa
    22621 tgaatcaggt tgccgttgtc atcggtggtg ggcaaacctt aggcgcgttc ctgtgccacg
    22681 gtctggctgc cgaggggtat cgcgtcgcgg ttgtcgatat tcagagcgac aaagccgcaa
    22741 atgtggcaca agaaattaac gccgaatatg gtgaaggaac agcgtatggt tttggtgctg
    22801 acgccactag cgagcaaagc gttctggcgc tctctcgtgg ggtagatgaa atctttgttc
    22861 gtgtggattt actggtctac agcgccggaa ttgcaaaagc agcctttatc agcgacttcc
    22921 agctcggcga ttttgaccgt tcgctacagg tgaatctggt gggttatttc ctgtgtgcgc
    22981 gtgaattttc gcgtttgatg atccgcgatg ggattcaggg acgcattatt cagatcaact
    23041 cgaaatccgg caaagtgggc agcaaacaca attctggtta tagcgccgcg aaatttggtg
    23101 gcgtagggct gactcaatca ctggcgctgg atctggcgga gtacggcatt acggtgcatt
    23161 cactgatgct cggtaacctg ctgaaatcgc cgatgttcca gtcgctgttg ccacaatatg
    23221 ccaccaagct gggtatcaaa ccggatcaag tcgagcagta ttacatcgac aaagtaccgc
    23281 tcaaacgcgg ctgcgattat caggatgtgc tgaatatgct gctgttctac gccagtccta
    23341 aggcgtcgta ctgtaccgga cagtcgatca atgtcaccgg cggtcaggtg atgttctgat
    23401 caacagcgga gatccgttaa ggatctccgt gagactaata gaatgcctga tgcgctacgc
    23461 tcatcaggca tacaggactt ccgccactac attaaggaaa agttatggta tccgcactca
    23521 tcactgtcgc cgttatcgcc tggtgtgcgc aactggcctt aggcggctgg caaatttctc
    23581 gttttaaccg tgccttcgac acactatgcc agcaagggcg ggttggcgtg ggccgttcca
    23641 gcgggcgctt taaaccgcgg gtcgtggtcg ccatcgccct ggacgatcag cagcgcatcg
    23701 ccgacacctt gtttatgaaa ggactgaccg tcttcgcccg accgcaaaaa attcccgcaa
    23761 ttaccggtat gcatgcgggt gatttacagc ccgatgtgat ctttccccat gatccactat
    23821 cacagaatgc tctatcattg gcgcttaaac tgaaacgtgg ataatttcgt tgtgaatgtt
    23881 acttgcttgc gaagttatca ttttgaaacc taaatcaggt aatcacgccc atgaaacctc
    23941 gtcagcgtca agccgccatt ctggagtatc tgcaaaagca gggaaaatgc tcggttgaag
    24001 aattggcgca atattttgac accacaggca caactattcg caaagatctg gtcattctgg
    24061 aacatgccgg aaccgtcatt cgtacttatg gcggagtggt gttgaataaa gaggaatccg
    24121 atccgcctat cgatcataaa acactcatca acacccacaa gaaagagctg atagcagaag
    24181 ctgccgttgg ttttattcat gatggcgatt cgatcattct tgatgctggc agtaccgttt
    24241 tgcagatggt gcctctgctc tcgcgcttta ataacatcac ggtgatgacc aacagcctgc
    24301 atatcgtcaa tgcgttatct gaactggata atgaacaaac tatcctgatg ccaggcggaa
    24361 cgtttcgtaa aaaatcggcc tcatttcacg ggcagctggc agagaatgcc ttcgagcatt
    24421 tcaccttcga caaattattc atgggtaccg acggcatcga tctcaatgcg ggcgtaacca
    24481 ccttcaacga ggtctatacc gtcagtaagg caatgtgcaa tgccgcgcgc gaagtgattt
    24541 tgatggcgga ctcatcaaag tttggccgta aaagccccaa cgtagtttgc agtcttgaaa
    24601 gcgtcgataa gctgattacc gacgcagata tcgatccggc gtttcgtcag gcgctggaag
    24661 agaaagggat cgatgtgatc ataaccggag agagcaatga gtgaagcact actgaacgcg
    24721 ggacgtcaga cgttaatgct ggaattgcag gaagcaagcc gtttaccgga acgtctgggc
    24781 gatgattttg ttcgcgccgc caatatcatc ctgcactgcg aaggcaaagt ggtggtttcg
    24841 ggaattggca aatcgggcca cattggtaag aaaatcgccg caacgcttgc cagtaccggc
    24901 acaccggctt tttttgtcca tccggcagaa gcgctgcatg gcgatctggg gatgatcgaa
    24961 agccgcgatg tgatgctgtt tatctcttac tccggtgggg cgaaggaact ggatctgatt
    25021 attccgcgtc tggaagacaa atctatcgcg ctgctggcga tgaccggcaa accgacgtca
    25081 ccgctgggcc tggcggcgaa agcggtgctg gatatctccg tagaacgcga agcctgcccg
    25141 atgcaccttg cgccgacctc cagcaccgtc aataccctga tgatgggtga cgcgctggcg
    25201 atggcggtca tgcaggcgcg cggatttaat gaagaagatt ttgcccgctc ccaccctgct
    25261 ggagcactgg gcgctcgctt gctgaataaa gtgcatcatc tgatgcgccg tgacgatgcc
    25321 atcccacagg tggcgttaac cgccagcgtg atggatgcta tgctggaact cagccgcacc
    25381 ggtctggggc tggtggcggt atgtgacgct caacaacagg tacaaggcgt ctttaccgac
    25441 ggcgatttac gtcgctggct ggttggcggc ggcgcactca caacgccagt caatgaagcg
    25501 atgacggtcg gcggcaccac gttgcaatcg caaagtcgcg ccatcgacgc caaagagatc
    25561 ctgatgaagc gcaaaatcac tgccgcaccg gtggtggatg aaaacggcaa actcaccggc
    25621 gcaataaacc tgcaggattt ctatcaggcc gggattattt aatccttcaa tcccagacgt
    25681 ttcgccagcc gatgcaggtt ggcgacgtcg gtttccagca tccgcgcgca ggcagcccag
    25741 ttgtgatgat tttgtgccag tgcctgacga atagtttcac gctggaacgc ttctgtcgct
    25801 tcacgcaggt tttgcttaac aacgggcacc gccgccactt ctggcggcgg caacgtcacc
    25861 tcaggaaaag caaaatgttg cgcctcaaga atcacttcat cgccgctgcg ggtggctctc
    25921 gccagcacta ccgcccgatg aatagcatgt tccagttcgc gcacgtttcc cggaaaactg
    25981 tagtgttgca gtaaatttcg cgccccggca cttaatacca cgcgggagag cccctgccgc
    26041 aaacgacact gctcgcagaa ataccccgcc agcagaatga catcatcgcc ccgctcacgc
    26101 agcggcggca ccgaaagtgg aaacacactc aggcgatgaa acaaatcggc gcggaatcgc
    26161 cctgccagca cctcttcgcg taagtcgcgg ttagtcgccg ccagcacgcg cacatcgacc
    26221 cgcaaactac ggtcatcgcc aacgcgctga atatcgccat actgcaacac cctcagcagc
    26281 ttggcctgca atgccaacga caactcgccg atctcatcga gaaacagcgt gccgttatcc
    26341 gccatttcaa acttcccact gcggttactg atagcgccag taaacgctcc tttcacatgc
    26401 ccgaacaact cactttccgc cacactttcc ggcagtgcgg cacagttgag atagaccagc
    26461 ggattcaccg cccgtggcga ggcctcatga atcgctttcg ccaccagctc cttaccggtt
    26521 cccgtctcac cgctgatcag gacgttgaga tcggacgccg ccacaatctc aatctctttt
    26581 ttcagttgcg tcatgcctgg cgacagaccg atcatctgcg tctgtttcac cgcttcaaat
    26641 ggagcggcat cgcccggcag catattctgg ctttccagtt gttcaatcag caacgcattg
    26701 cttaacgctc ccgccgccag cgcggcaatc aggcgtaact cttcgtcgct gaaaacatcg
    26761 aactgatcgg gctgcatccc gtcgagcgtc aatgcgccga tcaggttttg cccggcaaac
    26821 agcggcagac caacgcaggc gtgaaccttc agactctcct gccctggaat caaaccgtca
    26881 tagggatcgg gcaattcgct gtctgcggga aagcgcacca catcacccgc gcgggcaatc
    26941 gcttccagcc gtggatgacc ttccagcgca aagcgtctac cgagtacatc cttcgccagc
    27001 ccgtcgatgg caagcggaat aaactgccgc gagtcgtaac gtagcaacgc agacgcatcg
    27061 cactccagca cctgacgcag cgtggtgatc aggcgctgaa aacgatcctg atgaccaatc
    27121 ccacgctgca attcgatggc gatattcgcc agcacatcaa cggaaaaact catctttgcc
    27181 tcactgtcaa tttgactata gatattgtca tatcgaccat tggattgata gtcattttga
    27241 ctactcatta atgagcataa ttttatttat agagtaaaaa caatcagata aaaaactggc
    27301 acgcaatctg caattagcaa gacatctttt tagaacacgc tgaataaatt gaggttgcta
    27361 tgtctattgt ggtgaaaaat aacattcatt gggttggtca acgtgactgg gaagtacgag
    27421 attttcacgg cacggaatat aaaacgctgc gcggcagcag ctacaacagc tacctcatcc
    27481 gcgaagaaaa aaacgtgctg atcgacaccg tcgaccataa attcagccgc gaatttgtgc
    27541 agaacctgcg taatgaaatc gatctggcgg atatcgatta catcgtgatt aaccatgcag
    27601 aagaggacca cgccggggcg ctgaccgaac tgatggcaca aattcccgat acgccgatct
    27661 actgtacagc caacgctatc gactcgataa atggtcatca ccatcatccg gagtggaatt
    27721 ttaatgtggt gaaaactggc gacacgctgg atatcggcaa cggcaaacag ctcatttttg
    27781 tcgaaacacc aatgctgcac tggccggaca gcatgatgac ttacctgaca ggcgacgcgg
    27841 tgctgttcag taacgatgct ttcggtcagc actactgcga cgagcatctg ttcaacgatg
    27901 aagtggatca gacggagctt tttgagcagt gccagcgtta ctacgccaat atccttacgc
    27961 cgttcagccg tctggtaacg ccgaaaatta ccgagatcct gggctttaac ttaccagtcg
    28021 atatgatagc gacctctcac ggcgtggtat ggcgcgataa cccgacgcaa attgtcgagc
    28081 tgtacctgaa atgggcggct gattatcagg aagacagaat caccattttc tacgacacca
    28141 tgtcgaataa cacccgcatg atggctgacg ctatcgccca ggggattgcg gaaaccgacc
    28201 cacgcgtggc ggtgaaaatt ttcaacgtcg cccgaagcga taaaaacgaa atcctgacca
    28261 atgtcttccg ctcaaaaggc gtgctggtcg gcacttcgac gatgaataac gtgatgatgc
    28321 cgaaaatcgc cgggctggtg gagaagatga ccggattacg cttccgtaac aaacgcgcca
    28381 gtgctttcgg ctctcacggc tggagcggcg gtgcggtgga tcgtctttcc acgcgcctgc
    28441 aggatgcggg tttcgaaatg tcgcttagcc tgaaagcgaa atggcgacca gaccaggacg
    28501 ctctggagtt atgccgtgaa cacggtcgcg aaatcgcccg tcagtgggcg ctcgcgccgc
    28561 tgccgcagag cacggtgaat acggtagtta aagaagaaac ctctgccacc acgacggctg
    28621 acctcggccc acggatgcag tgcagcgtct gccagtggat ttacgatccg gcaaaaggcg
    28681 agccaatgca ggacgttgcg ccaggaacgc cgtggagtga agtcccggat aacttcctgt
    28741 gcccggaatg ctccctcggc aaagacgtct ttgacgaact ggcatcggag gcaaaatgag
    28801 caacggcatt gtgatcattg gttcgggctt cgccgcccgc caactggtga aaaatattcg
    28861 caaacaggac gccagtattc cattaaccct gattgccgcc gacagtatgg atgagtacaa
    28921 caaacctgac ctcagccatg ttatcagtca ggggcaacgt gccgacgacc ttacccgcca
    28981 gacggcgggt gaatttgccg agcagtttaa tctgctcctg tttccgcaaa cctgggtgac
    29041 ggatatcgat gccgaagccc gtgtggtgaa aagccagaat aatcagtggc aatacgacaa
    29101 gctggtgctg gcaaccggtg ccagcgcctt tgtcccgcca gtgcctgggc gtgagttaat
    29161 gctgacgtta aatagtcagc aagagtatcg cgcctgtgaa acgcaactgc gggatgcccg
    29221 acgcgtgttg attgttggcg gtggtttgat tggtagcgaa ctggcgatgg atttttgtcg
    29281 tgcaggcaaa atggttacgc taatcgacaa cgctgccagt attctggcgt cgttaatgcc
    29341 accggaagta agcagtcgct tgcagcatcg gttgacggag atgggcgttc atctgctgtt
    29401 gaaatctcag ttgcaggggc tggaaaaaac ggattctggc attctggcaa cgctggacca
    29461 ccagcgcagc atcgaagtgg atgcggtaat tgccgccacc ggactgcgcc cggaaaccgc
    29521 cctggcacga cgcgccgggc tgacgattaa tcgcggcgtt tgcgtcgata gttatctgca
    29581 aaccagtaat gccgatattt acgcgctggg cgattgcgcg gaaattaacg gtcaggtatt
    29641 gccgttcctg cagccgattc aacttagcgc gatggtgctg gcaaaaaatc ttctcggcaa
    29701 taacacgccg ctgaaactac cggcgatgct ggtgaaaatc aaaacgccgg aattaccgct
    29761 gcatctggca ggcgaaaccc agcgtcagga tttacgctgg cagattaata ccgaacgcca
    29821 gggaatggtg gcgcgcggtg ttgacgatgc agaccaactt cgcgcctttg tggtcagtga
    29881 ggatcggatg aaagaggcat ttggattgtt gaaaacgttg ccggtgtagg tgggctactg
    29941 tgcctaaaat gtcggatgcg acgctggcgc gtcttatccg acctacgggg acgcatgtgt
    30001 aggccggata aggcgtttac gccgcatccg gcaatggtgt ccaaatgcaa cacgttttat
    30061 ccgttctgga cttcacccgc taaccaacgc gccgcagcaa taaccccctg ccccagagac
    30121 aaaccgccat cacccgccgg taaactctgt ggaaacagca atgtgaaatc agcgagataa
    30181 tgcgccagac gtgcacgcag caaacggtta tgaataaccc cgccgctaaa taccagcgta
    30241 gtgataccac gcatcgtggc ctgctcacgc atcaacgcgg caaaaccctg cgccagcgca
    30301 tcatgaaacg cccacgcgcg ttgattaacc ggtgcctgcc agttcagcca ctgctgccag
    30361 aaagtggcga gatccagttg attgtccacc cgcggcatcg tcaccggatg cgtcactccg
    30421 tcgcatgagg ctgctagcgc ctccagagca caagccgctt caccttcata acttaacgtg
    30481 gctggcgcac agcccagtgc cgccgccaca gcatcgaaca aacgcccaca cgatgacgcc
    30541 agcggcgcgt taattccacg ctcaatggcc cgcgccagta cgctccagtt ttgctgttgc
    30601 acacttgccg tttcagggta attctgccac tccggcacaa agcgcaggca ctgcgccagc
    30661 aggttacgcc acggctgctt cgccgccaaa tcgccacccg gaagcgccac tgcaggcaag
    30721 ccgcccaggt gctcacattc gcgatagttc acccgcaggc actcgccgcc ccacaaagcg
    30781 ccgttctccc ccataccgat accgtcgagc gtcaaagcaa tgacatcacc gccatccagc
    30841 ggccactgat gctctgccag acacgccgct gcgtgggcat gatgatgcag caccgtttgc
    30901 gtcggcagat tcatttcgct cgcccactgg caggagacat agcccggatg tgcgtcatgc
    30961 acaacgtatt gcggagtgaa attgtagatg ttttgcatca ggcgtaacgc ttcgcgccac
    31021 tgcgtctgga tgccatcgtc acttaaatcg cccagatgct ggctcaacac cacttgttca
    31081 ccgcgcacca ggcagaaggt atttttcaga tccgcgccga gacacagtac aggcggaaca
    31141 tttttaaagc ccggaggcaa agccagcgca tccggcacat acccccgcga acggcgcagc
    31201 atttcgccgc tttcgcgcac caccgaatca tccatccgct gcacaatgtc gcggttgtgt
    31261 attaagaatc cgtcggcaat gccctgcaaa tcctccagcg cctgttcgtt gctgatagcc
    31321 ggtggtttac cgctcaggtt gccagaggtc atcaccagcg ggcattgtag ttcctgtaac
    31381 agcaaatgct gaagcggatt cgcaggcaac attaccccga cttcgttaag gccaggggcg
    31441 atatcatcac aaagctcagg aacgtatttt ttatccacca gcacaatcgg cgcggcgggc
    31501 gtggtaagca actggcgcgc agcgtctggt aaaccgtcag ccactggcaa catgactgcc
    31561 agcggtttcg ccgggcgatg tttgcgcgcc cgaagtgtcg ccaccgcgtt actgttacgt
    31621 gcatcgcagg caagatgaaa tccgccaatc cctttgatgg cgacaatgtt gcccattttt
    31681 aactgtgcga tagccgcctg taatgccgcc tcttgttctg catgttcacc atgacttacc
    31741 cattcaagat acgggccaca ctccgggcag gccaccggct gggcgtggaa gcgacgatcg
    31801 agcgggtcgc ggtactcttt atcacaggct ggacacagcg gaaacgccgc catcacggta
    31861 aacgggcgat cgtaaggcat ggcgcgaata atggtgaagc gcgggccgca gtgggtacag
    31921 ttgataaatg gataacgata acgccgttcg cctggggtat tcatttcggc aaggcaagca
    31981 gggcaagtgg cggcatcggg aacaatttgc gtattcatgg cgccgcccgc gctctggcgg
    32041 atggtgaact cagtgggcag ttgtgaccag ataaacggtt cacgctcaac gctatcaata
    32101 cgcgccagcg gcgggcagtg ctgatgcaat tgaacaagaa acgtttccgg gtcttccagc
    32161 agccggactt ctacgccatc gccgtcatta cagacatcgc cgtgaagatt taattgctgt
    32221 gccagctgcc agacaaacgg acgaaaaccg acgccctgca ctttgcctcg aatacgcagt
    32281 tggacaccgc aagatgtgtt ttttgccatt gagttattcc cgccatcatg aattgcgtaa
    32341 cccgtccagc cggacacgac agcgtcgcat ccggcagtca caggtcggcg ataccgcccg
    32401 ctccgtattc tacgaatatt tccgggaatt cctttgatgc cagaacagtt ctgtaagatt
    32461 tttagaacat cagcgccgta cggcggcgtt tttctgcgct cagttgttca agtttattgc
    32521 gatcgacaca aattagcgca tgggtcgggc aagccgccat acacgccggg ccgtcttcac
    32581 gatggttgca caggtcgcat ttattggctt cggctttgtc agcccgtaca ttcagccctg
    32641 cgccgctgtt gcgaatcacc ggacgtacca ccacttccat cgcgccatac gggcaagcca
    32701 caacgcaggt tttgcaacca atgcaacgtt cctgcatcac atgaacaaac cctttatcac
    32761 ggctgatagc accattcggg cagacgttag cgcacggtgc atcttcacac tgacggcaaa
    32821 ctgtcgccgt ggaaatgttc acacctttaa tgacatggat acgcggtaaa aatgtttccg
    32881 gggtcagcga tgcacagtcc tgattttcct gatgagaaac cacgcacgcc acttcacagg
    32941 tacggcaacc aatacattta ctcgcgtcag caatgatgaa acggttcatc aaattctcca
    33001 gcaatgacag ttaatgcgcc gatacattca caaatcatgc cagtttttaa tttgctgtta
    33061 tttaaggaaa ttaatttctg taatgcagga aaaaagatgt catcgacact agtgacgatg
    33121 acatgtgatg acaatgttta tcgcgaaaga gcaatgagtg agtcgcggcg gatcagtttt
    33181 ccgctgaagg ttttcggcgg tgagaaatcc ccgccatcga gcataaaaat cagccgtcca
    33241 ataatttcct gaatcatctc agtcaccgga atttttacgc tggagagcgc cggaacgata
    33301 tagggggcaa ttgcgatatc atcgaatccg ataactgaca cctgctctgg caccgctacg
    33361 ccgcgcttgt gtaacgcttt catcgcacct atcgccatat cgtcgttact ggcaactaac
    33421 gcgctaaatt tagccccacg ttcgagcaac gtttctaccc cttcggcccc gctggcgggc
    33481 gtccatttac cgttagcgat aagtttttca ttgagcgcaa taccatgctg cgccagcgcg
    33541 tctttatatc cggcaagacg ttcaacgctg gtgggggaat ccatcgagcc ggtaagaaaa
    33601 gcaatctcct gatgcccggc gtttatcaac tctgccacgg cgttaaagct ggtctgttta
    33661 tgatcgcacc agacgctatg gctgctattt ttgcgcaggc ggcgattaag caccattatc
    33721 ggctgactgt gcgcgtcaat gatgtcatcg atctcatcca cacttaaaaa acgcgggtaa
    33781 atcataatgg cgtcgcagcg caaatccagc agatactgaa tcgcctggcg ctcttcttcg
    33841 gcgctgtgtt taccatctgc caatagcaac tgccgccctt tctcttccgc cattcgcgcg
    33901 gcatgaaaca gtaattcact aaaataaatg ccgtggtaaa gcgtgttagt cactaccagc
    33961 cccagcgtct gggtactttt cgccgacaga ttgcgcgcca gcaagtttgg acggtaaccg
    34021 ctctcttcta ccgcctgaaa cacgcgatct ttagtctcct ggctgacgta gccattacct
    34081 gaaagcacgc gggaaacggt cgcttttgaa accccggcgc gcttcgccac ttccagcatc
    34141 gtcgtcatca ttttcatccc tttacacgca atcaacgcag tgtactgcac cgtttaccga
    34201 ttgtccttgc gcaatcggcg ggaaaaatat tcaggtgacc ggttt
//
LOCUS       DBFIFM010000028        34213 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.028, whole genome shotgun sequence.
ACCESSION   DBFIFM010000028 DBFIFM010000000
VERSION     DBFIFM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.028"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(1..698)
                     /locus_tag="N7C74_001210"
                     /pseudo
     CDS             complement(1..698)
                     /locus_tag="N7C74_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(843..1451)
                     /locus_tag="N7C74_001211"
     CDS             complement(843..1451)
                     /locus_tag="N7C74_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HGU2431112.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1511..1828)
                     /gene="metJ"
                     /locus_tag="N7C74_001212"
     CDS             complement(1511..1828)
                     /gene="metJ"
                     /locus_tag="N7C74_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HGU2431113.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2105..3265
                     /gene="metB"
                     /locus_tag="N7C74_001213"
     CDS             2105..3265
                     /gene="metB"
                     /locus_tag="N7C74_001213"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HGU2431114.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAENPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAKAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3268..5700
                     /gene="metL"
                     /locus_tag="N7C74_001214"
     CDS             3268..5700
                     /gene="metL"
                     /locus_tag="N7C74_001214"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HGU2431115.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPTSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(5839..5973)
                     /locus_tag="N7C74_001215"
     CDS             complement(5839..5973)
                     /locus_tag="N7C74_001215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2431116.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6049..6939
                     /gene="metF"
                     /locus_tag="N7C74_001216"
     CDS             6049..6939
                     /gene="metF"
                     /locus_tag="N7C74_001216"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HGU2431117.1"
                     /translation="MNFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7268..9448
                     /gene="katG"
                     /locus_tag="N7C74_001217"
     CDS             7268..9448
                     /gene="katG"
                     /locus_tag="N7C74_001217"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HGU2431118.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9542..10447
                     /gene="yijE"
                     /locus_tag="N7C74_001218"
     CDS             9542..10447
                     /gene="yijE"
                     /locus_tag="N7C74_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HGU2431119.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAAVFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(10474..11091)
                     /locus_tag="N7C74_001219"
     CDS             complement(10474..11091)
                     /locus_tag="N7C74_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HGU2431120.1"
                     /translation="MKASLALVSLLTAFTSYSLKSPAIPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11365..12468)
                     /gene="gldA"
                     /locus_tag="N7C74_001220"
     CDS             complement(11365..12468)
                     /gene="gldA"
                     /locus_tag="N7C74_001220"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HGU2431121.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENSPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12479..13141)
                     /gene="fsa"
                     /locus_tag="N7C74_001221"
     CDS             complement(12479..13141)
                     /gene="fsa"
                     /locus_tag="N7C74_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGU2431122.1"
                     /translation="MELYLDTANVAEVERLAHIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13153..15654)
                     /gene="ptsP"
                     /locus_tag="N7C74_001222"
     CDS             complement(13153..15654)
                     /gene="ptsP"
                     /locus_tag="N7C74_001222"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGU2431123.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFSRSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            15963..17042
                     /locus_tag="N7C74_001223"
     CDS             15963..17042
                     /locus_tag="N7C74_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HGU2431124.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17057..17377
                     /locus_tag="N7C74_001224"
     CDS             17057..17377
                     /locus_tag="N7C74_001224"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HGU2431125.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            17428..19725
                     /locus_tag="N7C74_001225"
     CDS             17428..19725
                     /locus_tag="N7C74_001225"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGU2431126.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELGQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVGNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            19691..20569
                     /locus_tag="N7C74_001226"
     CDS             19691..20569
                     /locus_tag="N7C74_001226"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HGU2431127.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20571..20912
                     /locus_tag="N7C74_001227"
     CDS             20571..20912
                     /locus_tag="N7C74_001227"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HGU2431128.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQQVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(20899..21750)
                     /locus_tag="N7C74_001228"
     CDS             complement(20899..21750)
                     /locus_tag="N7C74_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2431129.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(21965..23698)
                     /locus_tag="N7C74_001229"
     CDS             complement(21965..23698)
                     /locus_tag="N7C74_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HGU2431130.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNMFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELNALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKENTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(23881..26532)
                     /gene="ppc"
                     /locus_tag="N7C74_001230"
     CDS             complement(23881..26532)
                     /gene="ppc"
                     /locus_tag="N7C74_001230"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HGU2431131.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKTLWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(26884..28035)
                     /gene="argE"
                     /locus_tag="N7C74_001231"
     CDS             complement(26884..28035)
                     /gene="argE"
                     /locus_tag="N7C74_001231"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HGU2431132.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASTGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICAWCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            28189..29193
                     /gene="argC"
                     /locus_tag="N7C74_001232"
     CDS             28189..29193
                     /gene="argC"
                     /locus_tag="N7C74_001232"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HGU2431133.1"
                     /translation="MLNTLIVGASGYAGAELVTCVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLDAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29201..29977
                     /gene="argB"
                     /locus_tag="N7C74_001233"
     CDS             29201..29977
                     /gene="argB"
                     /locus_tag="N7C74_001233"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HGU2431134.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30038..31411
                     /gene="argH"
                     /locus_tag="N7C74_001234"
     CDS             30038..31411
                     /gene="argH"
                     /locus_tag="N7C74_001234"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HGU2431135.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEELPLTELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FARARLG"
     gene            31678..32595
                     /gene="oxyR"
                     /locus_tag="N7C74_001235"
     CDS             31678..32595
                     /gene="oxyR"
                     /locus_tag="N7C74_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HGU2431136.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(32578..33978)
                     /gene="sthA"
                     /locus_tag="N7C74_001236"
     CDS             complement(32578..33978)
                     /gene="sthA"
                     /locus_tag="N7C74_001236"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HGU2431137.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
BASE COUNT         7725 a         8644 c         9544 g         8300 t
ORIGIN      
        1 tccaattagt agaacatata ttttcgataa aagcaccgat gactttttca tgaagttcta
       61 cagaactcga gaaacagatg gttctgcggg ccagacgttt gattcgggtt ctcagggtca
      121 gattgttacg ctcaatgtgc tgagtaaaaa tcttaccagt cagatgcttc tcctttggta
      181 actctctagc gtaactaccc cattcgtcag tggtcaacat gccgatggta aaagggtaag
      241 cagcgccagc aattcacgac aggtttcatc agtccggggg ccaaaagtgt aggccagaac
      301 accaccagtt ttcgtgttgt acacgtacca gagccagtgc tgtcgcgctt tactgccgac
      361 aaagctccat tgctgactaa gtttgcagat gagtgccaca tcagcatgag cgaccgggga
      421 tgacgttatt cttcgtggcg agagtttttt aaagttcgga tgactgtatt aatgccgatt
      481 ttcagcgtac gggcagtata gcgaaccccg gcaccgttga aagccatttc agcgatttgg
      541 tctttaactc ccggtttacg gggctcgtaa cagtaactga gctgaaatac ccggtgacag
      601 tcacggccgc ggaatcgatc atgtcttttg gggttctgcc cgcgacggta aacctgaacg
      661 gactggcaac ggggacaatg aaggttaact gtgaccatga aacatcctta aaagcgagta
      721 ttatatcctt aattcaacta attggaggca tcaccgttta ccggagcgac agcttgcgga
      781 aaggtcgtgc aactacaaag caacaaacag actacggcgg gaattatttt tttcatcatc
      841 cattaccacg aaaacagcgg ccattctttg gcaaccgtgg tgagttgtgg cgcatcgaaa
      901 acggcttgtg gtgagaccac cgtttcctca agcggaatat ttttgccgta gcgttctttg
      961 agcttcgcct ggaccagcgg gttgctgaga tcaaactctt tcaacttctg ctgttcgccc
     1021 acacgcattc ccagcgcatt ctgataaacc ttccacacca cttccgaaca gtactggcga
     1081 tcgtcgctcc atgagaagct gaaatcgtac ggtttgccga gataacgttt cgccgtttgc
     1141 gccagttttt gctgttgttc aacactcagt ccgccttcaa cgcggcgaac aacgtatttg
     1201 cccttttcac catgggcaat ccactgcttg agcggggtgt atttcaccgg gccgaccgct
     1261 tcaaaaacgt agggcttttt gttacgcatc accagcatac cggtgtggct gtaatcggaa
     1321 tgggtcgcca gttggatcgc tttactttgc gatgagcgag agatttgaaa gatgatgtcg
     1381 ccagtttgcg gctgccaggc aaaggcaggc acagaaacca gcaggctgag gatcagtagc
     1441 ctgattttca tcgttaaacg tccttgtatg aaaaaagcgg ctggttaact ccgcgccgct
     1501 cttttccgct ttagtattcc cacgtctccg ggttaatccc catctcacgc atgatctctt
     1561 ttgccgcttc cgggatttcg tcgctgcgct ctttacgcag atcggcatca tccggcaaag
     1621 gttgcccggt aaaggcatgc agaaacgctt cgcacagcag ctcgctgttg gtagcgtgac
     1681 gcaggttgtt cacctgacga cgcgtgcgtt catcggtgag gatttttaac accttaagag
     1741 gaatggaaac cgtaatcttt ttgacttgtt cactcttctt gccgtgctca gcgtatgggc
     1801 tgatatattc gccgctccat tcagccatga gatacttaat cctcttcgtc aataaattga
     1861 gaccagacca cacaatttat gtgggtactg accgtaaacc cgcatagttt accgtacagg
     1921 cgttaccgtg acatcgtgta atgcacctgt cggcgtgata atgcatataa ttttaacggc
     1981 tatttgggat ttgctcaatc tatacgcaaa gaagtttaga tgtccagatg tattgacgtc
     2041 cattaacaca gtgtttactc tggtgcctga catttcaccg accaagccca gggaacttca
     2101 tcacatgacg cgtaaacagg ccaccatcgc agtgcgtagc gggttaaatg acgacgaaca
     2161 gtatggttgc gttgtccctc ccatccatct ttccagcacc tataacttta ccggatttaa
     2221 tgaaccgcgc gcgcatgatt actcgcgtcg cggcaaccca acgcgcgatg tggttcagcg
     2281 tgcgctggca gaactggaag gcggtgctgg tgcagtactt actaataccg gcatgtccgc
     2341 gattcatctg gtaacgaccg tctttttgaa acctggcgat ctgctggttg cgccgcacga
     2401 ctgctacggc ggtagctatc gcctgttcga cagcctggcg aaacgcggtt gctatcgcgt
     2461 gttgtttgtt gatcaaggcg atgaacaggc attacgggca gcgctggcag aaaaccccaa
     2521 actggtactg gtagaaagcc caagtaatcc attgttacgc gtcgtggata ttgcgaaaat
     2581 ctgccatctg gcaagggaag tcggggcggt gagcgtggtg gataacacct tcttaagccc
     2641 ggcattacaa aatccgctgg cattaggtgc cgatctggtg ttgcattcat gtacgaaata
     2701 tctgaacggt cactctgacg tggtggctgg cgtggtgatt gctaaagacc cggacgttgt
     2761 cactgaactt gcctggtggg caaacaatat tggcgtgacg ggcggcgcgt ttgacagcta
     2821 tctgctgcta cgcgggttgc gaacgctggt gccgcgtatg gagctggcgc agcgcaacgc
     2881 gaaggcgatt gtgaaatatc tgcaaaccca gccgttggtg aaaaaactgt atcacccgtc
     2941 gctgccggaa aatcaggggc atgaaattgc cgcgcgtcag caaaaaggct ttggcgcaat
     3001 gttgagtttt gaactggatg gcgatgagca gacgctgcgc cgtttcctgg gcgggctgtc
     3061 gttatttacg ctggcggaat cattaggggg agtggaaagt ttaatctctc acgccgcaac
     3121 catgacacat gcaggcatgg caccagaagc gcgtgctgcc gccgggatct ccgagacgct
     3181 gctgcgtatc tccaccggta ttgaagatgg cgaagattta attgccgacc tggaaaatgg
     3241 cttccgggct gcaaacaagg ggtaaaaatg agtgtgattg cgcaggcagg ggcgaaaggt
     3301 cgtcagctgc ataaatttgg tggcagtagt ctggctgatg tgaagtgtta tttgcgtgtc
     3361 gcgggcatta tggcggagta ctctcagcct gacgatatga tggtggtttc cgccgctggt
     3421 agcaccacta accagttgat taactggttg aaactaagcc agaccgatcg tctctctgcg
     3481 catcaggttc aacaaacgct gcgtcgctat cagtgcgatc tgattagcgg tctgctaccc
     3541 gccgaagaag ccgatagcct cattagcgct tttgtcagcg atcttgagcg cctggcggcg
     3601 ctgctcgaca gcggtattaa cgacgcagtg tatgcggaag tggtgggcca cggcgaagtg
     3661 tggtcggcgc gcctgatgtc tgcggtgctt aatcaacaag ggctgccagc ggcctggctt
     3721 gatgcccgcg agtttttacg cgctgaacgc gctgcgcaac cgcaggttga tgaagggctt
     3781 tcttacccgt tgctgcaaca gctactggta caacatccgg gtaaacgtct ggtggtgacc
     3841 gggtttatca gccgtaacaa cgccggtgaa acggtgctgc tggggcgtaa cggttccgac
     3901 tattccgcga cacaaatcgg tgcgctggcg ggtgtttctc gtgtaaccat ctggagcgac
     3961 gtcgccgggg tatacagtgc cgatccgcgt aaagtgaaag atgcctgtct gctgccattg
     4021 ctgcgtctgg atgaagccag cgaactggcg cgtctggcgg ctcccgttct tcacgcccgt
     4081 actttacagc cagtatccgg tagcgaaatc gacctgcagc tgcgctgtag ctacacgccg
     4141 gatcaaggct ccacgcgcat tgaacgcgtg ctggcctccg gtactggtgc gcgtattgtc
     4201 accagccacg atgatgtctg cttgattgag tttcaggtgc ccaccagcca ggatttcaaa
     4261 ctggcgcata aagagatcga ccagatcctg aaacgtgctc aggtgcgccc actggcggtt
     4321 ggcgtgcata acgatcgcca gttgctgcaa ttttgctaca cctcagaagt ggccgacagt
     4381 gcgctgaaaa tcctcgacga agcgggatta cctggcgaac tgcgcctgcg tcaggggctg
     4441 gcgctggtgg cgatggtcgg ggcgggtgtc acccgtaacc cgctgcattg ccaccgcttc
     4501 tggcagcaac tgaaaggcca gccggtcgag tttacctggc agtccgatga cggcatcagc
     4561 ctggtggcag tactgcgcac cggcccgacc gaaagcctga ttcagggcct gcatcagtcc
     4621 gtcttccgcg cagaaaaacg catcggcctg gtattgttcg gcaaaggcaa tatcggttcc
     4681 cgctggctgg aactgttcgc ccgcgagcag agcacgcttt cggcgcgtac cggctttgaa
     4741 tttgtgctgg caggcgtggt ggacagccgc cgcagtctgt tgagttatga cgggctggac
     4801 gccagccgcg cgttagcctt cttcaacgat gaagcggttg agcaggatga agagtcgttg
     4861 ttcctgtgga tgcgcgccca tccgtatgat gatttagtgg tgctggacgt taccgccagc
     4921 cagcagctgg ctgaccagta tcttgatttc gccagccacg gtttccacgt catcagtgcc
     4981 aacaaactgg cgggagcaag cgacagcaat aaatatcgcc agatccacga cgccttcgaa
     5041 aaaaccgggc gtcactggct atacaacgcc accgtcggtg cgggcttgcc gatcaaccat
     5101 accgtgcgcg atctgatcga cagcggcgat actattttgt cgatcagtgg gatcttctcc
     5161 ggcacgctct cctggctgtt cctgcaattc gacggtagcg tgccgtttac cgagctggtg
     5221 gatcaggcgt ggcagcaggg cttaaccgag cctgatccgc gtgacgacct ctccggcaaa
     5281 gacgtaatgc gcaagctggt gattctggcg cgtgaagcgg gttacaacat tgaaccggac
     5341 caggtacgtg tggaatcgct ggtgcctgct cattgcgaag gcggcagcat cgaccatttc
     5401 tttgaaaatg gcgatgaact taacgagcag atggtgcaac ggctggaagc ggcccgcgaa
     5461 atggggctgg tgctgcgcta cgtggcgcgt ttcgatgcca acggcaaagc gcgtgtgggc
     5521 gtggaggcgg tgcgtgaaga ccatccgctg gcatcactgc taccgtgcga taacgtcttt
     5581 gccatcgaaa gccgctggta tcgcgacaac ccgctggtga tccgtgggcc tggcgcgggg
     5641 cgcgacgtca ccgccggggc gattcagtcg gatatcaacc ggctggcaca gttgttgtaa
     5701 tttcagaaat ttaataatgc ccggtactca tgttttcggg tttatggttt ctaatgaaat
     5761 atattgaatt atcataggat taggccggat gaagcgttta cgacgaatcc ggcaagaagc
     5821 aataagtaca tggttagttt atatttgcag tccggtttgc tttgcatacc ggattttctt
     5881 tttcttacca tcctgaagtt ttttcatctt ccctgatttt tcctcaccat cattggtcat
     5941 ttttcggttg acgcccttcg gcttttcctt catctttaca tctggacgtc taaacggatg
     6001 gatgtgcaca acacaacata taactacaag cgattgatga ggtaaggtat gaactttttt
     6061 cacgccagcc agcgggatgc cctgaatcag agcctggcag aagtccaggg gcagattaac
     6121 gtttcgttcg agtttttccc gccgcgtacc agtgaaatgg agcagaccct gtggaactcc
     6181 atcgatcgcc ttagcagcct gaaaccgaag tttgtatcgg tgacctatgg cgcgaactcc
     6241 ggcgagcgcg accgtacgca cagcattatt aaaggcatta aagatcgcac tggtctggaa
     6301 gcggcaccgc atcttacttg cattgatgcg acgcccgacg agttgcgcac cattgcacgc
     6361 gactactgga ataacggtat tcgtcatatc gtggcgctgc gtggcgatct gccgccggga
     6421 agtggtaagc cagaaatgta tgcttctgac ctggtgacgc tattaaaaga agtggcagat
     6481 ttcgatatct ccgtggcggc gtatccggaa gttcacccgg aagcaaaaag cgcccaggcg
     6541 gatttgctta acctgaaacg caaagtcgat gcaggagcca accgcgcgat tactcagttc
     6601 ttctttgatg tcgaaagcta cctgcgtttt cgtgaccgct gtgtgtcggc gggcattgat
     6661 gtggaaatca ttccgggcat attgccggta tctaacttta aacaggcgaa gaaatttgcc
     6721 gacatgacca acgtgcgtat tccggcgtgg atggcgcaaa tgtttgacgg gctggatgac
     6781 gatgccgaaa cccgcaagct ggtgggcgcg aatattgcca tggatatggt gaagatttta
     6841 agccgtgaag gagtgaaaga tttccacttc tatacgctta accgcgctga aatgagttac
     6901 gcgatttgcc atacgctggg ggttcgacct ggtttataaa tagtgtggct tttatgaaaa
     6961 tcacacagtg atcacaaatt ttaaacagag cacaaaatgc tgcctcgaaa tgagggcggg
     7021 aaaataaggt tatcagcctt gttttctccc tcattacttg aaggatatga agctaaaacc
     7081 cttttttata aagcatttgt ccgaattcgg acataatcaa aaaagcttaa ttacgatcaa
     7141 tttgatctac atctctttaa ccaacaatat gtaagatctc aactatcgca tccgtggatt
     7201 aattcaatta taacttctct ctaacgctgt gtatcgtaac ggtaacactg tagaggggag
     7261 cacattgatg agcacgtcag acgatatcca taacacgaca gccactggca aatgcccgtt
     7321 ccatcagggc ggtcacgacc agagcgcggg ggcgggcaca accactcgcg actggtggcc
     7381 aaatcaactc cgtgttgacc tgttaaacca acattctaat cgttctaacc cgctgggtga
     7441 ggactttgac taccgcaaag aattcagcaa attagattac tacggcctga aaaaagatct
     7501 gaaagccctg ctgacagaat ctcagccgtg gtggccagcc gactggggca gctacgccgg
     7561 tctgtttatt cgtatggcct ggcacggcgc ggggacttac cgatcaatcg atggacgcgg
     7621 tggcgcgggt cgtggtcagc aacgttttgc accgctgaac tcctggccgg ataacgtaag
     7681 cctcgataaa gcgcgtcgct tgttgtggcc aatcaaacag aaatatggtc agaaaatctc
     7741 ctgggccgac ctgtttatcc tcgcgggtaa cgtggcgctg gaaaactccg gcttccgtac
     7801 cttcggtttt ggtgccggtc gtgaagacgt ctgggaaccg gatctggatg ttaactgggg
     7861 tgatgaaaaa gcctggctga ctcaccgtca tccggaagcg ctggcgaaag caccgctggg
     7921 tgcaaccgag atgggtctga tttacgttaa cccggaaggc ccggatcaca gcggcgaacc
     7981 gctttctgcg gcagcagcta tccgcgcgac cttcggcaac atgggtatga acgacgaaga
     8041 aaccgtggcg ctgattgcgg gtggtcatac gctgggtaaa acccacggtg ctggtccgac
     8101 atcaaatgta ggtcctgatc cagaagctgc gccgattgaa gaacaaggtt taggttgggc
     8161 gagcacttac ggcagcggcg ttggcgcaga tgccattacc tctggtctgg aagtggtctg
     8221 gacccagacg ccgacccagt ggagcaacta tttcttcgag aacctgttca agtatgagtg
     8281 ggtacagacc cgcagtccgg ctggcgcaat ccagttcgaa gcggtagacg caccggaaat
     8341 tatcccggat ccgttcgatc cgtcgaagaa acgtaaaccg acgatgctgg tgaccgacct
     8401 gacgctgcgt tttgatcctg agttcgagaa gatctctcgt cgtttcctca acgatccgca
     8461 ggcgttcaac gaagcctttg cccgtgcctg gttcaaactg acgcacaggg atatggggcc
     8521 gaaatctcgt tacatcgggc cggaagtacc gaaagaagat ctgatctggc aagatccgct
     8581 gccgcagccg atctacaacc cgaccgagca ggacattatc gatctgaaat tcgcgattgc
     8641 ggattctggt ctgtctgtta gtgagctggt atcggtggcc tgggcttctg cttctacctt
     8701 ccgtggtggc gacaaacgcg gtggtgccaa cggtgcgcgt ctggcattaa tgccgcagcg
     8761 cgactgggat gtgaacgccg cagccgttcg tgctctgcct gttctggaga aaattcagaa
     8821 agagtctggt aaagcctcgc tggcggatat catcgtgctg gcaggtgtgg ttggcgttga
     8881 gaaagccgca agcgccgctg gtttgagcat tcatgtaccg tttgcgccgg gtcgcgttga
     8941 tgcgcgtcag gatcagactg acattgagat gttcgaactg ctggagccaa ttgctgacgg
     9001 tttccgtaac tatcgcgctc gtctggatgt ttccaccacc gagtcactgc tgattgataa
     9061 agcccagcaa ctgaccctga ccgcgccgga aatgaccgcg ctggtgggcg ggatgcgtgt
     9121 actgggtgcc aacttcgatg gcagcaaaaa cggcgtcttc actgaccgcg ttggcgtatt
     9181 gagcaatgac ttcttcgtga acttgctgga tatgcgttac gagtggaaag cgaccgacga
     9241 atcgaaagag ctgttcgaag gccgtgaccg cgaaaccggc gaagtgaaat acactgccag
     9301 ccgtgcggat ctggtatttg gttctaactc tgtcctgcgt gcggtggctg aagtttacgc
     9361 cagcagcgat gctcacgaga agtttgttaa agacttcgtg gcggcatggg tgaaagtgat
     9421 gaacctcgac cgtttcgacc tgctgtaatc tgaccttctt cagcgactgc ctttcaggca
     9481 gtcgctgaag tttctttacc ggcgtatagt gtccacagga aaactacaca ctggatctct
     9541 catgtctgcc gcaggaaaga gcaacccact ggcaatcagt ggcctggttg tgctcacact
     9601 tatctggagt tatagctgga ttttcatgaa gcaagtcacc agctacatcg gtgccttcga
     9661 ctttaccgcc ttacgctgca ttttcggcgc tctcgtttta ttcatcgtcc ttttattacg
     9721 tggtcgcgga atgcgcccga caccgtttaa atacacctta gccattgccc tgttacaaac
     9781 ctgcgggatg gttggcctgg cgcagtgggc gctggtcagc ggcggagcgg ggaaggtggc
     9841 gatcctgagc tataccatgc cgttctgggt ggtgattttc gccgcggtgt ttctcggtga
     9901 acgcctgcga cgtgggcaat atttcgcgat tctgattgcc gctttcggct tatttttggt
     9961 gttgcagccg tggcaactcg atttctcttc gatgaaaagt gccatgctgg caatcctctc
    10021 cggtgttagt tggggggcga gcgcgattgt tgctaaacgt ctgtatgccc gtcatccgcg
    10081 cgtggattta ttgtcgttaa catcctggca gatgctctat gcggcgctgg tgatgagtgt
    10141 ggtcgcttta ctggtgccgc aacgtgaaat tgactggcag cccactgtgt tctgggctct
    10201 ggcctacagt gcgattctgg cgacggcgtt ggcgtggagc ttatggctgt ttgtgctgaa
    10261 aaacttgcct gccagtattg ccagcttaag cacactggcc gttcccgttt gcggcgtact
    10321 cttttcctgg tggctgctcg gtgagaatcc gggggccgtt gaaggtagcg gtattgtgct
    10381 gattgtgctg gcactggcgc tggtgagccg taagaaaaaa gaagccgtta gtgtaaaaag
    10441 gatctgaatt ttttcttcat gtggggcgat cgcttattta acaaaataac gataatgccc
    10501 caccatgcgc cagctaaaca gcacatcttc ttcctgcgca cctgcgccaa tgttatgtat
    10561 caccagcggc gtaccgtcgc gggcgaagcc atctgaaacc accccaatat gtgccagccc
    10621 gttatctaat cgccaggaga caatatcgcc cgcttgatag tcactggggt tcttgctgat
    10681 ggggcgtgtt ttatcgtggc gggtaaacca ggtttccaga ttaggcaccc gacggtgatc
    10741 gatgttgctg tccgggcgct ttaactgcca tttttgcggg tactcggcaa aattcttcgc
    10801 catatcttcg tgcaccagtt tctgcaaatc gaccttctgg ctgcgcaatg cacggatcac
    10861 cacatcggaa cataccccgc gttcttgcgg gacatcaccg ccaggatagg taagctgcac
    10921 atacgccgga tcgtaaaata gcgtgctgcc gatttgctgt ctggcaccgt ctgcgatggc
    10981 aaggttggta ttggcctgga tttgtaccac ggttggtgga atggcgggag attttaagga
    11041 gtagctggta aatgccgtta gcaggctgac cagcgccagt gaagctttca tctgtttcgt
    11101 ccgtgaaaat aagagtcaca gattcgacct tccgggctaa cgattgttct gtagagaaaa
    11161 tgtccagaag cggataaaaa attcctgaac aacgtgggag tttcagatta ctggcacaga
    11221 gtgccggatg cggcgtgaac gtcttatctg gcctaaaaag cacgcaaatt caatatattg
    11281 cagagattgc gtaggcctga taagcgtagc gcatcaggca attttgcgtt caaactcccg
    11341 gacaagccgg gagtttggag taggttattc ccactcttgc aggaaacgct gaccgtactg
    11401 gtcggctacc agcagagcgg cgtaaacctg atctggcgtc gcgccgccag gcatgttgtg
    11461 gatggtttca ccttctgcac atgccgcttc tgccacaatt cgcattttcg ccgggacatc
    11521 ttctttaata tccagttgag cgagagttat tggcaaacct accgcatggc taagcgcagc
    11581 tacggtttcg atttcctcaa ccggcgagtt ttccagtacc agctgcgtca gtgtaccaaa
    11641 tgccactttt tcaccgtgat aatagtgatg ggcatcagga atggcagtca ggccattatg
    11701 caccgcgtgc gctgccgcca gaccaccgct ttcaaagcca acaccgctta aataagtgtt
    11761 cgcttcaatc acgcgttcca gtgccggagt cactacatgc tgttcggcgg cgagcatcgc
    11821 tttttcgcct tcttccagca aggtgttgta gcacagttca gccagcgcca gcgccgcctg
    11881 agtgcacttg ccgcccgcca tggtggtcgc accgctacga gagcaggcac gcgcttcaaa
    11941 ccaggtcgcc agcgcatcgc cgatacccgc cgctaacaga cgcgcaggtg cgcccgcgac
    12001 gattttagta tcgacaatca ccatattcgg gttatttggc agcagcagat agcggtcaaa
    12061 ttcaccttcg tcagtgtaga taacggaaag agcgctacaa ggtgcgtcgg ttgaagcaat
    12121 ggtcggggca atggcgaccg gaacacccat gaaatgtgcc agtgctttgg ctgtatcgag
    12181 ggtttttccg ccaccgatac cgagaattgc gccacactgc gcagtctccg cgatgccacg
    12241 cagacggtcg atctcatttt gcgaacattc accgccaaac ggcgcaatct ctactaccag
    12301 tccagcatct ttaaagcttt tctcgacagt ggattgagca aaacctaaaa caaatttgtc
    12361 acccaccact aaccagcgtt ctgccagcgg cttcaggtat tcgcccagac gattaatcac
    12421 atcagcgccc tggatgtatt tacccggtga ttgaataatg cggtccataa ttgctccttt
    12481 agagatgagt agtgccaaat gcggcattcc agtcgtgttc gaacttctct atagctgact
    12541 ctaccgcagg ggtgttgagc atttgttgcg ctacatctaa gggcagggtg atggattcac
    12601 atcctgccag taaacagtcc agcgcctgac gcggcgtttt aaagctggcc gccagcacca
    12661 tgctttctgg tgcgtgcatt tctaacagcg cttgcagctc ctgaaccgta cgaatgccgt
    12721 ccccgccctg ggcatccacg cggttaacat acggagcaac gtattttgcc cctgccagtg
    12781 cggctaataa cccttgtgcg gcgctatata cagcagtgcc aagtgtagta ataccctctt
    12841 ttttcagtat tttaattgct gccagacctt cggaagtcac cgggattttc accacaatgc
    12901 ccggaatggc atcgcgcaga cgcttcgctt cttccaccat cccctgcgcg tcgcggctca
    12961 tggtctgagc aaacagaatg ccctcatcac caatcgcttt ttgcagacgc ggcagcactt
    13021 cccatatgga ctccttgctg gcagcgataa tgctcgggtt agttgtcacc ccggcaatcg
    13081 ggaatatgtg tgccagacgt tcgacttctg cgacgttagc ggtgtccaga tacagttcca
    13141 tgatgtttcc ttttacagtt ccagttcatg ttgcagcagg ctggcgatag cgtcggcaga
    13201 ggcggcgtta accagcgcgt tacggaattc ttcgtgcatg atgcggcgag cgaggcgcga
    13261 gaaaatgcgc atatgctgat cgcccgcagc gtgtttgttc agggttaaca taatgatgaa
    13321 ttgcgcttca tcatcgcccc agcgcaccgg cgcttgcaga cgcgctacgc tgatggtgga
    13381 ttgctcaatg tgttctgatt tgctgtgggg aatggcaaaa ctaaagccca gaccggtaga
    13441 gaaaacggcc tcgcgcgccc acaagtcggc ttccagttta cgcggatagc ggcagcggcc
    13501 cgccagcagc aggttatcgg tcatgccttt gagcacttct tctttgctgc gccagtcgct
    13561 ttccagtgtg atgcactcgg cggtgaccag cggtgcgtct tgttgggtca tgcggaattg
    13621 cgccagcagg tgttccactt ccagcgaagt acggcaggcc attgcctggt tgagcaactt
    13681 gcggcactca cggctatcaa gttgcgccat ccgcgctttc gccgccggaa ttgatggtgc
    13741 gctcatgctg agttcatcca gccctaagcc gaccagcaac ggcagcacgg aacctttcgc
    13801 tcccagctca ccgcacagac caatccattt gccctggcga tgcaccgctt gcacggcgta
    13861 atcgagcgcc cgcaagaatg ccggattcag gctgttgtag tgacgagtaa ccttagcgtt
    13921 atcgcgatcc actgccagca gatattgcgt caggtcatta ctaccaatac taaagaaatc
    13981 aatctcttcg cagcattgat cgatgatgaa catcaccgac ggcacttcca gcatgatgcc
    14041 gagctggatc ttctcatcaa acggaatgtg ttcgttacgc agttgctgtt tggcttccgc
    14101 cagtttttct ttcacccata agatctcttc cattgaggag atcatcggga tcatgatttt
    14161 caggctgccg tgagcagagg cacggaggat cgaccgtagt tgtgtggtga acagcgaggc
    14221 gtattcttca taaatacgca ccgcgcgata accgaggaac gggttagctt ctgccggaat
    14281 gttcagataa tcaacgggtt tgtcaccgcc aatatccata gtgcgcacaa taatgctgcg
    14341 tccgttggcg gactccagcg cttgacaaaa aatgttgtac aactcgcttt cgcccggtgc
    14401 gctggtgcga tccatataga gcatttcagt gcggaacaaa ccgacgcctt ccgcaccatt
    14461 gccgaatgcg gcctgcgctt ccacggagtg agcgatgtta gcggcaattt caatgcggat
    14521 accgtcagcg gtacgggctt gttgggtcag ccagacacgc tgttgctcac gcagggcgtc
    14581 ctgtacgcgg gcttcttgct gataataacg agctacggct tcccctggct caaccacaat
    14641 cgccccggcg ttgccgtcga tataaatcgt ttgatgctgc cacggagtaa gggcatcaat
    14701 atctacacca accagtgtcg gaatgttgaa cgaacgggca agaattacgg tatgtgaggt
    14761 ggtgccgcca cttttcagta acagaccttt gaggtgattt ttatccagtt cgaggaactg
    14821 gctgggggtt agttcatcag ccatacaaat ggcgggctgc gtcagtttgc ccggtgccgg
    14881 gaagcgttgc tcaccgtaga tttgctgaag cagctggaag cagacatcgc gtacgtccag
    14941 ggcacgttct tgcaggtagc tgctgctgga acgggaaaac tcttcacaaa agtgattcgc
    15001 actggcgaca atcgcttcgg cgcagcttaa tccggcgctg acgcctgcca gtaaatgttc
    15061 gtgcagggaa gtatcgccag ccagggatcg gtgcgcttcc agaatcgcgc tggtggcacc
    15121 gtcgctatcc agcagacgaa actcaatgtt tttcagtacc agcgtcaggc cgttttccag
    15181 tgcggattgc tcggcgtcaa cgtctttggc tgcgggaaga ttacccagcg cattgagatc
    15241 taaagaagag atcggcgtca gaatgccgcc tgcgctaccg ctgcacacgg tgcgggcgcg
    15301 gataatttgc ggattcagat tggtcagtga aaccggcagt ggttccagtt cgtcagattt
    15361 aacttccgcc agcggcgcat cgcagtgggg gaattcatcg cgcagccatt ggcttaaacg
    15421 ctggtgcgct tcctgttcgt cggccccgga aatcagtaac tggcagttat cgcccgccag
    15481 cgtgtcggtg ccaatcagcg caagggcgct tttggcgttg cccttgcggt cagtgcgcag
    15541 gttatgccac tcaatttgtg atgaaaaagt attacacagc gtttcaacgt ggcttgccgg
    15601 acgtgcatgt acgccgttag gtagctcaca aataaattcc acaatcaggg ccattgcctc
    15661 tctcccggta cggttttctg tttacaggat aagagcgcac ggcaacggcc tgccatgtga
    15721 caaatctgcc aaaagctgga caaatgtaat gtaaccgtca atttgcgacg cgtctcacaa
    15781 gacgccgttt tgcggcatgc ttccggatta tcgcaagtta tgaggcggat cgcatttttg
    15841 tactgatatt acaaaagtcc agtaaatggc ctttttatcc actgtttgct ccgcctgcga
    15901 ttgcctattg tttcctccat caaaaatatg gacatgggct ttcgcaagcc cgggagcagc
    15961 ttatgaatga gttggtgcag atcctgaaaa atacccgtca gcatttaatg acgggcgttt
    16021 cacacatgat tcccttcgtg gtatcgggcg gtattttgct ggcggtttcc gtcatgttgt
    16081 atggcaaagg cgcagtgccg gatgccgtag ccgatccgaa tctgaaaaaa ctgtttgata
    16141 tcggcgttgc aggtttgacg ctgatggtgc ctttcctcgc cgcttacatc ggttactcca
    16201 ttgcagagcg ttctgcgctg gctccgtgcg ctatcggtgc ctgggttggt aacagctttg
    16261 gtgcgggctt ctttggtgca ctgatcgccg ggattatcgg cggcatcgtg gtgcattacc
    16321 tgaagaaaat tccggtgcat aaagttctgc gctcggtgat gcccatcttc atcattccga
    16381 tcgtcggcac actgattacc gcaggcatca tgatgtgggg tctgggcgag cctgtagggg
    16441 cgttgaccaa cagcctgact cagtggcttc aggggatgca gcagggcagc attgttatgc
    16501 tggcggtgat catgggtctg atgctggcgt tcgatatggg cggtccggtt aacaaagtgg
    16561 cctatgcctt catgctgatt tgcgttgctc agggtgttta taccgtggtg gccattgctg
    16621 ccgtgggtat ttgtatcccg ccgctgggga tggggctggc gacgctgatt ggtcgtaaaa
    16681 atttctccgc agaagagcgc gaaaccggta aagcggcgct ggtgatgggg tgcgttgggg
    16741 ttactgaagg ggcgattcct ttcgccgcag ccgatccgtt acgtgtaatt ccttccatca
    16801 tggttggttc agtttgtggt gcagtaactg cggcgctggt cggtgcgcag tgctatgcag
    16861 gctggggtgg tctgattgtg ctgccggtgg ttgaaggtaa gttgggttat atcgcagcag
    16921 tggctgtcgg ggcggttgtg acggcagtct gcgtgaacgt gctgaaaagt ctggcgcgta
    16981 aaaatggatc ttctactgat gaaaaagaag acgacctgga tttggatttt gaaattaatt
    17041 aattgaggaa cgagccatga cgaaaattat tgcagtaacc gcgtgtccat ctggtgttgc
    17101 ccatacttat atggctgcag aggcgctgga aagtgccgcg aaagcgaaag gctgggaagt
    17161 gaaagtagaa acccagggct cgattggtct ggaaaacgaa ctgactgcgg aagacgtggc
    17221 gagcgccgat atggtgattc tgactaaaga tatcggcatc aagtttgaag agcgttttgc
    17281 tggcaagacc atcgtgcgcg tcaacatcag cgatgcggta aaacgtgctg atgccattat
    17341 gagcaaaatt gaagctcacc tggcgcaaac cgcttaagtt cttctcccgc tcgcaagggc
    17401 gggttcgctt tcccacagga gttcctcatg acgaatcgta tctctcgcct caaaactgca
    17461 ctgtttgcca atacccgtga aatctcgctg gagcgtgcgc tgctttatac cgccagccat
    17521 cggcaaaccg aaggcgaacc ggtgatattg cgccgggcga aagcaacagc gtatatcctt
    17581 gaacatgttg aaatttcgat tcgtgatgaa gaactgattg ccggtaaccg caccgtaaaa
    17641 ccgcgcgccg ggattatgtc gccggaaatg gacccttact ggctgctgaa agagctgggt
    17701 caattcccga cgcgtccgca ggaccgtttt gctatcagcg aagaagataa acgtatctac
    17761 cgcgaagagt tgttcccgta ctgggaaaaa cgttcgatga aagatttcat caacgggcag
    17821 atgacggatg aagtaaaagc cgcgactagc acgcagattt tcagcatcaa ccagacggat
    17881 aaaggccagg ggcacattat tattgattac ccgcgcctgc tgaatcacgg gctgggggag
    17941 ctggtggcac agatgcagca acattgtcag caacagccgg agaatcactt ttatcaggca
    18001 gcgctgttac tgctcgaagc ctcgcagaaa cacattttgc gttacgccga actggcggaa
    18061 acgatggcgg caagctgcac tgatgcccag cgtcgcgaag aactgttgac tattgcggag
    18121 atctcccgcc ataacgccga acataaaccg cagacgttct ggcaggcgtg ccagttattc
    18181 tggtacatga acatcattct gcaatacgaa tccaacgcca gttcgctgtc tttagggcgc
    18241 ttcgaccagt atatgttgcc gttctatcag gcatctttaa cccagggcga agatccggcg
    18301 ttcctgaaag aactgctcga atctttatgg gtgaaatgca acgacattgt gctgttgcgc
    18361 tccaccagca gcgcgcgtta tttcgcaggt ttcccgactg gctataccgc actgctcggc
    18421 gggttaaccg agaacggacg tagcgcggtg aacgtgcttt cgttcctctg cctcgatgcc
    18481 tatcaaagcg tacaattacc gcaaccgaac ctaggggtgc gcactaacgc cttgatcgac
    18541 acgccgttcc tgatgaaaac cgcagaaacc attcgcctcg gcaccggtat tccgcaaatc
    18601 tttaacgatg aagtggtggt gccagcgttc ctcaaccgtg gcgtttcgct ggaagatgcg
    18661 cgcgactatt ccgtagtggg ctgtgtggaa ttatctattc ccggcagaac ctacggcttg
    18721 catgacatcg cgatgtttaa tctgctgaaa gtgatggaaa tctgcctgca tgaaaatgaa
    18781 ggcaatgctg cgctgactta tgaaggttta ctggagcaga tccgcgccaa gatcagccac
    18841 tacatcaccc tgatggttga gggcagcaat atttgtgata tcggccatcg cgactgggca
    18901 cctgtaccgc tgctctcgtc ttttatcagc gattgtctgg aaaaaggtcg cgatattacc
    18961 gatggcggcg cgcgttataa cttctccggc gtacagggaa ttggtatcgc caacctgagc
    19021 gattctctcc atgcgttgaa agggatggtt tttgatcaac agcgtttaag ttttgacgaa
    19081 ttgctgtcgg tattaaaagc caactttgca acgccagaag gcgaaaaagt ccgcgctcgc
    19141 ttaattaacc gctttgagaa atacggtaac gatatcgacg aggtgggtaa cattagcgcc
    19201 gaactgttgc gccactactg caaagaagtg gaaaaatacc agaacccgcg cggcggttac
    19261 ttcacgccgg gatcgtatac cgtttctgct cacgtcccgt tgggatcggt ggttggcgcg
    19321 acgccagacg gtcgttttgc cggagaacag ttggcagacg gcggcttgtc acccatgctg
    19381 ggccaggacg cacaagggcc aacagcggta ctgaagtcag tcagtaagct cgataacacg
    19441 ctgctgtcta acggtacgtt gctgaacgtg aaattcactc cggcgaccct ggaaggtgaa
    19501 gcgggattac gcaaactggc cgacttctta cgggcgttta cccagcttaa gttgcagcat
    19561 attcagttta acgtggtgaa cgccgacacg ttgcgggaag cgcaacagcg cccacaagat
    19621 tatgccgggc tggtggtgcg cgttgccgga tacagcgcct tctttgtcga actgtcgaag
    19681 gagatccagg atgacatcat ccgccggaca gcgcatcagc tgtaacgttg tggaaacgcg
    19741 ccgcaatgat gtggcgcgca ttttcaacat tcagcgttat tcactgaatg acggcgaggg
    19801 cattcgtacg gtggtctttt ttaaaggctg tccgcatctt tgcccgtggt gtgctaatcc
    19861 ggagtcgatc tccggcaaaa tccagacggt acgcagagag gcgaaatgtc tgcactgtgc
    19921 gaaatgtttg cgtgatgcgg atgaatgccc ctccggggcg tttgaacgga ttggtcgcga
    19981 tatcagcctt gacgcgctgg aacgggaagt gatgaaagat gacattttct ttcgcacgtc
    20041 cggcggcggc gtcacgcttt ctggcggcga agtgttaatg caggcggagt ttgctacccg
    20101 ttttttacag cggctgcggt tgtggggcgt gtcatgcgcc attgaaactg ccggagacgc
    20161 gccagccagc aagctgttac cgctggcgaa attgtgcgat gaagtgttgt tcgatttaaa
    20221 aattatggac gcgactcagg cgcgggatgt ggtgaagatg aacctgccac gcgtgctgga
    20281 gaatctgcgt ttgctggtga gtgagggcgt caacgtgatc ccgcgtttac cgctgatccc
    20341 tggtttcacg ctcagccggg agaatatgca gcaggcgctg gatgtactga tcccgctgaa
    20401 aatcaggcag atccatctgt taccgtttca tcagtacggc gaaccgaaat accgcctgct
    20461 ggggaaaaca tggtcgatga aagaggtgcc tgcgccgtcg tcagccgatg tgacaacgat
    20521 gcgcgaaatg gcagaacggg ccggatttca ggttaccgtg ggaggttaaa atggcatacc
    20581 tggtggcagt aaccgcctgc gtaagcggcg tggcgcatac atatatggcg gcggaacggc
    20641 tggaaaagtt gtgcctgtta gagaagtggg gagtcagcat tgaaactcag ggcgcgctgg
    20701 gaacggagaa tcgtttagcg gacgaggata tccgtcgggc ggatgttgct ctgttgatta
    20761 cggatatcga gcttgccggt gccgagcgat ttgaacattg ccgctatgtg caatgcagca
    20821 tctacgcatt cctgcgtgag ccgcagcagg taatgagcgc ggtgcgcaaa gtgctttctg
    20881 cgccgcagca aacccatctt attctggagt agtcggtttt tctgtcagtt ggctgtggta
    20941 ctgccggcga tattccgacg gcgagcgttc ggtgttttta cgaaacagac ggcagaagta
    21001 gttgctgtcg acaaagccgc aggcgtgcgc cacttctttt accttcaggt catagccttt
    21061 caataacgtc ttagcgtgct ccagtcgcgt gtgattcagg tattcgttaa aaccaatggc
    21121 ccccgttttt tgaaacaggt gcgagagata gtttggtgaa atatagaatg cctgtgcgac
    21181 agattcgcgg gtaagcgcgg aggcatagcg ttcgtcgata taatcgcgaa tagcttcgaa
    21241 tagtgcctgg ctgcgtgagg cggtctggat ttggctgcca agcaaatcgc ggcagtggct
    21301 aagcaggctg gcgacgataa gccttgccgt ttgctgctcc tgcggctgca tttgcatttc
    21361 attgagcgtt tgtagcagaa aagaacctat gcgtgggccg cgtcgggcga cgtgttgctt
    21421 cgccagattt tgatattgtt tgccatccca ttgcacgacg ctgaagccga gttgctgttt
    21481 gccaaacagc acgctaaagg tagtagcagg ggcttgccat tgtggaaaat tccagccacc
    21541 agcggcaacg tacagcacat cgccgggaac taacgttgct ccagcgccgg tatcaacaaa
    21601 ctcaccttcc agcacaattt ccagccgtgg aaaatcgacc tgatacgcaa gatcgggcac
    21661 cgggccgtta ctgctggcaa agtaaatctg gcgcagggac agcgggccgt tgatcaggcg
    21721 ggagagcaga tagctgacgt cgtgatacat gttattccct taggtatgga gacgggaaaa
    21781 cagtaactgg cattattaaa aaaggccacc tgaaaagtgt ggcctgaggg gagttcgatg
    21841 taaacattgc ggcagttgta ttgccggatg tggcgtaaac gccttatcca gtctaccgcc
    21901 gcgatctgta ggccggataa gacgcgtcaa gcgtcgcatc cggcaaatag cgcctggctg
    21961 ataattactg attacctacc tggtcgccat acggcagcgt gtcgtaatcg atcagcgcgt
    22021 ttttcttgta cgggttacca atccagcggg tattttcttt gaactgcgga ttcaccactg
    22081 aacgggttgg atcgtaaccg tcgtaagata agcccgctaa atctgaccag gtgtggatca
    22141 gttccgcaag gctgtattta cgatcaacat cctgcgagaa atcacgcgga tgagtcgctt
    22201 gccatttttc cgacgtccac agcaggaacg gaatggtgta catatgacgc gtcgggttgt
    22261 cctcattacg cccctgggtt ttatgaggcg gcgtgtcgta aacctcttca ccgtggtcag
    22321 agaagtaaac caggaaaccg ttcgggtctg ctgctttaaa gtctttaatc aggctggcaa
    22381 ccacatgatc gttatacagg ttagcgttgt cataatcgtt atatgactcc agctcttccg
    22441 cgtttaatcc cggcggaaca tgatcggtat tgccatcaaa cttgccctga ttttccgggt
    22501 agcggtattt gtatttgata tgcgtaccca gcagatgaac gatgatcagt ttcttcggcg
    22561 cagggtcctt cagcacttcc tggaacggct tcagcacgtt agtgtcgtat tcacgcgcac
    22621 tctgcgtacg ttgctggttc atgtagtact gcttgtcggt ctggcgcgaa aataccgtca
    22681 gcatggtatt gcgggcggtc atcgtctgct ggttggtgat ccagaaggtt ttataacccg
    22741 cctgtttcat catgttcatc agcgacggct gcgtcagata cagatccggg ttcttttcat
    22801 tggcaaaggt cagcgcctgt tgcaggattt caatggtgta cggacgagac gtaactacgt
    22861 tattaaacac ggtcagattc ggatcggttt tatgcagcgc attcagctcc ggcgtggttt
    22921 cacgcggata accgtacaga ctcatgcgtc cacgctgggt tgactcgcca atcaccagca
    22981 ctaaggtgcg cggttcgtta cccgattcat ctttgaaatt agccagcggc ggcaaggcat
    23041 tattttcatt cagtagcttt gttagcgagt ttagttgctg acgatactga taatagccgg
    23101 tcaggaattg ccacggtgcg gcaggctcca tacgcgaggc caggttatcc aacgttttct
    23161 caaacggctt gtttttgata aacatattca tggcgatcgg atgcagaatc aagccataaa
    23221 gcagggcaaa agagacaaca taacgccacg gctttggaat atagaccggg cgcaggcgtg
    23281 tccacagcag aactgccacc gccgtatagg ccagcgcgat aagcacaatt ttcaggctga
    23341 aatactggct taaatactcg ctggcttcgt tggtgttggt ttcgaacatc acaaacagaa
    23401 cgctctgcga gaactcctga ccgtagatga cgtagtagca cagcgccgcc agagaggccg
    23461 cccatagcac cacgccgatc actgcggcaa taattttgat ccgcttcgga aagaggaata
    23521 ccggaattaa ccacagcgag ctgaataaca gcgagtcgcg aatgccgttg gtgccactat
    23581 aaccactgat gtaaataatg gcctgtagca gagtagagaa aaaccaaaag tagagcaatg
    23641 cccaacccag ggctttccag ctaaaaagag gtttagcctg gacttctgtg gaatgcatag
    23701 taagaacctg tcttgaaaaa aatatcgccg aatttaccga caattcctta aggatatctg
    23761 aagggatatc cagaatttga ataaaatgca gacagaaata tattgaaaac gagggtgtta
    23821 gaacagaagt atttcagaaa accctcgcgc aaaagcacga gggtttgcag gagaggaaga
    23881 ttagccggta ttacgcatac ctgccgcaat cccggcaata gtgaccatta acgcctgctc
    23941 gacgcgagga tccggttcct ggccttcttt ttctgcctgg cgggagcggt gcagcaactc
    24001 ggcttgcaat acgttcagcg ggtcggtgta aatattccgt agctgaatag actctgcaat
    24061 ccacggcaga tcggccatca gatgggaatc gttggcaatc gccagcacca ctttgatgtc
    24121 ttcttcttgc agattgcgta actctttacc taacggccac agtgttttgt ctaccaggcg
    24181 ttggtcatag tattccgcca gccacaggtc tgctttggcg aagaccatct ccagcatgcc
    24241 gagacgcgtc gagaagaatg gccaatcgcg gcacatagcc tccagctcgc tctgtttgcc
    24301 atcttcgacc actttttgca gcgccgtacc tgcacccagc caggcgggga gcatcagacg
    24361 gttttgcgtc caggcgaaga tccacggaat ggcgcgtagt gactcgacgc cgccggttgg
    24421 gcgacgtttc gccggacgtg aacccaacgg cagtttgccc agttcctgtt ccggcgtagc
    24481 ggagcggaag taaggcacaa aatctttgtt ttcacgtacg tagccacggt agagatcgca
    24541 ggagatgact gatagttcat ccataatgcg acgccagctc tctttcggct caggtggtgg
    24601 cagcaggttg gcttccagta tcgccccggt gtaaagcgac aggctgctga cggtgatttc
    24661 tggcagacca tatttaaagc ggatcatctc gccctgttca gtcacgcgca ggccaccttt
    24721 caggcttcct ggcggttgtg acagcagcgc cgcatgagca ggtgcgccgc cgcgaccaat
    24781 ggaaccgccg cgaccgtgga acaacgtcag ctcaataccc gctttttcgc aggttttgat
    24841 taatgcatcc tgtgcctgat attgcgccca ggacgctgcc atcacgcccg catcttttgc
    24901 tgagtcggaa tagccaatca tcaccatctg tttgccctga atcaggccgc gataccagtc
    24961 gatattcagc aactgagtca tgacatcgtt ggcgttgttc aggtcatcga gagtttcaaa
    25021 cagcggagca accggcatcg caaacccgat acccgcttct ttcagcagca ggtggacagc
    25081 cagcacgtcg gacggcgttt tcgccatcga gatcacgtag gcggcaatgg agccttgcgg
    25141 tgcttcggca atcacctggc aggtatcgag cacttcgcgc gtttcggcgc ttggttgcca
    25201 gttgcgcggc agaagcggac gtttggagtt cagttcgcgg atcaggaacg cctgtttgtc
    25261 ggcctctgac cagctttcgt agtcgccgat accgaggtag cgggtcagct cgcccagcgc
    25321 ttcggtatga cgcgtgctct cctgacggat atcaatacgg accagcggta cgccgaaaca
    25381 tttcacgcgg cgcagggtgt cgagcagatc gccgttggcg ataataccca tgccacacgc
    25441 ctgaagtgac tggtagcaag cgtagagcgg ttcccacagt tcttcgtttt gtgtcagcag
    25501 gccttctggt tttggcagtt cttcgccttt caggcgcgct tccagccatg cctgtgtcgc
    25561 catcaggcga gaacgcaggt ttttcatcag atagcgatac ggttctgcgg caccttcttc
    25621 gccaaccagc gccagcagtt caggggtcgc ttcaaccatc gacagttcag aaaccagaac
    25681 ctgaatatct ttcaggaaca gatcggtggc tttccagcgg ctgagcagca ggacgtggcg
    25741 ggtgatatcg gcagtgacgt tcgggttgcc gtcgcggtca ccgcccatcc acgaggtaaa
    25801 acgtaccgga acaaattcga cgggcagttt gtagccgagg ttctcttcca gttgttcgtt
    25861 cagttcgcgc aggtaattcg gtacgccttg ccacaggctg ttttctacta cggcaaagcc
    25921 ccatttggct tcatctaccg ggcttggacg cagcttacgg atttcatcgg tatgccatga
    25981 ctgggcgatc aactggcgca ggcgacgcat cagctggttg tgttcgtagt cagcgatatc
    26041 tttgttatcg agctgtttta agcaggcgtt cacttccacc attttgtgga tcagtgtacg
    26101 acgggtaatt tcggttgggt gagccgtgag gaccagttcc agcgacagcg attccactgc
    26161 ttttttgatg gtgtcttcgc tcagttccgg ctggtttttc agtttacgca gggtgcgggc
    26221 gatcacttcc gggttgctgg cagcttcgcc tttcggcgaa atgctgtggt attgctcggc
    26281 ggtgttggcc aggttcagga actgactaaa cgcacgcgca acgggcagca gctcgtcgtt
    26341 cgacaaattt tgtaaggtgg tgagtaactc ctggcggtta gcatcattgc cagcgcgtga
    26401 agatttggac aacttacgga tagtttctac gcgttcaaga atgtgttctc ccaacgcatc
    26461 cttgatggtt tctcccagca ctttgccgag catactgaca ttactacgca atgcggaata
    26521 ttgttcgttc atattacccc agacacccca tcttatcgtt tgatagccct gtatccttca
    26581 cgtcgcattg gcgcgaatat gctcgggctt tgcttttcgt cgtcttttat aaagccacgt
    26641 aaaagcggtg acgtcaaatg ctgcgaaatc gcttcagcaa acgaataaat agcaggaatt
    26701 tacgtcatta aattcacgac gctttaaata agcgtaactt atgggaatgt taaaaaatcg
    26761 cctcaagtaa caccaaaggt gtaggtcgga taagatgtgc aagcatcgca tccgacatta
    26821 ttgcggcact ggagtttggc aacagtgccg gatgcggcgc aagcgcctta ttcggcctac
    26881 gttttaatgc cagcaaaaat ggtgaatgac ctgggttatc agttcgcggg tgggcttgat
    26941 aaaccgtgtt tccagatatt catcaggctg atgagcctga ttaattgagc caggccccag
    27001 aaccagcgtc gggcataacg tttgaataaa cggcgcttcg gtacagtaat tcaccacttc
    27061 ggtttttgct ccgagcaatt tctcaaccac ttcaaccagt tgatgattcg gtgggcattc
    27121 atagccaggg atcggcggat gcagctcgtc gaccgtcaga cgacccggcc agcgttcgct
    27181 caccggagcc aatgcatcgt tgagcaaacc attaagttca ttgagtgtca tgccaggcag
    27241 cggacgaata tccatatgca actcacacca agcgcaaata cggttagaag cgtcgccacc
    27301 gtgaatatgc ccgaggttga gcgtagggta tggcacggta aacgcttcgt agtgataacg
    27361 ttctttcagg ttatcgcgca attgcaaaat atgcccgatg gcgtcgtgca ttagttctat
    27421 agcgttaact ccgcgtgctg gatcgctgga gtgccccgac tggccctgaa tacggatggc
    27481 gttagagata tgacctttat gtgcgcgtac cggttgtagt gacgtcggtt cgccaatgat
    27541 ggcgcaatcc gggcgcaggg cggtagtttc ggcaaaataa cgcgctccgg ccatactggt
    27601 ttcttcatca gcagtcgcca gaatgtagag cggttttttc agtttcgtga cgtcgacatc
    27661 gcgtagcgca tcaaggataa acgcaaaaaa gcctttcatg tcggcggtgc ctaagccgta
    27721 aagcttgccg tcatgctccg tcagtgtaaa cggatcgcgc gtccagcgac cgtcatcaaa
    27781 tggcaccgta tcggtatgcc ccgccagcaa caagccgcca gccccctgtc cggtacttgc
    27841 cagcatatta aatttgttgc gagttcctgg tacaggctgc acttccacat tgaagcctaa
    27901 atctttaaac cagtccgcca gcagagtgat taaatctgca ttgctttgat cgagtgcctc
    27961 ttccgtggcg cttattgaag gtgtggcaat cagagcacgg taaatctcga taaatggcgg
    28021 taatttgttt ttcattgttg acacacctct ggtcatgata gtatcaatat tcatgcagta
    28081 tttatgaata aaaatacact aacgttgagc gtaataaaac ccaccagccg taaggtgaat
    28141 gttttacgtt taacctggca accagacata agaaggtgaa tagccccgat gttgaatacg
    28201 ctgattgtgg gtgccagcgg ctacgctggc gcagagctag tgacctgtgt aaatcgccat
    28261 ccgcatatga acataaccgc tttgactgtt tcagcgcaaa gcaatgatgc gggaaagtta
    28321 atctccgatt tgcatccgca gctaaaaggc atcgttgatc tgccgttgca gccgatgtcg
    28381 gatatcagcg agtttagccc tggggtggac gtggtgtttc tcgccaccgc ccatgaagtt
    28441 agccacgatt tagcgccgca gtttcttgac gcgggctgcg tggtgttcga cctttccggc
    28501 gcgtttcgtg ttaacgacgc caccttctat gaaaaatatt acggctttac ccatcaatac
    28561 ccggaactgc tggaacaggc agcctacggt ctggcggagt ggtgcggtaa taaattaaaa
    28621 gaagcgaatt tgattgcggt gccaggctgt tatccgacgg cagcacagtt ggcgctgaaa
    28681 ccgttgattg atgccgatct tcttgacctc aatcagtggc cggtgatcaa cgccaccagc
    28741 ggcgtgagcg gtgcaggacg taaggcagcg atttcaaata gtttttgtga agttagcctg
    28801 caaccgtatg gcgtctttac ccatcgccat caaccagaga tcgccacaca cctcggtgct
    28861 gacgttatct tcaccccgca tctgggcaat ttcccgcgcg gcattctcga aaccattacc
    28921 tgccgcctga aaccgggtgt gacccaggcg caagtggcgc aagcgttaca gcaggcgtat
    28981 gcccataaac cgctggtgcg gctgtatgac aaaggcgttc cggcgctgaa aaatgtcgtt
    29041 gggctaccat tttgcgatat cgggtttgcc gttcagggcg aacatctgat tattgtggcg
    29101 accgaagaca acttactgaa aggcgcggcg gcacaagcgg tacagtgcgc caatattcgt
    29161 ttcggctatg cggaaacgca gtctcttatt taagggtgca atgatgaatc cattaattat
    29221 caaactgggt ggcgtactgc tggatagtga agaggcgctg gaacgtctgt ttagcgcact
    29281 ggtgaattat cgtgagtcac atcagcgtcc gctggtgatt gtgcacggcg gcggttgcgt
    29341 ggtggatgag ctgatgaaag ggctgaatct gccggtgaaa aagaaaaatg gcctgcgggt
    29401 gacgcctgct gatcagattg acattatcac cggagcactg gcgggaacgg caaataaaac
    29461 cctgttggca tgggcgaaga aacatcagat tgcggccgta ggtttgtttc tcggtgacgg
    29521 cgacagcgtc aaagtgaccc agcttgatga agagttaggt catgttggac tggcgcagcc
    29581 aggttcgcct aagcttatca actccttgct ggagaacggt tatctgccgg tggtcagctc
    29641 cattggcgta acagacgaag ggcaactgat gaacgtcaat gccgaccagg cggcaacggc
    29701 gctggcggca acgctgggcg cggatctgat tttgctctcc gacgtcagtg gcattctcga
    29761 cggcaaaggg caacgcattg ccgaaatgac cgccgcgaaa gcagaacaac tgattgagca
    29821 gggcattatt actgacggca tgatagtgaa agtgaacgcg gcgctggatg ctgcgcgcac
    29881 gttgggccgt ccggtagata tcgcctcctg gcgtcatgcg gagcagcttc cggcactgtt
    29941 taacggtatg ccgatgggta cgcggatttt agcttaagtt ttgttggccg gaggcgcagc
    30001 tttccggcat tgaatttcaa aataaggaaa cagagttatg gcactttggg gcgggcgttt
    30061 tacccaggca gcagatcaac ggttcaaaca attcaacgac tcactgcgct ttgattaccg
    30121 tctggcggag caggatattg ttggctctgt ggcctggtcc aaagccctgg tcacggtcgg
    30181 cgtgttaacc gcagaagagc aggcgcaact ggaagaggcg ctgaacgtgc tgctggaaga
    30241 tgttcgcgcc aggccacaac aaatccttga aagcgacgcc gaagatatcc atagctgggt
    30301 ggaaggcaaa ctgatcgaca aagtgggcca gttaggcaaa aagctgcata ccgggcgtag
    30361 tcgtaatgat caggtagcga ctgacctgaa actgtggtgc aaagataccg ttagtgagtt
    30421 actgacggct aaccggcagc tgcaatcggc gctggtggaa accgcacaaa acaatcagga
    30481 cgcggtcatg ccaggttaca ctcacctgca acgcgcccag ccggtgacgt tcgcgcactg
    30541 gtgcctggcc tatgttgaga tgctggcgcg tgatgaaagc cgtttgcagg atgcgcttaa
    30601 gcgtctggat gtcagcccgt taggttgtgg cgcgctggcg ggaacggctt atgaaatcga
    30661 ccgtgaacag ttagcgggct ggctgggatt tgcttcagca acccgtaaca gtctcgacag
    30721 cgtttctgac cgtgaccacg tgctggaact gctttctgct gccgctatcg gcatggtgca
    30781 tctgtcgcgt tttgctgaag atctgatttt ctttaacacc ggcgaagcgg ggtttgtgga
    30841 gctttctgac cgcgtgactt ccggttcatc attaatgccg cagaagaaaa acccggatgc
    30901 gctggagctg attcgcggta aatgcggtcg ggtgcagggc gcgttaaccg gcatgatgat
    30961 gacgctgaaa ggtttgccgc tggcttacaa caaagatatg caggaagaca aagaaggtct
    31021 gttcgacgcg ctcgatacct ggttggactg cctgcatatg gcggcgctgg tgctggacgg
    31081 cattcaggtg aaacgcccgc gttgccagga agcggcgcaa cagggttacg cgaacgccac
    31141 tgaactggcg gattatctgg tggcgaaagg tgtgccgttc cgcgaggcac accatattgt
    31201 gggtgaagct gtggtggaag ccattcgtca gggcaaaccg ctggaggagc tgccgctcac
    31261 cgagttgcag aaatttagtc cggtgattgg tgaagatgtc tatccgattc tgtcgctgca
    31321 atcgtgcctc gacaagcgtg cggcaaaagg cggcgtctca ccacagcagg tggcgcaggc
    31381 gattgctttt gcaagggcgc ggttggggta agaacattta tatgtacaaa tttgagcctg
    31441 gcttatcgcc gggctttttt atggcaaaaa aaagcggatc ctggagatcc gcaaaagttc
    31501 acgttggctt tagttattcg agttgagaaa ctctcgaaac gggcagtgac ttcaagggtt
    31561 aaaagaggtg ccgctccgtt tctgtgagca attatcagtc agaatgcttg atagggataa
    31621 tcgttcattg ctattctacc tatcgccatg aactatcgtg gcgatggagg atggataatg
    31681 aatattcgtg atcttgagta cctggtggca ttggctgaac accgccattt tcggcgtgcg
    31741 gcagattcct gccacgttag ccagccgacg cttagcgggc aaattcgtaa gctggaagat
    31801 gagctgggcg tgatgttgct ggagcggacc agccgtaaag tgttgttcac ccaggcggga
    31861 atgctgctgg tggatcaggc gcgtaccgtg ctgcgtgagg tgaaagtcct taaagagatg
    31921 gcaagccagc agggcgagac gatgtccgga ccgctgcata ttggtttgat tcccacggtt
    31981 ggaccgtacc tgctaccgca tattatcccg atgctgcacc agacctttcc aaagctggaa
    32041 atgtatctgc atgaagcaca gacccaccag ttactggcgc aactggacag cggcaaactc
    32101 gattgcgtga tcctcgcgct ggtgaaagag agcgaagcat tcattgaagt gccgttgttt
    32161 gatgagccaa tgttgctggc tatctatgaa gaccacccgt gggcgaaccg cgaatgcgta
    32221 ccgatggccg atctggcagg ggaaaaactg ctgatgctgg aagatggtca ctgtttgcgc
    32281 gatcaggcaa tgggtttttg ctttgaagcc ggggcggatg aagatacaca cttccgcgcg
    32341 accagcctgg agacgctgcg caacatggtg gcggcaggta gcgggatcac tttactgcca
    32401 gcgctggctg tgccgccgga gcgcaaacgc gatggggttg tttatctgcc gtgcattaag
    32461 ccggaaccac gccgcactat tggcctggtt tatcgtcctg gctcaccgct gcgcagccgc
    32521 tatgagcagc tggcagaggc catccgcgca agaatggatg gccatttcga taaagtgtta
    32581 aaacaggcgg tttaaaccgt ttaacgcagc tacccgatag gcttccgcca tcgtcgggta
    32641 gttaaaggtg gtgttgacga agtactcaat agtgttgccg ccacctttct gttccataat
    32701 cgcctgaccg atatgaataa tttcggcagc gcgctcgcca aagcagtgaa tgcccagaat
    32761 ctcttttgtt tcccgatgga acaaaatttt cagcgtgccc acgttcatgc cgacgatttg
    32821 tgcacgtgcc agatgtttga actgggcgcg gcccacttca tatggcactt tcatcgcggt
    32881 cagctgctgt tcggttttgc ccacagagct gatttccggg atggtgtaaa tgccggtagg
    32941 gatatcttca atcagatgcg cggtggcttc gccttttacc agcgcctgcg cggcaatgcg
    33001 cccctggtca taggccgctg atgccaggct cggataacca atcacgtcgc ccaccgcgta
    33061 aacgtgcggc tgtgcggtct gatacatgct gttgaccttc agctgtccgc gactgtcagt
    33121 ttccagccca atgttctgta acgccagcga atcggtatta ccggtacgac cgttggcata
    33181 gagcaggcag tcagctttca gttttttacc cgacttcaga tgcatgatca caccgtcgtc
    33241 acagccttcg atcttctcgt actcttcgtt gtgacgaatc actacgccac tgttccagaa
    33301 gtgataggag agagaatctg acatctcttg atcgagaaat gccagcaggc gatcgcgggt
    33361 gttgatcaga tccactttta catccatacc gcggaagatc gacgcatatt cacagccgat
    33421 cactccagca ccatagataa gtacatggcg cggttcgtgg tgcatgctga gaattgagtc
    33481 gctgtcgtaa atgcgtggat gggtgaaatc aacatctgtt ggatgatatg gacgagagcc
    33541 gcaggcaata acaaattttt cagcggttag tgtttcaacg ctgccgtccg ggcaatccag
    33601 cgccaacgta tgctcgtcaa caaagcgagc gttcccctgc aatatttcac agtgattacg
    33661 ttcgtaaaat ccctgacgca tgcgcgtttg ttgattaatc acgttatcgg catggttaag
    33721 gatatcggca aaagaagagc ggagcagtcg ggaatggtcg ctgtatagtg ggttttgatt
    33781 gaattctata atgcggctga cggcgtgacg gagagctttc gacgggatgg tgccccagtg
    33841 ggtgcaaccg ccgccaacat tttgataacg ctcgataact gcgacgcgcg caccttgctt
    33901 aaccaggccc attgcagcgc cttcgccgcc ggggccggaa cctattacta tggcatcgta
    33961 atcgtaggaa tgtggcatgg tagggcttac ctgttcttat acataaaagc aacagaatgg
    34021 taacatttta tcgcgggtaa gccaattgat ccccgtcatt aatctggcta tagcgtgagc
    34081 ggcctttgct ttgtctgcct ccttcttaac ccatttattc actattaatc gctttttatt
    34141 atggctgata gttttatctt gccgaagcgt gcttttttgt tattgtcttt gtgaaatctt
    34201 cttataaaac aaa
//
LOCUS       DBFIFM010000029        33774 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.029, whole genome shotgun sequence.
ACCESSION   DBFIFM010000029 DBFIFM010000000
VERSION     DBFIFM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.029"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..569
                     /gene="yraH"
                     /locus_tag="N7C74_001237"
     CDS             <1..569
                     /gene="yraH"
                     /locus_tag="N7C74_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2431138.1"
                     /translation="TAIAGLLALFAGNAAATDGEIVFDGEILKSACEINDSDKKIEVA
                     LGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPN
                     FPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSAGIVNVDLI
                     AYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            649..1344
                     /gene="yraI"
                     /locus_tag="N7C74_001238"
     CDS             649..1344
                     /gene="yraI"
                     /locus_tag="N7C74_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU2431139.1"
                     /translation="MSKRTFAMIFTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLIRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVSDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKADLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1373..3889
                     /gene="yraJ"
                     /locus_tag="N7C74_001239"
     CDS             1373..3889
                     /gene="yraJ"
                     /locus_tag="N7C74_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU2431140.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYNFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSVNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3900..4633
                     /locus_tag="N7C74_001240"
                     /pseudo
     CDS             3900..4633
                     /locus_tag="N7C74_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(4676..5536)
                     /gene="rsmI"
                     /locus_tag="N7C74_001241"
     CDS             complement(4676..5536)
                     /gene="rsmI"
                     /locus_tag="N7C74_001241"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HGU2431141.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            5601..7637
                     /gene="lpoA"
                     /locus_tag="N7C74_001242"
     CDS             5601..7637
                     /gene="lpoA"
                     /locus_tag="N7C74_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HGU2431142.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFVGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            7595..7990
                     /gene="yraN"
                     /locus_tag="N7C74_001243"
     CDS             7595..7990
                     /gene="yraN"
                     /locus_tag="N7C74_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HGU2431143.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDTFNDHS"
     gene            8010..8600
                     /gene="diaA"
                     /locus_tag="N7C74_001244"
     CDS             8010..8600
                     /gene="diaA"
                     /locus_tag="N7C74_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HGU2431144.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            8610..9185
                     /gene="dolP"
                     /locus_tag="N7C74_001245"
     CDS             8610..9185
                     /gene="dolP"
                     /locus_tag="N7C74_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HGU2431145.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9299..10339)
                     /gene="yraQ"
                     /locus_tag="N7C74_001246"
     CDS             complement(9299..10339)
                     /gene="yraQ"
                     /locus_tag="N7C74_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGU2431146.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10412..11047)
                     /gene="yraR"
                     /locus_tag="N7C74_001247"
     CDS             complement(10412..11047)
                     /gene="yraR"
                     /locus_tag="N7C74_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HGU2431147.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            11175..11693
                     /gene="yhbO"
                     /locus_tag="N7C74_001248"
     CDS             11175..11693
                     /gene="yhbO"
                     /locus_tag="N7C74_001248"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HGU2431148.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(11673..12116)
                     /gene="yhbP"
                     /locus_tag="N7C74_001249"
     CDS             complement(11673..12116)
                     /gene="yhbP"
                     /locus_tag="N7C74_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HGU2431149.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDVQKVAFY
                     ILTEEKTRHAQMRGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRPDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            12167..12469
                     /gene="yhbQ"
                     /locus_tag="N7C74_001250"
     CDS             12167..12469
                     /gene="yhbQ"
                     /locus_tag="N7C74_001250"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HGU2431150.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAKLLSSLQTPEIKSD"
     gene            complement(12456..12959)
                     /gene="yhbS"
                     /locus_tag="N7C74_001251"
     CDS             complement(12456..12959)
                     /gene="yhbS"
                     /locus_tag="N7C74_001251"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU2431151.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(12953..13477)
                     /gene="ubiT"
                     /locus_tag="N7C74_001252"
     CDS             complement(12953..13477)
                     /gene="ubiT"
                     /locus_tag="N7C74_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HGU2431152.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            13686..14681
                     /gene="ubiU"
                     /locus_tag="N7C74_001253"
     CDS             13686..14681
                     /gene="ubiU"
                     /locus_tag="N7C74_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HGU2431153.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            14690..15568
                     /gene="ubiV"
                     /locus_tag="N7C74_001254"
     CDS             14690..15568
                     /gene="ubiV"
                     /locus_tag="N7C74_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HGU2431154.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            15649..16656
                     /gene="yhbW"
                     /locus_tag="N7C74_001255"
     CDS             15649..16656
                     /gene="yhbW"
                     /locus_tag="N7C74_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HGU2431155.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSARDAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVAWFDARDPNPHVRPVPG
                     YGEQIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(16773..18017)
                     /gene="mtr"
                     /locus_tag="N7C74_001256"
     CDS             complement(16773..18017)
                     /gene="mtr"
                     /locus_tag="N7C74_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HGU2431156.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIDIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18171..20015)
                     /gene="deaD"
                     /locus_tag="N7C74_001257"
     CDS             complement(18171..20015)
                     /gene="deaD"
                     /locus_tag="N7C74_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HGU2431157.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERRGDGRRFSGERREGRAPRRDDSTGRRRFGGDA"
     gene            complement(20008..20088)
                     /gene="yrbN"
                     /locus_tag="N7C74_001258"
     CDS             complement(20008..20088)
                     /gene="yrbN"
                     /locus_tag="N7C74_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HGU2431158.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20195..21079)
                     /gene="nlpI"
                     /locus_tag="N7C74_001259"
     CDS             complement(20195..21079)
                     /gene="nlpI"
                     /locus_tag="N7C74_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HGU2431159.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21188..23323)
                     /gene="pnp"
                     /locus_tag="N7C74_001260"
     CDS             complement(21188..23323)
                     /gene="pnp"
                     /locus_tag="N7C74_001260"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HGU2431160.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(23570..23839)
                     /gene="rpsO"
                     /locus_tag="N7C74_001261"
     CDS             complement(23570..23839)
                     /gene="rpsO"
                     /locus_tag="N7C74_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HGU2431161.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTRLIERLGLRR"
     gene            complement(23988..24932)
                     /gene="truB"
                     /locus_tag="N7C74_001262"
     CDS             complement(23988..24932)
                     /gene="truB"
                     /locus_tag="N7C74_001262"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HGU2431162.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDVDGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(24932..25333)
                     /gene="rbfA"
                     /locus_tag="N7C74_001263"
     CDS             complement(24932..25333)
                     /gene="rbfA"
                     /locus_tag="N7C74_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HGU2431163.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(25498..28170)
                     /gene="infB"
                     /locus_tag="N7C74_001264"
     CDS             complement(25498..28170)
                     /gene="infB"
                     /locus_tag="N7C74_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HGU2431164.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28195..29682)
                     /gene="nusA"
                     /locus_tag="N7C74_001265"
     CDS             complement(28195..29682)
                     /gene="nusA"
                     /locus_tag="N7C74_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HGU2431165.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQVQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(29710..30162)
                     /gene="rimP"
                     /locus_tag="N7C74_001266"
     CDS             complement(29710..30162)
                     /gene="rimP"
                     /locus_tag="N7C74_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HGU2431166.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30369..30445)
                     /locus_tag="N7C74_001267"
     tRNA            complement(30369..30445)
                     /locus_tag="N7C74_001267"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30409..30411),aa:Met,seq:cat)
     gene            30793..32136
                     /gene="argG"
                     /locus_tag="N7C74_001268"
     CDS             30793..32136
                     /gene="argG"
                     /locus_tag="N7C74_001268"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HGU2431167.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQMENLENKGQ"
     gene            complement(32144..33769)
                     /gene="yhbX"
                     /locus_tag="N7C74_001269"
     CDS             complement(32144..33769)
                     /gene="yhbX"
                     /locus_tag="N7C74_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGU2431168.1"
                     /translation="MTVFNKFARSFKSHWLLYLSVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVAKMLGMYSPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKNAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQSQTLEEVIVHYKGDS
                     LVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
BASE COUNT         8337 a         9057 c         8362 g         8018 t
ORIGIN      
        1 aaacagctat tgcgggctta cttgcgcttt tcgcaggtaa tgccgctgca accgatggtg
       61 aaatagtttt tgatggcgag attttaaaat ccgcttgtga aatcaatgac tctgataaga
      121 aaattgaagt tgctcttggt cactataatg ccgaacagtt tcgttctgta ggcgatcgta
      181 gcccaaaaat tccattcact attcctttag ttaactgccc agtgactggg tgggagcacg
      241 ataacggcaa cgttgaagcg tctttccgtt tatggctgga aacgcgggat aatggtaccg
      301 tccctaactt ccctaacttg gcaaaagtgg gctcttttgc tggcacagca gcaacaggtg
      361 tgggtatccg tattgacgat gcggaaagcg gaaaccttat gccactaaat gctatgggca
      421 atgataacac agtttatcag atccctgcgg actccgctgg aattgtcaat gttgacctca
      481 tcgcttacta cgtatcaacc gttgaagcat ccgaaatcac cccaggggaa gcagacgctg
      541 tcgttaacgt aacgctggat taccgttaat acgttacggc gttatctgac ctttcagata
      601 acgccctttt ccttcctatt tctcgttgta tcaggttgaa aaataactat gtcaaaacga
      661 acattcgcga tgatatttac cttgttgtgt agcttctgta tcggccaggc gcttgcaggg
      721 ggtatcgttt tacagcgaac gcgagtgatc tatgatgcca gccgcaaaga ggctgcgtta
      781 cctgtcgcaa acaaaggcgc agaaacacct tatttactgc aatcatgggt agataatata
      841 gatggtacaa gccgtgcccc atttattata accccaccgc taattcgtct tgaagctggc
      901 gatgattcat cactgcgaat tattaaaaca gctgataacc tgcctgaaaa taaagagtcg
      961 ctgttctaca ttaatgttcg tgccattcca gcaaagaaaa aatcagatga tgttaatgct
     1021 aacgagttga cgctggtatt taaaacacgg atcaaaatgt tttatcgccc cgcacacctg
     1081 aagggacggg taagcgatgc gtggaaatca ctggaattta aacgtagtga tcattcactc
     1141 aatatatata acccaactga atattacgtc gtatttgccg gactagcagt cgataaagcc
     1201 gatctcacaa gcaaaattga atatatcgcg cccggagaac ataaacagtt accacttcca
     1261 gcatctggcg gaaagaacgt gaaatgggct gcgatcaatg attatggcgg cagttccggg
     1321 acagaaactc gtccactgca ataacaaata taaaaacaca ggtcatcagg gaatgccaca
     1381 acgacaccac cagggacata aacgcacacc gaaacagttg gcgctcatta tcaaacgctg
     1441 tttgccgatg gtgctcactg gcagcggcat gctttgcact accgctaacg ccgaagagta
     1501 ttatttcgac cccattatgc tggaaaccac aaaaagtggt atgcaaacaa ccgatctgtc
     1561 acgtttttca aaaaaatacg cacaactacc aggaacttat caggttgata tctggctgaa
     1621 taaaaagaag gtttcacaga aaaaaattac atttaccgcc aatgcagagc aacttctgca
     1681 gccacagttt acggtagaac aactacgtga gctgggtatt aaggtggatg aaatcccggc
     1741 gctggctgaa aaagatgacg atagcgtgat caactcgctt gaacaaatca ttcccggtac
     1801 agctgctgaa tttgatttca atcatcagcg acttaatttg agcattcccc aaattgcact
     1861 gtaccgtgat gcaagaggtt acgtctcccc ttctcgttgg gacgatggta taccaacgct
     1921 gtttaccaac tactcgttta caggttctga taaccgttac cgccagggca atcgtagcca
     1981 acgacagtac ctaaatatgc aaaatggtgc caattttggc ccctggcgat tacgtaacta
     2041 ttctacgtgg acacgcaacg atcaggcgtc aagctggaac actatcagta gttatttaca
     2101 acgtgatatc aaggcgttga agtctcagtt gcttctggga gaaagcgcca ccagcggcag
     2161 tattttttcc agctacaact ttactggcgt gcaactcgct tccgacgata atatgttgcc
     2221 aaacagccag cgcggatttg ccccaacggt acgcggtatc gcaaacagta gtgcaatcgt
     2281 gactatcagg caaaatggtt atgtgatcta tcaaagcaac gtgccagcgg gtgcctttga
     2341 aattaacgat ctctacccct cttccaacag cggcgattta gaagtcacga ttgaagaaag
     2401 tgacggtacg caacgtcgct ttatccagcc ttattcttca ttacccatga tgcagcgacc
     2461 tgggcatcta aaatatagcg cgaccgctgg acgctatcgc gctgatgcaa acagtgatag
     2521 caaggaaccc gaatttgctg aagccacggc aatatatggt ttgaataata cttttacgct
     2581 gtatggcggc ctgctcggtt ctgaagatta ttatgcgctg gggatcggta tcggcggcac
     2641 acttggcgca ctgggcgcgt tgtcgatgga tatcaacaga gctgacaccc aattcgataa
     2701 ccagcactct tttcatggct atcaatggcg tacgcagtac atcaaagata tcccggaaac
     2761 caacaccaat atcgctgtca gctactatcg ctataccaac gatggctatt ttagttttga
     2821 tgaagccaat acccgcaatt gggactataa cagtcgccaa aaaagtgaaa ttcaattcaa
     2881 catcagccag acaatatttg atggggtaag tctgtatgcc tccggttcac agcaagacta
     2941 ttggggcaat aacgagaaaa acaggaatat ctctgttggg gtttccggcc agcaatgggg
     3001 aattggttac agcctgaatt atcaatacag ccgctacact gatcaaaata atgaccgcgc
     3061 actctctttg aatctcagta ttccgttaga acgctggtta ccgcgtagcc gggtttccta
     3121 tcagatgacc agccagaaag atcgcccaac ccaacatgaa atgcgtcttg atggctcact
     3181 gctggatgat ggtcgcctga gctatagtct ggaacaaagt ctggatgacg ataacaacca
     3241 taacagtagc gtgaacgcca gttaccgttc accttatgga accttcagtg ccggatacag
     3301 ttacggtaat gacagtagcc aatacaatta cggcgttacc ggcggcgtgg ttatccatcc
     3361 tcatggtgtg acactctcgc aatatctggg caacgctttt gcgcttattg atgctaacgg
     3421 ggcatctggc gtgaggatac aaaactatcc ggggattgct actgatccct ttggctatgc
     3481 agtggttcct tatctcacaa cttatcagga aaaccgtctc tcggtagata ctacgcagct
     3541 gcccgataac gtcgatcttg aacaaacaac acagtttgtg gtgcccaaca gaggtgcaat
     3601 ggtagcggcg cgtttcaacg ccaatatcgg ttatcgcgta cttgttacag tcagcgatcg
     3661 caacggtaaa ccgttgccct ttggcgctct tgccagcaac gatgatacgg ggcaacaaag
     3721 tatcgtcgat gagggcggca tactatatct ctctgggata tcgagtaaat cacaaagctg
     3781 gactgtacgc tggggaaatc aggcagatca acaatgtcag tttgctttta gtacaccgga
     3841 ttcagaacca acaacctctg tattacaagg cacagcgcag tgccattaag gataaaaaaa
     3901 tgaaaagagc gcctcttata acaggacttt tgttgatatc cacatcctgc gcttatgcct
     3961 cctcagaagg gtgtggagct gacagcacta gcggtgcgac aaattacagc agtgtggttg
     4021 atgatgttac ggtgaaccag acagataacg tgacaggacg ggagtttacc tctgcaacgc
     4081 taagtagcac taactggcaa tacgcctgtt cctgctctgc gggtaaggca gttaaacttg
     4141 tctatatggt cagccccgta cttaccacca ctggacatca gacaggatat tacaaactca
     4201 atgacagcct ggatattaaa accatgttat taaggttaat tttggattca tcaatggtca
     4261 gaagtttacc acccgcaatg ccatgccaga cggttttact ccagtagact ttgatatcac
     4321 ttatgactgt ggtgatactt caaagattaa aaactcgttg caaatgcgca tcgacggtac
     4381 aactggggta gtagaccagt acaacctggt cgccagacga agaagttcag acaatgcgcc
     4441 cgatgtcggt attcgtattg aaaatctcgg cggcggagtt gcaaatattc cttttcagaa
     4501 cggtatcctt cccgttgatc cttccgggca tggcaccgtc aatatgcgcg cctggccagt
     4561 taatctggtc ggtggtgagc tggaaacagg aaaatttcag ggcacagcca ccattaccgt
     4621 catcgtgcgg taaaagaaga cgttgcgttt tgattaacgc aacgcttcgg cactcttacc
     4681 cctgctgctc cagcgcatac ttatacagcg catttttctt cacgccgtga atttctgcgg
     4741 ccagcgccgc cgcttttttc agcggcagtt ctgcctgtag cagcgccagc gtgcgcaggg
     4801 catcggcggg taagtcttct tcctgtgctt tatgaccttc gacaatcagc accatttcgc
     4861 ctttgcgacg gttttcatct tcctttaccc acgccagcag ctcgccaacg ggcgcgccgt
     4921 gaatggtttc ccaggttttg gtcagctcac gcgccagaac cacgtagcgg gattcgccta
     4981 ataccgcaac gatatcttcc aggctatcta acagacggtg ggtagattca taaaaaatca
     5041 gcgtgcgcgg ctccgcttca atggctttta gcgcatcacg gcggcctttt gatttggcag
     5101 gtaaaaagcc ttcgtaacag aaacggtcag agggtaaacc cgcggcgctt aacgcagtga
     5161 tagcagcaca cggtccgggt agcggcacca cacgaatccc cgcttcacgg caggtacgca
     5221 ccagatggta gccaggatcg ttaattagcg gcgttccggc atcggaaacc agcgcaatgt
     5281 tttgcccctc ttgcagcttc gccagcagcg tttcggcttt ttgttgttcg ttatggtcgt
     5341 gcagcgcaaa caaccgggca ttaatcccaa aatgttgcag caataaaccg gtgtgacgag
     5401 tatcctcggc ggcaatcaga tcaacggcct gtaatacctc taacgcacgc tgggtgatat
     5461 ccgccagatt gccgattggc gtcggtacaa tgtaaagctg gccctgagaa ttatccgccg
     5521 attggtgttg tttcattgtg tcgtccgtat tgccgattta atattgagca ttgcgtaaaa
     5581 aaaatatcac tggatacatt atggtaccct caacattttc tcgtttgaaa gccgcgcgtt
     5641 gtctgcctgt tgttctggca gccctgattt tcgtcggttg tggcacccat actcccgatc
     5701 agtccactgc ttatatgcag ggcacggcgc aggctgattc tgccttttat cttcagcaga
     5761 tgcagcaaag ctctgatgat accaggatca actggcaatt actcgccatt cgtgcactgg
     5821 tgaaagaagg taaaaccggg caggcggttg agttgtttaa ccaactaccg caagaactga
     5881 acgattctca gcgtcgcgag aaaacactgc tggcggtaga gattaaactg gcgcagaaag
     5941 attttgctgg cgcgcaaaac ttgctggcga aaattacacc cgccgattta gaacaaaacc
     6001 agcaagcccg ttactggcag gcaaaaatcg atgccagcca ggggcgtcct tccattgatt
     6061 tactgcgcgc gttaattgct caggaaccgc tgcttggcgc gaaagaaaaa cagcagaata
     6121 ttgatgccac ctggcaggcg ctctcctcca tgactcagga acaggcgaat acgctggtga
     6181 tcaacgccga cgaaaatatt ctgcaaggct ggctggatct gcagcgcgtc tggtttgata
     6241 accgtaacga tcccgacatg atgaaagccg ggatcgccga ctggcagaaa cgttatccga
     6301 acaatccggg cgcgaaaatg ctgccaacgc agttggttaa cgtaaaagcg tttaaaccag
     6361 cctcgaccaa caaaatcgcc ctgctgttgc cactgaatgg ccaggcagcg gtatttggtc
     6421 gcactattca gcaaggcttt gaagcggcga aaaatatcgg cactcagcca gtggcggctc
     6481 aggtagctgc cgcacctgcc gcagacgtag cagaacaacc tcagccgcaa actgcggatg
     6541 gcgttgccag cccggcacaa gcctcggtta gcgatctgac cggtgatcag cctgcggccc
     6601 agccggtgcc tgtaagcgcc ccggcgacaa gcaccgcagc ggtaagcgca cccgcaaatc
     6661 catccgcaga gctgaaaatc tacgatacct catcacaacc acttagccag atcttaagcc
     6721 aggttcagca ggatggcgcg agtattgtgg ttggtccgtt gctgaaaaat aacgttgaag
     6781 agttgctgaa gagcaacact ccgctgaacg tactagcact gaaccagccg gagaatatcg
     6841 aaaatcgcgt caatatttgt tacttcgcgc tttcaccgga agacgaagcg cgcgatgcag
     6901 cgcgtcatat tcgtgaccag ggtaaacaag cgccgctggt gctgatccca cgcagttcat
     6961 tgggcgatcg cgtagccaat gcgtttgcgc aagagtggca gaaattgggc ggcggcaccg
     7021 ttctgcaaca aaaatttggt tccaccagcg aattacgcgc gggtgttaac ggcggttctg
     7081 gtattgcttt aacgggtacc ccgattactc ccagagcgac aaccgactcc ggcatgacga
     7141 ccaacaatcc aacgctgcaa accacgccaa ccgatgacca gttcaccaat aatggcggtc
     7201 gtgtcgatgc ggtgtacatt gtggcaacgc cgggtgaaat cgcttttatt aaaccgatga
     7261 tcgccatgcg taacggtagc cagagcggtg caacgctgta cgccagctcc cgcagtgcgc
     7321 aagggaccgc tggcccggat ttccgtctgg agatggaagg cttgcagtac agcgaaatcc
     7381 cgatgctggc gggcggtaat ctgccgttaa tgcagcaggc actcagcgcg gtgaataacg
     7441 attattcact ggctcgcatg tatgcgatgg gcgtcgatgc ctggtcgctg gcaaatcatt
     7501 tctcacaaat gcgccaggtt cagggttttg aaatcaacgg taataccgga agcctgacgg
     7561 ctaacccgga ttgcgtgatt aacaggaagt tatcatggct acagtaccaa caaggtcagg
     7621 tagtccccgc cagttaacca ccaaacagac cggcgatgcg tgggaagtac aagcgcgtcg
     7681 ctggctggaa ggcaaaggac tgcgatttgt cgccgctaac gtgaacgagc gtggcggcga
     7741 gatcgatctg ataatgcgtg aaggccggac caccgttttt gtcgaggtgc gttaccgccg
     7801 ttctgcgctt tatggcggcg cggcagccag tgtgacccgc agcaaacaac acaaattatt
     7861 acagactgcc cgcttgtggc tcgcgcgtca taatgggagt tttgatactg tggattgccg
     7921 gttcgatgtg gtagccttca ccgggaatga ggttgagtgg attaaggata cctttaatga
     7981 ccactcataa ttaaggttta aggattagcg tgcaagaaag aattaaagct tgcttcactg
     8041 aaagcattca aactcaaatt gcggcggcag aggcgcttcc ggatgccatc tcccgtgcgg
     8101 ccatgacgct ggttcagtct ctgctcaatg gcaacaaaat cctctgttgt ggtaatggaa
     8161 cttccgctgc caatgcacag cattttgctg ccagcatgat caaccgtttc gaaacggagc
     8221 ggcccagctt acctgccatt gcactaaata ccgataatgt tgtcttaacg gcgattgcca
     8281 acgatcgctt acatgatgaa gtgtatgcaa aacaggtgcg ggcgctgggg catgcgggag
     8341 atgtattgtt agccatttcc acccgtggca acagccgcga tatcgtaaaa gcagtagaag
     8401 ctgccgttac gcgtgatatg accattgtgg cattgaccgg ttatgacggc ggtgaacttg
     8461 caggtttgtt agggccacag gatgtggaga tccgcatccc ttcgcatcgc agtgctcgca
     8521 ttcaggaaat gcatatgctg acggtaaatt gcctgtgcga tctgatcgat aacacgcttt
     8581 tccctcacca ggatgattaa ggagaataca tgaaggcatt atcgccaatc gcagtcctta
     8641 tttccgcgct gctgttgcaa ggttgtgttg ccgctgccgt agtgggtact gctgctgtgg
     8701 gtaccaaagc cgcaactgac ccacgcagtg tcggcaccca ggtggacgat ggtaccctgg
     8761 aagtgcgcgt gaacagcgca ttgtcgaaag acgaacagat taagaaagaa gcgcgcatta
     8821 atgtaacggc ctatcagggc aaagtgctgc tggttgggca gtcaccaaac gctgaacttt
     8881 cggctcgcgc caaacagatt gctatgggcg tagacggtgc caacgaagtg tataacgaga
     8941 ttcgtcaggg ccagccgatt ggtctgggcg aagcgtctaa cgatacgtgg atcaccacca
     9001 aagtgcgttc gcagctctta accagcgacc tggtgaaatc gtccaacgtg aaagtgacca
     9061 ccgaaaacgg tgaagtgttc ctgatggggc tggtgactga acgtgaagcg aaagcggcgg
     9121 cagatattgc cagccgggtg agcggcgtga agcgggtaac tacggcgttt acgtttatta
     9181 aatagcagcc ccttgtaatg cctgatgcga cgcttgccgc gtcttatcag gcctacaacg
     9241 atgcaaaccg taggtcggat aaggcgttta cgccgcatcc gacatgtttt ccctcaaatc
     9301 agaataacag cgccagcccg ccgacaatca cgcctgacac tgccaccatc gcccccgtca
     9361 gccataaggc tttcgccggg aacgctttgc gcagcataat cagtgacggc aaactcaccg
     9421 ccgggagcgt catcaacaat gccagcgccg gagcagttcc catacctgcc agcatcatcg
     9481 tttgtacaat cggaatttct gctgccgtgg gaatgacaaa caagcaccct gctaccgcca
     9541 tcgccaccac ccacatcagg ctgttatcga cagcaccatc ggcatggggg aataaccaga
     9601 cgcgagcggc acccaacacc agtactgcaa ggatgtaaac cgggatcgta ctccagaaaa
     9661 gcgtccatag cgccctgccc cagcgactaa aaaatccgcc ctgtgcttcc ggtatgtcaa
     9721 tttcgaccgg tgcttgcgtt tgcggtgttt cacgcaccca tttttgcacc agcgtcgcaa
     9781 tcagcaacac catcaccagc ccggccacca gacgaatcgc cgcaaaaccc cagccgagga
     9841 caaaccccat aaacaccagc gtcgccgggt ttaacaccgg attgcccatc cagaatgcca
     9901 gcgcaccgcc catcgacacc tgttggcgac gcattcccgc cgcgaccgga gccgcacagc
     9961 aggtacacat catgcctggc agcgaaaaca gcgttcccag cagcgtgccg cgaaagcgcg
    10021 attgcccaag cgtacgcaac aaccagtcac gcgggatcag cacctgaatc aacgaaccga
    10081 ggatcacccc cagcaccgcc gctttccata ccgcgaggaa atagatcatc gcgtaatcca
    10141 acgccgcctg ccatggatta gcatccgcct gcgcaaggat agatttaccg atactgtggg
    10201 tttcggcagc agtaaacgct ttgccgtagt aaggttgcca tttcacatac cagagaccgg
    10261 caatgacaac gagaaagaaa agcgcgggtt tccaccactg aatgggcgtt gccgcctgag
    10321 atgaagactg accagtcata gcattcccca ggaataagtt atgataattg agcgcgtgaa
    10381 tattacgctc actatcaatt cttgggaata attattcagc tcttttgcgt aattctgaag
    10441 agcttaaaat cgtcacacct tcatgctcgg gccgcatcgc ctctgccagc atgacccgcg
    10501 ccacgtctct cgcatcaatg gatttccagt taccgggtaa caaacggaac agcggcgcaa
    10561 aaagcgtttc gttcatccgc tgtttgctac gatcgcccag taacatcgac gggcgagcaa
    10621 tggtcaattt cggccagttc tgggcgatta atgcttcttc catctcccct ttgacgcggt
    10681 tatagaaaaa cggcgagtgg gcattggcac ccatagcact gaccaccagc atatgctgcg
    10741 cgcccagtcg ccgcccggtt aatgcggtat ccactaccag cgtgtaatcg gcatgaataa
    10801 acgcctcttt gctccccgct tctcgccgcg tggtgccgag acaacaaaac acaatgtcga
    10861 tgggatcggt gacctgcgcc agcgcgtcag tcagttgcgg atcatgggga ttaaataccc
    10921 cgggcatatc gcccaacgga cgtcgcgtcg gcgcggctat ggcgttaact ttcggttcgt
    10981 taatcaacat ccgcagcagg tgaccgccca ccagccccgt tgcgcctgta atcagtacct
    11041 gactcatctt cgctccttta cagaattgtc tgccttgcgc tccacggctc atgcaccagg
    11101 cttaatagac catgaggtaa ttatccccga ttgtgggaaa ttcgcctcat ccaatgcaac
    11161 aacgcggagg aagcatgagt aagaaaattg ccgttttaat cactgatgaa tttgaggatt
    11221 cagaatttac ttcacccgca gacgagttcc gtaaagccgg acacgaagtg attaccattg
    11281 aaaaacaagc gggtaaaacg gtgaaaggca aaaaaggaga agccagcgtg accatcgata
    11341 aatccatcga tgaagtgacg ccagcggagt ttgatgccct gttgctaccg ggcggtcatt
    11401 caccggatta tctgcgtggc gacaaccgtt ttgtcacctt tacccgtgat tttgtgaata
    11461 gcggcaaacc tgttttcgcc atctgtcacg gtccgcagct gctgatcagc gccgatgtga
    11521 ttcgggggcg caaactgacc gcggttaaac cgatcattat tgatgttaaa aacgcgggcg
    11581 cggaatttta cgatcaggaa gtcgtggtcg ataaagatca gctggttacc agccggacac
    11641 cggacgatct gccagcgttt aaccgcgagg cgttacgcct gctcggtgcc tgagccgcgt
    11701 agccaaatca tttttttacc aaagcccagc gtgttgtcgg tgaatttgat ttcatcgggc
    11761 cggatttccc acaccggggc cgacagcatt ctggcaaccg gaaagcgacg attgtacgcc
    11821 ttgcgcgcga ggtcgctttc ctcaccttcc agcctgcgga tctcaccttt aaactgcacg
    11881 ccgcgaatta acgctaccgt tttcggctga ccgtttaccg ttccggcaac cgcagcctgc
    11941 ggcccacgca tctgcgcgtg gcgcgttttt tcttccgtca aaatgtagaa ggcgactttc
    12001 tgcacatcaa acagataaaa ggcattggcg caccagagtt ccccttcctg ctgcacacac
    12061 caggtgacaa catgttgttt tgccagccaa cggctgatgg cggtgagtgt ttccattgct
    12121 gttctctttt atactgtggg cctgacctta acatgcgacg cagacgatga caccctggtt
    12181 tctttacttg atccgtaccg ccgacaataa gctttatacc gggatcacca cggatgtcga
    12241 acgccgctat cagcagcacc aaagcggcaa aggggcgaaa gcactgcgcg ggaaaggaga
    12301 actgacgctg gcgttttctg cgccagtggg cgatcgttcg ctggcgttac gggcggaata
    12361 tcgcgttaag caactgacca aacggcaaaa agagcgtctg gtagcggaag gcgcagggtt
    12421 tgcaaagctg ttaagcagtc tgcaaacccc ggagattaaa agcgattgaa atgctcgtga
    12481 tactcaacca gaccagttac gccattcaac gcgtcatccg ctaaacgatg tacctggaag
    12541 gcgctttcag tgcccggcca gcggcaacgc agatcgtgat gcgccgccag ttcaaagccg
    12601 aaacggctgt atagcgccgg atcgcccagc gtcaccactg cggcatagcc gaactcatta
    12661 agcgaatcga gtccttcata gaccagttgg cgtgccagcc cctgtccacg gtatttttca
    12721 tctaccgcca gcggtgccat gccgacccat tgcaggtctt cgccctgcac atcaaccgga
    12781 ctaaatgcca catagccaat gacctgacct tcgtcatctg tcgccaccag ccccagcgtc
    12841 agaaagccat cttcacgcag atcgtgaacc agcttcgctt ccgcatcact ttcgaatgaa
    12901 cgacgcagca gggcatcaat acccggcgca tcaatgggaa tttctactcg aattagcatg
    12961 gttcacctac cgatgtctgt ttggtttcag gcgcggtttt cattcccgcc tcaacaaaat
    13021 ccgccagttg cagcagcatc atgcgtaagg ctttcggcat ttgctccagt tcaatggcgt
    13081 ccatcaggtt tttcacatac agccccagct ccgtatcgcc ttcaatcacc agccgacgct
    13141 ggaagaagag cgtatccgga tcttgtttac gcgccgcaat catcagcaga tcgctggcgt
    13201 cggcactaaa actcacatca gcttgcgcgt tctggctgac caccagtttg ccattcacca
    13261 ccgaggtaaa ccattgcagg tcaatatcac gcacatgaat acttaaccag cggccttcaa
    13321 gaaactccag ctcaccatca tccagcgcct ggcggaattg ccagcttaag acctgctcaa
    13381 gaacctggcg ttttagcgca aatggcgtca gttttaccgg tacactcaac agagatggcc
    13441 ccaaatgcac aatacgggaa cgcagtttat ccaacacgag ttttactccc tgtttcaaca
    13501 atcatcctat tttgccatat cagaaaaata acatagcggt ataaatcaac aattccatat
    13561 gaaattgctg ctaccaccaa tacaacttta actgccttaa atcaaaaatt gtcgcagcaa
    13621 ggttaactaa aatcccagtt cgttaacatt tttgcgtttt gatagcgcaa ccttcaggaa
    13681 aaattatgga gctgctctgc cctgccggaa atctcccggc gcttaaggcg gccatcgaaa
    13741 acggcgcaga tgctgtttat atcgggctaa aagatgatac caatgcccgt cacttcgccg
    13801 gccttaactt taccgagaaa aaattgcagg aagcggtgag ttttgtccat caacatcgcc
    13861 gcaaacttca catcgcgatt aacacttttg cgcatccgga cggttacgcc cgttggcagc
    13921 gtgccgtgga tatggcggcg cagctgggtg ccgacgcgct gatcctcgcc gacctcgcca
    13981 tgctagagta cgccgccgag cgttacccgc atattgagcg tcatgtgtca gtgcaggctt
    14041 cggcgaccaa tgaagaggcg attaactttt atcatcgcca ttttgacgtt gctcgcgtgg
    14101 tgctgccgcg cgtgttgtcg attcatcagg tgaaacaact ggcacgggtc acacctgtcc
    14161 cgctggaagt ctttgctttc ggcagcctgt gcattatgtc ggaaggtcgt tgctatctgt
    14221 cgtcgtatct gacgggtgag tcgcccaaca ccgtgggcgc gtgttctccg gcccgtttcg
    14281 tgcgctggca gcaaacgccg caggggctgg aatcccgcct gaacgaagtg ctgatcgacc
    14341 gttatcagga cggcgaaaac gcaggttatc cgacgctatg taaagggcgt tatctggtgg
    14401 acggcgagcg ctatcacgcg ctggaagaac caaccagtct caataccctg gaactgctgc
    14461 cggagttaat ggcggcgaat attgcttcgg tgaaaattga aggccgccaa cgtagcccgg
    14521 cgtatgtcag ccaggtggcg aaagtctggc gtcaggctat cgaccgttgt aaggccgatc
    14581 cgcaaaactt cgtaccgcaa agcgcgtgga tggagacgct cgggtcgatg tccgaaggca
    14641 cgcagaccac ccttggcgcg tatcaccgta aatggcagtg agaagagcaa tgaaatattc
    14701 cttagggcca gtgctgtggt actggccaaa agagacgctg gaagattttt atcagcaggc
    14761 cgccaccagc agcgccgacg tgatttatct tggcgaagcg gtttgcagca agcgccgggc
    14821 aaccaaagtt ggcgactggc tggagatggc aaaatcgctc gccggaagtg gtaagcagat
    14881 tgtgctctcc acgctggcgc tggtgcaggc atcatctgaa ctgggcgaac tgaaacgcta
    14941 tgttgagaac ggtgagtttc tgattgaagc cagcgatctc ggcgtggtga atatgtgcgc
    15001 tgaacgcaaa ctgccgttcg tcgccggaca cgcgctgaac tgctataacg cggtgacgct
    15061 gaaaatattg ctcaaacagg gcatgatgcg ctggtgtatg ccggtggagc tttcccgcga
    15121 ctggctggtg aatctgctta atcagtgcga tgagctgggc attcgcaacc agtttgaagt
    15181 tgaagtcctg agctacggtc atctaccgct ggcctactcc gctcgctgct ttaccgcgcg
    15241 ttcggaagac cgcccgaaag atgagtgtga aacctgctgc attaagtatc cgaacgggcg
    15301 caacgtgctg tcgcaggaaa accaacaagt gtttgtactc aatggcattc agaccatgag
    15361 cggctacgtt tacaacctcg gtaacgagct ggcatccatg cagggcctgg ttgatgtggt
    15421 ccgcctgtca ccgcagggta ctgacacttt cgcgatgctc gacgccttcc gcgctaatga
    15481 aaatggcgct gcaccactgc cgttgacggc gaacagcgac tgtaacggct actggcggcg
    15541 gctggcagga ctggagctgc aagcctaagt aaatagctca ctttgttaac aactttaact
    15601 actctttaat gcagtattaa agattaatcg gtaacaaagt gagctgttat gactgataaa
    15661 accattgcgt tttcgctact cgatctggcc cccattcccg aaggttcttc agcgcgagat
    15721 gcattctccc actctctcga tctcgcccgt ctggctgaaa agcgcggcta tcatcgctac
    15781 tggctggcag aacaccacaa tatgactggc attgccagtg ctgccacgtc ggtattgatt
    15841 ggctatctgg cggcgaatac caccacgctg catctggggt ctggcggcgt gatgttgcct
    15901 aaccactcac cgttggtcat tgcagaacag ttcggcacgc tcaatacact ctatccgggg
    15961 cgaatcgatt tggggctggg tcgtgctccg ggtagtgacc agcgaaccat gatggcgcta
    16021 cgtcgtcata tgagcgggga tattgataat ttcccccgcg atgtcgcgga gctggtggcc
    16081 tggtttgacg cccgcgatcc caatccgcat gtgcgcccgg taccaggcta cggcgagcaa
    16141 atccccgtgt ggttgttagg ctccagcctt tacagcgcgc aactggcggc gcagcttggt
    16201 ctgccgtttg cgtttgcctc acacttcgcg ccggatatgt tgttccaggc gctgcatctt
    16261 tatcgcagca acttcaaacc gtcggcacga ctggaaaaac catacgcgat ggtgtgcatc
    16321 aatattatcg ctgccgacag caaccgcgac gctgaatttc tgtttacctc aatgcagcaa
    16381 gcctttgtga agctgcgccg tggcgaaacc gggcaactgc cgccgccgat tcaaaatatg
    16441 gatcagttct ggtcaccgtc tgagcagtat ggcgtgcaac aggcgctgag tatgtcgctg
    16501 gtgggcgata aagcgaaagt gcgtcatggt ttgcagtcga tcctgcgcga aaccgacgcc
    16561 gatgagatta tggttaacgg gcagattttc gaccaccagg cgcgcctgca ttcgtttgag
    16621 ctggcgatgg atgttaagga agagttgttg ggatagtgtg tcttaacgcg ggaagcctta
    16681 tccgagctgg caacgctgtc ccacgtaggc ctgataagcg aagcgcatca ggcattgtgt
    16741 aggcagcaga aatgtcggat aaggcccgct gattactgat acaccggcag caaattaaag
    16801 ctcgataaga tatggaccag tgcgttgcca acgccaaata ccagaatcag cgtaatcatc
    16861 ggcttgccgc cccagacgcg gaatttcggg ctgccaaagc gtttacgcga tgcgcgggcc
    16921 agcagtgccg gaacaattgc cgcccagatg gtagctgcta aaccagcata accaatggcg
    16981 tacaggaatc cgttcgggaa cagcagacca cccacaacag gaggggcaaa ggtcagcaat
    17041 gccgttttca agcggcccac agccgagtcg tcgaaaccaa acagatctgc cagatagtca
    17101 aacaaaccca gcgttacgcc gaggaacgaa ctcgctaccg caaagtttga gaacacgacc
    17161 agcagcagat ccagactacg gctgttcagt acgccgctta acgcctgtac cagcacatca
    17221 atattaccgc ccttctctgc aatatcgata aactccggac gcgggatgtt acccatcgtc
    17281 gccagcaacc agatggtata cagcgccagc gccatcagcg taccgtacac cagacatttc
    17341 acgatggttt tcggatcttt gccgtaatac ttcatcaggc ttggcacgtt accgtgataa
    17401 ccaaacgatg ccagacagaa cggcaaggtc atcaacagat acggtgcata agacgcattg
    17461 ctttcagcga cgttgaacaa tgtcgcaggc tgcacatgcc ccagcaggct accgaaggtg
    17521 aggaagaagg taatgacttt cgcccccaat acaatcgctg tcatgcgact gacggcttta
    17581 gtgctcaacc acaccacaaa cgctaccagc aatgcaaaac caaaacccgc cgcccgtgcc
    17641 gggacgttta gtgacatctc tgcgaaggtg tgatgcagaa tcgaaccact ggcagaaata
    17701 taggcatagg tcaggatata gagcacaaag gcaatggaaa tgccgttgac cacgttccag
    17761 cctttgccca gcaaatcttt ggtgatggtg tcaaaactcg aaccgattct gtaattcagg
    17821 ttagcttcca gaatcatcaa gccggaatgc agcatacaga accaggtaaa gatcagcgcc
    17881 gccattgacc agaaaaacca cgccccggac atgaccactg gcagagaaaa catccctgcg
    17941 ccaataatgg tgccgccgat aatcaccacg ccgccaagca gcgacggtga cgtttgggtg
    18001 gtggttagtg ttgccatgag ggcttctctc cagtgaaaaa tagtgcgact gcgttgttat
    18061 gtattgcact gtaccagtac gcgagtacaa aagacagaaa aaaagccccg atggttcaaa
    18121 tcggggctgt atatattatt ttacagactg tgttcgctgt tcagcgatga ttacgcatca
    18181 ccaccgaaac gacgacgacc ggtagagtca tcacgacgcg gagcgcggcc ttcacgacgt
    18241 tcgccgctaa aacgacgacc atcaccacga cgctcgccac tgaaaccacg accaccgcca
    18301 cgacgctctc cgccagtatg cggctgtgca tcgcccagta attgcatatt catcggcttg
    18361 ttcagaatgc gagtacgtgt aaagtgttgc agcacttcac ccggcatacc tttcggcagt
    18421 tcgatggtgg agtgagaagc aaacagctta atgttaccga tgtaacggct gctgatgtcg
    18481 ccttcgttag caatcgcacc aacgatatga cgaacttcaa caccatcatc gcggcccact
    18541 tcaatacggt acaactgcat gtcaccaaca tcacgacgtt cacgacgcgg acgatcttca
    18601 cggtcgccac gcggaccacg gtcgttacga tcgcgcggac cacggtcatc acggtcacgg
    18661 aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg ttcaccctgt
    18721 gccattttca gcagtgcggc ggccagagtt tcgagatcca gctcttcacc ttctgcagtc
    18781 ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc cagctgctgc
    18841 tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc gttcggcagt
    18901 tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag acgacgctcg
    18961 cggttctcaa cgaacagcag cgcgcggcca gcacgacccg cacgaccggt acgaccgata
    19021 cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag gctgatacgc
    19081 tcaacgtcca ggccacgggc tgcaacgtcg gtcgcaatca ggatgtccag acgaccatct
    19141 ttcaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt cagcgcggcg
    19201 ctgttgtagc cgttacgctc aagagcttca gccacttcca gagtcgcatt tttggtacga
    19261 acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag tgcttcgttt
    19321 ttacgcatac cccagacagt ccaatagctc tggctgatgt caggacgggt agtcacgctg
    19381 gactgaatgc gcacttcctg cggctctttc ataaagcggc gggtaatgcg acgaatcgct
    19441 tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg cgccataata
    19501 gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc cagaaccaga
    19561 ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag acggcccgga
    19621 gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc ataacgctgg
    19681 ccgccgtaca gggcaaccac atttacgccg cgcatgtgtt tagagaaatc cgtcattgct
    19741 tcagcaacct gtaccgccag ttcgcgggtc ggtgctagca ccagaatctg tggtgctttc
    19801 agctcaggat caagattttg cagcagaggt aaagagaatg ctgcagtttt tccgctcccc
    19861 gtctgggcca tacccagtac gtcgcggcca ttcagcagat gtggaataca ctccgcctga
    19921 attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg agccttcagg
    19981 cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct caaaattaat
    20041 ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg aaaagtgtga
    20101 accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa ggtgatggca
    20161 atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat tctgccaggt
    20221 catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc tcaacaaagt
    20281 tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc aaatccccca
    20341 gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca gcgagcgagg
    20401 tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta atgttgccca
    20461 ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg aagtgctgtt
    20521 tcaacacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa agccacagac
    20581 tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct tgcgctaact
    20641 tgtcacgacc gccgtaatat aatgcgatcc cgcgattcaa gtgcgcgtag ttgtaagttg
    20701 gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg cctgcctgcg
    20761 ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt gccagcgctt
    20821 gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac actccgcgct
    20881 catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg atttgttcca
    20941 tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg aggacttcac
    21001 ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca acgaaacacc
    21061 agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa cgtcctgttc
    21121 ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg gagggcaaat
    21181 ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca ggttgagact
    21241 gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact tccagaactt
    21301 tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg cgtttgtcag
    21361 cgatttgaga gatgtggacc agaccttctt taccgccgcc gatggcaaca aatgcgccga
    21421 agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcaatt tctgcagtga
    21481 tctcttcgat acgacgaata gcgtgtttcg ctttctcgcc atcggtcgct gcgatcttca
    21541 cagtaccgtc atcttcgatt tcgatggtgg tgccagtttc ttcggtcagg gcacggatca
    21601 cagagccgcc tttaccgata acgtctttga tcttgtccgg gttgatcttg atggtatgga
    21661 tacgcggagc gaactcagag atatcgccac gtggcgcgtt gatcgcctgt tccattacac
    21721 ccaggatgtg cagacgcgca cctttagcct ggttcagtgc aacctgcatg atctctttgg
    21781 tgataccttc aattttgata tccatctgca gtgcagagat accgtcgcgg gaacccgcaa
    21841 ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac agtacaacgt
    21901 agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct ttgattggca
    21961 cacctgcgtc catcagcgcc agagacgcac cgcacacgga agccatagaa gaagaaccgt
    22021 tggattcagt gatttcagac acaacacgta cggtgtacgg gaatttgtcc atatccggca
    22081 ttactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga cgcttcggag
    22141 aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg aacaggaagg
    22201 tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca gtacccagcg
    22261 ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg tgagtacgcg
    22321 gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga ccatcgatac
    22381 gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatcgcg tgcagaattt
    22441 cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg atggtttcag
    22501 atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg taagcatcgc
    22561 tcagacgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt accggctccg
    22621 gctgccagtc ccaacgcggt ttaccggctt ctttcaccag ttcattgatg ttctgaataa
    22681 caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct tcgctcagca
    22741 gttcagcttc agattcaacc atcagtacag cggcttcagt acccgcaaca accagatcca
    22801 gtttgctctc tttcagctcg tcctgagtcg ggttcagtac gtactggtca ttgatgtaac
    22861 ctacgcgggc agcaccaatc gggccattga acggaatacc agacagagac agtgctgcgg
    22921 aagcaccaat catcgcgacg atatccgggt taacctgcgg gttaacagaa accacggtgg
    22981 cgataacctg aacttcgttg acgaagcctt ccgggaacag cgggcgaatc gggcggtcaa
    23041 tcagacgcgc gatcagggtt tcgccttcgc ttggacgacc ttcacgacgg aagaagctac
    23101 ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt gggaagaagt
    23161 cctgacctgg tttggctttt ttctggccaa caacggtaac aaataccgcg gtgtcatcca
    23221 tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc agagtcacgg
    23281 tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata tcctttctct
    23341 ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc atcctcgcga
    23401 ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc gcgcgaacct
    23461 ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt gctgccgtca
    23521 gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat tagcgacgca
    23581 gacccaggcg ctcgatgagc cgggtgtaac gtgctacgtc tttacgtttc aggtagtcga
    23641 gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg tgatcttttt
    23701 tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt gctacctgaa
    23761 cttcggtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt ttagctgttg
    23821 cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaaag acgccgatct
    23881 ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg cgacgttaag
    23941 cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta cgccgggtat
    24001 tcaaccacca gacgacgagg cgcaacgcgg ccttcatcgt caatttcgcc cataccgata
    24061 aacttgccgt tctcaccttc cgtaacgcga accagtcctt ccagcggcgc gccagatgta
    24121 cgaaccgggt taccattttt gaagtaaaca gaagacgtta acggaagatt caccaccggg
    24181 tagtccgaag ctggactgtc cattggcatc agtaatggat caagcaactc cgcagccgga
    24241 atatcctgct gttcggcttg ttcgaccagt tcacgcaggt gctccagggt caccatccgt
    24301 tcaaccggat atttacttac cgccagacga cgcaggtaaa tcacatgcgc gccacagccg
    24361 agtttttcac ccaggtcatc aatgatggtg cggatataag tgccttttga gcagtgaatt
    24421 tccagctcca gctcattgcc ttcatggcga ataaacagca attcataaac ggtaattgga
    24481 cgcgcttcac gcggaacttc aatgccctga cgcgcatatt cgtacagttt tttgccctgg
    24541 tatttcagtg ctgaatacat cgatgggatc tgttcgatat cgccacggaa agtatccagt
    24601 gccgccgcca gttgttctgc actaaaggtt accggacgct cttcaacgat ttgtccgtcg
    24661 acgtcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg tttgtcggag
    24721 tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaatcggcaa catgccggtc
    24781 gccagcgggt ccagcgcacc ggtatgcccg gcacgattgg cgttatatat acgtttcact
    24841 ttttgcagcg catcgttgct ggacataccc tgcggtttat ccagcaacaa aacgccgtta
    24901 atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg tccgggttaa
    24961 cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacata cgcatccctt
    25021 caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg cgcatcgctt
    25081 tacccagcag gctgcggatg aaaccagaag cttcctgcaa cgctttgatg cccgctttaa
    25141 ccgcgtcttc atctttgtcg ttgaggaaag tcacatacac tttggcatac gccaggtcgc
    25201 gagacatttc gacaccggaa acggtggtca tcatgcccag acgaggatct ttaatttcac
    25261 gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc gggcgaccaa
    25321 attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc tttttaaaaa
    25381 ttaatttcag gtggaagggc tgttcacgtt ggcctgataa gacgcgccag cgtcacatca
    25441 ggcaatccat gccggatgcg gcgtaaacgc cttatcccgc atggaaccct aaaaacctta
    25501 agcgatggta cgttggatct cgatgatttc gaatacttcg atcacatcgc cagtgcggac
    25561 gtcgttgtag ttcttaacgc cgataccaca ttccataccg ttacggactt cgttaacgtc
    25621 atctttgaag cggcgcaggg actccagctc gccttcatag ataaccacgt tgtcacgcag
    25681 aacgcggatc gggttgtgac gtttaaccac accttcggta accatacagc ctgcgatggc
    25741 accaaatttc ggcgatttga acacgtcacg aacttccgcc agaccgataa tctgctgttt
    25801 cagttccgga gacagcatac cgctcatcgc cgctttcact tcgtcgatca ggttatagat
    25861 gacggagtag taacgcagat ccaggctttc cgcttcaatc actttacgtg cagaggcatc
    25921 agcacgtacg ttgaagccaa ccaggatggc gttggacgcc gcagccaagg tggcgtcggt
    25981 ttcggtgata ccacctacgc cagaaccgat gatcttcact ttaacttcgt cagtagacag
    26041 tttcagcaag gagtcggaga tcgcttcgac agaaccctgt acgtctgcct tcaggacgat
    26101 attcacttcg tgaacttcgc cttcggtcat gttggcgaac atgttctcga gtttagattt
    26161 ctgctgacgc gccagtttaa cttcgcggaa tttaccctga cgatagagtg caacttcacg
    26221 cgctttcttc tcgtcacgta caacggtaac ttcatcaccc gcagccggaa caccggacaa
    26281 gccgaggatt tccaccggaa tggacggacc cgcttccagc acttcctgac ccagttcgtt
    26341 acgcatcgca cgaacacgac cgtattcgaa gccacacaga acgatatcgc ccttgtgcag
    26401 agtaccttca cgtaccagaa cggtagcaac cggaccacga cctttatcga ggaaggattc
    26461 gataaccgca ccgctcgcca tacctttacg taccgctttc agctccagaa cttccgcctg
    26521 cagcaggata gcgtccagca gttcatcgat accggtaccc gctttcgcag atacgtgtac
    26581 gaactggctt tcaccgcccc actcttccgg caggatgccg tactgggaga gttcgttctt
    26641 aacgcgatcc ggatcggctt ctggtttatc gatcttgttc actgcaacca ccaccggtac
    26701 ctgcgccgct ttcgcgtgct ggattgcttc gatggtctgc ggcattacgc cgtcgtcggc
    26761 agcaacaacc aggactacga tgtcagttgc ctgcgcacca cgtgcacgca ttgaagtaaa
    26821 cgcggcgtgc cccggggtgt ccaggaaggt gatcatgccg ttttcagttt caacgtggta
    26881 tgcaccaatg tgctgggtaa tgccgcccgc ttcgccagag gccactttcg ttgaacgaat
    26941 gtagtccagc agagaggttt taccgtggtc aacgtgaccc atgatggtca caaccggagc
    27001 gcgcggttca gccgcagctc ccgtgtcacg gtcgctcatt accgcctctt ccagctcgtt
    27061 ttcacgacgc aggataactt tgtggcccat ctcttcagca accagctgtg cggtttcctg
    27121 atcgataacc tggttgatgg ttgccattgc gcccagtttc atcatcgctt tgatgacctg
    27181 agagccttta accgccatct tgttcgccag ttcgccaacg gtgatagttt cgccgatcac
    27241 aacgtcacgg ttaacggcct gagcaggctt ctggaagcct tgctgcagcg aagaaccttt
    27301 acgttttccg cccttaccgc cacgtactgc tgcgcgtgct tcttcacgat cagcttttga
    27361 ttcagcgtgt ttgttgcctt tcttcggacg cgctgctttc gcgttacgac cacggccacg
    27421 gccgccttcg acttcacgat cgctttcgtc ttctgcctgg cgagcatgtt gagaagtagt
    27481 gacgtgataa tcgctggaat cctcagtcgg ttccgcgtta tcagtccatt tgttttcttc
    27541 cgccatacga cgtgcttctt cagcaacgcg acgtgcttct tcttcgagtt tacgacgcgc
    27601 ttcttcttcg gctttacgct tgagctctgc agcttcctgc tcacggcggg ctttttcagc
    27661 ctgggcgttt ttagtcatat cgtcttgttg attgctcact ttgtcttttt ccgcagcttc
    27721 acgtttcgct tgttcagcag cttcacgctt agcttgttct gcggcctcac gttcagcttt
    27781 ttgttgcgcc tcgcgtttag ccgattcttc tgcctcacga cgggcttgct cttccgcttc
    27841 acgctgcgct tgctcttccg ctgcaaggcg ttcagcctct tgcggatcgc gtttcacaaa
    27901 ggtgcgtttc ttgcggactt cgatttgtac cgatttgctt tttccaccgg taccaggaat
    27961 gttaagggtg ctgcgtgttt tacgttgcag cgtcaatttg tccgggcctg aatttttctg
    28021 attcaggtgg tcaatcaaag tctgtttctc ttgtgcagac acagagtcgt cagcagactt
    28081 ccggatacct gcatcagcaa attgctgtac caggcgttcc acggaggtct gtcgctctgc
    28141 ggccagcgtt ttaatcgtta catctgtcat gctgttcctt cctgctacag tttattacgc
    28201 ttcgtcaccg aaccagcaaa tattacgggc agccataatc agtgctccgg ctttttcgtc
    28261 ggtcaaccct tcgatatcag ccagatcatc aatgccctgt tcggcgagat cttccagcgt
    28321 acaaacgcca cgggcggcca gtttgaatgc caaatcacga tctacccctt caaggttcag
    28381 cagatcgtca gccggtttgt tatcaccgag gctttcttcc tggacctgtg caatggtggc
    28441 cagtgcattt ttagcacgct cgcgcagtgc ttcaacggtc ggctcatcaa ggccttcgat
    28501 ttccaacagc tctttcatcg gcacataggc cagttcttcc agcgtcgaga agccttcctc
    28561 taccagaaca gtcgcgaagt cttcgtcgat gtcgagatat ttggtgaagg tgtcgatcgc
    28621 tgcgtgcgct tccgcctgat gcttagcctg caggtcgtca acggtcatca cgttgagttc
    28681 ccaaccgctc agctgcgaag ccagacgcac gttctgaccg ttacggccaa tcgcctgcgc
    28741 caggttaccg gcttcaacgg cgatatccat ggtgtgttta tcttcatcca ccacgataga
    28801 agcaacgtct gccggtgcca ttgcgttaat cacaaactgc gccgggttat catcccacag
    28861 gacgatatcg atacgctcgc cgcccagttc agtagaaacc gcctgaacac gcgcgccacg
    28921 catacctacg caagcaccta ccggatcgat acgtttatcg ttggttttca ccgcgatttt
    28981 cgcacgagaa cccgggtcgc gagccgctgc tttaatttca atcacttctt cgccgatttc
    29041 tggcacttca atacggaaca gttcgatcag catttccggc ttggaacgag tgacgaacag
    29101 ttgcgcgcca cgcgcttccg ggcgaacgga ataaagcacg ccacgaacgc ggtcgccagg
    29161 gcggaagttt tcacgcggca gcatatcttc gcgcaggatc acggcttcag cgttgttgcc
    29221 cagatccaga gagatgttgt cgcggtttac ttttttcacc acgccggtga tgatttcacc
    29281 ttcgtgttca cggaactgat caaccaccat cgcacgttcg gcttcacgca ctttctgcac
    29341 gataacctgt tttgccgtct gggtggtgat acggtcaaag gtaacagact caatctgatc
    29401 ttcaacgtaa tcgcccaggt tcaggctttc atcttcataa cgtgcggctt caagggtgat
    29461 ttccttggtc ggctgggtga cttcatcaac aactaaccag cgacggaagg tgtcaaaatc
    29521 accgcttttg cgatcgatct gtacgcggac gtcgatctct tgttcatatt ttttctttgt
    29581 tgctgtcgcc agcgcgcttt ccaatgcttc gaaaatcttc tcgcgaggta gcgccttttc
    29641 attggatacg gcttcaacta cagccaaaat ttctttgttc atcgcgggct tttcacctca
    29701 tccagactat taaaagtggg gaaccaagtt cgccttctgg atattactca gcgcgaacac
    29761 ttcatcttta ccttcgacgg taactgtgat catttcaccg tctaccgctt tgataacgcc
    29821 ctgccattta cgacggtttt gtaccgccat acggagaacc agagtcacct cttctccgac
    29881 aaaacgggcg tagtgttcag ccgtgaacag tgggcgatcg agacccggtg aggagacttc
    29941 caggttataa gcaacggtga tgggatcttc aacatccagc acagcactta cctggtggct
    30001 cacatcagca caatcatcaa cattgatgcc atcttcacta tcaatataga tgcgcagtgt
    30061 ggatgtgcga ccgcgaataa attcgatgcc aaccagttca aaacccaggg cctcaactgg
    30121 cgcagtaatc atctctgtta atttttgctc taatgtggac aagcccaccc ccaagacata
    30181 aaaaaagggc ctaaagccca gttattctgt agtcagataa caaaaaaccc cgataaatcg
    30241 gggctttata taactgaacc ctataaccgc aactgcggtc tggagcactt tccagaagga
    30301 ttttttcaaa tcccactacg aaggccgaag tcttcacagt atatttgaaa aaggactcta
    30361 agggaaagtg gttgcggggg ccggatttga accgacgacc ttcgggttat gagcccgacg
    30421 agctaccagg ctgctccacc ccgcgcctga aacgtggcaa attctactcg ttttgggtaa
    30481 aaaatgcaaa tactgctggg atttggtgta ccgagacggg acgtaaaatc tgcaggcatt
    30541 atagtgatcc acgccacatt ttgtcaacgt ttattgctaa tcacgtgaat gaatatccag
    30601 ttcactttca tttgttgaat acttttgcct tctcctgctc tcccttaagc gcattatttt
    30661 acaaaaaaca cactaaactc ttcctgtctc cgataaaaga tgattaaatg aaaactcatt
    30721 tattttgcat aaaaattcag tgagagcgga aatccaggct catcatcagt taattaagca
    30781 gggtgttatt ttatgacgac gattctcaag catctcccgg taggtcaacg tattggtatc
    30841 gctttttccg gcggtctgga caccagtgcc gcactgctgt ggatgcgaca aaagggagcg
    30901 gttccttatg catatactgc aaacctgggc cagccagacg aagaggatta tgatgcgatc
    30961 cctcgtcgtg ccatggaata cggcgcggag aacgcacgtc tgatcgactg ccgcaaacaa
    31021 ctggtggccg aaggtattgc cgctattcag tgtggcgcat ttcataacac caccggcggc
    31081 ctgacctatt tcaacacgac gccgctgggc cgcgccgtga ctggtaccat gctggttgct
    31141 gcgatgaaag aagatggcgt gaatatctgg ggtgacggta gcacctataa aggaaacgat
    31201 atcgaacgtt tctatcgtta tggtctgctg accaatgctg aactgcagat ttacaaaccg
    31261 tggcttgata ctgactttat tgatgaactg ggcggtcgtc atgaaatgtc tgaatttatg
    31321 attgcctgcg gtttcgacta caaaatgtct gtcgaaaaag cttactccac ggactccaac
    31381 atgcttggtg caacgcatga agcgaaggat ctggaatacc tcaactccag cgtcaaaatc
    31441 gtcaacccga ttatgggcgt gaaattctgg gatgagagcg tgaagatccc ggcagaagaa
    31501 gtcacagtac gctttgagca aggtcatccg gtggcgttga acggtaaaac ctttagcgac
    31561 gacgtagaaa tgatgctgga agctaaccgc atcggcggtc gtcacggcct gggcatgagc
    31621 gaccagattg aaaaccgtat catcgaagcg aaaagccgtg gtatttacga agctccgggg
    31681 atggcactgc tgcacattgc gtatgaacgc ctgttgaccg gtattcacaa cgaagacacc
    31741 attgagcagt atcacgcgca tggccgtcag ttgggccgtc tgctgtacca ggggcgttgg
    31801 tttgattccc aggcgctgat gctgcgtgac tctctgcaac gctgggttgc cagccagatc
    31861 actggtgaag ttaccctgga gctgcgccgt gggaacgatt attcaatcct gaataccgtc
    31921 tcagagaacc tgacctacaa gccagagcgt ctgacgatgg aaaaaggcga ctcggtgttc
    31981 tcgccagatg atcgtatcgg tcaactgacc atgcgcaacc tggatatcac cgatacccgc
    32041 gagaaacttt tcggttatgc caaaactggc ctgctttcct cctctgccgc ttcaggcgtg
    32101 ccgcagatgg agaatctgga aaacaaaggc cagtaattcg acatcaaccc tgcccttcag
    32161 gggtggggtt accttgctta tcctctgtaa actcctttct gagcatcaca taatcgaaaa
    32221 catcatggtt tgcatccaca accagtgagt ctcctttata gtgtacaatc acttcctcaa
    32281 gcgtttgcga ctgttccggc tttgttaccc ccatccacgc attaataagg taattattgt
    32341 aagctgtcga aaagatgttg ttttccgttg tgcgatccac accatcccca agaacggggc
    32401 tataccagat aaacatcgga acatgatatg cctgctggct ggcctccctg cctccatgaa
    32461 aatagacgtt cttcttcgta gggtcacgtt ccaggccgtg gtcggcaaaa tacatgaccg
    32521 aggcgcggcg atcttttaat aattcaaaaa cctgacctag caaactatct gtgtaatgaa
    32581 tggagttgtc atagcaggca tcctgatcgt cctgcggttg aaacacggcg ctggattgcg
    32641 gataggcgct acaagccggt tcatggcttc catttaaatg aagaacaatc agtttctttt
    32701 gctgcgtatt ttgctgtaat gcttgcgata aatgcggcaa caacaattca tcaaatcctc
    32761 tgacataaac tgtttccatg gcacgcatgg cgatactggt aactgctgta ccattctgcc
    32821 gaaaagcgga ttgcgagctt agccagaaag tctgaaatcc tgcttgatta gccatattaa
    32881 taatgttgtc ggggtaatta tgaatgtcat ggctcaaaac agaatcagca gttaaagaaa
    32941 ggggaaccga cagcgcagtg taaggtgcac cgcttattgc ttgattaaac agtttgatct
    33001 gttttctttg tgcttcgact tgcggtgtcg tagagcgtgt atatccatac aaagacatat
    33061 tgtcgacacg tacagactcc cccacaatca acacgtaggt atcaatacct gtatccctga
    33121 ctgataattg aaaatacggc actgtgtttg caattgagag taatctttga tgctctttcg
    33181 ctgctaaagc aaaataattg agattgaaaa acggcgtata ggtagcaaat cgcgacgcta
    33241 gtatatatgg actgaacgca tttttatttt tggcatcttt ataagcaaat tgacaagcgg
    33301 aaaataaact tcccgaaatg acaatcagca ataatattcc tgtcactttt tttgtcggca
    33361 aggaaacatc atattttatt attacagcca aaaaaagaag ggataaaaag gcaaaagcac
    33421 atagataagg gctatacatt cccagcattt tggcaacttc atccggatca ctctgcagca
    33481 cactaatcgc aaatccatca ttgaaagttg taccaaaggt acaccatgaa tagaggctaa
    33541 tactcatgtc tgccgcagtc agtaacacaa atggtgcggc aacaagcagg cgaagcggaa
    33601 gggatgatat acgtttaaca accaggatgg tcagaacgaa gaacagcaat cgctgaacca
    33661 tatgcgctcc ggaagaggcg actaagttcg taataccaaa aacaataaca ctaagataca
    33721 acaaccaatg agatttaaaa cttctagcaa atttgttgaa tactgtcatc caga
//
LOCUS       DBFIFM010000030        32674 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.030, whole genome shotgun sequence.
ACCESSION   DBFIFM010000030 DBFIFM010000000
VERSION     DBFIFM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.030"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            356..922
                     /gene="mepS"
                     /locus_tag="N7C74_001270"
     CDS             356..922
                     /gene="mepS"
                     /locus_tag="N7C74_001270"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HGU2431169.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            1103..2659
                     /gene="pdeN"
                     /locus_tag="N7C74_001271"
     CDS             1103..2659
                     /gene="pdeN"
                     /locus_tag="N7C74_001271"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HGU2431170.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCKQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKANIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            2741..4555
                     /gene="yejA"
                     /locus_tag="N7C74_001272"
     CDS             2741..4555
                     /gene="yejA"
                     /locus_tag="N7C74_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGU2431171.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYSGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDHSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            4556..5650
                     /gene="yejB"
                     /locus_tag="N7C74_001273"
     CDS             4556..5650
                     /gene="yejB"
                     /locus_tag="N7C74_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HGU2431172.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            5650..6675
                     /gene="yejE"
                     /locus_tag="N7C74_001274"
     CDS             5650..6675
                     /gene="yejE"
                     /locus_tag="N7C74_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2431173.1"
                     /translation="MSRLNPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            6677..8266
                     /gene="yejF"
                     /locus_tag="N7C74_001275"
     CDS             6677..8266
                     /gene="yejF"
                     /locus_tag="N7C74_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HGU2431174.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIDVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(8270..8614)
                     /gene="yejG"
                     /locus_tag="N7C74_001276"
     CDS             complement(8270..8614)
                     /gene="yejG"
                     /locus_tag="N7C74_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HGU2431175.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALGDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(8947..10137)
                     /gene="bcr"
                     /locus_tag="N7C74_001277"
     CDS             complement(8947..10137)
                     /gene="bcr"
                     /locus_tag="N7C74_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HGU2431176.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(10165..10860)
                     /gene="rsuA"
                     /locus_tag="N7C74_001278"
     CDS             complement(10165..10860)
                     /gene="rsuA"
                     /locus_tag="N7C74_001278"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HGU2431177.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            11009..12769
                     /gene="radD"
                     /locus_tag="N7C74_001279"
     CDS             11009..12769
                     /gene="radD"
                     /locus_tag="N7C74_001279"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGU2431178.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            12894..13178
                     /gene="rplY"
                     /locus_tag="N7C74_001280"
     CDS             12894..13178
                     /gene="rplY"
                     /locus_tag="N7C74_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HGU2431179.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNIQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(13317..14324)
                     /gene="yejK"
                     /locus_tag="N7C74_001281"
     CDS             complement(13317..14324)
                     /gene="yejK"
                     /locus_tag="N7C74_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HGU2431180.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            14506..14733
                     /gene="yejL"
                     /locus_tag="N7C74_001282"
     CDS             14506..14733
                     /gene="yejL"
                     /locus_tag="N7C74_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HGU2431181.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            14753..16513
                     /gene="yejM"
                     /locus_tag="N7C74_001283"
     CDS             14753..16513
                     /gene="yejM"
                     /locus_tag="N7C74_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HGU2431182.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            16588..16664
                     /locus_tag="N7C74_001284"
     tRNA            16588..16664
                     /locus_tag="N7C74_001284"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16622..16624,aa:Pro,seq:ggg)
     gene            complement(16765..19356)
                     /gene="yejO"
                     /locus_tag="N7C74_001285"
     CDS             complement(16765..19356)
                     /gene="yejO"
                     /locus_tag="N7C74_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HGU2431183.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGG
                     LAIAVDQKAGGAIKASTRVMEVFGTNRLGQFEIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     LRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMDESVTRS
                     SVTASAENFTTLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNVDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTRGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            19676..20323
                     /gene="narP"
                     /locus_tag="N7C74_001286"
     CDS             19676..20323
                     /gene="narP"
                     /locus_tag="N7C74_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HGU2431184.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLHEREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(20358..21410)
                     /gene="ccmH"
                     /locus_tag="N7C74_001287"
     CDS             complement(20358..21410)
                     /gene="ccmH"
                     /locus_tag="N7C74_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HGU2431185.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDATLGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(21407..21964)
                     /gene="dsbE"
                     /locus_tag="N7C74_001288"
     CDS             complement(21407..21964)
                     /gene="dsbE"
                     /locus_tag="N7C74_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HGU2431186.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(21961..23904)
                     /gene="ccmF"
                     /locus_tag="N7C74_001289"
     CDS             complement(21961..23904)
                     /gene="ccmF"
                     /locus_tag="N7C74_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HGU2431187.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(23901..24380)
                     /gene="ccmE"
                     /locus_tag="N7C74_001290"
     CDS             complement(23901..24380)
                     /gene="ccmE"
                     /locus_tag="N7C74_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HGU2431188.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(24377..24586)
                     /gene="ccmD"
                     /locus_tag="N7C74_001291"
     CDS             complement(24377..24586)
                     /gene="ccmD"
                     /locus_tag="N7C74_001291"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HGU2431189.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(24583..25320)
                     /gene="ccmC"
                     /locus_tag="N7C74_001292"
     CDS             complement(24583..25320)
                     /gene="ccmC"
                     /locus_tag="N7C74_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HGU2431190.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(25362..26024)
                     /gene="ccmB"
                     /locus_tag="N7C74_001293"
     CDS             complement(25362..26024)
                     /gene="ccmB"
                     /locus_tag="N7C74_001293"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HGU2431191.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(26021..26644)
                     /gene="ccmA"
                     /locus_tag="N7C74_001294"
     CDS             complement(26021..26644)
                     /gene="ccmA"
                     /locus_tag="N7C74_001294"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HGU2431192.1"
                     /translation="MGMLEVRELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAV"
     gene            complement(26657..27259)
                     /gene="napC"
                     /locus_tag="N7C74_001295"
     CDS             complement(26657..27259)
                     /gene="napC"
                     /locus_tag="N7C74_001295"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HGU2431193.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(27269..27718)
                     /gene="napB"
                     /locus_tag="N7C74_001296"
     CDS             complement(27269..27718)
                     /gene="napB"
                     /locus_tag="N7C74_001296"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HGU2431194.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(27715..28578)
                     /gene="napH"
                     /locus_tag="N7C74_001297"
     CDS             complement(27715..28578)
                     /gene="napH"
                     /locus_tag="N7C74_001297"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HGU2431195.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(28565..29260)
                     /gene="napG"
                     /locus_tag="N7C74_001298"
     CDS             complement(28565..29260)
                     /gene="napG"
                     /locus_tag="N7C74_001298"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HGU2431196.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLVVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(29267..31753)
                     /gene="napA"
                     /locus_tag="N7C74_001299"
     CDS             complement(29267..31753)
                     /gene="napA"
                     /locus_tag="N7C74_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HGU2431197.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQDVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(31750..32013)
                     /gene="napD"
                     /locus_tag="N7C74_001300"
     CDS             complement(31750..32013)
                     /gene="napD"
                     /locus_tag="N7C74_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HGU2431198.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(32003..32497)
                     /gene="napF"
                     /locus_tag="N7C74_001301"
     CDS             complement(32003..32497)
                     /gene="napF"
                     /locus_tag="N7C74_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HGU2431199.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            32606..>32674
                     /locus_tag="N7C74_001302"
     CDS             32606..>32674
                     /locus_tag="N7C74_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2431200.1"
                     /translation="MHTPIEVKPVAGSKEWREAWQKR"
BASE COUNT         7934 a         8983 c         8069 g         7688 t
ORIGIN      
        1 cgcaccgtgc tttcatccat cgaaagcaaa aatcattctc taagtaaatg aatggattgc
       61 atgcatttca ctcaatcgta ctttaattga ccaatcccgc ttattaactt tctgtatcac
      121 tttttcttat aaaaaatcat gtaaaaccgc tcgccaagac cgcaccaatc gggtaatctc
      181 gaactcgttt tgcctcggcg gtagattatc ctcacagcat ataattttgt gcgttagtcc
      241 acagatttgg ccttaaggaa ttgtttcaac atgccctggt aattagtctc gtgtcgcttg
      301 gcattttttt tataacgata tttgtcgtta aggacttcaa gggaaaacaa acaacatggt
      361 caaatctcaa ccgattttga gatatatctt gcgcgggatc cccgcgattg cagtagcggt
      421 tctgctttct gcatgtagtg caaataacac cgcaaagaat atgcatcctg agacacgtgc
      481 agtgggtagt gaaacatcat cactgcaagc ttctcaggat gaatttgaaa acctggttcg
      541 taatgtcgac gtaaaatcgc gaattatgga tcagtatgct gactggaaag gcgtacgtta
      601 tcgtctgggc ggcagcacta aaaaaggtat cgattgttct ggtttcgtac agcgtacatt
      661 ccgtgagcaa tttggcttag aacttccgcg ttcgacttac gaacagcagg aaatgggtaa
      721 atctgtttcc cgcagtaatt tgcgtacggg tgatttagtt ctgttccgtg ccggttcaac
      781 gggacgccat gtcggtattt atatcggcaa caatcagttt gtccatgctt ccaccagcag
      841 tggtgttatt atttccagca tgaatgaacc gtactggaag aagcgttaca acgaagcacg
      901 ccgggttctc agccgcagct aataaaccgt ttggatgcaa tcccttggct atcctgacga
      961 gttaactgaa agcactgttt aggcagtgct tttttgtttt cattcatcag agaaaatgat
     1021 gtttccgcgt cttgatccag gctatagtcc ggtcattgtt atcttttaaa tgttgtcgta
     1081 atttcaggaa attaacggaa tcatgttcat acgcgctccc aattctggac gtaagctcct
     1141 gcttacctgc attgttgcag gcgtaatgat tgcgatactg gtgagctgcc ttcagttttt
     1201 agtggcctgg cataagcacg aagtcaaata cgacacactg attaccgacg tacaaaagta
     1261 tctcgatacc tattttgccg acctgaaatc cactactgac cggctccagc cgctgacctt
     1321 agatacatgc aagcaagcta accccgaact gaccgcccgc gcggcgttta gcatgaatgt
     1381 ccgaacgttt gtgctggtga aagataaaaa aacattctgt tcatctgcga ccggtgagat
     1441 ggacattcca ctcaatgaat tgattcctgc gctcgacatt aataaaaatg tcgatatggc
     1501 gatcttaccc ggcacgccga tggtgccgaa caaacccgca atcgtcatct ggtatcgcaa
     1561 ccctttgctg aaaaatagcg gcgtctttgc cgctctgaat ctcaacctga cgccttctct
     1621 cttttatagc tcacggcagg aagattacga tggcgtcgcc ctcattattg gcaatactgc
     1681 gctatctacc ttttcttcac gtttgatgaa cgttaacgaa ttaaccgaca tgccagtccg
     1741 tgaaactaaa attgcgggca ttcctctgac cgttcggctt tatgcagatg actggacatg
     1801 gaacgatgtg tggtacgcat ttttactggg cggcatgagt ggaactgtcg ttggcctgct
     1861 ctgctattac ctgatgagcg tacgtatgcg ccccggcaga gaaatcatga ccgccatcaa
     1921 gcgcgaacaa ttttacgtgg cgtatcaacc ggtggtggat acacaagctt tgcgagtaac
     1981 gggcctggaa gtactgctac gctggcggca tcctgtcgcg ggagaaattc ccccggatgc
     2041 cttcattaac tttgccgaat cgcaaaagat gattgtgccg ctgactcagc acctgtttga
     2101 gttaattgcc cgcgatgccg cagaattaga aaaagtgctg ccggtaggcg tcaaatttgg
     2161 cattaacatt gcgccggacc atctgcacag cgaaagcttt aaagcaaata tccagaaact
     2221 gctcacttcc ctacccgcac accatttcca gattgtgctg gaaattaccg agcgcgatat
     2281 gctgaaagag caagaagcca cacaactctt cgcctggctg cactcggtcg gcgtagaaat
     2341 tgctattgat gacttcggca ccgggcacag cgcgcttatc tatcttgagc gttttacgct
     2401 cgattatctg aaaattgacc gtggatttat caacgccatc ggtacggaaa cgatcacttc
     2461 acccgtactt gacgcggtgc tgacgctggc gaaacgcctc aatatgctga cggttgctga
     2521 gggggtcgaa acgccggaac aggcgcgatg gctaagcgaa cgcggcgtta atttcatgca
     2581 aggctactgg attagtcgcc cgttaccgct ggacgatttt gtccgctggc tgaagaaacc
     2641 gtatacgccg cagtggtaag gtgtgcttac gtcccttatt attcatagtg aaagcatgcc
     2701 ggattgcggc taatgatgag taaaaggaaa tccgttgcag atgattgtgc gcatactgct
     2761 gctgtttatc gctctgttca cctttggtgc gcaggcgcag gctatcaagg aaagctatgc
     2821 ttttgccgtg ctgggcgaac cccggtacgc gtttaatttc aaccattttg attatgtgaa
     2881 ccccgccgcg ccaaaaggtg ggcagataac gttgtcagcc ctcggtacct tcgataattt
     2941 caaccgctat gcactgcgcg gcaacccggg cgcacgcacc gagcagctgt acgacacgct
     3001 atttacgact tccgatgacg aaccaggcag ttattacccg ctgattgctg aaagcgcacg
     3061 ctatgctgac gattattcct gggtggaggt cgctattaat cctcgcgccc gttttcatga
     3121 tggttcgccc attactgccc gcgatgtaga gtttactttt caaaaattta tgactgaagg
     3181 cgtgccgcaa tttcgtctgg tctacaaagg caccaccgtc aaagccattg cgccgttaac
     3241 cgtgcgcatt gagttagcta aacccggcaa agaagatatg ttgagtctgt tttcgctgcc
     3301 ggtatttcca gaaaagtact ggaaggatca caaacttagc gatccgctcg ccacgcctcc
     3361 gcttgccagt ggtccgtacc gaattacgtc ctggaaaatg gggcaaaata ttgtctattc
     3421 ccgcgtgaaa gattactggg cagcaaactt accggtaaac cgtggacgct ggaatttcga
     3481 caccattcgc tacgattatt acctcgatga taatgtcgcc tttgaagcgt ttaaagcagg
     3541 tgcctttgat ttgcgtatgg aaaacgacgc caaaaactgg gccacacgct atagcggtaa
     3601 aaatttcgat aaaaaataca tcatcaaaga tgagcaaaag aacgaatcag cccaggatac
     3661 gcgctggctg gcgtttaata tccaacgtcc ggtattcagc gatcgccggg tccgggaagc
     3721 aatcaccctc gcctttgact ttgaatggat gaacaaggcg ttgttttaca atgcctggag
     3781 tcgcacgaac agttattttc agaataccga atacgcggcc agaaattacc ccgacgccgc
     3841 ggagctggtg cttctggcac caatgaaaaa agatttaccg ccagaagtct tcacacaaat
     3901 ctaccagccg ccggtatcga aaggcgatgg ctacgatcgt gacaacctgt taaaagccga
     3961 caaacttctc aacgaagcgg gctgggtgct gaaaggtcag caacgcgtta atgccactac
     4021 gggtcagccg ctcagctttg aattattgct tcctgcaagc agcaatagtc agtgggtatt
     4081 gccgttccag cacagcctgc aacggctggg tatcaacatg gacattcgca aggtggatca
     4141 ctcgcaaatc accaaccgca tgcgcagtcg cgactatgac atgatgccgc gcctatggcg
     4201 ggcgatgccg tggcccagtt ccgatttaca gatttcctgg tcatcggaat atatcaattc
     4261 cacttataat gcccccggcg tgcaaagccc ggttatcgac tcgctgatca accaaattat
     4321 tgccgcgcag ggaaataaag aaaaattact gccgttaggg cgagcactgg atcgcgtatt
     4381 aacgtggaat tattacatgc tgccaatgtg gtacatggcg gaagaccgtc tcgcctggtg
     4441 ggataaattc tcccaacccg ctgtgcgccc tgtttacagt ctgggtatcg atacctggtg
     4501 gtatgatgtt aataaagcgg ccaaactgcc gtcagccaga caacagggag agtagatggg
     4561 cgcttatctg attcgccgtc tgttgctggt gatcccaacg ttatgggcga ttatcactat
     4621 caactttttc atcgtgcaaa ttgcgcctgg cggtccggtc gaccaggcca tcgccgccat
     4681 tgagtttggt aatgccggag tattacccgg cgcaggcggt gaaggcgttc gcgccagcca
     4741 tgcgcaaacg ggcgtcggta atatcagcga cagtaattac cgtggcggac gcggattaga
     4801 tccagaagtg atcgccgaga tcactcatcg ctacggtttc gataagccga tccacgaacg
     4861 ttacttcaaa atgctctggg actacatccg ctttgatttt ggcgatagcc tgtttcgcag
     4921 cgcctcggtg ctgacgctga ttaaagacag tctgccggtt tccatcaccc tcggattgtg
     4981 gagcacgctg attatctatc tggtgtcgat tccgttaggc attcgcaaag ctgtttataa
     5041 tgggagccgc tttgacgtct ggagtagcgc atttatcatc atcggctacg ccattccggc
     5101 ctttttgttt gccatcctgc tgattgtctt cttcgcaggc ggcagctatt tcgacctgtt
     5161 ccctctacgc ggcctggttt ccgctaactt tgattcgctg ccgtggtatc agaaaatcac
     5221 cgattatctg tggcatatca cgctgccggt gctggcgacg gtgattggtg gctttgcggc
     5281 gctgactatg ctgacaaaaa actcattcct tgatgaagta cgcaagcaat acgtggtgac
     5341 cgcgcgtgcg aaaggggtaa gtgaaaaaaa tattctctgg aaacatgtgt tccgcaacgc
     5401 catgctgctg gtgattgccg gttttccggc gacgtttatc agcatgtttt ttaccggctc
     5461 gctgctgatt gaggtgatgt tttcactcaa tggtcttggc ttactgggct acgaagcgac
     5521 cgtctcgcgc gattatcctg taatgtttgg taccttgtat attttcaccc tgattggcct
     5581 gctgctgaat attgtcagtg atatcagcta tacgctggtc gatccgcgta tagattttga
     5641 gggacgttaa tgtcgcgact caaccccgtc aatcaggccc gttgggcgcg ttttcgccat
     5701 aaccgtcgcg gctactggtc gttatggatt ttcctcgttt tgtttggttt gagtttgtgt
     5761 tctgaactta tcgccaacga taaaccgttg ctggtgcgtt atgacggcag ttggtatttc
     5821 ccgttgttga aaaactacag cgaaagcgat tttggcggcc cgctggcaag tcaggctgat
     5881 tatcaggacc cgtggctgaa acaacggctg gaaaataatg gctgggtact gtgggcaccg
     5941 attcgctttg gtgctaccag tatcaacttt gctaccgata agcccttccc ttctcccccc
     6001 tcccggcaaa actggctggg aacggatgcc aacggcggcg atgtgctggc acgtattctc
     6061 tatggcacgc ggatctcggt tctgtttggc ctgatgctga ctctctgttc cagcgtgatg
     6121 ggcgtgctgg cgggggcgct acaaggctat tacggcggta aggtcgatct ctgggggcag
     6181 cgctttattg aagtatggtc ggggatgcca acgctgtttt tgattattct gctttccagc
     6241 gttgtgcaac ctaacttctg gtggctgctg gcaattactg tcttatttgg ctggatgagt
     6301 ctggtcggcg tggtgcgggc ggagttttta cgtacccgca atttcgacta catccgcgcg
     6361 gcacaggcgc ttggcgtcag cgatcgcagt attattctgc gtcatatgtt gccgaatgca
     6421 atggtcgcta ccctcacctt tttaccgttt attttatgta gttcgatcac caccctgacc
     6481 tcactcgatt tcctcggctt cggtctgccg ctcggttcac cgtcactcgg tgaactgctg
     6541 ttacaaggga aaaataacct tcaggctccg tggcttggga tcaccgcctt cttgtcggtg
     6601 gcgatattat tgtctttgct gatctttatt ggtgaagccg tccgcgacgc atttgatcct
     6661 aataaggcgg tgtagcatga cgcaaactct gttagcgatt gaagatttgt cggtgggttt
     6721 tcgccatcag caaaccgtac gtacagtagt caatgatgtt tcactacaga ttgaggctgg
     6781 cgaaacgctg gcgctggtgg gtgagtcagg ttcaggcaaa agcgttaccg cgctgtcaat
     6841 tttacgcctg ctcccttccc cgccggttga atatctctcc ggcgatattc gttttcatgg
     6901 cgaatcgctg cttcatgcca gcgaccaaac gttgcgcggt gtacgcggta ataagatcgc
     6961 catgattttt caagagccga tggtgtcgtt aaatccattg cataccctgg aaaaacagct
     7021 ttatgaagtg ctttcactcc accgcgggat gcgtcgggaa gcggctcgtg gcgaaattct
     7081 taactgcctt gatcgcgttg gtatccgcca ggcggcaaaa cggctgacag attatccgca
     7141 tcagctctcc ggcggcgaac gccagcgggt gatgattgcg atggcgctgt taacgcgacc
     7201 ggaattatta attgccgatg aaccgaccac cgcactggac gtctctgtcc aggcgcagat
     7261 tttacagctg ttgcgcgaac tgcaaggcga gttgaatatg ggcatgctgt ttattactca
     7321 taacctcagc attgtcagaa aactggccca ccgcgtggcg gtaatgcaaa acggtcgctg
     7381 tgtcgagcaa aattacgccg ctacgctatt tgcctcaccc actcatcctt acacacaaaa
     7441 gctactcaac agtgaaccgt caggcgatcc agtgccgttg ccagaacctg cctcaacgtt
     7501 gctggatgtt gaacagcttc aggttgcctt ccccattcgc aaagggattt tgaagcgcat
     7561 tgtggatcat aatgtggtgg tgaaaaacat cagttttacg ctacgagcgg gtgaaacact
     7621 gggtttagtg ggcgagtccg gttccgggaa aagcacgacg ggactggcgc tgctgcgact
     7681 gattaattct cagggcagca tcatctttga cggtcagcca ctgcaaaatt taaatcgccg
     7741 ccagctgtta cctattcgtc atcgcattca ggtggtattt caggatccaa actcctcgct
     7801 caacccacga ctcaacgttt tgcagattat tgaggaaggc ttacgggttc accagccgac
     7861 gctttctgcc gcacaacgcg aacaacaagt gatagacgtg atgcatgaag tgggattaga
     7921 tcctgaaaca cgccaccgtt atccggcgga gttctctggt ggtcagcgac aacgtattgc
     7981 gattgccagg gcattaattc ttaagccctc gctgatcata cttgatgaac cgacatcatc
     8041 actcgacaaa acggtacagg cgcaaatatt gacgctattg aaatcattgc aacaaaagca
     8101 tcaactggcc tatttgttta tcagccacga tttgcacgtt gtccgcgcgt tatgtcatca
     8161 ggttatcgta ctgcgacaag gggaagtagt ggaacaagga ccgtgcgcgc gcgtgtttgc
     8221 cacaccgcag caggagtata cgcgtcagct actggcgttg agctgacgct taaaaaggat
     8281 tgtagtttga aaagggttcg gcaattgcca caccaaaatt tttcaatcgg catgttgcag
     8341 caaactcgtc ctggcgattt acaaacaggc acggctcacc ttcacactcc agcaccgaac
     8401 atggaacttc gatgtcgcct aacgcctggc ttagtttata catcaccgac catgcattat
     8461 caccatccgg gctaagcaat ttaagcgcca ccaggctgtt gtcaccgcac ggtccatttt
     8521 gcggaatcgg ttcaaccttc gaacctgcga aaccagcaga ccagcgatag ttctgcggca
     8581 gtcgatggac gattgagagt tgtaatgaag tcacttattt tccccggaag cacatttact
     8641 tcacaatttg tttacatcaa ttttaacaca tcatcaacaa tccgtcttta tacagatcgt
     8701 aaagattgat gcctcgcctg cgtggcctct atggctctga tttaagtaat atctgcgggc
     8761 gtggtcacgt ttgcggggct atatgtaccc gtttctgcga tctaactcaa cctttttaac
     8821 tacaatgatg tgacttttta cataaattga ttttacataa aataaacata tatcggggga
     8881 atgatagatt tgtggttgac atgcatcaac aaaggaagcc ttttagcttc ctcgttgtgc
     8941 aatagatcac cgttttttcg gccgactggc gtacagacag aagagaatgg agctggttgc
     9001 gcagaatgca attgaccaaa tcatcggcca tgcagagtta aaggtcgcaa gagaaagcaa
     9061 tgcgccaaca attgccccta tgccaaaacg gaaggttcct gccagcgaag atgccgttcc
     9121 cgccatatgg ggaaactcat caagaatgac cgccatcgca ttggatgaca ccatcgacac
     9181 gcagcccaca aacgccgcaa cgccaaccac cagcgaccaa aatcccagac ccagcagcgc
     9241 actgatgacc atccacgctg ccataataaa ttgtatccac aaccccgagc ggaacatatt
     9301 taacgcgcca atacggcgga cgaagcggct attaaagatg gtcatcacga acaggaaaac
     9361 aatgtttagt gcaaagtaat aaccaaagtt ttccggcgcg acgtggttaa tttcaatgta
     9421 gacaaacggt ccggcgctta agaacgagaa catcccggca aagctgaaac cactggccag
     9481 catataactc aggacacgct tatgacggaa cagcgccgca aagttaccaa tagtggtacg
     9541 aatgtgaaaa ggctggcgac gctccggtgg taaggtttct ttgatcagaa agaagatcat
     9601 tgccgaagcc agaatcgccg ccacggcgag gatccagaag atgtaatgcc agctcagcca
     9661 caccagcacc cagccgccaa ctatcggtgc catcagcggt gcaatggtcg tcaccagcat
     9721 gacaaacgac atcatccgcg agaactcttc tttcgggtag atatcacgca tcaaagcgtt
     9781 gataaccacg ctggccgcag ccgccgccag cccgtggaag aaacgcatca caatcagctg
     9841 atcgatggtt tgtgccaacg cacacgccac cgcggcggcg gcaaacacca gcgtaccgcc
     9901 aagaaccacc ggcttacgcc cgaagctgtc tgccatcggc ccgtagatta actgccccaa
     9961 cgcaaagccc agaatataag tactgagggt catctgcgta ctgcccgccg gtacgccaaa
    10021 ctgcgctgaa attaccggta gcgcgggcag atacatatca atcgacagcg gcatcaacat
    10081 ggccagcagg ccaaggataa aaacaatagc aaacgacgaa tgctgtcggg tggtcacaac
    10141 gggctcctga aagtcattga aaagttagac gacgctggca atttcttctt cagttaacgg
    10201 acgatattca ccgggggcta aatcagcatc cagcgtaatg ccgccaatac gttcacgatg
    10261 cagctcaacc acatggttac ccacggcggc gaacatgcgt ttcacctgat gataacgccc
    10321 ttcgctgatg gtcagacgaa cctgcgttgg ggtaatcact tcaagcaccg ctggcttagt
    10381 gagatctttt tcgttatgca gctgcacgcc tttagcaaat tgctctgccg tatcgtcagc
    10441 tacgggtgat tccagcgtca ccagataggt cttctcgcaa tgatggcgcg gagaagtaat
    10501 gcggtgcgac cactgaccat catctgtcat tagtaccaga ccggtggtat cgatatccag
    10561 ccgccctgcg gcgtgcagtt tccacgccac tggttcatct agaaaataga gcaccgttgg
    10621 gtgatcaggg tcgtccgtgg agcaaacata gccctgaggc ttattgagca tgaagtaacg
    10681 tggaccgtgt tgctgcgcca gcgggttgcc atcgtaagcg acatcatgtt caggaagcag
    10741 tttgaacgct gcattacgga cgatttcgcc atcgacggtg acacgattgc cgcggatttc
    10801 acgcccggca atagcacggc taacgccgag ttgctgtgcg ataaatttat caagtcgcat
    10861 gtgtgtgatt ttgcctgtaa aaacggaggt cgggcattga cccgaaaatc tgaactgttg
    10921 tctgcccagt atagcggtct aataacttcc ctcaagggaa aaagaaccgt ggcatactat
    10981 tagcagaata atctacctac gcgagaccat gatttttaca ctccgcccat atcagcaaga
    11041 agccgtggat gccacgctca accattttcg tcgtcataaa acccctgccg ttatcgtgct
    11101 gcccaccggt gcaggtaaaa gcctggtgat agcggaactt gcgcggctgg ctcgtggtcg
    11161 cgtgctggtg ctggcacacg ttaaagaact ggtggcgcaa aaccatgcaa agtatcaggc
    11221 gctggggctg gaagccgata tttttgccgc cgggctaaag cgcaaagaga gccacggtaa
    11281 agtggtattt ggcagcgtgc agtcggtcgc ccgtaatctt gatgcctttc agggtgaatt
    11341 ttcgctgttg attgtcgatg aatgtcaccg tattggtgac gatgaagaga gccagtatca
    11401 gcaaatcctc actcacctga caaaagtgaa tccccactta cgcctgctgg ggctgactgc
    11461 cacgcctttt cgactgggca aaggctggat ctaccagttc cattatcacg gcatggtacg
    11521 cggcgatgag aaagcccttt tccgtgactg catttatgaa ctgccgctgc gttatatgat
    11581 taaacacggc tatctgacgc cgccagaacg actggatatg ccagtagtgc aatacgattt
    11641 cagccgcttg caggcacaga gtaacgggct attcagcgaa gccgatctca accgcgagct
    11701 aaaaaaacaa caacgtatta ccccgcacat catcagccag attatggagt ttgctgcaac
    11761 gcgcaaaggg gtgatgattt tcgccgccac cgttgaacac gcaaaagaga ttgtgggatt
    11821 actacccgcc gaagatgctg cactgattac tggcgacacc cccggcgctg agcgcgatgt
    11881 gttaattgaa gattttaaag cccagcgttt tcgctatctg gtcaacgtcg cggtactgac
    11941 caccggattt gacgccccgc acgtcgatct tatcgccatt ctgcgcccta ccgaatcagt
    12001 gagtctttac caacaaattg tcgggcgcgg tctgcgtctc gctcctggca agactgattg
    12061 cttaattctt gattatgcgg gtaatcctca cgatctctac gcgccggaag ttggtacacc
    12121 gaaaggcaaa agtgacaacg ttccggtaca ggttttctgc cctgcctgcg gttttgccaa
    12181 caccttttgg gggaaaacga ccgccgacgg gacattgatt gaacactttg gtcgccgctg
    12241 tcagggatgg tttgaagatg acgacggtca tcgcgaacag tgtgacttcc gtttccgttt
    12301 taaaaattgc ccgcaatgta acgcagaaaa tgatattgcc gctcgccgct gccgcgaatg
    12361 tgacacggta ctggttgatc cggatgatat gttaaaagcg gcgctacgac tgaaagatgc
    12421 gctggtatta cgctgtagcg gcatgtcttt gcagcatggg cacgacgaga aaggcgaatg
    12481 gttgaaaatc acctattacg atgaagacgg cgcggatgtg agtgagcgtt tccgtctgca
    12541 aacgcccgcc cagcgaactg ccttcgagca gctttttatc cgcccgcata cgcgcacacc
    12601 gggcatccct ctgcgctgga tcaccgccgc cgatatcctc gcccagcaag ccttattgcg
    12661 acacccggat tttgtcgttg cccgcatgaa aggtcagtac tggcaagtgc gtgaaaaagt
    12721 gttcgattac gaaggtcgtt ttcgtcgggc gcacgaatta cgcggttaat ggtagttttg
    12781 attgatgtat aaggcgattg agtatagaat ctcgcccgct tttgcatacg caaagcagat
    12841 cacttacctg ttgctgggtc gcctgtagca ggattaattt aagagagaaa gaaatgttta
    12901 ctatcaacgc agaagtacgt aaagagcagg gtaagggtgc gagccgccgc ctgcgtgccg
    12961 ctaacaagtt cccggcaatc atctacggtg gcaaagaagc accgctggct atcgagctgg
    13021 atcacgacaa agtcatgaac attcaagcta aagctgaatt ctacagcgaa gttctgacca
    13081 tcgttgttga cggtaaagaa atcaaagtta aagctcagga cgtacagcgt cacccgtaca
    13141 aaccgaagct gcagcacatc gacttcgttc gcgcttaatt gctgaataag ttgtaaaaaa
    13201 ccccgctccg gcggggtttt ttgtatctgc agattatgcc tgattacgat attgctattt
    13261 tttgcaggcc agataaggcg ttcacgccgc atctggcatt cataacacaa aaccgattaa
    13321 ttgccgccgg acgtccggcg ctgcaattga tcgcgcaaat tcggcggcgt gcctttaata
    13381 gtcagggtat cggtcgccgg atcccagaaa atccgttcgc ccaacagcat cgcatcaaaa
    13441 ttaatagtca agccaccgcc gctaccggca aacttcgtca actgacgcag tgtgctacgg
    13501 tctgccggga agctctcttc cagttcgtag cctttttcag ccgcaaactc cgtgaagctc
    13561 acttcgctga cgcctgccag ctctttcgac agcgattcca gttcgatctc ttcgcctgcc
    13621 tgcagctgct cattacagta gctataaacc tgctggcgca cgttctgtcg ctctgcttta
    13681 tccagttgtg cttcggcagt gaaatcatca accgcttgca acagcccacg gttttgcgct
    13741 ttcgcgttca atccttcgct ggccccgagg aaatccataa agaaatccgc cactttgcgc
    13801 cctactcgcc ctttcaggaa agtgagatag cgggtcgact ctggattggt ttcccattcg
    13861 gttaaatcga tacgcgcaac aatatccgca tgattgatat caagataatg ggttgggttg
    13921 atgtcgagat tttcgttaac gcgcatactg ctcaggttgc tcaggactgc caccagcaga
    13981 tactccaccg ccagataacg gtagtggcaa aacagcacga atccaccgtc agcaaaagga
    14041 tatttcgcta attcgtcacg caggcgaccg gttgccgcgc ggctaaatgc cagaaagtcc
    14101 tcttcaccct gacgctgtaa gcgcagcgtc tgcgccagtt cactctcttc gctaaacaag
    14161 ccgtaagctt tatttttggc gctatagacc cgatgcagtt ctgctaccat ctcaacgacg
    14221 gtttctgtcg gttccagcaa tgaatcgcgc aacaccagct caaggttctg ctcatcacgc
    14281 ttgataagct ggtgcagagc aatctggttg atatccagac tcatgataaa ctctccttta
    14341 agaccgggcg gtattcaacc accgccggtt aagcgacgca ataaaagatg gcggtcattt
    14401 tacagaaggc ggcgtaacga gaatgattta acgcataaaa aagcagaaaa aaaaccgttg
    14461 ctacggtaat atgttgccct ttcatcaact aactgatttc gatttatgcc acaaatttcc
    14521 cgctacagtg atgaacaggt tgaacaattg ctcgcagagc tgctcaacgt actggaaaaa
    14581 cataaggctc cgaccgacct ttccctgatg gtgttaggca acatggtcac taaccttatc
    14641 aacaccagca ttgccccggc ccaacgccag gcaattgcca actcttttgc ccgcgcctta
    14701 cagtcctcta tcaacgaaga caaagcgcac taagggaaac agataacagg ttatggtaac
    14761 tcatcgtcag cgctaccgtg aaaaagtctc ccagatggtc agttgggggc actggtttgc
    14821 actgttcaat attctgcttt cgctcgtcat tggcagccgt tacctgttta tcgccgactg
    14881 gccgaccacg cttgctggtc gcatttattc ctacgtaagc attatcggcc atttcagctt
    14941 cctggtgttc gccacctact tgctgattct cttcccgctg acctttatcg tcggctccca
    15001 gaggctgatg aggtttttgt ccgtcattct ggcaacggcg ggaatgacgc tattactgat
    15061 cgatagcgaa gtctttactc gtttccatct ccatcttaat cccatcgtct ggcaactggt
    15121 tattaaccca gacgaaaatg agatggcgcg cgactggcag ctgatgttca tcagcgtgcc
    15181 ggttatttta ttgcttgaac tggtgtttgc gacgtggagc tggcaaaagc tgcgcagcct
    15241 gacgcgtcgt cggcgcttcg cgcgtccgct ggccgcattc ttatttatcg cctttatcgc
    15301 ctcgcatgtg gtgtatatct gggccgatgc caacttctat cgccctatca ccatgcagcg
    15361 cgctaacctg ccgctttcgt acccgatgac ggcgcgacgt tttcttgaga agcatggcct
    15421 gcttgatgcg caggagtatc aacgccgtct tattgagcaa ggtaatccag acgccgtttc
    15481 cgttcagtat ccgttaagcg aactgcgcta tcgcgatatg ggcaccgggc agaatgtctt
    15541 gttgattact gtcgatggcc tgaactactc acgcttcgag aagcagatgc ctgcgctggc
    15601 aggttttgct gagcaaaata tttcgttcac gcgccatatg agctccggca acactacaga
    15661 caacggcatc tttggcctgt tctatggcat ctcgccgagc tatatggacg gcattctgtc
    15721 gacccgtacg cctgcggcat taattactgc gcttaatcag caaggctatc agctggggtt
    15781 attctcatca gatggcttta ccagcccgct gtatcgccag gcattgttgt cagatttctc
    15841 gatgccgagc gtacgcaccc aatccgacga gcagaccgcc acgcagtgga tcaactggct
    15901 gggacgctac gcacaagaag ataaccgctg gttctcgtgg gtctctttca atggtactaa
    15961 cattgacgac agcaatcagc aggcatttgc acggaaatat agccgggcgg caggcaatgt
    16021 cgatgaccag atcaaccgcg tgctcaatgc actgcgtgat tctggcaaac tggacaatac
    16081 ggtggtgatt atcactgccg gtcggggtat tccactgagc gaagaggaag aaacctttga
    16141 ctggtcccac ggtcatctgc aggtgccatt agtgattcac tggccaggca cgccggcgca
    16201 gcgtattaat gcgctgactg atcataccga tctgatgacg acgctgatgc aacgcctgct
    16261 acatgtcagc acacctgcca gcgaatattc gcaaggtcag gatttgttca accctcaacg
    16321 ccgtcattac tgggttaccg cagcggataa cgatacgctg gcaattacca ccccgaaaaa
    16381 gacgctggtg ctgaacaata acggtaaata ccgcacttac aacttacgtg gtgaaagagt
    16441 gaaagatgaa aaaccacagt taagtttgtt attgcaagta ctgacagacg agaagcgttt
    16501 tatcgctaac tgattaatta taaatcagtt agcgaaatat cttacttgca atcggtgtgg
    16561 aaaacggtag tattaacaac cacgagtcgg cacgtagcgc agcctggtag cgcaccgtca
    16621 tggggtgtcg ggggtcggag gttcaaatcc tctcgtgccg accaaaaatc ccaaaaaaac
    16681 caaccgtaaa ggttggtttt tttatatctg catttaattc gataaacaga ccgcgacaca
    16741 tcagggcctg tttattgtct gttatcagaa cgtccagacc acacccgcct gggtattcca
    16801 cggcgattct acccctgcgc cattgccata gctgactgac aaatgaccgc ttaacgtcgg
    16861 ggtaaacgat gaccttatcc cagcctgata aacgccacgg gttccagcca catcattatt
    16921 aaatttgcca tcgtcattaa ctttcacctg gttagaatcg gcgtattcct gacgcacggc
    16981 ggctttcagc cagggttcca gcgtcgtacc gttttgcagg tccatgtgat agcttaccgc
    17041 cgttcccgct tcagcgcgta atatccgggt atttcccaca tccgcgcgca tgccgtttga
    17101 taacgtgtag tcctgaccat ctgtggtaaa gccggtaaag gccagatagg gtctaacact
    17161 ccacaatccg tcaacccaac ggaacccgct ctcgacatga gcacccgcgc cgttactatt
    17221 gtaatcgcca aacgctgttg ccccattact catcttgcca tggatggtgt tggcaaaacg
    17281 gtcaactttc accactccat caacataggc accgttctga tgctcccaac cggcataagc
    17341 ccccagggta tagctatcga cattgccttt gccgccgcga tcaaaaccaa tatcagaatg
    17401 agagtaacca aagaacaagc cgcgaattgt gctgctttct tcacgggaga aacggctatc
    17461 gataccgagc gtcaggcccg tcaatgtttg ctcaaaacca gctcccgcat cagtggtcac
    17521 gttgttgcgg gtgttaattg ccgaactcca catcgccgta tcgtaactaa cgccttttac
    17581 gctaccaaga cgctcacgca cggtgtccag ttctgcatca aataccagcg gttgtgcggc
    17641 cgccatattc agcacatcag tggttgaagg ggtaatttgc gcgcgattct ctgccagact
    17701 ccagctatgg ttaccattat ccagcaaggt atattcatac gtaccgatat caacaacgcc
    17761 tccggcattg cccaacgtaa atgcagcatc accgccgccc gttgttacca gtgtaaggct
    17821 atctcctgct gccgggctgg caccggtgtc cgtcacgaat attttgaaat cacctgttgc
    17881 ctgaccggtg acgttgagct gatcgctctg atgattcgcc atatcggtac gcatataaaa
    17941 attgccgttt cccgacaggg tattggtggt caacgtagtg aaattttccg cacttgccgt
    18001 cacagatgaa cgagtcactg actcatccat cataatcaca tttgcgccag acatcgcaag
    18061 gttggctaag ttaaggcggg tgttttggtt cgtcgtagtg atatcggcaa tgatggtcca
    18121 tgcgctattt tcgagcgaaa catccgcttt gctggtatcc acggcaccat gcgttcttaa
    18181 agaagcgccc tcagaaacca ctaccttacc caccaacatt gcgggtgcat aatttatttg
    18241 cggcttcata acctcaagaa tgccatcatt tcctctgact gacacgttaa ccattgtgcc
    18301 agcccagacg ttagcgcggc cattgttgat aaccatattt tcagagatgg ctttagagga
    18361 atacgttata cttccattct gatctgatcg accgagatca tataccgcat ttgcctgcac
    18421 tctcgtacca gtatcctttc ccagcgattg catagtggca tgctcatcga ggatagtgtc
    18481 aatggcctgc gtgtcctcca taacaataag cccggaacca tcatccagtt cataattttt
    18541 tgacacacca tcttttatac taaattggcc tttactgttt gtaccactca cttccagcgc
    18601 attcgttgag acaattaatt ttccgcctgc ttttttatca acgccagttg cagtcccgcc
    18661 atccataacc tccagtaagc cgccactatc tacagtagta ttataagcga agtcattttc
    18721 ttcaactcgc aaacttccgc cgttttccaa cagcatattg ttagcaatac cattcttgat
    18781 ttcgaactga ccgagacggt ttgttccgaa tacctccatg acccgcgtgc ttgctttaat
    18841 agcaccgcct gctttctgat ctaccgcaat cgctaatccc ccctctttaa cctccagtaa
    18901 tcctcccttt tcgatagcgg caaggacggt aatcccatct ttttcaacga ttaagcgccc
    18961 gcctttaatc gtgggatcat tcgctgcgcc ataaacctgt agcacaccat tattgagcgt
    19021 agtctggttt gccgcgccac ccatttgaac aatttgataa ccatcattta atactgagta
    19081 ttctgcggtg ccattcattt caatgtactg atacccgccg ttaatttcag tattactact
    19141 tataccaaat tctttaatat gctgttctcc gccaggattg attttcgtat tattggcggt
    19201 cccataaacc agctggatat ctttttccag gacaacacca tcaactgtct caccatactc
    19261 aactgttgat gccaatgcta tgggtgaata taacgctgta gctaccagaa cagatattgc
    19321 ggaacgacaa agagaaacag aaccagattg atgcattgag ctttcatcct atgaaattaa
    19381 ttgcagttaa aagcattggg tacagaaaat acccatagct ccatacccgg agtcagtttt
    19441 taaaaactgt ttaaagaaat gcacaagtat tgtgattgat tttttagttg tttttcttga
    19501 tgagaagctg atgcaaaatt ccgtctttat aatgaaaatg atgccaaagc gaacgacaag
    19561 gttgtagttt tcgctacatg tccatacata aaatggggta acattcacgc gcctggtagc
    19621 gttaccaacg ctacgctcaa acataatgat tctaataaaa cctcaggaga ctactatgcc
    19681 tgaagcaacg ccttttcagg tgatgattgt ggatgatcat ccacttatgc gacgcggtgt
    19741 tcgtcagtta ctggagcttg atcctggctt tgaagtggtc gccgaagcgg gcgacggcgc
    19801 gagtgctatc gatctggcga atagactgga tatcgacgtg atcttgctgg atctcaatat
    19861 gaaaggtatg agtggcctgg ataccctcaa tgccttgcgc agggatggcg ttaccgcgca
    19921 aattattatc ctgaccgtat cagatgcctc cagcgatgtc tttgcactga tagacgcagg
    19981 cgcagacggt tatctgttga aagacagcga cccggaagtg ttgctggaag cgattcgtgc
    20041 cggagcgaaa ggcagcaaag tctttagcga acgcgtcaat cagtacttac atgaacgtga
    20101 aatgtttggc gcggaagaag atcccttcag cgtgctgacg gagcgcgagc tggatgttct
    20161 gcacgagctg gcacaggggc tgtcaaataa acagattgcc tcggtgttga atatttccga
    20221 gcagacagta aaagtacata ttcgcaatct actgcgcaaa ctcaatgtcc gctcacgcgt
    20281 ggcagccacc attctgttcc tgcaacaacg cggggcacaa taaaaatagc ccgatggcat
    20341 caccatcggg cctcttttta tttactctcc tgcggcgaca aatgttgcat cgcctgcgcg
    20401 atactacgtt caatcaccgc acggcgagta tcgttggcag gtaagagttt caacatcatc
    20461 tcccacgcgg caacggcttc gccaaatcgc tgctgctcaa aggcattaaa cgcatacatg
    20521 cttaacacac gaatattgct atggtccgtt ctcaccaact ggcgcagcag ttcgccgccg
    20581 aggcggttgt cgttgggatc agatgaacgt gtcaacgctt cagcataacc cagtgtggca
    20641 tcgctgtttt tcggatcgag gcgatacgca gtagcgtatg catcggtggc gatactggcg
    20701 ttacccagcg ccatgccaac gcgacccaac ataatccaac cttctatatc tcccggattt
    20761 ttttgcagtt gagtacgcat ccccagcgca aggcgcgaca tctcttcttc gttgagcgga
    20821 tcggctttcg gatccagcgc cctgtccagc aacgccggag cctgtgccgt ggcctgctgc
    20881 cagattttca cctgctgata attgccagtc tggtagtagc tgacgccagc cacaattaac
    20941 gccaccacaa tacccggcag ataaacaaca tatccggcac gcttaccttc cggcacgctt
    21001 tgttcaggaa acgcttccgg taccacgcgt acccgacgcc gcgaacgggc gtatatgacc
    21061 caaccgccaa taccaatagc cactaccgga agcacccaca gcagcacggt cagcggcgtt
    21121 aacggcggat cgtaagtgac gaagttgccg taacgcgcca ccatataatc gacaatctct
    21181 ttcttacttt tgccttcctg catcagttca tacactttct ggcgcaggtc ggtggcaatc
    21241 atcgagttgg aatcggcaat gctgttattc tggcatttcg ggcagcgcag ttcttcagtg
    21301 agctgacgga actgttgttc ctgtgcttca tctttaaact gcaacacgtc gatggtcgcc
    21361 agcgctgagc cggagatcat cagcatcagc acgcccaata aaaacctcat tgtgcggcct
    21421 ccttactgta tttctcccac agcggcttga tctcttcttc ccagacgcga ggattgagat
    21481 cgcccgcatg gcgatagcga atgatgccgt tgccgtcaat aagaaacgtt tctggcgcgc
    21541 catagacacc gagatccagc cctaacatgc catcgccatc aaaaaggctt agcgcgtaag
    21601 gattgcccag ctctttcagc cagctgattg ccttctggcg atcgtcttta tagttcatgc
    21661 cgaccacgcg gatgccctgc gcagaaagct gattcagata ttgatgttcc gcacggcagg
    21721 tcggacacca ggtcgcccag acgttaagca gcactggttt gccctgagtc agcacatccg
    21781 cctgataaaa ctgccccgga ttgtccagtg attcgagacg aaacttcggc acaggcttgc
    21841 caatgagcgc cgattccaga ttggtcggat catccccttc ggcattacgc gccagctgcc
    21901 acagcagcgc cgcggcaatc gccaggaaga taatcaacgg aattaacaat actttgcgct
    21961 tcatacagcc tccggcgcag ttttttgcgg gctcacgcgc ttacgatagc gaggatcaaa
    22021 cagacacagc agtccgccca acgccatcat cagcccgccc gcccaaatcc agcgaacaaa
    22081 tggtttgtag taaagacgca cagcccacgc gccgttttcc agctcttcac cgagcgcggc
    22141 gtacaggtca cgcgtgatgc cgccgtcaat tgccgcttcg gtcatcatcg acccggcagt
    22201 gttgtaataa cgtttttccg catacagcac cgtttccggt ttaccctctc gcgttacgcc
    22261 gatagtcgcc acaccgccac gccagttcgg gccagtcacc tctttgacat cacggaaggt
    22321 gaagcgatat tcatgaatat cgacgctatc gccggacttc atgcgcacat cacgctcaac
    22381 gctatagttc tggctaaagg caatgccaac aattgtcact gccagcccca ggtgagccgc
    22441 caccatcccc caataactga aggtggtttt cgtgccgcgt gaaatacgta gcgcagcttc
    22501 cgcaattgcc agcaccgcaa tccagcaggc cattgccagg ccgagcaccg tcatcgccac
    22561 aactttgctt tcgaacagcc acggcaacag cagagacagc accagcgtag agatgaaggc
    22621 gataatcaat aaattgcgga ttttacgtgg gcgatcgcgc ccccagcgca ccagaggacc
    22681 gacacccagc agtagcgcaa acggcaccat cagccaggta aacatggtgt tgaagaacgg
    22741 ttcgccaatc gaaatactgc ccagtcccag ttgcttgtgc accagcggca gcagcgtccc
    22801 cagcaacacc accagcatcg cggcgaccag caaaacgttg ttcgctaaca gcaaagattc
    22861 ccgcgaccac agcgcattgt ttacgcgtga gcgaactttg tgtccacgcg cggcaaacag
    22921 cagcagcgaa ccgccaatca ccagcaccat aaaggcgagg ataaacatac cgcgcgccgg
    22981 atcagacgca aacgcgtgta ccgataccag cacgccggaa cgcacgagga aagtccccaa
    23041 cagacacaac gagaaggcac tgattgccag cagtaatgtc cacgctttga agctggcgcg
    23101 ttgttcagtg accgccagtg agtgcatcag cgcagtcccc accagccacg gcataaacga
    23161 ggcgttttct accggatccc agaaccacca gccaccccag ccgagttcgt aataggccca
    23221 tgcggaaccg agcacgatgc cgagcgtcag gaagatccac gctgccagcg tccacggacg
    23281 agtaaaacgc gcataagtgc tgtccagacg cccgctcagc aaagaagcaa tggcaaaagc
    23341 aaacgccacc gagaaaccca cgtaccccat ataaagcaga ggcggatgga agatcagccc
    23401 cggatcctgt aacagcgggt taagatcgcg cccttcaatc gggaagttcg gcagcgtgcg
    23461 tgagaatggg ttagaggtaa agagaatgaa cagcaaaaag ccgacactga ccatccccat
    23521 tatcgccagt acgcgggcca caatatccag cggaatacgc tgactaaaaa tcgccaccgc
    23581 aaaggtccag ccgctcatca gcagcaccca cagcagcagt gagccttcat gtgcgcccca
    23641 ggtagccgcc acgcgatacc acaccggaag ctgggtattg gagttgctgg caacataggt
    23701 gacggtaaag tcgttgacca cgaaagcatt gaccagcacc agaaatgcgc cagccacaga
    23761 cataaacagc agccaggcaa acaagcggga agacgccatc atgcgcgcat ctccgcgcgc
    23821 cacgccccat agcggataca cggacagcag cagcgcaatt cccagcgcca ggcacagcag
    23881 cccgttacca atttccggca tcatgatgct gggtccttat aaacactcgc cgggcgacgg
    23941 tggttagctt ccatcgcttt ctcaacttct ggcggcgtgt agttttcatc atgtttcgcc
    24001 agcacttctt ttgcgaggat atgattgcct ttttccagct cgccctgcac cacaacgccc
    24061 tgcccttcac ggaacagatc cggcaaaatg ccttcgtaag agacatccac tgagccttca
    24121 gcgtcgtaaa tggtgaaggt cactttcagc gaattgggat cgcgctgcac actacccggc
    24181 atcaccatcc cgccaacgcg cagacgctga ccgacttccg gcatctgctg agtttcacgc
    24241 ttgccgtaga gaatttcccc cggcgtataa aagagatcga tattcgagcg cagcgcatat
    24301 agcaccagac cgatagtcag cgccagccct gccaacacgg cacaggcaat ccacaagcgg
    24361 tttttacggc gaatattcat gcagcctcct gctgttgcgc agcacgtaaa cgcgcctcac
    24421 gcgcccgctg ttgcgccaca ccacgcagaa ttgcgcgatg ctgcatcacc gagtgcacga
    24481 ccaaaaccac cagcggaata acggtcatca ccaccgccag ccagacaaaa aaggcgtaac
    24541 cgcccattgc gaaaaattca ttccaggaag caaatgcagg ggtcatttac ggcctctttt
    24601 cagtatcagt tcactcaccc acggacggcg tttttccatc agcaaaatca aattacgcat
    24661 ccgcatcagc gtcagcgtgg cagagaggag caggaagcca aaaatcgacc agcgcagcgg
    24721 cgaacgcatc gccggatcga tactttgctg catccgcgtt gatccctgat gcagggtgtt
    24781 ccaccactcc acggagtaat gaataatcgg cagattcacc acgccaatca gcaccaggat
    24841 acctgccgca cggcccgcca gacggcggtc gtcgaaggcg tgccacaggg caatcacacc
    24901 cacatacaaa aacagcagca ccagttcaga agtcagacgt gcatcccata cccaccaggt
    24961 gccccacatc ggttttcccc atgcagagcc ggtaaccagg gcaataaagg taaacacggc
    25021 accaatgggg gccatcgccg ccaccgccag gttggccatt ttcatctgcc agacaaggcc
    25081 aataaacgcc gccaccgcca ttgatgcata aatgcccatc gaccagatcg ccgcaggcac
    25141 atgcaggtag ataatgcggt agctatttcc ctgctgataa tcagccggag caaagccgaa
    25201 tccccagatc cagccgacgg taagcacgac tacactggca attgccagcc acggtataaa
    25261 ccagccacag atttgataca gccgtggtgg gatcgccagt tgatgcagtg ttttccacat
    25321 agtttcgata ccagactcga acaaaaatca gtaatccagc gttattgaat gctgattcgt
    25381 aacgctgccg ccgtcgcaaa aggacttaat gtcgcggtgc ctgccagcaa cgcgcctaaa
    25441 attgccagat acccgtcaac gggcaaatgc atagaagccg cgtccatcgc ggcggtggca
    25501 aagatgagta atgggatagt cagcggtaac accagtatgc tgagcagcac accaccgcgc
    25561 ttaagtccca ctgtcagcgc cacgcccggt gcaccgagaa agccaagcgt aggcgttccc
    25621 agcagtagcg ttaacgccat cacttgccag ccataaacat ccattcccag tagcattgct
    25681 accagtggcg aaaggatgag taacggcaga ccggttacca tccagtgcgc catcaccttc
    25741 gccagcacaa cggcgggcaa gggtaacggc aacaacatca attgttcaag actgccgtcc
    25801 tgcaaatcgt cacggaacag tcgttccagc gccagcaagg atgaaagcag cgcggcaacc
    25861 cagataatgc ccggtgcaat acgtgccagc agttgcggct ccggaccgat actgagcgga
    25921 aaaagggtaa ttacaatcag gaagaaccac agcgggttgg cgatttcagc gctatggcga
    25981 aacgctacgc gcagctcaag acggaaaatg cgccagaaca tcatacggcc ctcgtttgcg
    26041 tcagtgaaat gcggcgaatt ttactttcag caacgttgag cggctggtgg gtagtgagaa
    26101 tcacaatccc cccctgctct gtatgctgcg ccatacgctg ggtcagacga tcgacgccgt
    26161 taacgtcaat cgcggtaaaa ggctcgtcga ggatccataa cgtggcacgg gtcagccaca
    26221 gacgcgctaa agcgacgcgg cgttgttgcc cggcagaaag ttgatttacc ggaatatctt
    26281 cgaatccggc aagcccggcc tgcgccaggg cttccagaca ttgtgcggta tcgccatcgc
    26341 gatgataaaa gtgcagattt tctaacgccg tcagccgggt tttgatcccc ggctgatggc
    26401 ctatccataa caggttttga tggtagctgt cgcgtacctg atgcaagggc tgcccttgcc
    26461 agagaacctc gcctgcgtca gggcgagaca accccgtcag caaacggaga agcgttgtct
    26521 tccccgcgcc gttgctaccg gtgatttgta cccactctcc tgcgttcagc gtaaatgaca
    26581 agccactaaa taaggttcgt tcatcccgct cacaaagtaa ctctctgact tcaagcatac
    26641 ccacgcaata accctgttaa aaacctggct cgacttcacg catatcgggc agcttgtgcg
    26701 ctatcccttt atggcaatca atacaggttt gcccatcttt caccgcctgg tcatgcatct
    26761 tcgcggcaac cgatttctgg gcggttgtat ccatatactc gaagttgtga cagttacggc
    26821 actcctgcga gttattgtcc ttcatgcgcc gccactcatt ctgtgccatc gtcagacgat
    26881 gagcttcaaa tttctgcggc gtgtcaataa cgccaaaaat tttaccatac agctctttac
    26941 ttgctttgag cttgcgtatc atcttcggca caaactcgtg cggaacgtga caatccggac
    27001 aggtcgcacg gacgccgcta cggttgttgt agtgcacgga atccatgtat tcctgataca
    27061 ccgtgttacg catttcgtgg cagctaatgc agaactcttc ggtattggct ttttccatcc
    27121 cagtgttaaa gccgccccag aagacgatgc cgccgacaaa accgatcaac agcagcgtcc
    27181 ccagcgccag acggctgggg gtacgccacc atttccacag gcgcttaatc agaccaggct
    27241 tacggtcaga atttcccata ataacctctt atttcccgta accttttgat ggggtaaagg
    27301 tattccccac gattggcgcg gtatcggcct gcggtacgtg acattgcaga cagaaataac
    27361 gacgcggagc cacttccgcg cccactttgc cgtcgctgtc cataaagtga gtaggactga
    27421 tacgcggcgc gccagtggtg cgatagcttt cgacaccgtg gcactgcaag cagcggttgg
    27481 tattggtcgt tacctgataa ccttcaacgc tatgcgggat cattggcggc tgattcacat
    27541 agttcagcgg catccgatcc tgctcttttg gcatccgaat ggccccttcc tgcgtcccgg
    27601 atacttccgg cgactggcta aaatcgacgc cattagccgc ccaaaccgcg ccgcttacca
    27661 ccagggccag catcgccgtc cattgacaca gcgctttctt caggtcatgg cttttcatga
    27721 tttcgctccc gaactccatc gtgtagttat tgtaaataca tcctcagaac agacatccac
    27781 gcagcgaccg cacgtcatgc aatcgcggct ggtgacctgc accgggcttt gttcatccag
    27841 caccggggca cgtagcacat gcggttccgg gcaaacatga aaacaatcca tacagcggtt
    27901 acatttctgc cgatcgctgg ccgcaacggt aatcacacct ttgctaccca gcacgccata
    27961 cagcgcgcca accggacaaa tgtgcccgca ccagccgtgt tcaacgacca gtagatcaaa
    28021 taaaaacagc gcgagaataa gcagcgcgcc gctgccgaag cccattacca ggctacgccc
    28081 catcagggaa acggggttga tccattccca gatgagcgtg ccagttaagg ctgatcccac
    28141 cagaatgacc accaacagca cgtagcgaat gtggcgaggg atcgtcgcag actgattgag
    28201 gtcaaagcgc ctgcgtaacc agttcgctaa gtcggtaatc gggttcagcg ggcagaccca
    28261 gctgcaaaat aatcgctttc ctgccagggc gtagagcacg gtgataatca ccgcaccggt
    28321 caacgccacc gtggcaggca gatgaccact ggcaagactt tgcagcgtca tcagcggatc
    28381 ggtcagcgga acggtgtcga ataacaggct actgctatag ttgccgtgca agatccacac
    28441 accaaaccac ggaccgctca aaaacatccc cagcacgaag aactgacaaa ggcgacgtaa
    28501 caccagccaa cggtgactgc gccaccagcc ttttttctcc agcgcctcgc gcccggcgtc
    28561 acgtttacga tttgccattg ttcccctcca gccagccgaa gcggtaatgg tgacctaact
    28621 cccctttcgc cagtgacagc ggtaacacct tgattgccgg ttgttccagc acgcatactt
    28681 tttcgcactt accgcaaccg gtacaggcgt cgctgtgaac cgtcggcaga aagcgggcgt
    28741 gcttaccggt acgcgtgttg cgctccagct ccagggtgat ggcctcatca attttcgggc
    28801 attcgcgata acaaacatcg cagcgcagcc cctgaaagtt gagacagttt tcctggtcca
    28861 ccagtaccac cagccccatc cgcgcgtcgt cgatcgattc aatctcacga tccagcgcac
    28921 cgctcgggca cactttggcg cacggaatgt cctcacacat ttcgcaagga atatcccgtg
    28981 cgacaaaata tggcgtgccc gccgacaggc cagaggccag cgtcgccagt tttaaggtgt
    29041 cgtaagggca agcctgaaca cactgaccgc aacgcacaca ggcactggca aaggcgttct
    29101 cgtttatggc tccgggcggg cgcaaccgca cgccagatgc gcgtgcggtt tgctgttgta
    29161 accccagcgc cacacccacg gcagccagcc cgcctgctgt gcgaacgaca tcgcgcagaa
    29221 agcggcggcg accattttga ggtttcgctg accgggacat aatggcttac accttctcca
    29281 gtttgaccgc gcacttcttg aagtccgtct ctttcgagag cggatcggtc gcgtccagcg
    29341 ttagtttgtt aaccagctgt gcggcgtcga agaacggcat gtacaccagg ccctgcggcg
    29401 gacggttacg accgcgcgtt tcgacaatcg agatcacttc gccacggcga gaaaccactt
    29461 tcactttgtc gccacggcgc agatcgcgcg ctttcgcatc cagcgggtga ataaacagga
    29521 ccgcttccgg gaaggcacgg tgcagttccg gcacacggcg agtcatactg ccggtgtgcc
    29581 agtgctccag gacgcgtccg gtagagagcc acaggtcgta ctcttcatcc ggtgcttccg
    29641 ccgccggttc gaacggcagc gcgaagatca ccgctttacc atccggttta ccgtagaact
    29701 tatagccttc gcccgctttc acgtacgggt cgttaccttc gctgtaacgc cactgcgttt
    29761 ctttaccgtt caccaccggc cagcgcagac cgcgcgcttt gtggtagtca tcgaacggcg
    29821 cgagatcgtg accgtgaccg cgaccaaacc atgcgtactc ttcgaacagc cctttttgca
    29881 gatagaagcc cagctcgcgg gattcatcgt tcagctgatc ttccgccagt tcggataccg
    29941 ggaatttgct cacttcgggg gtggcataca gaacttcgta cagcgttttg ccacgcagtt
    30001 ccggtttctt cgccagcagc tcttccggcc atacttcttc agttttgaag cggcgggaga
    30061 actggactaa ctgccagagg tccgatttcg cttcgcctgg cgcctgtacc tgctgacgcc
    30121 agaactgagt acggcgttcg gcgttaccgt aagccccctc tttttctacc cacattgcgg
    30181 tcggcaggat caagtcggcg gccagcgcac tgactgtcgg atacggatcg gagacgatga
    30241 tgaagttgcg cggatcgcgc cagcccggca tacgctcttc attaatgttc ggcccggcct
    30301 gcatgttgtt ggtacacatg gtccagtaaa cattcagctt gccgtctttc agcgcacggt
    30361 cttgtgctac cgcatgcaga ccgattttcg ccggaatggt gccgctcggg atattccact
    30421 tcttctcgca gatatcacga tgtttctcgt tagttaccac catgtccgca ggcagacggt
    30481 gagcaaaggt gcccacttca cgcgcagtac cacacgcgga aggctgcccg gtcagggaga
    30541 acggaccgca acccggctgg gaaattttgc cggtcagcag gtgcaggttg tagaccaggt
    30601 tgttggccca cacgccacga gtatgctggt tgaagcccat cgtccagtag gagatgactt
    30661 tcttgttcgg atcggcatac agctgcgcca gttgttccag ctgatctttt ggtacgccag
    30721 tcatttcagc ggttttttcc agcgtatact cagcaacgaa ggctttgtaa tcttcaaagc
    30781 tcatcggttc ggaggcgtca gaacccggat tcttcgctgc tttttccagc ggatgggtcg
    30841 gacgtaaacc gtagccgatg tccgtcgccc ctttgcgcag gttaacgtgc ttgctgaaga
    30901 agtcctgatt tatcgcattg ttttgaatga tatagttggc gatgtagttc aggatcacca
    30961 ggtcagattg cggcgtaaag atgatgccgt tatccgccag ctcgaagcta cgatgctggt
    31021 aggttgaaag caccgccacg gtgacgtcct ggttagagag acgacggtta gtgatgcgtg
    31081 accagaggat cgggtgcatc tccgccatgt ttgcgcccca cagcacaaac gcgtcagcct
    31141 gctcgatgtc gtcatagcag cccatcggct catccatacc aaaggttcgc ataaagccaa
    31201 ctactgccga cgccatacag tgacgcgcat tcgggtcgat gttgttcgaa cggaagcccg
    31261 ctttaaacag cttggacgcg gcataacctt cccagatagt ccactgacca gaaccgaaca
    31321 taccgatcga ttccggccct ttttctttca gggcggtttt gaatttctct tccatcacat
    31381 cgaaggcctg atcccaggtg attggggtaa attcgccttc tttgtcatat ttaccgtttt
    31441 tcatacgcag cagcggctgc gtcaaacggt ctttaccgta catgatcttg ggcaggaaat
    31501 agcccttaat gcagttcagg ccacggttaa ccggtgcgtc cgggtcgccc tgacaggcca
    31561 ccacacgccc ctgctgcgtt ccgaccagaa cgccgcaacc agtaccgcag aaacggcacg
    31621 gcgctttatc ccatttaatg gcttcctgct gaccaacaac ggcgcgggca acgcccggca
    31681 cgctgagacc ggcagccgcc gcagcggccg caacggcgtt agctttcata aagctacgac
    31741 gactgagttt catggtgttt cctcaccttg ctcttcctgc tggtgataaa ccagcgacac
    31801 cgccagcacg ccctctacgt tgcgtactga ctcaatggtt tggatcagcg tttcgctgtc
    31861 ttctgcttcc accaccacaa tcaactgacc gctcggcgcg tcgctgacag caacttcaca
    31921 gccgggaaag gcgttcagtt gggtgctgat gtctgaaatt cgttcgcttt tggcctgcac
    31981 aaccaggctg caaacttgcc agttagtgtg catggagata ctccgcagtt atggctgata
    32041 ccggacagct ggctgcacat gctccacatc cgttgcaaag ttggctatta agttgcggct
    32101 ggtagatccc ggagagcgtc gggcgaaaga taattgccat tggctcacag ctatcctgac
    32161 agcggcggca ttcaactgac tgatacgcca ggcacgcgtc cccaatggtg aactgcaaat
    32221 cccaggccct ggtgtggcgc ggagaaaata gcgattcggg gcaagcctgc gcgcaggcgt
    32281 aacagaagct gcactcgtta tttttgaaat taacgctcgg atagccgccc gcgccgcgtt
    32341 gcagaatgtt gttttcacaa gcattaatac aggcgtcgca gcgggtgcaa tgggtcagaa
    32401 aatgagattc atcaccggac cagggcggac ggataccgtt actggctttg cgccagcgac
    32461 cagtgagtat gccccgacgg gatgcatcaa tcttcacatt gaccttccat cattaacgcg
    32521 ctctgaaaat tgagagcgac caaataaacc gcataattaa taagccattt ttatagccgc
    32581 taagatatta aaggatgtgt caaagatgca taccccgatc gaggtaaaac ctgtagcagg
    32641 atcaaaagag tggcgggaag cgtggcaaaa acgg
//
LOCUS       DBFIFM010000031        32585 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.031, whole genome shotgun sequence.
ACCESSION   DBFIFM010000031 DBFIFM010000000
VERSION     DBFIFM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.031"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(134..3283)
                     /gene="acrB"
                     /locus_tag="N7C74_001303"
     CDS             complement(134..3283)
                     /gene="acrB"
                     /locus_tag="N7C74_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HGU2431201.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSIEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHNHTVDHH"
     gene            complement(3306..4499)
                     /gene="acrA"
                     /locus_tag="N7C74_001304"
     CDS             complement(3306..4499)
                     /gene="acrA"
                     /locus_tag="N7C74_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HGU2431202.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4641..5288
                     /gene="acrR"
                     /locus_tag="N7C74_001305"
     CDS             4641..5288
                     /gene="acrR"
                     /locus_tag="N7C74_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HGU2431203.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5416..8778
                     /gene="mscK"
                     /locus_tag="N7C74_001306"
     CDS             5416..8778
                     /gene="mscK"
                     /locus_tag="N7C74_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HGU2431204.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8817..8981)
                     /gene="rsmS"
                     /locus_tag="N7C74_001307"
     CDS             complement(8817..8981)
                     /gene="rsmS"
                     /locus_tag="N7C74_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HGU2431205.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LQSDEASQSE"
     gene            complement(8995..9522)
                     /gene="priC"
                     /locus_tag="N7C74_001308"
     CDS             complement(8995..9522)
                     /gene="priC"
                     /locus_tag="N7C74_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HGU2431206.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFNRHLFQTRAT
                     TLQACLDKAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PQIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9592..9969
                     /gene="ybaN"
                     /locus_tag="N7C74_001309"
     CDS             9592..9969
                     /gene="ybaN"
                     /locus_tag="N7C74_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HGU2431207.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     CFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10122..10673
                     /gene="apt"
                     /locus_tag="N7C74_001310"
     CDS             10122..10673
                     /gene="apt"
                     /locus_tag="N7C74_001310"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HGU2431208.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGINKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10802..12733
                     /gene="dnaX"
                     /locus_tag="N7C74_001311"
     CDS             10802..12733
                     /gene="dnaX"
                     /locus_tag="N7C74_001311"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HGU2431209.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEDSIRPI"
     misc_feature    12066..12130
                     /gene="dnaX"
                     /locus_tag="N7C74_001311"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12786..13115
                     /gene="ybaB"
                     /locus_tag="N7C74_001312"
     CDS             12786..13115
                     /gene="ybaB"
                     /locus_tag="N7C74_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HGU2431210.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13115..13720
                     /gene="recR"
                     /locus_tag="N7C74_001313"
     CDS             13115..13720
                     /gene="recR"
                     /locus_tag="N7C74_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HGU2431211.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13830..15704
                     /gene="htpG"
                     /locus_tag="N7C74_001314"
     CDS             13830..15704
                     /gene="htpG"
                     /locus_tag="N7C74_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HGU2431212.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            15885..16529
                     /gene="adk"
                     /locus_tag="N7C74_001315"
     CDS             15885..16529
                     /gene="adk"
                     /locus_tag="N7C74_001315"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HGU2431213.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16661..17623
                     /gene="hemH"
                     /locus_tag="N7C74_001316"
     CDS             16661..17623
                     /gene="hemH"
                     /locus_tag="N7C74_001316"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HGU2431214.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17620..18579)
                     /gene="aes"
                     /locus_tag="N7C74_001317"
     CDS             complement(17620..18579)
                     /gene="aes"
                     /locus_tag="N7C74_001317"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HGU2431215.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATKAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQVIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18731..20035
                     /gene="gsk"
                     /locus_tag="N7C74_001318"
     CDS             18731..20035
                     /gene="gsk"
                     /locus_tag="N7C74_001318"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HGU2431216.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20168..21844)
                     /gene="ybaL"
                     /locus_tag="N7C74_001319"
     CDS             complement(20168..21844)
                     /gene="ybaL"
                     /locus_tag="N7C74_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HGU2431217.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22081..23301)
                     /gene="fsr"
                     /locus_tag="N7C74_001320"
     CDS             complement(22081..23301)
                     /gene="fsr"
                     /locus_tag="N7C74_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HGU2431218.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23519..25171
                     /gene="ushA"
                     /locus_tag="N7C74_001321"
     CDS             23519..25171
                     /gene="ushA"
                     /locus_tag="N7C74_001321"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HGU2431219.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKLGNPENFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGDHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25208..25687)
                     /gene="ybaK"
                     /locus_tag="N7C74_001322"
     CDS             complement(25208..25687)
                     /gene="ybaK"
                     /locus_tag="N7C74_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HGU2431220.1"
                     /translation="MTPAVKSLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(25891..26685)
                     /gene="ybaP"
                     /locus_tag="N7C74_001323"
     CDS             complement(25891..26685)
                     /gene="ybaP"
                     /locus_tag="N7C74_001323"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HGU2431221.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQKLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            26823..27164
                     /gene="ybaQ"
                     /locus_tag="N7C74_001324"
     CDS             26823..27164
                     /gene="ybaQ"
                     /locus_tag="N7C74_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HGU2431222.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27379..29883)
                     /gene="copA"
                     /locus_tag="N7C74_001325"
     CDS             complement(27379..29883)
                     /gene="copA"
                     /locus_tag="N7C74_001325"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HGU2431223.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADFDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30145..31077
                     /gene="glsA"
                     /locus_tag="N7C74_001326"
     CDS             30145..31077
                     /gene="glsA"
                     /locus_tag="N7C74_001326"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HGU2431224.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGELVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31080..32372
                     /gene="ybaT"
                     /locus_tag="N7C74_001327"
     CDS             31080..32372
                     /gene="ybaT"
                     /locus_tag="N7C74_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGU2431225.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
BASE COUNT         7903 a         8588 c         8551 g         7543 t
ORIGIN      
        1 ttttatgaat tttatacgtg gttaatactg gttttcgtat gagatcctga gttggtggtt
       61 caattactcc ttaatgttcg taggttatgc ataaaaaagg ccgcttacgc ggccttagtg
      121 attacacgtt gtatcaatga tgatcgacag tatggttgtg ctcgatatct tcattcttgc
      181 ggctaaagcg gcggcgaacc accacaaaga ataccggaac gaagaagatt gccagtaccg
      241 ttgcggtcac catcccgccc attacaccgg tacctactgc gttctgcgcg ccggaaccag
      301 caccagtact gataaccagc ggcataacgc cgaggataaa cgccagcgag gtcatcagga
      361 tcggacgtaa acgcatccgc accgcatcaa gcgtcgcttc aatcagacct ttaccttctt
      421 tatccatcaa gtctttggcg aattcgacga taagtatcgc gttcttcgcc gacaacccaa
      481 tggttgtgag caggcctacc tggaagtaaa cgtcattggt caggccacgg aaggtggcag
      541 ccagcaacgc accgataacc cccagcggaa cgaccagcat aacggagaac ggaatcgacc
      601 agctctcgta cagcgccgcc agacacagga acacgacaat caacgaaatc gcgtacagtg
      661 aaggtgcctg gttgccggag agacgttcct gataggacat ccccgtccag tcatagccaa
      721 caccggtagg cagtttgctc gccagttgtt ccatcagctc cattgcttca ccggtacttt
      781 tacccggtgc cgcctggcct aagatttcca tggatggcag gccgttgtaa cgttccagac
      841 gcggcgaacc gtactcccaa cgagaagagg agaacgccga gaatggcacc atctgaccat
      901 cagcagcacg aacataccag tcgccgatat catccggcag catacggtat ttcgcttctg
      961 acatgacgta aactttcttc acacgaccgc ggtcgataaa gtcgttcaca tagctgccgc
     1021 cccatgcagc gcccagagtg gtgttaatgt cgttgataga aacacccagc gcctgcgctt
     1081 tttcctggtc gatatcaatc ttaaactgcg gggtatcttc cagaccgttt ggacgtacgc
     1141 tggtcaacat atcagggtgc ttcgctgctt ctgcaagcaa ctggttacgc gcctgagtca
     1201 gtttttcgtg accaaggcca gcctggtcaa tcagctcaaa gtcaaagccg gttgcggtac
     1261 ccagttccac gattgcgggc aggttaaagg cgaaaaccat cgcatctttg atttgcgaga
     1321 aagcgcgtgt tgcacgcatg gtaatcgctt caactttgtt ttcttcaccc ggacgatcgg
     1381 cccagtcctt caaggaaacg aacgcaatac cggtattctg accacgtccc gcaaagccga
     1441 agccgttaac ggcgaacacc gactcaacgt tgttcttttc tttggtcaga tagtaatgcg
     1501 ttacctcatt gagcactttc tgcgtacgtt cctgcgttgc acctgctggc agctgaacca
     1561 tggtcataaa tacgccctgg tcctcatctg gcaagaagga gcttggcaga cgcacaaaca
     1621 gataggccat gccgaccacg atgatcagat acagcaccag ataacgcccc gtactgcgca
     1681 gaataccgcc tacgctgtcg gtgtagtggt gcgtgctctt ctcgaacatg cggttaaacc
     1741 agccgaagaa gcctttttta ccttccccgt gatcgccttt ggcaatcggt ttcagcatgg
     1801 tggcacaaag agctggagtc aggatcaacg ccaccagtac cgacagcgcc attgctgaaa
     1861 caatggtaat agagaactga cgataaattg ccccagtaga accgccaaag aaggccatcg
     1921 gtacgaatac cgccgacagt accatcgcga taccgaccag agcgccctga atctgcccca
     1981 tcgacttacg ggtggcttct tttggcggca aaccttcttc cgccatcaca cgctcaacgt
     2041 tttctaccac aacgatagcg tcatccacca acaggccgat ggcgagcacc atcccgaaca
     2101 ttgttagcgt gtttatcgag aagccaaagg cggcaaggac ggcaaaggtc ccgagcaata
     2161 ccaccggtac ggcaatggtc ggaatcaacg tcgcgcggaa gttctgcagg aacagataca
     2221 taaccaggaa cacgaggatg atcgcttcga ccagcgtttt aaccacttcg tgaatagaga
     2281 ttttcacgaa cggcgtggtg tcgtacgggt aaacaatttt cagacccgac gggaagaacg
     2341 gttccatctt cgccagttca gcacggattg ccgcagcggt atccagcgcg tttgcaccgg
     2401 tcgccagctt gatccccaga ccggaagccg gttggccgtt aaactctgcg atgatgtcgt
     2461 agttctcacc acccagctca atcttcgcca catcacgcag cagtacgcgg gaaccatcct
     2521 gattcacttt cagcaggatt ttgccgaact cttcagtaga ggtcagacgc gtctgagcaa
     2581 taatagaggc gttaagctgt tggcctttca ccggcggtgt accaccgagc tggcccgccg
     2641 caacctgggc gttctgcgct ttgatggcgg taataacatc aaccggcgtt agctggaatt
     2701 tgttcagttc attcgggttc atccagatac gcatcgcgta ctgtgaaccg aacaactgaa
     2761 cgtcacccac gccagacgta cggctgatgg catctttcat attcgccgcc acgtagtcgg
     2821 agatatcctc ctgcgtcatg gtgccatcgg tgttgataac gccgacaacc atcaggaagc
     2881 tgctggatga tttctcaatg ctcacccctt gctgctgaac ttcttgcggc agcaacggca
     2941 tcgccagctg cagtttgttc tgcacctgaa cctgcgcgat atccgcatca gtaccagact
     3001 caaaggtcag ggtgatctgt acggtacccg tggagtcact gttagaggac atgtacatca
     3061 ggttatcgat accgttcata ttctgttcga taacctgtgt caccgtgtcc tgcactgttt
     3121 tcgcatcagc gccggggtag gaggcggaga tcgttactgc cggcggtgca atcgtaggat
     3181 attgcgccac cggcagtttg aggatcgcca gcccccctgc caacatgatg ataatggcga
     3241 tcacccacgc aaaaatcggg cgatcgataa agaaattagg catgtcttaa cggctcctgt
     3301 ttaagttaag acttggactg ttcaggctga gcaccgcttg cggcttgctg gttattatca
     3361 gcggtaactt cttgtgcttt tacctggaca ccaggacgca ctttctgcag cccacttatt
     3421 actacgcgat cgcctgcttt cagaccttct gtcaccagcc acttatcgcc aatagcctgg
     3481 cttgcaacga tcggacgggt ttccactttg tcatccgcgc caaccaccag tacggtggca
     3541 tcgccacgcg gcgtacgggt tacgccctgt tgcgggacta aaatagcgtt tggattaagc
     3601 ccttcttcca gacgtgcgcg cacgaacata cccggcagca gagtgtgatc cgggttcggg
     3661 aagatagcgc gtagggtgat agacccagtg gtctgatcaa cggtaacgtc agagaattcc
     3721 agcgtaccgt cctgcgggaa cttaatgccg tcactggtga tcagcgacac tttggctttg
     3781 ccgttctctt gtttcagcgt gccattcgcc agttcctgtt tcaggcgcag gaagtcgttg
     3841 ctggactggg tcacatcaac gtagatcgga tcaagttgct gcacggttgc cagcgcagtc
     3901 gcctgaccgt tctgtaccaa tgcgccttcc gtcacgttcg acttaccaat gcgaccgcta
     3961 atcggagagg tgactttggt gtaagccaga ttgatccgcg cagtttcaac ggcagctttc
     4021 gccgcagtta ccgcagcatt cgcctgttgc gcatcagcca gagcctgatc gtactcttgc
     4081 ttactgatgt actgagtacc gagcagtttc tgataacgat tcaccgtcaa ttgcgcgata
     4141 ttggctgcag cctgggcttt cgccagatca cctttcgcac tgtcgtatgt cgcctgatag
     4201 gtcgcaggat caatctgata gagagagaca cctgcttcga tgtcgctacc ttctttgaaa
     4261 ttacgcttca ggataatccc gctaacttga ggacgaactt ctgcgatccg gtaggcactg
     4321 gtgcgacccg gaagctcggt tgtgatctgc agaggttcag ttttgactgt tactacgcca
     4381 acggcgggca tctgctggcc accttgttgg gcctgtttgt cgtcacatcc tgttagggct
     4441 aagctgcctg agagcatcag aacgaccgcc agaggcgtaa accctctgtt tttgttcata
     4501 tgtaaacctc gagtgtccga tttcaaattg gtcaatggtc aaaagttaat aaacccattg
     4561 ctgcgtttat attatcgtcg tgctatggta catacattca caaatgtatg taaatctaac
     4621 gcctgtaaat tcacgaacat atggcacgaa aaaccaaaca agaagcgcaa gaaacgcgcc
     4681 aacacatcct cgatgtggct ctacgtcttt tctcacagca gggggtatca tccacctcgc
     4741 tgggtgagat tgcaaaagca gctggcgtta cgcgcggtgc aatctactgg cattttaaag
     4801 acaagtcgga tttgttcagt gagatctggg aactatcaga atccaatatt ggtgaactag
     4861 agcttgagta tcaggcaaaa ttccctggcg atccactctc agtattaaga gagatattaa
     4921 ttcatgttct tgaatccacg gtgacagaag aacggcgtcg attattgatg gagattatat
     4981 tccacaaatg cgaatttgtc ggagaaatgg ctgttgtgca acaggcacaa cgtaatctct
     5041 gtttggaaag ttatgaccgt atagaacaaa cgttaaaaca ttgtattgaa gcgaaaatgt
     5101 tgcctgcgga tttaatgacg cgtcgggcag caattattat gcgcggctat atttccggcc
     5161 tgatggaaaa ctggctcttt gccccgcaat cttttgatct taaaaaagaa gcccgcgatt
     5221 acgtcgccat cttactggag atgtatctcc tgtgccccac gcttcgtaat cctgccacta
     5281 acgaataacc ctgaatctga ctccaggatt tttcctggac attttcgtcg ttgctattct
     5341 ggttcactgc gtcgtgatat tcttgcggtt tgactttttc aggtcgttct tcaggttcag
     5401 aaaccttcat tcatcatgac tatgttccag tattacaaac gatcacggca ttttgttttt
     5461 tcagcattta ttgcttttgt ttttgtcttg ttatgccaga acacggcgtt tgcgcgggcg
     5521 tcatcgaatg gtgatctgcc gacaaaagcg gacctgcagg cgcaacttga ctcactaaat
     5581 aaacaaaaag atctttctgc tcaggacaaa ctggtgcagc aggatctgac agatacatta
     5641 gccaccctcg ataaaatcga tcgcgtaaaa gaagagaccg ttcagctacg gcaaaaagtc
     5701 gctgaagcgc cggaaaaaat gcgccaggcg accgcggcgt taacagcact tagcgatgtc
     5761 gataacgacg aagaaacgcg caaaattctg agcacgcttt cgttgcgcca gctggaaact
     5821 cgcgttgccc aggcgctgga cgatttgcaa aacgcacaaa acgatctggc gtcttataac
     5881 agccagctgg tttcgttaca gacgcagccc gaacgcgtgc aaaatgcgat gtataacgct
     5941 tcgcagcagc tgcaacaaat tcgcagtcgt ctggatggga ctgatgtcgg cgagacggcc
     6001 ttacgtccca gccagaaagt gttaatgcag gctcagcagg cgttgctgaa tgcggagatt
     6061 gaccagcagc gtaaaagcct ggaagggaat accgtcttgc aggatacctt gcaaaagcaa
     6121 cgtgattacg tgacggcgaa cagcgcccgt ctggagcacc agttgcagct gttgcaagaa
     6181 gcggtaaaca gcaagcgcct gactttaacc gaaaaaacgg cgcaggaagc cgtctccccg
     6241 gatgaagccg cgcgtattca ggctaatccg ctggtgaagc aggaactgga aattaaccag
     6301 cagttaagtc agcgtctgat taccgcgact gaaaacggta atcagttgat gcagcaaaac
     6361 attaaagtca aaaactggct ggagcgggcg ctgcaatcgg aacgcaatat taaagagcag
     6421 attgccgtcc tgaagggcag cctgctgttg tctcgtatcc tttaccagca acaacaaacg
     6481 ctgccctcgg cggatgaact ggaaaacatg accaaccgca tcgcggattt gcgtctcgaa
     6541 cagtttgaag ttaaccagca gcgtgatgca ctcttccaga gcgatgcgtt cgtcaacaaa
     6601 ctggaagaag gtcacaccaa cgaagtcaac agcgaagttc acgatgcgtt attgcaagtg
     6661 gttgatatgc gtcgcgaatt gctggatcaa ctcaacaaac agttgggtaa ccagctgatg
     6721 atggccatta acctgcaaat caaccagcag cagttaatga gtgtgtcgaa aaacctgaaa
     6781 tccatcctga ctcagcaaat cttttgggtg aacagtaacc gtccaatgga ctgggactgg
     6841 atcaaagcgt tcccgcaaag cctgaaagat gaatttaagt cgatgaaaat tacggttaac
     6901 tgggaaaaag cctggcccgc cgtttttatc gctttcctcg ctggtttgcc gctgctgttg
     6961 attgccgggc tgatccactg gcgtctgggg tggctgaaag cgtatcaaca aaaactggct
     7021 tccgctgtgg gttccctgcg taacgacagc cagctcaaca caccaaaagc gatccttatc
     7081 gacctgatcc gtgcactgcc ggtgtgcctg attattctcg cggttggcct gattctgttg
     7141 accatgcagc tcaacatcag cgaactgcta tggtcgttca gcaaaaaact ggcgattttc
     7201 tggctggtgt ttggcctgtg ctggaaagtg ctggagaaga acggcgttgc cgtgcgtcac
     7261 ttcggcatgc cggaacagca gaccagccac tggcgtcggc aaattgtccg catcagtctc
     7321 gcgttgctgc ctatccattt ctggtctgtg gtggcagaac tttccccgct gcatctgatg
     7381 gatgatgtgc tggggcaggc gatgattttc ttcaacctgc tgctgattgc cttcctggta
     7441 tggccaatgt gccgcgaaag ctggcgtgat aaagagtcgc acaccatgcg actggtcacc
     7501 attaccgtgc tgtcgattat cccgattgca ttgatggtgc tgactgccac gggttatttc
     7561 tacaccacgc tgcgcctctc cgggcgttgg attgaaaccg tttatctggt gatcatctgg
     7621 aacttgctct accagacggt attgcgcggc ttaagcgtag cggcgcggcg tatcgcctgg
     7681 cgtcgtgcgc tggcgcgtcg gcagaatctg gtgaaagagg gcgcagaagg tgccgaaccg
     7741 ccggaagaac ccaccattgc actggaacag gttaaccagc agacgctgcg tattaccatg
     7801 ttgctgatgt ttgcgctgtt cggtgtcatg ttctgggcaa tttggtccga tttgatcacc
     7861 gtgttcagct atctcgacag cattacgctg tggcattaca acggtactga agctggtgct
     7921 gcggtggtga aaaacgtcac catgggtagt ctgttgtttg cgattatcgc ctcaatggtg
     7981 gcctgggcgt tgattcgcaa cctgcctggt ttgctggaag tgctggtgct ctcgcgactg
     8041 aatatgcgcc agggcgcgtc gtatgccatt actaccatcc ttaactacat cattattgct
     8101 gttggtgcga tgacggtgtt cggatcgctg ggcgtctctt gggataaact ccagtggctg
     8161 gccgcagcat tatccgtagg tcttggtttt ggtttacaag aaattttcgg taacttcgtc
     8221 tccggtttga tcattctgtt cgaacgtccg gtgcgtattg gcgatacggt aaccattggt
     8281 agcttctcgg ggacggtaag taaaatccgt attcgtgcga caacgattac cgatttcgat
     8341 cgcaaagaag tgatcatccc gaacaaagcg tttgttaccg agcgtctgat caactggtcg
     8401 ttgactgaca ctactacgcg tctggtgatc cgtctcggcg tggcctatgg ctccgatctg
     8461 gaaaaagtgc gtaaagtgtt actgaaggcg gcgactgagc acccaagggt gatgcacgaa
     8521 ccaatgccgg aagtcttctt tacggcattt ggtgccagca cgttggatca tgagctgcgt
     8581 ctgtatgtgc gtgaactgcg tgaccgtagt cgtactgtcg atgagctgaa ccgtactatc
     8641 gatcagctgt gccgtgaaaa cgacatcaac attgccttta accagcttga agtgcatctg
     8701 cacaacgaga agggcgatga ggtgacggaa gtaaaacgcg actacaaagg cgacgatccg
     8761 acgccagcgg tagggtaagg acaataaagg gcgacagtga tgccgccctt tattttttac
     8821 tcgctttgtg aagcctcatc gctctgtaac ttcttttcat aatcacgctt tacaatatcc
     8881 agcgcgcgca gcacggtgcg ggctgggatc tgattttctt ccagcagcac aatcaaatcg
     8941 acagccagtt tgacatcgtc aggggcattt tccagtgaca tactctctcc attgctagcg
     9001 ggttaaacgc gctaacctgt tttcgatttt ttccagcgca tggcggcagc gtgccaggcg
     9061 cgcttcatag gcttccactt cacgatgcag cgtttgctgt tccacgagat cggtcacccg
     9121 cgccaggcgg gctctacgtt cggcaaccat ctcacgtagc cgccgctcaa aatcctgatg
     9181 ctgaatacgt ttacgctgcc agcgggcaat ttggggtggt gcgctatccc actcgcgtag
     9241 cgaccaggct gtggcttcac gcgctatagc ctccagttgc gccgccagat gttctgccag
     9301 ccaggcgact tgcggcagtt gctgttgctc aactgcatga cgaagcgcag ccagattatc
     9361 gcccgccttg tcgagacaag cctgtagtgt tgtcgcacga gtctgaaaaa gatgcctgtt
     9421 gaaacgagca cttagcgtgg cgaactgtga caccggggca caacgctgac gcagcgtagc
     9481 gagctgtcct tccagttttt ccagcagcag ggcggttttc acgataagac ctcaatgaaa
     9541 atgataattg ttatgctaaa gtagccactc tgtaagctga caacactcaa tatgcaacga
     9601 atcattttaa tcatcattgg ctggctggcg gtagtgctgg gcacgctggg cgtggtatta
     9661 ccggtattac cgacgacgcc gtttatcctg ctggcggcct ggtgctttgc ccgttcttcc
     9721 ccgtgctttc acgcctggtt gctgtaccgc tcatggtttg gcagctatct acgtttctgg
     9781 cagaaacatc atgcgatgcc gcgcggcgtc aaaccgcggg cgattttgct tattttgctc
     9841 acgtttgcca tttctctgtg gttcgtccag atgccatggg tgcgcatcat gttgctggta
     9901 attctcgcct gtttgctttt ctatatgtgg cgaattccgg tgattgatga aaagcaagaa
     9961 aagcactgaa gcacaacaat cgcagttgca attattgcgt acagccagta cattctggcg
    10021 ttttcgagca caggcgcagg cggtcaaagg ttaaacaact gttacttttg atacgtttaa
    10081 aacgcgccgt gagtaccacc gtaacaagca ggcatacact tatgaccgcg actgcacagc
    10141 agcttgagta tctcaaaaat agcatcaaaa gcattcagga ctacccaaaa cccggcattc
    10201 ttttccgcga tgtcaccagc ttactggaag acccgaaagc ttacgctctc agcatcgact
    10261 tgctggttga gcgttacaaa aatgcgggca ttaacaaagt tgtcggcacc gaagcgcgtg
    10321 gcttcttgtt tggcgctccg gtagctctgg gtctgggcgt tggctttgta ccggtccgta
    10381 aaccgggcaa actgccgcgt gaaaccatca gtgaaactta cgatctggaa tacggcaccg
    10441 atcagctgga gatccacgtt gatgccatca aaccgggcga caaagttctg gtggtggacg
    10501 atctgctggc aaccggcggc actatcgaag cgaccgttaa actgatccgt cgtctgggtg
    10561 gtgaagtggc tgacgctgcg ttcattatca acctgttcga tctcggcggc gaacagcgtc
    10621 tcgaaaaaca gggcattacc agctacagcc ttgtcccgtt cccgggccat taattatcgc
    10681 cagtcttgtg ctgcccacgc tacggacagc acaagatgtg cattcagcct cgccgttctg
    10741 acggggctgt gttagcatta ccccttcgag aatccacctt ccagcgtttc agagcctgcc
    10801 aatgagttat caggtcttag cccgaaaatg gcgcccacaa acctttgctg acgtcgtcgg
    10861 ccaggaacat gtgctgaccg cactggcgaa cggcttgtcg ttagggcgta ttcatcatgc
    10921 ttatcttttt tccggcaccc gtggcgtcgg aaaaacctct atcgcccgac tgctggcgaa
    10981 ggggctaaac tgcgaaaccg gcattaccgc gacgccgtgc ggcgtgtgcg ataactgtcg
    11041 tgaaatcgag caggggcgct ttgtcgatct gattgaaatc gacgccgcct cgcgcaccaa
    11101 agttgaagat acccgcgacc tgctggataa cgtccagtac gctccggcgc gtggtcgttt
    11161 caaagtttat ctcatcgacg aagtgcatat gctgtcgcgc cacagcttta acgcactgtt
    11221 aaaaaccctt gaagagccgc cggagcacgt taagtttctg ctggcgacga ccgatccaca
    11281 gaaattgccg gtgacgattt tgtcgcgctg tttgcaattt catctcaagg cgctggatgt
    11341 cgagcaaatt cgtcatcaac ttgagcacat cctcaatgaa gaacatatcg ctcacgagcc
    11401 gcgggcgctg caattgctgg cgcgcgccgc tgaaggcagc ctgcgagatg ccttaagtct
    11461 gaccgaccag gcgattgcca gcggtgacgg ccaggtttca acccaggcgg tcagtgcgat
    11521 gctgggtacg cttgacgacg atcaggcgct gtcgctggtt gaagcgatgg tcgaggccaa
    11581 cggcgagcgc gtaatggcgc tgattaatga agccgctgcc cgtggtatcg agtgggaagc
    11641 gttgctggtg gaaatgctcg gcctgttgca tcgtattgcg atggtacaac tttcgcctgc
    11701 tgcacttggc aacgacatgg ccgccatcga gctgcggatg cgtgaactgg cgcgcaccat
    11761 accgccgacg gatattcagc tttactatca gacgctgttg attggtcgca aagaattacc
    11821 gtatgcgccg gaccgccgca tgggcgttga gatgacgctg ctgcgcgcgc tggcgttcca
    11881 tccgcgtatg ccgctgcctg agccagaagt gccacgccag tcctttgcac ccgtcgcgcc
    11941 aacggcagta atgacgccaa cccaggtgcc gccgcaaccg caatcagcgc cgcagcaggc
    12001 accgactgta ccgctcccgg aaaccaccag ccaggtgctg gcggcgcgcc agcagttgca
    12061 gcgcgtgcag ggagcaacca aagcaaaaaa gagtgaaccg gcagccgcta cccgcgcgcg
    12121 gccggtgaat aacgctgcgc tggaaagact ggcttcggtc accgatcgcg ttcaggcgcg
    12181 tccggtgcca tcggcgctgg aaaaagcgcc agctaaaaaa gaagcgtatc gctggaaggc
    12241 gaccactcca gtgatgcagc aaaaagaagt ggtcgccaca ccgaaggcgc tgaaaaaagc
    12301 gctggaacat gaaaaaacgc cggaactggc ggcgaagctg gcggcagaag ccattgagcg
    12361 cgacgcgtgg gcggctcagg tgagtcaact ttcgctacca aaactggtcg aacaggtggc
    12421 gttaaatgcc tggaaagagg agagcgacaa cgcagtatgt ctgcatttgc gctcctctca
    12481 gcggcatttg aacaaccgcg gtgcacagca aaaactggct gaagcgttga gcatgttaaa
    12541 aggttcaacg gttgaactga ctatagttga agatgataat cccgcggtgc gtacgccgct
    12601 ggagtggcgt caggcgatat acgaagaaaa acttgcgcag gcgcgcgagt ccattattgc
    12661 ggataataat attcagaccc tgcgtcggtt cttcgatgcg gagctggatg aagacagtat
    12721 ccgccccatt tgatcgtaag cacagcttac gttcgtcatc cttaacgtga ttgagagaga
    12781 aacctatgtt tggtaaaggc ggtctgggta acctgatgaa gcaagcccag cagatgcaag
    12841 aaaaaatgca gaaaatgcag gaagagatcg cgcagctgga agttaccggc gaatctggcg
    12901 caggtctggt aaaagtgacc atcaacggtg cacataactg ccgtcgcgta gagatcgacc
    12961 cgagcctgct ggaagacgac aaagagatgc tggaagacct ggtggctgca gcattcaacg
    13021 acgcagcacg tcgtattgaa gaaacgcaga aagaaaaaat ggcttctgta tcctccggaa
    13081 tgcagctgcc gcctggcttt aagatgccgt tctgatgcaa accagcccgc tgttaacaca
    13141 gcttatggaa gcactgcgct gtctgccggg cgttggcccg aagtcggcgc agcgtatggc
    13201 gttcacactg cttcagcgcg atcgtagcgg cgggatgcgt ctggcgcagg cgctcacccg
    13261 ggcgatgtcg gaaatcggcc actgcgccga ttgccgcacc ttcaccgaac aggaagtctg
    13321 taacatctgt tcgaatccgc gtcgtcagga aaacggtcaa atctgcgtgg tggagagtcc
    13381 ggcggacatt tacgccattg agcagacggg gcagttttca ggtcgttatt ttgtgttgat
    13441 gggacatctg tcaccgctgg acggcatcgg tccggatgat atcggccttg atcgtctgga
    13501 acagcgtctg gcagaggaaa aaatcactga agtgatcctc gccaccaacc ctacggttga
    13561 aggtgaagct accgctaact acattgccga gctttgcgcg caatatgacg tggaagccag
    13621 ccgaatcgct catggcgtac cggttggcgg cgaactggaa atggtcgatg gcaccacgct
    13681 gtcacactcc cttgccgggc gtcataagat tcgtttttaa gcaaacgaga gcaggatcac
    13741 ctgctctcgc ttgaaattat tctcccttgt ccccatctct ccctcatcct gtttttaacc
    13801 ttaaaatggc attattgagg tagacctaca tgaaaggaca agaaactcgt ggttttcagt
    13861 cagaagtgaa acagcttctg cacctgatga tccattctct ctattccaat aaagaaatct
    13921 tcctgcgtga gcttatctct aacgcctccg atgcggcgga caagttgcgt ttccgtgcgc
    13981 tctctaaccc ggacctgtac gaaggtgatg gcgaactgcg cgttcgtgtc tctttcgata
    14041 aagacaagcg cacgctgacc atctccgata acggcgtggg gatgacccgc gacgaagtga
    14101 ttgaccatct ggggactatc gctaaatccg gtaccaaatc attcctcgaa tccctgggtt
    14161 ctgaccaggc gaaagacagc cagctgatcg gtcagtttgg tgttggtttc tactctgcgt
    14221 ttatcgtggc cgacaaagtg accgtgcgta ctcgcgcggc aggcgaaaaa ccagaaaacg
    14281 gcgtcttctg ggaatcggct ggcgaaggtg aatataccgt tgccgacatc accaaagaag
    14341 atcgtggtac tgaaatcacc ctgcatctgc gtgaaggcga agacgagttc ctcgatgact
    14401 ggcgcgtgcg ttccatcatc agcaaatact ccgaccatat cgcgctgccg gtagagatcg
    14461 aaaaacgcga agagaaagac ggcgaaaccg ttatctcctg ggagaaaatt aacaaagcgc
    14521 aggcgctgtg gactcgtaac aagtcggaaa tcaccgatga agagtacaaa gagttctaca
    14581 aacacatcgc ccacgatttt aacgatccgc tgacctggag ccacaaccgt gttgaaggta
    14641 agcaggagta caccagcctg ctgtacattc cgtcccaggc tccgtgggat atgtggaacc
    14701 gcgatcataa acacggcctg aaactgtatg ttcagcgtgt gttcatcatg gacgacgcag
    14761 aacagttcat gccgaactat ctgcgcttcg tgcgtggtct gattgactcc agcgatctgc
    14821 cgctgaacgt ttcccgtgaa atcctccagg acagcacggt aacgcgtaac ctgcgcaatg
    14881 cgctgaccaa gcgtgtgctg caaatgctgg aaaaactggc gaaagacgac gcggaaaaat
    14941 accagacctt ctggcaacag tttggcctgg tactgaaaga aggtccggcg gaagatttcg
    15001 ctaaccagga agcgatcgcc aaattgctgc gttttgcttc tacccatacc gattcttctg
    15061 cgcagaccgt gtctctggaa gactacgttt cccgcatgaa agaagggcag gagaaaatct
    15121 actacatcac tgcagacagc tatgcggcag cgaagagcag cccgcacctg gaactgctgc
    15181 gtaagaaagg catcgaagtt ctgctgcttt ccgaccgcat cgatgagtgg atgatgaact
    15241 atctgactga gttcgacggt aaaccgttcc agtcggtgtc taaagttgac gagtcgctgg
    15301 aaaaactggc tgacgaagtt gatgagagcg cgaaagaagc ggagaaagca ctgactccgt
    15361 tcatcgaccg tgtgaaagcc ctgctcggcg agcgcgtgaa agatgtccgt ctgactcacc
    15421 gtctgaccga tacgccagcg atcgtttcga ccgacgcgga cgaaatgagc actcagatgg
    15481 cgaaactgtt cgctgcggcg ggccagaaag tgccagaagt gaaatacatc ttcgaactga
    15541 acccggatca cgtactggtg aaacgtgcgg cagatactga agatgaagcg aagttcagcg
    15601 agtgggtaga actgctgctg gatcaggcac tgctggcaga acgcggcacg ctggaagatc
    15661 cgaacctgtt tattcgtcgt atgaaccagc tgctggtttc ctgatgtaat gccggatgac
    15721 cttcgtgtca tccggcattt ttcttttcat tatctgcact ttccgcaaat tatctcgcca
    15781 ttaaccgttt cagcccctgg tgcctttctt gaggcaatcg cctgatggtg gtatcgttta
    15841 tcgctttttc aaaaaattcg acacatttta aggggatttt cgcaatgcgt atcattctgc
    15901 ttggcgctcc gggcgcgggg aaagggactc aggctcagtt catcatggag aaatatggta
    15961 ttccgcaaat ctccactggc gatatgctgc gtgctgcggt caaatctggc tccgagctgg
    16021 gtaaacaagc aaaagacatt atggatgctg gcaaactggt caccgacgaa ctggtgatcg
    16081 cgctggttaa agagcgcatt gctcaggaag actgccgtaa tggtttcctg ttggacggct
    16141 tcccgcgtac cattccgcag gcagacgcga tgaaagaagc gggcatcaat gttgattacg
    16201 ttctggaatt cgacgtaccg gacgaactga tcgttgaccg tatcgtcggt cgccgcgttc
    16261 acgcgccgtc tggtcgtgtt tatcacgtta aattcaatcc gccgaaagta gaaggtaaag
    16321 acgacgttac cggtgaagaa ctgactaccc gtaaagacga tcaggaagaa accgtacgta
    16381 aacgtctggt tgaataccat cagatgacag caccgctgat cggctactac tccaaagaag
    16441 cggaagcggg taacaccaaa tacgcgaaag ttgacggcac caagccggtt gctgaagttc
    16501 gcgctgatct ggaaaaaatc ctcggctaat caagccgcag cagatctgct ttcgggcagg
    16561 tctgctcatc cacctcaggc aatacctctc acagcaatta gttcttcttc ctcacttttc
    16621 cgctacaatt atcaacaagt tgaatcgata agaggcggta atgcgtcaga ctaaaaccgg
    16681 tatcctgctg gcaaacctgg gtacgcccga tgcccccaca cctgaagcgg taaaacgcta
    16741 tctgaaacaa tttttaagcg acagacgcgt ggttgatacc tcacggttgt tatggtggcc
    16801 attgctgcgc ggcgtgattt tgccgctgcg ctcgccgcgt gtggcgaagc tgtatgcctc
    16861 tgtctggatg gaaggtggct cgccgctgat ggtttacagc cgccagcaac agcaggcgct
    16921 ggcacaacgt ttaccggaga cgcccgtagc gctgggaatg agctacggct cgccatcact
    16981 ggaaagcgcc gtagatgaac tcctggcaga gcatgtagat catattgtgg tgctgccgct
    17041 ttatccgcaa ttctcctgtt ctacggtcgg tgcggtatgg gatgaactgg cacgcattct
    17101 ggcgcgcaaa cgtagcattc cggggatatc gtttatacgt gattacgccg ataaccacga
    17161 ttacattaat gcactggcga acagcgtacg cgcttctttt gccaaacatg gcgaaccgga
    17221 tctgctgctg ctctcttatc atggcattcc ccagcgttat gcagatgaag gcgatgatta
    17281 cccgcaacgt tgccgcacaa cgactcgtga actggcttcc gcattaggga tggcaccgga
    17341 aaaagtgatg atgacctttc agtcgcgctt tggtcgggaa ccctggctga tgccttatac
    17401 cgacgaaacg ctgaaaatgc tcggagaaaa aggcgtaggt catattcagg tgatgtgccc
    17461 gggctttgct gcggattgtc tggagacgct ggaagagatt gccgagcaaa accgtgaggt
    17521 cttcctcggt gccggcggga aaaaatatga atatattccg gcgcttaatg ccacaccgga
    17581 acatatcgaa atgatggcta atcttgttgc cgcgtatcgc taaagctgag cggtaaagaa
    17641 ctgagcgccg tcgcgaagag cctcgtcggc ggttttcatc atccgtgaat aatgcaaaaa
    17701 ggcgtgcagc gtgcctgggt agagtttgaa ctcacagggc tgctgatgcg ccgctaacgt
    17761 ctggtaaagc agacggctgt catccagcag cggatcgaac tccgccccgg caataaaaca
    17821 gggcggaact tcgcgagtga gatcattatt aaacagacag taatacggcg actcgcggtc
    17881 cgcgtcgttg cttaaatacg cctcttcata catctgcaaa tcctgttgcg ttaagccatc
    17941 ccagacaccg cccaacagac gacgagtcac ggaatcccgt aatccgtaaa gcccatacca
    18001 cagcaaaacg cccgcaactt taccgcaatc gatctgttta tcacgcaacc acaacgcact
    18061 ggcgagcgcc agcatggcac ctgcggaatc accggcaaag ccaatgcggg acatattgat
    18121 ttgataatcc tccgcctgct ggtggaaata acaacaagca gccacaattt cctctatcac
    18181 ttgcggaaaa cgcgcttcag gtgaaagggt gtaatcaata ccaatcaccg tacattggct
    18241 gtagcttgcc agcaggcgca tgatgcgatc gtgggtatcg agattgccga gaataaaacc
    18301 gcctccatgc aaataaaata gcgttgccgg gctatctggc tgcggacaaa agagacgtgt
    18361 ttccacctgc ccatattttg ttggaaccat gtaagctttg gttgccattt ctggagcgcc
    18421 cgcattccag aatcggcgct caagcgtgta atactgtcgt tgctcagcaa tcgttcccgt
    18481 tgcgggccag ggcggtaaat ccggctgaag agtattcaca acggtcttca tttcagcaga
    18541 aataaggtcc agaacaggta atttgttttc cggcttcata caaaaactcc tttcaaatta
    18601 cgtcattgta aggaaccact gccatgaaaa tgcgatcccg cctgctgata ttgaaactgg
    18661 ctgcgtctcg cgcgctcccg tcagattgtg ttaacattcg ccgctcagtt aaccacccgt
    18721 aaaaacaacc atgaaatttc ccggtaaacg taaatccaaa cattacttcc ccgtaaatgc
    18781 acgcgatccg ctgcttcagc aattccagcc agaaaacgaa accagcgccg cctgggtagt
    18841 gggtatcgat caaacgctgg tcgatattga agcgaaagtg gatgatgaat tcattgagcg
    18901 ttatggatta agcgccgggc attcactggt gattgaggat gatgtagccg aagcgcttta
    18961 tcaggaacta aaacagaaaa acctgattac ccatcagttt gcgggtggca ccattggtaa
    19021 caccatgcat aactactcgg tgctcgcgga cgaccgttcg gtgctgctgg gcgtcatgtg
    19081 cagcaatatt gaaattggca gctatgccta tcgttacctg tgtaacacct ccagccgtac
    19141 cgatcttaac tatctacaag gcgtggatgg cccgattggt cgttgcttta cgctgattgg
    19201 cgagtccggg gaacgtacct ttgctatcag ccctggccac atgaaccagc tgcgggctga
    19261 aagtattccg gaagatgtga ttgccggagc ctcggctctg gttctcacct cttatctggt
    19321 gcgttgcaag ccgggtgaac ccatgccgga agcaaccatg aaagccattg agtacgcgaa
    19381 gaaatataac gtaccggtgg tgctgacgct gggaactaag tttgtcattg ccgagaatcc
    19441 acagtggtgg cagcaattcc tcaaagacca cgtctctatc cttgcgatga acgaagatga
    19501 agccgaagcg ttgaccggag aaagcgatcc gttgttggca tctgacaagg cgctggactg
    19561 ggtagatctg gtgctgtgca ccgccgggcc aatcggcttg tatatggcgg gctttaccga
    19621 agacgaagcg aaacgtaaaa cccagcatcc gttgctgccg ggcgctatag ccgaattcaa
    19681 ccagtatgag tttagccgcg ccatgcgcca caaggattgt cagaatccgc tgcgtgtcta
    19741 ttcgcacatt gcgccgtaca tgggcgggcc ggaaaaaatc atgaacacca acggagcagg
    19801 ggatggcgca ctggcagcgt tgctgcatga cattaccgcc aacagctacc atcgtagcaa
    19861 cgtaccaaac tccagcaaac ataaattcac ctggttaact tattcatcgt tagcgcaggt
    19921 gtgtaaatat gctaaccgtg tgagctatca ggtactgaac cagcattcac ctcgtttaac
    19981 gcgcggcttg ccggagcgtg aagacagcct ggaagagtct tactgggatc gttaagttat
    20041 cgtcggttcg taggccagat aaggcgttca cgccgcatct ggcattttgc tctcaatgcc
    20101 tgatgcgacg ctggcgcgtc ttatcatgcc tacatatttt tcatatttta catccggcaa
    20161 ccaccgttta ccccgtcacc acttcacccg ccggtggcgt ttccagcagt tccagcatag
    20221 tacgggcaat ttcacgctcg cccatcacta cttgattcgc accacgttcg gtgatatacg
    20281 ccacttcatc gtcataatgg gcgcgggcaa taatctcaat atccggattt ttcgcgcggg
    20341 cagatgccac aatctcaccc gcttcataac cgttggggat cgtcaggatc agccattttg
    20401 cacattccag atgcgccagt tgcataattt cttcgttcgc cgcattgccc aatactgcgc
    20461 ggaccccgcg ctctcgcagc tcatcaacac gggttcgtga cgtctcaatc accaccagcg
    20521 gaatatcaga ggcgagcaat ttctccccca gcaggctgcc tacacgaccg taacccacca
    20581 atagcgcatg gttgcaaata tccactggga tctgcttctc ttcttcgatt gcctcttcca
    20641 gcgtctgctc ttccagcgtt tcggtcttcg ccagatattt ctccagtagt gcgaacagta
    20701 ctgggttgag cataatcgac aggatcgccc ctgccagtac caggttttgt ccggcctgcg
    20761 gcagtaaatt caatgccatt cccagtcccg ccaggataaa cgcgaactca ccaatctgcg
    20821 ccaggctggc ggcgatagtt aatgccgtac gttgggagtg accaaacagt cgcaccagga
    20881 aaaacgcggc taacgactta ccaaacagaa taatcgccag cgtcgccagc actgccagcg
    20941 gttgctgaat cagaattaac ggatcaaaca acatcccgac ggagacaaaa aacagcaccg
    21001 caaacgcgtc gcgcaatggc agcgtatcgt gggcggcacg gtgactcagt tcagactcgt
    21061 tcagtaccat cccggcaaag aacgcaccga gtgcaaagga gacatcaaac agctctaccg
    21121 caccaaaggc aatccctaac gccagcgcca gcaccgacag ggtaaacagc tcgcgagaac
    21181 cggttgccgc gctgcgtgcc ataatccacg gcaccagacg gcgacctacc agcatcataa
    21241 tggcgataaa cgcgatcact ttgccgatgg tgatccccat atcgactgca agagtggcaa
    21301 agcccacatc gccctgttcc atcattcctg ccactgcggg cagcaacacc agcgtcagaa
    21361 ccattaccag gtcttccaca atcaaccaac cgatggcgat ttgcccacgc tgactgtcaa
    21421 ttaattgccg ttcttcaagt gcgcgcagta acaccacggt actggcggtg gaaagacata
    21481 aaccgaacac gataccggtc attaacgacc agcccagcac ggcagagagc gccataccca
    21541 gcagcgtcgc cacggctatc tgggcgatcg caccgggaat ggcgatggcc tttaccgcca
    21601 tcaaatcctt gagcgaaaag tgcagaccca cgccaaacat caacagaatg acgcccagtt
    21661 cagccagttc cggcgcaagc ttggtatcgg caacaaagcc cggagtgaat ggtcctgcca
    21721 gcacacccgc taacagatat cccaccagag gagaaatacg tagtttattg gccagcatgc
    21781 cgaggataaa ggcgagcaca aggccgccaa caatggtggt gataagcggg gtggcgtgat
    21841 gcattccgtc tccttttcct ggtggttatt gtccattttt ggccgggaaa accaaaatta
    21901 caggtaatag tttatgacaa tttcattgat gatgttcatg aataattatt gaattttgca
    21961 gaaaaatgga attagctgca aaaaagcacg gatcggaaaa cagaaggcgg tttaacagag
    22021 gaaaggctta tggcgactgt aaaacgatgc agccaaagtt tgcctttggc tgcaatgaaa
    22081 tcagtcttta tgccggttat caggcaggaa tatggtcaac atccccaata gtggcaggaa
    22141 agcacatatt ttatagacta actcgatgct ggtgtgatcg gcgataagcc ccagaactgc
    22201 cgctcccaga cctcccatgc cgaaagcaaa accgaaaaag agtccagaaa ccataccgat
    22261 acgtcctgga agcagctcct gagcgtagac cagaatggca gagaatgccg aagcgaggat
    22321 aaatccaata atcaccgtta aaacccccgt ccagtgcagg ctggcgtagg gtaaaatcag
    22381 cgtaaacggc gcaacgccga ggatagagcc ccaaatcaca tatttccgcc caattttatc
    22441 ccctacaggc ccgccgatca ccgtacctgc cgcaacggca aacaggaagg caaacagatg
    22501 aagctgagca ttctggatag ataatccgaa tttttgcatc agataaaagg tgtaatagct
    22561 gctgatgctc gccatataga aatatttcga gaaaatgagg attaacagaa tgctgaccgc
    22621 cagtaccact ttgttgcgcg gcagtggatt gataatcgtc gctttgggtt ttcctttatt
    22681 cattcggtgc tgtgccgagt accaacggct gatttgcgcc aacaccacga tcgccagcag
    22741 tgccgcaagc acaaaccagg caacgttgcc tttgccataa ggcgcgataa tcaccgccgc
    22801 cagcaagggt cccagggaac tgccaaagtt gccgccgacc tgaaagatag attgtgccag
    22861 gccatgccgc ccgccggaag ccatacgggc cacgcgagaa gattccggat gaaagaccga
    22921 tgaaccggta ccgaccagcg ccgccgccag cagaactgcg ccaaaactgc ccgccagcgc
    22981 aagcagcacc agaccactta aggtaaagca catgccaatt ggcaacgacc acggcatcgg
    23041 atatttatcg gtccagtagc cgaccactgg ttgcagtagc gaagaggcga gctggaaggt
    23101 gagggttatc atgccaatct gcataaatgt cagagaaaat tctgactgaa gcagaggata
    23161 aatcgccaga atcagcgatt ggatcatgtc gttcagcaga tgtgagaggc tgatagcacc
    23221 taaaatacca aacgatgttc gggccttggt cgttgacgca gccgcgcccg ccacaggctg
    23281 ggtttgttca ctcattgcca taggaaagtc actttttcag ggttgcgatg ttaagaatga
    23341 tcttatttgt gattattacc cgactaacat acctgtatgc gccgcctgaa ggaagtctca
    23401 acgctgaata cagaatttct aatctggatg cagatttatc ttcaccggac gcagacttgt
    23461 ctatgatgtc gcgtcatact atttttcaac acgttgaaat caggtcaggg agagaagtat
    23521 gaaattattg cagcggggcg tggcgttagc actgttaacc acatttacac tggcgagtga
    23581 agctgctctg gcgtatgagc aggataaaac ctacaaaatt acagttctgc ataccaatga
    23641 tcatcatggg catttttggc gcaatgaata tggcgaatat ggtctggcgg cgcaaaaaac
    23701 gctggtggat ggtatccgca aagaggttgc ggctgaaggc ggtagcgtgc tgctactttc
    23761 cggtggcgac attaacactg gcgtgcccga gtctgactta caggatgccg aacctgattt
    23821 tcgcggtatg aatctggtgg gctatgacgc gatggcgatc ggtaatcatg aatttgataa
    23881 cccgctcacc gtattacgcc agcaggaaaa gtgggcaaag ttcccgttac tttctgccaa
    23941 tatctaccag aaaagtaccg gcgagcgcct gtttaaacca tgggcgctgt ttaagcgtca
    24001 ggatctgaaa attgccgtta ttggtctgac gacggatgac acagcgaagt taggcaatcc
    24061 agaaaacttc accgatattg agttccgtaa gcccgccgat gaagcgaagc tggtgattca
    24121 ggagctgcaa cagacagaaa agccagacat tattatcgcg gcgacacata tggggcatta
    24181 cgataacggc gatcacggtt ctaacgcacc gggcgatgtg gagatggcac gcgcgttgcc
    24241 tgccggatcg ctggcgatga ttgtcggcgg tcactcgcaa gatccagtct gtatggcagc
    24301 ggaaaataaa aagcaggtag attacgttcc aggtacgcca tgcaaaccgg atcaacaaaa
    24361 cggcatctgg attgtacagg cgcatgagtg gggcaagtac gttggtcggg ccgactttga
    24421 gtttcgcaac ggcgaaatga aaatggttaa ctaccagctg attccggtga acctgaagaa
    24481 gaaagtgacc tgggaagacg ggaaaagcga gcgcgtgctg tacacccctg aaatcgctga
    24541 aaaccagcaa atgatctcgc tgttatcgcc gttccagaac aaaggcaaag cgcagctgga
    24601 agtgaaaata ggcgaaacca atggtcgtct ggaaggcgat cgtgacaaag tgcgttttgt
    24661 acagaccaat atggggcggt tgattctggc agcacaaatg gatcgcactg gtgccgactt
    24721 tgcggtgatg agcggaggcg gaattcgtga ttctatcgaa gcaggcgata tcagctataa
    24781 aaacgtgctg aaagtgcagc cattcggcaa tgtggtggtg tatgccgaca tgaccggtaa
    24841 agaggtgatt gattacctga ccgccgtcgc gcagatgaag ccagattcag gtgcctaccc
    24901 gcaatttgcc aacgttagct ttgtggcgaa agacggcaaa ctgaacgacc ttaaaatcaa
    24961 aggcgaaccg gtcgatccag cgaaaactta ccgtatggcg acattaaact tcaatgccac
    25021 cggcggtgat ggatatccgc gccttgataa caaaccgggc tatgtgaata ccggctttat
    25081 tgatgccgaa gtgctgaaag cgtatatcca gaaaagctcg ccgctggatg tgagtgttta
    25141 tgaaccgaaa ggtgaggtga gctggcagta atccgaaagt gccggatgtt cgcatccggc
    25201 acaatgctta atcgcggcgg gcgatatcag caaatttggc atcgaggatc ttcgccagat
    25261 cgcctgccgc cagttcgata tccagtccgc gcttgccgcc ggaaacataa atagtggcaa
    25321 attcttgtgc gggggcgtcg ataatcgttg gcagacgttt tttctgcccc agtgggctaa
    25381 tccccccaac caggtatccc gtcgaacgct gcgcgaccat cggatcggcc atctcaactt
    25441 tcttggcacc cagcgctttt gctacttttt taaggtccag ttgacctgcg accggcgtaa
    25501 cggccacggc aaggtgtttc atatcaccgt tcactgcgac cagcagcgtt ttgtagacct
    25561 gatccggatt caaacctaat tttttgacga cttcatcgcc aaaattggtt tcagccggat
    25621 cgtgctcgta ggtatggatt tgaaacgaaa tcttgttttt ttcgagtgat ttaactgcgg
    25681 gtgtcatggt aattttcctg ccttaaacaa aaaaacgaag caagcatacg cctgatggcg
    25741 gtccaaaaaa aagagtcatc ttgcctaaga gtattggcag gatggtgaga ttgagcgaca
    25801 atcgagttac accgtcgctt aaagtgacgg cataataata aaaaaatgaa attcctcttt
    25861 gacgggccaa tagcgatatt ggccattttt ttagcgcaac atttgcggta aattcccttc
    25921 tccatacagg tgtagtgcac cgaccgcgac cacatatcgc cccggcggca tggcgcgtaa
    25981 tttatcccgc caggcgagat ttcgctgatg catcagcaca tcgtacagcg actgactgaa
    26041 cgtattgggc agcgttattt cattattttg cggcggcgca ttcagccacc agctcatcat
    26101 ttgttgcagc aaccgtgcgt tggtatgcca gtgggtcagc gtatcgtcca gcagcgccag
    26161 tcctttgtca gggagctgga gcaacatggc aatctggttt tccgcccctt ccagttcaat
    26221 cacgggttta tgttgttgct tcgccgcctg caatagctga taatcgatac cgtattctgc
    26281 ccgcagcccc agtttttgtg cctgcgtcgc ctgaagaacc atcgcgattt gccacagcgg
    26341 ttgggtagaa aagagtgagg gagaaatgcc catctcctgg ctaatgtgct gcagtttttg
    26401 gagttgctcc tcgctaatgc gctcttccag cgcctcgcag gcaggcaaat tagcaaaagg
    26461 cgtatcgctg gtggaaacat ccgcctcgac gatcagcgca tcggcgtttt tgagcttttt
    26521 gagcaaacgg gtgggtaggg gagccatatc gtggctaccc atatgaatac tgccaatcag
    26581 atgaaaatgg cgattgccgg gaagagagat atcaatggca ggccaggtgt aatgattacc
    26641 gcgcagcgcg gcccaaagcg tttttacccg gtacaacaga tccatacgac ctccctttgt
    26701 gaaatatcat gctagcgcgc ggggagggat ggcgcaaatg gagttgggga cttgtttaat
    26761 gtgtttgtat gattcagtat gttcttgtat cgctatttac agggaagcaa cagttaaaaa
    26821 ctatgaaaca ggcaacaaga aaaccgacga cacccggaga tattcttctc tatgaatatc
    26881 tggaaccgct agatttgaaa atcaatgagt tagcagagtt gctgcatgtt catcgtaata
    26941 gcgtcagtgc actgatcaat aacaatcgta aactcaccac tgagatggca ttccgtctgg
    27001 cgaaagtttt tgataccaca gtcgattttt ggctaaacct ccaggcggca gttgatcttt
    27061 gggaagttga aaacaacatg cgcacccagg aagaattggg acggattgaa acagtggctg
    27121 aatatttggc acgccgtgaa gagcgtgcaa aaaaggtcgc gtaaggccaa aacatcgctt
    27181 catctcgtag gccggataag gcgttcacgc cgcatccggc atccgagcac cattgcctga
    27241 tgcgacgctt acgcgtctta tcaggcctac aaacctgtgc ctgaaccgta ggccggataa
    27301 ggcgtttacg ccgcatccgg cagtcatgcg tcgatgccaa atgcgccacc ctaaagcagc
    27361 gcatccgcaa tgacgtactt attccttcgg tttaaaccgc agcaaccggt tggcgttact
    27421 cactacggta atcgacgaga gcgccattgc cgctccggca actaccgggt taagcagtgt
    27481 tccagtaaac ggccacaaaa taccggcggc gaccggaata ccgatactgt tgtagataaa
    27541 cgcgccgagc aggttctgct tcatgttgcg cagcgttgcg cgggaaatag cgagcgcatc
    27601 cgcaacgccc atcaggctat ggcgcatcag ggtaatcgcc gcggtttcaa tggcaacatc
    27661 actgccgccg cccattgcaa tgccgacatc cgcctgggcc agcgctggcg catcgttaat
    27721 gccgtcgccc accattgcca cctgacgtcc ttcactttgc aggcgtttaa tcgcttcggc
    27781 tttaccgtcc ggcagcaccc cggcgatcac ctcatcaatc cctgcttctt tggcgatcgc
    27841 attggcggtg gttgggttat ccccggtcaa catcaccaga cgatatcccg ctttatgcag
    27901 acgttgcagc gccgccacgc tatcactacg caacggatcg cgtacagcca gcagggctac
    27961 cgctttcccg tcaaccgcca gcagcacagg cgttgcccct tgcgatgcct gagctgaaat
    28021 atctgcttca atcgctttag tatcaacttg ctggtcattt aacagcgcct gattgcccag
    28081 caataacgca tgaccttcag cttcaccgct cacgcccagc ccgcgcaatg tgcggaaacc
    28141 gttgacctgc ggcagctgca tatcgcttgc tttatcgagg atcgcccgcg ccagcggatg
    28201 gctggaacct tgttccagtg ccgccgccag acgcagcgcc tgcgcttcgt caaaatcagc
    28261 aaatgttttc actgcgacaa cctgcggctt cccttcggtc agcgtcccgg ttttatcgaa
    28321 cactacggta tcgagtgtac tagcgcgttg cagcgcgtcg gcgtcccgca ccagcacgcc
    28381 aaactcagcc gcccgcccga cgccggaaat aatcgacatc ggcgtcgcca gccccagcgc
    28441 acacggacag gcaataatca gcaccgtggt ggcaatcacc agggtataga caatctgcgg
    28501 tgccggacca aagaaatacc agattgccgc actgacaagc gcaataacca ctactaccgg
    28561 cacaaatacg gctgagattt tatccgccag ctgaccgatt tctggcttgc tgctctgggc
    28621 ctggcgcacc atacgaataa tccttgatag cgtggtatgg ctgccaaccg cactggcgcg
    28681 aaacagaacg ctaccgtcct gtaccactgt cccggcatgg acgctatcgc cttcgccttt
    28741 ttgctgcggg ataggttcgc ccgtcagcat cgcttcatcc agccatgctt cgccctgggt
    28801 aatctcgcca tctactggca cgcggtcgcc cgtcgtcagg cgcagtagca tgcctggctg
    28861 aacttccgcc agcggcacgc ttttttcacc ttcgtcagta accaggcgcg ccgtcggcgg
    28921 agttaaatcg agtaatttct ccagcgcctt tgaagaacgc tgacgtgcgc gcgcttccag
    28981 catatgaccg agattaatca gaccgataat catcgcactg gcttcatagt aaagatgccg
    29041 cgcttccatc gggaaccact gcggccacag gttgacgctc atcgaataga gccacgccac
    29101 acccgtaccc agcgccacca gcgtatccat cgtcgccgca ccgttcagca ggcttttcca
    29161 tgcactgcgg taaaaatggc cgccggcgaa aaccatcact gccagggtta tcaggccgat
    29221 aaccaaccac aggctgcggt tgtcagcggt gaccatcatg ttatcgccga tcatccccca
    29281 gaccattacc gggataccca cagccagtgc aacaattgcc tgccagcgga agcgcttcat
    29341 cgtagcgacg gcggtttctt gctggcgctc gcggcgtttc gcgtcatctt caatcgcttc
    29401 cgcgccgtag cccgcttttt ccaccgcctg cactaaatct tgtggggagg cactgcccat
    29461 caccagcgca gtacgctccg ccaggtttac ccgtgcctga gtgacgcccg gtacgctttg
    29521 cagcgcattt tgtacgcggg tgacacagct ggcgcagctc atgccgctca gcagcaactg
    29581 ctggctgtca tcgtcatcgg cggtcgctgc cggaagcgcc tcagaaaccg ctgtcagtgc
    29641 ttccgacggg attgatgact ccgccagcgg tttagccttt gggtggctta cagatgcgtc
    29701 ataacccgct tgtttgatgg tttcgatcag ctgttctgca ctggcagtcc cggtaacgtg
    29761 cgcttcagtg atagacacat ccgcttgttc cacatccggg cgctgttcga gactttcttt
    29821 cacgcgttta acgcagtgac cgcaggacag gccgtccagg gtcaggtcga tagtttgtga
    29881 cataaaacac tcctttaaga cagttttgac tggctgtgat aaaggttaaa ccttccagca
    29941 aggggaaggt caagaaatta ataacccagg cgagtaaaag tccgtaaaga ttaaaaaatc
    30001 ggctcgattt gcatcaggat tagacattta tctctttgtt ttcctgtagt taagttgcgg
    30061 gtgctaagtt aaatcaggat gcctgaaaat cggcaccggg gtgaggaatt acctcccgca
    30121 tctataaaaa ggagttaaca aaagatgtta gatgcaaaca aattacagca ggcagtggat
    30181 caggcttaca cccaatttca ctcacttaac ggcggacaaa atgccgatta cattcccttt
    30241 ctggcgaatg tgcctggtca actggcagca gtggctatcg tgaccagcga tggcaacgtc
    30301 tatagtgcgg gtgacagtga ttaccgcttt gcgctggaat ccatctcgaa agtctgtacg
    30361 ttagcccttg cgttagaaga tgtcggcccg caggcggtac aggacaaaat tggcgctgac
    30421 ccgaccggat tgccctttaa ctcagttatc gccttagagt tgcatggcgg caaaccgctg
    30481 tcgccactgg taaatgctgg cgctattgcc accaccagcc tgattaacgc tgaaaatgcc
    30541 gaacaacgct ggcagcgaat tttacatatc caacagcaac tggctggtga gctggtagcg
    30601 ctctctgacg aagtcaacca gtcggaacaa acaaccaact tccataaccg ggccattgcc
    30661 tggctgctgt actccgccgg atatctctat tgtgatgcaa tggaagcctg tgacgtgtac
    30721 acccgtcagt gctccacgct catcaatact gttgaactgg caacgcttgg cgcgacgctg
    30781 gcggcgggtg gtgtgaatcc gttgacgcat aaacgcgttc ttcaggccga caacgtgccg
    30841 tacattctgg ccgaaatgat gatggaaggg ctgtatggtc gctccggtga ctgggcgtat
    30901 cgtgttggtt taccgggcaa aagcggtgta ggtggcggta ttctggcggt cgtccctgga
    30961 gtgatgggaa ttgccgcgtt ctcaccaccg ctggacgaag aaggcaacag tgttcgcggt
    31021 caaaaaatgg tggcatcggt cgctaagcaa ctcggctata acgtgtttaa gggctgatca
    31081 tgatgaacac ggaaggtaat aacggtaaca aacccctcgg tctatggaac gtcgtttcca
    31141 tcggcattgg ggcaatggtg ggggcgggga tcttcgcgct gctggggcag gctgcgttgc
    31201 taatggaagc ctcgacctgg gtcgcctttg cttttggcgg tattgtggcg atgttttccg
    31261 gttatgccta tgcgcgtctg ggggcgagct atcccagcaa tggcggcatt atcgacttct
    31321 ttcgtcgcgg attaggcaac ggcgtctttt cgctggcgct ctcgttactg tacctgttga
    31381 cgctggcggt gagcatcgcc atggtcgccc gtgcttttgg cgcttatgcc gtgcagtttt
    31441 tgcatgaagg cagccaggag gagcacctta ttttgctcta cgcgttgggg atcattgcgg
    31501 tgatgacgct tttcaactcc ttaagcaacc atgcggtagg gcggctggag gtgatcctcg
    31561 tcggcattaa aatgatgatc ctgttattgc tgattattgc cggtgtctgg tcgctgcaac
    31621 cggcgcatat ttccgtctct gcgcccccca gctccggtgc gttcttctcc tgtatcggga
    31681 taaccttcct tgcctatgcg ggctttggca tgatggcgaa cgcggcggat aaagtgaaag
    31741 atccgcaggt cattatgcca cgggcgtttc tggtggcaat tggcgttacc acgttgcttt
    31801 atatctcgct ggcgctggtt ttgcttagcg atgtatcggc attagagtta gaaaaatatg
    31861 ccgataccgc cgtagcgcag gctgcttctc cgctgctcgg gcatgtgggt tatgtgatcg
    31921 tcgtcatcgg cgctttactg gcgacggctt cagccatcaa cgcgaacctg ttcgccgtgt
    31981 ttaacatcat ggacaacatg ggcagcgaac gcgaactgcc gaagctaatg aataaatccc
    32041 tgtggcggca gagtacctgg ggcaacatca ttgtcgtggt gttgattatg ctgatgacgg
    32101 cggcactaaa tttaggctca ctcgccagcg ttgccagcgc cacctttttg atttgctatc
    32161 tggcggtgtt tgtggtggcg atccgcctgc gtcatgatat tcacgcctcg ttgccgattc
    32221 ttatcgtagg tacgttggtg atgttgttgg tgatcgttgg ctttatctac agcctgtggt
    32281 cccagggcag ccgtgcgttg atatggatta ttggtgcaat cttactcagc cttattgtgg
    32341 caatggtcat gaagcgcaat aaaaccgtat aacacctctc tgcgcgcagt actgcctgta
    32401 ctgcgccttt gtctttaatt caaattacct ttcttattcc tttaataaat aagtcacaaa
    32461 aaaatgatat atacgcgata aatctgtgat gctaatcact tattaccgtg gattattttt
    32521 aatgtgtatg atggtaatct aaaagtcgta gtgtattcta aataaataac gtataagctt
    32581 tttag
//
LOCUS       DBFIFM010000032        32201 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.032, whole genome shotgun sequence.
ACCESSION   DBFIFM010000032 DBFIFM010000000
VERSION     DBFIFM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.032"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(52..1812)
                     /gene="ycaO"
                     /locus_tag="N7C74_001328"
     CDS             complement(52..1812)
                     /gene="ycaO"
                     /locus_tag="N7C74_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HGU2431226.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDERLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1942..2634
                     /locus_tag="N7C74_001329"
     CDS             1942..2634
                     /locus_tag="N7C74_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HGU2431227.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKSGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2833..3921
                     /gene="serC"
                     /locus_tag="N7C74_001330"
     CDS             2833..3921
                     /gene="serC"
                     /locus_tag="N7C74_001330"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HGU2431228.1"
                     /translation="MAQIFNFSSGPAMLPVEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NVACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            3992..5275
                     /gene="aroA"
                     /locus_tag="N7C74_001331"
     CDS             3992..5275
                     /gene="aroA"
                     /locus_tag="N7C74_001331"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HGU2431229.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5444..6208
                     /gene="ycaL"
                     /locus_tag="N7C74_001332"
     CDS             5444..6208
                     /gene="ycaL"
                     /locus_tag="N7C74_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HGU2431230.1"
                     /translation="MKNTKLLLAIVTSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6381..7064
                     /gene="cmk"
                     /locus_tag="N7C74_001333"
     CDS             6381..7064
                     /gene="cmk"
                     /locus_tag="N7C74_001333"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HGU2431231.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7175..8848
                     /gene="rpsA"
                     /locus_tag="N7C74_001334"
     CDS             7175..8848
                     /gene="rpsA"
                     /locus_tag="N7C74_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HGU2431232.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9008..9292
                     /gene="ihfB"
                     /locus_tag="N7C74_001335"
     CDS             9008..9292
                     /gene="ihfB"
                     /locus_tag="N7C74_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HGU2431233.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9499..11763
                     /locus_tag="N7C74_001336"
     CDS             9499..11763
                     /locus_tag="N7C74_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HGU2431234.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILLAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GQRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVVILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILDQGLWFLADRSLALLFLGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGRNDKGNNHSCVVK
                     VDDGTNSILLTGDIEAPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYTWLDTPHQGQVTVDFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11800..13548
                     /gene="msbA"
                     /locus_tag="N7C74_001337"
     CDS             11800..13548
                     /gene="msbA"
                     /locus_tag="N7C74_001337"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HGU2431235.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13545..14531
                     /gene="lpxK"
                     /locus_tag="N7C74_001338"
     CDS             13545..14531
                     /gene="lpxK"
                     /locus_tag="N7C74_001338"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HGU2431236.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            14568..15800
                     /gene="ycaQ"
                     /locus_tag="N7C74_001339"
     CDS             14568..15800
                     /gene="ycaQ"
                     /locus_tag="N7C74_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HGU2431237.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEYPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     QGIFREQWLADYYRLKRPALAAWREARAEQRQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGHC
                     PQGLFTDCRTGWEIDPVA"
     gene            15852..16034
                     /gene="ycaR"
                     /locus_tag="N7C74_001340"
     CDS             15852..16034
                     /gene="ycaR"
                     /locus_tag="N7C74_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HGU2431238.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16031..16777
                     /gene="kdsB"
                     /locus_tag="N7C74_001341"
     CDS             16031..16777
                     /gene="kdsB"
                     /locus_tag="N7C74_001341"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HGU2431239.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAHEVPGTGVDTPEDLERVRAEMR"
     gene            16931..17824
                     /locus_tag="N7C74_001342"
     CDS             16931..17824
                     /locus_tag="N7C74_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HGU2431240.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17801..18580)
                     /gene="elyC"
                     /locus_tag="N7C74_001343"
     CDS             complement(17801..18580)
                     /gene="elyC"
                     /locus_tag="N7C74_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HGU2431241.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            18716..19501
                     /gene="cmoM"
                     /locus_tag="N7C74_001344"
     CDS             18716..19501
                     /gene="cmoM"
                     /locus_tag="N7C74_001344"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HGU2431242.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPTQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19498..20820
                     /gene="mukF"
                     /locus_tag="N7C74_001345"
     CDS             19498..20820
                     /gene="mukF"
                     /locus_tag="N7C74_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HGU2431243.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALSYANADRLLDMRDEEMALR
                     DEEVTGELPPDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20801..21505
                     /gene="mukE"
                     /locus_tag="N7C74_001346"
     CDS             20801..21505
                     /gene="mukE"
                     /locus_tag="N7C74_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HGU2431244.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDIDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21505..25965
                     /gene="mukB"
                     /locus_tag="N7C74_001347"
     CDS             21505..25965
                     /gene="mukB"
                     /locus_tag="N7C74_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HGU2431245.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26226..28073
                     /gene="ldtD"
                     /locus_tag="N7C74_001348"
     CDS             26226..28073
                     /gene="ldtD"
                     /locus_tag="N7C74_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU2431246.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGILPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQQALDKGQLPTFVAGLAPQHPQYA
                     VMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28254..28802
                     /locus_tag="N7C74_001349"
     CDS             28254..28802
                     /locus_tag="N7C74_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HGU2431247.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28829..29476
                     /gene="gloC"
                     /locus_tag="N7C74_001350"
     CDS             28829..29476
                     /gene="gloC"
                     /locus_tag="N7C74_001350"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HGU2431248.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29527..30717)
                     /gene="aspC"
                     /locus_tag="N7C74_001351"
     CDS             complement(29527..30717)
                     /gene="aspC"
                     /locus_tag="N7C74_001351"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HGU2431249.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRIAADFLAKNTSVKRVWVSNPSWPNHKSVFNSADLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(30902..31990)
                     /gene="ompF"
                     /locus_tag="N7C74_001352"
     CDS             complement(30902..31990)
                     /gene="ompF"
                     /locus_tag="N7C74_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HGU2431250.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQLLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNISGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7846 a         7836 c         8650 g         7869 t
ORIGIN      
        1 taatataact cagacgaatc attaagccta cattacgtag gctatttgat tttattttgc
       61 ccagaatgct gctttggcgc gctgcagctt ttcgtaggcc ttcaacagcg actgatgtgc
      121 agcaaacgcg tgcagatcgc tatctactgg ttgcaagcca taaaacgccg cttcgccgct
      181 cattgccgca ctggcggctt ccacggcatc tgcgccgtac atgcgaacaa acgcattcag
      241 atattgcagc ggctggcgat cttcttcctg cgccagtaat aacaacgttt gcaggcagcg
      301 ataatagttg gcgcgttccg ggctgaatac cgatgagtta aactccatcg tccattcggt
      361 ccagaccaga gcctgttcca gatcgccacc agccagcgcc agcatggctt ttaattcacc
      421 aatacgcagg gtgtaccaac cgttatccga cccggtcgcc agacccaaca gctcacgcac
      481 gcgggtaaag tcatcaaaac cttcttcatc cagttgctcg atgaggttca ggtaatcttc
      541 tttttcccac tcgctgcctg gtagcgaaag aatcgtttca cgtaaatggc tgcccatact
      601 gttattcgcg agccacagat cttcagccgg ataaatatcg gacatgccag gcacgataat
      661 acggcaagca taaacgccca gatgctcgta atcggcaata taaacttctt tatcttcttt
      721 gttgaagata gccatcagcg tagcaaactc ttcttccgtg gtgccggaga aattccagtc
      781 cacaaacgga taatcggcat cctgcttgaa caggtcccag gagattaaac cgctggaatc
      841 gataaagtgc gtttcgaggt tggtatgttc agcgacttct tcatcatcga aggttggcgg
      901 agtaaacaca tccaaatctt tcaggccacg accttgcagc agctcggtca cggtacgttc
      961 cagtgctacg ccaaaatcag gatgcgcacc gaaagaggca aagcaggtgc cgttagcagg
     1021 attaaacagt accacgcaaa tcaccggata ctggccgcca agggaaccat catatgcgaa
     1081 gatcgggaaa ccttctgctt ccagtgtttc gatcgcttca accaccgctg ggtaacgcgc
     1141 cagcacgtct gccgggattt ccggcaagct gatgctttca gcaataatgc ggtttttcac
     1201 gtagcgttcg aaaacttcgg acaacccctg aacgcgtgct tcgttacggg tattgccagc
     1261 agacataccg ttagaaacgt acaggttacc aatgatattc atcggaatat aaacggtctg
     1321 attatcagac tgacgtgtaa acggcagacc gcaaatacca cgatcttcgt taccggattg
     1381 taggtcaatc agcatactgc cggtcaattc attctccgga tcgtaaaatg cgcgcagacg
     1441 ttcatcgagc agcccttctg gcacatcgtc attttcggtc agtgggaacc atttttcgtt
     1501 gggataatgc acaaatggac cgttggcgat ggtttcgccc agccagaaat cagcaaaaaa
     1561 gtagttggtt gagagacgct cgaaatattc accgagtgca gaagccagcg ccgctttctt
     1621 ggttgcgcct ttaccgttgg taaaacacag tgcgcactct ttgtcgcgaa tatgtacaga
     1681 ccagacgtta ggcacgggat tcagccagga ggcctcttca atctgaaagc cgaggtctga
     1741 aagtttttgc tggaagcgag cgatggaatc ttccagtgcg gcatctttgc cggggataaa
     1801 tgtttgcgtc atgaaaatca ctttagtcgt acggaaagcg cgcaataata cgggttttat
     1861 ctcaaaggcg ctatcaccgc cgccatgccg atgaactgtt gactatgctt ttagcggata
     1921 aaccacgtta agagcataaa aatgaaggcg ttcgatctcc accgtatggc atttgataaa
     1981 gtgccttttg atttccttgg cgaagttgca ctacgtagtc tttatacctt tgtactggtc
     2041 tttttgttcc tcaaaatgac cggaagacgc ggtgtgcggc agatgtcgct gtttgaagtt
     2101 ttaatcattc tgacgctggg atcagcggcg ggagatgtgg cgttttatga tgatgtgccg
     2161 atggtcccgg tacttatcgt ctttattact ctggcgttgt tataccgcct ggtaatgtgg
     2221 ttgatggcgc acagtgaaaa actggaagat cttctggaag gcaagccagt tgtcattatt
     2281 gaagatggcg agctggcctg gtcgaaactc aataactcca acatgacgga atttgagttc
     2341 tttatggagc tacgattgcg tggcgtggag cagctggggc aggtacgtct ggcgattctc
     2401 gaaaccaacg ggcaaatcag tgtctatttc tttgaagatg acaaggtgaa atcgggttta
     2461 cttattttac ccagtgattg tacgcagcgt tacaaagtgg tgccggagtc ggcggactat
     2521 gcctgtattc gttgtagtga aatcattcat atgaaagcgg gggaaaaaca attatgtccg
     2581 cgctgtgcaa atccagaatg gacgaaggca agtcgggcaa aacgggtgac ctgacagtaa
     2641 aaacatcggc tttttgctaa taatccgaga gattcttttg tgtgatgcaa gccacatttt
     2701 tgccctcaac ggttttactc attgcgatgt gtgtcactga atgataaaac cgatagccac
     2761 aggaataatg tattacctgt ggtcgcaatc gattgaccgc gggttaatag caacgcaacg
     2821 tggtgagggg aaatggctca aatcttcaat tttagttctg gtccggcaat gctaccggta
     2881 gaggtgctta aacaggctca acaggaactg cgcgactgga acggtcttgg tacgtcggtg
     2941 atggaagtga gtcaccgtgg caaagagttc attcaggttg cagaggaagc cgagaaggat
     3001 tttcgcgatc ttcttaatgt cccctccaac tacaaggtat tattctgcca tggcggtggt
     3061 cgcggtcagt ttgctgcggt accgctgaat attctcggtg ataaaaccac cgcagattat
     3121 gttgatgccg gttactgggc ggcaagtgcc attaaagaag cgaaaaaata ctgcacgcct
     3181 aatgtctttg acgccaaagt gactgttgat ggtctgcgcg cggttaagcc aatgcgtgaa
     3241 tggcaactct ctgataatgc tgcttatatg cattactgcc cgaatgaaac catcgacggt
     3301 atcgccatcg acgaaacgcc agacttcggc aaagatgtgg tggtcgccgc cgacttctct
     3361 tcaaccattc tttcccgtcc gattgacgtc agccgttatg gtgtaattta cgctggcgcg
     3421 cagaaaaata tcggcccggc aggcctgaca atcgtcatcg tccgtgaaga tttgctgggc
     3481 aaagcgaatg tcgcgtgtcc gtcgattctg gattattcca tcctcaacga taacggctcc
     3541 atgtttaaca cgccgccgac atttgcctgg tatctatctg gtctggtctt taaatggctg
     3601 aaagcgaacg gcggtgtagc tgaaatggat aaaatcaatc agcaaaaagc agaactgcta
     3661 tatggggtga ttgataacag cgatttctac cgcaatgacg tggcgaaagc taaccgttcg
     3721 cggatgaacg tgccgttcca gttggcggac agtgcgcttg acaaattgtt ccttgaagag
     3781 tcttttgctg caggccttca tgcactgaaa ggtcaccgtg tggtcggcgg aatgcgcgcg
     3841 tctatttata atgccatgcc gctggaaggc gttaaagcgc tgacagactt catggttgag
     3901 ttcgaacgcc gtcacggtta atgccgaaat tttgcttaat ccccacagcc agcctgtggg
     3961 gtttttattt ctgttgtaga gagttgagtt catggaatcc ctgacgttac aacccatcgc
     4021 tcgtgtcgat ggcactatta atctgcccgg ttccaagagc gtttctaacc gcgctttatt
     4081 gctggcggca ttagcacacg gcaaaacagt attaaccaat ctgctggata gcgatgacgt
     4141 gcgccatatg ctgaatgcat taacagcgtt aggggtaagc tatacgcttt cagccgatcg
     4201 tacacgttgc gaaattatcg gtaacggcgg tccattacac gcagaaggtg ccctggagtt
     4261 gttcctcggt aacgccggaa cggcaatgcg tccgctggcg gcagctcttt gtctgggtag
     4321 caatgatatt gtgctgaccg gtgagccgcg tatgaaagag cgcccgattg gtcatctggt
     4381 ggatgcgctg cgcctgggcg gggcgaagat cacttacctg gaacaagaaa attatccgcc
     4441 gttgcgttta cagggcggct ttactggcgg caacgttgac gttgatggct ccgtttccag
     4501 ccaattcctc accgcactgt taatgactgc gcctcttgcg ccggaagata cggtgattcg
     4561 tattaaaggc gatctggttt ctaaacctta tatcgacatc acactcaatc tgatgaagac
     4621 gtttggtgtt gaaattgaaa atcagcacta tcaacaattt gtcgtaaaag gcgggcagtc
     4681 ttatcagtct ccgggtactt atttggtcga aggcgatgca tcttcggctt cttactttct
     4741 ggcagcagca gcaatcaaag gcggcactgt aaaagtgacc ggtattggac gtaacagtat
     4801 gcagggtgat attcgctttg ctgatgtgct ggaaaaaatg ggcgcgacca tttgctgggg
     4861 cgatgattat atttcctgca cgcgtggtga actgaacgct attgatatgg atatgaacca
     4921 tattcctgat gcggcgatga ccattgctac ggcggcgtta tttgcaaaag gcaccaccac
     4981 gctgcgcaat atctataact ggcgtgttaa agaaaccgat cgcctgtttg cgatggcaac
     5041 agaactgcgt aaagtcggtg cggaagtaga agaggggcac gattacattc gtatcactcc
     5101 accggaaaaa ctgaattttg ccgagatcgc gacatacaat gatcaccgga tggcgatgtg
     5161 tttctcgctg gtggcgttgt cagatacacc agtgacgatt cttgatccca aatgtacggc
     5221 caaaacattt ccggattatt tcgagcagct ggcgcggatt agccaggcag cctgaatgaa
     5281 taacgtccaa taaatagcca aaactttctt tatcaaaacg tcggcacatt gccggcgttt
     5341 tttttcggac cttgtgagtc attttgatta atggtagcgt cgcttgtcaa tgtaagttgt
     5401 tgatacataa tgtttatata tgattaatca acggatgatt cacatgaaga atactaaatt
     5461 actgctggcg attgtgacct ctgcagcatt actgacgggg tgtcaaaata cccacggtat
     5521 tgataccaat atggctatca gctccggttt aaatgcctat aaagcagcaa cgttaagcga
     5581 tgccgatgca aaagcgattg ccaatcaggg ctgtgccgaa atggacagcg gcaatcaagt
     5641 cgcaagtaaa tccagcaagt acggtaaacg tctggcaaaa atcgccaaag cattgggtaa
     5701 caatattaat ggcacgccgg tcaactataa ggtttatatg accagcgacg tcaacgcatg
     5761 ggcgatggcg aacggctgtg ttcgtgttta cagtggcctg atggacatga tgaacgataa
     5821 cgaaattgaa ggcgttctgg gccatgaact gggccacgtc gcgttgggtc actcgctggc
     5881 tgaaatgaaa gcttcttatg cgatcgttgc cgcacgcgat gccatttcag ctaccagcgg
     5941 tgtggcttcc cagctttccc gctcacaatt gggtgatatc gcagaaggcg ctatcaatgc
     6001 gaaatactcc cgcgataaag agtccgaagc agatgatttc tcctttgatc tgttgaagaa
     6061 acgtggcatc agcacccagg ggctggttgg cagctttgaa aaactggcta gcctggatgg
     6121 cggtcgcacc cagtccatgt ttgactctca cccaccatca acagagcgtg cgcaacacat
     6181 ccgtgatcgt atcgcctctg gtaagtaaat cattgtcatc tttcgggctg gtcttctgcc
     6241 agcccgctat aattgcgcaa taaatcccca tctgaataca gacaaaactg gtttttgcac
     6301 acaacgttaa cgatttgtgg cgtcggcgcg tataatgcgc gcggttatgt taacggtacg
     6361 cctgttttaa ggagataaag atgacggcaa ttgccccggt tattaccatt gatggcccaa
     6421 gcggtgcagg gaaaggcacc ttgtgtaagg ctatggcgga agcgttgcaa tggcatctgc
     6481 tggattcggg tgcaatttat cgcgtactgg cactagcggc attacatcac catgttgatg
     6541 ttgcatcgga agatgcgctg gtgccgctgg catcccatct ggatgtacgt tttgtgtcaa
     6601 ccaatggcaa tctggaagtg atcctcgaag gggaagatgt cagcggcgaa attcgtactc
     6661 aggaagtggc gaatgcagct tcacaagtcg cggcattccc acgcgttcgt gaagcattat
     6721 tgcgtcgcca acgcgcgttt cgcgaattac caggcctgat tgccgatggc cgcgacatgg
     6781 gaacggtggt attccctgat gcgccagtga aaattttcct tgacgcctcc tcggaagaac
     6841 gtgcgcatcg ccgcatgcta cagttgcagg agaagggctt tagtgttaac tttgagcgcc
     6901 ttttggccga gatcaaagaa cgcgacgacc gcgatcgtaa ccgagcggta gcgccactgg
     6961 ttccggcagc cgatgcttta gtgttggatt ccaccacctt aagcattgag caagtgattg
     7021 aaaaagcgct acaatacgcg cgccagaaat tggctctcgc ataagcgacc gaatttgcag
     7081 tacccccgtt gcaatggaat gacagcgggt atgttaaaca accccatccg gcatggagcc
     7141 aggtggacgt taaatataaa cctgaagatt aaacatgact gaatcttttg ctcaactctt
     7201 tgaagagtcc ttaaaagaaa tcgaaacccg cccgggttct atcgttcgtg gcgttgttgt
     7261 tgctatcgac aaagacgtag tactggttga cgctggtctg aaatctgagt ccgccatccc
     7321 ggctgagcag ttcaaaaacg cccagggcga gctggaaatc caggtaggtg acgaagttga
     7381 cgttgctctg gacgcagtag aagacggctt cggtgaaact ctgctgtccc gtgagaaagc
     7441 taaacgtcac gaagcctgga tcacgctgga aaaagcttac gaagatgctg aaactgttac
     7501 cggtgttatc aacggcaaag ttaagggcgg cttcactgtt gagctgaacg gtattcgtgc
     7561 gttcctgcca ggttctctgg tagacgttcg tccggtgcgt gacactctgc acctggaagg
     7621 caaagagctt gaattcaaag taatcaagct ggatcagaag cgcaacaacg ttgttgtttc
     7681 tcgtcgtgcc gttatcgaat ccgaaaacag cgcagagcgc gatcagctgc tggaaaacct
     7741 gcaggaaggc atggaagtta aaggtatcgt taagaacctc actgactacg gtgcattcgt
     7801 tgatctgggc ggcgttgacg gcctgctgca catcactgac atggcctgga aacgcgttaa
     7861 gcatccgagc gaaatcgtca acgtgggcga cgaaatcact gttaaagtgc tgaagttcga
     7921 ccgcgaacgt acccgtgtat ccctgggcct gaaacagctg ggcgaagatc cgtgggtagc
     7981 tatcgctaaa cgttatccgg aaggtaccaa actgactggt cgcgtgacca acctgaccga
     8041 ctacggctgc ttcgttgaaa tcgaagaagg cgttgaaggc ctggtacacg tttccgaaat
     8101 ggattggacc aacaaaaaca tccacccgtc caaagttgtt aacgttggcg atgtagtgga
     8161 agttatggtt ctggatatcg acgaagaacg tcgtcgtatc tccctgggtc tgaaacagtg
     8221 caaagctaac ccgtggcagc agttcgcgga aacccacaac aagggcgacc gtgttgaagg
     8281 taaaatcaag tctatcactg acttcggtat cttcatcggc ctggacggcg gcatcgacgg
     8341 cctggttcac ctgtctgaca tctcctggaa cgttgcaggc gaagaagcag ttcgtgaata
     8401 caaaaaaggc gacgaaatcg ctgcagttgt tctgcaggtt gacgcagaac gtgaacgtat
     8461 ctccctgggc gttaaacagc tcgcagaaga tccgttcaac aactgggttg ctctgaacaa
     8521 gaaaggcgct atcgtaaccg gtaaagtaac tgcagttgac gctaaaggcg caaccgtaga
     8581 actggctgac ggcgttgaag gttacctgcg tgcttctgaa gcatcccgtg accgcgttga
     8641 agacgctacc ctggttctga gcgttggcga cgaagttgaa gctaaattca ccggcgttga
     8701 tcgtaaaaac cgtgcaatca gcctgtctgt tcgtgcgaaa gacgaagctg acgagaaaga
     8761 tgcaatcgca actgttaaca aacaggaaga tgcaaacttc tccaacaacg caatggctga
     8821 agctttcaaa gcagctaaag gcgagtaatt ctctgactct tcgggatttt tattctgaag
     8881 tttgttgagt ttacttgaca gattgcaggt ttcgtcctgt aatcaagcac taagggcggc
     8941 tacggccgcc cttaatcaat gcagcaacag cagccgctta atttgccttt aaggaaccgg
     9001 aggaatcatg accaagtcag aattgataga aagacttgcc acccagcaat cgcacattcc
     9061 cgccaagacg gttgaagatg cagtaaaaga gatgctggag catatggcct cgactcttgc
     9121 gcagggcgag cgtattgaaa tccgcggttt cggcagtttc tctttgcact accgcgcacc
     9181 acgtaccgga cgtaatccga agactggcga taaagtagaa ctggaaggaa aatacgttcc
     9241 tcactttaaa cctggtaaag aactgcgcga tcgcgccaat atttacggtt aagtttttac
     9301 tcaaacttga acgagagaaa agcacctgtc gggtgctttt ttcatttatc taatctggaa
     9361 ctggaagctg cctcgcagag ttttgagcag ttttcaccct ttcgttaaat tcttctgaat
     9421 gtgcctcggg gaacgcaaaa ttcccaccag acaaccgctc aacaaagttg cacactttcc
     9481 ataaacaggg aggggtgcat gaaaataacg acagtcggcg tatgcataat ttgcggaatt
     9541 tttccgttgc tgattttgcc ccaattgcct gggacattaa cccttgcgtt tctgactctc
     9601 ttcgcctgcg tactggcatt tatccctgtt aaaaccgtcc gttatatcgc gctgacgttg
     9661 ctgtttttcg tttggggcat attatcagca aagcaaattt tgttggcagg agaaacctta
     9721 actggcgcga cgcaggatgc aattgttgag atcactgcta ctgacggcat gaccactcat
     9781 tacggtcaaa ttacttatct acaaggtcaa cgtatattcc ctgcgccagg ccttgtgctg
     9841 tatggcgaat atcttccgca agcggtttgt gccggacaac tgtggtcaat gaaactcaaa
     9901 gttcgtgccg ttcatggtca acttaatgat ggcggctttg atagccagcg ttatgccatt
     9961 gcccagcatc agccgctcac cggccgcttt ctgcaggcaa gtgttattga accgaattgt
    10021 agcctgcgtg cacagtatct ggcgtcacta caaacaacgc tgcaacccta tccgtggaat
    10081 gcggttattc ttggtttagg tatgggggaa cggttatccg tccctaaaga aatcaaaaat
    10141 atcatgcgtg atactggaac ggcgcattta atggcgatat cgggattgca catcgctttt
    10201 gcggcgttgc tggctgccgg actcattcgc agtggacaaa tttttctgcc tgggcgctgg
    10261 atccactggc aaatgccatt aattggcgga atctgctgtg ctgcttttta tgcctggctg
    10321 acgggaatgc aacctcctgc attgcgtacc gttgtggcgc ttgctacgtg gggtatgctt
    10381 aagttaagtg ggcgacagtg gagtggctgg gatgtatgga tatgttgtct ggcggcaatt
    10441 ttgctgatgg atcctgttgt cattctctcg caaagtttat ggctctctgc cgctgcggtc
    10501 gcggcattga tattttggta tcagtggttt ccctgtcctg agtggcaact gccgccggta
    10561 ttgcgtgcag ttgtttccct catccatctg caactgggaa tcacacttct gcttatgccc
    10621 gtgcaaatcg tcatttttca tggcattagt ctgacctcgt ttattgcaaa tctattagca
    10681 attcccttgg tgacatttat cacggttccg ttgatcctcg ccgcgatggt tgtgcattta
    10741 agcgggccgt taatcctgga tcaagggtta tggtttcttg ccgaccggtc tttggcttta
    10801 cttttcttgg ggttaaagag tttgccagaa gggtggatca acattgctga acgttggcaa
    10861 tggctatcat tttccccatg gttcttactg gtggtatggc gattaaacgc ctggcgaacg
    10921 ttgccagcaa tgtgtgtggc tgtaggcttg ctgatgtgct ggccgctgtg gcaaaaacct
    10981 cgacctgacg agtggcaagt gtacatgctt gatgtcgggc aagggctggc aatggtgata
    11041 gccagaaacg gcaaagcgat tctctatgac acgggactgg cctggcctga aggggatagt
    11101 gggcaacaac tgattatccc ctggctccac tggcataatc ttgaaccgga aggcgttatc
    11161 ctgagtcatg aacatctgga tcaccgggga gggctggact caatattgca tacatggccg
    11221 atgttatgga tcagaagtcc gttaaactgg gaacatcatc agccctgtgt gcgtggcgaa
    11281 gcgtggcaat ggcaaggatt gcgtttcagc gcgcactggc ctttacaagg tcgcaacgat
    11341 aaaggaaata accattcctg tgtggttaag gttgatgacg ggacgaatag cattcttcta
    11401 accggtgata ttgaagcccc agctgaacaa aagatgctaa gccgttactg gcagcaagtg
    11461 caggcaacat tgcttcaggt acctcaccat ggcagtaata cctcatcatc attgccatta
    11521 attcagcgag tgaatggaaa agtggcactc gcatcggcat cgcgctataa cgcatggcga
    11581 ctgccctcta acaaagttaa gcatcgctat caacaacaag gttatacgtg gcttgatact
    11641 cctcatcagg ggcaagtaac ggtcgatttt tcagcgcaag gctggcggat tagcagcctc
    11701 agggagcaaa ttttacctcg ttggtatcat cagtggtttg gcgtgccagt ggataacggg
    11761 tagaatatgc ggctatttca acaaatgctg gttttttgaa tgcataacga caaagatctc
    11821 tctacgtggc agacattccg ccgactgtgg ccaaccattg cgccttttaa agcgggtctg
    11881 atcgtggcgg gcgtagcgtt aatcctcaac gcagccagcg ataccttcat gttatcgctc
    11941 cttaagccac ttcttgatga tggctttggt aaaacagatc gctccgtgct ggtgtggatg
    12001 ccgctggtgg tgatcgggct gatgattttg cgtggtatca ccagctatgt ctccagctac
    12061 tgtatctcct gggtatcagg aaaggtggta atgaccatgc gtcgccgcct gtttggtcac
    12121 atgatgggaa tgccggtttc attctttgac aaacagtcaa cgggtacgct gttgtcacgt
    12181 atcacctacg attccgaaca ggttgcttct tcctcttccg gcgcactgat tactgttgtg
    12241 cgtgaaggtg cgtcgatcat cggcctgttc atcatgatgt tctattacag ttggcaattg
    12301 tcgatcattt tgattgtgct ggcaccgatt gtttcgattg cgattcgcgt agtatcgaag
    12361 cgttttcgca acatcagtaa aaacatgcag aacaccatgg ggcaggtgac caccagcgct
    12421 gaacaaatgc tgaaaggcca taaagaagta ttgattttcg gtggtcagga agtggaaacg
    12481 aaacgcttcg ataaagtcag caaccgaatg cgtcttcagg ggatgaaaat ggtttcagcc
    12541 tcttccatct ctgatccgat cattcagctg atcgcctctt tggcgctggc gtttgttctg
    12601 tatgcggcga gcttcccaag tgtcatggat agcctgactg ccggtacgat taccgttgtt
    12661 ttctcttcaa tgattgcact gatgcgtccg ctgaaatcgc tgactaacgt taacgcccag
    12721 ttccagcgcg gtatggcggc ttgtcagaca ctgtttacca ttctggacag tgagcaggag
    12781 aaagacgaag gtaagcgcgt gatcgagcgt gcgactggcg acgtggaatt ccgcaacgtc
    12841 acctttactt atccgggacg tgacgtaccc gcattgcgta acatcaacct gaaaattccg
    12901 gcagggaaaa cggtcgctct ggttggacgc tctggttcag gtaaatcaac catcgccagc
    12961 ctgatcacgc gtttttacga tattgatgaa ggcgaaatcc tgatggatgg tcacgatctg
    13021 cgcgagtata ccctggcgtc gttgcgtaac caggttgctc tggtgtcgca gaatgtccat
    13081 ctgtttaacg atacggttgc taacaacatt gcttacgcac gaactgaaca gtacagccgt
    13141 gagcaaattg aagaagcggc gcgtatggcc tacgccatgg acttcatcaa taagatggat
    13201 aacggtctcg atacagtgat tggtgaaaac ggcgtactgc tctctggcgg tcagcgtcag
    13261 cgtattgcta tcgctcgagc cttgttgcgt gatagcccga ttctgattct ggatgaagct
    13321 acctcggctc tggataccga atccgaacgt gcgattcagg cggcactgga tgagttgcag
    13381 aaaaaccgta cctctctggt gattgcccac cgcttgtcta ccattgaaaa ggcagacgaa
    13441 atcgtggtcg tcgaggatgg tgtcattgtg gaacgcggta cgcataacga tttgcttgag
    13501 caccgcggcg tttacgcgca acttcacaaa atgcagtttg gccaatgatc gaaaaaatct
    13561 ggtctggtga atcccctttg tggcggctat tgctgccact ctcctggttg tatggcctgg
    13621 tgagtggcgc gatccgtctt tgctataaac taaaactgaa gcgcgcctgg cgtgcccccg
    13681 taccggttgt cgtggttggt aatctcaccg caggcggcaa cggaaaaacc ccggtcgttg
    13741 tctggctggt ggaacagttg caacagcgcg gtattcgcgt gggggtcgta tcgcggggat
    13801 atggtggtaa ggctgaatct tatccgctgt tattgtcggc agataccacc acagcacagg
    13861 cgggtgatga acctgtgttg atttatcaac gcactgatgc gcctgttgcg gtttctcccg
    13921 ttcgttctga tgcggtaaaa gccattctgg cgcaacaccc tgatgtgcag atcatcgtaa
    13981 ccgacgacgg tttacagcat taccgtctgg cgcgtgatgt ggaaattgtc gttattgatg
    14041 gtgtgcgtcg ctttggcaat ggctggtggt tgccggcggg gccaatgcgt gagcgagcgg
    14101 ggcgcttaaa gtcagttgat gcggtaatcg tcaacggcgg tgtcccccgc agcggtgaaa
    14161 tccccatgca tctgctgccg ggtcaggcgg tgaatttacg taccggtacg cgttgtgacg
    14221 ttgctcagct tgaacatgtg gtggcgatgg cagggattgg gcatccgccg cgcttttttg
    14281 ccacgctgaa gatgtgcggc gtacaaccgg aaaaatgtgt accgctggcc gatcatcagt
    14341 ctttgaacca tgcggatgtc agcgcgttgg taagcaccgg gcaaacgctg gtaatgactg
    14401 aaaaagatgc ggtgaaatgc cgggcctttg cagaagaaaa ttggtggtat ttgcccgttg
    14461 acgcacagct ttcaggtgat gaaccagcga aactgcttgc gcaactaacc tcgctggctt
    14521 ctggcaacta gttacgccgc ggcagcgttc gattgatgga gtcatgaatg tcgctgccgc
    14581 acctctccct tgctgatgcg cgtaatcttc accttgccgc gcaaggcctg ttaaacaaac
    14641 cccgccgtcg agcgtcgttg gaggatattc cggcaacgat ctcccgcatg tccttgctgc
    14701 aaatcgatac catcaatatt gttgcccgta gcccatatct ggtgcttttc agtcgtctgg
    14761 gaaattatcc tgcccagtgg ctggatgagt ctctggcgcg tggcgaatta atggaatact
    14821 gggcgcatga agcctgcttt atgccacgca gtgactttcg tcttattcgc caccgcatgc
    14881 tggcacctga aaaaatgggc tggaaataca aagacgcctg gatgcaggaa catgaggcgg
    14941 aaattgcaca gttaattcag catattcatg ataaggggcc ggtacgttca gccgattttg
    15001 agtatcctcg taaaggtgca agcggctggt gggaatggaa gccgcataaa cggcatctgg
    15061 aaggtttatt tactgccgga aaggtgatgg tgattgaacg gcgcaacttc cagcgcgttt
    15121 atgatttaac ccaccgtgtc atgcctgact gggatgatga gcgcgatctc gtttcgcaaa
    15181 cagaagcaga aatcatcatg ctggataaca gtgcgcgtag ccagggaata ttccgcgaac
    15241 agtggctggc agattactat cggctgaaac gtccggcact ggcagcgtgg cgcgaagcga
    15301 gggctgaaca gcggcaaatc attgctgtgc atgttgaaaa attgggcaat ctttggctgc
    15361 atgctgattt gctgccgcta ctcgagcgag cgctggccgg aaagctcact gcaacgcaca
    15421 gcgcggtact ttcgcctttt gatcctgttg tctgggatcg caaacgcgca gagcagcttt
    15481 ttgattttag ctaccggctg gagtgctata ccccagcgcc gaaacgccag tatggctatt
    15541 ttgttctgcc gttattacat cgtgggcaat tagttgggcg aatggatgcc aaaatgcatc
    15601 gccagacagg catccttgaa gttatctctc tgtggttaca ggaaggtatt aaaccaacga
    15661 caacgctgca aaaagggtta cgtcaggcga ttactgattt cgctaactgg cagcaggcaa
    15721 cgcgggtgac attaggacac tgcccgcaag gtctctttac ggattgccgc accggctggg
    15781 aaatagaccc cgtcgcataa atgattatga taagatttat gcattcataa cccgatccat
    15841 ctggaggaac tatggatcat cgtctgcttg aaatcattgc ctgcccggtt tgcaacggaa
    15901 aactttggta taaccaggaa aaacaagaac ttatttgcaa actggacaac ctggctttcc
    15961 cgctgcgcga tggcattcca gtgttgctgg aaacggaagc ccgcgtgctg actgctgatg
    16021 agagtaaatc atgagttttg tggtcattat tcccgcgcgc tacgcgtcca cgcgtctgcc
    16081 cggtaaacca ttggttgata ttaacggcaa acccatgatt gttcatgttc ttgaacgcgc
    16141 gcgcgaatca ggtgccgagc gcatcatcgt ggcaaccgat catgaggatg tcgcccgcgc
    16201 cgttgaagcc gctggcggtg aagtatgtat gacgcgcgcc gatcaccagt cagggacaga
    16261 acgcttggcg gaagttgtcg aaaaatgcgc gttcagcgac gacacggtga tcgttaatgt
    16321 gcagggtgat gaaccgatga tccctgcgac aatcattcgt caggttgctg ataacctcgc
    16381 tcagcgtcag gtgggtatgg cgactctggc ggtgccaatc cacaatgcgg aagaagcgtt
    16441 taacccgaat gcggtgaaag tggttctcga cgctgaaggg tatgcactgt acttctcacg
    16501 cgctaccatt ccgtgggatc gtgatcgttt tgcaaaaggc cttgaaaccg ttggcgataa
    16561 cttcctgcgt catcttggta tttatggcta ccgtgcaggc tttatccgtc gttacgtcac
    16621 ctggcagcca agcccgttag aacacatcga aatgttagag cagcttcgtg ttttgtggta
    16681 cggcgaaaaa atccatgttg ctgtcgcgca tgaagttcct ggcacaggtg tggatacccc
    16741 tgaagatctc gagcgcgttc gcgctgaaat gcgctaattt cacttcacga cacttcagcc
    16801 aattttggga ggagtgtcgt accgttacga ttttcctcaa tttttctttt caacaattga
    16861 tctcattcag gtgacatctt ttatattggc gctcattatg aaagcagtag cttttatgag
    16921 ggtaatctga atggaacagc tgcgtgccga attaagccat ttactgggcg aaaaactcag
    16981 tcgtattgag tgcgtcaatg aaaaagcgga tacggcgttg tgggctttgt atgacagcca
    17041 gggaaaccca atgccgctaa tggcaagaag ctttagtacg cccggaaaag cccgacaact
    17101 ggcatggaaa accaccatgc tggcaagaag tgggactgtc cgtatgccga ctatttatgg
    17161 tgtgatgacg catgaagaac accccggccc tgatgtcctg ctactggagc ggatgcgtgg
    17221 tgtttcggta gaggcaccag cccgaacacc agaacgttgg gaacagctca aagaccaaat
    17281 cgttgaagcc ttactggcct ggcaccgtca ggacagtcgc ggttgcgtcg gcgcggtcga
    17341 caatactcag gaaaatttct ggccctcatg gtaccggcaa catgttgaag tgctatggac
    17401 cacgctcaat cagttcaata acaccggtct gacgatgcag gataagcgga tcctgtttcg
    17461 cactcgcgaa tgtctcccgg cattatttga aggttttaac gacaattgtg tgctgattca
    17521 cggtaacttc tgtttacgca gcatgttgaa agattcgcgc agcgatcagt tactggcgat
    17581 ggtcgggccg ggactaatgc tttgggcacc gcgagaatac gaactgttcc gactaatgga
    17641 taattctctg gcggaagatt tgctctggag ttacctgcaa cgcgcgccag tggcggagtc
    17701 gttcatctgg cggcgttggt tgtatgtgtt atgggatgaa gttgcgcaac tggttaatac
    17761 cggacgattt agtcggcgca acttcgatct ggcatcaaaa tcactcttgc cgtggctcgc
    17821 ctgacgaacc ttttagccac tgccagatgc ggccaagcgt ttcatagcca acacgatcgc
    17881 tatgcatcaa ccagaccgga gaagggattg cccgttccca cgggttaagc ggcgagtcga
    17941 tggccaactg attcgccggg gctggtagtg gatttaaccc ttcctgctga aaaaagatca
    18001 tcgcccgcgg cagatgtgat gcggaggtca ccagcaggaa aggggcgtca ccaatcgcct
    18061 gtttcactgc cgcagcttct tcttcggtat cttttggcaa atccagggtg ataatttgct
    18121 cgcgcggtac gcccagcgat tgcgcaactc tggcacctac ttccgctgta ctcaccgtat
    18181 tggtttttgc tacgcctccc gtgaagatca gttttgaacc cggattttcg cgccataaac
    18241 ggataccctc attcaggcgt ggcagactgt tattgattaa attagagctc ggtgcccact
    18301 gcgggttcca ggtataacca cctccgagca ccacgatata gtccactttt tgcgaattat
    18361 tccatgtcgg ataggtgctt tcgatgggac gcagcaggcg gtcggctacc ggttgcaaac
    18421 tcaataacaa gagtgccagc cacccaatgc taataaaaat cttcccggtt ttttgaaaac
    18481 ggctaaacca cagtagcgcc aggccagcgc caataattaa caacatcagc ggaagtggca
    18541 gcaacatatt gccaatcact tttttcagtg taaaaagcat cctttctggt tcctttttta
    18601 accatatagc aagggatcgc cagcgatatt acaccagacg ggttcattct cgccgtggct
    18661 gtgacaaaat agcgtcttta tcagaaaacg tcttagccaa ccggtggaga aagcaatgca
    18721 ggatcgcaat tttgatgata ttgcggaaaa gttttcccgt aacatttacg gcaccaccaa
    18781 agggcagctt cgacaggcta ttctgtggca ggatctcgat cgcgtgctgg cggaaatggg
    18841 tccgcaaaaa ctgcgtgtgc tggatgcggg cggtggagaa gggcagaccg caatcaaaat
    18901 ggccgagcgc gggcatcagg tcattttatg cgatctttct gcgcagatga tcgaccgcgc
    18961 aaaacaggcg gcagaagcaa aaggtgtgag cgacaacatg caatttatac attgcgccgc
    19021 tcaggatgtt gcttcgcatt tggaaacgcc cgttgatctg atattgttcc atgcggtgct
    19081 cgagtgggtg gctgatcccc gcagcgtatt gcagaccctg tggtcagtgt tgcgtccagg
    19141 cggcgtgttg tcgttaatgt tctacaatgc gcatggtttg ttgatgcata acatggtcgc
    19201 cgggaatttt gattacgtgc aggcgggaat gccgaaaaag aaaaaacgga cgctttcgcc
    19261 agattatcca cgcgacccga cgcaggttta tctgtggctg gaagaagctg gttggcaaat
    19321 tatgggtaag acaggcgttc gcgtgtttca tgattatctg cgcgagaagc accagcagcg
    19381 cgactgctat gaagcattac ttgaattaga aacgcgttat tgccgtcagg aaccgtatat
    19441 taccctgggg cgttatattc atgtcaccgc gcgcaaaccg cagagcaagg ataaagtatg
    19501 agtgaatttt cccagacagt ccccgaactg gttgcctggg ccagaaaaaa tgacttctcc
    19561 atttcgctgc cggtagaccg actctctttt ctgctggcag ttgccacgct gaacggcgag
    19621 cgtctggatg gtgagatgag tgaaggcgag ctggtggatg cattccgcca tgtgagtgat
    19681 gcgtttgagc aaaccagcga aaccatcggc gtgcgcgcca acaacgcgat caacgacatg
    19741 gtgcgtcaac gtctgctgaa ccgctttacc agcgagcagg cagaagggaa cgcaatttac
    19801 cgtctgacgc cgctcggcat cggcattact gactattaca tccgtcagcg cgagttttct
    19861 acgctgcgcc tttctatgca gttgtcgatt gtggcgggtg agctcaaacg cgcagcagat
    19921 gccgccgaag agggcggtga tgaatttcac tggcaccgta atgtctatgc gccactgaaa
    19981 tattcggtag cagaaatttt cgacagtatc gacctgacgc aacgtctgat ggacgaacag
    20041 cagcagcagg tgaaggacga tatcgcccag ttgctgaaca aagactggcg ggcggcgatt
    20101 tccagctgtg aattgttgct ttcggaaact tccggaacgc tgcgtgaatt gcaggataca
    20161 ctggaagcgg caggcgacaa attgcaggct aatctgttgc gcattcagga tgcgacgatg
    20221 actcatgacg atctgcattt cgtcgatcgt ctggtgttcg atctgcagag caaactcgac
    20281 cgtattatca gttggggcca gcaatccatc gatttgtgga ttggctacga ccgccacgta
    20341 cataaattta ttcgtaccgc gatcgatatg gataaaaacc gcgtctttgc tcagcggtta
    20401 cgtcagtcgg tacaaaccta ttttgatgag ccgtgggcgc taagttatgc caatgccgat
    20461 cgtctgctgg atatgcgtga cgaagagatg gcactgcgcg atgaagaagt gactggggaa
    20521 cttccgccag atttggaata cgaagagttt aacgagatcc gcgaacagct ggcggcgatc
    20581 atcgaagaac aacttgccgt gtacaaaacc agacaagtgc cgctggatct tggtctggtg
    20641 gtacgcgaat atctgtcaca gtatccgcgt gcacgtcact ttgacgttgc gcgcattgtt
    20701 attgatcagg cggtacgtct tggcgtagcg caagcagatt tcaccggact gccagcgaaa
    20761 tggcagccga ttaatgatta cggagccaag gtacaggcgc atgtcatcga caaatattga
    20821 acaagtgatg ccggttaagc tggcgcaggc gctggcgaat ccgttatttc cggcgctgga
    20881 cagcgcctta cgttcaggcc gccatattgg cctcgacgaa ctggataatc atgcattcct
    20941 gatggatttt caggaatatc tggaagagtt ttacgcgcgt tataacgttg agcttattcg
    21001 tgcaccggaa gggttcttct atttacgtcc acgttccacc acgctgatcc ctcgttccgt
    21061 cttgtcggaa ctggatatga tggtcgggaa aatcctctgt tatctctatc tcagcccgga
    21121 acggctggcg aatgagggga ttttcaccca gcaggaactg tacgacgaac tgctcaccct
    21181 ggccgatgaa gcgaaactgc tgaaactggt gaacaaccgt tcaaccggtt cagacattga
    21241 ccgtcagaag ttgcaggaaa aagtacgttc ttcgctcaac cgtctgcgtc gtttaggtat
    21301 ggtgtggttt atgggccacg acagcagcaa gttccgcatt accgaatcgg tgttccgctt
    21361 cggagccgat gtgcgtgctg gcgacgatcc ccgtgaagca cagcgtcgcc tgattcgtga
    21421 tggcgaagca atgccgattg aaaatcatct gcaactcaac gatgaaaccg aagagaatca
    21481 gccagatagc ggagaggaag aataatgatt gaacgcggta aatttcgctc actgacgctg
    21541 attaactgga acggcttctt tgcccgaact tttgaccttg acgagctggt cacgacgctt
    21601 tctggcggta acggggcggg taaatccacc accatggcgg cgttcgttac ggcgctgatc
    21661 cccgacctga ccctgctgca tttccgtaac actacggaag ccggggccac cagcggttcg
    21721 cgcgataaag gtctgcacgg taagctgaaa gcgggtgtct gttattcgat gctcgacacc
    21781 attaactcgc gccaccagcg cgtggtggtc ggtgtgcgtc tgcaacaggt tgccggacgc
    21841 gatcgtaaag tggatatcaa accatttgcc attcagggac tgccgatgtc ggtgcagccg
    21901 acacagctgg tgaccgaaac tctgaacgaa cgccaggcgc gcgtgctgcc gcttaacgag
    21961 ctgaaagaca agctcgaggc gatggaaggc gtgcagttta aacagttcaa ctccattacc
    22021 gattaccact cgctgatgtt cgatctgggc atcattgcgc gtcgtctgcg ctccgcatct
    22081 gaccgtagca aattctatcg tctgatcgaa gcttcgctgt atggcgggat ctccagtgcc
    22141 attacccgtt ctctgcgcga ctacctgttg ccggaaaaca gcggcgtgcg taaagcgttc
    22201 caggacatgg aagcggcgct gcgtgaaaac cgcatgacgc tggaagcgat tcgtgtcacc
    22261 cagtcggacc gtgacctgtt taagcatctg atcagcgaag ctaccaacta cgtggcggcg
    22321 gactacatgc gtcacgccaa cgagcgccgt gtccatctcg ataaggccct ggagtttcgt
    22381 cgcgaactac atacttcgcg tcagcaactg gcggcggagc agtacaaaca cgtcgatatg
    22441 gcgcgtgagc tggcagagca caacggtgcc gaaggcgatc tggaagcgga ttatcaggcg
    22501 gccagcgatc acctgaacct ggtgcaaacc gcactgcgtc agcaggagaa gatcgaacgc
    22561 tacgaagcgg atctcgatga gctgcagatc cgtctggaag agcaaaacga agtggtggca
    22621 gaagccatcg aacgccagga agagaacgag gctcgcgcgg aagctgccga actggaagtg
    22681 gacgagctga aaagccagct tgctgactac cagcaggcgc tggacgtcca gcaaacgcgc
    22741 gcgatccagt acaaccaggc gattgctgcg cttaatcgtg ccaaagaact gtgccatctg
    22801 ccggacttaa ccgccgacag cgccgccgaa tggctggaaa ccttccaggc gaaagagctg
    22861 gaagcgactg aaaaaatgct ctctcttgag cagaaaatga gcatggcgca aaccgcgcac
    22921 agccagtttg agcaggctta tcagctggtg gtggcaatca acggcccgct ggcgcgtaac
    22981 gaggcgtggg atgtcgctcg cgaactattg cgcgaagggg tcgatcagcg tcacctggca
    23041 gagcaggttc agccgttgcg gatgcgctta agcgaactgg aacagcgtct gcgcgaacag
    23101 caagaagctg agcgtctgct ggcagatttc tgcaaacgtc agggcaagaa ttttgacatt
    23161 gacgagctgg aagccctgca tcaggagctg gaagcacgca ttgcctctct ttccgatagc
    23221 gtgtctaacg cccgtgaaga gcggatggca ctgcgccagg agcaggaaca gctgcagtct
    23281 cgcattcaga gtttgatgca gcgtgcaccg gtttggctgg cagcgcaaaa cagtctcaac
    23341 cagttgagcg aacagtgcgg cgaagagttc acctccagcc aggacgtcac tgaatatctg
    23401 caacagttgc tggagcgtga gcgcgaggcc attgttgaac gcgatgaagt aggcgcgcgc
    23461 aaaaacgccg tcgatgaaga gatcgaacgt ttaagccagc ctggcggctc tgaagatcag
    23521 cgtttgaacg cgctagcgga gcgttttggt ggtgtgctgc tgtcagaaat ttatgacgac
    23581 gttagcctgg aagatgcgcc gtacttctca gcgctgtatg gcccgtcacg ccacgccatc
    23641 gtggtgccag atctgtcaca ggtaaccgaa cacctggaag gtttgaccga ttgcccggaa
    23701 gatctctatt tgatcgaagg ggatccgcag tcattcgatg acagcgtgtt cagcgttgat
    23761 gagctggaaa aagcggtagt ggtgaaaatc gccgatcgtc agtggcgtta ttcacgtttc
    23821 ccggaagtgc cgctgtttgg tcgtgctgcg cgtgaaagcc gtattgaaag cctccatgcc
    23881 gagcgtgaag tgctttccga acgcttcgcc acgctctcct ttgatgtaca gaaaacccag
    23941 cgtctgcatc aggcgttcag ccgctttatc ggcagtcatc tggcggtggc gtttgagtct
    24001 gacccggaag cagaaatccg tcaactgaac agccgtcgcg tcgaactgga gcgggcgttg
    24061 agtaatcatg aaaatgataa ccagcagcag cgtattcagt ttgagcaggc gaaagaaggc
    24121 gttacggcgc tgaaccgcat tctgccgcgt ctcaacctgt tggctgatga cagcctggcg
    24181 gatcgcgtcg atgaaatccg cgaacgtctg gatgaagccc aggaagccgc gcgttttgtt
    24241 cagcagtttg gcaatcaact ggcgaaactg gaaccgatcg tttcggtatt gcagagcgac
    24301 ccggaacagt tcgaacagtt aaaagaagat tacgcgtact ctcagcagat gcagcgcgat
    24361 gcccgtcagc aggcgtttgc cctgacggaa gtggtgcagc gtcgtgcgca ctttagctac
    24421 tctgactcgg cagaaatgct tagcggtaac agcgatctca acgaaaaact gcgtgaacgt
    24481 ctggaacagg cggaagcgga gcgtacccgc gctcgcgaag cgttgcgcgg tcacgcagcg
    24541 cagttgagtc agtacaacca ggtgctggct tcgctgaaaa gttcttacga caccaaaaaa
    24601 gagctactca acgatctgca acgtgaattg caggatatcg gcgtgcgtgc tgatagcggg
    24661 gcagaagagc gggcacgtat tcgccgtgac gagctgcatg cacaactgag caataaccgt
    24721 tcgcgccgca atcaactgga aaaagcgctc accttctgcg aagcggagat ggataacctg
    24781 acccgcaaac tgcgcaagct ggagcgtgat tactttgaga tgcgcgagca ggtagtgacc
    24841 gcgaaagcgg gctggtgtgc agtcatgcgc atggtgaaag ataacggcgt tgagcgccgc
    24901 ttacaccgtc gtgagctggc ttatctctcc gctgatgatt tgcgttccat gtcggataag
    24961 gcgttaggtg cgctgcgtct ggcggtggcg gataacgaac atctgcgcga cgtgctgcgc
    25021 atgtcggaag atcccaaacg tccggagcgt aaaattcagt tcttcgtggc ggtttatcag
    25081 catctgcgtg aacgtattcg tcaggatatt attcgtaccg atgatccggt ggaagctatc
    25141 gaacagatgg agatcgaact tagccgtctg accgaagagt taacctcccg tgaacagaaa
    25201 ctggcgatca gttcccgcag cgtggcgaac atcattcgca aaaccattca gcgcgagcag
    25261 aaccgtatcc gtatgctcaa ccaggggttg cagaacgtat cgtttggtca ggtgaacagc
    25321 gtgcgcctca acgtgaacgt gcgtgaaacg cacgccatgc tgctggatgt gctctctgaa
    25381 cagcatgagc agcatcagga tctgtttaac agcaaccgtc tgaccttctc ggaagcgctg
    25441 gcgaaattgt atcagcgtct taatccgcag attgatatgg ggcagcgcac gccgcagacc
    25501 attggcgaag aactgctgga ttaccgcaac tatctggaaa tggaagttga ggttaaccgt
    25561 ggttccgatg gctggttgcg cgcagagtct ggtgcattgt cgaccggtga ggcgattggt
    25621 accggtatgt cgattctggt gatggtggta caaagctggg aagatgaatc tcgccgcctg
    25681 cgcggtaaag atatctctcc ttgccgcctg ctgttcctcg atgaagcagc gcgactggat
    25741 gctcgttcta tcgccacgct gtttgaattg tgtgagcgtt tgcaaatgca gctcatcatc
    25801 gcagcgccgg aaaatatcag cccggagaaa ggcaccacct ataaactggt gcgtaaagtc
    25861 ttccagaata ccgaacatgt tcatgtcgtc ggcctgcgag gatttgcgcc gcaactccct
    25921 gaaacgcttc caggaagtga cgaagcgcct tctcaggcga gttaaaatta aggcggcagc
    25981 aatgccgcct tttctttttc cgaaaactcc gtttctgcac taaaaaagtg gcacattacg
    26041 gcgcgctttt ctttaaactt ctttacatta ggttatgtaa aaacgtatcg gcgtttatat
    26101 actgaagata agcctgatga gtaacaggct tgctcgtcat actttcgtga gtattggcgt
    26161 tgtacaggca agtcgtaaaa taacagcctg gctattcaga gtatgataaa aacagggggc
    26221 aagggatgtt gcttaatatg atgtgtggtc gtcggctgtc ggcaatcagt ttgtgcctgg
    26281 ccgtaacatt cgctccactg ttcaatgcgc aggccgatga gcctgaagta atccctggcg
    26341 acagcccggt ggctgtcagt gaacagggcg aggcactgcc gcaggcgcaa gccacggcaa
    26401 taatggcggg gatcctgcca ttgcctgaag gtgcggcaga aaaagcccgc acgcaaatcg
    26461 aatctcaatt acccgcaggt tacaagccgg tttatcttaa ccagcttcaa ctgttgtatg
    26521 ccgcacgcga tatgcaaccc atgtgggaaa accgtgatgc tgttaaagcc ttccagcaac
    26581 agctggcaga ggtggcgatt gccggtttcc agccgcagtt taataaatgg gtagagttac
    26641 tgaccgatcc tggtgttaac gggatggcac gcgacgtggt gctctctgat gcgatgatgg
    26701 gctatctcca tttcattgca aatattccgg tcaaaggcac tcgctggcta tatagcagta
    26761 aaccttatgc gcttgcaacg ccgccgcttt cggtgattaa ccaatggcag caggcgctgg
    26821 ataaaggtca attgcctacg tttgttgcag gactggcacc gcagcatccg caatatgcgg
    26881 tgatgcatga atcgttactg gccttactca gtgacaccaa accgtggccc caactgaccg
    26941 gcaaagcaac gttgcgccca gggcagtgga gtaacgacgt accggcgttg cgtgaaatat
    27001 tgcaacgcac aggcatgttg gacggggggc cgaaaattac tctacctggc gatgacacgc
    27061 caactgacgc ggtagtcagc ccatccgctg ttactgttga aacagcagaa actaagccga
    27121 tggataagca aacgacgtct cgtagtaaac ctgcgcctgc cgttcgcgcc gcctacgata
    27181 atgaactggt ggaagccgtt aaacgttttc aggcatggca aggattgggg gcagatggtg
    27241 ctattggccc ggcaacgcgt gactggttaa acgtaacgcc cgcccagcgt gctggcgtgt
    27301 tggctctcaa catccagcga ttgcgcttgc tgccaacaga gctttctacc gggatcatgg
    27361 ttaacattcc ggcctattcg ctggtctact atcagaacgg caatcaggtg ctggattcgc
    27421 gagtcattgt cggtcgcccc gatcgcaaaa cgccgatgat gagcagtgcc cttaacaatg
    27481 tagtggtaaa cccgccgtgg aacgtacctc caactctggc acgcaaagat attctgccga
    27541 aagtgcgcaa cgatccggga tatctcgaaa gccatggcta tacggtgatg cgcggctgga
    27601 acagcagaga agcgattgac ccatggcagg ttgactggtc tacaatcacg gcctcgaatt
    27661 taccgtttcg cttccaacag gctccaggcc cacggaactc gctggggcgc tataaattca
    27721 atatgccgag ttcagaggcc atttatttgc atgacacgcc gaaccacaat ctgttcaagc
    27781 gtgatacacg cgcattgagc tcaggctgtg tacgagtgaa taaagcttcc gatctggcga
    27841 atatgctgtt gcaggatgca ggctggaatg acaaacgtat ttctgatgcg ctgaagcagg
    27901 gtgatacacg ttacgtcaat attcggcagt cgattccggt gaatctctac tacctgacgg
    27961 cctttgttgg tgcagatggt cgtacccagt atcgtacaga tatttacaat tatgatctgc
    28021 ctgcgcgatc cagctcgcaa atcgtatcga aagcggaaca attaatcagg taaatgaagt
    28081 agttcgggga aatcagttgt cgtaataatt agcatgattg ggggcaattc tctgcagccc
    28141 ccgtcactgc tggggttgag tcatcttgac gtctgcttta cgggcggtta aggtgcctct
    28201 tgtgcgccag aagtgcatat aaacgataac attgacctgt agacttgatt atcatggaca
    28261 aattcgacgc taatcgccgc aaattgctgg cgcttggtgg cgttgcactc ggtgccgcca
    28321 tcctgccgac ccctgcgttt gcaacactct ctaccccacg cccgcgcatt ttgacactca
    28381 ataatcttca taccggagag tcaatcaaag cggagttttt cgatggcaga ggctatattc
    28441 aggaagaatt ggcaaaactt aaccattttt tccgcgatta ccgcgcgaac aaaataaagt
    28501 ccatcgaccc aggattattc gaccagttgt atcgcctgca agggttatta ggcacccgca
    28561 aaccggtgca actcatttcc ggttatcgtt ccattgatac caacaatgaa ctacgcgccc
    28621 gcagccgtgg agtagcgaag aaaagctatc acactaaagg ccaggcgatg gatttccata
    28681 ttgaaggtat cgcgttaagc aatattcgca aagccgcgtt atctatgcgc gcaggtggtg
    28741 taggatatta cccacgtagt aactttgtgc atattgatac cgggccagca cggcactggt
    28801 agtaattgct taacgaaaca ggggcagtat gaactatcgt attattccgg tcaccgcatt
    28861 ctcccagaac tgttcattga tctggtgtga acaaacccgc ctggccgcac tggtcgatcc
    28921 tggcggcgat gcggaaaaaa tcaaacagga agttgacgcc agcggcctga cactgatgca
    28981 gatcctgctg acgcatggtc atctggacca cgttggcgca gcggcggaac tggcgcaaca
    29041 ttacggcgtg ccggtttttg gcccggaaaa agaagatgag ttctggctgc aaggcttgcc
    29101 tgcgcaaagt cgtatgtttg gtctggaaga gtgccagccg ctgacgccag atcgttggct
    29161 gaacgaaggc gataccatca gcatagggaa tgtgacttta caggtgttac attgccctgg
    29221 gcatacgccg ggtcatgtcg tgttttttga tgatcgggca aagctgctga tttctggcga
    29281 tgttattttc aaaggcggag tagggcgcag tgacttcccg cgtggcgatc ataatcaact
    29341 gatttcttca atcaaagata aattgctgcc actgggtgat gacgtgacat ttattccggg
    29401 tcacggacca ttatccacac ttggttatga acgcctgcat aatcccttcc tgcaagacga
    29461 aatgcccgtc tggtaaggca cacataaaaa agcccgcttt tcagcgggct tcattgtttt
    29521 taacgcttac agcactgcca caatcgcttc gcacagcgga gccatgttat ctggtgtcat
    29581 cccggccacg tttacgcgac cagaagcaac agcatacacg ccaaactctt cgcgcagacg
    29641 cagcacttgt tcttttgtca ggccactgaa ggagaacatg ccgttctgtt tgatgataaa
    29701 gctgaagtcg cggtttgcgc ctttttcctg cagcgtattg acgaacaact gacgcatacg
    29761 ctgaatacgc tggcgcatat cagtcagctc ttgttcccaa atcgcacgta acgcatcgtt
    29821 gctcaggatg gtggcaacaa cagaagcgcc gtgtgctggt gggttagagt agttagcgcg
    29881 aatcgccgct ttcatttggc tgaatgcgcg atcaacggtt tcactgtcgg cagcaaccag
    29941 agtacaagcg ccaacacgct cgttgtacag gccaaagttt ttagagtagg aactggcaac
    30001 aatcagctct ttatgcatag ccgcgaaagc gcgcagtcct tcagcatctt cttccagacc
    30061 acgggcaaaa ccctggtaag cgaagtcaaa cagcggtaac cagcctttct caacggagag
    30121 ttgtgccagt gtctgccatt gttccagcgt agggtcgata ccggttgggt tgtggcagca
    30181 gccatggaac agcactacgt cgccagcctg agcttcgttc aggctgttaa tcagtgcatc
    30241 gaagtcaagg gtgtggtttt ccgcatcata ataagcgtat tcacgaactt ccagatctgc
    30301 agagttaaag acgctcttat ggttcggcca gcttgggttg ctcacccaca ctcgcttaac
    30361 gctggtattt tttgccagga aatcggcagc tatgcgtagt gcgccagtgc cacccggagt
    30421 ctgtgccgtg cgagcacgtt tgtcattgat cagggcgcta cctttaccaa acagcagttc
    30481 ctgagtgcag cgaccaaatt cagggatgcc gtcaatgccg aggtaatttt tggtggtttc
    30541 attttcgagc agatactgtt cagccttttt cacgctggtc agtaccgggg ttttgcccgt
    30601 ctcatcttta tagacaccaa tcccgaggtt aattttgccg ggacgttcat cggcacgaaa
    30661 cagatcggcc aggcccagaa tcgggtcggc aggagcggcg gtaatgttct caaacatgac
    30721 gaggttccat tatggttaca gaagggaagt ccgctatcag ggtaacggga gatttacaaa
    30781 attccaacta ttactgatga aaacgcaggc tgtttttgca agacgtgaga ttgctctgga
    30841 aggcataaaa aaaacaggac caaagtcctg ttttttcggc atttaacaaa gaggtgtgct
    30901 attagaactg gtaaacgata cccacagcaa cggtgtcgtc tgaacctacg cccagtttgt
    30961 tgtcagaatc gatctggttg atgatgtagt caacataggt ggacatgttt ttgttgaagt
    31021 agtaggttgc gcccacttca aagtagttca ccagatcaac atcaccgata ccttctacgt
    31081 ctttcgcttt agatttggtg taagcgatgg acggacgcag accgaaatcg aactggtatt
    31141 gcgcaactaa cagaacgtct tgcgttttgt tggcgaagcc gctgatgttt gtaaatttat
    31201 tagtgatcgg cgtagcgtta cgcgtttcac cgtagttcgc tgccaggtag atgttgttcg
    31261 catcatactt cagaccagta gcccactgtt cagctttttt accgttgcca agaagttgag
    31321 cttcttgcag gttggtacgg tcagctgcac cataagcacc aacgatacca aagccttcgt
    31381 attcgtagct gatagaaccg ccaacaccgt cgccgttaga gcggcgtgca gtgtcacgct
    31441 cgtttttacc caggtactga acagcgaagt tcaggccatc aaccagacca aagaagttgg
    31501 agttacgata ggtagcaacg ccgccaacac gaccaacgaa gaagtcatcg ctgtatgcag
    31561 tatcaccacc aaattctggc agcatatcgg tgtaacccag tgcatcataa accacaccgt
    31621 agttacggcc gtaatcgaaa gaaccaacgt cagcgtattt aagacccgcg aatgccagac
    31681 gcgttttgtt accagtttga gcgtcagcgc cttcagagtt gttaccctgg aagttatatt
    31741 cccactgacc ataaccggtc agatcggaat tgatttgagt ttccccttta aaaccaagac
    31801 gggcataggt catgtcgcca ttgccaccgt aactgttttc accgttaccc ttggagaaat
    31861 aatgcagacc gacagcttta ccgtacagat ctactttgtt gccatctttg ttatagattt
    31921 ctgcagcgtt tgcagtacct gctactaaca gagcagggac gatcactgcc agaatattgc
    31981 gcttcatcat tatttattac cctcatggtt ttttttatga cacctgccac tgccgtcaat
    32041 aatttctgtc aataaaattt tacggaacta ttgatgagag tttggtgtct ttatgtgtct
    32101 gcaggcatct ttccattcaa actaacgttt cgctaccgtg aaagtgctac aaagataaag
    32161 atttagtttc aaaaagaaaa aatatgtaac taaaagtaaa a
//
LOCUS       DBFIFM010000033        32124 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.033, whole genome shotgun sequence.
ACCESSION   DBFIFM010000033 DBFIFM010000000
VERSION     DBFIFM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.033"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(69..887)
                     /gene="cof"
                     /locus_tag="N7C74_001353"
     CDS             complement(69..887)
                     /gene="cof"
                     /locus_tag="N7C74_001353"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HGU2431251.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGAISLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLHEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            987..2687
                     /gene="ybaE"
                     /locus_tag="N7C74_001354"
     CDS             987..2687
                     /gene="ybaE"
                     /locus_tag="N7C74_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HGU2431252.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEKALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSADGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMTDATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            2752..3447
                     /gene="queC"
                     /locus_tag="N7C74_001355"
     CDS             2752..3447
                     /gene="queC"
                     /locus_tag="N7C74_001355"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HGU2431253.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKVLNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(3499..3897)
                     /gene="fadM"
                     /locus_tag="N7C74_001356"
     CDS             complement(3499..3897)
                     /gene="fadM"
                     /locus_tag="N7C74_001356"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HGU2431254.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(4003..4374)
                     /gene="ybaV"
                     /locus_tag="N7C74_001357"
     CDS             complement(4003..4374)
                     /gene="ybaV"
                     /locus_tag="N7C74_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HGU2431255.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QNKAAVPAKASDEEGSRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(4525..6396)
                     /gene="ppiD"
                     /locus_tag="N7C74_001358"
     CDS             complement(4525..6396)
                     /gene="ppiD"
                     /locus_tag="N7C74_001358"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGU2431256.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADLQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDVAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKENLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(6588..6878)
                     /gene="hupB"
                     /locus_tag="N7C74_001359"
     CDS             complement(6588..6878)
                     /gene="hupB"
                     /locus_tag="N7C74_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HGU2431257.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(7069..9423)
                     /gene="lon"
                     /locus_tag="N7C74_001360"
     CDS             complement(7069..9423)
                     /gene="lon"
                     /locus_tag="N7C74_001360"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HGU2431258.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILNTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(9611..10885)
                     /gene="clpX"
                     /locus_tag="N7C74_001361"
     CDS             complement(9611..10885)
                     /gene="clpX"
                     /locus_tag="N7C74_001361"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HGU2431259.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(11011..11634)
                     /gene="clpP"
                     /locus_tag="N7C74_001362"
     CDS             complement(11011..11634)
                     /gene="clpP"
                     /locus_tag="N7C74_001362"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HGU2431260.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(11879..13177)
                     /gene="tig"
                     /locus_tag="N7C74_001363"
     CDS             complement(11879..13177)
                     /gene="tig"
                     /locus_tag="N7C74_001363"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGU2431261.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(13521..13838)
                     /gene="bolA"
                     /locus_tag="N7C74_001364"
     CDS             complement(13521..13838)
                     /gene="bolA"
                     /locus_tag="N7C74_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HGU2431262.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            14143..14721
                     /gene="yajG"
                     /locus_tag="N7C74_001365"
     CDS             14143..14721
                     /gene="yajG"
                     /locus_tag="N7C74_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU2431263.1"
                     /translation="MFKKILFPLVALFMLAGCARPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            14765..16240
                     /gene="ampG"
                     /locus_tag="N7C74_001366"
     CDS             14765..16240
                     /gene="ampG"
                     /locus_tag="N7C74_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HGU2431264.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTAYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            16700..17647
                     /gene="cyoA"
                     /locus_tag="N7C74_001367"
     CDS             16700..17647
                     /gene="cyoA"
                     /locus_tag="N7C74_001367"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HGU2431265.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGFATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPNLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            17669..19660
                     /gene="cyoB"
                     /locus_tag="N7C74_001368"
     CDS             17669..19660
                     /gene="cyoB"
                     /locus_tag="N7C74_001368"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HGU2431266.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            19650..20264
                     /gene="cyoC"
                     /locus_tag="N7C74_001369"
     CDS             19650..20264
                     /gene="cyoC"
                     /locus_tag="N7C74_001369"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HGU2431267.1"
                     /translation="MATDTLTHATVHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            20264..20593
                     /gene="cyoD"
                     /locus_tag="N7C74_001370"
     CDS             20264..20593
                     /gene="cyoD"
                     /locus_tag="N7C74_001370"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HGU2431268.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            20605..21495
                     /gene="cyoE"
                     /locus_tag="N7C74_001371"
     CDS             20605..21495
                     /gene="cyoE"
                     /locus_tag="N7C74_001371"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HGU2431269.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            21644..23008
                     /gene="yajR"
                     /locus_tag="N7C74_001372"
     CDS             21644..23008
                     /gene="yajR"
                     /locus_tag="N7C74_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2431270.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLRNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IRQA"
     gene            complement(23136..23627)
                     /gene="yajQ"
                     /locus_tag="N7C74_001373"
     CDS             complement(23136..23627)
                     /gene="yajQ"
                     /locus_tag="N7C74_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HGU2431271.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            23795..24706
                     /gene="panE"
                     /locus_tag="N7C74_001374"
     CDS             23795..24706
                     /gene="panE"
                     /locus_tag="N7C74_001374"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HGU2431272.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAACRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRTLRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            24669..25259
                     /gene="yajL"
                     /locus_tag="N7C74_001375"
     CDS             24669..25259
                     /gene="yajL"
                     /locus_tag="N7C74_001375"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HGU2431273.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLIRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(25313..26761)
                     /gene="thiI"
                     /locus_tag="N7C74_001376"
     CDS             complement(25313..26761)
                     /gene="thiI"
                     /locus_tag="N7C74_001376"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HGU2431274.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     DGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTG
                     QEVVEVETVNDFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFKNVKVYRP"
     gene            26967..27209
                     /gene="xseB"
                     /locus_tag="N7C74_001377"
     CDS             26967..27209
                     /gene="xseB"
                     /locus_tag="N7C74_001377"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HGU2431275.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            27209..28108
                     /gene="ispA"
                     /locus_tag="N7C74_001378"
     CDS             27209..28108
                     /gene="ispA"
                     /locus_tag="N7C74_001378"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HGU2431276.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKAQDLIDDARQSLKQ
                     LAEQSIDTSALEALADYIIQRNK"
     gene            28133..29995
                     /gene="dxs"
                     /locus_tag="N7C74_001379"
     CDS             28133..29995
                     /gene="dxs"
                     /locus_tag="N7C74_001379"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HGU2431277.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDETLILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            30050..31024
                     /gene="yajO"
                     /locus_tag="N7C74_001380"
     CDS             30050..31024
                     /gene="yajO"
                     /locus_tag="N7C74_001380"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HGU2431278.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFSRREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            31255..>32124
                     /gene="espY3"
                     /locus_tag="N7C74_001381"
     CDS             31255..>32124
                     /gene="espY3"
                     /locus_tag="N7C74_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspY3"
                     /protein_id="HGU2431279.1"
                     /translation="MMKTITKQPILFTDVPVADLRNSMKQDLNQNLIERLWNKIRDFF
                     LDSDKQKAFKSIHKYINTLSVLNYNSALTPDPNFNIDATSDLDSYLKLDFDRLSPKQK
                     QTTLCCFWNKIASSLPEPYNSTIKHNIIFYKDGENLMIRGTISIVNEVVKTYSLPIEK
                     DDNGYYDFSGLYLAHSNISGKDPNKDPDIDFGIDMGNCNCSNVNFEHTYFYGVKFTNA
                     NCTNANFNNCRFKKCDLTNMNCTGAILDNAMIYGKEKEPEMQYPEADQIIQRITYQKS
                     DGNETKGMILTNCS"
BASE COUNT         7509 a         8382 c         7983 g         8250 t
ORIGIN      
        1 ccacggcaca aaatgacaaa ttcaacgcaa aaaaaccggg caattgcccg gtgctggaag
       61 ggatctcgtt attcggggga ataaggtaga tgtggatagt ccagccagtg cgtcaaatag
      121 tgagagacag cctgatttcg gcaatgtcca atcaccggta aatgcgggag ctccgcgcgc
      181 agttgcggca tcgcattgcc cataataaat ccgctaccga cgctgcctaa catttcgcga
      241 tcgttcatcg catcaccaaa ggccatgcaa tcgcgcaacg ataaacctaa atgttgggtc
      301 agcaccgtca gtgcagcgcc tttattgcag cccaccggca gcacttcaag gcaatccgtg
      361 gcggaaaaac acaaatgtgc acgctcgcct aatgcttcgt gtagctggat ctgcaaacgt
      421 gtaagatcgt cgtgatcgcc acagaagcag atcttggtga cgctgccgag tggcattttt
      481 ttgacatcga ttatttgata acgaaaaccg ctatagacaa atgcctgcaa caacgcaggg
      541 atctctttcc cggtaaacca accgtcgtca ttgaaaatat gcatgctggc tcgggtatcc
      601 cattgctgat acagcaccag ctccgcgaca tccgcaggta aatcatcacg atgtaaaagt
      661 tcaccttcca gagaatgcac gcgcgttccg ttgccggtaa tcaaatacgc atccagcgat
      721 atcgccccga gaatatgctg catctccagc gcatgacgcc ccgtggcaaa agtgagggta
      781 atgtcgcgtt cacgcagtcg cgccaaagta gagagggttt tctcacctaa atgatggtca
      841 ggcatcaata aagtgccatc catatcaaat gctgccagac gagccatttc tttctccact
      901 ctgtgacacg gttaatggtg attgagtatc acctgatata tacggaagta atagtgaata
      961 gttaaataat attgttccgg gtttttatgc gactgctcaa ccgccttaac cagtatcaac
     1021 gtctgtggca accttccgcc ggaaagccgc aaaccgtcac cgtcagcgaa ctggccgaac
     1081 gctgtttttg cagcgaacgc catgttcgta cgctgttgcg tcaggcacag gaagcgggat
     1141 ggctggagtg gcaggcgcag tcaggacgcg gaaagcgcgg acaattacgc tttctggtca
     1201 cgccagaatc gctacgcaat gcgatgatgg aaaaggcact ggaaaccgga aagcagcaag
     1261 atgtgctgga gctggcgcaa ctggccccag gtgagctgcg cactctgtta cagccgttta
     1321 tgggcggaca atggcaaaac gatacaccca cgttgcgtat tccctactat cgcccgctcg
     1381 aaccgctaca accgggcttt ttgcccggcc gtgccgagca gcatctcgcc ggacagatat
     1441 tttccggcct gacccgcttc gataataata cccagcgccc gattggcgat ttagcgcatc
     1501 actgggaaac ctctgctgac gggttacgct gggactttta tcttcgttca accctacact
     1561 ggcataacgg cgatgcagta aaagcctcac acttacacca gcgattattg atgctgttac
     1621 aactgccagc actggatcaa ttatttatta gcgtgaagcg tattgaggtc acccatccgc
     1681 agtgtctgac cttcttttta catcgccccg attactggct tgcgcaccgg ctggcgagct
     1741 attgcagcca tctggcgcat ccgcaattcc cactgattgg cacgggtcct tttcgcttaa
     1801 cacaattcac agctgaactg gtgcgcctgg aaagccatga ttattaccat ttacgtcatc
     1861 cgctgcttaa agcggttgag tactggataa ctccgccgct tttcgaaaaa gatttgggaa
     1921 ccagttgtcg gcatcccgtg caaatcacca tcggcaaacc ggaggagctg caacgggtca
     1981 gccaggtcag tagtggcatc agtttaggtt tttgctattt aacgttgcgc aaaagtcccc
     2041 gactctccct ctggcaggcg cgaaaagtga tctccattat tcatcaatcc ggtttattac
     2101 aaacgttaga agtcggagaa aacctgatca ccgccagtca tgcattactg ccaggctgga
     2161 ctattccgca ttggcaagta ccggatgaag tcaaactacc gaaaaccttg acgctggttt
     2221 atcacctccc gatagaactt cataccatgg cagaacgcct acaggcgaca ctggcagcgg
     2281 aaggctgtga actcacaatt atttttcata acgcaaaaaa ctgggacgac acgaccctac
     2341 tggcacacgc agacctcatg atgggcgaca gattaattgg cgaagcaccg gaatatactc
     2401 tggagcaatg gctgcgctgc gatccgctgt ggccacatgt tttcgacgct ccagcatacg
     2461 cacatctaca atcgacactg gacgcggtgc aagtaatgcc tgatgaggaa aaccgattta
     2521 atgccctgaa agcggttttt agccagttaa tgacagatgc gacgctgacg ccgctgttca
     2581 actatcacta tcgcattagt gcccctcccg gcgtgaacgg tgtgcgactg acaccgcgcg
     2641 gctggtttga atttaccgaa gcctggcttc ccgcgccgtc gcaatgaata gctggtccgg
     2701 gttaatcgca ggcgttacca taacgctttt attgttcaag caggattatc tatgaaacgt
     2761 gctgtcgttg tgttcagtgg aggccaggat tccaccacct gtctggtgca ggcattacaa
     2821 caatatgatg aagtccattg cgtgacgttc gattacggtc agcgccatcg cgcagaaatc
     2881 gacgtggcac gcgaactggc gctgaaactg ggggcacgcg cgcataaggt tcttgatgta
     2941 acgctgctca acgagctggc ggtcagcagc ctgacgcgtg acagcattcc ggtgcctgat
     3001 tatgaacctg aagccgatgg catcccgaat acgtttgtcc cagggcgtaa tattttgttc
     3061 ctgacgctgg cggcaatata tgcgtatcag gtaaaagcag aagccgtaat taccggcgtc
     3121 tgcgaaacgg atttctccgg ctacccggat tgccgcgatg agtttgtgaa agtactaaac
     3181 catgccgtca gtttgggcat ggcgaaagat attcgttttg aaacgccgct gatgtggatt
     3241 gataaagcgg aaacctgggc gctggcagat tattacggca aactggattt agtccgtaac
     3301 gaaacgttga cctgctataa cggcattaaa ggcgacggtt gcggtcattg tgcggcatgt
     3361 aatttacgcg ccaacggttt gaatcattat ctggccgata aaccgacggt gatggcagcg
     3421 atgaagcaga aaaccgggtt gaagtaatta ttccgggtgt cgccggatgc ggcttgagca
     3481 tccggcacca caaaacgttt acttaaccat ctgctccagc ttttcgcgca attccccttc
     3541 cagagctaat gctttctgcg ttttaagatc aatacaaaca aacgtaataa gcgcatccgc
     3601 taccacctgc ccttccggct ccagtgtaat gacctggctt aagatgccgc ttttaccgtt
     3661 taattgctgt aactggctcg taatggttaa caagtcgctt aataccgccg gacgacgata
     3721 gttaatattg atattcacca ccacgaaagc gatgttatgg gccgtcatcc agtgaaaact
     3781 gtcgctattt tccaacccat cccagcgggc ttcttcgaga aattcaaggt agcgggcgtt
     3841 gtttacgtgc tggtaaacgt cgagatgata tccacgaact ttgatttgtg tttgcatagc
     3901 gcaataacct tacgttgttt ttataaaaac tgttataagt acagaggtca taactggtat
     3961 gacctcttca gtctggcaaa aaattgccac tatgcaaatt aattacaggg ttaataccgc
     4021 cagattacgt tccaccagcg aattgcccat ccccggcacc tgctttagat cctccaccgt
     4081 tttaaacgga ccgtactctt cgcgataact gacaatcgct tgcgctttct tcaggccaac
     4141 gccattcatc gcgcgggcta gctcttccgc gctggcatta ttaatgctga cccgggagcc
     4201 ttcttcgtca ctggctttcg ccggtactgc tgctttattt tgtgctgcag gagcttccgc
     4261 tttggtttct accgccgtcg gtttcgccac agaagctgcc gccagcgcgc tatgagacat
     4321 tccggcacag gccagggaca gggtaatgag cagtgcttta attccgtgtt tcatactgtt
     4381 ttctccttgt ttgttaacag tggaatcacc ttaacggcag tgaaaacagt aaacaaatgg
     4441 caatgttcag aaatggaaaa ggccgcgaaa gcgacctttt cttgttacag ctgcaaagca
     4501 tttttttgcg aggcggcttc aggattattg ctgttccaac gcatcgccaa ttttgatttt
     4561 cgcctcttta cgcaggttac tcatcagagc ttcaaagacg atttgtgcgt tgttctgggt
     4621 gataccctgc accatcgctt ttttctgatc ttccggcatt gaaccttgtt tcacttcatc
     4681 cagcgccagc agaaccacat taccttgcat atcggtcgcc ataccgtagc tcggtttgtc
     4741 tttcgctggc agtggcagtg caaacgccgc ctggctaatc gggtcacgac cggaacggct
     4801 taaggttttc ggctcgccaa atttcagacc ggcagcctgc atagcttccg cacctttgcc
     4861 ggctttcaaa tcaaccagca gtttctcagc atccactttc gcctgttgtt cagctttgtt
     4921 gtgctgaacc agtgccttaa cttgttcctg aacatctgcc aacggtttca ccgcttccgg
     4981 tttgtgctcg ctgatgcgca gcacgaatgc gcggtcgccg tctacggtga tgatgtcaga
     5041 gttgatgccc ggcgcgccgt tttcacctac cagaccgccg ttaaagatag cgtcagcaac
     5101 cggcttgaag ttcaactctt ccggcaggtt ctctttgctg aaccaacccg tctgagtggc
     5161 tttaacgccg gcagcttgct ctgcaccggc cagagactcg gtgtcgttgc ttgccgcatc
     5221 gctcactttc tgctgcagcg cgtagtacgc atcgagggct ttttcgtgtt tcactttcgc
     5281 cgcgacatcg tcacgtactt cgtctaacga tttcactttc gctggctgaa tgtcgtccag
     5341 acgtacaatc aggaaaccga ccgaagattt gatgacacca gacagttggc ctttttcttt
     5401 cagaccagca tttttcagtt catccgggat agtggcatct tctaaccaac ccatatcgcc
     5461 gccgttacga gcagagataa tatcggcaga tttttctttg gctaatgcag caaaatcacc
     5521 gcctttattc agctcatcaa gtaccgcttt cgcttcatct tcagttttgg tctggatgat
     5581 gctgtaacgg gtacgctgcg gctgggtgaa ttgatcctga tgctgatcgt agtagctctg
     5641 gagatccgca tcgctaaccg gttgctgcat cgttgcggca tccagcttga tgtaactcac
     5701 gcggaattgt tccggcgtca tgaaattgtt tttgttttgt tcgtagtagc tggcaatttc
     5761 ctgttcggtc acaggctgct tcgccgccag cgcgttaaca tcgatagtcg cttcacgcac
     5821 cacgcgttgt tgagcgacca gtgccgccag ctcgtcggtt tcacctttca gcataaaatc
     5881 ggtaccagca acgccgttaa tcagctgttg ggtagtgagc tggttacgca gcgcctgggc
     5941 gtactgatcg gcggtcatcc ccatctggtt gaggataccg ttatagcggc tgttatcaaa
     6001 tttgccatca acctggaagg ctggggtcgc gaaaatcgcc tgtttaacct gctcatcgct
     6061 gatacccagt ttcagctcac gtgcgtactg atccagcagc gcctcgtcga tcagacgatt
     6121 cagcacctgt tgacgcaggg ttttcatata gccttcgttc gctgccagct cggagtattg
     6181 atcgcccagc tgttgctgca tgcgattacg ctcgctgttg aaggcattct cgaattgccc
     6241 acggctgatt tcctggtcat tcacttttgc ggcgtagtta ttgcctccgc caatcaggta
     6301 accactcacg ccggtcaata tgaacgacac gataatgata ccgaaaataa tcttgagcac
     6361 gagactgttt gcagccgtgc gtaagctgtc catcatggtg taacaacact ccgctgtaga
     6421 tgactgtgta cctcacgcaa cgtatcgatg cgcgcaaagg cctattgtga caagaaacgg
     6481 gggcaattgt cagcccacaa gacgcagaaa gtcaccgaat acaaataaaa aaggcacatc
     6541 agtagatgcg cccttgaact tcgtcacatc cccactgggg acaacgctta gtttaccgcg
     6601 tctttcagtg ctttacctgc acggaagctc ggtactttag cggcagcgat ggtgatctct
     6661 ttaccggtct gcgggttgcg gccagtacgg gcagcacgct ctttaacggc aaaagtacca
     6721 aaacctacca gtgctacatc atccccttct ttcagagatt cagttacgga agcaataata
     6781 gcatctaacg cacggccagc cgcagcttta gagatatcag cccctgcagc aatcttgtcg
     6841 atcaattgag atttattcac tcttctcttc ctctttataa tttatatcgc acttgaatcc
     6901 ttcaaggtac gaacgcgcag cagttatatc aggccagcca tccccttaca acacccctta
     6961 ataagggcaa gcccgatccg ccatctaact tagcgagaca aaaaaaggct ggcaagcccg
     7021 aattagcctg ccagccctgt ttttattagt gcattttgcg cgaggtcact attttgcagt
     7081 cacaacctgc atgccagacg gttcattttg cagcgccaga gtcagaactt cctcaatgcg
     7141 cttcacagga tgaatgtcca gatcggcaat tacgttgtca ggaatctctt ccagatcgcg
     7201 tttattttcg aacggaatta acactgtttt aatcccgccg cgatgcgctg ccagtagttt
     7261 ttctttcaaa ccaccgatcg gcagtacctg accacgcaga gtgatctcac cggtcattgc
     7321 cacatcggca cgaaccgggt taccggtcag gcaagaaacc agcgcggtgc acatagcaat
     7381 accggcactc ggaccatctt tcggcgtcgc accttccggt acgtggacgt ggatgtcacg
     7441 tttttcgtaa aaatcagggt tgatccccag tttttccgca cgcgcgcgaa ccaccgttaa
     7501 cgcagcctga atagactcct gcatcacttc gccgagcgaa ccggtatagg tcagtttgcc
     7561 tttacccgga acacatgcgg tttcaatggt cagcaagtca ccgcccactt ccgtccacgc
     7621 cagaccggtt acctgaccga cacggttttc gttatcagcg cgaccatagt cgaaacgctg
     7681 aacaccaagg tagtcatgca ggttgtcgcc gttaatttcg atatgtttta atgacttatc
     7741 gagcagtaac tgcttaaccg ctttacggca cagcttggag atttcacgct ccagaccacg
     7801 cacgcccgcc tcacgggtgt agtaacgaat aatgccgata atggcgctat cgtcgacggt
     7861 cagctcacct tttttcagtg cattacgttc aatctgcttc ggcagcaggt gacgtttggc
     7921 gatgttcagt ttttcatctt cggtataacc ggagaggcga atcacttcca tacgatccag
     7981 cagtggtgcc ggaatgttca tggagttcga cgtcgcgaca aacatcacgt cgctgagatc
     8041 gtaatccact tccaggtagt gatcgctgaa ggccacgttc tgctctggat ccagcacttc
     8101 aagcagtgcg gaagccggat cgccacgcat gtcagaagac attttgtcga tctcatcgag
     8161 caggaacagc gggtttttaa cgcccacttt cgccattttc tggatcaatt tacccggcat
     8221 agaaccgatg taagtacggc ggtgaccacg gatttccgct tcatcacgca cgccgcccag
     8281 cgccatacgg acatatttac gcccggtggc tttggcaatg gactgaccca gggaggtttt
     8341 acctaccccc ggcggcccta ccaggcaaag gatcggtccc ttgattttgt tgacacggct
     8401 ttgaaccgca agatactcaa ggatgcgatc tttcacgcgc tccagaccat aatggtcggt
     8461 attaaggatt tcctgcgcct gacgcaggtc ttttttgacc ttgctgcgcg cattccacgg
     8521 tacctgtacc atccagtcga tataaccacg cactacggtc gcttctgccg acatcggaga
     8581 catcattttc agcttctgca actctgcttc cgctttctct tttgcctctt tcggcatctt
     8641 cgccgcgtcg attttgcgct tcagggcttc gttttcgtcc ggcgcgtcgt ccatctcacc
     8701 gagttctttc tgaatagctt tcatttgctc gttcagatag tactcacgct gggatttctc
     8761 catctgcttt ttaacgcggt tgcgaatgcg tttctcaacc tgcagcagat cgatttccga
     8821 ttccatcatt gccatcagat attccagacg ttcgttaacg tcggacatct ccagaacgga
     8881 ctgtttgtca gccagtttca gcggcatatg tgcagcaatg gtatccgcca gacgcgccgg
     8941 atcgtcgatg ctattcaacg acgtcagcac ttctggtggg atttttttgt tcagcttgat
     9001 gtagccttcg aactggctga ttgcagtacg caccagcact tcctgttctc gctcatcaat
     9061 ggtcggcgac tccagatact ccgccttcgc agaaaagtgt tcgccattgt cagagagcgc
     9121 agaaatacgc gcgcgctgta acccctcgac cagcactttg acggtgccgt caggcagttt
     9181 cagcatctgc aatatagagg ccacggtccc gacggtgaaa agatcgttta cacccggctc
     9241 atccgttgaa gcttctttct gcgcgaccag cataattttt ttatcatggt ccatcgccgc
     9301 ttccagacaa cggatagatt tttcccgccc gacaaataag gggatgacca tgtgcggata
     9361 aaccaccaca tcgcgcagcg gcaatacggg gatttcaatg cgttcagaac gctcaggatt
     9421 catagagctc tctcttagtt taatttccgc caggtaatca gatgacacga ctgtgcttca
     9481 cgccatctat taacatgtac gtcagtatat ggggatgttt cccccacatt caacgccgag
     9541 aatagaggaa aaattaaagg ggagataaaa tccccccttt ttggttaact aattgtatgg
     9601 gaatggttaa ttattcacca gatgcctgtt gcgcttccgg cttgccataa atcagcaacg
     9661 gtttgctttg accatcaatt accgactcgt cgataaccac tttttcgaca tcttccatgg
     9721 acggcagatc gtacatggta tcgagcagtg cggcttctac gatggaacgc aggccacggg
     9781 caccggtttt acgcgccatc gctttcttag cgatagcatc cagcgcctcg tcacggaatt
     9841 ccagatccac gccttccaga ttaaacagcg cctgatactg cttggtcagg gcgtttttcg
     9901 gctctttgag gatctgaatc agagcttctt cgctcagttc attcaacgtt gcgacaaccg
     9961 gcagacgacc aataaactca gggataagac caaacttgat cagatcttcc ggttcaacct
    10021 gcgccagcag ctcgccttcg cttgctttgt cggacttcgc ttttaccgtc gcgccaaaac
    10081 caatgccgga gccggtttct acacgatggg aaatcacttt atccagaccg gcaaacgcac
    10141 cgccacaaat aaacaggatc ttagaggtat caacctgcaa gaattcctgc tgcggatgtt
    10201 tacgtccacc ttgcggtgga acagcagcta ccgtaccttc gatcagtttc aacagtgcct
    10261 gctgtacgcc ttcaccggaa acgtcacggg taatagacgg gttgtctgac ttacgagaaa
    10321 tcttgtcgat ttcatcgatg tagacaatcc cgcgctgcgc tttctgtacg tcgtaatcgc
    10381 acttttgcaa cagcttctga atgatgtttt caacgtcttc gcccacataa ccggcttcgg
    10441 tcagcgtggt tgcgtcggcc atggtgaacg ggacgtccag caggcgcgcc agcgtttcag
    10501 ccagcagcgt tttaccggaa ccggtcggac cgatcagcag aatgttactt ttgcccaact
    10561 cgacgccatt gctggtatcg ccgttgcgca gacgtttgta gtggttgtat accgcgaccg
    10621 ccagcacttt tttcgcctgt tcctggccga taacgtaatc gtccaggtgg ttacgaattt
    10681 catgcggcgt cggtagcgca ctgcgttcac gatgcggtgc aacttcttta atctcttcgc
    10741 gaatgatgtc gttacataaa tcaacacatt cgtcgcagat atacacggat ggaccggcaa
    10801 tcagcttgcg cacttcatgc tggcttttgc cgcaaaaaga gcaatacagc aatttgcctg
    10861 agccatcttt gcgtttatct gtcatgggtc aaaacctctt ctttgttctt tgtgccgcac
    10921 acgacgacgc aaatgccatt ctcaggcgca agccgcttac agcgttgtgc cgccctggat
    10981 aagtatagcg gcacagttgc gcctctggca tcaattacga tgggtcagaa tcgaatcgac
    11041 cagaccgtat tccaccgctt caggggcgga aaggaagcga tcgcgctcgg tatcacgttc
    11101 aatctgttct aatgattgac ccgtatgaag cgccataagt tcattcatac gccctttaac
    11161 tttcagaatt tcacgggcat gaatttcgat atcggtcgcc tggccctggt agccgcccaa
    11221 cggttggtga atcatcacgc gcgaattcgg caggcaaaaa cgtttacctt ttgcccctgc
    11281 ggtcagcaag aaagcgccca tcgaggccgc ctggcccata cagatggtgc tgacatcagg
    11341 cttgataaac tgcatggtgt catagataga catcccggca gtgatcaccc cgcctgggga
    11401 gttaatgtac agatagatat ctttttctgg gttttctgct tccaggaaca gcatctgagc
    11461 cacaatcagg ttagccatat ggtcttcaac ctggccagtc agaaaaatga cgcgttcctt
    11521 aagtagacga gaatagatat caaaagagcg ctcaccacgt gaggtctgtt caatgaccat
    11581 cggcaccagc gccatatggg gtgcaaagtt atctcgttcg ccgctgtatg acatttccgt
    11641 ctcctggata aaattgaaaa aacctgctgt accgattgta accttatgga cggattatca
    11701 gctagtccct gtgttatggg tacggcatca ccatatttca agcataacaa tcttttgttg
    11761 ttacgctaac accgaaagag gcttttcgca cggttccatg caaaattcat gacaaaaaaa
    11821 gcccaccacc tggcggtgac gggcctttgt gcgaatttag cgcgttatgc tgcgtaaatt
    11881 acgcctgctg gttcatcagc tcgttgaaag tggtttcttt ttcagtcact ttcgctttcg
    11941 ccagtacagc ttcaacagcc tgttcttcca gagcaacatt gcgcatgttg tccatcagtt
    12001 ctttgttttt gctgtagaac tcgataactt ctttcggatc ttcgtacgct gaagccatct
    12061 cttcgatcag gcctttcacg cgctcttcgt cagctttcag ctcgttggtg cggataactt
    12121 cgcccagcag caggccaaca actacgcggc gtttagcctg ttcttcgaac agttcgcgcg
    12181 gcagttccag agcttgtttt tcgttgccac cgaaacgctg tgcagcctgg cgacgcagaa
    12241 cgtcgatttc gctgtcaata agcgcagccg gtacatcgat gtcgttagct tttaccagac
    12301 cttcgatcgc ctgagactta acgcggttac ggatggcgct cttcagctcg cgctccatgt
    12361 ttttacgcac ttcagcgcgc agaccttcta cggaaccatc ttcaacgccg aaacgtttga
    12421 tgaattctgc agtcagttcc ggcagttcac gctcttcaac tttcttcagg ttgatagcga
    12481 atttcgctgc tttacctttc aggttttctg cgtggtattc ttccgggaag gtcacgtcga
    12541 tggtgaactc ttcgccagct ttgtggcctt tgataccgtc ttcaaagccc gggatcatac
    12601 gaccctggcc catcgccagt acgaaatcag acgctttacc gccttcgaac tcttcgccgt
    12661 ctacagaacc ggtgaagtcg atggttacgc ggtcttctgc ttcaacagcg ccgtcttttt
    12721 ctttccaggt cgcctgctgt ttacgcagag tatccagcat gccgtcaacg tcagcgtcgg
    12781 tcacttcaac gatcggtttt tcaacttcga tcgcttccag accctgcagt tcaacttccg
    12841 gataaacttc aaactctaca gagtaggtga agtcttcacc cagcttgtat tcgcccggaa
    12901 cataagtcgg tgcgccagcc ggattgattt tttctttaat gatggcgtca atgaagttac
    12961 ggctcatcag gtcacccaga acgtcctggc gtacagacgc gccataacgc tgagcaacga
    13021 tattcattgg cactttgcct ttgcggaagc cgtcaatacg tacttttttc gcaacgttga
    13081 ccagctcgct tttaacagcg gtctcgatgc tgtcagcagc gatagtaatc gttacacggc
    13141 ggccaaggcc ttgagtggtt tcaactgaaa cttgcatctt gttacctcaa aaaatcacag
    13201 tgctcggtca actcttaccc cgcaagcaca ggttgttggc ttcgcggaac cgggatgttc
    13261 ttataatcaa tcacattccc tgttaccaga atcatcccga aagcattcaa ataggacgcg
    13321 gcattatagc ggcatcactt ttatgagtcg agaacggtta ttgcgcgctg tcgcgtattt
    13381 tttcacaatt cccgggcaat tttcgtctga atgctgggca aaaagcgcaa aatccagaca
    13441 aaacaaaacg gcccgcaggc cgtttttcat aaaatcttta gcaacatact ggaaaagcgc
    13501 cgacagttgc aaatgcgttt ttacgcgatg cttcctgctc cacgacaggg aggagaggca
    13561 aagacggtgt cctgcaaccc ttcccactcc ttaatagtgt aagtatgcag agccagcgca
    13621 tgaacggtag tagagagttc ctccgctaaa gtactgtaaa tcattcgatg acgattcaga
    13681 aaacgttcac ccgtaaaacg atcgctgacc agcacaactt taaaatggct ttcagagccg
    13741 gctgggacat tgtgacgata gctttcatcc actacttcga ggaatacggg ttggaacgcc
    13801 gcccttaatt tttcttctat ccgctcacgt atcatcatga aatatctcct ccgacaacgc
    13861 tgagatgtca tccatccctt taaatactag ccgcttttac cgtttccatt gcagcttaac
    13921 aacaaatatt taggtttcgt cctggttttt ccgtcaagcg tatgaagttt actgatttaa
    13981 agattgaaac acaggcctcg catcagcgtt tcttttacca ggggcgatta tttcgtcaac
    14041 atgatgaatt tacatgcctt acctacttcc cctcgccgtt ggcaatgtta tgatggcggg
    14101 aatttttacc aaacacgctt aagagtctct gagagcccga acatgttcaa aaaaatcctc
    14161 ttcccgttag ttgctctgtt tatgcttgca ggatgcgcaa gaccgccaac aactattgaa
    14221 gtttccccga cgattacact gccacagcag gatccaagcc tgatgggcgt caccgtaagc
    14281 attaatggtg ccgatcagcg taccgatcag gcgctggcaa aagtcacccg cgataatcaa
    14341 atcgttaccc tgaccgcctc ccgcgatctg cgtttcctgc tgcaagaagt gctggaaaaa
    14401 cagatgaccg cgcgtggtta catggttggt ccgaatggcc cggttaatct gcaaatcatc
    14461 gttagccagc tgtatgctga cgtgtcccag ggcaacgtgc gctacaacat cgctaccaaa
    14521 gcggatatcg ccatcatcgc taccgcgcag aatggcaaca aaatgaccaa aaactatcgt
    14581 gccagctaca acgttgaagg tgcgttccag gcctccaaca aaaatatcgc cgatgcggtt
    14641 aacagtgtgc tgaccgatac catcgctgat atgtctcagg acaccagcat tcacgaattc
    14701 atcaagcaga acgcgcgtta atttctgccc tctggcccgg tgcaagccgg gcctgtagac
    14761 gcccatgtcc agtcaatatt tacgtatttt tcaacagccg cgttcagcca tattgctgat
    14821 cctgggtttt gcttccgggc taccgctcgc cctgacatcc ggcaccttac aggcctggat
    14881 gacggtcgag aatatcgatc tcaaaaccat tggtttcttc tctctggtag gccaggctta
    14941 cgtttttaaa ttcctctggt caccgctgat ggaccgctac acgcccccat tttttgggcg
    15001 gcggcgcggt tggctgctcg ccacgcaaat cctgttatta gtcgccattg cggcgatggg
    15061 ttttctcgaa ccaggcaccc aactccgctg gatggcggcg ctggcggtgg tgatcgcttt
    15121 ttgctctgcc tcccaggata ttgtcttcga tgcgtggaaa accgatgtgc tcccggcaga
    15181 agaacgtggt gcgggcgcgg caatcagcgt tctgggttac cgtttaggga tgctggtttc
    15241 cggcggccta gccctgtggc tggcagataa atggctgggc tggcagggca tgtactggtt
    15301 gatggcggcg ctgttgatcc cctgtattat cgcgacgttg cttgcaccag aaccaaccga
    15361 caccattcct gtgcccaaaa cgctggaaca agcggttgtt gcacctctgc gagatttctt
    15421 tggtcgcaat aatgcctggc ttattttgct tcttatcgtg ctgtataagc tgggcgacgc
    15481 attcgccatg agcctgacaa ccacgttttt gattcgcggc gtcgggtttg atgcgggtga
    15541 agtaggcgtg gtaaacaaaa cgcttggctt actagcgacc attgttggcg cattgtacgg
    15601 tgggattttg atgcagcgcc tgtcactgtt ccgggcactg ctgattttcg gcattttaca
    15661 aggtgcgtct aacgctggtt actggctgct gtcgattact gataagcatc tctacagcat
    15721 gggcgcagcc gtctttttcg aaaacctctg tggcgggatg ggcacatcag cctttgtcgc
    15781 gctgttaatg acgctatgta ataagtcatt ttccgctact caatttgccc tgctctcagc
    15841 gctttctgct gtagggcgag tttatgtcgg ccccgtggcg ggttggtttg ttgaagcaca
    15901 cggctggtcg acattctatc tattctccgt cgccgctgcc gtaccagggc ttattttgct
    15961 gctggtttgc cgccagacgc ttgaatatac acgagtaaat gacaacttta tctcccgtac
    16021 cgcatatccg gcaggttatg cctttgccat gtggacactg gcggcgggcg tcagcctgtt
    16081 ggccgtgtgg ttactgctat tgacgatgga cgcgctggat ttgacgcact tctctttcct
    16141 gcctgctctg ctggaagtcg gggttttagt cgccctttct ggcgtcgtgc ttggtggttt
    16201 gctggattat ctggcgctac gaaaaacgca tctgacgtaa tctgtaaata ttatttagct
    16261 gtagtaatca ctcgccgtaa ttattacggc taaataaaca tcagcatcgc ttattaatat
    16321 ttgttctttt gtgcggctta gcgtttggaa attattggcg ccatttataa attctatttt
    16381 tcttacacga ttcagctaat gagtctttat tttctcacca ccaagttgtc actccaatga
    16441 taattatttg ttaaataatt gttttatttc gcattgatta taccaattgc ccgtccagac
    16501 ttcttctgca ctcccctttt gttataacgc ccttttgcaa cagcttctta aaatcaacct
    16561 gatatgtttt gcaacatatg tgacctgaca gccaaatcca agtaacagga atttaatcat
    16621 gtttacagta atgtaacctt cccgtaaaat gcccacacat tttaaacgcc accagatccc
    16681 gtggaattga ggtcgttaaa tgagactcag gaaatacaat aaaagtttgg gatggttgtc
    16741 attatttgca ggcactgtat tgctcagtgg ctgtaattct gcgctgttag atcccaaagg
    16801 acagattggt ctggagcaac gttcactgat actgacggca tttggcctga tgttgattgt
    16861 cgttattccc gcaatcttga tggctgttgg tttcgcctgg aagtaccgtg cgagcaataa
    16921 agatgctaag tacagcccga actggtcaca ctccaataaa gtggaagctg tggtctggac
    16981 ggtacctatc ttaatcatca tcttccttgc ggtactgacc tggaaaacca ctcacgctct
    17041 tgagcctagc aagccgctgg cacacgacga gaagcccatt accatcgaag tggtttccat
    17101 ggactggaaa tggttcttca tctacccgga acagggcttt gctaccgtga atgaaatcgc
    17161 tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga tgaactcctt
    17221 cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga ctcgcctgca
    17281 tctgatcgcc aacgaacccg gcacttatga cggtatctcc gccagctaca gcggcccggg
    17341 cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgcat tcgaccagtg
    17401 ggtcgcaaaa gcgaaacagt cgccgaactc catgtctgac atggcagcgt tcgaaaaact
    17461 ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac caaacttgtt
    17521 tgccgatgta attaacaagt ttatggctca cggtaagagc atggacatga cccagccaga
    17581 aggtgagcac agcgcacacg aaggtatgga aggcatggac atgagccacg cggaatccgc
    17641 ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg atgcagtccc
    17701 gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag gtctggcgct
    17761 cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt ggctgacctc
    17821 cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga tgttgctgcg
    17881 tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg cgggcgaagc
    17941 gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg tgattatgat
    18001 cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ttggtggttc cgctgcagat
    18061 cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt ttaccgttgt
    18121 tggtgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga ccggctggct
    18181 ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgtcg attactggat
    18241 atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact tcttcgttac
    18301 cattctgaag atgcgcgcac cgggcatgac catgttcaag atgccagtat ttacctgggc
    18361 atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg ttaccgtcgc
    18421 gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata tgggtggcaa
    18481 catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt acatcctgat
    18541 cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta aacgtctgtt
    18601 tggttatacc tcgctggtat gggcaaccgt ctgtatcacc gtgctgtcgt tcatcgtttg
    18661 gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct ttggtatcac
    18721 cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt tcaccatgta
    18781 tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta tcgtcacctt
    18841 ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact tcgttctaca
    18901 taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg tggtcttcgg
    18961 ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac tgaacgaaac
    19021 ctggggtaaa cgcgcgtttt ggttctggat catcggcttc ttcgttgcct ttatgccact
    19081 gtacgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg acccgcagtt
    19141 ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctag gtattctctg
    19201 cctcgttatt cagatgtacg tttctattcg tgaccgcgac cagaaccgtg acctgactgg
    19261 cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc cgttctataa
    19321 ctttgccgtt gtgccgcacg ttcacgagcg tgatgcattc tgggaaatga aagagaaagg
    19381 cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa acagcggtgc
    19441 aggtatcgtc attgcggctt tctccaccat cttcggtttc gccatgatct ggcatatctg
    19501 gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga aaagcttcga
    19561 cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa accagcattt
    19621 cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg cacgcgactg
    19681 tccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc ggattttgga
    19741 tctacctgat gagcgactgc attctgttct ctatcttgtt tgctacctat gccgttctgg
    19801 tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg ttcgttctgg
    19861 ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct atcgccatgt
    19921 acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg tttggcgccg
    19981 gatttatcgg gatggaaatc tacgaattcc atcacctgat tgttaacggc atgggtccgg
    20041 atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt ctgcacgtca
    20101 cttccggtct tatctggatg gcggtgctga tggtacaaat cgctcgtcgc ggcctgacca
    20161 gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg gatgtggttt
    20221 ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc attctaccga
    20281 tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta tcctgtcgat
    20341 cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc cggccgtaat
    20401 tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg tgtgcttcct
    20461 gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct tcaccgtgct
    20521 aatcatcgct atcctagttg taggctccat ctggattatg tggaacctca actacaacat
    20581 gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt aacgaaacca
    20641 ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc ctcaaagggc
    20701 agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt tgtggcgtcg
    20761 ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga aaggacgaag
    20821 aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta cgccaccttg
    20881 ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc ctgctggctg
    20941 ggggtgatgg gctttgtggt ttatgtcggc gtttatagcc tgtacatgaa acgccactcc
    21001 gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgccggtgat tggctactgt
    21061 gcagtaaccg gcgagttcga tagcggcgca gcgatcctgc tggctatctt cagcctgtgg
    21121 cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca ggcggcaaac
    21181 attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat cacgctgtat
    21241 atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc tggatataaa
    21301 tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct gcgcggttat
    21361 aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat catcgccatc
    21421 actgccctct cggtgatgat gtccgttgat tttatggtgc cggactcgca tacgctgctg
    21481 gctgctgtgt ggtaacacaa cctctctatt aaaaaggtgc tacggcacct tttttcttag
    21541 cattagaaac atatccctct cgaaatattt actaaaaaat ccgcatgttt accccattcg
    21601 tttgccgctt tacactagtc gcgaatttaa aacagaggtg gtaatgaacg attataaaat
    21661 gacgccaggt gagcggcgcg cgacctgggg tttagggacc gtattctcgt tgcgcatgct
    21721 gggcatgttc atggttctgc cggttctgac cacgtacggc atggctctgc aaggtgccag
    21781 cgaagcatta atcggtattg ccattggtat ttatggtctg actcaggccg tttttcagat
    21841 tccgtttggc ctgctttccg accgcattgg tcgcaaacca ttaattgtcg gtgggctggc
    21901 agtgtttgcc gccggtagcg ttatcgctgc gctctctgac tccatctggg gaattattct
    21961 gggtcgggcg ctacaaggct ccggtgcgat tgccgctgcc gttatggcgc tgctttccga
    22021 tctcacgcgc gaacaaaacc gcaccaaagc aatggcgttt atcggcgtga gctttggcat
    22081 tacctttgcc attgcgatgg tgcttggccc gatcatcact cacaaacttg ggctgcacgc
    22141 gctgttctgg atgatcgcta ttctggcaac gaccggcatt gcgttgacca tttgggttgt
    22201 gcccaacagt agcactcacg tacttaatcg tgagtccgga atggtgaaag gcagtttcag
    22261 taaggtgctg gcggaaccgc ggctgctgaa actcaacttt ggcattatgt gtctgcatat
    22321 tttgctgatg tcgacgtttg ttgccctgcc cggacaactg gctgatgcgg ggttcccggc
    22381 ggctgaacac tggaaggtct atctggcgac aatgctaatc gcctttggct cggtcgtgcc
    22441 tttcattatc tacgctgaag ttaagcgcaa aatgaagcaa gtctttgtct tctgcgtcgg
    22501 gttgatcgtg gttgcggaaa ttgtgttgag gaacgcgcaa acgcagttct ggcaactggt
    22561 ggtcggcgtg cagcttttct ttgtggcgtt taatttgatg gaagccctcc tgccctcact
    22621 tatcagtaaa gagtcgccag caggttacaa aggtacggcg atgggtgttt actccaccag
    22681 ccagtttctt ggcgtggcga ttggcggttc gctgggcggc tggattgacg gcatgtttga
    22741 cggtcagggg gtatttctcg ctggcgcaat gctggccgca gtgtggctgg cagtcgccag
    22801 taccatgaaa gaaccgccgt atgtcagcag tttgcgcatt gaaatcccgg cgaacattgc
    22861 cgcaaacgag gcgttaaaag tgcgtttgct ggaaactgaa ggcatcaaag aagtgttgat
    22921 tgcagaagaa gaacattcag cttatgtgaa aatcgacagc aaagtgacga atcgctttga
    22981 tgttgaacag gcaattcgcc aggcataaaa gcgaggcgaa attgcctgct gcgctacgct
    23041 tatcaggcct actaagttcc tgatttacaa aactttgtag gccgaaccag gcgttcatgc
    23101 cgcatctgac atgaacaaag cgcaggttgt cgcgattaat cgcggaagtt tttgaactgg
    23161 aacggctgac cgagatcgcc accgcgcacc atcgccatga cagcctgtaa atcatcacga
    23221 gatttgcccg tcacacggat ctcatcgccc tgaatttgcg cctgcacttt cagtttgctg
    23281 tctttgatca tcttgacgat ttttttctga gtggcgctct caatgccctg tttcagtttc
    23341 gcttccacaa accaggtttt accgctatga acgatatttt ccggtacatc cagtgaactg
    23401 ccttcaatgc cgcgcttcag cagcttggca cgcagaatat ccagcaactg attgacctgg
    23461 aagtcggact cgctcaacac tttgatggtt ttgctggcgt cgttcagctc aaatgaggct
    23521 tcaacgttac ggaagtcaaa acgggactcc acttcgcggc tcgcgttatc gaccgcgtta
    23581 cgtgcttcct gaagatcaac ttcagagaca atatcgaaag atggcatctt ttcctctccc
    23641 ttcatttttg atgcgaagca taatacctgc aaagttaaat aaccaacagt cctgcaccag
    23701 gacgtatccc ttaattgtgc atagtcgcaa cagcatgcac aattaaggga tagccttatt
    23761 gaacacccgg agtggttgcg ggtgaggagg aacaatgaaa attaccgtat tgggatgcgg
    23821 tgccttaggg caattatggc ttacagcact ttgtaaacag ggtcacgaag ttcagggctg
    23881 gctgcgcgta ccgcaacctt attgtagtgt gaatctggtt gagactgatg gttcgatatt
    23941 taacgaatcg ctgaccgcca acgatcccga ttttcttgcc accagcgatc tgctcctggt
    24001 gacgctgaaa gcatggcagg tttccgatgc cgtcaaaagc ctcgcgtcca cactgcctgt
    24061 aactacgcca atactgttaa ttcacaacgg catgggcacc atcgaagaat tgcaaaacat
    24121 tcagcagcca ttactgatgg gcaccaccac ccatgccgcc tgccgcgacg gcaatgtcat
    24181 tattcatgtg gcaaacggta tcacgcatat tggtccagca cggcaacagg acggcgatta
    24241 cagttatctg gcggatatat tgcaaaccgt gttgcctgac gttgcctggc ataacaatat
    24301 tcgcgccgag ctgtggcgca agctggcagt caactgtgtg attaatccac tgactgccat
    24361 ctggaattgc ccgaacggtg aattacgtca tcatccgcaa gaaattatgc aaatatgcga
    24421 agaagtcgcg gcagtgatcg aacgcgaagg gcatcatact tcagcggaag atttacgtga
    24481 ttacgtgatg caggtgattg atgccacagc ggaaaatatc tcgtcgatgt tgcaggatat
    24541 ccgcacgctg cgccacaccg aaatcgacta tatcaatggt tttctcttac gccgcgcccg
    24601 cgcgcatggg attgccgtac cggaaaacac ccgcctgttt gaaatggtaa aaagaaagga
    24661 gagtgaatat gagcgcatcg gcactggttt gcctcgcccc tggtagtgaa gagactgaag
    24721 ccgtcaccac gatcgacctc ttgattcgcg gcggtatcaa agtcaccact gccagcgtag
    24781 ccagcgatgg caacctggcg attacctgct cgcgcggcgt gaagctgctg gcggatgcgc
    24841 cgctggtcga agtggctgat ggcgaatatg acgtgatcgt gctgcctggt ggcattaaag
    24901 gcgcggagtg ttttcgcgat agtaccctgc tggttgaaac cgttaaacag ttccaccgtt
    24961 ccgggcgtat cgtcgcggct atttgcgccg cgccagccac cgtgctggtg ccgcacgata
    25021 tctttccgat tggcaatatg accggcttcc cgacgctgaa agacaaaatt cccgctgaac
    25081 aatggcagga caagcgcgtc gtctgggatg cacgggtaaa attgctgacc agccaggggc
    25141 cgggtacagc tatcgacttt ggtctgaaaa ttatcgacct gctggttggg cgcgagaaag
    25201 cccatgaagt ggcatcacaa ctggtgatgg cggcagggat ttataattat tacgagtagt
    25261 gtcggatgcg gcaaacgtcg catctgacca gatgcgacgt aaaaaccaca aattacggac
    25321 gatacacctt cacattctta aagccctgtt cgcgtagata gagcgcctgc aggcggctca
    25381 ttaccccgcg ctcacaccac agtagccagg ttctgttctg gtcgagatcg ccaaatttgg
    25441 tgctcaactt gtagaacggc agtgaaacca catcaatccc ttcgactttc agtggcttat
    25501 cttcctgttc atcgacagaa cggatatcga gaatcacgtc gttcgggccg aagtcattga
    25561 cggtttccac ttccaccact tcctgcccgg tctgctgggc gatttcgcgg atatcaacgt
    25621 tattcgcttc ttcaaccact ttatcgagaa tgctgaagtc gaatttctct tcttccgctt
    25681 caatcttcga cttaaccgct ttcaccgtcg ggcttttgga gatcacaccg cagtattccg
    25741 gcatagtacg ggcaaagtct tcggtgccaa tctggcgagc caggttgatg atgtgctctt
    25801 tgtcgtaaga gatcagcgga cgcaggatca gcgtatcaga gacgttatca atcaggcgca
    25861 ggttggtcag cgtctgactg gacacctggc cgagcgcttc gccggtgacc agcgcctgta
    25921 cgccgtaacg ttcagccact ttagacgcgg cacgcaccat catacgtttg aggataacgc
    25981 ccatctgacc gtcgtcgatt ttctcgagaa tttccccgac taccggctcg aaattaatag
    26041 cgacaaaacg cacgcggtgg gagctgccaa agcggttcca cagataatgt gccacctgac
    26101 gcacgccaat ttcatgcgct gcgccgccga ggttaaagaa gcagtaatgc acgcggcagc
    26161 cgcgacgcat caacatataa ctggaaacac cggagtcgaa accaccggaa atgagcgaca
    26221 gcacatcttc ctgggtgccg atcgggaaac cgccaatacc ttcgtagcgg cctttaatca
    26281 gcaggagacg atcgtcttcc acttccagat ggacagtcac atccggattg gtcagcttca
    26341 cgcgcgcgga ttcaatatgc tgatttaaac cgccgccgac gtaacgttcc acatcaatcg
    26401 agctaaaatc atgtttgcca cggcgcttca cgcgtacgca gaaggttttg ccatccagct
    26461 gatcgcgata ctgaaccaac gctttctcga aaatatcgtg catgtcggta aacggcacgt
    26521 cttcgacttc gagaatatgg tggataccag gaatacgggt cagggcgtcg cgaatagtca
    26581 gacgctggtt ttcatctttc gcgcgaactt cgatgttatc ccagtggcgg acgacagcga
    26641 gcgtctcatc atagtgcttt aaaacgttac gaatgttccc ggtaaggatt tttataaagc
    26701 gcaagcgcac agattggctt ttgatggtga tttccgggaa caatttaatg ataaacttca
    26761 tggcggcaat ggttcgttgg caagccttaa gcgacttgta tagggaaaaa tacagcagcc
    26821 cacacctgcg gctgcatcca ggcgcggaag tataccacta acatcgcttt gctgcgcaca
    26881 tcaccttacc attgcgcgtt atttgctatt tgccctgagt ccgttaccat gacggggcga
    26941 aaaatattga gagtcagaaa ttcattatgc cgaagaaaaa tgaggcgccc gccagctttg
    27001 aaaaggcgct gagcgagctg gaacagattg taacccgtct ggaaagtggc gacctgccgc
    27061 tggaagaggc gctgaacgag ttcgaacgcg gcgtgcagct ggcacgtcag gggcaggcca
    27121 aattacaaca agccgaacag cgcgtacaaa ttctgctgtc tgacaacgaa gacgcctctc
    27181 taaccccttt tacaccggac aatgagtaat ggactttccg cagcaactcg aagcctgcgt
    27241 taagcaggcc aaccaggcgc tgagccgttt tatcgcccca ctgccctttc agaacactcc
    27301 cgtggtcgaa accatgcagt atggcgcatt attaggtggt aagcgcctgc gacctttcct
    27361 ggtttatgcc accggtcata tgttcggcgt tagcacaaac acgctggacg cacccgctgc
    27421 cgccgtagag tgtatccacg cttactcatt aattcatgat gatttaccgg cgatggatga
    27481 tgacgatctg cgtcgcggct tgccgacctg ccatgtgaag tttggcgaag cgaacgcgat
    27541 tctcgctggc gacgctttac aaacgctggc gttctcgatt ttaagcgatg ctgatatgcc
    27601 agaagtgtcg gatcgcgaca gaatttcgat gatttctgaa ctggcgagcg ccagcggtat
    27661 tgccggaatg tgtggtggtc aggcgttgga tttagacgcg gaaggcaaac acgtacctct
    27721 ggacgcgctt gagcgtattc atcgtcataa aacaggcgca ttaattcgcg ccgccgttcg
    27781 ccttggtgca ttaagcgccg gagataaagg acgtcgtgct ctaccagtac tcgacaagta
    27841 cgcagagagc atcggccttg ccttccaggt tcaggatgac atcctggatg tggtgggaga
    27901 tactgcaacg ttgggaaaac gtcagggtgc cgaccagcag cttggcaaaa gtacctaccc
    27961 tgcactcctg ggtcttgagc aagcccggaa gaaagcccag gatctgatcg acgatgcccg
    28021 ccagtcgctg aaacaactgg ctgaacagtc gatcgatact tcggcactgg aagcgctagc
    28081 ggactacatc atccagcgta ataaataaac aataagtgtt aataggcccc tgatgagttt
    28141 tgatattgcc aaatacccga ccctggcact ggtcgactcc acccaggagc tacgactgtt
    28201 gccaaaagag agtttaccga aactctgcga cgaactgcgc cgctatttac tcgacagcgt
    28261 gagccgttcc agcgggcact tcgcctccgg gctgggcacg gtcgaactga ccgtggcgct
    28321 gcactatgtc tataacaccc cgtttgacca attgatttgg gatgtggggc atcaggctta
    28381 tccgcataaa attttgaccg gacgccgcga caaaatcggc accatccgtc agaaaggcgg
    28441 tctacacccg ttcccgtggc gcggcgaaag cgaatatgac gtattaagcg tcgggcattc
    28501 atcaacctcc atcagtgccg gaattggtat tgcggttgct gccgagaaag aaggcaaaaa
    28561 tcgccgcacc gtctgtgtga ttggcgacgg cgcgattacc gcaggcatgg cgtttgaagc
    28621 gatgaatcac gcgggcgata ttcgtcctga tatgctggtg gtcctcaacg acaatgaaat
    28681 gtcgatttcc gaaaatgttg gcgcgcttaa caaccatctg gcgcagctgc tttccggtaa
    28741 gctttactct tcactgcgcg aaggcgggaa aaaagttttc tctggcgttc cgccaattaa
    28801 agagctgctc aaacgtaccg aagaacatat taaaggcatg gtggtgcctg gcacgttgtt
    28861 tgaagagctg ggctttaact acatcggccc ggttgacggt cacgatgtgc tggggcttat
    28921 caccacgctg aagaacatgc gcgacctgaa aggcccgcag ttcctgcata tcatgaccaa
    28981 aaaaggtcgt ggttatgaac cggcagaaaa agacccaatc accttccacg ccgtgcctaa
    29041 atttgatccc tccagcggtt gtttgccgaa aagtagcggt ggtttaccaa gctattcaaa
    29101 aatctttggc gactggttgt gcgaaacggc agcgaaagat aacaaactga tggcgattac
    29161 tccggcgatg cgtgaaggtt ccggcatggt cgagttttca cgtaaattcc cggatcgcta
    29221 ttttgatgta gcgattgccg agcaacacgc ggtgaccttt gccgccggtc tggcgattgg
    29281 tggctacaaa ccgatcgtgg caatttactc caccttcctg caacgcgcct atgatcaggt
    29341 gctgcatgac gtggcaattc aaaaactccc ggtcctgttc gccatcgacc gcgcgggcat
    29401 tgttggtgct gacggtcaaa cccaccaggg cgcttttgat ctctcttacc tgcgctgcat
    29461 accggaaatg gtcattatga ccccgagcga tgaaaacgaa tgtcgccaga tgctctatac
    29521 cggctatcac tataacgatg gcccgtcagc ggtgcgctac ccgcgtggca acgcggtcgg
    29581 cgtggaactg acgccgctgg aaaaactacc aattggcaaa ggcattgtga agcgtcgtgg
    29641 cgagaaactg gcgatcctta actttggtac gctgatgcca gaagcggcga aagtcgccga
    29701 atcgctgaac gccacgctgg tcgatatgcg ttttgtgaaa ccgcttgatg aaacgttaat
    29761 tctggaaatg gccgccagtc atgaagcact ggttaccgta gaagaaaacg ccattatggg
    29821 cggtgcaggc agcggagtga acgaagtgct gatggcccat cgtaaaccag tacccgtgct
    29881 gaacattggc ctgccggact tctttattcc gcaaggaact caggaagaaa tgcgcgccga
    29941 actcggcctc gatgccgccg gtatggaagc caaaatcaag gcctggctgg cataatccct
    30001 actccactcc tgctatgctt aagaaattat tcatggcagg agtggcagca tgcaatacaa
    30061 ccccttagga aaaaccgacc ttcgcgtttc ccgactttgc ctcggctgta tgacctttgg
    30121 cgagccagat cgtggtaatc acgcatggac actgccggaa gagagcagcc gtcccatcat
    30181 taaacgcgca ctggaaggcg gcataaattt ctttgatacc gccaatagct attccgatgg
    30241 cagcagcgaa gagatcgtcg gtcgcgcact gcgggatttc tcccgtcgtg aagacgtggt
    30301 cgttgccacc aaagtgttcc atcgcgttgg tgatttaccg gaaggattat cccgtgcaca
    30361 aattttgcgc tctatcgacg acagcctgcg acgtctcggc atggattatg tcgatatcct
    30421 gcaaattcat cgctgggatt acaacacgcc gatcgaagag acgctggaag ccctcaacga
    30481 cgtggtaaaa gccgggaaag cgcgttatat cggcgcgtca tccatgcacg cttcgcagtt
    30541 tgctcaggca ctggaactac aaaaacagca cggctgggcg cagtttgtca gtatgcagga
    30601 tcactacaat ctgatttatc gcgaagaaga gcgcgagatg ctgccgctgt gttatcagga
    30661 aggcgtggcg gtcattccgt ggagtccgct ggcgcggggg cgactgacgc gtccgtgggg
    30721 agaaactacc gcacgactgg tgtctgatga ggtggggaaa aatctctata aagaaagcga
    30781 tgaaaatgac gcgcagatcg cagagcggtt aacgggcgtc agtgaagaac tcggtgcaac
    30841 acgagcacaa gttgcgctgg cctggttgtt gagtaaaccg ggcattgccg caccgattat
    30901 cggaacttcg cgggaagaac agcttgatga gctgctgaac gcggtggata tcactttgaa
    30961 gccggaacag attgctgaac tggaaacacc gtataaaccg catccggtcg taggatttaa
    31021 gtaaatttcg cttttcaccc attttatctg ctgaattccc catcgtctgc gatgatgggg
    31081 acaacgtacc tcccatcacc ttctgtaacc ataatccacc tctgcaagag aaattcttcg
    31141 tgaatgagtt tgcagctatt catcgtcttc atccctttga gcgatttttg atagtttctc
    31201 caggtaatat cccctaaaac tgtcttattt agtcaacgta agaggattac aatcatgatg
    31261 aaaaccatca ccaaacaacc gatcctgttt actgatgtac cggttgcaga tttaagaaac
    31321 tcaatgaagc aagatttaaa tcaaaatctt attgaaaggc tgtggaacaa aattcgtgat
    31381 ttttttctgg acagtgataa acaaaaggca tttaaatcga ttcataaata catcaataca
    31441 ttgtcagttc taaattataa ttcagcgtta actccagatc ccaactttaa tatagatgca
    31501 acgtcagatt tagactcata tttaaagctg gattttgatc gcctctcgcc caaacaaaaa
    31561 cagaccacgc tttgttgttt ctggaataaa attgcatcct cattgccaga gccatacaac
    31621 agtaccatca aacataacat tattttttat aaagacggag aaaatctgat gatacgtgga
    31681 actatctcta ttgttaacga ggtagtaaag acttattcac tcccaataga aaaggatgac
    31741 aatggatatt acgatttcag cggtctatat ctagcacaca gcaatatttc cggtaaagat
    31801 cccaataaag atcccgatat agatttcggt atagatatgg gaaactgtaa ctgctcaaat
    31861 gtcaacttcg aacatactta tttttatggc gtcaagttta caaatgcgaa ttgtacaaat
    31921 gcgaatttta acaattgtag atttaagaaa tgcgacctga caaatatgaa ttgcactggc
    31981 gcaattcttg ataatgcgat gatatatggt aaagaaaaag aacctgagat gcaatatcca
    32041 gaagcagatc aaataataca aaggataact tatcagaaat cagatgggaa tgaaacgaaa
    32101 gggatgattc taactaactg ctcc
//
LOCUS       DBFIFM010000034        32084 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.034, whole genome shotgun sequence.
ACCESSION   DBFIFM010000034 DBFIFM010000000
VERSION     DBFIFM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.034"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..230
                     /locus_tag="N7C74_001382"
     CDS             <1..230
                     /locus_tag="N7C74_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2431280.1"
                     /translation="AWLLWWAITILVLFILSLTTTLNLRSIGIGLSFGFCQILGRYTM
                     SLGWSKYWGVLGGFPVTNIVLFLILIFFKKR"
     gene            complement(277..1164)
                     /gene="rarD"
                     /locus_tag="N7C74_001383"
     CDS             complement(277..1164)
                     /gene="rarD"
                     /locus_tag="N7C74_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HGU2431281.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPTDEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1216..1683)
                     /gene="yigI"
                     /locus_tag="N7C74_001384"
     CDS             complement(1216..1683)
                     /gene="yigI"
                     /locus_tag="N7C74_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HGU2431282.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1848..2717
                     /gene="pldA"
                     /locus_tag="N7C74_001385"
     CDS             1848..2717
                     /gene="pldA"
                     /locus_tag="N7C74_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HGU2431283.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2850..4679
                     /gene="recQ"
                     /locus_tag="N7C74_001386"
     CDS             2850..4679
                     /gene="recQ"
                     /locus_tag="N7C74_001386"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HGU2431284.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4743..5363
                     /gene="rhtC"
                     /locus_tag="N7C74_001387"
     CDS             4743..5363
                     /gene="rhtC"
                     /locus_tag="N7C74_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HGU2431285.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5425..6045)
                     /gene="rhtB"
                     /locus_tag="N7C74_001388"
     CDS             complement(5425..6045)
                     /gene="rhtB"
                     /locus_tag="N7C74_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HGU2431286.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6156..7178
                     /gene="pldB"
                     /locus_tag="N7C74_001389"
     CDS             6156..7178
                     /gene="pldB"
                     /locus_tag="N7C74_001389"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HGU2431287.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     IHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7186..7986
                     /gene="yigL"
                     /locus_tag="N7C74_001390"
     CDS             7186..7986
                     /gene="yigL"
                     /locus_tag="N7C74_001390"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HGU2431288.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8062..8961
                     /gene="bioP"
                     /locus_tag="N7C74_001391"
     CDS             8062..8961
                     /gene="bioP"
                     /locus_tag="N7C74_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HGU2431289.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8849..9802)
                     /gene="metR"
                     /locus_tag="N7C74_001392"
     CDS             complement(8849..9802)
                     /gene="metR"
                     /locus_tag="N7C74_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HGU2431290.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10038..12299
                     /gene="metE"
                     /locus_tag="N7C74_001393"
     CDS             10038..12299
                     /gene="metE"
                     /locus_tag="N7C74_001393"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HGU2431291.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPV
                     LLGPITWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYVQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSMTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12339..13154)
                     /gene="ysgA"
                     /locus_tag="N7C74_001394"
     CDS             complement(12339..13154)
                     /gene="ysgA"
                     /locus_tag="N7C74_001394"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HGU2431292.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGLDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAASAEDGWQRMLEWFKQYGGKKSL"
     gene            13416..14177
                     /gene="udp"
                     /locus_tag="N7C74_001395"
     CDS             13416..14177
                     /gene="udp"
                     /locus_tag="N7C74_001395"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HGU2431293.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            14318..15745
                     /gene="rmuC"
                     /locus_tag="N7C74_001396"
     CDS             14318..15745
                     /gene="rmuC"
                     /locus_tag="N7C74_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HGU2431294.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15840..16595
                     /gene="ubiE"
                     /locus_tag="N7C74_001397"
     CDS             15840..16595
                     /gene="ubiE"
                     /locus_tag="N7C74_001397"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HGU2431295.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16609..17214
                     /gene="ubiJ"
                     /locus_tag="N7C74_001398"
     CDS             16609..17214
                     /gene="ubiJ"
                     /locus_tag="N7C74_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HGU2431296.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALAGLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALIKRLEKLEAK"
     gene            17211..18851
                     /gene="ubiB"
                     /locus_tag="N7C74_001399"
     CDS             17211..18851
                     /gene="ubiB"
                     /locus_tag="N7C74_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HGU2431297.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18930..19199
                     /gene="tatA"
                     /locus_tag="N7C74_001400"
     CDS             18930..19199
                     /gene="tatA"
                     /locus_tag="N7C74_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HGU2431298.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19203..19718
                     /gene="tatB"
                     /locus_tag="N7C74_001401"
     CDS             19203..19718
                     /gene="tatB"
                     /locus_tag="N7C74_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HGU2431299.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19721..20497
                     /gene="tatC"
                     /locus_tag="N7C74_001402"
     CDS             19721..20497
                     /gene="tatC"
                     /locus_tag="N7C74_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HGU2431300.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20539..21321
                     /gene="tatD"
                     /locus_tag="N7C74_001403"
     CDS             20539..21321
                     /gene="tatD"
                     /locus_tag="N7C74_001403"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HGU2431301.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21318..21806)
                     /gene="rfaH"
                     /locus_tag="N7C74_001404"
     CDS             complement(21318..21806)
                     /gene="rfaH"
                     /locus_tag="N7C74_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HGU2431302.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21973..23466
                     /gene="ubiD"
                     /locus_tag="N7C74_001405"
     CDS             21973..23466
                     /gene="ubiD"
                     /locus_tag="N7C74_001405"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HGU2431303.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPIS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23512..24213
                     /gene="fre"
                     /locus_tag="N7C74_001406"
     CDS             23512..24213
                     /gene="fre"
                     /locus_tag="N7C74_001406"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HGU2431304.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24405..25568)
                     /gene="fadA"
                     /locus_tag="N7C74_001407"
     CDS             complement(24405..25568)
                     /gene="fadA"
                     /locus_tag="N7C74_001407"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HGU2431305.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25578..27767)
                     /gene="fadB"
                     /locus_tag="N7C74_001408"
     CDS             complement(25578..27767)
                     /gene="fadB"
                     /locus_tag="N7C74_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HGU2431306.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVIMKDINDKSLALGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFER
                     VDVVVEAIVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWHYKEDSKGKPKKEEDAAVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27957..29288
                     /gene="pepQ"
                     /locus_tag="N7C74_001409"
     CDS             27957..29288
                     /gene="pepQ"
                     /locus_tag="N7C74_001409"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HGU2431307.1"
                     /translation="MESLASLYKNHIATLQERTRDALTRFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQASEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29288..29902
                     /gene="yigZ"
                     /locus_tag="N7C74_001410"
     CDS             29288..29902
                     /gene="yigZ"
                     /locus_tag="N7C74_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HGU2431308.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29941..31392
                     /gene="trkH"
                     /locus_tag="N7C74_001411"
     CDS             29941..31392
                     /gene="trkH"
                     /locus_tag="N7C74_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HGU2431309.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31404..31949
                     /gene="hemG"
                     /locus_tag="N7C74_001412"
     CDS             31404..31949
                     /gene="hemG"
                     /locus_tag="N7C74_001412"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HGU2431310.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7453 a         8134 c         8767 g         7730 t
ORIGIN      
        1 aggcatggtt attgtggtgg gctattacca tccttgttct atttatttta agcctgacta
       61 cgacattaaa tttaagaagt ataggtatag ggttaagttt tgggttttgt caaatccttg
      121 gtagatatac gatgtctcta ggatggagta agtactgggg agttttgggg ggatttcctg
      181 taacaaatat tgttcttttt cttatcttaa tttttttcaa aaaaagataa ataagtcagt
      241 gggtaggctc tttaaacggc ctacccatgt taattcttaa ctttttctac gctgcgtata
      301 aatcgcatcc atcacaaaaa ttgccagcgc cacccaaata aaggtgaaag tcaccatctt
      361 atcggcaccc ggtttttcac cataaaacgt cacagccagc aggaacatca gcgtcgggcc
      421 aatgtactgg aaaaagccta acgttgagag acgaaggcgc gtggcagcgg cggtaaaaca
      481 caacagcggt acggtagtga caataccggc ggcgatcagc agtaaattca gcgacatcgg
      541 gttttgcccc atatggctgg ttgagctgtc ggcaatagca aacaggtaaa tcgccgccac
      601 gggcagcagc cacatggttt caattaacat gccggtttgt gcttctaccg caatcttctt
      661 gcgcaccagg ccgtagaagg caaaactaaa tgccagcccc agcgcgataa taggtagcga
      721 accaaaagtc cacagctgga ctaacacgcc acatatcgcc agaatcaccg ccagccattg
      781 catccggcgg aatcgctcgc cgaggaaaat catccccagc acaatgttca ccagcgggtt
      841 aataaagtaa ccaaggctcg cttccagcat atggtgattg ttcaccgccc agataaacag
      901 tagccagttg ccaccaatca gcacggcaga gactgccagc ataaaaattt tctgtggcgt
      961 ctgaatcagc gtttttaaat aggaccactg gcggcaaatg ctcatcagca ccaccataaa
     1021 gaaaaacgac cagatcacgc gatgcgtcag gatctcatcg gtgggcacgt agtaaatcaa
     1081 cttgaagtac gctggggcta taccccaaat aaaataagcg gcaagagcga gtaatacgcc
     1141 ctgccgcgtt tgttttgcat ccatcgggaa tactcatttt taattggtaa cagcagttta
     1201 cctgctttta tgtcttcaac ctaccatata ggtggcggtg gcactggcaa tataaagctg
     1261 ttcttcattg tgtaattcaa cgcgggcgac ggcgacttta ttgcctgcac gcaacaggct
     1321 actggtagca gtaaaacgct cgcctctgcc tgggcgcaga taatcgacgc gaagatcaat
     1381 ggtccccatc cgcgatagcc gctggcgtag ttcatcttca ctgatggttt cgtggcgggt
     1441 taaggtactt cctacgcaca ccagaccggc ggcgacatcc agcgccgacg caatgacccc
     1501 gccgtgcaaa atgctttgcg cccagttgcc caccatcatt ggctgatttt taaaggccag
     1561 ctgtgcgaac tctttttcgt aacgctccag ttccatcccc aatgcgcggt taaatggcat
     1621 gtgataaaca aacatctcac ccactaattt cagggcttgt tcagcggtca gtacggcaga
     1681 catatcatcc ttacacttca ttggttaatg aaatgttgat tttatgattc tttgttgttg
     1741 gtttctactt taggaaggga taactaacgg caatggagtt aagtatgtaa aataccccgc
     1801 agaaaaacat tcaccttatc aataattcgt tacggagaac acgaccgatg cggactctgc
     1861 agggctggtt gttgccggtg tttatgttgc ctatggcagt atatgcacaa gaggcaacgg
     1921 tgaaagaggt gcatgacgcg ccagcggtgc gtggcagtat tatcgccaat atgctgcagg
     1981 agcatgacaa tccgttcacg ctctatcctt atgacaccaa ctacctcatc tacacccaaa
     2041 ccagcgatct gaacaaagaa gcgattgcca gttacgactg ggcggaaaat gcgcgtaagg
     2101 atgaagtaaa gtttcagttg agcctggcat ttccgctgtg gcgtgggatt ttaggcccga
     2161 actcggtgtt gggtgcgtct tatacgcaaa aatcctggtg gcaactgtcc aatagcgaag
     2221 agtcttcacc gtttcgtgaa accaactacg aaccgcaatt gttcctcggt tttgccaccg
     2281 attaccgttt tgcaggttgg acgctgcgcg atgtggagat ggggtataac cacgactcta
     2341 acgggcgttc agacccgacc tcccgcagct ggaaccgcct ttatactcgc ctgatggcag
     2401 aaaacggtaa ctggctggta gaagtgaagc cgtggtatgt ggtgggtaat actgacgata
     2461 acccggatat caccaaatat atgggttact accagcttaa aatcggctat cacctcggtg
     2521 atgcggtgct cagtgcgaaa ggacagtaca actggaacac cggctacggc ggcgcggagt
     2581 taggcttaag ttacccgatc accaaacatg tgcgccttta tactcaggtt tacagcggct
     2641 atggcgaatc gctcatcgac tataacttca accagacccg tgtcggtgtg ggggttatgc
     2701 taaacgattt gttttgatga acggttgagt ggttggcaaa tctggaatcc agcatccagg
     2761 attaccctct cagagactaa aagcattgca gtttctcgcg caggcgctga aaatagcgcc
     2821 tgtttttatt tcaggcaatc ggggtgaatg tggcgcaggc ggaagtgttg aatctggagt
     2881 ccggagctaa acaggtttta caagaaacct ttggctacca acagtttcgc cccggccagg
     2941 aagaaattat cgacactgtg ctttccggtc gcgattgcct ggtcgtcatg cccaccggtg
     3001 gcggaaaatc cctttgctat caaattcctg ccttattgct aaacggcctt accgtggttg
     3061 tttcaccgct gatttcgttg atgaaagatc aggtggatca actgcaagcc aacggcgtgg
     3121 cggcggcgtg ccttaactcg acgcaaaccc gcgaacagca acttgaagtg atgacaggct
     3181 gccgcaccgg gcaaattcgt ctgctttata tcgccccgga acgcctgatg ctggataact
     3241 ttcttgagca tctggcgcac tggaatccgg tgttattagc cgttgatgaa gcgcactgta
     3301 tctcccaatg gggccacgat ttccgcccgg aatatgccgc gctcggtcag ttgcgccagc
     3361 ggttcccgac gctgccgttt atggcgctga ccgccacagc cgacgacacc acgcgccagg
     3421 atatcgtgcg cctgctgggg ctgaacgatc cgctgattca aatcagcagt tttgaccgtc
     3481 cgaatattcg ctacatgctg atggagaaat tcaaaccgct cgatcagttg atgcgctacg
     3541 tgcaggaaca gcgcggtaag tccggcatta tctactgcaa cagccgggcg aaagtagaag
     3601 acaccgctgc gcgcctgcaa agcaagggta ttagcgcggc ggcctatcat gccgggctgg
     3661 aaaataatgt ccgcgccgac gtgcaggaga aattccagcg cgatgacctg caaattgtgg
     3721 tggcgacggt ggcgttcggc atgggcatca ataaaccaaa cgttcgcttc gtggtccact
     3781 ttgatattcc gcgcaatatc gaatcctatt atcaggaaac cggtcgcgcc gggcgtgatg
     3841 gcctgcctgc ggaagcgatg ttgttttacg atccagctga tatggcgtgg ctgcgccgtt
     3901 gtctggaaga gaagccgcag gggcagttgc aggatatcga gcgccacaaa ctcaatgcga
     3961 tgggcgcgtt tgccgaagcg caaacttgcc gtcgtctggt attgctgaac tattttggcg
     4021 aagggcgtca ggagccgtgc gggaactgcg atatctgcct cgatccgccg aaacagtacg
     4081 acggttcaac cgatgctcag attgcccttt ccaccattgg tcgtgtgaat cagcggtttg
     4141 ggatgggtta tgtggtggaa gtgattcgtg gtgctaataa ccagcgtatc cgcgactatg
     4201 gtcatgacaa actgaaagtc tatggcatgg gccgtgataa aagccatgaa cattgggtga
     4261 gcgtgatccg ccagctgatt cacctcggcc tggtgacgca aaatattgcc cagcattctg
     4321 ccctacaact gacagaggcc gcgcgcccgg tgctgcgcgg cgaatcctct ttacaacttg
     4381 ccgtgccgcg tatcgtggcg ctcaaaccga aagcgatgca gaaatcgttc ggcggcaact
     4441 atgatcgcaa actgttcgcc aaattacgca aactgcgtaa atcgattgcc gatgaaagta
     4501 atgtcccgcc gtacgtggtg tttaacgacg caaccttgat tgagatggct gaacagatgc
     4561 cgatcaccgc cagcgaaatg ctcagcgtta acggcgttgg gatgcgcaag ctggaacgct
     4621 ttggtaaacc gtttatggcg ctgattcgtg cgcatgtcga tggcgacgac gaagagtagt
     4681 cagcagcata aaaaagtgcc aggatgaaga ctccgtaaac gtttcccccg cgagtcaaat
     4741 gtatgttgat gttatttctc accgtcgcca tggtgcacat tgtggcgctt atgagtcccg
     4801 gtcccgattt cttttttgtc tctcagaccg ctgtcagtcg ttcccgtaaa gaagcgatga
     4861 tgggcgtgct gggtattacc tgcggcgtta tggtttgggc agggattgcg ttgcttggcc
     4921 tgcatttgat tatcgaaaaa atggcctggc tgcatacgct gattatggtt ggcggtggcc
     4981 tgtatctctg ctggatgggt taccagatgc tgcgcggcgc gctgaaaaaa gaggcggttt
     5041 ctgcacctgc gccacaggtc gagctggcga aaagtgggcg cagtttcctg aaaggtttac
     5101 tgaccaatct cgctaatccg aaagcgatta tctactttgg ctcggtgttc tcattgtttg
     5161 tcggtgataa cgttggcact accgcgcgct ggggcatttt cgcgttgatt attgtcgaaa
     5221 cgctggcgtg gtttactgtg gtggccagcc tgtttgccct gccgcaaatg cgccgtggtt
     5281 atcaacgtct ggcgaagtgg attgatggtt ttgccggggc gttatttgcc ggatttggca
     5341 ttcatttgat tatttcgcgg taatgccaga cgcgtcttca gagtaagtcg gataaggcgt
     5401 ttacgccgca tccgacatta tttttcacgc atgcctcgcc gatgctaaca gcgctcccac
     5461 cagcataaac aacgagccga aaatcttatt cagcgccttc atctgctttg gtcctttaat
     5521 ccatagagca atccgttgag caagggtggc gtaaccgatc atcacaataa tatcgaccac
     5581 aatagtggtg acaccgagca cgatatactg catcagttgc ggctgttgcg gcatgatgaa
     5641 ttgcggaaat agcgccgcca gaaacacaat acttttggga ttggtgagat tcacaaaaac
     5701 tgcgcgctgg aacaaatgtc gacgcgattg agtagaggcc agcgatttaa gatcaattgc
     5761 gccagcggcg cgccattgct gtattcccag ccaaatcaag taagccgcgc ctgcccactt
     5821 caacacttca aacgcaatca ctgagcggga aaatagcgtc cccaacccca cgccaaccag
     5881 cacaatatga atcgccagtc cggtctgaag cccggcaata gacgccaccg cgccgcgata
     5941 accgtggttg agcgaggtgg tcatagtgtt gattgcacca gagcctggcg acaggcttaa
     6001 aatgattgat gtcagtaggt aggcaaacca ccattctaag gtcatgatga actcccggtg
     6061 tgtctatttt tgtgccacaa tacgctactg tcgcagcgtt gtgtcaggca cgctaaaaaa
     6121 aacgatttta cgtggtttaa gaggcagatt acccgatgtt tcagcagcaa aaagactggg
     6181 aaacaagaga aaacgcgttt gctgctttta ccatgggacc gctgactgat ttctggcgtc
     6241 agcgtgatga agcagagttt actggtgtgg atgacattcc ggtgcgcttt gtccgttttc
     6301 gcgcacagca ccatgaccgg gtggtagtca tctgcccggg gcgtattgag agttacgtaa
     6361 aatatgcgga actggcctac gatttgttcc atttggggtt tgatgtctta atcatcgacc
     6421 atcgcgggca gggacgttcc ggtcgcctgt tagccgatcc gcatctcggg catgttaatc
     6481 gctttaatga ttatgttgat gatctggcgg cattctggca acaggaggtc cagcccggtc
     6541 cgtggcgtaa acgctatata ctggcacatt cgatgggcgg tgcgatctcc acattatttc
     6601 tgcaacgcca tccaggtgta tgtgacgcca ttgcgctaac tgcgccaatg tttgggatcg
     6661 tgattcgtat gccgtcattt atggcacggc agatcctcaa ctgggccgaa gcgcatccac
     6721 gtttccgtga tggctatgca ataggcaccg ggcgctggcg cgcgttgccg tttgctatca
     6781 acgtactgac ccacagcaga cagcgatatc gacgtaactt acgcttctat gctgatgacc
     6841 caacgattcg cgtcggtggg ccgacctacc attgggtacg cgaaagtatt ctggctggcg
     6901 aacaggtgtt agccggtgcg ggtgatgacg ccacgccaac gcttctcttg caggctgaag
     6961 aggaacgcgt ggtggataac cgcattcatg accgtttttg tgaactccgc accgccgcgg
     7021 gccatcctgt cgaaggggga cgaccgctgg taattaaagg tgcgtaccat gagatccttt
     7081 ttgaaaagga cgcaatgcgc tcagttgcgc tccacgccat cgttgatttt ttcaacaggc
     7141 ataactcacc cagcggaaac cgctctacag aggtttaaat ttcttatgta ccaggttgtt
     7201 gcgtctgatt tagatggcac gttactttct cccgaccata cattatcccc ttacgccaaa
     7261 gaaaccctga agctgctcac cgcgcgcggc atcaactttg tgtttgcgac cggtcgtcac
     7321 cacgttgatg tggggcaaat tcgcgataat ctggagatta agtcttacat gattacctcc
     7381 aatggtgcgc gcgttcacga tctggatggt aatctgattt ttgctcataa cctggatcgc
     7441 gacattgcca gcgatctgtt tggcgtagtc aacgacaatc cggacatcat tactaacgtt
     7501 tatcgcgacg acgaatggtt tatgaatcgc catcgcccgg aagagatgcg cttttttaaa
     7561 gaagcggtgt tcaaatatgc gctgtatgag cctggattac tggagccgga aggcgtcagc
     7621 aaagtgttct tcacctgcga ttcccatgaa caactgctgc cgctggagca ggcgattaac
     7681 gctcgttggg gcgatcgcgt caacgtcagt ttctctacct taacctgtct ggaagtgatg
     7741 gcgggcggcg tttcaaaagg ccatgcgctg gaagcggtgg cgaagaaact gggctacagc
     7801 ctgaaggatt gtattgcgtt tggtgacggg atgaacgatg ccgaaatgct gtcgatggcg
     7861 gggaaaggct gcattatggg cagtgcgcac cagcgtctga aagaccttca tcccgagctg
     7921 gaagtgattg gtactaatgc cgacgacgcg gtgccgcatt atctgcgtaa actctattta
     7981 tcgtaatcgt tctttatttg gtcagttgtc aacctgatac ttcgctacaa tggatactcg
     8041 ttaatcaaag agttttccat tgtggcgcta cttatcatca ccacgattct gtgggccttc
     8101 tcctttagct tttatggcga gtaccttgcg gggcacgtcg atagctattt tgcggtgctg
     8161 gtgcgcgttg gcctggcggc actcgttttt ctgccgtttc tgcgtacccg tggcaatagc
     8221 ctgaaaacag tcggcctgta tatgctggtg ggcgcgatgc agctcggcgt gatgtatatg
     8281 ctgagtttcc gcgcttatct ctacctgacg gtttccgaac tgctgctgtt caccgtactg
     8341 acgccgctct acatcacgct gatttatgac atcatgagta agcgccgtct gcgctgggga
     8401 tatgctttta gtgccttgtt ggcggtgatt ggtgccggga ttattcgcta tgatcaggtc
     8461 accgaccatt tctggactgg cttgttgctg gtgcaactct ccaatatcac ttttgccatc
     8521 ggcatggtgg gttacaaacg cctgatggaa actcgcccga tgccgcagca taacgccttt
     8581 gcgtggttct atcttggcgc gttcctggtg gcggtaattg catggttctt gctgggaaat
     8641 gcgcagaaaa tgccgcaaac cacgttgcaa tggggcattc tggtgtttct tggcgtggtg
     8701 gcttccggga ttggctactt tatgtggaac tacggcgcaa cccaagtaga cgccggaacg
     8761 ctgggcatta tgaataatat gcacgttccg gcagggctgc tggttaacct ggctatctgg
     8821 caccaacagc cgcactggcc aacgtttatt acaggcgcgc tggtgatcct ggcctcactg
     8881 tgggtgcatc gtaagtgggt cgctccgcgc tcttcacaaa cggcagatga tcgcaggcgt
     8941 gattgcgcgc tgagcgaata aacgcttccg taactggctg acgctgctcg ccatcgcgca
     9001 cggcggcgta cagtcggctc cacaagcctt cgcccagggt tttggtcacc accagaccct
     9061 ggcgctcaaa actctctact acccaatgtg gtagcgcggc aatacccatc cgcgcggcaa
     9121 ccatctgaat caacaataag gtgttatcga cgcttttcag cgacgggcta acgcctgcgg
     9181 gctggagaaa atgccgccag acatccagtc ggctacgctg caccggataa atcaacagcg
     9241 tttcgctggc gagatcttcc ggcgtgattc gcgttttcgc cgccagtgga tggtcaggtg
     9301 ctaacaccag acgcacttca tagtcgaaca tcggcgaata atgcaggcca ctacgcggca
     9361 gaatatcgga ggtcatcacc agatccagct cgccctgctg caacgcaggc tgcgggtcaa
     9421 acgttacgcc cgatttaaaa tccatctcca cctgcggcca gttcttatgg aaattttcta
     9481 atgcgggcgt cagccactga atacagctat ggcactcaat ggcaatgcgc aggcgcgtct
     9541 gttgcggttc attgcaggct tgcagcgcct ggctaatttg cggcagcacc tggtttgcca
     9601 gttgcaacag gatttccccc tgtggagtaa agcgtagcgg ctggctctta cgcacaaata
     9661 gccggaagcc aaggcgttgt tccagatcgc taaactggtg agacagggcg gattgcgttt
     9721 gatgcaacgt cgccgcagcg gctgcgagag agccgcagtt ccgcaacgct tgtagcgttt
     9781 tcaggtgttt tacttcgatc atgaaagtcc ttcacttcgg catgaataat ttgcgcttga
     9841 ggaatataca gtaaccgcca attatggatg tgtaaacatc tggacggcta aaatccttcg
     9901 tcttttaaat ttatggtgcg ttggctgcgt ttctccaccc cggtcactta cttcagtaag
     9961 ctcccgggga tgaataaact tgccgccttc cctaaattca aaatccatag gatttacata
    10021 taattagagg aagaaaaatg acaatattga atcacaccct cggtttccct cgcgttggcc
    10081 tgcgtcgcga gctgaaaaaa gcgcaagaga gttattgggc ggggaactcc acgcgtgaag
    10141 aactgctggc ggtagggcgt gaattgcgtg ctcgtcactg ggatcaacaa aagcaagcgg
    10201 gtatcgacct gctgccggtg ggcgattttg cctggtacga tcatgtactg accaccagtc
    10261 tgctgctggg taacgttccg gcgcgtcatc agaacaaaga tggttcggta gatatcgaca
    10321 ccctgttccg tattggtcgt ggacgtgcgc cgactggcga acctgcggcg gcagcggaaa
    10381 tgaccaaatg gtttaacacc aactatcact acatggtgcc ggagttcgtt aaaggccaac
    10441 agttcaaact gacctggacg cagctgctgg acgaagtgga cgaggcgctg gcgctgggcc
    10501 acaaggtgaa acctgtgctg ctggggccga ttacctggct gtggctgggg aaagtgaaag
    10561 gtgaacagtt tgaccgcctg agcctgctga acgacattct gccggtttat cagcaagtgc
    10621 tggcagaact ggcgaaacgt ggcatcgagt gggtacagat tgatgaaccc gcgctggtac
    10681 tggaactacc acaggcgtgg ctggacgcat acaaacccgc ttacgacgcg ctccagggac
    10741 aggtgaaact gctgctgacc acctattttg aaggcgtaac gccaaatctc gacacgatta
    10801 ctgcgctgcc tgttcagggg ctgcatgtgg accttgtaca tggtaaagat gacgttgttg
    10861 aactgcacaa gcgcctgcct tctgactggc tgctgtctgc gggtctgatc aatggtcgta
    10921 acgtctggcg cgccgatctt actgagaaat atgtgcaaat taaggacatt gtcggcaaac
    10981 gtgatttgtg ggtggcatct tcctgctcgt tgctgcacag ccccatcgac ctgagcgtgg
    11041 aaacgcgtct tgatgcagaa gtgaaaagct ggtttgcctt cgccctacaa aaatgccatg
    11101 aactggcact gctgcgcgat gcgctgaaca gtggtgacac ggcagctctg gcagagtgga
    11161 gcgccccaat tcaggcgcgt cgtcactcta ctcgtgtaca taatccggcg gtagaaaagc
    11221 gtctggcggc gatcaccgcc caggacagcc agcgtgcgaa tgtctatgaa gtgcgtgctg
    11281 aagcccagcg tgcgcgtttt aaactgccag cgtggccgac caccacgatt ggttccttcc
    11341 cgcaaaccac ggaaattcgt accctgcgtc tggatttcaa aaagggcaat ctcgacgcca
    11401 ataactaccg cacgggcatt gcggaacata tcaggcaggc cattgttgaa caggaacgtt
    11461 tgggactgga tgtgctggta catggcgagg ccgagcgtaa tgacatggtg gaatactttg
    11521 gcgagcacct cgacgggttt gtctttacgc aaaacggttg ggtacagagc tacggttccc
    11581 gctgcgtgaa gccaccgatt gttattggtg acgttagtcg cccggcaccg attaccgtgg
    11641 agtgggcgaa gtatgcgcaa tctatgactg ataaaccggt gaaagggatg ctgactggcc
    11701 cggtgaccat tctctgctgg tcgttcccgc gtgaagatgt cagccgtgaa accatcgcca
    11761 aacaaattgc gctggcgctg cgtgatgaag tggcggacct ggaagccgca ggaattggca
    11821 tcatccagat tgatgaaccg gcgctgcgcg aaggtttacc gctgcgtcgt agcgactggg
    11881 atgcatatct ccagtggggc gtggaggcct tccgtatcaa cgccgccgtg gcgaaagatg
    11941 acacacaaat ccacactcac atgtgttatt gcgagttcaa cgacatcatg gattcgattg
    12001 cggcactgga cgcagacgtc atcaccatcg aaacctcgcg ttctgacatg gagttgctgg
    12061 agtcgttcga agagtttgat tatccgaatg aaatcggtcc aggcgtctat gacattcact
    12121 cgccaaacgt accgagcgtg gaatggattg aagccttgct gaagaaagcg gcaaaacgca
    12181 ttccggcaga gcgcctgtgg gtcaacccgg actgtggcct gaaaacgcgc ggctggccag
    12241 aaacccgcgc ggcactggcg aacatggtgc aggcggcgca gaacttgcgt cgggggtaaa
    12301 atccaaaccg ggtggtaata ccacccggtc ttttctcatt acagcgactt cttcccacca
    12361 tactgcttaa accattccag catacgctgc cagccatctt ctgcagatgc ggcatgatag
    12421 ctcgggcgat aatcagcgtt gaatgcatgc ccggcgtccg ggtacacgat aatctctgct
    12481 ttcgcattag cagcccgcag cgcctggcgc atggtttcaa cgctctcctg cggaatactg
    12541 ttatccagac cgccatataa gccgagaacc ggcgcgttaa gatcggttgc gatatcaaca
    12601 ggttgtttcg gtgaattcag cgacttatcg cccgtcagtt tgccgtacca cgccactgcg
    12661 gcttttagct gtggattatg cgcggcatac agccaagtga tacgtccacc ccagcagaat
    12721 ccggtgatca ttaaacgatg aacatcgccg ccgttgcggg aagcccaact ggcgacatga
    12781 tcgagatcgg ccagcacctg cgagtcaggc acttttgcta ccagaccgct aagcaacgtg
    12841 gggatatcgg caaaatcatt cggatcgcct tcgcggaagt aaagttcagg tgcgatagcc
    12901 agatacccct ccagcgccag acggcgacaa atgtcgcgga tatgttcatg cacgccaaaa
    12961 atttcctgca ctacaatgac cactggcagt gggccatcgc tttgctttgg tctggcatgg
    13021 taagcaggca tgttatcccc ttgtgaaggg atagaggtga agcccgccac aatcgcgtcg
    13081 tccggggtct gaacgatggt cgaagcgaga ggcgatgcag caggtgcaaa tccagattgt
    13141 tgtgttgttg ccatggtatt ctccgtaccc ttataaaaat gttgcgcaat gttaactata
    13201 gtcagcatgc aacaaatcac attgcctgaa tcggctcatc ttttatgcag tcctgcagaa
    13261 tgaagagtga tttatgtgat ttgcatcacc tttggtgggt aaatttatgc aacgcatttg
    13321 cgtcatggtg atgagtatca cgaaaaaatg ttaaaccctt cggtaaagtg tctttttgct
    13381 tcttctgact aaaccgattc acagaggagt tgtatatgtc caagtctgat gtttttcatc
    13441 tcggcctcac taaaaacgat ttacaagggg ctacgcttgc catcgtccct ggcgacccgg
    13501 atcgtgtgga aaagatcgcc gcgctgatgg ataagccggt taagctggca tctcaccgcg
    13561 aattcactac ctggcgtgca gagctggatg gtaaacctgt tatcgtctgc tctaccggta
    13621 tcggcggccc gtctacctct attgctgttg aagagctggc acagctgggc attcgcacct
    13681 tcctgcgtat cggtacaacg ggcgctattc agccgcatat taatgtgggt gatgtcctgg
    13741 ttaccacggc gtctgtccgt ctggatggcg cgagcctgca cttcgcaccg ctggaattcc
    13801 cggctgtcgc tgatttcgaa tgtacgactg cgctggttga agctgcgaaa tccattggcg
    13861 cgacaactca cgttggcgtg acagcttctt ctgatacctt ctacccaggt caggaacgtt
    13921 acgatactta ctctggtcgc gtagttcgtc actttaaagg ttctatggaa gagtggcagg
    13981 cgatgggcgt aatgaactat gaaatggaat ctgcaaccct gctgaccatg tgtgcaagtc
    14041 agggcctgcg tgccggtatg gtagcgggtg ttatcgttaa ccgcacccag caagagatcc
    14101 cgaatgctga gacgatgaaa caaactgaaa gccaagcggt gaaaatcgtg gtggaagcgg
    14161 cgcgtcgtct gctgtaattc tcttctcctg tctgaaggcc gacgcgttcg gccttttgta
    14221 tttttgcgta gcgcctcgca ggaaatgcct ttccaactgg acgtttgtac agcacaattc
    14281 tattttgtgc gggtaagttg ttgcgtcagg aggcgttgtg gatttctcaa tcatggttta
    14341 cgcagttatt gcgttggtgg gtgtggcaat tggctggctg tttgccagtt atcaacatgc
    14401 gcagcaaaaa gccgagcaat tagctgaacg tgaagagatg gtcgcggagt taagcgcggc
    14461 aaaacaacaa attacccaaa gcgagcactg gcgtgcagag tgcgagttac tcaataacga
    14521 agtgcgcagc ctgcaaagta ttaacacctc tctggaggcc gatctgcgtg aagtaaccac
    14581 gcggatggaa gccgcacagc aacatgctga cgataaaatt cgccagatga ttaacagcga
    14641 gcagcgcctc agtgagcagt ttgaaaacct cgccaaccgt atttttgagc acagcaatcg
    14701 ccgggttgat gagcaaaacc gtcagagtct gaacagcctg ttgtcgccgc tacgtgaaca
    14761 actggacggt ttccgccgtc aggttcagga cagcttcggt aaagaagcac aagaacgcca
    14821 taccctgacc cacgaaattc gcaatctcca gcaactcaac gcgcaaatgg cccaggaagc
    14881 gatcaacctg acgcgcgcgc tgaaaggcga caataaaacc cagggcaact ggggcgaggt
    14941 agtattgacg cgggtgctgg aggcttccgg tctgcgtgaa gggtatgaat atgaaaccca
    15001 ggtcagcatc gaaaatgacg cccgctcgcg gatgcagccg gatgtcatcg tgcggctgcc
    15061 gcagggaaaa gatgtggtga tcgacgccaa aatgacgctg gtcgcctatg aacgctattt
    15121 taacgccgaa gacgactaca cccgcgaaag cgcgctacag gaacatatcg cgtcggtgcg
    15181 taaccatatc cgtttgctgg gacgcaaaga ttatcaacag ctgccggggc tgcgaactct
    15241 ggattacgtg ctgatgttta ttcccgttga acccgctttt ttactggcgc ttgaccgcca
    15301 gccggagctg atcaccgaag cgttgaaaaa caatatcatg ctggttagcc cgactacgct
    15361 gctggtggcg ctgcgcacta tcgccaacct gtggcgttat gagcatcaaa gccgcaacgc
    15421 ccagcaaatc gccgatcgtg ccagcaagct gtacgacaag atgcgtttgt tcatcgatga
    15481 catgtccgcg attggtcaaa gtctcgacaa agcgcaggat aactatcggc aggcaatgaa
    15541 aaaactctct tcagggcgcg gaaatgtgct ggcgcaggca gaagcgtttc gcggtttagg
    15601 agtagaaatt aaacgcgaga ttaatccgga tttggctgaa caggcggtga gccaggatga
    15661 agagtatcga cttcggtcgg ttccggagca gccgaatgat gaagcttatc aacgcgatga
    15721 tgaatataat cagcagtcgc gctagcccat tgggagtagt taagccgggt agaaatctag
    15781 ggcatcgacg cccaatctgt tacacttctg gaacaatttt ttgatgagca ggcattgaga
    15841 tggtggataa gtcacaagaa acgacgcact ttggttttca gaccgtcgcg aaggaacaaa
    15901 aagcggatat ggtcgcccac gttttccatt ccgtggcatc aaaatacgat gtcatgaatg
    15961 atttgatgtc atttggtatt catcgtttgt ggaagcgatt cacgattgat tgcagcggcg
    16021 tacgccgtgg gcagaccgtg ctggatctgg ctggtggcac cggcgacctg acagcgaaat
    16081 tctcccgcct ggtcggagaa actggcaaag tggtccttgc tgatatcaat gaatccatgc
    16141 tcaaaatggg ccgcgagaag ctgcgtaata tcggtgtgat tggcaacgtt gagtatgttc
    16201 aggcgaacgc tgaggcgctg ccgttcccgg ataacacctt tgattgcatc accatttcgt
    16261 ttggtctgcg taacgtcacc gacaaagata aagcactgcg ttcaatgtat cgcgtgctga
    16321 aacccggcgg ccgcctgctg gtgcttgagt tctcgaagcc aattatcgag ccgctgagca
    16381 aagcctatga tgcatactcc ttccatgtgc tgccgcgtat tggctcactg gtcgcgaacg
    16441 acgccgacag ctaccgttat ctggcagaat ccatccgtat gcatcccgat caggataccc
    16501 tgaaagccat gatgcaggat gccggattcg aaagtgtcga ctactacaat ctgacggcag
    16561 gggttgtggc gctgcatcgt ggttataagt tctgacagga gaccggaaat gccttttaaa
    16621 cctttagtga cggcaggaat tgaaagtctg ctcaacacct tcctgtatcg ctcacccgcg
    16681 ctgaaaacgg cccgctcgcg tctgttgggc aaagtgctgc gcgtggaggt aaaaggcttt
    16741 tcgacgtcat tgattctggt gttcagcgaa cgccaggttg atgtactggg cgaatgggca
    16801 ggcgatgctg actgcaccgt tatcgcctac gccagtgtgt tgccgaaact tcgcgatcgc
    16861 cagcagcttg ccgccctgat tcgcagtggt gagctggaag tgcagggcga tattcaggtg
    16921 gtgcaaaact tcgttgcgct ggcaggtctg gcagagttcg accctgcgga actgctggcc
    16981 ccttataccg gtgatatcgc cgctgaagga atcagcaaag ccatgcgcgg aggcgcaaag
    17041 ttcctgcatc acggcattaa gcgccagcaa cgttatgtgg cggaagccat tactgaagag
    17101 tggcgtatgg cacccggtcc gcttgaagtg gcctggtttg cggaagagac ggctgccgtc
    17161 gagcgtgctg ttgatgccct gatcaaacgg ctggaaaaac tggaggctaa atgacgccag
    17221 gtgaagtacg gcgcctatat ttcatcattc gcactttttt aagctacgga cttgatgaac
    17281 tgatccccaa aatgcgtatc accctgccgc tacggctatg gcgatactca ttattctgga
    17341 tgccaaatcg gcataaagac aaacttttag gtgagcgact acgactggcc ctgcaagaac
    17401 tggggccggt atggatcaag ttcgggcaaa tgttatcaac ccgccgcgat ctttttccgc
    17461 cgcatattgc cgatcagctg gcgttattgc aggacaaagt tgctccgttt gatggcaagc
    17521 tggcgaagca gcagattgaa gctgcaatgg gcggcttgcc ggtagaagcg tggtttgacg
    17581 attttgaaat caagccactg gcttctgctt ctatcgccca ggttcatacc gcgcgattga
    17641 aatcgaatgg taaagaggtg gtgattaaag tcatccgccc ggatattttg ccggtcatta
    17701 aagcagacct gaaacttatc taccgtctgg ctcgctgggt gccgcgtttg ctgccggatg
    17761 gtcgccgtct acgcccaacc gaagtggtgc gcgagtacga aaagaccttg attgatgaac
    17821 tgaatttgct gcgggaatct gccaacgcca ttcagcttcg gcgaaatttt gaagacagcc
    17881 cgatgctcta catcccggag gtttaccctg actattgtag tgaagggatg atggtgatgg
    17941 agcgtattta cggcattccg gtgtctgatg ttgcggcgct ggagaaaaac ggcaccaaca
    18001 tgaaattgct ggcggaacgc ggcgtgcagg tgttcttcac tcaggtcttt cgcgacagct
    18061 ttttccatgc cgatatgcac cctggcaaca tcttcgtaag ctatgaacac ccggaaaacc
    18121 cgaaatatat cggcattgat tgcgggattg ttggctcgct aaacaaagaa gataaacgct
    18181 atctggcaga aaactttatt gccttcttta atcgcgacta tcgcaaagtg gcagagctac
    18241 acgtcgattc tggctgggtg ccaccagata ccaacgttga agagttcgaa tttgctattc
    18301 gtacggtctg tgaacctatc tttgagaaac cgctggccga aatttcgttt ggacatgtac
    18361 tgttaaatct gtttaatacg gcgcgtcgct tcaatatgga agtgcagccg caactggtgt
    18421 tactccagaa aaccctgctc tacgtcgaag gggtaggacg ccagctttat ccgcaactcg
    18481 atttatggaa aacggcgaag cctttcctgg agtcgtggat taaagatcag gtcggtattc
    18541 ctgcgctggt gagagcattt aaagaaaaag cgccgttctg ggtcgaaaaa atgccagaac
    18601 tgcctgaatt ggtttacgac agtttgcgcc agggcaagta tttacagcac agtgttgata
    18661 agattgcccg cgagcttcag tcaaatcatg tacgtcaggg acaatcgcgt tattttctcg
    18721 gaattggcgc tacgttagta ttaagtggca cattcttgtt ggtcagccga cctgaatggg
    18781 ggctgatgcc cggctggtta atggcaggtg gtctgatcgc ctggtttgtc ggttggcgca
    18841 aaacacgctg attttttcat cgctcaaggc gggccgtgta acgtataatg cggctttgtt
    18901 taatcatcat ctaccacaga ggaacatgta tgggtggtat cagtatttgg cagttattga
    18961 ttattgccgt catcgttgta ctgctttttg gcaccaaaaa gctcggctcc atcggttccg
    19021 atcttggtgc gtcgatcaaa ggctttaaaa aagcaatgag cgatgatgaa ccaaagcagg
    19081 ataaaaccag tcaggatgct gattttactg cgaaaactat cgccgataag caggcggata
    19141 cgaatcagga acaggctaaa acagaagacg cgaagcgcca cgataaagag caggtgtaat
    19201 ccgtgtttga tatcggtttt agcgaactgc tattggtgtt catcatcggc ctcgtcgttc
    19261 tggggccgca acgactgcct gtggcggtaa aaacggtagc gggctggatt cgcgcgttgc
    19321 gttcactggc gacaacggtg cagaacgaac tgacccagga gttaaaactc caggagtttc
    19381 aggacagtct gaaaaaggtt gaaaaggcga gcctcactaa cctgacgccc gaactgaaag
    19441 cgtcgatgga tgaactacgc caggccgcgg agtcgatgaa gcgttcctac gttgcaaacg
    19501 atcctgaaaa ggcgagcgat gaagcgcaca ccatccataa cccggtggtg aaagataatg
    19561 aagctgcgca tgagggtgta acgcctgccg ctgcacaaac gcaggccagt tcgccggaac
    19621 agaagccaga aaccacgcca gagccggtgg taaaacctgc tgcggacgct gaaccgaaaa
    19681 ccgctgcacc ttccccttcg tcgagtgata aaccgtaaac atgtctgtag aagatactca
    19741 accgcttatc acgcatctga ttgagctgcg taagcgtctg ctgaactgca ttatcgcggt
    19801 gatcgtgata ttcctgtgtc tggtctattt cgccaatgac atctatcacc tggtatccgc
    19861 gccattgatc aagcagttgc cgcaaggttc aacgatgatc gccaccgacg tggcctcgcc
    19921 gttctttacg ccgatcaagc tgacctttat ggtgtcgctg attctgtcag cgccggtgat
    19981 tctctatcag gtgtgggcat ttatcgcccc agcgctgtat aagcatgaac gtcgcctggt
    20041 ggtgccgctg ctggtttcca gctctctgct attttatatc ggcatggcgt tcgcctactt
    20101 tgtggtcttt ccgctggcat ttggcttcct tgccaatacc gcgccggaag gggtgcaggt
    20161 atccaccgac atcgccagct atttaagctt cgttatggcg ctgtttatgg cgtttggtgt
    20221 ctcctttgaa gtgccggtgg caattgtgct gctgtgctgg atggggatta cctcgccaga
    20281 agacttacgc aaaaaacgcc cgtatgtgct ggttggtgca ttcgttgtcg ggatgttgct
    20341 gacgccgccg gatgtcttct cgcaaacgct gttggcgatc ccgatgtact gtctgtttga
    20401 aatcggtgtc ttcttctcac gcttttacgt tggtaaaggg cgaaatcggg aagaggaaaa
    20461 cgacgctgaa gcagaaagcg aaaaaactga agaataaatt caaccgcccg tcagggcggt
    20521 tgtcatatgg agtacaggat gtttgatatc ggcgttaatt tgaccagttc gcaatttgcg
    20581 aaagaccgtg atgatgttgt agcgcgcgct tttgacgcgg gagttaatgg gctactcatc
    20641 accggcacta acctgcgtga aagccagcag gcgcaaaagc tggcgcgtca gtattcgtcc
    20701 tgttggtcaa cggcgggcgt acatcctcac gacagcagcc agtggcaagc tgcgactgaa
    20761 gaagcgatta ttgagctggc cgcgcagcca gaagtggtgg cgattggtga atgtggtctc
    20821 gactttaacc gcaacttttc gacgccggaa gagcaggaac gcgcttttgt tgcccagcta
    20881 cgcattgccg cagatttaaa catgccggta tttatgcact gtcgcgatgc ccacgagcgg
    20941 tttatgacat tactggagcc gtggctggat aaactgcctg gtgcggttct tcattgcttt
    21001 accggcacac gcgaagagat gcaggcgtgc gtggcgcatg gaatttatat cggcattacc
    21061 ggttgggttt gcgatgaacg acgcggactg gagctgcggg aacttttgcc gttgattccg
    21121 gcggaaaaat tactgatcga aactgatgcg ccgtatctgc tccctcgcga tctcacgcca
    21181 aagccatcat cccggcgcaa cgagccagcc catctgcccc atattttgca acgtattgcg
    21241 cactggcgtg gagaagatgc cgcatggctg gctgccacca cggatgctaa tgtcaaaaca
    21301 ctgtttggga ttgcgtttta gagtttgcgg aactcggtgt tcttcacact gtgcttaatc
    21361 tctttattaa taagattaag caatagcatg gagcgagcct caccatcggg ttcggtgaaa
    21421 atggcctgaa agccttcgaa cgcgccttcg gtaataatca ccttatcacc cggataaggg
    21481 gttgccggat cgacaatgtc tttcggttta tataccgata gctgatgaat caccgccgat
    21541 gggactatcg ctggcgacgc gccaaagcgc acgaagtggc tgacaccgcg ggtcgcgttg
    21601 atagtcgtgg tatgaatcac ttctggatca aattccacaa acaggtagtt ggggaacaat
    21661 ggctcactga ctgcagtacg tttgccacgc acgatttttt ccagggtgat catcggtgcc
    21721 aggcaattca cagcctgtct ttcgaggtgt tcctgggcac gttgaagttg cccgcgcttg
    21781 cagtacagta aataccagga ttgcataatg actcttatcc gtttaatcgg ggcgcaagga
    21841 tagcaaaagc tttacgctaa gttaattata ttccccggtt tgcgttatac cgtcagagtt
    21901 cacgctaatt taacaaattt acagcatcgc aaagatgaac gccgtataat gggcgcagat
    21961 taagaggcta caatggacgc catgaaatat aacgatttac gcgacttctt gacgttgctt
    22021 gaacagcagg gtgagctaaa acgtatcacg ctcccggtgg acccgcatct ggaaatcact
    22081 gaaattgctg accgcacgct gcgtgctggt gggcctgcgc tgttgtttga aaaccctaaa
    22141 gggtactcaa tgccggtgct gtgcaacttg ttcggtacgc caaagcgcgt agcgatgggt
    22201 atgggccagg aagatgtttc agcactgcgt gaagtcggta aattattagc atttctgaaa
    22261 gaaccagagc cgccaaaagg ttttcgcgat ctgtttgata agctgccgca gtttaagcag
    22321 gtgttaaaca tgccgacaaa gcgactgcgc ggtgcaccct gccaacaaaa aatcgtctct
    22381 ggcgatgacg tcgatctcaa ccgtattccc attatgacct gttggccgga agatgccgcg
    22441 ccgctgatta catgggggct aaccgttaca cgtggccctc ataaagagcg acagaatctg
    22501 ggcatttatc gccagcaact gattggtaaa aacaagctga ttatgcgttg gctgtcgcat
    22561 cgcggcggcg cgctggatta tcaggagtgg tgtgcggcgc atccaggtga acgtttcccg
    22621 atctctgtgg cgttgggcgc tgatccggca accattctcg gtgcagtcac accagtacca
    22681 gatactttgt cggaatacgc ctttgccgga ttgctacgtg gcaccaaaac cgaagtagtg
    22741 aagtgtattt ccaatgatct cgaagtgccc gccagtgcgg agattgtgct ggaagggtat
    22801 atcgaacaag gcgaaatggc gccagaagga ccgtatggtg accacactgg ttactataac
    22861 gaagtcgata gtttcccggt atttaccgtg acgcatatta cccagcgtga agatgcgatt
    22921 taccattcca cctataccgg gcgtccgcca gatgaacccg cggtactggg agtggcgttg
    22981 aacgaagtat ttgttcccat tctgcaaaag cagttcccgg aaattgtcga tttttacctg
    23041 ccgccggaag gctgctctta tcgcctggcg gtagtgacaa tcaaaaaaca gtacgccgga
    23101 cacgcgaagc gcgtcatgat gggcgtctgg tcgttcttac gccagtttat gtacactaaa
    23161 tttgtgatcg tttgcgatga tgacgttaac gcacgcgact ggaacgatgt gatttgggcg
    23221 attaccaccc gtatggaccc agcgcgggat actgttctgg tagaaaatac gcctattgat
    23281 tatctggatt ttgcctcgcc tgtctccggg ctgggttcaa aaatggggct ggatgccacg
    23341 aataaatggc cgggggaaac ccagcgtgaa tggggacgtc ccatcaaaaa agatccagat
    23401 gttgtcgcac atattgacgc catctgggat gaactggcta tttttaacaa cggtaaaagc
    23461 gcctgatgcg cgtttgtttt gccctattta tcgatccgac agagaaagcg catgacaacc
    23521 ttaagctgta aagtgacctc ggtagaagct atcacggata ccgtatatcg tgtccgcatc
    23581 gtgccagacg cggccttttc ttttcgtgct ggtcagtatt tgatggtagt gatggatgag
    23641 cgcgacaaac gtccgttctc aatggcctcg acgccggatg aaaaagggtt tatcgagctg
    23701 catattggcg cttctgaaat caacctttac gcgaaagcag tcatggaccg catcctcaaa
    23761 gatcatcaaa tcgtggtcga tattccccac ggagaagcgt ggctgcgcga tgatgaagag
    23821 cgtccgatga ttttgattgc gggcggcacc gggttctctt atgcccgctc gattttgctg
    23881 acagcgttgg cgcgtaaccc aaaccgtgat atcaccattt actggggcgg gcgtgaagag
    23941 cagcatctgt atgatctctg cgagcttgag gcgctttcgt tgaagcatcc tggtctgcaa
    24001 gtggtgccgg tggttgaaca accggaagcg ggctggcgtg ggcgtactgg caccgtgtta
    24061 acggcggtat tgcaggatca cggtacgctg gcagagcatg atatctatat tgccggacgt
    24121 tttgagatgg cgaaaattgc ccgtgacctg ttttgcagtg agcgtaatgc gcgggaagat
    24181 cgcctgtttg gcgatgcgtt tgcatttatc tgagatataa aaaaacccgc ccctgacagg
    24241 cgggaagaac ggcaactaaa actttattca gtggcattta gatctatgtc gcatccggca
    24301 ttcggcacac gatgcctgat gcgacgctgg cgcgtcttat caggcctaca ttggtgccgg
    24361 atcggtagac cggataaggc gttcacgccg catccggcaa gtggttaaac ccgctcaaac
    24421 accgtcgcaa taccctgacc cagaccgata cacatcgtcg ccagaccaaa ctgaacgtct
    24481 ttgtgttcca tcagattcag cagcgtggtg ctgatacgcg caccggaaca acccagcgga
    24541 tgacccagcg cgatcgcgcc accgttgagg ttgatcttct cgtcaatctg ctcaattagt
    24601 cccagatctt taatacatgg caggatctgc gcggcaaagg cttcattcat ttcaaacacg
    24661 ccgatatcac tgacagaaag ccccgctttt ttcagcgcca gcttcgaggc cggaaccggg
    24721 ccgtaaccca tgatcgatgg gtcacaacca acgaccgcca tcgaacgcac acgagcgcgc
    24781 ggcttaagac ctaattcatg ggcgcggctt tcactcatca ccagcatggc agctgcgcca
    24841 tcggaaagtg cagaagatgt gcccgccgtt accgtaccgt ttactggatc aaacgccgga
    24901 cgcagcgtgg cgagggcttc cacggtggtt tccgggcgaa tcacttcgtc gtaattaaac
    24961 tgcttcagga cgccgtcggc atcgtgacca ccagttggga tgatttcatt tttaaatgcg
    25021 cccgactgcg tggcggccca ggcgcgggcg tgtgaccgcg cggcaaaggc atcctgcatt
    25081 tcacggctga taccgtgcat acgcgccagc atttctgccg ttaagcccat catgcccgcc
    25141 gctttggcga cattgcggct caggccaggg tgaaaatcga cgccgtgact catcggcaca
    25201 tggcccatat gctccacgcc gccaaccaga catgcctgcg catcgccggt catgatcatt
    25261 cgtgctgcgt catgcagtgc ctgcatggat gaaccacaca agcgattgac agtaaccgcc
    25321 gggacagagt gcggcacttc tgccagcagc gccgcgttac gggcgatatt aaaaccctgc
    25381 tccagcgtct gctgcacaca cccccagtaa atatcgtcga gggccgccgc ttccagcgcc
    25441 gggttacgcg ccagcaggct acgcattaaa tgagcggaga gatcttctgc acgcacgtta
    25501 cgaaaagcac cgcccttcga acggcccatc ggggtgcgaa ttgcatcgac aatgacaacc
    25561 tgttccattg tgactcctta agccgttttc aggtcgccaa ccggacgggc tggctcaacc
    25621 gggggatagt acggttcgtt atgacgcgct ttattacgca gaccttccgg cacttcgtac
    25681 agcgggccga ggtgctgata ttgctgtgcc atatcgaggt attttgcgct gccgagggtg
    25741 tccagccagc ggaacgcgcc gccgtggaac ggagggaagc ccaggccgta gaccagcgcc
    25801 atatccgctt ctgccggagt ggcgataatg ccttcttcca gacagcgcac cacttcgttg
    25861 accatcggga tcatcatgcg ggcgataatc tcttcttcgc tgaaatcgcg cttcggctgg
    25921 ctcacttctg ccagcaggtc gtcaacagcg gcgtcttctt ctttcttcgg cttacctttg
    25981 ctgtcttctt tataatgcca gaaaccgagg ccgttcttct gaccaaagcg gttggcatca
    26041 aacagcgcgt cgatggcatc gcgataatct ttctgcatcc gctgtgggaa gcccgctgcc
    26101 atgactgcct gggcgtgatg cgcggtgtca atgcccacaa cgtccagcag atatgccgga
    26161 cccataggcc agccaaactg tttttccatc actttgtcga tcttgcggaa atccgcgccg
    26221 tcgcgcagca actggctgaa accggcgaaa tacgggaaca gcacgcggtt gacgaagaag
    26281 ccggggcaat cgttaaccac aatcggcgtt ttacccatct tgctcgccca ggcgacgact
    26341 ttcgcaatag tttcgtcgga gcttttctcg ccgcgaataa tttccaccaa cggcattcgg
    26401 tggaccgggt taaagaagtg catcccgcag aagttttccg gacgttccag cgcgttggcc
    26461 agttcgctga taggaatggt tgaagtgtta gacgccagca cggtatccgg gcgtactttc
    26521 tgctcggttt ctgccagcac ggcttttttc actttcgggt tttcaacaat cgcttctacc
    26581 acaacatcca cgcgctcaaa tccggcgtag tcgagcgttg ggtggatcgt ggagatcacg
    26641 ccagccagtt tcagaccatc gatcttgccg cgctcaagct gcttgttcag tagtttcgcg
    26701 gcttcagtca tgccgagggc taacgacttg tcgttaatat ctttcataat caccggcacg
    26761 cctttccacg cagactggta agcgatgccg ccacccataa tgcctgcgcc cagcacggcg
    26821 gcctgtttcg gggtttcaac gtctttggtg agtttcttcg ctttgccttt tacatattga
    26881 tcgttaagga aaatgccgac cagtgcgcga gcttcgttgg tatgcgccag cgggacaaag
    26941 cttttgtttt ctaggtttaa ggcttcttca cgaccaaaac gagccgcagc ttcaatggtt
    27001 tttactgcgg tgatgggggc cgggtaatgt tttcctgccg tttgtgcgac catcccttta
    27061 gcgatggtga agctcatggt ggcttcaatc ttgctcagtt ttagcggttc cagcttcggc
    27121 tgacgtttcg ctttccagtc gaggtcgccg ttaatggcct ggcgtaaaat cgccatcgcc
    27181 ccttcaatca gtttttctgc tttgactacg ccatccacca gaccgatttt cagcgcctga
    27241 tccgcgccga catctttacc ggcggcaatg atttccagcg cactgtcagc gcccagcata
    27301 cgtggcatac gtacagaacc gccaaagccc ggcatgatgc ccagtttggt ttccggcaga
    27361 ccgatgcgca gatccggcgt cgccagacgg taatcggtcg ccagcacgca ttcgcagcca
    27421 ccgcccagcg catagccatt gacggcagca atggtcggca ccggcagatc ttccaggcga
    27481 ttaaacacgc tattggcaaa gtgcagccac tgacttaact gttcttcagg aacgaggaac
    27541 agggacaaaa attcggtgat atcagcaccg acgataaagg ctgctttgtt cgaacgcagc
    27601 agcagccctt ttagatctga ttgctgttcc agcacgccga tggcctcgcc gaggctggcg
    27661 acggtcgcag tgtcgagttt attaactgaa cctggggcat caaataccag ttcggcaatg
    27721 ccatcttcca gccagtcaag gtacagggtg tcgcctttgt aaagcatgtc agtctcctga
    27781 atccgcaagg tgatctggtc gtaccagatg catcgaagtg tgtattttgt gttaaaaata
    27841 tgcaaataaa agattaaaga aatgccgatc tgatcacgct cggcagaaat cacgctctgg
    27901 atgaacgatg tgctaagatg cggagactta aggtcaaaaa aacagaaggg taaaaaatgg
    27961 aatcactggc ctcgctctat aaaaatcata tagctacatt gcaggaacga actcgcgatg
    28021 cgctgacgcg cttcaagctg gatgcgttac ttattcactc cggcgaactg ttcaacgttt
    28081 ttctcgacga tcatccctat ccgtttaaag tgaacccgca attcaaagcg tgggtgccgg
    28141 taactcaggt gccaaactgc tggctgctgg tggatggcgt gaataagccg aaactgtggt
    28201 tctatctgcc ggttgattac tggcacaacg tcgaaccgct gccgacctcc ttctggactg
    28261 aagatgtaga agtgatcgcg ctgccgaaag ccgatggcat tggtagtctg ttgcctgctg
    28321 cgcgcggcaa tatcggttat atcggtccgg tgccggaacg tgcgctgcaa ctgggtattg
    28381 aggccagcaa tatcaacccg aaaggggtta tcgactacct gcattactac cgctccttca
    28441 aaaccgagta cgagctggcc tgtatgcgtg aagcgcagaa aatggcggtc aacggtcatc
    28501 gcgcggcaga agaagcgttc cgttctggca tgagcgagtt cgatatcaat attgcctatc
    28561 tgactgcgac cggtcatcgt gataccgacg taccttacag caacattgtg gcactcaacg
    28621 aacacgctgc ggtgctgcat tacaccaaac tggatcatca ggcgtcggaa gagatgcgca
    28681 gcttcctgct ggatgccggg gccgaatata acggctatgc cgctgacctg acccgtacct
    28741 ggtcggcaaa aagtgacaac gattacgcac agctggtgaa agacgtaaat gatgaacaac
    28801 tggcgctgat cgcgaccatg aaagctggcg ttagctatgt ggattaccac atccagttcc
    28861 atcagcgcat cgccaaattg ctgcgtaaac atcaaatcat caccgatatg agtgaagagg
    28921 cgatggtcga aaacgatctt accgggccgt ttatgccgca tggtatcggc catccgctgg
    28981 gcctgcaggt gcatgacgtc gccggtttta tgcaggatga tagcggtacg cacctcgcgg
    29041 caccggcaaa atatccgtac ctgcgctgca cccgtattct ccagccgggc atggtgttaa
    29101 ccatcgaacc gggtatctac ttcattgaat cgctgctggc accgtggcgt gaagggcagt
    29161 tcagcaagca cttcaactgg cagaaaattg aagcactgaa accgttcggc ggcattcgta
    29221 tcgaagacaa cgtggtgatc cacgaaaata acgtggaaaa catgacccgg gatctgaaac
    29281 tggcgtgatg gaaagctggt taattcctgc ggcaccggtc acggtcgttg aagagatcaa
    29341 aaaaagccgc ttcattacgc tgttggcgca taccgatggc gttgaggcgg cgaaagcgtt
    29401 tgttgaatcg gtgcgggcag aacaccccga tgcccgccac cattgcgtgg cgtgggtggc
    29461 gggtgcgccg gatgattctc aacagctggg tttctctgac gacggggagc cggcgggaac
    29521 ggcaggtaaa ccgatgctcg cccagctaat gggcagcggc gttggggaaa ttaccgctgt
    29581 ggtggtgcgc tactacggcg gcatattgct tggcaccggt gggttagtga aagcgtatgg
    29641 cggcggcgtg aatcaggcgc tgcgccagct aacgacccaa cgcaagacgc cattaaccga
    29701 atatactttg caatgtgaat atagtcagtt aaccggcatt gaagcgttgc tggggcagtg
    29761 tgacggcaaa attatcaaca gtgattatca ggcattcgtt ctgctgcggg tggcgcttcc
    29821 ggcggcgaaa gtggctgaat tttccgcgaa gctggcggat tttagccgtg gttcattgca
    29881 attgttagcg attgaagaat aatccccact tcgttttgca gaactaagga agcggcagag
    29941 atgcattttc gcgccattac ccgaatcgtt ggactactgg tcatcttatt ttcagggacc
    30001 atgattatcc ctgggctggt agcactcatc taccgggatg gagcgggccg cgcttttacc
    30061 cagacctttt ttgtcgccct cgccattggc tctatgctgt ggtggccgaa ccgcaaagag
    30121 aagggcgaac tgaaatcccg tgaggggttt ctgatagtgg tgctgttctg gaccgtgctg
    30181 ggtagtgtcg gtgcgctccc ttttatcttc tcggaaagcc cgaacctcac gattaccgat
    30241 gcgttttttg aatctttctc tggcctgacc accaccgggg ccactacgct ggtggggctg
    30301 gattcgctcc ctcatgccat cctcttttat cgccagatgc tgcaatggtt tggcgggatg
    30361 gggatcatcg tgttagcggt tgcgatactg cctatcctcg gcgtgggtgg gatgcagctc
    30421 tatcgcgcag aaatgcccgg cccgctgaaa gataacaaaa tgcgcccgcg aattgcggaa
    30481 acggcgaaaa ccctgtggtt gatttatgtc ttgctgaccg tcgcctgtgc gctggcgttg
    30541 tggtttgccg gaatggatgc ctttgatgcc atcggccata gctttgcgac tatcgctatt
    30601 ggcggcttct cgacacatga tgccagtatc ggttatttcg atagcccgac tattaacact
    30661 atcattgcta tcttcctgct gatctccggc tgtaactacg gtctgcactt ttcactgtta
    30721 agtgggcgta gtctgaaggt ttattggcgc gatccggaat ttcgcatgtt tatcggcgta
    30781 cagtttacgc tggtggttat ttgtaccctc gtactgtggt ttcataatgt ctacagttcg
    30841 gcgctgatga caattaacca ggcgtttttc caggtggtgt cgatggcgac aaccgccggg
    30901 tttacgactg acagcattgc ccgctggccg ctctttttgc cggtactgct tttatgttca
    30961 gcttttatcg gcggttgtgc cgggtcaacg ggcggtggcc tgaaagtgat ccgcatcctg
    31021 ctgctgttta agcaggggaa ccgtgaactg aaacgactgg tgcatccgaa cgccgtctat
    31081 agcattaagc tggggaatcg cgcactgccg gaacgtatcc tcgaagccgt ttggggattt
    31141 ttctccgcct atgcattggt gtttattgtc agtatgctgg cgattatcgc cacgggcgtg
    31201 gatgactttt ctgcctttgc ctcggttgtt gcgacattga ataacctggg gccagggctt
    31261 ggcgtggttg ctgataactt taccagtatg aacccggtgg ctaaatggat cctgattgcc
    31321 aacatgctgt ttggtcgtct cgaggtcttt acattgctgg tgctctttac cccgactttc
    31381 tggcgtgaat gatggagtaa tacgtgaaaa cattaattct tttctcaaca agggacggac
    31441 aaacgcgcga gattgcctcc tacttggctt cggaactgaa agaactgggg atccaggcgg
    31501 atgtcgccaa tgtgcaccgc attgaagaac cacagtggga aaactacgac cgtgtggtca
    31561 ttggtgcttc tattcgctat ggtcactacc actctgcgtt ccaggaattt gtcaaaaagc
    31621 atgcgacgcg gctgaattcg atgccgagcg cgttttactc cgtaaacctg gtggcgcgta
    31681 aaccggagaa gcgcactcca cagaccaaca gctacgcgcg gaagtttctg atgaactcgc
    31741 aatggcgtcc cgatcgctgc gcggtcattg ccggggcgct gcgttaccca cgttatcgct
    31801 ggtacgaccg ttttatgatc aagctgatta tgaagatgtc aggcggtgaa acggatacgc
    31861 gcaaagaagt tgtctatacc gattgggagc aggtggcgaa tttcgcccga gaaatcgccc
    31921 atttaaccga caaaccgacg ctgaaataag cataaagaat aaaaaatgcg cggtcagaaa
    31981 attattttaa atttcccctt gtcaggccgg aataactccc tataatgcgc caccactgac
    32041 acggaacaac ggcaaacacg ccgccgggtc agcgggattc tcct
//
LOCUS       DBFIFM010000035        31731 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.035, whole genome shotgun sequence.
ACCESSION   DBFIFM010000035 DBFIFM010000000
VERSION     DBFIFM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.035"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            116..1498
                     /locus_tag="N7C74_001413"
     CDS             116..1498
                     /locus_tag="N7C74_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HGU2431311.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDNFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLCGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            1508..3826
                     /gene="yicI"
                     /locus_tag="N7C74_001414"
     CDS             1508..3826
                     /gene="yicI"
                     /locus_tag="N7C74_001414"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HGU2431312.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAGRNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTALAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFSEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDNTLLALGNNEQRPDYAW
                     HEGTAFQLFNLQDGHEAICEVPAADGSVLFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(3879..5588)
                     /locus_tag="N7C74_001415"
     CDS             complement(3879..5588)
                     /locus_tag="N7C74_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HGU2431313.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSSEVTSTE
                     PVQ"
     gene            complement(5709..7100)
                     /gene="xanP"
                     /locus_tag="N7C74_001416"
     CDS             complement(5709..7100)
                     /gene="xanP"
                     /locus_tag="N7C74_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HGU2431314.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            7380..8585
                     /gene="gltS"
                     /locus_tag="N7C74_001417"
     CDS             7380..8585
                     /gene="gltS"
                     /locus_tag="N7C74_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HGU2431315.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            8588..9454
                     /locus_tag="N7C74_001418"
     CDS             8588..9454
                     /locus_tag="N7C74_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2431316.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(9439..11520)
                     /gene="recG"
                     /locus_tag="N7C74_001419"
     CDS             complement(9439..11520)
                     /gene="recG"
                     /locus_tag="N7C74_001419"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HGU2431317.1"
                     /translation="MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(11526..12215)
                     /gene="trmH"
                     /locus_tag="N7C74_001420"
     CDS             complement(11526..12215)
                     /gene="trmH"
                     /locus_tag="N7C74_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HGU2431318.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(12222..14330)
                     /gene="spoT"
                     /locus_tag="N7C74_001421"
     CDS             complement(12222..14330)
                     /gene="spoT"
                     /locus_tag="N7C74_001421"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HGU2431319.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(14349..14624)
                     /gene="rpoZ"
                     /locus_tag="N7C74_001422"
     CDS             complement(14349..14624)
                     /gene="rpoZ"
                     /locus_tag="N7C74_001422"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HGU2431320.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(14679..15302)
                     /gene="gmk"
                     /locus_tag="N7C74_001423"
     CDS             complement(14679..15302)
                     /gene="gmk"
                     /locus_tag="N7C74_001423"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HGU2431321.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRDQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            15560..17242
                     /gene="ligB"
                     /locus_tag="N7C74_001424"
     CDS             15560..17242
                     /gene="ligB"
                     /locus_tag="N7C74_001424"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HGU2431322.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEIFRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAKVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(17239..17856)
                     /locus_tag="N7C74_001425"
     CDS             complement(17239..17856)
                     /locus_tag="N7C74_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HGU2431323.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(18148..18972)
                     /gene="dinD"
                     /locus_tag="N7C74_001426"
     CDS             complement(18148..18972)
                     /gene="dinD"
                     /locus_tag="N7C74_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HGU2431324.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAAVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(19193..20056)
                     /locus_tag="N7C74_001427"
     CDS             complement(19193..20056)
                     /locus_tag="N7C74_001427"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HGU2431325.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            20183..20899
                     /gene="rph"
                     /locus_tag="N7C74_001428"
     CDS             20183..20899
                     /gene="rph"
                     /locus_tag="N7C74_001428"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HGU2431326.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            20965..21606
                     /gene="pyrE"
                     /locus_tag="N7C74_001429"
     CDS             20965..21606
                     /gene="pyrE"
                     /locus_tag="N7C74_001429"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HGU2431327.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(21643..22239)
                     /gene="slmA"
                     /locus_tag="N7C74_001430"
     CDS             complement(21643..22239)
                     /gene="slmA"
                     /locus_tag="N7C74_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HGU2431328.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(22346..22804)
                     /gene="dut"
                     /locus_tag="N7C74_001431"
     CDS             complement(22346..22804)
                     /gene="dut"
                     /locus_tag="N7C74_001431"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HGU2431329.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(22782..24002)
                     /gene="coaBC"
                     /locus_tag="N7C74_001432"
     CDS             complement(22782..24002)
                     /gene="coaBC"
                     /locus_tag="N7C74_001432"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HGU2431330.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            24174..24842
                     /gene="radC"
                     /locus_tag="N7C74_001433"
     CDS             24174..24842
                     /gene="radC"
                     /locus_tag="N7C74_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGU2431331.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            25059..25295
                     /gene="rpmB"
                     /locus_tag="N7C74_001434"
     CDS             25059..25295
                     /gene="rpmB"
                     /locus_tag="N7C74_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HGU2431332.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            25316..25483
                     /gene="rpmG"
                     /locus_tag="N7C74_001435"
     CDS             25316..25483
                     /gene="rpmG"
                     /locus_tag="N7C74_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGU2431333.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            25581..26390
                     /gene="mutM"
                     /locus_tag="N7C74_001436"
     CDS             25581..26390
                     /gene="mutM"
                     /locus_tag="N7C74_001436"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HGU2431334.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(26429..26908)
                     /gene="coaD"
                     /locus_tag="N7C74_001437"
     CDS             complement(26429..26908)
                     /gene="coaD"
                     /locus_tag="N7C74_001437"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HGU2431335.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(26916..28193)
                     /gene="waaA"
                     /locus_tag="N7C74_001438"
     CDS             complement(26916..28193)
                     /gene="waaA"
                     /locus_tag="N7C74_001438"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HGU2431336.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            28606..29664
                     /gene="rfaQ"
                     /locus_tag="N7C74_001439"
     CDS             28606..29664
                     /gene="rfaQ"
                     /locus_tag="N7C74_001439"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HGU2431337.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            29661..30785
                     /locus_tag="N7C74_001440"
     CDS             29661..30785
                     /locus_tag="N7C74_001440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGU2431338.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            30778..31575
                     /gene="rfaP"
                     /locus_tag="N7C74_001441"
     CDS             30778..31575
                     /gene="rfaP"
                     /locus_tag="N7C74_001441"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HGU2431339.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVHDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         7484 a         7871 c         8517 g         7859 t
ORIGIN      
        1 acataaaaca agaatcataa attaataacc agatatcgga atattcgctc tcacagggat
       61 ggtttacaaa atgcgttatc ggtctcagca cccctatagc atcaaggaaa agcagatgaa
      121 gagtgaagtg ttgtccgtta aagagaaaat tggttatggc atgggagacg ccgccagcca
      181 cattattttc gataacgtaa tgttatatat gatgttcttt tataccgata attttggcat
      241 acctgccgga tttgtcggaa ccatgttttt ggtcgctcgt gcactggatg cgatttccga
      301 tccttgcatg gggttgttgg ccgatcgaac gcgctctcgc tggggtaaat ttcgtccgtg
      361 ggtactgtgt ggcgcactgc catttgggat cgtctgtgta ctggcctata gcacgccaga
      421 tctcagtatg aacggcaaaa tgatctatgc agcaattact tacaccctac ttaccttact
      481 ttataccgtc gtcaatatcc cttactgcgc attgggtggt gtaatcacca atgacccgac
      541 tcagcgtatc tcgctgcaat cctggcgttt tgtgctggcg accgcgggag gcatgctctc
      601 tactgttctg atgatgccac tggttaattt aattggcggt gataataaac cactcggttt
      661 ccagggcggt atcgcggtcc tttccgtggt ggcattcatg atgctggcat tctgtttctt
      721 caccactaaa gaacgcgttg aagcaccacc tacaacgacg tctatgcggg aagatttacg
      781 tgatatctgg caaaacgacc agtggcggat tgtcggttta ctaaccattt tcaatatcct
      841 ggcggtgtgc gtccgcggtg gagcgatgat gtattacgtc acatggattt tgggtacgcc
      901 ggaagtgttt gtcgcttttc tcaccactta ttgcgtgggt aacctgattg gttccgcact
      961 ggcaaaaccg ctgaccgact ggaaatgtaa agtcactatc ttctggtgga cgaacgccct
     1021 gctggcagtg attagcctcg cgatgttctt tgttcctatg caggccagca tcactatgtt
     1081 tgtcttcatc ttcgtgattg gtgtattgca tcaactggtg acacctatcc agtgggtaat
     1141 gatgtccgat accgtcgact acggcgagtg gtgcaatggt aaacgcctga ccgggatcag
     1201 ttttgctggc acgctgtttg tgctcaaact ggggttggcc ttcggcggcg ctcttatcgg
     1261 ctggatgctg gcttatggcg gatatgatgc ggcagaaaaa gcgcagaaca gcgccacgat
     1321 tagcatcatt attgcgctat tcacgattgt tccggcgatc tgttatttgc tgagcgcgat
     1381 tatcgctaaa cgctactact cactcacgac gcacaatctg aaaaccgtta tggaacagct
     1441 ggcccagggc aaacgccgtt gccagcaaca attcacctct caagaagtgc agaactaagg
     1501 aacggcaatg aaaattagcg acggaaactg gttgattcaa cctggcctca atttgattca
     1561 cccgcttcag gtgttcgagg ttgaacagca gggtaatgaa atggtggtct atgctgcccc
     1621 ccgtgatgtg cgtgaacgaa cctggcagct tgatacgcct ttatttacgc tgcgcttttt
     1681 ctccccacag gaaggtattg tcggtgtacg gattgagcat tttcaggggg cgctgaataa
     1741 cggtcctcat tatccgctca atattttgca ggacgtgaag gtcacaatcg aaaacacaga
     1801 acgttacgct gagtttaaaa gtggcaactt aagcgcgcgt gtcagcaaag gtgagttctg
     1861 gtcactggat tttctgcgca acggcgaacg tattaccggt agtcaggtga aaaataatgg
     1921 ctacgtgcag gacacgaata atcaacgaaa ttatatgttt gagcggctgg atcttggcgt
     1981 tggcgaaaca gtttacggtc tgggagagcg ctttactgcc ctggtgcgca atggccagac
     2041 ggtagagacc tggaaccggg acggcggcac aagtactgaa caggcgtata aaaatatccc
     2101 gttctatatg actaaccgtg gctatggggt actggtcaat catcctcaat gcgtctcttt
     2161 tgaagtggga tcggagaaag tctccaaagt gcagttcagc gttgagagtg aatatctcga
     2221 atactttgtt atcgacggcc cgacgccgaa agcggtactt gatcgttata cccgttttac
     2281 tggtcgtccg gcgctgccgc ccgcgtggtc cttcggcctg tggctaacca cttcatttac
     2341 caccaactac gacgaagcga cggtaaacag ctttatcgat ggtatggcgg gacgcaatct
     2401 gccactgcat gtttttcact ttgactgttt ctggatgaaa gccttccagt ggtgcgattt
     2461 tgagtgggac ccgctgactt tcccggaccc ggaagggatg atccgccgcc tgaaagcgaa
     2521 agggctaaaa atctgcgtct ggattaaccc ctatatcggg caaaaatccc ctgtctttaa
     2581 agagttacaa gagaaaggct atttactcaa acgcccggac ggttcgttat ggcagtggga
     2641 taaatggcag ccaggtctgg cgatttatga ctttaccaat ccggatgcct gcaaatggta
     2701 cgccgacaaa ctgaaaggtc tggtcgcgat gggcgttgat tgctttaaga ccgactttgg
     2761 cgaacgtatt ccaatcgatg ttcagtggtt tgacggttcc gatccgcaga aaatgcataa
     2821 ccattatgcg tacatctaca acgaactggt gtggaacgtg ctcaaggaca ccgttggcga
     2881 ggaagaagcc gtcttgtttg ctcgctcggc ctccgttggt gcgcagaaat tcccggtaca
     2941 ctggggtggt gactgttacg ctaactacga atcaatggcg gaaagcctgc gcggtggttt
     3001 gtctattggc ctttcaggtt ttggtttctg gagccacgat atcggcggct ttgagaatac
     3061 cgctctggcg cacgtttaca aacgctggtg cgcgtttggt ttgctctcca gccatagccg
     3121 tttacatggc agcaaatctt atcgtgtgcc gtgggcctac gatgatgagt cctgtgatgt
     3181 ggtgcgcttc ttcacgcaac tgaaatgccg catgatgccg tatctgtatc gtgaagctgc
     3241 tcgtgcgaac gcgcggggta cgccgatgat gcgggccatg atgatggagt tcccggacga
     3301 tccggcttgt gattaccttg accgtcaata catgttaggc gacaacgtga tggttgctcc
     3361 ggtgttcagt gaagcgggcg atgtgcagtt ctacttgccg gaaggtcgct ggacacatct
     3421 gtggcacaac gatgaactcg atggtagtcg ctggcataaa cagcagcaca gcttcctgag
     3481 tctgcccgtt tatgtgcgtg ataacaccct actggcgctg ggcaacaacg agcaacgtcc
     3541 cgattacgcg tggcacgaag gcacggcatt ccagctcttt aatctacaag acgggcatga
     3601 agccatctgt gaagtgcccg ctgctgacgg ttccgttctt ttcaccctga aagcggcgcg
     3661 tactggcaac acgattactg tgactggtgc gggcgaggcg aagaactgga cactgtgctt
     3721 gcgcaatatt gtgaaagtaa atggtctgca aggtggttcg caggctgaaa gtgagcaggg
     3781 gctggtggtg acgcctcaag ggaatgcgct gacaattacg ttgtaatcta ttgatggaat
     3841 gccgggtacg tctgagtcgt acccggcgag attgattttt actgcaccgg ctctgtcgat
     3901 gtaacttcgc tactggaaac gacgcccgca ttcatggttt gagtcacttg ctgctttgcg
     3961 gcgttcacgg catggagttg cccgctaacc gtaggtttca gcgggacatt ggcctgaata
     4021 tcgccactgg cggtaagctg aatattgccg tcgccagtca acggtaattc aggccatccc
     4081 cattgttgca aaatattcac cggcacaccg cgtccattca ggctgatatg ggtctgacgt
     4141 tgtggcgttt gtgaaacact ggcagtggct tccagaatgc ctttttcagt aaatgcactc
     4201 agttcgctga tattcaccgt gctgctgttg gcggtcagcg ccagcgacgg gcgacgaaca
     4261 tcaacacgat tgaatgtcgc ggcggcggca ttcagattcg ccgagccact ccagacgccc
     4321 catttatgat cggtaaccag cgtcaggttg gcaccgtaac catcgagcgt ggtgagctgc
     4381 cacgggaagt cagggtcgat atcaatgatc agattgcggc tggcgctaaa tctcttcagt
     4441 tgcaggctgt ttaaccaacc gggtgtcgtt tccatccaca actgttgcca gtttttcggc
     4501 aaggtatatt ccagcccggc aattgccgca tcatcaagga tcaacgtttt cccgtcacgc
     4561 agccagttcc ctgacgttct gaccataccc ccttcccagc ggctggtgaa ctggcgcagc
     4621 gctacgccct gcggggaaaa ttccgcgttt ataatcgggt caaataaatg cagcgaacca
     4681 taaatgaact cgctagcgtt catcgacagt ttgccttctt gtgtctgcca gtcatcttta
     4741 ctgaaggtca tgttgcgcaa gctgagatcg aggtcggtca ccgcccagtc cggaccttgc
     4801 aaacgagcat cgatcacttc caggcgacca atctgcaacg acgggacaga gcgtaatggc
     4861 gcaaagaagt cggttagcga tttttcgctt tgtagacgga tatccgccat gcgcagattt
     4921 tccacttgcc agctgccgtc ggcgttacgc tgcgcgtttc cggttaatgt cccgcgggcg
     4981 atgtcggcac ccaggttagt cagcgtaacg cgatcgttat caatactgcc ttcaatcagt
     5041 acattggtgg caggaacatc attgagcgaa agcgatccgg cactaaactg aatctgcgcc
     5101 ttcgtaccca gcactttacc ggcttctggt gaccacggaa ccacgccgcc atttacccgc
     5161 tgcgcgctca gtttccattc gctattcggg ctattaaacg ccatatcacg cagttgcaga
     5221 cgatcggctt tgaacggtag cggcgcggtc tggtcggtga gattcagcgt cccgttttcc
     5281 agcaggatgg tatcgacatg gcgtggttcg gtcagttgcc gactgcttag cgcaatgtcg
     5341 acactttttg ccaccagggt cgcgggctga ccatcacgac caaacgtgac gttctccagc
     5401 acgatatgag atggcgcgga aaaacggtga tccatcgccc cgaaggccag atgatagtcg
     5461 ctattctcgg aaacccatgc gctgatatgt tctgctcccc agcgggtttg cagaagaaaa
     5521 tagaggccag cgatcaccac taacagagcg atgagaatgt agagaagcag cttcccaata
     5581 aatttcatgg tcttccatcc cgtgaaatgc acataaggga gttatgcacg atttacgcgc
     5641 aatcctcaag gcgggaatgg tgaaagaggt gagactgcgg cggtaattat cttaccgccg
     5701 ccagtgaatt actgtttttc tggtgggaaa atcagattca gcacgatggc agtaataccg
     5761 cccgcggcga tcccggagga gagcaggttt ttcagccatt caggggcaaa ctgcaaaatc
     5821 agcggctgct gagacacgcc cagaccaacc gccagcgaca gcgcgataat cagaatcgcc
     5881 cgacggttca gcggctcacg agaaacgata cgcacaccgg aggcggcgat ggtgccaaac
     5941 attacaagcg ttgcgccgcc cagaaccggt tctggaatgt gttgtacaaa accgctcact
     6001 gccgggaaca gacccagcac gatcaacatc agcgcgacga caaaaccgac atagcggctg
     6061 gcaacaccag tcaactggat cactccgttg ttttgcccga agcaggagtt cgggaaggtg
     6121 ttaaacaccg ccgaaacaaa cgagttcagg ccgtttgcca gcacgccgcc tttcaggcgt
     6181 ttcatgtaca gcggaccgga cactggctgt tcggaaacgt cagaggtcgc cgtgatatcg
     6241 ccaatggttt ccagcgaagt gatcataaag accagcatca gcggcagcag cagactccat
     6301 tcaatgccaa gaccgtaata gagcggcgtt ggcaccataa tcagttcttg cgtcatcggt
     6361 tcgttgcttt ctggcaacat gcccataaac cacgccagcg catatccggc cgccatcgca
     6421 atcaccagtg aggccacgcg taagtaaggg ttacgttgac ggttaagcag gataattaag
     6481 gctaagacca cgcctgccag cagcagattt ttcggtgcgc cgaaggtgtt atcgctcatg
     6541 gctgcgtaac cgccgccaat ggacgttaac ccaacctgaa ttagcgacag gccgataatc
     6601 atcaccacaa cgccagaaac cagcggcgta ataatgcggc gcgccagatg cagaacgcgg
     6661 gagatcacca tctcggtgca acttgccagc atcaacgtgc cgaacaaagc cgccatcatg
     6721 gtaggaacat cagcaccacc ggttttcagc gcggtaccgc ccataatcag cggggcaaca
     6781 aagttgaagc tggtgccctg aatagacaac agcccggagc caaccggacc ccaggcctta
     6841 atttgaataa tcgatgccac accggaggca aacagcgaca tactaataat gtgttgcgtg
     6901 tcttgtgccg gtaaacccag cgcctggcag attaatagcg ctggcgtgat caccgcaacg
     6961 aacatcgcca gcagatgctg acaggcggca aacagggttt gaggaagcgg cggacgatct
     7021 tcaagacggt aaatcagttc gctgttttga gtctgcgcaa ccggttgcgc attttctgac
     7081 tcgagggtgg aaacagacat cgaaaacaat cccgtggtag caaagcgggc attttagctg
     7141 accgaaagag aaaagcaaac gtttgccaca tcctcatcta aatatttaaa tgagataaat
     7201 gcactttttt atataacttt tggtattttt ctgcttaaaa tccatgccat ttgttcatta
     7261 acagctgaga atttgaccca ggaaagcata actcgatacc cgtcatagtt catgaagttg
     7321 ccactgcgcg atgaagtatg acgagtatga aagagtgatg cggatacaaa ggagtaacta
     7381 tgtttcatct cgatacttta gcaacgcttg ttgccgcaac gctgacgttg ttgctcgggc
     7441 gtaagttggt ccattccgtc tcctttttga agaaatacac cataccggaa cctgttgcgg
     7501 gtggtttgtt ggtggcgctg gcgctactag tactgaaaaa aagcatgggc tgggaagtca
     7561 actttgatat gtccctgcgc gatccgttaa tgctggcttt cttcgccacc attggcctga
     7621 acgccaacat tgccagtttg cgtgccggtg ggcgtgtggt tggcatcttc ttgattgtgg
     7681 ttgttggtct gttggtgatg caaaatgcca ttggcattgg tatggctagc ctgttagggc
     7741 ttgatccgct gatggggctg ttggccggtt ctattacgct ttccggcggt cacggtacgg
     7801 gcgctgcgtg gagtaaattg ttcattgaac gttatggctt caccaatgcg acggaagtgg
     7861 cgatggcctg tgcaacgttc ggtctggtgc tgggcggctt gattggcggt ccggtggcgc
     7921 gctatctggt gaaacactcc accacgccga acggtattcc ggatgaccag gaagtcccga
     7981 cggcgtttga aaagccggat gtgggccgca tgatcacctc gctggtgctg attgaaacta
     8041 tcgcgctgat tgctatctgc ctgacggtgg ggaaaattgt tgcgcaactt ttggctggca
     8101 ctgcttttga actgccgacc ttcgtctgtg tactgtttgt tggcgtgatt ctgagcaacg
     8161 gtctgtcaat gatgggcttt taccgcgtct ttgagcgtgc ggtatccgtg ctgggtaacg
     8221 taagcctgtc gttgttcctg gcgatggcgc tgatggggct gaaactgtgg gagctggctt
     8281 cgctggcgct gccgatgctg gcgattctgg tggtacagac catcttcatg gcgttgtatg
     8341 ccatcttcgt tacctggcgc atgatgggca aaaactacga tgcggcagtg ctggctgcgg
     8401 gtcactgtgg ttttggcctc ggtgcaacgc caacggcaat cgccaacatg caggcgatca
     8461 ctgaacgctt tggcccgtcg cacatggcgt ttttggtggt gccgatggtc ggtgcgttct
     8521 ttatcgatat cgtcaatgcg ctggtgatta agctgtattt aatgttgccg atttttgccg
     8581 gttaaaaatg aaaaagcgtc ggaaggaacc ggaaacgcta cgtgaacact gtcgccatat
     8641 ttttggtgat gagccgccag tgctctgtgt atgggaaacg gaatttgatt acgcagatgc
     8701 tgaactcaag gcgctggcgg ctaaagagtg gcagcagatc agcgaatggg atttgagtgc
     8761 ttactatgta ctcaatctcg tttataacga accgatgcaa atagagttgt ttcgctatct
     8821 gtttccgctc tgcctggcgc aatggcatga aaccgtttta gctggcggtt atggtgatca
     8881 cttcgaagaa tccttaatga aggcattgtg tcggccctat ctctggcagg agatgatgaa
     8941 tgcttcccaa cgccagcagg tacggcaatt tttactggac accgcgttgc agcgtatgga
     9001 taacgagcgt ggtttcaata acgttctttg ctggctggcg gtgttcaata ctctgggtgg
     9061 ggccgcgccg ttaattcgtt ctctctggtc gcgctggtgg gcgctggata cgccaggaaa
     9121 agcggtgtgt gctattcagt atgccgcgca tctaatttat cccatagaag ccaatccgtt
     9181 atggtcgcaa gaatggatag gctgggggca tccattaggc cataaagatg gctggtcctc
     9241 tgataatcgc gcttttcttc gccaaatgtt aacacctgaa atgattgtgg ctggcgtaca
     9301 ggcagcggct gaaattctgc gtggtgaacc ggaaggcgca atggcggcgc gaattgcaca
     9361 ggatgcgtat gaggcaatgg atatcctcac tatccagatt gaagatttac tgcgtgactt
     9421 gtcttgtgat gagtctggtc atgcgttgga gtaacgttcc gtctccggca tccagcgttc
     9481 tatcagggct tttgcttgtt gtgggtaacg ttcgtgaata tgtcgcgcca ggcgctgaac
     9541 ttccgggatc atcgcctgat cgcgcagtaa atccgccact ttaaactcag cattacccgt
     9601 ctgacgcgta cctaacagtt cacccggtcc gcgaatctct aagtctttct gtgcaatcac
     9661 aaaaccgtcg ttgctgtcgc gcagcacttg cagccgaatt tgcgcggttt tggagagcgg
     9721 cgttttgtag agcagcacgc agtgagaagc caccgcacca cgacctacac gcccgcgaag
     9781 ctggtgtaac tgcgccagac ccagacgctc agggttttcg ataatcatca ggctggcgtt
     9841 aggcacatca acaccgactt caataacggt agtggcaact agcagatgta gctcaccttg
     9901 tttaaacgac gccatcaccg cctgtttctc ggctggtttc atccgcccgt gtaccaggcc
     9961 aacattcaat tctggtagcg ccagtttcaa ctcttcccag gtagcttccg ccgcctgcgc
    10021 ttccagtaac tctgactctt caatcaacgt acaaacccag tacgcctgac gaccttcagt
    10081 tatgcaggcg tggcgcacgc ggtcaatgat gtcggtacgg cgggtatcag gaatagcgac
    10141 cgtagtcact ggcgtgcggc ctggcggcag ctcatctatc accgaggtat cgagatcggc
    10201 atacgccgtc attgccagcg tgcgggggat cggcgtggcg gtcatgatca actgatgcgg
    10261 atggaagcct tgctgctggc ctttctccca taacgccaga cgctgatgca cgccaaagcg
    10321 atgttgttcg tcgataatca ccagcgccag accgttaaac tgcacctgtt cctggaagat
    10381 ggcgtgcgta ccaacaatca tctgcacctg accgctggcg atggcttcct gctgagacag
    10441 tcgcgctttg cctttctgct taccggcgag ccagcccact tcgataccga gcggttcaaa
    10501 ccagttgcgg aagttattgg cgtgctgttc ggcgagtaat tcggttggtg ccatcaatgc
    10561 cacctgtttg ccgtgggcaa tcgcacgcag cgcggcgagg gcggcgacca gcgttttacc
    10621 ggaacctaca tcgccctgta ccaggcgcat catcggcaca tccagcgcca tatcgcgctc
    10681 gatctccgcc actacgcgtg cctgtgcgcc cgttggcttg aacggtaagg ctgcgaggag
    10741 tttatttttc agcgcatcat tggcgctcag cggctgggca tgaaaacgct gtgctccggc
    10801 acgtaaggct aacatgctga ggttgtgcgc cagcagttct tccagaatca gacgacgttg
    10861 cgctggatgc tgcccggttt ccagatcgct aagctgtagc gtcggtggcg ggcggtgcaa
    10921 agtgcgcaac gcttccggta gcgtcatcat tccttgtgac agttccggcg gcaggagttc
    10981 ttcaatggcg caggtgtcga gcagatccag cgcctggtcg gttaatttac gcagcgtggc
    11041 ctgctttacg ccttccgttg ttggataaac cggcgtgagc gtttcctgta attctggcgt
    11101 gctgagatcg ccctgcacgc ggtattccgg gtggatcatc tccgcaccat atttaccgcg
    11161 ctttgcttcg ccataagcca gtacacggcg gcccgtcgcc aggctatttt tcattgccgc
    11221 gctgaaattg aaaaagcgca tggtgaggat gccggaaccg tcgctgatct ggcaggtcat
    11281 catccgccga ccgccgaagg agatattgca gttcagcact tcgccttcca ccgtggcata
    11341 aacgcccggc agtagttctc cgatggggta gagatgggtg cgatcttcgt agcgcagggg
    11401 aaggtgtaag agtagatcct gcacggtatg caggttgatt ttcgccagtt tgttactaag
    11461 tgctgcgcca acgcccgtta gggaactgag tgggacagca tctaacaggc gacctgtcat
    11521 ggcacttacc ctgcagcctg catagtagcc caccagtcgg catcagcttc gatctcgcct
    11581 tgctgattga cgtggggata aggcaggcct ttgcgttttg cgactttcgc cagcaccgga
    11641 tagccgcctt caaacaacag gcgttgttgc tctgcttccg gcaacatgct gttttcacgc
    11701 aggtacatgc ctgcattttg ccgctgacgc tgggcttcgt aaagaatgag ggctgaggca
    11761 acggaaacat tcagcgactg caccatgccg atcatcggaa tgatgatgtc ctgatccgcc
    11821 agggccaatg cttcctgcgt gatgcccgtt ttctcctgtc ccatcaaaat gcaggtcggg
    11881 cgagtgtaat caatttcgcg gaaatcgaca gcgttatcag aaagatgggt tgccagaatc
    11941 tgcatgccct ggcctttgag atgagcgacg gcatcgccaa tggtgcggtg tgttttcacc
    12001 tgtacccagc tgttactacc cgccgctgcc gaagccatgg tgcgcatgcg gctaccaggc
    12061 cagacggcgt gaacttcatg tacgccaacg gcatctgcgg tacgaataat cgcagaaacg
    12121 ttatgaggtt tgtggacctg ctccatgcag acggtcagat caggctgccg cctggcgagc
    12181 atttcgcaga tgcgtgcata acgtgttggg ttcataaaac attaatttcg gtttcgggtg
    12241 actttaatca cgtctggcat cacgcggatt ttgcgcatga tattcgccag atgcacacgg
    12301 tcacgagcgg tcagacgaat aaaggcgctg tagacgcgac catctttctc ttccgtattc
    12361 aaactttgaa tattcgaggt cgtggtgtta attgccgccg tcaggtttgc cagcgcaccc
    12421 tgatgattga acatctccac cttgatttcg gtgatgaact cctgcgccgt ctctttatcc
    12481 cattccacag ccataaactt ctctggctct ttctggtagc cacggatgtt acggcaggat
    12541 tcatggtgga tcaccagacc tttaccgggg ctgacgtggg cgataatcgg gtcgccagga
    12601 atggggcggc agcatttcgc aaaggtgatc agcacgccat cagcgccttt gatcggcaga
    12661 tgcccgtggc tttgagtcgc cggtggaatg gaggcgtccc catgttgtag atttttcgcg
    12721 accaccacgc tcattgcgtt accaaggccg atttctgcca gcagatcgtc aagcgttgcc
    12781 agcttcatgc gatccagctc gcgctgaata ttttcctgcg ggatttcatt gagcttacgg
    12841 ctaccaccca aagcatggtt gagcagacga cggcccaggc ttacagaatc atcacgcttg
    12901 aggtttttca gcaactgacg aattttggcg cgcgctttcg agctaacgac aaagttcagc
    12961 caagcggcat tcgggcgagc gcccggagcg gtaatgattt caacggtctg accgctagta
    13021 agcggctgcg acagcgggta aggctggcgg tcgacgcgtg cgcccacgca ggcatggccg
    13081 atatcggtat gcactgcata agcgaagtcg acgggcgttg caccggcagg cagctcgaca
    13141 atgcgccctt ccggtgtgaa aacgtaaatc tcatccggga agagatcgga tttaacgctc
    13201 tcgataaatt caaacgaact accggcgctc tgttgcagct ccagcaggct ttgcatccag
    13261 cgctgggcgc ggatttgtgc ggtagtactg gtttcgccgt gctctttata agcccagtgc
    13321 gcggcaacac ccatctccgc catctgatcc atatcttcgg tacggatctg gacctcaacc
    13381 ggcacgccgt gcgggccaat catcgaggtg tgcaacgact gatagccgtt cgcttttgga
    13441 atggcgatat agtctttcac gcggcccgga cgcggcttgt acaggctgtg catctggccc
    13501 agcacgcgat aacaggtgtc agaatcattg acgatcacgc ggaaagcgta gatgtccatg
    13561 attgagtgaa aacgctgctc tttgagcacc attttacagt aaatcgaata aagatgcttt
    13621 tcgcgaccac tgacgcggca cggtattccc gcttcctgca aacgcccttc gatttcagaa
    13681 agaatcttct ggatcatctc tttacggttg ccgcgcgcgg ctttcaccac ttctttgatt
    13741 acgcgataac ggttgggata cagcgcctca aaacccagct cttcgagttc ggttttaatg
    13801 tggtggatac ctaaacggtg cgccagcggg ctgtaaattt cgagagtttc acgggcgatg
    13861 cggcgacgtt tgtccgggcg aagtgagccc agcgtgcgca tgttgtgggt acggtcggca
    13921 agtttgatga ggatgacgcg gatatcctgc accatcgcca taatcatctt gcgaaagttt
    13981 tcggcctgcg cctctttctt atcgcggaac ttgagtttat caagtttcga caccccctct
    14041 accagctcgg cgacgctttt accaaaaagc tgttccatat cctggtaggt ggcgggagta
    14101 tcttcaatca cgtcatgcag cagcgccgcc atcagcgttt catagtcgag tttcatctcg
    14161 gccagaatgc aggcaaccgc taccgggtgc gtgatatagg gttcaccgct tgaacgtgtt
    14221 tgcccctcgt gagcatcacg tgcaacgaga tacgcctgcc gcagacgctt gatttggtct
    14281 tccggcaggt aggtttgaat cagttgattc aggctttcaa acagatacaa gggcgacccg
    14341 ctttgtgatt aacgacgacc ttcagcaata gcggtaacgg cttgtaattc agcggcttcc
    14401 tgctcttgct gttcctggcg ttcgcgaacg tcgaggatct ggttgttgat cagaccttct
    14461 tcgatttcgc gcagcgcgat tacagtggtt ttatcgtttt cttccggtac cagcggatcc
    14521 tttccgccta cctgcatctg acgagcgcga cgcgcggcga ccagtaccag gtcaaaacgg
    14581 ttaccaattt tctctacagc gtcctgaaca gttacgcgtg ccatacttaa aaagctccac
    14641 aggtgaagaa atgactgggc atgatactga aatcaggttc agtctgccaa caatttgctg
    14701 attaaagcgt catgacgctg cttttggcgg ctcatgcgca gacgttcggc gcgaataatg
    14761 gtcttcaaat cggtcaacgc ggtatcgaag tcatcattca caatcaggta atcatattcg
    14821 gcgtaatggc tcatttctgc aacagcttgc gccatacgct ttgcaatgac ctcttcgctg
    14881 tcctgatcgc gaccgcgtag acggcggtcc agttcaattt tagacggcgg taaaataaag
    14941 atactccgcg cgtgcggcat cttctggcga atttgctgcg cgccctgcca gtcgatatcg
    15001 agaaaaacat cgacaccggt cgccagtact tgctcaatgg cctcacgcga agtgccatag
    15061 taattaccaa aaacttctgc gtgttcgagg aacgcatctc tgctaatcat ttctttaaat
    15121 tcatcatgat taacaaagaa ataatgttca ccgtggactt caccaggacg cggttggcgt
    15181 gtggtgtgtg aaacagaaac ctgggtgtca tacaacggtt gggtttttaa taaagcctga
    15241 atcaggctgg atttacccgc gccactgggg gcagaaacaa tataaagcgt gccttgagcc
    15301 atgagtatct ttcgtatgtg attagcgaaa taaagcctac atacgagctt attatacacg
    15361 gcgcggccat gtgacgtagc ccttgctcac actttttgcg caaagttgtt gcgttttgcg
    15421 tagctgtttc cgcatttgcc tgtgatttac tgcaataaag acgattctca ggaagcgtcc
    15481 tcgcagactc cgtttttgcc actcggcttt gccttcacca cgttgtatat ctgccagcct
    15541 gactacagcg gaggtgaaga tgaaagtatg gatggcgata ttaataagta tcttgtgctg
    15601 gcaatcatct gtgtgggcgg tctgtccggc ctggtcgcca gccagagcac aggaagaaat
    15661 tttccgcctg caacagcaaa taaaacagtg ggacgatgac tactggaagg aaggaaaaag
    15721 tgaggtggaa gacggtgttt acgatcagtt aagcgcccgt cttacgcagt ggcaacgctg
    15781 ctttgtgagc gagccccgcg acgtgatgat gccgccttta aatggtgcgg ttatgcatcc
    15841 ggttgcccat acgggtgtac gcaaaatggc ggataaaaac gcattaagtc tgtggatgcg
    15901 agaacgtagc gatctttggg tgcagccaaa agttgatggc gttgcggtaa ccctggttta
    15961 tcgggacggg aaactgaaca aagcaatcag tcgcggtaat ggcctgaaag gcgaggactg
    16021 gacgcagaaa gttagcttaa tttccgctgt gccgcaaacc gttagcgggc ctttagccaa
    16081 cagtacgctt cagggggaaa tatttctcca gcgcgagggg catatccaac aacaaatggg
    16141 gggaataaat gcccgcgcaa aagttgctgg cctgatgatg cgccaggacg atagcgacac
    16201 gctgaattct ctgggcgttt ttgtctgggc atggccggat ggaccgcagt taatgaccga
    16261 tcgtttaaag gagctggcta ccgcaggttt tactctaacg cagaggtata cccgtgcggt
    16321 taaaaatgct gatgaggttg cgcgcgtacg caatgagtgg tggaaagcga aattaccctt
    16381 cgtcaccgat ggcgtggttg tgcgaggggc gaaagaaccc gaatcccgcc attggctacc
    16441 aggccaggca gaatggctgg tggcctggaa atatcaacct gtagctcagg ttgccaaagt
    16501 gaaggcgatt cagtttgcgg tgggtaagag cggtaaaata tcggtggttg cgtcactcgc
    16561 acctgtcatg ctggatgata aaaaagtcca gcgggtgaat attggttccg tcaggcgctg
    16621 gcaggagtgg gatattgcgc ctggtgatca gatcctcgtc agccttgccg gtcagggtat
    16681 tcctcgcatt gatgatgtgg tctggcgcgg tgcagaacgt acaaaaccga caccaccaga
    16741 aaaccgcttt aactcgttga cctgctactt tgcttctgat gtttgtcagg aacagttcat
    16801 ttcacgctta gtctggctgg ggtcaaaaca ggttctgggg ctggatggca ttggtgaggc
    16861 cggctggcgc gcgctgcatc agactcatcg ctttgagcat atcttttcct ggcttttatt
    16921 aacgccagag caattacaga acacgccggg gatcgcgaaa agtaaaagtg cgcagctatg
    16981 gcatcggttt aatctggctc gtaagcagcc ttttactcgc tgggtgatgg caatgggaat
    17041 accgctaacc cgggcggcgc ttaatgccag tgatgaacgg tcctggtcgc aacttttatt
    17101 tagcacggag cagttctggc agcagctgcc ggggactgga tcggggcgcg ccagacaggt
    17161 tattgaatgg aaggaaaatg cgcaaatcaa gaagctgggc agttggctgg ctgcccagca
    17221 gatcacaggt tttgaacctt agtgtccttc gtggctgtag taaaagaccg gtaatcccag
    17281 cttaaggcgt agcgccagta agcgggcaaa aaagccgaat accagggtgg agataattac
    17341 cacatcatgg ttagaaacat agtgttgcag cgcgatgtac agcacggcgg aggcaaatga
    17401 gacaccggca tacaactctt tctggaatac cagtgggata cgtttacaga acatatcgcg
    17461 caaaacgccg ccaaacacgc cggtggttac cgccgcgaca acggcaataa ttgggccgtg
    17521 ccccatatcc agtgcaacct gtgcgccgat gatagaaaag accaccagtc caagcgcatc
    17581 gagcaccaga aacactttgc gcaggtaagg cattacagga gcaacgatag tggtgagtac
    17641 tgcggcggta gcgacgataa tcacatattc cgggtgtttg acccagccga gcggatagtg
    17701 gcccagcaga atatcgcgca ctgaccctcc gccaattgcg gtggcggtcg caataataat
    17761 tacgccaaat gtatccatgc ggcgtcgccc ggccgccaga gcccccgtca tggcttcggc
    17821 agtaatgcca accaaataca aaatgtgtaa cagcatggtt ctctccctaa aaatctgtct
    17881 ggcagggtag cgatttgtgc gtattgtcac gattgagatt ttttaaggtg agaggaaatt
    17941 gataacttgt agttatgtga aatcgctatt ttctgtagcg tagatgcatt cttctgactt
    18001 cttgtctgag aaaatgcata agaaaaaata ggttaaaatt gaggggtgga aaagaaaggt
    18061 gcaatgttta gccattgtgt tcaccgcacc tggactttgg cttacgcatc gggtatctca
    18121 atcaacaaaa agagttttta gtttctgcta tttcttctct gtaattttta cactgttttc
    18181 caactgcttg atgctgacct gcggggtcgg caactcctca ggcatggttc cgccaagttc
    18241 ctgaatggtt tgcctcacct tgctacccac atcaaagtgt gtggtattag cttgctgttt
    18301 tgaattcacg ccatcccgct tgagtttttc ttctgtttgg gtagctcgaa agagattagc
    18361 cgccagttct gttgaaccca tatgatccag gatcttctga ctctttttca gccccttcag
    18421 ttggtggatg gctttctgat ctaatccacc atacagcccc tggtaaccat gattctgaaa
    18481 tatggcgaaa tcagtagctg tcgctaccgc agcttgctgt gctgcctcaa ccaattgttt
    18541 gttatgttct ttcaactcat ttctcagaaa cagacgcttt tcgtcttcac gaagttgctt
    18601 aaatgcttca tcgtcagcaa gctcctgccg tcgggtctgg atagcaaaat aagtctgccc
    18661 tgccgcaatg accggtttcg cagggtcgcc gttttgtact accaggtaac aggcatagcg
    18721 ggagagatga acatcttcaa gttctcgttg agcccctgaa cctaatacaa ccattttggt
    18781 ggtttcaacg aaatggtcag atgcagcttg attgctggct tcgcacgctt gagtcgcgcg
    18841 ggccagtacc ttttgaaaat tgcgccagtc ccgataatcc agtaacggtg ccaattctct
    18901 agctgaccaa aattcttgcc cttcagtgcc ataatgccgt atttcttcaa aaggttgatg
    18961 atgttcgttc attgcccact ccatgtgata tcgattgcaa agaaagtaaa tctgtaactg
    19021 tatttatata cagttgtgag tatctcacgt tctcagtgtg ctgggcaaaa aaacatcaca
    19081 aaatctggag caggcacgca aaacagaatt gaagttgcgg ggaagaaaaa tgccgctctg
    19141 tttgagtggg cggcatttgt gaaggctgaa aagtaagaat tggcgttacg agttattcaa
    19201 tgttctgaat ctgctcgcgc atctgctcaa tcaacacttt cagctcaatc gcggagtttg
    19261 tcacttcggc attgatagac ttcgacgcaa gagtgttcga ctcgcggttg aattcctgca
    19321 tcataaagtc cagacgacga ccaacggctt cttttttctt cagaatgttg taggtctctt
    19381 tgacatgcgc ttccaggcgg tccagttctt cggcaacgtc aattcgttgt gccagcagaa
    19441 ccagctcctg ctccagacgg ttgttttcca actgaacctg agcatcttcc agcttcgcga
    19501 ccagacgctc acgctgccat tgcaggattt ccggcatatg ggcgcggact ttgaccactt
    19561 cggcggtgac gccttccaga cgctgctcga tcaatgcttt cagtgcctga ccttcggttt
    19621 cgcgcgcgac aataaagtcg tccagcgtac catccagcgc cgcgagaatt tcagcggcaa
    19681 tggcgtcaag atcctgctcc tgggctgcca tcacgcccgg ccagcgtaga atatcaaccg
    19741 ggttgatttc cccttcgtca ctctgcattt ttacccagtt cgcggcagtt accagctgtt
    19801 tagccagttt ttcgttgagg atcagctcac cttgcgcgct aacatctggc tcatagcgca
    19861 gggtacattc cactttaccg cgcgtcaggc gagaacgaat acgctcgcga acgacaggtt
    19921 caaggctacg gaactgctcc ggcagacgaa agtaagtttc cagataacgc tggtttaccg
    19981 agcgcatttc ccaggttgcg ctcccccatt cacccttgat ttcacgccgg gcgtaggcgg
    20041 tcatactgcg gatcatagac gttcctgttt ataaaaggag aggtggaagg attatagcca
    20101 tcgatgcctt gtcaggatag gaataaccgc cggaagtccg tataatgcgc agccacattt
    20161 gtttcaagcc ggagatttca atatgcgtcc agcaggccgt agcaataatc aggtgcgtcc
    20221 cgttaccctg actcgtaact atacaaaaca tgcagaaggc tcggtgctgg tcgaatttgg
    20281 cgataccaaa gtgttgtgta ccgcctctat tgaagaaggc gtgccgcgct tcctgaaagg
    20341 tcagggccag ggctggatca ccgcagagta cggcatgctg ccacgttcta cccacacccg
    20401 taacgctcgt gaagcggcga aaggtaagca gggtggacgc acaatggaaa tccagcgtct
    20461 gatcgcccgt gctcttcgcg cggcagtaga tttgaaagcg ctgggtgagt tcaccattac
    20521 gctggactgc gacgtgcttc aggctgatgg tggcacgcgt accgcgtcga ttacgggtgc
    20581 ctgcgtggcg ctggcagatg cgctacagaa gctggtggaa aacggcaagc tgaaaaccaa
    20641 cccgatgaaa gggatggtag ccgcagtttc tgtcggaatt gtgaacggcg aagcggtttg
    20701 cgatctggaa tacgttgaag actctgccgc agagaccgac atgaacgtag tgatgaccga
    20761 agacgggcgc atcattgaag tgcaggggac ggcagaaggc gagccgttca cccatgaaga
    20821 gctactcacc ttgttggctc tggcccgagg gggaatcgaa tccattgtag cgacgcagaa
    20881 ggcggcgctg gcaaactgat ttttaaggcg actgatgagt cgcctttttt ttgtctgtag
    20941 aaaagtaaga tgaggagcga aggcatgaaa ccatatcagc gccagtttat tgaatttgcg
    21001 cttggcaagc aggtgttaaa gtttggcgag tttacgttga agtccgggcg caaaagcccc
    21061 tatttcttca acgccgggct gtttaatacc gggcgcgatc tggcactgtt aggccgtttt
    21121 tacgctgaag cgttggtgga ttccggcctt gagttcgatc tgctgtttgg ccctgcttac
    21181 aaagggatcc cgattgccac cactaccgcc gtggcgctgg cggagcatca tgaccttgac
    21241 ctgccgtact gctttaaccg caaagaggca aaagaccacg gtgaaggcgg caatctggtt
    21301 ggtagcgcgt tacaaggacg cgtaatgctg gtagatgatg tgatcaccgc cggaacggcg
    21361 attcgcgaat cgatggagat tattcaggct aatggcgcga cgcttgctgg cgtgttgatt
    21421 tcgcttgatc gtcaggaacg ggggcgcggc gagatttcgg cgattcagga agttgagcgt
    21481 gattacaact gcaaagtgat ctctatcatc accctgaaag acctgattgc ttacctggaa
    21541 gagaagccgg aaatggcgga acatctggcg gcggttaagg cctatcgcga agagtttggc
    21601 gtttaaagaa actcgccgga tgaaaagtca tccggcgtca tattactgca actgtgccgc
    21661 aattagcggc cagcgggcgt caaaatcatc cgtcgggcgg tatttaaatt cgctgcggac
    21721 aaaacgtgac agcatacctt cacagaaggc taggatctgg cttgccagca gggtttcatc
    21781 ggtggtgtaa ccttcaccct cacgcattct cttttcacgc aatacctgac gcagctgcgc
    21841 ttcaatacgc tcgaacagct ggttgatgcg cccttgcagg cgatcctgtt caaacattag
    21901 cgcatgacca gtgaggatgc gggtcaggcc aggattacgc tcaccaaaac cgagaagcag
    21961 caacacaatc agacgcaggc gcgctgtggt gtctttctca tctttcagaa tcagattgat
    22021 gcgagtaatc aggctatctt cgataaactc aatcaggcta tcgaacatgc gggtcttact
    22081 ggggaagtgg cgatacagtg ccgcttcgga aacgccgaca gaggcggcca gttttgccgt
    22141 cgtgatacgt tggcttccat cgctggattc cagcatcagc gccagagact gaagtatttc
    22201 ctcgcgacgg ttccttttcg cagtttgttt ttctgccatg ttacaaaata cccctgaaaa
    22261 taagcacttg ccaggcggca cccacgctat gaccgcaaac gaaatgttta cggctatgtt
    22321 atgacgttat tcggatgcgt atgtgttact gacgaccaga gtgaccaaag ccgccttcac
    22381 cgcggtcggt ggcgtcgaaa tcttccacca gattaaattc agcctgtact accggaacaa
    22441 aaatcatctg ggcgatacgt tcgccaggtt gaatggtgaa gctgtcctga ccacggttcc
    22501 acacggaaat catcaactgg ccctgatagt cggaatcgat caatcctacc aggttaccaa
    22561 gcacgatacc gtgcttatgt cccaatccgg agcgcggcag catcattgcc gccagtgaag
    22621 gatcggcaat atgaatcgcc agcccggtcg gaaccagcgt agtgtcaccc ggagccagtt
    22681 ctacggcgtc gtcgagacag gcacgcaggt caagtccggc agagccagag gtggcataag
    22741 tcgggagcgg aaattccttc ccaacgcgcg ggtccagaat cttaacgtcg atttttttca
    22801 tcataacggg tcacgatctc gtcgagtaat aattggccaa ggagctcttt gcgctcaagc
    22861 ggtaagactt tatctccgtc ctgccagaaa aggtgtaatg cgttgttgtc gctgttaaat
    22921 ccttgagttg gctgggaaac atcgttcgcg cagatcagat caaggttttt acggatacgt
    22981 ttttgccggg cgtattcttc cacattattt gtttcggcgg caaatccaac gacgtagggt
    23041 cgatggtctt ttagtgcggc aacgcctgcg acgatatcgg ggtttttaac catttttatt
    23101 gttaattcat caccctgcgt ggcctgcttt ttgattttct ctggggccac ggtagctgcg
    23161 cgataatccg ccacggcggc gcagccgata aaaatatttt gctgctgtac agaagcattc
    23221 acggcggctt ccatttccag cgcggtcatc acatcaacac gtttaacaaa cggtggcgtc
    23281 ggtagtgaaa ccggacctga taccagcgtg acgttcgcgc cacgacgggc agcggcggcg
    23341 gcgatagcaa aacccatctt gccggagctg tgattagaga tataacgcac gggatcgagc
    23401 ggttcacgcg tcgggccggc ggtaatcata atgttcagat gtttcaggtc gttgacgggc
    23461 gaaaaatgcg ctaccgccat atccacaatg gttaacggat cgagcattcg cccaggaccg
    23521 atatcaccac aagcctgact gccactgtct ggcccccaga tgagcaaacc acgggaagca
    23581 agcacctcta aattatgctg cgtggcagcg gcacggtaca tctgctggtt catggcgggg
    23641 agcacggcta caggcgcagg tgtagccaga caaatcgtcg ataccaggtc attcgccatt
    23701 ccggcagcaa cacgggcaat caaatctgcc gtggcagggg cgagaatcac taaatcagcc
    23761 catttaccca gctcaatatg gcccatagcg gcttcggctg ccgggtccag caaactgtcg
    23821 gaaacgggat aaccagaaac cgcctgcaag ctaagtgggg tgataaaggc ttttgccgct
    23881 tcggtcatgg ctacgcggac gtcggccccg cgatcgcgca aacgacgcac cagttcaggg
    23941 gttttatagg cagcaatacc gccgctaacg ccgagaacga tttttttacc ggccaggctc
    24001 atgatgattt ttcctgttgg gtgacaccag aagttggcga ttttatcaca atacttttgt
    24061 tgtcgtgcct tcacctgaga ttcactttgc gaggcgcttt ccaggattga aaactggccg
    24121 tcgatttaac ggaacggcta tgacaggatg cgagcaccac aaaggaggtg aaggtgaaaa
    24181 acaatgcaca gctgttgatg ccgcgcgaaa aaatgctgaa gtttggtatt agcgccttaa
    24241 ctgatgtcga gctgctggcg ctatttctgc gtaccggaac gcgcggtaaa gatgtattaa
    24301 ccctggcaaa agagatgctg gagaatttcg gctctcttta tggcttgtta acctctgaat
    24361 atgaacaatt tagtggtgtt catggaattg gcgtagcgaa atttgcccag ttaaagggga
    24421 ttgctgaact ggcgcgacgt tactacaacg tgcggatgcg tgaagaaagc cctttactca
    24481 gcccggagat gacgcgggaa tttttacaaa gccagctcac gggtgaggag cgggagatct
    24541 ttatggtgat ctttctcgac tcccaacacc gggttataac gcatagccgt cttttttccg
    24601 gcacgctaaa ccatgttgaa gtccatcctc gggaaattat ccgcgaagcg ataaaaataa
    24661 acgcctcggc gctgatcctt gcacataatc acccttcggg ttgtgctgaa cccagtaaag
    24721 cggataaact cattactgaa cggataataa agagttgtca gttcatggat ttacgcgtgc
    24781 tcgaccatat cgtgattggg cgtggagagt atgtttcttt tgccgaacgc ggctggattt
    24841 aacccgctat gcgcgatcct tcgggatctt tgtctgttcg ggacttgagc acatcgctga
    24901 gtcagcgtat actacgccac ctttgagaat ctcgggtttg gcatttgggc ctggcaatcg
    24961 agagttcaca tagaactgcg atgaccgggc tgtaaagcct gacgaggcgc caatacccca
    25021 tacgaagctc gagctaattt gatttttgga gaatagacat gtcccgagtc tgccaagtta
    25081 ctggcaagcg tccggtgacc ggtaacaacc gttcccacgc actgaacgcg actaaacgcc
    25141 gtttcctgcc gaacctgcac tctcaccgtt tctgggttga gagcgagaag cgttttgtca
    25201 ccctgcgcgt atctgctaaa ggtatgcgtg taatcgataa aaaaggcatc gatacagttc
    25261 tggctgaact gcgtgcccgt ggcgaaaagt actaagtact tagaggaaat aaatcatggc
    25321 taaaggtatt cgtgagaaaa tcaagctggt ttcttctgct ggtactggtc acttctatac
    25381 cactacgaag aacaaacgta ctaagccgga aaaactggaa ctgaaaaaat tcgatccagt
    25441 tgttcgccag cacgtgatct acaaagaagc gaaaatcaaa taattctcgc tttgatgtaa
    25501 caaaaaacct cgctccggcg gggttttttg ttatctgctt gcccccatat tgactgcatc
    25561 tgttcattcc tggagatgct atgcctgaat tacccgaagt tgaaaccagc cgccgcggca
    25621 tagaaccgca tctcgttggt gcaaccattc ttcatgcagt ggtgcgcaac ggacgcttgc
    25681 gctggccggt ttcagaagag atctaccgtt taagcgacca accagtgctt agcgtgcagc
    25741 ggcgggctaa atatctgctg ctggagctgc ctgagggctg gattatcatt catttaggga
    25801 tgtctggcag cctgcgcatc cttccagaag aacttccccc tgaaaagcat gaccatgtgg
    25861 atttggtgat gagcaacggc aaagtgctgc gctacaccga tccgcgccgc tttggtgcct
    25921 ggctgtggac caaagagctg gaagggcata atgtgctggc ccatcttgga ccggagccgc
    25981 ttagcgacga tttcaatggt gagtatctgc atcagaagtg cgcgaagaaa aaaacggcga
    26041 ttaaaccgtg gctgatggat aacaagctgg tggtaggggt agggaatatc tatgccagcg
    26101 aatcactgtt tgcggcgggg atccatccgg atcggctggc gtcatcactg tcgctggcag
    26161 agtgtgaatt gttagctcgg gtgattaaag cggtgttgct gcgttcgatt gagcagggtg
    26221 gtacaacgct gaaagatttt ctgcaaagtg atggtaaacc gggctatttc gctcaggaat
    26281 tgcaggttta cgggcgaaaa ggtgagccgt gtcgggtgtg cggtacgccg attgtggcga
    26341 ctaaacatgc gcagcgggca acgttttatt gtcggcagtg ccagaagtaa ttcatgcgcg
    26401 ccggatggca taccatccgg cataaacgct acgctaactt cgccatcagc gcctgatgga
    26461 cattctccgg caggaaatgg gtgacatcgc cctgatggcg cgccacctct ttcaccaacg
    26521 atgaagagat aaacgaccac tctttcgacg gcatcagaaa cacactttcc agttccggca
    26581 ttaagtggcg attcatatgc gccagctgca tttcatattc aaaatctgcc accgcacgca
    26641 ggccacgaat cagcaccgta gcgtgttgat tacgggcgaa gttcgccatc aaatcactaa
    26701 acccgaccac ttccacgttc cccagatgcg cggttgcctg ctgtgccagt gccacacgct
    26761 cttccagggt aaacatcggt tttttactgg ggctggcggc aatcgccaga ataacgtgat
    26821 cgaacatctg cgtggcgcgc gtcacgatat cgatatgacc attggtaatg ggatcgaaag
    26881 tacccggata aatcgcccgt ttttgcataa caacctcaat gcgttttcgg tggcaggtaa
    26941 ggttccagca gttgaagcag acgctgtagc gcgccctggt tttgatacag tacttcaacg
    27001 gcatgacggc catagaaact acggtaatcg gcgtcggtga gtaaagagga aacctctttt
    27061 gcaagcgtag tggcatcggt aacggtaatc agcccgcttg cctgctccag ccgcgcgcaa
    27121 atgtctttaa agttaaaagt atgcggcccc atcaataccg gaattgcgtg tgcggcagct
    27181 tccagcggat tatgcccacc acgttcaacc agtgaaccgc caacaaaggc gagatcggca
    27241 atgccgtaca gtaacatcaa ctcgcccatc gtatcgccaa ccacaacctg cgtgctggtg
    27301 gaggggactt cccctgaaga gcgtgtgata tagcttagtc cagcctggcg gacaaggtta
    27361 atcgcatccg ggaagcgttc tggatgacgg ggtaccagga tgagcaataa attcgggaat
    27421 tgctgtaata aagcctgatg tgcggcaatg accacgctct cttcaccttc gtgagtgctg
    27481 gtggcaatcc ataccgggcg gtgtggtgcc cactggcggc gcagcgtcac ggctttagca
    27541 gccaactgcg gcgttacaga aatatcgaat ttcaggctac cggtaacggt tacctggtta
    27601 tttttcgcgc ccagcgccac aaaacgtgca ccatcttctt cattttgggc ggcaatcagc
    27661 gtaatacgac gcagcaagcg acggacgaat ttacccagtt tggcataacc tgcggccgag
    27721 cgggcagaga gtcgcgcgtt agcgatcacc agcggaattt tacgtttatg tagcgccgca
    27781 atcaggttag gccatagttc ggtttccata atcaacacca gtttagggtc gactttattc
    27841 aggaaacggt tgagtgcatc gggcagatca tacggcagat aaacgtgctg aacatccttc
    27901 ccgaaagccg attgtacgcg ctccgaaccg gttggcgtca tggttgttac ggtaataggt
    27961 aaatcaggat aacgatgacg cagcgcgcgc accaacggga tcgccgccag agtttcaccg
    28021 acggagacgg agtgcagcat aatgccgcct ggttttagcg gatggcggta aaaaccgtaa
    28081 cgttcacccc agcgttttcg ataggccgga gccttacgtc cgcgcaccca gagccgtatc
    28141 cagatcagcg gctgaataag gtagagaagg gcggtgtaaa gcaattcgag catagtaaat
    28201 agctgactta tggatgtgct ggggattcta tgtatttagc tgtggcttta ccattacttt
    28261 tcccgttttt gacttaaata gcaatttacc cgccaattct tgctgaacat ctatactttt
    28321 tttgtatttt tatgcgattc attgatttct gctgtacgtg caggaaggaa tcgcagagca
    28381 acagtgaaag aatatggttg ctaatgattt tatttgattg atttctttat tgaaaatcaa
    28441 caatgtgctt aaaaatcatt cgggtcggat tgccgcgaaa gaagtgatac actagcgcgt
    28501 caatatcagt gcggtatcag tattcaggta gctgttgagc ctggggcggt agcgtgcttt
    28561 ttctgcttca ccttaatcgg ataatctcaa caaaaagagt tacttgtgga taagccattt
    28621 cgaagaattt tgctcattaa gatgcgtttt catggggata tgttattaac tactcccgtc
    28681 attagttcgc tgaaaaaaaa ttaccctgac gcaaaaatcg atgtgctgct ttatcaggac
    28741 accatcccga tcctgtctga aaatccagag attaacgcgc tctacggcat aaaaaataaa
    28801 aaagcaaaag cctcagaaaa aattgccaac ttttttcatc tcatcaaggt attacgtgcc
    28861 aataagtatg accttatcgt caatcttacc gatcaatgga tggttgctat actggttcgc
    28921 ttattaaatg cccgtgtgaa aatttcccag gattatcatc atcggcagtc tgctttttgg
    28981 cgtaaaagtt tcacccattt ggtgccgttg cagggtggaa atgtggtgga aagtaactta
    29041 tccgtgctga caccattggg acttgattcg ttggtgaagc agacaaccat gagttacccg
    29101 cctgcaagct ggaaacgtat gcgtcgcgaa cttgatcacg ctggtgttgg acaaaattat
    29161 gtggttatcc aacctacggc gcggcaaatc ttcaaatgct gggacaacgc caagttttcc
    29221 gctgtgattg atgccttaca tgctcgtggt tatgaagtcg ttctgacgtc cggcccagat
    29281 aaagacgatc tggcctgcgt caatgaaatt gcgcaaggat gccagacgcc accagtaacg
    29341 gcgctggctg gaaaggtgac cttcccggaa cttggtgcgt taatcgatca tgcgcagctg
    29401 tttattggcg ttgattccgc accggcgcat attgccgctg cagttaatac gccgctgata
    29461 tcgctgtttg gtgcgacaga ccatattttc tggcgtcctt ggtcaaataa catgattcaa
    29521 ttctgggcgg gagattaccg ggaaatgcca acgcgcgatc agcgtgaccg aaatgagatg
    29581 tatctttcgg ttattccggc ggcagatgtc attgctgctg tcgataaatt actgccctcc
    29641 tccacgacag gtacgtcgtt atgatcgttg ctttttgttt atataaatat tttccctttg
    29701 gcggtttgca gcgcgatttt atgcgtattg ctcagacagt cgccgcccga ggtcatcatg
    29761 ttcgggttta tacccagtcg tgggaaggcg aatgccctga tgtatttgaa ctgatcaaag
    29821 tgccggttaa atcgcatacc aatcacgggc gcaatgcgga gtattttgcc tgggtgcaaa
    29881 aacatttacg cgaacatccc gtcgataaag tcgttggatt caacaaaatg ccggggctgg
    29941 acgtttatta tgccgctgat gtttgttatg ccgagaaagt cgcgcaggaa aaaggctttt
    30001 tctatcacct gacgtcacgt tatcgccatt atgccgcctt tgagcgggca accttcgaac
    30061 agggcaagcc gacacagctg ctgatgctga cagataagca aatcgccgat ttccagaaac
    30121 attatcagac tgaagcggag cgttttcata ttctaccacc ggggatttat cctgatcgta
    30181 aatatagcca gcagccagct aatagccgtg aaatcttccg taagaagaat ggaataaccg
    30241 aacaacaata tttattgttg caggtcggtt cagacttcac gcgtaaaggt gtcgatcgtt
    30301 ccattgaagc acttgcttcg ttaccggatt cgctgcgcca caacacattg ctatatgttg
    30361 ttggacagga taaaccgcga aaatttgagg cgctggcaga aaaacgcggc gtgcgcagta
    30421 atgttcactt cttctcgggg cgcaacgatg tctcggaatt aatggcggcg gcggatttat
    30481 tactgcatcc tgcctaccag gaagcagcgg gaattgtgct actggaagcg attactgcag
    30541 gattaccggt actaacaaca gccgtttgtg gctatgcgca ttatattgtc gacgccaatt
    30601 gcggcgaggc tattgctgag ccattccgcc aggaaacatt gaatgagatt ttacgcaaag
    30661 cgttaacgca atcttcattg cgccaggcct gggcggaaaa tgcgcgacat tatgctgata
    30721 cacaagattt atacagtctg ccagagaaag cggcggatat cataacgggt ggtctggatg
    30781 gttgaactta aagagccgtt tgccacgtta tggcgcggta aagatccttt tgaggaagtt
    30841 aaaaccttgc agggtgaggt ttttcgtgaa ctggaaactc gccgcactct gcgatttgaa
    30901 atggcgggca aaagctattt tctcaaatgg catcgcggca cgaccctgaa agagataatc
    30961 aaaaatttac tctcattgcg gatgccagta ttaggcgcag accgcgaatg gaatgcgatt
    31021 catcgactgc gggatgtcgg cgttgatact atgtatgggg tggcatttgg cgaaaaaggc
    31081 atgaatccgc tgaccagaac ttcatttatt attaccgaag atctgacacc aaccataagt
    31141 cttgaagatt actgtgctga ctgggcgact aaccctccag atgttcgcgt aaagcgtatg
    31201 cttattaagc gtgtcgcgac gatggtgcac gatatgcatg ctgcgggcat taaccaccgt
    31261 gactgttata tctgtcattt cctgctgcac ttgccttttt ccggtaagga agaggagtta
    31321 aaaatttcgg taattgacct gcaccgggcg cagcttcgca cgcgcgttcc acgtcgttgg
    31381 cgggataaag atcttattgg gctttatttt tcttcgatga atatcggcct gactcagcgg
    31441 gatatctggc ggtttatgaa agtgtatttt gccgccccgc ttaaagacat tctcaagcag
    31501 gaacaaggac tgctgtcgca agcagaagca aaagccacaa aaatcaggga aagaacgatt
    31561 cgaaaatcgt tgtaacccgt aacttatttt tgccaaaatt ttggatacag aataaatatg
    31621 tctcaactca atgatagtga catcatcctt tttgagtata attttcatta tcaaaatata
    31681 agatctaaaa atactcttga tatagcattt ggtattgaca gaaattttct t
//
LOCUS       DBFIFM010000036        31127 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.036, whole genome shotgun sequence.
ACCESSION   DBFIFM010000036 DBFIFM010000000
VERSION     DBFIFM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.036"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..546
                     /locus_tag="N7C74_001442"
     CDS             <1..546
                     /locus_tag="N7C74_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HGU2431340.1"
                     /translation="PIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCT
                     GVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKD
                     EGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGL
                     RFAIREGGRTVGAGVVAKVLS"
     gene            776..1159
                     /gene="secE"
                     /locus_tag="N7C74_001443"
     CDS             776..1159
                     /gene="secE"
                     /locus_tag="N7C74_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HGU2431341.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1161..1706
                     /gene="nusG"
                     /locus_tag="N7C74_001444"
     CDS             1161..1706
                     /gene="nusG"
                     /locus_tag="N7C74_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HGU2431342.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1865..2293
                     /gene="rplK"
                     /locus_tag="N7C74_001445"
     CDS             1865..2293
                     /gene="rplK"
                     /locus_tag="N7C74_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HGU2431343.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2297..3001
                     /gene="rplA"
                     /locus_tag="N7C74_001446"
     CDS             2297..3001
                     /gene="rplA"
                     /locus_tag="N7C74_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HGU2431344.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3293..3790
                     /gene="rplJ"
                     /locus_tag="N7C74_001447"
     CDS             3293..3790
                     /gene="rplJ"
                     /locus_tag="N7C74_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HGU2431345.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3857..4222
                     /gene="rplL"
                     /locus_tag="N7C74_001448"
     CDS             3857..4222
                     /gene="rplL"
                     /locus_tag="N7C74_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HGU2431346.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4542..8570
                     /gene="rpoB"
                     /locus_tag="N7C74_001449"
     CDS             4542..8570
                     /gene="rpoB"
                     /locus_tag="N7C74_001449"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HGU2431347.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8647..12870
                     /gene="rpoC"
                     /locus_tag="N7C74_001450"
     CDS             8647..12870
                     /gene="rpoC"
                     /locus_tag="N7C74_001450"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HGU2431348.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            13083..13526
                     /locus_tag="N7C74_001451"
     CDS             13083..13526
                     /locus_tag="N7C74_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="HGU2431349.1"
                     /translation="MKQEVEKWRPFKHPDGDVRDLSFLDAHQVIYVQHHEDKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKLSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVVFRAFSEIFHHCLKLIEK"
     gene            complement(13961..15094)
                     /gene="thiH"
                     /locus_tag="N7C74_001452"
     CDS             complement(13961..15094)
                     /gene="thiH"
                     /locus_tag="N7C74_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HGU2431350.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDERRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(15091..15861)
                     /gene="thiG"
                     /locus_tag="N7C74_001453"
     CDS             complement(15091..15861)
                     /gene="thiG"
                     /locus_tag="N7C74_001453"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HGU2431351.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLAPQSGPGSRSYFAHATSPLTGFLEASV"
     gene            complement(15863..16063)
                     /gene="thiS"
                     /locus_tag="N7C74_001454"
     CDS             complement(15863..16063)
                     /gene="thiS"
                     /locus_tag="N7C74_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HGU2431352.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLVQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(16047..16802)
                     /gene="thiF"
                     /locus_tag="N7C74_001455"
     CDS             complement(16047..16802)
                     /gene="thiF"
                     /locus_tag="N7C74_001455"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HGU2431353.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWGQGCYRCLWPENQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16795..17430)
                     /gene="thiE"
                     /locus_tag="N7C74_001456"
     CDS             complement(16795..17430)
                     /gene="thiE"
                     /locus_tag="N7C74_001456"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGU2431354.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLSDYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17430..19325)
                     /gene="thiC"
                     /locus_tag="N7C74_001457"
     CDS             complement(17430..19325)
                     /gene="thiC"
                     /locus_tag="N7C74_001457"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HGU2431355.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTQPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGIADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19406..19506)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19558..20034)
                     /gene="rsd"
                     /locus_tag="N7C74_001458"
     CDS             complement(19558..20034)
                     /gene="rsd"
                     /locus_tag="N7C74_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HGU2431356.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20129..20902
                     /gene="nudC"
                     /locus_tag="N7C74_001459"
     CDS             20129..20902
                     /gene="nudC"
                     /locus_tag="N7C74_001459"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HGU2431357.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRYDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKHLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20942..22006
                     /gene="hemE"
                     /locus_tag="N7C74_001460"
     CDS             20942..22006
                     /gene="hemE"
                     /locus_tag="N7C74_001460"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HGU2431358.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVALYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            22016..22687
                     /gene="nfi"
                     /locus_tag="N7C74_001461"
     CDS             22016..22687
                     /gene="nfi"
                     /locus_tag="N7C74_001461"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HGU2431359.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22730..23320
                     /gene="yjaG"
                     /locus_tag="N7C74_001462"
     CDS             22730..23320
                     /gene="yjaG"
                     /locus_tag="N7C74_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HGU2431360.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREADESNIGIIFQQ"
     gene            23507..23779
                     /gene="hupA"
                     /locus_tag="N7C74_001463"
     CDS             23507..23779
                     /gene="hupA"
                     /locus_tag="N7C74_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HGU2431361.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23792..24487
                     /gene="yjaH"
                     /locus_tag="N7C74_001464"
     CDS             23792..24487
                     /gene="yjaH"
                     /locus_tag="N7C74_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HGU2431362.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHFLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24489..24914)
                     /gene="zraP"
                     /locus_tag="N7C74_001465"
     CDS             complement(24489..24914)
                     /gene="zraP"
                     /locus_tag="N7C74_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HGU2431363.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25152..26528
                     /gene="zraS"
                     /locus_tag="N7C74_001466"
     CDS             25152..26528
                     /gene="zraS"
                     /locus_tag="N7C74_001466"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HGU2431364.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEVWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANCREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASLEGKGARFTLWLPVN
                     ITRKDPQG"
     gene            26525..27850
                     /gene="zraR"
                     /locus_tag="N7C74_001467"
     CDS             26525..27850
                     /gene="zraR"
                     /locus_tag="N7C74_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HGU2431365.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHIIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMLVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LAQSLDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27847..29136)
                     /gene="purD"
                     /locus_tag="N7C74_001468"
     CDS             complement(27847..29136)
                     /gene="purD"
                     /locus_tag="N7C74_001468"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HGU2431366.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPTLQ
                     NVAIGVTDIPALLDFAQNEKVDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMASEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVEDGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29148..30737)
                     /gene="purH"
                     /locus_tag="N7C74_001469"
     CDS             complement(29148..30737)
                     /gene="purH"
                     /locus_tag="N7C74_001469"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HGU2431367.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7312 a         8054 c         8523 g         7238 t
ORIGIN      
        1 ccgatcgaag acgtattctc catctccggt cgtggtaccg ttgttaccgg tcgtgtagaa
       61 cgcggtatca tcaaagttgg tgaagaagtt gaaatcgttg gtatcaaaga gactcagaag
      121 tctacctgta ctggcgttga aatgttccgc aaactgctgg acgaaggccg tgctggtgag
      181 aacgtaggtg ttctgctgcg tggtatcaaa cgtgaagaaa tcgaacgtgg tcaggtactg
      241 gctaagccgg gcaccatcaa gccgcacacc aagttcgaat ctgaagtgta cattctgtcc
      301 aaagatgaag gcggccgtca tactccgttc ttcaaaggct accgtccgca gttctacttc
      361 cgtactactg acgtgactgg taccatcgaa ctgccggaag gcgtagagat ggtaatgccg
      421 ggcgacaaca tcaaaatggt tgttaccctg atccacccga tcgcgatgga cgacggtctg
      481 cgtttcgcaa tccgtgaagg cggccgtacc gttggcgcgg gcgttgtagc aaaagttctg
      541 agctaattgc cgataacatt tgacgcaatg cgcactaaaa gggcatcatt tgatgccctt
      601 tttgcacgct ttcgtaccag aacctggctc atcagtgatt ttctttgtca taatcattgc
      661 tgagacaggc tctgttgagg gcgtataatc cgaaaagcta atacgcgttt cgatttggtt
      721 tgcctcgcga tcgcggggtg aaaatgtttg tagaaaactt ctgacaggtt ggtttatgag
      781 tgcgaatacc gaagctcaag gaagcgggcg cggcctggaa gcgatgaagt gggtcgttgt
      841 ggtggcattg ctcttggtgg cgattgtcgg caactatctt tatcgcgaca ttatgctgcc
      901 gctgcgtgcg ctggccgtag taattctgat tgctgcagcg ggtggtgtcg cgctgttaac
      961 gacaaaaggt aaagctaccg ttgcttttgc ccgtgaagcg cgtaccgaag tccgtaaggt
     1021 catttggccg actcgccagg aaacattgca caccacgctg attgtggctg cggttaccgc
     1081 agtaatgtca ctgatcctgt ggggactgga tggtattctg gttcgcctgg tatcctttat
     1141 cactggcctg aggttctgag atgtctgaag ctcctaaaaa gcgctggtac gtcgttcagg
     1201 cgttttccgg ttttgaaggc cgcgtagcaa cgtcgctgcg tgagcatatc aaattacaca
     1261 acatggaaga tttgtttggt gaagtcatgg taccaaccga agaagtggtt gaaatccgtg
     1321 gcggtcagcg tcgcaaaagc gaacgtaaat tcttccctgg ctacgtcctc gttcagatgg
     1381 tgatgaacga cgcgagctgg cacctggtgc gcagcgtacc gcgtgtgatg ggcttcatcg
     1441 gcggtacttc cgatcgtcct gcgccaatca gcgataaaga agtcgatgcg attatgaacc
     1501 gcctgcagca ggttggtgat aagccgcgtc cgaaaacgct gtttgaaccg ggtgaaatgg
     1561 tccgtgttaa tgatggtccg ttcgctgact tcaacggtgt tgttgaagaa gtggattacg
     1621 agaaatctcg tctgaaagtg tctgtttcta tcttcggtcg tgcgaccccg gtagagctgg
     1681 acttcagcca ggttgaaaaa gcctaaccca gcgatcaaaa aagcggcgat ttaatcgttg
     1741 cacaaggcgc gagattggaa tacaatttcg cgccttttgt ttttatgggt cttgcccgta
     1801 aaacgatttt ttatatcacg gggagcctct aagaggcgtt attacccaac ttgaggaatt
     1861 tataatggct aagaaagtac aagcctatgt caagctgcag gttgcagctg gtatggctaa
     1921 cccgagtccg ccagtaggtc cggctctggg tcagcagggc gtaaacatca tggaattctg
     1981 caaagcgttc aacgcaaaaa ctgattccat cgaaaaaggt ctgccgattc cggtagtaat
     2041 caccgtttac gctgaccgtt ctttcacttt cgttaccaag actccgccgg cagcagttct
     2101 gctgaaaaaa gcggctggta tcaagtctgg ttccggtaag ccgaacaaag acaaagtggg
     2161 taaaatttcc cgcgctcagc tgcaggaaat cgcgcagacc aaagctgccg acatgactgg
     2221 tgccgacatt gaagcgatga ctcgctccat cgaaggtact gcacgttcca tgggcctggt
     2281 agtggaggac taagaaatgg ctaaactgac caagcgcatg cgtgttatcc gcgagaaagt
     2341 tgatgcaacc aaacagtacg acatcaacga agctatcgct ctgctgaaag agctggcgac
     2401 tgctaaattc gtagaaagcg tggacgtagc tgttaacctc ggcatcgacg ctcgtaaatc
     2461 tgaccagaac gtacgtggtg caactgtact gccgcacggt actggccggt ccgttcgcgt
     2521 agccgtattt acccagggtg caaacgctga agctgctaaa gctgcaggtg ctgaactggt
     2581 aggtatggaa gatctggctg accagatcaa gaaaggcgaa atgaactttg acgttgttat
     2641 tgcttctccg gatgcaatgc gcgttgttgg ccagctgggc caggttctgg gtccgcgcgg
     2701 cctgatgcca aacccgaaag tgggtactgt aacgccgaac gttgctgaag cggttaaaaa
     2761 cgctaaagct ggccaggttc gttaccgtaa cgacaaaaac ggcatcatcc acaccaccat
     2821 cggtaaagtg gactttgacg ctgacaaact gaaagaaaac ctggaagctc tgctggttgc
     2881 gctgaaaaaa gcaaaaccga ctcaggcgaa aggcgtgtac atcaagaaag ttagcatctc
     2941 caccaccatg ggtgcaggtg ttgcagttga ccaggctggc ctgagcgctt ctgtaaacta
     3001 atgcctttac gtgggcggtg attttgtcta caatcttacc cccacgtatg ctaacgaaag
     3061 ttagcagcta aaaagatttg ttcgttggag cctggcctat ccaggcctcc gtcgaagacc
     3121 gcaggagttt cgcaagaaac ttaatcccct gcgtagacgg tgacagaacg ctaagattat
     3181 tcttttatat tctggcttgt ttctgctcac cgtaattaag acgctctctc cgtttggagg
     3241 agtgaagtga gttccagaga ttttctctgg caaacatcca ggagcaaagc taatggcttt
     3301 aaatcttcaa gacaaacaag cgattgttgc tgaagtcagc gaagtagcca aaggcgcgct
     3361 gtctgcagta gttgcggatt cccgtggcgt aactgtagat aaaatgactg aactgcgtaa
     3421 agcaggtcgc gaagctggcg tatacatgcg tgttgttcgt aacaccctgc tgcgccgtgc
     3481 tgttgaaggt actccgttcg agtgcctgaa agacgcgttt gttggtccga ccctgattgc
     3541 atactctatg gaacacccgg gcgctgctgc tcgtctgttc aaagagttcg cgaaagcgaa
     3601 tgcaaaattt gaggtcaaag ccgctgcctt tgaaggtgag ctgatcccgg cgtctcagat
     3661 cgaccgcctg gcaactctgc cgacctacga agaagcaatt gcacgcctga tggcaaccat
     3721 gaaagaagct tcggctggca aactggttcg tactctggct gctgtacgcg atgcgaaaga
     3781 agctgcttaa tcgcagttat ctttttaacg cattcgctta cgtataaact tattctgata
     3841 ttcaggaaca atttaaatgt ctatcactaa agatcaaatc attgaagcag ttgcagctat
     3901 gtctgtaatg gacgttgtag aactgatctc tgcaatggaa gaaaaattcg gtgtttccgc
     3961 tgctgctgct gtagctgtag ctgctggccc ggttgaagct gctgaagaaa aaactgaatt
     4021 cgacgtaatt ctgaaagctg ctggcgctaa caaagttgct gttatcaaag cagtacgtgg
     4081 cgcaactggc ctgggtctga aagaagctaa agacctggta gaatctgcac cggctgctct
     4141 gaaagaaggc gtgagcaaag acgacgcaga agcactgaaa aaagctctgg aagaagctgg
     4201 cgctgaagtt gaagttaaat aagccaaccc ttccggttgc agcctgagaa atcaggctga
     4261 tggctggtga ctttttagtc accagccttt ttgcgctgta aggcaccagt agcgtttcac
     4321 actgtttgac tactgctgtg cctttcaatg cttgtttcta tcgacgactt aatatactgc
     4381 gacaggacgt ccgttctgtg taaatcgcaa tgaaatggtt taagcgtgat agcaacaggc
     4441 attgcggaaa gtgttccatt ttccggtcaa caaaatagtg ttgcacaaac tgtccgctca
     4501 atggacagat gggtcgactt gtcagcgagc tgaggaaccc tatggtttac tcctataccg
     4561 agaaaaaacg tattcgtaag gattttggta aacgtccaca agttctggat gtaccttatc
     4621 tcctttctat ccagcttgac tcgtttcaga aatttatcga gcaagatcct gaagggcagt
     4681 atggtctgga agctgctttc cgttccgtat tcccgattca gagctacagc ggtaattccg
     4741 agctgcaata cgtcagctac cgccttggcg aaccggtgtt tgacgtccag gaatgtcaaa
     4801 tccgtggcgt gacctattcc gcaccgctgc gcgttaaact gcgtctggtg atctatgagc
     4861 gcgaagcgcc ggaaggcacc gtaaaagaca ttaaagaaca agaagtctac atgggcgaaa
     4921 ttccgctcat gacagacaac ggtacctttg ttatcaacgg tactgagcgt gttatcgttt
     4981 cccagctgca ccgtagtccg ggcgtcttct ttgactccga caaaggtaaa acccactctt
     5041 cgggtaaagt gctgtataac gcgcgcatca tcccttaccg tggttcctgg ctggacttcg
     5101 aattcgatcc gaaggacaac ctgttcgtac gtatcgaccg tcgccgtaaa ctgcctgcga
     5161 ccatcattct gcgtgccctg aactacacca cagagcagat cctcgacctg ttctttgaaa
     5221 aagttatctt tgaaatccgt gataacaagc tgcagatgga actggtgccg gaacgcctgc
     5281 gtggtgaaac cgcatccttt gacatcgaag ctaacggtaa agtgtacgta gaaaaaggcc
     5341 gccgtatcac tgcgcgccac attcgccagc tggaaaaaga cgacgtcaaa ctgatcgaag
     5401 tcccggttga gtacatcgca ggtaaagtgg ttgctaaaga ctatattgat gagtctaccg
     5461 gcgagctgat ctgcgcagcg aacatggagc tgagcctgga tctgctggct aagctgagcc
     5521 agtctggtca caagcgtatc gaaacgctgt tcaccaatga tctggatcac ggcccgtata
     5581 tctctgaaac cttacgtgtc gacccaacta acgaccgtct gagcgcactg gtagaaatct
     5641 accgcatgat gcgccctggc gagccgccga ctcgtgaagc agcggaaagc ctgttcgaga
     5701 acctgttctt ctccgaagac cgttatgacc tgtctgcggt tggtcgtatg aagttcaacc
     5761 gttctctgct gcgcgaagaa atcgaaggtt ctggtatcct gagcaaagac gacatcattg
     5821 atgttatgaa aaagctcatc gatatccgta acggtaaagg cgaagtcgat gatatcgacc
     5881 acctcggcaa ccgtcgtatc cgttccgttg gcgaaatggc ggaaaaccag ttccgcgttg
     5941 gcctggtacg tgtagagcgt gcggtgaaag agcgtctgtc tctgggcgat ctggataccc
     6001 tgatgcctca ggatatgatc aacgccaagc cgatttccgc agcagtgaaa gagttcttcg
     6061 gttccagcca gctgtctcag tttatggacc agaacaaccc gctgtctgag attacgcaca
     6121 aacgtcgtat ctccgcactc ggcccaggcg gtctgacccg tgaacgtgca ggcttcgaag
     6181 ttcgagacgt acacccgact cactacggtc gcgtatgtcc aatcgaaacc cctgaaggtc
     6241 cgaacatcgg tctgatcaac tctctgtccg tgtacgcaca gactaacgaa tacggcttcc
     6301 ttgagactcc gtatcgtaaa gtgactgacg gtgttgtaac tgacgaaatt cactacctgt
     6361 ctgctatcga agaaggcaac tacgttatcg cccaggcgaa ctccaacctg gatgaagaag
     6421 gccacttcgt agaagacctg gtaacctgcc gtagcaaagg cgaatccagc ttgttcagcc
     6481 gtgaccaggt tgactacatg gacgtatcca cccagcaggt ggtatccgtc ggtgcgtccc
     6541 tgatcccgtt cctggaacac gatgacgcca accgtgcatt gatgggtgcg aacatgcaac
     6601 gtcaggccgt tccgactctg cgtgctgata agccgctggt tggtactggt atggaacgtg
     6661 ctgttgccgt tgactccggt gtaactgcgg ttgctaaacg tggtggtgtc gttcagtacg
     6721 tggatgcttc ccgtatcgtt atcaaagtta acgaagacga gatgtatccg ggtgaagcag
     6781 gtatcgacat ctacaacctg accaaataca cccgttctaa ccagaacacc tgtattaacc
     6841 agatgccgtg tgtgtctctg ggtgaaccgg ttgaacgtgg cgacgtgctg gcagacggtc
     6901 cgtccaccga ccttggtgaa ctggcgcttg gtcagaacat gcgcgtagcg ttcatgccgt
     6961 ggaatggtta caacttcgaa gactccatcc tcgtatccga gcgtgttgtt caggaagacc
     7021 gtttcaccac catccacatt caggaactgg cgtgtgtgtc ccgtgacacc aagctggggc
     7081 cagaagagat caccgctgac atcccgaacg tgggtgaagc tgcgctctcc aaactggatg
     7141 aatccggtat cgtttatatt ggtgcggaag tgaccggtgg cgacattctg gttggtaagg
     7201 ttacgccgaa aggtgaaact cagctgaccc cagaagaaaa actgctgcgt gcgatcttcg
     7261 gtgagaaagc gtctgacgtt aaagactctt ctctgcgcgt accaaacggt gtatccggta
     7321 cggttatcga cgttcaggtc tttactcgcg atggcgtaga aaaagacaaa cgtgcgctgg
     7381 aaatcgaaga aatgcagctc aaacaggcga agaaagacct gtctgaagaa ctgcagatcc
     7441 tcgaagcggg tctgttcagc cgtatccgtg ctgtgctggt agccggtggc gttgaagctg
     7501 agaagctcga caaattgccg cgcgatcgct ggctggagct gggcctgacc gacgaagaga
     7561 aacaaaatca gctggaacag ctggctgagc agtatgacga actgaaacac gagttcgaga
     7621 agaaactcga agcgaaacgc cgcaaaatca cccagggcga cgatctggca ccgggcgtgc
     7681 tgaagattgt taaggtatat ctggcggtta aacgccgtat ccagcctggt gacaagatgg
     7741 caggtcgtca cggtaacaag ggtgtaattt ctaagatcaa cccgatcgaa gatatgcctt
     7801 acgatgaaaa cggtacgccg gtagacatcg tactgaaccc gctgggcgta ccgtctcgta
     7861 tgaacatcgg tcagatcctc gaaacccact tgggtatggc tgcgaaaggt atcggcgaca
     7921 agatcaacgc catgctgaaa cagcagcagg aagtcgcgaa actgcgtgaa ttcatccagc
     7981 gtgcgtacga tctgggcgct gacgttcgtc agaaagttga cctgagtacc ttcagcgatg
     8041 aagaagttat gcgtctggct gaaaacctgc gcaaaggtat gccaatcgca acgccggtgt
     8101 tcgacggtgc gaaagaagca gaaattaaag agctgctgaa acttggcgac ctgccgactt
     8161 ctggtcagat ccgcctgtac gacggccgca ctggtgaaca gttcgaacgt ccggtaaccg
     8221 ttggttacat gtacatgctg aaactgaacc acctggtcga cgacaagatg cacgcgcgtt
     8281 ccaccggttc ttacagcctg gttactcagc agccgctggg tggtaaggca cagttcggtg
     8341 gtcagcgttt cggggagatg gaagtgtggg cgctggaagc atacggcgca gcatacaccc
     8401 tgcaggaaat gctcaccgtt aagtctgatg acgtgaacgg tcgtactaag atgtataaaa
     8461 acatcgtgga cggcaaccat cagatggagc cgggcatgcc agaatccttc aacgtattgt
     8521 tgaaagagat tcgttcgctg ggtatcaaca tcgaactgga agacgagtaa ttctcgctca
     8581 aacaggtcac tgctgtcggg gtaaaacccg gcagcggatt gtgctaactc cgacgggagc
     8641 aaatccgtga aagatttatt aaagtttctg aaagcgcaga ctaaaaccga agagtttgat
     8701 gcgatcaaaa ttgctctggc ttcgccagac atgatccgtt catggtcttt cggtgaagtt
     8761 aaaaagccgg aaaccatcaa ctaccgtacg ttcaaaccag aacgtgacgg ccttttctgc
     8821 gcccgtatct ttgggccggt aaaagattac gagtgcctgt gcggtaagta caagcgcctg
     8881 aaacaccgtg gcgtcatctg tgagaagtgt ggcgttgaag tgacccagac taaagtacgc
     8941 cgtgagcgta tgggccacat cgaactggct tccccgactg cgcacatctg gttcctgaaa
     9001 tcgctgccgt cccgtatcgg cctgctgctc gatatgccgc tgcgcgatat cgaacgcgta
     9061 ctgtactttg aatcctatgt ggttatcgaa ggcggtatga ccaacctgga gcgtcagcag
     9121 atcctgactg aagagcagta tctggacgcg ctggaagagt tcggtgacga attcgacgcg
     9181 aaaatgggtg cggaagcaat ccaggccctg ctgaagagca tggatctgga gcaagagtgc
     9241 gaacagctgc gtgaagagct gaacgaaacc aactccgaaa ccaagcgtaa aaagctgacc
     9301 aagcgtatca aactgctgga agcgttcgtt cagtctggta acaaaccaga gtggatgatc
     9361 ctgaccgttc tgccggtact gccgccagat ctgcgtccgc tggttccgct ggatggtggt
     9421 cgtttcgcga cttctgacct gaacgatctg tatcgtcgcg tcattaaccg taacaaccgt
     9481 ctgaaacgtc tgctggatct ggctgcgccg gacatcatcg tacgtaacga aaaacgtatg
     9541 ctgcaggaag cggtagacgc cctgctggat aacggtcgtc gcggtcgtgc gatcaccggt
     9601 tctaacaagc gtcctctgaa atctttggcc gacatgatca aaggtaaaca gggtcgtttc
     9661 cgtcagaacc tgctcggtaa gcgtgttgac tactccggtc gttctgtaat caccgtgggt
     9721 ccatacctgc gtctgcatca gtgcggtctg ccgaagaaaa tggcgctgga gctgttcaaa
     9781 ccgttcatct acggcaagct ggaactgcgt ggtcttgcta ccaccattaa agctgcgaag
     9841 aaaatggttg agcgcgaaga agctgtcgtt tgggatatcc tggacgaagt tatccgcgaa
     9901 cacccggtac tgctgaaccg tgcaccgact ctgcaccgtc tgggtatcca ggcatttgaa
     9961 ccggtactga tcgaaggtaa agctatccag ctgcacccgc tggtttgtgc ggcatataac
    10021 gccgacttcg atggtgacca gatggctgtt cacgtaccgc tgacgctgga agcccagctg
    10081 gaagcgcgtg cgctgatgat gtctaccaac aacatcctgt ccccggcgaa cggcgaacca
    10141 atcatcgttc cgtctcagga cgttgtactg ggtctgtact acatgacccg tgactgtgtt
    10201 aacgccaaag gcgaaggcat ggtgctgact ggcccgaaag aagcagaacg tctgtatcgc
    10261 tctggtctgg cttctctgca tgcgcgcgtt aaagtgcgta tcaccgagta tgaaaaagat
    10321 gctaacggtg aattagtagc gaaaaccagc ctgaaagaca cgactgttgg ccgtgccatt
    10381 ctgtggatga ttgtaccgaa aggtctgcct tactccatcg tcaaccaggc gctgggtaaa
    10441 aaagcaatct ccaaaatgct gaacacctgc taccgcattc tcggtctgaa accgaccgtt
    10501 atttttgcgg accagatcat gtacaccggc tttgcatatg cagcgcgttc tggtgcatct
    10561 gttggtatcg atgacatggt catcccggag aagaaacacg aaatcatctc cgaggcagaa
    10621 gcagaagttg ctgaaattca ggagcagttc cagtctggtc tggtaactgc gggcgaacgc
    10681 tacaacaaag ttatcgatat ctgggctgcg gcgaacgatc gtgtatccaa agcgatgatg
    10741 gataacctgc aaactgaaac cgttattaac cgtgacggtc aggaagagaa gcaggtttcc
    10801 ttcaacagca tctacatgat ggccgactcc ggtgcgcgtg gttctgcggc acagattcgt
    10861 cagcttgctg gtatgcgtgg tctgatggcg aagccggatg gctccatcat cgaaacgcca
    10921 atcaccgcga acttccgtga aggtctgaac gtactccagt acttcatctc cacccacggt
    10981 gctcgtaaag gtctggcgga taccgcactg aaaactgcga actccggtta cctgactcgt
    11041 cgtctggttg acgtggcgca ggacctggtg gttaccgaag acgattgtgg tacccatgaa
    11101 ggtatcatga tgactccggt tatcgagggt ggtgacgtta aagagccgct gcgcgatcgc
    11161 gtattgggtc gtgtaactgc tgaagacgtt ctgaagccgg gtactgctga tatcctcgtt
    11221 ccgcgcaaca cgctgctgca cgaacagtgg tgtgacctgc tggaagagaa ctctgtcgac
    11281 gcggttaaag tacgttctgt tgtatcttgt gacaccgact ttggtgtatg tgcgcactgc
    11341 tacggtcgtg acctggcgcg tggccacatc atcaacaagg gtgaagcaat cggtgttatc
    11401 gcggcacagt ccatcggtga accgggtaca cagctgacca tgcgtacgtt ccacatcggt
    11461 ggtgcggcat ctcgtgcggc tgctgaatcc agcatccagg tgaaaaacaa aggtagcatc
    11521 aagctcagca acgtgaagtc ggttgtgaac tccagcggta aactggttat cacttcccgt
    11581 aacaccgaac tgaaactgat cgacgaattc ggtcgtacca aagaaagcta caaagtacct
    11641 tacggtgcgg tactggcaaa aggcgatggc gaacaggttg ctggcggcga aaccgttgca
    11701 aactgggacc cgcacaccat gccggttatc accgaagtaa gcggttttgt acgctttact
    11761 gacatgatcg acggccagac cattactcgt cagaccgacg aactgaccgg tctgtcttcg
    11821 ctggtggttc tggattccgc agaacgtacc gcaggtggta aagatctgcg tccggcactg
    11881 aaaatcgttg atgctcaggg taacgacgtt ctgatcccag gtaccgatat gcctgcgcag
    11941 tacttcctgc cgggtaaagc gattgttcag ctggaagatg gcgtacagat cagctctggt
    12001 gacaccctgg cgcgtattcc gcaggaatcc ggcggtacca aggacatcac cggtggtctg
    12061 ccacgcgttg cggacctgtt cgaagcacgt cgtccgaaag agccggcaat cctggctgaa
    12121 atcagcggta tcgtttcctt cggtaaagaa accaaaggta aacgtcgtct ggttatcacc
    12181 ccggtagacg gtagcgatcc gtacgaagag atgattccga aatggcgtca gctcaacgtg
    12241 ttcgaaggtg aacgtgtaga acgtggtgac gtaatttccg acggtccgga agcgccgcac
    12301 gacattctgc gtctgcgtgg tgttcatgct gtgactcgtt acatcgttaa cgaagtacag
    12361 gacgtatacc gtctgcaggg cgttaagatt aacgataaac acatcgaagt tatcgttcgt
    12421 cagatgctgc gtaaagctac catcgttaac gcgggcagct ccgacttcct ggaaggcgaa
    12481 caggttgaat actctcgcgt caagatcgca aaccgcgaac tggaagcgaa cggcaaagtg
    12541 ggtgcaactt actcccgcga tctgctgggt atcaccaaag cgtctctggc aaccgagtcc
    12601 ttcatctccg cggcatcgtt ccaggagacc actcgcgtgc tgaccgaagc agccgttgcg
    12661 ggcaaacgcg acgaactgcg cggcctgaaa gagaacgtta tcgtgggtcg tctgatcccg
    12721 gcaggtaccg gttacgcgta ccaccaggat cgtatgcgtc gccgtgctgc gggtgaagct
    12781 ccggctgcac cgcaggtgac tgcagaagac gcatctgcca gcctggcaga actgctgaac
    12841 gcaggtctgg gcggttctga taacgagtaa tcgttaatcc gcaaataacg taaaaacccg
    12901 cttcggcggg tttttttatg gggggagttt agggaaagag catttgtcag aatatttaag
    12961 gaatttctga atactcataa tcaatgtaga gatgactaat atcctgaaac tgactgaact
    13021 aattgagtca aactcggcaa ggattcgata ctattcctgt gtagctttct taaggaacga
    13081 gaatgaaaca ggaagtggag aagtggcgac cttttaaaca tccggatggc gacgttcgtg
    13141 atttatcgtt tcttgatgct catcaggtta tctacgttca gcatcatgag gacaaggagc
    13201 ctttagagta tcgcttttgg gttacctact ctcttcactg cttcacaaaa gattatgaac
    13261 atcagacgaa cgaagaaaaa ctatcgttaa tgtaccacgc gcctaaagaa tctcgtccct
    13321 tctgccagca ccgttataac ttagcgcgca cacacttaaa aagaactatt ttggcgctgc
    13381 cagaaagcaa cgttattcat gccgggtatg gtagctatgc cgtgattgag gtggacttag
    13441 acggaggaga taaggcattt tactttgttg tgttcagggc tttcagtgaa atttttcacc
    13501 attgcctaaa acttattgag aaataagcag cttcctcagc cctcaaaata acaaaaccca
    13561 ccttaaggtg ggtttcgcca gagaattatc tctggtattc agaacgccat taccggactt
    13621 tgccttgacc ttgcgataat cgcaggttgc gggatgtctg aatttcttca gtctgctgca
    13681 tcctggaaga tgagaacatg tgttcttatt ttcgtctcta tcatagttga gtatttactc
    13741 tcttacaatc agatctcttt cattgctcaa caggcgatgg cttcagactt tgcattacgg
    13801 aatttttaag aaaggcaggg cgaaacgagg aagaagcttt tcgccctgga caacattcct
    13861 gctgacggca ctaccataaa aaaacacaac gctttcaaca accttgcgtt tttcatcttc
    13921 cgtagcggct cggaataaat tttcgttctt acattccgtc tcatagtctt tgcgaggcgc
    13981 gtcccagata gctgtcccag tctttccata ccggctgcaa accctgagcg gttaacgcgg
    14041 cagcaaccgc ttccggtctg cgctcgtcgt gcggtgagaa ctgttccagc tcggggtgat
    14101 tgtcggcata gccacctggc tgcgttttcg aaaaagcgct gacgttatta attgccagcg
    14161 gaataacgcg atcgcgaaac cacggtgatt cccgcgtgga gagtgacagt tcaatctccg
    14221 gtgcaagcag ccggaaggcg cagatggttt gcactaactg gcgttcatcc ataatcgacg
    14281 caggctcaat gccgccagta catggacgca ggcgtgggaa ggagacagag tagcggcttt
    14341 gccagtaatg ctgttgcagc catagcaaat gttctgcaac catatagcag tcaacgcgcc
    14401 agctgtcgga aaggccaatt agcgcgccga ggcctatctt atcaatcccc gcacggccca
    14461 gccgatccgg cgtttccagc cgccagaaga agtcctgttt tttgcctttc agatgatggc
    14521 gggcataagt cgcctcgtga tatgtctcct gataaaccat cacgccatcc agaccaagtt
    14581 gctttaactc ggcgtattcc gtctccgcca gcggctgtac ttccatctgt agtgaagaga
    14641 actgttcacg cagggcaggg agatgacgac gaaagtaatc catccccact ttcgcctgat
    14701 gttcaccggt gactaaaagc agatgttcaa agcccatctc ccgtatagcg gcgctttccc
    14761 tggcaatatc cgcttcatcc agcgttttgc gcttgatgcg gttgctcata gagaatccgc
    14821 agtacgtgca gtcgttagcg caaagattgg aaagataaag cgggacgtag aaactaactg
    14881 tgttgccaaa tcgctgacgg gtcagacgct gcgcccgttg ggccagttgt tccagatagc
    14941 cactggcggc tggcgataac agcgccatca tatcgtcgcg ggtgagttgc gaggcattta
    15001 gcgcccgctc tacgtcagca gccgttttgc cgttgatacg caggcggatg tcgtcccagt
    15061 ccagttgtcg ccagcgatcg ctgaaggttt tcataccgat gcctccagaa atccggtcag
    15121 cgggctggtg gcatgagcaa aataactgcg gctgcccggt ccggactgag gtgccagcag
    15181 gcctgcttct actgccagac gaaatgcctt cgccatgttg acgggatcgt ccgcgacggc
    15241 aatcgccgta ttcactaaca ccgcgtcggc ccccatttcc agcgcctgcg cggcatggct
    15301 gggaacgccg atgccagcat cgacaaccac cggaactgtg gcctgctgga taataatctc
    15361 cagcatggcg cgggtttcca gcccctgatt cgagccaatc ggcgcgccga gcggcatcac
    15421 cgctgcacag ccgacttctt ccagacgttt acacaatacc ggatcggccc cgcagtaagg
    15481 cagcacgaca aatccctgtt gtaccagcgt ttcggcggct ttcagggttt cgatgggatc
    15541 gggcaacagc cagcgggcgt cagggtgaat ctccaatttt aaccagtttg tgcctaacgc
    15601 ttcacgagcc agatgggcgg cgaaaatggc ttcttccgct gttttcgccc cggatgtatt
    15661 tggcagcagg gtcacacccg ccgcgataag cggttcgagg atagcgtcgt tgtgctggcg
    15721 caagtcgaca cgtttcatcg ccagcgtcac cagctggctg ccggaagcgc ggattgcctc
    15781 taccatcagt tgtgaagagg cgaatttccc ggtgccggta aacagatgtg aatcaaacgt
    15841 tttgtccgca atacgtaaca tctcaacccc ctgcaataac ctgaaaaagt aggatctggt
    15901 cgccatcctg cacgatatgt tgcgtccact gctcacgcgg gacgatttgc tgattaatcg
    15961 ccagagccgc gcccgcttgt cgttgaacga gttgctccag tagttcgtga acagtttgcc
    16021 cggcggcgca ctgcatcggt tgatcgttaa acaggatctg cattgcttcc tccgcatacc
    16081 gggcaaccac tggcgcggcg caacgccagg ctgcgccact ggctcgactt accgtcgaac
    16141 agtcggagtt ctcccgcagg cgtctctata ccgcttaata acttaatggc ttccagtgcc
    16201 tgcaaagtgc ccataacccc gaccaccggg ccaaccacgc ccgccgtgcg gcagttgcgt
    16261 tctggctcct ggttttctgg ccacaggcag cggtaacaac cctgccccca gggcggcgtc
    16321 agtaccatca actgaccgcc aaatccgacc gcgctggcgg tgataagcgg cgtgttgagt
    16381 gccacgcagg cggcattaat ctcctggcga gtcgccatat tgtcggtaca gtcgagcacc
    16441 acatcggctt gtgcaaccgc atcttttaac gcctcacccg ttaaccgttg ttgtaatgct
    16501 gtcagttgaa tatcgggatt caactgtgtc agtcgctgat ggctgacctg cgatttcggg
    16561 cgatcgatat cttcagtggt aaagaggatt tgtcgttgca gattgcttaa atgcacatcg
    16621 tcgtcatctg ccagtaccag cgtcccgacg ccagcgcccg ccaggtatag cgcagcaggt
    16681 gtacccagcc cgccaagacc gataatcagc acctggctgt cgagcagttt ttgctgcccg
    16741 tcaagagcga tatcgtcgag caggatttgg cggctataac gcataaaatc acggtcattc
    16801 atcgccaact cctgcaattt ccagcaactg tgccgttgcc aaacgccagt ctgcggcttg
    16861 agtaatggcg ctgacgacgg cgatactgcc gacacccgtt gctatcaccg caggcgcgcg
    16921 tgccagactg ataccgccaa tcgccacggt gggataatcc gacagtcgct caacatgccg
    16981 tgccagctgt tccagcccct gcggtgcaga aggcatctgt ttggtttgcg tcgggaacac
    17041 atgtcccagc gcgatataag aggggcgtgc tgccagcgcg acgtcgattt ccatatcgtc
    17101 atgggtcgaa acgcccagcc gcaggcctgc cgcgcggatg gcattgagat cggtggcttg
    17161 caaatcttcc tgccccaaat ggacgccata cgcctgatgc ttgatcgcga gccgccagta
    17221 atcgttgata aacaatcgcg cgttatagcg gcggcccagc gcaattgccg ccacgacatc
    17281 ggcttccacc tcttcatcgc gccgatcttt gatgcgtagc tggagagtac gtacgcctgc
    17341 atccaacaga cgttcgatcc actgtacgct gtccaccacc gggtacagtc ctaaacgaaa
    17401 aggtacagga ggaaaatcag gctgatacat cacgcttcct ccttacgcag gtagatttct
    17461 ccgcctctgg cacggaagtt ctccgacata tccgctattc ccatttcaat agtttgcgtg
    17521 gcggcgtaat cacgcacttc ctggctgatt ttcatcgagc agaatttcgg cccacacatg
    17581 gagcaaaaat gggcgacttt acctgactct tgcggcaggg tttcatcgtg ataagcgcgg
    17641 gcggtaaacg ggtcgagggc cagattaaac tggtcttccc agcgaaattc gaagcgggct
    17701 ttcgacatgg cgttatcgcg aatttgtgcg cccggatgcc ctttagccag gtcagcggcg
    17761 tgggcggcaa tcttataggt gataagcccc tgcttaacat cttctttatt aggcagaccc
    17821 agatgctctt ttggcgttac gtaacagagc atcgcgcagc caaaccagcc aatcatcgcc
    17881 gcaccaatcc ccgacgtgaa gtggtcatag cccggcgcaa tatcggtagt tagcggcccc
    17941 agagtgtaaa acggcgcttc gtggcagtgc tctaactcct cggtcatatt gcggcggatc
    18001 atctgcatcg gcacgtggcc tgggccttca atcatcacct gcacatcata ttcccaggcg
    18061 atttttgtca gttcgcccag cgtatgcagc tcggcaaact gcgcttcatc gttggcgtcc
    18121 tgaatagaac cggggcgcag accgtcgccc agcgacagcg aaacgtcata agcggcacag
    18181 atttcacaaa tttcgcggaa gtgttgatag aggaaatttt cctgatgatg ggagaggcac
    18241 catttcgcca taatcgaacc gccgcgcgag acgataccgg tcaggcgttt cgcggtcatc
    18301 ggcacatagc gcagcagcac ccccgcatgg atagtgaagt aatccacacc ttgctcggcc
    18361 tgttccagca gcgtgtcgcg gaacgcttcc caggtaagat cttcggcgat cccattaacc
    18421 ttctccagcg cctggtagat cggcactgta ccaatcggca ccgggctgtt acgcaaaatc
    18481 cactcgcggg tttcgtgaat atagcgaccg gtggagagat ccatcaccgt atccgctccc
    18541 cagcgcgtgg accataccag cttttccact tcttcttcga tggaagaggt gaccgccgag
    18601 ttgccgatat tggcgttaac ttttaccagg aaattgcgac caataatcat cggctccgat
    18661 tccggatgat taatgttggc cgggataatc gcacgtccgg cagcaacttc atcacggaca
    18721 aattccgcag tgatattttc cggcagacgt gcgccaaagc tcattcccgg atgctggtgg
    18781 cgtaaaactt cgctacggat gcgctcgcgg cccatattct cgcggatggc gatgaattcc
    18841 atttccggtg tgatgatgcc ctggcgggcg tagtgcagtt gtgtgacgcg gcgtcctgct
    18901 ttggcgcgtt ttggcgttaa tacgccgcta aaacgcagct cgtcgaggcc atcatctgcc
    18961 agccgcgctt tagtgtaatc ggaactgcgc acggtaagtt cttcggtatc gccgcgcgca
    19021 tcgatccacg gctggcgtag ttttgccagc ccttgctgca cgttaatggc gatctgcgga
    19081 tcgccatacg ggccggaggt gtcgtagacc ggaatcgctt cgttttcttc gtactgcggc
    19141 tgttctttgc taccgccaat tagcgtcggg ctaagctgga tctcacgcat cggcacgcgc
    19201 acgccgggtt gtgtgccagt gatataaatg cgttttgagt tgggaaaggc ggtgccttcc
    19261 agggtgtcga taaaatgttg ggcctgggcg cgttgttcgc gacgggtcag ttttgttgca
    19321 gacatagctc attccaaaaa gttaaggacg tggcttgtca gacgacggat gaagcaagag
    19381 acgatcgccg caaaggcgat gcgatagcag attaactctt gttcccttcg caggtattag
    19441 cctgatcagg ttccgcggat cccgaataaa cggtctcagc cagttaaggc actccgacaa
    19501 gaaattagcc ccacaaacgg ggcgttgaat gtaaattacg cgttaacagc gcagaactca
    19561 agcaggatgt ttgacgcggg cggcgtcaag caccagcaga atcagcttat cttccagcac
    19621 aaagcgcgct tccagcgcct cgccgatgtc agataaaacc tgttgaaact caaggtaatt
    19681 atcatgatcg atagcggttt ccaggctgga atcgtagtaa tccataatct gttgggtatt
    19741 ggcttcgagt tgcggccaaa tcttcgcggc gcgcgcgagt tgcccgttgc cttccagctt
    19801 atgaagaatg cgctcataaa tactgaaatg tccggcagac aagtaatcga ccaggctctg
    19861 acaaaaatca tcaagggctt tttcgtttag cctcatgtac gattctttgc caggcttaat
    19921 gccaaccaga ttgtagtaag ccacgagcag atgcttacgt acatgtagcc agcgatcaac
    19981 cagtttgtta cttcctctga cgcgctccgt caggttatcg agctggttaa gcatgattga
    20041 ctccgcaagt ttgtattcaa aaactgctca gtgagaaatg taaaaaccat gttaaacatg
    20101 ccagtgatgc aaaggtagtg caagagctat ggatcgtata attgaaaaat tagatcacgg
    20161 ctggtgggtc gtcagccatg aacaaaaatt atggttgccg aagggagaat tgccatatgg
    20221 cgaagcggca aatttcgatc ttgtgggtca gcgcgcacta caaatcggcg aatggcaggg
    20281 ggaacctgtt tggttaatac aacagcagcg gcgttacgat atggggtcgg tacgtcaggt
    20341 cattgatctc gatgttgggc tgtttcaact ggccggacgt ggcgtacaac tggcggagtt
    20401 ttaccgatcg cataaatact gtggttactg cgggcatgaa atgtatccaa gcaaaaccga
    20461 atgggcgatg ctgtgcagcc attgccgtga gcgttactac ccgcaaatcg ccccctgcat
    20521 tattgttgcc atccgtcgcg atgattcgat cctcctcgcc cagcataccc gccatcgtaa
    20581 cggtgtccat acggtacttg ccggattcgt cgaagtgggc gaaaccctcg agcaggcggt
    20641 cgcgcgggaa gtgatggaag agagcggaat taaagttaaa cacttgcgtt atgtgacttc
    20701 ccagccgtgg ccgtttcctc agtctttaat gaccgcgttt atggcggaat atgacagcgg
    20761 cgacatcgtg atcgacccga aagaattgct cgaggcgaac tggtatcgct atgacgattt
    20821 gccgttactc ccgccgcccg gcaccgtagc gcgccgtctg atagaagata cggtggcgat
    20881 gtgtcgggca gagtatgagt gatgatacac tgaccgcctg acgcactaag gaacagccaa
    20941 aatgaccgaa cttaaaaacg atcgttatct gcgggcgctg ctgcgccagc ccgttgatgt
    21001 cactcctgta tggatgatgc gccaggcggg tcgctatcta ccggaatata aagccacgcg
    21061 cgcccaggcg ggcgatttta tgtcgctgtg caaaaacgcc gagctggcgt gtgaagtgac
    21121 cttgcaaccg ctgcgtcgct acccgctgga tgcggcgatc ctcttttccg atatcctcac
    21181 cgtcccggac gcgatggggt tagggctcta ttttgaagcc ggagaaggtc cgcgttttac
    21241 ctcgccagtc acctgcaaag ctgacgtcga taaactgcca attccggacc cggaagatga
    21301 actggggtac gtgatgaacg cggtgcgtac cattcgtcgc gaactgaaag gcgaagtgcc
    21361 gctgattggt ttttccggca gcccgtggac gctggcgacc tacatggtgg aaggcggcag
    21421 cagcaaagcc ttcaccgtga tcaaaaaaat gatgtatgcc gatccgcagg cgctgcacgc
    21481 tctactcgat aaactggcga aaagcgtcgc tttgtatctg aatgcgcaga ttaaagccgg
    21541 tgctcaggct gtgatgattt tcgacacctg gggcggcgtg cttaccgggc gcgattatca
    21601 acagttctcg ctttattaca tgcataaaat tgttgatggt ttactgcgtg aaaacgacgg
    21661 tcgccgcgta ccggtcacgc tgtttaccaa aggcggcgga cagtggctgg aagccatggc
    21721 agaaaccggt tgcgatgcgc tgggcctcga ctggacaacg gacatcgccg atgcgcgccg
    21781 ccgtgtgggc aataaagtcg cgttgcaggg taatatggat ccgtcgatgc tgtacgcgcc
    21841 gcctgcccgc attgaagaag aagtagcgac tatacttgca ggtttcggtc acggcgaagg
    21901 tcatgtcttt aaccttggtc acggcattca tcaggatgtg ccgccagaac atgctggcgt
    21961 gttcgtggag gcagtgcatc gactgtctga acagtatcac cgctaaggag cgattatgga
    22021 tctcgcgtca ttacgcgctc aacaaatcga actggcttct tctgtgatcc gcgaggatcg
    22081 actcgataag gatccgccgg atctgatcgc cggagccgat gtcgggtttg agcagggcgg
    22141 agaagtgacg cgagcggcga tggtgctgct gaaatacccc tcgcttgagc tggtggagta
    22201 taaagtggcc cgcatcgcta ccaccatgcc ttacattcca ggttttcttt ccttccgcga
    22261 atatcctgcg ctgctggcag cgtgggagat gctgtcacaa aagccggatt tagtgtttgt
    22321 cgatggtcat gggatctcgc atcctcgccg tcttggcgtc gccagccatt ttggtttgct
    22381 ggtggatgtg ccgaccattg gcgtagcgaa aaaacgacta tgcggtaaat tcgaaccgct
    22441 ctccagcgaa ccgggcgcgc tggccccact gatggataaa ggcgagcagc tggcctgggt
    22501 ctggcgcagc aaagcgcgct gtaacccgct atttatcgct actggtcatc gggtcagcgt
    22561 ggacagcgcg ctggcgtggg tacaacgctg catgaaaggc tatcgtctgc cggagccaac
    22621 gcgctgggcg gacgcggtgg cctcggaacg tccggcgttc gtgcgctata cagcaaatca
    22681 gccctaattc aggtaaactg cggataattt ccgtatttga gaactcaaca tgttacaaaa
    22741 cccaattcat ctgcgtctgg aacgcctgga aagctggcag catgtcactt tcatggcttg
    22801 cttatgcgaa cgcatgtacc ccaattacgc catgttctgc cagcaaaccg gttttggtga
    22861 tgggcaaatt taccgtcgta ttctcgatct catctgggaa acgctgaccg ttaaagatgc
    22921 aaaagtaaat ttcgacagcc aactggagaa atttgaagaa gcgattcctt cagccgacga
    22981 tttcgatctg tacggcgttt atccggcaat cgatgcctgc gtggcgttaa gtgaactggt
    23041 ccattcgcgt ttgagtggtg aaacgctcga acacgcggtg gaagtgagta agacctccat
    23101 cacgaccgtt gcgatgctgg aaatgactca ggctggtcgc gaaatgagcg atgaagagct
    23161 caaagaaaac ccagctgtag agcaagaatg ggacattcag tgggaaatat tccgactttt
    23221 agctgagtgc gaagaacgcg atatcgagct gataaaaggc cttagggcag acctgcgtga
    23281 ggcggatgag agcaatattg gtataatttt tcagcaataa gaccagaaaa cgtgatttaa
    23341 cgcctgattt gtcgtacctg gagtcttccc tttcgccccc cgtctggtct acatttgggg
    23401 ggcgaaaaaa agtggctatc ggtgcgtgta tgcaggagag tgctattctg gcatttccgt
    23461 cgcactcgat gcttagcaag cgataaacac attgtaagga taacttatga acaagactca
    23521 actgattgat gtaattgcag agaaagcaga actgtccaaa acccaggcta aagctgctct
    23581 ggagtccact ctggctgcaa ttactgagtc tctgaaagaa ggcgatgctg tacaactggt
    23641 tggtttcggt accttcaaag tgaaccaccg cgctgagcgt actggccgca acccgcagac
    23701 cggtaaagaa atcaaaattg ccgcagctaa cgtaccggca tttgtttctg gcaaggcact
    23761 gaaagacgca gttaagtaag attgcgtggc agtgaacagt tttaacgaag gggtggtttc
    23821 accccttttg tctttctggc gtcgatcatt gatgctggct ggcgctctgt ttctcactgc
    23881 ttgtagtcac aactcttcac ttcctccctt taccgccagt ggatttgctg aagaccaggg
    23941 cgcggtacgc atctggcgaa aagacagcgg cgataatgtg cattttcttg ccgtgtttag
    24001 cccgtggcgc agtggcgata ccacgacgcg agagtatcgc tggcagggcg ataacctcac
    24061 gctcatcaat atcaatgttt acagcaaacc cccggtgaat attcgcgcgc gttttgacga
    24121 tcgcggtgat ctgagcttta tgcaacgtga atccgatggg gaaaagcagc agctttctaa
    24181 cgaccaaatc gatttatacc gttatcgggc tgatcagatc cgccagatta gcgatgcctt
    24241 acgtcagggg agagtcgtgc tgcgccaggg gcgctggcat gcgatggaac agaccgtgac
    24301 cacctgcgaa gggcaaacca ttaaacctga tttagattcg caggcaatag cgcatatcga
    24361 gcgccgccag agccgctctt ctgttgatgt cagcgtggca tggctggaag cgcccgaagg
    24421 ttcgcaatta ctgttagtgg caaactctga tttctgtcgc tggcaaccca acgagaaaac
    24481 gttctgattt accagtggcc catacccata tgaccgccac caccgcagcc gccgtagccc
    24541 atgcccattc cggctccgcg cggaataccc gcttcagcca tcgcaatatc ccgtttcacc
    24601 cgtaactcat ctaacgactg acgcaaattc tccatctctt tggcgaccgc gttaattttg
    24661 ctgctatccg gtgggttcgc ggctaacagg gcattgtatt cataacgctt cgtcaccagt
    24721 tgctgttgca gtgcgctgct ttgagcgtaa aagtcattat ggattttctg ccacgctgtc
    24781 tgttgttcgc tggtcaaagg cgcggcattt tgctgccaca taccgtgtcc gccgtgagca
    24841 aatgcagatg tcgatcccat cgccattgct gaaagcgcca tcattaccag ggcaattttc
    24901 gtgttccgtt tcatggttaa tcctccagtg gttgtcttac ttcgggtatt gcatcttccg
    24961 tgccaacgat gaaacgctga tatgacgggt aatctggcat gataaacgag taaaaatgac
    25021 tcgcctgctg cgggtagcga gtcattttta ctcattgaaa cttaagcctt tgtgttacag
    25081 cgcagggtaa gcgctgataa aagatggcat gatttctgct gtcagaaagg gatgagcagg
    25141 caaagaagaa gatgcgtttt atgcaacgtt ctaaagactc cttagctaaa tggttaagcg
    25201 cgatcctccc cgtggtcatt gttgggctgg tagggttgtt tgcggtgacg gtgattcgcg
    25261 attatggccg cgagactgcc gccgccagac aaacgctgct ggaaaaaggc agtgtactta
    25321 ttcgtgctct cgaatccggc tcgcgcgtcg gcatggggat gcgcatgcat catgcgcagc
    25381 agcaggcctt actggaagaa atggccgggc agcctggagt acgttggttt gcggtcacgg
    25441 atgaacaagg aacaatcgtg atgcatagca actccggcat ggtgggaaaa cagctttatt
    25501 ccccgcagga aatgcagcag ttacatccgg gagatgaaga agtgtggcgg cggatcgata
    25561 gcgcagacgg cgagcctgtt ctggaaattt atcgccagtt tcaaccgatg tttgctgctg
    25621 gaatgcaccg gatgcgccat atgcagcagt atgccgcgac accacaagca attttcatcg
    25681 ctttcgacgc cagtaatatt gtgagtgccg aagatcgtga gcagagaaac accctgatta
    25741 tcctcttcgc cctggcgacg gtcttgctgg caagcgtatt gtcattcttc tggtatcgcc
    25801 gctatctgcg ctcgcgccag cttctacaag atgaaatgaa gcgcaaagag aagctggtgg
    25861 cgctggggca tcttgcggca ggcgttgccc acgaaattcg taacccgctt tcctcgatta
    25921 aagggctggc aaaatacttt gccgagcgcg cgccagcagg gggagaagcg catcaactgg
    25981 cgcaggtgat ggcgaaagaa gctgaccgtt taaaccgcgt ggtaagcgag ctgctggaac
    26041 tggttaagcc aacgcatctg gctttgcagg cggtggatct caacacgctg attaaccact
    26101 cattacagct ggtaagccag gatgcaaact gccgggagat ccagttacgc tttaccgcca
    26161 acgacacgtt gccggaaatt caggccgatc cggacaggct gactcaggtc ctgttgaatc
    26221 tttatctcaa tgctattcag gcgattggtc agcatggcgt gattagcgtg acggccagcg
    26281 aaagcggcgc gggcgtgaaa atcagcgtta ccgacagcgg taagggaatt gcggcagatc
    26341 agcttgaagc catcttcacc ccgtacttca ccaccaaagc cgaaggcacc ggactggggc
    26401 tggcggtcgt gcataatatt gttgaacaac acggtggtac aattcaggtc gcaagcctgg
    26461 agggaaaagg cgcaaggttc accctctggc ttccggtcaa tattacgcgt aaggacccac
    26521 aaggatgacg cacgataata tcgatattct ggtggtggat gatgacatta gccactgcac
    26581 tattttgcag gctttgctgc gcggctgggg ctataacgtc gcgctggcga acagcgggcg
    26641 acaggcgctg gagcaggtgc gggagcgggt ttttgatctt gtgctttgcg atgtgcgaat
    26701 ggcggagatg gacggcattg ccacgctaaa agagatcaaa gcgttaaatc cggcgattcc
    26761 ggtactgatt atgaccgcat actctagcgt cgagacggcg gtagaggcgt tgaaaaccgg
    26821 ggcgctggat tatctcatca agccgctgga tttcgataac ctgcaggcga cgctggaaaa
    26881 ggcgctcgcg catacgcaca ttattgatgc tgaaacgcct gcggtgactg ccagccagtt
    26941 cggtatggtc ggtaaaagcc cggcgatgca acacctgctc agtgaaatcg ccctcgtcgc
    27001 gccatcggaa gccacggtgc tgatccacgg cgattccggc accggtaaag agctggtcgc
    27061 cagggcgatt cacgccagta gcgcacgtag cgaaaaaccg ctggtaacgc tcaactgtgc
    27121 ggcactcaac gaatccttgc tggaatctga attgttcggt cacgaaaaag gggcgtttac
    27181 tggagccgat aaacgccggg aagggcgctt tgttgaggcg gacggcggca cgttgtttct
    27241 cgatgaaatt ggcgatatct cgccgatgat gctggtgcgt ctgctgcgtg cgattcagga
    27301 gcgcgaagtt cagcgtgtcg gtagcaacca gactatttcg gttgatgtcc ggctgattgc
    27361 cgcgacccat cgcgatcttg ccgcagaggt gaatgccggg cgttttcgcc aggatctcta
    27421 ctatcgcctg aacgtggtgg cgattgaagt accatcgctg cggcaacggc gggaagatat
    27481 tcctctgctg gctggccatt ttctgcagcg ctttgctgaa cgtaatcgca aggcggtaaa
    27541 aggctttacg ccccaggcga tggatctgtt gattcattac gactggccgg gaaatattcg
    27601 tgaactggaa aatgcggtgg aacgggcggt ggtgctgctg actggggaat atatttccga
    27661 acgcgagctg ccgctggcga ttgccagtac gccgatcccg ctggcacaaa gcctggatat
    27721 tcagccgctg gtggaagtcg aaaaagaagt gattctggcg gcactggaga aaacgggcgg
    27781 caacaaaacc gaagccgccc gtcagttagg gatcacgcgc aaaacgctgt tggcaaaatt
    27841 gtcgcgttag ttctgctcgc gttcgatagc gcgccagccg atatctttcc ggcagaagca
    27901 gtcgtcccag tggatatcgg tcattaaggc ataggcgcgt ttctgtgctt ctgctacggt
    27961 atgacccagc gcggtgacgc acagtacgcg cccgccgctg gttaccacct gctcgtcatc
    28021 cgccagtttt gtgcccgcgt ggaacacttt gccgtcttcc acttcttcca gcggcaggcc
    28081 gtggatcacg tcaccggtgc ggtaatcgcc cggatatcca cccgcagcca tcaccacgcc
    28141 gagagaagca cgttcatccc actctgacgt tttctcgtct agtttgcctt cacaggccgc
    28201 caggcagagt tcaacaagat cggacttcat gcgcagcata atcggctggg tttctggatc
    28261 gccaaagcgg cagttaaatt cgataacctt cggattgccc tgtttgtcga tcatcaggcc
    28321 cgcgtagaga aaaccggtgt aggtgttgcc ttccgacgcc atgcctttca cggttggcca
    28381 gatgatacgt tccatcgtgc gctgatgaac gtcatctgtc actaccggcg caggagagta
    28441 agcgcccatt ccgcccgtgt tcggcccggt atctttatcg cctacgcgtt tgtgatcctg
    28501 gctggtggcc atcggcagca catgctcgcc gtccaccatc acgataaagc tcgcttcttc
    28561 gccgtcgagg aactcttcga taacgatgcg atgacccgcg tcgccaaacg cgttgcccgc
    28621 cagcatatcg tgaacagccg cttccgcttc ttccagcgtc atcgccacga taacgccttt
    28681 cccggcagcc agaccgtccg ctttaatgac gattggcgcg cctttctcac gcagatacgc
    28741 cagcgcaggt tctacctcgg tgaagttctg atattccgcc gtaggaatgt tatggcgggc
    28801 caggaaatct ttagtaaacg ctttcgagcc ttccagctgt gcggcgcctg cggttgggcc
    28861 gaagattttc atcccggcgg cgcggaaggt atcgaccacg cctttcacca gcggcgcttc
    28921 cgggccgacg atggttagat ctaccttttc gttttgcgcg aaatccagca gcgccgggat
    28981 atcggtcacg ccgatagcga cgttttgcag cgtgggttcc agcgctgtgc ctgcgttacc
    29041 cggagcaaca aaaacagtct caaccagcgg cgactgggcc gctttccagg ccagcgcgtg
    29101 ctcgcgcccg ccgttaccaa tcactaatac tttcatctgt cgctccatta atggcggaag
    29161 tggcgcatat cggtgaagag catcgcaata ccgtgctcgt cggcggcggc aatcacttcg
    29221 tcatcacgga tggaaccgcc aggctggatt acgcaggtca cgcccgcagc ggcggcggca
    29281 tcaataccgt cgcggaacgg gaagaacgcg tcagaagcca tcgaggaacc tttcacttcc
    29341 aggccttcat cggcagcttt aataccggcg attttcgcgg agtacacgcg gctcatctgg
    29401 cccgcgccaa taccgatggt catattgttt ttggcataga cgatagcatt ggatttcacg
    29461 aacttcgcca ctttccagca gaacagcgca tcacgcagtt cctgttcggt cggctgacgt
    29521 ttggtgacga cgcgcagttc ttccgcaccg accatgccca gatcgcgatc ctgaaccagc
    29581 agaccgccgt tcacgcgttt gaaatcaaga cccggaacac gctcgcccca ctgaccgcag
    29641 gtcagaacgc gtacattctg tttggcggcg gtgattttca gggcttcttc gctggcggac
    29701 ggcgcaataa tcacttcaac aaactgacga gaaatgatgg cctgtgcggt ttccgcatcc
    29761 agctcgcggt taaaggcaat gatgccgccg aatgcggagg tcgggtcggt tttgtacgcg
    29821 cgatcgtaag catcaagaat tgaattgccg atagccacgc cgcaagggtt ggcgtgcttc
    29881 acaatcacac atgccggctc ggcgaactct ttcacgcact ccagcgccgc atcggtatcg
    29941 gcgatgttgt tataagagag ggctttaccc tgaacctggg ttgcggtagc aacggaggct
    30001 tctttcacat tctcttctat atagaaggca gcctgctggt ggctgttctc gccgtaacgc
    30061 atatcctgct tcttaatgaa gttcaggttc agcgtgcgtg ggaagcgacc ggcggcttct
    30121 ttgctttcac cgtgataagc cggaaccatg ctgccgaagt agttggcaat catgctgtcg
    30181 taggcggcag tgtgttcgaa ggctttgatg gcgaggtcga aacgggttgc aagcgtcagc
    30241 gatccttcgt tgtcatccat ctctttaata atggcgtcgt agtcgctgct cttcaccacg
    30301 attgcgacat ctttatggtt cttggcggcg gagcgcacca tcgttgggcc gccgatatcg
    30361 atgttctcaa ccgcatcttc cagcgagcaa ccttcacggg ccacggtctg ggcgaacgga
    30421 tacaggttaa caaccaccat atcgataggc tggatctgat gttcttccat aatggcatcg
    30481 tcctggccgc gacggcccag aatgccacca tgtactttcg gatgcagggt cttcacgcgt
    30541 ccatccatca tctccgggaa accggtgtaa tcggaaactt cggttaccgg cagacctttt
    30601 tctgctaaca gacgggcagt gccccctgta gacagcagct ccacaccgcg tgcggaaagt
    30661 gcctgggcga attcgacgat accggctttg tcagaaacac tgagcagagc gcggcggact
    30721 ggacgacgtt gttgcatggt aaatcccctg gatttgacta ttacagagag cgttagctga
    30781 atttttcgcg aaaaactcag ctaacgcccc taacggggca tccttatttt tcgcccgcat
    30841 tgtaacgaaa acgtttgcgc aacgctcgcg aattttttta attcgatggc gatcacgatt
    30901 ttgcccccga ttagcgtggg agaaatagag aaataacgct gatgattgtg gataactctg
    30961 tgcgtaaaaa ggtataaagc ggggttttgc tggggaatgc agcagtcagt catttttctg
    31021 caatttttct attgcggcct gcggagaact ccctataatg cgcctccatc gacacggcgg
    31081 atgtgaatca cttcacacaa acagccggtt cggttgaaga gaaaaat
//
LOCUS       DBFIFM010000037        30920 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.037, whole genome shotgun sequence.
ACCESSION   DBFIFM010000037 DBFIFM010000000
VERSION     DBFIFM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.037"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..569
                     /locus_tag="N7C74_001470"
     CDS             <1..569
                     /locus_tag="N7C74_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HGU2431368.1"
                     /translation="NLSGCGYNDIQTYDEQVNASWSEVLNQYQRRADLIPNLVASIKG
                     YSSHERDVLEAVTLARSQANRASSDLQQTPGDEQKLQAWQQAQAQVTRTLGQLTIISE
                     RYPELKSQELYQNLMVQLEGSENRIAVARGRYIKAIEQYNVTIRKFPAVLTAKVMDYT
                     PKKNYLPDDVAAVSKAPTIDFSQNANAH"
     gene            583..1257
                     /locus_tag="N7C74_001471"
     CDS             583..1257
                     /locus_tag="N7C74_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HGU2431369.1"
                     /translation="MRNFIFLMAFFCSSVFATQIPVPESPKYVNDLTGTLTNSEVNTL
                     TNQIKALTQKNYAQLVVLVVETTGDETIEQYATRVFDSWKPGDKDRDDGVLLLVAWQD
                     HTVRIEIGYGLEGIITDAQSGKIIRNSIIPAFKKGDLAGGLQKGISDIESRLTGNNLA
                     TITPTDHPLPFSGWWALLVWAIVLTFISARGYIKTLGVICFAAIVLAFVLPIAGFSGS
                     WGVLAT"
     gene            1491..2639
                     /locus_tag="N7C74_001472"
     CDS             1491..2639
                     /locus_tag="N7C74_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HGU2431370.1"
                     /translation="MRIFLLLLMLCGFQVFAERLPSVTDLTGTLTTEEQTALTQQLQT
                     LEQQNHFQVAVLVTPTTGGKNIKLAASQRYGVYHWKPVGEKRYGEGILILVVWPEGLA
                     SMKIGHGLEEMLPPEQAAQIVRYHMQPEFEKNNLFAGLTGGIESIAQFTHIKANLSPL
                     DALANHLFANPQRSLPCLAWTLLMIVAIVVLWLFASRPRRGIWMISMITPMVWFFSFQ
                     DDVIIRRVSVVCFSLLLATTCKQRLAVVFNMCRVFPMMLTPKNKNAKVKKQKKTPQVR
                     ESGRNSLFVVMLIIIVGWCLVFASNKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAK
                     FTGNLKLGSGEKSNRKTGNRNAHSRSSSRNSFRGGGGSSGGGGSSVRW"
     gene            2636..3550
                     /locus_tag="N7C74_001473"
     CDS             2636..3550
                     /locus_tag="N7C74_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311665.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HGU2431371.1"
                     /translation="MIVMRLIFILFTLWCLPGLAQQIAVPELRQQVTDITGTLSTSEQ
                     QSLTQQLQDITHKTRAQVAVLVVPSTGDDSIEQYATRVFDSWKLGDKQRNDGILLLVA
                     WEDHAVRIEVGYGLEGVVTDLQAAKIIRDILIPAFKSDDLMGGLTLASENIGALLLNG
                     ELPEDRGDYYSINPPIPLSLAVIILLAVLSYFIVFTDPSNLPWITLTGAIYGMVFLYV
                     AEPGPWTNLIVACGMLTPFAIVPLVIFWLIVNKKLRAKYKKLSKDRASRKGSSSSSSG
                     GGSSGGGSGGGFSGGGGSSGGGGASGRW"
     gene            complement(3610..4908)
                     /gene="eno"
                     /locus_tag="N7C74_001474"
     CDS             complement(3610..4908)
                     /gene="eno"
                     /locus_tag="N7C74_001474"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HGU2431372.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(4996..6633)
                     /gene="pyrG"
                     /locus_tag="N7C74_001475"
     CDS             complement(4996..6633)
                     /gene="pyrG"
                     /locus_tag="N7C74_001475"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HGU2431373.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(6861..7652)
                     /gene="mazG"
                     /locus_tag="N7C74_001476"
     CDS             complement(6861..7652)
                     /gene="mazG"
                     /locus_tag="N7C74_001476"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HGU2431374.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(7723..8058)
                     /gene="mazF"
                     /locus_tag="N7C74_001477"
     CDS             complement(7723..8058)
                     /gene="mazF"
                     /locus_tag="N7C74_001477"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HGU2431375.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(8058..8306)
                     /gene="mazE"
                     /locus_tag="N7C74_001478"
     CDS             complement(8058..8306)
                     /gene="mazE"
                     /locus_tag="N7C74_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HGU2431376.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(8384..10618)
                     /gene="relA"
                     /locus_tag="N7C74_001479"
     CDS             complement(8384..10618)
                     /gene="relA"
                     /locus_tag="N7C74_001479"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HGU2431377.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(10666..11967)
                     /gene="rlmD"
                     /locus_tag="N7C74_001480"
     CDS             complement(10666..11967)
                     /gene="rlmD"
                     /locus_tag="N7C74_001480"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGU2431378.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLEVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNCLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            12024..14780
                     /gene="barA"
                     /locus_tag="N7C74_001481"
     CDS             12024..14780
                     /gene="barA"
                     /locus_tag="N7C74_001481"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HGU2431379.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(15013..16353)
                     /gene="gudD"
                     /locus_tag="N7C74_001482"
     CDS             complement(15013..16353)
                     /gene="gudD"
                     /locus_tag="N7C74_001482"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HGU2431380.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(16374..17714)
                     /gene="gudX"
                     /locus_tag="N7C74_001483"
                     /pseudo
     CDS             complement(16374..17714)
                     /gene="gudX"
                     /locus_tag="N7C74_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
     gene            complement(17716..19068)
                     /gene="gudP"
                     /locus_tag="N7C74_001484"
     CDS             complement(17716..19068)
                     /gene="gudP"
                     /locus_tag="N7C74_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HGU2431381.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIAISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVVGQ
                     "
     gene            complement(19503..19952)
                     /gene="yqcA"
                     /locus_tag="N7C74_001485"
     CDS             complement(19503..19952)
                     /gene="yqcA"
                     /locus_tag="N7C74_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HGU2431382.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(19970..20752)
                     /gene="truC"
                     /locus_tag="N7C74_001486"
     CDS             complement(19970..20752)
                     /gene="truC"
                     /locus_tag="N7C74_001486"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HGU2431383.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGERSS"
     gene            complement(20752..21081)
                     /gene="yqcC"
                     /locus_tag="N7C74_001487"
     CDS             complement(20752..21081)
                     /gene="yqcC"
                     /locus_tag="N7C74_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HGU2431384.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(21703..22248)
                     /gene="syd"
                     /locus_tag="N7C74_001488"
     CDS             complement(21703..22248)
                     /gene="syd"
                     /locus_tag="N7C74_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HGU2431385.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLAPNLAEFLNQLKPLL"
     gene            22316..23164
                     /gene="queF"
                     /locus_tag="N7C74_001489"
     CDS             22316..23164
                     /gene="queF"
                     /locus_tag="N7C74_001489"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311681.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HGU2431386.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFQGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGEVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            23276..24640
                     /gene="ppnN"
                     /locus_tag="N7C74_001490"
     CDS             23276..24640
                     /gene="ppnN"
                     /locus_tag="N7C74_001490"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HGU2431387.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            25197..26486
                     /gene="sdaC"
                     /locus_tag="N7C74_001491"
     CDS             25197..26486
                     /gene="sdaC"
                     /locus_tag="N7C74_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HGU2431388.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            26544..27911
                     /gene="sdaB"
                     /locus_tag="N7C74_001492"
     CDS             26544..27911
                     /gene="sdaB"
                     /locus_tag="N7C74_001492"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HGU2431389.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMCG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            28023..28778
                     /gene="xni"
                     /locus_tag="N7C74_001493"
     CDS             28023..28778
                     /gene="xni"
                     /locus_tag="N7C74_001493"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HGU2431390.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(28833..29981)
                     /gene="fucO"
                     /locus_tag="N7C74_001494"
     CDS             complement(28833..29981)
                     /gene="fucO"
                     /locus_tag="N7C74_001494"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HGU2431391.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(30009..30656)
                     /gene="fucA"
                     /locus_tag="N7C74_001495"
     CDS             complement(30009..30656)
                     /gene="fucA"
                     /locus_tag="N7C74_001495"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HGU2431392.1"
                     /translation="MERNKLARQIIETCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         7549 a         8134 c         7449 g         7788 t
ORIGIN      
        1 tcaatttatc aggctgtgga tataacgata ttcaaaccta tgatgagcag gttaacgcct
       61 cctggtctga ggtattgaat caatatcaac gtcgcgccga tcttattcct aatcttgtcg
      121 ccagcattaa aggttattcc agccatgaac gagatgtact ggaagccgta acattggctc
      181 gtagccaggc taatcgcgcc agtagcgatc ttcagcaaac tccgggagat gaacaaaaat
      241 tacaagcctg gcaacaggct caagcgcagg tgactcgcac tctcggtcag ttaacgataa
      301 ttagtgagcg ttaccctgag ttaaaatctc aggaactcta tcaaaattta atggtacagc
      361 ttgaaggaag tgaaaatcgg attgccgttg cgcgaggaag atatattaaa gccattgaac
      421 aatataatgt cactatccgc aaattccccg ctgtattaac cgcaaaggtc atggattaca
      481 caccaaagaa aaattatttg ccggatgatg tcgccgcagt aagtaaagcc ccaacaatag
      541 attttagcca gaatgctaat gctcattaag cggggaagtt ctatgcgaaa ttttatcttc
      601 cttatggctt tcttctgttc atctgtgttt gccacacaaa ttccagtacc tgaatcaccc
      661 aagtatgtga atgacttaac cggcacatta acaaacagcg aagtaaacac cctgaccaac
      721 cagattaaag ccctgacgca aaaaaactac gcacagttgg tcgtgttagt ggttgaaaca
      781 acaggcgatg aaaccatcga gcagtacgcg acgcgggtat tcgatagctg gaaaccgggt
      841 gataaagacc gcgatgatgg cgttctactg ttagtcgcct ggcaggatca taccgtgcgt
      901 attgaaatcg gttacggact ggaaggtatt attacggatg cgcagtccgg gaaaattatt
      961 cgcaacagta ttattcctgc atttaaaaaa ggcgacctgg ctggtggttt gcaaaaggga
     1021 atcagcgata ttgaaagtcg tctgacgggg aataatttag caacgataac accgaccgat
     1081 caccctttgc cctttagcgg ttggtgggca ttgttagtct gggcgatagt actgacgttt
     1141 atttctgcca gaggatatat caaaacgctg ggagtaattt gcttcgcagc tattgtactg
     1201 gcctttgttt tacctatagc aggcttcagc ggaagctggg gagtactcgc aacatgactc
     1261 tgcttcgcca caccgttcct ggctgtcgcc atcgctttta cccccttcgg caaaaaagtt
     1321 cgggatttga tgcgtgatgc caaccagcca tcacgccata caagaagcaa ttcttcctcc
     1381 cactattcat catcgtcatc ctcttcattc gacaatgaca attttagcgg tggtggcggt
     1441 tcatctggtg gcggtggcgc ttccgggcgc tggtaacaaa aggaattgat atgcgtattt
     1501 ttctcttact tctgatgcta tgcggttttc aggtattcgc tgaacgattg ccttcagtga
     1561 ctgacttaac cgggacatta acgacagaag aacaaactgc tttaactcaa caattacaga
     1621 ctctcgagca gcaaaaccac ttccaggtgg ccgtgttggt tacgccgacc acagggggca
     1681 aaaacattaa acttgcagcc tcgcagagat acggggtata tcactggaaa cccgttggag
     1741 agaagcgata tggcgaaggt atcttaattc tcgtcgtatg gccggaaggg ctggcgtcaa
     1801 tgaagattgg acatggactg gaagagatgt tgccgccgga acaagctgcg caaattgttc
     1861 gttaccatat gcagccagaa ttcgaaaaaa acaatctctt tgctggatta acgggcggaa
     1921 ttgaaagcat cgcacagttt acccatatca aagcgaacct tagccctttg gatgctttag
     1981 ccaatcatct gtttgctaat ccgcaacgtt cgctgccatg tcttgcctgg acactgctga
     2041 tgatcgttgc catcgttgtc ctctggctat ttgccagccg cccacggcgt ggcatatgga
     2101 tgatatcgat gattacccct atggtctggt ttttctcctt tcaggacgat gtcatcattc
     2161 ggcgggtatc ggtcgtttgt tttagtttat tgttagcaac aacctgcaag cagcgtctcg
     2221 cggtagtttt taatatgtgc cgcgtatttc ctatgatgct gaccccaaaa aataaaaacg
     2281 ctaaagtgaa aaaacaaaag aagactccgc aggttcgtga atcaggacga aattcattgt
     2341 ttgtcgtgat gctgataatt attgtgggat ggtgcctggt gttcgccagt aataaagata
     2401 ttgcattcat ctcaacaatt attgggttaa ttgatcattt tatcggccca ataaccattg
     2461 ctggcatcgt tctgctgatc attgtggcca aatttactgg caatcttaag ctggggtcag
     2521 gagaaaaaag caacaggaaa acggggaatc gtaatgctca ttctcgctct tcgtcgagaa
     2581 attctttccg cggtggtggc ggttcttcag gaggcggcgg ttcatccgtc cgttggtgat
     2641 cgtaatgcgc ttaatcttta ttctattcac tttgtggtgc cttcctgggc ttgcccagca
     2701 aattgctgtg ccggaactgc gccaacaggt gaccgatatt actggtacgt taagcacttc
     2761 tgaacaacaa tcactgacac agcaactgca ggatattaca cataaaacac gggcgcaggt
     2821 cgctgtatta gtagtgccgt ccaccggaga cgattccatt gaacaatatg ctacaagggt
     2881 ttttgatagc tggaaactgg gagataagca gagaaacgat ggtattttac ttctggttgc
     2941 ctgggaggat catgctgttc gtattgaggt tggctacggg ctggaagggg tggttaccga
     3001 cctgcaagcg gcgaagatta tcagggatat attgatccct gccttcaaaa gtgatgacct
     3061 gatgggtgga ttaacactgg caagcgaaaa tatcggcgcg cttctgttga atggtgaatt
     3121 accggaagac aggggggatt attacagtat caatccccct attccattat cgcttgctgt
     3181 aattatattg ctggcggtac tttcatattt tattgttttt accgatccgt cgaatttacc
     3241 ctggatcact ctcactggcg ccatttacgg gatggtattc ctctatgttg ccgagccagg
     3301 accatggacc aatttaatcg ttgcctgcgg tatgttaacg ccttttgcga tcgtaccact
     3361 ggttattttt tggcttatcg tcaataaaaa gctacgcgcc aaatacaaaa aactcagtaa
     3421 agatagagct tcaagaaaag gttcttcttc atcctcttcc ggtggcggct caagtggcgg
     3481 aggctcaggc ggcggattta gcggcggggg cggttcttct ggcggtggcg gcgcgtccgg
     3541 tcgctggtaa ctcaaccata aaaaacgcca gcccgaaggc tggcattttt aaatcagata
     3601 aagtcagact tatgcctggc ctttgatctc tttacgaccg ttgtacggtg ctttttcgcc
     3661 cagagcttct tcgatacgaa tcagctggtt gtatttagca acgcggtcag aacggctcat
     3721 agaaccagtt ttgatctggc ctgcagcagt accaacagcc aggtcagcga tggtagcgtc
     3781 ttcagtttcg ccagaacggt gagagataac tgcagtgtag ccagcgtctt tcgccatctt
     3841 gattgcagcc agagtttcgg tcagagaacc gatctggttg aatttgatca ggatggagtt
     3901 agcgatacct ttttcgatac cttctttcag gatcttggtg ttggttacga acaggtcgtc
     3961 accaaccagc tggattttgt cgcccagaac tttggtctgg tatgcgaaac catcccagtc
     4021 agattcgtcc agaccgtctt cgatggaaac gatcgggtac tgtttggtca gttcttccag
     4081 gaagtgagtg aattcttcag aggtgaatgc tttgttgcct tcgccagcca gaacgtattt
     4141 accgtctttg tagaattcag aagctgcgca gtccatcgcc agagtgatgt ctttgcccag
     4201 ttcataacca gcagctttaa cagcttcagc gataacagcc agagcttcag cgttggaacc
     4261 caggttcggc gcatagccac cttcgtcacc aacagcagtg ttcatgcctt tcgctttcag
     4321 aacttttgcc aggtgatgga aaacttcaga acccatgcgg atggcttctt tcacagtttt
     4381 cgcgccaacc ggctgaatca tgaattcctg gatatcaacg ttgttgtcag cgtgctcacc
     4441 accgttgatg atgttcatca tcggaaccgg catggagtat ttgcccggag tgccgttcag
     4501 ttcagcgatg tgctcgtaca gcggcatacc tttagcagct gcagcagctt tggcgttagc
     4561 cagagataca gccaggattg cgttcgcgcc gaatttggat ttgttttcgg tgccgtccag
     4621 gtcgatcatg atcttgtcaa tgccagcctg atctttagca tctttgccaa tcagcgcctg
     4681 agcgatcggg ccgtttaccg cagcaacagc tttggttacg cctttaccca ggaaacggga
     4741 tttgtcgcca tcgcgcagtt ccagagcttc acgggaacca gtagaagcac ctgacggagc
     4801 agctgccata ccgacgaaac caccctccag atgtacttcg gcttcaacag tcgggttacc
     4861 acgggagtcg atgatttcac gaccgatgat ttttacgatt ttggacatta ggttttcctc
     4921 aagtcactag ttaaactgaa actccagaca aacaacgcgt acccagggta cgcgttgccg
     4981 ttctaacttt tttacttact tcgcctgacg tttctggaac tcgctggcgg ctttcacaaa
     5041 gcctgcaaac agcgggtgac catcacgtgg agtagaagta aactccggat ggaactggca
     5101 agccacgaac cacgggtgat tcggaacttc gatgatctcg accaactgat catccccgga
     5161 acggcccgca acgcgcagac ctgcatcttc aatctgtttc aacagcatgt tgttgacttc
     5221 gtaacggtga cgatgacgct caacaattgt cggcgcattg tacagctggc gaaccaggct
     5281 atcgtcaacc aactggcact gctgtgcgcc gagacgcatg gtaccgccga gatcgctctt
     5341 ctcgctacga acttcaacgt tgccgttttc atcgcgccac tcggtaatca gcgccacaac
     5401 cgggtactta cagtctggca caaattccgt agagttggcg ttctccatgt tggcaacatg
     5461 gcgagcgtaa tcaattaacg ccacctgcat acccaggcaa atgcccagat aaggaatatt
     5521 gttctcacgc gcaaaacgcg cggtcgtaat catgccttct acgccacgat agccgaaacc
     5581 gccaggtacg aggattgcat ccagaccttt aaggatttca acgccgcgcg tttcaacatc
     5641 ttgtgaatcg atcagtttga tgttgacgct gacacgattc ttcagcccac cgtgtttcag
     5701 tgcttcaatc acggatttgt aagcgtccgg cagttcaatg tacttgccga ccataccaat
     5761 ggtgacttca cttaccggat tcgcttcttc gaagataacc tgttcccatt cggacagatt
     5821 tgcttccggg cagtttaagc tgaatcgttt acaaatataa tcgtccagcc cctgagattt
     5881 caacaggccc ggaattttat agatggaatc gacgtctttc agagaaataa ccgctttttc
     5941 cggaacatta cagaacaatg caatcttcgc acgttcgttc gccggaacgg cgcgatctga
     6001 acgacaaatc aggatgtcag gctggatacc gatggagagc agctctttta cagagtgctg
     6061 agtcggtttg gttttgactt caccagacgc tgccatgtac ggcaccagcg tcaggtgcat
     6121 aaacagagtg tgctcacggc caatttcaac agccatctgg cgaatcgctt cgaggaacgg
     6181 cagggattcg atatcaccta ctgtaccgcc gatttcgacc agtactacgt catgaccttc
     6241 gccaccttcc agcacgcgct ctttgattgc gttagtgatg tgcggaataa cctgtacggt
     6301 tgcgccgagg tagtcaccgc ggcgttcttt acgcagaacg tcagagtaga tacgacccgt
     6361 ggtgaagttg ttgcggcggc tcattttggt acggatgaaa cgctcgtagt gccccaggtc
     6421 caggtcggtt tcagcgccgt cttcagtaac gaacacttcc ccgtgttgga ttgggctcat
     6481 agtacctgga tcgacgttga tgtacggatc cagtttcatg atggtcacat tgaggccacg
     6541 ggcttcaaga atggctgcga gggaggctgc ggcaatgcct ttacccagag aggatacgac
     6601 cccgccggtc acaaaaatat agttcgttgt catgctgaac ctgagaagtt aggttgaaaa
     6661 gacgatggaa taaccaggac gggaaagcag tatacccgaa catgacctgt gccacaaact
     6721 ttcattatcc ctcctcttcg ccagcgcact attgaaatca ggagtgagaa aatagcccct
     6781 ttggggtaaa tgtttttgac gcaaatcaag cgcttgtcat ttaaaaaatg acacaaatgg
     6841 cgcttgaccg cgtaattccc ttagagatca atttcctgcc gttttacctg ttgccagact
     6901 tcttccattg tttcgaggtc aacacctgtc atttccagtc cacgcgcggc aacaatacgc
     6961 tccacttcgc gaaaacgacg ctcgaatttt tcgttcgctt tttgcaatgc gatttccgct
     7021 tttgtcccta aatgacgagc cagattgacc gtggcaaaca gcaggtcccc catttcctcc
     7081 tccagtttag cctggtcgac aacagcctgc cgcgcttcgt acatcacctc gtcgatctct
     7141 tcgtagactt tatcgactac cggaccaagc gtcgtccaat cgaagccaac gttggcgcaa
     7201 cgtttctgga ttttttgcgc acgcattaaa gccggtaaac tacgaggaat atcgtccagc
     7261 gccgaatgct gcgctttctg cgcgcgctct tcggttttga tttgctccca acgggcaagc
     7321 acttcactac tgttttcggc agaactgtca gcaaaaacat gcggatggcg acgctctaat
     7381 ttatcgctaa tcgcagcgca aatatcatta aagtcaaagc gcccttcttc ctgagccatt
     7441 tgggcgtaaa acaccacctg gaacagcaga tcgccgagtt caccgcgcag atcgtcaaaa
     7501 tcttcacggg cgatggcgtc cagcacttcg taggtttctt caagggtgta aggcgcaatg
     7561 gtggcaaatg tctgctcttt atcccacggg cagccgtttt ccgggtcgcg caggcgctgc
     7621 ataatagtga gcaaacggtc gatttgattc attagaatgt cctgaaaatt gcgggtccgt
     7681 caggtggaaa cctgtgacca gaatagaagt gagttagtaa cactacccaa tcagtacgtt
     7741 aattttggct ttaatgagtt gtaattcctc tggggcaacc gttcctttct tcgttgctcc
     7801 tcttgcccgc caggcgatac tttttacctg atcagctaac gctacgccat cacgttcctg
     7861 accggataaa acaacttcga acggatatcc ttttgattgc gttgtacaag gaacacacag
     7921 acacatacct gttttgttgt tgtacatgaa cggactcagg acaacagccg gacgatgtcc
     7981 ggcttgctcg ctaccttttg tcgggtcaaa atcaacccaa atcagatcgc ccatatcggg
     8041 tacgtatcgg cttaccatta ccagacttcc ttatctttcg gctctcccca gtcgatattc
     8101 tcgtggaggt tttccggcgt gatgtcgttg accagttcag caagcgtaaa tacgggctct
     8161 ttacgcactg gctcaataat taatttgcca tccaccaggt caatcttcac ttcatcatca
     8221 atattcagat tgagcgcctg cattaacgta gccgggatcc gcaccgccgg tgaatttccc
     8281 caacgcttta cgctactgtg gatcataacc ctttcctcaa accactatca tatgtagata
     8341 cagtatatat caatctacat tgtagatacg agcaaatttc ggcctaactc ccgtgcaacc
     8401 gacgcgcgtc gataacatcc ggcacctggt tgagtttacc cagcacgcgc cccagcactt
     8461 gcaggttgta aatctcaatg gtcatgtcga tggtcgccag ttgctgtttg gtgtcgctac
     8521 ggctggcaac gccaagcacg ttcaccttct cgttggcgag aatggtcgtg atatcacgta
     8581 acaacccact acgatcatta gccaccacgc ggaccaccag cgaatatccg gcggagtagc
     8641 tctcacccca taccgcgtca acaatgcgtt ctggcgcatg ggagcgcagt tccgccagtt
     8701 gttcgcaatc ggcgcggtgt actgaaatac cgcgtccctg ggtaatgaag ccgacaatct
     8761 catctccagg aatcggctgg cagcagcgcg cgatgtggtg catcaggtta ccaacacctt
     8821 caaccactac acgaccgtta tctttactgc ggttttgcgg cgtgtagctt ttttgctgaa
     8881 gctgtttcag cgcggcggcg tcctgctctt cggcactcgg cttattaaat tgcgattgca
     8941 ggaagttcac catctgattg agacggatat ccccgccacc aatcgccgcc agcaactcgt
     9001 cgacatcatt gaagttgtaa cgcggcagca gatgtttttc tgcttctttc aggctgatcc
     9061 ccagatgttc cagctcgtcg tcaaggattt gccgcccagc cagaatgttt ttgtcacggt
     9121 cctgtttacg gaaccaggcg tgaattttcg aacgcccacg gctggttgtg acgtaaccga
     9181 ggtttgggtt taaccagtca cggctggggt tcggctgttt ctgggtgata atttcaatct
     9241 ggtcgcccat ttgcagctgg taggtgaacg gcacaatgcg cccgccaatt tttgccccga
     9301 tacagcggtg tccgacatca ctgtggatgt ggtaagcgaa gtccagcggc gttgatcccg
     9361 caggcaaatc aacgacatca cctttaggcg taaagacgta cacccggtcg tcaaagacct
     9421 ggctgcgtac ttcgtcgagc atttcgccgg aatcagccat ctcttcctgc cacgcaatca
     9481 gtttacgcag ccaggcaatc cggtcttcat gtcccgaacg tgcgccgcca gcagccgcgc
     9541 cctctttata tttccagtgc gcagcaacac ccaactctgc atcttcatgc atctgtttgg
     9601 tgcggatttg gatctcaacg gtttttccac ccggccccag aaccacggta tgaatagact
     9661 gataaccgtt tggtttcggg ttagcgacgt aatcgtcaaa ctcatccggc aggtggcgat
     9721 agtgagtgtg cactatcccc agtgcggcat aacaatcctg taaacgctcg gcgacaatac
     9781 gtaccgcacg cacatcaaac agctcatcga aggcgaggtt ctttttctgc attttgcgcc
     9841 agatgctgta gatgtgtttc ggacgaccat acacttcagc tttaacgcct tcagctttca
     9901 tctcagcgcg cagatgaccg acaaactctt cgatatagtg ttcgcggtcg agacggcgtt
     9961 catgcaacag ttttgcgatg cgtttgtatt cggtcgggtg gagataacgg aagcagtaat
    10021 cttccagctc ccatttcagt tgcccaatcc caagacggtt tgccaacggc gcgtagatat
    10081 tggtgcactc ttttgccgcc agtacgcgtt catcttccgg cgcatctttt acttcacgca
    10141 gatgagcaat acgctccgcc agtttgatga ccacgcagcg aaaatcatcg accatcgcca
    10201 ataacatccg gcgaacgtta tcgacctgtt cggaggaaac agaatcagtg tgcgtcgctt
    10261 tcagctggcg gatcgccgcc atatcacgca cgccgtgaat aaggttaacg accgacttac
    10321 cgacgctctc acgcagcaca tcttcgctga ctacgttggc atcagccaga gggaacagca
    10381 gcgccgcccg cagcgtgtca atgtccatac ttaatgtcga gaggatctcc accatctcaa
    10441 caccacgcca caataacaga ctggcatccg gatgcccctg cgtctgttgc agacaatacg
    10501 cccaggtttc ggctaagcac tcacacgact tctggctggt aatacccaga cttgcgatcc
    10561 atttttccgg atcaaattca ccagccttat tgatatgtgc acttcttacc gcaaccatcg
    10621 tcctctcctt tagggaccag acctgccgaa atcggcaaat cgcaactatt taacgcgcga
    10681 gaaaagtacc atcgattcca gatgtcccgt gtgtgggaac atatccagca tcgccagtcg
    10741 cgcaatggta tatcctgctt ttaataacgc ttcgctatcc cgagccagcg ttgcagggtt
    10801 acaggataca taaactatac gaataggttc cagttttata atttgctgca taacacctgc
    10861 ggcacctgct cgcgccgggt ccagcaacac tttatcgaag ccgtttttcg cccacggctg
    10921 ctttgtgaca tcttcttcaa gattttcgtg ataaaacgtc acattctgta agcagttaag
    10981 acgcgcattc tgctggcctt tttccaccag cgccggaaca ccttctacac ccaccacact
    11041 ggcagcttgt gtcgccaatg gtagtgtaaa gttgcccata ccgcagaaca gatccagtac
    11101 gcgatcttca ggttccacct ccagccattc caacgcacgc gctaccattt tttggttcac
    11161 acccgcatta acctgaataa aatcgcgcgg gctaaaagtt aagcgcaacc cgtttgagtc
    11221 ataccagggc atctcaccag agacggtttc gagtatctca ctatcggggg cgagatacag
    11281 atccaggcct tcagaatgcg aaaagcgttc cagtttttcg cgatctgccg aacttagcgg
    11341 tgcggtatgg cgcaaaatca tcagcgtgcc gctggttgcc tgtaccagtt caacatgacc
    11401 aagatggcgc atagcttgta agctgcccag gcatgccctg actttgggca gcaatgcttc
    11461 aagttggggc actaaaatgg gacattgttt aacgtcgaca atgtcactgg agcccgcttt
    11521 gcgaaacccc atctgaagtt gctgtgtttt cggtaagtag ttcagactta aacgcgcgcg
    11581 acggcgatag ccccagggaa catcggcgat cacttcagag acttcgtgtt tcattaatcg
    11641 ggcgagtgcc gcacttttgc ttcgctgctg taaatccacg ctggcgtgtt gttgctgaca
    11701 gccgccgcat acgccaaaat gaggacagcg tggcgtttcg cgttccgggc tatcgcttaa
    11761 ccggcgtacg actttagcgc gggcatactg ttttttatcc tcagtaacag taacttccgc
    11821 gttttcctgc ggcaataatc cggggataaa tagcgttttg ccgttatgtc gcgccacgcc
    11881 ctgaccaaaa gagtcgaggt cgttgactga aacggttatg atctgacgcg tcgtcgtgcg
    11941 tcgttttgca gagtagaatt gcgccattgg cgagactttc tcaatttaac agtgtgacct
    12001 taattgtccc ataacggaac tccatgacca actacagcct gcgcgcacgc atgatgattc
    12061 tgatcctggc accgaccgtc cttattggtt tattgctgag tatctttttc gtcgtgcatc
    12121 gctataacga cttgcagcgt caactggaag atgccggtgc cagcattatt gagccgcttg
    12181 cagtttctac tgaatatggc atgagcctgc aaaatcgcga atctatcggt cagttaataa
    12241 gcgtactgca tcgtcgccat tccgatattg ttcgcgcgat ttcggtttat gatgaaaata
    12301 accgactctt tgtcacctcc aattttcatc ttgatccctc atcaatgcag ctcggcagca
    12361 acgtgccgtt tcctcgccag ctcactgtca ctcgtgacgg cgatattatg atcctccgca
    12421 cgccgattat ttctgagagt tactcccccg acgaatcgcc cagtagcgat gccaaaaata
    12481 gtcaaaatat gttgggatat attgcgcttg agctggatct taaatcggtt cgcttgcagc
    12541 aatataaaga gatctttatt tccagcgtga tgatgctgtt ttgtatcggt attgcgctta
    12601 tttttggctg gcgcttaatg cgcgatgtaa ccggtccgat tcgcaacatg gtgaatactg
    12661 tcgaccgcat ccgtcgcggg caactcgaca gccgagtgga aggatttatg ctcggcgagc
    12721 tggatatgct gaaaaacggt atcaactcga tggcaatgtc gctggctgct tatcacgaag
    12781 agatgcagca caatatcgac caggcgacgt ccgatctgcg tgaaacgctg gagcagatgg
    12841 aaattcagaa cgttgagttg gatctggcga aaaagcgcgc ccaggaagcg gcgcgtatta
    12901 aatccgagtt tctggcaaat atgtcacacg agctgcgtac accactgaat ggtgttattg
    12961 gttttacccg cctgacgctg aaaacagaat taacaccaac gcagcgcgat cacctgaata
    13021 cgattgaacg ttcggcaaat aatttgctgg caattattaa tgatgttctc gacttctcga
    13081 aactggaagc aggtaagctg attctggaaa gtattccatt cccactacgc agcacgctgg
    13141 atgaagtcgt tactctgctg gcacattctt ctcacgataa agggttggaa ctgacgctca
    13201 atattaaaag cgacgtgcct gataacgtga tcggcgaccc actgcgatta cagcaaatca
    13261 tcactaacct ggtggggaat gcaattaaat tcaccgagaa tggcaacatt gatattctgg
    13321 tagaaaaacg tgcgctgagt aataccaaag tgcagattga agtgcagatt cgggataccg
    13381 gcattggtat tcctgaacgc gatcaatcgc gcttattcca ggccttccga caggctgatg
    13441 ccagtatttc ccgccgtcat ggtggcaccg ggctggggct ggtgattaca caaaaactgg
    13501 ttaatgaaat gggcggcgat atttcgttcc atagccagcc gaatcgcggt tcaactttct
    13561 ggttccacat taatctcgat ctgaacccga acattattat tgaagggcca tccacccagt
    13621 gcctcgccgg taaacgcctg gcctatgtcg aaccaaactc cgcagcagcg caatgcacgc
    13681 tggatatttt aagtgaaacg ccgctggaag tggtttatag cccaacgttc tccgcgctgc
    13741 ctcccgcgca ttacgacatg atgttgttag gcatcgcggt gaccttccgc gagccgctaa
    13801 caatgcaaca tgagcgatta gcgaaagcgg tatcgatgac cgatttcctg atgctggcac
    13861 ttccttgcca tgcacaagtc aatgctgaaa aactcaagca agatggtatc ggcgcgtgtc
    13921 tgctgaaacc attaacacct acgcgcctgt tgcctgccct gacggaattt tgtcatcaca
    13981 aacaaaacac gcttttgcct gtaaccgatg aaagtaagct ggcaatgaca gtcatggcgg
    14041 ttgatgacaa ccccgctaac ctgaaactta tcggcgcatt gctggaagat atggtgcaac
    14101 atgtggaact ttgcgatagc gggcatcagg cggttgaacg ggcgaaacag atgccgttcg
    14161 atttgatctt aatggatatt caaatgcctg acatggatgg cattcgggcc tgcgagctca
    14221 tccaccagct cccccatcag caacaaacgc cggttatcgc ggtaacggcg catgcaatgg
    14281 ccgggcaaaa agagaagctg cttggcgcag ggatgagcga ttatctggcg aaaccgattg
    14341 aagaagagcg attgcataat ttgttgttgc gctacaagcc tggcagcggt atttcgtctc
    14401 gcgtcgtgac gcccgaagtc aacgaaattg tggtgaaccc gaatgcgacc ctcgactggc
    14461 aactggcact acgccaggca gcaggaaaaa ccgatttagc gcgcgatatg ctgcaaatgt
    14521 tactcgattt cctgcctgaa gttcgcaaca aagttgagga acagctggtt ggagaaaacc
    14581 cggaaggcct ggttgatttg attcataaac tgcatggcag ttgcggctat agcggtgtgc
    14641 cgcgtatgaa gaatctctgc caacttatcg aacaacagct acgtagcggt actaaagaag
    14701 aagatttgga accggagctg ctggaactgt tggacgagat ggataatgtc gcgcgcgaag
    14761 ccagcaaaat tctcgggtaa cggacgtctt gtcgagtagc aatcggcttt ttatgacgct
    14821 gttttcagat tggtgacaaa gtgcctgtgt ttatgccgga tgcggcgtta acgccttatc
    14881 cggcctacaa aaccaattaa attcaataaa ttaaaaagca tgtaggcctg ataagcgtag
    14941 cgcatcaggc aattttgtgt ttggcatcag tctcaaaccg gctccagata gagccggttt
    15001 tggttttttg tcttaacgca ccatgcacgg gcgcttgtta tcgaacgtcc agccaggaat
    15061 cagatactgc attcccatcg catcgtcacg cgcgccaagc ccgtgtttct gatacagctc
    15121 atgggctttc atcacttgat ccatatcgat ttctacaccc agccccggtt tttctggcac
    15181 ctgtaccagc ccgcctttga tctcaaacgg ttctttggtc aggcgctgat taccttcctg
    15241 ccagatccag tgcgtatcaa tagcagtaat tttacccggt gcagcggcgg caacatgggt
    15301 aaacatcgcc agggaaatat cgaagtggtt gttagagtgt gaaccccagg tcaggccaaa
    15361 ttcatggcac atttgcgcca cacgtaccga accttgcatt gtccagaaat gcggatccgc
    15421 cagcgggata tcaacggatt gtaacgagag cgtatggccc atctgccgcc agtcggtggc
    15481 gatcatattg gttgcagtcg gcaggccagt cgcgcgacgg aactctgcca tcacttcacg
    15541 cccggagaaa ccttgctccg caccacacgg atcttctgca taagccagcg aacctttcag
    15601 gtatttgccg attttaatcg cttcgttcag cgaccaggca ccgttaggat cgagcgtaat
    15661 acgcgcctgc gggaagcgtt gcgccagtgc cacaatagac tcggcctctt cttccccggc
    15721 cagtacaccg cctttcagtt tgaaatcgtt gaagccatat ttttcatacg ccgcttccgc
    15781 caggcgcacc accgcatccg gcgtcatcgc ttcttcatga cgcaggcgat accagtcgca
    15841 tgagtcatcc ggctggcttt gatacggcag aggtgtggct ttgcgattac cgacgaagaa
    15901 cagataaccg agcatttcga cttcgctacg ctgttgacca tcgcccagca gggatgccac
    15961 gttaaccccc agatgctgcc ccagcagatc cagcattgcc gcttctatcc cggtaactac
    16021 atgaatagtg gtacgcaaat caaatgtctg caaaccgcgc ccgccggcat cacgatcggc
    16081 aaaagtatta cgcaccagcg tcagaacgtt tttgtattca cccagcgttt tacccactac
    16141 cagcggaatt gcatcttcca gcgttttacg gattttctcg ccgccgggaa tttcccctac
    16201 gccagtgtga ccagaattat ctttgataat cacaatatta cgcgtgaaga acggtgcgtg
    16261 tgcaccactc agattcatca gcatactgtc atggcccgcc accgggatga cctgcatttc
    16321 agtaacaaca ggcgtcgtaa attgagaact catatttatg tcctttttca gaatcaatga
    16381 cggccgaaaa cgggacgttt acggtcaaag gtccagccgg ggatcaggta ctgcatcggg
    16441 cctgcgtcgt tacgcgcacc gccaggcaga cgtttatagg cctcatgtgc cttttgtacc
    16501 tgttcccagt ccagttccac gcccagaccg ggcgcatcag gaacggcaat ttttccgttt
    16561 ttaatctcca gcggattttg ggtcaggcga caatcgccct cctgccaaat ccagtgggta
    16621 tcgatagcgg taggattacc cggtgccgcc gcgcccacat gggtaaacat cgccagagag
    16681 atatcgaaat ggttgttgga atggcagccc caggtcagcc cccagtcgtc gcaaagctgc
    16741 gccactcgga ctgcaccgga aagcgtccag aagtgcggat cggcaagtgg aatatctacc
    16801 gcattgagca tcaccgcatg acccatttcg cgccagttgg tggcgatcat attagtcgcg
    16861 acgggcaagc cggtcgcccg ccgaaactcc gccatcactt cacgtccgga gaagccctgt
    16921 tctgcgccgc atggatcttc ggcataggta agaacatcat tcagcccttt gcacagagaa
    16981 atggcttcat caagcagcca tgcaccgttg ggatcaacgg taatccgcgc atccgggaag
    17041 cgtttcttca atgcacgaac agtgtcgatt tcttgctcgc caggtaacac gccgccctta
    17101 agtttgaaat ctttaaagcc gtagcgatcc tgtgaggctt ccgccagacg cacaacggct
    17161 tcgctgttca tcgctttctg atggcgcaac tgataccact catggttgcc cggcgtattt
    17221 tccagataag gaagatcggt tttggtccga tcaccgatat aaaacagata accgaggacg
    17281 gtaatagcat cgcgttgctt gcctggccct aacagttcgc agaccggaac attcagcgcc
    17341 ttacctagca ggtcaagcaa ggcggcttcc agcgccgcca cggcgttaac gcgcaattca
    17401 aaggtccagg cccctttacc gaaggtatca aaatcggctg cttgattacc tttatgcacc
    17461 tgctgaacca ctttattcag gcgcgcaact tcctagccca gcaccatcgg aatagcatcg
    17521 accaatgtct gataaatcac ctctccgcct ggcgcttcac caatgccggt atgcccggcg
    17581 ttatcggtga gtaccacaat attgcgagtg aaatatgcgt tatgcgcccc accaatatta
    17641 agcaacatgc tgtcgtaccc ggcgaccgga atgactttca tatcagtaat aacaggactg
    17701 gattgtgtcg tcatcttatt gccccacgac aggtttcagc tcgatacgct tgatatcgcc
    17761 caccagcacc aggtagctca gtaccgcgat taaggcatga acaccaacat aaatcagcgc
    17821 cccattaaac gagccagtcg tgccaacgat ataaccaatt gcgattggcg tgacgatgcc
    17881 agaaatgtta ccgaacatgt tgaacaggcc accggaaaga ccgctgatct cttttggcgc
    17941 ggtatctgcc attactgccc aacccagcgc cccaatgccc ttaccgaaga aggccagcgc
    18001 cataaagccg atgatcatcc actcaacgtt gacgtagttg cagaacacca tcaccatcga
    18061 cagcaacatg cccattacga tcggtgtttt acgcgcaatg ttcagcgatc ccgtgcggcg
    18121 catcagccag tcggaaataa tcccacccag cacaccaccg ataaaaccac aaaccgccgg
    18181 aacggaagcc acaaagcccg ctttcagaat cgacatccca cgcgcctgca ccagataaac
    18241 cggaaaccag gtaatgaaaa agtaagtcag ggcgttgata cagtactgac cgatataaac
    18301 gccgatcatc atccgtgacc cgagcagttg tttgatctgc ccccacttca cgctgaacgg
    18361 cactttaact ttggtgtttt gctgatccat attgatcagc gcgccacccg cggcgatgta
    18421 ctccagctct ttctgattta cccccggatg ttgatttggc tcgtggatga ctttcaacca
    18481 gataaagctg atggcaatgc ccagaccgcc cataaagaag aagacgtgtg accagcccac
    18541 ttcatgcgtc agccagccca taatcggcgc gaagatcacc gttgcgaagt attgagcgga
    18601 gttaaaaatc gacaccgccg ttcccctttc ctgcgccgga aaccaggccg caacaatgcg
    18661 actgttgccg gggaaagatg gcgcttcagc aagcccgacc aggaagcgca gagtaaacag
    18721 ggcaacgata atgccgaatc cactaaagat atcgacgaag ccttgcagca aggtaaacat
    18781 cgaccagata aagatcgacc agaagtagac gcgttttgaa ccaaaacgat ccagcaacca
    18841 accaccaggg atctgcccga taacataagc ccatgagaag gcagagaaca catagcccat
    18901 tcccacggga tcaaggccga tatctttggc catttccgaa ccggcaatag aaagcgtagc
    18961 gcggtcgccg tagttgaagg atgtgacgat aaacaacatc accactatcc agtaacgagc
    19021 attcgtgcgt ttttccacac tgctcgcagc ctgacttaaa gaactcattg ttgcactcct
    19081 gaaaattcgc gttagccacg ctcactctgg actgcgacat cgccaggaaa tcagaggtga
    19141 cgtagggtgt tttttgccgt ttttataggt cgttcgccga atacggcgcg tgtttatatc
    19201 tggcaatagc agtataaaaa gcgcgccata gcggctcacc gtgcaacaac acaacattaa
    19261 tgcgttcaat gaggcccgat tttggcatta gccctggacg gtggaatcca cttcacggaa
    19321 atgaaaacaa gaacaagaaa ggaagggtta aaacgaagaa ataaagaaga gtatgaaatg
    19381 gatcgcttga ctccaggcaa acgccagtaa aaatccgcgc tatgaagcag tttttactgg
    19441 catttgcctg aaaagattcg attcagcacc gctaaaacga catttaccgc tcgctgaaca
    19501 tatcaggaca acagcgtgcc ccactgttcg acccacggat ttgattccgt ttccggttcc
    19561 gggttctcac tggcgtcaat caacagcatt tcgccaaccc gctgagcgct ctgttcctgc
    19621 agcaaggcat cgaactgttt gccgccattg cagaaattca cataactact gtcgccgagc
    19681 gcaatcacgc cataacgcag attcggctgg aagcccagac tatctttgat tccctgaaag
    19741 agcggcacaa tgctatcagg aaggtcgccc tgcccggtcg tggacgtaac caccagaaca
    19801 tacttatcct gatagggcag ccagtcgctt aattcaggat cttcaaatac cgttgctttg
    19861 tggccctgtg cggtcagaat tgcttctgct tcttccgcca ctaacagcga atttccgtac
    19921 atggtgccga caaaaatacc aatttccgcc ataccgtttt ctccctggat taggaacttc
    19981 tctctccatc ctgacccgat ggcgcactga actcaaccct ttcattttca ggaagcagac
    20041 cgcgccagcc aaattgtgat aacgcctgca tccaggtgtc gtccaggccc gcgtgaatag
    20101 tcagcggctc accagtaaaa ggatgcgtca atgacaactg actggcatgc agcattaacc
    20161 gctgaagacc aaaatgctca gcaccgctgc gattctggcg taaatcgcca tgtttgctat
    20221 cgccaataat cggatgacgc aaatgggcaa gatgtcggcg aagctgatgt ttgcgtccgg
    20281 ttttcggctc cagttccacc aggccgtagc gcgtggtcgg gtaacgtccg gtcgctaccg
    20341 gcatttctac ggtcgccaga ccgcgataat gcgtcactgc tggctgcggg cctttatctt
    20401 cgcgggcaaa tttatcagcg attttgtcca gttcttccac cagtggataa tccagcaccg
    20461 cttcttccat caaccagccg cgcacaatcg catggtaacg tttctggatt tggtgctgtt
    20521 caaactgttg tgccagcagc cgtccggcct cgctggataa tcccatcaac aacacaccag
    20581 aagtgggtcg gtccagacga tgagcagtaa aaacatgctg gcctatctgg tcacgcacgg
    20641 tttgcatgac cactactttc tcgtcgcgat ccagccagct acggtgaacc agccagccgg
    20701 agggtttatt taccgcaacc agccattcat cctgatagag tatttccagc attagctcgc
    20761 atcatccgca aaaagagcat ccagtttttc caactcagcc agaataagcg cgcgttgcgg
    20821 atggtccgtc gccagcgcca tttcataata gggtgcaacg gcaaaagcgc ccggtaacgg
    20881 ctggttgtta tttaacaaat cgtgcattcg cgggatcagc acccactgca accactccag
    20941 tggttccatg gtgtccataa agaacggttg ggtactatta aattgatgcg gctggggttc
    21001 atcgtttcgc cagtgctgat gttcacgcag taacgcttca agcgcctgca actggagacg
    21061 aacgcggtca tgagtggtca taaagcaacc tcaataagaa aaactgccgc gaaggatagc
    21121 aggaataaaa aaagggagca ctgtattcac agtgctcccg gttcgtttcg cagcattcca
    21181 gctacttttg ttgctccctg ctcatccttg acaacttttc ctctggccat gcggccaatc
    21241 gttcatcctg aactattgct tcctgctcac accaccccga tgtgatactt catcctgaag
    21301 tgtcccaggc cttcctgacc caccgaatca tcctgaccgg ttctcattct ccatcctgga
    21361 ggtgtccttt aacgcgtcct gcgtcatcct cttcgcttca tccagaagcc tttccctgaa
    21421 acaccatcct ggtgtgtcct tcagaagtgt catcatcctg atgttcactt cgttgtctga
    21481 ctccctgtcg acgaagatag aatcgtcttt ttcgcgaact cttacaaggc gcttacagac
    21541 aatgccttaa ggaaaatttc atatgaaagt taggaattaa caagcaacat gttgttttta
    21601 aatgaaaata acgattctta tctttttaag tattcgctta tttcctggcg atctcctaca
    21661 gagtctgtaa gagatctctc acaaccagat ggggtatttg gattacagaa gaggcttaag
    21721 ttgattaagg aattccgcaa gatttggggc taaatgcgtc cgtttacgtg ttcccagcgt
    21781 ctctttacac acttcaccgg agagattaca caccgagatg acctctaact cttcttccag
    21841 cgtcgcgata aatagcgtgg gtggcagttt tagccttttc tgagtcacca gatgaccaat
    21901 cagattttcc tgcacccgac ggaagtcgtc ttcactccag gtttgcagca atgtcagttt
    21961 gatatcgcca aactgcgcgt gcatatcccc ggcaaactga gtcgtataaa aggtatgaat
    22021 tgtgggttgt atcacaatat caaaagcgcg ttcaaccgcg tttacatttt gttccccggt
    22081 aaacggctgg ggttgccagt atacggcatc ttcagtagtt gaaataatgc acggtgaagg
    22141 aacgccgtac agttcctcac ttaacggcca gcttttatgc tcttcatgcc acgcatcgca
    22201 gtagcgtgca gtaaaatctt tcagggcttg tgcggtcaaa tcgtccacta atttctctct
    22261 tcacttaaac cagatacact tgtcgtttag tttatctggt ttatgacggt gaaacatgtc
    22321 ttcttatgca aaccatcagg cacttgcggg cctgactctt ggaaaatcaa ccgattaccg
    22381 ggatacctat gacgccagcc tactgcaagg cgttccacgc agcctgaatc gcgacccgct
    22441 gggtctgaaa gcggataacc tgccttttca aggtacggat atctggacgc tgtatgaact
    22501 ttcctggctg aatgcgaaag gtttgccgca ggtcgctgtc ggtcatgttg aacttgatta
    22561 caccagcgta aatctgattg agtcgaagag ttttaagctc tatctcaaca gttttaacca
    22621 gacgcgtttt aataactggg atgaggtgcg ccagacgctg gagcgcgact taagcacttg
    22681 cgctcagggt gaggttagcg tggcgttata tcgtcttgat gaactggaag gccagccgat
    22741 aggtcatttc aatggcactt gcattgatga ccaggatatc actatcgata actatgaatt
    22801 cactactgac tatctggaga atgccaccag tggtgaaaaa gtagtggaag agacgctcgt
    22861 cagccacctg ctgaaatcaa actgcctgat cacccatcaa ccagattggg gttcgatcca
    22921 aattcagtat cgtggacgcc aaattgacag agaaaaactg ctgcgttacc tggtctcatt
    22981 ccgtcatcac aacgagttcc acgaacagtg cgtggaacgc atctttaatg acctgttacg
    23041 cttctgccag ccagagaaat tgagcgttta cgcacgttat acccgtcgtg gcggtctgga
    23101 cattaacccg tggcgcagta atagcgattt tgtcccatcg accacaagac tggttcggca
    23161 ataaattttt tctcaatttt gcgtgctgga ttcacgcaga aggttgtgaa aggtcatcag
    23221 gcagggctat tgtaatcaaa gggaatgacg atattcgtcc cataaggagt ttttcttgat
    23281 tacacatatt agcccgcttg gctccatgga tatgttgtcg cagctggaag tggatatgct
    23341 taaacgcacc gccagcagcg acctctatca actgtttcgc aactgttcac ttgccgtact
    23401 gaactccggt agtttgaccg ataacagcaa agaattgctg tctcgttttg aaaatttcga
    23461 tattaacgtc ttgcgccgtg aacgcggcgt aaagctggaa ctgattaatc ccccggaaga
    23521 ggcttttgtc gatgggcgaa ttattcgcgc tttgcaggcc aacttgttcg cggttctgcg
    23581 agacattctc ttcgtttacg ggcaaatcca taataccgtt cgttttccca acctgaatct
    23641 cgacaactcc gtccacatca ctaacctggt cttttccatc ttgcgtaacg ctcgcgcgct
    23701 gcatgtgggt gaagcgccaa atatggtggt ctgctggggc ggtcactcaa ttaacgaaaa
    23761 tgagtatttg tatgcccgtc gcgtcggaaa ccagctgggc ctgcgtgagc tgaatatctg
    23821 caccggctgt ggtccgggag cgatggaagc gccgatgaaa ggtgctgcgg tcggacacgc
    23881 gcagcagcgt tacaaagaca gtcgttttat tggtatgaca gagccgtcga ttatcgccgc
    23941 tgaaccgcct aacccgctgg tcaacgaatt gatcatcatg ccggatatcg aaaaacgtct
    24001 ggaagcgttt gtccgtatcg ctcacggcat cattatcttc cctggcggtg tgggtacggc
    24061 agaagagttg ctgtatttgc tgggaatttt aatgaacccg gccaacaaag atcaggtttt
    24121 accattgatc ctcaccggcc cgaaagagag cgccgactac ttccgcgtac tggacgagtt
    24181 tgtcgtacat acgctgggcg aaaacgcgcg ccgccattac cgcataatca ttgatgacgc
    24241 cgctgaagtc gcccgtcaga tgaaaaaatc gatgccgctg gtgaaagaaa atcgccgtga
    24301 tacaggcgat gcctacagct ttaactggtc aatgcgcatt gcgccagatt tgcaaatgcc
    24361 atttgagccg tctcacgaga atatggctaa tctgaagctt tacccggatc aacctgttga
    24421 agtgctggct gccgatctgc gccgtgcgtt ctccggtatt gtggcgggta acgtaaaaga
    24481 agtcggtatt cgcgccattg aagagtttgg tccttacaaa atcaacggcg ataaagagat
    24541 tatgcgtcgt atggacgacc tgctacaggg ttttgttgcc cagcatcgta tgaagttgcc
    24601 aggctcagcc tacatccctt gctacgaaat ctgcacgtaa tctcccctcc cccggtgacg
    24661 tttgttgccg ggttttctcg tttttggtca cttactcatc aactcatttc atttgttata
    24721 tgaatgtttc ttaccaccct cacgcgacaa atatcatcac agttaatatg tcatacaatt
    24781 tatattgcaa cgcaaacgtt tccctatttt cataaaccgt attttatcgc tatgaaaaag
    24841 aaattatcgc catgattaac taaaagtatt gattttttca gttcaaccta catatattgc
    24901 gcgccccgga agaagtcaga tgtcgtttaa tgggcaaata ttgcccttaa attctctttt
    24961 acttttgatt tacagagtaa agcgttggga taatctatct tccaagtaga ttattgtatt
    25021 tgggatcaag atcactgata gatacataac ttgtgtgtat ctttccgccc tcaaattatt
    25081 acggcggtaa atgattaagc catcgccgat agacagattt catttttacg gtcaggcacc
    25141 ttcccgggct gaactggcta aaagctgaat tatttgcatt cctccaggag aaatagatgg
    25201 aaacgactca aaccagcacg attgcgtcga aagactctcg tagtgcctgg cgcaagacag
    25261 acaccatgtg gatgctgggc ctttacggca cggcaatcgg cgcgggcgtg ctgttcctgc
    25321 caatcaacgc cggtgttggc ggtatgatcc cgctgatcat catggctatc cttgcgttcc
    25381 cgatgacgtt ttttgctcac cgcggcctga ctcgcttcgt actgtctggt aaaaacccgg
    25441 gcgaagacat taccgaggtt gtagaagaac actttggtat tggcgcaggt aaactgatta
    25501 ccctgctcta cttcttcgct atctacccga tcctgctggt ttatagcgtg gcaatcacca
    25561 atacagttga aagcttcatg tctcaccagc tgggtatgac accaccgccg cgtgcgattc
    25621 tgtcgctgat cctgatcgtg ggtatgatga ccatcgttcg ctttggtgaa cagatgatcg
    25681 ttaaagcgat gagtattctg gtattcccgt ttgttggcgt actgatgctg ctggctctgt
    25741 acctgatccc gcagtggaac ggcgcagcac tggaaacgct gtctctggac actgcatctg
    25801 caaccggaaa cggtctgtgg atgaccctgt ggctggcaat tccggtaatg gtgttctcgt
    25861 tcaaccactc tccgatcatc tcttctttcg ccgttgcgaa gcgtgaagag tacggcgata
    25921 tggcagaaca gaaatgctcg aagatcctgg cattcgcaca catcatgatg gtgctgaccg
    25981 taatgttctt cgtcttcagc tgcgtactga gcctgactcc ggcagacctg gctgcggcta
    26041 aagagcagaa catctcgatt ctgtcttacc tggctaacca ctttaacgca ccggttatcg
    26101 cgtggatggc tccgattatc gcgattatcg ctatcaccaa atccttcctc ggccactacc
    26161 tgggcgcacg tgaaggcttc aacggtatgg tgattaaatc tctgcgtggt aaaggtaagt
    26221 ctatcgaaat caacaagctg aaccgtatca ctgcgctgtt catgctggta acgacctgga
    26281 ttgttgccac cctgaacccg agcatcctgg gtatgattga aaccctgggc ggcccaatca
    26341 tcgcgatgat cctgttcctg atgccgatgt acgcaattca gaaagtaccg gcaatgcgta
    26401 agtacagcgg tcacatcagc aacgtattcg ttgtcgtgat gggtctgatt gcaatctccg
    26461 caatcttcta ctctctgttc agctaagtcc tttcgcgccg ctttcgggcg gcgcttcctc
    26521 cgttttaacg cgatgtattt cctatgatta gcgtattcga tattttcaaa atcggcattg
    26581 gcccttccag ttctcatacc gttggaccaa tgaaagcggg taaacaattt accgacgatc
    26641 tgattgcccg taacctgctt aaagacgtga cccgcgtggt ggttgacgtg tacggctcgc
    26701 tctctctgac tggtaaaggc caccacactg atatcgccat tattatgggc ctggcgggta
    26761 acctgccgga taccgtggat atcgattcca tccctggttt tattcaggat gtgaatactc
    26821 atggtcgcct gatgctggca aacggtcagc atgaagtgga gttcccggtt gatcagtgca
    26881 tgaacttcca tgccgacaac ctttctctgc atgaaaacgg tatgcgcatt accgcgctgg
    26941 cgggtgataa agtcgtttac agtcagactt actactctat tggcggtggc tttatcgttg
    27001 atgaagagca ttttggccag caggatagcg caccggttga agttccttat ccgtacagtt
    27061 cagcagccga tctgcaaaaa cattgtcagg aaaccgggct gtcactctct ggcctgatga
    27121 tgaaaaacga gctggcgctg cacagcaaag aagagctgga acagcacctg gcgaacgtct
    27181 gggaagtgat gtgtggcggt attgagcgcg gtatttccac cgaaggtgtg ttacctggca
    27241 aactgcgtgt tccacgccgt gctgcggcac tacgccggat gctggtcagc caggataaaa
    27301 ccaccactga cccgatggcg gttgttgact ggatcaacat gtttgcactg gcagtgaacg
    27361 aagagaacgc tgctggcggt cgcgtggtga ctgcgccgac taacggtgcg tgcgggatta
    27421 tcccggcagt gctggcgtac tacgataagt ttatccgcga agtgaacgct aactcactgg
    27481 ctcgttacct gctggtagcc agcgccattg gttctcttta taagatgaac gcgtcgattt
    27541 ctggtgccga agtgggttgc cagggtgaag ttggtgtggc gtgctcaatg gcggcggctg
    27601 gtctggcaga actgttaggt gcaagcccgg cgcaggtgtg catcgcggcg gaaatcgcca
    27661 tggagcacaa cctcggtctg acgtgtgacc cggtcgccgg acaggtacag gtgccatgca
    27721 tcgagcgtaa cgccattgcg gcagtaaaag cggtgaacgc cgcacgtatg gcgctgcgcc
    27781 gtaccagcga gccgcgcgtc tgcctcgata aagttatcga aaccatgtac gaaacaggta
    27841 aagatatgaa cgccaagtac cgcgaaacct ctcgcggcgg cctggcaatg aagatcgttg
    27901 cctgcgatta atcgctctcc agaggcctcg ttttgcgagg cctcttcccg atttctcatc
    27961 cagccatagc ctgttccagt atcgaatgtt acccttatcg cctgatcttt aagggggtta
    28021 tcgtggctgt tcatttgctt attgtcgatg cactgaatct tattcgtcgc attcatgccg
    28081 ttcaggggtc gccctgtgtc gaaacctgcc agcatgcgct cgatcagctc attatgcaca
    28141 gccagccaac ccacgcggtc gccgtttttg acgatgaaaa ccgcagtagc ggctggcgtc
    28201 atcagcgttt accagattac aaagcgggtc gaccgccaat gccggaagag ttgcacgacg
    28261 agatgcctgc attacgcgcc gcctttgagc aacgcggcgt cccgtgctgg tcaaccagcg
    28321 gcaacgaagc cgatgactta gccgccacgc tggcggtcaa agtgacacag gccgggcatc
    28381 aggcaacgat tgtttcgaca gataaaggct actgtcagtt actttcaccg acattacgta
    28441 ttcgtgatta cttccagaaa cgttggctgg atgcgccatt tatcgataaa gaatttggcg
    28501 ttcaaccgca gcagttgccc gattactggg gacttgcggg gatcagcagt tcaaaggtac
    28561 cgggtgttgc gggaatcgga ccaaaaagcg ccacgcagct gctggtcgag tttcagagtc
    28621 tggaagggat atatgagaat ctggatgcgg ttgccgaaaa gtggcgcaaa aaattagaaa
    28681 cccataaaga gatggcgttt ctgtgccgcg atattgcccg cttacaaacc gatttgcata
    28741 tcgacggcaa tttacagcaa ttgcggttgg tacggtaacg gcgtgccgga tacgccgcaa
    28801 acgtcgcatc cggcattatc acatcagcgc atttaccagg cggtatggta aagctctaca
    28861 atatcctcaa gcgttgcttc acgcgggttg ccaccggtac aaacatcatc cagtgccgcc
    28921 tgcgccagtg ccggaatgtc ttccttgcgt acaccaacat cacgcaaatg tggcggaata
    28981 ccgacatcac ggttgagagc aaacaccgct tcaacagcgg cattacgcgc ctcttccagg
    29041 ctcatacctt ccactttcac gcccataacg cgcgcgatat cgcggtactt ctcaccggta
    29101 aagtcagcgt tataacgcat gacatgcggt aacaggatgg cgtttgcaac accgtgtgga
    29161 gtgttataaa acgcgcccag tggatgcgcc ataccatgca ccaaccctaa ccccacattc
    29221 gagaagccca tacccgcaac atactgcccg agcgccattt cttctccggc atccttatca
    29281 ccagcaaccg atcctcgcag cgccccagca atgatttcaa tcgctttaat gtgcagtgca
    29341 tcggttagcg cccacgcgcc acgggtaata tacccctcaa tagcatgagt gagcgcatcg
    29401 acacccgtcg cagctttcag cgctggaggc ataccatcca tcatgtcagc gtcaataaac
    29461 gccacctgcg ggatatcatg cggatcaacg caaacaaact tgcgccgttt ttcttcgtca
    29521 gtgatcacgt agttaatggt cacttctgcc gcagtgcctg ctgtggtggg gattgccaga
    29581 atcggtacac tgggtttatt ggtcggggaa agtccttcca ggctacgcac atcggcaaac
    29641 tccgggttgt tgctgataat gccaatcgct ttacaagtat cctgtggaga accaccacca
    29701 atagcgatca ggtaatccgc gccgctattc tggaatacac cgagcccttc tttgacgaca
    29761 gtaattgttg ggttgggcac tacgccgtcg taaatcgccc atgccagccc tgcagcatcc
    29821 atcttatcgg tcactttcgc caccacgccg cattgcacca gcgttttatc ggtgacgatc
    29881 agcgccttct gataaccacg gcgtttcacc tcatcggtta aagccccaac agcaccccga
    29941 ccaaaccatg ccgtttcgtt cagaatcatt ctgttagcca tcatccttct ccttgttgct
    30001 ttacgaaatt actcttcaat ccgtaaccca taggttttga atttctccag cactacggca
    30061 atctcttcat cgctcagcac tggcaccggg tccgtaatcg ccagggtcgt caggtaaagt
    30121 tgcgccagca cttcaacttc atgcgccagc cataacgctt tttccagatt cacctcacaa
    30181 gcgataagcc catgatgttg taacaaagtt gccttacgat ttttgagagc cagcgcaaca
    30241 tgttcagaaa gttcgcgtgt tccaaaggtc gcataaggcg cgcaaggaat agaattaccg
    30301 ccagccgccg caatcatgta gtgaatagcg gggatcggtc ggttaagaat ggaaactgct
    30361 gtgcaatgaa cggcatgatt gtgaacaacc gcgttggcat ccggtctgct ttgataggcc
    30421 gccatatgga aacgccactc gcttgagggg agctttcctt cctcatgttt accgttgcca
    30481 tcaataaaga caatatgcga ctccgtcagt ttttcatatg gaatgcctgt aggcgtaatc
    30541 agcatcccat cctgataacg tacactgacg ttccccgctg tcccctggtt cagtcccagg
    30601 cgggtcattt ccaggcaagt ttcaataatc tgacgagcaa gtttatttcg ttccattagc
    30661 tacctctctc tgattcaaaa cagggcaata atgttgttcc tttcacacta ttgaattagc
    30721 cgtttaatta cccaccatct tcttcctgat taacaagaaa gaaattcaca agcttatatt
    30781 ttgtgaccta gttcaactaa tcacagtaaa taactgcaag ttctcttttt ataaccccat
    30841 taaaaatgac cgctcttaaa aatatttatc aaaacggtca tttttctatt cacccaagcc
    30901 aggaatgccc gttttcggca
//
LOCUS       DBFIFM010000038        30738 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.038, whole genome shotgun sequence.
ACCESSION   DBFIFM010000038 DBFIFM010000000
VERSION     DBFIFM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.038"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            118..1776
                     /gene="ybjD"
                     /locus_tag="N7C74_001496"
     CDS             118..1776
                     /gene="ybjD"
                     /locus_tag="N7C74_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HGU2431393.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQAHHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGHAD"
     gene            complement(1773..2729)
                     /gene="ybjX"
                     /locus_tag="N7C74_001497"
     CDS             complement(1773..2729)
                     /gene="ybjX"
                     /locus_tag="N7C74_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HGU2431394.1"
                     /translation="MNMSQLTERTFTSSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2880..3995
                     /gene="macA"
                     /locus_tag="N7C74_001498"
     CDS             2880..3995
                     /gene="macA"
                     /locus_tag="N7C74_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HGU2431395.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            3992..5938
                     /gene="macB"
                     /locus_tag="N7C74_001499"
     CDS             3992..5938
                     /gene="macB"
                     /locus_tag="N7C74_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HGU2431396.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFMTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6011..6235)
                     /gene="cspD"
                     /locus_tag="N7C74_001500"
     CDS             complement(6011..6235)
                     /gene="cspD"
                     /locus_tag="N7C74_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HGU2431397.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6385..6459
                     /gene="yljB"
                     /locus_tag="N7C74_001501"
     CDS             6385..6459
                     /gene="yljB"
                     /locus_tag="N7C74_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HGU2431398.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6558..6878
                     /gene="clpS"
                     /locus_tag="N7C74_001502"
     CDS             6558..6878
                     /gene="clpS"
                     /locus_tag="N7C74_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HGU2431399.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6909..9185
                     /gene="clpA"
                     /locus_tag="N7C74_001503"
     CDS             6909..9185
                     /gene="clpA"
                     /locus_tag="N7C74_001503"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HGU2431400.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9531..9618)
                     /locus_tag="N7C74_001504"
     tRNA            complement(9531..9618)
                     /locus_tag="N7C74_001504"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9582..9584),aa:Ser,seq:gga)
     gene            complement(9872..10090)
                     /gene="infA"
                     /locus_tag="N7C74_001505"
     CDS             complement(9872..10090)
                     /gene="infA"
                     /locus_tag="N7C74_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HGU2431401.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10375..11079)
                     /gene="aat"
                     /locus_tag="N7C74_001506"
     CDS             complement(10375..11079)
                     /gene="aat"
                     /locus_tag="N7C74_001506"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HGU2431402.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRNYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11121..12842)
                     /gene="cydC"
                     /locus_tag="N7C74_001507"
     CDS             complement(11121..12842)
                     /gene="cydC"
                     /locus_tag="N7C74_001507"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HGU2431403.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDS
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAVIL
                     RRVGLEKQLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(12843..14609)
                     /gene="cydD"
                     /locus_tag="N7C74_001508"
     CDS             complement(12843..14609)
                     /gene="cydD"
                     /locus_tag="N7C74_001508"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HGU2431404.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGETELASTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14732..15697)
                     /gene="trxB"
                     /locus_tag="N7C74_001509"
     CDS             complement(14732..15697)
                     /gene="trxB"
                     /locus_tag="N7C74_001509"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HGU2431405.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16242..16736
                     /gene="lrp"
                     /locus_tag="N7C74_001510"
     CDS             16242..16736
                     /gene="lrp"
                     /locus_tag="N7C74_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HGU2431406.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            16871..20899
                     /gene="ftsK"
                     /locus_tag="N7C74_001511"
     CDS             16871..20899
                     /gene="ftsK"
                     /locus_tag="N7C74_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HGU2431407.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDENIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLSPPPFD"
     gene            21054..21665
                     /gene="lolA"
                     /locus_tag="N7C74_001512"
     CDS             21054..21665
                     /gene="lolA"
                     /locus_tag="N7C74_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HGU2431408.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21676..23019
                     /gene="rarA"
                     /locus_tag="N7C74_001513"
                     /gene_synonym="mgsA"
     CDS             21676..23019
                     /gene="rarA"
                     /locus_tag="N7C74_001513"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HGU2431409.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23110..24402
                     /gene="serS"
                     /locus_tag="N7C74_001514"
     CDS             23110..24402
                     /gene="serS"
                     /locus_tag="N7C74_001514"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HGU2431410.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24641..27085
                     /gene="dmsA"
                     /locus_tag="N7C74_001515"
     CDS             24641..27085
                     /gene="dmsA"
                     /locus_tag="N7C74_001515"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HGU2431411.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSGEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27096..27713
                     /gene="dmsB"
                     /locus_tag="N7C74_001516"
     CDS             27096..27713
                     /gene="dmsB"
                     /locus_tag="N7C74_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HGU2431412.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLMPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27715..28578
                     /gene="dmsC"
                     /locus_tag="N7C74_001517"
     CDS             27715..28578
                     /gene="dmsC"
                     /locus_tag="N7C74_001517"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HGU2431413.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(28614..29240)
                     /gene="ycaC"
                     /locus_tag="N7C74_001518"
     CDS             complement(28614..29240)
                     /gene="ycaC"
                     /locus_tag="N7C74_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HGU2431414.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29555..30703
                     /gene="ycaD"
                     /locus_tag="N7C74_001519"
     CDS             29555..30703
                     /gene="ycaD"
                     /locus_tag="N7C74_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2431415.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7551 a         7582 c         8291 g         7314 t
ORIGIN      
        1 tattcgctca tttttcagac atttgccatg cttaaatgtg atgtcatcac gtattagcaa
       61 ggcctttccc gttatactgc cagcgtaaag gataagtcac atatttctgg aggggatatg
      121 attcttgagc gcgttgaaat tgtgggtttt cgcggtatca accgtttgtc gttgatgctg
      181 gaacaaaaca acgtcctgat tggggagaac gcgtggggta aatccagctt gctggacgcc
      241 ttaaccctgc tgctatcgcc agaatcagat ctctaccatt ttgagcgcga cgatttctgg
      301 ttcccgccgg gagatatcaa cgggcgagaa catcatctgc atattatttt gaccttccgc
      361 gaatcgctgc caggtcgaca tcgggttcgc cgttatcggc cgctggaagc gtgctggacg
      421 ccatgcaccg atggctatca ccgtattttt tatcgtctgg aaggggagag tgcggaagac
      481 ggcagcgtga tgacactgcg cagttttctc gataaagacg gacatccgat tgatgttgag
      541 gatattaacg atcaggcaca ccatctggtg cgtttaatgc cggtgctgcg cttgcgtgat
      601 gcccgtttta tgcgccgtat tcgtaacggc acggtgccaa atgtccccaa tgtggaagtc
      661 accgcgcgcc agctcgattt cctcgcccgt gagttgtcct cacatccgca aaatctctct
      721 gatggacaga ttcgtcaggg actttccgca atggtgcagt tgcttgagca ttatttctct
      781 gagcaggggg ctggacaggc gcgatatcgt ttaatgcggc ggcgggccag caatgagcaa
      841 cgaagctggc gctatctgga catcatcaac cggatgattg accgacctgg agggcgctcg
      901 tatcgggtta ttttgctcgg cctgtttgct actttattgc aggcaaaagg cacattgcga
      961 ctggataaag acgcccgtcc attgttgctg atcgaagatc cagaaacgcg tttacacccc
     1021 attatgcttt cagttgcctg gcatctgttg aatcttttgc ctttacaacg tattgctacc
     1081 accaactctg gcgagctgct ttcgttaact ccagtggaac atgtttgccg cctggtgcgt
     1141 gaatcttctc gtgttgccgc ctggcgtctg gggccaagtg gcttgagcac cgaagacagc
     1201 cgccgcatct cttttcacat tcgttttaat cgtccgtcat cgctgtttgc tcgctgctgg
     1261 ttgctggtgg aaggggaaac ggaaacctgg gttatcaatg aactggcgcg tcagtgcgga
     1321 catcattttg atgccgaagg gatcaaggtc attgagtttg cccagtccgg gctaaagcca
     1381 ctggttaaat ttgcccgccg aatggggatt gaatggcatg tactggtcga tggcgatgaa
     1441 gcagggaaga aatatgccgc tacggtacgc agcctgttga ataatgatcg ggaagccgaa
     1501 cgagaacatt taacggcgtt accggcgctg gatatggaac attttatgta tcgccaggga
     1561 ttttccgatg tgttccaccg cgtggcgcaa atcccggaaa atgtcccgat gaatctgcgc
     1621 aaaattatct cgaaagcgat ccatcgctct tccaaacccg atcttgccat tgaagtggca
     1681 atggaggccg gacgtcgtgg tgtggactcc gtaccgacgc tgctgaaaaa aatgttctca
     1741 cgcgtgctgt ggctggcgcg cggtcacgcg gattaaccgc gaaacatcgt ggccatttgt
     1801 ggctgaatag cgtcgagcat ctcatagcgc cgacggtatt cagcccgttt tttactggcg
     1861 atttcggcaa tctcttttcg tgctatctgt gctggaaggc ggtaatggcg ttcagcatca
     1921 catacgccgc caaccgattc ccagaaagcg ttgtaatcgg catggatctt gccttcttta
     1981 tcgcgataac gcaggctgcg gtaaatatgc gtttcattgc tgacggcaat aatctgctct
     2041 acctgcaaac gttgggcaaa cagacaggcc gcttccatca cgaggcgttt gggaaatagc
     2101 ccgtggcagg ctttcgtcgc attctggatt tcctgatgtg gaatttccca ttttgcgcct
     2161 tgcagtccac caataaacat cgttcttttc ccctgatatt cacacagggt aaacgtgatt
     2221 tctgccagag gaataccttc gctgttgcgg aacaggattg tgctgtcacc ttctttatcc
     2281 attgagatca tcatggtcag ctcaagcgtg aactgctcgc cgtttttgcc ttccagcttc
     2341 gccagttgca gcccgggggt attcaaatat aagctgaatt cttccgccga catacatcca
     2401 cggagtaacg cataatggta acgtaacgcc tccagcaatt gcttacggct aagattcgcc
     2461 gcaaggtaag ggcgatgcag acgcacaggc agtcgcggct ggcgcgttaa caatacattg
     2521 agattaggcc agtgggaaag ttcgttcatc cactcaacgc ttaaacgcgg cataatcaac
     2581 gagcgcagca aaaatttctg gcgaaaacta cggcgatgcc agaatttacc cggccgacac
     2641 tgtccacgtg ccagactaag aaaaagtgac aggctgctga gagattcaga tgacgtaaag
     2701 gtccgttcgg ttagctgcga catattcatg aaatcaatgg ttgtacatga cgtcgatttc
     2761 accattgcgt atcttaacca aacatcaata gtgtgattac taacgtaaat tttagggttt
     2821 tgttgatatt tcgttgaagt taatgacccg gattggcata tggagtattc agaatattta
     2881 tgaaaaagcg gaaaaccgtg aagaagcgtt acgttattgc gctggtgata gtcatcgccg
     2941 gactgattac gttatggaga attcttaacg cacccgtgcc gacttatcag acactgattg
     3001 tgcgccccgg tgatttacag caaagcgtgc tggcgaccgg aaagctggac gcgctgcgta
     3061 aggttgacgt gggcgcgcag gtcagcggtc agttgaaaac gctgtcggtg gcgattggcg
     3121 ataaagtaaa aaaagaccag cttttagggg ttattgatcc tgaacaggct gaaaaccaga
     3181 tcaaggaggt cgaagcaacg ctgatggagc tacgcgcgca gcggcagcag gcggaagcgg
     3241 agctgaaact ggcgcgggtg acgtattccc gtcagcaacg tctggcacaa acgcaggcgg
     3301 tttcactgca ggatctcgac accgccgcga cggagatggc tgtgaaacag gcgcaaattg
     3361 gcaccattga cgcgcaaatc aagcgcaatc aggcttctct cgatacggct aaaaccaatc
     3421 tcgattacac tcgcatcgtt gccccgatgg ccggggaagt cacgcaaatc accactctgc
     3481 aaggccagac ggtgattgcc gcacaacaag caccgaacat tctgacgctg gcagatatga
     3541 gcaccatgct ggtaaaagcg caggtttctg aagcggatgt aatccacctg aagccggggc
     3601 aaaaagcctg gtttacggta cttggcgatc cactgacgcg ctacgagggg caaatcaagg
     3661 atgtactacc gacgccggaa aaggttaacg acgctatttt ctattacgcc cgttttgaag
     3721 tccccaaccc caatggtttg ctgcggctgg atatgactgc gcaagtgcat attcagctca
     3781 ccgatgtgaa aaatgtgctg acgatccctc tgtcggcgtt aggcgatccg gttggcgata
     3841 atcgttataa agtcaaattg ttgcgtaatg gtgaaacacg cgagcgtgaa gtgacgattg
     3901 gcgcacgtaa cgataccgat gttgagattg tcaaagggct ggaagcgggc gatgaagtgg
     3961 tgattggtga ggccaaacca ggagctgcac aatgacgcct ttgctcgaat taaaggatat
     4021 tcgtcgcagc tatcctgccg gtgatgagca ggttgaggtg ctgaagggca tcaccctcga
     4081 tatttatgcc ggtgagatgg tagcgattgt tggcgcttcg ggttccggta aatcgaccct
     4141 gatgaatatt ctcggctgtc tggataaggc caccagcggc acctatcgcg tcgccggtca
     4201 ggatgttgcc acgctggacg ccgatgcgct ggcgcaactg cgccgcgagc atttcggctt
     4261 tattttccag cgttaccatt tgctttcgca tttaaccgcc gagcagaacg ttgaagtacc
     4321 agccgtctat gctggtcttg agcggaaaca gcggctgctt cgcgcccagg agttgctgca
     4381 acgactgggg ctggaagacc gtacagagta ttatccggca cagctttcgg gtggtcagca
     4441 acagcgcgtt agcatcgcgc gggcattgat gaacggcggt caggtaattc ttgccgatga
     4501 accgaccggc gcactggaca gccattcagg cgaagaggtg atggcgatcc tgcatcagct
     4561 gcgcgatcgc gggcatacgg tgattatcgt cactcacgat ccgcaggtcg ctgctcaggc
     4621 cgagcgggtg atcgaaattc gcgacggcga aattgtgcgc aatcctcccg ccattgaaaa
     4681 agtgaatgtt gctggcggga cggaacctgt tgtcaacacg gcgtctggct ggcggcagtt
     4741 tgtcagcggt tttaatgagg cgctgacgat ggcatggcgg gcgctggcag cgaataaaat
     4801 gcgtacttta ctgaccatgc tggggattat aatcggtatt gcgtcggtgg tttccattgt
     4861 cgtggtgggc gacgccgcca aacaaatggt gctggcggat attcgttcta ttggtacgaa
     4921 tactattgat gtctatcccg ggaaagattt tggcgatgac gatccgcaat atcaacaggc
     4981 gctgaagtac gatgacttaa tcgccatcca aaaacaaccg tgggtcgctt cagccacacc
     5041 tgccgtctcg caaaacctgc gcctgcgtta taacaatgtt gatgttgctg ccagtgccaa
     5101 tggcgtgagc ggcgattatt ttaatgtcta tggcatgacc ttcagtgaag gaaacacctt
     5161 taaccaggaa cagctgaacg gtcgtgcgca ggtcgtggtt ctcgacagta atactcgccg
     5221 ccagcttttc ccccataaag cagatgtggt tggcgaggtg attctggtcg gcaatatgcc
     5281 cgccagagtc attggtgtgg cggaagaaaa acagtcgatg tttggtagca gtaaagtgct
     5341 gcgtgtctgg ctaccttaca gcacgatgtc cgggcgagtt atgggccagt cgtggctgaa
     5401 ctccattact gtcagggtga aagaaggatt tgacagcgcc gaggcggaac agcaactcac
     5461 gcgtttactt tcactgcgcc acggtaagaa ggatttcttt acctggaaca tggacggcgt
     5521 cttgaaaacc gttgaaaaga ccacacgtac tttacaactg tttatgacgc tggtggcggt
     5581 gatttcgctg gtagtgggcg gtattggtgt aatgaatatt atgctggtgt cagtgaccga
     5641 gcggacgcgg gaaattggca ttcgcatggc tgtaggtgcg cgagcaagcg atgttttgca
     5701 acagttcctg atcgaagccg tactggtttg cctggtcggt ggcgcgttgg gaataacact
     5761 gtcactgtta attgctttca ccttgcagct tttcttaccc ggctgggaga ttggtttttc
     5821 accgttggca ctgctgctgg cgtttctctg ctcgacggtc accgggattt tatttggctg
     5881 gttacccgca cgaaatgcgg cacgactgga tccagtagat gctctggcac gagagtaatt
     5941 tttgagataa aaatgccagc cgatcgggct ggcattttgc ctttaggatg tacacaatga
     6001 gacagaagag ctatgcgact gccgcttcta cttcgacggg cacaataaca ctggcgtgat
     6061 tgccttttgg cccctggtgg acatcaaact gaacggattg tccagctttt agcgttctgt
     6121 aaccatccat ctgaatggtg gaataatgag cgaaaatatc ttcgccgccg ccttcagggc
     6181 agatgaaacc aaaccctttg gcattgttga accacttaac agtacccttt tccatgcttc
     6241 gacatccttc gcaaatctta tacaagtaag atggaataaa ccggggtcag agagggggct
     6301 gttcaaaacc tcgccaactc tagaaataca atttagagaa ttagggcgag ccgtcaagca
     6361 tttgacaggg gacagggggc aggtatgaat caaaaatttg aagcagttaa cgctattgac
     6421 aggaatgtga cagatgtcgc tgatgccaac gatagatgat agttatctat catgtggagt
     6481 agattggtca ggcaaataag ctcttgtcag cggcagggcg ttctgccgat aaccgtaacc
     6541 gaagatgata actgacaatg ggtaaaacga acgactggct ggactttgat caactggcgg
     6601 aagaaaaagt tcgcgacgcg ctaaaaccgc catctatgta taaagtgata ttagtcaatg
     6661 atgattacac tccgatggag tttgttattg acgtgttaca aaaattcttt tcttatgatg
     6721 tagaacgtgc aacgcaattg atgctcgctg ttcactacca ggggaaggcc atttgcggag
     6781 tctttaccgc cgaggttgca gaaaccaaag tggcgatggt gaacaagtac gcgagggaga
     6841 atgagcatcc attgctgtgt acgctagaaa aagcctgaat gcaggcataa aaattggggg
     6901 aggtgcctat gctcaatcaa gaactggaac tcagtttaaa tatggctttc gccagagcgc
     6961 gcgagcaccg tcatgagttt atgaccgtcg agcacttgtt actggcgctg ctcagtaacc
     7021 catctgcccg ggaggcgctg gaagcgtgtt ctgtggattt ggttgcgctc cgtcaggaac
     7081 tggaagcctt tattgaacaa accacacccg ttctgcctgc cagtgaagag gagcgcgaca
     7141 cacagccgac gctgagtttt cagcgtgtac tgcaacgtgc ggtcttccat gtccagtcct
     7201 ccggtcgcaa tgaggttacc ggtgcaaacg ttctggtcgc tatctttagc gaacaggagt
     7261 cgcaggcggc atatctgttg cgtaaacatg aagtcagccg tctcgatgtg gtgaacttta
     7321 tctctcatgg cacgcgtaaa gacgagccga cacagtcttc tgatcctggc agccagccaa
     7381 acagcgaaga acaagctggt ggggaggaac gtatggagaa tttcacgacg aacctgaatc
     7441 agcttgcgcg cgtgggcgga atcgacccac tgattggtcg tgagaaggag ctggagcgtg
     7501 ctattcaggt tctctgccgt cgccgtaaaa acaacccgct gctagtgggg gaatctggtg
     7561 tcggtaaaac cgcgattgca gaaggtcttg cctggcgaat tgttcagggc gatgtgccgg
     7621 aagtgatggc tgactgtacg atttactctc tcgatatcgg ttctctgtta gcgggcacaa
     7681 aatatcgcgg cgactttgaa aaacgtttta aagcgttgct caagcagctg gagcaggaca
     7741 ctaacagcat cctgtttatt gatgagatcc acaccattat cggtgcgggt gcagcgtctg
     7801 gtggtcaggt tgatgcggct aacctaatca aaccgttgct ctccagcggt aaaattcgcg
     7861 taattggttc gacaacctat caggagttca gcaacatttt cgagaaagac cgtgctctgg
     7921 cgcgtcgctt ccagaaaatt gatattactg aaccgtcgat cgaagaaact gttcaaatca
     7981 tcaatggcct gaaaccgaag tatgaagcac accacgacgt gcgttatacc gcaaaagcgg
     8041 tgcgtgcggc ggtagagctg gcggtgaaat acattaacga tcgtcatctg ccggataaag
     8101 ccattgatgt tatcgacgaa gcgggcgctc gcgcacgtct gatgccggta agcaaacgca
     8161 agaaaaccgt taatgtggcg gatattgagt ccgtggtggc ccgtattgca cgcattccag
     8221 agaagagtgt ttctcagagt gaccgcgata ccctgaaaaa cctcggcgat cgcctgaaaa
     8281 tgctggtctt cggtcaggat aaagccattg aggcgctgac tgaagccatt aagatggcgc
     8341 gtgcaggttt aggtcacgaa cataaaccgg tcggttcgtt cctgtttgcc ggccctaccg
     8401 gggtcgggaa aacagaggtg acggtacagc tttcgaaagc tttgggcatt gagcttctgc
     8461 gctttgatat gtccgagtat atggaacgcc ataccgtcag ccgtcttatt ggtgcgcctc
     8521 cgggatacgt tggttttgat cagggcggtt tgctgactga tgcggtcatc aagcatccac
     8581 atgcggtgct gctgctggac gaaatcgaga aagcgcaccc ggacgtgttc aatattctgt
     8641 tgcaggtgat ggacaacggt acgctgaccg ataacaacgg acgcaaagcg gacttccgta
     8701 acgtggtact ggtgatgacc actaacgccg gggtacggga aactgagcgc aaatccattg
     8761 gtcttatcca ccaggataac agcaccgatg cgatggaaga gatcaagaag atctttacac
     8821 cggaattccg taaccgtctc gacaacatta tctggttcga tcatctgtca actgacgtga
     8881 tccatcaggt ggtggataaa ttcatcgtcg agttgcaggt tcagctggat cagaaaggtg
     8941 tttctctgga agtgagccag gaagcgcgta actggctggc cgagaaaggt tacgaccggg
     9001 caatgggcgc acgtccgatg gcgcgtgtca tccaggacaa cctgaaaaaa ccgctcgcca
     9061 acgaactgct gtttggttcg ctggtggacg gcggtcaggt caccgtcgcg ctggataaag
     9121 agaaaaatga gctgacttac ggattccaga gtgcacaaaa gcacaaggcg gaagcagcgc
     9181 attaatctga ttgtcaggta ggttggtgaa gtccgtaatc tcgaaagagg ttacggactt
     9241 tttatttatg gggtggaacg gtgaaaaccc tatttttgga ggtgaaggta atttgttgat
     9301 aattagtgct gctggaaaag gtaaggataa aaaagggtga cagcaggaaa atgagatggt
     9361 tttgctttat taacaacggg ctaaacgtgt agtatttgag ttcactgccg tacaggcagc
     9421 ttagaaattc actggtaaca tactccaccc gcccaccatg ttcactgccg tacaggcaga
     9481 taaaatgcga aaaaaaagcc cgtactttcg tacgagctct tctttaaata tggcggtgag
     9541 ggggggattc gaacccccga tacgttgccg tatacacact ttccaggcgt gctccttcag
     9601 ccactcggac acctcaccaa attgttttgc tgccaaacct catgggtggc aacggggcgc
     9661 tactataggg agttggagta aaacggtcaa gaagaatttt aatgataatt attgtttgct
     9721 catactgtaa acaacttgtg cagtatatct acatcgagac aagttatgga cttatacttc
     9781 caaagtactt catgcatatc acaaaataaa aaggccggtt aaaccgacct tttactcgtt
     9841 ctttctcttc gcccatcagg cggtaaaaca atcagcgact acggaagaca atgcggcctt
     9901 tgctcaggtc gtacggggtc agttcaacag tcactttgtc gcccgtcagg atgcggatgt
     9961 agtttttgcg cattttaccg gagatgtgtg cagtaaccac gtgaccgttt tctaactcta
    10021 cgcggaacat ggtattaggc aacgtttcaa gaacggtacc ttgcatttca atattgtctt
    10081 ctttggccat ctaatcctct ggggtatcac taccgtaatt tgaaccggca agataatgcc
    10141 gaagttctgt aaataagtaa agatttgcgc gctaaatcgc aacaaacagg ttcggcacat
    10201 tactccgaaa acacacggct aagccgcacc aaaagcgcaa cgtataaggg agcggtgaga
    10261 taaacgatgg gcgttacctg acgcgaaaaa ttccttatcg gcagcggggt aatgagcgta
    10321 accaactctg cgaccgcaat tataacactc tggggagaaa tgtgccgaaa acattcattc
    10381 ttgtggtgaa aacaagcatc gtggtaccca gaaattattc ggcaatcgtc cgaggcgcat
    10441 ttgattgaga taattaaggt aattccggcg gggaatttcg caggcaccaa gcgatgctgt
    10501 gtgatcgtta aggacctggc agtcgataag cttaccgcca tgaccgataa attcctcaca
    10561 gaataccaga agcgccgttt tagacgcatt ttccatccgg ctgaacatgg actcgccaca
    10621 aaatagcgtt ccctgggcca cgccgtacat accgccgaca agctcatctt cacgccagac
    10681 ttcaatggag tgggcatgac cgagttcgtg aaggcgatgg taggcttcga ccacgccacg
    10741 cgtgatccag gttccttctt cgcgatcgct ggcacagcct tcaatgacct gaccaaaagc
    10801 gtaattcatc gtgacacgat agggcgagcg tttatgaaat cgcttcatac tacggctgat
    10861 atgcagtgat tctggccata gcaccgcgcg gggatcgggc gaccaccaga ggatggggtc
    10921 gcctggagaa aaccacggaa aaataccacg ctggtaagcc attaacaggc gcgcagggct
    10981 aagatcgccc ccaagtgcca gcaggccgtt aggctcacgt aatgcgcctt ccggggaagg
    11041 gaaggctatt gaatggcgag aaagctgaac caggcgcatg accgcaaaac tccacgcaag
    11101 tcggatcgtt caataatagc ttacaaaccc tgcttgaact ggtaataacg cccctgtctg
    11161 gcaagcagtt ctgcgtgagt accttgctca ataatttgcc cgttgtccat cactattatt
    11221 tgttggaaac gagagagtcc gcgaagtcga tgggtgacca ttaacaccgt tttctcacgc
    11281 atcatttctg caagcaattc aaggatctgg ctttcggttg tggcatctaa gccttcggta
    11341 ggttcatcca gcaacaccag tggcgcatca tgtaacagcg cacgggcgat agccagacgg
    11401 cgcagttcac caccggagag ctggcgtccg ccttcaccta accaactgtt gagacctgca
    11461 tcctcgagct gcttctccag accaacgcga cgtaagatca ccgccagagc ctcatcacta
    11521 ctgccaggcg aggcgagtaa cagattatca cgcagcgtgg cgctaaacag atgcactcgc
    11581 tgaggaacaa cgctgatggt ctgtcgtaga gccgcttcat tcaggctggc tatggggcta
    11641 tcgttaagca aaatctcgcc ctgttgcggg tcccatgcgc gggtcagcag ttgtaacagt
    11701 gttgatttgc cgcatccggt tcgcccgaga atcgctatat gttccccggc gtttacctga
    11761 agagaaatcc ctttaagtgc ctgttgggat tgctccggat aagtgaactg aacatcccgt
    11821 aatgtcagcg aaacgcgatc ggcaacacga gtttgggtat cagggaaggt gacctccggt
    11881 ttttgatccg ttaagtcaga gatacgtacg gcagaggcaa tgacttgccc cagatgctga
    11941 aatgcacccg ttactggtgc cagtgcttca aacgcggcta acgcgcaaaa gacaaacagg
    12001 gcaattaacg cgccgggttg agcattgccg ccaacgccgc cagacgccat ccacagcatc
    12061 aggatcaccg ctaacgcgcc aatgagcagc attatcgctt gcgacaatgc ggtcagttca
    12121 gattgacggc gttgcgcttc cagccattga atttctgtat tctctagttg cgtgcgataa
    12181 cgatcgctgg caccaaaaat ggtcagctca gcttgccctt gcagccaggc cgtcagttgt
    12241 tggcgatact gtccgcgaag atgagtcaga ttttgcccgg tgctttttcc cgcacgataa
    12301 aacagcggtg gcatcaggaa aagcgtcagt aacataatgc cgcccagcgt aaaggcgagg
    12361 gtggaatcaa ggaaacttaa cccgattgtc accaccataa tcaccacaaa agcgcccacc
    12421 agcggcgaga taacgcgcag gtaaagatga tcgagcgtat caacatccgc caccacgcgg
    12481 ttgagcaatt cgccctgacg atagcgcgcc agtccggcag gggagagggg caacaatttg
    12541 ctgaaggtgt aaatgcgcag atgctgcaac acgcggaaag tcgcgtcgtg acttaccaga
    12601 cgttcaaaat aacgcccggc agtacgggtg attgctgcac cacgcacgcc cgcagcgggt
    12661 agcatatagt tgaagctgta cagtccggca acccccgcaa ccgctgaggc cgagaggaac
    12721 cagccggaaa gtgtcaacag accgatactg gcgagcagcg tcacaattgc cagcacaata
    12781 ccaagactta acatccattt atgacgttta tacagtgcca gatagggtag caaagcgcgc
    12841 atttaaatct cctcctgacg atgggccagt aatgtggcga atgggccacc agccacactt
    12901 aattccgcgt aacgtccttg ctcaataatc cggccatcct gcataaccca aatgacatcc
    12961 cagtcagcaa gatcttctaa ctggtgggtg accattaacg ttgtctggcg cagagaggcg
    13021 gcattcagcg cctccattac gcgctgttca ctgtgagcat caaggctggc agcgggttca
    13081 tccaacagta atagcgaaca gggatttagt aacgcacggg ccaccgccac gcgctgcgcc
    13141 tgccccacgg aaaggcgggc agcctggtcg ccaacaggcg tatcaacgcc ttgtggcagg
    13201 agcggtagaa actcgctgac ccaggcgtta tccagcgctg cttgtaattc ttgttcgctg
    13261 gcatcaggtc gcgccagtag tacgttatcc cgtaatgttg ctgccggtaa ttgtgggttt
    13321 tgaccaaccc aggagagatg tttacgccat gattctggtg ataaatcgcg taattctatc
    13381 ccgttgattc gtaatgatcc ctgatatgag agaaaaccag aaagcgcgtt cagcagtgag
    13441 cttttacctg aaccgctgcg accaaccaac actgcacgtt ggcctgctgg caaagtaaag
    13501 ttcagcggtc cggccagcgt tttaccttcc ggcgacgtga taaacagatc ctcggcctca
    13561 atggtcagcg gatcggttga tgctaattcc gtctcaccgc gctgcggatg ggcgagcggg
    13621 gtttccataa acgttttcag actgtcagct gcaccaacag cctgggcttt agcatgataa
    13681 aacgtaccga gatcgcgtaa tggctggaaa aactctggcg caaggatcag ggccagaaaa
    13741 cccgcagcca gcgtcacgcc agtatcgtag tgaccaaaat ccagctcgcc gagataggaa
    13801 aaaccaaagt agaccgccac cagagcaatt gacagcgagg taaaaaattc gagaatgccg
    13861 gaggataaaa acgccagccg tagcacttcc attgtccgtt ggcggaaatc ttccgaagca
    13921 gaacgaatac tttcaatttc agcttcacca cgaccaaaaa tacgcaatgt ttccatgccg
    13981 cgcaggcgat cgaggaaatg cccacttaag cgagcaagag cgagaaagtt acgtcggtta
    14041 gcatcggcag cccccatccc aaccagcgcc ataaacaacg gaattaacgg tgcagtgccc
    14101 agcagaatga gcgccgcagc ccagttagag gggaagatcg ccaccacaat cagcaacggc
    14161 accgacactg ccagcgccat ttgcggcagg tagcgtgcat agtaatcatg catatcgtca
    14221 atttgctcga gtaccagcgt cgcccagctc cccgcaggtt taccctgaat ccacgctggc
    14281 cctgcttgtt gcagacggtc gagaacctga cggcggatgg caaagcggat atgctgcccg
    14341 gcgtgataac ccacccgttc gcgtaaccag accacccatg cgcgcagtac aaaggtcaga
    14401 accagtaacg taaagggaag cagcagggct tcacggggaa tattctccat aatcatatgt
    14461 tgcagaattc gcgccatgaa ccatgcctgg gcaatgatca atatgccgct cacaaagccc
    14521 agcagacgag aaatattcag ccaacgttgg gagatgacgc tttgctgttt taaccagcgg
    14581 gttaactctt tttgacgaga tttattcatt gcacgcttag caggtgagtt atcagaatta
    14641 tttgcagagc aatgttacaa cggggaaaaa ataaaggcga cccatagtcg catggtgtcg
    14701 ccttctttac ttttgttact gatttgtaaa attattttgc gtcagctaaa ccatcgaggt
    14761 agcgttccgc atcaagtgct gccatgcagc ctgtaccagc agatgtaatg gcctggcgat
    14821 aaatgtgatc catcacgtcg cctgcggcaa agacgccagg aatgctggtc tgggtggcat
    14881 taccatgaat acccgactgc actttgatgt agccgttttc cagttccagc tgcccttcga
    14941 aaatggcagt attcgggctg tgaccgatag caacaaacag accggcaacg tcgagtgact
    15001 cgatgttatc gctgttttgc gtatcgcgca gacgaacgcc agtgacaccc atttgatcgc
    15061 cggtcacttc ttccagcgta cggttggtgt gcagaatgat gttgccgttc tccactttat
    15121 ccatcagacg cttaatgagg attttttccg cgcggaaacc gtcacggcgg tgaatcagat
    15181 gcacttccga agcgatgtta gacagataca gcgcctcttc aaccgcggta ttgccgccgc
    15241 cgatgaccgc aactttctgg ttgcgataga agaaaccgtc gcaggttgca caagcagaaa
    15301 ctccacggcc tttaaaggct tcttcagagg gcaggccgag atagcgtgca gaagctccgg
    15361 tggcaataat cagcgcgtcg caagtgtatt cgccgttatc gccattcaga cggaacggac
    15421 ggttttgcag atccaccttg ttgatatgat caaaaatgat ctcagtttca aacttggtgg
    15481 catgttcgtg catgcgctcc attaataacg gaccggtcag atcgtttgga tcgccaggcc
    15541 agttttccac ttccgtggtg gtggtcagtt ggccgccttt ttccatgccg gtaatcagca
    15601 caggttgcag gttggcgcgc gccgcataga cagcagcggt gtatcccgcc gggcctgaac
    15661 ccaggataag cagtttactg tgtttggtcg tgcccatgag atccccatag ttgttggcag
    15721 acaatgggca ggattgtagg gaatttacag acgtaaaaaa agagtatgac gattttgtta
    15781 acaatttgtg caatcggcag catcgataag caggtcaaat tctcccgtca ttatcacctc
    15841 tgctacttaa atttcccgct ttataagccg attaaatgat gaataaacgc ccctgttaat
    15901 gaatatctgg catgttgtac taaaaatcga tgttttgctt tgacaatccc ctggtgtttt
    15961 gcgaaaacat tcgaggaaga aaaaaaacag tattcttata tgcgcataac catgcatgta
    16021 aataccatgt ttaccgtgct agtgaaatct acgtatggcg tggacagacg ccattcgtga
    16081 tgtcgatagc tgccacaagg caacggtctt ctcaccgtag acccaggcat tgcgcgccgt
    16141 gaatcttcat gatttcggtc tatcgtgacg ggtagcgact ctgaacagtg atgtttcagg
    16201 gtcagacagg agtagggaag gaatacagag agacaataat aatggtagat agcaagaagc
    16261 gccctggcaa agatctcgac cgtatcgatc gtaacattct taatgagttg caaaaggatg
    16321 ggcgtatttc taacgtcgag ctttctaaac gtgtgggact ttccccaacg ccgtgccttg
    16381 agcgtgtgcg tcggctggaa agacaagggt ttattcaggg ctatacggcg ctgcttaacc
    16441 cccattatct ggatgcatca cttctggtat tcgttgagat tactctgaat cgtggcgcac
    16501 cggatgtgtt tgaacaattc aataccgctg tacaaaaact tgaagaaatt caggagtgtc
    16561 atttagtatc tggtgatttc gactacctgt tgaaaacacg cgtgccggat atgtcagcct
    16621 accgtaagtt gctgggggaa accctgctgc gtctgcctgg cgtcaatgac acacggacat
    16681 acgttgttat ggaagaagtc aagcagagta atcgtctggt tattaagacg cgctaacacg
    16741 gaacaggtgc aaaatcggcg tattttgatt acactcctgt taatccatac agcaacagta
    16801 ctggggtaac ctggtactgt tgtccgtttt agcatcgggc aggaaaagcc tgtaacctgg
    16861 agagcctttc ttgagccagg aatacactga agacaaagaa gtcacattga caaagttaag
    16921 tagcggccgc cgccttctgg aggcgttgct gatccttatt gtcctgtttg ccgtctggtt
    16981 gatggctgcc ttactaagct ttaacccttc ggaccccagc tggtcgcaaa cggcctggca
    17041 tgaacctatc cataatttag gtgggatgcc cggtgcgtgg ttggcagata cgctgttctt
    17101 tatttttggc gtgatggctt acaccattcc cgtcattatt gtcggcggtt gttggtttgc
    17161 ctggcgtcat cagtccagcg acgaaaacat tgattatttt gccgtttcgc tacgcatcat
    17221 tggcgttttg gcgctcatcc ttacctcctg tggtctggcg gcaatcaacg ctgacgatat
    17281 ctggtatttt gcctccggtg gcgtcattgg cagcttacta agcactacgc tacaaccact
    17341 gctacacagt agcgggggaa ctattgcgct gctctgcgtt tgggcagcgg gcctgacgtt
    17401 gttcaccggt tggtcatggg tgaccattgc tgaaaaactc ggcggctgga ttttaaacat
    17461 tctcaccttc gccagtaatc gtacccgtcg cgatgatacc tgggtcgatg aagatgagta
    17521 tgaagacgac gaagagtatg aagatgaaaa ccacggcaaa cagcatgaat cacgccgtgc
    17581 ccgtattctt cgcggcgcgc tagcgcgtcg taaacggttg gcggaaaaat tcattaatcc
    17641 gatggggcgg caaacagacg ctgcgttgtt ctccggtaag cggatggatg atgacgaaga
    17701 gattacctac actgcacgcg gtgtggctgc cgacccggac gacgtcctat tttcgggcaa
    17761 tcgtgcaacg cagccagaat atgacgaata cgatccatta ttaaacggtg cgccaattac
    17821 cgaacctgtc gctgtagcag ctgctgctac cacggcgaca caaagctggg ctgcgccggt
    17881 tgaacctgtg actcagacgc cgcctgttgc ctctgttgat gttccacctt cgcaacctac
    17941 agtagcctgg cagcctgtac cgggtccaca aacgggagag ccggttattg ctcctgcacc
    18001 ggaaggttac ccacagcagt cacaatatgc gcagcctgca gtgcaatata atgagccgct
    18061 gcaacaacca gtacagccgc agcagccgta ttatgcacct gcagctgaac aacctgcgca
    18121 acagccgtat tatgcccctg cgccagaaca accggtggca ggtaacgcct ggcaagccga
    18181 agagcagcaa tccacttttg ctccacagtc tacataccag actgagcaaa cttatcagca
    18241 gccagccgct caggagccgt tgtaccaaca gccgcaaccc gttgaacagc agcctgttgt
    18301 ggagcctgaa cccgttgtag aagagacaaa acccgcgcgt ccgccgcttt actactttga
    18361 agaagttgaa gagaagcgag cccgtgaacg tgaacaactt gcggcctggt atcaaccgat
    18421 tccagaaccg gttaaagaac cagaaccgat caaatcttcg ctgaaagcac cttctgttgc
    18481 agcagtacct ccagtagaag ccgctgccgc tgtttctccg ctggcatctg gcgtgaaaaa
    18541 agcgacactg gcgacggggg ctgccgcaac cgttgccgcg ccagtcttca gtctggcaaa
    18601 tagcggtgga ccgcgtccgc aggtcaaaga ggggattggc ccgcagttgc cacgaccgaa
    18661 acgtatccgc gtgccaactc gtcgtgaatt ggcgtcttac ggtattaagc tgccctcaca
    18721 gcgtgcggcg gaagaaaaag cccgtgaagc ccagcgcaat cagtacgatt ctggcgatca
    18781 gtacaacgat gatgaaatcg atgcgatgca gcaggatgaa ctggcacgtc agttcgccca
    18841 gacacagcag caacgctatg gcgaacagta tcaacatgat gtgcccgtaa atgcagaaga
    18901 tgcagatgct gcggcagagg ctgaactggc tcgtcagttt gcgcaaactc aacaacaacg
    18961 ttattccggc gaacaaccgg ctggggcgaa tccgttctcg ctggatgatt ttgaattttc
    19021 gccaatgaaa gcgttgctgg atgatggtcc acacgaaccg ctgtttacgc caattgttga
    19081 acctgtacag cagccgcaac aaccggttgc accgcagcag cagtatcagc agccacaaca
    19141 gccggttgcg ccacagccgc agtatcagca gccacaacag caggttgcgc cgcagccaca
    19201 atatcagcag ccgcaacaac cggttgcgcc acagcagcaa tatcagcagc cgcaacaacc
    19261 agttgcgccg cagccgcagt atcaacagcc gcaacaaccg gttgcaccgc agccgcagta
    19321 tcagcagcca caacagccag ttgcgccaca gccgcaggat actctgcttc atccgctgtt
    19381 gatgcgtaat ggcgacagcc gtccgttgca taaaccgacg acgccgctgc cttctctgga
    19441 tttgctgaca ccgccgccga gcgaagtgga gccggtagat acctttgcgc ttgaacaaat
    19501 ggctcgcctg gtggaagcgc gtctggctga tttccgtatt aaagccgatg tcgtcaatta
    19561 ctctccgggg ccggttatca ctcgctttga acttaacctg gcaccgggcg taaaagcggc
    19621 gcgcatttct aacttgtcac gggaccttgc ccgttcactt tcgacggtgg cggtgcgtgt
    19681 cgttgaagtt attcctggca aaccctatgt aggtctggag ttaccgaata aaaaacgaca
    19741 aaccgtttat ctgcgcgaag tattggataa cgccaaattc cgcgataatc catcgccatt
    19801 aaccgtggtg ctgggtaaag atatcgccgg tgagccggtg gttgccgatc tggcgaaaat
    19861 gccgcacttg ttagttgcgg ggactaccgg ttccggtaaa tctgtcggtg tgaacgcgat
    19921 gatcctgagc atgctttata aagcacagcc agaagatgtg cgtttcatca tgatcgaccc
    19981 gaaaatgctg gagctttcgg tgtatgaagg cattccgcac ctgttaacgg aagtcgttac
    20041 tgatatgaaa gatgccgcca acgcgctgcg ctggtgtgtt aacgagatgg agcgtcgtta
    20101 taaactgatg tctgcgctgg gtgtgcgtaa tctggcgggt tataacgaaa aaattgctga
    20161 agccgatcgc atgatgcgtc cgattccaga cccgtactgg aagccgggtg acagtatgga
    20221 tgcccagcat ccggtgctga aaaaagaacc atacattgtg gtgttggtgg acgaatttgc
    20281 cgacctgatg atgacagtag gtaaaaaagt ggaagagctg atagcacgtc tggcgcaaaa
    20341 agcccgtgcc gcgggtatcc acctcgtact ggcaactcag cgtccatcgg ttgatgttat
    20401 tactggtctg attaaagcga atattccgac ccgtatcgcc tttaccgtat ccagtaagat
    20461 tgactcacgt accattcttg atcaggctgg cgcggaatca ctgctgggta tgggggatat
    20521 gctctactct gggccgaact ccacgttgcc ggtacgtgtc catggtgctt ttgttcgcga
    20581 tcaggaagtt catgccgtgg tgcaggactg gaaagcgcgt ggtcgcccac agtatgttga
    20641 tggcatcacc tccgacagcg aaagcgaagg tggtgcgggt ggtttcgatg gcgctgaaga
    20701 actggatccg ttgttcgatc aggcggtgca gtttgtcact gaaaaacgca aagcgtcaat
    20761 ttctggcgta caacgtcagt tccgcattgg ttataaccgt gcagcgcgta ttatcgaaca
    20821 gatggaagcg caggggattg tcagcgaaca ggggcacaac ggtaatcgtg aagtgctgtc
    20881 cccaccgccg tttgactaat taatgcattg ccggataagg cgcggtagcg tcgcatccgg
    20941 cactctatca actgaaaatt cagtattttc ttctttcctc aagctgatta ttagcctgga
    21001 atagagagta gagggaactc ccgatcggga gtgacgtaat ttgaggaata atgatgaaaa
    21061 aaattgccat cacctgtgca ttactctcaa gcttagtagc aagcagcgtt tgggctgatg
    21121 ccgcaagcga tctgaaaagc cgcctggata aagtcagcag cttccacgcc agcttcacac
    21181 aaaaagtgac tgacggtagc ggcgcggcgg tgcaggaagg tcagggcgat ctgtgggtga
    21241 aacgtccaaa cttattcaac tggcatatga cacaacctga tgaaagcatt ctggtttctg
    21301 acggtaaaac actgtggttc tataacccgt tcgttgagca agctacggca acctggctga
    21361 aagatgccac cggtaatacg ccgtttatgc tgattgcccg caaccagtcc agcgactggc
    21421 agcagtacaa tatcaaacag aatggcgatg actttgtcct gacgccgaaa gccagcaatg
    21481 gcaatctgaa gcagttcacc attaacgtgg gacgtgatgg cacaatccat cagtttagcg
    21541 cggtggagca ggacgatcag cgcagcagtt atcaactgaa atcccagcaa aatggggctg
    21601 tggatgcagc gaaatttacc ttcaccccgc cgcaaggcgt cacggtagat gatcaacgta
    21661 agtagaggca cctgagtgag caatctgtcg ctcgattttt cggataatac ttttcaacct
    21721 ctggccgcgc gtatgcggcc agaaaattta gcacagtata tcggtcagca acatttgctg
    21781 gctgcgggga agccgttgcc gcgcgctatc gaagccgggc atttacattc tatgatcctc
    21841 tgggggccac cgggtaccgg caaaacaacc ctcgctgaag tgattgcccg ctatgcgaac
    21901 gctgatgtgg aacgtatttc tgccgtcacc tctggtgtga aagagattcg cgaggcgatc
    21961 gagcgcgccc ggcaaaaccg caatgcaggt cgccgcacta ttctttttgt tgacgaagtt
    22021 caccgtttca acaaaagcca gcaggatgca tttctgccac atattgaaga cggcaccatc
    22081 acttttattg gcgcaaccac tgaaaacccg tcgtttgagc ttaattcggc actgctttcc
    22141 cgtgcccgtg tttatctgtt gaaatccctg agtacagagg atattgagca agtactaact
    22201 caggcgatgg aagacaaaac ccgtggctat ggtggtcagg atattgttct gccagatgaa
    22261 acacgacgtg ccattgcaga actggtgaat ggcgacgcgc gccgggcgtt aaatacgctg
    22321 gaaatgatgg cggatatggc cgaagtcgat gatagcggta agcgggtcct gaagcctgaa
    22381 ttactgaccg aaatcgccgg tgaacgtagc gcccgctttg ataacaaagg cgatcgcttt
    22441 tacgatctga tttccgcact gcataagtcg gtacgtggta gcgcacccga tgcggcgctg
    22501 tactggtatg cgcgaattat taccgctggt ggcgatccgt tatatgtcgc gcgtcgctgt
    22561 ctggcgattg cgtctgaaga cgtcggtaat gccgatccac gggcgatgca ggtggcaatt
    22621 gcggcctggg attgctttac tcgcgttggc ccggcggaag gtgaacgcgc cattgctcag
    22681 gcgattgttt acctggcctg cgcgccaaaa agcaacgctg tctacactgc gtttaaagcc
    22741 gcgctggccg atgctcgcga acgcccggat tatgacgtgc cggttcattt gcgtaatgcg
    22801 ccgacgaaat taatgaagga aatgggctac gggcaggaat atcgttacgc tcatgatgaa
    22861 gcaaacgctt atgctgccgg tgaggtttac ttcccgccgg aaatagcaca aacacgctat
    22921 tatttcccga caaacagggg ccttgaaggc aagattggcg aaaagctcgc ctggctggct
    22981 gaacaggatc aaaatagccc cataaaacgc taccgttaat gttatcgttg cggtaatgtt
    23041 gttactgtat ccctgtggtc gcaggctgtg gccacatctc ccatttaatt cgataagcac
    23101 aggataagca tgctcgatcc caatctgctg cgtaatgagc cagacgcagt cgctgaaaaa
    23161 ctggcacgcc ggggctttaa gctggatgta gataagctgg gcgctcttga agagcgtcgt
    23221 aaagtattgc aggtcaaaac ggaaaacctg caagcggagc gtaactcccg atcgaaatcc
    23281 attggccagg cgaaagcgcg cggggaagat atcgagcctt tacgtctgga agtgaacaaa
    23341 ctgggcgaag agctggatgc agcaaaagcc gagctggatg ctttacaggc tgaaattcgc
    23401 gatatcgcgc tgaccatccc taacctgcct gcagatgaag tgccggtagg taaagacgaa
    23461 aatgacaacg ttgaagtcag ccgctggggt accccgcgtg agtttgactt tgaagttcgt
    23521 gaccacgtga cgctgggtga aatgcactct ggcctcgact ttgcagctgc agttaagctg
    23581 actggttccc gctttgtggt aatgaaaggg cagattgctc gcatgcaccg cgcactgtcg
    23641 cagtttatgc tggatctgca taccgaacag catggctaca gtgagaacta tgttccgtac
    23701 ctggttaacc aggacacgct gtacggtacg ggtcagctgc cgaaatttgc tggcgatctg
    23761 ttccatactc gtccgctgga agaagaagca gacaccagta actatgcgct gatcccaact
    23821 gcagaagttc cgctgactaa cctggtgcgc ggtgaaatca tcgatgaaga tgatctgcca
    23881 attaagatga ccgcccacac cccatgcttc cgttctgaag ccggttcata tggtcgtgac
    23941 acccgtggtc tgatccgtat gcaccagttc gacaaagttg aaatggtgca gatcgtgcgc
    24001 ccggaagact caatggcggc gctggaagag atgactggtc atgcagaaaa agtcctgcag
    24061 ttgctgggcc tgccgtaccg taaaatcatc ctttgcactg gcgacatggg ctttggcgct
    24121 tgcaaaactt acgacctgga agtatggatc ccggcacaga acacctaccg tgagatctct
    24181 tcctgctcca acgtttggga tttccaggca cgtcgtatgc aggcacgttg ccgcagcaag
    24241 tcggacaaga aaacccgtct ggttcatacc ctgaacggtt ctggtctggc tgttggtcgt
    24301 acgctggttg cagtaatgga aaactatcag caagctgatg gtcgtattga agtaccagaa
    24361 gttctgcgtc cgtatatgaa cggactggaa tatattggct aatacccaat ttttctgaat
    24421 ctaaaaagcg cctacgggcg ctttttttgt ctccctttga taccgaacaa taattactcc
    24481 tcacttacac gtaatactac tttcgagtga aaatctacct atctctttga ttttcaaatt
    24541 attcgatgta tacaagccta tatagcgatc tgctatagaa ataattacac aatacggttt
    24601 gttactggaa tcaatcgtga gcaagcttga gtgagccatt atgaaaacga aaatccctga
    24661 tgcggtattg gctgctgagg tgagtcgccg tggtttggta aaaacgacag cgatcggcgg
    24721 cctggcaatg gccagcagcg cattaacatt accttttagt cggattgcgc acgctgtcga
    24781 tagcgccatt ccaacaaaat caggcgaaaa ggttatctgg agcgcctgta cagttaactg
    24841 tggtagtcgc tgcccgctac gtatgcacgt cgtggacggt gaaatcaaat atgtcgaaac
    24901 ggacaatacc ggcgatgaca attacgacgg cctgcaccag gttcgcgcct gcctgcgtgg
    24961 gcgttccatg cgtcgccgtg tctacaatcc ggaccgcctg aaatatccga tgaaacgagt
    25021 cggggcgcgc ggtgaaggca aattcgagcg cattagctgg gaagaagcct acgacatcat
    25081 cgcgaccaat atgcagcgcc tgatcaaaga gtacggcaac gaatccatct atctgaacta
    25141 tggcaccggt acgctgggtg gcaccatgac ccgctcctgg ccgccgggaa ataccctggt
    25201 cgcgcggctg atgaactgct gcggcggcta tctgaaccat tacggcgact actcctccgc
    25261 gcaaattgcg gaaggtctga actataccta cggcggctgg gcagatggca acagcccgtc
    25321 ggatatcgaa aacagtaagc tggtagtgct gtttggtaat aaccctggcg aaacgcgaat
    25381 gagtggcggt ggggtgactt actatcttga acaggcacgc cagaaatcta atgcccgcat
    25441 gatcatcatc gatccgcgct ataccgacac cggtgccggg cgcgaagatg agtggatccc
    25501 tattcgtccg ggaacagatg ccgcactggt taacggtctg gcgtacgtca tgatcactga
    25561 aaacctggtg gatcaggcat tcctcgataa atattgcgtt ggctacgatg agaaaaccct
    25621 gccagccagt gcgccgaaaa atggccacta taaagcttat attctgggtg aagggccaga
    25681 tggcgtggct aaaacaccgg aatgggcctc gcaaatcacc ggtgttccgg cagacaaaat
    25741 catcaaactg gctcgtgaaa tcggcagtac caaaccggcg tttatcagcc agggatgggg
    25801 cccgcagcgt cacgctaacg gtgaaatcgc aacccgtgct atctcgatgc tggcgattct
    25861 gaccggtaac gttggtatta atggagggaa cagcggcgcg cgtgaaggtt catacagttt
    25921 accgtttgtc cgtatgccga ccttggaaaa cccgatccag accagcattt cgatgtttat
    25981 gtggaccgat gccattgaac gtggcccgga aatgacggcg ctacgtgatg gtgtgcgcgg
    26041 gaaagataag ctggatgtgc cgatcaaaat gatctggaac tatgccggta actgcctgat
    26101 taaccagcat tctgaaatca accgtaccca tgaaatcctt caggatgata agaagtgcga
    26161 gctgattgtg gttatcgact gccacatgac ctcatcggcg aaatatgctg acatcctgct
    26221 gcctgactgc accgcttccg aacagatgga ctttgcactg gatgcatcct gcgggaatat
    26281 gtcttacgtg attttcaacg atcaggtgat taaaccgcgc tttgaatgta agaccatcta
    26341 tgaaatgacc agcgaactgg caaaacgtct tggcgttgag caacagttta ctgaaggccg
    26401 tacccaggaa gagtggatgc ggcatctgta tgcccagtcg cgggaagcga ttcctgaact
    26461 gccaacgttt gaagagttcc gcaagcaggg gatctttaaa aagcgcgacc cacaagggca
    26521 tcacgttgct tataaagcct tccgtgaaga tccgcaggca aatccactga ccacgccatc
    26581 gggtaaaatt gagatttatt cgcaggcgct ggctgacatt gccgctacct gggaattgcc
    26641 agaaggcgat gtgatcgatc cactgccgat ctacacgccg ggctttgaaa gttatcagga
    26701 tccgctgaac aaacagtatc cgctgcagct tacaggtttc cactataaat ctcgcgttca
    26761 ctcaacttac ggcaacgttg atgtgctgaa agcggcttgc cgtcaggaaa tgtggatcaa
    26821 cccgcttgat gcccaaaaac gcggtatcca caacggcgat aaagtcagga tctttaacga
    26881 tcgtggtgag gttcatattg aggcgaaagt gacgccacga atgatgccgg gtgtggtcgc
    26941 actgggtgaa ggtgcctggt atgacccgga tgcaaaacgt gtcgataagg gtggttgtat
    27001 taacgtactg accactcaac gtccgtctcc tctcgctaag gggaatccgt cacatacaaa
    27061 ccttgttcag gttgaaaagg tgtaaggagt aaccgatgac aacccagtat ggatttttta
    27121 ttgattccag ccgttgcacc ggttgcaaaa cctgcgagct ggcttgtaaa gactacaaag
    27181 atttgatgcc agaagtcagc ttccgccgca tttatgaata tgctggtggc gactggcagg
    27241 aagataacgg tgtctggcac cagaacgtgt ttgcctacta tctgtcgatt tcgtgcaacc
    27301 actgcgaaga tccggcttgt actaaagtct gcccgagcgg tgcgatgcat aaacgtgaag
    27361 atggttttgt tgtggtcgat gaagatgtgt gcattggctg ccgctactgc catatggctt
    27421 gcccgtatgg cgcaccgcaa tataacgaaa cgaaaggcca tatgaccaaa tgcgatggtt
    27481 gttatgaccg tgttgcggag ggtaaaaagc cgatctgtgt tgaatcttgt ccgctgcggg
    27541 cgctggattt cggccctatc gacgagctgc gtaaaaaaca tggcgatctg gcggccgttg
    27601 cgccgttgcc gcgagctcac tttaccaaac cgaatattgt gatcaaaccc aatgccaata
    27661 gccgcccgac cggggatacc actggctatc tggcaaaccc gaaggaggtg tgagatggga
    27721 agtggatggc atgaatggcc gctgatgatc ttcacggtct tcgggcaatg tgtggcgggt
    27781 ggttttatcg ttctggcttt ggcgctgctt aaaggcgacc tgcgagcaga agcccagcag
    27841 cgtgttatcg cctgcatgtt tggcttatgg gtgctgatgg gcattggctt tatcgcctct
    27901 atgctccatc ttggttcacc gatgcgcgcc tttaactcgc tcaaccgggt aggggcttca
    27961 gcactcagta acgaaatcgc cagcggttcg atcttttttg ctgtaggcgg catcggctgg
    28021 ttgctggcaa tgctgaaaaa gctgtcacct gcattgcgta cgctatggct ggtggtgacg
    28081 atggttcttg gcgtcgtctt tgtctggatg atggtgcgtg tgtataacag cattgatacc
    28141 gttccgacct ggtacagcat ctggacgccg atgggcttct tcctgacgat gttcatgggc
    28201 ggcccgttac tgggttacct gttgctgagt ctggctggcg tcgatggctg ggcgatgcgt
    28261 ctgctgccag ccatttctgt actggcactg gtggtgagtg gcgtgatgtc agtgatgcag
    28321 ggcacagagc tggcaactat tcatagttct gtgcagcagg ccgcagcgct ggtgccggac
    28381 tatggtgcgc tgatgtcctg gcgaatcgtg cttttggccg ttgccctgtg cttgtggatt
    28441 gcaccacagc taaaaggtta tcagcctgcg gttccgctac tatccgtctc attcattctg
    28501 cttctggcag gggaattaat cggtcgcggc gtattctatg gtttgcatat gaccgtgggg
    28561 atggccgtcg caagctaatc agtaacaacc ggggtttcgg ccccggttct cttttatttc
    28621 tgcttcgtta acgtgtcata actggtcatc aaattacgat aatcaggaat gtggttagag
    28681 aacaacgtcg ccagtccttc aatatcatta cgccagtcac ggtgcagctc gcaagccacg
    28741 ccgaaccatg tcatcaattg cgcacctgct tgcgacatgc gatcccatgc cgaatgccgg
    28801 gtaatttcat taaaggtccc tgacgcgtca gtcacaacaa agacatcaaa cccttcttca
    28861 atggctgaaa gcgccgggaa tgccacgcaa acttcggtta ccacaccggc aataattaac
    28921 tgttttttac ctgtcgcttt gactgctttt acaaaatctt cgttatccca ggcgttaata
    28981 ttgccagggc gagcaatgta aggtgcatcg gggaattgtg ctttcagctc tggaactaat
    29041 gggccgttag gaccggtttc aaaactggtg gtaagaatag ttggtaagtt gaaatactta
    29101 gccaggtcac ccagcgccag cacgttattt ttaaacttat cgggttcgat atcccgtaca
    29161 agggaaagta aaccagcctg gtgatcaaca agcaaaacgg cagcatcatt tttatcaaga
    29221 cgaacatacg gtttggtcat cctcttctcc tttcgagagt cagtggtacg tctgaaatcg
    29281 gtcagacgcg atgcattgct ctgaaagcat agacgggaaa tatgagtttg ctgtgaccat
    29341 gaaatttttc gactgaacga agcgtatgaa atttgtgtta gatcaataaa aacaatcaga
    29401 tgagattaat catctcttca gcattagcgt tatgaatgag taatataaat cgataatacc
    29461 gccgctgcga tatgcgcgta ttttcaccca ttgacaatgt tttttgcggt ggcatgatgc
    29521 gcatgaaatt tgaacttcct cacggtttta attcatgtcc acgtataccc ggcctgtcat
    29581 gcttttgctg tctggcctgc ttttgttgac tctggcgatc gcggtgttaa atacactcgt
    29641 gccgctttgg ctcgcccagg aacacatgtc cacatggcag gtaggcgttg tcagctcatc
    29701 ctattttacc ggcaaccttg tcggtacatt gctgacaggg tatgtcatta aacgcattgg
    29761 ctttaaccgc agctattatc tggcctcctt catttttgcc gctggctgtg ccggccttgg
    29821 cctgatgatt ggtttctgga gctggttggc gtggcgtttt gtcgcgggcg tcggctgtgc
    29881 catgatttgg gtagtcgttg agagcgcgct gatgtgcagt gggacgtcac gtaaccgtgg
    29941 gcgtttgctt gctgcgtata tgatggttta ttacgtggga acatttttag gccagttact
    30001 ggtcagcaaa gtctcaaccg agctgatgag cgtattgccg tgggttacag gtttgacgtt
    30061 ggcagggatc ttaccgctgt tgtttacgcg tgtcctgaat cagcaggctg aaaaccatga
    30121 ttcgacgtca attacgtcaa tgctaaaact ccgtcaggcg cggcttggcg tgaatggctg
    30181 cattatctca ggaatcgtgc tgggatctct atatggcctg atgccgctgt acctcaatca
    30241 caaaggggtg agcaatgcca gcattggttt ctggatggcg gtactggtca gtgcgggtat
    30301 cctcggacaa tggccgattg gacgtctggc ggataagttt ggtcgactgc tggtgttgcg
    30361 tgttcaggtc tttgtcgtca ttctcggcag tatcgcgatg cttagccagg cggcgatggc
    30421 cccagcgtta ttcatcctcg gtgccgctgg ctttacgcta tatccggtgg cgatggcctg
    30481 ggcttgtgag aaagttgaac atcatcaact ggtggcgatg aaccaggcct tactgttgag
    30541 ctataccgtg ggaagtctgc ttggcccgtc atttaccgct atgctaatgc agaatttctc
    30601 cgataattta ttgtttatca tgatcgccag cgtatcgttt atctatttgc tgatgctgct
    30661 tcgcaacgcc ggtcatacgc cgaaacccgt tgctcacgtg taaatgaatt caagcaaagt
    30721 gtgaacttac tgtttcac
//
LOCUS       DBFIFM010000039        29530 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.039, whole genome shotgun sequence.
ACCESSION   DBFIFM010000039 DBFIFM010000000
VERSION     DBFIFM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.039"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..1178)
                     /locus_tag="N7C74_001520"
     CDS             complement(<1..1178)
                     /locus_tag="N7C74_001520"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HGU2431416.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKEGEDPGYTL
                     "
     gene            complement(1389..2786)
                     /gene="ccp"
                     /locus_tag="N7C74_001521"
     CDS             complement(1389..2786)
                     /gene="ccp"
                     /locus_tag="N7C74_001521"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HGU2431417.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLDVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            3191..4840
                     /locus_tag="N7C74_001522"
     CDS             3191..4840
                     /locus_tag="N7C74_001522"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HGU2431418.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(4891..5493)
                     /locus_tag="N7C74_001523"
     CDS             complement(4891..5493)
                     /locus_tag="N7C74_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGU2431419.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGIMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            6013..6912
                     /locus_tag="N7C74_001524"
     CDS             6013..6912
                     /locus_tag="N7C74_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2431420.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPHLSEFLYQHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQNPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            6961..7974
                     /gene="yhjD"
                     /locus_tag="N7C74_001525"
     CDS             6961..7974
                     /gene="yhjD"
                     /locus_tag="N7C74_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HGU2431421.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            8386..9708
                     /locus_tag="N7C74_001526"
     CDS             8386..9708
                     /locus_tag="N7C74_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2431422.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLSAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(9890..11950)
                     /locus_tag="N7C74_001527"
     CDS             complement(9890..11950)
                     /locus_tag="N7C74_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HGU2431423.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGIAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(12020..12787)
                     /gene="pdeH"
                     /locus_tag="N7C74_001528"
     CDS             complement(12020..12787)
                     /gene="pdeH"
                     /locus_tag="N7C74_001528"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HGU2431424.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTAVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            13019..13948
                     /gene="kdgK"
                     /locus_tag="N7C74_001529"
     CDS             13019..13948
                     /gene="kdgK"
                     /locus_tag="N7C74_001529"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HGU2431425.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESDQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(14044..15540)
                     /locus_tag="N7C74_001530"
     CDS             complement(14044..15540)
                     /locus_tag="N7C74_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGU2431426.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(15761..17047)
                     /gene="dctA"
                     /locus_tag="N7C74_001531"
     CDS             complement(15761..17047)
                     /gene="dctA"
                     /locus_tag="N7C74_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HGU2431427.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDALNNRAPDGKTHELSS"
     gene            complement(17230..19218)
                     /gene="hmsP"
                     /locus_tag="N7C74_001532"
     CDS             complement(17230..19218)
                     /gene="hmsP"
                     /locus_tag="N7C74_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HGU2431428.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVHEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSISVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSVLQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(19300..22773)
                     /gene="bcsC"
                     /locus_tag="N7C74_001533"
     CDS             complement(19300..22773)
                     /gene="bcsC"
                     /locus_tag="N7C74_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HGU2431429.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWSSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWALQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLRDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(22755..23861)
                     /gene="bcsZ"
                     /locus_tag="N7C74_001534"
     CDS             complement(22755..23861)
                     /gene="bcsZ"
                     /locus_tag="N7C74_001534"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HGU2431430.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(23868..26243)
                     /gene="bcsB"
                     /locus_tag="N7C74_001535"
     CDS             complement(23868..26243)
                     /gene="bcsB"
                     /locus_tag="N7C74_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HGU2431431.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAVLAVLAVLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(26254..28872)
                     /gene="bcsA"
                     /locus_tag="N7C74_001536"
     CDS             complement(26254..28872)
                     /gene="bcsA"
                     /locus_tag="N7C74_001536"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HGU2431432.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGGALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTVGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(28869..29530)
                     /gene="bcsQ"
                     /locus_tag="N7C74_001537"
                     /pseudo
     CDS             complement(28869..29530)
                     /gene="bcsQ"
                     /locus_tag="N7C74_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
BASE COUNT         6981 a         8169 c         7440 g         6940 t
ORIGIN      
        1 tacagggtgt atcccggatc ttcaccctct ttcagtttga agcaaaccgc cgggatgcct
       61 tcgtccggac gacccgtaca gatgaactca tacggcccca gtttggcgat ttcatccgcc
      121 agataagcgg caacctggta agaggcgttc tgtactttgg tatagccttc acgaccgagg
      181 cgcaggaatt cataatactg cgcaatcacc tgacccgccg ggcgggagaa gttgatggcg
      241 aaagtaccaa tctgaccacc gaggtagtca acgttgaaca ccagttcctg cggcagcgct
      301 tcttcatcac gccagatcac ccagccgcag cccagcggag ccagaccgaa tttatggcct
      361 gaagcactga tcgatttcac acgcggcagg cggaagtccc agacgatatc cggggcgacg
      421 aacggtgcca ggaagccacc gctggcagcg tcgatgtgca tgtcgatgtc gataccggtg
      481 tcggcctgga atttatccag tgcatcgtgc agcggttgcg ggaactcata gttaccggtg
      541 taggtcacgc cgaaagtcgg caccacgccg atggtgtttt cgtcgcaggc ttcaatcatg
      601 cgtttcgggt ccataaacaa ctgaccgggg cgcataggga tctcgcgcag ctccacatcc
      661 cagtagcgag cgaatttatg ccagcagatt tgcaccggac cgcacaccag gtttggttta
      721 tccgttggtt tgcctgcagc ttccatacgc ttgcgccaac gccatttcat cgccatcccg
      781 ccaagcatac aagcctcgga agaaccaatg gtgttggtgc caacggcctg accatttttc
      841 ggcgcaggcg catgccacag atcggcaacc atgtttacgc aacgcaggtc gatggctgcg
      901 gattgcggat attcttcttt gtcgatccag tttttattga tcgacaaatc catcaattta
      961 tggacgtttt cgtcgtccca ggtctggcag aaagtggcca ggttctgacg agcgttgcca
     1021 tcaagatata attcatcatt gataatctgg aatgcgacat catcgcgcat ttcgtgcagc
     1081 ggaaatcgtt ttgactccgc gatagtagaa atggcctttg cgccaaaacg tgaatcgagt
     1141 agttctgagc ggaaatccgt taacagcttc tggtccattt cgaactcctt aaatttattt
     1201 gaaggcaata aaaaagtagg atttatccgc aatggaagca aggcattaca ggcttaattt
     1261 aaataacaaa atcctaagca gaaaaatgat cttttataat ataaacattt aatatgataa
     1321 tattaaaaac agactttatt atcgtaataa aaatcgccca aacattcctg ttcgggcgat
     1381 caaattaatt attgtttatc ctgcatatac ggcgtgtaaa ccccgttcag actgtgcagg
     1441 aaagctacga tatcatccac atcctcctgc ggcagctctt tgcctacctg atagcgcaac
     1501 atcagtttca ccgccccgtc cagcgtcggc acgtcaccgc gatggaagta cggtgccgtt
     1561 aaagcaacgt tacgtaaacc gggtactttc tgacgcaatt tatcacgctc ttctttagtc
     1621 acattcatac gaccaatatc cgccgccgta atttccccaa agttaaagtc ttttttcagc
     1681 cccaacggtt cgaaggaacg tccgccgaga ataattccac catgacaagt tgcacattta
     1741 ttatctttaa ataattgata gccttttttc tgttgcgccg tcagcgcatt ttcatcccca
     1801 cgcaaccatt tatcaaatgg ggaatccggc gtaattaatg ttttctcaaa ttcagcaatg
     1861 gcatcagtaa tattttcgcc actgaaacct tgcggataga cgtcgaggaa ctgcgcttta
     1921 agctgcggat ctttttccag cttagcaata atttcgtccc aggattttga cgccatttca
     1981 atcgggttca acggcggtcc accagcctga tcctgcaatg ttgccgcacg accatcccag
     2041 aactgctcaa cgttaaatac tgagttaaat accgtcggcg cgttaatcgg cccaactgcg
     2101 ccaccaacac caatcgatgt ttttctgcca tcgacgcccc ccgcattcaa cgcatggcaa
     2161 tgagcgcatg aaatggtgct atcagccgat aaacggggat cgtgatacag cgcaaagccc
     2221 aacgccactt tttgcgcatc ggtaggcagt ttttgcggga tgggctgcac cggttcattg
     2281 cgatgctccg gagcagtatc attgctggcg taatattccg cgcgctgttt tgcaatccag
     2341 gccagtattt ccgcccgctc ttcatcactc accttacccg cccagtgtag cgcggtataa
     2401 cgcgttggcg gcatagtttc atactgcatc acccattcaa tcttattcag atcgctttgc
     2461 gaaacgggtt tatcagccag cagagcagca cgcacggcct cgaggttaaa agatttatat
     2521 ccaagcttaa tgtcgtaatc catcaactgt ttcgcgccag gaatataata ataggcgggt
     2581 aattctgccg aaggcgtgtg gcaatagtcg catccttttt cgcggagaaa gcctaaaacc
     2641 ttattatttt cactgacagc agatgcctga acatcggcct gtttactgcg tttattatcg
     2701 tggtaccaca cataaccaga taaccctaaa tagcaaatcg cgacgccagc caggccgatc
     2761 gcggtaatac gtgagaccat tttcattatt cttccctcac ggtggtgagt tattgtgacc
     2821 cagaaaattt tccagaatgc tgcaaacgtc tctgcgagac aaagccatca taaaaaacag
     2881 ggagatacgc tttttgatag gaatcaatca attacatgaa tataaactat caataagata
     2941 gcctgtgcct attacccaga tcaggaatgg catatcatga ttgcggcaag agcaggattg
     3001 aatgttctga aaatgaaaaa caacacgaaa tctgtatttt tataaattcc attgatatta
     3061 gtgcgttata ttttttgaca ggttaataaa cgtgatccac cgcacgcttt gtcgcccacc
     3121 aggcggagcg aatgactacc cttaaagaaa agcccgataa ttagcgacga atttcggagg
     3181 ttggatcctt atgctcaatc agaaaattca aaaccctaat ccagacgaac tgatgatcga
     3241 ggtcgatctc tgctatgaac tggacccgta tgaattaaaa ctggatgaga tgatcgaggc
     3301 agaaccggaa cccgagatga ttgaagggct gcccgcctct gatgcgctga cgcctgccga
     3361 tcgctatctc gaactgttcg agcatgttca gtcggcgaaa attttccccg acagtaaaac
     3421 ctttcccgac tgcgcaccca aaatggaccc gctggatatt ttaatccgct accgtaaagt
     3481 gcgccgtcat cgtgattttg acttgcgcaa gtttgttgaa aatcacttct ggctgccgga
     3541 ggtctactcc agcgagtatg tatcggaccc gcaaaattcc ctgaaagagc atatcgacca
     3601 gctgtggccg gtgctaaccc gcgaaccaca ggatcacatt ccgtggtctt ctctgctggc
     3661 gctgccgcag tcatatattg tcccgggcgg ccgttttagc gaaacctact attgggattc
     3721 ctatttcacc atgctggggc tggcggaaag tggtcgggaa gatttgctga aatgcatggc
     3781 cgataacttc gcctggatga tcgaaaacta cggtcacatc cccaacggca accgcaccta
     3841 ttatttgagc cgctcgcaac caccggtttt tgcgctgatg gtggagttgt ttgaagaaga
     3901 tggtgtacgc ggtgcgcgcc gctatctcga ccacctgaaa atggaatatg ccttctggat
     3961 ggacggtgca gaatcgttga tccctaatca ggcctatcgc catgttgtgc ggatgccgga
     4021 cggatcgctg ctcaaccgtt actgggacga tcgcgacacg ccgcgtgacg aatcctggct
     4081 tgaggacgtt gaaaccgcga aacattctgg tcgcccgccc aacgaggtgt accgcgattt
     4141 acgcgcgggg gcggcctccg gttgggatta ctcttcccgt tggctccgtg atactggtcg
     4201 tctggcgagc attcgtacca cccagttcat ccccatcgat ctgaatgcct tcctgtttaa
     4261 actggagagc gccatcgcca acatctcggc gctgaaaggt gagaaagaga cagaagcact
     4321 gttccgccag aaggccagtg cccgtcgcga tgcggtaaac cgttacctct gggatgatga
     4381 aaacggcatc taccgcgatt acgactggcg acgcgaacaa ctggcgctgt tttccgctgc
     4441 cgccattgtg ccgctctatg tcggtatggc gaaccatgaa caggccgatc gtctggcaaa
     4501 cgccgtgcgc agtcggttac tgacacctgg cgggattctg gcaagcgagt acgaaaccgg
     4561 tgaacagtgg gataaaccca acggctgggc accgttacaa tggatggcaa ttcagggatt
     4621 taaaatgtat ggcgatgacc ttctgggcga tgaaatcgcg cgcagctggc tgaagacggt
     4681 gaatcagttt tatctggaac agcacaaact gatcgaaaaa taccatattg ccgatggtgt
     4741 tccccgcgaa ggcggcggtg gcgagtatcc gttgcaggat gggtttggct ggactaacgg
     4801 tgtggtacgc cgtttaattg gtttgtacgg cgaaccataa tatttttaca gccagccgct
     4861 aacttcctgc tggctgtaaa attatcctct tcaggaggag atatttaaca tcattgccgc
     4921 ctgggtgcga tttttcactt ccagacggcg atacagggat tccagatgcg cttttaccgt
     4981 tccggtactg atattcagcg ctctgccgat ctccttattt gattcgcccg ccgctaacat
     5041 ggttaaaatc tcccgctggc gggcacttaa cgatttgaga tctttaatgt ccttttccgg
     5101 cgtcgtccgc cagtctccag gcagaaacat tatccccatc gccgcactat ttaccgccaa
     5161 cgcaaatgtc tcgacggttg aatcacgagg cacaatggcc agcacattaa aatggataac
     5221 ttcctgtaac caccgtttat tgcagtccgt cgccgtaatt aacaccttaa cctcaggaaa
     5281 ttgcaccacg gttttttgca gcaaccagta gcaaaactca ccatcctgat cgccatcgag
     5341 cataactaag gcttcagggt aactttccag cttttgccat aactcgtctg cctgactggc
     5401 cccctgaata ctcactcctg gaatacgctg ctgtaaactg attttcattc catgaataaa
     5461 tattgactgc ctgtcaaaca tgactatttg cataactgaa tctccacctg aatacgttaa
     5521 aaagacttaa gtagtggaag ggtattaccc gcgagaaaaa ataagaattc gccatttggc
     5581 ggtggccatt ctacagagat ggcgcgtaga aaatagttac cgatataaat agttacagct
     5641 aaacgcctga aattacatgt cgagggcact atttaaaaca attttgagga tttccttata
     5701 ttggtggtta gtacgcatgc aattaaaaat gaaattccgc gaccacaagc caaaataaca
     5761 aatggcaatg agacaaaaat aagcacaaat agccaatatg tcctctattc actttaaagg
     5821 gaatcgctga aaaatacgct ctgtttaagg ggattcacct ttctcagaaa gctattccgc
     5881 ccttttcctg ctgagaaatc gccacattcg tcatgacaac attgtgaaac ccggcattag
     5941 atgttagaaa aaataataaa caatgcgata tgcgcgttac tgtttcgctg tgaacaacaa
     6001 tcggtcaaag aaatggataa aattcacgca atgcagttgt tcatcaaagt cgcggagctg
     6061 gaaagttttt cccgcgcagc ggatttcttt gctttgccaa aaggaagtgt ttcgcgccag
     6121 atacaggcac tggaacatca acttggcacc cagcttctcc agcgcaccac gcgacgggtc
     6181 aaactcacgc cagaaggcat gacctattat caacgagcaa aagatgtgtt gagtaatctc
     6241 aacgaactgg acggtctgtt tcagcaggat gccaccagta tcagcggtaa attacgcatc
     6301 gacatcccgc caggaatcgc aaaaagcctg ttactgccgc acctgtcgga atttctctat
     6361 cagcatccgg gaattgagct ggaactgagt agccatgacc gtccggtaga tattcttcat
     6421 gatggttttg attgcgtgat acgcactggc gcgttaccgg aagatggcgt tatcgcccgt
     6481 cccctcggca aactgaccat ggtcaactgt gccagtccgc actatctgac gcgctttggt
     6541 tatccgcaaa accccgacga tctcacttca cacgcaatag tgcgttatac accgcacctg
     6601 ggtgtacatc cgttaggttt tgaggttgcc agcgttaatg gcgtccagtg gtttaagtct
     6661 ggcggcatgt tgacggtaaa cagtagcgaa aactatctcg ccgccggtct tgccggtctg
     6721 gggattattc agatcccgcg cattgccgtg cgcgaagccc tgcgtgccgg gcggcttatt
     6781 gaagtgttgc caggctaccg tgccgagccg ctctcccttt cgctggttta tccgcagcgt
     6841 cgggagcttt cccggcgtgt aaacctgttt atgcagtggc tggctggcgt aatgaaagag
     6901 tacctggact gaccgactat actttttaag aatgaccacg acagaaggac aaaagagcgg
     6961 atgacgcagg aaaacgagat caaacgtccc acccaggatc tggagcacga gccgattaag
     7021 cagttggata atagcgagaa aggcggcaaa gttagccagg cgctggaaac cgtcaccacc
     7081 actgccgaaa aagtccaacg ccaaccggtt attgcgcacc tgattcgcgc gacagaacgc
     7141 tttaacgatc ggctgggtaa ccagtttggt gcggctatca cctatttctc atttttgtcg
     7201 atgatcccga ttttgatggt gtcgtttgcc gcagggggct ttgtgctggc ctcccatccg
     7261 atgttgctac aggatatctt cgacaaaatt ctgcaaaaca tcagcgatcc gacgctagcc
     7321 gctacgttga aaaacaccat caacaccgcc gttcagcagc gtacgactgt agggcttgtc
     7381 ggcctggcgg tggcgcttta ttccggcatc aactggatgg gtaacctgcg tgaagcgatt
     7441 cgtgcccagt cgcgcgatgt ctgggaacgc tcgccgcaag atcaggagaa gttctgggta
     7501 aaatatctgc gtgattttat ttcgctgatt ggtttgttga ttgcgctgat tgtgacgctt
     7561 tcgatcacct cggttgccgg ttcggcgcag caaatgatta ttagcgccct gcacctgaac
     7621 agcattgagt ggctgaaacc gacgtggcgg ttgattggcc tggcgatttc catcttcgcc
     7681 aactatctgc ttttcttctg gatcttctgg cggttgccgc gccaccgtcc gcgcaaaaaa
     7741 gccctgattc gcggaacatt tctggctgct attggttttg aagtgattaa aatcgtgatg
     7801 acctacaccc tgccatcgtt gatgaaatcc ccctctggcg cagcatttgg ttccgtgctg
     7861 gggctgatgg catttttcta cttcttcgcc cgtttgacgc tgttttgcgc ggcgtggatt
     7921 gccaccgccg aatataaaga cgacccgcga atgccgggga aaacgcagcc ttaaaatagg
     7981 ccggatgcag ttcaaaacgc cttatccggc atacgaattt attcggcctg gctccccgta
     8041 ggccgaataa gatgcgccag catcgcatcc ggctataatg cgcacataac ctcttgaaac
     8101 tctttcccag agcctcttca gccatctatt caggagcaaa caatttcatg ccaactcata
     8161 accccagcat ataaatccag ttggtaactt ttatttaacc tgaaaccagt tttatccact
     8221 atttataaaa ttatgtgaag catttcatag aagaaaaatc actggcctaa acattatccc
     8281 ctttttgcct ggtttttgac catttccgcg atttgttaca cattgaaata tcacttttgc
     8341 tgtgcgtaat atggctattc gttagccaaa aaataagaaa agattatgca agcaacagcc
     8401 acaacactcg accacgagca agaatacacg ccgatcaact cgcgtaataa agtccttgtc
     8461 gcctctctca ttggcacggc cattgagttc ttcgactttt acatttacgc cactgcggcc
     8521 gttattgtgt ttccgcatat cttcttcccg cagggcgatc ctacggcagc aacgctacag
     8581 tcgctcgcca ccttcgccat cgccttcgtc gcgcgcccca ttggctctgc cgtttttggt
     8641 cattttggcg atcgcgttgg gcgtaaagcg acgctggtcg cctcgttgct aacgatgggg
     8701 atttcgaccg tggtgattgg tctgctgcca ggctatgcca cgattggtat tttcgccccg
     8761 ctgctgctgg cgctggctcg atttggtcag ggtctgggct taggtggtga atggggcggc
     8821 gcggcgctgc tggcgactga aaacgcccca ccgcgcaaac gtgcattgta cggctccttc
     8881 ccgcagctgg gcgcaccgat tggcttcttc tttgccaacg gcactttctt gctgctttcc
     8941 tggctactga ccgacgagca gtttatgagc tggggctggc gtgtgccatt tatcttctcg
     9001 gcggtgctgg tcattatcgg cctgtatgtt cgcgtgtcgc tgcatgagtc gccggtgttt
     9061 gagaaagtcg ctaaagcgaa aaaacaggtg aagatcccgc tgggtacgct gctgaccaaa
     9121 catgttcgcg taaccgtact gggtacgttc attatgctgg caacctatac gctgttttac
     9181 atcatgacgg tctactctat gacctttagt accgccgccg cgccagttgg gcttggcctg
     9241 ccgcgtaacg aagtgttgtg gatgttgatg atggcagtta ttggttttgg cgtgatggtg
     9301 ccagtcgctg gattactggc tgatgccttt ggtcgccgta aaagcatggt aatcatcacc
     9361 acgctgatca tcctgttcgc gctgttcgcc tttaacccac tgctcggttc tggcaacccg
     9421 attctggttt ttgccttcct gctgctgggg ttaagtctga tgggtctgac cttcgggcca
     9481 atgggtgcgc tgttaccaga gctgtttccg acagaagtgc gttacaccgg agcatcgttc
     9541 tcttacaacg tagcgtcgat tctcggggct tccgttgcgc catatatcgc agcctggttg
     9601 cagactaact acgggctaag tgcggtgggg ttatatctgg cggcgatggc tggcttgacg
     9661 ttaatcgccc tgctgctgac ccatgagacg cgacatcagt cgttgtaata agagtgaaaa
     9721 gcctgatgcg ctacgcttat caggcctgaa tttccataca atatattgaa ttcgcaagga
     9781 tttgtaggcc ggataaggcg ttcacgccgc atccggcatg aacaaagcac actttgccaa
     9841 caatctaaat ctgtttaatt gccggatgtc agacatccgg caattatctt cacttcttca
     9901 tctgcgacaa aatcgtccgg cactgattag cctccccttc ggaaggtgag atcagtgcca
     9961 gcaacgctgc cgctggcgta accagcgttg ccagtgccgc agcaacagca ccacgggcaa
    10021 tcaacggccc ggctttcacc ccagcctgag gatttttaaa cgtcccacgc acatacagcg
    10081 gcgaacgcag tgtgataatc cgaatacctt tactctccgg atcaatagtc aaatccagct
    10141 gttccgaagc aaaacttgcc gtgccggtaa cgttaatcaa cgcgttctca gtatcgaaag
    10201 caaaaatctg cgggcgtgcc acgccgttgg caatattcag attcgccgcc gcgcagttca
    10261 cccgcacctc atcgtcacca aatatcgcac cgacaatgta gttgccgaca ttcagcccaa
    10321 caatctccat caggttgcgg ctcaccagcc cgtcattcat caacagtttc aggttgccgt
    10381 tactgttgcc taaaagtgcc gccaccgagt taccgctacc gcgcagttcc gcgtcaccgt
    10441 tcatttcccc cagcgtcttc tgcatcagtt ccacatcggg catcagttct ttcagtttca
    10501 gtcgacgagc ctgaatatct gcccgcccct gcatcggctt tttatcgcct tccagatgaa
    10561 tattcgccgc aatgctgcca cccgcaatgc caaatttcag cggttgcagg cgcaagtcag
    10621 catttttgag gatgatatga gtagaaagat cgctaatcgg caggctactg ccatgctcaa
    10681 tgcgccgccc tttgaagcga acatcggcat ccataacgtc ccatttatcg gtttcgaagc
    10741 ggtcataagg cagcactttg cccgcaggct gaacgctttt ttcgcccttc ttctgttcag
    10801 accgtttcga cttttctgcc cctttcccgg aatcaacgcc aatcaacggt cccaggtccg
    10861 ccagccgcaa ttgccgcgac tcgacatcac cttccagttt tggtcgtggc ttgccggtgg
    10921 tgtagaccag agaaccgtgg atatcgctat cgccaattcg cccattaaag ccgcgataat
    10981 caaagaccga cgatttttca gtgtcgattt tcgctaccag ccgaccatcc gtttcaaacg
    11041 gcggggtatc gggcagcaga acgcccgtca gttcatagag atcacccagt gaatcgccag
    11101 aaaatttaag ccgtaaatcg acaccgccca tcttcattgg gtcattcacg acgccatcaa
    11161 aagcaacacg ggtattacct gaacggaaat cagcctgtac cggaaacggc gtcccttcgc
    11221 cacgcagcgc cagcataccg cctatttttc ccgtacccgt gagcggttca ccgttataac
    11281 gtccctgcgc cttcaggcca aaaacgtaat cgcccacctt ttctttatcc gctttacctt
    11341 tcgatccagt aacttcgctg aacggcagcg gcttgcctaa gggatcaaca aaaatctcca
    11401 gatccgcttt gcttactttg tcatcaatgg cgatccgccc ttgatcgaaa agaatattat
    11461 ccagccgaaa cgaccatgcc gacggctttg cattcgcgtc tttgttatca tcgttggcga
    11521 gattaaacgt ccagttattg ttcttttcag agaggcgaat caggcgcgcg tcgggctttt
    11581 cgagcttgat ccacggcagc cagaccgttt tggtcagcag cgccagcggg gccagcgttg
    11641 cttctacgcg tggcaaatgc accatcgtga cttcgggaat atccggtgga ttgccaagaa
    11701 tgatgtcttc cgcgtgtaca tggggccacg gcacccagct gcgccagcca gtttcttgct
    11761 tttgccgctc ccacaccacg cccagatcgc cacggatagc gaacggacga ttcaactccg
    11821 cagagacttt ctggttgatg gtcggtttga gtcgattcca gtcaaaagtt gcaatcaaaa
    11881 tgatcgccac gacaatcaac aacaagaaag cccctgaaat cgcagcggtt attttgcctg
    11941 ccttgctcat cgttcgcttc ctcatattct tcctgtgcca gtcctaaaga tagtccagcc
    12001 aggcggaaaa tgaggcagct tatagcgcca gaaccgccgt attcagcgtt tctatcggtg
    12061 ccgggcgtga aagaaaccag ccttgtgcgg cgaatgcggg cgagttctgt acatcacgcc
    12121 actcttccgg cgtttctacg ccctcgacaa tcaccccgcg acaatagcga ttcattagat
    12181 gtaaaagctg agaaaagagt gtgcgtcctt ccggcgactg acgcagcatc acaaacagtt
    12241 ctcgcgcgat tttgatgtag tcataacgca cttcacttag tgcagagaaa tttgccatcc
    12301 cggtaccaaa atcatccagc cacagcgggc caaattcaca catcgaggca aaggttgaat
    12361 ctttcggcag acggatatgc tccaccagtt cgaaacgcag ccagggaaga cgctcaatct
    12421 ggcgcaggat ttttggttgc tgacgcaggg cgatgagcgt aggaccatca atattgaccg
    12481 atgccagcag gccgtgctct ataaagaagt cggctttttg cgccagcaaa tcaatctgct
    12541 ctttcacaac ctccatccga tggctgacgg tgatttcagt aaaatagcga tccggcggca
    12601 ggcgttgcga agggttcaag ggatgcgtga ccgccgttaa tagctccacg gccattaacc
    12661 gcccgcatgt ttgatagatc ggctgccagg tgtaagcacg ctcacactgc aaccaaaaac
    12721 gccgttcctg caagctctcg atgcttgctt cagggttgct tattcgctgg ataacctgcc
    12781 ttatcatcaa agatgtcctg tgtttaaaag tgatgcagcc cggactcgac aaagattatc
    12841 ggcgcgcaag ggcagaactt taccgcctgg ccggggtaaa tttccccact ccacaacgga
    12901 atctttcagg aatacgttct ggctcaaaaa aataatggaa cactgtttta atatggttga
    12961 ccagcaaacc accacagcgc aaactaacgc taatttttta cagatcaggt tcacgactat
    13021 gtccaaaaag attgccgtga ttggcgaatg catgattgag ctttccgaga aaggcgcgga
    13081 cgttaagcgc ggtttcggcg gcgataccct gaacacttcc gtctatatcg cccgtcaggt
    13141 cgatcctgcg gcattaaccg ttcattacgt aacggcgctg ggaacggaca gttttagcca
    13201 gcagatgctg gacgcctggc acggcgagaa cgttgatact tccctgaccc aacggatgga
    13261 aaaccgtctg ccgggccttt actacattga aaccgacagc accggcgaac gtacgttcta
    13321 ctactggcgt aacgaagccg ccgccaaatt ctggctggag agcgatcaat ccgcggcgat
    13381 ttgcgaagag ctggcgaatt tcgattatct ctacctgagc gggattagcc tggcgatctt
    13441 aagcccgacc agccgcgaaa agctgctttc cctgctgcgc gaatgccgcg ccaacggcgg
    13501 aaaagtgatt ttcgacaata actatcgtcc gcgcctgtgg gccagcaaag aagagacaca
    13561 gcaggtgtac caacaaatgc tggaatgcac ggatatcgcc ttcctgacgc tggacgatga
    13621 agacgcgctg tggggtcaac agccggtgga agacgtcatt gcgcgcaccc ataacgcggg
    13681 cgtgaaagaa gtggtggtga aacgcggggc ggattcttgc ctggtgtcca ttgctggcga
    13741 agggttagtg gatgttccgg cggtgaaact gccgaaagaa aaagtgatcg ataccaccgc
    13801 agctggcgac tctttcagtg ccggttatct ggcggtacgt ttgacgggcg gcagtgcgga
    13861 aaacgcagcg aaacgcgggc acctgaccgc aagtaccgtt attcagtatc gcggcgcgat
    13921 tatcccgcgt gaggcgatgc cagcgtaaac gcagcatatg caaaaggctg gataaagagt
    13981 tacccctcta tccagctcat ccataaatac ctgatggttg tgctaactca tcaccatcag
    14041 gcattactgt gcaggtggaa tatccgtcac ttcaggatgt acgtcatccg tggcgacaga
    14101 ggtggtcgca gcggcggtag atggggccat gatttgatcc caggccgcct gcaacgcttt
    14161 catgttaaat tccggctcgc ctttcggctg cagcagtatt aacgccatat cattcgacaa
    14221 ctgctgacgc agatcctgat ttaacatctc cacagtcagg ctgttaagga aatcctgccg
    14281 cagtttctga tactgctccg gcgcgatatc gacgacctga ttttgcaacg aacgcatccg
    14341 ctggcccatc agaatatcgg tatcagcccg tgcataggcg gcaaacagtt tctgcagctc
    14401 cagtttcttt tgcgccacta acgcattgaa ctcttcttcc ggcagacctt tatcgcgaac
    14461 cttcgccagt tcacgcgcca ccagattaag gttgctgttc agcttgtcgt ttggtgattc
    14521 gatgttgatg gcacactgcg cacgcagata cagcacacgg cagtcaaatc caagaccgat
    14581 gtctttgctg ttactggcgc ttaacgcttg ctgaacatgc cagaacagcg cctcacgggc
    14641 caggtccgca cgccagtagc gcagcagtgc ggctgattca cgaatcggct gccacggcgt
    14701 atcccacatg atagataacc ggtcctgacg caccgcgtca gtcataatac tcaccgcttc
    14761 cgcacgcagc ggagaaagcg tcggcaccgg agccggtgtt tcacgtttgc ctttcagttc
    14821 gccaaacgtt ttgttgattt ggtcgacgac cgagcgcgca tccacgtttc ccaccaccag
    14881 cagcgtcatt gcatccgggg tgtaccattt ctgatagaaa tctttaattt tttccgcttc
    14941 aacgggttgt ttcagcggat cggcaggatc gtgacctaac aaggttgacc ctttcagacg
    15001 atagcgccac cagccctctt tagtatcggc aggccaggtt gccaccatgt cctgactttg
    15061 cagcgcgtgg ttgatggttt ctggcgtgat agtcagtttg ccagtggcat ttgccagata
    15121 agagagcgcc tctttcagca aatcgttacg gttattgggc aaactcagat taaacagcgt
    15181 ggtgtcataa gagacaatta ccggcggcat cgggcgttta gggtcgatcc cctgctgcca
    15241 caatgaacgc gcctgtgctg cgtcaaggcc accgctttgc gttagcgcaa tacgagggat
    15301 ggcgtgactg taaccgctct gttgtgtact ttcggcgagc gaaccggtat taaccagcag
    15361 gcgaatttca acacgatcgc tgggacgctg gggggtggtc agcacttgcc actgtaaacc
    15421 gttggaaagc gtcccctgtt gccatgctgg atcaggctgg agcgcatctg cctgcacata
    15481 gccagcagtg gccatcatca gcaaaccgcc cgctaaaagt cgaatttttg tgccctgcat
    15541 gtgaacccct gatcaactat cctggtaata aaaaaactgc ccgttgtcgg tcagtctaaa
    15601 tgacgttaaa aacacttcgt gttagaccgc aagaacatga aaatgtcacg gaagaagtga
    15661 aataaacctg aactcaccca ggtggtaagt tcaggcacag gggtcaatta tgcgcaaaca
    15721 cccgcactcg gggaagggag tgcgggcata agtgatgaga ttaagaggat aattcgtgcg
    15781 ttttgccatc cggcgcacga ttattcagcg catcgtccag ttttttgtgg tccagttctt
    15841 tcacccactt agcaacgaca atggtcgcta cgccgttacc gaccaggtta gtcagcgcac
    15901 gagcttctga cataaagcgg tcgataccga ggatcagcgc cagacccgct accggcaaat
    15961 ggcccaccgc agagagcgtc gccgccagca cgataaagcc actacccgtt acccctgccg
    16021 cccctttaga agaaagcagc aacacgatta acagcgtgat ttggtggacg atatccatct
    16081 gactgttagt ggcctgggcg ataaacaccg ccgccattgt caggtatatc gatgtgccat
    16141 caaggttaaa cgagtagcct gtcgggatga ccagccccac caccgattta cggcagccga
    16201 gtttctccat cttgtcgagc atacgcggca gcgccgactc ggaagatgaa gtccccagta
    16261 caatcagcag ttcttcacgg atgtagcgga taaatttgaa gatactgaaa ccagtcgctt
    16321 tagcgattga acccaatacc agcaccacaa acaggataca ggtaatgtag aaacagataa
    16381 tcagctgccc cagttgcacc agtgtgccga cgccgtattt accgatggta aacgccattg
    16441 ccccgaacgc accaataggt gccagacgca tgatcatatt gatgatgccg aagatgacct
    16501 gcgagaaact ttcgatgacg ttaaaaatca gttggccttt gctgcccaga cggtggagcg
    16561 caaaaccaaa cagtacggca aacagcagca cctgcagaat gttaccgctg gcaaatgcgc
    16621 caatgacgct cgccgggatg acatccataa tgaaggcgac aatgccctgg tctttcgcct
    16681 gatcggcgta aaccgctacc gctttcgcat caagcgttgc cggatcgacg ttcattccgg
    16741 caccaggctg cacgacgtta acgatgataa gaccaataat cagcgcgatg gtactgacaa
    16801 tttcaaagta aagcagtgcg actgcgccgg tacgaccgac cgccttcatg ctttccatgc
    16861 ccgcaatgcc cgttacgacg gtacaaaaga tgacaggagc gatgatcatc ttaatgagct
    16921 taacgaagcc gtcgccaagc ggtttcattt gctcgcctat ttcaggatag aaatggccaa
    16981 ggagaatacc aatggctatc gctgtcagga cctgaaagta aaggctttta aacagagagg
    17041 ttttcatagg gtgtccttta gtaaaaccac aggtcttgta aggttatggg gtacctgcgg
    17101 ccttaaaata acacccagac aacatcacag aaatgtacct ggatcataat tgaaacaaaa
    17161 aggttaaaaa gtttgagctg gctcgcacaa accagcactt tttaaagttt tgtaatcagt
    17221 ttggggtagc tacttttctt ccaggtaact ctcttcgaag atttcaatag ggagtggacg
    17281 agcaaaaagg aagccctggg caataccaac gcccgctttt gccagccagt cgcgttgtgc
    17341 ttcagtctcc acgccttcgg caatcatttg taagttcagg ctctgcgcca gcatgataat
    17401 tgcagcaatc atgctgctat cttccggcaa gccttcaaca aacattttgt cgattttcag
    17461 aacgtcgatt ggcaacgatt tcatatgctg cagctgacgc agccctgcgt agcccatgcc
    17521 gaaatcatcc agcgccaccc gaactccggc attacgcagc ggacggagga ttgccaccgc
    17581 agcatgaggg tcgtcaatac gtcggctttc tgtcacttcc agaatcagtg ttcccggctg
    17641 aatgcgatag cgggttaaca gttccagcat atccgccacc atattcgggt gcatcagttg
    17701 cagcacagag aggtttaccg acaagggcag cataatgccg cgctcttgcc aggctgcaag
    17761 caatcgacag gactcttcca gcacccagtg accgacggta accatcagcc cacagcactc
    17821 aatgcgatcg attaagccat ccggcaggtc ccaactgcca tccggttgct ggatacgcag
    17881 taacacttcc gcactgacca gtttaccgct ggtcatctcg acctgtggct gtaaccaaat
    17941 agcaaactga tgattttcca gtgcattaag gatatcgctc tcttccgtca accgcttctg
    18001 ggcggcttcc atctgctgcg gatcaaagaa ctgaatctga ttcttgcctt tatggcgagc
    18061 ggtaaatgcc gcagaaatag cgcgactgta aagctgttcg gcggtgagat cgccgtagaa
    18121 catcgccacg ctaatgctac agtgcggacg gagttgaata cgttcaatcg gcaggcgctc
    18181 gctcatgata gtgagcactt gctgacccaa agtgattgcg tgccacggtt catgtacacc
    18241 gttggcaatg acagcaaagt cataaccgct aatctgcgcg aggatcatac gtggcgacag
    18301 taccgatttg agtttttcca ccagcgtcag cagcagaatt tctcgttgcg cctctttcag
    18361 cacgcccgca gtatcacgca gagtttcaca ggtgatgatc atcagcgcgg tggtttgttt
    18421 acgcgcgaca acctgctcca gcatctccat cagcaaggct ttgttcggca aatccgacac
    18481 cgggaagcgc atcgcatttt cgttctgttc ttcataatgg cgctgcagca attgctggtt
    18541 gaggttgtaa ctgcgcacca acataccgat ttcatcgtcc tgatgcagac gcggtaatgc
    18601 cagttggtga ccaacaagct cctgggctgg gatggcgtta agttcgcgag caatattgcg
    18661 taacggatgc aaaatcaggc ggttaatgca ccagctaatc gccaccgtca atattaacga
    18721 caaaagtaag taaatggtca ctaacgttga gagggtgctc atcacgaact tatacatacg
    18781 gaaggaatcc gcctgtagca ccagataggc aattggctgc ggatttgccg gacgttcgag
    18841 cgagtagacg cccagcgaga tttgaaccgg tagctcaaac aggcgagtaa ccattaccgg
    18901 caccggacgc tctggaataa aacttttacg cagcgcctgg aactggttag gcagcactac
    18961 atcggcacgg ctgaccacgc ctgccggttt aatgctggca agaatagctt ccgcttcggg
    19021 aatatcacct ttcaaaatgg ctgaagataa aggttcgcgg acagagcgag caatgctttc
    19081 cagttgcgta gccgtgttat agcgattctg ctggaccaga tggaacagca aaacggtgca
    19141 aaaaataaaa acgaacacca ggacaacggc tgccaccatt gccatctgct tgattgttaa
    19201 cgagcgactt acgcgcaaat tgtctccccc cggaaatccc agattgccgc ccgagtatac
    19261 ccgatcgcag cggcattaag agaggcgcta tctgaaaact taccagtcgg cgtaaggtat
    19321 cagcggctgc ggcggtaaat ccatgtcacc ctgccatccg gcggcggaat aacgtacgta
    19381 gagcaggaaa tggctggggg cgtaatcttt cgcctgctgg atatcaattg ccgtgccgac
    19441 aaaccagttg gaagtaacac gtcgttcaag taatgcccgc gccgtatagc cgaagccctg
    19501 actgctgccg ccatcgttgg attgccgcgc agcttcttcc tgccagtcgg tcgggatcag
    19561 attcatcagc ggataacgcg gcatggtttt ggtgcgtgaa tgcgaccacg agccagacgc
    19621 ccccagctcc cacgaccagt tttccgtgcg ctcccgccac atcaccggta tggcaaacga
    19681 caggtattcc tgcgggctgt agtaaccgcc ctgaccgagt gagtagccac tcaggtcttt
    19741 gtcgtaatgc cagatcatgt tgttcaggcc gattgtgacg cggcgattgt tctggttgat
    19801 gaccttatag taatagcccg tcatccagcg cacacgccag ttatcttcga catttttacc
    19861 ggttaactgg tcgccactaa gcgatgccca gacgccgttt gcttcacctt tatcgtagct
    19921 caggcttagc cccacgccgt cggcacgcac gccgccccat tttttcccgg tattgctcgg
    19981 ggagtctttt tgcccaccaa aggccagcaa agaactggag atgggacgac ggtgggcgtt
    20041 aacggtgtaa cccagcggcc cgatatcatc gctgtaactg atgccgccga ccacatccac
    20101 cacgttgaag cccatcggcg tggtgccgat atcccagctc cagacgtcat ttcgccagcc
    20161 gaccgccacg ctggcaccgg aatccgactg gctgcggttg ccgctacagt cccgtaatgt
    20221 acaggtgccc cagttgtcat cccatttgcc atcggcatta gtggagaaac tgccgacgtt
    20281 catattgacg aaatcactgc gaaagaacat ccgcccgtca gaatacggcg catccacctg
    20341 caacatggta gtgtgcgctt tcagatcgga gtaaccaccg gtgccgctcg aaccccaata
    20401 atcatgctcg agggtgacgt taagatcctg ctggcgatag aggtccgccg catcgctgcg
    20461 cacgccgcgt ttcagccagt catctttctc gtcgttacgg gtcagtcggg taaaggtgtc
    20521 gttatcctgc ggacgcgtcg tagtcacacc ggatgcgacc atggcgtctt tgtaggtttc
    20581 cagcgcctgc tttggatcgc ccgcctgcgc ttcaaacttc gcaccgtcgc gcagcaccat
    20641 cgcgctttcc atcgacggtg gctgagattt tgcctgcggg atcaacttat taaacgtctg
    20701 ctgcgccgct gcggtatctc caagctgcgc ctgcgccagc gccacgcgcc gctgtgtgtt
    20761 cagcgaggcg ttatcagtag cgggcagttt cgccagctgg ctacgtgccg ccgctgtgtc
    20821 accggcagca atatccactt ccgtcagacc aagaatggca tcggcgttag ttggctcccg
    20881 cgtcaggaca ttctgatatg cagcgcgggc ggcggtgtaa tcacgtcgct gcagcgccca
    20941 gtcagccagc gtgaggtcaa tacgcgtgga aggtggttgc tgacgcagca tcgcttccgc
    21001 ttcagcttct ttgccgcttt ctcgcaggcg attagcggtt tccagcacct gatcgctttg
    21061 cagtcgatta accagctcct gaatattgct gctccactgc gcacgcggca ggctgttgat
    21121 atgcgccagc gccgctctgt cctggtcatg accagagagg tacagcccgt aagcgtaaac
    21181 ctgctccggg tcgttcggct tctgctgcgc cagattgcgc attaacgtat cggcctggct
    21241 gcgttgtccg gcctgccaga gatcctgcga aagtcggtaa gtaatccata cgctgccggg
    21301 gtccagcgcc agtcgttgcc gctgaagtgc tgccgcctgc gcccatttgc cctggttttc
    21361 cagtgcctct gcctgctgtg ccagacggtc gttttgcagg ctgcgttcga tatcatcaat
    21421 gctacgccgc tgactggcag agagtgaggc gataaacgct tcagcttttt ctggcgattg
    21481 ctggcggtaa atatttgcca gcccgcgcac ggcgttagtg ttgccgctgt ccatacgcaa
    21541 ggtctgctga taataacgtt cggcggcggg ataatctttt cgcgccatcg ccacatcgcc
    21601 cagccccagc actgcataac tgtcggtgtt atcgacatta cgcgcctgct ggaacaggcg
    21661 ttctgcccgg tcaggattat tggctttcag cgcagcatcg ccctgctgga tcgccagcca
    21721 gtagcggttt actttcagca gactgttcca tttgtcgttg ttgctgctgt gcggatccag
    21781 tgcgagggct ttttccagat tcgccactgc attggcgcga tcgcctttct gtgaatatgc
    21841 ctggcccagc gcccccagag cttcactgtc tttcgggttt gcccgcactg cctgttgtag
    21901 ttcgggaatg gctttacccg ccataccaga gtccaccgcc gctaaacctt gcgcacgagc
    21961 gcggaaagca ggatcggcca gctgtttttg ctgttctgcc agttgcgatt gcgcagccgc
    22021 cacgctatcg ccatcactaa agatagaaag atattttttc agcgccgaca cactggcatc
    22081 actgacgggc atgtctttaa tctgcccgta ccagatttta gaggcccctt cgcgcccggc
    22141 gttcgatttc gccatctgtt ccaggacggc aaaaccttcg tcacggcgat cgctactaaa
    22201 cagcaatagc gccagattgt tttgcaggcc cgtattgccc ggtgcatccg cattgatgcg
    22261 ttttaactga ttaatcgctt cgccacggcg agccggaatt ttcgccaccg tactccagta
    22321 ctcgacagca atgtcacctt ccggcggcgc accgttgaac agtttgttgt aactcgccac
    22381 agcttcttcc gcatgaccgg tagtcgcctg caatcttgcc tgttgcagtg cctgacgacc
    22441 atccggcgtg gaaagtagca tcgtagtccg cgacgattta tacgcatttg aactcggcgc
    22501 taactgcgac agccgatcga gctgtttttg cgcgccatca atatcgccct gacgtaacaa
    22561 agaacggaaa cgggcggcaa cgacgtccgg gttattcgga tcaataagtt ccagccgata
    22621 caacgactgc tgcaccaggt cttcacgatg ggtcgcttcg cctaaccgaa cttgctccag
    22681 caactgttgc tgagcggttg gtgctgcctc gaccatcggc atgacggcca gaccgaggga
    22741 aagcgtgaat atgtttagtg tgaatttgcg cattcctggc cccagtcagg taataactca
    22801 ccttttgtcg agaagcggaa acggtgttga tcccagcctt gtccaaacag ggtcagcaca
    22861 tagttgtaat aggcatcgct gccgggaaag ttatcggcca cgcgctggcg ctgaacggcc
    22921 tgcgcatcac ggttttgtaa aaagggcagc atggcggcag aaaaaccgac cggtccttta
    22981 ccctgcgctt tccccgtagc cacatccact ttttccggcg gataaccgtt tttctcagtg
    23041 aatgtcgcca tcggtttaaa ccggttgagc atccgcgctt tctgcggatc gctgtcaggc
    23101 atcatgccta cccacatgta aacgcggata gcgtcgtagc tgctgatcaa tgttttttcg
    23161 gcttttagct gccagccttt gtctttctca tagcgcaccc agtctggcga aaagcctttc
    23221 ggggcggttt ccagcaataa acgttgattg gtttcgcgca gcgtagtcca cggcgcgcca
    23281 aagcgggtga aatactgcgc cagcgtcggc ggcaggtagc tggggttaaa acgccagctg
    23341 ttatcctcag caaaacccac tttgcccggt aacaacatgg aacccagccc aggcaccgtc
    23401 accacttcct cccgcgcaat acgttttagc aacgcgctgc cgatgtcggt ataacgctgc
    23461 tctttccaca aacgccccgc ctccagcaac gaccaagcca tccagacatc accatcggag
    23521 gccgaattgc tgtccagcac ttcccactta ctgttctctt tcttgcccca cagccaggcg
    23581 ggcaaacgtt cttttaaaga accctgagcg agattgttct gcgtccagtc gagaatatta
    23641 tcgaaagctg cacggtcgtt agccgccagg gcaaagaaca tgccgtaact ttgcccttca
    23701 gaggtggtga ttttgcgcgc gtcgctgggg tcgatgacgc gcccttcctg actgatgtaa
    23761 tcctttttaa actgctccca ggcaggccag gtacaggctg cctgaacact aaaggcagcc
    23821 aacagcagca tcgtcacgag tccactacgc aacacattca tcttcaatta ctcgttatcc
    23881 gggttaagac gacgacggct gataatacgc agcaaacgcc acagtaccca tgccagcaat
    23941 atcacactga tagccgccag caccgccagc accgccagca ccgccagcac cgccagcacc
    24001 gccagcagaa tcggatggtt tgccagcgca taccacaagc gctcgaacca cggcagatga
    24061 cctacgtaat aaacgtcgcc aacacgtagg ctgttgatac cggactcgcg gatcaccgcg
    24121 accgaaccga acatggtggc gcgtttgccg ctatcgttca ccgcatcgtt aagcatttca
    24181 taaccgcgtg ggctatctgc cagcagcgca atcacgctgc gctggtcgtt atacggcgac
    24241 tggaagccaa tcaccgccgc catcgcaccg gaagaggtca gcgttgactg ggtttctgcc
    24301 gcgcgatcgc tctcgtccgg cacaatgccg gggaacgggg tctggcgcat cggtgttttc
    24361 acccagcttt cggtcgcctg caccaatagg tcgatctgct tatcgtcttt cagtttgtcc
    24421 gggataccac cgatgatcat gatgtcggca tctttgccct gaatggtgct gccatcatcg
    24481 gtcaccgtca gattaatcgc cgggaagccc gtctgtgcgc cgataaaacc aacagtattc
    24541 agcaacgttt ccatctgtgc ttcgttaggc gttttcggca tcacggtgat ggtttgcgac
    24601 agatccgcca tccggctgaa cgggaagccc gcgttagcaa aggcgcgtag atccggcatc
    24661 gggatgaagt ggtaatactt cgagaagtcg atggtggagt cgtcaccaat caccacatga
    24721 ttctgcaccg gctggaaggt aatacagtta tccaccgaac cgccaggcat cgggttcata
    24781 tactcaaagt cgaagcgcag ctggttggtc gcgcccagtt tcagcgccgg aatagagaca
    24841 tccgttttgc catccagcaa accttgtaat accggaatcc gcagcagcag gcggttcgcc
    24901 tcctgtttgc tgctcaggtt gaaggattgc aggaactggt tattcaggct gatatccatc
    24961 cgcgaactgt ctttcaccgg cggcatggtg tagcggtaat taatatccat atcaatgccg
    25021 gtactgcgca tcaggtagag atctggcggc aggtttagcg aaacattaat cgctgctggc
    25081 tcaagaccgc tggattgtaa ctgttcttca taggttttca gttcaccaaa ggtgaccgga
    25141 cgatcggtac gcacccagtt cggcgcatcg tacggcttac gcggtagcag cggtttcact
    25201 tcattcacta ccacgctttc accacggaac agaatgttac cctgagcgat acctttcgct
    25261 gcctgcaaca ggtctttgtc gtcacgacca aacaccacca gcagtttgac gtaaggattc
    25321 tgcggatggt taatcatctc aatcaccggg gcttttaccg ccggatgatc gcgcaggaag
    25381 tccggccgtt tgtcgttggt tgcaaagaca atggcattac gatccggcag ttggttatag
    25441 agcaccggga agttctgccc acgccagcca gaacgcgaac caaaccacga ggcgacaatg
    25501 gcagatgctt gttgcagccc aacatccggc gcacccgcaa agaccatcgg caaggtgttg
    25561 gtgcggttat cacgcgggtc aaagaatggc accgggaagt gtgacaggtc attcttcaca
    25621 ttcagggtct gataggtcag atccagtcca ctgctccgcc caacatccag ccaaagcgtg
    25681 gtgctggccg ggttttcgca cacgtcctga taatggccga caaactccag ccgcacacgg
    25741 ttgaagtcgg taataaacag tggattaatg ggcatttgcg ccagcgtttt tttacccaac
    25801 tgttctttgg tcactggcag cacgcccatc agctcatcat tgagataaac ctttaactgc
    25861 gactggacag gcagtaacga tggcgatggc gtgtattcga ggttgagcat cgccttcgtc
    25921 accacttcat cgctgcgcat accaaactca atgctgccgt tcgggttaat gccacgtagc
    25981 accatgctgc ccggcggcgg cgcaatttgc gcaaaggtca gcttcacatc acgcgaagga
    26041 ccgttctgcg ccacgactgg cgcatcagcg ccctgcacgc ccggcatcac ttgccccacc
    26101 tgcggatttt gttccgtctg ggcggctaca gctggctcag cattgatcag tggttgcgtt
    26161 gctggcgtcg cctgcgtcat gaaagagggg aacgcactca tccccatagc cactgcacaa
    26221 atccagaata gttttctttt catcgcgtta tcatcattgt tgagccaaag cctgatccga
    26281 tggttgtgcc gtttcgctcc gctccgggcg gcgcggaata aacgatacaa cccaggaaac
    26341 cagagaagtc agcacacgga atatgccctt caccgaagaa ggcgcaaact ccgccagatg
    26401 gcggtagcca cggaagccga gcttcagaat atccagcaga ctttccagcg gcttatcttc
    26461 cgggtagctg tcctgccaga gcgcccatgt atccgcacgg gcaaacgtac actgcacaaa
    26521 atcgatatgt tgctgggtgg tgagcggcat taatttcagc ccaacttcat tacccatcac
    26581 gcgcgccacc tgggtcggga agacgtattc ctgctgaccg cgtttaagca acagattcac
    26641 tttctgccct tccagaatct gcgcctgacc gttgatcttg atccccaaac caccgtcgga
    26701 gaaatcctga acggtacacg agaagaggtg accatcttcg cgggcaattg ccgcgggcat
    26761 cgtcatctcc acgcggtgcg atcggcgtac ctgtttgctt tctaccgata ccgcaactgc
    26821 gccgccaaga acaatcaggt tgtagaatac ccacaccata ctgacgacca cggtgagcat
    26881 ctcggttggc gggccataga agtagcgcca gatgcctacc gcaacgccca ccaggttgag
    26941 cagaacaagg aagatgtagg gccgcgagat cacccagtcg acgtactctt cttccaccag
    27001 tccacctttg gcggtgacgt taaatttgcc tttgtgcggg ttaatcagcg ccaccagcgt
    27061 cggtggtgcg atataccacg ccagcaccgt ttcgtagatt tcactccaga aagagtggcg
    27121 atatttgccc tggatcttgg agttggtcag gctggcatgg atcatatgcg gcagcacgaa
    27181 cagggcgatc atcaacgctg gcgcatagat gatgtaggca tgaagcagca ggaacgccag
    27241 cggcgcagtc aggaagatca gccgtggaat gcccgacaag aagtggaaca tggcgttgac
    27301 gtagcacagc cgctgagcaa acttcagccc tttaccggtg agcgggttat cgagacggaa
    27361 gatttgcacc atcccgcgcg cccagcgaat acgctgaccg atatgcgccg acagactttc
    27421 ggtcgccagc cccgccgcct gcggaatacg catatacgcg gaggtatagc cacgacggtg
    27481 caaacgcaga gaagtatgcg catcttcagt cacagtttcg acagcaatgc cgccaatttc
    27541 atccagcggc ttacggcgaa tcaccgcaca ggaaccgcag aagaaagtgg cgtcccacat
    27601 atcgttgcca tcctgcacca gaccatagaa cagcgtgcct tcgttcggtg ttttacgaaa
    27661 acgccccagg ttgcgttcaa acgggtccgg cgagaagaaa tggtgtgggg tctgcatcat
    27721 cgccagctgt ttctctttca ggaaccagcc cacggtcatt tgcaggaacg atcgcgttgg
    27781 tacgtggtcg cagtcgaaaa tcgacacgaa ctcgcctttg gcatatttca gcgcattgtt
    27841 gatgttgccc gctttcgcat gttcatgagt ggtacgggcg atatacttca cccccacgtt
    27901 ttgcgcaaac tggcgaaact cttccctgcc gccatcatcg aggatccaga tgttcagctt
    27961 gtctttcggc cagtcgatac ccagcgaggc gtaaatggta tttttcacca cgttgagatc
    28021 ttcgttgtaa gtcgggacaa agatatccac cgacggccac agcgacatat ctttcggcaa
    28081 tggcaccggc tgacgattca gcggccatac tacctggaag tagccgagca ccagcacaat
    28141 ccacgcgtac gtttcagcga agagcagaat aagcccgcac accaggctga ccggatcgtc
    28201 ccagttcagc gtagaggtat atcgccacca gatataacgg caagaaacag tcagcgacag
    28261 cacaatcaac attagcgctg agaagcgccc cggcatccgc cgtacgatca gcgctccccc
    28321 ccacagcagc atcaggaaga taaactgcgc cagcgggtta aacggctgag taacgcagat
    28381 taacgccaga atcagcgaga aggtgacgat gataccgagg atcaaccgcc gcgccccggc
    28441 actcaaatga ccgagctctt ttttctcatc aagatgctgt gttttatggc taacgcgctc
    28501 aggcagctcg ttcatccatt gatggtaacg tccacgaata ttctgcagac ctgaaaatgc
    28561 ccgcctgcgc ggtttcggcg tttctttgcg cgatgcaccg atcagtaacc agcatgtttg
    28621 aatgagataa cggaccgggt ccagcggacg cggacgcgag gcgttgatat gcggatacag
    28681 gtttttatgt tctgcgcgaa tacgctgcca gcgcgggtgc tccagtggaa taaaaatcca
    28741 ggccaggatc atccagaaac agccgagcgt cgcgctgaaa gccgacgcac cgtgacgacg
    28801 ataatcgcga taacgcccga taagccgcgc gttgaccggc gggataagca accaccgggt
    28861 caggatactc atgatgcact cccgactggc gttttcagcc cggaatagtt caacaggcac
    28921 cagttcgcca gcgtcaatat ctcttcagcc gccagcgcat cactgcgata ttcacctact
    28981 ggttgcttag ccgccaggca ttcagccatc gcttcatcac gatgaatgag catcggcagt
    29041 aatcggcgct ggctttgcaa ccaaagctgg taaatatcgt cctgaacctg actgccaata
    29101 cggaagttat taatcaaaat atgtgcgcca tccggcagcg cttgctgatg cagtcggata
    29161 tggcagttgg catccacatt gacgattgcc agcgagtgat cgcacaaact cagcagctgg
    29221 tgggttatct gcgaggcatc acgcggtaag tcgattaaaa tccactggta acgcccgctg
    29281 gcttttagtt gctgtaagcc ggagcaaata tcgctcagcc gggtttgcca gtgctgtgga
    29341 ttttcttgtt cttcaatgga taactgacca aaaggcagca aatcgagctg cgaggtgtag
    29401 cgcaacccag cgtcgcgcca gtcctagcca tccagcatag ctctggccca gccctgacgg
    29461 tgggtaaaat caacattaaa tgacaggcgc aacaagttgt ccgggcaggc atcgaccacc
    29521 aggacatttt
//
LOCUS       DBFIFM010000040        28501 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.040, whole genome shotgun sequence.
ACCESSION   DBFIFM010000040 DBFIFM010000000
VERSION     DBFIFM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.040"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..607)
                     /locus_tag="N7C74_001538"
     CDS             complement(<1..607)
                     /locus_tag="N7C74_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGU2431433.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPE"
     gene            complement(678..2792)
                     /gene="fusA"
                     /locus_tag="N7C74_001539"
     CDS             complement(678..2792)
                     /gene="fusA"
                     /locus_tag="N7C74_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HGU2431434.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2889..3359)
                     /gene="rpsG"
                     /locus_tag="N7C74_001540"
     CDS             complement(2889..3359)
                     /gene="rpsG"
                     /locus_tag="N7C74_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HGU2431435.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3456..3830)
                     /gene="rpsL"
                     /locus_tag="N7C74_001541"
     CDS             complement(3456..3830)
                     /gene="rpsL"
                     /locus_tag="N7C74_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HGU2431436.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3956..4243)
                     /gene="tusB"
                     /locus_tag="N7C74_001542"
     CDS             complement(3956..4243)
                     /gene="tusB"
                     /locus_tag="N7C74_001542"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HGU2431437.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSGQMAW"
     gene            complement(4251..4610)
                     /gene="tusC"
                     /locus_tag="N7C74_001543"
     CDS             complement(4251..4610)
                     /gene="tusC"
                     /locus_tag="N7C74_001543"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HGU2431438.1"
                     /translation="MKRIAFVFSTVPHGTAAGREGLDALLATSALTDELAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLDLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4610..4996)
                     /gene="tusD"
                     /locus_tag="N7C74_001544"
     CDS             complement(4610..4996)
                     /gene="tusD"
                     /locus_tag="N7C74_001544"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HGU2431439.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANHLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDEMEAGRLGLASSN
                     LQQGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4996..5718)
                     /gene="yheO"
                     /locus_tag="N7C74_001545"
     CDS             complement(4996..5718)
                     /gene="yheO"
                     /locus_tag="N7C74_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU2431440.1"
                     /translation="MSRSLLTNETSELDLLDQRPFEQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5885..6697)
                     /gene="fkpA"
                     /locus_tag="N7C74_001546"
     CDS             complement(5885..6697)
                     /gene="fkpA"
                     /locus_tag="N7C74_001546"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU2431441.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6918..7136
                     /gene="slyX"
                     /locus_tag="N7C74_001547"
     CDS             6918..7136
                     /gene="slyX"
                     /locus_tag="N7C74_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HGU2431442.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7185..7775)
                     /gene="slyD"
                     /locus_tag="N7C74_001548"
     CDS             complement(7185..7775)
                     /gene="slyD"
                     /locus_tag="N7C74_001548"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGU2431443.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7870..8070)
                     /gene="yheV"
                     /locus_tag="N7C74_001549"
     CDS             complement(7870..8070)
                     /gene="yheV"
                     /locus_tag="N7C74_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HGU2431444.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8107..9885)
                     /gene="kefB"
                     /locus_tag="N7C74_001550"
     CDS             complement(8107..9885)
                     /gene="kefB"
                     /locus_tag="N7C74_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HGU2431445.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERR"
     gene            complement(9885..10436)
                     /gene="kefG"
                     /locus_tag="N7C74_001551"
     CDS             complement(9885..10436)
                     /gene="kefG"
                     /locus_tag="N7C74_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HGU2431446.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10567..12480
                     /gene="yheS"
                     /locus_tag="N7C74_001552"
     CDS             10567..12480
                     /gene="yheS"
                     /locus_tag="N7C74_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2431447.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12480..13502
                     /gene="yheT"
                     /locus_tag="N7C74_001553"
     CDS             12480..13502
                     /gene="yheT"
                     /locus_tag="N7C74_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGU2431448.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13496..13714
                     /gene="yheU"
                     /locus_tag="N7C74_001554"
     CDS             13496..13714
                     /gene="yheU"
                     /locus_tag="N7C74_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HGU2431449.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13768..14637
                     /gene="prkB"
                     /locus_tag="N7C74_001555"
     CDS             13768..14637
                     /gene="prkB"
                     /locus_tag="N7C74_001555"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HGU2431450.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAIELIML
                     PLVQRLMEGKKIE"
     gene            complement(14692..15096)
                     /gene="yhfA"
                     /locus_tag="N7C74_001556"
     CDS             complement(14692..15096)
                     /gene="yhfA"
                     /locus_tag="N7C74_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HGU2431451.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15398..16030
                     /gene="crp"
                     /locus_tag="N7C74_001557"
     CDS             15398..16030
                     /gene="crp"
                     /locus_tag="N7C74_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HGU2431452.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16081..18171
                     /gene="yhfK"
                     /locus_tag="N7C74_001558"
     CDS             16081..18171
                     /gene="yhfK"
                     /locus_tag="N7C74_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HGU2431453.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18238..19458)
                     /gene="argD"
                     /locus_tag="N7C74_001559"
                     /gene_synonym="dapC"
     CDS             complement(18238..19458)
                     /gene="argD"
                     /locus_tag="N7C74_001559"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HGU2431454.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPSDIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGQARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19544..20107)
                     /gene="pabA"
                     /locus_tag="N7C74_001560"
     CDS             complement(19544..20107)
                     /gene="pabA"
                     /locus_tag="N7C74_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HGU2431455.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKVVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20139..20741)
                     /gene="fic"
                     /locus_tag="N7C74_001561"
     CDS             complement(20139..20741)
                     /gene="fic"
                     /locus_tag="N7C74_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HGU2431456.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKTKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20731..20898)
                     /gene="yhfG"
                     /locus_tag="N7C74_001562"
     CDS             complement(20731..20898)
                     /gene="yhfG"
                     /locus_tag="N7C74_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HGU2431457.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRSHYER"
     gene            complement(21003..21575)
                     /gene="ppiA"
                     /locus_tag="N7C74_001563"
                     /gene_synonym="rotA"
     CDS             complement(21003..21575)
                     /gene="ppiA"
                     /locus_tag="N7C74_001563"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HGU2431458.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21846..23027
                     /gene="tsgA"
                     /locus_tag="N7C74_001564"
     CDS             21846..23027
                     /gene="tsgA"
                     /locus_tag="N7C74_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HGU2431459.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVAKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23289..25832
                     /gene="nirB"
                     /locus_tag="N7C74_001565"
     CDS             23289..25832
                     /gene="nirB"
                     /locus_tag="N7C74_001565"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HGU2431460.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25829..26155
                     /gene="nirD"
                     /locus_tag="N7C74_001566"
     CDS             25829..26155
                     /gene="nirD"
                     /locus_tag="N7C74_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HGU2431461.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26281..27087
                     /gene="nirC"
                     /locus_tag="N7C74_001567"
     CDS             26281..27087
                     /gene="nirC"
                     /locus_tag="N7C74_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HGU2431462.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            27106..28479
                     /gene="cysG"
                     /locus_tag="N7C74_001568"
     CDS             27106..28479
                     /gene="cysG"
                     /locus_tag="N7C74_001568"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HGU2431463.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6871 a         7672 c         7226 g         6732 t
ORIGIN      
        1 gttccggaat gtaggaatcc aggaagccag ccagttccag gattttcgct tcccactctg
       61 cgtcgccttc cagcgctttc agagcagaac cacgaacgat cggagtgtcg tcgcccggga
      121 agtcgtactg agacagaagt tcacgaactt ccatttcaac cagttccagc agctcttcgt
      181 catcaaccat gtcgcatttg ttcaggaaca cgatgatgta cggaacgcct acctgacgac
      241 ccagcaggat gtgctcacga gtctgcggca tcgggccgtc agtcgcagca actaccagga
      301 tcgcgccgtc catctgagca gcaccggtga tcatgttttt aacatagtcg gcgtgccccg
      361 ggcagtctac gtgtgcgtag tgacgggtcg gggtgtcata ttcaacgtga gaagtgttga
      421 tggtgatacc acgagctttt tcttccggcg cgttatcgat ctggtcgaat gcacgagcag
      481 caccgccgta ggttttagcc agtacggtgg tgattgcagc ggtcagagta gttttaccgt
      541 ggtcaacgtg gccgatagta ccaacgttaa cgtgcggttt tgtacgttca aatttttctt
      601 tagacacggc tatattcctt actatagtgc tctccccttc aggagagagc acgggacttt
      661 ggtattaacc cttaggctta tttaccacgg gcttcaatta cggcctgagc aacgttactc
      721 ggcgcttcat catacttcag gaattccata gtgtatgatg cacgaccttt ggtcagagaa
      781 cgcagctgag ttgcgtatcc gaacatttca gacagcggta cttcagcgtg gatcttaacg
      841 ccagtaactt cagattcctg acctttgagc ataccacgac gacggctcaa gtcaccgata
      901 acgtcaccgg tgttctcttc cggagtttct acttcaacct tcatgatcgg ctcaagcaga
      961 actggtttcg ctttcttaaa gccttcttta aaggcgatag aagcagccag tttaaacgcc
     1021 agttcagagg agtcaacgtc atggtaagaa ccgaagtgca gacgaatacc catgtctact
     1081 accgggtagc ctgccagcgg acctgctttc agctgttcct ggataccttt atcaacggcc
     1141 gggatgtatt cgccagggat tacaccacct ttaatgtcgt tgatgaactc gtagcctttc
     1201 gggtttgaac ccggctccag cgggtacatg tcgataacaa catgaccata ctgaccacga
     1261 ccaccagact gtttcgcgtg tttaccttca acatcggtaa ctttctggcg gatagtttca
     1321 cggtaagcaa cctgcggttt acctacgttc gcttcaacgt tgaattcacg cttcatacgg
     1381 tcaacgatga tgtcgaggtg cagttcgccc atacccgcaa tgatggtctg gttagattct
     1441 tcgtcagtcc atacacggaa agacgggtct tctttagcca gacggcccag agccagaccc
     1501 attttttcct ggtcagcttt ggttttcggt tcaactgcga tggagattac cggctcaggg
     1561 aattccatac gttccagaat gatcggcgca tccgggtcac acagggtgtc accagtggtt
     1621 acgtctttca gaccgatagc agcagcgatg tcgcctgcgc gaacttcttt gatctcttca
     1681 cgtttgttag cgtgcatctg aacgatacga ccgaaacgct cacgtgcagc tttcacggag
     1741 ttcagtacgg tatcaccaga gttaaccaca ccggagtaaa cacggaagaa ggtcaggtta
     1801 ccaacaaacg ggtcggtagc gattttgaac gccagtgcag agaacggctc gtcatcactt
     1861 gcgtgacgtt cagccggagt gtctttaccg tcgtccagga taccgttgat cgcaggtacg
     1921 tcaaccgggg atggcaggta atcaattacc gcatccagca tcgcctgaac acctttgttc
     1981 ttgaacgcag aaccacaggt taccaggatg atttcgttgt tcagaacgcg ctgacgcaga
     2041 gcacctttga tttctgcttc agtcagttct tcaccaccca ggtatttttc catcagctct
     2101 tcagaagctt cagctgcgga ttcgatcagg ttctggtgcc attcgttagc cagttcaacc
     2161 atgtctgccg ggatatcttc gtattcgaag gttacgccct ggtcagcgtc gttccagttg
     2221 atagctttca ttttcaccag gtcaacaaca ccggtgaaat gttcttcagc accaatcgcc
     2281 agctgcagcg gaaccgggtt cgcgcccaga cgggttttga tctggttaac aactttcagg
     2341 aagttcgcac ccatacggtc cattttgtta acgaacgcaa tgcgcggaac tttatatttg
     2401 tttgcctgac gccatacggt ttcagactgc ggctgaacac caccaactgc gcagtaaacc
     2461 attaccgcac catcgagcac acgcatggaa cgttctactt cgattgtgaa gtcaacgtgc
     2521 cccggggtgt cgatgatgtt gatgcgatgc ggctcatact gcttagccat accagaccag
     2581 aatgcagtag tcgcagcgga agtgatggta ataccacgtt cctgctcctg ctccatccag
     2641 tccatggttg cagcgccgtc atgaacttca ccgattttat ggtttacacc ggtgtagaac
     2701 agaatacgtt cggtagtagt ggttttaccg gcgtcgatgt gcgcactgat accgatgtta
     2761 cggtagcgtg cgatgggtgt tgtacgagcc atttgtttcc tcgtttatct tttaggcgtt
     2821 caatttaagt agcccaaagc gggctgctta ctggaagcgc ccgcctggtg actaaaactc
     2881 cgaagggatt accaacggta gtgtgcgaac gccttgttgg cttcggccat acggtgaacg
     2941 tcttcacgtt tcttaactgc agtacctttg ttttctgcag catcagaaag ttcgttcgcc
     3001 aggcgcagag ccatggattt atcaccgcgt ttacgagcag cttcaacgat ccaacgcatt
     3061 gccagagcat tacgacgaac cggacggact tcaactggta cctgataagt agaaccacca
     3121 acgcggcgag acttaacttc tacagtcggg cgcacgtttt cgagagctac ttcgaatgct
     3181 tccagttcag atttaccaga gcgctgagcc agggtctcca gcgcgctgta tacgatagat
     3241 tcagcagtag attttttacc atctaccatc aggatattta caaatttagc cagcagttct
     3301 gatccgaact tcggatccgg cagaatttta cgctgaccaa tgacgcgacg acgtggcatg
     3361 gaaatactcc gttgttaatt caggattgtc caaaactcta cgagtttagt ttgacattta
     3421 agttaaaacg tttggcctta cttaacggag aaccattaag ccttaggacg cttcacgcca
     3481 tacttggaac gagcctgctt acggtcttta acgccggagc agtcaagcgc accacgtacg
     3541 gtgtggtaac gaacacccgg gaggtcttta acacgaccgc cacggatcag gatcacggag
     3601 tgctcctgca ggttgtgacc ttcaccaccg atgtaggaag tcacttcgaa accgttagtc
     3661 agacgaacac ggcatacttt acgcagcgcg gagttcggtt ttttaggagt ggtagtatat
     3721 acacgagtac atacgccacg tttttgcggg catgcttcca gcgcaggcac gttgcttttc
     3781 gcaactttgc gagcacgtgg tttgcgtacc agctggttaa ctgttgccat taaatagctc
     3841 ctggttttag cttttgcttc gtaaacacgt aataaaacgt cctcacacaa tatgaggacg
     3901 ccgaatttta gggcgatgcc gaaaaggtgt caagaaatat acaacgatcc cgccatcacc
     3961 aggccatctg gccggagtgc ttaaccgtaa gtctgacgaa atcagtatag tcaatgggaa
     4021 tgatgtcgtt cgaaatttga ccagtcaaac cgcgggcaat aaggtcttcg ttcagggcat
     4081 agaccttaat gggggcatta cgcagacttt caaggtagcg gttaccgtca actgcggcag
     4141 ttacgccatc ttgcaataat agcagttcgt cgccttcact gagcagacgc agcagcgcag
     4201 caaaatccgt cagccagggt gaacgatgta acgtgtgcag cataaacgcc tcaaaacctc
     4261 aaaataacat cgtagttggc gagttcgcgg cgtaaggcat ctgcttcgag cggcgtggct
     4321 tcgacaacaa agggggtctg cggatctaac ccgcgttcgc gcagtgaagc cgcacaaacc
     4381 cagcactgtt caatgtcgta cagatccaac aatttaaaag tggcaatgta atcacgcgcc
     4441 agcactgcat cgggcttttg tcctggcagt agctgaaaaa cgccatcagc aataaagaag
     4501 acggccagct cgtcagttaa cgcggaagtt gccagtaaag catctaaacc ttcccgcccg
     4561 gcagcggtac catgaggcac agtagaaaaa acaaacgcaa ttcgtttcat cagaactgta
     4621 ccaccctgtc acaggtcagc gaggcttccg ccagcgcgcc aagtccgctt aaggtaaatc
     4681 cctgctgaag gtttgacgaa gccagcccca gtcttccggc ctccatttca tcaacaacgc
     4741 cacggcgtaa tgctgccgct acgcagatat tcagcgccac accatgttgc gcattcaact
     4801 gttgccagcc gcgcacaagg tcaaattcgt cgcttgccgg tgaggtcaag tggttagcgt
     4861 tatagacccc ttcccgatag aaaaagacgc tgcttaactt atggccttct gctatcagcg
     4921 cctgcgcaaa ctgaaaagca ctactcgcct gttgcgtacc gtatgctggc ccggtcacca
     4981 cgatggcaaa acgcattact tatcttgccc ctggaaatca ccgctcttga actggcggat
     5041 gtagagatag acagtgtgtt tggagatgtt caggcggtca gcaacctggt tgatcgcatc
     5101 tttaatatcg aagatccctt tctcgtagag attcagcacg atctgacgat ttttggcgtt
     5161 attagaaaca ttgcgatcgg cattcacttc ttcgatggtg aactccagcg tttgggtaac
     5221 cagatcttca acagaagagg caaagttgac gcttgaaccg acatccgggg tttctggcgg
     5281 cacaaaggtg ctcataatct gcgagaaggg aacatcaaga ttcatattga tgcacagcag
     5341 accaattaca cgctgttcgc ggttacgaat cgcgatagtc agggacttca ttaatacgcc
     5401 gcttttggcg cgagtaaagt agcatttaga aacgctgcta tccgctcccg tcatatcgtg
     5461 cagcatacgt agcgccaggt cagtaattgg cgaaccaatc ttccggcctg tatgttcacc
     5521 gttagcaatg cgaatggctg aacattttag atcctgcaaa gagtgcaaaa cgatttcaca
     5581 gtgggagcca ataagcatcg ctaacccgtc caccaccgct tcgtaggatt tcagaatatc
     5641 aaaatcggtc tgctcgaaag gacgttgatc cagtaaatcc aactcactgg tttcgttggt
     5701 taaaagcgac ctggacatga aaaaaaacac tccttttcag gagcctgtcg ttaacttttc
     5761 agggcaggct cattaatgat gcgggtaact aaattaatac agcggaggtt ccgctttcca
     5821 gcactaatta tatccggcct gtaataaaaa aaccgccgcc tggtcaggcg gcggttctta
     5881 atgcttattt tttagcagaa tctgcggctt tcgcatcagc ttccggcttt gcatcagcct
     5941 tcggcgctgg tttcacatcc agcagctcta cgtcaaacac cagggtagaa ttcggtggga
     6001 tccccggaac acccgctttg ccgtaagcca gttctggtgg aataaccagt ttgatcttac
     6061 cgcctttctt gatgttcttc agaccttctg tccaacccgg gataacaccg tccagacgga
     6121 aagagagcgg ttcaccacgg gtgtaagagt tgtcgaactc tttaccgtcg atcagcgtac
     6181 ctttgtagtt cacgacaaca gtatcgctgt ctttcggtgc ttcgccttta ccggcttcta
     6241 ctacctgata aaccagacca gtggaagagg ttttcacacc tttctctttg gcaaatttct
     6301 cgcggtactc tttacctttt gcttcgttat cagccgcgtc tttttccatc ttcgcctgag
     6361 cagaagactt cacgcgagct tcgaatgctt gcagagtctg ttcgatctct tggtcggaga
     6421 gtttgctctt atcagcaaat gcatcctgaa caccagcgat cagctgatct ttatccagtt
     6481 tgatgcccag tttttcttgt tcttttagag agttttccat gtaacgaccc agcgaggcac
     6541 ccagtgcata agctgatttc tgatcgtcat ttttgaacgc tgctttgctg tcagcagttg
     6601 tagcaggttt tgcagcttca gcagcaaaag tgattggtgc atgcaaggca acggccattg
     6661 tggtcgccag cagcgttact ttaaacagtg atttcatcca tatctccagg atcggggcat
     6721 ctcaccccag ggttaactat tatcagaagg gtactataaa gcgttgtcga acaaatctac
     6781 atacagacac gccctattat catctacttt cagactcttt ttgtttaaat tagtttcgat
     6841 gaccgcgaaa tgagtgctgt ctcgggcagc aaagttaagt agaatccgcg gcgaccattc
     6901 gacaaaagag gtgaatcatg caggatttat cattggaagc acgcctggca gagctggaga
     6961 gccgactggc ttttcaggaa atcaccattg aagaactgaa cgtcacggtg accgctcatg
     7021 aaatggagat ggcgaaactg cgcgatcatc tgcgtctgct gaccgagaag ttaaaagcca
     7081 gccagccgtc gaacatcgcg tcgcaggctg aagaaacgcc accgccacat tattgaggcg
     7141 taaaaaaagc ggggattccc cgcttttttg tcaccttttc ggtattagtg gcaaccgcaa
     7201 ccgccgttgc ctttaccgcc acagcagcct tcgccaccgt gttcatgacc gtgatcgtgg
     7261 ccatgaccgc cgcagcaacc gtcgtggtcg tgatcgtggt gatgatcgtg cgcgccgtga
     7321 acgtggccat gagccagttc ttcttcggtc gcttcgcgaa tcgccacaac ttcaacgttg
     7381 aatttcaggt tctgaccggc cagcatgtgg ttaccatcaa ccacgacgtg atcgtcttca
     7441 accgcagtga tttcaaccgg taccggaccc tggtcggttt cagccaggaa acgcatacct
     7501 acctgcagtt catcaacgcc cataaatacg tctttaggaa cacgttgcac caggttttcg
     7561 tcgtactgac cgtaagcgtc gttcgcgcca acagcgacat caaatttgtc gccaacttca
     7621 tgaccttcca gcgccgtttc caggccagag atcagggaac cgtgaccatg caggtagtcc
     7681 agcggcgcac tcaccggaga ctcatcaacc aacacaccgt cttctgtacg tacctgatag
     7741 gccaggctga ccaccaggtc ttttgctact ttcatgatat ctcctgagca tgggaagaat
     7801 agtggcgcag attgtagcgg aattctgcag ccgtgtactc actagcttaa aaaaactcgg
     7861 cgcatatcgc tagtccggat gaaaaatccc gatcacttgc tcatctttgc gaacgtgatc
     7921 gcgggcttct ttgtctgctt ctcgcatctg gtgtccgcac ttaacgcatt caacaatatc
     7981 aatattattt tcgcgccaca tcgccattga atcctgcgcc tgacaggccg ggcattttgc
     8041 gcccgcaata aaacgttttc ggattgccat ctttaccctc tactcaaatt catcccagcc
     8101 gtccagctag cgtcgttctt gttgcatttc acgctggaaa atctcttcca gttcgcgtcg
     8161 ggcttccctg gcgcgagaaa tttgtacggt atcggcatgc attgggataa gctctcgcag
     8221 catccgcata tccaggcggc gaaaatgcag ttgcgcccgc tgtgcctgat gcggatgcat
     8281 accaagcgtg accagcgtct tgcgccccag ctctaacgca ctggagaatg tttcacggga
     8341 aaactgcgtc acccctgcct gtaataactc atgcgcttcc acacgtccgc gcgctcgcgc
     8401 aagaatatgc aaatgcggaa agtgctgctg gcatatttcc accagcttca tggtgtcttc
     8461 cggctcgtta caggtaatga cgatagactc agcggcctct gcacccgcag aacgtaaaag
     8521 atctacctgc gtggcatcac cgtaataaac tttgtagcca tatttgcgca tcaggttaac
     8581 ggcgctgata tcccgctcca gcacagtaat gcgcatttta tttgccatca acaaacgacc
     8641 aatcacctgc ccaaaacgtc caaagcccac gacaatgacc tggggtttat catcgttgac
     8701 ccacggtttt tcgtcttctt cttccggtcc gttaaactgg cgggatagcc atttatccac
     8761 cagcttcatc agcagcggcg tggtcatcat ggaaagcgtc accgtcacca gcaacagcgc
     8821 catctggtcg ccctggaata agcgttgtga agaagcggta gaaaagagga caaaggcaaa
     8881 ctcaccgccc tgactcaaca cgccagcaaa ctgcatccgc tctgagctac gcacgccata
     8941 caatcgcgcc agcagataca gcacgagaat tttcaccgcc accagcacaa ccacgcttat
     9001 cactacccac aacagatggg tataaagcac cccgaggttg agtgacatcc cgacagagat
     9061 aaagaacaaa ccgagcagca agcctttaaa gggatcgata gccgtttcca gttcatggcg
     9121 atattcactt tccgccagca gcacgcctgc aataaacgta ccgagcgcca tcgacagccc
     9181 caacgcatcc ataaacaatg cggaacccaa taccagcagc agcgtcgcgg cggtgaacac
     9241 ttcccgcacg ccagaagctg caataaaacg gaataccgga cgcaataaat agcgcccgcc
     9301 aatcagcatg ccgacaaacg ccagcacctt catgccgatc ttcatccagt cgaaatgttc
     9361 gtctgccgaa cccgccaaca acggcactaa cgccagtgcc gggatcaccg ccagatcctg
     9421 aaacagcagt accgaaaacc cgagctgccc ggattcgctg cgattcatcc ctttctcacg
     9481 catcaattgc aacgccattg cggtcgaaga catcgcaagg ccaatgccac cgaccaccgc
     9541 cgcctgccag gcgaaatccg tcagcatcaa taatcccgcc agcaacgccg cgcttaacag
     9601 cacctgtgcc gcgcctacgc caaaaatcga acgccgcagt tgccaaagct tggagggatt
     9661 caactcaagg ccgatgataa acatcaggaa caccacgccg agttccgaaa agtggaggat
     9721 ctcatcgacg tcgctaataa accccagccc ccacgggcca attgcaatcc ctgccagcaa
     9781 atatcccaac acagcgccaa tacccagccg cgatgccaac ggcaccgcag ccaccgccgc
     9841 gaagagaaac agcactcctg caagtaaaaa atcggaacct tccatcagcg gcctcctgga
     9901 gacagcggat ttgccagcca gtcaccgtag gctctggcgt ggctcgccag ctcctgtgcg
     9961 ctttgccgtc tcgcccagta aataatgatg ggacttaacc aatgcatccg gcacatgccc
    10021 gccgccagtt caaaggggcg caggacatcg ctcatcgggt aacgattcaa cgcgtcataa
    10081 cggtaagcac tttccggctc gccggtggta atcacactac gccagtactt tcccgccagt
    10141 tggtttcctc ccggcccgct ggcaaaacca cggcttaata cccggtccag ccactctttc
    10201 agtagcgccg ggcagctata ggtataaaga ggatgctgaa agacaatcac ctcgtgctcg
    10261 cgcagtaatg cctgctcacg ggggatatca ataaaaaaat cgggatagtg cgcgtaaagg
    10321 tcgtgcacgg taacattgct gagctgcgtg gccggtttaa gcagtacccg gtttgccacc
    10381 gagtcctgag attccggatg ggcatacagc agcaaaactt tcgctggctg agacatcatc
    10441 cccctcccgg tatggttttt gtgtatagtc gctgttttgg gctaccattg cgcccggtgc
    10501 ggcagctcgc ccatacatta cattatcata atgataagtt aacatagtct gaacatacgg
    10561 caccttatga ttgttttctc ctcgttacaa attcgtcgcg gcgtgcgcgt cctgctggat
    10621 aatgccaccg ccaccatcaa ccccgggcag aaagtcggcc tggtgggtaa aaacggctgt
    10681 ggtaaatcta ccctgctggc attgctgaaa aatgaaatca gcgccgacgg cggcagctac
    10741 acctttccgg gaagctggca actggcgtgg gtgaatcagg aaacgccggc gttaccgcaa
    10801 gcggcgctgg aatatgtcat tgacggcgac cgtgaatatc gtcaactgga agcgcagcta
    10861 cacgacgcca acgaacgtaa cgacgggcac gccattgcga ccattcatgg caagctggat
    10921 gctattgacg catggagtat tcgctcccgt gccgccagcc tgctgcacgg cctcggtttc
    10981 agcaatgaac aactggagcg cccggtaagt gatttctctg gtggctggcg tatgcgtctt
    11041 aaccttgccc aggcgctgat ttgccgttca gacttgctgc tgctcgacga accgactaac
    11101 cacctcgatc tcgatgccgt tatctggctg gaaaaatggc tgaagagcta tcagggcacg
    11161 ctgatcctga tctctcacga ccgcgacttc ctcgatccga tcgtcgataa aattattcat
    11221 atcgaacaac aaagcatgtt cgagtacacc ggcaactaca gttcgtttga agtacagcgc
    11281 gccacccgtc tggcgcagca acaagcgatg tacgaaagcc agcaggaacg cgtagcgcat
    11341 ctgcaaagtt atatcgaccg tttccgtgcc aaagccacca aagcgaagca ggcccagagc
    11401 cgcattaaga tgctcgagcg tatggagctg attgcccccg cgcacgtcga caacccgttc
    11461 cgctttagct tccgcgcgcc ggaaagcctg ccaaatccgt tactgaagat ggaaaaggtc
    11521 agcgcaggct atggcgatcg cattattctc gactcgatta aactgaatct ggtccccggt
    11581 tcgcgcattg gtctgttagg ccgcaacggc gcgggtaaat cgacattaat caaactgtta
    11641 gccggtgaac ttgcgccagt cagtggtgag ataggcctgg caaaagggat caagctcggc
    11701 tacttcgccc agcatcaact tgaatacctg cgcgccgacg aatcgcctat tcaacatctg
    11761 gcacgtttag cgccgcagga actggagcaa aaactgcgtg actacctcgg cggctttggt
    11821 ttccagggcg ataaagtaac cgaagaaacg cgccgcttct ccggtgggga aaaagcccgc
    11881 ctggtgctgg cattaatcgt ctggcagcgt ccgaatctgc tgctgctcga cgaaccgact
    11941 aaccaccttg accttgatat gcgtcaggca ctcaccgaag cattaatcga gtttgaaggc
    12001 gcgctggttg tcgtttcgca cgaccgtcat ttgctgcgtt ccaccactga cgatctctac
    12061 ctggttcacg atcgtaaagt cgaaccgttc gacggcgatc tggaagatta tcaacagtgg
    12121 ttgagcgacg tacaaaagca ggaaaaccag accgacgaag cgccaaaaga gaacgcgaac
    12181 agcgcccagg cacgtaaaga tcagaagcgt cgggaagcgg agctgcgtgc gcaaacccag
    12241 ccactgcgta aagagattgc ccgtctggaa aaagagatgg agaagctgaa cgcgcaactg
    12301 gcgcaggcag aagagaaact cggcgacagc gaactgtatg accagagccg taaagcggag
    12361 ttgaccgcct gcctgcaaca gcaagccagc gccaaatccg gcctggaaga gtgcgaaatg
    12421 gcatggctgg aagcccagga gcagcttgag cagatgctgc tggaaggcca aagcaactga
    12481 tggcgcagat aacgacgacc gatgccaatg aattcagcag cagtgctgaa ttcaccccta
    12541 tgcgcggctt tagcaattgt catctgcaaa ccatgctgcc gcgtctgttt cgtcgcaagg
    12601 tgaaattcac cccgtactgg cagcgactgg agttgcccga cggcgatttt gtcgatctcg
    12661 cgtggagtga agcccctgca caggcgcaac ataaacctcg tctggtggtg tttcacgggc
    12721 tggaaggcag tctcaatagc ccttacgccc acggtctggt tgaggcggcg caaaagcgcg
    12781 gctggctggg cgtggtgatg cattttcgcg gatgcagcgg tgaaccaaac cgtatgcacc
    12841 gcatttacca ttcgggcgaa accgaagacg ccagttggtt tttacgctgg ctgcaacgcg
    12901 agtttggcca tgcgccaacg gctgccgtcg gctattcgct cggcggtaat atgctggcct
    12961 gtttgctggc aaaagaaggc aatgatctcc cggttgatgc ggcggttatt gtctctgcgc
    13021 cgtttatgct ggaagcctgt agctatcata tggaaaaggg cttttcccgc gtttatcagc
    13081 gttacttgct gaacctgtta aaagccaatg ccgcgcgcaa gctggcagcc taccccggaa
    13141 cactgccgat taatctcgcg cagttaaaat cggtacgtcg catccgtgaa tttgacgatc
    13201 tcatcaccgc cagaattcac ggctacgctg acgctatcga ctattatcgt cagtgtagcg
    13261 ccatgccgat gctgaaccgg atcgccaaac cgacgctgat tattcacgcc aaagacgatc
    13321 cgtttatgga tcatcaggtg atcccgaaac cggaaagtct ccccccgcag gtggagtatc
    13381 aactgactga acatggcggt catgttggct ttattggcgg tacattactt catccgcaaa
    13441 tgtggctgga gtcacgcatt cctgactggt taacaacgta tctggaggcg aaatcatgtt
    13501 gattccgtgg caagacctct cccccgaaac gctggaaaat ttaattgaaa gctttgtgtt
    13561 acgtgaaggc accgattatg gtgaacatga gcgtacactt gaacagaaag tcgccgacgt
    13621 caaacgccag ctacagtgcg gagaagcggt gctggtatgg tcggagctgc acgaaacggt
    13681 caatatcatg ccgcgcagtc agtttcgcga ataacctgca accgtcggcg gacgagataa
    13741 taatgatagc taagtcatgg agttactatg tctgccaaac atccggtcat tgcggtaaca
    13801 ggatccagcg gcgcggggac caccaccacc agcctcgcgt ttcgtaaaat attcgcgcag
    13861 ttaaatctgc atgcagccga ggtggaaggc gacagttttc accgttacac ccgcccggaa
    13921 atggacatgg cgatccgcaa agcgcgcgac gccgggcggc atatcagcta cttcggcccc
    13981 gaagcgaacg acttcggcct gctggaacaa accttcattg aatacggtca gagcggcaaa
    14041 gggaaatctc gcaaatatct gcatacctac gatgaagccg taccgtggaa tcaggtaccg
    14101 ggaacattca ccccctggca acctttacca gaacccactg atgtactgtt ttatgaaggt
    14161 ttacacggcg gcgtagtcac gccacagcat aacgttgcgc agcatgtgga cttactggtc
    14221 ggcgtggtac ctatcgttaa ccttgagtgg attcaaaaac tgatccgcga caccagcgag
    14281 cgcgggcact cacgagaagc agtgatggat tcagtagtgc gttcaatgga agactatatc
    14341 aactacatca caccgcagtt ttcccgcacc catcttaact tccagcgcgt tcccaccgtc
    14401 gacacttcaa acccgttcgc ggcaaaaggt atcccgtcgc tggatgaaag ctttgtggtg
    14461 atccattttc gtaatctgga agggatcgat ttcccctggc tgctggcgat gttgcaaggc
    14521 tcattcattt cccacatcaa tacgttagtg gtgcccggcg gcaaaatggg tctggcaatt
    14581 gaattaatta tgctgccgct ggtgcaacga ttgatggaag gaaagaaaat cgagtaactc
    14641 tgctattacg ccggataaaa tactatccgg cttcacaacg ggatagttaa atcacgcggc
    14701 aaccacttca tacgagtgag taatattcac cgctttttcc agcatcaacg ccactgaaca
    14761 atatttctca gcagagagat caaccgcacg cgcaaccgct gcgtctttca ggtcgcgacc
    14821 ggtgacgata aaatgcagat taatgtgcgt aaacaggcgt ggtgcctctt cgcggcgttc
    14881 agaggtcaat tttacttcac aatcgaccac atcctgacgc cctttttgca ggatcgaaac
    14941 cacatcgatg gcactgcaac cacccgccgc catcaacacc atttccatcg gacttggtgc
    15001 tttatcgcct gagttgccgt ccattaaaat ctggtgacca gaggcagatt cgcccagaaa
    15061 agttaaccct tcgacccact tcactcgcgc ttgcattttt gctactccac tgcgtcaatt
    15121 ttcctgacag agtacgcgta ctaaccaaat cgcgcaacgg aaggcgacct gggtcatgct
    15181 gaagcgagac accaggagac acaaagcgaa agctatgcta aaacagtcag gatgctacag
    15241 taatacattg atgtactgca tgtatgcaaa ggacgtcaca ttaccgtgca gtacagttga
    15301 tagccccttc ccaggtagcg ggaagcatat ttcggcaatc cagagacagc ggcgttatct
    15361 ggctctggag aaagcttata acagaggata accgcgcatg gtgcttggca aaccgcaaac
    15421 agacccgact ctcgaatggt tcttgtctca ttgccacatt cataagtacc catccaagag
    15481 cacgcttatt caccagggtg aaaaagcgga aacgctgtac tacatcgtta aaggctctgt
    15541 ggctgtgctg atcaaagacg aagagggtaa agaaatgatc ctctcctatc tgaatcaggg
    15601 tgattttatt ggcgaactgg gcctgtttga agagggccag gaacgtagcg catgggtacg
    15661 tgcgaaaacc gcctgtgaag tggctgaaat ttcgtacaaa aaatttcgcc aattgattca
    15721 ggtaaacccg gacattctga tgcgtctgtc tgcacagatg gcgcgtcgtc tgcaagtcac
    15781 ttcagagaaa gtgggcaacc tggcgttcct cgacgtgacg ggccgcattg cacagactct
    15841 gctcaatctg gcaaaacaac cagacgctat gactcacccg gacggtatgc aaatcaaaat
    15901 tacccgtcag gaaatcggtc agattgtcgg ctgttctcgt gaaaccgtgg gacgcattct
    15961 gaagatgctt gaagatcaga acctgatctc cgcacacggt aaaaccatcg tcgtttacgg
    16021 cactcgttaa tcccgtcgga gtggcgcgtt acctggtagc gcgccatttt gtttcccccg
    16081 atgtggcgca gactgattta tcaccccgat attaactatg cactgcgaca aacgctggtg
    16141 ctatgtttgc ccgtggccgt tgggttaatg cttggcgaat tacgattcgg tctgctcttc
    16201 tctctcgttc ctgcctgttg caatattgcg ggccttgata cgcctcataa acgttttttc
    16261 aaacgcttaa tcattggtgc gtcgctgttt gccacctgta gcttactgac acagctgcta
    16321 ctggcaaaag atgttcccct gccttttttg ctgaccggat taacgctggt acttggcgtc
    16381 actgctgagt tggggccatt gcacgcaaaa ttgcttcccg catcgctgct ggcagccatt
    16441 tttaccctca gtctggcggg atacatgccg gtctgggaac cgttgctcat ctatgcgttg
    16501 ggcactctct ggtacggatt gtttaactgg ttttggttct ggatctggcg cgaacaaccg
    16561 ctgcgcgagt cactaagtct gctgtaccgt gaactggcag attattgtga agccaaatac
    16621 agcctgctta cccagcacac cgaccctgaa aaagcgctgc cgccgctgct ggtgcgccag
    16681 caaaaagcgg tcgatctaat tacccagtgc tatcagcaaa tgcatatgct ttccgcgcaa
    16741 aataatactg actacaagcg gatgctgcgt attttccagg aggcgctgga tttacaggaa
    16801 catatttcgg tcagtttgca tcagccggaa gaggtgcaaa agctggtcga gcgtagccat
    16861 gcggaagaag ttatccgctg gaatgcgcaa accgtcgccg ctcgcctgcg cgtgctggct
    16921 gatgacattc tttaccatcg cctgccgacg cgttttacga tggaaaagca aattggcgca
    16981 ctggaaaaaa tcgcccgcca gcatccggat aatccggttg ggcaattctg ctattggcac
    17041 ttcagccgca tcgcccgcgt gctgcgcacc caaaaaccgc tctatgcccg tgacttactg
    17101 gccgataaac agcggcgaat gccgttactt ccggcgctga aaagttattt gtcactaaag
    17161 tctccggcgc tacgcaatgc cggacgactc agtgtgatgt taagcgttgc cagcttgatg
    17221 ggcaccgcgc tgcatctgcc gaagtcgtac tggatcctga tgacggtatt gctggtgacg
    17281 caaaatggct atggcgcaac ccgtctgagg attgtgaatc gctccgtggg aaccgtggtc
    17341 gggttaatca ttgcgggcgt ggcgctgcac tttaaaattc ccgaaggtta caccctgacc
    17401 ttgatgctga ttaccaccct tgccagctac ctgatattgc gcaaaaacta cggctgggcg
    17461 acggtcggtt ttactattac cgcagtgtat accctgcaac tgttgtggct gaacggtgag
    17521 cagtacatcc ttccgcgtct tatcgatacc attattggtt gtttaattgc tttcggtggt
    17581 accgtctggc tgtggccgca gtggcagagc gggttattgc gtaaaaacgc ccatgacgct
    17641 ttagaagcct atcaggaagc gattcgcttg attctgagcg aggatccgca accaacgcca
    17701 ctggcctggc agcgaatgcg ggtaaatcag gcgcataaca ctctgtataa ctcattgaat
    17761 caggcgatgc aggaaccagc gttcaacagc cattatctgg cagatatgaa actgtgggta
    17821 acgcacagtc agtttattgt tgaacatatt aatgccatga ccacgctggc gcgggaacac
    17881 cgggcattgc cacctgaact ggcacaagag tatttacagt cttgtgaaat cgccattcag
    17941 cgttgtcagc agcgactgga gtatgacgaa ccgggtagtt ctggcgatgc caatatcatg
    18001 gatgcgccgg agatgcagcc gcacgaaggc gcggcaggta cgctggagca gcatttacag
    18061 cgggttattg gtcatctgaa caccatgcac accatttcgt cgatggcatg gcgtcagcga
    18121 ccgcatcacg ggatttggct gagtcgcaag ttgcgggatt cgaaggcgta atgcaggcta
    18181 aaccatcgga taaggcatgc acgccgcatc cgacatcttt tgcctgatgc gtcttattta
    18241 cgccccaacc accttcgcca ccgcgtgggc gaaacgttgc atcccttcat cgatatccgc
    18301 atcttccacc accagcgacg gcgcaaaacg catcacatcc ggtccggcat tcagcaccat
    18361 tacgccagcc tctgcgcccg catacaggaa atcacgcgcc tgacctttgt actgtggttt
    18421 cagctccgcg ccaattaaca gccccatacc gcgaatatcg ctaaacacat cgtactgctg
    18481 atcgatcttc tgcaaatggt caacaaaacg ctggcgtttc gcctgaatgc cttccagcac
    18541 ttcaggggta ttaatgatat caaacgccgc gcccgctact gcacaggcca gaggattacc
    18601 gccgtaggtg gaaccgtgag aaccaggatg aaacgcagaa gcaatttccg ccgtggtcag
    18661 catggcgcta atcgggaagc cgccgcctaa cgctttcgca gaagtcagaa tatccggcgt
    18721 cacgccgtag tgcatgtaag caaacaaatc gccagtccgc cccatcccgc actgtacttc
    18781 atcaaacacc aataatgcct gatgttgatc gcacaactca cgcaagccct gcaaaaactc
    18841 tggcgtcgct gccgtcacac cgccctcgcc ctggatcggc tcaaccacca ccgcacaggt
    18901 gtgatcatcc atcaccgctt tcactgcgtg gagatcgtta aagggaacgt ggatgatgtc
    18961 tgatggtttt ggcccaaagc cgtcggaata ttttggctgc ccacccaccg aaacggtaaa
    19021 cagcgagcga ccatgaaaag cgttatggaa ggcaataatt ttggttttga acgggctatg
    19081 acgcacgcag gcgtaatggc gtgccagttt aaaggcggtt tcgttagctt ccgtgccgga
    19141 gttcataaac accacgcgtt cggcaaacgt cgcctcaatc agtttacgcc caagacgcag
    19201 cgccggttca ttggtgaaaa cgttactgat atgccacaga gtttcgccct gggtttttaa
    19261 cgcgttcacc agcgcaggat ggcaatggcc caacgccgta actgcaatgc cacccgcgaa
    19321 atcgacatac tccttgcctt gctgatccca gattcggctg ccctgacctt ttaccggaat
    19381 gaactctgcc ggagcataaa tcggcaggat cacttcatcg aaagtcgcgc gtgtaattgc
    19441 tgtttgttca attgccatct catgatcacc ctgttatgca taaataaatg tgaaattata
    19501 atcacaaaat atgcataaaa aatcattgaa tggcaatcag aaatcagcga tgcaggaaat
    19561 tagccagcag ttgatgtcct tgttcgctaa gaatactttc tggatggaac tgcacacctt
    19621 ccagatccca ctggcgatgg cgaatcccca taatctcgcg ggtttcgctc caggccgtca
    19681 cttcaaagca cgctggtaac gagtcaggtt ccactaccag cgaatgatag cgtgtcacgg
    19741 taagtggatt tgccagcccc cgaaatacgc cctcaccgtt atgtgtaatc ggcgaggttt
    19801 tgccgtgcat gacctttgcg gcgcgcacaa ctttaccgcc aaatgcctgc gccattgcct
    19861 gatgaccgag gcagacgcca agaatcggca agcgcccggc atagtggcga ataacgtcaa
    19921 gggagatccc ggcttcatct ggcgtacagg ggccgggtga gatgacaact ttttgtggtt
    19981 taagggcgtc gatatccgcc agcgtcaacg catcgttgcg cttaaccagc acatccgccc
    20041 ccagttcaca aaagtactgg tagaggttcc aggtaaaaga atcgtagtta tctataagca
    20101 ggatcatggc ggctccggta cgaaagaacc gcgctatttt actcagattc cccggcttcg
    20161 cttaccactt tgctaaatat catctgcaac gcggtgagat cccccattgc cccactctga
    20221 tttgcctgat tccaggcctc tttttcgata ccctgccagc tcagttgata tccggcatga
    20281 atcgccagtt gctcgaagaa gatccgctgt gccagaccac ttcccacccg gaagggatgc
    20341 agcacgttga tttcacaata gtaatgcgcc agccgctcga cgaacttcgt tttctccagg
    20401 ccaaccagat aaccttcttc ctccagatcc tgcatcaggg cattgccctc tttttcgata
    20461 taagcaaagt ggcagaacgg cgtatcgccc tgataaatat caacttcacg cagttgccct
    20521 gcccagtcga aaatatcctg atacagctgg cgatggatag tgcgcaaatg cggtaaaccg
    20581 cgcaccagcg gaccaagctc aatggtcgca gcacgcagcg ccgtcatttc gtaagcggcc
    20641 tgttccagcc gttgttgctg gcggatgttc agccggttac gcatgatatc aaggccagga
    20701 taaagatacg gatcgcgccc ttcgccgaat ttatcgctca tagtgactcc tcagctcttc
    20761 aaggcgcgct aaagcctctg cggcagtaag agtgactaaa ggcatctcga cgccttcaag
    20821 acggcgactg gcctgaaaat tacgattacg ctgaagctcc cagagacggg acttttgctt
    20881 atcggtgagt ttcttcactt gatgcctccc tgaacgtgtc catttgccac aagtataagc
    20941 ggcaaatccg ggttacgccg ggagagcaga agcgcgggca agattgcccg cgcgagaaat
    21001 cattacggca ggactttagc ggaaaggata actaccggtt ttgacggcac attctggtac
    21061 ggaccaacgt catgagtcgg cacctgggaa atcttatcgg caacgtccat gcctttcacc
    21121 actttaccaa ataccgcgta accgaaatca cgctgaccat ggtcaaggaa ggcgttatcg
    21181 gcaacgttga taaagaactg gctggtggcg ctgtctttgt cagcggtacg cgccatcgcg
    21241 atggtgccac gcgtgttgcg aaggccgtta tcggcttcat ttttgattgg cgggtttggt
    21301 tttttctgct gcatctgctc ggtgaaaccg ccgccctgaa tcataaagcc aggaatgacg
    21361 cggtgaaagg tagtgttgtt ataaaaaccg ctgttcacat aatcgacaaa gttttgcaca
    21421 gacactggcg ctttttgttt atccagctcc agttcgatgt taccagctga ggttgtcaac
    21481 aatacgtgcg ggtccccttt cgctgccatt gctgcgggag aaagagcaga aagagcgaaa
    21541 acagcagcca tcgccgccag ggtcgatttg aacatgagat ttccttaaca aacagagcag
    21601 aaaaaaagca agtgaagaga ttctaaatag ccttcaagaa caaggccatc cctttaccta
    21661 attttacgta tctgaaatat atgtaacctg acgatcactt tttattccgt gatcaaaatc
    21721 acctcttaaa atgcaattta gcaaccgatt gcaataaaac atttaaacag atcacaaaat
    21781 cacctaaaat cgcccgctcg cggcatatcc cgctatgcca ttttattttt gaacgcaggc
    21841 caatcatgac taacagcaat cgcatcaagc tcacatggat tagctttctc tcctacgcac
    21901 tgactggtgc gttggttatt gtcaccggga tggtgatggg aaatatcgcc gattatttca
    21961 atctgcctgt ttccagtatg agtaacacct tcaccttcct caacgccggc attttaatct
    22021 ctatcttcct caacgcctgg ctgatggaaa tcgtcccgtt gaaaacgcag ttacgttttg
    22081 gctttctcct gatggtgctg gcggttgccg gtttgatgtt cagccacagc ctggcgctgt
    22141 tctcggcggc gatgttcatt ctcggggtgg tcagcggcat caccatgtcg attggtacat
    22201 tcctggtaac acaaatgtat gaagggcgtc agcgcggttc ccgcctgtta tttaccgact
    22261 ccttcttcag tatggctggg atgattttcc caatgatcgc cgcgtttcta ctggcgcgca
    22321 gcattgagtg gtactgggtt tatgcctgca tcgggctggt gtatgtcgct atttttattc
    22381 tgaccttcgg ctgtgagttc ccggcgctgg gcaaacatgc accaaaaacg gatgctccgg
    22441 tagcgaaaga aaagtggggg atcggcgtac tgtttctctc tgttgcggcg ctgtgctaca
    22501 tcctcggtca gttaggtttt atctcctggg tgcctgagta tgccaaaggc ctgggcatga
    22561 gcctgaatga tgctggcacg ctggtgagta acttctggat gtcatacatg gtcggcatgt
    22621 gggcgttcag ctttattctt cgcttctttg atttgcaacg cattctgacc gtactggctg
    22681 gtctggctgc gattctgatg tacgtcttta acaccggaac accggcacat atggcgtggt
    22741 caattctcgc cctgggcttc ttctccagcg cgatctatac caccatcatc accctgggtt
    22801 cacagcagac caaagtaccg tcgccaaaac tggttaactt tgtcctgacc tgcggaacca
    22861 tcggtactat gttgaccttt gtggttaccg gcccgattgt tgaacatagc ggtccgcagg
    22921 cggcactgct tacagcaaac ggtctgtacg ctgtcgtctt tgtgatgtgc ttcctgttag
    22981 gtttcgtcag ccgtcaccgt cagcataaca ccctgacctc tcattaattg ctcatgccgg
    23041 acggcactat cgtcgtccgg ccttttcctc tctaaccccg ctacgtgcat ctatttctat
    23101 aaacccgttc attttgtcta ttttttgcac aaacatgaaa tgtcagacaa ttccgtgact
    23161 taagaaaatt tatacaaatc agcaatatac cccttaagga gtatataaag gtgaatttga
    23221 tttacatcaa taagcggggt tgctgaatcg ttaaggtagg cggtaataga aaagaaatcg
    23281 aggcaaaaat gagcaaagtc agactcgcaa ttatcggtaa cggtatggtc ggccatcgct
    23341 ttatcgaaga tcttcttgat aaatctgatg cggccaactt tgatattacc gttttctgtg
    23401 aagaaccgcg catcgcttat gaccgcgtac acctctcgtc ttacttctct caccacaccg
    23461 ccgaagagct gtcgctggtg cgcgaaggct tctacgagaa acacggcatc aaagttctgg
    23521 tcggcgaacg cgctatcacc atcaaccgtc aggagaaggt gattcactcc agcgccggac
    23581 gtaccgtttt ttatgacaag ctgatcatgg cgaccggttc ctacccgtgg atcccgccaa
    23641 ttaaaggttc tgatactcag gactgctttg tctatcgcac cattgaagac ctcaacgcca
    23701 ttgaatcctg cgcccgtcgc agcaaacgcg gtgccgttgt tggtggcggc ctgttaggtc
    23761 tggaagccgc tggcgcgctg aaaaacttag gtattgaaac ccacgttatc gaatttgccc
    23821 ctatgctgat ggcagaacag cttgatcaga tgggtggcga gcagctgcgt cgcaaaatcg
    23881 agagcatggg cgtgcgcgtt cacaccagca aaaacaccct tgagattgtg caggaaggtg
    23941 ttgaagcgcg taaaaccatg cgttttgccg acggcagcga actggaagtc gactttatcg
    24001 tcttctctac cggtatccgt ccgcgcgata agctggcaac ccagtgtggt ctggacgttg
    24061 ctccgcgtgg gggtattgtc attaatgatt cctgccagac ttccgatccg gatatctacg
    24121 ccatcggtga atgcgcaagc tggaacaacc gtgtatttgg tctggtagca ccaggctaca
    24181 aaatggcgca ggtcgccgtt gaccatattc tcggtagcga aaacgccttt gaaggtgctg
    24241 accttagcgc caagctgaaa ctgctgggcg tagacgtagg cggtattggt gatgcgcacg
    24301 gtcgcacgcc tggcgcacgt agctacgttt acctcgacga aagcaaagag atctacaaac
    24361 gccttattgt cagcgaagac aacaaaaccc tgctcggtgc ggtactggtg ggcgatacca
    24421 gcgactacgg caacctgctg caactggtgc tgaatgctat cgagctgccg gaaaacccgg
    24481 attctctgat tctgccggcg cactcgggta gcggtaagcc gtctatcggt gttgataaac
    24541 tgccggacag cgcgcaaatc tgctcctgct tcgacgtcac caaaggtgat ctgattgctg
    24601 ccatcaacaa aggctgtcac accgttgcgg cgctgaaagc cgaaaccaaa gcgggtactg
    24661 gctgcggtgg ctgtatcccg ctggtcactc aggtactgaa cgcggaactg gcgaaacagg
    24721 gcatcgaagt taacaacaac ctgtgcgaac actttgctta ttcgcgtcag gaactattcc
    24781 atttgatccg cgttgaaggc attaaaacct tcgaagaact gctggcgaaa cacggcaaag
    24841 gttacggttg tgaagtttgt aaaccgaccg tcggttcgct gctggcctcc tgctggaacg
    24901 aatacattct gaagccggaa catactccgc tgcaggattc taacgacaac ttcctcgcta
    24961 acatccagaa agacggcacc tactcggtga tcccgcgctc tccgggcggt gaaatcaccc
    25021 cggaagggct gatggcggta ggtcgtatcg cgcgtgaatt taatctctac accaagatca
    25081 ctggctccca gcgtctggcg atgtttggcg cacagaaaga cgatctgccg gagatctggc
    25141 gtcagctgat tgaagccggc ttcgaaaccg gtcatgccta tgcgaaagca ctgcgtatgg
    25201 cgaaaacctg cgtgggtagc acctggtgcc gctacggcgt tggcgacagc gtcggcctcg
    25261 gcgtggaact ggaaaaccgc tacaaaggca tccgtacgcc gcacaaaatg aagttcggtg
    25321 tctccggctg tacccgtgaa tgttcagaag ctcagggtaa agacgtgggt attatcgcca
    25381 ctgaaaaagg ctggaacctg tatgtttgcg gtaacggcgg catgaaaccg cgtcatgcgg
    25441 atctgctggc ggcggatatc gatcgcgaaa cgctgatcaa atatctcgac cgcttcatga
    25501 tgttctacat ccgtactgcc gacaaactga cgcgtaccgc accgtggtta gaaaacctcg
    25561 aaggcggcat cgattacctg aaagcggtga tcattgacga caaactgggg ctgaacgcac
    25621 atctggaaga agagatggcg cgcctgcgtg aagcggtagt gtgtgagtgg actgaaacgg
    25681 tcaatacgcc gtctgcgcag actcgcttca aacacttcat caacagcgac aagcgtgacc
    25741 cgaacgtgca gatggtgcca gagcgcgaac agcaccgtcc ggcaacgccg tatgaacgta
    25801 tcccagtaac tctggtggag gacaacgcat gagccagtgg aaagacatct gcaaaatcga
    25861 tgacatcctg cctgaaaccg gcgtctgcgc gctgttaggt gacgagcagg tcgcgatttt
    25921 ccgcccgtat cacagcgatc aggtgtttgc gatcagcaac atcgacccgt tcttcgagtc
    25981 cagcgtgctg tcacgcggac tgattgcgga acaccagggc gagctgtggg tcgccagccc
    26041 gctgaaaaaa cagcgttttc gcttaagcga cggcttgtgc atggaagacg aacagttttc
    26101 cgtcaaacat tacgaagcgc gagtgaaaga cggcgtggtg cagctgcgcg gttaatgttt
    26161 taacgggagg cgcaatgcct cccctttttg catggtcctg taataacctt cggtatattg
    26221 caggacattt tttaaacttt ttgttttatt ttttgttttt attttttaaa ggataatcaa
    26281 atgtttacag acactattaa taagtgtgcg gctaacgctg cgcgcattgc acgcctgtcg
    26341 gcaaataacc cgctcggctt ttgggtcagc tccgccatgg cgggcgcgta tgtgggtctt
    26401 gggatcatcc tgatttttac gctcggtaat ttgctcgatc catccgtacg ccctctggtg
    26461 atgggcgcga cctttggtat cgccttaacg ctggtgatta tcgccggttc tgagctgttc
    26521 accggacaca ccatgttcct cacctttggg gtgaaagcgg gcaccatcag ccacgggcaa
    26581 atgtgggcaa tcctgccgca aacctggctg ggtaacctgg tcggttccgt cttcgttgcc
    26641 atgctctata gctggggtgg tggtagcctg ctgccggtag ataccagcat cgttcactcc
    26701 gtcgcgctgg ctaaaaccac tgcaccggca atggtactgt tcttcaaagg tgcattgtgt
    26761 aactggctgg tttgtctggc aatctggatg gcgctgcgca ctgaaggggc ggcgaaattt
    26821 atcgcaatct ggtggtgtct gctggcattt atcgcgtccg gctacgagca ctccatcgct
    26881 aacatgacgc tgttcgcgct ctcctggttc ggcaaccaca gcgaagccta cacgctggcg
    26941 ggtattggtc ataacctgct gtgggtgacg ctgggtaata ctttatcagg tgccgtattc
    27001 atgggattgg gttattggta tgctacgccg aaagcgaatc gtccggttgc ggacaaattt
    27061 aatcaaactg aaacggctgc cggttaatta ctaaggggtt tttacgtgga tcatttgcct
    27121 atattttgcc aattacgcga tcgcgactgt ctgattgtcg gcggtggtga tgtcgcggaa
    27181 cgcaaagcaa ggttgctgtt agacgcaggc gctcgcttaa cggtgaatgc attagcgttt
    27241 attccacagt tcaccgcatg ggcagatgca ggcatgttaa ccctcgtcga agggccattt
    27301 gatgaaagcc ttctcgacac ctgctggctg gcgattgcag cgacggatga tgacgcgctt
    27361 aaccagcgcg tcagcgaagc cgctgaagct cgtcgcatct tctgtaacgt ggtcgatgcg
    27421 ccgaaagctg ccagcttcat tatgccgtcg attattgacc gctcaccgct gatggtggcg
    27481 gtttcctctg gcggtacttc tccggttctg gcgcgcctgt tgcgcgaaaa actcgaatca
    27541 ctgctgccgc tgcatctggg ccaggtagcg aaatacgccg ggcagttacg cggtcgggta
    27601 aaacaacagt tcgccacgat gggtgaacgc cgccgcttct gggaaaaact gttcgttaac
    27661 gaccgtctgg cgcagtcgct ggcgaacaac gatcagaaag ccattactga aaccaccgaa
    27721 cagttaatca acgaaccgct cgaccatcgc ggtgaagtgg tgctggttgg tgcaggtccg
    27781 ggcgatgccg ggctgctgac actgaaaggt ctgcaacaaa ttcagcaggc agatgtggtg
    27841 gtctacgacc gtctggtttc tgacgatatt atgaatctgg tacgccgcga tgcggatcgt
    27901 gttttcgtcg gcaaacgcgc gggataccac tgcgtaccgc aggaggagat taaccagatc
    27961 ctgctgcggg aagcgcaaaa aggcaaacgc gtggtgcggc tgaaaggcgg cgatccgttt
    28021 atttttggcc gtggtggcga agagctggaa acactgtgca acgcgggtat tccgttctcg
    28081 gtggttccgg gtattaccgc agcttctggt tgctcagcct attcgggtat tccgctcacg
    28141 catcgcgatt atgcccagag cgtgcgctta attaccggac acttaaaaac cggtggcgag
    28201 ctggactggg aaaacctggc ggcagaaaaa cagacgctgg tgttctatat ggggctgaat
    28261 caggctgcga ctattcagca aaagctgatt gaacacggta tgcctggcga aatgccggta
    28321 gcaattgtcg aaaacggaac agcagtcacg cagcgcgtga ttgacggtac gctcacacaa
    28381 ctgggcgaac tggcgcagca aatgaatagt ccatcgctaa ttattattgg tcgggttgtt
    28441 ggcctgcgcg ataaactgaa ctggttctcc aaccattaat ttaatcaggg ccagagaatt
    28501 c
//
LOCUS       DBFIFM010000041        28204 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.041, whole genome shotgun sequence.
ACCESSION   DBFIFM010000041 DBFIFM010000000
VERSION     DBFIFM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.041"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(76..1341)
                     /locus_tag="N7C74_001569"
     CDS             complement(76..1341)
                     /locus_tag="N7C74_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2431464.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALVVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANRERFISPFEYAKINNPLKQ"
     gene            complement(1539..1614)
                     /locus_tag="N7C74_001570"
     tRNA            complement(1539..1614)
                     /locus_tag="N7C74_001570"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1579..1581),aa:Phe,seq:gaa)
     gene            complement(1720..2427)
                     /gene="yqgA"
                     /locus_tag="N7C74_001571"
     CDS             complement(1720..2427)
                     /gene="yqgA"
                     /locus_tag="N7C74_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HGU2431465.1"
                     /translation="MVIGPFINASAILLGGMLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2580..2663
                     /locus_tag="N7C74_001572"
     CDS             2580..2663
                     /locus_tag="N7C74_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2431466.1"
                     /translation="MHAISLHSLAYRRFARNPSLFMLLLMR"
     gene            2825..4960
                     /gene="speC"
                     /locus_tag="N7C74_001573"
     CDS             2825..4960
                     /gene="speC"
                     /locus_tag="N7C74_001573"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HGU2431467.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILTLLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGDVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(5010..6266)
                     /gene="nupG"
                     /locus_tag="N7C74_001574"
     CDS             complement(5010..6266)
                     /gene="nupG"
                     /locus_tag="N7C74_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HGU2431468.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6468..7547)
                     /gene="mltC"
                     /locus_tag="N7C74_001575"
     CDS             complement(6468..7547)
                     /gene="mltC"
                     /locus_tag="N7C74_001575"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HGU2431469.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGGTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7612..7887)
                     /gene="yggX"
                     /locus_tag="N7C74_001576"
     CDS             complement(7612..7887)
                     /gene="yggX"
                     /locus_tag="N7C74_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HGU2431470.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7915..8967)
                     /gene="mutY"
                     /locus_tag="N7C74_001577"
     CDS             complement(7915..8967)
                     /gene="mutY"
                     /locus_tag="N7C74_001577"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HGU2431471.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9128..9847
                     /gene="trmB"
                     /locus_tag="N7C74_001578"
     CDS             9128..9847
                     /gene="trmB"
                     /locus_tag="N7C74_001578"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HGU2431472.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMIEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9847..10173
                     /gene="yggL"
                     /locus_tag="N7C74_001579"
     CDS             9847..10173
                     /gene="yggL"
                     /locus_tag="N7C74_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HGU2431473.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERNLDEVRTSEL
                     FDVWWD"
     gene            10357..11076
                     /gene="yggN"
                     /locus_tag="N7C74_001580"
     CDS             10357..11076
                     /gene="yggN"
                     /locus_tag="N7C74_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HGU2431474.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11252..12298
                     /gene="ansB"
                     /locus_tag="N7C74_001581"
     CDS             11252..12298
                     /gene="ansB"
                     /locus_tag="N7C74_001581"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HGU2431475.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFIASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12415..13422
                     /gene="yggM"
                     /locus_tag="N7C74_001582"
     CDS             12415..13422
                     /gene="yggM"
                     /locus_tag="N7C74_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HGU2431476.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWTVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLETALN
                     IQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13665..14801)
                     /gene="hemW"
                     /locus_tag="N7C74_001583"
     CDS             complement(13665..14801)
                     /gene="hemW"
                     /locus_tag="N7C74_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HGU2431477.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGSAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEPAPRVEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(14794..15387)
                     /gene="rdgB"
                     /locus_tag="N7C74_001584"
     CDS             complement(14794..15387)
                     /gene="rdgB"
                     /locus_tag="N7C74_001584"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HGU2431478.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTELGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSACYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(15395..15685)
                     /gene="yggU"
                     /locus_tag="N7C74_001585"
     CDS             complement(15395..15685)
                     /gene="yggU"
                     /locus_tag="N7C74_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HGU2431479.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEVAALIN"
     gene            complement(15682..16248)
                     /gene="yggT"
                     /locus_tag="N7C74_001586"
     CDS             complement(15682..16248)
                     /gene="yggT"
                     /locus_tag="N7C74_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HGU2431480.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(16266..16970)
                     /gene="yggS"
                     /locus_tag="N7C74_001587"
     CDS             complement(16266..16970)
                     /gene="yggS"
                     /locus_tag="N7C74_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HGU2431481.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            16988..17968
                     /gene="yggR"
                     /locus_tag="N7C74_001588"
     CDS             16988..17968
                     /gene="yggR"
                     /locus_tag="N7C74_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HGU2431482.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLTENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGTPPILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(18159..18575)
                     /gene="ruvX"
                     /locus_tag="N7C74_001589"
     CDS             complement(18159..18575)
                     /gene="ruvX"
                     /locus_tag="N7C74_001589"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HGU2431483.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKIDSASAVIILESYFEQGY"
     gene            complement(18575..19138)
                     /gene="yqgE"
                     /locus_tag="N7C74_001590"
     CDS             complement(18575..19138)
                     /gene="yqgE"
                     /locus_tag="N7C74_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HGU2431484.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(19250..20197)
                     /gene="gshB"
                     /locus_tag="N7C74_001591"
     CDS             complement(19250..20197)
                     /gene="gshB"
                     /locus_tag="N7C74_001591"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HGU2431485.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQ"
     gene            complement(20210..20941)
                     /gene="rsmE"
                     /locus_tag="N7C74_001592"
     CDS             complement(20210..20941)
                     /gene="rsmE"
                     /locus_tag="N7C74_001592"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HGU2431486.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(21021..21728)
                     /gene="endA"
                     /locus_tag="N7C74_001593"
     CDS             complement(21021..21728)
                     /gene="endA"
                     /locus_tag="N7C74_001593"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HGU2431487.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDHYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(21823..22320)
                     /gene="yggI"
                     /locus_tag="N7C74_001594"
     CDS             complement(21823..22320)
                     /gene="yggI"
                     /locus_tag="N7C74_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HGU2431488.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYSCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(22397..23791)
                     /gene="galP"
                     /locus_tag="N7C74_001595"
     CDS             complement(22397..23791)
                     /gene="galP"
                     /locus_tag="N7C74_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HGU2431489.1"
                     /translation="MPDAKKQGQSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAGLNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(24228..25382)
                     /gene="metK"
                     /locus_tag="N7C74_001596"
     CDS             complement(24228..25382)
                     /gene="metK"
                     /locus_tag="N7C74_001596"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HGU2431490.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            25438..25689
                     /gene="yqgD"
                     /locus_tag="N7C74_001597"
     CDS             25438..25689
                     /gene="yqgD"
                     /locus_tag="N7C74_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HGU2431491.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            25800..25943
                     /gene="yqgG"
                     /locus_tag="N7C74_001598"
     CDS             25800..25943
                     /gene="yqgG"
                     /locus_tag="N7C74_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HGU2431492.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            26037..26168
                     /gene="yqgB"
                     /locus_tag="N7C74_001599"
     CDS             26037..26168
                     /gene="yqgB"
                     /locus_tag="N7C74_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HGU2431493.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            26177..28153
                     /gene="speA"
                     /locus_tag="N7C74_001600"
     CDS             26177..28153
                     /gene="speA"
                     /locus_tag="N7C74_001600"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HGU2431494.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
BASE COUNT         6897 a         7038 c         7420 g         6849 t
ORIGIN      
        1 ctaataccgt tttgtctgtt tttttaacaa agaaaataat cctggagtag gttcccgggc
       61 atttgcccgg gatgattact gttttaatgg attattaatc tttgcatatt caaatggact
      121 gataaatctt tctctgtttg catccagaaa atcggcccac cactgtagca tcagtcgccg
      181 ttcttccaga tgttctgctt tatgaatgta agcagcacgt acaccgttac gttcctgatg
      241 gctcatctga cgttcaactg catcgcggga ccacaaacct gattctatta acgcactaca
      301 tgccatcgcc cggaatccat gaccacagac atcctgggtt gtgtcatacc ccataaccct
      361 cagggcttta tttacggtgt tttcactgat tggtttacgt gggtcatgtg ctcccgtaaa
      421 gatcagacca ttttcaccag cccatgtctg aagttctgcc agtatggcta caacctgttt
      481 gctgagtggc acaagatgtg gtgttcgcat cttggatccc cggtgtgaga attttacgcc
      541 tggcagaggt tcgcgttcgg ctggaatagt ccacatggca ttgtcaatat caatttcaga
      601 ccagcgggcg tatctgagtt cactggaacg aataaaaacc agtaaattca gcataacggc
      661 aagctgggtg acaggttgcc ccttgtaact gccaatacgc gacaaaagtt caggcaggcg
      721 agaaagatca agagcggggc gatgctggcg ttttaccgta gtcaatgcgc cagccatatc
      781 cagagcagga ttatatctga tgatgccaga ctgtacggca taacgcatta cggcagatat
      841 tctttgctgc agacgactgg cgatttcata aatttgttta gcttctgcag cacgaacagg
      901 gataagcagg tccggggtat tgagtgttgt gatatcccga ttgccgataa aggggaatac
      961 gtgtgtctcg aggcttttga gtactttatc gctgtgtgat tgtgcccatt gtttattact
     1021 ggcatgccag cgtcgtgcaa tatgttcgaa agagagttgt aaatcaggga ctactttttt
     1081 agctcttttc tgttcgcaag ggtcaacccc ctccgaaatc agttttcggg cctcatcccg
     1141 ttttgtacga gcttcggaga gagatacttc tggatacaca acaagtgcca gagtcttctc
     1201 tttacccaga atacgataac ggagacgcca gtaacgggaa ccattaggat gtaccagcag
     1261 gaacatgcca gccccgtcag tgagtttata agccttgtca gtgggctttg cagcccggat
     1321 ttttgcgtca gtcagtgcca tagttcagcc ttctgaatgc gttaaagagg gtacaaattc
     1381 tgtcgaactg gggtatgccc gcaattatac ccacacaatg aggttgatgt ggggagaatt
     1441 tggttgagtt cggtagaatt gatttggagg aagaacactt aaatcatggt gttctgaaac
     1501 gaaaaacgga cctccgtgga ggtccgttga tatgaatttg gtgcccggac tcggaatcga
     1561 accaaggaca cggggatttt caatcccctg ctctaccgac tgagctatcc gggcaacggg
     1621 gcgcattaaa cctgattcgc ctcgtctcgt caatcaaatt tcttcaattt actgcagact
     1681 gcacaatcta tcatcacttt gccgctattg cacacattgt caggcaaacc aggcggtcca
     1741 ggcggcggaa agcggcattg ccagaaggag tgctggaagc atgttgacca ccgggaacat
     1801 tttaatgcca cagatgcgta atccggttgc cagcagcaat aaaccgccta cggcgctgaa
     1861 gtctgccatc atcgacggtg tggtcagcgg taatatcagc gcggcagccc acgccagcgt
     1921 taactggatg atcagtaatg ggatactaat caccgatacc gcaataccca gcgagcaggc
     1981 gaagatcatc gccgtaaaga aatcaagaaa tgacttggcg attaaaatac tcggatcgcc
     2041 ggtcatccct tcgttcatcg ccccgaagat cccggtgccg ctggcgcaaa acaggacaat
     2101 aatcgcgaca taattctgaa taaaagattc atgcgctggc ttcttacgtg agtgacgaaa
     2161 cagattttgt gctttggcga ccgctgtatt gacgcctttc tccagcagac aaatttcgcc
     2221 gattaatgct ccgagtaagg ttgctaaaac catcgccgga aggttggcgc atttcaccac
     2281 cagtaaaata ccaatcccca gcgatgccaa accaaaaatt gatgtcatgg agacgcggat
     2341 acgttccggt aagcgttggc taagcagtgc accgagcatg ccacccagta atattgcact
     2401 tgcgttgata aaagggccga tgaccacaag agttcctgtt aactgtattg tttgattttc
     2461 ttattatgaa ctttttgcct tgttgatggc ttgtctggtc gcaaagtcct ggcttgcacg
     2521 ccttagcgaa aggtgccatg attgcgcgaa ttttctcctc tctgtacgga gtttgcccga
     2581 tgcacgccat ctccttacat tctctcgctt atcgccgttt cgcgcgaaac ccttctcttt
     2641 ttatgctact gctcatgcgg tgaagttaac gcacgctcac tgcaggacaa cagtaaaatc
     2701 agagcctttc tgcttttact gatgtctggc ggtcggagct ggtgaccagt ttgacccata
     2761 tctcatgggg cagggtttta caccttgtcc agtattctta cttccccgaa acgggtttgc
     2821 gcttatgaaa tcaatgaata ttgccgccag tagtgaactg gtatcccgac tttctactca
     2881 tcgccgcgtg gtggcgttgg gagatactga ttttacggac gtcgcggcag tcgtcattac
     2941 cgctgcggat agtcgcagtg gcattcttac gttgcttaag cgcaccggtt ttcatctacc
     3001 ggtgtttttg tattccgaac atgctgttga attacctgcg ggcgttacgg cggtaatcaa
     3061 cggcaacgag cagcagtggc tggagctgga atccgcagcc tgtcagtatg aagagaattt
     3121 gctgccaccg ttttatgaca cgctgacgca gtacgttgag atgggcaaca gcacctttgc
     3181 ttgccctgga catcaacatg gtgcgttctt taaaaagcat cctgccggac gccattttta
     3241 cgatttcttt ggtgagaaca tctttcgcgc cgatatgtgt aacgctgacg taaaattggg
     3301 cgatctgctt attcatgaag gatcggcgaa agatgcgcag aaattcgcag ccaaagtctt
     3361 tcatgccgat aaaacctatt ttgtgctgaa cggcacatcg gcagcgaata aagtggtgac
     3421 gaatgcgctg ttaacgcgtg gcgatctggt gctctttgac cgtaacaacc ataagtcgaa
     3481 tcatcacggc gcgttgattc aggcgggggc gacgccggtc tatctggaag cttcacgtaa
     3541 cccgtttggt ttcattggcg gtattgatgc gcactgtttt aatgaagagt atctgcgcca
     3601 gcaaattcgc gacgttgcgc cagaaaaagc cgacctgccg cgcccgtttc gcctggcgat
     3661 tattcagctg ggaacctatg acggcactgt ctataacgcc cgtcaggtga tcgataccgt
     3721 tgggcatctg tgtgattaca ttctgtttga ttccgcgtgg gtcggttatg aacaatttat
     3781 cccgatgatg gcggatagct cgccgctgct gttagaactt aacgaaaacg atccggggat
     3841 ctttgtgact cagtcggtgc acaaacagca ggcgggattc tcacagacgt cgcagatcca
     3901 taaaaaagat aaccatatcc gcggacaggc gcgtttttgc ccgcataagc ggttgaataa
     3961 cgcctttatg ctccatgctt ctaccagccc tttctatccg ctgtttgctg cactggatgt
     4021 taacgccaaa attcatgaag gggagagtgg gcgtcggctg tgggctgagt gcgttgagtt
     4081 gggtattgag tcgcgcaagg ctattcttgc gcgctgtaag ctgttccgcc cgtttatccc
     4141 gcccgttgtt gatggcaaat tgtggcagga ttatccgaca tcagtgttag ccagcgaccg
     4201 tcgttttttc agttttgagc cgggggcgaa gtggcacggc tttgaaggat atgccgcgga
     4261 tcagtatttt gttgatccgt gcaagctgtt actcactaca ccaggtatcg atgccgaaac
     4321 cggcgaatat agcgactttg gcgttccggc gacgattctg gcgcactatc tgcgtgagaa
     4381 cggcattgtg ccggagaagt gcgatctcaa ttccattctg ttcttattaa ctccggcgga
     4441 aagccacgag aagctggcac aactggtggc gatgctggct caatttgaac agcatattga
     4501 ggatgactcg ccgctggctg aggtgttgcc gagcgtttat aacaaatatc cggtgcgcta
     4561 tcgcgactac accctgcgcc agttgtgtca ggagatgcac gatctgtatg tcagtttcga
     4621 cgtcaaagac ctacaaaaag cgatgttccg ccagcagagt ttcccgtcag tggtgatgaa
     4681 cccccaggat gcgcatagcg cttatattcg cggtgacgtg gagttggtgc ggattcgtga
     4741 tgccgaaggg cgaattgcgg cagaaggggc gttgccttat ccacctggcg tgctttgcgt
     4801 ggtacccggg gaagtctggg gtggggcggt tcaacgttat ttccttgcac tggaagaagg
     4861 ggtgaatttg ttgccgggat tttcgccgga gctgcaaggt gtttatagcg aaaccgatgc
     4921 ggatggcatg aaacggttgt acggttatgt gttgaagtaa gaataaaaaa aacgggtcgc
     4981 cttctggcga cccgtttttc tttgcgtaat tagtggctaa ccgtctgtgt acctgtcggg
     5041 acacgaacgt gtttatattt gaacatcgcc atgaacgcga aggccagaac cacggagtaa
     5101 ccagcgaaaa tcaaccatac ggtctgccag tcggtaatac cgttttgggt gtacatctca
     5161 acaactttac cgctcacgat gccgccgagg atacagccga agccgttagt catcatcagg
     5221 aacatccctt gtgcactggc gcgaattgcc gggctaactt ctttttcgac aaacaccgaa
     5281 ccagagatgt tgaagaagtc gaacgcacag ccgtaaacaa tcatcgatag aaccagcagt
     5341 acggtgccga acggagtcgg gtcgccgtaa gcaaacagcg caaaacgcag gatccacgcc
     5401 acaatactga tcatcattac gttcttaata ccgtagcggc ttaagaagaa cgggatggtc
     5461 agaatgaaca gggtttcaga gatctgcgaa atcgacatga tgattgacgc atgctgcaca
     5521 ataaagctgc tggcaaacat cggatctttg tcgaagctgt gcaggaaggt attaccgaac
     5581 atgttggtaa tctgcagttc cgcgcccagc agcattgaga agatgaagaa gattgccata
     5641 cgcttgtttt taaacagcgc gaatgcatcg aggcccagca gggttgtcca gctctgattc
     5701 gcttgctgtt tagcaaccgg aatatgcggc agagtcaggg taaacagaac cagaatggcg
     5761 gaaagtgctg cgccaatata cagctgcatg tggcttaatt cgaagccaga caggctcacc
     5821 acccacattg ccatgataaa gccgatggtg ccccagatac ggattggcgg gaagtcagta
     5881 acgatatcca tcccggcatt ttgcaggcga tagtaagaga tggtgttgat taacccaagc
     5941 gttggcatat aagcaaacga gttaatcaat atcacaagga acatcgcctc cggcgtcgtg
     6001 acctgtgccg ccatgaacag cgtgatagcg ccaatggtgt ggcaaatggc atatacccat
     6061 ttcgcactta accatttgtc ggccacaatc cccagcagcg caggcataaa gaccgcggcg
     6121 atacccagtg agctataaac tgcgccaata gaagcaccgt caaacttcag ggtaacaaac
     6181 atataggaac cgagggtcgt cagccaactt ccccacagac agaactgcag aaaagagagg
     6241 attttcagct gcagcttaag attcatgtta atttcctcac atcgtgatgc ggatgagtgt
     6301 ttgaaaaagc acatttgacc gtgcgagatg atgggattct atcaacttcg cggactggtt
     6361 ttttgttacc tgtggcaaat aattgcaaac attcctaatt tgcaaaacga aattgtgata
     6421 tggataacat ttttgcccct gagcatcgtc aggggcggtt agtggaatta tcggcggcgg
     6481 taggattttt gcgcggtatt cactttataa agataacggc gagattccgc agagggatgg
     6541 cgggtcgtca gcgtttgata aacatcgccc ggcgtcatgg tgttaataat attagccgcc
     6601 tgaatcttat cattcgaaaa gactcgcagc acgctgcctg cgccgccgtt ataggcggtg
     6661 atgacggcat aacgccggga tgtagggtta tcaattccgc cgagataaac attgttcagc
     6721 atcgccagat acgcggtgcc ggtatcaata ttgctggcag gatcaaacaa gaaactgcgg
     6781 ctcggcgtgc cggatttccc ctgcgagcgg aacacatctt tcccggcagt atgttgtacc
     6841 acctgcatta accccagcgc atcggaacgg ctgaccgcat acgggttaaa ggaagattca
     6901 gtctgcataa ttgccagaat cagcgactca tcaacgccat atttccgtga cgcctggcgg
     6961 accatgccga gatatttgtg cgcacgttta tcaaggtggt tcggcaccat gttaatggtg
     7021 acgctgtaga tgatacgcag cccgttgctg cggctcttca gacggttttt cagcagataa
     7081 tccgcgaagt tgcttgcgcg accttcccag cgaatcggct gcccggtgtt gtccaccacc
     7141 tgaccgtaaa ggaaaggttc tttcgaaatt gtaatatcat caacgtcgga atagagatcg
     7201 accgaactcg gatcgtcacc catcagcaac gttttgataa ttgcccggcg caaatgcgcg
     7261 gcaggttctg tcccggcgat ggtttcgata gtaatcgtac cgtcatcgaa gttgatgtgg
     7321 ctgcgggtct gatattgatc ggtgtatttc acgtagtcct taggaccggc gatcaccacc
     7381 tctttgaatc cccagatgtt ctcaatattg tgggcaaatt gccccatcag aatatcaaaa
     7441 ccgttggtgt ctttgaccca ggcttcgtta taggtaccgc cttttttggt cgtcgaacag
     7501 gagatgagca acggcgcaat caaagccagc gcgagatatt ttttcatcat tccgggtgcg
     7561 tgttgtgttt gtttttatgc aagctgccgg aggcgtgctc cggcactgtt tttatttttt
     7621 atcttccggc gtatagccct cgatatgcac ctctttaccc tcgaacagga agttgaccat
     7681 ctcctgctcc agcagcttgc ggtgctcggc attcatcatg ttgagtttct tttcattaat
     7741 cagcatggtt tgtttgtgct gccactgcgc ccaggcttct ttggagatct cgttatagat
     7801 gcgttttccc agttcgccgg ggtacagctg aaaatcctga ccttctgctt cacgttgcag
     7861 gaaagtacaa aaaatcgttc tgctcataaa tcatcctctt tatcgactca cgcgctaaac
     7921 cggcgcgcca gtgcgtaact gctgtaacaa acgctccacg ggagccgcca ggccaactga
     7981 cggcggttgc gctaagttat accagagcgc attgccttca tccatgcagc cggtgaatga
     8041 cgacacggga agccacatag gcacaatatc taagtggaaa tggctgaagg tatgccgaaa
     8101 cgcggtcagc tgcgtcaggt tatcggcagc aatctgccgt tgcgccagcc actgccgcaa
     8161 actttcttcg tcggcaaact gcgggaaaca gtataaaccg ccccacaatc cgctcggcgg
     8221 acgctgcgcc agcaatactt catcttcgtg ctgtaacagg agaaagtagc cggtgcgctc
     8281 cggcagcgtc tgtttcggtt ttttgcccgg ataaagcgac cagctattgt tggtggcggc
     8341 aatacatccg ttttgtagcg gacagagcga acatttcggc ttcgaacgcg tgcaaatcat
     8401 cgcgcccaaa tccatcatcg cctgattaaa ccgttccacg ccgaccgcgg gcgtcacctg
     8461 ctcgcttaaa ctccataatt tattctcgac ctcttttttc ccaggccagc cgcttacagc
     8521 atagcagcgc gccagcaccc gtttgacgtt accgtcgaga atcggaaagt gcttacccag
     8581 agaaagcgat agaatcgcgc ctgcggtgga acgcccgacg cccggcagtg ctgcaacttc
     8641 ctcaaaggtt tccgggaatt taccgccgtg taaggtcgcc acttgttgtg ccgctttatg
     8701 cagattgcgc gcgcgggcgt aatagccaag ccccgtccac aagtggagaa cttcgtcgag
     8761 cggcgcattg gcgagatcgg tcaccgtcgg gaagcgcgcc ataaagcgtt caaaataggg
     8821 gataacggtc gcaacctgag tttgttgcaa catcacttct gagagccata ctttgtaggg
     8881 cgtcttgtca atttgccagg gcagagtttt tcgcccgtat ttatcgtacc agtccagaac
     8941 ctgggctgaa aattgcgacg cttgcatggt caccgaattc actgttgttg ggggcaagat
     9001 tgcagcacag cgacggcagg gtgtaaaccg gaactttccg cagccacggc cttatcatgc
     9061 ttgcatccgg caactaactt tggataatgc ccgttttcag aacactttca caagcgacta
     9121 aatctttatg aaaaacgacg tcatttcacc ggaatttgat gaaaacggcc gcccactgcg
     9181 ccgtatccgt agttttgtgc gccgccaggg gcgactgacc aaaggccagg aacatgcgct
     9241 ggaaaactac tggccggtga tgggcgttga gttcagcgaa gatatgctgg atttccccgc
     9301 gctttttggc cgtgaagcgc cggtgacgct tgagattggt tttggcatgg gggcgtcgct
     9361 ggtggcaatg gctaaagatc gccccgagca ggacttcctc ggcattgaag tgcattcacc
     9421 gggcgttggt gcgtgcctgg cttctgcgca tgaagagggc ttaagcaacc tgcgcgtgat
     9481 gtgtcacgat gcggttgaag tgctgcataa aatgattcct gacaattcat tgcgcatggt
     9541 gcagctcttt ttccctgacc cgtggcacaa agcgcgccat aataaacgcc gtatcgttca
     9601 ggtgccgttt gccgaactgg taaaaagcaa actgcagctg gggggcgtat tccatatggc
     9661 gaccgactgg gaaccttatg cggaacatat gattgaagtg atgtcttcta ttgacggtta
     9721 taaaaacctg tcagagagca atgattacgt accgcgtccg gcatcacgtc cggtgacgaa
     9781 atttgaacaa cgtggtcatc gtcttggtca cggagtatgg gacttaatgt tcgagagggt
     9841 gaaataatgg caaagaaccg tagccgtcgt ctgcgtaaaa aaatgcacat cgacgaattc
     9901 caggaattag gattttcggt ggcatggcga ttcccggaag gtacatcgga agaacagatt
     9961 gataaaaccg ttgatgattt tattaacgag gttatcgaac cgaacaaact ggcctttgac
    10021 ggcagcggtt atctggcctg ggaaggtctg atctgcatgc aggaaatcgg caaatgcacc
    10081 gaagaacatc aggcgattgt gcgtaagtgg ctggaagagc gcaacctgga tgaggtacgc
    10141 accagcgaac ttttcgacgt ttggtgggac taagaaagca tacgggcgat gacaaatgca
    10201 aaactgcctg atgcgctacg cttatcatgc ctacaaagat gcacgatcga gtaggcggga
    10261 taaggcgttt acgccgcatc cggcatggaa aacgcgtact ttgttatcaa tctggggcca
    10321 gcaaatgctg gcctgatttg ttcttgaggg aagactatga tgcgcaaaat gctgctggcg
    10381 gcagcacttt cagtgacggc aatgaccgct cacgccgact accagtgcag cgtcacgccg
    10441 cgtgacgatg tgattgtcag cccgcaaacc gtgcaggtga agggcgaaaa cggcaatctg
    10501 gtgatcacgc cagacggcaa cgtgatgtat aacggtaagc aatattccct gaatgccgcc
    10561 cagcgcgagc aggcgaagga ttatcaggct gaactacgta gcacgctgcc gtggattgat
    10621 gaaggcgcga aaagccgcgt cgagaaagcc cgtattgctc tggataaaat tatcgttcag
    10681 gagatgggcg aaagcagcaa aatgcgcagc cgtctgacca aacttgatgc gcagctgaaa
    10741 gagcagatga accgcattat cgaaacgcgc agcgatggcc tgacgttcca ctataaagcc
    10801 attgatcagg ttcgcgctga aggccagcaa ttagtgaatc aggcaatggg cggaatttta
    10861 caggacagca ttaatgaaat gggcgcgaaa gcggtgctga aaagcggcgg taacccatta
    10921 cagaatgtgc tgggaagcct gggggggctg caatcctcaa tccaaaccga gtggaaaaag
    10981 caggaaaaag atttccagca gtttggcaaa gatgtttgta gccgcgttgt gactctggaa
    11041 gatagccgca aagccctggt cgggaattta aaataatcct ctattttaag acggcataat
    11101 acttttttat gccgtttaat tcttcgtttt gttacctgcc tctaactttg tagatctcca
    11161 aaatatattc acgttgtaaa ttgtttaacg tcaaatttcc catacagagc taagggataa
    11221 tgcgtagcgt tcacgtaact ggaggaatga aatggagttt ttcaaaaaga cggcacttgc
    11281 cgcactggtt atgggtttta gtggtgcagc attggcatta cccaatatca ccattttagc
    11341 aaccggcggg accattgccg gtggtggtga ctccgcaacc aaatctaact acacagcggg
    11401 taaagttggc gtagaaaatc tggttaatgc ggtgccgcaa ctgaaggaca ttgcgaacgt
    11461 taaaggcgag caggtagtga atatcggctc ccaggacatg aacgatgatg tctggctgac
    11521 actggcgaaa aaaattaaca ccgactgcga taaaaccgac ggcttcgtca ttactcacgg
    11581 taccgacacg atggaagaaa ccgcttactt cctcgacctg acggtgaaat gcgacaaacc
    11641 ggtggtgatg gtcggcgcaa tgcgtccgtc cacgtctatg agcgcagacg gtccattcaa
    11701 cctgtataac gcggtagtga ccgcagctga taaagcctcc gctaatcgtg gcgtgctggt
    11761 ggtgatgaac gacaccgtac tggacggtcg cgatgtcacc aaaaccaaca ccaccgacgt
    11821 agcgaccttc aagtctgtta actacggtcc gctgggatac attcacaacg gtaagattga
    11881 ctaccaacgt accccggcac gtaagcacac cagcgacacg ccgttcgatg tctctaagct
    11941 gaatgaactg ccgaaagtcg gcattgttta taactacgct aacgcatccg atcttccggc
    12001 taaagccctg gtagatgcgg gctatgatgg catcgtgagc gctggtgtgg gtaacggcaa
    12061 cctgtataaa tccgtgtttg acacgctggc gaccgccgcg aaaaacggta ctgcagtcgt
    12121 gcgttcttcc cgcgtaccga cgggcgctac cactcaggat gccgaagtgg atgatgcgaa
    12181 atacggtttt attgcctctg gtacgctgaa cccgcaaaaa gcgcgcgtcc tgctgcaact
    12241 ggctctgacg caaaccaaag atccgcagca gatccagcag atcttcaatc agtactaatc
    12301 atttcgcccc ggtatcgcgc cggggctttt tcacttcaga cttacgtcca ttgccaattt
    12361 taattgccct aatgataatc accagaataa attattccgc gcgagggttt tcgggtgaaa
    12421 aagcaatgga ttgttggcac ggcgctgctt atgttgatga ctggtaatgt ctgggcagat
    12481 ggcgaaccgc caactgaaaa tatcttaaaa gatcaattca aaaagcagta tcacggcatt
    12541 ctcaagcttg atgccatcac cttaaaaaat cttgatgcta agggtaatca ggccacctgg
    12601 tcagcggaag gcgatgtctc ttccagtgac gatctctata cctgggtcgg tcagttggca
    12661 gattacgaac tgctcgaaca gacctggacg aaagataaac cggtaaaatt ctcggcgatg
    12721 ttaaccagta aaggaacgcc cgcgtctggc tggacggtga acttttactc ttttcaggcg
    12781 gcagctagcg atcgtggtcg ggtggtcgac gatatcaaaa cgaataataa atatctgatc
    12841 gtgaatagcg aagatttcaa ttatcgcttt agtcagcttg agactgcgtt gaatatccag
    12901 aagaactcga ttcctgcgct ggaaaaggaa gtgaaagcgc ttgataagca aatggtggcg
    12961 gcgcagaaag cagcggatgc atattgggga aaagatgcaa atggtaagca aatgacccgc
    13021 gaagatgcct ttaaaaaaat tcaccaacag cgtgatgagt ttaataaaca gaacgatagc
    13081 gaggcgttcg cggttaaata tgacaaagag gtttatcaac cggcgattgc ggcatgtcat
    13141 aaacagagtg aagagtgtta tgaagtgccg attcagcaga aacgagattt cgatatcaac
    13201 gaacaacggc gacagacttt tctgcaatca caaaaactta gccgtaaatt gcaggatgac
    13261 tggataacgc tcgaaaaagg gcaatatccg ttaacgatga aagtttcaga aataaacagt
    13321 aaaaaagtcg cgattttgat gaaaattgac gatatcaacc aggctaatga acgctggaaa
    13381 aaagataccg aacaactgcg gcgcaatggg gtgattaagt aagttttgat atagcgcacg
    13441 atttgtagca ggatgcggcg ttgaccccgc atccggcaat caacgtctga tgcgacgcta
    13501 gcgcgtctta tcagacctac aacagctgcc acatgtcggc tgaggtgctc acgccgcatc
    13561 cggcaatcaa cgtctgatgc gacgcttgcg cgtcttatca gacctacaac cgttgccaca
    13621 tgtaggctgg ataaggcgtt cacgccgcat ccggcaatac aagtttactc agccagaaaa
    13681 agctccagca gcgaatttaa aaacagcttc ccatgttccg taatctgcca gtaatccgca
    13741 cattcggtga gataaccctg ggcaatcgcc tcgtctaact gtgggcgaat cacatcttcg
    13801 caaagcccgg tatacgcact aaactccacg cgcggcgcag gttccagcaa ccggaagcga
    13861 ttcataaaga actcaaacgg cttatctgcg gcttcgacat cacgctggct ttccagatac
    13921 cttccttgca taaaaccacg cggatgacgc gttttggtgg tacgcagaat gcgcccatcc
    13981 gggaaggtca ctttgccgtg cgcgccgcag ccaataccga tgtagtcacc aaagcgccag
    14041 tagttgagat tgtgctggca ctgataaccg ggtttggcgt aagcggaagt ttcatattgc
    14101 tgataacccg ctgcggttaa taactgatgc ccctgttcga aaatatccca cagcgcgtcg
    14161 tcgtccggca gtaccggcgg gcgtgaacca aacagcgtat taggttcgat ggtcagttga
    14221 taccaggaaa gatgcggcgg attcagttca atagcctggc gcagatcgcc aagcgcctct
    14281 tccagcgatt gatccggtag cccatgcatc aaatcaaggt taaagctacg taaccctaaa
    14341 ccgctcgcca gctttgccgc tcgtttcgct tcttgcgggc catgaatacg tcccagccgt
    14401 ttcagctttt cttcgctaaa actctgtacc ccaatagaga tacgatttac accggcacgc
    14461 tgataatcaa caaagcgatc ggcttctacc gtaccggggt tcgcttccat ggtaatttct
    14521 gcatccgctg ccagcggcaa acgcgcacgc acgccgtcca gcagcgtttg catcgccgag
    14581 ccggaaagca ggctcggcgt accaccgcca ataaagattg tctttacttc acggccctga
    14641 gcgtaagcca catcgttgtc cagatcgtta agcagatgct gaacgtaatc gtcgtgcggc
    14701 acttctcctt tcaacgcgtg agagttgaaa tcgcagtacg ggcatttctg cacgcaccac
    14761 gggatgtgaa tgtagagact cagcggcggt aatttaacca ttacgtaaag cgtccagcag
    14821 cagtttcaat gcctgaccac ggtgggaaat ggcgctcttt tcttcgcggg tcagttcggc
    14881 agcggttttc ccttcggaag gtacgaagaa gattggatca taaccaaagc caccagtgcc
    14941 cgccggttcg cgagtaatca cacccggcca gctgccatgg cacaccagcg gggtcggatc
    15001 ttctgcgtga cgcagataca ccagcacgca gtggaaacgc gcctgacgtt ggtcgtccgg
    15061 tacgtctttc agtgtttcca gcagtttttg cagattcttt tgatcggtcg cgtcttcacc
    15121 ggaataacat gcggagtaaa tacccggcgc gccgccaagc acatctaccg ccaggccaga
    15181 gtcgtcggca attgccggta aaccggtcac ttttgccgcg tggcgcgctt tcagaatcgc
    15241 gttttcgata aaggtcaggc cggtttcttc agcggaatca acgccgagtt ctgtttgggc
    15301 cacgatatca agaccgaagt cgctaagcag cgacgccagc tcacgcactt taccggcatt
    15361 gccggttgcg aggacaactt tttgcatagg atacctaatt aattaacgcc gcgacttctg
    15421 gcgggatttg ttgcggatta atgattttaa tttgtttgtg gcggccaagt tcgccttttt
    15481 caatcaccac ctggcttttg gcaacccgga attgcttacc gagaaacttc accagatggc
    15541 tgttggcctg accgtcaacc ggcggcgcgg taatggcgac ttttacctcg tcgccatgta
    15601 aaccgacaat agaatcacgg ctggctttcg gctgaatata gagccgtaaa accagaccgt
    15661 catcgttaac tgttacggca ctcataacgc catccacagc cccggcagca gcatatttcc
    15721 ggtcgcctgt aatacttctg cgacacccat attgatgaca tacagcagca gaacgaggat
    15781 catcggcgag aaatcaatcc cccccattgc cggtagcaag cggcgaatcg ggcgcagcag
    15841 cggatcggcc agctgaatca gcacgtattc aatcgggcta cggccctggc ttacccagct
    15901 cataatcgcc atcaccagca ggacccagaa aatcagcagg ccgatggttt tcagcagaat
    15961 cagtaaaccg gcaatccaga tgattggcag gaaggtcacc actttaaaca gcacgatggc
    16021 tttgataaaa ctgagaatat aggcaaccag cagcgaggcg ctatcaatgg gtcccattgc
    16081 cggaataacg cggcgcagtg gcccgataat tggctgcgtt accttcacta caaactgtga
    16141 gaaggggttg taaaaatcac aatgagccca ctgcatccag atgcgtaata acagcaccat
    16201 ggtatacagc tcaatgaccg ttgaaagcag gaaagtcaac gtattcatgg cgttccttta
    16261 attccttatt ttttagagta atcacgcgca ccaaaaattg cagtgccgat acgaaccatc
    16321 gtgctacctg ccgcaatggc ggcttccata tcgtccgaca ttcccagaga gagcgtgtcg
    16381 atatgcgggt agcgcgtttt cagtccggca aatgctacag ccatttggcg tgcaacttca
    16441 aactgcctta catattctga ctcaggcgca gggattgcca tcagcccgcg cagacgtaaa
    16501 cgcggtagtt cagcgaccgc agctgccagc tcgtccagtt ctgccagttg aatcccggac
    16561 ttactgtttt catcactaat attaatttga atcagtacgt taagaggggg aagttctgcc
    16621 gggcgctgat cgttgaggcg ggtagcaatg cgcaaacggt cgatggtatg acaccagtcg
    16681 aaatgctctg ccaccaggcg gcttttatta gactgcaacg ggccaataaa atgccattct
    16741 aatcctgtta cgcccagttc ctgaaagtgg cgaattttat ctaccccttc ctgaacgtag
    16801 ttttcaccaa attgacgctg cccggcatca atggcttctg cgatggcgct cgcaggtttt
    16861 gttttactga ctgcaagcag cgtaatttct tctggagaac ggccgcaacg cgttgcagcc
    16921 gctgagattt tgtcccggac ctgtgccagg ttatgcgcaa tatcgttcat tttccgagga
    16981 tcttagtatg aatatggaag aaattgtggc ccttagtgta aagcataacg tctcggatct
    17041 acacctgtgc agcgcctggc ccgcacgatg gcgcattcgc gggctaatgg aagctgcgcc
    17101 gtttgatgcg ccggacgtcg aagagctact gcgggagtgg ctggatgacg atcagcgggc
    17161 aatattgctg gagaatggtc agctggattt tgccgtgtcg ctgacggaaa accagcgatt
    17221 gcgtggcagc gcattcgcgc aacggcatgg catttcgctg gcgttacggc tgttaccttc
    17281 gcactgcccg cagctcgaac agcttggcac accaccgata ttgccggagt tactcaagag
    17341 cgagaatggc ttgattctgg tgacgggggc gacggggagt ggcaaatcta ccacgctggc
    17401 ggcgatggtt ggttatctca atcaacatgc tgatgcgcat attctgacgc tggaagatcc
    17461 tgtggaatat ctctatgcca gccagcgatg tttgatccag cagcgggaaa tcggtttgca
    17521 ttgtatgacg ttcgcatcgg gattgcgggc cgcattgcgg gaagatcctg atgtgatttt
    17581 gctcggagag ctgcgtgaca gcgagacaat ccgtctggcg ctgacggcgg cagaaaccgg
    17641 gcatctggtg ctggcaacct tacatacacg tggtgccgcg caggcagttg agcgacttgt
    17701 ggattcattt ccggcgcagg aaaaagaccc cgtgcgtaat caactggcag gtagtttacg
    17761 ggcagtgctg tcacaaaagc tggaagtgga taaacaggaa ggacgcgtgg cgctatttga
    17821 attgctgatt aacacacctg cggtggggaa tttgattcgc gaagggaaaa cccaccagtt
    17881 accgcatgtt attcaaactg ggcaacaggt ggggatgata acgtttcagc agagttatca
    17941 gcagcgggtg ggggaagggc gtttgtgacg ttgccatgcc cgatgcgcaa gcttatcggg
    18001 cctacaaagg caataaacgt ctgatgcgac gcttgcgcgt cttatcagac ctacaaccgc
    18061 tgccacatgt aggccggata aggcgttcac gccgcatccg gcaataaggc gtaaggcgcg
    18121 atgatattat ttacactcca gcaggcgatt aaatctcctt aatatccctg ctcgaaatag
    18181 ctttcgagaa taataaccgc agaggcagag tcgattttgc ctttgttgag cgcccgatag
    18241 ccgccctgtt caaacagacc ggaacgtgcc tccacggtgc taagacgctc gtcatggagc
    18301 tttacttcaa caccgaaacg accatgaata cggttagcaa atttacgcgc tctggcggtt
    18361 aacggctgtt cggtgccgtc catattcagc ggtaaaccga cgatgatttc gtctggctgc
    18421 cactctttca gtaaacgttc gatgaggttc cagtccggcg taccgtcctg tgctttaatt
    18481 gcaggcaaag ggcgagcggt gccggtaatg cgttggccga ccgctacgcc aatgcttttg
    18541 gtgccgaagt cgaaggcgag taaggttcca ctcatcaggc gtgtcctgcc acaccaggca
    18601 tggtgagaat atccacacca atcagttttg ccgcctcgcg ccagcggtcg gcaattggcg
    18661 ttttgaacag aatattcaga tctgccgggg cggttagcca cgcgttatcg agaatttctt
    18721 gttccagttg acctttttcc caggaggcat agcccagagc caccaatacg tcagacggtt
    18781 gtttatcggt gccgagcgtt tccagcacat cgcgggaagt ggtcatcact gtgttgtctg
    18841 aaatgcgaat gctggaagca aaattggagg gcggagtatg caaaataaac ccgcgatctt
    18901 cagccagtgg gccgccgagc ataaccggtt tatccagacg gattgattca tcacgcggct
    18961 ccggcgtgat cttcagcttt tccagaatcc cttcaatttt gagattttcc agcggcttgt
    19021 tgacgatgat ccccattgca ccattggtat tatgttcgca aatgtagacc acggaacgac
    19081 ggaaaatcgg atcctggaga gcaggcatgg caataagaaa gtgatgctgt aaattcattg
    19141 tcagaggttc tgtttcctgg ttcaaaaaag caacagcgcc cagtatgcgt ggaaagaagg
    19201 gcgctgtcac tcagagtctc aacgagatcc ttctcgctaa ggtgggttac tactgctgta
    19261 aacgtgcttc gatggcatcc attaacattc cggtgatcga caccggaaac tctgcttcaa
    19321 tctcacgaat acaggttggg ctggtgacgt taatttcagt cagacggtcg ccgatgatat
    19381 ccagaccaac aaaaatcagc cctttttctt tcagcgtcgg cccgatctga cgggcgattt
    19441 tccagtcact ttccgtcagc ggacgaggtt caccgcgacc accggcagcc agattgccac
    19501 gggtttcgcc cccctgcgga atacgcgcca ggcagtacgg tactggttcg ccgtccacca
    19561 ccagcacgcg tttgtcgcca tctttaatgg ctggcagata attttgtgcc atgcagtagc
    19621 gagtgccgtg ttcggtcaag gtttcggcaa tgacgccgag gtttggatcg ccttctttca
    19681 cgcggaaaat cgacgcgccg cccataccgt ccagcggctt aagaatgatg tcgctgtgtt
    19741 tctcccagaa cgcttttagc tgcgctttat tgcgcgtaac cagcgtttct ggcgttaagt
    19801 cagagaacca ggcggtaaac agtttctcgt tacagtcgcg caggctctgc ggcttgttaa
    19861 cgatcagcgt ccctttctct tcggcacgtt ccagaatata ggtcgcgtag ataaactcgg
    19921 tatcaaacgg cgggtcttta cgcatcagga tcacatcgag atcggccagc ggcagatcct
    19981 gttcaccgac gaatgaaaac cactcttcgt agttctgttt cacgttcagc gtgcgggtat
    20041 gggcgcgggc ttcgccattg atcagataca gatcgcccat ctccatatag tgaagttcgt
    20101 aaccacgacg ctgtgcttcc agcaacatag cgaaactgga atctttcttg atgttgatgt
    20161 ttgcgatggg gtccatcacg atgccgagct tgatcattat tcttctccgt tagcccaaat
    20221 cgccaaatcg tacttgtagc gcggtaatgg cggtgagcgc agttgtctct gtacgcaaaa
    20281 cgcgaggtcc caacaggata tcagtaaatt gatagcgggc agtcatggca atttcatctg
    20341 ccgataaacc gccttccggg ccaatcagca ggcggacgcg ttcaaccggt aaaggcaacg
    20401 tattgatgct gttactggcg cgcgggtgaa gattcagttt cagtccttca tcctgctctg
    20461 cacaccaggc ttccagatcc atcgctggac ggatttccgg cacccggtta cgaccacact
    20521 gctcacaggc agcaattgca atcttctgcc actgctggag cttcttgttc agacgttcac
    20581 tatccagttt aacgccgcag cgctcagaaa aaagtggcgt aatgaggctt acaccgagtt
    20641 cgatcgattt ctggatcgta aattccattt tttcaccacg cgacatcacc tgaccgaggt
    20701 gaatatgcag cggagattcg cgatcgtcga tctggccttc cagcaccttc acttccacgc
    20761 tttttttgct ggcgctggta atttcggcgt caaagacctg gttgctgccg tcaaacaatt
    20821 gcaacgcctg tcccggcccc atacgcagta cgcgcccaat atggttggcg gcatcttcgc
    20881 aaagcgcgat gtgagaatgg ctggtcagtg gttcaggatg ataaatgcgg gggatacgca
    20941 tagttaaaaa tccgcgtcgt ctccccacgc ggttgtacgc gtggggtagg ggttaacaaa
    21001 aagaatcccg ctagtgtagg ttagctcttt cgcgcctggc aagcgcgttg cacatacggg
    21061 ttatgattgc cctgtacctt cgcgatgcgt tcatcgcgct cgcactccca gtcggtaacc
    21121 ggatacatct tgtcccatgc gttgaacagc tgcgtttgct ggcgagagag tgtcaggttg
    21181 taatggtcac gcatatagaa gtaggtgcgc gcaatggcac cgcgtgcacg cgctggtggt
    21241 tcggcagctt tttctttgaa atcgaccttc atggcgcatt gaccgtactg gccttcaccg
    21301 ccattccact ggctgtacat aaagttgccg cgatcgccat tcacctcgcc gactgacggt
    21361 tgcaggttat gcatatcgct ttccatcttg cgatagaccg gatctttggc gcaattttta
    21421 cgtccaccgt cctgccagca ctggcgctgg tgaccgaatt gccaggcggg aacgacgtgt
    21481 tcccactcta cgcggctggc gcggttttca tttttgcgca cctgatagcc gcacgattgc
    21541 agatcgacaa cgcctttttt gccctgccag ttaattttac atccgcaata aaacgtaccg
    21601 ggcgcgtcag cgtggacttt taccgccgcg gctttcgcct gagaaaaact attgatacct
    21661 tcggccaacg ccgggccgga aaatgctgcg ctcagtacca ccgcagcaat agacaaataa
    21721 cggtacatca cttactccgt gttaaaacga gccagcaacg tagcgaaagt tgttttggta
    21781 tgcaatcagc cagatcagga aagttcctga tagttcagat ggttattttg cgaccagttg
    21841 ttcgccgcag tgaacacagc gatagacggc ttcgccacga actacgcgat tatgacggcg
    21901 tacggtaagc tgatgttcct gacacttaca gctgtagggg aaggtgttgc ggcgcacgga
    21961 ttgcagttcg aactgatgcg tacggcgggc tggaacgccc agcacacttt ccatcatcca
    22021 cttccactct ttgccatgtg gtgctacgcg gccaaaatgt ttccatacca gcaaatgtgc
    22081 cagttcgtgc ggtaccactt cttcaataaa ggcttcactg ttttccaaca gcaaaacggg
    22141 attaaggcga atttcatagc tttccagcca ggctgttccg gcagaggttc cgcgctgggt
    22201 gtaagagagt tttggctccg ggtagttacg ccccaatttc aggttggcct gggcgagttt
    22261 ttcccgcagg cgacgcataa cggcctgctg gatggcgata gggagacggg atgttttcat
    22321 agcggcagag gatagagcga agaaaagatg actgcaagag gtggcttcct ccacgacggg
    22381 aggaagcctg gggagattaa tcgtgagcgc cgatttcgcg cagtttacga cctttcatca
    22441 gattacgttc aatatgttcc agcgaaacgt gtttggtttc tggcaccagc cacagtgtca
    22501 gcaggataaa caggacgttc agacccgcgt aaacccagaa ggtattagcg ttgcccagcg
    22561 tgttgagcat ggtcaggaac gttgcgccaa cgatcatgtt ggcaatccag ttggtggcgg
    22621 tggagcaggt gatgccaaaa tcgcggcctt tcagcggctg aatttcggag cacagtaccc
    22681 aaatcagcgg accggcactc atggcaaaac cgacaataaa catcagcagc atggcgatgg
    22741 cgaaatactg cgccgacgga gagtggatac cgatatgcat cattgtaccg agtacgccca
    22801 taccagcagc catcaccagg aagcccagcg ttagcgttgg tttacgtccc cagcggtcaa
    22861 caaggccgat tgcgataaag gtggcaagta cgttggtcag gccgacaatc actgtccccc
    22921 acatttgctc ggtggtgttg gtataacccg ccagttcgaa gattttcggc gcgtaataca
    22981 tgatgacgtt catcccggtg aattgctgca ttacctgtaa cagtacgcca aggaacaccg
    23041 cgcggcggaa gttgctattc tctttaaaca gcgcccagcc actctgttta acctgcaaac
    23101 tttcacggat ttcatccagt tcgcgtttcg cttccgcgct ggtgtcacgc aggcgtagca
    23161 gcacgcgttc ggcatcaaca aaacggcgtt tggcggcaaa ccaacgtggg ctgtctggca
    23221 ggaagaagac accaatcagc agcaaaattg ccgggatgat aatcacaccc agcatccagc
    23281 gccatgcacc ggtgtagctg aaggcggtat cagaaagata agcaccgagg atcccgattg
    23341 tgatcattaa ctgatacatc gagatcatac tgccgcgaat tttttccggc gcaatttcag
    23401 agaggtacag tggtgcggta taagaggcca cacctaccgc cagccccagc agaacgcggg
    23461 aaagaatcag tacttcaacg tttggcgcag ccgcagagaa cagcgaaccg gcaacaaaca
    23521 aaattgcgcc gatcatcagg ctctttttgc gcccgagttt aaaggagagc cagccgctgc
    23581 ccaccgcacc gactgccgca ccgaacatca tggagcttac gacccattct tgcgtgtgcg
    23641 aagtaatctg gaattcatct gcaataaacg gcagtgcgcc agcaattaca ccgatatcca
    23701 ggccaaagag taatcccgcc agagcggcaa ggaagcagac gaaaaacgtc attgccttgt
    23761 ttgactgccc ctgtttttta gcgtcaggca tgatgccctc caatatggtt attttttatt
    23821 gtgaattaag ataggtgagt acgacgtaaa aagatgtgaa gcaaatcaca tcagtgtaat
    23881 cgcttacact aatatttatc ttagttatga tttaagtgca tgaaaataat tgagttatat
    23941 catcatagat taaacgcggt tatctgcaat taagacttta ctgaaaagaa atgtaacaac
    24001 tgtgaaaacc gaataataat atggaaatat tacaaatctt tgtctgttgt ggttggtgta
    24061 atcggtttca gttgtgatga taatttgcgg tgaaacgtga tcaggaagaa tgatgttatt
    24121 gtgcaggcgg tttttttgcc ggatgcggcg tgaacgccta tccaccgcgg tgaggtaaaa
    24181 gaaaaaggcc agcgcgaggc tggcctttga aagcaggtga agaaagatta cttcagaccg
    24241 gcagcatcgc gcagcagctg cgctttgtcg gttttttccc acgggaaatg ttcacgacca
    24301 aagtgaccgt atgctgcggt ttctttgtag atcgggtgca gcagatccag catctgaatc
    24361 agaccgtatg ggcgcaggtc gaagaactca cgtaccagca gagtcagttg ttcagaaggc
    24421 actttctcag taccgaaagt ttctaccatg atggaagtcg gttcagccac gccgattgcg
    24481 taggaaacct gaatttcaca acgatcggcc aggccagcag caacgatgtt tttcgcgaca
    24541 taacgtgctg cgtaggctgc ggaacggtcc acttttgatg gatctttacc agagaatgca
    24601 ccgccaccgt gacgcgccat gccgccgtag gtatcaacga taattttacg accagtcaga
    24661 ccgcagtcac ccatcgggcc accgataaca aaacgaccgg tcgggttgat gaagaatttg
    24721 gtggcagaag tcagccattc agcgggcaga atcggcttga tgatctcttc cattaccgct
    24781 tcttgcagcg atttctggtc gatctcttca gagtgctgag tggaaagcac gacagcatcg
    24841 ataccaacga ttttgccgtc gtcatactgg aaagtcacct ggcttttcgc gtccgggcgc
    24901 agccacggca gagtgccgtt tttacgcact tcagcctgac gctgtaccag acggtgtgca
    24961 taggtgatag gtgctggcat cagcacgtcg gtttcattag ttgcgtagcc aaacatcaga
    25021 ccctggtcac ccgcgccctg ttccagcgga tcggcacggt caacgccctg gttgatgtca
    25081 ggagactgtt tgccgatagc gctcagaact gcacaggagt tagcgtcaaa gcccatgtcg
    25141 gaatgcacat agccaatttc gcgaacggtg ttacgggtga tctcttcgat gtctacccag
    25201 gcgctggtgg tgatttcgcc gccaactaaa accatgccgg tttttacgta ggtttcgcaa
    25261 gcaacgcgtg ctttcggatc ctgttcgagg atcgcgtcta aaacggcatc agagatttgg
    25321 tcagcaattt tgtcaggatg cccttcagag acggactcgg acgtaaaaag gtgttttgcc
    25381 atatttaata tcacctaaag agaatttggt tagctcaaac tgttgtgtgg attttctgtg
    25441 gtagcggatc ctaccacgac tctgcaggtt aaaaacactg gcagtctgag tgttaatcgg
    25501 tatggatgga ttaacatctg gatggctatt ttaggtcaat tcttcacccg atttccactt
    25561 ttttttgaat cgtgtctcat tctgttaaaa acgtggctgg aaatttttcc tgacaatgcc
    25621 ggcattctgc gtatttatct tttgcaattt tctgccattg tggggtataa aacgcggcgc
    25681 gcggcttaaa taaaaagcac acgacgtttc tttcgtgttg ccacttccag ccgggttgaa
    25741 atcagagttt tggcttgtgg gttcgtctta acaggcggcc gtggaggtga tacgaaataa
    25801 tgaaccgttg tctgctgctt aacctgtctc accgttctgg tgaagattcg ttccccgcac
    25861 tctgcatctc tgctttgcat acctgccgat gttataccca tctcggcgct tctcaggatt
    25921 caagagctgg ttacagttac tgaggactga acaagggcgc tcttgtaaaa acaagagttt
    25981 tctcgtggtt tcgccgaact gtcacactta cgttcggtta tgtgcttaat aatgttatga
    26041 aaaagaaacc ggttgcgcag ttggagcgtc agcattcact gctggaaaat ccatgtgctt
    26101 atgggttgtt atcgcagttc caggctgcga tagtcgttaa ctgttttaca cttaataaaa
    26161 taatttgagg ttcgctatgt ctgacgacat gtctatgggt ttgccttcgt cagcgggcga
    26221 acacggtgta ctacgctcca tgcaggaggt tgcaatgagc tcccaggaag ccagcaagat
    26281 gctgcgtact tacaatattg cctggtgggg caataactac tatgacgtta acgagctggg
    26341 ccacatcagc gtgtgcccgg acccggacgt cccggaagct cgcgtcgatc tcgcgcagtt
    26401 agtgaaaact cgtgaagcac agggtcagcg tctgcctgca ctgttctgtt tcccacagat
    26461 cctgcagcac cgtttgcgtt ccattaacgc cgcgttcaaa cgtgcgcggg aatcctacgg
    26521 ctataacggc gattacttcc ttgtttatcc gatcaaagtt aaccagcacc gtcgcgtgat
    26581 tgagtccctg attcattcgg gcgaaccgct gggtctggaa gccggttcca aagccgagtt
    26641 gatggcagtg ctggcacatg ctggcatgac ccgtagcgtc atcgtctgca atggttataa
    26701 agaccgcgaa tatatccgcc tggcattaat tggcgagaag atggggcaca aggtctatct
    26761 ggtcattgag aagatgtcag aaatcgccat tgtgctggat gaagcagaac gtctgaatgt
    26821 cgttcctcgt ctgggcgtgc gtgcacgtct ggcttcgcag ggctccggta aatggcagtc
    26881 ctccggcggg gaaaaatcga agttcggcct ggcggcgact caggtactgc aactggttga
    26941 aaccctacgt gaagccgggc gtctcgacag cctgcaacta ctgcacttcc acctcggttc
    27001 gcagatggcg aatattcgcg atatcgcgac aggcgttcgt gaatccgcgc gtttctatgt
    27061 tgagctgcac aagctgggcg tcaatattca gtgcttcgac gtcggcggcg gtctgggcgt
    27121 ggattatgaa ggtactcgtt cgcagtccga ctgttcggtg aactacggcc tcaatgaata
    27181 tgccaacaac atcatctggg cgattggtga tgcgtgtgaa gaaaacggtc tgccgcatcc
    27241 gacggtaatc accgaatcgg gtcgtgcagt gactgcgcat cacaccgtgc tggtgtctaa
    27301 tatcatcggc gtggaacgta acgaatacac ggtgccgacc gcgcctgcag aagatgcgcc
    27361 gcgcgcgctg caaagcatgt gggaaacctg gcaggagatg cacgaaccgg gaactcgccg
    27421 ttctctgcgt gaatggttac acgacagtca gatggatctg cacgacattc atatcggcta
    27481 ctcttccggc acctttagcc tgcaagaacg tgcatgggct gagcagcttt atttgagcat
    27541 gtgccatgaa gtgcaaaagc agctggatcc gcaaaaccgt gctcatcgtc cgattatcga
    27601 cgagctgcag gaacgtatgg cggacaaaat gtacgtcaac ttctcgctgt tccagtcgat
    27661 gccggatgcg tgggggatcg accagttgtt cccggttctg ccgctggaag ggctggatca
    27721 agtgccggaa cgccgcgctg tgctgctgga tattacctgt gactctgacg gtgctatcga
    27781 ccactatatc gatggtgatg gtattgccac gacaatgcct atgccggagt acgatccaga
    27841 gaatccgccg atgctcggtt tctttatggt cggcgcatat caggagatcc tcggcaacat
    27901 gcacaacctg ttcggtgata ccgaagcggt tgacgtgttc gtcttccctg acggtagtgt
    27961 agaagtggaa ctgtctgacg aaggcgatac cgtggcggac atgctgcaat atgtacagct
    28021 cgatccgaaa acgctgttaa ctcagttccg tgatcaagtg aagaaaaccg atcttgatgc
    28081 tgaactgcaa caacagttcc ttgaagagtt cgaggcaggt ttgtacggtt atacctatct
    28141 tgaagatgaa taattaagat tattctgctg gcgggtcgat ttatttacgt aaatcggtcc
    28201 gctt
//
LOCUS       DBFIFM010000042        27360 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.042, whole genome shotgun sequence.
ACCESSION   DBFIFM010000042 DBFIFM010000000
VERSION     DBFIFM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.042"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..325
                     /locus_tag="N7C74_001601"
     CDS             <1..325
                     /locus_tag="N7C74_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2431495.1"
                     /translation="FISAFRLPAGRSADDSLEELKKMQRDNLKDQYNEETFQFHDCNA
                     KHFTCKYQDVLINFDGQQKRTISVYLEDTPRAVGIIALMEPDTADKYRQQAMEIMLSA
                     KNTVK"
     gene            complement(364..609)
                     /gene="zapB"
                     /locus_tag="N7C74_001602"
     CDS             complement(364..609)
                     /gene="zapB"
                     /locus_tag="N7C74_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HGU2431496.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            1035..1880
                     /gene="glpF"
                     /locus_tag="N7C74_001603"
     CDS             1035..1880
                     /gene="glpF"
                     /locus_tag="N7C74_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HGU2431497.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            1903..3411
                     /gene="glpK"
                     /locus_tag="N7C74_001604"
     CDS             1903..3411
                     /gene="glpK"
                     /locus_tag="N7C74_001604"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HGU2431498.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            3641..4651
                     /gene="glpX"
                     /locus_tag="N7C74_001605"
     CDS             3641..4651
                     /gene="glpX"
                     /locus_tag="N7C74_001605"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HGU2431499.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANSLA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIESGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            4748..5494
                     /gene="fpr"
                     /locus_tag="N7C74_001606"
     CDS             4748..5494
                     /gene="fpr"
                     /locus_tag="N7C74_001606"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HGU2431500.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVHPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(5499..5927)
                     /gene="uspD"
                     /locus_tag="N7C74_001607"
     CDS             complement(5499..5927)
                     /gene="uspD"
                     /locus_tag="N7C74_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HGU2431501.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(5954..6253)
                     /gene="yiiS"
                     /locus_tag="N7C74_001608"
     CDS             complement(5954..6253)
                     /gene="yiiS"
                     /locus_tag="N7C74_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HGU2431502.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(6465..6905)
                     /gene="yiiR"
                     /locus_tag="N7C74_001609"
     CDS             complement(6465..6905)
                     /gene="yiiR"
                     /locus_tag="N7C74_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGU2431503.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            7006..7605
                     /gene="yiiQ"
                     /locus_tag="N7C74_001610"
     CDS             7006..7605
                     /gene="yiiQ"
                     /locus_tag="N7C74_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HGU2431504.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            7713..8480
                     /gene="tpiA"
                     /locus_tag="N7C74_001611"
     CDS             7713..8480
                     /gene="tpiA"
                     /locus_tag="N7C74_001611"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HGU2431505.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(8535..9290)
                     /gene="cdh"
                     /locus_tag="N7C74_001612"
     CDS             complement(8535..9290)
                     /gene="cdh"
                     /locus_tag="N7C74_001612"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HGU2431506.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(9397..10386)
                     /gene="sbp"
                     /locus_tag="N7C74_001613"
     CDS             complement(9397..10386)
                     /gene="sbp"
                     /locus_tag="N7C74_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HGU2431507.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKLGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLISWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(10705..11667)
                     /gene="pfkA"
                     /locus_tag="N7C74_001614"
     CDS             complement(10705..11667)
                     /gene="pfkA"
                     /locus_tag="N7C74_001614"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HGU2431508.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(11848..12750)
                     /gene="fieF"
                     /locus_tag="N7C74_001615"
     CDS             complement(11848..12750)
                     /gene="fieF"
                     /locus_tag="N7C74_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HGU2431509.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(12899..13399)
                     /gene="cpxP"
                     /locus_tag="N7C74_001616"
     CDS             complement(12899..13399)
                     /gene="cpxP"
                     /locus_tag="N7C74_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HGU2431510.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            13549..14247
                     /gene="cpxR"
                     /locus_tag="N7C74_001617"
     CDS             13549..14247
                     /gene="cpxR"
                     /locus_tag="N7C74_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HGU2431511.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            14244..15617
                     /gene="cpxA"
                     /locus_tag="N7C74_001618"
     CDS             14244..15617
                     /gene="cpxA"
                     /locus_tag="N7C74_001618"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HGU2431512.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(15723..16397)
                     /gene="yiiM"
                     /locus_tag="N7C74_001619"
     CDS             complement(15723..16397)
                     /gene="yiiM"
                     /locus_tag="N7C74_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HGU2431513.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(16546..17529)
                     /gene="kdgT"
                     /locus_tag="N7C74_001620"
     CDS             complement(16546..17529)
                     /gene="kdgT"
                     /locus_tag="N7C74_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HGU2431514.1"
                     /translation="MQIKRLIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(17789..18409)
                     /gene="sodA"
                     /locus_tag="N7C74_001621"
     CDS             complement(17789..18409)
                     /gene="sodA"
                     /locus_tag="N7C74_001621"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HGU2431515.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            18694..19728
                     /gene="rhaT"
                     /locus_tag="N7C74_001622"
                     /pseudo
     CDS             18694..19728
                     /gene="rhaT"
                     /locus_tag="N7C74_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(19725..20573)
                     /gene="rhaR"
                     /locus_tag="N7C74_001623"
     CDS             complement(19725..20573)
                     /gene="rhaR"
                     /locus_tag="N7C74_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HGU2431516.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(20647..21483)
                     /gene="rhaS"
                     /locus_tag="N7C74_001624"
     CDS             complement(20647..21483)
                     /gene="rhaS"
                     /locus_tag="N7C74_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HGU2431517.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYHCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            21771..23240
                     /gene="rhaB"
                     /locus_tag="N7C74_001625"
     CDS             21771..23240
                     /gene="rhaB"
                     /locus_tag="N7C74_001625"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HGU2431518.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALISATQALPACRFIINP
                     NDDRFINPETMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVARIHSTRQTKELCA"
     gene            23237..24496
                     /gene="rhaA"
                     /locus_tag="N7C74_001626"
     CDS             23237..24496
                     /gene="rhaA"
                     /locus_tag="N7C74_001626"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HGU2431519.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWIIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKETLSRRG"
     gene            24671..25495
                     /gene="rhaD"
                     /locus_tag="N7C74_001627"
     CDS             24671..25495
                     /gene="rhaD"
                     /locus_tag="N7C74_001627"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HGU2431520.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAEVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            25505..25819
                     /gene="rhaM"
                     /locus_tag="N7C74_001628"
     CDS             25505..25819
                     /gene="rhaM"
                     /locus_tag="N7C74_001628"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HGU2431521.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTEICQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(25860..27254)
                     /locus_tag="N7C74_001629"
     CDS             complement(25860..27254)
                     /locus_tag="N7C74_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HGU2431522.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLMLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATAESAKKNATSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKMDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPDNGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
BASE COUNT         6774 a         7067 c         6869 g         6650 t
ORIGIN      
        1 ctttatttct gctttccgcc tgcccgcagg acgtagcgcc gatgactcgc tggaagagtt
       61 aaagaagatg cagcgagaca atctcaagga tcaatacaat gaagaaacct tccagtttca
      121 tgattgtaat gccaaacatt tcacctgtaa gtatcaggat gtgttgataa attttgatgg
      181 tcagcagaaa agaaccatca gtgtttatct tgaagacacc cccagggctg tggggatcat
      241 tgcattaatg gagccagata cggcagataa atatcgccaa caggcgatgg agattatgtt
      301 gtctgctaaa aatacagtga agtaaaaaat aaggcgcctt gcggcgcctc tgcgtggaag
      361 agattaaacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc agccgttctg
      421 ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct gggcattttg
      481 aacttcctgc gacaatgagt tgtttttttc tttcagctct tcgatttcca tctgcaacag
      541 agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca cttctaatga
      601 cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct cgtcccgatt
      661 accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt atgaagccgc
      721 gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt tcatccttct
      781 cctggtgaca taatccacac caatcgaaaa tgttaataaa tttgttgcgc gaatgttcta
      841 acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct cattttatga
      901 cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg ataagtttac
      961 agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa acattaactc
     1021 ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt gctgaattcc
     1081 tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta aaagtcgctg
     1141 gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg gcaatggcca
     1201 tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc attgcattgt
     1261 ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca caagttgccg
     1321 gcgctttctg tgctgcggct ttagtttacg ggctttacta caatttattt ttcgacttcg
     1381 agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggca ggcactttct
     1441 ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag atggtgatta
     1501 ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt gtaccacgcg
     1561 gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca tctatgggcc
     1621 cattgacggg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc tttgcctggc
     1681 tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac ttcctggtgc
     1741 cgctttttgg ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc aaactgattg
     1801 gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggagaccaca actccttcag
     1861 aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga aaaaaaatat
     1921 atcgttgcgc tcgaccaggg caccaccagc tcccgcgcgg tcgtaatgga tcacgatgcc
     1981 aatatcatta gcgtgtcgca gcgcgaattt gagcaaatct acccaaaacc aggctgggta
     2041 gaacacgacc caatggaaat ctgggccacc caaagctcca cgctggtaga agtgctggcg
     2101 aaagccgata tcagttccga tcaaattgca gctatcggta ttacgaacca gcgtgaaacc
     2161 actattgtct gggaaaaaga aaccggcaag cctatctata acgccattgt ctggcagtgc
     2221 cgtcgtaccg cagaaatctg cgagcattta aaacgtgacg gtttagaaga ttatatccgc
     2281 agcaataccg gtctggtgat tgacccgtac ttttctggca ccaaagtgaa gtggatcctc
     2341 gaccatgtgg aaggctctcg cgagcgtgcg cgtcgtggtg aattgctgtt tggtacggtt
     2401 gatacgtggc ttatctggaa aatgactcag ggccgtgtcc atgtgaccga ttacaccaac
     2461 gcctctcgta ccatgttgtt caacatccat accctggact gggacgacaa aatgctggaa
     2521 gtgctggata ttccgcgcga gatgctgcca gaagtgcgtc gttcttccga agtgtacggt
     2581 cagactaaca ttggcggcaa aggcggcacg cgtattccaa tctccgggat cgccggtgac
     2641 cagcaggccg cgctgtttgg tcagttgtgc gtgaaagaag ggatggcgaa gaacacctat
     2701 ggcactggct gctttatgct gatgaacact ggcgagaaag cggtgaaatc agaaaacggc
     2761 ctactgacca ccatcgcctg cggcccgact ggcgaagtga actatgcgtt ggaaggtgcg
     2821 gtgtttatgg caggcgcatc cattcagtgg ctgcgcgatg agatgaagtt gattaacgac
     2881 gcctacgatt ccgaatattt cgccaccaaa gtgcaaaaca ccaatggtgt gtatgtggtt
     2941 ccggcattta ccgggctggg tgcgccgtac tgggacccgt atgcgcgcgg ggcgattttc
     3001 ggtctgactc gtggggtgaa cgctaaccac attatacgcg cgacgctgga gtctattgct
     3061 tatcagacgc gtgacgtgct ggaagcgatg caggccgact ctggtatccg tctgcacgcc
     3121 ctgcgcgtgg atggtggcgc agtagcaaac aatttcctga tgcagttcca gtctgatatt
     3181 ctcggcaccc gcgttgagcg cccggaagtt cgcgaagtca ccgcattggg tgcggcctat
     3241 ctcgcaggcc tggcggttgg cttctggcag aacctcgacg agctgcaaga gaaagcggtg
     3301 attgagcgcg agttccgtcc aggcatcgaa accactgagc gtaattaccg ttacgcaggc
     3361 tggaaaaaag cggtcaaacg cgcgatggcg tgggaagaac acgacgagta atgtcaggca
     3421 cctgaatcga ttgcctgatg ctgatcgcct gatgcgacgc ttacgcgtct tatcaggcct
     3481 acgagccaat gccattttgt gtaggcggga taaggcgttc acgccgcatc cggcatcacg
     3541 cagactctga aaccgtgcgg gggcaacccc gcacacatca ataatcccac ccttcccctg
     3601 tgctacactt cgcgccattc cttactgctt agagtttgct atgagacgag aacttgccat
     3661 cgaattttcc cgcgtcaccg aatctgcggc gctggctggc tacaaatggt taggacgcgg
     3721 cgataaaaac accgcggacg gcgcggcggt aaacgccatg cgtattatgc tcaaccaggt
     3781 caacattgac ggcaccatcg tcattggtga aggtgaaatc gacgaagcac cgatgctcta
     3841 cattggtgaa aaagtcggta ctggtcgcgg cgacgcggta gatattgctg ttgatccgat
     3901 tgaaggcacg cgcatgacgg cgatgggtca ggctaactcg ctggcggtgc tggcagtagg
     3961 cgataaaggc tgtttcctca acgcgccaga tatgtatatg gagaagctga tcgtcggacc
     4021 gggagccaaa ggcaccattg atctgaacct gccgctggcg gataacctgc gcaatgtcgc
     4081 ggcggcgctc ggcaaaccgt tgagcgaact gacggtaacg attctggcta aaccacgcca
     4141 cgatgccgtt atcgctgaaa tgcagcaact cggcgtacgc gtatttgcta ttccggatgg
     4201 cgacgttgcg gcctcaattc tcacctgtat gccagacagc gaagttgacg tgctgtacgg
     4261 aattggtggc gcgccggaag gcgtggtttc tgcggcggtg atccgcgcat tagatggcga
     4321 catgaacggt cgtctgctgg cgcgtcatga cgtcaaaggc gacaacgaag agaatcgtcg
     4381 cattggcgag caggagctgg cacgctgcaa agcgatgggc atcgaatccg gtaaagtatt
     4441 gcgtctgggc gatatggcgc gcagcgataa cgtcatcttc tctgccaccg gtattaccaa
     4501 aggcgatctg ctggaaggca ttagccgcaa aggcaatatc gcgactaccg aaacgctgct
     4561 gatccgcggc aaatcacgca ccattcgccg cattcagtct atccactatc tggatcgcaa
     4621 agacccggaa atgcaggtgc acatcctctg attgatttga tcgattgagc cttccagtcc
     4681 ttcgggactg gaattttttt gttcggagaa cgaagataag gcaattcaat caaaacagga
     4741 gaaaaacatg gctgattggg taacaggcaa agtcactaaa gtgcagaact ggaccgacgc
     4801 cctgtttagt ctcaccgttc acgcccctgt gcatccgttt accgccgggc aatttaccaa
     4861 gcttggcctt gaaatcgacg gcgaacgcgt ccaacgcgcc tactcctatg ttaactcgcc
     4921 cgataatccc gatctggagt tttacctggt caccgtcccc gatggcaaat taagcccacg
     4981 attggcggca ctgaaaccgg gcgatgaagt gcaggtggtt agcgaagcag ctggcttctt
     5041 tgttctggat gaagtaccag attgcgaaac gctatggatg ctggcaaccg gtacagcgat
     5101 tggcccttat ttatcgattc tgcaactagg taaagattta gatcgcttca aaaatctggt
     5161 cctggttcac gccgcacgtt atgccgccga cttaagctat ttgccactga tgcaagaact
     5221 ggaaaaacgc tacgaaggca aactgcgcat tcagacggtg gtcagtcggg aaacggcagc
     5281 ggggtcgctc accggacgga taccggcatt aattgaaagt ggtgaactgg aaagcgcgat
     5341 tggcctgccg atgaataaag aaaccagcca tgtgatgctg tgcggcaatc cacagatggt
     5401 gcgcgatacg caacagttgc tgaaagagac ccggcagatg acgaaacatt tacgtcgccg
     5461 accgggtcac atgacagcgg agcattactg gtaagcggtt acttatcgat aaacggcacg
     5521 atgagcaaat ccgcactcaa cttattgatc atcccgcgat atgccggcat caaacggttg
     5581 ataaatgagt ggtgatgacc acagacaagg aggtcgcact gctctttttg cataatttcc
     5641 agcagtgttt ccggcatttc tccgcgttca atacgcagtt ttgtcttcgg ccattgaata
     5701 tttttcgtca gtttatacag cttgttatcc gacttattct tcaacaattg aagaatatct
     5761 tctgttgcag ggaagtagat acccggatac aactcgctta agccatcatc aatatgaatt
     5821 aacgtcaggt gagcgtcatt atgtctggcg agctccaagg ctttattcac cagtaaggca
     5881 tcttcttcat tcccggaaat tgccacgcca atgtgtttat aagccatgtt taactccttc
     5941 taaagcaact ccatcaagct aacgaacgca gtgataactc aaaaataatc atctcggcct
     6001 ggcatgagaa ggtgaaggcc gcatcaagct caacttgccc ctccagatcc ttaatcttat
     6061 aggccacgct ggccccttca tctgcagagg atgcagcggc ggcaagctct ttcagtttgg
     6121 tcataaagga ctctgcttct tcgcgggtgg caaaaaagcg cgaaaactta ctggtacagt
     6181 tgtcgttatc aatcaccgta ccgatatcaa tcgcacatcc tttagtactg catttatcta
     6241 cgacatcttt catagggcac ctctgtatat tcgcctctct gtcgattacg acttaagaat
     6301 aatgcgccgt taagggaaga acaaacaagt aatgatctta tggattacca ttttttttga
     6361 tattgcgtaa ggtgaagagt tcagaaggat tttccgggta ttgtcatttg catcgtggaa
     6421 ataacaatcc cggaaaaaaa tagcagtaag agatatatta aaggttaatt acttgattta
     6481 ttgtcggctt tatacttcac atcctgagta tctttaccat atttattttc gccttgtgtg
     6541 ccaacaaacg caccaagatc gataagcatc atcaccagaa tcaacgtcgg gacaaaacgc
     6601 cctaccgccc attgccagac acccggtaaa atcgcccagt tacccgccag tagcatccac
     6661 gccacaatca tcagaaatgc ccatgcgccg gaacgcccgc gatcatgcaa gcgcttaacg
     6721 gttactgccg ctgttggcca gagcaagcac acaaggcaaa acgccgcggt ctgaatatcg
     6781 agtaaatttt tacccgccag tgagaaaagc accagcatgc ctgcgaacca caggcctatc
     6841 caaatccaga aatcacggcg tccaatacgc cctttaaatg agaataacca ttgctgtatg
     6901 gtcatgtaag ttccttgatg gttgtctttt ccaggattct acccttttga caagggcgac
     6961 agatatcgtt ttaatcggag ccagtcataa caaaaggtac tgtcaatgaa gccagggtgt
     7021 acgctgtttt ttctcttatg ttctgcatta accgttacaa caacggcgca tgcacaaaca
     7081 ccagatacgg caacgaccgc gccttatctg ctggctggag cccctacttt cgatctctcc
     7141 atcagccagt ttcgagaaga ctttaacagc cagaatccca gcctgccact gaacgaattt
     7201 cgtgccatcg acagcagtcc cgacaaagcc aatctcactc gtgctgccag taaaattaac
     7261 gagaacttgt atgcttctac agcgctggag cgcggtacct taaaaatcaa aagcattcaa
     7321 atgacatggc tacccatcca ggggccagag caaaaagccg cgaaagcgaa agctcaggaa
     7381 tacatggcag cggtgatccg cacactcacc ccattaatga ccaaaacaca aagccagaaa
     7441 aaactgcagt cgctactaac ggcggggaaa aacaaacgtt attacaccga gacagaaggt
     7501 gcactgcgct atgttgtcgc ggacaacggc gaaaaggggc tgaccttcgc tgttgaaccg
     7561 attaagctgg cgctatctga atcgcttgaa ggtttgaata aatgacaaaa agcaaagcct
     7621 ttgtgccgat gaatctctat actgtttcac agacctgctg ccctgcgggg cggccatctt
     7681 cctttattcg cttataagcg tggagaatta aaatgcgaca tcctttagtg atgggtaact
     7741 ggaaactgaa cggcagccgc cacatggttc acgagctggt ttctaacctg cgtaaagagc
     7801 tggcaggtgt tgctggctgt gcggttgcaa tcgcaccacc ggaaatgtac atcgatatgg
     7861 cgaagcgtga agctgaaggc agccacatca tgctgggtgc gcaaaacgtg gacctgaacc
     7921 tgtccggcgc attcaccggt gaaacctctg ctgctatgct gaaagacatc ggcgcacagt
     7981 acatcatcat cggtcactct gaacgtcgta cttaccacaa agaatctgac gaactgatcg
     8041 cgaaaaaatt cgcggtgctg aaagagcagg gcctgactcc ggttctgtgc atcggtgaaa
     8101 ccgaagctga aaacgaagcg ggcaaaactg aagaagtttg cgcacgtcag atcgacgcgg
     8161 tactgaaaac tcagggtgct gcggcattcg aaggtgcggt tatcgcttac gaacctgtat
     8221 gggcaatcgg tactggcaaa tctgcaactc cggctcaggc acaggctgtt cacaaattca
     8281 ttcgtgacca catcgctaaa gttgacgcta acatcgctga acaagtgatc attcagtacg
     8341 gcggctctgt aaacgcgtct aacgctgcag aactgtttgc tcagccagac atcgacggcg
     8401 cgctggttgg cggtgcgtct ctgaaagctg acgctttcgc agtaatcgtt aaagctgcag
     8461 aagcggctaa acaggcttaa gtctgacagg tgccggattt catatccggc acttactttc
     8521 cttaactctt cgccttaacg caaaatctca cactgatgat cctgaatttc ctcggctgaa
     8581 gcacggttaa gcgtcagtag atttcgttgt gtcgccagca atacaaatga gttatcactc
     8641 tgccgtacca tcgccagccc gtagcttccc atatgttccc gcgcctcagg tacttcttct
     8701 gccagcatca taaatgggct gcgttgtacc agttcgcttt ccgttacccg acgcgccagg
     8761 tattcatgcc cgcgcaaacc acctggcagt ggcaaccagc ggctgctgat gttcgccaga
     8821 ttgttatcca gctgttcgcg cacatcagga cgaatacaag agatatgaat atgaaaatgg
     8881 ttttgcgtac gcccggtgcg ggagttgatc gccaaagaaa ccgcgcgatc gggaaccggc
     8941 tggccgtatt ttttgctcat aaaatcacgc gcctgccagg ccaaccaaaa gaagttcggc
     9001 gttgaaggat cggtcaacaa aggactttca gtaccgttaa tacgatacgt tggcatcaac
     9061 agatattgca gtgggccatt aagatctttt aaaaccacgt atccggcatt gggtttgact
     9121 tccgcacatg gcgaaggatt ttgattttgc tgctgattgg gcaaacattc ctcaaggaca
     9181 atcttacgta atgtatccga ctcttcaccg gttaatttcc agtaaccaat accggcagcc
     9241 acaacggcga taactatcat caccaaaaaa agaagacccg cttttttcat ctttttttcc
     9301 ctgtacctca aagagatgca agggtaacgc aaaatcgtga caaataaaaa acccggtcgc
     9361 gagaaaccac cgggtttgat aattatcctg gggaaatcag cgtttgctga tctgatcgaa
     9421 cgtaccgccg ttagcaaaat gctctttttg cgctttcgtc cagccgccga actcttcatc
     9481 aatggtgaat aacttcagct ttggaaacgc attttcgtac tttttcgcca cctcagcgtc
     9541 gcgcggacgg tagtagtttt tcgcggcaat ttcctgacct tctggcgagt agagatattt
     9601 caggtaggct tccgccacct ctttagtacc ttttttctcg accactttat cgaccaccga
     9661 cacggttggc tctgcgagga tagactcact cggcgtgacg atttcgaatt tatctttccc
     9721 cagttcattc gctgccagca gagcttcgtt ttcccaggaa atcagtacat cgccaattcc
     9781 gcgctcgaca aaagtgttag tggagccgcg cgcgccagaa tccagaactt cgacgttttt
     9841 atacagtgcc cgaacaaaat cctgtgcttt tgcctgatcg ttgttgttgt gatgcagcgc
     9901 gtagccccag gctgccaggt agttccagcg cgcgccacca gagcttttcg gattaggcgt
     9961 gatcaccgaa acacccagtt taatcagatc gttccagtca tggatctgct tcggatttcc
    10021 cttacgtacc aggaaaacaa tggtggaagt gtacggtgcg gagttatccg gcagacgttt
    10081 gatccactct ttatcaatcc gcccgcgttc cgcaatcgcg tccacgtcat aggccagagc
    10141 cagcgtgaca acatcagctt caataccgtt gattaccgac gttgcttgtt tgcctgaacc
    10201 accgtgtgac tgacgaatca ccacgttatc gccagtttgc tgtttccagt gggcgctgaa
    10261 tgccttgttg tactgttcgt acaattcgcg cgttggatca tatgaaacgt taagaagctg
    10321 aatatccttt gccataacgc tggttgccgc cagcaaaaat gttaacccta cgccccactt
    10381 gttcatcgcc cgactctctt atgttgtgtt gtgatgagca aagcgtgcca gaaggttaac
    10441 caaacattaa agaataaaaa aagattggct ataacttgcg ggtatatgtt gggggttaaa
    10501 aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttatcgat gccggatgcg
    10561 gcgcgaacgc cttatccggc ctacaaaagt ttgtaaattc aataaattgt agaattcacg
    10621 taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt tcaggctaaa
    10681 ggaatctgcc tttttccgaa atcattagta cagttttttc gcgcagtcca gccagtcgcc
    10741 tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa ccagctgttc
    10801 gttctgtata cctacgcaac gaccgccgta acctgccagc agcagatcga tagcgtaagc
    10861 gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc gctggatgtg
    10921 gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat gcgccagttc
    10981 gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac ctttcgcgat
    11041 acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg gaaccacaac
    11101 gaattcacag ccaccggcaa tggccgcagc cagcgtcaga tcgccacaat aacggcccat
    11161 cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac ggtcgatcgc
    11221 ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc ctttgatgtc
    11281 gttgtcgata gtgcccggca ggccgatgca cgggaagccc atttcggtca gacgcattgc
    11341 acccatgtag gaaccgtcac cgccgataac caccagcgca tcgatcccac gttttttcag
    11401 gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac gcgcagaacc
    11461 gaggaacgta ccgccacggt tgatcatgtc agaaacgctg taacggtcta gctgtaccat
    11521 acggtcttca tacagaccca gatagccgtc ataaatgccc attacttcca gaccttctgt
    11581 cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat caccgccgct
    11641 tgtcaacaca ccgattttct taatcatgac tacctctgaa ctttggaatg caaaataaaa
    11701 tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca aatagtataa
    11761 caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt atacacaaaa
    11821 tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac cctccctagg
    11881 tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac gccgtaaaat
    11941 agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag agtcttccat
    12001 ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa gatcgtgagc
    12061 gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt cctcatcagg
    12121 taatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg cgctatataa
    12181 aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc cgtaccagga
    12241 caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt aatgtagcat
    12301 atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caacgctgaa acgagacaag
    12361 gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg gatctgtcat
    12421 cggtgttgga gatatcagat gttgaatgcc tgtcaaaaac aggaatagtg ccgaaccgga
    12481 gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac caaacgagtg
    12541 attatcgtcg gcaggttgca gggaatatcg caccaccaat aaattcgtca acgacgcgcc
    12601 gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg tataccacca
    12661 tgcaaaaatt ttaatcagca atagcagtga agccatcgcc gtcgcagcaa tcgccgcccg
    12721 actgaccagc cgtccataag attgattcat aaatactccc gctatcaact gacgctagta
    12781 taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga taaaaaaaac
    12841 ccccacatca tgtgggggaa gacagggatg gtgtctatgg caaggaaaac agggtttact
    12901 actgggaacg tgagttgcta ctactcaata gcttcaacga tgaacttttt tgccattgcg
    12961 tcacgtcacg caactgctcc attcgttgtt gatgtttctc gtttaaaacc gcttgctgct
    13021 ccggcgttaa caggcgatac atttggttgc ggacttttgc catctcaacc tgacgagcaa
    13081 tttgctcatt cgccattttt tctgcctgtg cgcgcacagc gttttcatca aaattttctg
    13141 cggtgacaag acgatgcatt gtctccagtt cgctaacatt aacaggaggc tgttcgtgcc
    13201 gggcctgttg cataagatct cgcatctgct gacgctgatg ttcggttaaa cttatgccgt
    13261 cgaacatatg gctctgcgta ctgcgctgcg taagttcttc acccggatgc cagttatcgc
    13321 ctgaaccgac ttcagcagcg tggcttaatg aactgactgc cagcgttgag gccatgacgg
    13381 cagcggtaac tatgcgcatc atttgctccc aaaatctttc tgtcgcgatt caacgataga
    13441 gagtttacga ttcaggctgc aaacatgcgt cagggggtgt aaaacaacgt aaagtcatgg
    13501 attagcgacg tctgatgacg taatttctgc ctcggaggta tttaaacaat gaataaaatc
    13561 ctgttagttg atgatgaccg agagctgact tccctattaa aggagctgct cgagatggaa
    13621 ggcttcaacg tgattgttgc ccacgatggg gaacaggcgc ttgatcttct ggacgacagc
    13681 attgatttac ttttgcttga cgtaatgatg ccgaagaaaa atggtatcga cacattaaaa
    13741 gcacttcgcc agacacacca gacgcctgtc attatgttga cggcgcgcgg cagtgaactt
    13801 gatcgcgttc tcggccttga gctgggcgca gatgactatc tcccgaaacc gtttaatgat
    13861 cgtgagctgg tggcacgtat tcgcgcgatc ctgcgccgtt cgcactggag cgagcaacag
    13921 caaaacaacg acaacggttc accgacactg gaagttgatg ccttagtact gaatccaggc
    13981 cgtcaggaag ccagcttcga cgggcaaacg ctggagttaa ccggtactga gtttaccctg
    14041 ctctatttgc tggcacagca tctgggtcag gtggtttccc gtgaacattt aagccaggaa
    14101 gtgctgggca aacgcctgac gcctttcgac cgcgctatcg atatgcacat ttccaacctg
    14161 cgtcgtaaac tgccggatcg taaagatggt cacccgtggt ttaaaacctt gcgtggtcgc
    14221 ggctacctga tggtttctgc ttcatgatag gcagcttaac cgcgcgcatc ttcgccatct
    14281 tctggctgac gctggcgctg gtgttgatgt tggttttgat gttacccaag ctcgattcac
    14341 gccagatgac cgagcttctg gatagcgaac agcgtcaggg tctgatgatt gagcagcatg
    14401 ttgaagcgga gctggcgaac gatccgccca acgatttaat gtggtggcgg cgtctgttcc
    14461 gggcgattga taagtgggca ccgccaggac agcgtttgtt attggtgacc accgaaggcc
    14521 gcgtgatcgg cgctgaacgc agcgaaatgc agatcattcg taactttatt ggtcaggccg
    14581 ataacgccga tcatccgcag aagaaaaagt atggccgcgt ggaactggtc ggtccgttct
    14641 ccgtgcgtga tggcgaagat aattaccaac tttatctgat tcgtccggcc agcagttctc
    14701 aatccgattt cattaactta ctgtttgacc gcccgctatt actgctgatt gtcaccatgt
    14761 tggtcagtac gccgctgctg ttgtggttgg cctggagtct ggcaaaaccg gcgcgtaagc
    14821 tgaaaaacgc tgccgatgaa gttgcccagg gaaacttacg ccagcacccg gaactggaag
    14881 cggggccaca ggaattcctt gccgcaggtg ccagttttaa ccagatggtc accgcgctgg
    14941 agcgcatgat gacctctcag cagcgtctgc tttctgatat ctctcacgag ctgcgcaccc
    15001 cgctgacgcg tctgcaactg ggtacggcgt tactgcgccg tcgtagtggt gaaagcaagg
    15061 aactggagcg tattgaaacc gaagcgcaac gtctggacag catgattaac gacctgttgg
    15121 tgatgtcacg taatcagcaa aaaaacgcgc tggttagcga gaccatcaaa gccaatcagt
    15181 tgtggagtga agtgctggat aacgcggcgt tcgaagccga gcaaatgggc aagtcgttga
    15241 cagttaactt cccgcctggg ccgtggccgc tgtacggcaa cccgaacgcc ctggagagtg
    15301 cgctggaaaa cattgttcgt aatgccctgc gttattccca tacgaagatt gaagtgggct
    15361 ttgcggtaga taaagacggt atcaccatta cggtggacga cgatggtcct ggcgttagcc
    15421 cggaagatcg cgaacagatt ttccgtccgt tctatcggac cgatgaagcg cgcgatcgtg
    15481 aatctggcgg tacaggtttg ggactggcga ttgttgaaac cgccattcag cagcatcgtg
    15541 gctgggtgaa agcagaagac agcccgctgg gcggtttacg gctggtgatt tggttgccgc
    15601 tgtataagcg gagttaaact ccgcatttgt aggcaggata aggcgtttac gccgcatccg
    15661 gcatttgagc aggatgcctg atgcgacgct gatagcgtct tatcaggcct acactccggg
    15721 ggttattttc cccacaaccg gcgggaaaaa tcttcgatct tgccgctcag tcggcgcttt
    15781 tgcatcgttc gcgtccagct tttcgataac ccggcagcgg agagtaaacg gtgatactga
    15841 tcgtcatcaa acggcatatg ccatgcgatg gcagcagcct cctgcacggt cacatcgctg
    15901 acacgggaaa ccaactccag cggcgcgtcc gcagacactt tccctggcgc tatcacgcta
    15961 tacaaccatc ccactttacc cgtgttttgc atcaactgcg ctatatcgct gatatcaaaa
    16021 tggtaattga gtttatagca aggcgagcgc ggctggctga cctgaattaa tgcctctccc
    16081 cagcggaaaa tatcgccaat ataaacatta ctttccgtga gaccgtcggt tgagagattt
    16141 tcgccaaacg caggcgcaac aaacaactcc gcctgttccg gaaattcccg cgcccagtag
    16201 agataatgct cacgaggata atgacacagc gctctgtctg gtccgccgtg aacctttttc
    16261 tccgcctgct cgtcaccttc cagccccagc tccgttagca tcaactcacc atcaacctgg
    16321 attttggcaa ttgcgctggg tttgccttcg ggataagcct gaatcttgcc tgtgtataca
    16381 tcaaccggat atcgcatctg ttccctctcc acgagaaatt tccccattag accatattta
    16441 ttttatgtca ggcgtgctgt tttcgcggta agtatccagg caggaactca atagcaaacc
    16501 ggacgaacgt tagttcatcc ggctgtgggg attaaggaag caggtttatt tcaccgtacc
    16561 taaaatttcg attttcgccg ctctggcttt gactttacgt gaccagatag aggtaagaat
    16621 tggcaccaga atcgaagtca caatgactgc cgtcgctacc agcgaagttg ctgccggagc
    16681 catcggttta aacgcaggca ccatttctgc aatcagcaca ggtgtcgcta ccgcggcccc
    16741 tgcggaactg gaagcggcaa ttccggcagt gccatcgcca ccgccaatca atttatcggc
    16801 gataatcaac ggaataccgg tcacgataat tactgccaca cccaacagga tccccagcaa
    16861 acctgtctgg gcaattacag tcaaatcaat ggtattgcct agtgcgaagg caaagaatgg
    16921 aatcagcgtc tgcaccgctt tgctgaaaaa ttctcgtaat tcagggtcaa ggttcccaag
    16981 ggcaaagccc accaggaacg gtaatactgc gccgacgaaa acatgcggtt caaacgaggc
    17041 aatcccggca gtgcccagaa taatcatcgt catgagcggc ccggactcca acgacatcaa
    17101 cacaaatgcc ccagcttctt cttttgtgcc gtactgctgc atgatggaag cgtaaagtcc
    17161 gccgttggtc atatccatcg ccgccaccag cgccagcgtt gaaagtccgg caaagaatcc
    17221 aacttcaaca ccatgttccg gaataatgcg cgaggcaatt gccgcaacca cccacgcgac
    17281 ggcaatttta gttaccacca gcgtaccgga tttacgcagt accgttcccg tcgcgcttaa
    17341 ttttattgac gcccccatgc aaaaaaacca caccgccaga atgggcaccg taccggtaat
    17401 catcccgttg gtgaatgatc caaaatattt ccccgccccc ggcgagaagg tgtggcacag
    17461 tgcgccaagg aatagcggga cgagcatcat cccccccggg attttctcaa tcaagcgttt
    17521 tatctgcatt tccatcacct gtcggcatat ttttaagggc tgatgcgtaa ttcctcaatg
    17581 gagcatcagt gttgatggaa acgaagatag acattcattt aatcaataaa agtgatcaaa
    17641 accacatcaa ctgaaacgct gttttatttt tatcggatca ttgtttttat tttgaaattg
    17701 atcacaaaaa aaccaccgcc attggcgatg gttcattttt taagctgata tgcggcgtaa
    17761 acgcctcact gcagcagacg gcaaatgatt attttttcgc cgcgaaacgt gccgctgctt
    17821 cgtcccagtt caccacgttc cagaactctt tgatgtagtc cgggcggcgg ttctggaatt
    17881 tcaggtagta agcatgctcc cacacatcca ggcccagaat cgggaagccg gatgcgccag
    17941 aaatagcttc acccatcagc ggggagtcct ggttagcagt agaaaccacc gccagtttat
    18001 cgcctttcag caccagccat gcccagccgg aaccaaagcg ggaagctgcc gctttctcaa
    18061 attctgcttt gaagttatct acggaaccga aatcacgttc gatagccgct ttcaggtcac
    18121 cctgcagggt ggtgcctttt ttcagacctt tccagaacag gctgtggtta gcgtgaccgc
    18181 cagcgttgtt gcgcagtacg gttttcttgt ctgctggcag ctggtccagt ttggtgatca
    18241 actcttcaac cggcaggttg gcaaattctg gcaggctttc caacgccgcg ttggcgttgt
    18301 tgacgtaggt ctgatggtgt ttggtgtggt ggatttccat ggtctgctta tcgaagtgcg
    18361 gttccagggc atcgtaagca tacggcaggg atggcagggt atagctcata ttcatctcca
    18421 gtattgtcgg gcggccgatt gttaatgccg cgtaagcagt tggttcatta tagttaatta
    18481 aatgatattg aaaatgatta tcaatgccgt acttttcgta agggtatggt tttgcaggaa
    18541 aatgcccgag atgtgaagca aatcacccac ttaatgccgt gattgccagt aaatcgacaa
    18601 cggcggcaac aggcgaaagg ttaatcgaca gcacgatttt tacactcatc tcgtcggaga
    18661 tgtgacgcga cgaaaaatga tgaggataag aagatgagta acgcgattac gatggggata
    18721 ttttggcatt tgatcggcgc ggccagtgca gcctgttttt acgctccgtt caaaaaagta
    18781 aaaaaatggt catgggaaac catgtggtca gtcggtggga ttgtttcgtg gattattctg
    18841 ccgtaggcca tcagcgccct gttactaccg aatttctggg cgtattacag ctcgtttagt
    18901 ctctctacgc tgctgcctgt ttttctgttc ggcgctatgt gggggatcgg taatatcaac
    18961 tacggcctga ccatgcgtta tctcggcatg tcgatgggaa ttggcatcgc cattggcatt
    19021 acgttgattg tcggtacgct gatgacgcca attatcaacg gcaatttcga tgtgttgatt
    19081 aacaccgaag gcggacgcat gacgttgctc ggcgttctgg tggcgctgat tggcgtaggg
    19141 attgtaactc gagccgggca gttgaaagag cgcaagatgg gcattaaagc cgaagagttc
    19201 aatctgaaaa aaggactggt gctggcggtg atgtgcggca ttttctctgc cgggatgtcc
    19261 tttgcgatga acgccgcaaa accgatgcat gaagccgctg ccgcactagg cgtcgatcca
    19321 ctgtatgtcg ctctgccaag ctatgttgtc atcatgggcg gcggcgcgat catcaacctc
    19381 ggtttctgtt ttattcgtct ggcaaaagtg aaggatttat cgctaaaagc cgacttctcg
    19441 ctggcaaaac cgctgatcat tcacaatgtg ttactctcag cactgggcgg gttgatgtgg
    19501 tatctgcaat tctttttcta tgcctggggc cacgcccgca ttccggcgca gtatgactac
    19561 atcagttgga tgctgcatat gagtttctat gtattgtgcg gcggtatcgt cgggctggtg
    19621 ctgaaagagt ggaacaatgc aggacgccgt ccggtaacgg tgttgagcct cggttgtgtg
    19681 gtgattattg ttgccgccaa catcgttggc atgggcatgg cgaattaatc tttctgcgaa
    19741 ttgagatgac gccactggct gggcgtcatc ccggtttccc gggtaaacac caccgaaaaa
    19801 tagttactat cttcaaagcc acattcggtc gaaatatcac tgattaacag gcggctatgc
    19861 tggagaagat attgcgcatg acacactctg acctgccgca gatattgatt gatggtcatt
    19921 ccagtctgct ggcgaaattg ctgacgcaaa acgcgctcac tgcacgatgc ctcatcacaa
    19981 aatttatcca gcgcaaaggg acttttcagg ctagccgcca accgggtaat cagcttatcc
    20041 agcaacgttt cgctggatgt tggcggcaac gaatcactgg tgtaacgatg gcgattcagc
    20101 aacatcacca actgcccgaa cagcaactct gccatttcgt tagcaaacgg cacatgctga
    20161 ctactttcat gctcaagctg accgataacc tgccgcgcct gtgccatccc catgctacct
    20221 aagcgccagt gtggttgccc tgcgctggcg ctaaatcccg gaatcgcccc ctgccagtca
    20281 agattcagct tcagccgctc cgggcaataa ataatattct gcaaaaccag atcgttaacg
    20341 gaagcgtagg agtgtttatc gtcagcatga atgtaaaaga gatcgccacg ggtaatgcga
    20401 taagggcgat cgttgagtac atgcagacca ttaccgcgcc agacaatcac cagctcacaa
    20461 aaatcatgtg tatgttcagc aaagacatct tgcggataac ggtcagccac agcgactgcc
    20521 tgctggtcgc tggcaaaaaa atcatctttg agaagtttta actgatgcgc caccgtggct
    20581 acctcggcca gagaacgaaa ttgattattc gcaatatggc gtacaaatac gttgagaaga
    20641 ttcgcgttat tgcagaaagc catcccgtcc ctgacgaata tcacgcggtg accagttaaa
    20701 ctctcggcga aaaagcgtcg aaaagtggtt actgtcgctg aatccgcaat gataggcgat
    20761 gtcagtaacg ctggcctcgc tgtgacgtag cagatgtcgg gctttcatca gtcgcaggcg
    20821 attcaggtat cgctgaggcg tcagtcccgt ttgctgctta agctgccgat gtagcgtacg
    20881 cagtgaaaga gaaaattgat ccgccacggc atcccaattc acctcatcgg caaaatggtc
    20941 ctccagccag gccagaagca agttgagacg tgatgcgctg ttttccaggt tctcctgcaa
    21001 actgctttta cgcagcaaga gcagtaattg cataaacaag atctcgcgac tggcggtcga
    21061 gggtaaatca ttttcccctt cctgctgttc catctgtgca accagctgtc gcacctgctg
    21121 caatacgctg tggttaacgc gccagtgaga cggatactgc ccatccagct cttgtggcag
    21181 caactgattc agtccggcga gaaactgaaa tcgatccggc gagcgataca gcacattagt
    21241 cagacacaga ttatcggtat gttcatacag atgccgatca tggtcgcgca caaaacagac
    21301 cgtgccaccg gtgatggtat agggctgccc attaaacaca tgaatacccg tgccatgttc
    21361 gacaatcaca atttcatgaa aatcatgatg atgttcagga aaatccgcct gcgggagccg
    21421 gggttctatc gccacggacg cgttaccaga cggaaaaaaa tccacactat gtaatacggt
    21481 catactggcc tcctgatgtc gtcaacacgg cgaaatagta atcacgaggt caggttctta
    21541 ccttaaattt tcgacggaaa accacgtaaa aaacgtcgat ttttcaagat acagcgtgaa
    21601 ttttcaggaa atgcggtgag catcacatca ccacaattca gcaaattgtg aacatcatca
    21661 cgttcatctt tccctggttg ccaatggccc attttcctgt cagtaacgag aaggtcgcga
    21721 attcaggcgc tttttagact ggtcgtaatg aaattcagca ggatcacatt atgacctttc
    21781 gcaattgtgt cgccgtcgat ctcggcgcat ccagtgggcg cgtgatgctg gcgcgttacg
    21841 agcgtgaatg ccgcagcctg acgctgcgcg aaatccatcg ttttaacaat gggctgcata
    21901 gtcagaacgg ctatgtcacc tgggatgtgg atagccttga aagtgccatt cgccttggat
    21961 taaacaaggt gtgcgaggaa gggattcgta tcgatagcat tgggattgat acctggggcg
    22021 tggactttgt gctgctcgac caacagggtc agcgtgtggg cctgcccgtt gcttatcgcg
    22081 atagccgcac caatggccta atggcgcagg cacaacaaca actcggcaaa cgcgatattt
    22141 atcaacgtag cggcatccag tttctgccct tcaatacgct ttatcagttg cgtgcgctga
    22201 cggagcaaca acctgaactt attccacaca ttgctcacgc tctgctgatg ccggattact
    22261 tcagttatcg cctgaccggc aagatgaact gggaatatac caacgccacg accacgcaac
    22321 tggtcaatat caatagcgac gactgggacg agtcgctact ggcgtggagc ggggccaaca
    22381 aagcctggtt tggtcgcccg acgcatccgg gtaatgtcat aggtcactgg atttgcccgc
    22441 agggtaatga gattccagtg gtcgccgttg ccagccatga taccgccagc gcggttatcg
    22501 cctcgccgtt aaacggttca cgtgctgctt atctctcttc tggcacctgg tcattgatgg
    22561 gcttcgaaag ccagacgcca tttaccaatg acacggcact ggcagccaac atcaccaatg
    22621 aaggcggggc ggaaggtcgc tatcgggtgc tgaaaaatat tatgggctta tggctgcttc
    22681 agcgagtgct tcaggagcgg caaatcaacg atcttccggc gcttatctcc gcgacacagg
    22741 cacttccggc ttgccgcttc attatcaatc ccaatgacga tcgctttatt aatcctgaga
    22801 cgatgtgcag cgaaattcag gctgcgtgtc gggaaacggc gcaaccgatc ccggaaagtg
    22861 atgctgaact ggcgcgctgc attttcgaca gtctggcgct gctgtatgcc gatgtgttgc
    22921 atgagctggc gcagctgcgc ggtgaagatt tctcgcaact gcatattgtc ggcggaggct
    22981 gccagaacac gctgctcaac cagctatgcg ccgatgcctg cggtattcgg gtgatcgccg
    23041 ggcctgttga agcctcgacg ctcggcaata tcggcatcca gttaatgacg ctggatgaac
    23101 tcaacaatgt ggatgatttc cgtcaggtcg tcagcaccac cgcgaatctg accaccttta
    23161 cccctaatcc tgacagtgaa attgcccact atgtggcgcg gattcactct acacgacaga
    23221 caaaggagct ttgcgcatga ccactcaact ggaacaggcc tgggagctgg cgaaacagcg
    23281 tttcgcggca gtagggattg atgtcgagga ggcgctgcgc caacttgatc gtttacccgt
    23341 ttcaatgcac tgctggcagg gtgatgatgt ttccggtttt gaaaacccgg aaggttcgct
    23401 gaccggaggg attcaggcta ctggcaatta tccgggcaaa gcgcgtaatg ccagtgagct
    23461 acgtgccgat ctggaacagg ctatgcggct gattccgggg ccaaaacggc ttaatttaca
    23521 tgccatctat ctggaatcag acacgccagt ctcgcgcgac cagatcaaac ctgagcactt
    23581 caaaaactgg gttgaatggg cgaaagccaa tcagctcggt ctggatttta acccctcctg
    23641 tttctcgcat ccgctaagcg ccgatggctt tacgctttcc catgccgacg acagcattcg
    23701 ccagttctgg attgatcact gcaaggccag ccgccgcgtt tcggcctatt ttggtgagca
    23761 actcggcaca ccgtcggtga tgaacatctg gatcccggat ggcatgaaag atatcaccgt
    23821 tgaccgtctc gctccgcgcc agcgtctgct ggcagctctg gatgaggtga tcagcgagaa
    23881 gctggatccg gcgcaccata tcgacgccgt tgagagcaaa ttgtttggca ttggcgcaga
    23941 gagctacacg gttggctcca atgagtttta catggggtat gccaccagcc gccagactgc
    24001 gctgtgcctg gacgccgggc atttccaccc gactgaagtg atttccgaca agatttccgc
    24061 cgccatgctg tatgtgccgc agttgctgct gcacgtcagc cgtccggttc gctgggacag
    24121 cgatcacgta gtgctgctgg atgatgaaac ccaggcgatt gccagtgaga ttgttcgtca
    24181 cgatctgttt gaccgggtgc atatcggcct cgacttcttt gatgcctcta tcaaccgtat
    24241 tgctgcgtgg atcattggta cacgcaatat gaaaaaagcc ctgctgcgtg cgttgctgga
    24301 acctaccgct gagctgcgca agctggaagc ggcgggcgat tacactgcgc gtctggcact
    24361 gctggaagag cagaaatcgt tgccgtggca ggcggtctgg gaaatgtatt gccaacgtca
    24421 cgatacgcca gcaggtagcg aatggctgga gagcgtgcgg gcatatgaga aagaaacttt
    24481 gagtcgccgc gggtaaacac tgccggatgc ggcacaagtg ccttatccgg cctacaggcc
    24541 ggcaatagtt gtaggcctga taagacgcga cagcgtcgca tcaggcattg attgccggat
    24601 gcggcacgag cgccttatcc ggcctacggg tcgtgcatct gacaacaccg aatttacagg
    24661 aacacagaac atgcaaaaca ttacccagtc ctggtttgtc cagggaatga tcaaagccac
    24721 caccgatgcc tggctgaaag gctgggacga gcgcaacggc ggcaacctga cgctgcgcct
    24781 ggatgacgcc gatatcgcgc tttatcacga caacttccac ccacaaccgc gctatattcc
    24841 gctcagccaa cccatgcctt tactggcaaa tacgccgttt attgtcaccg gctccggcaa
    24901 attcttccgt aacgtccagc tcgatcctgc agctaactta ggcgtcgtaa aagtcgacag
    24961 cgatggcgcg ggctaccata ttctctgggg gttaaccaac gaagccgttc caacttctga
    25021 acttccggct cactttctct cccactgcga gcgcattaaa gccaccaacg gtaaagatcg
    25081 ggtgatcatg cactgccacg ccaccaacct gatcgccctc acctatgtac ttgaaaacga
    25141 caccgcggtc ttcactcgcc aactgtggga aggcagcacc gagtgtctgg tggtattccc
    25201 ggatggcgtt ggcattctgc cgtggatggt acccggcacg gacgaaatcg gccaggcaac
    25261 tgcccaagag atgcaaaaac attcgctggt gttgtggccc ttccacggtg tgttcggttc
    25321 cggaccgacg ctggatgaaa ccttcggttt aatcgacacc gcagaaaaat cagcggaagt
    25381 gttagtgaag gtgtattcga tgggcggcat gaaacagact atcagccgtg aagagttgat
    25441 agcgctcggc cagcgtttcg gcgttacacc actcgccagt gcgctggcgc tgtaaggagc
    25501 aaacatgatc cgcaaagcct ttgtcatgca ggtaaacccc gacgcccacg aagagtatca
    25561 gcgtcggcat aatcccatct ggccggaact ggaagcagtg ttgaaatctc acggtgcgca
    25621 taactacgcc atttacctcg acaaagcgcg taatctgctg tttgccacgg tagagattga
    25681 atctgaagaa cggtggaatg cggtagccag cactgaaatc tgccaacgtt ggtggaaata
    25741 tatgaccgat gttatgcccg ctaacccgga taacagcccg gtgagtagcg agctgcaaga
    25801 agtgttttac ctgccttaat gagttgcggt gccggaaaat atccagcacc gcgacttcat
    25861 cagaagaagt atttaaagcg aacgcgtgcg ccgtaacggt catcagtatc atcaccgtgt
    25921 tttttatcgc cgtcgctatc cagaatggag taatacgtcc cgaggtaaat attaaagttc
    25981 tccatatcca tcacgttagc gaactgataa gacgcatgaa tggtgtgaat ggtgtacgtc
    26041 ccttcatcat caatccagca gtcgttatca caaactacac cgctgaattc atcaatctta
    26101 ttgtgcgcgt agatataacc gagctcgaaa cgtttccaca gcgcgttaat cccggcagtg
    26161 aaatctttct cattattagc atctaaatag gcggtattta gattaaccac aatgccattg
    26221 tccggatccg ttttcaggcc gttccaggtc atgctcatac catagccggt acgatcggac
    26281 tgatcgacaa aattaccctt gctatcggta taaccataag cattatttac cacgttcgct
    26341 tccatcgctg cggaaacggt gaattcttcc gtcggcgacc aggcaattac tgggcgcaga
    26401 taagcaacat ttttctgctg ctccatatcg cgtccatgat aattaccgtc attatataaa
    26461 gatgttccgt cttcaagtaa cgtgtttaat tcaaaatacc agttatcgag ttgtttgctg
    26521 acgaggaaat taccgccagc gttagagcgc ccgcggccct ctttcatcat atagatataa
    26581 ccgctgccat cgtcataaag atcgttcgca gtattaccgg aatgttcaac aaaggtatcc
    26641 tgattcaatg ggaacatatc gtaggcttca aaacggccta ctttgacctt ccagtcgttt
    26701 tctttgccaa agaagaatac cgcatcatcg atatttactg aaccgtgcat atcacccagc
    26761 ggttgtgcgg agaacccggc gaaataaccg ttgtccatct ttcgcatacc gtcaaaacct
    26821 aacagaatac gaccattgag attccactgt tcattagtgg gatcgctatt cacatcagcg
    26881 ttggtcattg ccagcaggcc atgattactt tccgcatcca tattaaattc tacgtcgccg
    26941 tagattttta aatcgccata acctgaaagc aaaagattag gtgaggtggc atttttcttt
    27001 gcagattccg ccgttgcctg tccatttacc ggcgtattgc cctgagcggc cttaatttcg
    27061 cggatctcag cggcttgttg tgtctgcagc tgtttaacct ggctctctgc ttttgccgcc
    27121 cgcatttctg ccgcttccag gcgttcttcc agcaatgcca tgcgttgttc cagcattaaa
    27181 tgctgtgttt ttgccatacc ggtactacat gccaataata aaccgatggc taaagataat
    27241 gtcgattttt tcattattta cgttccctga aataaaggag accctacgct ttccaaaagg
    27301 aatacgtata tagagaaata taaggtgtgc actttcgaat ttttaatcca acttcagcag
//
LOCUS       DBFIFM010000043        27151 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.043, whole genome shotgun sequence.
ACCESSION   DBFIFM010000043 DBFIFM010000000
VERSION     DBFIFM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.043"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            63..1079
                     /locus_tag="N7C74_001630"
     CDS             63..1079
                     /locus_tag="N7C74_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HGU2431523.1"
                     /translation="MKTVNVALLALIISATSSPVVLAGDTIEAAATELSAINSGMSQS
                     EIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTPTVQTDPDAGQKTVAATGD
                     VQTTARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTLAATGDTQNTAHYQEMINA
                     RLAAQNEANQRTATEQGQKMNALTTDVAVQQQNERTQYDKQMQSLAQESAQAHEQIDS
                     LSQDVTQTHQQLTNTQKRVADNSQQINTLNNHFSSLKNEVDDNRKEANAGTASAIAIA
                     SQPQVKTGDVMMVSAGAGTFNGESAVSVGTSFNAGTHTVLKAGISADTQSDFGAGVGV
                     GYSF"
     gene            1164..1586
                     /locus_tag="N7C74_001631"
     CDS             1164..1586
                     /locus_tag="N7C74_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU2431524.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPTSRIADCQAQGVSHDTCYLAE
                     QQRQAAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNKDATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            1583..1786
                     /locus_tag="N7C74_001632"
     CDS             1583..1786
                     /locus_tag="N7C74_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2431525.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGIH"
     gene            complement(1790..2197)
                     /gene="cueR"
                     /locus_tag="N7C74_001633"
     CDS             complement(1790..2197)
                     /gene="cueR"
                     /locus_tag="N7C74_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HGU2431526.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(2194..3369)
                     /locus_tag="N7C74_001634"
     CDS             complement(2194..3369)
                     /locus_tag="N7C74_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HGU2431527.1"
                     /translation="MNGQLQYNRGLTEPRLPRSALAVRVTAVMLLCFLGWAWYFQLDE
                     VTTGSGTVEPSGREQVVQSLEGGILYHLDVKVGDIVEQGQPLAQLNRTKTESDVQEAM
                     SRLYAALATSARLRAEVSNKPLVFPDELNKFPELIESETALYNTRRDGLNKATTGLTQ
                     GISLVNRELAMTQPLVKQGAASSVEVLRLQRQANELENKLSDVRTQYYVQAREELAKA
                     NAEVETQRSVIRGREDSLTRLNFTAPVRGIVQDIDVTTVGGVIAPGGKLMTIVPLDEQ
                     LLIEAKISPRDVAFIHPGQKSLVKITAYDYSIYGGLPGEVAVISPDTVQDEVRRDVYY
                     YRVYIRTFSNHLENKSKQQFPIFPGMVATVDIRTGKKSVLDYLLKPFNKAQEALRER"
     gene            complement(3366..5528)
                     /locus_tag="N7C74_001635"
     CDS             complement(3366..5528)
                     /locus_tag="N7C74_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HGU2431528.1"
                     /translation="MKKNAQSVEAWLEAMIAVARYYRLDFSQENVRATVNWERDSKRE
                     ELLTDMARQLGMGLRLVEFSADSLNPWRLPLIAVFDNQQIGVITRRDNHDNISVQFSG
                     DEGLETTLNVADIEDKIVELALLRPLSAIPDARVDDYIRPYQANWFWSLSLKDWRRYG
                     DIMLASLVANVLALAAMIFSMQVYDRVVPAQSYPTLWVLFAGVMMAILFEFCMRMVRT
                     HLSDVIGKRADLRISDRVFGHALRLKNNVRSKSTGSFISQIRELESVRELITSTTIGA
                     VADLPFFLLFVFILWMIGGWLVLVVLLALLLLVIPGLLVQRPLARLANEGMRESAVRN
                     ATLVEAVQSIEDIKLLRAEQRFQNQWNHTNDVASSISMKQRFLTGLLLTWTQEVQSIV
                     YVVVLLVGCFMVMNGDMTTGALVGTTILASRTIAPLSQISGVLSRWQQAKVARNGLDE
                     LMKRPVDQPEHGKLVHKAVLHGNYQFSNAVFYYDEEEKIADVAIGKLNIQAGEKIAIL
                     GRNGAGKSTLLQMLAGMRIAQQGQVLLDNISIGQLDPADLRRDMGLLSQTGRLFFGSL
                     RENLTMGMPEASDEDIERALTLSGALPFVQKQKNGLNYMIQEGGFGLSGGQRQTLLLA
                     RLLISQPNIVLLDEPSASLDEMAEAYLIEQLKQWIGHRTLIIATHRTAMLQLVDRIIV
                     MDQGRIVMDGAKEAILREQGEPTARRVVLQEKNKGSAA"
     gene            complement(5532..21253)
                     /locus_tag="N7C74_001636"
                     /pseudo
     CDS             complement(5532..21253)
                     /locus_tag="N7C74_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308569.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(21290..25675)
                     /locus_tag="N7C74_001637"
     CDS             complement(21290..25675)
                     /locus_tag="N7C74_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HGU2431529.1"
                     /translation="MSLIIDVISRKTSVKQTLINPGDVTVVIYEPSVVQVHAQASAVA
                     RYVREGNDLLIYMRDGTVIRCNGYFLQAANTAEQSELVFADGQQLTHITFADTAAGGL
                     APVELTAQTTAIESIAPFLDTVAQTSAFPWGWLAGAAVGGGALGALLASGGDGDSKTE
                     VINNPTPPAEPGNATPSFLVTDNQGDQRGILATNDITDDTTPTFSGSGQAGATIQIKD
                     SNGNTIASTQVDNNGHWSVSLPTQSAGEHTWSVVQIVGSTITDAGSITLTIDNSQASV
                     QVATTAGDNIINASEQAAGFTLSGTSSHLAQGTELTVTLNGKTYTTSVGANGAWSVQV
                     PTADAQALGEGNQAVLVSGKDATGNTVTGAQLLTVDTQPPTLAINTIAQDNIISAAEH
                     NVALVLSGTSNAEAGQTVTLTVNGKSHTATVGSDGTWQVTLPATEVQALAEGNYAVNA
                     SVSDRAGNTTSHSANFTVDTSAPVVSVNTVAGDDILNNAEQAVAQIISGQVSGASPGD
                     TVTVKLGTHVLTGIVLADGSWNVALDPAVTRTLDRGANTIFVTVTDAAGNTGAASRAI
                     TLVGVSPLITINTVSGDDIISGAEKGAPLTLTGSTQQAETGQTVTVTLAGQSFTTTVQ
                     ADGSWSLTVPAAAMGNLPDGAVAITASVTDLSGNTGNTSRTITVDSQAPALSIDPLTA
                     DNIINAAESGQDLPITGTTDAQPGQTVTVTLNGQTYQGVVQPDGTWSVTVPAANVGAL
                     ADGNATVTASVNDVAGNPSSVSRVALVDATPPVVTINPVATDNVINTPEHAQAQIISG
                     TVTGAQAGDIVTVTLNNVDYTTVVDGSGNWSLGVPASVVSGLADGSYPVSVSVTDKAG
                     NTGSQSLTVTVNTAAPLIGINSIAGDDVINASEKGADLQITGTSDQPVNTAITVTLNG
                     QNYTTTTDASGNWSVTVPASAVTALGQANYTVTAAVTSDIGNSATASHNVLVDSALPG
                     VTINPVATDDIINAAEAGVAQTISGQVTGAEDGDTVTITLGGNTYTATVGSNLTWSVD
                     VPAADIQALGNGDLTVNASVTNQNGNTGSGTRDITIDANLPGLRVDTVAGDDVVNIIE
                     HGQALVVTGSSSGLAESTPLTVTINNVEYTTAVQADGSWSVGVTAAQVSAWPAGTVNI
                     AVSGESSAGNSVSITHPVTVDLTPAAITINTIATDDVINAAEKGADLTLSGTTTNVEP
                     GQTVTVTFGGKNYTASVASDGSWTATVPAADLASLPEGSASALASVSNINGNSASAVH
                     NYSVDSSAPTIIINTVASDNIVNASEADAGVTVSGSTTAEAGQIVTITLNSPTVQTYQ
                     ATVQADGSWSINIPAADLEALTDGSHTLTATVNDKAGNPASTTHNLAVDLTVPVLTIN
                     TIAGDDIINATEHGQALVISGSSTGGEAGDVVTVTLNSKTYTTTLDASGNWSVGVPAA
                     DVTALGSGPQTVTATVTDAAGNSDN"
     gene            complement(25778..27133)
                     /locus_tag="N7C74_001638"
     CDS             complement(25778..27133)
                     /locus_tag="N7C74_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HGU2431530.1"
                     /translation="MARFQFKNRKNNGLIFFISFMVMGEAAIAAPLPQWANAPAVTPV
                     AQLSLQESILRAFARNPGVTQQAAQIGIGEAQIDEAKSAWYPHVGLTGNAGPSRQTDS
                     SGRLDNNVSYGITLTQLVYDFGKTNNDINLQTAARDSYRFKLMATLTDVAEKTATAYM
                     EVSRYQALCDAAQRNIHSLENVYNMAALRANAGLNSSSDELQAQTRIAGMRSTLEQYQ
                     AQMASAKAQLAVLTGVQPEAIAAPPAELAEQPVSLKNIDYQSIPLVLAAENLRQSAQY
                     GVEKTKAQYWPTLSIQGGKTRYQTSDRSYWDDQLQLNVNAPLYQGGAVSAQVQQAEGQ
                     QKISASQVEQAKLDVLQRASVAYANWTGARGREEAGLAQSESAHKTRDVYQNEYKLGK
                     RSLNDLLTVEQDVFQAQSAEINANYDGWVAAVNYAAAVNNLIPLAGIKQGLYNDLPDL
                     K"
BASE COUNT         5053 a         8008 c         7875 g         6215 t
ORIGIN      
        1 aacaatatta tttgtttctt atagaaacat taatacgact tattttgaac aagagaaaat
       61 gaatgaaaac tgtaaacgta gctttactgg cactcataat ttcagcaaca tccagccctg
      121 ttgttttagc tggtgatacc attgaagcgg cggcaacaga gctttcagcc attaactctg
      181 gcatgtcgca atcggagatt gagcagaaga ttacccgctt tttagaacgc acagacaaca
      241 gccccgctgc gtatacctat ttgactgaac atcactacat cccttctgaa acacctgata
      301 ccactcagac tcccactgtc cagacagatc ctgacgcagg acaaaaaacc gttgccgcta
      361 caggtgatgt acagacaact gcccgttatc agagcatgat caacgcccga cagtctgcgg
      421 taactgacgc ccagcaaacg caaattacag agcaacaggc gcagatcgta gccacacaaa
      481 aaacgctcgc cgcgactgga gatacgcaaa ataccgcgca ttatcaggaa atgattaatg
      541 ccagactggc ggctcaaaat gaggctaatc agcgcaccgc cactgaacaa gggcagaaaa
      601 tgaatgcgct gacaaccgat gtggcagtac aacagcaaaa tgaaaggact caatacgata
      661 aacaaatgca aagtctggcg caggagtctg cccaggcaca tgaacaaatt gacagcctgt
      721 cacaagacgt aacccaaacg caccaacagt taaccaacac ccaaaaacgg gttgcagata
      781 acagccagca aattaacacg ctcaataacc atttcagttc gctaaaaaac gaagttgatg
      841 acaatcgtaa agaagccaat gcgggaactg catctgccat cgctatcgcc tcacaaccac
      901 aggttaaaac cggtgacgtg atgatggtgt cagcgggagc gggaaccttc aacggtgaat
      961 ctgcggtgtc tgtcggaaca tcatttaatg ccggaacgca tacggtactt aaagccggta
     1021 tttctgcgga tacacaatct gatttcggcg caggtgtcgg cgtgggatat tcgttctaat
     1081 atttcaatcc tcaatataaa taagagcaag gaagcttgcc gggttcacct cttcattaat
     1141 ttgtacatta tttaaggtta acaatgatga atagctccat taaatcgttt tccctgctgg
     1201 cggttatatt actggctggc tgtagttcac ccacttcccg catcgcagat tgccaggcgc
     1261 agggcgtcag tcatgacacc tgttacctcg cagaacagca gcgtcaggcg gctattttaa
     1321 gtgcatccga ggcacaggca tttaaaaatg cagaagccgc acaacacgcc caggcggcaa
     1381 agaaagccat ttataaagga tttggcatga cctttagaat gagcagtaaa aactttgctt
     1441 atctcaatga ttcattatgt gcaattgatg aagacaataa agatgccact gtttatcagt
     1501 caggtctata taacgtcatt gtttatcatc acacaggaaa agtcgcctta atgaaagaag
     1561 gccagtttgt gggttattta aaatgaagga gcaaaggaaa atacccctga cgcatattat
     1621 gattatcggt gcgtttattt ttgccttctt gcaagtagta ttattagcct ccctggttca
     1681 cgctgtgaat gttaacaacg aaatccagga aggcttattt cagtcggggc gcattatggt
     1741 agaaagtttg cagcatattc tttcggtgca aacggggatt cactgatttt caccccgccc
     1801 gatgatgaca gcagccggag agattttcga taatcgggca gtcggcgctg tcatcaccgg
     1861 ggcaggcatt cgccagcgcc agtagctggt tgcgcataga ttgcagttct tcgatatgcc
     1921 gttcaatctc cgccaccttc tccagcgtgc gacgtttgac gtcggcactg tgacgctgcg
     1981 ggtcgttaaa cagattcacc agctcgccgc tctcttccag gttaaagccc acctggcgcg
     2041 cctggcgcag taaggtcaat tcgttgagat gctgctgcgt gtaggttcga taaccatttt
     2101 cgctgcgcat cggcggcgtc accagcccct tctcttcata gaagcgaatg gctttgctgg
     2161 tcaggccggt aatttttgct acatcgctaa tgttcatcgt tcgcgcaacg cctcctgtgc
     2221 tttgttaaag ggtttcaaca gataatccag cacgcttttt ttgccggtgc ggatatcgac
     2281 cgtcgccacc ataccgggga atatcggaaa ctgctgtttg cttttgtttt ccagatggtt
     2341 gctgaaagtg cgaatgtaca cccgatagta ataaacatcg cgccgcactt catcctgcac
     2401 ggtgtctggt gatatcaccg ccacttctcc cggcaggccg ccgtagatgg agtaatcgta
     2461 ggcagttatt ttcaccagcg atttttgccc cgggtggata aacgccacat cgcgcgggga
     2521 gatttttgcc tcaatcagca gttgctcatc cagcggcacg atggtcatca gtttaccacc
     2581 gggggcgatg acgccgccaa ccgtggtgac atcaatatcc tgaacaatgc ctcgcaccgg
     2641 cgcggtaaag ttgagcctgg tgagagagtc ttcccgtcca cggatcaccg aacgctgggt
     2701 ttccacttcc gcattggctt ttgctaactc ttcgcgcgcc tgaacgtagt attgcgtgcg
     2761 cacgtcgctg agtttgtttt ccagttcatt ggcctggcgt tgcaggcgca gtacttcgac
     2821 gctgctcgct gccccctgtt ttaccagcgg ctgggtcatc gccagttcgc gattgaccag
     2881 gctaatcccc tgcgtcagcc cggtggtggc tttgttcaac ccgtcgcggc gagtgttgta
     2941 caacgccgtt tccgactcga taagttcggg gaatttattc agttcgtcag ggaagaccag
     3001 cggtttattg ctcacttccg cccgcagccg cgccgaggtt gccagcgccg cgtacagccg
     3061 cgacatcgct tcctggacgt cggattccgt tttggtgcgg ttaagctgcg ccagcggctg
     3121 gccctgttcg acaatatcgc caactttcac gtcgagatga tacagaattc cgccttccag
     3181 cgactgcacc acctgttcgc gcccggaagg ttctaccgtg ccgctgccgg tggtgacttc
     3241 atcaagctgg aagtaccacg cccagccgag aaaacagagc agcatcacag cggtaacgcg
     3301 taccgccagc gccgaacgcg gcaggcgggg ttcagtcaga ccacggttat attgcagttg
     3361 tccgttcatg ccgcagaccc cttattcttc tcctgcaaca ccacccgtcg cgctgtgggc
     3421 tcgccctgtt cacgcaaaat ggcctctttc gcgccgtcca tcacaatccg cccctgatcc
     3481 atcacgatta tcctatccac cagttgcagc atcgccgtgc ggtgagtggc gataatcagc
     3541 gtgcgatgcc caatccactg tttcagttgt tcgattaaat acgcttctgc catttcgtcc
     3601 agtgaggcgc tgggttcgtc gagcaggacg atattcggct gagagatgag tagccgcgcc
     3661 agcagcagcg tttgccgctg accaccagag aggccaaatc ccccttcctg aatcatgtag
     3721 ttgaggccgt ttttctgctt ctgcacaaag ggcagcgctc cgctgagggt taacgcgcgt
     3781 tcgatatctt catcgctggc ttccggcatc cccatggtga ggttttcccg cagcgaacca
     3841 aagaacagtc gcccggtctg gctgagtagc cccatgtctc gccgcagatc tgccggatca
     3901 agctgcccga tactgatgtt atccagcaac acctgcccct gctgggcgat acgcattccg
     3961 gcgagcattt gcagtagcgt cgatttaccc gcgccgttgc gcccgagaat ggcgattttc
     4021 tcaccagcct gaatgttgag tttgccaatc gctacatcgg caatcttctc ttcttcgtcg
     4081 taataaaaca ccgcgttgct gaactgataa ttaccgtgca gcaccgcttt atgcaccagt
     4141 ttgccgtgtt ccggctgatc caccgggcgc ttcatcagtt catcaaggcc gttacgtgct
     4201 actttcgcct gctgccagcg cgagagcacg ccggatatct gcgacagcgg cgcaatggtg
     4261 cgcgaagcca ggatcgtcgt cccgaccagc gcgccggtgg tcatgtcgcc gttcatcacc
     4321 ataaagcagc ccaccagcag caccaccacg tagacgatgg actgcacttc ctgggtccag
     4381 gtgagcagca gcccggtgag aaaacgttgc ttcatgctga tggacgaggc cacgtcgttg
     4441 gtgtggttcc actgattctg aaaacgctgt tcggcgcgca gcagtttaat atcttcaatc
     4501 gactgcacgg cttccaccag cgtagcgttg cgtaccgctg actcgcgcat tccttcgttc
     4561 gccagccgcg ccagcgggcg ttgcaccagc aggccgggga tcaccagcaa cagcagcgcc
     4621 agcaaaacca ccagcaccag ccagccgccg atcatccaca aaataaagac aaacagcagg
     4681 aagaatggca ggtccgccac cgcgccgatg gtggtggacg taataagctc ccgcactgat
     4741 tccagttcgc ggatctgcga gataaacgat ccggtggatt tcgatcgcac gttgtttttc
     4801 agccgtagcg catgaccaaa aacgcgatcc gaaatacgca ggtctgcccg ctttccgatc
     4861 acatcagata gatgcgtacg caccatgcgc atacagaact caaacaggat cgccatcatc
     4921 accccggcga aaagcaccca tagcgtcggg tacgattgcg ccggaaccac ccgatcgtaa
     4981 acctgcatcg agaaaatcat cgccgccagc gccagcacgt tcgccaccag tgacgccagc
     5041 ataatatcgc cgtagcgccg ccagtctttt agcgaaagac tccagaacca gttcgcctga
     5101 tacgggcgaa tatagtcatc cacccgcgcg tcagggatgg cgctgagagg gcgcagcagc
     5161 gccagttcga cgattttatc ttcaatatcc gcgacgttaa gtgtagtttc cagcccttcg
     5221 tcaccgctga actgaacgct gatattgtca tgattgtcgc gacgggtgat cacgccaatc
     5281 tgctgattat caaacaccgc gatcagcggc agacgccagg ggttaagcga atcggcggaa
     5341 aactccacca accgcaaccc catccctaac tgccgcgcca tgtcggtgag taactcctcc
     5401 cgcttactgt cgcgctccca gttgacggtc gcgcgcacat tctcctgaga aaaatcgagc
     5461 cgataatagc gcgccacggc gatcatcgcc tccagccagg cttccacgga ctgggcattt
     5521 tttttcatta tctacaccac catcaactga tgattcgcca gcaaggtggc gaggtcggtg
     5581 tgcacgcccg tcagcgtgac cacgttagtt gtcgcaaaag tgccgcccgt gccgtcgcgg
     5641 tcgatctgca cgatggtgtc gctgccgctc tgggtgactt tgacaaagtc accgatgttt
     5701 ccggcctgcg catccagcgt tgccacgccg ttgacgtagc tggctttgcc gttgccggtg
     5761 tagccgctgc cctgtaacag ttcacgaata tcaatgcgat cggtgtcggc ggtgccttcc
     5821 catgtcccta ccgtaaagcc attcaccacg tctgagccgt tgccgccagt ggcgtcgctg
     5881 gcgttgagca gtttatagag caaggtgtcg tggccgccgt tgccgatgtt gagcgtgtcg
     5941 ttgccgccgc gcccttccag ttggttatcg ccgctgctgc cggtcagaat gtcgttaaaa
     6001 ttcgagccgg aaatgccttc gatattggta aatgtcgagg tgttaaaacc ggtggcctgc
     6061 gcggcagtgc tgcttaagtt agccgtcacg cccaccgtcg aaagccggaa atcaaccacg
     6121 tccatcccgc cgttcgccag ccacgttccg gtgccggagc tgtacaccca gccgccggaa
     6181 ccgtcgtaag tgtcggtgcc tgcggtggcg aagaaggtgt cgttatagcc ggtgccgacg
     6241 aacttgccgt tgttgctcgc ggtacccgct tcagcgctga gatcgtaagc gtgagtggca
     6301 tcggtggcct gtaaaccgcc ccagctggtg ttgaccgtct ggtcgatatt gctggcggtg
     6361 gtgccggtgg atttgatcag caccgcgttg taggtttctc cggcattcag cccgtagaag
     6421 ttgaccagcg ccgaggtgtc gttaaccccc atacccgact gcgggttgat gatttgcgtg
     6481 gcgaccagca ctcccgccga gttgtacagc ttcacggtat tgccgtaata gacgttaatg
     6541 ccgttgggat cggtgatgcg caggtgtagc gaagtgccgt agctcaccgt gttggtgttg
     6601 cggctcagga acacgctgcc cgactgtttg gacaccagca catccaccgc gccgtcccag
     6661 tcgtagtcca ttgccgccac gccgctggtg gtaccgctct ggctgccgcc gagggccgat
     6721 tgcgtccagt tgctggcgtt gctgacgtta gtataaagat acgactgccc ggtctgggcg
     6781 atacgggcga tatccatcag gccgtcgtgg ttccagtcca ccgccaggct aaactggctg
     6841 gcgtaggtgg ttgccgttgc gcccaccgcc accgggctac ccagtacgcc gttggtgttg
     6901 aataacagcg agccgtagtt agctcttccc tggctggcgg gcaggaagag atccatatcg
     6961 ccgtcaccat cgaaatcggc ccaggtcatg gaaacgctgc tgctcatagc gccactgccc
     7021 gccccgctca ggaaggtatt ggtggtgttc tgcccccaga caaacgtccc attcccctgg
     7081 ttgatgagcg aagacagcgt gtaatagttg ttcaggttat aggtgtgctg aaccaggtcg
     7141 atcctgccgt cattgttcag atcgacgcca gaaccttcgt tgagcgaaag ataccccgtc
     7201 accgccccgc ccaccgtggc gctaccgccg ctgttcgacg tggtggagtt gcctaccagc
     7261 gtgcctgcgt tgttccacag gaaggtgttg gagtccggcc cgccagcgtc accaatccag
     7321 aagtcgagat agccgtcgcc ctctttatca aatgccacaa tcgagccgta ccacgtcagg
     7381 gtgccgacgg tgacctgaat agcgctggaa aaggtgccat cgccgttatt ggtaaagccg
     7441 ttaatatagc ccgtaccgta gtcatcacgg gtgataaaca gatcgcccgt gccgttacgg
     7501 tcgtaatcgg caatggagct ggtggcgtag ttgttgccgc tctgatacag cgtcagcgcg
     7561 gtcttcgacc aggtgagtgg gtcagtcgat tgcattacct gctggtttgc cagcaccgtc
     7621 cacatcccgt gcgagtcgag gccgtaggtc agcccccagg cggtggtttt gctggcggta
     7681 gtccaggtcg gtgtgtaatc aatcgccgcg ttaaccgtca ccgtgccgtt gctaatattg
     7741 gcgttattgc cgttacccgc cgaacttttt acctgcgcag taacggtata ggcggtcgcg
     7801 gaaactgtca gcgcatcggt atccggcaac tgtacatacc aggtgttgtg cgccggatcg
     7861 accactaccg cgccgcccgg ttcagaggtg taggttttgc cgttgatcac cacttcaaca
     7921 tactgcccac tggcaagcgc ggcggtgatc accccgctat agtatcgcgc gtggtctggc
     7981 tggtgatttg cgccagcgtg gtcggaatag aggtatcgac cgtcaggaca aaatcactgg
     8041 aggaggtgat attccccgcc aaatccacca ctcgcgcgct gtaggtatag gcaccgctca
     8101 ccagcccgct gtcgctgtag gtccagttca gagcgccgac catcgtcacc gcccctaaca
     8161 gcagcccgtt acggtagagg taaaccactt caccgctggc aagcggcgcg gaaagtacac
     8221 cgttcagcag cggcgtagtg tcgtcggtgg cctgcgaact gcttaatgtc ccctgccgct
     8281 gcccgacatc atcggtatac gacacaatcg ttggtgtggc ggtggggtta atcgtatcga
     8341 ccaccaccgt tttcgacacc accgggccgg tgttccccgc cagctccagc acccgcacct
     8401 gataaacgta gctgccgtcg gtcagcgtgc ggctgtcggt gtaagtccac tgtgtaccgt
     8461 tggtggtgag cgttgtccag gtcgcgccgc tatcaaggct aatctgcgcc acttcgccgc
     8521 tggcaagccc cgcccccagc gaaccggtca gcgtgagcat ggtgtcgctg gtgataaagt
     8581 cagttgccga actccccgtg tcggtgctga tgctggcgat ggtgagcacc tgcgccgggt
     8641 taacggtatc gatcagcgcc gactggctgc tcgttgcgcc aacgttgccc gccagatcga
     8701 ccacccgcac ctgccaggtg gtggtgccgt tggtgagggt tcgtccgtca acgtaactcc
     8761 agttcaggct gtctgcggcg acggtcacgt tcacccaggt tgcgccgttg tcggtactga
     8821 tttgcgcgaa ctcattagcg ccgagcgccg cgcccaatac gccgcgcacg gtaagcgtgg
     8881 tgtcgctggt aataaaatcg ctgctgctgg ttccggtgtc atcgctgata ccggtaatgg
     8941 tgatactttt cgccgcttcc gggctggtgg tgtcgaccac cacgttttgc gttgcggtct
     9001 gcccaacgtt ccccgccaga tccaccaccc gcaccgtgta gttccaggtg ccgtcagtaa
     9061 gcgtgtgacc atcggcatag ctccagctgg tgccaacgac ggttaacgta gtccaggtga
     9121 cgccgccgtc gaggctgatt tgcgcgaact caccggcaga aagcgtcgcc cccagcgtgc
     9181 cgctcaccgc aagcgtcgtg tcgctggtga caaaatcgtt agtgatcaag cccatatcgg
     9241 tggtgatcgc gctgatggcg atggttttca ccgcgggatc tggcgcggta gtgtcgatca
     9301 ccacattctg gctgtcggtc gcgccaacgt tgcccgctgc gtcaatcacc cgcacctgat
     9361 agagatagtt gccatcggtc agcgtgcggc tgtcgttata gcgccaggtc gtgccggtaa
     9421 cggtaagcgt ggtccaggtt gtgccgccgt cgatacttat ttgcgccgtc tcgttactgg
     9481 caagcgcggc ggtgagcgag ccgttgacca ctaccggact gacgctggtg ataaagtcgc
     9541 tggcgctaag gccagtgtcg gcttgcaggg aatcgatatt aatgcccatc gccggagcgc
     9601 tgagatcgac ggtaatggtg tgattactgc taccggtgtt gccaatggca ttgctcaccg
     9661 aggcgttaat cacgtaggcg ttgccgtccg ccagcgcggt gacatcggcg ctgccgaggg
     9721 tgtagctcca gctaccgccc gtctgcaccg tggtggtgta ggttttgccg tttaaggtca
     9781 gggtcagcgt ctgcccgacg ggcgcggtgg tggtgccgct gatcaccagc gatgagccgt
     9841 gttccgctgc attcaccaca tcgtcgcccg caaaggtgtt gatggtgaca gtaggtacat
     9901 cgccgttcag cgtcacgcca cggctggcgc tgccggggtt tccggcctga tcgctaaccg
     9961 tcgcgctaat ggtgtagctg ccgtcgctta atccggcaaa ctgctgcgcc ggaataaaca
    10021 ccgaccagct tccgccgctg ccaaccgttg cgctccaggt ttgcccgtta aaggtaacgg
    10081 tcaccgtctg gcccacttcg gcggtggtgg tgccgctaat ggtctgcccg gcaagctgct
    10141 cggcgttgtt gataatatcg tcacccgcca ccgtggcgat ggtgaccgtt ggcgcaacgg
    10201 tatcgacggt cagggcgtgc gtggcctgtc cggggttacc ggcgcgatcg ttcacgctgg
    10261 cggtaatggt ctgcgcgccc tgcccgagcg cggccagatc caccgcaggc acgttcagcg
    10321 cccaggtacc attggcggca accgtggcgg tataggtttt gccgccaaag gtgacggtga
    10381 ccgtctgccc cacttccgcg ctggtcgagc cgttaagggt taacggctgc tgctgttcgg
    10441 cggcgttgag gcggtcgtcc gtcgaaacgg tgttaatgct gataaccggc gcggtgagat
    10501 ccaccgccac gctgcgcgag gccgtggcgc tgtttccggc actgtcctga gcggaggcgc
    10561 taaccgtgta gctggtgcca tcactgagtg ccgcaacatc cgccgcaggc acgttcacgc
    10621 tccagctccc gttgctctga atggtggcgc tgtagccttt gccgttaagc aacacggtca
    10681 ccactgtacc tgtcgcgaaa ttagtgccag tgccgctcag ggtcagcgca ctgcccgctt
    10741 cggcggcgtt aataaggtta tcgccgctga tagtgctgac cgagagcgac actgccgccg
    10801 tgttcacctg tacgttgtgc gtctgcgtgc tgctgttgcc tgcgctatcg gtaatggtgg
    10861 cgctgatggt caccgtgcca tccgcaaggc cggaaatcac gctggcaggc acgccgacgc
    10921 tccagttgcc gctggcatcg gtgctggtga catactgctg cccggcgatg gtcaccacca
    10981 ggcggtcgcc cgccaccgcg cccgtggcgg tgccgctgat aatctgcgcc tgaatgtgtt
    11041 cgacgttgtt aatcacatca tcgcccgcca cggtgttaaa actgatcacc ggcgcagtgg
    11101 tatcgacggt cacgcctttg ctggcgctgc caaggttacc cgccagatcg ctgaccgtgg
    11161 cggtcagggt gtagccgtta ttagcaagtg cggttaagtc gctggcgggc agcgttaaac
    11221 tccagctgcc gtctgtctgc accgtggtct ggtagttagt gccgttaagc gtgacggtga
    11281 gcgtctgccc ggtttgcgcg gtgctggtgc cggacaccgt tacgcccgcc gccgcttcgc
    11341 tggcgttgat gatgttatcg ctggcaatgg tgttgatggt caccgttggc gcggcgctat
    11401 cgacgctgta ttcgtgcgtt gctgtggcgc tgttgccgtt gacgttagtc acccgcacct
    11461 gggcgctggc atcgccatca cgcagggccg ccaaatccgc cgccggaacg gtggtgctcc
    11521 agctaccgtt tgccgccacc gtggtggtgt aggttttgcc gccaaaggtg acggtgacgg
    11581 tttgccccgc ttcgacgccg gacgtgctgc cggagagcgt taacgccgcg cctttttcgg
    11641 cggcgttaat cgcatcatcg ctggtgatgc tgttcatgct gatggcgacg gcggtgagat
    11701 caacagtcag cggatgggta atgctggttt gcgttccggc actgttcgcc ccgctaacgg
    11761 taatattcag cgttcccgca ggccagttac tgacatccgt cgcaggaacg ccgacgctcc
    11821 agctaccgtt cgccaacacc gtggcggcgt aagtttggtt gttaatcacc accgtcaacg
    11881 ccgtgcctgc ggcaaagccg gagctgctgc cggtgatcac ctgcgcctga gcgtgttcga
    11941 tgatattgac cacatcatca cccgcgacgg tatccacccg caggccaggc agactggcat
    12001 cgatggtgat atcgcgcgtg ccgctaccgg tgttgccaac ggcgttggtc accgaggcat
    12061 taaccgttaa ctcaccgtta cccagagcct gccagtcggc ggcaggtacg ctcacattcc
    12121 agcttaaatc agcctggacg gtggcggtgt aagttttacc gcccagttca acggtgacgg
    12181 tgctgcccgc cgccgcaccg gttacctggc cgctgatagt ttgcgcgctg cctgcctctg
    12241 aagcgttgat aatatcgtcc gtcgccaccg gattaatggt gatgcccggc agcgcggtat
    12301 taacctgcac gttatggctg gcggtgctgc tgttaccctg agcattggtg acgctcgccg
    12361 tcacgctgta ggtcgcttcg cccagcgcac tgaccgccga ggcaggcacg gtaacactcc
    12421 agttgccgga ggcatccgtg gtggcgctgt agttttgtcc gttcagggtg acggtgatcg
    12481 ccgtacccgc aggctgatcg ctggtgccag aaagcgccag atcagatcct ttttccgtcg
    12541 cgttgatcac atcatcgccc gcgatggtgt tgatgctaat cactggcgcg ctgaggctga
    12601 cggtcacggt gcggctggcg tcgtcgctgt tgcctgcccg atcgctgacg ctggcggtga
    12661 tggtctgcgc accgctaccc agcgccgtaa catctgccgc cggaacgcca acgctccagt
    12721 tgccggaggc atccagggtg gtggtgtagg ttttgccgtt gagcacaacg ctcaccacat
    12781 cgcccgcttc cccgccgcta ctggtgccgg agatcaccag cgcctgcgca tgttcggcat
    12841 cgttgatgat gtcatcgccc gccacggtgt taatggtgag aaccggcgcg gtggtgtcta
    12901 ccgccagatt atggctggcg ctgccggggt tgcccgcttt gtcattgacc gtggcgctga
    12961 cggtgtaatt gcttgcgttg agcgcgccca gggctgaagt gggaacgctg acgctccagc
    13021 taccgtccgc ctgcacagtg ccggtgtagg ttgcgccgtt aagggtgacg gtcaccgtct
    13081 gcccggtttc ggcggtgctg gtgccgctga tggtgagcgg gcttccggct tctgcggcgt
    13141 taaggatatc gtcgctggcg atggtgttaa tggtgagcgt cggcgcgctg gcgtcaacgc
    13201 tatacgcatg ggtcgccgag gcactgttac cgctcgcgct gctaacgctg gcctgcacat
    13261 tcgccgcgcc ctctggcaga gttgccagat ctgccgccgg aacgtttaca ctccagctcc
    13321 cgttggcggc gacgcttgca gtgtaggttt taccgccaaa agtgacggtg acggtttgcc
    13381 cgctctcaat cccgctggta ctgccggaaa gagtcagatt ggtgcctttt tctgcggcgt
    13441 taatcacgtc gtcgctggca acggtgttga tgctgattgc caccgccgtt aaatcgacgg
    13501 tgaacggatg gctgacgctg actgggttat tggcgctgct ttgcccgtct acctccacgg
    13561 ttaacggccc cgcaggccag gcgctgacgt ttgccgccgg aatgccaacg ctccagcttc
    13621 cgtccgcctg caccgtggcg gaatacgcca cactgttgat ggtaaccgtc agcacagcac
    13681 ctgcattcag gccgctactg ccgccggtga tcaccagcgc ctgcccgtgc tcgatgctgt
    13741 tgacgatatc atcgcccgcc acggtatcta cgcgcagccc cggcaggttg gcatcaatgg
    13801 tgatatcccg cgtgccgctc ccggtgttgc cattagcgtt ggtgaccgag gcggtaatgg
    13861 tcaaatcacc attgcccaac gcctggagat cggctgtcgg aacgctgacg ctccagctta
    13921 agttgctctg taccgtagcg gtgtaagtgt tcccgcccag cgtcacggtg accgtatcgc
    13981 cagcggcggc gcgagtcacc accccgctga tggtttgatc ggcccccgct tcggcggcgt
    14041 tgataatatc gtcgcctgcc acggtgttga tggtgacgtc cggcaggccg ctgtcgacca
    14101 gtaaattcgc ctggctgctg gcggtgttgc ctgctgcact ggtggcgctg accgtatagt
    14161 tggcctgacc taatgccgcc agatcgctca ccggcacggt taaggtccag ttgcccgatg
    14221 cgtcggtggt ggcgctgtag ttgtgaccgt tgagcgtgac ggtgacctgc gcgcccgtcg
    14281 ccagccccgt actgccgccg ctgatggtca acggctggcc tttttcatcg gcgttcagga
    14341 tgttatcgcc gctgatggcg ttaaaggtaa tggtcggcag cccggtattg accgtcacct
    14401 gatgggtagc gctgccgctg tttcctccgg catcggtgac gctggcattg atggtcaccg
    14461 tgccattcgc cagtgccgag acgacgcttg ccggaacgcc gacgctccag ttgccgctgg
    14521 catccagcac cgtggtaaag gtatttgtgc cgatagtcac cgtcacggtg ctaccggttg
    14581 ccgctccagt ggcggagccg ctgatgatct gcgcctgggc gtgttccgtc gcgttgatca
    14641 catcatcgcc tgccaccgtg ttgatggtga cgaccggaac ggacgtatcc accgtcaggt
    14701 tgtgattaac cgaggccggg tttccggctt tgtcgctcac cgccgcgttc acggtgtagt
    14761 tgctggcggt cagggtgctt aagtcggctg acggtacgct gacgctccag ctgccgtccg
    14821 tctgtacggt gccggtgtag tttgtgccat tgagcgtgac ggtcaccgtc tgccccgctt
    14881 ccgccgtgct gctgccggtg atggtcagag ccgctccggc ttcggcggcg ttaagaatat
    14941 catcgcccgc aatggtatta atggtcaccg ttggcgcgct ggcatcaacg ctgtaagcgt
    15001 gggtcgtggt ggcgctgttg ccgttaacat tgctgacgct ggcctgtgcg ctggcatcgc
    15061 catcacgcag agccgccata tctgccgccg gaaccgaggt gctccaggaa ccattcgcag
    15121 ccaccgtggc gctgtaagtt ttgccgccaa aggtgacggt aaccgtttgt ccggcttcaa
    15181 cgccagaggt gctgccggag agcgttaacg ccgcgccttt ttcggcagcg ttgatcacat
    15241 catcggcggt aatggcgttg atgctcaccg ccaccgccga gagatcgacc gtcaccggat
    15301 gcgtaacgct taccggattt ccggcagagg tgctaccgct cgccgcaatc gtcaccgatc
    15361 ccgcaggcca ggcgctgaca tccaccgccg gaacgccgac gctccaggtg ccatccgcca
    15421 gcaccgccgc aacataggtt tgcccgttaa tggtcagcgt gacgttgctg cccgccgcca
    15481 gaccggagct gctgccggta atcaccagcg cctgcccgtg ctcgataata ttaaccacat
    15541 catcgcccgc cacggtgtcg acccgcagac cggggagatt cgcgtctatg gtgatttcgc
    15601 gcgtgccgtt accagtattg cctacgctgt tcgtcaccga agccgaaatg gtcagttcgc
    15661 cgttgcccaa ctcctgtagc gcggaggccg gaacatcgac gctccagctt aaattcgcct
    15721 gcaccgtggc agtgtaagtc gccccgccga gcgtgacggt caccgtgtcg cctgccgccg
    15781 cacccgtcac ctgcccgctg atggtctgtt ccacgcccgc ttcggcggcg ttaataatat
    15841 cgtccgttgc caccacgtta atggtgacgc ccggcagcgc ggtattaacc tgtacgttat
    15901 ggctggcgga accgctgttg ccatcggcgt cagtggcggc tgcggtcacc gtgtaggtgg
    15961 cttcaccgag ggcgctcacc cgtgacgccg gaacggtcac gctccagtta ccggaagcat
    16021 cggcagtggt ggtgtaattt tgcccgttga gcgtaacggt aatctgcgtg cccgcaggct
    16081 ggttgctggt gccggtaatc gccagatccg cgcctttctc cgcggcgttg atgatgtcat
    16141 cgacggcaat ggtgttaatg gcgagcaccg gagcgccgag cgccacggtg acagtgtgcg
    16201 aggccgtgcc gctgttgcct gcggagtcgg tgaccgtagc ggtaatggtc acatcgccct
    16261 gcgccagcgc ggaaatcacg ctggcaggca cgccgatgct ccagttgccg ttggcgtcca
    16321 gcacggtggt ataggtggtc gtgccaatcg tcacggaaac cgtattaccg gtagtcgcgc
    16381 ccgttgccga gccgctgatg atctgcgcct gtccgtgttc cgtggcgtta atgatgtcat
    16441 cgcccgccac cgtgttgatg gtgacgaccg gagcggcaag gtccaccgtc agattatgcg
    16501 ttgccgaagc cgaatttctg gctttgtcgc tgaccgaggc gttaacggta tacgagctgg
    16561 cggtaaggct agctaaatcg ccggttggta cgctgacgct ccagctcccg tctgcctgca
    16621 cattgccgct gtaattaacg ccgtttagcg ttacggtcac cgtctgcccg gcttccgccg
    16681 tgctggtgcc gctgatggtc agcgccgatc ccgcttcggc ggcgttaaga atatcgtccg
    16741 tggcgatggt gttgatggta acggagggcg cagtggcatc aacgctgtaa gcatgcgttg
    16801 cctgggtgct gttccctgcg acattgctga cgctggcctg gacgtttgct gcgccgtctg
    16861 gcaaggttgc aacatcgacg gcagggatcg tcagccccca ggtattatcc gcggcaaccg
    16921 tggtggtgta gcttttgccg ccgaagataa cggtgatggt ttgccccgct tccacgccgg
    16981 aggtggtgcc ggagagctgc aaatcagagc ctttctcggc ggcgttaatc acatcgtcag
    17041 tggaaagggt gttgatggtg atcgccacgg cagcgagatc gaccgtgacc ggatggctaa
    17101 tgctggttgt ggttccggcg gtgttggtgc cagagacggc aatattgacc gtccccgcag
    17161 gccagtttgt gacatctgcc accggaacac caacgctcca gctaccatcc gctaaaaccg
    17221 ttgcgccgta ggtgacgcta ttgataacca ccgtcaacgc cgcgcccgcc gccagtccgc
    17281 tactgctacc ggtgatcacc aacgcctgag tgtgctcgat gctgttaatc acatcatcgc
    17341 ctgccacggt atcgacgcgc agacctggca ggttggcatc tatcacgata tcgtgcgtcg
    17401 cggtgccggt attatttgcg gaattggtca acgaggcgct gatggtcagt tcaccgttac
    17461 ccagcgcctg caaaacgttc gccggtacgc tgacgctcca gcttaaatct gactgcacgg
    17521 tggcgttata ttgattgccg ccaatggtga caatcaccgt attcccggca accgccgttc
    17581 ccgtgacctg accgctgatg gtctgcgcca caacgatttc agcggcgttg ataatgtcat
    17641 cgccggagac ggtgttaatg gtcagcgtcg ggccgctaag atcaacggtc acatcgtgag
    17701 tcgtgctgtc gctgttgccc gcgcgatcgc tcaggctggc ggtgatggtc tggctaccgg
    17761 tcgccagcgc cgtgacatcc gccgccggaa tgcccacgct ccagttaccg gaggcatcca
    17821 gcgtggtggt gtagttttta ccgttcagcg tgacggttac cacatcaccc gccgccgcgc
    17881 ccgtactggt gccgctgacc accaacgcct gatcatgttc gatggcgttg ataatgtcat
    17941 cgcccgcgac ggtgttaatg gtgagatccg gcgcagtgat atctaccacc agcgcgtggt
    18001 cggcgcttgc cgggttaccc gctttgtcgc tcaccgtggc ggtcacggtg taactgctgg
    18061 cggttaatcc gcttaaatcc gctgccggaa cattcacgct ccaggtgcca tccgccagaa
    18121 cggtggtctg gtacgtgttg ttattgagcg tgacggtgag cgtctgcccg gcctgcgccg
    18181 tggtagtgcc gctgatggtg atccccgcgc cagcttcgct cacgttaagg atatcgtcgc
    18241 tggcgatggt gttgatggtc acaagcggcg cggtggcatc aacgctatac gcgcgatcgg
    18301 cctgggcgct gttgccgtta atattactga cactcgcctg cacgttgccc gcgccgtccg
    18361 gtagcgcagc gagatcggca ggcggcacat tcaccgtcca gctaccgtta gcttccactg
    18421 tggtggtgta atttttgccg ccaaaggtga cggtcaccgt ctgcccggct tcgacaccgc
    18481 tggtgctgcc gcttacgacc agcgtttcgc ctttttcgac ggcgttgatc acatcatcac
    18541 cagagagagt gttaatggtg atggcgaccc cagccagatc gacggtaacc ggatgggtaa
    18601 tggtggaggt tgttccggca ctgttggtgc cgcttaccgt gatattcacc gtacccgctg
    18661 gccagttgct gacgtctacc gccggaacac cgaggctcca cgtgccgtcg gctaataccg
    18721 tcgcgccata agtaacgttg ttaatctcga ccgttaacgc cgttcccgcc gttaatccgc
    18781 tgctgctgcc ggtgatcacc agcgcctgat tgtgctcaat gctgttaata acatcatcgc
    18841 ccgccacggt atcgacccgc aggccaggca gattggcgtc gatcgtgata tcgcgcgagc
    18901 cgctgccggt gttgccgacg ccattggtga ccgaggcatt aaccgtcagg tcgccgttgc
    18961 ccagcgcctg aatatccgcc gccggaacgt ccacactcca gcttaaatta gactgcaccg
    19021 tggcggtgta ggtgttgccg cccagcgtaa cggtaaccgt atccccctgc gctgcgcccg
    19081 tcacctgccc gctgatggtt tgcgcatttc ccgattcggc agcgttaata atatcgtcag
    19141 tcgccaccgc gttaatggta acggcaggca aggcgctgtt gaccagcaca ttatgactgg
    19201 cggaattact gttgcctgcc gtatcagtga cgtttgccgt taccgtgtag ttggcttcac
    19261 ccaatgcgct aaccgctgac gctggcaccg tggcgctcca gttgccgttg ctatcggtag
    19321 tagcggtgta attttgcccg ttcagcgtca ccgtaatggt ggtgcccgca ggctgattac
    19381 tggtgccggt gatttgcagg tccgcgcctt tttccgtggc tttaatcacg tcatcggtgg
    19441 cgatggtgtt gataccaatg gttggcgcgg cgagattcac ggtgaccgtg cggctggcgt
    19501 catcgctgtt gcctgccgcg tcggtaattg ccgcagtgat agtttgcggg ccgctgccaa
    19561 gcgcagtgac gtcagccgcc ggaacgccga cgctccagtt gccggaagcg tccagcatgg
    19621 tggtgtaggt tttactgttt agtgtgacgg tgatgacatc acccgcttcg ccgccagtgc
    19681 tggagccgga gatcaccagc gcctgtccgt gttcggcggc gttaataatg tcatcgccgg
    19741 agacggtgtt gatggtcagc accggcacgg tgagatccac cgccagcccg tggttagcgg
    19801 acgccgggtt acccgcttta tcgcttaccg aggcactaac ggtgtactgg ctggcggtca
    19861 gattgctgag atccgccgtc ggtaagctga cgctccagct gccgtccgcc tggacggagc
    19921 cgctgtaagt cacaccatta agcgtgacgg ttaccgtctg ccccgcttcg gcggtgctgc
    19981 taccgctgat ggttaacgga ttgcccgcct cggcagcgtt cagaatatcg tcggtggcga
    20041 tggtgttaat ggcaagcgtc ggggccgtgg catcgacgct gtaggcgtgg gttgccgaag
    20101 ccgtgttgcc gttaatagtg ctgacgctgg cctgcacggt ggcgtcgccg tcgcgtaaca
    20161 cgctgagatc ggcggcgggt acggtggttg tccagctacc atcgcccgct acggtagcgg
    20221 tgtaggtttt cccgccaaag gtaacggtga ccgtttgccc cacttccacg ccggaggtgc
    20281 tgccggaaag ggttaaatct gccccttttt cggcggcgtt aatcacatcg tcgccggaaa
    20341 cggtgttaat ggaaatcgcc accgccgcca gatcgacggt gaccggatgg gtgatggtgg
    20401 atgttgttcc ggctgtgtta gtgccactta ccgtgatatt caccgtaccc gcaggccagt
    20461 tactcacgtc tgccgccgga acaccaacgc tccatgtgcc atcggctaat actgttgcag
    20521 cgtaagtgac cgtgttaatc accaccgtca gcgccgcgcc cgccgccagc ccgctgctgc
    20581 taccagttat caccagcgcc tgagcgtgct cgatgctatt gaccacatca tcgcccgcca
    20641 cggtgtcaac gcgcagacct ggcaggttgg cgtcgatagt aatatctcgc gaaccgctgc
    20701 cagtattgcc aacgccgttg gtcaccgaag cgttaaccgt cagattgcca ttgccgatgg
    20761 cctgaatatc cgccgccgga acgtccacgc tccagcttaa attcccctgc acggtagcgg
    20821 tgtaagtttt cccgccaagc gttacggtaa ccgtatcgcc cgccgccgcc cccgtcacct
    20881 ggccgctgat ggtttgcgca ttgcccgatt cggcggcgtt aataatatcg tcggtcgcaa
    20941 ctgggttaat ggtgacgcca ggcagcgcgg tattgacctg cacgttatgg ctggcggaat
    21001 tgctgttgcc tgcgctgtcg gtgacgttcg ccgtcaccgt gtagctggct tcgcccagcg
    21061 cacctactgc ggaagctggc accgtagtgc tccagttgcc tgcagcgtcc gtcgtcgcgg
    21121 tgtaattttg cccgttaagc gccagcgtaa tcgtggtgcc tgctggctga ttactggtgc
    21181 cggaaatttg cagatccgcg cctttttcgc tggcgttaat tatgtcatcg gtggcgatgg
    21241 tgttgatgtc aatggttggt gcggtgaggt taacggtgac cgtgtgtgtc taattgtcgc
    21301 tgttgcctgc cgcatcggta accgtggcgg tgacagtttg cgggccgctg ccaagcgccg
    21361 tgacatccgc cgccggaacg ccgacgctcc agttgccgga ggcgtccagg gtggtggtgt
    21421 aggttttact gtttagcgtg acggtgacga catcccccgc ttctccgccg gtgctggaac
    21481 cggaaatcac cagcgcctgc ccgtgttcgg tggcgttaat aatgtcatcg cccgcaatgg
    21541 tgttgatggt cagcaccgga acggtgagat ccaccgccag attatgcgtg gtgctcgccg
    21601 gattgcccgc tttgtcattg accgtggcgg tcagggtgtg gctgccatcg gtcaatgcct
    21661 caagatctgc cgccggaata ttgatgctcc agctgccgtc cgcctgcacc gttgcctgat
    21721 aggtctgcac ggtcgggctg ttaagcgtta tcgtaacaat ctgccccgct tcggcggtgg
    21781 tactgccgct caccgtcacg cccgcatcgg cttcgctggc gttgacgata ttgtcgctgg
    21841 cgacggtatt gataatgatg gttggcgcgc tgctgtcgac gctgtagttg tgcaccgccg
    21901 aggcgctatt gccgttgata ttgctgacgc tggccagtgc ggaggcgctg ccctcgggta
    21961 atgacgccag atcggcggcg ggtacggtgg cagtccagct accatcgctc gctacgctgg
    22021 cagtgtaatt tttcccgcca aaggtgacgg tgacggtttg accgggttct acgttagtgg
    22081 tggtgccgga aagggttaaa tcagcgcctt tttctgcggc gttaatcaca tcgtccgtgg
    22141 cgatggtgtt gatggtgatc gctgccggag tgagatccac cgtcaccgga tgcgtaatgc
    22201 tcaccgagtt tccggcgcta ctttcccctg aaacggcaat attaaccgtc cccgcaggcc
    22261 aggcgctaac ctgcgccgcc gtgacgccca cgctccagct accatcggcc tgcaccgcag
    22321 tggtgtattc cacattatta atcgtaacgg taagcggcgt gctttcagcc agccccgagc
    22381 tgctgccggt gaccaccagc gcctgcccgt gctcgatgat attgaccaca tcatcgcccg
    22441 ccaccgtatc gacccgcagg ccgggcagat tggcgtcgat ggtgatatcc cgcgtgccgc
    22501 tgccggtgtt gccgttttga ttggtgactg aggcattaac cgttaaatcg ccatttccca
    22561 gcgcctgaat atctgccgct ggaacgtcca cgctccaggt gagattgctg cccaccgtcg
    22621 ccgtataagt attaccaccc aacgtaatag ttaccgtgtc gccatcttcc gcgccagtca
    22681 cctgcccgct gatggtttgc gccacgcccg cttcggcggc gttaataata tcgtcggttg
    22741 ccaccggatt aatggtcaca ccgggcagcg cgctgtcgac cagcacgtta tggctggcag
    22801 tggcgctgtt gccgatatcg ctggtcaccg ccgccgttac cgtatagttg gcctggccta
    22861 atgctgtaac cgccgatgcc ggaacggtga cgctccagtt gccggaggcg tcggtcgtgg
    22921 tggtgtaatt ttgcccgttc agcgtcacgg tgatggcggt gttaacaggc tgatcgctgg
    22981 tgccggtaat ctggagatcg gccccttttt cgctggcgtt aatcacatca tcgcccgcaa
    23041 tgctgttaat gccgataagg ggcgcggcgg tattgaccgt gacggtcaat gactggctgc
    23101 ccgtgtttcc ggctttgtcg gttaccgaga cgctgacagg ataactgccg tccgccagcc
    23161 cactgaccac cgaggccgga acgcccagac tccagttgcc ggaaccatcc accaccgtgg
    23221 tgtaatccac attattcagc gtcacggtga cgatatcgcc cgcctgagcg ccagtaaccg
    23281 tgccgctgat gatttgcgcc tgagcatgtt ccggcgtgtt gatgacgtta tcggtcgcca
    23341 ccggattaat ggttaccacc ggcggcgtgg catccaccag cgccacgcgt gaaacgctgc
    23401 tcggattacc ggcgacatcg ttcacgctgg cggtgaccgt agcgttgccg tcagccagtg
    23461 cgcccacgtt ggcggcgggc acagtcacgc tccaggtgcc gtctggctgc acgacgccct
    23521 gatacgtctg cccatttaac gtaacggtca ccgtctgccc cggctgagcg tcggtggtgc
    23581 cggtgatggg cagatcctgc ccgctttcgg cggcgttaat gatgttatca gcggtcagtg
    23641 gatcaatgct taaggccggg gcctggctat cgacggtaat ggtgcgggaa gtgttgccgg
    23701 tattgccgct gagatccgtc acagaagcgg taatcgccac cgcgccgtca ggcagatttc
    23761 ccatcgcggc ggcaggtacc gtcagactcc aggagccatc ggcctgcacg gtagtggtaa
    23821 aactctgtcc agccagggtt actgtgacgg tttgtcctgt ctcagcctgt tgagtgctac
    23881 cggtaagggt cagtggcgca cctttttctg cgccactgat aatgtcatcg ccggagacgg
    23941 tgttaatggt gatcaacgga gaaacaccga ccagcgtgat tgctcgagac gccgcgccag
    24001 tatttcctgc agcatctgtc acggtgacga aaatcgtatt ggctccgcga tccagcgtgc
    24061 gggttaccgc tgggtccagc gccacattcc agctgccatc tgccagcacg atgcccgtca
    24121 ggacatgagt gcccaatttc accgttaccg tatcgcctgg agaagcaccg ctgacttgtc
    24181 cggagatgat ctgcgcgacg gcctgctcgg cattattaag aatatcgtcg cccgccacgg
    24241 tattaacact gaccacgggt gctgaggtgt ctaccgtgaa attcgcgctg tggctggtgg
    24301 tgttccctgc ccgatcgctg acactggcat tgacagcgta attaccctcc gccagtgcct
    24361 ggacttccgt ggcaggcagc gtcacttgcc aggttccgtc gctaccgacg gttgctgtat
    24421 ggcttttccc gttgacggtc agtgttacgg tttgccccgc ttctgcattc gacgtgccgc
    24481 tcagtaccag cgcgacgtta tgttccgcag cactgataat gttgtcctga gcgatggtgt
    24541 tgatggcaag cgttggcggt tgggtatcga ccgttagtag ctgcgcgccg gtgaccgtat
    24601 tgcctgtggc gtctttccca ctgaccagca ccgcctgatt cccttcgcct aacgcctgtg
    24661 catcggcggt cggcacctgc acgctccagg caccgttagc gcctacgctg gtcgtgtagg
    24721 ttttgccgtt tagcgtaacg gtgagttctg ttccctgcgc cagatggcta ctggtgccag
    24781 aaagcgtaaa cccggcggcc tgttcgctgg cgttaataat gttatcgcct gcggtggtgg
    24841 caacctgcac gctggcctga ctattgtcga tggttaacgt tatcgaaccg gcgtcagtga
    24901 tggtactgcc gacaatttgc accactgacc aggtatgttc acctgcactt tgcgtgggta
    24961 acgagacact ccagtgaccg ttgttgtcta cctgagtact ggcaatagta ttgccgttac
    25021 tgtctttaat ctgaatagtc gcccccgcct gcccgctgcc gctaaaggtt ggcgtggtgt
    25081 catcggtgat gtcattggtg gctagaatgc cgcgctgatc gccctgatta tcggtaacta
    25141 aaaatgatgg tgtggcgttg ccaggctcag caggtggcgt agggttatta atcacttctg
    25201 ttttcgagtc gccatcgcca ccgcttgcca gcagtgcacc aagcgcgcca ccacctaccg
    25261 ccgcccccgc cagccaaccc cacgggaagg cgctggtctg agcaacggta tcaagaaatg
    25321 gcgcaatgct ttcaatcgca gtggtctggg cagtcagttc tacgggggct aatccacccg
    25381 cagcagtatc ggcaaaggtg atatgggtta gctgttgacc atcggcaaac accagttccg
    25441 attgttcagc tgtattcgcc gcttgcagga aataaccgtt gcagcggatc accgtgccgt
    25501 cccgcatata gatcagcagg tcatttcctt cacggacgta acgcgcaacg gcagaggcct
    25561 gagcatgaac ctgcaccacg gaaggctcat aaataacaac cgtgacgtcg ccaggattaa
    25621 tcagcgtttg tttgacggat gttttacgcg aaataacatc aataatcagg ctcataatgg
    25681 cattcctgat aaatggcgca catccttagc gagtccattt atctaaataa gacaaaggta
    25741 atcacttaac aacggcccac tattaataat ggcaggatta tttcaagtcg ggtaagtcgt
    25801 tgtataagcc ctgtttaatt cccgctaatg gaataaggtt attcaccgca gcggcataat
    25861 ttacggcggc gacccagcca tcataattgg catttatttc agccgattgc gcctgaaaga
    25921 catcttgttc gacggttaat agatcattca gactgcgttt acccaattta tattcatttt
    25981 ggtacacatc tcgcgttttg tgcgcacttt cggattgcgc taaaccggct tcttcacgac
    26041 cccgtgcgcc ggtccagttc gcatatgcca cagacgctcg ttgcagcaca tccagttttg
    26101 cctgttcgac ctgcgaggca gagatttttt gttgcccttc ggcctgttgc acctgggcag
    26161 aaaccgcgcc gccctgatac agcggcgcgt taacgttcag ttgtagctga tcatcccaat
    26221 acgagcggtc gctggtctgg tagcgcgttt tacccccctg aatactgagc gttggccagt
    26281 attgcgcttt cgttttttcc acgccgtact gtgctgactg gcgtaagttt tctgccgcca
    26341 gcaccagcgg gatggactgg taatcaatat tcttcagcga taccggctgc tcggctaatt
    26401 cagcaggtgg cgcggctatc gcctccggct gtacgccagt gagcaccgcc agttgcgctt
    26461 tggcgctcgc catctgcgcc tgatattgct ccagcgttga gcgcattccg gcaatacgcg
    26521 tctgggcctg taattcatcc gacgatgagt tcaggcctgc gttagcgcgc aatgccgcca
    26581 tgttgtagac gttttccagc gagtgaatat tgcgttgagc cgcatcgcac aaagcctgat
    26641 aacgactaac ttccatgtag gcagtcgccg ttttctctgc aacatcggtt aaggttgcca
    26701 tcaatttaaa gcggtagctg tcacgggcgg cagtttgcag attgatatcg ttgttagttt
    26761 taccaaagtc atacaccagt tgtgtcaggg ttatgccata cgaaacgttg ttatcaagcc
    26821 tgccgctgga gtccgtttgt cgggacggcc ccgcgttgcc ggttaatcca acgtgcggat
    26881 accaggcact tttggcttca tcaatttgcg cttcgccaat acctatctgc gccgcctgtt
    26941 gagtgacacc gggatttcgc gcaaaggcgc gtaatatact ttcctgtaaa gataattggg
    27001 caactggcgt taccgctggg gcattcgccc attgtggcag cggtgcagca attgcagctt
    27061 ctcccatcac cataaaactt ataaaaaaaa taagtccatt atttttacgg tttttaaact
    27121 ggaatcgcgc catagataag tcacttcctt c
//
LOCUS       DBFIFM010000044        27035 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.044, whole genome shotgun sequence.
ACCESSION   DBFIFM010000044 DBFIFM010000000
VERSION     DBFIFM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.044"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(126..1790)
                     /gene="glnS"
                     /locus_tag="N7C74_001639"
     CDS             complement(126..1790)
                     /gene="glnS"
                     /locus_tag="N7C74_001639"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HGU2431531.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRSD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1993..3939)
                     /gene="nagE"
                     /locus_tag="N7C74_001640"
     CDS             complement(1993..3939)
                     /gene="nagE"
                     /locus_tag="N7C74_001640"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HGU2431532.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4272..5072
                     /gene="nagB"
                     /locus_tag="N7C74_001641"
     CDS             4272..5072
                     /gene="nagB"
                     /locus_tag="N7C74_001641"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGU2431533.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5132..6280
                     /gene="nagA"
                     /locus_tag="N7C74_001642"
     CDS             5132..6280
                     /gene="nagA"
                     /locus_tag="N7C74_001642"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGU2431534.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6289..7509
                     /gene="nagC"
                     /locus_tag="N7C74_001643"
                     /gene_synonym="nagR"
     CDS             6289..7509
                     /gene="nagC"
                     /locus_tag="N7C74_001643"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HGU2431535.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7557..8309
                     /gene="nagD"
                     /locus_tag="N7C74_001644"
     CDS             7557..8309
                     /gene="nagD"
                     /locus_tag="N7C74_001644"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HGU2431536.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8566..10230
                     /gene="asnB"
                     /locus_tag="N7C74_001645"
     CDS             8566..10230
                     /gene="asnB"
                     /locus_tag="N7C74_001645"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HGU2431537.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDKA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10610..10686
                     /locus_tag="N7C74_001646"
     tRNA            10610..10686
                     /locus_tag="N7C74_001646"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10644..10646,aa:Met,seq:cat)
     gene            10697..10781
                     /locus_tag="N7C74_001647"
     tRNA            10697..10781
                     /locus_tag="N7C74_001647"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10731..10733,aa:Leu,seq:tag)
     gene            10805..10879
                     /locus_tag="N7C74_001648"
     tRNA            10805..10879
                     /locus_tag="N7C74_001648"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10837..10839,aa:Gln,seq:ttg)
     gene            10914..10988
                     /locus_tag="N7C74_001649"
     tRNA            10914..10988
                     /locus_tag="N7C74_001649"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10946..10948,aa:Gln,seq:ttg)
     gene            11004..11080
                     /locus_tag="N7C74_001650"
     tRNA            11004..11080
                     /locus_tag="N7C74_001650"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11038..11040,aa:Met,seq:cat)
     gene            11129..11203
                     /locus_tag="N7C74_001651"
     tRNA            11129..11203
                     /locus_tag="N7C74_001651"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11161..11163,aa:Gln,seq:ctg)
     gene            11241..11315
                     /locus_tag="N7C74_001652"
     tRNA            11241..11315
                     /locus_tag="N7C74_001652"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11273..11275,aa:Gln,seq:ctg)
     gene            complement(11469..12644)
                     /gene="ubiF"
                     /locus_tag="N7C74_001653"
     CDS             complement(11469..12644)
                     /gene="ubiF"
                     /locus_tag="N7C74_001653"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HGU2431538.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQVEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12790..14214
                     /gene="miaB"
                     /locus_tag="N7C74_001654"
     CDS             12790..14214
                     /gene="miaB"
                     /locus_tag="N7C74_001654"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HGU2431539.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14367..15407
                     /locus_tag="N7C74_001655"
     CDS             14367..15407
                     /locus_tag="N7C74_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HGU2431540.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15404..15871
                     /gene="ybeY"
                     /locus_tag="N7C74_001656"
     CDS             15404..15871
                     /gene="ybeY"
                     /locus_tag="N7C74_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HGU2431541.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQMVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            15961..16839
                     /gene="corC"
                     /locus_tag="N7C74_001657"
     CDS             15961..16839
                     /gene="corC"
                     /locus_tag="N7C74_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HGU2431542.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16864..18402
                     /gene="lnt"
                     /locus_tag="N7C74_001658"
     CDS             16864..18402
                     /gene="lnt"
                     /locus_tag="N7C74_001658"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HGU2431543.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIRWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            18799..19707
                     /gene="gltI"
                     /locus_tag="N7C74_001659"
     CDS             18799..19707
                     /gene="gltI"
                     /locus_tag="N7C74_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HGU2431544.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            19877..20617
                     /gene="gltJ"
                     /locus_tag="N7C74_001660"
     CDS             19877..20617
                     /gene="gltJ"
                     /locus_tag="N7C74_001660"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HGU2431545.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQASKLLDYSAHAWESFTAITLA
                     YVFINAFIMLVMTLVERKVRLPGNMGGK"
     gene            20617..21291
                     /gene="gltK"
                     /locus_tag="N7C74_001661"
     CDS             20617..21291
                     /gene="gltK"
                     /locus_tag="N7C74_001661"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HGU2431546.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            21291..22016
                     /gene="gltL"
                     /locus_tag="N7C74_001662"
     CDS             21291..22016
                     /gene="gltL"
                     /locus_tag="N7C74_001662"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HGU2431547.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            22134..23069
                     /gene="rihA"
                     /locus_tag="N7C74_001663"
     CDS             22134..23069
                     /gene="rihA"
                     /locus_tag="N7C74_001663"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HGU2431548.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            23153..24823
                     /gene="hscC"
                     /locus_tag="N7C74_001664"
     CDS             23153..24823
                     /gene="hscC"
                     /locus_tag="N7C74_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HGU2431549.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPIHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKRTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRQASVYLAIEIP"
     gene            complement(24883..26334)
                     /gene="djlC"
                     /locus_tag="N7C74_001665"
                     /pseudo
     CDS             complement(24883..26334)
                     /gene="djlC"
                     /locus_tag="N7C74_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
     gene            complement(26331..>27035)
                     /locus_tag="N7C74_001666"
     CDS             complement(26331..>27035)
                     /locus_tag="N7C74_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU2431550.1"
                     /translation="NKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSSW
                     GINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFVA
                     QTAMCCGEGGIKAWDYVRMGFLSRVGVLNKWLTEDESLWLQSRVYERAHHYYHSWMHY
                     FSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQPL
                     TVQPECPVTLKDVSDL"
BASE COUNT         6629 a         7021 c         6874 g         6511 t
ORIGIN      
        1 tatattgcag agatcatgta ggcctgataa gcgtagcgca tcaggcaatt tagcgtttga
       61 aactgtttgt ctgttccttg ccggatgcga cgtaaacgcc ccatccggca tagcgaaact
      121 taaaattact cgcctacttt cgcccaggta tcacgcagcc caacggtgcg gttaaatacc
      181 ggtttttccg ccgtagaatg gcggctatcg aggcagaagt aaccttcacg ctcaaactgg
      241 aatgctttac ccgcaaccgc atctttcagc gacggttcag caaagccctg tttgatcacc
      301 agcgattccg ggttaatcac cgacaggaaa tcatccgcag cacctgggtt cggcacgctg
      361 aacagacggt catacaaacg gatttcaacc ggcagcgcat gtgccgcgct cacccagtga
      421 ataacacctt tgactttacg accatctgcc ggatctttgc ttaaggtatc ggcgtcataa
      481 gtacagaaga tggtggtgat attaccttcg gcatctttct cgacgcgttc agccttaatc
      541 acataagcat tacgcagacg cacttcttta cccagcacca gacgtttgta ctgcttgtta
      601 gcttcttcgc ggaaatcgga gcgatcaatc caaatctcac cgctaaacgg cacctgacgg
      661 ctccccattt ccggtttgtt cggatggttc ggcatggtaa ccatttcgcc ttcgccctga
      721 tagttttcga taaccagttt caccggatcg ataaccgcca ttgcgcgcgg cgcattttcg
      781 ttgagatctt cacggatgca ggattccagc gacgccatct caatggtgtt gtcctgcttg
      841 gtcacgccga tgcgtttgca gaactcacga atagaagccg cagtgtaacc acgacgacgc
      901 agaccggaaa tggtcggcat acgcgggtca tcccagcctt caacgtgctt gtcggtcacc
      961 agcaagttca gcttacgctt ggacatcaca gtgtattcca gattcaggcg cgagaattcg
     1021 tactggcgcg ggtgaacagg aatggtgatg ttgtccagta cccagtcgta cagacgacgg
     1081 ttatcctgga actcaagcgt acacagagag tgcgtaatac cttccagggc atcgctgatg
     1141 cagtgggtga agtcgtacat cgggtagatg caccacttgt tgccagtctg gtggtgttca
     1201 gcaaatttaa tgcggtacag caccggatcg cgcatcacga taaacggtga agccatgtcg
     1261 attttcgcac gcaggcaggc tttaccttct tcaaaaccgc cggtacgcat tttctcgaac
     1321 agcgccaggt tctcttcaac gctacggtcg cggtacgggc tgtttttacc aggctgcgtc
     1381 agggtgccgc ggtattcgcg gatctgttcc ggcgtcagtt catcaacgta cgccaggcct
     1441 ttattgatca gttcgatcgc ataggcgtgg agctgatcaa aataatcgga ggagtaacgg
     1501 acgttaccag accagtgaaa acctaaccac tcaacgtcgt ttttgatcga atcgacatac
     1561 tcgatatctt cttttaccgg gttagtgtcg tcgaaacgca ggttgcactg gcctttatag
     1621 tcctgggcga tcccgaagtt caggcagata gatttcgcat ggccaatatg cagatagcca
     1681 ttcggctccg gcgggaaacg ggtgtgtact gtggtgtgct taccactggc cagatcttca
     1741 tcgatgatct gacggataaa gttagtcggg cgggcttctg cctcactcat cgtggattcc
     1801 tcaaagcgta aacaacgtat aacggcgtat gatcttataa gcgggacagg ctgacaactg
     1861 ttagttagtg aaaatcgaaa atctcactga gattatgggg atgattgatg aaaaaaaaag
     1921 cggcagagaa gatctccacc gcttgtcatt gttggatgcg acgctcaagc gtcgcatcag
     1981 gcataaagca gattactttt tgatttcata cagcggtgtt tgacccgcca caacatggcc
     2041 ctgagcttta atgatcaagc cactgaaatc gtcgatattg ctgcaaacca ccgggctaat
     2101 catcgaacgg gcgttagcgt tcaggtaatc cagatccatt tccagaatcg gttgccctgc
     2161 gcttacctgc gcgccctctt ccaccagacg tttaaagcct ttaccttcca gcgctacggt
     2221 atcgataccc atgtgaacga caatctccgc gcctttttcg gtttccaggc agaacgcgtg
     2281 gttggtgttg aagattttca cgattgttcc tgcggctggt gatacgacga ttttatctgt
     2341 cggtttcacc gccacaccgt cacccaccgc tttgctggcg aatgcttcgt caggaacctg
     2401 atccagtgcc acgacatcac cggtaatcgg cgataccagc tccgcgatag ataccgcgtt
     2461 tggtacagcc tgcggttttg ctacaggagc ggcagttgcc ggagttgctt cagcagaggc
     2521 agctgctacc gggccacggg caacgacttt cttcatcgca tcgccgatgg attctgcttt
     2581 cgcgccaaca atcacctgaa tagtctgttt gttcagtttt actaccccag aagcccccag
     2641 acgtttacac atcgtatcgt taacgcgggc agagtcagcc acggtaaggc gcagacgggt
     2701 gatacaggcg tcaattgctt tcaggttgtc agtgccgcca accgcagcaa tatagttggt
     2761 cgccagttga ttgaggcctt cttcagtgtt gctgttggct tcttcagtaa cgatctcgtc
     2821 ttctttatct tcacgacccg gcgttttcag gttgaacatg cggataacca aactgaacac
     2881 cacgaagtag atagcgaaga agacaacacc cataaccagc agcatccaga cgttctggct
     2941 ggcggccggc aggttataca tcaacgcgta gtcgatagcc cccgcagaga aagagaagcc
     3001 ggcatggata cccagcagcg ttgccacaaa caggctgata ccggtcagca gtgcgtgcag
     3061 gaggtacagc agcggagcaa ggaacatgaa caggaattcc agcggctcag tcacaccggt
     3121 caggaacgca gtaacagcaa cagaaagcag catcccgcca accatcggac gacgctcttt
     3181 cggtgctgcg aagtacatcg ccagcgccgc acccggcagg ccgaacatca tgatcgggaa
     3241 gaagccggac atgaacatcc ccgcggtgcc gtcaccagcg tagaaacggt tgatgtcgcc
     3301 gtggaaaacc gtacccgccg cgttggtgaa ttcaccaatc tggaaccagg cgatggtgtt
     3361 cagcacctga tgcagaccgg ttgggatcaa cagacggttg atgaaaccaa agataccgga
     3421 acccagcgcg cccgcagaaa cgatccactc gccgcctgca tggatagcgt gctgtaccgg
     3481 cggccagacg taaccaaaaa tggccgccag caccagacag aagaagccgg tggcgatcgg
     3541 cacaaagcgt ttgccgccga agaagctcag gaagtccggc agtttaatat cggaccaacg
     3601 gttataggct gcgccaccaa ccagaccggt aatgataccc gccagtacac ccatgttaat
     3661 ttctgggttg atggtcacca tcgctttggt taacacaaag taacctaccg cccccgccag
     3721 cgccgccgca cctgcgctgt ctttcgacca gctggatgcc acaccgatgg cgaagattaa
     3781 tgcgaggtta tcaaaaatcg caccgcccgc ctgggcaata aacgcaacgt taagtaaatc
     3841 tggctgaccg aatcgcagca acagtgccgc caccggcagc accgcgatag ggagctgtaa
     3901 cgccctaccg agtcgctgga aaaaacctaa aatattcatc ttattccccc tacgagaacc
     3961 ctatttggct cgtttaaagc cgtattttta ttgtgctgca aattgtactg ccgatgttct
     4021 gtaatcagat tgttagatca tctgccacag agtgtgtgaa aatttaattc gtatcgcaaa
     4081 ttaaacgagt gtcttttgtg agttttgtca ccaaatatcg ttatcatcac tcccttttac
     4141 tggctaaacc agaaaactta ttttatcatt caaaaaatca ggtcggattg acgcctgtct
     4201 gcgcaaatcc aggttacgct taaagatgcc taatccgcca acggcttaca ttttacttat
     4261 tgaggtgaat aatgagactg atccccctga ctaccgctga acaggtcggc aaatgggctg
     4321 ctcgccatat cgtcaatcgt atcaatgcgt tcaaaccgac tgctgatcgt ccgtttgttc
     4381 tgggcctgcc aactggcggc acgccaatga ccacctataa agcgttagtc gaaatgcata
     4441 aagcaggcca ggtcagcttt aagcacgttg tcaccttcaa catggacgaa tatgtcggtc
     4501 tgccgaaaga gcatccggaa agctactaca gctttatgca ccgtaatttc ttcgatcacg
     4561 ttgatattcc agcagaaaac atcaaccttc tcaacggcaa cgccccggat atcgacgccg
     4621 agtgccgcca gtatgaagaa aaaatccgtt cttacggaaa aattcatctg tttatgggcg
     4681 gtgtaggtaa cgacggtcat attgcattta acgaaccggc gtcttctctg gcttctcgta
     4741 ctcgtatcaa aaccctgact catgacactc gcgtcgcgaa ctctcgtttc tttgataacg
     4801 atgtcaatca ggtgccaaaa tatgccctga ctgtcggtgt tggtacactg ctggatgccg
     4861 aagaagtgat gattctggtg ctgggtagcc agaaagcact ggcgctgcag gccgcagttg
     4921 aaggttgcgt gaaccatatg tggaccatca gctgtctgca actgcatccg aaagccatca
     4981 tggtgtgcga tgagccttcc accatggagc tgaaagttaa gactttaaga tatttcaatg
     5041 aattagaagc agaaaatatc aaaggtctgt aattgttatc cctgccctgg ctccttgctc
     5101 agggcaatat tttttaaaat cgggggtcag aatgtatgca ttaacccagg gccggatctt
     5161 taccggccac gaatttcttg atgaccacgc ggttgttatc gctgatggcc tgattaaaag
     5221 cgtctgtccg gtagcggaac tgccgccaga gatcgaacaa cgttcactga acggggccat
     5281 tctctccccc ggttttatcg atgtgcagtt aaacggctgc ggcggcgtac agtttaacga
     5341 caccgctgaa gcggtcagcg tggaaacgct ggaaatcatg cagaaagcca atgagaaatc
     5401 aggctgtact aactatctgc cgacgcttat caccaccagc gatgagctga tgaaacaggg
     5461 cgtgcgcgtt atgcgcgagt acctggcaaa acatccgaat caggcgttag gtctgcatct
     5521 ggaaggcccg tggctgaatc tggtgaaaaa aggtacccat aatccgaatt ttgtgcgtaa
     5581 gcctgatgcc gcgctggtcg atttcctgtg tgaaaacgcc gacgtcatta ccaaagtgac
     5641 cctggcaccg gaaatggttc ctgcggaagt catcagcaaa ctggcaaatg ccgggattgt
     5701 ggtttctgcc ggtcactcca acgcgacgtt gaaagaagcg aaagccggtt tccgcgcggg
     5761 gattaccttt gccacccatc tgtacaacgc gatgccgtat attaccggtc gtgaaccggg
     5821 cctggcgggc gcgatcctcg atgaagctga catttattgc ggtattatcg ctgatggcct
     5881 gcatgttgat tacgccaaca ttcgtaatgc taaacgcctt aaaggcgaca aactgtgtct
     5941 ggttaccgac gccaccgcgc cagcaggtgc caacattgaa cagttcattt ttgcgggtaa
     6001 aacaatatac taccgtaacg gactttgtgt ggatgagaac ggtacgttaa gcggttcatc
     6061 cttaaccatg attgaaggcg tgcgtaatct ggtcgaacat tgcggtatcg cactggatga
     6121 agtgctgcgt atggcgacgc tctatccggc gcgtgcgatt ggcgttgaga aacgtctcgg
     6181 cacactcgcc gcaggtaaag tagccaacct gaccgcattc acacctgatt ttaaaatcac
     6241 caagaccatc gttaacggta acgaggtcgt aactcaataa gagaaagtat gacaccaggc
     6301 ggacaagctc agataggtaa tgttgatctc gtaaaacagc ttaacagcgc ggcggtttat
     6361 cgcctgattg accagtacgg gccaatctcg cggattcaga ttgccgagca aagccagctt
     6421 gcccccgcca gcgtaaccaa aattacgcgt cagcttatcg aacgcgggct gatcaaagaa
     6481 gttgatcagc aggcctccac cgggggccgc cgcgctatct ctatcgtcac cgaaacccgc
     6541 aatttccacg caatcggcgt acggcttggt cgtcacgacg ccaccatcac cctgtttgat
     6601 ctcagcagca aagtgctggc agaagaacat tacccgctgc cggaacgtac ccagcagacg
     6661 ctggaacatg ccctgctgaa tgccattgct cagtttattg atagctacca gcgcaaactg
     6721 cgcgagctga tcgccatttc cgttatcctg ccaggacttg ttgacccgga cagcggcaaa
     6781 attcattaca tgccgcatat tcaggtagaa aactgggggc tggtagaagc actggaagag
     6841 cgttttaaag tgacctgttt cgttggtcac gatatccgta gtctggcgct ggcggagcac
     6901 tacttcggtg caagtcagga ttgcgaagac tccattctgg tgcgtgtcca tcgcggaacc
     6961 ggggccggga ttatctctaa cgggcgcatt tttattggcc gcaacggcaa cgtcggtgaa
     7021 attggccata ttcaggtcga accgctgggt gaacgctgcc actgcggcaa ctttggctgc
     7081 ctggaaacta tcgctgccaa cgccgctatt gaacaacggg tgttgaatct gttaaagcag
     7141 ggctaccaga gccgcgtgcc gctggacgac tgcaccatca aaactatctg caaagccgcg
     7201 aacaaaggcg atagcctggc ctcggaagtg attgagtatg tcggtcgtca tctgggcaaa
     7261 accatcgcca ttgctatcaa cttatttaat ccgcaaaaaa ttgttattgc cggtgaaatc
     7321 accgaagccg ataaagtgct gctccctgct attgaaagct gcattaatac tcaggcgctg
     7381 aaggcgtttc gtactaatct gccggtggta cgttctgagc tagaccaccg ctcggcaatc
     7441 ggtgcttttg cgctggtaaa acgcgccatg ctcaacggta ttttgctcca gcatttgctg
     7501 gaaaattaat gtgcttttat agtggcgctt attgttgtca atattctggg tagtccatga
     7561 ccattaaaaa tgtaatttgc gatatcgacg gcgtgctgat gcacgataac gtcgccgtac
     7621 cgggtgcagc ggaatttttg cacgggatta tggataaagg cctgccgctg gtgttgctga
     7681 ccaactatcc ttcgcagacc gggcaagatc tggcgaaccg ctttgccact gccggtgtcg
     7741 atgtacctga cagcgtgttt tatacctctg cgatggcgac cgccgatttc ctgcgtcgcc
     7801 aggaaggcaa gaaagcgtat gttgtgggcg aaggcgcact gattcatgaa ctgtacaaag
     7861 ccggtttcac tattaccgat gtgaaccctg attttgtgat tgttggcgaa acgcgttcct
     7921 acaactggga catgatgcat aaagcggcct atttcgtcgc taacggtgca cgctttatcg
     7981 ccaccaaccc ggacacccac gggcgcggtt tttatccggc ctgtggcgcg ttgtgtgcag
     8041 ggattgagaa aatctccggg cgcaaaccgt tctatgttgg taagccgagt ccgtggatta
     8101 tccgcgcggc attaaacaaa atgcaggcac attcggaaga aacggtgatt gtcggcgata
     8161 acctgcgtac cgatattctg gccggcttcc aggcaggtct ggagacgatt ctggtgcttt
     8221 ctggtgtttc gtcgctcgac gatatcgaca gtatgccttt ccgccccagc tggatttacc
     8281 cgtcagtcgc tgaaatcgac gttatctgaa tagcaaccac gtctcaggac gtggtttctt
     8341 atttttaccc atcattaaaa tcatcccatt caataaaacc cgccaaaaat tatcgatcca
     8401 tcaataaata catcaccgca ccctcccctt tttcacaaat cagcaatcac cattacgttt
     8461 tttatttttt caccgccaaa tcgcttgcgc acagtgctgc tttgcggcat ttttttaaac
     8521 aagcaaacac aacaagcaac aaataccagg ttaacggaga aggttatgtg ttcaattttt
     8581 ggcgtattcg atatcaaaac agacgcagtt gagctgcgta agaaagcact cgagctgtca
     8641 cgcctgatgc gtcatcgtgg cccggactgg tccggtattt atgccagcga taaagccatt
     8701 ctcgctcacg aacgtctgtc aattgttgac gttaacgcgg gggcgcaacc tctctacaac
     8761 caacaaaaaa cccacgtact ggcggtaaac ggtgaaatct acaatcacca ggcattgcgc
     8821 gctgaatatg gcgatcgtta tcagttccag acagggtctg actgtgaagt gatcctcgcg
     8881 ctgtatcagg aaaaagggcc ggaatttctc gacgacttgc agggcatgtt tgcctttgcc
     8941 ctgtacgaca gcgaaaaaga tgcctacctg attggtcgcg accatctggg gatcatccca
     9001 ctgtatatgg gctatgacga acacggtcag ctgtatgtgg cctcagaaat gaaagccctg
     9061 gtgccagttt gccgcacgat taaagagttc ccggcgggga gctatttgtg gagtcaggac
     9121 ggcgaaattc gttcttatta tcatcgcgac tggttcgact ttgatgcagt gaaagataac
     9181 gtgaccgaca aaaacgagct gcgtcaggct ctggaagatt ccgttaaaag ccatctgatg
     9241 tctgatgtgc cttacggtgt gctgctttct ggtggtctgg attcctcaat tatttccgct
     9301 atcaccaaga aatacgcagc ccgtcgcgtg gaagatcagg aacgctctga agcctggtgg
     9361 ccgcagttac actcctttgc tgtaggtctg ccgggttcac cggatctgaa agcagcccag
     9421 gaagtggcaa accatctggg cactgtgcat catgaaattc acttcactgt acaggaaggt
     9481 ctggacgcca tccgcgacgt gatttaccac atcgaaacct atgatgtgac cactattcgc
     9541 gcttcaacac cgatgtattt aatgtcgcgt aagatcaaag cgatgggcat taaaatggtg
     9601 ctgtccggtg aaggttctga tgaagtgttc ggcggttatc tttacttcca caaagcaccg
     9661 aatgccaaag aactgcatga agagacggtg cgtaaactgc tggccctgca tatgtatgac
     9721 tgcgcgcgtg ccaacaaagc gatgtcagcc tggggcgtgg aagcacgcgt tccgttcctc
     9781 gacaagaaat tcctcgacgt ggcgatgcgc attaacccgc aggataaaat gtgcggtaac
     9841 ggcaagatgg aaaaacacat cctgcgtgaa tgttttgagt catacctgcc cgcaagcgtg
     9901 gcctggcggc agaaagagca gttctccgat ggcgtcggtt acagttggat cgacaccctg
     9961 aaagaagtgg cggcgcagca ggtttctgat caacagctgg aaactgcccg cttccgcttc
    10021 ccgtacaaca caccgacctc aaaagaagcg tatctgtacc gggagatctt tgaagaacta
    10081 ttcccgcttc cgagcgccgc tgagtgcgtg ccgggcggtc cttccgtcgc gtgttcttcc
    10141 gctaaagcga tcgaatggga tgaagcgttc aagaaaatgg acgatccgtc tggtcgcgcg
    10201 gttggtgttc accagtcggc gtataagtaa gaaatgcgaa acagccccgg tgaaatccgg
    10261 ggctgtttca gttattgagg tgcttgttga ttctgatatg cagtaaggaa atgttccggg
    10321 tttgccacat cactgtttga caactcatca ctgtaggcca gtatttcata gattaatttc
    10381 ccaccactat tgttaaattc cgaatttcat cctgtatgac aaaagaatgg ctgtagggtg
    10441 ggaaaaaaca caaagtaaac aataattgac gaatatagcg ccacgctgtt cgcaacctaa
    10501 ccaaacagtc actttcgagc aattttcctt gaaaaagagg ttgacgctgc aaggctctat
    10561 acgcataatg cgccccgcaa cgccgataag gtatcgcgaa aaaaaagatg gctacgtagc
    10621 tcagttggtt agagcacatc actcataatg atggggtcac aggttcgaat cccgtcgtag
    10681 ccaccatctt ttttttgcgg gagtggcgaa attggtagac gcaccagatt taggttctgg
    10741 cgccgcaagg tgtgcgagtt caagtctcgc ctcccgcacc attcaccaga aagcgttgta
    10801 cggatggggt atcgccaagc ggtaaggcac cggtttttga taccggcatt ccctggttcg
    10861 aatccaggta ccccagccat cttctttgag taagcggttc accgcccggt tattggggta
    10921 tcgccaagcg gtaaggcacc ggtttttgat accggcattc cctggttcga atccaggtac
    10981 cccagccatc gaagaaacaa tctggctacg tagctcagtt ggttagagca catcactcat
    11041 aatgatgggg tcacaggttc gaatcccgtc gtagccacca aattctgaat gtgtcgaata
    11101 cgttcggcaa attcaaaaac caatttgttg gggtatcgcc aagcggtaag gcaccggatt
    11161 ctgattccgg cattccgagg ttcgaatcct cgtaccccag ccaatttatt caagacgctt
    11221 accttgtaag tgcacccagt tggggtatcg ccaagcggta aggcaccgga ttctgattcc
    11281 ggcattccga ggttcgaatc ctcgtacccc agccacatta aaaaagctcg cttcggcgag
    11341 ctttttgctt ttctgcgttc actcaatgtc gaatgcgatg ttgacacgtc ttatccttca
    11401 atgtcggatg cgacgctgcc gcgtcttatc cgacctacgg ttggcacgca tccggcaatg
    11461 ttgtaaggct acaaccctaa cgcatatttc agcgcctgac gtttcaacac gccagcacgc
    11521 tccgccgcca tcaaaccaag attacgcaca aaacgcagcg gcggcagatt attactgaat
    11581 ccggcataaa acagatccat accgctctgc ataatgaagt tatccgccat gcgccgcatc
    11641 tggtaacgct tgaggacagg ataactggcc cacgcttcgc cgtagctgcg ggcattcacc
    11701 agaacatcaa tcagggcatc gacatcacga taaccaagat tcaccccctg ccccgccagc
    11761 ggatggatgg tgtgcgcggc atcacccacc agcgcaagcc ccggctgcac atactgcaac
    11821 gcatggcggc gtgtcagcgg aaacgcacca gcggcaagcg gtgtaacgta acccagacgc
    11881 gacgggaaat gcttcgcgat ttccacctgt aactgcgcca tattcatatt ctgcaactgg
    11941 cgaatgcgcg ccggagagtc gtaccacacc agcgatgccc agttatcaaa cagcggcaga
    12001 aacgcgcgcg gtccatccgg ggtaaattgc tgccaggtgc tatcgccggg atcgttctcg
    12061 cactggacgc taatcaacat gcacgactgc gcatactgcc atgcatgaac gccaattccc
    12121 gccatctgcc gcacctgcga atttgcgccg tcggcaccaa tcaccagctt cgcgcgaatc
    12181 acttcaccgc ctttcagctc cagctcctga agatcattat ctcgatgtaa tgcaatcagc
    12241 gaggttggca cacgtaacgt tactttcgga tgcgcttcca gcgcctgcca caatgcctgt
    12301 tgcaggacag tgttttctac catatagcca agcaacggca actttaattc ggctgcgtca
    12361 aataccacat gcgccgtttc ccactcccac gtttccagtc tgcggtaagg atggcaacgc
    12421 atagcctgta ctgcatccca gacccctaac cctttaagca atgataccga agccgcgctg
    12481 atcgccgaga tccgcacgtc cggttggcta tcagcgacaa acggtgcagg ttctgcgtgc
    12541 tcgatcaccg ttaccgtaaa tccgtgctgt gccagcccca gcgccagtgc gccgccgacc
    12601 attcctccgc cgacaatggc aatttccgtt ggttgatttg tcatggtcaa tcatcctgtt
    12661 agcaatatgc tttaagttta caggattttt acccccaagg gctgataacg ctcatactgg
    12721 tcacaacggc agcaaagcat tacactatgc gccctgcatt cctggctact atttcgcaag
    12781 agcaagtcga tgaccaaaaa actccatatt aaaacctggg gctgtcagat gaacgagtac
    12841 gattcatcga agatggccga tctgctggat gccacccacg gctatcaact gaccgacgtg
    12901 gcggaagaag cggatgtgct gctgctgaac acctgctcaa tccgcgagaa ggctcaggaa
    12961 aaagtcttcc atcagttggg tcgctggaaa ctgttaaaag agaagaatcc agacctgatt
    13021 atcggcgtcg gtggctgcgt ggcatcgcaa gaaggcgagc acattcgcca gcgcgcccac
    13081 tatgtcgata ttatttttgg gccgcaaacg ctgcaccgtc tgccggagat gatcaactcc
    13141 gtgcgcggcg accgcagccc ggttgtagat atcagcttcc cggaaatcga gaagtttgac
    13201 cgtctaccgg aaccgcgcgc cgaagggccg accgcgtttg tctccatcat ggaaggctgc
    13261 aataaatatt gcacctactg cgtggtgcct tacacccgtg gtgaagaggt aagccgtccg
    13321 tctgacgata ttctgtttga gattgcccag cttgcggctc agggcgtgcg tgaagtcaac
    13381 ctgctcggtc agaacgtgaa cgcctggcgt ggtgaaaact acgacggcac caccggatcg
    13441 tttgccgatc tgttgcgtct ggttgctgcg atcgacggga tcgatcgtat tcgctttacc
    13501 accagccatc cgatcgaatt caccgacgat atcatcgaag tgtatcgcga cacgccggag
    13561 ctggtgagct tcctgcatct gccggtacag agcggttccg atcgcattct gaacctgatg
    13621 ggacgtaccc atacggcgct ggagtacaaa gcgatcatcc gtaaactgcg tgcggcgcgt
    13681 ccagatattc agatcagttc tgacttcatc gtcggcttcc ctggcgaaac caccgaagac
    13741 ttcgagaaaa cgatgaagct gattgccgac gtcaatttcg acatgagcta cagctttatc
    13801 ttctctgcac gtccgggtac accagccgcc gatatggttg atgatgttcc ggaagaagag
    13861 aagaagcagc gtctgtatat tctgcaagaa cgtatcaatc agcaagcgat ggcgtggagc
    13921 cgccggatgc tcggcaccac ccagcgtatt ctggtagaag gtacatcgcg taagagcatc
    13981 atggagcttt ccgggcgtac ggaaaataac cgcgtggtca acttcgaagg cacgccggat
    14041 atgatcggta aattcgtcga tgtagaaatt accgacgtct acccgaactc tctgcgcggt
    14101 aaagtggtgc gtactgaaga tgaaatgggt ctgcgcgtgg cagaaacacc agaatcggtg
    14161 attgcccgta ctcgcaaaga aaacgacctt ggcgtgggtt attatcagcc gtaattctca
    14221 ggcccgccgt tccggtgggc cttgtttttt ctctctttat ccccatcttt atcgcaatgc
    14281 ttgcgccttt ttcctgtggc gtgaataatt tccttatagg ccggtgaatg ttcagcttcc
    14341 ggcggcatac agataagagg aacggtttga acatagacac tcgcgaaatc accctggagc
    14401 cagcagacaa cgcgcgtctg ttgagcctgt gcggcccgtt tgatgacaac atcaagcagc
    14461 tcgaacgccg tctcggcatc gagatcaatc gccgcgataa ccactttaaa ctgaccggcc
    14521 gtccgatttg cgtcaccgct gcggcagaca ttctgcgtag cctgtatgtc gatactgccc
    14581 cgatgcgcgg tcagattcag gatatcgaac cggaacagat ccaccttgcg attaaagaag
    14641 cacgggtact ggagcaaagc gcggagagcg tgccggagta cggcaaagcg gtcaatatca
    14701 aaaccaaacg cggcgtaatt aagccgcgca cgccaaacca ggcgcagtac atcgccaata
    14761 ttctcgacca tgacattacc ttcggcgttg gcccggcggg tacgggtaaa acctacctgg
    14821 cagtggctgc ggcagttgat gccctggagc gtcaggaaat tcgccgtatt ctgctgactc
    14881 gtccggcagt agaagccggt gagaaactgg gcttcctgcc tggcgattta agccagaaag
    14941 tagacccgta tctgcgcccg ctgtacgacg cgctgtttga aatgctgggt tttgagaaag
    15001 tcgagaaact gattgagcgc aacgttattg aagtcgcacc gctggcctat atgcgtggtc
    15061 gtacgctgaa cgacgcattt atcattctcg atgagagcca gaacactacc atcgaacaga
    15121 tgaagatgtt cctgacccgt atcggtttta actcaaaagc ggttatcacc ggcgacgtca
    15181 cacagatcga cctgccgcgt aatactaaat caggcttacg tcatgccatc gaagtgctgg
    15241 ccgatgtcga agagatcagc tttaacttct tccacagcga agacgtggtt cgtcacccgg
    15301 tggtggcgcg tatcgttaac gcctatgaag cctgggaaga agccgaacaa aaacgtaaag
    15361 cggcgctggc ggcagaacgc aagcgcgaag aacaggaaca aaaatgagtc aggtgatcct
    15421 cgatttacaa ctggcatgtg aagataactc cggattgccg gaagagagcc agtttcaaac
    15481 atggctgaat gcggtgatcc cgcagtttca ggaagagtcg gaagtgacga ttcgcgtggt
    15541 cgataccgcc gaaagccaca gtctcaatct gacctatcgc ggtaaggata agccgaccaa
    15601 cgtgctctcc ttcccgtttg aagtgccgcc tggcatggag atgtcgctac tgggcgatct
    15661 ggttatctgc cgtcagatgg ttgagaagga agctcaggag caaggcaaac cactggaggc
    15721 gcactgggcg catatggtgg tgcacggcag tctgcatttg ttaggttacg atcacatcga
    15781 agatgacgaa gcagaagaaa tggaagccct cgaaacagag attatgcttg ctctgggcta
    15841 tgaggatccg tacattgccg agaaagaata atttgccagc catttgactg gcagacgtcc
    15901 ccgccgttgg tgattaacga cggcgttttg attaactaac atgagaaccc atacgacgcc
    15961 atgagcgacg acaattcaca cagtagtgac acgataagca acaagaaggg atttttctcc
    16021 ctgttactca gccaactttt ccacggtgaa ccgaaaaacc gtgacgaact gctggcgctg
    16081 atccgtgatt ccgggcagaa cgaccttatc gacgaagata cgcgcgatat gctcgaaggg
    16141 gtgatggaca tcgcagacca acgcgtccgc gacatcatga tcccccgctc ccagatgatt
    16201 accctgaaac gcaaccagac gctggacgaa tgccttgatg tcatcatcga gtccgcccac
    16261 tcacgtttcc cggtgattag cgaagacaaa gatcacattg aagggattct gatggcgaaa
    16321 gacttgctgc cgtttatgcg cagcgatgct gaagccttca gcatggacaa agtgttacgt
    16381 caggcggttg tcgttcctga aagtaagcgc gtagaccgga tgctgaaaga gtttcgctct
    16441 cagcgttacc acatggcgat cgttattgac gaattcggtg gggtttccgg tctggtaacc
    16501 attgaagaca tcctggaact gattgttggt gagattgaag acgaatatga cgaagaagat
    16561 gatatcgact tccgtcagct gagtcgtcat acctggaccg tgcgcgcact ggcttccatt
    16621 gaagacttca acgaagcgtt cggcacccac tttagcgatg aagaagtcga cactatcggt
    16681 ggtctggtga tgcaggcatt tgggcatctt ccggcacgtg gcgaaaccat cgacatcgac
    16741 ggttaccagt tcaaagtggc gatggccgac agtcggcgta ttattcaggt tcatgtcaaa
    16801 atcccggatg actcacccca gccgaagctg gatgaataaa accgaaactg gatagataac
    16861 tacatggctt ttgcctcatt aattgaacgc cagcgcattc gcctgctgct ggcgttatta
    16921 ttcggtgcct gcggaacgct ggccttctct ccttacgacg tctggcctgc ggcgattatt
    16981 tcgctgatgg ggcttcaggc gttgaccttt aaccgccgtc cactccagtc tgccgctatt
    17041 ggcttttgct ggggatttgg cctctttggc agcggtatta actgggtcta tgtcagcatc
    17101 gcgacctttg gcggaatgcc tggcccggtt aacatcttcc tggtggtact gctggcggcg
    17161 tatttgtcgc tgtataccgg actgtttgct ggcgtgctgt cgcgcctgtg gccgaaaacc
    17221 acctggctgc gcgtggcgat tgccgcccct gctctctggc aagtgaccga gtttctgcgc
    17281 ggttgggtac tgacaggctt cccgtggtta cagttcggct atagccagat tgatggcccg
    17341 ttaaaagggc tggcaccgct aatgggcgtg gaagccatta acttcctgct tatgatggtt
    17401 agcggcctgc tggcactggc gttagtcaaa cgcaactggc gtccgctggt ggtggccgtc
    17461 gtgctgtttg cccttccctt cccgctgcgt tacatccggt ggtttacccc acaaccggag
    17521 aaaaccattc aggtttcgat ggttcagggc gatattccgc aatcgctgaa atgggacgaa
    17581 ggccagcttc ttaatacgct gaagatttac tacaacgcaa cggcaccgct gatgggcaaa
    17641 tcatcgttga ttatctggcc ggagtcggcg ataaccgatc tggaaattaa tcagcaaccg
    17701 ttcctcaaag cactggacgg tgagttgcgt gataaaggta gctcgctggt gaccgggatt
    17761 gttgacgcgc gtcttaataa gcagaaccgt tacgatacct acaacaccat catcacactg
    17821 ggtaaaggtg caccgtacag ctacgaatca gccgatcgct ataacaaaaa ccatctggtg
    17881 ccgtttggcg agtttgtccc gctggagtcg attctgcgtc cgttagcacc gttctttgat
    17941 ctgccgatgt cgtcgttcag ccgtgggcca tatatccagc cgccgctgtc ggcaaatggt
    18001 attgagctta ctgcggctat ttgctacgag atcattctcg gcgagcaagt gcgcgataac
    18061 ttccgcccgg ataccgacta tctgctgact atctccaacg atgcgtggtt tggtaagtct
    18121 attggtccat ggcagcactt ccagatggcg cgaatgcgtg cgctggagct ggcgcgccca
    18181 ctgttgcgca gcaccaacaa cggcattacg gcggtgattg gcccgcaggg tgagattcag
    18241 gcgatgatcc cgcagttcac ccgcgaggtg ttaaccacta acgtgacgcc gaccaccgga
    18301 ctcacaccat acgcacgtac cggcaactgg ccgctgtggg tgctgacggc actgtttggt
    18361 tttgctgctg tgttgatgag tctgcgtcag cgacgtaaat aatcccttct tatcgtgccg
    18421 gaatacatcc catatttcgg cacgctcacc ggatgcggca tcagtgcatt atccggataa
    18481 caatattctc ctatccaact ttggcacatc tattgctttg ttatacaagg caaagcttta
    18541 accagcatca gtacaacccg gttgcaccac agcaatccac cggtagcacc aaaagagtgc
    18601 attttaagaa ttttgcttcg gaatggtgcg gcgcacctcc caaatataaa caataaaaca
    18661 gcaatatctt tacatttagc caaactaaat gttacctaac aggcacaaca ctacacaata
    18721 aagttgcaga tgataacaac acaaacactc acaacgggta tccatgcgtt cttaacgcag
    18781 aagataaagg agttggatat gcaattacgt aaacctgcca cagcaatcct ggccctggcg
    18841 ctttccgcag gactggcaca ggcagatgac gccgccccgg cagcgggcag tacgctggac
    18901 aaaatcgcca aaaacggcgt gattgtcgtc ggtcaccgtg aatcttcagt acctttctct
    18961 tattacgaca atcagcaaaa agtggtgggt tattcgcagg attactccaa cgccattgtt
    19021 gaagcagtga aaaagaaact caacaaaccg gacttgcagg tgaaactgat tccgattacc
    19081 tcacaaaacc gtattccgct gctgcaaaac ggcacttttg attttgaatg tggttctacc
    19141 accaacaacg tcgaacgcca aaaacaggcg gctttctctg acactatttt cgtggtcggt
    19201 acgcgcctgt tgaccaaaaa gggcggcgat atcaaagatt ttgccgacct gaaaggcaaa
    19261 gccgtagtcg tcacttccgg cactacctct gaagttttgc tcaacaaact gaatgaagag
    19321 caaaaaatga atatgcgcat catcagcgcc aaagatcacg gtgactcttt ccgtaccctg
    19381 gaaagcggtc gtgccgttgc ctttatgatg gatgatgctc tgctggccgg tgaacgtgcg
    19441 aaagcgaaga aaccagacaa ctgggatatc gtcggcaagc cgcagtctca ggaagcctat
    19501 ggttgcatgt tgcgtaaaga cgatccgcag ttcaaaaagc tgatggatga cactatcgct
    19561 caggtgcaga cctccggtga agcggaaaaa tggtttgata aatggttcaa aaatcccatt
    19621 ccgccgaaaa acctgaacat gaatttcgaa ctgtcagacg aaatgaaagc actgttcaaa
    19681 gaaccgaatg acaaagcact gaactaatta caagaaccag gggcggaaat tctagccctc
    19741 tcgattgtta cgtagcacgg acagactata cgcctgatgg tcgttcccca tcgggcctga
    19801 aaaccgcaat acgctgggta acaatcttcg agggtagcag ttaacgctgc taccctcttt
    19861 tttctggagt agatttatgt ctatagactg gaactggggt atttttttac aacaagcccc
    19921 gttcggcaac accacctatc ttggttggat ctggagtggt tttcaggtga cgatcgcttt
    19981 gtcgatctgc gcctggatta tcgctttcct cgtcggttca tttttcggta ttttacgtac
    20041 cgttccaaac cgttttctct ccggtctggg tacgttgtat gtcgaactgt tccgcaacgt
    20101 gccgttgatt gtgcaattct ttacctggta tctggtgatc ccggagctgc tgccggagaa
    20161 aatcggcatg tggtttaagg ccgagctgga tcccaatatt cagtttttcc tttcatccat
    20221 gctctgcctg gggctgttta ccgccgcccg tgtttgcgaa caggtacgcg ccgcgattca
    20281 gtcgctgccg cgtgggcaga agaatgccgc gctggctatg ggtctgacgc tgccgcaggc
    20341 ttaccgctac gtactgctgc ctaatgctta tcgcgttatc gtcccgccga tgacctcaga
    20401 gatgatgaac ctggtgaaaa actccgccat cgcctcgacc atcggtctgg tagatatggc
    20461 agcgcaggcg agtaaattgc tcgattactc ggctcacgcc tgggagtcgt ttaccgccat
    20521 tacgctagcg tatgtgttta ttaacgcttt tatcatgctg gtgatgacgc tggttgaacg
    20581 taaagttcgc ctgcctggca acatgggggg caaataatgt acgagtttga ctggagttcc
    20641 attgtccctt ccctgccata tctgctcgac gggctggtga tcacgctgaa aatcaccgtc
    20701 acggcagtcg tgattggtat tttgtggggc accatgctgg cggtgatgcg cttgtccagc
    20761 tttgcgcccg tagcgtggtt tgccaaagcc tacgttaacg tattccgctc gattccttta
    20821 gttatggttt tgctgtggtt ttacctgatc gtgccgggtt ttctgcaaaa cgtgctggga
    20881 ttatcaccaa aaaatgatat ccgtttaatt tcggcgatgg tggcgttttc gatgtttgaa
    20941 gcggcctact attcagagat tatccgtgcc ggtattcaaa gtatttctcg tggtcagtcg
    21001 agcgccgcgc tggcgttggg aatgactcac tggcagtcga tgaaactgat tattctgccg
    21061 caggcgttcc gcgcgatggt gccgctgctg ctcactcagg gcatcgtact gttccaggat
    21121 acctcgctgg tgtatgtgtt atctctggcc gatttcttcc gtaccgcctc aaccattggt
    21181 gagcgtgatg gcacgcaggt tgagatgatc ctgtttgccg gatttgttta tttcgttatt
    21241 agccttagcg cgtcgttgtt ggtcagctac ttgaaaagaa ggacagcata atgattaccc
    21301 tgaaaaatgt ttcaaaatgg tatggtcact ttcaggtgct gaccgactgc tcaaccgaag
    21361 tgaaaaaagg cgaagtggtg gtggtttgcg gcccgtctgg ttccggcaaa tcaacgctga
    21421 ttaaaaccgt caacggcctc gaaccagtgc agcaaggtga aatcaccgtc gatggtatcg
    21481 tggttaacga caagaaaacc gatctggcaa agctgcgttc tcgcgtcggg atggtattcc
    21541 aacatttcga gctgttccct catctgtcga ttatcgaaaa cctgaccctg gcgcaggtga
    21601 aagtgcttaa acgcgataaa gcaccggctc gtgaaaaagc attgaaattg ctggagcgcg
    21661 tgggactttc cgcacatgcc aataaatttc cggcgcagct ttccggcggc cagcagcagc
    21721 gtgtggcaat tgctcgcgca ttgtgtatgg atcctattgc gatgctgttc gacgaaccca
    21781 catcggcact agacccggag atgatcaacg aagtactgga cgtgatggtg gaactggcga
    21841 acgaggggat gaccatgatg gtggtgaccc acgaaatggg ctttgcccgt aaagtggcga
    21901 atcgggtgat ctttatggat gagggtaaaa ttgttgaaga ctcgccgaaa gatgcgttct
    21961 tcgatgatcc gaaatcggac cgcgcaaaag acttcctcgc gaaaatcctg cattaatcac
    22021 tctggcgcgc atacccaagt gcgcgccatc tcacgcttta tcgtctcgcc cctcgtatct
    22081 gttacatcct ggcgttaccc ttgtcgcaaa gaagcacaca acaaggagca acaatggcac
    22141 tgccaattct gttagattgc gacccaggtc atgatgacgc tatcgcaata gttctcgccc
    22201 tcgcctcacc agagcttgat gtcaaagcaa ttacgtcttc cgccggaaac cagacaccag
    22261 aaaaaacctt acgcaatgtt ctgcgtatgc tgaccttgct taatcgcacc gatattccgg
    22321 tggcaggcgg cgcggtaaaa ccgttaatgc gtgagttgat tatcgcggac aatgtgcacg
    22381 gcgaaagcgg tctcgacggc ccggcattac cggaaccgac gttcgcaccg caaaactgta
    22441 cggcggtaga gctgatggcg aaaacgctgc gtgaaagtgc ggaacctgtc acgattgtgt
    22501 ctaccggacc gcaaactaac gttgccttgc tgctcaatag ccatccggaa ctgcatagca
    22561 aaattgcccg tatcgtgatt atgggtggcg ctatgggact gggtaactgg acgcctgcgg
    22621 ctgaatttaa tatttacgtt gacccggaag cggcagaaat tgtcttccag tcagggatcc
    22681 cggtggtgat ggccggtctg gatgttactc acaaagcaca aatccacgtt gaagacactg
    22741 agcgtttccg cgcgattggc aaccctgttt caaccattgt tgccgaactg ctggatttct
    22801 tcctcgaata tcataaagac gaaaaatggg gctttgtcgg cgcaccgcta catgacccat
    22861 gcaccatcgc ctggctgtta aaaccggagt tgtttaccac tgttgagcgc tgggttggcg
    22921 tggaaacaca ggggaaatat acccagggta tgacggttgt tgattattat tatctgactg
    22981 gcaataaacc gaatgccact gtaatggtcg atgttgatcg tcagggcttt gttgatttac
    23041 tggctgatcg tctgaaattt tacgcttaac acacgtcatt gattaagatc accacgcgtt
    23101 acctgggtgg tcttaatctg tttatctctt tttctttcaa gatataaatg caatggataa
    23161 tgcagaactc gccattggta tcgatctcgg tactaccaat agtttaattg ccgtctggaa
    23221 agacggtgcc gcgcaattaa ttccaaataa gttcggtgaa tatttaacac catccataat
    23281 tagcatggat gaaaataatc atattttagt cggaaaaccg gctgtatcac ggcgtacttc
    23341 gcatccggat aaaacggcag cgttatttaa acgtgcaatg ggcagtaata ccaactggcg
    23401 gttaggcagc gacacattta acgcgccaga actgtcctct ttggtattac gctcattaaa
    23461 agaagatgcc gaagaatttc tgcaacgtcc gattaaagat gtggtgatct ctgttccggc
    23521 ttatttcagc gatgaacaac gcaagcatac ccgtttagca gcggagttag ccggtttaaa
    23581 tgcggtacgc ttaattaatg aacccacagc agctgcgatg gcgtatggcc tgcataccca
    23641 acaaaatacc cgttcgctgg tgtttgatct cggtggcggc acgtttgacg tcacggtgct
    23701 tgagtacgcc acgccggtga ttgaagttca cgcctccgct ggcgacaact tcctcggtgg
    23761 cgaagatttt acccatatgc tggtcgatga ggttttaaaa cgcgcggatg tcgccaggac
    23821 cacgcttaac gagagtgaac tggcagcctt gtatgcctgt gtggaagcgg caaaatgtag
    23881 caatcaatcg ccaatacaca ttcgctggca gtaccaggat gaaacgcggg aatgcgaatt
    23941 ttacgagaac gaactggaag atttgtggtt gccgctgctc aatcgcttgc gagtgccgat
    24001 tgaacaggcg ttgcgcgatg cgcgtctgaa gccgagtcaa atcgacagtc tggtgctggt
    24061 tggcggcgcg tcacaaatgc cgctggtgca gcgaatcgcc gtgcgtctgt ttggcaaatt
    24121 accgtatcaa agttacgatc cgagcaccat tgtcgcgctg ggcgcagcaa tccaggcagc
    24181 ctgccgctta cgcagtgaag atattgaaga agtgatcctc actgatattt gcccttactc
    24241 gttgggcgtt gaggttaacc gccagggcgt ttccggcatt ttctcgccga ttattgaacg
    24301 aaacaccact gtgcccgtgt cgcgtgtaga aacttattca accatgcacc cggaacagga
    24361 ttctattacg gttaacgtct atcagggaga aaaccacaaa gttaaaaaca acattctggt
    24421 ggaatccttc gatgtgccgt tgaagagaac cggggcttat cagtcgattg atattcgctt
    24481 tagttatgat atcaacggtt tgcttgaagt tgacgtgctt ctggaagacg gcagcgttaa
    24541 gtccagagtg attaaccaca gcccggtaac attgagcgcg cagcagattg aagagagtcg
    24601 gacgcggtta tccgcattga aaatttatcc gcgcgatatg ctcatcaacc gcacctttaa
    24661 ggccaaactg gaagagttgt gggcgcgggc gctgggtgac gagcgagaag agattggccg
    24721 ggtgatcacc gattttgatg cggcgctgca gtcaaacgat atggcccgcg tcgatgaggt
    24781 caggcggcag gcgagcgttt atttagccat tgagatccca taatcacggc tgccggatgc
    24841 ggcgtaaatg ctgcatccgg catcggcgca aaaaacacat cgtcaactaa acaacatcgc
    24901 gattttgccg agagcataac ctatcaccag caccgttaag ctgattttga taatgctggt
    24961 aatgctctgt ggtttttcat cctcatcatt tttttcttca tggttttcag gtgttggctc
    25021 atcgacatct tcatcctccg gctcgagttt tactttcgtc aacagcatcc gggtgataat
    25081 ggcctctaat ggatgagcat ctccgccttc cagccttgta ttaagacggg ccagcatctc
    25141 ttgtttcgga aacgcttcaa gtactacctg ttccgctacc cgatacaaat ccagtaacgg
    25201 tgaatcttct tctgtctcgc taccgactct cgatttccac cacgcccatg ctttgcgttt
    25261 gaagaatagc gactcaataa acgattgacc taacggcgat agttttccgc tatgtaaaat
    25321 ttccgcccat aacagacgcg tccgggcgct ctcaccagga acatatttca acggtttacc
    25381 ttgcgcatct accatgctcc gcgcttcgat aaccatcact aataacgcga aataatccgg
    25441 gcaatgttgt gcgctccact gcaacaacaa attatcggct tgtgtagatg ggtaacttcc
    25501 ccagtacgcg cagagatcgg taagcaggct ttccccctcg ccgcagtaca aacgttgtgc
    25561 acactggtaa taccgggcat cttcgctttc tggttcttcc atttgccact gttcggctac
    25621 aggaatgagt tccgccatac ctgtttgcac cgagctgtac cagcgcaaca gtttgcgatg
    25681 taactttgca tcatcaggaa tgaaccacgg accttccatc gccaaaaacg cagtgacata
    25741 gtcaggatgg taacggcagc aacgttccag cgcgaagtaa tactcaacag tctggttctg
    25801 caacgcaacg ggatatacca gaaggccaag gaaatcgaaa caatcaccag cctgaatggc
    25861 ctcaagaaaa gcctcccttt cttactcatc tattccttcg ctgtcattaa acgaatgcca
    25921 gttcaaacgc tctgccagca aagaagcaca atttaatgaa agatatcgcg cttctatagc
    25981 gattgcacac agcggccagc gtaattgatc gacatcctcc atgttccagg tatttaattg
    26041 ctgaataaat ttctgccagg cggagggctg aaaacgatca ctttctgaat ccagtaatgc
    26101 cctgaataca cgtagtattt catgctcagt ggcatcatct ttttcttcat catctgcttc
    26161 ctcgacagga tttaccgcca gtcgcagggc ctcttcatag gcctggcgta atgctttaaa
    26221 cccttgcgga tcggtttcgg gatgacataa cggtaagcga gcaagataag cctggcggat
    26281 aatgtctatt tgcgtcgtgc tttcaatttc gaggatttgc caacatgttt tcataggtcg
    26341 ctcacatcct taagcgttac cgggcattca ggctggacag ttaatggctg ccagggaagc
    26401 gtggcataga aacgatcgct tcccgcagcc agctcttcga acatccgact cccggcgcta
    26461 tcgtatttgt acagggttag cgcctcgcgt agcgaagcgt tgtcttcgca ttgagaagat
    26521 tgccagtaga gacgccctag tgaataggcg gaaaaatagt gcatccagct gtgatagtaa
    26581 tggtgagctc gttcatagac gcgcgattgc agccataaac tctcgtcttc cgtcagccac
    26641 ttgttgagca caccgacccg tgacagaaac cccattcgaa cataatccca agctttgatt
    26701 cccccttccc cacaacacat agcggtttgg gcaacgaatt gggtataaat caaccctctc
    26761 tccgagccac cagcaattaa tgctttccac tcttcagggg aagagcgatg ccatgtcaga
    26821 tataagccag ctaaatcatt ggcatgacca tcatcagtca tctgataaat agtctgaatt
    26881 aaatcatcac gattatttat tccccaggat gatgagagat cgaattcttt tatcccggta
    26941 tacatttttg gcgagtcatg cgcgggtttg caaccctgat taagtatttc catgggcgca
    27001 gaaagcgcaa aaagaagata ttgttcttcc ttatt
//
LOCUS       DBFIFM010000045        26518 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.045, whole genome shotgun sequence.
ACCESSION   DBFIFM010000045 DBFIFM010000000
VERSION     DBFIFM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.045"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(118..2988)
                     /gene="xdhD"
                     /locus_tag="N7C74_001667"
     CDS             complement(118..2988)
                     /gene="xdhD"
                     /locus_tag="N7C74_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HGU2431551.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIEVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVDMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(2985..3764)
                     /gene="ygfM"
                     /locus_tag="N7C74_001668"
     CDS             complement(2985..3764)
                     /gene="ygfM"
                     /locus_tag="N7C74_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HGU2431552.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(3814..5142)
                     /gene="ssnA"
                     /locus_tag="N7C74_001669"
     CDS             complement(3814..5142)
                     /gene="ssnA"
                     /locus_tag="N7C74_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HGU2431553.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPSPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVN
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(5145..8243)
                     /gene="ygfK"
                     /locus_tag="N7C74_001670"
     CDS             complement(5145..8243)
                     /gene="ygfK"
                     /locus_tag="N7C74_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HGU2431554.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVHGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQDFHYVLIATGTDKNSGVKLAGDNQNVLKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(8565..9143)
                     /gene="mocA"
                     /locus_tag="N7C74_001671"
     CDS             complement(8565..9143)
                     /gene="mocA"
                     /locus_tag="N7C74_001671"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HGU2431555.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIKRPNITNMRQALLMG
                     AHYSVEVENAEIILDIDTPDDFITAQKRYTKI"
     gene            9246..10016
                     /gene="yqeC"
                     /locus_tag="N7C74_001672"
     CDS             9246..10016
                     /gene="yqeC"
                     /locus_tag="N7C74_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HGU2431556.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANLGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            10064..11689
                     /gene="yqeB"
                     /locus_tag="N7C74_001673"
     CDS             10064..11689
                     /gene="yqeB"
                     /locus_tag="N7C74_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HGU2431557.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIIDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFA
                     AGKDCHAVIETNRGHWLGQVIYCGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(11730..12662)
                     /gene="arcC"
                     /locus_tag="N7C74_001674"
     CDS             complement(11730..12662)
                     /gene="arcC"
                     /locus_tag="N7C74_001674"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGU2431558.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVVTLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVEAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(12710..14095)
                     /gene="hyuA"
                     /locus_tag="N7C74_001675"
     CDS             complement(12710..14095)
                     /gene="hyuA"
                     /locus_tag="N7C74_001675"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HGU2431559.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(14148..15359)
                     /gene="ygeY"
                     /locus_tag="N7C74_001676"
     CDS             complement(14148..15359)
                     /gene="ygeY"
                     /locus_tag="N7C74_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HGU2431560.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(15417..16613)
                     /gene="dpaL"
                     /locus_tag="N7C74_001677"
     CDS             complement(15417..16613)
                     /gene="dpaL"
                     /locus_tag="N7C74_001677"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HGU2431561.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(16671..17858)
                     /gene="ygeW"
                     /locus_tag="N7C74_001678"
     CDS             complement(16671..17858)
                     /gene="ygeW"
                     /locus_tag="N7C74_001678"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HGU2431562.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            18337..20115
                     /gene="ygeV"
                     /locus_tag="N7C74_001679"
     CDS             18337..20115
                     /gene="ygeV"
                     /locus_tag="N7C74_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGU2431563.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLYRVAG
                     TGAYGKFLGRQLSGNSRLLCHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVTEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(20155..20634)
                     /gene="xdhC"
                     /locus_tag="N7C74_001680"
     CDS             complement(20155..20634)
                     /gene="xdhC"
                     /locus_tag="N7C74_001680"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HGU2431564.1"
                     /translation="MNHSETITIECTINGMPFQLHAVPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(20631..21509)
                     /gene="xdhB"
                     /locus_tag="N7C74_001681"
     CDS             complement(20631..21509)
                     /gene="xdhB"
                     /locus_tag="N7C74_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HGU2431565.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDSITQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHVGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(21520..23817)
                     /gene="xdhA"
                     /locus_tag="N7C74_001682"
     CDS             complement(21520..23817)
                     /gene="xdhA"
                     /locus_tag="N7C74_001682"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HGU2431566.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWEELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYVSHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNASREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            24233..24988
                     /gene="actS"
                     /locus_tag="N7C74_001683"
     CDS             24233..24988
                     /gene="actS"
                     /locus_tag="N7C74_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HGU2431567.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            25067..25140
                     /locus_tag="N7C74_001684"
     tRNA            25067..25140
                     /locus_tag="N7C74_001684"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25099..25101,aa:Gly,seq:ccc)
     gene            25374..26518
                     /locus_tag="N7C74_001685"
                     /pseudo
     CDS             25374..26518
                     /locus_tag="N7C74_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
BASE COUNT         6402 a         6512 c         6815 g         6789 t
ORIGIN      
        1 aaattcccac gaatttgggg cgcaggacgc cttgagaacg gcttatctca ttttcgcagt
       61 cactatttag tcatcgtcca aacagagaaa caggccgccg aagcggccat ttattattta
      121 tatcttttcc agcgcggtga gtattttctc cggtgtgaaa tgccattcgc gtaaccagat
      181 gccgcatgca tcgtgaattg cggtagcaat cgccggagct gcgccattta caccgatttc
      241 cgagatcgat ttcgccccga acgggccgac tttatcgtcg ctcggcacca gcactgcgcg
      301 gaaatcacgc ggaatgtcac caattttcgg tgcgccgtag ctgcgtaaat cacgcgttaa
      361 cgggtgacct tcggcgtcat aaatgatctc ttcgctcata ctgtggccga tagctcgcag
      421 ggtagcaccg tagatttgtc ccaacgctaa ctctggattg accggtgtac cgcagtccag
      481 caaggcgtag aatttatcca ggcggatttc acccgtacgc gtgttgacgg caacttcagc
      541 gaagtttgcg ccatacggga aggcgaaatc aggcgtgata taactgccag ttcccaccag
      601 tgaaccaaag ccggtgccgg tttcgccttt atgggcaata tccccgaaac taacttcgcc
      661 tttcttgccg cgcacgacgc ccggcgttgc tagttgaaca tctgccactg gctcacccaa
      721 catttgcgcg ccgtggaaca gaattttctc ccgcagattt tccgctgcca aacgcgccgc
      781 gttacccgag aagcaagtac cggacgaggc atatgcgcct ttatcaaaca acgcatgatc
      841 ggtatcaccg gagataacat gcacgtcctg cggtgggcag tgcagcactt ctgctgccag
      901 tttcgtcacc acggtatcca gaccagtacc aatatccgca ccgccagaat gaacgataaa
      961 ggtaccgtct gattccagtt tgatcatgca gttagcctga tcgatatccg ggatccctga
     1021 tttctgcatg ataatggcga caccacgacc gatgtgccag tcaccatttt gtggttttgg
     1081 tgaagaccat tggatcatct cgcgcccctg acgcaggatc tcttccagtg cgcagctggc
     1141 ggcggaagga acggaggtcg gcgctttacc ttcaccgatt gcaccgagaa ttttcagctc
     1201 ttgcccttcg tgtacccggt tacgttcgat aatttccagt tggtcgatct gtaactgttc
     1261 agccagttcc gctaatgcca tggtgatagc gaagttacct ttcggtgcgc cataaccctg
     1321 ataagcacca tttgggcaaa tgttgctgta gtaggtggtg acctggaaat cgacgttatc
     1381 gcacggatat aacggcagcg acagcgccgg tccgttacac ggtacggtga gtgagtggtt
     1441 gccgtaaggg ccagtgttgg cgcggaaatc catcttcact gccgtcaggc gaccatcttt
     1501 tttcgctccc agtttgacgg tgactttcgc gacgtgacga gaggtgttag caataaactc
     1561 ttcttcacgg gtgtagcgga acagtaccgg acgcccggtc acgcaggttg cccaggcgca
     1621 cacttcttcc agcaggatgt cctgtttgga accaaaaccg ccgccaactc gctctttaat
     1681 gacatgaact ttatgctgtt tcatgtccac gaggcgcgcg acctggcggc gtaagtgcca
     1741 tggtacctgg gtggaggcgt ggataaccag acgatcgccg tccatccggg taaagcagat
     1801 atgtgtttca gtcgggcact gctgcgcctg cgttgagtta taggttcgct caatgatcac
     1861 atcggcatcg gcaaagcctt tgtccatatc gccaatatga ccatgaatac tagcggcgat
     1921 atttttgcga gggcgagaac cgatcgggaa gttgatgatc atatgctcgc cgcgctgggc
     1981 tgcatggctg ttatcgtctt ccagagtatc tggcgcacca gcaacataca ccaccggttc
     2041 atcgtgcacg acaggcgcat cttccgccat tgcttcgtcg atcgacatta ccggcttaag
     2101 cacttcatat tcaacctcga tgagtttcaa tgcttcgagc gcaatttctt cactttcagc
     2161 gaccaccgca gcaacgcgat cgccgacgtg acgcattttt ttgccgaaca tacggcggtc
     2221 aagcggtgac ggttccggtg cgctctgacc ccccggggta tagtagatat cagggcaatt
     2281 caggtgagta ataacgtgaa cgacgcccgg taaggcttca gctttgctga catccagatg
     2341 agtaatcagt gcgtgagcgt gtgggctacg taacatttta atgacgcagg cgtcagccgt
     2401 tacgcggtct tcaacatagc agggtttcgc ctgcaccatt ttcgcggcat cagttttagg
     2461 ataatgcttg ccaatgactt caaggtcgtc acggaaagtc ggagcgatat caatggtggc
     2521 ctgcggatta tttttacgtg caaccgccag ttcaatgacc tgatagtatt gctgccagcc
     2581 agcatcgcgg ctgaacaaac cagaaagcgc gtcgtcgatc tcttcgcggg taggtgcggc
     2641 gatgcgatcg agaagatcgg tgataatcag agctgcagct ggatcgttat aaccagactg
     2701 caccacgcca acatcaacca ttgcctgttg aaccagactt aactcgttcc atttgcccag
     2761 agattctgcg gtacgaatat ctgccttctc taactgtgcg gcaataagca aggacgcgtt
     2821 aacgatatta ccgttaaaga tgattgcgtc agaaccggca aacccgaaac catcatcact
     2881 gttgcgtaca gagtgcattc ccatgttaaa caacagcttt tgcacgtttt cgcctggatt
     2941 aacggttagc tcctgaggcg cgccatttaa agtaaagtgg atgatcatac ggcttcctcc
     3001 ccagcctgtt ggcagtcggc atacaggtcg gctaccagaa ctcccgtgat atagcgttta
     3061 taggccacgc tgccccgcaa atcttcctgc gggaaaatgg cgttggcgac agcctgttca
     3121 agtgcattgc cttccagatt ttgtttttcg acatcatgca gacgcagtgc tttactggcg
     3181 acgccatcca gcgcaattcg cataccgtcg tggtctgtca ttgcaacggc tgccgtcacg
     3241 acggttaaac ctgcctgaga acggctaatt ttgcgggtcg cacaggtgcg atacggatct
     3301 ttaatgataa tttcggttag caggcgatcg catgggcagg ccaggtagtc ctcgattgac
     3361 agcgtttcgc cgttgccaaa aaccagttca gcatccagtg ccagcaggac gggaagcagc
     3421 actgactctt cctgccgggc ggcgatttca ccaccaatgg tcgactgatt acgaacatgg
     3481 cgtgagtaaa caaaaccgag ggcttcacgc agtgctgcag gaataaatcg cgcatcacgc
     3541 agtggctgca agcgagacat tgcgccaatc cgcagtgcac cgttatccca gtcaacccag
     3601 tccagttcca gatcctgtaa ggaaatggca atctttttat cggtacgggt tggtgtagca
     3661 ttgagtttgc tgcccccggc gaaccagacg gcttcatcct ggtagcggcg cttcagttcc
     3721 agcgcctgtt cgacagagtc gggcctgaaa aattgttcaa tcatcttctt tccttattta
     3781 accctcttcc tgcgggaaga gggcatctgt catttatgcc agcgcatcca tccgacgcca
     3841 catactggca gcggcttttc tggcttgtgc ataaatggaa tcgcaatcga agttaaactg
     3901 acggtcttca tagaccatca caccattgac catcacgctg tgaacgctgc ctgagcccat
     3961 accgaaagcg atatgcccgg cgatattgtc tgccagcagc ggcgtcggcg agttgtaatc
     4021 gcaaatggtt aaatcagctt tgtaaccggc ttccagaagc ccaaatttcg cgccaaagtt
     4081 gcggctcatc agttcgttac cgttagtcag ggctttggca aaactgtcag gccacagcgg
     4141 accacccgca tcgcgatgtt taaagaaggc aaatttcatc tcttcaaaca tgtccgaacc
     4201 aatgccgtcc gttcccaacg ccagattgcg gatgtcgcta aggtgatggt tgtagccgac
     4261 atggttgttc atgtttgaac gggcgttatg caccaggaac gcatcgcgct gattgagtag
     4321 ggcgatgtca tctttcgaca agtacagccc atgagcgacc agcgttttgc tatcgatgag
     4381 atcgaattgc gccagtcgtg ccagcaggtc tttgccgtac cagtggtgac tgtaggaaac
     4441 gtcgtaaagg tcttccgcag cgtgaatatg caaaccacgg cctgtggcgt tcacggcttc
     4501 acgtagcatc tccagaccgg catctggcac ggtaaacggc gcgtgagcac cgatatgtgc
     4561 ttccaccaga tacggctcgg ttgctgcttt cttcgcctca tcaatctgac gggcgaagcg
     4621 gatgttttct tctacacctt cctgcaactc tttgatgccg ttgttacggt cagtagtttc
     4681 aaaacaggtc atcgcgcgca ggccaacttt taaaaatgcg tcgcgcaatg tggagagcga
     4741 cccgccgata tacgccggag aggcatggtg atcgataacc gatgtacatc cgctcttaat
     4801 cgcttccagg gaacaaatca gcccgctgta atagagcgac tcttcatcaa gggcgcgatc
     4861 gagccgccac cagagatttt tcagcgttga gatgaaatct gggctggggg cgatgtttgc
     4921 cataattccg cgggaaagcc ccgagtaaaa atggttgtgc gagcagacaa ttcccggcat
     4981 cacaatccga ccatgcatct ctttgtagct ggcatcgggg tagcgttgcg tcagggcatc
     5041 gccgatagcg acaatcacat cattttcgat agcgatatca acgccttcct gcactttcgc
     5101 cgggtgtaac tgcactgcgg tgacattctt cagaatcaac atgattacac ctccacgcgg
     5161 cccagcagat aatgatgatg ctggtggaca tggctgatga tgcggcacat atcgttcagc
     5221 tccggtggta cgttgttgaa ctgaccttcg ctgtcgatgt ttaacaccca gctttggtta
     5281 ttcagtcgta ctcgtacccg gcaatcttcc acaaggaagc ctgggttgct gctgttatca
     5341 aagtcttgcg ccaggctgaa aacggtgatt ttgtctttgt atggtttacc gttccacgga
     5401 cagaactgag cgcagttgcc gcattcgtta cagtaagcgt cgaggtgcag cgtctggaat
     5461 ctattctgga agcctgggac cgcaatggag acgttggcgc ggttcgggca gacatccaca
     5521 cacttgctgc aaacgtagtt acattcaagg cagcgagcag cttcctgcgc gacaaacgca
     5581 tcacgatcgt cactgttcac cagcgtgata gagatatcgc ctttacgttg atagatttcc
     5641 gccggattga cgttgttcca gtatttatcg ttctggtggg aacggatatt ttcccgacta
     5701 aggatggcat cggtcgcccg acgcgcggtt ccgacagcag cgacaatgga ggatggtccg
     5761 cgctgtacgt cgccgatcat aaagacgtca gtcagacgag tttcgccatt atggtcgacg
     5821 tctggccagc cgtttttgtc cagcggcacg cccatcgcat tcagggcttc agtatcctgc
     5881 tgttcaccaa tggcggtgat caggctgtct acatgcagtg ttactgtttc gttggtttca
     5941 accggacgac gacgaccttt ctcatcgggt tcgccaagcg acataacgcg caaggttaag
     6001 gtgccatcag catcgaaacg ttccggatta ttcaggaaac ggaactccac gccgtcgtgc
     6061 aacgcttctt catactcttc gcgccatgcg ggcatctctt gcagtgaacg acggtaaacg
     6121 accgttgctt tttctacgcc tggaacgcgt aacgccgcac gagcgcagtc cattgcggtg
     6181 ttacccgccc cgacaacgac cacatgtttg cccagcttga gcgctgtacc cttgttgtat
     6241 tcacgcagga aggggagtga tttcaagaca ttttggttgt cgcccgccag tttcacaccg
     6301 ctatttttat ctgtgccggt ggcaatcaga acatagtgga agtcctgatt ttttaactgc
     6361 tcaacggtta aatcgggtga gcagccatac tcaaatttca cgccgtgagc ggcaacaaaa
     6421 tcgatatcgt gctgaattaa ctcagccgga attcggaact gaggaatgat atttttcacc
     6481 acgccgcccg cattggcttc gcgttcaaac agcgtaaccg gatggcccgc tctggcaagg
     6541 aagtaacctg ctgccagacc cgccggacct gcaccaatca cggcaaccgg atggcgtgaa
     6601 ccagaaccgg ctggtttgtg ccagcgttgc ttatattcat cccaaccttt ttccagcgcg
     6661 acttttttca gttcgcggat attcagcgca ctgtcgtaat ccaggcgggt acagttgtat
     6721 tggcactggt gatcgcaaat atgaccggta atggcgggca gagcgttgcg ttggtagatg
     6781 agttccagcg cgtcggcata gcggtgttcg ccaagcagac ggatgtattc cggaatatct
     6841 tgcttgatag cgcaggcagt aacacagggg gcaacgtagc agtcggtcag cggcaggtct
     6901 tctgccactt caatacgctc ttctggcttc cagtgtttct gggtgtattc catggttaac
     6961 gcatctgctg ccagtctgtt cagtcgttcg acgtcaacat ggtcaagtcc ccaggcgtcg
     7021 gagccttcca gctcgcgcat gcaggcactt aagcgcaaat agccgccagg tttcagcagg
     7081 tcggttgcca tagtaatagg gcgaatacct gtatcaaaaa tatcgcggat agtcagctga
     7141 ctggcaccac cggaataaga aatgggcagt ttgccgtcaa aggcgcgaga gagaactgct
     7201 gcaacattga tggagagcgg gaacagcgca cggcctgaca tatacatctc ttcaccaggc
     7261 agtgcgcctt tattgttgat ggtgccgaga gtgttagtca gttttacgcc aaagccgagt
     7321 gatttttctt ttgccagtgc catcaggcgt tccagcattt ccagtgcttg cgtcagcttg
     7381 aggtcgtgat caaatgactc ttcttttaag cctatgtaac cgaaaccgca gacatcgagg
     7441 atctcacgaa cacgcgcgta ccccagtaag gtcgggttaa gtttcacaaa ggtgttgagc
     7501 cctttttctt ccagcatgta gcggcaaatg gcttcgattt catgcggagg acagccgtgc
     7561 atggtggaga gggtgacgcc atgcaccata ctggtgggga tgcgagcggg taaggcttgc
     7621 aagctttcgc gtttttcctg caatccgtgg cgggctaaaa atgcgtcatc ctggagtaat
     7681 ttattcagcg tatcgcgata ttgcgcgaat ttcggatggt cagatgcgtc catcatattg
     7741 tcgatgaact gttgcattgg cggttgctta ataccttcga ggttgtaacc gacgctcata
     7801 ttaaagatga acgatttacc ggaatcagaa ggttggaaca tcgcttcgag aaggtgcagg
     7861 gcaaaccagg ctttgaggta ttcatcccag gctttaagca gggtaaattc ggtagaccat
     7921 tcggtgttaa agcactcgtc ttcggcatca atacagggct tttccagctc caggcggtca
     7981 agaatttgga cggtttttag ttcgatgaaa cgtccgccag tcagccagga agtgacaata
     8041 ttttgcgcga gctgcgtgtg cgggccagcg gcagggccga cgggagtggc acaggtttca
     8101 ccgaagacgc taacagtttt accttttaca ggtgagtaaa actgttgctc gggaatacca
     8161 aagattgagc gttgttgttg gtattcatca aatatgcgcg tcaaaagttc ctcaaacgga
     8221 atgggacgca taatatcccc cataaccctc tccttagctt atacagatat cattgagtga
     8281 ccggtgttga gaaatccggt ggtttagggg gatagagcaa caatcgcacc actcttgcca
     8341 ggtttggcgg gaaatataaa aaaatgtgag gcggctcgaa acttaagggc tttttgtgaa
     8401 tgaactcaca ggtgctaaat gagaattaaa atcactcctg aaaattatta ttgccatccc
     8461 atatatcact ttttctcttt tctcaaaatc agtgattcct tatcattatg ataaagagtg
     8521 ctggagggat taaatatttg aacgcaatcg tttaactacg ttttttaaat tttagtatac
     8581 cttttttgcg ctgtaataaa gtcgtccgga gtatcaatat ctaaaattat ttctgcattt
     8641 tctacttcga cggaataatg agctcccata aggagtgcct gacgcatatt ggtgatattg
     8701 gggcgcttga ttgcctgcat caggcatggt tttgacacta aaatcggatg gccggggatg
     8761 ccattatgga gtggcagtat tgcgccatca tttcgtaacg accagatttt tctgaaaata
     8821 tcgatggtga gggttggcat gtcaccgtgg gtgagaaaac aatgttctgt ttgcactgcg
     8881 ggtactgcgg cctttactga tgttagtaaa ccctgcgcat aatctgggtt gtggataata
     8941 gtgatattgc tctggttcgc ataacgttcg tgcagttcat taccacgata gccggtgact
     9001 aaaataattc ggctacaaaa ctgcaacgca tttttgatac ttgtatcaag aattgtcccc
     9061 tgttgccagg gtaacatcat tttccattgc cccattcttg atgataatcc ggcagcggta
     9121 attatacagt cgatggctga catagtggtt ccattattta taggggctaa taacgcctga
     9181 taattgcgtt acttcctgat ggattaagtt ggggataatt tatcccagag aggtcataaa
     9241 gactcgtgaa aagtatagtt gacccatcgg cattattcat tgatttaggt gcgcagaaac
     9301 gtcccactgt gatttctgtt gttggtgctg gggggaaaac cagcttgctt ttttggctgg
     9361 cagaactgct ccaggctagc ggcaggcgtg tgttaatcac gacgacaaca catatgttta
     9421 tgccaacgtc tcactggccc gtggttttct gtcgtgatcc cgccatgctt cctcatgcgt
     9481 ctcttacatc ccccatttca ttttgttttc acagctggaa agcgaacctg ggaaaagtgc
     9541 agggatttac gccagaagcg attgatgcac tggtgcaacg accagagtgt gacgtaattc
     9601 tcattgaagc agatggctcg cgtgcaatgc cgttaaaagc gcctgatgag cacgaacctt
     9661 gcatacccaa aagcagttgc tgcgtgattg ctgtgatggg aggacatatt ttgggcgcga
     9721 aagtgagcac ggaaaatgtc catcgctggt cgcagtttgc tgatattacc gggttaacac
     9781 ctgatgcaac cttgcaactg agcgatctcg ttgcgctggt tcgccaccct cagggggcgt
     9841 ttaaaaacgt accgcaaggt tgtcggcgag tctggttcat taaccgtttt tctcaatgtg
     9901 agaatgcgat tgcgcaaagc gagctccttc aaccgctgca acaacacgac gtagaggcaa
     9961 tctggctggg cgatatacaa gaacatcctg caatcgcgcg cagatttgtg aattagcggc
    10021 ccgggtattc cgggttactg aaaggatatc cattgaggtt ggtatgaata ttttcacaga
    10081 ggctgcaaaa ctcgaagagc aaaattgtcc gtttgctatg gcgcaaatta ttgatagccg
    10141 aggctcgact ccccgccatt ctgcacaaat gttagtgcgc gccgatggct ctatcgtcgg
    10201 tacaattggt ggcggaatgg ttgagcggaa ggtgattgaa gagtcgcttc aggcattgca
    10261 ggaacgtaag ccgcgattat tccatggacg tatggctcgt aacggtgcgg atgctgtcgg
    10321 gtcagattgt ggaggtgcga tgtcagtgtt tatcagcgtc catggtatgc gtccacgtct
    10381 ggtgttgatt ggcgcggggc atgtcaaccg ggcgatagcc cagagtgcgg cgctattagg
    10441 atttgatatt gccgttgccg atatttatcg cgaaagcctc aatcctgaac tattccctcc
    10501 atcaaccacg cttctccatg ctgagtcgtt tggtgcggca gtggaagcac tggatattcg
    10561 ccctgataat tttgtcctga ttgccacgaa taatcaggat cgtgaagccc tcgacaaact
    10621 cattgaacag cccattgcat ggttggggtt gctggcaagt cgtcgcaagg ttcagctttt
    10681 cctgcgtcaa ttgcgtgaga aaggcgtggc tgaagaacat attgcccgtt tacatgcgcc
    10741 cgttggttac aacataggtg cggaaacgcc gcaggagatc gccatcagcg tgctggcaga
    10801 aatattacag gtgaaaaata acgcgccggg tgggctgatg atgaaacctt ctcatccttc
    10861 cggacaccag ctggtggtga ttcgcggtgc gggagatatc gccagtggtg tggcgctacg
    10921 tctgtatcat gcgggtttta aagtgatcat gctggaagtg gaaaaaccga cagtgattcg
    10981 ttgtaccgtg gcgtttgccc aggccgtgtt cgatggcgaa atgacggtcg aaggcgtcac
    11041 tgctcgcctg gcaaccagct ctgcggaagc gatgaaactt accgaacgcg gattcatccc
    11101 tgtgatggta gatcccgcct gttcattgct tgatgaactg aaaccgcttt gcgtggtgga
    11161 cgctattctg gcgaaacaga atttgggaac acgggcagat atggcaccag taacaatcgc
    11221 gcttgggccg ggctttgctg cagggaagga ttgtcatgcg gtaattgaaa caaatcgcgg
    11281 gcactggctc ggtcaggtga tttactgtgg ttgtgcgcag gagaataccg gtgttcctgg
    11341 caatattatg gggcatacca cccgacgggt gatccgtgct cctgctgcag gcattatgcg
    11401 atccaacgtg aaattaggcg atctggtgaa agagggcgat gtgattgcct ggattggtga
    11461 gcatgaaatt aaagcaccgt tgacggggat ggtgcgtggc ttgttgaacg acggcctggc
    11521 agtggttggt ggttttaaaa ttggtgatat cgatcctcgt ggtgaaaccg ctgatttcac
    11581 cagcgtttct gataaagccc gggcgattgg cggcggcgta cttgaggcgt taatgatgtt
    11641 gatgcatcag ggcgtgaaag cgacaaaaga agtgctggaa gtggcttaaa tgaaaatggc
    11701 cggatggtat tacccacccg gccttaaaat tactgacaaa tcaccgttcc ggttttgcct
    11761 tcaatccctt ctttcgcttt gctcaatacg gtaatcagtg cttcacgacc tgcgcgagag
    11821 cgagcaaacg aggcggcagc ttccacttta ggcagcatag aacctttcgc aaaatgacct
    11881 tcttcgataa agcgttcagc atcacttaat gacaggcgat cgagccactg ttcattctct
    11941 ttaccgaagt taatggccac tttttctacc gcagtcagaa tgatcagcat atcggcatca
    12001 atcatttctg ctaaacgggc gctggcccag tctttatcga taaccgcgct ggcaccacgc
    12061 agatggttac cttcacgaat aacaggaata ccaccaccgc caacggtgat caccacctgg
    12121 cctgcttcta ccagcgcttt taccgtctct ttttcaatga tatcaaccgg ttttggcgag
    12181 gcaacaacac ggcgatagcc gcgaccagca tcttctttca gagtgtaacc ctgttttgtc
    12241 agttgctctg cttcttgctc ggtaaagaac gaaccgatcg gcttggtcgg gttgaggaat
    12301 gccggatcgt tagcgtcgac ttcgacctga gtaaccagcg ttactacagg tttattaatg
    12361 ccccgagaaa gcagttcttc tcttaaggcg ttttgtagat cgtaaccaat ataaccctgg
    12421 cttaaggcaa cacagacaga catcggcagc attggcgaat gcgcttcagt ttttgcggca
    12481 gcttcaaagg cctggttaat catgcccacc tgcggcccgt taccatgagt gacgataact
    12541 tcatgtccct gggcaattaa atcaacaatt gcctgagaag taatttttac cgctttcatt
    12601 tgtccggcca ggtcatcgcc cagcgcattc ccgcccaggg cgagaacaat tttcttactc
    12661 atttttcctc actcatcatt cttgattgtg tatagatccc tggcagtggt tagagcacgg
    12721 gagggacaaa cggtttgcgt cgcaggaaac gacctcgccc ggctttgcct gtaaaggtgc
    12781 cgtcacagaa aatcgtttca ccacgggata aggttctgac aatcgcgccc tgacaggtaa
    12841 aaccctccca tggcgagtag tcggcgttgt cgtggagatg gcgatgctga atttgttggc
    12901 tctgacgtgg gtcgataatc accacgtcgc catcggaacc gggcgctaat aatccttttt
    12961 gcggccacag gccaaacagc ctggcgggca ttgcgctggt taattcaaca aagcgttccg
    13021 gtgttatacg tcccgtcatc acgccactgg aaaacagtaa ctgcatgcgg ttttccacac
    13081 cgggtaagcc atttgggcag cgactgaaat cgcctttaga aatttgcagg cgttgagcca
    13141 tcgagaaggt gcagtgatcg gtcgccacca cgtcaatcgc accatcgctg atgccacacc
    13201 acagtttgtc ctgctcgcgt acgttacgca gcggtgggct aagaatgaac ttcatgccgt
    13261 cttctgtatc gtaactgcgt tcgtccaaca ggagatattg tgggcaggtt tcaacccaga
    13321 ctggctggtg attcgcacgg gcaagacgca gataatccag acctaagccg ttagacaggt
    13381 gcacgatata gagcggggcg ttaccggcaa tttgtgccag gttaatcatg cgggcgatgg
    13441 cttccgcttc gcattccaga gggcgactca aggcatgata gcgcggcgcg gttaaccctg
    13501 cggcgataaa ttccgcccgc ttgctggcga tagccgcatc attttccggg tgcacggtgg
    13561 tcagcgcgcc ggattcatgc agacggcgta atgcctgcaa aacctcgtca tcgttgagtt
    13621 tgtattgata ggttaaatag agtttaaaac tgctcagccc ttcctcgacc atcatcggga
    13681 tttcgtcgag gatggcgtga ttaatgtgct ggatcacacc gtgaaagctg taatcgatga
    13741 ccgctttatg ggcggcataa ccacgataaa cctccagttg atggcgtaac cgacagccgt
    13801 ttgggccaaa tcccatatgg tcaataatgg ttgttgtacc gccacacgca gctgcgcggg
    13861 taccggtaaa aaaatcatca caactgcgcg cgatgccgac atcaatattg aaatgcgtat
    13921 ggacatccac gccgccaggg aaaacgtaac agccagtggc atcaatttct tcatacggga
    13981 gctgcggcga aatattgttg cccaactggc gaacaatccc gctttcaatc agcaaatcct
    14041 gtttggcttg tccatctgcg ttaacgacag tgccgttttt gatcaatacg cgcatagcaa
    14101 actccaaaaa ccctccggcg aaccggaggg gaataagtga agagtaatta ttcggttgcc
    14161 agccagctta acgggattgc agcgtacatc gcggcacagg tcaccaggtg agatttccag
    14221 gttttttcgt taggtgcatg cgcttcaggt tctttacccg ggccaaagcc gatcaccgga
    14281 atgccgtgac ggcccatgat agatacgccg ttagttgaga aggtccactt atcaacaacc
    14341 ggcgctttgc caaacagccc ttcgtaggca ttcaccagtg ctttaacggt gaagtgatct
    14401 tcttccactt tccaggtcgg gaagtagcat tcggttgggt aaaccaggcc agtccaggac
    14461 ggacggtcgt agttgtacat agaaacaacc gcgttagctt tctgtactgc aggcagggcg
    14521 cggatttcgt ccagcgcgcc ttcccaggtt tcgccccagg tcagacggcg gtcaatagag
    14581 acggcgcagc tatctgctac agcgcaacgg cttggggatg tgaagaagat ttcagaaacg
    14641 gtgagggtgc ctttgccgag gaattcgtca taacccagac gttgggagag ttcttgtaat
    14701 tcgccaagaa tcggacccat tttgaaaatg gcgttgtcac cgcgttctgg cgcagaaccg
    14761 tggcagctaa caccctgaac atcaatacga atttccatac gaccgcgctg accacggtat
    14821 acctggcagt cggttggttc ggtactgacc acaaattccg ggcgaatgcc ggattgttca
    14881 ataatgtact gccagcacag accgtcgcag tcttcttcct gcacagtacc ggtaaccagc
    14941 agggtatatt catcttccag accgaggtct ttaatgattt taccggcata aaccatagat
    15001 gccatgccgc cttcctggtc ggaagtaccg cgaccaccga tcagctcatc agtttccatg
    15061 ccttcgtacg gatcgaagtc ccagtttttg atgttgccaa tgccgacggt atcgatatga
    15121 gcgtccattg ccaccagacg cgggccgtgg ccgatataac cgagaacgtt acccatcggg
    15181 tcgatttcaa ctttatcgaa gccgactttt tccatctctt ctttaatacg atgtactacc
    15241 cgtttctcgt cgcagctttc actgggaata gcaaccatgt cgcgcaggaa gcgagtcata
    15301 tccgcctggt aatcttttgc tttttcaaga atcagtttga atggaatatt cttagccatt
    15361 atttttctcc agatttttta ctccccgttt tgcagggagc agttccaata gggtgattaa
    15421 ggtgctacag cgtgtttgcc ttcccagaca acttcgcggt agtgcttcac gtcggtgtcg
    15481 ccttcagtgc tgataaccag cactacggca tctttgttca gcgccagttt ttccatcagg
    15541 ctttgacgtt gcgggtgata atgaaccgct gcgagaacgc ccaaaccgac agcgccggat
    15601 tcaccggaga tgatgcgcgg gtcgttgccg tacggattac ccagcacgcg catacctaat
    15661 gcggcaacgc tgtcctggca ggagatgaat tgggtggcgc agttacgtag gatttcccag
    15721 cccagcgggt taggttcgcc acaggccagg cctgccatga tggtggccat atcaccgcca
    15781 acgttgacga tgtcgccttt gacgccggag cgataaatac agtcagcttt gtcaggttca
    15841 acaataatgc tgtgcagatt ttgcgggcta tagacgtcga ccagataacc cagcacacca
    15901 ccggccattg ctccgacacc ggcttgcagc agaacgtgcg tcggggttac gcccatttca
    15961 cgcatttgct cgacggcttc atctgccagg gttgcgtagc cttgcatgat ccaggttggg
    16021 attttggtgt aaccttccca tgccgtgtcc tgtaccactt cccagccgtg ctgctgcgcg
    16081 tgttgcatgg tcaggcgaac ggtatcgtca tagttcatat ctgtgacgat gcactcggca
    16141 ccgaggttca gaatggcgtc aacgcgttcc tgagcagaac ctttcggcat gtaaatcacc
    16201 gcattctgtc cgagttgctg cgctgcccac gccacaccgc gcccgtggtt gccgtcggtg
    16261 gtggtcgcga aagtcatttt ttcgccgatg gcatttttca ggtgctcaaa tgacagcgtt
    16321 tcgatatcaa gatgatattt ttcacacaat aattgagcga tagcgtacgc accgccgagc
    16381 attttgaagg cgttcagacc gaatcgtttt gattcgtctt tgacaagaat tttcttcacc
    16441 ccaaaaaggt ttgcgagatc gtccagcgca caaagcggtg ttgggcgata accagctatt
    16501 ttctggtgga actggcgcgc cagtttggcc tggctttgcg aaaagagcgg tgatgtttcg
    16561 ccgttgaaaa atttgttatc ggcgatatca atcttcaatg agaaaacgga catatcctat
    16621 ccttttggcc tctggaaata gagggccgac cagctgatcg gcccgagggg ttatttcacg
    16681 cgttcttgcg cttctttcag cagttgctcg agcagtgcac ctggtttggc gtatttacgg
    16741 gacaggatca tcgcggcgat gatataaggc ttccagctgg cttctttgta ggtagcgata
    16801 cggtattttt cgaatacgcc ttcagtcact tccccttctt tgcaggatac gccgctgata
    16861 tcagctggca ggcagtgcat gtacagggct tcgccatcac gggtcagttc catcatctct
    16921 tcagtacaat gccagtcttt gtgttgcgcg ttctgtgcca gacactgttt ttccagtgct
    16981 tttaagcctt cgtgatcgtt agcgcgcagc aattcggtac gctgttccat cactttgtaa
    17041 ggtgcccatg acttcggata aacgatgtct gcatctttga acgcttcttc catgctggtg
    17101 acctgacgga agctaccgcc agatgcttta gcattgtttt tcgcgacttc tactacatcc
    17161 gggatcaggt cgtagccttc cggatgggcc agggtgacat ccataccgaa gcgagtcatc
    17221 agaccgatga tgccttgtgg tacagagagc ggtttgccat agcttggaga gtaggcccag
    17281 gtcatggcga tttttttacc tttcaggttt tccagtgaac caaagtgttc acgtaaccat
    17341 gccaggtcag ccattgactg agtcgggtgg tcaatatcgc attgcaggtt cactaaagcc
    17401 ggacgctgtg gcagtacacc ctgcttgtaa ccgtcatcaa gtgcagcgcc aacttcacgc
    17461 atataggcgt tgcctgcgcc cagatacata tcgtcgcgaa taccaatagc gtcggcgcag
    17521 aaggagatca tattggcggt ttcacgcacg gtttcgccgt gagcgatttg tgatttgcct
    17581 tcatcgagat cttgctgtgc aaggccgagc aggttaagcg cggaagcata agagaagcgg
    17641 gtacgagtgg agttgtcgcg gaatacggaa atacctaatc cactattaaa gactttggtt
    17701 gagatgtttt cagcacgcag tgcttttaat gctgcggcaa cgtccagtac ttgtttcagt
    17761 tcatctggag tctgttccca cgttaacaaa aaatctttct cgtgaaggtg agaggtcagc
    17821 gaattgatat ccttaatcag ctcattaaca gttttcatca taaagtcccg gtagtggaat
    17881 gacaggcaaa gtggtttatt tcactgtgac ttgccaaagt gattggtgtt ttcacagcta
    17941 tacctgggac taacaataag tgtgccagaa ttatccacgg ggctgcaaaa gactaatttg
    18001 acggataatg tgtggggttg atcacaaatt gatgccagct tccggttgtt tgtaaaatac
    18061 gaaaacgttt ggcttttttt atcgcgttat ttatttgctg ctaagtatca gaaataagat
    18121 aaagggacag gctatccctc gtcagtttga taaactcacc gccataattc tcatattgat
    18181 atagaaatgt gatatctctt ccataatttc attagaaagt gataaaaatc ttatgttttt
    18241 atcaaagcca atttcgcctg agttaatatg atcatgatct gtgaaccatc aacgtcttca
    18301 atgatcgact tttaagtaag tctggaaggt aaagctatgg agcttgctac tacgcagtca
    18361 gtattgatgc aaattcaacc gacaattcag cgttttgcca gaatgcttgc cagcgttttg
    18421 cagcttgagg ttgagatcgt tgatgaaaac ttgtatcgcg tcgccggaac gggcgcgtat
    18481 gggaagtttc ttggtcgcca gttgagcggc aactcacgcc tgctctgcca cgtcctggaa
    18541 acgaaaactg aaaaagttgt gacacagtct cgcttcgatc ccctttgcga aggttgcgat
    18601 agtaaagaaa attgccgcga aaaagcattt ctgggtacgc ctgtcatttt acaggatcgt
    18661 tgtgttgggg tgataagttt gattgccgtt acccacgagc aacaagagca tatcagtgat
    18721 aatttacgcg aattttctga ttatgttcgc catatatcca ccatttttgt ttcgaaactt
    18781 ctggaggatc aggggccagg agataacatc agtaaaatat tcgcgaccat gatcgataat
    18841 atggatcagg gcgtattagt tgttgatgat gaaagtcggg ttcagtttgt taatcagact
    18901 gccttaaaaa cacttggtgt tgtgcaaaat aatattattg ggaaacctat ccgtttcaga
    18961 ccattaacat ttgagagtaa ttttacccat ggacatatgc agcatattgt ttcgtgggac
    19021 gataaaagtg aattaatcat tggtcaattg cataacattc agggccgaca attattttta
    19081 atggcgtttc accaatcgca taccagtttt tctgtagcaa atgcacctga tgaaccgcat
    19141 attgaacaat tggttggcga gtgccgtgtt atgcggcaat taaaacgact cattagccgt
    19201 attgcaccca gcccatccag cgttatggtg gttggtgaaa gcggcacggg taaagaagtt
    19261 gtcgcccgtg caatccataa gttgagcgga agacggaata aaccctttat tgctatcaac
    19321 tgtgccgcga ttccggagca gcttctggag agcgaactgt tcggttatgt taaaggcgca
    19381 tttactggcg cttctgccaa cggtaaaaca gggttgattc aggcggcgaa tacgggcacg
    19441 ctgtttctcg atgaaatagg tgatatgcca ttaatgttgc aggctaaatt actgcgcgct
    19501 attgaggcac gtgaaattct gccgattggt gccagtagcc caatacaagt cgacattcgc
    19561 atcatttctg caactaacca gaatttggcc cagttcattg ccgaaggtaa attccgcgaa
    19621 gatctcttct accgacttaa tgttatcccg ataactctgc caccgctgcg tgaacgtcag
    19681 gaagatattg aactactggt gcattacttt ttacatctgc atacccgtcg tctgggatcg
    19741 gtttatcctg gcattgctcc cgatgtcgtc gaaatattgc gtaagcatcg ttggcccgga
    19801 aacctgcgcg agttaagcaa tttgatggaa tatctggtta acgttgttcc ttcaggtgaa
    19861 gttatcgaca gcacgctatt gccgccaaat ctgctgaata atggcacaac ggagcaaagt
    19921 gatgtaacag aggttactga ggcacacctg tcactcgatg atgcgggcgg cacggcgctg
    19981 gaggagatgg aaaagcaaat gatccgcgag gcgctttcac gtcataacag caagaagcaa
    20041 gttgctgatg aactgggcat cggcattgct acgctctatc gcaagattaa gaaatatgag
    20101 ttgttaaaca cataacatca gtgaatccgg cctgaattca ggccggatat ccttttactt
    20161 cgttttctcg caatccagaa ctgtatttac aatcatctga taccccgtgc agcgacaaag
    20221 atttcccgcc agtccgcgac gaatttccgt aatggttaat ggcttctcgc gtggtttcgc
    20281 cagcattgcc gtggtagcca taatcaggcc aggcgtacaa aacccgcact gcactgcgcc
    20341 ggatttcgca taagcctgct gaacatgaga aagttttccg cctttcgctt caccttccag
    20401 cgtgcggatc tcttttcctt cagcccaggc ggcaaggtat aagcaactgt ctattgctgt
    20461 gccgtcgacc agcaccgtac aggcaccaca ttcacccacg cagcaccctt gtttgacact
    20521 tagcagccct tgttcgcgga gtaattccga gagcggcgtg cctggtacgg catgaagctg
    20581 aaaaggcatc ccgttaatgg tgcattcgat ggtaattgtt tcgctgtgat tcattgcaat
    20641 tttccccccg ccgcggcgac ggcttcgcta atcacttttt tggtcatcgt ctggatgaga
    20701 tgcagacgaa actctttact ggcccgccat gaagaacgcg gggcgacatc ttgcaggaca
    20761 gattcgctga tagcttccag cgtttgcagg tttaatggcg cattttgtgc agtctgttcg
    20821 gcatgttggc agcgaatcgg cgttggcgcg gcaacaccaa atgccaggcg taattcgctg
    20881 aaattgccgt tatccagtcg gcaatgtgcg gcacagccaa tcgttgaaat atccattgcg
    20941 tcgcgcatgg catatttaaa atgcgcgctg cccacgtgtt ctttcggctg gggcggaaaa
    21001 tgaaaggcga caaggatttc gtcatgctca agagacactt tgcccggccc ggtgtgaaag
    21061 ccattaatcg ggacgaaacg aacaccgcgt ggggagtgga tctccagttt cgcgtcataa
    21121 atgagcgttg gcgtggcaga atctgcgctg gtggcaccgt tgcaaatatt tccaccgtag
    21181 gtagcgacgt tacggatctg cggtccagca atggacgagg ccgcagcaca taacgccggg
    21241 agatgacgtt gagttataga atcttctatt agctgggtaa atgtcgttgc agagccgata
    21301 cgtagcgagc catcttccgc cagcgtaatt ccccgcagct ccgccagatt atgaatatca
    21361 acaatatggc gataacggtc attgtgatgg tggagctgaa tcagtacgtc agtgccaccg
    21421 gcgagcagtt tggcctgcgg gttgtcagcc agcaggttga tggcatcggc aagggttgct
    21481 gcgcgatggt aagaagcaaa atcaaacatg atgttatcct caaatcaatc ctgccagatg
    21541 gaactcttca tataaccgtt ttggcgtcag cggcagtgta ttgattgcaa caccggtagc
    21601 catcttcacc gcgttacgaa tagcagcggc aacaggaatt attggtggct cacccagtga
    21661 cttatgtccg tatgcggatt gcggctcatt gatttcgacg aacgcgcttt ccagttgtgg
    21721 cagatccggc atggtcggca ttttgtaatc cagcagattg gggttacgga ccacgccgct
    21781 tttcgcatcg atgatcatct cttcaaatag cgcccagcca atgcccattc ccattccgcc
    21841 gtgtacctga ccttctgcca gtagcggatt aagaatatgg cccgaatcat gaacgttgag
    21901 gatgcggttg atggtgactt tgcacagcgc aatatcgacc gtcagatcaa caaaggtaca
    21961 gccgaacgcc ggtgggttag tggtggtttt gatggagctt tcagcagaga gctgcccgcc
    22021 gcgttcaggg tggtagaaag cgtccatcgc caaatctttt aacgacatta acggctcttc
    22081 cggtcgttca accagcacga tatggccttt tatcagggtc agattcatcg ctgactgatg
    22141 tagcatgact gcggcgtgag cgatgatttt ctcttttaat aacagtgccg cactgcgcag
    22201 cgcaggcgcg gcaacatagc tctggcgtga ggcaaatgcg ccgggatcga acggcgtaac
    22261 gtcggtatct tgtgttgaaa taacgcgaac gtcgctgacc ggaaccccca cggtttctgc
    22321 caccatttgc gagaagacgg tgtcggcacc ctgaccgatt tccgtcgcgc cgctttgcac
    22381 gttgatggtt ccatcctgat tcatcagaag gcgcgcgcct gctatttcta cgccgacagg
    22441 ccaggtgtta gaggtgtagc taaaacaggc gacgccaacg ccgcggcgta aattgccttg
    22501 ctggttctgg cactctgcac ggcgtttttc ccattcaaag attttccggc ctttttcaag
    22561 acactccggc aaccctgcgc tgtaaatacg tttgcccgtg agcggattag catctccttc
    22621 gcgtgaggcg ttgcgtaaac gaatttcaac aggatcaata cctaacgctg tcgcggcgtc
    22681 atcaagcata gactcaacgg caaatacgac ttgtggcgcg ccataaccac gcatcgcacc
    22741 agccgagggg aggttggtat agcaggtctt tgaactgtaa gcgtaggcac aacgaggata
    22801 aaggtaagcg actttatttc cccctgcaga agcgatggag tgcccgtgag atacataagc
    22861 gccggtgtta gacagaacat ccagactata acctttcaat gttccgtcgc ggttcacgcc
    22921 catttgcccg tcaatagtaa aagcgtggcg ggtacgggtt gcgaggaaac actcttcacg
    22981 gctaagggaa actttcaccg gaatgccgcc aagcttgctg gtcaggaatg ccgccattgg
    23041 ctcttccagt acatcctgtt tattaccaaa accgccaccg acaaatggtt tgatgactcg
    23101 tacgcatgac cagggaatat ccagcgcctg accaaccacg cggcgaacaa tgtgcgggat
    23161 ctgggtgctg gaaacgatgg taattcgcga gtcatcctcc atccacgcca gcgatgttac
    23221 gctttccatg tgacaatgtt gaataacggg ggtctgatag tgcccctgta cctggtagtc
    23281 ggcggcatcg attgtttgtt ggacattgcc cgtcgacatc gtgctttgtt tcagtaaatt
    23341 gccgccgtta tggattggtg cagcgtcctc tgccagcgcc gcttctggcg tggtgataac
    23401 gggtaattct tcccactcaa tgctgaccaa ttgcgccgct ttttctgccg tgagttcatc
    23461 gcgggccacg acgatggcaa cggcgtcacc atgatgacga acatggcgag ttagcagtgc
    23521 gcgatcggcg gtatcgcgct tgttttcgtc aagtgtccat gcatgcccag ctgtagcgaa
    23581 tgggatatca ggcacatctt cccaggtaaa aatcgccagt acgcctggta aacttctggc
    23641 ttgttcatca ttaatactta cggcataacc atgtgcgata gggctacgta catatttcgc
    23701 ataacacatg cccgccataa cataatcgtc agtatatcgt gcccgcccgg tgaccttagc
    23761 aatggcatca acgcgcatgc atgattcacc cgtagcggtt gcttcccgcg cttccataga
    23821 aatcccctcg atttgaacat ttggttctta tggcaaagat ttagtctaag caggcaagat
    23881 ttacgccaga aacggcgata agcgttaagg gaggaaggaa gtgtgatgat gttaaaaaaa
    23941 cacagatatg atacgaaaca ggaccctgaa taggggtagt gatacgtatc aatatgagtg
    24001 gcgataaaat caatgtgata aattaggatt attacgcatc aggatgggaa atagggtaaa
    24061 aaagtcagga acttcgatac ctctacagtg aatattttgc tgatgatact tccctttgcc
    24121 aaatgaaatt aacctctttc tctattacat cgtttttcca gaagcaagag attgggtatc
    24181 aagaggctgg ctgctatgat atggcgtctt gttttttaag cgaggaaaga ttttgagtgc
    24241 gggacgcctg aataaaaaat ctctgggtat cgtgatgttg ttatcggttg gactgctttt
    24301 ggcgggctgt tcgggtagca aatcatccga tacaggaacg tattccggct ccgtttacac
    24361 cgtgaaacgg ggggatacgc tatatcgtat ttcgcgcacc acgggaacca gcgtaaaaga
    24421 gctggcgcga ctgaacggca tttccccccc ttacaccatt gaagttggtc agaaactaaa
    24481 actgggtggg gcgaaaagta gcagtagtac acgtaaatca accgccaaat caacgaccaa
    24541 aaccgcatcg gttacaccgt catcagcggt accgaaatct tcctggccgc cagtagggca
    24601 acgttgttgg ttatggccaa cgacagggaa agttatcatg ccgtattcga cagcagatgg
    24661 cggcaataaa gggattgata tctcagctcc acggggtaca cctatttacg ccgcgggtgc
    24721 aggaaaggtg gtgtatgtgg gcaaccagct gcgtggctac ggtaatctca tcatgattaa
    24781 acacagtgaa gattacatta cggcttacgc ccataatgac acgatgctgg taaataatgg
    24841 gcaaagcgtg aaggctgggc aaaaaatcgc caccatgggg agcacggatg cggcatctgt
    24901 tcgcctgcat ttccagattc gttaccgtgc aacggcaatt gatccgctac gttacttgcc
    24961 gcctcagggc agcaagccaa aatgctgatg gcgaattaat cagcagtcag cagcgcggca
    25021 cttgctaaga agagcgtaag gtttataatg ccttacgcat ctcgaagcgg gcgtagttca
    25081 atggtagaac gagagcttcc caagctctat acgagggttc gattcccttc gcccgctcca
    25141 atttatcttc acctcatcaa tctgccattt ttgctgtatc tgattttcta tattatctta
    25201 attcaatcgc ttcagacttt tcgcaatctc tgtaacactc ccccattttt ttgtaaataa
    25261 aaagcgttta cgcaatgcaa tccttttgta acaacccagg taaaatacct tattttcaag
    25321 ttgttaaata agtggttatc tacgctttta acaaacgatt gatggaatat tttatgaaag
    25381 ggaaatctgc attaactctt ctgctcgctg gtatattttc gtgcggcaca tgccaggcga
    25441 caggggcaga agtgacgagt gaatctgtat ttaacattct taattccacg ggggcagcaa
    25501 cagataaaag ttatttatcg ttaaatccag ataagtatcc taattatcgt cttctgattc
    25561 actcagccaa attacaaaat gaaataaaga gccactatac caaagacgaa attcaaggat
    25621 tgctaacatt aacagaaaac acacgaaagt taacgttgac ggagaaaccc tggggaacgt
    25681 ttattcttgc ttctactttc gaagatgaca aaacagcggc agaaacccac tatgatgctg
    25741 tctggctgag ggatagttta tggggctata tggcattagt atcagaccag ggaaatagtg
    25801 tagccgcaaa aaaggttctg ctcaccctgt gggattatat gtctacgccc gaccagatta
    25861 agcggatgca ggacatcatt agcaacccaa aacgactgga tggtattccc gggcaaatga
    25921 atgctgtgca tatttgacag caactccccc gtgatggcag atgtgcagga agaaggcaag
    25981 ccgcagctgt ggaaccataa acaaaatgat gcgttaggtc tttatcttga tctccttata
    26041 caagcaatca atactggtac tattaacgct gaagattggc aaaaaggtga ccgactaaaa
    26101 tcggttgcgc tgctgattgc ttatctggat aaagccaatt tctatgttat ggaagattcc
    26161 ggagcgtggg aggaagatgc ccgattaaac acatcttccg ttgcactggt gacctctgga
    26221 ctggaacgtt tgtcaaacct actctcgaag aaagactctg tttttgtctc tgatttacta
    26281 cgcgaagcta aagtcaatga actggatgaa actctttcaa caacgaggtt gaaccacctc
    26341 atagataaag gttatgaaag aataacgtta cagcttgatt tagggggaga atccccaggt
    26401 tatctggaaa aagataaaca ttacagagaa gccgatgcgg ctttacttaa tgttatttat
    26461 cctgcaaatc tttcaaagat aaacaccaga agaaaagagc aagttctaaa aatagtta
//
LOCUS       DBFIFM010000046        26239 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.046, whole genome shotgun sequence.
ACCESSION   DBFIFM010000046 DBFIFM010000000
VERSION     DBFIFM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.046"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(98..2059)
                     /gene="topB"
                     /locus_tag="N7C74_001686"
     CDS             complement(98..2059)
                     /gene="topB"
                     /locus_tag="N7C74_001686"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HGU2431568.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRYLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLISRQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLIKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(2064..3107)
                     /gene="selD"
                     /locus_tag="N7C74_001687"
     CDS             complement(2064..3107)
                     /gene="selD"
                     /locus_tag="N7C74_001687"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HGU2431569.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(3224..3775)
                     /gene="ydjA"
                     /locus_tag="N7C74_001688"
     CDS             complement(3224..3775)
                     /gene="ydjA"
                     /locus_tag="N7C74_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HGU2431570.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            3936..5792
                     /gene="sppA"
                     /locus_tag="N7C74_001689"
     CDS             3936..5792
                     /gene="sppA"
                     /locus_tag="N7C74_001689"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HGU2431571.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKADKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKAKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            5908..6618
                     /locus_tag="N7C74_001690"
     CDS             5908..6618
                     /locus_tag="N7C74_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HGU2431572.1"
                     /translation="MNKAPSLIAAIVLGLGISACGYFVGDGVKHLKTNNRYVNVRGLS
                     EKEVRADTAELTIAINFKGNVPGELFPKLEEAQKKIVAELNAQGINEKEIILGQWTSK
                     RTDSFYLKDDPTMPRYNADGSVTIKTHNVAAVEKVVAKLNELQVATDGAIAESKVAYR
                     FNGIGALRAEMIAAATKDARNAALQFATDSGSQVGSISDASQGVFQIFASGSDEDDPT
                     AINKTVRVVTTVTYALQD"
     gene            6750..7766
                     /gene="ansA"
                     /locus_tag="N7C74_001691"
     CDS             6750..7766
                     /gene="ansA"
                     /locus_tag="N7C74_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HGU2431573.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            7777..8418
                     /gene="pncA"
                     /locus_tag="N7C74_001692"
     CDS             7777..8418
                     /gene="pncA"
                     /locus_tag="N7C74_001692"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HGU2431574.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSALFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(8511..9869)
                     /gene="ydjE"
                     /locus_tag="N7C74_001693"
     CDS             complement(8511..9869)
                     /gene="ydjE"
                     /locus_tag="N7C74_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2431575.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAECQLR
                     EVEQQIEREKSIRLPPLTSYQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSTLLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLLLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(9987..10745)
                     /gene="ydjF"
                     /locus_tag="N7C74_001694"
     CDS             complement(9987..10745)
                     /gene="ydjF"
                     /locus_tag="N7C74_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGU2431576.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(10882..11862)
                     /gene="ydjG"
                     /locus_tag="N7C74_001695"
     CDS             complement(10882..11862)
                     /gene="ydjG"
                     /locus_tag="N7C74_001695"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HGU2431577.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGFDYIDIYMTHWQSVPPFYTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEVLGR"
     gene            complement(11872..12804)
                     /gene="ydjH"
                     /locus_tag="N7C74_001696"
     CDS             complement(11872..12804)
                     /gene="ydjH"
                     /locus_tag="N7C74_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGU2431578.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVNIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMNICADMIKPRLNETLDDICEALSYVNYAEAKLLTGKETLDEIADSFLACGVKT
                     VVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNLRECAR
                     FANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            complement(12809..13645)
                     /gene="ydjI"
                     /locus_tag="N7C74_001697"
     CDS             complement(12809..13645)
                     /gene="ydjI"
                     /locus_tag="N7C74_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HGU2431579.1"
                     /translation="MLADIRYWENDATNKRYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            complement(13666..14709)
                     /gene="ydjJ"
                     /locus_tag="N7C74_001698"
     CDS             complement(13666..14709)
                     /gene="ydjJ"
                     /locus_tag="N7C74_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HGU2431580.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGTLVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGASAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            complement(14726..16105)
                     /gene="ydjK"
                     /locus_tag="N7C74_001699"
     CDS             complement(14726..16105)
                     /gene="ydjK"
                     /locus_tag="N7C74_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2431581.1"
                     /translation="MDQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRHTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGTPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            complement(16132..17208)
                     /gene="ydjL"
                     /locus_tag="N7C74_001700"
     CDS             complement(16132..17208)
                     /gene="ydjL"
                     /locus_tag="N7C74_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGU2431582.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            complement(17578..17850)
                     /gene="yeaC"
                     /locus_tag="N7C74_001701"
     CDS             complement(17578..17850)
                     /gene="yeaC"
                     /locus_tag="N7C74_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HGU2431583.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(17892..18305)
                     /gene="msrB"
                     /locus_tag="N7C74_001702"
     CDS             complement(17892..18305)
                     /gene="msrB"
                     /locus_tag="N7C74_001702"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HGU2431584.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            18647..19642
                     /gene="gapA"
                     /locus_tag="N7C74_001703"
     CDS             18647..19642
                     /gene="gapA"
                     /locus_tag="N7C74_001703"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGU2431585.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            19726..20610
                     /gene="yeaD"
                     /locus_tag="N7C74_001704"
     CDS             19726..20610
                     /gene="yeaD"
                     /locus_tag="N7C74_001704"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HGU2431586.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDANGVALTFELTQSEETKKFWLHDFTLLAHFRVDKNCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASEMQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(20661..21515)
                     /gene="yeaE"
                     /locus_tag="N7C74_001705"
     CDS             complement(20661..21515)
                     /gene="yeaE"
                     /locus_tag="N7C74_001705"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HGU2431587.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAVMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(21605..22351)
                     /gene="mipA"
                     /locus_tag="N7C74_001706"
     CDS             complement(21605..22351)
                     /gene="mipA"
                     /locus_tag="N7C74_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HGU2431588.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNSNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPIVDKSWTGLISTGITYKF"
     gene            22787..24721
                     /gene="yeaG"
                     /locus_tag="N7C74_001707"
     CDS             22787..24721
                     /gene="yeaG"
                     /locus_tag="N7C74_001707"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HGU2431589.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            24834..26117
                     /gene="yeaH"
                     /locus_tag="N7C74_001708"
     CDS             24834..26117
                     /gene="yeaH"
                     /locus_tag="N7C74_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HGU2431590.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT         6747 a         6761 c         6143 g         6588 t
ORIGIN      
        1 cgataatttt atccatgcaa aaaaaatatc caacgaattt tcttgatccg ctggatattt
       61 cagaatatga ctcgaatagc acgaaagatt cgctcgctta cgctatcgcc ccgcttccgg
      121 cttcatctgc tggcggactt tttttcgcac tacgtttacg cggtgcagcc tttttcttat
      181 cagcactgcc accactgccc ggagccacaa tgccgcgaaa ctgccgcacc ggagtacgtt
      241 tggcttgatc aataagctga tatagcgtcc ccaccagcgg ctgcataaag tcctgatagc
      301 gacactgctt ttcgctgatt tgcgtcagca ccgattccca gtgcgcggtc atgtccggtc
      361 gcgtagccat ttctggcagc gaatggaata acgcttttcc ggcgtcggtg gagtggatat
      421 agcgcccttt tttgatgagg aaaccacgct tgaacaacag ttcaataatc ccggcacgcg
      481 ttgcttctgt ccccagacca tcggtcgcac gaaggatctt tttcagatct ttgtcctgca
      541 caaagcgcgc gatcccggtc atcgccgaaa gcagtgttgc atcggtaaaa tggcgcggcg
      601 gctgggtttg ccgctctacc acttcacctt tttcacacag caactcatcg cctttcgcca
      661 ccacaggcaa tggcgtaccg tcgttttctt catcgcgctc tttgctgcct aacagcgtgc
      721 gccagcctgc ttcagcaaga aaacgcgctt tagcgacaaa tttgcctttg gcaatgtcca
      781 gttcgataac acacttgcgg aacaccgcat ccgggcagaa ctgcatcaga tactgacggg
      841 aaatcaggtt atagaccttc gcttcgttct ccgtcaggtt gatcgcagaa ctccgtgcgg
      901 tcggaatgat ggcgtggtgc gcatcgacct ttttgtcatc ccaacagcgg ttgcgtatat
      961 ctggatctac cactggctgc ggcaacagat ctggtgcatg aacgctgatg gcattcatca
     1021 ccgcgtggcg tccggcaaaa tgttcttctg gcaaatagcg acaatcggaa cgcggataag
     1081 tgattagctt gtgcgtttcg tacagcttct ggcagatatc aagcacgttc tgcgcgctca
     1141 gaccaaagcg ttttgccgct tcaatctgca acgccgaaag cgaaaacggc agcggcgcgg
     1201 attctgattc ccgtttatcg ttatagctgg tgacaatagc cggttgacca ctaatgcggt
     1261 taaccacatg ctccgccagt ggacgatgta acaagcgccc ttcttcatcc tggtacggtt
     1321 cacacgcttc gctcggttgc cagatagcgg taaaccgctc atcggcaggt gtcacgatat
     1381 gtgctttgac ttcaaagaag tctttcgcca cgaagttttc aatctcttca tcgcggcgca
     1441 ccaccagccc aagcacgggc gtctgcacgc gtcccacgga aagcacgccc tgataaccgg
     1501 cattgcgacc gagaatggta tacgcacggg tcatattgat gccgtacagc cagtcggcac
     1561 gcgctcgcgc cagcgcagaa acgcacagcg gtacaaactc actgttggaa cgaagacggt
     1621 cgatcgcccg ctcaaccgcc tgcgggttca ggtcgtttat caagcaacgc tgcacctgct
     1681 ggcgcttttc cggtgccagt tgtagatagt ccagcacttc atccaccagc agttgccctt
     1741 cgcgatccgg gtccccggcg tgaacgattt cgctggcttc atgcaggtac cgtttgatga
     1801 cgttaagttg tttggtcacg gagggtcggg gctgtaattg ccacttttcc gggacaatcg
     1861 gcaaatccgc aagattccag cgcgcatagc ggctgtcgta ggcgtctggc tgcgcctgct
     1921 caagcaggtg accgatacac caggtcacca cctgaccatt accgcactcg ataaagccat
     1981 cgcctttccg gtgcggtttg ggcaggacat cagcaatggc gcgcgccaga ctcggttttt
     2041 cggcaataaa caaccgcatt gaattaacgg atctcaacca tggcacgacc gccgcgtgct
     2101 ggcaccagtt cgccaatcgc cgtcagttca atgccaaact cggcggctgt agctttgacc
     2161 tcattttcgg cttccggcat gaccgccagc agcaaaccgc cagaagtttg cggatcgcac
     2221 agcaaatcgc gcacttcacg cggcatttca cccatcagat gaccgtagct ggcaaagtta
     2281 cgttcggtgc cgccaggcac tgcacccaac ttaatgtact cttcaacccc cggcaatttc
     2341 gggatcgcgt catagtcaac gcgcgcctgc acacctgcgc cctgacacat ttcgctcaaa
     2401 tggcccagca ggccaaagcc cgttacgtcg gtcatcgctt ttacgccttc aatgttggca
     2461 aaggacgcgc ctgcgatgtt catccggcac atcacttccg tcgccagtcc ctgatgttct
     2521 ggtttcagta gtgatttttt ctcagccgtg gtaagaacgc cgatccccag cggtttcgtc
     2581 aggaacagtt tacatccggc ttgtgcggta ctgtttttct tcacccgctc ggtcggtacg
     2641 atccccgtta ccgccagacc aaaaatcggc tccggcgcat cgatggagtg accgccagcc
     2701 agcgcaatac ccgcctgacg gcatgcatag cgtccacctt cggttacttc gcgggcaatt
     2761 tccggggaaa gtttgttaat cggccagccg aggatcgcaa ttgccataat cggtttgccc
     2821 cccatcgcga agatatcgct gatggcgtta gtcgccgcaa tgcggccaaa atcgaaagga
     2881 ttatcaacga tcggcataaa gaagtcggtg gtactgataa cgctggtgcc attgcccaga
     2941 tcgtacaccg ccgcatcgtc gcgggtttca ttacccacaa gcaaattcgg atcaacaaac
     3001 ttcgcctgct cactgtgcag gatggtttcc aacacttttg gggaaatttt acagccgcaa
     3061 ccagctccgt ggctgtattg ggtcaaacga atcgagttct cgctcatgga catctcctgt
     3121 caatgcaatc cgggtatggt aaccctcatt ccgtgaagtg ataagtgaga gtgtctgaat
     3181 tcctgcgcct ttgctcacaa tccagacagt ttcgcgacaa ttatcagaaa taagtgacaa
     3241 acggcgtcgg gtccgggacg tttatcgacg tagatgcttt cagctgcggc gtgccgaggt
     3301 agagaaaacc gacaatttta tcctgctcac ggcaaccgaa tgcttcacgc actaccggac
     3361 tttcagttaa tgcgccactg cgccagatgc cgccaaaccc ctgggcaacg gctgccattt
     3421 gcatcgccat gaccgcgcat ccggcagaca tttcctgttc ccatcgcggg actttatgat
     3481 tttcttcgca tttcgccacc accgtgatga tgagcggtgc gcggaacggc gcattacggg
     3541 ctttgtcgat agctttgtca tcactaccgg cagcaatcgc cccctgttcc agtacggcgc
     3601 tgaaacgctc gcgcccttcc ccttcaatca caaaaaaatg ccacggttgc atggacttat
     3661 ggtccggcgc gcgcataccc gcacgcagga tgttttgcag ttgttcaccc gttggtgcgg
     3721 gttcagccag gcgggaggcg ctacggcgat tgatcaatag ttcgagtgca tccatttgat
     3781 taactcctgt cgtgatattt attcacaaaa ttaacacgag agtggatttt gttacagcac
     3841 agtccgcaat tcctgctgac aagtgccggt tgggtcatta cgataaccac atctattgcg
     3901 cctgtgacag gtgtgacctt aagttgggag aatacatgcg aaccctttgg cgatttattg
     3961 ccggattttt taaatggacg tggcgtctgc tgaatttcgt ccgtgaaatg gtacttaacc
     4021 tgttctttat ttttctcgtg ctggttggtg tggggatctg gatgcaggtc agtggtggtg
     4081 attcgaaaga aacggccagt cgtggtgcac tgctgctgga catttctggt gtgatcgtcg
     4141 ataaacccga cagttctcag cggtttagta aattaagccg ccaactactt ggtgccagtt
     4201 ccgatcgtct gcaggaaaac tcactgtttg atatcgtcaa taccattcgc caggcgaagg
     4261 atgaccgcaa tatcaccggt atcgtgatgg atctgaaaaa cttcgcaggc ggcgaccaac
     4321 cgtctatgca gtacatcggc aaagccctga aagagtttcg tgacagcggg aaaccggttt
     4381 atgccgttgg cgagaactac agccaggggc aatattatct cgccagtttc gccaataaaa
     4441 tttggctgtc accgcaaggc gtggtggatt tgcacggttt tgctaccaac ggtctgtact
     4501 acaaatcgtt gctggataag ctgaaagttt ccacccatgt gttccgcgtg ggtacgtata
     4561 aatctgccgt tgaaccgttt attcgtgatg acatgtcacc ggcagcccgc gaagctgaca
     4621 gccgctggat tggtgagctg tggcaaaact atctgaatac tgttgccgct aaccggcaga
     4681 tccctgctca gcaggtattc cctggcgcgc aagggttgct tgagggttta accaaaaccg
     4741 gtggcgatac cgcgaaatat gcactggaaa acaagctggt cgatgcactg gcatcgagtg
     4801 cggaaatcga aaaagcactg accaaagagt tcggctggag taaggctgat aaaaattatc
     4861 gcgccatcag ttattacgat tacgcattga aaacgccggc agataccggt gacagcatcg
     4921 gtgtcgtctt cgctaatggc gcaattatgg atggcgagga aactcagggg aatgttggcg
     4981 gtgataccac tgcggcacaa atccgcgacg ctcgccttga cccgaaagtg aaagcgattg
     5041 tcctgcgtgt taatagccca ggcggcagcg ttaccgcgtc tgaagtgatt cgcgctgaac
     5101 tggcagcagc ccgggcagcg ggtaagcctg tggttgtatc gatgggcggc atggcggcat
     5161 ctggtggtta ctggatttcc acgccagcta attacattgt ggctaacccc agcaccctga
     5221 ccggttctat tggtatcttc ggcgtgatca ccaccgtaga aaatagtctg gattcgattg
     5281 gtgttcatac tgatggtgtc tcaacttcac cgctggcgga tgtttctatc accagggcac
     5341 tgccgccgga agcgcagcag atgatgcagt taagcattga gaatggctat aaacgcttta
     5401 tcacgctggt tgctgatgcg cgtcattcga cgccggagca gattgataaa atcgcccagg
     5461 gccacgtctg gaccggtcag gacgcaaaag ctaacgggct ggtcgatagt ctcggggatt
     5521 tcgatgatgc ggtcgccaaa gcagcagagc tggcaaaagc gaaacagtgg catctggaat
     5581 actacgttga tgaaccgacc ttcttcgaca aagtgatgga caacatgtct ggttctgtcc
     5641 gggcaatgtt gccagatgcg ttccaggcca tgttacctgc accgctggcc tcggtagcct
     5701 ctaccgttaa aagtgaaagc gacaagctgg cggcgtttaa cgacccacaa aaccgttatg
     5761 cgttttgcct gacctgcgcc aacgtgcgtt aaatcctgca aaaatgtggc cgatatgctg
     5821 tcggccacac tcctgaatcg gactgtcttt cactccctct ctccactaca ttcccttgct
     5881 catttagcat ctgatatgga gtcatcagtg aacaaggccc cctctttaat tgccgctatc
     5941 gtcctcggac tggggattag cgcctgtggt tattttgttg gcgatggtgt taaacattta
     6001 aaaaccaaca accgttatgt caatgtgcgc gggctttctg aaaaagaagt tcgcgcagat
     6061 acggcggaat taacaattgc gattaacttt aagggtaatg ttcccggcga actgttcccg
     6121 aaactggaag aagcgcagaa aaaaattgtt gccgaactca atgctcaggg gattaacgag
     6181 aaagagataa ttctgggcca atggacaagc aagcgaacag attcctttta tctgaaagat
     6241 gatcccacaa tgccacgcta taacgcagac gggtcagtga cgataaaaac ccataatgtt
     6301 gctgctgtcg aaaaagtcgt cgcgaaatta aacgaactgc aagtcgctac ggatggcgct
     6361 atcgctgaga gcaaagtcgc ctatcgcttt aatggtattg gcgcactgcg cgctgagatg
     6421 attgctgccg ccaccaaaga tgcacgcaac gccgcactac aatttgcaac ggactcaggt
     6481 agccaggtgg ggtcaattag cgatgcctca caaggcgtat tccagatctt tgccagtggc
     6541 agtgatgaag atgatcctac ggcgattaat aaaacggtac gcgtagtcac gaccgtaacc
     6601 tatgcgttgc aggattaacc acttttctcc ggcgggacac taatccctcc gtcctgccgg
     6661 acttttctca tatccacaga gtatccaggt gcgctcgctg cccctcacgt atatactttt
     6721 gctctttcga tatcattcat atcaatatca tgcaaaagaa atcaatttac gttgcctaca
     6781 cgggcgggac catcgggatg cagcgttccg agcagggtta tataccggtg tcaggtcatc
     6841 tacaacgcca actggcgctg atgccggaat tccatcgccc ggagatgcca gatttcacca
     6901 ttcatgaata tacgccgctg atggattctt cagatatgac gccggaagac tggcagcata
     6961 ttgctgaaga tattaaagcg cactatgacg actatgatgg ttttgtcatt ctgcacggca
     7021 ccgacacgat ggcgtatacc gcctctgcgc tgtcgttcat gctcgaaaat ctcggtaaac
     7081 cggtcattgt gacagggtca caaatcccgc tggctgagtt acgctctgac ggacaaatta
     7141 atctgctgaa tgcgttgtac gttgcggcga attatccgat caacgaagta acactctttt
     7201 ttaataaccg actgtatcgc ggcaaccgca ctaccaaagc ccatgccgat ggttttgatg
     7261 cgtttgcctc tccaaacctt cctccgttac tggaagcagg tatccatatt cgtcgtttga
     7321 atacgccacc cgccccgcac ggtgaagggg aattgatcgt acatccaatc accccacaac
     7381 caattggcgt agtgacgatt tatccaggga tttctgctga cgtcgtgcgc aattttctgc
     7441 gccaaccggt gaaagcattg attctgcgct cctatggcgt gggtaatgcg ccacaaaaca
     7501 aagccttcct gcaggaatta caagaagcca gcgatcgcgg tattgtggtg gtcaacctga
     7561 cacaatgtat gtccggtaaa gtgaatatgg gtggttatgc caccggtaac gccctcgccc
     7621 atgctggcgt tattggcggt gcagatatga ctgtagaagc cacgctaacc aaactgcatt
     7681 acctgcttag ccaggaactg gatactgaaa ccattcgcaa ggccatgagc caaaacctgc
     7741 gcggcgaact gacgccggat gattaaggag actgcaatgc cccctcgcgc cctgttactg
     7801 gtcgatttac aaaatgattt ctgtgctggt ggcgcgctcg ccgtgccgga aggtgacagt
     7861 acggtggatg tcgctaaccg cctgattgac tggtgccagt cgcgcggtga agcggttatc
     7921 gccagtcagg actggcaccc ggcgaatcac ggcagttttg ccagccagca cggtgtagag
     7981 ccttatacgc caggccaact cgacggtttg ccacaaacct tctggccaga tcactgtgtg
     8041 cagaacagtg aaggcgcaca attacatccg ttactgaacc aaaaagcgat cgcagctgtg
     8101 ttccataaag gcgaaaatcc tttagttgac agttacagtg ccctttttga taacggccgt
     8161 cggcagaaaa cctctctcga tgactggtta cgcgatcatg aaatcgatga attgatcgtt
     8221 atgggcctgg ctactgacta ttgcgtgaag tttaccgtgc tggacgcgtt acagttaggt
     8281 tataaggtaa acgtgattac cgatggttgt cgtggcgtga atatccagcc ccaggacagt
     8341 gcgcacgcgt ttatggagat gtcagcagct ggggcaacgc tatatacgct ggcagactgg
     8401 gaagagacgc aggggtaatt ttacgctggc ctacaattct gtactggcat tgtaggccaa
     8461 ataaaacacg tcagtggcac atctggcaat tgatgccatc aacgaaagat ttaatttacc
     8521 tcagaaatct cttccaacga caccttccgc gtttcgatgc caaaaatgga gagaaccagc
     8581 gcacagagca ataacatgac accaagtacc ataaacaccg tgatcgaccc ataatgtgtt
     8641 aataatgccg caacgccata gggcgtgaag actgcgacga tccgtccgac ggcattaacg
     8701 aaacccgaac cgcgcaggcg taaatgcgtt ggccaaagct ccgggatata aaccgccgac
     8761 gcgaagcaaa catacatgta taaaaagaag atcatcacca ggccatagat taaaatcgcc
     8821 cactctgtag tctgaattga atagatatag cctaacactg caataataat aagtaaggtg
     8881 gagccaaata accgacgagg aaaatgatca ataattaatg ccgcaataaa tattcctacc
     8941 ggagcgccaa tcataataac agcggtcatt aatattgatt tatcgacatc aatgccggag
     9001 ttaacaaata tggtcggtat ccatacggtg atggtataaa gcgaaatgtt cattgcaatt
     9061 aaaacagtaa tcgcgactaa tgtacgtcgt aacatttcac ctttaaacag gagccagaaa
     9121 gtacccttga ttactttaac cttgctgttg ctctgatacg aagtaagcgg gggtaaacga
     9181 atactcttct ctctttcaat ttgctgctct acttcacgaa gttggcattc tgcacctgcg
     9241 atttgccctt tccctgccag ccatcgtggc gactcaataa agtatttacc tgagagaaac
     9301 caggctaaca gtatgccaat accacccaac agaaacatta tccgccaact aaaaaaagcg
     9361 ataaccacca cacctatcgc cgcagacagc atgggcgacc agttaccaac aaatgagagc
     9421 cgcgcggacc attttccacg caccgtcgcg gggataaact ccgtaaatga ggcatagcca
     9481 accataatca gcgcccccat tcctgttccc atcaggaagc gaaagaagat aagccagtac
     9541 atatcaggga caaaagcggc ccctgttgca gcaataccga cgatgagaag atttatgcga
     9601 aacgccctgc gccgcccaaa gtaatcaccg atgaacccac cggtaagaga gccgatgaaa
     9661 taaccaaaca ttaatgccga ggtaaaggcg gcattgagga aattatttga ccatccattg
     9721 cttaccagct ttgctaagac gacattaccg gagtaactca aaaaccctgt taataacaga
     9781 ctaaagctta taataccaaa tatacgataa tgaaaccggg ccaaaggcaa gcggtccagt
     9841 cttgcgccaa tttgatcata ttgttccatt gggtgcctcg gatttgctaa aaaaggtagc
     9901 aattgcggca aattcacttg ccgcatcttt atcatcattt tatttccagg cgcaggaaca
     9961 aataatattg acaacgcgtg cttaaattac cacaccagct ggatattatt gtctttgcaa
    10021 aatgctatcc actctgcacc cggtgattta tcagttatta tgtaattaat atgactaaag
    10081 tcggctaact ggacaaaagc tttgcgatca aacttagagt gatcaaccaa taacgcaact
    10141 tctgtcgcct gacggatcat tgtctttttg atttcagctt ccgcttcatt agagtccagc
    10201 gcgccgctgt taatatcaag acctttacaa ctcattacca tgatatcgac atgatagcgg
    10261 ctgatgatct ctttagtaat tcttccttgc agtgaaagtg tatttttgtt tagttcccca
    10321 cccgttgaaa cgactttaat ttctgactga gccagaacat gaatcgcttc cgccgagttg
    10381 gttagcaacg tcaggtcact acggtcctgt aacaatttga gcaattccat tacggtacta
    10441 ctggaatcgg ctgccatggt ggttttattg tcgataaagg gtagtgcctt gcgtgcaata
    10501 agctgcttct cttcataaaa cgatgaagcg cgcttataaa aatggatatt ctccgtcaac
    10561 atcgctgtat ttaaaacagc accaccatag gttctggtca aaaagccttc atcttccagc
    10621 ttctcaagat cgcggcgaat ggtttcttcg gttacctgaa aaatcccact caaatttgag
    10681 actgtcacct ttttatcgtt ggcaaccatt tgcttaattg cctgaatcct gtcttttgcc
    10741 gccacgatta cacccctgta tctttttaca tcacattagc gcgattatcg cataaccgat
    10801 gtttactttc aaaataacct gtttgaatca cagattttca tcacagtttt cacagaaaca
    10861 gaggtgaatc gtgttgagta tttaacgccc caagacctct gccatttccc tcatcaatgt
    10921 tgcgtctgca tccgataagt tgatattcag tgccgcgaca ttttcgcgta cctgttccgg
    10981 tgcagtagcc ccactaagaa tggagattaa atcactctgt tttaatatcc acgccagtgc
    11041 cagagtggga attgtgcact gataacgagc acaaagtggc tgccactgtt caagcatatc
    11101 aatcactttc agcatgtttt cacgctggaa ccagacttta tttgcccgag cgccgcccgg
    11161 aacgtaatca cgagtgatgg tgccggtcaa caatccctgc tctagcgggg aataaacctg
    11221 aaccacaatg ccattatcac gacatagcgg cagcagttcg ttttccattg cccggtcgag
    11281 gatactgtat ttcgcctgaa taatatccag ttcaccatat tgcagatact cgcggatatg
    11341 gtcagcatcg acgttagcag cgcctatagc gcgaattttc ccttcagcct ttaactcatt
    11401 aagcactgcg acagtttcag cgatcggcgt ataaaatggc ggcaccgact gccagtgcgt
    11461 catgtagata tcgatgtaat caaaacccag acgttgcaag ctggcttcta cctcttcgcg
    11521 gatagattcc ggggaaaggt ttttatacaa ctgccgatcg ccaactttgt tgaataaact
    11581 tccttttcgt tcccagacaa tgccgcattt ggtttctact acaacctgtt cacggggcag
    11641 tttttttaac gcctgaccga cgataacttc actattgcca aagttatatc ctggtgcagt
    11701 atcaatcagg ttaatgccgc aacgatgggc ttcaagaatc gtatcaatac atatttgccg
    11761 atcgagatcg ccattccatg caggaccgcc gccaatggcc catgtcccca accccattcg
    11821 cgaaagcgta atatccgttg tgcctaaagg tatctttttc atttgccttc attatccttc
    11881 gtattcttcc agcaattgtt cgaccagctt tctgtttttt acgccggtgg tggcaccgac
    11941 gcttagaacc gagatagccg ccgttgcatt ggcaaagcgt gcgcattcac gcagattttt
    12001 tccttctaac agtgccgcaa taaaacctga agcaaagtta tcgcccgcgc caatggtgtc
    12061 gatggcggtt attcctgcga ccgccggcac cttcatcgtc atgtcaccac gcttgataaa
    12121 gcagccgtct ttacccgttt taatcaccac cgtttttacg ccgcacgcaa gaaagctgtc
    12181 agcaatttca tccagtgtct ctttcccggt gagtaatttt gcctcggcat aattgacata
    12241 actcaatgct tcgcaaatat catccagcgt ttcattcaac cgcggtttaa tcatatcggc
    12301 acagatattc atctgccggg ctttagcttg cgtaaaaatt tctgttagcg ctttaccatc
    12361 caatagtgga ctgttgaaaa tactggccag tgataataat ttcgcctggg aaaaccgcgc
    12421 aaaatcaaca tcgtcaatat tcaatttcca cagactgccg ttacggtttg tgacaaacgt
    12481 ccgctcgcca tcctccgtca ccagtccaac attaatagag gtatctatgt tgacgtcctg
    12541 cttcaggctt tgaatatcaa tattctcttt gcggcaatgg tcgagaataa actgccctgc
    12601 ggcatcttta ccaatacggc tcattaatgc tgtacgatgg cccagacgag aaataattgt
    12661 tgcttcattt attgcatcac ccccggtggt cattgcgatt ctttcaagag gataagaatc
    12721 cacatcaaag atatttttac tgaccggttg caatggaata tcaacaatag cggcacctat
    12781 acaaataacg tcgagattat ccatatcatt attccgcttt gccatcagaa ccgaacagtt
    12841 taattttttc caatgcccgt tcttttacag ctttacgtac ttcacgttct aaatgcagga
    12901 aaggctgatc ctggttttct ttcactgcga ccattgcggc ctgacagagc tccgtatgga
    12961 tgttgatttt cgcgataccc aacgaaattg cagttttaat gtcggcatca ctgatccccg
    13021 atgcgccatg caaaaccagc ggaacagaca ctgcatcgcg tacgcgtttg acgacctcaa
    13081 agttcaattg tggctctgac gtataaaccc catgctggtt gccgatggcg acagccaatg
    13141 agtcacagcc cgtacgttca acaaattcag ccgcctggtc aggatcggta tagtgatagc
    13201 ccgccagcgc ttcctcataa acggtttcat taccgacatg ccctaattcc gcctctaccg
    13261 gaatacccag cggatggaag aaatcaacag cctctttggt taaacgaata ttttcttcga
    13321 aatcaaatgc ggaagcatca cgcattaatg aattcatacc atgagtccag gcgttatgaa
    13381 taatctccat actccgacca tgatcccaat gagttattac cggcaccgtt gctttttgtg
    13441 ccatagatac catcatgtga gagaaatctt caaatgaggt gttgccgaca aaacctgtgc
    13501 caaaagaaat aataaccggt gatttcgctt cttcggctgc gtcgataacg cccatcaaca
    13561 tttctgcatt ccatacatta aaatgggcaa tagcataacg tttatttgtg gcatcgtttt
    13621 cccaatatct gatatctgcg agcatgttct gttctcccgt attaattaat cgctaatctt
    13681 aataacgcct ttaataatgt cgcgtttgtt gttaactgac tcttcaaatg cctgttgtac
    13741 atcccgataa tcgtaaatat gcgtcaccat cgatttcaca tcgaatcgcc ctgaagaaat
    13801 tgcttcaatc gtgaccggat aacgattggc atagcggaat accgtctgga tagtgacttc
    13861 gcgattgatt ttgaggaaat tgattgccga agcgccgggt acagtaccaa caatcataat
    13921 tttaccgcca cgcattacca gataaggtgc ctgtttaacg gtgaccgcag aacccgctgt
    13981 ttcgaaaaca atatctgcgc ccatgtcttc ggtaaattgc tgacagcgtg caatagtgtc
    14041 ttcttttgcg ccgttaataa ccactgtcgc accaagctgt tccgccattg ccagacgttt
    14101 ttccagcaca tcaacgacgg caatttccgt tgctcccagg catttgcacg cttgcaacgt
    14161 catcaaacca atacaacctg ctcccagaat aattatcttc ttacccggtt taacatctgc
    14221 cagcatcgcg gcatgcatcc cgactgcggc aggctccacc agcgtccctt ccatcgtgtc
    14281 catattgtcg ggcagtttgt aagtaaagct ctccggatga cacagatagt gcgttaatgc
    14341 gccgcggtag ttgggttgtg tcgccataaa atcaacgtcc gggcagatgt tatatttgcc
    14401 ttccagacag taacgacagt gaccgcaagg aacgccaggt tcgatattta cccgatcccc
    14461 cggtttaaat ttgcgcacac ggcttcccac agccacaacc gtcccggcgc attcatgacc
    14521 caggccaatt tcttgatttg ggtctttagg cggaataaac ggacctgatt caaaaccatg
    14581 tacatctgaa ccacaaatac cgacatattc tactttaatc aaaacttcat cttctttagg
    14641 cactggtatt tctgctgaaa taattttcat tgtgcccggc acctgcaata ttgcttttga
    14701 atttttcatt ttgaatccac ccgtattatt tattggctac tgcatcaata cttaaacttt
    14761 caacggagac acctttggtt tcgattccaa tggtggcgat agcaattgcg acaataattg
    14821 aaaccgcccc cagaagaata aagactcccg taacgccata actactgagc agcactgcaa
    14881 cggcataagg tgcggcaata ccactgattc gccctaccgc attcgccaga ccggagccac
    14941 ggagtttggc ctctgtcggc cagatctcag ggacatacac tgccgaggca tagcaaacgt
    15001 acatatagac gaaagtaatc aggaagaaac caatcaacgt tatgagcaac atgctggttt
    15061 gcagtgaata gatatatccg agcaccgcaa tcaggattaa tagccccaca cccattgttt
    15121 tacgcggaat tttatccatc accagcatgg caataaaaat gccaaatggc gtgccaaaca
    15181 tactcatggt atttaaaaca atcgagtctt ttaaattaat cccctgggtc atgaatatcg
    15241 ttggcaacca gttaattagt gtgtactgca caacgttcat ggcaatcagc acacaagagc
    15301 ctaatatcac gcgtttcagt aatactcctg tcagtaaggc tgaatatggc accgcttgtg
    15361 gtgctttacc gtcatcagca ataaccacgg gcggcaaagg tttacctgta tggcgtatga
    15421 cgccttcttc tatactccgc atgactttct cggcttcctg ataccgtccg cgcgattcca
    15481 gccagcgcgg ggattcagga aagtagcgcc aggccagcgc cgtagcgata agcgacaata
    15541 ttgcagggat aagcagttgt acccgccagt tccactctgc actaatcagc ggcgtgagtc
    15601 ccatcgctat caatgaacag agcggatatg accagttgcc aataaaggaa acccgacttg
    15661 accacgttcc acgatttcta ccgggcatat attcggtgaa accagcaaac agcgtaacca
    15721 gtaaagctcc cagcccaact cccatcacaa aacggcaggc aataaggaaa tccatattcg
    15781 gtgagaaagc accgacaacc atcgaggcaa tatgaatggc ctcatagagg atgaacgcat
    15841 ttctgcgacc ggttttgtcg ccaatgatgc cgcccaccag cgcaccgaga aacattccgg
    15901 cggtcgtgat tgctgagaat gtggctgtgg tggaattatc tgtccagccc aacgctttca
    15961 gctgcgcgag gatcaagcca ccaacggcat tactccagca gacaagcaag ccaaacgcga
    16021 cgatggcaaa cattgatgaa tgccagcggc aatccggtaa gcgatccagc cgtgcaccac
    16081 aatgcggttt tgttatctga tccatgcgat aacgttcctt caggagagtg gttattcatc
    16141 aaagtcgtaa gtcatgatca ctttgattgc ggttttatcg accatcgcat caaacccttc
    16201 gcgccattgc gacaggccga tacgatgcgt gatcatcggt ttgactttga tagcgccgct
    16261 ggctaatagc ctgatagcgt tacgccatga ggtggagtca taggccatat gcccaatgat
    16321 gcttttgttc caggcggtaa tgtcattaat cgagaaatca agaggtttga agcccattcc
    16381 aacgcgtacc acttccccgt tcggacggag catttcgatg gcttgtttca gtgcgatatt
    16441 ggcaccggag cattcaatca ccagtcccag attgtctttg ccacaaattt gctggcagcg
    16501 cgccaccaca tcttcggtag aaccatttac tactgccgtc gcacccagtt cttttgcaac
    16561 cgggaagcgg accgccacat cttcttgcag accaacgacg acgatattta ccgcccccat
    16621 aattcgcgcc atttgtacgg agaacagccc gagtgggcca gtgccgatga cgaccacatc
    16681 ctgaccaggg aggaatttcg attgctgcgc gatggatttg taggcattac agatagggtc
    16741 aagtacggct gcgtcctcat aatcaacacc atcagggatt tcccacaacg catgacgatg
    16801 aattttgaga atttcaccag gaaccagaca atatttggaa aaaccaccgc cccaggtatt
    16861 gttatccaga ccaaggttta ctttttctgt acaacacaga aaatcacctt gttcacaggc
    16921 cggacaaacg ccgcagacgt gaccgctgtt atccgacacg acgcgttgcc ccactttcca
    16981 gtctttgact ttttcaccaa cctgcgcaat acaacctgcg aactcatggc cgcggataga
    17041 gttaaactca tcagaaccgc tatcgacatt gtagtgcttc atgtctgcgc cgcagattgc
    17101 cgcggcttta atttcaatca ctacatcttc cgggccacaa atgggttgtg gcacatcgat
    17161 catcttgtag ccgccaaagg ctttgccaaa ccgagccagt gctttcattg cgtcatcctc
    17221 tgtgttttgt tttgcttaga tggtcatcat acttacccac atgatttttg aggtcaacat
    17281 taaacatgta aaacacagat aataatctgc gttttacaac tcagatcaca attacgcaga
    17341 aaatggctgg ttctgttaca ggttgatgta aggcggaacg caaaaggagc aaatttgaga
    17401 gggattaccg gccaaatagg tctgagactt aagttcagga gaagtcattc aggcgtgaac
    17461 gcgacggcaa gagatcggac tgggacttag ccaggtcatg gcgagataat tgagatgagt
    17521 cgtggggtca ttgttggcta tccccttcat agccgcggca aaaaggggat gaaccactta
    17581 cttaaacatg gcaataggct ttgctgagat accaaaatct tcttttaact gctgtttgct
    17641 cttcatgacc atctgaccat ttgtgtcaat tgtcatatgc tgtgcttcgg tattattgcg
    17701 ggcttgccac agcatcacca gttgcaggca gttctctttt tgttcctcgg tcaacgcaac
    17761 gccatcaggc catttcccca gttcaacggc ggtcgacaaa cgctggtata cctcaggcgt
    17821 catgctgttg ataatgtcat caagattcat aatttttccg ctcctgtgga ataagttgct
    17881 gaatcgtttt ttcaaccgtt gatttcttcg ccgttttcac catcagtgaa gcgtaaagaa
    17941 gcagagttaa cacaataacg ttcgcccgtt ggctgcggcc cgtcagggaa gacatgcccc
    18001 agatgggcat cacagttacc gcaacgaatt tctatgcgct gcattccatg tgacaagtct
    18061 ttgatataac gaatggattc ttcactcacc ggttcgtaga aactgggcca gccacagccg
    18121 gaatcatact tggtttggga atgaaacagc ggggcatcgc agatcaaaca gtgatatacg
    18181 ccgtcacgct tgttatgcag taaacgaccc gtaaatggcg gctctgtccc atgattctgc
    18241 gtcacgtaaa actgcatctc ggacaaattt tttttcagtt cttctgccga aggtttatta
    18301 gccatttgct cacatctcac tttaatcgtg ctcacattac gtgactgatt ctaacaaaac
    18361 attaacacca actggcaaaa ttttgtccta aacttgatct cgacgaaatg gctgcaccta
    18421 aatcgtgatg aaaatcacat ttttatcgta attgcccttt aaaattcggg gcgccgaccc
    18481 catgtggtct caagcccaaa ggaagagtga ggcgagtcag tcgcgtaatg cttaggcaca
    18541 ggattgattt gtcgcaatga ttgacacgat tccgcttgac gctgcgtaag gtttttgtaa
    18601 ttttacaggc aaccttttat tcactaacaa atagctggtg gaatatatga ctatcaaagt
    18661 aggtatcaac ggttttggcc gtatcggtcg cattgttttc cgtgctgctc agaaacgttc
    18721 tgacatcgag atcgttgcaa tcaacgacct gttagacgct gattacatgg catacatgct
    18781 gaaatatgac tccactcacg gccgtttcga cggtaccgtt gaagtgaaag acggtcatct
    18841 gatcgttaac ggtaaaaaaa tccgtgttac cgctgaacgt gatccggcta acctgaaatg
    18901 ggacgaagtt ggtgttgacg ttgtcgctga agcaactggt ctgttcctga ctgacgaaac
    18961 tgctcgtaaa cacatcaccg ctggtgcgaa gaaagtggtt atgactggtc cgtctaaaga
    19021 caacactccg atgttcgtta aaggcgctaa cttcgacaaa tatgctggcc aggacatcgt
    19081 ttccaacgct tcctgcacca ccaactgcct ggctccgctg gctaaagtta tcaacgataa
    19141 cttcggcatc atcgaaggtc tgatgaccac cgttcacgct actaccgcta ctcagaaaac
    19201 cgttgatggc ccgtctcaca aagactggcg cggcggccgc ggcgcttccc agaacatcat
    19261 cccgtcctct accggtgctg ctaaagctgt aggtaaagta ctgccagaac tgaatggcaa
    19321 actgactggt atggcgttcc gcgttccgac cccgaacgta tccgtagttg acctgaccgt
    19381 tcgtctggaa aaagctgcaa cttacgagca gatcaaagct gccgttaaag ctgctgctga
    19441 aggcgaaatg aaaggcgttc tgggctacac cgaagatgac gtagtatcta ccgatttcaa
    19501 cggcgaagtt tgcacttccg tgttcgatgc taaagctggt atcgctctga acgacaactt
    19561 cgtgaaactg gtatcctggt acgacaacga aaccggttac tccaacaaag ttctggacct
    19621 gatcgctcac atctctaaat aagttgagat gacactgtga tctaaaaaga gcgacttcgg
    19681 tcgctctttt ttttacctga taaaatgaag ttaaaggact gcgtcatgat taagaaaatt
    19741 tttgcccttc cggtcatcga acaaatctcc cctgtcctct cccgtcgtaa actggatgaa
    19801 ctggacctca ttgtagtcga tcatccccag gtaaaagcct cttttgcatt acagggcgca
    19861 caccttctct cgtggaaacc tgcaggtgaa gaagaagttc tgtggttgag caacaacaca
    19921 ccgttcaaaa atggcgttgc tattcgcggt ggcgtaccgg tttgctggcc gtggtttggt
    19981 ccggcggcac aacaaggcct gcctgcgcac ggttttgccc gcaacctgcc gtggacgctg
    20041 aaatcacatc gtgaagatgc taatggcgta gcgctgactt ttgaattgac acaaagcgaa
    20101 gagacgaaaa aattctggct gcacgacttt actctgttag cgcatttccg cgtggataaa
    20161 aactgtgaaa tcgatcttga agcacacggc gaatttgaaa ccacctctgc cctgcatacc
    20221 tactttaacg tgggtgatat cgctaaggta agcgtcagtg ggctgggcga tcgcttcatt
    20281 gataaagtaa atgacgcgaa agaagatgta ctgaccgacg gtattcagac cttccctgac
    20341 cgtaccgatc gcgtgtatct gaatccgcaa gattgcagcg tgattaatga tgaagcgctg
    20401 aatcgtatta tcgccgtagg ccaccagcat catctgaacg ttgtaggctg gaacccggga
    20461 ccggcgcttt cagttagcat gggcgacatg ccggacgatg gctacaagac ctttgtttgt
    20521 gtagaaacag cttacgcctc agaaatgcaa aaagtgacca aagaaaaacc tgcacatctg
    20581 gcgcaatcca ttcgcgttgc caaacgttaa tttgcgtcaa tgttgtgtgc cgggtgcgat
    20641 gcatccggca cgtatcaaca tcacaccata tccagcgcag tttttccttt tggtgccgga
    20701 tacgctttgt ccagcatggc taattccgct gacgaaagtt cgacctcaag cacagccgca
    20761 ttctgttgga catgggcaat cgtggccgct tttggaatcg ccatcacacc ctgatggctg
    20821 attacccacg ccaacaatac ttgcgccgcg ctgatattgt gagcatgtgc aatttcgttg
    20881 actaccgcgt tttttaacag tccattgcgc aaccgcccgg cctgggctaa cggactgtaa
    20941 gccatcaccg gcatctgctg ttgctggcac caggggagta gatcgtactc aatccctcgt
    21001 gaaccgagat ggtaaagcac ctgattagtg gcacactgat ttccccccgg cagctgccag
    21061 agttcctgca tatcagcata atcaaggttc gaaacgcccc agcggcggat ttttccctgg
    21121 gcgatcaatt tttccatcac tgcaacagtc tcttcaaaag cgaaactgcc agaccagtgt
    21181 aataagtaga gatcgagata atcagtattg agacggcgta aactggcttc gcatgcattt
    21241 atcgcttttt gcccgccagc attccacgga tagactttag agacgagaaa gaccttctct
    21301 cgcagaccgg ttaatgcttc cccaaccacc ttttcggcac cgccatcggc atacatttcg
    21361 gcggtatcaa tgagggttaa accgagttca atgcccgcgc gtagtgcagc aacttctgtt
    21421 ttgcgctgac tggcatcttc gcccatatac catgttccct gccctatggc tggcagtgag
    21481 acatcgccac taaattgaat cattttttgt tgcattgttt cctcccaggt attgcaccac
    21541 cgtaatgcaa aacagggcgt gacgccctgt ttttatgcac aaaatgccct ggaaagatgc
    21601 attatcagaa tttgtaggtg atcccggtag aaatcaggcc agtccaggat ttatccacaa
    21661 tcgggctgtc agtaacttca tcagacagac gggtgtagcg cgcggtaccg taaacactcc
    21721 agtcgccgag gaagttgtag ctggcgctca gctccaggta agggctccag ctgtcgttcg
    21781 agttatagcc acgcagaccg ctgcgagcgg actctttgcg cgatacgcca taatagtatt
    21841 cgttctggtt ttcgctgttc cactgcacac caatacccgg agtcacggtc aggccaccgt
    21901 tggtgtaacg atacaaccag gccatatccc agacgatgcc gttgctgtta tccagggtat
    21961 cgccagccag ggtggtacgc aggtaaccgt actgggtaaa gtgagcataa gacagaccag
    22021 ccatcatggt gctcttacgg tcatccaggt gacgcatttg gtgatcgcca ctgtctttag
    22081 ctttgaagta aagcggcgac cagtaagcgg taattgaaag tttatccgtt gcgtcattcc
    22141 acaggtagta accaccacct aagccacgga accagaagtt atcgccttca tagttgatta
    22201 ccggtactgg gtaaacatcg gtatcgtaat ctttatatgg gtgttcaacg acacctacgc
    22261 ctgcgcccag ggaaaattta ccttcagcgt gcgctacgcc tgcagacgtt gcgataagca
    22321 ctccaagtgc cagaagtttg agtttggtca caattaatca ttccttaaac aaatgtttag
    22381 cgggcgacaa agtttacccg tcaatacatc gaaacccaac ctttttacgt tttcattttt
    22441 taaagtaact gtttaatttt cctgacgcgg atgacaccgc gcttacagcc aaatgaattt
    22501 agcgttactg gcgagcctgg tctttacatt aattatgcaa aatttatgga tgagttgttg
    22561 atatgccatt gaaattaaga aagccgtgta ggcaagtttt ccatttgcca tctacgctta
    22621 attttgaagg tgtatcaccg ggcacgttgt tctcatcgtc gataaaatgg catgagagtt
    22681 gctgtgtttt agcaagagac gtcgttcagt ttacctcttc cgggagcctc tactattcat
    22741 atgaacggct cttaacctgt gctaaaaaac gaaaggacgg cataccatga atatattcga
    22801 tcactatcgc cagcgatatg aagctgccaa ggacgaagag ttcacactgc aggagtttct
    22861 taccacttgt cggcaagatc gcagtgctta tgccaacgcg gctgagcggc tattgatggc
    22921 tatcggtgag cctgtcatgg tcgatacagc ccaggaaccc agactttctc gactcttttc
    22981 taaccgggtc attgcacgtt atccggcgtt tgaagagttt tacggcatgg aagacgcgat
    23041 tgaacagatt gtctcttatc tgaaacacgc ggctcagggg ctggaagaga agaaacaaat
    23101 cctgtatctg ctggggcctg tgggtggggg taaatcatcg cttgctgagc gactgaaatc
    23161 attaatgcag ctcgtaccga tttatgtatt gagcgccaac ggtgagcgta gcccggtcaa
    23221 cgatcatccg ttctgtcttt tcaatccgca ggaagatgcg cagattctgg aaaaagagta
    23281 tggcattcct cgccgttatc tcggcaccat catgtcgccg tgggcggcaa aacgcctgca
    23341 tgaatttggt ggcgatatca ctaagttccg ggtagtgaag gtctggccgt caattctgca
    23401 acaaattgct atcgccaaaa cggaacccgg tgatgagaac aaccaggaca tctccgcgct
    23461 ggttgggaaa gtcgatattc gtaaactcga acattacgcg cagaatgacc cggacgccta
    23521 cggctattcc ggtgcgctgt gccgcgccaa ccaagggatc atggaatttg tcgagatgtt
    23581 taaagcaccg ataaaagtgc tgcatccctt gttaaccgcc actcaggaag gtaactacaa
    23641 cgggacggaa ggtatctccg ccctgccgtt caacgggatt attcttgccc actcgaacga
    23701 gtccgaatgg gtcactttcc gtaataacaa aaacaacgaa gccttcctcg atcgtgttta
    23761 catcgtgaag gtgccgtatt gcttgcgcat ttccgaagag atcaaaatct acgagaaatt
    23821 gcttaatcac agtgaattga ctcacgcccc atgcgcccct ggcactctcg aaacactgtc
    23881 acgtttttcc attctttcgc gcctgaaaga gccagaaaac tccagcattt attcaaagat
    23941 gcgggtttat gatggcgaaa gtctgaaaga caccgatccc aaagccaagt cgtatcagga
    24001 atatcgtgac tacgccggtg tcgatgaagg tatgaacggt ctgtcgacgc gttttgcgtt
    24061 taagatcctc tcccgcgtgt tcaacttcga tcatgtagaa gtggcggcaa acccggtcca
    24121 tctgttctac gtcctggaac agcagatcga gcgcgagcag ttcccacaag agcaggcaga
    24181 acgctatctg gagttcctga aaggttatct gatcccgaaa tatgccgagt ttattggcaa
    24241 agagatccag acggcctacc ttgaatccta ttccgaatat gggcaaaaca ttttcgaccg
    24301 ttatgttacc tacgcggatt tctggattca ggatcaggag tatcgcgatc cggataccgg
    24361 gcagctgttt gaccgcgagt ctcttaacgc cgagctggag aaaatcgaga aaccggcggg
    24421 gatcagtaat ccaaaagatt tccgcaacga gattgttaac ttcgtactgc gcgccagagc
    24481 caataacagc ggacgcaatc cgaactggac cagctatgaa aaactgcgca cggttatcga
    24541 gaagaaaatg ttctccaata ccgaggagct gttgccggtt atctcgttta acgccaaaac
    24601 gtcaaccgac gagcagaaga aacacgacga ctttgtcgac cgtatgatgg aaaaaggcta
    24661 cacccgtaaa caggtgcgtt tactgtgcga atggtatttg cgcgtacgta aatcgtctta
    24721 acaaccctgg cccggtacac atgctaccgg gcctacaacg acagcgaacc gtgggccaga
    24781 gaaatggcaa cacaggcgta gcatacagtt ggcaaatgta gtacgggggg catatgacct
    24841 ggtttattga ccggcgtctg aacggcaaaa acaaaagcat ggtgaatcgc cagcgttttt
    24901 tacgccgtta taaagcgcaa attaaacagt cgatctccga ggccattaat aagcgttcgg
    24961 tgactgacgt cgacagcggc gagtccgtat ccattcccac ggaagatatt agcgaaccaa
    25021 tgtttcatca ggggcgtggc ggtctgcgcc accgcgtgca tccgggcaat gaccatttcg
    25081 ttcagaacga ccgaattgaa cgtccccagg gtggcggcgg aggttccggc agtggtcagg
    25141 gccaggccag ccaggatggt gaaggtcagg atgaatttgt ctttcagatt tcgaaagatg
    25201 agtatcttga tctgctcttt gaagatttgg ccttaccgaa tctgaaacaa aaccaacaac
    25261 gccagctgac cgaatataaa acgcatcggg cgggttatac cgctaacggc gttccggcca
    25321 atatcagcgt tgtgcgttcc ttgcagaact cactggcgcg acgcacagcc atgacggcag
    25381 gcaagcggcg ggaacttcat gcactggaag agaatttggc catcatcagc aacagtgaac
    25441 ctgcgcagct gctggaagag gaacgtctgc gcaaagaaat tgcagaatta cgtgccaaaa
    25501 ttgaacgcgt cccttttatt gacaccttcg atttacgtta caagaactac gagaagcggc
    25561 ccgatccctc cagccaggca gtgatgtttt gcctgatgga cgtttccggt tcaatggatc
    25621 aatccactaa agatatggct aagcgttttt atattctgct gtatctgttc ctcagcagaa
    25681 cgtataagaa cgtggaagtc gtatacatcc gccatcatac ccaggcgaaa gaagtcgatg
    25741 aacatgagtt tttctactcg caggaaactg gcggcaccat tgtttccagc gccctgaaac
    25801 tgatggatga ggtagtgaaa gagcgttata acccggcaca gtggaatatt tacgctgcac
    25861 aagcatcgga cggcgataac tgggccgatg actctccgct ttgccatgaa attctggcga
    25921 aaaaattatt acccgttgtt cgttattaca gctatatcga aattacccgt cgcgcgcatc
    25981 agacactgtg gcgagaatat gagcatctgc aatctacttt cgacaacttt gcgatgcagc
    26041 atatccgcga ccaggatgat atttatccgg tgttccgtga actgtttcat aagcaaaatg
    26101 caacagctaa agactaaaac tatcagccgg gtcattatcg cctggctgat ttttagctca
    26161 ctgtaagtta tctcatttat tacatacatt agcttacaat cgctttaaat atgacagcat
    26221 aacctttaca caatttagt
//
LOCUS       DBFIFM010000047        25887 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.047, whole genome shotgun sequence.
ACCESSION   DBFIFM010000047 DBFIFM010000000
VERSION     DBFIFM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.047"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(83..261)
                     /locus_tag="N7C74_001709"
                     /pseudo
     CDS             complement(83..261)
                     /locus_tag="N7C74_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
     gene            complement(474..1133)
                     /gene="rluA"
                     /locus_tag="N7C74_001710"
     CDS             complement(474..1133)
                     /gene="rluA"
                     /locus_tag="N7C74_001710"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HGU2431591.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFRERVPKKQYVTRVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(1145..4051)
                     /gene="rapA"
                     /locus_tag="N7C74_001711"
     CDS             complement(1145..4051)
                     /gene="rapA"
                     /locus_tag="N7C74_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HGU2431592.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            complement(4215..6566)
                     /gene="polB"
                     /locus_tag="N7C74_001712"
     CDS             complement(4215..6566)
                     /gene="polB"
                     /locus_tag="N7C74_001712"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HGU2431593.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILRGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLVSSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEATKIG
                     RALVQHVNVWWAETLQKQQLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYFRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            complement(6641..7336)
                     /gene="araD"
                     /locus_tag="N7C74_001713"
     CDS             complement(6641..7336)
                     /gene="araD"
                     /locus_tag="N7C74_001713"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HGU2431594.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGAKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            complement(7536..9038)
                     /gene="araA"
                     /locus_tag="N7C74_001714"
     CDS             complement(7536..9038)
                     /gene="araA"
                     /locus_tag="N7C74_001714"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HGU2431595.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVKALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            complement(9049..10749)
                     /gene="araB"
                     /locus_tag="N7C74_001715"
     CDS             complement(9049..10749)
                     /gene="araB"
                     /locus_tag="N7C74_001715"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HGU2431596.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCTTGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQIDASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            11088..11966
                     /gene="araC"
                     /locus_tag="N7C74_001716"
     CDS             11088..11966
                     /gene="araC"
                     /locus_tag="N7C74_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HGU2431597.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            12052..12816
                     /gene="yabI"
                     /locus_tag="N7C74_001717"
     CDS             12052..12816
                     /gene="yabI"
                     /locus_tag="N7C74_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGU2431598.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLVRHPLMPVYIDILRKVVGV"
     gene            complement(12903..13601)
                     /gene="thiQ"
                     /locus_tag="N7C74_001718"
     CDS             complement(12903..13601)
                     /gene="thiQ"
                     /locus_tag="N7C74_001718"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HGU2431599.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGALTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAVQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTNELL
                     SGKASASALLGITG"
     gene            complement(13585..15195)
                     /gene="thiP"
                     /locus_tag="N7C74_001719"
     CDS             complement(13585..15195)
                     /gene="thiP"
                     /locus_tag="N7C74_001719"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HGU2431600.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWS
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPSIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVTALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTMLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFSVIEKIPGRNVKTD"
     gene            complement(15171..16154)
                     /gene="thiB"
                     /locus_tag="N7C74_001720"
     CDS             complement(15171..16154)
                     /gene="thiB"
                     /locus_tag="N7C74_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HGU2431601.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDIFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEKLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     regulatory      complement(16185..16284)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            16526..17095
                     /gene="espY2"
                     /locus_tag="N7C74_001721"
     CDS             16526..17095
                     /gene="espY2"
                     /locus_tag="N7C74_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY2"
                     /protein_id="HGU2431602.1"
                     /translation="MKVRNPEQISIPASNTTKDPGLTNSQIIRMANLVKKTENMNIFE
                     ELWETLRNLFQSDKHSQTAARQILKDAFYFQNSDDYSKYFTGAVDGKARDKLTHWLIK
                     FNELKEYAKDPENMAAKASLSPEGTLCVSFFIGDEAIFTLELQLKKSTRTGGIDLSNA
                     YFNGVVICGIDCLEVDLSNAETNNSRWYD"
     gene            complement(17324..18982)
                     /gene="sgrR"
                     /locus_tag="N7C74_001722"
     CDS             complement(17324..18982)
                     /gene="sgrR"
                     /locus_tag="N7C74_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HGU2431603.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     ITSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQVDAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDPE"
     gene            19071..19202
                     /gene="sgrT"
                     /locus_tag="N7C74_001723"
     CDS             19071..19202
                     /gene="sgrT"
                     /locus_tag="N7C74_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HGU2431604.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            complement(19310..19915)
                     /gene="leuD"
                     /locus_tag="N7C74_001724"
     CDS             complement(19310..19915)
                     /gene="leuD"
                     /locus_tag="N7C74_001724"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HGU2431605.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFSQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMR"
     gene            complement(19926..21326)
                     /gene="leuC"
                     /locus_tag="N7C74_001725"
     CDS             complement(19926..21326)
                     /gene="leuC"
                     /locus_tag="N7C74_001725"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HGU2431606.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGILL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(21329..22420)
                     /gene="leuB"
                     /locus_tag="N7C74_001726"
     CDS             complement(21329..22420)
                     /gene="leuB"
                     /locus_tag="N7C74_001726"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HGU2431607.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     IELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            complement(22420..23991)
                     /gene="leuA"
                     /locus_tag="N7C74_001727"
     CDS             complement(22420..23991)
                     /gene="leuA"
                     /locus_tag="N7C74_001727"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HGU2431608.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRATEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(24084..24170)
                     /gene="leuL"
                     /locus_tag="N7C74_001728"
     CDS             complement(24084..24170)
                     /gene="leuL"
                     /locus_tag="N7C74_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HGU2431609.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            24828..25772
                     /gene="leuO"
                     /locus_tag="N7C74_001729"
     CDS             24828..25772
                     /gene="leuO"
                     /locus_tag="N7C74_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308107.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HGU2431610.1"
                     /translation="MPEVQTDHPETAEFSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKLNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         5925 a         6851 c         6914 g         6197 t
ORIGIN      
        1 ttgctactaa acaaagtact gctgaacaaa ttactgacaa cgcaattatt gaatgagcca
       61 gaataagcta aggttgaagg ggctggaacg ccccttcaac cttagcagta gcgtgggatg
      121 atttcacaat tagaaagacc tgcatgatga gctagagaag aggctagtga cgcaaggcgt
      181 cgtgtacgac gcggatcacc gagatgggca tcgccaacca gactgctaat tagcccatga
      241 ataacaatca gaaaggacca taacagaccc gataaaatga aatataagag acggtcaacg
      301 ggtgaagaaa aagttcaaaa attcgctgtg gagcaggaag ggaattaccg aatggaaagc
      361 gtagccacac gcaacaactg aaagcagttt ggcagaaaca aaaaatcccc ggactcgggg
      421 atttatgtac aagaggcagc ccttaggatg agggtataaa cgtacaggaa aggttaaaaa
      481 tccgctggcg ctttaaacgt catactattg ccatacgccg gatgggtaat cgtcaacatc
      541 tctgcatgta gcaacaaacg cggtgccatc gctctcgctt ctggtgatgc ataaaaacga
      601 tcgccgagaa tcggatgccc cagcgccagc atatgcacac gcaattgatg cgaacgcccg
      661 gtaatcggtt ttaacaccac tcttgccgtg ttatccgccg catactccac cacttcatat
      721 tccgtctgcg caggtttacc cgtttcgtaa cagactttct gtttcgggcg gtttggccag
      781 tcgcaaatca gcggcagatc caccagacct tctgcggggg atggatgccc ccagacgcgg
      841 gtcacatact gctttttcgg cacgcgctcg cggaactggc gttttaactc ccgctccgcg
      901 gctttggtca gcgccactac aatcacgccg ctggtagcca tatccagacg atgcaccgat
      961 tctgcctgcg gataatctcg ctgaatgcgc gtcatcacgc tgtctttgtg ctcttccaga
     1021 cgacccggca cagacaacaa accgctcggc ttgttgacca ccataatatg gtcatcctga
     1081 tacaggataa ccaaccaggg ttcctgcggt ggattgtagt tttccatccc cattttcggc
     1141 tccgttactg atgcgttaca acgatcaaac gcagggcatc cagacgccaa cctgcctgat
     1201 ccaggctttc cattacctgc tgacggttgc tctcaatggc ggtcagttcg tcgtcacgaa
     1261 tgttcgggtt cactgcacgc agagcttcca gacgagacag ctcggcagac agtttttcgt
     1321 cggcttcgtt acgtgctgca tcaatcaatg cacgggcaga tttctcgatc tgcgcttcac
     1381 ccagttgaag gatagcgtga acatcctgct gcacggcgtt aaccagtttg ctgccggtgt
     1441 gacggttaac cgcgttaagc tggcggttaa aggtttcaaa ctctacctgc gccgccaggt
     1501 tgttgccgtt tttatccagc agcatacgta ccggcgtcgg tggcaggaag cggttgagct
     1561 gcaactgctt cggagcctgg gcttcaacca cataaatcag ttccaccaac agcgtaccta
     1621 ccggcaacgc tttgtttttt aacagtgaaa tcgtgctgct accggtatcg ccagaaagaa
     1681 tcagatccag accgttgcgg atcagcggat gttcccaggt aataaactgc gcatcttcac
     1741 gcgccagcgc cacttcacga tcaaaggtga tggtgatgcc atcttccgac aggccaggga
     1801 agtccggcac cagcatatga tcggacggcg tcagcacgat catgttgtcg ccgcgatcgt
     1861 cctgattgat accgataata tcgaacaggt tcatggcgaa ggcgatcagg ttggtatcgt
     1921 catcctgctc ttcaatgctt tctgccagtg cctgggcttt ttcgccaccg ttggagtgga
     1981 tttccagcag gcggtcacga ccctgttcca gctgtgcttt cagcgcttca tgttgctcgc
     2041 ggcagttttt gatcagatcg tcaaagcctt cggtttgatc cggtgaagcc agatagttaa
     2101 tcagatcgtt gtatacgcta tcgtaaatag tgcgtccggt cgggcaggtg tgctcaaatg
     2161 catccagacc ttcgtgatac cagcgcacca gcaccgactg agcggttttc tccagataag
     2221 gcacatggat ctgaatatcg tgcgcttggc cgatacgatc cagacgacca atacgctgct
     2281 ccagtagatc cgggttgaat ggcaggtcaa acatcaccat gtggctggcg aactggaagt
     2341 tacgtccttc agaaccgatt tctgagcaca gcagtacctg tgcgccggtg tcttcttcgg
     2401 caaaccaggc ggcagcgcgg tcacgttcga taatcgacat accttcgtgg aacaccgcag
     2461 cgcgaatacc ttcacgttcg cgcagtactt gctccagttg cagcgcagtg gcagctttag
     2521 cgcagatcac cagcactttc tgagagcgat ggctggtcag gtagcccatc agccactcaa
     2581 cgcgcggatc gaagttccac caggtggcgt tatcaccttc aaattcctga taaatacgct
     2641 ccgggtagag catatcgcgg gcgcggtctt ccgcactttt acgtgcgccc ataatgccgg
     2701 agactttaat agccgtctga tactgcgtcg gtagcggcag cttaatggtg tgcagctcgc
     2761 gtttcgggaa gcctttcaca ccgttacggg tgttacggaa cagcacgcgg ctggtgccgt
     2821 ggcgatccat cagcatcgaa accagctcct gacgggcgct ctgggcatct tcgctgtcgc
     2881 tgtttgctgc ttgcaacagc ggctcgatat cctgctcgcc gatcatctcg ccaagcatgt
     2941 tcagttcgtc attgctcagt ttgttacctg ccagcagcat ggcaacggcg tccgctactg
     3001 gacgataatt tttctgctct tcaacgaatt gcgcgaaatc gtggaaacgg ttcgggtcca
     3061 gcagacgcag acgggcgaag tggctttcca ttcccagctg ttccggggtt gcggtcagca
     3121 gcagaacgcc cggcacgtgc tctgccagtt gttcaatagc ctgatattcg cggctcggcg
     3181 cgtcttcgct ccacaccagg tgatgcgctt catcgaccac cagcaggtcc cactcggctt
     3241 cacagagatg ttccagacgc tgtttgctac ggcgggcaaa atccagcgag caaatcacca
     3301 gctgttcggt gtcgaacggg ttgtaagcat cgtgctgagc ttcggcataa cgctcatcat
     3361 caaacagcgc aaagcgcagg ttgaaacggc gcagcatttc taccagccac tgatgctgta
     3421 aggtttccgg gacgataatc agcacacgtt cagcagcgcc agagagcagt tgctgatgaa
     3481 ggatcatccc ggcttcaatg gttttcccta aacccacttc gtcagccagc aggacgcgcg
     3541 gcgcgtggcg gcggccaaca tcatgagcga tgttgagctg gtgcgggatc aggctggtac
     3601 gctgaccgcg caggccgctg tacggcatac ggaactgttc gctggaatac ttacgcgcgc
     3661 gataacgcag cgcaaagcgg tccatacggt caatctgccc ggcaaacagg cggtcctgcg
     3721 gtttgctgaa caccagtttg ctatcaagga aaacttcacg cagggctacg ccggactctt
     3781 cagtatccag gcgagtaccg atataggtca gcaagccatt ttcttctttt acttcttcga
     3841 cttgcatctg ccagccgtca tggctggtaa tggtatcacc agggttgaac atcacgcggg
     3901 tcacggggga atcactgcgt gcgtacagac ggttttcacc agtagatggg aaaagtaaag
     3961 tgacagttcg cgcatccacc gcgacaacgg ttccaagtcc caattcgctt tctgtatcgc
     4021 tgatccagcg ttgaccaagt gtaaaaggca tatgtgttcg gctctatatc tttaattgca
     4081 ggcaataacc acccgctacc gtgcttatga ggtagtggtg ttattcaggt ccaggaatgg
     4141 aaagggcgct atggtactgg atggcaaagc attcgtcacg catcaaaatg gtatctggcg
     4201 aactcttttt ttgctcaaaa tagcccaagt tgcccggtca taagtgtagc aaaattatcc
     4261 tcaataaaag ggagtattcc ctccgccacg ggttgtagct ggcgggtcag atagtgttcg
     4321 taatccagtg gtgaacgttg gtagtccagc ggctccgggc cgttggtggt ccatacgtac
     4381 ttaatggtgc cgcggttctg atattgcaag gggcgaccgc gcttttggtt ttcttcatcg
     4441 gcaagacgag cggcgcgtac atgaggcgga acattacgct gatattcgct cagcggacgg
     4501 cgaaggcgtt tacggtaaac cagccgcgca tccagttcac ccgccatcag tttgtcgatg
     4561 gtttcgcgga agtactcctg atatggctcg ttgcggaaga tacgcaggta aagctcctgc
     4621 tgaaactgct gggccagcgg cgtccagtcc gtacgcacgg tttccagccc tttaaacacc
     4681 atccgctgtt tgtcgccctc ctgaatcagc ccggcataac gctttttact gccggtatcg
     4741 gctccgcgaa tggttggcat cagaaaacgg cagaaatggg tttcatactc cagttccagt
     4801 gcgctggtca gctgttgttt ttgcagcgtt tctgcccacc agacgttaac gtgctgcacc
     4861 agtgcacgac cgattttcgt cgcttcttct tccgaatgtg cgcctttcag ccagacaaac
     4921 gttgagtcgg tatcgccgta gatcacgtcg tagccctgcg cttcaatcaa cgctttggtt
     4981 tgccgcatga tctgatgacc acgcatggtg atcgacgata ccagccgcgg atcgaagaag
     5041 cggcaggcgg tggtgccgag cacgccataa aaggcattca tgatgatttt cagcgcctgc
     5101 gacagcggtt tgttaccctg gcgtttggct tcatcgcgtc cgtgccagat gttagtcaca
     5161 atctccggca ggcaatgttt ttctcgcgag aaccaggcat cgagaaagcc ttcggtgctg
     5221 tgctctggat caggctgcgc catgccttcc accagcccga cgggatcaat cagaaaggtg
     5281 cggatgatcg acgggtacag gcttttgtag tccagcacca gcaccgaatc ataaagccca
     5341 ggtcgcgaat ccatcacgta gccgccaggg ctggcgtgcg gcggcacttc gccgagatta
     5401 ggcgcgacat aaccagcgcg atgcatacgg ggaaaataga gatgaccaaa cgctgccacc
     5461 gacccgccgt gtcgatccac cggcaggccg ttcaccgttg ctcgttcgag caaaaacggc
     5521 atgatttcag ttttatggaa gatctgcgtc accagctcgc aatctttcag gttataagtt
     5581 gccagcgcag gtttatcttc ggcgaaacgg cggtcaattt cgtccattcg atcccacggg
     5641 ttatcgatag attttccttc gcctaatagc tcctgagcga cagtttccag cgagaatgaa
     5701 gagaaattcc agaacgcgga tttcagcgcc tcgataccgt cgataattag ccgaccttta
     5761 gcctgggcaa aaaagacgcc gtttttaaag ccgtgctcgc gccactccag ctcactgtta
     5821 tcgcgcccca gccgcagcgg aatacgataa cgctcggcat gtttttgcag cattcgcaga
     5881 tcaaactgca ccacgttcca accgatgatc acatcaggat cgtagtttgc aaaccaggcg
     5941 ttgagttttt ccagtagctg cgggcggctg gcgacgtact ccagttcaaa atcgagcgcg
     6001 gaggcgtcgc cattctctgg tcccagcata taaacgatgc gctgcccgca gccctccagg
     6061 ccgatgcagt acagctctcc gtggcgggtg gtttcaatat caatagaaac ccacttgagc
     6121 ggcggacgat agtcgggatg cggtttcaga cgggcattaa cgatagcgcc attgtgcata
     6181 tcaccctcga cccacaccgg tgaggtgata aaccgctcca tcagatagcg ttctggcggg
     6241 cgcacatcgg cctcgtagac ggtaacgcca ccttcacgca ggcgcttttc gtaattcatc
     6301 aactggcgat gggcgcgaca gtaaaggcca tacaccggct ggcggtgaaa atcctttaac
     6361 gccagcggcg tcaggcgaaa gccttgttca ccccgcaaaa tatgctgagc gcggggaacc
     6421 tgatcggcgg gaataaacgc cacggactct tgcggtgcaa gcgtaacctg caacggcccg
     6481 ttgtccgtcg ccagccagaa ggagacctct gttccttgcg gggtatcccg ccagtgtcgg
     6541 gttaagataa aacctgcctg cgccacgctg aaaatccatc aaaaaaccag gcttgattat
     6601 agcctggttt catttgattg gctgtggttt tatacagtca ttactgcccg taatatgcct
     6661 tcgcgccatg cttacgcaga tagtgtttat ccagcagcgt ttgctgcata tccggtaact
     6721 gcggcgctaa ctgacggcag aatatcccca tataagcgac ctcttccagc acgatggcgt
     6781 tatgcaccgc atcttcggca tttttgcccc atgcaaatgg gccgtgagaa tggaccagaa
     6841 cgccgggcat ttgcgctgca tcgataccct gtttttcgaa ggtttctacg atgacgttac
     6901 cggtttccca ctcatattcg ccgttgattt ctgcgtcggt cattttgcgg gtgcagggaa
     6961 tggtgccgta gaaatagtcg gcgtgggtgg tgccggttgc tggaatcgac tgacccgcct
     7021 gcgcccagat ggtggcgtgg cgcgagtgcg tgtgcacaat gccgccaatg gacgggaatg
     7081 cctgatagag tagtcggtga gttggcgtat cggaggaggg ctttttcgca ccttcaacca
     7141 cttcaccggt ttcgatgctg accacgacca tatcgtcagc ggtcatgatg ctgtaatcga
     7201 cgccggaagg tttgatcaca aagacgccgc gctcgcgatc aacggcgctg acgttgcccc
     7261 atgtgagcgt gaccaggttg tgttttggca gcgccaggtt ggcttctaat acctgacgtt
     7321 tgagatcttc taacatgttg actccttcgt gccggatgcg cgttgcttat ccggcctaca
     7381 aaatcgcagc gtgtaggcca gataagacgc gtcagcgtcg catcaggcgc tgaatgccgg
     7441 atgcgcgttg cttatccggc ctacaaaatt gcagcgtgta ggcctgataa gacgcgccag
     7501 cgtcgcatca ggcgttacat accggatgcg gctacttagc ggcgaaaccc gtaatacact
     7561 tcgttccagc gcagcgcgtc tttaaacgct ggcaggcggg tgtcgttatc aatcaccgtg
     7621 atttcaatgt cgtgcatctc ggcgaactgg cgcatatcgt tgaggttcag cgcatggctg
     7681 aagacggtat ggtgcgcgcc accagcgagg atccacgctt cggaagcagt tggcagatcc
     7741 ggttgcgctt tccacagcgc attcgccacc ggcagtttcg gcagggagtg cggtgttttc
     7801 accgtgtcga tgcagttaac cagtagacgg taacgatcgc cgagatcaat caagctggcg
     7861 acaatcgctg gaccggtttg ggtattgaag atcaggcggg caggatcgtc cttaccacca
     7921 ataccgagat gctgaacgtc gaggatcggt ttctcttctg cggcgatcga cgggcagact
     7981 tccagcatat gggagccgag caccaggtca ttgcctttct cgaagtgata ggtgtagtcc
     8041 tccataaagg aggtgccgcc ctgcagaccg gttgacatca ccttcatgat gcgaagcagg
     8101 gcggcagttt tccagtcgcc ttcgcccgca aagccgtaac cctgctgcat cagacgctgt
     8161 acggccagac caggaagctg tttcagaccg tgcaaatctt caaaggtggt ggtgaacgcg
     8221 tggaagccac cttgttccag gaaacgtttc atccccagct caatacgcgc cgcttccagc
     8281 acgttctgtc gtttttcgcc gtggatttgt gtggcaggcg tcatggtgta gcagctttcg
     8341 tactcatcga ccagcgcgtt aacatcgccg tcgctgatgg agttcaccac ctgcaccaga
     8401 tcgccaaccg cccaggtatt gacggagaaa ccgaacttga tctgtgcggc aactttatcg
     8461 ccatcggtga ccgccacttc acgcatgtta tcgccaaatc ggcagacttt cagatgacgg
     8521 gtatcctgtt tagataccgc ctgacgcatc caggagccga tacgctcatg ggcttgttta
     8581 tcctgccagt gaccggtaac cacggcatgt tgctgacgca tacgcgcgcc aatgaagccg
     8641 aactcgcgac cgccgtgcgc ggtctggttc aggttcataa agtccatatc gatgctgtcc
     8701 cacggcagcg ccgcgttgaa ctgggtgtgg aattgcagca acggtttgtt gagcatggtc
     8761 aggccgttga tccacatttt ggccggggag aaggtgtgta gccacaccac cagaccagcg
     8821 caacgatcgt cgtaattagc atcacggcaa atagcggtga tttcatccgg cgtggtgccc
     8881 agcggtttca gtaccagttt gcagggcagt ttcgcttccg tattcagcgc tttcacaacg
     8941 tgctcggcat gttgggtgac ctgacgcagg gtttccgggc catacagatg ctggctgcca
     9001 attacaaacc acacttcata attatcaaaa atcgtcatta tcgtgtcctt atagagtcgg
     9061 aacggcctgg gcagcctgtg ccggggcgga agttggaaga tagtgttgtt cggcgctcat
     9121 cgcccattgc tgatagcggc gataaagctg ttcaaagcgt tgtgcctgtt cgctgcgcgg
     9181 ttgcagggtt ttctctaccg cactggccat tttttgctgg gctgatggga tgtctgcgtg
     9241 cactttcgcg gcgacggcag caaaaatcgc cgcaccgagc gcacagcatt ggtcagaggc
     9301 aacaatttgc agcgggcgat tcagcacgtc gcagcaggcc tgcataatga cctggttttt
     9361 ccgcgcgatg ccgcccagcg ccatcacgtt attgacggcg atcccctgat cggtaaagca
     9421 ctccatgatt gcgcgtgcgc caaaggcggt ggcagcaatc aaaccgccga acagcagcgg
     9481 agcgtcggta gcgaggttaa gatcggtaat cacccctttc aggcgttggt tagcgttcgg
     9541 tgtgcggcgg ccgttaaacc agtcgagcac caccggcagg tgatccagag acggattttt
     9601 ggcccatgct tcggtcagcg ccggaagcag ttgtttctgg ctggcgtcga tttgctcttt
     9661 tagttccgga tgctgggcgg caagctgttc cagcggccag ccgagtacgc gaccgaacca
     9721 ggcgtagata tcaccaaacg ccgattggcc tgcttccaga ccgataaatc caggcaccac
     9781 gctgccatca acctgaccgc aaataccttt cactgcccgc tcgccaacgc tctgtttgtc
     9841 ggcaatcaga atgtcgcagg tggaagtacc gataactttt accagtgcgt taggctgtgc
     9901 gcctgcacca actgcgccca tatggcagtc aaacgcgccg ccggaaatca ccacgctttc
     9961 aggcaggccg agacgctgcg cccattccgg gcataaggtg cccaccggaa tatcggcagt
    10021 ccaggtatca gtgaacagcg gggaaggcaa atggcgattg aggatcggat ccagctcatc
    10081 aaagaaactg gctggcggca ggccgcccca gctttcgtgc catagcgact tatgcccggc
    10141 gctgcaacgt ccgcgacgaa tatcctgcgg gcgcgtggta ccggaaagca gagctggcac
    10201 ccagtcgcac agctcaatcc acgatgcggc agattgcgcc acggcgctgt cctggcgagt
    10261 cacatgcagg atttttgccc agaaccattc gctggaataa ataccgccaa tatagcggga
    10321 gtagtcaaca ttgcccggcg cgtggcacaa acgggtaatc tcttccgctt cttcaaccgc
    10381 agtgtggtct ttccacaata cgaacatcgc gttcgggttt tcggcaaact ccgggcgcag
    10441 cgccagcacg ttaccgtcgg catcaatcgg tgcgggcgtc gagccggtac tgtcaacgcc
    10501 aatcccgacc acagctgcgc gctgttcgac gctaagttct gcaagcacgg ttttcagtgc
    10561 cgcttccatt gactcaatgt agtcacgcgg atgatgacgg aactggttat tcggggcatc
    10621 acaaaattgc cctttttgcc aacggggata ccactctacg ctggtggcga tttcttcacc
    10681 ggtggtgcag tccaccgcca aagctcgcac agaatcactg ccaaaatcga ggccaattgc
    10741 aatcgccatc gtttcactcc atccaaaaaa acgggtatgg agaaacagta gagagttgcg
    10801 ataaaaagcg tcaggtagga tccgctaatc ttatggataa aaatgctatg gcatagcaaa
    10861 gtgtgacgcc gtgcaaatta tcaatgtgga cttttctgcc gtgattatag acacttttgt
    10921 tacgcgtttt tgtcatggct ttggtcccgc tttgttacag aatgctttta ataagcgggg
    10981 ttaccggttg ggttagcgag aagagccagt aaaagacgca gtgacggcaa tatctgatgc
    11041 aatatggaca attggtttct tctctgaatg gcgggagtat gaaaagtatg gctgaagcgc
    11101 aaaatgatcc cctgctgccg ggatactcgt ttaacgccca tctggtggcg ggtttaacgc
    11161 cgattgaggc caatggttat ctcgattttt ttatcgaccg accgctggga atgaaaggtt
    11221 atattctcaa tctcaccatt cgcggtcagg gggtggtgaa aaatcaggga cgagaatttg
    11281 tctgccgacc gggtgatatt ttgctgttcc cgccaggaga gattcatcac tacggtcgtc
    11341 atccggaggc tcgcgaatgg tatcaccagt gggtttactt tcgtccgcgc gcctactggc
    11401 atgaatggct taactggccg tcaatatttg ccaatacggg tttctttcgc ccggatgaag
    11461 cgcaccagcc gcatttcagc gacctgtttg ggcaaatcat taacgccggg caaggggaag
    11521 ggcgctattc ggagctgctg gcgataaatc tgcttgagca attgttactg cggcgcatgg
    11581 aagcgattaa cgagtcgctc catccaccga tggataatcg ggtacgcgag gcttgtcagt
    11641 acatcagcga tcacctggca gacagcaatt ttgatatcgc cagcgtcgca cagcatgttt
    11701 gcctgtcgcc gtcgcgtctg tcacatcttt tccgccagca gttagggatt agcgtcttaa
    11761 gctggcgcga ggaccaacgc atcagccagg cgaagctgct tttgagcact acccggatgc
    11821 ctatcgccac cgtcggtcgc aatgttggtt ttgacgatca actctatttc tcgcgagtat
    11881 ttaaaaaatg caccggggcc agcccgagcg agttccgtgc cggttgtgaa gaaaaagtga
    11941 atgatgtagc cgtcaagttg tcataattgg taacgaatca gacaattgac ggcttgacgg
    12001 agtagcatag ggtttgcaga atccctgctt cgtccatttg acaggcacat tatgcaagca
    12061 ttgctggaac actttattac ccaatccacc gtgtattcat tgatggcggt ggtgttggtg
    12121 gcctttctgg agtcgctggc gctggtcggt ttgattctac ccggtacggt gctgatggcg
    12181 gggctgggag cgctgattgg cagcggcgag ttaagtttct ggcacgcctg gctggcaggg
    12241 atcgtcggtt gtctgctggg cgactggatc tccttctggc tgggctggcg ttttaaaaag
    12301 ccattgcatc gttggtcatt tctgaagaag aacaaagcat tactcgataa aactgaacat
    12361 gcgttgcatc aacacagcat gttcaccatt ctggtcggtc gttttgttgg cccgacgcgt
    12421 ccgctggtgc caatggtggc gggaatgctg gatctcccgg tggcaaaatt tatcacgccg
    12481 aatattatcg gctgcctgct gtggccaccg ttttacttcc tgccggggat tctggcgggt
    12541 gcggcgatcg atattccagc cggaatgcag agcggtgagt ttaaatggtt gctgctggcg
    12601 acggcggtat ttttgtgggt tggtggctgg ctgtgctggc ggttatggcg cagcggcaaa
    12661 gcaaccgatc gtttgagtca ttatttgtcc cgcggtcgtt tgttgtggct gacgccgttg
    12721 atttctgcca tcggcgtggt ggcgctggtg gtgttagttc gccatccgtt aatgccggtg
    12781 tatatcgata ttttgcgtaa agtggttgga gtttagggca tagtcttgtg tggggcgctt
    12841 gatgcggaca aaacgcaccc gtaggtcgga taaggcgttc acgccgcatc cgacagtgca
    12901 tattaacctg taattcccaa cagcgccgaa gcactcgcct taccgctcaa caactcattg
    12961 gtcataccct gccaggcgat tcgcccgtcg gcgactacta ccgagcgcgt ggcaatccgc
    13021 gccgcatctt ccacactgtg cgacaccatc aatagcgtca ttttttgctg ctggcagcta
    13081 gtgctcacca gcgtcaacat ctcctgacgt agcgccggat cgagcgcaga gaacggttca
    13141 tcgagcaata aaatcggctg ttcgcgtacc agacaacgcg ctaacgccac gcgctgtcgc
    13201 tgaccgccgg aaagctcgcc cggtaaccgc gccattaaat tatcaatccc catctgacgg
    13261 gcaatcgcgt gcattttctc ctgctgtacc gcgttcagct tcaatcctgg attaagcccc
    13321 agcccgatgt tctgcgcgac cgtcaggtgg ctgaacaggt tgttctcctg aaacagcatc
    13381 gacaccggac ggcgtgacgg cggcgtcgtg gtgtgatcaa cgccgtcgat agtcagcgca
    13441 ccgctggctg gtgtcagaaa accggcgatc aagttcagca gggtactttt acccgcgccg
    13501 cttggcccga ggatcgccac ctgctcgccg cgttctaccg ttaagctaaa acgcatcggc
    13561 aaatggtgat aaagccaggt gatatcagtc agttttaaca tttcgccccg gtattttttc
    13621 aatcacgcta aacagcagaa aacagagcag cagcagaatt aacgcggtga ccgcgccgtc
    13681 ctggctgcga taggagccaa tttgctggta gagataaaac ggcagggtgc ggaaatcatc
    13741 gttaccgaac aacgccacca cgccaaaatc accaatcgac agcacgcagg caaaggccag
    13801 cgcctgcgcc agcgggcgtt tcagggcgcg cagctccact acttttaagc gtgaccagcc
    13861 ttcaatcccc agcgactgac ataacatgct gtagcgggcg gtgatatcgc gcatcgggtt
    13921 ttccagcact ttcagggcgt aggggatcgc cattaacgca ttggtgaaaa tcacaatgcc
    13981 gtcagcagat tgtggcaggc cgatagtgtt gttgagcagt aaaaagaagc ccgttgccag
    14041 cacaatcccc ggcatagcga ggatcaacat gccgctcatc tccagcgcct ggcccgccag
    14101 cattttttgc cgcgcccgca gttcgcgact gctccatagc agcatcatgg tcagcactac
    14161 gcacaataca cctgccgcca gcgcaatacg caacgaggtc cacagcgcct gccacagcac
    14221 cggttgtgcc agcacttccg gcaactggcg atttacccca tcgacgatca ccgccagcaa
    14281 cggtggcagc aacagcagca gcgccagcac aattaacatc gtgtcgcaaa tgcggctatg
    14341 cagacgatcg tccgggtcgc gccagccttg cagcagcgtg gtgccgggcg caatggcctt
    14401 actcagtcgc tgactcaaca gcaccagccc aaggcaacac accatctgga tcagcgccag
    14461 catcgccgcg cgggcaggat cgtagtcgta actcagcgcc tgatagattg ccagctcgat
    14521 agtggttgcc tgcggaccgc cgcccagcga taacacggtg gcgaagctgg cgaaacagag
    14581 cataaagata agcgcggtaa ccggcgggat ttgtcgccgt aaccacggcc attcgacgaa
    14641 gcggaaaaaa tgccagccgc gcatcccaag ctgggcggca agctgacgtt gttcgccggg
    14701 aatgttttcc agtgcctgga gtaataagcg gctcgccatc ggcagattaa aaaacacatg
    14761 ggccagcaaa atgccttgca ggccgtaggg cgaaaaggtc cactccagac cgagcgattg
    14821 gcagagcgat gccagccagc cctggcgacc atagacgcta agaatgccga aaacagcaac
    14881 cagcaccggg aggatcaagg tcattgcgca cagacgcaac agcgccagcc gacccggaaa
    14941 gcgcctgcga tagagcgcgc gggcgaggaa tatcgagggt acgacagaga gtagcgccga
    15001 gagaaacgcc tgccagaagg agaagcgcac cacatgccac aggtagctgt cctgccagac
    15061 tgccgaccag tcaccctgcg gcgcgttcca ccacagggcg agaaacgccg ccagcgcaac
    15121 cgctaccacc agcgtggcgg cgcttacacc tggaattaac cagccgggaa ttaacggctg
    15181 acggcgcgtt gccattcgct aatccatgcc tgacgttgtg ccgccacttc ggctggcgtg
    15241 aactccagcg tggttgcggg tttggtcaat ttttcaaaac cggcaggcag cgtgacgttt
    15301 gccaccggat acatccagtt gccggttggg atcgcattct ggaaagccgg agaaaccata
    15361 aactggagga atttttgcgc cagctccggc tgcttgctgg cagcggtgcg agcggcgact
    15421 tccacttgca gatagtgacc ttcgctgaag ttcgcggcgg cgtagttatc tttcttctct
    15481 tcgagaatgt gataagccgg agaggtggtg taactcagta ccagatcgct ttcacctttt
    15541 aaaaacaggc cgtaggcttc gctccagcct ttggtgaccg tgaccgtttt cttcgccagt
    15601 ttctgccagg cttgtggggc gtcatcgcca tagacttttt gcatccatag caacagaccc
    15661 agccccggtg tactggtgcg cggatcctga taaatcaccc gccagttttg atcgctctca
    15721 accagttctt tcaggctttg tggcgggttt ttcagtttgt tcttgtcata aacgaaggcg
    15781 aagtagccat aatcaaacgg tacgaaaatg tcattattcc agccgccggg aacgttaacg
    15841 gcatccgctg ccacaccgct tttggcaaac agtccggttt tactggcggc gtctaacaga
    15901 ttgttatcca gccccagcac cacatcggct ttactgtttt tgccttccat ccgtagacgg
    15961 ttgagaagcg aaacgccatc ttccagcgcc accagtttca gttcgcaatt acagtcggct
    16021 tcaaaggctt ttttaaccac cggaccaggc ccccagtcgg cggcgaagga atcgtaggta
    16081 taaacagtca gaacgggttt agcgaaaacg ggcgctgtgc acagcaacag caggggaaga
    16141 cattttttta acactttgca cctcaaaaaa gagtggcaaa ggacttgaga aggagcctca
    16201 aatcccttcg ccggcgttat ccggatcagg ttcgacgggt attttctcag cgcacgcgtc
    16261 cgcgtggcac cccgttgaga acggcgttag tgtagtgatt ttgttatcaa ccagcaatcc
    16321 atagagaagg cgtattaaaa aagccaatat tcgttcagct atcaagagcg tatttgcata
    16381 gctggagagc gttgatccga aagaatccct tcgctacagt caaaataggt aacgtaaccg
    16441 tttattgcgt gaatgagcga tttttgacag tattcttatt caggacgctt attctgagtt
    16501 ttttattcag agtggagcta tcagcatgaa agtaagaaac ccagaacaga ttagtatccc
    16561 tgctagtaat accactaaag atccaggcct tactaattct cagattatca ggatggctaa
    16621 cttggtcaaa aagaccgaga atatgaatat ctttgaagag ctatgggaga cgttacgcaa
    16681 tctcttccag tcagataaac attcccagac tgcggcaagg caaattctga aggatgcatt
    16741 ttatttccag aatagtgatg actactcaaa atactttacc ggggctgtcg acgggaaggc
    16801 gcgtgataag ttaactcact ggctgataaa atttaatgaa ctcaaagaat acgcaaaaga
    16861 tccggaaaat atggccgcga aggcctcact ctcgccagaa ggtacgttat gcgtcagttt
    16921 ttttattggc gatgaggcga tatttactct ggagttgcag ctgaaaaaga gcaccaggac
    16981 cggggggatt gatctgagta atgcttactt taacggggtt gtcatttgtg gtattgactg
    17041 tctcgaggtt gacctgagca atgctgaaac gaacaatagc cgttggtatg actgacctgc
    17101 gcgaggttaa cctgaaaaag ctgacctttc cggccagaac aaggctagac tgcgccgtta
    17161 ttgattaact cagaatatga aagaggggct ggaatgcccc ttctttaatc ttatttgcga
    17221 cgctaatcag acgatgcata aaacagagaa aatataaaag taatgtattt tatcattccg
    17281 tctgaatata aaaaatgcgc tcatgaccgc caaaaggcgc aagctactct ggatccggcg
    17341 gcgcaaacca cgctgattta aaatcaaacc agccgagggt gttcatgcgc aggccgcgca
    17401 tactgcgttg tccctgaatg atcagccagt ggtggataag tggcaccatt gctttgctgg
    17461 cgaccagttg ttggcaccag ttcgccaggt tcatttcgcc attgcgccag cgggcggcgt
    17521 cgacttgcca gtcgattgga atacagtgtt ggagcagcgg tacctcgcac aggtgcgcga
    17581 acagcgaaaa atccagcggc agcgtaaagt tggcgctgtt aagccagata tcgctctcga
    17641 tctctccttc atgccactga tcgtagctga tctctttgat ttccagtgtg acctggtggc
    17701 ttgccagaat ctgctgcatg atcccggcaa tcacccgatg ctcactgtga tcctgataaa
    17761 aggtcagggt gaggctttcc agaccagccg gtttttcgct cgttatggtg cgggcatgat
    17821 gccaacgggg gagcagtcca taagccggga accacagttg ctggtactgt tcctcagcga
    17881 aatagaccag attagttgga gaaagcacat agcttaccca gtccctgact tgctgattcg
    17941 ccccgcgatg ggtgcggcta tcgaacagta aatagtagca accttcctcc aggcggcttt
    18001 caatctcttt ttcctcgccc tgtggacctt ttagcatcag ccctccggct ggctcgtcgg
    18061 caatttccgg cagaacccag acgttaactt catcgattaa tgcccggtaa ccgaagaagt
    18121 catcgaatgc ctgaattttc agttgattgg tgctgttgcg aatcaccgca tacggaccgg
    18181 tgccgatggg atggctggca aagttactga gggtttccca ttcgcgcggc aggatcatcg
    18241 ccggaacttg tcccagcagt aacggcaacc agcgatccgg ctgcgtgagg tggatatcca
    18301 gcgtccaggg cgtcggcgac acaatgtcag caatatgcga atagagcggc agcgtattga
    18361 ttcgttttaa agaggcgatc acgtcgtcca tttccagttc acgaccatga tgaaaatgga
    18421 ctcctggacg caaaaagaaa cgccagtgaa gcggtgatat ttgctgccag tggtgggcga
    18481 tgtctgcttc cagttcccca ttttcctcat ttatgcgcgt tagcgaactg aagatttgcc
    18541 gggcgatatg ggtttcggaa cggcgcaatg cgctgccagg tagcagatta cgcaacggac
    18601 gatagtagag cacgcgcagg atgtgccgcc cctggcggaa gctgcggccc agatgagaaa
    18661 ccagcatttg cctcacagtc gctttgtcgc caactaactg cactagttgg tcgatacggt
    18721 cctgctccag caggtcttcc gcccgctgtt gctgaagcgc cagcccggta tagaggaatg
    18781 tcagacgcga gcgtttacca cgcccgactt ccgcttccca cgtcagccag ccgcgatcct
    18841 gcatggtgtt gagcagggta cgcatatgac gacgcgagca gctcaataac gctgccagtt
    18901 cgttgagcgt tgtgtcctgc gatttaccct cgcagcattg ccacaggcgg atgaactgtt
    18961 gttgcagacg agcagatggc ataaaagggg aactcctgcg caaaagacag caattttatt
    19021 ttccctatat taagtcaata attcctaacg atgaagcaag gaggtgcccc attcgtcagt
    19081 tttatcagca ctattttacc gcgacagcga agttgtgctg gttgcgttgg ttaagcgtcc
    19141 cacaacgatt aaccatgctt gaagaactga tgcagtggga ggggagtcat tctgactact
    19201 gatttgcgga catcatgtgt gactgagtat tggtgttaat cgccacgcca gcagtgatta
    19261 tctgctggct tttttctttt tggcgattga gtatcggacg gtgtacaaac taacgcataa
    19321 acgcgggttg cttcgcttca taagccgcaa tggcgtcgtc gtgctgcaag gtaagcccaa
    19381 tgctgtccag accgttcatc atgcagtggc ggcggaaggc atcaatggta aagcgatagg
    19441 ttttctcacc cgctttcacc tcttgcgctt ccaggtctac atcaaaatga atccccggat
    19501 tggctttcac cagcgcaaac agctcatcca cttctgcgtc gcttaatttc accggcagca
    19561 gctggttgtt aaagctattg ccgtagaaga tgtcagcaaa actcggcgca atcaccacct
    19621 taaaaccgta gtcggtcaat gcccagggcg cgtgctcacg cgaggagcca cagccgaagt
    19681 tttctcgtgc cagcaaaatg gaagcgccct gatactgcga gaagttcagc acgaagtccg
    19741 ggtttggctg ttggcctttt tcatccagaa aacgccagtc gttaaacaga tgcgcgccaa
    19801 aaccagtacg ggtcactttc tgcaaaaact gtttcgggat gattgcatcg gtatcgacat
    19861 tggcggcatc cagcggaacc accaggcctg tgtgtttgat aaatttctct gccatggtgt
    19921 gctccttatt taatgttgcg aatgtcggcg aaatggccgg tcacagccgc ggcggcagcc
    19981 attgccgggc tgaccagatg cgtgcgcccg ccgcgcccct gacggccttc aaagttacgg
    20041 ttgctggtgg aggcacaacg ttcgcccgga ttcagacggt cgttgttcat cgccagacac
    20101 attgagcagc caggcaagcg ccattcaaaa ccggcttcaa taaagatttt atccagacct
    20161 tctgcttccg cctgcgcttt taccggacca gagccgggaa ccaccagcgc ctgtacgcct
    20221 ggcgcgactt tccgcccttt ggcgatttcc gccgccgcgc gtaaatcttc aatacgtgag
    20281 ttggtgcagg aaccgataaa cactttgtcg atagccactt cggtcagcag aatacccggt
    20341 ttcagcccca tataggccaa cgctttttct gccgacgcgc gttcaaccgg atcggcaaac
    20401 gaagccggat cgggaatatt gtcgttcacg gaaatcacct ggcctgggtt agttccccag
    20461 gtgacctgcg gtgaaatctc ttctgcctgc aaagtgacaa cggtatcgaa agttgcgcct
    20521 tcgtcggttt gcagggtttt ccagtaggca acggcgtcgt cgaaatcttt gcctttcggc
    20581 gcatgcagac ggcctttgac atagttaaag gtggtttcgt caggtgcaac cagaccggct
    20641 ttcgcgccca tttcgattgc catattgcac agggtcatcc gaccttccat gcttaaatca
    20701 cggattgctt cgccgcaaaa ctccaccaca tgcccggtgc cgcctgcgct accggtttta
    20761 ccgataattg ccaacacgat atcttttgcg gtaatgcccg gcgcggcttt gccctggact
    20821 tcaattttca tggtcttcgc acggccctgt ttcagggttt gcgttgccag tacgtgttca
    20881 acttcggaag tgccgatacc aaaggccagt gcgccaaacg cgccgtgggt ggcggtatgc
    20941 gagtcgccgc agacaatggt catccccggc aaggtcacgc cctgttccgg ccccattacg
    21001 tggacgatcc cttgataggg gtgattcagg tcatacagtt cgacgccaaa ttctttgcag
    21061 tttttgatca gctcctgcat ctggatgcgc gccatttcac cgcaggcatt aatgtcttta
    21121 gtctgggtag agacgttgtg atccatggtg gcgaaggttt tgcccggctg acgtaccggg
    21181 cgaccgtggg cgcgcagacc atcgaacgcc tgcggtgagg tcacttcatg caccaggtgg
    21241 cggtcgatat ataacagcgg ggtttcattt tcggcttcgt acactacgtg agcgtcgaat
    21301 aatttttcgt ataacgtctt agccatgatt acaccccttc tgccacatag cgggcaatga
    21361 tatcgcccat ttcatcggta ctaacggcgg cagcgccacg ggctaaatcc ccggtgcgaa
    21421 tgccttcttc taatgcgcgg ttaatggcgc gttcaatggc ggaagccgca tcatcggcat
    21481 ccaggctgta acgcagcagc agtgccagcg acagaatttg cgcaatcggg ttggcgatgt
    21541 ttttacctgc gatatctggt gccgagccgc ccgccggttc atacagtcca aaaccttgct
    21601 cgttcaggct ggcggaaggc aacatcccca tcgagccagt gatcattgcg cactcgtcag
    21661 acagaatgtc gccaaacagg ttggagcaca gcagaacgtc aaactgtgat ggatctttaa
    21721 tcagctgcat ggtggcgttg tcgatgtaca tatgcgccag ttcgatatcc gggtattccg
    21781 tggcgatctc gttgacgatt tcacgccata aaatagagga ttgcagcacg ttggctttat
    21841 cgatcgacgt cactttatgg cgacgtttac gagcagattc aaacgcgatg cgggcaatac
    21901 gctcgatctc aaaacggtga tacacctcgg tatcaaacgc tttttcatat tgtccgctac
    21961 cttcgcggcc ttttggctga ccgaaataga tgccgccggt cagttcgcgc acgcacagga
    22021 tgtcgaagcc gtttgcggca atgtctgcac gcagcggaca gaatgcttcc agcccctgat
    22081 acagttttgc cggacgcagg ttgctgaata atttgaagtg cttacgcaaa ggcaacagcg
    22141 cgccgcgttc tggttgctgg tccggtggta aatgttccca tttcgggccg cctaccgagc
    22201 caaacagcac ggcatcggct tgctcacaac cttcaaccgt cgcaggcggc agtggttgcc
    22261 cgtggttatc aatggctgcg ccgcctacat cgtaatggct ggtagtgatg cgcatcgcaa
    22321 agcggttgcg cacggcatcc agcactttca gcgcctgggt catcacttcc ggaccaatgc
    22381 catcccccgg caatacggca atatggtaat tcttcgacat cacacggttt ccttgttgtt
    22441 ttcgttgtgt tgagctttgc gttgcaactc tttttcgact tctgtggcac gccagatatt
    22501 gttaagtacg tgcaccatgg ctttggcgga ggactcgaca atatcggtgg ccaggccgac
    22561 gccgtggaag cggcgaccgt tgtagttggc gacaatatcc acctgaccca gcgcatcttt
    22621 accgtgacct ttggcggtca ggctgtattt caccagttcg acgttatagt cagtgatgcg
    22681 gttaatcgcc tgatagacgg catcgaccgg accgttaccg ttggcggctt ctgctttgac
    22741 ttcttcgccg caggccagtt tgacggcggc ggtggcgata tcgttagagc cagactgcac
    22801 gctgaagtaa tccagacgga aatgctccgg ctcttcttgc tgcttaccga tgaaggccag
    22861 cgcctccaga tcgtaatcaa acacctgacc ttttttgtcc gccagcttga ggaaggcgtc
    22921 gtacaaattg tctaaattat attcactttc tttatacccc atctcatcca tgcgatgttt
    22981 caccgccgca cgccccgaac gagaggtcag attcagctgg atttggttca gaccaataga
    23041 ttctggtgtc atgatttcgt agttttcgcg gtttttcagc acgccatcct ggtggatacc
    23101 ggaggagtgt gcgaatgcgc cgctgccaac aatggctttg tttgccggga tcggcatatt
    23161 acaaatctgg ctaactaact ggctggtgcg ccatatctcc tggtgattaa tggcggtgtg
    23221 gacgttgaga atatccttac gaactttaat cgccatgatg acttcttcca gggaacagtt
    23281 accggcacgc tcgccgatcc cgttcattgc accttccacc tggcgtgcac cggcatgtac
    23341 cgccgccagt gagttgccga ccgccaggcc caaatcgtcg tgggtatgta cggaaataat
    23401 ggctttgtcg atgttaggca cgcgttcata caggccgcta atgattccgg cgaactcaaa
    23461 cggcatggtg tagcccacgg tgtccggaat gttgatggtg gtggcaccgg cattaatcgc
    23521 cgcttcgacc actcgcgcca gatcggcaat gggtgtacgc ccggcatctt cgcaagaaaa
    23581 ttcaacatca tcggtgtaat tacgggcgcg tttcaccata tagatagcgc gttcgatcac
    23641 ctcgtccagc gtgctgcgca gcttggtggc gatgtgcatt ggcgaagtgg caataaaggt
    23701 atgaatacgg aaggcttcgg cgactttcag ggattcggcc gccacgtcga tatctttctc
    23761 cacgcagcga gctaacgcgc atacacggct gtttttaacc tggcgagcga tggtttgcac
    23821 tgattcaaaa tcacccggcg aagagacggg gaacccgact tccatcacgt caacacccat
    23881 acgctcaagg gccagcgcaa tttgcagttt ttctttcaca ctcaagcttg cctgtaacgc
    23941 ctgttcaccg tcgcgcaatg tggtatcgaa aataatgact tgctggctca tggtttgggt
    24001 ccttgtctct tttagagcgc ctcgcttcgg gcataaaaaa acccgcgcaa tggcgcgggt
    24061 tttttgtttg actgcgtgct ggcttaatgc tggatgccgc tcactcgtct accgcgcaaa
    24121 gaagatgcgt ttagtagtag tagaccgata aagcgaacga tgtgagtcat taaatcagct
    24181 ccagatgaat gcgatatgct tttagagtta ctggatacaa aaacggatgt caaccctgac
    24241 gcaataaaaa cgtcccgcca gcatgagttc tgcatccgta agattagcta attgtgctgc
    24301 tgtggttaaa acaagcgata ttaatttctg cttaactacc gatgcttttc atcggttgac
    24361 atatttcaga ataaattttt gcatctaatc cacgaggaaa aaggggacaa aatgcacgcg
    24421 ttgcaaaacc tatcctgatg atttgtattg aattatatgt tttgcggatt ttttgatatt
    24481 gatttggtga atattattga tcaattaatg ttaagaatta atgcattaaa tatataaata
    24541 aattattaaa aagcacattt aatccatttt gtggatgatt gagtattcgc ggtagttatg
    24601 attagattgt ttacgcaaca agaacattat ggattattat gctgtggtaa atgacgcttt
    24661 ccacggcaat ggattctgtt tttataagaa cccgtatctt tatgttttcc taattttact
    24721 cattttcctt tttcttattt tatatgcatg ataaatcata ttctcgagga ttatttctct
    24781 gcattccaat aagggaaagg gagttaagtg tgacagtgga gttaagtatg ccagaggtac
    24841 aaacagatca tccagagacg gcggagttca gcaagccaca gctacgcatg gtcgatctca
    24901 acttattaac cgttttcgat gccgtgatgc aggagcaaaa cattacccgt gctgctcatg
    24961 ttctgggaat gtcgcaacct gcggtcagta acgctgttgc acgcctgaag gtgatgttta
    25021 atgacgagct ttttgttcgt tatggccgtg gtattcaacc gactgctcgc gcatttcaac
    25081 tttttggttc agttcgtcag gcattgcaac tagtacaaaa tgaattgcct ggttcaggtt
    25141 ttgaacccgc gagcagtgaa cgtgtatttc atctttgtgt ttgcagcccg ttagacagta
    25201 ttctgacctc gcagatttat aatcacattg agcagattgc gccaaatata catgttatgt
    25261 tcaagtcttc attaaatcag aacactgaac atcagctgcg ttatcaggaa acggagtttg
    25321 tgattagtta tgaagacttc catcgtcctg aatttaccag cgtgccatta tttaaagatg
    25381 aaatggtgct ggtagccagc aaaaatcacc caacaattaa gggcccgtta ctgaaacatg
    25441 atgtttataa cgaacaacat gcggcggttt cgctcgatcg tttcgcgtca tttagtcaac
    25501 cttggtatga cacggtagat aagcaagcca gtatcgcgta tcagggcatg gcaatgatga
    25561 gcgtacttag cgtggtgtcg caaacgcatt tggtcgctat tgcgccgcgt tggctggctg
    25621 aagagttcgc tgaatcctta gaattacagg tattaccgct gccgttaaaa ctaaatagca
    25681 gaacctgtta tctctcctgg catgaagctg ccgggcgtga taaaggccat cagtggatgg
    25741 aagagcaatt agtctcaatt tgcaaacgct aactgaatgc agaataggtc agacatgaat
    25801 gtctggttta ttctgcattt tttattgagt gtagaatttt attctgaatg tgtgggctct
    25861 ctattttagg attaattaaa aaaatag
//
LOCUS       DBFIFM010000048        25603 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.048, whole genome shotgun sequence.
ACCESSION   DBFIFM010000048 DBFIFM010000000
VERSION     DBFIFM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.048"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..476
                     /gene="bfr"
                     /locus_tag="N7C74_001730"
     CDS             <1..476
                     /gene="bfr"
                     /locus_tag="N7C74_001730"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HGU2431611.1"
                     /translation="KGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVEY
                     HESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLRE
                     AIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVND"
     gene            complement(473..940)
                     /locus_tag="N7C74_001731"
     CDS             complement(473..940)
                     /locus_tag="N7C74_001731"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HGU2431612.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1319..1630
                     /gene="rpsJ"
                     /locus_tag="N7C74_001732"
     CDS             1319..1630
                     /gene="rpsJ"
                     /locus_tag="N7C74_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HGU2431613.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1663..2292
                     /gene="rplC"
                     /locus_tag="N7C74_001733"
     CDS             1663..2292
                     /gene="rplC"
                     /locus_tag="N7C74_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HGU2431614.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2303..2908
                     /gene="rplD"
                     /locus_tag="N7C74_001734"
     CDS             2303..2908
                     /gene="rplD"
                     /locus_tag="N7C74_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HGU2431615.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2905..3207
                     /gene="rplW"
                     /locus_tag="N7C74_001735"
     CDS             2905..3207
                     /gene="rplW"
                     /locus_tag="N7C74_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HGU2431616.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3225..4046
                     /gene="rplB"
                     /locus_tag="N7C74_001736"
     CDS             3225..4046
                     /gene="rplB"
                     /locus_tag="N7C74_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HGU2431617.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4063..4341
                     /gene="rpsS"
                     /locus_tag="N7C74_001737"
     CDS             4063..4341
                     /gene="rpsS"
                     /locus_tag="N7C74_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HGU2431618.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4356..4688
                     /gene="rplV"
                     /locus_tag="N7C74_001738"
     CDS             4356..4688
                     /gene="rplV"
                     /locus_tag="N7C74_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HGU2431619.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4706..5407
                     /gene="rpsC"
                     /locus_tag="N7C74_001739"
     CDS             4706..5407
                     /gene="rpsC"
                     /locus_tag="N7C74_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HGU2431620.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5420..5830
                     /gene="rplP"
                     /locus_tag="N7C74_001740"
     CDS             5420..5830
                     /gene="rplP"
                     /locus_tag="N7C74_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HGU2431621.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5830..6021
                     /gene="rpmC"
                     /locus_tag="N7C74_001741"
     CDS             5830..6021
                     /gene="rpmC"
                     /locus_tag="N7C74_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HGU2431622.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6021..6275
                     /gene="rpsQ"
                     /locus_tag="N7C74_001742"
     CDS             6021..6275
                     /gene="rpsQ"
                     /locus_tag="N7C74_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HGU2431623.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6440..6811
                     /gene="rplN"
                     /locus_tag="N7C74_001743"
     CDS             6440..6811
                     /gene="rplN"
                     /locus_tag="N7C74_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HGU2431624.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6822..7136
                     /gene="rplX"
                     /locus_tag="N7C74_001744"
     CDS             6822..7136
                     /gene="rplX"
                     /locus_tag="N7C74_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HGU2431625.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7151..7690
                     /gene="rplE"
                     /locus_tag="N7C74_001745"
     CDS             7151..7690
                     /gene="rplE"
                     /locus_tag="N7C74_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HGU2431626.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7705..8010
                     /gene="rpsN"
                     /locus_tag="N7C74_001746"
     CDS             7705..8010
                     /gene="rpsN"
                     /locus_tag="N7C74_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HGU2431627.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8044..8436
                     /gene="rpsH"
                     /locus_tag="N7C74_001747"
     CDS             8044..8436
                     /gene="rpsH"
                     /locus_tag="N7C74_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HGU2431628.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8449..8982
                     /gene="rplF"
                     /locus_tag="N7C74_001748"
     CDS             8449..8982
                     /gene="rplF"
                     /locus_tag="N7C74_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HGU2431629.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8992..9345
                     /gene="rplR"
                     /locus_tag="N7C74_001749"
     CDS             8992..9345
                     /gene="rplR"
                     /locus_tag="N7C74_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HGU2431630.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9360..9863
                     /gene="rpsE"
                     /locus_tag="N7C74_001750"
     CDS             9360..9863
                     /gene="rpsE"
                     /locus_tag="N7C74_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HGU2431631.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9867..10046
                     /gene="rpmD"
                     /locus_tag="N7C74_001751"
     CDS             9867..10046
                     /gene="rpmD"
                     /locus_tag="N7C74_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HGU2431632.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10050..10484
                     /gene="rplO"
                     /locus_tag="N7C74_001752"
     CDS             10050..10484
                     /gene="rplO"
                     /locus_tag="N7C74_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HGU2431633.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10492..11823
                     /gene="secY"
                     /locus_tag="N7C74_001753"
     CDS             10492..11823
                     /gene="secY"
                     /locus_tag="N7C74_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HGU2431634.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11855..11971
                     /gene="rpmJ"
                     /locus_tag="N7C74_001754"
     CDS             11855..11971
                     /gene="rpmJ"
                     /locus_tag="N7C74_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HGU2431635.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12118..12474
                     /gene="rpsM"
                     /locus_tag="N7C74_001755"
     CDS             12118..12474
                     /gene="rpsM"
                     /locus_tag="N7C74_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HGU2431636.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12491..12880
                     /gene="rpsK"
                     /locus_tag="N7C74_001756"
     CDS             12491..12880
                     /gene="rpsK"
                     /locus_tag="N7C74_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HGU2431637.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12914..13534
                     /gene="rpsD"
                     /locus_tag="N7C74_001757"
     CDS             12914..13534
                     /gene="rpsD"
                     /locus_tag="N7C74_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HGU2431638.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13560..14549
                     /gene="rpoA"
                     /locus_tag="N7C74_001758"
     CDS             13560..14549
                     /gene="rpoA"
                     /locus_tag="N7C74_001758"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HGU2431639.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14590..14973
                     /gene="rplQ"
                     /locus_tag="N7C74_001759"
     CDS             14590..14973
                     /gene="rplQ"
                     /locus_tag="N7C74_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HGU2431640.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15080..15448
                     /locus_tag="N7C74_001760"
     CDS             15080..15448
                     /locus_tag="N7C74_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HGU2431641.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            15459..15884
                     /gene="zntR"
                     /locus_tag="N7C74_001761"
     CDS             15459..15884
                     /gene="zntR"
                     /locus_tag="N7C74_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HGU2431642.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15940..16158
                     /gene="arfA"
                     /locus_tag="N7C74_001762"
     CDS             15940..16158
                     /gene="arfA"
                     /locus_tag="N7C74_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HGU2431643.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16155..16565)
                     /gene="mscL"
                     /locus_tag="N7C74_001763"
     CDS             complement(16155..16565)
                     /gene="mscL"
                     /locus_tag="N7C74_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HGU2431644.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(16695..18071)
                     /gene="trkA"
                     /locus_tag="N7C74_001764"
     CDS             complement(16695..18071)
                     /gene="trkA"
                     /locus_tag="N7C74_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HGU2431645.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18093..19382)
                     /gene="rsmB"
                     /locus_tag="N7C74_001765"
     CDS             complement(18093..19382)
                     /gene="rsmB"
                     /locus_tag="N7C74_001765"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HGU2431646.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKSMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDTWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19428..20375)
                     /gene="fmt"
                     /locus_tag="N7C74_001766"
     CDS             complement(19428..20375)
                     /gene="fmt"
                     /locus_tag="N7C74_001766"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HGU2431647.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWVEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20390..20899)
                     /gene="def"
                     /locus_tag="N7C74_001767"
     CDS             complement(20390..20899)
                     /gene="def"
                     /locus_tag="N7C74_001767"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGU2431648.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21029..22153
                     /gene="dprA"
                     /locus_tag="N7C74_001768"
     CDS             21029..22153
                     /gene="dprA"
                     /locus_tag="N7C74_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HGU2431649.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLARQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLAKHGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGRP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22125..22598
                     /gene="smg"
                     /locus_tag="N7C74_001769"
     CDS             22125..22598
                     /gene="smg"
                     /locus_tag="N7C74_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HGU2431650.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22627..23169
                     /locus_tag="N7C74_001770"
     CDS             22627..23169
                     /locus_tag="N7C74_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HGU2431651.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCINYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23174..23746
                     /gene="tsaC"
                     /locus_tag="N7C74_001771"
     CDS             23174..23746
                     /gene="tsaC"
                     /locus_tag="N7C74_001771"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HGU2431652.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            23751..24569
                     /gene="aroE"
                     /locus_tag="N7C74_001772"
     CDS             23751..24569
                     /gene="aroE"
                     /locus_tag="N7C74_001772"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HGU2431653.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFV
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKPTADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            24566..24823
                     /locus_tag="N7C74_001773"
     CDS             24566..24823
                     /locus_tag="N7C74_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HGU2431654.1"
                     /translation="MNQAIQFPDREEWVENKKCVCFPALVNGMQLTCAISGDSLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24799..25353)
                     /locus_tag="N7C74_001774"
     CDS             complement(24799..25353)
                     /locus_tag="N7C74_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HGU2431655.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6561 a         6084 c         6657 g         6301 t
ORIGIN      
        1 tgaaaggtga tgttaaaatc ataaattatc tcaataaact attgggaaat gagcttgtcg
       61 caatcaatca gtattttctc catgcccgga tgtttaaaaa ctggggactg atgcgtctta
      121 atgacgtcga ataccatgaa tccatcgatg agatgaaaca cgctgataaa tatatcgagc
      181 gtattttgtt tttagaagga atccccaact tacaggattt aggtaagcta gggatcggtg
      241 aagatgtcga agagatgctg caatccgacc tgcgattaga gctggaaggt gcgaaggatc
      301 tgcgtgaggc tatcgcgtat gccgacagcg ttcatgacta tgttagccgc gatatgatga
      361 tcgagatcct tgccgatgaa gaaggtcata tcgactggct cgaaactgaa ctggatctca
      421 tcggtaaaat cggcctacaa aattacctgc aatcacaaat taaagttaat gattaacggc
      481 ctatcagcaa actatcccag cctataacga aacccgcagc cgccagaaat ggcccaaaag
      541 gcagcgggtt ttttaatgat tcttttcctc tcatgagcag accaataact acggctccgc
      601 aagcgaatga ggcagcaagg aaaaccagtc gtggtaagaa ggcccatgaa tgccaggcgc
      661 caagggcggc gagaaacttc acatcaccat agcctaatcc ttctttatgg cgcagtatgc
      721 gataccccca gtaaatgaca gcgaatgtgc cgtaaccaat aattgccccc cataacgcat
      781 cggctaaacc atcaggatga caaacttgat agaacaaaag acctgaccag agtaacgggc
      841 acgtaaatct gtcaggtaac aagccatgct ttgcatccca gaaaaaaagt agggctgata
      901 aacaagcgta caaaattaga aagggaaggg tagtgtccat aaaacatcct ctgtaattgg
      961 agtatcacca tcgaataact ccggatgctc agcaacagag agagcgctga tttgttaacc
     1021 tgattccggt gaaaaacgct atcttcacaa ccttacaaat ttcgtggaat gcagctcgca
     1081 atctgcgcgt tgcgattaat ttctgcccga tgcttgcgtc aggctcagat ttgcgtataa
     1141 tgcgcgggct tgtcgtagtt gacagcaggt tcaatctgaa cctcagttag ccgaatttgg
     1201 ctacctaaca atgctcccaa tcggggagct acgtaagaac ggttacactc tcccatcaat
     1261 cgtaatgggt ctgaggagta atcattttcg tttataaaat aattggagct ctggtctcat
     1321 gcagaaccaa agaatccgta tccgcctgaa agcgtttgat catcgtctga tcgatcaagc
     1381 aaccgcggaa atcgtcgaga ctgccaagcg cactggtgcg caggtccgtg gtccgatccc
     1441 gctgccgaca cgcaaagagc gcttcactgt tctgatctcc ccgcacgtca acaaagacgc
     1501 gcgcgatcag tacgaaatcc gtactcactt gcgtctggtt gacatcgttg agccaaccga
     1561 gaaaaccgtt gatgctctga tgcgtctgga tctggctgcc ggtgtagacg tgcagatcag
     1621 cctgggttaa tcaggtcatt gagcgattga gaggttgaaa caatgattgg tttagtcggt
     1681 aaaaaagtgg gtatgacccg tatcttcaca gaagacggcg tttctatccc agtaaccgta
     1741 atcgaagttg aagcaaaccg cgttactcag gttaaagacc tggctaacga tggctaccgt
     1801 gctattcagg tgaccaccgg tgctaaaaaa gctaaccgtg tgaccaagcc tgaagctggc
     1861 cacttcgcta aagctggcgt agaagctggc cgtggtctgt gggaattccg cctggctgaa
     1921 ggcgaagagt tcactgtagg tcagagcatt agcgttgaac tgtttgctga cgttaaaaaa
     1981 gttgacgtaa ctggcacctc taaaggtaaa ggtttcgcag gtaccgttaa gcgctggaac
     2041 ttccgtaccc aagacgctac tcacggtaac tccttgtctc accgcgttcc gggttctatc
     2101 ggtcagaacc agactccggg caaagtgttc aaaggcaaga aaatggcagg tcagatgggt
     2161 aacgaacgtg taaccgttca gagccttgac gtagtacgcg ttgacgctga gcgcaacctg
     2221 ctgctggtta aaggtgctgt cccgggtgca accggtagcg acctgatcgt taaaccagct
     2281 gtgaaggcgt aaggagatag caatggaatt agtattgaaa gacgcgcaga gcgcgctgac
     2341 tgtttccgaa actaccttcg gtcgtgattt caacgaagcg ctggttcacc aggttgttgt
     2401 tgcttatgca gctggtgctc gtcagggtac tcgtgctcag aagactcgtg ctgaagtaac
     2461 tggttccggt aaaaaaccgt ggcgccagaa aggcaccggc cgtgcgcgtt ctggttctat
     2521 caagagcccg atctggcgtt ctggtggcgt gacctttgct gctcgtccgc aggaccacag
     2581 tcaaaaagtt aacaagaaga tgtaccgcgg cgcgctgaaa agcatcctgt ccgaactggt
     2641 acgtcaggat cgtctgatcg ttgtcgagaa gttctctgta gaagcgccga aaactaagct
     2701 gctggcacag aaactgaaag acatggctct ggaagatgtg ctgatcatca ccggtgagct
     2761 ggacgaaaac ctgttcctgg ctgcgcgcaa cctgcacaag gttgacgtac gcgatgcaac
     2821 tggtatcgac ccggttagcc tgatcgcctt cgacaaagtc gtaatgactg ctgatgctgt
     2881 taagcaagtt gaggagatgc tggcatgatt cgtgaagaac gtctgctgaa ggtgctgcgt
     2941 gcaccgcacg tttctgaaaa agcgtctact gcgatggaaa aatccaacac catcgtactc
     3001 aaagttgcta aagacgcgac caaagcagaa atcaaagctg ctgtgcagaa actgtttgaa
     3061 gtcgaagtcg aagtcgttaa caccctggta gttaaaggga aagttaaacg tcacggacag
     3121 cgtatcggtc gtcgtagcga ctggaaaaaa gcttacgtca ccctgaaaga aggccagaat
     3181 ctggacttcg ttggcggcgc tgagtaagtc ggaggagtaa tacaatggca gttgttaaat
     3241 gtaaaccgac atctccgggt cgtcgccacg tagttaaagt ggttaaccct gagctgcaca
     3301 agggcaaacc ttttgctccg ttgctggaaa aaaacagcaa atccggtggt cgtaacaaca
     3361 atggccgtat caccactcgt catatcggtg gtggccacaa gcaggcttac cgtattgttg
     3421 acttcaaacg caacaaagac ggtatcccgg cagttgttga acgtcttgag tacgatccga
     3481 accgttccgc gaacatcgcg ctggttctgt acaaagacgg tgaacgccgt tacatcctgg
     3541 cccctaaagg cctgaaagct ggcgaccaga ttcagtctgg cgttgatgct gcaatcaaac
     3601 caggcaacac cctgccgatg cgcaacatcc cggttggttc tactgttcat aacgtagaaa
     3661 tgaaaccagg taaaggcggt cagctggcac gttccgctgg tacttacgtt cagatcgttg
     3721 ctcgcgacgg tgcttatgtc accctgcgtc tgcgttctgg tgaaatgcgt aaagtcgaag
     3781 cagactgccg tgcaactctg ggcgaagttg gcaatgctga gcatatgctg cgcgttctgg
     3841 gtaaagcagg tgctgcacgc tggcgtggtg ttcgtccgac cgttcgcggt accgcgatga
     3901 acccggtaga ccacccacat ggtggtggtg aaggtcgtaa ctttggtaag cacccggtaa
     3961 ctccgtgggg cgttcagacc aaaggtaaga agacccgcag caacaagcgt actgataaat
     4021 tcatcgtacg tcgccgtagc aaataatttt agaggataag ccatgccacg ttctctcaag
     4081 aaaggtcctt ttattgacct gcacttgctg aagaaggtag agaaagcggt ggaaagcgga
     4141 gacaagaagc ccctgcgcac ttggtcccgt cgttcaacga tctttcctaa catgatcggt
     4201 ttgaccatcg ctgtccataa tggtcgtcag cacgttccgg tatttgtaac cgacgaaatg
     4261 gtcggtcaca aactgggtga attcgcaccg actcgtactt atcgcggcca cgctgctgat
     4321 aaaaaagcga agaagaaata aggtaggagg aagagatgga aactatcgct aaacatcgcc
     4381 atgctcgttc ttctgctcag aaggttcgcc ttgttgctga cctgattcgc ggtaagaaag
     4441 tgtcgcaggc tctggatatt ttgacctaca ccaacaagaa agcggctgta ctggtcaaga
     4501 aagttctgga atctgccatt gctaacgctg aacacaacga tggcgctgac attgacgatc
     4561 tgaaagttac gaaaattttc gtagacgaag gcccgagcat gaagcgcatt atgccgcgtg
     4621 caaaaggtcg tgcagatcgc atcctgaagc gcaccagcca catcactgtg gttgtgtccg
     4681 atcgctgaga ctctggagac tagcaatggg tcagaaagta catcctaatg gtattcgcct
     4741 gggtattgta aaaccatgga actctacctg gtttgcgaac accaaagaat tcgctgacaa
     4801 cctggacagc gattttaaag tacgtcagta cctgactaag gaactggcta aagcgtccgt
     4861 atctcgtatc gttatcgagc gtccggctaa gagcatccgt gtaaccattc acactgctcg
     4921 cccgggtatc gttatcggta aaaaaggtga agacgtagaa aaactgcgta aggtcgtagc
     4981 ggacatcgct ggcgttcctg cacagatcaa catcgccgaa gttcgtaagc ctgaactgga
     5041 cgcaaaactg gttgctgaca gcatcacttc tcagctggaa cgtcgcgtta tgttccgtcg
     5101 tgctatgaag cgtgctgtac agaacgcaat gcgtctgggc gctaaaggta ttaaagttga
     5161 agttagcggc cgtctgggcg gcgcggaaat cgcacgtacc gaatggtacc gcgaaggtcg
     5221 cgtaccgctg cacactctgc gtgctgacat cgactacaac acctctgaag cgcacaccac
     5281 ttacggtgta atcggcgtta aagtgtggat cttcaaaggc gagatcctgg gtggtatggc
     5341 tgctgttgaa caaccggaaa aaccggctgc tcagcctaaa aagcagcagc gtaaaggccg
     5401 taaataagga gcgtcgctga tgttacaacc aaagcgtaca aaattccgta aaatgcataa
     5461 aggccgtaac cgcggtctgg cgcagggtac ggatgttagc ttcggcagct tcggtctgaa
     5521 agctgttggc cgtggtcgtc tgactgcccg tcagatcgaa gcagcacgtc gtgctatgac
     5581 ccgtgcagtt aagcgtcaag gtaagatctg gatccgtgtg ttcccggaca aaccgatcac
     5641 tgaaaagccg ctggcagtgc gtatgggtaa aggtaaaggt aacgtggagt attgggttgc
     5701 cttgattcag ccgggtaaag tcctgtatga aatggacggt gttccggaag agctggcccg
     5761 tgaagcattc aagctggcag cagcgaaact gccgattaaa accacctttg taactaagac
     5821 ggtgatgtaa tgaaagcaaa agagctgcgt gagaagagcg ttgaagagct gaacaccgag
     5881 ctgctgaacc tgctgcgtga gcagttcaac ctgcgtatgc aggctgcaag tggccagctg
     5941 caacagtctc acctgttgaa gcaagtgcgt cgcgatgtcg cacgcgttaa gactttactg
     6001 aacgagaagg cgggtgcgta atgaccgata aaatccgtac tctgcaaggt cgcgttgtta
     6061 gcgacaaaat ggagaaatcc attgttgttg ctatcgaacg ttttgtgaaa cacccgatct
     6121 acggtaaatt catcaagcgt acgaccaaac tgcacgtaca tgacgagaac aacgaatgcg
     6181 gtatcggtga cgtggttgaa atccgcgaat gccgtccgct gtccaagact aaatcctgga
     6241 cgctggttcg cgttgtagag aaagcggttc tgtaatacag tacactctct cgatacgaat
     6301 aaacggctca gaaatgagcc gtttattttt tctacccata tccttgaagc ggtgttataa
     6361 tgccgcgccc tcgatatggg gatttttaac gacctgattt tcgggtctca gtagtagttg
     6421 acattagcgg agcactaaaa tgatccaaga acagactatg ctgaacgtcg ccgacaactc
     6481 cggtgcacgt cgcgtaatgt gtatcaaggt tctgggtggc tcgcaccgtc gctacgcagg
     6541 cgtaggcgac atcatcaaga tcaccatcaa agaagcaatt ccgcgtggta aggtcaaaaa
     6601 aggtgatgtg ctgaaggcgg tagtggtgcg caccaagaag ggtgttcgtc gcccggacgg
     6661 ttctgtcatt cgcttcgatg gtaatgcttg tgttcttctg aacaacaaca gcgagcagcc
     6721 tatcggtacg cgtatttttg ggccggtaac tcgtgagctt cgtagtgaga agttcatgaa
     6781 aattatctct ctggcaccag aagtactcta aggagcgaat catggcagcg aaaatccgtc
     6841 gtgatgacga agttatcgtg ttaaccggta aagataaagg taaacgcggt aaagttaaga
     6901 atgtcctgtc ttccggcaag gtcattgttg aaggtatcaa cctggttaag aaacatcaga
     6961 agccggttcc ggccctgaac caaccgggtg gcatcgttga aaaagaagcc gctattcagg
     7021 tttccaacgt agcaatcttc aatgcggcaa ccggcaaggc tgaccgtgta ggctttagat
     7081 tcgaagacgg caaaaaagtc cgtttcttca agtctaacag cgaaactatc aagtaatttg
     7141 gagtagtacg atggcgaaac tgcatgatta ctacaaagac gaagtagtta aaaaactcat
     7201 gactgagttt aactacaatt ctgtcatgca agtccctcgg gtcgagaaga tcaccctgaa
     7261 catgggtgtt ggtgaagcga tcgctgacaa aaaactgctg gataacgcag cagcagacct
     7321 ggcagcaatc tccggtcaaa aaccgctgat caccaaagca cgcaaatctg ttgcaggctt
     7381 caaaatccgt cagggctatc cgatcggctg taaagtaact ctgcgtggcg aacgcatgtg
     7441 ggagttcttt gagcgcctga tcactattgc tgtacctcgt atccgtgact tccgtggcct
     7501 gtccgctaag tctttcgacg gtcgtggtaa ctacagcatg ggtgtccgtg agcagatcat
     7561 cttcccagaa atcgactacg ataaagtcga ccgcgttcgt ggtttggata ttaccattac
     7621 cactactgcg aaatctgacg aagaaggccg cgctctgctg gctgcctttg acttcccgtt
     7681 ccgcaagtaa ggtagggtta ctaaatggct aagcaatcaa tgaaagcacg cgaagtaaaa
     7741 cgcgtagctt tagctgataa atacttcgcg aaacgcgctg aactgaaagc gatcatctct
     7801 gatgtgaacg cttccgacga agatcgttgg aacgctgttc tcaagctgca gactctgccg
     7861 cgtgattcca gcccgtctcg tcagcgtaac cgctgccgtc aaacaggtcg tccgcatggt
     7921 ttcctgcgga agttcgggtt gagccgtatt aaggtccgtg aagccgctat gcgcggtgaa
     7981 atcccgggtc tgaaaaaggc tagctggtaa ttgtcaccaa ttgaatcacg ggagtaaaga
     8041 cagatgagca tgcaagatcc gatcgcggat atgctgaccc gtatccgtaa cggtcaggcc
     8101 gcgaacaaag ctgcggtcac catgccttcc tccaagctga aagtggcaat cgccaacgtg
     8161 ctgaaggaag aaggttttat tgaagatttt aaagttgaag gcgacaccaa gcctgaactg
     8221 gaacttactc tgaagtattt ccagggcaaa gctgttgtag aaagcattca gcgtgtcagc
     8281 cgcccaggtc tgcgcatcta taaacgtaaa gatgagctgc cgaaagttat ggcgggtctg
     8341 ggtatcgcag ttgtttctac ctctaaaggt gttatgactg atcgtgcagc gcgccaggct
     8401 ggtcttggtg gcgaaattat ctgctacgta gcctaatcgg aggaaaaaat gtctcgtgtt
     8461 gctaaagcac cggtcgttgt tcctgccggc gttgacgtaa aaatcaacgg tcaggttatt
     8521 acgatcaaag gtaaaaacgg cgagctgact cgtactctca acgatgctgt tgaagttaaa
     8581 catgcagata ataccctgac cttcggtccg cgtgatggtt acgcagacgg ttgggcacag
     8641 gctggtaccg cgcgtgccct gctgaactca atggttatcg gtgttaccga aggcttcact
     8701 aagaagctgc agctggttgg tgtaggttac cgtgcagcgg ttaaaggcaa tgtgattaac
     8761 ctgtctctgg gtttctctca tcctgttgac catcagctgc ctgcgggtat cactgctgaa
     8821 tgtccgactc agactgaaat cgtgctgaaa ggcgctgata agcaggtgat cggccaggtt
     8881 gcagcggatc tgcgcgccta ccgtcgtcct gagccttata aaggcaaggg tgttcgttac
     8941 gccgacgaag tcgtgcgtac caaagaggct aagaagaagt aaggtaacac tatggataag
     9001 aaatctgctc gtatccgtcg tgcgacccgc gcacgccgca agctccagga gctgggcgca
     9061 actcgcctgg tggtacatcg taccccgcgt cacatttacg cacaggtaat tgcaccgaac
     9121 ggttctgaag ttctggtagc tgcttctact gtagaaaaag ctatcgctga acaactgaag
     9181 tacaccggta acaaagacgc ggctgcagct gtgggtaaag ctgtcgctga acgcgctctg
     9241 gaaaaaggca tcaaagatgt atcctttgac cgttccgggt tccaatatca tggtcgtgtc
     9301 caggcactgg cagatgctgc ccgtgaagct ggccttcagt tctaaggtag aggtgtaaga
     9361 tggctcacat cgaaaaacaa gctggcgaac tgcaggaaaa gctgatcgcg gtaaaccgcg
     9421 tatctaaaac cgttaaaggt ggtcgtattt tctccttcac agctctgact gtagttggcg
     9481 atggtaacgg tcgcgttggt tttggttacg gtaaagcgcg tgaagttcca gcagcgatcc
     9541 agaaagcgat ggaaaaagcc cgtcgcaata tgattaacgt cgcgctgaat aacggcactc
     9601 tgcaacaccc tgttaaaggt gttcacacgg gttctcgcgt attcatgcag ccggcttctg
     9661 aaggtaccgg tatcatcgcc ggtggtgcaa tgcgcgccgt tctggaagtc gctggggttc
     9721 ataacgttct ggctaaagca tatggttcca ccaacccgat caacgtggtt cgtgcaacta
     9781 ttgatggcct ggaaaatatg aattctccag aaatggtcgc tgccaagcgt ggtaaatccg
     9841 ttgaagaaat tctggggaaa taaaccatgg caaagactat taaaattact caaacccgca
     9901 gtgcaatcgg tcgtctgccg aaacacaagg caacgctgct tggcctgggt ctgcgtcgta
     9961 ttggtcacac cgtagagcgc gaggatactc ctgctattcg cggtatgatc aacgcggttt
    10021 ccttcatggt taaagttgag gagtaagaga tgcgtttaaa tactctgtct ccggccgaag
    10081 gctccaaaaa ggcgggtaaa cgcctgggtc gtggtatcgg ttctggcctc ggtaaaaccg
    10141 gtggtcgtgg tcacaaaggt cagaagtctc gttctggcgg tggcgtacgt cgcggtttcg
    10201 agggtggtca gatgcctctg taccgtcgtc tgccgaaatt cggcttcact tctcgtaaag
    10261 cagcgattac agccgaagtt cgtctgtctg acctggctaa agtagaaggc ggtgtagtag
    10321 acctgaacac gctgaaagcg gctaacatta tcggtatcca gatcgagttc gcgaaagtga
    10381 tcctggctgg cgaagtaacg actccggtaa ctgttcgtgg cctgcgtgtt actaaaggcg
    10441 ctcgtgctgc tatcgaagct gctggcggta aaatcgagga ataagtagca gatggctaaa
    10501 caaccgggat tagattttca aagtgccaaa ggtggcttag gcgagctgaa acgcagactg
    10561 ctgtttgtta tcggtgcgct gattgtgttc cgtattggct cttttattcc gatccctggt
    10621 attgatgccg ctgtacttgc caaactgctt gagcaacagc gaggcaccat cattgagatg
    10681 tttaacatgt tctctggtgg tgctctcagc cgtgcttcta tctttgctct ggggatcatg
    10741 ccgtatattt cggcgtcgat cattatccag ctgctgacgg tggttcaccc aacgttggca
    10801 gaaattaaga aagaagggga gtctggtcgt cgtaagatca gccagtacac ccgttacggt
    10861 actctggtgc tggcaatatt ccagtcgatc ggtattgcta ccggtctgcc gaatatgcct
    10921 ggtatgcaag gcctggtgat taacccgggc tttgcattct acttcaccgc tgttgtaagt
    10981 ctggtcacag gaacgatgtt cctgatgtgg ttgggcgaac agatcactga acgaggtatc
    11041 ggcaacggta tttcaatcat tatcttcgcc ggtattgtcg cgggactccc gccagccatt
    11101 gcccatacta tcgagcaagc gcgtcaaggc gacctgcact tcctcgtgtt gctgttggtt
    11161 gcagtattag tatttgcagt gacgttcttt gttgtatttg ttgagcgtgg tcaacgccgc
    11221 attgtggtaa actacgcgaa acgtcagcaa ggtcgtcgtg tctatgctgc acagagcaca
    11281 catttaccgc tgaaagtgaa tatggcgggg gtaatcccgg caatcttcgc ttccagtatt
    11341 attctgttcc cggcgaccat cgcgtcatgg ttcgggggcg gtactggttg gaactggctg
    11401 acaacaattt cgctgtattt gcagcctggg caaccgcttt atgtgttact ctatgcgtct
    11461 gcaatcatct tcttctgttt cttctacacg gcgttggttt tcaacccgcg tgaaacagca
    11521 gataacctga agaagtccgg tgcatttgta ccaggaattc gtccgggaga gcaaacggcg
    11581 aagtatatcg ataaagtaat gacccgcctg accctggttg gtgcgctgta tattaccttt
    11641 atctgcctga tcccggagtt catgcgtgat gcaatgaaag taccgttcta cttcggtggg
    11701 acctcactgc ttatcgttgt tgtcgtgatt atggacttta tggctcaagt gcaaactctg
    11761 atgatgtcca gtcagtatga gtctgcattg aagaaggcga acctgaaagg ctacggccga
    11821 taattggtcg cccgagaagt tacggagagt aaaaatgaaa gttcgtgctt ccgtcaagaa
    11881 attatgccgt aactgcaaaa tcgttaagcg tgatggtgtc atccgtgtga tttgcagtgc
    11941 cgagccgaag cataaacagc gccaaggctg attttttcgc atatttttct tgcaaagttg
    12001 ggttgagctg gctagattag ccagccaatc ttttgtatgt ctgtgcgttt ccatttgagt
    12061 atcctgaaaa cgggcttttc agcatggaac gtacatatta aatagtagga gtgcatagtg
    12121 gcccgtatag caggcattaa cattcctgat cataagcatg ccgtaatcgc attaacttcg
    12181 atttatggcg tcggcaagac ccgttctaaa gccatcctgg ctgcagcggg tatcgctgaa
    12241 gatgttaaga tcagtgagct gtctgaagga caaatcgaca cgctgcgtga cgaagttgcc
    12301 aaatttgtcg ttgaaggtga tctgcgccgt gaaatcagca tgagcatcaa gcgcctgatg
    12361 gatcttggtt gctatcgcgg tttgcgtcat cgtcgtggtc tcccggttcg cggtcagcgt
    12421 accaagacca acgcacgtac ccgtaagggt ccgcgcaaac cgatcaagaa ataatcgggg
    12481 tgattgaata atggcaaagg caccaattcg tgcacgtaaa cgtgtaagaa aacaagtctc
    12541 tgacggcgtg gctcatatcc atgcttcttt caacaacacc atcgtgacta tcactgatcg
    12601 tcagggtaac gcgttgggtt gggcaacagc cggtggttcc ggtttccgtg gttctcgcaa
    12661 atccactccg tttgcagctc aggttgcagc agagcgttgc gctgacgccg tgaaagaata
    12721 cggcatcaag aatctggaag ttatggttaa aggtccgggt ccaggccgcg aatctactat
    12781 tcgtgctctg aacgccgcag gtttccgcat cactaacatt actgatgtga ctccgatccc
    12841 tcataacggt tgtcgtccgc cgaaaaaacg ccgcgtataa cgcctcgttt tccaggtttg
    12901 ttggagaaag aaaatggcaa gatatttggg tcctaagctc aagctgagcc gtcgtgaggg
    12961 caccgactta ttccttaagt ctggcgttcg cgcgatcgat accaagtgta aaattgaaca
    13021 agctcctggc cagcacggtg cgcgtaaacc gcgtctgtct gactatggtg tgcagttgcg
    13081 tgaaaagcaa aaagttcgcc gtatctatgg tgtgctggag cgtcagttcc gtaactacta
    13141 caaagaagca gcacgtctga aaggcaacac tggtgaaaac ctgttggctc tgctggaagg
    13201 tcgtctggac aacgttgtat accgtatggg cttcggtgcc actcgtgcag aagcacgtca
    13261 gctggttagc cataaagcaa ttatggtaaa cggtcgtgtt gttaacatcg cttcttatca
    13321 ggttagtccg aatgacgttg taagcattcg tgagaaagcg aagaagcagt ctcgcgtgaa
    13381 agccgctctg gagctggctg agcagcgtga aaagccaacc tggctggaag ttgatgctgg
    13441 caagatggaa ggtacgttta agcgtaagcc ggagcgttct gatctgtctg cggacattaa
    13501 cgaacacctg atcgtcgagc tttactccaa gtaaagctta gtaccaaaga gaggacacaa
    13561 tgcagggttc tgtgacagag tttctaaaac cgcgcctggt tgatatcgag caagtgagtt
    13621 cgacgcacgc caaggtgacc cttgagcctt tagagcgtgg ctttggccat actctgggta
    13681 acgcactgcg ccgtattctg ctctcatcga tgccgggttg cgcggtgacc gaggttgaga
    13741 ttgatggtgt actacatgag tacagcacca aagaaggcgt tcaggaagat atcctggaaa
    13801 tcctgctcaa cctgaaaggg ctggcggtga gagttcaggg caaagatgaa gttattctta
    13861 ccttgaataa atctggcatt ggccctgtga ctgcagccga tatcacccac gacggtgatg
    13921 tcgaaatcgt caagccgcag cacgtgatct gccacctgac cgatgagaac gcgtctatta
    13981 gcatgcgtat caaagttcag cgcggtcgtg gttatgtgcc ggcttctacc cgaattcatt
    14041 cggaagaaga tgagcgccca atcggccgtc tgctggtcga cgcatgctac agccctgtgg
    14101 agcgtattgc ctacaatgtt gaagcagcgc gtgtagaaca gcgtaccgac ctggacaagc
    14161 tggtcatcga aatggaaacc aacggcacaa tcgatcctga agaggcgatt cgtcgtgcgg
    14221 caaccattct ggctgaacaa ctggaagctt tcgttgactt acgtgatgta cgtcagcctg
    14281 aagtgaaaga agagaaacca gagttcgatc cgatcctgct gcgccctgtt gacgatctgg
    14341 aattgactgt ccgctctgct aactgcctta aagcagaagc tatccactat atcggtgatc
    14401 tggtacagcg taccgaggtt gagctcctta aaacgcctaa ccttggtaaa aaatctctta
    14461 ctgagattaa agacgtgctg gcttcccgtg gactgtctct gggcatgcgc ctggaaaact
    14521 ggccaccggc aagcatcgct gacgagtaac cggatcacag gttaaggttt tactgagaag
    14581 gataaggtca tgcgccatcg taagagtggt cgtcaactga accgcaacag cagccatcgc
    14641 caggctatgt tccgcaatat ggcaggttca ctggttcgtc atgaaatcat caagacgact
    14701 ctgcctaaag cgaaagagct gcgccgcgta gttgagccgc tgattactct tgccaagact
    14761 gatagcgttg ctaatcgtcg tctggcattc gcccgtactc gtgataacga gatcgtggca
    14821 aaactgttta acgaactggg cccgcgtttc gcgagccgtg ccggtggtta cactcgtatt
    14881 ctgaagtgtg gcttccgtgc aggcgacaac gcgccgatgg cttacatcga gctggttgat
    14941 cgttcagaga aagcagaagc tgctgcagag taatctgaag caacgtaaaa aaacccgccc
    15001 cggcgggttt ttttataccc gtagcatccc cacttatcta caatagctgt actctttttg
    15061 ttcatcccct ggagtattta tgtggttact tgaccagtgg gcagagcgcc atatagcaga
    15121 agcgcaagcg aaaggtgagt ttgataacct ggcaggtagc ggcgaaccat tgatactgga
    15181 tgatgattct catgtgccac cggaattacg tgcggggtat cgcttgctga agaatgccgg
    15241 ttgcttaccg ccagaacttg agcaacggag ggaagcaatc cagcttctgg atatcctcaa
    15301 aggtatccgt cacgatgatc cgcaatatca agaggttagc cgtcgattgt cattactgga
    15361 attgaagctg cgacaagctg gattgagtac cgatttttta cgcggcgatt atgctgacaa
    15421 gttgttgaat aaaatcaacg ataactagtg gagtatgtat gtatcgcatt ggtgagctgg
    15481 caaaaatggc ggaagtaaca cccgacacga ttcgttatta cgaaaagcag cagatgatgg
    15541 agcatgaagt gcgtaccgaa ggtgggtttc gcctgtatac cgaaagcgat ctccagcgat
    15601 tgaaatttat ccgccatgcc agacaactag gtttcagtct ggagtcgatc cgcgagttgc
    15661 tgtcgatccg tatcgatcct gaacaccata cctgtcagga gtcaaaaggc attgtgcagg
    15721 aaagattgca ggaagtcgaa gcacggatag ccgagttgca gagtatgcag cgttccttgc
    15781 aacgccttaa cgatgcctgt tgtgggaccg cccatagcag tgtttattgc tcgattcttg
    15841 aagctcttga acaaggggcg agtggcgtta agagtggctg ttgatttttt gcactggcag
    15901 gattacactc acgccgttaa ataaccaact ggagttttta tgagccgata tcagcatact
    15961 aaagggcaga taaaggataa tgcgatagaa gcattactac atgatccctt attccgacag
    16021 cgcgtagaga aaaataagaa ggggaaaggc agttacatgc gaaaaggaaa acatggcaat
    16081 cgggggaact gggaggccag tggcaagaaa gtaaatcact tttttaccac tggtcttctg
    16141 ctttcagggg cttgttaaga gcggttattc tgctctttca gcaaatcacg aatttctgtc
    16201 agtaatactt cttctttagt tggtgctggt gcggctgctg gttcttcttt tttccgattc
    16261 agtttgttga ttagcttaat cgccataaag atggcaaagg ccacaatcag aaaatcaaag
    16321 acgttttgaa tgaagacacc gtaatgcatc acaacagcag ggatatcccc ctgcgcatcg
    16381 cgtagcgtga cagcaaactg tttaaagtcg atcccgccaa ttaataagcc cagtggtggc
    16441 atgatgatat cggcaaccag tgaagataca atcttcccga atgccgcacc gataatgaca
    16501 cccaccgcca aatccaccac gttcccgcgc atcgcaaatt cgcgaaattc tttaataatg
    16561 ctcatgttat tctccctatg aagccgacaa ccataagtct aacaaatgtt aagccatttt
    16621 cctgccagga atgaatttaa ttaatccttg ataatcaaag ggaaataata aggcgtcatt
    16681 agacgcctta ttaattacaa gaagaaaggg cttggctgga agagtctttc gacgtcggta
    16741 ataaattttt tatctgtgag gaacataatt acgtgatcgc cttgctcaat gcgcagattg
    16801 tcattggcaa tcatcacgtc gtttccacgt accaccgctc caataatcgt tcctggcggt
    16861 agcttgattt cgtcaatgac tctgccgaca acgcgtgagg tgctttcatc accgtgagca
    16921 acggcttcaa tagcttctgc tacgccgcgg cgcaatgagg aaacaccaac aatatctgct
    16981 tttcgcacat ggctaagcaa cgcagaaata gttgcttgtt gtggtgaaat cgcaatatcg
    17041 atcacgctcc cctgaaccag atccacataa gcgcgacgct ggatcaatac catcaccttt
    17101 ttcgcaccca tacgtttggc aagcatggcg gacatgatat tggcctcgtc atcgttggtg
    17161 acggcaataa acagatcaac ttgatcgata tgttcttcgg ccagcagttc ttgatccgac
    17221 gcatcaccaa aaaagacgat cgtattctgc aacttttccg ccagttcggc agcgcgctgc
    17281 tgattacgtt cgatgagttt aacgctgtaa tctttttcca gacgacgcgc cagccctgca
    17341 ccgatattac cgccaccaac cagcatgatc cgcttatacg gtttttccag tcgctgtaat
    17401 tcactcatca ccgcgcggat atgctgtgaa gcggcaataa agaacacttc atcaccagcc
    17461 tcaacaatgg tcgaaccttg cggacgaatg gggcgatcgt ggcggaaaat tgctgccaca
    17521 cgagtatcga tatgtggcat atgttcgcgc atggtcgaaa gtgcattacc aatcagcggg
    17581 ccgccataat aggctttaac cacagcgagg ctgactttac cctcagcgaa gttcaccacc
    17641 tgcaatgcgc cgggatactc aatcagtcgg taaatattat cgataaccaa ctgctctggt
    17701 gcgatcagat gatcaatcgg tacagcatct gaatgaaata gcttatcggc atcgcgtacg
    17761 tagtctggtg agcggatacg agcgatgcga ttaggggtgt tgaaaagtga gtaggctacc
    17821 tggcaggcaa ccatattggt ttcatctgaa ctggttacag caaccagcat atcggcgtcg
    17881 tcggcacctg cctcccgcaa tacgcgtgga tgagagccat gcccctgcac gactcgcagg
    17941 tcaaatttat cctgcaaggt tcgcagacgc tcaccgttgg tatcgacaac agtgatatcg
    18001 ttgttctcgc caaccaggtt ttccgccagt gtgccgccaa cctggccggc acccagaatg
    18061 ataattttca tcagtcgcga cccgttatct catcactttt tgattagctt agcgtaaaag
    18121 aagccgtcgc cctcttcggc accaggcaga ttttgtttac ccggttgctc tggtgttcct
    18181 gtttcgcaaa gttcggcatc agcggtacgt tgcaaaaagg ctttaatctg caggctattc
    18241 tcttccggta acaccgaaca ggtggcatag accagggttc caccggattt taaatgcggc
    18301 caaatggcgt cgagaatttc agactgcaat tgcgcgagtt ctgggatatc gcgatcgcga
    18361 cgtaaccatt taatatccgg atggcgacga atcacaccgg ttgctgaaca aggcgcatct
    18421 aataaaatgc gatcaaactg ttgctcgcca caccattggg aagggtaacg gccatcacct
    18481 tgtttcacgg ttgccttcat accaaggcgt tttaaattgt cgtaaacgcg agagaggcgc
    18541 tgttcgtcaa tatcaaccgc aacaacctgc gcttctggtg ccacttcaag gatatgcgtt
    18601 gttttaccgc cgggggcggc acaaagatcc agaatgtgtt caccgttttg tggcgcaagc
    18661 caggtcatgc aaccttgtgc tgatgcatcc tgaacggtaa cccatccgtc ttcaaaacca
    18721 ggtagcgcat gaacaggtgc aggtgtttcc agccgtacag catcagggta atccgcatgc
    18781 gggaaacctt tcattcctgc ttcatccagc aatgcaagcc aggtgtcgcg ggaatgatgc
    18841 gtacgattaa tacgcagcca cattggcgga cgctggttat tggcttcgac gatggattgc
    18901 cactgctctg gatacgcttt ttgcagacgc ttcagcaacc aggaaggatg cagataacgt
    18961 gcatcactgg tattaaactc ggctaataac tcttcttgct gacgctggaa ctggcgtaat
    19021 acaccgttaa tcaacccttt aagttgcgga cgcttaattg cgatagcgcc ttcaaccgtt
    19081 tcagccagcg cagcatgagg tggaatgcgg gtataaagca gttgatacaa accaaccata
    19141 atcaggtaat gcacggtccg ctgtttgccg gtcatcggac gggccattga cttattaatc
    19201 agccagtcta actgcgaaag cgtacgcagt acgccaaagc acaactcttg aagaagtgct
    19261 ttgtctttat cggaaacttt ttgctggagc ggtggcagaa tgttgcttaa tgattgccct
    19321 tgctcgacga cttgttcaac ggcctgggcc gccatgctac gtaaattacg ttgttttttc
    19381 ataagtataa aaatgcccgg caagaccggg cttagaagag tggactatca gaccagacgg
    19441 ttgcccggaa caaaccattc ccgacgagag ttcaggaggt cttgcgcgct catcgctttc
    19501 ttacccgcag gttgtaacga gagcaggttc aggatgccat caccagtcgc aacctgaatg
    19561 ccttgtttgt tggcttcaag gatcgttcct ggtgcagcgt tggttgccgt atcaatgacc
    19621 gatgctttcc agactttaac cggctgtcct tcaatttcca cccagctcat tggccatgga
    19681 ttgaaagcgc gaatgcagcg ttcaagctgt gctgccgaaa gtgaccagtc aatacgcgct
    19741 tcttctttac tcaacttctc ggcgtaagtg acaagagttt cgtcctgaac ttctggtttc
    19801 gccgtgccgt ctgccaattg tttcaacgtg gtgataagcc cttgtgggcc aagctctgcc
    19861 agcttgtcgt acagcgtacc actggtatct tctgcagtaa tcgggcagga gagcttatag
    19921 agcatgtcac cggtgtctaa accaacatcc atttgcataa tggtcacacc agtttctgca
    19981 tcacccgccc atagtgagcg ttggattggt gcagcaccgc gccagcgtgg cagcagtgaa
    20041 ccatgaacgt tgatacagcc aagacgcggc atctccagca ctgctttagg cagaattaag
    20101 ccataggcga cgacaaccat aacatcagcc tgtagatcgg cgaccagttg ctggttttct
    20161 tgtggacgca gggaaacagg ttgaaaaacg ggcagacctt tttcctcagc cagaacttta
    20221 accgggctgg gcatcagttt tttaccgcgt cctgccggtc ggtctggctg ggtgaacacg
    20281 ccaacgacgt tatgaccaga agacaacagc gcgtcgagat gacgcgctgc aaagtcaggt
    20341 gtacccgcaa aaataatacg tagtgattct gacacgttag ttcttatcct taagcccggg
    20401 ctttcagacg atccagtttt tcaactttct gacgaatacg ttgttgtttc agcggtgaca
    20461 gataatccat aaacagtttg ccgaccaggt gatccatctc atgctgaata cagatggcta
    20521 acagaccatc tgcctccagt tcaaatggtt taccgtcgcg gtccagggcg cgaattttaa
    20581 ctttctctgc gcgtggcact aaagcacgtt gttcagggat cgacaggcaa ccttcttcaa
    20641 tgcctgtttc gccgcttttt tctaaaagct ccggattgat taacaccagc cgttcgtcac
    20701 ggttttccga aacatcaata acaatgatac gttgatggat atcaacctgg gttgccgcca
    20761 ggccaatacc ttcttctgcg tacatcgtct cgaacatatc atcgacgata cgctgaattt
    20821 ctgcattcac ttcttctacc ggtttagcaa ctttgcgaag ccgctcgtcc ggaatatgta
    20881 acacttgcaa aactgacata aatctccaga gatgtgttca ggagttagaa agattatttc
    20941 ttctattcta gacaaatccc cctctgattg acagcatcac tgaccaatcg caaagattgc
    21001 taaggctgct tatggcaggg agataaggat ggtcgataca gaaatttggc tgcgtttaat
    21061 gagtatcagc agcttgtacg gcgatgatat ggtccgtata gctcactggc tggcaagaca
    21121 gtcgcatatt gatgcggttg tattgcagca aacagggctt acattgcggc aggcacaacg
    21181 ctttctttca tttccgcgga agagtatcga aagctcactt tgttggttgg agcaacccaa
    21241 ccatcattta atccctgcgg acagcgaatt ttatcctcct caacttctgg cgacgacaga
    21301 ttaccccggt gcactgtttg ttgaaggaga actgcacgcg ctgcattcat ttcagcttgc
    21361 cgtagtgggg agtcgggcgc attcatggta tggcgagcga tggggacgat tattttgcga
    21421 aactctggcg aagcatggag tgacaattac gagtggactg gcgcgtggaa tcgatggtgt
    21481 agcgcataaa gcagccttac aggtaaatgg cgtcagcatt gctgtattgg ggaatggact
    21541 taataccatt catccccgcc gtcatgcccg actggctgcc agtctgcttg aacagggggg
    21601 cgctctcgtc tcggaatttc ccctcgatgt tccacccctt gcttacaatt tcccacgaag
    21661 aaatcgcatt atcagtggtc taagtaaagg tgtactggtg gtggaagcgg ctttgcgtag
    21721 tggttcgctg gtgacagcac gttgtgcgct tgagcagggg cgagaagttt ttgccttgcc
    21781 aggtccaata gggaatccgg gaagcgaagg gcctcactgg ttaataaaac aaggtgcgat
    21841 tcttgtgacg gaaccggaag aaattctgga aaacttgcaa tttggattgc actggttgcc
    21901 agacgcccct gaaaattcat tttattcacc agatcaggaa gacgtggcat tgccatttcc
    21961 tgagctcctg gctaacgtag gagatgaggt aacacctgtt gacgtcgtcg ctgaacgtgc
    22021 cggccgacct gtgccagagg tagttactca actactcgaa ctggagttag caggatggat
    22081 cgcagctgta cccggcggct atgtccgatt gaggagggca tgccatgttc gacgtactaa
    22141 tgtatttgtt tgaaacctat attcacacag aagctgagtt gcgtgtggat caagacaaac
    22201 ttgaacagga tcttaccgac gcaggatttg atcgagaaga tatctacaat gccctgcttt
    22261 ggctggaaaa gcttgcggat tatcaggaag gactggcaga accgatgcaa ctggcctctg
    22321 atccactctc catgcgtatt tatacccctg aggagtgtga aagactggat gccagctgtc
    22381 gtggttttct gcttttcctt gagcaaattc aggtgcttaa ccttgaaacc cgtgaaatgg
    22441 tgatagagcg agtgctcgcg ctggataccg cagagttcga cctggaagac ctgaaatggg
    22501 taatcctgat ggtgttgttc aatattccgg gctgcgaaaa tgcgtaccag caaatggaag
    22561 aattactctt tgaagtgaat gaaggtatgc tgcattaatc atttctttaa ttcagcataa
    22621 gttgttatgg cgaaatcagc actgttcacg gtgcgtaata atgagtcctg cccaaagtgc
    22681 ggggctgaac tggttattcg atccgggaaa cacggtccgt ttcttggatg ctcacagtat
    22741 ccggcgtgtg actacgtccg tcctctgaaa tcttcagcgg atggacatat cgtcaaagtt
    22801 ctggaggggc aggtttgccc tgcatgtggc gcaaatctgg tattacgcca gggacgcttt
    22861 ggtatgttta ttggttgcat taactaccct gaatgcgaac ataccgaact tatcgataaa
    22921 ccggacgaaa cagcaattac atgtccccaa tgccggacgg gccatctggt ccagcgccgc
    22981 tcccgttatg gcaaaacatt tcactcctgt gatcgctacc cggagtgtca atttgccatt
    23041 aacttcaaac ccatagctgg agaatgccct gagtgtcatt atccgctact catcgaaaag
    23101 aaaaccgcgc agggtgtaaa acacttttgt gccagtaaac aatgtggaaa gccggtttcg
    23161 gcggaataat aacgtgaata ataacctgca aggagacgct atcgcagctg cgatagatgt
    23221 tctcaatgaa gaacgtgtca tcgcctatcc aacggaagcc gttttcggtg ttgggtgcga
    23281 tcctgatagc gaaacagcag tgatgcgact gttggagtta aaacagcgtc cggttgataa
    23341 ggggctgatt ttaatcgcag caaattacga gcagcttaaa ccctatattg atgacaccat
    23401 gttgactgac gtgcagcgtg aaaccatttt ttcccgctgg ccaggtcctg tcacctttgt
    23461 ctttcccgcg cctgcgacaa caccgcgctg gttgacgggc cgctttgatt cgcttgctgt
    23521 acgagtcacc gaccatccgt tggtggttgc tttgtgccag gcttatggta aaccgctggt
    23581 ttctaccagt gccaacttga gtggattgcc accttgtcga acagtagacg aagttcgcgc
    23641 acaatttggc gcggcgttcc cggttgtgcc tggtgaaacg ggggggcgtt taaatccttc
    23701 agaaatccgc gatgccctga cgggtgaact gtttcgacag gggtaacata atggaaacct
    23761 atgctgtttt tggtaatccg atagcccaca gcaaatcgcc attcattcat cagcaatttg
    23821 ctcagcaact gaatattgaa catccctatg ggcgcgtgtt ggcacccatc aatgatttcg
    23881 tcaacacact gaacgctttc tttagtgctg gtggtaaagg tgcgaatgtg acggtgcctt
    23941 ttaaagaaga ggcttttgcc agagcggatg agcttactga acgcgcagcg ttggctggtg
    24001 ctgttaacac cctcaagcgg ttagaagatg gacgcttgct gggtgacaat accgatggtg
    24061 taggcttgtt aagcgatctg gaacgtctgt cttttatccg tcctggttta cgtattctgc
    24121 tcatcggcgc aggtggagca tctcgcggcg tactactgcc acttctttcc ctggactgtg
    24181 cggtgacaat cactaatcgg acggtatccc gagcggaaga gttggctaaa ttgtttgcgc
    24241 acactggcag tattcaggcg ttgggtatgg acgaactgga aggtcatgag tttgatctca
    24301 ttattaatgc cacatccagt ggcatcagtg gtgatattcc ggcgatcccg tcatcgctca
    24361 ttcatccagg catttattgc tatgacatgt tctatcagaa aggaaaaact ccttttttgg
    24421 catggtgtga gcagcgaggc tcaaagccta ctgctgatgg tttaggaatg ctggtggcac
    24481 aggcggctca tgccttcctt ctttggcatg gcgttctgcc tgatgtagaa ccagttataa
    24541 agcaattgca ggaggaattg tccgcgtgaa tcaggccatc cagtttccgg acagagaaga
    24601 gtgggtcgag aataaaaaat gtgtatgttt tcccgctctc gtgaatggca tgcaactgac
    24661 atgtgcgatc tctggcgata gcctggcgta tcgctttaca ggagatacgc cagagcagtg
    24721 gttagcgagt tttcgtcagc atcgctggga cttggaagaa gaagcggaaa acttaattca
    24781 ggaacaaagt gaagatgatc aaggctgggt ctggttaccc tgatccagat attcgtcctt
    24841 ccatttcacg taattattcg cggaatagcg taacccagcc ttctcttcat cacttaacgg
    24901 gcggatctgt ttgacgggac taccgagata cagatatccg ctctccagcc gtttattttg
    24961 tgggaccaga ctacccgcac caatcatcac atcatcttct actattgcgc catcaagtaa
    25021 aattgagccc atcccaacca aaactcgatt gccaatggtg cagccgtgga gcatcacctt
    25081 gtgaccaaca gtgacatctt cgccaatggt taatgggttg ccatctgggt tgtacgagga
    25141 tttatgagtg acatgcaaca tactgccatc ctggatattg gtgcgtgctc cgatctgtac
    25201 ataatgtaca tctccacgaa tcacaacgag cggccagatc cccacatcat cagccagacg
    25261 aacgtcacca atcacgacac tgctatcgtc gatcattacg cgctgaccga tttgtggaaa
    25321 aagatcgcgg tatgggcgta aaacatcaga catacttacc tcagcaataa atgatttact
    25381 aatgactttg ggggcattat tggccttgtg caagtctttt agtatgcaaa aaagcaccgt
    25441 tttgtgtgcg aatgcagcaa aaagggtgaa aaaacaacaa acagaaaaaa agatcaaaaa
    25501 agtacttgtg caaaaaattg ggatccctat aatgcgcctc cgttgagacg acaacgtgaa
    25561 acacttcaca agatggtcgt aacaacgaag agaaaaaatc ctg
//
LOCUS       DBFIFM010000049        25276 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.049, whole genome shotgun sequence.
ACCESSION   DBFIFM010000049 DBFIFM010000000
VERSION     DBFIFM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.049"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            150..1289
                     /gene="wza"
                     /locus_tag="N7C74_001775"
     CDS             150..1289
                     /gene="wza"
                     /locus_tag="N7C74_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HGU2431656.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1295..1738
                     /gene="wzb"
                     /locus_tag="N7C74_001776"
     CDS             1295..1738
                     /gene="wzb"
                     /locus_tag="N7C74_001776"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HGU2431657.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            1741..3903
                     /gene="wzc"
                     /locus_tag="N7C74_001777"
     CDS             1741..3903
                     /gene="wzc"
                     /locus_tag="N7C74_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HGU2431658.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMEDQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQILKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            3996..4835
                     /gene="wcaA"
                     /locus_tag="N7C74_001778"
     CDS             3996..4835
                     /gene="wcaA"
                     /locus_tag="N7C74_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HGU2431659.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACTVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            4838..5326
                     /gene="wcaB"
                     /locus_tag="N7C74_001779"
     CDS             4838..5326
                     /gene="wcaB"
                     /locus_tag="N7C74_001779"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HGU2431660.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5323..6540
                     /gene="wcaC"
                     /locus_tag="N7C74_001780"
     CDS             5323..6540
                     /gene="wcaC"
                     /locus_tag="N7C74_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HGU2431661.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKGVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQALFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6515..7732
                     /gene="wcaD"
                     /locus_tag="N7C74_001781"
     CDS             6515..7732
                     /gene="wcaD"
                     /locus_tag="N7C74_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HGU2431662.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKITQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGICAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            7743..8489
                     /gene="wcaE"
                     /locus_tag="N7C74_001782"
     CDS             7743..8489
                     /gene="wcaE"
                     /locus_tag="N7C74_001782"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HGU2431663.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAAYF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWVELSWHLRQRTTSKTKALYNKS"
     gene            8505..9053
                     /gene="wcaF"
                     /locus_tag="N7C74_001783"
     CDS             8505..9053
                     /gene="wcaF"
                     /locus_tag="N7C74_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HGU2431664.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYVWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRKRVETE"
     gene            9082..10203
                     /gene="gmd"
                     /locus_tag="N7C74_001784"
     CDS             9082..10203
                     /gene="gmd"
                     /locus_tag="N7C74_001784"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HGU2431665.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10206..11171
                     /gene="fcl"
                     /locus_tag="N7C74_001785"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10206..11171
                     /gene="fcl"
                     /locus_tag="N7C74_001785"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HGU2431666.1"
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11174..11653
                     /gene="gmm"
                     /locus_tag="N7C74_001786"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11174..11653
                     /gene="gmm"
                     /locus_tag="N7C74_001786"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HGU2431667.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            11650..12873
                     /gene="wcaI"
                     /locus_tag="N7C74_001787"
     CDS             11650..12873
                     /gene="wcaI"
                     /locus_tag="N7C74_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HGU2431668.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            12876..14312
                     /gene="cpsB"
                     /locus_tag="N7C74_001788"
     CDS             12876..14312
                     /gene="cpsB"
                     /locus_tag="N7C74_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HGU2431669.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEDAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14505..15875
                     /gene="cpsG"
                     /locus_tag="N7C74_001789"
                     /gene_synonym="manB"
     CDS             14505..15875
                     /gene="cpsG"
                     /locus_tag="N7C74_001789"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HGU2431670.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDARLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPSSGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            15930..17324
                     /gene="wcaJ"
                     /locus_tag="N7C74_001790"
     CDS             15930..17324
                     /gene="wcaJ"
                     /locus_tag="N7C74_001790"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HGU2431671.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKLGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17326..18804
                     /gene="wzxC"
                     /locus_tag="N7C74_001791"
     CDS             17326..18804
                     /gene="wzxC"
                     /locus_tag="N7C74_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HGU2431672.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYVLGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            18984..20264
                     /gene="wcaK"
                     /locus_tag="N7C74_001792"
     CDS             18984..20264
                     /gene="wcaK"
                     /locus_tag="N7C74_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HGU2431673.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQVYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGVMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            20261..21481
                     /gene="wcaL"
                     /locus_tag="N7C74_001793"
     CDS             20261..21481
                     /gene="wcaL"
                     /locus_tag="N7C74_001793"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HGU2431674.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THVAWTKYNLAARTRWLQDEPTGKVAKLSHRASQTLRGIHRKNTWQALNLKRYGAESW
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGVIHGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21492..22886
                     /gene="wcaM"
                     /locus_tag="N7C74_001794"
     CDS             21492..22886
                     /gene="wcaM"
                     /locus_tag="N7C74_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HGU2431675.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARAFPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTI
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITQDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGNYLSIPQNF
                     RVNNIQLDNTHLAYKLRGLQISAGNAVSFVALTNIEMKRASLELHNKPQHLFLRNIKV
                     MQESSVGPALIMNFDMRKDVRGVFMAKEETLLSLANVHAVNEKGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23044..24039
                     /locus_tag="N7C74_001795"
     CDS             23044..24039
                     /locus_tag="N7C74_001795"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HGU2431676.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            24282..25175
                     /gene="galF"
                     /locus_tag="N7C74_001796"
     CDS             24282..25175
                     /gene="galF"
                     /locus_tag="N7C74_001796"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HGU2431677.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
BASE COUNT         6225 a         6284 c         6849 g         5918 t
ORIGIN      
        1 attctgtcaa tagcctgcgg atgaaatcaa tttttttagg actgatgcca gttaaatttt
       61 tattcaatta actgcaaggt aaatgtgcat ggcgacattg ctattaatag tgcacaggat
      121 aattactctg ccaaagtgat aaataaacaa tgatgaaatc caaaatgaaa ttgatgccat
      181 tattggtgtc agtaaccttg ataagcggtt gcacagtact tccgggcagc aatatgtcga
      241 cgatgggcaa agacgtcatc aaacagcagg acgctgattt cgatctcgac aaaatggtga
      301 atgtttatcc gctgaccccg cgcctgattg accaattacg cccacgcccg aatgtagcgc
      361 gccccaatat gacgctggaa agtgagatcg cgaattacca gtatcgcgtc gggccggggg
      421 acgttcttaa tgtcaccgtc tgggatcacc cggaactcac cacgccagcc ggtcagtacc
      481 gcagctccag cgacaccggc aactgggtac agcctgacgg cactatgttt tacccgtata
      541 tcggcaaggt tcacgtagtc gggaaaacgc tcgctgaaat ccgcagtgat attaccgggc
      601 gcttagcgac gtacatcgct gacccgcagg tggacgttaa tatcgccgcc ttccgctcac
      661 aaaaggccta tatctcaggt caggtgaata aatctggtca acaggcgatc accaacgtac
      721 cactgaccat tctcgacgcc atcaacgccg caggtggcct gaccgacacc gctgactggc
      781 gcaacgtggt gctaacacac aatggtcgtg aagagcgaat ttctttgcag gcgctgatgc
      841 aaaacggcga cctcaatcag aaccgactgc tttaccccgg cgatattctc tacgtgccgc
      901 gtaatgatga tctgaaagtg tttgtgatgg gtgaagtgaa gaaacagagc accctgaaaa
      961 tggactttag cggcatgacc ctgaccgaag ccctgggcaa tgctgaaggc atcgacatga
     1021 ccacctccaa cgccagcggc atctttgtca ttcgtccgct gaaaggcgag ggcgggcgta
     1081 acggcaaaat tgccaatatc taccagctgg atatgtccga tgccacgtcg ctggtgatgg
     1141 cgacagaatt ccgcctgcaa ccttatgacg tggtgtatgt caccaccgcc ccggtttccc
     1201 gctggaaccg tctgatcaat cagttgctgc caactattag cggtgtccgt tacatgacgg
     1261 atacagccag cgacattcat aactggtaat cgtcatgttt aacaacatct tagttgtctg
     1321 tgtcggcaat atttgccgtt ccccgacggc ggaacgctta ctgcaacgtt atcacccgga
     1381 gctgaaagtg gagtccgctg gactcggcgc gctggtcggt aagggcgctg atcctaccgc
     1441 tatcagcgtc gccgcagaac atcaactgtc tctggaaggc cactgtgcac gacaaatcag
     1501 ccgccgcctg tgtcgcaact atgacctgat tttgaccatg gaaaagcgcc atatcgaacg
     1561 cttatgcgag atggcaccgg agatgcgcgg caaagtgatg ctgtttggtc actgggataa
     1621 cgaatgtgaa atccccgatc cgtatcgcaa aagccgggaa acgtttgcag cggtgtacac
     1681 attacttgaa cggtctgccc gccagtgggc gcaggcattg aacgcagagc aggtataaga
     1741 atgacagaaa aagtaaaaca acatgccgct ccggtaacgg gcagtgatga aatcgatatt
     1801 ggtcgcctgg tcggcaccgt cattgaagcg cgctggtggg tgattggcat caccgctgta
     1861 ttcgcccttt gtgccgtggt ttacaccttc ttcgccacgc cgatttatag tgccgacgca
     1921 ctggtacaaa tcgagcaaag cagcggcaat tcgttagtgc aggatatcgg atcggcgtta
     1981 gccaacaaac cgcctgcatc ggacgccgag atccagttga ttcgttcgcg cctggtgctt
     2041 ggtaaaacgg tggatgatct cgacctcgat attgcggtga gcaaaaacac gttcccgatt
     2101 ttcggtgcgg gctgggatcg cctgatggga cgccagaacg agacggtgaa agtgactacc
     2161 tttaaccgtc cgaaagagat ggaggatcag gtgtttacgc ttaatgtgct ggacaacaaa
     2221 aactacaccc tgagcagcga tggcggcttt agcgcccgtg ggcaagcggg ccagatactg
     2281 aaaaaagaag gcgtcacgct gatggttgaa gccattcacg cccgcccggg cagtgagttt
     2341 accgtcacca aatactccac gctggggatg atcaatcaac tgcaaaacag cctgacggta
     2401 acggagaacg gcaaagacgc aggcgtactg agcctgactt ataccggtga agatcgcgaa
     2461 cagatccgcg acattcttaa cagcatcgcc cgtaactatc aggaacaaaa tattgagcgc
     2521 aaatcggcgg aagcgtcgaa aagcctcgct ttcctcgcgc aacagttacc ggaagtacgt
     2581 agccgccttg atgttgccga aaacaaactg aatgccttcc gtcaggataa agattctgtt
     2641 gatctgccgc tggaagcgaa agcggtgctc gattcgatgg tgaacatcga cgcccagttg
     2701 aacgaactga cctttaaaga ggcggaaatc tccaagctgt acaccaaagt tcaccccgcg
     2761 taccgcacgc tgctggagaa acgtcaggcg ctggaagacg aaaaagccaa acttaatggt
     2821 cgcgtaacgg cgatgccgaa aacccagcag gaaattgtcc gtctgacccg cgatgtcgag
     2881 tctggtcagc aggtctatat gcaactgctg aataaagagc aggagctgaa aatcaccgag
     2941 gccagcaccg tcggcgatgt gcgcattgtt gacccggcaa tcactcagcc tggtgtgcta
     3001 aaaccgaaga aagggctgat tatccttggg gcgattatcc ttggcctgat gctctctatc
     3061 gtgggggtgc tgctgcgctc gttgtttaat cgcggcatcg aaagcccgca ggtgctggaa
     3121 gaacacggta tcagcgtcta tgccagcatc ccgctgtcgg agtggcagaa agcgcgcgat
     3181 agcgtcaaaa ccatcaaagg gattaaacgc tataaacaga gccagctact ggcggtgggg
     3241 aatccaaccg atctggcgat tgaagccatc cgcagccttc gtaccagttt gcacttcgcg
     3301 atgatgcagg cgcagaacaa tgtgttgatg atgaccgggg ttagcccgtc aatcggtaaa
     3361 acctttgtct gcgccaacct ggcggcggtg atcagccaga ccaataaacg cgtgttgttg
     3421 atcgactgcg atatgcgcaa aggctacacc catgagctgt tgggcactaa taacgttaat
     3481 ggcctgtcgg aaattctgat tggtcagggc gatattacta cagctgctaa accgacctct
     3541 attgccaaat ttgacctgat cccgcgcggt caggtaccgc caaatccttc tgaactgttg
     3601 atgagcgaac gctttgccga actggtgaac tgggcgagta aaaactacga cctggtgttg
     3661 attgatacgc cgccgattct ggcagtgacc gatgcggcaa ttgttggtcg tcatgtcgga
     3721 accacgttaa tggtggcgcg ttatgcggtc aacacattga aagaagtgga aaccagtctg
     3781 agccgctttg agcaaaacgg tattccggtg aaaggggtga ttctgaactc catcttccgc
     3841 cgcgccagcg cgtatcagga ttatggctat tacgaatacg aatataagtc ggatgcgaaa
     3901 taacgaggcc tgcattcgca ccgccccgta ggccggataa ggcgctcacg ccgcatccgg
     3961 caagcaaacc agctcataag ccgggagtac aacctatgaa agacaatccg ctgatctcaa
     4021 tctatatgcc gacctggaac cgccaacaac tggcgattcg ggcgataaaa tcggtcttgc
     4081 gccaggacta cagcaactgg gagatgatca tcgtggatga ttgctccacc tcctgggaac
     4141 aactgcaaca gtacgtcacc gccctcaacg atccgcgtat tacctacatt cacaacgaca
     4201 ttaacagcgg ggcgtgcacg gtacgtaacc aggcgattat gctggcgcaa ggggaataca
     4261 ttaccgggat cgatgacgat gatgaatgga cgcccaaccg cctgagcgtc ttcctcgccc
     4321 ataaacagca actggtcact cacgcctttt tgtacgctaa cgattacgtt tgccagggcg
     4381 aagtttattc ccaaccggca agcttaccac tgtatcccaa atcgccgtac tcacgccgcc
     4441 tgttctataa acgcaatatc attggcaatc aggtctttac ctgggcatgg cgtttcaaag
     4501 agtgtttgtt cgataccgaa ctgaaagcgg cacaggatta cgacatcttc ctgcggatgg
     4561 tggtggagta tggcgaaccg tggaaagtgg aagaggcgac gcagatcctg catatcaatc
     4621 acggtgaaat gcagatcacc tcgtcaccga aaaaattctc cggatacttc cacttttacc
     4681 gcaagcacaa agacaaattc gaccgtgcca gtaaaaaata tcagctgttt accctctacc
     4741 agatccgcaa taaacgtatg acctggcgca ctttgctgac gctgctgtcg gtgcgtaacg
     4801 gcaaacgtct ggctgatggt attcgggggc gctaaatatg ctggaagatc tacgcgccaa
     4861 cagctggagt ttacgcccgt gctgcatggt tcttgcctat cgcgtcgctc atttttgttc
     4921 ggtgtggcgc aaaaagaacg ttctcaacaa tctttgggcg gccccgctgc tggtgctgta
     4981 tcgcattatc accgaatgct ttttcggtta tgaaatccag gctgccgcga ccattggccg
     5041 ccgctttact atccatcacg gttacgccgt agtgatcaat aaaaacgtgg tagcggggga
     5101 tgatttcacc attcgccacg gcgtcactat cggcaatcgt ggtgccgata acatggcatg
     5161 tccacacatt ggcaacggcg tcgaactcgg tgccaacgtc attattcttg gtgatatcac
     5221 gcttggtaac aacgtcaccg tgggcgcggg cagcgtggtg ctcgactctg tcccggacaa
     5281 cgcgctggtg gtgggagaaa aagcgcgagt gaaggtaatt aaatgaatat tctgcaattt
     5341 aatgtgcgac tggcggaagg cggggcggca ggtgtggcgt tagatctcca ccagcgtgcg
     5401 ctgcaacagg ggctggcgtc acattttgtc tacggctacg gcaaaggcgg caaagagagc
     5461 gtcagccatc agaactatcc gcaggtcatc aaacatacgc cgcggatgac cgcaatggcg
     5521 aatattgccc tgtttcgtct gtttaatcgc gatctgtttg gcaatttcaa tgagttatat
     5581 cgcaccatta ctcgtacacc gggtccggtg gtcctgcatt ttcatgtgct gcacagctac
     5641 tggctaaatc ttaagggcgt ggtgcgcttt tgcgaaaagg tgaaaaacca caaaccggat
     5701 gtcactctgg tctggacgct gcacgaccac tggagcgtta ccgggcgctg cgcctttacc
     5761 gacggttgtg aaggctggaa aacgggctgc cagaaatgcc cgaccttaaa taactatccg
     5821 ccggtgaaga ttgatcgggc gcaccagctg gtggcgggca aacgccagtt attccgtgag
     5881 atgctggcgc tgggctgtca gtttatttcc cccagccagc atgtggctga cgctttcaat
     5941 agcctgtacg gtccagggcg ttgccggatt atcaataacg gtattgatat ggcaaccgag
     6001 gcgattctgg cggatttgcc tccggtgcgc gaaactcagg gcaagccgaa aatcgcggtg
     6061 gtagcgcatg acctgcgtta cgacggcaaa actaaccagc aactggtacg cgagatgatg
     6121 gcgctgggcg acaaaatcga actgcatacc tttggtaagt tctcgccgtt caccgctggc
     6181 aacgtggtta atcacggctt tgaaactgac aagcgcaagt tgatgagcgc gctcaatcag
     6241 atggatgcgc tggtgttcag ttctcgcgtc gataactacc cgctgatttt gtgtgaggcg
     6301 ttatcaattg gcgtgccggt gattgccacc catagcgacg cggcgcgtga agtgttgcaa
     6361 aaatccggcg gtaaaaccgt cagcgaagaa gaggtgctgc aactggtcca gttaagcaaa
     6421 ccggaaatcg cgcaggcgtt atttggtacc acgctggctg agttcagcca acgcagccgc
     6481 gccgcctaca gtggacaaca gatgctggag gagtatgtca acttctatca gaatctgtag
     6541 ctacctgctg ctgccgctga tctacctgct ggttaacgtc aaaatcaccc agcttggcga
     6601 aagtttcccc atcaccatcg tcactttttt acctgtcttg ttactgctgt ttttagaacg
     6661 catcagcgtt aaaaaattga tgattgcctt agggatttgc gcgggactca cggcgtttaa
     6721 ctacctgttt ggtcagtcgc tggatgccag taaatacgtc acttcaacta tgctgtttgt
     6781 ctatatcgtg atcattattg gtatggtgtg gagtattcgc tttaaaacga tttcgccaca
     6841 caaccatcgc aagatattac gtttctttta tctggtggtc gggctggtgg tggcgctggc
     6901 ggcggtggag atggcgcaaa ttatcctcac cggcggcagc agtattatgg agtcgatttc
     6961 gaaatatctg atttacagca acagctatgt gctgaatttc atcaaattcg gcggcaagcg
     7021 cacgacagca ctttatttcg aaccggcatt tttcgctctg gcattaatct caatttggct
     7081 cagcatcaaa cagtttggta tcaaaacgcc taaaacagat gctatgattc tcgcagggat
     7141 aatattatcc ggatcgtttt caggggttat gacctttatc ctgttttatt tgctggagtg
     7201 ggcatttcaa tatctgaata aagaggcgat taagaaaaag ttaccgttag cattgatttc
     7261 tctggcagta ttcctggttg gtgtggtaat tgcatttcct tatatttcca cccgtctggg
     7321 cgatttaggt acggaaggat cgtcatcgta ttatcgtatt gtcggtccgc tggtgatggt
     7381 cggttattct ttgacccata ttgacggtgt agtcagattt ggctcacttt atgaatatgt
     7441 cgcatcattc ggaatattta acggtgcgga tgtcggaaaa accatagaca atggtttgta
     7501 tctgctgatt atttattttt cttggttcgc ggtgttttta tcgctgtggt acatggggaa
     7561 agtgataaaa atgatgatca acgcttttgg tgataaccgc aattttcgcg tgcaattata
     7621 tctttttact ccggtatcgc tgttttttac cggttcgata tttagcccgg aatatgcatt
     7681 tttaatcgtc tgtccgttta ttttgcgaaa agcgttaaat attacgaggt aagaataaga
     7741 acatgttgct tagcataatc actgtcgcgt ttcgtaacct cgaagggata gtcaaaacac
     7801 atgcctcgct ggcgcatctg gcgcaggcgg aagatatcag cttcgaatgg attgttgtcg
     7861 atggcggttc caatgacggc actcgtgagt acctggaaaa tctcaatggt atctataacc
     7921 tacgctttgt cagcgagcca gataacggta tctacgacgc catgaacaag ggcattgcga
     7981 tggcacaagg caagttcgcg ttgtttctca actcgggcga tatttttcat caggatgccg
     8041 catattttgt ccgtaagtta aaaatgcaaa aagataacgt gatgatcacc ggcgatgcgc
     8101 tgctggattt tggcgacggg cataaaatta aacgtagcgc caaaccgggc tggtatattt
     8161 atcacagcct gcccgccagt catcaggcga tatttttccc ggtatccggt ttgaaaaaat
     8221 ggcgttatga cctggaatat aaagtttctt ctgattacgc gctggcagcc aaaatgtata
     8281 aagcaggtta tgcatttaaa aaactcaatg gcctggtgtc tgaattttct atgggtgggg
     8341 tatctaccac caataatatg gaattgtgtg ctgacgcgaa aaaagtccaa cggcaaatat
     8401 tacatgtgcc tggcttttgg gttgaattat cctggcattt acgccagcgt actacctcaa
     8461 agacgaaagc cttatataac aaaagctgaa tataagggaa aacgatgcag gatttaagcg
     8521 gtttctcggt gccgaaaggg ttccggggcg gcaacgctat taaagtgcaa ttgtggtggg
     8581 cagtacaggc aacaatattt gcctggtcgc cacaagtatt gtatcgctgg cgggcatttt
     8641 tattacgttt attcggcgca aaaataggaa aaaacgtagt tattcgtccg tcagtaaaaa
     8701 ttacctatcc gtggaaatta accttaggtg attacgtgtg ggtcggcgat gacgtcaatt
     8761 tatataccct cggtgaaata accattggcg cacattcggt gatatcgcaa aaaagttatt
     8821 tatgcaccgg gagccacgac catgcaagtc aacatttcac cattaacgcc acgcctattg
     8881 tgattggcga gaaatgctgg ctggcaaccg atgtttttgt tgccccaggc gtcacgattg
     8941 gcgacggcac cgtcgtgggt gcgcgaagca gtgtttttaa atcgcttccg gcaaatgtgg
     9001 tttgccgggg gaatcccgca gtggtgatac gcaaacgcgt tgaaactgaa taaattcaaa
     9061 aaaaatacag aggaataaga catgtcaaaa gtcgctctca tcaccggtgt aaccggacaa
     9121 gacggttctt acctggcaga gtttctgctg gaaaaaggtt acgaggtgca tggtattaag
     9181 cgtcgcgcat cgtcattcaa caccgagcgc gtggatcaca tttatcagga tccgcacacc
     9241 tgcaacccga aattccatct gcattatggc gacctgagtg atacctccaa cctgacgcgt
     9301 attttgcgtg aagtgcagcc ggatgaagtg tacaacctgg gcgcaatgag ccacgttgcg
     9361 gtttcttttg agtcaccgga atataccgct gacgtcgacg cgatgggtac gctgcgcctg
     9421 ctggaggcga tccgcttcct cggtctggaa aagaaaaccc gtttctatca ggcttccacc
     9481 tctgaactgt atggtctggt gcaggaaatt ccgcagaaag agaccacgcc gttctatccg
     9541 cgctctccgt atgcggtcgc caaactgtat gcctactgga tcaccgtcaa ctaccgtgaa
     9601 tcctacggca tgtatgcctg taacggtatt ctcttcaacc atgaatcccc gcgccgcggc
     9661 gaaaccttcg ttacccgcaa aatcacccgc gcaatcgcca acatcgccca ggggctggag
     9721 tcgtgcctgt acctcggcaa tatggattcc ctgcgtgact ggggccatgc caaagactac
     9781 gtaaaaatgc agtggatgat gctgcaacag gaacagccag aagatttcgt tatcgcgacc
     9841 ggcgttcagt actccgtacg tcagttcgta gaaatggcgg cagcacagct gggcatcaaa
     9901 ctgcgctttg aaggcacggg cgttgaagag aagggcattg tggtttccgt caccgggcat
     9961 gacgcgccgg gtgttaaacc gggtgatgtg attatcgccg ttgatccgcg ttacttccgt
    10021 ccggctgaag ttgaaacgct gctcggcgac ccgaccaaag cgcacgaaaa actgggctgg
    10081 aaaccggaaa tcaccctcag agagatggtg tctgaaatgg tggctaatga cctcgaagcg
    10141 gcgaaaaaac actctctgct gaaatctcac ggctacgacg tggcgatcgc gctggagtca
    10201 taagtatgag tagacaacgc atttttattg ctggccatcg cgggatggtc ggttccgcca
    10261 tcaggcggca gctcgaacag cgcggtgatg tggaactggt attacgcacc cgcgacgagc
    10321 tgaacctgct ggacagccgc gccgtgcatg atttctttgc cagcgaacgt attgaccagg
    10381 tctatctggc ggccgcgaaa gtgggcggca ttgttgccaa caacacgtat ccggcggatt
    10441 tcatctatca gaacatgatg attgagagca acatcattca cgccgcgcat cagaacgacg
    10501 tgaacaaact gctgtttctc ggatcgtcct gcatctaccc gaaactggca aaacagccga
    10561 tggcagaaag cgagttattg cagggcacgc tggagccgac caacgagcct tacgccattg
    10621 ccaaaatcgc cgggatcaaa ctgtgcgaat catacaaccg ccagtacgga cgcgattacc
    10681 gctcagtcat gccgaccaac ctgtatggcc cgcatgacaa cttccacccg agtaattcgc
    10741 atgtgatccc tgcattgctg cgtcgcttcc acgaggcgac ggcacagaat gcaccggacg
    10801 tggtggtgtg gggcagcggt acgccgatgc gagaatttct gcacgtcgat gatatggcgg
    10861 cggcgagcat tcatgtcatg gagctggcgc atgaagtctg gctggagaac acccagccga
    10921 tgctgtcgca cattaacgtc ggcacgggcg ttgactgcac tatccgcgag ctggcgcaaa
    10981 ccatcgccaa agtggtgggt tacaaaggtc gggtggtttt tgatgccagc aaaccggatg
    11041 gtacgccgcg caaactgctg gatgtgacgc gcctgcatca gcttggctgg tatcacgaaa
    11101 tctcactgga agcggggctt gccagcactt accagtggtt ccttgagaat caagaccgct
    11161 ttcgggggta atgatgtttt tacgtcagga agactttgcc acggtagtgc gctccacacc
    11221 gcttgtctct ctcgacttta ttgtcgagaa cagtcgcggc gagtttctgc ttggcaaaag
    11281 aaccaaccgc ccggcgcagg gttactggtt tgtgccggga gggcgcgtgc agaaagacga
    11341 aacgctggaa gccgcatttg agcggctgac gatggcggaa ctggggctgc gtctgccgat
    11401 aacagcaggc cagttttacg gtgtctggca gcacttttat gacgataact tctccggcac
    11461 ggatttcacc actcactatg tggtgctcgg ttttcgcttc agagtagcgg aagaagaact
    11521 gttactgccg gatgagcagc atgacgatta ccgctggctg acgccggacg cgctgctcgc
    11581 cagtaacgat gttcacgcta acagccgcgc ctattttctc gctgagaagc gtgccggagt
    11641 acccggatta tgaaaatact ggtctacggc attaactact cgccggagtt aaccggcatc
    11701 ggcaaataca ccggcgagat ggtggaatgg ctggcggcgc aaggtcatga ggtgcgggtt
    11761 attaccgcac cgccttacta cccgcagtgg caagtgggcg agaactattc cgcctggcgg
    11821 tacaaacgag aagagggggc cgctacggtg tggcgctgcc cgctgtacgt gccaaaacag
    11881 ccgagcaccc tgaaacgcct gttgcatctc ggcagttttg ccgtcagcag ttttttcccg
    11941 ttgatggcgc aacgtcgctg gaagccggat cgcattatcg gcgtagtgcc aacgctgttt
    12001 tgcacgccgg gaatgcgcct gctggcgaaa ctctctggcg cgcgtaccgt gctgcatatt
    12061 caggattacg aagtagatgc catgctgggg ctgggccttg ccggaaaagg caaaggcggc
    12121 aaagtggcac agctggcaac ggcgttcgaa cgtagcggac tgcataacgt cgataacgtt
    12181 tccacgattt cgcgttcgat gatgaataaa gccatcgaaa aaggcgtggc ggcggaaaac
    12241 gtcatcttct tccccaactg gtcggaaatc gcccgttttc agcatgttgc agacgccgat
    12301 gttgatgccc ttcgtaacca gcttggcctg ccggataaca aaaaaatcat tctttactcc
    12361 ggcaatattg gtgaaaagca ggggctggaa aacgttattg aagcagccga tcgcctgcgc
    12421 gatgaaccgc tgatttttgc cattgtcggg cagggcggcg gcaaagcgcg gctggaaaaa
    12481 atggcgcagc agcgtggact gcgcaacatg caatttttcc cgctgcaatc gtatgacgct
    12541 ttacccgcac tgctgaagat gggcgattgc catctggtgg tgcaaaaacg cggcgcggca
    12601 gatgccgtat tgccgtcgaa actgaccaat attctggcag taggcggtaa cgcggtgatt
    12661 actgctgaag cccacacaga actgggacag ctttgcgaaa cctttccggg cattgcggtt
    12721 tgcgtagaac cggaatcggt tgaggcgctg gtggcgggga ttcgtcaggc gctcctgctg
    12781 cccaaacaca acacggtggc acgtgaatat gccgaacgca cgcttgataa agagaacgtg
    12841 ttacgtcaat ttataaatga tattcgggga taattatggc gcagtcgaaa ctctatccag
    12901 ttgtgatggc aggtggctcc ggtagccgct tatggccgct ttcccgcgta ctttatccca
    12961 agcagttttt atgcctgaaa ggcgatctca ccatgctgca aaccaccatc tgccgcctga
    13021 acggcgtgga gtgcgaaagc cctgtggtga tttgcaatga gcagcaccgc tttattgttg
    13081 cggaacaact gcgtcaactg aacaaactca ctgagaacat tattctcgaa ccggcagggc
    13141 gtaacacagc accggccatt gcgctggcgg cgctggcggc aaaacgtcat agcccggaga
    13201 acgacccatt aatgctggtg ctggcggcgg atcatgtgat tgccgatgaa gacgcattcc
    13261 gcgccgccgt gcgtaacgct atgccgtatg ccgaagcggg caagctggtg accttcggca
    13321 ttgtgccgga tctacctgaa accggatatg gctatattcg tcgcggtgaa gtgtcggcgg
    13381 gtgagcagga tacggtggcc tttgaagtgg cgcagtttgt cgaaaaacca aatctggaaa
    13441 ccgcgcaggc ctatgtggca agcggcgaat attactggaa cagcggtatg ttcctgttcc
    13501 gtgccggacg ctatctcgaa gaactgaaaa aatatcgccc ggatattctc gacgcctgtg
    13561 aaaaagcgat gagcgccgtc gatccggatc tcgattttat tcgcgtggat gaagacgcgt
    13621 ttctggcctg cccggaagag tcggtggatt acgcggtcat ggaacgtacg gcagatgccg
    13681 ttgtggtgcc gatggatgcg ggctggagcg atgtcggctc ctggtcctca ttatgggaga
    13741 tcagcgccca caccgccgag ggcaacgttt gccacggcga tgtgattaat cacaaaactg
    13801 aaaacagcta tgtgtatgcc gaatctggcc tggtcaccac cgtcggggtg aaagatttgg
    13861 tggtagtgca gaccaaagat gcggtgctga ttgccgaccg taacgcggtg caggatgtga
    13921 aaaaagtggt cgagcagatc aaagccgatg gtcgccatga gcatcgggtg catcgcgaag
    13981 tctatcgccc gtggggcaaa tatgactcta tcgacgcggg cgaccgctac caggtgaaac
    14041 gcatcaccgt gaaaccgggc gaaggcttgt cggtacagat gcaccatcac cgcgcggaac
    14101 actgggtggt tgtcgcggga acggcaaaag tcaccattga cggtgatatc aaactgcttg
    14161 gtgaaaacga gtcgatttat attccgctgg gggcgacgca ctgcctggaa aacccgggga
    14221 aaattccgct cgatttaatt gaagtgcgct ccggctctta tctcgaagag gatgatgtgg
    14281 tgcgcttcgc ggatcgctac ggacgggtgt aaacgtcgca tcaggcgctg attgtcggat
    14341 gcggcgtgaa cgccttatcc gacctacggt tcggttttgt aggcctgata agacgcggca
    14401 gcgtcgcatc aggcaatgaa tgcgaaaccg cggtgtaaat aacgacaaaa acaaaattgg
    14461 ccgtttcggt cagggccaac tattgcctga aaaagggtaa cgatatgaaa aaattaacct
    14521 gctttaaagc ctatgatatt cgcgggaaat taggcgaaga actgaatgaa gatattgcct
    14581 ggcgcattgg tcgtgcctat ggcgaatttc tcaaaccgaa aaccattgtg ttaggcggtg
    14641 atgcccgtct caccagcgaa accttaaaac tggcgctggc aaaaggttta caggatgcgg
    14701 gcgttgacgt gctggatatt ggtatgtccg gcaccgaaga gatctatttc gccacgttcc
    14761 atctcggcgt ggatggcggc attgaagtta ctgccagcca taacccgatg gattacaacg
    14821 gcatgaaact ggtgcgcgaa ggggctcgcc cgatcagcgg cgataccgga ctgcgcgatg
    14881 tccagcgtct ggcagaagcc aacgactttc ctcccgttga tgaaaccaaa cgcggtcgct
    14941 atcagcaaat caatctgcgt gacgcttacg ttgatcacct gttcggttat atcaacgtca
    15001 aaaaccttac gccgctcaag ctggtgatca actccgggaa cggcgcggcg ggtccggtgg
    15061 tggacgccat cgaagcccgc tttaaagccc tcggcgcacc ggtggaattg atcaaagtgc
    15121 acaacacgcc cgacggcaat ttccccaacg gtattcctaa cccgctgctg ccggaatgtc
    15181 gcgacgacac ccgcaatgcg gtcattaaac acggcgcgga tatgggcatt gcttttgatg
    15241 gcgattttga ccgctgtttc ctgtttgacg aaaaagggca gtttatcgag ggctactaca
    15301 ttgtcggtct gctggcagaa gcgttcctcg aaaaaaatcc cggcgcgaag atcatccacg
    15361 atccacgtct ctcctggaat accgttgatg tggtgactgc cgcaggcggc accccggtaa
    15421 tgtcgaaaac cggacacgcc tttattaaag aacggatgcg caaggaagac gccatctacg
    15481 gtggcgaaat gagcgcccac cattacttcc gtgattttgc ttactgcgac agcggcatga
    15541 tcccgtggct gctggtcgcc gaactggtgt gcctgaaagg aaagacgctg ggcgaactgg
    15601 tgcgcgaccg gatggcagcg tttccgtcaa gcggtgagat caacagcaaa ctggcgcaac
    15661 ccgttgaggc gattaaccgc gtggaacagc attttagccg tgaggcgctg gcggtggatc
    15721 gcaccgatgg catcagcatg acctttgccg actggcgctt taatctgcgc tcctccaaca
    15781 ccgaaccggt ggtgcggttg aatgtggaat cacgcggtga tgtgccgctg atggaagcgc
    15841 gaacgcgaac tctgctgacg ttgctgaacg agtaatgtcg gatcttccct taccccattg
    15901 cgggtaaggg gctaataaca ggaacaacga tgacaaatct aaaaaagcgc gagcgagcga
    15961 aaaccaatgc atcgttaatc tctatggtgc aacgcttttc agatatcacc atcatgtttg
    16021 ccggactatg gctggtttgc gaagtgagcg gactgtcatt cctctacatg cacctgttgg
    16081 tggcgctgat tacgctggtg gtgttccaga tgctgggcgg catcaccgat ttttatcgct
    16141 catggcgcgg tgttcgggca gcgacagaat ttgccctgtt gctacaaaac tggaccttaa
    16201 gcgtgatttt cagcgccgga ctggtggcgt tcaacaatga tttcgacacg caactgaaaa
    16261 tctggctggc gtggtatggg ctgaccagta tcggactggt ggtttgccgt tcatgtattc
    16321 gcattggggc gggctggctg cgtaatcatg gctataacaa gcgcatggtc gcggtggcgg
    16381 gggatttagc cgccgggcaa atgctgatgg agagcttccg taaccagccg tggttagggt
    16441 ttgaagtggt gggcgtttac cacgacccga aactgggcgg cgtttctaac gactgggcgg
    16501 gtaacctgca acagctggtc gaggatgcta aagcaggcaa gattcataac gtctatatcg
    16561 cgatgcaaat gtgcgacggc gcgcgagtga aaaaactggt ccatcaactg gcggacacca
    16621 cctgttcggt gctgctgatc cccgatgtct ttaccttcaa cattctccat tcacgtcttg
    16681 aagagatgaa cggcgttccg gtggtgccgc tttacgacac gccgctttcc ggggttaacc
    16741 gcctgctcaa acgtgcggaa gacattgtgc tggcgacgct tatcctgctg ctgatctccc
    16801 cggtgctgtg ctgcattgcg ctggcggtga aactcagttc acctgggccg gttattttcc
    16861 gccagactcg ctacggcatg gatggcaagc cgatcaaagt gtggaagttc cgttccatga
    16921 aagtgatgga gaacgacaaa gtggtgaccc aggcgacgca gaacgatccg cgcgtcacca
    16981 aagtggggaa ctttctgcgc cgcacctcgc tggatgaatt gccgcagttt atcaatgtgc
    17041 tgaccggggg gatgtcgatt gtcggtccac gtccgcacgc ggtggcgcat aacgaacagt
    17101 atcgacagct cattgaaggc tacatgctgc gtcataaggt gaaaccgggc attaccggct
    17161 gggcgcagat taacggctgg cgcggcgaaa ccgacacgct ggagaaaatg gaaaaacgcg
    17221 tcgagttcga ccttgagtac atccgcgaat ggagcgtctg gttcgatatc aaaatcgttt
    17281 tcctgacggt attcaaagga ttcgttaaca aagcggcata ttgatatgag cttacgtgaa
    17341 aaaaccatca gcggcgcgaa gtggtcggcg attgccacgg tgatcatcat cggcctcggg
    17401 ctggtgcaga tgaccgtgct ggcgcggatt atcgacaacc accagttcgg tttgcttacc
    17461 gtgtcgctgg tgatcatcgc gctggcagat acgctttctg acttcggtat tgctaactcg
    17521 attattcagc gaaaagaaat cagccacctt gaactcacca cgctgtactg gctgaacgtt
    17581 gggctggggc ttgtggtgtg tgtggcggtg tttttgttga gtgatctcat tggtgacgtg
    17641 ctgaacaacc cggacctggc accgttgatt aaaacattgt cgctggcgtt tgtggttatc
    17701 ccccacgggc aacagttccg cgcgttgatg caaaaggagc tggagttcaa caaaatcggc
    17761 atgatcgaaa ccagcgcggt actggcgggc ttcactttta cggtggttag cgcccatttc
    17821 tggccgctgg cgatgaccgc gatcctcggt tatctggtca atagtgcggt gagaacgctg
    17881 ctgtttggct actttggccg caaaatttat cgccccggtc tgcatttctc gctggcgtct
    17941 gttgcgccga acttacgctt tggtgcctgg ctgacggcgg acagcatcat caactatctc
    18001 aataccaacc tttcaacgct ggtgctggcg cgtattctcg gcgcgggcgt ggcaggggga
    18061 tacaacctgg cgtacaacgt ggccgttgtg ccgccgatga agctgaaccc gatcatcacc
    18121 cgtgtgttgt ttccggcatt cgccaaaatt caggacgata ccgaaaagct gcgcgtcaac
    18181 ttctacaagc tgctgtcggt ggtggggatt atcaactttc cggcgctgct cgggctgatg
    18241 gtggtgtcaa ataactttgt accgctggta tttggcgaaa agtggaacag cattattccg
    18301 gtgctgcaat tgctgtgtgt ggtgggtctg ctgcgctccg taggtaaccc gattggttcg
    18361 ctgctgatgg cgaaagcgcg ggtcgatatc agctttaaat tcaacgtatt caaaacattt
    18421 ctgtttattc cggcgattgt tattggtggg cagatggcgg gcgcaatcgg cgtcacgctc
    18481 ggtttcctgc tggtgcagat tatcaacacc attctgagtt acttcgtgat gattaaaccg
    18541 gttcttggtt ccagttatcg ccagtacatc ctgagtttgt ggctgccgtt ttatctctcg
    18601 ctgccgacgc tggtggtcag ttatgtgctg ggcattgtgc tgaaagggca actggcgctg
    18661 gggatgctgc tggcggtgca aatagccgcg ggcgtgctgg cgtttgtggt gatgattgtg
    18721 ctgtcgcgcc atccgctggt ggtggaagtg aagcgccagt tttgtcgcag cgaaaaaatg
    18781 aaaatgcttt tacgggcggg gtgagttgtt gttttcggtt ccgacaacgg ttgccgggtg
    18841 cagcgtaaaa caccttatcc atcctacggt tctgtgctcg ggtcttgtag gcctgataag
    18901 acgcgccagc gtcgcatcag gcaaaaaacg gcaacgaaca caacaaacac agcctcacct
    18961 ggcatcaaat ttatgaggaa aaaatgaaat tacttattct gggcaaccac acttgcggca
    19021 atcgtggcga cagcgccatc ttgcgcggct tacttgatgc catcaacatc ctcaatccac
    19081 ataccgaagt ggacgtgatg agccgctatc cggtcagttc ttcctggctg ctcaaccgcc
    19141 cggtaatggg tgatccgctg ttccttcaaa tgaaacaaca caacagcgcg gcgggcgttg
    19201 tcgggcgcgt taaaaaagtc ctccgtcgcc gctatcagca ccaggtattg ctctcacgcg
    19261 tcaccgacac tggcaagctg cgcaatatcg ccatcgccca gggattcacc gacttcgtgc
    19321 gcctactgtc aggttacgac gccattattc aggtcggcgg atcgtttttt gtcgatctct
    19381 acggcgtgcc gcagtttgaa catgcacttt gcacatttat ggcgaaaaag ccgctgttta
    19441 tgattggtca cagcgtcggt cccttccagg atgagcaatt taaccaactg gcgaactacg
    19501 tttttggtca ctgcgacgcg ctgatcttgc gcgaatcggt cagcctcgat ctaatgaaac
    19561 gcagcaatat caccactgca aaagttgaac atggcgtcga taccgcgtgg ctggtcgatc
    19621 accacacaga agacttcacc gccagctatg ccgtccaaca ctggctggac gttgccgcac
    19681 aacagaaaac ggtggcaatt accctgcgcg aactggcacc gttcgacaaa cgcctcggca
    19741 ccactcagca agtgtatgaa aaagcctttg ccggggtggt caatcgcatt ctcgacgaag
    19801 gctatcaggt cattgcgctc tccacctgta cgggtatcga cagctataac aaagacgacc
    19861 gcatggtggc gctcaacctg cgccagcaca tcagcgatcc tgcccgttat cacgtagtga
    19921 tggatgaact taacgatctg gaaatgggca aaattctcgg tgcctgtgaa ctcaccgtcg
    19981 gtacgcgcct gcactccgcc attatctcaa tgaactttgc tactccggcg attgccatca
    20041 actatgaaca taaatccgcc ggggttatgc agcagctggg actaccggag atggcaattg
    20101 atatccgtca tttattagac ggcagcctgc aagcgatggt tgcggatacc ttaggccagc
    20161 ttccggcgct gaacgcacga cttagcgaag ccgtcagtcg tgagcgtcag acgggaatgc
    20221 agatggtgca gtccgtactg gaacgcatcg gggaggtgaa atgaaggtcg gcttcttctt
    20281 attgaaattt ccgctgtcgt cagaaacctt cgttcttaac caaatcactg cgtttattga
    20341 tatgggcttt gaagtggaga ttgtcgcgct gcaaaaaggc gacacacaaa acacccacgt
    20401 ggcatggact aaatacaacc ttgccgccag aacccgctgg ttacaggacg aacctacggg
    20461 caaagtggcg aaactgagcc accgagccag tcagaccttg cgcggcattc atcgtaaaaa
    20521 tacctggcag gcgctcaacc tcaaacgcta tggtgccgag tcgtggaacc tgattttgtc
    20581 tgccatttgc ggccaggtcg caacaccgtt tcgcgccgat gtgttcatcg ctcattttgg
    20641 ccctgcgggg gtaaccgcag caaaactacg cgaactgggt gtcattcacg gcaaaattgc
    20701 cactattttc cacggcattg atatttccag tcgggaagtg ctcaaccatt acactcccga
    20761 atatcaacaa ctgtttcgac gtggcgacct gatgctaccg ataagcgatc tgtgggccgg
    20821 aaggctgcaa aaaatgggct gcccgaggga aaaaatcgcc gtatcgcgca tgggcgtaga
    20881 tatgacgcgc tttagcccgc gtcccgtgaa agcgcccgca acaccgctgg agattatttc
    20941 cgtcgcacgc ttaaccgaga aaaaaggtct gcatgtggcg attgaagcct gccggcagtt
    21001 gaaagagcag ggcgtggcat ttcgctatcg catcctcggc attggcccgt gggaacgacg
    21061 cctgcgcacg ctcatcgaac aatatcaact ggaagatgtg gtggagatgc cgggctttaa
    21121 accgagccat gaagtgaaag cgatgctcga cgacgcggat gtcttcctgt tgccatcggt
    21181 tacaggtgcg gatggcgata tggaaggcat tccggtggcg ctgatggagg cgatggcggt
    21241 cggcattccg gtggtttcta ctctgcatag cggaataccg gaactggtgg aggctgacaa
    21301 atccggctgg ctggtgcctg agaacgatgc tcgcgcactg gcgcaacgac tggcggcgtt
    21361 tagccaactg gacaccgacg aattggctcc ggtcgtcaaa cgcgcgcgcg aaaaagttga
    21421 acacgatttt aaccagcagg tgatcaatcg agaactcgcc agcttgctgc aggctttata
    21481 gaggagaacg tatgccattt aaaaaactct cccgacgcac cttcctgacg gcaagctcgg
    21541 cgcttgcctt ccttcatacc cctttcgctc gcgcatttcc cgcccgacaa agcgttaata
    21601 tcaacgacta caaccctcac gactggatcg cctcatttaa acaagccttc agcgaagggc
    21661 aaacggttgt cgtgcctgct ggattcgttt gtgacaatat caacaccggc atcttcattc
    21721 ctcctggcaa aacgttacac atccttggaa gcctgcgcgg caacggcaga gggcgatttg
    21781 tcttacagga cggcagccag gtgacagggg aggagggcgg cagtatgcat aacatcaccc
    21841 tggatgtgcg tggttctgac tgcaccatca aagggctggc gatgagcggc tttggtccgg
    21901 taacgcagat ttatatcggc ggcaaaaaca aacgggtcat gcgcaacctg accatcgata
    21961 acctgaccat tagccacgct aattacgcca tcttacgcca gggatttcat aaccagatta
    22021 tcggtgccaa catcaccaat tgtaagttca gcgacttaca gggcgatgcc attgaatgga
    22081 acgtggcgat taacgaccgc gatattttga tctccgacca tgtcatcgag cgcatcaact
    22141 gtactaacgg caaaatcaac tggggcattg gcataggcct tgcgggaagc acttacgata
    22201 ataattaccc ggaagatcag gcagtgaaaa actttgtcgt ggcgaatatc acgggatcgg
    22261 attgtcggca gttgatacat gttgaaaatg gtaaacattt tgttattcgt aatatcaaag
    22321 cccgcaatat cacgcaggat ttcagtaaga aagcaggtat tgataacgcc acagtcgcta
    22381 tttacggttg tgacaatttc gtgattgata atattgaaat gattaatagc gccgggatgt
    22441 taatcggcta tggggtaatt aaaggcaatt atctctcgat tccgcaaaat ttccgagtga
    22501 ataatattca actggataac acccatcttg cttataaatt gcgcggcctc caaatctctg
    22561 ccgggaatgc cgtctccttt gtggcactaa ctaacattga gatgaagcgt gcgtcgctgg
    22621 agttacacaa caaaccgcaa catctttttc tgcgtaatat caaagtgatg caggaatcct
    22681 ctgttggacc cgcattgatt atgaacttcg acatgcgcaa agacgttcga ggcgtcttta
    22741 tggcgaaaga agaaacactg ctgtctcttg caaatgttca tgcggtgaat gagaaaggac
    22801 aaagctccgt cgatatcgac aggattaatc accatattgt taatgtggaa aagattaact
    22861 ttagattgcc ggaacggaga gagtagattt gcgaccattc ataaacttac tggatataat
    22921 gttctaaaag attataatcc tcacactaac tgttaaagct ggggctgggc agcattgact
    22981 actcagggaa ggtactcatc tttatcttgt gtttcttacg taacaaccag actaaagtga
    23041 tttatgaacg ataacgtttt gctcatagga gcttccggat tcgtaggaac ccgactactt
    23101 gaaacggcaa ttgctgactt taatatcaag aacctggaca aacagcagag ccacttttat
    23161 ccagaaatca cacagattgg tgatgttcgt gatcaacagg cactcgacca ggcgttagcc
    23221 ggttttgaca ctgttgtact actggcagcg gaacaccgcg atgacgtcag ccctacttct
    23281 ctctattatg atgtcaacgt tcagggtacc cgcaatgtgc tggcggccat ggaaaaaaat
    23341 ggcgttaaaa atatcatctt taccagttcc gttgctgttt atggtttgaa caaacacaac
    23401 cctgacgaaa accatccaca cgaccctttc aaccactacg gcaaaagcaa gtggcaggcg
    23461 gaggaagtgc tgcgtgaatg gtataacaaa gcaccaacag aacgttcatt aactatcatc
    23521 cgtcctaccg ttatcttcgg tgaacgcaac cgcggtaacg tctataactt gctgaaacag
    23581 atcgctggcg gcaagtttat gatggtgggc gcagggacta actataagtc catggcttat
    23641 gttggaaaca ttgttgagtt tatcaagtac aaactgaaga atgttgccgc aggttacgag
    23701 gtttataact acgttgataa gccagacctg aacatgaacc agttggttgc tgaagttgaa
    23761 caaagcctga acaaaaagat cccttctatg cacttgcctt acccactagg aatgctgggt
    23821 ggatattgct ttgatatcct gagcaaaatt acgggcaaaa aatacgctgt cagctctgtg
    23881 cgcgtgaaaa aattctgcgc aacaacacag tttgacgcaa cgaaagtgca ttcttcaggt
    23941 tttgtggcac cgtatacgct gtcgcaaggt ctggatcgaa ctctgcagta tgaattcgtc
    24001 catgccaaaa aagacgacat aacgtttgtt tctgagtaat gcagacgagt cagagaaaaa
    24061 aggaccaggt tggactacga ttattcggaa agaggtactg attaaattat cagtatgtgg
    24121 atttccgagt aatccgagta acccagatgc tgatggtgtg tagtgttatc gtaaggttta
    24181 atcaaagtta tgcggaacta agctttggta tatagccata acgtttggct aacctatggc
    24241 aataatttac gcactatcag ccacttagag gtaggaaaag aatgacgaat ttaaaagcag
    24301 ttataccggt agcaggtctt gggatgcata tgttgcctgc cactaaggcg attcccaaag
    24361 agatgctacc gatcgtcgac aagccaatga ttcagtacat tgttgacgag attgtggctg
    24421 cagggatcaa agaaatcctc ctggtaactc acgcgtccaa gaacgcagtc gaaaaccact
    24481 tcgacacctc ttatgaatta gaatctctcc ttgaacagcg cgtgaagcgt caactactgg
    24541 cggaagtaca atctatctgt ccgccgggcg tgaccattat gaacgtgcgt cagggtgaac
    24601 ctttaggttt aggccactcc attttatgtg cgcgacctgc cattggtgac aatccatttg
    24661 tcgtggtgct gccagacgtt gtgatcgacg acgccagcgc cgacccgcta cgctacaacc
    24721 ttgctgccat gattgcgcgc ttcaatgaaa cgggacgcag ccaggtgctg gcaaaacgta
    24781 tgccgggcga cctctctgaa tactccgtca tccagaccaa agaaccgctg gatcgtgaag
    24841 gcaaagtcag ccgcattgtt gaatttatcg aaaagccgga tcagccgcag acgctggact
    24901 cagacatcat ggccgttggt cgatatgtgc tttctgccga tatttggccg gaactggaac
    24961 gtactcagcc tggtgcatgg ggacgtattc agctgactga tgccattgct gagctggcaa
    25021 aaaaacaatc cgttgatgca atgctgatga ctggcgacag ttacgactgc ggtaaaaaaa
    25081 tgggctatat gcaggcgttt gtgaagtatg gactacgcaa cttgaaagaa ggggcgaagt
    25141 tccgcaaagg tatcgagaag ctgttaagcg aataatgaaa atctgaccga atgtaacggt
    25201 tgataaggaa ataatatcgg cagtgaaaaa ctatagatca ataaatatca ctgctgttga
    25261 gcttttataa ttaact
//
LOCUS       DBFIFM010000050        25107 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.050, whole genome shotgun sequence.
ACCESSION   DBFIFM010000050 DBFIFM010000000
VERSION     DBFIFM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.050"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(128..493)
                     /gene="yhaH"
                     /locus_tag="N7C74_001797"
     CDS             complement(128..493)
                     /gene="yhaH"
                     /locus_tag="N7C74_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGU2431678.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(785..1771)
                     /gene="yqjG"
                     /locus_tag="N7C74_001798"
     CDS             complement(785..1771)
                     /gene="yqjG"
                     /locus_tag="N7C74_001798"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HGU2431679.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVEKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTRIISIGPWQDLDEPHGRDVRLG"
     gene            complement(1841..2233)
                     /gene="yqjF"
                     /locus_tag="N7C74_001799"
     CDS             complement(1841..2233)
                     /gene="yqjF"
                     /locus_tag="N7C74_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HGU2431680.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(2419..2718)
                     /gene="yqjK"
                     /locus_tag="N7C74_001800"
     CDS             complement(2419..2718)
                     /gene="yqjK"
                     /locus_tag="N7C74_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HGU2431681.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(2708..3112)
                     /gene="yqjE"
                     /locus_tag="N7C74_001801"
     CDS             complement(2708..3112)
                     /gene="yqjE"
                     /locus_tag="N7C74_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGU2431682.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(3115..3420)
                     /gene="yqjD"
                     /locus_tag="N7C74_001802"
     CDS             complement(3115..3420)
                     /gene="yqjD"
                     /locus_tag="N7C74_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HGU2431683.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(3458..3826)
                     /gene="yqjC"
                     /locus_tag="N7C74_001803"
     CDS             complement(3458..3826)
                     /gene="yqjC"
                     /locus_tag="N7C74_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HGU2431684.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(3973..4356)
                     /gene="mzrA"
                     /locus_tag="N7C74_001804"
     CDS             complement(3973..4356)
                     /gene="mzrA"
                     /locus_tag="N7C74_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HGU2431685.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPQGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(4360..5022)
                     /gene="yqjA"
                     /locus_tag="N7C74_001805"
     CDS             complement(4360..5022)
                     /gene="yqjA"
                     /locus_tag="N7C74_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HGU2431686.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(5044..5127)
                     /gene="yqiM"
                     /locus_tag="N7C74_001806"
     CDS             complement(5044..5127)
                     /gene="yqiM"
                     /locus_tag="N7C74_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HGU2431687.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            complement(5367..6143)
                     /gene="exuR"
                     /locus_tag="N7C74_001807"
     CDS             complement(5367..6143)
                     /gene="exuR"
                     /locus_tag="N7C74_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HGU2431688.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(6273..7571)
                     /gene="exuT"
                     /locus_tag="N7C74_001808"
     CDS             complement(6273..7571)
                     /gene="exuT"
                     /locus_tag="N7C74_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HGU2431689.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            8054..9466
                     /gene="uxaC"
                     /locus_tag="N7C74_001809"
     CDS             8054..9466
                     /gene="uxaC"
                     /locus_tag="N7C74_001809"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HGU2431690.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMSGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            9481..10968
                     /gene="uxaA"
                     /locus_tag="N7C74_001810"
     CDS             9481..10968
                     /gene="uxaA"
                     /locus_tag="N7C74_001810"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HGU2431691.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVRLRQDVARGHKF
                     ALTNIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            11051..11602
                     /gene="ygjV"
                     /locus_tag="N7C74_001811"
     CDS             11051..11602
                     /gene="ygjV"
                     /locus_tag="N7C74_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HGU2431692.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(11607..12851)
                     /gene="sstT"
                     /locus_tag="N7C74_001812"
     CDS             complement(11607..12851)
                     /gene="sstT"
                     /locus_tag="N7C74_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HGU2431693.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(13250..14215)
                     /gene="alx"
                     /locus_tag="N7C74_001813"
     CDS             complement(13250..14215)
                     /gene="alx"
                     /locus_tag="N7C74_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HGU2431694.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQQVG"
     gene            complement(14499..15485)
                     /gene="ygjR"
                     /locus_tag="N7C74_001814"
     CDS             complement(14499..15485)
                     /gene="ygjR"
                     /locus_tag="N7C74_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU2431695.1"
                     /translation="MIRFAVIGTNWITHQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     EFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(15564..16256)
                     /gene="ygjQ"
                     /locus_tag="N7C74_001815"
     CDS             complement(15564..16256)
                     /gene="ygjQ"
                     /locus_tag="N7C74_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HGU2431696.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWSDVNVVPARNVGLLLGARPGNRYFTRRIDTAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRANKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLEKGRGKIVRLREKLARVSAVIDAKIFNRQPKYLGPSVII
                     GPFSEHGCPAQK"
     gene            complement(16333..16836)
                     /gene="ygjP"
                     /locus_tag="N7C74_001816"
     CDS             complement(16333..16836)
                     /gene="ygjP"
                     /locus_tag="N7C74_001816"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HGU2431697.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            16921..18057
                     /gene="rlmG"
                     /locus_tag="N7C74_001817"
     CDS             16921..18057
                     /gene="rlmG"
                     /locus_tag="N7C74_001817"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HGU2431698.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPQTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            18341..18655
                     /gene="higB"
                     /locus_tag="N7C74_001818"
     CDS             18341..18655
                     /gene="higB"
                     /locus_tag="N7C74_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HGU2431699.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            18652..19068
                     /gene="higA"
                     /locus_tag="N7C74_001819"
     CDS             18652..19068
                     /gene="higA"
                     /locus_tag="N7C74_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HGU2431700.1"
                     /translation="MIAIADILHAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(19113..21131)
                     /gene="fadH"
                     /locus_tag="N7C74_001820"
     CDS             complement(19113..21131)
                     /gene="fadH"
                     /locus_tag="N7C74_001820"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HGU2431701.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYHNRMRFAVEVVSAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAMQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRTPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(21657..24008)
                     /gene="ygjK"
                     /locus_tag="N7C74_001821"
     CDS             complement(21657..24008)
                     /gene="ygjK"
                     /locus_tag="N7C74_001821"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HGU2431702.1"
                     /translation="MKIKTILTPVTCALLISFSAHATNADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYNIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTSQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVASGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPATQ
                     YFYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHFYMLYNDFFRKQ"
     gene            complement(24025..25095)
                     /gene="ygjJ"
                     /locus_tag="N7C74_001822"
     CDS             complement(24025..25095)
                     /gene="ygjJ"
                     /locus_tag="N7C74_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HGU2431703.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASNDPSDQIFLMQYFYW"
BASE COUNT         6148 a         6620 c         6458 g         5881 t
ORIGIN      
        1 atggttattt ttataggtaa tttatataac gacattgaaa tattttaaat aaacattagt
       61 aaattattca ttcatcaaca tgatgataaa agccggatga gtcagacatc cggcgagcag
      121 atttgtttta aggttccagt tttggatcag gtccaaagcg gttttcgcct ggcgtacccg
      181 cctggcagtt aaagacgatg ataataagcc agccgataaa cgggattaag aatagcagtg
      241 cccaccacgc cgagcggtcg gtgtcgtgca ggcggcggaa ctgaactgcc caccacggta
      301 aaaacaccag aataccgtag attgtcgtca ggatgccttc gccaccagcg cgttgccagc
      361 ctaacatttt atccagcagc cccagcacga acgtaaagat gatgttgacc agaataaaca
      421 tccagtactc tttgcgccgc gcacgacccc ggaaaccgac ataatttttt agtactttca
      481 gataccagtc catttttgcc tcaattttgc tcaatcactt gtttttaaag cagcaaatta
      541 agaatagacg tgaatgaaat ggcagtaaat attcgttcaa ggaattaata aagtgctgtg
      601 aaggcgcgct aagactgatg acaagcgcaa aattgccaga tgcgctacgc ttatctggcc
      661 cgcgcggtca ttgcaacata ttgaatataa aagttttgta ggtttgataa ggcgttcatg
      721 ccgcatccga catgaacaac atgcacttag ctaattatca gaggcacctc gtaaggtgct
      781 ttttttaacc taagcgaaca tctcgtccat gcggttcatc gagatcctgc cacggaccaa
      841 ttgaaataat ccgcgtaggg ttgatggtct tatgactgcg gaagtaatga ttacggatat
      901 gatcgaaatt gactgtttcg gcaattcccg gcatctggta gatatcacgc aggaagccat
      961 acagattaag gtaatcgctg atgcggtgct tatcacactt gaagtgggtc acatacactg
     1021 gatcaaaacg gaccagcgtg gtccacaggc gaatatcggc ttcggttagc tggttgccgg
     1081 tcaggtaacg atgctgacct aaaatctgtt ccagtcgcgc cagcgattca aacactttct
     1141 ccaccgcttc gtcgtaggct tgctggctgg tggcaaaacc agctttatac acgccgttgt
     1201 taacggtgtc ataaatccag ccgttaagtt cgtcaatttt cgtttgcagg gctggtgggt
     1261 agtaatcacc cgctttcgcg cccagcgcat caaacgcggt attaaacatg cggatgattt
     1321 ctgctgattc gttgctgacg atggtgtggt tctttttgtc ccacagcacg ggaacagtaa
     1381 ctcgtccgct gtagtgtgga tcggcgtgga gataaagctg atacaaaaat tcatgttgat
     1441 agagcgtgtc tccggttgct cccggaaaac tgtcatcaaa ggtccagccg ttttccagca
     1501 tcagcgggtt cactacggaa acggaaataa acggttccag tcctttgagt ttgcgcatga
     1561 tcagcgtgcg gtgcgcccac gggcaggcga gtgaaacata gagatgataa cgatctttct
     1621 ctgcgataaa accgcctgtg ccagtggggc caggagcgcc atcggcagtg agccagttac
     1681 gaaatgcgga agctgaacgt tgaaatttac cgccggtaga tttggtatcg taccaggtgt
     1741 catgccagac gccgtcaatc agttgaccca ttgttctctc ctccaatagc aaaaacgagg
     1801 agcttcctcc tcgtttttta tcgttaagta tagcgcgtgc ttaccacttt ttattcagca
     1861 ggcggtcgat gctatacgcg cccggaccgg taattgccag cagcaggaat ccgccagaaa
     1921 ttgtcaggtt tttcatgaac atcagcgagt tgacgccttc agcaaagttg ctgtgaaata
     1981 aaaatgccgt cagcagcgta aagcccgcag taaacagggc tgtggtgcga gtcaggaaac
     2041 cgaacaggat tgccagacca ccaccaaact caagcagaat caccagcggc agcataaaac
     2101 ccgggacgcc catcgcttcc atatattgtt gggtacccgc gtaaccagta atttttcccc
     2161 agcctgcggt aataaacaga atcggcatta aaatgcgcgc taccagtaca ccaacatctt
     2221 ctaatttttt catcatactc tccactgcgc cactcaggct gctgattgtt tttttgtgca
     2281 agggcgcggt attagcgtcg ttgctgtcga tggagagaat cataaacgtg gtgaatgatg
     2341 attgttagca aggaaaactg tcaaaaatct tcaaaaaatt tgatgggtaa ggccggaatg
     2401 gctccggcca gagggaagtt aaccgcgaag ctgttgctgc ttgagggtcg ttttaaccag
     2461 acgccaggcg ctccatacgc caaaaccgcg tctggcccag cggaccagca tattaggatg
     2521 gcgaatcgtc cagatcgcca tcacgctact gccaaccagc gcccaggagc gcagacttag
     2581 cagcatattc cagcgacgat cgtaagcgcc tgttgcctcc agccattcac gacgactggc
     2641 agaaagatcc agccgttgtt gctggatctg gctaagcagt tgcgccttac gtcgttcacg
     2701 ttcgacttta ctgctcacgg gactcctcct cgagcagctg ccgatcgttt gccagctcat
     2761 ggcgtgtatg gcgcagcaag gtggatttac gcgatttacg taacgtccag ataccgccaa
     2821 tcagtgccag tagcagcaac accacggtgg tggcaatcat cgcattcagg cgatattgcg
     2881 gatcaaccgc ccaaataatt agcaccatca ggctcataag accaaatgca gcgaaaagca
     2941 tcgtcaggcc cagcatcagt aaaagttgaa agagattagc tttttcctct tccagctcaa
     3001 ccaccgccag ccgcagacgt gtttccacca tttcaaccat gatggaaaca attcgctgcc
     3061 cgatgcccag aacgctttta ccgggccctt gcgcgtgatg agtgtccgcc ataattaacg
     3121 acgcgacagc agaacgccga gcactacacc gattgcagcg ccaatgccca cgcccgtcca
     3181 tggattttca cgcacatact catcggcacg cgccgccgcg acacgggttt gtttggcgat
     3241 ggcatcaccg gtttcaccca ggcgataacg gctctgtttc agtgcctgct ccgctttgct
     3301 acgaatctta ctcaactctt ctttcgactt ctcgccagat gagctaagca cctcttccag
     3361 cgtatcggaa agggatttca actcagcacg cagatgttcc gtagtgtgtt ctttcgacat
     3421 agttttctcc aggtgagtaa gtagtgacta ttgtgagtta gtagtcgcgc gcttccagct
     3481 ttttcagctc ttcctgcgct tctgccagtt tccgttcgcg tttggcaatc ttatcggcat
     3541 cgcctttttg cttcgcctcg gctaaatctt gctggcgttc cgccacctca tctttctgct
     3601 ttgcgatttt cttctgatga tcggcacgca gctggctatc tgaacagttg gcccggactt
     3661 cactcagggc tttattcaga ccgtcaatac gattctggtt ttggtgtttt tcggcatagc
     3721 tgatctcctt aaggatattt tgctcctttt cctgacacag ggtagtggca taactaccgg
     3781 cactaagagc aaaaagagaa acagctaaag cgatgcggta tttcattctt gaaccttcca
     3841 ttgtgattcg gacctattac aaaggaacga ttgtccagac aacgaagcgg tagtgttaac
     3901 caccgcctca ttaatcatag acagttacgg tgaaatcacc aaagtgagtg atctgtttcg
     3961 gacaacagga agttatccga agcgatgaga gttatcccgt agccgggtca gccactgcat
     4021 agcctgactg ttattgtcct gctgcgcaat gatgtagcct tggggcaatg ttctgtcgag
     4081 gaccgctttt gcagcggcgc tctggtcgct ggagtcaaag gtaattaaca gagtgtcgtt
     4141 tttgggggtg atacttttga aaggaatgcc atgagcgtca aggtgatgcc agattgaaaa
     4201 accgtctggc atcgttgtgc cttgatgaac ggcacgaatc gccagcgtag actcttgctg
     4261 gcgaaccgcg gaccaggcca gcaacagcgt gccgaccaga agtaaaacag cgccggacca
     4321 ggctagctgg cgaagcgaca tgcgaggtat ttgcatccct tacccccgat ttccatattt
     4381 ctttttccat aatacgacca gagaacctgc caggccaaac accagcagca ccaccgggag
     4441 cagcatcagg catgacatca gctggtcctc gtactttaaa aataccggcg ttttgccgag
     4501 catgtaaccc agagttgtca ggatcaatac ccacagcaga ccgctcatcc agttgaaaaa
     4561 ctgaaagcgc gcgttattca gccctgataa cccggcaatc gtcggcagca gtgttctgac
     4621 aaacgcaata aagcgaccaa ttaacagcgc cgataaaccg tgtttatgaa aaagatggtg
     4681 tgcgcgttga tgataatgcg cgggtaaatg agatagccag ttttgtacgg tgcgggtatt
     4741 gcccagccat cgcccctgaa tatagctgac ccagcagccg aggctggcgg caacggtcag
     4801 cagcagaatc gtttgcggat agcccatcgc gcctttcgca atcaacacgc cgaccaatac
     4861 cagtaaactg tcgcccggta aaaaggccgc cggaagcaag ccgttttcaa ggaacaaaat
     4921 tacaaacaag acaaaataca acatgccaat catcgatgga ttggccaggg tttcaaaatc
     4981 ctgcgcccac agggcttgca gcaattgggt caaaagttcc attcattatt cctggtgaat
     5041 cggttaacgc cacgctggtt ttaaaagatg cactaccgca atgttattgc tgttttggta
     5101 tgttctgcgg gtttgataca tcaacacagc ctgaactatt ccctgttaaa tcgtgagata
     5161 agaaagcatt caaaaacata aattcaaatt gaatctaatt gtaacaaagg tcaacgttgt
     5221 tcgttatcaa aattacatgt ccgtggagtc tgattttgct gaatgcaggg gagggtggcg
     5281 agaggattta cacaggctga cactttatac ttttcgctta ccgacgagac aaacaggcgc
     5341 gtcgcagcgc ctgtcggaca ggtatttcat ttactgccac tggtggccgt atcgagatgc
     5401 actaccgggt tttcggcgaa cagatagcgg tcggcattga actcgaagtc gtcgctggtt
     5461 tcgttgaata acatgatctt ggtgttttcc aggtgctgcc acattgccag cttagcagca
     5521 tgaggatctt tgcgaatcag cgccttgagg atttgatcgt ggtcatcaca ccagttatcg
     5581 acggtacggg aatcaatgtg ttcgtgcagt tttttccagt acgggttatg gctacgctgg
     5641 gtccacattt tttcaacgat agccgccagg gcggagttct gcgtcgccag ggctacctga
     5701 atatggaact gcaaatccca ctcggaatca cggaagcatt gttcgccgcg cgcctgctcc
     5761 tggatggcca tcaatttcat gatgtcctgt ttcgttacct gagttgctgc gaactcggca
     5821 atattacttt cgatgagttg gcgagcctgg agtaactcaa acggaccgta attggcgaat
     5881 tccatattat tgtccgcagc ctgctgatgg cgcggctggt tggaaaccac gtgaatacct
     5941 gaacctttac gcacttcgac atagccttca acttccagca tgatgatggc ttcacgaact
     6001 accgtacggc tgacgttctt ttcatcggca ataaagcgct cagcaggcag tttatcaccc
     6061 accagataga cgccctgttc gatgcgctct ttcaggtcag cggcaagttg ttgatacaaa
     6121 cggcgtggtt cagtgatttc catatgcgct ccaggcatga tacggcagac tatatttgtt
     6181 ataccacttt tgcgagttta gctctactcg cagtgatgaa aaagccgcct gagaaggcgg
     6241 ctttgaagcg aaactaaaac ttacacgaag cgttaatgtt gcggtgcggg atcgttatgt
     6301 gtttcctgcg ccacctcaat cgccggtttg ttctgcaata cggtccagat aaccagcgca
     6361 cccagcaggt caaatactgc cagaactgcg aacagcgggc tgaagccgat ggtgtcagcc
     6421 agtgcaccga caaccagcgc aaacagcgta cttgccagcc atgcggacat cccggttaaa
     6481 ccgttcgccg ttgccacttc gttacgacca aacacgtcgg aagagagcgt aatcagcgcg
     6541 ccagacagtg cctggtgggc aaaaccaccg atacacagca gcataattgc gacatacggg
     6601 ttggtgaaca ggccgatcat acccgggcca atcatcagca ctgcacccag cgttacgacc
     6661 atcttacggg aaacgatcag gttcacacca aaccaacgct ggaacagcgg cggcaggtaa
     6721 ccaccgagga tacaaccgag gtcagcaaac agcatcggca tccaggcgaa catcgcgatc
     6781 tctttcaggt taaagccgta aactttaaac atgaacagcg ggatccacgc gttaaaagta
     6841 ccccaggccg gttctgccag gaaacgcggc agcgcgatac cccagaactg acggttacgc
     6901 aggatctgac caacggacat tttcttcgcc gtactcacct ggtgctgggc ttcctgacca
     6961 ttaataatat agtcgcgttc ttcatcggtc agatgcttct ggtcgcgcgg atgtttatag
     7021 aaaatcagcc atgccatcgc ccagataaag ctcaatgcac cggagatgat aaatgccatc
     7081 tgccagctgt gcatcacgat tgcccatacc accagcggcg gcgcaatcat cgcaccaatc
     7141 gaagaaccta cgttaaaata gcctactgcg atggaacgct ctttcgccgg gaaccattcg
     7201 gagctggctt tcagacccgc cgggatcatc gctgcttccg cggcaccgac cgcaccacga
     7261 gcaacagcca ggccacccca gctacctgcc agcgcggttg caccacagaa cacggcccac
     7321 agcacagcaa acattgcata accgattttc gtacccagca catccagtac ataacctgct
     7381 accggttgca tgaccgtata agcagcagaa taggctgcga tgatatagga atactgttgg
     7441 gtggagatgt ttaactcttc catcagagtt ggcgcagctg ccgccacagt gttacgcgtc
     7501 aggtaaccaa gcacggtgcc gagcgtcacc agtgcgatca tataccaacg taacccttta
     7561 attttacgca tgtaaaacct catcgttttg ttatatccgc aggaagcgcg gccatcatcc
     7621 cgccagaggc tgaagatgtt agttacgacc gggtaaccga aaaaaaaccg gcacgccccg
     7681 aacgttgcca tgaaagtttc gtgctaattc ggcttccgta ccggttaacc cgattcatct
     7741 aacaaatgaa tgaatcggca atttgcattc cgtcgactta tgatttgcga cggcagaaag
     7801 ataacttgtc atacaacttt aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg
     7861 gacattacct gacgacagcg aggccagtac tggcgcggcc tgcagagaga tttaccactt
     7921 tgagagtaat ttttttaact acgtttattg atctaactca cgaaaatatc ttcggactct
     7981 ggaaattggt gtgataactt tgtcagcatc gcaccataag caagctagct cactcgttga
     8041 gaggaagacg aaaatgactc cgtttatgac tgaagatttc ctgttagata ccgaatttgc
     8101 ccgccgtctg tatcacgact acgcaaaaga ccagccgatt ttcgattacc attgccattt
     8161 gccgccgcag cagattgcgg aagactatcg ttttaaaaac ctgtatgaca tctggctgaa
     8221 aggcgatcac tacaaatggc gcgctatgcg taccaacggt gtggccgagc gtctgtgtac
     8281 cggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg gcgactgttc cgcacactat
     8341 cggcaacccg ttataccact ggacgcacct cgaactgcgt cgtccgtttg gtatcactgg
     8401 caaattgctt tctccgtcaa ctgccgatga aatctggaac gaatgtaacg aactgctggc
     8461 gcaggataac ttctccgcgc gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac
     8521 caccgatgac ccgatcgatt ctctggagca tcacgcagag atcgccaaag acggctcttt
     8581 caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc ttcaacatcg aacaggcgac
     8641 ctttaacgac tacatggcga agctgggcga agtttccgat accgacattc gccgctttgc
     8701 tgacctgcaa actgccttga ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt
     8761 gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg aacgaagcgg aattggacag
     8821 cattctggcg cgccgtctgg ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa
     8881 aactgcggta ctggtgttcc ttggtgccga atatgcacgt cgcggctggg tacagcagta
     8941 ccacattggc gcgctgcgta ataacaacct gcgtcagttc aaactgctgg ggccggatgt
     9001 aggctttgac tccatcaacg accgtccgat ggcagaagag ttgtctaagc tgctgagcaa
     9061 gcagaacgaa gaaaacctgc tgccgaaaac cattctgtac tgcctgaacc cgcgcgataa
     9121 cgaagtgctg ggcaccatga gcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca
     9181 gttcggttcc ggctggtggt ttaacgatca gaaagacggt atggaacgtc agatgaccca
     9241 actggcgcag ctcggtttgc tgagccgctt tgtcggtatg ctgactgaca gccgtagctt
     9301 cctgtcatac acccgtcacg aatacttccg ccgcattctg tgccagatga tcggtcgctg
     9361 ggtggaagcg ggcgaagcac cggcggacat caacctgctg ggcgagatgg tgaaaaatat
     9421 ttgctttaac aatgcgcgtg actacttcgc cattgaactg aactaaggtc tggggttgat
     9481 atgcaataca tcaagatcca tgcgctggat aacgtcgcgg tcgctttagc ggatttggct
     9541 gaaggcacag aagtcagtgt cgataaccaa actgttaggc tgcgccagga tgttgctcgt
     9601 ggacataaat ttgcgttaac gaatatcgca aaaggggcca acgtcatcaa atatggcctg
     9661 ccgattggtt atgcattggc ggatattgcg gcgggggaac acgttcacgc ccacaatacg
     9721 cgcacgaatc tgagcgatct ggatcagtat cgctatcaac ctgattttca ggatctgcct
     9781 gcgcaagcgg cagatcgtga agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg
     9841 cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca acggcatcgc gcggcagatc
     9901 cagaaccgtt ttctgaaaga gaccaacaac gctgaaggta ccgacggcgt gttcctcttc
     9961 agccacacct acggctgctc acagctgggc gacgatcaca ttaatacccg caccatgctg
    10021 caaaacatgg tgcgccaccc gaacgcaggc gcagtgctgg tgattggtct gggctgtgaa
    10081 aacaaccagg ttgccgcatt ccgtgaaacg ctgggcgata tcgatcctga acgcgttcat
    10141 ttcatgatct gccaacagca ggatgatgag atcgaagccg gaatcgagca tttgcatcag
    10201 ctgtataacg tgatgcgcaa cgataaacgc gagccaggca aactcagcga actgaagttt
    10261 ggtctggagt gcggtggttc tgacggtctt tctggtatta ctgctaaccc gatgctgggg
    10321 cgtttctctg actacgtgat tgctaacggc ggtactaccg tactgaccga agtgccggag
    10381 atgtttggcg cagagcagtt gctgatggac cattgccgcg acgaagcaac gtttgaaaaa
    10441 ctggtcacca tggtcaacga cttcaaacag tactttattg cccatgacca gccgatctat
    10501 gaaaacccat cgccggggaa caaagcgggc ggtatcacca cgctggaaga taaatccctt
    10561 ggctgtaccc agaaagcggg ttccagcgtc gtggttgacg tgctgcgtta cggcgagcgt
    10621 ctgaaaacgc cggggctgaa cttgttaagt gcgccgggta acgatgccgt agcgaccagc
    10681 gccctggcgg gtgcgggctg ccatatggtg ctgttcagta ctggtcgtgg cacgccatac
    10741 ggcggctttg taccgacggt gaaaatcgct accaacagtg aactggcggc gaagaaaaaa
    10801 cactggatcg actttgacgc gggtcagctg atccacggta aagcgatgcc gcagttgctg
    10861 gaagaattta tcgataccat cgttgagttt gccaacggta aacaaacctg caacgagcgt
    10921 aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag
    10981 ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt ttgcttttcc atacctgata
    11041 aggatttatc atgaccgcgt attggctggc ccagggcgtg ggtgtcatcg cctttctgat
    11101 tggtatcaca acatttttca atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta
    11161 tagcgccgtt attggcgtac atttttttct tctgggcacc tatcccgctg gtgccagtgc
    11221 catccttaat gccattcgta cattgattac tttacgcacg cgcagcttat gggtaatggc
    11281 gatttttatt gtgctgactg gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact
    11341 attgccggtt atcggtacga ttgtcagtac ctgggcgctg ttccgctgta aagggctcac
    11401 catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg attcacaact tctgggcggg
    11461 gtcgataggc ggcacgatga ttgagggtag ttttctgctt atgaatggcc tgaatatcat
    11521 tcgtttctgg cggatgcaga aaaggggaat tgatccgttt aaagtagaga aaacccctcc
    11581 cgccatagac gaaaggggtt aaacaattaa ttacgcaggg cgctatttgc cagacgatcg
    11641 tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt
    11701 tcgcaagagt cctgcaatac gccgataata aagccgacgg caaccacctg catggcgata
    11761 tcgttcgaaa taccgaacat attacaggcc agtgggatca gcagcaaaga accccccgcc
    11821 acgccggatg cgccacaggc acacagagaa gccactacgc tcaacagcag cgctgtgggc
    11881 agatcgaccg gaatacccag cgtattaacc gcagccagcg tcaacacggt aatggtgatt
    11941 gctgcgcccg ccatattgat ggtggctccc agcggaatag aaacggaata ggtatcgcga
    12001 tccagattca gcttttcaca cagtgccata ttcaccggaa tgttcgccgc agagctgcgg
    12061 gtgaagaagg catacacgcc gctttcgcgc aggcacagca gcaccagcgg gaacgggtta
    12121 cgacgaattt tccaccacac cagcaatggg ttaaccacca gcgccaccag taacatacag
    12181 ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc
    12241 agggtagaag aaaccagacc aaaaatacca atcggtgcga agcgaatgac cagtttcacc
    12301 ataaaggtga cggcattcga catatcgttg accaggtttt tggtggtctc gttaccgtga
    12361 cgcagtgcga aaccgaggcc aattgcccac accaggatcc cgatgtagtt acctttcagc
    12421 agcgcgtcga tggggttgga aaccatgctc attaccagcc cgcgcatcac ttctacaatg
    12481 cctgacggtg gcgaaatatc acccgcgcta ctggataagt gcagggtaga agggaaggcg
    12541 aagctgaaaa cgactgcggc cagagcagca gagaaggtac ccagcagata gaggaacaaa
    12601 atagggcgga tattggtttt ctgcccgtgc tggtggttag caatagatgc catcaccagc
    12661 atcaacacca ggatgggggc aacggctttc agtgcgccga cgaacaaagt acctaacaga
    12721 ccaacagctt ccgccgcggg ttttgagatc catgccagaa gaatccccag aacaaggccg
    12781 accaggattt gttttaccag gctgccatga gccagacgcc ggaatagccc cggtgaacgt
    12841 tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac atccctggtg cgttctgtcg
    12901 acgcatcttt caggatgtaa caaagtgttt gcaaggtcga gtataaggaa agttacagct
    12961 tcgggaagcg aaaaatgctg gattttaaag agaggtcata ttttgcaact taatggttac
    13021 atttatttga cataaaaata aagccaacgt gcggcttcca gactgaggac tacgtcaaaa
    13081 aaggactcgg tcagtcccct cgccccgttt ggggagaggg ttagggtgag gggaacaggc
    13141 cagcatcggt gcgaaaaatt accctcaccc cgaccctctc cctggaaggg agagggagaa
    13201 aacggtgctc atgttgatga cagggtacac ggcttaggca gattaaaaat catccaacct
    13261 gctgcttatc atgccgataa ttcacccagg cgttgataat aaacgtcatc accaggatgc
    13321 caaacaccac gcccagcgag acggcgattg gaatatggta gaagtcgaca atcagcatct
    13381 tgataccgat aaacaccaga atcaccgcca ggccatattt gagcatcgag aaacgctctg
    13441 ctacgcccgc cagcaggaaa tacatcgcac gcaggccgag gatcgcaaac aggtttgagg
    13501 tcagcacaat gaacgggtca gtggtcacgg cgaagatagc cggaatgcta tccacggcaa
    13561 aaatcacgtc gctcaattcc accagaatca gtaccagcat cagcggtgtg gcgtacagca
    13621 atccattctt acgcacaaag aaatgctcgt tgtcgatggt gtcggtcatg cgcaaatgac
    13681 cgcgtagcca gcgcaccagc ggcttgtcgc caatgcctga ttcatcttca tgggcgagcg
    13741 ccatcttcac gccggtaaac agcaggaagg caccgaagat atacaggatc cagtcgaact
    13801 gcgaaatcag ccagctgcca gtgaagatca tgatggtacg cagaacaatc gccccgagca
    13861 cgccatacac cagcacgcgg cgttgtaatg ccgccggaac ggagaaatag ctgaacaaca
    13921 tcagccagac aaagacgtta tcgaccgcca gcgacttctc aatcagataa cccgtgagga
    13981 aggccagcgc ctgtggatcg gcaacggcgc gaccttcggt ttgtaccaga taccaccaga
    14041 aagcagcgtt aaacagtaac gacagcgtca cccagaccag cgaccaggcc gcagcctgtt
    14101 tcatggtcat ggcatgtgcc ccacgacgtc cctgcaacaa caggtcgata gccagcataa
    14161 tggcgacaac aacagcgaat ccgccccata gcaacggcgt gccgacagta ttcatagaag
    14221 ttccttacac ataaaaaaaa cggccaacgt cggaagacgt cagccgctgc tttttatgca
    14281 tagacctcgc cttccggcaa ggtctcactt acaacaaaaa aggaatacgt ctgcgtattc
    14341 ctttcgggtt gcccggtgac cggatgtggt ttttcacaca tcgtaatgac gatcgaccgg
    14401 caatgaagtt actccccttt gcaggtaaca aagtatgaca gaccattcag tcagtcaatg
    14461 gtctgttaca taccttttac actgttttac tttggcaatt atagttttac gctatctgcc
    14521 ggaaaaatca ccccagtctg gcggcggatc tcggtcagca gtttggctgt gatgcgactg
    14581 accgccagcc ctggatgatc caccagatgc tcatccacca gggtagcgaa cagctctgct
    14641 tcgtagagca tggtattaat atgctgcggc tgggtgagat cctgcatctg gctgccacgc
    14701 ggcacgaagc acactttctg gcattcagac agtttttcaa tcaccagcga tcccgcttcg
    14761 ccctgaatct cgcttgccag gacagaatcg ctgacctttg agtgctgcaa ggtgacgctg
    14821 aaatcaccgt aatccatcac caccacgccg tgggcatcta caccacttgc cagtaaactg
    14881 gcggttgcct gcacgctttt cggctcacca aataacgcta ccgccgacgc caggcagtaa
    14941 aagccgatat ccataattga accgttagag aatgccggat taaaggtgtt gggattctca
    15001 ccgtccaggt aacgttgata ccgcgaggaa tactggcaat agttgaaaaa aactttccgc
    15061 agtttgccga ctttcggcag cgcctggcgt aacagatgaa agttaggcag gctggccgtt
    15121 ttaaatgcct caaacagcac cacctgattt tcacgcgcgc aggcaatggc tgcatccact
    15181 tccgccagat tcgacgccag tggtttctca caaatcacat gaattttgtg gctcaggaaa
    15241 agttgcgtct gggaaaaatg cagggcgttc gggctggcaa tatacaccgc gtcgatagcg
    15301 tcactttccg ccattgcttc cagcgaagta aacagatgct cgacagaaaa ctcattggcg
    15361 aagtgctggg cctgttcaag gctgcgggaa taaacggcgg ttaacttata tttaccgctt
    15421 tcatgtgcgg cctccacgaa ctggtgagtt atccagttcg taccaatcac ggcgaaacgt
    15481 atcataacgc gtggatactc cataaaggga ttctgtcgtc aatgtagcac gcctttttca
    15541 tcttcgacgt gcgcggatac gcattatttc tgcgccggac aaccatgctc actaaacgga
    15601 ccaataatga ccgacggccc gagatatttt ggctgacgat tgaagatttt cgcgtcaatc
    15661 accgcgctga ctctggcgag tttctctcgc agacggacaa tctttccgcg tcctttttcc
    15721 aggtctggtg cgttaaaccc gatagcatcg atgccgtatt gttttgccag ccagatggcg
    15781 cgttggttat gaaattcctg cgagataatg gtgatatggt tctccccaaa cactttattg
    15841 gcacgcacca ctgaatcaag cgttgagaat ccggcatagt cacagaagat cacttttgcc
    15901 ggtacgccct ttgcgatcaa agcctgctgc atgcctgacg cttcatcgta atttttacgt
    15961 ccgttatcgc cactgaccag cagccatttc acttttccgg cgtggtacaa ctctgctgcg
    16021 gtatcaatac gtcgggtaaa gtagcgatta cccggcctgg cccccagcaa taagcccacg
    16081 ttacgcgccg gaacgacgtt gacgtcgctc caggtcagct gtttgctggc gttgaccatt
    16141 acgcgatcgg cgataaaaat ggtcgctcct gccacaagta acagtaagca ggcaatcttt
    16201 agagtccggc gagaaaaaca aatacggaga agaaggcggg caaatgcgcg cagcatggtc
    16261 gttatcctta acgaaagggg ggaggtcgat tttaggcgct aaagaaagaa aagataacgc
    16321 ctgacatcat tttcagattt tgtcctgccc aagcgacaac tgggttagcc acagtcgggt
    16381 gtcgaactca agctggtggt actggggttc catatgacag cacaactggt aaaacgcttt
    16441 gttatgctct ttctccttca ggtgcgccag ctcgtgcacc acgatcatgc gcaaaaacgg
    16501 ttccggcgcg ttgcgaaaca cggtagcgac gcggatctct gcttttgctt tcagcttgcc
    16561 gccctgcaca cgagaaacag cggtatgtaa cccgagcgcg ttttttagca catggatctt
    16621 attgtcatac atcactttat tgatcggcgg ggcattacgc agaaactgat ttttcagatc
    16681 ctgagtatat tgccagaggg ctttatcggt ggcgtagtcg tgtgttcccg gatagcgttt
    16741 tgccagcaca tcgcccagac gctgttcgtt aatcagtgtt cgcacctggg aaagtagctg
    16801 ctcgggataa ccctgaagat aagtaagatt gctcataacc gcccacgaaa aagagaaaaa
    16861 gggtatactc acgcaccctt ttcaggggca atgtcgaaat tttatcattc aggagggccg
    16921 atgagccact tagacaacgg tttccgttca ctgacactac aacgttttcc ggcgacggat
    16981 gacgttaacc cgctacaggc gtgggaagcg gcggatgaat atttgctgca acagttggac
    17041 gacacagaaa tccgcggccc ggtgttgatc ctgaatgatg cctttggtgc gttaagctgc
    17101 gcgctggcgg aacataagcc gtacagcatt ggcgactcat acatcagtga actggcgacg
    17161 cgcgagaatt tacgcctcaa cgggattgat gagtcgagcg tgaagtttct cgacagcacc
    17221 gccgactacc cgcaacagcc gggtgtggtg ctgatcaaag tgccgaaaac actggcattg
    17281 ctggaacagc aactgcgtgc gctgcgcaaa gtggtcacgc cacaaacacg tattattgcc
    17341 ggtgccaaag cccgtgacat ccacacttcc acgctggaac tattcgaaaa agtgctcggc
    17401 ccgaccacca ccacgctggc atggaagaaa gcgcgcctga ttaactgcac tttcaatgaa
    17461 ccaccgctgg ccgatgcgcc gcagaccgtt agctggaagc tggaaggtac tgactggact
    17521 atccacaacc atgcgaatgt cttctcccgc accgggctgg atatcggcgc gcgcttcttt
    17581 atgcaacatc tgccagagaa tctcgaaggg gagattgtcg atctcggttg cggtaatggc
    17641 gttattggtc tgacgctgct tgataaaaac ccgcaggcga aagtggtgtt tgtcgatgaa
    17701 tcgccgatgg cggttgcttc cagccgtttg aacgttgaaa ccaacatgcc agaggcgttg
    17761 gatcgctgcg aatttatgat caacaacgcg ctctccggcg tggagccttt ccgctttaat
    17821 gctgtgctct gcaacccgcc gtttcatcag caacatgcgc tgaccgataa cgtcgcctgg
    17881 gagatgttcc accacgcccg ccgctgcctg aaaatcaacg gcgagctgta tatcgttgcc
    17941 aaccgtcacc tggattactt ccataaactg aagaaaattt tcggcaactg caccaccatc
    18001 gccacgaata ataaatttgt ggtgctgaaa gcagtgaagc tggggcgtcg tcggtaagat
    18061 tgacagctac ctgccagata aggcgtaaac gccttatctg gcatacgttt agcacattac
    18121 ccccaggtcg gataagatgc ggcaagcgtc gcatccggca atgtctgccc agttctggaa
    18181 tcgcgctcca taaaacaaaa aatccctgtc accatcctct acattctctt gtttagcgtt
    18241 gtttaacgtt tattcttccg tcacacagat aaattcatcc gttgccaatc tgtcaacgga
    18301 tgttatcata ttgccaattt gtcaacggac gtgatgaaga atgcacctga taactcaaaa
    18361 agcattgaaa gatgctgcgg aaaaataccc gcaacataaa acggagttgg tggctctagg
    18421 gaacacgatt gctaagggat atttcaaaaa acctgagtca ttaaaagcag tattcccatc
    18481 tctggataac ttcaaatatc tggataagca ttatgttttc aatgttgggg gcaatgaatt
    18541 acgtgttgta gcaatggtct tttttgaatc gcaaaagtgc tacatacgtg aagttatgac
    18601 gcataaagaa tacgattttt ttaccgctgt tcatcgtact aaggggaaaa aatgattgcg
    18661 attgccgaca tcttgcatgc aggagaaaag ctaactgctg tggcaccttt tctggcgggt
    18721 attcagaacg aggaacaata catccaggcg ctggaactgg tagatcatct gctgctcaac
    18781 gatcctgaaa accccttact ggatctggtg tgtgccaaaa taaccgcgtg ggaagaatca
    18841 gcgcccgaat ttgcggaatt taatgccatg gctcaagcca tgcctggcgg tatagccgtg
    18901 attcgtaccc ttatggatca atatggttta accctttccg atctgccgga aattggcagt
    18961 aaatctatgg tgtcacgcgt tttgagcggg aagaggaaat taacgctgga acacgctaaa
    19021 aaattggcaa cgcgattcgg catttctccc gccttgttta ttgattaacg cgacgtgccg
    19081 gatgcgttgc atccggcact tcaatgcacg atttaaatct ccagcgccag ccgtgttccc
    19141 tgggcaattg cccgtcgtgc gtccagctcc atagccacat cgcagccgcc aattaaatgc
    19201 accgttttcc cgctatcaat cagcggctgc gccaatgcgc ggtttggctc ttgccctgcg
    19261 cagatcacca cattgtccac tgctaatacc tgcgtttcgc cgttgatcac cacatgcagc
    19321 ccgtcatcgt caatcttctg ataacttacg cctgggatca ttttcacacc ccgcgagagc
    19381 agggtggtgc gatggatcca gccggtggtt ttgcctaacc cctgtcccgg tttgctggct
    19441 ttgcgctgga gcatcacaat ctgccgtggg ctacgcggga tctgcattcc ctgcgggctt
    19501 aagccaccag cctgttgtag gctgctgtcg atcccccatt cattacagaa cccggcgata
    19561 ttctggctgg tggattcgcc cggctgactt aaatacatcg ccgtatcaaa accaatcccg
    19621 ccacaaccga tgatggcaac tttgttgccc accggcgctt tgtcgcgcag tacatcgaga
    19681 taactcaata ccttcggatg atcgatcccg tcgatgggcg gagtgcgcgg cacgatccca
    19741 ctggcgagga tcgtttcatc gaacgcctgt aactgatccg ccgtcacggt gtgattgagt
    19801 tttagcgtca cgcccgtcac ttcgatcatc cggcggtaat agcgcagcgt ttcgtaaaac
    19861 tcctctttgc cagggatctg tttggcgata ttaaactgcc cgccgatctc actgtgcgcg
    19921 tcaaacaacg ttacctgatg accacgcgcc gccgcgttaa tggcaaacgc cagcccagca
    19981 ggtcccgcac cgaccaccgc cagatttttt ttctgcatgg cgggaaggat cggcattttg
    20041 gtttcatggc aggcgcgagg attcaccaga cacgaggtga ccttgccaac gaagatctga
    20101 tcgagacacg cctggttgca gccaatgcag gtgttgatct catcggctcg ccctgattgc
    20161 gcttttgaca gcagttccgc atccgcaaga aacggtcgcg ccatcgatac catatcggca
    20221 tcgccgcgcg agagaatatc gtcggcaacc tgcggatcgt taatccggtt ggtggtcacc
    20281 agcggcagcg agacgtgacc tttcagtttg cgcgtgaccc agctaaatgc gccgcgtggc
    20341 acgggcgtgg cgatggtcgg aatacgcgct tcatgccagc caatgccggt gttgataatg
    20401 gtcgcgcccg ccgcttcaat ggcctgcgcc agctctaccg tttcggcaaa agtcccgccg
    20461 ccttcgacca ggtcgagcat cgacagtcgg tagataataa tgaagtcgtt gccaacgcgt
    20521 tcgcgtaccg cactcaccac ttcaacggca aaccgcatcc ggttgtggta atcgccgccc
    20581 cactggtcac tgcgctgatt ggtgcgcagc gtcagaaatt cgttgatcaa atacccttcg
    20641 gaacccatta cctctacacc gtcgtatcct gcctcccgtg ccagttgcgc gcagcgggcg
    20701 aaatcgtcga tcagttgcag gatctcttca tgggtgagtt catgaggaac gaaacggttg
    20761 atgggggcct gcaatgcgga cggtgcgacc agatgcggtt ggtagctgta gcgcccggta
    20821 tgcaaaattt gcagggctat tttgccgcct tcctgatgta ccgcttcggt aatggtgcga
    20881 tggtgtggga tctggctggc gtcattgagc attgcgccgc cttccatgcc aacgcctgtt
    20941 aaatctggtg caatgccgcc gctaacaatc agcgccacgc cgtgacgggc gcgttcggca
    21001 taaaacgctg ccagccgttc agcaccgtcc gggtattcct ccagcccggt gtgcattgaa
    21061 cccatcaaca cgcggttttt taacgtggta aaacctaaat ccagcggggc gaacagcgac
    21121 gggtagctca taaaattgtc cagtatgtaa aataattgtt atgtggtcgg atgagttcta
    21181 atttagccgt cgccggagaa aagggaaaag gggagtgcgg ggtttgtgat gggattcaaa
    21241 aagaatacgc ccggtggtaa cgccggatgc gacgcttgcc gggccctatc cgacctgggg
    21301 gattgcacat gtagggcgga taaggcgttc acgccgcatc cgccagtggc gcggtgcaga
    21361 cgccggatgc gacgcttgtc gggtcttatc cagcctacgg gaacgaacat gtagggcgga
    21421 taaggcgttc acgccgcatc cgccagtggc gaggtgcaga tgccggatgc gacgcttgtc
    21481 gcgtcttatc cagcctacgg gaacgaacat gtagggcgga taaggcgttc acgcagcatc
    21541 cgccggtggc gaggtgcaga tgccggatgc gacgcttgtc gcgtcttatc cagcctacgg
    21601 gaacgaatat gtagggcgga taaggcgttc acgccgcatc cgccagccgt tgcaaattat
    21661 tgcttacgga aaaaatcgtt atacaacata taaaaatgtg cggcactcca ggagaaattt
    21721 ggtgcgcctt gctgtgcgcc tgtcagcggg ttgtaatttt cctgaattgg gccatcggcg
    21781 gttaaccctt tggcgtgccg gaagaacgta tccgccagtt tcagggcatc atcgcgataa
    21841 ccgtaacgct ccatcccttt cagaccaaac cagaactgat ccacccatac gcgcccgcgc
    21901 cagtagatat cagcgccaaa ggccggattg gttaacgccg ccgttcccag cggaacaaag
    21961 gtattgaact ctttaggatc gagcatcacc ttcaccaccg cgtcggcatt ggcctgcgtt
    22021 gccgcaccgt taaacagcgg cgaccagcct tccggccctt taccgcgctc aacaatcggt
    22081 ttgcccgcgc agccgttcgc cagcggttta tcttcaatac gcacgtcata gaagtactgc
    22141 gtagccgggt cgaacataca ggtgttgatg tagtccgcga gctgctgtgc caactggcga
    22201 tagcgcttag cctcttccgg tttaccgaga atcgtcgcca tctccgccag ataatggtta
    22261 tcgctgtaca tatagctggc ctgatccacc gactcctgca atagcgagta ccccagcaac
    22321 gttccgtcct gactgcggtt ttcggcgaat ttcactgtcc agtcgctacg tttgccgccg
    22381 cttgcgacat atttatccag ctgttctttg tcgataaacc caaagacggc ggcatcatca
    22441 cgccccgatt cccacgatgc agcaacctgt gccggaattt ccagactgtc gtactggcct
    22501 ttctccacca cgcgggcgta gttgttcagc ccagactgcg tctcttcttt gttgcctttc
    22561 ttcaccgtaa acagcatctc gccgctctca gtgttgtggg ctttgtcgcg ggtcgcgcca
    22621 tattccggca cgccgttgcc gttgtgatcg cggttacgta accaccagtc gtgataggcc
    22681 accagtttcg ggtacatctc tgccacccag gttttatcct gggtgacgtt gtacacttcc
    22741 atcaccgacc aggcggcaag gctgggtttg gtatttcgtt cgttccagtt gccaccatcg
    22801 ccaccacgct cggggctaag attccacgct atcaggtcgg ggacaaagcc cacatcctgc
    22861 ggacgcacgc tgtcgccagg ctggatctgc caggagaaga ccgcgcggat attctctttg
    22921 gcgatgtccg gattgaaatg cgccatcgca aacgcctgct tccaggtatc ccacggccag
    22981 gtctgattgc cggagaacca gcgcccggtc accgacggcg tgacggtgtt atatttcacc
    23041 gcacccccag gcgagcgcca gttaccgttg agcgtttcga tcgctttcac cgcgacgcgt
    23101 gtctgttccg gcgtcgcatc cggattggtt aaccctttct tcagatactc ttcccagcgt
    23161 tgctgcgagg cggtgagata aaacgccgga cgcgccagaa tatcgcggat ctgcatttgc
    23221 tctttgctaa cttcctgaga ggtcagcaga tgggaatagg tggtatagag cgtcgtcgaa
    23281 ccgttgatat gcgccttact ggtaaagcga ttgccgttga tttcagtctg caccggcagg
    23341 gatttatgca cctgatattc tgattcaccg gaggtcagca gatcccaggt ggcgcgcact
    23401 ttgccaaagg tgactttcag gccatcacgg gtggcgctga ttttgcgctg gtagtcaggg
    23461 tattcgccag caatggtttt atcggaaagc ggtttccctt ctttcgcttc cagtttttcc
    23521 agcagttcgc catcccacac cagatccagc ggtttattgc tgatgatttt agtttccagt
    23581 agtgacgtgc gcggcgtggc gaagcgcaga gtcatttcga cctgcacatc ttttgctgtc
    23641 agtttttgca ccagcgcgcc gggaatattg tatgcctcca gcgtgaagtt gactttcttg
    23701 ccgtcctgcc agacggtcag gcggtcgaaa ttgctggcca taaagttgat gtattcttcc
    23761 gtcagcagcg caacgcccgg aaagccgccc atggtgttcg ggccgtctgg caacagatga
    23821 ccatgccagg ctccgagatc gaaaaacgga ttaaaacgct ggtgatcgtc gtaatcgtaa
    23881 tccttcatgt actgcggcgc accagtacgg ttaatcacgt ttttataatt gtcggcgtta
    23941 gtggcatggg cggaaaaact tatcagcaga gcgcaggtta ctggcgttaa aatagttttg
    24001 attttcatga aattacagtc cttattacca gtagaaatat tgcatcagga agatttggtc
    24061 gctgggatcg ttagaggcat ttttcatgta gaagcggcta tcaaaagccg ttgcgaaacg
    24121 tccgccgcca tattcgtacc agataccaaa ctgcatgaaa gaggtggttt catcgtcagc
    24181 gtggtcaggg cgatgtttgg cgtaggtgta agcggtggag agataaacgc cctttaacgc
    24241 ttcatacatg gcgctcacca tcatcccgct ttcacttccc ggtacgtcca gcccgtcggc
    24301 acgtccggta tggtgccagg cgcgagcggc aaagttaggg gtaagcagtc ccgtgagata
    24361 gagttgtcct gtgccgtcgg taatttccag accattcatc caggtgatgc cttcggcaag
    24421 gtcatactgg atgtaaccgt tcatcattgc cggataggtg tatttgtcgt cgtagcgatc
    24481 gtacttgcca aaatgtagcc atgctttgct ttcgtcatga tgtccggcag gtgtggcggt
    24541 gacgctataa cgaaagttgc cgcttaagtt ctgcaccttc agcgcggtca taatgtcgcg
    24601 ggtattggga atgacgtagc ccagatcggg tgtgaagtcg ccccaccatt gcaggtcatc
    24661 cagcgaggaa tcctgacgca gactgagcat gatttcggtg ccatcattgg ttcgataacc
    24721 gccatagaaa cggttaatcc caccttcgaa accgccccag ctatggtcag gcacccaggc
    24781 attgccgtcg tggtcggcct gcacggtcca gccttcgccg taaatcaggc cgaaccagga
    24841 gccgtacttc atctccaggc caccttcaat ataggtgccg tcgctgtatt tatgtttatt
    24901 ctcaccctct aaatccatat atccaccaac ccctaattcg ccgtaaaaac gaaatgaagg
    24961 agaagatgtc gaggtaattg cgggggtttt tgtgtcgtca tgttctgccg gacgtgcttc
    25021 ttctcctgcc gcagaaaagg cgtagcaaaa cggcagagca agtaatgctc tgcatggtgc
    25081 agtgattaat ttcattgtgt taatcca
//
LOCUS       DBFIFM010000051        24961 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.051, whole genome shotgun sequence.
ACCESSION   DBFIFM010000051 DBFIFM010000000
VERSION     DBFIFM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.051"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..2031
                     /gene="yihQ"
                     /locus_tag="N7C74_001823"
     CDS             <1..2031
                     /gene="yihQ"
                     /locus_tag="N7C74_001823"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HGU2431704.1"
                     /translation="TPRPQLPDFEFHQNNDSFTLHFRQRLILTHSKDNPCLWIGSGIA
                     DIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGRLL
                     LELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQTY
                     VTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALWED
                     KATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMRNA
                     GVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYINP
                     YVASDKDLCEEAAKRGYLAKDVAGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKKNM
                     IELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEILF
                     FMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGYTT
                     LFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFTTL
                     KPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYSLKYQYLLGRDILVAPVHEEGRSDW
                     TLYLPEDNWVHAWTGETFHGGEITVEAPIGKPPVFYRADSEWAALFASLKNI"
     gene            2077..3462
                     /locus_tag="N7C74_001824"
     CDS             2077..3462
                     /locus_tag="N7C74_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2431705.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFIGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPANPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFSGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            3502..4905
                     /locus_tag="N7C74_001825"
     CDS             3502..4905
                     /locus_tag="N7C74_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2431706.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLSIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCI
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            4972..5664
                     /gene="ompL"
                     /locus_tag="N7C74_001826"
     CDS             4972..5664
                     /gene="ompL"
                     /locus_tag="N7C74_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HGU2431707.1"
                     /translation="MKNINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFTPWFNLTVRNRFNHNNYSSTDLNGDLDNNDSYEIGNYWNFIITDKF
                     SYTFEPHYFYNINDFNSGNGTKHHWEITNTFRYRINEHWLPYIELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(5755..7020)
                     /locus_tag="N7C74_001827"
     CDS             complement(5755..7020)
                     /locus_tag="N7C74_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2431708.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGITIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(7122..8102)
                     /locus_tag="N7C74_001828"
     CDS             complement(7122..8102)
                     /locus_tag="N7C74_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HGU2431709.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVREAYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKELLTHLICLGDEEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPEKHLLDERLRKEKEDAINQINHVRNVLQQIKQEASHLLNH"
     gene            complement(8110..8820)
                     /locus_tag="N7C74_001829"
     CDS             complement(8110..8820)
                     /locus_tag="N7C74_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU2431710.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(9037..10860)
                     /gene="typA"
                     /locus_tag="N7C74_001830"
     CDS             complement(9037..10860)
                     /gene="typA"
                     /locus_tag="N7C74_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HGU2431711.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            11233..12642
                     /gene="glnA"
                     /locus_tag="N7C74_001831"
     CDS             11233..12642
                     /gene="glnA"
                     /locus_tag="N7C74_001831"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HGU2431712.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            12816..13865
                     /gene="glnL"
                     /locus_tag="N7C74_001832"
     CDS             12816..13865
                     /gene="glnL"
                     /locus_tag="N7C74_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HGU2431713.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            13877..15286
                     /gene="glnG"
                     /locus_tag="N7C74_001833"
     CDS             13877..15286
                     /gene="glnG"
                     /locus_tag="N7C74_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HGU2431714.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            15398..15508
                     /locus_tag="N7C74_001834"
     CDS             15398..15508
                     /locus_tag="N7C74_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HGU2431715.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(15698..17071)
                     /gene="hemN"
                     /locus_tag="N7C74_001835"
     CDS             complement(15698..17071)
                     /gene="hemN"
                     /locus_tag="N7C74_001835"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HGU2431716.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(17260..17766)
                     /gene="yihI"
                     /locus_tag="N7C74_001836"
     CDS             complement(17260..17766)
                     /gene="yihI"
                     /locus_tag="N7C74_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HGU2431717.1"
                     /translation="MKPSSPSRSKGHAKARRKTREELNQEARDRKRQKKRRGHAPGSR
                     AAGGNNTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELLE
                     TDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQED
                     MMRLLRGN"
     gene            complement(17885..18051)
                     /locus_tag="N7C74_001837"
                     /pseudo
     CDS             complement(17885..18051)
                     /locus_tag="N7C74_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18348..18980
                     /gene="yihA"
                     /locus_tag="N7C74_001838"
     CDS             18348..18980
                     /gene="yihA"
                     /locus_tag="N7C74_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HGU2431718.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFNEMQPVEETQDGE"
     gene            complement(19362..22148)
                     /gene="polA"
                     /locus_tag="N7C74_001839"
     CDS             complement(19362..22148)
                     /gene="polA"
                     /locus_tag="N7C74_001839"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HGU2431719.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHRDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            22512..23444
                     /locus_tag="N7C74_001840"
     CDS             22512..23444
                     /locus_tag="N7C74_001840"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HGU2431720.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(23485..24915)
                     /locus_tag="N7C74_001841"
     CDS             complement(23485..24915)
                     /locus_tag="N7C74_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HGU2431721.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDSSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQQSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         6053 a         6282 c         6042 g         6584 t
ORIGIN      
        1 acgccacgcc cacaattacc agattttgaa tttcatcaga ataacgacag ttttacccta
       61 cattttcgac aacgtcttat tttaacccat agcaaagata atccttgttt atggattggc
      121 tcaggtatag cggacatcga tatgttccgc ggcaatttca gcattaaaga taaactacag
      181 gagaaaattg cgcttaccga cgccatcgtc agccagtcac cggatggttg gttaatccat
      241 ttcagtcgtg gttctgacat tagcgccacg ctgaatatct ctgccgacga tcagggccgt
      301 ttattgctgg aactacaaaa cgacaacctt aaccacaacc gcatctggct gcgccttgcc
      361 gctcaaccag aggaccatat ctacggctgc ggcgaacagt tttcctactt cgatctgcgc
      421 ggcaaaccgt tcccgctatg gaccagtgaa caaggcgttg gtcgcaacaa acaaacctat
      481 gtcacctggc aggccgactg caaagaaaat gcgggcggcg actattactg gactttcttc
      541 ccacagccta cgtttgtcag cacgcagaag tattattgcc atgttgataa cagttgctat
      601 atgaacttcg actttagtgc cccggaatac catgaactgg cgctgtggga agacaaagca
      661 acgctgcgtt ttgaatgtgc tgacacatac atctccctgc tggaaaaatt aaccgccctg
      721 ctgggacgcc agccagaact gcccgactgg atttatgacg gagtaacgct cggcattcag
      781 ggcgggacgg aagtgtgcca gaagaaactg gacaccatgc gtaacgcggg cgtgaaggtc
      841 aacggcatct gggcgcagga ctggtccggt attcgtatga cctcttttgg caaacgcgtg
      901 atgtggaact ggaagtggaa cagcgaaaac tacccgcaac tggattcgcg tatcaagcag
      961 tggaacaaag agggcgtgca gttccttgcc tatatcaacc cgtatgttgc cagtgataaa
     1021 gatctctgcg aagaggcggc aaagcgcggt tatctggcaa aagatgtcgc cggtggcgac
     1081 tatctggtgg agtttggcga gttctacggc ggcgttgtcg atctcaccaa tccggaagcc
     1141 tacgcctggt tcaaggaagt tatcaaaaag aacatgattg aactcggctg cggcggctgg
     1201 atggctgact tcggcgagta tctgcccacc gacacgtacc tgcataacgg catcagcgcg
     1261 gaaattatgc ataacgcctg gcccgcgctg tgggcgaaat gtaactacga agcccttgaa
     1321 gaaacgggca agctcggcga gatcctcttc tttatgcgcg ccggttctac cggtagccag
     1381 aaatactcca ccatgatgtg ggcgggtgac cagaacgtgg actggagtct ggacgatggc
     1441 ctggcgtcgg ttgtcccggc ggcgctatct ctggcaatga ccggacatgg cctgcatcac
     1501 agcgacattg gcggttatac caccctgttt gagatgaagc gcagcaaaga gctgctgctg
     1561 cgctggtgcg atttcagcgc cttcacgccg atgatgcgca cccacgaagg taaccgtcct
     1621 ggcgacaact ggcagtttga cggcgacgcg gaaaccatcg cccatttcgc ccgtatgacc
     1681 accgtcttca ccaccctgaa accttacctg aaagaggccg tcgcgctgaa tgcgaagtcc
     1741 ggcctgccgg ttatgcgccc gctgttcctg cattacgaag acgacgcgca gacctattcc
     1801 ctgaaatatc agtacctgtt aggtcgcgac attctggttg ctccggtaca cgaagaaggt
     1861 cgcagcgact ggacgctcta tctgccagag gataactggg tccacgcctg gaccggagaa
     1921 accttccacg gtggagaaat aactgttgaa gcgcccatcg gcaagccgcc ggtcttttat
     1981 cgcgccgaca gcgaatgggc tgcactgttc gcctcgttaa aaaacatcta atcatgcccg
     2041 cccgtgggga aactcacggg caaatggaga ataacgatga gtcacatcac aacggaagat
     2101 ccggcaactt tgcgcctgcc ctttaaagag aaactctctt acggtatcgg cgatctggcc
     2161 tctaacatcc tgctggatat tggtacgctt tatcttttga agttttatac cgacgttctg
     2221 gggctacctg gcacctatgg cggcattatc tttttgatct cgaaattctt taccgccttt
     2281 accgatatgg gaaccggcat catgttggat tcccggcgta agattggccc gaaaggtaag
     2341 ttccgccctt tcattttgta cgcgtcattc ccggtcacct tattggcaat cgctaacttt
     2401 atcggcacac cgttcgatgt cactggtaaa acggtgatgg ccactattct gtttatgctc
     2461 tacgggctgt ttttcagcat gatgaactgc tcctatggcg cgatggtgcc tgctattacc
     2521 aaaaacccca acgagcgcgc atcactggcg gcatggcgtc agggaggcgc tacgctgggc
     2581 ctgctgctgt gcacggtggg attcgtgccg gttatgaatc ttatcgaagg taatcagcaa
     2641 cttggctata tcttcgccgc cacgctgttt tcactgttcg gcctgctgtt tatgtggatc
     2701 tgctactcgg gcgtgaaaga gcgttatgtc gaaacccaac cagccaatcc ggcgcaaaag
     2761 cctggcctgc tgcaatcttt ccgcgcaatt gccggtaacc gcccactgtt cattctgtgc
     2821 attgccaacc tctgcacttt aggggcgttt aacgtcaagc tcgccatcca ggtctattac
     2881 acccagtacg tacttaacga tcccatcctg ttgtcatata tgggattttt cagcatgggc
     2941 tgtattttca tcggtgtgtt cctgatgcct ggcgcagtca ggcgttttgg taagaagaag
     3001 gtctatatcg gcggcctact gatttgggtg ctgggcgatc tgctcaacta tttcttcagc
     3061 ggcggttcgg tcagcttcgt ggcgttctcc tgcctggcat tcttcggctc agcgtttgtt
     3121 aacagcctga actgggcgct ggtttccgac accgtcgagt acggcgagtg gcgtaccggc
     3181 gttcgttcgg aaggaacggt ctacaccggc ttcaccttct ttcgcaaagt gtctcaggcg
     3241 ctggctggtt tcttccccgg ctggatgctg acgcaaatcg gttatgtgcc gaacgtggcg
     3301 caggctgacc acaccattga agggttgcgc cagctgatct tcatctaccc aagcgcactg
     3361 gcggtagtca ccattgtggc gatgggctgc ttctacagcc tgaacgagaa gatgtatgtc
     3421 cgcattgttg aagaaataga agcccgtaaa cgcacggcgt aataataaac aatgccctgc
     3481 ggggcgttat aaggagtgat tatgtctgac cataatccac tgacattaaa actgaatctg
     3541 cgggaaaaaa tcgcctatgg tatgggcgac gttggttcga atttaatgct tagcatcggt
     3601 actctgtatc tcctcaaatt ttataccgat gaattaggga tgccagctta ctatggcggg
     3661 attatctttc tggtggcgaa gttttttacc gcgtttaccg atatgctcac cggattttta
     3721 ctcgattcgc ggaaaaatat tgggcctaaa ggcaaatttc gtccctttat tttatacgca
     3781 gcggttccgg cggcgttaat tgcaacgctc cagtttatcg ccaccacttt tagtttgccg
     3841 gttaaaacga cgattgccac cgcgctgttt atgatgtttg gactttcata cagtctgatg
     3901 aactgctcgt atggtgcgat gatcccggcc attactaaaa acccgaatga gcgcgcgcag
     3961 ctcgcggctt accgccaggg tggtgcaacc atagggctat tgatttgtac cgtggcgttt
     4021 attccgttgc agtcgctttt ttctgactca accgtcggtt atgcctgtgc ggcactgatg
     4081 ttctccattg gtggctttat ttttatgatg ctgtgctaca gaggcgtcaa agagcattat
     4141 gtggacacag caccaaccgg acataaagcc agtattctca aatctttttg cgcgatattt
     4201 cggaatccgc cattgctggt tttatgcatt gctaacttgt gtaccctggc ggcatttaat
     4261 atcaaactgg cgattcaggt ctattacacc cagtatgtgc tgaatgatat taatttattg
     4321 tcgtggatgg gattcttcag tatgggatgc atcctcgtcg gcgtattact ggtgccatta
     4381 actgtaaaat gttttggtaa aaaacaggtt tatttagctg gcatggtgct atgggcggtt
     4441 ggtgatatcc tgaattattt ctggggaagt aactctttca ctttcgtcat gttctcttgt
     4501 atcgcctttt ttggcacggc ctttgtaaac agcctgaact gggcactggt gccagatacc
     4561 gtagattacg gtgaatggaa aaccggtatt cgtgccgaag gttctgttta taccggttat
     4621 accttctttc gtaaaatttc tgccgcactt gctggcttct tgccaggcat tatgctgacg
     4681 caaatcggtt atgttcccaa catcgcgcaa agtgatgcga cattacaggg tttgcgtcag
     4741 ctcattttta tctggccttg tgcactggca attattgctg cattgacgat gggattcttt
     4801 tacacactca atgaaaaacg gtttgcgttg attattgagg aaatcaacca acgcaaaaat
     4861 aaagagatgg cgacagaaga aaaaacggct tccgtaacgt tataaatgtc aatagccgct
     4921 atttccatcc tggtgggtgg cggcctccct acgtttaaaa atggacttat tatgaaaaat
     4981 attaatgcaa taattttatt atcttctctg acctctgcct cagtattcgc tggcgcatac
     5041 gtcgaaaaca gggaggcata taatctcgcc tccgatcagg gcgaggttat gctacgtgtg
     5101 ggttataact tcgatatggg cgcgggtatt atgttaacca atacctacac ctttcagcga
     5161 gaagatgaac taaaacatgg atataacgaa atcgaaggtt ggtatccgtt atttaaacca
     5221 accgataaat taaccatcca gcccggtggc ttaattaatg ataagagtat cggttcgggt
     5281 ggtgctgttt atctggatgt taactacaaa tttactccgt ggtttaacct gacagtgcgc
     5341 aaccgtttta accataataa ctacagctca accgatttaa acggtgatct ggataataat
     5401 gacagctacg agattggcaa ctactggaac tttattatta ccgataagtt ctcatatacc
     5461 tttgagccgc attattttta taacattaat gatttcaata gcggcaatgg cacgaagcat
     5521 cactgggaaa tcactaacac cttccgttac cgcattaacg agcactggct gccctatatt
     5581 gaattgcgtt ggttagatcg caacgtggaa ccgtaccacc gcgagcaaaa ccagattcgt
     5641 atcgggacga agtatttctt ctgatgtgaa actgccggat gcggcgtaaa cgccttatcc
     5701 ggcctacggt tgatacggtt ttcgttggcc ggattaagta catccggcca ttaattaagc
     5761 ttcagccgcc agcatctctt tcttacgttg gctaatacgc ttaaccagca ttacggaaac
     5821 caccgcacca cagaatgcga agcaggccat aatgccaaac acgattttgt agccaataat
     5881 ccccggattt aaatccagaa tgtagccata cagactgaag cagaacatcg ccggggcgta
     5941 accaataaag ctacccaacg ccatcgccgc gcctgtttta ttttcagcaa tttttgcttc
     6001 gccgataggt gcaaaaaata cggctcgctg tgtaaagact atcgcgccaa agcccagcgt
     6061 acatgccatc cctaaataga ccggcatact ttcgtgcggc agcataatca acagtacgag
     6121 cgcagcggta ctgatgataa aggtgtagca tagatattta ctcggcgatt tcaggatttt
     6181 atctgaaatc atgccaccaa tcggtccacc aatcattttc agacagtatt ggttaatgat
     6241 gccgtaagcc cccaccagcg caacgggcaa tagatagatg tttttcagga atggaatgaa
     6301 gaatgtcagg ccacagtaaa ccgcgtaaac gaagaagacg ttgaaagcga taagccagat
     6361 agttttgtct ttcagcaccg aggtcatact ggtgttttgc gatgccccgt cttctttttt
     6421 cacctcaacg gacggtgcct cttctttgtc attcaggaca aagaaaatag taatgcctac
     6481 ggcaatcaca ataagggaat agaaccagat gcctgcttta aaacctaata agccactgcc
     6541 aaaccaggta aatactgcca acgcagaaaa tgccaccacg gtatcgacaa tgccacgccc
     6601 tgtttcgaag aagccaaaca accgaccttg ttgttcgcta ttgcccaatc gacttaccga
     6661 cttgagcaag accggccagt tcatcatgtc gcaagtaacg ccaaatagcg cccagacaaa
     6721 gaggatgccc caataccccg gcattgtcgt caaataaaca cccagtaatc ctgtcgcaat
     6781 gagtgagaat gacatggtgt atctgcgcgg tagtttatcg gcaaaataaa tagacagaaa
     6841 aaagcccact gtggtaacaa atgagtttac cgacatagca ttaccaattt gaccattggt
     6901 caaatggaaa tattcctgca tcgggatata aaacgcatct ttcagcgacg gtaatttata
     6961 aattgtaccg ccacacagtg ttaatagact aaataacgcc catttctttt tcgtgagcat
     7021 aacatcccct aagcgatttt aaaaatcact gttttaaagg caataagata ccgaccattc
     7081 ttatggaatg gttttattaa ctgcgtatga gttaaaactt gttagtggtt cagaagatgg
     7141 ctcgcctctt gtttgatttg ttgtagtacg ttacgcacat gatttatctg attaattgca
     7201 tcttcttttt ctttacgtaa ccgctcatcc agcaaatgct tttctggcaa tggtgtttca
     7261 ctcatctggc gataagggat ccacggatta tcatcttcgt cttcaaagcg gaacgctgga
     7321 gcataataat caacctcttc ttccaggcca tatgccgtca cctgcggttc ttcatcgccg
     7381 aggcagataa ggtgtgttaa taactctttg aacggcatat ctccctgacc tgaaatacag
     7441 gctttatgac ccaggccacc ttgttcttta acgatcaagg catctttgat atggacctgg
     7501 gtaatatgcg gtgccatcgt ttttaaggcg tctatgggat gctcatttgc attgatcata
     7561 ttcgcaaaat caaacagtaa ggaaagagac tccatctcac tttctttgac cagcgacacc
     7621 aactcatgac tttttaaatc ttcatgctgt tcgatcgtaa aagtcaggcc gctatcctga
     7681 tacgcttccc gcacataggc aatgtcgtta gcgatgatcg ataatacgtc gcgcaaatta
     7741 ccttcataac gtggataaaa acgtacggac gatgccccag ttttcaacgc gatggcgacg
     7801 gcttcgtcaa tagatgcctt atctgaggcg ctggtttcaa tatgaatatc aaggttcaga
     7861 cggcgggctt tatccccaaa ggcagagagt tctttatcgt ccatattacc aagagaaaaa
     7921 cgctcgccat ccagaacatg gattttgacg ccacgtagat tattttcact tgcgatatca
     7981 agtaaatcag caggcaacac tctttccagc cgcatattta aatgaaaggc ataagcatgt
     8041 aaataaagag gaagagtgtc gacacgttgt agaatctttc ttgcgttatt tattgtcacc
     8101 ataaaacacc tacagattaa tgatgaggtc tacggcctca aatcgccaat attcaatatc
     8161 tatctggaca atattgcctt gctggtcggc gcgatgcttt tcaattaaga tcgctggcat
     8221 acccgcagaa ccgccaagat acttgctgat atcgcccggc attctgacgg gtttgaaaat
     8281 caattttttt actaccgtct ctttttggta gcacttttcc cagactgcag aaaatgagtg
     8341 gttttcaagt tgttcaataa aacccggagc aacttcagga ttaatatatg tttcgtgata
     8401 gaaaacttta tgtccttcca gcgcccccca gccggtgatg cgatagagtt cagttgatgg
     8461 tgtcactgca atcagtggcg cgagcgtctc gggaatatcg ctggtacggt ttttctcggt
     8521 aaacccccag gaaggctctc gcccttgctc aattgcggcc cgctgaaagc tcgccgacag
     8581 ctccggactg taattaaatc gcggctgggt cacgaaccac cccttacgat ccttacggaa
     8641 gattttggat tctgcctcga ggttcaacaa cgcctggcgc agcgtcatac gtttaatgcc
     8701 cagcagttct cccagctctc tttctgaagg gagttttcca cccggcgtgg tgatgccatc
     8761 tttcaaccac cgatccagtt tctcttttgc attttctacg gtggattgat tctccgccat
     8821 tgtttaatcc ttttggttta aaccagatga agcatcttac gcttacgcca aaaaagaaag
     8881 caattaacgg cgtatgactt tgcagataat tgtgacaaag ctctctattg acgtaaccac
     8941 agacttataa gggagatact cgcattggtg tgcgctcacg acaggcaaaa aaaaacctgc
     9001 catcgctggc aggtttttta tgactaaaaa acgaaattaa tcgtctttcg gtgcgcggtt
     9061 ggcgcgacga cgatcgtttt ccgtcaggtg acgtttacga atacggatag aggtcggagt
     9121 cacttctacc agttcgtcat catcgatgaa ctccagagct tgttccagag tcatgcggat
     9181 aggcggaacc agaacaacgg cttcgtcagt accggaagca cgcatgttgg tcagtttctt
     9241 accggtcagg cagtttacag tcaggtcgtt agagcggcta tgaataccga taatctgacc
     9301 ttcgtaaact tctgcaccgt gaccgaggaa cagcttaccg cgatcctgca aaccgaacag
     9361 cgcgaacgcg accgctttac cctgaccgtt agagatcagt acgccgttct gacgctgacc
     9421 cacttcaccc ggacgcacgt cgtcgtagtg gctgaaggtg gagtacagca gacccgtacc
     9481 ggaagtcatg gtcatgaact cagaacggaa gccaatcaga ccacggcttg ggatcacgta
     9541 gtcgagacgt acgcggcctt taccgtctgg attcatgttt ttcaggtcgc ctttacgttc
     9601 gcccagcgcc tgcattacag aaccctgatg ctgttcttca acgtccagag tcacgttttc
     9661 atacggctct tgtttacgac cgtcgatttc acggaagata actttcggac gggataccgc
     9721 cagttcgaaa ccttcacgac gcatgttttc gatcagaaca gacaggtgca gttcgccacg
     9781 accagaaacg cggaacgcat cggcgtcttc ggtttcttct acgcgcagcg caacgttgtg
     9841 taccagttct ttgttcagac gatccaggat ctgacgagac gttacgaact taccttcttt
     9901 accgcagaac ggcgaggtgt taacgcagaa gaacatagaa acggtcggct catcaacgga
     9961 gagtgccggc agcgcttcaa cgttttgcgt gtcgcaaacg gtgtcagaaa tgttcagttc
    10021 gcccaggccc gtgatcgcca cgatatcgcc agcttccgcc agatcggttt cgatacgttc
    10081 cagaccgagg tggcccagca ctttaccaac tttcgcgttg cgggttttgc cttcgctatc
    10141 gatgatagta acctgctggt tcggcttcac tttaccgcgc ttgatgcggc caatgccgat
    10201 aacgccaaca tagctgttgt aatcgagctg agaaatctgc atctggaacg gaccgtcaag
    10261 gtcaacgtcc ggcgcaggaa cgtggtcaac aatcgcctgg tacagcgggg tcatgtcttc
    10321 cgccatatct tcgtggtcca gacccgcgat accgttcagc gcagaagcgt aaacgatcgg
    10381 gaagtccagc tgctcgtcgg tcgcgtcgag gttaacgaac agatcgaata cctgatccac
    10441 aacccaatca ggacgcgcgc cagggcggtc aactttgttg ataacaacaa tcggcttcag
    10501 gccgtaagca aacgcttttt tggttacgaa gcgcgtttgc ggcatcgggc cgtcaaatgc
    10561 gtcaaccacc agcagcactg agtctaccat ggacattaca cgttcaactt caccaccgaa
    10621 gtcggcgtgc cccggggtat caacgatgtt gatacggtaa tcattccatt tgatagcggt
    10681 gtttttcgcg aggatggtaa tcccacgctc tttctccaaa tcgttggagt ccatcacgcg
    10741 ctcttgggtt tcggcacgag agtcgaacgt accggattgt tggagcagct tgtctaccag
    10801 ggtggtttta ccatggtcta cgtgcgcgat gatggcgata ttacgcaatt tttcgatcac
    10861 aactttgcct caggcattag aaatagcgcg ttattgtaca cggattaatc gcactacaaa
    10921 acaggatcac aaacatcctc cgcaaacaag tattgcagag tccctttgtg atcgctttca
    10981 cggagcataa aaagggttat ccaaaggtca ttgcaccaat atggtgctta atgtttccat
    11041 tgaagcacta tattggtgca acattcacat catggtgcag cccttttgca cgatgttaag
    11101 catgataacg ccttttaggg gcaatttaaa agttggcaca gttttcgctt tatctttttt
    11161 acggcgacac ggccaaaata attgcagatt tcgttaccac gacgacaatg accaatccag
    11221 gagagttaaa gtatgtccgc tgaacacgta ctgacgatgc tgaacgagca cgaagtgaag
    11281 tttgttgatt tgcgcttcac cgataccaaa ggtaaagaac agcacgtcac tatccctgct
    11341 catcaggtga atgctgaatt cttcgaagaa ggcaaaatgt ttgacggctc ctcgattggc
    11401 ggctggaaag gcattaacga atccgacatg gtgctgatgc cagacgcatc caccgcagtg
    11461 attgacccgt tcttcgccga ctccaccctg attatccgtt gcgacatcct tgaacctggc
    11521 accctgcaag gctatgaccg tgacccgcgc tctattgcga agcgcgccga agattacctg
    11581 cgttccactg gcattgccga caccgtactg ttcgggccag aacctgaatt cttcctgttc
    11641 gatgacatcc gtttcggatc atctatctcc ggttcccacg ttgctatcga cgatatcgaa
    11701 ggcgcatgga actcctccac ccaatacgaa ggtggtaaca aaggtcaccg tccggcagtg
    11761 aaaggcggtt acttcccggt tccgccggta gactctgctc aggatattcg ttctgaaatg
    11821 tgtctggtga tggaacagat gggtctggtg gttgaagccc atcaccacga agtagcgact
    11881 gctggtcaga acgaagtggc tacccgcttc aataccatga ccaaaaaagc tgacgaaatt
    11941 cagatctaca aatatgttgt gcacaacgta gcgcaccgct tcggtaaaac cgcgaccttt
    12001 atgccaaaac cgatgttcgg tgataacggc tccggtatgc actgccacat gtcgctgtct
    12061 aaaaacggcg ttaacctgtt cgcaggcgac aaatacgcag gtctgtctga gcaggcgctg
    12121 tactacattg gcggcgtaat caaacacgct aaagcgatta acgccctggc aaacccgacc
    12181 accaactctt ataagcgtct ggtcccgggc tacgaagctc cggtaatgct ggcttactct
    12241 gcacgcaacc gttctgcgtc tatccgtatt ccggtggttt cttctccgaa agcacgtcgt
    12301 atcgaagtac gtttcccgga tccggcggct aacccgtacc tgtgctttgc tgccctgctg
    12361 atggccggtc ttgatggcat caagaacaag atccatccgg gcgaagccat ggacaaaaac
    12421 ctgtatgacc tgccgccgga agaagcgaaa gagatcccac aggttgcagg ctctctggaa
    12481 gaagcactga acgagctgga tctggaccgc gagttcctga aagccggtgg cgtgttcacg
    12541 gatgaagcga tcgatgcgta catcgctctg cgtcgcgaag aagatgaccg cgtgcgtatg
    12601 actccgcatc cggtagagtt tgagctgtac tacagcgtct aatagttgaa gttgtactac
    12661 ccggcgcaac aacgccggga tttagttgcc gtggaaactt tcagcccatc tctgcatggg
    12721 cttttttctc cgtcaattct ctgatgcttc gcgcttttta tccgtaaaaa gctataatgc
    12781 actaaaatgg tgcaacctgt tcaggagact gctttatggc aacaggcacg cagcccgatg
    12841 ctgggcagat cctcaactcg ctgattaaca gtattttgtt aatcgatgac aacctggcga
    12901 tccattacgc caaccccgcc gcgcaacaac tgctcgccca aagctcccgc aaattgtttg
    12961 gtacgccgtt accggaactg ttgagctact tctcattaaa tattgagctg atgcaagaaa
    13021 gtctggaggc ggggcaaggt tttaccgata acgaagtgac gctggtcatc gacggtcgct
    13081 cgcatatcct ttctgtgacg gcccagcgta tgccggacgg catgatcctg ctggagatgg
    13141 cgccgatgga taaccagcgc cgcttaagcc aggaacagct acaacatgct caacaagttg
    13201 ctgcccgtga tttagtgcgt ggcctggcac atgagattaa aaatccgctt ggcggtttac
    13261 gtggcgcggc gcagctactc agcaaagcat tacctgaccc gtcactgctg gaatatacca
    13321 aagtgattat cgaacaggcg gaccggctgc gaaatctggt cgaccgtctg ttggggccgc
    13381 agctgcccgg tacgcgcgtt accgaaagta ttcacaaagt agctgaacgc gtggtaacgc
    13441 tggtgtcgat ggaactgccg gacaacgtgc ggttgattcg tgattacgat cccagcctgc
    13501 cggaactggc gcacgacccg gatcaaattg aacaggtctt gctgaatatt gtgcgcaatg
    13561 cgctacaggc gctggggccg gaaggcggtg aaatcattct gcgtacccgc accgcgtttc
    13621 aactgacctt acacggcgaa cgctatcggc tggcggcgcg gattgatgtg gaagataacg
    13681 ggccaggcat tccgcctcat ttgcaggata cgctgtttta cccgatggtc agcggccgcg
    13741 aaggtggcac cgggcttggc ttatccatcg ctcgtaattt gattgatcag cattcaggca
    13801 aaattgaatt taccagttgg ccagggcata ccgagttctc ggtttacctg cctatcagga
    13861 aataaaggtg acgtttatgc aacgagggat agtctgggta gtcgatgacg atagttccat
    13921 ccgttgggtg cttgaacgtg cgctcgctgg agcgggttta acctgtacaa catttgagaa
    13981 cggcgcggag gtactggagg cgctggcgag caaaacgccg gatgtgctgc tttcagatat
    14041 ccgtatgccg ggaatggacg ggctggcact gctcaagcag attaaacagc gccatccgat
    14101 gcttccggtc atcattatga ccgcacattc cgatctggat gctgccgtca gcgcctatca
    14161 acaaggggcg tttgattatc tgcccaaacc gtttgatatc gacgaagccg tggcgctggt
    14221 tgagcgcgcc atcagtcatt accaggaaca gcagcagccg cgtaatgttc agcttaacgg
    14281 cccaacgacc gatatcatcg gcgaagcgcc agccatgcag gacgtgttcc gtattatcgg
    14341 tcggctttcg cgttcttcta ttagcgtgct gattaacggc gaatccggca ccggtaaaga
    14401 actggtcgct catgccctgc atcgccacag tccgcgagcc aaagcgccat ttatcgctct
    14461 gaatatggcg gctatcccga aggatttgat cgaatcagaa ctgtttggtc acgagaaagg
    14521 cgcgtttacc ggcgcgaata ccattcgtca ggggcgtttt gaacaggctg atggcggtac
    14581 attatttctc gatgaaattg gcgatatgcc gctggatgtg cagacgcgtt tgctgcgcgt
    14641 gctggcagac ggtcagtttt accgcgttgg cggctatgcg ccggtgaaag tggatgtgcg
    14701 gattatcgct gccactcacc agaatctcga acagcgggtg caggaaggta agttccgtga
    14761 ggatctgttc caccgcctga acgttatccg cgttcatctg ccgccgctgc gtgagcgtcg
    14821 ggaagatatt ccccgcctgg cgcgccattt tttacaggtt gccgcgcgag aactgggcgt
    14881 agaagcgaag ttgctgcatc cggaaaccga agccgcgttg acgcgtctgg cgtggccagg
    14941 caacgtgcgc cagctggaaa acacctgccg ctggctaacg gtaatggctg ccggacagga
    15001 agtgttgatt caggatttgc ctggcgaact gtttgaatca acggttgcgg agagtacttc
    15061 gcaaatgcaa ccggacagtt gggcgacgct tcttgcgcag tgggcagaca gagcgctacg
    15121 ttccggtcat caaaatctgc tttccgaagc gcagccagag ctggagcgga cgttactgac
    15181 gaccgcgttg cgacatacgc aggggcataa acaggaggcg gcgcggctac tcggctgggg
    15241 ccgcaacacc ctgacgcgta agttaaaaga gctggggatg gagtgattca cggcttgtgt
    15301 gtaaagattg attattgagc gcaaattgct ggtattttgc gctttactgt tccgataagt
    15361 tcagtatgat cttgcccgga aaacggggag agtcattatg ctggaatcaa taattaatct
    15421 ggtatcgagt ggcgcagttg acagccacac accgcaaact gctgttgctg ccgtgctgtg
    15481 tgccgcgatg attgggctgt ttagttgagt ttcagattgc tgatgaagtg ctctttgttc
    15541 atgccagatg ctgcgtgaac gccttatccg gcctacaaaa gcaatatttt caacaggttt
    15601 tgtaggcctg ataagcgtag cgcatcaggc aatcttgttc tcagcacatt gatggcgctt
    15661 tcgttttcta ctttgtaaac gaagcgccat tcactattta aatcacccga gagaactgct
    15721 gcatccgcgc tttctggcgc agataggtat caaagcacat gcaaatgttg cggatcagca
    15781 agcgaccttt cgccgtcacc tgtattccct tctcatccac atccaccagc ccatcttttg
    15841 ctaacggggc gagcagcttg agatcttccg caaagtaatc agcgaagtgc aaatcccact
    15901 gtttctcaat aggggcgtaa tccagacgga agttgcagat gagcgactta atcacatcgc
    15961 ggcgaataca gtcatcacgc gttagcgcaa taccacgcca cagcgcattg ccttgttcat
    16021 ccacttgctg atagtactgc ttcaactctt tctggttctg cgcgtagcag tcgccaatca
    16081 tgctgatggc ggaaacgccc atccccagca gatcggtatc gccctgagtg gtgtagccct
    16141 ggaaattacg atgcagcacg ccttcacgct gggccaccgc cagctcgtca tccggacggg
    16201 caaagtgatc cataccgata aactgatagc ccgattgcgt caggaaggcg atggtttcct
    16261 gcaggatatc gagtttttgc tgcggactcg gcaggtcagc atctttgatt ttgcgctgag
    16321 cagcaaaaat ggtcggcaga tgcgcatagt taaagacgct cagacggtca gggtttagct
    16381 ccgccacgcg tttcagggta aaggcgaaac tttccggcgt ctgtttcggc aggccgtaaa
    16441 tcaggtcgat gttggtggag gtaaagccaa tctcacgcgc atggttaagc agcgcaaaga
    16501 taaactcttc atcctgctcg cggttaacca gacgttgcac ttctttgttg aagtcctgca
    16561 cgcccatact caggcgatta aagccctcgg cgcgtaaatg atcgagtaca tccagttcga
    16621 tttcccgcgg atcgacttcg atcgaaatct ccgcatcggc attgaactgg aagttttcgc
    16681 gcagcagctt catcaggcgg ctgatttgcg ctttattaag atacgtcggc gttccgccgc
    16741 cccagtgcag ctggctgacg tgacgcccgg cgaacagcgg tgcacgatgg acgatttctt
    16801 gctccagcgc gtccagatac tgatcggcct tgtgctgctg gcgagtaaca atcttattgc
    16861 agccgcagaa gtaacaaagc ttatggcaga acgggatatg tacgtagaga gataatggac
    16921 gctcaggata gcgcgccacg gcttgtaaaa acgcctgttc gccgaagtct tctgaaaact
    16981 ccagcgcggt cgggtacgag gtgtatcgtg gcccggaata gttatatttc tggatcaggg
    17041 ccagatccca gtcgatttgc tgtacagaca tgctcactcc ttccgatggc gtctctggcg
    17101 gctacggcgt accggcggat gcccgtttcg ggtcatcagc cggttgcgca gccacttttg
    17161 tcgccgcgac aaccgctgta gtttaacgaa taaccacacc agataacata taaccggaag
    17221 ggttatcagc agaacgggaa ggcccacggc ggcaatccgt tagttgcccc gcagcagacg
    17281 catcatgtct tcttgcttct cgtcttcttc ctcttcttcg tcatcgtcat aagagaggcc
    17341 gagtttctgc atcaactcat caatacgatc cagtttagca tccacccagg attgctcttc
    17401 ggcactcagg gtttcgcctg cttccagacg ttccagcagc gcatccagac gctcatccgt
    17461 ttctagtaat tccaactccg cctgcggtga aagcataggt ttctcactct tcggtttgtg
    17521 ctgtttggtg actttttcag tcacgcccaa tggaataggg gttttactgc caatacgtgg
    17581 atctttcggt gcgttctggc ctttgctgcc tgacgtgttg tttccgcccg ctgcgcggct
    17641 gcccggcgca tgaccacgac gttttttctg acgcttgcgg tcacgagctt cctgattcag
    17701 ctcttcgcgc gttttgcgac gcgcttttgc atgacctttg ctgcgtgatg gtgaagatgg
    17761 tttcataatg gttcgtcttt tagccgtttt attcagtata gatttgcggc ggaatctaac
    17821 agaaagcaag caaagaaaaa aggcgacaga ttactctgtc gccttttttc ctgactcata
    17881 acccttaacg ggtcttacaa tccttgcagg ccgccaacac accctgtttt tccattagcc
    17941 ggaacaatcc gtgttgattc catttccgtt taattacgtc tccggacgtt tgtcttcctg
    18001 acaatcctct gtcttcgcct cctggcgctc ctgaccctca tcctgagtca ttgttcctgt
    18061 tagcgtcctc gctctatggg tgctacttta cgcctttgct ttaaacaaac aatcaaccgc
    18121 catcagtatt tgcacatttt cagagtttta tataaaagca attaactgat ttatatagct
    18181 tatttgacat gcatcgcaga attttctctg ctatttcgca taaagactat cggcaatgtc
    18241 tcacaaagcg tatggctttt acttacaacg ctcacgccga aaaatccgcc ttttattatg
    18301 gttcaggtat aatcgccgca aagctttgac tgacggagac gaccgctttg actaatttga
    18361 attatcaaca gacgcatttt gtgatgagtg cgcctgatat tcgccaccta ccttccgata
    18421 ccggaattga agtggctttt gcaggccgtt ccaacgcagg taaatccagc gcgctgaaca
    18481 cgctgactaa ccagaaaagc ctggctcgta cctcaaaaac ccctggacgc acccagctta
    18541 tcaacctgtt tgaagtggct gacggcaagc gtctggttga cttgcctggg tacggttatg
    18601 cggaagtccc ggaagagatg aagcgcaaat ggcagcgcgc gctcggcgag tacctcgaaa
    18661 aacgtcagag cctacaaggt ctggtagtgc taatggatat tcgccatccg ctgaaagatt
    18721 tggatcagca gatgattgag tgggcggtag acagcaatat cgccgtgctg gtactgctga
    18781 ccaaagcgga caaactggca agcggcgcac gtaaagcgca attgaatatg gtgcgtgaag
    18841 cggtgctggc gtttaacggt gatgtgcagg ttgaaacgtt ttcttcgctg aagaaacaag
    18901 gcgtggacaa gctgcggcag aaactggata cctggtttaa cgagatgcag cctgtagaag
    18961 aaacgcagga cggcgaataa ttttcttacc ttaatgctta tgccggatgt ggcgtatccg
    19021 gcccataaat tcaatagatt acaattctac cgttggcctg ataaacatgg tgcatcaggc
    19081 attacggatc tttcctttcg cccaataaaa aacgccccag tcattactga ctggggcggc
    19141 taaaatattc agccaaatcc gattacgtga agtaaaaggt ctgaaagata gaacatctta
    19201 cctctgtacc ctacgcgact aactttactc ttttttgttc agttcagaaa gcactttttg
    19261 taattttttt tcagttgttg cataggaatt tctaatagcc atcacaaaac gtgacagctt
    19321 atgttgctta cttacgaaaa aagggcatgt tcaggcgaat cttagtgcgc ctgatcccag
    19381 ttttcgccac tccccacttc caccagcaac ggcacatcca gacgggtaca gttttccatc
    19441 agttgatgaa tctgcttcgc aacggcatcg acatcatctc tatgaacttc aaataccagt
    19501 tcatcgtgta cctgcatgat catacgtaca cgcggttgct cagcctgtaa ccacgcatca
    19561 acggcaatca tcgcccgttt gataatgtcg gcggcggttc cctgcattgg cgcgttaatg
    19621 gctgcacgtt cagccgctgc acgacgagca ccattgctgg atttgatatc cggcagatac
    19681 agacggcgtc cgtccagcgt ttcaacgtag ccctgctctt tcgcctgagc acgggtgcgt
    19741 tccatatact ccagcacgcc agggtagcgt tcgaagtaaa ggtccatgta cttctgcgct
    19801 tctttacgtg gaatgttcaa ttgccgcgcc agaccgaaag cactcatgcc ataaatcaga
    19861 ccaaagttga tcgctttcgc gctacggcgt tgctcgctgg tgacggtttc cagtggcaaa
    19921 ccaaacactt ctgccgccgt tgcccggtgg atatcttttc cttccgcgaa tgcggtcagc
    19981 aagcctttgt cacgcgagag atgcgccata atgcgcagtt caatctgcga gtagtccgct
    20041 gagacaatca cataatcctc tggcgcaata aacgcctggc ggatacgacg accttcttcg
    20101 ttacgcaccg gaatgttttg caggttagga tcggttgacg ataaacgtcc cgttgcagtt
    20161 acagcctggt gataagaggt atgcacacgc ccggttttcg ggttgatcat cagcggcagc
    20221 ttgtcggtgt aagtcgattt cagcttcgcc agaccacgat actccagaat cacttttggc
    20281 aacggatagt ccagcgccag ttcttccagt acctcttccg acgttgacgg cgcgccaccc
    20341 ggcgttttct tcagcggttt aatgccctgt ttttcaaaca gaatggtttg taactgcttg
    20401 gtggaagaaa ggttaaattc ctcacctgca atttcatgcg ctttcttttc cagctcagcc
    20461 agacgaaggg tgagctcttc agaatgattg tgcagcactt tcggatcgat cttcacaccg
    20521 ttacgttcaa tgcgtgaaag tactggcacc agcggcattt cgatattctc gaagacgttc
    20581 aacggccctt tgtgtttttg cagatccggc cacattttca gatgcaactg caaggtgaca
    20641 tcagcatctt cggcggcgta acgtccggcc tcttcgaggg caatctggtt aaaagtcagt
    20701 tgatttttgc ctttacccgc aatctcttca aaagtgatgg ttttgtgctt caaccaacgt
    20761 tccgcgaggc tgtccatatc gtgacgcccg gcaacgctat tgagaatgta ggactccagc
    20821 atggtatcaa acgcaatccc acgcagctca atgccgtaat tcgccagaat accgcgatcg
    20881 tatttcaggt tttgcccgac cttcagcgcc ttttcatctt ccagcagcgg ttttagcaac
    20941 tcgagtgcac gctcgcgaga gatttgatcg ggcgcatcaa gataatcatg agcaaccgga
    21001 atatatgccg ctacgcctgg ctcgatagca aaagaaagcc cgaccaggtt agctgagatg
    21061 ttatcaaggc tgtcggtttc ggtatcaaat gcaaataccg gcgctttttc cagcttcgca
    21121 atccacgctt tcagtgtttc ttcatcaagg atggtgacgt agttgtcata agaaatcacc
    21181 gttgccgtca cttctggtgc ttcgtctgca acactggttt cctgtggctt cgcggctggt
    21241 tttgcccctt tggcctgtaa ccatttgccc gcttcgacat cagcagtcca gcgtttgaac
    21301 tcatactttt tgaacagccc caacaactct tccgctgccg gttgctgcac ttccagttgt
    21361 tcacaggtca gctccagttc aacgtcggtt ttaatcgtcg ccagctggta tgagagataa
    21421 gcaacttctt tgttttgctc gagcttcgct gccattgttt tcgcgccacg gaagctcaac
    21481 ccagcaattt tttctggctc ggcatacagc gtatccagcc cgccaagacc ttgcagcaat
    21541 gcctgcgcgg ttttttcacc gacgcccggt acgccaggaa tgttatcaga ggagtcaccc
    21601 atcagcgcca ggaaatcgat gatcagttct ggcggcacgc cgtacttatt caccacctct
    21661 tccggtccga ggatggtatt cgtcatggtg ttgataagcg taatatttgg cgtcaccagc
    21721 tgcgccatat ctttatcgcc agtgctgatc agcaccggac gcccggcttt ttcggcttcg
    21781 cgcgccagag taccgataac gtcgtccgct tctacgccag aaaccgccag cagcggcagt
    21841 cccatcgctt taaccatcgc gtgcaagggt tcgatttgtg ctcgcagatc gtccggcatt
    21901 ggcgggcgat gtgatttgta atgttcaaac agttcatcac gaaaggtttt tcccttggcg
    21961 tcaaagacca ccgctgcatg cgtcggttta tattgcatga tcagactgcg cagcatgttg
    22021 aggacaccat acatcgcacc ggtcggctcg cctgcgctgt tagtcagcgg gggaaacgcg
    22081 tgatatgcgc gataaagata agatgaacca tctacaagga taagtggatt ttgggggatc
    22141 tgaaccataa tgtccgtgcc tgtttatcag attatgggta aaggatgcca cagacaggat
    22201 aaaaacatga gtttttcacc gcttttcgtg aaaatctttt gtagatcttc tggatcgctc
    22261 gcaaatcgtc atgtggataa ctttgtgcat aaattattta catcgctttt aatttattat
    22321 catgcttttt acagcatgat gtttgtcttt gtttttcatg cagttaaaaa taaatccatc
    22381 cctaataatt gagataatgg gattttttct aatgtggata gatatgaatt atttttctcc
    22441 ttaaggatca tccgttattt gggtcgtttt ctgctaaaat ctgcgccttg cgcttcttta
    22501 gcgcactgtc tatggctaac cttttgaata aattcattat gacgagaata ctcgctgcga
    22561 taaccctttt gctgagtatc gtattgacca ttttggtcac tattttttgt tccgtcccga
    22621 tcatcattgc cgggattgta aaactattgc tacctgtgcc agtcatttgg cgaaaggttt
    22681 cgcgtttttg tgattttatg atgtattgct ggtgtgaagg tctggcggta ttactgcatc
    22741 tgaacccaca cctgcaatgg gaagttcacg ggctggaagg gttaagtaag aagaactggt
    22801 atctgcttat ttgtaaccac cgtagttggg cagatattgt cgtactgtgc gtgctgtttc
    22861 gtaagcacat tccgatgaat aaatatttcc tcaagcaaca gctggcctgg gtgccatttc
    22921 ttggcctggc gtgctgggcg ctggatatgc cgtttatgaa gcgttattcc cgcgcttatt
    22981 tattacgtca tccggagcga cgtggtaaag atgttgaaac cactcggcgt tcttgtgaaa
    23041 agtttcgcct gcatcccacc accattgtca atttcgttga aggctcccgt ttcacgcaag
    23101 aaaaacatca gcaaacccac tccactttcc aaaacttgtt gccaccaaag gcggcaggca
    23161 ttgcgatggc gctaaatgta ctgggtaaac aattcgataa actgcttaat gttacgttgt
    23221 gttatccgga caataaccgt cagccgttct tcgatatgtt aagcggtaaa ctgacgcgga
    23281 ttgtcgtaca tgtggattta cagcctattg ccgatgagtt acatggcgac tacatcaatg
    23341 acaaaagctt taagcgccat tttcagcaat ggctgaactc attgtggcag gaaaaagacc
    23401 gacttctgac gtcgttgatg tcatcacagc ggcaggacaa gtaaattttg ctcgcctctg
    23461 aatgagcaga ggcgagtgag tattttaatg aacaaaacgt ccggcacgag acataaattc
    23521 ttcttcgctc atctcctgtc cgttaaaaac aactttaccc ggtgtataac gcagctgcag
    23581 cgaggcggta ttgttgtcaa tcgtgattaa ctgaaacatc tgacctaatg tcatcatccc
    23641 gctgatttgt ttatcagcct gcttttgcgc tttttccgca tccattcctt cagataaatt
    23701 aagctgtttt gcggtttctg tgacgacatt aagcggtaac ttcacatcaa aattgagcga
    23761 tttgatatct ttgtttgtgg atgatgaaga tttggctggg tcggcaatac tgatatccag
    23821 attggcattt agttcgccga gtgcgttctt ccatcttacc ggttgtttaa tggtcggctc
    23881 actttgttgt aataacggta aatactcttt gaacaatgca gcattcactt cttgcagagc
    23941 aacttcatcg tttgctaatt cagggtgtgc agcaagctgc ttctgcatcg caatgttata
    24001 ctggatgata aattggcgca ccgctgaggg atcaatagat tcagcaatca atgaccactc
    24061 accactaccg agttgctgat tcccttttgt taacttatca agttcgtagg tcagattgac
    24121 attgatataa tctttgtctt taacgcgatc cagcctggtt tttgcaacga aagcatcgat
    24181 ttgggcaacg tcttccccgt gattgataat gttaatctga ttaaatttcg actcgctttc
    24241 accaaccgga aatttctttt cttctaatcg cgccagagaa tcaatggtaa aactttttgc
    24301 cgttactttg ttcattggtg ataattgcag aacaatatct tcgactttgc cttcaatatc
    24361 caggttagac aatgaactac tatcaccatt aaaagtaaac tggccctcac caaaagaaaa
    24421 ctttccatac tctgcagggg caacatctaa tgtcgtagag aatgagccac caaacgcgaa
    24481 tcgcaatgtc gcttccacgg gcgatttatt tttcgcggcg ataaatagcg gttgtgtcag
    24541 ttcattattc actaagttca cttttgccgc tgccagtgct gggatgacat taccatgcat
    24601 taacattgtg atgggcaacg gaccgtgctc aacatccaca tcaaatacaa ctttctggcc
    24661 tggcttgata ttgagatcgg gtgcaccgtt atcgaatgtc atttgcattt gaaaacggga
    24721 attaaaaaag cctttgtcaa aattttgata acttaacgtc atatcgtagg catgttcacc
    24781 tttcttttta gcatcgttaa aatctttaat aaatttttcg acaccaggct gaatttgcgt
    24841 acctgtgtac catgtgccac caccccagat tacggctaac gcaacaataa cacctgtagc
    24901 tgacttacgt atcatataaa tattccctga taaaacagca aaactttgaa tatccactta
    24961 t
//
LOCUS       DBFIFM010000052        24917 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.052, whole genome shotgun sequence.
ACCESSION   DBFIFM010000052 DBFIFM010000000
VERSION     DBFIFM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.052"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            159..1328
                     /gene="ydhP"
                     /locus_tag="N7C74_001842"
     CDS             159..1328
                     /gene="ydhP"
                     /locus_tag="N7C74_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2431722.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNKFGAAISMAVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(1474..2055)
                     /gene="sodB"
                     /locus_tag="N7C74_001843"
     CDS             complement(1474..2055)
                     /gene="sodB"
                     /locus_tag="N7C74_001843"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HGU2431723.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(2183..2998)
                     /gene="mepH"
                     /locus_tag="N7C74_001844"
     CDS             complement(2183..2998)
                     /gene="mepH"
                     /locus_tag="N7C74_001844"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HGU2431724.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHTVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            3333..3680
                     /gene="grxD"
                     /locus_tag="N7C74_001845"
     CDS             3333..3680
                     /gene="grxD"
                     /locus_tag="N7C74_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HGU2431725.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(3731..8347)
                     /gene="lhr"
                     /locus_tag="N7C74_001846"
     CDS             complement(3731..8347)
                     /gene="lhr"
                     /locus_tag="N7C74_001846"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HGU2431726.1"
                     /translation="MADNPNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAADAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVELVIQVATPLSVASGLQRIGRAGHQIGGISKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQHTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPAILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEIASYLE
                     NLLTVKRIFPTMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVHDYSPEVLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDPSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQAAQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVWQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(8440..9087)
                     /gene="rnt"
                     /locus_tag="N7C74_001847"
     CDS             complement(8440..9087)
                     /gene="rnt"
                     /locus_tag="N7C74_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HGU2431727.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(9190..9597)
                     /gene="gloA"
                     /locus_tag="N7C74_001848"
     CDS             complement(9190..9597)
                     /gene="gloA"
                     /locus_tag="N7C74_001848"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HGU2431728.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(9678..10775)
                     /gene="nemA"
                     /locus_tag="N7C74_001849"
     CDS             complement(9678..10775)
                     /gene="nemA"
                     /locus_tag="N7C74_001849"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HGU2431729.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPSI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(10812..11411)
                     /gene="nemR"
                     /locus_tag="N7C74_001850"
     CDS             complement(10812..11411)
                     /gene="nemR"
                     /locus_tag="N7C74_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HGU2431730.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNYEPLENALAHVKTIIATPAV"
     gene            11514..11753
                     /gene="ydhL"
                     /locus_tag="N7C74_001851"
     CDS             11514..11753
                     /gene="ydhL"
                     /locus_tag="N7C74_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HGU2431731.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11802..12698
                     /gene="ydhF"
                     /locus_tag="N7C74_001852"
                     /pseudo
     CDS             11802..12698
                     /gene="ydhF"
                     /locus_tag="N7C74_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
     gene            12779..13300
                     /gene="sodC"
                     /locus_tag="N7C74_001853"
     CDS             12779..13300
                     /gene="sodC"
                     /locus_tag="N7C74_001853"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HGU2431732.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(13301..15313)
                     /gene="ydhK"
                     /locus_tag="N7C74_001854"
     CDS             complement(13301..15313)
                     /gene="ydhK"
                     /locus_tag="N7C74_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HGU2431733.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSDVDDHARLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYNFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGIVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(15313..16170)
                     /gene="ydhJ"
                     /locus_tag="N7C74_001855"
     CDS             complement(15313..16170)
                     /gene="ydhJ"
                     /locus_tag="N7C74_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGU2431734.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(16173..16409)
                     /gene="ydhI"
                     /locus_tag="N7C74_001856"
     CDS             complement(16173..16409)
                     /gene="ydhI"
                     /locus_tag="N7C74_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HGU2431735.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            16610..17044
                     /gene="slyA"
                     /locus_tag="N7C74_001857"
     CDS             16610..17044
                     /gene="slyA"
                     /locus_tag="N7C74_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HGU2431736.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(17091..17558)
                     /gene="slyB"
                     /locus_tag="N7C74_001858"
     CDS             complement(17091..17558)
                     /gene="slyB"
                     /locus_tag="N7C74_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HGU2431737.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            17831..18940
                     /gene="anmK"
                     /locus_tag="N7C74_001859"
     CDS             17831..18940
                     /gene="anmK"
                     /locus_tag="N7C74_001859"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HGU2431738.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVKALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            19038..19367
                     /gene="mliC"
                     /locus_tag="N7C74_001860"
     CDS             19038..19367
                     /gene="mliC"
                     /locus_tag="N7C74_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HGU2431739.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            19426..20082
                     /gene="pdxH"
                     /locus_tag="N7C74_001861"
     CDS             19426..20082
                     /gene="pdxH"
                     /locus_tag="N7C74_001861"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HGU2431740.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGSWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            20211..21485
                     /gene="tyrS"
                     /locus_tag="N7C74_001862"
     CDS             20211..21485
                     /gene="tyrS"
                     /locus_tag="N7C74_001862"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HGU2431741.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            21547..22407
                     /gene="pdxY"
                     /locus_tag="N7C74_001863"
     CDS             21547..22407
                     /gene="pdxY"
                     /locus_tag="N7C74_001863"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HGU2431742.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(22451..23056)
                     /gene="gstA"
                     /locus_tag="N7C74_001864"
     CDS             complement(22451..23056)
                     /gene="gstA"
                     /locus_tag="N7C74_001864"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HGU2431743.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(23162..24664)
                     /gene="dtpA"
                     /locus_tag="N7C74_001865"
     CDS             complement(23162..24664)
                     /gene="dtpA"
                     /locus_tag="N7C74_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HGU2431744.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQNGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
BASE COUNT         6097 a         6331 c         6542 g         5947 t
ORIGIN      
        1 tcgagggagc gcgttctccc tcgcatctcc ttcctgtttg ttagcaaaat ggcaaaagtg
       61 ttttctcttt ttgttatttt catttttttg tgattgctgt catgctttcg ccactttaat
      121 gaatatcgcg taatgacgcg aagaggtgga gataaggcat gaaaattaac tatccgttgc
      181 tggcgctggc gattggcgcg tttggtatcg ggacaacgga gttctcgcca atgggcttgt
      241 tgcccgtcat tgcgcgcggt gtggatgtct cgattcccgc tgccggaatg ttaatcagtg
      301 cctatgcagt tggcgtaatg gttggcgcgc cgctgatgac gcttctactt tctcatcgtg
      361 cccgccgcag tgcgttgatt ttcctgatgg caattttcac gctcggcaac gtactttccg
      421 ccatcgcgcc ggattatatg accctgatgc tttcacgcat tttgaccagc ctgaatcacg
      481 gagcattttt tggtttgggt tcagtcgtgg ccgcaagcgt ggtgccaaaa cataaacagg
      541 ccagcgcagt tgccactatg tttatggggt taaccctggc aaatatcggt ggcgtgccgg
      601 cggcgacctg gttgggtgaa accatcggct ggcggatgtc atttctggca acggcggggc
      661 tgggagtgat ttcaatggta agtctgttct tctcattacc taaaggtggt gcaggggcac
      721 gacctgaagt gaaaaaagag ctggcggtat taatgcgtcc gcaggtgctg tctgcattgc
      781 tgacgacggt actgggagct ggtgcaatgt ttactctcta cacctatatc tctccggtac
      841 tgcaaagtat tacccacgca acaccggtgt tcgtcacggc aatgctggtg ctgattggtg
      901 tcggattctc tatcggtaac tatctcggcg gcaaactggc agatcgttca gttaacggca
      961 cgttgaaagg ctttttgttg ctgctgatgg tgattatgct ggcaatcccg ttcctggccc
     1021 gcaataagtt cggcgcagct attagcatgg cggtgtgggg cgctgcaacc tttgcggtcg
     1081 taccgccgtt acagatgcgc gtgatgcgtg tcgccagtga agcgccaggt ctgtcttcat
     1141 cagtcaatat tggtgccttt aatcttggaa atgcgctggg agcagctgct ggtggtgcgg
     1201 taatttccgc tgggctggga tacagctttg tgccggtgat gggggcgatt gtcgcgggac
     1261 tggcattatt gctggtgttt atgtcagcca gaaaacaacc tgaaacagtt tgcgttgcta
     1321 acagctaatt aaacaaaaac gcagaaggat aaccttctgc gttacagttg ctgacaacgt
     1381 gcgatttatc ttgccgaatg cgctgttaac gcctcatccg cgttggcatg gtaagcgaag
     1441 cgcatcaggc aatactgcat ttgccatcag ttattatgca gcgagatttt tcgctacgaa
     1501 ttcccagttc accagcgccc agaagtgctc aagatagcca ggacgtgcat tgcgatagtc
     1561 gatgtaatat gcgtgttccc agacatcaac ggttagcagc ggagtcgcat cggtggtcag
     1621 cggagtaccc gcgttagagg ttgaaacgat agccagtttg ccatcgctgt ttttcaccag
     1681 ccaggtccag ccagaaccaa agtttttgat cgctgcatca gtaaactgag ctttgaaatc
     1741 ggcaaagctg ccaaaagatg cggcgatagc ttcagcgact tttccagtcg gttcgccacc
     1801 ggcgttcggt gccaggcagt tccagtagaa agtatggttc cagacctgag ctgcgttgtt
     1861 gaatacgcca ccttcagagc tgcgaataat ctcttccagt gatttacctt caaacgcggt
     1921 acctttaatc aggttgttca ggttagtgac ataagtctga tgatgcttgc cgtagtgata
     1981 ctcgatagtt tccgcagaaa tgtgcggtgc cagagcatct ttagcatatg gtagtgcagg
     2041 taattcgaat gacattgcta ctctccttta ttattaattt gcatacgtac aatagcctta
     2101 ttgtgcgtat ggatagggta gcaaattaaa aggtgagaca aaagatgaac ttaccctgtt
     2161 gccgtaacaa cagggtaaag ttttagcgaa gtgtttttgg ggtcattacc cggcgagcgc
     2221 caacatagtg gcgctgccag tagtcttcac tgagagaagt gatttgaatt tcctgacctg
     2281 tgcgcggtga ctgaataaat ttgccgttgc cgacatacac gccgacatga tcggctgtac
     2341 cgcgtccctg agtacggaaa aagaccaggt cgccgttttt cagttcacta cgttcgattg
     2401 gcgctgcatc gcgcaggtga tacatttcat tcgccgtacg cggaatacga attttcacca
     2461 aatctttata agcgtaataa accaggccgc tgcaatcaaa accggtacgc ggtgagctgc
     2521 caccccaacg atatggctta ccaatttgct gcatcagttt attcattgcc acttttgtgg
     2581 ctttttgcac cttcgctttg tgtgcgtcgg caagggttcc tgacgctgca tttttgactt
     2641 tcacacaatg cgatttatag cccttacgtt tggtacactt ttcggtaaag ctggctgagg
     2701 ccgttttatt gacggtatgt ttgctgcgag ttttggcgtt tttagaagcg gtaatggaag
     2761 attttttaac ggaagaagcg gttttgcttt tggtcgtact tttactggcg gcttttttcg
     2821 ctgttttctg ggtctttttg gtggttgccg tgcttttctt tttatctgct tttttggtga
     2881 tatgagtggt agcagaactc tccctggctt gctttgaagc atgggccata ggcgttaaag
     2941 gcagggtggt aaaaagtaaa gcacagagcg tgatcgaaat acggtttatc cgcgccactg
     3001 agcgtccctc tgttaaggca ggtctatcat gcctgcgtct gatttacaaa acttgatgat
     3061 tctaatgcat agaagaagag aaagttaata gcttttattg ttaaaaaata cgtttcgttg
     3121 gcgagcgcaa aaaaatgcag cagtgaaagc taaaaatggt cataaatacg ccagtttgcc
     3181 gtgcatcagg gaagaaacga atatttgaaa tgcaacaaat agagcaaagg gataaaatag
     3241 caaaagcgtg atgaaaatgt tattttccga tgccgcactg acccgctaca atgtcagatt
     3301 actgccgtaa agaagtttga ggaagcaaga caatgagcac cactatcgaa aaaatccaac
     3361 gccagattgc tgaaaacccg atcctgctgt acatgaaagg ttcaccgaaa ctgccgagct
     3421 gcggtttctc tgcccaggca gtccaggcgc ttgccgcatg tggcgaacgt tttgcctatg
     3481 ttgatattct gcagaatccg gacattcgtg cggagctgcc gaaatatgct aactggccga
     3541 ccttcccgca actgtgggtt gacggcgagc tggtaggcgg ttgtgatatc gtgatcgaaa
     3601 tgtatcagcg tggcgaactg cagcagctga tcaaagaaac tgccgctaaa tacaagtctg
     3661 aagagccgga cgcggaataa tccgtcctgc aatactgacg gatgcgaacg ggcatccgtc
     3721 agtcctttct ctatccccaa tccagccctt gtggcgaact tgaaaatccc gcttcacgca
     3781 gcagcgtaaa catcggcgtt tgccagaccg gtagatcatt cacttctgtt agcgtaaagc
     3841 gcagccgtgg ttcgcgacgc agtgcggtag tcagcgcgtg gaaaacctct ggggcgagta
     3901 attcctcttt ttcctgccag accagcattt ttttgccacc ttgcgccaga tagagtaaca
     3961 atttgccgcc agaaacgacc accagcgcac ccgcccgacg cgttggtacc agcgttgccg
     4021 gatgtgcagg ccagggcaga agatttcccc acacattagc cggatcgttg gcggagagcg
     4081 ccactggtgt atagtggcgc gtttgcgcgg cttgtgtcgc caggtcgcgc aagcggtcaa
     4141 tagtcagacg ttcagcgaat tgcgcgccac ccagaccttc tacaaaacga cctcgcataa
     4201 ttcgcccgga atcttccata cttcgacaaa gcgtttgcat cgatggaaac ccgccaggga
     4261 tattttcggc tatcaccgcc tgacgactga tgatgccgta gcggtcgagc atattttccg
     4321 ccagcgccag catcctttcg gtatcgttta gtggctccac ctgcaataac gaccagcgtc
     4381 cagccagatt tggtgtgttg taagataccc gcggcgagac gggttgtgca tagacaggac
     4441 gcccacgacg agcccggtga ctgcggcgag ttgaggtgcg tgcgttggaa ctgctgcggg
     4501 tgagggcgcg taacggtgcc caaatatcgc tggtgatgac gccttgccag accagcgccc
     4561 ataacgcctc ttgcagtgcc gagggatcaa ccgattcgcc gattttgtcg cgtatccgtt
     4621 ggctgatttg ttgtgcaaac caggctcctc cgtcagccag aacagcgact atcgcttgtt
     4681 gcagcgccga acgattcgcc tgatccgctt cggcgggagt gaacgattct gcagcatatt
     4741 cctgtagatg cagtgccacc aggccgtcat cttcacccag ttttttttgc cccgaccaga
     4801 taaccgcacc ggttgccagt aattcatcga gcacttctgg cgaatagtcg tgtacgcggg
     4861 caggcaagat ctggctttcc cagagtgagg cgggtaagcc gactccggca agctgttcga
     4921 tcacccgcat cacgccatcc accccctcat aaacgcctgg cgatgttgag gcaaagagcg
     4981 ccgggctacc atcggtggcg ggtaatacgc cctgacgttc cagcagcaat cgcgcatagg
     5041 tcgtggctgc aacgggacgc gtcgcttctc tggcggcttg cagcgagcgc aaacgcagac
     5101 gacgaaatac ttcatcgctc acccagatgt cttgttgcag attcatcacc agaccctgtt
     5161 cgcgcagttg ctgaagctgc tcttcgacaa tggcaatacc gagactaaac tcatgagcca
     5221 gttgttcagc cgtgaccaga gcatgagccc ggagatagcg tagaaagagg tcgcgaagcg
     5281 ggtaactgac tctatgcaaa taaatctctg gcagtgactc tggtaatcgt acgccgaggg
     5341 catcgcgcag cctggcggca tcatccatac aggcaagacg ctcctgtcca ctaatcatcg
     5401 tcgggaagat gcgttttact gtaagaagat tttccagata cgaggcaatc tcttcactgc
     5461 tgcctgtatg ccgttgcgcc aggtcttcaa cggtcattgg ccccagttcg cgcagcaggt
     5521 cgaacaatcc ttcttcacct ttcgctcttc tgccaggagc cagtcgttgc aactcttctt
     5581 ccacctggcg aatgacctgc gggtcgagta attcccccgg atcgacctgt ccgagtagat
     5641 tgcgcagtaa ctcgctgtcc agcgacagta cggatgcccg gcgttctgcc agcggggcgt
     5701 cgctctggta cataaattcc gcgacatagc cgaacaataa acttgtggcg aaaggcgagg
     5761 gcgtagtagt cgttacatcg gatatttgaa tttcgcctcc gttcaggcga cgcatcaaac
     5821 gttccagtgc gggaagatca taaacatctt gcagacattc gcgtagggtt tcgagaatgg
     5881 ccggaaaatc cggatatccc tgagcgattt ccagcaactg actggcgcgc agccgttgtt
     5941 gccaaagcgg ggtgcgatgg cccggcgtgc ggcccggcat taataatgcc cgcgcggcgc
     6001 attcgcgaaa acgggcggcg aaaagtgccg aactgcctac cgcctcgcgg acaatttgca
     6061 gcaacttttc tggttcaaac aaaaaaatcg cggcgtcggg caatttacca tcagtgtcag
     6121 gaatgcgcgc aacaatgccg tcatcactgg cgaccacaga cgcgtcagcg ccccaaagcg
     6181 catgtattcg cccggcaatc gccagcgccc agggttcatg cacccgtctt ccatagggag
     6241 agtgcaaaat aatacgccaa tcgccaattt catcacggca ccgttcgagg accagatgac
     6301 gactccccgg aacaataccc gtcgcgttgc gctgctcgtc aatcaacccc tgaatattgg
     6361 cgatcgtatt ttcttcagcc agccacgggg gaatagtccc ggaaaagaac gctccatccg
     6421 ccagcaaatg aagaaaatcg ccgatcatct cgcctaattc agccggacgt ccgttacctt
     6481 caccacgcca gaaggggagc cgggcagaac gacccggagc aggagtcaca atcacctgat
     6541 cgcgggtgat ttgctggatc cgccatgagg ttgcgccgag agtgataata tcgttcaccc
     6601 gcgactcata taccatctcc tcatccagtt cacccacccg tcgcgaaccg gcctgttctt
     6661 ccccttcggg taataacacg ctatacatgc cacgatccgg aatggtgccg ccgctggtaa
     6721 ccgccaacag ctgagcgcca ggtcgggcgg tcaatatccc ggtctccctg ttccagacca
     6781 gtttggggcg aaaagcagaa aaatcgccag agggatagcg tccggaaagc atatccagcg
     6841 tggcgtcaaa aacacgtctt ggcagatctt tccacggtgc ggcacggcgt acgcgggagt
     6901 accattcgtc tacctgtaat gcatccatcg ccgcggcggc aacggtgtgc tgcgcaagga
     6961 cgtcgagtgg attatgcgga ggtgtcaggt tttccagcct gcctgcgaac atacactcta
     7021 caatgactgc ggaatcgact aagtcacgcc tggttcgggg gaaaaaaagc cctttggaga
     7081 tacctcctat ctggtgtccg gcacgtccaa tgcgttgtaa cccactggca acagaaagcg
     7141 gcgttgccac ctgaatcacc agctccacag cgcccatatc aatccccagt tcgagactgg
     7201 aagttgcgac cacgcagcgt aactcacccg atttcagcgc ctgctcggtg attgctcgtt
     7261 gttctttaga gacggagccg tggtgtgagc gggcaataaa aacgtcgcta ctttgtacac
     7321 ggttagaggt tgcgccagag gtcgactcga aatgggccgc atcagcggcg atagacgggg
     7381 aacgctgtaa gcgtgcggcg taaagctcat ttaatcgtgc cgtcagtttt tccgccagcc
     7441 cccgcgaatt agtaaagaca atggtcgagc gatggcgcaa cacttcatca aggatacccg
     7501 tttcaatata tggccagatg gagccttccc ggccggcatg gctgtcttcg ccagtgccgc
     7561 tggcgaccga tgagacatca tccatattgg cgactggtac gacaatccgt atctgcggat
     7621 gacgcattgc gggcgggttg actaccgtaa ccgggcgatc gccaccaaga aatgctgcca
     7681 catcgctggc tgagcgcaca gtggcagaaa ggccaattcg ctgtgctgag gtgtggagca
     7741 gcgcatcgag ccgctccaga cttaacgcca gatgcgcacc acgtttactt cccgctaccg
     7801 cgtggacttc atcaataatt accgtttcga cgccgcgtag cgtttcgcgc gcgcgggagg
     7861 tcagcatcag atagagtgat tcgggtgtgg tgatcagaat atccggcgga ttacgggtga
     7921 gtttgctgcg ttcctgtgca ggcgtatcgc cagtacggat ccccacgcga agattgactt
     7981 ccgtttcgcc gcgccgccgc cgttcatcgg caataccctt caacgggatc tgcaagttgc
     8041 gctgaacgtc ggtgcccagg gcttttatcg gtgaaatata gaggatgcgt gaggttttac
     8101 gtttatgcgc ctcgcgggta tcttcgccgc cttcgcggaa gagtcgatcg agggcgtaga
     8161 gaaatgctgc cagcgttttc ccggagccag tcggtgcaat caccagcgca tgttcgcctc
     8221 gcgccgccac atgccaggtt tgcgactgga cagcggtcgg ctgtttaaag gcgcgaagaa
     8281 accagtcgcg ggtcgccggt gaaaacacgt ccggcagggg cgatgaaggg tttggattat
     8341 ctgccattaa cgctattccc cgtgaatatc atgcatgatg cgtggaatag cctcactata
     8401 gatgcctgaa tcattgcatg tcatcaggca tcgactcgat tacacctctt cggcggcagg
     8461 tagcggccag cctcccagac gtttccagcg gttgacgatt tcacaaaaca gcaccgcagt
     8521 gcgttcggtg tcgtacagcg cggagtgcgc ctgggtgctg tcgaagtcca tgccagcggt
     8581 ctggcaagcc tttgacaata cggtttgtcc gagtgccagc ccggccagtg cagcagtgtc
     8641 aaaagtggcg aaagggtgga acgggttacg tttcagtgag gcgcgttctg cggcggccat
     8701 cataaagctg tgatcgaaat tggcgttgtg cgccaccata atggcgcggt tacagccgct
     8761 cgctttaata cctttacgta caactttaaa aatttcgtgc agcgcctcgt attcgctgac
     8821 cgcgccgcga tcgggatcgt tcgggtcaat gccgttgaaa gcaagggctt ctggttgcaa
     8881 atttgcgccg acaaatggtt cgacgtggaa atgtaatgtg gtgtccggca tcagccagcc
     8941 ttgttcatcc attttcaggg tgatggcggc aatctcaagc agcgcatcgg ttttggcgtt
     9001 aaatccagct gtttcaacat cgatcacaac aggataaaag ccacgaaaac ggtcgcacag
     9061 accggtaagt tgagcgttat cggacatctg attctcttaa tacggataaa ttgcagcgcg
     9121 cattatgaca aatattactt gtcgatgcag taaagatgcg ggcgcgatga gttcgcgccc
     9181 ggcaggagat tagttgccca gaccgcgacc ggcgtctttc tcttcgatta actcaatttt
     9241 gtaaccgtcc ggatcttcca caaacgcgat aaccgtagta ccgcctttta ccggacccgc
     9301 ttcacgggtc acgttacccc cgttttgacg gattttttcg cacgcttcag cggcattatc
     9361 tacgctaagc gcgatgtgac cataagcagt gccgagttcg tatttatcca cgccccagtt
     9421 gtaggtcagt tcaatcaccg cttcttcggt ttccgggccg tagccaacaa acgccagtga
     9481 gtatttgtat tccgggtttt cgctggtacg cagcagtttc atgcccagca ctttggtata
     9541 aaaatcgatg gagcgttgca gatcgccaac gcgcagcatg gtatgaagaa gacgcatctt
     9601 tttatcctca aaatggctaa cagattcaaa atgttgtttt agtatagcgg cggctttacg
     9661 ccgctcgcaa tgttggatta caacgtcggg taatcagtgt agccttccgc accgccacca
     9721 tagaaacttt cagcacgctg tgggttaagc tcagctttgc gctgcaagcg ggcgaccaga
     9781 tccgggttcg caatccagtc acgaccaaat gccaccgcat caattaaccc tttgccgatc
     9841 agcgtttcag ctttttctac tgtgtatgca cctgcgccga taatcggacc gtggaaacgg
     9901 gcgcgtactt tttcgcggaa cgcatcagta tacggttcac cccccgccca atctggttct
     9961 gacatatgca gataagcaat gccgcgttta cccagttgtt caatcagata cagtgcatcg
    10021 gcttcttcat tcgggccgtt atccgtgttc tggaaagtac cgattggcga aacgcgaatg
    10081 ccaatgcgat cggcacccca ttcttcaatc ccggcatcga ccacttccag taccaaacgt
    10141 gcgcgatttt ccacgctgcc gccgtactga tcggtacgat ggtttgaaga aggagaaagg
    10201 aactgatgca gcaaataacc atgagcagag tggagctcta ccagatcaaa accggcttca
    10261 cgcgcgttag caatagcctg acggaaatca ttgacgatac ttggaatctc ttccagttca
    10321 agcgcacgcg gcatggatgt ttcaacacgg atcgcctgac cattttcatc gcgcagagaa
    10381 gtacgtgttc ccgcgctaag tgctgaaggc gctactggtg cctggccgcc aggttgcagg
    10441 ctggcgtgag aaatgcgtcc ggtgtgccac agctgtacgg ccatatgccc attttcagca
    10501 tgaacgccag cggtaatttt tttccatgcg gcaatttgtt ccggactatg gatgccaggt
    10561 gcaccagcat acccttttgc ctgggcagaa atttgcgtgg cttcgctaat aatcaaaccg
    10621 gcactggcac gttggcgata gtattccgcc atcaacgggg tagggatgtc acccggttca
    10681 atactgcgca gacgcgtcag cggtgccata aaaatacggt ttgccgcagt tattgcgccc
    10741 actttcagtg gggaatacag tttttcagat gacataaagg ctcccggtcg tctggtgata
    10801 aaaaatgcct gctaaacggc aggcgtcgca ataatggttt ttacatgtgc cagcgcgttt
    10861 tccagtggct cgtaattgcg cgaaattttg gcctgcagat tcgcacccag ccatagtgcg
    10921 taaagcactt gtgcctgttg cagcggttcg ccacaaaagg ttaaacaatg gctatcacgg
    10981 ccattttcca gagcctgcga gagcagggcg atcacgccgc gagcgccttt atccatcgcg
    11041 ctacgcatat cttctgacag atcgcacact tcggcagaga gttttactgt caggcaacca
    11101 ctgatggttc catgttggca aaactggttc agtgtttgct ggtaataagc cagtatgcgg
    11161 tcgcggtagt taccttcgcc ggattgcagc aactcagtca gtcgttggtg atatgtggcg
    11221 taatggcgct caagcatggc aacgccaaac gcttctttag agcgaaagta gtgatagaag
    11281 gaccctttcg gcacttcagc ggtttttagt aattcgctta gccccatccc ggtgaatcca
    11341 cgttgcaggc aaagttgctc gcccgtcgcc aggagatgtt cgcgagtatc atgttcggtg
    11401 tgtttgttca tgcgttggag tgtagtagac cagtcggtct agtgcaagca aagttgctac
    11461 tttgggcatt aacagcttca ataatggtaa taatcttatt tgaggagggc gaagtggcgg
    11521 agcaattaga gttctttccc gtccagagcc cgtgccgggg aatttgccag tctgacgaac
    11581 gcgggttttg tcgcggctgt tttcgaagcc gggatgagcg ttttaactgg aataaaatga
    11641 gcgatggcga aaaacaagag gtgctgcgcc tttgtcggca gcgattaatg cgcaaattac
    11701 gtgcaaataa gccagcgtca tcagacgaac cggagcaacc atcactcttt tgagcctaat
    11761 cttgcgtata ctcctctcat atctgataag aggaagcgat tatggttcag cgtattacta
    11821 ttgcgccgca aggcccggag ttttcccgtt ttgtgatggg ctactggcga ttgatggact
    11881 ggaacatgtc cgcccgccag ctggtcagtt ttattgaaga gcatctggat ctcggcgtga
    11941 ccaccgtgga ccatgctgat atttatggtg gctatcagtg cgaagcggcg tttggcgagg
    12001 cgctgaaact ggcacctcac ctgcgtgagc ggatggaaat cgtcagtaaa tgcggtatcg
    12061 cgacgaccgc gcgtgaagaa aacgtcattg gtcattacat cactgaccgc gatcacatca
    12121 ttaagagcgc cgaacagtcg ctaattaatc ttgcgaccga tcatctggat ctgctgttaa
    12181 tccaccgccc ggacccgtta atggatgccg atgaagtggc ggacgcgttc aaacatctgc
    12241 atcagagcgg caaagtgcgt cattttggcg tatccaactt cacgcctgcg caatttgccc
    12301 tgttgcaatc acgtctgccg tttacccttg ccactaatca ggtggaaata tccccggtac
    12361 atcagccgtt actgctggat ggcacgctcg accaactgca acaactgcgt gttcgtccga
    12421 tggcgtggtc ctgccttggt ggtggtcgtc tgtttaatga tgattatttc cagccgctgc
    12481 gtgatgaact ggctgtggtg gcggaggagt taaacgcggg ctcgattgaa caggtggttt
    12541 acgcctgggt attacgttta ccgtcgcagc cgctgccaat tatcggctga ggtaaaattg
    12601 agcgcgtacg ggcagctgtc gaagcagaaa cactgaaaat gacccgtcaa caatggtttc
    12661 gtatccgtaa agcggcactg gggtacgacg taccgtaagc acttttagga atagccgccg
    12721 ttcaaaaatg tgtcactggt ttacacttat tcaggaatgc acaatgaacg gaggtcctat
    12781 gaaacgtttt agtctggcca ttctggcgct ggttgttgcg accggcgcac aagctgccag
    12841 tgaaaaagtc gagatgaacc tcgtcacgtc gcaaggggtt gggcagtcaa ttggtagcgt
    12901 caccattact gaaaccgata aaggtctgga gttttcgccc gatctgaaag cattacctcc
    12961 cggtgaacat ggcttccata ttcatgccaa aggaagctgc cagccagcca ccaaagatgg
    13021 caaagccagc gccgcggaat ccgcaggcgg gcatcttgat ccacaaaata ccggtaaaca
    13081 tgaagggcca gaaggtgccg ggcatttagg cgatctgcct gcactggtcg tcaataatga
    13141 cggcaaagct accgatgccg tcatcgcgcc tcgtctgaaa tcactggatg aaatcaaaga
    13201 caaagcgctg atggtccacg ttggcggcga taatatgtcc gatcaaccta aaccgctggg
    13261 cggtggcggt gaacgctatg cctgtggtgt aattaagtaa ttaagaggcc agcgtacctt
    13321 gcggtggtgc ttgctcaagt tgcgaaagcg agcagtacag ccgccagaca attgccgcca
    13381 gctcacgggc ggcaggttga tgatggcggg caaggctgtc gcaaatccgc tgtaattctt
    13441 caagtgtggc cgccagtgat ttttgctgaa cgccacgctc actcatcact ccccgcaaca
    13501 gtgaaataca gttatcccgt actcgcgata acggatcgga acgcgattcc cagtcgcgta
    13561 attgccagac aacatgagaa cagttcagca gcactatacc ccagcgtaat aaccaacggc
    13621 gagccagcgc atcctggctg ttactcaact gactgacgtg atgataagtg agcgattcaa
    13681 attcgctttc actcagtgtt ggatggcggc ttagttgatc gacaaaatcc cggcgcagcg
    13741 cgcgaatatg gcggcggctt ttgcgagcat ccgatcctgg acgcagaatg gcgaacgcta
    13801 accacgccaa cgcgacgcca acgatttttg ccagattatc gttaagaaaa tcagcaaaat
    13861 tatacaccgg gggattagtg acggcgataa aagaacccat aaaaacaatc agttgccccc
    13921 acaatgcggc aaatttgggc atctgcaatt taagaagctg cattgtcgcc agcagtggaa
    13981 agagaaacag taaaaattgc cacagatcgc taatctggac catcagacca aatttgacca
    14041 caaagctgaa tagcgaaagt aacaccagcg tgcgcatcag cagtgacaac gacttaaacg
    14101 gtgctgcgac ggcggagtag agtacgcagc taatcgctgc cagcgttaat gcattggcac
    14161 cggcatccca ttgcgaagca atactccatg cgccaatcat catcaacgta caaaatgtac
    14221 gcagtccgct ccacatagct tcggcgttat cggtatgacg agccaggccg gatgtccgtg
    14281 ggagtctggc atgatcatct acatcgcttt gcagacgatg taattcctga ctactttgca
    14341 gataaaggcg gcaaaaataa cgtagtcgct gccagaaggc gacgtgccga tagtcggcga
    14401 cgttggtcgg gcgtagcggg gcgataatac gtgcgacggt gtaaacatct gtttgcgaac
    14461 tggcgagcgc cgtcagcaac tgttcgagaa tttctcgcgt ggcacctggc ggtgagggcc
    14521 agttgagcaa catacgtcgc aggctggaga tgacactggt catacgtaat tgctggtgga
    14581 gcagcgcatt aaggcgcgcg ttttgctggc gaaaacgata gtggctccag aaagcctgga
    14641 tacgcagcaa attcatggtc agtatctgcc caatcacccc ttcatgtgct gcacgaatgg
    14701 catcggttgt ttcaggctgc cagagtaaac tggcatgttc cagtaatcgg gcgtgcatgt
    14761 ttttcaatgc ggttaaaagg gctgtagcat cggaactgct aggcaggatc atcatcatca
    14821 tgccgccgca caaaataccg acaatcacct cgcaaacgcg cgcctgagcg atatcccaca
    14881 gctggctggc ctcagtaata ttaaccatcg gaaaggcgat gatggcagcc gtgtagcccg
    14941 ccagttgaaa tgcatacgcg acgttattcg tgaagtgcgc acaggcccag gtacaaaagc
    15001 caagccacgc cgacatgctc aatagaaaaa accacggctc attgagcgta tgcccggcaa
    15061 gaagcagtgc cgcaatggct cccagtaaac tgccagcgat gcgtccgagg cttttgctga
    15121 tgacaccgcc aacggtggga aagctaacca ctgcagccga ggtcatcgcc caatagggtt
    15181 catccagatt caaataatag gcaaccgtca gcgccagaca catggcaatg gtattgcgta
    15241 acgcataacg ccattgcgcc agcgtggccc tgaaccaggg caaattgcgc aaggaccatg
    15301 acgatgcgtt cattattgac ctacggcaat gctacaggtg gtgcccgaca ccagcgtgac
    15361 atcgcctgga actttatcca gcgcaaagcg gacgggaacg cgttgggcga gacgaaccca
    15421 ggggacgtta ggtttcacat ccggaataag gcttgagtca ctttcaacgc tctgatcata
    15481 aatcgcccga ccgatactgg aaacgtgacc ctgtaacgtt ttgttatcac tatagagggt
    15541 aatttgtgct ggtgcacctt cgcggatatg gcgcaacttg gtttcttcaa aataaccaat
    15601 gacgtaaaac gagtggctgt cgacaagggc aaacagcggt ttcccggtgt cagcgtaatc
    15661 gccgatgcgg gtagtgagat tagtcaccca tccactcacc ggggcgcgaa tttccgtttg
    15721 cgccagttgc cattgtgtct gtttcagcgt cgcctgtgcg gcatcaacac tggcctgcat
    15781 cgctttaaca ttgaggttag cggtatcgag ttcttcggca gaaataaaat tttgtgagag
    15841 atgacgacga cgattcgctt cgttattggc ttttgcgagg tcagattgcg ctttcgccag
    15901 ctgagcctgt gcattcagct cggcgatctg aaagggcgtt ttatcgatcg tgagcagaag
    15961 atcccccgcg ttaaccagct ggttgtcttt tatattcagc tcaacaatac gtcccgacac
    16021 ctgtggggtg atagaaacct gttctgcgcg tattttcccg tcgcgtgtcc acggagattg
    16081 catgtaataa ttccacagcc accatccggc aagaaccgca actaccgcta caatgattgt
    16141 tgagaaatac ttaattgttt taattgacat agttaccacg caatcagtat tgccagagca
    16201 aggcaaacgc aaatcgcaaa cagcgataaa tccattaaca agggatgcca gatgtcaccg
    16261 gcgtagatcc agccacgaag caggcggtgt acgacaagcc agatgacgaa tccaaacgcg
    16321 aaggctttga aaaacggagg aaagtagacg gacgcaccga acaccagatc ttgtaagggc
    16381 aatcctgttg cattgagcat aaacttcaca aagtagattc ctttacgacc gaatatattg
    16441 cgttagatta ataaatattc tttaactgcg aaaaatttac gcgcaatttc tgaaaaatat
    16501 gtctgacggt aaccaaatgc agcaatacat ttgttttagc aatacaattg ctgcacacta
    16561 ttctaaaagc cgcataatat cttagcaagc taattataag gagatgaaat tggaatcgcc
    16621 actaggttct gatctggcac ggttggtgcg catatggcgt gctctgatag accatcgcct
    16681 gaaaccgctg gagttaacac aaacccattg ggttacgtta cacaatatcc atcagttacc
    16741 tccagaccag tcgcaaattc aactggcaaa agcgattggc atcgagcagc catcactggt
    16801 ccgtactctg gaccaactgg aagaaaaagg gttaatttcg cgtcaaactt gtgccagcga
    16861 tcgtcgggct aaacgtatta aactgacgga aaaggcagag ccgctgatca gcgaaatgga
    16921 agctgttatt aacaaaaccc gcgcggaaat tttacatggc atctccgcag aggaactgga
    16981 acaactgatt acgctcatcg caaaacttga gcataatatc attgagttac aggccaaagg
    17041 gtgaaatgaa gggggcgtgt ggtcagtaac ctgaccacac gcaaacttat ttagcgcgga
    17101 gaaacggtca cctgactgcc attgctggcc agtacgacac gttggcccgg agagaaacga
    17161 gtgttgcctt gtttctgtac caccatgatg gtattaccat cgtctttacg aatttccagc
    17221 tcgacacctt gcgttttgtt cattgcactc tgtacgccct gaccagctac gccacctgca
    17281 acagcgcctg ctgcagtagc cagagaacgc ccggttccgc caccaacagt atttcccagg
    17341 aaaccaccaa gaacagcacc gccaattgca ccgataacgt tggaatcatc accgccctga
    17401 atctgtaccg gacgtacgtt aacgatggtt ccatagctga cattctgtac ttgtttcgct
    17461 tcagaagcgg tataaacatc ccctgacagg gtgtcgttat taacacaacc gacaagagac
    17521 agacctacca ttgaaacaac caatacgcgt ttaatcattg aaaaatctcc tgttcaccat
    17581 gaaacgccac gcaagcatcc ctcatggtca aagtatatgg catatgtgaa tccatggtta
    17641 catgttctac caaatatgag caaatcataa tgaaagtagg atcaaccaat tataaacaaa
    17701 acattcatga tgattattga tttgaagata atgttttact accaacggta aaaatcggtc
    17761 attccgcatg gtattcacga aaagtttatg tttgagtgtg cgcaaacgtc agattgaaaa
    17821 aggatgaact atgaaatcgg gccgctttat tggcgttatg tcaggcacca gccttgatgg
    17881 tgttgatgtt gtgttggcga caattgatga acaccgggtc gcacagctgg caagtttgag
    17941 ctggccgatc ccggtgtctc tgaaacaggc tgtactggat atttgccagg gccagcagct
    18001 tacactttcg cagtttggac agcttgatac tcaactcggg cgactttttg ctgatgcggt
    18061 caaggccttg cttaaggaac aaaacctgca ggcgagagat atagttgcga tcggctgtca
    18121 cggtcaaacc gtctggcatg aaccgacggg cgtggcacca cacactttac agattggcga
    18181 taacaatcaa attgtggcac gcaccggaat tacggttgtc ggtgattttc gccgtcgcga
    18241 tattgccttg ggaggacaag gcgcaccgct ggtacctgcg ttccatcatg ccttgctggc
    18301 tcacccaacc gagcgacgaa tggtgctcaa tattggcggc atcgccaatc tgtcactgct
    18361 cattcctggg cagccggttg gcggctacga taccggtcct ggtaacatgc tgatggatgc
    18421 ctggatctgg cgtcaggccg gtaaacctta cgataaagat gccgagtggg cacgggcggg
    18481 taaagttatt ctcccactgc tgcaaaatat gctcagcgac ccatatttct cgcaacctgc
    18541 accgaaaagc accggacgcg aatactttaa ctacggctgg ctggagcgcc atttacgcca
    18601 ttttccgggt gttgatcccc gtgatgttca ggccacactg gcagaactca ccgccgtgac
    18661 catttctgaa caagttttgt tgagcggtgg ctgcgaacga ttgatggtat gtggtggagg
    18721 tagtcgtaat ccgctactca tggcgcgtct ggcggcatta ctgccaggca cagaagtcac
    18781 caccaccgat gccgttggca ttagtggcga tgacatggaa gcattggctt tcgcttggct
    18841 tgcctggcgg acgctggcgg gattaccagg aaatctgcct tccgtcactg gcgcaagcca
    18901 ggagacggtt ctgggggcta ttttccccgc caactcgtga tatcagaatt aactgaattt
    18961 tttcttatcg tgtctgccgt tacacttgga agattaagga ggatatttgc cctccggacc
    19021 aggaaagtct tcgggatatg actatgaaaa aactgttaat tatcattttg ccggtccttc
    19081 tctccggctg tagcgccttt aatcaactgg ttgagcgaat gcaaaccgat acgctggaat
    19141 accagtgtga tgaaaaaccg ttgacggtca aactgaataa tccgcgccag gaagtcagtt
    19201 ttgtttacga taatcaacta ctgcatctca aacagggcct ttcagcctct ggcgcgcgtt
    19261 actccgacgg gatctatgtt ttctggtcga aaggtgagga agcgactgtc tataaacgcg
    19321 accgcatcgt cttgaataac tgtcagttac aaaatccaca gcgttgagat ttttccaggg
    19381 gcggcgcaca atagcgtcac ccacagacaa tccgtaaaga aaaccatgtc tgataacgac
    19441 gaattgcagc aaatcgcgca tctgcgccgt gaatacacca aaggcgggtt acgccgccgc
    19501 gatcttcccg ccgatccatt aacccttttt gaacgttggc tctctcaggc ttgtgaagcc
    19561 aaactggcgg accctaccgc gatggtggtc gctaccgtgg atgaacatgg tcagccttat
    19621 cagcgcatcg ttttactcaa acattacgac gaaaaaggca tggtgtttta caccaacctc
    19681 ggtagccgta aagcacatca aattgaaaat aatccgcgcg ttagcctgct gttcccgtgg
    19741 catacccttg aacgccaggt gatggtgatc ggtaaagcag aacgactttc gactctcgaa
    19801 gtgatgaaat attttcatag ccgcccgcgt gatagccaga ttggttcatg ggtttcgaag
    19861 cagtccagtc gcatttctgc ccgcggtatc cttgaaagta aattcctgga gctgaagcag
    19921 aagtttcaac aaggcgaagt gccattgccg agcttttggg gcggttttcg cgtcagcctt
    19981 gaacagattg agttctggca gggcggtgag catcgcctgc atgaccgctt tttgtaccag
    20041 cgtgaaaatg atgcgtggaa gattgatcgt cttgcaccct gaaaagatgc aaaaatcttg
    20101 ctttaatcgc tggtactcct gattctggca ctttattcta tgtctctttc gcatctggcg
    20161 aaaagtcgtg taccggcaaa ggtgcagtcg ttatatacat ggagattttg atggcaagca
    20221 gtaacttgat taaacaattg caagagcggg ggctggtagc ccaggtgacg gacgaggaag
    20281 cgttagcaga gcgactggcg caaggcccga tcgcgctcta ttgcggcttc gatcctaccg
    20341 ctgacagctt gcatttgggg catcttgttc cattgttatg cctgaaacgc ttccagcagg
    20401 cgggccacaa gccggttgcg ctggtaggcg gcgcgacggg tctgattggc gacccgagct
    20461 tcaaagctgc cgagcgtaag ctgaacaccg aagaaactgt tcaggagtgg gtggacaaaa
    20521 tccgcaagca ggttgccccg ttcctcgatt tcgactgtgg agaaaactct gctatcgcgg
    20581 cgaacaacta tgactggttc ggcaatatga atgtgctgac cttcctgcgc gatattggca
    20641 aacacttctc cgttaaccag atgatcaaca aagaagcggt taagcagcgt ctcaaccgtg
    20701 aagatcaggg gatttcgttc actgagtttt cctacaacct gttgcagggt tatgacttcg
    20761 cctgtctgaa caaacagtac ggtgtggtgc tgcaaattgg tggttctgat cagtggggta
    20821 acatcacttc tggtatcgac ctgacccgtc gtctgcatca gaatcaggtg tttggcctga
    20881 ccgttccgct gatcaccaaa gcagatggca ccaaatttgg taaaactgaa ggcggcgcag
    20941 tctggttgga tccgaagaaa accagcccgt acaaattcta ccagttctgg atcaacactg
    21001 cggatgccga cgtttaccgc ttcctgaagt tcttcacctt tatgagcatt gaagagatca
    21061 acgccctgga agaagaagat aaaaacagcg gtaaagcacc gcgcgcccag tatgtactgg
    21121 cggagcaggt gactcgtctg gttcacggtg aagaaggttt acaggcggca aaacgtatta
    21181 ccgaatgcct gttcagcggt tctttgagtg cgctgagtga agcggacttc gaacagctgg
    21241 cgcaggacgg cgtaccgatg gttgagatgg aaaagggcgc agacctgatg caggcgctgg
    21301 tcgattctga actgcaacct tcccgtggtc aggcgcgtaa aactatcgcc tccaatgcca
    21361 tcaccattaa cggtgaaaaa cagtccgatc ctgaatactt ctttaaagaa gaagatcgtc
    21421 tgtttggtcg ttttacctta ctgcgtcgcg gcaaaaagaa ttactgtctg atttgctgga
    21481 aataatgcat taagtggaaa gggggagtga gaaatcactc cccctggttt ttatacaggg
    21541 aacatgatga aaaatattct cgctatccag tctcacgttg tttatggtca tgcgggtaac
    21601 agtgcggcag agtttccgat gcgccgcctg ggcgcgaacg tctggccgct gaacaccgtt
    21661 caattttcta atcacaccca atacggcaaa tggactggct gcgtgatgcc gcccagccat
    21721 ttaaccgaaa ttgtgcaagg cattgccgcc attgataaat tacacacctg tgatgccgta
    21781 ttaagtggct atctgggatc ggcggagcag ggtgaacata tcctcggtat cgtccgtcag
    21841 gtgaaagcag cgaatccgca ggcgaaatat ttttgcgatc cggtaatggg tcatccagaa
    21901 aaaggctgta tcgttgcacc gggtgtcgca gagtttcatg tgcggcacgg tttgcctgcc
    21961 agcgatatca ttgcgccgaa tcttgttgag ctggaaatac tctgtgagca tccggtaaat
    22021 aacgtcgaag aagcggttct ggcagcgcgc gaactcattg cgcaagggcc acaaattgta
    22081 ttggttaaac acctggcgcg agctggctac agccgtgacc gttttgaaat gttgctggtc
    22141 accgccgatg aagcctggca tatcagccgt ccactggtgg attttggtat gcgccagccg
    22201 gtaggtgttg gtgatgtgac gagcggttta ctgctggtga aactgcttca gggggcaacg
    22261 ctgcaggagg ccctggaaca tgtgaccgct gcagtctacg aaatcatggt gaccaccaaa
    22321 gcaatgcagg aatatgagtt gcaagtggtt gctgctcagg atcgtattgc caaaccagaa
    22381 cattacttca gcgcaacaaa gctctgaaat atctcatttc ggcccacaac gaactgtggg
    22441 ccgacggcta ttactttaag ccttccgctg acagcgcgtc ttgtacttcc ggacgttcag
    22501 ccatacgttg cataaatgcc gcaatgtgct ctaacccttc cagattcagt ttcaccgcgt
    22561 atgcccagcg cagtaccgta aacagatagg catcagcaat tgtaaatctt tgcccgcaga
    22621 tccagtgctc atccttcagt gcctcgttca catattgcag tttcttctcc agctgcgcgc
    22681 gaactgtcgg tttgtactct tccggtgtat cagggcgaaa cagaggtgtg aaacctttat
    22741 gcagctcggt ggcgatgtaa ttcagccatt cgatggtttt atagcgggaa atactgttta
    22801 ccggtgccag caactggcgg tcggggacgc tgtcggcaag atactgcata atcgctacac
    22861 cttccgtcag caaagtaccg tcatccagca gcaatgcagg tacctgtccc ttagggttaa
    22921 cggcaaagta atcgtcaccg ttttcgagac gttttttcat taaatccaca ctgacgagcg
    22981 taaaatcctt tccgctctca cgcagggtga tatgggaagc gagagagcag gcgcccggtt
    23041 tgtagaacaa tttcatcggt aactcctttt gtctgtggtt taaagtatgg tagtgcgttg
    23101 ctgaacaaaa aaaaagccgc taacgaaaag ttagcggctt gtaaaagagt ttccctgaat
    23161 tttacgctac ggctgctttc gccgctttgt ctgcagcgtc atcctgcgtc atgcggtgca
    23221 gtttcggcgc ggtcagcagc atcagtactg caataacggc agtagcgaca ccaatctgca
    23281 agaatacgcg accatagact tccagtgaca tcagcggatc ggtaacgtta tccggcacag
    23341 ccatcatacc cgcaacataa ccaccaatca ggtttgcacc ggcagtggtc aggaaccagc
    23401 tacccataat gaagcccatc agacgctgcg gaacgagttg tgcaaccatt gccagaccca
    23461 gaccagagat catcagttcc ccgatactct gcaggccata gctcgcgacc agccagctta
    23521 cagacacgat acctgcgtca gacgcgaatt tcgcacccag cggcagaatc aggaacgcac
    23581 cagaacacat caccataccg attgcaaact tggttggcat cggcagggta tcgcccatct
    23641 tgttatagat agcggccaga atcgggctac caatgatgat ccagaacggg ttcagtgcct
    23701 gatactgctc aggttctacg gccaggccca gaatggtatg ttcaacgtta cgaatcgcaa
    23761 agaagttcag tgacgttggc atctggctgt acagcacgaa gaagataatg gcttcgagca
    23821 tcaggatgaa ggcaacgatc attttacgac gcgcagcacc tttcatcgcg aaggcttctt
    23881 taccgaagat aaccacgata ccgaaggcaa caacgcccag cgccatacgc gcaacttcct
    23941 gattgtgcag cagccaggtg gcgatagcga tcagtgccac aacaccaata atggtcagca
    24001 gcaggttacg gtagttgata ggctcgaagt ctggttttga accgttctgt ttaacccagc
    24061 gttggcagaa ggcgaagtta acgatagtga tcagcaggcc tacaacgctc aacgcaaacg
    24121 caacactcca gccgtatttc gcggccagcc acggggtggc gatcatagag aagaaagagc
    24181 cgatgttgac ggacatgtag tacatggtga atgcaccgtc cagacgcggg tcgtttttct
    24241 catagcatgt agaaagcaga gaagacgggt tagctttaaa caggccgtta ccgaccgcaa
    24301 tagccgccat acccatataa acgataccgg cgtcgtgacc agaccaggca accagagcat
    24361 aaccaatcgc cagcacaata gcgccgagca taattacgcg tttagtaccc agtaccttgt
    24421 cacctaacca gccgccgata gcgaccagac cataaaccag ggcactaaag gaagagaaaa
    24481 gggtgattga atccgcttca gacataccca gttgtttaac caggtaaaca gccataattc
    24541 cttgtaggcc gtaataacca aaacgttccc ataactcaat cgagaagatg agatagaacg
    24601 ccttcggttg tttgaaagcg ttcaaactga cgctttcagt tggtttttgg tttgcagtgg
    24661 acacttttac ctcttttttt acatcccata ttaacggggg tgctttctcg cgttatgtct
    24721 aaaaaacata cgcgcaattg ttatagtggg aggggaaacg gcaggtaatg ttcactatct
    24781 tgcactttca gacaagtcct ttgtaataat ctgttacata taactgaggc ggggaattca
    24841 accattccgg gaaatgttat ccagcgttaa atttttcaaa aaacgtaacg gcagattttt
    24901 tcactgcatt gagaggg
//
LOCUS       DBFIFM010000053        24660 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.053, whole genome shotgun sequence.
ACCESSION   DBFIFM010000053 DBFIFM010000000
VERSION     DBFIFM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.053"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(113..2686)
                     /gene="clpB"
                     /locus_tag="N7C74_001866"
     CDS             complement(113..2686)
                     /gene="clpB"
                     /locus_tag="N7C74_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HGU2431745.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2816..3547)
                     /gene="yfiH"
                     /locus_tag="N7C74_001867"
     CDS             complement(2816..3547)
                     /gene="yfiH"
                     /locus_tag="N7C74_001867"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HGU2431746.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLNLTGEPYASKRADASYSNTPGRVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAEASTAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3544..4524)
                     /gene="rluD"
                     /locus_tag="N7C74_001868"
     CDS             complement(3544..4524)
                     /gene="rluD"
                     /locus_tag="N7C74_001868"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HGU2431747.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKLCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4659..5396
                     /gene="bamD"
                     /locus_tag="N7C74_001869"
     CDS             4659..5396
                     /gene="bamD"
                     /locus_tag="N7C74_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HGU2431748.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5667..6008
                     /gene="raiA"
                     /locus_tag="N7C74_001870"
     CDS             5667..6008
                     /gene="raiA"
                     /locus_tag="N7C74_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HGU2431749.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6111..6236
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6112..6159
                     /gene="pheL"
                     /locus_tag="N7C74_001871"
     CDS             6112..6159
                     /gene="pheL"
                     /locus_tag="N7C74_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HGU2431750.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6258..7418
                     /gene="pheA"
                     /locus_tag="N7C74_001872"
     CDS             6258..7418
                     /gene="pheA"
                     /locus_tag="N7C74_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HGU2431751.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7461..8582)
                     /gene="tyrA"
                     /locus_tag="N7C74_001873"
     CDS             complement(7461..8582)
                     /gene="tyrA"
                     /locus_tag="N7C74_001873"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HGU2431752.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPALRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8593..9663)
                     /gene="aroF"
                     /locus_tag="N7C74_001874"
     CDS             complement(8593..9663)
                     /gene="aroF"
                     /locus_tag="N7C74_001874"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HGU2431753.1"
                     /translation="MQKDALNNVHITDEQVLMTPAQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9873..10238
                     /gene="yfiL"
                     /locus_tag="N7C74_001875"
     CDS             9873..10238
                     /gene="yfiL"
                     /locus_tag="N7C74_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HGU2431754.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASAIRLN"
     gene            10388..10906
                     /gene="yfiR"
                     /locus_tag="N7C74_001876"
     CDS             10388..10906
                     /gene="yfiR"
                     /locus_tag="N7C74_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HGU2431755.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            10896..12122
                     /gene="dgcN"
                     /locus_tag="N7C74_001877"
     CDS             10896..12122
                     /gene="dgcN"
                     /locus_tag="N7C74_001877"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HGU2431756.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLP
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSAA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYGVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIAHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12138..12620
                     /gene="yfiB"
                     /locus_tag="N7C74_001878"
     CDS             12138..12620
                     /gene="yfiB"
                     /locus_tag="N7C74_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HGU2431757.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITPP
                     "
     gene            complement(12697..13044)
                     /gene="rplS"
                     /locus_tag="N7C74_001879"
     CDS             complement(12697..13044)
                     /gene="rplS"
                     /locus_tag="N7C74_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HGU2431758.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13086..13853)
                     /gene="trmD"
                     /locus_tag="N7C74_001880"
     CDS             complement(13086..13853)
                     /gene="trmD"
                     /locus_tag="N7C74_001880"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HGU2431759.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(13884..14432)
                     /gene="rimM"
                     /locus_tag="N7C74_001881"
     CDS             complement(13884..14432)
                     /gene="rimM"
                     /locus_tag="N7C74_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HGU2431760.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14451..14699)
                     /gene="rpsP"
                     /locus_tag="N7C74_001882"
     CDS             complement(14451..14699)
                     /gene="rpsP"
                     /locus_tag="N7C74_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HGU2431761.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(14836..16197)
                     /gene="ffh"
                     /locus_tag="N7C74_001883"
     CDS             complement(14836..16197)
                     /gene="ffh"
                     /locus_tag="N7C74_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HGU2431762.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16364..17155
                     /gene="ypjD"
                     /locus_tag="N7C74_001884"
     CDS             16364..17155
                     /gene="ypjD"
                     /locus_tag="N7C74_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HGU2431763.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17177..18462
                     /gene="yfjD"
                     /locus_tag="N7C74_001885"
                     /pseudo
     CDS             17177..18462
                     /gene="yfjD"
                     /locus_tag="N7C74_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
     gene            complement(18517..19110)
                     /gene="grpE"
                     /locus_tag="N7C74_001886"
     CDS             complement(18517..19110)
                     /gene="grpE"
                     /locus_tag="N7C74_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HGU2431764.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKVKA"
     gene            19233..20111
                     /gene="nadK"
                     /locus_tag="N7C74_001887"
     CDS             19233..20111
                     /gene="nadK"
                     /locus_tag="N7C74_001887"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HGU2431765.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGATRTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20197..21858
                     /gene="recN"
                     /locus_tag="N7C74_001888"
     CDS             20197..21858
                     /gene="recN"
                     /locus_tag="N7C74_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HGU2431766.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IVEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPETLPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKTRLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22007..22348
                     /gene="bamE"
                     /locus_tag="N7C74_001889"
     CDS             22007..22348
                     /gene="bamE"
                     /locus_tag="N7C74_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HGU2431767.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22410..22700)
                     /gene="yfjF"
                     /locus_tag="N7C74_001890"
     CDS             complement(22410..22700)
                     /gene="yfjF"
                     /locus_tag="N7C74_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HGU2431768.1"
                     /translation="MPGKIAVEVAYALPKKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTENKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22690..23127)
                     /gene="ratA"
                     /locus_tag="N7C74_001891"
     CDS             complement(22690..23127)
                     /gene="ratA"
                     /locus_tag="N7C74_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HGU2431769.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            23298..23780
                     /gene="smpB"
                     /locus_tag="N7C74_001892"
     CDS             23298..23780
                     /gene="smpB"
                     /locus_tag="N7C74_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HGU2431770.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            23995..24357
                     /gene="ssrA"
                     /locus_tag="N7C74_001893"
     tmRNA           23995..24357
                     /gene="ssrA"
                     /locus_tag="N7C74_001893"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         6292 a         6501 c         5777 g         6090 t
ORIGIN      
        1 gaaaaatata aaaactttat cgttcgctca tttttcaggc aaaacatctt aaatatagtc
       61 ttttccgtct aacttataga caaaaacgag ccccgaaggg ctcgttttat cattactgga
      121 cggcgacaat ccggtcttca ttaacttcca ggcgaataac tttacccgga accaattcac
      181 cagacagtat ttgctgtgcc aacgggtttt cgatctgctg ctggatagca cgtttcaaag
      241 gacgtgcacc atagactgga tcgtaaccgt tctcgctcag cagtttcagc gcctcgtcag
      301 aaatgtggat ttcataacca cgttcttcca gacgtttgta cagacgtttc aactgaatct
      361 gcgcaatcga ggcaatgtgc tgttcgccca gcggatggaa gaccaccact tcatcgatac
      421 ggttaatgaa ctccggacgg aagttatggc ttaccacacc gagcaccagc tctttcatgt
      481 gcgcataatc cagttcaccg aagcgttcct gaatcagatc ggaaccgagg ttagaggtca
      541 taatgacgac cgtattacgg aagtcgaccg ttctcccttg cccgtcagtc agacgcccat
      601 catccagtac ctgcaacaga atgttgaaga catccggatg cgctttttcc acttcatcca
      661 gcaggatgac ggaatacgga cgacgacgca ccgcttcggt caggtagcca ccttcttcat
      721 aaccgacata tcccggaggc gcaccaacca gacgagacac cgagtgtttc tccataaact
      781 cggacatatc gatacggacc atcgcctcgt cgctatcaaa cataaagttc gccagcgcct
      841 tacaaagctc tgttttcccc acaccagttg ggccgaggaa caggaatgaa ccaatcgggc
      901 gatttggatc cgccagcccc gcacggctac gacgaatagc gttagatacc gcatcaaccg
      961 cttcgttctg accaattacg cgatggtgca gttcttgctc catacgcagc agtttttcgc
     1021 gctcgctttc catcatgcga gaaaccggaa tccccgtcca acgcgccagc acttcagcaa
     1081 tttcggcgtc ggtcacttta ttacgcaaca gacgcatagt tttgccttcg agctgcgttg
     1141 cggcttccag ttgcttttcc agttccggga ttttgccgta ttgcagttca gacatccgcg
     1201 ccaagtcacc cacacggcga gcctgttcaa tagcgatttt cgcctgttcc agttccgctt
     1261 taatggtctg cgtaccagaa agcgatgcct tctctgcttt ccactcttct tctaactcgg
     1321 agtactgacg ttctttgtcg ctcagttctt cgttgagcat atccagacgt tttttactgg
     1381 cttcatcaga ctctttcatt aacgcctgtt gttccagttt gagctggatg atacggcgat
     1441 cgagtcggtc gagttcttct ggttttgagt caatctgcat acgaatgctg gatgctgctt
     1501 catcgatcag gtcgatggct ttatccggca gctgacggtc agcaatgtag cgatgagaca
     1561 acgtcgccgc tgcaacaatt gccgggtcag taatttgcac atggtggtgc aattcgtaac
     1621 gttctttcag gccacgcaga atcgcaatgg tatcttcaac agaaggctcg gcaacaaaca
     1681 ctttctggaa acgacgttcc agcgcagcat ctttttcgat gtactggcga tattcgtcaa
     1741 gcgtcgtggc acctacgcag tgcaattcac cacgcgccag cgccggtttc agcatgtttc
     1801 cggcgtccat tgcgccatcg gctttacccg cgccgaccat ggtatgtaat tcgtcgataa
     1861 acaggatgac gttgccttcc tgtttggcaa gatcgttaag cacgcctttt aaacgttctt
     1921 caaactcacc gcgatatttc gccccagcca ccagcgcgcc catatccagc gccagtaccc
     1981 ggcggccttt caacccttcc ggcacttcgc cgttgataat acgctgcgcc agaccttcaa
     2041 cgatggcagt tttaccgacg ccgggttcac caatcagtac cgggttattt ttagtacgac
     2101 gttgcagcac ctgaatggta cggcgaattt cttcatcacg accaatcacc ggatcgagtt
     2161 tgccctgttc ggctcgttcg gtaaggtcga tggtatattt tttcaaagcc tgacgttggt
     2221 cttcagcacc ttgatcgttc acgctttcac ctccacgcat ttgttcaatc gcttgagtaa
     2281 tgttggcggt ggtcgcccct gctgctttca ggatgtcggc cagcgtgccg cgagactcaa
     2341 gtgccgccag aacgaacagt tctgacgaga taaagttatc accacgtttt tgcgccagct
     2401 tgtcgcaaag attaagaacg cgcaccagat cctgtgatgg ctggacatca ccaccagtac
     2461 cttcaacctg cggtaaacga tttaatgcct gattgatatc tgtgcgcaac tggccagcat
     2521 ttatgccagc ggatgttaat aaaggactaa ccgaaccccc ttcctgattc agcagggcgc
     2581 tcattaaatg aagtggttcg ataaattggt tgtcgtgccc aagtgcaagt gattgggcat
     2641 cggcaagagc aagctggaat ttattagtaa gacgatccag acgcataact cctcccataa
     2701 cggatcaaag ttgctactgg agattaaatg aggtcatccc tcaattattc aaggttattg
     2761 accagattaa tgtgaaaaga aaatcacgcg taccggatcg tcttgattct ttaggttata
     2821 tcagccaaat gaaacttgcc atacgaccgg tggtcttgtc gcgacgataa gagaagaaag
     2881 tctcattttc cgtatatgta caacggtcgc cgccgaaaat ttgctcaaca cccacgttcg
     2941 ccagacgctg ccgggcaagc tgataaatgt ccgccagata cttatcacca tgctgaatga
     3001 aagctgtact cgcttcggcg tctactgcca taaacgcctc gcgaacctcc gcccccactt
     3061 caaacgcgcg tgggccaatt gccggcccta accaggcgag aatattttct ggattatcag
     3121 caaaacagga aactgtctct tccagcacgc ctgcgcacag tccacgccag ccagcatgag
     3181 cggcggcgac ttccgttccc gctcgattgc aaaacagcac agggaggcag tcggcagtca
     3241 tcactgcgca aaccctgccg ggcgtattgc tataagaggc atctgctcgt tttgaggcat
     3301 aaggttcgcc agtaagatta agcacatctt tgccgtgtac ctgctcaagc cagaccggtt
     3361 tagaaggcaa attgcccgca gcaaaaagtc gcttgcgatt ctcctcaacg tgatccgggt
     3421 tatcgccaca atgggcaccg aggttgagcg agtcatacgg gggcaagctc acgccgccga
     3481 tacgagtaga gctacaggcc gcaacacctt ttggcagcgg ccactgcggg acaatcagct
     3541 tactcataac cagtccactt catctttatg ctcttcgaaa tcggcgcgca tcacctcaat
     3601 cagctccacc atatcttgtg gaataggcgc atgccattcc atttcgatac cggagatcgg
     3661 gtgataaaga cgcagcatgg ttgcatgaag cgcctggcgg tcaaatttac gcagcgtgga
     3721 gataaacgct tccgaagcac cttttggcgg gcgcggacgg ccaccataaa ccggatcgcc
     3781 caccagcgga tgagtgatat gggccatatg cacgcggatc tggtgcgtac gtccagtttc
     3841 cagacgcaac cgcagacgcg tgtgcacacg gaagtgttcc atgatgcgat agtgagtcac
     3901 cgccggtttg cccatcggat gcaccgccat gtgagtacgt ttggtcgggt ggcggctgat
     3961 tggttcatct actgtaccgc cagcggtcat atgaccaatt gccaccgctt catactcacg
     4021 agtaatttca cgcctttgca aagattcgac taaacgcgtc tgagccggaa cggtttttgc
     4081 cacaaccatc aggccagtgg tgtctttatc cagacgatgg acgatgcctg cacgcggtac
     4141 atcagcaatg ggcggatagt aatgaagtaa cgcattcagt accgtgccat ccgggttacc
     4201 cgcgccagga tgtaccacca gatcgcgcgg tttattaatg acgatgatat cttcatcttc
     4261 atagacgata tccagcggga tatcctgcgg ttcaaaacgc gcttcttctt caatctcagc
     4321 gttgatggca acctgctcgc cacccaatac tttttctttc ggcttatcac aaagtttgcc
     4381 gttaaccagc acgcgctggt cgaggatcca ttcttttatt cgcgaacgtg aataatccgg
     4441 gaacatttcg gccaaagcct gatctaagcg ttgaccgagt tggttttcgg acaccgttgc
     4501 agtgagctgt actcgttgtg ccatatatac tgcttcttcg tttatcgttg gggttttacg
     4561 gctttgccgt ttaatatagt gtgctattgt agctggtctt aaccgggagc aggaacagag
     4621 aatctcccgt attacatttt gaggaaagtc aaaacgtcat gacgcgcatg aaatatctgg
     4681 tggcagccgc cacactaagc ctgtttttgg cgggttgctc ggggtcaaag gaagaagtac
     4741 ctgataatcc gccaaatgaa atttacgcga ctgcacaaca aaagctgcag gacggtaact
     4801 ggagacaggc aataacgcaa ctggaagcgt tagataatcg ctatccgttt ggtccgtatt
     4861 cgcagcaggt gcagctggat ctcatctacg cctactataa aaacgccgat ttgccgttag
     4921 cgcaggctgc catcgatcgt tttattcgcc ttaacccgac ccatccgaat atcgattatg
     4981 tcatgtacat gcgtggcctg accaatatgg cgctggatga cagtgcgctg caagggttct
     5041 ttggcgttga ccgtagcgat cgcgatcctc aacatgcacg agctgcgttt agtgactttt
     5101 ccaaactggt gcgcggctat ccaaacagtc agtacaccac cgatgccacc aaacgtctgg
     5161 tattcctgaa agatcgtctg gcgaaatatg aatactccgt ggccgagtac tatacagaac
     5221 gtggcgcatg ggttgccgtc gttaaccgcg tagaaggcat gttgcgcgac tacccggata
     5281 cccaggctac gcgtgatgcg ctgccgctga tggaaaatgc ataccgtcag atgcagatga
     5341 atgcgcaagc tgaaaaagta gcgaaaatca tcgccgcaaa cagcagcaat acataacaga
     5401 aacctgaaac acaaaacggc agcccttgag ctgccgtttt tttattctgt cagttgtgaa
     5461 actgaagcga tttagtcact atcgatctca tcaaatatgg ctcgctttga gatattcctc
     5521 aagtaaaaaa acacctcttc ctgcgatttc tcacaaaaaa gattcgttga caaaaagtga
     5581 caaaattatg agatttccat cacacatttt gacatcagga acggtatgct gaattcacca
     5641 agacgggaag acaagaggta aaatttatga caatgaacat taccagcaaa caaatggaaa
     5701 ttactccggc catccgccaa catgtcgcag accgtctcgc caaactggaa aaatggcaaa
     5761 cacatctgat taatccacat atcattctgt ccaaagagcc acaagggttt gttgctgacg
     5821 ccacaatcaa tacacctaac ggcgttctgg ttgccagtgg taaacatgaa gatatgtaca
     5881 ccgcaattaa cgaattgatc aacaagctgg aacggcagct caataaactg cagcacaaag
     5941 gcgaagcacg tcgtgccgca acatcggtga aagacgccaa cttcgtcgaa gaagttgaag
     6001 aagagtagtc ctttatattg agtgtatcgc caacgcgcct tcgggcgcgt tttttgttga
     6061 cagcgtgaaa acagtacggg tactgtacta aagtcactta aggaaacaaa catgaaacac
     6121 acaccgtttt tcttcgcatt cttttttacc ttcccctgaa tgggaggcgt ttcgtcgtgt
     6181 gaaacagaat gcgaagaaga acaataaggc ctcccaaatc ggggggcctt ttttattgat
     6241 aacaaaaagg caacactatg acatcggaaa acccgttact ggcgctgcga gagaaaatca
     6301 gcgcgctgga tgaaaaatta ttagcattac tggcagagcg gcgcgaactg gccgtcgagg
     6361 tgggaaaagc caaactgctc tcgcatcgcc cggtacgtga tattgatcgt gaacgcgatt
     6421 tactggaaag attaattacg ctcggtaaag cgcaccatct ggacgcccat tacattactc
     6481 gcctgttcca gctcatcatt gaagattccg tattaactca gcaggctttg ctccaacaac
     6541 atctcaataa aattaatccg cactcagcac gcatcgcttt tctcggcccc aaaggttctt
     6601 attcccatct tgcggcgcgc cagtatgctg cccgtcactt tgagcaattc attgaaagtg
     6661 gctgcgccaa atttgccgat atttttaatc aggtggaaac cggccaggcc gactatgccg
     6721 tcgtaccgat tgaaaatacc agctccggtg ccataaatga cgtttacgat ctgctgcaac
     6781 ataccagctt gtcgattgtt ggcgagatga cgttaactat cgaccattgt ttgttagtct
     6841 ccggcacgac tgatttatcc accatcaata cggtctacag ccatccgcag ccattccagc
     6901 aatgcagcaa attccttaat cgttatccgc actggaagat tgaatatacc gaaagtacgt
     6961 ctgcggcaat ggaaaaggtt gcacaggcaa aatcaccgca tgttgctgcg ttaggaagcg
     7021 aagctggcgg cactttgtac ggtttgcagg tactggagcg gattgaagcg aatcagcgac
     7081 aaaacttcac ccgatttgtg gtgttggcgc gtaaagccat taacgtgtct gatcaggttc
     7141 cggcgaaaac gacgttgtta atggcgaccg ggcaacaagc cggtgcgctg gttgaagcgt
     7201 tgctggtact gcgcaaccac aatctgatta tgacccgtct ggaatcacgc ccgattcacg
     7261 gtaatccatg ggaagagatg ttttatctgg atattcaggc caatcttgaa tcagcggaaa
     7321 tgcaaaaagc attgaaagag ttaggggaaa tcacccgttc aatgaaggta ttgggctgtt
     7381 accctagtga gaacgtagtg cctgttgatc caacctgatg aaaaggtgcc ggatgatgtg
     7441 aatcatccgg cactggatta ttactggcga ttgtcattcg cctgacgcaa taacacgcgg
     7501 ctttcactct gaaaacgctg tgcgtaatcg ccgaaccagt gctccacctt gcggaaactg
     7561 tcaataaacg cctgcttatc gccctgctcc agcaactcaa tcgcctcgcc gaaacgctta
     7621 tagtaacgtt tgattaacgc cagattacgc tctgacgaca taataatgtc ggcataaagc
     7681 tgcggatcct gagcgaacag tcgcccgacc atcgccagct caaggcggta aatcggcgaa
     7741 gagagcgcca gaagttgctc aagctgaaca ttttcttctg ccaggtgcag cccgtaagca
     7801 aaagtagcaa agtggcgcag tgcctgaata aacgccatat tctgatcgtg ctcgacggcg
     7861 ctaatacgat gcaaccgagc gccccagacc tgaatttgct ccagaaacca ttggtatgct
     7921 tccggtttac gtccatcaca ccagaccaca acttgctttg ccaggctacc gctgtccgga
     7981 ccgaacattg ggtgtaaccc cagtaccggg ccatcgtgcg ccgccagcat ggcctgtaat
     8041 ggtccatttt tcactgatgc cagatcaacc agaatacaat ctttcggtaa aggcggtaat
     8101 ttgccaataa cttgctcagt aacgtggatt ggcacactaa caatcaccat tccggcatcg
     8161 gcaacaatat cagccgctcg atcccagtca tgttgctcca gaatccgcac ctgataaccc
     8221 gagagtgtca gcatcttctc gaacagacgt cccatctgac cgccgccgcc aacgataact
     8281 accgggcgta acgcaggaca aagcgtttta aatcctttgt cgttttcact ggagtaagat
     8341 tcacgcatca cccgacgcaa aacatcctca atcagatctg gcggtacacc cagagcttcc
     8401 gcctctgcac gacgcgaggc caacatagat gcctctcgct ccggaacata aataggcagt
     8461 ccaaagcggc ttttcacctc gcccacttca gcaaccagtt ccagacgctt cgctaataaa
     8521 ttcagcagcg ctttatcgac ttcatcaatt tgatcgcgta atgcggtcaa ttcagcaacc
     8581 ataataaacc tcttaagcca cgcgagccgt cagctgcccg ttcagatcct gatgaatttc
     8641 acgcagcaag gcatcggtca tttcccagct aatgcaggca tcggttacgg atacaccgta
     8701 tttcatttca ctgcgcggtt gctcggaaga ctgattgccc tcgtggatat tactttcgat
     8761 catcagacca ataattgagc gattgccatc tttgatttga gcaaccacgg attctgccac
     8821 cgcaggctga cggcgataat ctttattgga attaccgtgg ctgcaatcta ccatcagaga
     8881 cgggcgcagt cccgcctgtt ccatctcttt ttcacattgc gcaacatccg cagggctata
     8941 gttcggcgct ttaccaccgc gcaggatgac atgcccgtcc ggattcccct gagtttgtag
     9001 caacgcaacc tgccctgcct ggttaatgcc aacaaaacgg tgcggctggg cggcggcgcg
     9061 catagcgtta attgctgttg ccagactgcc gtcggtgccg tttttaaagc caaccggcat
     9121 ggaaagcccg gaggccattt cacggtgagt ttgcgattcc gttgtacgag caccaattgc
     9181 tgaccagcta aacagatcgc ccaggtattg cgggctattc ggatctaacg cttccgtcgc
     9241 cagtggcagt cccatattca ccagctcaag cagcaattta cgcgcgatct gcagcccggc
     9301 ttctacatca aaagagccat ccatatgggg atcgttaatt aaccctttcc agccgacagt
     9361 ggtacggggt ttttcaaaat agacgcgcat taccagatag aggctatcgc tgacctctgc
     9421 ggcaagggct ttaaatcgac gagcatattc cagagcagtt tccggatcat gaatggaaca
     9481 agggccacat actaccagca gacgaggatc gcgcccggcg ataatatctg aaatggtttt
     9541 acgcgagtca gcaatctggg cttcttgttg caggcttaat ggaaaagcgg ccttcagttg
     9601 tgccggagtc attaaaacct gttcgtcggt aatatgtacg ttattcagcg cgtctttttg
     9661 catgatggcg atcctgttta tgctcgtttg cgatagttga tcctcagcga ggatgacgta
     9721 acgataacac ataaagtaaa gttttcaatc catatttcgt acatttttct ttacataagc
     9781 aatttattcg cgctttcaac ccttgattct gtatagataa atttacacca cctttgaaaa
     9841 caatccgcta tgctttgaaa aaggagaaag aaatgatgaa aaagtttatc gcccccttgt
     9901 tggctttact ggttagcgga tgccagattg atccttatac tcacgcgcca accttgacca
     9961 gcaccgactg gtatgatgtt ggtatggaag atgcgatatc gggcagcgcc ataaaagatg
    10021 acgatgcatt tagcgattca caggcggatc gcggtctata ccttaaagga tatgccgaag
    10081 gacaaaagaa aacttgccag accgatttta cttatgcccg aggactttcc ggtaaaagct
    10141 ttcctgccag ctgcaacaat gttgaaaatg ccagccaact gcatgaagta tggcaaaaag
    10201 gggctgatga aaatgccagc gcaatacgat taaattaatt aaaaccccac aaataaatta
    10261 aacataagat tttactcatt atgttttatt tctcacaatg atgacgaccg catgaaataa
    10321 tgatatttta atgagcattc tgaaaaaata tcgatgcatt tcgagcgaag atggtgggga
    10381 tccctgaatg cgtttttctc accgactgtt cttactgctg attctcctgc tgacgggagc
    10441 accaatactc gctcaggagc cttctgacgt tgcgaaaaac gtgcgtatga tggtttccgg
    10501 tattgtcagt tacacccgct ggcctgcgct atcagggcca ccaaagttat gtatcttttc
    10561 atcttcacgt tttagtaccg ccctacaaga gaatgccgca acatctttgc cttatctgcc
    10621 tgtcatcatt cacactcaac aagaggcgat gatttcaggc tgcaatggtt tttattttgg
    10681 caacgagtca ccgacattcc agatggaatt aaccgaacaa tatccgtcaa aggcgttatt
    10741 attaattgcc gaacaaaata ccgagtgcat tattggtagc gccttttgtc tgatcatcca
    10801 caataatgac gtcagatttg ccgtaaacct ggatgcctta tcgcgtagcg gtgtaaaggt
    10861 caacccggat gtcttaatgc tcgcacggaa gaaaaatgat ggataacgat aattctctta
    10921 ataagcgccc cacgtttaaa agagcattac gcaacatcag tatgaccagc atatttatca
    10981 ctatgatgct gatctggttg ctgctttccg tgacctcggt gctgcccctg aaacagtacg
    11041 cgcaaaaaaa cctggcactg acagcagcaa caatgactta cagtctggaa gcagctgtcg
    11101 tttttgccga tggccctgca gcaactgaaa cactggcagc gctgggccag caagggcaat
    11161 tttcaactgc agaagtacgt gataagcagc aaaatattct ggcatcctgg cattacaccc
    11221 gtaaggatcc aggcgatact ttcagcaatt tcataagcca ctggctcttc cctgccccca
    11281 tcattcagcc gattcgtcac aatggtgaaa ccattggcga agtacgctta accgctcgcg
    11341 acagttcaat cagccatttt atctggtttt cgctcgccgt actgaccggt tgtattctgc
    11401 tggcatcagg aatcgcaatt accctcaccc gccatttgca caatggcctg gtggaagcac
    11461 tgaaaaatat caccgatgtc gtacatgatg tgcgttccaa ccgcaatttt tcccgacgag
    11521 tttcggaaga acgtatcgct gagtttcacc gcttcgctct cgacttcaac agtctgctgg
    11581 atgaaatgga agagtggcag cttcgtttac aggccaaaaa tgcgcagctt ctacgtaccg
    11641 cgctacatga cccattaacc gggctggcta accgcgcagc gtttcgtagc ggcatcaata
    11701 cgttgatgaa caattccgct gcccgaaaaa cgtcggcgtt actatttctt gatggcgata
    11761 atttcaaata tatcaatgat acctggggtc atgcgacggg cgatagagtc ttgattgaaa
    11821 tcgcaaaacg gttagctgaa tttggcgggc tgcgacataa agcataccgc ctgggcggcg
    11881 atgaattcgc tatggtgctc tatggtgtac agtcggaatc tgaagtgcag cagatatgct
    11941 cagcactgac acaaatcttt aatctcccgt ttgatcttca taatggccat cagaccacca
    12001 tgacattaag cattggttac gcaatgacca ttgcgcacgc ttctgcggaa aaattacaag
    12061 agctggccga tcacaatatg tatcaggcca aacaccagcg tgccgaaaag ctggtgagat
    12121 aacaaggata tatcgatatg ataaagcacc tggtagcacc cctgattttc acctcactaa
    12181 tactgactgg ctgccagtcc cctcagggaa agtttactcc tgagcaagtc gccgctatgc
    12241 aatcttatgg atttactgaa tccgccggcg actggtcgct gggcttatca gatgccattc
    12301 tgttcgcaaa aaatgactac aaattgctcc cggaaagcca gcaacagatc caaaccatgg
    12361 cagccaaatt ggcctcgaca gggctaacac atgcccgtat ggatggacac accgataact
    12421 atggtgaaga cagttacaac gaaggcttat cattgaaacg ggcgaatgtc gtggccgatg
    12481 catgggctat gggtggacaa attccacgca gcaatctcac cacacagggt ttaggaaaaa
    12541 aatatcccat agccagtaac aagaccgccc agggccgcgc cgagaaccgc cgcgtcgcag
    12601 tggtgattac tcccccttaa aacacgagca aacagataaa aaaagggcca gccaattggc
    12661 cagcccttct taacaggatg tcgcttaagc gaaatcttag ttaagacgct ctttgatacg
    12721 agcagcctta ccagtacgct cacgcaggta gtacagttta gctttacgaa cagcaccacg
    12781 acgtttgaca gaaatgctgt caactaccgg agagtgagtc tggaagacac gctcaacgcc
    12841 ttcgccgttg gaaattttac gaacagtgaa tgcagagtgc agaccgcggt tacgaatagc
    12901 gataaccacg ccctcgaatg cctgcagacg ttttttggaa ccttcaacaa cccatacttt
    12961 cacttccacg gtatcacccg gacggaagga aggtacgtcc tgcttcatct gctcttgttc
    13021 aagttgctta ataatgttgc tcataattta atctcttatc ctgggtaaac tgatatctcg
    13081 ggggcttacg ccatcccatc atgtttatgt tgctgttgtg cgtgttccgt tttgaactcc
    13141 gccagcaacc ttgcttgctc ttcagtcaga gccaggtttt ccagaagttc aggtcttcta
    13201 agccaggtac ggcccagcga ctgtttcaaa cgccagcgac gtatctcggc atggttgccc
    13261 gacagtaaca ctggcggaac ttccatccct tctaacacct caggccgcgt atagtgcggg
    13321 caatccagca atccttcagc aaaggaatct tccgttgccg aggcttcatg tcccagtact
    13381 cccggaataa accgggaaac ggagtcaatc agcgtcattg ctggtaactc accaccactg
    13441 agaacgtaat cgccgattga ccattcttcg tcaatttcgg tttggatcac gcgctcatct
    13501 ataccttcgt agcgaccgca caccagaatc aatttttgat tcgttgccag ttcgctgacg
    13561 cccgcttgat caagcttgcg tccctgtggt gaaagataaa tcacctttgc gccttcaccc
    13621 gccgcggctt ttgctgcatg aatggcgtcc cgcaagggtt gcaccatcat taacatcccc
    13681 ggtccgccgc cgtaaggacg atcgtccacg gtacggtgcc ggtcatgcgt gaaatcgcga
    13741 ggactccagc tctggatgct cagcaggcca tttttaactg cccggccagt taccccgtaa
    13801 tcggtaattg cgcggaacat ttcaggaaac aggctaatta tgccaatcca catagcgccg
    13861 tcttttaccg tttatccggt ggtttaaaaa ccaggatccc aatctacttc gattgaacga
    13921 gtagtgagat cgactttctt gataacctgc ccatcgagga acggtacgag acgttccttg
    13981 ataccaaacg catctttcag gtttgcctta atgacgagaa cgtcattgga tccggtttcc
    14041 atcatatcga cgactttacc gagatcgtag ccttcagtgg ttactacctg gcagcccatc
    14101 aggtctttcc agtagtagtc accctcttca agctgaggca gctgcgatga atccacgaca
    14161 atttcacaat tcgtcagcag gttcgccgca tcacgatcgt caacgccttt cagcttgatg
    14221 atcatgtcct gattgtggtg cttccagctt tccagctgga cttgctgcca ctgacccgcc
    14281 ttctggataa accagggctg atagtcaaaa atgctttcgg cgtcttcggt ggaagaaaac
    14341 actctgagcc acccacgaat accgtaagac gaacccattt ttcccaaaac gatgggatca
    14401 acaggtgctt gcgcggtgag ttgtttgctc atcatgacca ccgtgacaga ttaagctgct
    14461 ttgtttactt ctttgatcag cgcagcaacg cgatcagaaa tagttgcgcc ctggccaacc
    14521 cagtgagcga tgcgatccag atccaggcga gtgccttctt ctttttcgct agcgattggg
    14581 ttgaagaaac caacgcgctc gatgaagcga ccgttgcgtg cattacggct gtcagcgaca
    14641 acaacctggt agaacggacg ctttttagcg ccgtgacgtg ctaaacgaat agttaccata
    14701 acatcctctt gtgtgaataa aacaaccgga ccccatcgag gaacggagtc cggtgtcata
    14761 ttaaaagccc gaaaatttta ctgatttctg gagaaaatgc aatcagcagt taataactct
    14821 gtcgcataag gtcatttagc gaccaggaaa tcccggcggc atcatcccct tcatgcttct
    14881 catcatcttc gccattccgc ccttcttcat tttcttcatc atgcgctgca tgtcgtcgaa
    14941 ctgtttcaga agacggttaa cgtcctgcac ctgcataccg caaccggcag caatacggcg
    15001 tttacgcgaa cctttgatga tttctggctt agcgcgctct ttcatcgtca tcgagttgat
    15061 gatggcttcc atacgcacca gcactttatc gtccatctgt gacttgacgt tatccgggat
    15121 ctgccccatg cccggcagtt tgcccatcag gctggccatg ccgcccatat ttttcatctg
    15181 gcgcagttgc tcaaggaagt cgttgagatc gaagccgtca ccttttttca gcttgctggc
    15241 taatttctct gcctgcgcgc ggtcaacttt gctttcgata tcttcgatca gcgacagtac
    15301 gtcgcccatg ccgagaatac gcgacgcgat gcggtccgga tggaacggct ccagcgcctc
    15361 agttttctcg ccaacaccga ggaacttgat cggtttgcca gtgatgtgac gaatagagag
    15421 cgccgcaccg ccgcgggcat cgccgtccac tttggtcaac actacgccgg taagcggtaa
    15481 cgcttcattg aatgcttttg ccgtattggc cgcatcctga ccggtcatgg cgtcaaccac
    15541 aaacagggtt tcaaccgggt taatcgacgc atggacttgt ttgatctcgt ccatcatcgc
    15601 ttcgtcaacg tgcagacgac cagcggtatc caccagcagc acgtcgtaga atttcagttt
    15661 ggcttctttc agcgccgcgt taacaatatc aaccggcttc tgaccaacat cagaagggaa
    15721 gaaatcaacg cccacctgct ctgccagcgt ctcaagctgt ttgattgccg ccgggcgata
    15781 gacgtcagca gaaaccacca gcactttctt cttgtgcttc tcgcgcagga acttaccgag
    15841 tttaccaacg ctggttgttt taccggcacc ttgcaggcct gccatcagca cgaccgcagg
    15901 cggttgcgca gccagattca gggtctggtt ctcttcgccc atcgccgcaa ccagttcgtt
    15961 acggacgatt ttgacgaact cctgccccgg cgtcaggctc ttattaactt catgaccaac
    16021 cgctttctct tttacgcgat tgataaactc acgcactacc ggcagagcga cgtccgcctc
    16081 cagcagcgcc atgcgcactt cgcgcagcgt atcttttacg ttgtcttcag tgaggcgtcc
    16141 acggccactg atattgcgca gcgtgcgcga caaacgatcg gttaaattat caaacattgt
    16201 ctctcgcctg gggtggaaac ggttggccgc aatcgcgaca catcatcagt attttgccgc
    16261 agtataacat gaaggcgtct ttgttgttat gcaacggttg gagcagcgtt cacctgacgc
    16321 tatactgctt ctctttctta ttgctcaaac tgtcgacatc actatgcccg tttttgctct
    16381 gctcgcgctt gtcgcctact ccgtcagtct tgcgctgatt gttcccggtc tgctgcaaaa
    16441 aaacggcggc tggcggcgca tggctattat ttctgcggtc attgcgctgg tctgccacgc
    16501 aatcgctctg gaagcccgca tcctgcccga cggtgatagc ggacaaaacc tcagcctgct
    16561 gaacgttggt tcattggtca gtttgatgat ctgtacggta atgaccattg tggcttctcg
    16621 caatcgtggc tggttgctgc tacccattgt ctatgccttt gcgcttatca acctggcgct
    16681 ggcaaccttc atgcccaatg aatacatcac ccatctggaa gctacgcctg ggatgctggt
    16741 gcacattggc ttatcgctct tttcctatgc cacgctaatt atcgccgccc tgtacgcgct
    16801 gcaactggcg tggattgatt accaactgaa gaacaagaag ctggcgttta accaggaaat
    16861 gccgccattg atgagtatcg agcgtaaaat gttccacatc acgcagattg gcgtggtgct
    16921 gctaacgctc acgctttgca ctggcctgtt ctacatgcac aacttattta gcatggaaaa
    16981 tatcgacaag gctgtgctct ctatcgtggc gtggtttgtc tatattgtgc tgctatgggg
    17041 acattatcat gaaggatggc gtggacgtcg cgtcgtctgg tttaacgttg cgggtgcagt
    17101 cattctgaca ctggcctact tcggcagccg aattgtccag cagttaatca gctaaaccca
    17161 gaaaaggagt ttccccctgg aacacatttc tactactacg ttgatcatta ttctgatcat
    17221 catggtggtc atttcagcct atttttccgg gtccgaaacc ggaatgatga ccctcaaccg
    17281 ctatcgtctg cgacatatgg cgaaacaggg taatcgctcg gccaaacgcg tcgaaaaatt
    17341 gctgcgtaag ccagaccgcc tgataagcct ggtgttaatc ggcaataacc tggtcaatat
    17401 tcttgcctcc gcgctcggca ctattgttgg gatgcgtttg tacggcgatg cgggcgtggc
    17461 aattgcgact ggtgtgctga cttttgtcgt actggttttt gctgaggtat tgccgaaaac
    17521 cattgccgcg ctgtacccgg aaaaagtcgc ttatccgagt agttttctgc tggctcccct
    17581 gcaaattttg atgatgccgc tggtctggtt gctgaatgct atcacccgta tgctgatgcg
    17641 catgatgggt atcaaaaccg atatcgtggt tagcggctct ttgagcaaag aagagttgcg
    17701 cactatcgtg cacgaatcgc gctcacaaat ttcccgtcgc aatcaggata tgctgctgtc
    17761 ggtgctcgat ctggaaaaaa tgaccgttga tgacatcatg gtgccgccag tgaaattatc
    17821 ggtattgata tcaacgatga ctggaaatct attctgcgcc aactctccca ctcacctcac
    17881 gggcgcatcg tgctctaccg tgattcgctg gacgacgcca tcagtatgct gcgagtacgt
    17941 gaagcttggc ggttgatgtc ggagaaaaaa gagttcacca aagaaaccat gctgcgcgcc
    18001 gcggacgaga tctattttgt gccggaaggt acgccgctca gcacgcagtt ggtgaagttt
    18061 cagcgcaaca aaaagaaagt cggcctggtc gtcaacgagt atggagacat tcaggggctg
    18121 gtgacggttg aagatattct ggaagagatt gtcggcgatt tcactacgtc gatgtcgcca
    18181 acacttgccg aagaagtcac gccgcaaaac gacggttcgg tgattattga tggcactgcc
    18241 aacgtgcggg aaatcaacaa agcctttaac tggcatctac cggaagatga tgcccgcacg
    18301 gttaatggcg tcattcttga ggcactggaa gagatccctg tcgtaggcac ccgcgtgcgt
    18361 attggcgagt acgatatcga tattctcgac gtacaggaca atatgattaa gcaggtaaaa
    18421 gtttttcctg tgaaaccgct gcgcgagagt gtggcggagt aacgaaaacg gcccggcatt
    18481 cgcatgcagg gccgtgaatt attacgaaaa cagaaattac gcttttactt tcgctacagt
    18541 taccatcgcc gcacgaatcg tacgaccatt cagcgtataa cccttctgca taatgcccag
    18601 tacgttacct ggcgcaacgt catcagattc caccattgcg atagcctgat gcacattcgg
    18661 gtccagcggc acgttagttt cggcgatcac ttcaacacca aacttacgca caacatccag
    18721 catcgacttc agcgtcagct caatgccttc aaccatcgca gacatatccg ggttagcttt
    18781 atcagccact tccagcgcac gatccaggct atcaatcacc ggcagcaatt cgttgatgaa
    18841 tttctccagc gcgaatttgt gggctttttc aatatccagt tcagtacgac gacgcaggtt
    18901 ttccatttcg gcttttacac gcaaaatgcc gtcacgttca cgggtctggg cttcagccag
    18961 ctgagcttcg agattcgcaa ttttttcatc gcgcggatcc acctgctcag cagaagcttc
    19021 tggctcaact gcctcaatct cttcgtgctg atccatgata atttcttccg gggcttgccc
    19081 ctcaggcgtt ttctgttctt tactactcat gaatttctcc gcgttttttt cgcattcatc
    19141 tcgctaactt cgcttattat ggggatcagt ttcagggttt caagggaagc actcacattg
    19201 tcatcaatct tcgcaacaag gacctcggaa aaatgaataa tcatttcaag tgtattggca
    19261 ttgtgggaca cccacggcac cctactgcac tgacaacaca tgaaatgctc taccgctggc
    19321 tgtgcacaaa aggttacgag gtcatcgttg agcaacaaat cgctcacgaa ctgcaactga
    19381 agaatgtgaa aactggcacg ctcgcggaga ttgggcaact ggctgatctc gcggtagtcg
    19441 ttggtggcga cggtaatatg ctgggcgcga cgcgcacgct cgcccgttac gatattaaag
    19501 ttattggaat caaccgtggc aacctgggtt tcctgactga ccttgacccc gataacgccc
    19561 agcaacaatt agccgatgtg ctggaaggtc actacatcag cgagaaacgt tttttgctgg
    19621 aagcgcaagt ctgtcagcaa gattgccaaa agcgcatcag caccgcgata aatgaagtgg
    19681 tacttcatcc aggcaaagtg gcgcatatga tagagttcga agtgtatatc gacgagatct
    19741 ttgcgttttc tcagcgatct gatgggctaa ttatttcgac gccaacaggc tccaccgcct
    19801 attccctctc tgcgggcggt cctattctga ccccctctct ggatgcgatt accctggtgc
    19861 ccatgttccc gcatacgttg tcagcacgac cactggtcat aaacagcagc agcacgatcc
    19921 gtctgcgttt ttcgcatcgc cgtaacgacc tggaaatcag ttgcgacagc cagatagcac
    19981 taccgattca ggaaggtgaa gatgtcctga ttcgtcgctg tgattaccat ctgaatctga
    20041 ttcatccgaa agattacagt tatttcaaca cattaagcac caagctcggc tggtcaaaaa
    20101 aattattcta attttacgcc agcctcttta ctgtatataa aaccagttta tactgtacac
    20161 aataacagta atggtttttc atacaggaaa acgactatgt tggcacaact gaccatcagc
    20221 aactttgcta tcgttcgtga gcttgagatt gattttcata gcggcatgac cgtaataact
    20281 ggcgaaaccg gtgcgggtaa atctattgca atagatgccc tcggtctttg tctcggtggt
    20341 cgcgctgaag ccgacatggt gcgtaccggc gctgctcgcg ctgacctgtg cgcccgtttt
    20401 tctctgaaag atacgccagc ggccctgcgc tggctggaag aaaaccagct tgaagacggg
    20461 catgaatgtt tgcttcgtcg cgtgatcagc agcgatggtc gctcccgtgg tttcatcaac
    20521 ggtacagctg ttcctctgtc acaactgcgc gaactgggtc agttgctgat tcagatccat
    20581 ggtcagcacg ctcatcagtt actcaccaaa cccgagcacc aaaaattcct gcttgatggc
    20641 tatgccaatg aaacctctct actccaggaa atgaccgcac gttatcagtt gtggcatcaa
    20701 agctgccgtg acctcgcgca tcatcaacag ttaagtcagg aacgcgctgc ccgtgcggaa
    20761 ctgctgcaat accaattaaa agaacttaac gaatttaatc cgcagcccgg agagtttgag
    20821 caaatcgacg aagagtacaa acgtctggcg aacagcggtc aattgctgac caccagccag
    20881 aatgcattgg cattaatggc cgacggtgaa gacgcaaacc tgcaaagtca gctttacacg
    20941 gctaaacaac tggtgagcga attgattggc atggacagca aactgtccgg cgtacttgat
    21001 atgctggaag aagctaccat ccagattgtt gaagccagcg acgaactgcg ccactactgc
    21061 gatcgtctgg atctcgatcc caaccgactg tttgaacttg aacagcgcat ctcaaaacag
    21121 atttcgctgg cacgtaaaca tcacgtcagc cctgagacat tgccacagta ttaccagtcg
    21181 ctactggaag aacagcagca actggacgat caggccgact cacaagaaac gctcgcactg
    21241 gcggtaacga aacatcatca gcaggcactg gaaacggcgc gcgcattaca ccagcaacgc
    21301 cagcattatg cagaagaact tgcacagctg atcaccgaca gtatgcatgc gctctcaatg
    21361 ccgcatgggc agtttacgat tgatgtcaaa tttgacgagc atcacctggg cgctgacggc
    21421 gccgatcgta ttgagtttcg ggtaaccacc aacccaggtc agccaatgca gcctattgcc
    21481 aaagtcgcat ccggtggtga attgtcccgc atcgcattgg caattcaggt catcacggcg
    21541 cgtaaaatgg aaaccccggc actgattttt gatgaagtgg atgtagggat tagcggtcca
    21601 acagcggcag ttgtcggcaa actgctgcgt caactcggcg aatcaactca ggtgatgtgt
    21661 gttacccacc tgccacaagt cgcgggatgt ggtcatcaac actattttgt cagcaaagaa
    21721 accgatggtg cgatgacaga aacgcatatg caatccctga ataaaaaaac gcggttacaa
    21781 gagctggcgc gcctgcttgg tggcagtgaa gtcacacgta acacactggc gaatgcgaaa
    21841 gaactgcttg cggcgtaaac ttttttcctg cttcacggtc agagtaaaca gcaaaacgcc
    21901 gtaagaccgg aaagcaaaag gttttaaagt gatgaaaggt ctattatcat cggcatatta
    21961 cagatgagcc acgtactgct cgggcccgaa aaggaatcaa atcactatgc gctgtaaaac
    22021 gctgactgct gcagcagcag tactattgat gttgaccgca ggctgttcca ctctggagcg
    22081 agtggtttac cgtcctgaca tcaaccaggg gaactatctg accgctaacg acgtatccaa
    22141 aatacgtgtt ggcatgacgc aacaacaagt tgcgtacgca ttgggtacac cgctgatgtc
    22201 cgatccattt ggtacgaata cctggttcta tgtcttccgc cagcaaccag gtcatgaagg
    22261 tgtaacgcag caaacgctga cgctaacctt taacagtagc ggtgtgttga ccaatattga
    22321 taacaaacct gcgctgagtg gtaactaata ataaagttgc tctcaaagac gttaaaaaag
    22381 gtgctcaatg agcacctttt ttctgtccgt tatttattcg ctgatttttc tgctcgttgc
    22441 ctgcgaagct ctttcggatc ggcaatgaga gggcgataaa tctccacccg atcgccatca
    22501 tgcacactat cgcttaattt tgccggacgg ctgtaaatgc cgactttgtt ttcagttaaa
    22561 tcgatatcgg tacgcaattc cagcaagcca ctggcgcgaa tagcttcttc aaccgtcgcg
    22621 ccctcctgca gcgtcacgcg ctgcaggtac tgcttcttag gtagcgcata agccacctca
    22681 acggcaattt tacctggcac tgtaaacctc tttcgcacga accgtaaaag cctggaccat
    22741 attagacgcc agctctttaa acacgcgacc aaaagcgagt tcaatcaact tattggtaaa
    22801 ctcaaagtcg agatgaaact cgatacgaca cgcctcctgg ctcagcggcg taaacttcca
    22861 tccaccaatc aatttcttga acggcccatc caccagattc ataagaatac tttggttact
    22921 ggtcaactgg ttgcgggtag taaacgtttt gctgatccca gccttagaga catctaccgc
    22981 cgcagtcatc tgcccaggag tggactccag aatccgactt ccggtacaac ccggcaaaaa
    23041 ctgaggataa gactgaacgt cattcactaa ctgatacatt tgctccgcgc tgtagggtac
    23101 cagtgcggtc cggctaatct gaggcataac aatttccatc aacaaaaatc caacaaataa
    23161 tatcatttat cctgcagtaa aaaaaacgct atcccggcgc tgggtaacat ccggttcatg
    23221 ctaagataga gccttgtccc ccgcaggatt gatatggggt attttcgatt tcagattacc
    23281 gatgattcac gacgcttatg acgaagaaaa aagcacataa acctggttca gcgaccatcg
    23341 cgcttaacaa gcgcgcccgt cacgaatact ttattgaaga agagttcgaa gcgggacttg
    23401 ccctgcaagg ctgggaagtt aaatccctgc gcgcaggaaa agccaatatt agcgacagct
    23461 atgtccttct gcgcgacgga gaagcgttcc tgtttggcgc aaacatcacg ccaatggctg
    23521 tggcttctac gcatgtggtg tgcgatccta cccgtacccg caagttactt ctcaaccagc
    23581 gcgaacttga ttcgttgtac ggtcgcgtca atcgagaagg ctataccgta gtggcgctct
    23641 ccctgtactg gaaaaatgcc tggtgcaaag tgaaaatcgg cgtcgccaaa ggtaagaaac
    23701 agcacgataa acgttcagat atcaaagaac gcgaatggca ggtggataaa gcgcgtatca
    23761 tgaaaaacgc ccaccgttaa acctgcactc caattcttga cctgttcctc accgcgcctc
    23821 cctctccggc ggcgcgaatg aacatcttat tggctatcac atccgacaaa aatgttgcca
    23881 tcccattgct taatcgaata aaaatcaggc tacatgggtg ctaaatcttt aacgataacg
    23941 ccattgaggc tggtcatagc gctcataaat ctggtatact tgccgttaca cattggggct
    24001 gattctggat tcgacgggat ttgcgaaacc caaggtgcat gccgaggggc ggttggcctc
    24061 gtaaaaagcc gcaaaaaata gtcgcaaacg acgaaaacta cgctttagca gcttaataac
    24121 ctgcttagag ccctctctcc ctagcctccg ctcttaggac ggggatcaag agaggtcaaa
    24181 cccaaaagag atcgcgtgga agccctgcct ggggttgaag cgttaaaact taatcaggct
    24241 agtttgttag tggcgtgtcc gtccgcagct ggcaagcgaa tgtaaagact gactaagcat
    24301 gtagtaccga ggacgtagga atttcggacg cgggttcaac tcccgccagc tccaccactt
    24361 taagaaggac tacaaccgga cagtagcaat aaatacagcc acttacggac acaagccaga
    24421 cagtagtcgg actgaaaaaa gacaaaaata tacacgtgaa atgcacgtgc acttctaaaa
    24481 aaccccagtt atcaccgact ggggtttttc aatttgtaac taagggtaac aacccccccg
    24541 tttttttcat ctcactcacc ttgatactgt ttatttgtac agttaaaaat aatactgtat
    24601 acaaacacag tatagaggga cttttatgcg tattgagatc tgcatagcca aagaaaaaat
//
LOCUS       DBFIFM010000054        24148 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.054, whole genome shotgun sequence.
ACCESSION   DBFIFM010000054 DBFIFM010000000
VERSION     DBFIFM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.054"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(12..893)
                     /gene="prmA"
                     /locus_tag="N7C74_001894"
     CDS             complement(12..893)
                     /gene="prmA"
                     /locus_tag="N7C74_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HGU2431771.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(905..2356)
                     /gene="panF"
                     /locus_tag="N7C74_001895"
     CDS             complement(905..2356)
                     /gene="panF"
                     /locus_tag="N7C74_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HGU2431772.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(2346..2588)
                     /locus_tag="N7C74_001896"
     CDS             complement(2346..2588)
                     /locus_tag="N7C74_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HGU2431773.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(2697..4046)
                     /gene="accC"
                     /locus_tag="N7C74_001897"
     CDS             complement(2697..4046)
                     /gene="accC"
                     /locus_tag="N7C74_001897"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HGU2431774.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNSIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(4057..4527)
                     /gene="accB"
                     /locus_tag="N7C74_001898"
     CDS             complement(4057..4527)
                     /gene="accB"
                     /locus_tag="N7C74_001898"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HGU2431775.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5505..6479)
                     /locus_tag="N7C74_001899"
     CDS             complement(5505..6479)
                     /locus_tag="N7C74_001899"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HGU2431776.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            6631..8571
                     /gene="csrD"
                     /locus_tag="N7C74_001900"
     CDS             6631..8571
                     /gene="csrD"
                     /locus_tag="N7C74_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HGU2431777.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            8876..9919
                     /gene="mreB"
                     /locus_tag="N7C74_001901"
     CDS             8876..9919
                     /gene="mreB"
                     /locus_tag="N7C74_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HGU2431778.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            9985..11088
                     /gene="mreC"
                     /locus_tag="N7C74_001902"
     CDS             9985..11088
                     /gene="mreC"
                     /locus_tag="N7C74_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HGU2431779.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSLQRATPPQSGAQPPARAPGGQ"
     gene            11088..11576
                     /gene="mreD"
                     /locus_tag="N7C74_001903"
     CDS             11088..11576
                     /gene="mreD"
                     /locus_tag="N7C74_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HGU2431780.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            11585..12178
                     /gene="yhdE"
                     /locus_tag="N7C74_001904"
     CDS             11585..12178
                     /gene="yhdE"
                     /locus_tag="N7C74_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HGU2431781.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKARAGVVQTAKDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSRHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYQAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            12168..13637
                     /gene="rng"
                     /locus_tag="N7C74_001905"
     CDS             12168..13637
                     /gene="rng"
                     /locus_tag="N7C74_001905"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HGU2431782.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            13705..17505
                     /gene="yhdP"
                     /locus_tag="N7C74_001906"
     CDS             13705..17505
                     /gene="yhdP"
                     /locus_tag="N7C74_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HGU2431783.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLGIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIVLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWSDAQIRCAECWFWGQKFGRIDSDITISGNTLTLTNGLI
                     DTGFSRLTADGEWINNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            17661..19106
                     /gene="tldD"
                     /locus_tag="N7C74_001907"
     CDS             17661..19106
                     /gene="tldD"
                     /locus_tag="N7C74_001907"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HGU2431784.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFVILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIARD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLIEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(19240..20169)
                     /gene="aaeR"
                     /locus_tag="N7C74_001908"
     CDS             complement(19240..20169)
                     /gene="aaeR"
                     /locus_tag="N7C74_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HGU2431785.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            20352..20555
                     /gene="aaeX"
                     /locus_tag="N7C74_001909"
     CDS             20352..20555
                     /gene="aaeX"
                     /locus_tag="N7C74_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HGU2431786.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVCRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            20563..21492
                     /gene="aaeA"
                     /locus_tag="N7C74_001910"
     CDS             20563..21492
                     /gene="aaeA"
                     /locus_tag="N7C74_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HGU2431787.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQE
                     KRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAPA
                     DGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGSN
                     KVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWPA
                     GTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            21498..23465
                     /gene="aaeB"
                     /locus_tag="N7C74_001911"
     CDS             21498..23465
                     /gene="aaeB"
                     /locus_tag="N7C74_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HGU2431788.1"
                     /translation="MDIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LCIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            23557..23829
                     /locus_tag="N7C74_001912"
     CDS             23557..23829
                     /locus_tag="N7C74_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HGU2431789.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(23885..24148)
                     /gene="yhcN"
                     /locus_tag="N7C74_001913"
     CDS             complement(23885..24148)
                     /gene="yhcN"
                     /locus_tag="N7C74_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HGU2431790.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         5712 a         6097 c         6583 g         5756 t
ORIGIN      
        1 cgatgcgaag gttaattctt acgaccggta atacggcacc actcttcttt ttccacgacc
       61 gggtccagag cgaagctgtc ggcataggct tcgcaaacgc tctccgcctg gcttgccaga
      121 ataccagaaa ggcccagtaa gccgcctgaa actggcagga cgctaattaa cggtgccagt
      181 tcacgtaatg ggcctgcaag gatgttagcg accaccacgt cggctttcat ttcttctggc
      241 tgatctttcg gtaagtagag ttccagacgg tcagaaacgc cattacgttc ggcgttatcg
      301 cggctggcct gaatcgcctg cggatcgata tcaataccaa tggcttttgc tgcacccagt
      361 ttcagcgccg cgatcgccag aatgccggaa ccacagccaa agtcgatgac tgttttaccg
      421 gttaaatcga ggctgtcgag ccattgcagg cacagagagg tggttggatg ggtacccgta
      481 ccaaacgcca gccctggatc taacatcacg ttgacggcgt tttcgtccgg cacatcacgc
      541 cagctagggc agatccacag tcgttcgcca aagcgcatcg ggtggaaatt atccatccat
      601 tcgcgctccc agtctttatc ttctagttgt tcgattttat gcgcgaagcc tgcgccgagc
      661 agcggatggt tttccagaat cgccaccacg tcgttcatat cggtttcagc gtcgaacaga
      721 ccaatcacat cggtgtcgcc ccacaggcgc gtttcgcccg gcagcggctc aaatactggc
      781 gtatcgtggg tatcctgaaa agtgatagaa acggcacccg cttccatcag cgcatcgcta
      841 agatcttccg cgttcgcgcc ggtggtgttc agtttcagtt ggatccaagg catggcaaaa
      901 ctctttattt atcagtagtc aaaacggtag cttgcgggac ggatgtaccg aaacggtttc
      961 cgaccaggaa agccagcaaa cttagtagta acgagggcac gatagggtgg aagcccaggt
     1021 actgaatatt cagcgtcgcg agtacggcat acagcacgcc gccaacgatc atcgcactta
     1081 gcgcgccttt ggcgttggcg cgttcccagt aaagacccag caccagcggc cacaggaaaa
     1141 cggcttccag cccaccgaag gccagcaaat tcagccagat gatcatttct ggcggcttcc
     1201 aggcggcaag cagcagcaac gcgccgagaa ctaacgtaat taccgccgac atccgcttca
     1261 gacgcgtctc gttttgcatt tgatccggac ggatattcag atagagatct ttaatgatcg
     1321 tagcggaact ttgtagcaat tgggcgttaa ttgtcgacat gatcgcagcc ataggtgcag
     1381 ccagaaagat cccggcagca aacggtggca gcacttttac cattaacgtc gggatcacca
     1441 gatccggtac ggtgagatcg gggatcactg cacgacctaa cgctccggct aggtgcatac
     1501 cgaacatcag gattgcgact acaatcgtac cgatgatgat cccccggtgt actgctttgc
     1561 tgtctttata agagatacag cgcaccgcag tatgcggcag gcctataacg ccaaagcaca
     1621 ccagtaccca gaacgacgtc ataaaggcag gcgacagaat atcgtcagcg ccttgtggcg
     1681 taaccagttg cggatcgatg gtttgcaagg tctgtactgc gttgcttaag ccgccagcgg
     1741 catgtaccac gccaataagc agcacaacgg tgccaatcag catcacaagc ccttgcatgg
     1801 tgtcgttcag cacgctggcg cgaaagccac caaaggcggt atataacgca atgctgatac
     1861 caaaaatcag cagcccggtt tcataaggaa tacccgccgc ggtttccagc aggcgcgcac
     1921 cgccgataaa ctgcacggtc atcgcaccaa cgaacgcaac cagcaaactc aaactcgcca
     1981 gccacaccag aagacgactc tggtagcggg caaacagcat atcgttcagc gtcactgcat
     2041 tgtagcggcg cgcaagaatc gcaaacttct tgccgagaat accgagtgaa agccagactg
     2101 ccggaagctg aatcatcgcc agcaataccc agccaaggcc gtatttgtaa gcagctccag
     2161 gcccgccgat aaacgaactg gcactgatat aagtcgcggt gagcgtcatc gccagcacaa
     2221 taccgcccat agagcggctg ccgaggaaat actcattaag gaaggtgccg gtgctccgtt
     2281 tacgcatcgc ataaaccgag ataccgaaca ccaccaccag ataggcgacc agcggtagaa
     2341 ttacttcaag ctgcatcgtc atcctccagc gggatatcgc gatagataaa tttcaccatc
     2401 gcccagcaca gtccaataaa cagcagcggc gtcaggatgc aggccatctc aaaccagcgc
     2461 ggaaagccgg taaaaccggg ggcaacgcca ggtaagtaag cggctactaa ccaaactgcc
     2521 agatacaaaa gggtcagccc cagcgcccag cgcgcctctt tatgggcctg aacaaaacga
     2581 gtgtccattt tttgtccctg atgggtgaag aaagcgagga ttgtacctta tgggtgttgt
     2641 cgatccccag taataaaaaa ggccggaaaa tccggccttt tgacgctttt gcagtcttat
     2701 ttttcctgaa gaccgagttt tttctccaga tagtggatgt tagtgccacc atgctggaag
     2761 ttctcgtcat tcatgatgcg gatctgcaga tcaacgttgg ttttgatacc gtcgatgatc
     2821 agctcctgca gcgcattctt catgcgggca atcgccacgt cacggttttc accgtagcaa
     2881 atcagcttac cgatcattga gtcatagtac ggcggtacgg tgtagcccgc gtagatatga
     2941 gactcccaac gtacgccaaa accgccaggt gcgtggaaac gggtgatttt gcccggactt
     3001 ggcaggaagg tgttcggatc ttcggcgttg atacgacatt ccaccgcatg gccgcgaacg
     3061 tgaacttctt cttgcttgat cgacagcggt tgaccggcag cgatacgcag ctgttctttg
     3121 atcaggtcaa cgccggtgat catttctgta accgggtgtt ctacctgaat acgggtgttc
     3181 atttcgatga aatagaactc gccgttttcg aacaggaact cgaaagtacc tgcaccgcga
     3241 tagccgatat caacacacgc tttagcgcaa cgttcgccga tgtagcgacg cagttccggg
     3301 gtaatgcccg gtgctggtgc ttcttcgacc actttctggt ggcggcgctg catggagcag
     3361 tcacgttccg ccagatagat agagttgccc tgaccgtcag ccagtacctg aatctcgacg
     3421 tggcgaggat tttccaggta tttctccatg taaaccatat cgttgctgaa agcagctttc
     3481 gcttccgcac gggtcatgga gatggattgt gccagttcag cgtcgccgcg cactacgcgc
     3541 ataccgcgac caccgccgcc gccggaggct ttgataatca ccggataacc aatgcgttta
     3601 gcaatggcac ggtttttatc catatcgtcg cccagcgggc cgtcagaacc cggtacgcaa
     3661 gggacgcccg cttttttcat cgccgcgatt gcggatactt tgtcgcccat caggcgaatg
     3721 gtttctgctt tcgggccaat gaagataaag ccggagcgtt caacctgctc ggcaaagttg
     3781 gcgttctcgg agaggaagcc gtaacccgga tggattgcta ctgcgccggt gatttcagcg
     3841 gcgctgatga ttgccgggat gttcagataa ctttttactg acggagcagg gccaatacag
     3901 accgtttcat ctgccagtaa tacgtgtttt agatcgcgat ccgcgctgga gtgcacagcg
     3961 acagtcttga tgcccagttc tttacaggca cgaagaatac gcaatgcaat ctcgccgcgg
     4021 ttggcaataa caattttatc cagcatgttc gcctcgttac tcgatgacga ccagcggctc
     4081 gtcaaattct accggttgtc cactttcgac cagaattgct ttcacggtac cggatttgtc
     4141 cgcttcgatc tggttcatca ttttcatggc ttcaacgatg cacagggtat cgcccacgtt
     4201 gactttctga cccacttcga tgaacgcttt tgcatccggg cttggggtgc ggtagaaagt
     4261 accaaccatc ggggaacgta cgatgtgacc actgatttcc gctgctgctg gcgcttccat
     4321 ggaaggaacg gtcgccggag cggctgcgtt agattgagct ggctgctgca tcattggtgc
     4381 agcgtaagct tgttgcatca cagggaaact tgcggcagga gctgcacggc taatgcgtac
     4441 tgactcttcg ccttcagaaa tttccagttc ggagatgcct gattcttcaa ccagctcgat
     4501 cagtttttta atcttacgaa tatccatgag tgggttccgt actctttgtt tagtgtgatt
     4561 gtgacaggcg tttcaccgcc gtctgtaaag cgtatgcccc gacagctgta tgcatagcga
     4621 taaattccag caggtcggga cgccggtcta ttttgcccca gagtgtcaat gttagacttg
     4681 acggacattg tgcagcgatc tgctgctatc ctccggcaaa aaacaaaata taccttcgtt
     4741 gcgccaatgt caccttttcc gcagcgcaaa aactgcgtca gggaggacga ggtcgcacat
     4801 tataacgatt tcgtagcaat tggcagctaa atactggtct tatcagggaa gataatcaac
     4861 aactaacatg taaataacct tcaacaccgt gtaatttgca acaagccgca caattcacga
     4921 aattagcgcc atcatcgacg gaacttctta taacgtaagg tgaaaagctg taaagccagc
     4981 cctgcgaaga tgaggggctg caatgagata atcgtcacag accacaaata atgtagggat
     5041 gccaggatcg cgacaagata gacgacatta cgcagcggtt gctggggatt acccaatttt
     5101 cactgcatcg cctggataga tgtgaatgct aaagcacgca aaattaaccg attgacaatg
     5161 ctcagcatta aataggttcg agtgattaat ttgtagtcaa acaaaatcag attattcacc
     5221 tcatggtacc agaaataagt aaggggttaa gcgtagcgtc gcccaggcaa agcaccataa
     5281 acctaacagg cgacggaggc gtatcaataa tagctgttta gtggagtatc aatgcgcatc
     5341 aatttatggt cagtcgaaat catgactttc cagtttggat gatgttgtac aacttcatga
     5401 ccaaggtgtt cgacgaaagc tgtccggcta accgcttttc ccgttaaccg attgaataat
     5461 gatttcaaaa tgagttccct ctcttattat tccttgctaa atggttagtt aaccttcacc
     5521 agcgtgcgac cctggatctg gttattaatg atggcctcgg caaagttcgg tgcctctgac
     5581 agagatatct cttttgccgc ctgggtatag aatgattccg gtaaatcggc gaccagtcgc
     5641 tgccaggctt gtgcgcggcg ttctggtggc gtcattactg aatccacccc ttgcaaacgg
     5701 acattacgca gaataaatgg catgaccgtg gttggcagag taaaaccacc cgccagacca
     5761 caggccgcca cgcagccgcc gtaattcatt tgcgccagca cttttgccag cactttgtcg
     5821 ccaacagtgt caattgcccc agcccagact tgtttttcca gaggacggga ttcggcaaac
     5881 tcatcacgag ggagaatacg gctggcacct aaacttttca gatattcatg ggtactttcg
     5941 cgaccggaaa cggcaacgac ctgataaccc aacttatgca gcagcgccac ggcggtactg
     6001 ccgacgccac cactggcacc cgtcacgaca atctccccgt cctgcgggcg aacaccggca
     6061 tcttccagcg ccatcacaca cagcatggcg gtaaaaccgg cagtaccgat aatcattgct
     6121 ttacgcgcgt ccagcccttg cggcatggca accagccagt cacctttcac tcgcgcctgc
     6181 tccgccagcc caccccagtg gttttcacca acgccccagc cagtgagtaa cacctcctga
     6241 ccggcatgaa aacgcggatc ttcgctggtg cgtacagttc cggcaaaatc gatcccagga
     6301 atcatcggaa aattacggat gatttttccc ttaccggtaa tcgccagcgc atctttatag
     6361 ttcaggctcg accagtgaac atcgaccgtg acatcgccct ccggcaggcg actttcgtcc
     6421 agagtctgta ctgatgcgag agttttgccg tcctgctgtt ctaaaagtaa cgcctgcata
     6481 agtggtcctc atgtgcatga tggattggaa aataatttct gaagactata ctcgctaatg
     6541 gaaaagcaaa gccgatgaag cgcaataaat tgcgagataa atctgatttg ctagtatgcc
     6601 cgcttcctca ctatcggagt taacacaagg atgagattaa cgacgaaatt ttcggccttt
     6661 gttacgctgc tcaccgggtt aacaattttt gtgactttgc tgggctgttc gctaagtttc
     6721 tacaacgcca ttcagtataa gtttagtcat cgcgttcagg cggtggcgac ggcgattgat
     6781 acccaccttg tgtcgaatga cttcagcgca ttaaggccac aaattaccga attaatgatg
     6841 tcggcagata tcgttcgtgt agacctgctc catggtgata agcaggttta taccctggcc
     6901 agaaatggta gttatcgtcc ggttggcacc agcgatctgt ttcgtgaact gagcgttccg
     6961 ttgataaagc atccggggat gtcgttgcgt ctggtttatc aggatccgat gggcaactat
     7021 ttccattcgt tgatgaccac cgcgccgctc acaggggcga ttggctttat cattcttatg
     7081 ctctttctgg cggtacgctg gttacaacgg caacttgccg ggcaagaatt gctggaaacc
     7141 cgggctactc gtatcttaaa cggtgagcgt ggctctaatg tgttgggaac catctatgaa
     7201 tggccgccca gaaccagcag tgcgctggat acgctgcttc gtgaaattca gaacgcacgc
     7261 gaacaacaca gccgtcttga tacgctgatc cgctcttatg ccgcacagga cgtgaaaacc
     7321 ggcctcaata accgactctt tttcgataat cagttagcaa cgttactgga agatcaggag
     7381 aaagtaggta cccacgggat cgtgatgatg attcgtctgc cggatttcaa tatgttgagc
     7441 gatacctggg ggcacagcca ggttgaagaa cagttcttca ctctgacgaa tctgctgtcg
     7501 acatttatga tgcgctaccc tggcgcactg ctggcgcgtt accaccgcag tgattttgct
     7561 gcgctgttac cgcaccggac gttaaaagag gcagagagca tcgccggtca gttaatcaaa
     7621 gccgtcgata ccttgccgaa caataaaatg ctcgatcgcg acgatatgat ccacattggt
     7681 atctgcgcct ggcgtagtgg tcaggatacc gagcaggtaa tggaacatgc agagtctgcc
     7741 acgcgtaatg cgggattgca gggcggcaat agctgggcta tttacgatga ctcgttgcct
     7801 gaaaaaggac gcggtaatgt tcgctggcgt acgcttatcg agcaaatgct cagtcgcggc
     7861 ggcccgcgcc tttatcaaaa accggcggtt actcgcgaag gtcaggttca tcatcgcgaa
     7921 ctcatgtgcc gcatctttga tggtaatgaa gaggttagct cggcggagta tatgccgatg
     7981 gtcttgcagt ttggcttatc ggaagagtat gaccgtctgc aaatcagccg tcttattcca
     8041 ctattgcgtt actggccaga ggaaaatctg gcgattcagg ttaccgttga gtcgctgatt
     8101 cgcccgcgtt ttcagcgttg gctgcgcgat acgttaatgc aatgtgaaaa atcacaacga
     8161 aaacgcataa ttattgaact tgcagaggcc gatgtaggtc aacatatcag tcgtttacaa
     8221 cctgttattc gtttagtgaa tgctttaggg gtacgggtag ccgtcaacca ggctggtttg
     8281 acgctggtaa gcaccagttg gatcaaagaa cttaatgttg agttactcaa gctccatccg
     8341 gggctggtca gaaacattga gaagcgaacg gagaaccagc tgctggttca aagcctggtg
     8401 gaagcctgct cagggaccag cacccaggtt tacgccaccg gtgtgcgttc gcgaagcgag
     8461 tggcagaccc tgattcagcg cggtgttaca ggcgggcaag gggatttttt cgcgtcctca
     8521 cagccacttg atactaacgt gaaaaaatat tcacaaagat actcggttta acctgccgtt
     8581 taatccgttt tcacgtagaa taacgcgcgc tgcgtctcat gggagtgtgc ttgtctgctc
     8641 gccagattgt tgcagcacat atgcagatga atgaccttac gcggttgcaa acaggcgagg
     8701 aatgctgctg atgcattaag cctttctgga ctcaggcaga gatttgtaac aaaggaaacg
     8761 aactgcacta attttcaccg tagcagatga tttttgcgcc ttgtcgctgc tgcgtgtggt
     8821 tggtaaagta agcggatttt cttttccgcc ccagctttca ggattatccc ttagtatgtt
     8881 gaaaaaattt cgtggcatgt tttccaatga cttgtccatt gacctgggta ctgcgaatac
     8941 cctcatttat gtaaaaggac aaggcatcgt attgaatgag ccttccgtgg tggccattcg
     9001 tcaggatcgt gccggttcac cgaaaagcgt agctgcagta ggtcatgacg cgaagcagat
     9061 gctgggccgt acgccgggca atattgctgc cattcgccca atgaaagacg gcgttatcgc
     9121 cgacttcttc gtgactgaaa aaatgctcca gcacttcatc aaacaagtgc acagcaacag
     9181 ctttatgcgt ccaagcccgc gcgttctggt ttgtgtaccg gttggcgcga cccaggttga
     9241 acgccgcgca attcgtgaat ccgcgcaggg cgctggtgcc cgtgaagtct tcctgattga
     9301 agaaccgatg gctgccgcaa ttggtgctgg cctgccggtt tctgaagcga ccggttctat
     9361 ggtggttgat atcggtggtg gtaccactga agttgctgtt atctccttga acggcgtggt
     9421 ttactcctct tccgtacgca ttggtggtga ccgtttcgac gaagctatca tcaactatgt
     9481 gcgtcgtaat tacggttctc tgatcggtga agccaccgca gaacgtatca agcacgaaat
     9541 cggttcggct tatccgggcg atgaagtccg tgaaatcgaa gttcgtggcc gtaacctggc
     9601 agaaggtgtt ccacgcggtt ttaccctgaa ctccaacgaa atcctcgaag cactgcagga
     9661 accgctgacc ggtattgtga gcgcggtaat ggttgcactg gaacagtgcc cgccggaact
     9721 ggcttccgac atctccgagc gcggcatggt actcaccggt ggtggcgcac tgctgcgtaa
     9781 ccttgaccgt ttgttaatgg aagaaaccgg cattccagtc gttgttgctg aagacccgct
     9841 gacctgtgtg gcgcgcggtg gcggcaaagc gctggaaatg atcgacatgc acggcggcga
     9901 cctgttcagc gaagagtaat cggatgcagg caggggaagc gtctgtttac cctgcctggt
     9961 ctgatacgag aatacgcata acttatgaag ccaattttta gccgtggccc gtcgctacag
    10021 attcgcctta ttctggcggt gctggtggcg ctcggcatta ttattgccga cagccgcctg
    10081 gggacgttca gtcaaatccg tacttatatg gataccgccg tcagtccttt ctactttgtt
    10141 tccaatgctc ctcgtgaatt gctggatggc gtatcgcaga cgctggcctc gcgtgaccaa
    10201 ctagaacttg aaaatcgggc gttacgtcag gaactgttgc tgaaaaacag tgaactgctg
    10261 atgcttggac aatacaaaca ggagaacgcg cgtctgcgcg agctgctggg ttccccgctg
    10321 cgtcaggatg agcagaaaat ggtgactcag gttatctcca cggttaacga tccttatagc
    10381 gatcaagttg ttatcgataa aggtagcgtt aatggcgttt atgaaggcca gccggtcatc
    10441 agcgacaaag gtgttgttgg tcaggtggtg gccgtcgcta aactgaccag tcgcgtgctg
    10501 ctgatttgtg atgcgaccca cgcgctgcca atccaggtgc tgcgcaacga tatccgcgta
    10561 attgcagccg gtaacggttg tacggatgat ttgcagcttg agcatctgcc agcgaatacg
    10621 gatattcgtg ttggtgatgt gctggtgacc tccggtctgg gcggtcgttt cccggaaggc
    10681 tatccggtcg cggttgtctc ttccgtaaaa ctcgataccc agcgcgctta tactgtgatt
    10741 caggcgcgtc cgactgcagg gctgcaacgt ttgcgttatc tgctgctgct gtggggggca
    10801 gatcgtaacg gcgctaaccc gatgacgccg gaagaggtgc atcgtgttgc taatgaacgt
    10861 ctgatgcaga tgatgccgca ggtattgcct tcgccagacg cgatggggcc aaagttacct
    10921 gaaccggcaa cggggatcgc tcagccgact ccgcagcaac ccacgacagg aaatgcagct
    10981 actgcgcctg ctgcgcccac acagcccgct gctaatcgct ctctacaaag ggctacgccg
    11041 ccgcaaagtg gtgctcaacc gcctgcgcgt gcgccgggag ggcaatagtg gcgagctatc
    11101 gtagccaggg acgctgggta atctggctct ctttcctcat tgcgcttttg ctgcaaatca
    11161 tgccctggcc ggataacctg attgttttcc ggccaaactg ggtgttactc atcttgttgt
    11221 attggatcct ggccttgccc catcgcgtaa atgtgggcac aggttttgtg atgggtgcca
    11281 tactggatct gatcagcggc tcgacgcttg gcgtacgcgt attggcgatg agcatcattg
    11341 cttacctggt ggcgctgaaa taccagcttt tccgcaacct cgcattatgg cagcaggcgc
    11401 tggtcgtcat gttgctttcg ctggtggtgg atattattgt tttctgggca gagtttttag
    11461 tgattaacgt ctctttcaga ccggaagtgt tctggagtag tgtagtcaat ggggtgctct
    11521 ggccgtggat tttcttgctg atgcgtaaag tccgtcagca gtttgcagtg caataaaggt
    11581 ttctatgact tctctgtatt tagcttccgg ttctccgcgt cgtcaggagt tacttgcgca
    11641 acttggcgtg acctttgaac gtattgttac gggcattgag gagcagcgtc agccgcagga
    11701 gagcgcgcag cagtatgttg tgcgtctggc gcgcgagaaa gcacgggcag gtgtcgtgca
    11761 aacggcgaag gatctcccgg tgctgggtgc ggatactatc gttatcctga acggagaagt
    11821 gctggagaaa ccgcgcgacg cagagcatgc ggcgcagatg ttgcgcaaat tatcgggtca
    11881 gacccatcag gtgatgacgg cagtggcgtt ggccgacagc cggcacattc tcgattgcct
    11941 ggtggtcacc gatgtgactt tcagaacgtt aacagacgaa gacatcgcgg gctatgtcgc
    12001 cagcggtgaa ccgttagata aagcaggtgc atacggtatt caggggctgg gtggctgttt
    12061 tgtcaggaag ataaatggca gctatcaagc cgtagtcggc ttaccgctgg ttgaaacgta
    12121 tgaattatta agtaatttta acgcactgcg tgagaaaagg gataaacatg acggctgaat
    12181 tgttagtaaa cgtaacgcct tcggaaacgc gagtggcgta tattgatggc ggtattctgc
    12241 aggaaattca tattgaacgt gaggcgcgac gcggaatagt aggcaatatc tacaagggtc
    12301 gtgtaagtcg tgtacttccg ggtatgcagg cggcttttgt agatattggg ctggataaag
    12361 ccgcgtttct tcatgcatct gacatcatgc cgcacaccga atgtgtggcg ggtgaagaac
    12421 aaaagcaatt cacggtgcgc gacatctcgg aactggttcg tcaggggcaa gatctgatgg
    12481 tgcaggtggt gaaagatccg cttggcacta agggggcgcg cctgaccacc gatatcacgc
    12541 tgccttctcg ctatctggtc tttatgccag gggcttctca cgttggggtt tcccaacgaa
    12601 ttgaaagcga atcagaacgt gaacgcctga aaaaagtggt cgcagagtat tgcgacgagc
    12661 agggcgggtt tatcatccgt accgcagcgg aaggggttgg cgaggctgaa ctggcctccg
    12721 atgccgctta tctgaaacgc gtctggacca aagttatgga gcgtaaaaag cgcccgcaga
    12781 cccgttatca gctgtacggc gaactggcgt tggcgcagcg tgttctgcgt gatttcgccg
    12841 atgccgaact ggaccgcatt cgcgttgact cacgcctgac ttacgaagcg ttacttgagt
    12901 tcacctcgga gtacattccc gagatgacca gcaagctgga gcattacacc ggacgccagc
    12961 cgattttcga tctctttgat gtcgaaaacg aaatccagcg agcgctggaa cgcaaagttg
    13021 aactgaaatc cggtggttat ctgattatcg accagaccga agcgatgacc accgtggaca
    13081 tcaataccgg agcgtttgtc ggtcatcgca atctggacga caccattttc aataccaata
    13141 ttgaagcgac gcaggctatc gctcgccagt tacggttgcg taatctgggc gggattatca
    13201 ttattgattt catcgatatg aataatgaag atcaccgccg ccgagtgctg cactcgctgg
    13261 agcaggcgtt gagcaaagac cgggtgaaaa ccagcgttaa tggtttttcg gcgctggggc
    13321 tggtggagat gacgcgtaaa cgcacccgcg aaagcattga gcacgtactg tgtaacgaat
    13381 gcccaacctg ccacggtcgc ggaacggtga aaaccgtgga aacggtatgc tatgaaatca
    13441 tgcgcgagat tgttcgtgta caccatgctt acgactccga ccgtttcctg gtctatgctt
    13501 ctccggcagt agccgaagcc ttgaaaggcg aagagtcaca ctcgctggcg gaagtggaaa
    13561 ttttcgttgg caaacaggtt aaagtacaaa ttgaaccgct ctataaccag gagcagtttg
    13621 acgtcgtaat gatgtaaaca gatgctgggc cgccatccgg caaagggttt ttgagtcaca
    13681 tttttagcag acaaggagtg acgggtgagg cgattgccgg ggattttact gcttactgga
    13741 gccgcgctcg ttgtgatcgc tgccctgctg gttagcggcc tgcgtattgc tttaccgcat
    13801 cttgacgcct ggcgtccgga aatcctcaac aaaatagaat ccgcgactgg catgccggta
    13861 gaagccagtc agctctcagc cagctggcag aattttggcc cgacgcttga agcgcacgac
    13921 atccgtgcag aactaaagga tggcggcgaa ttttcggtta aacgcgttac tctggcgctg
    13981 gatgtctggc agagcctgtt acatatgcgc tggcagtttc gcgacctcac tttctggcag
    14041 ctgcgctttc gcaccaacac tcctatcacc agcggtggta gcgatgacag tctggaagcc
    14101 agtcacatca gcgatctgtt tcttcgtcaa tttgaccatt tcgatctccg cgacagtgaa
    14161 gtcagtttcc tgacgccatc cggtcagcgc gccgagctgg cgatcccaca actcacctgg
    14221 ctgaacgatc cacgtcgaca ccgtgcggaa ggcctggtaa gcctctccag ccttaccgga
    14281 cagcacggcg tgatgcaggt gcgcatggat ttgcgcgatg atgaggggtt gttaagcaat
    14341 ggtcgcgtct ggctccaggc ggatgacatc gacctgaagc cgtggctcgg taaatggatg
    14401 caggacaata ttgcgctgga aacggcgcag ttctcccttg aaggctggat gacgatcgac
    14461 aaaggcgatg taaccggcgg tgacgtctgg ctgaaacagg gtggtgccag ctggttgggc
    14521 gagaaggaaa cgcatacgct gtcggtggat aatctgaccg cgcatattac gcgtgaaaat
    14581 ccgggatggc agttctctat tcccgataca cggatcacga tggacggcaa accctggccg
    14641 agcggagcat tgacgctggc ctggataccg gaacaggacg ttggcggcaa agacaataaa
    14701 cgcagtgacg aactccggat tcgcgccagt aatctggagc tggcaggcct ggagggcata
    14761 cgcccgctgg ccgcgaaact ttcacctgca ctgggtgatg tttggcgctc tacacaaccg
    14821 agcggcaaga ttaacactct ggcgctggat atcccgcttc aggcggcaga caagacccgt
    14881 tttcaggcat cgtggagcga tctggcctgg aagcaatgga aattattacc gggtgcggaa
    14941 cacttctccg ggacgctttc cggcagcgtt gaaaatggtt tgcttaccgc gtcgatgaag
    15001 caggcaaaga tgccttacga aacggtattc cgtgcgccac tggaaatcgc cgacggccag
    15061 gcaactataa gctggctgaa caatgacaaa ggtttccagc tggatgggcg taatattgac
    15121 gttaaagcca aagccgtcca tgcgcgcggc ggttttcgtt acctgcaacc tgctaacgat
    15181 gaaccctggc tgggtattct ggctggcatc agtaccgatg atggttcaca agcctggcgc
    15241 tatttcccgg aaaacttgat gggtaaagac ctggttgatt acttaagtgg cgcgattcag
    15301 ggcggtgaag cggataacgc gacgctggtt tatggtggca atccgcaact cttcccctat
    15361 aaacacaacg aaggtcagtt tgaagtgctg gtgccgctgc gcaacgcgaa gtttgccttc
    15421 cagccggact ggcctgcatt aactaacctt ggtattgaac tggactttat taacgacggt
    15481 ttatggatga aaaccgatgg cgttaatctg ggcggcgtgc gcgcgagtaa tctcaccgca
    15541 gtgatccctg actactcaaa agaaaaactg ctgattgacg ctgacattaa aggtccgggt
    15601 aaagccgttg gcccttactt tgatgagaca ccgctgaaag attctctggg tgcgaccctg
    15661 caagaactcc agctcgacgg cgatgtgaat gctcgcttac atcttgatat cccgctgaac
    15721 ggcgagctgg taaccgcgaa aggtgaagtg acgctgcgta ataacagtct gtttatcaaa
    15781 ccactcgaca gcaccctgaa aaatttgagc ggtaaattca gctttatcaa tggcgatctg
    15841 caaagtgaac cactgacagc aagctggttt aatcagccgt tgaacgtgga tttttccacc
    15901 aaagaagggg caaaagccta ccaggtcgcg gtgaatctca acggtaactg gcaaccggcg
    15961 aaaaccggcg ttctgcctgc agcggtgaac gaagcattga gtggcagcgt ggcgtgggat
    16021 ggtaaagtgg gcattgttct gccttatcat gctggcgcga cgtataacgt agagctaaac
    16081 ggcgatttga agaatgtgag cagtcactta ccttcaccgt tagccaaacc tgcgggtgaa
    16141 ccactgccgg taaacgttaa ggttgatggc aatctcaaca gctttgaatt aaccggacag
    16201 gctggtgcgg ataatcattt caatagccgc tggttgctcg gtcaaaagct gacgctcgac
    16261 cgtgctattt gggcggcaga cagtaaaacg ctcccgccgt tgccggaaca aagtggtgtt
    16321 gaactcaata tgccgccgat gaatggtgcc gagtggctgg ccctgtttca gaaaggtgcg
    16381 gcggagagtg tcggtggtgc agcgagtttc ccacaacaca taacgttacg tacgcctatg
    16441 ttgtcgctgg gaaatcagca atggaataac ctgagtattg tttcgcaacc gacggcaaat
    16501 ggcacccagg ttgaggcgca ggggcgtgaa atcaacgcca cgctggcgat gcgtaataac
    16561 gcgccgtggc tggcgaatat caaatatctt tattacaacc cgagcgtggc gaaaactcgt
    16621 ggtgattcaa cgccgtcatc acctttcccg acaacggaac gcattaactt ccgtggctgg
    16681 tcggacgccc aaatacgatg cgcagagtgc tggttctggg ggcaaaaatt cgggcgcatt
    16741 gacagtgata tcaccatttc tggcaataca ttaacgctga ccaatggact gattgatact
    16801 ggtttttcgc ggctcactgc cgatggtgaa tggattaaca atccggggaa tgaacgtacc
    16861 tcgctgaaag gaaaactgcg cgggcagaaa attgatgccg ccgcagaatt ttttggtgtc
    16921 acgacgccca tacggcagtc gtcatttaat gtggattacg atttacactg gcgtaaagca
    16981 ccgtggcagc cagatgaggc gacgttgaat ggcatcattc atactcaact gggtaaaggc
    17041 gaaattaccg aaatcaatac cggacatgcc gggcaattgc tgcgcttatt gagcgtagat
    17101 gccctgatgc gtaagctgcg ttttgatttc agagacactt ttggcgaagg gttctatttt
    17161 gactccattc gcagcaccgc gtggattaaa gacggcgtta tgcacaccga cgacacgctg
    17221 gtggatggcc tggaggcgga tatcgccatg aaagggtcgg taaatctggt acgtcgcgac
    17281 ctgaatatgg aagcggttgt cgcaccagag atttctgcga cagtgggcgt ggctgcggct
    17341 tttgcggtta accccattgt tggcgcggca gtgtttgccg ccagtaaagt gctggggccg
    17401 ctgtggagca aagtctccat tttgcgctat cacatttcgg gtccgctgga cgatccgcaa
    17461 atcaacgaag tgttgcgcca accgcgtaaa gaaaaagcgc aatgatttga cgagggcgcg
    17521 taattgcccc aatctcatag gataatcgtt gccaaaggcc aacgagccag aacataaccg
    17581 taggctggat agggcgttca cgccgcatcc ggcagccgta aaaaatcctc tactgcagta
    17641 actaacgagt agcaaaaacg atgagtctta acctggtaag tgaacaattg ctagcggcga
    17701 acggcctgaa acatcaggac ttgttcgtga tcctcggtca actggccgaa cgtcgccttg
    17761 attatggcga tctctatttt cagtcgagct atcacgaatc ctgggtttta gaagaccgca
    17821 ttattaaaga tggttcttac aacatcgatc agggcgttgg tgtgcgtgca atcagcggtg
    17881 aaaaaaccgg atttgcttac gctgaccaaa tcagcctgct ggcgctggaa cagagtgcgc
    17941 aagcggcgcg caccatcgcc cgtgatagcg gtgatggcaa agtacagacg ctgggcgcgg
    18001 tagagcatag cccgttgtat acctcggtag atccgctgca aagcatgagc cgtgaagaga
    18061 agctggatat cctgcgtcgc gtcgataagg ttgcccgcga agcggacaag cgcgtacagg
    18121 aagtgactgc cagcctcagt ggtgtctatg aattaattct ggttgcggcc accgacggca
    18181 cgctagcggc ggatgtccgt ccgctggtgc gtctttccgt gagcgttctg atcgaagaag
    18241 atggcaaacg cgaacgcggt gccagtggcg gcggcggtcg ttttggttat gagttcttcc
    18301 ttgccgatct cgacggcgaa gtccgtgcgg atgcatgggc aaaagaagca gtacgtatgg
    18361 cgctggtcaa tctttctgcc gttgctgcac cagcgggcac catgccggta gtacttggcg
    18421 caggttggcc gggcgtgctg ttgcatgaag cggtaggtca cggtctggaa ggcgacttca
    18481 accgccgtgg cacttcagta tttagtggac aggtcgggga gctggtggct tcagaactgt
    18541 gtaccgtggt tgatgacggt acgatggtcg atcgccgtgg ttcggtggcg attgatgacg
    18601 aaggtacgcc aggccagtac aacgtgctga ttgagaacgg cattctgaaa ggctacatgc
    18661 aggataaact caacgcgcgt ttgatgggga tgacgccgac tggcaacggt cgccgtgaat
    18721 cctacgccca tctgcccatg ccgcgtatga ccaacaccta tatgctgccg ggtaaatcga
    18781 ccccgcagga aattattgaa tccgttgagt acggtatcta tgcaccgaac tttggtggcg
    18841 gtcaggtgga tatcacctcc ggcaaattcg ttttctccac ttcagaagca tatctgattg
    18901 aaaacggtaa agtaacgaag ccggtgaaag gcgcaacgtt gattggttcc ggtatcgaaa
    18961 ccatgcagca gatttcgatg gttggcaacg acctgaaact ggataacggc gtgggtgtct
    19021 gcggtaaaga agggcaaagt ttgccggttg gcgtgggcca gccaacgctg aaagttgata
    19081 acctgactgt tggcggtact gcgtaataat ttcagtattt tcagaaggat aattaatcct
    19141 tcctcgtgca cgaacggtcc cctcgcccct ttggggagag ggttagggtg aggggaaccc
    19201 gttggcacag gtttgtacaa cgtaacagta caatatgaat tacttctctt tcccgcgccc
    19261 gtgcatctcc tgaaacaatt taccgacctc aacaaaataa tccgtcagcg agttgatcac
    19321 gacctgtacc ttcagcggca gcttatcttt ttcggtatat aacgcataaa ccgggcgtgg
    19381 atctgactga taacgcggca gcaggatctc cagctcccca cgattgatct cgttgatcac
    19441 ccacatcagc ggcacgtagg cgatcccggc acccgccgtt agccagcgca ccagcgtcat
    19501 cggatcatta gtcacaaatc tcccttgcgg gatcaggcga gtcgagatcc cttccggtgc
    19561 aatcagttca aattcattgt cgggccgcac gctgtattca agccatgaat ggctacttaa
    19621 atcggcgggt ttttccggta tgccgtattg tgtgagatag cttttcgcgg cgcacaccac
    19681 cattggcatc gcgccaagac ggcgggaaaa caggctggaa tcctgcaacg cgccgacgcg
    19741 gatcaccaca tccagaccgt cggcaatcag gtcgggggct ggaattccgg taaccagatt
    19801 gacgctcaaa cctgggtatt ctttcagcat tttggctgtc agcccggcga gaacattttg
    19861 tgccatagtt gaagaacagc caatgcgtag cgtcccgatg ggggtgttat tgaaggcata
    19921 cagttgctca tgaacatcct gcacttcatg aagcatacga cggcagccct ggtagtaaat
    19981 tctaccggct tcggtcaggc caatgctgcg tgtgctacgg tttaacagct ttacctgcaa
    20041 ctcatcttcc agttttgaca ccgtctgact gatggacgaa acgctcatct gtagctgtct
    20101 ggcggcggcg gtaaaagagc caaattcaac tactttggca aacaccgaca tgcgttttag
    20161 tcgttccatt attcactctg acttaaaagt gatttagatc acataatata gataacagca
    20221 taacagttac gctaatatat taaatatcaa tctacagcaa tgttgctctc gcccggcttt
    20281 ccatgccctt ctctgcggcg acagatgctg aaaataataa cgcctgctct ctctatacaa
    20341 ccaaggtcaa catgagtctg tttcccgtta tcgtggtgtt tgggctgtcc ttcccaccga
    20401 tattttttga attgctttta tcactggcga ttttctggct ggtgtgccgg gtacttgtgc
    20461 caacaggtat ctacgacttt gtctggcatc cggcgttgtt caacaccgcg ctctattgct
    20521 gcttgtttta tttgatatcg cgactgttcg tttgaggttg aagtgaaaac actaataaga
    20581 aaattctccc gtacggccat cacggtcgta ttagtcattc tggccttcat cgcaattttt
    20641 aatgcctggg tctattacac cgaatccccc tggacgcgtg acgcgcgctt tagcgctgac
    20701 gtcgttgcga tcgcgccgga cgtttctgga ctcattaccc aggtgaatgt tcataaccag
    20761 ctggtgaaaa aaggacaggt tctgttcacc atcgaccagc cgcgctatca aaaggcgctt
    20821 gaggaagcgc aagccgatgt tgcttattat caggtactgg cacaggagaa acgccaggag
    20881 gccggacgtc gtaaccgtct cggtgtgcag gcgatgtctc gcgaagagat cgaccaggcc
    20941 aacaacgtac tacaaacggt tctgcatcag ttagcgaaag cgcaggcgac ccgcgatctg
    21001 gcaaaactgg atcttgaacg cacggtgatc cgcgcgccag cagatggctg ggtgaccaac
    21061 ctcaacgtct ataccggtga gtttattact cgtggatcaa cggcggttgc gctggtgaaa
    21121 cagaactctt tctatgtact ggcctatatg gaagaaacta agctggaagg ggtgcgccct
    21181 gggtatcgtg cagagatcac gccgcttggc agtaacaaag tgctgaaagg gactgttgat
    21241 agtgttgccg ctggggtcac caacgccagc agcacgcgtg acgacaaagg gatggcgacc
    21301 atcgactcta accttgaatg ggtgcgtttg gcgcaacgtg ttccggttcg tattcgtctc
    21361 gacaaccagc aagagaacat ctggcctgcg ggcaccactg ctacagtggt ggtcactggc
    21421 aaacaagatc gcgacgaaag ccaggattcg ttcttccgta aaatggccca tcgcctgcgt
    21481 gagtttggtt aatcacgatg gatattttct ccattgctaa ccaacatatt cgctttgcgg
    21541 taaaactggc gaccgccatt gtactggcgc tgtttgttgg ctttcacttc cagctggaaa
    21601 cgccacgctg ggcggtactg acagcggcga ttgttgccgc cggtccggcc tttgctgcgg
    21661 gaggtgaacc gtattctggc gctattcgct atcgtggctt tttgcgcatc atcggcacat
    21721 ttattggctg tattgccgga ctggtgatca tcattgcgat gatccgcgca ccattattga
    21781 tgattctggt gtgctgtatc tgggccggtt tttgtacctg gatatcctcg ctggtacgaa
    21841 tagaaaactc gtatgcgtgg gggctggccg gttataccgc gctgatcatt gtgatcacca
    21901 ttcagccgga accattgctt acgccgcagt ttgccgtcga acgttgtagc gagatcgtta
    21961 tcggtattgt gtgtgcgatt atggcggatt tgctcttttc tccgcgatcg atcaaacaag
    22021 aagtggatcg agagctggaa agtttgctgg tcgcgcaata tcaattaatg caactctgta
    22081 tcaagcatgg cgatggtgaa gttgtcgata aagcctgggg cgacctggtg cgacgcacca
    22141 cggcgctaca aggcatgcgc agcaacctga atatggaatc ttcccgctgg gcgcgggcca
    22201 atcgacgttt aaaagcgatc aatacgctat cgctgacgct gattacccaa tcctgcgaaa
    22261 cttatcttat tcagaatacg cgcccggaat tgatcactga tactttccgc gaattttttg
    22321 acacgccggt agaaaccgcg caggacgtcc acaagcagct caaacgcctg cgaagagtta
    22381 tcgcctggac cggggaacgg gaaacgcctg tcaccattta tagctgggtc gcggcggcaa
    22441 cgcgttatca gcttctcaag cgcggcgtta tcagtaacac aaaaatcaac gccaccgaag
    22501 aagagatcct gcaaggcgaa ccggaagtca aagtagagtc agccgaacgt catcatgcga
    22561 tggttaactt ctggcgaacc acactttcct gcattctggg aacgcttttc tggctgtgga
    22621 cgggctggac ttccggcagt ggtgcaatgg tgatgattgc ggtcgtgacg tcactggcaa
    22681 tgcgtttgcc gaatccacgc atggtggcga tcgactttat ctacgggacg ctggccgcgc
    22741 tgccgttagg gctgctctac tttttggtga ttatccctaa tacccaacag agcatgttgc
    22801 tgctgtgtat tagcctggca gtgctgggat tcttcctcgg aatagaagta cagaaacggc
    22861 gactgggctc gatgggggcg ctggccagca ccataaatat tatcgtgctg gataacccga
    22921 tgactttcca tttcagtcag tttctcgaca gcgcattagg gcaaatcgtc ggctgtgtgc
    22981 tcgcgttcac cgttattttg ctggtgcggg ataaatcgcg cgacaggact ggacgtgtac
    23041 tgcttaatca gtttgtttct gccgctgttt ccgcgatgac taccaatgtg gcacgtcgta
    23101 aagagaacca cctcccggca ctttatcagc agctgttttt gctgatgaat aagttcccag
    23161 gggatttgcc gaaatttcgc ctggcgctga cgatgattat cgcccaccag cgcctgcgtg
    23221 atgcgccgat cccggttaac gaggatttat cggcgtttca ccgacaaatg cgccgcacag
    23281 cagaccatgt gatatctgcc cgtagcgatg ataaacgtcg tcggtacttt ggtcagttgc
    23341 tggaagaact ggaaatctac caggaaaagc tatgcatctg gcaagcgcca ccgcaggtga
    23401 cggaaccggt tcatcggctg gcggggatgc tccataagta tcaacatgcg ttgaccgata
    23461 gttaagtcaa aaccgacgcc aaaagcgtcg gttttttcat ggctatactt agcgatgcac
    23521 ggcagaactc gccgcgaaac gtgacggtgg caacagatga atatttatac ctttgatttt
    23581 gatgagattg agagtcagga ggatttttat cgtgacttta gccaaacctt tggtctggcg
    23641 aaagataagg tacgcgatct cgactcacta tgggatgtgt taatgaacga tgtcctgccg
    23701 ctaccacttg agattgaatt tgttcatctg ggagagaaaa cgcgtcgccg ttttggcgcg
    23761 ttaatattgc tgtttgatga ggcagaggaa gagctggaag ggcatttgcg ttttaatgtt
    23821 cgtcattagc gcaaaaaaaa gcccccgaac cgggggcaat atcgtcggac aagacgatga
    23881 gggtttattt gtacagttca gccgtagcgt gccaggtgtc accgctacga gcttcagtaa
    23941 tctggtaggc cgttgcgcct ttctcttccg cttttttgtt cagcatttca cgcatatcca
    24001 ttggcgaaga cgccacacca cttacggata cggtcccgat tgcttcacga ttttgtgctt
    24061 gtgcagcatc aatggagtcg gcagcgaatg caccgaaaga gagaacagaa agtacgctta
    24121 atgcagcaac agtggttttg attttcat
//
LOCUS       DBFIFM010000055        24125 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.055, whole genome shotgun sequence.
ACCESSION   DBFIFM010000055 DBFIFM010000000
VERSION     DBFIFM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.055"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            176..511
                     /locus_tag="N7C74_001914"
     CDS             176..511
                     /locus_tag="N7C74_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HGU2431791.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            770..1213
                     /gene="yjdN"
                     /locus_tag="N7C74_001915"
     CDS             770..1213
                     /gene="yjdN"
                     /locus_tag="N7C74_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HGU2431792.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1346..2134
                     /gene="phnC"
                     /locus_tag="N7C74_001916"
     CDS             1346..2134
                     /gene="phnC"
                     /locus_tag="N7C74_001916"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HGU2431793.1"
                     /translation="MQTIICVEQLSKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMAHFAYQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2159..3175
                     /gene="phnD"
                     /locus_tag="N7C74_001917"
     CDS             2159..3175
                     /gene="phnD"
                     /locus_tag="N7C74_001917"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU2431794.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFSKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAMQ"
     gene            3281..4060
                     /gene="phnE"
                     /locus_tag="N7C74_001918"
     CDS             3281..4060
                     /gene="phnE"
                     /locus_tag="N7C74_001918"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HGU2431795.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMLVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4081..4806
                     /gene="phnF"
                     /locus_tag="N7C74_001919"
     CDS             4081..4806
                     /gene="phnF"
                     /locus_tag="N7C74_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HGU2431796.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4807..5259
                     /gene="phnG"
                     /locus_tag="N7C74_001920"
     CDS             4807..5259
                     /gene="phnG"
                     /locus_tag="N7C74_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HGU2431797.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITANYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5256..5840
                     /gene="phnH"
                     /locus_tag="N7C74_001921"
     CDS             5256..5840
                     /gene="phnH"
                     /locus_tag="N7C74_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HGU2431798.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     GEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAVPRTTHVEVC"
     gene            5840..6904
                     /gene="phnI"
                     /locus_tag="N7C74_001922"
     CDS             5840..6904
                     /gene="phnI"
                     /locus_tag="N7C74_001922"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HGU2431799.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDESPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6897..7742
                     /gene="phnJ"
                     /locus_tag="N7C74_001923"
     CDS             6897..7742
                     /gene="phnJ"
                     /locus_tag="N7C74_001923"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HGU2431800.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     VIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7739..8497
                     /gene="phnK"
                     /locus_tag="N7C74_001924"
     CDS             7739..8497
                     /gene="phnK"
                     /locus_tag="N7C74_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HGU2431801.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8608..9288
                     /gene="phnL"
                     /locus_tag="N7C74_001925"
     CDS             8608..9288
                     /gene="phnL"
                     /locus_tag="N7C74_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HGU2431802.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWRLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9285..10421
                     /gene="phnM"
                     /locus_tag="N7C74_001926"
     CDS             9285..10421
                     /gene="phnM"
                     /locus_tag="N7C74_001926"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HGU2431803.1"
                     /translation="MIINNVKLVLENEVVNGSLEMQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHNTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAM
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10421..10978
                     /gene="phnN"
                     /locus_tag="N7C74_001927"
     CDS             10421..10978
                     /gene="phnN"
                     /locus_tag="N7C74_001927"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HGU2431804.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10965..11400
                     /gene="phnO"
                     /locus_tag="N7C74_001928"
                     /pseudo
     CDS             10965..11400
                     /gene="phnO"
                     /locus_tag="N7C74_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
     gene            11402..12160
                     /gene="phnP"
                     /locus_tag="N7C74_001929"
     CDS             11402..12160
                     /gene="phnP"
                     /locus_tag="N7C74_001929"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HGU2431805.1"
                     /translation="MSLTLTLTGSGGAQGVPAWGCECAACARARRSPHYRRQPCSGVV
                     KFNEAITLIDAGRHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     VLTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(12237..12566)
                     /gene="yjdP"
                     /locus_tag="N7C74_001930"
     CDS             complement(12237..12566)
                     /gene="yjdP"
                     /locus_tag="N7C74_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HGU2431806.1"
                     /translation="MKRHSTLFLFTLLTLTTVPAQADIIDDTIGNIQQAINDASNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQHQLNQERRQLEDEERR
                     MEDEYGQ"
     gene            complement(12635..14905)
                     /locus_tag="N7C74_001931"
     CDS             complement(12635..14905)
                     /locus_tag="N7C74_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU2431807.1"
                     /translation="MAHPGRHFFASARGRLLLLNLLVVAVTLMVSGVAVMGFRHASQM
                     QELVQQQTVDDMTGSLNLARDTANVATAAVRLSQVVGALEYKGEAERLQETQRALKSS
                     LAQLANAPLAQQEAGLVTRIITRSNELQTSVGGMLERGQRRHLERNALLSSLYQNLSY
                     LRHLQKVTHAQDDILLNEMNRLIVAAIATPAPQAIIHQLVGVMSALPTHSDTPLVNTL
                     LNDFNDFNDEMRKLAPLSAALEQSDLAISWYMFHIKALVAILNGDINRYAEQVATLSG
                     QRVAQSYQDLRSGERVIMIFALLAVVITAFAGWYIYRNLGTNLTAISRAMSRLAHGEA
                     DVTFPALQRRDELGELARAFNVFARNTASLERTSRLLKEKTTQMEIAQTERQGLEEAL
                     LHSQKLKAVGQLTGGLAHDFNNLLAVIIGSLELVNPDSPDAPRIQRALQAAERGALLT
                     QRLLAFSRKQSLHPQAVELKTLLEELSELMRHSLPATLALEIEAQSPAWPAWIDISQL
                     ENAIINLVMNARDAMEGQTGIIKIRTWNQRVTRSSGQRQDMVALEVIDHGCGMSQAVK
                     SQVFEPFFTTKQTGSGSGLGLSMVYGFVRQSGGRVEIESAPGQGTTVRLQLPRALTAA
                     KNLSPAAVEQASVSGDKLVLVLEDEAAVRQTICEQLHLLGYLTLEASSGEQALDLLAA
                     SAEIDIFISDLMLPGGMSGAEVVNAARKLYPHLTLLLISGQDLRPSHNPALPDVALLR
                     KPFTRAQLAQALGQEN"
     gene            15019..16539
                     /locus_tag="N7C74_001932"
     CDS             15019..16539
                     /locus_tag="N7C74_001932"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGU2431808.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLSVAENI
                     FLGSELSHGGLVQRKEMLVQAQKVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSSL
                     LVRSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAAENITMATLERDARWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPARGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVYVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            16536..17510
                     /locus_tag="N7C74_001933"
     CDS             16536..17510
                     /locus_tag="N7C74_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HGU2431809.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFITPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVVCWFILRRTTLGVHIYAVGSNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSASRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAMIIIAVLIDKYRTRHHQSA"
     gene            17529..18470
                     /locus_tag="N7C74_001934"
     CDS             17529..18470
                     /locus_tag="N7C74_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU2431810.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKELKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNQNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     IGYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            18525..19208
                     /locus_tag="N7C74_001935"
     CDS             18525..19208
                     /locus_tag="N7C74_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HGU2431811.1"
                     /translation="MKRSAINDILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     EVFDLKLGWDVTAFGRNNFAAHGLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTADSDITVVIDGCRQKHTAGSQLRLSPG
                     ESICLPPGLYHSFWAEAGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFR"
     gene            19223..20083
                     /locus_tag="N7C74_001936"
     CDS             19223..20083
                     /locus_tag="N7C74_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HGU2431812.1"
                     /translation="MPLISLAEGLAHAREHHYSLGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARHDIPVVLNLDHGLHFESVVQALRLGFSSVM
                     FDGSTLGYEENVRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGISDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            20080..21081
                     /locus_tag="N7C74_001937"
     CDS             20080..21081
                     /locus_tag="N7C74_001937"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HGU2431813.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLPLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHITHIAAAARELLDAGVRQRVVIHYPEGAWGETPEQGGVWIPSQKLEQREIIGS
                     VGAGDAFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(21078..21785)
                     /locus_tag="N7C74_001938"
     CDS             complement(21078..21785)
                     /locus_tag="N7C74_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGU2431814.1"
                     /translation="MKPVVLVVDDDTAICALLQDVLSEHVFTVSVCHTGQEAILRIEG
                     DPDIALVVLDMMLPDTNGLRVLQQIQKLRPTLPVVMLTGMGSESDVVVGLEMGADDYI
                     CKPFTPRVVVARLKAVLRRVGALAVNDEKSAGLSFNGWYLDTMRCQLHNPLQQHVELT
                     QGEYGLLLALAQNARRVLSREQLLAFTHSDSVEVFDRTIDVLIMRLRRKIELNPHQPM
                     LIKTLRGLGYVFAADVH"
     gene            22076..24061
                     /gene="yjcS"
                     /locus_tag="N7C74_001939"
     CDS             22076..24061
                     /gene="yjcS"
                     /locus_tag="N7C74_001939"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HGU2431815.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKVYYRADDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTITFIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVKALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMAVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         5377 a         7025 c         6753 g         4970 t
ORIGIN      
        1 aaatggaggc aaaaagatta ttttcttttt gcgtttcctt tcaaatgaaa acgatcgtcg
       61 tctaaaatca gcagtacccc cgacaaactc agggattttg tgtataattg cggccttttt
      121 cggcaatctg ccgttttttg gcgcttttgc cctgctgact tttgaggaaa tccacatgtc
      181 attaccacac tgcccaaaat gcaactccga atacacttac gaagataacg gcatgtacat
      241 ctgcccggaa tgtgcctacg aatggaacga cgcagaacct gcacaggaaa gcgacgagct
      301 gatcgttaaa gatgctaacg gcaatctgct ggctgacggc gacagcgtta ccatcattaa
      361 agatctgaag gtgaaaggta gctcttcgat gctgaaaatt ggcaccaaag tgaaaaacat
      421 ccgcctggtt gaaggcgacc ataacatcga ttgcaaaatc gacggttttg gtccgatgaa
      481 actgaaatct gagtttgtga aaaagaactg attgtattgt gatcggtaag ccggataagg
      541 cgtttacgcc gcatccggca acagtgccag atgcctgatg cgacgctcgc gcgtcttatc
      601 aggcctacaa attcccgcac cctccgtagg ccggataagg cgtttacgcc gcatccggca
      661 acagtgccga ctgcctgatg cgacgctcgc gcgtcttatc aggcttccct ctcatctgta
      721 taaatttcga actacactta actggcttct cttaactgag gtcaccatca tgccgttaag
      781 tccctacctc tcttttgccg gtaactgctc cgacgcgatt gcctattatc aacgtacgtt
      841 gggcgctgaa ctgctctata aaatcagctt cggcgaaatg ccaaaatcag cgcaggacag
      901 cgccgagaac tgcccttccg gaatgcaatt tcccgatacc gccatcgccc acgccaatgt
      961 gcgcattgcc ggaagcgata tcatgatgag cgatgctata ccgtcaggaa aagccagcta
     1021 ctccggtttt acgctggtgc tcgattcgca acaggtcgaa gaaggaaaac gctggtttga
     1081 taaccttgcc gctaacggaa aaatcgaaat ggcctggcag gaaactttct gggcgcatgg
     1141 ctttggcaaa gtcaccgata aatttggcgt accgtggatg attaatgtcg tcaaacaaca
     1201 accaacgcaa taatccgccg ggaggcccgc cctcccgcac tgtcatcgaa ttcccgttaa
     1261 ctcttcatct gttagtcact tttaattaac caaatcgtca caataatccg ccacgatgga
     1321 gccacttttt tagggaggct gcatcatgca aacgattatc tgcgtcgaac aactgagcaa
     1381 aaccttcaac cagcatcagg cgctgcacgc ggttgatctg aatatccatc atggtgaaat
     1441 ggtggctctg cttgggccgt ccggttccgg caaatccacc cttttacgtc acttaagcgg
     1501 tttgattacc ggcgataaat ccgccggtag ccatatcgag ctgctgggcc gcactgtcca
     1561 gcgcgaaggc cgcctggccc gcgatatccg caaaagccgc gccaacaccg gctacatctt
     1621 ccaacaattc aacctggtga atcgcctgag cgtactggag aacgtgctga ttggcgcgct
     1681 cggcagcacg ccgttctggc gcacctgttt tagttggttc acccgcgagc agaaacagcg
     1741 cgcattacag gcgctgaccc gcgtcggtat ggcgcacttt gcgtatcagc gtgtttccac
     1801 gctctccggc ggccagcagc agcgtgtggc gattgcccgc gcgctgatgc agcaggcgaa
     1861 agtgattctc gctgacgaac cgatcgcctc gctggacccg gaatcggcgc gcatcgtgat
     1921 ggacaccctg cgcgacatca accagaacga cggcatcacc gtggtcgtca cgctgcatca
     1981 ggtggattac gccctgcgct actgcgaacg catcgtcgcc ctgcgccagg ggcacgtctt
     2041 ctacgacggc agcagccaac agtttgataa cgaacgtttt gaccatctct accgcagcat
     2101 caaccgcgtc gaagagaacg cgaaagctgc ctgacatccc catcattgag gaaaacgaat
     2161 gaacgctaag ataattgcct cgctggcctt caccagcatg ttcagcctca gcaccctgtt
     2221 aagcccggca cacgccgaag agcaggaaaa ggcgctgaat ttcggcatta tttcaacgga
     2281 atcacagcaa aacctgaaac cgcaatggac gccgttttta caggatatgg agaagaagct
     2341 gggcgtgaag gtcaacgcct tctttgcccc ggactatgcg ggcattatcc agggaatgcg
     2401 tttcaacaaa gtggatatcg cctggtacgg caacctgtcg gcgatggaag cagtggatcg
     2461 cgccaacggc caagtcttcg ctcagaccgt cgcggcggat ggatcgccgg gttactggag
     2521 cgtgttgatc gtcaacaaag acagcccgat caacaacctg aacgatctgc tggcgaagcg
     2581 gaaagatctc accttcggta atggcgatcc taactccacc tctggcttcc tcgtccccgg
     2641 ctactacgtc ttctccaaaa acaatatctc cgccagtgac ttcaagcgca ccgtcaacgc
     2701 cgggcatgaa accaacgcgc tggccgtcgc caacaagcag gtggatgtgg cgaccaacaa
     2761 caccgaaaac ctcgacaagc tgaaaacctc cgcgccggag aagctgaaag aactgaaagt
     2821 gatctggaag tcaccgctga tcccaggcga tccgatcgtc tggcgtaaaa acctttccga
     2881 aaccaccaaa gacaagatct acgacttctt tatgaattac ggcaagacgc cggaagagaa
     2941 agcggtgctg gaacgcctgg gctgggcgcc gttccgcgcc tccagcgacc tgcaactggt
     3001 gccgattcgc cagctcgcac tgtttaaaga gatgcaaagc gtgaaaggca ataaagggct
     3061 gaatgagcag gacaagctgg caaaaaccac cgagattcaa gcgcagctgg atgacctgga
     3121 ccgcctgaac aacgcgttaa gcgcgatgag ttcggtaagt aaagcgatgc agtaaatcgt
     3181 aggtcggata agacgccccg gtgtcgcatc cgacaatgtg caggcgttga tgccggatgc
     3241 ggtgcaagca ccttatccgg cctacagacc ggagccaaac atgcaaacca tcaccatcgc
     3301 cccacccaag cgcagctggt tctcgcttct gagctgggcc gttgtactcg ccgtgttggt
     3361 cgtctcgtgg cagggcgcgg aaatggcccc gctcacgctg atcaaagacg gcggcaacat
     3421 ggcgaccttc gccgccgact tcttcccgcc cgatttcagc cagtggcagg attacctcac
     3481 cgaaatgctc gtcacgctgc aaatcgccgt ctggggcacc gcgctggcgg tggttctctc
     3541 catccccttc ggcctgatga gcgccgaaaa cctggtgccg tggtgggttt accagcccgt
     3601 gcgccgcctg atggacgcct gccgcgccat taacgaaatg gtcttcgcca tgctgttcgt
     3661 ggtcgccgtc ggcctcggcc cattcgctgg tgtgctggcg ctgtttatcc acaccaccgg
     3721 tgtgctctcc aaactgcttt ccgaagcggt ggaagcgatt gaacccggcc cggtggaagg
     3781 cattcgcgcc accggcgcca acaagctcga agagatcctc tacggcgtgc tgccgcaggt
     3841 gatgccgctg ctgatctcct actccctcta tcgcttcgaa tccaacgtcc gctcggcgac
     3901 cgtcgtcggc atggtcggcg cgggcgggat cggcgtcacg ctgtgggaag cgattcgcgg
     3961 tttccagttc cagcaaacct gcgccctgat ggtgctgatt atcgtcacgg tcagcctgct
     4021 ggatttcctc tctcaacggt tgcgtaagca ctttatctga ttagcgaggc attgatagct
     4081 atgcacttgt ctacacatcc gaccagctac ccaacacgct atcaggagat agccgcaaaa
     4141 cttgagcagg agcttcgtca acactaccgc tgcggcgact atcttcccgc cgagcagcag
     4201 ctggcggcgc gctttgaggt aaatcgccac accctgcgcc gcgccatcga ccaactggtg
     4261 gaaaaaggct gggtacagcg ccgtcagggc gtcggcgtgc tggtgctgat gcgcccgttc
     4321 gattacccgc tcaacgccca ggcgcgtttt agccagaatc tgctggatca gggcagccat
     4381 cccaccagcg aaaaactgct gtctgtgctg cgcccggcct ccggtcacgt cgctgacgcg
     4441 ctggggataa ccgaggggga gaacgtcatc catctgcgca ccctgcgccg ggtcaacggc
     4501 atcgcgctct gtttaatcga ccactacttc gcggacctca ccctctggcc gacgctgcaa
     4561 cgcttcgaca gcggctcgct gcacgatttt ctgcgcgagc aaacggggat tgcactgcgc
     4621 cgcagccaga cgcggatcag cgcccgccgc gcccaggcca aagagtgcca gcgtcttgaa
     4681 attccgaata tgtccccgct gctgtgcgtg cgcaccctta accaccgtga cggtgaaagc
     4741 agcccggcgg agtactccgt cagcctgacg cgcgccgaca tgattgaatt cactatggag
     4801 cactgaatgc acgcagatac cgcgacccgc cagcactgga tgtccgtact ggcacacagc
     4861 caaccggcag aactggcagc ccgcctgaac gcgctgaaca tcaccgccaa ctatgaggtg
     4921 atccgcgccg ctgaaactgg cctggtacag attcaggcgc ggatgggcgg caccggcgaa
     4981 cgcttttttg ccggcgacgc cacgctgacc cgcgccgccg tacgtctgac tgacggcacc
     5041 ctcggctaca gctgggtgct ggggcgcgac aaacagcacg ccgaacgctg cgcgctgatt
     5101 gacgcgctga tgcagcaaac ccgccatttt caaaacttat cagaaacgct tattgccccg
     5161 ctggacgctg accgtatggc acgcattgcc gcacgccagg ccgaagtgaa cgccagccgg
     5221 gtcgacttct ttacgatggt tcgcggagac aacgcatgac cctggaaacc gcttttatgc
     5281 ttcccgtgca ggatgcccag cacagttttc gtcgcctgtt aaaggccatg agcgagccgg
     5341 gcgtgattgt cgccctgcat cagctcaaac gcggctggca gccgctgaat atcgccacca
     5401 ccagcgtgct gctgacgctg gccgataacg acacgccggt gtggcttgcc gcgccattga
     5461 gcaacgatat cgtcagccag agcctgcgtt ttcataccaa cgcgccgctg gtcagccagc
     5521 cggaacaggc gaccttcgcg gtaacggatg aggcgatttc cggcgaacag cttaacgccc
     5581 tttccaccgg caccgccgtt gcgccggaag cgggcgcaac gctgatttta caggtcgcca
     5641 gcctgagcgg cgggcgcatg ctgcgtctca ccggcgcggg tattgccgaa gaacgaatga
     5701 tcgccccgca gctgccggag tgcattctgc acgaactcac cgagcgcccg cacccgttcc
     5761 cgctcggcat cgacctgatc ctgacctgcg gcgagcgcct gctggctgtt ccgcgaacca
     5821 ctcatgtgga ggtgtgctga tgtacgttgc cgtgaaaggg ggcgagaagg cgatcgacgc
     5881 cgcccacgcc ctgcaagaga gccgacgccg gggcgatacc gatttgcctg aactgagcgt
     5941 cgcccagatt gaacagcagc ttaatcttgc ggtggatcgc gtgatgaccg aaggcggcat
     6001 tgccgaccgc gagctggcgg cgctggcgct gaaacaggcc agcggcgata acgtcgaagc
     6061 aattttcctg ctgcgcgcct accgcaccac gctggcgaag ctcgcggtaa gcgagccgct
     6121 cgacaccacg gagatgcgtc tcgaacggcg tatttcagcg gtttataaag atattcccgg
     6181 cggccagctg cttggcccaa cctacgacta cacccatcgc ctgctcgatt tcaccctgct
     6241 ggcaaacggc gaagcgccga cgctgaccac cgccgacagc gaacagcagc cgtcgccgca
     6301 cgttttcagc ctgctggcgc gtcaggggct ggcgaagttt gaagaggata gcggcgcaca
     6361 gccggatgac atcacccgca cgccaccggt ttacccctgc tcacgttctt cccgcctgca
     6421 acagctcatg cgcggcgacg aaggctattt gctggcgctg gcctactcca cccaacgcgg
     6481 ttacgggcgc aatcacccgt tcgcgggtga aatccgcagt ggttacatcg acgtgtcgat
     6541 tgtgcccgaa gagctgggat ttgcggtgaa cgtcggcgaa ctgctgatga ctgagtgtga
     6601 aatggtcaac ggttttatcg acccaccgga tgaatctccg cacttcacgc gcggctacgg
     6661 gctggtgttc ggcatgagcg agcgcaaagc gatggcgatg gcgctggtcg accgcgctct
     6721 gcaagccccg gagtacggcg agcacgcgac aggcccggcg caggatgaag agttcgtgct
     6781 ggcacatgcc gacaacgtcg aagccgcagg ctttgtctcg cacctcaaac tcccgcacta
     6841 cgtcgatttc caggccgaac tggagctact caaacgtctg caacaggagc agaaccatgg
     6901 ctaatctgag cggctacaac tttgcctacc tcgacgagca gaccaaacgc atgatccgcc
     6961 gcgccatctt aaaagcggtg gcgatccccg gttatcaggt gccgtttggc gggcgcgaga
     7021 tgccaatgcc gtacggctgg ggaaccggcg gcattcagct taccgccagc gtgattggcg
     7081 aaagcgacgt gctgaaggtg attgaccagg gcgcggatga caccaccaac gccgtgtcga
     7141 ttcgcaactt ctttaagcgc gtgaccgggg taaacaccac cgagcgcacg gacgatgcaa
     7201 cggtcatcca gacgcgtcac cgcatccccg aaacgccgct gaccgaagat cagatcatta
     7261 ttttccaggt gccgatcccc gaaccgctgc gctttatcga gccgcgcgaa acggaaaccc
     7321 gcaccatgca cgcgctggag gaatacggcg tgatgcaggt gaagctgtat gaagatatcg
     7381 cccgcttcgg tcatatcgcc accacctacg cctatccggt gaaggtgaac gggcgctacg
     7441 taatggaccc gtcgccgatc ccgaaattcg ataacccgaa aatggacatg atgcccgccc
     7501 tgcaactctt cggcgccggg cgtgagaagc gcatctatgc ggtgccgccg tttacccgcg
     7561 tggaaagcct cgatttcgac gatcacccgt tcaccgttca gcagtgggat gagccatgcg
     7621 ccatctgcgg atcgacccac agctatcttg atgaagtggt gctggatgac gccggaaacc
     7681 gcatgtttgt ctgctccgat accgattatt gccgccaaca gagcgaggca aaaagccaat
     7741 gaatcaaccg ttactttcgg tcaataacct gacccacctt tacgcgccgg gcaaaggctt
     7801 tagcgatgtc tcttttgatt tatggccggg ggaagtgctg ggcattgtcg gggaatccgg
     7861 ctccgggaag accacgctgc tgaaatcaat ttccgcgcgt ctgacgccgc agcaggggga
     7921 gattcactat gaaaaccgtt ccctctatgg catgagcgag gccgaccgcc gtcgcctgct
     7981 gcgcaccgaa tggggcgtag tgcatcagca tccgctcgac ggcctgcgcc gccaggtctc
     8041 ggcaggcggc aatatcggcg agcggctgat ggcgaccggg gcgcgtcatt acggcgatat
     8101 tcgcgccacc gcgcagaagt ggctggaaga ggtggaaatt cccgccaacc ggatcgacga
     8161 cctgccgacc accttttccg gcggtatgca gcagcgtttg cagattgccc gcaacctggt
     8221 gacgcatccg aagctggtgt ttatggatga accgaccggc gggctggatg tgtcggtgca
     8281 ggcccgcctg ctcgacctgc tgcgcggcct ggtggtggag ctgaacctcg cggtagtgat
     8341 tgtcacccac gatttaggcg tcgcccgcct gctggcggac cgtttgctgg tgatgaagca
     8401 ggggcaagtg gtggagagtg ggttaaccga ccgcgtgctc gacgacccgc atcatccgta
     8461 tacacagctg ctggtgtcat cggttttgca gaattgagcc ggtgccggat gcggcgtaaa
     8521 cgccttatcc ggcctacaaa tgcgctcccc gtaggtcgga taagacgcat cagcgtcgca
     8581 tccgacaccc gaaccacgag gcgaaaaatg attaacgtac aaaacgtcag taaaaccttc
     8641 atcctgcacc agcaaaacgg cgtgcgcttg cccgtcctca accgcgcctc gctcaccgtc
     8701 aacgcgggcg aatgcgtggt gctccacggc cactccggca gcggcaaatc aaccctgctg
     8761 cgctcgctgt acgccaacta tctgcccgat gaagggcaaa tccagatcaa acacggtgac
     8821 gagtgggtag acctggtcac tgcgccagcg cgcaaagtgg tggaaatccg caaaaccacc
     8881 gttggctggg tgagccagtt tctgcgcgtt atcccgcgca tttcggcgct ggaagtagta
     8941 atgcagccgc tgctcgatac cggcgtaccg cgtgaagcct gcgccgctaa agccgcgcgt
     9001 ctgctcaccc gtctgaacgt gccggaacgc ctgtggcgcc ttgcgccatc gaccttctcc
     9061 ggcggcgaac agcagcgcgt caacatcgcc cgtggcttta tcgtcgacta ccccattctg
     9121 ctgctcgacg aacctaccgc ctcgctggac gccaaaaaca gcgccgccgt agtcgaactg
     9181 atccgagaag ccaaagcccg cggcgcagcc atcgtcggca ttttccatga cgaagccgta
     9241 cgtaatgacg tcgccgaccg cctgcaccca atgggagcct cttcatgatt atcaataacg
     9301 tcaaactggt gctggaaaac gaagtggtaa acggttcgct ggagatgcag gacggcgaaa
     9361 tccgcgcctt tgccgaaagc cagagccgcc tgccggaggc gatggacggc gaaggcggct
     9421 ggctgctgcc ggggctgatt gagctgcata cagacaacct cgataaattc ttcaccccgc
     9481 gcccgaaagt tgactggccc gcccactcgg cgatgagcag ccatgacgcg ctgatggtgg
     9541 cgagcggcat caccaccgta ctggacgccg tggcgattgg cgacgtgcga gacggcggcg
     9601 atcggctgga gaatctggag aagatgatca acgccatcga agagacgcag aaacgcggcg
     9661 tcaaccgcgc cgagcaccgc ctgcacctgc gctgcgagct gccgcataac accacgctgc
     9721 cgctgtttga aaagctggtg cagcgcgagc cggtgacgct ggtgtcgctg atggatcact
     9781 cgccgggcca gcgccagttc gccaaccgcg agaagtatcg cgaatattat cagggcaaat
     9841 actcgctcac cgatgcgcag atgcagcagt atgaagagga acagctggcg cttgccgcac
     9901 gctggtcgca gccgaatcgc gaatccatcg ccgcaatgtg ccgcgcgcga caaattgcgc
     9961 tcgccagcca cgatgacgca acgcacgccc acgttgccga atctcaccag cttggcagcg
    10021 tgatcgccga atttcccacc acgtttgaag cagcggaagc ctcgcgcaag catggcatga
    10081 acgtgctgat gggcgcgcca aatatcgtgc gtggcggttc gcactccggc aacgtggcgg
    10141 ccagtgaact ggcacagctt ggcctgctgg atatcctctc ttccgactac taccccgcca
    10201 gcctgctgga tgcggcgttc cgcgtcgccg atgaccagag caaccgcttt acgctgccgc
    10261 aggcggtgaa gctggtgacc aaaaatccgg cgcaggcgct taatctccag gatcgcgggg
    10321 tgattggcga gggcaaacgc gccgacctgg tgctggcgca tcgcaagggc aatcacattc
    10381 atatcgacca cgtctggcgt cagggtaaaa gggtgttctg atgatgggaa aactgatttg
    10441 gttaatgggg ccgtccggct ccgggaaaga tagcctgctg gcggaactcc gcctgcggga
    10501 acaaactcag ctactggtgg cgcatcgcta catcacgcgc gacgccagcg ccggaagcga
    10561 aaaccatatc gccctgagcg agcaggaatt ttttacccgc gcggggcaaa atctgctggc
    10621 cttaagctgg cacgctaacg gtctgtatta tggcgtcggc gtcgagattg acctctggct
    10681 gcacgctgga ttcgacgtgc tggtcaacgg ctcacgcgcc catctgccgc aggcgcgggc
    10741 gcgctatcaa tcggcgctgc tgcccatttg cttacaggtt tcgccagaga tcctgcgcca
    10801 gcgcctggaa aaccgtggcc gcgaaaacgc cagtgaaatt aacgcccggc tggcgcgcgc
    10861 cgcccgctat actccacagg attgccatac gctcaataat gacggcagcc tgcgccagtc
    10921 ggtcgacacg ctgctgacgc taatccatca gaaggagaaa caccatgcct gcttgtgagc
    10981 tttcgcccgg ccacgcagta cgacaccgac gcggtttacg cgttgatttg cgagctaaaa
    11041 caggcggagt ttgaccacca cgcgtttcgc gtgggcttta acgccaacct gcgcgacccg
    11101 aacatgcgct accatctggc gctgctggat ggcgaagttg tcggtatgat cggcctgcat
    11161 ttgcagtttc atctgcatca tgtcaactgg atcggcgaaa ttcaggagtt ggtggtaatg
    11221 ccgcaggcgc gcggtctgaa cgtcggcagt aagttactgg cgtgggcaga agaagaagcc
    11281 cgccaggccg gggccgaaat gaccgaactt tcgaccaacg tgaagcgcca cgacgcgcac
    11341 cgtttctatc tgcgcgaagg ctacgagcag agccacttcc gcttcaccaa ggtgctgtaa
    11401 catgagcctg accctcacgc tcaccggctc cggcggcgca cagggcgttc cggcatgggg
    11461 ctgcgagtgt gcggcctgcg ccagagcgcg gcgctcgccg cactatcgcc gccagccatg
    11521 cagcggcgta gtgaagttta acgaggcgat caccctgatc gacgccgggc ggcacgatct
    11581 cgccgatcgc tggtcgcccg gatcgttcca gcagtttttg ctgacgcact accatatgga
    11641 tcacgtgcag gggctattcc cgctgcgctg gggcgtgggc gatccgatcc cggtttacgg
    11701 cccaccggat gaacagggct gcgacgatct gtttaaacat ccgggcctgc ttgatttcag
    11761 ccacacggtg gaaccgtttg tggtgtttga tttgcagggg ttacaggtca cacccctgcc
    11821 gctcaaccac tcaaaactga ccttcggtta tctgctggaa acggcgcaca gccgggtggc
    11881 gtggctgtct gacaccgctg gcttgccgga aaaaacgctg aaatttttac gcaataatca
    11941 gccgcaggta atggtgattg attgcagcca cccgccgcgc gaggatgcac cgcgtaatca
    12001 ctgtgattta aataccgtgc ttgcgctgaa tcaggttatc cgctcgccgc gcgtggttct
    12061 gacccatatc agccaccagt ttgatgcgtg gctgatggaa aacgcactgc cgtcggggtt
    12121 tgaggcgggg tttgatggga tggagattgg ggtggcgtga tgagagggaa tgtgcgtgct
    12181 ggccccctca ccctaaccct ctccccagag gggcgatgcc tgccccatac ccaacatcat
    12241 tgcccatact catcttccat tctccgctct tcatcttcta gctgccgacg ctcctgatta
    12301 agctggtgct ggcgatcgtc cagctgcctg cgacgatctt caaactggcg acggcggtcg
    12361 tcatattgtc tgcgccgatc gtcgctcact tcacgctgcc agccgtcgtc gcgcgaatct
    12421 tcataatctc gcccacggtc agggttagaa gcgtcgttaa tcgcctgctg aatattgcca
    12481 atggtgtcat cgataatatc ggcctgggcc ggtacggtgg tcagcgtgag cagggtgaaa
    12541 agaaaaagcg tggagtggcg tttcataaac tcggcctcgc aacaaagtta atacaaggta
    12601 ggcaaaggtg cctgttacga gtagaggagg attctcaatt ctcttgcccc agcgcctgcg
    12661 ccaactgcgc acgggtaaag ggtttgcgca acagcgccac gtctggcagc gcggggttat
    12721 ggctggggcg taaatcctga ccgctgatga gtagcagcgt caggtgcggg taaagtttgc
    12781 gggccgcatt gaccacttct gcgccgctca tgccgccggg gagcattaaa tcgctgataa
    12841 aaatatcgat ttctgctgat gccgccagca gatccagcgc ctgttcgccg ctgcttgctt
    12901 ccagcgtcaa atagcccagc aggtgcaact gttcgcagat ggtctggcgc accgcggctt
    12961 catcttccag cactaacacc agtttatcgc cgctgacaga agcctgttct actgccgctg
    13021 gcgataggtt tttcgccgcc gtcagcgcgc ggggaagctg taatctgacc gttgtcccct
    13081 gccccggcgc gctttcaatt tcaacgcgcc caccggactg gcgcacaaaa ccatagacca
    13141 tcgacaaacc cagcccgctg ccgctgcccg tctgtttggt ggtaaagaat ggctcaaata
    13201 cctggctttt caccgcctgc gacataccgc agccatgatc gatgacctcc agtgccacca
    13261 tgtcctgacg ctgcccactg ctgcgagtaa cgcgctgatt ccaggtacga attttgatga
    13321 ttcccgtctg cccttccatc gcgtcacggg cgttcatcac caggttgatg atggcatttt
    13381 ccagttggct gatgtcaatc cacgcgggcc acgccggaga ctgcgcttca atctccagcg
    13441 ccagcgttgc cggtaaggag tggcgcatta attcgcttaa ctcttccagc aatgttttca
    13501 gttcaacggc ttgcggatgt aaggactgct tgcgggaaaa cgccagcagt cgttgtgtca
    13561 gtagcgcccc acgttcggca gcttgcagcg cccgctgtat tcgcggcgcg tctggcgagt
    13621 ccggattcac cagctccagg ctgccaataa tcaccgccag cagattatta aaatcatggg
    13681 ccagcccgcc cgtcagttgc ccgacagctt tcagtttctg gctgtgtaat agcgcctctt
    13741 ccaacccctg ccgctcagtt tgggcaatct ccatctgcgt ggtcttctct tttagcagcc
    13801 gtgaggttcg ttcgagtgac gcggtattgc gggcaaaaac gttaaaggcg cgcgccagtt
    13861 cacccaactc atcacgccgc tgtaacgccg gaaaagtgac gtccgcttcg ccgtgcgcca
    13921 ggcggctcat ggcgcgggaa atcgccgtca ggttagtgcc gaggttgcga tagatatacc
    13981 agccggcaaa cgcggtgatc accaccgcta acagggcgaa aatcatgatc accctctcgc
    14041 cggagcgcaa atcctggtaa ctttgcgcca cacgctgacc ggagagcgtc gccacctgtt
    14101 cggcgtaacg gttgatgtcg ccattgagga tcgccaccag cgctttgatg tgaaacatat
    14161 accagctgat tgccagatcg ctttgctcca gagcggcaga aagcggcgcg agtttacgca
    14221 tttcatcatt gaaatcattg aaatcattga gtaacgtatt aaccaatggc gtatcgctgt
    14281 gggtcggcaa cgcagacatt accccgacca gttgatgaat aatcgcctgc ggcgcggggg
    14341 tggcaatagc ggcaacaatc agtcggttca tctcgttaag caaaatatca tcttgcgcgt
    14401 gagtgacttt ttgcagatgc cgcagataac ttaagttttg atacagcgaa cttaacagcg
    14461 cgttgcgctc cagatgccgc ctttgcccgc gctccagcat accgccgacg ctggtttgca
    14521 gttcgttgct acgcgtgatg attcgcgtca ccagcccggc ttcctgctgc gctaaaggcg
    14581 cgttcgccag ttgtgcaagc gagcttttta atgcccgctg tgtctcctgc aaccgctcgg
    14641 cttcgccttt atattccagc gctcccacca cctgcgacaa tcgcaccgcc gccgttgcca
    14701 cgtttgccgt gtcgcgcgcc agattcaggc tgccggtcat atcatccacc gtttgctgct
    14761 gcaccagctc ctgcatctgg ctggcatgac gaaagcccat taccgccacg ccgctgacca
    14821 tcaacgtcac cgccaccacc agcaaattca aaagcagcaa tcgcccgcgc gcgctggcaa
    14881 aaaagtgacg tccggggtgc gccatatcac gctccgtgaa gaaaaatcgc aatatgacaa
    14941 atatgaacgg cttctgacat tttgcattta ctttcctgtg atggagtgct gatatcgaca
    15001 acctcgcagg agtccaccat gaacagcacg ccagttctgg agatgcgcaa tattgccaaa
    15061 gccttcggca aattttatgc actcaaaggg gtggatttga cggtctaccc tggcgagatc
    15121 catgccctga tgggggaaaa cggcgcggga aaaagcacgc tgatgaaggt gctggcgggg
    15181 gcgtataccg ccaccagcgg cgagattctc atagacggca agccctttca cattcgcacg
    15241 ccaaaagatg ccttaagcgc cggtattacg ctgatttatc aggagatgca gctggcaccg
    15301 aatctgtcgg tggcagaaaa tatttttctc ggcagcgagc tttcccacgg cgggctggtg
    15361 cagcgtaaag agatgctagt gcaggcgcaa aaagtgatcg accgcctcgg cgcgcagttt
    15421 aacgccagcg ataaggtcat gacgctgacc attgccgagc aacagcaggt cgaaatcgcc
    15481 cgcgcactac atcgcaacag ccgcattctg gtgatggacg aacccaccgc tgccctctcc
    15541 tcccgcgaaa ctcaccgcct gtttgaactg attatgcggc tgcgcgatga agggatggcg
    15601 attatctaca ttagccaccg catggcggaa gtgtatgagc tttccgatcg cgtcagcgtg
    15661 ctacgcgacg ggcaatacgt tggcagcctg acccgcgata acctcaatgc cggggagctg
    15721 gtgcggatga tggtcggcag gccactgagc gatctgttca ataaagagcg cgatatcccg
    15781 ctcggtaaag cccgcctgaa tgttcaccac ctgaccgacg gcggcaaagt ccagccgagt
    15841 agcctgctgg tgcgttccgg cgaaattgtt ggcctcgccg gactggtggg tgccggacgt
    15901 tccgaactgg cgcagttgat cttcggcgtg cggaaagcga caggcggaat gattgaagtc
    15961 gatggtgaac cggtggtgat ccactccccg cgcgaagcca tcgatcttgg cattggtttt
    16021 ctcaccgaaa accgcaaaga acaaggctta ttccttgaaa tggcagcagc cgaaaacatc
    16081 accatggcaa ccctggagcg cgatgcccgc tggggaatgc tcaatcgcaa aaaagcgcaa
    16141 accatttccg atgacgccat taagttgctc aacattcgcg tgcctcatgc ccaggtacgc
    16201 gcgggcgggc tttccggcgg caatcagcaa aaactgttga tctcccgctg ggtggcgatt
    16261 ggcccgcgca ttttactgct cgatgaaccc gcccgcggcg tggacgttgg cgccaaaagt
    16321 gagatctacc ggatcatgaa cgagatggcg cgcaagggcg tggcgatcct gatgatctcc
    16381 agcgaactgc cggagatagt gggaatgagc gatcgcgtct atgtgatgcg cgaaggcagc
    16441 attgccggtg agttaaacgg caaaaacatc acccaggaaa acattatgac cttagcgact
    16501 ggcgtcaacg acgcccattc ccaggcggta acctcatgac gacctctgca aaaacctctg
    16561 ccaaaaaaat gctgatgagc gatctgatgc aaacggtcgg catcttgccc attttgattc
    16621 tgatcgtggc ggtatttggc tttatcacgc caaacttttt taccgaaagc aacctgctga
    16681 atattacccg ccaggcatcg atcaacattg ttctggcggc ggggatgacc ttcatcattt
    16741 tgaccggcgg gatcgatctt tccgtgggtt cgatcctcgg caccacagcg gtggcggcga
    16801 tggtcgtctc ccttagccct gagttcgcca tgctgtcgat cccggcagcg ctgatgctcg
    16861 gcctgctgct ggggctgttc aacggcgcgc tggtggcctt tgccggattg ccaccgttta
    16921 tcgttacgct tggcacctat acggcgctgc gcggggcggc gtatctgctg gcggacggca
    16981 ccacggttat caactccaac attaatttcg agtggatagg caacaactat ctcgggccgg
    17041 tcccatggct ggtggtgatt gcgctggcgg tgatcgtggt gtgctggttt attctgcgcc
    17101 gcaccacgct tggcgttcat atctacgccg tcggcagcaa tatgcaggcg gcgcgtctta
    17161 ccggcattaa ggtctggctg gtgctgctgt ttgtttatgg catgagcggc ctgctttctg
    17221 ggcttggtgg gattatgagc gcctcacggc tgtacagcgc caacggcaac ttaggcatgg
    17281 gttacgaact ggatgccatt gcagcggtga tcctcggcgg caccagtttt gtcggcggca
    17341 tcggcaccat caccggcacg ctggtcggcg cgctaattat cgccacgctc aacaatggca
    17401 tgaccttaat gggcgtctct tacttctggc aactggtgat caaaggggcg atgatcatca
    17461 ttgcggtgct gatcgacaaa taccgtaccc gacaccatca aagtgcataa acacatgagg
    17521 aatacagcat gagattgaag cccttagtga ccgcgctctg tgcaggcgcg ctgctggccg
    17581 cagcaccgtt cgcccaggcg aaagaactga aatcaatagg cgtaacggtg ggcgatctgg
    17641 cgaacccgtt cttcgtgcag atcaccaaag gcgcggagct ggaagcacgc aaactggcag
    17701 gcgataacgt caaagtgacg ctggtttcca gcggctacga tctgggtcag caggtgtcgc
    17761 agatagacaa ctttatcgcc gctaacgtcg atatgattat cctcaacgct gccgattcaa
    17821 aagggatcgg cccggcggtg aaacgggcga aagatgccgg gatcgtggtc gtcgcggttg
    17881 acgtagcggc agaaggtgcc gatgccacca tcacttccga taacacccag gcaggcgaaa
    17941 tggcctgtaa atacatcacc gatcgcctga aaggcaaagg caacgtggtg atcatcaacg
    18001 gcccgccggt atccgccgtg caaaaccgcg tcgaaggatg ccagactgaa tttaaacgcc
    18061 atcctgacat caaagtgctc tctgacaacc agaacgcgaa aggcagtcgt gaaggcgggc
    18121 tggaggtaat gacgggactg ctggcggcaa atccgaagat cgacggcgta tttgcgatta
    18181 acgatcccac cgcaatcggt gccgatctgg cagcaaaaca ggcacaacgt aacgagttct
    18241 ttattgtcgg tgtggacgga agcccggacg ccgaagaggc gctcaagcgt gagaactcgt
    18301 tgtttgtcgc tactccggcg caagatcccc aggtgatggc agcgaaagcg gtggaaattg
    18361 gctacgacat tttgcagggc aaacctgcgc cgaaagagcc ggtgctgatc ccggtgacga
    18421 tgatcgataa aaacaacgtc ggcaattaca aaggctggac ggtgaaataa gtccacacgc
    18481 cgggcggcgc agctgcccgg tcaccgctaa ggagaacgct ttcgatgaaa cgctccgcta
    18541 taaacgacat tctcggccat acacggcaat tcttttcgca acatgacgta catctgccgc
    18601 cctttgccag tttctcgcct gctcaatggc agcagctcga caccgccgcg tgggaggagg
    18661 tttttgatct taaactcggc tgggatgtta ccgcttttgg tcgcaacaac tttgcagcac
    18721 acgggctgac gctgtttacc ctacgtaacg gctcagcaaa aggaatgccg tatgtgaaat
    18781 gttacgccga gaagatcatg catgtgcgcg atgcgcaggt cacgccaatg cattttcact
    18841 ggcgtaagcg tgaggacatt atcaatcgcg gcggcggtaa tttaattgtt gaactgtgga
    18901 atgccgatag taacgagcaa acggctgata gtgacattac ggtggtgata gacggctgcc
    18961 gccagaaaca tactgcaggc tctcagttgc gcctgtctcc cggcgaaagt atctgcctgc
    19021 cgcccggcct gtatcacagc ttttgggcag aagccggttt tggcgatgtg ctggttggcg
    19081 aagtctcttc tgttaatgac gacgaccacg ataaccactt tttacagcca ctggaccgct
    19141 acaacctgat tgacgaagac gaacctgcac agttggtatt gtgtaacgaa taccgccagt
    19201 tccgctgaat aaggataacg ttatgccatt gatttctctc gctgaagggc tggcacatgc
    19261 ccgcgaacat cactattcgt taggtgcgtt taatgtgctg gactcgcact ttttacgcgc
    19321 cttgtttgct gctgccagac aagaacgttc gccctttatt atcaacattg ccgaagtaca
    19381 ttttaaatat atctcgctgg attccctggt cgagacagtg aaatttgaag cggcgcgtca
    19441 tgacattccg gtggtgttaa atctcgatca cggactgcat tttgaatcgg tggtgcaagc
    19501 gttgcggctg ggattcagct cggtaatgtt tgatggctcc acgctggggt atgaagaaaa
    19561 tgtccgccag acgcgggaag tggtgaaaat gtgccatgcc gttggcgtat cggtagaagc
    19621 tgaactgggc gcggtgggtg gcgacgaagg cggtgcactc tatggtcatg ccgatgaatc
    19681 cctgtttacc gatccgcaac tggcgcgtga ttttgtcgac cgtaccggca ttgacgcgct
    19741 ggccgtggcg attggcaatg cacacggcaa atatcgcggc gaaccgaaac tcgatttccc
    19801 gcgcctcgac gctatccgcc agcaggctgc tatcccgctg gtattacacg gcggctcagg
    19861 gattagcgat gccgatttcc gccgcgccat cgaactgggt atccacaaaa tcaatttcta
    19921 cactggcatg tcgcaagcgg cgctggctgc cgttgaggcc cgaatggcgc accgtcagcc
    19981 gatgtatgac gagtttgccg agttactgct ggcagtggaa gccgccatca gcgatacggt
    20041 tgcacaacag atgcgtattt ttggcagtgc ggggcaggca tgatggagcg taaagggatt
    20101 atcgccgcag gcaatatgtt ggttgaccac gtccatcaga ttatccagtg gccagagcgc
    20161 ggctggctgg cggagatcac ccacagcgaa cgctccaccg gcggtgcgcc gcttaacgtc
    20221 ctgctgacgc tggcgaaaat gcgcaccggg ctgccgttac aggcggttgg gctaatcggt
    20281 caggacagcg acggcgatta catcatggcg atgcttgagc aatatcacgt caatcgtcag
    20341 catgtgcagc gcactacctt tgccccgacg tcgatgtcgc aggtgatgac agacccaagc
    20401 gggcagcgga cgtttttcca ttcgccgggt gcaaacagac ttttggatct tccggcgttc
    20461 gatcagctcg ataacaccat gaagattttc catctcggct atttactgtt gctcgacagc
    20521 ctggatatgc cggacgatgt ttacggcacc cgtagcgcac gcctgctggc gcaaatgcgt
    20581 gatactggct ttgaaacctc actcgacctg gtctcccgta agggcgatcc gcgttatcag
    20641 ccgctggtgc tgcccgcgtt gcgccatctg gattatctgg taattaacga actggaggcc
    20701 ggggagttta gcgggctgga aattcggtta cccaacggcg aaccgcatat cacgcatatt
    20761 gctgccgccg cgcgtgaact gttagatgcg ggcgtgcgcc agcgggtggt gatccactac
    20821 ccggaagggg cgtggggaga gaccccagaa cagggcggcg tgtggatccc ttcgcaaaag
    20881 ttagaacaac gggaaattat cggcagcgtc ggcgcgggcg atgcattttg tgccggattt
    20941 ttatatggct gccatgaacg ctggacgctt accgagagca tcaaactggc acacgcctgc
    21001 gcgcgggcaa gcctgttatg cgccaatgcc atcgacgggg cgaaaacgct ggaagagctg
    21061 caaacagaaa tgtcggatta atgcacgtcg gcagcgaaga cataccccag accgcgcaga
    21121 gttttaatca acatcggctg atgcgggttg agttcgattt tgcgtcgtaa gcgcatgatt
    21181 aatacgtcga tggtgcgatc aaacacctcc acgctgtcgc tgtgggtgaa cgccagcaac
    21241 tgctcgcggc tcaatacccg acgcgcgttt tgcgccagag ccagtaacaa gccatattcg
    21301 ccctgagtta actcgacatg ttgctgaagc ggattgtgca actggcagcg catggtatcg
    21361 agataccagc cattgaatga cagcccggcg cttttctcgt cattaaccgc cagcgcgcca
    21421 acccgtcgca acaccgcttt cagtcgtgcg accaccaccc gcggggtgaa cggtttgcag
    21481 atgtaatcat ccgcgcccat ttccagcccg accaccacat cagactcact gcccatgccg
    21541 gtcaacatca ccaccggcaa tgtcggacgc agtttctgga tttgctgcaa taccctcagg
    21601 ccgttggtat cgggtagcat catatccagc accaccagcg caatgtcagg atcgccctca
    21661 atacgcaata tggcctcctg tccggtatgg cagactgaaa ccgtaaagac atgttcactt
    21721 aacacgtcct gcaacagtgc gcagatagcc gtgtcatcat ccaccaccag cacgacgggt
    21781 ttcatggtta cactccgcat cattcatctg attaatcatt gtgcgtactt cttgtcgaag
    21841 tgaaagctga aagtaatata aataataaaa atataagtca ggtcacatta ttgatgatag
    21901 taataaatat gtcgtacttt tccctcttca gcacaaatgc ctaataaaat ggatcgttcc
    21961 gctcacactt ccacttatta aagtcgcaat ttcccaatta tgataacgcc agctttaaat
    22021 gacgtatttt gcatggatta catatttacg acataaatga taaatggaga tttatatgaa
    22081 taactctcgg ttattccgtt tgagcaggat tgttattgcg ttaactgccg ccagcggcat
    22141 gatggtaaat accgctaacg cgaaagagga agcgaaagcc gccactcaat atacccaaca
    22201 ggttaatcag aattacgcca aatcattacc gtttagcgat cgtcaggatt ttgacgatgc
    22261 ccagcgtgga tttatcgccc cgctgctgga tgaaggtatt ctgcgtgatg cgaacggtaa
    22321 agtttactac cgcgcagacg attacaaatt tgatattaat gccgctgcgc cggaaaccgt
    22381 aaaccccagc ctgtggcgtc agtcgcagat caacggcatt tctggcctgt tcaaagtcac
    22441 cgataaaatg tatcaggtgc gcggccagga tatctctaac attacgttcg ttgaggggga
    22501 gaaaggcatt attgtcatcg acccgctggt gacgccgcct gccgcaaaag ccgcacttga
    22561 cctttacttc cagcatcgtc cgcaaaaacc gattgttgcc gttatctaca ctcacagcca
    22621 caccgaccac tatggtggcg tgaaaggcat tatctctgaa gccgatgtta aatccggcaa
    22681 agttcaggtg attgcccctg ctggctttat ggacgaagcc atcagcgaaa acgtgctggc
    22741 gggtaacatc atgagccgcc gtgcgctcta ctcttacggt ctgttactgc cgcacaacgc
    22801 gcaaggcaac gtgggtaatg gccttggcgt gacgctggca acgggcgacc cgagcattat
    22861 tgcaccgact aaaactatcg tcagaactgg cgagaagatg attatcgacg gcctggagtt
    22921 tgacttcctg atgaccccag gtagcgaagc gccagccgaa atgcacttct atattccggc
    22981 cctgaaagcc ctgtgtaccg ccgagaacgc cacgcatacc ctgcacaact tctacactct
    23041 gcgcggcgcg aaaacccgcg ataccagcaa gtggaccgag tacctgaacg aaacgctgga
    23101 tatgtggggt aacgacgcgg aagtactgtt tatgccgcac acatggccgg tctggggcaa
    23161 taagcatatc aatgattata ttggtaaata tcgcgatact atcacgttca ttcacgacca
    23221 gaccctgcac ctggcgaacc agggctacac catgaatgaa atcggcgaca tgattaagct
    23281 gccgcctgca cttgccaata actgggccag ccgtggctat tacggttctg tcagccacaa
    23341 cgcccgcgcg gtgtataact tctatcttgg ctattacgac ggtaacccgg ctaacctgca
    23401 tccgtatggt caggtagaga tgggcaaacg ttacgtgaaa gcgctgggcg gctctgcccg
    23461 tgtcatcaac ctggcgcaag aagcgaataa gcaaggtgat taccgctggt cggcagaact
    23521 actgaaacag gtgattgccg ccaacccggg tgaccaggtg gcgaagaatc tgcaagcgaa
    23581 taactttgaa cagctgggct atcaggccga gtccgccaca tggcgcggtt tctacctgac
    23641 cggcgcgaaa gagctgcgcg aaggggtgca taagttcagc cacggcacca ccggttcccc
    23701 ggacaccatt cgcggaatgt cggtcgaaat gctgttcgac tttatggccg ttcgcctcga
    23761 tagcgcgaaa gctgcgggta aaaatatcag cctgaacttc aatatgagca acggcgataa
    23821 cctcaacctg acgctgaacg atagcgtgct taactaccgt aaaacactgc aaccgcaagc
    23881 cgacgcctct ttctacatca gccgtgaaga tctgcacgcc gtgctgaccg gacaggcaaa
    23941 aatggcggat ctggtgaaag cgaagaaagc caaaattatt ggcaatggcg cgaaactgga
    24001 agaaattatc gcctgtctgg ataatttcga tttgtgggtg aatatcgtaa ccccaaatta
    24061 atgtcactcg cttttggggg cgctgaagcc cccaaaagca tttattttaa aaatatatac
    24121 agcca
//
LOCUS       DBFIFM010000056        23861 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.056, whole genome shotgun sequence.
ACCESSION   DBFIFM010000056 DBFIFM010000000
VERSION     DBFIFM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.056"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..134)
                     /locus_tag="N7C74_001940"
     CDS             complement(<1..134)
                     /locus_tag="N7C74_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2431816.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     "
     gene            complement(312..1223)
                     /gene="gsiD"
                     /locus_tag="N7C74_001941"
     CDS             complement(312..1223)
                     /gene="gsiD"
                     /locus_tag="N7C74_001941"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HGU2431817.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1226..2146)
                     /gene="gsiC"
                     /locus_tag="N7C74_001942"
     CDS             complement(1226..2146)
                     /gene="gsiC"
                     /locus_tag="N7C74_001942"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HGU2431818.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIARRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2164..3702)
                     /gene="gsiB"
                     /locus_tag="N7C74_001943"
     CDS             complement(2164..3702)
                     /gene="gsiB"
                     /locus_tag="N7C74_001943"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HGU2431819.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3722..5593)
                     /gene="gsiA"
                     /locus_tag="N7C74_001944"
     CDS             complement(3722..5593)
                     /gene="gsiA"
                     /locus_tag="N7C74_001944"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HGU2431820.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAVARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5580..6545)
                     /gene="iaaA"
                     /locus_tag="N7C74_001945"
                     /pseudo
     CDS             complement(5580..6545)
                     /gene="iaaA"
                     /locus_tag="N7C74_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
     gene            6749..7984
                     /gene="moeA"
                     /locus_tag="N7C74_001946"
     CDS             6749..7984
                     /gene="moeA"
                     /locus_tag="N7C74_001946"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HGU2431821.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADITSGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLHRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            7984..8733
                     /gene="moeB"
                     /locus_tag="N7C74_001947"
     CDS             7984..8733
                     /gene="moeB"
                     /locus_tag="N7C74_001947"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HGU2431822.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHNLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFSENVLTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8912..9574)
                     /gene="fsa"
                     /locus_tag="N7C74_001948"
     CDS             complement(8912..9574)
                     /gene="fsa"
                     /locus_tag="N7C74_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGU2431823.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     ELHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTTEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPRA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            9705..10604
                     /gene="ybiY"
                     /locus_tag="N7C74_001949"
     CDS             9705..10604
                     /gene="ybiY"
                     /locus_tag="N7C74_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HGU2431824.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQSLTVCGE
                     VKSVEEIMVTVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10610..13041
                     /gene="ybiW"
                     /locus_tag="N7C74_001950"
                     /pseudo
     CDS             10610..13041
                     /gene="ybiW"
                     /locus_tag="N7C74_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
     gene            13187..14002
                     /gene="ybiV"
                     /locus_tag="N7C74_001951"
     CDS             13187..14002
                     /gene="ybiV"
                     /locus_tag="N7C74_001951"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HGU2431825.1"
                     /translation="MSVKVIVTDMDGTFLNDVKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14154..15419
                     /gene="ybiU"
                     /locus_tag="N7C74_001952"
     CDS             14154..15419
                     /gene="ybiU"
                     /locus_tag="N7C74_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HGU2431826.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPSQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(15587..17179)
                     /gene="ybiT"
                     /locus_tag="N7C74_001953"
     CDS             complement(15587..17179)
                     /gene="ybiT"
                     /locus_tag="N7C74_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HGU2431827.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            17398..18318
                     /gene="ldtB"
                     /locus_tag="N7C74_001954"
     CDS             17398..18318
                     /gene="ldtB"
                     /locus_tag="N7C74_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU2431828.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18376..19494)
                     /gene="ybiR"
                     /locus_tag="N7C74_001955"
     CDS             complement(18376..19494)
                     /gene="ybiR"
                     /locus_tag="N7C74_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HGU2431829.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVTL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(19491..19958)
                     /gene="mntR"
                     /locus_tag="N7C74_001956"
     CDS             complement(19491..19958)
                     /gene="mntR"
                     /locus_tag="N7C74_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HGU2431830.1"
                     /translation="MSRRAGTPTAKKVTQLVNEEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20144..20272
                     /gene="mntS"
                     /locus_tag="N7C74_001957"
     CDS             20144..20272
                     /gene="mntS"
                     /locus_tag="N7C74_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HGU2431831.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            20544..22127
                     /gene="opgE"
                     /locus_tag="N7C74_001958"
     CDS             20544..22127
                     /gene="opgE"
                     /locus_tag="N7C74_001958"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HGU2431832.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTKGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22176..22691)
                     /gene="ompX"
                     /locus_tag="N7C74_001959"
     CDS             complement(22176..22691)
                     /gene="ompX"
                     /locus_tag="N7C74_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HGU2431833.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(22744..22809)
                     /locus_tag="N7C74_001960"
     CDS             complement(22744..22809)
                     /locus_tag="N7C74_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2431834.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23044..>23861
                     /gene="rhtA"
                     /locus_tag="N7C74_001961"
     CDS             23044..>23861
                     /gene="rhtA"
                     /locus_tag="N7C74_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HGU2431835.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAI"
BASE COUNT         5809 a         6538 c         5941 g         5573 t
ORIGIN      
        1 ctatcggcac gttttaccgc gcgttccgat accagttgat agttaatgat gaaaaaaagg
       61 ccaataaatg acagtgctgc caataaaaat aaaattatca gccttatctt tatcttttcg
      121 tataaactca gcatgttaat acatttaaca aaataactgt ctgaacgctg tgccgctgca
      181 aaaacagtcc cttgctgcgg ctatttaacc aaattacccg attctacaaa atcagcagaa
      241 agttacaaca cttgcttagg gcaataattt cctgtgaggt gattactctt tcaagcaata
      301 ttcaaacgta actatccttt aattttcgga tccagcgcat cgcgtaaacc atcgcccaac
      361 aaattgaacg ccagtacagt cagaaaaata gccagggccg gaaaaacagc gacatgcggc
      421 gcgataacca tatccgctcg agcctcattg agcattgctc cccactctgg tgtcggcggc
      481 tgcgcaccga ggccgagaaa tgacaggctg gcggcagaga taatcgaggt accaatgcgc
      541 atggtgaaaa acaccacgat agaagagacg gtcccaggca ggatatgacg caacaaaatg
      601 gtcatatcgc tggcaccaat actgcgtgct gactcaataa aggtttgctg tttcaacacc
      661 agcgtgttgc cgcgcaccag gcgggcaaac gcagggatgg aaaaaatggc gactgcaata
      721 atcacgttag caatgccgct tcccaacacc gcaacaacag cgatcgccag taaaataccc
      781 gggaaggcaa acagcacatc gcaaatgcgc atgatcagcc gatcccacca gccttcataa
      841 tatccagcca acaagcccag caacgtccct atcgccgcac cgataaacac ggcaaacacg
      901 cccgccgcca gcgagatttg cgcaccaacc aggacacggc tgaaaatgtc gcgccccagt
      961 gaatcgacgc caaaccagtg ctgcaaagaa ggtccgttat tcagattgtc ataatcaaaa
     1021 taattttcgg cgtcataggg agcgatccag cgtgcaaaaa tggccaccac aatcaataaa
     1081 ataacgaata atgcggcggt catcgccata tgctgacggc gaaatcgtcg ccagaattca
     1141 tgccacggtg tacgtacctg gtcaggtttg accagtggca tggcgtttaa caccgcctga
     1201 cgtcgccagt taaatagtcg catccttact tgtacctgat agccgggtta atggcggcgt
     1261 aaagcacatc caccactaag ttgataagaa taaattccag cgagaaaagc agaatttccg
     1321 cctgaatcac cgggtaatcg cgcatttcta cggagtcaac gagtaagcgt ccaagtccgg
     1381 gccagttgaa aactttctcc accacgatgg aaccaccgag caaaaagcca aactgtaagc
     1441 ccatcatggt cactaccggg atcatcgcgt tgcgtagccc gtgtttgagg acaacccagg
     1501 tttcgctcac ccctttcgcc ctcgcggtac gcatataatc ttcgcttaaa acgtcgacaa
     1561 acgacgcgcg ggtaaagcgc gccatcacgg cggccactgc cgcgccgagc gtcagggagg
     1621 gtaaaatgta gtgctgccaa ctgtctgctc ccacggtagg cagccagccc agttcaacgg
     1681 agaatacctg aattaaaagc atccccagcg caaatgccgg aaacgagatc cccgacaccg
     1741 caatggtcat actcaatcga tccggccaac ggttacgcca gacggcggcg ataattcccg
     1801 ccgccatacc aaatatcacc gcccagacca tactggttat ggtcagccac agcgttggca
     1861 taaagcgcct ggcaatctca tcggcaaccg gacgacgtga caccatcgac aggccaaaat
     1921 ccccctgcac agcattgctg atatagtgcc agaactggtg atacagcggc tgatccaacc
     1981 ccagctgctg acgcaccagt tctataacct gcgcatcagc ttcgggcccg gcaatcaatc
     2041 gcgccggatc gccgggcagc atatgcacaa ataaaaacac cagcaccgag acgataaaca
     2101 gcgtcggaat caaccccagt aatcgtttga taacgtaatt aagcattcca ctccctgcgt
     2161 tgcttattgc aaatccgcgt cttcaaagct gaagccggta tctggcatga tccaaaaacc
     2221 ggtcaggttt ttactgtgtg ccgacaccag tttttctacc accagcggga tccacggcga
     2281 ctcttgccag atgatatcct gcgccgcctt atataagcgg gtcttttccg ccggatcatt
     2341 agttttcagt gcctgagcca ggaagtcatc cacctgttta ttgctgtaaa acgcggtatt
     2401 aaacagcgtc ggtggccagt tttgcgaggc aaacaacggc gatagtgccc agtccgcttc
     2461 gccggttgaa gccgaccagc cagtgtagaa catccgcacg ccgctctctt tttgcccttt
     2521 accttcaact tctgccgccc gctgtccggc atccatcgca gtcacctggg ctttaatccc
     2581 gacctgcgct aactgctgct gggtaaattg cagcactttc tgcgcggtgc tgtggttatg
     2641 tgacgaccac agcgtggtac tgaaaccgtt gggatatccc gcctctttca gtaattcgcg
     2701 cgctttcact ggatcgtaag gccacggttt ataactttgc gcgtaggcga tactcggcgg
     2761 taccacacca gtagctggcg ttgcatagcc cgcgaaggcc actttcacca gcgccggacg
     2821 gttaatggcg taattcagcg cctcacggac cttcgggtta tcgaatggct tttgcgtcac
     2881 gttcatactg atataacgct gcataattga cggactggcc atcaactcga tatttttgtt
     2941 tttctccagc agtgcggcct gctcgtaagg aatggggaaa gcaaactgcg cttcaccggt
     3001 ttgcagcatt gccgcgcggg tgttgttatc cgccaccgga cgccaggtta tgctgtccag
     3061 tttgggcaat cctggctgcc agtaacccgc gaattttttc accttcacaa aatcggtctg
     3121 attccaggta tccagttcat acggtccggt tcccaccgga tgaaaaccaa tctccttgcc
     3181 atatttttcc agcgctgccg gtgaaatcat cgcggtcgcc ggatgggcaa gaatattaat
     3241 aaacgctgag aacggctgtt tgagggtaat ctttaccgtt gtcggatcga tcgcttccgt
     3301 tttagcaata ttcttataca ggttatagcg tttaagatga ttcgccggat cgctggcccg
     3361 gtccagattc gcttttaccg ccgctgcgtt gaaatcggtg ccatcctgga atttaattcc
     3421 ttcccgcaat ttcacggtgt aagtaatgcc gtcatcggaa acggtataac tctccgccag
     3481 cacgtttttc agtttcatct ctttatccag accgaacagc ccctggtaaa acgatttcgc
     3541 tacggcctga gataacgtgt cattcgcgtc atacggatcg agcgtggtga aattcgatcc
     3601 taccgccacc accacatctt tggcagcgaa tgcacaagat gccatcaacg ctgtcgcaat
     3661 gcccagcgcc actaacccac tacggtgtac agctcttgcc attttattct ccgcctgaat
     3721 gttatctacg catgaatgcg tattctgatt gtggttgtgc gacgtaatgc cccggcccga
     3781 cgcattgcaa cgagacggct gccacctctt cgccacgcag atgaatattg ctgggaagat
     3841 cgtccgacag cagtacacgc tgcggtcgtt gtcgggacgg ttcagcgacc ggaactgccg
     3901 ccagtaattt acgcgtataa ggatgctgcg ggttttcgaa gaccgcgcgc cgtggaccaa
     3961 tttcaacaat ttgcccgaga tacatcaccg ccacacgatg actaatccgc tctaccacag
     4021 ccatatcgtg ggagataaac agatacgcaa tgccgaaatc acgctggaga tcgagcaaca
     4081 agttgataat ctgcccgcga attgaaacat ccagcgccga aacggcttcg tcggcaatga
     4141 tcacttttgg attcaatgcc aacgcgcgag caatgcagat gcgctggcgc tgaccgccgg
     4201 aaaactcatg cgggtaacgc caggcatgtt caggtaacag gcccacgcgc tccagcaacc
     4261 acgcaacgcg tgcaaccgct tctttacctg gcaataaacc gtgtacgcgc agcggttcga
     4321 taatcgaatc accgatggtc tgacgtgggt ccagcgaagc gtaagggtcc tgaaaaataa
     4381 actgaatatc gcggcgcaat gcctgaagtt tgccgggtga caaggtatcg attcgctgac
     4441 cgttaaagat aatttcgccg ccctgcgatt cgaccaggcg cagcaacgcc cgcccggtag
     4501 tggatttacc gctgccagac tcgcccacca gcgatagcgt ttcgccaggc cagagatcaa
     4561 aactgacttt ctcaacggca tgcacttccc gcgttacgcg attcaacaaa ccgctgcgca
     4621 aagggaaacg ggtgaccaga ttacgcaccc gtaaaacagg ttcgccatcc accaccgttt
     4681 tctgctcgat ggggggggcc tgtttcgctg gatgttcaag cgatatcaat gggaaacgtc
     4741 ggggataatc taaccctttc atcgcaccaa gttgcggaac agcagctaac agcgcacggg
     4801 tgtagggatg ttgcggtgca tgaaaaatct gttcgacggt acccgtttcc accgcctcgc
     4861 cctgatacat caccagtacc cgatcggcaa tctctgccac cacgcccata tcgtgagtga
     4921 taaagataac gcccatcgac atctcttttt gcaatacttt gattaattgc aggatctgcg
     4981 cctgaatagt gacatccagc gcggtggttg gctcatcggc tatcagcact gccgggcggc
     5041 atgacagcgc catcgcaatc atcactcgct ggcgcatccc gccagagagt tgatgcggat
     5101 aacgtgaaag aatggtttgt gcctccggaa tgcgtacctg atccagcatc cgcttcgcct
     5161 cgaccatcgc ttcttcacga ctggcgttct gatgcagacg aattgattcg gcaatctgtt
     5221 cacccacagt aaatactggg ttcagcgatg tcatcggctc ctgaaatatc atcgccatat
     5281 ccgcaccgcg cacatggcgc atttgtgcag cgctctgctc gctaagttca atcacatcac
     5341 gactgcgccg ccgcaacagc attttatcgc actgcactaa accgcccgcc tgttccaaca
     5401 gacgcatcaa tgccagcgca gtcactgact taccggagcc ggattcgcca acaattgcca
     5461 gcgtctcacc gcgttgcaga ctaaaagaga gattgcggac cgcagctatt ttctgctggt
     5521 cctgcataaa ggcaatatta agattttcaa ccgccagcac attaccggca tcaagttcat
     5581 cactgtgtgg caacggtgtc ccctttttca cggtagatac cggtggttgg cgtatcgcct
     5641 gcgtagcccc aggcgcgata cattccttcg gtgttaaacg gtagcgcgac attcccttca
     5701 tggtcgatag cgattaagcc accgctaccg ccaagcgcag ggagtttttc cattactacc
     5761 cgctcgcagg cttccgcgag acttaatccg ccgtaatcca ttaacgcggc gatgtcatat
     5821 gccgccagcg cgcggatgaa gacttcgccc gtgccggtac aagaaaccgc cacactggcg
     5881 ttattggcgt agcatccggc acccactaag gggctatcgc caactcgtcc gggtaattta
     5941 ttggtcattc cgcccgtgga cgtggctgcc gccagattgc cgtctaaatc caacgccacg
     6001 gcccccacgg tgcccatttt ctgtttttca tccagtggcg caccgctatg gtcgaggact
     6061 gttgcccctt cctcgcgcgc tgccattagt tgttcataac gcaaaggcgt ggagaaaatc
     6121 tccggtgaga cgcgctccat gccatgagca aacgcaaaat tttctgcccc ttcgccaatc
     6181 atcatcacat gcgggcttta ctccatcacc agccgggcgg caagaaccgg attacgcaga
     6241 tgactaacgc ccgccaccgc accggctttc agggtgttac catccatcac acaggcgtcc
     6301 agttcatgcg tttcatcacg cgtaaagacg gcgccaattc cggcgttaaa cagtggacac
     6361 tcttccagca gacgcaccgc ttccgtcacc acatccagcg cactttcgcc cgcttccagc
     6421 attttctgcc cggtttcaac aatggcagac aacgcctcga tgtagcgtaa ttcctgttgc
     6481 agactcatct gcgcgcggct aattgcacct gcgccaccat gaattgcaat gactgctttg
     6541 cccatagtat tcgtccatta agggtggctc gccacttttg ctataaatat cagaattttt
     6601 atgaattact cattcaattt ttgaatatag aaagatggat atggcatgta aaggcaggac
     6661 ccagcgctac gcccgaggtg gcatacagtc tgtggcgttt ttctgacata ataggcaaat
     6721 tcgattttgc ctccgcagga gtgttttcat ggaatttacc accggattga tgtcgctcga
     6781 caccgcgctt aatgagatgc tttctcgcgt caccccactg accgcccagg aaacgctgcc
     6841 actggttcag tgttttggtc gtattctggc gagcgatgtc gtttcgccgc tggatgtccc
     6901 agggtttgat aactccgcaa tggatggtta cgcggtacgt ttggccgata tcacctccgg
     6961 acaaccgctg cccgttgccg gtaaatcctt tgccggtcag ccgtaccatg gtgaatggcc
     7021 tgcgggtacc tgcattcgta ttatgaccgg tgcgccggtg ccggaaggct gcgaagcggt
     7081 ggtgatgcag gagcagactg aacaaacgga caatggcgtg cgttttactg ctgaagtgcg
     7141 tagcgggcaa aatattcgcc gtcgcggtga agatatctct gcaggtgcgg ttgttttccc
     7201 ggcggggact cgcctgacta ccgcagagct gccagtaatt gcttcactgg ggattgccga
     7261 agttccggtg attcgtaaag tgcgtgtagc gcttttttct accggtgatg aactccagtt
     7321 gccaggccag ccgctgggcg acggccaaat ctacgatacc aaccgtctcg ccgtacacct
     7381 gatgttagaa cagctgggtt gcgaggtaat caacttaggg attatccgcg acgatccaca
     7441 tgccctgcgc gccgcattta ttgaagccga cagccaggcg gatgtggtga tcagttccgg
     7501 cggtgtttca gtgggtgaag cggattacac caaaacgatt cttgaagagc tgggggagat
     7561 cgccttctgg aagctggcga ttaaaccagg taaaccgttc gcgttcggta aactcagcaa
     7621 tagctggttc tgcggcctgc cgggcaaccc ggtttcagcg acgctgacct tctatcaact
     7681 ggtacagcct ttgctggcaa aactaagcgg caacaccgcc agcggcctgc ccgcgcgcca
     7741 gcgcgtacgc acagcgtccc gcctgaaaaa aacgccagga cgtcttgatt tccagcgcgg
     7801 cgtgctgcac cgcaacgccg atggcgaact ggaagtgacg accaccggac atcagggttc
     7861 acatatattc agctccttta gcctcggcaa ctgctttatc gtgctggaac gcgatcgcgg
     7921 caatgtggaa gtgggcgaat gggtggaagt agaaccgttt aacgcgttgt tcggaggcct
     7981 gtaatggcgg aactcagcga tcaagagatg ctgcgctaca accggcaaat cattctgcgc
     8041 ggttttgatt ttgacggtca ggaggcgctg aaagattctc gtgtgctggt cgtcggcctc
     8101 ggtggactcg gctgcgcagc ttcgcagtat ctggcaagcg ccggtgtcgg caacctgacg
     8161 ctgctcgact tcgactcggt ttcgctctcg aatctgcaac gccagacgct gcacagtgat
     8221 gctacggtcg ggcaaccgaa ggtggaatcc gcccgtgacg ctctgacgcg gatcaaccca
     8281 catatcgcga ttacgccagt caatgcactg ctggatgacg cagaacttgc agcattgatt
     8341 gctgaacaca atcttgtgct cgactgtacg gataacgttg cggtacgtaa tcaactgaac
     8401 gcaggctgtt ttgccgcgaa ggtgccgctg gtttccggcg cggcaattcg tatggaaggg
     8461 caaatcacgg tcttcactta tcaggacggc gaaccgtgct atcgctgcct tagccgtttg
     8521 tttagcgaga acgtgttaac ctgcgtagaa gcaggcgtga tggcaccgtt gatcggcgtg
     8581 attggttcgt tgcaggcaat ggaagcgatc aaactgctgg caggttatgg caaaccggcc
     8641 agcgggaaaa tcgtcatgta cgatgcgatg acctgtcagt ttcgcgaaat gaaactgatg
     8701 cgcaatccgg ggtgtgaggt gtgcgggcag taattgccac ctccaggcct gataagacgc
     8761 gacaagcgtc gcatcaggca tttgctccag acgccagatg cggcgtgaac gccttatctg
     8821 gcctacggtt cgggcacaga tttccaagcg cggcaggcat cggtgcactc cgccacgtca
     8881 tcagcaattt atgaggatgc agaacgtccg gttaaatcga cgttctgcca aacgctccct
     8941 gccagtcctg ctcaaacttc gccacagcgg catcaaccgc cggatagcta atcatctgtt
     9001 gtgccacatc cagtggcaga gtaattgatt cacatcctgc cagtaagcag tccagcgcct
     9061 gacgcggggt tttgaaactc gcggctaaca ctttcgcccg cggcgcatgc attttcaata
     9121 actggtgtaa gtcggtcaca gtctgaatac cgctaccgcc ctgagcatca atacgattaa
     9181 cgtaaggcgc aacatattcc gcacctgcca gcgccgacag cagcccttgt gctgcgccat
     9241 ataccgcggt tcccagcgtc ggaatccctt ccgcttttaa catcttaata gctgccagcc
     9301 cctcggtggt caccggaact ttcaccacga tatccgcaat aatagaacgc aatttacgtg
     9361 cgtcattcac catcccttca gcagtggtag ccattacctg ggcaaacaga cgcccctgac
     9421 cgcccatcgc ttcatgaagt tccggaagca caacctccag cggtttttta cccgcggcga
     9481 taatgcttgg gttagtggtc acacccgcca gcggaaaaat acgtgacagc gccttcaccg
     9541 caacaacgtc tgaagtatcc agatacagtt ccataaccat cctcaaaatg tactttttgt
     9601 ctgaatgtag actatcacgg gaaaactctc tgaacagttg acgcacatca atctaacttt
     9661 cattcgaaag tattttaata tttgaacgaa acagagaggc aactatgatt ttcaatattc
     9721 agcgctactc gacccatgat ggccccggta tccgcacggt cgtatttctt aaaggctgtt
     9781 cgctgggctg ccgctggtgt cagaacccgg aaagccgcgc ccgcacgcag gatctgctgt
     9841 atgacccacg tctgtgcctg gaaggctgtg agctgtgcgc taagaccgcg ccggaagtga
     9901 ttgagcgcgc gctgaatggt ttgcttattc atcgggaaaa gttaaccccg gagcatctga
     9961 cggcgttaac cgactgctgc ccgacacagt cgttaaccgt gtgtggggaa gtgaaaagcg
    10021 ttgaggagat catggtgacc gttctgcgcg ataaaccgtt ttacgatcgc agcggcggcg
    10081 gcttaacact ttctggcggt gaaccattca tgcagccgga aatggcgacg gcgctactgc
    10141 aagccagcca cgaggccggc attcatactg cggtagaaac ctgcctgcat gtgccgtgga
    10201 aatatatcgc cccttctctg ccctatatcg atctgtttct tgccgattta aaacacgttg
    10261 ccgacgcgcc gtttaaacag tggaccgacg gtaacgccgc cagggtgctg gataacctga
    10321 aaaaactcgc cgcagcgggc aaaaaaatca ttatccgcgt gccgctgatt caaggcttta
    10381 atgccgacga aacctctgtg aaagccatta ccgattttgc cgccgacgag ctgcacgttg
    10441 gcgaaattca tttcctgccc taccacacgc tgggcatcaa caaatatcac ttacttaatc
    10501 tgccctatga cgccccggaa aaaccgcttg atgcgccaga actgctcgac tttgcccagc
    10561 agtatgcctg ccagaaaggg ttaaccgcga ccttacgagg ataacaacca tgaccacact
    10621 gaaactggac acgctcagcg accgcattaa agcgcacaaa aatgcgctgg tgcatattgt
    10681 gaaaccgccg gtttgtaccg agcgcgcgca gcactatacc gagatgtatc aacaacatct
    10741 cgataagccg atccctgtac gtcgcgcgct ggcgctggca catcacctgg cgaatcgcac
    10801 tatctggatc aagcacgatg agttgatcat cggcaaccag gcaagcgaag ttcgcgccgc
    10861 gccgatcttc ccggaatata ctgtctcgtg gatcgaaaaa gagattgatg atctggcaga
    10921 tcgtcccggt gctggctttg cggtgagcga agagaacaaa cgcgttctgc atgaagtgtg
    10981 cccgtggtgg cgcggtcaga cggtacagga tcgctgctac ggcatgttta ccgatgagca
    11041 aaaaggtctg ctggcgaccg gaatcattaa agcggaaggc aatatgacct ccggcgatgc
    11101 gcacctggcg gtgaatttcc cgctgctgct ggaaaaaggc ttgatggtct gcgcgagaaa
    11161 gtagcggaac gtcgctcgcg catcaatctg acggtactgg aagatctgca cggcgaacag
    11221 ttcctgaaag cgattgatat cgtgctggtg gcagtcagtg aacacattga acgtttcgct
    11281 gccctggcgc gtgcaatggc cgcgaccgaa acccgcgaaa gccgtcgcga tgaactgctg
    11341 gcgatggcag aaaactgcga tcttatcgcc caccagccgc cgcagacttt ctggcaggcg
    11401 ctgcaactgt gttacttcat ccagttgatt ttgcagatcg aatctaacgg tcactcagta
    11461 tcgtttggtc gtatggacca gtatctctac ccgtactatc gccgcgacgt tgaactcaac
    11521 cagacactgg atcgcgaaca cgccatcgaa ttgctgcata gctgctggct gaaactgctg
    11581 gaagtgaaca aaatccgctc aggctcacac tcaaaagcct ctgcgggaag tcctctgtat
    11641 cagaacgtca ccattggcgg acaaaatctg gttgatggtc aaccaatgga cgcggtgaat
    11701 ccactctctt acgcgatcct cgaatcctgc ggtcgcctgc gttccactca gcctaacctc
    11761 agcgtgcgtt accacgcggg aatgagcaac gatttcctcg acgcctgcgt acaggtgatc
    11821 cgttgcggct tcgggatgcc ggcgttcaac aacgacgaaa tcgtcatccc ggaatttatt
    11881 aaactcggta ttgaaccgca ggatgcttac aactatgcag cgattggttg tatcgaaacc
    11941 gccgtcggtg gcaaatgggg ctatcgctgt accggcatga gctttatcaa cttcgcccgc
    12001 gtgatgctgg cggcgctgga aggcgggcgt gatgccacca gcggcaaagt gttcctgcca
    12061 caagaaaaag cgttgtcggc aggtaacttc aacaacttcg atgaagtgat ggacgcgtgg
    12121 gatacgcaaa tccgttacta cacgcgtaaa tcaatcgaaa tcgaatatgt cgtcgacacc
    12181 atgctggaag agaacgtgca cgatattctc tgctcggcgc tggtggatga ctgtattgag
    12241 cgagcgaaaa gtatcaagca aggcggcgcg aaatatgact gggtttctgg cctgcaggtt
    12301 ggcattgcca acctcggcaa cagcctggcg gccgtgaaga aactggtatt tgaacagggc
    12361 gcgattagtc agcaacagct ggctgccgca ctggcgaatg acttcgacgg cctgactcac
    12421 gagcagctgc gtcagcggct gattaacggt gcgccgaagt acggcaacga cgatgatact
    12481 gtcgatacgc tgctggctcg cgcttatcag acctatatcg acgaactgaa acagtaccat
    12541 aacccgcgct acggtcgtgg tccggttggc ggcaactatt acgcgggtac gtcgtctatc
    12601 tccgctaacg taccgtttgg cgcgcaaact atggcaacgc cagacggacg taaagcccac
    12661 cccccgctgg cagaaggcgc aagcccggct tccggtactg atcatcttgg ccctactgcg
    12721 gtcattggct cggtgggtaa actgcctacg gcagcgattc tcggcggggt gttgctcaac
    12781 cagaaactga atccagcaac actggagaac gaatctgaca agcagaaact gatgatcctg
    12841 ctgcgcacct tctttgaagt gcataaaggc tggcatattc agtacaacat cgtttcccgc
    12901 gaaacgctgc tggaggcgaa aaaacatccc gatcagtatc gcgatctggt agtgcgtgtc
    12961 gcgggttatt ccgccttctt caccgcgctc tctccagacg ctcaggacga tatcatcgcc
    13021 cgtaccgaac atatgctgta atcccctcag cccggcggag acgtcgccgg gctaaatcat
    13081 tcacatcatc aatttcatcc ttactttcat tcgaaatata atttgtgctc tgcgtcacat
    13141 tgttattaca ctgttacggt ttcattttcg agccgggagc acacctatga gcgtaaaagt
    13201 tatcgtcaca gatatggacg gtacttttct taacgacgtc aaaacgtaca accaaccacg
    13261 ttttatggcg caatatcagg aactgaaaaa gcgcggcatt gagttcgttg tcgccagcgg
    13321 taatcagtat taccagctta tttcattctt tcctgagcta aaggatgaga tctcttttgt
    13381 cgcggaaaac ggcgcactgg tttacgaaca tggcaagcaa ctgttccacg gcgaactgac
    13441 ccgacatgaa tcgcggattg ttattggcga gttgctaaaa gataagcaac tcaattttgt
    13501 cgcctgcggt ctgcaaagtg catatgtcag cgaaaatgcc cccgaagcat ttgtcgcact
    13561 gatggcaaaa cactaccatc gcctgaaacc tgtaaaagat tatcaggaga ttgacgacgt
    13621 actgttcaag ttttcactca acctgccgga tgaacaaatc ccgttagtga tcgacaaact
    13681 gcacatagcg ctcgatggca ttatgaaacc cgtcaccagt ggttttggct ttatcgacct
    13741 gattattccc ggtctacata aagcaaacgg tatttcgcgg ttactgaaac gctgggatct
    13801 gtcaccgcaa aatgtggtag cgattggcga cagcggtaac gatgcggaga tgctgaaaat
    13861 ggcgcgttat tcctttgcga tgggcaatgc tgcggaaaac attaaacaaa tcgcccgtta
    13921 cgctaccgat gataataatc atgaaggcgc gctgaatgtg attcaggctg tactggataa
    13981 cacatcccct tttaacagct gacctctcta ccggagccat gcgctccggt tatcccttct
    14041 ttttacatcc tgtacatcac atcaaatcat taaacttgca ttaacttatt tataacttat
    14101 attgtcacat gcaaatcagc atagtttcga atgaaagtta atgcgaggtg gttatggctt
    14161 ctacttttac cagcgacaca ttgcctgccg atcacaaagc agctatccgt cagatgaagc
    14221 acgcgctgcg ggcgcagctt ggcgacgtcc agcagatctt taatcagcta agcgatgaca
    14281 ttgccacgcg agtggctgaa atcaacgcac tcaaagcaca gggcgatgcc gtctggccgg
    14341 tgctgtctta tgccgatatc aaagcaggtc atgttactgc agagcagcgc gaacagatta
    14401 aacgtcgcgg ttgtgcggtg ataaaaggcc atttcccccg cgaacaagcg ctaggctggg
    14461 atcagtcgat gctggactat ctggaccgca accgctttga cgaggtctac aaaggccccg
    14521 gcgataattt cttcgggacg ctcagcgctt cacgtcccga gatttacccc atctactggt
    14581 cgcaggcgca aatgcaggcc cgccagagtg aagaaatggc gaatgcgcag tcgtttctca
    14641 atcgtctgtg gacatttgaa agtgatggaa agcaatggtt taacccggat gtgagcgtca
    14701 tctaccctga ccgtatccgc cgccgtccgc ccggaacgac ctccaaaggt cttggagcgc
    14761 ataccgactc cggggcgctg gaacgctggc tgcttccagc gtatcagcac gttttcgcta
    14821 acgtctttaa tggcaatctg gcgaaatacg atccctggca tgcggcacat cgtacggaag
    14881 ttgaagagta cacggtggac aacaccacca aatgttccgt gtttcggaca ttccagggct
    14941 ggacagcgct ctctgatatg ctgcctagtc aggggttgct gcacgtcgtg cccattcctg
    15001 aagccatggc gtacgtactg ttacgtccgc tgcttgatga tgtgccggag gatgaactgt
    15061 gcggcgtagc gcccggaaga gtgttgccgg tatcagagca atggcatcca ctgttaattg
    15121 aggcgttaac cagcattcca aaactcgagg ccggagactc cgtctggtgg cactgcgacg
    15181 tcatccattc cgttgccccc gttgaaaatc aacagggttg gggcaacgtg atgtacattc
    15241 ctgcggcacc gatgtgcgag aaaaatcttg cctacgcgca caaggtgaag gccgcactgg
    15301 aaaaaggcgc atcgccgggc gacttcccgc gcgaggacta tgaaacaaac tgggaaggac
    15361 gctttacgct tgccgacctc aacattcacg gtaagcgagc gttgggcatg gatgtttgag
    15421 agttaattga agtgaattga tgtgggatta agaaaatcga ttcgacgtta ctcagattgc
    15481 tgacaagtgc gctttcttaa tgcctgataa gcatagcgca tcaggcaatc ttgcgtttgt
    15541 catcagccgc cgtcaccccg acagtatcgg ggtgacggat aataacttac tcgatccctt
    15601 tactacgcag gtaatcttcg taattaccgc taaagtcgat cacgcgttcc ggggtgattt
    15661 ccagaatgcg ggtcgccagg gagcttacga actcacggtc gtgtgaaaca aagatcagcg
    15721 tgccctgata cagttccagc gccatgttca gtgactcgat ggattccata tccaggtggt
    15781 tggtcggttc gtccatgatc agaatgttcg gcttctgcat cattaactta ccaaaaagca
    15841 tccgcccttt ttctccaccg gaaagcactt tagcaggctt tttgatgtcg tcctggctga
    15901 acagcaaacg accgagaata ctgcgcaccg cctgctcgtc atcgccttcc tgcttccact
    15961 ggctcatcca ttcgaacacg gtcagatcat tttcaaactc atattcgtgg tcctgagcat
    16021 agtaaccaat gcgcgcgttt tcagaccatt ttacggtgcc gctgtccggt tgcagatcgc
    16081 ccaccagcgt tttcaacagc gttgatttac cgacgccgtt ggtacccagt accgccagtt
    16141 tttcaccgac ttccagcagc aggttgagat ttttaaacag cggaccgtta tcaaaccctt
    16201 tggtcagacc ttccacttcc agcgcgttac ggaacagctt cttatcctgt tcaaaacgga
    16261 tgaacgggtt ctgacggctg gaggctttca cctcttccag tttgatttta tcaatctggc
    16321 gcgcgcgcga agttgcctgg cgagatttcg aggcgttggc gctaaagcgg ctaacgaaag
    16381 attgcagctc agcaatctgc gctttcttct tggcgttatc ggccagcaga cgttcacgcg
    16441 cctgggtcgc cgccgtcatg tactcatcgt agttacccgg ataaacgcgc agctcgccgt
    16501 aatccagatc cgccatgtgg gtacagacca tgttaaggaa gtgacggtcg tgcgagatga
    16561 tgatcatggt gctgtcacgc tcgttcagca cctgttccag ccagcgaatg gtgtcgatgt
    16621 cgaggttgtt ggtcggttcg tcgagcagga gaatatccgg atcagcaaac agcgcctgcg
    16681 ccagcaacac acgcagcttc cagccaggag caacttcact catcgggccg tagtgctgtt
    16741 ccactggaat tcccacgcca agcaacaatt caccggcacg agcttccgca gagtaaccgt
    16801 ccatttcgcc gtatttaact tccagatcgg ccactttata gccgtcttct tcactcattt
    16861 ccggcaaagc atagatgcgg tcgcgctcct gcttcacttc ccacaactct ttatgcccca
    16921 tgatcaccgt atccagcaca gtgaactctt caaaggcaaa ctgatcctga cgcagtttac
    16981 caatgcgctc gttgggatcg agggaaacgt tacccagcgt cggctcaagg tcgccgccga
    17041 ggatcttcat aaaggtggat ttaccactac cgttcgcgcc aatcaggccg taacggttgc
    17101 cgccgccaaa tttgacggaa atgttttcaa acaacggctt actgccgaac tgcatggtga
    17161 cgttactgga aactaacaca ggcgtatcct gaaaagagat atgacaaacc gcgcattatg
    17221 ccataagtct gaaagaagat cctgctcctc gcttaagatc tacactcaag ccaaaggcaa
    17281 aaaatgtgat ttcgtacaca tctgatttca ctgtgagctg gaatgaactt ataatgcgct
    17341 tccaatactc taatattctc aacccaatgg cctgccaggc acaaaatctc gcttaacatg
    17401 aatatgaaat tgaaaacatt attcgcagcg gccttcgctg ttgtcggctt ttgcagtacc
    17461 gcctctgcgg taacttatcc tctgccaacc gacgggagtc gcctggttgg tcagaatcag
    17521 gtgatcacca ttcctgaagg taacactcag ccgctggagt attttgccgc ggagtaccag
    17581 atggggcttt ccaatatgat ggaagcgaac ccgggtgtgg ataccttcct gccgaaaggc
    17641 ggtactgtac tgaacattcc gcagcagctg atcctgccgg ataccgttca tgaaggcatc
    17701 gtcattaaca gtgcagagat gcgtctgtat tactatccga aagggaccaa caccgttatc
    17761 gtgctgccga tcggcattgg tcagttaggc aaagatacgc ctatcaactg gaccaccaaa
    17821 gttgagcgta agaaagcagg cccgacctgg acgccgaccg ccaaaatgca cgcagagtac
    17881 cgcgctgcgg gcgaaccgct tccggctgtc gttccggcag gtccggataa cccgatgggg
    17941 ctgtatgcac tctacatcgg tcgcctgtac gctatccatg gcaccaacgc taacttcggt
    18001 atcggtctgc gtgtaagtca tggttgtgtg cgtctgcgta acgaagacat caaattcctg
    18061 ttcgagaaag taccggtcgg tacccgcgta cagtttattg atgagccggt aaaagcgacc
    18121 accgagccag acggcagccg ttacattgaa gtccataacc cgctgtctac caccgaagcc
    18181 caatttgaag gtcaggaaat tgtgccaatt accctgacga agagcgtgca gacagtgacc
    18241 ggccagccag atgttgacca ggttgttctt gatgaagcga ttaaaaaccg ctccgggatg
    18301 ccggttcgtc tgaattaatc ttcaaaacgt aaaacaaagg cggactcata atccgccttt
    18361 tttatttgcc agaccttacc aggccgggat cataaccaac aaaacatatc ccaccagcgc
    18421 cgcccacaac agcatcggta ttgaatagag atggaaacgc caccagatgc ggcgatcgtt
    18481 cgccatacgt agcgcaatca aatttgccag cgatccgggt aataacccaa agccacctac
    18541 gtttaccgcc catgccagta ataaagacgg cggcacatag ttcagcaaca atatagtact
    18601 cggcacgtta ctgatcacct gcgataaacc gattgccgtt aaccataatc cgggttcaga
    18661 taaatgactc acgttaccca acacgccttg caacgctgga agctgggtca gtaaatggac
    18721 gtcgataaac atcgccataa acaccagcag cagcgtccag tccacactca acaccacgcg
    18781 acgcgccagc agcgcaaagc ccgccgccac aatcaccagt ccccacagct cttgtttgaa
    18841 ctccagcgcc gtcagaaaga cgatatacag ccccagacaa ctccacacca gccgcggttt
    18901 ccactccggt gtttgcaccc ccgtatggta ttggagtgcc tttccaggga aacaacacca
    18961 gcacaacagc aggagcgtca gcatcattgc gccagccagc ggtgccattt gggcaataaa
    19021 tccggcaaac gaaagaccag aacgtcccca gataagaata ttttgcgggt tgccaattgg
    19081 cgtcagtagc gaaccagcgt tgactgccag cgcctcaaaa ataatcagcc gattaaccgg
    19141 gatctcacac agccttttta gcgtgatagt cagcggaaca acaataaaca gcgtgacatc
    19201 gttggtcaga aaggtagaaa gcagcgccgc cgccagcacc ataaacatcg ccagccgacg
    19261 ctccgtagca aagcggcgca ccattttgcg ccccagcaca tcaaaataac cgcttaactc
    19321 cacacctttg gtcagcagca tcaggccgct taaggtgatg atggtgtgcc agtcgatagc
    19381 agcaggccag gatttcggtg caaagggcac gaaaaaactt aatccgatac caacaagaat
    19441 taataactga aaaaaacgat cgccttgcag cgtgcgtaaa aaaggcaggc tcatttggca
    19501 ccgtgtttct gggtaaacaa acgaaaggca tccagcgtct cttcactaac atggtgctcc
    19561 atgccttccg cgtcgcgacg ggcgatttcc ggactgacgc ccaacaccaa caagaaattt
    19621 tcgactatct gatgtcgctc gcggctttcc tgcgccagct tctctccttc tgccgttaaa
    19681 aacacgccac gccaggggat catttcaatc agccccatag ttgccagtcg cttaagcatt
    19741 ttagccaccg tcggttgcga aactcccaaa cgagcagcca tgtctacctg acgagcttcc
    19801 cccacttccc tgatcaagtc agaaatcagc tcaacgtaat catcaataag ctcgcgccga
    19861 tgcgcctctc tgacctggcg gaacccttca acgtgctctt cctcgttcac taactgcgtc
    19921 actttttttg ctgttggcgt acctgcgcga cgactcattg tgcttcctca ttatggtgac
    19981 gcatacatag cgttctcatt tcgtgcgcac attgtaaacc agagttgcga aggtacaaaa
    20041 aattaacgtt ttagcaatag ctatataata tagcctgtgc tatatctgta tgtaatgcaa
    20101 tcatccctca aggatcgacg ggattagcaa gtcaggaggt cttatgaatg agttcaagag
    20161 gtgtatgcgc gtgtttagtc attctccctt taaagtacgg ttaatgctgc tctctatgtt
    20221 gtgcgatatg gtcaacaaca aaccgcagca agataaacct tccgataaat agcggcgtcg
    20281 cggtacgccg cttcactcct gctttcatgc aggcacgacg cgttttggtc tgaaaaaccc
    20341 cactttttgt cggatttgca atccccttcg caaaagattt gttcgtcagt agttgacctg
    20401 aactgcggct cgctctatct tcttgcagcc ctgcgtatat tgcggctcgc ggatgcggac
    20461 ccctttcccc tcttcacgca ctcttgcagg tattgaccct tgacgccagg gtaagcacat
    20521 ggcgtttgtt acgatagtgg catatgaatt taaccctcaa agaatcgctt gttacccgta
    20581 gccgggtatt tagcccgtgg actgcgttct actttttaca gtcgctatta attaacctcg
    20641 gcttaggtta ccccttcagt ttgctctaca ccgctgcgtt tacggctatt ttgcttttgc
    20701 tatggcgaac attgcctcgc gtacaaaaag ttctggtcgg tgtcagttcg ctggtggcgg
    20761 cttgttattt cccttttgct caggcctacg gcgcgcctaa tttcaataca ttgctggcat
    20821 tgcactccac caatatggaa gagtcgaccg aaatcctgac gatttttccg tggtacagct
    20881 acctggtcgg cttatttatt tttgcgctcg gcgtaatagc aatcaggcga aaaaaagaga
    20941 atgaaaaagc gcgctggaat accttcgaca gcctgtgtct ggtattcagt gtggcgacat
    21001 tttttgttgc tcccgtgcaa aacctggcct ggggtggcgt atttaaactg aaagatactg
    21061 gctatccggt atttcgtttt gctaaggatg tcatcgtcaa taataacgag gtgattgaag
    21121 agcaagaacg gatggcaaaa ctttccggaa tgaaagatac ctggacggtc actgccgtta
    21181 agccgaagta tcagacctat gtggtggtga tcggtgaaag cgcgcgtcgc gatgccctcg
    21241 gtgcctttgg cggtcactgg gacaataccc cgtttgccag cagcgttaac ggtttgatat
    21301 ttgctgacta cattgccgcc agtggctcca cgcagaaatc gcttggctta acgctcaatc
    21361 gcgtagtcga tggcaaacca cagtttcagg ataactttgt caccctggca aatcgcgcgg
    21421 gcttccagac ctggtggttt tccaaccagg gccaaatcgg cgaatacgat actgctatcg
    21481 ccagcatcgc caaacgtgca gatgaagtgt acttcctgaa agaaggtaat tttgaagcag
    21541 ataaaaacac gaaagacgaa gcgttactgg atatgaccgc tcaagtgctg gcgcaagagc
    21601 actcgcaacc gcagctgatt gttctgcatc tgatgggctc gcatccgcag gcctgcgacc
    21661 ggacaaaagg caaatacgaa acctttgtgc aatcgaaaga aacgtcgtgc tatctctata
    21721 ctatgacgca aacggacgat ttactgcgca agctgtacga tcagttacgc aacagcggca
    21781 gcagcttctc gctggtttac ttttctgacc acggtctggc ctttaaagag cgcggcaaag
    21841 acgtgcaata ccttgcccat gatgataagt atcagcaaaa tttccaggtg ccttttatgg
    21901 tcatttccag cgacgataaa gcgcatcggg tgattaaagc ccgccgctca gccaatgact
    21961 tcttaggctt tttctcgcag tggacgggaa ttaaagcgaa ggaaattaac atcaaatacc
    22021 cgtttatatc tgagaagaaa gccgggccga tatacatcac caacttccag ttacagaagg
    22081 tggattacaa ccatctcgga accgatattt tcgacccgaa gccttaaaaa caaaaatccg
    22141 ccccgagagg cggatttttt atatcaccaa agtgattaga agcggtaacc aacaccggca
    22201 atccaggtgc ctacgtcaac gctacgaata cggctctgct cgtaagagaa gtccagagca
    22261 acgttttcca tcgggttgaa ctgcagacca gcaccgtagg agaaaccgta gtcgctggtg
    22321 tcgtgtttgt aggtcgggta ttcagtggtc tggaatttac cataacccac acccactaca
    22381 ccgtagatgc ttgcccagtc gttaatgcgg taagccggac cagcagtgat gccgtagtac
    22441 tggtttttgt tgtagtcacc agagcttgca gtacggcttt tctcggtgta agtgaatgaa
    22501 ccgatcacac ccagcgggct gttgtcttct tcatagcggt atttcaggtt gaaaccgccc
    22561 attttgttca tttggccctg agcgtcgctc tgtgcgtaac cgccagttac ggtagaagtc
    22621 gcagctacgg aagtacctgc ggtgaaagcc agaactgcgg ccagtgctga aagacatgca
    22681 atttttttca taaccacctc aaatgtgatt caaataagtc ctaagtttta aatatatcaa
    22741 aaattaatgg gaaactcttc gcgatttgtg atgtctaacg ggccatttca tgtaacagaa
    22801 cgtttccata caccgctatc catctaaatt taaatcactt tttcagagaa ctgcgtaagt
    22861 attacgcatg ttttccctgt cattcatcca gattattcct aatcaccaga ctaatgattc
    22921 catcaatcct ggcgcatttt agtcaaaacg ggggaaaatt ttttcaacaa atgctcaacc
    22981 agcattgggt atatccagta cactccacgc tttacttaag tctagatatt tgtgggagaa
    23041 aggatgcctg gttcattacg taaaatgccg gtctggttac caatagtcat attgctcgtt
    23101 gccatggcgt ctattcaagg tggagcttcg ttagctaagt cactttttcc tctggtgggc
    23161 gcaccgggtg tcactgcgct gcgcctggca ttaggaacgc tgatcctcat cgcgttcttt
    23221 aagccatggc gactgcgctt tgccaaagag caacggttac cgctgttgtt ttacggcgtt
    23281 tcgctgggtg ggatgaatta tcttttttat ctctctattc agacggtacc gctgggtatt
    23341 gcggtggcgc tggagttcac cggaccactg gcggtggcgc tgttctcttc tcgtcgcccg
    23401 gtagatttcg tctgggttgt gctggcggtt cttggtctgt ggttcctgct accgctgggg
    23461 caagacgttt cccatgtcga tttaaccggc tgtgcgctgg cactgggggc cggggcttgt
    23521 tgggctattt acattttaag tgggcaacgc gcaggagcgg aacatggccc tgcgacggtg
    23581 gcaattggtt cgttgattgc agcgttaatt ttcgtgccaa ttggagcgct tcaggctggt
    23641 gaagcactct ggcactggtc ggttattcca ttgggtctgg cggtcgctat tctctcgacc
    23701 gctctgcctt attcgctgga aatgattgcc ctcacccgtt tgccaacacg gacatttggt
    23761 acgctgatga gcatggaacc ggcgctggct gccgtttccg ggatgatttt cctcggagaa
    23821 acactgacac ccatacagct gctggcgctc ggcgcaatta t
//
LOCUS       DBFIFM010000057        23799 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.057, whole genome shotgun sequence.
ACCESSION   DBFIFM010000057 DBFIFM010000000
VERSION     DBFIFM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.057"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..3729
                     /gene="yfaL"
                     /locus_tag="N7C74_001962"
     CDS             <1..3729
                     /gene="yfaL"
                     /locus_tag="N7C74_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HGU2431836.1"
                     /translation="EYLSLLPSMIASLFPANGVAAAIDLCQGYNIKASCHASRQSLSG
                     ITQVWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFANNIVSGEYN
                     NGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAIDLRVTNAVFRNNIAN
                     DGKGGAIYTINNDIYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTII
                     NNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPS
                     SAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADN
                     NDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGS
                     TQQTFAHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLAGAQSM
                     ALTGDIVVDAGAVLSLEGDAADLAALQDDPQSIVLNGGMLDLSDFSTWQSGTSYKDGL
                     EVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVIDAGDGQVSLANDNQYLGTTQ
                     IASGTLMVSDNSQLGYTHYNRQVIFTDKPQESVMEITANVDTRSTTTEHGRDIEMRAD
                     GEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSAVRVEEGTL
                     QGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSV
                     ALNASLFNCDGTLVNATDGVTLTGELNTNLETDSLTYLSNVTVNGNLTNTSGAVSLQN
                     GVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEP
                     TSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPS
                     PPDPDPTPDPDPTQDPDPTPDPEPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHA
                     GGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDGEWMLGIVGGY
                     SDNQGDSRSSMTGTRADNQNHGYAVGLTSSWFQHGKQKQGAWLDNWLQYAWFSNDVSE
                     HEDGVDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANRARVSQS
                     QGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDASTISDDAVKQRGEIKV
                     GVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(3870..4592)
                     /gene="ubiG"
                     /locus_tag="N7C74_001963"
     CDS             complement(3870..4592)
                     /gene="ubiG"
                     /locus_tag="N7C74_001963"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HGU2431837.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LSYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            4739..7366
                     /gene="gyrA"
                     /locus_tag="N7C74_001964"
     CDS             4739..7366
                     /gene="gyrA"
                     /locus_tag="N7C74_001964"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HGU2431838.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7574..9202
                     /gene="yfaA"
                     /locus_tag="N7C74_001965"
     CDS             7574..9202
                     /gene="yfaA"
                     /locus_tag="N7C74_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HGU2431839.1"
                     /translation="MALLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIASDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVASARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPVSQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLTSLPKNLEPVFYNAAQTLLMP
                     KLDALSQQPRYVMKLAQMEPGAAWQWLPIIWQPL"
     gene            9199..9822
                     /gene="yfaT"
                     /locus_tag="N7C74_001966"
     CDS             9199..9822
                     /gene="yfaT"
                     /locus_tag="N7C74_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HGU2431840.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9846..14360
                     /locus_tag="N7C74_001967"
     CDS             9846..14360
                     /locus_tag="N7C74_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HGU2431841.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEESKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGKELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGDLPSGGKSFTVNFDKPGNYNLTLRDKDGLILAGLSHAVSGKGSM
                     SHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPSAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAVNSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPSVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREAAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAGKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            14361..16010
                     /gene="yfaQ"
                     /locus_tag="N7C74_001968"
                     /pseudo
     CDS             14361..16010
                     /gene="yfaQ"
                     /locus_tag="N7C74_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
     gene            16015..16791
                     /gene="yfaP"
                     /locus_tag="N7C74_001969"
     CDS             16015..16791
                     /gene="yfaP"
                     /locus_tag="N7C74_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HGU2431842.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDSSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            17066..19915
                     /gene="rcsC"
                     /locus_tag="N7C74_001970"
     CDS             17066..19915
                     /gene="rcsC"
                     /locus_tag="N7C74_001970"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HGU2431843.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKADPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLMIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(20115..20765)
                     /gene="rcsB"
                     /locus_tag="N7C74_001971"
     CDS             complement(20115..20765)
                     /gene="rcsB"
                     /locus_tag="N7C74_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HGU2431844.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(20782..23454)
                     /gene="rcsD"
                     /locus_tag="N7C74_001972"
     CDS             complement(20782..23454)
                     /gene="rcsD"
                     /locus_tag="N7C74_001972"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HGU2431845.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         5794 a         5820 c         6493 g         5692 t
ORIGIN      
        1 gagtatttat ctctactccc gtcaatgatt gcatctcttt tccctgctaa cggtgtcgcg
       61 gcggccattg atttatgcca gggatataat atcaaagcga gttgtcacgc cagcaggcaa
      121 agcctttcag gcattacgca ggtctggagt attgccgatg ggcaatggct ggttttttcg
      181 gatatgacca ataatgccag cggtggggcc gtatttttgc aacaaggagc ggaatttaca
      241 ttatcaccag aaaatgaaac tggaatgact ctgtttgcca ataacatcgt ttcaggagaa
      301 tataataacg gcggggcaat atttgctaaa gaaaactcaa cgctgaatct tacggatgtt
      361 attttttctg gtaacgtcgc aggcggctat ggtggcgcaa tctattcttc tggtactaac
      421 gataccggtg ccatcgattt acgtgtcact aacgccgtgt ttcgcaataa catcgctaat
      481 gacggcaaag gtggtgcaat ttataccatc aataatgata tctatttaag tgatgatgtt
      541 tttaacaata accaggcata tacatcaaca agttacagtg atggcgatgg cggcgcaatc
      601 gatgtcacag ataataatag cgacagcaag catccttcag gttatacgat aataaataac
      661 actgccttta caaataacac tgccgaaggt tatggcgggg cgatatatac caatagcgcg
      721 acggctccct atcttattga tatttctgtt gatgacagct acagccagaa cggaggcgtg
      781 ttagtcgatg agaacaatag cgcagcaggc tatggagatg gtccttcctc tgcggcgggt
      841 ggctttatgt atctcggctt aagtgaagtt acctttgata ttgccgacgg aaaaacgctg
      901 gttattggca atacagagaa tgacggagct gttgactcta ttgctggtac cgggttaatc
      961 accaaaacag gttccggcga tctggtactt aatgcagata acaatgactt tactggtgag
     1021 atgcagattg aaaacggtga agttaccctg ggccgcagca actccctgat gaatgtcggc
     1081 gatacgcatt gccaggacga tccgcaagac tgctacggtc tgacgatagg gagtattgat
     1141 aagtaccaga atcaggcaga gctaaatgtt ggctccaccc aacaaacctt tgcgcactca
     1201 ttgacgggct ttcagaatgg cactttaaat atcgatgctg gtggcaatgt tactgttaat
     1261 caaggcagtt ttgctggcac catcgaaggt gctggtcagc tcaccattgc gcaaaacggc
     1321 agctatgtgc tggcgggggc gcagtcgatg gcgctaaccg gcgatatagt ggtggatgct
     1381 ggtgcggtgc tttcgctgga aggcgacgcg gcagatcttg ccgctctcca ggacgatccg
     1441 cagtcgatcg tgttaaacgg cggtatgctc gatctctctg atttctccac ctggcagagc
     1501 ggtacatcat acaaagatgg ccttgaagtc agtggcagca gcggaacggt tatcggcagt
     1561 caggatgtgg tagatcttgc aggcggaaac gatatgcata tcggcggcga cgggaaagat
     1621 ggcgtctacg tggtgatcga tgcgggtgac gggcaggtca gcctggcaaa tgacaatcaa
     1681 tacctcggca caacgcaaat cgcttccggt acgctgatgg tgagcgacaa ctcgcagctt
     1741 ggatataccc attataaccg ccaggttatc tttaccgata agccacaaga aagcgtgatg
     1801 gagattactg ccaatgtcga tactcgctct acaacgactg agcatgggcg tgatattgaa
     1861 atgcgcgccg acggtgaagt ggcagttgat gcgggggtag acacgcagtg gggcgcactg
     1921 atggctgaca gcagcgggca gcatcaggat gagggtagca cattgactaa aacgggggcg
     1981 ggtacactgg agctgaccgc cagcggtaca acgcagtcag cggtgagagt agaagagggc
     2041 acgctgcaag gtgatgttgc ggatatcttc ccttatgctt cgtcgctatg ggtcggtgac
     2101 ggggcaacgt tcgttactgg cgcggatcag gatattcagt caattgatgc tacttccagc
     2161 ggcactatcg acatcagcga tggtacggtt ttgcgcctga ccgggcagga tacttccgtc
     2221 gcccttaatg cctcactgtt taactgcgat gggacgctgg tgaatgccac cgatggtgtg
     2281 acgttgacag gtgagcttaa taccaacctt gaaactgaca gcctgactta tctttccaac
     2341 gtgacggtta atggcaatct gaccaatacg tccggtgcgg ttagcctgca aaatggcgtc
     2401 gctggcgata cgctgacggt aaacggtgat tataccggcg gcggtacgct actgctcgat
     2461 agcgaattaa acggcgatga ctcggtaagc gatcaattgg tgatgaacgg taatactgct
     2521 ggcaacacaa ctgtggtggt taactccatt acagggattg gtgagccgac atcgacaggc
     2581 attaaagtgg ttgatttcgc agctgatccc acgcagtttc aaaacaatgc gcagttcagt
     2641 ctggcaggca gcggctacgt caatatggga gcgtatgact acacgctggt ggaagataac
     2701 aacgactggt atctgcgatc gcaagaagta acgccgccat cgccacctga tccagacccg
     2761 actcccgatc ctgatcccac gcaggatcct gatccaacac ccgacccgga acctacgcct
     2821 gcttaccagc cggtgttgaa tgccaaagtt ggcggttatc tcaataacct gcgggcggca
     2881 aatcaggcgt ttatgatgga gcgacgcgat cacgcaggtg gcgatggtca gacgctgaat
     2941 ttacgtgtta tcggcggaga ttatcattac acagcagcgg ggcaactggc tcaacatgaa
     3001 gacacttcta cggtgcaact tagcggcgat ctgtttagcg ggcgctgggg cacggatggc
     3061 gagtggatgc ttgggattgt tggtggctac agcgataacc agggcgacag ccgctcgagt
     3121 atgaccggaa ctcgcgccga taaccagaac cacggttatg cggttgggct gacctcaagc
     3181 tggtttcagc acggtaagca gaagcagggg gcctggctgg ataactggtt gcagtacgcg
     3241 tggtttagca atgatgtttc tgaacatgaa gatggcgtgg atcattacca ttcgtcgggg
     3301 attatcgcct cgctggaagc ggggtatcag tggttaccgg ggcgtggtgt ggtgattgaa
     3361 ccgcaggcgc aggtgattta tcagggcgtg cagcaggatg attttaccgc cgctaaccgt
     3421 gcgcgcgtgt cacaatcgca gggtgatgat attcagacgc ggctgggttt acacagcgaa
     3481 tggcgtaccg ctgttcatgt cataccaaca ttagatctga attattatca cgatccccat
     3541 tcgacggaaa ttgaagaaga tgccagcact atcagtgacg atgcggtgaa gcaacggggt
     3601 gaaataaaag tgggagtcac gggcaatatc agtcagcgag tttcgctgcg tggtagcgtg
     3661 gcgtggcaga aagggagtga tgattttgcc cagacggcag ggtttttgtc gatgacggtg
     3721 aaatggtaat cgctgtctga ccatcactat tgctggatgc ggcgtaaacg cctaatcagg
     3781 cctacggaga gcatttgtag gcctgataag acgcggcaag cgtcgcatca ggcattggcg
     3841 cggttgccgg atgaatccaa acaacaacct cacttattct gcgtgtgcag catatagttc
     3901 acatccacgc cggggccgag tttaaaagta ttagtgatcg ggttgtaatg tagcccggtt
     3961 atatgtcgct ctttaaggct tgtctgatct acccagccca gcagttctgc cggtttaata
     4021 aacttcttca catcatgtgt acctttaggc accatgcgca aaatatattc cgcgccgact
     4081 accgccatca gccatgactt gccgttgcgg ttaagcgtcg agaaaaagac atcgccgccc
     4141 ggtttcacca gttgcgcaca ggctctgacc actgactgcg gatcagggac gtgctccagc
     4201 atctccatgc aggtcaccac atcatactgc ccggcatgtt ttgccgcgtg ctcttccacg
     4261 gtttcctgca cgtaatccac ctgaatgccg ctttccagcg cgtgcagctt tgccacctgc
     4321 aatggctcaa agcccatatc cagaccggtc accgtcgcgc cttcgcgcgc catactctcg
     4381 gccagaatgc cgccgccaca accgacatcg agcacctttt tgccaaataa gccgccagca
     4441 cgctcggcaa tatagctcag acgcagcggg ttaatgcggt gcagcggttt gaactcacct
     4501 tccagatccc accagcggga ggcgacggct tcaaatttag cgatctcttc gtggtctacg
     4561 ttatggttta ccggcgattt ttcggcattc attggcactt ctactccgta attggcaaga
     4621 caaacgagta tatcaggcat tggatgtgaa taaagcgtat aggtttacct caatctgcgc
     4681 ggctgtgtta taatttgcga cctttgaatc cgggatacag tagagggata gcggttagat
     4741 gagcgacctt gcgagagaaa ttacaccggt caacattgag gaagagctga agagctccta
     4801 tctggattat gcgatgtcgg tcattgttgg ccgtgcgctg ccagatgtcc gagatggcct
     4861 gaagccggta caccgtcgcg tactttacgc catgaacgta ctaggcaatg actggaacaa
     4921 agcctataaa aaatctgccc gtgtcgttgg tgacgtaatc ggtaaatacc atccccatgg
     4981 tgactcggca gtttatgaca cgatcgtccg tatggcgcag ccattctcgc tgcgttacat
     5041 gctggtagac ggtcagggta acttcggttc catcgacggc gactctgcgg cggcaatgcg
     5101 ttatacggaa atccgtctgg cgaaaattgc ccatgaactg atggccgatc tcgaaaaaga
     5161 gacggtcgat ttcgttgata actatgacgg cacggaaaaa attcccgacg tcatgccaac
     5221 caaaattcct aacctgctgg tgaacggttc ttccggtatc gccgtaggta tggcaaccaa
     5281 catcccgccg cacaacctga cggaagtcat caacggttgt ctggcgtata tcgatgatga
     5341 agacatcagc attgaagggc tgatggaaca catcccgggg ccggacttcc cgacggcggc
     5401 aatcattaac ggtcgtcgcg gtattgaaga agcttaccgt accggtcgcg gcaaggtata
     5461 tatccgcgcc cgtgctgaag tggaagttga cgccaaaacc ggacgtgaaa ccattatcgt
     5521 ccacgaaatt ccgtatcagg taaacaaagc gcgcctgatc gagaagattg cggaactggt
     5581 aaaagaaaaa cgcgtggaag gcatcagcgc gctgcgtgac gagtctgaca aagacggtat
     5641 gcgcatcgtg attgaagtga aacgcgatgc ggtcggtgaa gttgtgctca acaacctcta
     5701 ctcccagacc cagttgcagg tttctttcgg tatcaacatg gtggcattgc accatggtca
     5761 gccaaagatc atgaacctga aagacatcat cgcggcgttt gttcgtcacc gccgtgaagt
     5821 ggtgacccgt cgtactattt tcgaactgcg taaagctcgc gatcgtgctc atatccttga
     5881 agcattagcc gtggcgctgg cgaacatcga cccgatcatc gaactgatcc gtcatgcgcc
     5941 gacgcctgca gaagcgaaaa ctgcgctggt ggctaatccg tggcagctgg gcaacgttgc
     6001 cgcgatgctc gaacgtgctg gcgacgatgc tgcgcgtccg gaatggctgg agccagagtt
     6061 cggcgtgcgt gatggtctgt actacctgac cgaacagcaa gctcaggcaa ttctggatct
     6121 gcgtttgcag aaactgaccg gccttgagca cgaaaaactg ctcgacgaat acaaagagct
     6181 gctggatcag atcgcggaac tgctgcgtat tcttggtagc gccgatcgtc tgatggaagt
     6241 gatccgtgaa gagctggagc tggttcgtga acagttcggt gacaaacgtc gtactgaaat
     6301 caccgccaac agcgcagaca tcaacctgga agatctgatc acccaggaag atgtggtcgt
     6361 gacgctctct caccagggct acgttaaata tcagccgctt tctgaatacg aagcgcagcg
     6421 tcgtggcggg aaaggtaaat ctgccgcacg tattaaagaa gaagacttta tcgaccgact
     6481 gctggtggcg aacacgcacg accatattct gtgcttctcc agccgtggtc gcgtctattc
     6541 gatgaaagtt taccagttgc cggaggccac tcgtggcgcg cgcggtcgtc cgatcgtcaa
     6601 cctgctgccg ctggagcagg acgaacgtat caccgcgatc ctgccggtga ccgagtttga
     6661 agaaggcgtg aaagtcttca tggcgaccgc taacggtact gtgaagaaaa ccgtcctcac
     6721 cgagttcaac cgtctgcgta ccgccggtaa agtggcgatc aaactggttg aaggcgatga
     6781 gctgatcggc gttgacctga ccagcggcga agacgaagta atgctgttct ccgccgaagg
     6841 taaagtggtg cgctttaaag agtcttctgt ccgtgcgatg ggctgcaaca ccaccggtgt
     6901 tcgcggtatt cgtttaggcg aaggtgataa agtcgtctct ctgatcgtgc ctcgtggcga
     6961 tggcgcaatc ctcaccgcaa cgcagaacgg ttatggtaaa cgtaccgcag tggcggaata
     7021 cccgactaag tcgcgtgcga cgaaaggggt tatctccatc aaggttaccg aacgtaacgg
     7081 tttagttgtt ggcgcggtac aggtagatga ctgcgaccag atcatgatga tcaccgatgc
     7141 cggtacgctg gtacgtactc gcgtttcaga aatcagcatc gtgggccgta acacccaggg
     7201 cgtgatcctc atccgtactg cggaagatga aaacgtagtg ggtctgcaac gtgttgctga
     7261 accggttgac gaggaagatc tggataccat cgacggcagt gccgcggaag gggacgatga
     7321 aatcgctccg gaagtggacg ttgacgacga gccagaagaa gaataatttt acttcttcat
     7381 gccaaaaggg agccatctcc cttttttgaa ttgaaaagtc caggctgcaa agtctgggct
     7441 tttgtcgtat tagggcgcgg taaagtttgg ctgtgtccgt aaaaaatggc tggctataca
     7501 caaggaatgt ggcaatgagt ggtgaaaaaa agcgaaaggc tggcggttct atggtcttgt
     7561 aggttttggc gcaatagcac tgctttccgc tggcgtctgg gcgttgcaat atgctggcag
     7621 tgggccagaa aaaacgttgt cgccgctggt ggtgcacaat aatttgcaaa ttgatctcaa
     7681 tgagccggac ctctttctcg acagcgattc tctaagccag cttcccaaag atctcctcac
     7741 cattccgttt ctccacgatg ttctgagtga agatttcgtt ttctattatc agaatcatgc
     7801 cgatcgtctg ggcattgaag gcagcattcg tcgcattgtc tatgaacacg atctcacgct
     7861 gaaagataag ctcttttcgt cactcttaga tcagcccgcg caggcggcgc tatggcacga
     7921 taaacaaggc catctttcac attacatggt gctgatccaa cgcagtggtt taagcaaact
     7981 gctggagcca ttgttatttg ccgctaccag cgacagccag ttaagcaaaa cggaaatcag
     8041 tagcatcaag ataaatagtg aaactattcc tgtttatcag ttgcgctata acggcaataa
     8101 cgccctgatg ttcgcgacat atcaggacaa gatgctggtg ttttccagca cggatatgct
     8161 gtttaaagat gatcagcagg ataccgaagc cacggcgatc gcaagtgatt tgttgagcgg
     8221 caaaaaacgc tggcaagcca gctttggcct ggaagagcgt gctgccgaaa aaacgccagt
     8281 acgccagcgc atcgtggcca gcgccaggtt gctgggtttt ggctaccagc ggttaatgcc
     8341 ttcttttgct ggcgtacgct tcgaaatggg taacgacggc tggcacagtt ttgtggcgtt
     8401 aaatgatgaa tccgccagcg tagatgccag tttcgatttt acgccggtct ggaacagtat
     8461 gcctgccggg gccagcttct gtgtggcggt gccgtattca cacggtattg ccgaagagat
     8521 gctttcgcac atcagccagg aaaacgacaa gttgaatggg gcgttggacg gtgccgcggg
     8581 gctgtgctgg tatgaagact caaaattgca aaccccgctg tttgtcggtc agtttgatgg
     8641 cactgccgaa caggcgcaat tgccagggaa actgtttacg caaaatattg gtgcgcacga
     8701 aagcaaagcg ccagaaggtg ttttgccggt aagccagact cagcagggcg aagcgcaaat
     8761 ctggcgtcgc gaagtgagtt cccgatacgg tcagtatccg aaagcgcagg cggcgcaacc
     8821 cgatcaatta atgtcggatt attttttccg cgtgtcgctg gcgatgcaaa acaaaacgct
     8881 gcttttctcc ctcgatgaca cgctggttaa taacgccctg caaacactga ataaaacccg
     8941 cccggcaatg gtggatgtaa tacccactga tggcatcgtt ccgctctata tcaatccaca
     9001 aggcgtggcg aaactgctgc gtaacgaaac gttgaccagt ctgccgaaga atctcgaacc
     9061 ggttttttat aacgccgcac aaactttatt aatgccgaag ctggacgctt tatctcaaca
     9121 accgcgttat gtcatgaagc tggcccagat ggaacccggt gccgcctggc agtggctacc
     9181 cataatctgg cagccgctat gaggcacggg ctgctggcgc tgatttgctg gctgtgttgt
     9241 gttgttgccc atagcgaaat gctgaatgtc gaacaatccg ggctgtttcg cgcctggttt
     9301 gtacgcattg cacaagaaca gctccgccag gggccaagtc cacgctggta tcagcaggat
     9361 tgtgcgggcc tggtgcgatt tgcggcgaac gagacgctga aagttcacga cagtaaatgg
     9421 ctaaaaagta acggtttatc tagccagtat ttgccgccag agatgacgct aacacctgaa
     9481 cagcgtcaac tggcgcaaaa ctggaatcag gggaacggga aaaccggccc ctacgtgacc
     9541 gcgattaatt tgattcagta caacagccag tttattggcc aggacataaa ccaggcgctg
     9601 cctggcgata tgattttttt cgatcagggc gatgcccagc acttaatggt ctggatgggg
     9661 cgttacgtca tctaccacac cggaagcgcc acgaaaactg acaacggaat gcgcgcagtc
     9721 agtctgcaac aacttatgac atggaaggac acccgatgga tacccaacga ttccaatccc
     9781 aatttcattg gcatttatcg tttaaatttt ctggcgcgat agcagcgtgc ttgtccctgt
     9841 ctcttgtggg aacagggctt gctaatgctg atgattcgct tccttccagt aactatgcgc
     9901 cgcccgccgg gggaacattc tttttgcttg ctgacagcag ttttagcagc agtgaagagt
     9961 cgaaagtgcg actggaagcg ccggggcgtg attatcggcg ctatcagatg gaagagtacg
    10021 gcggcgtgga cgttcgcctg tatcgtattc ctgacccgat ggcatttttg cgccagcaga
    10081 aaaacctgca tcgcattgtg gtgcaaccgc aatatctggg cgacgggctg aacaatacgc
    10141 tgacctggct gtgggataac tggtacggca aatctcgccg cgtgatgcag cgtactttct
    10201 cttctcagtc acggcagaat gtgactcagg cattacccga attacagctc ggcaatgcca
    10261 ttattaaacc ttcccgttat gtacagaaca accagttttc cccgctgaaa aaatatcccc
    10321 tggtggaaca gttccgttat ccactatggc aggctaaacc ggtcgagccg cagcaagggg
    10381 taaaactgga aggcgcatcc agcaatttca tctcgccgca gccgggtaac atttatattc
    10441 ctctcggcca acaagagccg ggactgtacc tcgtcgaggc gatggttggt gggtatcggg
    10501 cgacgacggt ggtgtttgtt tccgataccg tggcgcttag caaagtgtca ggcaaagagc
    10561 ttctggtgtg gaccgcgggt aaaaaacagg gtgaagcgaa gcccggctca gagatcttgt
    10621 ggactgacgg tcttggcgtg atgacccgcg gtgtgaccga tgacagcggt accttgcagt
    10681 tacaacatat atcgccagaa cgttcataca ttctgggtaa ggatgctgaa ggcggcgttt
    10741 ttgtctccga gaacttcttc tacgaaagcg aaatctacaa cacccgcttg tatattttta
    10801 ccgatcgccc gctatatcgc gcaggcgatc gtgtcgatgt taaagtgatg ggccgcgagt
    10861 tccacgatcc gttgcattca tcccccatcg tcagcgcccc ggcgaagctt tcggtgctgg
    10921 acgctaacgg cagtctgttg caaaccgtcg atgtcacgct ggatgcgcgc aatggcgggc
    10981 agggaagttt ccgcctgcca gaaaatgccg ttgccggagg ttatgagtta cgtcttgctt
    11041 accgcaatca ggtctatagc agcagttttc gcgtggcaaa ctacatcaag ccacatttcg
    11101 agattggttt agctctcgac aaaaaagagt tcaaaactgg cgaagcggtc agcggcaaac
    11161 tgcaacttct ctacccggat ggcgagccgg taaaaaatgc ccgcgtgcag ttaagtttgc
    11221 gcgctcagca attatcaatg gtcggtaacg atttgcgtta tgccggacgt ttccccgtgt
    11281 cgctggaagg cagcgaaacg gtgtccgacg ccagcggtca tgtggcgtta aatctccccg
    11341 ccgccgataa accgagccgc tatttgttaa ccgtctccgc cagtgacggc gcggcgtatc
    11401 gcgtcaccac caccaaagag atcctcattg aacgcggcct ggcgcattac tctttaagta
    11461 ccgccgcaca atacagtaat agcggcgagt cggttgtgtt ccgttatgcc gcgctggaat
    11521 cttcaaaaca ggttcctgtt acgtatgaat ggttgcgtct cgaagaccgc acgagccata
    11581 gcggagatct accgtcaggc ggcaaatcct ttaccgtcaa tttcgataaa cctggcaact
    11641 acaatctgac gttacgcgat aaagacggct taattctcgc cgggttaagc catgccgtca
    11701 gcggtaaggg cagtatgtcg catactggta cggtagatat cgtggcagat aaaacgctgt
    11761 accagcctgg cgaaaccgcg aagatgctga ttaccttccc ggagccaatt gatgaagcat
    11821 tattgacgct ggaacgcgat cgcgttgaac agcagtcgct gctatcgcat ccggcaaact
    11881 ggctgacgtt acaacgttta aacgatactc aatatgaagc ccgtgttcca gtgagcaatt
    11941 cctttgcgcc taacatcact ttttcggtgc tgtatacccg taacggtcag tacagttttc
    12001 agaacgccgg gatcaaagtt gccgttcccc agctggatat ccgggtgaaa acggacaaaa
    12061 cccattacca gcctggtgaa ctggtcaatg tcgaattaac ctcgtcgctg aaaggtaaac
    12121 ctgtttctgc gcagctaacg gtaggcgtgg tcgatgaaat gatctacgcg ctgcaaccag
    12181 aaatcgcgcc gaatatcggc aaatttttct atccgctggg gcgtaacaat gtgcgtacca
    12241 gctccagtct gtcgtttatc agctacgacc aggcactctc cagcgagccg gttgcgcctg
    12301 gcgcaactaa ccgcagcgag cggcgagtaa aaatgcttga acgtccacgg cgtgaagagg
    12361 tggataccgc ggcatggatg ccgtcactca caaccgataa acaaggcaaa gcgtatttca
    12421 cgttcctgat gcctgattcg ttaacccgct ggcgtatcac cgcgcgtggg atgaacggcg
    12481 acgggctggt cgggcagggg cgtgcttatc tgcgttcgga aaaaaatctc tacatgaagt
    12541 ggagtatgcc aacggtgtat cgcgtgggcg acaaaccgtc ggcaggactg tttatcttca
    12601 gtcagcagga taacgaaccg gtggcgctgg tgactaaatt tgcaggcgct gagatgcgcc
    12661 agacgctgac gctgcacaaa ggggcgaatt atatttcgct ggcgcagaac attcagcaat
    12721 ctggcttgtt aagcgcagaa ctgcaacaaa atgggcaagt gcaggacagc attagcacaa
    12781 aactgtcttt tgtggataac agctggcccg ttgaacagca gaaaaatgtc atgctcggcg
    12841 gtggcgataa cgcgctgatg ttgcccgagc aggcgagcaa tatccggcta caaagtagtg
    12901 aaacgccgca ggagattttc cgcaacaatc ttgatgcgtt agtcgatgaa ccgtgggggg
    12961 gggtgatcaa caccggtagc cgtctgatcc cgctcagtct cgcctggcgt tcgcttgccg
    13021 atcatcaaag tgccgccgct aacgacattc gtcagatgat tcaggataac cgtctgcggc
    13081 tgatgcaact ggcggggccc ggagcgcgct ttacctggtg gggtgaagat ggcaatggtg
    13141 acgccttcct tacggcatgg gcatggtacg ccgactggca ggccagccag gcgctcggcg
    13201 taacgcaaca accggaatac tggcagcata tgctcgacag ttatgccgag caggcagata
    13261 acatgccgtt attgcatcgg gcgctggtgc tggcgtgggc acaggagatg aatctgccgt
    13321 gcaaaacgtt gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact aaaactgaag
    13381 atttctctga ggaagacacc cgcgatatca atgacagcct gatcctcgat acaccggaat
    13441 ctccactggc agatgcggtg gcaaacgtct taaccatgac gttgctgaaa aaagcgcagt
    13501 tgaagtccac ggtgatgcca caggttcagc aatatgcgtg ggataaagcg gtaaacagca
    13561 atcagccgct ggcacacacg gttgtgctgc tcaatagcgg gggcgacgct acccaggcgg
    13621 ctgctatttt aagtggtttg accgctgagc aatctactat tgagcgcgcg ctggccatga
    13681 actggctggc gaaatatatg gcgacaatgc cttcggttgt gttgcctgcg cctgcgggcg
    13741 catgggccaa acataagtta actggagggg gcgaatactg gcgttgggtt ggtcagggcg
    13801 tgccggacat tctctctttt ggtgacgaat tatcgccgca aaatgtgcag gtccgctggc
    13861 gtgaagcggc aaaaacggct caacaaagta acattccggt gaccgttgaa cgccaattgt
    13921 atcggcttat ccctggtgaa gaagagatga gctttactct gcaaccggtg accagcaatg
    13981 agattgacag cgatgcgctg tatctcgatg aaattacgct taccagcgag caggatgcag
    14041 ttctgcgcta tggtcaggtg gaagtaccgc tcccgccggg agccgacgtt gagcgcacaa
    14101 catggggcat ttcagtcaat aaacccaacg ctggaaaaca gcaggggcaa ttgctggaaa
    14161 aagcgcgaaa tgaaatgggc gaactggcct atatggtgcc ggtgaaagaa ctgacgggaa
    14221 cggtcacttt ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt ctgcctcctg
    14281 ctcgttatgt gcgttcctat gcacctgcac agcaaagtgt tgcggcaggg agcgaatgga
    14341 ccgggatgca ggtgaaataa gtgaactggc gcagaatcgt ctggctattg gcgttggtta
    14401 ctctgccaac gcttgcggag gagacgccgt tacaactggc gctgcgcggt gcgcagcacg
    14461 atcaactgta tcagctgtcg tcatcaggcg tgaccaaagt ctcagcatta cctgacacgc
    14521 tcaccacgcc gttagggagt ttatggaaac tgtatgttta cgcctggctg gaggataccc
    14581 atcaaccgga gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt tattgttgtc
    14641 aggctgggga gagcataacg cgcgataccg cgctggtacg ttcctgtggg ctctattttg
    14701 cgccgcagcg gttgcatatt ggggcagatg tgtgggggca atactggcaa cagcgtcagg
    14761 cacctgcgtg gttagcgtct ctttctacgc tgaagccaga aacctcggta acggttaaat
    14821 cgctgcttga ttcattagcg acgttacctg cgcaaaacaa agctcaggaa gtcttactgg
    14881 atgtggtgct ggacgaggcg aaaatcggtg tcgcatctat gctcggtagt cgggtacggg
    14941 tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgtcagggt ggttttgctg
    15001 gctggttaac cgatggtaca ccgctgtggg tgactggcag tggcaccagc aaaacggtac
    15061 tgacccgtta tgcgacggtg cttaatcggg ttttgccagt gccgacgcaa gttgccagtg
    15121 ggcaatgtgt agaggtggag ctatttgcgc gctagccgct gaagaaaatc acagcggaga
    15181 agagtacgac ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg acgtttgcca
    15241 atggtaacca cattacgttt gtcagccacg gtgagacgac cttactgacc gaaaagggca
    15301 aactgaaatt gcagtcgcat ctcgatcgtg aagagtacgt tgccagagtt ctggatcgcg
    15361 aggcgaaaag tacgccacct gaggcagcga aagccatgac tgtggcgata cgcacttttt
    15421 tacagcaaaa cgccaatcgg gaaggcgatt gcctgaccat ccccgacagc agcgccacgc
    15481 agcgtgtttc tgcttcgcca gctaccacgg gggcgcgaac gatgaccgtc tggacgcagg
    15541 atctcatcta tgcgggcgat ccggttcatt atcacggcag tcgcgctacc gaagggacgc
    15601 tttcctggcg gcaagccatg gcccaggcgg ggaagggaga gcgttacgac cagatactcg
    15661 cgtttgctta tcccgacaac agccttagcc gctggggagc gccacgcaca acctgtcagt
    15721 tattacccaa agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg cgtatattac
    15781 aaggcgagac ggggtacaac gaaccagacg tgtttgcggt ctgtcgttta gtctcaggtt
    15841 tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt acgcttcagg
    15901 atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat cccaccgggc
    15961 ttgatgagaa ctatatcgaa acgctgaccc gacaattatt aatggactaa tgctatgcga
    16021 aagatttttc ttccgttgtt actggtggcg ctttcgcctg tcgctcacag tgaaggtgtg
    16081 caggaagtcg agattgacgc accgctctct ggctggcatc cggcagaggg cgaagatgcc
    16141 agtttttcac aatcgatcaa ttacccggcg tcgtccgtca acatggcaga tgatcaaaat
    16201 atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa agttcagcag
    16261 ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga tagttcattt
    16321 gcacgccctt atattttcac tgaaggcagc aacagcgtgc aggtcatcag cccggatggg
    16381 caaagccgac aaaaaatgca gttttactca acgccgggta caggaacgat tcgtgcacgt
    16441 ttacggctgg ttctctcgtg ggatacggac aataccgacc tcgatcttca cgttgtcacg
    16501 cccgatggcg aacacgcctg gtacggtaac accgtgctga aaaacagtgg tgcattggat
    16561 atggatgtca cgacggggta cggacccgag attttcgcca tgccagcacc agttcacggg
    16621 cgctatcagg tgtatatcaa ctactatggt gggcgcagcg aaacggaatt aaccaccgcc
    16681 caactgacgc tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac atttattgta
    16741 ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt ttgactggtg atccatccct
    16801 tcacacaacc tggctctttc ttttttcaga taagactgct gggcgatctg ccgacgccag
    16861 gatataacca ttaacaagcg tcggcagatt ggtcatttct tatttttcgt actgagaact
    16921 gtccatttac tccggctttc ctggttaagg caatggtttc taagcgatac gcattattgc
    16981 aatggtgaca gaacattgcg gtaacacgct tttaccgcta ccttaaccac actccatcgg
    17041 tcacctgagg cggagcttcg cccctttgaa ataccttgct tcttttcgta caaccctgaa
    17101 agcctcgcgc tacatgttca gagcattggc gttagtgctc tggctgttga ttgctttttc
    17161 atccgttttt tacatcgtta atgcgttaca tcagcgagaa tcggaaattc gtcaggaatt
    17221 taatctgagt tccgatcagg ctcagcgctt tattcaacgc acctctgatg tgatgaaaga
    17281 gctgaagtac atcgccgaaa atcgcttatc ggcagaaaac ggtgtgcttt ccccgcgtgg
    17341 acgagaaacg caggcggatg tgcctgcgtt tgaaccgctg tttgccgact ccgattgttc
    17401 agcaatgagt aacacctggc gaggttctct ggagtcattg gcgtggttta tgcgctactg
    17461 gcgcgataat ttttctgcgg cttacgatct caaccgggta tttttaatcg gcagcgataa
    17521 cctctgcatg gccaatttcg gtctgcgtga tatgccagtg gaacgcgata ccgcgttgaa
    17581 agctttgcat gaacgcatca ataaatatcg aaatgcacca caagatgata gcggcagtaa
    17641 cctctactgg atcagcgaag gtccgcgccc tggcgtcggg tatttttacg cgttgacgcc
    17701 agtttatctg gcgaaccggt tgcaggcgct tttgggtgtc gagcagacca tccggatgga
    17761 gaactttttc ttaccgggta cgttgccgat gggggttacc attcttgatg aaaatggtca
    17821 taccctgatt tcgcttaccg ggccagaaag caaaattaag gccgatcctc gctggatgca
    17881 ggaacgctcc tggtttggct atacggaagg gttccgggag ctggtgctga agaaaaatct
    17941 gccaccctca tcgctcagca tcgtgtattc ggtgccggtt gataaggtgc tggaacgcat
    18001 tcgcatgttg atccttaacg caattttgct gaatgtgctt gccggagctg cattgtttac
    18061 tctcgcgcgg atgtacgagc gacgtatttt cattccggcg gaaagcgacg ccctgcgact
    18121 ggaagaacat gagcagttca atcgcaagat tgtcgcctcc gcgccagtgg gtatctgcat
    18181 tttgcgtacc gctgatggcg tcaatatttt aagtaacgaa ctggcgcata cctatctcaa
    18241 tatgcttacg catgaggacc gccaacgact gacacaaatt atctgtgggc agcaggtcaa
    18301 ttttgttgat gtcctgacca gcaacaatac caatctgcaa atcagcttcg tccattcgcg
    18361 ctatcgtaat gaaaacgtgg ccatttgtgt gctggtggat gtttcttcgc gcgtgaagat
    18421 ggaagagtcg ttgcaggaga tggcacaagc agcggaacag gcgagccagt caaaatcgat
    18481 gttccttgcc accgtcagtc atgagctgcg aacgccgctg tatggcatta tcggtaacct
    18541 ggatctgttg caaaccaaag agttaccgaa aggcgtcgat cgtctggtga cggcaatgaa
    18601 caactcttcc agcctgttgt tgaaaattat cagcgatatt ctcgatttct cgaagattga
    18661 atcggaacag ttgatgatcg aaccgcgtga gttttcaccg cgtgaagtga tgaaccacat
    18721 caccgccaac tatttaccgc tggtggtacg caagcagtta ggcttgtact gctttattga
    18781 accggatgtg ccagtggcct taaatggcga cccgatgcgt ttacagcagg tcatctccaa
    18841 cctgttgagt aacgccataa aattcaccga taccggctgt atagttttgc atgtccgcgc
    18901 tgatggcgat tatctctcta tccgtgttcg cgataccggc gtggggattc cggcgaaaga
    18961 agtggtgcgc ttgtttgatc ccttcttcca ggtcggaacg ggcgtacagc gaaacttcca
    19021 ggggaccggt ctgggtctgg cgatttgtga aaaactgatc agcatgatgg acggcgatat
    19081 ctcggtagat tcagaaccgg gaatgggcag ccagtttacc gtgcgtattc cgttgtacgg
    19141 cgctcagtac ccgcagaaaa aaggcgtgga agggttgagt ggtaaacgct gctggctggc
    19201 ggtccgcaat gcgtcgctct gtcagttcct ggaaaccagt ttgcagcgca gcggcatcgt
    19261 cgtaacaaca tacgaagggc aggaaccgac tcccgaagat gtgttaatca ctgacgaggt
    19321 agtgagtaaa aaatggcagg gcagagcggt agtgaccttc tgtcgtcgac atattggtat
    19381 tccgctggag aaagcgccag gggagtgggt acacagtgtg gctgctccgc atgagctacc
    19441 ggcattgttg gcgcgtattt atttgatcga gatggagagc gacgatcctg ctaacgctct
    19501 gccgtcgacg gacaaagcgg tcagcgataa tgacgatatg atgattctgg tcgtggatga
    19561 tcatccgatt aaccggcgtt tgctggcaga tcagttggga tcgttgggct atcaatgtaa
    19621 aaccgcgaat gatggcgtcg atgcgcttaa tgtacttagc aagaatcata ttgatatcgt
    19681 gcttagcgac gtcaacatgc caaatatgga tggttaccgc ttgacacaac gcattcgtca
    19741 gttgggactg acgttgccgg taatcggagt aacggctaat gcgttggctg aagagaagca
    19801 gcggtgtctg gagtccggta tggacagctg cctgtcgaag ccagtaacgc tggatgttat
    19861 aaaacagacg ctgacggtat atgccgagag ggtcaggaaa tcgcgggaat cgtaggacgg
    19921 ataaggcgtt tacgccgcat ctggcattca gtgcaaatgc cagatgcgac gctgacgcgt
    19981 cttatctggc ctactttaat gtaatctgtt gaaataatgg gaatcgtagg acggataagg
    20041 cgtttacgcc gcatccggca atcggtgcaa atgccagata agacactaac gcgtcttatc
    20101 tggcctacag atgattagtc tttatctgcc ggacttaacg ttactgaaga gagataattc
    20161 agcagggcga tatcgttctc gacacccagc ttcatcatcg cagatttctt ctggctactg
    20221 atggttttaa tactgcggtt cagcttttta gcgatctcgg tcaccaggaa gccttccgca
    20281 aacaggcgca gaacttcact ctcttttggc gagagacgtt tgtcaccgta acctccagca
    20341 ctgatttttt ccaacaggcg agaaacgctt tccggggtaa atttctttcc tttttgcagc
    20401 gctgcgaggg ctttcggcag atcggtcggt gcaccttgtt tcagcacgat cccttcgata
    20461 tccagatcca ataccgcact aagaatcgcc gggttgttgt tcatggtcag aacaataatc
    20521 gacaggcttg ggaaatggcg cttgatgtac ttaattaagg taatgccatc gccgtactta
    20581 tcgccaggca tggagagatc ggtaatcaac acatgcgcat ccagtttcgg caggttgttg
    20641 atcagtgctg tagagtcttc aaattcgccg acaacattca cccactcaat ttgctcaagt
    20701 gatttgcgaa taccgaacaa gactatcgga tggtcatcgg caataattac gttcatattg
    20761 ttcatgtaat aggctacctt gctacagcaa gctcttgaca taactgtcaa tgtcgctgat
    20821 gtatttttct attcctggaa catccttctc acgaatcaga tgttccagcg tttcacataa
    20881 ctgcttgccg ggtaccagat ttagcatggc aaatacgcct ttaagacgat gagccgtttg
    20941 ggctaacgca gcaaagtcac tggttgctgc ttcagtatac agcctcttaa catcatccgg
    21001 tactgtgtct acaaagagcg cataatagcc gctggcatgg agttgcgcat tttcatctcc
    21061 gcccagaggc gattctgtca cctcttcctg cgccagttgc acttcaatta attgtaagac
    21121 cgcttcctgc atagcgttgc tcatattgaa gttgacgcac aattgaccag gcccaatttc
    21181 ccgtacgcca gactcatcat cgcttaaaag caagccagag gcagtaagat tagacggatt
    21241 atccgttaaa aagatatcat aatcttgact aattaatctt tcatcgggtg tgatacaggt
    21301 tgcaccccaa ttttctaact ggcgagtgac aatattccga atttctgccg aagtaacatc
    21361 caccattacg cagacatcat ccagtaaacg ctcttcttcc tcttcaactt ccgggtcagc
    21421 tgcgagcatt ttgatatgca cagagtagcg tgtaccaagc ccatcccgcg ttttgatgtt
    21481 taaatgaccg cccagtttac gtgccagttg atcgctcagc cagaatgcca gcgggtccgc
    21541 cttgccatag cgatcgtttt gggtctggtt gataaacggg aagtgcaaat tatccatttc
    21601 atgaatgctt acgccttctc cggtgtccag aatgcggaac gtcaggcggt cttcggagga
    21661 ctcatcctga tcaacctcaa gggtgatttt tcccaattgc gttgaggtca cggcatattg
    21721 catcagtagc agcaaaatac gtcgtaatgc atcgcgatcg ccgcggcgca tatcgtgtgc
    21781 tttcagatga ttgttaatca gcagttgcag acctttacgc ttgatggcag gcaacactga
    21841 aggcacaact tcatcaatta aatcctgcac ggagaacagc accgtctcac ttttccagct
    21901 atcgtccgca agcatgttcg ctaactgtat ttcatcgacc aatcgcacca gcacatctgc
    21961 ctgattcgcc agttgtttgc tttccggggc gttgagttta gccgcgctct ccgccaggga
    22021 ctgtgcgggt tctttcagcg catcgccaat gtttttcata aaggtcatcc gcccctgctg
    22081 gtttttctca tacagacgct gcgcctgctt gagtttcttg tttaccagca cttcgcgatc
    22141 ctgatcgcga ataatgaaaa tttgtgtgcg cggcgcaacc tggctgcgga acatgcggat
    22201 ctcatacagc tcgttattga tcgtcgcctg aataatcccc tgatgctgtt ccgccatggt
    22261 ggtgatgttt tgcagattca aatgcggcag caaatgatcg gcaattttgt tacttatgac
    22321 agtgcggttc gattcctgat cgtgaaccag caggccgagc ggcagcagtg agactatctc
    22381 ttcattgatt gcccgtaaaa tgcgcaattc gttattgacc gccgtgttgg gtagactttc
    22441 tgtactgcga ctggagaaat ggcggaatgt ggtatagcca aataacgcca gcgccagcaa
    22501 accgatgttc agcagcagtg gcagcagaat gttttgcaac gtatccagca ataaggtgcc
    22561 ataaggaacc tgccagacca ggcgcatatc ggtagagttg agtgccgagg agatttcaat
    22621 cttcgtactg ttaaagtgga tactgacgct atccgttcct tctttctcat tatcattgtt
    22681 tcccgttgcc gtcgcgtctg gctcaaggcg gaaactgtcc agcggcatac ccggtgggat
    22741 caaatcatta atcggcagat caaaagccac gaccgttgcc agatgtcccg gctggttgaa
    22801 cgtggtacgc aaggtaaagt aatgaccgtt ctgccaggcc aggcggcgca tgttagaaaa
    22861 actttcgcgt tcatcgaggg cgttggcctg ttgcaacatc tctgcacgac gtgaatcaac
    22921 aatgtcactg acggtcgatt ctttaaatcc ggaggtgaga tctttgaggg gcagggttga
    22981 gatcagcacc agactgttat cctgaccatt caggtaatac attgaccacg gtacattttc
    23041 tgcgccccac aatgtgtcca gataggtgga catccgttga gtcatctcaa gcgttgagtt
    23101 gtcgtgagag ccaaagatta acgcttccgt tttgcggcgc ggtttttcca gatagtagac
    23161 atcctgtttc aggcgcgtct cttgtaaacc ttcgccggag gagggggagg tcgtcgcggc
    23221 aatgttgtcg tagatctgcc aggtcacgta acgccaggta tcgacgcgct tttggatagc
    23281 gtgggtaatg tcgacaattt ggtaactttt atctttcagc caggcgttaa cggcgctttg
    23341 taccattaca cccatcgtca ccagtaacac aatgatcaac agtaaaaaga agcgggtaat
    23401 gctccccggt aggagtgaaa agcgggtcgt ggccgttgtc tctttctgac gcattcgtgt
    23461 ttatgacctg ttaaaacttc gcgaagtgat agggcagtat aaagggtaca gtgtgaaatt
    23521 ccagactctt acctgattga tgattctcta ttctcccgtg atatttatca tcctgcattg
    23581 ggggaaatag atgtacaagc gccattttgc tgtacaaata acctacaaaa agcccaacga
    23641 aaataattgc aaataaacaa agattgctgg aaattatgct gatgttaatt atttgtgaaa
    23701 tagttaacaa gcgttatagt ttttctgtgg tagcacagaa taatgaaaag tgtgtaaaga
    23761 agggtaaaaa aaaccggatg cgaggcatcc ggttgaaat
//
LOCUS       DBFIFM010000058        23765 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.058, whole genome shotgun sequence.
ACCESSION   DBFIFM010000058 DBFIFM010000000
VERSION     DBFIFM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.058"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..1234
                     /locus_tag="N7C74_001973"
     CDS             <1..1234
                     /locus_tag="N7C74_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGU2431846.1"
                     /translation="VKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILS
                     LAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGK
                     DNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINY
                     TKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISR
                     GIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKD
                     FCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIHPDDILARLEGEVFGLLFTE
                     LNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADK
                     ALREAKSEGGNKVIIHHI"
     gene            1415..2905
                     /gene="dgcJ"
                     /locus_tag="N7C74_001974"
     CDS             1415..2905
                     /gene="dgcJ"
                     /locus_tag="N7C74_001974"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HGU2431847.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSILQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2948..3451
                     /gene="yeaK"
                     /locus_tag="N7C74_001975"
     CDS             2948..3451
                     /gene="yeaK"
                     /locus_tag="N7C74_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HGU2431848.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3452..3556)
                     /gene="yoaI"
                     /locus_tag="N7C74_001976"
     CDS             complement(3452..3556)
                     /gene="yoaI"
                     /locus_tag="N7C74_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HGU2431849.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3726..4172
                     /gene="yeaL"
                     /locus_tag="N7C74_001977"
     CDS             3726..4172
                     /gene="yeaL"
                     /locus_tag="N7C74_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HGU2431850.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIATGLVSLIVGKQ"
     gene            complement(4129..4950)
                     /gene="nimR"
                     /locus_tag="N7C74_001978"
     CDS             complement(4129..4950)
                     /gene="nimR"
                     /locus_tag="N7C74_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HGU2431851.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     YTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGALGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPWQ"
     gene            5047..6228
                     /gene="nimT"
                     /locus_tag="N7C74_001979"
     CDS             5047..6228
                     /gene="nimT"
                     /locus_tag="N7C74_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HGU2431852.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6283..6630
                     /gene="yeaO"
                     /locus_tag="N7C74_001980"
     CDS             6283..6630
                     /gene="yeaO"
                     /locus_tag="N7C74_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HGU2431853.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6652..6906)
                     /gene="yoaF"
                     /locus_tag="N7C74_001981"
     CDS             complement(6652..6906)
                     /gene="yoaF"
                     /locus_tag="N7C74_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HGU2431854.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRLDHPAGEK"
     gene            7089..8114
                     /gene="dgcP"
                     /locus_tag="N7C74_001982"
     CDS             7089..8114
                     /gene="dgcP"
                     /locus_tag="N7C74_001982"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HGU2431855.1"
                     /translation="MSDQIIARISQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNQKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHS"
     gene            8147..8245
                     /gene="yoaK"
                     /locus_tag="N7C74_001983"
     CDS             8147..8245
                     /gene="yoaK"
                     /locus_tag="N7C74_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HGU2431856.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8248..8322
                     /gene="yoaJ"
                     /locus_tag="N7C74_001984"
     CDS             8248..8322
                     /gene="yoaJ"
                     /locus_tag="N7C74_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HGU2431857.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8381..8629)
                     /gene="yeaQ"
                     /locus_tag="N7C74_001985"
     CDS             complement(8381..8629)
                     /gene="yeaQ"
                     /locus_tag="N7C74_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGU2431858.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8776..8958)
                     /gene="yoaG"
                     /locus_tag="N7C74_001986"
     CDS             complement(8776..8958)
                     /gene="yoaG"
                     /locus_tag="N7C74_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HGU2431859.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(8962..9321)
                     /gene="yeaR"
                     /locus_tag="N7C74_001987"
     CDS             complement(8962..9321)
                     /gene="yeaR"
                     /locus_tag="N7C74_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HGU2431860.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9494..10132)
                     /gene="leuE"
                     /locus_tag="N7C74_001988"
     CDS             complement(9494..10132)
                     /gene="leuE"
                     /locus_tag="N7C74_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HGU2431861.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGMATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10259..11182)
                     /gene="dmlR"
                     /locus_tag="N7C74_001989"
     CDS             complement(10259..11182)
                     /gene="dmlR"
                     /locus_tag="N7C74_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HGU2431862.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            11285..12370
                     /gene="dmlA"
                     /locus_tag="N7C74_001990"
     CDS             11285..12370
                     /gene="dmlA"
                     /locus_tag="N7C74_001990"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HGU2431863.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            12621..14231
                     /gene="yeaV"
                     /locus_tag="N7C74_001991"
     CDS             12621..14231
                     /gene="yeaV"
                     /locus_tag="N7C74_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HGU2431864.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGGAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            14263..15387
                     /gene="yeaW"
                     /locus_tag="N7C74_001992"
     CDS             14263..15387
                     /gene="yeaW"
                     /locus_tag="N7C74_001992"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HGU2431865.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            15443..16408
                     /gene="yeaX"
                     /locus_tag="N7C74_001993"
     CDS             15443..16408
                     /gene="yeaX"
                     /locus_tag="N7C74_001993"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HGU2431866.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTPVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(16462..17577)
                     /gene="rnd"
                     /locus_tag="N7C74_001994"
     CDS             complement(16462..17577)
                     /gene="rnd"
                     /locus_tag="N7C74_001994"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HGU2431867.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(17659..19344)
                     /gene="fadD"
                     /locus_tag="N7C74_001995"
     CDS             complement(17659..19344)
                     /gene="fadD"
                     /locus_tag="N7C74_001995"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HGU2431868.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTLVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVSPGQPGELCVRGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(19549..20130)
                     /gene="yeaY"
                     /locus_tag="N7C74_001996"
     CDS             complement(19549..20130)
                     /gene="yeaY"
                     /locus_tag="N7C74_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HGU2431869.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQATGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGHGGWGWYNPGPARVQTVVTE"
     gene            complement(20170..20865)
                     /gene="tsaB"
                     /locus_tag="N7C74_001997"
     CDS             complement(20170..20865)
                     /gene="tsaB"
                     /locus_tag="N7C74_001997"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HGU2431870.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(20923..22833)
                     /gene="yoaA"
                     /locus_tag="N7C74_001998"
     CDS             complement(20923..22833)
                     /gene="yoaA"
                     /locus_tag="N7C74_001998"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HGU2431871.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            22965..23309
                     /gene="yoaB"
                     /locus_tag="N7C74_001999"
     CDS             22965..23309
                     /gene="yoaB"
                     /locus_tag="N7C74_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGU2431872.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5847 a         6030 c         5733 g         6155 t
ORIGIN      
        1 tgtaaaactc atcccacaga tgaattacct gatggttgtt gtggctttgt ttttccttaa
       61 cgccgtcatt tttcttttca tgttaatgaa atatttcact aacaaacaaa ttttaccaac
      121 actcatttta agccttgcat ttttaagtgg ccttatctat ttagttgaaa ccattgtaat
      181 tatccataaa ccaattaacg gcagtacact gatccagaca aagtcgaatg atgtttctat
      241 tttctatatt ttccgccaac tcagttttat ttgtttaacc tcgctggcgc tcttttgtta
      301 tggaaaagac aacatccttg acaacaataa gaaaaaaact ggcattctgt tgctggcgct
      361 gatccctttt ttagtttttc cccttctggc acacaatctg agcagttata acgctgacta
      421 ttctttgtat gtcgtcgatt actgtccgga caatcatact gcgacctggg gaatcaacta
      481 tacaaaaata ttggtttgtc tgtgggcatt tttactgttc tttattatca tgcgtacacg
      541 attagccagc gaactatggc cgttaatagc attattatgt ctggcatcgc tatgctgcaa
      601 cttacttcta ctgactctgg atgagtataa ttataccatc tggtatatca gtcgcgggat
      661 tgaagtctcc agtaaactgt ttgttgtgtc ttttctgatt tataacattt ttcaggagct
      721 gcaactctcc agcaaactgg cagttcatga tgtgctgacc aatatttata atcggcgcta
      781 ctttttcaac agcgtagagt cattattgtc gcgacctgtt gttaaggact tctgtgtcat
      841 gctggttgat attaatcagt tcaaacgcat caatgcccaa tggggacatc gtgtgggtga
      901 taaagtgctg gtttcaattg tcgatattat ccagcaaagc atccaccccg atgatatttt
      961 agcgcgactg gagggtgagg tgtttggctt gctatttacc gaactcaata gtgcccaggc
     1021 aaaaatcatt gcggaacgta tgcgtaaaaa tgtcgaactc ctgaccggct ttagtaacag
     1081 atatgatgtt cctgaacaaa tgaccatcag tattggcaca gttttttcaa cgggtgacac
     1141 gcgtaatatc tcgcttgtca tgacggaagc agataaagcc ttacgcgaag cgaaaagcga
     1201 ggggggcaac aaagtgatta ttcatcatat ttaagtgcaa aaatattcag agccatgctt
     1261 tttgcgtggc ttttgcatac aatttattac tattacccct aaatttcccc tccccagcgg
     1321 cgcagagatg agtataatta gcgcccctgt gccaggccgc aatcgaactt tatctggttt
     1381 tctcgtttca ctaaccgaag gagtgccatt tatcatgaaa ttgcaccata gaatgctccg
     1441 gcattttatc gccgcaagtg tcattgtgct gacatcttcc ttccttattt ttgaacttgt
     1501 cgccagcgac agagcaatga gtgcctatct gcgctatatc gtgcagaaag cagactcctc
     1561 ctttctttat gataagtatc agaatcagag tattgccgcg catgtgatgc gcgctctcgc
     1621 tgctgagcag tcggaagtgt cgccagaaca gcggcgcgcc atctgcgagg cttttgagtc
     1681 tgccaataat acccatggct taaacctgac tgcccataaa tacccgggct tacgcggcac
     1741 attacaaacc gcatccactg actgcgacac aattgtggaa gctgcagcac tattacccgc
     1801 ttttgatcag gcagtggaag gcaaccgcca ccaggatgat tacggttcag gtcttgggat
     1861 ggccgaagag aaatttcact attatctcga tctcaatgac cgctatgtct atttttatga
     1921 gccggttaat gttgaatact ttgcgatgaa taactggtcc atcctgcagt caggaagtat
     1981 tggcatcgat cgcaaagata ttgaaaaggt atttaccggg cgtaccgtat tgtcgagcat
     2041 ttaccaggat cagcgtacta aacagaacgt gatgagtttg ctgacgccgg tgtatgttgc
     2101 agggcagcta aaagggattg tgctgctgga tattaacaaa aacaatctgc ggaatatctt
     2161 ttacactcat gaccgccctc tcctctggcg ttttctcaat gtcacgctaa ccgatacaga
     2221 ttcggggcgc gacattatca tcaaccagag cgaagataat ctgttccagt atgtcagtta
     2281 cgtccatgac ttaccgggcg gcattcgtgt ctcgttatcc attgatattc tttactttat
     2341 cacgtcttcg tggaaaagcg ttctgttctg gattttgacg gcgttaattt tgctgaatat
     2401 ggtgcggatg cacttccgct tataccaaaa tgtgtcgcga gaaaatatta gtgatgcgat
     2461 gactggactg tataatcgca aaattttaac ccctgaactg gagcagcggt tgcagaaact
     2521 ggtgcaatcc ggttcttcgg tgatgtttat tgcaattgac atggacaagt taaagctaat
     2581 aaatgacacc ctcggtcatc aggaagggga tttagcgatt acgttgttag ctcaggcgat
     2641 taaacaatcg attcgtaaaa gtgattatgc catccgactc ggtggcgatg aattctgcat
     2701 cattcttgtc gattcgacgc cgcaaattgc agcacaactg cctgaacgta tcgaaaaacg
     2761 tctgcaacat atcgcgccgc agaaagagat cggcttctct tccggtattt acgcgatgaa
     2821 agaaaacgat acgttacatg atgcgtataa agcttccgat gagcgtttat atgtcaataa
     2881 gcagaacaaa aacagccgtt catgataacc ttctgtggtt gtttgcttgt aatctcagga
     2941 gcgtgaaatg actgaaatgg ctaaaggaag cgtgacgcat cagcggttaa tcgctttatt
     3001 atcacaagaa ggtgctgatt tccgcgtcgt tacccatgaa gcggtaggta aatgtgaagc
     3061 ggtgtcagaa attcgtggca ccgctctagg ccagggtgct aaggcactgg tatgtaaagt
     3121 caaaggcaat ggcgtaaatc aacatgttct ggcgatcctc gcagccgatc aacaggccga
     3181 tctgagccaa ctcgccagcc atataggcgg attacgcgct tcgttggcca gcccggcgga
     3241 agtcgatgaa ctcacaggct gtgtcttcgg cgcgatcccc cctttcagct tccatccaaa
     3301 actcaaactg gttgccgacc ctctactctt cgaacgattt gatgaaatcg ccttcaatgc
     3361 agggatgctg gataaatccg ttattttgaa aaccgcggat tatttgcgca ttgcgcaacc
     3421 agaactggtc aatttccgcc gcactgcgta actagcctgt ccgttcaata agcagaacgg
     3481 acaaaaccag cacaacagca atggcaaaaa acgatgacgt gataatcagt gtctcgacga
     3541 acatttgatc gttcatagca tacccccggg agatagctgt tttcacgtta ttgttagcgt
     3601 gcacaaatgg cagtttgatg acagttcgcc tgatttttta tgcaaaaaac gtaaagattt
     3661 tctacttcct tcctgcagca agcgtaaagt aagcaggctt attatttttt ggcaaggaaa
     3721 ccacgatgtt tgatgtcact ttgctgatcc tgctcgggtt agctgcgctg ggctttatca
     3781 gtcataacac cactgtcgcc gtttcaattc tggtgttgat cattgtccgc gtcacaccgt
     3841 taagcacctt tttcccgtgg attgaaaaac aggggctaag tatcgggata atcattctga
     3901 cgattggcgt tatggccccc atcgccagcg ggacgctacc gccctcaacc ttgattcact
     3961 cgtttctgaa ctggaaatcg ctggtggcaa ttgcagtagg cgttattgtc tcctggctgg
     4021 gtgggcgcgg cgtgacgtta atgggcagcc agccgcaact ggttgctggg ctgctggtgg
     4081 gcactgtttt aggcgtagcg ctgtttcgcg gcgtaccggt tggcccgctt attgccacgg
     4141 gtctggtttc gctgattgtg gggaaacagt agttaatccc gcaatatatc ggcctggcgt
     4201 ttgccccagg cctttcttaa acatagtaat aaaggccgtt gttgaatcat accccagcgt
     4261 atgagcaact ttctgtacag tatcgccttt cactaatccc tgaagcgcca taatcagctg
     4321 taactgctgt cgccactggc gaaaactcaa ccccgtctct tttactatca gtcgggcaag
     4381 attgcgttca ctcatcgcaa aaaagccagc ccattgcccc aatgcccccc actcgacggg
     4441 ctctttcgcc atcatctcca ccatcgcgcg gattttagga tgagaagaaa cgggtaggtg
     4501 cagctgttgt tgtggctgct gagggagttc atcaaacagg acctgaatta atctgcgggt
     4561 catcggctca gcacgctgag tcgtcgtcct gtttgccaga gttaaaatta attcccggca
     4621 taagggcgat attttcaatg tgtaacatgt tgtcggcatt gtcactgcgc tgggttcgat
     4681 aaacagaaaa cagagttcag cgttagctgt cacttgattg ctatgttcca ccccgcccgg
     4741 tatccaaacc gcatattgtg gcggcaccat ccacaaagca ttttccaccg tacaggtaat
     4801 tgcaccatgt agcgccagta tcagctgtcc tttgcggtgc tgatgtacag gactggttaa
     4861 ctcatccgtt ccggcgtgaa tacaaaatgc gaccgcggct tcatggtgtc ggtcgggttc
     4921 atagccattg agattcaacc tgtgcatcat tttgtccgaa tttagcgata atttgtcatt
     4981 ttagcttgat tcaacataac aataaaaacg gtaaggtaca gcctcgtttg taacaatgag
     5041 aagcatatga cctgttcaac ttcattaagc ggcaaaaaca ggattgtcct tatcgctggc
     5101 attctgatga ttgccactac attacgcgtc accttcaccg gcgcagcacc gttactcgat
     5161 acgattcgtt ccgcttactc gctgacgact gcgcaaaccg gcttattgac caccctgcca
     5221 ttattggcct ttgcgctaat ctcacctctg gctgccccgg tggctcgacg ttttggtatg
     5281 gaacgtagcc tgtttgccgc gttacttttg atctgtgcag gtatcgcaat tcgctctctc
     5341 ccttcgcctt acttattatt tggcggtaca gcggtcattg gcggtgggat tgcattaggt
     5401 aatgtcttac tgccaggatt aattaaacgc gatttccctc attccgtcgc cagacttacc
     5461 ggcgcatatt ccctgacaat gggagctgcg gcggctctgg gatcggctat ggtagtgccg
     5521 ctggctttga acggttttgg ctggcaaggc gcgttgctca tgctgatgtg ttttcctctg
     5581 ctggctcttt ttttatggct gccacagtgg cgaagccaac aacatgcaaa tttgagtact
     5641 tcacgcgcct tacatactcg gggtatctgg cgttcgccgc ttgcctggca ggtaacattg
     5701 tttcttggga tcaactcact ggtctattac gtgattattg gctggcttcc ggcgatcctc
     5761 atcagtcacg gctatagcga agcacaggcg ggttcactgc atggtttgct gcaactagcc
     5821 acagcagctc ccggtttgct gatcccactt ttcttacatc atgtgaaaga ccagcgtggt
     5881 attgcagcgt tcgttgcctt gatgtgcgca gtgggcgcgg ttgggctctg cttcatgcca
     5941 gcgcacgcga tcacctggac tctgcttttc ggttttggtt ccggcgcaac aatgatactg
     6001 gggttgacgt tcattggtct gcgggctagt tctgcgcatc aggcggcggc actctcgggg
     6061 atggcacaat ccgtcgggta tttgttggca gcctgtgggc cgccgctgat gggtaaaata
     6121 cacgatgcta acggtgactg gtccgtacca ctaatgggtg ttgcaatact ttcactactg
     6181 atggcgattt tcggactttg tgccgggaga gacaaagaaa ttcgctaata tccggtacta
     6241 tagtgacgtg acaaatcatg cgtgaaaggg agaacaaaca cgatgaatat tcagtgcaaa
     6301 cgcgtttatg atccggcaga acagagcgat ggttatcgcg tactggtcga ccgcctctgg
     6361 ccacgcggta tcaaaaaaac cgatttagcc cttgatgagt gggataaaga aatcacgccg
     6421 tcaacggaac tgcgcaaagc ctttcacggc gaagtcgtcg attttgcaac ctttcgcgag
     6481 caatatcttg cagaactggc acaacacgag caagaaggaa agcggctggc ggacatcgcc
     6541 aaaaaacagc cgctgaccct gctctactca gcaaaaaaca ccacgcagaa ccatgcgctg
     6601 gtgctggccg actggctacg tagcttgtga ttttagtaca gcatccggcg gttatttttc
     6661 accagccgga tggtccagtc gccacaatgc ccattcatca atcgtttcac cgcccggtaa
     6721 tttgcaattg ttgctgaccc cttgcgctgt ctgcactgga atgagcgtcc cgcccttctg
     6781 ctggcaatag accgacgccg gatttgccat accaatctgc ggcggtttag gtgcttctgg
     6841 ctgagaaggg gttgaacaac cagccaggac cagcaagcaa ggcagaacaa aactgataat
     6901 tttcatttat tgatctcaca tatgtatcca agcttagagt atcgcggtat cgttttgttt
     6961 tgcagcacta tttttattac attcactcaa aacatattac gtcttgtttc atctttgttg
     7021 atgatgtttt atcatgcctg caaagattaa ataatcagca tttacccgcc gtatcctgga
     7081 gttgtttcgt gtcagatcag attatcgccc gcatctcgca atcccttgcc aaagaacagt
     7141 cactggaaag tctggtccga cagcttctgg agatgctgga aatggtcact gatatggaat
     7201 caacctacct gaccaaagtg gatgtcgaag cgcgcctgca gcatattatg tttgcccgta
     7261 acagccagaa aatgcacatc ccggagaatt ttaccgtctc gtgggattac tcgttatgca
     7321 aacgcgccat tgatgaaaac tgctttttca gcgatgaagt ccctgaccgt tggggcgact
     7381 gtattgcggc acgcaatctt ggcatcacca catttctgag cacgccaatt cacttaccgg
     7441 atggatcatt ctatggcacg ctttgcgccg ccagcagtga gaagcgccag tggagtgaac
     7501 gcgcggaaca ggttttacag ttattcgccg gactgattgc acaatatatt caaaaagagg
     7561 cactggttga acagctgcgc gaagccaatg ctgcgctgat tgcgcaatcg tataccgact
     7621 cgttaaccgg gctaccgaat cggcgggcga tttttgaaaa tctgacgacg ctgttttccc
     7681 tcgcccggca tcttaaccaa aagataatga tcgcgtttat cgatctggat aacttcaaat
     7741 taatcaatga tcgttttggt cataatagtg gcgatctgtt tctcattcag gttggcgagc
     7801 gccttaatac gctccagcaa aatggcgaag ttattggtcg tctcggcggt gatgagtttt
     7861 tggttgtttc actaaacaac gagaatgcgg atatttcgtc gctgcgagaa cgcattcagc
     7921 agcaaatacg cggagaatat cacttaggtg atgttgattt gtattatcct ggtgccagtc
     7981 ttggcatagt agaagtcgat cctgaaacga ccgatgcaga cagtgcccta catgctgccg
     8041 atatcgcgat gtatcaggag aaaaaacaca aacagaaaac accttttgtc gcgcattcag
     8101 cgccacattc ctgaggcgta ttcacatcct tttgattggt gataacatgc gaatcggtat
     8161 tatttttccg gttgtaatct tcattacagc ggtcgtattt ttagcatggt tttttattgg
     8221 cggctatgct gccccgggag cataaagatg aaaaaaacaa cgatcattat gatgggtgtg
     8281 gcgattattg tcgtactcgg cactgagctg ggatggtggt aacgtcacat ctaaaaaata
     8341 gcaaaggctg cctgtgtgca gcctttgtgc aatttacgcg ttaactttta atcttcctgt
     8401 agataaatag cacgacaatc gcaccaataa cggcaaccac gaagctgcca aaattgaagc
     8461 catcgacttt accaaagcca aacagcgtgc tgatccaacc gccgactacg gcaccgacta
     8521 tccccagcag gatagtcata aagaatccac caccatcttt acctggcatg atccacttcg
     8581 ccagaatacc ggcaataagc ccaaaaataa tccatgacag aatgcccatt gtttcctcac
     8641 ttatctgttt tgcattagcg ggttagtcgc tgataaaaag catagcacaa catcgggagg
     8701 gcaagatttg tgacgagcat cacggaggtt tttttgcgat ggcgcagaaa ttgcgccatc
     8761 aacgatcagt gataattacc aaccacaaac atcatgttcg ttttccgtgt cataagaacg
     8821 cacggtattc accagatctt ttatcacttc agccgccact tctggcacca gcaaagtcat
     8881 cggcgtctct gtttcataat cgacagaaac gccattgctg ttattggtga cggtcacggt
     8941 atacgttgct ttgcccatga ttcatttccc gttatgaatg actttccgtt gttgcgcacc
     9001 ttccatcagg acttcaggag ccacgaagaa gtcaatgttg aaataagtat cgtcagtcat
     9061 ggcttcaatg ttgtgccact tttctggagg gaacaccgca aactgccccg cttcgataag
     9121 gatcacctga tcaggctctg cactgtgttc atcagcgtag ccgagatatt tgaccgcccc
     9181 atgcataacg gaaaggcgtg ggtaaacccc cgggcgcgtt cctttatcaa gatgacgttc
     9241 gaatattccg gcaggcgcag tttgtttatt ccagaaaggc gttgagcgcg tatgaatata
     9301 attctgtggg atttgcagca tccttttccc tccttcggtg aatgcgctga aaacggttta
     9361 ttccagccgt ttcagggtac gcctgataat ttgcttttta aataccattt attggttact
     9421 ttttagcacc atatcagaga aaaatcaggg aggattatag atgggaagcc cgcgacaacg
     9481 cgggctgaaa gcatcaggat tgcagcgtcg ccagtcgggc agcgaaaccc acgaacatca
     9541 aaccaatcag tgagttgccc actttagcca gtttcttttt ggtacgtatg tactgcgtga
     9601 caaaagcacc agatataatc aggaagctca aatagcagaa actcaccagt tccagcgtcg
     9661 tcgccagaat aaagaatgaa attcccgtat gtggggcatt aacatcgata aactgtacga
     9721 aaaacgacac atagaacaaa atggctttcg gattagtcag gctcaaaatt aacgcgcgtt
     9781 taaaaatagc accgtattgg ggctcatcgg atttggcctc gttattttta cccttcaggg
     9841 tcgcgtaaag aattttactc cccagataga gcaaataaaa cgcaccaaga taacgtacaa
     9901 tgttgaataa tatcggggtg gtcttaatta atgtcgccat tccagcccat gccagaaaca
     9961 tcaataccgc atcgccaata aatacaccgc aggccgcaag ataaccgcct ttcataccgc
    10021 tactgacgct atttttgagt acaaacaggg tatttggccc tggcaccaac acaataaaaa
    10081 tggccccaac cagataggtc cagtaattca gaaccccgta ttcagcgaac acattaacct
    10141 ctttaattat ctttcgatca tgcgcgatta aaggtgaata tgctaaccaa tctgtagcgg
    10201 cttagaaagg agaaaatcag gttttaacct gatatcaacc cgataattga atcattaatc
    10261 aggcatgctc cagtgaaaaa ttcgggtagt gctcggcaaa atactggcgt aaaaattcta
    10321 ccgttatccg cactttcgct gacgtcgcca gccttgaaac ataaacggcc cagacgttcg
    10381 ctggctggta atattccggt agcacttgca ctaaatgacc actggcaatg ttttcgctaa
    10441 catcccacca ggaacgcaga gcaatcccct gcccatcgag acaccactga tggacaattt
    10501 ctccatgatt agacgacagt gggccggtga ctttaatcgc atggtggcct tctttattgc
    10561 gtaattgcca gacaccaaaa ggatgatcgc gctctttaat gaccaggcac ggcaatgcag
    10621 aaagatcggt taaatgtttc ggcgcgccat gttgcgcaat aaattccggc gacgcgcaga
    10681 gaatgcggta attggtcgcc agtttgcggg cgattaaatt aggggcgatg tcatcgccaa
    10741 tgcgaatatc gagatcgaca ccttcattga ccaaatcgac cagccggtct tccacatcaa
    10801 aacgtaattc cagttgcgga tacgccttcg ccagcgctga taacgccgga gccaccacct
    10861 gtcgtccaaa accaaagctg ctgataatac gcagcatccc ctgtggcacc tgacgcacgt
    10921 cagaaagttc gtccatcatc tgaccgacat cctgcaaaat ccgctgcgcc cattcataaa
    10981 tccgctctcc ttcttcggta atggtgacgc ggcgggtggt gcggtgtaac aacaccacgt
    11041 ttagcgtttg ctccagcaag gcgatgcgct tgctgacgaa cgccggtgaa acgcccagtt
    11101 cttccgccac ggcggcaaaa ccagcccggc gagccaccag cataaagacg cgcaaatcat
    11161 tcagcagcgg taaattattc atgattcgtg ttttatgttt caccagttac ggggattaat
    11221 tccttttcag tcaattatag gatggtgatg ttgtcaattt tgatggtcag gaagtgagaa
    11281 cccaatgatg aaaacgatgc gtattgctgc gatcccggga gacgggattg gcaaagaagt
    11341 ccttcctgaa gggattcgcg tgttacaggc tgccgctgag cgctggggct tcgccttgag
    11401 ttttgagcaa atggagtggg cgagctgcga gtattacagc catcacggta aaatgatgcc
    11461 ggacgactgg catgagcaac ttagccgttt cgacgccatc tattttggtg ccgtcggctg
    11521 gccggacacc gttccggacc atatttcgtt gtggggttcg ctgctgaaat ttcgtcgtga
    11581 attcgaccag tacgtcaacc tgcgcccggt tcgtctcttt cctggcgttc cctgcccgct
    11641 ggcgggaaaa cagcctggcg acatcgattt ttacgtggtc agggaaaaca ccgaaggcga
    11701 atattcctcg ctcggcggta gagtgaatga aggtacagag catgaagtcg tcattcagga
    11761 atcggtattt acgcgtcgtg gtgtcgatcg cattttgcgt tatgccttcg aacttgcgca
    11821 aagccgccca cgtaagacgc taacttctgc cactaaatca aacggtttag ccatcagcat
    11881 gccgtactgg gatgagcgag tggaagcaat ggccgagaat tacccggaga tccgctggga
    11941 caagcagcat attgatattc tctgcgcgcg ttttgtgatg cagccggaac gcttcgatgt
    12001 ggtggtggcg tccaatttgt ttggcgatat cctttccgat cttggcccgg cctgcaccgg
    12061 caccattggc attgccccat ccgccaacct gaatccggaa cgcactttcc cctcgctctt
    12121 cgagcctgtc cacggttccg cgccggatat ctacgggaaa aatattgcta accctatcgc
    12181 cacaatttgg gccggggcaa tgatgctcga ttttctcggc aatggcgatg agcgtttcca
    12241 gcaagcgcat aacggtattc tggcagcgat tgaagaagtg attgctcacg ggccgaaaac
    12301 accggatatg aaaggcagtg ccaccacgcc acaggttgcc gacgcgattt gcaaaattat
    12361 tttgcgttaa ggtcaaacca gtttatttga accgcgtcac tgacgcggtt ttgttattcg
    12421 ttctttgcag ttaataacct gcgtcatttc accttttatt gtttccgttt cgtgttttat
    12481 ggctttccgt attcttaatt gtttaattta tgtaacatgc aaataatcaa ttcgttaaac
    12541 acgacataca actcattacc ctgcaaataa ttacaactgg caaaagccag agacgggctt
    12601 ttatgcccag gagatgactc atgatgagca atgttaagaa aaaagatgtg ccgctgataa
    12661 gcatcagcct ggtggccatt cttttcatcg cagctgcatt aagccttttc ccacaacaat
    12721 cggccgacgc ggccaacgca atatacactt ttgttacgcg tacgttaggt tccgcagtac
    12781 aggtattggt tttgctggca atgggactag tgatttattt agccaccagt aaatacggca
    12841 atattcgtct tggcgaagga aaaccggaat acagcacgct ctcctggctg tttatgttta
    12901 tttgtgccgg tttaggttct tctacgcttt attggggggg tgctgaatgg gcctattatt
    12961 atcaaacgcc tggattaaat atcgcaccgc gttcacaaca ggcactcgaa tttagcgtcc
    13021 cttactcctt cttccactgg ggcatcagcg cctgggcaac ttatacgctg gcctcattaa
    13081 tcatggctta tcactttcat gtgcggaaaa acaaaggtct gagtctttcc ggcattattg
    13141 ccgcgattac cggcgttcgc ccgcaaggcc catggggaaa actggttgat ttgatgttcc
    13201 tgatcgccac tgtcggcgca ctgactattt cccttgttgt caccgcagca acctttactc
    13261 gtggactttc cgcactgacc ggtttacccg ataacttcac cgtgcaggca tttgtgatcc
    13321 tgctttccgg cggcattttt tgcctaagct catggattgg tatcaacaac ggtttgcaac
    13381 gtctgagcaa aatggttggc tggggcgcgt tcctgctgcc attactggtg ctgattgtcg
    13441 gtccaaccga atttattacc aacagcatca tcaatgccat cggcctgacc acgcaaaact
    13501 tcctgcaaat gagcttattc accgatccgc ttggcgatgg ttcatttacc cgcaactgga
    13561 ccgttttcta ctggctgtgg tggatctcat acacccctgg cgtagcaatg tttgtcaccc
    13621 gcgtttcccg cggtcgtaag attaaagaag ttatctgggg actgatcctc ggcagcaccg
    13681 tcggttgctg gttcttcttc ggcgtaatgg aaagctatgc cattcatcag tttatcaatg
    13741 gcgtaatcaa cgtcccacag gtgctggaaa ctctgggcgg cgaaacagct gtacagcaag
    13801 ttctgatgtc gttgccagcc ggtaaattgt tcctcgccgc atacctgggc gtgatgatta
    13861 ttttccttgc ctcgcatatg gatgcagtgg cctacaccat ggcggcgacc agtacgcgta
    13921 atctccagga aggtgacgat cctgaccgtg ggctgcgtct tttctggtgc gtggtgatca
    13981 ctctgatccc gctttccatc ttgtttaccg gtgcttcgct ggaaacgatg aaaaccaccg
    14041 tcgtgctcac agcccttccc ttcctcgtca ttttactggt gaaagtcggc ggatttattc
    14101 gctggctgaa acaggattac gccgacattc cggctcatca agttgaacat tatctcccgc
    14161 agacaccggt tgaagccctg gaaaaaacac cagtgctccc tgcgggaacc gtattcaaag
    14221 gcgacaactg agcgccatca tcctaacgat aaaggtatcc ctatgagcaa tctgagccct
    14281 gactttgtac tacccgaaaa tttttgcgct aacccgcaag aggcgtggac cattcctgcc
    14341 cgtttttata ccgatcagaa cgcctttgaa cacgaaaaag agaacgtctt cgccaaaagc
    14401 tggatttgcg tcgctcacag cagcgaactg gcgaatgcca atgattatgt gacgcgtgag
    14461 atcattggcg aaagcatcgt gctggtacgc ggtcgtgata aggttttgcg cgcgttctat
    14521 aacgtgtgtc cgcaccgtgg tcatcagttg ttgagcggtg aaggaaaagc aaaaaatgtg
    14581 attacctgcc catatcacgc atgggcattc aaactcgatg gcaatctggc ccatgcacgt
    14641 aactgcgaaa acgtcgccaa tttcgatagc gacaaagcgc aactggttcc ggtgcgtctg
    14701 gaagaatatg ccggattcgt cttcatcaac atggacccca acgccaccag cgtagaagat
    14761 cagttacccg gtctgggcgc gaaagtgctg gaagcctgcc cggaagtcca cgatctgaaa
    14821 ctggcggccc gctttaccac ccgcacgcct gccaactgga agaacattgt cgataactat
    14881 ctcgagtgct atcactgtgg tccggcgcat ccaggtttct ccgactccgt acaggttgat
    14941 cgttactggc acaccatgca cggtaactgg acgctgcaat acggtttcgc caaaccgtcc
    15001 gaacagtcgt ttaaatttga agagggtacg gatgcggcat tccacggttt ctggctgtgg
    15061 ccgtgcacga tgctgaatgt caccccgatc aaagggatga tgacggtcat ttatgaattc
    15121 ccggtggatt ctgaaactac cctgcaaaac tacgatattt acttcaccaa tgaagagtta
    15181 accgacgagc aaaaatcgct gattgagtgg tatcgcgatg tgttccgtcc ggaagattta
    15241 cgtctggttg aaagcgtaca gaaagggctg aaatcgcgtg gctatcgtgg tcaggggcgc
    15301 atcatggccg acagtagcgg tagcggcatt tccgaacatg gtatcgccca tttccataat
    15361 ctgctggcgc aggtatttaa ggactaatga catcggcggc ggtattttcc gccgccgggc
    15421 tgatttttga tggagtacag caatgtcaga ctatcaaatg tttgaagtac aggtgagtca
    15481 ggtggaaccg cttaccgaac aggtgaaacg cttcacgccg gtggcaaccg atggcaaacc
    15541 gctgcctgcg ttcaccggag gaagtcacat catagtgcag atgagcgatg gtgataacca
    15601 gtacagtaat gcgtattcac tactgagttc accgcataac acctctagtt atcagattgc
    15661 cgttcgactg gaagaaaact cgcgcggcgg ttcccgcttt ttgcatcagc aggtaaaagt
    15721 gggcgatcgg ttaacgatct caacgcctaa taacctgttt gcgctaattc cctcagccag
    15781 aaagcatctg tttatcgcgg gcggtattgg tatcacccct ttcctgtcgc acatggcaga
    15841 gttgcaacac agcgacgtcg actggcagct acattactgc tcgcgaaatc cagaaagttg
    15901 cgcatttcgt gatgagctag tccagcatcc gcaggctgag aaagtccatt tgcatcattc
    15961 atcaaccgga acacgactgg aattagcgcg attattggcg gatatcgaac ctggcacaca
    16021 cgtttatacc tgtggccccg aggcgctaat tgaagcggta agaagtgaag ctgcgcgtct
    16081 ggacatcgct gccgatacgc tgcactttga gcaatttgct atcgaagaca aaaccggcga
    16141 tgcatttacc ctggtgcttg cccgttccgg aaaagagttt gtggtgccgg aagagatgac
    16201 tattttgcag gttattgaaa ataataaagc cgcgaaagtg gaatgtttat gtcgtgaagg
    16261 ggtatgcgga acctgcgaaa cggcaatact ggaaggtgaa gctgaccatc gggatcagta
    16321 ttttagcgat gaagagcgtg ccagccagca aagtatgttg atctgttgtt cgcgtgcgaa
    16381 gggtaaacgc ctggtgttgg atttgtagtt tgccgaagcc ggatgtggcg ctgagcgcgc
    16441 ccagtccggc ttcggaaaat tttactgcgg atattcctgc aataaattgt gtaatgcttc
    16501 cgccatcagc tcaccacgcc agccggaaat cagctccggc aaattgttct gcggtttcag
    16561 tttccagtgc cagttcagca gttggttgat ttgccgacgc gatgccagca attcggcgct
    16621 gatcttgtgc gtttcgctca cgtcagtaat cagcgactta atcgctttaa acgctttacg
    16681 ataacccggc atgtccatca ggttaagcat cggctgcggt aaggcctctt ccggcaatgt
    16741 ctgcgctttt tccaccagcg ccagcagcgt tttaccgtga aagcggattt cgctaccgga
    16801 taaacccagg ctgtccagtt cgcctaaact tcccggcata taacgcgcta ccgaccacaa
    16861 atgctcttca cgcacgacaa agttcaccgc cagatcgcgc tctcgcgcct tgcgcagtcg
    16921 ccagtcggct aacagttgca gacaggccag ttggcgtgtg cgtaattgcc aggcattggt
    16981 gatatcacgc caggcatctt ccggcgcaac gacttcctga cgacgcattt gcatcaggcg
    17041 acattcatcc agcgccgctg gtagccagcc ggaggcctcc gtttctacca taagcttggc
    17101 ggtgatcggt aacagatacc agacatccgc cgctgcatat tcacactgac gttcggtcag
    17161 cggtctggcc agccagtcgg tgcgcgattc actcttgtcc agcgtaacgc cggaatactc
    17221 ttccaccatg gaagcgaaac cccatgacat cgggcgtccg cagaaggcag caaggatttg
    17281 cgtgtcaatc aggggttgtg gtaattcgcc aaagacattg aggaacactt ccagatcttc
    17341 actgcctgca tggagaaatt ttgtgatgga cggatcgcgc aggatcgctt tcagcggtga
    17401 ccagtcggtg atcccgagag gatcaattag cgccagatgc tcgccatcga aaagttgaat
    17461 caaccccagc tgcgggtaat aagtgcgcgt acgaacaaat tcagtatcca gggctatcgc
    17521 cggaaaggca cggacggctt cacacaaaga agccagcgca tcgtccgtgg taatcatttg
    17581 gtaattcaaa ttgttttctc tttagtgggc gtcaaaaaaa acgccggatt aaccggcgtc
    17641 tgacgactga cttaacgctc aggctttatt gtccactttg ccgcgcgctt cgtcacgtaa
    17701 ttctcgtcgc aaaatttttc cgacgttaga tttcggtaac tcatcacgaa actccaccag
    17761 cttcggtact ttgtagcccg tgagctgacg gcggcaaaag gtcaccagtg actcttcggt
    17821 aagcgatgga tcttttttca ctacgaagat tttcaccgct tcaccactgg agccggaagg
    17881 tacaccaaca gccgcgactt cctgtacgcc aggatgctgc atgacgacat cttcaatctc
    17941 gttgggatag acgttaaaac cggaaaccaa aatcatgtct tttttacgat cgacaatgcg
    18001 caggaatcct tcttcatcca ttaccgcgat gtcgccagtg tgtaaccagc cgtttttgat
    18061 gatttcatcg gtagcatccg gacgctgcca atagcccagc atcacctgtg gacctctgac
    18121 gcaaagttca cctggctgac ctggcgatac ttcattatcg tcgtcatcca ccagtttggc
    18181 ttccgtcgac ggcaccggca aaccaatgct accgctatga taatcaatat cataggggtt
    18241 cacgctgacc agcggcgcac attcggtcag gccataacct tccagcagat actgtccggt
    18301 cagtttcacc cagcgttctg ccaccacttg ctgaacaggc atcccaccgc ctgcagaaag
    18361 gtgcagactg gagaaatcca gttgttgaaa ctctttattg ttcagcaacg cgttgaacaa
    18421 ggtgtttacg ccggtgatag cggtaaacgg atatttcgcc agctctttga ccagccccgg
    18481 aatatcccgc gggttagtga taagcaggtt ctgcccaccc agttcgataa acagcaggca
    18541 gttaatggtc agtgcaaaaa tgtgatacag cggcagcgcc gtcaccacca gctctttgcc
    18601 cggatgcagc agaggcccat acgtagcgtt aacttgttcc aggttcgcca gcatattgcg
    18661 gtgagtcagc atcgcgcctt tcgccacacc tgtggtgccg ccagtgtatt gcaggaaggc
    18721 taaatcttct ggcaccagtt caggtttgac atactgcata cggtagccgt tgtgcagggc
    18781 gctacgaaat gaaatggcat ctggcagatg atatttcggc accagacgtt tgatgtattt
    18841 aacaacgaag ttaaccagtg tgcctttcgc tgtggaaagc tgatcgccca tacgggtcag
    18901 gataacgtgc tgaactgcgg ttttatcgac cactttttcc agcgtgtgag caaagttgga
    18961 cacaatgaca atcgccgatg cgccgctatc gttaagctga tgctcaagct cacgcggggt
    19021 atacaacggg ttaacgttta cgacgatcat cccggcacgc aaaatgccaa acagcgccac
    19081 cggatattgc aataaattag gcatcatcaa cgcaacgcga tcgcctttct tcagccccaa
    19141 cccttgttgc aaataagcgg caaacgcgcg actgcgttct tccagcttgc ggaaggtcat
    19201 cacctccccc atattcacaa acgcaggctg atcggcgtag cgcgcgaccg actgctcaaa
    19261 catatctacc agagattgat aacggtcagg gttgatctcc gtcggaacgt ccgcgggata
    19321 acggttaagc caaaccttct tcaattcttc acctctaaaa tgcgtgttcg tcgtcatcgc
    19381 aaccccaaat gatatacatg ccgttaacat aatattaact catcatacca gcttgataat
    19441 tacccaacga aaaggttgcg aagcgcgtca ctatttattt ttatctttac cgtaagaatg
    19501 cagaaacagc ggaccagccg ctgtttcttt ttctttaaaa acaagcaatt attcagttac
    19561 aactgtttgt actctcgcgg ggccgggatt ataccagccc catccgccgt ggccataggg
    19621 ccagccacga ccgccataaa accagggatc aatcggctga ggcggcataa tcacctgctg
    19681 ggttaaatgc caacgtttgt aacctgttgc ctgcatcacc ataaatttat agggcgtgtt
    19741 gccgattttt ccgtcaaccg caccagtgat tggcccgact accgtaacca gttgtccacg
    19801 gaaatccacc gggtccagaa aaccgttcac atcggcataa atgcgaccgc gagaaggttc
    19861 tcccagcgtc ggtctggctc cgctgtccag cggtacggta gcaatttcca ggcgggtttt
    19921 cccttgctgg ttttgtaccg caaccacttt gccaccaaag cgtgcctcct gaccaacgta
    19981 cagctgcggc gcactcatca cccgaactaa atcttgttgc ggcgtgggac tgctgccttt
    20041 aatggcgtcc ggcacagtga cacaaccgct cagcattagc gcaaacgtac ctgccagtat
    20101 gcctttgata acattctttt gaaccgccat gatgcgactc ctttttttct cagggcatac
    20161 tcttaagatt cattctttgc ccggaagttt cttccatgcg acgttgttac gtaaataaac
    20221 cggttcggca tgttccaccg ccaccgtttt accctcggca aacatctgac aagcaatcgg
    20281 cagcatatct tctgcagcgg gcagtaaaac ttctccgtcg cgcaaaacca gtccgctctc
    20341 tttaccgaga tccggccagg cttgccagcc tgtacctacc gtcacccatt ccccggaaag
    20401 ctgttgcatt cgttcatgaa cgagttcggg tttgagtacg gcttcggttt cttcaccgtg
    20461 ccagataccg ttttcatcac gctgatattc ggcccagtaa acttcgccca ttcgcgcgtc
    20521 aatggctgac agcacgcgtg ttgcgccgtt tttgcgccac gccccttgcg ccatcgtcat
    20581 tagcgtggag acgccaatca tcggtaattc cgcgccaagc gccagccctt gcgcgatgcc
    20641 aataccaatg cgcacgcctg taaagctacc ggggccgcgc ccgtaagcca gagcgttaat
    20701 atcagtcagg gaagttccgc tggtggtcag gatatcctgc accatcggta agattcgttg
    20761 agtatgttca cgagggcaaa gctcaaaatg agcgttgaca gtaccgtcgt tccacagggc
    20821 gacagagcag gcctctgtcg cggtatcgat agccagaatt cgcatgggtc ttcgtgctta
    20881 gatcaataaa aaggcgcgca tcataccata ctccgtaaca aattacctgg aggatggtat
    20941 cgcaaggaaa cgaaccgcac gggcaatgtc acgggtgcgt ggcgcgggcg gcagactggc
    21001 gagaaacgtc gcgccgtaag gacgcatcac cagccgattg tcacaaatta ccaaaacccc
    21061 gcgatcgtcg gcgtcgcgaa tcagtcgccc gactccctgc ttgagagtaa tcaccgcatc
    21121 cggcagttgt acttcatcga acgggtcgcc accgcgcaaa cggcaatctt ccatgcgcgc
    21181 ttttaacaat ggatcatcag gcgaggtaaa cggcaatttg tcgataatca ccaatgacaa
    21241 tgtatcgcca cgcacgtcca ccccctccca gaaactactg gttgccacaa gaagcgcgtt
    21301 accggcactg acaaattgct gcaacagttg ccctttgctg gtttccccct gcaataatac
    21361 gggaagcgtc atggtagcgc ggaactgctc ggccagatcg cgcatcatgg cgtgcgaggt
    21421 acaaagcata aaacagcgac cgttgttagc ttcgatgatc gggcgcagca tagccgccag
    21481 ttgccgcgcg gaacccggtt gattggtttg cggcaggttg cgcggcacac agagtaacgc
    21541 ctggcggctg taatcaaacg ggcttggcaa tagcaacgac tccgcctgtt ctatcccaag
    21601 ccgcgaggtg aaatgatgca gatcgtcgtt taccgacagc gttgctgagg tgaagatcca
    21661 gctaccgggc ttttgcgcca ttaactcttt gaatttatcc gccacgctga gcggcgtgag
    21721 agccagagta aaatggcgcg aagtgcattc gtaccagtag ctgtagcccg gctgattgat
    21781 ctcttttagc cgcttcagcc gtgtgcgata caacgtggcg cgctcaaatg ccgcatccag
    21841 caaggcggaa cgccccagtg acagtttcgc cacgtcataa caaagttcca gggtgtcatc
    21901 gagcagtaaa aatgcccgct gaatttgcgg attagctaac agctcacgca ggttaccgcg
    21961 ataacctggt tcaccgagtt gcagacgaaa atcctgcgca ctctgggcaa gacgatcagc
    22021 gcacttttgt aactgctggg tgtcttttaa ttcggtgcgg taggcgatgg tgatgtcttt
    22081 tgccaggtcg agcagttgtc gactggagag tgactgacca aaatactggc tggcaatgtc
    22141 cggtagctgg tgggcttcgt cgaagatcat gacgtccgct tccgggatca actcgccaaa
    22201 tccactctct ttaaccacca tatccgccag aaagagatga tggtttacca ccaccacatc
    22261 ggcgtccatc gcttttttac gtgctttgac cacaaagcaa tctttataca tcgggcagtc
    22321 gctgccaaga cagttgtcgt tggtgctggt gaccagcggc cacgcctgcg aatcttccgc
    22381 cacgctgacg caggtgctga tatcaccatc gactgtttga ttagaccagg agcgcagcag
    22441 gatcacatcg cttaatattt gtaccggaag atcgcccccc gccagcgcct gctgttcgag
    22501 acgttcgagg cagaggtagt ttgagcgccc tttcagcaac gccacgttgc ccgtgtattt
    22561 caacgccttt gagaccgtcg gcaaatcgcg gctatagagc tgatcctgca acgcttttga
    22621 gccggtcgag ataatgactt tctttttcgc ccgcagcgca ggggccaggt aagcgtaggt
    22681 tttgcccgta ccggttcctg cttccaccac cagcggctgg cctttttcta tcgcctgggt
    22741 gacggctacc gccatctgtc gctgtggttc tcgcggctta aagcctggta tcgctttcgc
    22801 cagctgaccg tctggtgcaa aatcgtccgt cacactaccc cctgttgatt tgaacaggga
    22861 ttatgtcagg atgagggcgc tttcgccagt tgaagtggtg acggcgacct cacattgtgg
    22921 cagtctttgc agcacgaaaa tggaaggata atgaggataa aatgatgact atcgttcgta
    22981 tcgatgctga agcccgctgg tctgatgtag taatccacaa caacacgctc tactacactg
    23041 gtgtaccgga aaacctcgac gccgatgcct ttgagcaaac cgccaacacc ctggcgcaga
    23101 ttgacgccgt gctggaaaaa cagggcagca ataaatcgag cattctggat gccaccattt
    23161 tcctggccga taaaaacgac ttcgcggcga tgaataaagc gtgggatgcc tgggttgtcg
    23221 cgggtcatgc gccggtgcgc tgcacggtac aagcgggttt gatgaacccg aagtataaag
    23281 ttgaaattaa gattgtggct gcggtgtaag ctttatcgaa gcaaaataag tcagacgata
    23341 atttatcgat aatactggtc ggttatacat aaatcgacca gagaataaga tattactgtt
    23401 gatgcttctg ctaattcatt atttattttt ataatttcaa ttttatctat aaaactaact
    23461 atataaataa acataaccat gccgttgtgt tcgccctttg attagcaact ctggttttct
    23521 ttcttagctg aacaatctgg ttatagcgtg cggactgacc gttaattttc tccatcttac
    23581 tggataacac accatgagct gctaccttta aaacacctga atatcaacag gttaactcgt
    23641 gccatatcgt tataatccct ttgatattag atgcaaatta aggtcatata tagccttatc
    23701 gataacatgg ttaattttaa ggataagaat atgcctgctg taatagataa agccctggat
    23761 ttcat
//
LOCUS       DBFIFM010000059        23745 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.059, whole genome shotgun sequence.
ACCESSION   DBFIFM010000059 DBFIFM010000000
VERSION     DBFIFM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.059"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..3859)
                     /gene="entF"
                     /locus_tag="N7C74_002000"
     CDS             complement(<1..3859)
                     /gene="entF"
                     /locus_tag="N7C74_002000"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HGU2431873.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSELPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEQTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAAFWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQHYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLREHGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFVPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMAPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPLWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPI"
     gene            complement(3856..4074)
                     /locus_tag="N7C74_002001"
     CDS             complement(3856..4074)
                     /locus_tag="N7C74_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HGU2431874.1"
                     /translation="MAFSNPFDDPQGVFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(4077..5279)
                     /gene="fes"
                     /locus_tag="N7C74_002002"
     CDS             complement(4077..5279)
                     /gene="fes"
                     /locus_tag="N7C74_002002"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HGU2431875.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLISL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5522..7762
                     /gene="fepA"
                     /locus_tag="N7C74_002003"
     CDS             5522..7762
                     /gene="fepA"
                     /locus_tag="N7C74_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HGU2431876.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDNDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGITWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            7937..8557
                     /gene="entD"
                     /locus_tag="N7C74_002004"
     CDS             7937..8557
                     /gene="entD"
                     /locus_tag="N7C74_002004"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HGU2431877.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8679..8831)
                     /gene="hokE"
                     /locus_tag="N7C74_002005"
     CDS             complement(8679..8831)
                     /gene="hokE"
                     /locus_tag="N7C74_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HGU2431878.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(9182..9334)
                     /gene="hokE"
                     /locus_tag="N7C74_002006"
     CDS             complement(9182..9334)
                     /gene="hokE"
                     /locus_tag="N7C74_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HGU2431879.1"
                     /translation="MLTKYALVAVIVLCLTVPGFTLLVGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            9303..9570
                     /locus_tag="N7C74_002007"
                     /pseudo
     CDS             9303..9570
                     /locus_tag="N7C74_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9773..10891
                     /locus_tag="N7C74_002008"
     CDS             9773..10891
                     /locus_tag="N7C74_002008"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HGU2431880.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHKEKD
                     YLLYKFNRFQACRYGLEGVITDPYTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            10957..11205
                     /locus_tag="N7C74_002009"
     CDS             10957..11205
                     /locus_tag="N7C74_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HGU2431881.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            11270..11638
                     /locus_tag="N7C74_002010"
     CDS             11270..11638
                     /locus_tag="N7C74_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGU2431882.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            11732..12385
                     /gene="nfsB"
                     /locus_tag="N7C74_002011"
     CDS             11732..12385
                     /gene="nfsB"
                     /locus_tag="N7C74_002011"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HGU2431883.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            12493..13740
                     /gene="ybdG"
                     /locus_tag="N7C74_002012"
     CDS             12493..13740
                     /gene="ybdG"
                     /locus_tag="N7C74_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HGU2431884.1"
                     /translation="MQDLISHVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(13808..15184)
                     /gene="pheP"
                     /locus_tag="N7C74_002013"
     CDS             complement(13808..15184)
                     /gene="pheP"
                     /locus_tag="N7C74_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HGU2431885.1"
                     /translation="MKNASNVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLAMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(15286..18423)
                     /gene="cusA"
                     /locus_tag="N7C74_002014"
     CDS             complement(15286..18423)
                     /gene="cusA"
                     /locus_tag="N7C74_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HGU2431886.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKIQVGPEMRRGIA
                     ELNGEVVGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRAID
                     NLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMSLG
                     GIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITNASVEVGPALFI
                     SLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGKIP
                     PESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQINEG
                     DLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMVET
                     TIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIGIK
                     VSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTVAD
                     VQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITLAD
                     VADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSVAF
                     SGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWLLW
                     WMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEALH
                     HGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFIIP
                     AAYKLMWLHRHRVRK"
     gene            complement(18435..19658)
                     /gene="cusB"
                     /locus_tag="N7C74_002015"
     CDS             complement(18435..19658)
                     /gene="cusB"
                     /locus_tag="N7C74_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HGU2431887.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(19674..20006)
                     /gene="cusF"
                     /locus_tag="N7C74_002016"
     CDS             complement(19674..20006)
                     /gene="cusF"
                     /locus_tag="N7C74_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HGU2431888.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGFDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSGIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(20030..21412)
                     /gene="cusC"
                     /locus_tag="N7C74_002017"
     CDS             complement(20030..21412)
                     /gene="cusC"
                     /locus_tag="N7C74_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HGU2431889.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALENNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTVLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            21569..22252
                     /gene="cusR"
                     /locus_tag="N7C74_002018"
     CDS             21569..22252
                     /gene="cusR"
                     /locus_tag="N7C74_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HGU2431890.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            22242..23690
                     /gene="cusS"
                     /locus_tag="N7C74_002019"
     CDS             22242..23690
                     /gene="cusS"
                     /locus_tag="N7C74_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HGU2431891.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVFLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELQFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHLVQVIVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSNARGTRFVIVLPERG"
BASE COUNT         5697 a         6492 c         6040 g         5516 t
ORIGIN      
        1 taatcggccc aattttttca aatgcccctg gagagataat atccacatgc gcacaatcct
       61 gacgatagat atccagctcc gcaatccacg gcgaccaggc gcgttcgggg ctcatacctt
      121 cctgaagcgt acgttcagca acaaacagcg tcgcttttcc gtcaaacggt acgctatgag
      181 cagtcgtcag caggcgcaca gcatcagcgt agttgccttc aatggtggta aacaactccg
      241 ttgaagtact tccctgctgt gccgccagga aggcctcgcg ctcgcggtta atctccgcca
      301 gcacttccgg gtccagacca ttagcttctt tttcctgcca gttttgcgtt tctggcggcc
      361 aggtatccag caagccaaga aatgccacct gttcgccacg ggcacgtagt cgcgccgcaa
      421 tgccctgcgc cagtgtaccg ccaagggaat accccagcag gtaataaggg ccgtgcggtt
      481 gttgttcaag taacgttgcc agatgcgctt cgcagacttc atccaggttc gtcgctgtct
      541 gcatggggcc atgagggcgc ggcgactgaa tgccgataat cgaccatagt ggatcgagat
      601 aacgcgagag cacgctaaac tgccaggcaa aaccggatgc cggatggaaa caaaacagcg
      661 tcgggccatt accttcacgc aacggcagaa tggtttcgaa tcccatgcgc cgggagctgt
      721 cctcttcacc atcaataatc gttgccagtt tggcgacggt tgacgcgacc atcacctgcc
      781 ccggcgtcac ctgacgggca aactgccgac ttaactgcgc tgccagtttc attgccagta
      841 gcgaatgacc gccaagcgcg aagaaatcag catcggcatc ctgcacgtca caacccagca
      901 acgacgcgaa tgccgcagcg ataatcgttt cactgcccgc tttcggcgca cgccccggcg
      961 tttgtgcctt aagttcaggc aacggtaagg ctttgcgatc cagcttgccg ttggcgctaa
     1021 gaggtaactg tggaagttgc agcagaacga ccggcgccat atgcggcggc aatgtttcgc
     1081 gaagctgtgc ctgtaatgcg ctggtatcca acggcagacc tgattgcgac accagatagc
     1141 ccaccaactg acgcgcatca ccaccggtgg ctgccgcctg gttaatcaca caggcgtggg
     1201 taacggcttg ttcgacatcc ggcagcgcct gcatcacgcg atcgatttcg cccagttcga
     1261 tacgctgccc gcgaattttt agctgatcgt cactgcgccc gaggtactcc accgcgccgt
     1321 tatccagcca gcgggcaacg tctccggtac ggtacatccg ttcaccaggg acaaaaggat
     1381 cggcaatgaa gcggctggcg gtcagatcgg gtcgtccaag atacccctgc gccagttgaa
     1441 taccggtgag atagagatct cccgccacac ccggcggcac cggatgcatc atcgcatcga
     1501 gaatgcgcaa gcccgtattc cacaccggat aaccaatcgg cacactgctg ccgcgcacct
     1561 gtgccagttc ctcgccaaaa gccggatacc aactcacatc taccgccgct tccgtcgggc
     1621 catatagatt atgcaacggc gcgcccgtta actgttgcca ttcgcggcat aaatcagccg
     1681 gtaaggcctc accactacag aaaacctgtt tcaacgtcgc gcaattctgg cgagcggttt
     1741 gcggcgtcag cgatgcaaca aatgccgcca gcatcgacgg cacaaagtgc gtggtcgtta
     1801 cgccatattc ggcaaagaat tgctgcatag cgagcgggtc gcggtgcgct tccggttcag
     1861 ccatcaccag tttagccccg gcaataaacg gccagaaaaa ctcccacacc gagacatcaa
     1921 aactgcacgg cgttttttgg gcaacgacat cttcacctgt aagtggataa tgattttgca
     1981 tccacaacag gcggttaacg atagccgtct gcccgaccat tacccctttc ggcctgccgg
     2041 tggagccaga ggtaaagatg atataagccg tgtgatgcgg ttgtgaaagt tgcagcggcg
     2101 cactgccctg cggtgtaagc ggggcgttat agcaaaggtt ggttaaatct ggaacatcgg
     2161 caaagcgcgg cagttgatcg tcggtggtga ttaacagcga cggacgcgca tcttccagca
     2221 tcattttcag gcgatcgtcc ggataaccgg tatccagcgg taaccaggcc gcacctgctt
     2281 caacaatcgc atgtagcgcc agggtcaaaa agaccgagcg cggtaatgcc accgccacgc
     2341 tgtcccctgg tttaacgccg tgctcacgca gcagattcgc cagcgccacc acctgctcgc
     2401 gcatttcccg atagctgaac tggtaacgcg catctgccag cgccggagca tccggtgttt
     2461 ttgccgcttg ttctgccacc agcgcgctaa gcgtggtttc tggaatctca acctgagtgg
     2521 cgttgatctg cgccagctgc gcatactcac ctggcagcat aatatcaaca tcgccgcaca
     2581 acagagccgg atccgcagcg aattgggcga tcagcatttt caggcgttca gcatgctgga
     2641 ttaacgttgg ctcatcgtaa tgctgtttat tggcgaggat ctcaatactc aaatcaccgt
     2701 gttcatccgg gaacagggcc agttcaaggt cattaaccgg accggttgcc agggtatggg
     2761 tttgcgcctg aacaccagga atatccagtt ggtaatcaaa taccttgata ttgagtaccg
     2821 gaccaaacag cggttcatca cctgccgctc gcccgctgtc acggacaatt tgttcggcat
     2881 cgtaacgttg atgacgacgc atttttttca gttgtgctgc cagtcgggtt gccagttccg
     2941 gcagcgtttc ttgtgccgca atgtgaatac ccaacggtaa aacgttgagc acgggtccgg
     3001 tagccgtcag cgccgccgag cccagtcgac gcataaagat aaatccggcg gcgtagtcca
     3061 tgcgattgca caatcgcccc agccaaaagg ctgccagcgc aagggctaaa tcggtacgct
     3121 gcacacctga aagttgcgta gccagctggc ggaattcccc gtcggtaaat tccagtttca
     3181 ggcgtagaat atctgccgaa gcgctgcgcc ccgctaaagg tgccggagaa agtgacgcgg
     3241 gcggcggcag ttgacgacgc tgttccgccc agaatgccgc atcacgctgc caggcttcgc
     3301 tttcgcggta ttgctggtac tcttccacta catcagcgaa aggcgtaaac ggcgaagcag
     3361 gcgtttgttc gccacgcagc aatgcgcagt aaatgttggc gatctgccga gtaatggccg
     3421 ggaaactgaa gccatcgacc agcaaatggt gataacgctg ataccagtac cagcggttat
     3481 ctgccacctg tatcagctgg tgaaagacca gtggtttacc gctatcgacg cgcaaatctt
     3541 gttgcaaatc cgcctgcatt aatgcctgcg cagtaccgtg cggatcaata ttggttcgca
     3601 ggtcgataat ttctggcagt tcgaatatca gcgcatcatc gacccattgc cagacttcgc
     3661 cgttatcttc cgtaaaacgc atccgcagcg tatctgcttg cgctaatccg gcaaccaccg
     3721 cgcgggccag taatggcgca tcaacctctc cggttaactc aacgtaatgc gccacgctcc
     3781 aggcggaggg taattctgac agtttttctg ccatccagat gccgggctgt gcggcgacca
     3841 aaggtaaatg ctggctcatt gtgcctcctg caactgggtg aaattcgccg gtgtcagagt
     3901 acgccagtgg acttccagcc actgctggca ggactcttgt gactgcggct gacacacaat
     3961 gtcccagcct gccggtaagg cgcattgttg cggccacaga ctgaattgcc cctgcgcatt
     4021 gcgcaaaatg taaaacactc cctgtggatc atcgaaggga ttactgaatg ccatattcaa
     4081 ctcctgtcat ggaaaagtgg ttgccagagg tcgattaacc cctgcatcaa gccaccgcgc
     4141 caacaaagcg catcatgtcc gccgtcaacc tgacgccaga aaatggattc ttttataggg
     4201 tgtaattgcg catacagcgc ctgattggcc cgcatgatca tcggctcgcg aatacccgct
     4261 tccagcacaa tgcgcagacc ttcggcgcta acttcaccag cttttagctt ttcaagtaac
     4321 acgccctctt gctgcccgcc ccgatgcggc caccagtacg atcctgactg gcttaataca
     4381 cagccaaagc gttcaggcca gtgcagtccg gcatacagcg cggacagccc accaaaactc
     4441 tgcccggcga ccacggtgcg atcggcgcga tcgctaaaag gggcaatagc tttcaccagg
     4501 ggtaataact cttgctgtac tgccagccag aaatccgcat tacacggcag ttcgtgggcg
     4561 cggtgcgtgg tgtcgatagc gtcgatcaac acatacacgg cgggaggaag ttgctgacga
     4621 tgggtcagcg aaatcagcac aggccagacg ggcatacttt gcgcccaaaa ttcgccatcg
     4681 agcaaaactg ccagcgggcg ttcttctgct gttacatcgc cggtggtaaa aatccataca
     4741 cgacgtgaat ttttcaaccg ttcacttttc cagataattt ctttggctgg tatttctggc
     4801 gcttgcggac aatcccatcc cggttgcaga ggcgcttgcg gcatttcgag tgcagaaaca
     4861 gcgtgccctc gcccgccttt ccagctttgt ggattcagcg gatcggctat tgcctggggt
     4921 aatagttttc gccagccttc acgtaattcg aggcgatcgg gggatggtgc agaaaaaatg
     4981 tcatcgcgtt cggtgggaat aaagcagtag ctgccgcgcc agttggcatt gagttgtgtc
     5041 gtccactgcc agacatcagt acctgcaatt cgctgcatcg actggggctg gctgttctga
     5101 tgatgatcgg tcacaccagt gatgtagatc catacgcgct ttatcgtcga gtattcttca
     5161 gaaccttggg gatcacgcca ccagaaagtg acctcaaaca tttcgtcatt cagacgctgc
     5221 cattccgggc catgtttcga ctgccaccag ctctcacttc ctacttttaa cgccgtcaca
     5281 ccataacccc atgtttactg tgcaattttt cattgattgc agaaatatat tgataatatt
     5341 attgataact atttgcattt gcaatagcgt aatggcgcgc cgtgggaagc gcggacatta
     5401 attaaccaac tgcactgcgt gtctttcagg atcaaaggtt ttcgcggtag cgggatgcgt
     5461 cgtgttgatg acgaccatgc ccgacagttg caattcgtgg caaaaatgca ggaataaaac
     5521 aatgaacaag aagattcatt ccctggcctt gttggtcaat ctggggattt atggggtagc
     5581 gcaggcacaa gagccgaccg atactcctgt ttcacatgac gatactattg tcgttaccgc
     5641 cgccgagcag aacttacagg cgcctggcgt ttcgaccatc accgcagatg aaatccgcaa
     5701 aaaccccgtt gcccgcgatg tttcggagat catccgtacc atgccaggcg ttaacctgac
     5761 cggtaactcc accagtggtc agcgagggaa taaccgacag attgatattc gcggtatggg
     5821 tccggaaaac acgctgattt tgattgacgg caagccggta agcagccgta actcggtgcg
     5881 tcagggctgg cgtggcgagc gcgatacccg tggtgatacc tcctgggtgc cacctgaaat
     5941 gattgaacgt attgaagttc tgcgtggtcc ggcagctgcg cgttatggca acggcgcggc
     6001 gggcggcgtg gttaacatca ttaccaaaaa aggcagcggc gagtggcacg gctcctggga
     6061 cgcatatttc aatgcgccag aacataaaga ggaaggtgcc accaaacgca ctaactttag
     6121 cctgaccggt ccgctgggcg acgaattcag cttccgcttg tatggcaacc tcgacaaaac
     6181 ccaggctgac gcgtgggata tcaaccaggg tcatcagtcc gcgcgtgccg gaacgtatgc
     6241 cacgacgtta ccagccgggc gcgaaggggt gatcaataaa gatattaatg gcgtggtgcg
     6301 ctgggacttc gcaccattgc aatcgctgga actggaagcg ggttacagcc gccagggtaa
     6361 cctgtatgcg ggcgataccc agaacaccaa ctctgacgct tacactcgct cgaaatatgg
     6421 cgatgaaacc aaccgcctgt atcgccagaa ctactcgctg acctggaacg gtggctggga
     6481 taacggcgtg accaccagca actgggtgca gtacgaacac acccgtaact cgcgtattcc
     6541 ggaaggtctg gcgggcggta ccgaagggaa atttaacgaa aaagcgacac aggatttcgt
     6601 cgataacgat cttgatgacg tgatgctgca tagcgaagtt aacctgccga ttgatttcct
     6661 cgttaaccag acgctgacgc tgggtacgga gtggaatcag cagcggatga aggacttaag
     6721 ttccaacacc caggcactga ccggaacgaa taccggtggc gctattgatg gcgtgagtgc
     6781 caccgaccgt agcccgtatt caaaagcaga aattttctcg ctgtttgccg aaaacaacat
     6841 ggagctgact gacagcacca tcgtaacgcc ggggctgcgt ttcgatcatc acagtattgt
     6901 cggcaataac tggagcccgg cgctgaacat atcgcaaggt ttaggcgatg acttcacgct
     6961 gaaaatgggc attgcccgcg cctataaagc gccgagcctg taccagacta acccgaacta
     7021 cattctctac agtaaaggtc agggctgcta tgccagcgcg ggcggctgct atctgcaagg
     7081 taacgatgac ctgaaagcag aaaccagcat caacaaagag attggtctgg agttcaaacg
     7141 cgacggctgg ctggcgggca tcacctggtt ccgtaacgat tatcgcaata agattgaagc
     7201 aggctatgtt gctgtagggc aaaacgcagt cggcaccgat ctctatcagt gggataacgt
     7261 gccgaaagcg gtggttgaag gtctggaagg atcgttaaac gtaccggtta gcgaaacggt
     7321 gatgtggact aataacatca cttatatgct gaagagtgaa aacaaaacca cgggcgaccg
     7381 tttgtcgatc atcccggagt atacgttgaa ctcaacgctg agctggcagg cacgagaaga
     7441 tttgtcgatg caaacgacct tcacctggta cggtaagcag cagccgaaga agtacaacta
     7501 taaaggtcag ccagcggttg gaccggaaac caaagaaatt agtccttaca gcattgttgg
     7561 gctgagcgcg acgtgggatg tgacgaagaa tgtcagtctg accggcggcg tggacaatct
     7621 gttcgacaaa cgtttgtggc gtgcgggtaa tgcccagacc acgggcgatt tggcaggggc
     7681 caactatatc gccggtgccg gggcgtatac ctataacgag ccgggacgta cgtggtatat
     7741 gagcgtaaac actcacttct gatgctaacg tcagattgtt gacaaagtgc gcgtcgttca
     7801 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaaa tcttgccaat tcaatatatt
     7861 gcaggaccat gtaggcctga taagcgtagc gcatcaggca gttttgcgtt tgtcatcagt
     7921 ctcgaatatg gtcgatatga aaactacgca tacctccctc ccctttgccg gacatacgct
     7981 gcattttgtt gagttcgatc cggcgaattt ttgtgagcag gatttactct ggctgccgca
     8041 ctacgcacaa ctgcaacacg ctggacgtaa acgtaaaaca gagcatttag ccggacggat
     8101 cgctgctgtt tatgccttgc gggaatatgg ctataaatgt gtgcccgcaa tcggcgagct
     8161 acgccaaccg gtgtggcctg cggaggtata cggcagtatt agccactgcg gggctacggc
     8221 attggccgtg gtttcccgtc aaccgattgg cgttgatatc gaagaaattt tttctgcgca
     8281 aacagcgacc gaattgacag acaacattat tacaccagca gaacacgagc gactcgcaga
     8341 ctgcggttta gccttttctc tggcgctgac actggcattt tccgccaaag agagcgcatt
     8401 taaggcaagt gagatccaaa ctgatgcagg ttttctggac tatcagataa ttagctggaa
     8461 taaacagcag gtcatcattc atcgtgagaa tgagatgttt gctgtgcact ggcagataaa
     8521 agaaaaaata gtcataactc tgtgccaaca cgattaattg acaacatctg gtacgattcg
     8581 cccgcagcca tcactgactg cgggcgaaag tgtaaagcag gtgccttacc actctgacct
     8641 gacaaccgga tatgcgggga ttgctccccg cacaacggct acttcttcgg ttcgtaagcg
     8701 agaacagcct taaactcaat attacgttcc tttacggtga actcacacag cgagtctccg
     8761 accagaagcg taaatcccag caccgtcaaa cacagcacta tgactgccac aagggcatat
     8821 ttcgtcagca tgtttatatt gcctccatgc gaagaaggga ctaccatccg agttgttggg
     8881 gctcggagtg gcagccccag gattgataga aatatctcct ggggcttcat ctttccagac
     8941 ctcagagtta acctgaaacc ggaaagcttc aggcacccgc cgttatctta cccttcctta
     9001 gcaaaatagc tgtttattta tacagtgaat cctgtttcgg acttctgtcg gcaaaactca
     9061 tcattgctct taaaacgaaa tcgcccgcag ccatcactga ccgcgggcga aagtgtaaag
     9121 caggtgcctt accatcctga cctgacaacc ggatatgcgg ggattactcc ccgcacaacg
     9181 gctacttctt cggttcgtaa gcgagaacag ccctgaactc aatattacgt tccttcaccg
     9241 taaactcaca cagcgagtct ccgaccagaa gcgtaaatcc cggtaccgtc aaacacagca
     9301 ctatgactgc cacaagggca tatttcgtca gcatgtttat attgcctcca tgcgaagaag
     9361 gaactaccat ccaaattgtc tggtttggag tggcagcccc agggttgata gaaatatctc
     9421 ctggagcttc atctttccag acctcagagt aaacctgaaa ccggaaagct tcaggcaccc
     9481 gccgttatct tacccttcct caacaaaata gctgtttatt tatacagtaa atcctgtttc
     9541 ggacttctca ctcctcattt aatcaactga agctgagccc gttttttctg catgcctcaa
     9601 tcgtcaaaat tcttcgccac cagccagccg tgccataact tgtcacattg caaaagtagc
     9661 caaattgctt cagtcaccat caccaaaaaa agaaacattc cagaacttcc gcatctcgcc
     9721 cagccgctcc gacttctata ctgaatagaa aactccaaca taagagaaac ctatgccatt
     9781 acccgatttt catgtttctg aaccttttac cctcggtatt gaactggaaa tgcaggtggt
     9841 taatccgccg ggctatgact taagccagga ctcttcaatg ctgattgacg cggttaaaaa
     9901 taagatcacg gccggagagg taaagcacga tatcaccgaa agtatgctgg agctggcgac
     9961 ggatgtttgc cgtgatatca accaggctgc cgggcaattt tcagcgatgc agaaagtcgt
    10021 attgcaggca gccgcagacc atcatctgga aatttgcggc ggtggcacgc acccgtttca
    10081 gaaatggcag cgtcaggagg tatgcgacaa cgaacgctat caacgaacgc tggaaaactt
    10141 tggctatctc atccagcagg cgaccgtttt tggtcagcat gtccatgttg gctgtgccag
    10201 tggcgatgac gccatttatt tgctgcacgg cttgtcacgg tttgtgccgc actttatcgc
    10261 cctttccgcc gcgtcgccat atatgcaggg aacggatacg cgttttgcct cctcacgacc
    10321 gaatattttt tccgcctttc ctgataatgg cccgatgccg tgggtcagta actggcaaca
    10381 atttgaagcc ctgtttcgct gtctgagtta caccacgatg atcgacagca ttaaagatct
    10441 gcactgggat attcgcccca gtcctcattt tggcacggtg gaggttcggg tgatggatac
    10501 cccgttaacc cttagccacg cggtaaatat ggcgggatta attcaggcca ccgcccactg
    10561 gttactgaca gaacgcccgt tcaaacataa ggagaaagat tacctgctgt ataaattcaa
    10621 ccgtttccag gcctgccgct atgggctgga aggcgtcatt accgatccgt acactggcga
    10681 tcgtcgacca ctaacggaag acaccttgcg attgctggaa aaaatcgccc cttctgcaca
    10741 taaaattggt gcatcgagcg cgattgaggc cctgcatcgc caggtcgtca gcggtctgaa
    10801 tgaagcgcag ctgatgcgcg atttcgtcgc cgatggcggc tcgctgattg ggctggtgaa
    10861 aaagcattgt gagatctggg ccggtgacta agccgggaat tgccttacag aagaatatcc
    10921 ctgacaatgg tgttttaact tacatttaac aatgttatga aacacccttt agaaaccttg
    10981 accaccgcag caggcatttt gctgatggct ttcctctctt gcctgctgct gcccgccccc
    11041 gcactggggc ttacgctggc acaaaaactg gtgaccacgt tccatctgat ggatcttagt
    11101 cagctttaca ctttattgtt ttgtctgtgg tttttagtgc tgggcgctat tgagtatttt
    11161 gttctgcgct ttatctggcg acgctggttc tcgctggcgg attaaacgtc gattatcgcc
    11221 gtacttcgcg gcatactttg cttattctcc ttcgcctaaa ggaacgttta tggataagca
    11281 atcactgcac gaaacggcga aacgcctggc ccttgagtta ccctttgtcg agctttgctg
    11341 gccttttggc ccggagttcg atgtttttaa aattggcggc aagattttta tgctgtcgtc
    11401 ggagctacgc ggcgtcccct ttatcaatct gaagtccgat ccacaaaaat ccctgttaaa
    11461 tcagcaaata tacccaagca ttaagccagg gtatcacatg aataaaaagc actggatttc
    11521 ggtgtatccc ggcgaggaaa tctccgaagc gttacttcgc gatctgatca acgattcgtg
    11581 gaatctggtg gttgatggtc tggctaaacg cgatcaaaaa agagtgcgtc caggctaaag
    11641 cggaaatcta tagcgcattt ttctcgctta ccatttctcg ttgaaccttg taatctgctg
    11701 gcacgcaaaa ttactttcac atggagtctt tatggatatc atttctgtcg ccttaaagcg
    11761 tcattccact aaggcatttg atgccagcaa aaaacttacc ccggaacagg ccgagcagat
    11821 caaaactctc ctgcaataca gcccatccag caccaactcc cagccgtggc attttattgt
    11881 tgccagcacg gaagaaggta aagcgcgtgt tgccaaatcc gctgccggta attatgtgtt
    11941 caacgaacgt aaaatacttg atgcctcgca cgtcgtggtg ttctgtgcaa aaaccgcgat
    12001 ggacgatgcc tggctgaagc tggttgttga ccaggaagat gctgatggcc gctttgccac
    12061 gccggaagcg aaagccgcga acgataaagg tcgcaagttc ttcgccgata tgcaccgtaa
    12121 agatctgcat gatgatgcag agtggatggc aaaacaggtt tatctcaacg tcggtaattt
    12181 cctgctcggc gtggcggctc tgggtctgga cgcggtaccc atcgaaggtt ttgacgccgc
    12241 catcctcgat gcagaatttg gtctgaaaga gaaaggctac accagtctgg tggtagttcc
    12301 ggtgggtcat cacagcgttg aagattttaa cgctacgctg ccgaaatctc gtctgccgca
    12361 aaacattacc ttaaccgaag tgtaattctc ccttgccggg catctgcccg gctatttccc
    12421 ctcagattct cctgatttgc ataaccctgt ttcagccgtc atcataggct gctgttgtat
    12481 aaaggagacg ttatgcagga tttaatatcc catgttgaag atttagcggg tattgagatc
    12541 gatcacacca cctcgatggt gatgattttc ggtattattt ttctgaccgc cgtcgtggtg
    12601 catattattt tgcattgggt ggtactgcgg accttcgaaa aacgtgccat cgccagttca
    12661 cggctttggt tgcaaatcat tacccagaat aaactcttcc accgtttagc ttttaccctg
    12721 caggggatta tcgtcaatat tcaggcggta ttctggctgc aaaaaggcac cgaagcggca
    12781 gatattctga ctacctgcgc gcagttgtgg atcatgatgt atgcgctgct ttcagtcttc
    12841 tcgctgctgg atgttatttt gaatctggcg cagaaattcc cggcagcatc tcagttaccg
    12901 ctgaaaggga tatttcaggg gattaaactg atcggcgcga ttctggtcgg cattttgatg
    12961 atctcgctgc tgattggtca gtcgccagcg attctgatca gcggtcttgg tgcaatggct
    13021 gccgtgctga tgttggtatt taaagatccg attcttggtc tggtggcagg tattcagctt
    13081 tccgcgaacg atatgctgaa actgggcgac tggctggaga tgccgaaata cggcgcggat
    13141 ggcgcggtga tcgatattgg gttaaccacc gtcaaggtgc gtaactggga caataccatt
    13201 accactattc ccacctggtc tctggtttct gactccttta aaaactggag cgggatgtca
    13261 gcatctggcg ggcgacgtat taagcgcagt atcagtattg atgtcaccag tattcgtttt
    13321 cttgatgaag acgaaatgca acgtctgaat aaagcgcatt tgttaaagcc ttatttaacc
    13381 agccgccatc aggaaattaa tgagtggaat cgccagcaag gttctacgga gtcggtatta
    13441 aacctgcgcc gaatgaccaa tattggaacc tttcgtgcct atctgaacga atatctgcgt
    13501 aaccatccgc ggattcgtaa agatatgacc ttaatggtac gccaactggc tccaggtgat
    13561 aacggtttac cgctcgagat ctatgcgttt accaacaccg tggtgtggct ggaatatgaa
    13621 agcattcagg ctgatatatt cgatcacata tttgcgattg tcgaagagtt tggtctgcga
    13681 cttcatcagt cgccaaccgg caatgatatt cgctctctgg cgggtgcatt taagcagtaa
    13741 ttaaaaaaac cgctctcatc gaatggatga gagcggacat cgttttgtcg gatgtagcgt
    13801 gaatgcctta tttccgacgc agcgttttaa atgctacaaa caggaataca atccacaccg
    13861 gcagcaggat cgctgacaag cgcatatcat ccatcgtgca catcagcagc aaaatcatgg
    13921 cgaggaaggc gatgcaaaga tagttgccga acggatagag cagcgcctta aactgtgttt
    13981 cacgcccctg acgtcgcatc gctgcacgaa aacgcagatg cgccagacag atcataatcc
    14041 agttcaacag cagcgttgct accaccagcg ccatcagcag accaaacgct ttttgcggca
    14101 gcagatagtt gattaacacc accagcgaag tgatcgctcc ggaaagcatc agcgagttaa
    14161 tcggcacgcc gcgacggcta acacgggtaa ggaacttcgg cgcattaccc tgcacagaaa
    14221 ggccaaacag catgcggctg ttagagtaaa ccccgctgtt atacactgac agcgatgcta
    14281 ccagaatgac gaagttcagc gcagaagcta ccacgttgct gtcgagatta tggaaaatca
    14341 tcacaaacgg gctactgttg gatttcactt ccacccacgg atagagcgcc agtaaaacca
    14401 ccagtgaacc gatgtaaaac agcaggatgc gatacaccac ctgatttacc gcttttggaa
    14461 tgcttttttc cggatcgcgc gcttcagcgg cagtaatccc aatcagctcc agaccgccga
    14521 aggagaacat aattaccgcc agcgacaaaa tcagcccatt ccagccggtg gcgaagaaac
    14581 caccgtagcg ccagaggttg tcgatactgg ctttctcgcc gccgtgacca gaaaacagca
    14641 gccacaggcc aaagccgatc ataccgatga ttgccagcac tttaatcagc gcaaaccaga
    14701 actcggtttc gccatataag cgcacgttca ccaggttaac ggcgttgatg ataataaaga
    14761 aggcggcagc ccaaatccac gttggaacat ccgggaacca gtactgcata tagatgcccg
    14821 cagcggtcag ctctgccatt cccaccagca cgaacatcac ccagtagttc cagccggaga
    14881 ggaacccggc gaacggtccc cagtatttat aggcaaagtg ggcaaatgaa ccggataccg
    14941 gctcttcgac caccatctcg ccgagctggc gcataatcag gaaagcgatg atcccggcga
    15001 cgccgtagcc cagcaataca gccggacccg ccatctgaat cgccgggcca atgccaagaa
    15061 acagaccagt accaattgcg ccacccagcg caatcagttg aatatgacgg ttatgtaatc
    15121 cgcgatgaag cgtcggctct tgattcgacg cagtatcttc cgatacgttt gacgcgtttt
    15181 tcacgccttt cccctgtgtg tcttttttgt tgaggggcac cttttaacat ttagtgccca
    15241 tcgtcgcaag acacaatcca cacggttaaa ccgggtatcc tgcttttatt tccgtacccg
    15301 atgtcggtgc agccacatca gcttatacgc tgccgggata ataaacagcg acagcaaagg
    15361 cgcggtgatc atgccgccaa tcatcggcgc ggcaatccgg ctcatcacct ctgatcctgc
    15421 ccccgttccc cacagaatcg gcagcagacc agcgataatc accgccaccg tcatcgcttt
    15481 cgggcgcacg cgcaggaccg cgccgtgatg taacgcctca tccagcttct gctcgctgaa
    15541 tgtttgcgga ttattcaacg acggctcggc ctctatggcg tgacgtaaat acatcagcat
    15601 caccacgcca aattcggcgg cgaccccggc aagggcaata aaaccggtgc ccgtcgccac
    15661 agaaagatga aagcccatcc accacagcaa ccagatgccg cccaccagcg caaacggtac
    15721 gctgctgata atcagcaacg cttcgcccac ccgacggaac gccagataca acagcacgaa
    15781 gatgatcatc aacgtcatcg gcaccatgag tttcagctta tggttggcgc gctccagtag
    15841 ctcgaactgc ccggagaatg ccacgctggt gcccggtttt aactgcactt tttcagctat
    15901 cgctttttgc aaatcgtgaa ccaccgacac catgtcacga tcgcgggcat cgatataaat
    15961 ccagctcgtc gggcgcgcat tctcggtttt cagcatcgac ggtccggtag agactttaac
    16021 gtcggccacg tctgccaggg tgatttgctg cttcatcggc gtcaggatcg gcaactggcg
    16081 cagtgcctgc ggactatcgc gccagctttg cggataacgc agattaattg gataacgggc
    16141 aatcccttcc accgtttcgc caaccatcgc cccgcccacc gcagaagtca caaacaactg
    16201 cacatccgcc accgtcatac cgtaacgtgc ggctttttca cggttaatct caacgttgat
    16261 atagcgccca ccttccagcc gctcggcaag cgcagaagcc acgcctggca ccgttcgtgc
    16321 cacctcttca atttgctccg ccatcgcgtc gatatccgcc agcacagtgc cggaaacttt
    16381 tatgccgata gggcttttaa tgccggttga gagcatatcg atacggttac gaattggcgg
    16441 cacccacaga ttcgccagcc ccggcagacg cacggtgtta tccagttcct caatgatttt
    16501 gtccattgtc atgcctggcc gccactgctc ctgcggctta agctggatgg tcgtttctac
    16561 catctccagc ggagcagaat cggtggcggt ttccgctttc ccggttttgc caaatacccg
    16621 cgccacttca ggtacgctca taattagctt gtcggttttt tgcagcatac tcgccgcctc
    16681 tgctgcggaa atccccggca gcgtcgatgg catatacaac aagtcgcctt cattgatctg
    16741 cggtaaaaat tccccgccaa ctttattgag cggccagaga accgtcagca ccgaaagcgc
    16801 tgccaccaac agcgtggttt tcggccagtg cagtactttc agcaacagcg gatgataaac
    16861 acgaatcaaa aagcgattga gcgggttact gctttccggc ggaattttgc cacggatcca
    16921 gtaacccatc aggataggga tcactacgat cgccagcagc gccgcacccg ccatcgcata
    16981 cgttttggtg aacgccagcg ggccaaacag acgcccttcc tgcccttcca gggtgaagat
    17041 cgggataaac gacagcgtga taatcagcag actgataaac agcgccggcc ccacttcaac
    17101 agatgcattg gtaatcacct gccagcgcgt tttattatcc agcgtggcgt caggatgctg
    17161 gtgctgccac tcttccagcc gtttatgtgc attctcgatc atgacgatag cggcatcgac
    17221 catcgccccg acggcaatcg caatgccacc caacgacata atattggcat tcagcccctg
    17281 gaagtgcatg acaataaaag caatacacaa ccccagcggc aacgaaataa tcgccaccag
    17341 cgccgagcgc acatgccaga gaaacagcgc acagactacc gccaccacaa taaactcttc
    17401 cagcaacttg ccgctgaggt tgtcgatggc ccggtcaatg agctggctgc gatcgtatgt
    17461 tgtaactatc tccacgcctt ccggcagact gcttttcagc gtttccagtt tgtccttcac
    17521 ggcggcgatc acttcacggg cgtttttgcc ggagcgtagg atcaccaccc cgcccacaac
    17581 ttcgccgttc agttcggcaa tgccccggcg catctccggg ccaacctgga tcttcgcaac
    17641 atcacgcaga taaacaggca cgccattttc actggctttt aaaacgatgt gattaaagtc
    17701 gtcgagcgtt tgtagatagc cgctggcgcg caccatatat tctgcttccg ccagttcgat
    17761 tgacgaaccg cccgcttcct ggtttgaagc atccagcgcg ctttttactt cggcgagact
    17821 gatgccatac tgcgccaggc gctgaggatc gataaccacc tgatactctt tcaccacgcc
    17881 gcccaccgac gccacttccg caacgtcagg gatggttttt agctcatatt tgagaaacca
    17941 gtcctgcaat gagcgtaaat cggccagatc gtgcttaccg ctgcgatcca ccagtgcata
    18001 ttcatagatc cagccaacac ccgtggcatc tggtcccagc tcggcgctga ctcccgcagg
    18061 cagcttaccc tgtacctggt tgaggtactc cagcacccgc gaacgcgccc agtacggatc
    18121 ggtaccatct tcgaaaatga catacacata agagtcgcca aactgcgaga aaccacgcac
    18181 agtcttcgcg ccaggcaccg acaacatggt ggtagttagc ggataagtca cctgattttc
    18241 aacgatttgc ggtgcctgac cgggatagct ggttttaata atcacctgca catcggagag
    18301 atccggcaac gcatccactg gcgtgttaat gatggtccag gtgccccaga tactcagaaa
    18361 taacgcgccc atcagcacca aaaaacggtt cgccaccgag cgacgaataa tccattcaat
    18421 cattggttat tccctcaatg cgcatgggta gcactttcag agcgcatccg ctccagtgcg
    18481 ccagaaatat tggcttcgga atcaatcagg aacaggccgc tggaaaccac cttttcacct
    18541 tccgccagac cagagcgtaa tgcggtgaca ccttgcgatg cctggaaaac ggcaacgcgt
    18601 tttggtacaa agcgcccgtc ggcatcaacg gtaatcaccc gctgttcgct gccggtatca
    18661 atcagcgcct gtgacggaat gagcagcatc ggttcgctgg cggtattgag ttgcaaccag
    18721 gcgttcattc ccggttttag cgcctcgtcg gcgttgtcga cttccagacg cagctgcagc
    18781 gtgcgggtcg cggcatccac gccaggtagt agcgtccatt tgcggatggt gagtgtttta
    18841 tcaggtcgcg ccggaacggt gagcgtaaac tgcgaggcat ctttcaccag ccaggcgatg
    18901 gactccggga tcgcagcagt gacccacacc gggtccatac cctgaatttt cgctaccacg
    18961 ttatctttgg cgatattcat tcccgcgcgc agatcaaacg cggtgatcac gccatcaatg
    19021 ggtgctttga gcgtaaagcg agtctggatt ttttgcgtgg cgatcaggcg gcgaatatcc
    19081 gcctccggca ttcccgccag tcgcagccgc tcaagaatgc cttcggtctg ggtcgccgta
    19141 ccgccggttt cgcgcagcag taaatactca ctctgcgctt ccacccagtc gggaatggtc
    19201 aggtcgagaa gcggcgcgcc cttttgtact ttatcgccca cggtaagcgg atacaccttg
    19261 tcgataaacc cggcggcgcg agcctgcaca atggcatact gatactcgtt gtaactgaca
    19321 ttcgccggga aactctgggc aaaagtcagc ggcccgcgcg tgacggtagc cgttttcacc
    19381 cccagattct gcgtctgagt cgggtcaatg cgcacaccag acgcagaact ctcttcatcg
    19441 gcatatttcg gcaccagatc catatccata aacggcgatt tacctggttt atcgaaccgc
    19501 gtattgggat acatcgggtc gtaccagaat aagattttac gttctgcggt cgacgttttt
    19561 tctgcaggcg gttccgcctt tgcaaaccag gtaaaacctg ccgcagaaat aataccgccc
    19621 gcgatcatgc tgccgataat aagcgcgatt tttttcatct cattaaacct gggttactgg
    19681 ctgactttaa tatcctgtaa taaagaaagg ttgccctgct ggacaaaatt aaacgccact
    19741 ttgtcgccgg ttttaattcc actcattttc gtctgcgggg tgatggtaaa gcgcatggtc
    19801 atctccggcc agttcacggc agcaatcgga tcgtgatgga tggtgatttt tttgctttcc
    19861 agatcaaaac cttttaccac gccggtggca ctaatgacct gtggttgtgc ttcgctcatg
    19921 gtttcatgat gatgttcgtt agcctgggca ttaaagccaa taacggtaaa cagactgaac
    19981 attgcgactt gcagtgcttt tttcatttat tttctcctgg agttaaaaat tattgctgcc
    20041 aaccgccacc aagtgcggta tataaagaaa tttcgttgac ctgacgggca taattcaggt
    20101 caagtacggt ttgtcgggtt gcaaataaag aacgctcggc atccagcact tccagataac
    20161 ttactgcgcc gtgctgatat aatgcccgcg cccgttgcaa agtaatttgc agcgatgcca
    20221 gataacgctg ctgggcgcta atttgatcgt tcaggctttg acgtaatgcc agcgcatctg
    20281 ccacttcttt aaaggcgttc tggattttct gttcataatt cactaccgac tgctgctggc
    20341 gaatttcggc gatatccaga ttggcctggt tgcgtccggc attaaaaatg gggatctcaa
    20401 ttttaggaat aaaattccac atcccgctgc tggcgttaaa taatgacgac agatcgctgc
    20461 tggcggttga tattccgctg gtcaggctga tagacgggaa aaatgccgcg cgtgcagcac
    20521 caatattggc attagccgcc attaacgcat gttcagcttc cataatatcc gggcgctgca
    20581 ataagatttg cgacgacaag cccgccggta atttaacgct ttgcaggctg tcgctgttta
    20641 ctgtctgcgc ttgcggcagc ttgccgtagc ttcccagtaa cagttgcaat gcattattcg
    20701 cctgcgccag ttccccctga cgtttagcga tgtcgctgcg ggtactttca atcaccccgc
    20761 gagcctgttc cagcgccaga acattactgc tacctgtcaa cagttgtttt tcgacaaacg
    20821 catatgactg ctgataatta cgcagcgttt cttcggctat ttgcaattgc gcatacgcca
    20881 gctgctgatt gaaatagctt tgcgcgacat tagaaaccag cagaatatgc accgcgcgct
    20941 gagcttcctc agtggctaaa taattttgtc gctcggcttc gctcatgttc tttaagcgcc
    21001 cgaaaaaatc gaggtcaaag ctggcgttaa ggccagtaga aaactcccgc gtcgtggctg
    21061 aatcgccttt aagattgccg ctccagctgc cgctgccctc accattgagc tgtgggtagc
    21121 ggtcggcatc agtcagacga tattgcgccc gtgcctcctg cactttcagc gtcgccatgc
    21181 gcaaatcccg gttattctcc agcgcctcgc taatcagcgt cttcacctga ttatcaacaa
    21241 aaaaggtgcg ccagcccgcg ttctgatagt tatctgctgc gttaaccagg ccgttctggc
    21301 tgagtgagaa ctgctgcggt acgggcattg ccgggcgctg ataatccggt gccagtgaac
    21361 aaccggttag cgcaagggcc acacaaaatg gcagaagttt acaaggagac ataggctcat
    21421 aatttctggt gattttatac cgccaacttt actcgccagg ctctgatttt ccggtgacag
    21481 gaaaatgaca aaattgtcat tttgccaata agcgattgcc atctgatccc gctactctag
    21541 aattgcccgg gcaacatgcg gaggaaatat gaaactgttg attgtcgaag atgaaaagaa
    21601 aaccggagaa tacttgacca aagggttaac cgaagccggt tttgtggtcg atttggccga
    21661 caacgggctg aatggctacc atctggcgat gaccggagat tatgacctga taatcctcga
    21721 tattatgctg ccggacgtga acggctggga tatcgtgcgc atgttgcgct ccgccaataa
    21781 agggatgccg attctgttgc ttaccgcgct tggcaccatt gaacatcgcg tcaaggggct
    21841 ggagttgggg gccgatgact atctggtgaa gccgttcgcg tttgctgaac tgctggcgcg
    21901 ggtgcgcact ctcctgcggc gcggggcggc ggtgattatc gaaagtcagt ttcaggttgc
    21961 cgatttgatg gtcgatctcg tcagccgcaa agtcacccgc agcggcacgc gcatcactct
    22021 gaccagtaaa gagtttactc tgctggagtt ttttctccgc catcagggcg aagtgctgcc
    22081 ccgctcgctt atcgcctcgc aggtatggga catgaatttt gacagcgaca ctaacgccat
    22141 tgatgtggcg gtgaagcggc tacgcggcaa aatcgacaac gactttgagc cgaagctgat
    22201 tcaaaccgtg cgcggcgtgg gttacatgct tgaggtgccg gatggtcagt aagccatttc
    22261 agcgcccgtt ttcgctggca acccgcctga ccttttttat cagcctggcc accatcgcgg
    22321 cgtttttcgc ctttgcatgg atcatgatcc actcagtaaa agtgcatttt gccgagcagg
    22381 atattaatga tttaaaagag attagtgcca cccttgaacg cgtactaaat caccctgacg
    22441 aaacgcaagc ccgacgctta atgacgctgg aagatatcgt cagtggttat tccaatgtgt
    22501 tgatttccct ggcagatagc cacggtaaaa cggtgtatca ctcccccggt gcgcctgata
    22561 tccgcgagtt tgcgcgcgat gccatacccg ataaagacgc gcggggcggc gaggtgtttc
    22621 tcctctccgg cccgacgatg atgatgccag gccacggtca cgggcatatg gaacacagca
    22681 actggcggat gatcagtttg ccagttggcc cactggttga cggcaaaccg atttatacgc
    22741 tctacatcgc gctttcgatc gattttcatc ttcattacat aaatgatttg atgaataaac
    22801 ttattatgac cgcatcggtc atcagcatcc tgatcgtctt tatcgtgcta ttggcggttc
    22861 ataaaggtca cgcgccgatc cgcagcgtca gccgtcaaat ccagaatatc acctcgaaag
    22921 atctcgacgt ccgccttgac ccgcagaccg tgccgattga gctggaacag ctggtactgt
    22981 cgttcaacca tatgatcgag cgtattgagg atgtctttac ccgccagtcc aatttctcag
    23041 cggatatcgc ccacgaaatt cgcacaccaa ttactaatct cataacgcaa acggaaatcg
    23101 ccctcagcca gtcgcgcagc cagaaggagc tggaagatgt gctctactct aatctcgaag
    23161 agctgacgcg aatggcgaaa atggtcagcg atatgctgtt tctcgcccag gccgataaca
    23221 accagttaat ccccgaaaag aaaatgctca acctggcgga tgaagtcggc aaagtgttcg
    23281 attttttcga ggctttagcg gaagatcgcg gcgtggagct gcagtttgtt ggcgacgagt
    23341 gccaggttgc gggcgatccg ctgatgctgc gtcgggcgtt aagcaacctg ctttcgaacg
    23401 ccctgcgtta tacgccaccc ggagaggcta ttgtagtgcg ctgccagacg gtcgatcatc
    23461 tggtgcaagt tatcgtcgaa aaccccggta cgcccattgc gcccgagcac ttaccgcgat
    23521 tgtttgaccg tttctaccgc gttgacccct cccgccaacg aaaaggcgaa ggtagcggca
    23581 ttgggctggc gatagtgaaa tcaattgttg tcgcgcataa aggcacggtt gcggttacgt
    23641 caaatgcgcg ggggacaagg tttgtgattg tgttgccgga gagggggtga ttattaagtt
    23701 caaattcagg gcgagggttt acggcgtttt tcgctaaatt tccat
//
LOCUS       DBFIFM010000060        23539 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.060, whole genome shotgun sequence.
ACCESSION   DBFIFM010000060 DBFIFM010000000
VERSION     DBFIFM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.060"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..306)
                     /gene="chbB"
                     /locus_tag="N7C74_002020"
     CDS             complement(<1..306)
                     /gene="chbB"
                     /locus_tag="N7C74_002020"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HGU2431892.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     "
     gene            complement(606..944)
                     /gene="osmE"
                     /locus_tag="N7C74_002021"
     CDS             complement(606..944)
                     /gene="osmE"
                     /locus_tag="N7C74_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HGU2431893.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1146..1973
                     /gene="nadE"
                     /locus_tag="N7C74_002022"
     CDS             1146..1973
                     /gene="nadE"
                     /locus_tag="N7C74_002022"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HGU2431894.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREACIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2203..3090
                     /gene="cho"
                     /locus_tag="N7C74_002023"
     CDS             2203..3090
                     /gene="cho"
                     /locus_tag="N7C74_002023"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HGU2431895.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSQEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3050..3625)
                     /gene="ves"
                     /locus_tag="N7C74_002024"
     CDS             complement(3050..3625)
                     /gene="ves"
                     /locus_tag="N7C74_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HGU2431896.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADHFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3828..4313)
                     /gene="spy"
                     /locus_tag="N7C74_002025"
     CDS             complement(3828..4313)
                     /gene="spy"
                     /locus_tag="N7C74_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HGU2431897.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4643..5611)
                     /gene="astE"
                     /locus_tag="N7C74_002026"
     CDS             complement(4643..5611)
                     /gene="astE"
                     /locus_tag="N7C74_002026"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HGU2431898.1"
                     /translation="MDNFLALTLTSKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFKKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5604..6947)
                     /gene="astB"
                     /locus_tag="N7C74_002027"
     CDS             complement(5604..6947)
                     /gene="astB"
                     /locus_tag="N7C74_002027"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HGU2431899.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNKASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVVRQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTQEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(6944..8422)
                     /gene="astD"
                     /locus_tag="N7C74_002028"
     CDS             complement(6944..8422)
                     /gene="astD"
                     /locus_tag="N7C74_002028"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HGU2431900.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFAERQAVVERFAGLLERNKGELTAIIARETGKPRWEAATELTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGCETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRVLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVFTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8419..9453)
                     /gene="astA"
                     /locus_tag="N7C74_002029"
     CDS             complement(8419..9453)
                     /gene="astA"
                     /locus_tag="N7C74_002029"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HGU2431901.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9450..10670)
                     /gene="astC"
                     /locus_tag="N7C74_002030"
     CDS             complement(9450..10670)
                     /gene="astC"
                     /locus_tag="N7C74_002030"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HGU2431902.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNESVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNIINHRYGLFNEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAVACEHFVSRGSS"
     gene            11116..11922
                     /gene="xthA"
                     /locus_tag="N7C74_002031"
     CDS             11116..11922
                     /gene="xthA"
                     /locus_tag="N7C74_002031"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HGU2431903.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDEEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12089..12799
                     /gene="ydjX"
                     /locus_tag="N7C74_002032"
     CDS             12089..12799
                     /gene="ydjX"
                     /locus_tag="N7C74_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HGU2431904.1"
                     /translation="MNAERKFLFACLIFALAIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPFLGTLLSLIAATLASSCSFLLARW
                     MGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRNPLTHPKNEG"
     gene            12804..13481
                     /gene="ydjY"
                     /locus_tag="N7C74_002033"
     CDS             12804..13481
                     /gene="ydjY"
                     /locus_tag="N7C74_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HGU2431905.1"
                     /translation="MLQHYSVSWKKGLTALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKTYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPANNTLAT
                     VTFKITE"
     gene            13496..14203
                     /gene="ydjZ"
                     /locus_tag="N7C74_002034"
     CDS             13496..14203
                     /gene="ydjZ"
                     /locus_tag="N7C74_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HGU2431906.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGSLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            14203..14751
                     /gene="ynjA"
                     /locus_tag="N7C74_002035"
     CDS             14203..14751
                     /gene="ynjA"
                     /locus_tag="N7C74_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HGU2431907.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQELTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            14761..15927
                     /gene="ynjB"
                     /locus_tag="N7C74_002036"
     CDS             14761..15927
                     /gene="ynjB"
                     /locus_tag="N7C74_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU2431908.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTDWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALNEAPNDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTFRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            15900..17435
                     /gene="ynjC"
                     /locus_tag="N7C74_002037"
     CDS             15900..17435
                     /gene="ynjC"
                     /locus_tag="N7C74_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HGU2431909.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCTRLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGFTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWCTWRRTIPRVDGVRKP
                     ATPLLPGITLASFLPLTSVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNMDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAALALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            17435..18088
                     /gene="ynjD"
                     /locus_tag="N7C74_002038"
     CDS             17435..18088
                     /gene="ynjD"
                     /locus_tag="N7C74_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGU2431910.1"
                     /translation="MLCVKNVSLRLPESCLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNTRRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            18155..19462
                     /gene="ynjE"
                     /locus_tag="N7C74_002039"
     CDS             18155..19462
                     /gene="ynjE"
                     /locus_tag="N7C74_002039"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HGU2431911.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRSSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAARGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(19471..20091)
                     /gene="ynjF"
                     /locus_tag="N7C74_002040"
     CDS             complement(19471..20091)
                     /gene="ynjF"
                     /locus_tag="N7C74_002040"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGU2431912.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKYQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            20178..20585
                     /gene="nudG"
                     /locus_tag="N7C74_002041"
     CDS             20178..20585
                     /gene="nudG"
                     /locus_tag="N7C74_002041"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HGU2431913.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(20551..20823)
                     /gene="ynjH"
                     /locus_tag="N7C74_002042"
     CDS             complement(20551..20823)
                     /gene="ynjH"
                     /locus_tag="N7C74_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HGU2431914.1"
                     /translation="MSRALFAVVLAFPLIALANPHYGPDVEVNVPPEVVSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            21059..22402
                     /gene="gdhA"
                     /locus_tag="N7C74_002043"
     CDS             21059..22402
                     /gene="gdhA"
                     /locus_tag="N7C74_002043"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HGU2431915.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKSSRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(22519..>23539)
                     /gene="ynjI"
                     /locus_tag="N7C74_002044"
     CDS             complement(22519..>23539)
                     /gene="ynjI"
                     /locus_tag="N7C74_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU2431916.1"
                     /translation="NHPGSEKYSFNGWEIFNSNFERMIKENKTMLLCKWGFYLTCVVA
                     VMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQAKGIEPL
                     SIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDIISKRRES
                     LEEQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVFPSDYISD
                     CANHSSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKAHELFESE
                     EDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIRNVPWGAS
                     SVKYS"
BASE COUNT         5659 a         6201 c         5965 g         5714 t
ORIGIN      
        1 ttttttaatc gctgcaaccg cagccttaag cacgcctaaa ccatcgactt tgccataaag
       61 cagcgagtca attacttcaa ccggtttgtt gggtaacaaa cgctggattt cgggcaacat
      121 ataagcaatc tgcggcccta ataacacgac atcggcattc tgaccttttt caccagccag
      181 tgtttccgga aatgcttcaa taatgaccgg aacttcatat ttttctgcct gtgcgcgcat
      241 ttttgatacc agtaaagagg tagacatgcc cgcagaacaa aacagataaa tgtgtttctt
      301 ttccataaaa ctgccctcat cgacgattat ctgtcagcca gacactccgc aagccttaac
      361 ctgcttccat gctctgggta acttgcgaaa ccaacatgac tttttttgtg gctgaaagga
      421 gtatacggta tagacctgag caatagtatt tctttgacct tataaattgt ctctccttga
      481 cctgcggtta tgaccctctt cacatttcgg gcaaataatt cgcgaagata attaagaact
      541 caggccaaaa aaaaaccggc gcaatggccg gtttccgttg ttactcaagc tttcagacga
      601 agtgattact tcgcagcctg tggatcagtg tcgtattcag cacaggtctg ataaccggag
      661 ttgatgacat gtccggtatc atctaacgcg acaaagtagg tttctgcttt accatcacgt
      721 tgacccagga tgtaggtctg gcaagtacca cgagcatgga tcatgctcac ttcagacgaa
      781 ggtttacccg caatttgtgc aacctgcgcc cggctcatgc cttttttgac gtctttcacc
      841 acaggctgta caaactggtc tttggtacga tcataagccg tacaacccgc cagcatggtt
      901 aataccgccg ctgcactcag aattcctgcc atattcttgt tcatattccg tcctattgtt
      961 tatcagcgtg ttagataagc ctggaataca ttgggcgctt tttcaagccc atgaacgaaa
     1021 cggctccgct ttcagaggat tcctgtatga cgttttaacc accattcagc ccgctgtcgc
     1081 ttgtcgtttc agtagcaacg ggttagcttt aaggaagttt tgtcttttct gtctggaggg
     1141 gttcaatgac attgcaacaa caaataataa aggcgctggg cgcaaaaccg cagattaatg
     1201 ctgaagagga aattcgtcgt agtgtcgatt ttctgaaaag ctacctgcaa acttatccgt
     1261 tcattaaatc actggtgctc gggatcagcg gcggtcagga ctccacgctt gccggtaagc
     1321 tgtgccagat ggcgattaat gagctgcgcc aggaaaccgg caacgaatca ctgcaattta
     1381 ttgccgtacg cctgccctat ggtgttcagg ccgacgaaca agattgccag gatgccattg
     1441 cctttattca accggatcgc gtattaaccg ttaatatcaa gggcgcggta ttggctagcg
     1501 agcaggcatt gcgggaagca tgcattgaac tgagcgattt tgtccgtggc aatgaaaaag
     1561 cgcgtgagcg gatgaaagca caatatagca ttgcgggtat gaccagcggt gtcgtggtgg
     1621 gcaccgatca tgcagcagaa gccattaccg gattcttcac taaatatggt gacggcggta
     1681 cggacattaa tccactgtat cgtctcaaca aacgtcaggg taaacagtta ctggcagcat
     1741 taggttgccc ggaacacctt tataagaaag cgccaacggc cgatctggaa gatgatcgcc
     1801 cttctctgcc agatgaagtg gcactcggcg tgacctatga caatatcgac gactatctgg
     1861 aagggaaaaa cgtacctcaa caggtcgcca gaacaataga gaactggtat ctgaaaaccg
     1921 aacataaacg ccgtccgcca attaccgttt tcgatgattt ctggaaaaag taataatttg
     1981 cacctattgg attgtgcaaa aaagagtaat ttgttcacgc cggatgcggc gtgaacgcct
     2041 tattcgacct acaaaactat gcaaattcaa tacattgcag gagtcgaata ggcctgacag
     2101 gcgtagcacg tcagacggtg taacctttgt catcgacccg cctctttttt aatcgcttcc
     2161 cggctgttac actggataga taaccagcat tcggagtcaa cagtggtacg gcgtttaact
     2221 tctccgcggc tcgaatttga agctgcggca atttatgaat atcccgaaca tttacgttca
     2281 ttccttaatg acttacccac ccgaccaggg gtgtatctgt ttcatggcga aagtgacacc
     2341 atgccgctct atatcggcaa aagcgttaat atccgcagtc gggtgctttc tcatttacgc
     2401 accccggatg aagccgccat gctacggcaa tcccggcgaa ttagctggat ctgcaccgca
     2461 ggcgaaatcg gcgctctgct ccttgaagcg cgattaatca aagaacaaca gccgctgttt
     2521 aataaacggt tgcgccgcaa tcgccagctc tgtgccctgc aattaaatga aaagcgcgtc
     2581 gatgtggtgt atgccaaaga ggtggatttt tcacgagccc ccaacctgtt tggcctgttt
     2641 gccaataggc gcgcagcttt gcaagcattg cagagcatcg ctgatgaaca aaaactttgt
     2701 tatggtctgt tgggactgga accgttaagt cgcggtcgtg cgtgttttcg ttcagcgcta
     2761 aaacgttgcg ccggagcatg ctgcggtaaa gagagccatg aggaacatgc gctacgcttg
     2821 cgccagtctc tggagcgttt gcgggtggtg tgttggcctt ggcaaggggc ggtggcgctg
     2881 aaagaacagc acccggaaat gactcaatat catattattc aaaactggct gtggctgggg
     2941 gcggttaatt cgcaggaaga agcgacaacg ttaattcgga cacccgccgg gtttgatcac
     3001 gacggttata aaattctttg taagccgctg ctttccggta actatgaaat tactgaactt
     3061 gatccggcga atgaccagcg agccagttga tttcactgaa caacagttta ccttgcggtt
     3121 gtaaaagccg cagggtgtgc ctgccatcga accagcacgc gccctggtcg gtagtgagca
     3181 gtttgtcgcc cagttgccac gccccgttaa tcacaaacac caccccgccg cgtgagccaa
     3241 aggtagtaaa cgtacgttcg gcaattctga ctttcgcctt acagacatcc aggcgcgtca
     3301 taatattgaa atccatcgac atctgccctg ccgtcagttt cgcttttacc acctggtccg
     3361 ccgcaaaggc aaaaggctgt aacggtttta aggtatggtt aaagtggtct gcgctttcaa
     3421 ggaacatctc gccgccttcc agcaacgtca ctatcctttc cataccagga aacaaagaaa
     3481 actccccatt cgccgcgatg gatgcaatgc tggcacgcca gtaaaaatca cgttttgccg
     3541 gtgggaaagt gcaaatttcg cgcgtttcac cggcagcgtt tcgccacaaa ttcaccgaca
     3601 ttttacgcat atcaaagtat tccatactcg ctcccggccc gttcttttca ttgttatcgt
     3661 tcaggtaacc gtggatggtt atcggcaact cgggtgattt acgttagtgg tgatcaggct
     3721 ttttttacaa ctgtttgaga agagaaaaga aaaccgccga tcctgtccac cgcattactg
     3781 caaggtagtg gacaagaccg gcggtcttaa gttttttggc tgaaagatta ttcagcagtt
     3841 gcaggcattt taccttttgc cgctggacgt tctgtcagac gcttctcaaa attagcatta
     3901 aattgctttt tctgttccgg cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc
     3961 gccagcatgt tagctttgcg ctgttcttcc atttttgcga tctgcgcttc agcttttgct
     4021 ttatcgaagg tatcgctggc aatgatgtca tgcattgcgc gacgttcttc cagcggcgga
     4081 cgtttcatct ggtcacgctg gcctttcatg atttcgcgaa tctgctgttt ctgcgcgtcg
     4141 gtcaggttca ggtctttgaa catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc
     4201 atcatcggct tcgcgtcagc cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta
     4261 gccgcgccaa gagccagggt agaggcaaca aacagtgcag ttaatttacg catattctat
     4321 atccttcctt tcagttattt attacggctt tcttaagtag cgtgccgtgt tgacgagatt
     4381 aactttactg actttagcgt caattaatca gagcaacggt aaaacaatga aagtgtaaaa
     4441 aacacttttg cgccaattat ggagaaaaaa agaaaatttg atggagagtg atgagagaat
     4501 attacaacac catgattttg cagagattat gaagaactat accggatgac tggtgataaa
     4561 taaagcaaat aaccaggatt aatctgtatt aatttataag aaggtaactt aatacccgca
     4621 gaaagatttc tgcgggtaag tattagctta ttttttcgag cattaatccc gcgcgtaatc
     4681 ccagcgctac caacggatta gggaataaca catactctac atcatgggtt acggtaaaac
     4741 gttcctcacc gtcctgcgcc agcaacgttc ctttcttaaa cggcataaaa ttcagcgtgt
     4801 cacttgccat atgcatttcg aaggacggcg agtggcgagt aatttgcgaa accacccgat
     4861 aacggagcgg cggtgttctc acgataccga cactctcacc agatagcagc gcagcgattg
     4921 cgctggcagt tactgcaaac tggcgaagat cgttttgccc aaagggcaac gctttgccaa
     4981 gttccagcgt acaggccagc gcgccaaaat gtctggcgct gaaatgggta aacgtaccac
     5041 caggctcctg atggaacacc agcgcctcca gccccgccgc acccagccac gtcaggaatt
     5101 tctcgtccca gggaatgtcg cgttgcggta atacaccgaa ctgcggatgc aaggagccac
     5161 gaattgcggt atgtagatca aggtgccagc gcacagattc tttgccctgg tcataaaaat
     5221 cttccaggca ctgttccagt tcgcgcgccc gacaggtttc cccgctttca gcaaacagct
     5281 gccaacgacc gccaaacatt cggttcatat cgctatggca ataacgtttc ccttgcttca
     5341 gcgcaggagg attcccgagg atcaccagca accgccaccg taacgggatc tcgccgtgag
     5401 atatcgcgcc aagcaatgca tcaagcatct ccacaggtgc cgtctcatta ccgtggatcc
     5461 ccgcagaaat caccagagcg ccttgcggtg gcgttaatgg cgtcagttcc agcacaccat
     5521 cgcccagcca gcgccagcga acgccgttga tttctcgctc ggtgatgaca ggttttttac
     5581 tcgttaaggt cagagcaaga aaattatcca ttgccccctc cctcgcgctg gaacggataa
     5641 accgaaccga gattcagtaa ttggctcaat acatccagtg cttcccgccc ttcgcgcagc
     5701 aattgcgggt cggccagatc ggcagcagta agacgatcgc ggtagtaacg atccacccag
     5761 tcattgagcg cattaaacaa ggtatcgttc atcatcaccg ccggattcac cgcccggcgt
     5821 tcttcttgtg tcaataccac ccgcaaccgc aggcacgccg ggccaccgcc attcgccatg
     5881 ctttcacgta aatcaaagac ttttagttcg ctaatcgggt tgtcagcggc aaggagttca
     5941 ttgagataac cccatactcc ggcgtgttcc cgacactcct gaggcagcac caacatcatg
     6001 gaaccatcat cgcggctcaa cagttggctg ttaaacagat atgtagacac cgcatccgac
     6061 acggaaacct gagttgccgg aacttctatc gccataaaac cgttcacccg cgcacgcagg
     6121 tttgccagta actgtgactg gcgagcgaac gcctgttggt ggcaaaacag cacctggcgg
     6181 ttactcacgg caatcacgtc attatgaaaa acgccctggt cgataacgtc cgggttttgc
     6241 tgggcgaaaa tcacctgttg gggattcacc tgattcagcc ttgccaccgc ctcgctggct
     6301 tcgcgagtct gtcgcgccgg ataacgggaa ggccgggtat cattgccctc ttctcgcccg
     6361 tagacaaaaa gttgcatacc cggttcaccg taatgaccgc cgagacgatt gtggtttgcc
     6421 gccccctcat caccgagcaa ggctacctgc ggcaacgccg aatggacact aaatttctct
     6481 tcgtcgttaa aaatcgcttt taacagcgat tcagtgacgg gcgcttccag cgaacggtga
     6541 aatttattgt tcaggttagc aacggtgaga tgcactttgc catccagcgt atcggcagat
     6601 ggcgcgatcg ttgccgcatt ggctacccac attggcgaag cggaactgac gctggaaagc
     6661 cagtgcggtg cctggcgtac aaccttttcc agtacctgct catcgctacc actgaatccc
     6721 aactgacgca gcaccggaat aaacggacgc tcgtgaggcg ggatcacggc ctgggggaat
     6781 cccgcatcgg caagggtttt cattttcagt aagccctgtt ttgccgccag tcgcgggtta
     6841 gagacctgaa aacggtgacg ggtagaggct ttattaccaa acgacaggcc cgcgtaatga
     6901 tgcgtcagcc ctaccagccc gtcgaaatta acttcccagg cgttcatcgc accacctcat
     6961 cggaaaaatc cagcccgggg ttaagcgttg cgggcaaagt taacgagtcc gactccaggc
     7021 tcgccatagg ccatgcgcag taatctgcgg cataccaggc gctggggcga tggttaccgg
     7081 atgcaccaat gccgccgaat ggcgcggtac tggcagcacc agtaagcggt ttgttccagt
     7141 taacaatccc cgcccgcgcc tccagcaaca gttgatcgaa tttttcccgc tcgggggaaa
     7201 ccagaccgca agagagtccg aagcgagtgt tattcgccat tcgaatcgct tcctcgaaag
     7261 aatcataacg ccagacgcgc aataacggtc cgaacacctc ttcatctggt acgccagcaa
     7321 cgcctgtcat ttcaatgatc cccggcgtca gcaacgatgt ccctgcttgt aataagcgcg
     7381 gcgcaagcag ggttcgtcca cccatcgctt ccagttgctg ccaggcagta aacacctgct
     7441 gtgcggcctg ttcagaaatc agcccgccaa taaacggctg cggttcgtca tcccagttgc
     7501 ccggcgttaa tcgctggctg acggcaacca gacgagcaag aaatgcatcg ccctgcgccc
     7561 cgcttttcag caatacacgg cgggcgcagg tgcagcgttg cccggctgta acaaacgccg
     7621 actgaatggt cagatggaca gccgcgtcga tatccgccac ctcatcgata atcagcgggt
     7681 tattgccgcc catctcaagg gcgagaattt tctccggctg accggagagc tggcgatgca
     7741 gctggtagcc ggtattggcg ctgccggtaa acagcaagcc gtcgagatcc tccagcgcac
     7801 tcagcgcctg acccgtttca cacccgccct gcaccaggtt cagcacgcct ggcggcaagc
     7861 cagcctgctg ccataaacgc attaccgctt cgccactcca cggtgtcagt tcgctgggtt
     7921 taaagataat ggtgttacct gccagcaatg cgggaacgat atgtccgttc ggcaaatgac
     7981 cagggaaatt atacggccca aacaccgcca gcacgccgtg cgggcgatgt cgcaggctcg
     8041 ccgcaccgtc cggcatttca ctacgctgct cgccggtacg aacgtgatac gccttaattg
     8101 atatcgcgat tttattgatc atcgccgtca attcggttgc cgcctcccag cgcggcttgc
     8161 cggtttctcg ggcaatgatt gcggttaatt cgcctttatt cctttccagc aatccggcaa
     8221 agcgttcgac aacggcctga cgttcagcaa atgagagccg cgcccagcgc ggaaacgccg
     8281 cacgggctgc ccgacaagcc tgcccgacct gagcggcatc ggcatcattg ccttgccata
     8341 acacctcgcc cgataccgga ttacgcttca cacgcgatgc accctggccc gttatccagt
     8401 caccgttaat ccataaagtc atgctgtttt ctcctctgcg cacaggcgca ccagacgaac
     8461 gcgatcccct gcatggcatt tgagggcatc cagttgtgcg gcggttaaaa tcaaacgctc
     8521 ggttgccgga tcggtacgca ccagcaccac gcggaaatgg tgataatttt cattggcgac
     8581 caggcaggcc ggaaaatcgc cctgcgcagg ctgcccttct gccacttcca ccagccgact
     8641 tttacggatg gcgcgcacgc ggtcgatgtc acactcaagc gtcggcccac cgtcgaagat
     8701 gtcgatatag ttgcggtagc gaaaaccttc tttctccagc accgcgcggg caggcgcggt
     8761 ttgcggatgt acctgaccga tgacgtcctg ggcttcctgg gataaaaagt gcgtatagat
     8821 cggatgtttc ggcatcagtt ctgcaataaa tgccttttgc ccggtgccgc agagaaaatc
     8881 ggcgcggcta aaatccatcg aaaagaagcg tttaccgagg ctttgccaga acggtgaata
     8941 gccgtgttcg tcaatcaccc cgcgcatttc agcaaccact ttgtcattaa acttgtcgcg
     9001 aaaagccgcc ataaacataa agcgcgattt cgacagcaaa tagccgttgc cctctttgcg
     9061 ccagtccggg tcgagaaaca gcgtgcacag ctcgctgctg ccggtgtgat cgttactgag
     9121 aaacaacgtc ggcaatgcgt tatagacatt cagctctttt gaggcgtgaa ccaacgtgcc
     9181 gacgcgatag ttgtaccagg gatcgttcag cccaactgcc acctcaatgg cgcaaatccc
     9241 cgccacggtg cctgtctcgc tatcttccag tacaaacaca taaccctgct cacttttggg
     9301 cagttcacct tgccaggttt tgattgccct ttcgatacgc gccgaaagcg tggcttcatt
     9361 ggcgggaagc gacgtcaggc cgccgcccgt tttgctggca agctgcatca gcgccgagac
     9421 atctgagcgc tcaacgggac ggatgaccat catgatgaac ctcggctaac aaagtgttcg
     9481 caagcaactg caaagcgatc cagtccggtc gtcacctctt cttcgctgac attgagcgca
     9541 ggcgcaaaac gcaccacgtt gccacccgca atcagtacca tcacgcctgc tttcgccgct
     9601 tcctgagaga tctgtttcgc ttgcccggcg taatcggcat tcagtacaca gccaatcagc
     9661 aaacctaagc cgcgaacttc attgaacaaa ccatagcggt gattaataat attaagacgc
     9721 tcaacaaacc agtcgtgacg ttgcttaacc ccgttaagca tctctggcgt attgatgagc
     9781 tccagcactt tgcctgccac cgccgaggcc agcggattgc cgccataggt ggtgccatga
     9841 gtgccaacgg tcatcacgct ggcgcactct tcggttgcta acaacgcacc gaccgggaaa
     9901 ccgccgccca gagctttggc ggtagttaac agatcaggcg tcacgccgta gtgcatatag
     9961 gcatacagtt ccccggtacg cccgacgccg gtttgtactt catcaaaaat caacagcgca
    10021 ttgtggcgat cacacaattc acgcagacct tgtaaaaatg cgttgctggc tggcaccaca
    10081 ccgccttcac cctggatggg ttcgacaatc accgcacagg tagagtcgtc aatcagcgcg
    10141 ctggcagagt taatatcgtt atatgcagca tgacggatat ctggtggcag tggcgcaaaa
    10201 tcctgtgaat aggctggctg cccacccgca ctaacagtaa acagcgtgcg accatgaaac
    10261 gcatttttaa acgccacgat accgctctta tggctgccgt agcggtcgtg agcgaattta
    10321 cgcgccagtt ttagcgccgc ttcgttggct tccgcaccgg agttacaaaa gaaaacgcga
    10381 tcggcaaacg tggcgtcgat caattttttc gccagtcgca gtaccgactc gttggtgtaa
    10441 ccgttgccgg tatgccagaa cttactcgcc tgttcgttca gcgcctcacg cagttccgga
    10501 tgcgcatggc ccagcgcgtt caccgcaatg ccacccgcga agtcgatata ctctttcccc
    10561 tgctgatccc acaagcgcga accttcgcca cgtaccggta taaacggtgc cggagcgtaa
    10621 acaggtatca tccattcatc aaagttttca cgcgtaattg gctgagacat agcgacctct
    10681 acagtaaata gctcgttatt tatatgttaa taataagtaa tgttttcgct gtaaatgtag
    10741 attgcagggt tcgtgccagc cagtgatgaa agtgcataaa cggcggaggc taactggaaa
    10801 tcaaggagtt ataaccaaac catatgcatt taaagtgcat attaagtgaa tacgtttgcg
    10861 atgtgggtga ataaaaagaa taaaaaacgc aatgttatgc agaagtaaaa tataattctg
    10921 gaattgtgat cattgacgaa atttactgga aattactgcg ccattctgac gcagcgcgca
    10981 ccaaaagcgg gcattttttg cgccatcgtt gacatcatta acaaccatcg atcaaatcac
    11041 ttaacaacag gcggtaagca aagcgaaatt ctgctaccat ccacgcactc tttatctgaa
    11101 taaatggcag cgactatgaa atttgtctct tttaatatca atggcctgcg cgccagaccc
    11161 caccagcttg aagccatcgt cgaaaagcac caaccggatg tgattggcct gcaggagaca
    11221 aaagttcatg acgatatgtt tcccctcgaa gaggtggcga agctcggcta caacgtgttt
    11281 tatcacgggc agaaaggcca ttatggcgtg gcgctgctga ccaaagagac gccgattgcc
    11341 gtgcgtcgcg gctttcccgg tgacgaagaa gaggcgcagc ggcggattat tatggcggaa
    11401 atcccctcgc cgctgggcaa tgtcaccgtg atcaacggtt acttcccgca gggtgaaagc
    11461 cgcgaccatc cgataaaatt cccggcaaag gcgcagtttt atcagaatct gcaaaactac
    11521 ctggaaaccg aactcaagcg tgaaaatccg gtgctgatta tgggcgatat gaatatcagt
    11581 cctggcgacc tggatattgg cattggcgaa gagaaccgta agcgctggct gcgtaccgga
    11641 aaatgttctt tccttccgga ggaacgcgaa tggatggaga gactgatgag ctggggattg
    11701 gttgatacct tccgccatgc gaatccgcaa acagcagatc gtttctcatg gtttgattac
    11761 cgctcaaaag gttttgacga taaccgtggt ctgcgcatcg acctgctgct cgccagccaa
    11821 ccgctggcag aatgttgcgt agaaaccggc atcgactatg aaatccgcag catggaaaaa
    11881 ccgtccgatc acgcccccgt ctgggcgacc ttccgccgct aatttagcag ctctcctggc
    11941 tcaaactggg tcaggagaat taaccttgag aaaaatcaac aaactgtcag taatgatttg
    12001 ttgcctgccg tcctttgtta taccgtcact gcgtttttag ttgtctgacc acttctctat
    12061 tatcaagttt gatataggaa actccacgat gaacgctgag cgtaaatttc tttttgcctg
    12121 tcttattttt gcgctggcca tttacgctat ccacgctttc ggtttattcg atctgctcac
    12181 cgatttaccc cacttacaga cactcattcg ccagagcgga ttgttcggtt atagcctcta
    12241 tattctgtta ttcatcattg ccaccctctt tctgttacca ggaagcatat tggtgatcgc
    12301 gggaggaata gtttttggcc cgttcttagg gacactactc tcattaattg ccgccacgct
    12361 ggcctcctcg tgctcattcc tgctggcgcg ctggatggga cgtgatttac tgctgaaata
    12421 cgttggccat agccatacct tccaggccat tgaaaaaggc attgcgcgta acggtattga
    12481 ttttcttatt ctgacccgct taatcccgtt gtttccttac aatattcaaa attacgctta
    12541 cggattaacc acaatcgcct tctggcctta tacccttatt tcggcactca cgaccctgcc
    12601 cggtattgtt atttataccg tgatggcaag cgatctcgcc aatgaaggca ttacgctgcg
    12661 ctttatttta caactctgtc tggcgggcct ggcgctgttt attctcgtcc agctcgcaaa
    12721 actctacgcc cgacacaaac atgtggatct gtctgcttcg cgccgcaacc cacttactca
    12781 cccaaaaaat gaaggataga acgatgttgc aacattattc agtgtcatgg aaaaaaggac
    12841 tgactgcact ctgcttactg gctgttgctg ggcttagcgg ctgcgatcaa aaagagaatg
    12901 cggcggcaaa agtggaatac gacggacttt cgaacagcca accgctgcgc gtcgatgcca
    12961 ataaccatac ggtaaccatg ctggtgcaaa ttaatggtcg tttcctcacc gacgacactc
    13021 gtcacggtat tgtgtttaaa gatggctcca acggacataa atcgctgttt atgggttatg
    13081 cgaccccgaa agcattttat gaagccctga aagaggcagg tggtacaccg ggcgaaaaca
    13141 tgacgatgga taataaagaa acgactcatg tcacaggcag caaactggat atttcggtca
    13201 actggcaagg ggcggcaaaa acgtattcct tcgatgaagt gattgttgac agtaatggca
    13261 agaaactgga catgcgcttt ggcggtaatt taacggcagc agaagagaag aaaacgggtt
    13321 gcctggtgtg tctggatagc tgcccggtcg gcatcgtcag caatgcaaca tacacttatg
    13381 gtgcggttga aaaacgaggt gaagttaaat tcaaaggcaa tgcctcggtt ctcccggcga
    13441 ataacacgct ggcaacggtt acctttaaaa tcaccgaata aagccaggat aaaggatgat
    13501 gaagatgcaa tcgcgtaaaa tctggtacta ccgtataacc ctcatcatcc tgttgttcgc
    13561 tatgctgctg gcatgggcgc tgcttcccgg cgtccatgag tttatcaatc gcagcgttgc
    13621 ggcgtttgcc gccgtggacc aacagggtat agaacgcttt attcagtctt acggcgcact
    13681 ggcggcagtt gtctcgttct tgttgatgat tttacaggcc attgctgcac cgctacctgc
    13741 gtttttgatc acctttgcca atgcgtcgct gtttggcgcg ttctggggcg gctcgctgtc
    13801 gtggaccagt tcgatggccg gcgcggcact gtgctttttt atcgccagag tgatgggccg
    13861 cgaagtggtg gaaaaattaa ccggcaaaac cgtacttgac agtatggacg gctttttcac
    13921 tcgctacggc aaacacacca tcctggtctg tcggttattg ccttttgtcc ctttcgatcc
    13981 aatcagctat gctgccggtt tgacttcaat acgttttcgc tcgtttttta tcgccaccgg
    14041 gcttggtcag ttaccggcga ctattgttta ttcctgggcg ggcagcatgt taacaggcgg
    14101 taccttctgg tttgtcaccg gactgtttat tctgtttgcc ctgaccgtgg tgatttttat
    14161 ggcgaagaaa atatggcttg agcgccagaa gaggaatgcc tgatgggttt accgccgctt
    14221 agcaaaattc ctttcatttt acgtccgcag gcgtggctgc atcgtcgcca ttacggcgag
    14281 gtgctaagcc ccattcgctg gtgggggcgg atcccgttta tcttttatct ggtgtcgatg
    14341 tttgttggct ggctggagcg caaacgctca ccgctcgatc cggtagtacg atcgcttgtc
    14401 agcgcgcgca ttgcgcaaat gtgcctgtgt gagttttgtg tggatatcac cagtatgaaa
    14461 gtcgccgagc gcaccggcag cagcgataaa ctgctggcag tggctgactg gcggcaaagc
    14521 ccgctcttta gcgatgaaga acggctggcg ctggagtacg ccgaagccgc aagcgtaacg
    14581 ccgccaacgg tcgatgatgc cctgcgtacc cgactggctg cgcattttga cgctcaggag
    14641 ctcaccgaac tgacggcatt gatcggcctg caaaatctgt cagcccgttt taattctgcc
    14701 atggacattc ccgctcaggg gctgtgccgt attccggaaa aacgttctta aggagagatg
    14761 atgcgccatt gtgggtggtt gctgggattg ttatcgctgt tttctctggc aacacatgcc
    14821 agtgactggc aagaaattaa aaatgaggcc aaagggcaaa ctgtctggtt taacgcctgg
    14881 ggcggcgata ccgcaattaa ccgctatctc gactgggtta gcggcgagat gaaaacccat
    14941 tacgctataa acctgaagat tgttcgtctg gcggatgccg cagacgcggt gaagcgcatt
    15001 cagaccgaag ctgctgccgg acgtaaaacg ggcggctcgg tggatctgct ctgggtgaac
    15061 ggcgaaaact tccgcacctt aaaagaggcc aatttactgc aaaccgactg ggcagagact
    15121 ctgcccaact ggcgctatgt cgacacacag ctgccggtgc gggaagattt ttcagtgcct
    15181 acagaagggg ctgaatcgcc ctgggggggc gcacaactga catttatcgc ccgccgcgat
    15241 gttacgccac agccaccaca aacgccgcaa gccttactgg agtttgctaa agccaatccc
    15301 ggcacggtta cctatccgcg cccaccggac tttaccggca cggcgtttct tgaacagttg
    15361 ctgattatgc tgacgcccga tcccgccgca ttaaatgaag cgccgaacga tgcgactttc
    15421 gcccgtgtca ctgctccctt gtggcaatat cttgatgcgc tgcatccgta tttgtggcgc
    15481 gaaggaaagg atttcccgcc ttcgcccgcg cggatggatg ctctgctgaa agccggaaca
    15541 tttcgcctgt cgctgacctt taaccccgcg catgcgcagc aaaaaatcgc cagcggcgat
    15601 ttgcctgcaa gcagttacag ttttggcttt cgcgagggga tgattggcaa cgtgcatttc
    15661 gtcaccattc ctgccaacgc gaatgccagt gctgcggcga aggtagttgc caatttcttg
    15721 ctctcacccg atgcgcaatt gcgtaaagca gatcccgctg tctggggcga tccttctgtt
    15781 ctcgatccgc aaaaactgcc tgatgggcag cgcgaaacat tgcaatcaag aatgccgcaa
    15841 gatctgccgc cggtactggc tgaaccgcac gcaggttggg taaatgcgct ggaacaagaa
    15901 tggctacacc gttacggtac gcattaatct ttttgctgtg ggcgatggtg gcggtgattt
    15961 atgcaccgct gatcccggca gctctcacgc tgatatcgcc tgctttgtcg ttgacacact
    16021 ggcaggcgtt atttgccgat ccacagttac cgcaggcatt actggcaacg ctggtgtcga
    16081 caaccatcgc ggcggtcggg gcgttgttga ttgccctact ggtgattgtg gcgctgtggc
    16141 ctgggccgaa atggcagcgt atgtgcaccc gtctgccgtg gctgctcgcc attccccatg
    16201 tggcttttgc caccagcgcc cttctgctct ttgctgacgg agggctgctt tatgactatt
    16261 tcccgtattt cactccgcca atggacaaat tgggtattgg gctgggcttc acgctggcgg
    16321 tgaaagaaag cgcatttctg ctgtggatct tagcagcggt attgagtgaa aaacggcttt
    16381 tacagcaagt catcgtgctg gattcgctgg gctacagccg ctggcaatgc ctgaactggc
    16441 tgctgttgcc ctccgtcgcg cctgcgctgg caatggcgat gctggcgatt gttgcctggt
    16501 cgctgtcggt cgtagatgtg gcgattattc ttgggccagg taatccaccg acgctggcag
    16561 taattagctg gcagtggtta actcagggcg acgccgatca acaaacgaaa ggcgcactcg
    16621 ccagcctgct gctgatgttg ttactcgccg cctacgtttt gctgggctat ctgctatggt
    16681 gtacctggcg gcgtactatt ccccgcgtag atggcgttcg taagcctgcc acgcctttat
    16741 tgccaggcat tacgctggcg agttttttac ccttgaccag tgtgctgtgt gtggttctgc
    16801 tggcgatcct cgcggatcag tcgacgatca atagtgaagc gctcatcaac agcctgacaa
    16861 tggggctggt ggcgacattc atcgctttgc tcctgttact gctgtggctg gaatgggggc
    16921 cacagcgtcg ccagttgtgg ctatggttgc cgattttatt accggcactg ccgctggtgg
    16981 cgggccagta cacgctggcg ctatggctga acatggatgg aagctggacg gcggtggtct
    17041 gggggcatct gctgtgggta atgccgtgga tgctgtttat cctgcaaccc gcctggcagc
    17101 gcattgattc acggttaatt ttgattgcac aaacactggg ctggtcgcgg gccaaaatct
    17161 tcttttacgt gaaatgtccg cttatgctac gccctacgct gattgccttc gcggtgggat
    17221 tttcagtcag tattgcgcag tatatgccga cgctgtggct gggcgcgggg cgttttccga
    17281 cgctcaccac tgaggcggtg gcattaagca gcggcggcag caacggtatt ctcgccgccc
    17341 tggctttatg gcaactgcta ttaccgctta ttatttttgc cctgaccgcg ttagtcgcaa
    17401 aatgggtagg ttatgtcaga caaggactcc gctaatgctc tgcgtgaaaa atgtttcgct
    17461 acgtttacca gaaagttgct tgctgacaaa cgttaacttt acggtggata aaggtgacat
    17521 tgtcacgtta atggggccgt ctggctgtgg aaaatccact ctgttttcat ggatgattgg
    17581 tgcactggcc ggacagtttt cttgtacagg tgagctatgg ctcaatgagc aacggattga
    17641 catgctaccc accgcacagc gtcagattgg cattcttttt caggatgcac tgttatttga
    17701 ccagttcagt gtcgggcaaa atttactgct ggcgctaccg gcgacactta aggggaatac
    17761 ccgacgtaat gccgtgaatg atgcacttga gcgttcaggc cttgacggag ctttccatca
    17821 ggatcctgct actttgtctg gcggtcagcg agcgcgcgtt gctctgcttc gcgcccttct
    17881 cgctcagcca aaagcgttgc tactggatga gccattcagc cgtcttgatg tggctctgcg
    17941 cgataatttt cgccagtggg tgttcagcga agttcgcgaa ctggcgatcc ccgtcgttca
    18001 ggtaacgcac gatctccagg atgttcctgc tgatagttct gttctggata tggcgcagtg
    18061 gtcagaaaat tacaacaaac tgcgataacg caaagttttt ctcaatgcgt cagttcagaa
    18121 tggcgcactc aaaactacaa tgtcgggatt ttcgatgaaa cgtgtttctc aaatgaccgc
    18181 gctggcaatg gctttagggc tggcttgcgc ttcttcgtgg gccgctgaac tggcgaagcc
    18241 tcttacactt gaccagcttc aacaacaaaa tggcaaagcg atagatactc gctccagcgc
    18301 gttttataac ggctggccac aaaccttaaa tggcccttct ggtcatgaac ctgccgcctt
    18361 aaacctctct gctagctggc ttgacaaaat gagcaccgaa cagctcaacg cgtggatcaa
    18421 gcaacataac ctgaaagccg atgccccggt ggcgctgtac ggtaatggca aagatgtcga
    18481 cgccgtcaaa acgcgactgc aaaaagcagg ttttacgcat atctccattc tgagtgacgc
    18541 gctaagcgaa ccttcccgtc tacaaaaact gccgcacttt gagcagctgg tttatccgca
    18601 atggctgcac gacctgcaac aaggtaaaga ggttacggcg aaacctgccg gtgactggaa
    18661 agtcattgaa gcggccaggg gcgctcctaa gctttacctt atcagccata ttcccggtgc
    18721 tgactacatc gataccaacg aagtggaaag cgaaccgctg tggaacaaag tttctgatga
    18781 acaactaaaa gcgatgctgg caaaacacgg cattcgccat gacaccacgg tcattctgta
    18841 cgggcgtgac gtatacgctg cagcgcgtgt ggcgcagatt atgctttatg ccggcgtgaa
    18901 agatgtgcgc ctgctggatg gcggctggca aacctggtcc gacgcgggtc tgcccgttga
    18961 gcgcggtacg ccaccgaaag tgaaagcgga accggatttc ggcgtgaaga tcccggcaca
    19021 accgcagttg atgctcgata tggaacaggc ccgtggactg ctgcatcgcc aggatgcatc
    19081 gctggtgagc attcgttcgt ggccagaatt tatcggtacg accagcggtt acagctatat
    19141 taaaccaaaa ggtgaaatag ccggagcacg ttggggacac gctggtagcg actcgacgca
    19201 tatggaagat ttccataatc cggatggcac catgcgtagc gccgatgata ttaccgctat
    19261 gtggaaagca tggaatatca aaccagatca gcaagtttca ttctactgcg gcaccggctg
    19321 gcgcgcgtcc gaaaccttta tgtacgcacg agcaatgggc tggaataacg tctccgttta
    19381 cgacggcggc tggtacgaat ggagcagcga tccaaaaaat ccggtagcaa ccggtgaacg
    19441 cggcccggac agcagcaaat aacattgcaa ttactgacgc tggagcgact tcagcgtcag
    19501 atacccactc cacacccgcg taaaggtcgt catccagcac aacgcaccga agatccacgc
    19561 aaaccacgca aaccatgcag ggaataaaca tcccagcaca aacagtaaga tcgtttctgt
    19621 gccttcggtt aatcccccca gataataaaa cgatttatgc gcataaccgg gattatcaat
    19681 ctgatatttc gccgccagcg cagcaaaagc gagaaaactg ctacccgtgc cgataaacgc
    19741 aaacagcagc cagccgcctg ccagggcgtt ttgctgcggt gcggcgagga taaacccaaa
    19801 aggtaccagc gcgtaaaaaa gaaaatcgag agaaatatca agaaagccgc ccgcgtcggt
    19861 aagccctctg cgccgcgcca gcgcaccgtc cagaccatca agcagcctgt tcaacaaaat
    19921 gacgacaagc gccgccagat accagcccag cgccagaaaa ggcagtgcca gcacaccgat
    19981 ggcaaatccc actaatgtta aaccatctgg cgtaatgccc ggtttatcaa gaacccgcac
    20041 acactgatgc aacaacggtt taatccgggg atgaagatgg cggtctagca caggcactcc
    20101 ttaaatataa agcctttctg attgagcaac agtgcggata ttatggcatt tttcgcttat
    20161 ctgcccgtgt gtaatttatg aaaatgattg aagttgttgc cgccatcatt gaacgtgatg
    20221 gcaaaatttt actcgcgcaa cgccccgccc agagcgatca ggcgggatta tgggagtttg
    20281 ccggtggtaa agtcgagccg gatgaaagcc agcagcaggc gctggtgcgt gagttaaacg
    20341 aagaattggg catcgaagca actgtgggtg agtatgttgc cagccatcag cgagaagttt
    20401 cgggccggat tatccatctt catgcctggc acgtacccga cttccacggg acgttacagg
    20461 cacatgaaca tcaggcgctg gtctggtgct cacctgaaga ggcgctgcaa tatccgctgg
    20521 ctcctgctga cattccatta ttagaggcgt ttatggcttt acgcgccgcc agaccagcgg
    20581 attagtgcta agggttttgt catcccgctg gcattgcagc agtattcctt cggctttaat
    20641 taccgcccct tcagaataat ttttatcctg ataaacgcag cactgagtac agggctgcgc
    20701 tgactgcccg cctgaactga ccacttctgg cggtacgttt acctccacgt ccggaccata
    20761 atgcgggtta gccagtgcaa ttaatggaaa tgctaatact acggcgaaca atgctcgact
    20821 cacagggaac tccttaacgt tattgtctct gctactgata acggtagccg ggtggcaaaa
    20881 ctttagcgtc tgagttatcg catttggtta tgagattact ctcgttatta atttgctttc
    20941 ctgggtcatt tttttcttgc ttaccgtcac attcttgatg gtatagtcga aaactgcaaa
    21001 agcacatgac ataaacaaca taagcacaat cgtattaata tataagggtt ttatatctat
    21061 ggatcagaca tattctctgg agtcattcct caaccatgtc caaaagcgcg acccgaatca
    21121 aaccgagttc gcgcaagccg ttcgtgaagt aatgaccaca ctctggcctt ttcttgaaca
    21181 aaatccaaaa tatcgccaga tgtcattact ggagcgtctg gttgaaccgg agcgcgtgat
    21241 ccagtttcgc gtggtatggg ttgatgatcg caatcaggta caggtcaacc gtgcatggcg
    21301 tgtgcagttc agctctgcca tcggcccgta caaaggcggt atgcgcttcc atccgtcagt
    21361 taacctttcc attcttaaat tcctcggctt cgaacaaacc ttcaaaaatg ccctgactac
    21421 tctgccgatg ggcggtggta aaggcggcag cgatttcgac ccgaaaggga aaagcgaagg
    21481 cgaagtgatg cgtttctgcc aggcgctgat gactgaactg tatcgccacc tgggcgcgga
    21541 taccgacgtt ccggcaggtg atatcggggt tggtggtcgt gaagtcggct ttatggcggg
    21601 gatgatgaaa aagctctcca acaataccgc ctgcgtcttc accggtaagg gcctttcatt
    21661 tggcggcagt cttattcgcc cggaagctac cggctacggt ctggtttatt tcacagaagc
    21721 aatgctaaaa cgccacggta tgggttttga aggcatgcgc gtttccgttt ctggctccgg
    21781 caacgtcgcc cagtacgcta tcgaaaaagc gatggaattt ggtgctcgtg tgatcactgc
    21841 gtcagactcc agcggcactg tagttgatga aagcggattc acgaaagaga aactggcacg
    21901 tcttatcgaa atcaaatcca gccgcgatgg tcgagtggca gattacgcca aagaatttgg
    21961 tctggtctat ctcgaaggcc aacagccgtg gtctgtaccg gttgatatcg ccctgccttg
    22021 cgctacccag aacgaactgg atgttgacgc cgcgcatcag cttatcgcta atggcgttaa
    22081 agccgtcgcc gaaggggcaa atatgccgac caccatcgaa gcgactgaac tgttccagca
    22141 ggcaggcgta ctatttgcac cgggtaaagc ggctaatgct ggtggcgtcg ctacatcggg
    22201 cctggaaatg gcacaaaacg ctgcgcgcct gggctggaaa gccgagaaag ttgacgcacg
    22261 tttgcatcac atcatgctgg atatccacca tgcctgtgtt gagcatggtg gtgaaggtga
    22321 gcaaaccaac tacgtgcagg gcgcgaacat tgccggtttt gtgaaggttg ccgatgcgat
    22381 gctggcgcag ggtgtgattt aagttgtaaa tgcctgatgg cgctacgctt atcaggccta
    22441 caaatgggca caattcattg cagttacgct ctaatgtagg ccgggcaagc gcagcgcccc
    22501 cggcaaaatt tcaggcgttt atgagtattt aacggatgat gctccccagg gaacatttct
    22561 tatgggccaa cggcatttct tactgtagtg ctcccaaaac tgcttgtcgt aacgataaca
    22621 agcttcaagt tcagcatccg ttaactttct gcgatagcag cagatatgcc agtaaagaaa
    22681 tcccattaga ctattttttt gataatcttc ttcgctttcg aacaactcgt gcgcctttcg
    22741 agaagcaagc attatataat gccaggccag ttcttcttca attgtcccgt tttgaaaagc
    22801 tgtgcttgat atcgagatca tccatgataa ttccgccgcc catattagct tcgccgagga
    22861 tttaccggag ctatgattag cgcaatcaga gatatagtct gagggaaaaa cagcaaattt
    22921 attcaacaag gcgataacct gctctggggc ttcctccatg tttgctttaa aggtattggc
    22981 tccatggtcg ccagaaagaa aatgctccat taaggcacaa taactttcgc tatcttcgat
    23041 accccattgt tcctctaaag actcgcgtct tttacttatg atatcgatcg agtcaaaagg
    23101 aagcacatga tattggaagg tatctttgcc aggttcaggc tttcgcggcc agaactccag
    23161 cgtttcagac cattgcttat gatagaatcg ataaggtgcg atcaattgta gcgcctgtaa
    23221 tttctcgata ctgagcggct caataccttt agcctgataa taatgcagct gttctttttt
    23281 tgctttaaaa ccggcccgaa caataagccc catcataatt aatagataaa gaaaagagca
    23341 tcccgcggta atcaggcctc tttcattcaa accgttggat gttatcgctg cgaatacaaa
    23401 cattacagcg acaacacatg ttaaataaaa cccccactta caaagcagca tggtcttatt
    23461 ttctttaatc atccgttcaa aattactatt aaatatttcc cagccattaa aagaatactt
    23521 ctcgctccca ggatggttt
//
LOCUS       DBFIFM010000061        23511 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.061, whole genome shotgun sequence.
ACCESSION   DBFIFM010000061 DBFIFM010000000
VERSION     DBFIFM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.061"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..516)
                     /locus_tag="N7C74_002045"
     CDS             complement(<1..516)
                     /locus_tag="N7C74_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2431917.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGH"
     gene            complement(573..1778)
                     /gene="rhmD"
                     /locus_tag="N7C74_002046"
     CDS             complement(573..1778)
                     /gene="rhmD"
                     /locus_tag="N7C74_002046"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HGU2431918.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQLRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            complement(1793..2575)
                     /locus_tag="N7C74_002047"
     CDS             complement(1793..2575)
                     /locus_tag="N7C74_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HGU2431919.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCNAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTKLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPATVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(2795..3997)
                     /locus_tag="N7C74_002048"
     CDS             complement(2795..3997)
                     /locus_tag="N7C74_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HGU2431920.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTLALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(4097..4639)
                     /locus_tag="N7C74_002049"
     CDS             complement(4097..4639)
                     /locus_tag="N7C74_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HGU2431921.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            4918..5343
                     /gene="nudI"
                     /locus_tag="N7C74_002050"
     CDS             4918..5343
                     /gene="nudI"
                     /locus_tag="N7C74_002050"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HGU2431922.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLAHYDLNVATRKTLRLKGLL"
     gene            complement(5382..5984)
                     /gene="ais"
                     /locus_tag="N7C74_002051"
     CDS             complement(5382..5984)
                     /gene="ais"
                     /locus_tag="N7C74_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HGU2431923.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLGRLAQQHPVVVLFRHAEHCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            6292..7431
                     /gene="arnB"
                     /locus_tag="N7C74_002052"
     CDS             6292..7431
                     /gene="arnB"
                     /locus_tag="N7C74_002052"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HGU2431924.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNHALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLQGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADINAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENIARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPSLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            7435..8403
                     /gene="arnC"
                     /locus_tag="N7C74_002053"
     CDS             7435..8403
                     /gene="arnC"
                     /locus_tag="N7C74_002053"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HGU2431925.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHILVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREYGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            8403..10385
                     /gene="arnA"
                     /locus_tag="N7C74_002054"
     CDS             8403..10385
                     /gene="arnA"
                     /locus_tag="N7C74_002054"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HGU2431926.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHHCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            10382..11272
                     /gene="arnD"
                     /locus_tag="N7C74_002055"
     CDS             10382..11272
                     /gene="arnD"
                     /locus_tag="N7C74_002055"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HGU2431927.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKYHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            11272..12924
                     /gene="arnT"
                     /locus_tag="N7C74_002056"
     CDS             11272..12924
                     /gene="arnT"
                     /locus_tag="N7C74_002056"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HGU2431928.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLTIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            12921..13256
                     /gene="arnE"
                     /locus_tag="N7C74_002057"
     CDS             12921..13256
                     /gene="arnE"
                     /locus_tag="N7C74_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HGU2431929.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAISKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            13256..13642
                     /gene="arnF"
                     /locus_tag="N7C74_002058"
     CDS             13256..13642
                     /gene="arnF"
                     /locus_tag="N7C74_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HGU2431930.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(13636..13902)
                     /gene="pmrD"
                     /locus_tag="N7C74_002059"
     CDS             complement(13636..13902)
                     /gene="pmrD"
                     /locus_tag="N7C74_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HGU2431931.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKVAGKTQ"
     gene            complement(14012..15367)
                     /gene="menE"
                     /locus_tag="N7C74_002060"
     CDS             complement(14012..15367)
                     /gene="menE"
                     /locus_tag="N7C74_002060"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HGU2431932.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AVQGVVEDSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHVATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQTEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(15364..16326)
                     /gene="menC"
                     /locus_tag="N7C74_002061"
     CDS             complement(15364..16326)
                     /gene="menC"
                     /locus_tag="N7C74_002061"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HGU2431933.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRTAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(16326..17183)
                     /gene="menB"
                     /locus_tag="N7C74_002062"
     CDS             complement(16326..17183)
                     /gene="menB"
                     /locus_tag="N7C74_002062"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HGU2431934.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(17198..17956)
                     /gene="menH"
                     /locus_tag="N7C74_002063"
     CDS             complement(17198..17956)
                     /gene="menH"
                     /locus_tag="N7C74_002063"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HGU2431935.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCLEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILSFWLVGYSLGGRVAMMAACQGLAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRSEPLTAVFADWYQQPVFASLNDDQ
                     RRELVVLRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(17953..19623)
                     /gene="menD"
                     /locus_tag="N7C74_002064"
     CDS             complement(17953..19623)
                     /gene="menD"
                     /locus_tag="N7C74_002064"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HGU2431936.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIACRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETVLADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(19712..21007)
                     /gene="menF"
                     /locus_tag="N7C74_002065"
     CDS             complement(19712..21007)
                     /gene="menF"
                     /locus_tag="N7C74_002065"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HGU2431937.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIAHHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(21086..21391)
                     /gene="elaB"
                     /locus_tag="N7C74_002066"
     CDS             complement(21086..21391)
                     /gene="elaB"
                     /locus_tag="N7C74_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HGU2431938.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(21446..21907)
                     /locus_tag="N7C74_002067"
     CDS             complement(21446..21907)
                     /locus_tag="N7C74_002067"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU2431939.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEIYEEDGIPHIGMAREVIQA"
     gene            21972..22889
                     /gene="rbn"
                     /locus_tag="N7C74_002068"
     CDS             21972..22889
                     /gene="rbn"
                     /locus_tag="N7C74_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HGU2431940.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPSPLFQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            23077..>23511
                     /locus_tag="N7C74_002069"
     CDS             23077..>23511
                     /locus_tag="N7C74_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HGU2431941.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNDTLSIIKLIKNACLKLYNLAHTATK
                     HPLKSHDSDNLLFKKLFSPSKLMAIIGEDIPLISEKQSLSKVL"
BASE COUNT         5713 a         6271 c         5848 g         5679 t
ORIGIN      
        1 atgccccata aatccatgca tctccagcag cgcgccggaa agcggtgatc ccagtgttaa
       61 cgccagcggt gcgcccatat agaacagccc cataatgctg gcgcgattac gctgcggaaa
      121 ccattgcgag gtgagataaa tcataccagg gaaaaatccg gcctccgcag caccaagcag
      181 agtgcgaact atcagaaatt tcgcttcagt atcggcccat gccatggctg ccgaaagaaa
      241 tccccacagc agtgttgtcg taccaatcca ggttctggcc cccagtttgc gcatcaaaag
      301 attcgccgga acacccagaa acgcatatac cacaaagaaa atgcctgctc ccagcgcata
      361 agcttcatta ctcaacccgg tatcaatctg gtaggtctgt ttggcaaaac cgatattcga
      421 acggtcgaga aacgccagca catacagcgc caacataaac ggaattaaac gcacacggtt
      481 tttcttcacc acggcgtcaa gcaaagcggt gctcataaaa agctccttag aatatgtggg
      541 cgttaccgtt gtcacagcaa cgcccggagt gattagtggc tgtaggggcg tttcaaattg
      601 cagtcacgat tgagttcgac gccaaaaccg ggtttatcaa gcactgattt atgaatacga
      661 ccattcaccg gaaccggctc attgagcaga atcgggtcaa actgcggacg catcgttgaa
      721 caatccggac tagtcatcag gaattcgctg aatggcgtat tagtgaaggt gatcaccgca
      781 tggtgggagt aaacagacga accgtgcggc accaccagtt gccccctgga tttggcgatt
      841 gcggcaattt ccaccagcgt ggttaagcca ccgcaccagc caacatccgg ctgcataatg
      901 tcgataccgg tttctgaaag cgtacgaaaa gattgcagtg tgccgtggtg ctcaccgctg
      961 gtgaccatca ttccgactgg cgcgttgcgt ttcagttcgc gataaccttc atactgctgt
     1021 ggcggcaggc actcttcgat ccatttcagg ttatagggcg cgcaagcgtg ggccagtttg
     1081 gtcgcatagt tcacgtcctg actcatccag cagtcgagca ttaaccagaa atcctcaccg
     1141 catttttcac gcatatccgc gaccatagcg gcatctttgc ggatccccgc atcgccatca
     1201 tgtggccccc agtgcgtcgg cattttgcca ccgataaagc ccatctcttt tgccagatcc
     1261 ggacgcgcac ctgtggcgta gaactgaatc tcatcacgaa cagcgccgcc taaaagttta
     1321 taaaccggaa gcccgaccac tttgccgaac agatcccaca gagccagatc gacacaggaa
     1381 atcgtgttca tcaccaggcc accagagccg gagtaataca gggtggcatt gagcatttga
     1441 tcatggatca gtttgatatc actgacacat ttaccctcaa tgaaacggtt aaggtgtttt
     1501 tcgacgataa aacagcccat ttcaccggct gtcgaaacag cgaatccggt ctggccgttt
     1561 tctgcttcga cttcaacaac caacgtgcca agaacgttaa tgccaaatga ctggcgtaac
     1621 tgctcgtaat cgcggtattt actcatcggg gtggcaatat gatcgtctat ccagtgattc
     1681 gccccctggt cgtgataatc accgccgcca gcgccttttt ctgctgtcgc accgccagta
     1741 aaccaggcgc gaacctgttt aatttttggt agggtcatga tgttctccat tgttatgagg
     1801 cttgtaagtc aaagggactt ttccatccca acagacgtga aatatccctg gcgcaggcaa
     1861 tggccttgcc cgccagataa tcacggtatt cttcattgat ttgtaagcgg gtaccgacca
     1921 ccgagatcgc agcggtaagc tcgttattgg cgttaaacac cggcgcagcg acacaacgga
     1981 catcggcgta atcttcgccg ttgtcatagc tccagccctg acggcgaata cgcgccagtt
     2041 cttcgtgaag ttgctgtgga tgagtaatcg ttgtgggtgt cgcctgctcc cagaccagcc
     2101 cttcgataat actttgctgt acagttgcag gttgccaggc aagcaggcat ttacctattc
     2161 cggagcgata aagcgaaagg ctttttcctt catgtgaacg cacgctgata gtggctgatg
     2221 actccacttt caaaatgtaa taagcgctgc cgttatcaat aatccccagg tgacataaca
     2281 gcccggtggt atccatcagt tgcgtcaaac gcggtcgagc cagttcccgg agatccatct
     2341 tgcttaacgc atgaccggaa agctccacca gcttggtcca caaacaaaaa ttttcctggt
     2401 gatcaaggct aagaaaacgc tgacgcctga gttcattaag cagcaaatag gccgtgcttt
     2461 tagggatccc caatgtgtca atgatcgtcg cagcattaca ggggccaatg cgggcaatca
     2521 aattgagaat atcaatagcc cgtgtcaggg cgggaacttt gcttgattcc aacatactgg
     2581 actccagtct tagatactgg aatcagtctc gttgctcagg gggagtaaaa ttgtgaagcg
     2641 catcaaatcc cgctcctcgt ccaacggact ggagaaatta cgcacaaatt tgtgaatcag
     2701 cgcacggatt ttttcttatt gagtacaaca ggttggacaa gttttgccgg agggggaatg
     2761 tgccggtgca gtaaaccggc acagagtggc ggaatcagac ggataaggtc atggactcaa
     2821 caacctcaat ccagccatgc tcactggcaa tgtcctggcc gtttaaccaa cggcgtagca
     2881 tattcagtgc catcattgcg cacacttcct gacggatagc caggctatag cgtgtagtgc
     2941 tgaaacgcac acgcagagct aaagtgccat ctggcgtggc tagcgcaaag ttgagatgct
     3001 cgttctcgaa acccgaaaca gccagtgcca gcccggcaaa atggttggct cgccgttctg
     3061 taatccagtg cgcagtttgc gccagggttt cttcctgtga aggaaccact tcacacgcca
     3121 gcagtggagc acctgcgcga gaaagttgca aagccaataa accaccggtg aattgctcgc
     3181 tcaacgtcag gctgaactgg cggttttgca attcgcgact gatctgcgcg ggcagtcctt
     3241 cggtgccttc gaaaatcacg ctctgtccgg caacacgttt aacgtccagc cacagttttt
     3301 ccatcgcctg ttgctcgctt gccggtccgg tgagtttcag ttcgatgatc ggcattgagg
     3361 agcgatagcc cattgttacg cccggcggca gttgtagagt gtccaggctt tgtgccagat
     3421 cgctttccga acgaccaaag gtagttaaac gcagacaaac cggcggctgt ggtaaagaaa
     3481 aacgctcgcg cagacgcggc aggatttcgt gctcgaccat caccttaaat tctgacggta
     3541 cgccgggagt aaagaacatc aggcaacgat taagctgcac ggcaaaacca caggcggtgc
     3601 ctaccgggtt attgataaat tcagcactgg caggcagctc cgcttgttta cggttgctcg
     3661 gtgccattac tcgtccacgt tcgtggaaat agcgttccat ctctttgagc caggcttcat
     3721 gcagcaccag gccttcacct tttgctgtcg cagcggcgag tgcgcttaaa tcatcgctgg
     3781 tcggtcccag cccgccgtta acgatcagca catcggcgtg ctgactacgt tcgcgaagaa
     3841 tggtgactaa gtcatcaagg ttatccccca ccgtattgcg gcgagataat ggcaacccct
     3901 gatgaaagaa aaaatcggcc agccaggcag cgttagtgtc aacgatttgc ccgtgtaaca
     3961 cttcatcccc ggtggatagc atttccactt ttaacatggt tatctcctgc ttttgttatc
     4021 gaatcactat aacgcaaagg gagataaggc gtagtaagaa actgaatggc gcgacagaat
     4081 gccgcgccgg aagggatcag aaactggcgt taaccccaac atacgggccg tcagccacgg
     4141 cgttgtcgcg gttaccgtct ttacccgaca gattcaggta gcgataaccc gcctcaatac
     4201 tgactggacg cataatggtg taacgcgcgc cagcattcgc ttcctcataa cttttaatac
     4261 cgctggagag tgaatccgga gagtagtaat actcgccaaa caaacggaag ctgttgccaa
     4321 ttttccactg caaaccacct cctaccgctg cggcatagcc ttcatcgccg taattcgggt
     4381 tggtgtaaac gccttttccg ccaacggtcg ccattaacgg cccgagaggc agattcaacc
     4441 ccagccccac gcccgcgacg tcaccgtcgt cgtcgttatg tgtccagtta ccgcttaaag
     4501 ccaggcccgt cgattcagtc ccaaaaccga caccaatatt ggtgtattct ttacccgcct
     4561 ggccgctgat gctcattgca ttgaccgatg cagaaaccaa cagcattccg gccagacctg
     4621 ctagcgcaat ttttttcatt atcccctttc ccacaagcaa ttaatcaaaa ttcccaaagc
     4681 tgcgggattg tactgcctgg cgcagaggat tcaatgagcg aaaaaaggga tctagtcact
     4741 tattgtaact aaatacttct taaataatta aacgccgaaa aaataaacac agtttaccga
     4801 tacacacaaa aataataata agtgcagtta tcatcgcaaa agagattact gtcactttca
     4861 catatttctg agttaccgta ttctcatggt ttctttttat ttataaggaa tacccctgtg
     4921 cgacaacgga ctattgtatg ccctttgatt caaaatgatg gtgcttattt gctgtgtaaa
     4981 atggccgacg atcgcggcgt tttccccggt caatgggcgc tttcgggtgg cggcgtggag
     5041 cccggcgaac gaattgaaga ggctctacgc cgcgaaattc gcgaagaact gggagaacag
     5101 ctgcttttga cagaaatcac gccctggacc ttcagcgatg atattcgcac caagacgtat
     5161 gcagatggtc gcaaggaaga aatttatatg atttacctga tttttgattg cgtttctgcc
     5221 aaccgagaag tgaaaataaa cgaagagttt caggactacg cgtgggtaaa acctgaagat
     5281 ctggcgcact atgatttgaa tgtcgccacc cgaaaaacgt tacgtttgaa aggtcttctg
     5341 taacaatgat gacagccatt actggctgtc agattgaaaa tttagtgatt aacgaattcc
     5401 ccatccagat aaactttgcc tttttccaca tgcatgacta aaccatccag ataatcaggt
     5461 ttaaatgtcg catcacgctt atctttagca atatatgtca ggcaatgatt atgggtgaaa
     5521 ataacgatat ttttatcagg cgctttgctt tgtaagtcct taatcgcact ataaatctca
     5581 ttaccgcact gaagaagtcg tttatctacc gtcaattttt tacccgctga aaaccaggtc
     5641 gcggactgaa tggtccggac ggtattactg gaataaagat cgaaatcagg gatatcagca
     5701 ctaaaagcgt tgcccagttc acgggcatcc tgggtacctt taaccgtaat acctgtttta
     5761 tctgacaagc attgattggt tgaacgatcg caatgttcag catggcgaaa caaaacgaca
     5821 accgggtgct gctgtgccag tctgcccagc gttttattgt cgatacgtgg caaaccattg
     5881 ctactccagg cggcatgagt acccagtccg gcaattgcag cgagcgccag taaaatgatg
     5941 aaatattttt ttgacttcaa tgatgagcgg caaaaagcta acacgtaaac tccacctata
     6001 gacaagcgca accagacaat taccgtgaaa ttgagcgaca tttctggcga taattagcag
     6061 ttggtgtaat attaaaaatc ctacgatgct ggcaaaatgc ctcaaaattt tgccaaatgc
     6121 aaagcctaaa taagaaaaaa tataaaaatt tcaatattta cgtctaatat tagtttctta
     6181 aggttaagtt aatattctat ccttaaaatt tcgctctaaa tggcaaaata tacacaacac
     6241 tctttatagc aaatataagt ggacaggtat tcaatggcgg aaggaaaagc aatgtcagaa
     6301 tttttgcctt tttcgagacc agcaatgggc gtggaggaac tcgctgcagt taaagaggtt
     6361 ctcgaatccg gttggatcac aaccggtccg aagaatcacg cgcttgaaca agccttttgc
     6421 cagttgacgg gaaatcagca tgccatcgcg gtcagttcag ccaccgccgg aatgcatatc
     6481 acgctaatgg cgttggaaat tggcaagggc gatgaagtga ttacgccttc cctgacctgg
     6541 gtttcaaccc tcaatatgat ttccttgcag ggtgcaacgc cggtaatggt ggatgtcgat
     6601 cgcgatacgc tgatggtcac gcctgaagcg atcgaatcag ccattacgcc acgcactaaa
     6661 gccatcattc cggtgcatta tgccggtgcg ccagcagata ttaacgccat tcgcgccatt
     6721 ggcgaacgtt acggcatcgc agttatcgaa gatgctgccc atgccgtagg tacgtattac
     6781 aaagggcgac atattggcgc aaaaggtacc gctatttttt catttcatgc cattaaaaat
     6841 attacctgtg cggaaggagg cctgattgtt actgataatg aaaatattgc ccgccagcta
     6901 cggatgctga aatttcacgg cctgggtgtc gatgcctatg acagacaaac ctggggccgt
     6961 gcaccgcagg ctgaagtctt aacgccgggc tataagtaca atctgaccga tattaacgcc
     7021 gcgattgccc tgacacagtt agccaaatta gagcacctca acacccgtcg gcgcgaaatt
     7081 gcccagcaat atcagcaagc actggcagct ctcccctttc agccattaag ccttcccgcc
     7141 tggccgcacg ttcacgcctg gcatctgttt attattcgtg tcgatgaaca acgttgtggt
     7201 atcagtcgcg atgcgttgat ggaagcgtta aaagaaagag gtattggtac cgggttacat
     7261 ttccgcgccg ctcacacaca aaaatattat cgcgagcgtt ttccctcgct gtcgttaccg
     7321 aataccgaat ggaatagcga acgcatctgt tctttgccgc tgttcccgga tatgactacc
     7381 gccgatgccg accgtgtcat cacagccctt cagcaactcg caggacaata agccatgttt
     7441 gaaatccacc ctgttaagaa agtctcggtg gttattcccg tttataacga gcaggaaagc
     7501 ttaccggaat taatcaggcg caccaccaca gcctgtgaat cgttggggaa agagtatgag
     7561 atcctgctga ttgatgacgg cagtagcgat aattccgcgc atatactggt cgaagcctca
     7621 caagcggaga acagccatat tgtatctatt ttgcttaacc gcaattacgg gcaacattca
     7681 gcgattatgg cgggattcag tcacgttact ggcgacttaa ttattaccct tgatgccgat
     7741 ctccagaatc cgccagaaga aatcccccgc ctggtggcaa aagccgatga aggttacgac
     7801 gtggtaggga ctgtacgcca gaaccgccag gacagctggt ttcgtaaaac agcttcgaag
     7861 atgattaacc ggcttattca gcgcaccacc ggcaaagcga tgggtgacta cggttgtatg
     7921 ctgcgcgcct atcgccgtca tattgtcgat gcgatgttgc actgccatga acgcagcacc
     7981 tttatcccga ttctggcgaa tatcttcgcc cgccgtgcca ttgaaattcc agtgcatcat
     8041 gccgagcgtg agtatggtga atccaaatac agctttatgc gcctgattaa tttgatgtac
     8101 gacctggtga cctgccttac cacaacgccg ctacgtatgc tgagcctgct cggcagcatt
     8161 attgcgattg gaggttttag cattgcggtg ctgctggtga ttttacgcct gaccttcgga
     8221 ccacaatggg cggcagaagg cgtctttatg ctatttgccg tgctgtttac ttttattggc
     8281 gctcagttta tcggcatggg attactcggt gaatatatcg gcaggatcta caccgatgtc
     8341 cgcgcccgcc cccgctattt tgttcagcaa gttatccgtc catccagcaa ggaaaatgaa
     8401 taatgaaaac cgtcgttttt gcctaccacg atatgggatg cctcggtatt gaagccctgc
     8461 tggctgccgg ttacgaaatt agcgccattt ttacccatac cgataatccc ggtgaaaaag
     8521 ccttttatgg ttcggtggct cgtctggcgg cggaaagagg cattccggtt tatgcgccgg
     8581 ataacgttaa tcatccgctg tgggtggaac gcattgccca actctcacca gaggtgattt
     8641 tctcttttta ttatcgccat cttatttgcg acgagatttt gcagctcgct cccgcaggtg
     8701 catttaatct gcacggttcg ctgttaccaa aatatcgtgg tcgcgcgccg ctgaactggg
     8761 tgctggtgaa cggtgaaaca gaaactggcg ttacattgca ccgaatggta aaacgtgccg
     8821 atgccggggc cattgtggcg caactgcgcg ttgccattgc gccagacgat atcgctatta
     8881 cgctgcatca taaattgtgc catgccgcgc gccagctact ggaacagaca ttacccgcca
     8941 ttaaacacgg taatattctg gaaatcgccc agcgcgaaaa cgaagccacc tgttttggtc
     9001 gcagaacgcc ggatgacagt ttcctcgaat ggcataagcc ggcatccgta ctgcacaaca
     9061 tggtacgtgc cgttgccgat ccgtggccgg gtgccttcag ctatgttggc aatcagaaat
     9121 tcaccgtctg gtcgtcgcgt gttcatcctc atgccagcaa agcacagccg gggagcgtga
     9181 tttctgttgc gccactgctg attgcctgtg gcgatggcgc gctggaaatc gtcaccggac
     9241 aagcgggcga cggcattact atgcagggct cgcaattagc gcagacgctg ggcctggtgc
     9301 aaggttcacg cttgaatagc cagcctgcct gcaccgcccg acgccgtacc cgggtactca
     9361 tcctcggggt gaatggcttt attggcaacc atctgacaga acgcctgctg cgcgaagatc
     9421 attatgaagt ttacggtctg gatattggca gcgatgcgat aagccgtttt ctgaatcatc
     9481 cgcattttca ctttgtcgaa ggcgatatca gtattcattc cgaatggatt gagtatcacg
     9541 tcaaaaaatg tgatgtcgtc ttgccgctgg tggcgatagc cacgccgatt gaatataccc
     9601 gcaacccgct gcgcgtattt gaactcgatt ttgaagagaa tctgcgcatt atccgctact
     9661 gcgtgaagta ccgtaagcga atcatcttcc cgtcaacttc agaagtttat gggatgtgta
     9721 gcgataaata cttcgatgag gaccattcta atttaatcgt cggcccggtg aataaaccac
     9781 gctggattta ttcggtatca aaacaattac ttgatcgggt gatctgggcc tatggcgaaa
     9841 aagagggttt acagttcacc ctcttccgcc cgtttaactg gatgggacca cgactggata
     9901 accttaatgc ggcacgaatc ggcagctccc gcgcgattac gcaactcatt ctcaatctgg
     9961 tagaaggttc accgattaag ctgattgatg gcggaaaaca aaaacgctgc tttactgata
    10021 ttcgcgatgg tatcgaggcg ttataccgca ttatcgaaaa tgcgggaaat cgctgcgacg
    10081 gcgaaattat caacattggc aatcctgaga acgaagcgag cattgaggaa ctgggcgaga
    10141 tgctgctggc gagcttcgaa aaacatccgc tgcgccatca tttcccaccg tttgcgggct
    10201 ttcgcgttgt cgaaagtagc agctactacg gcaaaggata tcaggacgta gagcatcgta
    10261 aaccgagcat ccgcaatgcc caccactgcc tggactggga gccgaaaatt gatatgcagg
    10321 aaaccatcga cgaaacgctg gatttcttcc tgcgcaccgt tgatcttacg gataaaccat
    10381 catgaccaaa gtaggcttac gcattgatgt cgataccttt cgtggcaccc gtgaaggcgt
    10441 gccgcgtctg ctggaaatct tgagtaagca taatattcag gccagcattt ttttcagcgt
    10501 cggcccggac aatatgggcc gccatctctg gcgactggtg aagccacagt ttttgtggaa
    10561 gatgctgcgc tcaaacgcgg catcgcttta tggctgggat attttactgg caggtacggc
    10621 ctggccaggt aaagagattg gtcatgccaa tgccgatatc attcgtgaag cggctaaata
    10681 tcacgaagtc ggcctgcacg cctgggatca ccatgcctgg caagcccgta gcggtaactg
    10741 ggatcggcaa acaatgatcg acgatattgc acgcggcctt cgcactctgg aagagattat
    10801 cggtcaaccg gtaacctgtt ctgccgctgc gggctggcgt gccgaccagc aggtgatcga
    10861 agcaaaagaa gcgttccatt tgcgctacaa cagcgattgt cgtggggcca tgccgttccg
    10921 tccactgctc gaatcaggaa accctggcac tgcgcaaatt ccggtgacct tacccacctg
    10981 ggatgaagtg attggtcggg atgtgaaagc agaagatttt aacggttggt tactcaaccg
    11041 catcctgcga gataaaggca cgccggttta taccattcat gcagaagttg aaggctgcgc
    11101 ttatcagcat aattttgtgg atctcctcaa acgcgcagct caggaaggcg tgacattttg
    11161 ccctttaagc gaactgttat cagagacgtt gccgctcgga caagttgttc gcggaaatat
    11221 tgccggacgt gaaggctggc tgggttgcca acaaattgcg ggtagtcgct gatgaaatcg
    11281 gtacgttacc ttatcggcct cttcgcgttt attgcctgct attacctgtt accgatcagc
    11341 acgcgtctgc tatggcaacc cgatgaaacg cgttatgcgg aaatcagtcg agaaatgctg
    11401 gcatccggcg actggattgt gccccatctg ttagggctac gttattttga aaaacccatt
    11461 gccggatact ggattaacag cattgggcaa tggctatttg gcgcgaataa ctttggtgtg
    11521 cgggcaggcg ttatctttgc gaccctgtta actgccgcgc tggtgacctg gtttactctg
    11581 cgcttatggc gcgataaacg tctggctcta ctcgccacag taatttatct ctcattgttt
    11641 attgtctatg ccatcggcac ttatgccgtg ctcgatccgt ttattgcctt ctggctggtg
    11701 gcgggaatgt gcagcttctg gctggcaatg caggcacaga cgtggaaagg caaaagcgca
    11761 ggatttttac tgctgggaat cacctgcggc atgggggtga tgaccaaagg ttttctcgcc
    11821 cttgccgtgc cggtattaag cgtgctgcca tgggtagcga cgcaaaaacg ctggaaagat
    11881 ctctttattt acggctggct ggcggtcatc agttgcgtac tgacggttct cccctgggga
    11941 ctgacgatag cgcagcggga gcctgacttc tggcactatt ttttctgggt tgagcatatt
    12001 caacgctttg cactggatga tgcccaacat agagctccgt tctggtacta cgtgccggtc
    12061 atcattgccg gtagtctgcc gtggctggga ttactccccg gtgcactata cacaggctgg
    12121 aaaaaccgca agcattccgc aaccgtctat ttgttgagct ggacgataat gccgctgctg
    12181 tttttctccg ttgctaaggg taaattgccc acctatattc tttcctgctt tgcacctttg
    12241 gcaatgctga tggcgcatta cgctttgctg gcagcaaaaa ataatcctct ggcgctgcgg
    12301 attaatggct ggattaacat cgcttttggc gtcactggca ttattgccac atttgtggtc
    12361 tccccctggg gaccaatgaa cacgccggtg tggcaaacct tcgagagcta taaagtcttt
    12421 tgtgcctggt cgattttttc gctatgggca tttttcggct ggtacacctt aacaaacgtc
    12481 gaaaagacct ggtcttttgc cgcgctttgc ccgctggggc tggcgttgct ggtaggattt
    12541 tcaattcctg acagagtcat ggaaggaaaa catccgcaat tttttgtcga gatgacacaa
    12601 gaatcacttc agccaagccg ctatattctt actgatagcg tcggtgttgc cgcaggtctg
    12661 gcatggagtc tgcaacgcga tgacatcatc atgtatcgcc agacaggtga gttgaaatac
    12721 ggccttaatt atccggatgc gaaagggaga tttgtcagcg gtgatgagtt cgcaaactgg
    12781 cttaatcaac atcgtcagga ggggattatt acactcgtgc tttcggttga ccgcgatgaa
    12841 gatatcaaca gtctcgccat tccgcccgca gatgccatcg atcgtcagga gcgtctggtg
    12901 ctgattcagt atcgtcccaa atgatctggc taacattagt ctttgccagc ttgcttagcg
    12961 ttgccgggca gttgtgtcag aaacaggcaa cctgctttgt ggcgataagc aaacggcgca
    13021 aacatatcgt gctgtggctg ggactggcgc tggcttgtct tggtcttgcc atggtgctct
    13081 ggctgctggt cttgcagaac gtaccggtag gtattgccta cccgatgtta agtctgaatt
    13141 ttgtctgggt gacgctggct gcagtaaaac tgtggcacga accggtatcg ccgcgtcact
    13201 ggtgtggggt ggcgttcatt attggtggca ttgtgatcct cgggagtacg gtgtaatggg
    13261 cctgatatgg ggattattca gcgtcattat tgcctcagtt gcgcaattaa gcctgggttt
    13321 tgcggcgagt catctgccgc cgatgacgca cttatgggat tttatcgcgg cgctgttagc
    13381 ttttgggctg gatgcccgga tactgttact cgggctgctg ggttatctgc tttccgtttt
    13441 ttgttggtat aaaacactac ataaacttgc gctaagcaaa gcctatgcct tgctcagtat
    13501 gagttatgtg ctggtatgga ttgcttcaat ggtacttccc ggctgggaag ggactttttc
    13561 gctgaaagca ctactgggag tagcctgtat tatgagcggg ttgatgctga ttttcctgcc
    13621 cacgacaaaa caacgttact gagttttccc tgccacttta cactgccgtt cccactcatc
    13681 ggggctataa cagcttgcag agaggacttt caccgtatgc agcttttcac gattaataca
    13741 ataaagcgca ttctgcaaag gcgagagtaa atcccccact ttcacggcga agtcactctt
    13801 cacttcggcg atcattttta tcgcgccgcc agcatcgcag agcataagta catgtctgtt
    13861 atcttgttta ttgcagcacg attttttgac cagccattcc attgtattat cctgtttgct
    13921 aagagttttc atatcaattt acagattaac agataaactc tttatcaacg gcaatggaat
    13981 ggcagaactg agaagtgaaa cggagggtta attattgctg acgttgcacc cactctttta
    14041 gcgcctgacg tgaaatttta ataccgccgt ttttcagctc cggcggcaaa gtcagccaac
    14101 gtaccggttg ctgaaaacgg gccagcttat ctttcaccca ttcactaaga tcaacgctct
    14161 cgtggtcata ctccatcacc gccaccggtc gatgaccaaa ctccttgtcg gcaacgggga
    14221 cgataaacac ctgcaaaacc gcaggatgtg cagcaattac gcgctcgact tcttccgtct
    14281 gaataccctc tccgccactg aagaatagat tgtctaaacg tccgacaatg gtcagcttgc
    14341 cattatgcat ctcaccgcga tcgcgcgtag cgtaccagcc ttcgtcatta accagtgaaa
    14401 ccagttgccc gttacgccag taaccttctg ccatactggc agcccgcagc cacacttcat
    14461 tattaacgat tttcacttcc cgacccggca gcggcgaacc aacgtctgcc aggccgtcgg
    14521 cttctttcgc acacaccgtg gaggcaaact cggtcagacc atagccgcaa aagcaacgaa
    14581 tcccctgctc gcgcgcctgt tccgtcaact cgaccgggat agccgcgccg ccaagtaaca
    14641 ccgctttcag ggaaacggaa ctgcggttaa ccagcaaacg ccagagttgt gttggcacca
    14701 gtgaagcgtg agtacagcct gccagcattt gctccaatgg ctgtttatca cgcaccgtca
    14761 tccgcgcacc agcgtataac cagcgccaca taattccctg accggagacg tgaaacagcg
    14821 gtaaagagag caaccagtca tcgtgatcgc caaacggaat cagcgataac accccttcgg
    14881 cactggcaag atgggcctga taagtatgga cagcggcttt cggcaacccg gtagaacccg
    14941 aggtcaacgt cattgagcac agacgcgtcg gctgccacgt agcgacatgt gcgccttcaa
    15001 cccgctgaat gtgcagcgac gttaatgccg gaaacgtgtt ttccccatcc ggcaccagcg
    15061 caaattgcag cgtcagattg ggcagcaatt cttcaagcaa cggttgcggc agctgagggt
    15121 tcacgggcaa cacccgcgcc ccgcattgca gtaacgccag ccaagcgagc agcgtttgcg
    15181 gcgtattcca cgcccgcaac atcacgccgc tgtcctcaac cacaccctgc accgcaaatc
    15241 cggaggctaa ttcatcgacg cgagcacaaa gctcgcgcca gttgagttgc tcgtcattaa
    15301 gacgtaaggc gatggcttct ccccgcactt gccgccagtg acgccacggc cagtcagaga
    15361 agatcataac aaccgctcca gtgcatcaac ttccacgaca ggcagcgtgc tacccggcca
    15421 gcgacgtacc tgctgcgcct gcattagatc cagcgtgtcc agccctggaa tggtgtccgg
    15481 cgttaaccag gcagcaatcc gcgccagttg cgttaagcct aagctcgatt caatggaaga
    15541 actgatcact gccgtcagcc ccagcgcgtg cgccgcctgt acctgctcgc gaactttctc
    15601 cagactgccc gtgagcgtgg gtttgataac taccgcgcgc acgccctctt cagccacaaa
    15661 ggcaaaatcc ggctcgcgca agctttcatc ccaggcaatg gcaatgccgg tttcacgggc
    15721 aaacgctcgc gaatcatcgc gggttttgca cggctcttcg agaaacgcga tgcggtggcg
    15781 ataatccggg ttaacgtatt tggcaaactg ctgacctttc agcggtgtcc aggcgcgatt
    15841 tgcgtcaaga cgcaaatgca gatccggaat tgcctccagc aacagattca ccaccatgcc
    15901 gtcgcgcacc gcttcgtaca atcccacttt gaccttcgcc actttctcgc ctggcatatc
    15961 tgcaagtttg aggatcagat cgtccggatc gccattacac agtggtgccg tacggtaatt
    16021 ggccgcctgc gggagcgtat ctgccagctc cgccaatgca caacttacgc caaaggcaac
    16081 ggaaggcatc tgcggtaatt cgcaatcgcc tgccagccag ttatttaccc aggcaagcag
    16141 cacactttgc gcctcttccc aggtttcctg actgaagccc ggcagtgggg agatctcccc
    16201 ccacccttcg cgctcgccct cacgcaggca aacgtacagc ccgtcgcggg tttttaaccg
    16261 cctgtcgcgc agaaccaccc ccgcgtccat ggggatctgc cagcggtata cctgcgcgct
    16321 acgcattacg gattccgttt gaatttgctg aagtcaggct gacgtttctg gttgaaggcg
    16381 ttgcgacctt cctgaccttc ttccgtcatg tagaacagca tggtggcgtt gcccgccagc
    16441 tcctgcagac ctgcctgacc gtcacagtcg gcgttcagtg cagctttcag gcagcgcagc
    16501 gccatcggac tgttttgcag catttcacgg caccagcgaa cggtttcttt ttccagatcc
    16561 gccagcggta ccacggtgtt gacaaggccc atatccagcg cctgttttgc gtcgtactga
    16621 cggcacagga accagatttc acgcgctttt ttctgcccga cgatacgagc catgtaggaa
    16681 gcgccccagc cgccgtcgaa ggaaccgact ttcgggccag tctgaccgaa gatggcatta
    16741 tctgccgcga tagtcaggtc gcacatcatg tgcagaacgt gaccaccgcc gatggaatag
    16801 ccagccacca tcgcgacaac cggtttcgga caggtacgga tctgacgctg gaagtccagc
    16861 acgttcaggt gatgtacgcc ggaatcatct ttatagccgc cgtaatcacc acgcactttc
    16921 tggtcaccac cggagcagaa cgctttatcg cctgcgccag tcagaatgat cacgccgacg
    16981 ttatcgtcat aacgcgcatc tgccagtgcc tggatcatct cttttaccgt cagaggacgg
    17041 aaggcattgc gcacctgcgg acgattaatg gtgatttttg cgataccgtc ggtggatttt
    17101 tcataacgaa tgtcctcaaa gccttcggag cagtcgtgcc attcaaccgg tgcgtaaagc
    17161 attgcttcat caggataaat catattgtgt cctttagtca gaaacgcaag atctgcgcca
    17221 gacttgcgat tacgccagcg ggattttccc gatgcgcgtt atgtccggcg cgaggaatga
    17281 catggcagtc ggcagccagt tccgccgcca gggcgcggaa tttgctgtca cgttcaccac
    17341 ataaataata aaacgcaaat gtgcgggcgc taaggttagc acgtaaatca ggctggacgg
    17401 cgagagaagt cgcctccagc atggcggcaa gggttgcgcc attattgttg ctgcgcagca
    17461 ccaccagctc ccggcgttga tcgtcattga gcgaggcaaa aacaggctgt tgataccagt
    17521 cggcaaatac cgcggttaat ggttcggaac gaaaacgctg cgcccattgg cgatcggaac
    17581 gctgacgttc cgcgcgttgt tcagcatttt gcagccccgg atgaccgcct tcgacaacaa
    17641 ccccacaaag ccccgccagc ccctggcaag cggccatcat cgccacccgt ccgccaagcg
    17701 agtaccccac cagccagaag ctaaggatgt tgtaactaac cagggtttta cgcagtaagt
    17761 cggtgacatc atcaaatcca tcgacgctaa tcgccgccga accaccgtga cctgggagat
    17821 caacatacaa ccgtgagtag tcggcaaacg cctcgccgac ttcctgccat tcgaggcaat
    17881 cgccggaaaa accgtgcaaa aacaccagcc agggtaaacc tggtttgccg tgttttgcct
    17941 gcgcgtgcag gatcataaat ggcttacctg cgccagcagc tgctggagcg tttgcgcgcc
    18001 gtcggtgtcg ttaaccacca tttcaattac cgtggtggtc ggagtacgcc aggcgtcggc
    18061 aagtaccgtt tcaagttcct gccagttttg cggacgatga tatttcagct caaacatcgc
    18121 ggcagcgtgc tcaaaatgga cgttttgcgg catcagatag aaacgctcgc gctcgctttt
    18181 cggcgttggc agcagcgaga aaatttgccc gccgttgttg ttcaccacaa ttaataccag
    18241 cggcgcggaa acctggcgca ataacgccag cgcattcagg tcgtaaagtg cggagagatc
    18301 gcccacaatc gccagcgtcg gtttgccgct tgcccgctga acgccggcgg cagtcgagag
    18361 cagcccgtcg ataccgctgg ccccacggtt gctgtacacc gggtaacctg ccggaagttg
    18421 cgaaagcgca tcaatcagac gtaccaccag gctgttaccg acaaacaatt gcccctgttc
    18481 aggcagatag tcgctgatgc gatgcgccag ttgcgcttcg ccaaacgcat cgcgacaggc
    18541 aataaccgcc tgcattgcct gttccgccag gcgcgggatt tcaacgcacc agggctggcg
    18601 tttttctgcc ggatgcagtt ccagccagtc ggcaatattg gcaattaagc ggcgtccacg
    18661 atggtgtgcc ggatcaagcc gcccttcgat gtcatccaca atccagtact cttcgggttc
    18721 acagcttgcc tgccattgca ggagccgttt gcccgtcagg ctgcttccca gttgcaccac
    18781 aatttgcgcc tgttgcagtt cgctggtcgc tttggcattg cctaaccaga gatcggcaca
    18841 cggcagcggc tgtccggttt gcgacagcac gtcgccaatc agcggccagc caagagtttg
    18901 cgcccacagg gcaacttttt tgccctcttc cgcactcatg cgcccggcaa ccaccacgcc
    18961 gcgcttttgc cgccagaaga accagtcgcg ctgtttttcg ctctcaagac gaggcgcttc
    19021 acgcagccac ggcttatcat cctgccacca gtcacccaga cgctgttgcc agctaagccc
    19081 agtgtcgtcc atctcgccat acagcggttc agcaaacggg cagttgatat ggaccccacc
    19141 agcatgaagc gtaccgagag catggtcgat ggtagaaacc agccaacgtg cggggatatc
    19201 ctgggtcggg cgcggcagtg aaatactgtg cgtggggtga gaggcgaaca ttcccggctg
    19261 acgaatcgcc tgattcgcgc cgcagtcaat tagctccggc gggcgatcgg cggttaacag
    19321 gatcagtttt tctccggtta acccagcttc aatcagtgcc ggatagagat ttgctaccgc
    19381 cgtgccggag gtcacaatca ccgccaccgg ctgcttgctg actttcgcca gccccagcgc
    19441 cagatgcccc agtccacgct catcgaaatg ggtgtggtga atgaatgcgg aattctccgc
    19501 cgccgctaac gttaacggtg tagaacgcga gccaggggcg atgcagatgt gtcttacgcc
    19561 gtgacgcgtt aatgcttcca gaatgaccgc cgcccagcgt cggttaaatg cgcttactga
    19621 catgagtttg tccggtatca atattgcggc taagtataag gagctacaaa aatagattat
    19681 tgatatgaat cggtaatgat gcgactcatt actattccat ttgtaataaa gtacgcagcc
    19741 ctgccgcttt gttgtcgatt tcctgccact cttgctcggg gtcggaacca cggacaatgc
    19801 ccgcgccagc atatagccgc acgacattgc cgctgatttt tgctgagcgc agggaaacgc
    19861 agaattcgct ttgttgtaat gagagatagc ccgcagaacc ggcgtaccat tctcgggtga
    19921 acggttcgtg atgggcgata aactgtcgcg ccagatcgcg cggtaagcca gcaactgctg
    19981 ccgttggctg caactgatgt aaacagatca catcatccgc tttgttgagt gaagtccaga
    20041 tacagcggcg aagatgctgc actttacgca gacgcagtac ctgcggcggt aaaacatcca
    20101 gcgtctgggt atcggcctgt aatcgttggc agatatcttc caccaccagc atgttctcgc
    20161 gctggttttt atcatccgcc atcagccact ctcctaactg ctgcgcctgc ttatcatcag
    20221 gatgatttgc tactgttccc gccagcgctt cagtacgcag cgctttgtca cgccgccgcc
    20281 ataaccgttc cggtgaagag ccaagaaaag cattttcgcc atcaaaggcc atgtaaaaat
    20341 ggtagcaatt gagattcagt cgacgactgg cagccatcat cgccgccgca ttgaccggac
    20401 ttgcgaaatg caggtcagtt gcccgagcaa gcaccacttt gtcgagctca ccttcggcga
    20461 tggttttcgt tgccagttcg attaattgcg tccagcccgt tttgtccggc cagtgttgtt
    20521 ctcgcgtagt ggttaaatgt aacccaggca agggcttgat actcaccagt gtggcgataa
    20581 attctcttgc cttaatcgca tcgtgctgaa gggagctttc gctgaatagc gtcagccgca
    20641 gcgtggcttt accgccacag cgtcgccatt ccaggcgtgg taaaagtaaa ttgccctgcg
    20701 acgggtcaaa cgcattcagc ccccaaatgc gtaagtcggc gtgttccggg tgctggcgaa
    20761 gaaaacgttg tgcctggtcc aacgacgtaa aacgggtaat cgcgcccagg acggcagctt
    20821 cttcatcacc attacgttgt tgccagtaga attgcgggta cgtttgctga ctggccagcc
    20881 agctcaaggc atcaaaagcg tctttgagag ggaaaggaat atcgataacc cgaatgccgg
    20941 gtgtcgccgg aatctcttgc gacaaatggc gcagtagatt ttccagcgcc gtagtaagtg
    21001 attgcacgcg gacctctccc tgttaaaagc ctcacattat acggggtact acaaaaaaaa
    21061 tgcagtaccc cggtgtaggg aggttttaac ggcgtgccag caacagtcct agtaccagcc
    21121 caacggccgc acccacacca attccctgcc agggtttttc gtggacgtag tcatcagcac
    21181 gataaacagc ctgcttcgcc cgatagtaat aactgtctga agcctggcta acccgttttt
    21241 tcacatcatc cagcgctttt tctgcacgcg ctttcagctc aacatatttc tgatcggcgg
    21301 gatcgccaga ggagcggagc acctcttcca gtgtttcact aagcagcgtc aggtcgtcat
    21361 cgatacgtgt atcaccaaac tgattagaca ttctcgttct ccatttgcgt aaaacctgtc
    21421 actaactata gacaagggtt attgattacg cctgaattac ctcgcgtgcc atcccaatgt
    21481 gtgggatacc atcttcttca tagatctccg tcacagggat aaaaccgaaa ctctggtaga
    21541 agttttgcag atgcgcctgc gccccgaggt agacaggctt atcaggccag tgatgcgtgc
    21601 aagtgtcgag ggttttactc atcagttgct gccccacttt ttcgccacgc aacgcttcac
    21661 tgacaatcac ccgaccaata acgaccggct caagatcatc atcacttttc agaatcctcg
    21721 catatgccac cagttcatca tttttccaac cgagaatatg gcgattatcc cccgtcaaat
    21781 catcaccatc gatatcctga taagggcaat tttgctcgac aacaaacacc gcgcaacgca
    21841 gttgtaataa ggcataaagc tgggaaaccg ataattcaga gtgatgcaga tcttgccatt
    21901 caatcataat gctctccttt tagatgtatg ccttatacta gccggaatat tttttgaaac
    21961 gtgatgaact catggaatta atttttttag gtacttcggc cggtgtgcca acccgcacgc
    22021 gcaatgtcac ggcaatattg ctaaacctgc aacatccgac ccagagcgga ctttggttgt
    22081 ttgactgcgg tgaaggcacc cagcatcagc tactgcatac cgcctttaac cctggaaaac
    22141 tggacaagat ttttatcagt caccttcatg gcgatcatct ttttggttta cccggcttgc
    22201 tgtgcagtcg ttctatgtca ggcattatcc aacccttaac gatttatggt ccgcaaggta
    22261 tccgtgaatt tgtggagacc gcgctgcgga ttagcggctc atggaccgat tatccgctgg
    22321 aaattgtcga aattggcgct ggcgaaattc tcgatgatgg cctgcgcaaa gtgaccgctt
    22381 atccaatgga acacccactg gaatgctatg gctatcgtat tgaagaacat gataaaccgg
    22441 gtgcattaaa tgcccaggca ttaaaagctg ctggcgtacc gcctagccca ctgtttcagg
    22501 aattaaaagc gggcaaaaca atcatgctgg aagatggaag gcagattaac ggcgcagatt
    22561 acttagctgc tccagtgcca ggtaaagcgc tcgctatttt cggcgatacc ggcccctgcg
    22621 atgccgcact tgacctggct aaaggtgtcg atgtcatggt gcacgaagcg acgctggata
    22681 taaccatgga agccaaagcc aatagtcgcg gtcatagctc tacacgccag gctgcgacac
    22741 tggcccgtga ggctggagtc ggtaagctaa tcattaccca cgtcagctcg cgctatgatg
    22801 acaaaggttg tcagcacctg ttacgtgaat gcaggtcaat tttcccggcg actgaactag
    22861 cgaatgattt caccgtgttt aacgtttaac tctgcttatt attaaaaaca gggcgaaact
    22921 tgccctgtta tcgcaacccg cgctacatat atccactcac acgggcatat tgagcgattt
    22981 ttgatattta ttcatctcct tacgcactat attcagagca ataagttgcc tctttttatc
    23041 ttcattcagc acaacctttg caactaaaag gagcatatga tggttacagt tgtcagcaat
    23101 tattgtcaat tatctcaaac ccaactcagt cagacatttg cagaaaaatt tactgtgacc
    23161 gaggaattac tgcagtcttt aaaaaaaaca gcgttatccg gagatgaaga aagcattgag
    23221 ttactgcata atattgcgtt aggttatgat gaatttggga aaaaagctga agatattctt
    23281 taccatattg ttagaaaccc aacaaatgat accctatcga ttatcaagct tataaaaaat
    23341 gcctgtttaa aattatataa tttggcacat accgcaacca aacaccccct caaatcacat
    23401 gattcagata atctcctgtt caaaaaacta ttctcccctt cgaaattaat ggcaattatc
    23461 ggtgaggaca ttcctctcat atcagaaaaa cagtcacttt caaaggtact t
//
LOCUS       DBFIFM010000062        23397 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.062, whole genome shotgun sequence.
ACCESSION   DBFIFM010000062 DBFIFM010000000
VERSION     DBFIFM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.062"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..444
                     /locus_tag="N7C74_002070"
     CDS             <1..444
                     /locus_tag="N7C74_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU2431942.1"
                     /translation="LCHVHHKNGIKYDNHHENLLVLCKDCHRKQPLHEGIFVTQAEMA
                     IIQRLRSQQGLLKAESWNEIYDLTDPSVHGDINMMQHKGFQPPVPGLDLPNSEHEIIA
                     TVEAAWPGLKIAVNLTPAEVEGWRIYTVGELVKEIQTGAFTPAKL"
     gene            669..911
                     /locus_tag="N7C74_002071"
                     /pseudo
     CDS             669..911
                     /locus_tag="N7C74_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
     gene            985..1836
                     /locus_tag="N7C74_002072"
     CDS             985..1836
                     /locus_tag="N7C74_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HGU2431943.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGTEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGIWQLHNAERCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QTRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALVAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EGFVGER"
     gene            complement(2034..2221)
                     /locus_tag="N7C74_002073"
                     /pseudo
     CDS             complement(2034..2221)
                     /locus_tag="N7C74_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611456.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2341..3123)
                     /locus_tag="N7C74_002074"
                     /pseudo
     CDS             complement(2341..3123)
                     /locus_tag="N7C74_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008815333.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(3839..4297)
                     /locus_tag="N7C74_002075"
     CDS             complement(3839..4297)
                     /locus_tag="N7C74_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2431944.1"
                     /translation="MTLRKLFGQRVKELRIATGMSQEAFADRCGFARSYMSRIERGGS
                     NASLDAIEVLANALSVEPWRLLVSDSSEDSDPELLVPYAADGSCFHPGLASTRDGSFG
                     VGDKTAQKRFGTFSEALEYLRSMETAKWRRPNPSGNWGIVSAVRWDKLRK"
     gene            4420..5193
                     /locus_tag="N7C74_002076"
     CDS             4420..5193
                     /locus_tag="N7C74_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2431945.1"
                     /translation="MSKTRLSKPGFSAKSVLTIMLIAASSASYAAESIDQLATRIGMD
                     HFREAIKKGERSAFKTLPAKGYKNGLTGLLIKTNTYVNPEYSFADCDTDTVIISVQEH
                     KTLPGCRISLKLATKDADGSYHDNGIRVEYGIAIVSKKFIANNSAWAQHAISGDKVLE
                     GALHPDKTNNNKDQMNNNKDQMCMGMAQFTANVTEGRNSGMTKREYYQRLDNMNGKAD
                     VTQQMITLYKTFVDYVYEHPKQDAKISGTYIYTNCMNNF"
     gene            5345..6661
                     /locus_tag="N7C74_002077"
     CDS             5345..6661
                     /locus_tag="N7C74_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2431946.1"
                     /translation="MNDGVMLDSDVIAGLEWLASTQENKQHFYQRLAKAQQFYIATTQ
                     KAANFGKQFDPEWYGSDVVAGYFAQAKSLIDNRRSYEVTNASNIIPWVKQLGVCAKSL
                     DRITGARDRAIRMLKNTTVLPDTALLELVLAGNYASEGYEVEFIPEQKGIAKTPEFRC
                     RLGDGDYFFVECKRLQKGPYVKQEILQHHIRSHLAELHIKSKKMNVWTDVTYLCEVKD
                     APENYIISHLKNFKGVFYEWNDDYGKGTIRPANLNLIREDITENGSLLVNTKLARLIK
                     GSPLEDENYQVVAMGRPDERDSRFITKVKLASLITWRCINEKSFDARSRHVTKTLAEI
                     DNQLLDYGLGVGHIALDVDIQKDVADKRREKNYAAIVNFEQKSKIVRLNVHYLVPRID
                     ENSAWMVDETADDFFTHDLVKYVIPLTKIFPEAEVFENDQPGWHQN"
     gene            complement(6860..7429)
                     /locus_tag="N7C74_002078"
     CDS             complement(6860..7429)
                     /locus_tag="N7C74_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU2431947.1"
                     /translation="MAIFGYGRVSTSQQDTENQRMELEQAGWTFDFWFSDVVSGKVPA
                     VQRKAFFEMLSKIRDGETLVVAKLDRLGRDAIDVLQTVRTLADRNIKVIVHQLGTTDL
                     TSAAGKLLLSMLAAVAEMERDLLIERTNAGLLRAKAEGRKLGRPAKIAPEARGAILDK
                     RAAGVSVSALAREYGVSRATLAALLNKGY"
     gene            7622..8089
                     /locus_tag="N7C74_002079"
     CDS             7622..8089
                     /locus_tag="N7C74_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HGU2431948.1"
                     /translation="MIKDAIFSPCGKYRYSLSRVWDESKPYTLFIGLNPSYADAEKDD
                     RTLSRCISFAKSWGYGGVYMANLFAFVHTQRHEMMKASDPIGKDNDSHLIRLVSGAGL
                     VVAAWGNEGRHLKRSTTVRQLLPESTMCFVLNATGEPKHPLYMKNDSVLIPLG"
     gene            9093..9425
                     /locus_tag="N7C74_002080"
     CDS             9093..9425
                     /locus_tag="N7C74_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HGU2431949.1"
                     /translation="MRTVEILQYTLRKGSGAAFHAIMQEISVPLHQSHGIDVVSFGNS
                     LDDLDCYYLIRAFDSAKSMTAVLDAFYASADWRSGPREDIIGSIENCIKTVISLPSES
                     VKGLRMQS"
     gene            9547..9924
                     /locus_tag="N7C74_002081"
     CDS             9547..9924
                     /locus_tag="N7C74_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HGU2431950.1"
                     /translation="MNLDEEFYELQKLFAQKDLLTEKRRSSGGGFMEILLVKRQNMKI
                     KIYQEKGHQLPHIHIDYGKQAHAASYAIQSGDRIEGDLPKKYDSDVSSWLGRNRDKIL
                     EIWNALQAGAPYEPLIAELTGGV"
     gene            complement(10051..11970)
                     /locus_tag="N7C74_002082"
     CDS             complement(10051..11970)
                     /locus_tag="N7C74_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6538 domain-containing protein"
                     /protein_id="HGU2431951.1"
                     /translation="MSQRYKLYRRTSGIYVVRISVPQRFRRYAGQCEIHTSTGTHDLH
                     EAKQKSALLLAVWYQTLQEYEQLDYRTLSDCAPLLAGEGMISLSNFAQSIELPISQLI
                     REVVNRNLPVFWLATGQFGFYVDEFNAVEREPGAKREKQSDDEKDQPKEVIILNSAFE
                     LGIESFANGYLRPFNPRHTLDCLLSAGVSEGEAAFRTSGDNQSGGWFFDLPGVDITAD
                     SLLISKVHAEGLRLTWLVKTTPPAVSIHPAVPLVAPVIANEYVHRKHYNENLSWLREE
                     YLKHRRKGKVSEAALRDIRYYFDLMIEVMGDIQLEDFDRDFLRAYESKLRTIPANRNL
                     MKGKHGVKTLDELIAKAAECGDKLMTEESVKKYINGLYGAMEWAVDDGKFLKSPCDNF
                     FPPDDKGEREQDHTDIFEPHEIKAIFSQPWFVAGTVERNAQGRFHQYCPFHYWAPLLG
                     LMTGARVNEIAQLMLDDVLADDGVYYLNLESDSENGKKLKNANSRRKIPVHSTLIELG
                     FIEYVDALKAAGYDRLFPELKPHKTKGYGRPVSAWFNESLLAGRLKLERDRSKSFHSF
                     RHSVSTLLKEKGVSSELRGQLLGHVRGKTETEVRYSKDLKPVHMVEVVEKIDFSLPEI
                     ARFNIPDGLDAVELI"
     gene            complement(12061..12145)
                     /locus_tag="N7C74_002083"
     tRNA            complement(12061..12145)
                     /locus_tag="N7C74_002083"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12109..12111),aa:Leu,seq:caa)
     gene            12341..13360
                     /gene="ahr"
                     /locus_tag="N7C74_002084"
     CDS             12341..13360
                     /gene="ahr"
                     /locus_tag="N7C74_002084"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HGU2431952.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLPVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            13490..14992
                     /gene="yjgR"
                     /locus_tag="N7C74_002085"
     CDS             13490..14992
                     /gene="yjgR"
                     /locus_tag="N7C74_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HGU2431953.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIDVNDWQPHANPVVVWDIFG
                     EKGHSVRATVSDLGPLLLVRLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(15153..16235)
                     /gene="lptG"
                     /locus_tag="N7C74_002086"
     CDS             complement(15153..16235)
                     /gene="lptG"
                     /locus_tag="N7C74_002086"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HGU2431954.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(16235..17335)
                     /gene="lptF"
                     /locus_tag="N7C74_002087"
     CDS             complement(16235..17335)
                     /gene="lptF"
                     /locus_tag="N7C74_002087"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HGU2431955.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(17398..17448)
                     /gene="ytgA"
                     /locus_tag="N7C74_002088"
     CDS             complement(17398..17448)
                     /gene="ytgA"
                     /locus_tag="N7C74_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HGU2431956.1"
                     /translation="MSIIILATISTTQENK"
     gene            17602..19113
                     /gene="pepA"
                     /locus_tag="N7C74_002089"
     CDS             17602..19113
                     /gene="pepA"
                     /locus_tag="N7C74_002089"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HGU2431957.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            19467..19910
                     /gene="holC"
                     /locus_tag="N7C74_002090"
     CDS             19467..19910
                     /gene="holC"
                     /locus_tag="N7C74_002090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HGU2431958.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            19910..22765
                     /gene="valS"
                     /locus_tag="N7C74_002091"
     CDS             19910..22765
                     /gene="valS"
                     /locus_tag="N7C74_002091"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HGU2431959.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(22820..>23397)
                     /locus_tag="N7C74_002092"
     CDS             complement(22820..>23397)
                     /locus_tag="N7C74_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HGU2431960.1"
                     /translation="SNTSFGIHRFKVKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQ
                     ILNAYDSSVYANDDIENLQQFMEMQRKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNL
                     SLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPLLKIWMIDWQAKNTYLLGDL
                     DDLPLINKEEQPDKGFLASISRGIMPSLPFL"
BASE COUNT         6301 a         5707 c         5750 g         5639 t
ORIGIN      
        1 ttgtgccatg tccatcataa aaatggcatc aaatatgata atcaccatga aaaccttctt
       61 gttctgtgca aggattgcca tcgaaaacag cccctccacg aaggtatatt cgttacccaa
      121 gcagagatgg ctatcattca acgtttacgt tcccaacaag ggttattaaa agcagaatcc
      181 tggaatgaaa tatatgacct gactgatcca tcagtgcatg gtgatattaa tatgatgcaa
      241 cataaaggct ttcaacctcc tgttcctggg ttagatcttc caaactcaga acatgaaatt
      301 attgcaaccg tagaagctgc atggccaggc cttaaaattg cagttaacct tactcccgcc
      361 gaagtcgaag gatggagaat atataccgtg ggtgagctgg ttaaagaaat acaaacagga
      421 gcctttacgc cagcaaaatt gtaaattcta aaactccgtg aaagttaaga ctttcacgga
      481 agataaataa aatttccctg atttgtggct caaattacaa aagtagttta tggcataact
      541 tgtctgattt ttatggtgta acaggtataa aagcatatgc tatggttcgc ctcatactta
      601 aaacttccct catatgggtg aaggttaaag cttggtagac agaagacagt cacaatgaat
      661 aaagcaataa attgatgtcg cacgcgccga tgggcatggc gggtaatgtg acttttgtac
      721 acaacggcaa ggcttatgtt actggcggtg ttaaccagaa catcttcaat ggctattttg
      781 aagatctcaa cgaggctgga aaagattcag cagctataga caagatcaat gcccattatt
      841 tcgacaaaaa agcagaaaat tatttcttca ataagttttt gttgtctttt gatccctcaa
      901 cacagcaatg gatcttcact tgcaggcttg aacagcagaa aatttgcgag catgattgca
      961 attatttcac tgcctgggat atcaatggct tccagtaaat cgctacagca ggcaattgcc
     1021 aacataaaaa tctggcacaa gggtgaacag cgcgcgccgc ataaaccatt gttactgcta
     1081 tacgtattag cgggatacct gaatggacat ccgcgccttt tcgattatgg cacggaaatc
     1141 tacgaacctc tgcacagttt actggaacgt tttgggccac agcgttcaca gtaccgacct
     1201 gatatgccgt tctggcgatt acaaggcgac ggaatctggc aattacacaa tgccgagcgt
     1261 tgctcaacag cagggagcag ccgacaaccg ccagtgaagg aactgaacga ataccacgtt
     1321 gcaggcggtt tcgatgaaca gcactacgcc ctggtaaccg gcaataaaaa actcatcaat
     1381 actctggcgc aacagatact cgaagcgcac ttcaccgaga gtattcagga agaaatcgct
     1441 gacgagctgg gttttgatct ccagcaaacc cgtaaacagc gcgacccgtt atttcgcaaa
     1501 aatgtattac gcgcttataa ctaccagtgc gcaatatgtg gctttaatat gcgccacgat
     1561 gacaccaccg tcgcgctggt agcggcgcat ataaaatgga aacagcacgg cggcccttgt
     1621 gaaatcccca atggtctggc gctttgcgca atacaccata aagcctttga taaaggatcg
     1681 attggcctgg atgaaaatat gcgggtactg gtctcggatg cggtaaacgg cggagggatc
     1741 gttgagagat tattctggga ttttgacggg aaaactattg cgttgccgca ggtgcgtaaa
     1801 aattaccctt atgaggggtt tgtgggtgag agatagacat tctgtttact aataatttat
     1861 agccattctc tgctcctgca tcagagatat gatgcaggaa tattaaatac cagccaccta
     1921 taaattatga tttattaatt tctgcttcag ttaaatccct ctgaaaatag acccgtttta
     1981 gttccataca tctttaaaaa tcccgaccaa ataatgagat cagcaaacat ctatttgaaa
     2041 cgttggttct catcctcaag atccttgatt attttaatat cagaaaattc cataccgccg
     2101 tatctggact tgcagttgta gtaagtggct tcagataagc cacatcttta acagttcgcc
     2161 cgtattcaag cgatttaatc acggcgataa tctgatgctc agtaaaaggg gctttacgaa
     2221 tagcgatctc cttcattggc agattgatta tgccggagga tctctaaatg tgaatggcac
     2281 gattatgcgg gatatttact gtaccagtcc aaccctctgc ttactgcaat accaatcaag
     2341 agggatggtc cacattgacg tttcaaaatc gatttctgac cagcgagcaa aatgcagctt
     2401 actggaccga ataaagataa gcagagtgag ttctgtcgta cagtgggtta gcggtctgcc
     2461 ggtgtagctg tctattttgt caagcaactc agggatacgc tttaactcca gcgccggacg
     2521 atgctgtcga ttactggaag caaccgcccc cgacatctct tgcgccggat cgtaatcaat
     2581 taacccgctt tgcactgcat aacgcatgat ggccgtggtg cgctgttgaa ggcgggaagc
     2641 cacttcaaga cgcccggatt tctccacggc tttaatagga attaacagat cacgggtgcc
     2701 tagttcagca atgttgcgag taccaattgt cgggaagata ttgtcctcca ggctcttttt
     2761 tacacgatta gcatgatctt ccgaccattt ttggttggtt acgagccact ctctggcaac
     2821 cttctcgaaa gtaataatct ctttcgcctg ctcttctttc acagcacgct tatgctcacg
     2881 aggatcgata ccagcagcta ccagctttct ggcctcttcc cttttcttcc gcgcatccgc
     2941 cagtgaaaca tccgggtaaa cgccgaacgc catcaaatgt tgtttaccgc caaaacggaa
     3001 acgaaatcgc caatactttg aaccgccagg ttttacaagt aaggacatgc cgtcgccgtc
     3061 aacaagtgaa tactctttct cctcaggttt cgcagagcga actttagtat ccgttagggc
     3121 cataatggtc ttccttccat tggtatctcg tgagtataca agcaatatcg aatcggcaaa
     3181 tatactcatt agtatactca caagcctgtt gatgtgggtt ggttcaggtt gacttcagtt
     3241 gaggagaaaa gctggaaaag ccttgcgtgg cgcggatttc aggcataaaa aaagacctca
     3301 gttgaggtct atttacatac ttttggtgcc gaaggccgga ctcgaaccta agtctccata
     3361 acctgctgta atcacgttcc tattagcctt taaaagaaca atcgtaggac ctttttgttc
     3421 taggggctac atcttaagtt tgatccgctg gacggaatca aaccgagtgt tcgtggcggc
     3481 acaaatcatt aacgtgccat acctgccttt tgatgtttat ggaggtctag tgggtcggga
     3541 ggaaagaaca tttgtcagct atgtggatgt taagtaaacg ccactgcgtt cggattttga
     3601 aaccagttaa accccgtcag accttgttca gaataactat ttaactcaat gttatttata
     3661 tgaattaagc cattcagcgt caaagtaaaa cgccaccgta acgcctacgt aacgcacccg
     3721 cattttgctg ttttattcac tcttatagaa acaaagagct gatgaaacgt gaggctatca
     3781 gcatctaatg aacactgttc atccatacag catgaaaatt cataaactta cacttgagct
     3841 actttctcag cttgtcccac cgaaccgcag atacaatacc ccagttgccg ctaggattag
     3901 gcctacgcca ttttgctgtc tccatgctac gaagatactc aagagcttca gaaaacgtgc
     3961 caaaccgctt ctgagccgtt ttatcaccta cgccaaacga gccatcgcga gtgcttgcca
     4021 gcccgggatg aaaacacgag ccatcagctg cataaggaac cagtaattcc gggtcactat
     4081 cttcagatga atcagacacc aatagtcgcc acggctcaac actcagagca ttagccagca
     4141 cctcaatcgc atccaaagac gcattagagc caccgcgttc aatgcggctc atatagctac
     4201 gagcaaaacc acaccgatcg gcaaaggctt cttggctcat tccggtggca atgcgcaact
     4261 cttttactcg ttgtccaaac agttttctca gggtcatttt catgacctga atttgcgcag
     4321 atgtccacta aagagccacg ctccaatagt gacattttga atcaatggtc tatgatgcga
     4381 taactgacac cgctggggtg gattctgagg gataacaaca tgtcaaaaac acggcttagt
     4441 aagcccggtt tttcagccaa gtctgtgctg accattatgt taatcgctgc gtcatctgca
     4501 tcttatgcag cagaaagcat cgatcaactg gcgacgcgca tcggtatgga tcacttccgg
     4561 gaagcgatca aaaagggtga acgcagcgcc ttcaaaacgc tgccggcaaa aggctataaa
     4621 aacggtctta ccgggctgtt aatcaaaact aacacttacg ttaaccccga atacagcttt
     4681 gctgattgcg atacggacac cgttattatc tcggtgcaag aacacaaaac gcttcccggt
     4741 tgccgtatat ctttaaagct agcgacaaaa gacgcagatg gcagttatca cgacaatgga
     4801 attcgcgttg aatacggtat tgccatcgtc tccaaaaagt ttatcgctaa caattcagcg
     4861 tgggcgcaac atgccatcag cggtgataaa gtgttggaag gcgctctcca cccggataaa
     4921 acgaataaca ataaagatca aatgaataac aataaagatc aaatgtgcat gggtatggct
     4981 caatttaccg caaacgtaac cgaaggccgt aacagtggga tgacgaaaag agaatattat
     5041 cagcgacttg ataacatgaa cggtaaggcc gacgtcaccc agcagatgat aacgctctat
     5101 aagacttttg ttgactacgt atatgaacat ccaaagcaag acgcaaagat ttcagggacg
     5161 tatatttata ctaactgtat gaacaacttc tagaaacgta ggtgcctgaa catgatagaa
     5221 ctcagctgcc agcatagagt ggaaagcgga ggcttaccat cgtatattca tcgcgttatc
     5281 tttccgtaac tataatctgt ctcagttgat ctagtgcctt tcagggctga actaaggaat
     5341 aaagatgaat gacggtgtta tgctagattc tgacgttatt gctggtcttg agtggttagc
     5401 atcaactcaa gagaacaaac aacactttta ccagcgctta gcaaaagcac agcaatttta
     5461 catagctaca acccaaaaag ctgccaattt cgggaaacag tttgacccag aatggtatgg
     5521 ttccgacgtg gttgctggtt atttcgcaca agcaaaatcg cttatcgata atagaaggtc
     5581 ttacgaagtt actaatgctt caaatataat accatgggtt aaacaattag gagtgtgtgc
     5641 taaatcgctc gatagaatta ctggcgctag agatcgcgca atcagaatgc ttaagaatac
     5701 aacagtatta ccagacacag ccttattaga gttggttctt gctggaaact atgcatcaga
     5761 aggttatgaa gttgagttca ttccagaaca aaaagggata gcaaaaaccc cggagtttcg
     5821 ttgccgcctt ggagatggcg attatttctt tgtcgaatgc aaaaggcttc agaaaggtcc
     5881 ttacgtaaag caggaaatat tacagcatca tatacgctca catcttgccg aacttcatat
     5941 caaatccaaa aagatgaacg tatggactga tgtcacatat ttatgtgaag ttaaagacgc
     6001 gcctgaaaac tatatcatta gccacttaaa aaactttaaa ggtgtatttt atgaatggaa
     6061 tgatgattat ggaaaaggaa cgattagacc ggccaactta aatcttatca gggaagatat
     6121 cactgaaaac ggctcattac tagtcaacac taaacttgct cgtcttatca aaggctcccc
     6181 tctggaagat gaaaattacc aagttgttgc tatgggacga ccagatgagc gcgactcacg
     6241 tttcataacc aaagttaaac ttgcttcttt gataacatgg cgttgtatca atgaaaaatc
     6301 tttcgatgca cgttcgcgac atgtaacaaa aacattagca gagatagata accagctttt
     6361 ggattatggt ctaggcgtag ggcatatagc tttggatgta gatattcaaa aggatgttgc
     6421 cgataaacga agggaaaaaa actatgctgc catagtaaat tttgagcaaa aatctaaaat
     6481 agtccgcctg aatgttcatt atctcgtgcc acgcatagat gagaactctg cgtggatggt
     6541 agatgaaact gcggatgact tttttaccca tgatctggtc aaatatgtta tcccattgac
     6601 taaaatattc cccgaggctg aggtatttga gaatgaccaa cctggctggc atcaaaatta
     6661 atttattttt gatagtatga acgagggtag catttctata caaatcagtg ttcggctgat
     6721 agcgagttaa acagtttact gaaatgttct cagcaattag ctgttgccag gacctcaaaa
     6781 atccgattct ggcggaaagt gtgctgtttt accaagtatt gctcgtatct gcaaagtgtt
     6841 agagcatgtt ttaagtatat cagtaaccct tattcagtaa tgcagcaagc gtcgctcttg
     6901 aaacaccgta ttcacgcgcc aatgcgctaa cactaacccc agcggccctt ttgtctaaaa
     6961 tcgctccacg agcttccggt gcgattttag caggcctgcc caatttcctg ccttcagctt
     7021 ttgcccgtaa cagccccgca ttagtccgtt caatcagcag gtcacgctcc atctccgcaa
     7081 ccgctgctag catcgacaac aggagcttac cggcagcaga ggtgagatca gtggtgccaa
     7141 gctgatggac aataaccttg atgtttcggt cagccaacgt cctgacagtt tgcagaacgt
     7201 caattgcatc acgcccgagg cggtccagct ttgcaactac caacgtttcc ccatcgcgga
     7261 ttttgctgag catttcaaaa aacgctttgc gttgcaccgc aggaaccttt ccgctgacta
     7321 catcagaaaa ccaaaagtcg aacgtccacc cagcttgttc aagttccatg cgctggttct
     7381 cagtatcctg ctgggatgtc gaaacacgcc cgtagccaaa aatagccata gtcatctcct
     7441 catccggccc ctgccagaaa acggtgatga aaataggatg gcctgccgga aaagccaagg
     7501 ctaattttcc aacagtgtga aagcgtgcgg ttggaggatg ccggaaaacg gtcgtttgtt
     7561 ggcgcttaaa acgggtgaag acatgttagg ctgaaaccat cacttcataa ggacaaccga
     7621 aatgattaaa gacgcgattt tttcaccgtg cggtaagtat cgctactcgt tatcacgagt
     7681 ctgggacgaa tcaaaaccgt ataccctttt catagggcta aacccttcat acgccgatgc
     7741 cgagaaggat gatcgcacct tgagccgctg catatcgttt gccaaaagct ggggctacgg
     7801 tggcgtatac atggccaacc tgttcgcatt cgttcacacc cagcgacatg aaatgatgaa
     7861 agcgtctgat cccattggca aagataacga tagccacttg atacgtcttg ttagcggtgc
     7921 cggactggtt gtcgccgcat ggggaaatga aggaagacac ctcaaacgct caacgaccgt
     7981 ccgacagcta ttgccagaga gcacaatgtg cttcgtctta aacgctacag gtgagccgaa
     8041 gcaccctttg tatatgaaaa acgacagtgt tctcatacct ttaggttaac atcgataact
     8101 gcgttcagaa tatgaacgca gccatatgat atatatgact tttctttaaa atgatggtgt
     8161 gctagcttct ctcctgacac ctacctcatg cggtaaaccg gtccgggtag gtggagatca
     8221 ccgctgttta gcggaagacg tcaggatggg aactctgcgt aacgttgatt gtggtaggta
     8281 gggcgcgcaa gcgtttgatg cagccaagca gagggtgaaa gtagctatcg gcggattctt
     8341 aagtagcttc ttttgcgcat aatgcatctt tcaagaaagg tcgtcacacc acgaggaaga
     8401 cttgatcttc atctggcaag gggaaccgag caaacctaca ggctatcttc tcagatggcc
     8461 ttagggttga tatgtccggc ttcccccccg aggaagggtt tacatcttaa agaacaagtt
     8521 atgttttaac atttttaaca attacacttc tctgctgttt ggtgtcttat ggcgttcccg
     8581 tcaaaagtaa agctgccgtt tttacctcac atgcgtaaca accaaacacg aaccagaagc
     8641 tgctaacgtt caacgtgttc gcggtgtaaa tgcagccgga taagttggga gcgaaaaagt
     8701 ggatgtccta ggtttggaag cgcccctctt aagcattgca tccgataatt tccgtggcgg
     8761 tgagacagtc cttatagagg caatcagcct ccaaagttaa gcgacgcggt aaaactaaag
     8821 ctcgcccaga tttctcattg attgctcgaa ctccgctacg cttgtttcaa ctggcttcac
     8881 cagtcactct atgagagcgc cgcgaattct gcaccatgat ttcgactacc ttctttaaaa
     8941 cactcgccag agagccttac agcgcgtttg aaccgcatat cgcagcttct gcttacggat
     9001 gactgtctga ttagcagtag cttcatggcc cagtccggtt tactttattg ctatcaacta
     9061 ggagccgccc aagggcaaca cggagataac gcttgagaac cgtcgaaata ctgcaataca
     9121 ccttacgcaa aggcagcggt gccgcttttc acgccataat gcaggaaatc agtgttcccc
     9181 tacaccaaag ccacgggatc gatgttgttt cgtttggcaa ctcgcttgat gacctggatt
     9241 gctactactt gattcgcgcc tttgacagcg ctaaaagcat gactgcggtt ctcgatgctt
     9301 tttacgcaag cgcagactgg cgcagcggcc cacgcgaaga catcattggc agcatagaga
     9361 actgcattaa aacagtgatt agcctaccat cagaaagcgt gaaagggctg cggatgcaat
     9421 cgtaagtaat caagtgtatt agcttagatt tgacctaaac agctttgtgc caaaagtgga
     9481 ctaggaaaag cctagcgata aagagttgag tttaataact actaactgaa atttaatggg
     9541 tgatatatga atttagatga agaattttac gaattacaaa aactctttgc tcagaaggat
     9601 ctactgactg agaaaagacg ctctagtggc ggcggattca tggaaattct cttggtgaag
     9661 agacagaaca tgaagataaa aatatatcaa gaaaaaggac atcaacttcc ccatattcat
     9721 attgactatg gaaaacaagc gcatgctgcc agttatgcca tacaatcagg agaccgcata
     9781 gagggagatt tacctaagaa atatgatagt gatgtttcga gttggttggg gagaaatcgt
     9841 gataaaattt tagaaatctg gaatgcacta caagctggcg caccttatga accactaatc
     9901 gcagaattga ctggcggagt gtgactaact aaataatttt cccctaaaaa aattcgatgt
     9961 aggaaaatat atataagtaa gaatcatgag tgtccgctcc tcgctcaagg cggactgtta
    10021 gacttgattg tatgctgtca gagaaaaccg tcagatcaat tctacagcat ccagcccatc
    10081 aggaatgttg aatctcgcta tctcgggcaa agaaaaatca atcttttcga caacctcaac
    10141 catgtgaacc ggttttaaat ctttgctgta tcgcacttca gtttctgttt tgcctcgcac
    10201 atgcccaagt agctgcccac gcagttccga actaacaccc ttctctttaa gcaaagttga
    10261 aacagaatgc cggaaagagt ggaaagattt gcttctgtct ctttcaagtt ttaatcgacc
    10321 cgcaagcaat gattcattga accatgcgga aaccggccta ccatagcctt tggttttatg
    10381 tggtttaagc tcgggaaaaa gacggtcata cccggcagct ttcaacgcat ccacatactc
    10441 gataaaaccg agttcaatca gcgtagaatg aaccggaatc ttgcggcggg aattggcgtt
    10501 ttttagtttc tttccgtttt cgctatcgct ttcaaggttc aggtaataaa cgccgtcatc
    10561 tgccagaaca tcgtccagca ttaactgtgc aatctcgtta acccttgccc ccgtcatcaa
    10621 gcccaacaac ggcgcccaat agtgaaacgg gcaatattga tggaatcgcc cttgcgcatt
    10681 acgttcaaca gttccagcga caaaccacgg ttgcgaaaaa attgccttaa tttcatgcgg
    10741 ttcaaatatg tcagtgtgat cctgctctcg ctcacctttg tcatcgggag ggaaaaagtt
    10801 gtcgcatggc gatttcagaa acttaccatc atcaacagcc cactccattg caccataaag
    10861 gccgttgata tactttttga cagactcttc tgtcatcagt ttatcgccac attctgccgc
    10921 tttggcgatt aactcatcca gcgtcttaac cccgtgctta cctttcatca aattacggtt
    10981 agcaggaatt gtgcgcaact tgctctcata agcccggagg aaatcacggt cgaaatcttc
    11041 caactgaata tcccccatca cttcaatcat caaatcgaag taatagcgaa tatcgcggag
    11101 cgccgcttct gataccttgc ccttacgtcg atgtttcaaa tactcttcac gaagccatga
    11161 caagttttca ttgtaatgtt tgcggtgaac atattcatta gcgataacag gagcgacaag
    11221 aggcacggca gggtgaatgc taactgctgg tggcgtggtc ttaaccagcc atgtaagtcg
    11281 aaggccttca gcatgaactt tgctaatcaa caggctatca gcagttatat ctacgccggg
    11341 taaatcgaag aaccaacctc cactttggtt atcaccacta gttcgaaatg cagcctctcc
    11401 ttcggatact ccagcgctca acagacaatc taaagtatgc cggggattga aggggcggag
    11461 ataaccattt gcgaagctct cgatacccag ctcaaacgcg ctattgagaa tgatgacttc
    11521 tttaggttga tccttttcat catcagactg tttttctcgt tttgcacccg gttcccgctc
    11581 tactgcatta aattcatcaa catagaaacc gaactgacca gtcgccagcc agaataccgg
    11641 gaggttacga ttaaccacct ctcgaatcaa ttgcgatata ggcaactcga ttgactgagc
    11701 aaagttagaa agcgagatca taccctcacc agcaagcagt ggggcgcagt cacttaaagt
    11761 tcgataatcc aattgttcat actcttgtaa ggtctgatac cagaccgcca acaggagcgc
    11821 ggacttctgc tttgcttcat gaagatcgtg agtgcctgtt gaagtgtgaa tctcacactg
    11881 tcctgcgtat cgacgaaaac gttggggaac gctaatgcgg acaacataaa tgccgctggt
    11941 tcgacgatag agtttatatc gctgagacat cgcctcgtat acccttttgt aagaggggct
    12001 acgcgatttc aagcaagatt atgaattaaa ttgttttaaa acaatagttt agatgagtct
    12061 tggtgccgaa ggccggactc gaaccggcac gtatttctac ggttgatttt gaatcaactg
    12121 cgtctaccga tttcgccact tcggcactga agaggtatgc ggaaaacgtt gtggattata
    12181 cctgtcgcac gccaccatgc aagcgccagc ttgctcgcac ctcgctaagt gctgaaattt
    12241 tcagcattgc atacagcgat gtgtaacctt tgtcacactc caggcacccc gccctgccat
    12301 gctctacact tcccaaacaa caccagagaa ggaccaaaaa atgtcgatga taaaaagcta
    12361 tgccgcaaaa gaagcgggcg gcgaactgga agtttatgag tacgatcccg gtgagctgag
    12421 gccacaagat gttgaagtgc aggtggatta ttgcgggatc tgccattccg atctatcgat
    12481 gatcgacaat gaatggggat tttcacaata tccgctggtt gccgggcatg aggtgattgg
    12541 gcgcgtggtg gcgctcggga gcgccgcgca ggataaaggt ttgcaggtcg gccagcgtgt
    12601 cgggattggc tggacggcgc gtagctgtgg tcactgcgac gcctgtatta gcggtaatca
    12661 gatcaactgc gagcaaggtg ctgtgccgac gattatgaat cgcggtggct ttgccgagaa
    12721 gttgcgtgcg gactggcaat gggtgattcc actgccagaa aatattgata tcgagtccgc
    12781 cgggccgctg ttgtgcggcg gtatcacggt ctttaaacca ctgttgatgc accatatcac
    12841 tgctaccagc cgcgttgggg taattggtat tggcgggctg gggcatatcg ctataaaact
    12901 tctgcacgca atgggatgcg aggtgacggc ctttagttct aatccggcga aagagcagga
    12961 agtactggcg atgggtgccg ataaagtggt gaatagccgc gatccgcagg cactgaaagc
    13021 cctgtcgggg cagtttgatc tcattatcaa tactgtgaac gtcagcctcg actggcagcc
    13081 ttattttgag gcgctgacct atggcggtaa tttccatacg gtcggtgcgg ttctcacgcc
    13141 gttgccggtt ccggccttta cgctgattgc gggcgatcgc agtgtctctg gctctgctac
    13201 cggtacacct tatgagttgc gtaagctgat gcgctttgcc gcccgcagca aggttgcgcc
    13261 gactaccgaa ttgttcccga tgtcgaaaat taacgatgcc atcaagcatg tgcgcgacgg
    13321 taaggcgcgt taccgcgtgg ttctgaaagc tgacttctga tctctttgcc gggtgcctgt
    13381 cgcccggcac ttaaatctct ttggaaggcg tctcgcaatc tgctgaattt agcggtgaag
    13441 agtgcgcagc cgttgcctat actccaggca ggaaactgga ggatatctca tgagcgaacc
    13501 cctgttaatt gcccgcacgc cggacacaga actattttta ctgccgggaa tggctaaccg
    13561 tcacgggctg attactggcg caacggggac gggtaaaact gtcacgctgc aaaaactggc
    13621 ggagtcattg tcggaaatcg gcgtgccggt gtttatggct gatgtgaaag gcgatctgac
    13681 cggtatcgcg caggcaggaa cggcgtcgga aaaactgctc gcaaggctta aaaatatcga
    13741 cgtcaatgac tggcaaccgc atgctaatcc ggtggtggtg tgggatatct ttggcgagaa
    13801 aggccattcg gtacgggcga cggtttcaga cctggggcca ctgttgctgg tgcggctgtt
    13861 gaatctcaac gatgtgcaat ctggcgtgct gaatatcatc ttccgcattg ctgacgatca
    13921 ggggctgtta ctgctcgact ttaaagatct gcgggcgatt acccagtaca tcggcgataa
    13981 cgccaaatct ttccagaatc agtacggaaa tatcagtagc gcatcggttg gtgccatcca
    14041 gcgcgggctg ttgtcgctgg aacagcaagg cgcagcacac ttctttggtg agccgatgct
    14101 ggatatcaaa gactggatgc gcaccgatgc caacggtaaa ggcgttatca atatcctcag
    14161 cgccgagaaa ctttatcaga tgccgaaact gtacgccgcc agcctgctgt ggatgctctc
    14221 agagttgtat gaacaattgc cggaagcggg cgatctggag aaaccaaaac tggtgttttt
    14281 cttcgacgaa gcacatctgc tgtttaacga tgcaccgcag gtactgctgg ataagattga
    14341 gcaggtgata aggcttattc gctcaaaagg cgtgggcgtc tggttcgttt cgcaaaaccc
    14401 gtctgatatt ccggataatg tgctcggcca gctcggtaat cgcgttcagc acgctttgcg
    14461 ggcttttacg cccaaagatc agaaagcagt aaaagctgcg gcgcaaacca tgcgggccaa
    14521 tccggcgttt gataccgaaa aggcgattca ggaactgggc accggcgagg cgttgatctc
    14581 ttttctggat gcgaaaggaa gcccttctgt ggtggagcgt gcgatggtga tcgcgccttg
    14641 ttcgcggatg gggccggtga cggaagatga gcgtaatggc ttgattaatc actctccggt
    14701 gtatggcaaa tatgaggatg aggtggaccg ggaatccgcc tatgagatgt tgcaaaaagg
    14761 ctttcaggcc agtaccgagc agcaaaataa tccccccgcg aaagggaaag aggtagcggt
    14821 ggatgacggc attcttggtg gattgaagga tattttgttc ggcactaccg gaccacgcgg
    14881 cgggaagaaa gatggtgtgg tgcaaacaat ggccaaaagc gccgctcgcc aagtgacgaa
    14941 tcagattgta cgcgggatgt tggggagttt gctggggggg agaagaaggt aagtcaggcg
    15001 agatttgtct ttgcctgatg cgacgctggc gcgtcttatc atgcccaccc cactgcaata
    15061 tattgaattt taattatttt tcaggaatga acgaagcaca ctttgctaac aataataaag
    15121 ggagctttcg ctccctttat tcgttcattc ggttacgatt ttctcattaa cagccacagg
    15181 ctgattaaga agaagctggc gcttggcaac agtgcgccga tgatcggcgg gatgccataa
    15241 accaacgtca gcggaccgaa gatctggtcc agtacgtaga agacaaaacc gaaactgata
    15301 ccggtgacca cacgcacgcc catcggtacg ctacgcagtg ggccaaagat gaacgacagc
    15361 gccatcagca tcatcaccgc cacggatagc ggctggaaga ttttgctcca catgttgagc
    15421 tgataacgtc cggcatcctg accgctcgac ttcagatact tcacatagtt gtgcaaaccg
    15481 ctgatagaga gtgcatccgg gtccagcgcc accacgccca gtttgtccgg cgtgaggttg
    15541 gttttccagg tgccgctcac cgtctgcgaa ccggtaatct gtttcggatt ggtcagatca
    15601 gattcatcaa cctgcgacaa acgccagact ttatgttccg ggtcaaactt cgcagtagcg
    15661 gcatagcgta cggattgcag acgacgattc tcgttaaagg cataaatgct gatgccacct
    15721 aactcttcgt cacctttaac ccgctcaatg tagacgaagt tgttgccatc tttcgcccat
    15781 aagccttgct gggtagagag caacgagccg ccgtacatcg cctgcgcacg gtagttacgc
    15841 gccatctgct cgccctgcgg cgcgacccat tcgccaatcg ccatcgtcag caagaccagc
    15901 ggaatggcgg ttttcatcac cgacagcgcc acctgcatac gggtaaaacc agaagcctgc
    15961 atcaccacca gttcgctgcg ctgcgccagc atcccaagac caagcaacgc cccaagcaga
    16021 gccgccatcg ggaagaatat ttgcacatct ttcggcacgc tcagcaaggt atacattcct
    16081 gcgcctaacg cgtcgtaact cccctgcccg gcttttttca actgatcgac aaacttgata
    16141 atgcccgaca gcgacaccag catgaacagc gtcatcatga tggtggtgaa aatagtttta
    16201 ccgatatagc ggtcaagtac gccaaaaggt tgcatcacac cgctccttta cgcgaaaaac
    16261 tggcgcgcag gcggcggacc ggcacggtgt cccaaaggtt gagaacaatc gccaaagcca
    16321 gataaatcag gttaacggtc cacatccaca gcgtcgggtc cagcttacct ttaccgccgt
    16381 tcgatttcag ggaggtctgg atcaggaaga aaagtagata cagcagcatg gctggcagca
    16441 tcgacagtac gcgtccctga cgtgggttaa ccacgctcag cggtacgacc ataagtgcca
    16501 tcataaacac ggtgaatacc aacgtgatac gccagttcag ttctgcgcga gcacgatcgg
    16561 tgtcagtgtt ccacaatgtg cgcatgtcca tctggtcggt atcgttcggg tcgagcgcca
    16621 ccgcctggtg accaatgatc gcctgataat cctggaaatc cgtaatgcgg aaatcacgta
    16681 acagtgcagt gccttcgaag cgcgttccct ggttgagagt gacgacctgg gagccgtcgc
    16741 gcagctgggt taaatgtccg gaatcggcca ccaccacaga aggacgtgca ttaccttttg
    16801 gtcgaatttg cgcgaggaac acatctttga aatcgctgcc gtcaacgctt tcgatgaaca
    16861 gcaccgaact gccattagtc gcttgctgga attgcccttg cgccagcgcc gccatgccag
    16921 ggttcgcttt cgcttctgct aacacttcat cctgatgacg cgatgaccac ggtcccgccc
    16981 acatcacgtt aaccgccgcg acgattgccg tgaataccgc aaggatcatt gccgctttca
    17041 ccagaaccgc tttgctcagg ccgcaggcat gcattaccgt aatttcactt tcggtataca
    17101 gtttgcccag cgtcatcagc agcccgagga acaggcttaa tggcaggata agctgcgcca
    17161 tttccggcac gcccaacccg agaagggaga gcaccagatt cgccggaata tcgccgtcaa
    17221 ccgctgcgcc gaggatcctc actaactttt gacagaagaa gatcaaaagc aagatgaaga
    17281 gtatcgccag ctggcttttg agcgtctccc gcaccagata tcttatgatt atcactttaa
    17341 atacgcccgt aaaaactcgt cttttgcagg attttagctt gtttcatggc ttaaacgtca
    17401 tttattctct tgagtcgtcg aaatcgtcgc taagataatt atactcaacg gattcacctc
    17461 tcagattttg ttctgacgtg ccaatgccgt aataacgtta agattaacac gaagtcatcg
    17521 caacagcgga catgagttac gaaagcttgc aattctatct gtagccaccg ccgttgtctt
    17581 taagattcag gagcgtagtg catggagttt agtgtaaaaa gcggtagccc ggagaaacag
    17641 cggagtgcct gcatcgtcgt gggcgtcttc gaaccacgtc gcctttctcc gattgcagaa
    17701 cagctcgata aaatcagcga tgggtacatc agcgccctgc tacgtcgggg cgaactggaa
    17761 ggaaaaccgg ggcagacatt gttgctgcac catgttccga atgtactttc cgagcgaatt
    17821 ctccttattg gttgcggcaa agaacgtgag ctggatgagc gtcagtacaa gcaggttatt
    17881 cagaaaacca ttaatacgct gaatgatact ggctcaatgg aagcggtctg ctttctgact
    17941 gaactgcacg ttaaaggccg taacaactac tggaaagtgc gtcaggctgt cgagacggca
    18001 aaagagacgc tctacagttt cgatcagctg aaaacgaaca agagcgaacc gcgtcgtccg
    18061 ctgcgtaaga tggtgttcaa cgtgccgacc cgccgtgaac tgaccagcgg tgagcgcgcg
    18121 atccagcacg gtctggcgat tgccgccggg attaaagccg cgaaagatct cggtaatatg
    18181 ccgccgaata tctgtaacgc cgcttacctc gcttcacaag cgcgccagct ggctgacagc
    18241 tacagcaaga atgttatcac ccgcgttatc ggcgaacagc agatgaaaga gctggggatg
    18301 cattcctatc tggcggtcgg tcagggttcg caaaacgaat cgctgatgtc ggtgattgag
    18361 tacaaaggca acgcgtcgga agatgcacgc ccaatcgtgc tggtgggtaa aggtttaacc
    18421 ttcgactccg gcggtatctc gatcaagcct tcagaaggca tggatgagat gaagtacgat
    18481 atgtgcggtg cggcagcggt ttacggcgtg atgcggatgg tcgcggagct acaactgccg
    18541 attaacgtta tcggcgtgtt ggcaggctgc gaaaacatgc ctggcggacg agcctatcgt
    18601 ccgggcgatg tgttaaccac catgtccggt caaaccgttg aagtgctgaa taccgacgct
    18661 gaaggccgcc tggtactgtg cgacgtgtta acttacgttg agcgttttga gccggaagcg
    18721 gtgattgacg tggcgacgct gaccggtgcc tgcgtgatcg cgctgggtca tcacattacc
    18781 ggtctgatgg cgaaccataa tccgctggcc catgaactga ttgccgcgtc tgaacaatcc
    18841 ggtgaccgcg catggcgctt accgctgggt gacgagtatc aggaacaact ggagtccaat
    18901 tttgccgata tggcgaacat tggcggtcgt cctggtgggg cgattaccgc aggttgcttc
    18961 ctgtcacgct ttacccgtaa gtacaactgg gcgcacctgg atattgcagg aaccgcctgg
    19021 cgttctggta aagcaaaagg cgcaaccggt cgtccggtag cgttgctggc acagttcctg
    19081 ttgaaccgcg ctgggtttaa cggcgaagag taattgcgtc aggcaaggct gttattgccg
    19141 gatgcggcgt gaacgcctta tccgacctac acagcactga actcgtaggc ctgataagac
    19201 gcgcaagcgt cgcatcaggc aatgctgtta ttgtcggatg cggcatgaac gccttatccg
    19261 acctacacag caccgaactc gtaggcctga taagacgcgg cagcgtcgca tcaggcaatg
    19321 ctgttattgt cggatgcggc gtgagcgcct tatccgacct acacacagca ctgaactcgt
    19381 aggcctgata agacacaaca gcgtcgcatc aggcgctgcg gtgtatacct gatgcgtatt
    19441 taaatccacc acaagaagcc ccatatatga aaaacgcgac gttctacctt ctggacaatg
    19501 acaccaccgt cgatggctta agcgccgttg agcaactggt gtgtgaaatt gccgcagaac
    19561 gttggcgcag cggtaagcgc gtgctcatcg cctgtgaaga tgaaaagcag gcttaccggc
    19621 tggatgaagc cctgtgggcg cgtccggcag aaagctttgt tccgcataat ttagcgggag
    19681 aaggaccgcg cggcggtgcg ccggtggaga tcgcctggcc gcaaaaacgc agcagcagcc
    19741 cgcgcgatat attgattagt ctgcgaacaa gctttgcaga ttttgccacc gctttcacag
    19801 aagtggtaga cttcgttcct tatgaagatt ctctgaaaca actggcgcgc gaacgctata
    19861 aagcctaccg cgtggctggt ttcaacctga atacggcaac ctggaaataa tggaaaagac
    19921 atataaccca caagatatcg aacagccgct ttacgagcac tgggaaaagc agggctactt
    19981 taagcctaat ggcgatgaaa gccaggaaag tttctgcatc atgatcccgc cgccgaacgt
    20041 caccggcagt ttgcatatgg gtcacgcctt ccagcaaacc atcatggata ccatgatccg
    20101 ctatcagcgc atgcagggca aaaacaccct gtggcaggtc ggtactgacc acgccgggat
    20161 cgctacccag atggtcgttg agcgcaagat tgccgcagaa gaaggtaaaa cccgtcacga
    20221 ctacggccgc gaagctttca tcgacaaaat ctgggaatgg aaagcggaat ccggcggcac
    20281 gattacccgt cagatgcgcc gtctcggcaa ctccgtcgac tgggaacgtg aacgcttcac
    20341 gatggacgaa ggcctgtcca atgcggtgaa agaagttttc gttcgtctat ataaagaaga
    20401 cctgatttac cgtggcaaac gcctggtaaa ctgggacccg aaactgcgca ccgctatctc
    20461 tgacctggaa gtggaaaacc gcgaatcgaa aggttcgatg tggcacatcc gctatccgct
    20521 ggctgacggt gcgaaaaccg cagacggtaa agattatctg gtggtcgcga ctacccgtcc
    20581 ggaaaccctg ctgggcgata ctggcgtagc cgttaacccg gaagatccgc gttacaaaga
    20641 tctgattggc aaatatgtca ttctgccgct ggttaaccgt cgtattccga tcgttggcga
    20701 cgaacacgcc gacatggaaa aaggcaccgg ctgcgtgaaa atcactccgg cgcacgactt
    20761 taacgactat gaagtgggta aacgtcacgc gctgccgatg atcaacatcc tgacctttga
    20821 cggcgatatc cgtgaaagcg cccaggtgtt cgataccaaa ggtaacgaat ctgacgttta
    20881 ttccagcgaa atcccggcag agttccagaa actcgagcgt tttgctgcac gtaaagcggt
    20941 cgttgctgcg gttgacgcac tcggcctgct ggaagaaatt aaaccgcacg acctgacagt
    21001 tccttacggc gaccgtggcg gcgtagttat cgaaccaatg ctgaccgacc agtggtacgt
    21061 acgtgccgat gtactggcga agccggcagt tgaagcggtt gagaacggcg acattcagtt
    21121 cgtaccgaag cagtacgaaa acatgtactt ctcctggatg cgcgatattc aggactggtg
    21181 tatctctcgt cagctgtggt ggggtcaccg tatcccggca tggtacgacg aagcgggtaa
    21241 cgtttatgtt ggccgcaacg aagaagaagt gcgcaaggaa aataacctcg gtgccgacgt
    21301 tgccctgcgt caggacgaag acgttctcga cacctggttc tcttctgcgc tgtggacctt
    21361 ctctacccta ggctggccgg aaaacaccga cgcactgcgc cagttccacc caaccagcgt
    21421 gatggtatct ggtttcgaca tcatcttctt ctggattgcc cgcatgatca tgatgaccat
    21481 gcacttcatc aaagatgaaa atggcaaacc gcaggtgccg ttccacaccg tttacatgac
    21541 cggtctgatt cgtgacgacg aaggccagaa gatgtccaaa tccaagggta acgttatcga
    21601 cccgctggat atggttgacg gtatttcgct gccagaactg ctggaaaaac gtaccggcaa
    21661 tatgatgcag ccgcagctgg cggacaaaat ccgtaagcgc accgagaagc agttcccgaa
    21721 cggtattgag ccgcacggca ccgatgccct gcgcttcacc ctggcggcac tggcgtctac
    21781 cggtcgtgac atcaactggg atatgaagcg tctggaaggc taccgtaact tctgtaacaa
    21841 gctgtggaat gccagccgct ttgtgctgat gaacacggaa ggtcaggatt gcggcttcaa
    21901 cggcggcgaa atgacgctgt cgctggcgga ccgctggatt ctggcggagt tcaaccagac
    21961 catcaaagcg taccgcgaag cgctggacag cttccgcttc gatatcgccg caggcattct
    22021 gtatgagttt acctggaacc agttctgtga ctggtatctc gagctgacca agccggtaat
    22081 gaacggtggc accgaagcag aactgcgcgg tactcgccat acgctggtga ctgtactgga
    22141 aggtctgctg cgcctcgcgc atccgatcat tccgttcatc accgaaacca tctggcagcg
    22201 tgtgaaagta ctttgcggta tcactgccga caccatcatg ctgcagccgt tcccgcagta
    22261 cgatgcatct caggttgatg aagccgcact ggccgacacc gaatggctga aacaggcgat
    22321 cgttgcggta cgtaacatcc gtgcagaaat gaacatcgcg ccgggcaaac cgctggagct
    22381 gctgctgcgt ggttgcagcg cggatgcaga acgtcgcgta aatgaaaacc gtggcttcct
    22441 gcaaaccctg gcgcgtctgg aaagcatcac cgtgctgcct gccgatgaca aaggtccggt
    22501 ttccgttacg aagatcatcg acggcgcaga gctgctgatc ccgatggctg gcctcatcaa
    22561 caaagaagat gagctggcgc gtctggcgaa agaagtggcg aagatcgaag gcgaaatcag
    22621 ccgtatcgag aacaagctgg cgaatgaagg ctttgtcgcc cgcgcaccgg aagcggtcat
    22681 cgcgaaagag cgtgagaagc tggaaggcta tgcggaagcg aaagcgaagc tgattgaaca
    22741 gcaggctgtt atcgccgcgc tgtaatcaaa acacagtgat gaaaacgaag gccggagcat
    22801 gctccggcct ttttatctct tacagaaatg gtaaagaagg cataattccc cgtgaaatac
    22861 tggctaagaa gcctttatct ggttgttctt ctttattgat taaaggaagg tcatccaaat
    22921 cgcccagcaa atacgtattt tttgcctgcc agtcaatcat ccatattttc agcagtggat
    22981 aagccagacc gcccgtaatc ccggatatca ccaccaacgc acacatgcga taaagcatac
    23041 cgtggtatgt taaagttgag cgaaaacgaa tacgcccatc attcagtgac aggttgctca
    23101 taaaatgatt tcgcaacgac accgttaaat aactggtgct aacagcaatc ccaaaataat
    23161 agattaactg cgcgattatc attttacgtt gcatttccat aaattgctgt aaattctcaa
    23221 tatcatcatt tgcatacaca gaactatcat acgcatttaa tatttgatcg aagataatat
    23281 aaccagcaac agcgagaaaa ggtaataaag ctaaaaatgc gaggatggca tattttatac
    23341 aatacgcagt atctaatttc accttgaaac gatgtatacc gaaactggta ttgctcc
//
LOCUS       DBFIFM010000063        23250 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.063, whole genome shotgun sequence.
ACCESSION   DBFIFM010000063 DBFIFM010000000
VERSION     DBFIFM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.063"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..210
                     /locus_tag="N7C74_002093"
     CDS             <1..210
                     /locus_tag="N7C74_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2431961.1"
                     /translation="VFNKLWIEDKYHSRLEKRFNLMLVAALLTQKDNNLIHQLSALFE
                     FHYQQAPQQLGVIYQYYFEQAGVCH"
     gene            210..6524
                     /locus_tag="N7C74_002094"
     CDS             210..6524
                     /locus_tag="N7C74_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGU2431962.1"
                     /translation="MDVQQLHYAPLLNQLHQRAVRSVVSQLALRSKSLTHYLESQYFQ
                     PPGQEGSLLADPVFESTFGWMPADETMEDLRDKLISSPLVDALSKAPVPFRHQLTAWR
                     TILEDKKSLLVSSGTGSGKTECFMVPVLEDLIRQQKSTRQSLTGTQAIFLYPLNALIN
                     SQQERLREWTRGFKGKIRFALYNGETRHTKYEIQEDQLKVPEQALSREAIYEEPPSIM
                     VTNTTMLEYMLIRRKDAPIIEKSQGMLKYIVLDEAHSYIGSQAAELALLLRRVMQAFN
                     VGPGTDKPVQIIATSATIGEDSPEGNKELAKFVADLAGVTERDVKVVRGYRQIPRVSE
                     SLIRHEYPTSLTSLKSLSPQDLYQQLCHYRVAQQLRQALTHPGRQAVRLSELLNVARR
                     TWPDINHRELLQLLDLMARSREGELAFTPLRMHGFIRTLAGLWACSNKQCSHKAHELN
                     QSDWPFGQVWFEQRQYCDCGAPVFEVLRCSGCGSAYLSAKEEMRGDGTNWLVAQPATA
                     EVDEFALDVDVYSEDEDNDELDNNSQFDRLIASDGEKYIISLEETTAGKIDSEAQKHY
                     EINLLRPESRGEGRNNSFACVCCGDTQRKNNPLFRPLRLGAPFFLNEIIPTLLEFSPL
                     PQTREEQLGPFNGRRLLTFTDSRQGTARISARLQQDADKATLRALCYQELANITEPSA
                     KLSPAHCALLEKVISQFSVLPLKNSIDALKKAMAANEELSPTDIQALKIIEPMVSSLS
                     TEHAEAMQILQANSGNQQKKTMSWQTLSEQLVGSVDLADRMRGSFKELSGLDLTKQQF
                     ADFCLYSEFGRRPKNAWTLESLGLVAIHYPFIDKVTTCPQDWKTLLPDPDKQLAEWRN
                     LLKITLDFFIRENSAVFYENEQYPRWMGARFPVKMLQGPDKKNKGQKRDQLWPQIRDR
                     HYNRVIKLLIAVFPAIQPEQAHWKSLVNHLMIEVWDAIRPCLRQFESGYQLDIKQQAE
                     FYSPKQVWRCPYTRRALDVTLLGYSPYLPGSKEIAPEKAVLIEMPELPVRHWRLSGGG
                     EIAREERLEWLESNALIQHAREEGLWSTRSDRLALKDSWYRLEEHSAQRTPEQNQFNE
                     KQFKSGKVNVLNCSTTMEMGVDIGGMSLVAMNNVPPAPANYLQRAGRAGRRGESASAA
                     ITLCKNTAHGMEVFKDPLWAFNTTASAPRVRFGSSSIVQRHVNALVLGLFLRAEVPDA
                     TKLSCKWFFEGDESQCLRFLHWLNHQADQLADKLKRLTQGTVLMSLTATQLLTRTQAM
                     MQQVDIRWRSQLAILLENIEALKADNSAWEETPAGKAIAYQLRDYRGAYLFSKLISEA
                     FLPGHGFPVGVVNFNYLTADELEKRRAIKATQADPNEGGESFSRRIEKLPSRDLPTAL
                     REYAPGADVVLGGKVYRSSGIMLGKVLASGQELSGDHHIPWFWHCRKCGAGATSTTHP
                     VECSHCKADIQQLDVKRYLQPVGFATDIRYQAHNDVSMPAQLPWKDPRVLVPSSVWVS
                     LPDAGLGRYRFSHSGELFHFSEGEFGHGYAICLSCGRAESQTQPQRTPENLKNPEREN
                     THYLLRGGSNDRQGSNKLCHGHVHKDLWLGYSSRTDMVELQLNDDNGLLIRDEVAARS
                     LAVALREGLAHKLGIENTELGVTTQQARDINGYTGYSIFIYDNNAGGAGYAVQLIDHW
                     ADVFNYARKLLDCSCDKFCHHCLLSYDSQHYVNRLDRHHALTLLTNVRLQRLNLAPEY
                     QYFGDGSRVETNPLSLRIAQCLNSEIYDSCSLVLAGPQEQWDFAQWPLFKELLQFASS
                     GGNVELLVATPLANLTDSSRHQLSALAAMPGGRLQVKSIATAQLMQGKGRWLAQVTRE
                     GQSQQWAADDSATVAPGELWGQSASSPVVTLKGTSGKTFSGQTLSAEDLLPALPTGAV
                     RINLCEQLDGPLEGFGSRFWSLVTQQHAGWKQAFTRHKEITHVEYSDRYLNSPFTARL
                     LGEILTELVEQGMAERASLTVCVKKLDYNSRQHDALYNAWLNEEDRQQVVTTLLEEGY
                     LGPAWPGAISWLTGDNQSTEHGRELTVTFSDGSQHYVLLDMGLSYWRCIEDTFFDFAL
                     RVPQQVERLANTRARAVAPGNDLRSYIIAG"
     gene            6748..9606
                     /locus_tag="N7C74_002095"
     CDS             6748..9606
                     /locus_tag="N7C74_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGU2431963.1"
                     /translation="MNKQNYAPGMRVVIRDAEWRIRRADDSGDGGYLLTCDGISELVR
                     GKEGLFLTKLEQKVEILDPAKTHLVEDESANYQAAQLYIESQLRQRVPTDSKVHFGHL
                     AAMDSMPFQLDPTRMALAQPRQRILIADAVGLGKTLEAGILVSELIRRGRGKRILVLA
                     VKSMLTQFQKEFWSRFAIPLTRLDSAGLQKVRNRIPTNHNPFHYFDKTIISIDTLKQD
                     IEYRHHLENAWWDIIVIDEAHNVAERGTSSLRSKLAKLLAGRSDTLIMLSATPHDGKA
                     ESFASLMNMLDPTAIANPKEYEYADFADKNLVVRRFKKDVKDQMSGEFPERNIVKLTR
                     LASGAEEEAYRRLVESQFRDDDDEQAQSNKGRLFKITLEKALFSSPMACASVVANRLK
                     RLESRKDHNSQSQINELESLLLALNNIDASQFSKYQLLLDTIRKDLAWKANNTEDRLV
                     IFTESIKTLEFLEQQLRADLKLKDDQIATLRGDQGDTVLMETVEAFGKTQSPLRLLVC
                     SDVASEGINLHHLSHKMIHFDIPWSLMVFQQRNGRIDRYGQKHQPQIRYLLTEASEPQ
                     INGDMRVLEVLINKDEQAQKNIGDSSEFTGKFTQEEEEEQVAEFMMQDDGASLFDQLL
                     NSNVSESAEHDLFGEICSAVSSDASMVTETDTSLFASEQAYCERALGYLKASGQTIQY
                     ETLPDNTLSLVAPEELRRRFNQLPPEIAPENWQLYLSQDKTVITDAIARARGEQHAWP
                     DVQYLWQINPVVQWLDDKISSAFGRHQAPVIRLPYLLEPDEDHFILSGLFPNRKSHPM
                     VNPWIVVSFNRESLIGSQPFAEFLQRHPQLSNKLTNSGGKDRNHQRQQDLLEAAIAHA
                     REVFIHDRNAFETHINQQLNEHLQKLDVLRGRQLSQLELDFADNKQQLSVKQSRKEQR
                     QREIEHNFDSYIEWIEDTMTTEKEPYIQVIAVITGAEG"
     gene            9609..14543
                     /locus_tag="N7C74_002096"
     CDS             9609..14543
                     /locus_tag="N7C74_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2431964.1"
                     /translation="MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAQENAAREAE
                     RAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLLKA
                     LGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQF
                     PADTDKHKHDSLRKKANGEYRSWQDLLSTAVFTQNEPPRFVLLLGNRQLLLLDRTKWA
                     QNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSE
                     DLKYALRESIELLGNEAMHYLIDRGLANYTGNRAVDPDELSRECLRYMYRLLFLFYIE
                     ARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGY
                     NGGVKMQSDLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAM
                     SLSRPAKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKKAGEEFNEL
                     ETGYFVSKDEISKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTR
                     SLVKYALKELFKEQIDPISDPHAKADAILNLTVCEPAMGSAAFLNEAINQLAEAYLFH
                     KQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFV
                     PWFGYQLHCGNSLVGARRQVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHF
                     LLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNT
                     YRQQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYW
                     CALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHTTGDLFAEEGLLREE
                     SSLFSVAGRLKTEVLFRHLPRLAIVDALRKQHRFFHWDLEFCDLFAERGGFDLMLGNP
                     PWLKVEWQEAGVLGDYEPEFVLRKLSASKLATLRIDTFNQIPALEAAWRSEYEGCEGM
                     QNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAQKGVAGFLHPEGIYDDPKGGQLRAA
                     VYPRLRAHFQFQNELNLFVEVDHHAKFSSNIYSASPSTVGFEHISNLYAPQTIDACFE
                     HSGSGDIPGLKDEIESEGKLKVVWNTSGHRSRLISIATHELELFARLYDSEGTPAWQA
                     RLPALHAKQLVAVLEKFANQPNRLGDLQGQYFSTVMFDETYAQRDGTILRQTQFPQDS
                     SQWVLSGPHFFVGTPFYKTPRENCTLNSDYDCLDLLTLPDDYLPRTNYIPACDAQEYA
                     KRTPCVTWTELAEDEPKKVTDYYRLAIRAMLAQSGERTLISAIYPPEISHMNAVRSYC
                     YSSQNLLLEHSGMCFSLPFDFICKSTGKANLHQMLDGFSYVLFNPRQKALLYCLVLSL
                     NSVNDVYAGLWQSCYTPDFNTQRWSRDLPQLPQDFFAKLTPEWQRNCALRSDYSRRQA
                     LVEIDVLVAQALGLTLEELLTIYRVQFPVMRQYEADTWYDQNGRIIFTPSKGLVGVGL
                     PRTARKADLKNGFVFNVDSPEWTGGDCTDQAIGWDDVKHLKTGTVSVTFDDYTRSDEG
                     ERRTVTWQAPFIKPDREDDYKVAWAFFAQDKESA"
     gene            14543..20884
                     /locus_tag="N7C74_002097"
     CDS             14543..20884
                     /locus_tag="N7C74_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGU2431965.1"
                     /translation="MLPSVVSRQVADSVAAFLRAAFPLNSPLFNGENNDNVSMLEQFL
                     AQPETLLKGPYLSAQLPFRKSDLPLNFFPNLTLPFPPHAHQARAFQRLGSDAPQPTLV
                     ATGTGSGKTECFMFPLLNHCAGASQAGVKAIIIYPMNALATDQASRFAKTIASDPQLH
                     GKVTVGLFVGDSEIEPSKKMSAEKVITCKHTLRESPPDILLTNYKMLDYLLMRPGDQP
                     LWRYNQPGSLRYLVVDELHTFDGAQGSDLACLVRRLKHHIGVDNQRFACVGTSATVGD
                     ELGQLLDYAKTIFDQPFTDDAVIREDRYTAAEYLQNYAIAYSQYPGPEVASALDPQSY
                     ATPVAYLNGQIPLWFPDSDLQLPDDLESDDGREKRIALGSLLRRHSVMHVLLHDLQGG
                     ILSERECLENLQVMLAESADHARRVLQSILALIAQARLEVPETEHDRQKRLQAKKARP
                     VLPFVQLRSQLWLREMRRLVASVAKTPRLVFADDVAVEDREHYLPVIHCRDCHATGWA
                     SLRHGQSVQLETDLDGIYRQFFEKGRSIVLAFPDNNEKAVSGAHQKLCPTCLKLNRQS
                     SVQCGHCGHTELLQVLIPDMLKERKDELEFANECPYCNSKQGISILGSRAASLSAVMI
                     HQLYGSQFNEHKKLITFSDSVQDAAHRASFFTARTWPLMIRGQIASVLSEKETLPLDA
                     FAARVCQQTRERSPDDAHFAANVIAPNMQWLNDYQTLKNEGKLAENSDLPELVSLRLD
                     WEVYSEFTLRARIGRTLEATGYAVAGVDKDRFNPAITALHQQLCEELGDEMSGVSVEQ
                     VAHLALGVIWYQRQAGAVMHPAFESYRRDGTTFMMTQKERTSRYMPSIGPKTRKPAYA
                     SFEKINGFHRLIGAKSNPSWYQHWINRTLSNGNNLFISSVAETVLRRLFTALKIAGVV
                     KDFDTKGREAWGLVPSALVVSRDVVQLNCSCCREVVRAPADQGWQWRNAPCLSLRCEG
                     RYQEVDNTATWRWENMDIARVQGAEHTGLLSREDREVTEKSFYRGNQPWNINLLSATP
                     TLEMGIDVGDLSTVLLCSVPPAQANYLQRIGRAGRKDGNALNITVAEGNPHDQFFFEE
                     PLEMMQGQVQAPGVFLNATAILERQLAAFCMDNWVKTGVPESAICKNVKQMLDELEFG
                     RKSGFPYNLLRYIEQHHAEIAAQFTAIFPDLAAETRQQLLSYLQGAPGQRSLVQRIEE
                     ALKLLVEDRKSLRSRIDKLKRSIDKLENAPRDQNFDSDMRELTSERQALMALVNQINN
                     KQTLNFLTDEGLLPNYAFPEAGITLRSVLWRRKEGGEAREYQNTTFEYERPASTALAE
                     LAPLNNFYAGGHKVEIEQIDLKVSEPENWRICSHCNYSENIDQTGDQHKYCPKCGTPG
                     WADAGQKTTLLKLRQVYARASARDSQISDESDSREPAFFQRQLLVSFEKEDVSAAYAI
                     EEGEVPFGFEFLSKVTLRDINFGKMADDANELMIAGEAKKRTGFKVCLGCGMVQRPRD
                     HEPRHDLSCKYRTEPEKAKFEDYLYLYRQLESEALRILLPVSSYSNDRVVEASLGAAI
                     QLGLKHYFKGNVDHLKGVVYREPENEGESWRQYLVIYDTVPGGTGSLKELMRTPDNLL
                     KLLELAYQAIVECSCNNDTHKDGCYRCVYAYRDRGRMKYVSRDQARLLLAKILQARDS
                     IRVIDSIKNISLEAMMGSELEKRFISCLQENKNLIVSRSYAHQGAGWIINTRSEPMMS
                     WHLKAQVDLGAKEAVGIPCRPDYVLYPLMQSDAIKPVAIFLDGFAFHKNSVAEDVQKR
                     QAIRDSGNFWVWTVTWADLKEPGLKHVQDVLGLGHNPDMKQPKFYNLFHDTNFAALEA
                     SFRERNSFALLLDYLADPGGRTQLWQRMAAAHAWVWLDVKKSQDAGTKQKYAYEMQEN
                     APAWRLAELLPDEPFVFGGLLDSCNSSQQFIELASVLPQQAIKPTTSVAQMRSWLRLH
                     ICFDDRYTQDDGYEAGLNGFWRLVNLLQFLPDMTFTSRKAVHLPQESVDTKVSATPEA
                     VPETDESWAEIIEFGLLSAEEIALLQSHSLSAPTLGYELQNDEGEIIAEADLAWPSQK
                     WALIIDNQEFIPLFTTRGWHVAFGPIDENTLQHLSGGDK"
     gene            20881..22995
                     /locus_tag="N7C74_002098"
     CDS             20881..22995
                     /locus_tag="N7C74_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HGU2431966.1"
                     /translation="MTVQTPKVALSDGFLGAFARIPKAQQKKVQEFISKFRQDPTSNG
                     LNYEKIHDARSKNVHSVRIDQTYRGIVLKPEQGALYMLMWVDKHDEAYDWARRHDCSI
                     HPVTGAIQVIDISYIKPAAETVVDKPKLFAAYSAEQILALGVPPVFIDQVMALTDEAG
                     LNQLESIMPAEAWEPLHWLAEGLDYQEVLEEFNDHRDEPVDTNDFMEAIERSKRRFHV
                     VENEQELLQMLNAPLEKWRVFLHPSQRKLVESPANGPVRVLGGAGTGKTVVAMHRARW
                     LSQRLADKPGKKVLFTTFTRNLAADIRANLQRLCTREEMARIDVVNIDAWMSDQLKRH
                     GYDFRVVYDSDEGRRKCWNYALQQAPAELGLPDNFYAEEWQRVVQPNAVYTREEYLKV
                     SRVGRGTALSRIQRAKIWPVFEEFRAQMARAKLREMGDAMHEAIVLFKEKQVQLPYSS
                     IVVDEAQDIGAPAFTLIRSLVPESPNDLFIVGDGHQRIYRNKVVLGQCGINVRGRRSK
                     KLKINYRTTEETRQFAVGLLTGVKVDNLDGEADTSNDYLSLLHGEKPMITHAADFKEE
                     AATLVKQIQALLANQVRSQDICITARTKHACDRYASALNDAGIETFNLGNDSGDSDAR
                     PGVRVATMHRIKGLEFQYVFLVGINEGVVPEIKALASDDPVEQRDALFNERALLHVAA
                     TRAVKGLFVSSSGVPSPLLVAD"
BASE COUNT         5879 a         5542 c         6242 g         5587 t
ORIGIN      
        1 gtttttaata agctttggat tgaagataaa tatcactcac ggcttgaaaa acgttttaat
       61 ttgatgttag tcgcagcgct gttaacccaa aaagataata acctgataca tcaactgtct
      121 gcgctttttg aatttcacta tcagcaagcc ccacagcaat taggggtaat ctatcaatat
      181 tattttgaac aagcaggagt atgtcattga tggacgttca gcaactgcat tatgcgccac
      241 ttctaaatca gcttcatcag cgtgcggtga ggtcggtggt aagtcagttg gcattgcgga
      301 gcaagtcgtt gactcattat ctggaatcac aatattttca gcctcccggt caggaggggt
      361 cactgcttgc agacccggta ttcgaaagta cattcggctg gatgcctgcg gatgaaacaa
      421 tggaggattt gcgggacaag ctgatctcca gcccgctggt tgatgcattg agcaaagcgc
      481 cagtgccatt tcgtcatcaa cttactgcgt ggcgaacgat tctggaagac aaaaaatcct
      541 tgttagtgag cagcggtacg ggatccggga aaacagaatg ctttatggta ccggttctgg
      601 aagatcttat tcgtcagcaa aaatcgaccc gccagtcact aacagggaca caagctattt
      661 tcttgtatcc gttaaatgca ttaattaaca gccaacagga gcggcttcgc gaatggacgc
      721 gcggatttaa ggggaaaata cgcttcgcgc tatataatgg cgaaacccgg cataccaaat
      781 acgaaattca ggaagatcag ttgaaagtac ctgaacaagc tttgagtcgg gaagccattt
      841 atgaagaacc accttccatc atggtgacga ataccaccat gctggaatat atgctgattc
      901 gtcgtaaaga tgccccaatc atcgaaaagt ctcaggggat gttaaagtat atcgtcctgg
      961 atgaagccca tagttatatt ggttctcagg ctgctgagtt agcgctgctt ttacgtcgtg
     1021 ttatgcaggc tttcaatgtt ggacccggca cagataaacc tgtgcagatt atcgcgacct
     1081 ctgccactat tggtgaagac tctcctgaag gtaataagga gcttgctaaa tttgtcgctg
     1141 atctcgcggg cgttacagaa cgtgatgtca aagtggttcg cggttatcga caaataccgc
     1201 gggtatcgga gtcgctgatt cgtcatgaat accccacatc tttaacttct cttaaatccc
     1261 tgagccctca ggatctttac caacagcttt gtcattaccg tgtggcccag caattgcgtc
     1321 aggcgttaac tcatcccgga cggcaggcag tacgtttgag tgagttgctc aatgttgcca
     1381 gaagaacctg gccagatatt aatcaccgtg agctactgca actcctcgat ctaatggcaa
     1441 gatcgcgaga aggtgaactg gcttttacgc cattacgtat gcatgggttt atcagaacac
     1501 tggcgggtct atgggcatgt tcaaacaaac aatgctcaca taaagcacat gaattaaatc
     1561 agagtgactg gccatttggt caggtatggt ttgagcagcg ccaatattgt gactgtggag
     1621 cgccggtatt tgaagtttta cgttgctccg ggtgtggttc agcttatttg tctgccaaag
     1681 aggagatgcg cggtgatgga acgaactggt tggtagcgca accagccact gccgaagtgg
     1741 atgagtttgc tcttgatgta gatgtttatt ctgaagatga agacaacgac gaattagaca
     1801 ataactctca atttgatcgt ctgattgcga gcgatggtga aaaatatatc atttcactgg
     1861 aagaaacgac ggcggggaaa attgatagtg aagcgcaaaa gcactatgaa ataaacctgt
     1921 taagaccgga atctcgaggt gagggacgca ataactcctt tgcttgtgtt tgctgtggtg
     1981 acactcaacg gaaaaataat cctttattcc gtccgcttcg gctcggcgcg cccttttttt
     2041 taaatgaaat tattcctact ctgctggagt tttctccatt accgcaaaca agagaagagc
     2101 agttaggacc gtttaatggt cgacgcttgc tcacctttac ggattcgcgt cagggtaccg
     2161 ctcgcatttc agcccgctta cagcaggatg ccgataaagc gacgttgcga gctctttgtt
     2221 accaggagct ggcaaatatt acagagcctt cagcaaaact cagcccggca cactgtgcac
     2281 tgttagagaa agttattagc cagttttctg tcctcccttt aaaaaattcc atcgacgcgc
     2341 tgaaaaaagc gatggctgcg aatgaggagc ttagcccgac ggacattcag gcacttaaaa
     2401 ttattgagcc gatggtgagt tccttaagca cggagcatgc tgaagcgatg caaatacttc
     2461 aggctaactc tggcaatcag cagaagaaaa ccatgtcatg gcaaacgctg tctgaacagc
     2521 tggttggcag tgtggacctt gccgatagaa tgagaggcag ttttaaagaa ttgtctgggc
     2581 ttgatcttac taaacaacag tttgcagatt tttgtttata cagcgagttt ggtcgccgcc
     2641 ctaaaaatgc ctggaccctc gaaagtctgg gtctggtagc cattcactat ccattcattg
     2701 ataaagttac aacctgcccg caggactgga agacattatt acccgatccc gataagcaat
     2761 tagcggaatg gcgtaattta ttgaagatta ctcttgattt ctttatccgt gaaaacagtg
     2821 ccgtttttta cgaaaacgag caataccctc gctggatggg ggcacgtttc ccggtaaaaa
     2881 tgctccaggg gccagataaa aaaaacaaag ggcagaaacg tgatcaactt tggccacaaa
     2941 tacgcgatcg ccattataac cgggtcatta agctattaat cgcagtcttt ccggcaatac
     3001 aacctgagca ggctcactgg aaatcgttag ttaaccactt aatgattgag gtctgggatg
     3061 caattcgacc ctgcttacgt cagtttgagt cgggttatca gctggatatt aaacaacaag
     3121 ctgaatttta cagtccaaag caagtgtggc gttgccccta tactcgacgt gcgttagacg
     3181 tcacgttgct gggatactct ccgtatttgc cgggtagcaa agaaattgca cctgaaaaag
     3241 cagtgttgat tgaaatgcct gagcttcctg tacgtcactg gcgtttgtct ggtggtggag
     3301 aaatcgcccg agaagaacgt ctggagtggc tggaaagtaa tgcgctgatt caacatgccc
     3361 gcgaggaagg attatggtct actcgcagcg accgactggc cctgaaagat agctggtatc
     3421 gtctggagga acactctgcg cagagaacgc ctgagcagaa ccagtttaat gaaaaacaat
     3481 ttaagtcagg caaagttaat gtgctgaact gttccacgac catggagatg ggggtggata
     3541 ttggtggtat gagtttggtg gcaatgaaca acgttcctcc agcgccggca aattatttgc
     3601 aacgtgcagg tcgcgcgggg cgtcgtggag agagtgcatc tgctgccatt acgttatgta
     3661 aaaacacggc gcatggaatg gaggtattca aagaccccct ctgggcgttc aacactactg
     3721 ccagtgcgcc acgggttcgt tttgggagca gtagtattgt tcaacgacat gttaatgccc
     3781 tggttttagg tctgttttta cgagctgaag ttcctgatgc gacaaaactg agttgcaaat
     3841 ggttctttga aggtgatgag agccagtgtc ttcgttttct ccattggtta aatcaccagg
     3901 ccgatcagct ggcggataaa cttaaacgcc ttactcaggg aaccgtactg atgtcattga
     3961 cggcaaccca gctcttgacc cgcacacaag caatgatgca gcaggttgat atccgctgga
     4021 gaagccaact tgcgatactg ttggaaaata tcgaagcgtt gaaggcggat aacagcgcgt
     4081 gggaagaaac accagcaggt aaagccatcg cttatcagct tcgtgattat cgcggtgcat
     4141 atctgttttc taaactcatt agtgaagcgt tcctgccggg gcatggtttc ccggttggcg
     4201 tagtcaattt caattatctg acggcagatg agctggaaaa acgtcgcgca ataaaagcca
     4261 ctcaggctga ccctaatgaa gggggagaaa gcttttctcg tcgtattgaa aaattgccca
     4321 gccgtgattt gcctacagca ttacgcgagt atgcgcctgg tgcagatgtg gtattgggcg
     4381 gaaaagtata tcgttcctcg ggcattatgc ttggcaaggt gcttgccagc gggcaggagc
     4441 tgtcaggcga tcatcatatt ccgtggttct ggcattgccg taaatgtggt gcgggggcaa
     4501 ccagtactac ccatcctgtc gaatgtagtc actgcaaagc ggatatacag caacttgatg
     4561 tgaagcgtta tttacagcct gttggttttg cgacagatat tcgttatcag gcacataatg
     4621 atgtatccat gcctgcacaa cttccctgga aagatccacg tgttcttgta ccgtcttcgg
     4681 tgtgggtttc tttacccgat gctgggctag gacgttaccg ttttagccat agtggggaat
     4741 tgttccactt cagtgaaggg gaatttgggc acggctacgc aatttgcctc tcatgtggtc
     4801 gggcagaatc tcaaactcaa ccccagcgta cccctgaaaa tctaaagaac ccagagaggg
     4861 aaaatacgca ttacctgttg cgagggggca gtaacgatcg tcaggggagc aataaattgt
     4921 gccacggtca tgtgcataaa gatctgtggt tgggatatag cagtcgtaca gatatggttg
     4981 agctgcaact caatgatgat aatggcctgt tgatccgtga tgaggttgcc gcgcgttccc
     5041 ttgcggtcgc tttgcgtgaa gggttagcgc ataaattggg tatcgaaaat actgagctgg
     5101 gcgtgacgac gcagcaagcg cgcgatatca atggctatac cggatattcc atcttcattt
     5161 acgacaataa tgcgggtggt gcaggttatg cagtgcaatt gatcgaccat tgggctgatg
     5221 tctttaatta cgccagaaaa ttgctcgatt gcagttgcga taaattctgt caccactgct
     5281 tgctcagcta cgacagtcag cattatgtca acagattaga tcgtcaccat gcattaaccc
     5341 tgcttaccaa tgtacggttg cagcgactta acctcgcgcc agaatatcag tacttcggcg
     5401 atggtagccg ggttgaaact aaccctctga gtttacgtat tgcgcaatgc ctgaacagcg
     5461 agatttatga ctcctgctcg ctggttcttg cggggccgca ggaacagtgg gactttgccc
     5521 aatggccatt atttaaagag ttgctccagt ttgcctcctc cggtgggaat gttgagttgc
     5581 tggtggccac gccgctggca aacttaactg atagctccag acatcagctt tccgcgttgg
     5641 cggcgatgcc gggaggtcgc ttacaagtga aatccattgc cactgcgcag ctcatgcaag
     5701 gaaaggggcg ctggcttgcg caggtgactc gcgaagggca aagccaacag tgggccgcag
     5761 acgacagcgc taccgttgca cctggtgaat tatggggaca aagtgcgtct tcaccagtag
     5821 tgacacttaa aggaacgtcc ggaaaaacct tctcgggtca gaccttaagt gcagaagatt
     5881 tactgcccgc cttacccacc ggggcggtgc gcatcaatct ctgtgaacaa ctggacgggc
     5941 cgctggaagg ttttggttcg cgtttctggt cgttagtcac gcaacagcac gctggctgga
     6001 agcaggcatt tacccgccac aaagagataa cgcatgttga gtacagcgat cgttatctta
     6061 atagcccatt cacggcgcgt ttgttaggtg aaatcttaac cgagctggtt gaacagggaa
     6121 tggcggaacg cgccagcctc actgtttgcg tcaaaaaatt ggattacaac tctcgccaac
     6181 acgacgctct ctataacgcg tggcttaatg aggaagatcg tcagcaggtt gtgaccactt
     6241 tacttgaaga aggatatctg gggcctgcgt ggccgggagc aattagctgg ttaacggggg
     6301 ataaccagag tacggagcat ggacgagaac tgacagtcac cttcagcgat ggcagtcagc
     6361 attatgtcct gctcgacatg gggcttagct actggcgctg tattgaagat accttctttg
     6421 attttgccct gcgagtaccg cagcaagttg aacggctggc aaacacgcga gcgcgagctg
     6481 ttgccccggg taatgacctt aggagttata ttatcgctgg ataaataatt gctcccctgg
     6541 ggatatcttc caggggagtt gacccagctc actttttaat aatttttgtt tgaaaatcat
     6601 gcgcaattta tgcgcattcc ctcccatttt gtcactttgc cgggggcctt catgatatct
     6661 ttaaggccct cttttttatc gcagtgtgcc gcgccacatt tttcaggatg acgttaccgc
     6721 cactgcccca aatagacaga aaaaccgatg aataagcaaa attatgcacc tggtatgcgg
     6781 gtggttattc gtgatgccga atggcgtatt cgccgggcgg atgacagtgg tgatggtggg
     6841 tatttgctga cctgcgatgg tatttcagag cttgtgcgcg gtaaagaagg gttatttctg
     6901 accaaactgg aacaaaaagt agagatcctg gatcccgcaa aaacgcattt ggtggaagat
     6961 gaatccgcca attatcaagc ggcacagttg tatattgaaa gtcagttacg ccaacgcgta
     7021 ccgacagaca gtaaagtcca ttttggtcat ctggcggcga tggattccat gccgtttcag
     7081 ctcgatccta cgcggatggc gctggcacaa ccgcgtcagc ggatactcat tgccgacgct
     7141 gttggtctgg gtaaaacgct ggaagccggt attctggttt cggaactgat ccgtcgtggg
     7201 cgcggaaaac gtattctggt tctggcggtt aaatcgatgc tgacacaatt ccagaaggag
     7261 ttctggagcc gttttgcgat cccattgacc cgcctggatt ctgctggttt acaaaaggtg
     7321 cgtaaccgca tcccaaccaa ccacaacccg ttccactatt ttgacaagac cattatctcc
     7381 atcgatacgc tgaaacaaga cattgaatat cgtcaccatc tggaaaatgc ctggtgggat
     7441 attattgtca ttgatgaggc ccataacgtc gcagaacgtg gaaccagttc gttacgcagt
     7501 aaactggcaa aactgctcgc cgggcgcagc gatacgctga ttatgctttc agcaacaccg
     7561 catgatggta aggctgaaag ttttgccagc ctgatgaaca tgcttgaccc gacggcgatc
     7621 gccaacccga aagagtatga atacgccgat tttgccgata aaaatctggt tgtccgtcgc
     7681 ttcaaaaaag acgtgaaaga tcagatgtcc ggcgagttcc ccgagcgcaa tattgtcaaa
     7741 ttgactcgcc tggcatccgg tgccgaagaa gaagcatacc gtcgtctggt tgagagccag
     7801 ttccgtgatg atgacgatga acaggcccag tctaacaaag gtcgtctgtt caagataacc
     7861 ctcgaaaaag cgctattttc cagcccgatg gcgtgtgcca gcgtcgtggc gaatcgtctg
     7921 aagcgtcttg agagccgtaa agatcataat agccagagcc agatcaacga actggagtca
     7981 ttgctgttgg cgctgaataa cattgatgcc agccagttta gcaaatacca gttgctgctc
     8041 gacaccatcc gaaaagacct cgcctggaaa gccaataaca cggaagatcg cctggtgatc
     8101 ttcaccgaaa gtattaaaac gctggagttc ctcgaacagc aactgcgagc ggatctgaaa
     8161 ttgaaagatg accagatcgc cacgctgcgc ggcgatcagg gcgatacggt attgatggaa
     8221 accgtagaag cctttggtaa aacgcagtcg ccgttgcgtc ttctggtttg ttcggatgtg
     8281 gcatctgaag ggatcaacct gcatcacctt agccacaaaa tgattcactt tgatattccg
     8341 tggtcgttga tggtattcca gcaacgtaac gggcgtattg accgctatgg gcaaaaacat
     8401 cagccccaaa tccgctatct gcttaccgag gccagcgaac cacagatcaa tggcgatatg
     8461 cgcgtactgg aagtgttgat caacaaagac gaacaggcgc agaagaacat tggggacagt
     8521 tcggagttta ccggcaaatt tacccaggaa gaggaagaag agcaggttgc agagttcatg
     8581 atgcaggatg atggtgccag cctgtttgat caactgctga acagcaacgt ctcggaaagc
     8641 gccgaacacg atctgtttgg cgaaatatgc agtgcggttt ccagcgatgc ctcaatggtc
     8701 accgaaacag acaccagctt gtttgccagc gaacaggcgt actgcgaaag agcattgggt
     8761 tacctgaaag ccagtggtca aactatccag tatgaaacct tgcctgataa taccttgtcg
     8821 ctggtggcac cggaggagtt acgccgccgc ttcaaccagc taccgcctga aattgccccg
     8881 gagaactggc agctctattt aagtcaggat aaaaccgtca tcaccgacgc gattgcgcgc
     8941 gctcgcggtg agcaacatgc ctggcccgat gtgcagtatc tctggcaaat caacccggta
     9001 gtgcagtggc tggacgataa aatctcttcg gcttttggtc gtcatcaggc tccggttatc
     9061 cggctgccat acctgcttga acccgatgaa gatcacttca ttctttccgg gttattcccg
     9121 aaccgtaaat cacatccgat ggtgaacccg tggatagtgg tgagctttaa ccgtgaatcg
     9181 ctaatcggca gtcagccttt cgctgagttt ttacaacgtc atccgcagtt gagtaacaag
     9241 ctgactaaca gcggcggtaa agatcgtaat caccaacgcc agcaggattt actggaagcg
     9301 gctattgctc atgcccggga ggtattcatc catgatcgga atgcgtttga aacgcacatt
     9361 aaccagcaac tgaatgagca tctgcaaaag ctggacgttt tgcgtgggcg gcagttgagc
     9421 caacttgagc tggattttgc cgataacaaa cagcaattgt cagtcaagca gagccgtaaa
     9481 gagcagagac aacgcgaaat cgaacacaat tttgacagct atatcgaatg gattgaggac
     9541 accatgacga ctgaaaaaga accctacatt caggtaattg ctgttatcac cggagcggag
     9601 ggttaatcat ggcgctggtc ggtattaata acgaaaacga attttactct aaccactatt
     9661 tgggtgaggt tttcaccagt gatatccgcg atgtgctgga accctggata gcccaggaaa
     9721 atgcagcgcg tgaagcggag cgtgccgctc gtgaacaggg caaagacgtg gagccgggat
     9781 accgcgctcc gtggaaccag tttaacagtc tggcgactga gtttttccgc aaacttgccg
     9841 agcacgaaaa acagcgtcag atcccgcagc gtctggccga tcaacgtaat cgctggcagc
     9901 cattgttaaa ggcgctgggc tacgaaatta cgccacagat ccaaatgctg gatgacgata
     9961 caccgctgcc ggtactggcg cgttacaaca gcactgacgg tagcccgtgg ctgtggattg
    10021 ttgaagcaca cgatcaggaa gaaggaacgc tggatccgct ggcgctctcc ttactgaccg
    10081 cgcaattccc ggcggatacc gacaaacata agcacgacag cctgcgcaaa aaagccaacg
    10141 gtgaatatcg cagctggcag gatttgctct ctacggcggt cttcacccaa aatgaaccgc
    10201 cgcgttttgt gctgctgctc ggtaaccgtc agctattgtt gttggaccgt actaagtggg
    10261 cgcaaaaccg tctgctacgt tttgattttg aagagatttt aagccgtcgt gaaacggata
    10321 cgctgaaagc gactgcggtg ttgttacata aagattctct gctgccgggc agtggggcac
    10381 cttatcttga ctcgctggat gacaattcgc acaaacatgc gtttggtgtt tcggaagatc
    10441 tgaaatatgc cctgcgcgaa agcattgagt tgctgggcaa cgaagcgatg cattatctga
    10501 tcgaccgtgg cctggcaaac tatacaggta atcgtgcggt ggacccggat gaactgagcc
    10561 gcgaatgtct gcgttacatg taccgcctgc tgttcctgtt ctacattgaa gcgcgcccgg
    10621 agctgggtta tgcgccaatg accgccaaaa cctatctgca aggttacagc ctggaaacgt
    10681 tgcgcgatct ggagatgatc ccgctgacca gcgaagaaga tcgcaacggg cgctacttcc
    10741 acgacagcct gaatatgctg tttaaactgg tgcgcgaagg ctacaacggc ggcgtgaaaa
    10801 tgcagagtga cctggagagc ggcgaccgga tcaccatcca tagtcatcag ttcagcgtcc
    10861 cgcgtctgga aagtcatctg tttgatgcca acaacacgcg cattcttaac cgcgtggtat
    10921 tccgtaacga aaccctgcaa cagattatcc aggcgatgtc gttaagccgc ccggccaaag
    10981 ggcgctttaa ccgccgcgga cgtatttctt atcgccagtt gggtatcaac cagttgggtg
    11041 cggtgtatga ggcgctgctc tcctatcgcg gattcttcgc cagcgaagat ctctacgagg
    11101 tgaagaaagc cggggaagag tttaacgagc tggagacggg ttacttcgtc agtaaggatg
    11161 agattagcaa ataccacgaa gacgagaagg tctacgagaa agacggcagt ctgcgcattc
    11221 accgcaaagg cagctttatc taccgtatgg ccgggcgcga ccgtgagaaa tctgcctctt
    11281 attacacccc ggaagtgctg acccgctcac tggttaaata tgccctgaaa gaactgttta
    11341 aggagcagat tgatcccatt agcgatccgc acgccaaagc tgatgccatc ttaaacctca
    11401 ccgtgtgcga accggcgatg ggcagcgcgg cgttccttaa cgaagccatc aaccagctgg
    11461 cggaagcgta tctgttccac aagcagcagg cggaaggtcg ccgtattccg caggatcgtt
    11521 acacccagga gttacagcgg gtgaaaatgt acattgccga caacaacgtt ttcggcgtgg
    11581 acttaaaccc ggtggcggtg gaactggcgg aagtgtcgct gtggctgaac gccattagtg
    11641 gcgatgcgtt tgtaccgtgg tttggttacc agctgcactg cggtaactcg ctggtgggcg
    11701 cgcgccgtca ggtgttcaac aagagcgaac tgacctacaa aaaagccaaa gatccgagct
    11761 ggcttaacag cgagccggtc gaactggcga tgaacacgcc gcgtgaagag acgcagattt
    11821 tccacttcct gctgcccgac ggcggtatgg ctaactacag cgataaaact gttaagcagc
    11881 gttatccgga tgacttcaaa gcgctggaca gctggcgcaa agagtttatt aaaagctttg
    11941 ccgggcatga gattgctgat gtgcagcgta tcagcgaaaa ggtggaagca ctgtggaaca
    12001 cctatcgcca gcaacttaaa gcagaacgtc tgaaaaccgc cgacagctac ccggtgtggc
    12061 cggcagaaaa cagcgagcag acgcgttctt cgctgagcag taaagatgaa acctttagcg
    12121 gtcgtcttga agataacagc gcctaccaga agctgcgttg ggtaatggac tactggtgcg
    12181 cgctgtggtt ctggccgatc gacaaagccg atgagctacc ggatcgcggc acctggttgt
    12241 ttgagattga aaccctgctt gacgggattg taatcacgga aaaagtcact gaagttgcgg
    12301 agcacaccac cggcgatctg tttgccgaag aaggcctgct gcgagaagag tcttcgctgt
    12361 tttctgttgc tggtcgtctg aaaaccgagg tgttgttccg tcatttgccg cgtctggcga
    12421 ttgtcgatgc cctgagaaag cagcaccgtt tcttccactg ggatctggag ttctgcgacc
    12481 tgtttgccga gcgcggcggt tttgacctga tgctcggaaa cccgccgtgg ctgaaagtgg
    12541 aatggcagga agcgggcgtg ctgggtgatt acgagccgga atttgtgctg cgtaagctga
    12601 gcgcctcgaa gctggcaacg ttgcgtattg atacctttaa ccagatcccg gcgctggaag
    12661 cggcctggcg cagcgagtat gaaggctgtg aagggatgca aaacttcctg aatgcgcagc
    12721 agaactaccc ggtactgcgc ggggtgcaga ccaacttgta taaatgcttt ctgccgcagg
    12781 cctggcgatt aggggcgcag aaaggcgtgg caggtttcct gcacccggaa gggatttatg
    12841 atgacccgaa aggcgggcaa ttacgtgcgg cggtatatcc gaggctgagg gcgcattttc
    12901 agtttcagaa tgagttaaat ttgtttgttg aagttgatca ccatgcgaag tttagcagca
    12961 atatttattc tgctagccct agcacagtgg gatttgaaca tatatctaat ttgtatgctc
    13021 cgcaaactat tgatgcatgt tttgaacatt ctggcagtgg ggacattccc ggtctcaaag
    13081 acgagattga gagcgaggga aaattaaaag ttgtatggaa cacatctggc caccgttctc
    13141 gattaataag tatcgccact catgagctag aattatttgc tcgtctatat gacagcgaag
    13201 gaacgccagc ctggcaggca cgtttgccag ccttacatgc taaacaactt gttgctgtac
    13261 tggaaaagtt tgctaatcag ccgaatagat taggtgattt gcaggggcag tatttttcaa
    13321 cggttatgtt cgatgaaaca tatgctcaga gggatgggac aattttacgg cagactcaat
    13381 tccctcaaga ttcatcacaa tgggtactgt ctggccctca tttctttgtt gggacgccgt
    13441 tctacaagac tccgcgcgaa aactgtacgc ttaacagcga ttatgactgc ctggacttgc
    13501 taactctgcc tgacgactat ctgccgcgca ctaactacat tccggcatgt gatgcacagg
    13561 agtatgcaaa acgtactcca tgcgttacat ggactgaact ggctgaagat gaaccgaaga
    13621 aggtaacaga ttattatcgc ttagctatca gagccatgtt ggctcaatcg ggggaacgta
    13681 cactaattag tgctatttat ccgccagaaa taagtcacat gaacgcagta cgttcttact
    13741 gctatagctc acagaatctg ttactcgaac attcaggtat gtgtttttct ttaccttttg
    13801 attttatttg taaatctact ggcaaggcaa acttacatca gatgcttgat ggtttctcat
    13861 acgtattatt caatccgaga caaaaggcat tattatactg cttagtatta tcattaaatt
    13921 ctgtaaatga tgtatatgct ggcctttggc aatcctgcta caccccagac ttcaacaccc
    13981 agcgttggag ccgcgatctc ccgcagctcc cccaggattt cttcgccaaa ctgaccccag
    14041 agtggcagcg taactgcgct ttacgctctg actacagtcg tcgtcaggcg ctggtggaaa
    14101 tcgacgtatt ggtggcgcag gcgctggggt taactctcga agagctgctt accatttatc
    14161 gcgttcagtt cccggtgatg cgccagtacg aagcggatac ctggtacgat caaaacggtc
    14221 gcattatctt taccccaagc aaagggctgg tgggcgttgg cttgcctcgc accgcgcgta
    14281 aagctgacct gaaaaacggc tttgtcttta acgtcgacag cccggagtgg accggcggtg
    14341 actgcaccga tcaagctatc ggttgggatg atgtcaaaca tcttaaaacc ggtaccgtca
    14401 gcgtcacctt tgatgattat acccgcagcg acgaaggtga gcgccgtacc gtcacctggc
    14461 aggctccgtt tatcaagcca gatcgcgaag atgactacaa agtggcctgg gcgttctttg
    14521 cacaagataa ggagagcgcc tgatgttgcc gtccgttgtc agtcggcagg tcgccgacag
    14581 cgttgccgcc tttttacggg cggcgttccc gctaaacagc ccgctgttta acggtgagaa
    14641 taacgataat gtctcaatgc tggagcaatt tctggcccag ccggaaacgc tgctgaaagg
    14701 accatacctt tccgcccagt tgccgtttcg taaaagtgac ttaccgctga attttttccc
    14761 taatctgacg ctgccgttcc cgccgcacgc gcatcaggcc cgggcatttc agcgtctggg
    14821 aagcgacgcg ccacagccga cgctggtcgc aaccggtacc ggctccggta aaacagaatg
    14881 ttttatgttc ccgctgctca accactgcgc gggcgcgtca caagccgggg ttaaagcgat
    14941 tatcatctac ccgatgaacg ccctggcaac cgatcaggcc agccgtttcg ccaaaaccat
    15001 cgccagcgat ccacaactgc atggcaaggt gaccgtcggc ctgtttgtcg gcgacagcga
    15061 aatcgaaccc agcaaaaaaa tgtcggcaga aaaggtcatt acctgtaagc acacgctgcg
    15121 cgaaagcccg ccggacatcc tgttgaccaa ctacaaaatg ctcgactacc tgctgatgcg
    15181 ccctggcgat cagccgctct ggcgttacaa ccagcccggt tcattgcgtt acctggtcgt
    15241 ggatgaattg catacgtttg atggcgcgca agggtcggat ctggcctgtc tggtgcgacg
    15301 tttgaagcat cacataggtg tggataacca acgattcgcc tgcgtcggga catcagctac
    15361 cgtcggcgac gaactggggc aactgctcga ttacgcaaaa acaatctttg accagccgtt
    15421 taccgatgac gccgttattc gcgaagatcg ctacacggca gcggagtact tgcagaatta
    15481 cgccattgcg tacagtcagt atccggggcc ggaggtcgct tcggccctgg acccgcagag
    15541 ttacgctacc ccggtagcgt atcttaacgg gcaaatccct ttgtggttcc ccgacagcga
    15601 cttgcaactc ccggacgatc tggagagcga cgacgggcgt gaaaagcgta ttgcgctggg
    15661 gtcactgtta cgccgccaca gcgtgatgca tgtcttactc catgatctgc aaggcgggat
    15721 cctctctgaa cgagagtgtc tggaaaatct ccaggtcatg ctggcagaaa gcgccgacca
    15781 tgcccgacgc gtgctgcaaa gcattctggc actgatcgcg caggcacgtc tcgaagtacc
    15841 ggaaacggaa cacgatcggc aaaaacgtct ccaggcgaag aaagcgcgtc cggtgctgcc
    15901 gtttgtgcaa ctgcgctccc agttgtggct tcgtgagatg cgtcgtctgg tcgccagcgt
    15961 ggcgaaaacg ccccgactgg tgtttgccga tgatgtggcg gtggaagatc gcgaacacta
    16021 tttgccggtt atccactgcc gtgactgcca cgcaactggc tgggccagcc tgcgtcatgg
    16081 gcaaagcgtt cagttggaaa cagacctcga cggcatttat cgccagttct ttgaaaaagg
    16141 gcgctccatc gtccttgctt tcccggataa caacgaaaaa gcggtcagtg gcgcacacca
    16201 gaaactgtgt cctacctgtc tgaaactgaa ccgacagtcc agcgtccagt gtggacattg
    16261 cggtcatacg gaactgttgc aggtactgat cccggatatg ctcaaagagc gtaaggacga
    16321 gctggagttt gctaatgaat gcccgtactg caacagcaaa caggggattt cgattctggg
    16381 gtcacgggct gccagtctgt cggcggtgat gattcaccag ctttatggca gccagttcaa
    16441 cgaacataaa aagctgatta cgttttctga ctcggtacaa gacgccgcgc atcgtgccag
    16501 cttctttact gcccgtacct ggccgctgat gattcgtggg caaatcgcca gtgttcttag
    16561 cgaaaaagag acattaccgc tggatgcgtt cgcggcgcgt gtttgccaac aaacgcgtga
    16621 acgttcaccg gacgatgctc attttgcagc aaacgtcatc gcaccaaata tgcagtggct
    16681 gaatgattat cagacgctga aaaatgaagg caaactggcg gagaactccg atctgccgga
    16741 gctggtttcc ctgcgcctcg actgggaggt gtacagcgaa tttaccctgc gcgcgcgtat
    16801 cggacgtacg ctggaagcca ccggttatgc cgttgccgga gtggataaag atcgcttcaa
    16861 tccggcaatt accgccctgc atcaacagct ttgtgaagag ctgggtgatg agatgtctgg
    16921 tgtgagcgtc gagcaggtag cgcacctggc gctgggggtt atctggtatc aacgccaggc
    16981 cggggccgtt atgcatccgg cgttcgaaag ttatcgccgt gatggcacga cctttatgat
    17041 gacgcaaaaa gagcgtacct cgcgctatat gccttctatc ggtccgaaaa cgcgtaaacc
    17101 ggcttatgcg tcgtttgaaa aaattaatgg tttccatcgc ctgattggcg cgaagtctaa
    17161 ccccagttgg tatcagcatt ggatcaatcg cacgctgagt aacggcaata atctgtttat
    17221 cagtagcgtt gcggaaacag tgttacgacg cctgtttacg gcgttgaaaa tagccggagt
    17281 agtaaaagat tttgacacca aagggcgtga agcctgggga ctggtgcctt cggcgctggt
    17341 ggtgagccgt gacgtcgttc agcttaattg ctcatgttgc cgtgaggttg tccgggctcc
    17401 ggccgatcaa ggctggcagt ggcgtaatgc gccttgtctc tctttacgtt gtgaaggtcg
    17461 ttaccaggaa gtcgacaata cggcgacctg gcgttgggag aacatggaca tcgcgcgcgt
    17521 acagggcgca gaacacacgg ggctgcttag ccgtgaagat cgcgaagtca ccgaaaaatc
    17581 gttctaccgc ggcaaccaac cgtggaatat caacctgttg tcggcaacac cgacgctgga
    17641 gatgggtatt gacgttgggg atctttccac cgttttactc tgttctgtcc cacctgctca
    17701 ggccaactac ttgcagcgta ttggtcgcgc cggtcgtaaa gatggtaacg cgttaaacat
    17761 taccgtggca gaagggaacc cgcacgatca gtttttcttt gaagaaccac tggagatgat
    17821 gcagggccag gtgcaggctc ctggcgtctt ccttaatgca acggctattc tcgaacgcca
    17881 gctggcagct ttttgtatgg ataactgggt gaaaaccggt gtaccagaat ccgctatctg
    17941 taaaaacgtc aaacagatgc tggatgagct ggagttcggg cgtaaatcgg gttttccgta
    18001 taacttactg cgatatatcg aacagcatca tgcggagatt gctgcgcaat ttacggcaat
    18061 cttccccgat cttgccgcag aaactcgcca acagctgttg tcttatcttc agggcgcgcc
    18121 aggacaacgc tcgctggtgc agcgtatcga agaagcgtta aaactgctgg tggaagatcg
    18181 caaatctttg cgctcacgca tcgataaact caagcgcagt atcgataaac tggagaacgc
    18241 gccgcgcgat caaaactttg atagcgatat gcgcgagtta acttcggaac ggcaggcgct
    18301 gatggcgctg gtcaatcaaa tcaacaataa gcaaacgctg aatttcctca cggatgaagg
    18361 tctgctgcct aactacgcct tcccggaagc cggtattacg ttacgttctg tgctctggcg
    18421 gcgtaaagaa ggcggggaag cacgtgaata tcaaaacacc actttcgaat atgaacgccc
    18481 ggcatcaacg gcgctggctg agctggctcc gctgaataat ttctatgccg gtgggcataa
    18541 agttgaaatt gagcagatag acctgaaggt ctctgagccg gagaactggc gtatctgtag
    18601 ccactgtaac tacagcgaaa atatcgacca gacgggcgac cagcataaat attgcccgaa
    18661 atgtggcaca ccggggtggg ccgatgccgg acaaaaaacc acgctgctga aacttcgcca
    18721 ggtttatgcc cgcgccagcg cacgtgatag ccagatcagc gacgagagcg acagccgtga
    18781 acctgctttc ttccagcgtc agctattggt tagtttcgaa aaagaagatg tatcggcggc
    18841 atacgcgatt gaggaaggcg aagttccgtt tggttttgaa tttttaagca aagtcaccct
    18901 gcgggatatc aacttcggca aaatggcgga tgatgccaat gagttgatga ttgccggaga
    18961 agcgaagaag cgaaccgggt ttaaagtttg cctcgggtgc ggcatggtgc agcgtcctcg
    19021 cgatcatgaa ccacgtcatg atttgagctg caaatatcgc acggaaccgg aaaaggcgaa
    19081 gttcgaagat tatctctacc tctatcgcca gctggagtca gaagcgttgc gtattctgct
    19141 cccggtaagc agttacagta atgaccgtgt cgtggaggct tctcttgggg cggcgatcca
    19201 gctcggtctt aaacattact tcaaggggaa tgtcgatcac ctgaaaggcg ttgtgtaccg
    19261 cgagccggaa aatgaaggcg aatcctggcg gcagtatctg gtgatttacg ataccgtccc
    19321 gggggggact ggttctctta aagaactgat gcgcactccc gacaatttgc tgaaattact
    19381 tgaactggcc tatcaggcaa tagtggaatg cagttgcaac aatgatacgc ataaagatgg
    19441 ttgttatcgt tgcgtttacg cttaccgtga ccgtgggcgc atgaagtatg tctcccgcga
    19501 tcaggcgcgt ctcttgctgg cgaaaatttt gcaggcaaga gattcgatac gggtaattga
    19561 ttccatcaaa aacatttccc tcgaagccat gatgggcagt gagctggaaa aacggtttat
    19621 tagctgcctg caagaaaata aaaacctgat tgtcagtcgc agttacgcgc atcagggcgc
    19681 tgggtggatt attaacaccc gcagcgaacc gatgatgagc tggcatctta aagctcaggt
    19741 ggatttaggc gctaaagagg cggtggggat cccttgtcgc ccggattacg tgctatatcc
    19801 gcttatgcag tctgatgcca ttaaaccggt ggctattttc cttgatggct ttgcgttcca
    19861 caaaaatagc gtcgcagagg atgtgcaaaa acgccaggcg attcgggata gcggtaattt
    19921 ctgggtgtgg acagtcacgt gggccgatct caaagagcca gggcttaaac atgttcagga
    19981 tgtcctggga cttgggcata acccggatat gaaacagcct aagttttata acctgtttca
    20041 tgataccaac tttgcggccc ttgaagcgtc gttccgggaa cgcaacagtt ttgcactgct
    20101 gctggattat ctcgccgatc cgggcggaag aacgcagtta tggcagagaa tggctgctgc
    20161 acatgcctgg gtgtggttgg atgtgaagaa gtcacaggac gcgggaacga aacaaaagta
    20221 tgcctatgaa atgcaggaaa acgcgccagc ttggcggcta gcagagcttt tgccagacga
    20281 accgtttgtc tttggtggtt tactcgatag ctgtaactca tcgcagcaat ttattgagct
    20341 ggcttcggtg ttaccacaac aagccattaa accgacgacc agcgtcgcac aaatgcgcag
    20401 ctggctgcgt ttgcatatct gttttgacga tcgctatacg caggatgacg gttatgaagc
    20461 gggactcaat ggcttctggc gactggtaaa cctgttacag ttcctgccgg atatgacctt
    20521 taccagccgt aaagccgtgc atttaccgca ggaaagcgtt gacactaaag tttcggcaac
    20581 gccagaggca gtaccagaga ctgatgagag ttgggctgag attattgagt ttggcttatt
    20641 aagcgctgaa gagatcgcgt tgcttcagtc tcattctctc tctgcgccga ctctcggcta
    20701 cgaattgcaa aatgatgaag gtgaaattat tgcggaagcc gatctcgcct ggccatcaca
    20761 aaaatgggca ttgattattg ataatcaaga atttattcca ttattcacaa ccaggggttg
    20821 gcacgttgca ttcgggccaa ttgacgaaaa tacattgcaa catctgtccg gaggtgacaa
    20881 atgacggtgc aaacgcccaa ggtggcactt tctgatggtt ttctgggcgc attcgcgcgg
    20941 atcccaaagg cacagcaaaa aaaggtgcag gaattcatct ccaaattccg tcaggacccg
    21001 accagtaatg gacttaacta tgaaaagatc cacgatgcgc gcagtaaaaa tgtccattcg
    21061 gtgcgaatcg atcaaactta tcgcggcatt gttctgaaac ctgaacaggg tgccctgtat
    21121 atgctgatgt gggtggataa acacgacgaa gcatatgact gggcgcggcg gcatgattgt
    21181 tcaattcacc cggttaccgg cgcgattcag gtcattgata ttagctatat caaaccggca
    21241 gcggaaacag tggttgataa accgaaactc ttcgctgctt acagcgcgga acagattctg
    21301 gctctgggtg tgccgcctgt ctttattgat caggtgatgg cgcttaccga tgaagccggg
    21361 cttaatcaac ttgaaagcat aatgccagca gaagcctggg aacctctgca ctggctggca
    21421 gaagggctgg attaccagga agtgctggaa gagtttaacg atcatcgtga tgagcctgtt
    21481 gataccaacg actttatgga ggcgattgaa cgaagtaaac gtcgcttcca tgtcgttgaa
    21541 aatgaacagg agctgttgca gatgcttaat gcgcctttag aaaaatggcg tgtatttctg
    21601 catccgagcc agcgaaaatt ggtggagtcg cccgctaatg gtccggtacg agtactgggc
    21661 ggtgcaggta cagggaaaac ggttgttgcg atgcaccgtg cgcggtggtt atctcagcgt
    21721 ctggcagata aacccggtaa gaaagtactt ttcacgacct ttacgcgtaa cctggcggca
    21781 gatattcgcg ctaatctcca gcggttgtgt acgcgagaag agatggcgcg tattgacgtc
    21841 gttaacattg atgcctggat gagcgaccaa ctaaaacgcc acggttatga tttccgcgtg
    21901 gtatacgaca gcgatgaagg gcgacgcaaa tgctggaact atgccttgca gcaggcacct
    21961 gccgaactgg ggctacctga taatttctat gctgaagagt ggcaacgcgt tgtccagcca
    22021 aatgcggttt acacccggga ggaatacctc aaagtcagcc gggttggacg tggtacagcg
    22081 ttatcccgta ttcaacgggc gaaaatctgg ccagtctttg aagaattcag agcacaaatg
    22141 gcgcgtgcca aactgcgcga aatgggcgat gccatgcatg aggctattgt tctgtttaaa
    22201 gaaaagcagg tccaactacc ttatagcagt attgtggtcg atgaagctca agatatcggt
    22261 gccccggcat ttactcttat tcgcagcctg gttcccgagt cgcccaacga tctgtttatt
    22321 gtcggggatg ggcatcagcg tatttatcgc aataaggtcg ttcttggtca gtgtggtatt
    22381 aacgtccgtg gtcgtcgcag taaaaaattg aagattaact atcggaccac agaagagacg
    22441 cgtcaatttg ctgttgggct gttaactggt gtgaaggttg ataacctcga tggcgaagcg
    22501 gataccagta atgattattt gtcattactg cacggggaaa agcccatgat tacgcatgcg
    22561 gcagatttca aagaagaggc tgcaacgctc gtcaagcaaa ttcaggcatt gctggcgaat
    22621 caggttcgtt ctcaggatat ctgcattact gcgcgtacta aacatgcttg tgaccgctac
    22681 gcatcagcac tgaatgacgc cggaattgag acatttaatt taggcaatga tagcggtgac
    22741 agcgatgcac gccctggcgt gcgggttgca acgatgcacc gtattaaagg gcttgagttc
    22801 cagtatgtgt tcctggtggg gattaacgaa ggcgttgtgc cggaaataaa agcattagca
    22861 tccgatgatc ccgttgaaca gcgtgatgca ttatttaatg agcgcgcgtt actgcatgtg
    22921 gctgcaaccc gtgccgtaaa agggctgttt gtttcatcat ctggtgtccc tagtccgcta
    22981 ttagtggctg actgataata tgtccaaaac aggccaggag ccgaaacgct cctggtctat
    23041 ataaccgttc tggcaactgg cgatatttat attgcttagc tcccaacagc cagacaatat
    23101 caacttagaa attatattcg cttatgcggt ttgcatattg cacagtacaa cctgttttct
    23161 ctatatctaa cgtaacgtca ggatgtggat gatttttgga tggggtgaca cactggacaa
    23221 atatagctgg cacgaaaagg ctggcatttc
//
LOCUS       DBFIFM010000064        22573 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.064, whole genome shotgun sequence.
ACCESSION   DBFIFM010000064 DBFIFM010000000
VERSION     DBFIFM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.064"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..495
                     /locus_tag="N7C74_002099"
     CDS             <1..495
                     /locus_tag="N7C74_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HGU2431967.1"
                     /translation="SFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(530..1703)
                     /gene="ydcO"
                     /locus_tag="N7C74_002100"
                     /pseudo
     CDS             complement(530..1703)
                     /gene="ydcO"
                     /locus_tag="N7C74_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310065.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
     gene            1795..2331
                     /gene="sutR"
                     /locus_tag="N7C74_002101"
     CDS             1795..2331
                     /gene="sutR"
                     /locus_tag="N7C74_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310064.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HGU2431968.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVIISLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            2404..4365
                     /gene="rlhA"
                     /locus_tag="N7C74_002102"
     CDS             2404..4365
                     /gene="rlhA"
                     /locus_tag="N7C74_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HGU2431969.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVVKVAKDHLDV
                     AVTEPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(4457..4687)
                     /gene="yncJ"
                     /locus_tag="N7C74_002103"
     CDS             complement(4457..4687)
                     /gene="yncJ"
                     /locus_tag="N7C74_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HGU2431970.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4909..5085
                     /gene="hicA"
                     /locus_tag="N7C74_002104"
     CDS             4909..5085
                     /gene="hicA"
                     /locus_tag="N7C74_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HGU2431971.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            5131..5547
                     /gene="hicB"
                     /locus_tag="N7C74_002105"
     CDS             5131..5547
                     /gene="hicB"
                     /locus_tag="N7C74_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HGU2431972.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5626..7032
                     /gene="ydcR"
                     /locus_tag="N7C74_002106"
     CDS             5626..7032
                     /gene="ydcR"
                     /locus_tag="N7C74_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGU2431973.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKVPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWLWG
                     EREEQAVKQLGKLIQERL"
     gene            7277..8422
                     /gene="ydcS"
                     /locus_tag="N7C74_002107"
     CDS             7277..8422
                     /gene="ydcS"
                     /locus_tag="N7C74_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HGU2431974.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLQDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQTNALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASSL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            8440..9453
                     /gene="ydcT"
                     /locus_tag="N7C74_002108"
     CDS             8440..9453
                     /gene="ydcT"
                     /locus_tag="N7C74_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2431975.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFICVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            9454..10395
                     /gene="ydcU"
                     /locus_tag="N7C74_002109"
     CDS             9454..10395
                     /gene="ydcU"
                     /locus_tag="N7C74_002109"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2431976.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            10385..11179
                     /gene="ydcV"
                     /locus_tag="N7C74_002110"
     CDS             10385..11179
                     /gene="ydcV"
                     /locus_tag="N7C74_002110"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2431977.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLESGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            11201..12625
                     /gene="patD"
                     /locus_tag="N7C74_002111"
     CDS             11201..12625
                     /gene="patD"
                     /locus_tag="N7C74_002111"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HGU2431978.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTVACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(12722..12817)
                     /gene="yncL"
                     /locus_tag="N7C74_002112"
     CDS             complement(12722..12817)
                     /gene="yncL"
                     /locus_tag="N7C74_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HGU2431979.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            13012..13185
                     /gene="ortT"
                     /locus_tag="N7C74_002113"
     CDS             13012..13185
                     /gene="ortT"
                     /locus_tag="N7C74_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HGU2431980.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            13271..13504
                     /gene="ydcY"
                     /locus_tag="N7C74_002114"
     CDS             13271..13504
                     /gene="ydcY"
                     /locus_tag="N7C74_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HGU2431981.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(13505..13954)
                     /gene="ydcZ"
                     /locus_tag="N7C74_002115"
     CDS             complement(13505..13954)
                     /gene="ydcZ"
                     /locus_tag="N7C74_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU2431982.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNSLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(13951..14469)
                     /gene="mddA"
                     /locus_tag="N7C74_002116"
     CDS             complement(13951..14469)
                     /gene="mddA"
                     /locus_tag="N7C74_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HGU2431983.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            14650..15687
                     /gene="curA"
                     /locus_tag="N7C74_002117"
     CDS             14650..15687
                     /gene="curA"
                     /locus_tag="N7C74_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HGU2431984.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQSGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQREMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            15885..16550
                     /gene="mcbR"
                     /locus_tag="N7C74_002118"
     CDS             15885..16550
                     /gene="mcbR"
                     /locus_tag="N7C74_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HGU2431985.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(16586..18688)
                     /gene="pqqU"
                     /locus_tag="N7C74_002119"
     CDS             complement(16586..18688)
                     /gene="pqqU"
                     /locus_tag="N7C74_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HGU2431986.1"
                     /translation="MKIVFVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGSVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGMEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGVNIAWRFE"
     gene            18930..19991
                     /gene="yncE"
                     /locus_tag="N7C74_002120"
     CDS             18930..19991
                     /gene="yncE"
                     /locus_tag="N7C74_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HGU2431987.1"
                     /translation="MHLRHLFSLRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKDSVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(20106..21605)
                     /gene="ansP"
                     /locus_tag="N7C74_002121"
     CDS             complement(20106..21605)
                     /gene="ansP"
                     /locus_tag="N7C74_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HGU2431988.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            21872..22489
                     /gene="yncG"
                     /locus_tag="N7C74_002122"
     CDS             21872..22489
                     /gene="yncG"
                     /locus_tag="N7C74_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HGU2431989.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQVPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYVWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPTLQEILKRNEII"
BASE COUNT         5755 a         5538 c         5811 g         5469 t
ORIGIN      
        1 agttttgatc aaagcaaata tgacagcatc gtctggaacc caatcactta ttatccggta
       61 ccgaaacctt cgacccaggt agggcagaaa gttctggata aaattttgaa ctataccaac
      121 accgaaatga aagaagcgat agcgcagcgt aaaccactgg ttaccaccgc ggggccgcgt
      181 agtctgattt tccgtggggc cattaccggt gtagatacca gcaaagaagg gctgcaattc
      241 tatgaagtgg ttcctgttgc attagtggtt gcggggacgc aaatggctac aggccaccgt
      301 accatggata ctcgcttgta ttttgaaggt gagctgattg atgcagcgac taataaaccg
      361 gttatcaaag tcgttcgtca gggcgaaggt aaagacctga ataacgaaag tacgccaatg
      421 gctttcgaaa atattaaaca agttattgat gacatggcga ccgatgccac catgtttgac
      481 gttaacaaaa agtagttcca gacgcgccat cgttagatgg cgctttttat tatcggtttc
      541 tgtcagcgat taaattcaac accacgtaac aaacgcctcc ggcaattaat ccccaaaacg
      601 cagaaccaat cccgaccagc gtcaatccac ttgccgttac cagaaatgcc accaccgccg
      661 cgtcgcgctc acgctcatta tgcagcgcct gatacaaact gccgccaatg gtactcaaca
      721 gcgccagacc tgccagcatc tggatccaac ttacgggcag ggcagccatc atcccggtaa
      781 tggcactacc aaacagacct gcgagcaaat agaaaatgcc tgcaacggca gctgccagcc
      841 aacgttgatc tttatccgga tgcgcttccg ggctttggca aatagccgcg gtgattgccg
      901 caataccgac ggaataaacg ccgaaagggg aaaaaaccag tgccagcaat ccagtaaaca
      961 caatcaacgg cgaaacagga gccgaatacc cggctgcttt cattgctgcg atacccggtg
     1021 cgttttgcga tgccatcgtc accagaaaaa ggggggagtg caacgctcag gctgtgagca
     1081 aacgaaaaat caggggtaat ataagtgggg agaacgggtt taaagacaac atcagttgtg
     1141 acaacgtcac cttgcgcgat aacgatcacg atcccaataa tcatcgcggc aattaccgca
     1201 tagcgcggcg caacggcctt ggttgccagc cataccagca acatacttcc acacaacgta
     1261 aattgaccgt ccagactggc aaacgcctgt aaaccaaagc gtaataaaat cccggcaagc
     1321 attgctgccg caagcgagtg cggaataatg cgcatcagac gagcaaagag tcccgttatg
     1381 ccgcagagga ctattaacgc gttggtgaca ataaaaacgc cgatggcttc gttaagtgtt
     1441 agtccctgca atccggtgac caacaaagcc gcgccaggcg ttgaccatgc ggtgagaaca
     1501 ggtacgcgat accataatgt cagagtcagc gtactgacgc ccattgccag ccccagcgcc
     1561 gtcatccagc cagagatttg tgcagtggtg gctccggcga caatcgctgc ttgccagatt
     1621 attgccgctg aactggcgta gccaattaat accgccagaa accccgccag tagcgtgggt
     1681 ggaggaacag aaaacagacg catagtcacc tcgtgcgtta tagcgtacag gcaccgtagc
     1741 atttgtccgt tataacgcac aagtgataaa cttccgtttt gccggaggag tcgcatggaa
     1801 aatctcgctc gctttttatc caccacactt aaacaactac gtcagcagcg cggctggagt
     1861 ctttcgcgac tggcagaagc gacgggcgtt tctaaagcaa tgttaggaca gattgagcgt
     1921 aatgagtcca gcccgacggt cgcgacatta tggaaaattg ccactgggtt gaacgtgcca
     1981 ttttcgacat ttatttctcc gccacagtcc gctacgcctt cagtttatga tccacaacag
     2041 caggcgatgg tgatcatttc gctgtttcct tatgatccac aactctgctt tgaacacttc
     2101 tcgattcaga tggcacccgg tgcaataagc gaatcgacgc cccatgagaa gggggtaatc
     2161 gaacatgtcg tcgtcattga tggacaactt gatctgtgcg ttgatggtga atggcagacc
     2221 cttaattgtg gggaaggcgt tcgatttgct gcagacgtca cgcatatcta tcgtaatggt
     2281 ggggagcaaa ccgtacattt tcattccctc atccattacc cgcgcagtta agcgggaaaa
     2341 ctatttcgca acgtcgcgct tgtggctaaa atagccgcca tttttcagct actggataag
     2401 aatgtgaccg tatcttctca tcgacttgaa ctgttaagcc cggcacgcga tgccgccatt
     2461 gcccgcgaag ctattttgca cggtgccgat gctgtttata tcggcggccc tggttttggt
     2521 gcccgtcata atgccagtaa tagcctgaaa gatattgccg agctggtgcc gtttgcccat
     2581 cgttatggtg caaaaatttt cgtcacgctt aacaccattt tgcatgatga tgagctggaa
     2641 cccgcgcaac ggctgattac tgacctctac cagaccggtg tcgatgcgct gattgttcag
     2701 gatatgggga ttctggaact tgatattccg ccgattgaac tgcacgccag tacgcagtgc
     2761 gacattcgta cagtagaaaa agcgaagttc ctctctgatg ttggcttcac gcagattgtg
     2821 ctggcgcgag agctgaatct tgatcagatc cgcgcgattc accaggccac ggacgcgacc
     2881 attgaattct ttattcatgg cgcactgtgc gtggcctatt cgggtcagtg ctacatttct
     2941 catgcgcaaa cagggcgtag cgccaaccgt ggcgattgct cgcaagcgtg ccgtttgcca
     3001 tacacattga aagacgatca ggggcgggtg gtttcctatg aaaaacatct gctgtcgatg
     3061 aaagataacg atcagactgc caacctcggc gcgctgattg atgctggcgt acgttccttc
     3121 aagattgaag ggcgttacaa agatatgagc tacgtgaaga atatcaccgc ccattatcgc
     3181 cagatgcttg atgccattat tgaagaacgt ggcgatctgg cgcgcgcttc atcaggtcgt
     3241 actgaacatt tctttgttcc atcgacggaa aagactttcc accgtggtag cacagattat
     3301 tttgtgaatg cccgtaaagg cgatattggc gcgttcgatt cgccgaaatt tatcggcctg
     3361 ccggtaggtg aagtagtgaa agtggcgaaa gatcatctcg atgttgccgt taccgagcca
     3421 ctggcaaatg gcgatggcct gaacgtgttg attaaacgtg aagtcgtcgg ttttcgtgcc
     3481 aatacggtcg agaaaaccgg agaaaatcag taccgcgtct ggcccaatga aatgccagca
     3541 gatttgcaca aaattcgccc gcatcaccca ctaaaccgta atcttgatca taactggcag
     3601 caggcactga caaaaacctc tagcgaacgt cgggtggcgg tagacattga actgggcggt
     3661 tggcaggaac aattgattct gactctcacc agtgaagagg gggtcagcat aacgcatacg
     3721 ctggatgggc agttcgacga agccaataac gcagaaaaag caatgaacaa tctgaaggat
     3781 ggtctggcaa aactggggca aaccctctat tacgcccgcg atgtgcaaat taatttgccg
     3841 ggggcgctgt ttgtaccaaa cagtctgtta aaccagttcc gccgtgaagc tgccgacatg
     3901 ctggatgctg cgcgtcttgc cagttaccag cgcggcagcc gtaaaccggt tgctgatcct
     3961 gcgccggttt atccgcaaac acatctgagt ttcctcgcga acgtttacaa ccagaaagcg
     4021 cgtgaatttt atcatcgcta tggtgtgcag ctgattgacg cggcgtatga ggcacatgaa
     4081 gagaagggcg aagtcccggt gatgatcacc aagcattgtc tgcgctttgc ctttaatctg
     4141 tgcccgaaac aggcgaaagg caatatcaaa agctggaagg cgacgccaat gcaactggtt
     4201 aacggcgatg aagtattaac gctaaagttt gattgccgcc catgcgagat gcacgtcatt
     4261 ggcaaaatca aaaatcacat actgaaaatg ccgttaccgg gaagcgtagt ggcatccgta
     4321 agtccggatg agctgctgaa aacattgccg aagcgaaaag ggtaaaacgc cagttttctg
     4381 gttactcaca acttattgaa tctgcatgat attgcctgcc gggtaaggcg ttacgccgca
     4441 tccggcatca aacgactcaa cgttgtcccg cttctggttt gcgcgatttt tgccagtaaa
     4501 aatgctcgcg caaaccttcc gccgactctt ccgccacagc acgcaattca tcgctgtccg
     4561 cttcatgacg cagctgatga tccacattct ttacccacac aaattcatgt cctttgtgcc
     4621 ctgccatgag ttgtcctgag aacagcgcac acgttaataa gacaaccgat aacgccttcg
     4681 taaacatcct gccacctttt tcttaccttt tgccgctatg atgccgatcg tttcttgagg
     4741 ttattattca gttttgcaaa ttagcgcaaa gaaattctgg aatcttcctt cctgattttg
     4801 cattgcattc tgccgttgcg gcgatttagt gctattttcg agcaaattac acacggaggt
     4861 aaacactaat gtttactttg ttgatatact cagccgcagg gaggcgatgt gaaacaaagc
     4921 gagttcagac gttggctcga atctcagggc gtcgatgtag cgaatggcag caaccatttg
     4981 aaactcaggt ttcatgggag gcgcagtgtc atgccgcgtc acccctgcga tgagattaaa
     5041 gaaccattgc gtaaagcaat cctgaaacaa ctcggtttga gttaatcgcc aattaaaaag
     5101 gttaatgaca tgcgagagac agtcgaaatt atgcgttatc ccgtcactct tacacccgcg
     5161 ccggaaggcg gttatatggt ttcttttgtg gatatccctg aagcgttgac gcagggcgaa
     5221 actgtcgctg aagcgatgga agcggcaaaa gatgctttac tgaccgcatt tgatttttat
     5281 tttgaagata acgagcttat ccctttacct tcgccattaa atagtcatga tcactttatt
     5341 gaagtacctt tgagcgtcgc ctctaaggta ttgctgttaa atgctttttt acagtcagaa
     5401 atcactcagc aagagttagc caggcgaatt ggcaaaccta aacaagagat tactcgccta
     5461 tttaacttgc atcatgcgac aaaaatcgac gccgtccagc tcgcggcaaa ggcgcttggc
     5521 aaagagttat cgctggtgat ggtttaatta cagttaacga aaagttgtca tttttaacaa
     5581 ctgatataga ctgccgaatc atctgcacat aattacgatt cgataatgaa aaaataccag
     5641 cagcttgcag aacaattacg cgagcagatt gcgtcgggta tctggcaacc cggcgatcgt
     5701 ttaccttcgt tgcgtgacca ggtggcgctt tctggcatga gctttatgac tgtcagccat
     5761 gcctatcagt tgctcgaaag tcagggatat attatcgcac gaccgcagtc gggttattac
     5821 gttgcgccac aggcaataaa aatgccgaaa gtgccagtca ttccagtcac tcgagatgaa
     5881 gcagtcgata tcaacactta tatttttgat atgttgcagg ccagtcgcga tccgtcggtc
     5941 gttccgtttg cctcggcctt tcccgacccg cgacttttcc ccctccaaca actaaaccgc
     6001 tcgctggcgc aggtaagcaa aaccgccacg gcgatgagcg tgattgaaaa cttgccgcca
     6061 ggaaacgcag aactgcgtca ggctattgcc cgtcgctatg ccttacaggg gatcaccatt
     6121 tctcctgatg aaattgtcat cactgccggg gcgttagagg cattaaacct cagtttgcaa
     6181 gcggtaactg aaccgggcga ttgggtgata gtagagaatc cttgtttcta cggtgcgttg
     6241 caggcgctgg agcggctacg gctgaaggcg ttatcggtgg cgacggatgt taaagaaggg
     6301 atcgatcttc aggcgctgga actggcgttg caggagtatc cggtgaaagc gtgctggctg
     6361 atgactaata gccagaatcc actcggattt accttaacgc cgcaaaaaaa agcacaactg
     6421 gtggcgttgc tcaatcagta caacgtaacg ctgattgaag atgacgttta cagcgaactt
     6481 tattttggac gggaaaaacc gctgcctgcg aaagcgtggg atcgccacga tggcgttttg
     6541 cattgctctt cgttttcgaa atgtctggtg cctggttttc gtattggttg ggtcgccgcc
     6601 ggaaaacatg cacgtaaaat tcaacgcttg cagctgatga gtacgctttc caccagctca
     6661 ccgatgcagc ttgcgctggt ggattacctt tccacgcgcc gatacgacgc ccatcttcgt
     6721 cgcctgcgtc gccagcttgc ggaacgtaaa caacgtgcct ggcaggcact gctgcgttat
     6781 ctgcctgcgg aagtgaaaat tcatcataat gacagcggtt attttctctg gctggagctc
     6841 cccgagccgt tagatgccgg cgaattgagc ctggcggcac tgacgcatca tatcagtatt
     6901 gcgccaggta aaatgttttc taccggtgaa aactggtcac gtttttttcg ttttaatacc
     6961 gcgtggctgt ggggagaacg tgaagaacag gcggtaaaac aattaggcaa acttattcaa
     7021 gaacggctgt aatagcgttt aatttaattc ctcttagatt gggtaatatg aatttcgaat
     7081 agcagtcata tttcctaact ccttgtctat actccagaag ataaccttac agacggcata
     7141 atgcgcggta gctcacaacc tgattaaatt ttctcagggg cgaaggtgtg cctgcaagcc
     7201 gccgtctatg gttaaacaag gagatatttt tacggcacgg cggctgaaca attaattacg
     7261 acaggagtaa gaccttatga gcaagacatt tgcccgcagc agcctgtgtg cgctcagcat
     7321 gacaataatg accgctcacg ccgccgaacc gcctaccaat ttagataaac cggaagggcg
     7381 actggatatt atcgcctggc cgggatatat cgaacgcgga caaactgata aacaatacga
     7441 ctgggtaacg caattcgaaa aagagacagg ctgcgcggtg aatgttaaaa ccgccgcgac
     7501 ttccgatgag atggtcagtc tgatgaccaa agggggttac gatctggtta cggcatccgg
     7561 cgatgcctcg ctgcgtttga ttatgggtaa acgcgtgcag ccgattaata ccgccttgat
     7621 tcccaactgg aaaacgcttg atccgcgcgt ggttaaaggc gactggttta acgttggcgg
     7681 caaagtttac ggcacacctt accaatgggg gccgaacctg ctgatgtaca acactaaaac
     7741 cttcccgacg ccgccggata gctggcaagt ggtttttgtt gagcaaaatc tgcaggacgg
     7801 caagagcaat aaaggccgcg ttcaggctta tgatggccct atctacattg cggacgctgc
     7861 gttgttcgtt aaagccactc agccgcagtt gggcatcagc gatccgtatc aactcaccga
     7921 agaacagtac caggcggtgc tgaaagtgct gcgtgatcaa catagtttga tccatcgcta
     7981 ctggcatgac actaccgtgc aaatgagcga tttcaaaaac gagggtgtgg tcgcttccag
     8041 tgcctggccc tatcagacca acgccctgaa agccgaaggt cagcctgtcg ctaccgtttt
     8101 cccgaaggag ggcgttaccg gttgggctga caccaccatg ctacatagcg aagcgaaaca
     8161 tccggtttgc gcctacaaat ggatgaactg gtcattaacg ccaaaagtgc agggcgatgt
     8221 ggcggcctgg tttggctcgt taccagtcgt gccggaaggg tgtaaagcca gttcgttatt
     8281 aggcgagaaa ggttgtgaaa caaacggttt taactatttc gataaaatag ccttctggaa
     8341 aacgcctata gcagaagggg gcaagtttgt tccctacagt cgctggacgc aggattacat
     8401 tgccattatg ggcggtcgct aacttcgctg gggtgcttta tgacgtacgc agtggagttt
     8461 gacaacgtct cgcggttgta cggtgacgtg cgcgcagtag atggcgtcag tattgcgata
     8521 aaagatggtg agttcttctc tatgctgggg ccgtccggct ccggcaaaac cacctgcctg
     8581 cgcctgattg ctggcttcga acagctttcc ggcggggcta tctctatctt tggtaaaccc
     8641 gccagcaatc tgccaccgtg ggagcgggac gtgaatactg tctttcagga ctacgcgcta
     8701 tttccgcata tgtcgattct tgacaatgtc gcctatgggc tgatggtcaa aggcgtgaat
     8761 aaaaagcagc ggcacgcaat ggcgcaagag gcgctggaga aagtggcgtt ggggtttgta
     8821 cagcaacgta aaccgtcaca actttctggt ggtcagcgcc agcgggttgc tatcgccaga
     8881 gcattggtga atgaaccgcg cgtattgctg ttggatgaac cgctcggcgc actggatctc
     8941 aaattgcgtg agcagatgca gcttgagctg aaaaaactgc aacagtctct cggtatcact
     9001 tttatctgcg ttacccacga tcagggcgaa gcgttatcga tgtccgatcg tgtggcggtt
     9061 ttcaataatg gacgcattga gcaggtcgat tccccgcgcg atctctatat gcgcccgcgc
     9121 acgccgtttg ttgccgggtt cgttggtaca tcgaatgttt ttgatggact gatggcagag
     9181 aaactttgtg gcatgacggg aagcttcgcc ctgcggccgg aacatatccg cctcaacacc
     9241 cctggtgaac tgcaggccaa tggcacgatc caggcggtgc aatatcaggg cgcggcaact
     9301 cgttttgaac tgaaattgaa cggcggtgaa aaactgctgg tgagtcaggc caatatgaca
     9361 ggcgaagaac tgcctgccac gctcacgccc ggacaacagg tgatggtttc ctggtcgcgt
     9421 gatgtgatgg tgccgctggt tgaggagagg tgaatggcga tgaacgtatt gcaatcacct
     9481 tcacgtccag gtctgggtaa ggtgtccggt ttcttctggc ggaatccggg gctggggctg
     9541 tttttgctgc tgcttggccc gctaatgtgg tttggcattg tctatttcgg ctcgctgcta
     9601 acgctgttat ggcagggctt ttataccttt gacgatttca ccatgtcggt aacgccggaa
     9661 ctgacgctgg cgaacatccg tgcgctgttt aatccggcga attacgacat cattctccgc
     9721 acgctgacta tggctgtggc agtcactatc gccagcgcca ttctggcttt tccaatggcg
     9781 tggtatatgg cgcgctatac cagcgggaaa atgaaagcgt ttttttatat tgcggtaatg
     9841 ttgccgatgt gggcgagcta cattgttaaa gcctatgcct ggacgttatt gctggcaaaa
     9901 gatggcgtgg ctcagtggtt tttacaacat cttgggctgg aaccactgct gacagcgttc
     9961 cttacattac ctgcggtggg cggcaatacg ctgtcaactt ccgggctggg gcgctttctg
    10021 gtgtttctct atatctggct gccgttcatg atcctgcccg tgcaggcggc gcttgagcgt
    10081 ttgccgccgt cattgttgca ggcgtcagct gatctcggcg cacgtccacg acaaaccttt
    10141 cgctatgtgg tgttgccgct ggcgatcccg ggtattgccg ctggctctat ctttaccttc
    10201 tcactcacac tgggcgattt tatcgtcccg cagctggttg gcccgccagg atattttatc
    10261 ggcaatatgg tttattccca gcagggggcg attggcaata tgccgatggc ggcggcattc
    10321 accctggtgc cgattgttct tatcgcactg tacctggcgt tcgtgaaacg tctgggagcg
    10381 tttgatgcac tctgaacgcg caccgttttt cctcaaactg gcggcctggg gcggcgttgt
    10441 tttcctacat tttcccatcc tgataatcgc cgcctatgcg tttaacactg aagatgcggc
    10501 gtttagtttt ccaccgcagg gcctgacgct gcgctggttt agcgtggcag cacagcgtag
    10561 tgatattctt gatgccgtga cactgtcact taaagtggcg gcgctggcga cattaatggc
    10621 gctggtgtta gggacgctgg cagctgccgc gctgtggcga cgagactttt tcggcaaaaa
    10681 cgccatttcg ctgttactgc tgctgcccat tgcgctgccg ggcattgtca ctggtctggc
    10741 gttattaacc gcctttaaaa ccatcaatct ggagtcggga tttttcacca tcgtggtcgg
    10801 tcatgcgact ttttgtgtag ttgtggtgtt taacaatgtc atcgcccgtt ttcgccgcac
    10861 ctcatggagt ctggttgagg cgtcaatgga tcttggggcc aatggctggc aaaccttccg
    10921 ctacgtagtg ttgccgaatc tcagttcggc gttactggca ggaggaatgc tggcgtttgc
    10981 cttgtcgttc gatgaaatca tcgttacgac ctttacggca ggtcatgaac gaacgttacc
    11041 gttgtggttg ctcaatcagc ttgggcgacc gcgtgatgta ccggtaacta acgtggtggc
    11101 actgctggtt atgttggtaa caaccttgcc gatcctggga gcctggtggc taacccgcga
    11161 aggcgacaat ggtcaataac cactgataca ggaatatgct atgcaacata agttactgat
    11221 taacggagaa ctggttagcg gcgaagggga aaaacagcct gtctataatc cggcaacggg
    11281 ggacgtttta ctggagattg ccgaggcatc cgcagagcag gtcgatgctg ctgtgcgcgc
    11341 ggcagatgca gcatttgccg aatgggggca aaccacgccg aaagtgcgtg cggaatgtct
    11401 gctgaaactg gctgatgtta tcgaagaaaa tggtcaggtt tttgccgaac tggagtcccg
    11461 taattgtggc aaaccgctgc atagtgcgtt caatgatgaa atcccggcga ttgtcgatgt
    11521 ttttcgcttt ttcgcgggtg cggcgcgctg tctgaatggt ctggcggcag gtgaatatct
    11581 tgaaggtcat acttcgatga tccgtcgcga tccgttgggg gtcgtggctt ctatcgcacc
    11641 gtggaattat ccgctgatga tggcagcgtg gaaactggct ccggcgctgg cggcagggaa
    11701 ctgcgtagtg cttaaaccat cagaaattac cccgctgacc gcgttgaagt tggcagagct
    11761 ggcgaaagat atcttcccgg caggcgtgat taacgtactg tttggcagag gcaaaacggt
    11821 gggcgatccg ctgaccggtc atcccaaagt gcggatggtg tcgctgacgg gctctatcgc
    11881 caccggcgag cacatcatca gccataccgc gccctccatt aagcgtactc atatggaact
    11941 tggtggcaaa gcgccagtga ttgtttttga tgatgcggat attgaagcag tggtcgaagg
    12001 tgtacgtaca tttggctatt acaatgccgg acaggattgt actgtggctt gtcggatcta
    12061 cgcgcaaaaa ggcatttacg atacgctggt ggaaaaactc ggtgctgcag tggcaacgtt
    12121 aaaatctggt gcgccggatg acgagtctac ggagcttgga cctttaagct cgctggcgca
    12181 tctcgaacgc gtcagcaagg cagtagaaga ggcgaaagcg acagggcaca tcaaagtgat
    12241 cactggcggt gaaaagcgca agggtaatgg ctattactat gcgccgacgc tgctggctgg
    12301 cgcattacag gacgatgcca tcgtgcaaaa agaggtattt ggtccagtag tgagtgttac
    12361 gctcttcgac aacgaagaac aggtggtaaa ctgggcgaat gacagccagt acggacttgc
    12421 atcttcggta tggacgaaag atgtgggcag ggcgcatcgc gtcagtgcac ggctgcaata
    12481 tggttgtacc tgggtcaata cccatttcat gctggtaagt gaaatgccgc acggtgggca
    12541 gaaactttct ggttacggca aggatatgtc actttatggg ctggaggatt acaccgtcgt
    12601 ccgccacgtc atggttaaac attaaggata atattgcaga tcgtaagagt atgagatgat
    12661 cttgcgtact gtcagagcac tgatttctct gacagtacgt gctgcgtgca aaatcactac
    12721 atcaaataaa ccagccaaat ctcatagaga taagagtaaa caaaccaaca gcagcaaaga
    12781 catttatcag tactacagtt ctactggaaa cattcatttt tgccaccttt tatcattgcc
    12841 cctatgtgta tagtacgggt taagaaaatc cgcaataata ctcattctga agatggataa
    12901 gggcaagttg ctgtttgatg tttttttctc aaaccggtta aaaaacttga tgcacgtcaa
    12961 aaaatgacgc atatttgcgc gcgttttatt catctggctg gacgcccgta catgtctctc
    13021 tatcaacaca tgcttgtttt ttatgcggtt atggcagcaa tcgcatttct tatcacttgg
    13081 tttctttctc acgataagaa acgcatccgt ttcttaagcg cctttctggt gggggcaaca
    13141 tggccgatga gtttcccggt ggcgctgttg ttttcactgt tttaaatctt actcactggc
    13201 ttgacggcac cacgcagact tatatcattt ggatgaatcg ataaatttca caagtggcta
    13261 aggagaaagt atgtcgcatc tggatgaagt catcgcgcgc gtggatgccg cgattgaaga
    13321 gagcgtcatt gcccatatga acgaattatt aatcgccctg agcgatgacg cggagttaag
    13381 tcgggaagat cgctacaccc agcagcagcg tttgcgcacc gctattgctc atcacggtcg
    13441 caagcataaa gaagatatgg aagcgcgcca tgaacagtta accaaaggcg gcaccatcct
    13501 gtgattaaaa tgagcgtctg gctaccaacc aggcgccaac cacaagtaaa attgcgccac
    13561 aaattgggcc gaacaacgcc cgtagtggta cgccatgact gcggaagata tccagaacca
    13621 gaccgccaat cagctggctg gcgaccagta ctgcaatagt tgtcgccgct cccacattct
    13681 gatatccgct gatactggcg aagacaaaaa acgatcccag caagccaggt ataagtgtcc
    13741 accagcgcac gctggacacc agttcgccaa accccgccat accttgttta aaccataaaa
    13801 tagaaacaaa gagcacaatc cccaccagtg agttcagcaa catggcgatg agaatggtag
    13861 aggaggtctg ggtgatgcgc accatcagcg agttctggac taccagacca attccggcgg
    13921 caatcaaaaa ggcaagggtg agcgactgat tcatccaatc gcgtccggtt cagtgcgttc
    13981 gtcgagttgc aactgcataa atgtcagatc cagccagcgc ccaaatttag tgcccacctg
    14041 cggcatttgt gcggtgacga caaatcccag cgactggtgc agatgcagcg aggcctgatt
    14101 ttgcgattcg atcccggcga ccatgacatg cttcccgcaa tctcgcgctt catcaatcaa
    14161 tcggcttaac aatttacgcc ccagaccttt gccctgatga tcgggatgga cataaaccga
    14221 atgttccacg gtatggcgaa aaccatcgaa actacgccag tcgccaaatg aggcatatcc
    14281 cgtcactacg ccatcttctt cacttaccag caccggataa cctgctatgg tccgcgcttc
    14341 aaaccaggca atgcggttat ctgcatccac cgtttggtca ttccagatag ccgccgtata
    14401 caacacggcg tggttataaa tttccgcaat ggcagcacag tcggctttgc gggcaaaacg
    14461 gatggacatg tctggcctca ataaaataat gatgatgatg ttatcaatac tatcgaaaac
    14521 agcccgaagc aacggattcc tcgatgaaat tattgcgtgt tgcagagggg gggtgacggc
    14581 tgcaaacaaa accaatcctg cgagcctgct actattgatg gattcatgcc aaaagcacag
    14641 gagagcatta tggggcaaca aaagcagcgt aatcgacgtt gggttctggc ctcgcgtcca
    14701 catggcgcac cggttccgga gaatttccgt cttgaagaag atgatgtcgc cacaccgggt
    14761 gaaggacagg tgttactgcg cacagtttat ttgtccctgg acccgtatat gcgtggacgt
    14821 atgagcgatg agccatctta ttcaccgccg gttgatattg gcggcgtgat ggtcggcggt
    14881 acggtgagcc gtgtcgtgga gtcgaatcat cctgattatc agtctggcga ctgggtgctg
    14941 ggctacagtg gatggcaaga ctatgacata tccagtggtg atgatctggt gaaacttggc
    15001 gatcatccgc aaaatccatc gtggtcgctg ggtgtgctag ggatgccagg ctttaccgct
    15061 tatatgggcc tactggatat cggtcagcct aaagagggcg aaacgttggt ggtagctgcg
    15121 gcgacaggac cagtgggggc gacggtgggg caaatcggca aacttaaagg ttgcagagtg
    15181 gtgggggtag ccggtggcgc ggaaaaatgc cgccatgcta ccgaggtgtt aggcttcgat
    15241 gtttgtcttg atcaccacgc ggatgatttt gccgaacaac tggcgaaagc gtgcccaaaa
    15301 ggtattgata tctattatga aaacgtgggc ggtaaggtat tcgatgcggt gctaccgtta
    15361 cttaatacat ctgcgcgcat tcccgtctgc ggattagtga gcagctataa cgctacagag
    15421 ctaccacccg gtccggatcg tttacctctg ttgatggcta cagtgctgaa aaaacgtatt
    15481 cgcttgcaag gttttattat cgctcaggat tatggtcacc gcatccatga gtttcagagg
    15541 gagatggggc aatgggtgaa agaggataaa atccactacc gcgaagaaat tactgacggt
    15601 ttagagaatg cgccacagac gtttatcggc ctgctgaagg gtaaaaactt cggcaaagtg
    15661 gtgatccgcg tggcgggtga tgattaaagt ttcaacggcg gcgtaagccg ccataaaaat
    15721 ccaggcaaaa agcttatttt cagctttaat taactaacca catcattgtt ctgctgctaa
    15781 taacaacaaa ggtgagtggt ttatgattat tcactttaat acaccaggtg aattccttct
    15841 gccatgcagg cagggttgga cagaaaacac gcgaaggaat taaaatgcct ggaacggaaa
    15901 aaatgaaaca tgtcagtttg actctgcagg ttgagaacga cctgaaacat cagcttagta
    15961 ttggcgcact aaaacctggc gcacgcctga ttactaaaaa tctggcggag caattaggta
    16021 tgagtattac acctgtgcgt gaagcattat tacgtctggt ttcggtgaat gcgctttctg
    16081 tcgcaccagc gcaagcattt acagttccgg aagtggggaa acgtcaatta gacgaaatca
    16141 atcggatccg ctacgaactg gaattaatgg cagttgctct ggctgttgaa aacctcaccc
    16201 cgcaagacct ggcggaactc caggaattgc tcgagaaatt acagcaggcg caagaaaagg
    16261 gcgacatgga acaaatcatt aatgtaaaca ggctatttcg cttagcgatt tatcatcgct
    16321 caaatatgcc catcctgtgt gagatgattg agcaactgtg ggtcaggatg ggacctggtt
    16381 tacattatct ctatgaagcg attaatccag cggaattacg ggagcatata gaaaactatc
    16441 atctgttact cgctgcgtta aaagcaaaag acaaagaggg atgcagacat tgtcttgctg
    16501 aaattatgca acaaaatatt gctattttat accagcaata caatcgttaa agtaaatgcc
    16561 cggcaaatac cgggcatcaa ggtaattact caaatcgcca tgcgatattc acgccgacac
    16621 cataatttcg cccgggcgca ggttcgtaat atcgcccgtt tgactcattg acaatgacag
    16681 aaccaacgta ttctttatcg aataaattat cgacacgacc aaacaaatcc acagttaaat
    16741 tgtggtaatt gtatttatac ccggtgaata agccgacgag agtataagac ggcgctttgg
    16801 ccgtattttc atcatctgcc ataatatcgc ccatataacg cgcttccatg cctgcatacc
    16861 aaccatcttc cggtacataa cctatcgacg caaagcccat attacgggcg atccctggca
    16921 tccgattacc gttacaatcc tgttcgttgc aaacattgct gcgataggtc gcatcaagcc
    16981 aggtccagga cgcgtttacg cgaaaatctc ccgcgaaacg ttgatcccat gccagttcag
    17041 cgccttgacg acgggtcttt ccagcatttt tgtaagtcgt acgcccaccg ctactgctat
    17101 cgacaacaat ttcatcatcg gtgtcggtct ggaacaatgc gagactaagc agcccatcac
    17161 caatacgcgt tttactgccg atctcaattg tatcgttggt ggatggtttt aaaccaaagt
    17221 tcataccgct ttgcccatca gcgcgataag acagctcatt aatcgtcggc gtttcaaaac
    17281 cacgcccggc tgccagatag atattccagg catcggtcat tgcatatttt aacgatccgg
    17341 ctggtagcca tttgtgataa ctggcatcac cgctgtcatc gccgttaccc ggagtaacgt
    17401 aatggtcgtt ggaatcaaac cacacggagc tgtagcgcac gccagcatcc agcgacagtt
    17461 tttcgctcag ctgccactgc gtctgtaaat agggatcgat gttccacatc agattgcgtt
    17521 cgtcgcgacg caactcacct ttttgcccgt actccggcat gccgctattc aggcggaagt
    17581 tattgtagcc cttgcggttt tcactcatgt tttcgtagtt caggccggta gtgaacgtga
    17641 ccggaacgcc cagttcacca cggtgtgtcc agcggctgtc tattccctgg taatggcgtt
    17701 gcagggtaat cacgctgccc gcatgtgacg ggttaagttg tggtgccatg ggtattgact
    17761 ggtactgggt cgtttctcgc tctccggcat acatcatcac actcatatca tcccgcgaac
    17821 tcaggctacg ttcatagcgc aacccagcct gagtttgctt gatggttttt cgcgtgtcgt
    17881 actgttctgc acgaggcgct tgttgcggat tcgctttcca ttctgctttg gttagcccac
    17941 ctgggtcatc tgctttgata tccacgctat tgaaaatcag gcttaatttg ctggcgtcat
    18001 caatgcgtac gcccagtttg gcattagcta aatttttctg tgcgccacta tggtcacgat
    18061 agccgtgggt cgtaaaacgc gtggttgaga cggtgtaatc aacatcgcca ggctgtgtgc
    18121 cgtctcccgt tgcgcccgtt gctttcagcc catagcgcca gctgccaaaa ctgccgtagt
    18181 aactactggc ttcaatggtt ggtggctgtt gcccggtctg ggtggtgaca ttcattacac
    18241 cgccagacgc gttgccatac agggcagaga aggggccacg cagcacttcc acattttgca
    18301 cactgcttaa atcgatgttg gatgtttgcc cttgcccgtc gggcatggta gcgggaatac
    18361 cgtccacata caggcgaata ccgcgaattc cgtaagtgga gcgggagcca aatccacgaa
    18421 tcgacagctg taaatcttgc gcatagttct gccggttttg tacctgcaaa ccaggcacgc
    18481 cggtcagtga ttcggacaag ttaatgcgcg gtgttgccag gcgcatctcc tcgccatcca
    18541 ccacgcttac tgctgcaggg gtatccagtt ctgaaaccac ctgcggcgcg gcactgacaa
    18601 tcatggtctg ttcatcagcg gcaaaaacaa cgggggaaag gacaagcagg gcgggcaaaa
    18661 cggtctgtcg aacgaaaaca atcttcatga aaaaagccag gttaagaatg ggaaaacacc
    18721 gtcatggtaa tgaaattgta aatttatgga aaacgaaacg gcacgatacg ttaattaatt
    18781 gagagaattg tagtcgtaac ggcaagaaat gctccacatt tgagtaaata atgattacca
    18841 ttcccattta taacaagagc gtaacgatga ttacgcttag cgaagcattg tgaagcagca
    18901 aaaatatcgg ttcatcaaag ggagtcgtca tgcatttacg tcatctgttt tcattgcgcc
    18961 tgcgtggttc attactgtta ggttcattgc ttgttgcttc atcattcagt acgcaggccg
    19021 cagaagaaat gctgcgtaaa gcggtaggta aaggtgccta cgaaatggct tatagccagc
    19081 aagaaaacgc gctgtggctc gccacttcgc aaagccgcaa actggataaa ggcggcgtgg
    19141 tttatcgtct tgatccggtc actctggaag tgacgcaggc gatccataac gatctcaagc
    19201 cgtttggtgc caccatcaat aacacgactc agacgttgtg gtttggtaac accgtaaaca
    19261 gcgcggtcac ggcgatagat gccaaaactg gcgaggtgaa aggccgtctg gtgctggatg
    19321 atcgtaagcg cacggaagag gtgcgcccgc tgcaaccgcg tgagctggta gctgatgatg
    19381 ccacgaacac cgtttacatc agtggtattg gtaaagatag cgtgatttgg gtcgttgatg
    19441 gcgagaatat caaactgaaa accgccatcc agaacaccgg taaaatgagt accggtctgg
    19501 cactggatag caaaggcaaa cgtctttaca ccactaacgc tgacggcgaa ttgattacca
    19561 tcgacaccgc cgacaataaa atcctcagcc gtaaaaagct gctggatgac ggcaaagagc
    19621 acttctttat caacatcagc cttgataccg ccaggcagcg tgcatttatc accgattcta
    19681 aagcggcaga agtgttagtg gtcgataccc gtaatggcaa tattctggcg aaggttgcgg
    19741 caccggaatc actggctgtg ctgtttaacc cagcgcgtaa tgaagcctac gtaacgcatc
    19801 gtcaggcagg taaagtcagt gtgattgacg cgaaaagcta taaagtggtg aaaacgttcg
    19861 atacgccgac tcatccgaac agcctggcgc tgtctgccga tggcaaaacg ctgtatgtca
    19921 gtgtgaaaca aaaatccact aaacagcagg aagctaccca gccagacgat gtgattcgta
    19981 ttgcgctgta atagatatgg cgagggtgat aacggagaga cttgcctgac tttgatcacg
    20041 ccgcatgcgg cacaccttcg tcagtaatct aaatttgccc ggcacgtagc cgggcaattt
    20101 tgcaattagc tcgccgtttc aggcttaaac acaatttcct gtttttcttc atcttcctcg
    20161 acgactggcg cagtgctgtg aatttcagca acgcgtttgc gcacaccaaa ccagcctata
    20221 accagcagaa taccgataat cggcagcgcc gcgatagtat aagtcccgtt cgggtaatcg
    20281 aacgccatca gcacaaggac actcagtaaa aacagtaatg tcagccagga agtgaagggc
    20341 gcgccaggca gtttaaaact gacatccgct gctttgcctt ctttaatcgc tttacgcagg
    20401 cgcatctggc acacgatgat aaacgcccat gaagcgatga ttcccagcga cgcgaagttc
    20461 aacacaatct caaatacgcg cgacggcacc agatagttga ggaatacgcc gacgacatac
    20521 acaaccagtg tcgccagaat cccggcatac ggcacatgct gacgactcat tttcgccata
    20581 aaactcggtg cggaaccgcc catcgccatt gagcgcagaa tacgtccggt gcagtacaga
    20641 cctgaattca ggctggagag ggcagcggtc agcaccacaa tgttcataat gctgccgata
    20701 tatggcacac ccagtttaga gaaaaacgtc acgaacggac tttgccccgc ctgatacgcg
    20761 ctccacggca ataacataac cagcaacacc acggagccga cgtaaaacag gccaatacgc
    20821 caaatcacac tgttaatcgc tttaggcacc atggtctgcg gatctttaca ttctccggca
    20881 gctgtaccca ccatttcaat ggaggcaaaa gcaaacacta cgccctgaat caacaccagc
    20941 gcaggcagca aaccgtgggg gaagaagccg ccattatcgg tgattaaatg aaagccagtg
    21001 gtgttgccat ccagcggctg accactaccg aggaacaccg tacccacgac caaaaaggtc
    21061 acaatggcga gcactttaat aagcgcaaac cagaactcca tctccgcaaa ccatttcaca
    21121 ccgatcatat tcatggtgcc aacgatggta agtgcagcga gcgcaaagac ccactgcggc
    21181 acgccgccaa acgcacccca gtaatgcata tacagagcga cggcggtaat atcaacaatc
    21241 cccgtcatcg cccagttgat gaagtacatc cagccagcaa cataagcggc tttctcaccc
    21301 aaaaactcac gggcataaga gacaaaactg ccactggaag ggcggtgtag caccagctca
    21361 cccaatgcac gcagaataaa aaacgaaaac aagccacaaa ttaaataaac cagtgccagt
    21421 gctggacccg ccatttgcag tcgggctcct gcacctaaaa acaagccggt gccaatcgcg
    21481 ccgccaatgg cgatcatctg tacctggcga ttgcccatcg ctttgtgata cccctcttcg
    21541 tgggcattaa gccagcggcg tttcgcggcg tgttgatctg aagtgtcggt gtcgtgttta
    21601 ctcattgctc tccctgattg ctttaatgaa aaagtcatat aagttgccat gaacaatgtt
    21661 tattcatcct ggcgatgata gttatcacca ttattcttta tgggtataaa actatcggcg
    21721 cagcatccta cccgttatcg ataaacgatg caaaacatcc ccttacaatc ctgaagggga
    21781 ttaatacaac tgacgaaaaa atgacaaatc cttttgctgg ttaacctttg tactgtccta
    21841 catttaatct ttaaaagatt gtgaggggca tatgattaag gtgtatggcg tacccggatg
    21901 gggctctaca atcagtgagc tgatgctaac actggctgat attccttatc agttcgttga
    21961 tgtcagtggt tttgaccacg agggaacttc acgcgagtta ttgaaaaccc tgaacccgtt
    22021 gtgtcaggtt ccgacactgg cactggaaaa tgacgagatt atgacggaga cagcggcgat
    22081 tgcattgatg gttctcgatc gtcgcccgga ccttgcgcca ccggttgggc gtgccgaacg
    22141 tcagcagttt caacggttat tggtctggct ggttgctaat gtctatccaa cgttcacttt
    22201 cgccgattac cctgagcgtt gggcatctga cgcacctgaa cagttaaaga agaatgttat
    22261 tgaatatcgt aaatcgcttt atgtctggct gaattcacaa ttgaccgctg aaccttatgc
    22321 gtttggtgag caactaacgc tggtggactg ttatctttgc actatgcgca catgggggcc
    22381 tgggcatgaa tggtttcagg ataatgccac gaatatcagt gcgattgctg atgcagtatg
    22441 tcagttacca acgttacaag aaatactaaa aaggaatgaa attatttgag tgggatacca
    22501 gaagattatg ttatttatta ttcagtaact ttatccacaa tgatgtgcgt aattaattag
    22561 attcatgtta tgt
//
LOCUS       DBFIFM010000065        22261 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.065, whole genome shotgun sequence.
ACCESSION   DBFIFM010000065 DBFIFM010000000
VERSION     DBFIFM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.065"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..1336)
                     /gene="djlB"
                     /locus_tag="N7C74_002123"
     CDS             complement(<1..1336)
                     /gene="djlB"
                     /locus_tag="N7C74_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HGU2431990.1"
                     /translation="MKNCWKILNIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMNEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQIILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWHEYPSTQADALMLQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGCKPRISLTRDTEHTLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAENRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDD"
     gene            complement(1333..2040)
                     /gene="ybeR"
                     /locus_tag="N7C74_002124"
     CDS             complement(1333..2040)
                     /gene="ybeR"
                     /locus_tag="N7C74_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU2431991.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTRFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDNLPLLREFLARKEYDDSGNDMFYQLFASDDAYYATLPWQP
                     LADYPTCPETLKDMSDL"
     gene            2205..3182
                     /gene="ybeQ"
                     /locus_tag="N7C74_002125"
                     /pseudo
     CDS             2205..3182
                     /gene="ybeQ"
                     /locus_tag="N7C74_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
     gene            complement(3252..3734)
                     /gene="ybeL"
                     /locus_tag="N7C74_002126"
     CDS             complement(3252..3734)
                     /gene="ybeL"
                     /locus_tag="N7C74_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HGU2431992.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            3970..6552
                     /gene="leuS"
                     /locus_tag="N7C74_002127"
     CDS             3970..6552
                     /gene="leuS"
                     /locus_tag="N7C74_002127"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HGU2431993.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVNTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPQWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            6567..7148
                     /gene="lptE"
                     /locus_tag="N7C74_002128"
     CDS             6567..7148
                     /gene="lptE"
                     /locus_tag="N7C74_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HGU2431994.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGAVSISQDTASVFRNGQTAEYQMVM
                     TVSASVLIPGRDIYPISTKVFRSFFDNPQMALAKDNEQEMIIKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7148..8179
                     /gene="holA"
                     /locus_tag="N7C74_002129"
     CDS             7148..8179
                     /gene="holA"
                     /locus_tag="N7C74_002129"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HGU2431995.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYSQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8181..8822
                     /gene="nadD"
                     /locus_tag="N7C74_002130"
     CDS             8181..8822
                     /gene="nadD"
                     /locus_tag="N7C74_002130"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HGU2431996.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCDDLLPEPVLTYINQQGLYR"
     gene            8846..9457
                     /gene="cobC"
                     /locus_tag="N7C74_002131"
     CDS             8846..9457
                     /gene="cobC"
                     /locus_tag="N7C74_002131"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HGU2431997.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            9717..10034
                     /gene="rsfS"
                     /locus_tag="N7C74_002132"
     CDS             9717..10034
                     /gene="rsfS"
                     /locus_tag="N7C74_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HGU2431998.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10038..10505
                     /gene="rlmH"
                     /locus_tag="N7C74_002133"
     CDS             10038..10505
                     /gene="rlmH"
                     /locus_tag="N7C74_002133"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HGU2431999.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            10536..12437
                     /gene="mrdA"
                     /locus_tag="N7C74_002134"
     CDS             10536..12437
                     /gene="mrdA"
                     /locus_tag="N7C74_002134"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HGU2432000.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            12440..13552
                     /gene="mrdB"
                     /locus_tag="N7C74_002135"
                     /gene_synonym="rodA"
     CDS             12440..13552
                     /gene="mrdB"
                     /locus_tag="N7C74_002135"
                     /gene_synonym="rodA"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HGU2432001.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            13563..14651
                     /gene="rlpA"
                     /locus_tag="N7C74_002136"
     CDS             13563..14651
                     /gene="rlpA"
                     /locus_tag="N7C74_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HGU2432002.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFIQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FANKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            14791..16002
                     /gene="dacA"
                     /locus_tag="N7C74_002137"
     CDS             14791..16002
                     /gene="dacA"
                     /locus_tag="N7C74_002137"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HGU2432003.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16112..16375
                     /gene="ybeD"
                     /locus_tag="N7C74_002138"
     CDS             16112..16375
                     /gene="ybeD"
                     /locus_tag="N7C74_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HGU2432004.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            16476..17117
                     /gene="lipB"
                     /locus_tag="N7C74_002139"
     CDS             16476..17117
                     /gene="lipB"
                     /locus_tag="N7C74_002139"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HGU2432005.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17377..18330
                     /gene="ybeF"
                     /locus_tag="N7C74_002140"
     CDS             17377..18330
                     /gene="ybeF"
                     /locus_tag="N7C74_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HGU2432006.1"
                     /translation="MDSNNQIESCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRIQEMFPDRQISFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            18539..19504
                     /gene="lipA"
                     /locus_tag="N7C74_002141"
     CDS             18539..19504
                     /gene="lipA"
                     /locus_tag="N7C74_002141"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HGU2432007.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(19605..19808)
                     /gene="tatE"
                     /locus_tag="N7C74_002142"
     CDS             complement(19605..19808)
                     /gene="tatE"
                     /locus_tag="N7C74_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HGU2432008.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(19937..20725)
                     /locus_tag="N7C74_002143"
     CDS             complement(19937..20725)
                     /locus_tag="N7C74_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HGU2432009.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            20818..21201
                     /gene="crcB"
                     /locus_tag="N7C74_002144"
     CDS             20818..21201
                     /gene="crcB"
                     /locus_tag="N7C74_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HGU2432010.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21255..21464)
                     /gene="cspE"
                     /locus_tag="N7C74_002145"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21255..21464)
                     /gene="cspE"
                     /locus_tag="N7C74_002145"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HGU2432011.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(21639..22199)
                     /gene="pagP"
                     /locus_tag="N7C74_002146"
     CDS             complement(21639..22199)
                     /gene="pagP"
                     /locus_tag="N7C74_002146"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HGU2432012.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIVQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         5666 a         5588 c         5526 g         5481 t
ORIGIN      
        1 catcgtcaac gggcattggc tgcggttgcg gctccggttc aacagtttgc ttctcggtaa
       61 aattcgccgc ctgaattaat aatcgacgaa taattaatgc ttccagtgga caacgatttt
      121 ctgcatcaag acggcttacg atcggcatta atgattcttc tgtaggttgg ctggcctgca
      181 ctaattgttt ggcaactaga tataatggcc ataaggtatg ttctgtatca cgcgtgagtg
      241 agatccgcgg cttacatcct ttgtgcaaaa ccatctcgac cagtgaacgc cccagagcag
      301 agagtttttc atcgctgagt atttcgtata aatgaaaacg cgtacgggcg ctgtcgccgg
      361 ggacataaag tagcggtttt ccctggtcgt taaccagttc acgagcttca atcatcatca
      421 ccactaatgg gtaataatcg actcgatgct gacggcacca ttgcaacatt aaagcatccg
      481 cctgagtgga gggatactcg tgccagtagt cacaaagttc tgccagcagg ctttcgcctt
      541 ctccacaata gacgcgttgc gcgtattcat aataacgcgc atcttcatta tcaggttcag
      601 cttcccgcca cgaccgggcg taatccagga gttcggggat tcccaggcga cctgcactat
      661 accagcggag taaatttctg tgcagttttg catcatcggg gataatcact gcgcgatgtt
      721 gcgctaaata caatgtcagc cattctggat gcgatgacca aatccgggcg aacatttggt
      781 aaaaatcgat ggtgtcattt tgcacggcaa cgggcagatg cagaatgatc tggaagttaa
      841 aaacatttcc cttcgcaatg gcatataaaa aggattccag ttcttcttcg tctatttccc
      901 cgacgttttc ctcctgcaac caccgcaatc tttctgctaa taacaccacg cactcgaagg
      961 ataaatgggc agtgttcatg gctattgtgc acagcgacca gcgtaattca tcaatctcat
     1021 tcatcgagca ataatttaat tgctgaatga atcgctgcca ggcggagggc agaaaacgtt
     1081 cactatcaga ggcaagtaac gcacgaaagg cgagcagaat ttcatgttct gctacctcat
     1141 attcttctgg ttgccaaaca gatttagcag gcgactgcgc aatccggagc gcttcctcat
     1201 acgcctgacg aagttgttta aaaccctgcg gatcggtttc tggatgaaag gacggtaaca
     1261 gcgccagata agcgcggcgg ataatatcga catcagtcgt ttcctctatg ttgaggatct
     1321 tccagcaatt tttcataggt cgctcatatc cttaagcgtt tccgggcatg tggggtagtc
     1381 agccagtggt tgccagggta aggtcgcgta atatgcatcg tcactggcaa ataattgata
     1441 aaacatatca ttgccggaat cgtcgtattc tttacgcgct aagaattcac gtaataaagg
     1501 cagattatca tcttcgggag actgccaata ttgccgacca aaggtataac cggcaaagta
     1561 ttgccgccag ttactataat aacgtagagc ccgtagatga atacgcgact ggatccatag
     1621 actttcttct tcactcaacc agttattaag tacgcccatt cgacttaaaa aacccatccg
     1681 gacataatcc catgctttaa ttcctccctc accacagcat tcggctgtgc tggcgacaaa
     1741 ttgcgcataa gcctgacctt gctcatttaa ttcagcaaga tagtcacgcc attcacatgg
     1801 agaataacga aaccagcggt gataaaaccc tgccaaccgc gcggcatgac cgttatcgat
     1861 cattcggtga attgtgcgca gcaggtcatc acggtcatca attccccagg aggaagaaag
     1921 gtcaaaaaag cgtgtaccta aatacatttt gggtgatgaa tgcgaaggta aacagcattc
     1981 attgcgtatt tccatcggag tggaaagtgc aaacaatatt ttttgcgact ccatgtccat
     2041 atgatttatc cttaaatatt cattgaatga atattagccg cattgcgacg ctcgtttatt
     2101 tatacttttc tgacgcgaca taatacgcat aaaataaggc ttgcgcaaaa aatgaaacac
     2161 ataaaaattc aatatatttt atctacaatc ataaggaaat gataatgatt ttcacgtcaa
     2221 gttgctgcga taatttatca atagatgaga ttatcgaacg tgctgaaaaa ggcgattgcg
     2281 aggctcaata tattgttggg ttttattata atcgcgatag cgcaattgac tctccggacg
     2341 acgaaaaggc cttttactag ctgaagctgg ccgctgagca aggtcattgt gaagcacagt
     2401 attccttagg gcaaaagtat accgaggata aaagccgtca taaagataat gagcaagcca
     2461 tcttctggct gaaaaaagct gccctacaag gccatacttt cgcttccaac gcccttggct
     2521 ggatactgga tcgtggagaa gaccccaact ataaagaagc ggttgcctgg tatcagatag
     2581 ccgcggagag cggaatgtct tatgcgcaaa ataatcttgg gtggatgtac agaaatggca
     2641 acggagccgc acaagactat acgctggcat ttttctggta caaacaagct gcattacaag
     2701 gccatagtga cgcgcaaaac aatctggccg atctttatga agacggaaaa ggcgttgctc
     2761 aaaacgagac actcgccgca ttctggtatt tgaaaagcgc acagcagggt aatcggcatg
     2821 ctcagtttca aattgcatgg gattataacg ctggcgaagg ggtggaccag gactataagc
     2881 aagcgatgta ctggtatctg aaggctgccg ctcaggggag cgtcggcgct tacgtcaaca
     2941 tcggttatat gtataaacac ggacaaggcg ttgagaaaga ttatcaggct gcctttgaat
     3001 ggtttacgaa agccgctgaa tgcaatgacg ccactgcctg gtataacctg gccattatgt
     3061 atcactacgg agaaggaaga cctgtcgatc tccgacaggc tctcgacctg tatcgtaaag
     3121 ttcagtcatc cggaaccaga gatgtcagtc aggaaattcg tgagactgaa gatttactgt
     3181 agagataaaa acaagggccg gggaatttca ttcggcccat attctcttta ttgctgtgcg
     3241 aaatcagact attacggctc aaacgggcgt ctctggaact ggtcatgacc acatttcggg
     3301 catagcgtca gcacttccgg tgtgtagatc gggagatgga agtgacattt ctcgcagacc
     3361 agatttccca gcccgaccac ttctccgctg tgataaaccc catgatgatt gaggtcctgg
     3421 aaaacttcgc gccattcaag ctgcgtttta tcggtgatgt ctgccagctc ctgccacaag
     3481 ctttctttaa tcacccgcat aaagacgctg tcagattctt ctttcaggct ctcttcatag
     3541 ctcatggcga actcttccag gtcacgtctg acagctcgcg tcagctcatc gacctcggtt
     3601 cgcgttaact cccctgtttt tattacgcgc tcgcgcgcct gttccaccag tgcgtcgata
     3661 tcacgttcgc cattgcgcag gcgttcgctc agtgacgcaa ccagttcacg gtaatattga
     3721 gcaaccttgt tcatcgtttc atctcccggg tcgttaactg actataataa tagacgttct
     3781 ttgttccttg ctttgctaat acgcccacaa actctgtaaa ttaatgtatg aataatccgg
     3841 gcatcatttc aacgtcgagt gcgcgaaagg ctgttttgac gcggacgttt gggctatgct
     3901 atgcggatct gaaaaaccac ctctaacgct acttttgtag ccgtaatgaa aacaggacca
     3961 ctggctgcca tgcaagagca ataccgcccg gaagagatag aatccaaagt acagcttcat
     4021 tgggatgaga agcgcacatt tgaagtaacc gaagacgaga gcaaagagaa gtattactgc
     4081 ctgtctatgc ttccctatcc ttctggtcga ctacacatgg gccacgtacg taactacacc
     4141 atcggtgacg tgatcgcccg ttaccagcgc atgctgggca aaaacgttct gcagccgatc
     4201 ggctgggacg cgtttggtct gcctgcggaa ggcgcggcgg tgaaaaacaa caccgctccg
     4261 gcaccgtgga cgtacgacaa catcgcgtat atgaaaaacc agctcaaaat gctgggcttt
     4321 ggttatgact ggagccgcga gctggcaacc tgtacgccgg aatactaccg ttgggaacag
     4381 aaattcttca ccgagctgta taaaaaaggc ctggtatata agaagacttc tgcggtcaac
     4441 tggtgcccga acgaccagac cgtactggcg aacgaacaag ttatcgacgg ctgctgctgg
     4501 cgctgcgata ccaaagttga acgtaaagag atcccgcagt ggtttatcaa aatcactgct
     4561 tacgctgacg agctgctcaa cgatttggat aaactggatc actggccaga caccgttaaa
     4621 accatgcagc gtaactggat cggtcgttcc gaaggcgtgg aaatcacctt caacgttaac
     4681 gactatgaca acacgctgac cgtttacact acccgcccgg acacctttat gggttgtacc
     4741 tacctggcgg tagctgcggg tcatccgctg gcgcagaaag cggcggaaaa taatcctgaa
     4801 ctggcggcct ttattgacga atgccgtaac accaaagttg ctgaagctga aatggcgacg
     4861 atggagaaaa aaggcgtcaa tactggcttt aaagctgttc acccattaac gggcgaagaa
     4921 attcccgttt gggcagcaaa cttcgtattg atggagtacg gcacgggcgc agtaatggcg
     4981 gttccggggc acgaccaacg cgactacgag tttgcctcta aatacggcct gaacatcaaa
     5041 ccggttatcc tggcggctga cggctctgag ccagatcttt ctcagcaagc cctgactgaa
     5101 aaaggcgtgc tgttcaactc tggcgagttc aacggtcttg accatgaagc ggccttcaac
     5161 gccatcgccg ataaactgac tgcgatgggc gttggcgagc gtaaagtgaa ctaccgcctg
     5221 cgtgactggg gtgtttctcg tcagcgttac tggggcgcgc cgattccgat ggtgacgctg
     5281 gaagacggta ccgtaatgcc gaccccggac gaccagctgc cggtgatcct gccggaagat
     5341 gtggtgatgg acggcattac cagcccgatt aaagcagatc cacagtgggc gaaaactacc
     5401 gttaacggta tgccagcgct gcgtgaaacc gacactttcg acacctttat ggagtcctcc
     5461 tggtactatg cgcgctacac ttgcccagag tacaaagaag gtatgctgga ttccgaagcg
     5521 gctaactact ggctgccggt ggatatctac attggtggta tcgaacacgc cattatgcac
     5581 ctgctctact tccgcttctt ccacaaactg atgcgtgatg caggcatggt gaactctgac
     5641 gaaccagcga aacagttgct gtgtcagggt atggtgctgg cagatgcctt ctactatgtt
     5701 ggcgaaaacg gcgaacgtaa ctgggtttcc ccggttgatg ctatcgttga acgtgacgag
     5761 aaaggccgta tcgtgaaagc gaaagatgcg gcaggccatg aactggttta taccggcatg
     5821 agcaaaatgt ccaagtcgaa gaacaacggt atcgacccgc aggtgatggt tgaacgttac
     5881 ggcgcggaca ccgttcgtct gtttatgatg tttgcttctc cggctgatat gactctcgaa
     5941 tggcaggaat ccggcgtgga aggggctaac cgcttcctga aacgtgtctg gaaactggtg
     6001 tacgagcata ctgcgaaagg tgatgttgcg gcactgaacg ttgatgcact gactgaagat
     6061 cagaaagcgc tgcgtcgcga tgtgcataaa actatcgcta aagtgaccga tgatatcggc
     6121 cgtcgtcaga ccttcaacac cgcaattgcg gcgattatgg agctgatgaa caaactggcg
     6181 aaagcaccga ccgatggcga gcaggatcgc gctctgatgc aggaagcact gctggccgtt
     6241 gtccgtatgc ttaacccgtt caccccgcac atctgcttca cgctgtggca ggaactgaaa
     6301 ggcgaaggcg atatcgacaa cgcgccgtgg ccggttgctg acgaaaaagc gatggtggaa
     6361 gactccacgc tggtcgtggt gcaggttaac ggtaaagtcc gtgctaaaat caccgttccg
     6421 gtggacgcaa cggaagaaca ggttcgcgaa cgtgctggcc aggaacatct ggtagcaaaa
     6481 tatcttgatg gcgttactgt acgtaaagtg atttacgtac caggtaaact cctcaatctg
     6541 gtcgttggct aagcgcggga ggaagcgtgc gatatctggc aacattgttg ttatctctgg
     6601 cggtgttaat caccgccggg tgtggctggc atctgcgtga caccacgcag gttccttcca
     6661 ctatgaaggt catgatcctg gactcaggcg atccgaacgg gccattaagc cgtgcggtgc
     6721 gtaaccagtt acgtctgaat ggtgtcgagt tgcttgataa agaaaccacg cgtaaggacg
     6781 ttccatccct gcgtttgggt gctgtatcaa tctcacagga taccgcgtcg gttttccgta
     6841 atggtcaaac tgcggaatat cagatggtga tgactgttag cgcgtcggta ttgattcctg
     6901 gacgagatat ctacccgatt agcaccaaag tcttccgttc attcttcgat aacccgcaaa
     6961 tggcgttagc gaaagataac gagcaggaaa tgatcattaa agagatgtac gaccgtgctg
     7021 ccgaacagct gattcgtaag ctgccaagca tccgtgctgc ggatattcgt tccgacgaag
     7081 aacagacgtc gactacaacg gatactccgg caacgcctgc acgcgtctcc accacgctgg
     7141 gtaactgatg attcggttgt acccggaaca actccgcgcg cagctcaatg aagggctgcg
     7201 cgcggcgtat cttttacttg gtaacgatcc tctgttattg caggaaagcc aggacgctgt
     7261 tcgtcaggta gctgcggcac aaggattcga agaacaccac actttttcca ttgatcccaa
     7321 cactgactgg aatgcgatct tttcgttatg ccaggccatg agtctgtttg ccagtcgaca
     7381 aacgctattg ctgttgttac cagaaaacgg accaaacgcg gcgatcaatg aacaacttct
     7441 cacactcacc ggacttctgc atgacgacct gctgttgatc gtccgcggta ataaattaag
     7501 caaagcgcaa gaaaatgccg cctggtttac tgctcttgcg aatcgcagcg tgcaggtgac
     7561 ctgtcagaca ccggagcagg ctcagcttcc ccgctgggtt gctgcgcgcg caaaacagct
     7621 caacttagaa ctggatgacg cggcgaatca ggtgctctgc tactgttatg aaggtaacct
     7681 gctggcgctg gctcaggcac tggagcgttt atcgctgctc tggccagacg gcaaactgac
     7741 attaccgcgc gttgaacagg cggtgaatga tgccgcgcat ttcactcctt ttcattgggt
     7801 tgatgctttg ttgatgggaa aaagtaagcg cgcattgcat attcttcagc aactgcgtct
     7861 ggaaggcagc gaaccggtta ttttgttgcg cacattacaa cgtgaactct tgttactggt
     7921 taacctgaaa cgccagtctg cccatacgcc actgcgtgcg ttgtttgata agcatcgggt
     7981 atggcagaac cgccggggca tgatgggcga ggcgttaaat cgcttaagtc agccgcagtt
     8041 acgtcaggcc gtgcaactcc tgacacgaac ggaactcacc ctcaaacaag attacagtca
     8101 gtcagtgtgg gcagagttgg aagggttatc tcttctgttg tgccataaac ccctggcgga
     8161 cgtatttatc gacggttgat atgaaatctt tacaggctct gtttggcggc acctttgatc
     8221 cggtgcacta tggtcatcta aaacccgtgg aaacgctggc gaatttgatt ggtctgacgc
     8281 gagtcacaat catccctaat aatgttcctc cgcatcgtcc ccagccggaa gcgaacagcg
     8341 tacagcgtaa acacatgctt gaactggcga ttgccgacaa gccattattt actcttgatg
     8401 aacgcgagct aaagcgcaat gccccctctt acactgcgca aacactgaaa gagtggcggc
     8461 aggaacaagg gccggacgtg ccgctggcgt ttattattgg tcaggattca ttgctgacct
     8521 ttccgacctg gtacgaatac gaaacgatac tcgacaatgc acatttgatc gtctgtcggc
     8581 gtccaggtta cccacttgaa atggcgcaac cgcaatacca gcaatggctg gaagatcatt
     8641 tgacacataa cccggaagat cttcaccttc agcctgccgg taaaatttat ctggctgaaa
     8701 cgccgtggtt taacatctcg gcgaccatca tccgcgaacg tttgcaaaac ggtgaatcat
     8761 gtgatgattt attgccggaa ccggtactga cttacattaa ccaacaaggc ttgtatcgct
     8821 gatacctgtc gttacggagg gcatgatgcg actgtggtta attcgtcatg gcgaaacaca
     8881 agcgaatgtc gatggtctgt acagcggtca tgcgcccaca cccctgaccg cacgcggtat
     8941 tgagcaagcg caaaatctgc atacactgct agatgatgtt tcctttgacc tggttttatg
     9001 cagtgaactg gaacgggcac agcataccgc gcgactggtt ctcagtgacc gccagctccc
     9061 cgttcatatc atccctgaac tcaacgaaat gttttttggc gactgggaga tgcgacatca
     9121 tcgcgacctc atgcaagaag atgccgaaaa ctatagcgcg tggtgcaatg actggcagca
     9181 tgctatcccc acgaacggtg aaggatttca ggcattttcg caacgtgtgg aacgctttat
     9241 cgcaaggctt agtgaatatc agcactatca gaatatttta atcgtcagcc atcagggcgt
     9301 acttagtctg ttaattgctc gtttaattgg catgcctgcc gagtctatgt ggcattttcg
     9361 cgttgaccag gggtgttgga gcgccattga tataaaccaa aaattcgcga cgctacgcgt
     9421 cctcaatagc cgtgccatcg gggtcgagaa tgcatgactt ttctgttttt ttacgggtaa
     9481 gccgcaacga ccattgacag ccccgggcag gctgatattc tccgcagcca gactttttcc
     9541 gccagacacg actttgtaga aattgtttta caaaaatggc gatgcaatct gcggcgcggg
     9601 gtgggatgat agcccacttt cgaaagccga ttcggcgaca attgtcccga aatcgcctct
     9661 ggttcaggta tactgacaga ccatttttat ctatttgatt cacccagggg gaaaacttgc
     9721 agggtaaagc actccaggat tttgttatcg acaaaattga tgacctcaaa ggtcaggaca
     9781 tcatcgcctt agacgttcag ggcaaatcca gcatcactga ctgcatgatc atctgtacgg
     9841 gtacgtccag ccgtcatgtt atgtccattg ctgaccacgt tgtgcaggag tctcgcgcag
     9901 cgggcctgtt accgctcgga gtagaaggtg aaaacagcgc cgactggatt gtcgtggatt
     9961 tgggcgatgt gattgtccat gtcatgcagg aagagagccg tcgcctgtat gaactggaaa
    10021 aactctggag ttaatgcgtg aagctgcaac ttgtcgccgt gggaacgaaa atgccggact
    10081 gggtacaaac cggttttacc gagtacctgc gtcgttttcc gaaagatatg cccttcgagc
    10141 tgattgaaat tccggccgga aaacgcggca agaatgcgga catcaagcgc atactcgaca
    10201 aagagggtga gcagatgttg gcggccgcag gcaaaaaccg cattgtcacc ctcgatattc
    10261 caggcaagcc ctgggatacg ccgcagttag ccgctgagct ggaacgctgg aagctggatg
    10321 gtcgcgacgt cagtctactg attggcgggc ctgaagggtt gtcgcctgcc tgtaaagcgg
    10381 cggcggagca gagctggtcg ctgtcggcgc ttaccctccc ccatccgctg gttcgcgtgc
    10441 tggtcgcaga gagtctgtac cgggcgtgga gcatcaccac caaccatcct tatcaccgtg
    10501 agtgataagg gagctttgag tagaaaacgc agcggatgaa actacagaac tcttttcgcg
    10561 actatacggc agagtccgcg ctgtttgtgc gccgggcgct ggtcgccttt ttggggattt
    10621 tgctgctgac cggcgtgctt atcgccaacc tgtataatct gcaaattgtt cgctttaccg
    10681 actaccagac ccgctctaat gaaaaccgca ttaagctggt gcctatcgcg cccagccgcg
    10741 gcattatcta cgaccgtaac ggtatccctc tggccctcaa ccgcactatc taccagatag
    10801 aaatgatgcc ggagaaagtc gataacgtgc agcaaacgct ggacgctttg cgcagcgtgg
    10861 ttgatctgac cgatgacgat attgctgcat tccgaaaaga gcgcgcacgt tcacaccgtt
    10921 tcacctccat tccggtgaaa actaacctga ccgaagtaca agtagctcgc tttgccgtca
    10981 atcagtaccg ttttccgggt gttgaagtta aaggctataa acgtcgttac tatccttacg
    11041 gttcggcgtt gacccacgtc atcggctatg tgtcgaaaat caacgataaa gacgtcgaac
    11101 gcctgaataa tgacggcaaa ctggccaact atgcggcaac gcatgatatc ggtaagctgg
    11161 gcattgagcg ttactatgaa gacgtgctgc acggtcagac cggttatgaa gaggttgaag
    11221 ttaacaaccg tgggcgtgtt atccgccagt taaaagaagt cccaccgcaa gccggacacg
    11281 atatttacct gacgctggat ctcaaactcc agcaatatat tgaaacgctg ctggcgggta
    11341 gccgcgcagc tgtggtagtc accgatccgc gtacaggtgg ggtgctggcg ctggtttcca
    11401 cgcctagtta tgacccaaac ttgtttgttg acggtatctc cagcaaagat tattccgcct
    11461 tgttgaacga cccgaataca ccgctggtga accgcgccac acagggggtt tatcctcccg
    11521 cgtctacagt taaaccctat gtggcggttt cggcattgag cgccggggtg atcacgcgca
    11581 atacgacgct gtttgaccct ggctggtggc aactgccagg ttcggaaaaa cgttatcgtg
    11641 actggaaaaa atggggccac gggcgtctga atgtcacaag atcgctggaa gaatctgcgg
    11701 ataccttctt ctatcaggtg gcctacgata tggggatcga tcgcctctcc gaatggatgg
    11761 gtaaattcgg ttatggtcat tataccggta tcgacctggc ggaagaacgt tccggcaata
    11821 tgcctacccg cgaatggaaa cagaaacgct ttaaaaaacc gtggtatcag ggtgacacca
    11881 ttccggttgg tatcggtcag ggttactgga cagcgacccc aatccagatg agtaaggcac
    11941 tgatgatcct gattaatgac ggtatcgtga aggttccgca tttgctgatg agcaccgccg
    12001 aagacggcaa acaggtgcca tgggtacaac cgcatgaacc gcccgttggc gatattcatt
    12061 ccggttactg ggagctggcg aaagacggta tgtacggtgt tgctaaccgc cctaacggta
    12121 cggcgcataa atactttgcc agcgcaccgt acaaaattgc ggcgaaatct ggtaccgctc
    12181 aggtcttcgg tctgaaagcg aacgaaacct ataatgcgca caaaattgcc gagcgtttac
    12241 gtgaccacaa actgatgacc gcctttgcgc catacaacaa tccgcaagtg gctgtcgcca
    12301 tgattctgga gaacggtggc gcgggtccgg cggttggtac gctgatgcgc cagatcctcg
    12361 accacattat gctgggtgat aacaacaccg atctgcctgc ggaaaatcct gcggttgccg
    12421 cagcggagga ccattaatca tgacggataa tccgaataaa aaaacattct gggataaagt
    12481 ccatctcgat cccacaatgc tgctgatttt actggcattg ctggtttaca gcgccctggt
    12541 tatctggagc gccagcggtc aggatattgg catgatggag cgtaaaatcg gccaaatcgc
    12601 gatgggtctg gtcatcatgg tggtgatggc gcaaattcct ccacgcgttt atgaaggctg
    12661 ggccccctat ctctatatca tctgtattat tttgctggtg gcggtagatg ctttcggtgc
    12721 catctctaaa ggcgctcaac gctggctgga cctcggtatt gttcgttttc agccgtcgga
    12781 aattgccaaa atagccgtac cactgatggt tgcgcgcttt atcaaccgcg acgtttgccc
    12841 gccatcgttg aagaacactg gcatcgcgct ggtgctgata tttatgccca cgctgctggt
    12901 ggctgcacag cctgacctgg gaacatcaat cctcgttgcg ctttccggtc tgtttgtact
    12961 gttcctctct ggccttagct ggcgtctgat tggcgtcgca gtagtgctgg tagcggcgtt
    13021 cattccgatt ctgtggttct tcctgatgca tgattaccag cgccagcgcg taatgatgct
    13081 cctggacccg gaatcagacc cactcggcgc gggctatcac attattcagt ctaaaattgc
    13141 tattggctcc ggcggattac gtggcaaagg ctggctgcac ggcactcagt cacagcttga
    13201 atttctcccc gaacgccata ctgactttat cttcgcggta ctggcggaag agctgggatt
    13261 agtgggcatt ttgattctgc tcgctctcta cattctgctg atcatgcgcg ggctgtggat
    13321 agccgccaga gcgcaaacca cctttggtcg cgtcatggct ggcggcttaa tgctgatatt
    13381 attcgtttat gtcttcgtaa atattggtat ggtaagcggt attctgccgg ttgtaggggt
    13441 tccgctccca ctggtcagtt atggaggatc ggcgctaatt gtgctgatgg ctgggttcgg
    13501 gattgtaatg tcaatccaca cccacaggaa aatgttgtcg aaaagcgtgt aagaggtgcg
    13561 caatgcgtaa gcagtggctc gggatctgca tcgcggcagg aatgctcgcg gcatgtacaa
    13621 gcgatgatgg tcagcaacag acagtaagtg taccgcagcc tgcggtatgt aacggcccca
    13681 tagttgaaat tagcggggcg gacccgcgtt tcgaaccact gaacgcgacg gcaaatcagg
    13741 attaccagcg cgacggtaaa agctacaaaa tcgtgcagga tccgtctcga tttatccagg
    13801 cgggactggc ggcaatctat gatgccgaac caggcagtaa cctgacggcc tctggcgaag
    13861 ctttcgatcc gacacagctg acggcggccc atccaacgct tccgatcccc agctacgcca
    13921 gaatcactaa cctggctaac gggcgaatga tcgtggtgcg cattaatgat cgcggtcctt
    13981 acggcaacga ccgcgttatt tcgctttctc gcgcagcagc tgaccgtctt aacacgtcaa
    14041 acaacaccaa agttcgtatc gatccgatta ttgtcgccca ggatggttcg ctttctggtc
    14101 ctggtatggc ttgtaccaca gtcgccaaac agacttacgc cctgcctgca cctcccgatt
    14161 taagcggtgg cgcgggaaca agttcagtgt ctggcccgca gggtgacatt cttccggtca
    14221 gtaattcgac gctaaaaagc gaagatccga ccggcgcgcc ggtaaccagc agcggtttcc
    14281 tcggcgcacc aacgacctta gcgccaggtg tactggaagg cagcgaaccg acgcctgctc
    14341 cacagcccgt tgttacagct ccgtcgacaa cgcctgcaac ctcgcctgca atggtgacac
    14401 cgcaagccgc ctcgcaaagc gccagcggca actttatggt gcaggtcggg gccgtaagcg
    14461 atcaggctcg tgcgcaacag taccaacagc aactgggaca gaagttcggc gtccccggtc
    14521 gcgtaactca aaatggcgcg gtctggcgga tccagcttgg cccattcgcc aacaaagccg
    14581 aagccagtac cttgcagcaa cgtttgcaaa ccgaagccca attacagtca tttattacca
    14641 ccgcgcagta gcgtaaagca ggcatctgat gtgtcaattt ctgtaaatga cgttaacaaa
    14701 gtcacgcgga aagtcagatg cctgccggta gtgcatttgc tatagtaggg cacttttttt
    14761 aattccatca cggatgtcgt agttcagacc atgaatacca ttttttccgc tcgtatcatg
    14821 aagcgcctgg cgctcaccac ggctctttgc acagccttta tctctgctgc acatgccgat
    14881 gacctgaata tcaaaactat gatcccgggt gtaccgcaga tcgatgcgga gtcctacatc
    14941 ctgattgact ataactccgg caaagtgctc gccgaacaga acgcagatgt ccgccgcgat
    15001 cctgccagcc tgaccaaaat gatgaccagt tacgttatcg gccaggcaat gaaagccggt
    15061 aaatttaaag aaactgattt agtcactatt ggcaacgacg catgggccac cggtaacccg
    15121 gtgtttaaag gttcttcgct gatgttcctc aaaccgggca tgcaggttcc ggtttctcag
    15181 ctgatccgcg gtataaacct gcaatcgggt aacgatgctt gtgtcgccat ggccgatttt
    15241 gccgctggta gccaggacgc ttttgttggc ttgatgaaca gctacgttaa cgccctgggc
    15301 ctgaaaaata cccacttcca gacggtacat ggtctggatg ctgatggtca gtacagctcc
    15361 gcgcgcgata tggcgctgat cggccaggcg ttgatccgtg acgtaccgaa tgaatactcg
    15421 atctataaag aaaaagaatt tacgtttaac ggtattcgcc agctgaaccg taacggcctg
    15481 ttatgggata acagcctgaa tgtcgacggc atcaaaaccg gacacactga caaagcaggt
    15541 tacaaccttg ttgcttctgc gactgaaggc cagatgcgct tgatttctgc ggtgatgggc
    15601 ggacgtactt ttaaaggccg tgaagccgaa agtaaaaaac tgctaacctg gggcttccgt
    15661 ttcttcgaaa ccgttaaccc actgaaagta ggtaaagagt tcgcctctga accggtttgg
    15721 tttggtgatt ctgatcgcgc ttcgttaggg gttgataaag acgtgtacct gaccattccg
    15781 cgtggtcgca tgaaagatct gaaagccagc tatgtgctga acagcagtga attgcatgcg
    15841 ccgctgcaaa agaatcaggt cgtcggaact atcaacttcc agcttgatgg caaaacgatc
    15901 gaacaacgcc cgctggttgt gttgcaagaa atcccggaag gtaacttctt cggcaaaatc
    15961 attgattaca ttaaattaat gttccatcac tggtttggtt aaaaattgaa cacttgaaag
    16021 tgtaatttcc gtccccatat actaagcatc agtaaaaaac tcccgccttc tggcgggagt
    16081 tgctatttaa ttacgttacg ccggagctga catgaaaacc aaacttaacg aactgcttga
    16141 attccctact ccttttactt acaaagttat ggggcaggcg ttacctgagc tggttgatca
    16201 ggtggttgaa gtggtacagc gccatgcgcc aggtgactac accccaacgg taaaaccaag
    16261 cagcaaaggc aactaccact cggtatctat cactatcaac gccactcata tcgagcaggt
    16321 tgaaacactg tatgaagaac tgggcaaaat cgatattgtc cgcatggttc tgtaactcgc
    16381 ttctccgtta cccggcccct ggtcgggtaa ctcccctctc ctcgctgtga tatacttttc
    16441 tcccactttt actcattctc cacggagatg ccgttttgta tcaggataaa attcttgtcc
    16501 gccagctcgg tcttcagcct tacgagccaa tctcccaggc tatgcatgaa ttcaccgata
    16561 cccgcgatga tagtaccctt gatgaaatct ggctggtcga gcactatccg gtattcaccc
    16621 aagggcaggc aggaaaggcg gagcacattt taatgcccgg tgatattccg gtgattcaga
    16681 gcgatcgcgg tgggcaggtg acttatcacg ggccggggca acaggtgatg tatgtattgc
    16741 ttaacctgaa acgccgtaaa ctcggtgtgc gtgaactggt gaccttgctt gagcaaacag
    16801 tggtgaatac cctggctgaa ctgggtatag aagcgcatcc tcgggctgac gcgccaggtg
    16861 tctatgttgg ggaaaagaaa atttgctcac tgggtttacg tattcgacgc ggttgttcat
    16921 tccacggtct ggcattaaac gtcaatatgg atctttcacc atttttacgt attaatcctt
    16981 gtgggtatgc cggaatggaa atggctaaaa tatcacaatg gaaacccgaa gcgacgacta
    17041 ataatattgc accacgttta ctggaaaata ttttagcgct actaaacaat cctgacttcg
    17101 aatatattac cgcttaattt catacctcaa tggcccaatt tacactgggt cattactcat
    17161 aacacctgct aatgctttac atctccttcg cgtttgtata ttttctttat cacttgcaga
    17221 aatgcgatgc tacgccttgc tccgtaactc accccattga ttaacttatt gaaatagttg
    17281 attatgatga gcaaaagcgt tccgttccaa ttagacataa gattattgta atttttttaa
    17341 atattatttt tccatgaata aaattggagt aagtgcgtgg atagtaataa tcaaattgaa
    17401 tcctgtttaa gcagaaaatc gtcagaaggt aaaccacaaa tatttacaac cttacgaaat
    17461 attgatctta atcttctgac tatttttgaa gctgtatatg tacataaagg gatcgttaat
    17521 gcagcgaaag tgcttaatct gaccccctcg gcaatcagtc agtctattca gaaactgcgc
    17581 gttatattcc ctgacccatt gtttattcgc aaaggccagg gtgtcactcc taccgcattt
    17641 gcgatgcatc tacatgagta tatcagtcag ggccttgagt ccattcttgg cgcgctggat
    17701 atcgaaggaa gctatgataa gcaacgaacg ataactattg cgaccacccc ctcggtcgga
    17761 gctctggtcc ttcctgtcat ttatcgggcg attaaaactc actatccgca gcttttactg
    17821 cgcaacccgc ccatcagcga cgcggaaaac caactcagtc agtttcaaac cgatctcatc
    17881 atagataaca tgttttgcac caatcgtacg gtgcaacatc atgttctgtt caccgacaat
    17941 atggtgttaa tttgccgcga gggaaatcca ctactctctt tagaagatga cagagagact
    18001 atcgacaacg ctgcacatgt actcctgtta ccggaaggac aaaatttcag cggtctgcgg
    18061 cagagaattc aagagatgtt tccggaccgg cagatcagct tcaccagcta caacattttg
    18121 acaatcgctg cactggttgc caacagtgac atgttagcga ttattcccag ccgtttttat
    18181 aacctgttta gccgttgctg gccgctggaa aaactgcctt ttccgtcctt aaatgaggag
    18241 caaatagatt tctctatcca ctacaacaaa ttcagcctgc gtgatcccat cctgcacgga
    18301 gtaatagatg ttatccgtaa tgcattttga aaaagtattg ttacagccag atcacagcgc
    18361 agaacgataa aatggcacaa tccaacaaca attttacatt ttcgcgaccg ctttggctgc
    18421 tttcaggtcc gtttcaatga tatactgcca gtcgttaatt caaaaatagt tgataattac
    18481 aacaatctat tgaattgaaa cgctttcctt cgtaattcgc aactggaaca cgcacgctat
    18541 gagtaaaccc attgtgatgg aacgcggtgt taaataccgc gatgccgata agatggccct
    18601 tatcccggtt aaaaacgtgg caacagagcg cgaagccctg ctgcgcaagc cggaatggat
    18661 gaaaatcaag cttccagcgg actctacacg tatccagggc atcaaagccg caatgcgcaa
    18721 aaatggcctg cattctgtct gcgaggaagc ctcctgccct aacctggcgg aatgcttcaa
    18781 ccacggcaca gcaacgttta tgatcctggg cgctatttgt acccgccgtt gtccgttctg
    18841 tgacgttgcc cacggtcgcc cggtagctcc tgatgccaat gagccagtga aactggcgca
    18901 gaccattgcc gatatggcgc tgcgttatgt ggttatcacc tccgttgacc gtgatgacct
    18961 gcgcgatggc ggtgcccaac actttgcgga ttgtattact gccattcggg aaaaaagccc
    19021 gcaaatcaaa attgaaactc tggtgccgga tttccgcggt cgtatggatc gtgctctgga
    19081 tattctgact gcaacgcccc cagatgtgtt caaccataac ctggaaaacg taccgcgtat
    19141 ttaccggcag gtacggcctg gcgcagatta caactggtcg ctgaagctgc tggaacgctt
    19201 taaagaagcg catccggaaa tcccgaccaa gtctggtctg atggtgggtc tgggtgaaac
    19261 caatgaagaa attattgagg taatgcgcga cctgcgccgt catggtgtga cgatgttaac
    19321 gctggggcaa tatttgcagc caagccgcca tcacctgccg gttcaacgtt acgttagccc
    19381 ggatgagttc gacgaaatga aagccgaagc gctggcgatg ggctttaccc atgctgcatg
    19441 cggtccgttt gtccgctctt cttaccacgc cgatttgcag gcgaaaggga tggaagttaa
    19501 gtaatcctgt aataattact tacatctgtc tgataaaaaa cccgcttttt gaagcgggtt
    19561 ttttgtatcg aacaagatat tgagggagcg tcctgctcgc cacgtcactc tttatgagag
    19621 agcttttcag cctgaagatc aacgtctgcg ccttttttcg ccgcagcatc gtcatcattc
    19681 atcgccttct tgaacccttt aatggccgct ccaaggtctc cgcccagcgt acgtaacttc
    19741 ttagtcccaa acagcagaac gaccagcgcc gcaactacca gcagtttggt aatactaatc
    19801 tcacccatag ataccttctt gacatataac cggcaacaga acgcgcctta aaaaccaaag
    19861 aatattaacg gtcattcgac gcgcgcacac aatagcaatc tgtaacaagg tgaatcaagc
    19921 gccgagtaaa aaaacatcat aataattgcg gcggcgcaaa gcgacggttg tttaagacgg
    19981 gcagttgcgc gcgcacctga cgcacacgtt cgggcgtcac ttccgccata atgagtgcag
    20041 gcatttctga cgctgccgca atggtgacgc caaagggatc tataatccgg ctttgcccga
    20101 tatttttgtt cccgcactcc cccgccgcca ccatataaca ggtggtatcc agcgcacgag
    20161 cggcaagcaa cgttgaccag tggtgctctt tgagcggccc gcgaacccag gcggcaggaa
    20221 gtaccaggat ttcagctccc tgtaatgcct gtgccagcgc cagctctgga aagcgtaagt
    20281 cataacaggt catcagaccg accttcatcc cctccacctc gagtaacgga gcaatttcat
    20341 taccagcatc aacacggcgt gattcctgaa tggcaaatgc atcatagaga tgcaatttgg
    20401 cataacgggc gacgatgttt cctgcctgta gcgccaccag catattccat gcgcgccccg
    20461 gcgttgaagg aacatgaatc gtcagaattg tcgtcatcat gttacgttta ctttctcgcc
    20521 gtaaacgtcc gaggaattcg ccttccagca gctgtgctga tttaaccgat agatctgcat
    20581 catgatcatc gcgcgccagc aatgcttccg gcaggacaaa cagcgatacg tcgttttccg
    20641 ccgcctgcgc catcaacgag gcacaaatct cagcgttctt ttcccacaca gatgtaacag
    20701 caaactgtcc tgcagcaacc agcattgcca cctcctgtca gagtcgtaaa attcgccaac
    20761 gacgattatt cggcgttaca cttatcactc atacaaatca aatagcagga ttttgcagtg
    20821 ttacaacttc ttttagcagt ttttattggc ggtggtacgg gaagcgtggc gagatggctg
    20881 ttaagtatgc gatttaaccc gctgcatcag gcgattccgt tggggacgct ggcagcaaac
    20941 ctgattgggg cattcatcat aggaatggga ttcgcctggt tcagcaggat gacgaacatt
    21001 gatccagtgt ggaaagtatt aatcaccacc ggattttgtg gcggtctaac aaccttctca
    21061 acattttcgg cagaagtggt gtttttgtta caagagggcc gctttggctg ggcattactg
    21121 aacgttttcg tcaaccttct ggggtctttt gccatgaccg cactggcatt ctggctgttt
    21181 tcggcctcaa ccgcacacta aagaccaaaa aaaacccgct gattaagcgg gttttgaatt
    21241 cttgctgacg tatcttacag agcgattacg tttgcagcag aagggccttt ggcaccgtta
    21301 gtgatttcga actctacgcg ctgaccttca gcaagagttt taaaaccatt agtctggatt
    21361 gcagagaagt gtacgaacac gtctttgctg ccgtcttccg gagtaatgaa accgaatcct
    21421 ttggactcat taaaccactt aacgttacct ttaatcttag acatcaaaat tacctttaca
    21481 tgaaaaatag acacaaatgc tgtgtcggtt accagtacac caattgtggt acgctttgtc
    21541 cagtgcaact ttactaaaaa gtgataaaaa tcgcgttatt tttgatgtta cttattttag
    21601 ttattgattt taaagaagtt actaaaactt catttgtctc aaaactgaaa gcgcatccag
    21661 gcaaagtaca cattgccatt gttgtaggta cccggaatgt aggtcatctg aaaagtaact
    21721 gggccataac ccacggaggc caatggcagt agaaccggga gagggatgta attccagtta
    21781 tcgcgtgccg ttacgccagc ggtgaatccc agacctaaat gaaaattttc atccgccagc
    21841 ggtcgccagg tactttccca tccgtatccg gcaatcggtt cccatttgtt ccacgagtcc
    21901 ttaaatgcca tggcatacag gccatgccag tttccttttt catcccaacg cgacaggcca
    21961 aaaccgccac cccacggtcg ctcgttatag cgatcggttt tttctttgtc gtaagcgaaa
    22021 cgtgcatgcc aggtgatggc aggaatatat aaatcataat gttcaggctg ttgccaggtt
    22081 tgtacaatat tttctctaaa cgttgtcatc cactcatctg cgttagcaaa aactttacca
    22141 acgctgatta actgaataaa aacaaaggaa aagatagcga catatttact cacgttcatt
    22201 tgtgaccata aaacatttat caaaaatcta ctactagcat agcaaagcta caattaacat
    22261 a
//
LOCUS       DBFIFM010000066        21358 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.066, whole genome shotgun sequence.
ACCESSION   DBFIFM010000066 DBFIFM010000000
VERSION     DBFIFM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.066"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..359
                     /locus_tag="N7C74_002147"
     CDS             <1..359
                     /locus_tag="N7C74_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2432013.1"
                     /translation="LFYLNNNYREKITLEQLSKKFRASVSYICHEFTKEYRISPINYV
                     IQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFA
                     EQEILSEFPQPVSLAG"
     gene            complement(402..1553)
                     /locus_tag="N7C74_002148"
     CDS             complement(402..1553)
                     /locus_tag="N7C74_002148"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HGU2432014.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1567..3162)
                     /locus_tag="N7C74_002149"
     CDS             complement(1567..3162)
                     /locus_tag="N7C74_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HGU2432015.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(3307..4065)
                     /gene="aroD"
                     /locus_tag="N7C74_002150"
     CDS             complement(3307..4065)
                     /gene="aroD"
                     /locus_tag="N7C74_002150"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HGU2432016.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLALLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4096..4962)
                     /gene="ydiB"
                     /locus_tag="N7C74_002151"
     CDS             complement(4096..4962)
                     /gene="ydiB"
                     /locus_tag="N7C74_002151"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HGU2432017.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDKF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENKSLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4974..6239)
                     /locus_tag="N7C74_002152"
     CDS             complement(4974..6239)
                     /locus_tag="N7C74_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2432018.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(6466..7680)
                     /locus_tag="N7C74_002153"
     CDS             complement(6466..7680)
                     /locus_tag="N7C74_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2432019.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQSNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFSFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(7780..8136)
                     /locus_tag="N7C74_002154"
     CDS             complement(7780..8136)
                     /locus_tag="N7C74_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HGU2432020.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGRCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8272..8379)
                     /gene="rprA"
                     /locus_tag="N7C74_002155"
     ncRNA           complement(8272..8379)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="N7C74_002155"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8565..9677)
                     /gene="ydiK"
                     /locus_tag="N7C74_002156"
     CDS             complement(8565..9677)
                     /gene="ydiK"
                     /locus_tag="N7C74_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HGU2432021.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIQKPNK"
     gene            10066..13122
                     /locus_tag="N7C74_002157"
     CDS             10066..13122
                     /locus_tag="N7C74_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HGU2432022.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGLRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQMCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            13119..13529
                     /gene="menI"
                     /locus_tag="N7C74_002158"
     CDS             13119..13529
                     /gene="menI"
                     /locus_tag="N7C74_002158"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HGU2432023.1"
                     /translation="MIWKRKIPLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEISDEKGRLCCSSRLTTAIL"
     gene            13629..13817
                     /locus_tag="N7C74_002159"
     CDS             13629..13817
                     /locus_tag="N7C74_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HGU2432024.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14366..14734
                     /gene="sufA"
                     /locus_tag="N7C74_002160"
     CDS             14366..14734
                     /gene="sufA"
                     /locus_tag="N7C74_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HGU2432025.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14743..16230
                     /gene="sufB"
                     /locus_tag="N7C74_002161"
     CDS             14743..16230
                     /gene="sufB"
                     /locus_tag="N7C74_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HGU2432026.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVVSDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16240..16986
                     /gene="sufC"
                     /locus_tag="N7C74_002162"
     CDS             16240..16986
                     /gene="sufC"
                     /locus_tag="N7C74_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HGU2432027.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16961..18232
                     /gene="sufD"
                     /locus_tag="N7C74_002163"
     CDS             16961..18232
                     /gene="sufD"
                     /locus_tag="N7C74_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HGU2432028.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLELAEGAEATVIEHFVSLNDARHFTGARFSINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENCTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLTRIGQRLPGGAR"
     gene            18229..19449
                     /gene="sufS"
                     /locus_tag="N7C74_002164"
     CDS             18229..19449
                     /gene="sufS"
                     /locus_tag="N7C74_002164"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HGU2432029.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSAQGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19462..19878
                     /gene="sufE"
                     /locus_tag="N7C74_002165"
     CDS             19462..19878
                     /gene="sufE"
                     /locus_tag="N7C74_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HGU2432030.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDVVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            20027..21031
                     /gene="ldtE"
                     /locus_tag="N7C74_002166"
     CDS             20027..21031
                     /gene="ldtE"
                     /locus_tag="N7C74_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HGU2432031.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPTASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(21094..21330)
                     /gene="lpp"
                     /locus_tag="N7C74_002167"
     CDS             complement(21094..21330)
                     /gene="lpp"
                     /locus_tag="N7C74_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HGU2432032.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         5559 a         5222 c         5387 g         5190 t
ORIGIN      
        1 ttcttttcta tctgaataat aattatcgcg aaaaaatcac tttagaacag ttatcgaaaa
       61 aatttcgtgc cagcgtcagt tatatttgcc atgaatttac caaagagtat cgtatttccc
      121 ctattaacta tgttattcaa cggcgtatga cggaagcaaa atggtcactc actaatactg
      181 aattatcaca ggcagagatc tcctggcgtg tgggttatga aaatgtcgat cactttgcca
      241 aactgttttt gcgccatgtc ggctgttcgc ccagcgatta ccgcaggcaa tttaaaaact
      301 gttttgcgga acaagaaatc ctatctgaat ttcctcaacc ggtaagtctt gccggataaa
      361 tctggcggcg cagtttgtta agaagctgcg ccgcctgcag attatttgtt ctgatagtct
      421 ttcaggatct gccgacctgc tacgtaaatc ataatttcgt ctgtaccgcc gccaatacgt
      481 tcacaacgga catcacgcca gaagcgggag acgcgcgcct catcggtata gcccagaccg
      541 cccatgattt gaatcgcatc atcaatgact tccattgcgg tacgtgcaca gtacagtttt
      601 gccagcgccg cgctggtgcg cagtgattga tgctgatcgg cttgccatgc cactttcagc
      661 accatgttgc gcatgttgtc aatcttaatc gccatcagcg ccagtttttc ctggatcatc
      721 tggttatgac caatgggttt accaaaagcg atacgctggt tggcatagcg agcggcatct
      781 tcaaaggcac actcggcaaa gccggtgctg cgcgcggcgt tgatcaggcg ctccatctca
      841 aagttgtaca tcacattgag gaaacccatt ccttcttcgc ccaccatgtc gctctcttca
      901 acttccacgt tgtcgagata gacttcgcag gtgctgagca tatgccagcc gattttatgc
      961 agcgggttaa tcttaatgcc gggcttactg gagtcgaccc accacagggt gaaggctttt
     1021 ttgggatctt tcggttgcgg atcgcgcgcc agcaccagca tatacgggta ctctttcgca
     1081 ccggtaataa aggttttctg tccgttgatg taaaccttgc cgtttttacg cgtgtaagtg
     1141 gtagtggcac tgttgttatc tgagccagca cctggttccg tcaacgccag ggcataggcg
     1201 ggatcacccg tttccagggt gctttctgcc gttttacgta gctgctctgc agaaccgaaa
     1261 cggcgcatac tgtgaataca ttgaccgttg gtaattaaaa atgccggagc accgcatttt
     1321 gacacttcca tcagcgccag catttgggtg acgtaatcag cagggatacc accaaattct
     1381 tccggcacgc caagcatgga aataccgtta tccgccagtg cccgcataaa ctcacgcgga
     1441 tatgtcccgt tttgatcgca ggtgcggaaa tactcttccg gaaagttagt cgtaatcagt
     1501 tcgcgaatac tggccagcag cagttcttgt tcttcagtta aagaaaaatc catcgtgata
     1561 ctcctgttaa tgagccgctt caggcaggac aaaacccatc gccgcatcga taaataatct
     1621 ttcgtccatc agtttgagtt ctggcgaaat cactggggtg aaatccattt tgtcgagaat
     1681 atctttttgt aaatcgacgc caggggcgat ttcgattaaa tgcagtccgt cttctttcag
     1741 cgtgaatacg gcgcgctcag tgatataacg aacatccagc cctcgctcga gagcgatttt
     1801 tccgctaaaa gtaatttccg gtagttcccg aataaatttc ttcacccgtc cttcctggac
     1861 gatattcaat ttgccatcgg caatttctgt ttttaaactg cccgcagtta acgtgccgca
     1921 gaaaataatt ttcttcgagg tggcactgat atcaataaat ccaccggtac ccatgatttt
     1981 gccattgaat ttatgcacgc cgacgttacc gtgctggtcg acttcagcaa aactcaaata
     2041 acaaacatcc agaccaccac cgtgataaaa atcaaactgg gacgtcatat ccagaatggc
     2101 acgggtattc acgttcgcgc caaaggcgat accctgtgaa gtaattccac caatcggacc
     2161 tgtttctacc gtcagaataa agtcatcagc acaaccttct tcccgggcga ccaggccaat
     2221 gccgtcagca ataccgacgc cgacattccc caccgcgcct ttacgcattt cgaataatgc
     2281 acgccgtgca actaatttac gttggtttag gggcaggcta agtttggtgc tgtcatcaag
     2341 ggtgaagtca ccagaaataa agcggttaac cggcgcaccg ccatatagtt ggctttgatc
     2401 cggatcgacc accacaatat ccaccagata acccggaata cggacagatt taggatgcag
     2461 tgtggctttc ttaaccattt tctgcacctg catcatcaca ataccgccgt tattgtgcac
     2521 cgcctgggca ataaccaatg cgtcgagata catcacctca tcttcaaaag tggcgtagcc
     2581 ttcactgtcg caggtggtag cgcgaatgaa agcaatatct ggcgcaatcg ctttgtaata
     2641 gagatattct ttgttatcaa actcgaccag tttaatcagg tcttctttag tgacttcatt
     2701 cagtttgccg ccttgctggc gtggatcgac aaatgtcccg atgccaatat cactaataat
     2761 accaggctgg tgggctgcgg cggcgcgtaa ggtttgtgta agtacacctt gtggatagtt
     2821 ataagcaata attttatttt gttctgcgag atcagaaata cgcggcgatt gtccccagtg
     2881 accgcataat gcccatttca ccagaccttc ttgcgccagc ggactaatac cgcggtcggc
     2941 gcgatcgcca agccctgttg gactaataat cgataaatta cgtggcgttt gagtctgttt
     3001 atatttatca gcaagagcag taattaacgt ggtggcttcc agaataccgc cgccagcgcc
     3061 taacacacaa agtgttgctt cgtcgggaat ataattcacc gcttcctgtg ccgacaggac
     3121 cggcactcgt ccattaatac gaggtggttt tacaggtttc attgttaatt cctgattcag
     3181 gtgtgatttt ccctacgcca gatggataat tagcgcacag agactcacga aatattttta
     3241 ttaaatacgg ttgaatatat ttttttagtt cggcggggga ggatattccc gccgaaatat
     3301 tattgcttat gcctgatgta aaatagttaa tactgtgcgc aaatcattta ccgagatttg
     3361 ccctggcgca gaggcttttt ttaccgcacc aaaagttgcc gccgagccaa atacttcacc
     3421 agccagacga gaaattacgc cagtttttgc cattgacatc gtgatgattg gacgatcggc
     3481 atactgctcc tgcatctcca gggtcgcggc aagcaacgcc agcacatcgc tggtactttg
     3541 cggcatcagc gcaatcttag gaatatcggc gtcgaaggac tgcattttgc gcagacgggc
     3601 aatgatttct tcggcttccg gcgttttatg gaagtcatgg ttggacatga caactttcac
     3661 atcatgcgcg tgggcgtagg cgacggtttc tttaacctga tcatcgccgg taaataactc
     3721 cagatcgatc atatcaacca ggccgctgtc gatggctgca cgattgagag caatataagc
     3781 ctcggtggaa atcgcctgct cgccgccttc tttggcactg cggaaggtaa acagcagcgg
     3841 tttttctggc atggtttcac ggagaatttt tgccgccgcc atgacagact ccacattgga
     3901 gaggtcggca aagtggtcca cacgccattc cagaatatca aagtccgctt cacgataggc
     3961 gagagcttcg gatttcactc gggcgatatc tttcgccatc agcgagacga tgattttagg
     4021 tgcgcccgta ccaatgacga gatcttttac agttacggtt ttcatttttt accctttctg
     4081 cacgcggtca gcctgtcagg caccgaaccc catgacctgt ttaacatatt ccagagggaa
     4141 atctttgcca gtccataacg tgaactgttc agccccttgc cacaacaaca tgccgtatcc
     4201 atcaatcgtt ttgcaaccag cttgttgcgc ctgctgcaat aacttcgtca tatgcgggtt
     4261 atacacgcat tcagtgacca gcagtcccgg atgtaacaga ctgatatcat taaccaatga
     4321 tttattctca aggggtttca tacccacttt tgtgccattg gttaaaatgt cggcggaagc
     4381 cagggcttca gcaaaggctt gctgatcggc gagatcggta accgtgacga cacaatcggt
     4441 gttttcgtta acccgctgcg cgaaggcgag ggctttatcg aagaacttat cccgacggtt
     4501 aaagagttta atttctttta aaccttcaat tgccccctgc gcgccaatcg ccgttgaggc
     4561 acctccggcc cctaacagca ccattgtttt gcctttgata tcaaaaccgc tctctttaat
     4621 ggcgcgaata tggcccgtgc cgtcggtgtt atagccgcgc aggtagccat catcattaac
     4681 gatggtgttg atggcaccca ccagtttggc tgctggcgtt aattcatcaa catattcaca
     4741 cgccagttgt ttgttcggca ttgatacacc agttccgcgc atttttagag cttttaatcc
     4801 ttcaattgct ccgggaaagc tatcgttatc cacttcgaag gccatatagg taaatggcaa
     4861 tcccgctttt tctaaggctt tattctgcat ttcaggcgat aaactgtggc ggataggata
     4921 agccatcaac ccaatcaatt cgtattttgc ggtaacatcc ataattaact cctttaacct
     4981 ctatgcttaa ttgtgtttaa ccgggtgcta aatttgcgct cgccaaaccg cacatcattc
     5041 tcaggaataa taaaaacgcg gtaatagcgg ataaaaacaa taattgcggt aatcagggcc
     5101 agcagcgcga aagtaaaatc gagaacaatg atatattgca ggccgatgtt cgacagataa
     5161 ccggtaatca gcggaataac aaagttagcc agtccaccca tcatcatata aatactggtg
     5221 actttggctt tgctttttgg gaaaaactct gacatgaccg aaacaccgag ctgtaaaatg
     5281 ccgccagctg ctgagaaacc gataacaaag gcgccggcat tacacaccag tggagaaggg
     5341 tacaggtaga taatggctgc tgttattgtt gccagtgcag agttaaatac attagcccag
     5401 atgggccgga ccattttttt cagtagtgcg gcaaaaataa agacacagac cagcgagccc
     5461 atactgtaat aagagatggt ttttaatgcc tcagcttctg acataccagc aaaagccatc
     5521 gcatatttgg gcatccacac cacaatcaca taaaaggtcg agaatgcggc tacaccgaac
     5581 agtaccgatg aaacaccttc cagccagact aacggtttgc tgttcatttg cggtaattca
     5641 ttagctacgc tggcgtccac caactggctg gggaatttgc ttttcaacag catcagcgtg
     5701 atcagtacaa acagaatacc cggaataatc agcccatagc cgtaccagat attattgagc
     5761 aacatatagc tcaccagcat tgggtagaac atttgcccaa atgacaccat cgctttaacc
     5821 agtatgaccg ccgaaccaga ggctttcgga aagcattcca tgagcgcggg gtagccaccc
     5881 gtatccagcg ctgagttagc gatacctacg cacactgcca gaccgtaggc gagagttaaa
     5941 ttcgggcaag cgggaatacc aaagaagaat agcagataca ttattactgc cattaatatc
     6001 accgcccgac gaccaaactt atcggagatc acaccgaaga ataaaatact gatcaatcgc
     6061 cccaaaccga taccggaaat taagtaggca atgcccgcgt tgtcagtgga aaacttttcc
     6121 gccagagatg acatattttg ggcaagcgta ataacactaa tgccgtgcag gaagtagctg
     6181 aagtaaatac aaagaacagc caggataaat ggcgtgctga aagccttatt ttgagacatt
     6241 ttcacagctc cttgctgaaa gataatttcg ttaccgtacg tattgttctc cgacggttta
     6301 cataattact atactgaaga tgattttccc tgggtacatg gcataaagtt tatgtttcca
     6361 ggaatattgc atgtcatagt ttgtttttga tcgcgatttt ccttagttag cgcagaaaga
     6421 gttatttatc cgtaagatct tgcatgacct gaaaagaata ttgcattacc cacccggagc
     6481 gacattttcc tttagcgagt gatgccgcgt ttttttgcgg ctgcgtaagc cgataaacag
     6541 tgccagtaaa aaaccgatgg cagcgatggc ggtatcgaac cacataatat cggcaatact
     6601 tctttgggac agatgagccg taatcaacgg aatagtaaag gtcgcaatac tgcccgcact
     6661 gtaatagatc cctgtagctt tacctttagc gtaagggaaa cgttcagcca ttaacgtcag
     6721 gccaatttgc acaacacctc cagcagaagt aaaaccaatg acaaaagaaa atattatcac
     6781 cacataaaat gtgggatgca ggcagacggt aaacagagca ataaatgaga taaaggtgta
     6841 cagcatcagt aatgttgttg ggcgaacggt attacgaatg agtggagcgg taataaatac
     6901 acaaagcagc gaacccacgg tgtagatact gagtagtttg atcgacatag tgtatgacat
     6961 gcctgcaaca aattgtccgt actgtgccag ccactggcta accagataaa acgttgccat
     7021 tgagatatag ccatataagg tataactggc taaatcgata attgaacagc gatgttccgt
     7081 agagctggtg gttttcttta tgacaggtaa gcgacgaccc ggatggggtg ggaacgtaca
     7141 acggtataaa aacagagcgt taataaacat aatgcctgca gcaatcataa aggaccaacc
     7201 gaaccacagt tcagcccaca ctaacaggct aatgattagc ggtaataaaa attgtccgct
     7261 ggaaacaaat gctttaatta aaatattggc tgtcccaggt gagcgtggaa aagcttccat
     7321 caaactggga taagtgcctg catcgagaaa actgtttgcc attcccgcca gaaagccaaa
     7381 aacataagcg ataatgatgt tattggtctg caggatgcca aaaaagaagg ccatatagca
     7441 gcacatcccg agcatgataa aagggcggcg accaaagcga tcggataata atcctgcaaa
     7501 aagcaagaca cttaatcgac caatgcccag cgatgagata actatcgaga cacccgcggc
     7561 attagactgc caaagtgtct ccagcgaggc catattcagg ctcatcaaaa tgacgcccat
     7621 accatgcacc aggtaattaa aatacaaccc aagtgcggta gggtagtagg gatttttcat
     7681 aggttctact tagcaaaggt gctttaaaaa gtgtagcgat gttgtgtcag tatccgttca
     7741 ttattgttgt aacaacgaca attccttttt gtggagaaat taacaaagcc gctccagatc
     7801 gtgtgcatat aattcagcgg ctaccgattg atagatcttc cagtcaataa aattgccatc
     7861 agggtagact tgttgaaacg cggttaagtc ggggaaaaaa cgcatggtgt tgttgccgat
     7921 gcggttattt attttctcta tgatcgcatg aagatgtttt tttctttgct gacgcatagc
     7981 caacagttgc tcgactacac aatcaggaat ggcacacctg ccattttccc attgacgcca
     8041 gctttcactg tcaccggtct gggcaatcca ggtcgcgcat tcatcaatag tcatggcaaa
     8101 aatatggcgg agtgcctgga gttcataagc gttcatcgtg taatggctgc atgaaataaa
     8161 caataaaata taaataacat tatcagcctg ctgacggctt gaagagagtc acagtatctt
     8221 gtgcaacatt attggaaaga gtgaggggcg aggtagcgaa gcggaaaaat gttaaaaaaa
     8281 agcccatcgt gggagatggg caaagactac acacagcaat tcgttgtttc actcagggga
     8341 tttccatgct tataaatcaa tatgttgatt tataaccgtg agctaatagt aggcatacgg
     8401 actttttgcg tcgatcagat tcgtctcatt agtttaaaaa ctgtaaagaa tttgcgacag
     8461 gtactaccag ctgaatggcg gtaagcagga aaatatgggg aagagcaagc aaatatcaat
     8521 tagcggtggt taacaatcag gcggcagagc cgcctgatga gaaattactt attcggtttc
     8581 tggatttcgc caagttcttc gagaatttct tccggttgat ccgtcggcgg tgggacctct
     8641 tccacccacg cggcaaacag acgccaggaa acggctaaca gaaccggacc aataaacaga
     8701 ccgatcatcc cgaaagcaat caaaccacca ataacgccag agaggatcag gatcagcggt
     8761 aaatcggcac ccatgcgaat taacattggg cggatgacgt tatccagcgt gccaaccaca
     8821 ccgctccaca ctaacaatac cgttccccag gtggtatcgc cagtccagta gagccagata
     8881 atcgccggaa tcagtaccgg caacgggcca agctggacaa ggcaggaaag gatcattaac
     8941 accgttagca aagttgcata aggtacgccg gatacggcga ggccgatacc gccaagcact
     9001 gcctgtacta acgccgtcac caccacaccc agcgccaccg cgcggatagc ctgtgccgcc
     9061 agcagtacgg cggcatctcc gcgaacgcct gccagacggg tcgcaaaatg gcgaatgcct
     9121 tgtgccacct gttcaccgcg ccagtacagc agggcactga agagaagcat cagcgcacaa
     9181 tgcaccataa agcgcccgat atgcgccgct tgcccaacga accaggtagt ggtggtgcca
     9241 atataagggc ggactttcgc catgatcgcc gtccccccca tatccagcaa gttgtgccag
     9301 cctgcataca gcttcgcgcc aatcaccgga atggtattaa gccacgccag atcgggtaac
     9361 gtcatgtcac cgctggaaat ggctttaatt agcgggccgc tgccgtcgac gatactgtta
     9421 accagcaaag caatagggat gataaacacc atcactaata acagcgtcat caccagaacg
     9481 gcgagagagc ggcggccaaa catgatcttt tgcaaacgta acaataccgg ccaggtggcg
     9541 ataaccaccg taccggccca tgcaaagccg agaataaagg gttgaacaat ccacagacat
     9601 gccacaatca tgatggctaa aaacagcacc gaaagcagaa tttgtgcgac atccctgggc
     9661 tgacgaacat ttaccataac tacttttcac cttttttgtg cgtcaaatcg tcgacgcgac
     9721 gtgaacacgc gaaactcacc tgcataatga tgagcaattt cagcggtttt taacaggccg
     9781 attctgccct taattctgat gggcgtgtta gaaaaaaatg tggtacaaca actcaggcaa
     9841 cacgcaaacg atttactcgt cgtatttcaa gttgcatatg ctgcggctac gttcgttcac
     9901 cccagttact tactgttgta agctcctgga gttcactcgc ttgccgcctt cctgcaactc
     9961 gaaatccata gagtaaataa acatcacctt tctgtgcgct attgcagccg cctgaaagaa
    10021 cagtgtatcg acctacatca cagaccgcag gaaagggtca atataatgat tccacagatt
    10081 tcccaggcac ccggcgtcgt tcaactggtg cttaattttt tgcaagagct ggagcaacaa
    10141 ggttttaccg gcgatacggc gacaagttat gctgatcgtc tgacaatgtc gaccgacaac
    10201 agtatttacc aacttctccc cgatgcagtg gtatttccgc gttcaaccgc agatgtggcg
    10261 ctgatcgccc gtcttgccgc gcaggaacgc tattcatcgc tgatctttac cccccgcggc
    10321 ggcggcaccg gcactaacgg tcaggcgctc aaccagggta ttattgttga tatgtcccgc
    10381 catatgaacc gcatcatcga aattaaccct gaagagggct gggtgcgcgt tgaggccggg
    10441 gtgataaaag accaactcaa tcagtacctg aaaccgttcg gctacttttt tgcaccagaa
    10501 ctttcgacca gcaaccgggc aacgctcggc gggatgatca ataccgatgc atccggtcag
    10561 ggatcgctgg tctatggcaa aacgtcagat cacgtacttg gcctacgtgc ggtattgttg
    10621 gggggcgata ttctcgatac gcaaccttta cccgtcgagc tggcggaaac gctgggtaaa
    10681 tccaatacca caatcgggcg aatttataac acggtttatc aacgttgccg tcagcaacgc
    10741 cagttaatca tcgacaactt ccccaaactt aaccgctttc ttaccggtta cgatctgcgt
    10801 catgtcttta acgatgagat gaccgagttc gatctgacgc gcattctgac gggttcagaa
    10861 gggacgctgg cctttattac cgaagcgcgg ctggatatta cgcgcttgcc taaagtgcgc
    10921 cgtctggtga atgtcaaata tgactctttt gactctgcgc tgcgtaacgc gccgtttatg
    10981 gttgaggcgc gggcgctttc ggtagagacg gtggactcaa aagtgctgaa tctggcgcgg
    11041 gaagatattg tctggcattc cgtcagcgag ttgattaccg atgtgcctga caaagagatg
    11101 ctcgggctga acattgtgga atttgctggt gatgatgagg cgctgattga cgagcgggta
    11161 aatgcactct gtgcgcggtt ggatgagctg atcgccagtc atcaagcagg tgtcatcggc
    11221 tggcagatgt gccgtgagct ggcgggggtt gaacgtatct atgcaatgcg caaaaaagcc
    11281 gttggtctgc ttggcaatgc caaaggtgcc gctaagccaa ttccgtttgc tgaggatacc
    11341 tgcgtaccgc cggaacatct ggccgattat attgctgaat ttcgcgcgct gctcgacagc
    11401 cacggcttaa gctacggtat gttcggtcac gtcgacgcag gtgtcttgca tgtccgtccg
    11461 gcactggata tgtgcgatcc gcaacaagag attttgatga agcaaatctc tgatgacgtg
    11521 gtggcgctga ctgcgaaata cggtggtttg ttgtggggcg agcacggcaa aggttttcgc
    11581 gctgaataca gcccggcgtt tttcggtgag gaactttttg cagaactgcg caaagtgaaa
    11641 gcggcatttg acccgcataa ccgactcaac ccagggaaga tttgcccgcc agaaggtctc
    11701 gatgcgccga tgatgaaagt ggacgcggtg aagcgcggta cattcgatcg gcagatcccc
    11761 attgcggtac gccagcagtg gcgcggtgcg atggagtgta acggcaacgg tttatgcttc
    11821 aactttgatg cccgtagtcc gatgtgtccg tcgatgaaga tcacccagaa ccggattcat
    11881 tcaccgaaag ggcgcgcaac gctggtgcgt gaatggctgc gtttgttggc ggatcgcggc
    11941 gttgatccac tcaaactgga acaagaactg cctgaatccg gcgtcagctt gcggacgtta
    12001 attgcccgca cgcgcaatag ctggcatgcg aataaaggcg aatatgactt ctcacacgaa
    12061 gtcaaagagg cgatgtcggg ctgtctggcc tgtaaagctt gttcgaccca gtgccccatc
    12121 aaaattgatg tgccggagtt tcgctctcgt tttctgcagc tctatcacac ccgttattta
    12181 cgcccgctgc gcgaccacct cgtcgctacg gtcgagagct acgcgccgct gatggcacgc
    12241 gcgccgaaaa cctttaactt cttcattaac cagccgctgg tgcgcaaact ctcggaaaaa
    12301 catatcggca tggttgattt gccgctgctg tcggtcccct cgctacaaca acaaatggtg
    12361 gggcatcgct cggcaaacat gacgctggaa cagcttgaat ctctcaatgc agagcagaaa
    12421 gcgcgcacag tgttggtggt gcaggacccc tttaccagct attacgatgc gcaagtggtg
    12481 gcggattttg tccgtctggt cgaaaaatta ggtttccagc ctgtgttact gccattttcg
    12541 ccaaatggca aagcccagca tattaaaggt tttcttaatc gttttgcgaa gacggcgaaa
    12601 aagacggcgg atttcctcaa ccgtatggcg aagctgggta tgccaatggt gggcgtcgat
    12661 ccggcgctgg tactttgtta tcgcgatgaa tataaactgg cgctgggcga ggagcgtggc
    12721 gagtttaacg tcttactggc gaatgaatgg ctggcaagcg cacttgagtc acagccagtg
    12781 gctacagtca gcggtgaatc atggtatttc tttggtcact gtaccgaagt taccgccttg
    12841 ccgggtgcgc cagcacaatg ggccgcgata tttgcccgtt ttggcgcgaa actggaaaat
    12901 gtcagcgtgg gttgctgcgg catggcaggg acttacggac atgaagcgaa aaaccataaa
    12961 aattcgctcg ggatctatga gttatcctgg catcaggcta tgcagcgact gccgcgtaac
    13021 cgctgtctgg cgaccggata ttcctgccgt agtcaggtca aacgggttga aggcacgggg
    13081 gtacgccatc ctgtgcaggc tttactggag attattaaat gatatggaaa cgaaaaatcc
    13141 ccctggaagc actgaatgcc atgggtgaag gaaacatggt ggggttactg gatattcgct
    13201 ttgaacatat tggtgatgac acccttgaag cgacaatgcc agtagactcg cggacaaagc
    13261 agcctttcgg gttgctacat ggaggtgcat ccgtggtact ggccgaaagt atcggttccg
    13321 ttgccggtta tttatgtacc gaaggtgagc aaaaagtggt tggtctggaa atcaatgcta
    13381 accacgtccg ctcggcacga gaagggcggg tgcgcggcgt atgcaaaccg ttgcatctcg
    13441 gttcgcgtca ccaggtctgg cagattgaaa tctccgatga gaaagggcgt ttgtgctgtt
    13501 cgtcacgatt gacgaccgcc attttgtgac gttgtcacca gaaaagtgtg acgcatattg
    13561 cttttggtca ttatctgcac agtaaacggt ggtatactgt gcaggtcttg tctaaaagcg
    13621 aggatgctat gtctactcaa cttgatccca cccagctggc cattgaattt ttacgccgtg
    13681 accagagcaa tctttcacct gcccaatatc ttaaacgtct gaaacaacta gaacttgaat
    13741 ttgctgacct gcttactctc tcttctgctg aattaaaaga agaaatctac tttgcctggc
    13801 gtctgggcgt gcattgatcc tgacttaatg ccgcaagttc gccaaatcga cctggattaa
    13861 cctccgtcca ggttcgatga ccttcttaat actgcttatc tacccgcctg atattgttca
    13921 aaaataatac taatcgcagt ttgtgttaaa acggcggctt agctttatga gattattctt
    13981 atcgcccctt caagagctaa gccactgaga gtgccggaga taagcgccgg atggggtaga
    14041 aacccttaag cctgtgtcgc acagacttaa gggttttctt atttctgaac gctgcacttt
    14101 tttacctatc tcttaaatag tcattttcaa tacaactttt tctttttcat cacagcctta
    14161 atgctcctta gataataatc attatctaac aatgagatac ctaattctta gcgtgcctgt
    14221 taacccacac atcagggtct atgcttatta aaagcaggca aaaagcatgt tttacggtaa
    14281 agcccctgcc tttgctgggt tgaactgata atcattatca ctaacatgct gttatacgct
    14341 gaaagcgatg aagtgaggta aatcgatgga catgcattca ggaaccttta atccacagga
    14401 tttcgcctgg caaggcttaa cgctgacacc cgcagccgct gtccacatcc gcgagctggt
    14461 ggcaaagcag ccgggtatgg tcggcgtgcg cttaggtgtg aagcaaacgg gctgcgcggg
    14521 ctttggctat gtgctcgaca gtgttagcga gccggacaaa gacgatctgc tgtttgaaca
    14581 cgacggcgcg aagctgtttg tcccgctgca agcgatgccg tttattgatg gcacggaagt
    14641 cgatttcgtt cgtgaaggac ttaatcagat attcaaattt cacaacccta aagcccagaa
    14701 tgaatgtggc tgtggcgaaa gctttggggt ataggcggta ctatgtctcg taatactgaa
    14761 gcaactgacg atgtcaaaac ctggaccggc ggcccgctga attataaaga aggattcttc
    14821 acccagttag ccaccgatga gctggcaaag gggataaacg aagaggtggt gcgcgcaatt
    14881 tcggcgaagc gtaatgagcc ggagtggatg ctggagtttc gtctaaacgc ctatcgcgca
    14941 tggctggaga tggaagaacc gcactggttg aaagcgcact acgacaagct gaattatcag
    15001 gattacagct actactcggc accatcgtgc ggtaattgtg acgacacttg cgcgtctgaa
    15061 cctggcgcgg tgcagcaaac tggcgcgaac gcctttttaa gtaaagaggt ggaggcggcg
    15121 tttgagcagt tgggtgttcc cgtgcgggaa ggcaaagagg tggcggtgga tgccattttc
    15181 gactcagttt cggttgccac tacttatcgc gaaaaactgg cggagcaggg aattattttc
    15241 tgttcctttg gtgaggcgat ccacgatcac ccggaactgg tgcgtaaata tctcggcacc
    15301 gtggtgccgg ggaatgacaa cttctttgcc gcgcttaatg cggcggtagt ctctgacggt
    15361 acgtttattt atgtgcctaa aggcgtgcgc tgcccgatgg aactttccac ctattttcgc
    15421 attaacgcgg aaaaaaccgg acagtttgag cgcaccattc tggtggccga cgaagacagc
    15481 tacgtcagct acattgaagg ctgttccgct ccggtgcgtg acagctatca gttgcacgcg
    15541 gcagtggtgg aagtcatcat ccataaaaac gccgaggtga aatattccac ggtacaaaac
    15601 tggttccctg gcgataacaa caccggcggt attctcaact tcgtcaccaa gcgtgctttg
    15661 tgcgaaggcg aaaacagcaa aatgtcatgg acgcaatcag aaaccgggtc agcgattacg
    15721 tggaaatatc ccagctgcat tttgcgcggc gataactcca ttggtgagtt ttactcagtg
    15781 gcgctgacca gcggtcatca gcaagcggat accggcacca agatgatcca catcggtaaa
    15841 aacaccaaat cgaccattat ctcgaaaggg atctctgccg gacatagcca gaacagttat
    15901 cgcggcttag tgaaaatcat gccgacggca accaatgcgc gcaatttcac tcagtgcgac
    15961 tcaatgctga ttggcgctaa ttgtggggcg cataccttcc cgtatgtcga atgtcgcaat
    16021 aacagcgcac aactggagca cgaggcaacg acatcacgta ttggtgaaga tcaactgttt
    16081 tactgcctgc aacgcgggat cagcgaagaa gacgccatct cgatgattgt taacggtttc
    16141 tgcaaagacg tgttctcaga actgccgctg gaatttgccg ttgaagcaca aaaactcctc
    16201 gccatcagtc ttgaacacag cgtcggataa ggaataaaca tgttaagtat taaagattta
    16261 cacgtcagcg tggaagataa agctatcctg cgtggattaa gcctcgacgt tcgtcccggc
    16321 gaagttcacg ccattatggg gccaaacggt tcgggcaaaa gtaccttatc ggcaacgctt
    16381 gccgggcgag aagattatga agtgacgggc ggcacggttg agttcaaagg caaagatttg
    16441 cttgcactgt cgccggaaga tcgtgcaggc gaaggcatct ttatggcctt ccagtatccg
    16501 gtggagattc caggtgtcag taaccagttt ttcctgcaaa cggcacttaa tgcggtgcgc
    16561 agctatcgcg gccaggaaac gctcgaccgc tttgattttc aggatttgat ggaagagaaa
    16621 atcgctctcc tgaagatgcc ggaagattta ttaacccgtt cggtaaacgt tggtttttcc
    16681 ggcggcgaga aaaagcgcaa cgatattttg caaatggcgg tgctggaacc ggagttatgc
    16741 attcttgatg aatcggactc cgggctggat atcgacgcat taaaagtggt cgccgatggc
    16801 gtgaactcgc tgcgtgatgg caagcgctca ttcatcattg ttacgcacta ccaacgcatt
    16861 ctcgactaca tcaagcctga ttacgttcat gtgctatatc agggacgaat tgtgaaatcc
    16921 ggcgatttca cgttggtcaa acaactggag gagcagggtt atggctggct taccgaacag
    16981 cagtaacgcg ctgcaacagt ggcatcactt gtttgaagct gaagggacaa aacgctcccc
    17041 gcaagcacag cagcatttac aacaattgct gcgtaccgga ctgccgacac gtaaacatga
    17101 aaactggaaa tatacgccgc tggaagggct gaccaatagt cagtttgtca gcattgcggg
    17161 agagatatcc ccacagcagc gagatgcctt agcgttaacg ttagacgctg tgcggctggt
    17221 atttgtcgat ggacgttacg tgcctgcgct gagcgatgcg actgaaggca gcgggtatga
    17281 agtgagcatt aacgacgacc gtcaggggtt acccgatgct attcaggcgg aagtgtttct
    17341 gcatttgacg gaaagcctgg cacaaagcgt gacgcatatc gccgtgaagc gcggtcaacg
    17401 gccggcaaag ccattgctgt taatgcatat cacccagggc gtggcaggtg aagaggtgaa
    17461 cactgcccat taccgacatc atctggagct ggcggaaggt gccgaagcaa cggtgatcga
    17521 acattttgtc agccttaatg atgctcgtca ttttaccggg gcacggttct ctatcaacgt
    17581 cgcagcgaat gcccacttgc agcatatcaa gctggcgttt gaaaacccgc tcagtcacca
    17641 ctttgcccat aacgatttgt tgctggctga cgatgccacc gcatttagcc acagtttcct
    17701 gctgggtggc gcagtgttac gacacaacac cagtacgcaa ctcaatggcg aaaactgcac
    17761 gctgcggatc aatagcctgg cgatgccggt gaaaaacgag gtgtgtgata cccgtacctg
    17821 gctggaacac aataaaggtt tttgtaacag ccgacagttg cacaaaacta tcgtcagcga
    17881 caaaggccgc gcggtattta acggtttgat caacgtcgcg cagcacgcca tcaaaacgga
    17941 tggtcagatg accaacaata atctgctgat gggcaaactg gcggaagtgg atacgaaacc
    18001 gcagctggaa atctatgcag atgatgtgaa atgcagccac ggcgcgacgg tggggcgtat
    18061 tgatgatgaa cagatgttct atctgcgctc gcgcgggatc aatcagcagg atgcccagca
    18121 gatgatcatt tacgcctttg ctgccgaact gacggaagca ctgcgtgatg aggggcttaa
    18181 acagcaggtg ctgacccgaa tcggtcaacg gctgccagga ggtgcaagat gactttttcc
    18241 gtcgacaaag tgcgggccga ctttccggtg ctttcgcgtg aggtaaacgg tttgccgctg
    18301 gcttatctcg acagcgccgc cagtgcgcag aaaccgagcc aggtgattga cgccgaggcc
    18361 gagttttatc gtcatggcta cgcggcggtg catcgcggta ttcatacctt aagcgcccag
    18421 gcgaccgaga aaatggagaa cgtgcgcaag cgggcatcgc tgtttattaa tgcccgttcg
    18481 gcggaagagc tggtgttcgt ccgcggcacg acggaaggga tcaatctggt cgccaatagc
    18541 tggggcaaca gcaacgtgcg ggcgggcgat aacatcatca taagtcagat ggagcaccac
    18601 gctaacattg ttccctggca gatgctttgc gcacgcgttg gcgcagagct gcgtgtgatc
    18661 ccgctcaatc ccgacggtac gttgcaactg gagacgctgc ctacgctgtt tgatgagaaa
    18721 actcgcctgc tggcaattac tcatgtctcc aacgtgcttg gcacagaaaa tccactggcg
    18781 gaaatgatca cgcttgcgca ccagcatggc gcaaaagtgc tggtggatgg cgctcaggcg
    18841 gtgatgcatc atccggtgga tgttcaggcg ctggattgcg atttttacgt gttttccggg
    18901 cataaactgt atggccccac cgggattggc attctttatg tcaaagaagc cttgctacag
    18961 gagatgccgc cgtgggaagg gggcggttct atgatcgcca ccgtcagcct gagtgaaggc
    19021 actacctgga ccaaagcacc gtggcggttt gaagccggta cacccaatac cgggggcatc
    19081 attggtcttg gcgcggcgct ggagtatgtt tcggcgcagg ggcttaataa catagccgag
    19141 tatgaacaga atctgatgca ttacgcgcta tcacagctgg aatctgtacc ggatctcact
    19201 ctctatggcc cacaaaacag gcttggcgtt attgctttta atctcggtaa acaccacgcc
    19261 tatgatgttg gcagttttct cgataattac ggcattgctg tgcgtaccgg acatcactgc
    19321 gctatgccat taatggccta ttataacgtc cctgctatgt gtcgggcgtc gctggccatg
    19381 tataacaccc atgaagaagt ggatcgtctg gtgaccggac tgcaacgtat tcaccgtctg
    19441 ctgggataac agggaggcac tatggcttta ttgccggata aagaaaagtt gctgcgtaat
    19501 tttttacgct gcgccaactg ggaagagaaa tatctctaca ttattgagct gggccagcgt
    19561 ctgccagaat tacgcgacga agacagaagc ccacaaaata gcattcaggg ctgccagagt
    19621 caggtgtgga ttgtcatgcg tcagaatgcg cagggaatta ttgaattgca gggcgacagc
    19681 gatgcggcga ttgtgaaagg gcttattgcg gtcgtcttta ttctctacga tcagatgacg
    19741 ccgcaggatg ttgtcaattt cgatgtgcgt ccgtggtttg aaaaaatggc actcacccaa
    19801 catctcaccc catctcgttc acaaggtctg gaagcgatga ttcgcgcaat tcgcgccaaa
    19861 gccgctgcac ttagctaaac taaagggaca gctttcatcc ggttggctca cattgccgga
    19921 tggtcaatgt ttacactgac ctgcacagga gtgtttcggt ttcaggtgaa cataagcgcc
    19981 atccgacgtt tcagcgtgag tctccggcaa atacaggagg tttacaatga aacgcgcgtc
    20041 tttgcttaca ctcacgctta tcggcgcttt tagtgccatc caggctgcct gggcggttga
    20101 ttatccgcta ccaccaaccg gaagccgact ggttgggcaa aatcaaacgt atacggtgca
    20161 ggaaggggat aaaaaccttc aggccatcgc ccgacgtttt gatactgcgg caatgttgat
    20221 ccttgaagcc aataacacta tcgccccggt gccaaaaccc ggtacgacga taactattcc
    20281 ttcgcaactg ttattacctg atgcgccgcg tcaggggatt atcgttaacc ttgcagagct
    20341 gcgcctttat tattatccgc cgggagaaaa tatcgtgcag gtttatccga taggtattgg
    20401 cttgcagggg ctggaaacgc cagtgatgga aacgcgtgtg gggcagaaaa tccctaaccc
    20461 aacctggacg cctacggcag gcattcgtca acgttcgctg gagcgtggca ttaaattacc
    20521 gccagtcgtt cctgccggac caaataaccc gctaggacgt tacgcactgc gcctcgcgca
    20581 tggtaatggc gaatacctca ttcatggcac cagtgcgccg gacagcgtcg gtttgcgcgt
    20641 cagttcaggg tgtattcgca tgaatgcacc ggatattaaa gccttgttct ccagcgtgcg
    20701 gacgggaacg ccggtgaaag tgatcaacga accagtgaaa tattccgtgg agcctaacgg
    20761 gatgcgttat gttgaagtac atcgaccact atcggcagaa gaacagcaga acgttcagac
    20821 aatgccatac acactgccag caggctttac gcaatttaaa gacaataagg ctgtagatca
    20881 gaagttagtc gataaagcgt tgtatcgtcg ggcagggtat ccggttgcgg tgagcagtgg
    20941 agcaactccc acagccagca atgcgccttc agtagagtca gcgcagaatg gtgaaccaga
    21001 gcaagggaat atgttacgcg cgacgcagta gtggtaaata gcagacaaaa aaatggcgca
    21061 caatgtgcgc catttttcac ttcacaggta ctattacttg cggtatttag tagccatgtt
    21121 gtccagacgc tggttagcac gagctgcgtc atctttagca gcctgaacgt cggaacgcat
    21181 tgcgttcacg tcgttgctca gctggtcaac tttagcgttc agagtctgaa cgtcagaaga
    21241 cagctgatcg attttagcgt tgctggagca acctgccagc agagtagaac ccaggattac
    21301 cgcgcccagt accagtttag tagctttcat tattaatacc ctctagattg agttaatc
//
LOCUS       DBFIFM010000067        20811 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.067, whole genome shotgun sequence.
ACCESSION   DBFIFM010000067 DBFIFM010000000
VERSION     DBFIFM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.067"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(24..1673)
                     /gene="actP"
                     /locus_tag="N7C74_002168"
     CDS             complement(24..1673)
                     /gene="actP"
                     /locus_tag="N7C74_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HGU2432033.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1670..1984)
                     /gene="yjcH"
                     /locus_tag="N7C74_002169"
     CDS             complement(1670..1984)
                     /gene="yjcH"
                     /locus_tag="N7C74_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HGU2432034.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2185..4143)
                     /gene="acs"
                     /locus_tag="N7C74_002170"
     CDS             complement(2185..4143)
                     /gene="acs"
                     /locus_tag="N7C74_002170"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HGU2432035.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLKLGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDQVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDS
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4536..5972
                     /gene="nrfA"
                     /locus_tag="N7C74_002171"
     CDS             4536..5972
                     /gene="nrfA"
                     /locus_tag="N7C74_002171"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HGU2432036.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSHDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6017..6583
                     /gene="nrfB"
                     /locus_tag="N7C74_002172"
     CDS             6017..6583
                     /gene="nrfB"
                     /locus_tag="N7C74_002172"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HGU2432037.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6580..7251
                     /gene="nrfC"
                     /locus_tag="N7C74_002173"
     CDS             6580..7251
                     /gene="nrfC"
                     /locus_tag="N7C74_002173"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HGU2432038.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7248..8204
                     /gene="nrfD"
                     /locus_tag="N7C74_002174"
     CDS             7248..8204
                     /gene="nrfD"
                     /locus_tag="N7C74_002174"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HGU2432039.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLSLVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLAAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LFKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8224..9942
                     /gene="nrfE"
                     /locus_tag="N7C74_002175"
     CDS             8224..9942
                     /gene="nrfE"
                     /locus_tag="N7C74_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HGU2432040.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWKLKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWQYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALLAHAGILLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDV"
     gene            9935..10318
                     /gene="nrfF"
                     /locus_tag="N7C74_002176"
     CDS             9935..10318
                     /gene="nrfF"
                     /locus_tag="N7C74_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HGU2432041.1"
                     /translation="MYKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10315..10911
                     /gene="nrfG"
                     /locus_tag="N7C74_002177"
     CDS             10315..10911
                     /gene="nrfG"
                     /locus_tag="N7C74_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HGU2432042.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCIGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQNPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11253..12566
                     /gene="gltP"
                     /locus_tag="N7C74_002178"
     CDS             11253..12566
                     /gene="gltP"
                     /locus_tag="N7C74_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HGU2432043.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12744..13433)
                     /gene="yjcO"
                     /locus_tag="N7C74_002179"
     CDS             complement(12744..13433)
                     /gene="yjcO"
                     /locus_tag="N7C74_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HGU2432044.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13527..15674)
                     /gene="fdhF"
                     /locus_tag="N7C74_002180"
     CDS             complement(13527..15674)
                     /gene="fdhF"
                     /locus_tag="N7C74_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15255..15257),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase N subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HGU2432045.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLSIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15872..17338)
                     /gene="mdtP"
                     /locus_tag="N7C74_002181"
     CDS             complement(15872..17338)
                     /gene="mdtP"
                     /locus_tag="N7C74_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HGU2432046.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVTAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGSGYQAAPVVEKK"
     gene            complement(17335..19386)
                     /gene="mdtO"
                     /locus_tag="N7C74_002182"
     CDS             complement(17335..19386)
                     /gene="mdtO"
                     /locus_tag="N7C74_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HGU2432047.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAITEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIHQGRDLLHAWDATWNSAQALDNALQPDKAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19386..20417)
                     /gene="mdtN"
                     /locus_tag="N7C74_002183"
     CDS             complement(19386..20417)
                     /gene="mdtN"
                     /locus_tag="N7C74_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HGU2432048.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYV
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKSEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20436..20711)
                     /gene="ytcA"
                     /locus_tag="N7C74_002184"
     CDS             complement(20436..20711)
                     /gene="ytcA"
                     /locus_tag="N7C74_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HGU2432049.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANIKLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4692 a         5506 c         5689 g         4924 t
ORIGIN      
        1 agacgaccgg ggaaggggag agattaatgc gcgcggcctt gctcaacgcc aaagccggtc
       61 tgggagcgga taaactgcgc gcggaacagt tcacgctcac gcgcgccttc cgctgagtta
      121 tcggttgccg agaagaacca gatgccgagg aatgccacgg tgatagagaa cagcgccggg
      181 tattcatacg ggaagatggc tttttcgtga ccgaggatct gtacccaaat cgtcgggccg
      241 aggatcatca gtaccactgc ggtaatcagc cccagccagc cacccatcat cgcgccacgc
      301 gtggtcagtt tcgaccagta catagaaagc agaatgatcg ggaagttaca gctcgccgcg
      361 atggcaaacg ccagccccac cataaaggcg atgttctgat tctcaaacag cacgccgagg
      421 ataatcgcaa tcacacccaa aatcaggacg gtgattttcg atacccgcag ctcttcacgt
      481 tcggtcgcgc cttttttgaa gacgttagcg tacaggtcat gcgaaaccgc tgatgcgccc
      541 gccagcgtca gacccgcaac caccgcgagg atagtggcga aagcaaccgc tgaaataaag
      601 ccgaggaaca ggttgccgcc caccgcattg gccaggtgaa ctgctgccat gttgttacca
      661 ccaatcagat ggcccgccgc gtctttatat tccggattcg caccgaccag catgatcgcg
      721 ccgaagccga taataaaggt caggatatag aagtagccca taaatccggt agcgtagaac
      781 acgcttttac gcgcttcgcg ggcatcgctg accgtaaaga agcgcatcag aatgtgcggc
      841 aagccagccg taccaaacat cagtcctagc cccagagaga gtgcggagat cggatctttc
      901 accagtccgc ccggtttcat gatgtcgaca cctttcgggt gtaccgccat cgcttcgctg
      961 aacagattgt tgaagctaaa gccgacgtgt ttcatcacca taaaggccat aaagctggca
     1021 ccgaacagca acagcacagc tttgataatc tgtacccagg tggtcgccag catgccgccg
     1081 aacaggacgt acatcatcat cagcacgccg accagcacca ctgcaatgtg atagttaagg
     1141 ccaaacagca gctcgatcag tttacctgcc cccaccatct gggcgataag gtaaagcgcc
     1201 accaccacca gagaaccaca ggccgaaaga atacgaatcg gcccttgttt cagacgataa
     1261 gaggctacat cggcaaaggt gtaacgcccc aggttacgca gacgttcggc aatcaggaac
     1321 aaaatgatcg gccagcccac caggaagccc agcgagtaaa ttaagccgtc atagccggag
     1381 gtaaacacca gtgcggagat ccccaaaaat gaggcggcgg acatatagtc accggcaatc
     1441 gccagcccgt tctgaaaacc ggtgatattg ccgcctgcgg tgtaatagtc attacgcgaa
     1501 cgtacgcgtt ttgacgccca gtaggtaatg ccgagcgtaa acacgacgaa aatcaggaac
     1561 ataataatcg cctgccagtt cgttggctgg cgctctacgg ccccgctaat agcatccgcg
     1621 gcgttagctg cgaaagggag tgtggcggca agcgccgtca gaactctttt catgatgctt
     1681 gtacctcatg caggacttca ttattaagac ggtcgaattc gccgttcgcc cgccagatgt
     1741 agataccagt gagaacaaag gagatcacta ttaccccaac accgactgga ataccgcgtg
     1801 tgacgctggt gttcggattc agcggggtgc ccagccagcc gggcgcgaag gcgatcagta
     1861 aaataaagcc gatataaact gccagcataa taatcgacag gatggtggca aaccgttgcc
     1921 gtttttcgac taactccctg aaatgcgcat tgtcttctat ccgctgataa atagtgccat
     1981 tcatcacaga ttctccagag gtaatgtagg gattgtttta attcccgctc ccttatggga
     2041 gaaggttaac gctcgggtaa cccttgccga atgtaggccg gataaggcgt tatacgccgc
     2101 atccggcaat caatgcctga tgcgacgctg tcgcgtctta tcaggcctac aaaccgttac
     2161 cgactcgcat ccggcaattg tgggttacga tggcatcgcg atagcctgct tctcttcaag
     2221 cagcttctcg actacgccag gatcggcaag cgtcgaggta tcgcccaggt tgctggtatc
     2281 gcccgccgca attttgcgca gaatacggcg cataattttg ccggagcggg ttttaggcag
     2341 ggagtcggtc cagtgcagca cgtctggcgt cgccagcggg ccaatctctt tacgcaccca
     2401 gttgcggact tctgcgtaca gttctggtga cggttcctcc ccgtgattaa gcgtgacgta
     2461 ggcgtagatc gcctgacctt taatattgtg cggaataccg acgacagcgg cttcggcgat
     2521 tttcggatgc gccaccagcg ccgactcaat ctccgccgtt cccagacggt gaccggagac
     2581 gttcagcaca tcgtccacac gcccggtgat ccagtaatag ctatcttcat cacgacgcgc
     2641 gccgtcgccg ctgaaataca tatttttgaa ggtggaaaaa taggtctgct caaaacgttc
     2701 gtgatcgcca aacagcgtac gcgcctgacc cggccaggag tcggtgatca ccaggctacc
     2761 ttcggtagcc ccttccagcg ggttaccttc gttatcgacc agcgccggtt gcacgccgaa
     2821 gaacggacgt gttgccgaac cggctttcag ctcggtagcg ccaggcagcg gggtgatcat
     2881 gaaaccgccg gtttcggtct gccaccaggt atcgaccacc ggacatttct cgttgccgat
     2941 ttttttccag taccactccc acgcttccgg gttaattggc tcgcccacgg aaccgagaat
     3001 gcgcagcgac gaacggtcgg tgccttcgat cgctttatcg ccttccgcca tcagcgcgcg
     3061 aatcgccgtg ggcgcggtat agagaatatt gacctgatgc ttgtccacca cctgtgccat
     3121 acgggcaggc gtcggccagt tcggtacgcc ttcaaacatc agcgtggtcg cgccgcaggc
     3181 cagcgggccg tacagcaaat aactgtgtcc ggtcacccag cccacatcgg cggtgcacca
     3241 gtagatatcg cccggatgat aatcaaagac atatttaaag gtcagcgccg cgtacaccag
     3301 ataaccgccg gtagtgtgca gtacgccttt tggttttccg gtagaaccgg aggtatagag
     3361 aataaacagc ggatcttcgg cgttcatctc ttccgcctgg tgctgatcgc tggcttgctc
     3421 aacctggtcg tgccaccaca ggtcgcgccc ttcctgccag tcaattttcc cgccagtacg
     3481 cttcagtacc accacatgct ctacgctggt gacgttcggg ttttttagtg cgtcatcaac
     3541 gtttttcttc agcggaatac tacgcccggc gcgcacgcct tcgtcggaag tgatcaccag
     3601 tcgtgagttg gaatcgataa tgcgcccggc aaccgcttcc ggcgagaagc caccgaaaat
     3661 taccgaatgc accgcgccaa tacgggcgca ggccagcatc gcaaccgcgg cttccggcac
     3721 catcggcata taaatcgcca ccacatcacc ttttttaatg cccagcttga gcagggtatt
     3781 ggcgaagcgg cagacgtcgc ggtgcagctc tttatagctg atatgtttgc tctggctggc
     3841 gtcgtcgcct tcccagatga tggcggtacg atcgccgttt tcttgcagat ggcggtcaag
     3901 gcagtttgcc gccagattca gcgtgccgtc ctcgtaccat ttaatggaca cattaccggg
     3961 ggcaaaggag gtgtttttca ccttctggta cggtttgatc cagtcgagaa tttttccctg
     4021 ttcgccccag aaggtatcag gtgcgttaat agattgttga tacatcgcct cgtactgctg
     4081 agggtttatc aggcaacggt ctgcgatgtt ggcaggaatg gtgtgtttgt gaatttgact
     4141 catgcttttg ttctccttgt aggatgttaa taatatgtgg cataagcgtt aaatgtaggg
     4201 gtattggcag ttttgtttag tatttgggcg acagatcacg caaaagtaga attgtgcaaa
     4261 taaacggcag ggtaattttt gaaggtcagc aacaaaagtt gattaattct ttgaggaaca
     4321 tgcagttatg catgctgttg aaaaagagga agatactgac taactctaaa gtggtatttt
     4381 acatgcactt acaattgatt aaagacaaca ttcacagtgt ggttatttgt tacacttagg
     4441 ggcgagcaat gtcatgacag tgtaggtgcg gttactgtcg tgaaaaacaa taaaaaccgc
     4501 cattgcaaca atggcgcaat tcggatgaag cccctatgac aaggataaaa ataaacgcac
     4561 gccgtatctt cagcttattg attccttttt tctttttcac ttctgttcac gctgaacaaa
     4621 cggctgctcc cgcaaaacct gtaactgtgg aagcgaagaa tgaaaccttt gccccgcagc
     4681 atcccgatca atatctctcc tggaaagcca cctcggaaca gtcagagcgt gttgacgccc
     4741 tggcggaaga tccacggctg gtgatcctgt gggcggggta tcccttctcg cacgattaca
     4801 acaagccgcg tggacatgcc tttgctgtga ccgatgtgcg tgaaaccctg cgtaccggtg
     4861 cgccgaaaaa cgctgaagat ggtccgctac cgatggcgtg ctggagttgt aaaagcccgg
     4921 atgtggcgcg tctgatccag aaagacggcg aagatggcta cttccacggt aaatgggcgc
     4981 gcggcggccc ggaaatcgtc aacaacttag gttgtgccga ttgccataac accgcctctc
     5041 cagagttcgc caaaggcaaa ccggagttaa ccctttcccg tccgtatgcg gctcgcgcga
     5101 tggaagccat tggtaaacct tttgagaaag ccggacgttt cgaccagcaa tcgatggttt
     5161 gcggtcagtg ccatgtggag tattacttcg acggcaaaaa caaagcggtt aaattcccgt
     5221 gggatgacgg catgaaagtc gaaaatatgg agcagtatta cgacaaaatt gccttctctg
     5281 actggactaa ctccctgtcg aaaacgccaa tgctgaaagc gcagcacccg gaatatgaaa
     5341 cctggacagc gggcattcac ggtaaaaaca acgtgacctg tatcgactgc catatgccaa
     5401 aagtgcagaa cgccgaaggc aaactctaca ccgaccataa aattggtaat ccgtttgata
     5461 acttcgccca gacttgtgcg aactgccata cccaggacaa agctgccttg caaaaagtgg
     5521 tcgcggaacg taagcagtcg attaacgacc tgaaaatcaa ggttgaagat caactggttc
     5581 acgctcactt cgaagcgaaa gcggcgctgg acgcaggtgc gacggaagcc gaaatgaagc
     5641 cgattcagga cgatatccgt catgcccagt ggcgttggga tctggcgatc gcttcccacg
     5701 gcattcatat gcacgcaccg gaagaaggtc tgaggatgct cggtacggcg atggataaag
     5761 cggcggatgc acgcaccaaa ctggcgcgcc tgctggcgac taaaggcatc acccatgaaa
     5821 tccagatccc ggatatctcg accaaagaga aagcccagca ggccattggc ctgaacatgg
     5881 aacaaatcaa ggccgagaag caggacttca tcaaaacggt gatcccgcag tgggaagagc
     5941 aggcacgtaa aaacggtctg ttaagccaat aaccccgttc cgcctcgcaa ggggcggaaa
     6001 acacaatgga gtgaatatga gcgtattacg ttcgttatta actgccgggg tgctggcgtc
     6061 gggcctgttg tggagcctga acggaattac cgctacccct gcggcgcagg catctgatga
     6121 tcgctacgaa gttacccagc agcgtaaccc ggatgccgcc tgtctggact gtcataagcc
     6181 agataccgaa ggtatgcatg gcaaacatgc ttccgttatc aacccgaata acaaactgcc
     6241 ggtcacctgc accaactgtc acggccagcc atcaccgcaa caccgcgaag gggtgaaaga
     6301 tgtgatgcgc tttaacgagc cgatgtacaa ggttggagag cagaacagcg tctgtatgtc
     6361 ctgccatctg ccggaacagt tgcaaaaagc gttctggccg cacgatgtcc acgtaaccaa
     6421 agtggcgtgc gccagctgcc attccctgca tccacagcaa gatacgatgc agacgttaag
     6481 tgacaaagga cggattaaga tttgcgtcga ttgccacagc gatcagcgca ccaatccgaa
     6541 ctttaacccg gcgtctgttc cgttgcttaa ggagcagcca tgacctggtc tcgtcgccag
     6601 tttctcaccg gcgtcggcgt gctggcagcc gtcagcggca ccgcagggcg cgtggtggcg
     6661 aaaacgttga atatcaatgg ggtgcgttac ggcatggtgc atgacgagtc gttatgcatc
     6721 ggctgtaccg cctgtatgga tgcttgtcgg gaagtgaaca aggtgccgga aggcgtctcg
     6781 cgcctgacga ttattcgtag cgagccgcag ggcgaatttc ctgatgtgaa atatcgcttc
     6841 ttccgtaagt cttgccagca ctgcgatcat gcgccgtgcg ttgacgtctg cccgaccggt
     6901 gcatcttttc gcgatgctgc cagtgggatt gtcgatgtta atccggatct ctgcgtcggt
     6961 tgtcagtact gcatcgccgc ctgtccgtac cgcgtgcgct ttattcatcc ggtcacgaag
     7021 acagcggaca aatgcgattt ctgccgtaag accaatttgc aggccggtaa gcttcccgcg
     7081 tgcgttgagg cttgcccgac gaaggcgctg acgtttggca atctggacga tcccaacagt
     7141 gagatttcgc aactgctgcg ccagaagccc acttaccgct acaagctggc gctgggaacc
     7201 aaaccgaagc tgtaccgcgt accgtttaaa tacggggagg tgagccaatg acgcagactt
     7261 ccgcatttca ttttgaatcg ctggtgtggg actggccgat tgccatctac ctgtttttga
     7321 ttggtatttc tgccggtctg gtgacgctgg ccgtgctgtt acgtcgcttc tacccgcagg
     7381 cgggcggtgc agacagcacg ttgctgcgca ccacgctgat tgtcggacct ggcgcggtga
     7441 tcctcggtct gttgagcctc gtcttccacc tgacaagacc gtggaccttc tggaagctga
     7501 tgttccacta cagttttact tcggtgatgt cgatgggggt gatgctgttt cagctttaca
     7561 tggtggtgct ggtgctgtgg ctggcgaaaa tctttgaaca tgatttgctt gccctgcaac
     7621 aacgctggtt gccgaagctg gggatcgtgc aaaaggttct gagcctgctg acgcccgttc
     7681 atcgcggact ggaaacattg atgctggtgc tggcggtgtt gttgggggct tataccggtt
     7741 ttctgctgtc ggcgctgaaa tcgtatccgt tccttaataa cccgatcctg ccggtgctgt
     7801 tcctcttctc cggcatctcg tccggtgcgg cggtggcact gatcgccatg gcgatacgcc
     7861 aacgcagtaa cccgcattcc acggaagcgc agtttgtaca ccgtatggaa atccccgtgg
     7921 tatggggtga aatcttcctg ctggcggcgt tttttgtcgg tctggcgctg ggcgatgacg
     7981 gtaaagtgcg tgcgctggtg gcggcattgg gcggcggttt ctggacgtgg tggttctggc
     8041 ttggtgtcgc cgggctgggg ctgattgtgc caatgttgtt caaaccgtgg gtcaatcgca
     8101 gttccggcat tcctgccgtg ctggcggcgt gtggggccag tctggtcggc gtgttgatgt
     8161 tgcgattttt cattctctac gccgggcagt taacggtggc gtaagccaga acagaggtgg
     8221 tttctggacg tattccttcc tgaagtcggt tttctggcgc tgttgttaag tctcggggtc
     8281 aacgtgttga ccccgttgac ggcctttgcg ggagtgcggt tgcgctggcc tgccatgatg
     8341 cgactcactt gcatcggcat tctggcacag ttcgcgctcc tgctgctcgc ctttggcgta
     8401 ctgacgtatt gttttctcat cagcgatttc tcggtcattt atgtcgccca acatagctac
     8461 agcttgctgt cgtggaaact caaactggcg gcggtgtggg gcggtcatga aggttcgctg
     8521 ctgctttggg tgctgctgct ttccgcctgg agcgcgctgt ttgcctggca atatcggcag
     8581 caaaccgatc cgctatttcc gctgacgcta gccgttttat ctctcatgct cgccgcactg
     8641 ctactgtttg tagtgctgtg gtccgatccc ttcgtgcgga tatttccacc agcaatcgaa
     8701 ggccgcgatc tcaatccgat gctgcaacat cccggtctta tctttcatcc accgctgctt
     8761 taccttggct atggcggttt gatggtagcg gcgagcgtgg cgctggcgag tctactgcgc
     8821 ggcgagtttg atggtgcctg cgcccgaatt tgctggcgct gggcgttacc tggctggagt
     8881 gcattaactg cggggatcat cctcggttcc tggtgggcct actgcgaact cggctggggc
     8941 ggctggtggt tctgggatcc ggtggaaaac gcctctttat taccctggct ttctgccact
     9001 gcgctgctgc acagtttatc cctgacacgc cagcggggga tttttcgcca ctggtcgctg
     9061 ttgttggcga tagttactct gatgctgtcg ctgctgggca ccttaattgt ccgttctggc
     9121 attctggttt cggttcatgc gttcgctctg gataacgttc gcgctgtgcc gttgttcagc
     9181 ctgtttgcac tgattagcct tgcgtctctg gctctgtatg gctggcgagc gcgggacggt
     9241 ggcccggcgg tgcgtttttc ggggttatcg cgggaaatgt taatcctcgc tacgctgttg
     9301 ctgttttgcg cagtgctact gatcgtgctg gtgggaacgc tttatccgat gatttacggc
     9361 ctgctgggct ggggacgtct ctccgttggc gcaccgtatt ttaaccgcgc gacgttaccg
     9421 tttggtctgt tgatgttggt ggtgattgtg ctggcgacgt ttgtctctgg caaacgcgcg
     9481 cagcttccgg cgctgctggc gcatgcgggc atactgttat ttgccgcagg gatcgttgtc
     9541 tccagcgtca gccgtcagga gatcagcctg aatttacagc cgggtcagca ggtgacgctg
     9601 gcaggataca ccttccgttt tgagcgcctc gatctgcaag ccaaaggcaa ttacaccagc
     9661 gaaaaagcga tagtggcgct gtttgaccat cagcaacgca ttggtgaatt aacgccggag
     9721 cggcgttttt acgaagctcg tcgtcagcaa atgatggaac cgtcaattcg ctggaacggc
     9781 atccatgact ggtatgcggt catgggggag aaaactggag cggatcgtta cgcttttcgc
     9841 ttgtatgtac aaagcggtgt gcgctggatc tgggggggag gattgttgat gattgcgggc
     9901 gcattgttaa gcggatggcg ggggaagaag cgcgatgtat aaagggcttc tcacgctact
     9961 gctgttattt acctgttttg cgcgcgctca ggttgtagac acctggcaat tcgccaatcc
    10021 gcaacaacag caacaggcgt taaatattgc cagccagtta cgttgtccgc agtgccagaa
    10081 tcaaaactta ctggaatcca acgcgccggt ggcagtcagt atgcgccatc aggtttacag
    10141 catggtggcg gaggggaaaa gtgaagtcga aatcattggc tggatgaccg aacgctacgg
    10201 agattttgtt cgctataacc caccgttaac gggtcagacg ctggtgttgt gggcgctgcc
    10261 agtggtgttg ttactgctga tggcactgat cctctggcga gtgagggcga agcgatgaaa
    10321 cagcctcaaa taccggtgaa aatgctgaca acactcacga tattgatggt attcctctgt
    10381 atcggtagtt atctgttaag cccaaaatgg caggcggtac gtgcggagta tcagcgtcag
    10441 cgcgatccgc tacatcagtt tgccagccag caaaacccgg aagcgcagct tcaggcattg
    10501 caggataaaa tccgcgctaa tccacaaaac agcgaacagt gggcgttact gggcgagtac
    10561 tatctgtggc aaaacgatta cagcaattcg ctgctggcgt accgtcaggc gttgcaacta
    10621 cgtggtgaga acgctgaact gtatgcggcg ctggcgacgg tgctttatta ccaggccagc
    10681 cagcatatga ccgcccagac tcgcgcaatg atcgacaaag ccctcgcgct ggactctaac
    10741 gaaatcaccg ctctgatgct gctggcttcc gatgcgttta tgcaggcgaa ttacgcgcaa
    10801 gccatcgaac tatggcaaaa agtgatggat ctcaactcac cccggatcaa ccggacacag
    10861 ctggtcgagt cgattaatat ggcgaaattg ttgcagcgga gatcggatta atcatctggt
    10921 gatgaagatt atgttattga gctgcataaa aaataatcga atgaacatat gccaaaaata
    10981 atcactaatc agtattattg cagattaaac aaataaaaat ctttccataa caaatggtta
    11041 ttcattaatc ctgccataaa ctcgcgttat tatgcattaa tgcagcgaaa agctctgttg
    11101 ttaaagggtt gcgcaacata ccgcgcaaat gatactgatc ataagcgtta aaaaaatcta
    11161 caaaccaacg caacacaatt catgccctgg cagtatgtca cgttctcgcg tttctgaacg
    11221 gggaacggcg ctccattgag gaagtcattc atatgaaaaa tataaaattc agcctggcct
    11281 ggcagattct gtttgctatg gtgctgggca ttctcctggg aagctacctg cactaccata
    11341 gcgacagccg cgactggctg gtcgtcaatt tgctctctcc ggcgggtgat atcttcatcc
    11401 atctgatcaa aatgattgtt gtgccgattg tgatctccac gctggtggtg ggtatcgcgg
    11461 gtgttggtga tgccaaacag ctcgggcgta ttggcgcgaa aaccattatc tacttcgagg
    11521 tgatcaccac cgttgccatc attttgggga tcactctggc gaacgtcttc cagcccggtg
    11581 ccggggtgga tatgtcgcag ctggcgaccg tcgatatctc gaaatatcag agcactacgg
    11641 aagcggtaca aagcagttcc cacggcatta tgggcacgat tttgtcgctg gtgccgacga
    11701 atattgtggc gtcgatggcg aaaggcgaaa tgttgccgat catcttcttc tcggtgctgt
    11761 ttggtctggg gctttcctcc ctgcccgcga cgcatcgtga accgctggtg accgtgttcc
    11821 gctccatctc tgaaaccatg tttaaagtga ctcacatggt gatgcgttat gcgccggtgg
    11881 gtgtgtttgc gctgattgca gtgacggtgg ctaactttgg tttctcttct ctgtggccac
    11941 tggcgaaact ggtgctgctg gtgcatttcg ccattctgtt cttcgcgctg gtagtgctgg
    12001 gaattgtggc gcgcctatgc gggttaagcg tctggatcct gattcgtatt ctgaaagatg
    12061 agctgattct ggcgtactcc actgccagct ctgaaagcgt actgccgcga attattgaga
    12121 agatggaagc ctacggagca ccggcgtcga tcaccagctt cgtggtgccg accggttact
    12181 cttttaacct tgatggttcg acgctgtatc aaagtattgc cgctattttc atcgctcagc
    12241 tgtatggcat tgacctgtcc atctggcagg aaatcattct ggtgctgacg ctgatggtga
    12301 cctcgaaagg gattgctggc gtgccgggcg tgtcgtttgt ggtgttgctg gcaacgctgg
    12361 gtagcgtagg tattccgctg gaaggtctgg cgtttattgc tggtgttgac cgtatcctcg
    12421 acatggcgcg tactgcactg aacgtggtgg gtaatgcgct ggcggtgctg gtgattgcca
    12481 agtgggaaca caaatttgac cgcaaaaagg cgctggctta tgagcgtgaa gtgctgggca
    12541 aatttgataa aactgcggat caataattga agattgccgg ggatatcccc cggcaatgtg
    12601 tggatgcctg atgcgacgct ggtgcgtctt atcaggccta cgtcagacag cgctagcgtg
    12661 attttgtagg tctgataagg cgtttacgct gcatccgaca tcaacgcctg atgcgacgct
    12721 taacgcgtct tatcaggcct accttaaccg ttagttaatt tttcaaactc ttcacacccg
    12781 gtatcaaacc cttccataca gctcaggttc aaccagtgca gcgccttttg cttgttcttc
    12841 tcgataaaac cttcttcacc gtttaagaac atcattcccg cccagtactc ggaataaccg
    12901 gttcgggaaa ttgcagagct gcgtttgaaa taccaggttg ccttgtcatc gtcggcctta
    12961 atgcccacgc cgttggcgta aatcaaacca agcagcattt gggcatcgac ggcagagtcg
    13021 ttctccagat cttccgaggc gttttctaac agcgaaatcg cctttggata atccggttta
    13081 cccgcttgag tatttaccag aatatgcgcc agcgttactt caccttcttt actgcccgct
    13141 tgcgccgctt tctctgcaag aacttttgct tgtggatagt ccagactgac cggattggtg
    13201 attttgatct gcgccaacag cgcgcaggca tcagcatcac cgctgtcggc ggctttctgt
    13261 gcccaatatt cggctttgct caaatcgccg gagctaaacc agctgtcggc aagaaaatat
    13321 tgtgcgcgtc ggtccccggc ctctgctgcc tttaaatact ggctgccagg ctcggagtcg
    13381 ttggcgtggg caaagaatgt caaaaacaac atcaatgcga taattttttt cattttgata
    13441 atacgattgg gtagggagta accagtataa agagttcgaa tggataaaaa aacagcctcc
    13501 gaaaggaggc tgtagaaagg actgtattac gccagtgccg cttcgcgcag gcgagttttc
    13561 aacttgttgt actcgtcaat cacgtactgc tcggcggcgc gctgatcggc gatcggctcg
    13621 acgcgcacgg cgcagtattt gtattccggc gttttcgtaa tcgggcttaa gttttcggta
    13681 accagctcgt tacaggcacc aatccaccac tggtaggtca tgtaaatcgc ccctttgttc
    13741 ggacgatcgc tgacctgcgc acgggtgata attttgcctt tacgcgagtg cacccaaacc
    13801 aatgcctcat cttcaatact cagacgtttg gcgtcttcgg tattgatttg tgcgtagcca
    13861 ggttcatcag ccagcgccgc cagtgccgca cagttaccgg tcatcgaacg gcaagagtag
    13921 tgaccaactt cacgcaccgt tgacagtacc atcgggtact cgtcggtgag tttgtcgatt
    13981 ggcgctaccc agtcgcaggt gaagaactgc gccagaccgt tcggggtgtc aaacttctct
    14041 ttaaacagat aagaagtccc ctgatcggca tctgaagtat cgcggcaagg ccactgaatg
    14101 aagcccagtt cgcccatttt ctcgtaagtc gcaccgtaga aatccgggca cagatgacgc
    14161 aactcatccc agatctcctg ggtgttgttg tagtgcatcg gataacccat acgggtggcg
    14221 atttcactga tgatttgcca gtccgttttc agatcccatt tcggttcaac tgctttgaag
    14281 aaacgctgga agccacggtc agccgcagta aacacgcctt catgctcgcc ccacgacgtt
    14341 gacggtaaaa taacatccgc cgccgacgcg gttttggtca taaagatgtc ctgaacgata
    14401 accagttcca gatcttcaaa ggctttacgt actgccgaca gctccgcgtc agtttgtagc
    14461 ggatcttcgc ccataatgta cgcggcacgc acttcgccat gcgctgcgcg gtgcggcagc
    14521 tcgctgatgc gataaccggt atgagcaggc aggctttcca cgccccaggc tttggcgaat
    14581 ttctcgcggt tagccggatc ttttacgtac tggtatcccg gatacgtatc cggcagcgcg
    14641 cccatatcgc aggcaccctg cacgttgttc tgaccacgaa ccgggttaac acccgcatgt
    14701 ggcttaccga ggttaccggt cagcatcgcg aggctggtca gagaacgcac ggtttccacg
    14761 ccctggtaga actgggttac acccatgccc cacaggatgg cggcgctttt cgcctgggca
    14821 tacatccgtg ccgcctgacg aatctcactg gcgctgacgc cggtgatatc ttcaaccgac
    14881 tccggcgtgt agccttcaac gattttacga tactcttcaa agccttctgt acgggaagcg
    14941 acgaatgctt tgtcgtacag attttcttca ataatgacat ggcccatcgc attcaacagc
    15001 gcgatgttcg agccgttttt cagtgcaatg tgcatgtcag caatgcgcgc ggtttcaatt
    15061 ttgcgcggat cgcagacgat aattttcgcc ccgttacgtt tagcgttaat tacgtgattc
    15121 gccacgattg ggtgggaatc cgccgggttg tacccgaaaa cgaacactaa atcggtatta
    15181 tcaatttcgt taatagcatt gctcattgcg ccattaccga ccgattggtg cagacctgca
    15241 accgatgggc cgtgtcagac acgagcgcag cagtcaacgt tattggtacc aataacggcg
    15301 cgcgcaaatt tttgcattac atagttggtt tcgttacccg taccacgcga ggagccggtc
    15361 gtctggatgg catccggacc gtacttctct ttgatggcgc tcaggcgctc ggcaacgtaa
    15421 ttcagtgcct catcccagga aacaggttcg agtttgccgc cacgctggcg acggatcatg
    15481 ggggttttca ggcgcggggt caggatctgg gtatcgttaa tgaagtccca gccataataa
    15541 cccttcagac acagggtacc ctggttggtt ttcccctgcg ctgcctccgc ccggacgatt
    15601 ttgccgttat cgacgaccag gttgattttg caacctgatg cgcaataggg gcaaaccgtg
    15661 acgacttttt tcatcggtct cgctccagtt aatcaaatca cgcatacgcg ctctcgacta
    15721 cagtatgcat cttttatgcc acattttatt tggggtcatt ccctgatatt acgggcacta
    15781 tttattcaaa actctgacga aaaacaggct gtcgtcagtt ttgacgtgac gaaacgaaat
    15841 accgcgtgac agccatcaca cggcagacat tttatttttt ctcgacgacg ggagccgcct
    15901 gatacccgct gcccagcgat ttcatcaact gaatgctttg gatcacccgg cggctgtcca
    15961 gcatcagtaa tgacatctct tcggcaagca ccggcaaccg ggcttcggta gcctgtaagc
    16021 ggctggttaa gccgcgctga taggcggcct cggcagcgcg ctgggtgaag cgcgttgctt
    16081 ccacgcgttc agcctgcatt tctcgctcgt cgttgagcgt ttgcagacgc gtgccgttga
    16141 cggcaacgtc acgcaccgcg ttcagtactg actggttgta acgttcaatc atcatgttgc
    16201 tggcggcgcg agtgccttcg agattggcat tcaaccgtcc accgtcaaac agcggcaatt
    16261 tcagacccgg gatgaagttg aactggcgac tggttttttt gaataaggta tccaggtgga
    16321 tggagtccag accgaaaaac gctttgatat caaagcttgg gtagaacagc gcccgcgcgg
    16381 aatccacctg atctaatgac gcctgaacat accagcgcat ggcttgcaga tccgggcgtc
    16441 tggcgagcaa ctcataagag agtgtcgccg gaataccggt ctggactcgt ggtaatgcca
    16501 ctggtttgat ctccggcata tcgctggctc ccgcgccaat caatgcacgc agagattctc
    16561 gcgtttcggt gatttgccct ttaacggcag caatttgttt atcgaccgcc agaatctgtg
    16621 cccgcgcgcc gtggaaaggc acttgcgctt ccagaccgtg cgccacttta ctttggtgcg
    16681 ctttcaccgc gtaatcaatc acatcgcgag tttgttctaa cagatcgagc atctgatagc
    16741 tggcctgcat actgtaataa agctgcgcta cgcccgtggt cagcgatagc tctactgctg
    16801 cggtttctgc cagcgcggca ttatgcgcgc caatggcggc ggtaaccgct gagcgatgca
    16861 caccccacaa atcaagatcc agtccggcaa acaaaccgac tgtggcttcc gtatagtacg
    16921 gcccgtccat acccagtgct ggcgcatcca tcgcataagg gcttaaaaag ccgttcgccg
    16981 agacgcgttg gcggttgagc atccctaacg ctgcgacctg taattgtgaa ccggcatcta
    17041 acaaatcggc ctgggactgc gctttttctt cccgcagttt cgcttcggcg agggtgtgtg
    17101 aaccacttag cgtccgttgg atcagcgcat ccagctgcgg gtcattgagt tgtttccacc
    17161 actgcgcctg cggccagccg gagctggcaa gatgaatatc atcggccagt ttgatttgtt
    17221 ccggtttgag ctgttgatga ggcgcagaat ccttgcgtac cagggcacag ccggaaatca
    17281 gcgtcgtgct gccgagaatg ctgcacaaca gcagacgtga aagttgacga ttgatcattg
    17341 cgtggctcct tgtgcctgtt ccgtggcggg cgttaatgcc ggggctgtcc agtctggcaa
    17401 gcgggcgaaa agctggcaga cgtgttgctc ctgttttaat aaggtgggca ggatggcctg
    17461 cgaggcgggt gtctcttcaa gcgttatttg aggagaacgg ctgagtgcgg ttgccagacc
    17521 ggcagcgtat ttttccaggg cgtcggcaaa ctgacctgct ttgtccggct gtagtgcgtt
    17581 atccagcgcc tgcgccgagt tccaggtggc atcccaggcg tgaagaagat cgcggccctg
    17641 atgaataacc gtttgcgaac gttcaatcag taaggcgcgt tcttcgctgt ccagttgacg
    17701 ctccagcgcc acgcgttggc acatctcttc acaggcatta aacgccgcat gcagacctat
    17761 acgaatttgc agataagtgc gcagagccgt gacttcctgc tggcgtggga tccgcattac
    17821 tttacttagc atacccagcg cgccagccag tttttgcggc agcgtgcgcg cttcactttc
    17881 aggccagaca aaggtgtaaa tcaccgcaga caccacggta ccaatgatga tacccagggc
    17941 gcgatcgcga atttccacca ggtcgtacac cggaccaaaa acgttttcga gcgtggcgag
    18001 cgcgaaggtg accaccatct gtgtgccgat ataagaagag cgttcagagc tggtggcaat
    18061 ccatgcgccc aacaggaaaa tcggtgccag cacaaacagc aattcgacaa tattgtccag
    18121 ccagggcatg accagtagcg tgaataacag cgccagaatc gcgccgcaaa aggccccgcc
    18181 aaaacgcagc accatcttct ggtacgatga accgacattc ggattagcga cgatcacgca
    18241 ggtcagcata caggtgtgaa tgccttccca gtccacgccg ctgtagaagg tgtaacagat
    18301 caaacaggcg agcagcgttt ttaccgcgta gcgcatatag tctggattgg taaaagcatc
    18361 agcgaccatt gatggcggtt tggcggcggg cgttggcggc gtattcgggt ccatctgacc
    18421 cagttgtaac aacgtctggc agatattctc caggttacat tcccgtgccg ccatcgcttc
    18481 actttcactg atccgccagt cgctttgcca gcactgacct tcggtaatgg catgctgcag
    18541 cttgttgatt tctgaagcta atttttgtcg gaattcaata attgcctgag aatcagcaaa
    18601 agaggtggga tcgtagcgat tcagcgtcga gtaaatgtag gttaccgttg ccacgcagct
    18661 ttgccaccat gcgctttgag ttcgccagtt ggcatcgtcc gcgaggcaaa agacattgag
    18721 tttttgcagc gccagcgcct ctctttcaat ccgcgtttcg ggtagcggtg cgaggctgtc
    18781 cgtcaggtgg ctaatggcat catcaagccg atcattaagc gcttgatgca tttgcgaaat
    18841 ggcacgactg ggaaaccaca gtacgccgat taacgtcatt aataacgtcg ggtagaggcc
    18901 aacaacgata caccacagcg ttaagcgcac gaccacttcc ggatagtcga gcatggcggg
    18961 gaaggtttgc ccataaatgg cgacaatggc gacagcgaaa aagaccagtc ccaaccgatg
    19021 ggtgcgcatc aaaaacatgc agcccatcag gatcggcccg gcgatgatca agcggatcaa
    19081 cggttcgccg tatgaccatt tgtagatcaa aaacaggctg ccgatctcca gcaccgtggc
    19141 aaccacaaac aagatcgcga caaatttggt gtaaaacgcg ttcgactgaa taccgtaaaa
    19201 cagcactgcc agcgataacg ccacaaaagg gatctcaaaa gtcatcgaga tcagaatcac
    19261 cagcaggcag cctacccaga gttgcacggt ctgcggcacg cgacctggtc gccgctcgct
    19321 taactcttca tgaaagaacg ccagcagcct gaccaccggt aatggcaggg agttgagcgc
    19381 gctcattatt gcggctcaag attagcgact gccgaagcgc cgatgcggaa catttccggg
    19441 tcaggtttat caaccatgat tttgaccgga aaacgctggg caacacggac ccagttaata
    19501 gaacgagaaa ctttcggcag gccgcccagc accaggccgc cgtcatccgg tagcacgccg
    19561 tagccaattg aatccacttt accctcgaaa gttttgccgc tgtcactcat caggcgaatc
    19621 gttgcaggtg tacctgagcg aatatttttc agatcggttt cgcggaagtt ggcgatcacg
    19681 taccagtgac gagtgtcgat tagggtaaaa atagggcgca tggcagaagc aaattgcccg
    19741 acggaggttt tgagggaaat gacccggcca tcaaacggtg cgcgaacggt cgccatttcc
    19801 agatgcagtt tggtcagggc aatatccgct tcgaccgccg cacgctgggc aactaatgca
    19861 tccacgccgc tgacggcgct ggcggctgac tgcgcctgca acaatacagc attaagatcg
    19921 gcttctgcag cgcgctgcgc ggtcctcgca cggtcaacgt cttccgctga gacaaaacct
    19981 tctttcagta atggctcggt gcggcgtaat gtatctgtgg cctgtttagc ggcggcacgg
    20041 gctttttcta ccgtggcgtt aaccgagtcg gcaccaaact gttgcgcgtc aacgctacgc
    20101 tgggtgagca taatttgctt atccagtgcc gcgagggagg cttcagattt cgccagattg
    20161 gcttcgtacg ggcgcgggtc aatgcggaac agcaaatcgc cctgtttgac tgcctggttg
    20221 tcggtgaccg ccagttctac aatgcggccg ctgacttccg gcaccacatc aatggtatct
    20281 gctgacacgt aagcgtcatt agttgatggc gcactgtcta cgcgccagat aacgaaaaca
    20341 agggcaacca gcgccaacgc aaccactaac agagcaggga atttactgcg aggagctttt
    20401 ttcggcgtac tttccatgag cgcgtctcaa atagattaga aaaatgccag ccagaacagc
    20461 atggcgtaga gagcaaaaag ggcggtataa ataattccga caaatgccag cttgatattt
    20521 gcccgctgaa taactcgcct cgtgatgagc gtcaatataa ggctggcaat ggcgcagaaa
    20581 aaccagctgg gataataagc gccgatcacc gggattgccg gagataatga gcatcccgaa
    20641 accatgaaga cgggaattat ccaggctgtt tttttgagtt gcatcgccat gcgagagaga
    20701 actgttggca tattaaatac atcctgtctg ctgcgaacaa tatctgtagt gaatatcaat
    20761 atccattgaa tgcgtcagta tggctatttt cttgatgaat aaaaatagct t
//
LOCUS       DBFIFM010000068        20677 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.068, whole genome shotgun sequence.
ACCESSION   DBFIFM010000068 DBFIFM010000000
VERSION     DBFIFM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.068"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(379..810)
                     /locus_tag="N7C74_002185"
     CDS             complement(379..810)
                     /locus_tag="N7C74_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HGU2432050.1"
                     /translation="MVLRSIWVEEQYMGVKKIVILAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(878..2899)
                     /locus_tag="N7C74_002186"
                     /pseudo
     CDS             complement(878..2899)
                     /locus_tag="N7C74_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3033..3158)
                     /locus_tag="N7C74_002187"
     CDS             complement(3033..3158)
                     /locus_tag="N7C74_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HGU2432051.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK"
     gene            complement(3100..3249)
                     /locus_tag="N7C74_002188"
                     /pseudo
     CDS             complement(3100..3249)
                     /locus_tag="N7C74_002188"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            complement(3423..4184)
                     /locus_tag="N7C74_002189"
     CDS             complement(3423..4184)
                     /locus_tag="N7C74_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HGU2432052.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRNQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFCVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWYARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAAKE
                     AGHAVREMERWMVPNKLREVRDAATITRIARKQPE"
     gene            complement(4181..4615)
                     /gene="psiB"
                     /locus_tag="N7C74_002190"
     CDS             complement(4181..4615)
                     /gene="psiB"
                     /locus_tag="N7C74_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HGU2432053.1"
                     /translation="MKTELTLNVLHTMNAQEYEDIRAAGSDARRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDTQGYSVNDIIHILMAEGGQV"
     gene            complement(4670..6628)
                     /locus_tag="N7C74_002191"
     CDS             complement(4670..6628)
                     /locus_tag="N7C74_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HGU2432054.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERDIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADTFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGFCREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADNDYRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQSD
                     KSLALLAWTLCLNVFGSGAYSNPARIRLECEHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPDGWARDMTTFLSLSQEVLLSLLSFCTACSIHGVQTREYGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPRPQTDATERTDNLADAA"
     gene            complement(6694..6927)
                     /locus_tag="N7C74_002192"
     CDS             complement(6694..6927)
                     /locus_tag="N7C74_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HGU2432055.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIENDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGIIK"
     gene            complement(6984..7436)
                     /locus_tag="N7C74_002193"
     CDS             complement(6984..7436)
                     /locus_tag="N7C74_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU2432056.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLVRAAGAQTQPEEGQQFSGQPQPQPPEGDDYGFSDDIPF"
     gene            complement(7462..7668)
                     /locus_tag="N7C74_002194"
     CDS             complement(7462..7668)
                     /locus_tag="N7C74_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU2432057.1"
                     /translation="MHIPQNFLPKSRQKVLRSAPGQSFPAVAVMPLAAKVLKKVMVNT
                     GGNKRQVPAKRQNRGSRNSKDDGG"
     gene            7738..8181
                     /locus_tag="N7C74_002195"
     CDS             7738..8181
                     /locus_tag="N7C74_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432058.1"
                     /translation="MAPDTTTGRHPPQRREARFAKRRCPLHPGGLLPGCRERRPRRDR
                     GGATGTVAASPGFASGMEARQGGDSSAGSVHDSPPRQGDARKRHKQENNKTDSNQSSR
                     KKKNSSSSSSSSSSMNNNIISNRQMTGLKRQKPTAECPRNVRGGA"
     gene            complement(8267..8830)
                     /locus_tag="N7C74_002196"
     CDS             complement(8267..8830)
                     /locus_tag="N7C74_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HGU2432059.1"
                     /translation="MHSQLKERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRVIINPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(8877..10238)
                     /locus_tag="N7C74_002197"
     CDS             complement(8877..10238)
                     /locus_tag="N7C74_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HGU2432060.1"
                     /translation="MTSAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERGRSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLNYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(10290..10520)
                     /locus_tag="N7C74_002198"
     CDS             complement(10290..10520)
                     /locus_tag="N7C74_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432061.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            11037..11546
                     /locus_tag="N7C74_002199"
     CDS             11037..11546
                     /locus_tag="N7C74_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432062.1"
                     /translation="MSRDPPQRREARFAKRRCPLHPGGLLPGCRERRPRRDRGCATGT
                     VAASQGFASGMEARQGGDSFAGSVHDSPPRQGDARKSHKQERNGKKRRKRKRKKKKNS
                     NRHGNHRKITSEKNIPDTSQTGRVTDDASAWPASLPAGVSLPGVFARADRRPPPPALY
                     HLFLLPCKM"
     gene            complement(11516..11707)
                     /locus_tag="N7C74_002200"
     CDS             complement(11516..11707)
                     /locus_tag="N7C74_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432063.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(11704..12126)
                     /locus_tag="N7C74_002201"
     CDS             complement(11704..12126)
                     /locus_tag="N7C74_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HGU2432064.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEYGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(12173..12598)
                     /locus_tag="N7C74_002202"
     CDS             complement(12173..12598)
                     /locus_tag="N7C74_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU2432065.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADACGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(13016..13792)
                     /locus_tag="N7C74_002203"
     CDS             complement(13016..13792)
                     /locus_tag="N7C74_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432066.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLVNRHGGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDTRRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARNEIRSMADALAKIRK"
     gene            complement(13838..14392)
                     /locus_tag="N7C74_002204"
     CDS             complement(13838..14392)
                     /locus_tag="N7C74_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HGU2432067.1"
                     /translation="MCLLNAWHARRCIAPIACSWRRIPKFWNWMRALKIEKETRMYGT
                     CETLCRELAAKYPGYTPLMLVIWSPEEIQALADGMDISLSDPEIRTVLARLEDIPEDQ
                     RIESGISSAAAMEIISNVSENRQVTVPAELLASLIQTAEQALWKREWAARDYGLAVPE
                     CVTRRQAVVNQARILLKNNTHENG"
     gene            complement(14286..14507)
                     /locus_tag="N7C74_002205"
     CDS             complement(14286..14507)
                     /locus_tag="N7C74_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432068.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LARQTLYRANRLFMEAYTEILELDACFKD"
     gene            complement(14508..15191)
                     /locus_tag="N7C74_002206"
     CDS             complement(14508..15191)
                     /locus_tag="N7C74_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HGU2432069.1"
                     /translation="MSRFIQGDCVRVMATIPGNGVDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(15268..15573)
                     /locus_tag="N7C74_002207"
     CDS             complement(15268..15573)
                     /locus_tag="N7C74_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432070.1"
                     /translation="MTETGGQPPVSFPVKDVAGLLFLLRRLTRRGRNAACGQCPASGR
                     ARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIFSPSKRRRLRYSTVGLTRYRTR"
     gene            complement(15577..16479)
                     /locus_tag="N7C74_002208"
     CDS             complement(15577..16479)
                     /locus_tag="N7C74_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HGU2432071.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTHLQDGVLLDEQNCLMLHELWLQSG
                     TGRRRWEELPDDARESITALFTPKRGDWCDIWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLSTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYEVNISSQGDNFIQVDF
                     DTPWCQPESDVIAVLSRRFSCMLEHWYAGQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTAPEWIVDKVAHYGG"
     gene            complement(17174..18145)
                     /locus_tag="N7C74_002209"
     CDS             complement(17174..18145)
                     /locus_tag="N7C74_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HGU2432072.1"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            complement(18145..19311)
                     /gene="sopA"
                     /locus_tag="N7C74_002210"
     CDS             complement(18145..19311)
                     /gene="sopA"
                     /locus_tag="N7C74_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HGU2432073.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            complement(19899..20654)
                     /gene="repE"
                     /locus_tag="N7C74_002211"
     CDS             complement(19899..20654)
                     /gene="repE"
                     /locus_tag="N7C74_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepE"
                     /protein_id="HGU2432074.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVD
                     QIRKSDGTLQEHDGICEIHVVKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEED
                     AGDEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNP
                     YAMRLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINS
                     RTPMRLSYIEKKKGRQTTHIVFSFRDITSMTTG"
BASE COUNT         4720 a         6059 c         5207 g         4691 t
ORIGIN      
        1 tcaatcgcaa agcataacct tacctttctg cttacttatg cagttcaaag agtaccaact
       61 ctgcaatttt cgttgccaca gatgcaatat cgttactttc cagtcaccat gccgctgaaa
      121 gcccacacca tgcgataatc cacatcaaga gccgctttct gtccagaccc gctgttgtga
      181 ggtgtgaggt gtgaggtgtg aggtgtgagg tgtgaggtag cctgagttta acggacaggc
      241 cgatgaagga cgccgttacc gggataatgt aagaatcttt ttttgagtat gtcaacataa
      301 cctgaggcaa agtacctcag gttccagtaa tcagccgacc tgacagtctt ctgttagccc
      361 tacagatatg tcgtgtagct aaccgcgcat gtcaaagccg gatttttgaa aatctctata
      421 aacttccgga ttgttgaagg ccgggaattt agctttatgg gctgctgact ttatcgcttc
      481 gcaataggct ttatcgccat tactggttga tatttttaac gccgtgccat cccgggagaa
      541 ttccatatgc aacctgcatt ttttcctttt ccatttttgc ggatcatcaa gtttagcatt
      601 aattgcatcc ctgattcccc gtgcctgctc cccccattca tcctgatcat cccaacgtcc
      661 tgaactgcaa ctacccaggg ctgtagtttt atggcaattt gaaggatgta atggcgcaca
      721 tcccgccaca aagctgacta aaatagccag tataacgatt ttctttaccc ccatatattg
      781 ctcctcaacc catatagatc tcagtaccat agctctgtca gagcaaagaa aatcttaact
      841 aatacaagtt aagattttta atatccactc ctcatcccta cattccaggt gatggtggcg
      901 tccactcatt atcctgaact ggagcataag atgcgccatg ccccccggat atgcttttct
      961 gttgcctgat atattcgtgg tagtactgtt cactacgctg acgatgtaaa tccacatccg
     1021 ggcggctgat tacaaagtgt ccatttccgg ttattccagc tgcattatgc caggccacca
     1081 tttccgcaaa tacatcatta aactgttgat atgagccttc cggttcaaaa cccattcctg
     1141 gctcacgatc aaacaataca ggagcaatca gattcagctt atccaccagt tcatttctgt
     1201 cctgatgatg gtagaacacc acacggtccc cataatccac acagactaac tcgccattcc
     1261 tccgatcacg aaaacgcaca ctgccatctt tctgcagacg ggcttcgaga acgccagtgt
     1321 cttcataaag tggagttcct cccggttcac agagaatcac gcagcggtcg gcattcgttg
     1381 ttcgtcgctt gtctttgcga cgatcacgat aatcccagcc acgtaactgt gataatgcag
     1441 ctctgtctcc ctgtaccgcc tgttgctcca cccactgccg gtaggacaaa ggataccact
     1501 taccctcctc aatcagtgac aaccgctctt ccttcacact ctccttcagc ctgatcagcg
     1561 cctgcatacg ctgcacttcc gcaatatggt aatgcagctt acgcaattgt ggatcccgga
     1621 actgcacacg gatatacgcc ttgcgccgac ggcaggcagc atgaatttcc cgtaaccgtt
     1681 ctccgtaacg taagtcgggt ttacgccagt gctccttcca tgcaagataa cgcgcgcgca
     1741 aatcttcccg cgcagctgca cgggcttcac ggcgttcacg acgcagtaca ggatcgcgcc
     1801 ggaagccggg ctccacttca tcgctggcag caagttccgg attgtagcga cattcaggct
     1861 tcacccgttc aaatatatct gcacctgcaa tctcaaatgg ccctgcctgc ggctcagccc
     1921 tggataacgt caaatccggg tggatactgc tggccttcac tggtgtgagc tcatgattaa
     1981 acgcatccac aatcacaaga ccatgatgct gtttctgcaa cataagccct ttacgcgcaa
     2041 acatctcatg gcacgcctgc cagctgaaat tcccgcccgc gatcatatct tcaatcaacg
     2101 actcacactg aacagttgtg tacccccaca gactttcctt atcagaaaac agttcgcgct
     2161 ttattgctcc ctgtggacct gtccccatcc gcatacgata ccattcgtaa tcactgacag
     2221 cctgagtacc aagattacgt ccccggaacc gttctccggg tataacctga gtaaaaatat
     2281 ccttcggtac aggctgccat ccaccatcca gatcctgcat ataatccagc gtcattaacg
     2341 gccacacact gtctgcacgt actggtgtct gttgtttatc aaatccatca tgcagtacca
     2401 gatgcccgtg ctggatcccc atccataatc cagccgttgc cagagttctg tgcacatcca
     2461 ggcaactttc cagctcacca tttctcgcca tttccggtaa gcgttcggca aaatgacggc
     2521 aggcgtactg tttcagactc tccgtttctg ccactttttc cggccggata tcaacgttta
     2581 tggcttccgg atcataacga ccgggtgtcc ccacctgact gaaaatatct gtcggtacag
     2641 gctgatattc ccccagtttc cgccccagtt tttcagctgt ccataacggt ccgaacgaac
     2701 tgagtgcaac accttcccgc gctctgtccc agccatcctt caccaccagt tccccttcct
     2761 gcatcgtgat atacagacca tcactggcca gccgtttatg caaagataac cagtcctgtg
     2821 cgggctcttc acgcagcccg gcaatcgaca tctgagcaat atattccctg aacgtctgct
     2881 ttttcccgcg tcgccaggct gttcatgccc gtacaatgcc agcaaggaga aaggggccac
     2941 cggcgaacca gcagcccctt tataaaggcg cttcagtgca gtcagaccag catcagccct
     3001 gaaaaggcgg gcctgcgccc gcctccaggt tgctacttac cggattcgta agccatgaaa
     3061 gccgccacct ccctgtatcc gtctctgtaa cgaatctcgc acagcgattt tcgtgtcaga
     3121 taagtgaata tcaacagtgt gagacacact atcaacacac accagacaag agagctgcgt
     3181 ggtagtttca tggccttctt ctccttgcgc aaagcgcggt aagaggctat cctgatgttg
     3241 tctaagcatg caggggcctc gtgggttaat gaaaaattaa ctacggggct tttgtccttc
     3301 tgccacacaa cacggtaaca aaccaccttc acgtcatgag gcagaaagcc tcaagcgccg
     3361 ggcacatcat agcccatata ccagcacgct gaccacattc acttttcctg aaaataaccc
     3421 gctcattcag gctgtttacg ggcaatccgt gtgattgttg ccgcatcacg cacctcccgg
     3481 agtttgttcg gcaccatcca ccgctccatc tcacggacag catgaccggc ctcctttgct
     3541 gccagactgg cttccgcgat aacctgccag aatggcatat cctgccactg ccagcgttcc
     3601 attccggcca gtgacggaaa acgctcgccc cactgccaga acagggtttc caggtcatgc
     3661 tctgccaccc gatcactcca gcgtgcatac cagcttccga ctgtttccgg tgaatggaac
     3721 gccagttcga tttcagcctg ggcgaccagc gcctggtacg cacgggcttt ctgctctgcc
     3781 tccctcgcct gctggcggct gtacttctgc atgaccagcg cggatttatg gcgactgcgt
     3841 tccctgacac aaaaccgcac cgacgggaac aagcgttctg ctgcatcacc gggaagggga
     3901 aggaggcaga cctcgccctg tgtttcaatc agcacatcga tggcatacaa ccactgaaga
     3961 cggtttccgt gcagttcttc tgcggtcagg gacggcatga aaaatcgcag gtcagacagc
     4021 gatatcctgc ggcttccgtt cagacagcgg aaaaacgccc ccgcatacgg atattccgcc
     4081 agcgtgtttc cctgattgcg gcgtttttcc gtctctgcca ctgcccgcca tgcggcctgt
     4141 tgctctgtgc tgaggggaac cagtgcctgt gaacgtgcgc tcatacctga cctccttccg
     4201 ccatcaggat atggatgatg tcgttaacgc tgtatccctg cgtatccagt gacgccgcca
     4261 gtgccagtga atggctgacg gcttccggtg caaaccgccg ctgtacctga accaccgcaa
     4321 acggctcacc accggcattc accagaacca gcacccagac ctgggagaca tcgcccggtg
     4381 aacataacgc caggtgaaaa cgttcgtggg caggcgtgaa acggacctgg acggggaaaa
     4441 agccgccaaa ctcgctgccg tactcgccgt tcatcgtcca gttgtccggc gcatccagct
     4501 cccgcatcac ggcgtgtgtc agctcacggc gtgcatcact tccggcagcc cggatatctt
     4561 catattcctg tgcgttcatg gtatgtaata cattcagagt cagttcagtt ttcatcacgg
     4621 tatctccctt gctgccgccc cgtctccggc ggggcggtgt ggttgttcat caggcggcat
     4681 cagccaggtt atcggtgcgt tcagtggcat ccgtctgtgg gcgtggtgca cacatccagc
     4741 caggcaccca gcggttgtct ttcatatggt gctctgcatg ttcagccgca tcgcctttct
     4801 tcatcttctc cgcgtcccgt gcggcaccgg atagtcctgc ctcattcagg gctgcgataa
     4861 tctgcggctt tttcaggtgt ccgaaaaagt ttgctttcgt cggctgccac cagtcgcgca
     4921 tgtgaaagcc gatggcgctc tccagcgtgt ccagcggact gcgtgacgtg tgaccatact
     4981 cacgggtctg gacaccgtgg atactgcacg cggtgcagaa actgagcagt gataacagca
     5041 cctcctgact gagtgacagg aacgtcgtca tatcccgtgc ccatccatcc ggcagcaggg
     5101 cggcaagacg ggctttttct gccatcatcg ccatgaatgc ggcaccttcc ttccctgacg
     5161 gcgcatcgct ggtcagcgaa taatgctcac attccaggcg gattctggcg ggattgctgt
     5221 acgctccact gccaaataca ttcagacaga gtgtccatgc cagaagtgcc agggatttgt
     5281 ctgattgctg catcagtgcc gcctggactg ccagcgtgcg ctctgaggac attttcgtca
     5341 gcagcggcag actgatttcc tccactgagg ctttctcctg cacctgttcc gtgcggtaat
     5401 cgttatcagc cacatcatcc tcactgcgca actgcacacc acgctggaca cacacgttgc
     5461 cataacgcca ggacaccacc acgccacttc cggcacgcat ctccggcgtc cacgccctga
     5521 ccttcgccat gcagcgcatc agcttcattt cggcttccag caggtcggat tcctcacact
     5581 ggttttccag cgcatcgtaa cgtgccatca gttcgttcag gcgttcttct tctgcctccg
     5641 tcagcaccgc ttccggctcc ggcagactgc ggtatgttcc ggcatcctca cggcagaaac
     5701 caacaggctc catacgtccg gcgcaccatt cccagccttc ggcttcccga aggtgttcgg
     5761 cgacagcctg gagtttttcc agcagggcgg catcgagcgc cacgcagtcc acatagccac
     5821 cctcgtcgtc actgaacaaa tcggtgcgca gttcatctgg cgagaaggta tcagccccca
     5881 cgaagcggaa tttactgttc cctgccaccg ccacttcact ttcggtcacc agacgacgaa
     5941 tggtctgtac ttccggttta ccgccccatc ccgactgaca ggcggcttca aacacctgca
     6001 cctgacgcgc ggtgtcgttc tccagcgcca gcgcctgaca gtgctcggtg gtgatgcggt
     6061 cttctgccag cgcatcgagg atgacaggcg caaggtctgc cagtttcagc attcgctgaa
     6121 cgtggcgggg cgaataaccc agcaaatcac cgatttgtgc aggtgttttg ccttcctgcg
     6181 ccattgcacg gaatccggca atctgttcgg cagggtgcat atcccgacga tgaccgttct
     6241 cggtcatcga tgcggcagtc gccagctcct gcggaatgac cttcacacgg acaggccagt
     6301 cagccggaat gatgtcacgc tctgccagca tgttgagtgc tgccagtcgg cgaccacctg
     6361 cggcgacacc gtaacggtca cctggcaggg catgaacaac cagattctgt aacaggccga
     6421 cacctttaat ggagtccgcc agttcgctga cggactccgc agaatacggc accgtgcgca
     6481 cattcagcgg tgacttaatc agcgaggcca gcggcacgct cacttcctcc gtctgcgcca
     6541 gcagggccga caggactgtt tcctgcgttt ttgcaggttt acggctggat ttacgctccg
     6601 tttttgcctt agactctgta actgacatag ttagtacctc ccaaaacggt aataatgatt
     6661 gtctgccgga cgcgttcccg tccggcacct ctcttactta ataatcccga aatcacggat
     6721 gacatggttc atccagtacc cggcaccgtc ctcaaaattc caggtacgcc agaccattgc
     6781 accgttatta cggacaacca ggcgaaactg ttcgccgtgg tcattctcga taaagacgtt
     6841 ccggtactgt gcggcaacgg cttcggcatg tttgcgggta aaaggaccgt caggcagtcc
     6901 ctgaagtatt ctgaaatatt cgctcatcct catctctccg gtgatgcccc gcacaggacg
     6961 gggcggttgt cacagtcagt cgttcagaac gggatatcgt ctgaaaaccc gtaatcgtca
     7021 ccctccggcg gttgcggctg aggctgaccg ctgaactgtt gcccctcttc cggctgagtc
     7081 tgagcacctg cggcacgtac cagcatctgc atggtgcccg tggtcttaac aagaatttcg
     7141 gtgacgtaac gggtgatacc gttatcctcc cagctacggg tgcgaagctg accttcgatg
     7201 tagacctgcg cgcccttgcg cagatattca cctgccactt ccgcgagctt gccgaacagc
     7261 accacgcgat gccattctgt ctgctcccgc atctcccccg tctgtttgtc acgccagctt
     7321 tctgacgtgg ccacctgcag gtttgccact gcgcccccgt tggggatgta acggacttcc
     7381 ggatcttttc ccaggcgtcc tacgaggatg accttgttaa tgccacgaac tgccatgatg
     7441 tgtctccttc tgttgatgtt ttcagccccc atcatctttt gagtttcggg agccgcggtt
     7501 ttgacgtttt gcggggacct gccttttgtt ccctccggta ttcaccatca cctttttgag
     7561 aaccttcgcc gcaaggggca tcactgccac ggccggaaac gactgtccgg gggcagaccg
     7621 caaaactttt tgcctgcttt tgggcaaaaa gttttgcggg atgtgcattt cacccctgga
     7681 ctggcggaga cggttgtggc tgtctttgcc cccggcgaca ggggcttaaa caggtgaatg
     7741 gcaccggaca caacaacggg caggcacccg ccgcaacgac gtgaagcgcg gtttgcgaaa
     7801 cggcgttgcc ccttacatcc cggcggcctt ttgccgggat gccgggagcg caggccacgg
     7861 cgcgacagag gcggcgcgac cggaacggtc gctgcaagcc cgggtttcgc gtcagggatg
     7921 gaagcccgcc agggcggaga cagctccgct ggctccgttc acgacagccc gccccgccag
     7981 ggggacgccc ggaaaaggca taagcaggag aacaacaaaa cagacagtaa tcagagcagc
     8041 agaaaaaaga agaacagtag cagtagcagt agcagtagca gtagcatgaa caacaacatc
     8101 atcagtaaca gacaaatgac aggtctgaaa agacagaagc ccacagcaga atgtccgcgt
     8161 aatgtaaggg gcggcgcgtg agcgcggccc ctttcagcgc caccggcgcg cggtcttttc
     8221 ccggcgcggg cagacgggca tttatcctcc cgcctgcccg gagtcatcag gcacgcagac
     8281 gaataatcat cgtcggaaca tcggtataag caaacgtgcc gcgtggcagc tcctcgcgga
     8341 cgtcagaaaa cggtaacagc ttctcctgct ggcgcggccc gttcagacag acggcgacca
     8401 gcacgccacc cggacgcaac agggaaaagg cccgcaggat atgccggata tcctgcccgt
     8461 ggctgaacgg cggattaatg ataacccggc tgtaatactg caccggctgc cattccatga
     8521 agtcgccaca ctgaaccctg acaccgttaa aattttcccg cagataccgg accagcccgc
     8581 tgttgatttc caccgcatca cacatggcct ccggtgcggt atcccgaatc gcccgcagta
     8641 tggccccggt gccggcagag ggttccagaa tgtgatcacg gttgctgatc tccgccagcg
     8701 tcaccaggcg atcacagacc ggggcaggag tcacaaaaag ctgagattca gcccggattt
     8761 cagcaaccgg cgcggcgtta tccagccttg cgcgcatcag ccggatacgt tcttttaact
     8821 gactgtgcat gtcattatct ccgtcatccc ccggggattt cccgggggga tataccttat
     8881 ttctgtggga tttccgtaat tttcatgtcg gtgatataca cattcaccag acggaaatta
     8941 ttgtccattg tgcggcggta gcggtatgcc ccgtggtctt ctgcttctgc cacactgcgc
     9001 acggccttac agtcccgggg cagggcggcc cactgtgcct ttgtcatttc ccggaaacct
     9061 tcccccggat agttgactac cggcggacgc ttcgcggcct ggctggcgac ggcagcctct
     9121 tctgccgatg gtgctttgta gtccgtgatg cgatcgggcg tcactttcat cgtgccgctg
     9181 tacccgagaa aactgtaatt cggcgttgtg actgaactca ccgccccgtt gcttttgttc
     9241 acgcggatga tggtcagcca ctcgccccgg ctgaaaacct gtccgcccgg ctcaaaatcc
     9301 tgtgtccggg taaccacgcc gccgctttcg tccagcatgg cacgctcata gttcaggcgg
     9361 ttctgatagt ggttaaccca gcgttgctga tgctgaatct gccgttcatg acagcggatc
     9421 gcaatttcgc gggcctgctc cgtggtgatg atgtcatcat ccagggccga ccataacgac
     9481 cggctccctt catactggct tttttctgcc gggcgcggat acgtatccag cgggaaacag
     9541 gcgctgatat ggtcatgact gctgatgagt tttgccatat tcagatccag cgattcagcc
     9601 cgccacatcg tcagatattt ctgcgactgc gcgatggtct tttcagcctt gcgcaaatca
     9661 gcttcgattt ttttgatacg acgccagcga acgtccggac gttctttata cttcgcgtga
     9721 agcagtgctg accgcccccg ttcttcccag tattccgcac gttcaaagag catcacggca
     9781 cgtttcatgc cgttttcaat acgctgcgca tcacgacggg cgcggcgttc gctgtgatgc
     9841 cccacaagaa tgggctgacc gggcgggatc atcgcggcca gtctttccac ttcatcaagt
     9901 gcctgtgcgg attcactggc ccgctttccg ctgtatccgg taaaccgctc cgcccgtgct
     9961 tcctgacgtt cagcgagcgt gctgtcttca tcctcaatct ctccggcaag tgagaggagc
    10021 acgtcttccc ggcccggtgt ccaggccggc gcgacaaaca gcgcctgttt tggtgcccag
    10081 cgaaaaccgg catcatgcac ttttttatac gtctccgggt caagacgtga cacggcatac
    10141 aggcgcagtt tgttgtcatc cggtgagtag gtcgcgcggt acatctgcac agggctggcg
    10201 gcttccgggg cggtcatggt gattgctgct gatgtcataa aatgttctcc ttccgtctgt
    10261 tgccgcccct cgcggggcgg ctcacctcat cagttaaaag cgcgtttcag ttcgagtgtg
    10321 cggagcacct gcccgaaagc cgtgtttttc atggcgggga tgtgtacacc acgcccccgg
    10381 tgaaaactcc gggctatgtg tcgcccgaag gctttcagca cggggtcaat ttccggagcg
    10441 gggatgtctt cgcgtctgaa acggggatga cggcggggct ttacagccgc tgcggaaggt
    10501 gaaacaatgc ttacagtcat aaatggattc cttatgacaa attggttaac aatgccgtca
    10561 aaattggtcg cacaattctt acggcgtgga ttaagcaggt ccctgacctg cttttttcat
    10621 atctgcatct gacgctttcc ggtgttttcc tccttctgtt cctgcgttct cattacctgt
    10681 cagccggatg actgacagaa cacataaatg cgtattgccg gagcctgaaa taaatcctga
    10741 tttttaaaga acatcacccg ttgagtttcg ggagccgcgg ttttgacgtt tcgcgggaac
    10801 ctgctgctgt attccctgtg gtatgaactt cacccgtttg agaaccttcg ccgcaagggg
    10861 catcactgcc acggcctgac acacctgtcc tgggcagaac gcaaaatttt ttaccgggtt
    10921 ctgggtaaaa aattttgtgg gctgttcatt taccctggac agtcagtgcg gcaggttgtg
    10981 gctgtatcag ctcccggcga cagggactca tacgggtgtg tggcacccgg caggcaatga
    11041 gcagggaccc gccgcaacga cgtgaagcgc ggtttgcgaa acggcgttgc cccttacatc
    11101 ccggcggcct tttgccggga tgccgggagc gcaggccacg gcgcgacaga ggctgcgcga
    11161 ccggaacggt cgctgcaagc cagggtttcg cgtcagggat ggaagcccgc cagggcggag
    11221 acagcttcgc tggctccgtt cacgacagcc cgccccgcca gggggacgcc cggaaaagcc
    11281 ataagcagga gaggaacgga aagaagagga ggaagaggaa gaggaaaaag aagaagaaca
    11341 gtaacaggca cggtaaccac agaaaaataa catcagaaaa aaacattccg gacacatcac
    11401 aaacaggaag ggtaacggac gacgcgtcag cgtggcccgc ttcccttccg gcaggcgtca
    11461 gcctgccagg ggttttcgcg cgggctgaca ggcggcctcc accgccagcc ctgtatcacc
    11521 tgtttctgct gccctgtaag atgtagtgac cgcccaggaa aacagcctgc tgattcgcca
    11581 caaaatcgca gatttcatca acaatctgcg cggtggtgag cggtttctgc cccatttcac
    11641 gacgtcgcgc attaatcacg gctttgtaat tgcgcagaat ttcgcgggtt tctgtgctga
    11701 tattcatgac atgtgctccc cgtccagcgc ctgtaacaca acgtccattg cttcgcgttc
    11761 ctccctgacg aacgcatcac cgtcgccccc gttctcactc ccgacggcat acaggcgctt
    11821 catttcactg cctgccagcg ccatcacttt ttccagcatc accgccggaa ctgtcacctg
    11881 acggctggca cgcttttctt ccatcaactc acgaacaacg tcgtgaacaa tgctgacatc
    11941 cgcaccatat tcaaaggcat cgtccagccg ctgcatgacg gtttccagct catcgtctga
    12001 caggttccag tcctgggcta tgtggcgcac atccccacgg gtcagcacca cggcaaaaac
    12061 gcattcactg tccggcacgt ctgtatccag tacatcgcgg atgatttctt taacagtaca
    12121 gtacatgttc tcgtctcctt tacaggggcg gcattgccgc cccgtccgtt aatcagtcca
    12181 gtgccgcgta aatcgcgttg cattcagggt gaaattcgat gtgacgccac aactgcgcat
    12241 cgcgcatccg gtaaagctgg gtcagtcctg catcaccgct gtcgtggtac agccacaact
    12301 ggcggttaat cacaagggaa gtcagaatga tgccgcaggc atcagcactc acggtacggt
    12361 caaaccagtt tgcaccgttc accatgtgga aacggtcgcc atccggcatc atgtaaccgc
    12421 caccgtccgg caggcggatg aagtgccaga agcctccctg atattccggc atcatttttt
    12481 gcgccagcgc gtacacatac gcctcggcat acagaaaatc gttgccaaac aggtaaggca
    12541 gaaaggagag acggtcgcac tcttcaacat tcagagtgac aggtttcgca tattgcatga
    12601 tgttttcctc cgttgttcac atattccgcg acaccgggcg gtgccgtgcc tccagaaaag
    12661 ccgggcgata aggcggggat gcacagggcg caagggaagc cgcagcctga cgcacacggc
    12721 tgacgggagt tgcagggtga ggccgcgaaa cctgagcggg cgaacgaccg gaaaatcacg
    12781 gcagacggga aggagggacc gggtgaccgc agcggcgaag cgtacccttg catcaacgcc
    12841 agcccggcta agatggaggc acggcaccgc ccgcacacga cgctgaggcc acataactga
    12901 aaaaccacaa ggccatgccc tgcatggccg ggtaaagcgg agccagcggc gacttccttt
    12961 gtgctctgtc cggggaccgg caggaaaaag gcggggattt accccgccaa cgtcatcatt
    13021 tccggatttt tgccagggca tccgccatgc tgcggatttc gttccgggct tcccggacca
    13081 cctcccgcag aacaccacgg tcaaaccacc ggcggggcgg ttcatccggt atacgcagca
    13141 catcgcaggc cagcccactg gacaggatat gttcaataca atcgctgacc agatacattt
    13201 ccaccgccga aaaacgtgcg ccccggttca ggatggcatc gagcaccacg ggcaggtttt
    13261 cgtcgtcctc atcgagccag caggggtgat cgctttcctt cacgaccagg cgaaagcggc
    13321 gcgtctggcg ggtcagtaac gtctgaccgg tggtggtcag cgccgcccgg atacgccgcg
    13381 tatcttctgc cacccggcaa ctgacactgc gtaccgcatc gcgcaccgcc gcaagtacag
    13441 ccctgatttc gtcttcggtc agccattcac cggcatacgg aaaagacacc tgcgttccgt
    13501 ctgccggaag caccggcagc gactgccact gactgccgga cagaacaagt tccgctccgg
    13561 ttccggtcag tttctgaatg gcggaggcca ggtgcggcgg cagtacgccg ccgtggcggt
    13621 taacgagtaa cgtcgccagc cggggcgcat ccagcagaac ataaatgctg atccagcccg
    13681 gatacttcgg gttacgctgg tcgacatccg tctcttccgg ccagccctgc gccagcagca
    13741 ggttacgggc gtgacgacaa atcagtgcgt tcagcgttgt gttttcgttc atgattaatc
    13801 gttctccctg tatcgccgcc cgcaggcggc gataacatca gccgttttcg tgtgtgttgt
    13861 ttttcagcag gatgcgggcc tgattaacca ccgcctgacg gcgggtaacg cattccggga
    13921 cggcgagacc gtaatcacgg gcggcccatt cacgtttcca cagtgcctgt tcggcggtct
    13981 gaatcaggga cgccagcagt tcagccggga cggtcacctg gcggttttcg ctcacgttgc
    14041 tgataatctc catcgccgcg gcagaagaaa taccggactc aattcgctgg tcttccggga
    14101 tgtcctccag gcgcgccagg acggtcctga tttcaggatc ggacagggaa atatccattc
    14161 cgtcagcgag ggcctgaatc tcttccgggg accagataac cagcatcagc ggtgtatatc
    14221 ccggatactt tgctgccagc tcccggcata gcgtttcgca tgttccgtac attctcgtct
    14281 ccttctcaat ctttaaagca cgcatccagt tccagaattt cggtatacgc ctccatgaac
    14341 aggcgattgg cgcgatacag cgtctggcgt gccaggcgtt caacaagcac atcggaatca
    14401 aagcgacagc gatgctccat atcgttcagc ctggcccgaa ggtcacacat ggtgttggcc
    14461 acctccgcca cgtcggcgcg aagtttttca tgtccggcat agttcattta cgcagcctcc
    14521 ggcataaacc agtcatcatt tgcggccccc tgctgcatgg cccgctgtac ggcagccaga
    14581 cgttgctgtc cggcacggtg atactgttca agcagctcga taccgatata ccggcgtccg
    14641 gactgaaggg cggccacaca ggttgagccg ctgcctgcaa acgggtccag cacaatcgcg
    14701 ttcgggtgtg tgaagctctc aatcagtggt tgcaggctgg taacaggctt ttccgtcggg
    14761 tgatgacgat tgcctgaata tttccagccc agcacgtcgg gcaatggctt ctgaggcagc
    14821 gccggacgcc cttttgccag gatgtaagcg cattcgtggc gatagcccac atatgcggcc
    14881 ttcgaagtgt aggttttggt aaaaaccagg tggccaacca cgctgaatcc cgcatttttc
    14941 caggcggcca taaagcgatc gacgcggttc cagccataga agctcaccat cagcgcatct
    15001 tttttcagca cgcggtacat ttcattacag gccggttgca gccattcatc ggttttatcg
    15061 ccggcgatgg tacgcccctg acggtcacgg aaaccgacga gatatggcgg gtcggtgagg
    15121 atgaaatcga caccattgcc cggaatcgtg gccataacac gcacgcagtc accctggata
    15181 aaacgggaca taacagcact ccttctctta cagagggcac cgggagggcg cccctgtggc
    15241 gcccgttccc cgcggcctgt gggctgttca ccgcgtccgg taacgggtca ggccgaccgt
    15301 ggaatatcgc agccgtcgtc tttttgacgg cgagaatatg ccgcgaaagc ggcttgaagg
    15361 gcgttcatca gaaaagggcg acgggctggc gtacactgtc accgatgcca gccgggagcg
    15421 gtaaaagcgc ccgtcccgtg cccggccgga cgcgggacac tgaccacagg cagcgttcct
    15481 gccgcgccgc gtcagccggc gcagtaaaaa gaggagcccg gcgacatcct ttaccggaaa
    15541 agaaacgggc ggttgcccgc ccgtctccgt catacttcat ccaccgtaat gcgccacctt
    15601 gtcgactatc cactccggcg cggtgacttc cggcagctca tcatcatctg tcggggaaga
    15661 ccattccagt tccccccaca gcacatcaac aagctctcca cgctcatatc gctgccagcc
    15721 acagaaattg cagccctgcc cggcgtacca gtgctccagc atgcaactga aacggcggct
    15781 taatactgca ataacgtcgc tttccggctg acaccacggc gtgtcgaaat caacctgaat
    15841 gaagttgtct ccctgactgc tgatattcac ctcataccca caaggccact tcacgccata
    15901 ctgttccgta taccagtgat atgcagaagg aacaccgttc agaacaccac cgttaaagcc
    15961 attcacttca acatccaggc gagtggacag aaccgtcagc aggtcaaacg gcatggtttt
    16021 ttccggcagg acgttgtcac acagacggtt ccaccacaca gatacatcct cgttactcca
    16081 gatgtcgcac cagtcacctc tttttggggt gaaaagagcg gtaatgcttt ccctggcatc
    16141 atccggtaat tcttcccagc gacgccggcc agtaccgctc tgcagccaga gttcatgcag
    16201 catcaggcag ttttgctcat ccagaagtac accgtcctgc aggtgtgtca gccagcgggt
    16261 gaacgcgata ttctccggcg atacaacgcc acgcccggca gccgtcagtc cggggcatgg
    16321 ttcaaaccgc acatcttcag tggtctgcag aagtccggca cttccggcca gaaacagctg
    16381 aataccttca tttgtggcgc ggcgataaag cggtgtgact gcaccgctgg ccagtcgttt
    16441 aatctcagcg atctgtgccg gttcaccaga aaaatacata cgattcgagc accagtttgg
    16501 catgggaatt ctcctctcac atgcggcctc catcaggccg tgccgcacct gtaaccgggc
    16561 gaccgcatac gaatgcaggt gcggccccga agccgcagcg cgtggaggag gaacgacgaa
    16621 acgtgaccgg gtgaggaggc cggcgacagc gacacacttg catcggatgc agcccggtta
    16681 acgtgccggc acggcctggg taaccaggta ttttgtccac ataaccgtgc gcaaaatgtt
    16741 gtggataagc aggacacagc agcaatccac agcaggcata caaccgcaca ccgaggttac
    16801 tccgttctac aggttacgac gacatgtcaa tacttgccct tgacaggcat tgatagaatc
    16861 gtagtctcac gctgatagtc tgatcgacaa tacaagtggg accgtggtcc cagaccgata
    16921 atcagaccga caacacgagt gggaccgtgg tcccagaccg ataatcagac cgacaacacg
    16981 agtgggaccg tggtcccaga ctaataatca gaccgacgat acgagtggga ccgtggtccc
    17041 agtctgatta tcagaccgac gatacaagtg gaacagtggg cccagagaga atattcaggc
    17101 cagttatgct ttctggcctg taacaaagga cattaagtaa agacagataa acgtagacta
    17161 aaacgtggtc gcatcagggt gctggctttt caagttcctt aagaatggcc tcaattttct
    17221 ctatacactc agttggaaca cgagacctgt ccaggttaag caccatttta tcgcccttat
    17281 acaatactgt cgctccagga gcaaactgat gtcgtgagct taaactagtt cttgatgcag
    17341 atgacgtttt aagcacagaa gttaaaagag tgataacttc ttcagcttca aatatcaccc
    17401 cagctttttt ctgctcatga aggttagatg cctgctgctt aagtaattcc tctttatctg
    17461 taaaggcttt ttgaagtgca tcacctgacc gggcagatag ttcaccgggg tgagaaaaaa
    17521 gagcaacaac tgatttaggc aatttggctg tgttgataca gcgggtaata atcttacgtg
    17581 aaatattttc cgcatcagcc agcgcagaaa tatttccagc aaattcattc tgcaatcggc
    17641 ttgcataacg ctgaccacgt tcataagcac ttgttgggcg ataatcgtta cccaatctgg
    17701 ataatgcagc catctgctca tcatccagct cgccaaccag aacacgataa tcactttcgg
    17761 taagtgcagc agctttacga cggcgactcc catcggcaat ttctatgaca ccagatactc
    17821 ttcgaccgaa cgccggtgtc tgttgaccag tcagtagaaa agaagggatg agatcatcca
    17881 gtgcgtcctc agtaagcagc tcctggtcac gttcattacc tgaccatacc cgagaggtct
    17941 tctcaacact atcaccccgg agcacttcaa gagtaaactt cacatcccga ccacatacag
    18001 gcaaagtaat ggcattaccg cgagccatta ctcctacgcg cgcaattaac gaatccacca
    18061 tcggggcagc tggtgtcgat aacgaagtat cttcaaccgg ttgagtattg agcgtatgtt
    18121 ttggaataac aggcgcacgc ttcattatct aatctcccag cgtggtttaa tcagacgatc
    18181 gaaaatttca ttgcagacag gttcccaaat agaaagagca tttctccagg caccagttga
    18241 agagcgttga tcaatggcct gttcaaaaac agttctcatc cggatctgac ctttaccaac
    18301 ttcatccgtt tcacgtacaa cattttttag aaccatgctt ccccaggcat cccgaatttg
    18361 ctcctccatc cacggggact gagagccatt actattgctg tatttggtaa gcaaaatacg
    18421 tacatcaggc tcgaaccctt taagatcaac gttcttgagc agatcacgaa gcatatcgaa
    18481 aaactgcagt gcggaggtgt agtcaaacaa ctcagcagga gtgggaacaa tcagcacatc
    18541 agcagcacat acgacattaa tcgtgccgat acccaggtta ggcgcgctgt caataactat
    18601 gacatcatag tcatgagcaa cagtttcaat ggccagtcgg agcatcaggt gtggatcggt
    18661 gggcagttta ccttcatcaa atttgcccat taactcagtt tcaatacggt gcagagccag
    18721 acaggaagga ataatgtcaa gccccggcca gcaagtgggc tttattgcat aagtgacatc
    18781 gtccttttcc ccaagataga aaggcaggag agtgtcttct gcatgaatat gaagatctgg
    18841 tacccatccg tgatacattg aggctgttcc ctgggggtcg ttaccttcca cgagcaaaac
    18901 acgtagcccc ttcagagcca gatcctgagc aagatgaaca gaaactgagg ttttgtaaac
    18961 gccaccttta tgggcagcaa ccccgatcac cggtggaaat acgtcttcag cacgtcgcaa
    19021 tcgcgtacca aacacatcac gcatatgatt aatttgttca attgtataac caacacgttg
    19081 ctcaacccgt cctcgaattt ccatatccgg gtgcggtagt cgccctgctt tctcggcatc
    19141 tctgatagcc tgagaagaaa ccccaactaa atccgctgct tcacctattc tccagcgccg
    19201 ggttattttc ctcgcttccg ggctgtcatc attaaactgt gcaatggcga tagccttcgt
    19261 catttcatga ccagcgttta tgcactggtt aagtgtttcc atgagtttca ttctgaacat
    19321 cctttaatca ttgctttgca tttttttatt aaatcttgca atttactgca aagcaacaac
    19381 aaaatcgcaa agtcatcaaa aaaccgcaaa gttgtttaaa ataagagcaa cactacaaaa
    19441 ggagataaga agagcacata cctcagtcac ttattatcac tagcgctcgc cgcagccgtg
    19501 taaccgagca tagcgagcga actggcgagg aagcaaagaa gaactgttct gtcagatagc
    19561 tcttacgctc agcgcaagaa gaaatatcca ccgtgggaaa aactccaggt agaggtacac
    19621 acgcggatag ccaattcaga gtaataaact gtgataatca accctcatca atgatgacga
    19681 actaaccccc gatatcaggt cacatgacga agggaaagag aaggaaatca actgtgacaa
    19741 actgccctca aatttggctt ccttaaaaat tacagttcaa aaagtatgag aaaatccatg
    19801 caggctgaag gaaacagcaa aactgtgaca aattaccctc agtaggtcag aacaaatgtg
    19861 acgaaccacc ctcaaatctg tgacagataa ccctcagact atcctgtcgt catggaagtg
    19921 atatcgcgga aggaaaatac gatatgagtc gtctggcggc ctttcttttt ctcaatgtat
    19981 gagaggcgca ttggagttct gctgttgatc tcattaacac agacctgcag gaagcggcgg
    20041 cggaagtcag gcatacgctg gtaactttga ggcagctggt aacgctctat gatccagtca
    20101 attttcagag agacgatgcc tgagccatcc ggcttacggt actgacacag ggattcgtat
    20161 aaacgcatgg catacggatt ggtgatttct tttgtttcac taagccgaaa ctgcgtaaac
    20221 cggttctgta acccgataaa gaagggaatg agatatgggt tgatatgtac actgtaaagc
    20281 cctctggatg gactgtgcgc acgtttgata aaccagggaa aagattcata gcctttttca
    20341 tcgccggcat cctcttcagg gcgataaaaa accacttcct tccccgcaaa actcttcaat
    20401 gcctgccgta tatccttact agcttccgca gaggtcaatc cgaatatttc agcatattta
    20461 acaacatgga tctcgcagat accgtcatgt tcctgtaggg tgccatcaga ttttctgatc
    20521 tggtcaacga acagatacag catacgtttt tgatcccggg agagactata tgccgcctca
    20581 gtgaggtcgt ttgactggac gattcgcggg ctatttttac gtttcttgtg attgataacc
    20641 gctgtttccg ccatgacaga tccatgtgaa gtgtgtg
//
LOCUS       DBFIFM010000069        20536 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.069, whole genome shotgun sequence.
ACCESSION   DBFIFM010000069 DBFIFM010000000
VERSION     DBFIFM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.069"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            82..141
                     /gene="ibsE"
                     /locus_tag="N7C74_002212"
     CDS             82..141
                     /gene="ibsE"
                     /locus_tag="N7C74_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HGU2432075.1"
                     /translation="MMKLVIILVVLLLLSFPTY"
     gene            complement(261..1694)
                     /gene="hldE"
                     /locus_tag="N7C74_002213"
     CDS             complement(261..1694)
                     /gene="hldE"
                     /locus_tag="N7C74_002213"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HGU2432076.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVVA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1742..4582)
                     /gene="glnE"
                     /locus_tag="N7C74_002214"
     CDS             complement(1742..4582)
                     /gene="glnE"
                     /locus_tag="N7C74_002214"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HGU2432077.1"
                     /translation="MKPLSSPLQQYWQTIVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SISAHPEWLTELESQPPQADEWQHYVAWLQEALINVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRWELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLLRITALLAGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(4605..5906)
                     /gene="ygiF"
                     /locus_tag="N7C74_002215"
     CDS             complement(4605..5906)
                     /gene="ygiF"
                     /locus_tag="N7C74_002215"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HGU2432078.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6148..6768
                     /gene="ygiM"
                     /locus_tag="N7C74_002216"
     CDS             6148..6768
                     /gene="ygiM"
                     /locus_tag="N7C74_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HGU2432079.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6832..8070
                     /gene="cca"
                     /locus_tag="N7C74_002217"
     CDS             6832..8070
                     /gene="cca"
                     /locus_tag="N7C74_002217"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HGU2432080.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFTTLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(8251..9072)
                     /gene="bacA"
                     /locus_tag="N7C74_002218"
     CDS             complement(8251..9072)
                     /gene="bacA"
                     /locus_tag="N7C74_002218"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGU2432081.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTSGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9163..9531)
                     /gene="folB"
                     /locus_tag="N7C74_002219"
     CDS             complement(9163..9531)
                     /gene="folB"
                     /locus_tag="N7C74_002219"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HGU2432082.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DNVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9637..10254
                     /gene="plsY"
                     /locus_tag="N7C74_002220"
     CDS             9637..10254
                     /gene="plsY"
                     /locus_tag="N7C74_002220"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HGU2432083.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10267..11199)
                     /gene="ttdR"
                     /locus_tag="N7C74_002221"
                     /gene_synonym="dan"
     CDS             complement(10267..11199)
                     /gene="ttdR"
                     /locus_tag="N7C74_002221"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HGU2432084.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVYFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            11406..12317
                     /gene="ttdA"
                     /locus_tag="N7C74_002222"
     CDS             11406..12317
                     /gene="ttdA"
                     /locus_tag="N7C74_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HGU2432085.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATIKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            12314..12919
                     /gene="ttdB"
                     /locus_tag="N7C74_002223"
     CDS             12314..12919
                     /gene="ttdB"
                     /locus_tag="N7C74_002223"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HGU2432086.1"
                     /translation="MKKILTTPIKAEDLQDIHVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPLPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12968..14431
                     /gene="ttdT"
                     /locus_tag="N7C74_002224"
     CDS             12968..14431
                     /gene="ttdT"
                     /locus_tag="N7C74_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HGU2432087.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14474..15487)
                     /gene="tsaD"
                     /locus_tag="N7C74_002225"
     CDS             complement(14474..15487)
                     /gene="tsaD"
                     /locus_tag="N7C74_002225"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HGU2432088.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15725..15940
                     /gene="rpsU"
                     /locus_tag="N7C74_002226"
     CDS             15725..15940
                     /gene="rpsU"
                     /locus_tag="N7C74_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HGU2432089.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            16051..17796
                     /gene="dnaG"
                     /locus_tag="N7C74_002227"
     CDS             16051..17796
                     /gene="dnaG"
                     /locus_tag="N7C74_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HGU2432090.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17991..19832
                     /gene="rpoD"
                     /locus_tag="N7C74_002228"
     CDS             17991..19832
                     /gene="rpoD"
                     /locus_tag="N7C74_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HGU2432091.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(19911..20417)
                     /gene="mug"
                     /locus_tag="N7C74_002229"
     CDS             complement(19911..20417)
                     /gene="mug"
                     /locus_tag="N7C74_002229"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HGU2432092.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDHQLKPQEAQHLLDYRCGVTKLVDRPTVQANEISKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLSIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT         4859 a         5728 c         5263 g         4686 t
ORIGIN      
        1 tcatcaacaa tcggggagta agcaaagggc tgaaacggga aagcccctcc cgaggaaggg
       61 gccttataga aggaaagggt tatgatgaag ctcgtcatca tactggttgt gttgttactg
      121 ttaagtttcc cgacttacta acaactcatc agagggggga gaaatcctcc cttacccttg
      181 ttcctttact ctaggttgaa aagacaacag cgtcaatagg cctgccatgt acgaagcgag
      241 atctgtgaac cgctttccgg tcagcctttt ttatcctgtt ggatcttctt gataatgtta
      301 gtggtcgagc aaccgtcttc aaagttgagc accaacactt cgccgccgtt ggcccagact
      361 tctttactcc cggcgatctc ttccggtttg tagtcaccgc ctttcaccag caggtctggc
      421 aggatcccgg cgatcagacg ctgcggcgtg tcttcttcaa acgacactac ccagtcgacc
      481 gcttccagtg cgcccagcac aatcatacgc tgttcgagtg ggtttaccgg gcgggaatcc
      541 cctttcagcc gtttggtgga ggcatcgctg ttgacggcaa caatcaagcg gtcacccagc
      601 ttgcgggcat ttgccagata agagacgtgc ccggcatgta ggatatcgaa gacaccgttg
      661 gtcattacca ctttttcacc acgtttacgc gctgccacta cagccagctt cagttcctct
      721 tcggtcatca cgccaaagcc ggtatctgcg cgtccacgta cagcgttttc cagctcgatc
      781 ggcgaaaccg tggacgtccc cagtttgccg accaccacac cagccgccgc attggcaaag
      841 aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac gccaatcacc
      901 gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gagttggcat atgcagcggc
      961 gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa cagagccgag
     1021 agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc ggtcttacat
     1081 ttaccgacaa cagcttcaaa ttccgagaga ttcggcgtta acagcgtagc gccgcggtag
     1141 cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cacccgcttt acgcgccagt
     1201 tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag caccagcgcg
     1261 ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc aacaccttcg
     1321 aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac ccgtaatttg
     1381 gtaatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac gtcggccaga
     1441 gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag gcgtgcatta
     1501 gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg ttcttcgatg
     1561 gtattcactt taaccacggg caccggcgct tccggcgaga tacgactgga ggggccgtac
     1621 cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc aaactctggc
     1681 agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat aataccatac
     1741 ttcattcttc caccagccac ttctgccagc ttgcccgcac cagttcacgc tctgcggtga
     1801 agcaatcctc cggcacatgg cccggcaatt cctgtaatgc cagatgatga agttcatcgc
     1861 gaagcgtagt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata atgtcgtttt
     1921 gcgccagtag ttccagaatg cgcacgttgt ctgaccagcg cgttaacttc ggtttttcat
     1981 gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt cccccttcat
     2041 cagctttgat atcaaagcga tcgcgatgtt tattgccgag atgagcgcgc attttctcgc
     2101 gcatttcccg cacttccgtt tgcagagttt taccttcacg cggcagcgtc ataatctcgc
     2161 gacgcactgc gtcaaagtgc gcggtgagct gcggatcgcc gtacactaca cgcgcacgca
     2221 ccagcgcctg atgctcccac gtccaggcct cgtttttctg ataatcggca aatgcttctg
     2281 tggaggtaac cagcattccc gccgccccgg acgggcgcag ccgcgcatcc acttcgtaca
     2341 aaatgccgga agaggttcgc gtgctgaaca ggtgcataat gcgttgcgcc agacgcagat
     2401 aaaactgtcg tccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt gggcaatcat
     2461 ggaggaagat caggtcaaga tcggaactgt agcctaactc ccagccgccc agcttgccgt
     2521 agccgaccac tgcaaaacca cgcccttcgc gttcgttcag gtgattcggc ttaccgtagc
     2581 gggcaaccat ttgaacccac gcctgctgaa cgacggcatc tatcatggct tccgccagcc
     2641 aggttaagtg atcgctcact ttcatcaccg gtagcgtacc ggcgatatcc gctgcggcga
     2701 tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgc tcttcgtcat
     2761 cttccggcac gcgcagcaaa tactggcgca actcatcgcg gtaggcatcg gtcgccgtcg
     2821 gctggtaaag ggtgtttgga tcgagcaatt catccagcaa taatggataa cgcgccagct
     2881 ggctggcaat catcggcgac gctgcacaca gagaaatcaa atgtttaagc gccgcgggga
     2941 attcactgag caattctaaa taggtggtgc gggtaacaat ccccgccagc aaggcggtaa
     3001 tgcgcaacag cgtaacggca gcgtcttcac gcgcacagac atcacttagc agatgcggca
     3061 tcagatggtc gagcacctga cgcccgcgtg gcccgattgt gcgcttatcc agctctttgc
     3121 ggaaatcggc aatcagcgtc agcacctgtt tgcgatcgtc ctcgctaaga tgcgccagca
     3181 ctggtgtagt gtcatcttcc tgcaacgcat cctgccacag ctcgcgccac tgttccgaca
     3241 gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac acccggcgca
     3301 cattggtcat atgtgcggtc agcgccccgg tcaattgcgg ccagtcagca aagtccatcg
     3361 cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtctgggtt tgttcgtcgt
     3421 taatgctttg cagcaggttt tccagacgcc gcaggaacag atacgccact cgcaattgtt
     3481 cagcatcgtt ttcagaaagc agatgcagcg cggcaatgac gctgagcgtt ggcagtaaag
     3541 agcgcgactg cagcgacggt tcgcgcccgc cgcgaatgag ctggaacacc tgaacgataa
     3601 attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa ccacgtcgac
     3661 gcacttcacg ggcaatcatt cctttcatgt tgcgcagcga ctgaatcacg ctgaaatcga
     3721 tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca tagacgcctt
     3781 cgctatcgcc cataatccgc gccttgacca tcgcgtaacg ctcccagtcg cgcccctgct
     3841 cctggtaata atcttccaac gcggcaaagc tcagcaccag cgggccactt tcgccaaacg
     3901 gacgcagccg catatccacg cgatagacga agccatcctg cgttggttga tccagcactt
     3961 taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc cagcgtccac
     4021 cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag gagaaattca
     4081 gttccccgcc gcccagctta cccatgccta aaatcagcag cggttgcgct tcgccctgcg
     4141 cattgcacgg cgttccccac tcgcggcagc aggcgtcata tagccagtca cgcgccgcaa
     4201 caatcagcgt ctccgccaga tagctgagct gctgcaatat gctctcttca gtaaccagtg
     4261 ccagcgtttg cgcccaggcg atacgtacca tgatgcgccg acggaacagc cgcagctcgc
     4321 gcattaaccc ggcttcgtca ctcacattaa tgagcgcctc ctgcaaccat gcaacgtaat
     4381 gctgccattc gtcggcctgc ggcggttggc tttccagttc cgtcagccac tctggatgcg
     4441 cacttatgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc tgtgcactaa
     4501 gtgattcctc ggctaaaggc tctggcagcc gctcaacaat ggtctgccag tactgctgta
     4561 acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg ttttccgctg
     4621 tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat ttcgatgcgt
     4681 tgcccggtcg caatagcgtg atgcagcccc tgccagttct ccagccaggc ttgcgcgacg
     4741 acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcgt caggcgtggc
     4801 aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag ttcagcggca
     4861 tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctgatttt gccctgcgct
     4921 ttggcatcta aaaatggctg ccatgctttg cttaccaacc attcggtcaa cgccagcttc
     4981 gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga agcaatggtc
     5041 gcttcgcatt gggtcagcaa atcacgtaag tgagtgctcg ctttacgcgg cacaatacca
     5101 ccgaacagca tcagcgtatg acggaccaag ctaatggctg ccagcacctg ttctttcgcc
     5161 gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa cgccagctcg
     5221 agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa aatggtggtc
     5281 ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc cgctttgctc
     5341 aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt cagcaccgcg
     5401 cgcgtgtcgc cgctaagcag ctccagttcc agctcacaga taggttcagc aaattccccc
     5461 gctttcacct caccctgatc gagagcgatt tcaattcggc tgccatcgac cgccaccagc
     5521 catttttcgc gataaaaatc ggtgctgaac agcggttgta cgcgggaagc gagatcggcg
     5581 ggcaattcgc cgttcggcca gacttccgtc ggtaactgcg ccaggtcgag cgtcggttcg
     5641 ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct tcctgcaact
     5701 ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat atcgtgccca
     5761 cgcagccagt tatccggcgt ttcgtagtaa atattcagta actgcacggg gtcatggtgc
     5821 tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcgctgtg attaacaata
     5881 aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca cataagtgac
     5941 gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg cgccaaattg
     6001 ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga cctgacacag
     6061 aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta ctatcgttcc
     6121 actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg attaacttta
     6181 ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt ttctgacgaa
     6241 ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg cacggttaac
     6301 gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc ccaggtgaaa
     6361 gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga gccaagcctg
     6421 cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa actcaccaat
     6481 atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc gcagagcgac
     6541 agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct gattgtcgcg
     6601 cagaaaaagg tcgatgccgc cagcgtgcag ctggatgaca aacagcgcac catcatcatg
     6661 caatggttta tgtatggcgg cggcgtgctg gggcttggct tgctgctcgg tctggtactg
     6721 ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat cgccttcttc
     6781 gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg cgtgaagatt
     6841 tatctggtcg gtggtgctgt tcgggatgca ttgttagggc taccggtcaa agacagagat
     6901 tgggtagtgg tcggcagtac gccacaggag atgctcgacg cgggctacca gcaggtaggc
     6961 cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct ggcacgtacc
     7021 gaacggaaat ccggctccgg ttacaccggt tttacctgct atgccgcacc ggatgtcacg
     7081 ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca ggacgataac
     7141 ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct gttgcgccat
     7201 gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg ttttgctgcg
     7261 cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat gcgcgagatg
     7321 acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga gacagaaaac
     7381 gccctcacca cccgcaatcc acaggtgttc ttccaggtac tgcgcgattg cggcgcactg
     7441 cgcgttttat tcccggaaat tgacgccctg tttggtgttc cggcccctgc caggtggcat
     7501 ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc gatgctgagt
     7561 ccgcaggtcg atgtccgttt cacaacttta tgccacgatc tcggtaaagg actgacgccg
     7621 ccagaactct ggccgcgtca tcatggtcat ggcccggcgg gtgttaagtt agtggaacaa
     7681 ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact ggtggctgag
     7741 tttcacgatc tcatccacac cttcccaatg ctgaacccaa aaaccatcgt caaattattt
     7801 gattccatcg atgcctggcg taaaccgcag cgtgtcgagc aactggcgct gaccagcgag
     7861 gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg ccgctggttg
     7921 cgcgaagcct gggaagtggc gcagtcagtg ccgacaaaag ccgtcgttga agcgggattt
     7981 aaaggtgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt agccagctgg
     8041 aaggaacaac gttgcccaaa gcctgagtga gtattgggca ttttgccgga tgcggcgtaa
     8101 acgccttatc cggctacatg tcaacgacag ttgtaggcct gataagacgc gcaagcgtcg
     8161 catcaagcgt tgattgccgg acgcggcgtg aacgccttat tcggcctacg caatcgtgga
     8221 caacgccagg catccgacac tattcctcaa ttaaaagaac acgacataca ccgcagccgc
     8281 cacaataaag cgataaatgg cgaacgggat aaacgaaatg cgcttaatca attgcaggaa
     8341 ggttttaatc gctatcagcg ccaccacaaa agcggtgata aacccaacgg caaacatcgg
     8401 gatatcgccg cttgtcagga agccccagct tttgtagaga tcgagcgccg ttgcgcccat
     8461 catcatcggc accgccagca ggaacgaaaa ctcggaagca gcgtaacggc tcacccccat
     8521 cagcatccca cctgaaatgg tcgccccgga acgggagaaa cccggccaca gcgccagaca
     8581 ctggaaacaa ccaatcataa acgcctgacg ataggtcata tcatcaagac ccggcgcacg
     8641 cggctctttc ggcttcaggc attcggcggc aatcagcaac aaaccgccaa cgaccagcgc
     8701 atacatcaca tttatcgggt taaacaatga cttaatcgtg tcgtggaaca acagccccag
     8761 taccaccgcc ggaatcatcc ccagcaaaat gtggatcagc gttaaacgac ctttgctttc
     8821 tccttcgtgc tgcaacgggc ggccaaagtg gatgccaatc aggccaaaca gacgccgcca
     8881 gaacatcact actaccgcca gaattgatcc taactggatc acaacttcaa aggttttcgc
     8941 cgtgtcgccc tcaaacccca acaagtgacc gacaataatc atatggcccg tgctggatac
     9001 cggcagaaat tctgtcaatc cttcgaccac acccaatatt gccgctatca gcagcgagtg
     9061 catatcgctc atcaataaac ccctaaatta taaaaatgta acgcttgtcc gaactactgc
     9121 agtatgacca ggttataacc gtttggttta acagctgtaa aattaattat tttctttcag
     9181 attattgcca cgctcaatga ttacgccaac attcgccgcc cgcgccactg cgcctggctt
     9241 gctgagtttg atacgcaccc acggcgagtt gaagcgtgct aacagcagct ctgccacctc
     9301 ttccgccacg cgctccacca gcgcaaaacg tgccccctcg acgtggctga ccaccgtttc
     9361 tgcaatgtca gcgtagctga ggcaatccgc cacattatcg ctttttgccg ctttacggtt
     9421 atcccacgcc atttcgatat cgaacactaa tttctgttcg atggtctgtt cccagtcgta
     9481 aacaccaata gtggtgatta ccgaaagttg ctctataaat acaatatcca tcacgacctg
     9541 ctcgcttttt ggctaacccg gataccactt ccggcgaaat gtgcgtatta tccacagatt
     9601 catcgtttaa cacgaatttt caaaacggaa cagcttatga gtgcaatcgc gcctggaatg
     9661 atcctcatcg cgtacctctg cggctccatt tccagtgcca ttctggtttg ccgcctgtgt
     9721 gggctgcccg atccgcgaac cagcggctcc ggtaatcctg gcgcaactaa cgtgttacgt
     9781 ataggtggca aaggagcagc cgtagcagta ctgattttcg acgttctgaa aggaatgttg
     9841 cccgtctggg gcgcgtatga attaggtgtc agccccttct ggctaggctt aattgccatc
     9901 gccgcctgtc ttggacacat ctggcccgtt ttcttcggat ttaaaggagg aaaaggcgtt
     9961 gctaccgctt ttggtgccat cgcacccatt ggctgggatc tcaccggagt aatggcggga
    10021 acctggttac tgaccgtgct attgagcgga tactcgtcgc tgggagcgat tgtcagtgca
    10081 ctgattgctc cgttttatgt ctggtggttt aagccacaat tcaccttccc ggtttcgatg
    10141 ctctcttgcc tgatcctgct gcgtcatcat gacaacatcc agcgtctgtg gcgtcgtcag
    10201 gagacaaaaa tctggacgaa attcaaaaga aagcgcgaaa aggatcccga gtgatttctg
    10261 gtggatctac atgacctgat agccttcatc gggtttgccc agccgttgct ggcaccatgc
    10321 cgccagaaat tccacgcaga cacgtaattt catgctgcga tagagcggct cccggtaaac
    10381 agcccagata ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc
    10441 cagaaacggc aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca
    10501 ttgcagcaca atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt
    10561 ctcctgaccg ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag
    10621 acagtcatga cgacttaatt cctgtaggga ttgtggctgc ggatattttt gcagatactc
    10681 aggtgctgcg cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg
    10741 aatttcgtca ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg
    10801 atcgaacaat tcaaaataca cctgtaactc aggataattg cgcataagtt cggtaatggc
    10861 tggcgcaata tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg
    10921 gcgcgttttg atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag
    10981 cgcatgttcg taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt
    11041 cagcagcgtt gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg
    11101 cgtttgccca agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag
    11161 tacctgcaag tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg
    11221 ataagcgcag tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct
    11281 tattttcgcg ttgattcacg aactgttaat tacatattca cggtggcaaa aaatataaaa
    11341 ccacattttt agtggtagtt tgtggcggtg aatttttcca gacaaataca aaaactggag
    11401 ttgccatgat gagcgaaagt aataagcaac aggcagtgaa taagttgacg gagattgtcg
    11461 ctaactttac cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac
    11521 agctaaagga tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca
    11581 acatgcaaaa agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt
    11641 tcttcgttaa agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac
    11701 aagccgtgga agaggcgacc atcaaagcgc cgctgcgtca caatgcggta gaaatttttg
    11761 acgaagtaaa caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggatatcg
    11821 tccccgacgg tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac
    11881 caggccgctc gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct
    11941 tcgaaaatat ctccaccctc gcagtaaacg cctgtccacc ggtactggtg ggcgtgggca
    12001 tcgccacctc ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg
    12061 gcagccgcca ccccaatcca aaagcggcag agctggagct acgcctggaa gaaggactca
    12121 accgtctggg gattggtcca caagggctaa ctggcaacag ttcagtgatg ggcgtgcata
    12181 tcgaatctgc cgcccgccat ccgtcaacca tcggcgttgc tgtttctacc ggttgctggg
    12241 cgcatcgtcg cggcacactg ctggttcatg ccgatctcac ctttgaaaat ctgtctcaca
    12301 cccggagcgc gttatgaaaa agatcctgac aaccccgatc aaagctgaag atctgcaaga
    12361 tattcacgtc ggcgatgtga tctatctgac cggtacgctg gtgacctgcc gtgacgtttg
    12421 tcaccgccga ttgatcgaac tgaaacgtcc gctcccttac gatctcaacg gcaaagcgat
    12481 tttccacgct ggtcccatcg tgcgcaaaaa cggcgacaaa tgggagatgg tctccgtcgg
    12541 tccgacaacc agtatgcgta tggaaagttt tgaacgtgaa tttatcgagc agaccggcgt
    12601 gaaactggtg gtcggcaaag gcggaatggg gccgctgacc gaagaaggct gtcagaaatt
    12661 caaggcgcta catgtaattt tcccggcagg ctgcgcggtg ctggcggcaa cccaggtgga
    12721 agagattgaa gaagtgcact ggacagaact cgggatgccg gagtcactgt gggtctgccg
    12781 ggtcaaagag ttcggcccgc tgattgtctc tattgatacc cacggcaaca acctgatagc
    12841 cgaaaacaaa aagctgttcg ccgaacgccg cgatcccatc gtggaagaga tctgcgagca
    12901 cgtccattac atcaaataac cctcccggag aggctcgccc cctctccttt ttcgcaggca
    12961 taacacgatg aaaccttcca ctgaatggtg gcgatacctt gcgccgctgg cggtcatcgc
    13021 cattattgct ctaattccgg ttcccgcagg gctggagagt catacctggc tctactttgc
    13081 cgtttttact ggcgtaatcg ttggactgat cctcgaaccc gtgccgggtg ccgtggtggc
    13141 gatggtgggt atctccatca tcgccatact ctctccctgg ctgctgttca gcccggagca
    13201 gctcgctcag ccaggcttta aattcactgc aaaatccctc tcgtgggccg tttccggttt
    13261 ttctaattcg gttatctggc tgattttcgc cgcctttatg tttggcacag gctatgaaaa
    13321 aaccgggctg gggcgacgta tcgcgctgat tctggtgaaa aagatggggc atcgcacgct
    13381 gtttcttggc tatgcggtga tgttctccga gctgatcctg gcacctgtca caccgtccaa
    13441 ctcggcgcgt ggtgcgggga ttatctatcc catcatccgt aacctgccgc cgctctatca
    13501 atcacaacca aacgacagca gttcgcgcag tattggctcg tacatcatgt ggatggggat
    13561 tgttgccgac tgcgtgacca gcgccatttt cctgacggcg atggctccta acttgctgtt
    13621 aattggactg atgaaaagcg catctcacgc cacgctgagt tggggcgact ggttcctcgg
    13681 gatgttgccg ctcagtattt tactggtcct gctggttccc tggctggctt acgtgctgta
    13741 cccgcctgta ctgaagtcag gcgatcaggt gccgcgctgg gcagagacgg aactgcaggc
    13801 aatgggcccg ctctgttcgc gtgaaaaacg gatgctgggg ctgatggtag gcgcgctggt
    13861 gctgtggatt ttcggcggtg attatatcga tgccgcgatg gtcggttaca gcgtggtggc
    13921 gctgatgctg cttctgcgca ttatcagttg ggacgatatt gtcagtaata aagcggcgtg
    13981 gaacgttttc ttctggctgg cctcgcttat caccctcgcg accggactca acaacaccgg
    14041 ttttattagc tggtttggca aactgttagc aggcagctta agcggttatt cgccaacgat
    14101 ggtgatggtg gcgttgattg tggtgtttta tctactgcgc tacttttttg ccagcgccac
    14161 ggcgtatacc tccgctctcg caccgatgat gattgccgct gcgctggcga tgccggaaat
    14221 cccgctgccg gtgttctgcc tgatggttgg tgcggcaatt ggtctgggga gcattcttac
    14281 accatacgcc accggcccca gtccgattta ctacggcagt ggttatctgc caacggcgga
    14341 ttactggcga ctgggggcga tttttgggct gatattcctc gtattgctgg tgattaccgg
    14401 cttactgtgg atgcccgtgg tgttgcttta agtaaggacg gtgccggaag taactctccg
    14461 gcacccgata gttttacgca gccggtaact ccgccagcgg ccagcgcgga cgcacgctaa
    14521 cgccgagatc cgccgtcgcg cctgctttaa accgcaccat tccggcatag gcgatcatcg
    14581 cgccgttatc agtacaaaat tccggacgcg cgtagaacac ttcgccgcgg cgttttttca
    14641 tcatttcagc cagcttcgcc cgtaacgtgc ggttagcact cacgccgccc gccatgacca
    14701 gtcgcttaaa gcccgtctga tccagcgcac gcttacactt aatcatcaac gtatcgacca
    14761 ccgcatcttc aaaggcgcgg gcgatgtcag cacgcgtctg gtcgtcggtg ccgttgtcac
    14821 gaatggtatt tgccgcgaag gttttcaggc cggagaagct gaaatccagc cccggacggt
    14881 cggtcatcgg acgcgggaaa acaaagcgcc cggcagtacc ctgcgccgcc attttcgaca
    14941 gtaacggtcc gccaggataa tccagcccca gcagcttcgc ggttttatca aacgcttcac
    15001 cggcggcatc atcgatagac tcgccgagca gctcgtactg accaatgcca gtcacgctga
    15061 ttaactgcgt atgaccgccg gaaaccagca gcgcaacaaa cggaaattcc ggcgggttat
    15121 cttccagcat cggcgctaac agatgccctt ccatatggtg tacagggatc gccggaacgt
    15181 tccaggcaaa cgccagagaa cgccccacgg tcgcgccaac cagcagtgcg ccgactaatc
    15241 cagggcctgc ggtataggcc acagcatcaa tgtcttttgc cgttaaacca gactccttta
    15301 gcgccgcctg gatcaacggt acggttttac gcacatgatc gcgggaggcc agttcaggca
    15361 caacgccccc gtagtcagcg tgcaatttca cctgactata caattggttg gctaacaaac
    15421 ctttttcatc gtcgtaaatg gcgatgccgg tttcatcgca ggaagtttca atacccagta
    15481 cacgcatgac ttgttttacc tcgctttatt accgcgcagt gtaggaccaa tgcgggttga
    15541 tgtaaaactt tgttcgcccc tggagaaagc ctcgtgtata ctcctcaccc ttataaaagt
    15601 ccctttcaaa aaaggccgcg gtgctttaca aagcagcagc aattgcagta aaattccgca
    15661 ccattttgaa ataagctggc gttgatgcca gcggcaaacc gaattaatca aaggtgagag
    15721 gcacatgccg gtaattaaag tacgtgaaaa cgagccgttc gacgtagctc tgcgtcgctt
    15781 caagcgttcc tgcgaaaaag caggtgttct ggcggaagtt cgtcgtcgtg agttctatga
    15841 aaaaccgact accgaacgta agcgcgctaa agcttctgca gtgaaacgtc acgcgaagaa
    15901 actggctcgc gaaaacgcac gccgcactcg tctgtactaa ttccccgaga gcgttgctct
    15961 ccgatcagac cgagttgtag ttgtaaggcc gtgcttccga aaggaatgcg cggcttattt
    16021 tcgtttatga attgctaaaa atcggggcct atggctggac gaatcccacg cgtattcatt
    16081 aatgatctgc tggcacgcac tgacatcgtc gatctgatcg atgcccgtgt gaagctgaaa
    16141 aagcagggca agaatttcca cgcgtgttgt ccattccaca acgagaaaac cccgtccttc
    16201 accgttaacg gtgagaaaca gttttaccac tgctttggat gtggcgcgca cggcaacgcg
    16261 atcgacttcc tgatgaacta cgacaagctt gagttcgtcg aaacggtcga agagctggcg
    16321 gcaatgcaca atcttgaagt gccatttgaa gcaggtagcg gccccagcca gatcgagcgc
    16381 catcagcggc aaacgcttta tcagttgatg gacggtctga atacgtttta ccaacaatct
    16441 ttacaacaac ctgttgccac gtctgcgcgc cagtatctgg aaaaacgcgg attaagccac
    16501 gaggttatcg ctcgctttgc gattggtttt gcgcctcccg gctgggacaa cgtcctgaag
    16561 cggtttggcg gcaatccaga aaatcgccag tcattgattg atgcggggat gttggtcact
    16621 aacgatcagg gacgcagtta cgatcgtttc cgcgagcggg tgatgttccc cattcgcgat
    16681 aaacgcggtc gggtgattgg ttttggcggg cgtgtgctgg gcaacgatac ccccaaatac
    16741 ctgaactcgc cggaaacaga cattttccat aaaggccgcc agctttacgg tctttatgaa
    16801 gcgcagcagg ataacgctga acccaatcgt ctgcttgtgg tcgaaggcta tatggacgtg
    16861 gtggcgctgg cgcaatacgg cattaattac gccgttgcgt cgttaggtac gtcaaccacc
    16921 gccgatcaca tacaactgtt gttccgcgcg accaacaatg tcatttgctg ttatgacggc
    16981 gaccgtgcag gccgtgatgc cgcatggcga gcgctggaaa cggcgctgcc ttacatgaca
    17041 gacggccgtc agctacgctt tatgtttttg cctgatggcg aagaccctga cacgctggta
    17101 cgaaaagaag gtaaagaagc gtttgaagcg cggatggagc aggcgatgcc gctttccgca
    17161 tttctgttta acagcctgat gccgcaagtt gatctgagta cccctgacgg gcgcgcacgt
    17221 ttgagtacgc tggcactgcc attgatatcg caagtgccgg gcgaaacgct gcgaatatat
    17281 cttcgtcagg aattaggcaa caaattaggc atacttgatg acagccagct tgaacgatta
    17341 atgccaaaag cggcagagag cggcgtttct cgccctgttc cgcagctaaa acgcacgacc
    17401 atgcgtatac ttatagggtt gctggtgcaa aatccagaat tagcgacgtt ggtcccgccg
    17461 cttgagaatc tggatgaaaa taagctccct ggacttggct tattcagaga actggtcaac
    17521 acttgtctct cccagccagg tctgaccacc gggcaacttt tagagcacta tcgtggtaca
    17581 aataatgctg ccacccttga aaaactgtcg atgtgggacg atatagcaga taagaatatt
    17641 gctgagcaaa ccttcaccga ctcactcaac catatgtttg attcgctgct tgaactgcgc
    17701 caggaagagt taatcgctcg tgagcgcacg catggtttaa gcaacgaaga acgcctggag
    17761 ctctggacat taaaccagga gctggcgaaa aagtgattta acggcttaag tgccgaagag
    17821 cgatcgggaa gcccccgaca gccgcactga gaggcagcgg caaatatata agtacgccct
    17881 cgtaattatc gttggcggta aacaaccgtt ggatttcagc gttaacggct gaaggacatc
    17941 gggtcaatcg cccaacacca acctcatgaa ataagtgtgg ataccgtctt atggagcaaa
    18001 acccgcagtc acagctgaaa cttcttgtca cccgtggtaa ggagcaaggc tatctgacct
    18061 atgccgaggt caatgaccat ctgccggaag atatcgtcga ttcagatcag atcgaagaca
    18121 tcatccaaat gatcaacgac atgggcattc aggtgatgga agaagcaccg gatgccgatg
    18181 atctgatgct ggctgaaaac accgcggacg aagatgctgc cgaagccgcc gcgcaggtgc
    18241 tttccagcgt ggaatctgaa atcgggcgca cgactgaccc ggtacgcatg tacatgcgtg
    18301 aaatgggcac cgttgaactg ttgacccgcg aaggcgaaat tgacatcgct aagcgtattg
    18361 aagacgggat caaccaggtt caatgctccg ttgctgaata tccggaagcg atcacctatc
    18421 tgctggaaca gtacgatcgt gttgaagcag aagaagcgcg tctgtccgat ctgatcaccg
    18481 gctttgttga cccgaacgca gaagaagatc tggcacctac cgccactcac gtcggttctg
    18541 agctttccca ggaagatctg gacgatgacg aagatgaaga cgaagaagat ggcgatgacg
    18601 acagcgccga tgatgacaac agcatcgacc cggaactggc tcgcgaaaaa tttgcggaac
    18661 tgcgcgctca gtacgttgta acgcgtgaca ccatcaaagc gaaaggtcgc agtcacgctg
    18721 ccgctcagga agagatcctg aaactgtctg aagtattcaa acagttccgc ctggtgccga
    18781 agcagtttga ctacctggtc aacagtatgc gcgtcatgat ggaccgcgtt cgtacgcaag
    18841 aacgtctgat catgaagctc tgcgttgagc agtgcaaaat gccgaagaaa aacttcatta
    18901 ctctgtttac cggcaacgaa accagcgata cctggttcaa cgcggcaatt gcgatgaaca
    18961 agccgtggtc ggaaaaactg cacgatgtct ctgaagaagt gcatcgcgcc ctgcagaaac
    19021 tgcagcagat tgaagaagaa accggcctga ccatcgagca ggttaaagat atcaaccgtc
    19081 gtatgtccat cggtgaagcg aaagcccgcc gtgcgaagaa agagatggtt gaagcgaact
    19141 tacgtctggt tatttctatc gccaagaaat acaccaaccg tggcttgcag ttccttgacc
    19201 tgattcagga aggcaacatc ggtctgatga aagcggttga taaattcgaa taccgccgtg
    19261 gttacaagtt ctccacctac gcaacctggt ggatccgtca ggcgatcacc cgctctatcg
    19321 cggatcaggc gcgcaccatc cgtattccgg tgcatatgat tgagactatc aacaaactca
    19381 accgtatttc tcgccagatg ctgcaagaga tgggccgtga accgacgccg gaagaactgg
    19441 ctgaacgtat gctgatgccg gaagacaaga tccgcaaagt gctgaagatc gccaaagagc
    19501 caatctccat ggaaacgccg atcggtgatg atgaagattc gcatctgggg gatttcatcg
    19561 aggataccac cctcgagctg ccgctggatt ctgcgaccac cgaaagcctg cgtgcggcaa
    19621 cacacgacgt gctggctggc ctgaccgcgc gtgaagcgaa agttctgcgt atgcgtttcg
    19681 gtatcgatat gaacaccgat cacacgctgg aagaagtggg taaacagttc gacgttaccc
    19741 gtgaacgtat ccgtcagatc gaagcgaagg cgctgcgcaa actgcgtcac ccgagccgtt
    19801 ctgaagtgct gcgtagcttc ctggacgatt aatcggtagg ccggatcagg cgttacgccg
    19861 cacccggcac ctgtcccttt gcacaaacgc caccttttcg gtggcgtttt ttatcgccca
    19921 cgcaccacca gcgcctggtc cagctcgcga tacgcttcaa ccagtttctc cagtgaaacg
    19981 cgacttaaac cgctgggatt tggcagcacc caaatctgcg tcgaaccaat gctgagcgtt
    20041 tgtttccccc actgtgcacc acgctggctg aatccctgtt catatgcttg tttgcccaga
    20101 atcgccaacg cttgcggctg ataatcttca attttttcaa tcagcttacg cccacctgcg
    20161 tgtagctctt gctttgaaat ttcattggct tgcaccgttg gcctgtctac cagtttggtg
    20221 acgccacaac gataatccag cagatgctgt gcctcctgcg gcttcaactg atggtcggta
    20281 aacccggcct gatatatcac cttccagaag cgatttgccg gatgagcaaa gggaaaacca
    20341 gtcccggcgg atgaaagccc agggttgata ccgcaaaaca cgacccgtaa ccctggagcc
    20401 aaaatatcct caaccatatt tactcctttc taacatcacc aggaaaagta taacggattg
    20461 aaaatacatt gtttataaaa acagcaggcg cgcggtaatg gctggattgc gacacggagt
    20521 tactttataa tccgct
//
LOCUS       DBFIFM010000070        20256 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.070, whole genome shotgun sequence.
ACCESSION   DBFIFM010000070 DBFIFM010000000
VERSION     DBFIFM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.070"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..795
                     /locus_tag="N7C74_002230"
     CDS             <1..795
                     /locus_tag="N7C74_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2432093.1"
                     /translation="LPIKVQNGGLFISRGVGRHSARRLESWEIIFVEKGRLTIQEEER
                     IFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLSVPQQTKV
                     VDPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAGTTLAWKA
                     QKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVREAEKLLIS
                     EARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            797..937
                     /locus_tag="N7C74_002231"
                     /pseudo
     CDS             797..937
                     /locus_tag="N7C74_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
     gene            1039..1512
                     /gene="ysaA"
                     /locus_tag="N7C74_002232"
     CDS             1039..1512
                     /gene="ysaA"
                     /locus_tag="N7C74_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGU2432094.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(1664..2917)
                     /gene="avtA"
                     /locus_tag="N7C74_002233"
     CDS             complement(1664..2917)
                     /gene="avtA"
                     /locus_tag="N7C74_002233"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HGU2432095.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(3095..5125)
                     /gene="malS"
                     /locus_tag="N7C74_002234"
     CDS             complement(3095..5125)
                     /gene="malS"
                     /locus_tag="N7C74_002234"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGU2432096.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKAIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            5445..6269
                     /gene="bax"
                     /locus_tag="N7C74_002235"
     CDS             5445..6269
                     /gene="bax"
                     /locus_tag="N7C74_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HGU2432097.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTSRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(6377..7555)
                     /gene="xylR"
                     /locus_tag="N7C74_002236"
     CDS             complement(6377..7555)
                     /gene="xylR"
                     /locus_tag="N7C74_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HGU2432098.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(7633..8814)
                     /gene="xylH"
                     /locus_tag="N7C74_002237"
     CDS             complement(7633..8814)
                     /gene="xylH"
                     /locus_tag="N7C74_002237"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HGU2432099.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(8792..10333)
                     /gene="xylG"
                     /locus_tag="N7C74_002238"
     CDS             complement(8792..10333)
                     /gene="xylG"
                     /locus_tag="N7C74_002238"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HGU2432100.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(10411..11403)
                     /gene="xylF"
                     /locus_tag="N7C74_002239"
     CDS             complement(10411..11403)
                     /gene="xylF"
                     /locus_tag="N7C74_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU2432101.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLQPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            11769..13091
                     /gene="xylA"
                     /locus_tag="N7C74_002240"
     CDS             11769..13091
                     /gene="xylA"
                     /locus_tag="N7C74_002240"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HGU2432102.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAACMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            13163..14617
                     /gene="xylB"
                     /locus_tag="N7C74_002241"
     CDS             13163..14617
                     /gene="xylB"
                     /locus_tag="N7C74_002241"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HGU2432103.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECALLEARVPLSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            14786..15127
                     /gene="yiaB"
                     /locus_tag="N7C74_002242"
     CDS             14786..15127
                     /gene="yiaB"
                     /locus_tag="N7C74_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HGU2432104.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            15173..15610
                     /gene="yiaA"
                     /locus_tag="N7C74_002243"
     CDS             15173..15610
                     /gene="yiaA"
                     /locus_tag="N7C74_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HGU2432105.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(15652..16647)
                     /gene="wecH"
                     /locus_tag="N7C74_002244"
     CDS             complement(15652..16647)
                     /gene="wecH"
                     /locus_tag="N7C74_002244"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HGU2432106.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRALGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYPYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            16822..17121
                     /gene="ysaB"
                     /locus_tag="N7C74_002245"
     CDS             16822..17121
                     /gene="ysaB"
                     /locus_tag="N7C74_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HGU2432107.1"
                     /translation="MMMNSFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            17216..18127
                     /gene="glyQ"
                     /locus_tag="N7C74_002246"
     CDS             17216..18127
                     /gene="glyQ"
                     /locus_tag="N7C74_002246"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HGU2432108.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            18137..20206
                     /gene="glyS"
                     /locus_tag="N7C74_002247"
     CDS             18137..20206
                     /gene="glyS"
                     /locus_tag="N7C74_002247"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HGU2432109.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
BASE COUNT         5021 a         4949 c         5198 g         5088 t
ORIGIN      
        1 ttaccgatta aagtgcaaaa tggtggatta tttatttccc gtggcgtagg gaggcattct
       61 gcaaggcggc tggagtcatg ggaaattatc tttgtcgaaa aaggtcgatt aacgattcag
      121 gaagaagaac ggatatttct ggtcgatgcg ggagagagtt tattactctg gccgaaccga
      181 caacatgttg gggtggagga ttttcccgcc gatctcaaat tttactggct gcattttgag
      241 gttaaagcgc cggacaacga ccctcgctgg cttactcatc ttagcgtgcc gcaacaaacg
      301 aaggttgtcg atccgcaggc gctggtggcc ctgtttcgcc agttcttaag cgaacaggaa
      361 aagcatcaac gcactccggc gctggagttt attgtgctgc tcattttgca gcagctcacc
      421 cgtgacgcgc gtcttgatga acaggccgat ggcgcaggca ccacccttgc ctggaaagcg
      481 cagaaactga tccgcacgca atatcatcgc ccgctttcta cggcgcttct ggcccgcgag
      541 ctgtactgca atgccgatta tcttgggcgc gtctaccggc gggtgtttca tctgacgtta
      601 acagaagcaa tccatcgcca gcgggtgcgc gaagccgaaa agctgttaat tagcgaagcg
      661 cgatcgctca cggaagtggc ggcgttgtgc gggtttaatg acgttggcta tttccggcag
      721 atttttcgca aacacaccgg tttaacgcca gcagcgtgga agcggcgtta cagcaaggaa
      781 catatcaatt cgtagtgccg gggcgatttc gctttcgaca tcacgtctga aacaggtggg
      841 ggagaaaaat ctcctgaaac cactgcgtga aaccgcgcag gctatttcta atgaactggg
      901 atttactgtc agggacgacc agggcgcgat cacataacgc tttggacaag tgccaaaact
      961 ttaacatttc cttcgttgga tcaaagcagt agggacgcgc tctctggcac tctgctgttt
     1021 tagtgcaaag gagtgatcat gaaccggttt attattgcgg atgcgacgaa atgtatcggt
     1081 tgccgtacct gtgaagtggc ttgcgcagtg tcgcatcagg agaatcagga ttgcgctgcg
     1141 ttgtcaccag acgagtttat ttcccgtatt cgtgtcatta aagaccacag ctggaccacg
     1201 gcagtagcct gtcatcagtg tgaagatgca ccgtgcgcga atgtctgccc tgttgacgcg
     1261 ataagccgcg aacatgggca tattttcgtt gaacaaacac gttgcattgg ctgtaaaagc
     1321 tgtatgctgg cttgcccgtt tggtgcgatg gaggtcgttt cttcgcgcaa aaaggcgagg
     1381 gcgattaagt gcgatctgtg ctggcatcgg gagacgggac cggcctgtgt tgaagcctgc
     1441 ccgacaaagg cgttgcagtg catggatgta gagaaagtgc agcggcatcg gctacggcag
     1501 cagcctgttt gaaacgtttt tccggcgttt ctcaacgccg gaatagtgcg ctttgtttat
     1561 gccagatgcg gcgtaaacgc cttatccggc ccacaaattc ttgcaaatta aatatattgc
     1621 agaaatcatg taggcctgat aagcgtagcg catcaggcaa tttttagtga ctttcagccc
     1681 aggctctttc tatctcttcc gccagaatct tcacccccgc ctcaattttc tccggctctg
     1741 gtacgtagtt catgcgcata cattgatgcg tatgcggcca cggtttgtcc agccctggga
     1801 agaagttgtg ccccggcacc atcagcacgc cgcgtgcttt caggcgctga tagagctgct
     1861 cggtcgtaat gggcaaatcc ttaaaccaaa gccagaggaa aatggctcct tctggtttat
     1921 gaattaggca gcgatcttcc ggtaaatagc ggcgaatgat ggcgatagtt tcctgaacac
     1981 gctggtagta aaacggtttg atgactgttt cagacaggcg cagcagatcg ttacgcttaa
     2041 tcatttcaca catcatcgcc ggaccaatac cgccaggtgc caggctgata atgccgttca
     2101 tattggtgat ggcggtgatg attttttcat tggcgatgat aatgccgcag cgggagccag
     2161 gcagccccag cttggaaaga ctcatgcaca gcacgatatt cggattccat agcgggcgcg
     2221 cttcactgaa gatgataccc gggaacggga cgccataagc gttatcaatc accagcggaa
     2281 tgccgtgttg attcgccagc gcgtcaagct tcagcaactc ttcgtcagta atcacattgc
     2341 ctgttggatt cgtcggccgg gagacgcaaa tcatcccggt ttcttcgcca atatgcagat
     2401 gctcaaaatc gacgtggtat ttaaactggc cttccggcag cagttcaata ttcggacgcg
     2461 cagagacaaa cagatcttct tccagtccgg cgtcagcgta gccaatgtat tccggtgcca
     2521 gcgggaacag cacttttttg acccgaccat cggcacggcg tccggcaaac agattaaata
     2581 agtagaaaaa cgcgctctgg ctgccgtttg ttagtgcaat attctgtgct tcgatatccc
     2641 aacccaactt ctcgcgcagc attccggcaa gcagtgtgag tagctccgtt ttcccctgtg
     2701 gaccgtcgta attgcacagt gcatcagtcg ctttgccact ttccagcatg tcggtcagta
     2761 gcgtctggaa gtagtcctgc atttccggga tctgcgccgg attaccgccg ccgagcataa
     2821 tcgcgccagg cgtgcgtaaa ccgtcgttca gatcttccat cagcagcgta atgccggagt
     2881 ggcgggtaaa tttgtcacca aaaagggaga atgtcatagc gggatatctg tcgaactctg
     2941 tagcaaggaa ggtaacaata acgctacatt tgctggggtg caaatcggtc tgttgaagag
     3001 tgagcggtgc tttatcctgc aacgctgatt agggctgaca ttttatcctg gttggcggct
     3061 tcccgaggta ctgacgaacg ggaagccgga aaagtcactg ttgcccagcc cagatgacca
     3121 gcactttatc gtcgccatgc tcacgaacaa agccgtagcc ctgcttcagc gaaagtgtcg
     3181 tttgtttgcc cgcgccaatt gcgggatggc gggcgcggaa ctggctgatt ttctgccagt
     3241 gcgcgacgtt ggcggcagat ttaccgctaa catcctgcca gttcatatcc gaacgtgtac
     3301 cttgcagcgg atcagaacct gtaggaccga acggacgcga ggattcatcg ccataaaaga
     3361 tttgtaccgc gcctggcgct aatagtaata actctgctgc tttgtcgccc ccttcacgga
     3421 acagacgggt atcatgcgac gagaggtagc tcaacacgtt gaaaccctgc aatttctccg
     3481 ccatttgctg ccaggtcgta tccatctgcg caatacaatc gacagctttc gccgcctgct
     3541 cctgataatc gaaattgatc atcgcatcga agccgtggcg atagtagtca ctttgcatca
     3601 cgccgtggcc ccaggcttca ccggtcatcc agaaaggttt gtcatctaat gctttgtcgg
     3661 ggttagcttt tttccattcg cgaagcgcgg cgctggcttc ggttttcagt tgctgccaag
     3721 cgggcaactc aacatgtttg gcggtatcga cccgaaaacc atcaatccca tagtcgcgga
     3781 cccactgact taaccagtgg gttaagtaat cgcgaggggt aaagccgtcg atggctttag
     3841 cgtgggtatc cgttttgttt ttatagaaca ccggcagacc agaagcggta gttgattcgg
     3901 ttttgatatc cggcaaaaag gctagcgaca tggtgagatc gtcgaatcca ggattgtcgt
     3961 aatcgccgat atcggtacgg atccagtttt ttccccacca tttatcccag cctgttttgt
     4021 cgctgaaatt aatgtaatcg ttaaagctat gccaggtttg cccggcggca ggtttccagt
     4081 cgctccagcg ttcacccagc gtttttttca cttcgtcacc agaaagatat aacgcgccaa
     4141 actgatactc ctgcatatcc gccagcgtgg catagccggt gtggttcatc acgacatcaa
     4201 agagaatacg aataccgcgc tgatgtgcgc tatcaaccag cgtccgtaga tcggcttcgt
     4261 tgcccatatt ggcatcaaga ttcgtccagt cctgtgtgta ataaccgtgg taggcataat
     4321 gcgggaaatc gccttttgta ccgccgccga cccagccgtg aatttgctca aatggggcgc
     4381 ttatccataa agcattaacg cccaactgct ggaggtaatc cagtttgttg gtcaggccgc
     4441 gtaaatcgcc gccgtgaaaa gtgccaattt ccgccatacc gtctttatga cgtccgtaac
     4501 tctggtcatt actgggatcg ccgttttcga aacgatctgt cagcacaaag taaaccgtgg
     4561 cgttatgcca gtcgaaaggg gcagaggcgt cagtttctgc ccgttccagc aggagtaaac
     4621 cgttgctggt agcagcgggt tgtaacgtta tttgaccgtt cttcactatc gcaatttgct
     4681 ggctgtaata atcccgtacg gcggctcctt ccgggaaagt ggcgctgaca tccagcgtga
     4741 gcggtaatcc atcccatttc gggcattcac ggaccaggtt tgctaccggt tcggcggcgt
     4801 tctggatgga aatcatcaat gttggcgtac cggagcgggt gtctatttgc agcgtatatt
     4861 tgccgtccct gaacaatcgc cattgaggcg gcgtgttgct acaaggttgc agggaaagca
     4921 tctgattgag ttttatcgca tctgcaggct gccagcactg ctggtcaaaa tttagcgtga
     4981 gtggacgcgt acctttgggc aactgcgcgt ggctgacaaa tgttcccgtt ccctgttcgc
     5041 taaaggcagg gaaccccgga gaagtccagc tggcggcaac ggcgaagcca ggaaggagtg
     5101 tcagaaaaca ggcggcgagt ttcatggtgg atgagtcctt attgctgcga tttcagacag
     5161 tttgccagcg ataagccaga caaaactcat ccctgagcgg taagtccaca ggatgagaag
     5221 caagggtgag cgatcgcgcg caaaaaacgg ctgaattttg cgataaaccc cacattttct
     5281 gcgatttagc gccaatctga atcgttaact cgtgacatag tttcagattt ggactattcc
     5341 ttggggtgct cttgacagct atttttgata tgatttgaga ttcaactctc aaatttgtga
     5401 aaaatataag gtgttggaat gattaaatcc gaccaggaga cctgatgatt ttgactccca
     5461 tacgacgata tggggcgatg attcttatgt tactcactct ggtgttttcg agtgaggtgt
     5521 tagcgaagac gcacacaaca acagcgagtc aaaagtccca cttaactaaa gctagtaata
     5581 aacaggtaag cagtaaacaa gagtattctc gcaatagtgc aaagagtagt tcacttcctg
     5641 atttgcgaaa atacccttcc ggaacatcaa ggaaaaaggc gtttctccgg accgtaatgc
     5701 cttacattac cagccaaaat gcagccatta ctgcagaacg taactggctg atttcaaaac
     5761 agtatcaggg ccaatggtca cctgctgagc gtgcgcgtct gaaagacatc gccaaacgct
     5821 acaaggtgaa gtggtctggt aatacgcgaa aaatcccgtg gaataccttg cttgaacgcg
     5881 tagacattat ccctaccagt atggtggcga cgatggctgc agcagaaagc ggttggggaa
     5941 cgtcgaagct ggcgcgcaac aacaacaatc ttttcggcat gaaatgcatg aaaggacgtt
     6001 gtaccaatgc gccaggtaaa gtgaaagggt actcacagtt tagttctgtc aaagaatcgg
     6061 tgagcgccta tgtcactaac ctcaatacgc atccggctta ctcttcgttc cgtaaatcgc
     6121 gtgcgcagct gcgtaaagct gatcaggaag tgactgccac agcgatgatt cacaagctga
     6181 agggctactc gaccaaaggg aagagttata acaactacct gttcgcaatg taccaggata
     6241 accaacggtt aatcgcggcg catatgtaat tgtatttcct cgagccttat ccgacttgtc
     6301 ggtcggataa ggcttttgat cgcatcaggt ggttgtgctg agttacctga tgcgacgccg
     6361 acaattctca tcatcgctac aacatgacct cgctatttac atcgcgatac tcttttggcg
     6421 tcgtgtcata tgctttttta aaaacagagt agaaatattg cagcgatgga taaccgcaca
     6481 tttgcgatat ctcattgatc gacaaggtgg ttgaaatcag cagactgcgc gctttctcca
     6541 gcttctcagc atgaatcatg gcatggatgg tttcacccac ctcttcttta aaacgcttct
     6601 caagattgga gcgcgagatc ccgaccgcat cgagtacctg atccacttta atccctttac
     6661 aggcgtgatt acgaatgtaa tgcatggcct gaataacggc gggatcggtc agcgaacggt
     6721 aatctgttga gcgccgttca atgacgcgaa ctggagggac caaaatccgc tgtagcggca
     6781 tttcttcttt atctaataat cgatgcaaca gttttgccgc ctgatagccc atttgccgcg
     6841 cgccctgagc gaccgaagaa agggcgacac gcgacagata gcgggtcagt tcttcgttat
     6901 cgatgccaat cacgcataat ttttccggta cgggaatgtg tagatgttca catacttgca
     6961 gaatatgccg cgcccgggcg tcagtaacgg caataatccc ggtttgcggt ggcagtgttt
     7021 gtagccagtc tgccagccga ttttgcgcgt gttgccagtt ctctggtgcg gtttctaacc
     7081 cctgataaac cactccgcga tacttttctt cggcgacaag ctgacgaaat gcatattcgc
     7141 gttcagtggc ccaacgtttg ccgcttgatt ccggaagacc ataaaaagca aagcggttaa
     7201 cgcctttctc ttttaaatgc aaaaatgcgc tttcaaccag cgcatagtta tcggtggcaa
     7261 tgtaatgaac gggtgggtaa ctttctgcaa ggtgatacga gccgccaacc ccaacaatgg
     7321 ggacgtcgac atcagccagc gcttgctcga tctgtttgtc gtcgaagtcg gcaatgacgc
     7381 catctcctaa ccagtccttg attttatcaa tgcgggcgcg gaaatcttct tcaatgaaaa
     7441 tatcccattc cgattgtgac gcctgtaaat attcccctac gccttctact acctgccggt
     7501 cataggcttt attggcattg aacagtaatg tgatgcggtg acgtttagta aacatggttc
     7561 ttttcctgct gaatcatgca aaaactcaaa accggtaata cgttaccggc tttgagaaaa
     7621 tttttatcaa aatcaagaac ggcgtttggt tgcggagtcc atccatactg ccagcaacag
     7681 aatcgcacct ttaacgatat actgccagaa ggtcggtaca tccatcatac tcataccgtt
     7741 atccagtgaa gccatgatga atgcccccat taccgctcca gccacgctcc cgacgccgcc
     7801 tgccagactg gtgccgccaa tcacgcatgc tgcaattgcg tccagctcgg cgatattccc
     7861 cgcagaaggt gaaccagcgc caagtcgaga actgaggatt aatccggcga tggctaccat
     7921 taatccgtta atagcgaaca cggcaagttt ggtgcgttca acgttaattc cggagagacg
     7981 tgctgcttcc agattgccgc cgatggcata aatgcgtcgt ccaaatgctg tccgcgttgc
     8041 cataaacatt ccgccgagta acagcaacgt cagcagcaga acaggagtag gaacgccacg
     8101 gtaatcattc aacagccaga ttgcgcctaa tacgatgata gcggttaaag cctggcgacc
     8161 gactactgcg gtagaggctg gagactgcaa acccaaagcc tgacggcgca ttcttccgcg
     8221 ccattgccag ccaacaaaag ccattaagcc aagcgcgcca atgatgaagc cggtgctggc
     8281 ggggagatag ctttgcccaa tttgtgacat cgcggcgctg gtgggggata cagtcgtgcc
     8341 gttggtgatg ccaatgagta tgccgcgaaa tgccaacatg cccgcgagag tgacaataaa
     8401 tgaagggact ttgcggtacg cgacccacca tccgttccag gcaccgagaa gcagtcccag
     8461 aaccaacgtc acaatgatgg taagtggcaa aggccagcct agccagacgt cacaaatcgc
     8521 cgcgacgcca cctaatagcc ccatcattga gccgacggaa aggtcgattt cagcagaaat
     8581 tatgacgaac accattccta ccgcgaggat gccggtaatc gcggtctggc gtaacaggtt
     8641 ggagacgtta cgggcgctta agtaggcacc atcggtggtc caggtaaaga acagcatgat
     8701 tgcgatgata gctgcaatca tcacgaagac ctgcaaattc agtgatttca gcccggagaa
     8761 gccaccggat gtcggtacgg ccaatttcac ttcagacgga ttgcttttcg acatgatgtt
     8821 cgctcctcaa tgcggcttcc atcacctgct cctgagtcag gttatgattt atcaggttgg
     8881 cttttagttt cccttcatgc attactagta cacgatcgct aaggccgagc acttcaggta
     8941 attcagaaga gatgacaata acggcaatac cctgctggac gagttggtta attaatttgt
     9001 agatctcgta tttcgcgcca atatcgatac ccctggtggg ttcatcaaga atgagaatgc
     9061 gcgggttaag taacagacag cgagcgagga tcgctttttg ctgattgccg ccgctcaaac
     9121 gtccaatagc aaggtcgggg gacgacgttt taactttgag ttgctggatt gattccagaa
     9181 tacatttttg ctctgccgcg tcatcaagct ggctaatgcc accggtaaat ttattgagtg
     9241 cggcgagggt aatattttta ccaaccgcca ttaccggaac gatgccgtcg cgctttctgt
     9301 cttcggggac catcgcaatc ccctgggcga tggcttgctg acagttacga atatctacct
     9361 gtttgccatc aatataaatt tttccttccc attgtccggg ccacacacca aacaggcact
     9421 gaatggtctc ggtacgtccg gcaccaacga gtccggcaat acccagtatt tcgccacgtt
     9481 tcagggaaaa cgagacatca ttaactcgtt taatatgacg attaaccgga tgccatgccg
     9541 tcagatgttc aatacgtaat atttcatctc cggtggtatg tggttcatta gggtaaagcg
     9601 cggttaactc tcgcccgacc atcatggtga taatatcgtc ttcactcatt ccggcagcat
     9661 cacgcgtacc aatgtgctgt ccgtcgcgaa taacgcaaat cgtatcggaa atcgctttga
     9721 cttcgttgag tttgtgcgaa atataaatac aggcgatacc gtgctgttgt agatcgcgaa
     9781 taatatccag taaaaccgac gtttcctgct cagttaatga ggctgtcggt tcatcgagaa
     9841 ttaacaagcg cacctgttta ttaagtgcct tggcaatttc aaccagttgt tgttgcccaa
     9901 gccctaaatc gccaacgcgg gtatcaggtg aaatggataa actgacctgt gcgagcagct
     9961 tctgacagcg tagcgtcatc aggtcataat ccataatgcc attgtgggtt atttcgttac
    10021 ccaggaagat attttccagc acggtcaatt ctttcaccag ggccaattcc tggtgaatga
    10081 tggcgatacc tttgcgttcg gtatcgcgga tgtgactcgc ctgaatctct tctcccgcaa
    10141 aaataatttc gccttcgtag gagccatggg gataaatacc acacagcact ttcatcagcg
    10201 ttgatttacc agacccattt tccccacaga gtgagacgat ttcgccagca ttcaaccgca
    10261 agctgacgtt atcaatcgcc ttcacactgc cgaaggtttt ggtaatgttc ttcatttcaa
    10321 gtagataagg catagcgact ccacctaagc caattcattc acgcggcatg gagagaaatc
    10381 acgcccccgc tccgcgctgg ggcgtaacgc ttacagctcg ctctctttgt ggaatccgtc
    10441 tttaattacc gtatctttga tgttgttttt attcacatcg atcggtgtca ggaggcggga
    10501 ggggacatct ttcaggccat tatttagtga agtatctgct tttggctcct gaccattgcc
    10561 caattcaacg gcaatttctg cggcagtatt tgccagcaat gtaataggtt tatataccgt
    10621 catagtttgc gtaccggcag caatacgttt aatacctgcg agatccgcat cctggccaga
    10681 aattgctact ttccctgata aaccttgcgc gcttaatgcc tgaattgccc cacctgcggt
    10741 ggcatcgttt gaggcaacta cagcatcaat tttgttatta ttggcggtta gcgcgttttc
    10801 cataattttc aatgcgtttt ccggtaacca gccatcaacc cattggtcac caacgacttt
    10861 aatttttccg gaatcaacgt aaggttgtaa cactttcatt tgtccggcgc ggaacagctt
    10921 agcgttgtta tctaccggcg agccgcccat caggaagtaa ttaccttgcg gaacaatatc
    10981 gaccagggct tttgcctgca gttcaccgac tttttcgtta tcgaaagaaa tataaaaatc
    11041 gatatccgca tcgttaatca tacggtcgta agctaatact ttaatgcctt cttgtttggc
    11101 ttcttttaca acgttactta atacctgacc gttatacgga ataatgacaa gaacatcgac
    11161 accccggttt atcatgtttt caatctgcga catttgtgtt tcttcattgc catttgcaga
    11221 ctgtacaaat actttcgcgc cgagagattc tgcctttttc acaaagatat ctcgatcttt
    11281 ttgccagcgt tcaagacgga gatcatcaat cgccatacct attttgactt ctttggcgtg
    11341 tgcagcaacg ttggtaagca ggagtgaggt gcaaagggtg agtagaatgt tctttatttt
    11401 catggtgtag ggccttctgt agttagagga cagctttaat aagtaacaat caccgcgata
    11461 aacgtaacca atttttagca actaaacagg ggaaaacaat tacagatttt tatctttcga
    11521 ttacgatttt tggtttattt cttgatttat gaccgagatc ttacttttgt tgcgcaattg
    11581 tacttattgc atttttctct tcgaggaatt acccagtttc atcattccat tttattttgc
    11641 gagcgagcgc acacttgtga attatctcaa tagcagtgtg aaataacata attgagcaac
    11701 tgaaagggag tgcccaatat tacgacatca tccatcaccc gcggcattac ctgattatgg
    11761 agttcaatat gcaagcctat tttgaccagc tcgatcgcgt tcgttatgaa ggctcaaaat
    11821 cctcaaaccc gttagcattc cgtcactaca atcccgacga actggtgttg ggcaagcgta
    11881 tggaagagca cttgcgattt gccgcctgct actggcacac cttctgctgg aacggggcgg
    11941 atatgtttgg tgtgggggcg tttaatcgtc cgtggcagca gcctggtgag gcactggcgt
    12001 tggcgaagcg taaagcagat gtcgcatttg agtttttcca caagttacat gtgccatttt
    12061 attgcttcca cgatgtggat gtttctcctg agggcgcgtc gttaaaagag tacatcaata
    12121 attttgcgca aatggttgat gttctggcag gcaagcaaga agagagcggc gtgaagctgc
    12181 tgtggggaac cgctaactgc tttacaaacc ctcgctatgg cgcgggtgcg gcgacgaacc
    12241 cagatccaga agtcttcagc tgggcggcaa cgcaagttgt tacagcgatg gaagcaaccc
    12301 ataaattggg tggtgaaaac tatgtcctgt ggggcggtcg tgaaggttac gaaacgctgt
    12361 taaataccga tttgcgtcag gagcgtgaac aactgggccg ctttatgcaa atggtggttg
    12421 agcataaaca taaaatcggc ttccagggta cgttgcttat cgaaccgaaa ccgcaagaac
    12481 cgactaaaca tcaatatgat tacgatgctg cgacagtcta tggcttcctg aaacagtttg
    12541 gtctggaaaa agagattaaa ctgaacattg aagctaacca cgcgacgctg gcaggtcact
    12601 ctttccatca tgaaatagcc accgccattg cgcttggcct gttcggttct gtcgacgcca
    12661 accgtggcga tgcgcaactg ggctgggaca ccgaccagtt cccgaacagt gtggaagaga
    12721 atgcgctggt gatgtatgaa attctcaaag caggcggttt caccaccggt ggtctgaact
    12781 tcgatgccaa agtacgtcgt caaagtactg ataaatatga tctgttttac ggtcatatcg
    12841 gcgcgatgga tacgatggca ctggcgctga aaattgcagc gtgcatgatt gaagatggcg
    12901 agctggataa acgcatcgcg cagcgttatt ccggctggaa tagcgaattg ggccagcaaa
    12961 tcctgaaagg ccaaatgtca ctggcagatt tagccaaata tgctcaggaa cataatttgt
    13021 ctccggtgca tcagagtggt cgccaggagc aactggaaaa tctggtaaat cattatctgt
    13081 tcgacaaata acggctaact gtgcagtccg ttggcccggt tatcggtagc gataccgggc
    13141 atttttttaa ggaacgatcg atatgtatat cgggatagat cttggcacct cgggcgtaaa
    13201 agttattttg ctcaacgagc agggtgaggt ggttgcttcg caaacggaaa agctgaccgt
    13261 ttcgcgcccg catccactct ggtcggaaca agacccggaa cagtggtggc aggcaactga
    13321 tcgcgcaatg aaagctctgg gcgatcagca ttctctgcag gacgttaaag cattgggtat
    13381 tgccggccag atgcacggag caaccttact ggatgctcaa caacgggtat tgcgccctgc
    13441 cattttgtgg aacgacgggc gctgtgcgca agagtgcgct ttgctggaag cgcgagttcc
    13501 gctatcgcgg gtgattaccg gcaacctgat gatgcccgga tttactgcgc ctaaattgct
    13561 atgggttcag cggcatgagc cggagatatt ccgtcaaatc gacaaagtat tattaccgaa
    13621 agattacttg cgtctgcgta tgacggggga gtttgccagc gatatgtctg acgcagctgg
    13681 caccatgtgg ctggatgtcg caaagcgtga ctggagtgac gtcatgctgc aggcttgcga
    13741 cttatctcgt gaccagatgc ccgcattata cgaaggcagc gaaattactg gtgctttgtt
    13801 acctgaagtt gcgaaagcgt ggggtatggc gacggtgcca gttgtcgcag gcggtggcga
    13861 caatgcagct ggtgcagttg gtgtgggaat ggttgatgct aatcaggcaa tgttatcgct
    13921 ggggacgtcg ggggtctatt ttgctgtcag cgaagggttc ttaagcaagc cagaaagcgc
    13981 cgtacacagc ttttgccatg cgctaccgca gcgttggcat ttaatgtctg taatgctgag
    14041 tgcagcgtcg tgtctggatt gggccgcgaa attaaccggc ctgagcaatg tcccagcttt
    14101 aatcgctgca gctcaacagg ctgatgaaag tgccgagcca gtttggtttc tgccttatct
    14161 ttccggcgag cgtacaccac acaataatcc ccaggcgaaa ggggttttct ttggtttgac
    14221 tcatcaacat ggccccaatg aactggcgcg agcagtgctg gaaggcgtgg gttatgcgct
    14281 ggcagatggc atggatgtcg tgcatgcctg cggcattaaa ccgcaaagtg ttacgttgat
    14341 tggtggcggg gcgcgtagtg agtactggcg tcagatgctg gcggatatca gcggtcagca
    14401 gctcgattac cgtacggggg gggatgtggg gccagcactg ggcgcagcaa ggctggcgca
    14461 gattgcggcg aatccagaga aatcgctcat tgagttgttg ccgcaactac cgttagaaca
    14521 gtcgcatcta ccagatgcgc agcgttatgc cgcttatcag ccacgacgtg aaacattccg
    14581 tcgcctctat cagcaacttc tgccattaat ggcgtaaacg ttatcccctg cctgaccggg
    14641 tgggggataa ttcacatcta tatatctcag caattaatta atatttagta tgaatttatt
    14701 ctgaaaatca tttgttaatg gcatttttca gttttgtctt tcgttggtta ctcgtaatgt
    14761 atcgctggta gatatggaga tcgttatgaa aacctcaaaa actgtggcaa aactattatt
    14821 tgttgtcggg gcgctggttt atctggttgg gctatggatc tcatgcccat tgttgagtgg
    14881 aaaaggctat tttcttggcg tgttaatgac agcaactttt ggcaactatg catatcttcg
    14941 cgcagaaaaa ctcgggcaac tggataattt ttttacccat atttgccagt tagttgcgtt
    15001 aatcactatc ggtctcttgt ttatcggtgt tttaaatgca cctatcaatg cttatgaaat
    15061 ggtgatctac cccatcgcct tttttgtctg cttgtttggt caaatgcgtt tgtttcgctc
    15121 ggtatgagca acataaagct cttacacatt caggaatgaa aggaatactg tgatggacaa
    15181 caaaatatca acctattcac cggcctttag tattgtgtca tggatagctc tcgttggtgg
    15241 tatcgttacc tatctgttag ggctatggaa tgcagagatg cagttaaatg aaaaaggata
    15301 ttattttgcc gtactggtat taggactgtt ttctgcggcg tcttatcaaa agaccgttcg
    15361 ggacaagtat gaaggcatac cgaccacttc catttattat atgacctgcc tgactgtctt
    15421 tattatctct gttgcattac tgatggtagg tctgtggaat gcgacattat tactcagcga
    15481 aaaaggtttt tatggactgg ctttcttctt aagcttgttt ggtgcagtag cggtgcagaa
    15541 aaatattcgt gatgccggaa taaacccacc aaaagaaaca caggttaccc aggaagaata
    15601 cagcgaataa ctcacgtaag cccggtcagt ccaatgtgac cgggctttta cttaactcac
    15661 taatctgttt ctgtcgattc gttgtaccag catagaaagt aacaaactcg ctgccaacgt
    15721 cgcgcaaaaa atccaaataa tatccagtat tggccaattt ttaagctcaa ttccccgggt
    15781 gcgcagcgca tggataatca aggcgtggaa tccgtatata cccaacgaat ggcgggagat
    15841 taagccaagt ccgcgaatgg tacgcgtatc cagcgtgttt ttaaccagag tcaatagcgc
    15901 gattgcgcag ataaaaacca tcggcccaca gtaaggatac caggtatcgg caaaatttcc
    15961 gcgccactgc aattcatata atgtcccgcg agagataata aaaactcccg tcgcaaacag
    16021 tgcggcgctc acccacgaca gtgctttatg ctgtgtgtcc atcatcccta aagcgcggcc
    16081 caacatgcca tacagaatgt agtaaaaagt atcgccattg atatataagt taattggcag
    16141 ccattcaaaa ccgtcgattt tctgcggcac ggtgtttggg ttagcgatga taccaatcac
    16201 caccattagc accagcaaca tttttccgcc gacgttcttc acctgaatca gcggtgaaac
    16261 cagataaatc accgcaatcg cgaagaaaaa ccataagtgg taaaacactg gcttttgcag
    16321 caggtttttc agcgctaact ccacattaat ggaggtaaac agtgcaatgt agagcagcgc
    16381 gattgcgcta taaaaaaaca gacataagcc gatacgcaag aaatggcgcg gctgggcgct
    16441 gcgttcgcca aaaaagagat agccggaaat catgaaaaat agcggcacgc tgacacgaga
    16501 ggcagagttc agaacattgg cgatatccca ggtgacgggg ctaacactat gagcattggt
    16561 cacataccag gtagtggtgt gaatcatcac caccattaaa cacgctatcc ctcgcaggtt
    16621 atcaatccag taaattttgg gctgcatctg tgtctctgta tctggttaaa aaaagtctga
    16681 ccgataaatc attcagttgg cgcactggaa taatctgagt tttaacacta cagcttatag
    16741 aggcttaagg aaattcgtaa gatatcagcc actataccga tataaataat aagactcacc
    16801 tgcaaaccag acggtaattt aatgatgatg aactctttct ttccggcaat ggcgcttatg
    16861 gtgctagtgg gttgttctac accgtcaccc gtgcagaaag cacaacgggt aaaggttgat
    16921 cctctgcgtt cgttgaatat ggaagcgtta tgcaaggatc aggcggcaaa acgttataac
    16981 accggcgagc aaaaaatcga cgtcaccgcc ttcgaacagt tccagggaag ctatgaaatg
    17041 cgcggttata ccttccgtaa agagcagttt gtctgttctt ttgacgcaga tggccatttt
    17101 ttgcatcttt ccatgcgtta agccctgctt tttcccgttt catactgtat atcttccatc
    17161 cagcgggtat actgatccct tcctttaaat ccacacgtat ccagcacgaa ataatatgca
    17221 aaagtttgat accaggacct tccagggctt gatcctgacc ttacaggatt actgggctcg
    17281 ccagggctgc accattgttc aaccattgga catggaagtc ggcgcgggaa cctctcaccc
    17341 aatgacctgt ctgcgcgcgc tggggccaga accgatggcg gctgcttatg ttcagccttc
    17401 tcgtcgcccg accgatggtc gctacggcga aaaccccaac cgtttacagc actactatca
    17461 gttccaggtg gtcattaagc catcgccgga caatattcag gagctgtacc ttggttctct
    17521 gaaagagctg ggcatggacc cgactatcca cgatatccgt ttcgtggaag ataactggga
    17581 aaacccgacg ctgggtgcct ggggactggg ctgggaagtg tggctaaacg gcatggaagt
    17641 gacgcagttc acttacttcc agcaggttgg tggtctggag tgtaaaccgg ttaccggcga
    17701 gatcacctac ggtctggaac gtctggccat gtacattcag ggcgtagaca gcgtttacga
    17761 cctggtctgg agcgacggcc cgctgggtaa aaccacctac ggcgacgtgt tccatcagaa
    17821 cgaagtggag cagtccactt acaacttcga atacgcggat gtggacttcc tgttcacctg
    17881 ctttgagcag tacgagaaag aagcgcagca gctgctggcg ctggaaaatc cgctgccgct
    17941 gccagcttac gagcgtattc tgaaagccgc ccacagcttc aacctgctgg atgcgcgtaa
    18001 agccatctcc gtcaccgagc gtcagcgcta cattctgcgc attcgcaccc tgaccaaagc
    18061 agtggcagaa gcatattacg cttcccgtga agccctaggc ttcccgatgt gcaacaaaga
    18121 taagtaagag gcggctatgt ctgagaaaac ttttctggtg gaaatcggca ctgaagagct
    18181 gccaccaaaa gcactgcgca gcctggcaga gtcctttgct gcgaacttta ctgcggagct
    18241 ggataacgca ggcctcgcac acggcaccgt tcaatggttt gctgctccgc gtcgtctggc
    18301 gctgaaagta gctaacctgg cggaagcgca accggatcgt gaaatcgaaa aacgcggccc
    18361 ggcgattgcc caggcgttcg acgctgaagg caaaccgagc aaagcggcag aaggttgggc
    18421 gcgtggttgc ggtattaccg ttgaccaggc tgagcgtctg actaccgata aaggcgaatg
    18481 gctgctgtat cgcgcccatg tgaagggcga aagcactgaa gcactgctgc cgaatatggt
    18541 tgcgacttcg ctggcgaaac tgccgattcc gaaactgatg cgctggggtg caagcgacgt
    18601 gcacttcgtg cgtccggtgc acaccgtgac cctgctgctg ggcgacaaag tcattcctgc
    18661 aaccattctg ggcattcagt ccgatcgcgt gattcgcggc caccgcttta tgggcgagcc
    18721 ggaattcacc atcgataacg ccgatcagta tccggaaatt ctgcgtgagc gtgggaaagt
    18781 catcgccgat tacgaagaac gtaaggcgaa gattaaagcc gatgccgaag aagcagcgcg
    18841 taagattggc ggtaacgctg acttaagcga aagcctgctg gaagaagtgg cttcgctggt
    18901 ggagtggccg gtcgttctaa ccgcgaaatt tgaagagaaa ttcctcgcgg tgccgtctga
    18961 agcactggtt tacaccatga aaggtgacca gaagtacttc ccggtgtatg cgaacgacgg
    19021 caaactgctg ccgaacttta tcttcgttgc caacatcgaa tcgaaagatc cgcagcaaat
    19081 tatctctggt aacgagaaag tcgttcgtcc acgtctggct gatgccgagt tcttcttcaa
    19141 caccgaccgt aaaaaacgtc tggaagataa cctgccgcgc ctgcaaaccg tgctgttcca
    19201 gcaacagctg ggtacgctgc gtgacaagac tgaccgcatc caggcgctgg ctggctggat
    19261 cgccgaacag attggcgctg acgttaacca cgcaacccgt gcgggcctgc tgtctaagtg
    19321 tgacctgatg accaacatgg tcttcgagtt caccgacacc caaggcgtta tggggatgca
    19381 ctacgcgcgt cacgatggcg aagcggaaga cgtagccgtc gcgctgaatg agcagtatca
    19441 gccgcgcttt gccggtgatg acctgccgtc caacccggta gcttgtgcgc tggcgattgc
    19501 tgataagatg gatactctgg cgggtatctt cggtatcggt cagcatccga aaggcgacaa
    19561 agacccgttt gcgctgcgtc gtgccgcact tggcgtgctg cgaattatcg ttgagaagaa
    19621 cctcaacctt gatctgcaaa cgctgaccga agaagcggtg cgtctgtatg gcgataagct
    19681 gactaatgcc aacgtagttg atgatgttat cgactttatg ctcggtcgct tccgcgcctg
    19741 gtatcaggac gaaggttata ccgttgacac catccaggcg gtactggcgc gtcgtccgac
    19801 tcgtccggct gatttcgatg cccgcatgaa agcggtatcg cacttccgta ccctggaagc
    19861 agctgcagca ctggcggcgg cgaacaaacg tgtatctaac atcctggcga aatctgacga
    19921 agtgctgagc gatcgcgtga atgcctctac tctgaaagag ccggaagaaa ttaaactggc
    19981 gatgcaggtt gtggtgctac gtgacaagct ggagccgtac tttgcggaag gtcgttacca
    20041 ggatgcgctg gtcgaactgg cagagctgcg tgaaccagtc gatgccttct tcgataaagt
    20101 gatggtcatg gttgatgaca aagaattgcg tatcaaccgt ctgaccatgc tggagaaact
    20161 gcgcgaactg ttcctgcgcg ttgcggatat ttcgctgttg cagtaataac gccgttatta
    20221 aaaagcctgc catctggcag gcttttttta tcgcta
//
LOCUS       DBFIFM010000071        20150 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.071, whole genome shotgun sequence.
ACCESSION   DBFIFM010000071 DBFIFM010000000
VERSION     DBFIFM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.071"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(138..884)
                     /gene="cutC"
                     /locus_tag="N7C74_002248"
     CDS             complement(138..884)
                     /gene="cutC"
                     /locus_tag="N7C74_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HGU2432110.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MSRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(898..1464)
                     /gene="yecM"
                     /locus_tag="N7C74_002249"
     CDS             complement(898..1464)
                     /gene="yecM"
                     /locus_tag="N7C74_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU2432111.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPR
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1680..3413
                     /gene="argS"
                     /locus_tag="N7C74_002250"
     CDS             1680..3413
                     /gene="argS"
                     /locus_tag="N7C74_002250"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HGU2432112.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTHDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3455..3499)
                     /locus_tag="N7C74_002251"
     CDS             complement(3455..3499)
                     /locus_tag="N7C74_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432113.1"
                     /translation="MPIFRIHLDGNKKA"
     gene            3590..4078
                     /gene="yecT"
                     /locus_tag="N7C74_002252"
     CDS             3590..4078
                     /gene="yecT"
                     /locus_tag="N7C74_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HGU2432114.1"
                     /translation="MFKFLVLTLGIISCQVYAEDTLIVNDHDISAIKDCWQKNSGDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSNPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4198..4590)
                     /gene="flhe"
                     /locus_tag="N7C74_002253"
     CDS             complement(4198..4590)
                     /gene="flhe"
                     /locus_tag="N7C74_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HGU2432115.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELDGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPSLKVQRNEVIVNYR"
     gene            complement(4590..6668)
                     /gene="flhA"
                     /locus_tag="N7C74_002254"
     CDS             complement(4590..6668)
                     /gene="flhA"
                     /locus_tag="N7C74_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HGU2432116.1"
                     /translation="MSNLAAMLRLPTNLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNAVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6661..7809)
                     /gene="flhB"
                     /locus_tag="N7C74_002255"
     CDS             complement(6661..7809)
                     /gene="flhB"
                     /locus_tag="N7C74_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HGU2432117.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPHMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHVEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8011..8655)
                     /gene="cheZ"
                     /locus_tag="N7C74_002256"
     CDS             complement(8011..8655)
                     /gene="cheZ"
                     /locus_tag="N7C74_002256"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HGU2432118.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRKFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8666..9055)
                     /gene="cheY"
                     /locus_tag="N7C74_002257"
     CDS             complement(8666..9055)
                     /gene="cheY"
                     /locus_tag="N7C74_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HGU2432119.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9070..10119)
                     /gene="cheB"
                     /locus_tag="N7C74_002258"
     CDS             complement(9070..10119)
                     /gene="cheB"
                     /locus_tag="N7C74_002258"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HGU2432120.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLNQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10122..10982)
                     /gene="cheR"
                     /locus_tag="N7C74_002259"
     CDS             complement(10122..10982)
                     /gene="cheR"
                     /locus_tag="N7C74_002259"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HGU2432121.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFASLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11001..12602)
                     /gene="tap"
                     /locus_tag="N7C74_002260"
     CDS             complement(11001..12602)
                     /gene="tap"
                     /locus_tag="N7C74_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HGU2432122.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTTLNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEIIHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12648..14309)
                     /gene="tar"
                     /locus_tag="N7C74_002261"
     CDS             complement(12648..14309)
                     /gene="tar"
                     /locus_tag="N7C74_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HGU2432123.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVDAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDHVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14452..14955)
                     /gene="cheW"
                     /locus_tag="N7C74_002262"
     CDS             complement(14452..14955)
                     /gene="cheW"
                     /locus_tag="N7C74_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HGU2432124.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14976..16940)
                     /gene="cheA"
                     /locus_tag="N7C74_002263"
     CDS             complement(14976..16940)
                     /gene="cheA"
                     /locus_tag="N7C74_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HGU2432125.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKASEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16945..17871)
                     /gene="motB"
                     /locus_tag="N7C74_002264"
     CDS             complement(16945..17871)
                     /gene="motB"
                     /locus_tag="N7C74_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HGU2432126.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17868..18755)
                     /gene="motA"
                     /locus_tag="N7C74_002265"
     CDS             complement(17868..18755)
                     /gene="motA"
                     /locus_tag="N7C74_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HGU2432127.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRIIADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18882..19460)
                     /gene="flhC"
                     /locus_tag="N7C74_002266"
     CDS             complement(18882..19460)
                     /gene="flhC"
                     /locus_tag="N7C74_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HGU2432128.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19463..19813)
                     /gene="flhD"
                     /locus_tag="N7C74_002267"
     CDS             complement(19463..19813)
                     /gene="flhD"
                     /locus_tag="N7C74_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HGU2432129.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITLLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4822 a         5548 c         4910 g         4870 t
ORIGIN      
        1 cactgtcacc cgctcaacag atcgcggtaa tcgttcattt tttatactat tcagggataa
       61 aaaaaggccc ctgttgaaat tgcaggggcc tggtacgagc aagcatcata ttgggcgaca
      121 tgatgcaacg gtaaaaatca tttggcctga tggcgttcaa tgattccttt catttcagca
      181 accgccgccc cgtctacgac ataacgcgaa tactcgtccg cgtgtgcatc tgatgacatg
      241 gacaatcctt gattacgata acgcatcggt gaggcttgcc acgctcccgc ggagctatgg
      301 acttccagca ctccggcatc gaggaagtgg tgcaggtttt ctgcacggac tcctgctccg
      361 gccataatga ttggagcatc acgatgggca ataagttcca taatttttga taaaccttgc
      421 agtgcgtctg atttttgccc tgatgtcagt acccgggcaa tgcccaattc cgcaagatta
      481 ttgagtgtat ttagagggtt agcgcacata tcaaaggcgc gatgaaaagt cactgccagc
      541 ggaccggcag cagccattat tttttccatt cgtgacatat cgacattacc gtcaacatcg
      601 agcacgcccg tcacgagtcc aggaaaacct aattcgcgga ccgtgcgcac atcctcaaga
      661 atggcggcaa actcaccgtc gctgtagcaa aaatcaccac cgcgtgggcg aattatcgga
      721 tgcacaggga tcgtcacccg ctggcgcacg gatttcagta cacccaacga cggcgttaag
      781 cccccctctt ttggggctgc gcataattca actctgtctg cgccgttttg ctgcgccgtt
      841 agcgcgcatt ccatgctgta acagcaaatt tccagtaatg ccatttttgc tccttaatta
      901 cgccgactgc tcgctggcaa caatctcttc aatggaccac ggatgaaatt taatggtcgt
      961 tttgccatcg gtgaccgcca gcgtcggatt cggtaatcgt tcatgctcac cttttggcga
     1021 actggtttta actgagatcc ccggtaggct gagtccttca tcagaaagca atgccagcgc
     1081 acgggcattc agcgtttctg gatcacccgg caaaacgatt tcaatatgtt cccaaccttc
     1141 gtgcgggtaa cgtttttccc taggccacgg tagctccaca atagaaaact gccagtgtgc
     1201 aacctgtacc ggttcatgca atttaaacag acaaatcggt ctgccattga tcatattttc
     1261 tgacaaaagc tcgccacact gttcaaaccc gcgacgccag cgctcagcag tggcgttttg
     1321 atggcaacgc aaagaaatgt gatcggcagt cagcggagtg atattcaacc ccagacggcg
     1381 ggaaagttca tctaatgcgt gggtaaatcg cggtaaatcc gatgcaatat cctgcagctc
     1441 gtcgatagat tgccagttcg ccataatcac tcttcgtctt tcagtaaaag cgttaattta
     1501 ccctgttgcc ctgcgccaac caaccgctga tttcacgccg cttctgatgc aatagtgaaa
     1561 acggcaatac gccacgcgca cgttgctgac gaaaacagcc atttgcagta tactcccgcc
     1621 ctaatttctt taactggtgc gggcaatttt tgctcgcttc atcaatgtaa ggtattccgg
     1681 tgaatattca ggctcttctc tcagaaaaag tccgtcaggc catgattgcg gcaggcgcgc
     1741 ctgcggattg cgaaccgcag gttcgtcagt cagcaaaagt tcagttcggc gactatcagg
     1801 ctaacggcat gatggcagtt gctaaaaaac tgggtatggc accgcgacaa ttagcagagc
     1861 aggtgctgac tcatctggat cttaacggta tcgccagcaa agttgagatc gccggtccag
     1921 gctttatcaa cattttcctt gatccggcat tcctggctga acatgttcag caggcgctgg
     1981 cgtccgatcg tctcggtgtt gctacgccag aaaaacagac cattgtggtt gactactctg
     2041 cgccaaacgt ggcgaaagag atgcacgtcg gtcacctgcg ctctaccatt attggtgacg
     2101 cagcagtgcg tactctggag ttcctcggtc acaaagtgat tcgcgcaaac cacgtcggcg
     2161 actggggcac tcagttcggt atgctgattg catggctgga aaagcagcag caggaaaacg
     2221 ccggtgaaat ggagctggct gaccttgaag gtttctaccg cgatgcgaaa aagcattacg
     2281 acgaagatga agagtttgcc gagcgtgcac gtaactacgt ggtaaaactg caaagcggtg
     2341 acgaatattt ccgcgagatg tggcgcaaac tggtcgacat caccatgacg cagaaccaga
     2401 tcacttacga tcgtctcaac gtgacgctga cccatgatga cgtgatgggc gaaagcctct
     2461 acaacccaat gctgccagga attgtggcgg atctcaaagc caaaggtctg gcagtagaaa
     2521 gcgaaggggc gaccgtcgta ttccttgatg agtttaaaaa caaggaaggc gaaccgatgg
     2581 gcgtgatcat tcagaagaaa gatggcggct atctctacac caccactgat atcgcctgtg
     2641 cgaaatatcg ttatgaaaca ctgcatgccg atcgcgtgct gtattacatc gactcccgtc
     2701 agcatcaaca cctgatgcag gcatgggcta tcgtccgtaa agcaggctat gtaccggaat
     2761 ccgtaccgct ggaacaccac atgttcggca tgatgctggg taaagacggc aaaccgttca
     2821 aaacccgcgc gggtggtaca gtgaaactgg ccgatctgct ggatgaagcc ctggaacgtg
     2881 cacgccgtct ggtggcagaa aagaacccgg atatgccagc cgacgagctg gaaaaactgg
     2941 ctaacgcggt tggtattggt gcggtgaaat atgcggatct ctccaaaaac cgcaccacgg
     3001 actacatctt cgactgggac aacatgctgg cgtttgaggg taataccgcg ccatacatgc
     3061 agtatgcata cacgcgtgta ttgtccgtgt tccgtaaagc agaaattgac gaagagcaac
     3121 tggctgcagc tccggttatc atccgtgaag atcgtgaagc gcaactggca gctcgcctgc
     3181 tgcagtttga agaaaccctc accgtggttg cccgtgaagg cacgccgcat gtaatgtgtg
     3241 cttacctgta cgatctggcc ggtctgttct ctggcttcta cgagcactgc ccgatcctca
     3301 gcgcagaaaa cgaagaagtg cgtaacagcc gtctgaaact ggcacaactg acagcgaaga
     3361 cgctgaagct gggtctggat acgctgggta ttgagactgt agagcgtatg taatcgattt
     3421 ttcgtgagag tgaagcctga tcaggtttag ccgatcaggc ttttttatta ccatctaaat
     3481 gtattctgaa aataggcatg ccattgtttt ctcgctgttg agtaagaggc cagaagcgta
     3541 atatccagct ccagggaaac ggtaacggtt caatttaagg aataccgcag tgtttaaatt
     3601 tcttgtatta acattaggca ttatctcttg ccaggtttac gcagaagata cgcttatagt
     3661 aaatgatcat gacatttcag ccatcaaaga ttgttggcaa aaaaattcag gtgatgatac
     3721 tgacattaac gtgatcaaat catgtctgcg acaagaatac aatctcgtcg atgcgcaatt
     3781 aaataaagct tatggtgaag cttatcgtta tatagaacaa gtgccacgca caggtgcaaa
     3841 aaaacctgat actgaacaac ttaacttgct taaaaaatca caacgagcct ggctggattt
     3901 tagggacaaa gaatgtgaat taatcctttc aaatgaggac gttcaggatt taagtaaccc
     3961 ttattctgaa tcagaatggc tatcatgtat gatcatacag accaatacgc gaactcgcca
     4021 gttgcagcta taccgtaact ctgaagattt ttatccaagc cctttgacaa gaggataatt
     4081 cacatatttt tggcatgttt tgttgcaagc tattcctgat aaatgattgc aacaaaacat
     4141 ccagcctttt tcactgagtt attaaacata ctcgcgagcg cgtaattttt ttgtccttca
     4201 gcgataattc acaatcactt cattacgttg tactttcagc gacggaatta accgcccacc
     4261 gccaggcact tcccagataa agcgcaacgg ttctgctgcc gctataccgg aaaaggccac
     4321 ggtggttccg ctctgcccat ctaattcgac acaacgagat tgcgaacaca agcgaacccg
     4381 cagtcctgca ggtgtcgggc cgataagctg ataccgccac gccaccagcg tcatcaatcc
     4441 tgaagcaggt tgtcgcgtag aaagaggtgc agacgacatc gactcaccgc gatgatttag
     4501 cgtaattccg acactacttg cctgccacat cccctccccg gcggcttgca ccagcagcgg
     4561 aaacaataat attgctaata aggttctcat catttaccgc caattgtcgc cgtcatgcgg
     4621 atatgtcggt tatcagacag ttccagattc gacagcacca ctaactgcgg caagctgcgg
     4681 cgcagaaagc gagacaataa tggtcgcagc gcgtggttca ccaataaaac tggcggcgca
     4741 cccaacatct cctgacggga tagcgcttcc tgagtttgcg ccagtaaacg atccgccagc
     4801 cctggctcca gtccgccccc gccctgcaac gcctgtagca acaaacgttc cagcggtgta
     4861 tcgaggccaa taacatggac ttcatctttg ccaggaaacc actgctgggt aatcgcccgt
     4921 cccaacgcca cgcgcacgac ggcggttaat tcatgtggat cgctttggat gggcgcatgt
     4981 tccgccagcg tttcgagaat agtgcgcata tcgcgaatcg gtactttttc atcgaggaga
     5041 ttttgcagca ctttatgcag tgtggtgagc gtgacgacgc cagggacgag atcttccgtc
     5101 agctttggca tctcctgggc gacgcgatcc agcagctgtt gcgcctcctg acgaccaaac
     5161 agctctgcgg catgctggct aatgaggtgg ttaagatgcg ttgctaccac cgtgctggcc
     5221 tcaaccactg tgtacccctg aatctgcgcc tgttctttta gcgcactttc aatccagata
     5281 gcattcaggc caaatgccgg atcgacggtc gcctcaccag gtaacgtccc ggcagcggtt
     5341 ccagggttaa tagccagcca gcgccccgga taagcatcac cactgcctat ctccacgcct
     5401 ttcatcaaaa tgcgatagcg ggcaggttgc agatccatat tgtcgcgaat gtgcaccact
     5461 ggcggcagaa atcccatctc ctgggcaaat ttcttgcgga tactgcgtat acggcccaac
     5521 aactcaccat cctgctggaa atcgaccatc gggatcagtc gataacccac ttccattccc
     5581 agagaatctt ccagttgtac atcgttccac gtcgcttcga caacggcatt attctctgcc
     5641 atttttaccg gttttggttc ggcaggcgct ttttgttcgc gtccacggat ccaccaggcc
     5701 agccccagca atccggcagt gaacagcaaa aataccaggt tcggcattcc aggcaccagg
     5761 ccgagtaaac cgagcacggc agcgcttaac aacataacgc ttgggttact gaaaagctga
     5821 ttcaccatct gctcgccaac atcctgatcg gtgctgacac gcgtaacaat gacccccgcg
     5881 gcggtagaaa tcaccagagc cgggatttgt gccaccagac cgtcaccaat ggtcaagagc
     5941 gtataacttt ccgccgcgtg tcccatgctc atgccatgtt gcagcacgcc gaccagcaac
     6001 ccgccgacaa cgttaatgac catgatgagg atcccggcga tggcatcgcc gcgaacaaac
     6061 ttacttgccc cgtccattga gccgtaaaaa tcggcttcct gagtgacttc ggagcggcgt
     6121 tttttcgcct catcttcacc aatcaatccg gcgttaaggt cggcgtcaat cgccatctgc
     6181 ttacccggca taccatcgag aacaaagcgc gcacccactt ctgcgatacg cccggcacct
     6241 ttggtaatga ccataaagtt gatgatcacg agaatgacaa acaccacgat accgatagcg
     6301 aaattgccac caacgaggaa gtgaccgaac gcttcaacca ccttccctgc cgccgctgcg
     6361 ccggtatgcc cttccattaa aatgatacgg gttgaagcca cgttaagtgc cagacgcaac
     6421 agcgtggtaa acaacaaaat ggtcggaaac gcagcaaact caagcgtgcg ctgggtaaac
     6481 atcgccacca gcaacaccat gatcgacaag gcaatattga aggtaaacaa caggtcgagt
     6541 atgaatgcgg gcagtggcag caccatcatc gacaagatca acaggatcaa aatcggtccg
     6601 gcaaggatct gccattgtgt cgatttcagg tttgtgggca ggcgcagcat cgcggccaga
     6661 ttactcatgg gtcggtttct cgttaataaa atccagggct tccggcaccg gaagatgagt
     6721 aggttgtaca gggcgctgtc caccagccag acgccagcgt ttcagttgcc agacccaggc
     6781 cagcacttcc gccaccgcgg cgtacagttg acccgggatt tgttgaccaa tctccacatg
     6841 tcgatacagc gctcgcgcca gcggcggcgc ttcaagcgtc gggacgttat tttcagcgcc
     6901 aatttcacga atgcgcagcg cgaccagccc tgcaccttta gcgaccactt tcggtgcgct
     6961 cattttgttt tcgtcatact gcaacgctac cgaatagtgg gtcgggttat tgacaatgac
     7021 atccgctttc ggcacatcgg ccatcatccg acgccgtgcg gcagctcgct gcatctgacg
     7081 gatccgccct ttaacatggg ggtcgccttc gctttgtttg aactcatcac gaatatcctg
     7141 ccgcgacatt cgcagctttt tcaggtggct gaagatttgg aaaaagacgt caaatcccac
     7201 cattggaatg acaccaagca ccaccagcag tgcgcatagc cctaccaaat ccatcgcatt
     7261 acccatggcg gtaatcggag actcggccat taagcgcatc atatgcggcc agtgatgcca
     7321 gagaaaaaac cccgtcacgc tgccaaccag gatggttttc aaaattgctt taagcaactc
     7381 cgcgccagtc tgtgccgaga acatccgttt tatgcccggt agcgggttga gtttggaaaa
     7441 cttcggctgc aaggattttc cgctaaatac cagccccccc agcataaccg gagaaatgag
     7501 cgccaccagc accacgccgc taatcagcgg tagcagcgcc agcatggctt ctctgatcag
     7561 cagaataatt tgcccgagga tcagattcgg gtctttgata atactgtgat caaaatgcag
     7621 cccagcggag agcatgcccg acaatcgacg ggccagcgac acaccgccaa accagataac
     7681 actaacgccc accagcaaaa tcagcagtga ggtcagttca cgggaacgcg ggatttgccc
     7741 ctcttcccgc gctttttcta gtcggtgagg tgtgggggct tctgttttgt cgtcgctctc
     7801 gtcagacacg tcgccaatcc tggaaagagt attaagcgta aatgatgcca gagcgcaaag
     7861 cgttcaatgg tttgagtaag gggcaaaaca ggcgggattt agggcttttg ctgccacaca
     7921 tcaagcatag tgtgcgtttg tcggatgcgg cgtcatcgcc ttatcagacc gcctgatatg
     7981 acgtggtcac gccacatcag gcaatacaaa tcaaaatcca agactatcca acaaatcgtc
     8041 cacctgatcc tgactggcta ccacaccggc tttgctggta tcgacctgag gtccattaag
     8101 caaactctgg ttttcacgtt ttggacgcga ctcctgttcc gggatgtttt ccaacagcac
     8161 catcagcaac tggcgttcga tctcctgaat gacatccatc atccgcttaa tgacctgccc
     8221 ggtgagatcc tgaaaatcct gcgccatcat gatttccagc agttgcgcgt tagtaaagct
     8281 ggtatgcgcg ggtacatctg ccagaaattt ccgcgtatct gttaccagtt cacgggcgtc
     8341 ggcaaggtca atcggatcgg caaaccagtc atcccaacgt tgggttaacg cttttgctga
     8401 tttctccatt tgatcctgat gcggttgtga cgcctcaaca ctgttcagcg cccgctccgc
     8461 agcctgggcg gtcatctgca caacatagta caaacgatcg cgcgcatcgg ggatggcttc
     8521 cgccgcttcg gcaatggcct gatccagtcc cagttcccgc aaactgtcgc gcagcatacg
     8581 cgtcaggctg ccgatgcgcg caatgatatc gccagctgaa tgctcgtcag caggtttgat
     8641 tgatggttgc atcatagtcg catcctcaca tgcccagttt ctcaaagatt ttgttgagtt
     8701 tttcctccag cgtcgcggcg gtaaatggct tcaccacata gccactggcc cccgcttgcg
     8761 ccgcagcaat gatgttctct ttcttcgctt ctgcagtcac cattaacact ggcaatgccg
     8821 acatcgcgcc atccgcacga atcgttttca gcaattccag gccatccatg ttgggcatgt
     8881 tccagtcgga gataacaaat ccataaccgc ctgcctgcaa cttattgaga gcgtcgacgc
     8941 catcttccgc ttcctcaaca ttattgaatc ccagctcttt cagcaggtta cgcactatgc
     9001 gtcgcatggt ggaaaagtca tccacaacca aaaatttaag ttctttatcc gccatttcac
     9061 actcctgatt taaatacgta tcgcctgtcc ggcactaatt tttgccaaca tttgctggct
     9121 tacctggtta agatcgacca cttcgcagac accacccata ttgatggcct cgcgcggcat
     9181 gccgaacacc acgcaacttg cttcgttttg cgcaagggtc catgccccag cctgacgcat
     9241 cgccaacatt cccgccgcgc cgtcgttgcc cataccggtc aggatcaccc caaccgcatt
     9301 acgccccgcc tgtttggcga cagaatggaa caacacatct accgaaggcc gatgacggtt
     9361 aaccgccggg ccatcgtgaa ttttgatttg gtaatttgcg ccactacgcg ccagctccat
     9421 atgccgatcg cccggcgcaa tataggcatg ccccggcaaa acacgttccc cgtcttcggc
     9481 ttctttaacc ccgatctggc aaagcttatt aagtctgtcg gcaaaagagc gggtgaaacc
     9541 gggcggcata tgctgggtaa ttaacagtgc cgggctggaa agcggcaacg gttgcagtac
     9601 gtgacgaatt gcctcagttc cacccgttga agcaccaatc gcaatcagtt tttcagaact
     9661 caacaacggc cctgccttca gcgttgtcgg tgccgacaat ggcttatgtg ctgcaaggct
     9721 cgcctttgct gctgtacgca ccttttcagc aatcatttcg ctgtacgcca gcattccttc
     9781 gcgaataccc agttgcggtt tggtgacaaa atctattgcc cccagctcca gcgcgcgcag
     9841 cgtgacttct gatcctttgc cggtcaggga agaaaccatc acaacgggca ttggacgcaa
     9901 acgcattaat ttttcgagga aatccagtcc gtccatccgc ggcatttcaa catccagcgt
     9961 cagcacatcg ggattgaatt tcttaatcaa gtcacgcgcg accagcggat caggcgcggt
    10021 cgccaccatt tccatgtcgc tatggctgtt gatgatttct gtcatgatct ggcgcatcag
    10081 tgccgaatca tcgacagata acaccctgat tttgctcatc gttaatcctt acttagcgca
    10141 tacaccgttt gaccacgcag cgtgaagcgg cgctcgaggt ggctaaagtt ttcagagtga
    10201 cccgcaaaca gcaatccgtc gggtttaagg agcggaacaa agcggcgcaa aatctcctgc
    10261 tgggtagttt gatcgaagta gatcatgacg ttacgacaga agatcgcatc aaacggcccc
    10321 ggcacggtgt actgtttcgc cagtagattc agcgaggcaa aatcaacata gttcgccagc
    10381 tcctgacgca cgcgtaccag cccttcatgc ggtcccgtcc ctcgcatgaa ataccgttgc
    10441 agttgctgcg gcgtcaggtt tttcaactct tcatggcgat agataccgct tctggctttt
    10501 tccagcactt cggtgtcgat atcactggca aacactttcc agcgtccggg cgcggtgccc
    10561 aatgtgtcag ccagcgtcat cgcaatgctg tacggctctt cgccggtcga agccgccgcg
    10621 ctccatacgc gatactcgcc agaacggcga cgtgcgtgat ccgcgagcag agggaaatga
    10681 tgtgcctcac ggaaaaatgc cgtcagattc gtggtcagcg aattgataaa cgcctgccac
    10741 tcaccgctgt gctgattaga ttccagcaag ttcagataat gaccgaaatc cgtcagtccc
    10801 agcgaacgca aacgacgaac cagtcggttg taaaccatgt cgcgtttatg gtcagccaga
    10861 acgatcccgg ctcgttgata gatcaattga cttatccgcc gaaaatgcgc gtcggaaagc
    10921 gccaggcgct cggtcatctg taacaataaa gacgtttgcc cacagggcag agatgaagtc
    10981 atagcgcctt ctcaatcact tcaggatacc actggcgcaa tttgtaactg cgccgactca
    11041 tgtcgtgcta cttcatgttc ttcaagggta aataccgcca cacgcgacga aagatggtcg
    11101 gcctgattcg ccagttgttc cgttgccacc gccgcttctt ctaccagcga ggcgttctgc
    11161 tgtgtcacct gatccatctg gctgacagcc tgcgcaacct gctcaatccc ccgccgttgt
    11221 tcttccgagg ccgaagcaat ttctcccata atgtcgttca cgcgggtcac cgaactgaca
    11281 atatcggtca tggtcgcggc ggcgttattc accagtttcg aaccctgctg gacacgattg
    11341 actgactctt cgatcagccc tttgatctct tttgccgcct gggcgcttcg gctggcaaga
    11401 ttgcgcactt ctcctgccac taccgcaaaa ccacgcccct gctctccggc gcgagccgct
    11461 tctactgccg cattcagggc cagaatattg gtctggaaag caattccgtc gataacgctg
    11521 ataatgtcgc caattttttg cgagctggtg gcgatctcct gcatggtgtg agtcatggta
    11581 ctgacctgaa caccgcccgc ctgcgccgtt gtcgcggcat tttttgcaag ttccgacgcc
    11641 tgtcgtgcgt tatcggcgtt ttgccctacc gtggcggtta attgctccat actggcggcc
    11701 gtttgtgcca gcgatgccgc ctgctgttcg gtacgacttg agagatcgtt attgcctgcg
    11761 acaatctccg cgataccaat gtgcatctcc tggcttccct tacgcacatc acttaccgtc
    11821 ccacgcaaag cctgctgcat ggtcttcagg ctggcaaaaa tggcggtgat ctcattacga
    11881 ccatataccg caatcggacg cgccagatta cccgcagcaa tgctgtcaaa atggctaccg
    11941 ataatggcca gtggttgaac aatcatcttg cgcgtccacc acagcgcact gctgatgtag
    12001 atcgccgcaa caataatcat gctgataaac accagcgccg aaatctgata gttacgctgg
    12061 ctttgcgcac tggcggcttc cagcacatgg atgatctcca gctgccaggc ctcaaagtta
    12121 acgtcaaacg cattctgtga tccctgcacc ggcgcagtga ggaaatccga aagttgatta
    12181 ctttcaagcc aggtcgcctg atgttcgaga tcgttgtgcc agcgggcaaa acttttctcc
    12241 gtctcttttt gcaatgccct gacgtgctcg ttgcccgcag tcatcgccat aaaactttta
    12301 aacagcgtgg tggattgcgt cagactggca cgcgccgtcg tcatcaacgt tttaatgtca
    12361 tccgccggat agctaagcgc tgtcagagtg cctgctttgt tcagcgtggt actggcctgt
    12421 aacattaccg cccgagtttg cgctaatgcc gcacgttgct gattgctctg ctcgacctga
    12481 ttcagtcgtt gcaaatcgtc gcgaaatgcc caaaaagaca tgccgttact gccaatctgc
    12541 aagatcccgc agagaatcaa aattaaaaac agcgtggtcg aaattcgaat acgattaaac
    12601 atcaacgctc cccatcaggc ggcaatgacc gcgttagtaa atactcgtca aaatgtttcc
    12661 cagtttggat cttgttcagc aattcgcagt cgtggctgtg ccggtggttg ctcactggca
    12721 ggacgggatg gtgtttgcgg tttattggtg agtgggctgg ctgccagacg aaacgcggaa
    12781 accgcttgcg ttaaacgact agcctgttct tccagcgcag ctgcagcggc agctgattcc
    12841 tgcaccagcg atgcgttctg ttgcgtgacg tgatccattt ccgaaaccgc caatgcgact
    12901 tgatcaatgc cacggctctg ttcatccgat gccgatgcaa tctcgcccat aatgtcagtc
    12961 acgcgagtga cagcatcgac gatattgttc attgtttccc cggcgctttc gaccagcacc
    13021 gaaccggtat caacgcgcga gacggagtct tcaatgagag ctttgatctc ttttgccgcc
    13081 tgggcgctac gactggcaag attacgcact tcgcccgcca ccacggcaaa accacggccc
    13141 tgttcacccg ctcgcgcggc ttcaacagcg gcattcagcg cgaggatatt agtctggaag
    13201 gcaataccgt cgattacgct gataatgtcg gcgattttct tcgaactatc ggcgatctca
    13261 tgcatcgttt tcactacgcc atccaccact ttgccgccgt gctgggcggt gtcggaggca
    13321 ctttgcgcca gttgcgaggc ctggcgggcg ttatcggcgt tttgcttcac tgtcgcggtg
    13381 agctgctcca tgctggcggc agtttcttcc agcgcggatg cctgctgttc cgtacgggag
    13441 gaaagatcgg tgttgcccgc cgcaatttca cgggtaccgg catagatggc atctgaacct
    13501 tcgcggacat gagtgacggt gtcagtcaaa gagcgttgca tatgtgaaac gctctgcgcc
    13561 aggtcgccca tttcactgcg cccgtcaatg gtcagggtat tcgccaggtt accaccggcg
    13621 atttcgcgaa tgtgagcaat aatttttgcc agcggtgtaa gcaacatacg gcgaatgcca
    13681 taccacgcca ccagcagaat caataccacc accagcgcga taaccgccag ttgccactgg
    13741 gcaaatcggt aatcatctgc gttgtcagtg acgatatcgc gatacagttt ttcactgctg
    13801 agggcgtact gagcaaacgc ttcgcccatt gcattttgca ttccctgggt tggctgagcg
    13861 aaataagctc cagtattgcc ataatcaaga taatcaatca gttcagttaa cgctgtgtaa
    13921 tagtttttat atttttcatc aatattacga ctggtagcga ccatttcagg taacggtgcc
    13981 atgcttttga attttttata atgagtcgcg gcctgggcca atgttttcct ggcgctatcg
    14041 agcaattcaa ctttggcatt actttgttga ttggaggaat ccatcatcat tcgcaccgct
    14101 gaacgactca ggttaatgcg cgtttgcagc attaaatccc aggttgacgt cagctcgccc
    14161 tgctgttctc gtaattgatt ggaaaccaca aagctcttct ggctatggtg aagggaagaa
    14221 aaaaacagac tgccggaaat aagctgtaac agtgcgaata cccccagcac cattaccaac
    14281 agcgtgacta cgcggatacg gttaatcata aggcaccttc ctgaaaacaa gttgatctcg
    14341 ttatcggcaa ggaaggggga aactttattg ctgatgccac ccgccgcgaa attgaaataa
    14401 aacccgatgc gcagatcatc gggttcattt caattgagga aatcaggaga attacgccac
    14461 ttctgacgcc gcgctatcta acagcgccat ctcttcgctg ttcagcagtt tttcgatgtt
    14521 caccagaatc aacatacggt cgcccagtgc gcccagtcca gtgagatatt ctgttgaaag
    14581 cgtcacggca aactctggtg ccggacgaat ttgctccgcc gtcaatgaaa gcacgtctga
    14641 aacgccgtca accacgatgc cgaccacccg ctgtccgaga ttcaggacga taactaccgt
    14701 gttgtcgtta tagtccacat ccacctggct gaacttaatt cgtaagtcaa caatcggcac
    14761 aataacgccg cgcagattcg tgacgccttt gataaacgct ggcgtgttcg caatccgtgt
    14821 tacctgatcg tagccacgga tctcctgcac tttcaggata tcaataccgt actcttcatc
    14881 accaagggta aataccagaa attcctggcc tgacggctcg ctggccagct ttgttacatt
    14941 cgtcataccg gtcatattgt taccttttta ctcattcagg cggcggtgtt cgccatacgt
    15001 tgttcgcggt ttatcgcctg caaggcggag acatcaacaa tcagtgccac gctgccgtcg
    15061 ccaagaatgg tcgcagcaga aatgccgggg actttgcgat agttactttc aaggttttta
    15121 actacaacct gatgttgacc aattaattga tccaccagca aggcgtagcg gcgaccgcca
    15181 ctttgtaaga tcaccacaat tccctgggta gcttcggttt tcgcgcccgc gacgttgaac
    15241 actttccaca gttcgacgat gggcagatat tcaccccgca cttccagcac ccgctcgccg
    15301 ccggccagtg gatgcagatc ggcttcacgg ggttgcagtg attccataac agcattcagc
    15361 ggcagaatga atacttcatc cgcaacgcgt acggacatgc cgtcgaggat ggccagcgtc
    15421 agcggcagta aaatgcggat cgtagtgcca gtaccctgct tcgactggat ttcaacatga
    15481 ccgcccatct cctggatatt acgtttaacg acgtccatgc cgacgccgcg cccggagacg
    15541 tcggtgacct gctctgccgt ggagaagcct ggtgcaaata tcagcatcgc gacttcgtcg
    15601 tcgctcatgt tttcgctgac agtcaaacct tgcgaggccg cttttgccag aattcgctca
    15661 cggtttagcc ccgccccatc gtcggtcact tcaatgcaaa tgttgccgcc ctgatgttcg
    15721 gcagacagaa ttaaatttcc gacgctgttt ttacctgcgg cgagccgttt ttctggcagt
    15781 tcaataccgt gatcgaggct attgcgtacc aggtgggtca gcgggtcgat aatgcgttct
    15841 atcaggctct tgtcgagctc ggtggaactg cccaccagcg tcagttctac ctgcttgccg
    15901 agttttcccg ccagatcacg caccagccgg ggatagcgac taaagacata ttccatcggc
    15961 atcatgcgaa tcgacatcac tgattcctgc aaatcacggg cgttacgttg taactgcccc
    16021 atgctggtaa tcaaatcacc atgattaacc gggtccagtt cgctggaacg ctgggcaagc
    16081 atggactggg tgataaccag ctcgccgacg aggttaatta attgatcaac cttttctacc
    16141 gctacacgga tgctggtgga ttcactgcta cgcgtcgttt tttcccgctc cacgcgaccg
    16201 gttggcgctt gttcggctgc cagtttaagc actggtgggg tggatatttt tggcgagact
    16261 tctactgttt caaaggtaat ctgatcggct tcaatcacaa aacagagtac cgctgtgatg
    16321 tcatcttcgg cgatatcgcc cggtaatatt gccgagagag aatccgcccc tttcaccacg
    16381 tcagttaacg ttgtcagatg ccccagctct tcttccagca ggtcgacttc gctggccttc
    16441 aggcgcgaaa ggataattcg tcgcggcaac tgactgcgac tctgctcatc ttgcggttca
    16501 cttttggcaa ccacacttaa tcgggtcact gcggatggcg tttcgccttt cgcttctaat
    16561 gccagttgac gcaaggcctg gcagatataa tcgaagctgg cggcatccgg ctcttgcgac
    16621 tgtttataag cgtcgagctg ttcttgcatg atgtccttcg tttccaaaaa cagattgata
    16681 atgtcggtgt tgagttgcat ctcacctcgt ctggcttcat cgagcaggtt ttccatcaga
    16741 tgcgtggttt cctgcaaaac gctgaagcca aaagttcctg cccctccttt gatcgagtgg
    16801 gcagcccgaa agatggcatt caattgttcg gcatctggcg cttccggctg caaaaccagc
    16861 agatgctgct ccatatcagc caacagttcg tccgcttcat caaaaaatgt ctgataaaaa
    16921 tcgcttatat ccatgctcac gctgtcacct cggttcggct gatggcattg tgggaacact
    16981 gacctgtggt gcaacctcag gtttttccag ggcgcttact ggctcattct ggctttcggc
    17041 gttttcatgc aaaatggcct gttcggcttg tttgttcagt accagcaggc tgatgcgacg
    17101 gttgacggca tcatcaggtc cgcgatcgct taagcgcatc gttgccgcca tgccgaccac
    17161 acgtaacact ttgccgctat ccaaccctcc gaccatcagt tcgcggcggg atgcattggc
    17221 ccgatcggca gaaagctccc agttgctgta acctttctca ccactggcgt aggggaaatc
    17281 atcggtatga cccgaaaggc taatacggtt gggaatacca ttcagaacgg gcgcgatggc
    17341 acgcagaatg tcgcgcatat agggttcgac gtcggcactg ccagttctaa acatcgggcg
    17401 attctggcta tcgatgatct gaatacgtag accttcctgg accagatcga ttttgagatg
    17461 tggacgtaac gcccgcagtt tcggatcgga ctctatcaat tggtcgagat cgccacgcaa
    17521 tttccgcaat cgactttgct ccatgcgttt tttcaactct tcgatgttcg gctgcttatt
    17581 cacttccccc tggctttggg tgtaatcatc gccaccgccg ggaattgggc tttcactatt
    17641 agaaatgcga tcgccgcccg taaccgcagt cgccagtgga gtccggaagt actccgcaat
    17701 ctgaatcaac tcttttgggc tggagatgga gatcagccac atcaccagaa aaaaggccat
    17761 catcgcggtc ataaagtcgg cataagcaat cttccacgat ccatgtgctg ccccgtggct
    17821 tttggctttg cgtcgtttga cgacaataat cggatgcgct tgattcttca tgcttcctcg
    17881 gttgtcgtct gttgttgcgg atttttcacc gcacgcacat gctcttccag ttcaataaac
    17941 gacggacgtt cgctggaata gagcgttttg cgaccaaact caacggcgat aggcggtgcg
    18001 taaccgttca gattagaaag cagtgtgact ttgacgcact gcatcatttt gctggtttcg
    18061 gcgcttttct gacgtaaaac actcgctaat ggggaaataa atccgtaagc caataaaatg
    18121 ccgaggaaag tccccaccat cgcatgtgcg ataagcgccc ccagctcggc ggcaggacga
    18181 tcggctgaac ctaacgcgtg aacgaccccc attacagccg cgacaatacc aaacgccgga
    18241 agtgagtccc cgaccagcgc cagactgttc gccgggactt ctgcctcgct ttcgtgcgtc
    18301 tcaatctctt catccatcag ggcttcgatt tcgaaggtgt tcatgtgacc gctgataatc
    18361 aggcgcagat aatcgacgat aaaatcaagc atgacgctat ccgcgatgat gcgtgggtag
    18421 ctggcgaaga tctcgctctc acggggattt tcaatatcac gttccagcga aaacatcccc
    18481 atctgccgcg atttcgccat caaccgataa agcagagcca gcagatccat atacattgct
    18541 ttggtgtatt tggagcgacg aaacagcaac ggcagcgctt tcagcgtgcc tttaatcgct
    18601 tttccattat tgccgacaat aaacgaccca atccctgcac cggcaataat caccagttca
    18661 gcgggttgat agagtgctcc aaggcttcca ccggtcatca aataaccgcc gaaaactgta
    18721 ccgagaacaa ccaggtaacc taataagata agcacgacat catccttcca ctgttgacca
    18781 tgacaggatg ttcagtcgtc aggcgttaac gcgcgattgg ggcaaaaaaa agcagcggta
    18841 cgtcgttacc gctgctggaa tgttgcgcca caccgtatca gttaaacagc ctgtactctc
    18901 tgttcatcca gcagttgtgg gataatatcg gcaggattct gggaaagttt acgtcttttt
    18961 actgcccggg atggcggttg acataagctg caggcaaagc tgccaacagg ctggtgagcg
    19021 tgggtaataa aattgccgcc gcagcagttg cagctggaaa gttgcagtaa tccactttca
    19081 acaaaccgca ccaatgtcca ggcacgggtt aatgccagca gcggtccttc ttctgcttgc
    19141 gggcactgtt caaggtataa acggtaggct ttgatcaccg catcgacgcc attacacaaa
    19201 ccggttttca gtaaaaactg ccatgcatta cagaacatcg aagcatgaac gttttgttcc
    19261 caagtcataa accagtcggt tgagaatggc agcatgcctt tcggcggtgg gcttccgcgc
    19321 agttctttat aaagttttat caggcgtccg cgacttaact gtgtttcgct ttccagcatc
    19381 tgcaaacgag cgcccagggt gatcaattcc attgccagct gaatatcccg cgcttcctga
    19441 acaatgcttt tttcactcat gatcaggccc ttttcttgcg cagcgcttct tcaggctgat
    19501 taacatcatt cagcaagcgt gttgagagca tgatgccggt atgaatttgc tggagatcgt
    19561 caacgcggga atcttgcgtc aacagagtaa tcgtctggtg gctgtcaaaa cggaagtgac
    19621 aaaccagctg attggtttct gccagcttaa ccatttgcgg aagagtcagt gccgctaatg
    19681 ttgtcgccat ttcttcattt atgccgagac gaaacatagc ggacgctttg tcctgaacaa
    19741 tcagacgctg tgcaagtagt aaatatgaca agttgatgtc ataaatgtgt ttcagcaact
    19801 cggaggtatg cattattccc acccagaata accaacttta tttttatgcg gtttcaccgc
    19861 accccgtgat gtcgccggga agccccggta aaaaataatt agcattagaa tagttgcgat
    19921 aagctgcaat aagcagagcc acctttttgg tttaataagt ccttacaaat agaagggggt
    19981 ctttacactt atctaagatt tttcctaaat cgacgcaact gtactcgtca ctacacgcac
    20041 atacaacgga ggggggctgc gattttcaat aatgtgtgat gcagatcaca caaaacactc
    20101 aattacttaa cataaatgta taagtcatac ttttgttttg ggtgtatttc
//
LOCUS       DBFIFM010000072        19878 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.072, whole genome shotgun sequence.
ACCESSION   DBFIFM010000072 DBFIFM010000000
VERSION     DBFIFM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.072"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..584
                     /gene="dosC"
                     /locus_tag="N7C74_002268"
     CDS             <1..584
                     /gene="dosC"
                     /locus_tag="N7C74_002268"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HGU2432130.1"
                     /translation="FLLQIRNTVSQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPT
                     IFKREIAHANRTGTPLSVLIIDVDKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDY
                     VFRYGGDEFIIVLTEASENETLRTAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNG
                     HPDYERLIQIADEALYIAKRRGRNRVELWKASL"
     gene            609..2998
                     /gene="dosP"
                     /locus_tag="N7C74_002269"
                     /gene_synonym="pdeO"
                     /pseudo
     CDS             609..2998
                     /gene="dosP"
                     /locus_tag="N7C74_002269"
                     /gene_synonym="pdeO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
     gene            3256..3837
                     /gene="ddpX"
                     /locus_tag="N7C74_002270"
     CDS             3256..3837
                     /gene="ddpX"
                     /locus_tag="N7C74_002270"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HGU2432131.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            3851..5401
                     /locus_tag="N7C74_002271"
     CDS             3851..5401
                     /locus_tag="N7C74_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU2432132.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASNDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            5403..6425
                     /locus_tag="N7C74_002272"
     CDS             5403..6425
                     /locus_tag="N7C74_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2432133.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            6422..7318
                     /gene="ddpC"
                     /locus_tag="N7C74_002273"
     CDS             6422..7318
                     /gene="ddpC"
                     /locus_tag="N7C74_002273"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HGU2432134.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRTDAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQV
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            7315..8301
                     /locus_tag="N7C74_002274"
     CDS             7315..8301
                     /locus_tag="N7C74_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2432135.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDTVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARDSGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            8294..9220
                     /locus_tag="N7C74_002275"
     CDS             8294..9220
                     /locus_tag="N7C74_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HGU2432136.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMMFQDPLSSLNPRLPVWRI
                     ITEPLWIAKHSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     RERCPLATSGCEVRQSLTTRADGREIRCWRAL"
     gene            complement(9276..9707)
                     /gene="osmC"
                     /locus_tag="N7C74_002276"
     CDS             complement(9276..9707)
                     /gene="osmC"
                     /locus_tag="N7C74_002276"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HGU2432137.1"
                     /translation="MTIHKKGQAHWESDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            10052..10267
                     /gene="bdm"
                     /locus_tag="N7C74_002277"
     CDS             10052..10267
                     /gene="bdm"
                     /locus_tag="N7C74_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HGU2432138.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            10369..10506
                     /gene="sra"
                     /locus_tag="N7C74_002278"
     CDS             10369..10506
                     /gene="sra"
                     /locus_tag="N7C74_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HGU2432139.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            10663..12360
                     /gene="maeA"
                     /locus_tag="N7C74_002279"
     CDS             10663..12360
                     /gene="maeA"
                     /locus_tag="N7C74_002279"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HGU2432140.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDRDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            12494..13504
                     /gene="adhP"
                     /locus_tag="N7C74_002280"
     CDS             12494..13504
                     /gene="adhP"
                     /locus_tag="N7C74_002280"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HGU2432141.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            13650..13934
                     /locus_tag="N7C74_002281"
     CDS             13650..13934
                     /locus_tag="N7C74_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HGU2432142.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAAKTVDVSRLRRLVAQ"
     gene            complement(14162..14815)
                     /gene="fdnI"
                     /locus_tag="N7C74_002282"
     CDS             complement(14162..14815)
                     /gene="fdnI"
                     /locus_tag="N7C74_002282"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HGU2432143.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(14808..15692)
                     /gene="fdnH"
                     /locus_tag="N7C74_002283"
     CDS             complement(14808..15692)
                     /gene="fdnH"
                     /locus_tag="N7C74_002283"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HGU2432144.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQSGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(15705..18752)
                     /gene="fdnG"
                     /locus_tag="N7C74_002284"
     CDS             complement(15705..18752)
                     /gene="fdnG"
                     /locus_tag="N7C74_002284"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(18165..18167),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HGU2432145.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            18984..19865
                     /gene="yddG"
                     /locus_tag="N7C74_002285"
     CDS             18984..19865
                     /gene="yddG"
                     /locus_tag="N7C74_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HGU2432146.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         4813 a         5141 c         5101 g         4823 t
ORIGIN      
        1 aatttttatt acagataaga aataccgtat cgcaaattat taccttgctg cgtgaattgt
       61 ttgaagaagt atcgcgccac gaagtcggta tggatgtact gacgaaatta cttaaccgcc
      121 gtttcctgcc gactatcttc aaacgcgaaa ttgcccatgc caaccggacc ggtacaccgc
      181 tgtcagtgct gattattgac gttgataaat tcaaagagat caacgatacg tggggccata
      241 acactggcga tgaaattctg cgtaaagtct ctcaggcctt ttatgacaac gtccgcagta
      301 gtgattatgt tttccgctac gggggcgatg aatttatcat tgttttgact gaagcttctg
      361 aaaacgaaac gttacgtacc gcagaacgta ttcgcagtcg ggtggagaaa accaaactga
      421 aagccgcaaa cggcgaagat attgccctct cactttccat cggtgccgcc atgtttaatg
      481 gtcatcctga ctatgagcgc ctcattcaaa tagccgatga agctctgtat atcgccaaaa
      541 gacgaggtag aaaccgtgtt gaactctgga aagccagtct ttagatgcgc caggatgcag
      601 aggtaatcat gaagctaacc gatgcggata atgccgccga tggcattttt ttccccgccc
      661 ttgagcaaaa tatgatgggt gcggtgttaa ttaacgaaaa tgatgaagtg atgtttttca
      721 accccgccgc agagaagctc tgggggtaca aacgtgaaga agtcattggc aataacattg
      781 atatgctgat tccgcgggat ttgcgtcctg cgcatcctga atacattcgt cacaaccgtg
      841 aaggcggtaa agcgcgcgtc gaggggatga gtcgggagct gcagctggag aaaaaagacg
      901 gcagtaaaat ctggacccgt tttgcgctat cgaaagtgag cgccgagggg aaagtttatt
      961 acctggcgct ggtacgggat gccagcgtag aaatggcgca aaaagaacag accaggcaat
     1021 tgattattgc cgttgaccat ctcgaccgac cggtgattgt cctcgatccg gaacgccata
     1081 ttgtgcagtg caatcgcgca tttaccgaaa tgtttggtta ctgcattagc gaagccagcg
     1141 gtatgcagcc tgatacactc ctgaacattc ctgaattccc tgccgataac cgcattcgtt
     1201 tacaacagtt gctttggaaa accgcccgcg atcaggacga atttctgctg ttgacgcgca
     1261 ccggtgaaaa aatctggatt aaagcttcta tcagcccggt ttatgacgtg ctcgcgcatc
     1321 tgcagaacct ggtaatgacc ttctcggata tcaccgaaga acggcaaatc cgccagcttg
     1381 aaggcaatat tctcgccgcc atgtgcagca gcccgccatt tcatgaaatg ggggaaatca
     1441 tttgtcgtaa catcgaatct gttctcaacg aatcgcatgt ttcgctgttc gcactgcgca
     1501 acgggatgcc gatacactgg gcgtcatctt cgcacggtgc agaagttcaa aatgcgcaaa
     1561 gctggtcagc gaccattcgt cagcgtgatg gcgcgcctgc ggggatcctg caaattaaaa
     1621 cctcgtcagg agcagaaacc agcgccttta tcgaacgcgt ggcagatatc agccagcata
     1681 tggccgcgct ggcgctggaa caggaaaaaa gccgtcagca tattgaacaa ctcatccaat
     1741 ttgatccgat gaccggtctg ccaaatcgca ataacctgca caattacctc gatgacctgg
     1801 tcgacaaagc cgtctctcca gtatcggtgt tgaccatatt caggatgtga ttgatagcct
     1861 tggctatgcg tgggccgatc aggcattgct ggaagtggtc aatcgctttc gtgaaaaact
     1921 caaaccggat cagtatctct gtcgtatcga aggtacgcag tttgtcctcg tgagcctgga
     1981 aaacgacgtc agtaacatta cccagatcgc cgatgagcta cggaatgtgg tcagcaagcc
     2041 gataatgatt gacgataacc ccttcccgct taccttgagt attggcatca gctacgacgt
     2101 gggtaaaaat cgcgattacc tgctctccac tgcccataac gcgatggatt atattcgcaa
     2161 gaatggcggt aacggctggc agttcttcag cccggcgatg aacgaaatgg taaaagagcg
     2221 tttggtttta ggcgcagcgc tgaaagaagc gattagcaat aaccaactga aactggttta
     2281 ccagccgcaa atcttcgcag aaacgggtga actctacggc atcgaagccc ttgctcgctg
     2341 gcacgatccc cagcatggtc atgtgccccc ttcacggttt attcctctcg cagaagagat
     2401 tggtgaaatc gaaaatattg ggcgctgggt catcgcggaa gcttgccgtc agttagcaga
     2461 atggcgtagc cagaatattc atatcccggc gttatccgtg aacttgtcgg cgctgcactt
     2521 tcgcagtaat cagctgccta atcaggtgtc tgatgcaatg caagcctggg gtattgacgg
     2581 ccaccagctg acggtagaaa tcacggaaag catgatgatg gaacacgata ccgaaatctt
     2641 taagcgcatt cagatcctgc gtgatatggg cgtgggttta tcggtagatg attttggcac
     2701 gggcttttcc ggattatccc gcttagtcag tcttccggta acggaaatca aaattgacaa
     2761 aagttttgtc gatcgttgtc tgacggaaaa acgcatcctg gccttacttg aagccattac
     2821 cagcattggg caaagcctca atttaaccgt cgtggcggaa ggcgtcgaaa ccaaagagca
     2881 atttgagatg ctacgaaaga tccactgtcg cgttattcag ggatatttct tttcccgccc
     2941 cctacccgcc gaagaaattc caggctggat gagcagcgtg ttaccgctga aaatctgaca
     3001 aattcctctc tcccgcactc gcgggttttc atttaacgtg acactgtcac ttaaatactg
     3061 tgatttcagc tacgattccg ggagattcct tccttaactt cccctcttgc accaaaactg
     3121 cactacgctg cacaaatgtt gatcaaaagt taaaaaatat aactttaagc cagcattctg
     3181 gcgcttatcc cggcatggca tgagatctgc ataagcggaa agcgcagcaa tttttgtctt
     3241 atacaggaac cgtttatgtc ggataccacc gaactggttg atttagccgt gatcttccct
     3301 gatctggaga tcgaattgaa atacgcctgc gctgataaca tcacaggtaa agctatttac
     3361 cagcaagcgc gttgtctgtt acacaaggat gcgattaccg cgctggcgaa aagtatcagt
     3421 atcgcccagc tatcaggctt acaactggtg atttacgatg cgtatcgccc acaacaagca
     3481 caggcgatgt tgtggcaagc ctgcccagac ccgcaatatg ttgttgatgt gacggtcggt
     3541 tctaatcaca gccgtggcac ggcgatcgac ctgacgcttc gtgatgagca cgggaacatc
     3601 ctggatatgg gcgcagggtt cgatgaaatg cacgagcgct cccatgccta tcacccttcc
     3661 gtcccgcccg ccgctcagcg caatcgcctg ttgctgaatg cgataatgac tggtggcggt
     3721 tttgtcggta tctccagcga atggtggcac ttcgaattac cccaggcagc gagttaccct
     3781 ctgctcgctg atcaattcac ctgttttata tcccctggca cacagcacgt cagttaatca
     3841 ggaaactgcc atgaagagat cgatatcgtt tcgtcccaca ttgctcgcgc tcgtccttgc
     3901 cacaactttc ccggttgcgc acgccgccgt accgaaagat atgctggtga ttggtaaggc
     3961 cgccgatcca caaaccctcg acccggcggt aacaatagat aataacgact ggacagtgac
     4021 ctacccgtct tatcagcgac tggttcagta caaaacggac ggtgataaag gctcaaccga
     4081 cgttgaaggc gatctggcaa gtagctggaa agcgtctaac gatcaaaaag agtggacgtt
     4141 caccctgaaa gataatgcta aatttgccga tggcacacct gtcactgccg aagcagtaaa
     4201 actctctttt gagcggttac taaaaatcgg ccaggggcca gcagaagcat ttcccaaaga
     4261 tttaaagatt gatgctcccg acgaacatac ggtgaagttt acccttagcc agccatttgc
     4321 accgttcctc tacacgctgg cgaatgacgg tgcatccatt atcaatccgg cggtattaaa
     4381 ggaacatgcg gcggatgatg cccgcggctt cctcgcgcaa aataccgccg gttccggacc
     4441 atttatgctg aaaagctggc aaaaaggtca gcaattagtt ctggtgccaa atccgcatta
     4501 ccccggcaat aaaccgaact ttaagcgagt atcggtaaaa attatcggtg aaagtgcctc
     4561 ccgtcgcctg cagctctccc gtggcgacat tgacattgcc gatgcgctgc cggtggatca
     4621 actcaacgcc ctgaagcagg aaaacaaagt caatgtggca gagtatccgt cactgcgcgt
     4681 cacctatctg tatctgaata acagcaaagc gccacttaat caggcggatc tgcggcgggc
     4741 catttcctgg tctaccgatt atcagggcat ggttaacggc attctgagtg gtaacggaaa
     4801 acaaatgcgc ggcccgattc cggaaggcat gtggggctac gatgcgacgg caatgcaata
     4861 caaccatgac gaaacgaaag ccaaagccga atgggataaa gtgacgagca aacccaccag
     4921 cctgacgttt ctctactccg ataacgatcc gaactgggaa ccgatcgctc tggcaacaca
     4981 atccagcctc aacaagctgg gcatcaatgt gaagctggaa aagctggcga acgccaccat
     5041 gcgcgacaga gtgggtaaag gtgattacga cattgcgatt ggcaactgga gtccggattt
     5101 tgccgacccg tatatgttta tgaattactg gtttgagtca gacaaaaaag gtctgccggg
     5161 taaccgctcg ttctatgaaa acagtgaggt cgataagtta ctgcgcaatg cgcttgcgac
     5221 caccgaccag acgcagcgta cccgagacta ccagcaggca cagaaaatcg tcattgatga
     5281 cgctgcttat gtgtacctgt tccagaaaaa ctaccaactg gcgatgaaca aagaggtgaa
     5341 aggctttgtg ttcaatccca tgctggaaca ggtcttcaat atcaatacca tgagtaaata
     5401 acgtgacctt ctggagtatt ttacgccaac gctgctgggg gctggtgctc gtggtggcgg
     5461 gcgtctgcgt gattacgttt attatctcgc atctgatccc cggcgatccg gcgcggttac
     5521 tggcgggtga ccgcgccagc gatgctatcg tggaaaatat tcgccagcaa ctgggactgg
     5581 accagccact gtatgtacag ttttaccgct acgtcagcga cctgtttcat ggtgacctgg
     5641 gaacatccat tcgtactggg cgtccggtgc tggaagagtt gcgtatattt ttcccggcga
     5701 cgctggagct ggctttttgt gccctgctgc tggcactctt gattggcatc ccgctgggca
     5761 tactctctgc agtctggcga aatcgctggc tggatcatct ggtgcgaata atggcgatta
     5821 ccggaatctc cacacctgcg ttctggcttg ggctgggcgt catcgtgctg ttttatggtc
     5881 atctgcaaat acttcccggc ggcggaaggc ttgatgactg gctggatcca ccaacgcacg
     5941 ttaccggctt ttatctgctc gatgcgttgc ttgaaggcaa cggtgaagtc ttcttcaatg
     6001 cgttgcaaca tctcatctta ccggcattaa cgctggcgtt tgttcacctg gggattgtcg
     6061 cccgccagat ccgctcagcg atgctggaac aattgagtga agactacatt cgtaccgccc
     6121 gggccagcgg cttgcccggc tggtatatcg ttctatgtta tgcgctaccc aatgcgttga
     6181 tcccttcgat taccgtattg gggctggcgc tgggcgattt gttgtatggc gcagtgctca
     6241 ccgaaaccgt ttttgcctgg cccggaatgg gggcatgggt agtgacatca atacaggcgc
     6301 tcgacttccc ggcagtgatg ggctttgccg tcgtggtttc atttgcttat gtgctggtca
     6361 acctggtggt ggatttgctc tatttgtgga ttgatccgcg tatcggacgt ggaggtggtg
     6421 aatgatgcta agcgaggaaa cgtccgccgt acgcccacaa aaacaaacgc gatttaacgg
     6481 tgcaaaactg gtatggatgc tgaaaggcag tccgctcacc gtgaccggcg cagtcatcat
     6541 tgtattaatg ctactgatga tgattttttc accgtggctg gcgacgcatg atcccaacgc
     6601 cattgattta accgcccgcc ttttgccgcc ttctgcggcg cactggtttg gcaccgatga
     6661 agtgggacgc gatctgttca gccgcgtact ggtcggcagt cagcaatcaa ttctcgccgg
     6721 attagtcgtg gttgccattg cgggtatgat tggttcgcta ctcggatgtc tatccggtgt
     6781 gcttggcgga cgcacagacg ccatcatcat gcgcatcatg gacattatgc tgtcgattcc
     6841 ttcgttggta ctgacaatgg cactggctgc cgctctcggg ccgagtttgt ttaacgccat
     6901 gctggctatt gctattgtgc gaattccctt ttatgtgcgc ctggcgcggg ggcaagcatt
     6961 agttgtacgc cagtatacct atgttcaggc ggcgaaaacc tttggcgcgt ctcgttggca
     7021 tctgatcaac tggcatattt tacgtaactc cctaccgccg ctgatcgtgc aggtatcgct
     7081 ggatatcggt agcgcgattt taatggccgc cacgttggga tttattggcc tgggtgctca
     7141 acaaccgagt gctgaatggg gcgcgatggt agcaaatggt cgcaactatg tactcgatca
     7201 gtggtggtat tgcgcatttc cgggggcagc gattttgctt accgccgtcg ggtttaatct
     7261 ctttggtgat ggtattcgcg atctgcttga cccgaaagca ggaggaaagc agtcatgacc
     7321 caacccgttc tggacattca acaactgcat ttgagtttcc ccggttttaa cggcgacgtt
     7381 cacgcgctca acaatgtgtc cttgcagatt aaccgcggtg aaattgtcgg tctggtggga
     7441 gaatccggct caggtaaatc agtcaccgca atgctgatta tgcgtctgct accgacgggc
     7501 agttattgcg tacatcgggg gcagatttca ctgctgggag acgatgttct taacgcgcgg
     7561 gaaaaacagc ttcgtcagtg gcgcggcgca cgagtggcga tgatctttca ggaaccgatg
     7621 accgccctca atccgacacg tcgaataggt cttcagatga tggacgtgat ccgccatcat
     7681 caaccaataa gtcgtcggga agccagagct aaagcgattg ccctgctgga agagatgcaa
     7741 atcccggata ccgtggaagt tatgtcgcgc tatccgtttg agctttcagg tggtatgcgc
     7801 cagcgggtaa tgattgcgct ggcattctcc tgcgaaccgc aattgattat tgccgacgaa
     7861 ccgactacgg cgctggacgt cacggtacag ttgcaggtac tgcgtttgct taaacataaa
     7921 gctcgcgaca gtggaactgc ggtactgttc atcagccatg atatggccgt ggtgtcgcaa
     7981 ctgtgcgata gcgtttacgt gatgtatgcc ggaagcgtga ttgagagcgg cgtgacggca
     8041 gacgttatcc atcatccccg gcatccgtat accattggtt tgctgcaatg cgcaccagaa
     8101 catggagtac cacgccagcc attacccgcc attccaggga cggtaccaaa tctcacccat
     8161 ttgcctgacg gctgcgcttt tcgcgatcgt tgctatgcgg caggtgcaca gtgtgaaaac
     8221 gtcccggcgc tgacagcgtg tggtgacaac aaccagcgct gcgcctgttg gtatccgcag
     8281 caggaggtca taagtgtctg acacgttatt aacgttacgc gacgtccata tcaatttccc
     8341 ggcgcgtaaa aactggcttg gtaaaactac ggaacatgtt catgccatta atggtattga
     8401 tttacagatc cgccgtggtg aaaccttagg gatcgtcggc gagtcaggct gcggcaaaag
     8461 caccctcgca cagcttttaa tgggtatgct gcaaccgagc cacgggcagt acatccgttc
     8521 tggctcacaa cgcattatgc agatgatgtt tcaggacccg ctctcttcgc ttaatccgcg
     8581 cttaccggtg tggcgcatca tcacagaacc gctctggata gctaagcata gtagtgaaca
     8641 acagcggcga gcgttggcag aggagctggc tgtgcaggtg ggtattcgtc cggagtatct
     8701 cgaccgcctg cctcatgcgt tctccggcgg gcagcggcaa cgcatcgcca ttgccagagc
     8761 actctcttcg cagcctgacg tgattgtgct tgatgagcca acctctgcgc tggatatctc
     8821 cgtgcaggcg cagatcctca atttactggt aacgctacag gaaaatcacg ggctgactta
     8881 tgtgctgatt tcacacaatg tctcggtgat acgtcatatg agcgatcggg tggcggtgat
     8941 gtatctcggg cagattgtgg aattagggga agcgcaacag gtgctaacgg ctccggcaca
     9001 tccatacacc cgattattgc tggattccct ccccgccatt gataaaccac tggaggaaga
     9061 atgggcatta cgtaaaacgg atctgccagg aaaccgcacg ttgccacagg gctgtttttt
     9121 ccgcgaacgt tgcccccttg caaccagcgg atgcgaagtc cggcaatcat taacgacgcg
     9181 ggctgacgga cgtgagatcc gttgctggcg ggcgctgtag agatgctaac aacgcatcag
     9241 gcattcatgc gcctgacgcg tcatccggca atgctttacg atttcaactg gtaatccagc
     9301 gtaatttccg ctttcagtac ctgagagacc gggcatcctg cttttgcttt ctggatgatg
     9361 ccgtcaaagg tagaggcatc aatacccggc accgcaactt cactcttcag tgcgattttc
     9421 gtaatcgcaa aaccggcatc cactttatcc agcgacacat cggcggtggt atcaatcgat
     9481 gttggcgtga atcccgcttc ccccagcatt aatgaaagcg ccattgagaa acatgcggca
     9541 tgcgctgcgc caatcagttc ttcagggttg gttccttttt cgccttcaaa acgcgtgtta
     9601 aatccatacg gctgttggtt cagcacgcca ctctcggtgg atactgttcc cttcccgcgt
     9661 ttgatatcgc tttcccagtg tgcctgacct ttcttatgga ttgtcattgt tgctctcctg
     9721 tgggcttaaa aaggaaatta tagactacgt gagaaaccgc acgaggataa gcaggatatt
     9781 ccgaataaaa tacggcaaaa atattacttc tgcacttaaa ttaaattccc acaatagata
     9841 accggaaata tatatctcaa gagagattgc atcaatatta atcaactcat cacaataata
     9901 atacctatca atatcatggg gttaaaatat aaaaatcatc aaccaggact aatcttaaca
     9961 acgaagcggc aaatatttgc catgctgaat gtgctttata aatctgcgat ccgtagcaga
    10021 cactataaat acacagacac ggagaatcac tatgtttact tattatcagg cagaaaattc
    10081 aacagcagaa cccgctctgg ttaatgccat tgagcaaggc cttcgggcag agcacggtgt
    10141 tgtcactgaa gatgacattc tcatggagct gaccaaatgg gtggaagctt ctgataacga
    10201 catcctcagt gatatctacc agcaaacgat caattatgtg gtcagtggcc agcaccctac
    10261 gctttaagat gctatgcttg atcggcaacc taatttaggg gtttagcacg tgtttcttcg
    10321 ctacggcgat gttgtcctta aaactagcaa caggattgag gagttaaaat gaaatcgaac
    10381 cgtcaggcac gtcatattct tggactggac cataaaatat ctaaccagcg caaaatagtt
    10441 accgaaggtg acaaatccag cgtggtaaat aacccaaccg gcagaaaacg ccccgctgaa
    10501 aagtaattca taaccatcag tcctcaatga cgattaaaca ccattgcctg cgcaatggtg
    10561 tttttgtttt tatctgcttt atacttgggg ccgacgccct ggcggtaaag caaagacgat
    10621 aaaagccccc cagggatgga tattcaaaaa agagtgagtg acatggaacc aaaaacaaaa
    10681 aaacagcgtt cgctttatat cccttacgct ggccctgtac tgctggaatt tccgttgttg
    10741 aataaaggca gtgccttcag catggaagaa cgccgtaact tcaacctgct ggggttactg
    10801 ccggaagtgg tcgaaaccat cgaagaacaa gcggaacgag catggatcca gtatcaggga
    10861 ttcaaaaccg aaatcgacaa acacatctac ctgcgtaaca tccaggacac caacgaaacc
    10921 ctcttctacc gtctggtaaa caatcatctt gatgagatga tgcctgttat ttatacccca
    10981 accgtcggcg cagcctgtga gcgtttttct gagatctacc gccgttcacg cggcgtgttt
    11041 atctcttacc agaaccgcca caatatggac gatattctgc aaaacgtgcc gaaccataat
    11101 attaaagtga ttgtggtgac tgacggtgaa cgtattctgg ggcttggtga ccagggcatc
    11161 ggcgggatgg gcattccgat cggtaaactg tcgctctata ccgcctgtgg cggcatcagc
    11221 ccggcgtata cccttccggt ggtgctggat gtcggaacga acaaccaaca gctgcttaac
    11281 gatccgctgt atatgggctg gcgtaatccg cgtatcactg acgacgaata ctatgaattc
    11341 gttgatgaat ttatccaggc tgtgaaacaa cgctggccag acgtgctgtt gcagtttgaa
    11401 gactttgctc aaaaaaatgc gatgccgtta cttaaccgct atcgcaatga aatttgttcc
    11461 tttaacgatg acattcaggg caccgcggcg gtaacagtcg gcaccctgat cgcagcaagc
    11521 cgcgcggcag gtggtcagtt aagcgagaaa aaaatcgtct tccttggcgc aggttcagcg
    11581 ggatgcggca ttgccgaaat gatcatcgcc cagacccagc gcgaaggatt aagcgaggaa
    11641 gcggcgcggc agaaagtctt tatggtcgat cgctttggcc tgctgaccga caagatgccg
    11701 aacctgctgc ctttccagac caaactggtg cagaagcgcg aaaacctcag tgacagggat
    11761 accgacagcg atgtgctgtc actgctggat gtggtgcgca atgtaaaacc agatattctg
    11821 atcggcgtct caggacagac cgggctgttt acggaagaga tcatccgtga gatgcataaa
    11881 cactgtccgc gtccgatcgt gatgccgctg tccaacccga cgtcacgcgt ggaagccaca
    11941 ccgcaggaca ttatcgcctg gactgaaggt aacgcgctgg tcgccacggg cagtccgttt
    12001 aatccagtgg tatggaaaga taaaatctac cctatcgccc agtgtaacaa cgcctttatt
    12061 ttcccgggca tcgggctggg tgttattgct tccggcgcgt cacgtatcac cgatgagatg
    12121 ctgatgtcgg caagtgaaac gcttgcccag tattcgccgc tggtcctgaa cggcgaaggt
    12181 ctggtactac cggaactaaa agatattcag aaagtctccc gcgcaattgc gtttgcggtt
    12241 ggcaaaatgg cgcagcagca aggcgtggcg gtgaaaacgt ctgccgaagc tttgcaacaa
    12301 gccattgacg ataatttctg gcaagccgaa taccgcgact accgccgtac ctccatctaa
    12361 gcctgcgccc ggtagtgaag gctaccgggc tatttccctc tccctttttc agatctcatc
    12421 catactgggt agtggcgaat aaatctcatt tgcctcacct gctatgcaga acatcatccg
    12481 aaaaggagga actatgaagg ctgcagttgt tacgaaggat catcatgttg acgttacgga
    12541 taaaacactg cgctcactga aacatggcga agccctgctg aaaatggagt gttgtggtgt
    12601 atgtcatacc gatcttcatg ttaagaatgg cgattttggt gacaaaaccg gcgtaattct
    12661 gggccatgaa gggattggtg tggtggcaga agtgggtcca ggtgtcacct cattaaaacc
    12721 aggcgatcgt gccagcgtgg cgtggttcta cgaaggatgc ggtcattgcg aatactgtaa
    12781 cagtggtaac gaaacgctct gccgttcagt taaaaatgcc ggatacagcg ttgatggcgg
    12841 gatggcggaa gagtgcatcg tggtcgccga ttacgcggta aaagtgccag atggtctgga
    12901 ctcggcggcg gccagcagca ttacctgtgc gggggtcacc acctacaaag ccgttaagct
    12961 gtcaaaaatt cgtcccgggc agtggattgc tatctacggt cttggcggtc tgggtaacct
    13021 cgccctgcaa tacgcgaaga atgtctttaa cgcgaaagtg atcgccattg atgtcaatga
    13081 tgagcagtta aaactggcaa ccgaaatggg tgcagattta gcgattaact cacgcaccga
    13141 agacgccgcc aaaattgtgc aggagaaaac cggtggcgct cacgctgcgg tggtaacagc
    13201 agtagctaaa gctgcgttta actcggcagt tgatgctgtc cgtgcaggcg gtcgtgttgt
    13261 ggctgtcggt ctgccgccgg agtctatgag cctggatatc ccacgtcttg tgctggatgg
    13321 cattgaggtg gtcggttcgc tggtcggcac gcgccaggat ctaactgaag ccttccagtt
    13381 tgccgccgaa ggtaaagtgg tgccgaaagt cgccctgcgt ccgttagcgg acatcaacac
    13441 catctttacc gagatggaag aaggcaaaat ccgtggccgt atggtgattg atttccgccg
    13501 ctaagaggcc tttgctgcga ctcccatgtt cgggtcgcag catcgcacac tctccaacat
    13561 gaaatggcta aatggattat tgttgcgtag gaaggaacaa taatgatagt gttcccagag
    13621 aaggaacgct actgagagta aagacactaa tgaaaatggc caatcatccc cgcccggggg
    13681 acattattca ggaatcactg gacgaactta atgtcagcct gcgcgagttt gccagagcaa
    13741 tggaaattgc tccctcaacg gcaagccgat tgctgaccgg aaaagcagct ttgacgccag
    13801 aaatggcaat taaactttcc gtggtgatcg gcagttcgcc gcaaatgtgg ctgaatctgc
    13861 aaaacgcctg gagtctggca gaggctgcaa aaacggtgga tgtgtcgcga ttacgccgtc
    13921 tggttgcgca ataaaaaatg gcgcctttta cagcgccatt tgaaactgat gacaaacgca
    13981 acattgcctg atgcgctacg cttatcaggc ctacacgttc actgcaatct attggatttg
    14041 aacggtttgt aggccggata aggcgttcac gccgcatccg gcatgaacaa agcctacgtt
    14101 gtctacaata tgaaaatggc gccttttaca gcgccatttt cagcttaaag ttcaaaagag
    14161 attatatccc ttcttcactc tcttttttcg cttctgcctt ctcgatttca cgataccagc
    14221 gcggatggtg tttcttcgcc cagcgacggc tcaccttccc ttcgatcatc cctttaatcg
    14281 atcctttcac ccagaatgcc atatacatat ggatcaggat ggcgtggatc aggatgatac
    14341 ccgcagccgc gtggatcagc aggctgtagc gaacaacctg catcgggaag tactgcgcaa
    14401 agtacggacg ccagataatc accccggtca ccagcagcac gaaaatcatg ctcatgatcg
    14461 accagaacat catcttttgc ccggcgttgt acttaccgac atccgccact ttatgctcat
    14521 tgcctttcaa tacttcgaca atgtttaaca gccacggaat atctttctta tccgggatgt
    14581 tgtgatgcac aaaacgcaca aacataaaca tcagtgcgac gaaaatcgca atgccgaaga
    14641 atgggtgcaa aatgcgtccc atctgcggcg taccgaaggt ttgcgtcagc cattgcagcg
    14701 tcgggaagaa gaacgaaatc ccggacagcg ccaccaggaa gaagcaaatc accacggtcc
    14761 agtgacaggc gcgatcaata aatttggtgc gcacaatcat tttcgactta ctcatgatga
    14821 tcctcctcgt catcgtccac ttccttattc gggccaatac cgatgtagtg gaaaatcaac
    14881 cctgcaaaag tggcaataaa gccagccgct gccagcggtt tcaacgcgcc tttccacagg
    14941 cttaccgagg tgtcgatctt cggatctttc ggcagaccgt gatacagctc cggctgatcg
    15001 gcgtgatgca gcacgtacat aacgtgcgta ccaccgaccc cttccgggtt gtagacgcca
    15061 gcatgttcgt aaccacgcgc tttcagtttc gccacgcgct gttccgccag ctccagcatc
    15121 tccttcttgg tgccgaagtg gatagccccg gtcggacagg ttttcacaca agccggttcc
    15181 tggccgacgc tgacgcgatc gacgcagagc gtgcatttat atacccggtt atcctctttg
    15241 ttgaggcgcg gaatattaaa cggacacccg gcaatgcagt aaccacagcc gatgcagttt
    15301 tccgactgga aatcgacaat cccgttagcg tactgaatga ttgcaccggc agacgggcac
    15361 gccttcaggc agcccggatc ttcacagtgc atacagccat ctttacggat cagccactcc
    15421 agcttgccgc tctgttcggt ttcgctaaag cgcatcaccg tccaggactt ggcgctcaga
    15481 tcggcggggt tatcgtaaac cccgacgcag tgccccactt catcacggat gtcgttccac
    15541 tccgaacacg ccacctgaca ggctttacag ccgatacagg tggaaacgtc gataagtttt
    15601 gcgacttctg ctttgtaatc acgcacctga gaaggcggcg tgatggagtt agttgcggac
    15661 cttttgataa tgtcctgcgt ttccatagcc atctgttcgc ccccttacgc cttctcgatg
    15721 ttgactaaga acgctttata ttccggcgtt tgcgagtttg catcaccgac attcggcgtc
    15781 agagtgttag ctatataacc tttacgcgcg acaccctcaa agccccagtg gattggaata
    15841 cccaccgttt caacctgctg accatttaca ttcagcggtt tcagacgacg cgttaccaca
    15901 gccaccgcgc ggataaagcc acgcttgctt gagacagtga cacgatcgcc attattaatg
    15961 cctttcgccg ccgccagcgt ttcgctgatt tccacaaact gttccggctg agcaattgcg
    16021 ttgagcaacg cgtgcttggt ccaggtgtgg aagtgctcgg tcagacgata ggtcgtaccc
    16081 acatacggga actgctcttt tttacccatc cgcagcgcgt cttgttcata cagacgaacc
    16141 accgggttag aaaccacgtt cggatgcagc gggttagttc ccagcggcgt ttcaattggc
    16201 tcgtagtgtt ccgggaacgg accttccgcc attttgttga tggcaaacag gcgtcccatc
    16261 ccttccggct gcatgataaa cggcccggtt ggcgtacccg gtgcggcatt gccgaagtca
    16321 gggatatcgt tacccgtcca cttgctgccg ttccactgga tcagcatccg tttcggatcc
    16381 cacggtttac cgttgatatc tgccgaagca cggttgtaga gtacgcgacg gttaagcggc
    16441 cacgcccagg cccatcccag cgtattcccc agaccggacg ggtcggagtt atcgcggtta
    16501 gccatctggt tgccctgctc tgtccagcta ccggtgtaga tccagcaaga agatgcggtt
    16561 gtaccgtcat cacgcagatg cgcaaagcta ctcagcaact gacctttctt cgcaatcagc
    16621 acgccattag catcatagag atcttccagc gcatagccgt tgttctcttt agctacttcg
    16681 tcagattgcg gttcgtgcgg ctgcttgtag ttccagctca tcttcatcag cggttctacg
    16741 cctttaccac cttcagcctg gtacagctcg cgcagatgat ggtagatacc cgccagaatt
    16801 tcgccgtcgt tacgcgcttc gcccggcgcg tcctgacctt tccagtgcca ctgcaaccag
    16861 cgaccggagt tagcgataga accatcttct tctgcaaagc aggtcgaagg cagacggaat
    16921 acttcagtct gaatagacgc cggatcgaca tcgttcgact cgccgtggtt ctgccagaag
    16981 gtagaggttt cagtcaccag cggatcgata accaccatgt acttcagctt gctcaggcag
    17041 ctcaccactt tgtttttgtc cgggaaggac gcaaccgggt taaagccctg gcagaaataa
    17101 ccggtgactt tgccttcatc catcatgttg aaatacttga tgacgtcgta ggtctggtcc
    17161 cacttcggca gccagtcata gccccagttg ttctctttct gcgcggcatc gccatagaaa
    17221 gatttcatca ggctaacgaa gaacttcgga tagttgctcc agtagttcac ctgaccagcc
    17281 agcgtcgctt tcggcgtgtt cgcttccaga tacgactgca aatcaacctg tttttctgac
    17341 ggcagcgtca gataacctgg caggctggta gagagcagac ctaagtcagt cagaccctga
    17401 atgttggagt gaccacgcaa tgcattcacg ccgccaccgg ccatacccat gttaccgagc
    17461 agcaactgga tcatcgccat agtacggatg ttctgcgcac ccacggtgtg ctgcgtccag
    17521 cccagcgcgt acaggaaggt ggttgtgcga tccggtgcgc tggtggaggc cagcacttca
    17581 cacactttca ggaagtcggc ttttggcgta ccacagatgt tttcaacaac gtccggcgtg
    17641 tagcgggaaa cgtgctcttt cagcaggttc cacacacagc gcggatgagt cagtgtttca
    17701 tcgcgttttg catagccgtt ttcatcgaac tgatagttcc aggacgactt atcgtactgg
    17761 cgtttttcag cgtcgtagcc gctgaacaga ccgtcttcga aagcaaaatc atcacgcacc
    17821 agcaggctgg cgttggtgta atgcttaacg tattcggcgt tgattttgtt gttttcgatc
    17881 aggtagcgca aaacgccaga caggaacgta atgtccgtac cggaacgaat aggcgcgtag
    17941 atatccgcca cagaagcggt acgcgtaaaa cggggatcga caacgatcaa ggttgcatcg
    18001 ttgttgtttt tcgcttccat cgcccagcgg aaaccgacgg gatgcgcttc agcagcgtta
    18061 ccgcccatta ccattacgac gttagcgttt ttgatatcca cccagtggtt ggtcatcgca
    18121 ccgcgaccaa atgttggagc aagacttgct accgttggtc cgtgtcagac gcgcgcctgg
    18181 ttgtctaccg ccagcatccc gagggagcgg gcaaatttct gggtcagcat cccggtttcg
    18241 ttgctggcac cggaagcaca cagcataccg gtagaaagcc aacggtttac cgttacgccc
    18301 tgctcgttct tttcaataaa gttagcgtca cggtcagctt tcatcagctt cgcaatacgg
    18361 gagaatgctt cttcccagct aatgcgctgc catttgtcag aacctggcgc acgatattcc
    18421 gggtagcgca gacggttttc gctgttgacg taatccagca aaccggcccc tttcgggcac
    18481 agcgccccac ggcttaccgg atgatccggg tcaccttcaa tgtgataaat cgcttctctg
    18541 gcgtttttcg cgccatcacc caggctatac atcaatagcc cgcaacctac ggaacagtat
    18601 gtgcaggtgt tacggatctc tttagcgcgt aataatttgt agtttcgcgc ctgagccagt
    18661 gcttgcttcg gggcaaagcc caatgccgct actgttgttc cagccatacc gcccgcgcag
    18721 attttaaaaa attgtctgcg actgacgtcc attgctttcc tcttttttca ggggtattac
    18781 tgcgcgagga aactaacagt aaaattctga atctttttgc gcgaaatcaa aaagaacacg
    18841 tgtttgccac aaaataatcc gcctgcgcaa attgacctac ctcaatagcg gtagaaaaac
    18901 gcaccactgc ctgacaggcc agttaaaaaa atgctataaa attcagctta atttttaacg
    18961 gcaagagaga caaaacagcg agcatgacac gacaaaaagc aacgctcata gggctaatag
    19021 cgatcgtcct gtggagcacg atggtaggat tgattcgcgg tgtcagtgag gggctcggcc
    19081 cggtcggtgg cgcagctgct atctattcat taagcgggct gctgttaatc ttcacggttg
    19141 gatttccgcg tattcggcaa atcccgaaag gctatttact cgccgggagt ctgttattcg
    19201 tcagctatga aatctgtctg gcgctttcct tagggtatgc ggcgacccgt catcaggcga
    19261 ttgaagtggg tatggtgaac tatctgtggc ccagcctgac aattctcttt gccattctgt
    19321 ttaatggtca gaaaaccaac tggctgattg taccaggatt attattagcc ctcgtcggcg
    19381 tctgctgggt gttaggcggt gacaatgggt tacattatga tgaaatcatc aataatatca
    19441 ccaccagccc attgagttat ttcctggcgt tcattggtgc gtttatctgg gcagcctact
    19501 gcacagtaac gaataaatac gcacgcggat ttaacggaat taccgttttt gtcctgctaa
    19561 cgggagcaag tctgtgggtt tactattttc tcacgccaca accagaaatg gtatttagca
    19621 cgccagtcat gattaaactc atctctgcgg catttacctt aggatttgct tatgctgcat
    19681 ggaatgtcgg tatactgcat ggcaacgtca ccataatggc ggtaggttcg tattttacgc
    19741 ccgtactttc ctcagcgctt gcagccgtgc tgctcagcgc cccgctgtcg ttctcgttct
    19801 ggcaaggcgc gctgatggtc tgcggcggtt ccctgctctg ctggctggcg acacgtcgtg
    19861 gctaaaataa ttgccggg
//
LOCUS       DBFIFM010000073        19825 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.073, whole genome shotgun sequence.
ACCESSION   DBFIFM010000073 DBFIFM010000000
VERSION     DBFIFM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.073"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..399
                     /locus_tag="N7C74_002286"
     CDS             <1..399
                     /locus_tag="N7C74_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HGU2432147.1"
                     /translation="TKQETKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVI
                     GHNVGSGSNSGTTAGAVGGGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKE
                     CQFTTGLAVVITTTYNETRIQPNTKCPEKS"
     gene            415..954
                     /locus_tag="N7C74_002287"
     CDS             415..954
                     /locus_tag="N7C74_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HGU2432148.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1047..2624)
                     /gene="guaA"
                     /locus_tag="N7C74_002288"
     CDS             complement(1047..2624)
                     /gene="guaA"
                     /locus_tag="N7C74_002288"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HGU2432149.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2693..4159)
                     /gene="guaB"
                     /locus_tag="N7C74_002289"
     CDS             complement(2693..4159)
                     /gene="guaB"
                     /locus_tag="N7C74_002289"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HGU2432150.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4321..5691
                     /gene="xseA"
                     /locus_tag="N7C74_002290"
     CDS             4321..5691
                     /gene="xseA"
                     /locus_tag="N7C74_002290"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HGU2432151.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQCNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGKVLKKVKQVKAGEMLTTRLEDGWVESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5688..5903)
                     /locus_tag="N7C74_002291"
     CDS             complement(5688..5903)
                     /locus_tag="N7C74_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGU2432152.1"
                     /translation="MELHCPQCQHVLDQDNGHAYCPSCGKVIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(5972..7444)
                     /gene="der"
                     /locus_tag="N7C74_002292"
     CDS             complement(5972..7444)
                     /gene="der"
                     /locus_tag="N7C74_002292"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HGU2432153.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7562..8740)
                     /gene="bamB"
                     /locus_tag="N7C74_002293"
     CDS             complement(7562..8740)
                     /gene="bamB"
                     /locus_tag="N7C74_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HGU2432154.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPAVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8751..9371)
                     /gene="yfgM"
                     /locus_tag="N7C74_002294"
     CDS             complement(8751..9371)
                     /gene="yfgM"
                     /locus_tag="N7C74_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HGU2432155.1"
                     /translation="MEIYENENDQVEAIKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9389..10663)
                     /gene="hisS"
                     /locus_tag="N7C74_002295"
     CDS             complement(9389..10663)
                     /gene="hisS"
                     /locus_tag="N7C74_002295"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HGU2432156.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10774..11892)
                     /gene="ispG"
                     /locus_tag="N7C74_002296"
                     /gene_synonym="gcpE"
     CDS             complement(10774..11892)
                     /gene="ispG"
                     /locus_tag="N7C74_002296"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HGU2432157.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(11919..12932)
                     /gene="rodZ"
                     /locus_tag="N7C74_002297"
     CDS             complement(11919..12932)
                     /gene="rodZ"
                     /locus_tag="N7C74_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HGU2432158.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPVVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13217..14371)
                     /locus_tag="N7C74_002298"
     CDS             complement(13217..14371)
                     /locus_tag="N7C74_002298"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HGU2432159.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPAAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14521..14952)
                     /gene="ndk"
                     /locus_tag="N7C74_002299"
     CDS             complement(14521..14952)
                     /gene="ndk"
                     /locus_tag="N7C74_002299"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HGU2432160.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15093..15947)
                     /locus_tag="N7C74_002300"
     CDS             complement(15093..15947)
                     /locus_tag="N7C74_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGU2432161.1"
                     /translation="MLSHNITIGAECARQRFRFATCHACVEACPAQALTLTEEGIAVD
                     AEQCIDCAVCQFICPQEAIRGVNSPLRYFCNHTLVAPFTTIAPTLEELLLWHKEQGIT
                     AIAIDLSQAKAWERALLLLNECLRQYGEEGWSVVSPHTSEVNVSRRRLFHVQRADVQM
                     TQIQPGLRRLHYLWPQISDARPMMDPQKCQLCGACWRACEQQVFSLNEGHLQINDALC
                     NGCQNCIAVCFHQAMTVELTILPAKIVNLHANRKVCKTCQKSFLTFQQNAQNCLYCQR
                     HRYGMRTP"
     gene            complement(15947..16768)
                     /locus_tag="N7C74_002301"
     CDS             complement(15947..16768)
                     /locus_tag="N7C74_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HGU2432162.1"
                     /translation="MHELPLMAFTLLLQASVGMTLWLAFLHRHILITSSARPVMRPPL
                     LLAFLAGAVGLLISTLHLGYPLNAMHALSHFSSSWLSREIIFGALYLALLGLSTLLVM
                     LRKGGWQLLLMLAAVVGIVDVFCMAQVYIHTSIITWQHYNTLIMFLGTVGILGSALVA
                     VFRISGILPQIDALRNGCVLVIALLVLLRLLVQPLWIGDLTANAMQIATLPHAPLAML
                     GQLKPILTLSWGISVIGMMFFAVGGCKKNIPAALFGSVMLVGSEVMLRFVFFSIG"
     gene            complement(16761..17390)
                     /locus_tag="N7C74_002302"
     CDS             complement(16761..17390)
                     /locus_tag="N7C74_002302"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex B
                     subunit"
                     /protein_id="HGU2432163.1"
                     /translation="MSQFKEFASVSDKQLGFFIDSSRCSGCKACQVACKDKNNLEVGR
                     RFRRVYEVNGGGFIATGEGGVSHNVFAYTLSVSCNHCADPICTKNCPTMAMHKRPGDG
                     IVRVNTDKCVGCGYCAWSCPYGAPQMNEQTGQMSKCDFCIDLQAKGEQPICVATCPLG
                     AIKFGPIDELREKYGDVSDVKGLPDSSITQPNLVIKPHQGAEKEASHHA"
     gene            complement(17387..19768)
                     /locus_tag="N7C74_002303"
     CDS             complement(17387..19768)
                     /locus_tag="N7C74_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
                     /protein_id="HGU2432164.1"
                     /translation="MDTPDEKERITFPQVSRRSFLQATSALITLPFISSTAKAQSPDA
                     SPEVTAPVADKVVPTCSTFDCGGKCDIRAHMRDGVVTQITTLPDNELDPQMPIMRACV
                     RGRGYRKFVYHPDRLKYPMKRVGKRGEGKFERISWDEATTLIADNLKRITQQYGPASR
                     YVHVGTAVSGGTFSGDAMARRLLNLTGGYLEYYHSVSLGNTAAATPYTYGVAASGNSM
                     DTLLDTKLVILWGHNPTETIFGHTNYYFQKMKQNGTRFIVVDPRYSDTVSSLADQWIP
                     LLPTTDNALMDAMMYVIISENLHDKTFIDTYTLGFDENSMPEGVPANESLVAYLFGAK
                     DGIHKTPEWAEKITHVPAQSIRQLARDYATTKPAALIQGWGPQRHICGERTARGSTLL
                     ASITGNVGIKGGWAAGYGGSSNRKFCVGPDMPENPVQAKISIMNWMQAADDASKVTPQ
                     DGLKGVDKLDSNIRLLFSLAGNYLANQNPDVHQAAKLLEDESKIEFIVLSDLFMTPSA
                     KYADVLLPETSFMERWNIGETWGTASYLILSEKPIEPDFERRTDYDWLRDVAKKLGVE
                     AEFSQGRDEKQWIEHIWEQTRLAMPDENLPDFATLQKTRRHLFKSAPHIAFEANIRDP
                     QNNPFPTPSGKIEIFSKRLFDMQDPEIPALSHYVPAFEGPEDKLTAKYPLQLITWKGK
                     NRANSTQYANPWLQEVQTQKLWLNPQDAKQRGISEGDSVKIYNDRGVSIIPVEITPRI
                     IPGVVAMQAGAWWQPDAQGIDRGGCANVLSSTRITALAKGNSHQTMLVEVEKA"
BASE COUNT         4904 a         5387 c         4823 g         4711 t
ORIGIN      
        1 accaaacaag aaactaaaac cgttaatatt atttccattc ttcccgcaaa agttgccgta
       61 gacaactccc aaaataaacg gaacgcacaa gccttcggcg cgcttattgg cgcagtcgct
      121 ggcggtgtta tcggccacaa cgtcgggtct ggcagcaatt ccggaacgac ggcaggtgca
      181 gttggcggcg gagctgtagg cgcggcagcg ggttctatgg tgaatgataa aaccttagtg
      241 gaaggtgttt ctttaacgta taaggaaggc accaaagtgt atacctctac tcaggtgggt
      301 aaagagtgcc agtttacgac aggtttagcc gttgttatta ccacgacgta taacgaaacg
      361 cgtattcagc caaataccaa atgtcctgaa aagagctaat aatcaggagg agtcatgaag
      421 aaagtttttc tttgcgccat cttagcctcc ttaagctatc cggctatcgc ctcatcattg
      481 caggatcaac tctctgctgt cgcagaagcg gaacagcaag gtaaaaatga agagcaaagg
      541 cagcatgacg aatgggtcgc ggagcgcaac agggaaatcc agcaagagaa gcaacgtcgc
      601 gcaaatgccc aggctgccgc taacaaaaga gcggcaacgg cagcggcaaa taagaaagct
      661 cgtcaggata aactggacgc cgaagccact gcggacaaaa aacgcgatca aagttatgaa
      721 gatgagctac gcagcttaga gattcagaaa caaaaactgg cgctggcaaa agaagaagcc
      781 cgcgttaagc gagaaaacga atttatcgat caggagctga agcacaaagc cgcgcaaact
      841 gatgtcgtgc aatctgaagc tgacgccaac agaaatatga ctgaaggcgg tcgcgatctg
      901 atgaaaagcg tgggcaaagc agaagagaat aaatcggaca gctggtttaa ttaatcgatg
      961 ttagtaactt caagcctata attcttgaag ataaaaaacc ctctgtagta acagagggtt
     1021 ttgttcattc atagtgcagg gtcaaatcat tcccactcaa tggtagctgg tggcttgccg
     1081 ctgatgtcat acaccacgcg ggaaataccg ttcacttcat tgataatgcg gttggaaacg
     1141 cgaccgagga aatcgtacgg cagatgcgcc cagtgtgcgg tcataaagtc gatggtttcg
     1201 acagcacgca gagagacaac ccagtcatac ttacgaccat cgcccattac gccaacggaa
     1261 cgtactggca ggaagacggt gaacgcctgg ctgactttgt cgtacaggtc cgctttacgc
     1321 agttcttcaa tgaagatggc gtcagcacgg cgcagcaggt cacagtactc tttcttcact
     1381 tcacccagaa cacgaacgcc aaggcctggt cccgggaacg ggtgacggta cagcatgtcg
     1441 tacggcaggc ccagctccag accaatctta cgcacttcgt ctttgaacag ctctttcagc
     1501 ggttcaacca ggcccatctt catctctttc ggcaggccgc ccacgttgtg gtgagatttg
     1561 atgacgtgtg ctttaccggt tgcagacgcc gcagattcga taacgtcagg gtagatggtg
     1621 ccctgcgcca gccacttcac gtcttccagt ttcagcgctt cttcatcgaa tacttcaacg
     1681 aaaacgcgac cgatgatttt acgttttgct tccggatcgt tttcgccagc cagcgctgac
     1741 aggaagcgat cttctgccgg tacgtgaaca atgttaagac caaagtgatc gccaaacata
     1801 tccagaacct gctctgcttc gttgagacgc agcaggccgt tgtcgacgaa tacgcaagtc
     1861 aggtttttac cgatagcgcg gtgcagcagc attgcggtta cggaggaatc cacaccacca
     1921 gagaggccga ggatgacttt atcgtcgcct acctgctcgc ggatgcgagc tacagcatcg
     1981 tcgataattt tcgctggcgt ccacagggct tcacactggc agatatcacg cacaaaacgc
     2041 tccagcatgc gcataccctg gcgggtgtga gtcacttccg ggtggaactg tacgccatag
     2101 aagcgttttt cttcgttagc cataatggca aacgggcagc tttcggtgct ggctacggtg
     2161 acgaagtcgg acgggatagc ggtaacttta tcgccgtggc tcatccagac atcgagcagc
     2221 ggtttaccgt ctgcggtcag cgcatcttcg ataccgcgaa ccagtgcgct gtcgtttacg
     2281 acttcaacct gcgcgtagcc aaattcacgt tcgttagagg cttcaacgtg accgcccaac
     2341 tgcattgcca tggtctgcat gccatagcaa acgccgaata ccggtacgcc tgcttcaaag
     2401 acatactgcg gcgcacgcgg gctgttttct tcagtagtac tttccgggcc gccggaaaga
     2461 ataatgccgc ttggattgaa gtcacgaatt tgtgcttctg tcacatccca tgcccacagt
     2521 tcgcagtaaa cacccagctc acgcacgcgg cgcgcaacca gttgagtgta ctgagaaccg
     2581 aagtccagaa tgaggatgcg atgcttatga atgttttccg tcattgacgc ttattccgag
     2641 gcaagtgaaa cagataatat aaatcgcccg acatgaagtc gggcgaagag aatcaggagc
     2701 ccagacggta gttcggggac tctttagtaa tggtcacgtc gtgaacgtgg ctttcctgaa
     2761 tgcccgcacc gctgatacgt acaaactccg ctttagtacg cagttcgtcg atagtaccac
     2821 agccggtcaa acccatacag gagcgcaggc cgcccatctg ctggtgaatg atctctttca
     2881 ggcgaccttt ataagctacg cgaccttcga taccttccgg caccagtttg tcggcagcgt
     2941 tatcgctctg gaaataacgg tcagaggaac ctttggacat cgcgcccagg gaacccatac
     3001 cacggtaaga tttgtaagaa cggccctggt agagttcgat ttcacccgga gattcttcag
     3061 tacccgccag catggaacct accatcaccg cgcttgcgcc agcggcgata gctttggcga
     3121 tgtcgccgga gaagcgaata ccaccatctg cgataaccgg aataccggtg ccttccaggg
     3181 cttcaactgc gtcagcaacg gcagtgatct gcggaacacc tacgccagta acgatacgag
     3241 tcgtacagat agaaccaggg ccgataccga ctttaaccgc gctgcaacca gcttctgcca
     3301 gagcgcgtgc acctgcagct gttgccacgt tgccgccgat gatttgcaga tccggatatt
     3361 tagcacgggt ttcacggata cgctgcaaaa cgccttctga gtgaccgtgg gaggagtcga
     3421 tcagcagaac gtcaacgcca gcggcaacca gcgcgtcaac acgctcttcg ttacccgcac
     3481 ctgcgccaac tgcagcacca acacgcagac ggccttgctc gtctttacag gcgttcggtt
     3541 tacgttccgc tttctggaaa tctttcacgg tgatcatgcc gatcaggtgg aattcgtcat
     3601 caaccaccag cgctttttca acgcgttttt cgtgcatttt tgccagcacc acttcacggg
     3661 cttcaccttc acgcacggtg accagacgct ctttcggcgt catgtaaacg ctaaccggct
     3721 ggttcaggtc ggtaacaaaa cgcacgtcac gaccggtgat aatccccacc agttcgtttt
     3781 cttcggtaac gaccggatag cccgcaaaac cgttacgctc ggtcagttct ttcacttcgc
     3841 gcagcgtcgt ggttggcaga acagtctgcg gatcagtcac cacaccagat tcgtgttttt
     3901 tcacacggcg aacttcttct gcctggcgtt caatggacat gtttttgtgg ataaagccga
     3961 taccgccttc ctgagccaga gcaatagcca ggcgcgcttc cgttacggta tccattgctg
     4021 cggaaagcat agggatattc agacgaatag ttttcgtcag ctgggtgctg aggtcagcag
     4081 tattcggcag aacggtagaa tgagcaggaa cgaggagaac gtcgtcaaac gtcagagctt
     4141 ctttagcgat acgtagcatg ggcaatatct cgaccagagt ggttaataaa tattgccgcg
     4201 gcattataca gagcgtaacc gattgcatct accccttttt gcaaaaaatg cttgctatcc
     4261 ccgaagggcg ggttactatc gactgaataa cctgctgatt tagaatttga tctcgctcac
     4321 atgttacctt ctcaatcccc tgcaattttt accgttagtc gcctgaatca aacggttcgt
     4381 ctgctgcttg agcatgagat gggacaggtt tggatcagcg gcgaaatctc taatttcaca
     4441 caaccggctt ccggtcactg gtactttaca ctcaaagacg acaccgccca ggtacgctgc
     4501 gcgatgttcc gcaacagcaa ccgccgggtg accttccgcc cacagcatgg gcaacaagtt
     4561 ttagttcgcg ccaatattac gctctacgag ccgcgcggcg actaccagat aatcgttgag
     4621 agtatgcagc cggccggtga agggctgctg caacagaagt acgaacagct caaagcgaag
     4681 ttgcaggctg aaggattgtt cgatctgcaa tacaaaaaac cacttccctc ccctgcgcat
     4741 tgcgttggtg tgatcacctc aaaaaccggt gctgcgctac atgatatttt gcatgtgtta
     4801 aaacgtcgcg atccttctct gccggtgatc atctacccca ccgccgttca gggcgatgac
     4861 gcgccggggc aaattgttcg cgccattgaa ctggcgaatc agtgcaatga gtgcgacgtg
     4921 ttgatcgttg ggcgcggcgg cggttcgctg gaagatttat ggagttttaa cgacgaacgc
     4981 gtagcgcggg cgatttttgc cagccgcatt ccggtcgtca gcgccgtcgg gcatgagacg
     5041 gatgtgacca ttgccgattt tgttgccgat ctgcgtgcac caacaccgtc ggctgccgcc
     5101 gaagtggtga gccgtaatca gcaagagtta ctgcgccagg tgcaatcgac ccgtcaacgg
     5161 ctggagatgg cgatggatta ttatctcgcc aaccgcacac gtcgctttac gcaaattcat
     5221 caccgattac agcaacagca tccgcagctc cggctggcac gccagcaaac catgcttgaa
     5281 cgcctgcaaa aacggatgag ctttgcgctg gaaaatcaac ttaagcgtgc cgggcaacag
     5341 cagcagcgat taacacagcg gctgaatcag caaaatccac agccgaagat tcatcgcgcg
     5401 caaacgcgca ttcagcaact ggaatatcgt ttagcagaaa ccctgcgcgc acagcttagc
     5461 gccacgcgtg aacgtttcgg taatgcagta acgcacctcg aagccgtaag cccactgtca
     5521 acgctggcgc gtggatacag cgttactact gctactgacg gcaaggtact gaaaaaagtg
     5581 aagcaggtta aagcgggtga aatgctaacc acacgtctgg aagacggctg ggtagaaagt
     5641 gaagtaaaaa acatccagcc ggtaaaaaaa tcgcgtaaaa aagtgcatta agccagcacg
     5701 aattcaacgc gttttttcga tatcaagcca tgcccgtgct gacagaagta atctaccgca
     5761 ccgcaggctt ttaacacctg caacggttga tggcagtccg ggcaaagagc tttcatttca
     5821 ataactttcc cgcaggatgg gcaatacgca tggccgttat cctgatcaag cacatgctga
     5881 cattgtggac aatgcagttc catatgaatt cctccggtag ccacccctct acattcatag
     5941 agggaatggc agataaaata cttacggata attatttatt tttcttgatg tgcttcatca
     6001 gacgcttacg tttacgcatc tgggttggcg tcagggtgtt acgcttgttc gcatacgggt
     6061 tttccccttc tttgaactga atacgaatcg gcgatcccat tacgtccagc gatttgcgga
     6121 agtagttcat caagtaacgc ttgtaggaat caggcaggtc tttcacctga ttaccgtgaa
     6181 tcaccacaat cggcggatta taaccaccgg cgtgtgcata tttcagcttc acacgacgac
     6241 cgcgtaccag cggcggttgg tgatcttcaa cagccatcgt catgatgcgc gtcagcatag
     6301 aggtccccac acgacgggtg gagctgtcat acgcttcacg tactgattca aacaagttac
     6361 caacaccact gccgtgcaag gcagagataa agtgcacacg agcaaaatcg ataaagccca
     6421 gacggaagtc cagcgtttct ttcacctgct ctttcacttc ctgactcagg ccatcccact
     6481 tattcaccac aatgacaagt gagcgcccac tattgagaat aaagcccagc agcgagagat
     6541 cctgatcgga aataccttcg cgcgcatcaa tcactaacat caccacgttg gcgtcttcaa
     6601 tggcctgcaa cgttttgatt acggagaatt tctctacagc atcggtgatt ttgccgcgtt
     6661 tacgtacgcc agcggtgtca atgagcacat actcacgtcc atcgcgttcc attggaatgt
     6721 agatactgtc acgcgtcgta ccaggcatgt cataaacaac aacgcgctct tcaccaagaa
     6781 tacggttagt gagtgtagac ttacctacgt tcggacgacc cacaatcgcc agtttgatcg
     6841 gcagactttg cgggtcgaag tcgtcttcct cttcttcttc gccgttctct tccgcttcaa
     6901 attgcgccca gtattcagcg tcttcgtcga cttcctcttg cggtgcgaga tcttccatcc
     6961 acggcagtag cacatgctcc agcagactta atacgccacg accgtgagac gcggcgatcg
     7021 ggtagatttc acctaaacca agcgagtaga aatcaaccac tgcctgatcg ggatccagac
     7081 cgtcagtttt gtttgccacc aggaaggtcg gtttttcacg ggagcgcaga tgtttggcaa
     7141 tcgcttcatc tgccggcatc aggcccgcgc gcgcatccac cataaacagt acgacgtccg
     7201 cttcttcaat cgccagcagc gactgttccg ccatgcgggt ttctacaccg tcttctgtgc
     7261 catcaatccc gccggtatca atacagataa actcacggcc ttcaatttcc gcacgaccgt
     7321 acttacggtc acgagtcaga cccgggaaat ccgcaaccag cgcatctcgg gtgcgagtta
     7381 gacggttaaa taacgtggat tttcctacgt tagggcgccc gacaagcgcg accacaggta
     7441 ccatgtttaa agcctcattt ttataaatca tcagacaacg cacgctatat tcgcgtcgtt
     7501 gttaaaaaca ggaaaacggc ccctgtccag gagccgtttt caaagtgaac gacagagacg
     7561 attaacgtgt aatagagtac acggttccgt cttttgcctg gatcagcagt ttgccgtcag
     7621 cggcaaccgg ttcagtctgg aaaccggaac tatcaacttt ttgctgggca acgaaacgac
     7681 catcttcgac gttaatccag tgcaaataac cttcactgtc accgaccacc aggttgccat
     7741 tatacagcac cggagaagtc agcaggcgat gcagcagatc gctttgtgtc cacagcgtaa
     7801 cgccgccatc aatggtcaac gccatcaccc ggtcattttg atcgaccaga tagatgcgat
     7861 tgccgtcgac gatgaaatca ttcaccgaac ccagttcgcg tttccacata atctgaccac
     7921 tgcgcagatc aagcgccgtc aggttaccat tataggccag cgcgaaaaca acgccgttaa
     7981 caacggcggg agtcgtgtca acatcgctca gacggtcaat ttcggtagaa ccggtcgctt
     8041 gggaaatacg ctgctgccaa atcatctggc cctgttccat cagcactgcg ctgacgcgac
     8101 cattatcgcc ccccacgacg gccgcaccaa aagccgttgc cggcgcagac tcgccacgca
     8161 aagagagcga aggcatatcg aggttaactg tccatttgac agcgccgtca gcttcgttca
     8221 gcgcttgtaa ctgaccgtta ctggtgtgga ttaacaccag accgtcgctg accaccgggc
     8281 gcgaaagtgc ttcacccgcg actttagttt gccatgccac agtaccatcg ctggtattca
     8341 gcgcgtaaac ctgcgccttt tcgctgccaa tgtagacatg tccaccagac acggtcacac
     8401 cgccagaaag taatgcaggc tctttagaga accagccatc tttctcggcc aggctgacag
     8461 accagatttc tttgccatca tccgcattca gcgcttttac taaaccagcg cggtccgctg
     8521 catagacaac gttgtccgcc agtgccggat gaagattgga atagaagttg ccaatgccgc
     8581 taccaacgga agtgctccac gccgtggtcg gcgtaaactg gttttcaacg gttggcaatg
     8641 gggacatctt taccacatct tcttcgctgt taaacagcga acagccgctt aaaagggtaa
     8701 cggaaagcag tcctggcagc agtaatttac gcaattgcat cgggtccctc tcagatggac
     8761 aaattattaa ttttcatctg catcatttcg ctcagtgccg gagtaacatc gcttttcacg
     8821 cctgcttccc atgcactacg cgcaccttgc ttatcacctt tgctcagcaa tgcttcacca
     8881 cgcaggtcgg caacaatggc agcccaccct tcacctttga tggtatcaag ggttttcagc
     8941 gcggcatcag cctgcttgag ctgtacctga acgcgagcaa gacgcagatt tatcacggct
     9001 ttgagatttt catcgctcgt gtctgccagc ccctgttgta actgggcggc agctttctct
     9061 agttcatttt tgtcaacaaa ttgctgcgca agttccaaag aagccagcgc accataagta
     9121 tttttatttt cagcagcaaa tttttccgcc gccgggatgc tatccggttt gccttcgctc
     9181 actgcggtaa ccgcattttg ataggcaaga gaagcggagc gtgcagaatc aacctgatgg
     9241 ctgttccagt agcgccagcc aatcagtgcg ccaacgccca aaatcacccc aacagccagt
     9301 gctttgccat tttcagcaaa aaagcgttta atcgcttcta cctggtcgtt ttcgttctcg
     9361 taaatttcca cgctgtcctt ctccttcctt aacccagtaa cgtgcgcaaa tgcgcggcta
     9421 cgctatcctg cgcaactgcc gtttgctcac cagagcgcaa atccttcact actgctgtgc
     9481 cgttagccac ttcagactca cccagcacca cagcaacgcg ggcaccccat ttatcagcac
     9541 gggcaaactg tttcttaaag ttgccgccgc cgtggttggt catcaatttc acgcccggta
     9601 attcatcacg cagacgctca gctaatgcca tagccgcaga ttgtgtatca gcacctgaag
     9661 ccaccaggta tatatcgaca acaggatcgg ctttaaattc cggattaacg gcctgtacta
     9721 acaatacaag acgttcgagg cccatcgcaa aaccgacagc cggtgttgca cggccgccca
     9781 gttgttccac aagaccgtca taacgaccgc ctgcacacac ggtgccctgg gagccgagac
     9841 tgttagtcac ccactcgaaa acggtacggt tgtagtaatc cagaccacgc accagacgct
     9901 ggtttacggt gtaagcgatc cccgcgctct caagcagttt gcacagaccg gcaaaatgct
     9961 cacgagattc ctcgtccaga taatcaccta atgccggagc gtcgttgaga agcgcctgca
    10021 cttccggatt ttttgaatcc agcacgcgca gcgggttagt gtacatgcgg cgtttgcagt
    10081 cttcgtccag cttttcttta tgctgctcaa ggaatgccac cagcgcatcg cggtaattgg
    10141 cgcgtgcttc cagcgaaccg atagaattca gctcaagagt tacgtgttcg gaaataccca
    10201 gcgcgcgcca ccagcgggcg gtgagcataa tcagttcagc gtcgatatcc ggaccttgca
    10261 gaccgaaaac ttcgcagccc aactgatgga actgacgata acgccctttc tgcggacgct
    10321 cgtgacggaa catcggcccg atataccaca gacgctgttc ctgattgtac agaagaccat
    10381 gctcgatgcc ggcgcgtaca cagcccgccg tcccttcagg gcgcagagtc aggctgtcgc
    10441 cattgcgatc ctcaaaggtg tacatctctt tttcaaccac gtcggtgact tcaccaatcg
    10501 cacgtttgaa tagcggggtc tgctctacaa tcggcaagcg gatttcactg taaccgtagc
    10561 tgccgagcac gtttttcagt gtgccttcaa tgcgctgcca gatggccgtt tcgccaggca
    10621 ggtaatcgtt catgccgcga atggcttgaa tgttttttgc cacgtttatt ctctttctga
    10681 atataaaaat gaaccctcaa cgcttccctc aatgtttcgg gagccatgcg ggttcaatca
    10741 tacacgggaa gcgaggcgct tcccatcacg ttattatttt tcaacctgct gaacgtcaat
    10801 tcgacgcgct tcgtccagct gactggcttt cgcacgaatg cgtgcctcca gctggtcgat
    10861 catatcgttg ttgtccagac ggtctttgcg cacgccatct tcatagagac cgcttttctt
    10921 gttgccgccg gtgacgccga gtgtagaaac cagcgcctca cccgggccat tcaccacgca
    10981 gccgataatc gaaacgtcca tcggagtgat gatatcttcc aggcgttgct ccagcgcgtt
    11041 aactgtaccg ataacatcaa attcctgacg cgaacaggtc ggacaggcga tgaagttgat
    11101 acctcgcgaa cggatacgaa gcgatttcaa aatatcgaaa ccgactttga tctcttcgac
    11161 cggatcggcc gccagtgata cgcgcagcgt gtcgccgatg ccttcagaca gcagcagacc
    11221 taaaccaatg gcggatttta ctgccccgct gcgcgcgcca cccgcttcgg tgatccccag
    11281 atgcagcggc tgatcgattt gttttgccag caaacgataa gactcaacag cgaggaagac
    11341 gtcagacgct ttcacgctga ctttgaactg atcgaagttc aggcgatcga gatgatcaac
    11401 atggcgcatg gcggattcca gcaacgcctg cggcgtcggt tcgccatact tttcttgcag
    11461 atctttttcc agcgatccgg cgttaacgcc aatacggatc ggaatgtttt tatcgcgcgc
    11521 acagtcaacc accatgcgaa tacgctcttc attaccgata ttgccagggt taatacgcag
    11581 acaatcgacg ccgtattccg ctactttcag cgcaatgcga tagtcgaagt ggatgtcagc
    11641 caccagcggc acgttaacct gctgtttgat gagtttgaac gcttctgccg cgtccatcgt
    11701 cggtacggag acacggacga tatcagcgcc aacgcgttcc agcgccttga tttgattgac
    11761 cgttgcttcg acatctgtcg tacgcgtatt ggtcatggac tgtacggcga tgggagcacc
    11821 atcgccaatc ggcacattcc caacgtaaat acgtgttgat tttctacgtt gaattggagc
    11881 ctggttatgc atgaaaaatc tcccgcgtta cccgtctgtt actgcgccgg tgattgttcg
    11941 gcattgaggg tcagacgcgc aacctggtta gttctgataa aacgactcag atcgacaggt
    12001 ttcccttgat actggatctg tactgcggct ggcgcaccaa ttttcagttt gtacggtgcc
    12061 tggccggtta agttcaaatt accgtcttta cgctgcatac cgctaaacaa ttttttaccg
    12121 gtagcatcag tgacctccag ccagcaatcg gcagtaaagt tcatcaccag cgcattcgga
    12181 tcagccaccg gcgtggtcac gccagcctga tcggttggca acggcgcagc accatctggc
    12241 gttgttgctg ccgttggtgc cggggtcgcg gcggtatcaa catttgcctg cgaaggcgaa
    12301 acaaccgcat tctgttgcgg atcaacagct ggtgctggcg cagttacgac aggtgtttgc
    12361 gtgttggttg cggtagtatc cacagacgct ggcggcgtgg acgttgtagc cgggtctgta
    12421 gttgtcgacg tatttaacgg aacactctgc ccctgctcgc tattactgct cagttccgcc
    12481 gaagattgat cggccatagt ggtgatctct tcctgctgag ctttgtggtc ttgccaccac
    12541 caggcaccgc tcaggccgat aaccacaaac aacaccagcc aggtgaaggt catcagccag
    12601 ccgtcgcgtt ttttgcggcg tttaccgagg gaaaaactct gcatcggcgc aacttttgca
    12661 gcccgtagtg gagcctgctt ttccagccct ggcagcagtt cttcttctgg aatatgtacc
    12721 agacgcgcat aagagcggat atatccgcgc aggaatgttg aagcaagatc ggcgggtgcc
    12781 ttatcttctt caatgtcgcg taccgtggaa accttcaggc aaagtcgctc ggcaacggcc
    12841 tgctgactaa gtcctagttg ttcgcgagca ttacgcaggc gagcgccggt agtaagtgct
    12901 tcattttggt cgtgcgtggc ttcagtattc attcgctgca ggtacgttta aatgagaatt
    12961 gagatgccgg tgaaccatca tgctcaccca caccgcgaaa catccgttaa gttaaccgtt
    13021 atcatacagt ataagaccgt ctgtgtgctc gtgacaaagc ccacattcac taaggctaac
    13081 ttactgttgc atcgttacat actgccttaa agtcagcaaa aacgcaccgt taatattgac
    13141 cagacaattg caacttaata cttcattcat aaaacattac gccacggtac ataaagtaac
    13201 cgtggcgtaa tggctatcag accgctttaa tgtcgatggc ttcaccctgc atccgtttac
    13261 gcagggtacg tttcgtacgg tcgataacat cgcccgccaa ctgaccacag gcagcatcga
    13321 tatcatcacc acgcgtttta cgcacaatag tggtgaaacc gtagctcatc agcacttttg
    13381 agaaacggtc gatacggctg ttcgagctgc gtccatacgg cgcagccggg aacgggttcc
    13441 acgggatcag gttgatctta cacggcgtat ctttcagcag ttccgccagt tggtgcgcgt
    13501 gttcagtgcc gtcgttaacg tggtcaagca tcacgtattc aatagtgact cggccctgat
    13561 tggcgttgga tttctccaga taacggcgca ccgcagcaag gaacgtttcg atattgtact
    13621 ttttgttgat cggcacaatt tcgtcacgaa tttcgtcgtt cggcgcgtgc agggaaattg
    13681 ccagcgcaac atcgatcata tcgcccagtt tatccagcgc tggaactaca ccggaagtgg
    13741 aaagcgtcac gcgacgttta gacagaccaa aaccgaaatc atcaagcatg atttccatcg
    13801 ccggaacgac gttgttcagg ttgagcagcg gctcgcccat gcccatcatc actacgttag
    13861 tgattgggcg ctgaccggtg acttttgctg cgccgacgat tttcgccgca cgccacacct
    13921 ggccaataat ttccgatacc cgcaggttgc ggttaaagcc ctgctgggcg gtggaacaga
    13981 atttacactc cagcgcacac cccacctgcg aagagacgca gagcgtagcg cggtcgtctt
    14041 ccgggatgta caccgtttcg acacgctggt cacccacggc aatcgcccat ttaatggtgc
    14101 cgtcagatga acgctgttct tcaaccactt ccggtgcgcg gatctccgcc acctctttca
    14161 gtttgccgcg caacactttg ttgatgtcgg tcatctcatc aaaattgtcg cagcaatagt
    14221 gatacatcca cttcatcacc tgatcggcgc ggaagggttt ttcacctaaa tctttaaaaa
    14281 actcccgcat ctgctgacga ttgagatcca gcaggttgat ttttccatct ttggtggtga
    14341 cgttttcagg tgtgactaat tgttcagaca tatgctattc cggcctcgtt attacacgtt
    14401 atggcccctg gagggttgaa aaaagaaacg ccccggtgag cggctgctcg tccgggggcg
    14461 ctgcattgta caaattctgg cgcacggatg ccacgtttgc acgcggcatt tacgaaatta
    14521 ttaacgggtg cgcgggcaca cttcgccttc gccgaagaaa taagcgattt cgcgagcggc
    14581 agattcgacg gaatcagaac cgtgggtacc gttttcggtc aggctgtcag cgtaatcagc
    14641 gcgcagagta ccggccagtg cgtttgccgg attggtcgcg cccagcagat cgcggtgacg
    14701 ctgaacggcg ttttcacctt ccagcacgga aaccacgatc gggccagagg tcatgaattc
    14761 aaccagacca tcaaagaacg gttttccatc gtgttcagca taaaagccac gtgcctgttc
    14821 aacggtcagg tgcagcattt tggtgccgac aattttgaac cctgcagctt caaagcgcgc
    14881 aaagatatta ccaatgacgt tttttgctac cgcgttcggt ttgataatgg aaaaagtacg
    14941 ttcaatagcc atttttacct ctgtaaattg ttctgttgtt gtctgtacca gcgtacgaga
    15001 tggcgcggat tataatgagc aacagagccg ttgactattg atgaaggtaa cattttttta
    15061 aaataaaatg agttttagca acaactcgac ttttatggtg ttctcatccc ataacgatga
    15121 cgttggcaat aaagacaatt ttgcgcattt tgctgaaaag taagaaagga tttttgacag
    15181 gttttacaga ctttgcgatt agcatgaaga ttcacaattt ttgccggtag aatcgtcaat
    15241 tcaacggtca ttgcctgatg aaaacaaaca gcaatacaat tttggcatcc gttacacaag
    15301 gcatcattta tttgtaaatg gccttcgtta agcgaaaaga cctgttgctc gcaggcccgc
    15361 cagcatgcac cgcaaagctg acacttttga ggatccatca tcgggcgagc atcactaatc
    15421 tggggccata aataatgtaa ccgacgtaat cctggttgta tttgtgtcat ctgaacatcc
    15481 gcacgctgca catggaacaa acgacgccgg gaaacattca cctcactggt atgtggagat
    15541 accacagacc agccttcctc gccatattgc cgtaagcatt cgtttaacag caataatgcg
    15601 cgctcccatg ccttcgcctg actcagatca atcgcgatcg ctgtgatccc ctgctcttta
    15661 tgccatagca aaagttcttc cagcgtcggc gcaatagtgg tgaaaggtgc gaccagggtg
    15721 tgattgcaaa aataacgtaa aggggaatta acgccacgaa tagcctcttg cggacaaata
    15781 aactggcaaa ccgcacaatc aatacattgt tccgcgtcga ccgctatgcc ctcttctgtc
    15841 agcgttagcg cctgtgccgg gcaagcctcg acacaggcat gacatgttgc aaaacgaaag
    15901 cgttggcgcg cgcattccgc gccaatcgtt atgttatgac ttaacattag ccgatactaa
    15961 aaaagacaaa acgcagcata acttcactcc ccactagcat tacgctaccg aataacgccg
    16021 ccgggatatt ctttttacaa ccaccgacag caaagaacat catcccaatg acggaaattc
    16081 cccagcttag tgtcagtatg ggttttaact gtcccagcat cgctaatggc gcatgtggca
    16141 atgtggctat ctgcatcgca tttgccgtta aatcccctat ccacaacggc tgaaccaaca
    16201 aacgcagcag aaccaggaga gcgatcacca acacgcatcc attacgcagg gcatcaatct
    16261 gcggcaaaat tccgctaata cgaaacactg ctaccagcgc tgaaccgaga attcccactg
    16321 tacctaagaa cataatcagc gtgttgtaat gctgccaggt gataatcgac gtatggatgt
    16381 aaacttgcgc catgcagaaa acatcaacaa tcccgaccac tgccgctagc ataagcagga
    16441 gttgccagcc gcctttgcgt aacatgacca gtaatgtgga taaacccaat agcgccagat
    16501 acagcgcacc aaagataatt tcccgactca gccatgaact ggaaaagtgg ctcagcgcat
    16561 gcatcgcatt aagtggataa cccaggtgta aggtagaaat cagtaaccct acggcacctg
    16621 ccaaaaaagc caacagtaat ggcggccgca taaccgggcg ggcgctggag gtgattaaaa
    16681 tatgacgatg aagaaacgcc agccacaacg tcataccgac cgatgcctgt aacaacagcg
    16741 taaaagccat taacggtaat tcatgcatga tgtgacgcct ccttttccgc gccctgatgc
    16801 ggtttaatca ccagatttgg ctgcgtaatc gacgaatcag gtaaaccttt cacatcactg
    16861 acatcgccat acttttcacg caactcatca atagggccga acttaatcgc ccccaacggg
    16921 cacgttgcca cgcaaatggg ttgttctcct ttagcttgca gatcaataca gaaatcgcat
    16981 ttcgacatct gcccggtctg ctcattcatt tgtggtgcgc cgtaaggaca ggaccaggcg
    17041 cagtaaccgc aaccgacgca cttatcagta ttcacgcgca caataccgtc tccgggacgc
    17101 ttatgcatcg ccatcgtcgg gcaatttttg gtacagatcg gatccgcgca atggttacag
    17161 gaaacagaaa gggtataagc aaagacatta tgactcacac cgccctctcc agtggcgatg
    17221 aagccacctc cgttcacttc atacacgcgg cgaaaacgtc gcccaacctc aaggttgttt
    17281 ttgtctttac aggccacctg acacgcttta cagcccgaac aacgcgaaga gtcaataaag
    17341 aaccctaatt gtttgtcgct gacagatgca aattctttaa attggctcat gctttctcaa
    17401 cctccaccag cattgtttga tgcgaattcc ctttcgccag cgccgtaatg cgggtcgaac
    17461 tcagtacatt ggcacagcct ccgcgatcga taccttgtgc atccggttgc caccatgccc
    17521 ccgcctgcat agcaactacg ccagggatga tacggggggt tatttccacc gggataatgc
    17581 tcacaccacg atcgttataa atttttacgc tatctccttc gctaatcccg cgctgttttg
    17641 catcttgcgg attcagccag agtttttgcg tctgaacttc ttgcagccac gggttagcat
    17701 actgggtgga attagcacgg tttttccctt tccaggtgat gagctgcaac gggtatttcg
    17761 ccgtcagttt atcttctggc ccttcaaagg cagggacata gtgcgaaagc gcgggaattt
    17821 ctggatcttg catatcaaaa agacgcttag agaagatctc aattttgccg gaaggcgtcg
    17881 ggaaaggatt attctgcgga tcgcgaatat ttgcttcgaa agcgatgtgc ggcgcgcttt
    17941 tgaaaagatg tcgacgtgtt ttttgcaggg ttgcgaagtc cggcaaattt tcatccggca
    18001 tcgccagacg agtttgctcc cagatatgct cgatccattg cttctcatca cgtccttgac
    18061 tgaactcggc ttcgacaccc agttttttcg ccacgtcacg caaccagtca taatcggtgc
    18121 gacgttcaaa atccggctca atcggttttt ctgacagaat aagatagctc gcagttcccc
    18181 atgtttcgcc gatattccag cgttccataa agctggtttc cggcagcaac acatcggcat
    18241 atttcgcgct cggagtcatg aacagatcgc tgagtacgat gaattctatt ttagattcgt
    18301 cttcaaggag cttcgcggcc tggtggacgt ccgggttctg gttcgccaga taattacctg
    18361 ccagtgagaa cagcaggcgg atattactgt cgagtttatc gacaccttta agcccatctt
    18421 gcggcgtaac tttagaagca tcatccgccg cctgcatcca gttcataatc gagattttcg
    18481 cttgtaccgg attttctggc atatctggtc caacgcagaa tttacgatta gaactaccgc
    18541 cgtaacctgc cgcccagcca cctttgatgc cgacgttacc ggtaatcgag gccagtaaag
    18601 ttgaacctcg ggcggtgcgt tcgccgcaaa tatgtcgctg tggcccccat ccctgaatca
    18661 acgctgcggg ttttgtagtg gcgtaatcgc gtgccagttg acgaatggat tgtgctggaa
    18721 cgtgggtaat tttttctgcc cattccggcg ttttgtgtat cccatctttt gcgccaaaaa
    18781 ggtaggcaac caacgattcg ttggctggta cgccttccgg catggagttt tcatcaaaac
    18841 cgagagtata tgtgtcgata aatgtcttat cgtggagatt ctcgctgata atgacgtaca
    18901 tcatcgcgtc catcaacgca ttgtccgtgg tcggcaacag cgggatccac tgatcggcaa
    18961 gtgaagatac ggtgtcggaa tagcgcggat ccaccacgat aaaacgtgta ccgttttgct
    19021 tcattttttg aaaatagtaa ttggtgtgac cgaatatggt ttccgttggg ttatgccccc
    19081 agagaatcac cagtttggta tccagaagag tatccatcga gttaccgctc gcggccacgc
    19141 catacgtgta aggcgtggcc gccgcagtat tacccagact aactgagtga taatattcaa
    19201 gataaccacc ggttaaattt aacagccgac gcgccatcgc atcgccagaa aatgttccac
    19261 cagaaacagc cgtcccgaca tggacataac gtgaagctgg gccatattgt tgcgtgatcc
    19321 ttttcaggtt atcggcaatt aaggtcgtcg cctcatccca tgagatacgc tcaaatttac
    19381 cctcgcctcg tttacctacc cgcttcatcg gatatttcaa acggtctgga tgataaacaa
    19441 atttacgata gccgcgacca cgaacacagg cgcgcataat cggcatttgt ggatccaatt
    19501 cgttatcggg aagcgtggta atttgcgtca ccacgccgtc gcgcatatgc gcccgaatat
    19561 cgcacttacc accgcagtca aatgtgctac aggtgggcac gactttatca gctactggag
    19621 ctgtaacttc tggcgacgca tctggggatt gcgcctttgc ggtagaagaa ataaaaggca
    19681 aagtgataag cgcggaagtc gcttgtaaaa aactacgtcg tgatacttgc ggaaatgtaa
    19741 tgcgttcctt ttcgtctggc gtatccatct tgcgttcccg aaaatgaaaa aataaatatt
    19801 cacgtattta ttcgagaaca atgct
//
LOCUS       DBFIFM010000074        19818 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.074, whole genome shotgun sequence.
ACCESSION   DBFIFM010000074 DBFIFM010000000
VERSION     DBFIFM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.074"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            206..706
                     /gene="tssB"
                     /locus_tag="N7C74_002304"
     CDS             206..706
                     /gene="tssB"
                     /locus_tag="N7C74_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HGU2432165.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSSLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDEMNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSNK"
     gene            741..965
                     /locus_tag="N7C74_002305"
     CDS             741..965
                     /locus_tag="N7C74_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432166.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1016..2407
                     /gene="tssC"
                     /locus_tag="N7C74_002306"
     CDS             1016..2407
                     /gene="tssC"
                     /locus_tag="N7C74_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HGU2432167.1"
                     /translation="MSLQEEELVSSHAGQPEQESSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWY"
     gene            2498..2911
                     /gene="tssE"
                     /locus_tag="N7C74_002307"
     CDS             2498..2911
                     /gene="tssE"
                     /locus_tag="N7C74_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HGU2432168.1"
                     /translation="MILDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2915..4765
                     /gene="tssF"
                     /locus_tag="N7C74_002308"
     CDS             2915..4765
                     /gene="tssF"
                     /locus_tag="N7C74_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HGU2432169.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4729..5811
                     /gene="tssG"
                     /locus_tag="N7C74_002309"
     CDS             4729..5811
                     /gene="tssG"
                     /locus_tag="N7C74_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HGU2432170.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTTFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5836..7116
                     /gene="tagH"
                     /locus_tag="N7C74_002310"
     CDS             5836..7116
                     /gene="tagH"
                     /locus_tag="N7C74_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HGU2432171.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHAPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDHNDDISAAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7113..7637
                     /gene="tssJ"
                     /locus_tag="N7C74_002311"
     CDS             7113..7637
                     /gene="tssJ"
                     /locus_tag="N7C74_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HGU2432172.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLQAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPIKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7640..8971
                     /gene="tssK"
                     /locus_tag="N7C74_002312"
     CDS             7640..8971
                     /gene="tssK"
                     /locus_tag="N7C74_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HGU2432173.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAELLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADVH
                     DSELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8976..9737
                     /gene="icmH"
                     /locus_tag="N7C74_002313"
     CDS             8976..9737
                     /gene="icmH"
                     /locus_tag="N7C74_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HGU2432174.1"
                     /translation="MDEGSLSLPPFTGYDEKSLRDYHLALHGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAVQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9746..12517
                     /gene="tssH"
                     /locus_tag="N7C74_002314"
                     /gene_synonym="clpV"
     CDS             9746..12517
                     /gene="tssH"
                     /locus_tag="N7C74_002314"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HGU2432175.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDASDTLLARYAKNMTEDARNGRLAPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYERSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESEQDNTGAVPADETDREQPEETAETVSPVQRLAQLTAE
                     LDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTIKGQD
                     LAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTI
                     NMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLF
                     YQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEALYPVLADFFK
                     PALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIMSRVT
                     RAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDAQNDE
                     SVTKDETVL"
     gene            12514..13257
                     /gene="vasI"
                     /locus_tag="N7C74_002315"
     CDS             12514..13257
                     /gene="vasI"
                     /locus_tag="N7C74_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HGU2432176.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDHLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13262..14674
                     /gene="tssA"
                     /locus_tag="N7C74_002316"
     CDS             13262..14674
                     /gene="tssA"
                     /locus_tag="N7C74_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HGU2432177.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPDWEYIDGEIVK
                     LGSLAHAQLDIPELQRRGLQLLASESKDFRLLAHLLRTLQHAGDPLLALHLLTLYVEH
                     YWTVAAPQNMAHKKRFASQIIKRFETGIEGFSQNAATTQRDTLLGELAKLAQCWQSHN
                     VPELAQATDDLFALYQRTFNRAAPAPVPTPAASGSSPQTTVTSESGVTQPSAPAPQIA
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQSITSTPQAESDGRTPLAA
                     VSADMVADYQSRLASADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADTIR
                     DEVIRFLERLPQLTGLLFNDRTPFLSEQTKQWLAASPDGKVAPVAQIGEESQAARACF
                     AGQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLIQLAQQQYRMLLMIGSQMM
                     VSDWEPSLLTQLEQKFTAEQ"
     gene            14811..18245
                     /gene="tssM"
                     /locus_tag="N7C74_002317"
     CDS             14811..18245
                     /gene="tssM"
                     /locus_tag="N7C74_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HGU2432178.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQLLEKQQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGTEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRHREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVTQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLMRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENITLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLTDQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18256..19665
                     /locus_tag="N7C74_002318"
     CDS             18256..19665
                     /locus_tag="N7C74_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HGU2432179.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQLKSNTENAAQTTVRTLIYAPETQASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYQDARAQKQNTDALEKQINERLEGVLSRWLLLKNNTIPTIKKALNFNN
                     IHEYKGVLNGEFNLFNTKW"
     gene            19631..>19818
                     /gene="tssD"
                     /locus_tag="N7C74_002319"
     CDS             19631..>19818
                     /gene="tssD"
                     /locus_tag="N7C74_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HGU2432180.1"
                     /translation="MANLIYLTLNGDNQGLISSGCSSQPSIGNKAQTAHIDQIMIYEF
                     MHGLNRDQNVNHHHLTIK"
BASE COUNT         4646 a         5464 c         5490 g         4218 t
ORIGIN      
        1 taaaatctat ttaacgcttg cagaataccc ctctttacga tcgataaaaa gtccttttct
       61 tatttatttt gaagcgtaaa atgcggggtg atgaaggagt ggtgatttta ttttcaccgc
      121 catcaagacg gcatttccga tattttttgc ggtacgaata tgccgtgttc atggcctgta
      181 cgagataaca tgacggatca cttttatgag caaaaaattt gaaggcagcg tcgcgcctcg
      241 cgaacgtatt aatatatcgt atgtcccaaa aacagatggc cagacggctg aagtggaact
      301 tccgcttaac atgctcgttg tcggcgatac cggaaatacg caggaaacat cctcactgga
      361 tgaacgtcag gcagtttccg ttaacaagca caatttcggt gcagtgatgg ctgaagcagc
      421 cattggcctc aattttaccg tccccgccac gctgaaggga agcacaacgg acgatgaaat
      481 gaatgtggcg ctgaatatca agtcactgga tgatttttca ccagatagcg tggcccggca
      541 ggttccggaa gtgaataaat tgctggaact gcgtgaagca ctgactgcac tgaaggggcc
      601 gatgggtaac cttccggcat tccgtaccca gctccaggca ttgctggaaa atgaagagtc
      661 ccgcgagcag ttgctcaaag aaatcggcca ggtgagcaat aaataaccac cactatttca
      721 ttaaacaggg ttatccaggc atgacggcac acaaaaatat ctccgggaca taccacctct
      781 ctgttgcaga cattctgcag gtggtttacc aggtatgttt cagcccgtcc gttgaaatca
      841 accaggatgg tgtggctgcg ctgattacaa cactggatcg ccgtatcagt gatctattgg
      901 atgagattat ccatttttgt gagtttcagc agagcgcgtc gcactggcag cgcgtacttc
      961 actgacggcg aaaagccggt ttcagaaaca cagctaaaac agaaggattt tcagtatgtc
     1021 tttacaggaa gaggaactcg tttccagtca tgccgggcag cctgagcagg aatcgtcgct
     1081 gcttgatcaa atcatggcac agacccgtat tcagcccggt tcggaaggtt atgacgttgc
     1141 ccgccagggt gtgacagcct ttattgccag catcctgcaa tccactgcat ctgccgaacc
     1201 ggtcaataaa ctggccgtgg acagcatgat tgcagatatt gatgaacgca tcagccgcca
     1261 gatggatgtc atcatccatg cgcctgcctt tcagcaggtc gagtcgttct ggcgctcact
     1321 gaaaaccatg gtggatcgcg ttgatttccg tgaaaacatc aaggtcaacg tgcttcatgt
     1381 caccaagcag gaacttctgg aagactttga gtttgcgcca gaaattattc agtccggatt
     1441 ttataagcac gtttattcgt ccggtttcgg acagtttggc ggtgaaccca tcgctgccgt
     1501 gctgggcgct tatgagttca aaaataccgc gcctgacatg aaactgctgc aatacgtcag
     1561 tgccgtgggc gcaatggcgc acgcaccgtt cctgtcctcc gtttccccgg agtttatggg
     1621 gctgaactcg tggaccgaac tgcctaatat caaagatctt tatgccatct ttgaaggtcc
     1681 ggcctacacc aaatggcgcg ccctgcgtga ctcggaagat tcgcgctatt tagggcttac
     1741 ggcaccccgc ttcctgcttc gccagcccta ttcaccaaca gataaccctg ttaagaactt
     1801 taactattac gaagatgtca gccagaacca cgaagattat ctgtggggta atacggcctg
     1861 gatgctggca tgcaatatcg cagacagttt tgccaaatac cgctggtgtc caaacattat
     1921 cggtccgcaa agcggcggcg cagtgaaaga tctgccggtg catctgttcg aaacgatggg
     1981 gcagattcag gccaaaatcc caaccgaggt gctggtcacc gatcgccgcg aatttgaact
     2041 ggctgaagag ggttttatca cccttaccat gcgtaaagac tctgataatg cagccttttt
     2101 ctctgcaaac tcggtacaaa aaccgaagca ctttccggga aaagatgcag aaaccaacta
     2161 taagctgggt acgcagcttc cttatctctt catcatcaat cggttagcgc actacatcaa
     2221 ggtgttgcag cgtgaacaac tggggtcatg gaaagaaagg agcgacttag aaagagagct
     2281 taatacctgg atccgtcagt atgttgccga ccaggaaaac ccgcctgcag acgtgcgcag
     2341 ccgcaaacct ctgcgcgctg ccaaagttga ggttatggat gtagaaggcg aacccggctg
     2401 gtactaggtt gcgctaagcg tgagacctca ttttaaattt atgggggcaa attttgagct
     2461 ttccctggtt ggccggttag acagggagta acacaccatg atactggatc gggaaagaac
     2521 cacaggttct ctttttgagc gtatggaagc ctcttcggcc agaaaccggc agggaggcag
     2581 tattcattcg ctcaggcaat ccattcgcca gaacctaaga aacattctta atacccggtc
     2641 cggtagttgc cggggtgcgc ctgagctggg cattgatgaa ccggaagggg ctgaaaactt
     2701 ccgcgaatcc atgagtcggg ccatcgagca atgtatagaa cgctatgaac cccggatctc
     2761 acacgctgaa gtgcaggcag tggtttcatc cgcctccagc ccgctggata tgacgtttca
     2821 tattacagcc tgggtgacat ttaacgaaac gcatgaggtg cttgaatttg atatggcacc
     2881 gaatggcagc cagcattacc gggtggactg acaaatggaa tttgaagaac gctattttcg
     2941 ggaagaactc gattacctgc gccagcttag caagctgctg gcaacggaaa aaccccatct
     3001 ggcccgcttc ctggccgaaa aagatgcgga tccggatatt gaacgcctgc tggaaggggt
     3061 ggcttttctt accggcaatc tccgccagaa aattgaggat gaattcccag aactgacgca
     3121 cgggcttatt aagatgctat ggcctaatta cctgcgtccg gttccggcaa tgacccttat
     3181 tgaatatacg ccggatatgg ataagtcttc tgtaccggtg ttaatccccc gtaatgagca
     3241 gtttacaacc aacgccgggg aaatcagagt tgatgaagtg ctgccctctg atgctaaaaa
     3301 ggaggagccg cctccctgta ccttcaccct ctgccgggat atctggctgc tgcccgttcg
     3361 cctggagcag attgaaaacc gcagtacgac ccgtaatggt gttatcaaca tcaccttttc
     3421 ggtcgcaccg ggaacggact tccgcacgct ggatctgaac aaacttcgct tctggctcgg
     3481 caatgacgac aactataccc gtgaccagct ttatttatgg ttctgcgaat acttgcaggg
     3541 tgccgacctg actgtgggtg aacagcatat tcgcctgcct gagtttatgc taaaagctgt
     3601 cggttttgag ccgcaggatg ccatgctgcc ctggccgaaa aacgtccaca gcggctaccg
     3661 gatccttcag gagtatttct gttaccccga tgcgtttctc ttttttgatc tttgtggttg
     3721 tccggctttg cctgacggat tgcaggcgga attctttacc ctgcaactgc gtttttcgcg
     3781 ccctttgccc gtggacatcc ggctgcgccg cgattccctg cgcctgtatt gcgcacctgc
     3841 cattaattta tttatccacc atgcagaagc catcacgctg gacaaccggc gggcagacta
     3901 tccgctggtt cccagccgcc attacccaca acattacgat gtattttccg ttaacagtgt
     3961 ggtgagccag gtccaggata tgttcaggaa aaaagatctg gggcgtcctg tttcgacgca
     4021 ggccgcgcgc cagtggccag cctttgaaag tttcagccat cagatggaat acagccggaa
     4081 gcgggaggtg gtgtactggc atcaccggac caaaacatcc ctgttccatc gcggctttga
     4141 tcataccctt gcctttatac atgctgatgg cagttatccg tcagacgaat ctctgctcag
     4201 taatgaagtg gtttcggtat cgctgacctg taccaaccgt gagcttccgt cacaaattcg
     4261 ttccggcgat atcaccggca caaccggtaa aaatgcggct gttgcttcct ttcgcaacat
     4321 tacccgcccg acgcaaccac tctggccggt cattgatggc agcctgcact ggtccctgct
     4381 ctccgccatg aacctgaatt atctgtcatt actggatacg gacgcgctga agcaggtcat
     4441 cgccaacttt gatcgccacg caatccacca tccgcagacg gcgcggctgt cacaacaaaa
     4501 gctggatgcc attgagcgtc tggagacccg ccccgttgat cgcctgttta cgggtattcc
     4561 cgtccgggga ctggcctcca cgctgtatct gcacccggag ccgtttgtct gtgaagggga
     4621 aatgtatctg ctcggtacgg tgctttcgca ttttctgtcg ctgtacgcca gcgttaactc
     4681 attccacatg ctgaccgttg tgaacacaga aagccaggag acatggaaat ggacggaaag
     4741 aatcgggcag catcctctta tctgagtccg ggaaatcccc cggcagataa agagcagaat
     4801 gatccgctgg cacaggtttt ccataatgcc tgctcttaca atttttttgc gatggcggag
     4861 ctgttgcacc gcctggcaaa gggtgaaaag gggacgccag aattatccct gcgcgacgat
     4921 ccggcacagg aaaccctgcg tttcagcgcc gatgccagcc ttgccttccc gtgcagtgat
     4981 atcagtgcgc tgaaacggga tacgtcaggg gcattcagga tgaccactac ctttatgggg
     5041 ttacagggga gccagtcgcc tctccccggt tattacctcg atcacctggc ctggaaagcg
     5101 gtacatgaac aaagtccggt aggtgatttt ctggatatgt tcagccaccg cctgacccag
     5161 tttgtctggc atatctggcg taagtaccgc tatcacatca gttttcgtaa cggcggtgtg
     5221 gatgcgttct cgcagcgcat gtactccctt gtcgggctgg gccaccgcca gcttcgcgat
     5281 aagctggcga ttaatcacag caaaatgctg gcgtattccg ggatcctggc aaatccggga
     5341 cgatccccgg aaattatctg tggcctggtg tcccactgct ttgatttgtc agaagtgacg
     5401 ctgcaaaact ggcaacgtcg caaggtggat attgaaccgg atcagcaaaa cagcctgggg
     5461 agctattctc ttaaaaatgg cgaaaaactg gcgggaagat cggtactggg caactttgtt
     5521 ctgggtacac gagtacccga tctcagcgga aaattccagc tcagtattac cagcctgacc
     5581 cgaaaacagt tcctctcttt tctgccgtcc ggcgaaaact tcctgccact gacgacgttc
     5641 gtgtccttca tcctgcgcga tcagcttgcc tgggacttac atctggggct ggcgccggag
     5701 caggtgggcg caatgcgcct gggcgataac aaaagtgcgc tgctgggctg gaccagcttc
     5761 ctcggcacac cggaagaacg accctcagtc accatcaggg tacggtcgta aattatgata
     5821 atcacggagc agaatatgcc ggaagagaaa ctacagacgc tttcattgca ggtcattaac
     5881 ggcagtgagc tggaaagcgg gcgggcggcg cgctgtctgt tcacacagca gggaaatgtc
     5941 ggccatgccc ccgaatgcca ctggtcggta caggatcgtc agcagagcat tccggcccag
     6001 gcttttaccg ttatcctgca cgatggcaca ttttgtctac gcccgcagac ggcacaactg
     6061 tggctgaatc aggcaaaagt cacagcaaca tcagacctga tacagttgcg ccagggcgat
     6121 gagatccaga tcggacggct gatggtgagg gttcatctga accggggaga tattccccat
     6181 tacgatgagg aaatggccac tcccgaaacc atcgttacca atcgcgatat gctcacggat
     6241 accctgctat caacggaggg tgcgccacac tatccgggaa tgactcaccg gcaccagctt
     6301 gcagacaccg tggtaaatgg tttttctgcc gatccactcc aggcacttca gtccgaaagc
     6361 ctgattacca cgggcgatcc gctttcaggc attgcggctg tccggccatc ggcaccgctg
     6421 tccgatccgg caagtaatgg ggggatcaat actccgttta tggatctgcc gcccatttat
     6481 gccagccctg gcgatcataa tgatgacatc tctgcggcag aaatggcgca acgccacctt
     6541 gcggtcaccc ccttactgcg cggtcttggc ggctcgctta ccgtgagcaa ttccgacgat
     6601 gcggatgatt ttctggagga ggccggacga acgttacagg ccgcaataaa aggtctgctc
     6661 gatttgcagc agcagcgtaa cagcctctca gacaaacatt tgcgcccgct ggaagataac
     6721 ccgctgcgcc tgaacatgga ttacgccacc gcgctcgacg tgatgtttgc cgaaggtaaa
     6781 agcccggtac atctggcggc tcccgccgcc gtcagtgaaa gcctgcgcaa tgtccgccac
     6841 cacgaagaag ctaaccgggc ggcgattgtg gagtcgcttc gtgtcctgct ggatgctttc
     6901 tcaccacaaa atctgctgcg ccgctttgtg cagtaccgcc gcagccatga actgcgccag
     6961 ccgctggatg atgccggagc atggcaaatg tacagccatt attacgaaga actggcctcc
     7021 gatcgccagc aggggtttga gatgctgttt aacgaggtct acgcccaggt ctatgaccgg
     7081 gtgcttcgtg aaaaacagcg ggagccggaa gcatgaacag gaaggcgttt ctggcctgcg
     7141 tgctgatgtg cgtcatatta accggctgcg aaacggcgaa aaaaatcagc gaggtgatca
     7201 aaaacccgga tatccaggtc gggagcctga aggagcaacc ttcggaaata accgtcaccc
     7261 tgctgactga accggacacc aataccaatg cggagggtga atcagccgcc gtggatgtgc
     7321 aactggtgta cctgactgac gactcaaaat tgcaggcagc cgactatgac cagatagcca
     7381 gtacgccgct accggatgtg ctgggtaaaa actatatcga ccaccaggat ttcaacctgt
     7441 tgccggacac catcaaaacc ctgccgccga taaagctgga tgagaaaacg cagtttattg
     7501 gtgtcgtggc ctatttctcc gatgaccagg ccactgaatg gaaacaaatc gagacggtgg
     7561 aaggaaccgg ccaccattac cgcctgctgg tgcatgtacg tcaaagcagc atcgaaatga
     7621 aaaaagagga tgaataatta tggcaaccac ccgcaacaag gtgatgtggc aggaagggat
     7681 gctgatgcgc ccacaccatt tccagcaaca gcagcgttac aacgactacc tggataacca
     7741 gcgtttccgg gccatgaatg atttatcctg gggatttacc gaactcaccc tcaacaatga
     7801 actgctggcg cagggtaaga tcatgattga cagcgcgtca ggcacactgc ctgacggcac
     7861 cgtcttttct atccccgacc aggacgcact gcccgatccg ctgcacccgc aaaattttcc
     7921 ggacgaaaga agccgcaata tttacctcgc tctgccggtc gccagtgatg tgagaaatga
     7981 aatcagcgac gggcggcgaa tcgggcgtta ccggctgaat tatgccgatg tccgggatct
     8041 gcattcagaa gaaggcgacg cgcgaacact gacgctggga caactgacgc cgcgcattat
     8101 gagcggtgca gaagatatga gtgcctatat tacgctcccg ctttgccgta tcagcgatcg
     8161 ccatgccgac ggctccctga cgctggatga cgattttatc ccctcctgcc agaatattca
     8221 ggtcagtaag aaactgcgtg tttatctcaa agaggtacag ggggccattg gcggacgggc
     8281 aagcgatctg gcaaaccgta ttggctctcc ggcgcagagc ggcatcgcgg atgtggcgga
     8341 atttatgatg ttgcagttgc ttaaccgtaa ccagacccgg tttacccatc gcgcccgccg
     8401 atcccagctc cacccggaag atttctacct tgatcttgcc gagttgctgg gtgaactgat
     8461 gacctttaca gagccgtcac gcctgccctg cccgcttgat gtgtatgatc atcatgacct
     8521 gaccaaaaca tttaaaacac tgttaccgga agtcaaacgg gcgctgcata ccgtactgtc
     8581 gccaagagcg gtcaatctgc cgctgcatct gcgtgacggc atctggcagg ccgatgttca
     8641 tgactcggaa ctgctacagt ctgccacctt tgtactggcc gtggcagcaa atatgcctgt
     8701 cgatcagatc cagcgtcagt ttatccagca gtcgaaaatt tcctcgccgg aaaaaatccg
     8761 caatatggtc agtgtgcaga tcccgggtat tcctctgcgc gcactgatgg tggccccccg
     8821 ccagcttccc taccattccg ggttcagcta tttcgaactc gacaagagcg gccaggcctg
     8881 gacagaaatg gctgccgccg gggccgttgc actgcatgta tccggcagtt tcccggatct
     8941 gaacatgcaa ctgtgggcga taagagggta agacgatgga tgaggggtca ctgtctctgc
     9001 caccatttac cggatacgac gaaaaaagcc tgcgcgacta ccatctcgcg cttcatggca
     9061 acagcctgaa tccgatgatc gacgcggcga cgccgctact cggtatggta atgcgccttt
     9121 ccaccatgaa cagccagacg atgccggagc atctttttgc tcaggtggtg acagatgttc
     9181 aggccgtgga gcaactgctt caggaacagg gctacgaacc gggcgtgatt atctcgtttc
     9241 gctacatcct ctgcacgttt attgatgaag cggcgctcgg taacggctgg tcgaacaaga
     9301 acgagtggat caagcagtca ttgctggtcc atttccacaa cgaagcctgg ggtggcgaga
     9361 aagttttcat cctgctggag cgcctgatcc gtgaacccaa acgttaccag gatctgctgg
     9421 aatttctctg gctctgtttt tctctgggat ttcgcgggcg ctacaaagtg gcagtacagg
     9481 atcaggggga atttgagcag atctaccgcc gtctgtacca cgttctgcat aaactgcgcg
     9541 gcgatgcccc tttcccgctg ttacaccagg acaaaaaaac ccaaggcggg cgttatcagc
     9601 tcatcagtcg cctgactgtt aaacatattt tttgtggtgg cgttgtcgtg ctggcgttgt
     9661 tttacctgtt ctatctgttg cggctggaca gccagacgca ggacatcctg caccagctaa
     9721 acaaattact tcgataagga cgtttatgat ccagattgat cttcccacgc tggtaaaacg
     9781 gctgaacctg ttctcccgcc aggcgctgga gatggccgcc tcagaatgta tgagccagca
     9841 ggcagcggaa attaccgtca gccatgtgct tattcagatg ctcgccatgc cacgcagtga
     9901 cctgcgggtt attacccgcc agggcgatat tggcatggaa gagttgcgcc aggcgttgac
     9961 ggtagagaac tacacaaccg cccgttctgc ggacagctac ccggcgtttt ccccgatgct
    10021 ggttgagtgg cttaaagagg gctggctgct ggcgtcggct gagatgcagc acagcgaact
    10081 gcgcggcggc gtgttgctgc tggccttgct gcattcgccg ctgcgttata taccgcctgc
    10141 tgccgcccgg ctgttgaccg gcattaaccg tgaccgtctg caacaggact ttgtgcagtg
    10201 gacacaggag tcggcggaat cagtcgtgcc ggatgcagac ggtaaaggcg caggcacact
    10261 gacggacgcc tctgacaccc tgcttgcccg ctacgccaaa aacatgacag aagacgcccg
    10321 taacggcagg ctagccccag tactgtgccg cgaccacgaa atcgacctga tgatcgacat
    10381 tctctgccgc cgccgtaaaa acaacccggt ggtggtgggc gaagcaggcg tgggtaaaag
    10441 cgcgctgatt gaagggctgg cgctgcgcat cgtggcaggc caggtgccgg acaaactgaa
    10501 aaacaccgat atcatgaccc ttgacctggg cgcattgcag gccggggcat cggtgaaggg
    10561 tgagtttgaa aaacgcttca aggggctgat ggcggaggtc atttcctctc cggtgccggt
    10621 cattctgttt atcgacgaag cgcataccct gattggcgcg ggcaaccagc agggcgggct
    10681 ggatatctcc aacctgctga aaccggcgct ggcgcgtggc gaactgaaaa ccatcgccgc
    10741 caccacctgg agcgagtaca aaaaatactt cgagaaagac gccgccctgt cgcgccgctt
    10801 ccagttagtg aaggtcagcg aacccaacgc tgccgaagcc accattattc tgcgtggtct
    10861 gtcggcggtc tatgaacggt ctcacggcgt gctgattgac gatgatgcct tgcaggccgc
    10921 agcaacgctg agcgaacgtt atctctccgg gcgtcagtta ccggacaaag cgattgatgt
    10981 gctggatacc gcctgcgccc gtgtggccat caacctgtcg tcgccgccga agcaaatctc
    11041 ggcgctgacc actctgagcc accagcagga ggcggaaatt cgccagcttg agcgcgagct
    11101 tcgcatcgga ctgcgtaccg acacatcacg gatgaccgag gtgctggtgc agtatgatga
    11161 aacgctgacg gcgctggatg aactggaagc ggcttggcac cagcagcaga cgctggtccg
    11221 ggagattatc gcgctgcgcc agcagttact gggcgtggca gaggacgatg cggcgccgtt
    11281 gccggacgca gatactgtgg aggatacgca gccagagtca gagtcagagt cagaacagga
    11341 taataccggt gccgtaccgg ctgatgagac cgacagagaa cagccggaag agaccgctga
    11401 aacagtttcc ccggtacagc ggctggccca gctcactgcc gaactggacg ccctgcataa
    11461 cgaccggttg ctggtctccc cgcacgtcga taaaaaacag attgcggcgg tgattgccga
    11521 atggaccggc gtgccgctca accgcctgtc gcagaatgaa atgtcggtca tcaccgacct
    11581 gccgaaatgg ctgggcgaca ccatcaaagg ccaggacctg gcgattgcca gcctgcataa
    11641 gcatctactg accgcacgcg ccgacctgcg tcgtccggga cgcccgctcg gtgcgttcct
    11701 gctcgctggc cccagcggcg tgggtaaaac cgaaaccgtc ctgcaactgg cagagctgct
    11761 ctacggcggt cgccagtacc tgaccaccat caatatgtcc gagttccagg agaaacacac
    11821 cgtttcgcgg ctgattggct cccctccggg ctatgtcggc tatggcgaag gcggcgtgct
    11881 gactgaagcc atccgccaga aaccctactc ggtggtgctg ctcgatgaag tggaaaaagc
    11941 gcacccggat gtcctcaacc tgttctacca ggcgtttgac aagggcgaga tggccgacgg
    12001 cgaaggccgc ctgattgact gtaaaaacat cgtcttcttc ctcacctcca acctcggtta
    12061 ccaggtgatt gtcgaacacg cggatgaccc ggaaaccatg caggaagccc tctatccggt
    12121 gctggcggac ttctttaaac ctgccctgct ggcgcgaatg gaggtggtgc cgtacctgcc
    12181 gctgtcgaaa gagacgctcg ccaccattat cgccgggaaa ctggcccgtc tggataacgt
    12241 gctgcgcagt cgctttggtg cagaagtggt cattgaaccg gaagtgacgg acgaaatcat
    12301 gagccgcgtc acccgcgcgg aaaacggcgc gaggatgctg gaatctgtca tcgatggcga
    12361 catgctaccg ccgctctcgc tgctgctgtt gcagaaaatg gcggctaaca cggcgattgc
    12421 ccggattcgg ttgtcggcag tggacggcgc atttacggca gacgtggaag atgctcagaa
    12481 cgacgagtcc gtcacaaagg atgaaacggt tttatgagca ggttgcgcgt tttccggcag
    12541 acaggatggt ttatcgcggg cctgatgacg ggcttacccg ccactgccgc gcccgcagaa
    12601 gcatcgtcaa tggccggtgt ggcggtcgcc gtcgccacca cgaccccgcc ggatgcgaca
    12661 gccacgttac aggccatgca gtcctgtcgg cgggagtccg cggcgctgga gcggcttgac
    12721 tgctatgacc acctgctggc accgctgtcc ccgtcagggt ttgacggggc gctggtcaaa
    12781 gccggttttg tgggcgaggc ctggacccgc gccacagagc aggaaaaacg ccgcgagggc
    12841 aacaccacag aactgctggt gacgcaggtg ccgggggagc gcccgacggt ggtgataacc
    12901 accccggcta tcggccatgt tccgccgcgc ccggtgctga tgttcagttg cgtggacaat
    12961 atcacccgga tgcaggtggc gctgatgcac cctctggatg tccatgatat cgccgtcact
    13021 ctgaatgccg acagccgggc attgcgcagc cactggtttg tgcgcgagaa cggcaccctg
    13081 ctggagtcca gccgggggct gtccgggatt gatgaaatta agcaactgtt cggcgcaaaa
    13141 acgctgacag tggataccgg cgcagataac gccgcaggaa agctgacctt taacattgac
    13201 gggctggcaa gggcgattgc ccccctgcgc gatgcctgcc actgggcggg agaataaaaa
    13261 tatggcaatg gacttacgcg atccgaatgt ctggatatcg cacctgctgg aaaacctgcc
    13321 ggaagaaaaa ctggcatcgg cgctgaaaga tgacaacccg gactgggagt atatcgacgg
    13381 cgaaatcgtc aagctggggt ctctggccca cgctcagctt gatattcccg aactacaacg
    13441 cagggggcta cagcttctgg cttctgaaag caaagacttc aggctactgg cacacctgct
    13501 cagaaccctg caacatgccg gtgatccact gctggcactg cacctgctaa cgctatacgt
    13561 ggaacattac tggactgtgg ccgcgccgca gaatatggcg cataaaaaac gctttgccag
    13621 ccagatcatt aaacgttttg aaacgggtat tgaaggcttt tcacaaaacg ctgccacaac
    13681 gcagcgcgat actctgctgg gtgagctggc gaaactggcg cagtgctggc agtcacataa
    13741 cgtcccggaa ctggcacagg ctaccgatga tctttttgcc ctgtaccagc gtacgtttaa
    13801 tcgtgcggct cctgctccgg tccccactcc ggcggcctcc ggtagttcac cacaaaccac
    13861 cgtcacgtct gaaagcggcg tgacgcaacc cagtgctccg gctccccaaa tcgccatcga
    13921 cagtcacgac gacaaagcct ggcgcgacac gctgttaaaa gtggcggcta ttttatgtga
    13981 acgccagccg gactcgccgc agggctatcg cctgcgccgc catgccctgt ggcaatccat
    14041 caccagtaca ccccaggcgg aaagcgatgg acgtacccca ctggctgcgg tctctgccga
    14101 tatggtggcg gattaccagt cccggcttgc cagcgcggat atggcgctgt ggcaacaggt
    14161 tgagaaaagc gtattgctgg ctccttactg gctggacggt cactgtcttt ctgcacagac
    14221 ggcactgcgt ctgggttaca aacaggtggc agacaccatc cgcgatgagg tcatccgctt
    14281 ccttgagcgt ctgccccagc ttaccgggct gctgtttaat gaccgcacac cgtttctcag
    14341 tgagcagacg aaacaatggc tggctgcttc gcccgacggc aaagttgcac cggttgcgca
    14401 aatcggtgag gaatcgcagg cagccagagc ctgttttgct gggcagggtc tggaggcggc
    14461 gctgcgatat ctggacatgc tacccgaagg cgatccccgc gatcagtttc accgccagta
    14521 ccttgccgca cagttgacgg aggaggcggg gctgatacag cttgcgcagc aacagtaccg
    14581 gatgttgttg atgataggga gtcagatgat ggtgtctgac tgggagccat cattacttac
    14641 gcagcttgaa caaaaattca cggcagaaca ataaaagaga acaggagttt ttgtgttcag
    14701 attaccaaca ccccgactac tcagcggact caaatcagcc ctgcgaccgg cgatgccccg
    14761 gtttaaagtc tccgctttct ggctgctgat actggcgtgg atttttctgc ttgctgatac
    14821 tggcgtggat ttttctgctg gtatggatct ggtggaaagg cccgacgtgg acgctgtatg
    14881 aagaacagtg gctcaaacca ctggcgaacc gctggctggc aacggcggcg tgggggatta
    14941 ttgccctggt gtggcttacc gtccgggtaa tgaagcgcct gcaactgctt gaaaaacagc
    15001 aaaaacagca gcgcgaagag gccattgatc cgctcagtgt ggagctgaac gcccagcagc
    15061 gttaccttga ccgctggctg ctgcgcctgc aacgccatct cgacaaccgc cgtttcctgt
    15121 ggcagttacc gtggtatatg gtcatcggcc cggcgggcag tggcaaaacg acactgctgc
    15181 gcgaggggtt tccgtcagac attatttatg ccccggaggg cgcgcgcggc acagaacaac
    15241 gcctgtacct tacgccccat gtcggtaaac aggcggtgat ctttgatatc gacggcacac
    15301 tctgcgctcc cgctgatgcg gatatcctgc atcgccgtct gtgggaacat gccctcggct
    15361 ggctgaaaga aaagcgcgcg cgccagccgc tgaacgggat tattctgaca ctcgatttac
    15421 ccgatctgct gaccgcagac aaacgccacc gcgagcatct gttacagacg ctgcgcagcc
    15481 gcttacagga tatacgccag catcttcact gccagttacc ggtttacgtg gtactgaccc
    15541 ggcttgattt attacagggt tttgccgccc tgttccagtc cctgaacaga caggatcgcg
    15601 atgcgattct gggcgtcacg ttcacccgcc gtgcccatga aaatgatgac tggcgaacag
    15661 agttgaatgc tttctggcag acatgggtgg atcgaatgaa tctggcgttg ccggatctga
    15721 tggtcaccca gactcacacc cgcgcgtctt tattcagttt ttcccgccag atgcagggaa
    15781 gccgtgaacc gctggtgtca ctgcttgagg gtctgcttga cggtgaaaat atgaacgtga
    15841 tgctgcgtgg tgtctatctc acctcttcgc ttcagcgcgg gcagatggat gatatattca
    15901 cccagtctgc cgcccgccag taccgactgg gcaataaccc actggcgtcc tggcctctgg
    15961 tggacaccgc gccttatttc acccgcagct tgttcccgca ggcattactc gcagagccta
    16021 atctggcaac ggagagccgc gcctggctga tgcgttcccg tcgccgcctg acggttttct
    16081 ctgccaccgg cggcgtggcg gcactgctgc tcatcaccgg ctggcatcac tattacaacg
    16141 gtaactatca gtccggcatc accgtgctta agcaggccaa agcctttatg gatgtgccgc
    16201 ctccacaggg ggaagatgac tacggcaacc tgcaactgcc gctgctgaac ccggtacgcg
    16261 atgccacact ggcctatggc gactggggcg accgcagccg tctggccgat atgggactgt
    16321 accagggacg acgtatcgga ccttatgtgg aacagaccta tctgcaactg ctggagcaac
    16381 gttacctgcc ctcgctgttt aacgggctgg ttaaagcgat gaatgccgcg ccgccggaga
    16441 gtgaagaaaa actcgcggtg ctgcgcgtga tgcgaatgct ggaggacaaa agcggacgta
    16501 acaatgaggt ggtgaagcag tatatggcaa aacgctggag cgaaaagttc cacggccagc
    16561 gcgatatcca ggcacaactg atgtcccatc ttgactacgc gctggctcat actgactggc
    16621 acgcagagcg tcaggcgggc gacggtgacg ccatcagccg ctggacgcca tatgacaagc
    16681 ccgtggtatc agcacagaaa gaactgagca aactgcctgt ctaccagcgg gtttaccaga
    16741 gcctgaaaac gcgggcgctg ggcgttcttc ctgccgacct caatctgcgt gaccaggtag
    16801 ggccaacctt tgaccaggtg tttacatctg ccgatgacaa caaactggtt gttccacagt
    16861 ttcttacccg ttacggcctg caaagctatt ttgtaaaaca gcgcgatgaa ctggttgaac
    16921 tgacggcgat ggattcctgg gtacttaacc ttacccgcag cgtgaaatac agtgacgccg
    16981 accgcgcgga aatccagcgc cagctcaccg agcagtatat cagcgactac accgccacct
    17041 ggcgggccgg gatggacaat ctgaatatcc gcaattttga gtccatcgga caactgaccg
    17101 gggcgctgga gcaggttatc agcggcgacc agcctttgca gcgggcgctg accgtgctgc
    17161 gtgacaacac acagccaggc gtcttttctg aaaaactctc tgccaaagaa cgggatgagg
    17221 ccctggcaga gccggactac cagttactca cccgtctcgg gcatgagttc gccccggaaa
    17281 acattaccct ggcagtacag aaagacaaag aaagcacgat gcaggccgtg tatcagcaac
    17341 tcaccgagtt gcaccgctac ctgctggcaa tccagaacgc gcctgtacca gggaaatcgg
    17401 cgctgaaagc cgtgcagtta cggcttgatc agaacagcag cgatccgata ttcgccaccc
    17461 gccagatggc aaaaacgctg cctgctccgc tcaaccgctg ggttggcaga ctgactgacc
    17521 aggcctggca tgtggtgatg gtggaggctg tacattatat ggaagtggac tggcgcgaca
    17581 gcgtggtgaa accgtttaac gagcaactgg caaataacta tccgtttaat ccgcgttctg
    17641 cacaggatgc ctcactggat gccttcgaac gcttctttaa accggatggc atactggata
    17701 ccttctacca gcagaacctg aagctgttta tcgataatga cctgagtctg gaggatggcg
    17761 ataacaacgt cattattcgc gaagatatta ttgcgcaact ggaaactgcg cagaaaatcc
    17821 gtgacatctt cttcagcaaa cagaacggtc tgggaacatc ctttgccgtg gaaacggtat
    17881 cgctttcagg caataaacgc cgcagtgtac tgaaccttga cggtcagtta gtcgattaca
    17941 gccagggccg taactatacc gcccatctgg tctggcctaa caacatgcgc gaaggcaacg
    18001 aaagtaagct gacgctcatc ggcaccagcg gcaacgcgcc gcgcagtatc agcttcagcg
    18061 ggccgtgggc gcagttccgc ctgttcgggg ccggacaact gaccggagta caggatggca
    18121 actttaccgt gcgctttagc gtggacggtg gcgcgatgac ctaccgtgtg cataccgaca
    18181 cggaagataa cccgttcagc ggtgggttgt tcagccagtt tggtctgtca gacacactgt
    18241 actgaggagg cagggatgaa tagtaacgta ctgacacaaa ctatcgttac cggcagtgac
    18301 ccgcgcgggc tgccggaatt cagcgccatc cgcgaggaaa taaacaaagc cagccacccg
    18361 tcacagcctg agctgaactg gaaactggtg gagtcgctgg cgctggcgat ttttaaagcc
    18421 aacggtgtgg atttacacac cgccacctac tatacgcttg cccggacacg gacacaggga
    18481 ctggcgggat tctgcgaagg tgcggaactg ctggcggcaa tggtaagcca cgactgggat
    18541 aagttctggc cgcagggcgg cccggcgcgt actgaaatgc tggactggtt taactcccga
    18601 accggcaata ttctgcgtca gcaaatctcc tttgcggaat ccgacctacc actgatctac
    18661 cgcacagagc gggcattgca gcttatctgc gataagctcc agcaggtgga actgaagcgc
    18721 gttccgcgcg tggagaatct gctctatttt atgcagaaca cgcgtaaacg gcttgaaccc
    18781 cagctgaaga gtaacactga gaacgccgca cagaccacgg tcagaacgct gatttatgcc
    18841 ccggaaacac aggcatcttc cacaccagaa gcggtagtgc ctcccctgcc cggcctgcct
    18901 gagatgaaag tggaagtgcg cagtctgaca gagaatcccc cacaggccag tgtgataaag
    18961 caaggcagta cggtaagagg gtttatcgca gggatcgcct gttcagtggc tgtcgcctca
    19021 gcattgtggt ggtggcaggt ctatccggtg cagcagcaac tgttacaggt taacgacacc
    19081 gctcagggcg cagcaacggt gtggatggcc tcacctgaac tcgaaaacta tgagcgcagg
    19141 ctgcaacaac ttcttgatac ctccccggta cagccgctgg aaaccgggat gcagatgatg
    19201 cgtgttgccg acagtcgctg gccggaaagc ctgcaacagc aacaggcctc gacacaatgg
    19261 aatgaggcac tcaaaacccg cgcacagagt agcccgcagt tgcgtggctg gttgcagacc
    19321 cgccaggact tacatgcttt tgcagatcta gtgatgcagc gcgagaaaga gggactaacc
    19381 ctttcctata tcaaaaatgt catctggcag gcggagcggg gactggggca ggaaacaccc
    19441 gttgagtctc tgttgacgca gtaccaggat gcccgtgcgc agaagcagaa tacagatgcg
    19501 ctggaaaaac aaattaatga gcgactcgaa ggcgtgttaa gccgctggct gctgctgaag
    19561 aataatacga taccgacgat aaaaaaagca ttgaatttca ataacattca tgaatacaaa
    19621 ggagttttaa atggcgaatt taatttattt aacactaaat ggtgataatc aagggctcat
    19681 ttcaagtggt tgttcatcac aaccatccat tggaaataaa gctcaaacag cacacattga
    19741 tcaaattatg atttatgaat tcatgcatgg attaaatcga gatcaaaacg tcaaccatca
    19801 tcatttgaca ataaaaaa
//
LOCUS       DBFIFM010000075        19038 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.075, whole genome shotgun sequence.
ACCESSION   DBFIFM010000075 DBFIFM010000000
VERSION     DBFIFM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.075"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(153..1802)
                     /gene="pgi"
                     /locus_tag="N7C74_002320"
     CDS             complement(153..1802)
                     /gene="pgi"
                     /locus_tag="N7C74_002320"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HGU2432181.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2039..2235
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2327..3676
                     /gene="lysC"
                     /locus_tag="N7C74_002321"
     CDS             2327..3676
                     /gene="lysC"
                     /locus_tag="N7C74_002321"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HGU2432182.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            3929..4201
                     /locus_tag="N7C74_002322"
     CDS             3929..4201
                     /locus_tag="N7C74_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HGU2432183.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4202..5074)
                     /gene="rluF"
                     /locus_tag="N7C74_002323"
     CDS             complement(4202..5074)
                     /gene="rluF"
                     /locus_tag="N7C74_002323"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HGU2432184.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5422..6369
                     /locus_tag="N7C74_002324"
     CDS             5422..6369
                     /locus_tag="N7C74_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HGU2432185.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTNISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGEMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            6439..7245
                     /locus_tag="N7C74_002325"
     CDS             6439..7245
                     /locus_tag="N7C74_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU2432186.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEIADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7255..7662
                     /locus_tag="N7C74_002326"
     CDS             7255..7662
                     /locus_tag="N7C74_002326"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HGU2432187.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQNEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            7662..8156
                     /locus_tag="N7C74_002327"
     CDS             7662..8156
                     /locus_tag="N7C74_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HGU2432188.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIYKINEQ
                     LIAN"
     gene            8222..9019
                     /locus_tag="N7C74_002328"
     CDS             8222..9019
                     /locus_tag="N7C74_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HGU2432189.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLLAPASTALDQLDD"
     gene            9030..9854
                     /locus_tag="N7C74_002329"
     CDS             9030..9854
                     /locus_tag="N7C74_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGU2432190.1"
                     /translation="MEQRKITRGDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPVKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            9906..11135
                     /gene="sorE"
                     /locus_tag="N7C74_002330"
     CDS             9906..11135
                     /gene="sorE"
                     /locus_tag="N7C74_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HGU2432191.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            11518..12207
                     /gene="pepE"
                     /locus_tag="N7C74_002331"
     CDS             11518..12207
                     /gene="pepE"
                     /locus_tag="N7C74_002331"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HGU2432192.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12298..13929)
                     /locus_tag="N7C74_002332"
     CDS             complement(12298..13929)
                     /locus_tag="N7C74_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HGU2432193.1"
                     /translation="MLTLLNLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLAMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMSSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14149..17832)
                     /gene="metH"
                     /locus_tag="N7C74_002333"
     CDS             complement(14149..17832)
                     /gene="metH"
                     /locus_tag="N7C74_002333"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HGU2432194.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18032..18856
                     /gene="iclR"
                     /locus_tag="N7C74_002334"
     CDS             18032..18856
                     /gene="iclR"
                     /locus_tag="N7C74_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HGU2432195.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         4481 a         4967 c         5013 g         4577 t
ORIGIN      
        1 gtgctatgca ttaattgccg aatacgatgt acatatcggc atcgacctgt aggcctgata
       61 agacgcgtaa agcgtcgcat caggcaccag ttgccggatg cggcgtaaac gccttatccg
      121 gcctacatag cggcatcagg catgaacgat gattaaccgc gccacgcttt atagcggtta
      181 atcagaccat tggtcgagct atcgtggctg ctgatttctt tatcatcttt cagctctggc
      241 agaatacggt tcgccagctg tttacccagt tccacgcccc actggtcgaa ggtgaagatg
      301 ttcaggatca cgccctgagt aaagattttg tgctcataca gcgcaatcaa cgcacccagg
      361 ctgaacggag tgatttcgcg caacaggata gagttggtcg ggcggttacc ttcgaacact
      421 ttgaacggca ccacgtagtc aagcgttgcc ggatctttac cctgatcgcg atattcctgc
      481 tcaaccactt cgcgggattt accaaacgcc agcgcttcgg tctgggcgaa gaagttagac
      541 agcagtttct ggtggtgatc agagagcggg ttatgggtga tagccggagc gatgaaatcg
      601 cacggtacca ttttggttcc ctggtggatc agctggtaga acgcgtgctg accgttagtg
      661 cctggttcac cccagataat cgggccagtc tggtaatcca caacgttacc gttacggtca
      721 acatacttac cgttggactc catattgccc tgctggaagt acgccgcgaa acggtgcata
      781 tactggtcat acggcagaat cgcttcagtt tccgcaccaa agaaattgtt gtaccagatg
      841 ccaatcagcg ccaacagtac aggcaggttt ttctcggcag gcgtggtgga gaaatgcttg
      901 tccatcgcgt gtgcgccaga aagcagttca acgaagttat caaagccgat ggagagaaca
      961 atcgacaggc caatcgctga ccacaaagag taacgaccgc cgacccagtc ccagaactcg
     1021 aacatgttgg cagtatcaat accaaactcg ccaacggctt tggcattggt tgagagcgcc
     1081 gcaaagtgtt tcgcaacgtg tttttcatca cctgccgctt tcaggaacca gtcacgcgcg
     1141 ctatgggcgt tggtcatggt ttcctgagtg gtgaaggttt tagatgctac caggaacagc
     1201 gtggtttccg ggtttacttt tttcagcact tcagcgatgt gagtcccatc gacgttagaa
     1261 acaaagtgca tgttcaggtg atttttgtac ggacgcagtg cttcggtcac catgtacggg
     1321 ccgaggtcag aaccgccgat cccgatgttc actacgtcag tgattgcttt gccggtataa
     1381 cccttccact caccggagat aatcgcttcc gagaaggttt tcatcttctc cagcaccgcg
     1441 ttgacttctg gcataacatc tttgccatca accagaatcg gggtattgct acgattacgc
     1501 agcgctacgt gcagcacggc gcggttttca gtgcggttga tcttctcgcc agagaacatc
     1561 gacttaatcg cgcccgccag atcgcactct ttcgccagat cttgtaattt cgccagcgtc
     1621 tcttcagtga tgcggttttt ggagtaatcc accagcatct gatcgtcgaa ggttgcggag
     1681 aacttagaaa aacgatcgcc gtctttagca aaaagatcgg cgatcgtaac gtctttcatt
     1741 tcatcgaagt gtttctgtag tgcctgccag gcagcggtct gcgttggatt gatgtttttc
     1801 attagcaata ctcttctgat tttgagaatt gtgactttgg aagattgtag cgccagtcac
     1861 agaaaaatgt gatggtttta gtgccgttag cgtaatgttg agtgtaaacc cttagcgcag
     1921 tgaagcattt attagctgaa ctactgaccg ccaggagtgg atgaaaaatc cgcatgaccc
     1981 cgtcgttgac aaccgccccg ctcacccttt atttataaat gtactacctg cgctagcgca
     2041 ggccagaaga ggcgcgttgc ccaagtaacg gtgttggagg agccagtcct gtgataacac
     2101 ctgagggggt gcatcgccga ggtgattgaa cggctggcca cgttcatcat cggctacagg
     2161 ggctgaatcc cctgggttgt caccagaagc gttcgcagtc gggcgtttcg caagtggtgg
     2221 agcacttctg ggtgaaaata gtagcgaagt atcgctctgc gcccacccgt cttccgctct
     2281 tcccttgtgc caaggctgaa aatggatccc ctgacacgag gtagttatgt ctgaaattgt
     2341 tgtctccaaa tttggcggta ccagcgtagc tgattttgac gccatgaacc gcagcgctga
     2401 tattgtgctt tctgatgcca acgtgcgttt agttgtcctc tcggcttctg ctggtatcac
     2461 taatctgctg gtcgctttag cggaaggact ggaacctggc gagcgattcg aaaaactcga
     2521 cgctattcgc aacatccagt ttgccattct ggaacgtctg cgttacccga acgttatccg
     2581 tgaagagatt gaacgtctgc tggagaacat tactgttctg gcagaagcgg cggcgctggc
     2641 aacgtctccg gcgctgacag atgaactggt cagccacggc gagctgatgt cgaccctgct
     2701 gtttgtcgag atcctgcgcg aacgcgatgt tcaggcacag tggtttgatg tacgtaaagt
     2761 gatgcgtacc aacgaccgat ttggtcgtgc agagccagat gtagccgcgc tggcggaact
     2821 ggccgcgctg cagctgctcc cacgtctcaa tgaaggctta gtgatcaccc agggatttat
     2881 cggtagcgaa aataaaggtc gtacaacgac gcttggccgt ggaggcagcg attatacggc
     2941 agccttgctg gcggaggctt tacacgcatc tcgtgttgat atctggaccg acgtcccggg
     3001 catctacacc accgatccac gcgtagtttc cgcagcaaaa cgcattgatg aaatcgcgtt
     3061 tgccgaagcg gcagagatgg caacttttgg tgcaaaagta ctgcatccgg caacgttgct
     3121 acccgcagta cgcagcgata tcccagtctt tgtcggctcc agcaaagacc cacgcgcagg
     3181 tggtacgctg gtgtgcaata aaactgaaaa tccgccgctg ttccgcgcgc tggcgcttcg
     3241 tcgcaatcag actctgctca ctttgcacag cctgaatatg ctgcattctc gcggtttcct
     3301 cgcggaagtt ttcggcatcc tcgcgcggca taatatttcg gtagacttaa tcaccacgtc
     3361 agaagtgagc gtggcattaa cccttgatac caccggttca acctccactg gcgatacgtt
     3421 gctgacgcaa tctctgctga tggagctttc cgcactgtgc cgggtggagg tggaagaagg
     3481 tctggcgctg gtcgcgttga ttggcaatga cctgtcaaaa gcctgcggcg ttggcaaaga
     3541 ggtattcggc gtactggaac cgttcaacat tcgcatgatt tgttacggcg catccagcca
     3601 taacctgtgc ttcctggtgc ccggcgaaga tgccgagcag gtggtgcaaa aactgcatag
     3661 taatttgttt gagtaaatac tgtatggcct ggaagctata tttcgggccg tattgatttt
     3721 cttgtcacta tgctcatcaa taaacgagcc tgtactctgt taaccagcgt ctttatcgga
     3781 gaataattgc ctttaatttt tttatctgca tctctaatta attatcgaaa gagataaata
     3841 gttaagagaa ggcaaaatga atattatcag ttctgctcgc aaacgaattc ccgtacactg
     3901 aactctctgg ctcactgagg gcaccatcat ggcactcccc cgtattaccc aaaaagagat
     3961 gactgaacgc gagcagcgtg aacttaaaac actgctggat cgggcgcgga ttgcgcatgg
     4021 tcgcgtactg accaactccg aaaccaacag cattaagaaa gagtacatcg ataaattaat
     4081 ggtcgagcgt gaggctgaag cgaaaaaagc ccgccagtta aagaaaaagc aggcttataa
     4141 accggatcct gaagcgtcat ttagctggtc agcaaatacg tcgacgcgcg gcaggcgctg
     4201 attagcgccc cttctttttt cgccccggcg aggtaaaacg cttaccgttg gacgccgggc
     4261 gaccgccttt ttccgccgtt ttttccatct taacgactgg acgtttgatg cccgctgttt
     4321 tcggtttggc cttcgcttta ggttttgcct cggaagagga attttcgatg agcttaaaga
     4381 ggtcgattaa ctcatcgtcg gttaaatcgc gccactcccc cagcggaatg ccgcttaagc
     4441 tgacgttcat aatgcgcgta cgttccagct ttttcacttc atagccgaaa tgctcgcaca
     4501 tgcgccggat ctgacggttc agcccctgca ccagggtaat gcggaagaca aacggcgctt
     4561 cttttttgac tttgcacttt ttagtcaccg tcccgaggat cggcaccccc gcccccatac
     4621 cacgaataaa ttcatcggta atcggtttat cgaccgtcac cagatactct ttctcatgat
     4681 cgttacccgc acggaggatc ttattcacca gatcgccgtg attggtgagg aaaatcagcc
     4741 cctgggagtc tttatccagg cggccaatcg ggaacacgcg tttgctgtgg ttaacgaaat
     4801 cgacaatgtt atcgcgctcg ccatcttcgg tggtgctgac aatgcctacg ggcttgttca
     4861 gggcgataag taccaaatct tcggcttccc gaggctcaat caactgaccg tttactttca
     4921 caacgtcgcc gggtttcacc tgatcgccaa tggtggctcg cttgccatta aggaacacat
     4981 tgccttgctc gatatagcga tccgcttcgc ggcgtgagca aattccgctt tcgctgatgt
     5041 atttatttaa acggactgat gagtcgggca gcataaattc tcctgtaaaa cgcggaatat
     5101 accgtacctc ttagcgggga aaaacagggt tatgcggcaa cgtcgaaatt aaatgtgatc
     5161 tgccacgcct tttatcacaa aagtgtctcc ggaagtacaa tgcaaatttg cgtggcaaaa
     5221 taaggataat caacatatta cgaatcgctg cacacctgtg ctgataacta ctgcgcagag
     5281 aaacaaatgt gcaagagaaa gcgctatttc tccccatgca aagagtgaaa aatcgctttg
     5341 aaaggccatc atgatggggt tatatcagat caagcggtga gaaagcgtat ttgcacaaat
     5401 gtgcagaacc aggaggcggc aatggaaaac agtgacgata tccgtttgat tgtgaagatt
     5461 gcccaactct attacgaaca ggatatgacg caggcgcaaa tcgcgcgcga actgggtatt
     5521 taccgcacca acatcagccg cttgcttaaa cgaggccgcg atcagggaat tgtcaccatc
     5581 gccatcaact atgactacaa cgaaaatctc tggctggagc agcaactgaa gcaaaagttt
     5641 ggcctgaaag acgttgtggt ggtgtcggga aatgatgagg atgaagagac tcaactggcg
     5701 atgatggggt tacacggcgc gcaactgctg gatcgcttgc tggagcctgg cgatattgtc
     5761 ggtttttcct ggggtcgcgc ggtgagcgca ctggttgaaa acttgccgca ggcggggcaa
     5821 tcgcggcagt taatctgcgt accaattatt ggcggaccgt ccggtaaact cgaaagccgc
     5881 tatcacgtaa acacattaac ctacagcgcg gcagcgaagc tgaaagggga atcgcatctc
     5941 gcggattttc cggctctact ggataaccca ttaattcgta atgggatcat gcagtctcag
     6001 cactttaaaa ccatctctgc ctactgggat aatctggatg tcgccctggt gggaattggc
     6061 tcaccggcca ttcgcgacgg cgctaactgg catgcgtttt atggtggtga agagagtgac
     6121 gacctgaatg cccgccaggt tgctggcgat atttgctcgc gcttttttga tattcacggc
     6181 gaaatggttg aaacgaatat gagcgaaaaa acactctcta tcgaaatgaa taaattaaag
     6241 caggcacgat attccattgg cattgccatg agtgaagaaa aatacagcgg aattattggt
     6301 gcactgcgtg gaaaatatat taattgtctg gtaacgaata gcagcacagc tgaactatta
     6361 ctgaaataaa aatcttcccg atgtttatcg taataaaaat tacggcgggt ccctattatc
     6421 taaaatcagg agtctgttat gcaaacgtgg ttaaatttgc aggataaaat cattatcgtc
     6481 accggcggcg catccggtat tggtctggcg attgtagagg aattattagc acaaggcgca
     6541 aacgtacaga tggtagatat tcacggtggc gatggacaat atgaaggtca taaaggctat
     6601 cagttttggc cgaccgatat ttccagcgcc aaagaggtaa atcatacggt agcggaaatt
     6661 atccagcgtt ttggtcgcat cgacggtctg gtcaataacg ccggggtcaa tttcccgcgt
     6721 ctgctggtcg atgagaaagc gcctgccggg cagtatgaac tcaacgaagc tgcattcgaa
     6781 aaaatggtca atatcaacca gaaaggcgtt tttctgatgt cgcaggcggt ggcgcgacag
     6841 atggtcaaac aacatgatgg cgtgattgtg aatgtttcct cagaaagtgg gctggaaggc
     6901 tcagaaggcc aaagctgtta cgccgctacc aaagccgcgc tcaatagctt cacgcgctcc
     6961 tggtcgaaag agctgggtaa acacggtatc cgtgtggtcg gtatcgcgcc ggggattctg
     7021 gaaaaaaccg ggctacgcac gccggaatat gaagaggcgc tggcgtggac gcgcaatatc
     7081 accgtcgagc agctgcgtga aggctatacc aaaaacgcca ttcctattgg gcgcgccgga
     7141 agattagcag aaatcgctga ttttgtttgt tatctgctgt ctgaacgcgc cagctatatc
     7201 accggagtaa ccactaacat tgcgggcggc aaaacgcgcg gctaaggagg ctttatggtc
     7261 aacgccattt tttgtgccca cggcaaatta gcttgcgcca tgctggaatc ggttcagatg
     7321 gtttacggca acgccaatgt tgaagccgtt gaatttgtgc ccggtgaaaa cgccggagac
     7381 atcgtagcaa agctggaaaa attagtaagc attcacaatc aaaatgagtg gctgattgcc
     7441 gtggatttac agtgcggcag cccgtggaat gcggcagcga tgcttgcgat gcgcaatcct
     7501 cgtctgcggg taatcagcgg actttctctg ccgctggcgc tcgaactggt tgataaccag
     7561 gacagcatga atgttgatga actgtgtgaa cacctgacgc aaatcgcaaa gcaaacctgc
     7621 gttgtctgga aacaactggc aacgacagag gaggatttct gatgaacatt acgctcgccc
     7681 gcattgatga ccgcctgatc cacggtcagg tcactaccgt ctggtcgaaa gtggctaacg
     7741 cccagcgcat tattatctgc aatgacgaag tttataacga tgaagttcgc cggacattat
     7801 tgcgtcaggc tgcgccaccg ggaatgaagg ttaacgtggt caatattgaa aaagccgtcg
     7861 cggtttatca caacccgcaa tatcaggacg aaaccgtttt ttatcttttc actcgcccac
     7921 aggatgcctt agcgatggtt cgccaggggg taaaaatagg caccctgaat attggcggca
     7981 tggcctggcg gcccggtaaa aaacagttaa ccaaagcggt ttcattagat gatgatgata
     8041 ttaacgcatt tcatgagtta aataatctag gcgtcatact ggatttacgc gtcgtagcgt
     8101 cagatccctc aatcaatatt atctacaaaa taaacgaaca gttaatcgca aattaaataa
     8161 tagcgcctgt gtacatatgc tttaacaggc agggatgacg acacttaaaa ggtgacaaat
     8221 catggaaata agtaccctac aaatcattgc tatatttctt ttttcctgta ttgccggaat
     8281 gggcagcgtg ctggatgaat ttcagactca tcgcccgtta attgcctgta cggtgattgg
     8341 tttaattctc ggtgatttaa aaaccggaat aatactcggt ggtacgctgg aattgatagc
     8401 tctcggctgg atgaacgtcg gcgcggcgca atctccggat tctgcactcg ccagcataat
     8461 ctccgccatt ctggttatcg ttggtcagca aagcatcgcc accggaatcg ccatcgcgtt
     8521 gcctgtggct gcggcaggcc aggtgctgac ggtgtttgcc cgtaccatca ccgtagtgtt
     8581 ccagcacgcg gcggataaag cagcggaaga agcacggttt cgcactctcg atattctgca
     8641 tgtctccgcg cttggcgtgc aggcgctgcg cgtcgccatt ccggcactga ttgtctcgct
     8701 gttcgtcagc gccgatatgg tgagcaatat gttaaacgcc attcccgaat ttgtgacccg
     8761 aggtctgcaa attgctggcg gttttatcgt ggtagtcggt tacgccatgg tgctacgtat
     8821 gatgggcgtg aaatatttga tgcctttctt tttcctcggt ttcctcgcag gtggctacct
     8881 cgatctcagt ctgctggcgt tcggtggcgt cggcgtgatc atggccctgg tctacatcca
     8941 gctaaatcca cagtggcgta aagctgaacc tcaactcctg gcccccgctt ccaccgccct
     9001 tgaccaactt gacgattaac ggagccatca tggaacagag aaaaattaca cgcggcgatc
     9061 tggtgagcat gtttctgcgc tccaacctgc aacaggcgtc ctttaacttt gaacgtattc
     9121 acgggctggg tttttgctac gacatgatcc ccgccatcaa gcgactttac ccagtaaaag
     9181 aggatcaggt tgcggcgctc aggcgacacc tggtgttctt taataccacg ccagccgtat
     9241 gtggcccggt catcggcgtc actgccgcca tggaagaggc gcgggccaac ggcgcggaaa
     9301 ttgatgacgg caccattaac ggcatcaaag tcggtctgat gggaccattg gcaggggttg
     9361 gcgatccact ggtctgggga acgctgcgcc cgattaccgc cgcgctcggc gcatcgctgg
     9421 cgctgtctgg caacattctc ggcccgctgc tgttcttctt tattttcaac gcggtgcgtc
     9481 tggcgatgaa gtggtatggc ctacagctcg gctttcgcaa aggggtgaat atcgtcagcg
     9541 atatgggcgg gaatgtgctg caaaaactca ccgaaggcgc gtcgattctc ggactgtttg
     9601 tgatgggcgt gctggtcacc aaatggacgt caatcaacgt accgttggtg gtttcacaaa
     9661 cgcatgccgc cgatggctcc accgtcacca tgaccgtgca gaacattctc gatcaacttt
     9721 gccctggttt gctggcgctc ggtctgacgc tactgatggt tcgtctgctc aacaaaaaaa
     9781 ttaacccggt atggctgatt ttcgccctgt ttggcttagg gattatcggc aatgcgctgg
     9841 gcttcctgtc ctgattcttc gccccggcac gactgccggg gccatcgttc aacatgaggt
     9901 ggtttatgaa aacaacagct ctgcgtcttt atggtaaacg tgatttacgc ctggaaacct
     9961 ttgaccttcc tgaaatgcag gaggatgaaa tcctcgcgac ggtggtcact gacagcctgt
    10021 gcctctcttc ctggaaagag gccaatctgg gtgaaaacca taaaaaagta cccgacgatg
    10081 tggcgaccaa cccaatcatc atcggccacg agttttgcgg cgatattctg gccgtgggta
    10141 aaaagtggca gcacaaattc cagccgggtc agcgttatgt gattcaggcc aacctgcaac
    10201 tccccgaccg cccggactgc cccggctact ccttcccgtg ggtaggcggc gaggccacgc
    10261 atgtggttat tcccaacgag gtcatggaac aagattgcct gctggcatac gacggcgaaa
    10321 cctattttga aggctcgctg gttgaaccgc tttcctgcgt gattggcgcg ttcaacgcca
    10381 actatcatct tcaggaaggt agttataacc acacgatggg gattcgcccg caagggcgca
    10441 tgctgatcct cggcggcacc ggaccaatgg gactgttggc gattgattat gcgctacatg
    10501 gacccgttaa cccgtcgctg ctcgtcatta ccgataccga caacgataaa ttgagttatg
    10561 cgcgcaagca ctatccatca gaaccgcaaa cactgattca ttatctcaat gccgccgatg
    10621 cagcatttga tacgctaatg gcgctgagtg gcggtcacgg cttcgatgat attttcgtct
    10681 ttgtgcctaa tgaaggactg gtgactctcg cctcttcctt gctggcgaca gatggttgcc
    10741 tgaatttctt cgccggaccg caggataaac atttcagcgc gccaattaat ttctacgatg
    10801 tgcattatgc atttacccac tacgtgggca cgtcaggcgg caataccgac gacatgcgcg
    10861 cagcggtcaa attgattgaa gagaaaaaag tgcaggccgc aaaagtggta acacatattc
    10921 ttgggctgaa tgccgcgggc gaaaccacgc ttgaattgcc tgccgtcggc ggcggcaaaa
    10981 agctggtgta taccgggaaa tacctgccgc tgacgtcact cacgcagatt caggatcaag
    11041 cactggcggc gattctggcg cgtcatcagg ggatctggtc cggtgaggcg gagcaatatc
    11101 tgctcactca tgcagaggca atttcccatg attaatcgcg ctacttaact ctgcatgtcg
    11161 ctggctggtc accgcggcaa cttcgcgacc tgatgtgcgg ctgattgctg aagcggccca
    11221 atgtgcacga gaaggataaa aaaacatcag cgccttaagg ggcgctcaaa ttggtggcaa
    11281 agtacacatt gtttatgccg gatgcggaat aaagccttat tcggcctaca taatctcgaa
    11341 aattcaatat attgcagtta tctggtaggc ctggtaagcg tagcgcatca ggcaatgctg
    11401 tgtttgtcat cagtctcagc gcccggtgca atacgtgaca tcgtgagcca tgtcagtaag
    11461 tggttaaatt cgcagtggta gcatgccttc cgtgatttct tactaaccgg agaagtaatg
    11521 gaactgcttt tattgagtaa ctcgacgctg ccgggtaaag cctggctgga acatgcactg
    11581 ccgctgattg ctgaacagtt gcagggtcgc cgctcagcgg tgtttatccc tttcgctggc
    11641 gtaacgcaga cctgggatga ttacacagcg aaaacggctg cggttctcgc tccgctgggt
    11701 gtttctgtca ccggtattca tagcgttgtc gatcccgttg ccgcgattga aaatgcggaa
    11761 attgtgattg tcggcggcgg taatactttc cagttgctga aacagtgccg cgagcgcggg
    11821 ctgctggctc caattactga cgtggttaaa cgtggcgcgc tgtatattgg ctggagcgca
    11881 ggcgctaacc ttgcttgccc aaccattcgt accaccaacg atatgccgat tgtcgatccg
    11941 caaggtttcg atgcgctaaa tctgttcccg ttgcaaatca acccgcactt caccaacgcg
    12001 ctgccggaag gccataaagg tgaaacccgt gagcagcgta ttcgcgaact gctggtcgtc
    12061 gcgccagaac tgacgattat tggtctaccg gaaggtaact ggatcacagt gagtaaaggt
    12121 cacgcaacgc tgggtggccc gaataccacc tatgtgttta aggccggtga agaagcggtt
    12181 ccgctggaag ctggtcaccg tttttaagtg aattgccgga tgcatgatcc ggcatcttgt
    12241 tcatagtgcc tgatgcgacg cttgacgcgt cttatcaggc ctacaggatt cacaatatta
    12301 atactcgtcc cgtccctcat cttcatcagg ctgttccagc acactgtaag ccaccgaaca
    12361 aaacagcgag ttcagacgct gcatatctcc cagtaagcct aaatgtagcg aactggtttc
    12421 aatgctttgc acgttttgct gatgcaggcg atcgacgtgg gcgtgggaat agcggcgatt
    12481 aagaatgcga aagcgatgct tgctgcggcg caaacgacga gcgctggtga catcgccaga
    12541 gaagaacacc gacattgcca gctttaaatt actgagcaat tgctcataga gcgcatccag
    12601 ttccttcaag ccatcaagcg aaaatgctcg ccttgcagcc agcgatttgt cggcaatttc
    12661 actactcatg cgctcgacga tatcggaggc ctgttcaagg ttgagcgaca tttcgatgat
    12721 ctccgcccag cggcgcgact cttcttctgc cagttcctct tttggcatcc gcgccagata
    12781 cagcttaatg gcggtataga gaacgttgat atcatctgcc agcttacgca gctctttctc
    12841 ctgccttggc tcgccgtgca tcactttatt cagcccttcc atcatctgtt ccatggcgtc
    12901 gccaatgcgc agggtttcgc gcgcggcgtt cgccagagca agcgtgggcg tatccagcgc
    12961 gctgacatcc agatgtttag gccgtagctg ggtatccagt tccggttcat cgcgaataat
    13021 cgttttgcaa aaccgtgcca tcgggtcaac aaatggcagc atggccaggc aacgtacaag
    13081 gttgtagaag acgtggaaat agatcaccag ttccgctttt ggcaatgaca acttccccat
    13141 tgtctctgcc agcaaatgga caaacggcag gataatcagg ctacccacca gcttaaacag
    13201 cagactaccc agcgcgacac ggcgggctgc ggcattggcg gcactgttgt tgagcatcgc
    13261 cagcaggccg gaaccgaggt tagcaccaat caccagacag agcgccacgg ggaaggagat
    13321 aatgcctgcg gcggtcagag tcgcggtcag cagtacagca gcaaggctgg agtagctgat
    13381 aatggcgaac atcgcgccaa tcagcgcatc cagcagaata tcgccggtca gcgaggcaaa
    13441 gatcacctga acgccgtttg cctgggtgat cggcgttacg gcctgcacaa tcaactccag
    13501 cgccagcaaa atcagcccaa gaccaatacc gacgcggccc agttgcccgg cgcgtgactg
    13561 tttgcgtccg aggaaaaaaa tcacgccgat aaaaataagt aacggtgaca gccaggataa
    13621 gtcgaaggtg agaatacgcg ccattagcgc cgtcccgaca tctgccccca gcacaatgac
    13681 cagagccggt gcgagggcta ccagatcctg agcgacgaac gaggtcacca gcatggtggt
    13741 ggcattactg ctctgtacca gtgcggtaac gccgatcccc gcgcaaaagg cgagcggctt
    13801 tttttcgacg ctccggctaa ggacagtacg caaacgcgcg ccgaagacgc gcattacgcc
    13861 ggttcgaaca atatgagtcc cccagaccag cagggcgacg gcagaaagca ggttaagcag
    13921 cgttaacacg agttcaggtt ctccttatcg ttatatgttc ctgaagcaat tgaatgacca
    13981 aaggaccgga aacacgctga ctggcatctt tactgtgcct tttgacattc gtttttccgg
    14041 ctaagattat tccagcctgt ttccagtata agtgtttcat attaataaag agacagggca
    14101 ctcattgagc gccctgtttg ttgtaaggaa aagtgacaga tttgtgaatc agtccgcgtc
    14161 ataccccaga ttcggtgcca gccagcgctc aacatcggag acgctcatac ctttacggcg
    14221 ggcatagtct tcaacctgat cgcgctgaat ttgcgccacc gcgtagtact tgctgtccgg
    14281 gtggctgaaa taccaacccg aaaccgatgc accgggccac atggcgaaag attctgtgag
    14341 tttcatgccg gtatgttttt ccacttccag cagctcccag atggtggctt tttccgtatg
    14401 ttccgggcag gccgggtagc ccggtgccgg acggatgccc tggtagtttt cgcggatcag
    14461 ttcttcgttg ctgaggttct cgttcggcgc atagccccag tagactttac gcacacgctc
    14521 atggagatac tccgcaaagg cttcggctaa acggtcggca agcgctttca ccatgatttt
    14581 gttgtaatca tcgtgctgcg cttcaaaggc atcagccagt gcgtcctctt ccagcccgcc
    14641 agtcacggca aatgcgccga tgtaatctgc tttaccagaa agcttcggcg caacgaagtc
    14701 agcgagacag tagttagcga atcccgtttt ttcggtctgt tgacgcagat ggtggctgac
    14761 gttgatcaca tgggtgcgcg tttcgtcacg gtagatttca atgtcatcgc ccacacggtt
    14821 tgccgggaac aggcccacca cgccacgcgg attcagcgtt ttctcggcgc ttaatttatc
    14881 cagcatgtcg ttggcgtctt taaacagccg ctgcgcctca acgcccacca cttcatcttc
    14941 cagaatgcgc ggatacttcc cggccagcga ccaggtcata aagaacggtg tccagtcgat
    15001 gtaattacgc agagtttcga tgctggcttc aacttcctgc acgccgagac ggtgcgccac
    15061 cggcggcgtg taagcctgcc agtcaaaagc gaagtcgtta tcgcgcgccg cttccagcgt
    15121 gaccggtggt gtgcgtggtt tcttgcgccc gtgctgaata cgtacggttt cgtactcctt
    15181 gcgggtacga gcgacaaaat catcgcgctg agtatcggaa agcagcgccg ccaccacacc
    15241 aacggtgcgc gaggcattct gcacatacac cgtcgggccg ctgtagttct gctcgatttt
    15301 caccgccgtg tgcgcttttg aggtcgtcgc gccaccgatc agtaacggaa tggtgaagcc
    15361 ctgacgctcc atctctttcg ccacgttaac catctcgtcc agcgacggcg tgataagccc
    15421 cgaaagtcca atcagatcag cattcacttc tttagcggta cggagaattt tttccgcagg
    15481 caccataacg ccgagatcga caatttcgta gttgttacat tgcagcacca caccaacgat
    15541 atttttaccg atgtcgtgga cgtcgccctt cacggtggcg atcaccatct tgccgttggt
    15601 tttgccctgc tctttgctgg cttcaataaa cggttcgagg taggccactg cctgcttcat
    15661 cacacgcgcc gatttgacca cctgtggcag gaacattttc ccttcgccga acagatcgcc
    15721 gaccacgttc atgccgtcca ttagcgggcc ttcaatcact tcaatcgggc gcgtagcctg
    15781 ctggcgggct tcttcggtat cctgctcgat aaactcagta atgcctttga ccagcgagta
    15841 ttccagacgt ttattcactt cccacgagcg ccactccgcc tgctgggcgt tagcggtgtc
    15901 gtcggttttg ctgccgcgat atttctcggc aagctccagt aaacgctcag tgccatcatc
    15961 gcgacgatta agaatcacat cttccaccgc gtcgcgcagt tcagcgggca ggtcgtcgta
    16021 aatcgccagt tgcccggcgt tgacgatccc catatccatg ccattgcgaa tagcgtagta
    16081 gaggaacacc gcgtgaatgg cttcgcgcac cggatcgttg ccacggaatg agaaagaaac
    16141 gttagatacg ccgccggaaa tcagcgcgtg cggcagttcg cgtttgatgt cttcgcaggc
    16201 gccgataaag tcctgcgcat agttgttgtg ctcttcaatg ccggtggcga ccgcgaagat
    16261 gttcgggtcg aagatgatat cttctggcgg gaagccaacc tcttcggtga ggattttgta
    16321 cgcccgacgg caaatctcaa ttttccgtgc gcgggtatcg gcctgcccct gttcgtcaaa
    16381 ggccattacc accaccgctg caccgtaacg acgcaacagt ttcgcatgat ggataaaggc
    16441 gtcgacgccc tctttcattg agatagagtt aacaatgcct ttgccctgaa tacacttcag
    16501 ccccttttcg atgacctccc attttgagga gtcgatcata atcggcacgc gagcgatatc
    16561 cggttcaccg gcaatcagat tgagaaaacg caccatcgcc gcttcggcat cgagcatccc
    16621 ttcatccatg ttgatatcga taatctgcgc gccgttttcc acctgctgac gcgctacatc
    16681 cagcgcctcg ctgtatttct cttctttgat caggcgcttg aacttagcgg aaccggtgac
    16741 gttggtgcgt tcacccacgt tcacaaacag gctatcgtcg ccaatgttca gcggctccag
    16801 gccggacaaa cggcaggcta cgggaatttc aggcagtttg cgcggcgcta atccttctac
    16861 tgcacgactc atcgctgcaa tatgttgtgg cgtggtgcca cagcagccgc cgacgatatt
    16921 gagaaaaccc gcttgcgccc attcacgtat ctgttttgcc atcgtgtcgg cgtcgagatc
    16981 gtactcacca aaggcgttgg gtagcccggc gttcgggtgc gcggtgacgt agcattccgc
    17041 aatccgtgat agttcctgca cgtactggcg cagttcatct ggccccagcg cgcagttgag
    17101 gccaaaagtc agggcttcgg catggcgcag ggagttgtaa aatgcttcgg tggtctgtcc
    17161 ggagagcgtg cgcccggagg cgtcggtgat ggtgccggag atcataatcg gcagctcaac
    17221 gcctaacgct tcgaactccg ttttcaccgc aaagaccgcc gctttggcgt taagggtgtc
    17281 gaaaacggtt tcaatcagaa tcagatccgc accgccttcc actaatgctt tggtggattc
    17341 gcgataggcc gccaccagct ggtcaaaagt gatgttgcgg aatgccggat cgttcacgtc
    17401 cggagaaatg gacgccgtgc ggttggttgg gccgagaacg ccggcaacgt agcgcggttt
    17461 ctccggcgtg cgcgcggtcc actcgtcagc acaggcgcgc gccagtttcg ccgccgcaaa
    17521 gttgatttcc gccgacaggg attccatctg gtaatccgcc atcgcaatgg tcgtggagtt
    17581 gaaggtgttg gtttcgatga tatccgcgcc cgcttcaaag taggcgttgt ggatagcggc
    17641 gatcacttcc ggtttgctta gcaccagcag atcgttattg cctttgaggt cgcatggcca
    17701 gtcggcaaag cgttcaccac gaaaatcggc ttcgttcagt cgatagctct ggatcatggt
    17761 gcccataccg ccgtccagca ccagaatacg ttcatttaac tgcgcacgca gttgttccac
    17821 tttgctgctc acacttgctc ccgacacgct caacccagat ttaataaaaa ttcaacaaac
    17881 catactggca taaacgcatc tgtggtaaaa gcgaccacca cgcaacatga gatttgttca
    17941 acattaactc atcggatcag ttcagcaact attgcattag ctaacaataa aaatgaaaat
    18001 gatttccacg atacagaaaa aggagactgt catggtcgca cccattcccg cgaaacgcgg
    18061 cagaaaaccc gccgttgcca ccgcaccagc gactggacag gttcagtctt taacgcgtgg
    18121 cctgaaatta ctggagtgga ttgccgaatc caatggcagt gtggcactca cggagctggc
    18181 gcaacaagcc gggttaccca attccacgac ccaccgcctg ctaaccacga tgcaacagca
    18241 gggtttcgtg cgtcaggttg gcgaactggg acactgggca atcggcgcac atgcctttat
    18301 ggtcggcagc agctttctcc agagccgtaa tttgttagca attgttcacc ctatcctgcg
    18361 caatctaatg gaagagtctg gcgaaacggt caatatggcg gtgcttgatc aaagcgatca
    18421 cgaagcgatt attatcgacc aggtacagtg tacgcatctg atgcgaatgt ccgcgcctat
    18481 cggcggtaaa ttgccgatgc acgcttccgg tgcgggtaaa gcctttttag cccaactgag
    18541 cgaagaacag gtgacgaagc tgctgcaccg caaagggtta catgcctata cccacgcaac
    18601 gctggtgtct cctgtgcatt taaaagaaga tctcgcccaa acgcgcaaac ggggttattc
    18661 atttgacgat gaggaacatg cgctggggct acgttgcctt gcagcgtgta ttttcgatga
    18721 gcaccgtgaa ccgtttgccg caatttctat ttccggaccg atttcacgta ttaccgatga
    18781 ccgcgtgacc gagtttggcg cgatggtgat taaagcggcg aaggaagtga cgctggcgta
    18841 cggtggaatg cgctgacttt ttctggcggg cagaggcaat attctgcccg tcatacctga
    18901 gtggtaatag aataagggtg tctgttaatc gcattgacgt caaaataact tattgtcata
    18961 cacttcatac acttttatta actcagcatt atttttaaac atcaaaccac ttaattatta
    19021 acaaaatcgc aagatctc
//
LOCUS       DBFIFM010000076        18897 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.076, whole genome shotgun sequence.
ACCESSION   DBFIFM010000076 DBFIFM010000000
VERSION     DBFIFM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.076"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(101..1468)
                     /gene="rhlE"
                     /locus_tag="N7C74_002335"
     CDS             complement(101..1468)
                     /gene="rhlE"
                     /locus_tag="N7C74_002335"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HGU2432196.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRK
                     PAAAQ"
     gene            1697..2368
                     /gene="cecR"
                     /locus_tag="N7C74_002336"
     CDS             1697..2368
                     /gene="cecR"
                     /locus_tag="N7C74_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HGU2432197.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            2371..3366
                     /gene="hlyD"
                     /locus_tag="N7C74_002337"
     CDS             2371..3366
                     /gene="hlyD"
                     /locus_tag="N7C74_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HGU2432198.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            3359..5095
                     /gene="ybhF"
                     /locus_tag="N7C74_002338"
     CDS             3359..5095
                     /gene="ybhF"
                     /locus_tag="N7C74_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGU2432199.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            5088..6221
                     /gene="ybhS"
                     /locus_tag="N7C74_002339"
     CDS             5088..6221
                     /gene="ybhS"
                     /locus_tag="N7C74_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2432200.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            6232..7338
                     /gene="ybhR"
                     /locus_tag="N7C74_002340"
     CDS             6232..7338
                     /gene="ybhR"
                     /locus_tag="N7C74_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2432201.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(7300..7710)
                     /gene="ybhQ"
                     /locus_tag="N7C74_002341"
     CDS             complement(7300..7710)
                     /gene="ybhQ"
                     /locus_tag="N7C74_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HGU2432202.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            7843..8604
                     /gene="ybhP"
                     /locus_tag="N7C74_002342"
     CDS             7843..8604
                     /gene="ybhP"
                     /locus_tag="N7C74_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGU2432203.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNTSASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            8601..9842
                     /gene="clsB"
                     /locus_tag="N7C74_002343"
     CDS             8601..9842
                     /gene="clsB"
                     /locus_tag="N7C74_002343"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HGU2432204.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            9842..10798
                     /gene="ybhN"
                     /locus_tag="N7C74_002344"
     CDS             9842..10798
                     /gene="ybhN"
                     /locus_tag="N7C74_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HGU2432205.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHVPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(10834..11547)
                     /gene="ybhM"
                     /locus_tag="N7C74_002345"
     CDS             complement(10834..11547)
                     /gene="ybhM"
                     /locus_tag="N7C74_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HGU2432206.1"
                     /translation="MESYLQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLIKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIVVFSTAGAMFLISMLAGLLFNIDPGSHRFMIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWSGIILHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(11755..12459)
                     /gene="ybhL"
                     /locus_tag="N7C74_002346"
     CDS             complement(11755..12459)
                     /gene="ybhL"
                     /locus_tag="N7C74_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HGU2432207.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDASNLRKYSILCALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(12595..13047)
                     /gene="moaE"
                     /locus_tag="N7C74_002347"
     CDS             complement(12595..13047)
                     /gene="moaE"
                     /locus_tag="N7C74_002347"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HGU2432208.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFIGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(13049..13294)
                     /gene="moaD"
                     /locus_tag="N7C74_002348"
     CDS             complement(13049..13294)
                     /gene="moaD"
                     /locus_tag="N7C74_002348"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HGU2432209.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVSGG"
     gene            complement(13287..13772)
                     /gene="moaC"
                     /locus_tag="N7C74_002349"
     CDS             complement(13287..13772)
                     /gene="moaC"
                     /locus_tag="N7C74_002349"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HGU2432210.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(13775..14287)
                     /gene="moaB"
                     /locus_tag="N7C74_002350"
     CDS             complement(13775..14287)
                     /gene="moaB"
                     /locus_tag="N7C74_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HGU2432211.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(14309..15298)
                     /gene="moaA"
                     /locus_tag="N7C74_002351"
     CDS             complement(14309..15298)
                     /gene="moaA"
                     /locus_tag="N7C74_002351"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HGU2432212.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(15286..15430)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            15695..16603
                     /gene="yvcK"
                     /locus_tag="N7C74_002352"
     CDS             15695..16603
                     /gene="yvcK"
                     /locus_tag="N7C74_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HGU2432213.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(16794..18815)
                     /gene="uvrB"
                     /locus_tag="N7C74_002353"
     CDS             complement(16794..18815)
                     /gene="uvrB"
                     /locus_tag="N7C74_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HGU2432214.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT         4434 a         5027 c         4966 g         4470 t
ORIGIN      
        1 aatttattaa aacaataaaa tcagacatgt catgcatgtc tgaccgtgtt acctgaataa
       61 tgggcaaaag cctgtgatac tcttttgccc ttacatggta ttactgcgca gcggcaggtt
      121 tacgcggacg gcgacggcgt tgttgttcgc ctgccggttt ggcatcgcca agacgacgag
      181 atggtttttc tgcaggcttc gcgctagctg atttcgcacc accttctcca cggcgtggtt
      241 gctgttgacc gcgaccacca ccaccttgcc cacgaccgcc gccgccacgt tgctggcgac
      301 cgttctggat cggttcggct ttgattgacg ggtccggctc ataacccgga atcgcaatgc
      361 gcgggatctc ttttttcagc agtttttcga tatcacgcag cagtttgtgt tcatcaacac
      421 acaccagcga caatgcttcg ccagttgccg ccgcacggcc agtacgcccg atacggtgga
      481 cataatcttc cggtacgttt ggcagttcat agttgacgac gtgcggcaac tcttcgatat
      541 ccagaccgcg cgcagcgatg tcagttgcca ccagtacacg aatatcgccc gatttaaaat
      601 cggccagcgc acgggtacgc gcaccttgcg atttattacc gtggatcgcc gcactacgga
      661 tgccatcttt attaagctgt tcagcgagat ggttagcgcc atgtttggta cgggtaaaca
      721 ccagcacctg ctgccagttc cctttcccaa tcatgtgcga cagcaattcg cgcttgcgtt
      781 tcttatcaac aaagtgaaca tgctgggtca cctgatcaga cgcggtattg cggcgtgcca
      841 cttctatttc cagcgggttg tgcaacagtt tttccgccag ggctttaata tcgtcagaga
      901 aggtcgcgga gaacaacagg ttctggcgct tcgcaggtag ttttgttaac acgcgacgga
      961 tatcgtggat aaagcccatg tcgagcatgc ggtcagcttc atcgaggacg aggatttcaa
     1021 cctgatccag cttcactgca ttctgatgtt ccaggtccag caaacgtccc ggcgttgcca
     1081 ccagcacatc aacgccgccg cgcaacttca tcatttgtgg gttaatgctg acaccgccaa
     1141 acaccaccag cgaacgaatg ttcaggtatt tgctgtaatc acggacgttt tcgccaatct
     1201 gcgccgccag ttcacgggtc ggggtaagaa tgagcgcacg taccggacga cgccctttgg
     1261 cgtgcggctg gcgagtgatc aggtgttgca acagcggcag cgtaaagccc gctgttttgc
     1321 cggtgccggt ctgggcgcta gccatcaggt cgcggccttc cagcaccgca gggatcgcct
     1381 gctgctgaat aggggtgggt tcacggtaac cctgctcggc aacggcgcgc aggatatcag
     1441 ggcttaaacc caaagaatcg aaagacataa ctactccgaa ccgccccgac cgttaccggt
     1501 gtagttttca gggagatact gaaaaggaaa atgacaaaaa ccacaatgtc atgagggagc
     1561 ggagtgtagc agtttttgtg acgcagcgca taaattatcc ctgtgactgg cgtagctaaa
     1621 attcttagcc atactggaca actcccatat ggagtcataa tcttaatcaa tcatttgatt
     1681 aagaatggac tgtgcgatga ataatcctgc catgacaatc aagggtgaac aggcgaaaaa
     1741 acagctgatt gctgccgcac tggcgcagtt tggtgaatat ggaatgaacg ccaccacccg
     1801 cgagatagcc gcccaggccg ggcagaatat cgctgccatc acctactact tcggttcgaa
     1861 agaagatttg tacctcgcct gcgcccagtg gattgccgat tttattggcg agcagttccg
     1921 tccgcatgcc gaggaagccg aacgcttgtt cgcccaacca cagcctgatc gggctgccat
     1981 ccgtgaactg atccttcgcg cctgcaggaa catgattaag ctgctcaccc aggatgacac
     2041 cgtcaacctc agcaagttta tctcccgtga gcagctctct cccacggcag cctaccacct
     2101 ggtgcacgaa caggtgatta gtccgctaca cagccacctg acacgcctga ttgccgcctg
     2161 gaccggctgc gacgccaatg acacccgcat gatccttcat acccatgcgt tgattggtga
     2221 gattctggcg ttccgtcttg gcaaagaaac aattctgtta cgtaccggct ggaccgcgtt
     2281 cgatgaagaa aagaccgaac tgatcaacca gacggtgacc tgtcatatcg acctgatttt
     2341 gcaaggatta tcgcaaagga gtttgtagtg atgaaaaaac ctgtcgtgat cggactggcg
     2401 gtagtggtac ttgccgccgt ggttgccgga ggctactggt ggtatcaaag ccgccaggat
     2461 aacggcctga cgctgtatgg caacgtggat attcgtacgg tgaatcttag tttccgtgtt
     2521 ggggggcgcg ttgaatcgct ggcggtggac gaaggtgatg ctatcaaagc gggccaggtg
     2581 ctgggcgaac tggatcacaa gccgtatgag attgccctga tgcaggcgaa agcgggcgtc
     2641 tcggtggcac aggcgcagta tgacctgatg cttgccgggt atcgcgatga agaaatcgct
     2701 caggccgccg cagcggtgaa acaggcgcaa gccgcctatg actatgcgca gaacttctat
     2761 aaccgccagc aaggactgtg gaaaagccgc accatttcgg caaatgacct ggaaaatgcc
     2821 cgctcctcgc gcgaccaggc acaggcaacg ctgaaatcag cacaggataa attgcgtcag
     2881 taccgttccg gtaaccgtga acaggacatc gctcaggcga aagccagcct cgaacaggcg
     2941 caggcgcaac tggcgcaggc ggagctgaat ttacaggact caacgttgat agcaccgtct
     3001 gatggcacgc tgttaacgcg cgcggtggag ccaggcacgg ttctcaatga aggtggcacg
     3061 gtgtttaccg tttcactaac gcgtccggtg tgggtgcgtg cttatgttga tgaacggaat
     3121 cttgaccagg cccagccggg gcgcaaagtg ctgctttata ccgatggtcg cccggacaag
     3181 ccctatcacg ggcagattgg tttcgtttcg ccgactgctg aatttacccc gaaaaccgtc
     3241 gaaacaccgg atctgcgtac cgacctcgtc tatcgcctgc gtattgtggt gaccgacgcc
     3301 gatgatgcgt tacgccaggg aatgccagtg acggtacaat tcggtgacga ggcaggacat
     3361 gaatgatgcc gttatcacgc tgaacggcct ggaaaaacgc tttccgggca tggacaagcc
     3421 cgccgtcgcg ccgctcgatt gcaccattca tgccggttat gtgacggggc tggtagggcc
     3481 agacggtgcg ggtaaaacca cgctgatgcg gatgctggcg ggattactga aacccgacag
     3541 cggcaatgcc acggtgattg gctttgatcc gatcaaaaac gacggcgcgc tgcacgccgt
     3601 gctcggctat atgccgcaga aattcggcct gtatgaagat ctgacggtga tggagaacct
     3661 caatctgtac gcggatttgc gcagcgtcac cggcgaggca cggaagcaga cttttgctcg
     3721 cctgctggag tttacgtctc ttggcccgtt taccggacgc ctggcgggca agctctccgg
     3781 tgggatgaaa caaaaactcg gcctggcctg taccctggtg ggcgaaccga aagtgttgct
     3841 gctcgatgaa cccggcgtcg gcgttgaccc tatctcacgg cgcgaactgt ggcagatggt
     3901 gcatgagctg gcgggcgaag ggatgttaat cctctggagt acctcgtatc tcgacgaagc
     3961 cgagcagtgc cgtgacgtgt tactgatgaa cgaaggcgag ttgctgtatc agggagaacc
     4021 aaaagccctg acacaaacca tggccggacg cagctttctg atgaccagtc cacacgaggg
     4081 caaccgcaaa ctgttgcaac gcgccttgaa actgccgcag gtcagcgacg gcatgattca
     4141 ggggaaatcg gtacgtctga tcctcaaaaa agaggccaca ccagacgata ttcgccatgc
     4201 cgacgggatg ccggaaatca acatcaacga aactacgccg cgttttgaag atgcgtttat
     4261 tgatttgctg ggcggtgccg gaacctcaga atcgccgctg ggtgcaatat tgcatacggt
     4321 agaaggcaca cccggcgaga cggtgatcga agcgaaagaa ctgaccaaga aatttggtga
     4381 ttttgccgcc accgatcacg tcaactttgc cgttaaacgc ggggagattt ttggtttgct
     4441 ggggccaaac ggcgcgggca aatcgaccac ctttaagatg atgtgcggtt tgctggtgcc
     4501 gacttccggc caggcgctgg tgctggggat ggatctgaaa gagagttccg gtaaagcgcg
     4561 ccagcatctc ggctatatgg cgcaaaaatt ttcgctctac ggcaacctga cggtcgaaca
     4621 gaatttacgc tttttctctg gtgtgtatgg cttacgcggt cgggcgcaga acgaaaaaat
     4681 ctcccgtatg agcgaggcat ttggcctgaa aagtattgcc tcacacgcca ccgatgaact
     4741 gccattaggt tttaaacagc ggctggcgct ggcctgttcg ctgatgcatg aaccggatat
     4801 tctgtttctc gacgaaccga cttccggcgt tgaccccctc acccgccgtg aattctggct
     4861 gcacatcaac agcatggtag agaaaggcgt cacggtgatg gtcaccaccc actttatgga
     4921 tgaagcggaa tattgcgacc gcatcggcct ggtgtaccgc gggaaattaa tcgccagcgg
     4981 cacgccggac gatttgaaag cacagtcggc taacgatgag caacccgatc ccaccatgga
     5041 gcaagccttt attcagttga tccacgactg ggataaggag catagcaatg agtaacccga
     5101 tcctttcctg gcgtcgcgtg cgggcgctgt gcgtcaaaga gacgcggcag atcgttcgcg
     5161 atccgagtag ctggctgatt gcggtagtga tcccgctgtt gctgctgttt atttttggtt
     5221 atggcattaa cctcgactcc agcaagctgc gggtcgggat tttactggaa cagcgtagcg
     5281 aagcagcgct ggatttcacc cacaccatga ccggttcgcc ctacatcgac gccaccatca
     5341 gcgataaccg tcaggaactg atcgccaaaa tgcaggcggg gaaaattcgc ggtctggtgg
     5401 ttattcccgt ggatttcgcc gaacagatgg agcgcgccaa cgctaccgca ccgattcagg
     5461 tgatcaccga cggcagtgag ccgaataccg ctaactttgt gcagggatat gtcgaaggga
     5521 tctggcagat ctggcaaatg cagcgggcgg aggataacgg gcagaccttt gagccgctta
     5581 ttgatgtaca aacccgctac tggtttaacc cggcggcgat tagccagcac tttattatcc
     5641 ccggtgcggt gaccatcatc atgacggtca tcggcgcgat tctcacctcg ctggtggtgg
     5701 cgcgagaatg ggaacgcggc actatggagg ctctgctctc tacggagatt acccgcacgg
     5761 aactgctgct gtgtaagctg atcccttatt actttctcgg gatgctggcg atgttgctgt
     5821 gtatgctggt gtcagtgttt attctcggcg tgccgtatcg cggttcgctg ctgattctgt
     5881 tttttatctc cagcctgttt ttactcagta ccctggggat ggggctgctg atttccacga
     5941 ttacccgcaa ccagttcaat gccgctcagg tcgccctgaa cgccgcgttc ctgccgtcga
     6001 ttatgctttc cggctttatt tttcagatcg acagtatgcc cgcggtgatc cgcgcggtga
     6061 cgtacattat tcccgcacgt tatttcgtca gcaccctgca aagcctgttc ctcgccggga
     6121 atattccagt ggtgctggtg gtgaacgtac tgtttttgat cgcttcggcg gtgatgttta
     6181 tcggcctgac gtggctgaaa accaaacgtc ggctggatta gggagaagag catgtttcat
     6241 cgcttatgga cgttaatccg caaagagttg cagtcgttgc tgcgcgaacc acaaacccgc
     6301 gcgattctga ttttaccggt gctgattcag gtgattctgt tcccgttcgc cgccacgctg
     6361 gaagtgacca acgccaccat cgccatctac gatgaagata acggcgagca ttcggtggag
     6421 ctgacccaac gttttgcccg cgccagcgcc tttacacatg tgctgctgct gaaaagccca
     6481 caggagatcc gcccgaccat cgacacacaa aaggcgttac tactggtgcg tttcccggct
     6541 gacttctcgc gcaaactgga taccttccag accgcgccgt tgcagttgat cctcgacggg
     6601 cgtaactcca acagtgcgca aattgccgcc aactacctgc aacagatcgt caaaaattat
     6661 cagcaggagc tgctggaagg aaaaccgaaa cctaacaaca gcgagctggt ggtacgcaac
     6721 tggtataacc cgaatctcga ctacaaatgg tttgtggtgc cgtcgctgat cgccatgatc
     6781 accactattg gcgtaatgat cgtcacttca ctttccgtcg cccgcgaacg tgaacaaggt
     6841 acgctcgatc agctactggt ttcgccgctc accacctggc agatcttcat cggcaaagcc
     6901 gtaccggcgt taattgtcgc caccttccag gccaccattg tgctggcgat tggtatctgg
     6961 gcgtatcaaa tccccttcgc cggatcgctg gcgctgttct actttacgat ggtgatttat
     7021 ggtttatcgc tggtgggatt cggtctgttg atttcatcac tctgttcaac acaacagcag
     7081 gcgtttatcg gcgtgtttgt ctttatgatg cccgccattc tcctttccgg ttacgtttcg
     7141 ccggtggaaa acatgccagt atggctgcaa aacctgacgt ggattaatcc tattcgccac
     7201 tttacggaca ttaccaagca gatttatttg aaggatgcga gtctggatat tgtgtggaat
     7261 agtttgtggc cgctactggt gataacggcc acgacagggt cagcggcgta cgcgatgttt
     7321 agacgtaagg tgatgtaact tcttatcttt cgccagcaaa gacactaccg ccgggccggc
     7381 aaggattgcc agccctgcaa ttgccagcaa aaagttgttt tccagtacgc gggacaacag
     7441 ccacagcaca atcagcgctg cgccaaaata ccaggtcgtg gtcagctctt ccagcacgtc
     7501 tgccacctca cgccagcgtt gctcggggtg gcgctgaaac accagcatca tcactaccgt
     7561 gacacaatac agtgcgcata atcccacgcc gacgtgaacc agagtcttac tcatccagcc
     7621 caccaccagc agcgccacta ccagtaaatg caacataatc tgccagcaac ttagacccgt
     7681 tgcgacacga acacgttgtt gccacttcat ggcttctctc ctgaagaatt tatctctact
     7741 tactcagagt accgaacttt acggaaaatt ccgtaagacc gcaccttttt gtgacgccga
     7801 ctacactatt tcaatgagat aagaggcaaa gacaggaaaa ttatgcccga tcaaacacaa
     7861 caattttcgt tcaaggtgct caccatcaat attcacaaag gctttaccgc gtttaaccga
     7921 cgcttcattt tgccggaact tcgcgatgcc gtgcgtaccg tcagcgccga tattgtttgc
     7981 ctgcaggaag tgatgggcgc gcacgaagtt catccgctgc atgtggaaaa ctggcccgat
     8041 acctcgcact acgagtttct cgccgacact atgtggagcg attttgccta cggtcgcaat
     8101 gccgtatacc cggaagggca tcacggcaac gccgtactgt cgcgttatcc cattgaacat
     8161 tatgagaatc gcgatgtttc ggtcgatggt gcggaaaagc gcggcgtgct ctactgccgc
     8221 attgtgccgc cgatgaccgg aaaagcgatt catgtgatgt gcgtacatct gggcctgcgt
     8281 gaggcgcacc gtcaggcgca gcttgcgatg ctcgccgaat gggtgaatga gctaccggac
     8341 ggcgaaccgg tattggtggc gggtgatttc aatgactggc ggcaaaaagc taatcatccg
     8401 ttaaaagtgc aggccggact ggatgagatt tttacccgcg cccacggacg cccggcgcgc
     8461 acgtttccgg tgcaatttcc tctactacga ctggacagga tctacgtcaa aaataccagc
     8521 gccagcgcgc caaccgcgtt gccgctgcgg acatggcgac acctttctga tcatgcccct
     8581 ttaagtgcgg agattcattt atgaaatgta gctggcgcga aggcaataag atccagttgc
     8641 tggaaaacgg cgagcaatat tatcccgcgg tgtttaaggc gattggcgag gcacaagaac
     8701 gcatcattct tgaaacgttt atctggtttg aggatgacgt cggcaaacaa ctgcatgcgg
     8761 cactactggc agcagcgcaa cgcggggtta aagcggaagt cttgctggat ggctacggtt
     8821 cgccggatct cagcgatgag tttgtcaatg aactgacggc agctggcgta gtgttccgct
     8881 actacgatcc ccgccctcgc ctttttggta tgcgcaccaa tgtgtttcgc cggatgcatc
     8941 gcaaaattgt ggtaatcgac gcgcgtatcg cctttattgg cgggctgaat tactccgccg
     9001 agcatatgtc cagctacggt ccagaggcta aacaggatta cgcggtacgc cttgaagggc
     9061 cgattgtcga agatattctc cagtttgagc tggaaaacct gcctggacag agcgcggcac
     9121 gacgctggtg gcgacgtcat cacaaagcgg aagagaaccg ccagccggga gaagcgcagg
     9181 tattgctggt ctggcgcgat aacgaagaac atcgcgatga tattgaacgt cactatctga
     9241 aaatgctcac tcaggcgcgg cgagaagtca ttatcgccaa cgcctacttc ttccccggct
     9301 atcgattttt acacgccttg cgtaaagcgg cacggcgcgg ggtgcggatc aaactgatca
     9361 ttcagggcga accggatatg ccgattgtca gagtcggtgc gcgcttgctg tataactatc
     9421 tggttaaagg cggcgttcag gtgtttgagt accgccgccg cccgctccac ggcaaagtgg
     9481 cattgatgga cgatcactgg gcgacagtag ggtccagtaa tctcgatccg ctcagtttgt
     9541 cactgaatct cgaagcaaat gtcatcatcc acgatcgtca ttttaaccag acgctgcgcg
     9601 ataatctgaa cggcattatt gccgcagatt gtcagcaggt cgatgagacc atgctgccga
     9661 aacgcacctg gtggaaccta accaaaagcg tgctggcgtt ccacttttta cgccacttcc
     9721 cggcgctggt tggctggctt ccggcacaca cgccacgtct ggcgcaggtt gatcctcccg
     9781 cacaaccgac aatggaaacg caggatcggg tagaaactga aaacacgggg gtaaaaccct
     9841 gatgagtaaa tcacacccgc gctggcgctt agcaaagaag atcctcacct ggctgttttt
     9901 tatcgcggtg attgtgttgc tggtggtcta cgccaaaaaa gtggactggg aagaggtctg
     9961 gaaggtcatc cgcgactaca atcgcgttgc gctgcttagt gcggtcgggc tggtggtcgt
    10021 cagctatctg atttacggct gctatgacct gctcgcccgc ttttactgcg gtcacaaact
    10081 ggcgaagcgc caggtgatgc tggtgtcgtt tatctgctac gccttcaacc tgacgctcag
    10141 tacctgggtc ggcggcattg gtatgcgcta tcgtttgtac tcccgtctgg gattgccggg
    10201 cagcactatt acgcggattt tctcgctcag tattaccacc aactggctgg gctatatttt
    10261 actggcaggg attatcttta ccgcaggcgt ggtggagttg ccggaccact ggtatgtcga
    10321 tcaaactacg ctgcgtattc tcggcattgg cttactgatg attatcgcgg tttatttgtg
    10381 gttttgcgct ttcgcgaagc accgccatat gaccatcaaa gggcaaaagc tggtgctgcc
    10441 ttcgtggaaa ttcgccctcg cccagatgct gatttccagc gttaactgga tggtcatggg
    10501 ggcgattatc tggctgttac ttggtcaaag cgtgaactat ttctttgtac tgggcgtgtt
    10561 gctggttagc agtattgccg gcgtcatcgt gcatgttccg gcggggattg gtgtgctgga
    10621 agcggtgttt atcgcgctac tggctgggga gcatacatcc aaaggcacaa ttatcgccgc
    10681 cctactcgct taccgtgtgc tgtattactt tatcccgctg ctgctggcgc tgatctgtta
    10741 cctgttgctg gaaagccagg cgaagaagct acgggcgaaa aatgaagcgg cgatgtgaag
    10801 tttagttaag ttctttagta tgtgcattta cgactaatga aaaaaacgcg tatgcctttg
    10861 ccagacaagc gttatagcta tcgcggcaag tatcccaaag aagccgataa aatagtaata
    10921 gagtgttaat gcacctgtaa aagcgcaacg aaccggactc cacaactctt cactatggca
    10981 tttccccgcc aattcaagga gcttatttcg tccatgcaag ataatgcctg accataacac
    11041 aatcattaag caacttatta cccaaatggg ccgttcactc attaatgcgg cattcacgat
    11101 gattaccagg gccaaccctg tcaacgtcat cataatcata aaacggtgag aaccaggatc
    11161 aatattaaat aataaaccgg ccagcatact gattaaaaac atcgcgcccg cagtgctgaa
    11221 cacgacgata atggcatata caggaactaa acttgcaacg acaatcgcca ccaaagagag
    11281 taaaaacaga tatattacgc ctgcgatggc ggcatttatc ttgttgatat ccttaattaa
    11341 gccatacata caaagtaata ataaccctat ggcgacatac attggtggtg aatcatattt
    11401 ggttgccgta atttcgagat acttagcccc ccaggccagc cctgcggttg ccaccaaacc
    11461 taaagcggta atgagccata taccgtttaa tatcctggtc tcgtgctgaa aatctaattt
    11521 attgctgttt tgcaagtatg attccattct tttagttgtc cttatagaaa aatattaata
    11581 ttcatcatga ataaatttaa acccacgcat acaaaatact tttgtattta tggatacgga
    11641 aaactcttcc gctcggccat aaggcgtatt ttatacctgg tacttgttaa gtgtcgtttt
    11701 tagtaagaaa aaaaatcccg acacatggcc gggatattga tggtgaaaaa agaattaacg
    11761 acgattgcca aaaattcgca gcaacatcag gaacaggttg atgaagtcca gatacaaggt
    11821 taatgcgcaa agaatggaat atttgcgcag gttcgacgcg tcgcgggtat ctatctgctc
    11881 acccatattt ttcagtttct gggtgtcata cgccgtcaat ccgacaaaga caatcacgcc
    11941 gatgtaggtg actgcccaca tcaacgcttc gcttttcagc cagaagttga ccagcgatgc
    12001 caacacaatg ccgattaacg ccataaacag catattgcca aagccactta aatcgcgctt
    12061 cgtggtgtaa ccgtacaggc tcattgcgcc gaacatcccg gcagtaacga cgaaggtact
    12121 ggcgatagaa gcagcggtat aaacaatgaa aatactggaa agcgtaagac ccgtcagcgc
    12181 cgaataaagc ataaagagca tcgtcgttac acctgcgctc agcttttgaa tcatcgctga
    12241 taacacaata accaatgcta attgcgcgat gatcagaccg attaaaaaga cacggttagt
    12301 gaacaacagc tccatcacgg ccgcggaatt agccgcatac caggcaacaa atgcggtcag
    12361 caataagcca acggtcatcc agccatagac ttgcgccata taagtctgca agccagcccg
    12421 gggttgtaca attgaatcag aacgtgggaa tctgtccatg acgatctcct gaagatataa
    12481 ggaatatctt aaggatactg caaaatgatg aggctgtgca tcgacgcagc gtaaacgcat
    12541 gtactgagcg gtgaaattgc cggacgcagc ggtgccttat ccggctaaca aaaactacca
    12601 gcgttttgcc gcctgctgat cgctctcccg agcttcgacc cagcggtcgc cctccggcgt
    12661 ggcttcgcgc ttccagaacg gtgcgcgggt tttgagataa tccataataa actgcccggc
    12721 ttcaaacgca ctgctgcgat gcgcactggt gacaccgata aaaacgattt catcgcccgg
    12781 ccataattcc ccgatgcggt gaatcaccgt gacgcgcccc agcggccagc ggttacgcgc
    12841 ttcatcgaca atttctgcca gtgctttttc agtcatcccc ggatagtgtt cgagggttaa
    12901 tgctttgacg ctgtcgccca ggttatggtt gcgcacctta ccggtaaagg tgactaccgc
    12961 accgtcttcg tcacgctccg ccagccacgg gtactcttct cctacgctga acggctgcgg
    13021 accaacaaca attttggttt ctgccatctt aacctccgct taccggcggg aagaaagcta
    13081 cttcgtcgcc gtcagtcagc ggatggtcaa aactcaccag cgtctggttg acggcagcca
    13141 gtaatttgcc atcttccagc gccagcgccc agcgatcgct ctgcgcagcc aggtgctggc
    13201 gtaacgcttc aacggttggg aaatccgcag ccacttcggt tgcatctgtt cctaccaact
    13261 cgcgcacctg ggcgaaaaaa agaactttaa tcatccgctt ccaccttaaa gtcacccgac
    13321 ttgccgccgc tcttcgccag caaacgtacc ggaccaatca ccatatcttt ttgcaccgct
    13381 ttgcacatgt cataaatggt cagcgccgcc acggaggccg cggttaacgc ttccatttcg
    13441 acaccggttt tcccggtcag gcggcataag gtttctatac gcacccgatt gtgctccggc
    13501 tcggcctgta aattgacttc aactttgctg agcatcagcg gatgacacag cgggatcaga
    13561 tcccaggtgc gttttgccgc ctgaataccg gcaatacgcg cagtggcaaa tacgtcgcct
    13621 ttgtggtggc gaccatcaat aatcatcgcc agcgtctcgc tgcgcatggt gacaaaggct
    13681 tccgcccgcg cttcacgcac ggtttccgct ttggcggaga catccaccat gtgcgcttcg
    13741 ccagcggcgt tgatatgggt cagttgcgac atacttattt cttcaaatgt ggatggaaat
    13801 tacacggacg cgtacgggca tccagctgcg gcgcgatgat attttcccat gcggtacggc
    13861 acgctttggt cgaacccggc atggcgaaaa tcagcgtttt gttagcgacg cccgctaccg
    13921 cacgagattg caacgtggaa gtgccaatct cttcaaacga caacatacgg aacacttcac
    13981 caaaaccttc aacttcacgg tcgaacaacg gcagcaatgc ttcgggagcc tgatcacctt
    14041 ccgtcaggcc agtccccccc gtaatcaaca ccacttgtac atcgtcgctg gcgatccacg
    14101 cagatacctg agcgcgaata gcgtagcggt tttctttcac aatggcttta tcgacaacgt
    14161 gatggcccgc ttcttgcgcc gaatcgcgca aatagtgacc ggaggtatcg tcttcttcac
    14221 cgcgacgatt agaaaccgta agaatagcaa tacgggtcgg gataaattca gtgcttacct
    14281 gactcatctg atctctcctt ttgacgtttt agccgccaat gtacgataag ttttgcgtaa
    14341 taccggtgtt gttttgatgc aggaaatggg tctgtttctt ctcccgcagc gccgctgaaa
    14401 tacgcgcttc cagcgcctgt tgctgggtat cgtcttccag cagatcgcgc aggttaacgc
    14461 cgccttcacc aaacaggcag agatggagtt taccaatgga ggaaacgcgc agacggttgc
    14521 aagtggcgca gaagtctttt tcatacggca tgataaggcc aatctctccg gcgtaatccg
    14581 gatgacaaaa gacttgcgcg ggaccgtcgc tgcgttgacg taattggtgg atccagccgc
    14641 gacgcagtag ctcgtcacgc agaacctgac cagagatgtg atgcttacgg aaaagctcgc
    14701 tgccctcgcc cgtttccatc agttcgatga aacgcagctg gatagggcga tgctggatcc
    14761 agttcagaaa ggtgtcgagc tggtgatgat tcacatcacg catcagcacg gtattgactt
    14821 tgaccttctc aaaaccggcc tcaaatgcag catcgatccc tgccatcacc tgattgaatt
    14881 tatcctggcc ggtaatggca tgaaactggc gggcgtccag actgtcgacg ctgacgttaa
    14941 tgccagtaag tcccgcatcg cgccagttcg ccacatcgcg ttccagacgg taaccattgg
    15001 tggtgaccgc tatctggcga atagcgtcgt tttctcgcac agcggcgatg atatcggtaa
    15061 agtcgcggcg taaagacggc tcaccgcctg tcaggcgcac tttttcggtg cccagactgg
    15121 cgaaggcgcg cgtaacccgg cgaatttcat cgacggtcag aaagccttta ttggtgacgc
    15181 cgctcggttt gtagccatcc ggcaggcagt aggtgcaacg aaagttacac acatcggtaa
    15241 tcgacaggcg caagtagtaa aacttacgcg caaatgcatc agtcagttgt gaagccatgt
    15301 acacctttcc agatacggga ggcgaagtca tttcttcctt cgccctggtg gcaatcttta
    15361 cgagcattgt cacggccaaa gcaccatatc agttgaccca ggtgcagagg ctagagtgtt
    15421 tagtggttaa gccgatacta gcgtgtaaat gagattttta ccatccacac tttcgctatg
    15481 taatcatgta tatagcgtcc tgatcgtgct atttcgctac agaataaggg aaaatggcgg
    15541 ttttactttg atatacatca tcagggaggc atagcgtgca tcgtccttta gaggtagaag
    15601 aaaatagttt gtatcctcaa tattggcagg ttaattgctg tttccccgca atttgcgcta
    15661 ctgtagcgcg gataatctga ctccaggaaa ctatatgcgc aatcgtacgc tggctgatct
    15721 tgatcgtgtc gttgctctcg gcggagggca tggactggga cgcgttctct catcactttc
    15781 gtctttgggt tctcgtttaa cgggtatcgt caccaccacg gataatggtg gctcgacggg
    15841 gcgtattcgc cgttcagaag gcggcattgc ctggggcgat atgcgcaact gccttaacca
    15901 gctgataact gaacccagcg tcgcttcagc aatgtttgaa tatcgtttcg gcggtaatgg
    15961 cgaactttcc gggcacaacc ttggaaacct gatgttgaag gcactggatc accttagcgt
    16021 gcgacctctt gaagcgatca atctgattcg aaatctgctg aaagtggacg cacatttgat
    16081 tccgatgtcg gagcatcctg ttgatctgat ggcgattgac gatcaggggc atgaagttta
    16141 cggcgaggtc aatatcgacc agttaactac gccgattcaa gagttattgt taacgcctaa
    16201 tgtacccgca acgcgtgagg cggttcacgc tatcaatgaa gcggatctca tcattattgg
    16261 gcctggcagt ttttacacca gcctgatgcc aattctgctg ctgaaggaaa tcgcgcaggc
    16321 attacgccgc acgccagcgc cgatggtcta tatcggcaat ctggggcgtg agttgagttt
    16381 acctgcggcc aatttgaagc tggaaagcaa gctggcaatt atggagcagt atgttggtaa
    16441 aaaggtcatt gatgcggtca tcgtcgggcc aaaagtggat gtctcggcgg tgaaagagcg
    16501 gattgtgatt caggaggtgc tggaggccag cgatattccg tatcgtcatg accgccagtt
    16561 gttacatagc gcactggaaa aggcgttaca ggctttaggt taacggaaat ccgaaggaaa
    16621 attccggctt cctaatgaag acaaagtgcg cgttgtttat gacggatgcg gcgtgaacgc
    16681 cttatccggc ctacaaaccg cgctaattca atgtattgca gaatcatgta ggcctgataa
    16741 gcgtagcaca tcaggcaatt ttgcttttgt catctgtctt cttcgttatc ctgtcacgaa
    16801 gccgcgataa acaattcacg cagctgatgc aactggtcac gaatttgtgc cgcttcttcg
    16861 aactccagat tttgcgcgtg ttgcatcatt aacccttcca gctcatggat tttctgttgc
    16921 aacgctttag gcgacatatc catcggcaca ttatccggct caacaatcgg tcgcgatttt
    16981 cctctgccct tcgctttggt tttagcaatg ttctgcccca acgccaggat atcgaccact
    17041 ttcttgttca agccttgcgg cgtaatgccg tgttcctcgt tgtacttctg ctgtttctcg
    17101 cggcgacgtt cggtttcgcc aatcgctttc gccattgacg gggtgatctt atcgccgtag
    17161 agaatcgctt taccgttaac gttacgcgcc gcacgaccaa tagtctggat caacgaacgt
    17221 tcagaacgca ggaagccttc tttgtcggcg tcgaggatcg ccaccagcga aacctccggc
    17281 atatccagac cttcgcgcag taagttgatc cccaccagca cgtcgaactc gcccagacgc
    17341 aagtcgcgga taatctccat acgttcgacg gtgtcgatat ctgagtgaag ataacgcacg
    17401 cgctcaccgt gttcttcgag atattcggta agatcttccg ccattcgctt ggtcagtgtg
    17461 gtgaccagta cgcgttcgtt aattgccgct cgctgacgaa tctccgaaag aagatcatca
    17521 acctgtgtcg ccactggccg cacttcgata atcggatcaa gcaatccggt tggacgcacc
    17581 acctgatcca ccacatcgcc gccggatttt tccagctcat aattacctgg cgtcgccgaa
    17641 acatagatgg tttgcggcgc taatgcttcg aactcttcaa atttcagcgg acggttatcc
    17701 agcgctgatg gcaggcggaa gccgtactcc accagtgtct ctttacgcgc ccggtcaccg
    17761 cgatacatgc cgccaatttg tggaatggtg acgtgagatt catcgaccac cagcagccca
    17821 tcggcaggca ggtaatcaaa cagcgtcggc ggtggctcac ccggtccacg accggagagg
    17881 aagcgcgagt agttttcaat ccccgaacag tagcccagct cgttcatcat ctccagatca
    17941 aactgggtac gctgggtcag ccgctgctct tccagcagtt tgttgttttc caatagcact
    18001 ttgcgtctgg cggccagctc ttctttgatc tcctccatcg cctgtacgat gcgctcgcgc
    18061 ggtgtgacgt agtgcgtttt cgggtagatg gtaaaacgtg gaatggtgga aacaatctgc
    18121 ccggttagcg ggtcaaataa cgacaatcgt tccacttcct cgtcaaacag ttccacgcga
    18181 agtgcaatgt catccgattc tgccgggaag atatcaatca cctcgccacg aacacggaaa
    18241 gtaccacgct ggaatgcttg atcattacga gcgtattgca gctccgccag tcggcgcaga
    18301 atcgcgcgct gatcgataat cataccgacc gtgagatgga gcatcatctt gagatataaa
    18361 tcaggatcgc ccagaccata aatcgcggaa acagacgcga ccacaacaac atcacgccgc
    18421 tccagcatcg ctttggtggc ggacaaacgc atctgttcga tatgttcgtt taccgaggca
    18481 tctttctcaa tgaaggtgtc ggaactcggt acataggctt ctggctgata gtagtcgtag
    18541 taggagacga aatattccac cgcgttttcc gggaagaact ctttcatttc gccatacagc
    18601 tgggccgcca gcgttttgtt gggcgcaagt accatggtag ggcgctgaag gtcagcaatg
    18661 acattggcaa tggtgaaggt tttcccggaa ccagtcacac caagtaacgt ctggtgcgcc
    18721 aggccatctt ccagcccctc ttcgagacgt cgaatcgcct ctggctgatc gccagaaggt
    18781 ttaaaagcgg aattcagttt gaacggttta ctcatgagtc gctacctgaa ggagttgggc
    18841 gggcaggtat gtaattttac tcgtcgtact taattatgcc aacaaattat actggat
//
LOCUS       DBFIFM010000077        18852 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.077, whole genome shotgun sequence.
ACCESSION   DBFIFM010000077 DBFIFM010000000
VERSION     DBFIFM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.077"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(187..4089)
                     /gene="hrpA"
                     /locus_tag="N7C74_002354"
     CDS             complement(187..4089)
                     /gene="hrpA"
                     /locus_tag="N7C74_002354"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HGU2432215.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRIGHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWFNKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4290..4895
                     /gene="azoR"
                     /locus_tag="N7C74_002355"
     CDS             4290..4895
                     /gene="azoR"
                     /locus_tag="N7C74_002355"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HGU2432216.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTTRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(4946..6238)
                     /gene="ynbD"
                     /locus_tag="N7C74_002356"
     CDS             complement(4946..6238)
                     /gene="ynbD"
                     /locus_tag="N7C74_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HGU2432217.1"
                     /translation="MLQGAGWLLLLAPFFFFTYGSLNQFTAVQDFNSHDIPSQVFGWE
                     TAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCGFFLYPLKF
                     SFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIVLCWLLWRHFRQHLAVRWRKVCGGW
                     FLLIAISMLTTWQHHFIDVITGLAVGMLIDWMIPVDRRWNYQKPDQRRIKIALPYVVG
                     ACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQGKLPPAVYW
                     LTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTFEFPRGRAT
                     KDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLL
                     CYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(6252..8009)
                     /gene="ynbC"
                     /locus_tag="N7C74_002357"
     CDS             complement(6252..8009)
                     /gene="ynbC"
                     /locus_tag="N7C74_002357"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HGU2432218.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLARSVRDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYHRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPGNEQVKNSLAGLAN
                     AIEPGGILLYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8025..8921)
                     /gene="ynbB"
                     /locus_tag="N7C74_002358"
     CDS             complement(8025..8921)
                     /gene="ynbB"
                     /locus_tag="N7C74_002358"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGU2432219.1"
                     /translation="MLEKSLATLFALLILATLINRFRVWRLPERKGDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGFSC
                     FELFLLFIPLAGFLILTTWRVLVGDTSGFLHTVSAIFWGWIMTVFALSHAAWLLMLTT
                     TNTQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAVKRDVGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(8921..9526)
                     /gene="ynbA"
                     /locus_tag="N7C74_002359"
     CDS             complement(8921..9526)
                     /gene="ynbA"
                     /locus_tag="N7C74_002359"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGU2432220.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLITGL
                     LLMLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYVGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(9697..12003)
                     /gene="ydbD"
                     /locus_tag="N7C74_002360"
     CDS             complement(9697..12003)
                     /gene="ydbD"
                     /locus_tag="N7C74_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HGU2432221.1"
                     /translation="MFKPRLCSWIGLLPLFMLSLPVQAELRCVANAVDIETFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRLKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDGQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSHLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNDVVTSLALKLAEMKHHGQLLRM
                     TPQESDKIAVYLYQKFENDDDLIGALFLALPDNLQFNFVKRMEKKSPAYFCCRDMQII
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSDAYETS
                     ADMILSELISYGEVDDQMLLNATSLIRSDDWDFLESALISWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVNEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALKLVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(12067..12927)
                     /gene="pdxI"
                     /locus_tag="N7C74_002361"
     CDS             complement(12067..12927)
                     /gene="pdxI"
                     /locus_tag="N7C74_002361"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HGU2432222.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIEALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEKV
                     LSTLDGISRE"
     gene            complement(13147..15606)
                     /locus_tag="N7C74_002362"
     CDS             complement(13147..15606)
                     /locus_tag="N7C74_002362"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HGU2432223.1"
                     /translation="MNEGGALTNEGVLNTVTLEGGTFNNNGTLNDVVKIEKNSNAVIN
                     NTGSLSTLQLHDGTVNNSGIASARVNAQGDAVFNNLAGGEARKGAILYNSAVVNNAGT
                     WKMGYQDENNNAGTLDIDDKSTFNNSGKLILDNSKNAIRFQGSNANATLYNTGEMTLD
                     AALGAGAIHYDDGASEFINKGVVDAKVTVAVSTAGATESDAFLWNQDGGVINFDKDNA
                     SAVKFTHNNYVALNDGVMNISGNNAVAMEGDKNAQLVNNGVINLGTEGTTDTGLTGMQ
                     LDANATADAVIENNGTINIFANDSFAFSVLGTEGHIVNNGTVVIADGVTGSGLIKQGD
                     SVNVEGVNGNSGNNTEVHYTDYTLPDMPNTYTTSPFSETTDSGSSDGSSNNLNGYIVG
                     TNVDGSAGKLKVNNASMNGVGINTGFAAGTADTTVSFDNVVEGINLTDADAITSTSVV
                     WTAKGSTDASGNVDVIMSKNAYTDVATDASVNDVAKALDAGYTNNELYTSLNVGTTAE
                     LNSALKQVSGSQATTVFREARVLSNRFSMLADAAPKVGNGLAFNVVAKGDPRAELGNN
                     TEYDMLALRKTVDLSESQSMSLEYGIARLDGDGAQKAGDNGVTGGYSQFFGLKHQMSF
                     DNGMRWNNALRYDVHNLDSSRSVAYGDVSKTADTDVKQQYLELRSEGAKTFEPREGLK
                     ITPYAGVKLRHSLEGGYQERNAGDFNLSMNSGSETAVDSIVGLKLDYAGKGGWSANAT
                     LEGGPNLSYSKSQRTASLAGAGSQHFNVDDGQKGGGINSLASVGVKYSSKESSLNLDA
                     YHWKEDGISDKGVMLNFKKTF"
     gene            complement(15826..>18852)
                     /locus_tag="N7C74_002363"
     CDS             complement(15826..>18852)
                     /locus_tag="N7C74_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed virulence protein BigA"
                     /protein_id="HGU2432224.1"
                     /translation="KKLLSVCVAMALSSQTWAADTSIPDSTEKTRKSSKITCPANIHS
                     LSKEQLENLSAECRENKDSAVLPWAAAGLAAVATGIAIYTLSDDDNHHHNNSPVPDDG
                     GDTPVPPDDGGDTPVPDDGGDTPVPPDDGGDTPVPPDDGGDTPVPPDDGGDTPVPPDD
                     GGDTPVKHNPVVYKNGVTWDQDAKTVKIRETTFTYSQNADGSYTLTAPEGKETIVKSW
                     TVHDDSNTVVFDGVNTSGGITWSYDDDGLIHITKEAGVVVDGTTGNAIEFGNAIITDQ
                     GGNTALNGGTVMTVDGDNISLNNDGKTTAIGEGSVVGILTGDNITINNNGETEVDGGT
                     AVIINGDNTKLNTAGDSTITNGGTGSLINGDNARVDNQGTMSVDGENSTGSKIVGDGA
                     TIKQEGDLYVSGGAHGIDVDGNDTFVSNKGNITVIEDNSIGMLLDGDGVSVINMGDLN
                     VGQAAAGENAIGIQIDGDNATFVNVGDISATNAGTGVSVAGDKANISLAGGLDVGDFS
                     TGLDVSGNNNNMTLATYELNVTGQKATGVNVSGDGNTIEIAGSILVDKDQKADNAQPY
                     FFNPSTGVNISGDNNDVTLDGQLTVVADSKTTSRSYASYDGAQEHIAGIVIAGDDNTF
                     TLNGGVHFVGEKNVMDDGSKPSASRRGIGDTPLINVDGHSPVYLNGESTISGEFPLGF
                     ENLIQLSHGAELEIGADATFDMSDVDSFTYYYRVALSTISIDSGAKATNNGEVELKNI
                     GFAAAWNKDSTVINNGSIGLAMYDFGTDPAPKVFDVEYGGIGVNNGTMTTKMMNQHSV
                     LNYPAEWNLSDGTSFNNKALGLTGMLASYSSSILNGETGIIDMYGRGSVGMLAIDKST
                     ADNEGQITLDTLWVDENDETSLRNNVANSTAKDFGVGMASGTDAYNGALTKATATNHE
                     NGVITVYNAGAGMAAYGNTNTVINQGTINLEKNENYNDSLGVNKLVGMAVYHEGTAIN
                     DQTGVININAENGQAFYNDGTGLIINYGTICTFGVC"
BASE COUNT         4733 a         5081 c         4272 g         4766 t
ORIGIN      
        1 cgttcaaatt gcgacctcag taaaatatca tgtctactca ttgcaatacg tcctgttcgc
       61 ccaaggcgaa gtatgaatga aacttatgta aaatagtacc gcccttgcta tatttcagaa
      121 ttcataaaga aaaaacccgg cgctaaatgc ttaccgggtt ttagatttat caggcaaata
      181 gcagggttaa ccgctaatct gctccattgc ctgcaaaata cgcttatctg aaatcggata
      241 aggcgtacca agttgttgag cgaagtaact aacgcgcagc tcttctatca tccaacggat
      301 ctctttcacg tcttcatcct cacgacgtgc aggcgggagt ttgttgaacc attgctgcca
      361 cgcctgctgg acgttttcga ctttcagcat ctgtgcacga tcgcgatgtg gatcaaccgc
      421 cagtttttcc agccgttttt caatcgcctg caaataacgc agcgtgtcgc ccagccgttt
      481 gaagccgtta ccagtgacaa aaccgcgata taccaacccg cccatctgcg ctttaatgtc
      541 agaaagcccc agcgccatgg tcatatccac ccgccctttc agacgtttgt tgatattgaa
      601 cactgccgta aggatttgct cgacctgctt cgcaatatcc accaccgtgt cgttcagttc
      661 ggcacgcact ttttcatgca gcgcagcaaa gccttcttcc gtccagaccg ggcctccatt
      721 ggcgtcgatc agttgatcca caccgcatga gatacagtcg tcgatcagct ccagcacttt
      781 gccatacggg ttaaagtaca gccccagctt ggctttgttt ggtaacttct catgtaaata
      841 cttgattggt gagggaatat tcagcagcag tagacggcga agaccgttcc acattgcctg
      901 tttttgctcc agcgggttat caaacagttt gatcgccaca ctgtcgcgct catccaccag
      961 cgccggccac gccttcactt tgtagttacc acgcttctgt tcgtagcttt ccggcaactg
     1021 accaaaactc cagatatgta agccgctctg ctcgataccg tcatccgcca ccgcagatag
     1081 cgtttcctgc actttgcctt tcagcgcatc tttcagatct tgtagcgagc gcccttcttt
     1141 tagcttcttg tttttgtcat ccaccacgcg gaaggtgatt ttcaggtgat cgggcacctg
     1201 atcccagtgc cagtcttcgc ggtcaacggt aacgccggtc atccgccgta actcgcgctc
     1261 aaggctgtcg agcaacggta actccagcgg tgtgacgcgg cctaaaaacg cttcggcata
     1321 gtttggcgca ggtacaaaat tacggcgtac cggtttcggc aacgatttaa tcagagcaat
     1381 caccagttcg cggcgcagac cagggatctg ccattcaaac ccgctttcct caacctggtt
     1441 aagtaacggc agcggaatat gtacggtcac accgtcagca tccgcgccgg gctcaaactg
     1501 atagctcaaa cgcagcttga gattgccctg atgccagaag ttcgggtaat ccagcttgct
     1561 gatcttttcc gccccttctt tgatcaacat gctcttttca aagttgagca aatcaggcgt
     1621 ttcgcggctg acttttttcc accagctgtc gaagtgacga gcggagatta catcgtggcc
     1681 gatgcgctgg tcgtagaact caaacagcgt ttcgtcatca accagaatat cgcggcgacg
     1741 tgatttgtgt tcaagctctt ccacttcggc ccgcagtttc aggttttcac ggaagaatgc
     1801 gtgacgcgtc tgccagtcac cttccaccag cgcgtggcga ataaagagtt cacgacataa
     1861 cgccggatcg atctggctgt agttgacctt gcgcgcggca acaatcggca aaccataaac
     1921 agtgactttt tccgttgcca tcaccgcgcc ctgcgcccgt tcccagtgcg gttcgctgta
     1981 ggtacgttta atcaaatgct gagcgacagg ctccacccat tccgggtcga tacgcgcagc
     2041 aatgcgcccc cacaggcggc tggtttctac cagttccgcc accattaccc atttcggcgg
     2101 ttttttgaat aaaccagaac cggggaagat ggagaaacgc gcgttacgtg cgccggtata
     2161 ttcttgttta tcggcatctt tcatgccgat atgggaaagc aaccccgtca gcaacgcaat
     2221 gtgaatttca cgatactccg ccggttcgct gttcaccgga atgccgagtt ctttcaccac
     2281 ctggcgcaac tgggtgtaga tatcctgcca ttcgcgcaca cgcagatagt tgagataatc
     2341 ggtacgacac aggcgacgga aggcgttgga agaaagcgcc ttttgctgct cgccaagata
     2401 attccacaga ttcacaaacg cgaggaagtc agactctttg tcgtggaagc gacgatgttt
     2461 ttcgtccgat gcctgctgtt tgtccatcgg acgttcgcgc ggatcctgaa tggagagcgc
     2521 agacgtgata atcatcgcct cacgcacgca gccatgtttt tgcgcttcca gcaccatacg
     2581 cgccagacgt gggtcgacag gcaactgcga gagctggcga ccgagcggcg tcagtttata
     2641 ggcgctggcc tgttcatccg tggtgatcgc gcccagttct tcgagcagac gcacgccatc
     2701 ctggatattg cgtttatccg gtgcttcgac aaacgggaac gcagcgatat cgcctagccc
     2761 cagcgcggtc atctgcaaaa taaccgaggc caggttggta cgcagaatct ccggatcggt
     2821 aaactccggg cgcgagagga aatcgtcttc ggaatagaga cgaatacaga tcccttcgga
     2881 cacacgacca cagcggcctt tacgctgatt ggcagacgcc tgcgaaatgg gctcaatcgg
     2941 caaacgctgc actttggtgc gatagctgta gcggctgata cgcgccgtac cggggtcgat
     3001 aacgtattta atccccggta cagtcagcga ggtttccgcg acgttggtcg ccagcacaat
     3061 gcgtcgcccg ctgtgcgact ggaacacgcg gttctgttcg ctattcgaaa gccgcgcata
     3121 aagcggcaag atttcggtat ggcgtaagtt cagcttgttc agcgcatcgg cggtatcgcg
     3181 aatttcccgc tcgccgctca taaagatcag aatgtcgccg gggctttcct ggcttagttc
     3241 gtccacggca tcgaaaattg cctgcaactg gtcgcgctca gtgtcgtcgg cttcttcaac
     3301 aatcgggcga tagcgcactt ccaccggata ggtccgaccg gagacttcaa taatcggcgc
     3361 attattaaag tggcgcgaaa agcgttccgg gtcgatagtc gcggaagtga taatgatttt
     3421 taggtcagga cgccgcggca gcaactcttt caaataaccg agcagaaaat cgatattcag
     3481 gctacgttcg tgcgcttcgt caataatgat agtgtcgtac tgcatcagca ggcggtcttg
     3541 ctggatctcc gccagcagga taccgtcggt cattagcttg accatcgtgt tatcacttac
     3601 gtgatcgctg aaacgcactt tgtaaccgat gcaaccgccc ggctccgttt tcagctcttc
     3661 cgcaatacgg ttcgctaccg ttcttgccgc caggcgacgc ggctgggtat ggccgatcag
     3721 ccctttaatc ccgcgcccca gctccataca gattttcggt aactgagtcg ttttaccaga
     3781 acccgtttcc ccggcgacta tcaccacctg gtgatcacga atcgcttcga gaatgtcctg
     3841 ttttttctga ctaaccggca aattgtcagg ataagtaatt tccggtcgtg ccgcttcacg
     3901 cagcaggact ttccctgccg cctggtcaat ctctttcgcc atctcctgga aaatggcctg
     3961 ttgtgcatca ggatttttaa ccttcttcac gccgtgcaga cggcgagaaa aacgcagtct
     4021 gtcacgcagc atcagcgaat ccagccgctg ctgcaaggcc gtaaaagtca atttttgttg
     4081 ttctgtcata acgttagtgg gcagtagtct gcctgattaa ttttctttga aatgatagat
     4141 atagagtacc acatcgcggc tttctatgcg ttgttcaata aattcgaaca taggcttcga
     4201 tttattgcgc tatctctgtg gcaggattgt gaataaagtg tcaacaagca acggggcatc
     4261 gcccattcaa atatctataa ggaaacacca tgagcaaggt attagttctt aaatccagca
     4321 tcctggcagg gtactctcag tctaatcagt tgtccgatta ttttgttgaa caatggcgcg
     4381 aaaagcactc cgctgatgaa atcaccgttc gcgacctggc tgcaaatcca attccggtac
     4441 tggatggcga actggttggc gctctgcgtc cgagcgatgc gccgctgact acgcgtcagc
     4501 aggaagctct ggcactttcc gatgagctga ttgccgagct gaaagcccat gacgttatcg
     4561 ttattgcggc accgatgtat aacttcaaca tctcgactca gctgaaaaac tattttgacc
     4621 tggttgcacg tgcaggcgtt actttccgct ataccgagaa tggtccggaa ggtctggtaa
     4681 cgggtaaaaa agccatcgtt attaccagcc gcggtgggat ccacaaagat ggaccaacgg
     4741 acctggtgac gccgtatctg tccacgttcc tgggctttat cggcattacc gatgtgaaat
     4801 ttgtcttcgc cgaagggatc gcatacggtc cggaaatggc agcgaaagcg cagtctgacg
     4861 cgaaagcagc catcgacagt attgttgctg cataaccgga cacgatcttg atgtggactg
     4921 tagttcagtc cacatctcaa tccacttacc tgttttccca taatttcaac atcgctttgt
     4981 gctcttcctt aagcacaata tgcgagcgtc tggctcgaat aaagctaatc gcttcatcaa
     5041 ctgttttaca gtgtccgtaa cataacagcc atgccgccac taccagcgca ctgcgcgata
     5101 atcccaatgc gcaatggacc agaacgctgc cttgctcttc gcgtaatgtt tccagcatcg
     5161 ccacggcctg tcggagctcc ccctcttccg gaaccaccag atccagcatc ggtacacaga
     5221 aatagagtcg atcttttgtc gctcgtccgc gagggaattc aaaggtgaca tccagaaccg
     5281 cattctgtgc cggaatatgt cgtggaaacg cccctaaata aacaccagca gtcattttgc
     5341 tcaccggctc caggcgacga caaaaccaac gcatagatag ccacatcccg atgcgccagg
     5401 gcaatgtcag ccagtaaacg gcgggcggga gtttcccctg actatctttc cctgttgtta
     5461 tcgcgccaag cccaccgtac ccacggccaa taatgagtag cgataatact ggccaacata
     5521 accagactga ccaccataac tgaaccatca tcattagctc catcaacaca atgcacgcgc
     5581 acgcgcctac gacatatggc agtgctattt tgattcgacg ttgatcaggt ttctgataat
     5641 tccaacgacg gtcgacgggt atcatccagt caatcaacat acctaccgcc agccctgtga
     5701 tgacatcaat aaaatgatgc tgccaggttg tcagcatcga aatggcgatg agtaaaaacc
     5761 atccgccgca gactttacgc cacctcacag ccagatgctg acgaaagtga cgccaaagta
     5821 gccagcagag aacaatatgc agcgaaggag actggttata aggcagatca aacagttcaa
     5881 gttgcgaaaa tagccatcct gtcaccccac tcacttcagg acggataaaa ctaaacttca
     5941 gcgggtagag aaaaaaaccg cagcaggcca ttaccgttgc cagaataagc cggtggacaa
     6001 gtcggcgctg ttcgaatgtc gagctacaaa cgaacagcga aaatccatat aaaagatcca
     6061 gactccagta agggacaata gtccagggaa gaaaagggat cgccgtttcc cagccgaata
     6121 cctgactggg gatatcatgg ctgttaaagt cctgaaccgc ggtgaactga tttagagatc
     6181 cataggtgaa gaagaaaaac ggggccagca acaataacca gccagcgcct tgtagcaata
     6241 cgttccggcg ttcagttatc acgacgcacc gccatcgaaa ccgtaaaaat gccccactca
     6301 tcaatccgtt gtgtgcattt atcaaatccg gcatcacgca cgagtgaatc catctcccct
     6361 tgcgaacgca cgcgcattac ccacggttta ccatctttat gactggttaa caccccggca
     6421 atcagctcca gttgtgggtg ccacggctgc ccggtgtaga ggagaatgcc gcccggttcg
     6481 atggcattgg caagacctgc gagtgagttt tttacctgct catttccggg aaaaagctca
     6541 tacagtccag agacaatcgc cagcgtaggc cgcggagtta acgcgctgag ttcctccggg
     6601 ttaaacgcat cgccctgttc aaaacgcacc cgcccagaca ttccccgttg agcaatcatc
     6661 tcttgcccct gtgcaacatt taactcgctg taatcacgta acaaaatatc gcttacggca
     6721 ggctcgtttg ccagcgcatc cagtacatag cgcccatgcc ctgcggcaat gtcaaccacg
     6781 cggacggcta aacctttggc gtggagatcg gcaacggctt gtttaatcag tatttgtaaa
     6841 tgggttttgc gctggcgaat accgcgccag ccaacactgt tcaggtagat tttgtcgatt
     6901 aagcgcccga atgcgttact accttgcggt tgattttgat agacataatc cagcgaactg
     6961 cctgaatcaa agccggtgct cattccgaga tgcaggcccg cagagtgcgt cccgaacaat
     7021 ttcatcgctt tacgcataaa gcgatacgcc aaatcaaccg gcgataatgg cacgggtcca
     7081 cctgaaagga gccgccagcg atccgctgat ggtccagtgc ggtcttcatg ctgataatca
     7141 aatttttgtg acttgttagc atataaacga ctaataaagc tttgcatttt ttcaaatgcc
     7201 tgcgccctgt tctcttcacc caacgtgtcg tgataaaagc ctggcagcag atgcagctct
     7261 ttcagagggc tacgtaatct atgataaaaa tcaatctgcg gttggcgatg caccacatag
     7321 tcatcgcctg atatcagaag ttgcgtgggg agcgtaatcg ccgccgcatc gctaacaata
     7381 cgttcagacg ttttataaag atcgagcaag atattaacgg caatcgcccg tgtgatcagc
     7441 ggatcattat tgaaactcgc cacccgttgc cgatcgtggg tcaaatagcg tcctttcaca
     7501 taggaattaa taaaaaacag accacgcaga cgatgccata acgccagcgc aggacgtgcc
     7561 agcggcacat acaatttaac cttaaaggcc ggagaagcca gcaccagccc gcgaattgca
     7621 ggcgcataat catgaaccca tgtggcaacc agcactgcgc cgacgctctg cgcgatcact
     7681 accacctctt ccagcccgac ctggctgtcg ctggcagcaa aacggacaaa ctcatcgaca
     7741 tcccgcactg aacgcgcaag agatgggcta taaccacggg gccccgaagt ttgtccatgc
     7801 cctcgggcat cccatgcata aaaagcagta tctggcatcg ccagttcatc aacgatatgt
     7861 tgtagacgac cagaatgttc atgaccgcga tgaaataaga cgatgacctt tttcgcccct
     7921 ggctgtaaag cgggccagtg ccgataaaac aacgctgtat tatcactggt ggtaaaaaaa
     7981 tgttccccag ggatgcgtga attttccatt ttctctattt tccttcagta acagcagtag
     8041 cgaataaaat aaaaaaatac cggagcggtg aaaattaatg agtcaatcct gtcaaggagt
     8101 ccgccatgtc ctggtaatag ttttccgcta tctttaacac ccacatctcg tttgacggct
     8161 gacatcacta catcgccgca aaaaccacta ataccaatta acaaacccgc taataatgcc
     8221 tgtaatgtat ttagcggtgt cagcaacggt ccaataatca gtgacgcaat catggtggtg
     8281 attacgcccc ccaccagacc ttccagcgtt tttcccgggc tgactttggg gaccactttt
     8341 cttctgccgc aggatttccc ccataaatac tgtgcaatat cgtttgactc tgttaacgcc
     8401 aaaagaaata acaccagtaa cgccccgccc tgggtatttg tggttgtcaa cattaataac
     8461 caggcggcat gactcaaggc gaaaacggtc attatccagc cccaaaaaat ggcgctcacg
     8521 gtatgcagaa aaccggaggt atcacctact aaaactcgcc aggtggttaa tatcagaaac
     8581 ccagccaaag gtataaataa cagaaacagc tcaaagcaac taaaaccgat aagcaaatag
     8641 ttaagaggaa taacccaata taaccaacgc ggaaaatgta cggatataag cgtacaatat
     8701 tctttcaatg ccagaaaact tatcagcgca aaaaacgtca acgtcatcca gcgtggacct
     8761 gaaatcacca gtgaaaaaca aatgacgata ccccaccagg tacgaatacg tagtgtaact
     8821 tcatcccctt ttctctccgg taaccgccat acccgaaaac ggtttatcag cgttgctaaa
     8881 attaacaacg cgaaaagtgt tgccagagat ttttccagca ttatctttca gcactcatca
     8941 ggagcacact ccgacaacga ttaatcgcag tccagagaag cagaattgaa gcaatgctcc
     9001 ataaaagatt attccattgc atccactgtg gataaatggc aacggccagc ccccacaacc
     9061 caaatattaa cgcgcgatcg cttttgccaa aaggaccgac ataactacga ataccgttaa
     9121 tcgtctgcgc gagtaaaccg caaaactcag tcaatatggt gcaaaataac atgagtatta
     9181 cgagtgatgc gttactttcc ggtaaaaata aaaacggtaa atagagcgca atatcggaaa
     9241 taacatcgcc agtttcattt aaaatcgccc ccagacgtgt ttgctgattg cactcacgcg
     9301 ccaacatgcc atccagcgca ttgagtgcca tacggataaa aagcacgatg ggcaatagca
     9361 aaaagaggat gggttgtgcg accaacatca acagcaatcc ggtaataaga gaaagcgcca
     9421 gtgcagcaag agtgatgtga ttcgctgtaa catggtgctt ataaagccaa aacatcgtcg
     9481 gccttaacag cgactgaaag agcggtttta tctggtagag cgtcattatc aatccttgat
     9541 aattcggttt gcgctatggt gcatcaattc ccctgacgga gatatcgcaa cacgacgctt
     9601 cattaaaaat tcagaatata tcaaggtatt ggggactcac tgtcaggaat gattatcccc
     9661 aataaatcaa gtggttaaaa attattacgc tgttattcaa tacaactcat caagttttct
     9721 catcgcttgt tcccgtattg cgcgcgtctt accgttaagc gccagctggc gaactaaatc
     9781 cagttcaggg ttaacaagct ttaacgccag caaaggatca cgtttcagcc tcgcctttaa
     9841 tacatcaact ggaaaacggg gattattcat cgccacaatc caccactcgg gatcaatttc
     9901 tgccgccagt actgcaggcg gtgtgtggcg gttgttaagt gcgccgaact tgaaaaatgt
     9961 gagattgctt ttcgccagtg tactccacca tatacgatcc ggttgttttg ccagtacatc
    10021 cagttgtgct aacgcatcgg ggtctagtgc ataataaaca cgtaacgctt cgttaacctg
    10081 cgcacgggaa ctgttttcct gcctcagaaa aaacttcgcc caaatatcat tgcgtggcgt
    10141 gttttgctgt aattccttga gaacaacagc gggtaaatta tcccaactaa ttaatgcact
    10201 ctctaaaaag tcccagtcgt ccgaacgtat cagagaggtg gcatttaata gcatctggtc
    10261 atcaacttca ccgtagctga taagctcaga caaaatcata tcagcgctgg tttcatatgc
    10321 atctgaatct gctttgggat tatttttccg atgcgataaa gcacgttgcc aaattttttg
    10381 tttcatcgac gtgctcagat actgattttt cgccagatta ctccacccct caggatcgtt
    10441 gaaacgtgtc aataaccgtt gtaaagcagc gtcagagtga attatctgca tatcccgaca
    10501 gcaaaagtag gcaggggatt ttttctccat ccttttaaca aaattaaact gtaagttatc
    10561 tggcaacgct aaaaataacg cacctattag atcatcgtca ttctcgaatt tttggtataa
    10621 atatactgct attttgtcac tctcttgtgg cgtcatccgc aacaattgac catggtgttt
    10681 catctctgcc agctttaacg ctaagcttgt gacaacatcg tttgatgaat cgttcgccaa
    10741 ctgcattgcc tgattgtagg tgagttgtcc tccagcaccg acctgacgaa tagaggaatc
    10801 atcactgcgg atatagccat caataatgga ttcgggcaat aaccacgcat ctttataaag
    10861 ctcctgaatg atcatatccc aatctctttt cgccagagtc atgacgatat ccagagggag
    10921 attttcacgt agcgcaagat tcaacggacc gacaccatca ggtagatgac tcattcgcgt
    10981 cattatttcg acagagagcg gttcacggtc gagcaacccc agaacaacag gctggtaacc
    11041 caattgctga tcggtcaact ccagaatctc attgtcaggc atatatctcg ccatactttc
    11101 aatcgtttgt ccatcattgc tattaataag ttgtttgcgt aggttagctg aaattccttt
    11161 aagccttggg agagttcgca ttatggcaag atcaaatgtc gcaatatctc gtccttcaaa
    11221 tgcagcaacg ttgccttcat ccagcatttg caattcctgc actaactcag gagaaggctt
    11281 aaggcgaaac agcgcttctt ttttcatctg attacagaca ttccctgcca tgcgaatatt
    11341 gcttcgctta aaaatttctt tcagagcatc ttcatcattt attagacccc aataatatgc
    11401 atcaggattt attatcatca gcgctttttt gacattttca gtgtcatttt tgtcactcgc
    11461 acggaccatg aattcactat ttgtcgaaga cgcaacaaca ggtgcaatta tgtcattgat
    11521 aaatatccca cgcgcattaa tactcccctt aatcgtagac ggattatcgt aatatgagtc
    11581 gataaatacc agtccacttg catttatcga accggtaatt aacagtggtg aatccgtaac
    11641 tatattcgtt gccgtcacgt taccgagaat aacccatgga ttagaaagag agaaagtgga
    11701 aatattacct ttgactgtca aattgccaag aacaatcaat ttttgattac tctctatatt
    11761 accttctacc actaaatcgc cacgatgcac tttccagtcc gatgcagata aaccgaaggg
    11821 gacaagattc acgctactgt tgatagcttg ttcaacttgt tgtttatctt cagcggtagc
    11881 cgcagaaaaa aaagtttcaa tatcaactgc atttgcaacg catcgaagtt ccgcctgtac
    11941 tggaagactc agcataaaca ggggaagtaa acctatccag ctgcaaagcc tgggcttaaa
    12001 catatgcact ccatgccatt aaaaaagaaa caaaagggaa atgcatcttt cccttttgta
    12061 tattcgttat tctcgcgaaa taccatccaa cgtagacaac actttctcag aaagatgcaa
    12121 cttttcagca gccatattct cccgtaaatg cgcaacagaa gacgtccctg ggatcagcaa
    12181 aatattcggt gaacgttgta acagccacgc cagcgccacc tgcattggtg ttgcacccaa
    12241 gctcgcagca acatcggaaa gcgtggaaga ttgcagcggc gtaaagcccc cgagcggaaa
    12301 gaacggcacg taggcaatgc cgtcacgagc caaagcttca atcattgcgt catcagcacg
    12361 atgagcaatg ttgtattcgt tctgcacaca gacgatttcg gcaatcttgc gcgcctctgc
    12421 aacctgcgtc ggtgtgacgt tgcttaggcc aatatgtttt accaggcctt gttgttgcat
    12481 ctctgccagc acggtcaggc tggcttcaat cgatccttcc gccgggccat gaccatcccc
    12541 catcatcacg cgcaggttaa ccacatccag tacgtccagg ccgagattac gtagattatc
    12601 gtgcaccgct ttttgcagct ctgccggaga aaatgcgggc aaccaggatg cgtcctctcc
    12661 acgccgcgca ccaattttag tgacaattgt cagatcgtca gagtaaggat aaagcgcttc
    12721 gcggataatc tgattggtga cgtgcggacc ataaaagtcg ctggtatcaa tgtgattgac
    12781 gcccaatgcc agcgcctcac gcagcacggt tatagcgacg tgacgatctc ttggggggcc
    12841 aaaaacccca ggacctgcca gttgcatcgc gccataacca agacggttaa cggattttgt
    12901 accgagagta aatgtattgc tgctcatcgc accctgccct gtaataaaac ggattaagaa
    12961 aaatcagtat agaaacgaaa agagataaat aatgcgaagg cgtaaacaag atttctggat
    13021 gagtagcact attgtcctga aagacaccaa aacattagac agcaaaagat aagtcaagcg
    13081 tcgcatccgg caataaaaaa ccgcaacctg tatcagcgtt gcggtttgtg tatcacatgc
    13141 taaaaattag aacgttttct taaagttcag catcacgcct ttgtcgctga tgccgtcctc
    13201 tttccagtga tacgcatcca gattcagcga actttctttg ctactgtact tcacgccgac
    13261 gctcgccagg ctgttgatac cgccgccctt ctgaccgtca tcgacgttaa agtgctggct
    13321 gcctgcccct gcaaggcttg ccgtgcgctg gctcttgctg tagctcaggt tcggcccgcc
    13381 ttccagcgtc gcattcgcgc tccagccgcc tttccctgcg tagtccagtt tcagcccgac
    13441 gatgctgtcc accgccgttt cgctgccgct gttcatgctc aggttaaagt ctccggcatt
    13501 gcgctcctga tagccgcctt ccagcgagtg acgcagtttc actccggcgt acggggtgat
    13561 tttcagccct tcgcgcggct caaaggtttt cgccccttcg ctacgcaact ccaggtactg
    13621 ctgtttcaca tccgtatccg ccgttttgct gacgtcgccg taagcgaccg agcggctgct
    13681 gtcgagatta tgcacgtcat aacgcagcgc gttgttccaa cgcataccat tgtcgaagga
    13741 catctggtgc ttcaggccaa agaactggct gtagccgccg gttacgccat tgtcgcccgc
    13801 tttctgcgca ccgtcgccat ccagacgcgc gataccgtat tccaggctca tgctctggct
    13861 ttcgctcagg tcaacggttt tacgcagtgc cagcatgtca tactcggtgt tatttccgag
    13921 ttccgcacgc gggtcacctt tcgccaccac gttaaaggcc aggccattgc ccaccttcgg
    13981 cgcggcatcc gccagcatgc tgaagcggtt gcttaacaca cgcgcttcac ggaataccgt
    14041 ggtcgcctgg ctaccgctca cctgcttcag ggcgctattc agttcagcag tggtgcccac
    14101 gttcaggctg gtatacagct cgttattggt gtaacccgca tccagtgcct tcgccacatc
    14161 gttcaccgaa gcatcggtcg ccacatcggt gtaggcgttt ttgctcatga tgacgtcaac
    14221 gttgccgctg gcatcggtgc tgcctttggc ggtccatacc acggacgttg aggtgatggc
    14281 atcggcgtcg gtcaggttga tgccttccac cacgttgtcg aaactgaccg tggtgtctgc
    14341 cgtaccggcg gcgaaaccgg tgttgatccc gacaccattc atgctggcgt tgttgacctt
    14401 cagtttaccg gcgctgccgt caacgttggt accgacgata tagccgttga ggttgttact
    14461 gctgccatcg ctactaccgc tatcagtcgt ttcactgaaa ggtgaagtgg tgtaagtgtt
    14521 tggcatgtcc ggcaacgtgt agtcggtgta atgcacttcg gtattgttac cgctgttgcc
    14581 gttcacccct tccacattca cgctgtcgcc ctgcttaatc aaccccgaac cagtcacgcc
    14641 gtcggcaatc accaccgtac cgttgttaac gatatgacct tctgtgccca gtacgctgaa
    14701 cgcaaacgag tcgttagcaa aaatattgat cgtgccgttg ttctcaatta ccgcatcggc
    14761 ggtggcattg gcatccagtt gcataccagt caatccggta tcggtggtgc cttcggtacc
    14821 gagattgata acgccgttgt taaccagctg tgcgttttta tcgccttcca tcgccacggc
    14881 gttgttgccg ctgatgttca ttacaccatc gttgagagca acatagttgt tgtgggtgaa
    14941 tttaaccgcg ctggcgttgt ctttatcgaa gttaattacc ccgccatcct ggttccacaa
    15001 gaaggcatcg ctttctgtcg caccggcagt acttaccgcc acggtgactt tcgcatccac
    15061 cacgcccttg ttaataaact cgctggcgcc atcgtcgtag tgaatagcgc ccgcacctaa
    15121 tgcggcatcc agcgtcattt caccggtgtt atataacgtg gcgttagcat tgctgccctg
    15181 gaagcgaatg gcgtttttgc tgttatcaag gatgagtttg ccgctgttgt tgaacgttga
    15241 cttatcgtca atatccagcg tcccggcatt gttgttttca tcctgataac ccattttcca
    15301 ggtacccgcg ttattcacta ccgcagagtt atagagaatc gcgcctttac gcgcctcgcc
    15361 gcctgcaagg ttattgaata ccgcatcgcc ctgagcgtta acgcgcgccg acgcaatacc
    15421 gctgttattc accgtaccat cgtgaagttg taaagttgac agggaaccgg tgttattaat
    15481 taccgcgttg ctgtttttct caatttttac gacgtcatta agcgttccgt tgttattgaa
    15541 ggttcctcct tccagagtca ccgtgttcaa tacaccttca ttggttaacg caccgccttc
    15601 attcacgacg atggctgagc taatggttcc gctcgactcg ttaaccagcg caccattttc
    15661 gagtatcctg atcgttccat ttttaacagc acccgcactg gtgacatccc cgttgataat
    15721 cgcacctgac gataataata cggtctcacc cgcagaactt aatacagcgc catcatcata
    15781 taaatcagca atataattat ccgttggatt atattcatcg gccccctagc aaacgccaaa
    15841 ggtacaaatt gtgccatagt tgataataag cccagtgcca tcgttataga atgcctgacc
    15901 attttccgcg ttaatgttga ttacgcctgt ctggtcatta atggcggtgc cttcgtgata
    15961 aaccgccatg ccgacgagtt tattcacacc gagagaatcg ttgtaatttt catttttctc
    16021 caggttgatg gttccctggt taatgaccgt attagtattc ccatacgccg ccatccccgc
    16081 accggcgttg tagacagtaa tcacaccatt ctcgtggttg gttgccgtgg ctttagttaa
    16141 tgccccatta taggcatcgg tacctgaagc cattcccaca ccgaaatctt tcgccgtgct
    16201 atttgcaaca ttattacgca gcgatgtttc atcattttca tcaacccaca gcgtatccag
    16261 ggtgatttgc ccttcgttat ccgcggttga tttatcgatg gctagcatcc ctacactgcc
    16321 acggccatac atatcaataa tacccgtttc gccgttcaga atggatgacg agtagctggc
    16381 aagcatacct gtcagcccta atgccttgtt attgaaagag gtaccatcag acaagttcca
    16441 ctctgccgga taattcagca cactgtgctg attcatcatt ttggtggtca tggtaccatt
    16501 gttaacgcca atcccaccgt attcaacatc aaagactttt ggtgccgggt cggtaccaaa
    16561 atcgtacatc gccaggccaa tggatccgtt attgattacc gttgaatctt tattccaggc
    16621 agccgcaaaa ccgatatttt tcagttccac ttcaccgttg tttgtcgcct tcgcaccaga
    16681 atcaatacta atggtggata acgctacacg ataataataa gtgaaagaat ccacatcact
    16741 catatcaaac gttgcgtcag cgccaatctc cagctccgcg ccatggctca actgaatgag
    16801 attttcgaac cccagcggga actcgccgct gatggttgac tcaccattca ggtagaccgg
    16861 ggaatgccca tcaacgttaa taagcggtgt gtctccgata ccacgtcgag aagctgatgg
    16921 ttttgagcca tcatccatga catttttttc accgacaaaa tggacaccgc cattaagcgt
    16981 aaaggtattg tcatcgccag caataacaat acctgcaatg tgttcctgag caccgtcgta
    17041 actcgcatag gaacgcgacg tggttttact atcagcaaca acggttaact gaccatccag
    17101 cgtgacgtcg ttgttgtcac cgctgatatt taccccggtt gagggattaa agaagtaagg
    17161 ctgcgcatta tcggctttct gatctttatc aaccagaata ctgcccgcta tttcgatagt
    17221 attaccatca cccgaaacat taacacccgt cgctttttgc cctgtcacat tcagctcata
    17281 agtcgccagc gtcatgttgt tattattgcc agagacatca agaccggtgg agaaatcccc
    17341 aacgtctaac ccacccgcga gtgaaatatt agctttatcc ccagcaacgc tgacaccggt
    17401 tccggcattg gtcgcggaaa tatcaccaac gttaacaaat gtcgcattat caccgtcgat
    17461 ctgaatgcca atggcatttt cacccgctgc ggcctgacca acattgaggt cgcccatgtt
    17521 gatcacgctg acgccatcac catcaagcag catgccaata gagttatctt cgatgaccgt
    17581 aatattacct ttgttgctaa cgaacgtatc attgccgtca acgtcaatac cgtgcgcacc
    17641 accgcttacg tataagtcgc cttcctgttt gatggtggca ccgtcaccga caattttcga
    17701 gccagtggaa ttctctccgt caacgctcat ggtaccttga ttatcgaccc gcgcattatc
    17761 gccattgata aggctacccg taccaccatt ggtgatggtc gagtcgcccg cagtgtttag
    17821 cttggtatta tcgccattga tgattaccgc ggtaccgcca tcaacttccg tctcgccatt
    17881 attgttaatg gtgatattgt cgccagtgag tataccgaca accgagcctt ctccgatggc
    17941 cgtcgttttg ccgtcgttgt tgagggagat gttgtcacca tcaaccgtca tgacagtacc
    18001 gccattcagc gccgtgttgc cgccctgatc ggtgatgatc gcgttaccaa actcaatcgc
    18061 attcccggtt gtgccgtcaa caacgacgcc ggcctcttta gtgatgtgga tgagtccatc
    18121 atcgtcgtaa ctccaggtga tgccacctga ggtatttacg ccatcaaaga caacggtgtt
    18181 gctgtcgtca tggactgtcc aggacttaac tatcgtctcc ttgccctcag gcgccgttaa
    18241 ggtgtagctc ccatcggcat tttggctgta ggtaaaggta gtttcgcgaa ttttgacagt
    18301 ttttgcatcc tgatcccaag ttacgccatt tttatagacg acaggattgt gtttaaccgg
    18361 ggtatcgcca ccatcgtctg gcggaactgg cgtatcaccg ccgtcatctg gcggaaccgg
    18421 cgtatcaccg ccgtcatcag gcggaactgg cgtatcaccg ccgtcgtctg gcggaacagg
    18481 cgtatcaccg ccatcatccg gaactggcgt atcaccgccg tcgtctggcg gaacaggcgt
    18541 atcaccgcca tcatccggaa ctggggagtt attatggtgg tgattgtcat cgtcacttaa
    18601 ggtatatata gcaattcccg tagctactgc agcaagacct gcagctgccc atggtaaaac
    18661 tgcactatct ttattttctc tgcactccgc ggataaattt tctagctgct ctttgctcag
    18721 ggaatgaata tttgccggac atgttatctt actggactta cgtgtttttt cggttgagtc
    18781 gggtattgaa gtatccgcag cccatgtctg gctacttaac gccatggcaa cacaaacaga
    18841 caataatttt tt
//
LOCUS       DBFIFM010000078        18674 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.078, whole genome shotgun sequence.
ACCESSION   DBFIFM010000078 DBFIFM010000000
VERSION     DBFIFM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.078"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..771)
                     /locus_tag="N7C74_002364"
     CDS             complement(<1..771)
                     /locus_tag="N7C74_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HGU2432225.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTKCHGESLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     MKEDNGNVSQTAKSPAERKRAQRERERLREQNTDCHDESRRVTHMSRQVTTDTDTDKE
                     LNPTHNARMRESAPASESNGAPLQTAEPEYPDGLSEPIGKFPMTGVWRPSLDFRQRAA
                     LWGMALPEPEFTPAELAAFRDYWMAEGKVFTQVQWEQKF"
     gene            complement(840..1265)
                     /locus_tag="N7C74_002365"
     CDS             complement(840..1265)
                     /locus_tag="N7C74_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU2432226.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAAAIAWGTLTNSGGQPGNAVVVH"
     gene            complement(1249..1572)
                     /locus_tag="N7C74_002366"
     CDS             complement(1249..1572)
                     /locus_tag="N7C74_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2432227.1"
                     /translation="MKKDNYSFKRACAVVGGQSAMARLLGVSPPSVNQWIKGVRQLPA
                     ERCPAIERATKGGVLCEELRPDVDWTYLRRSSCYSQNMSMKQPNDENDHTRSIKRQMI
                     HENQT"
     gene            1697..2173
                     /gene="racR"
                     /locus_tag="N7C74_002367"
                     /gene_synonym="rarC"
     CDS             1697..2173
                     /gene="racR"
                     /locus_tag="N7C74_002367"
                     /gene_synonym="rarC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor RacR"
                     /protein_id="HGU2432228.1"
                     /translation="MLSGKDLGRAIEQAINKKIASGAVKSKAEIARHFKVQPPSIHDW
                     IKKGSISKDKLPELWRFFSDVVGPEHWGLNEYPIPTPSTSDTKSELLDINSLYQAASD
                     EKRAIVAFLLSGNATEPSWVDHDVRAYIAAMEMKVANYLKNQESKRKSQNITKTGT"
     gene            2489..2641
                     /locus_tag="N7C74_002368"
     CDS             2489..2641
                     /locus_tag="N7C74_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU2432229.1"
                     /translation="MDTLDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARRW
                     LARNSD"
     gene            complement(2756..3271)
                     /locus_tag="N7C74_002369"
     CDS             complement(2756..3271)
                     /locus_tag="N7C74_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432230.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQRECVSLVELVGTYQEINASLKAKIAEFENFEAQTEGYILSQLESGTFVYSKEVTVN
                     GGSIIMHLCPKCFGQKIVSILQPFPVREYEFFHKSRCLYCENQFLMNKNPDYVSPPSI
                     EELARKLNGNL"
     gene            3395..3793
                     /locus_tag="N7C74_002370"
                     /pseudo
     CDS             3395..3793
                     /locus_tag="N7C74_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            3855..4124
                     /locus_tag="N7C74_002371"
     CDS             3855..4124
                     /locus_tag="N7C74_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU2432231.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            4093..5211
                     /locus_tag="N7C74_002372"
     CDS             4093..5211
                     /locus_tag="N7C74_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HGU2432232.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGVIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            5378..6172
                     /gene="potC"
                     /locus_tag="N7C74_002373"
     CDS             5378..6172
                     /gene="potC"
                     /locus_tag="N7C74_002373"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HGU2432233.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            6169..7215
                     /gene="potD"
                     /locus_tag="N7C74_002374"
     CDS             6169..7215
                     /gene="potD"
                     /locus_tag="N7C74_002374"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HGU2432234.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLSPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(7371..8192)
                     /gene="cobB"
                     /locus_tag="N7C74_002375"
     CDS             complement(7371..8192)
                     /gene="cobB"
                     /locus_tag="N7C74_002375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HGU2432235.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(8208..9119)
                     /gene="nagK"
                     /locus_tag="N7C74_002376"
     CDS             complement(8208..9119)
                     /gene="nagK"
                     /locus_tag="N7C74_002376"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HGU2432236.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLPAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(9148..10392)
                     /gene="lolE"
                     /locus_tag="N7C74_002377"
     CDS             complement(9148..10392)
                     /gene="lolE"
                     /locus_tag="N7C74_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HGU2432237.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(10392..11093)
                     /gene="lolD"
                     /locus_tag="N7C74_002378"
     CDS             complement(10392..11093)
                     /gene="lolD"
                     /locus_tag="N7C74_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HGU2432238.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPWLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(11086..12285)
                     /gene="lolC"
                     /locus_tag="N7C74_002379"
     CDS             complement(11086..12285)
                     /gene="lolC"
                     /locus_tag="N7C74_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HGU2432239.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            12547..13620
                     /gene="ycfT"
                     /locus_tag="N7C74_002380"
     CDS             12547..13620
                     /gene="ycfT"
                     /locus_tag="N7C74_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HGU2432240.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLAALAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPVAVSYSR"
     gene            13748..17194
                     /gene="mfd"
                     /locus_tag="N7C74_002381"
     CDS             13748..17194
                     /gene="mfd"
                     /locus_tag="N7C74_002381"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HGU2432241.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMEKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     LSRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            17338..18300
                     /gene="ldtC"
                     /locus_tag="N7C74_002382"
     CDS             17338..18300
                     /gene="ldtC"
                     /locus_tag="N7C74_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HGU2432242.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(18383..18640)
                     /gene="bhsA"
                     /locus_tag="N7C74_002383"
                     /gene_synonym="ycfR"
     CDS             complement(18383..18640)
                     /gene="bhsA"
                     /locus_tag="N7C74_002383"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HGU2432243.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         4722 a         4843 c         4638 g         4471 t
ORIGIN      
        1 aaatttctgc tcccactgaa cctgcgtgaa aaccttcccc tccgccatcc agtaatcccg
       61 gaatgcggca agctcagcag gtgtaaactc aggctcaggc agagccatac cccacaatgc
      121 agcccgctgt cgaaaatcca gcgacggccg ccagacacca gtcatcggaa atttcccgat
      181 cggttcgctc aggccatccg ggtattcagg ttctgctgtc tgcaacggcg cgccattcga
      241 ctcactggct ggagcactct cgcgcatgcg cgcgttatgt gtggggttta attctttatc
      301 tgtatctgta tctgtcgtga cttgtcgtga catatgcgtg acgcgtcgtg actcatcgtg
      361 acaatcagtg ttctgctccc gcagtctttc ccgctcccgc tgcgctctct tgcgctctgc
      421 cggggatttt gcggtttgcg aaacattacc gttatcctcc ttcatcacct ggcgtttttc
      481 ccatccggaa ataagatcgc catccagaac tcgcccctgc attgcatgca aaattgaatc
      541 aatcacgtct tccgtcacat caagcgcact tgctaaatct tccgtcgtga catcaatgtg
      601 accacgtagt gactcgccgt gacatttcgt gacatttcgt gacgcactca ccagaaggtg
      661 gatatacacc gccatcactg ttgcgattgg ctgtcctgag accctggcaa tggttcgcca
      721 tttgggatca tttggcatgt catgccacaa tctgagccag gcattagcca tactcacctc
      781 atctgatacc gaactttacc ctcgaacatc cggaagaaat ccggcatgaa tattgttggt
      841 caatgcacga caacagcatt accaggctga cccccactgt tagtcagggt gccccaggcg
      901 atcgccgctg cgacaaaatc atccacatct ttcaccagcc gatccctccg ttcgacgatc
      961 tcacggtaat attcagagct gtgactgcgc atacgggcca ccagcagagg cggcattgcc
     1021 ttttcgatcg ccggtaacag agcctgcatt ttttcaacag catcaggggt gtctttatcc
     1081 agccaacgga aaattttctg ggtattacga gccagggctt ccggatggct gtcgtcgtac
     1141 agttccggga acgtcatccc cagttcgaaa taagtccggg ctatttcagc tgcaggaact
     1201 ttctcaccat caggatatgc ccaggcattc atcgccatgc ggatgtgctc atgtttgatt
     1261 ttcatgaatc atttgcctct tgatgcttcg ggtatgatcg ttttcgtcat ttggttgctt
     1321 catcgacata ttctgcgaat aacatgacga gcgtcgtaag tatgtccaat caacatcagg
     1381 acgaagttct tcacacagga caccaccttt tgttgctcgt tcaatcgcag gacatctctc
     1441 agcaggcaac tgacgtacac ctttgatcca ttgatttacg cttggaggag atacacctaa
     1501 aagcctagcc attgctgatt gcccaccgac aacagcacaa gctcgtttga atgaatagtt
     1561 atcttttttc atcgaatgaa ctccaaaaaa cacacaataa tattaggctt agcctaatgc
     1621 aactgtcaat aggctatgcc taacgcttcg agagtaggta ttgcctaatg cgatgcgcat
     1681 aggagactat taagcaatgc ttagtggtaa agacttaggc cgagcgatag agcaggccat
     1741 taacaaaaaa attgcatcag gagccgtcaa atcaaaggcg gaaatcgcac gtcatttcaa
     1801 agtccaacca ccatcaatcc atgactggat taagaaaggt tcgataagta aagacaaact
     1861 tccagaacta tggcgtttct tttctgatgt ggttggtcca gagcattggg ggcttaacga
     1921 ataccccata ccaaccccat ccacttcaga tacaaaaagt gaacttttag acataaacag
     1981 cctttatcaa gccgcctctg atgaaaaaag agcaattgtg gctttcctct tatctggaaa
     2041 tgctacggag cctagttggg ttgatcatga cgttcgtgcc tacattgccg caatggaaat
     2101 gaaggtagct aactatctga aaaatcaaga atcaaaacgg aaaagccaga acatcaccaa
     2161 gacaggaact taaacttata tggtccgacg ggaaattcct agttcccgtt agttaactcc
     2221 tactacctct cccacaaacc atcacctatt aggtcgcacc caaatcatta ggcgtagcct
     2281 attgacaagc aattaggcat ttcctatagt tttcccatac caacccatcc cgtcccacac
     2341 aatacagggc aatacctaga gttacccggc agtggtcagg gattaagtag ccagcccgag
     2401 gcgtaagaac atgacggcag ggttcaactt taactatgca gcaggttttt gttccgctac
     2461 cccggcgtta aggggatatg aggtcagcat ggatactctt gatcttggca acaacgaatc
     2521 tctggtgtgc ggcgtgtttc ccaaccagga cggcacgttc accgcgatga cgtataccaa
     2581 aagcaaaacg tttaaaaccg aatctggcgc gcgtcgctgg ttagcaagaa actctgacta
     2641 atgaaatctg gtaattaagg aatcctccac ggggggagag tggtgcacac gcgccggaca
     2701 caagcaagca tccggcatgc tctttaacaa tctggatatc cataacagta aaaatctaca
     2761 gattgccgtt cagttttctg gccaactctt caatagatgg aggtgatacg taatccggat
     2821 ttttattcat aagaaactga ttttcacagt acaggcacct gcttttatga aaaaattcat
     2881 attctctaac cgggaatggc tgaagtatcg atactatctt ttgtccaaaa cattttggac
     2941 aaagatgcat gattatgctg ccgccgttca cggttacctc cttcgagtac acaaaagttc
     3001 ccgactcaag ttggctaagg atataacctt ccgtctgagc ctcaaagttt tcaaattctg
     3061 caattttagc tttgagagaa gcatttattt cttgataagt gcccaccagc tcaacgagag
     3121 acacgcattc gcgctgaata gatgcaagct ttgagttcag ttcaccaata gccgcattta
     3181 cttcagcttg agtttttgcc tcgttcatta gttttgcaat ctgggcggtt tcacgaatag
     3241 ccgtcattgc tgccgttaat tcagcgatca cattcaatac tcttattgtt gttggggata
     3301 tccagattaa caagatcctt gttgttgggg aataacgggg accacctcgc ctgacgtggt
     3361 aaaaagcagg cacacaacac gaaagcacac ggcgagggac accattaaat ggtggcttaa
     3421 gcaatcacgc gaagcgcaat ctgccattat gaccgatgaa atcccgttag atgatgcact
     3481 gttacaattg cgggaattta tcgacgaaaa ctccggtgaa ttttttgttc aggtctgggg
     3541 aaatggagcc aacttcgaca acacgatttt gcgccgttca tacgaacggc aggggatccc
     3601 ctgcccgtgg cgttactaca acgatcgcga tgtacgcaca atcgttgagc tggggaaagc
     3661 catagacttc gatgccagaa cggctattcc attcgaaggt gagcgccata atgcacttga
     3721 tgacgcccgt taccaggcaa aatacgtttc agttatctgg caaaaactga tcccgagtca
     3781 ggctgatttt taatgttcaa ccgtcgccag ttgtcgttga tattctgcaa ctggcgcgtt
     3841 ccggagtgat agccatgagc gaacagtacc tgataacgct cgacgagtgg aaaccaaaac
     3901 ggttcagtct cccaataaca aacactaccc tggtgaaata cggaaaacta ggatacatcg
     3961 ttccaagacc acaaaaaatt cgtgggcgtt ggctgataga tcgccgagca gtatttgttg
     4021 ggcctggtga aacgggaatt gcgccggaaa ttcatactgg cgatgatgat gcactgaagg
     4081 agattttaac tcatgtcacc gaggccacga aaaaacagca ctgacgtagc cggtctttac
     4141 gaaaagtttg atcgcagaac tggcagagtt tactaccagt ataaaaatcc tgtgactgga
     4201 aaatttcacg gactcggaac agacaaaggt aaggcagaaa aaatcgcttc cacagccaat
     4261 cagcgaatag ctgcagcaga agctgaatat ttcatgcgca aaattgatga aagtccgtca
     4321 gcaacaaaac gtcggggtat cagattaaag gcatgggttg atcgatatct gaaaatacag
     4381 gacacgcgac tgaaaaatgg agatattgca gctacaactc acaaagaaaa aactcgaatg
     4441 gctgcatacc tggtttcccg tctgggaaac cacccattga aagaactgga agtaagagac
     4501 tttgcattaa tactggatga gtggctggat aaagacatgg tcagtacagc gagagtaaat
     4561 cgtggattat gggttgatat ttataaagaa gcacagcatg caggggaagt tcctcctgga
     4621 tggaatcctc cggaggctac ccgtaaaccg atccctaaag taaccagagc caggctcacc
     4681 atggaagact ggcaaaaaat ttacaatgca acgcctgaaa aacactttat ccgtaacgca
     4741 atgcttcttg cgattgttac tggtcagcgc cgtgatgaca tttgccacat gcgtttttca
     4801 gatgtgtgga acgaacactt gcatatcacc cagggaaaaa ccggaatgcg tctggcgtta
     4861 ccgcttacac tacgctgtga tgccattggg ataacgttaa aagaagttat tgatgggtgc
     4921 cgagacagaa tattaagtcc atatctaatc catagtcggc accagaaaca accgaagccg
     4981 atgagtaaag acaacctgag cgactacttt gccaaagcac gggatctggc tggggtaatt
     5041 ccaccagcag gaaaaactcc gccaacattt catgaacaac gctctctatc agaacggctg
     5101 taccgtgcac agggtatcga tacaaaaaca ttactaggac ataaagtcca ggcaaccacc
     5161 gatcgctata acgatactcg aggtcaggaa tgggttaagt tggttatttg acaaacacca
     5221 tatggaatat ccgtcaccat ctcaggaaaa aagtgttgta aaatgcgtgg cctagttttg
     5281 gggaaacgtt ttggagagat tttggagaaa ggaaaaaatt gaataaattc aaaatcctga
     5341 aagtgtcccg ccttatgaat aaaaaggtgg aactcgaatg atcggtcgac tgcttcgcgg
     5401 cggttttatg accgctatct acgcgtacct gtatatccca atcattattt tgattgtgaa
     5461 ctcctttaac agctcgcgct ttggcatcaa ctggcagggt tttaccacca aatggtatag
     5521 cctgctgatg aacaacgaca gcctgttaca ggcagcgcag cattcactaa caatggcggt
     5581 gttttcggcg acgtttgcta cgcttatcgg ttcactgacg gcagttgcgc tgtaccgtta
     5641 tcgctttcgt ggtaagccgt tcgttagcgg aatgctgttt gtggtgatga tgtcaccaga
     5701 tatcgtgatg gcgatttcgc tgctggtgct gtttatgctg ctgggtattc agcttggctt
     5761 ctggtcgctg ctgttctcgc atatcacctt ctgcctgcca tttgtggtgg tgacggtgta
     5821 ttcgcgcctg aaaggttttg acgtgcggat gctggaagcg gcgaaagatc tcggtgccag
     5881 cgaatttacc attctgcgaa aaatcattct gccactggca atgccagcgg tggcggcagg
     5941 ctgggtgtta agctttaccc tgtcgatgga cgatgtggtg gtttcttcgt tcgtcaccgg
     6001 gccgagttat gaaattctgc cgttaaaaat ttattcgatg gtcaaagtcg gcgtatcgcc
     6061 ggaagttaac gcgctggcaa ccatattact ggtgctgtcg ctggtgatgg ttattgccag
     6121 ccagcttatt gctcgtgata aaacgaaagg taacacaggg gacgttaaat gaaaaaatgg
     6181 tcacgccacc tgctcgcggc gggtgctctg gcactgggca tgagcgccgc tcacgccgat
     6241 gacaacaaca cgctgtattt ctacaactgg accgagtacg tgccgccagg actgcttgaa
     6301 cagttcacca aagaaaccgg tattaaggtt atctattcga cttacgagtc gaacgaaacc
     6361 atgtatgcca agctgaaaac gtacaaagac ggtgcctatg acctggtggt tccttcaacc
     6421 tattacgtcg ataaaatgcg taaagaaggg atgatccaga agatcgacaa gtcgaagtta
     6481 acgaacttca gcaatctcga tccagacatg ctcaataagc cgtttgaccc gaataacgat
     6541 tactccattc cgtatatctg gggtgcgacg gcgattggcg ttaacggtga tgcggtggat
     6601 ccgaaatctg tcaccagctg ggccgatttg tggaagcctg agtacaaagg cagcctgctg
     6661 ctgaccgacg atgcccgtga agtgttccag atggcgctac gtaagctggg ctactccggt
     6721 aacaccaccg atccgaaaga gattgaagct gcatataacg agctgaaaaa actgatgcca
     6781 aacgtcgcag cgtttaactc cgataacccg gctaacccgt acatggaagg cgaagttaac
     6841 ctcggcatga tctggaacgg ttctgctttc gttgcacgcc aggcgggtac gcctattgac
     6901 gtggtgtggc cgaaagaagg cggcatattc tggatggaca gcctggcgat cccggcaaat
     6961 gccaaaaaca aagaaggcgc gctgaaattg atcaacttcc tgctgcgccc ggatgtggca
     7021 aaacaggttg ctgaaactat cggttatcca acgccaaacc ttgcggcgcg taagctgtta
     7081 agtccagaag tggcgaacga taagacgctc tccccggatg ctgaaaccat taaaaatggc
     7141 gaatggcaga atgacgttgg cgcagccagc agcatttatg aagagtatta tcagaagctg
     7201 aaagcaggac gttaattcag caagcgggcg gtggtattac cgcccgtttt ttagttagca
     7261 aggatgtggc taatgagtca ggacagtaaa gttttatccc gctctttcgt cggtattgtg
     7321 gtccgcttgt aggcctgata agcgtagcgc atcaggcaat gcttcctgct ttataatccc
     7381 ttcagcaact tttcaacaaa ttctggcacc acctggcttg ccgggccgta atatttctcg
     7441 gcaaattcat taccgacctg acttggttca agattcagct ccacggtgtg cgcgccatgc
     7501 agtttcgctt cgtgaacaaa cccagccgcc ggataaacat gcccggaagt accaatggca
     7561 atgaaaatat cggccatcga caacgccata taaatttcat ccatgccgag tggcatttcg
     7621 ccaaaccata ctacgtgtgg gcgcaagggg gccgggaact ggcagcaatg gcatttatct
     7681 tctggggtaa cgtctccggt ccagtcgaga acctgaccac tttgtgaaca acgaactttc
     7741 agcagttccc catgcatatg aatcacattg gtattacctg cgcgttcatg caggttgtct
     7801 atattctgcg tcaccagcaa aaagcgatcg ccgagagcat cttgcagttt agccagcgca
     7861 agatgcgcgg cgttaggctg aatttctggc tgctgcagct gtcgacgacg ggcgttataa
     7921 aacgcttgca ccagttcagg atcgcgatcg aaaccttccg gcgttgccac atcttcaacc
     7981 cgatgttctt cccacaggcc atctgcggcg cgaaaggtac gaatacctga ttccgcagaa
     8041 attcctgccc ctgtcagtac gagtactctt ggtttttcca ttgcttccgg caccacttta
     8101 tctctgaaaa aaatacgctg acgcaaacgc tcgcgcaggc ggcgtttatt tttacgaaaa
     8161 cgacttaacc gatgaccccg acgcgacagc atagcaacct ctgttgttta atcggttaga
     8221 tgtaggaagg ccgcaccacg cattcctccc gcatcaccgt ggcgcgcgcg ttcaatgcgc
     8281 ggaacacgag ctacaggtaa gagatgacga ggcagcctgt ccgccagttg cgttgtgatt
     8341 gccgggaaat tcgataagcc accaccaatg acgaccaggt cagggtcaac aatggtcagg
     8401 atatttccca gacaaaccgc taataaatcc agataacgct caacgtgcgc ccttgcctgc
     8461 tcatcgcctt gatcataaag cgcaataatt tcgggagccg gcaacggttg atgataatag
     8521 tgttgataca gccacgcaaa accgcgacca gacagataat tttcaatgca gccatgctga
     8581 ccacagccgc agcggcgtaa cgggaaatcc agccccatca tggttaacgc atcaaccggc
     8641 agacgcatat ggccaaactc gccggtaatg tagcttttcc cggtaatcgg tttgccgttg
     8701 aaaatcagcc cgccgccaac gccggtgccg agaatcaacc ccatcaccaa cggatattgc
     8761 gtaaattcgt catcccaggc ttctgaaagg gcaaaacagt tggcatcgtt atcaaggcgt
     8821 acatcgcgat caagacgtgc gctcaggtcg gcacgcagcg gtttaccgct ggcagcaggg
     8881 acattggcgg catacagcgt accatcttct gtttccggca ttcccggaat accgatgccg
     8941 acagagcctt tacagccaaa acgttgatca gcttcggcta ccagctcgca cactgcatct
     9001 aaaaatgcgt catagctgtc acgcggtgtc ggcacccgct tttcccactg caactgccga
     9061 ccgctatcaa acacgccaag cgcaattttt gttccaccaa tatcaaaccc gtaatacatt
     9121 gccgctcctt gttttaatgt actgccttta ctggccgcta aggactcgcg cagggtcaat
     9181 attactggcg cgccgcgccg gataccaact tgccaaaaga ctcagcaaca atgctgtgac
     9241 cagtacgtag aagacgtcca gccagtgcaa ttccgatggc aggaagtcaa taaaatagat
     9301 atcgctggag aggaactgat gaccgatcag cttttcaatc cgctcaataa tcggggtaag
     9361 ttgcagtgaa acaactacgc cgataatcac accacacagg ctaccgaata gccctgccag
     9421 caatccatac cagacaaaga tggcgcgaat taaaccatct ttcgccccca gcgttcttaa
     9481 tactgcgata tcgccactct tgtctttcac cgccatcact aaggtggaga cgatgttgaa
     9541 acaggccacg ccaatcacca gtaccatcgc cagatacata atggcgcgga tcatctggat
     9601 atcgcgatac atatagccgt aagtaccaat ccagctttta atataaacat agctgttggt
     9661 cacttcaccc gcatcgcgta ccagcttatt ggcgttgaaa acatccgtca ttttaagggc
     9721 aatacctgac acgctggaac ccatatcaag atattgttgg gcatccgcca gcgggatcat
     9781 ggcaaaactg tgatcgagtt gaccactcaa ctgcaaaata ccggcaacgt gcaaacgcac
     9841 acgttttggc tgcatcagtt tatgctcagg attcgagttg gggatcataa tcgacaccca
     9901 atcgccctgc ttcactttca gcgcatccgc cacgcctttg ccgatgataa tttgctgttc
     9961 gcccgcttta aaattgcgcc aggcatcccc ctgaacaaac gagggtaatg cgctcagacg
    10021 ctgttcctgt tgcgggttaa cgcccttcac ctggattgcg cgcagattcg ctccactttc
    10081 caccagcccg gtgaaattga tatacggcgc agcggcggca atacctggca ctttttgcac
    10141 gttatccagt gcttcctgcc agttagtcca cggttgatcc accgcctcta tttcgccatg
    10201 cggcaccacc gccagaatgc ggttattcag ttcgcgttca aagccgttca tcgcgcttaa
    10261 gccgacgatc aataccgcca cgccaagggc aatgccaatg gtagaaatga cggagatcag
    10321 cgacaccatg ccgccgcgtc gccgtccgcg gctaaaacgc aggccaatca ataacgataa
    10381 aggcatcgcc attactccgc ccccatcagg ctcagttccg ccgtcagacg accatcacgc
    10441 atctccagtt ggcggctcat acgtttcgcc agttgcaggt cgtgagtaac caccaggaag
    10501 gcggtgccct gcaagcgatt caattcccca agcaactgga agatgctgtc tgcgttacgc
    10561 gcatcgaggt taccggtagg ttcatccgcc agtaccagcc acgggttatt gaccagcgca
    10621 cgggcaatcg ccacacgctg gcgttcgccg ccagaaagtt cagatgggcg gtgattcgca
    10681 cgatgctcca gccccaccgc ttttaacatc tcaagtgcac ggctgttgat ttcagcgggc
    10741 tttttcttgc caatcagcag cggcatagcc acgttttcca gggcagtaaa atccggcagc
    10801 aggtggtgaa actgataaat aaagcccagc ttctggttgc gcagttctgc tttcgccgcc
    10861 gaagacagtt tgctcatcgg ttgaccgtta aagatcacat cgccggaggt tggtgtatcc
    10921 agcccgccca gcaggtgcag caaggtactt ttaccggaac cagagctacc gacgatcgcc
    10981 atcatttcac cttcgcccac gctgaaactg acattgtgca gtacatcggt ttgcacactg
    11041 ccttcctgat agcgtttgca caggttgtcg cattgcaaca ggatcttatt cataacgtaa
    11101 agcctcagcg ggttgtgtgg cggcagcgcg ccatgaaggg taaagcgtag acagcagcgc
    11161 gatagccatc gccaccagcg caataacaat gacctgtaaa ggttcgatag ccaccggcag
    11221 cgccgcgcca tcaagcagga cgccgattat cggcatcaga ttatttaact ggctggcaag
    11281 cagtgcgcca agcgccgctc cgaggatcgc accgataatc ccggcgctgg ccccttgcac
    11341 cataaagacc atcatgattt gtcgcggagt taagccttgc gtttgcagga tcgctacttc
    11401 gccctgcttc tccatcacca tcagccccag cgaggtaata atgttaaacg ccgcaacggc
    11461 gacaatcagg ctcagcagta agcccatcat atttttttcc atgcgtacgg cctggaacag
    11521 ctcgccttta cgatcacgcc agtcctgcca tttgctgcct tcaggcagtt tttgctgact
    11581 taaagagtca actttcagcg gctcatccag ccacaaacgc cagccggtaa tattgcctgc
    11641 cggataacgc atcagacgcg aggcatcctc aatattcacc agcatttcat agccatcgac
    11701 ttcactgtta gcggcaaaag taccaatcac attgaacagg cgctgacttg gaatacgccc
    11761 catcggcgtg aactggctgg cagatggcac catcacgcgg atttgatcac cgcgattaac
    11821 gcctagctgt gaggcaagtt gttcgccgag gatgacatta tatttccccg gctcgaggtc
    11881 agtttgtttc acattgacca gatacggcgt tagtggatct ttttgcgccg gatcgatacc
    11941 cagcatcacc ccgaccgcca cgctgcgcgc gctttgcagt accacatcac cggtagtaat
    12001 aggtgcgacg cgattaacgc cgtccagttt gactgccgtt tccgggagtt gctgcgggtt
    12061 aagagagcca tgctcagaag agagaattgc ctgtggcatc aggccaagga tgttgttttg
    12121 cagctcgcgc tcaaagccgt tcatcactga caatactgtg accagcgcca tcaccccgag
    12181 ggtaatgccg atggtagaaa gccaggagac gaaacgaccg aagcgatccg ctgcacgccc
    12241 acgcatgtaa cgcaggccaa taaatagagc gacaggttgg tacatgaaat ccgtctggtt
    12301 gctgtagcaa agacacgagt atataagcga aagcaagatg ggtaaacgtc tgaactgtaa
    12361 gtgatcgtca gggctaatct gaaaagcccc cgtaaatcaa tcggctaaat tgccatgttt
    12421 tatcatcaat cttgccgcct gatcttaatc tgaaaaatca tccaatacag acagataccc
    12481 attacacctc gggtcataac ccgcaggatc aaccgcatgt aaattaaaaa agagcgtgtt
    12541 gtcctgatga aacaaaaaga gctatggatt aaccagatca aagggttatg tatttgtctg
    12601 gtggtgattt atcactcggt cattaccttt tatccgcata tgaccacttt tcagcatccg
    12661 ttatcggaag tcctgagcaa atgctggatc tatttcaatc tttaccttgc accctttcgt
    12721 atgccggttt ttttctttat atccggctat ttgattcgcc gctatatcga cagcgtaccg
    12781 tggggaaatt gtctcgataa acggatctgg aacatcttct gggtgctggc gctttggggc
    12841 gtggtgcagt ggctggcgtt aagtgcgctg aaccagtggc tggcacctga gcgcgattta
    12901 agcaatgcct ccaatgccgc gtatgccgat tccaccggtg agttcctgca cgggatgatc
    12961 accgccagca ccagcttgtg gtatctgtat gcgttaattg tctatttcgt ggtatgtaaa
    13021 atttttagcc gtctggcgct gccactattc gccttgtttg tactgctgag tgtggcggtt
    13081 aatttcgttc ccacgccgtg gtggggaatg aacagtgtga tccgcaattt gccttattac
    13141 agccttggtg catggtttgg cgcaacaata atgacctgtg ttaaagaggt gccgttgcgc
    13201 cgccatctgc tgatggcttc tttgctggct gctctggcgg tcggtgcctg gttgtttact
    13261 atctcgctgc tgttgtcgct ggtgtcgatt gtggtgatca tgaagttgtt ttatcagtat
    13321 gaacaacgtt ttggcatgcg ttccaccagt ctgctgaatg tgattggctc caacactatt
    13381 gctatctaca ctacccatag aattctggta gagatattca gcttaactct gcttgcgcaa
    13441 atgaacgcag cacgctggtc gccgcaagtc gaactgacac tcctgctggt ttaccccttt
    13501 gttagtttgt tcatctgtac tgttgcgggc ttgctggtaa gaaaactttc acagcgagca
    13561 ttcagcgatc tgttgttctc cccgccttca ctgcccgttg ccgtcagtta ctcccgctaa
    13621 gccatcgccc cagccggggc gattcaattt gctaaaccat gtcgattacg gataatgaac
    13681 agcattgctt atcagagata tgcccccata tgttgaggca tatcctaacg agaatctgac
    13741 aaccgttatg cctgaacaat atcgttatac gctgcccgtc aaagcgggtg agcagcgtct
    13801 gctgggcgag ttaaccggcg ctgcctgcgc gacgctggta gcggaaattg ccgaacgtca
    13861 cgccggaccg gtggtgctta ttgcgccaga tatgcaaaat gccctgcgtt tgcatgatga
    13921 aattagccag tttaccgacc agatggtgat gaatctggcg gactgggaaa ccctccctta
    13981 cgacagtttt tcgcctcatc aggacattat ctcctcgcgc ctttccaccc tttaccagct
    14041 accgacaatg cagcgtggcg tactgattgt tccggtgaat acacttatgc agcgcgtttg
    14101 cccgcacagt tttctccacg gtcatgcgct ggtgatggaa aaaggtcagc gcctgtcacg
    14161 agatgcatta cgaacccaac tggatagcgc cggttatcgc catgttgacc aggtgatgga
    14221 gcacggcgaa tacgccacgc gcggcgcgtt gctggatctc ttcccgatgg ggagtgagtt
    14281 gccttatcgt cttgatttct ttgatgatga aatcgacagt ctgcgggtgt ttgacgtcga
    14341 cagtcagcgc acgctggagg aagtagaagc gatcaacctg ctgcctgcgc acgaatttcc
    14401 gaccgataaa gcggcgattg aactgttccg cagccagtgg cgcgatacct tcgaagtgaa
    14461 gcgcgatccg gaacatattt accagcaagt gagtaaaggc acattacctg ccgggatcga
    14521 gtactggcag ccattgttct tcagcgaacc actgccgccg ctgttcagtt atttccctgc
    14581 caataccttg ctggtgaata ctggcgatct ggaaaacagt gccgaacgtt tccaggctga
    14641 cacgctggcg cgttttgaga atcgcggcgt cgatccgatg cgcccactgt tgccaccaca
    14701 atcgctctgg ctgcgggtgg acgagctctt ctcagagctg aaaaactggc cccgagtgca
    14761 gctaaaaact gaacatttac cgacaaaagc cgcgaatgcc aatttaggtt tccagaaact
    14821 gccagacctg gccgttcagg cacaacaaaa agcgccgctg gatgcgctgc gtaagttcct
    14881 cgagtctttc gacggtccgg tggtgttctc ggtagaaagt gaaggccgcc gtgaagcgct
    14941 gggtgaactg ctcgcgcgaa ttaaaattgc tccgcaacgc attatgcgtc ttgatgaagc
    15001 cagcgaccgt gggcgttatc tgatgattgg cgctgcagag catggttttg tcgatacggt
    15061 acgtaatctg gcgctgattt gcgaaagcga tctgctcggt gaacgcgttg cccgtcgtcg
    15121 tcaggattct cgccgcacca tcaaccccga tacactgatc cgtaacctcg cggaactgca
    15181 tattggtcag ccggtggtcc atctggagca cggcgtcggg cgctatgccg gaatgaccac
    15241 gctggaagca ggcggcatta ccggcgagta tctgatgctc acctatgcca acgacgccaa
    15301 attgtatgtt ccggtgtcgt cgctgcatct gattagccgt tacgcgggtg gtgcggaaga
    15361 aaatgccccg ctgcataaac ttggcggcga tgcgtggtca cgcgcgcggc agaaagcggc
    15421 ggaaaaagtg cgtgatgtgg cggcggaatt gctggatatc tacgcgcaac gcgccgccaa
    15481 agagggcttc gcgtttaaac acgatcgtga gcagtatcag ttgttctgcg acagcttccc
    15541 gtttgaaacc acgccggatc aggcgcaggc cattaatgcg gtacttagcg acatgtgtca
    15601 gccgctggca atggatcgtc tggtgtgcgg cgatgttggc tttggtaaaa cagaagtggc
    15661 gatgcgcgcc gctttcctgg cagtagataa ccacaagcag gtggcggtgc tggtgcctac
    15721 cacccttctc gcgcagcagc attacgacaa cttccgcgac cgtttcgcca actggccggt
    15781 acgtatcgaa atgctctccc gtttccgcag cgccaaagag cagacgcaaa tccttgcgga
    15841 agtggcggaa gggaaaatcg atattctgat cggtacgcac aaactgctgc aaagtgacgt
    15901 caagtttaaa gatttaggcc tgctgattgt cgatgaagaa caccgcttcg gggtgcgtca
    15961 taaagagcgc attaaagcga tgcgcgcgaa cgtggatatt ctgacgctta ctgcaacgcc
    16021 gatcccacgt acgctgaata tggcaatgag cggaatgcgt gacctatcga ttatcgccac
    16081 gccgcccgcc cgtcgtctgg cagttaaaac ctttgtccgt gagtatgaca gcctggtggt
    16141 ccgcgaggcg atcctgcgtg aaattttgcg cggaggacag gtttattatc tctacaatga
    16201 tgtggaaaac attcagaaag ccgccgaacg gctggcagaa ctggtgccag aagcgcggat
    16261 cgccatcggt cacgggcaga tgcgcgagcg cgaactggaa cgggtgatga atgatttcca
    16321 tcatcaacgt ttcaacgtgc tggtttgtac aaccattatc gaaaccggga tcgacatccc
    16381 gacagccaac accattatca ttgaacgcgc ggatcacttc ggtctggcgc aactgcacca
    16441 gttacgcggt cgcgtcggac gttcgcatca tcaggcatat gcatggctgc tgacgccgca
    16501 tccaaaagcg atgactaccg atgcacaaaa acgtcttgaa gcgattgcct cgctggaaga
    16561 tctcggggca ggttttgcgc tggcaacgca cgatctggag attcgcggcg cgggtgaact
    16621 gcttggcgaa gaacaaagcg gctcaatgga aaccatcggt ttctcgctgt atatggagtt
    16681 gctggaaaac gccgtcgatg cactgaaagc cggacgcgag ccgtcgctgg aagatctcac
    16741 cagccagcaa acagaagtcg agctgcggat gccgtcgcta ttgccagatg atttcattcc
    16801 tgacgtgaat acgcgtctgt cgttctataa acgtattgcc agcgccaaaa cggaaaacga
    16861 actggaagag atcaaagtcg agcttatcga tcgcttcggc ctgctgccgg atccggcgcg
    16921 taccctgctg gatattgccc gtctacgcca gcaagcgcag aaactgggga tcaggaagct
    16981 ggaaggtaat gagaaaggcg gcgtgatcga atttgccgag aagaatcacg ttaatccggc
    17041 ctggttgatt ggtttgctgc aaaaacagcc gcagcattac cgccttgatg gtccgacgcg
    17101 cctgaaattt attcaggatt tgagtgagcg gaaaacgcgt atcgaatggg tacgccagtt
    17161 tatgcgtgaa ctggaagaaa acgcgatcgc ttaatggtaa atcgggggcg tttctgcgcc
    17221 cccatctgca acatttacaa attctttgca cttccctgca ctatccgaca ctgtcaccat
    17281 ccataattca ggcttatctg tttattacaa taaccttata tttattatgg atttttggtg
    17341 atgatcaaaa cgcgtttttc tcgctggcta acgtttttta cgttcgccgc tgccgtggcg
    17401 ctggcgctac cggcaaaagc caacacctgg ccgctgccgc cagcgggcag tcgtctggtt
    17461 ggcgaaaaca aatttcatgt ggtggaaaat gacggtggtt ctctggaagc catcgccaaa
    17521 aaatataacg tcggctttct cgctctatta caggctaacc ccggcgttga tccttacgta
    17581 ccgcgcgcgg gcagcgtgtt aacgatcccg ttgcaaaccc tacttccaga cgcgccgcgc
    17641 gaaggcattg tgatcaacat tgcggagctt cgtctctatt actacccgcc gggtaaaaat
    17701 tcggtaaccg tgtatccaat aggtattggt cagttaggtg gtgacacgct gacaccgaca
    17761 atggtgacca ccgtttctga caagcgtgca aacccaacct ggacgccaac ggcaaacatt
    17821 cgcgcgcggt ataaggcaca aggaattgag ttgcctgcgg tagtgccggc tggaccggat
    17881 aatccaatgg gccatcatgc gattcgtctg gcggcctatg gcggcgttta tttgcttcac
    17941 ggtacgaacg ccgatttcgg cattggcatg cgggtaagtt ctggctgtat tcgtctgcgg
    18001 gatgacgata tcaaaacact ctttagccag gtcaccccag gcaccaaagt gaatatcatc
    18061 aacactccga taaaagtctc tgccgaacca aacggtgcgc gtctggttga agtacatcag
    18121 ccgctgtcag agaagattga tgacgatccg cagctgctgc caattacgct gaatagcgca
    18181 atgcaatcat ttaaagatgc agcacaaact gacgctgaag tgatgcaaca tgtgatggat
    18241 gtccgttccg ggatgccggt ggatgtccgc cgtcatcaag tgagcccaca aacgctgtaa
    18301 atcctggata aaaaaaatcc ccgcgacacg gcatgtcgcg gggaaaaaat cagcagtagc
    18361 aggcattaat gagggttaat gcttatttat aaattactgc tgttccatgg agggtattcg
    18421 gaccggttac agaagtaata cggaaagatt ttgcgcccat ctcatccgct ttttgcgcca
    18481 gctgctcttc cagcgatccc agatttgtcc ccgcgttagc actgattgta ccgacttttt
    18541 gttggccttc tggcgttgac tgaacttcga cagccgcaaa gctggcaaat gacatggagc
    18601 ttaaaatcgc cgcagcgatg agggttttta cgtttttcat aatagtggcc ttatgcagat
    18661 gaatgacgtc gcaa
//
LOCUS       DBFIFM010000079        18656 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.079, whole genome shotgun sequence.
ACCESSION   DBFIFM010000079 DBFIFM010000000
VERSION     DBFIFM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.079"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(307..954)
                     /gene="ychE"
                     /locus_tag="N7C74_002384"
     CDS             complement(307..954)
                     /gene="ychE"
                     /locus_tag="N7C74_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HGU2432244.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(985..1038)
                     /gene="ychE"
                     /locus_tag="N7C74_002385"
     CDS             complement(985..1038)
                     /gene="ychE"
                     /locus_tag="N7C74_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HGU2432245.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1431..4106
                     /gene="adhE"
                     /locus_tag="N7C74_002386"
                     /gene_synonym="adhC"
     CDS             1431..4106
                     /gene="adhE"
                     /locus_tag="N7C74_002386"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HGU2432246.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            complement(4407..5024)
                     /gene="tdk"
                     /locus_tag="N7C74_002387"
     CDS             complement(4407..5024)
                     /gene="tdk"
                     /locus_tag="N7C74_002387"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HGU2432247.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            5628..6041
                     /gene="hns"
                     /locus_tag="N7C74_002388"
     CDS             5628..6041
                     /gene="hns"
                     /locus_tag="N7C74_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HGU2432248.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(6185..7093)
                     /gene="galU"
                     /locus_tag="N7C74_002389"
     CDS             complement(6185..7093)
                     /gene="galU"
                     /locus_tag="N7C74_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HGU2432249.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(7295..8308)
                     /gene="rssB"
                     /locus_tag="N7C74_002390"
     CDS             complement(7295..8308)
                     /gene="rssB"
                     /locus_tag="N7C74_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HGU2432250.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(8400..9305)
                     /gene="rssA"
                     /locus_tag="N7C74_002391"
     CDS             complement(8400..9305)
                     /gene="rssA"
                     /locus_tag="N7C74_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HGU2432251.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            9418..9876
                     /gene="ychJ"
                     /locus_tag="N7C74_002392"
     CDS             9418..9876
                     /gene="ychJ"
                     /locus_tag="N7C74_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HGU2432252.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            9926..10768
                     /gene="purU"
                     /locus_tag="N7C74_002393"
     CDS             9926..10768
                     /gene="purU"
                     /locus_tag="N7C74_002393"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HGU2432253.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVSRFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            10928..11012
                     /locus_tag="N7C74_002394"
     tRNA            10928..11012
                     /locus_tag="N7C74_002394"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10962..10964,aa:Tyr,seq:gta)
     gene            11057..11187
                     /locus_tag="N7C74_002395"
     ncRNA           11057..11187
                     /ncRNA_class="other"
                     /locus_tag="N7C74_002395"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            11222..11306
                     /locus_tag="N7C74_002396"
     tRNA            11222..11306
                     /locus_tag="N7C74_002396"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11256..11258,aa:Tyr,seq:gta)
     gene            11353..11484
                     /locus_tag="N7C74_002397"
     ncRNA           11353..11484
                     /ncRNA_class="other"
                     /locus_tag="N7C74_002397"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            11531..11662
                     /locus_tag="N7C74_002398"
     ncRNA           11531..11662
                     /ncRNA_class="other"
                     /locus_tag="N7C74_002398"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(11846..12523)
                     /gene="narI"
                     /locus_tag="N7C74_002399"
     CDS             complement(11846..12523)
                     /gene="narI"
                     /locus_tag="N7C74_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HGU2432254.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(12523..13233)
                     /gene="narJ"
                     /locus_tag="N7C74_002400"
     CDS             complement(12523..13233)
                     /gene="narJ"
                     /locus_tag="N7C74_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HGU2432255.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(13230..14768)
                     /gene="narH"
                     /locus_tag="N7C74_002401"
     CDS             complement(13230..14768)
                     /gene="narH"
                     /locus_tag="N7C74_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HGU2432256.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(14765..18508)
                     /gene="narG"
                     /locus_tag="N7C74_002402"
     CDS             complement(14765..18508)
                     /gene="narG"
                     /locus_tag="N7C74_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HGU2432257.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         4732 a         4873 c         4458 g         4593 t
ORIGIN      
        1 ctgattacag ttccagtagt gaaatgcaca ataatttaac aattgattat tatttagcac
       61 tttcctctac caaaggcagt ggaattacta atatcatttc aattattctt cagcaagccc
      121 aggattatga tgtggcgaaa ataacatgat gatgaggaga agcagttaag gaaatcctta
      181 taactaactg taatttgaga agaatatttt tacctattcc ttttgctttt gataactgcg
      241 cgggaacaaa gtaaaaataa tgacgaagta aacggagcat ggcagctccg tttcatttga
      301 aaggaattaa ttaagcaggc cggggaaaat ccccttaata ccagtaacga taaattcaat
      361 ccccaatgcc atcagcaata gccccataat acgcgtaatc acgttgatgc cggtctggcg
      421 taaaacccgt accagccacg gtgccatgcg gaacaatccc caacaacata aagcgaacaa
      481 tgcaatagcc acaaagaaac caaacagata gctaatgctg tgataacgcg taccccagac
      541 gatggtagaa ctgatcgccc ctggccccgc catcaacggc aacgccaatg gcaccacacc
      601 aatactttca cgtaccgcgg tttctgattt ttcttgcttg ttctgtttat cctcgccaag
      661 cttgccgctg atcatcgaca tcgctatcgt caccaccagg atgcccccgg cgatacggaa
      721 cgaatcaatt gatataccaa aaagttgcag aatcgtgtcg ccgagaaaaa gcgagatcca
      781 caagataatg gccacagaca ggttggctgt aaggttagtt ttgtttcgcg ctgccgctgt
      841 ctgataactg gtcatgctga taaagacggg aataatccct accgggttga ccagcgcaaa
      901 taacccgatg aaaaatttga agtaaacggg aaaatcaaaa agggtctgaa tcacggttag
      961 ctccgaagca aaagccggat aatgttagcc ataaataagg ttgaaaagac gcgctgacaa
     1021 tacgcctttt gacagcattt ttcacctcct aactacttaa aattgctatc attcgttatt
     1081 gttatctagt tgtgcaaaac atgctaatgt agccaccaaa tcatactaca atttattaac
     1141 tgttatctat aatggcgaaa agcgatgctg aaaggtgtca gctttgcaaa aatttgattt
     1201 ggatcacgta atcagtaccc agaagtgagt aatcttgctt acgccacttg gaagtgacgc
     1261 attagagata ataactctaa tgtttaaact cttttagtaa atcacagtga gtgtgtgcgc
     1321 gagtaagctt ttgattttca tcggttaagc aaatcatcac cgcactgact atactctcgt
     1381 attcgagcag atgatttact aaaaaagttt aacattatca ggagagcatt atggctgtta
     1441 ctaatgtcgc tgaacttaac gcactcgtag agcgtgtaaa aaaagcccag cgtgaatatg
     1501 ccagtttcac tcaagagcaa gtagacaaaa tcttccgcgc cgccgctctg gctgctgcag
     1561 atgctcgaat cccactcgcg aaaatggccg ttgccgaatc cggcatgggt atcgtcgaag
     1621 ataaagtgat caaaaaccac tttgcttctg aatatatcta caacgcctat aaagatgaaa
     1681 aaacctgtgg tgttctgtct gaagacgaca cttttggtac catcactatc gctgaaccaa
     1741 tcggtattat ttgcggtatc gttccgacca ctaacccgac ttcaacagct atcttcaaat
     1801 cgctgatcag tctgaagacc cgtaacgcca ttatcttctc cccgcacccg cgtgcaaaag
     1861 atgcgaccaa caaagcggct gatatcgttc tgcaggctgc tatcgctgcc ggtgctccga
     1921 aagatctgat cggctggatc gatcaacctt ctgttgaact gtctaacgca ctgatgcacc
     1981 acccagacat caacctgatc ctcgcgactg gtggtccggg catggttaaa gccgcataca
     2041 gctccggtaa accagctatc ggcgtaggcg cgggcaacac tccggttgtt atcgatgaaa
     2101 ctgctgatat caaacgtgca gttgcatctg tactgatgtc caaaaccttc gacaacggcg
     2161 taatctgtgc ttctgaacag tctgttgttg tcgttgactc tgtttatgac gcagtacgtg
     2221 aacgtttcgc aacccacggc ggctatctgt tgcagggtaa agagctgaaa gctgttcagg
     2281 acgttatcct gaaaaacggt gcgctgaacg cggctatcgt tggtcagcca gcctataaaa
     2341 ttgctgaact ggcaggcttc tctgtaccag aaaacaccaa gattctgatc ggtgaagtga
     2401 ccgttgttga tgaaagcgaa ccgttcgcac atgaaaaact gtccccgact ctggcaatgt
     2461 accgcgctaa agatttcgaa gacgcggtag aaaaagcaga gaaactggtt gctatgggtg
     2521 gtatcggtca tacctcttgc ctgtacactg accaggataa ccaaccggct cgcgtttctt
     2581 acttcggtca gaaaatgaaa acggctcgta tcctgattaa caccccggct tctcagggtg
     2641 gtatcggtga cctgtataac ttcaaactcg caccttccct gactctgggt tgtggttcct
     2701 ggggtggtaa ctccatctct gaaaacgttg gtccgaaaca cctgatcaac aagaaaaccg
     2761 ttgctaagcg agctgaaaac atgttgtggc acaaacttcc gaaatctatc tacttccgcc
     2821 gtggctccct gccaatcgcg ctggatgaag tgattactga tggccacaaa cgtgcgctca
     2881 tcgtgactga ccgcttcctg ttcaacaatg gttatgctga tcagatcact tccgtactga
     2941 aagcagcagg cgttgaaact gaagtcttct tcgaagtaga agcggacccg accctgagca
     3001 tcgttcgtaa aggtgcagaa ctggcaaact ccttcaaacc agacgtgatt atcgcgctgg
     3061 gtggtggttc cccgatggac gccgcgaaga tcatgtgggt tatgtacgaa catccggaaa
     3121 ctcacttcga agagctggcg ctgcgcttta tggatatccg taaacgtatc tacaagttcc
     3181 cgaaaatggg cgtgaaagcg aaaatgattg ctgtcaccac cacttctggt acaggttctg
     3241 aagtcactcc gtttgcggtt gtaactgacg acgctactgg tcagaaatac ccgctggcgg
     3301 actatgcgct gaccccggat atggcgattg tcgacgccaa cctggttatg gacatgccga
     3361 agtccctgtg tgctttcggt ggtctggacg cagtaactca cgccatggaa gcttatgttt
     3421 ctgtactggc atctgagttc tctgatggtc aggctctgca ggcactgaaa ctgctgaaag
     3481 aatatctgcc agcgtcctac cacgaagggt ctaaaaatcc ggtagcgcgt gaacgtgttc
     3541 acagtgcagc gactatcgcg ggtatcgcgt ttgcgaacgc cttcctgggt gtatgtcact
     3601 caatggcgca caaactgggt tcccagttcc atattccgca cggtctggca aacgccctgc
     3661 tgatttgtaa cgttattcgc tacaatgcga acgacaaccc gaccaagcag actgcattca
     3721 gccagtatga ccgtccgcag gctcgccgtc gttatgctga aattgccgac cacctgggtc
     3781 tgagcgcacc gggcgaccgt actgctgcta agatcgagaa actgctggca tggctggaaa
     3841 cgctgaaggc tgaactgggt attccgaaat ctatccgtga agctggcgtt caggaagcag
     3901 acttcctggc gaacgtggat aaactgtctg aagatgcatt cgatgaccag tgcactggcg
     3961 ctaacccgcg ttacccgctg atctccgagc tgaaacagat tctgctggat acctactacg
     4021 gtcgtgatta tgtagaaggt gaaactgcag cgaaaaaaga agccgctccg gctaaagctg
     4081 agaaaaaagc gaaaaaatcc gcttaatcag tagcgctgtc tggcaacata aacggcccct
     4141 tctggggccg tttttttgtt tacccaaagc aacttttcca taaaccgaca gcattagcct
     4201 tcatcatatt tgcgacgatg tataacgcct aaacacaggg atattgtact ttacaggtca
     4261 caagtcaacg tcggtgctta agagccctgt gaggcgtata gcggcgttaa aaactgccga
     4321 gaagggtgta tagcccggaa gaagtgcgta aaacgaactg acaggataaa agtgccctgc
     4381 tcaccctgtc agtaaagaaa ttcttattaa tcgtggcgat gcctttcctg aatagccgtt
     4441 aatgagccga cttgtaacgc ctctttatag tgtttacggc atacagaaac gtatcgttca
     4501 ttaccaccaa taaccacctg ctcaccttcg ttataaggtc tgcctgcttg atcaagacgc
     4561 agcaccatgc ttgctttacg gccacaaaaa cagatggttt ttaattcaac cagtttgtcg
     4621 gaccatgcca gtaagtattg gctgccaata aataattcac ctcgaaaatc ggtacgtaaa
     4681 ccataacaaa gtacgggtat atcgagttga tcgacaacct ccgataattc atatacttgt
     4741 tgtctggtta aaaactggca ttcatcaacc agtacgcaat gaattgcctg ctgctcatgt
     4801 tccgcacgaa tctcatcaaa taatgatgaa ttttggttaa ataattttgc aggcgatgac
     4861 aaacctatac gcgaactgac tttcccggca ccaaagcgat catcaatttc tgccgtatat
     4921 acgacagtgc gcatgccgcg ttcctggtaa ttgtatgaag attgcaacaa tgctgtagac
     4981 ttacccgcat tcattgcgga atagtagaaa tatagctgtg ccatcagcca caggccctca
     5041 atgatatgaa ataaagttgg ctggagttta tcataattcg tcagtttttc agctcaacag
     5101 tatgcacaga agtggtatat gcgttctccc ttacgaagcc ttgcataatc cttctgagct
     5161 atcattacaa ctgcctcgct tgttataagc gggtaaatga ctgctggtaa ctattcacaa
     5221 tctttaacct gttgcgcaag taatagccct ctgttgacct ccaggagata gtgcaatact
     5281 aagtccctgc tcttattgcg actgttctac ttttcatcat tcgcttaata gggaattctc
     5341 gtaaacacga ctaatacaga agactgaaag gtcgtcagcc tacgataatc tccccataaa
     5401 atgtgacatg aatcaggaag ttttaacctc acgtgctgcg aaatcatcgg tacaaatagg
     5461 gctatatgcc gcgtcttttc tggctaattt tatgaaaaga tatttattgg cggcacaaaa
     5521 taaagaacaa ttttgaattc cttacattcc tggctattgc acaactgaat ttaaggctct
     5581 attattacct caacaaacca ccccaatata agtttgagat tactacaatg agcgaagcac
     5641 ttaaaattct gaacaacatc cgtactcttc gtgcgcaggc aagagaatgt acacttgaaa
     5701 cgctggaaga aatgctggaa aaattagaag ttgttgttaa cgaacgtcgc gaagaagaaa
     5761 gcgcggctgc tgctgaagtt gaagagcgca ctcgtaaact gcagcaatat cgcgaaatgc
     5821 tgatcgctga cggtattgac ccgaacgagc tgctgaatag ccttgccgcc gttaaatctg
     5881 gcaccaaagc taaacgtgct cagcgtccgg caaaatatag ctacgttgac gaaaacggcg
     5941 aaactaaaac ctggactggc cagggccgta ctccagctgt aatcaaaaaa gcaatggatg
     6001 agcaaggtaa atccctcgac gatttcctga tcaagcaata atcttttgta gattgcactt
     6061 gcttaaaatc ccgccatcgg cgggattttt tattgtccgg tttaagacaa tttaataaga
     6121 ataagctata aaaaaacggc gtcgattgct caacgccgtt tcgtggataa caccgatacg
     6181 gatgttactt cttaatgccc atctcttctt caagccaggc tttaaattcc gtgccaaggg
     6241 tgttatgacg aataccgtat tcaacgaagg cctgcatgta acctaattta ttaccgcagt
     6301 catggctctt ccctttcata tgataggctt caaccgtttc tttttcgatt agcatatcaa
     6361 ttgcgtcggt gagctgaatt tcatcaccag ctcccggagg ggtttttgcc agcaacggcc
     6421 aaatatccgc actaagtacg taacgaccca caatagcgag attagacggc gcaacatccg
     6481 cttttggttt ttcaaccacg ccaaccatcg gtacgctttc acccggcgct aattcaacgc
     6541 ctttgcaatc cacaacgcca tatgcggtca catcagcaac cggttcaacc atgatctggc
     6601 tatgacccgt ttcatcgaag cggcggatca tctctgccag gttatcctgt gacaaatcgg
     6661 attcatattc atccagaata acgtcaggca aaataacagc cacaggttca tcacccacta
     6721 ccgggtgagc acacaatacc gcgtgtccca ggcctttcgc cagcccctga cgaacttgca
     6781 taatagttac gtgcggcgga caaatagact gcacttcatc aagcagttga cgttttacac
     6841 gtttttccag cattgcttcc agttcaaaac tggtatcaaa gtggttttca atagagtttt
     6901 tagatgagtg tgtaaccagc acaatttcag taatgccagc cgcaatacat tcattcacga
     6961 cgtattgaat taatggctta tcgacaagtg gcagcatctc tttcgggatg gctttcgtcg
     7021 ccggcaacat cctggttcct aatcccgcaa cggggataac ggcttttttg actttcgtat
     7081 taatggcagc catttaaatt ctcctggact gttcgtgttt tgaacgtgtt catatttctg
     7141 tatcgcatcc cagtatatca gcaccccgga ggggctaagt tctgaaaagg atgcgagtta
     7201 gcataattaa gacaattact tatacgaatg gcgacgatag taccaccagc cgacattagc
     7261 tggtaatgca aatttagccc gcgttatcgt ttgctcattc tgcagacaac atcaagcgca
     7321 gtcgaccacc ggttccccat atttggcatt gccaggcatc gcatcgctgg ctcaattgat
     7381 tcaaataagc gttacctaaa gtgcctaacg gaacaccatt actgatttgc acctggtgtt
     7441 cgccggtatt taacgtcgca ttcagacccg cagaaaccag aatgagattt ttcagttcgc
     7501 gatgataata gccaactaat agcggaaact gccccggcag attggcctga cggagtaaat
     7561 ggtttacctg cttcaataac gcgcccaact ctggcaaccg ttgattttgg tgtgcaagct
     7621 gttcctgtaa taatccgtta aacaatgcgc gtaataacaa ggcagcaagt acgccattat
     7681 gtccagctcg ggttacatca aggcaataaa atgccagatc gttttccgaa agtgcggcaa
     7741 tatcaagcac caggccgggt ttgtccgcgg caaccaattg acggtaatta acccggcaat
     7801 gggaaatcac ctgctgaacc ggcggttgta gttcctgtaa taatttcgcc gctgcggcag
     7861 ggttatcaac cattgcatcc cagtcgcgaa aaagcctttc ctcttcctca acgcgcgaat
     7921 taaacatgct gggatagaga caggcaaaaa ccatctcgcg caagcgattc agatctttaa
     7981 ctggtttcag caaaacatct tcaacgccca gacgtaacgc tttggcaata tctgccatat
     8041 tttcagtggc agatatcacc agaactgggg tctggtcgcc tctgttacgt atatgctcca
     8101 gcagtttaag cccgttcatt cgtggcatcg cgatatcaca tatcatcagg tctggagtga
     8161 aacctcccag caactcaagg gcatccaccc catcagccgc cagtaccgtt gtcgctccca
     8221 atgaggaaaa ccacgaatcc agaagcgagc gaaatacctg ctcatcttca acaatgagaa
     8281 tctgttttcc gaccaatggc tgcgtcatgt tctctcccct gactggcttt actcaatagt
     8341 ggcatgctat tgccacctgc gcctgtcaga attaacttaa gtgtagataa aaattctggt
     8401 caaatgttgg tgcgtaccaa cggcaaaagt tcgtccattt tcttttccac tgccagctgc
     8461 ccggccgcaa tggcagcgtg cgcgcgatgg aaatcaagcg tagatatttg cgggcaaaca
     8521 ggttgaatca gaatatcggg cggatcacct gccatgcggt tccttttaag gcggttctcc
     8581 agcacctgga tagaagtggt cataatctct gttgccgttg gtgctgtcac cgcccgacgt
     8641 gtcgttatac tgcctaacct ttctttcaga cgcgcatgcc acggcagaga atcaccattc
     8701 tcgctatttt cttcactgac attaaaggag agcaaatctt gttgcatcaa atgagcatcg
     8761 tgctgcaggt cgaccgctat cacaatatca gcacccaatg cacgcgtgag cgaaatagga
     8821 attgggttaa cgactgcccc atcaaccagc cagtagccgt tatgcgcaac gggtgccatt
     8881 agtcctggaa tactgcatga tgcgcgaata gcaagatgga gatcgccttc agtaaaccat
     8941 aattcacgcc cagtacttaa attggtggca acagccgcga agcgacggga acaattttcg
     9001 atctctgttt ccggcattat ttcgcggtat tgattgaaga cacgctcgcc gcgcagtaac
     9061 ccaccgcgct gccaggagag atccatcagt cgtaaaacat cccaataact gaaagaggtc
     9121 acccaatctt ccagcgcaga taatcgatcg catgcatagg ccgcgcccac cagcgaacca
     9181 attgaacatc ctgcaacgat atcaatttca atacccactt tttttagcgc attaataacg
     9241 ccaatatgcg accaacctct cgccgcgcca gatcccagcg ccagccctat ttttatcttt
     9301 ctcattattc ccgcaagatt cccctggaat gcaatcgtag ccacatcgct actgctcggt
     9361 taacatagtg ctactcaggc ggttaatacg ccgttattgt ttccagggag attatttgtg
     9421 tctcagcttt gtccctgtgg tagtgctgtc gagtatagcc tatgttgcca cccttatgtg
     9481 tctggtgaaa aggttgcacc tgatccagaa catctcatgc gttcgcgtta ctgcgctttt
     9541 gtgatgcaag acgcagatta tttaataaag acctggcatc catcttgtgg agcagcggcg
     9601 ttacgtgccg aattgattgc cggatttgcg catactgaat ggttgggatt gaccgtattt
     9661 gaacattgtt ggcaggacgc cgacaatatc ggatttgtca gttttgttgc tcgatttacc
     9721 gaaggtggca aaacaggtgc aataattgaa cgttcgcgat ttttgaaaga aaacggtcaa
     9781 tggtactata ttgacggtac acgtccgcag tttggccgca acgatccctg cccttgtggt
     9841 tcaggtaaaa aatttaaaaa gtgctgcggc caataatggt tgacggtacg gtttagcaaa
     9901 cactctcaac aaggtttttc cagcaatgca ttcactccaa cgtaaagttc tgcgtactat
     9961 ttgtccggac caaaaaggtc tgatcgcacg tattaccaat atttgctaca agcacgagtt
    10021 aaatatcgta cagaacaatg aatttgttga tcaccgtacc gggcgctttt ttatgcgcac
    10081 ggaactggaa gggattttta atgattccac cctgctggcg gatctcgata gcgcattgcc
    10141 agaaggctcc gtgcgtgagc tgaatcctgc cggtcgtcgc cggatagtga ttctggtcac
    10201 taaagaagcg cattgccttg gcgatttgtt gatgaaagcc aattatggcg gcctggatgt
    10261 cgaaatcgcg gcagtgattg gtaaccacga tactttacgt tctctggttg agcgttttga
    10321 tataccgttt gagctggtaa gccatgaagg gttaagccgc aacgagcacg atcaaaagat
    10381 ggcggatgcc attgatgctt atcaacctga ctacgtggtg ctggcgaagt atatgcgggt
    10441 attaacaccg gaatttgtgt cacgcttccc gaataagatc atcaatattc accattcctt
    10501 cctgccagcg tttatcggcg cacgtcctta tcaccaggcc tatgaacgtg gcgtgaagat
    10561 tattggcgca accgctcact atgtgaatga caatctggac gaaggcccaa tcatcatgca
    10621 ggacgttatt catgtcgatc atacctacac agctgaagat atgatgcgcg caggtcgtga
    10681 cgtcgagaaa aacgtcttaa gtcgcgcgct ctacaaagta ctggcgcagc gcgtctttgt
    10741 ttacggtaat cggacgatta ttctttaatc gccagcaaaa ataactggtt acctttaatc
    10801 cgttacggat gaaaattacg caaccagttc atttttctca acgtaacact ttacagcggc
    10861 gcgtcatttg atatgatgcg ccccgcttcc cgataaggga gcaggccagt aaaaagcatt
    10921 accccgtggt ggggttcccg agcggccaaa gggagcagac tgtaaatctg ccgtcatcga
    10981 cttcgaaggt tcgaatcctt cccccaccac catgattcac cacagcgatg tggtaacttc
    11041 ggtgagattg tgaaagtttg cactcatcgg ggaagggtga gaaccttcga ctaaggttcg
    11101 actcgagcga aagcgagaga acgttttcac aggcaacggc ccgaagggtg aggagcgaag
    11161 cgacgaataa tcctttcccc accgccatct caagcattac ctcaaaattt gaattacccc
    11221 tggtggggtt cccgagcggc caaagggagc agactgtaaa tctgccgtca tcgacttcga
    11281 aggttcgaat ccttccccca ccaccatcac tttcaaaagt ccctgaactc tcaagcgaat
    11341 ccgtaaataa atattctgcc caagcggaga aggatgagaa gcttcgacta aggttcgact
    11401 cgagcgccag cgagagagcg ttgccgcagg caacgacctg aagggcgaag cgcgcagcgc
    11461 tgagtaatcc ttcccccacc accatcactt tcaaaagtcc ctgaactctc aaacgaatcc
    11521 gtaattaaat attctgccca agcggggaag gatgagaagc ttcgaccaag gttcgacttg
    11581 agcgccagcg agagagcgtt gccgcaggca acgacccgaa gggcgaagcg cgcagcgctg
    11641 agtaatcctt cccccaccac catcgcttcc tgaaataatc aaaacctgtt actccgagct
    11701 aattttgccc aatggcacca cgtttaccgg acctacaata tcctcccttg taggtcggat
    11761 aagcgaagcg catccgacaa ctcaccccgc accgtgccca taaaaaaacc ccgccgaagc
    11821 agggtctatg tgaactaaaa ttcgcttagt gacgagcgcg caccagctgg tacttacgtg
    11881 tcagatactc caccggtacg ctccagatgt gtaccagacg cgagaacggg aacagcagga
    11941 ataacgtcat ccccagcacc aggtgtagac ggaagataaa cgccacacca tcgaggtgtt
    12001 gagaagcgcc accgtggaag gtcactaccg actgcgccca gccaaccagt ttcatcatct
    12061 cgctaccgtc catatgttgg gcggagaacg gaatggtcaa caggcccagc gcgcactgga
    12121 taacgagcag cgacaggatc agtatatccg ctccggtagt cgttgcacgc acgcgtgggc
    12181 tgaacagacg acgtttaagc aaaagcacgc cgccaatcag acacagcacg ccgctggcac
    12241 caccagcaaa catcgccatt ttctgtttca cttcaatcgg cagccaggct tcatacatcc
    12301 agtgcggcgt caacataccg aagaagtgac cgacaaaaat ccccagaata ccgatatgga
    12361 acaggttcga cgccaggttc atccctttgc gatccagcat ctggctggac gccgcgcgcc
    12421 aggtgtactg cccgtagtca taacgcagcc agctaccaat caggaagacc gccccggcta
    12481 tgtacgggta gatatcaaag aagaacatat tcaggaattg cattagtgct gtcctccggt
    12541 ggtgatattc agatattgcg gcgcgacggc accggcaaag cgacgctgat gagcagtgat
    12601 tgctgaatca ccgcagcctt tgtcagcaaa gaatttaacc tgctcttctt cccaaacagc
    12661 atccagcgcc tgcggcgtat catcgcgcgc ttcgtcggca attttttccg ccactttgtc
    12721 gctgtcgata gcggtattgg ccaatttcag cagcagatca aacagcacgg cataacggct
    12781 ttcacgctgt tgcagacgcg cactcagcaa tgccagaatc ggcgcgatat ctttcaaacc
    12841 ttccacggct tcgctttgcg gcagctgcgc caggtactcc agatacagcg gcagatgatc
    12901 cggcaattcg cggctgttta actgcaagcc gtgctgctcg tactgcgcca gcaggtccac
    12961 catcgcctgg ccgcggtcgc gggattcgcc gtgcacatgt tcaaacagca acagtgacgt
    13021 ggcgcggcca cggtcgaaca gttcgctgta ctgcgcctgg gcatcgagcg gatccatcgt
    13081 cgttaaatcg cgcaggaaaa tgcccagcgc atgggcatcc tcttttggca gatttttcga
    13141 cgcggcaatc gcctcaaaca tctcttgttg atgctgccat aaggcagcat ccggatattc
    13201 aaggagacgc gatacaatca cgagttcgat catggatgcg gctccgtttt gctggtcaca
    13261 tcgatggcat cgatacgacg gctgttgaac agattgaatt tggtatctga accgtggcag
    13321 ccatcaccaa aggtaaagcc gcagccattt ttctccggga aggcttcccg tgccagttca
    13381 cgatgactac tcggcaccac aaagcgatct tcgtagttag caatcgccag ataacggtac
    13441 atctcctgtg cctgggcttc ggtcagaccg acctcttcca gcgcacgggt atcaacttta
    13501 ccgtcaacgg tttcagcacg tttgtaatga cgcatcgcca gcatacgttt cagcgcgcgc
    13561 agtaccggtt tggtatcacc ggcggtcagc agattcgcca gatactgtac cggaatacgc
    13621 aggctttcga cgtctggcag aatgccgttg ctacccagct cacccgcgtc tgctgcagac
    13681 tgaatcggag agagaggcgg cacgtaccag accatcggca gtgtgcgata ttctggatgc
    13741 agcggcagcg ccagtttcca ttccattgcc attttataaa ccggcgactg ctgtgcagct
    13801 tcaataacgc tcagcggaat accgtcttta atcgcctgct cgatgacttt cggatcgttc
    13861 ggatcgagga acacctccag ctgacgctgg tagagatctt tctcgttctc ggtgctggct
    13921 gcacgttcaa tcgcgtcggc atcgtacagc agcacgccaa gataacggat acgaccgaca
    13981 caggtttctg agcacacggt cggctgaccc gcttcaatac gcggatagca gaagatgcac
    14041 ttctcagact taccgctctt ccagttgaag tagatttttt tgtacgggca tccggtgatg
    14101 cacatacgcc agccacggca tttatcctgg tcgatcagga cgatgccatc ttcttcacgc
    14161 ttgtaaatcg caccgctcgg gcaggtcgcc acacatgccg ggttcaggca gtgttcgcac
    14221 aggcgcggca aatacatcat gaaggtgttt tcgaactggc tatacatcgc cttctggatg
    14281 ttgtcgaagt tcttgtcttt cgccagtttg tcaaactcac cgcccaggtc atcttcccag
    14341 ttcggccctt tttcgatttt cgccatccgt tccccggtaa tcagcgaacg cggacgggca
    14401 atcggctgcg atttgctgcc ttccggcgcg gtatgcaggt tctgatagtc aaaatcgaac
    14461 ggctcgtaat aatcgtcgat ccccggcaga tgcgggttag cgaagatttt acccagcagc
    14521 atggcacggt tacccatgcg cggttgcagt ttgccgttga ttttacggat ccagccgcct
    14581 ttgtattttt cctggttttc ccagtcagtc gggaagccct ggcccggctt ggtttccacg
    14641 ttgttgaacc acgcgtattc cacgccttca cggctggtcc agacgttttt acaggtaact
    14701 gaacaggtgt ggcagccgat gcacttatcg agattcagca ccatgccgac ttgtgaacga
    14761 attttcattt tacgctctcc tgtacctgat cattgccttc gccatctaac cagtcaatgt
    14821 tcttcatctt acgcactaca acaaactcat cgcggttaga acctacggtg ccgtagtagt
    14881 taaagccgta tgccagatgg gcatagccgc cgatcatatg cgtcggtttc ggcgtgatac
    14941 gggtgaccga gttatggata ccaccacgct gttgggtaat ttccgaacca ggcaggttaa
    15001 cgatacgttc ctgcgcgtgg tacatcatgg tcatccctgc cggaacacgc tggctgacaa
    15061 ccgcacgggc agtcagagca ccgttgctgt tgaatacttc aatccagtcg ttatcggcga
    15121 tacccagatc tttggcatcg gcttcactca accagaccac cggaccaccg cgacctaaag
    15181 tcagcatcag caggttgtcg ctgtaggtgg agtggatacc ccacttctgg tgcggcgtca
    15241 ggaagttgag cgctttttcc gggttgccgt tggatttctg gcctatcact tctttcaccg
    15301 aacgggtgtc gatcggcgga cgataaacca gcaggctttc accgaaatca cgcatccact
    15361 ggtgatcctg atacagttgc tgacgaccag agagcgtacg ccatgggatc agctcgtgaa
    15421 cgttggtgta accggcgttg taagaaacgt gttcatcttc cagaccagac caggtcgggc
    15481 tggagataat tttgcgcggc tgtgcctgaa tatcgcggaa gcggatcttc tcgtcttctt
    15541 tattcagcgc cagatgcgta tggtcacgac cggtaaattc gctcagggca gcccaggctt
    15601 tcacggctac ctgaccgttg gtttccggtg ccagtgtcag gatcatctct gccgcatcaa
    15661 ttgcggtgtt cagcatcggc tggcctttcg ccggaccttc cgctttggtg tagttgagct
    15721 tacgcagcag atccatctcg ctctgggtgt tccaggcaat ccctttaccg ccgttaccga
    15781 ttttctccat cagcgggccg atagaggtaa agcgttcgta agttgccgga taatcgcgct
    15841 ctacgaccat aatgtgcggt gcagttttgc ctgggatcag gtcgcattcg ccttttttcc
    15901 agtctttcac atccagcggc tgcgccagtt cagcggcaga gtcgtgctgg ataggcagcg
    15961 tgacgatgtc ggtttcttta cccagatggc cgacgcacac ttcggagaat ttcttcgcga
    16021 tggctttgta gatttcccag tcgcttttcg cttcccaggc cggatcgacc gccgcagaca
    16081 gcgggtgaat aaacggatgc atatccgaag tattcatgtc gtctttttcg taccaggtcg
    16141 ccgtcggcaa aatgatgtcg gaatagagac aggtgctcga cagacggaag tccagcgtaa
    16201 ccaccagatc cagcttgcct tccagaccat tgtcctgcca gtccacttct tccggcttca
    16261 cgccgccctg ctgccccaga tctttaccct ggataccgtg ctccgtcccc agcaggtact
    16321 tgagcataaa ctcatgacct ttaccggaag aaccgagcag gttagaacgc cagataaaca
    16381 ggttacgcgg gtggtttttg ccgttttccg gctgttccgc agcgaaacgg atagaaccct
    16441 ctttcaggga tttcaccgta tagtccaccg gattcatccc ggctttttct gcttcgccag
    16501 cgatagtcag cgggttagtg cctaactgcg gcgcagacgg cagccagccc atgcgttctg
    16561 cacggacgtt aaagtcgatc agatgtccgg tatagcggga tttatccgcc atcggcgaca
    16621 gcagttcttc cgcagtcacg gtttcataac gccactggct ggagtggtta tagaaataag
    16681 aggtgctgtt catgtgacgc gccggacgct gccagtcgag ggcaaacgcc agcggctgcc
    16741 agccggtttg cggacgcagt ttttcctgac ctacatagtg cgcccagccg cccccgctct
    16801 gaccgacaca gccgcagaaa atcagcatgt tgatcagacc acgatagttc atatcgaggt
    16861 gataccagtg gttcagcccc gcaccgacga taatcatcga acgaccgtgc gttttatcag
    16921 cgttatcggc aaattcacgg gcgatgcgaa taatctggct gcgagaaacg ccggtaatct
    16981 gctcggccca ggccggggta taagctttca catcgtcata gctggttgca cagttaacgt
    17041 cgttcaggcc acgttccaga ccgtagtttg ccagcgtcag atcataaacg gtggtcacca
    17101 gggcggtgct gccatcagcc agttgcaggc gtttcaccgg cagtttgtgc agcagcacgt
    17161 tttccagttc cactttgttg aagtgttccg tgccgtcgcc accaaagtac gggaagccta
    17221 cctcagcgat ctcatcctgg ctacccagca ggctcagttg cagctcggtt tcttcgccag
    17281 ttttgccgtc gcgctgctca agattccatt tgcccttctc gccccagcgg aagccaatag
    17341 aaccgttcgg cgcaaccatt tcgccattgg tattaaaggc gacagttttc cattccggat
    17401 tgttttcctg gcccagcgca tcaaccagat cagcagcgcg cagcatacga cctgcagcgt
    17461 agtaaccgtc gcgttcttcc agcatcacca gcatcggcat gtcggtgtag cgacgcacat
    17521 agtcggtgaa atactggctt gggttgtcga gatggaattc acgcagcatt acgtggccca
    17581 tcgccagcgc cattgccgca tcggtgccct gtttcggtgc cagccacaga tcgcacagtt
    17641 tggcgatttc agcgtagtct ggtgtgacgg caacagtttt ggtgcctttg taacgcactt
    17701 cagtaaagaa gtgagcatct ggagtacgcg tctgcggcac gtttgaaccc caggcgatga
    17761 tgtagctgga gttgtaccag tcagcagatt ccggtacgtc agtttgctcg ccccaggttt
    17821 gcggagacgc aggaggcagg tcgcagtacc agtcgtagaa gcttaagcaa gtaccgccaa
    17881 tcagcgagag atagcgtgca cccgatgcgt aagaaaccat cgacattgcc ggaattggcg
    17941 agaaaccagc aacacggtcc gggccgtagt ttttgatggt gtaaacgtta gatgcggcga
    18001 tcagttcgtt cacctcctgc caggaagaac gaacaaaacc accgcgtcca cgcgcctgct
    18061 taaagctttt cgctttatcg gcgtcttcaa tgatagaagc ccatgcctca accggatcgc
    18121 tatgcagcgc cttcgcttca cgccacattt tcatcaggcg tttgcgcatc atcgggtatt
    18181 tcaggcggtt ggcactgtaa agataccagg agtagctggc accgcgcggg cagccgcgag
    18241 gttcatggtt tggcagatcc ggacgggtac gcggatagtc agtctgctgg gtttcccagg
    18301 tgaccagacc gtttttgacg tagattttcc agctgcagga gccggtgcag tttaccccgt
    18361 gggtagagcg gacgattttg tcatgctgcc aacgctggcg atatccatcc tcccagtcac
    18421 ggttggtatt gagaagctgg ccatgcccat cggcaaaggt ttcacccttc tgtttgaagt
    18481 agcgaaaccg gtccaggaat ttactcatcg gttttctcct gtgggagcct gtcggccctc
    18541 tgataaatcg acattgctaa tgtttaaggg caaggtaacg ctctgaaaca gcgtgggaat
    18601 tgataacgat caagagtgat ggggatgaaa aataaagtaa attcctaaga tacccc
//
LOCUS       DBFIFM010000080        18540 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.080, whole genome shotgun sequence.
ACCESSION   DBFIFM010000080 DBFIFM010000000
VERSION     DBFIFM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.080"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            138..443
                     /locus_tag="N7C74_002403"
     CDS             138..443
                     /locus_tag="N7C74_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432258.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            550..1194
                     /gene="gfcB"
                     /locus_tag="N7C74_002404"
     CDS             550..1194
                     /gene="gfcB"
                     /locus_tag="N7C74_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HGU2432259.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLHIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1191..1937
                     /locus_tag="N7C74_002405"
     CDS             1191..1937
                     /locus_tag="N7C74_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HGU2432260.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VAQLVTQPQLRDRLWWPGALLTDSAVKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGNYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVIVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            1937..4033
                     /locus_tag="N7C74_002406"
     CDS             1937..4033
                     /locus_tag="N7C74_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HGU2432261.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4079..5218
                     /locus_tag="N7C74_002407"
     CDS             4079..5218
                     /locus_tag="N7C74_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HGU2432262.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5206..5652
                     /gene="etp"
                     /locus_tag="N7C74_002408"
                     /gene_synonym="yccY"
     CDS             5206..5652
                     /gene="etp"
                     /locus_tag="N7C74_002408"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HGU2432263.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            5672..7852
                     /gene="etk"
                     /locus_tag="N7C74_002409"
     CDS             5672..7852
                     /gene="etk"
                     /locus_tag="N7C74_002409"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HGU2432264.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(7972..9276)
                     /gene="appA"
                     /locus_tag="N7C74_002410"
     CDS             complement(7972..9276)
                     /gene="appA"
                     /locus_tag="N7C74_002410"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HGU2432265.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPEPELKLESVVIVSRHGVR
                     APTKATQLMQDVTPDAWPNWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLTKKGCP
                     QPGQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLD
                     NANVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQAL
                     PSELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNA
                     QFYLLQRTPEVARSRATPLLDLIMIALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANL
                     GGALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSL
                     NTPPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9356..9448)
                     /gene="cbdX"
                     /locus_tag="N7C74_002411"
     CDS             complement(9356..9448)
                     /gene="cbdX"
                     /locus_tag="N7C74_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HGU2432266.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(9461..10597)
                     /gene="appB"
                     /locus_tag="N7C74_002412"
     CDS             complement(9461..10597)
                     /gene="appB"
                     /locus_tag="N7C74_002412"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HGU2432267.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARDDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLSTKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(10609..12153)
                     /gene="appC"
                     /locus_tag="N7C74_002413"
     CDS             complement(10609..12153)
                     /gene="appC"
                     /locus_tag="N7C74_002413"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HGU2432268.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPVLLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12287..13144)
                     /locus_tag="N7C74_002414"
     CDS             complement(12287..13144)
                     /locus_tag="N7C74_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HGU2432269.1"
                     /translation="MSETFFHLLGPRTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13141..13539)
                     /gene="hyaE"
                     /locus_tag="N7C74_002415"
     CDS             complement(13141..13539)
                     /gene="hyaE"
                     /locus_tag="N7C74_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HGU2432270.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            complement(13536..14123)
                     /gene="hyaD"
                     /locus_tag="N7C74_002416"
     CDS             complement(13536..14123)
                     /gene="hyaD"
                     /locus_tag="N7C74_002416"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HGU2432271.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPESVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14120..14827)
                     /gene="hyaC"
                     /locus_tag="N7C74_002417"
     CDS             complement(14120..14827)
                     /gene="hyaC"
                     /locus_tag="N7C74_002417"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HGU2432272.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            complement(14846..16639)
                     /gene="hyaB"
                     /locus_tag="N7C74_002418"
     CDS             complement(14846..16639)
                     /gene="hyaB"
                     /locus_tag="N7C74_002418"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HGU2432273.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPTTWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(16636..17754)
                     /gene="hyaA"
                     /locus_tag="N7C74_002419"
     CDS             complement(16636..17754)
                     /gene="hyaA"
                     /locus_tag="N7C74_002419"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HGU2432274.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATSIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            18180..18267
                     /locus_tag="N7C74_002420"
     tRNA            18180..18267
                     /locus_tag="N7C74_002420"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18214..18216,aa:Ser,seq:tga)
BASE COUNT         4532 a         4939 c         4815 g         4254 t
ORIGIN      
        1 ctgcgcgcct ttctcacccg ctgtatatag tgcaagtggt atgaggaaag ggagatttca
       61 caaggatgtt atactcaaag tagacaatcc tgatctcaag cgtacgtatt gtccatgcga
      121 tgaataaagg aaaagttatg aaacacaagc tttctgcaat ccttatggcc ttcatgttga
      181 cgacgccagc tgcgtttgct gcccctgaag cagcaaacgg gaccgaggca acgacgggta
      241 ctactggcac cacaacgacc actaccggtg caaccacgac ggctgctacc actggtggcg
      301 tggctgctgg cgctgtcggc acggcgacag ttgtcggtgt ggcaacggct gttggtgtgg
      361 cgacgttggc agtagttgca gcaaatgaca gtggcgatgg cggttctcat aacacctcaa
      421 ccaccacgtc cactacgcgc taattctgct taaacgcgtc gaaattaaag ttaaataata
      481 cccggttgcg atattcgtac cgggctttag agatttgcat ttttttaatt aacctttcaa
      541 ggattaaagg tgcgccctct tattttatcg attttcgcac tatttcttgc gggatgtacg
      601 cacagccagc aaagtatggt cgatacattt cgcgccagcc ttttcgataa tcaggatatc
      661 accgtagcgg atcagcagat ccaggcgttg ccttattcca cgatgtattt acgccttaat
      721 gaagggcaac gaatctttgt ggtactggga tatatagaac aagaacaaag caaatggtta
      781 tcccaggata acgccatgct ggttacccac aatggacgtc ttttaaagac cgtcaaactt
      841 aataataatc tgctggaagt gacaaattcc gggcaggacc ctctgcgtaa cgcgctggca
      901 ataaaagacg gcagccgctg gacgcgcgat attctctgga gtgaagacaa ccattttcgc
      961 tctgcgaccc tgagttccac tttttccttt gctggattag agacgctgca tattgcgggt
     1021 cgcgatgtgc tgtgtaatgt ctggcaggaa gaggtgactt ccacgctacc agaaaaacag
     1081 tggcaaaaca cattctgggt cgattcggct actggccagg ttcgtcaaag tcggcaaatg
     1141 ttaggcgcag gggttattcc cgtagaaatg acgtttctta aacccgcacc atgaataaat
     1201 tacagtcgta tttcattgcc agcgtacttt acgtaatgac accccatgcc tttgcgcaag
     1261 gaacggtgac tatttatctg cctggcgaac aacagacact ttccgttggc cccgtggaga
     1321 atgttgccca gttagtgaca caaccgcaac tgcgcgatcg cctctggtgg cctggtgccc
     1381 tgctgaccga ctccgcggtg aaagctaaag cgcttaaaga ctaccaacat gtcatggcac
     1441 agcttgcttc ctgggaagca gaagctgacg acgatgtggc ggcaacgatt aaatccgtgc
     1501 gccagcagtt actcaatctc aatattactg gtcgcttacc ggtcaaactg gaccctgatt
     1561 ttgtacgagt ggatgagaac agcaatcccc cgctggtcgg gaactatacg ctctataccg
     1621 tacagcgccc ggtgactatt actcttctgg gtgcggtttc tggggctggg caattgccct
     1681 ggcaggcagg ccgtagcgtt accgattatc tgcaagatca cccccgtctt gcaggcgcgg
     1741 acaagaataa cgttatcgtg attacgcctg agggtgagac tgtcgtcgcc cctgttgccc
     1801 tgtggaataa aagacatgtg gaaccaccgc ctggcagcca gctttggtta ggcttttctg
     1861 cgcatgtact gcccgaaaaa tatgctgatt taaatgacca gattgtgtct gttttaacgc
     1921 aacgtgttcc tgactgatga agaagaattc ttatctttta agctgcctgg ccattgccgt
     1981 ctccagtgcc tgtcatgctg aagtattaac ctacccggat ccgctgggtt cgtcgcaatc
     2041 ggactttggc ggcacaggat tgttgcagat gccaaatgcg cgcatcgcac cggaaggtga
     2101 attcagcgtc aactaccggg ataacgatca ataccgtttc tactccacct ccgtggcgct
     2161 gtttccatgg ctggaaggca ccattcgtta tacggatgtg cgcacacgta aatatagcca
     2221 gtgggaagat ttcagcggcg atcagtcata caaagacaaa tcattcgatt ttaaacttcg
     2281 cctgtgggaa gaaggttact ggctaccgca agtggcgttt ggtaaacgtg atattgctgg
     2341 tacgggtctg tttgacggtg agtatctggt ggccagcaag caagcggggc catttgattt
     2401 caccctcggg atggcatggg gctacgccgg taatgcgggc aatattacca acccgttttg
     2461 ccgggtgagc gataaatatt gtcatcgcgc agagtctcac gatgcgggcg atatcagctt
     2521 tagcgatatc tttcgtggcc cggcttccat ctttggcggc attgagtatc aaacgccgtg
     2581 gaatcccctg cgtctgaaac tcgaatacga cggcaacaat taccagaatg atttcgctgg
     2641 caaactgcct caggcaagcc atttcaacgt cggtgcagtt tatcgcgctg ccagctgggc
     2701 agacctcaac ctgagttatg aacgcggtaa cacgttgatg tttggtttca ctttacggac
     2761 caatttcaac gatctgcgcc ctgccctgcg cgatacgcca aaaccggcat atcaacctgc
     2821 gcctgaatct gaaggattgc agtacaccac ggtagcaaac caacttaccg ccctgaagta
     2881 taacgcgggc tttgacgcgc cagaaattca gctacgcgat aagacgctgt atatgtctgg
     2941 tcagcaatac aaataccgtg actctcgtga agcggtcgat cgtgccaacc ggattctggt
     3001 gaataacctg ccgcaaggcg ttgagaagat tagcgtgacg caaaagcgcg agcatatggc
     3061 gatggtgact accgaaaccg acgtagccag cctgcgcaaa cagctggcag gtacagcgcc
     3121 tggtcaatca gagccactgc aacaacaacg tgttgaagct gaagatcttt ctgcctttgg
     3181 tcggggctac cgtattcgtg aagatcgctt tagctactct ttcaacccaa cactttcaca
     3241 gtcgctgggc ggcccggaag atttctatat gttccagctg gggctgatgt ccagcgcccg
     3301 ctactggttt accgaccacc tgctgcttga tggcggtatt ttcaccaata tttacaacaa
     3361 ctacgacaag tttaagtctt cgctgttgcc cgcggactct accctgcccc gcgtgcgcac
     3421 gcatatccgt gattacgttc gcaatgacgt ttatctcaac aacttgcaag cgaattactt
     3481 tgccgactta ggcaatggtt tctatggcca ggtgtatggc ggttatctgg aaacgatgta
     3541 tgccggtgtc ggttccgagc tgctttatcg cccgctagat gccagctggg cgctgggtgt
     3601 ggacgttaac tacgtgaagc agcgtgactg ggacaacatg atgcgcttca ccgattattc
     3661 cacgccaact ggtttcgtga cggcttactg gaacccgccg acgctcaatg gcgtactgat
     3721 gaaacttagc gttgggcaat atctggcaaa agataaaggg gcaacgatcg acgtcgccaa
     3781 acgctttgac agcggcgtgg cggtaggggt atgggcggca atcagtaacg tatctaaaga
     3841 tgactacggc gaaggcggct ttagtaaagg tttttatatc tcgattccgt tcgacttgat
     3901 gaccattgga cctaaccgca accgcgcggt ggtttcgtgg acaccattga cgcgtgatgg
     3961 tggacaaatg ctgtcacgca aataccagct ctatccaatg acggcagagc gagaagtacc
     4021 ggttggacaa taacctgacg taattcataa cattacattg actgataaat ataaaccaat
     4081 gaaaaagaac atttttaagt tttcggtact gacattggct gttctgtctt tgacagcatg
     4141 tacccttgtt cctggtcaga atttatcgac cagtaacaag gatgtcattg aactacctga
     4201 caatcagtat gacctggaca aaatggtcaa tatctatccc gtcacgccag gattgattga
     4261 tcagttgcgc gccaaaccga ttatgtcgca ggctaacccg gagctggagc agcaaatcgc
     4321 gaactatgaa tatcgtatcg gcattggtga tgtgttgatg gttacggtct gggatcaccc
     4381 ggaactaacc acccccgcag ggcaataccg tagcgccagt gataccggca actgggttaa
     4441 cgccgatggt gcgattttct acccctatat tggtcgcctg aaagtggccg ggaaaacact
     4501 tacccaggta cgcaacgaga ttacagcgcg tctggattca gtcattgaaa gcccgcaagt
     4561 cgatgtcagc gtcgcggcgt tccgctcgca aaaagcgtac gtcaccggtg aagtcagcaa
     4621 atcgggtcag cagccgatta ccaatattcc tctgaccatc atggatgcga ttaacgccgc
     4681 gggcggtctg accgcagatg cagactggcg caatgtggtg ctcacccaga atggcgtaaa
     4741 gaccaaagtg aacctctatg cgctgatgca gcgtggcgac ttgcgccaga acaaactgct
     4801 gcacccggga gatattttat ttattccgcg caacgatgat ctgaaagtct tcgtgatggg
     4861 tgaagtgggt aaacagagca cgctgaaaat ggaccgcagc ggtatgaccc tggcggaagc
     4921 gctgggtaac gctgagggta tgaatcagga tgtcgctgat gcgacgggga tttttgttat
     4981 tcgtgcaacg caaaacaagc agaatggcaa aatcgccaat atttatcagc tgaatgcgaa
     5041 ggacgcatcg gcaatgatac tcggaacgga attccagctt gaaccttacg acattgttta
     5101 tgtcactacc gcgcctctgg ctcgttggaa ccgtgtgatt tccctgctgg taccgacgat
     5161 ttccggggtg catgacctga cagagacttc acggtggatt caaacatggc ccaactaaaa
     5221 tttaactcaa tcctggtggt ttgtaccggc aacatttgcc gttcgcccat tggcgagcgc
     5281 ttgctgcgta aacgtctgcc gggcgtaaaa gtcaaatcgg caggtgttca tggtctggta
     5341 aaacaccctg cagatgcgac agcggcagat gttgccgcta atcatggcgt atctcttgaa
     5401 ggacatgccg gacgtaagct cactgctgag atggcacgaa attacgatct gattctggca
     5461 atggagtcgg aacatattgc tcaggttacc gcaatcgctc ccgaggtccg cgggaagaca
     5521 atgctgtttg ggcaatggct ggaacagaaa gagatcccgg atccctatcg taaaagtcag
     5581 gatgcatttg aacatgtcta cggaatgttg gagcgcgcca gtcaggaatg ggcgaagcgc
     5641 ctcagccggt aatttgagtt tataaaatac gatgacaact aaaaatatga atacgccacc
     5701 aggcagcact caggaaaatg agatcgatct gcttcgtctg gtcggcgagt tatgggatca
     5761 ccgtaagttt attatcagcg tgaccgcgtt attcacgctg atcgctgtcg cttactcgct
     5821 gttaagcaca ccaatttatc aggcagatac tctggtccag gttgagcaaa aacagggcaa
     5881 cgccattctc agcggcctga gtgatatgat ccctaactca tcgcccgagt ctgcaccgga
     5941 gatccaactg ctgcaatcgc gcatgattct cggtaaaacc attgctgaac tgaatctgcg
     6001 cgacatggtt gagcagaagt attttccgat tgtgggtcgc ggctgggcga gattaaccaa
     6061 agagaaacca ggtgagctgg cgatcagctg gatgcatatt ccacaactga atggtcagga
     6121 tcagcaactg acactcacgg ttggggaaaa cggccactat acgctggaag gtgaagagtt
     6181 caccgtcaat ggtatggtcg gccagcgtct ggaaaaagat ggcgttgcgc tgactatcgc
     6241 ggatattaag gccaaaccag gaacacagtt tgtcctgagc caacgtaccg aactggaagc
     6301 gattaacgcg ttgcaggaaa cctttaccgt tagcgaacgc agtaaagaaa gcgggatgct
     6361 ggaacttacc atgactggtg atgatcccca gttgattact cgtattctga acagcatcgc
     6421 taacaactat ttgcaacaga atatcgctcg ccaggcggcg caggattcac aaagccttga
     6481 attcttacag cgccagttgc cagaagtgcg cagcgagctg gaccaggcgg aagaaaaact
     6541 caacgtttat cgccagcagc gcgattcggt tgaccttaac ctggaagcca aagccgttct
     6601 tgagcagatt gtgaacgttg ataatcaact caatgagctg accttccgcg aggcagagat
     6661 ctcccagctg tataagaaag atcacccaac ttatcgtgcg ctgctggaaa aacgccagac
     6721 gctggagcaa gaacgcaaac gcctgaataa gcgggtatcg gctatgcctt ccacccaaca
     6781 ggaagtgttg cgtttaagtc gtgacgtaga agcgggccgt gcggtatatc tgcaattact
     6841 taaccgccag caggagttga gtatttcgaa atccagtgcc attggtaacg tgcggattat
     6901 cgacccggca gtcactcagc cgcagccagt gaaaccgaaa aaagcgttga atgtggtgct
     6961 tggttttatt cttggcctgt ttatttcggt gggtgccgtg ctggcgcgtg cgatgttgcg
     7021 tcgtggtgta gaagccccgg aacaactgga agagcacggc atcagcgttt atgccaccat
     7081 cccgatgtcc gagtggctgg ataaacgtac ccgtctgcgt aagaaaaatt tattttctaa
     7141 tcagcagcgc catcgtacta aaaatatccc cttcctggcg gtggataacc cggcggattc
     7201 tgctgtggaa gccgtacgtg cgctacgaac cagtctgcat ttcgctatga tggagactga
     7261 gaataacatt ctgatgatca ccggtgcgac gccagacagt ggtaaaacgt ttgtcagttc
     7321 aactctggca gcggtgatcg cccagtccga tcaaaaagtg ttatttattg atgccgactt
     7381 acgccgtggt tattcgcata acctgtttac cgtgagtaat gaacatggct tgtcggaata
     7441 tctggcaggt aaagatgagc tcaacaaagt gatccagcat tttggcaaag gaggctttga
     7501 tgtgattact cgcggtcagg tgccacctaa cccatctgaa ctgctgatgc gcgatcggat
     7561 gcgtcaatta ctggaatggg cgaacgacca ttacgatctg gtgattgttg atacgccgcc
     7621 gatgctggcg gtgagcgatg ctgcggtcgt ggggcgctct gtcggcacca gcctgctggt
     7681 tgcgcgtttt ggcttgaaca ccgccaaaga ggtgagcttg tcaatgcagc gtctggaaca
     7741 ggctggcgtt aatattaaag gcgcaatcct caatggtgtg attaaacgcg ccagcaccgc
     7801 ttacagttac ggctataact attacggtta tagttactcc gagaaagagt aaaactgaag
     7861 gtaaagtgac cggatgcggc gtgaacgcct tttccggtct acgcattaga cagttcttcg
     7921 tttgtcatca gcctcagagc attcaggtag ctgaatgctc ttttttatac attacaaact
     7981 gcacgccggt atgcgtgctt cattcacgat ttgcgtaaaa ccggccaacg aacacatgcc
     8041 ctgcgcattt cgctcttcac atcctgccag ggtcagtttc acctctccgg gcggcgtatt
     8101 taatgacagc ggagttttat cacgcatctg ctgtaaagtc tggaagacca gcgaaacctg
     8161 aatccactgg ctgttatcgc ttagccgacg ccagcgttca aacaccagtt caccacctgg
     8221 cggcgtgtta tccggctgac ctggaagcgt ccagttgagc tccagtgcgc cgccgagatt
     8281 tgccagatta gtatcgtgtc cggcgataaa cagcactgaa gtgggtaatg tcacaccata
     8341 cgcctgtttt tgcggtggat ggggagtcaa cgctatcatg atcaaatcca acaacggggt
     8401 ggcgcggctg cgggcaacct ctggcgtgcg ttgtagtaaa taaaattgcg cgttatgcaa
     8461 acttagcaac gtgttccact ggtgtgaatc agtgatcctt ccccaccccg gctccggcat
     8521 tccctgtgcc tgttgcagga gaaatatttc cgtcagcatt gatgcgaggc ttaccgcacc
     8581 ggttaatgag acattgtcgg cgctcacctt gagttccgat ggtaatgcct gcgttaatga
     8641 acagctttcg tcctgtttct cacggttaag gcacaagttt gattgcggaa aattaagcac
     8701 ccgttccagt tcgcgaaacg ccgtttgccg atgcccggta aagtcagcaa ttgaccctcc
     8761 tgccctgctg aggatcgcgt cagtcacgtt cgcgttatcc agttggcaaa cgccagtttt
     8821 tagaggatta aataacggat cgggactgga cgtatctgcc tgggtatgta cggttattgc
     8881 acagtcaggt gccagcccgg cggcgaaggc ttcgcctgtt ttacgggtac gctcgtcgac
     8941 atcagcaata atcgcgacct gaccaggctg cgggcagccc tttttcgtca gcaatccgtc
     9001 ggctaccaga cgctggcgtt ggtaatgtcc gagataagcg attagctcac caccgcgcgg
     9061 tgtcagccaa cccagtttta ccggccagtt tggccatgcg tctggggtga catcctgcat
     9121 cagttgcgtg gccttggttg gggcacgcac accatgacga ctgacaatca ccacactttc
     9181 cagcttcagc tccggctccg gctcactctg agcgaatgca gattgcgggg ttaacggaat
     9241 cagaagagat aaaaatggga tcaagatcgc tttcatcgat atgtttccgc ttttcatatc
     9301 cgacattatt gattgcctga tacgacgcta atgcgccgca tcagacttac gttcgttaac
     9361 ttttcaatcg cgggtccagc cataccaaca caagggtgga gagcgaacac atcaacaaaa
     9421 tgccgacgaa ccaaagtaaa taccacattg ttcctgctcc ttagtacagc tcgttttcgt
     9481 tacggcggag agtttctgtc gtcatgcgcc cccacatttt gtagtagctc cagagagtgt
     9541 agagcaacac aatgggcaaa aatatcagca cgattaccaa cataatactc agcgtcagct
     9601 gactggaagt gctgtcccac aacgtcaggc tggagattgg actcacgctt gacggcatga
     9661 caaaggggaa cagcgtgatg cctgccgtga aaatcacgcc aaattgcatc aatgacgcca
     9721 tcaaaaatcc ccaacccggg cggccacgat aaatcgccat caccgtcagc aatgggcaga
     9781 agaaccccag cagcgggaag atccacaaaa cgggcgactc gacaaaatta ttcatccagg
     9841 caccaggtag cactgccacc agtttcatta acggattgga aggaccgtta gcatcctggg
     9901 cgagcagtac aaagccatca ataccgaccc acagccagta acccgccagc aaaaagcaca
     9961 gcatcaccaa cagtgcagcg cgtttagtcg acagctgtga acgcagatga atcacaccaa
    10021 cagttttcag ttgtaaccag acgccacctt gcaaaatcac catcccaagg ctgagcaatc
    10081 cgcacaataa agggaatggc gtcagcagtt gccagaagct gccgagatac tccacgcgta
    10141 attgcggagt gaaggcaaac ggcacgccga gcaacaagtt gccgaaggcg ataccgaaga
    10201 ctaccggcgg caccagactg ccgatgacca gaccggcatc ccacatttta cgccagcgtg
    10261 catcggcgat ttttccgcga taatcaaagg ccagcgggcg gaagaacagc gagcacagca
    10321 ccaggatcat cgccacataa aagccggaaa acgccgctgc atacactctg ggccaggcgg
    10381 caaataatgc cccacctgcg aggatcaacc agacctgatt gccttcccag tgcgcaccaa
    10441 cgctattaat cactatccgg cgttcatcat catcacgcgc caccagcggc agcaaacagc
    10501 caatccccat gtcaaatccg tcggagatca taaagaccac caggatcacg ccaatcagca
    10561 gccaccagat gaagcgcaat gtttcataat caaacatgat tttctccttt acccctgttg
    10621 ctgcgtcggt tgttcactct gcatcgcgct cggccccaga cgggcatatt tctgcatcag
    10681 gtagacttcg gcgattaaga acagggtgta aagccctacg atcatgatca gtgagaaagc
    10741 cagttgtcct gtggttaaag cggagtgcgc ggagtatgtc ggtaagatgt cctgtatcgc
    10801 ccacggctga cgaccaaact cggtcataaa ccacccggct tcaatcgcaa tccacggcaa
    10861 cggcaaactc cagagcgcca ttttcagcac ccagcgatgc tggtcgattt tgccacgcag
    10921 cgtctggaca agcgcaatca gcatcaccag tagcagcagg gaaccacagc ccaccatgat
    10981 gcggaaactc cagaataccg gcgcaacctg aggtatcgcg ccacgcatcg ccgcctggta
    11041 ctgtgcggct gtgacatgat tcatatccgg cgcatagcgg gagagcaaca tgccgtagcc
    11101 caggtcgcct tccagctccc ggaatgcctg caacacatgc ggctcacgat tgccttgcga
    11161 tatttcctgc attaacagcc aggccatacg tccgcgttgc aagcgtgggt aggtttcagc
    11221 catcaaattc ttcagacccg gcacgggttt atctaatgag tgagtggcga ggatccctaa
    11281 cagcacggga attttgatgg caaaggcgtt acgctcttga tcctgttccg gccaggcaac
    11341 cacatggaac ggtgcaggag caggttccgt ttgccactcc ccttccatcg ccgccagttt
    11401 taccggttgt acttgcgcga cttcatacgc agaactgtct ccgagttgca gggtaccgat
    11461 aatcgccaga gtaccgaaga cggaaccgat ggcaaacgag cgtaatgcga catcgcgctc
    11521 ccgtccgcgc agtaaatacc aggcgctgat cgccataata aacatggccc cggtcacgta
    11581 gcccgccatt acggtgtgca caaatttcac ctggctgacc gggttaaaga ccagctcgct
    11641 gaagctggtc atctccatac gcagggtgtc gatatcaaaa tgcgcaccgg tcgggtattg
    11701 catccaaccg ttggcattca atatccacaa tgcagagaga tttgaaccga acgccaccag
    11761 ccacgtcacc agcaggtgct ggtatttatt cagccgctgc cagccgaaga agaaaagccc
    11821 gacaaaggtg gattcgagga agaaggccat taatgcttcc atcgccagcg gtgcgccaaa
    11881 aatatcgccc acatagttgg aatagaatga ccagttagta ccaaactgaa actccatggt
    11941 caggccggta gccacgccaa gagcaaagtt gataccgaag agcttacccc agaagcgcgt
    12001 catatcgcgg tagattgttt tgccggtgac cacgtaaatg gtttccatga tagccagcaa
    12061 aaaaatcagc cccagggtaa ggggtacaaa taaaaagtga tacagcgcgg tcagagcaaa
    12121 ctgccagcgc gataaatcaa tgacatccca catgcgcact cctgtaggcg aagagagaaa
    12181 aataacccgt taatggtaga tctccctctt tatcctgaaa ctctacgacc agaggtattc
    12241 gccgattgcg atattcataa gcctgaaagc gatacttacc gtctttttac gtcggtgcag
    12301 cttccgccag ccactgacat acctcactaa gccgctgcgc agagtcgacc aaatcttctg
    12361 gcgctgccag caccacttcc ggtattgggc agatttcata actttccagt aacgggcctt
    12421 ttaaggtgtc cgtacagcgt aaatgccaga catggcgtaa ccccgtggcg ttgatatagc
    12481 tctcgccata gccaatggta cgaatctgaa tatttcccgg cccacagaga cgtgagagaa
    12541 aaagccgatc agcctcgctg atgggtaatt gcgtcagatt aatgctgtgc ggctgcgcgt
    12601 cggggttacg tacatgcgcc agtaactcat gcgccaacgg taggccattc atcaggccat
    12661 caatgggcgg cggtaacagc gaatctgtcg gcaagagatt ttgcgttgcc gcctgccaca
    12721 acgccagcgg cgcgcagcca gcctccagtt tgtcctccag caacttttcg ccgcgacgtc
    12781 tgcgcacccg ccataatccg cagaagatcg cctcctgtat ttcactttcg ctgtcgtcag
    12841 cctgctgaat gcgcactgac acttcacctt ccccgagaag cgtattgata aagtgacgat
    12901 cgtcggcgtt gagatttaac agatcgactg ccagcacctc gcccaacggc ggctggcgtt
    12961 gacttagttg atgttgtaac tcgtttaaca gcgcaatcac ctgcgggctg tgagcacatt
    13021 gctccagggc cgccatactc ggttcatcat tcacctgaca ggtgatcggc agtggattca
    13081 tgctgaaact gtcatcgttc ggttgcgttc ttggccccag cagatggaaa aaagtttcgc
    13141 tcatgaggca cgctcctgct gtggttcgac aagcccacgc atcaggttta tcagttccgc
    13201 ccacgggtga ataccattca gcacgccgcg atagtttccg ccggtaaaca ctaatgtggc
    13261 gggaaagcga aagacgccaa agcgatctcc gatggcttcg ctctgttcaa ggtcagcaat
    13321 cgccacctgc catgtgtagt cgggaaactc gcgcagtaat tcgccaatca ttaccggatt
    13381 atcgctgacc tctggcgtgc gtttcgggtc actgcttaat aacaccacgc cgtctggcgc
    13441 ttgcgtaagc cagtcgtcaa gacggaattc actaaccggc gtccagccgc gcgccagcat
    13501 tcgttgccac aacgcatcaa atggcgtgtc gttgctcatc cctgctcctc cagtgtcatc
    13561 ctgtactggc gcaagcgaac gccttcgtaa ttttccatcg acagacagtc ataattgaga
    13621 cagcgcgatt cattagccgg ttgcggcaca attccccatg cagccagctg cgccagcgcc
    13681 gcctgttccg ctgcgggcag ttgctcccgt gccagttcgc tcaggctgcc gccgtagtcg
    13741 tcgagcattg cgggttgcag accgacgagg gcaatatgtg ctggcagatg tccgcggata
    13801 tctgccagcg ccagcacttc ggagaaactg ttctggtgca ggctcatttt cttcgcgctg
    13861 agataagccg gaatacgttc tccggcatag gttcgcagcg ttcctggttc cagcccgtag
    13921 tcaatggcat cgagaatcaa cagatggctg gcgctttcga cataccccag caagttcagt
    13981 ccctgagtac cgccatcgac aatctccaca gactcgggcc agtggtaatg ggcatacaac
    14041 cgttccgcca cccgcacgcc gaaaccttca tcggcccaca gcaggttgcc cagccccatt
    14101 accaccacgc gttgctcgct catgaacgct ccttgttact tattttgcca aatttgtggc
    14161 tacggtagcc gttgaccatg gtggagatca ccgtgtcgtc ggacatgatg tcttcacgca
    14221 gcgccatata gacatgaccg atcacaaacg cgccaatcag ccacatcccc agccgatgcc
    14281 agctgtgaat gtccattgag ttgcctcccg tccagtagaa aaattccacc acataacgga
    14341 acggcgcaaa aatagcgtac tggctgtgtt cgctgtacag cgcaaagccg gtgatgatca
    14401 taaagaccga catcaggaaa tagccaaaca tcgccgcctg ggcgatgggg ttatggccta
    14461 tatcggcact cggacgtttt gccagaaaca gataccagcg gatttcatac cacacgccct
    14521 gccaccagct tttacgccat actggcacga taaacagctc gcgggagtat cgattgccaa
    14581 caaaagccca gtagatccgc atcagcaaga ccacggtaaa aaccatcccg gcgctgaagt
    14641 gaattaacct gatgtagccc atatagaaca gatacgtcgc ctcgccgctg acggaaggta
    14701 gcggtttgcc gataaagtaa ccggtgacca tcaacaccgc catgcataac accgtcagcc
    14761 agtgccagat acgcactggc gcttcaaaga catagtggct gacaacgttg tcgcttttct
    14821 gttgcatgat gattctcctt cgctgttaac gcacctgcac ggagatcagc tcgctaccgt
    14881 cgtcgcccag cacgtgtgtt gaacaggcga ggcacggatc aaagctgtgc agagtacgca
    14941 ggatctccag cggttgctcg gggatcgcca ttttggtgtt catcagcgcc gcttcataag
    15001 cgccaatctg ccctttggga tcgcgcgggc tggcgttcca ggtggtcggc accacgcact
    15061 ggtagagatc aatcttgcca tcgcgaatgg cggcccagtg gcctaacgcc ccgcgcggcg
    15121 cttcggtaaa accgacacca cggcactctg tcggccaggt tgtgggttcc catttttccg
    15181 tggaggcggt ggcaagattg ccgtttttca ggttagtcat cagcttgttg aagaaatact
    15241 gcaacttacc tgcggcccac tgtgcttcgt gcgcgcggca caaaatgcgg cccaacgttg
    15301 actggatacc ggaaagcggc aggttcagcg ccgacatcat gcgatcgacc gactcaacgg
    15361 tcgcagcatc gcctttgtga taagcgatta acgtgcgtgc cagcggcccc acttccatcg
    15421 cgttaccgcg ccagcgcggc gctttgatcc acgagtagcg ttcctgttca ttcagctgct
    15481 gaatgttggt atcgctgcct ttgacatcgc cggggttgta ccacgggtcg gtgatgccat
    15541 cgaacggatg acgcccgacc tgatcgttgg gataacgata ccaggcgtga tcgacaaact
    15601 cctgcacctg ctgcggatca accaaatcca ctggcagcac attgttgaag tcgccgttaa
    15661 tcaccgcgcc gccaggcatc agcagacttt tctcaccaaa gtcgttggca atatccggga
    15721 atgcgccgta gctgaggaca catttatcag aaagacccgt gccgatttcg ctccacggtt
    15781 tgttgaactg accgatggct aaggcgtcgg ggatcatcac gttgttaatg aagtctgccg
    15841 tgcgggtgat aatcgactgc accaggttca ggcgttccat attgaccgcc ccgaccgcgc
    15901 cgctttcgtc aatgttaatg gcgcaaggca tcccgccgac aatccagttt ggatgcgggt
    15961 ttttaccgcc aaagaccgcg tggattttga caatttcacg ctggaaatcg agagcttcga
    16021 gatagtgggc aaagcccatc aggttggctt ctggcggcaa cttgtactgc gggtgccccc
    16081 agtagccatt gcggaagatc cccaactgcc cgccttcaac gaatttcttc aggcggtttt
    16141 gtacgtcgaa gaaatagcca ggggatgatt tcggccaaga ggagagactt tgcgccagtt
    16201 cggaggtttt ccgcgggtcg gctttcagcg catccaacac atcgatccag tccatcccgg
    16261 caagctgata gaagtgcacc agatgatcgt ggcaccagag cgttgccagc ataatgttgc
    16321 ggatgatatt ggcgttgtcc ggcactttaa taccgatggc atcttcgatg gcgtaaaccg
    16381 aagccagagc gtgtacgcca gtacagacgc cgcagatacg ttcaacgaac gcccacgcat
    16441 cgcgcgggtc gcgcccttgc aggatgatct ccagcccgcg aaacatggtg ccgcaggaga
    16501 cggcattggt gattacattc tgatcgttaa tattcacttc gcagcgcatg tggccttcaa
    16561 tgcgcgtaat cgggtcgacc accaggcggc gtccggcatt attgatggtg tatccctgag
    16621 tttcgtactg agtgctcatg cctgtttatc ctcattgcct ggctgatgtt cggtttctgt
    16681 aggttgctga ttatgacgtc tgcgctggtc aacggcgctg gcgactgcgt gcacaccaac
    16741 agccgctgcc accacaccaa gcgcggttaa accgacggta tcggcggtgg aatgagtacc
    16801 catttgtgga atatcgacca cgcggctgta gaacgaaccg cgatcccaga aaccattttc
    16861 cgcacagccc aggcagccgt gaccagactg gattgggaaa gaaacgccat cattccagcg
    16921 tgtggaggaa caggcgttat aggtggtagg ccctttgcag cccattttgt acaggcaata
    16981 acctttgcgg gcagcgtcat catcccaact ctggacgaac tctccggcgt cgaagtgagc
    17041 gcggcgatag catttatcgt ggatacgctg accataaaac atcagtggac gacccattct
    17101 gtcgacatct ggcaagcgat caaaggtcac catgtaagta atgatggcgc tcatcacatc
    17161 cgggatcggc gggcagccag gtactttgat aatgggtttg tcggtgatga ctttgtcgat
    17221 agacgttgcc tgtgtcggat taggtcgcgc ggcctgcacg cagccccagg acgcacaggt
    17281 tccccaggcg ataatcgcgc tggctccggc agcggcacgt ttgagtttct caataaacgg
    17341 tcgaccgctg ctgatacaga acatcccctg ctcgcccagc ggcggattac cttctactgc
    17401 gaggatatat ttgccattgt attgcgtgat gatgtcttca aagacttctt ccgcctgggt
    17461 tccggcggca gccatcaaag tatcgtcgta atcgagggaa atcagggaaa ggatgacgtc
    17521 ctttgccagc gggtgagcgg agcggataaa agattcggta cagcaggtgc attccagacc
    17581 gtggatccat accaccggaa tgcgcggttt gttctccagc gcccaggcaa tctttggtgc
    17641 cattcccgcg cctaatccca gcgacgtggc agccagacta caatatttga gaaagctgcg
    17701 ccgggtaacg ccctgacgcc gcatggcctg gtaaaatgtt tcctcgttat tcatatcgca
    17761 cgtctcttct ccttgcgaca ccggcaggac accgggcgct tatggtcggc aagcgacgtc
    17821 cctgtagcaa ggcaaaccat cgcttcacgt gagatgctga aaacgaaagc tcatccttct
    17881 gcactggcgc acgtcgccag aaagtattgt taataaagcg tagtgaaact tttgcacaaa
    17941 acaatacaaa ctgtgtggat ttatctttta gcgataaaaa tggacctatt tttctttttg
    18001 ccgggcggtg ggatgtttag ccagttgcta aacgagtaaa agagaaggaa tttatggtga
    18061 tgaaatgatc aatctgcttt tatttcacgc tatcaatgca cttttggctg tttttcaggc
    18121 aaacaaacag attaggggtt tacacgccgc atcgggatgt ttatagtgcg cgtcattccg
    18181 gaagtgtggc cgagcggttg aaggcaccgg tcttgaaaac cggcgacccg aaagggttcc
    18241 agagttcgaa tctctgcgct tccgccaaat aatcaagggg ttaccaaatg gtagcccctt
    18301 tgcttttttg gctcttggta taaacttggt ataccaattg gtatattttt attcggatga
    18361 gattatcggt catgagcgga acctcaggtt cctcgtctga tgccgctctt gcaacacgtt
    18421 acgctgccga gtatttttgt aaaacatgga ctgctccagg cctgaaccag gcagaaggat
    18481 acaaagccat ctcagatctg tcgcatcact attttcgtgc agaaggctct agccctcccc
//
LOCUS       DBFIFM010000081        18417 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.081, whole genome shotgun sequence.
ACCESSION   DBFIFM010000081 DBFIFM010000000
VERSION     DBFIFM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.081"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..163
                     /locus_tag="N7C74_002421"
     CDS             <1..163
                     /locus_tag="N7C74_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2432275.1"
                     /translation="PYELYNSHRDFLNLKEIKLEHPLPSIKLYISYNKSSLNNLVFSR
                     FIDRLNECF"
     gene            310..1530
                     /gene="ybdN"
                     /locus_tag="N7C74_002422"
     CDS             310..1530
                     /gene="ybdN"
                     /locus_tag="N7C74_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HGU2432276.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1503..2132
                     /gene="ybdM"
                     /locus_tag="N7C74_002423"
     CDS             1503..2132
                     /gene="ybdM"
                     /locus_tag="N7C74_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HGU2432277.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2133..3293)
                     /gene="ybdL"
                     /locus_tag="N7C74_002424"
     CDS             complement(2133..3293)
                     /gene="ybdL"
                     /locus_tag="N7C74_002424"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HGU2432278.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     IADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3402..4490
                     /gene="hcxA"
                     /locus_tag="N7C74_002425"
     CDS             3402..4490
                     /gene="hcxA"
                     /locus_tag="N7C74_002425"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HGU2432279.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGVR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4500..4697)
                     /gene="ybdD"
                     /locus_tag="N7C74_002426"
     CDS             complement(4500..4697)
                     /gene="ybdD"
                     /locus_tag="N7C74_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HGU2432280.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4879..6984)
                     /gene="cstA"
                     /locus_tag="N7C74_002427"
     CDS             complement(4879..6984)
                     /gene="cstA"
                     /locus_tag="N7C74_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HGU2432281.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVVQAKGAH"
     gene            complement(7165..7578)
                     /gene="entH"
                     /locus_tag="N7C74_002428"
     CDS             complement(7165..7578)
                     /gene="entH"
                     /locus_tag="N7C74_002428"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HGU2432282.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDI
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7581..8327)
                     /gene="entA"
                     /locus_tag="N7C74_002429"
     CDS             complement(7581..8327)
                     /gene="entA"
                     /locus_tag="N7C74_002429"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HGU2432283.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8327..9184)
                     /gene="entB"
                     /locus_tag="N7C74_002430"
     CDS             complement(8327..9184)
                     /gene="entB"
                     /locus_tag="N7C74_002430"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HGU2432284.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9198..10808)
                     /gene="entE"
                     /locus_tag="N7C74_002431"
     CDS             complement(9198..10808)
                     /gene="entE"
                     /locus_tag="N7C74_002431"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HGU2432285.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVVYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQINVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10818..11993)
                     /gene="entC"
                     /locus_tag="N7C74_002432"
     CDS             complement(10818..11993)
                     /gene="entC"
                     /locus_tag="N7C74_002432"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HGU2432286.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLY"
     gene            12182..13138
                     /gene="fepB"
                     /locus_tag="N7C74_002433"
     CDS             12182..13138
                     /gene="fepB"
                     /locus_tag="N7C74_002433"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HGU2432287.1"
                     /translation="MRLAPLYRNALLLTGLLFSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSTEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQALLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13142..14392)
                     /gene="entS"
                     /locus_tag="N7C74_002434"
     CDS             complement(13142..14392)
                     /gene="entS"
                     /locus_tag="N7C74_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HGU2432288.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDG"
     gene            14503..15507
                     /gene="fepD"
                     /locus_tag="N7C74_002435"
     CDS             14503..15507
                     /gene="fepD"
                     /locus_tag="N7C74_002435"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HGU2432289.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15504..16496
                     /gene="fepG"
                     /locus_tag="N7C74_002436"
     CDS             15504..16496
                     /gene="fepG"
                     /locus_tag="N7C74_002436"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HGU2432290.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16493..17308
                     /gene="fepC"
                     /locus_tag="N7C74_002437"
     CDS             16493..17308
                     /gene="fepC"
                     /locus_tag="N7C74_002437"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HGU2432291.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTLGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17305..>18417)
                     /gene="wzz(fepE)"
                     /locus_tag="N7C74_002438"
     CDS             complement(17305..>18417)
                     /gene="wzz(fepE)"
                     /locus_tag="N7C74_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HGU2432292.1"
                     /translation="QGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVMAVVFAFA
                     CAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTEAFNLFIK
                     KFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNASKKKDEPS
                     LYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFEKEKLAQD
                     RIKMKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGADGIERKLE
                     IEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKDGPGKAII
                     VILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4353 a         5219 c         4711 g         4134 t
ORIGIN      
        1 cccctatgaa ctttataact cccaccgtga ctttcttaat ttaaaagaga taaaactaga
       61 acatccgtta ccttcaataa agctttacat ttcctataat aaatcttctt taaacaatct
      121 ggtgttctca cgatttattg accgtctaaa tgagtgtttt tgatatataa cctcagacag
      181 ctactataac tttcacctat ttattcacag cgcgaatgaa aaataaaaca ctctgctttt
      241 cagtgtttac tttataatta acagtcttat ctcactgatc accctgtaac gttcagagag
      301 cgtcttcaga tgtctattta taaaattcct cttccgctca atattctgga agctgcacga
      361 gagcgtatta cctggacact caacacccta ccccgcgttt gtgtctcatt ttctggcggc
      421 aaagactctg gtttaatgct gcatctgaca gccgaactcg cccgacaaat gggcaaaaaa
      481 atctgcgttt tgtttatcga ctgggaggcg caattctctt gcaccattaa ctatgttcag
      541 tccctgcgcg agttgtacac cgatgtcatc gaagagtttt actgggttgc gctcccgctt
      601 acgacgcaaa attccctttc acaataccaa cccgaatggc agtgctggga acctgatgtc
      661 gaatgggtac gtcagccccc gcaagatgcg ataaccgatc ctgacttttt ctgcttttac
      721 cagccaggca tgaccttcga acaatttgta cgtgagtttg ccgaatggtt ttcacaaaaa
      781 cgtccggcgg cgatgatgat cggcatccgt gcggatgagt cctacaaccg ttttgtcgcc
      841 atcgccagtt taaataaaca acgttttgcc gacgataaac cctggactac ggccgcacca
      901 ggcggtcata gctggtacat ttaccccatt tacgactgga aagtggctga tatctggacc
      961 tggtatgcaa atcatcaaag tctctgcaac ccactgtata acctaatgta tcaggcaggc
     1021 gttcctctgc gtcatatgcg aatttgcgaa ccttttggcc cggagcaacg acaaggatta
     1081 tggctctatc acgttatcga accggatcgc tgggctgcta tgtgcgcacg agtcagcggg
     1141 gtaaaaagtg gcggcattta cgccggacat gataatcatt tctatgggca tcggaaaatc
     1201 ctcaagccag aacatttaga ctggcaagaa tatgcattat tgctgctcaa tagcatgccg
     1261 gaaaaaacag ctgagcatta ccgcaataaa atcgccattt atttgcactg gtatcagaaa
     1321 aaaggcatcg aggttccaca aacccagcaa ggggacattg gcgcgaaaga tatcccctcc
     1381 tggcggcgga tatgcaaagt tttactcaat aacgattact ggtgtcgggc attatcattt
     1441 agtcctacga aagcgaagaa ctatcagcgt tataacgaac ggataaaagg aaaacgtcag
     1501 gaatggggga tactatgcaa caacgattaa cgcaggattt aacgcaattt ctcgccagct
     1561 tgccggaaga tgatcgcatc aacgcgatta atgaaatccg catggcgatc catcaggtta
     1621 gcccctttcg tgaagagcca gtggattgcg ttctgtgggt taaaaacagc caacttatgc
     1681 ctaatgatta caacccaaac aacgtggcac cacccgaaaa aaaactgctg aaaaaatcca
     1741 ttgaaataga tggttttacc caacccattg tggtcacgca tacggataaa aatgccctgg
     1801 aaatcgtgga tggttttcac cgccatgaaa ttggtaaagg ctcatcatca ttaaaattac
     1861 gcttaaaagg ttatttgccg gtgacctgtc tggaaggtac gcgtaatcaa cgcatcgccg
     1921 cgactattcg tcataatcgc gctcgtggac gccatcaaat caccgccatg tcggaaatcg
     1981 tccgtgaact cagtcagttg ggatgggacg ataataaaat tggcaaagag ctgggaatgg
     2041 acagcgacga agtgctgcgc ctgaagcaaa ttaacggtct gcaagagctg tttgccgacc
     2101 gtcaatattc ccgcgcctgg actgtcaaat aactacagct ggcgcagtcg ttcagctgct
     2161 gccagcaacg tcgattcctt cttggcaaaa cagagacgaa tcagtttatg tgggaaggga
     2221 tcggcgcaaa acaccgatag cggaatcgcc gccacgccgt gctcccgcgt cagccactgg
     2281 caaaactcaa catcatccag ggtagaaacc gcgctgtaat ccaccagcaa aaagtatgtg
     2341 ccttcgcacg gtaaaatctc cagccgactt tcatttaagg catttaccag aatatcgcgc
     2401 ttctggcgat aaaagtccgg taacgcaaga taatgctcag gttctgcacg tagcatatcc
     2461 gcaattgcca gctgtgccgg ggtattcacc gaaaaggtca gatactgatg taccttgcga
     2521 atttcggcgc tgatgggcgc tggcgcaaca caataaccca ctttccagcc ggtcatatga
     2581 taggtcttgc caaacgatga caccgccacc gcccgctcac gcagctgcgg atgcgccagc
     2641 acactggcat ggccctgttg tgaaaagttg atgtgctcgt agacttcatc gctaatgaca
     2701 aaaatctcgt gcccggcgat cgcctgccac aaagcggcga aatcagcctg ctgccagaca
     2761 gttgcactgg gattatgcgg agtgttgagg atcaccagtc tggtgcgctc gcttaacaat
     2821 gcggcaaatt cctgccagtc aacgcgaaaa tgcggtggtt gcagtgccat acgcttcact
     2881 attcccccag aaagcgcgat ggcgggggcg taactgtcat agctgggatc aaaacaaatc
     2941 acttcatcgc cattgcgcac cagtgcggta atcgccgcgt ataacgcttc cgtcgcccct
     3001 gccgttacgg tgatatcgct atcggcatct ggttgatagc catacaaacg ttccgttttc
     3061 tgagcaatcg cctcgcgcaa ggcctgcacg ccggtcatgg gcgcgtattg gtttgccccc
     3121 tgggcaacgt ggtacgccag ccgctcctgt aaatagcgcg gaccatcaaa atcaggaaag
     3181 ccttgcgaca ggttaatcgc ctggtgttgc tgcgccagcg cgctcatctg ggtgaaaata
     3241 gtggtgccaa gttgtggaag tttgctttgt ggaatcagag ggttatttgt catttttatt
     3301 gtacctgacg aagttgcgtg ttgaaggcac tataacacga tgctagtatt tggcaatcaa
     3361 gacgtttaga tgtctaaata aaacaataag gacaacacaa catgcctcac aatcctatcc
     3421 gcgtggtcgt cggcccggct aactactttt cacatccagg aagtttcaat cacctgcacg
     3481 attttttcac tgatgaacaa ctttctcgcg cggtgtggat ctacggcgta cgcgccattg
     3541 ctgcggcgca aaccaaactt ccgccagcgt ttggacttcc aggggcaaag catattttgt
     3601 ttcgtggtca ttgcagcgaa agcgatgtac agcaactggc ggctgagtcc ggtgacgatc
     3661 gcagcgtggt gattggcgtc ggcggcggcg cactactcga caccgcgaaa gccctcgccc
     3721 gccgtctcgg tctgccattt gttgccgctc caacgatcgc cgccacctgc gccgcctgga
     3781 caccgctctc cgtctggtat aacgatgccg gacaggcgct gcattatgag attttcgacg
     3841 acgccaattt tatggtgctg gtggaaccgg agattatcct caatgcgccg caacaatatc
     3901 tgctggcggg gatcggtgat acgctggcga aatggtatga agcggtggtg ctggctccgc
     3961 aaccagaaac gttgccgcta accgtgcgac tggggatcaa taatgctcag gccattcgcg
     4021 acgtcttgtt aaacagtagc gaacaggcgc ttgccgatca gcaaaatcag cagttaacgc
     4081 aatcattttg cgatgtggtg gatgccatta ttgctggtgg tgggatggtc ggtggtctgg
     4141 gcgatcgttt tacgcgtgta gcggcggccc atgccgtaca taacggtctg actgtcctgc
     4201 cacaaaccga gaagtttctc cacggcacca aagtcgccta cggaattctg gtgcaaagcg
     4261 ccttgctggg tcaggatgat gtgctggcgc aattaaccgg ggcgtatcag cgttttcatc
     4321 tgccgactac gctggcggag ctggaagtgg atatcaataa tcaggcggag atcgataaag
     4381 tgattgccca caccttgcgc ccggtggagt ccattcatta cctgccagtc acgctgacac
     4441 cagatacgtt gcgtgcagcg ttcgaaaaag tggaatcgtt taaagcctga cgtgcaaatt
     4501 tagcagcagc gcgcgccgcc ttttccaccg tagcgcgcgt cctgccgctc acggaaaaac
     4561 tcttcgtagg tcatcggcgt ttgatcggga tggttaaccc gcatatgttc gacatagttg
     4621 tcgtaatcag gcataccaat catcagcttc gccgcctgac ctaaatattt tccggctttt
     4681 gccagtgaat caaacatctt ggcctccgct aaccatgttg ctgaaagagt gccggatgcg
     4741 gcgtgaacgc cttatccggc ctacaaatgc tatcaacgcg ctcggtcagc cccctcgccc
     4801 catcggggag agggttaggg tgagggggaa cccacggcgc catgccaaca ttcgccaaca
     4861 tcccccctca ctctgacttt agtgcgcgcc ttttgcctgc accacgatct cctcgacatt
     4921 ttccggcatt ggctcatacg gcgtttcttt cgccgttggc ttcggctctt tcaatgccgc
     4981 aagtgccgtc ttaatcgaga acagtgccag aaccaccacg accaccataa agaagatggt
     5041 taacccggca tccagacggt tgttgaacac cagttgcgcc agctgtgact cagtatactg
     5101 cgatggaata ttaccgctgt cgatcattgc ctggaactta ttagcaatgg ccaggaagcc
     5161 gactttcgca tccgggctaa acgctttctg ccagcctgcg gtcagggtac aaatcagcag
     5221 ccaggccgtt ggtaccagcg ccacccaggc gtaacgttga cgtttcatct tgaacaacac
     5281 cacggcacag agcatcagcg ccatccctgc cagcatctgg ttggcaatac caaacagcgg
     5341 ccacagagtg ttaataccgc ccagcggatc gaccacaccc tgatgcagga agtagcccca
     5401 cgccagcacg cacagcgccg ttgccagcag gttagcaggc agtgaatcgg tccgtttcag
     5461 gcccggagaa accacgccca gcagatcctg caacataaag cgcgcagcac gcgtacctgc
     5521 atccaccgcc gtcagaataa acagagcttc aaacagaatg gcgaagtgat accagaacgc
     5581 cacatccatc atgccgccca gcgcgccatg cagaatgtag gccatcccca ccgccaacgt
     5641 cggcgcaccg cccgcacggg aaatgatcga ctgttctccc acttcgctgg caatctggtt
     5701 gagcgtatcc ggagtaatcg caaagcccca gctactcacc acttgcgcgg cagaagcgac
     5761 cacatccgcc gtccctgccg gagccagcac cgccatcggg ctgttcatgg cgaagtacac
     5821 gcccggatcg atgatacagg cggaaaccag tgccataatc gccacgaagg attccattaa
     5881 cattccaccg tagccgataa aacaagcctg cccttcgttc gccagcatct tcggcgtggt
     5941 gccggaagag atcagcgcat ggaagccaga caccgcgcca caggcgatgg tgataaacag
     6001 gaacgggaac aggttaccgg tccataccgg gccagtgccg tcaacaaatt tggtcaacgc
     6061 aggcatggtc agcgtcgggc gcataatcaa aatgcctacc gccagaccaa cgatcgtccc
     6121 gattttcagg aaggtagaga ggtagtcacg cggagccagc agcaaccata ccggcagcac
     6181 cgccgccaca aaaccgtaac ccaccagcat ccaggtcagc tgcacgccgg taaagtcaaa
     6241 gtacggtgcc caggtcggac tttctgccac ccagccgcca gaaataatgg cgaaaatcag
     6301 gaataccaga ccgatgaccg acacttcacc aatacgcccc ggacgcagat agcgcaggta
     6361 gatccccatg aacagcgcca gcggaatggt gaacgcaacg gtatacgttc cccacgggct
     6421 atgagtcagg gctttcacca cgatcatcgc cagtactgca aggataatga ccatgatcat
     6481 aaagcaggcc accagcgcga tcaccccggc ggttggcccc atctcttctt tgaccagctc
     6541 acccagcgaa cgaccgtcac ggcgcgtaga aacaaacagc accatgaaat cctgcaccgc
     6601 accggcgaga accaccccgg cgagtagcca gatcatccct ggcaggtaac ccatttgcgc
     6661 cgccagtacc ggcccaacca acggacctgc tccggcaatg gccgcaaaat ggtgaccgaa
     6721 cagcactttc ttgtccgtcg gcacatagtc cagcccgtcg ttatggcgca ccgctggcgt
     6781 catacgcgtc gggtcaaccg ccagcacatt tttggcgata tacagaccat aaaaacggta
     6841 agcgatcaga tagatacaga ccgacgccac cacaatccac agcgcgttga tctgttcccc
     6901 acgatttaaa gcaatatatc ccagagcaaa tgctcccatt acagagagca ctgtccagac
     6961 gaggtatttc cctgatttgt tcatagttgt tatccgtgtg cgtgtccata gagatgttac
     7021 attttgtttc tataacatct ttgtacgatt taacaacaca gcaaccatgt aaatcggagt
     7081 tgaaaccaat atttaactta acaatgttaa ctcgatcact ccgttaaccg agttttcctg
     7141 caatctcata taattctgtc gggttcatcc caaaactgcc gtacccagcc gacaagtgca
     7201 gcaacgccgc ccctgttcat cgaaaacgac gatttcccag ctctgatttt gccgcccaag
     7261 atgcagcggc tggcagacgc cgcgtacctt gccttcagac accgggcgat gatgtgtggc
     7321 gttaagttcc gtgcccacca cacactgccc gtcgcgggtc atcataaatc cggccatcga
     7381 tcccagcgtt tcagccagcg ccgccgacgc gccgccatgc agcaggccaa acggctgatg
     7441 agtacggata tcaaccggca tttcggcttc cagcacatca tcgcccagac gggtatacac
     7501 aattcccaga tgcgccacca ttgtgttatc gctggtggcg ttcagttcgt cgagcgttaa
     7561 atggcgtttc cagatcatgc ttatgccccc agcgttgagc cgccatcgac cacaatatcc
     7621 tgtagggtga tatggctggc gaggtcagag gcgaggaaca aaatcgtgtt ggcgatctct
     7681 tgtggacggg cgattttccc cagcggaatg ccgagtttaa actgctcgcc aaagccgcga
     7741 atacgctgtt cttcggcgtc atcgctcacc cacagcgtgc gttgcatatc ggtatcggtg
     7801 gaacccggcg aaaccacatt acagcgcacg ccgctacccg ccagttccag cccgacgctc
     7861 aacgccaggc ttttcagcgc cgctttcgat gcgccataag cactcatacc aatacgcggc
     7921 gtgtgtgcgg cgtcggacgc cacagtgaca atcgccccgc cccgctgacg gcgaaactgg
     7981 ttcatggttt gctggaacag gttaaacgca ccgccgacat taaccgcaaa agtctgctgc
     8041 cagtcctcct tactgagctg atcggtcgcg cccatgcgca aaattcccgc cgcattgacc
     8101 agcacgtcca gtcgctccgt ttcagctaac agtcgctgac atacctgcgc cacctgcgca
     8161 gcatcggcaa catccattac ttcggtcgca aagggatatt gctcctgagc gaacgcttga
     8221 tcaaaacctg taactttcgc tcccgcttca acaaacgcca gcgccgtggc gtagccgata
     8281 cctttccccg cgccggttac ccagacattt ttaccgctga aatccattat ttcacctcgc
     8341 gggagagtag cttccaccag gcgtcgatgg tcgggttttt cgccaacatg acaaagtcga
     8401 tatcgccatg cactttgcgc cagcgcgccg ccagcgccat catgcgcacc gaatccagac
     8461 cgtagtcgat caggttgtcg tcatcgaacg gttcatcgga ctcgtccagc aacggcagga
     8521 tcacctcacg cagcgccgct ttgctggcgg ggataggtgc tggcagtaat tcttcggtca
     8581 tcaccacccg gccagaacgt ccggccacat atttcagcga catcaaatgc tcgtcacggc
     8641 tgaaatcggc cagcgcatcc gccaccataa acggtttaat atcgcgcata aatgcgtcgg
     8701 ttgcggtggt catacagcca atgtgggcat ataccccggt aataatcagc tggttacgtc
     8761 cactctcttt gagcatttgc tccagcggag aacgatgaaa cgcgctgtag cgccacttca
     8821 ccagcaccgt gtcgtcggca tctggcgtca gtcgatccac caccttttgc tgttccggcg
     8881 aacgggtcag gcccggcccc cacatatcat tcaacagcgc ccgatcttca tcgctctgct
     8941 ctttcggctg ggcggtgtaa taaaccggga tattgtgctg tttgcagtag tcgcgcagcg
     9001 cagcaatatt cgcgatcacc tgctccatca tcgggcagtt ctcgccccag aagctgacaa
     9061 aatagtcctg catatcatgg attaacaacg cggcacgttg cggttcaaag gcccagtcaa
     9121 ctttattctg cggaatatcg tgagactccg gcagtgcgta agcctgtaat tttggaatag
     9181 ccatcgtgtt ctctccttca ggctgatgcg cgtgacgcca gccactgacg taattgtttt
     9241 ttatcgactt tcccgaccgc cgtcagcgga agtgaatcca cacactccac gcgatccggt
     9301 aatttaaatt cggcaatacc ctgttcacgc aggaaacgac gcacctgcac cgcgcgaagc
     9361 ggctctttta ccaccagata agcacagctt ttttcgccca tcagctcatc ttccatactg
     9421 accagtgcgg cgtagatcac cgccggatgg cgcagtaaca ggttttcgat ctcttcggca
     9481 gcgatcttct cgccgccacg gttgatctga tctttctcgc gcccctgcac ggtgatgtaa
     9541 ccctctggat caatagagat cagatcgccg gaacagtaaa aaccgttggc atcaaaggcg
     9601 ctggcattgt gctgcgggct tttgtaatag ccacggaagg tatacggccc gcgcgtcatc
     9661 agtcgtccga cttccccttg cggcagtgga tttccttcgg catcggcaac ccatacttcg
     9721 tcatccggac acattgggta accctgggta tggataattt tctccgcgct atcatcaaga
     9781 cgggtatagt tcaccagccc ttccgccata ccaaacacct gctgcaactg gcaaccaatc
     9841 tcagcgggaa tacgcgccgc aagcgtggca gaaagacgtg cgccaccgac ctgtaacagt
     9901 ttcagcgagg caagctgcgc ccggctttcg ccttcggtca gcgcctgcaa ccacaggctg
     9961 actgccggcg gcaccagcgc ggtaacgtta atctgatgtt tttcaatcaa tgggaagcaa
    10021 agcgtggcgc tgggatcggc cgccagaaca accgttcctc cggcaagaaa gacgcccagc
    10081 gatcccggcg aactcatggc gtagttatga gccgccggga tcgcgcacag gtagcgtgtc
    10141 tgttgtgtga actgacaaat ctcgacgcta cgacgcacgc tgtagtagta gtcgttatga
    10201 gtgcgcggga tcagcttcgg tgtaccggtg gtgccgccgg aaagctggaa atagaccact
    10261 tcatcagcag gtgacggcgt agcagtaaaa ccgtctgccg gatggttaat cgcatcctgc
    10321 aagttatgct caccgctgtc gttgagtagc tgcaccacgc gaatggaaga atgctctgca
    10381 acaaatgtgt tgaggaaatc atccccgcta aacagcgcat gttggcgatc ggcaatcagc
    10441 aatgcgggtt caatctggct ggcataggcg ttcagttcac tacgctgatg gctgaacaac
    10501 gccagcaccg gcgcaacgcc cagtttcagc agcgcgaaaa aggtaatgta aagttcagcg
    10561 acgttaccca gttgtaccag cgcggtttca ccaggtttaa tgccctgacg gcgtaaacta
    10621 cacgcgaggt tatccgccgc ctgattcagc tcccggtaac tcaactgtcg ctcgccgtcg
    10681 ataaccgcga tgctgtcact cgcagcgtgg cgagtcagaa tgtcagtcag tggcaaatcc
    10741 tgccagtagc ctttttcccg atagcgacgg gcaaactctt ccggccagcg ggtgaatgga
    10801 atgctcatcc tcgctcctta atacaatcca aaaacgttca acatggtaga aagtttgacg
    10861 cctgtttcgc gccactcacc caacggtgac gacgcaggca caatccccgc tccggcaaac
    10921 agacgcacct gattttcccg cagcttcgcg cagcggatgg tcaccaccca ttcgccgtta
    10981 ccttcgctgt cacaccaacc cacaatgccg ccaaacagtt cgcggtcgaa cggctccagt
    11041 tcagcaataa cctgggtcgc ggcctgatgc gggaaaccgc tcagcgcggg ggtcggatgc
    11101 agcagacagg ccagagtcag tgcgttttct tgcgaattcg ctttaccttc aaagggagtt
    11161 gcgagatgcc acagcgtcgg cgtggtaatc aattgtggag aggaaggaac gtgtaactca
    11221 ctactgcgtt cgcgcagtac ctctttcatc gcctgagtca ccagttcatg ttcatggcga
    11281 tctttttctg acgccagcag acgattaccc gcttcgcgat cgagcacttc atccggctga
    11341 cgacgtgcgg aaccggctaa cggaatggag ctaaaacgct caccgtcttt acgtagcagc
    11401 agttccgggc tggcccccag cagaacgcca ccatctgcca gcgggacatg gaagttgtaa
    11461 ctaaccgggt tttgcgcaat caaccgttcc agcaatacgc cactatcaat ggcggcgtca
    11521 gtggtgatat caatcaaccg tgacaacacc actttgtcga cctgcggcgt ggcggtaagt
    11581 gcggcggcgc gggcaaccat ctgttcaaac gtggtttgct ccggaattgc ctggcgttcc
    11641 accacgttca gcgactggct gcgggtgaaa cggcgtgcgg aagcttgttt ttcctgacgg
    11701 gagaacgact gccaggattc aggaatatac agcgacgaag gctgacgtgg atcgaaagga
    11761 atcgccccga ccatcaccgg atttttgatg ccctgcgctt tggcatcggc aaacagcgcg
    11821 gcgagttttt gctggaaggg actgtcgggc gaatccccgt tcacagccgg ttcatcgaag
    11881 cgggcgaaac atcctgacgt cgtaaaactg cggtacggcg acataaagaa aaagcgattg
    11941 ggcgcaagtg ttgccatggt ctgctgtact tcctcagcca gtgacgtatc catatcatcc
    12001 tccacaaaat gataaaggct ttaataatga ttatcattta tattttcggt cgctaaccta
    12061 aaagcaaacg cgcactatgt caactcttga ggtaacgcaa tctgcaactc ggggttgcat
    12121 ctggttgtgg ttataatgaa aatgagaagc attaacttca ttaataacag gaggctgatt
    12181 tgtgagactc gccccgctct accgcaatgc ccttctatta acaggacttt tgttttcagg
    12241 aatagccgca gttcaggccg ccgactggcc gcgtcagatt actgacagcc gtggcactca
    12301 taccctggaa agccagccgc agcgtattgt ttccaccagc gtcaccctga ccggctcact
    12361 gctggcgatt gatgctccgg tgatcgccag cggcgcgacc acgccgaata accgcgtcgc
    12421 ggatgaccag ggctttttac gccagtggag caaggtggcg aaagaacgca aactgcaacg
    12481 gctctatatc ggcgaaccga gcaccgaagc cgttgccgcg caaatgccgg atctgatttt
    12541 aattagcgca accggcgggg attcggcgct ggcgctgtat gatcagcttt ccaccatcgc
    12601 cccgacatta atcatcaatt acgacgacaa aagctggcag gcgctgttaa cgcaacttgg
    12661 cgaaattacc gggcatgaga aacaagcggc agagcggatt gcgcagtttg ataagcaact
    12721 ggcggcggcg aaagagcaaa tcaaattacc gccgcagccg gtcactgcca ttgtctatac
    12781 tgccgctgca cacagtgcca atctctggac gccggaatca gcacaagggc agatgctgga
    12841 acaactcggc tttacgctgg cgaagttacc cgcagggtta aatgccagcc aaagccaggg
    12901 taaacgccat gacatcattc agcttggtgg ggaaaatctg gctgcagggt taaatggcga
    12961 gtcactattc ctgttcgccg gtgatcagaa agatgccgat gctatttatg ctaatccgct
    13021 gctcgcgcac ctgcctgcag tacaaaacaa gcaggtttat gcgctgggaa ccgagacgtt
    13081 ccgtctggat tactacagcg ccatgcaagt gctggaacga cttaaggcgc tgttttaagc
    13141 attaaccgtc agacgctgtc acctgcggcg gcgtctggcg aaaacgtcgc aactccacca
    13201 gcaccagcaa taacaacacg ccgataatca ataaaccaaa accgctcgcg cttgcggaag
    13261 caaccggtgt catcatcgct cccaaaccac ccagcagcgc cgcgcctatg gcatcgcccg
    13321 tcacgttttg tgccgtccac aaaccgttaa tccgccctaa catcgcttcc ggggtttgcg
    13381 tttgcagcat tgtgtattgc agcaacgagc tgaccgcact caaccagccg aacagcgcca
    13441 gacaaaccac gcctaaaatc cacatcggca tcaggccaaa cagaccaatg gcgaggaacg
    13501 atcccagcgt ggagagcagc atcaataacc ctggtcgcgc gctatgcgcc agcttcccgc
    13561 tggttaacgc gccaatagcc gcgccgagcg ggatcgccgc atagagaaaa ccaatctgtg
    13621 ccgccgacat ctgccagttg tcagccagcg ccggatacag tacccgcacc gcactcgcca
    13681 tcgtcaataa accacccagc agcgcaatcc ctcccaccag cgggctggcg agtagaaaac
    13741 gaaatcctgc cagtaatgat ttcaacggat gctcacgcgg ctgcggtggc ggtggcagtg
    13801 ccggaaggct taacaacggt agcaaggtaa taaacgtgcc cgccgccgcc agcccgtagt
    13861 tccaggccac accgccagtc gccagcagta aaccgccaat catgggcgaa atcaccgacc
    13921 ccagacgcac ggtcaacatg gtgatcgccc cggcctgcat taagttttca cgccctacca
    13981 gtgctggtgt cgccgccaat agcgccgtaa cgccaagcga tgcgaaaaaa ccatcccata
    14041 aaccaagtaa atagattgcc agcaatgacg gctccggcag cagcgcgttc aggcacagtc
    14101 caatgaagcc aatgccacag gtgccgcgcg ccagcaaaat cacttttttg cgctcatagc
    14161 gatccgccag cacaccgccg accatcaggc caacaaacat cgcgccgccg gtcagcgtca
    14221 ccgaaagccc cacctgccag gtggaatgtg tcatcatctg gatctgcacc ggcaccgcga
    14281 cgccgagcaa acccagagac acaattgaga tgaaacgagc gaggaatact gcgcgaaacg
    14341 ccgggtgcgt tttcaacagg ctgaggttaa gcagccagga ttgtttattc attacaatgc
    14401 cttgccatca aatattttaa atacctattc ttaggctgca catgctaaca tatccaaata
    14461 agatcgataa cgataattaa tttcattatc atggaagttc gtatgtctgg ttctgttgcc
    14521 gtgacacgcg ccattgccgt gcccggattg ctgttattac tgattatcgc gacggcatta
    14581 agcctgctca ttggggcaaa atcactcccc gcttccgtag tgctggaggc cttctccggc
    14641 acctgccaga gcgccgactg caccatcgtg ctcgacgcgc ggctgccgcg tacccttgcc
    14701 ggtttactgg caggcggcgc gcttggcctt gccggggcgt taatgcaaac cctcacccga
    14761 aacccacttg ccgaccccgg cttgcttggc gtgaacgccg gagccagctt tgccattgtg
    14821 ctgggtgcgg cgctgtttgg ttactcttcc gcacaggaac aactggcgat ggccttcgcc
    14881 ggggcgctgg tggcctcgtt gattgttgcc tttaccggca gccagggcgg tgggcagtta
    14941 agtccggtgc gtttaaccct ggcgggcgtg gcgctggcgg cggtgctgga agggctgacc
    15001 agcggcatcg ccctgcttaa tcctgacgtc tacgatcaac tgcgtttctg gcaagccggt
    15061 tcgctggata ttcgcaatct acatacctta aaagtggtgc tgatcccagt gctgatcgcc
    15121 ggagcaactg cgctattact gagtcgcgcg ctgaacagtt taagccttgg cagcgacacc
    15181 gcgacggcgc tgggcagtcg cgtggcgcgc acacagttga ttggtctgct ggcgattacc
    15241 gtgctttgtg gtagtgcgac ggcggtagtt ggccctattg cctttattgg cctgatgatg
    15301 ccgcatatgg cgcgttggct ggtgggtgcc gatcatcgct ggtcgctgcc cgtcacgcta
    15361 cttgctaccc ctgccctgct gctgtttgcc gatatcatcg ggcgggtgat tgtgcccggt
    15421 gaactgcgcg tttctgtggt cagtgcgttt attggtgcac cggtgctgat cttcctcgtg
    15481 cgacgtaaaa cgcgaggtgg tgcatgattt acgtctctcg ccgattactc atcacctgtt
    15541 tgctgctgat ttccgcctgt gtggttgcag gtatctgggg attacgcagc ggtgccgtca
    15601 cgctggaaat ctcgcaggta ttcgccgcgc tgatgggcga tgcgccgcgc agtatgacga
    15661 tggtggtcac cgaatggcgt ttaccacgcg tgctgatggc gctgttgatt ggcgcggcac
    15721 tgggcgtcag cggtgcgatt tttcagtcgc tgatgcgtaa cccactcggc agccctgacg
    15781 taatgggctt taacaccggg gcgtggagcg gcgtgttggt ggcgatggtg ctgtttggtc
    15841 aggatctgac ggctatcgcg ctggcagcaa tggtgggcgg cattatcact tcgctgttgg
    15901 tctggctgct cgcctggcgt aacggtatcg acacctttcg gttgattatt atcggtatcg
    15961 gcgttcgcgc catgctggtg gcctttaata cctggctgtt gctgaaagcg tctttagaaa
    16021 cggcgctaac agcaggtttg tggaatgccg gatcgctcaa cggcctgacg tgggcaaaaa
    16081 cctcgccttc cgcacccatc attatattga tgctaattgc cgccgcctta ctggtacgcc
    16141 ggatgcgctt gctggaaatg ggcgatgata ccgcgtgtgc gctgggcgtc agcgtcgaac
    16201 gttcgcgtct gttaatgatg ctggttgcag tggtgcttac cgctgcggca acagcgcttg
    16261 ccgggccgat ttcctttatt gctttagtcg cgccgcacat tgcccgacgc attagcggca
    16321 ccgctcgctg ggggctaacc caggcggcgc tatgcggggc gctgttactg ctggcggccg
    16381 atctctgcgc ccaacaactg tttatgccgt atcaacttcc ggttggcgtc gttaccgtca
    16441 gcctcggcgg tatttacctt atcgttttgt taattcagga gtctcgcaaa aaatgaccga
    16501 atcagtagcc cgtttgcgcg gcgaacagtt aaccctggga tatggcaaat ataccgttgc
    16561 ggaaaatctg actgtagaaa tacctgatgg tcacttcacg gcaattatcg ggccaaatgg
    16621 ctgcggtaaa tccacgttgc tgcgtacctt aagccgcctg atgacgcctg ctcatgggca
    16681 tgtctggctg gatggcgagc acattcaaca ttacgccagt aaagaagttg cccggcggat
    16741 tggtctgttg gcgcaaaatg ccaccacgct gggagatatc accgtgcagg agctggtggc
    16801 gcgtgggcgt tatccacatc aaccgctgtt tacccgttgg cgcaaagaag atgaagaggc
    16861 ggtaacgaaa gcgatgcagg ccacgggaat aactcatctt gcagatcaaa gcgtggatac
    16921 cctttccggt gggcaacgcc agcgagcgtg gatcgcgatg gtgctggccc aggaaacggc
    16981 aattatgctg ctcgacgaac ccacgacctg gctggatatc agtcatcaga ttgatttgct
    17041 ggaactgtta agcgaactga accgcgagaa aggctatacc ctggcggcgg tgctgcacga
    17101 tcttaatcag gcctgtcgtt acgccagcca tttgattgca ttgcgagaag ggaaaattgt
    17161 tgctcaggga gcgccgaagg agattgtcac tgctgaactg attgagcgca tttatggtct
    17221 ccgctgcatg atcattgacg atccggtagc cggaacgccg ctcgtggtgc cgctcggacg
    17281 aacggcacct tcaaccgcaa atagttaaac taagtggtct gccatcatgg catcctgttt
    17341 tctggatgcc atcgcatagc gcaataacac gctaccacaa gccaccatcc cgccgatcag
    17401 cgcggaaagg atcacaataa tcgccttacc cggaccgtct tttttcactg gcaatgacgg
    17461 gcttaactga tatttaaacg gcgtaaaatt cacatcgttg atatttgctt ttgttaactg
    17521 ctcaacaaga tactgacgat tacgtaattc accgttcagt tccgcaacgt cagtgaccgc
    17581 tttttcaatt tccagtttgc gttcaatacc gtctgcgccg agagaaatag agaaatcggg
    17641 gtcatcttta acggcctgac cattactgta aacgggcttt ttaattcctg ccgcgttggc
    17701 aatgtcgagt gaataattga ggcgctgaat gtttgcatca agttgatttt tcattttaat
    17761 gcggtcctga gccagttttt ctttttcaaa ctgggttttg atctccagtt tattacggac
    17821 gttttctatc gactctttca ccaccaacgc agagatataa tcgatatacc cgctcaaaac
    17881 ggtctgcgcc tcttcactgg ttggcgcggt aaaacttagc gtccaggagg tatacagtga
    17941 cggttcatct tttttcttac tggcattgtc atcaaccgct ttcatttttt cgctcaatgc
    18001 gacaattgcg cgatgcaaat ccagttcgtc gattttcgcc tcttttaatt ggtccatcac
    18061 ataaggtgat gaacgcaggt actcttccag caagctaacc gactgaaact tcttgataaa
    18121 caggttaaat gcttctgtac gatcaatttt gatatccaga tccagcacac gaagcttagt
    18181 gaatgttttc tccaactctt gccactgaac aggttctgga ggcgtgacaa ccgccgcgct
    18241 ggtccatttt tgcggcagga tgaaagagat cagcaagcct gcacaggcaa acgcaaaaac
    18301 gaccgccatg accgtttttt tggcccgcca taaaactgag attagattaa gtaaatcaat
    18361 ttcattatta ctgggcgacg ccagaggata atcgggaaaa tgagcgtcac ttccctg
//
LOCUS       DBFIFM010000082        18137 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.082, whole genome shotgun sequence.
ACCESSION   DBFIFM010000082 DBFIFM010000000
VERSION     DBFIFM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.082"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..126
                     /locus_tag="N7C74_002439"
     CDS             <1..126
                     /locus_tag="N7C74_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU2432293.1"
                     /translation="QDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            412..483
                     /locus_tag="N7C74_002440"
                     /pseudo
     CDS             412..483
                     /locus_tag="N7C74_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(494..742)
                     /locus_tag="N7C74_002441"
     CDS             complement(494..742)
                     /locus_tag="N7C74_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGU2432294.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
     gene            complement(970..1035)
                     /locus_tag="N7C74_002442"
                     /pseudo
     CDS             complement(970..1035)
                     /locus_tag="N7C74_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1257..2942
                     /gene="btsS"
                     /locus_tag="N7C74_002443"
     CDS             1257..2942
                     /gene="btsS"
                     /locus_tag="N7C74_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HGU2432295.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYLELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISVRREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            2939..3658
                     /gene="btsR"
                     /locus_tag="N7C74_002444"
                     /gene_synonym="yehT"
     CDS             2939..3658
                     /gene="btsR"
                     /locus_tag="N7C74_002444"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HGU2432296.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            3705..4175
                     /gene="yehS"
                     /locus_tag="N7C74_002445"
     CDS             3705..4175
                     /gene="yehS"
                     /locus_tag="N7C74_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HGU2432297.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(4217..4678)
                     /gene="yehR"
                     /locus_tag="N7C74_002446"
     CDS             complement(4217..4678)
                     /gene="yehR"
                     /locus_tag="N7C74_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HGU2432298.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(4803..6806)
                     /gene="yehQ"
                     /locus_tag="N7C74_002447"
     CDS             complement(4803..6806)
                     /gene="yehQ"
                     /locus_tag="N7C74_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HGU2432299.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATTRPTEKEE
                     EWQPAIWLEELATLPDATRKRAQVLVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEEG
                     NACRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWRMLSAPLRQPGIVALR
                     EYLRQRPPSCIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVASLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPLLQQQG"
     gene            complement(6803..7939)
                     /gene="yehP"
                     /locus_tag="N7C74_002448"
     CDS             complement(6803..7939)
                     /gene="yehP"
                     /locus_tag="N7C74_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HGU2432300.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTPPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSSIPLARDFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSEQWQLV
                     LLVDLSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDMAQALVNVGAQIAAMTPGELATWLAENLQS"
     gene            complement(7932..10211)
                     /gene="yehM"
                     /locus_tag="N7C74_002449"
                     /pseudo
     CDS             complement(7932..10211)
                     /gene="yehM"
                     /locus_tag="N7C74_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            complement(10222..11310)
                     /gene="yehL"
                     /locus_tag="N7C74_002450"
     CDS             complement(10222..11310)
                     /gene="yehL"
                     /locus_tag="N7C74_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HGU2432301.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            complement(11940..12257)
                     /gene="yehK"
                     /locus_tag="N7C74_002451"
     CDS             complement(11940..12257)
                     /gene="yehK"
                     /locus_tag="N7C74_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HGU2432302.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRSTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
     gene            complement(12319..15948)
                     /gene="yehI"
                     /locus_tag="N7C74_002452"
     CDS             complement(12319..15948)
                     /gene="yehI"
                     /locus_tag="N7C74_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HGU2432303.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQQAGQKENITWNYDKCSLEWQSAIQQAINLTGEHKPSIPALTMAALICIAQ
                     NDSQQLLDEIVQQEGLEYATDVVISRQCIARRYESDSLVVTLQYQDEDYGYGYGSATY
                     NDFDLRLRKHLSLAEESCWQRCADKLIAALPGIPKIRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQENRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKAREEYWRTLLQTLMASQPQLAAE
                     VMSWLSTQARAVLKSYLSALPKPVIDGTDNSNLPEILVSPPWRSKKKMTAPRLDLARL
                     ELTPQVYWQPGERERLAATESARYFSTESLAQRMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKSSDKSEAALLALRLLYEHGHGELLQTVANRWQRTDVWPALEHLLKQGPMEIYPA
                     RIPKAPDFWHPQMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQGKSARA
                     VSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRLV
                     PTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPEA
                     LARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWGV
                     YPANEPRRLLNAFRVAAEGGFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLFA
                     DYEIMPPFRQLARRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAFV
                     YDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFNLSEALSAP
                     DVIFH"
     gene            complement(15958..18137)
                     /locus_tag="N7C74_002453"
                     /pseudo
     CDS             complement(15958..18137)
                     /locus_tag="N7C74_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
BASE COUNT         4327 a         4544 c         4697 g         4569 t
ORIGIN      
        1 caggacaggg taatcacgca ggtggtgata ctggatgcgg ataaaaagca gatacagtgc
       61 gtggtaagac cgctgcaaat cctgcgtgct gacgggacgt gggaaaatat tggcgggatg
      121 aagtaacccg acagcttcac aaaaccggag tccggctccg gtttttgtgt tgcaatgtcc
      181 gggggatatt tgttaggtag attgtattgt atgtaaaggc agagctccga attttactgt
      241 tgtaagtggg cctggcggga atggtatcgc cagcatcatt ggcttgggtc cagtaaaaag
      301 agacgcagaa agatgcacaa taggcactac gcgtcatgca tggattaggt cgctcattat
      361 ttacacagta ctgctaaaaa attattaagg aaagcgcggc acttcgtatg taagagcgtg
      421 tcgcggttgg ctggtgaact ttcgatagtg cgagtattga atgatttcca gccgttaccg
      481 atttacgtgt ttattagcga acaaaccact cgtcagcaga ctcccaggta tctttcagcg
      541 tctcctgaac aaaagtttta gctgaatctt tatcggcggt gcgcgtaaca gaaaggccat
      601 cgttgctggt ggcttttacg atcacctcta catcgtcata acgtttactg atgcgtcggg
      661 ttaattcttc ctttaacgca tccacagcac cgtttggcat tttagtgatt ttttctttag
      721 cgatacagat ctcaacacgc ataacaccct cctgtaactg tagttatgta caggtgttat
      781 ttttatctgt atggataacc agtgtcaatt cctgatattg tttatggggc atcaatgggg
      841 catgtatggg acacttttta tcggcgaaat tcgtcgaagt tcatcgacat ggtaaacgaa
      901 tcatctatcc aacccttgaa aaacggcgct cctggacgat cttcgtcgat ttttaaaaat
      961 gttgcgtcac gcgcgtaacg tgacagggtt aatatcacaa agcaacgcca cttcaccaat
     1021 tgtgtaaagc gccatcgtct cacccttgct cgcgaggtcc cggtttaact ttagacgcag
     1081 ttttgcgaac caggtagttt tgcccgtttt ttgtgcattt atagggtgat tttatttttg
     1141 ccaggcgatt ttgagtgatc gtactcacga attctcattt ttctgcaaga gttcaaagaa
     1201 agttaaacgc aggcaatgta tgttacgcgt tttaaaggga tgtgtggttt gcgggtatgt
     1261 acgattttaa tctggtgttg ctgctgcttc agcagatgtg cgttttttta gtcattgcgt
     1321 ggttaatgag taaaacgcca ttattcatac cgttaatgca ggtcacggtt cgtctgccgc
     1381 ataaatttct ctgctacatc gtcttttcca tcttctgcat catgggcacc tggtttgggt
     1441 tgcacattga cgattctatt gccaataccc gtgcgatagg cgcggtaatg ggcggcttac
     1501 tcggcggtcc ggtcgtcggt gggctggttg gcctgaccgg cgggttacat cgatattcga
     1561 tggggggcat gaccgcgcta agttgcatga tctcgaccat cgttgaagga ttgctcggcg
     1621 gcctggtaca cagcatcctg atccgtcgcg ggcgcactga taaagtcttt aaccccatta
     1681 ccgctggtgc cgtcacgttc gtcgctgaaa tggtgcaaat gctgatcatc ctggcgatcg
     1741 cccgacctta tgaagatgcg gtgcgtctgg tgagtaatat tgctgcgcca atgatggtca
     1801 ccaataccgt cggcgcggcg ctgtttatgc gtatattgct cgataaacgc gcgatgtttg
     1861 aaaaatacac ttcggctttt tctgccactg cgctgaaagt ggcagcctcg acggaaggca
     1921 ttttgcgcca ggggtttaac gaagtgaaca gcatgaaagt ggctcaggtg ctgtatctgg
     1981 agctggatat tggtgcagtc gcgattaccg atcgagagaa attgctggcc tttaccggaa
     2041 ttggtgacga ccaccattta cccggcaaac cgatttcttc aacttatact ttaaaagcga
     2101 ttgaaaccgg tgaagtggtc tacgctgatg gcaacgaagt accttatcgt tgctctttgc
     2161 atccgcaatg caaactgggg tcgacgctgg taattccgtt gcgtggtgaa aatcagcggg
     2221 tgatgggcac catcaaattg tatgaagcca aaaaccgttt atttagttca atcaaccgca
     2281 cgctgggcga ggggattgcg caactgcttt cggcgcagat cctcgccggg caatatgagc
     2341 ggcaaaaagc gatgctcacc cagtcagaga tcaaactgct tcacgcccag gtgaaccccc
     2401 attttttgtt taatgcgctt aacaccatta aagcggtgat ccgtcgcgac agcgaacagg
     2461 ccagccagct ggtgcagtat ctttccactt ttttccgcaa aaacttaaag cggccttcgg
     2521 agtttgttac tctcgccgac gaaattgaac atgtgaatgc ttatctgcaa attgaaaagg
     2581 cgcgcttcca gtcgcggttg caggtcaaca ttgctattcc gcaagaatta tcccagcagc
     2641 aattgcccgc gtttaccctg caaccgatag tggaaaacgc cattaaacat gggacatcac
     2701 aactgctgga tacagggcga gtggcaatca gcgtccgacg tgaggggcaa catttgatgc
     2761 tggagatcga agacaatgcc ggtttgtatc aaccggtaac caatgccagt gggctgggga
     2821 tgaatctggt ggataagcgt ttacgtgaac ggtttggcga tgactatggg ataagcgtcg
     2881 cctgtgagcc tgatagttac acccgaataa cgttacgact accatggagg gacgaggcat
     2941 gattaaagtc ttaattgtcg atgatgaacc gttagcacgg gagaacctgc gcgtattttt
     3001 gcaggagcag agcgatattg aaatcgttgg tgagtgttca aacgccgtgg aagggatcgg
     3061 cgcggtgcat aaactgcgcc cggatgtgct gtttctcgat atccagatgc cgcgcatcag
     3121 tggtctggaa atggtgggga tgctcgaccc ggaacatcgt ccgtatattg tttttctcac
     3181 cgcgtttgac gaatacgcta ttaaagcctt tgaagaacat gcctttgact atctgctgaa
     3241 gccaattgat gaagcgcgac tggagaaaac gctggcgcga ttgcgtcagg agcgcagcaa
     3301 gcaggatgtt tcgctgttac cggaaaatca acaggcgctg aaatttatcc cttgtacggg
     3361 gcatagtcgg atttatttgc tgcaaatgaa agatgtggca tttgtcagca gtcggatgag
     3421 cggtgtctac gttaccagcc acgaagggaa agagggcttt accgaattga cattacgtac
     3481 cctggaaagt cgtacaccac tactgcgctg ccatcgtcag tatctggtta acctcgcgca
     3541 tttacaggag attcgtctgg aagataacgg tcaggccgag ttgattttgc gtaatggctt
     3601 aaccgtgccg gtcagccgcc gttatctgaa aagcttaaaa gaggcgattg gcctgtaaaa
     3661 gactgctaaa atggcttttt gcctcatcaa cacctgaagg cctcatgcta agtaacgata
     3721 ttctgcgcag cgtgcgctac attttgaaag ccaataataa tgacctggtg cgtatactgg
     3781 cgctgggtaa tgtcgaagcc accgcggaac agatcgccgt ctggctacgt aaagaagacg
     3841 aagagggttt tcagcgttgt ccggacattg ttttgtcgtc attcctcaat ggcctgattt
     3901 atgaaaaacg cggcaaggat gagtctgctc cggcactgga gccggaacgt cgcattaata
     3961 acaacatcgt gctgaaaaaa ttacgcatcg cgttttcgct gaaaaccgat gacattctgg
     4021 cgatcctcac cgaacagcag ttccgcgttt cgatgccgga aattacggcg atgatgcgtg
     4081 caccggatca taaaaacttc cgcgaatgcg gcgatcaatt tttacgttat tttctgcgtg
     4141 gactggcagc gcgccagcat gtgaagaaaa gctaagacgg gtatggcggc catgcgcaaa
     4201 catggccgcc gacagattat ttcacttctt taaaaccagc ggctttcatc accagttcca
     4261 tttgcgccat agtgatacct tttttggcat cttcagcaga aacgttgatt cctgaaatac
     4321 cctgcagggc tttaaaatcc actttttcca tatcgatagt cacgttttcc tgcgcgtagg
     4381 tatcggtata ggttaatttt tcttcaacac ccgcgatgtt tttgtatttg gcgcttaacg
     4441 gctcaagtgt cttggcagca tcttctttgg tggtagcacc aatggaggca aattgaattt
     4501 tggtttcaga agattgctta agcaccttgt cacctttgta gacataggta atggcaattt
     4561 cagtgccgtt cagattggcg ctgaatttct tcgattcttc tttgtcaccg cagccagcaa
     4621 gagaaaaaac cagaacagat gcaacaacaa gggaaaacag cttattgaaa gccttcatgt
     4681 aaaactccat tttatttaat caaggaactg gtgactctca ccaggggcta tataggatat
     4741 gcctaatacc gtggcgtgag cagtccggaa ctggagtaga actcttagta aaaagcacta
     4801 tttcatcctt gttgctgaag cagggggaat aattgttcgc acagcaaaac accgttattc
     4861 attgcttcta cccgtgcctc gctttctgta ttacgaaatt gtcccaatac atgtgccagc
     4921 cgataaaaac ccaccgcggt gaggtcattc gccagcaact ctgcctgacc aatagcactc
     4981 tgttcctgat agcgccagcc gttatggagc agttgaataa gtaatgcctg gcagcgcatc
     5041 agcaactgat gagcagtaga cggcacaggc agaacgctgg cagaaggtag tgatgccacc
     5101 acaggcgcag tttctgcgtc cagcgcccag gcacgagtct ttgtcatcat tacctgtggt
     5161 tccagtgtca attgcccatc aacaaaactg acaaagccag aaaccagaca cacggggtcg
     5221 tctgtttgtt gcaaaagcgc cgccatgcgt tcaacggcat aaggcgcgct ggctgaggct
     5281 ggcagtgata acgtcagcag attatcttca ccttcgccgc tgattacctg cgcatccagc
     5341 gtctggcggc tgctatccca accgagcgaa atacactcag cgaccggcag aataaataag
     5401 ttatcgacct gattaagagg ccgtatgcag gaggggggac gctggcgtaa atattcccgc
     5461 aaagccacaa tgcccggctg gcgtaacggc gcgctcaaca ttcgccaggc atcaggcgac
     5521 agcggcacaa cgctgcttaa gcggttgcgg gtagctaaca gcagctcgcc atcggcactg
     5581 cgttttgctg cttgtgaaac aatttgccca cccgccagtg cgccagcctg aaaactaaac
     5641 agccgacgcg tagctgccgg tgagttttcc tgttcacttc gcggccaact gcgcgaaagg
     5701 tgcaaaatac tgccggtgtc gggatcggta aaccagatgc gtaaaccata atgctcaata
     5761 tcctgccagc aacgcatacc taaagacacc agccgcagat gatcaagctt tgcttctccg
     5821 gcaatgccag agccaacgac cgtgcgccac ggcacaggag gaacttcacc aacactgtcg
     5881 cgccgggcca tctcttgtgc gcaatttaat cgactgttta atgccgcaag ctgacgtaag
     5941 cattctcctg catgatagtg gctggcgcgg gcgtggaagg catcaacgct ggcgcgcagt
     6001 tgccgtagcg attcactcac ccagcgccag ttgcagcgtt ccgccgcttg ctgcgcgcgg
     6061 ctgaaagcgg cctcgtagtg aataagcggc tggctgatgc cgccaagcca taatgcctgg
     6121 cttaattgct gaacatattg acgacacgcg ttgccttctt cgctggcaaa cgggtcgtca
     6181 gatgatgtga cgtgttcgct gcgcatctgc cagattaaat gagtaaattc cgcttgctga
     6241 gttttggcct cgacgaaggc ctgcacagcc agtacgacat gttcgcaaag tgtgccttca
     6301 atacaatcac agcgggcgaa gcgaatgctg ctgcgggaat aaaaacgcac atcactcatc
     6361 ggtaagcggg ctgagggaat ttcgccaggc gcacagaaca actcaatggt gatgccttta
     6421 gcgaccagca cctgtgcacg tttgcgggtg gcatcgggca gggtagccag ttcttccagc
     6481 cagattgcag gctgccattc ttcttctttt tccgtgggcc gggtggtggc acaaagtcgc
     6541 tgataactta acaccagcat cacgcgatga cggcacatgc cgctagcccc gcaggtgcac
     6601 tgagcctctt tcagtgcctg gccgttcgcc agctgggtac ggacaccgtc actgaaggtg
     6661 gcgattaaag cgccgttctc atggctgatt tccgggacgt tgccattttc cagttcctta
     6721 aggctgcgct taacaaaacc ggcattgctt aacgccgtca gggcctgcgg cgtgagttct
     6781 aataattccg gacgtagtga attcatgact gcaaattctc cgcaagccag gtagccagtt
     6841 cacccggtgt cattgcggct atttgtgcgc caacattaac cagcgcctgg gccatatcgc
     6901 ggtcatagca aggcgttgcg gtgctgtcga gcgctgccag ccccagcact ttgatgccgc
     6961 tctggacaca ctttttcacc tgatgcgtca gcaatgatga tgaaccccct tcataaaaat
     7021 cactcacgag gataatgacg ctttttgctg gttgttcaat aagttgccgc ccatactcca
     7081 cggcactggc gatattggtc ccgccgccca actgtacttt cattaataac tctaccggat
     7141 cggcaacgtc tgccgtgaga tcaacgacac tggtgtcaaa cgccaccaga tgggtacgaa
     7201 tgccgggtaa ctgccacaaa caggccgcca ttaccgcaga gtggatcacc gaatcgacca
     7261 tcgatccgct tagatcaacc agtaagacca gttgccattg ttcactgtgg cgcttaattc
     7321 ggctgttaaa gcggggggat tcgatataca acttgccgtg ttgcgggtgc cagtgttgca
     7381 gattggcgcg aagagtactt ttgaaatcaa agtctcgcgc cagtggaata gatgagcggc
     7441 gacggcgatc gcggacacca gaaaaagcct gacgaacttc ctttgccagt cgagccataa
     7501 tttcttcaac aacctggcgc actatccggc gggcggcagc cagtacttcg gggttcatca
     7561 gatgtttggt gtgcaaaacg gcgcgtagca ggctttcaga aggctgcata cgttccagca
     7621 cgtcgagatt cgtcaccaca tcttcaatgc cgtagcgcag tacggcatcg ctttccagcc
     7681 gctcaatcac ctgttgcgga aacagcgtgt gaatactgtt gatccactca gggggggtga
     7741 gatttgagcc acctaatcca ccggagcgtt caccacgctg gagccgttca ggatcgcgcc
     7801 catacagcca ctccagcgcg tggtctatct gccgggcgtt gtcatccagc ccacaaagcg
     7861 tcgtttctgc cgcttcgcca agaattaatc gccagcgttg tagctcacgg gtggtcagaa
     7921 gatcgttcag ttcagacatg gaaaactccc cagttttgca gcgatgccag tgcctgctgt
     7981 tcgagttgtt gatgatgtgc aatggcttgt ggtgggcaat gcaacggcat ttgcagcgcc
     8041 gaaacgggaa gttgcgccag ttgataatgt tcaagcacct gatgcgccag tgtcccgcgt
     8101 tctcgtggcg gtagccaggc catcgccgcg cgtaaatcgg gcagggcatt gataaaatcg
     8161 gcatcacttg gttgatttaa atgactgctg aaaccggcga taaatgccgg ctgacaggcc
     8221 agttgatggc gggccagcgc cagcaaaccg tgcagcgctt cgccggattg tgccggggat
     8281 aactgcgcca gcatcgtcag cgccgcttcg gcactagcgt tgggatgttc cagacgcatc
     8341 agtgcgccaa gagccgcgcc gcgatccagc gccggggcat ggattgcctg actgcgtcgc
     8401 tccagtaagg ctaccgccgc agaaagagaa acgccgggta aattaacgcc gctatgtaga
     8461 tcgcgcagaa tatggcaaag cgcttgccag ctatgcaggt gagcgtgaaa ctccttttca
     8521 tccggtctgc cgttagattc acacagccac agcgtgcgat cgataaccgc gcataacgtc
     8581 gtttgtgata tctgcgcacc ttgcataccg ctaatttcat ccagccgcca taaggcatat
     8641 agcacttcca gcgccgggcc catttcggca aattgatttt cctaggcgat taataatgtg
     8701 agttgctcca gtaattgttg actgaaggac gccagacccg ctaacgccgc ctggctaaga
     8761 catgtggtga tactgccgat tccacccgcg tccagcatat cggcttctaa tttatggcgt
     8821 gcggcttcct ggagtgtggc accaaagcag gcggcctcaa tcaatgccgc atgctggcta
     8881 agcgggcggg ttaatttcca gcgttcttca ccgttgcctg caagtgtcag tgtgcttccc
     8941 tgctggcgta caatccctgg gatctccagt attgccagcc gatgtaacac ctgactttgc
     9001 gctagtccat tggggttaaa gcgatttaag gtaagctccg ccgaaagaga gattgcggta
     9061 cgttccagtt cgcaggtgac atcttttggc agaggcggtt gtggcgtaga aggggcaagt
     9121 ttaccgaatc cgtcacccgc taatgtgtct atcaacgtta gcagaatcgg atcggtatcg
     9181 ggatgaataa cgccgcgata gctccacggc aacggcgcgt tcagggcttc tttaatcagc
     9241 gagcctgcta tggcatccag ccagtcagtg cgtaacggta gtttatatcc gcgcaactgt
     9301 gccagcgcca tcgcatgcag atgagcggca gccatatccg cggtcgaagc aggcaagtgg
     9361 tgttggcgca aacgggtaag aaccgttttt agcagttgtt ctcccgcctg ctgtaagccc
     9421 cactgccagc accagctttg ccagaccggg gcaggcattc ctgaaaggta tcctgccagc
     9481 acatcaaggc gcttttcact gtagggtgtg agataacaac ctgtaacggc atctgccagc
     9541 gagggcaatt ctggcttgtt aatgtcctgc gggcattcgc gccacatttt tgccagtgcc
     9601 ggagcgtgcc agccaccgca gacgactaac acgtcgccat tattttgctg cactgcccat
     9661 gcaatccagc gagccataaa ggcttcgcgc tgacgattga gtgcatcgcc ggggaaatcg
     9721 ccccgcagtt gggcaaaata gtgcgccagt gcagagggta atgcagtttg ctggctttca
     9781 tcttcgaaca agtgatccca cagggtatcg ctgttatcca tgcgggtggc acgcagcagc
     9841 aaggcctggc tttcctcttg cgtatcaggc gaatcgtcct cttcttcact ctgcgcccag
     9901 caaggcaaat cgataaagta agtttgtgcc tgaatatgac gtgcggcttg tagcgcctgc
     9961 cactccggcg aaaattcggc aaatggcgtc caggcgccgc gcccaggagc agcaccgtcc
    10021 tgatactggc aataactgta aatagccacc ggaagctggt gggctaaaaa cagttcatct
    10081 acccggtcat taaaatcagc cgggccttca atcaacacgt atcgtggtcg ctggctttcg
    10141 attaacgatt tcaccagtcg ggcgcaggcc ggactatgat gccggatgcc gacaattaac
    10201 ggctcgctca tgcatctttc ctcacggcag gcggtggcgg gcttgataat aagcctgcca
    10261 gtgagcttct ttatgtgtgg cgacacgctg ttcgaagtaa cggcgcagac gagcgcgatc
    10321 ttcttcgtta tctttgacga tggttccggc aatacagtcc accaggtcgg caggctcccc
    10381 cgcgcgattc gctaaaaacc aggcgcggac gcccacagcg tgggccacgt tcacggcttc
    10441 agcggtggac ataatcgctg ttagtgtatc cattgacgtt ttcttttcgc cgttggcacg
    10501 cagatcgcgg aaggtgcgga ccagtaactc cagtaccgcg tcaggcactt tatgcggaat
    10561 accgctatgg gccagcaaac gcgccgaagc actggcgaca agctccagct cctgggcgaa
    10621 atccataatc ggaaagacag tttcaaaatc gaagcgacgc tttagcgcag cactcatttc
    10681 attgacgccg cgatcgcggg tattggcagt ggcaataata ttaaagcctt ctcgcgcata
    10741 gagctgactg gcttcaccag tgagttccgg caccgtcatc accctgtcag aaagcatacc
    10801 taacagacag tcttgcactt ccagcggtgt acgcgttatc tcttcaaaac ggacgatttt
    10861 gccgtcgcgc attccctggt aaagcggggc agggaccagc gcttccgttg atggaccatg
    10921 attgataagc agggcgtagt tccagccgta cttgatttga tcttcggtag tagatgcgcc
    10981 gccctggatg gttaacccgg catcgccgct aatagaggtt gccagtaatt cagacaggag
    11041 agattttgcg gtgccaggtt cccccaccaa catcaaaccg cgtccggtgg caagagtgac
    11101 taacatacgt tcgacagcgg agggactaat caccacttta cggctaatat tttgcgcgct
    11161 atcgccaagg ataaaagcac gggccgcagg caaacttaac tgccaaccgg gcgggcaagg
    11221 tgcgttatca ttttgtttta attttgccag ttcatcggcg tataacacag cagcaggcgg
    11281 acgctgcaga tgattattct gcggtgacat atattcatcc ctgaaatgta ttaatgaaat
    11341 aaacgatatt ttacgcgcaa taaatgtttg gcgcattagt tgtttgagcg aaaaatatct
    11401 gggaagcaga tcacagtaaa taaaaaaacc tgctggtgag gcaggttctt ttatttacta
    11461 cagtgacgct aacttgctga ttgtgcaaca tttcgtacca acgaataaat tataaccata
    11521 tccacactcc attgcaattc agtgagtgag atttataatc ctgcccgttg ggaggttgtg
    11581 ccggatcgga ccaattttaa gtcctggctg ccggacgggt ggtgccgcag gcggtgccct
    11641 gatccggaga gcaacgtttc gtaccaacat acgtaaacca gtatcgacag gcagtgtaag
    11701 ggtaaggtgc tggtgttgcg ctccttaata aggagagcgg atggacacgc tgacacaaaa
    11761 gttaaccgtg ctcattgccg tactggagtt attagtggct ctgttacggt tgattgattt
    11821 gttgaagtaa cgggcaaatt gaaacgcatc taagggggaa taccgtttcc ccctttaaga
    11881 aagctatact cgtcatactt caagttgcat gtgctgcgac tttaattatc tagagtatat
    11941 tattctattt cttggttgaa tatattctgt agttcacgat taaaaattgc tattacagtt
    12001 tcaggtgatt cacctcgtaa tttccgctca tcaatacact catctcttat ataaatcaac
    12061 gcatcattta ttagcgggtc agcatcttct cgatcacgtt gatgaaatgt tgaacgtagc
    12121 cggaatcgct ccagtcgata gacggaacct gtaaataatt cagagcattt atggatctca
    12181 agtctgaagg aaaacggatc ttcaggtaca gggacctcat aatcgtaaat agcaaccagc
    12241 tctttttgca cgatcatcgc tttccccgct ggtaagtcgt gttgataacg ggcagttgca
    12301 tcactgcccg ctgtgttttt aatggaaaat gacgtcaggt gcgcttaacg cctcactcaa
    12361 attaaacaca tcaagttcgg caaaagtgac ggatttattg tcacggtaca ctcgaagaga
    12421 acggaagctc attaacgctt tgctatcaac attgtagtgg ttaaaaccgg gtgaaaactt
    12481 aaggaccagc cggtactcac ccaggtcata gacaaatgcg tcctcataac ctgactccca
    12541 gcctttgtat cgcattccca taagttgccc aacggtagcg gatttacctt cccagcgagt
    12601 caggctgtta ctggttgact catcaggtgt gagcagcacc gtgcggcgcg ccaactggcg
    12661 aaaaggcggc ataatttcgt aatcggcaaa aagctgtgca aattcactgc gcatttctgc
    12721 tgtcatttct aacgggtggg caatgccaat cagagcgtcc gcaggcaggt caattggctc
    12781 atcttgcgca ttgcagaacc ccccctctgc ggccacacga aaggcgttga gtaaacgacg
    12841 cggttcattt gccggataaa ccccccatat taatcgctgg gtaaccagac gggtaaaggg
    12901 atgattaaca aacagagaat gaaaatctgc cagcgaccag cgtcgggtgg tacgtagggc
    12961 agcttcaaga cgcgggagca ggtttttgct cacctgagta gcatcttttt ttagcccttt
    13021 tagtcgggcc agtgcctcgg gcgctttcag ttgatcgtca tcggcgcgca accggggaac
    13081 gcttttctgg cgaacgtttt gctgatcaaa gataaccggg ttgaggtttt catcgaagcg
    13141 aacggtaaac tgccggggac caaaatcaaa actcaacgcc tgcggatcat caaggcccag
    13201 ggttgggact aatctgtccg ctagctcttc ctggcttagc ccgcgatttt ctgcgactac
    13261 ctgaagaaat tccgccgcgt tatcacgtaa ggggcgggat ttagcccgtt gcgatatatg
    13321 atgcagctgt atcagcgcca tatcattatt catcagggta aggatgttca ggccgctgac
    13381 agcacgggca gatttgcctt gtggccaggc gaggatctgt gttgttagat cccgtgccgt
    13441 gctttcgtca ccaaagagac ttaacgccag aaatgcccag ttgtcttttg cgggggcacc
    13501 agcttgttgc cacgcagtga agagatccca ggcaaaagct gccagcgttt gtggctggca
    13561 gaacgttttc agttgttcca gcccgctata aaaacgtccc ccctgggtaa agcgcagcat
    13621 ttcgccgata atttccagag cgtcatcggt aacaggttga ttattagtga taaggcgcgg
    13681 cctggaccac atttgcggat gccagaaatc aggggctttt ggaatgcgtg ccgggtaaat
    13741 ttccatggga ccctgtttca gtaaatgctc cagggcaggc catacatctg tacgctgcca
    13801 acgattggct acagtttgta gcaattcgcc atgcccatgt tcgtaaagta aacgcagggc
    13861 aagtaatgca gcttcgcttt tatcgctaga tttggtgaag acaagaggga taagtgcagt
    13921 agccgtatgt tccggccatt gtaaaatcca ctggcgagcc agatcacgct gcgccgcgaa
    13981 acgtcgccag acgcgggcaa cgggcagggc cagttctgtt gcgccgaaat ttaaaattaa
    14041 cggaaatgtt tcttttggac gatgtgaaaa agccaacagc agacctggta gcgcatccaa
    14101 tccgaggata ctgagaaaat agtcctctcc cgcgaacaga tgctttttct cattgatacg
    14161 cagccagaaa gtcaaagcca tctcacgcgg caactgtgcc aataaataca ggttccagac
    14221 actcttttcc ggacgataac gtgagtagtt gtcattttcc catatgttaa ttaacgcttc
    14281 tatgtgaccg ctgcggagcg cctgttctgc taattgtgct tccggggagt tccagccatt
    14341 caggatctct tcaacccgcc cctggtagta atgccactga acaatgagtg aattgcgtgc
    14401 agcatccagt tttccaggga gtatataatt caaaaacagc catacatcat ccccaaaacc
    14461 cagttcctgt aatacaactc gcccactttt ttgttccatg cgttgcgcaa gagattccgt
    14521 gctgaaataa cgggcagact cagtggcggc aagcctctct cgttcgcctg gttgccagta
    14581 aacttgcgga gttaattcga gccgtgccaa atcaagacgt ggagctgtca tttttttctt
    14641 actacgccac ggtggtgaaa cgaggatttc aggcagattg ctgttatcgg tgccatcaat
    14701 aaccggtttt ggcagtgccg ataaataact cttcagcact gccctggctt gagtacttaa
    14761 ccacgacatc acttctgctg ccagttgtgg ctgcgatgcc atcagcgttt gtaacagcgt
    14821 acgccagtat tcttctcgag ctttttgctg tgcaggcagc tttttgtctt cgatgattgg
    14881 ataaccaggg cgggctggtg gttcttttaa cgccagcagt tctgccagtg cggcgagcgt
    14941 cgcatggggg aagtttttac tgtatttagc cacacgttgc aggctgggtt tgtttttatc
    15001 agcaactaac agcaaggtgc ggatgacttg cggatggtta atttgtacca gcagactgcc
    15061 gcaatcttct ttatgagcat acattgccag gcgcggaacg gctgccagtc cttgttcgcg
    15121 caaaagggag gcgcaggccg cgtagccgaa gcggttttct tgtgacatgt agctggcttc
    15181 gcgatcgcta aatacgtcca ggccccagta tcgttcgagt ttttttaccg ccgtattatc
    15241 agtagcaacg acctttaacc actcctttga atggagactt gaccgggagc tttcaagact
    15301 ggcgagttca ttggctattt caggtttttc cgggaggatc agcgcaataa aaggacggcg
    15361 aattttgggt attcctggca gtgcggcaat gagtttatcg gcacaacgct gccagcaact
    15421 ttcctctgcc agcgacagat gcttacgcaa acggagatcg aaatcgttat aggtcgcgga
    15481 gccataaccg tagccatagt cctcgtcctg atactgcaaa gtgacgacca gtgagtcact
    15541 ctcataacgc cgcgcaatgc actgacgcga tatcactaca tccgtcgcat attccagccc
    15601 ctcttgttgc acgatttcat caagtaactg ctggctgtca ttttgcgcga tacagatcag
    15661 agccgccatg gtaagggctg ggattgacgg tttatgctcg ccagtcaaat tgattgcctg
    15721 ctggatggcg gactgccact ccagtgagca tttatcataa ttccaggtga tattttcctt
    15781 ctggcctgct tgttgtagta attcatctcc caatgtctgg atgagacttc cagtgataac
    15841 ggacactggt tcgcccggcc agcgacgatg actgagcggc gttttgcctt ttttatattt
    15901 cagttccagt tgggcgttat ccgccagcca cggtaattcc ttgtccatgc tctttcctta
    15961 ttcaaacagg gcttcaatat cgttaattag ctcgctggcg gtgatggcat cgagtacgga
    16021 gaactgcgct tcctgtgttg aattactccc ccagccatag ctttctgcgt tgccttccca
    16081 tagccacagc ttgcttaaga tttgctcggc actcagttcg gctggcgaca tgcctaccgc
    16141 aaaaccttca tcgatttcca tgattaacga ccagcggccc agaggtttga tcatccagcc
    16201 gatccagcct ccatcctgcg gggtgccctt tatccagcct ttattcgcca gccccatgac
    16261 ccgaccgctc gggcattttc tgcctgccca gcgggtcagt tcgctggcgt tgcgctcggc
    16321 ttctgtcagg gcgtaactgt tacggtcaag ctgacggaac ggtggtagca gttcatagtc
    16381 ggcaaaaagc tgaccaaagg cggtagcatc cgtcggtgat atttccagaa cgtgaggaat
    16441 gccaacagag atatcgcctt ccggcagggt gaaaagatca tcgtcagcgg tgctgtagct
    16501 gttatcttcc gccacgcgga agcacgtcag tagctggttt tcggtgctat aaacgcccca
    16561 aatcagacgg cgggttaagt ggcgtaccag tggatgttca accaggaaga gctgaaagtt
    16621 ttctggcgac cagcggcgac gcaggcacat ggcggattcc aaccttgcta cctgctgggc
    16681 ggcgacggta cgcgcatctt ttttcagcag tttgtagcgg ttaacagcat cgttcgcctg
    16741 tgattcatca tcgcttttgt ttggtttggg caggtctttc agacggctgc cagaaacatc
    16801 acgcacaaac ggttttaagg tttcatcaaa gctgacggtg aactggcgtg agccgaaatc
    16861 cagcagcaac gaaccgttat catccagacc gagatccggt gctaaccgat cttcaagctc
    16921 tgccaccgtg agttcgcggc tttcggcaat gccggcaatt ttttcttttg cccgctcctg
    16981 taatgctttg aatttcagtt tctgggcaat accgttaagc tgcatcaggg cgatatcact
    17041 accgatagcg gcgagaatat ccaacccaac ggtggcgcgt ttatgctggg attcaccagg
    17101 ccaggcgcgt attaatggcg tcagtttgcg ggcggtgtca tcgttaccaa gaacgcctaa
    17161 cgcagtgaac gcccaactct ctttcgacgg tgcgcccgca gtcagccagg cggtaaacag
    17221 atcccaggca aattcagcca gtgagtcggc ggtacaggca tctttcacct gcaataatcc
    17281 cggatacagg gcctcttcct gagggaagcg gagcatttca ccgaggtgct ggagtgcgct
    17341 atctggcagt accttgctgc tatttttgag tttcgggcga gtccataatg gaggttgata
    17401 aaacaacggt aaagggggga ttttcgccgg gtatttaccc acaggactgt tgtcgttatt
    17461 tttattttga gtttcccagg gcggcgagac tagtaccatt ggtagaattt ctgagtttgc
    17521 aaaatcttca ttgcgactat tcagcaattg ctgacattgt tgcaataaat ttgccgcttg
    17581 cgacgataat ccgatgacta atttttcgac aaataaagga tttgcattaa gtaatgaaac
    17641 cagcaatgta tgtttgcgtt tatcatcttt ttttaccaat gcttcggcta atgccactat
    17701 cgttgcagaa ggaaaatttt cggcactttt tattcgtaat ggccaacgga tgattaatac
    17761 atttcagtac gtcaccgcaa ttatcttctt cagcataggg agtcaggcga gccagtgccg
    17821 ctataccttg ctcccgtaat accgtagctg accaactggc accataggtg ttaaatacat
    17881 caatgtattg atagttctcc agtatttcga gcgtgaatgg atctgtcgct accagtttca
    17941 acatttcaag ttgtggaaca tctgcgacct ggtgtaaacg atgtacgatc tcatgggcca
    18001 cttcgggtat ttctggcatt aaaatggcaa ttaacggttg gcgatagagt gatatatctg
    18061 gtaacgcaac gagtaatttt gcaatgcaat tttgccatac ttcttcttct gctagtgata
    18121 aatgtttacg cagacgt
//
LOCUS       DBFIFM010000083        18114 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.083, whole genome shotgun sequence.
ACCESSION   DBFIFM010000083 DBFIFM010000000
VERSION     DBFIFM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.083"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            7..1392
                     /gene="dcuC"
                     /locus_tag="N7C74_002454"
     CDS             7..1392
                     /gene="dcuC"
                     /locus_tag="N7C74_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HGU2432304.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIIEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(1433..2113)
                     /gene="dpiA"
                     /locus_tag="N7C74_002455"
                     /gene_synonym="citB"
     CDS             complement(1433..2113)
                     /gene="dpiA"
                     /locus_tag="N7C74_002455"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HGU2432305.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2082..3740)
                     /gene="dpiB"
                     /locus_tag="N7C74_002456"
     CDS             complement(2082..3740)
                     /gene="dpiB"
                     /locus_tag="N7C74_002456"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HGU2432306.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISEVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            4120..5178
                     /gene="citC"
                     /locus_tag="N7C74_002457"
     CDS             4120..5178
                     /gene="citC"
                     /locus_tag="N7C74_002457"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HGU2432307.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIAMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            5193..5489
                     /gene="citD"
                     /locus_tag="N7C74_002458"
     CDS             5193..5489
                     /gene="citD"
                     /locus_tag="N7C74_002458"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HGU2432308.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            5486..6394
                     /gene="citE"
                     /locus_tag="N7C74_002459"
     CDS             5486..6394
                     /gene="citE"
                     /locus_tag="N7C74_002459"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HGU2432309.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            6405..7937
                     /gene="citF"
                     /locus_tag="N7C74_002460"
     CDS             6405..7937
                     /gene="citF"
                     /locus_tag="N7C74_002460"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HGU2432310.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLILASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTLFLKDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            7941..8492
                     /gene="citX"
                     /locus_tag="N7C74_002461"
     CDS             7941..8492
                     /gene="citX"
                     /locus_tag="N7C74_002461"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HGU2432311.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            8467..9345
                     /gene="citG"
                     /locus_tag="N7C74_002462"
     CDS             8467..9345
                     /gene="citG"
                     /locus_tag="N7C74_002462"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HGU2432312.1"
                     /translation="MSMPATLTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQLLTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            9396..10859
                     /gene="citT"
                     /locus_tag="N7C74_002463"
     CDS             9396..10859
                     /gene="citT"
                     /locus_tag="N7C74_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HGU2432313.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            10973..11779
                     /gene="rna"
                     /locus_tag="N7C74_002464"
     CDS             10973..11779
                     /gene="rna"
                     /locus_tag="N7C74_002464"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HGU2432314.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQVSIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            12009..12419
                     /gene="rnk"
                     /locus_tag="N7C74_002465"
     CDS             12009..12419
                     /gene="rnk"
                     /locus_tag="N7C74_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HGU2432315.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            12557..12646
                     /gene="yldA"
                     /locus_tag="N7C74_002466"
     CDS             12557..12646
                     /gene="yldA"
                     /locus_tag="N7C74_002466"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HGU2432316.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(12650..13888)
                     /gene="ybdR"
                     /locus_tag="N7C74_002467"
     CDS             complement(12650..13888)
                     /gene="ybdR"
                     /locus_tag="N7C74_002467"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HGU2432317.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            14109..14537
                     /gene="uspG"
                     /locus_tag="N7C74_002468"
     CDS             14109..14537
                     /gene="uspG"
                     /locus_tag="N7C74_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HGU2432318.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(14658..16223)
                     /gene="ahpF"
                     /locus_tag="N7C74_002469"
     CDS             complement(14658..16223)
                     /gene="ahpF"
                     /locus_tag="N7C74_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HGU2432319.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(16352..16915)
                     /gene="ahpC"
                     /locus_tag="N7C74_002470"
     CDS             complement(16352..16915)
                     /gene="ahpC"
                     /locus_tag="N7C74_002470"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HGU2432320.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            17287..18033
                     /gene="dsbG"
                     /locus_tag="N7C74_002471"
     CDS             17287..18033
                     /gene="dsbG"
                     /locus_tag="N7C74_002471"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HGU2432321.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         4307 a         4760 c         4480 g         4567 t
ORIGIN      
        1 aaaattatgc tgacattcat tgaactcctt attggggttg tggttattgt gggtgtcgct
       61 cgctacatca ttaaagggta ttccgccact ggtgtgttat ttgtcggtgg cctgttattg
      121 ctgattatca gtgccattat ggggcacaaa gtgttaccgt ccagccaggc ttcaacaggc
      181 tacagcgcca cggatatcat tgaatacgtt aaaatattac taatgagccg cggcggcgac
      241 ctcggcatga tgattatgat gctgtgtgga tttgccgctt acatgaccca tatcggcgcg
      301 aatgatatgg tggtcaagct ggcgtcaaaa ccattgcagt atattaactc cccttacctg
      361 ctgatgattg ccgcctattt tgtcgcctgt ctaatgtctc tggccgtctc ttccgcaacc
      421 ggtctgggtg ttttgctgat ggcaacccta tttccggtga tggtaaacgt tggtatcagt
      481 cgtggcgcag ctgctgccat ttgtgcctcc ccggcggcga ttattctcgc accgacttca
      541 ggggatgtgg tgctggcggc gcaagcttcc gaaatgtcgc tgattgactt cgccttcaaa
      601 acgacgctgc ctatctcaat tgctgcaatt atcggcatgg cgatcgccca cttcttctgg
      661 caacgttatc tggataaaaa agagcacatc tctcatgaaa tgttagatgt cagtgaaatc
      721 accaccactg cccctgcgtt ttatgccatt ttgccgttca cgccgatcat cggtgtactg
      781 atttttgacg gtaaatgggg tccgcaatta cacatcatca ctattctggt gatttgtatg
      841 ctgattgcct ccattctgga gttcctccgc agctttaata cccagaaagt tttctctggt
      901 ctggaagtgg cttatcgcgg gatggcagat gcgtttgcta acgtggtgat gctgctggtt
      961 gccgctgggg tattcgctca ggggcttagc accatcggct ttattcaaag tctgatttct
     1021 atcgctacct cgtttggttc ggcgagtatc atcctgatgc tggtattggt gattctgaca
     1081 atgctggcgg cagtcacgac cggttcaggc aatgcgccgt tttatgcgtt tgttgagatg
     1141 atcccgaaac tggcgcactc ttccggcatt aacccggcgt atttgactat cccgatgctg
     1201 caggcgtcaa acctgggccg taccctttcg cccgtttctg gcgtagtcgt tgcggttgcc
     1261 gggatggcga agatctcgcc gtttgaagtc gtaaaacgca cctcggtacc ggtgcttgtt
     1321 ggtttggtga ttgttatcgt tgctacagag ctgatggtgc caggaacggc agcagcggtc
     1381 acaggcaagt aaatagtaat gccggaggca agtttcctcc ggctttatca tgtcacccac
     1441 tgtggtatat gcgttgcggt ctgccaactt tgccgtgaac aatttcagca ataatcagat
     1501 ggcggctggc gcaatattca agataacgcc tggcagtggt gcgactgatg gtcagtgcct
     1561 gcgccaccgt ttctgccgta tgttgcacac caggctcttt aaacagtttt cgcaccgcgt
     1621 ttagcgtcaa ggggtcaatg ccggtcggca gttcgtcttt gggttcaccg cgagcatagg
     1681 cattaaacat ctcatcaatt tgcttctggc tggcgctatc aatactttcc agcatatgtt
     1741 tacgctggcg gaaacgggtt agcgtttgcc ccagccgttc ataggcaatg ggcttaatga
     1801 gataatcaaa tacaccacaa cgtacggctt cagacaccgt ttccatatcg ctggctgcag
     1861 tggtaaacac cacgtcgccg ggataatgcg cctgcaccag ttcatgcagt aaattaatcc
     1921 ctctaccgtc aggaagatag ttatcgagca agattagccc cggcttaaaa cgctcgatca
     1981 tcattcgggc ctgcgccagg tttcccgcca gtaatatctg actgaatccg ggaatgtgac
     2041 gaatatattc agcatgcatc tctgccagcg gcgtttcgtc ctcaacgatc aatagggtta
     2101 atggagctgt catttggttt cactttcgga atatagattg aaaataaggt accgcaggga
     2161 tcattatctt cgagagtgat aacaccaccg cagcgcgtta cgtagctggc aatcaagtac
     2221 aacccaatgc catgttcacc gggctcgtca gcacgcgtac tgaccccctg ctcaaatatt
     2281 ttgtctcgta gagactctgg aacgccgcag ccctgatcgg cgacttcaat caccacatca
     2341 tcgccttcat cgctgaggaa taattcaacg atcttgtttc cttcatcgct acgcaggctg
     2401 gcttcgaagg cgttatcaag taaattgccc acaatggctg caaactcggt gctatccagt
     2461 cctggcggca gttgcgaaag ctggctaccg gggacaatga tcatttttag ccccagttcc
     2521 cgggcgcgct gcactttacc aaaaagcagc cccgccacct ggcgatcggc aaacgcctcg
     2581 cgcaggctgt caataagctg ttgctgggcc tgagactccc cctgcaccat cgccagcacg
     2641 cgatcatact ctttcatctg caacagacca ttgagcgtcg acatccaatt gagatgctcg
     2701 tgtcgcaatg tacgaaggct ctcaacgtat tgttttattt gcgtcagttg cgcattgagg
     2761 gtggaaattt cgtctttact gcgaaagctg ataatggccc ccagcaaatc atcacctgaa
     2821 cgaatagctt cccgattggc aataacgctc agaccgttaa agttagccac cacatcctga
     2881 cgtttttcat caatctgttc ggtaaagaaa tcggcgggcc tgaccacttc agcaatgggt
     2941 ttacccaacc attgccgtcc gggagagctc agccccagca tctttcttgc gttacgattg
     3001 atggcggtaa tgtaaccatg cggatccacc gcaatcagcc cttcataaac cgaactaaac
     3061 agcgcctctt gctgacggac cacgcgtgcg atttgctttg gctccatgcc catcatttgc
     3121 cgacggatat gcgctgccag gaaccacgac agcaacatca gaatccctaa cagcacgaca
     3181 aacacgcctg ccatcggtaa taaaaactca gcccgccagc tatcgatttt actcaccagg
     3241 tagccaatcg acaccacgcc gatgactttt ccatcgtcat caaagattgg cgttttggcg
     3301 cgcatcgcca tccccattga ccctttcccg gtaatgaagt agctctcccc tttctccagc
     3361 gcgccctgtt tggtgaactg cataggataa ccaattttct ccggattagg atggtaaagg
     3421 cggatcgagt gccggtcccc aatcaccaca taatcaaaat cggtatctct ttgtaatttg
     3481 ttagcgatgg tcgccagccg tttgtagtca cgcgttttca cctcagagat gacactgtca
     3541 ttggaggcaa taattttcgc ctgattcatt gccatgtcgc gtacatgaag cgttaaatag
     3601 tcctcaaaac tggccgtaaa atattgcgcc agggctgcaa tgacaaatat tgagaacacc
     3661 agaatcagca aaaagatacg cagcggaaat gccagtcttt ggaaaaatgc aaactgttta
     3721 ttctcgttaa gctgcaacat tgttttcctt gcgggttgtc ccgagcctct ttgacggatg
     3781 taattaaaat gttgaatcaa aatacataga tattaattat gatcgtggca aattttattc
     3841 attaaatcaa ttaaatcact taaacctgcg agtcatttcg taattacatt aattaaaaaa
     3901 cttaaaacca ttaaataaat aaaaaaccac taactctatg tgaaataaat caaaatttca
     3961 cgccgaaata ctccttagga tgtatagcga aaggagaaaa agatatacct cgatcacccc
     4021 cctttctccc aagtgaaaat aaaaggttat cagtttgcaa cattgaacaa cattcgttgc
     4081 aaatcgataa caacatgcac cttcaggata ctatttatta tgttcggcaa tgatattttc
     4141 acccgcgtaa aacgttcaga aaataaaaaa atggcggaaa tcgcccaatt cctgcatgaa
     4201 aatgatttga gcgttgacac cacagtcgaa gtatttatta ccgtaacccg cgatgaaaag
     4261 cttatcgcgt gcggtggaat tgccggaaat attattaaat gcgttgctat cagtgaatcc
     4321 gttcgcggtg aaggactggc gctgacatta gccactgaac tgataaacct cgcctatgag
     4381 cggcacagca cgcatctgtt tatttatacc aaaaccgaat acgaggcgct gttccgccag
     4441 tgcggttttt ccacgctgac cagcgtaccc ggcgtgatgg tgctgatgga aaacagcgcc
     4501 acgcgactga aacgctatgc cgaatcgctg aaaaaatttc gtcatccagg gaacaagatt
     4561 ggctgcattg cgatgaacgc caatcccttt acgaatggtc accgttatct gattcaacaa
     4621 gctgcggcac agtgcgactg gttgcatctg tttttagtca aagaagattc ttcacgcttc
     4681 ccctatgaag accggctgga tctggtgtta aaaggcaccg ccgatattcc acgcctgact
     4741 gtgcatcgcg gctccgaata catcatctcc cgcgctacgt tcccttgcta cttcattaaa
     4801 gaacagagcg tcattaacca ttgttacacc gaaattgatc tgaagatttt ccgtcagtac
     4861 ctcgctcccg cgctgggtgt aactcaccgc tttgtcggta ctgaaccctt ttgtcgcgtt
     4921 accgcccagt acaaccagga tatgcgctac tggctggaaa cgccgactat ctccgcagcg
     4981 cccatcgaac tggttgaaat tgagcggctg cgttaccagg agatgccgat atccgcttcc
     5041 cgggtacgtc aactgctggc gaaaaacgat ctcacggcta tcgcgccgct ggtccctgca
     5101 gtcacgctgc attatttgca gaacctgctt gagcactccc gccaggacgc ggcagctcgt
     5161 caaaagaccc ccgcatgaga aacaggtgaa aaatgaaaat aaaccagccc gccgttgcag
     5221 gcacccttga gtctggggat gtgatgatac gcatcgcccc actcgatacg caggatatcg
     5281 acctgcaaat caatagcagc gttgagaaac agtttggcga tgcaattcgc accaccattc
     5341 tggacgttct cgcccgctac aacgtgcgcg gcgtacagct gaatgtcgat gataaaggcg
     5401 cactggactg cattttacgt gcacgactgg aagccctgct ggcacgcgcc agcggtatcc
     5461 cggctctgcc atgggaggat tgccaatgat ttccgcttcg ctgcaacaac gtaaaactcg
     5521 cacccgccgc agcatgttgt ttgtgcctgg tgccaatgcc gcgatggtca gcaactcctt
     5581 catctacccg gctgatgccc tgatgtttga cctcgaagac tccgtggcat tacgtgaaaa
     5641 agacaccgcc cgccgcatgg tttatcacgc gctgcaacat ccgctgtatc gcgatattga
     5701 aaccattgtg cgtgtaaacg cgctggattc cgaatggggt gttaacgacc tggaagccgt
     5761 cgttcgcggt ggtgcggacg ttgtacgtct gccgaaaacc gataccgctc aggatgttct
     5821 ggatatcgaa aaagagatcc tgcgtataga aaaagcctgt ggtcgtgaac ccggcagcac
     5881 cggcctgctg gcggcgattg aatctccgct gggcattacc cgcgcagtgg aaatcgctca
     5941 cgcttccgag cgtttgatcg gtatcgccct cggtgcagaa gactatgtgc gcaacctgcg
     6001 tacagaacgc tccccggaag gaactgaact gctgttcgca cgctgttcca ttttgcaggc
     6061 cgcgcgctct gcgggtattc aggcgttcga taccgtctat tccgacgcta acaacgaagc
     6121 cggattcctg caagaagccg cccacatcaa acagctgggc tttgacggca aatcgctgat
     6181 caacccgcgt cagattgatc tgctgcacaa cctctacgca ccgacccaga aagaagtgga
     6241 tcacgcccgc cgcgtcgtag aagccgctga agccgccgct cgcgaaggcc tcggcgtggt
     6301 ttccctgaac ggcaagatgg tggacggtcc ggttatcgat cgtgcccgtc tggtgctctc
     6361 ccgtgcagaa ctttccggca tccgcgaaga ataaggcaat caaaatgacg cagaaaattg
     6421 aacaatctca acgacaagaa cgggtagcgg cctggaatcg tcgcgctgaa tgcgatcttg
     6481 ccgctttcca gaactcacca aagcaaacct accaggctga aaaagcgcgc gatcgcaaac
     6541 tgtgcgccaa cctggaagaa gcgattcgtc gctctggttt acaggacggc atgacggttt
     6601 ccttccatca cgctttccgt ggcggtgacc tgaccgtcaa tatggtgatg gacgtcatcg
     6661 cgaagatggg ctttaaaaac ctgatcctgg cgtccagctc cctgagtgat tgccatgcgc
     6721 cgctggtaga acacattcgc cagggcgtgg ttacccgcat ttatacctcc ggcctgcgtg
     6781 gtccactggc ggaagagatc tcccgtggtc tgctggcgga accggtgcaa atccactctc
     6841 acggcggacg tgtgcatctg gtacagagcg gcgaactgaa tatcgacgtg gctttcctcg
     6901 gcgtcccgtc ctgtgatgaa ttcggtaatg ccaacggcta caccggtaaa gcctgctgcg
     6961 gctccctcgg ctatgcaatg gttgatgccg acaacgcaaa acaggttgtg atgcttaccg
     7021 aagaactgct gccttatccg cataatccgg caagcattga gcaagatcag gttgatttaa
     7081 tcgtcaaagt tgatcgcgtt ggcgatgctg caaaaatcgg cgctggtgct acccgtatga
     7141 ccactaaccc gcgcgaactg cttattgccc gtagcgctgc ggatgtgatt gtcaactcag
     7201 gctacttcaa agaaggtttc tccatgcaaa ccggcaccgg cggcgcatcg ctggcggtaa
     7261 cccttttcct gaaagacaaa atgcgtagcc gcgatattcg cgccgacttc gccctcggtg
     7321 gtattaccgc gacgatggtt gatctgcacg aaaaaggtct gatccgcaaa ctgctggatg
     7381 tacagagctt tgacagccac gctgcgcaat cgctggcccg taaccccaat cacatcgaaa
     7441 tcagcgccaa ccagtacgct aactggggtt cgaaaggtgc atcggttgat cgtctcgacg
     7501 tggtggtact gagcgcgctg gaaattgaca cccagttcaa cgttaacgtg ctgaccggct
     7561 ctgacggcgt actgcgtggt gcttccggtg gtcactgcga taccgcgatt gcctctgcgc
     7621 tttccatcat cgtcgcgccg ctggtacgcg gtcgtattcc gactctggtg gataacgtgc
     7681 tgacctgcat caccccgggc tccagtgtcg atattctggt cacagaccac ggtatcgcag
     7741 ttaacccggc acgtccggaa ctggcagaac gtctgcagga agcgggcatt aaagtggttt
     7801 ccattgagtg gctgcgcgaa cgtgcgcgtc tgctgaccgg tgaaccacag ccgattgaat
     7861 tcaccgaccg cgtcgttgcc gttgtgcgtt accgcgatgg ctcggtgatc gatgttgtgc
     7921 atcaggtgaa ggaataagcc atgcacctgc ttcctgaact cgccagccac catgcggtgt
     7981 caattcccga gctgctcgtc agccgggatg aaaggcaagc acggcaacac gtctggctca
     8041 agcgccatcc tgttccactg gtctccttta ccgtggttgc gcctgggccg attaaagaca
     8101 gtgaggtcac acgccgaatt tttaatcatg gcgtgacagc cttgcgtgcc ttagccgcaa
     8161 aacagggctg gcaaattcag gagcaggctg cactggtttc cgccagcggg ccggagggta
     8221 tgttgagcat tgccgccccg gctcgcgacc tcaagctcgc caccattgag cttgaacata
     8281 gtcatcctct cgggcggtta tgggatatcg atgtcctgac gcccgaaggc gaaattctct
     8341 cccgccgcga ctattcactg ccgcctcgcc gctgcctgtt gtgcgagcaa agcgcagccg
     8401 tctgcgcgcg tggaaaaacc catcaactga ccgatttact caaccgcatg gaggcactgc
     8461 tgaacgatgt cgatgcctgc aacgttaact aaaaccacaa agcttgcgac gtcattaatc
     8521 gatgagtacg ccctgctggg ctggcgcgcc atgctgactg aagtcaatct gtcaccgaaa
     8581 ccaggtctcg tggatcgcat taactgcggt gcgcataaag atatggcgct ggaagatttc
     8641 caccgcagcg cgctggcgat tcagggctgg ctaccccgtt tcattgaatt tggtgcctgt
     8701 agtgcggaaa tggcaccaga agcggtactc cacggattac gcccaattgg tatggcttgc
     8761 gaaggtgata tgttccgcgc caccgcgggc gtaaacacgc ataagggcag cattttttct
     8821 ttagggctac tgtgtgcggc aattggccgt ttgcttcagc tcaaccagtt gctaacgcca
     8881 acaaccgttt gttctacggc ggcaagtttc tgccgtggcc tgaccgatcg cgaactgcgt
     8941 accaataatt cacaactgac ggcaggtcaa cggttgtacc agcagcttgg ccttaccggc
     9001 gcacgcggtg aagccgaagc gggttatcca ctggtgatca accacgcctt gccgcattac
     9061 ctcactctgc tggatcaggg gttagatcct gaactggcat tgctcgatac cttgctccta
     9121 ctgatggcga tcaacggcga taccaacgtt gcttcgcgcg gtggcgaggg gggcctgcgc
     9181 tggctacagc gcgaggcgca aacattattg caaaaagggg gcattcgaac ccccgcagat
     9241 ctcgattatc tccggcagtt cgacagggag tgtatcgaac gaaatctcag tccaggcggc
     9301 agtgccgacc tgctgatcct tacctggttt ttagcacaga tttaattatt taagaacttg
     9361 ataaatttgg aaatattaat tttcggagaa cccgtatgtc tttagcaaaa gataatatat
     9421 ggaaactatt ggccccactg gtggtgatgg gtgtcatgtt tcttatccct gtccccgacg
     9481 gtatgccgcc gcaggcatgg cattacttcg ctgtgtttgt ggcaatgatt gtcggcatga
     9541 tcctcgagcc aattccggca acagcgatca gttttattgc ggttactatt tgcgttattg
     9601 gcagtaatta cctgctcttt gatgccaaag aattagctga cccagcgttt aatgcgcaaa
     9661 aacaggcgct gaaatggggc ctggctggtt tttccagcac tacggtatgg ctggtatttg
     9721 gcgcatttat ttttgcatta gggtatgaag tttccgggtt aggtcgtcgc attgcccttt
     9781 tcctggtgaa attcatgggc aaacgcacgc tgacgttggg ttatgcgatt gtcattatcg
     9841 acattctgct ggcaccgttt acaccgtcca acaccgcgcg taccgggggt acggtttttc
     9901 cggtcattaa aaacctgccg ccgttgttta aatcattccc gaacgatccg tccgcgcgtc
     9961 gtattggcgg ctatttgatg tggatgatgg tcattagtac cagtctgagt tcgtccatgt
    10021 ttgtcaccgg tgcggcacca aacgtgctgg gtctggagtt cgtcagcaaa attgccggta
    10081 tccagattag ctggttgcag tggttcctct gcttcctgcc ggttggggtt atcttgctta
    10141 tcattgcgcc gtggctttcc tacgtgctgt acaaaccgga aatcacacac agtgaagaag
    10201 tggcaacctg ggcgggtgat gaactaaaaa ccatgggtgc gctgacacgc agagagtgga
    10261 cgctgattgg ccttgtattg ctcagcttag gtttgtgggt atttggcagt gaagtcatta
    10321 atgctactgc ggttggtctg ctggcagttt cgctaatgct ggctctgcac gttgtgccgt
    10381 ggaaagacat tacccgctat aacagcgcat ggaacacgct ggtcaacctg gcaactctgg
    10441 ttgtgatggc taacggcctg actcgttctg gttttattga ctggttcgcc ggtaccatga
    10501 gtacgcacct ggaaggattc tcaccaaacg caacggtgat tgtactggtt ctggtgttct
    10561 actttgcaca ctacctgttt gccagcctgt ctgcgcacac cgcaaccatg ctgccggtta
    10621 ttctggccgt cggtaaaggt attccgggcg taccaatgga acaactgtgt atcctgctgg
    10681 tgctgtctat cggtatcatg ggctgtctga cgccgtatgc aaccggtcct ggggtgatta
    10741 tttacggctg tggctatgtg aaatcaaaag attactggcg tcttggcgca atcttcgggg
    10801 tgatttacat ctctatgttg ctgttggttg gctggccgat tctcgccatg tggaactaat
    10861 acgttcgcca aacgctgaat aaatcatcac gcccgccagg ttagcccacc tggcgggctt
    10921 ttttatgatt taatagatag tattcagtgt atgtatgagt tccacaccca ttatgaaagc
    10981 attctggcgt aacgccgcgt tgctcgcggt ttctctgctt cccttctctt ctgccaacgc
    11041 cgtagcgttg caggcaaaac agtatggcga ttttgatcgc tatgtcctgg ccctctcctg
    11101 gcaaaccgga ttttgccaga gtcaacacga tcgaaatcgt aacgaacgag atgaatgtcg
    11161 cctgcaaacc gaaacgacca acaaagctga ttttctgacc gtacatggtc tgtggccagg
    11221 attgcctaaa tcggttgctg cccgtggtgt tgatgaacgc cgctggatgc gcttcggttg
    11281 cgctactcgc ccaatcccga atctaccaga agcgcgcgcc agccggatgt gttcatcgcc
    11341 ggaaaccgga ttatcactgg aaacggccgc taaactaagt gaagtcatgc caggagctgg
    11401 cggacgttcc tgcctggaac gctacgaata tgccaaacac ggtgcctgct ttggttttga
    11461 tccggacgca tacttcggta cgatggtacg cctgaatcaa gagataaaag agagcgaagc
    11521 cggaaaattc cttgcggata attacggtaa aacagtgagc cgccgtgact ttgacgccgc
    11581 ctttgccaaa agctggggaa aagagaacgt gaaagcagtt aagctaacgt gccagggtaa
    11641 ccctgcgtat ttgactgaaa ttcaggtctc gatcaaagct gacgccatca acgctccgct
    11701 ttctgcaaac tcattcttgc cacaaccaca cccaggtaac tgtggcaaaa cctttgtgat
    11761 tgataaagcg ggttattaat cctctgattt acaaaacacg ctaattcgtc tttcataaac
    11821 catgacgatt aagaattata cataatgcgc tgcattgagg aattccctga atgtgacgca
    11881 aatcacttca aagagtgagc aacagtcagt atcatcatgt gagttaaacc ctcgccgcct
    11941 gacggtgagg gttttctttt gggtatgttt atctgcgaca ctcgcagtac cgacgacatg
    12001 gagtaaaaat gtccagacca actatcatca ttaacgacct ggatgccgaa cgcatcgata
    12061 ttctgctgga gcaacccgcc tatgctggtt tgccaatcgc cgacgcgtta aacgcagagt
    12121 tggatcgcgc ccaaatgtgt tcgccagaag agatgccaca cgacgtggtg acaatgaaca
    12181 gccgggttaa attccgcaat cttagcgatg gcgaagtgcg tgtgcgcacg ctggtgtatc
    12241 cggcaaaaat gaccgatagc aatactcagc tttccgttat ggctccggta ggtgccgcac
    12301 tgctgggact gcgcgttggc gattccattc actgggaact tccgggcggc gttgcaaccc
    12361 accttgaagt gctggaactc gaataccagc cagaagctgc tggcgactac ctgctttaat
    12421 cccatctgta caagcgataa ctcgctttgc ccagaggatg cactcgcatc ctctttatgc
    12481 aaaatcagca atttttcctg gttctcactg ttcacttttg ctgcaaaatt attccgtttc
    12541 gtttgtggga ataaccatgg cagaagcatt ttacatcctg ataggatttc tgattatggc
    12601 ggcgattatc gtcatggctg tcctttacct cgaaaaccac tcctgacgct catattgttc
    12661 cccccggcat cgcattcacc agacctgaaa ccgccttctg cgccgcctct gcgctttgtg
    12721 caccgggtac cagaatcacc ttacggcact cctcttcacg cttttcaaag atctcatatc
    12781 cccgggcggc ctcttcaaac ggcatatagt gggtaacaat ttcttctggt ttcagtaatc
    12841 ctttctcaat taacggtaat aattctccca gccatgcgtg aacgtgggtc tgtcccattt
    12901 taaacgtcaa ccctttatca aaggcgtcgc caaacaggaa accgtgaata aatccagcgt
    12961 agacgcccgg tacgctaaca atgccgccac gcctgaccgc cgcaatacac tgacgcaacg
    13021 ctttaccgct gctgccctcc agtttcaggt tagtcagcac cgtttccgtg gtgctgcctt
    13081 tcgcttcaaa accgacggcg tctattactg catccacgcc ccggtgacct gccgtttgtt
    13141 caataattga ctgtgccgga tcgctatctt catcgaaatt aatcgggatc gcgccgtagc
    13201 ggtcagcggc gaaacgcaag cggtagggat gatgatcaac aacaaaaatc tgttccgcac
    13261 cgagcaaccg tgcacaggcg attgtcaaca atcccacagg accggcacca tagacagcaa
    13321 cgcttgaacc ttgttggatc tgcgcatttt ttgctgcctg ccatgccgtt ggcagaatat
    13381 cagaaaggaa aagggcttta tcatctgaaa gcaaaggcgg tactttaaac ggccccacat
    13441 tccctttagg gacacggaca tactccgcct gtccaccagg aacgccacca tacaggtgac
    13501 tataaccaaa caatgccgcg ggagctggta tctgtttttt attgagcgca gcgcctttac
    13561 ccgcattggt attttcgcag gcagcatatt gctgcagtcg acagaaaaaa cagtcgccac
    13621 aagcaatgac gaacggaatt accacccggt cgcctttttg caaatttttt acatcctttc
    13681 cggtttcaac tacttccccc ataaattcat gaccaaaaat atcgccatgt ttaacctggg
    13741 gtattttgcc tcgataaaga tggaggtcag agccacagat cgccgttgcc gttatacgca
    13801 gaataatatc atctgcctgt tcaatgcccg gatcgggaac attttctacc tgaacgtgat
    13861 gtgggccgtg ataagtcaat gctttcatgc aacctccaga gacagttcaa aaaagagtca
    13921 gtctttaagg gtagctatca cagcgggaaa acgggcggta ccgcaggttt tgggagaatt
    13981 cctggtgttc agtgagaata tattcatcac agaaacagtt cgtaaggaaa aagaaccaga
    14041 caaatatcag ggataaatat tataatcatg gcgaatgatt tgttcatgat taacagggag
    14101 aaagggttat gtataagaca atcattatgc cagttgatgt atttgaaatg gaattgagcg
    14161 acaaagctgt tcgccacgct gaattcctcg cccaggatga cggagttatt catctacttc
    14221 acgtactacc cgggtcagcc agcctgagcc tgcaccgttt tgccgctgat gtgcgtcgtt
    14281 ttgaagagca tctgcaacat gaagcagaag aacgtttgca aacgatggtc agccacttca
    14341 ccatcgatcc ttcccgcatt aaacaacatg tccgttttgg tagcgtgcgg gatgaagtca
    14401 atgagttagc agaagaactg ggggctgatg ttgtagttat tggttctcgc aacccatcga
    14461 tttcgaccca tctgttaggt tctaacgcct cgagcgtgat ccgccacgcc aatctgcctg
    14521 tactggtcgt tcgctaacag ataaaagtat ttatgccgcc ctgcctggaa catcccggca
    14581 gggcttttta atgccataaa aaagccgcca ggtttgaccc cggcggctaa gcaattgcag
    14641 gtgaatctta cttcttctta tgcagttttg gtgcgaatca ggtagtcaaa agcactcaga
    14701 gaggctttgg caccttcgcc agtggcgatg atgatctgct tgtacggaac cgtcgtacag
    14761 tcacccgctg cgaacacgcc tttcacgttg gtttcgcatt tcgcatcaat gataatctcg
    14821 cccatgcggt tacgttcgac tgcgccttcg agccagttgg tgttcggcag cagaccaatc
    14881 tgcacgaaaa taccggccag ttcgatgttg tgaatatcgc cgctgacacg atctcgatat
    14941 tccagaccaa cgactttgct gccgtcgcct ttcacttccg tggtttgcgc attcagaata
    15001 atgtcgacgt ttttcaggct gcgcagtttg tcctgcagaa cctggtcggc tttcatttct
    15061 ggcgcaaatt ccagcagcgt tacgtgctca acgatacccg ccaggtcaat tgccgcttcc
    15121 acgccggagt taccgccgcc gataaccgct acgcgtttac ctttaaacag cgggccgtcg
    15181 cagtgcgggc agtaggtcac gcctttggtg cgatactgat cttcgcccgg aacgttcatg
    15241 ttgcgccatt ttgcaccggt cgccacgata atgctgcgtg ctttcagtac cgcgccagaa
    15301 gctgtttcaa tctgatgcag accaccttca actgctgctg ggatcagttt gctggcgctc
    15361 tggctgtcga tcacatcaac gtcgtattca tcaacgtgaa ctttcagtgc gcctgccagc
    15421 ttctgccctt cagtcttcgg tacagaaatg tagttttcga tatcaacggt atcgaggatc
    15481 tgaccaccaa aacgttcgcc catcagaccg gtacggatgc ctttacgtgc ggagtaaatt
    15541 gctgccgctg cacccgccgg gccggaaccg acgattaata cgtcataagc atcacgcttg
    15601 ttcagctctt ctgccgcacg tttttccgcg cccgtatcaa ttttggcaac gatttcagtc
    15661 aaagtcatgc ggccctgacc aaactctttc ccgttcacga acactgccgg aacgcccatc
    15721 acgttacgat cagtgatctc gttctggaag gtgccgccgt caattgcagt gtgcttgatg
    15781 cgcgggttca atacgctcat caggttcagc gcctgtacca cgtccgggca gttatggcaa
    15841 gagagcgagt aataggtttc gaattcaaaa tcaccgtcaa tatggcgaat ctgctccagc
    15901 agagactgcg cttctttcga cggatgacca ccggtccaca gtaacgccag taccagcgag
    15961 gtgaactcgt ggcccagcgg ggagcctgca aaacgtggcc cctggttaga acctgggttg
    16021 gtgatcagga aagacggctt acgcaccggc aagctgttat cttctttaaa ggtgactttg
    16081 tctgacagtt ctgcgatttc agccaacagt tccttgattt ctgccgattt agcgctgtca
    16141 tccagcgtgg caattaactc aacaggcttg gtcaatttct caaggtaagc cttgagttga
    16201 gttttcatat ttgtgtcgag catgtttatc tcctgggctt taaacatcat catgcaagcg
    16261 gcctctgcgg ctacctggat gcagcttgca tcatggtgcg gggaagaaaa tcgggtgccg
    16321 cagcacccga ttaattacgc gattgcggaa cttagatttt accaaccagg tccagagacg
    16381 gagccagagt tgcttcacct tctttccatt tagccgggca aacttcacct gggtgagaag
    16441 ctacgtactg tgctgctttg attttacgca gcaggtcaga cgcgtcacgg ccaatgcctt
    16501 cagcggtaac ttcgattgcc tggatgatac cctgcgggtc aacaacgaag gtcgcacggt
    16561 cagccagacc ttcatcttca cgcatgttgt cgaagttacg ggtcagggcg ccagtcgggt
    16621 cgccgatcat cgcatatttg attttagcga tggtttcaga gctgctgtgc catgctttgt
    16681 gggtgaagtg agtatcggta gatactgcgt atacgtctac gcccagtttc tgcagttctt
    16741 cgtagtggtc agcaacgtca cccagttcgg tcgggcatac gaaagtaaag tcagccgggt
    16801 agaagaagaa gacgctccag cggccttcgg tatctttttc ggtgatttcg atgaattcgc
    16861 cgtttttgaa tgcctggttt ttaaaaggtt taattttggt gttaatcaag gacatctata
    16921 cttcctccgt gttttcgatg agatgtaagg taaccaattt ttaccgattc ggctaatgcg
    16981 tttccattat caaatcgata agttttacct tacaaccttc gtaagacaac tttgtgaact
    17041 ttgcaccgcc agcggcgata aaaagtaaaa tggccccctt tccaggtgac cgtgtaatct
    17101 gaactaccct ttggtatctt cagcgccagt ctgcaggctg cgttgttgct cttacaaatg
    17161 caacaacgac atggattaca acaccctcat aaacaaaggg caatcacctg atctaagctc
    17221 ttacctatga caatgatagg ttatgccttt tactcgactt ttgcactgac tgaaaaggac
    17281 aaattaatgt taaaaaagat acttttactg gctctgcttc ctgcaatcgc cttcgcagag
    17341 gaacttcctg ctccagtaaa agcgattgaa aaacagggca ttacaatcat caaaacgttc
    17401 gatgcccccg gaggaatgaa aggttatctc ggaaagtatc aggatatggg cgtcaccatc
    17461 tacctgactc cagatggtaa gcacgctatc tctggttaca tgtacaacga gaaaggtgaa
    17521 aacctgagta acacacttat cgaaaaagaa atttacgcac cagccggacg cgaaatgtgg
    17581 caacggatgg aacaatccca ctggctcctc gacggtaaaa aagatgcgcc ggtcattgtc
    17641 tacgtcttcg ccgatccgtt ctgcccatat tgtaaacagt tctggcagca ggcgcgcccg
    17701 tgggtagatt ctggcaaagt acagttgaga acattgttgg ttggggttat caaaccagaa
    17761 agtccggcga cagccgcggc aattcttgcc tccaaagatc ccgcaaaaac ctggcaagaa
    17821 tatgaagcct ctggtggcaa gcttaagcta aacgtgcctg caaacgtaag tacagagcaa
    17881 atgaaagtgt taagcgacaa cgagaaactc atggacgatc tgggggcaaa tgtcactccg
    17941 gctatctatt acatgagtaa ggaaaatacg ctgcaacagg ccgtggggtt gcccgatcag
    18001 aaaacgctta atatcattat gggaaataaa taaaaggtaa ttcatggatg gttgaactat
    18061 atccctgctt ttcaaccatc ctaaaaaagc atgaattgct cgctctgccc taag
//
LOCUS       DBFIFM010000084        18111 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.084, whole genome shotgun sequence.
ACCESSION   DBFIFM010000084 DBFIFM010000000
VERSION     DBFIFM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.084"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..1380
                     /gene="bcsE"
                     /locus_tag="N7C74_002472"
     CDS             <1..1380
                     /gene="bcsE"
                     /locus_tag="N7C74_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HGU2432322.1"
                     /translation="PAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCSIDPHNY
                     LFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSLLLEEYR
                     SLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAEIQPRSD
                     EKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQIEPLARS
                     IHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTMIESVQG
                     QKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGVLVALRP
                     VPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLPTGDIFS
                     NRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRIPEPMRL
                     LDDAVERSS"
     gene            1377..1568
                     /gene="bcsF"
                     /locus_tag="N7C74_002473"
     CDS             1377..1568
                     /gene="bcsF"
                     /locus_tag="N7C74_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HGU2432323.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            1565..3244
                     /gene="bcsG"
                     /locus_tag="N7C74_002474"
     CDS             1565..3244
                     /gene="bcsG"
                     /locus_tag="N7C74_002474"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HGU2432324.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(3331..3438)
                     /gene="ldrD"
                     /locus_tag="N7C74_002475"
     CDS             complement(3331..3438)
                     /gene="ldrD"
                     /locus_tag="N7C74_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU2432325.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            3914..5185
                     /gene="yhjV"
                     /locus_tag="N7C74_002476"
     CDS             3914..5185
                     /gene="yhjV"
                     /locus_tag="N7C74_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HGU2432326.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFTMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKTWLILLYGILLCLSPLLKLIE"
     gene            complement(5215..6219)
                     /gene="dppF"
                     /locus_tag="N7C74_002477"
     CDS             complement(5215..6219)
                     /gene="dppF"
                     /locus_tag="N7C74_002477"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HGU2432327.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKIGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(6216..7199)
                     /gene="dppD"
                     /locus_tag="N7C74_002478"
     CDS             complement(6216..7199)
                     /gene="dppD"
                     /locus_tag="N7C74_002478"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HGU2432328.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(7210..8112)
                     /gene="dppC"
                     /locus_tag="N7C74_002479"
     CDS             complement(7210..8112)
                     /gene="dppC"
                     /locus_tag="N7C74_002479"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HGU2432329.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(8122..9141)
                     /gene="dppB"
                     /locus_tag="N7C74_002480"
     CDS             complement(8122..9141)
                     /gene="dppB"
                     /locus_tag="N7C74_002480"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HGU2432330.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(9292..10899)
                     /gene="dppA"
                     /locus_tag="N7C74_002481"
     CDS             complement(9292..10899)
                     /gene="dppA"
                     /locus_tag="N7C74_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HGU2432331.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(11644..11720)
                     /locus_tag="N7C74_002482"
     tRNA            complement(11644..11720)
                     /locus_tag="N7C74_002482"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11684..11686),aa:Pro,seq:cgg)
     gene            complement(11812..13503)
                     /gene="eptB"
                     /locus_tag="N7C74_002483"
     CDS             complement(11812..13503)
                     /gene="eptB"
                     /locus_tag="N7C74_002483"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HGU2432332.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVNDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKAADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            complement(13758..14288)
                     /gene="lpfE"
                     /locus_tag="N7C74_002484"
     CDS             complement(13758..14288)
                     /gene="lpfE"
                     /locus_tag="N7C74_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HGU2432333.1"
                     /translation="MKFKRLLHSGIASLSLVACGVNAATDLGPAGDIHFSITITTKAC
                     EMEKSDLEVDMGTMTLQKPAAVGTVLSKKDFTIELKECDGISKATVEMDSQSDSDDDS
                     MFALEAGGATGVALKIEDDKGTQQVPKGSSGTPIEWAIDGETTSLHYQASYVVVNTQA
                     TGGTANALVNFSITYE"
     gene            complement(14293..15348)
                     /locus_tag="N7C74_002485"
     CDS             complement(14293..15348)
                     /locus_tag="N7C74_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2432334.1"
                     /translation="MKAAIALSLLGCVFGFSGKAFAGDAWGPCTPADGTTYHYNVDVD
                     VGIPDAAKNVAGTVLPDVLNWSNGQNVSLICECPDSYKNEKDTLVQGVSMLPPSGRTV
                     DSMKYYTLTEELEVATNIRISTSVYGFVPFKNQQALQTTGCNKVITTPYMGGAGLLSF
                     AITKPFIGDSVIPLTLIAELYASKTNKDYGTIPISSVSIQGRVTVTQDCEIKPGTVLD
                     VPFGEFPSSAFKNRQGQMPEGATEQEINLSFDCNNISDGIKVALRLEGATNADDPRAV
                     DMGNPDIGVLVKDSSGKILVPNDSSSTTLLNLSSLDSKTHRNAAIRLLALPISTTGKA
                     PKGGTFEGVTTIYLEME"
     gene            complement(15363..17936)
                     /gene="lpfC"
                     /locus_tag="N7C74_002486"
                     /pseudo
     CDS             complement(15363..17936)
                     /gene="lpfC"
                     /locus_tag="N7C74_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088424.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
     gene            complement(17965..>18111)
                     /locus_tag="N7C74_002487"
     CDS             complement(17965..>18111)
                     /locus_tag="N7C74_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312457.3"
                     /note="binds with fimbrial subunit LpfA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB"
                     /protein_id="HGU2432335.1"
                     /translation="EIEKAGMVPAKGALNITLPAGVGSVSKIKYNTINDFGSSAEMIT
                     KNVD"
BASE COUNT         4319 a         4843 c         4465 g         4484 t
ORIGIN      
        1 ccggcgaaaa tctttcaatt agatgattct caagggccgg aaaaaataaa attattttca
       61 atgctaaatc atgaaaaagg tctatactat ttggcccgtg atttgcagtg ttctattgat
      121 ccccataatt acctttttat tcttgtttgc gcaaataacg catggcaaaa cattcctgcc
      181 gagcggctgc gctcatggtt ggataaaatg aataaatgga gcaggttaaa ccattgttcg
      241 cttttggtaa ttaatcccgg aaataataac gataaacaat tttcattgtt gcttgaggaa
      301 taccgttcac tttttggtct tgccagtttg cgttttcagg gcgaccaaca tttgctggat
      361 attgccttct ggtgcaacga aaaaggggtc agcgcccgtc agcagcttag cgttcagcaa
      421 caaaatggta tctggacatt agttcaaagc gaagaggcgg agatccaacc acgcagcgac
      481 gaaaaacgca ttctgagtaa tgttgctgta ctggaaggtg cgccgccgct atcggaacac
      541 tggcaactgt tcaacaataa cgaagtcctg ttcaatgaag cccgtaccgc tcaggcggcg
      601 acggtggtct tttctttaca gcaaaatgcg caaatcgagc cactggcccg cagcattcat
      661 accctgcgtc gccagcgcgg tagtgcgatg aaaatcctcg tgcgggaaaa taccgctagc
      721 ctgcgcgcca ccgatgaacg tttgttattg gcctgcggtg caaatatggt tattccgtgg
      781 aatgcgccac tctcccgttg tctgacgatg atcgaaagcg tgcaagggca gaagtttagt
      841 cgctatgtgc cggaagatat cactaccttg ctgtcaatga cccagccgct caaactgcgt
      901 ggtttccaga agtgggatgt gttctgtaat gccgtcaaca acatgatgaa taaccctcta
      961 ttacctgccc acggtaaagg cgttctggtt gccctacgtc cggtaccggg tatccgcgtt
     1021 gaacaagccc tgacgctgtg tcgccctaac cgtaccggcg atatcatgac cattggcggt
     1081 aatcggctgg tgctgtttct ctcattctgt cggattaacg atctggatac cgcgttgaat
     1141 catattttcc cattgcctac tggcgacatt ttctcaaacc gtatggtctg gtttgaagat
     1201 gatcaaatca gtgccgagct ggtgcagatg cgcctgcttg ccccagaaca atggggcatg
     1261 ccgctgcctt taacgcaaag ttctaaaccg gtcatcaatg ccgagcacga tggtcgccac
     1321 tggcgacgaa taccagaacc catgcgactg ttagatgatg ctgtggagcg ctcatcatga
     1381 tgaccatcag cgatatcatt gaaattattg tcgtttgcgc actgatattt ttcccgctgg
     1441 gctatctggc gcggcactct ttgcgacgca ttcgcgacac cttacgtttg ttctttgcta
     1501 aacctcgtta tgttaaaccg gctgggacgt tacgccgcac ggaaaaagcc agggcaacca
     1561 aaaaatgact caatttacgc aaaataccgc catgccttct tccctctggc aatactggcg
     1621 cggcctttcc ggctggaact tctattttct ggttaagttc ggcctgttgt gggcgggata
     1681 tcttaacttc catccgctcc tcaatttggt gtttgccgcg tttctgctga tgcccattcc
     1741 gcgctacagc ctgcatcgct tgcgccactg gattgccctg ccgatcggct ttgctttgtt
     1801 ctggcatgac acctggttgc ctggcccgga aagcataatg agccagggtt cgcaggtggc
     1861 ggggttcagt accgattatt taatcgacct tgtcacacgc tttattaact ggcagatgat
     1921 tggggccatt tttgttttat tagtggcctg gttattcctg tcacaatgga ttcgcattac
     1981 cgtttttgtg gttgccatac tgctatggct gaacgtactt accctggcgg gaccaagttt
     2041 ctccttgtgg ccagccggac aaccgacgac cactgtaaca acgacgggtg gtaacgcagc
     2101 ggcaaccgtt gcggcgacgg gtggcgcacc ggtagtgggt gatatgcccg cacaaactgc
     2161 accgccaaca acggcgaacc ttaacgcctg gctgaataat ttctataacg cggaggcgaa
     2221 acgtaaatcg accttcccgt cttcgctgcc cgctgatgct cagccatttg aactactggt
     2281 gattaacatc tgttcgcttt cctggtcgga tatagaagcc gctgggttga tgtcgcatcc
     2341 actgtggtcg catttcgata ttgagttcaa gaactttaac tccgccacct cctacagtgg
     2401 cccggcggcg atccgtttac tgcgcgccag ctgcgggcag acttcgcaca ctaatctgta
     2461 tcaaccggca aataacgact gctatctgtt tgataacctg tcgaaactgg gctttaccca
     2521 gcacctgatg atgggacata acggccagtt cggcggtttt ttgaaagaag ttcgcgaaaa
     2581 tggcggcatg cagactgaat tgatggatca aacaaatctg ccggttattt tgctgggctt
     2641 tgatggttcg ccggtttatg acgataccgc cgtgcttaac cgctggctgg acgttaccga
     2701 aaaagataaa aacagccgta gtgccacgtt ctacaacacg cttccactgc atgacggcaa
     2761 ccattatccg ggcgtcagca aaacagcgga ttacaaagcg cgggcgcaga aattctttga
     2821 tgaactggac gccttcttta ctgaactgga gaaatcgggt cgtaaagtga tggtggtcgt
     2881 ggtgccggaa cacggcggcg cgctgaaggg cgacagaatg caggtatctg gcctacgtga
     2941 tatccctagc ccgtctatca ccgacgtccc cgttggggtg aaattcttcg gcatgaaggc
     3001 accacatcag ggggcaccga ttgtcatcga ccaaccgagc agcttcctgg ctatctccga
     3061 tctggtggtt cgcgttcttg atggcaagat tttcaccgaa gacaatgttg actggaaaaa
     3121 actcaccagt gggttgccac aaacagcacc ggtctcagag aactcaaatg cagtagttat
     3181 tcaataccag gataaaccgt acgttcgcct gaacggcggc gactgggtgc cttacccgca
     3241 gtaagcacga aaaaggccgc agagtttccc ccctgcggtc tggtccgggc gcaaattgcc
     3301 attacggcag cctgacgccc gcatgacacg ttacttccgc ttgttcagcc agttcacgat
     3361 catactggca agaatgccag caatgaccgg agccgctaaa tcatgccaga aggccatgcc
     3421 cagctctgcg agcgtcatat agccgcctgt gttgtaatga caacgtttcg cggctattct
     3481 tgagtggtct aggttcaaga ttagcccccg tggtgttgtc aggtgcatac ctgcaacgtg
     3541 cgggggtttt ctctctccag caaccaatgc caccagggat taagcccccg caacattgcg
     3601 cctcaccgga acccttccgg cttgccgcta attttacgat tgctttttac tgtcagcact
     3661 cggcaatctt cctcattgcg tgatgccttc cagcttaatt tcttaatttg caatagttgc
     3721 agttcatatc cgggctgact ccagactggt agtgaaatgc gcaattttgt agaattgcgc
     3781 ctcctcgcga cctccccagt gaatctgttg cctcattttt tacatactgc gtattcgact
     3841 tctccacctg ttgcgcaaga gaaactgggt ttattcattt ttgcgaggcc gacttctttc
     3901 tggacaggac tttatgcagc acaacacact accgaaacac gatcagaaat tgccgtttac
     3961 acgctacgac ttcggctggg ttttattatg cataggcatg gcgattggtg ccggaaccgt
     4021 gctgatgcca gtacaaattg gcttgaaggg catttgggta tttattaccg cagcgatcat
     4081 tgcttatcct gccacctggg tagtgcagga catttattta aaaacccttt ctgaaagcga
     4141 ttcctgtaat gactacaccg atattatcag tcattacctg gggaagaact ggggaatttt
     4201 cctcggggtt atctactttt tgatgattat ccacgggatt tttatctact ctctctccgt
     4261 ggttttcgac agcgcctcgt acctgaaaac cttcggttta accgatgccg atctttcaca
     4321 atctctactt tataaagtcg ctattttcgc cgtactggta gcgattgcgt ctggtggtga
     4381 acgattactg tttaagattt ctgggccaat ggtggtggtc aaagtaggga ttattgtcgt
     4441 gttcggtttt acgatgatcc cgcactggaa tttcgccaat ataaccgcct tcccgcaagc
     4501 ctccgtcttt tttcgcgatg tcctgcttac cattccattt tgcttctttt ctgcagtatt
     4561 tattcaggta cttaacccaa tgaatattgc ctatcgtaaa cgggaagcgg ataaagtact
     4621 ggcaacccgg ctcgcgctgc gtacccaccg aattagttat atcacgctca tcgcggtgat
     4681 actgtttttt gccttttcgt ttaccttctc aattagccac gaagaagccg tttctgcctt
     4741 tgaacaaaat atctcggcac tggcactggc cgcgcaggtg atccctgggc atatcattca
     4801 tatcacctct acggtgctga atatctttgc cgtactgacc gcattctttg gcatttatct
     4861 cggtttccac gaggccatta aaggcattat tctcaatctg ttaagccgaa ttattgatac
     4921 caagaaaatt aactcacgcg tgctgactct ggcgatctgc gcttttatcg tcattacgtt
     4981 gacgatttgg gtttcgtttc gtgtatcggt gctggtgttc tttcagttgg gaagcccgtt
     5041 atatggcatt gtgtcgtgcc tcattccgtt tttcctgatc tataaagtcg cacaactgga
     5101 aaaacttcgc ggatttaaaa cctggctgat tctgctttac ggcattttgc tatgcttgtc
     5161 gccactgttg aagctgattg agtaaaccgg agcgcatggc cccggttttg tgagttaacg
     5221 ctgcggattt tcatcctgat caacagcaaa acaagctacc agttgaccgc cgtagtcttt
     5281 tagctgcggc tgcaactggg tgcaggggcc gaagcgccga cgacagcggg cgttaaacgc
     5341 gcagcccggc ggtggattca gcgggcttgg cagttcaccg gtgagtttga tgcgctcgcg
     5401 gcgatcgtcc ggattcaggc gcggcgtcgc ggaaagtagc gcctgagtgt acggatggcg
     5461 cgggttattg aagatttggt ctttcgttcc cttctccacg cagcggccca ggtacatcac
     5521 catcacttca tcagcaatgt gctccaccac cgacaggtcg tgggagatga agacataaga
     5581 aagccccagt tcctgctgca aatccatcat caggttgagc acctgtgcac gcaccgagac
     5641 gtccagcgcg gagaccggtt catcggcaat caccacgtcc gggtcgagca tcagacctcg
     5701 ggcaatggcg atacgctgac gctgaccgcc ggagaacata tgcggatagc ggtcatagtg
     5761 ctcggttttc aggccgactt tcgccatcat cgacagggct ttttcccgac gctgttcttt
     5821 gcttaagctg gtgttgatca gcagcggctc ttcaagaatt tgcccgattt ttttacgcgg
     5881 attcagcgaa ccgtaagggt tctggaagac aatctggatt ttctgccgac gcagcttctg
     5941 cgcctgcgga tcgtgcttaa gcagatcctg cccctggtaa tacagctcgc cgccggtggg
     6001 cgtttcaatc atcgtcagca accgaccgag ggtcgattta ccgcagccag attcacctac
     6061 taccgccagc gttttgccac gttcaaggtt aaacgaaacg ccgtccagcg ccttcaccag
     6121 acgttccgga gcgaacatac ctttcttcac cggataatgt tttttcaggt cgatagcctg
     6181 caacagcggt tgttgcgagg tggcctcttg cgtactcata gtgtcggcct cccggcatca
     6241 tcaagtgggt aatggcattt ggactgacgc ccgtcagcga gcatattcag cgccggttct
     6301 tcagcgcgac atctgtcagt ggcatagggg cagcgcgggt taagcaagca gccgttcggg
     6361 cggtcgtact tgccgggaac aacgccaggc aacgatgcca gacgttcttt gtcctgagca
     6421 aattccggca gcgcacgcag caatgcctga gtatacgggt gacgcggcgc atggaagatg
     6481 gcgtgcgcat cgcccgtttc caccacctga ccggcataca tcacgatgat tttatgtgcc
     6541 gcttccgcca ccagcgccag gtcatgggta attaacacca gcgccatgtt ctctttctgc
     6601 tgtagctcca gcagtagttc aatgatttgc gcctgaatgg tcacgtccag cgcagtggtt
     6661 ggttcatcgg caatcagcag ttttggccga caggcaatgg ccatcgcaat catcacgcgc
     6721 tggctcatgc cgccggaaag ctgatgcggg taaacatcca gacgcgaagc cggatcggga
     6781 ataccgacct gattcaacaa gtcaatcgcc cgctgacgac gagtgctctt attaccgccc
     6841 tgatgcacct taatcgcttc cataatctgg aaacccacgg tgtagcacgg gttaaggctg
     6901 gtcatcgggt cctggaagat catcgccact tcggcaccca ccaggttgcg gcgctctttt
     6961 tccgagatac gctgcaaatc ctgaccgtta aactccagct tttccgccat cacgcggccc
     7021 ggataatcaa tcagccccat aatcgccagc gaactgaccg acttaccgga gccagactca
     7081 cccacaatcc cgactacttc gccctgtttt acgctgtagc taatgcggtc tacggcgcgg
     7141 aacggtgcgc tttcgtcgcc gaaatgcacc gataatttat ctacatttaa taacgccatc
     7201 tcgaacctct tactgcttca gtttgggatc gagcgcgtca cgcagaccgt cacccatcag
     7261 gttaaatgcc agcaccgtca gcaggatcgc cagacccggg aaggtcacga cccaccaggc
     7321 gctttgcgcg aactgcaaca cgtcggagag catggtgccc cactccggcg ttggcggctg
     7381 cgcccccatg ccgaggaaac caagagcagc catatcgaga atggcgttag agaaaccgag
     7441 cgacgcctga acaatcagcg gtgcaaggca gttcgggaag atgttaataa acatctggcg
     7501 catcgccccg gctcctgcca cgcgagaagc ggtgacgtaa tcgcgattca cctccaccag
     7561 cacggcggcg cgggttaagc gcacatagtg cggcaaggca acaaaggtca gcgccagcgc
     7621 ggcgttacca atcgacgggc cgaaaatcgc caccagcacc agtgccaaca gcagacttgg
     7681 cagcgccagc atgatatcga ccacgcgcat aatgatgtta tcgaccaggc cgccgaagta
     7741 accggcgatc aggccgagaa taacgcccat aatcagcgat aacaccacca ccagacagcc
     7801 aaccagcagc gacaagcgcg cgccgtacat cagccgcgac agcacatcac ggcctacatc
     7861 atcggtaccc agcaagtgcg ccatgctgcc gccttcctgc caggctggcg gggcgagcag
     7921 tgcatcgcgg aactgttccg ccgggttata gggagcaatc cagttggcaa agatcgcgat
     7981 aaacagcacg atgacgacgt aaaccagccc gacgaccgcg cctttgttgc gtttaaaata
     8041 gtgccagaac tcctgtaaag gggtcatcgg taccggcgcg ctaatcactt tattttcagt
     8101 aacctgtgac atgatgaccc cttacttctt atgacgaata cgcgggttca ccacgccata
     8161 cagcagatcg accagcaggt tgacgaggat aatcatcgtc gccaccagca atacgccgcc
     8221 ctgcaccacc ggataatcgc gccgttgcag tgcgtcaatc aaccagcgcc ccagaccggg
     8281 ccacgagaag atggtttcgg tcagaatcgc ccccgccagc aatgttccca cctggaggcc
     8341 gataacggtc actaccggca gcatcgcgtt acgcagcgca tggacgataa tcacccgcat
     8401 gcgggtcaac cctttggcgc gcgcggtgcg gatgtaatct tcacccagca cttccagcat
     8461 cgaggagcgt gtcatacgca caatgaccgc cagcggaata gtacccagca caatggcggg
     8521 caagatcata tgggcgacgg catcgataaa gttaccgtct tcaccccaga tggcggtgtc
     8581 gatcagcata aaaccggtta acggattgga atcatcgagg aacaccatat cgctcacgcg
     8641 accggagacg ggtgtcaggt tccagtgcac cgaaaccagc atgatcagca tcatgcccca
     8701 ccagaagata ggcattgaat aacctgtcag cgccaggcca accgctgtgt gatcgaaaat
     8761 ggaaccgcgt ttaaccgcgg ccagcacacc aaccggaatg ccgaccgcgg tcgcaaaaat
     8821 catcgcgcag acgccaagtt ccagcgtggc ctgaaagcgc ggcacgaact cttcccatac
     8881 cggaattcgg cttttcattg aaatgcctag atcgccatgc ataacgcccc aaatgtaatg
     8941 gagatactgc tgccacatcg gtttatctaa gccgagttca gccagcaact gcgcgtgacg
     9001 ctctggggag atcccacgtt cgcccgccat gatcatcaca ggatcgcccg ggatcatgtg
     9061 gacaaaggca aatgtgagaa gggtaatacc gataaacgtg gggatgacga gtcccaaacg
     9121 tcggagaata aactgcaaca taacccggat tctctgtagt gacccgcagc ctggaactgc
     9181 gtgtctgtat tgctcacaaa tcaattcccg cccaggtcgg gcagggaatg aagtattgct
     9241 gccggatggc gctgcacgca gaggcacagc accaccgggt atggctttta attattcgat
     9301 agagacgttt tcgaagtgat gtttgcctaa tggatcaacc acatagcctt taacttcttt
     9361 gcgtaccggt tcaaacacgg tggagtgagc gatgatcagt gccggagcct gatcgtgcat
     9421 caccacctgc gcttgcttgt acagttcaac gcgtttgtta tggtcgtcgg tagcacgcgc
     9481 cggttgaatc agatcttcaa acggtttgta gcaccatttt gagtagttgg agccttgttc
     9541 agaggcggcg cagctgaaca gagtggcgaa gaagttatcc ggatccccgt tatcgccagt
     9601 ccagcccatc attaccgtct ggtgctcgcc atctttcgca cgcttgaggt actcacccca
     9661 ttcgtaggtg acaattttgg cctgcacgcc gactttcgcc cagtctgcct gaatcatctc
     9721 cgccatgcgg cgagcgttcg ggttatacgg acgttgtacc ggcatcgccc acaggtcgat
     9781 ggagaaacct ttttccagac ccgcttcttt cagcaaggct ttcgcttttt caggatcgta
     9841 ggtgtagtcc tgaacgtcgt cgttatagcc ccacatggtt ggcgggatca ggtttttcgc
     9901 tgatacaccc gcgccctgat aaaccgcttt gatgatcgcg tctttgttca ccgcgtaggt
     9961 cagagcctgg cgaactttca cgtcatcgag tggttttttc tgcacgttat acgagagata
    10021 accgacgttc agccccggca tttccatcag gttgatagat ttatcctgct tcatgcgggc
    10081 gatatctgcc gggttcgggt acggcatcac ctggcattca tttttctgca atttagcgta
    10141 acgcacggaa gcgtcagggg taatagagaa aaccagcgta tcgatctgcg gtttggtgcc
    10201 ccagtagcca tcaaacgctt tgtagcggat acgggaatct ttttgatact gctgtaactg
    10261 gaacggaccg gtgccgattg ggttgaggtc cagtttttcc ggtgtaccgg ctttcatcat
    10321 cgcatcagca tattcttttg acagaataga ggcgaagtcc attgccaggt cagcgaggaa
    10381 cggcgcttcc gggcgagtca gcacaaactg aacggtgttg tcgtccacct ttttcacttc
    10441 gctgatcagc tccggcaagc ccatgccttc gaagtattcg tagctgccgc cagaaacttt
    10501 atggtacggg ttttgcgcgt ttttctgacg atcgaacgag aacaccacgt catcggcatt
    10561 catttcgcgc gtcggtttga attctttatt gtcgtgccac ttcacacctt tacgcagatg
    10621 gaaggtatag gttttaccgt cttcgctgac ttcccacttt tcagcgaggc ccggaatcac
    10681 ttcggtggtg ccgattttaa attcaactag gcggttataa agcggtacgg aagaggcgtc
    10741 ataggtggta ccggaggtaa acagctgcgg gttaaaccct tccggagatc cttctgagca
    10801 ataaaccaga gttttagcct gaacacttgc tgcgacggtc atagccacca ggctgagacc
    10861 aagcttcagc atccctgact ttttcaagga aatacgcatt attctgctcc aattgtgatg
    10921 tttgttgttt taaccctttg cagtgggttg tcgctgcctg accttttttg ttttttgccc
    10981 ggtcgggtca acgttatgag gtggggatgc cgtactaatt aacatcagtg taacagtgcg
    11041 gatcctccat aaaatgcccc tcgtgaccta caatctgtca acagaatgtg aaaacgtcaa
    11101 tacagccaac cgggatttac accaacggtg agaatccaca aacaaagatt aaaaaaactt
    11161 caaacagcta tttgcagcag agaaatttgt gctactccaa catgaccaga acaatcagct
    11221 taatatttag caacatttgg tgattaagtt ttatgcattt agtgaaaaaa atctgaggaa
    11281 aaggtggata tctgagcgat taataccatg aacgctaaaa cgcacagcgg aaaatgccag
    11341 cgccagccat aagtaacgca aaaaaagatt taagcaaata taaaaaaaga caatggtttc
    11401 tgtcacaaat ccgttaatgg atagtgagat atggggcgca aatttggcag ggaaatgtgt
    11461 ggcgtgtagg tcgaataagg cgttcacgcc gcatccgaca ataagtgcgg tgccgttgcc
    11521 tgatgcgacg ctgacgcgtc ttatcaggcc tacgtcgagt cgaacctccc tgcccccgga
    11581 ggttctcttc ctcatgggct gcagatgcaa aaaagcctgc tcgttgagca ggcttttcga
    11641 atttggtcgg tgatagagga ttcgaacctc cgaccccttc gtcccgaacg aagtgcgcta
    11701 ccaggctgcg ccaatcaccg aatgcggggc gcatcttact gcgcagatac gccctcgtca
    11761 atcccttaat agcaaaatgc cttttgatcg gcgagaaagt cagcaggcct cttagttagc
    11821 cgctgcctct tttgtctgcg ggatgtgaca ccagttgttg ttttcgttaa ttccaccatc
    11881 cggtgaagta tagccaagac aacccatgat ggtgtcgtac agctctacgt gacggcgtgg
    11941 cactttcatg tcggctgctt ttttcagctg cgcaaacgcc tgcgcattga ccggattttc
    12001 cagatattta tctgacatcc agaccatcat cggtacgcgg aactgctccg gcggtgccag
    12061 ttcacgcggc gtgccgtgca ggtgctcgcg ttcattaatt gactcgccgt ggtcagctgc
    12121 gtagaacaca atcgctttct tatcgcgaac ctgatcaatc acactggaga taaagtgatc
    12181 cacataggtc accgagttgt cataggagtt gatcatctgc gctttggtac atccgctatc
    12241 aacaccaata cattccggct tccactgcgc aaagctgcgc gggtaacgct gggtgtagtt
    12301 aaagtgcgaa cctttggtat gcagaatgat tagatgctta ccatccgggt tgcgccccag
    12361 cgattgctgc atttcgtcta ccagcaacat atcatctacc ggcttgccac ggttacgtgg
    12421 ctccgcacca atctgttcac ggtaagcaat attgtccgcc atcgtattgc tgtagaacca
    12481 catttcgctc tgcatagcgt agaggtcaga actgaatcct aactgcttca gtaccgcgaa
    12541 aatgttctgt tcttttaatg tgcgctgcgg attatcttcc gcgcccccct gacgtacaaa
    12601 catgcaacgc agagagagtt tggttgcggt atcacaggag taaccacgga acgccgccag
    12661 atttttctcc tgggccagct tcggcgtggt attacgctca tagccgaaaa tgcccatatg
    12721 gtcccaacgc gtggtttcac cgatgataaa cacgacataa gtatcattaa cattttgcgg
    12781 tgcctgataa gtgaatttct tcgccgggtt aagcaatgaa ttattatcgg aagattcatc
    12841 gacccgcgcc caggcgtaca gccccaacgc agaaagccag tttgatggca gataagagtt
    12901 cgctacgaca ccgccataac tcggcaaatc aacgccggtc gccctctcca ctttcttctg
    12961 ctggatatcc agcaaacgaa tcggtgccca aaccataatt cccgccagta cgacgaccgc
    13021 caggctgcga atacgctgcc ctggggttcg cagttgtcgg agcaaggtgt agcgacagcg
    13081 gttattccag ataaggataa gaggcaatgc gctaacggcg attaaccaga gaataaagtt
    13141 cagaccaaca acttcttttg acaggtcgat atcggtggtc atgacggaag cgatgatgcc
    13201 ataaccaatg accacattaa ggaaggtcat gtaatagctg gcacctgcgg aaaagagcac
    13261 aaccagcgat gccagaatac gccagctgcg gcggccaaac agcgaaagaa gacgtagtaa
    13321 aaagaaggtc accagtacgg tggcggccag ttcaacaaca gcagaaatgc ctttccagac
    13381 ggtaaaatcg tgcgcatagc tgccgaagcg gcggtaaaaa accgcgccat tcataaaaag
    13441 gccaatatag attgcaagca aaaagctcag cttctgctgt gtaatcgatt tgatgtatct
    13501 catgcaaaca aaccctggac aaacaggtga taacaccctg cttagcggct tttcaggccc
    13561 agcaggggaa aagtgcggag gtagtcagat aagtaaaatc tacacgcgcg aataagtaga
    13621 ccacagcaaa gggaaaaagt gtggggaaag agtgtgcatg aagcacaaat ttacaaaagt
    13681 gcaagtccgg aatagaccat tttaacggat taacgactat tcagagatag gttgggccag
    13741 gatgctggcc cagtaactta ctcataggtg atggaaaaat tgacaagggc attcgctgtg
    13801 ccaccagtgg cctgagtgtt gacgaccaca taactcgcct ggtagtgaag cgacgtggtt
    13861 tcgccatcaa tcgcccattc aatcggcgtt ccgctggagc ctttgggaac ttgctgcgtt
    13921 cctttatcgt cctctatctt caacgcaaca cccgttgcgc caccagcctc aagggcaaac
    13981 atggaatcat catcgctgtc cgactgactg tccatctcaa cggtcgcttt ggatatccca
    14041 tcgcactctt tgagttcaat ggtgaaatct ttcttgctca acaccgtacc gactgccgca
    14101 ggtttttgca gcgtcattgt tcccatatcg acttcgagat cgcttttttc catctcgcaa
    14161 gctttagtgg tgatagtgat ggagaaatga atatcccctg ccgggccaag atccgtcgcc
    14221 gcattcaccc cgcaggcaac cagactcaaa ctggcgatgc cgctatgcag caatcgtttg
    14281 aacttcatgg catcactcca tttcaagata tatggtcgtt acgccttcaa acgtcccgcc
    14341 tttaggcgct ttacccgtcg tgctaatcgg taaagccagc agtcgaatcg cggcattgcg
    14401 gtgagtttta gaatccaggc tgctcaaatt taataacgtg gtgctgcttg aatcattcgg
    14461 taccaaaatt ttgccgctgg aatctttcac cagtacgcca atatcggggt tgcccatatc
    14521 caccgctcgg ggatcgtcag cattggttgc cccctcaagg cgcaacgcaa ccttgatacc
    14581 atcagagata ttgttgcaat cgaagctgag gttaatttct tgctctgtcg caccttcagg
    14641 catttgccct tgcctgtttt taaacgccga ggaggggaat tcaccaaatg gcacatccag
    14701 caccgtgccg ggtttaattt cgcaatcctg ggttacggtg acacgcccct gaatagatac
    14761 ggaagatatg ggtattgttc cgtaatcttt atttgttttc gaggcataca gttcggcaat
    14821 taacgtcaga gggatgacgg aatcgccgat aaagggttta gtaatagcaa aggaaagtag
    14881 acctgcgccg cccatatagg gcgtagtaat gactttgttg catccggttg tttgcagcgc
    14941 ctgctggttt ttgaaaggaa caaacccata cacactggta ctgatgcgaa tgttcgtcgc
    15001 aacctccagt tcctccgtca aagtataata tttcatactg tcaaccgtac ggccagaagg
    15061 gggaagcata ctcaccccct gcactagagt atctttttca ttcttataag aatcaggaca
    15121 ttcacaaatt agcgatacgt tttgtccatt agaccagtta aggacatccg gtaaaaccgt
    15181 tcctgcgacg ttttttgccg catcaggtat accaacatcc acatcgacat tatagtgata
    15241 agtcgtgcca tccgcggggg tacatggccc ccacgcatct ccggcaaatg ccttgccaga
    15301 aaatccgaat acgcatccta ataaggataa tgctattgcc gctttcattt cgctctcctt
    15361 gcttactggc aattggccga aacaggaata attccgccct gttttaattc cagggataat
    15421 ttataaggcg ccacgcattg ctgcgacgcg tcattacccc atttaatcaa tacccgtcct
    15481 tcctctggca ttccgctaat atagagctgt ccttcttcac caacgatact gctcacctcg
    15541 ttttgtttat cggtgattag cgttgccgtt gctccaaagg gcaccgggct gcccgcggaa
    15601 cgaagcagcg tcattaatac gcggtaacca acgcgggtat caaaacgcgc tctgacaatc
    15661 gctccacgcg ttggcaccac tttctggaac gcattttcaa cgtcaacatc gtcacccaac
    15721 gaatcgctac gcagtgagat ttcgttttca cgatacggca tggcataagg cacgatcgcg
    15781 ttacccatcc agtcaacctg aacgccgccg ttattcacca cgcgggcatt gcgcgcaccg
    15841 ggtacggaga tgagcgtcat ggtttcgcct agcggttggg aaagcgtcac gccttcgcta
    15901 tgaactatca cgccgccgcg aacgctgtag ttgagctgcc ggtaatcttt cccgtagcta
    15961 tagcctacgt tgacattacc ggacccaccc tgatagccaa cgttagcgtt accgctgtta
    16021 cccacaccgt tgctggcgta gccttcctgg acactgtagc tcaggttgcg atcctcaagc
    16081 agcgttccgc tgaccccaac ctgttgattg gtacgaccat cctggtcggt cgtgacgcga
    16141 tagttactcc aggcccggcc tagtggaacg gaaatattga aagaccacaa gcgatcgctt
    16201 tcatcccatt cagggctttt attccagcta tatgcaatac tgtaactgac cttgccaata
    16261 cgtccgttgt aacccgccga taccgtgttc tgtttacctg cgtcgttcca gtaatcctgc
    16321 tgcgttaaat ttaaataaac agagccatag gcccctaatt gctgcgtcag gttaccctgt
    16381 atttcgctgc gcttgtgata acggttatag tcgctgtcag cgtcactgcg cacatcggtg
    16441 gcttcctgga aggtatagaa accgctggta gagtaccgat agcccgcaag gcggaaatcg
    16501 gtgccgcttt cgaagctctt ggaatataag aaacgataag attgtccctg gctatcgcga
    16561 tcgttattca gttcgctttt agcctgcgtc acatcaatgg aaatcgcccc gatataacca
    16621 aagtttttcc ctatccctaa tgtaaatgca ttgtaattat tagagattaa tacgccgccg
    16681 taggccgtca tcccccacgg taaaccgtag atcagatcaa gctgcccgaa acgcggcgag
    16741 gcgctgtcat aattcccggc ctgatattca cctgcggcaa agctatattt gagatggcct
    16801 tcacgctgga aaattggcac cgatgagaat ggctaaataa agcgttgttc actgccatcc
    16861 gcttctttaa taatgaccgt gagatcaccg ctgtttgcag tggggtaaag atcgtttatt
    16921 tcaaaggcac caggctgcac ataggtgcga taaataacgt agccgttttg ttcaatactg
    16981 acttcggcgt tgcttttggc gatccctcga acgatgggtg caaagcctcg ctggctgtcg
    17041 ggcagcatct cttcatcaga ggctaacatc gcgccacgca tctgaacgct gtcaaaaatg
    17101 tcccccgcgg tcgccgtatc ccccattgtt aattgcgctt tgaaagggat gatggcccta
    17161 ctcaaagacg taccgatatt atcccactgc gctttgccac cggaatggct ccaggtactg
    17221 taattccgta accgccaagc gccaaggttc agcccgctgc gtaagttgag gtagtagctg
    17281 tcgctcttta gcgtatcttt accatcatca tgatgaacag tgccattatc atcgacataa
    17341 ctgctggaag accccgtcga gtcatattca ctgttgctcc cgctaaaact gtaatccagc
    17401 agcaacgcag ggataccttc gtcccataaa gattccggca ccgtgccgcg cgccacctgg
    17461 tgcatcgccg cctgagggaa acttaatacc agcttttgtt cactgaagtc gaaatggctt
    17521 gaggccaggg gaatgatttc gtcaaacggt acgcaggctt cggcagacac cagattgagg
    17581 gccggaaagc tttcaacgcg cacgcccatc tctgccagtt gcgtccggct aaggcaaggc
    17641 acaagggatt cccccatcgc tttgcgctgt gcttctgtgg cctctttaaa ttccagtgtg
    17701 cgggtttcca ttctctgttc attgacgtaa acagagacac gatagatgcc gggtaactgc
    17761 tgttcctggc ctttttcgaa catcgttaaa tcgacatgct ggcccacacc ctgaacgtcc
    17821 tccaggaatt tggggttaaa ctttccggca tgtgcagaaa acgtgctggc aacagcaacc
    17881 gccaccacag agatagaaaa ggaagaaaaa gtacgtgaaa cggtcttccg tgacattgag
    17941 ctatttctcc tgaaaagata ttaatcaatc aacattttta gtgatcattt ccgctgacga
    18001 tccaaagtca ttgatggtgt tatatttaat tttagaaacg ctacctaccc cggcgggtaa
    18061 tgtgatattt aatgcccctt tagcgggtac catgcctgct ttttcaattt c
//
LOCUS       DBFIFM010000085        18058 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.085, whole genome shotgun sequence.
ACCESSION   DBFIFM010000085 DBFIFM010000000
VERSION     DBFIFM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.085"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(145..1737)
                     /gene="lsrK"
                     /locus_tag="N7C74_002488"
     CDS             complement(145..1737)
                     /gene="lsrK"
                     /locus_tag="N7C74_002488"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310145.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HGU2432336.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTIRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKVGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSTIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1816..2769)
                     /gene="lsrR"
                     /locus_tag="N7C74_002489"
     CDS             complement(1816..2769)
                     /gene="lsrR"
                     /locus_tag="N7C74_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HGU2432337.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3018..4553
                     /gene="lsrA"
                     /locus_tag="N7C74_002490"
     CDS             3018..4553
                     /gene="lsrA"
                     /locus_tag="N7C74_002490"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HGU2432338.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITSADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTSVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNDKEINRLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLELYRRALNI
                     KYNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     AAC"
     gene            4547..5575
                     /gene="lsrC"
                     /locus_tag="N7C74_002491"
     CDS             4547..5575
                     /gene="lsrC"
                     /locus_tag="N7C74_002491"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HGU2432339.1"
                     /translation="MLKFIQNNREITALLAVLLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALELNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5575..6567
                     /gene="lsrD"
                     /locus_tag="N7C74_002492"
     CDS             5575..6567
                     /gene="lsrD"
                     /locus_tag="N7C74_002492"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HGU2432340.1"
                     /translation="MRIRYGWELALAALLVIEIVSFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGAIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6579..7601
                     /gene="lsrB"
                     /locus_tag="N7C74_002493"
     CDS             6579..7601
                     /gene="lsrB"
                     /locus_tag="N7C74_002493"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HGU2432341.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7628..8503
                     /gene="lsrF"
                     /locus_tag="N7C74_002494"
     CDS             7628..8503
                     /gene="lsrF"
                     /locus_tag="N7C74_002494"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HGU2432342.1"
                     /translation="MADLDDIKDGKDFRTDQPQKNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGTQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8527..8817
                     /gene="lsrG"
                     /locus_tag="N7C74_002495"
     CDS             8527..8817
                     /gene="lsrG"
                     /locus_tag="N7C74_002495"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HGU2432343.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8874..9632
                     /gene="tam"
                     /locus_tag="N7C74_002496"
     CDS             8874..9632
                     /gene="tam"
                     /locus_tag="N7C74_002496"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HGU2432344.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENVDYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGRESLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLKEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9636..10550)
                     /locus_tag="N7C74_002497"
     CDS             complement(9636..10550)
                     /locus_tag="N7C74_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HGU2432345.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQVEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKVLPDRHYQLT"
     gene            complement(10747..12198)
                     /gene="uxaB"
                     /locus_tag="N7C74_002498"
     CDS             complement(10747..12198)
                     /gene="uxaB"
                     /locus_tag="N7C74_002498"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HGU2432346.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12425..13843)
                     /locus_tag="N7C74_002499"
     CDS             complement(12425..13843)
                     /locus_tag="N7C74_002499"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGU2432347.1"
                     /translation="MHVHPISTFRVFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(13982..14341)
                     /locus_tag="N7C74_002500"
     CDS             complement(13982..14341)
                     /locus_tag="N7C74_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HGU2432348.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPHGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14341..15267)
                     /gene="glsB"
                     /locus_tag="N7C74_002501"
     CDS             complement(14341..15267)
                     /gene="glsB"
                     /locus_tag="N7C74_002501"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HGU2432349.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15331..16719)
                     /gene="sad"
                     /locus_tag="N7C74_002502"
     CDS             complement(15331..16719)
                     /gene="sad"
                     /locus_tag="N7C74_002502"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HGU2432350.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVTQMIKDSRIAAITVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMDDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16820..17701
                     /locus_tag="N7C74_002503"
     CDS             16820..17701
                     /locus_tag="N7C74_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU2432351.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWCWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSTKQGYQ"
     gene            17779..>18058
                     /gene="yneK"
                     /locus_tag="N7C74_002504"
     CDS             17779..>18058
                     /gene="yneK"
                     /locus_tag="N7C74_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HGU2432352.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICNE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPP"
BASE COUNT         4531 a         4596 c         4496 g         4435 t
ORIGIN      
        1 atttcccctt gaatatagta ctggtgatga aacgatgaat ctgcaagctg gcatttaaca
       61 agccagctct aaaagaaggg aaataagaat aactatactc aaaaactaac agccacggtc
      121 atcatgatgt ggctgtcaat gaaactataa cccaggcgct ttccataacg acgtcgtcag
      181 tccatgatca accagcccca gctgatcctg ataaactgcc tgccacttat cgcgtgaatc
      241 ctgataaagt tcatgctttt ccgggtctgg tgtgtgcgtc cgttcccagc gaaccaggcg
      301 ttctccggtt tctgccattg atgaaaaaat tccggcaccg acgccagctg caatggcaca
      361 tcctaatgca gtggcttctt tgaccaccgg aatattgacg ggtaatcccg agacatcagc
      421 gagaatttga ctccataatt tcccttttga acctccgcct gcaaagacta acgatgaagg
      481 atgaatagtc gaaaaatcag caatttgctg caagttacac gctgatacaa tcgccgcatt
      541 ttcttccagc gcacggaaca atgtcgcttt gttacattta tccggatcaa tggacaaatt
      601 aataaaggaa ggcgcagcgt gataccaggt tttaaagcgc attctgtcgg agaagatcgg
      661 cattacgccc cacgacccag gcggcacccg actggccatc tcttccagca gcgtataggt
      721 gtcgatgcct aaacgttcgg caatcagttt ttcttcggca cagaaagcat cgcggaacca
      781 gcgcatggtg agtccggtaa aaaagcttat agattcagct tgtaccatgc caggaataac
      841 atgaggatta acgcgcacgt tcatttctgg gtctgtcacc ggcgcggcta aatttacaac
      901 ttgctgccag aatgtgccgc caagaaccgc ggtttgtgcc ggacgcacaa cgcctaaccc
      961 caggcaacca agctgcacgt cgcctcctcc aacgaccacc ggagtgccca ccttcagacc
     1021 gcagagttcc gccgcttgtg aacttaccac gcccagcaat gtgccggttt ctttgacagg
     1081 agaaagaata tcggcacgca ggccagccat atccagcaat gcaggtttcc agtcacggat
     1141 ggttagatca agaagtcccg tggtgccagc gttagaggga tccaccgcaa gttcgccgct
     1201 gagcatatag gccagccagt cgctgatcat ggtgatggtt gatgcctgac ggtaaatatc
     1261 ggaacgatgg tgcgccagcc aaagtaatct ggggatggca cttaaagcca gtgtttgtcc
     1321 ggtcgcgcga taaacttcgt tttcaaaggt attgttgtgc agttctttaa gttcgctaac
     1381 ttcgcgcgcc gctctggcat ccacattggc gcaggcccag atcggtgttc cttcattgtt
     1441 atataaaaca atgccttcac gcatcgaaca tgccgaaacg gcagcgatat actccggggc
     1501 tatgccggcg ttgtgcagcg cctggcgcat acactcacac gccagttgcc agtttttgtt
     1561 gagatcaaat tccatagaac caggaacgtc cggtactgcc agatgccgcc actccgcctg
     1621 tcccactgct atttgattgc cttccaggtc gaatatcaca gcccgaatac ttccggtgcc
     1681 tgcatccagc gccatcaggt agtactttga ttctgaaggg gtaaagagtc gagccattag
     1741 attatcctcg gcttatgcgt tctgtctgtt cctctatacg ttctccatca ttcccggtaa
     1801 taaggtcatg caaatttaac tacgtaaaat cgccgctgct gtgtcctgat cggtaaccag
     1861 tgcgttgata taaccgcctt tcattgcagc ggcaattgct tcggctttat tttctccccc
     1921 tgccacgcca acccggacgg gtatggtctt cagcgcgctt aaaggtaagc caatcagctc
     1981 gttatgtatt ttaatgtccg tgacaacgtc accttttgca tcaaaaaagt agcctaaaat
     2041 gtcgccaacc gccccttttc ggccaatcat taactgttcg ccctggctga tataaccgga
     2101 gcgaatgatc gtcgcgtcgt cttgttgact aacggcccca atgccgacaa tcgccacatc
     2161 cgctgcttgc gcggctaaca gaacatcttt gacacaattt tcatttttta gcgtacgggc
     2221 aatgtcagcg gaggatgccc gcaacggagc cggaataata ttcacactgc acgccgcatt
     2281 aagctgtccg atacccgtca tataagaacc gacgccaccg gagagcgtga ccaggcgaat
     2341 ttgctgtgac gaaataaaac cacttaagcg ttgcagcgta ttcatggttg cctcgccaaa
     2401 accaatcgcc agcatctgtt gtggttgaag taaactcatc aacatatgcg ccgcgcctat
     2461 ccccagtcgc ccgccgacat cagcatccgc aagcccaggg atcacccgga catgttgcag
     2521 cgaaaactga cgacgtaatt gtgtttcata ttccagacag ccttcaaagc gagaattaat
     2581 ctgcacgcga atgatgccgg actgatgccc tttctccagt aatcgcgaca ctttcaaacg
     2641 ggtcaggccg agacgatcgc tgatctcact ctgggtcagc ccgtcgtggt agtaaaacca
     2701 cgcgatccgc gcgacctgtt cttcttcaca cattccctgt tctgaaatta ccgaatcgtt
     2761 gattgtcata attcattctt cactttgaac atatttaaat ctttaatgca attgttcagt
     2821 tcttgcgcat ttatatctgt gatggcaacc acagtttgac tctacgagca tgaacaaacg
     2881 caaccgtgaa aatcaaaata gtataaattg tgatctattc gtcggaaata tgtgcaatgt
     2941 ccacctaagg ttatgaacaa attaaaagca gaaatacatt tgttcaaaac tcacctgcaa
     3001 aactgaacgg gggaaatatg caaacgagtg atacccgcgc gttaccgcta ctttgcgccc
     3061 gctcggttta taaacagtat tcaggggtca atgtcctgaa aggcatcgat tttacgttgc
     3121 atcaggggga ggtccacgcc ctgctcggcg gcaatggtgc cggtaaatcg acgttaatga
     3181 agattattgc cggtattacc tctgctgata gcggtacgct ggagattggg ggcaacaact
     3241 acgccagatt aacgccagtt catgctcatc agctgggtat ttatctcgtt ccccaggaac
     3301 cgctgctttt cccaagcctg tcgataaaag aaaacatcct gtttgggctg gcaaaaaaac
     3361 agctctccat gcagaaaatg aagaacttgc tggcggcgct gggctgccag tttgatctgc
     3421 atagtctggc aggatcgctg gatgtcgccg atcgccaaat ggtggaaatc ctccgcgggc
     3481 tgatgcgcga ctcgcggatt ctgatcctcg atgaacctac cgcctcgctt acccctgcgg
     3541 aaaccgaacg cttgtttact cgcttgcaag agctgcttgc tactggcgtg ggtattgttt
     3601 ttatctcgca taagctgccg gaaattcgcc agattgccga tcggattagc gtgatgcgcg
     3661 acggaaccat cgccttaagc ggcaaaacca gcgaactgtc taccgacgac attattcagg
     3721 ccatcacccc agcggtacgg gaaaaatcgc tctctgccag ccaaaaatta tggctggagt
     3781 tacctggcaa ccgcccacaa catgccgcag gaacgtcggt gctgacactg gaaaatctga
     3841 ctggcgaagg tttcaggaat gtcagcctga cgctaaatgc cggagaaatt ctgggtctgg
     3901 ctgggctggt gggtgccgga cgcacagaac tggccgagac gctctatggt ctacgtactt
     3961 tgcgtggcgg acgcattatg ctgaatgata aagagatcaa tagattatcc actggagaac
     4021 gtttactgcg cggtctggtt tatctgccgg aagatcgcca gtcatccgga ctgaatctcg
     4081 atgcttcgct ggcctggaac gtctgcgccc ttactcataa ccttcgtgga ttctgggcga
     4141 aaaccgcgaa agataatgcc accctggaac tttatcgtcg ggcgctgaat attaaatata
     4201 accaaccgga acaagctgca cggacattat ccggtggtaa ccagcaaaaa atcctgattg
     4261 ccaaatgcct ggaagcctcg ccgcaagtat tgattgtcga tgagccgacg cgcggcgtgg
     4321 atgtctcggc gcgtaatgat atctaccaac tgttgcgcag catcgccgca cagaatgtgg
     4381 ctgtgctgct tatctcctct gatctggaag agatcgaact gatggcagat cgtgtgtatg
     4441 tgatgcatca gggcgaaatt acccactctg cactgaccgg gcgcgatatt aatgtcgaga
     4501 ctattatgcg cgttgccttc ggcgatagtc agcgtcagga ggcggcatgc tgaagtttat
     4561 tcagaacaac cgtgaaatca cggcactgct ggcggtgctg ctgctgtttg tattacccgg
     4621 ttttctcgac cgccagtatt taagtgtgca aacgctgacc atggtttata gcagcgcgca
     4681 aatcctgatc ctgctggcaa tgggcgcgac gctggtaatg cttacgcgca atattgatgt
     4741 ttcagtgggt tcgattaccg gaatgtgcgc ggtgctgttg gggatgttac tgaacgcagg
     4801 atattcacta cctgttgcct gtgtcgcgac tttactgctt ggtttgctcg cgggattttt
     4861 caacggtgtc ctggtcgcgt ggctaaagat ccctgccatt gttgccaccc ttggcacgtt
     4921 agggttgtac agaggcatca tgttgctgtg gactggcggt aaatggattg aagggttacc
     4981 cgccgaactg aaacagctct ccgccccgct gctgcttggc gtttcagcaa ttggttggtt
     5041 aacgataatt ctggtggcat ttatggcctg gctgctggcg aagacagcgt ttggacgcag
     5101 tttttatgcc acgggcgata atttacaggg cgctcgtcaa ctgggcgttc gtacggaagc
     5161 cattcgcatt gtggcatttt cgttgaacgg ctgcatggcg gcactggcgg gaattgtgtt
     5221 tgcttcgcag attggtttta tccccaacca gaccggtacc gggctggaga tgaaagcaat
     5281 tgcagcctgc gtgctgggcg gcattagttt gctcggcggt tccggtgcga tcatcggtgc
     5341 ggtactcggc gcatggttcc tgacgcagat cgatagcgta ctggtgctgt tgcgcattcc
     5401 ggcatggtgg aatgatttta tcgcgggtct ggttctgctg gcggtgctgg tgtttgatgg
     5461 acgcctgcgt tgtgcgctgg aacttaatct gcggcggcaa aaatatgccc gctttatgac
     5521 gccaccgcca tccgttaaac ccgcttcgtc aggtaaaaaa cgggaggctg cataatgcgt
     5581 attcgctacg gttgggaact ggctcttgcc gcactgcttg ttattgagat tgtctcattt
     5641 ggtgcaatta acccgcggat gttagatctc aatatgttgc tgttcagcac cagtgacttt
     5701 atctgcattg gcattgtcgc cctaccgctg acgatggtga ttgtcagtgg cgggatcgat
     5761 atttcgtttg gttcgaccat cggcctctgc gccattgcat tgggcgtact gtttcaaagt
     5821 ggtgtgccga tgccgctggc gattctcctg accttactgc tcggcgcatt gtgcgggctg
     5881 atcaacgccg gattaattat ctataccaaa gttaacccgc tggtgattac gcttggcacg
     5941 ctgtatctgt ttgccggaag cgccctgctg ctttccggga tggccggggc gacggggtac
     6001 gaaggtattg gtggattccc gatggcgttt acagatttcg ctaacctgga tgtgttgggg
     6061 ctccccgttc cgctgattat cttcctgata tgtctcctcg ttttctggct ctggctgcat
     6121 aaaacccatg ccggacgtaa cgtgtttttg attgggcaaa gcccgcgcgt ggcgctttat
     6181 agcgcgattc cagttaaccg taccttatgt gcgctctatg ccatgacggg gctggcgtct
     6241 gcggtcgccg ctgtgctgct ggtgtcgtat tttggttcag cacgttccga tctcggtgcg
     6301 tcgtttctga tgcccgccat caccgccgtg gtgcttggcg gtgccaatat ttatggtggt
     6361 tccggtgcca ttatcggcac cgccattgcg gttttattag tgggatattt gcaacaaggt
     6421 ttgcaaatgg caggagtgcc aaatcaggtg tccagcgccc tttccggtgc gctacttatc
     6481 gtcgttgtcg taggtcgttc cgttagcctg catcgccagc aaattaaaga gtggctggcg
     6541 cgtcgggcca ataacccatt gccataaagg atatcttcat gacacttcat cgctttaaga
     6601 aaatcgcctt acttagcgct cttggcattg ccgcaatctc tatgaatgtg caggccgcag
     6661 agcgtattgc atttattccc aaactggttg gcgtgggatt ttttaccagc ggtggcaacg
     6721 gcgcacaaca agcgggtaaa gagctgggcg ttgatgtgac ctacgacggg ccgacagaac
     6781 ccagtgtttc tggtcaggta cagttgatta ataacttcgt caatcaaggt tataacgcca
     6841 ttatcgtttc tgcggtttcg cctgatggct tgtgtccggc actgaaacgc gccatgcaac
     6901 gtggtgtgag agtgctgacc tgggactctg atactaaacc ggagtgccgc tcttactaca
     6961 ttaatcaggg aactcccgcc cagttgggag gtatgttggt ggatatggcg gcgcgtcagg
     7021 tgaataaaga caaagccaaa gtcgcgtttt tctactcaag ccccaccgtt acggaccaaa
     7081 accagtgggt gaaagaagcg aaagcgaaaa tcgccaaaga gcatcctggc tgggaaattg
     7141 tcactacgca gtttggctat aacgatgcca ctaaatcatt acaaaccgca gaaggaatat
     7201 taaaagcgta tagcgatctc gacgccatta tcgcccccga tgccaatgcc ctgcccgctg
     7261 ccgcacaagc cgcagaaaac ctgaaaaatg acaaagttgc gattgtcgga ttcagtacgc
     7321 caaacgtgat gcgtccatat gtggaacgcg gcacggtgaa agaatttggc ctgtgggatg
     7381 tggttcagca aggcaaaatt tcagtttatg tcgcggatgc attattgaaa aaaggatcaa
     7441 tgaaaacggg cgacaagctg gatattcagg gcgtaggtca ggtggaagtc tcgccaaaca
     7501 gcgtccaggg ctatgactac gaagcggatg gtaatggcat cgtactgtta ccggagcgcg
     7561 tgatattcaa caaagagaat atcggcaaat acgatttctg atgtgcatta cttaaccgga
     7621 gtaagttatg gcagatttag acgatattaa agatggtaaa gattttcgta ccgatcaacc
     7681 gcaaaaaaat atccctttta ccctgaaagg ttgcggtgcg ctggattggg gaatgcagtc
     7741 acgcttatcg cggatattta atccgaaaac gggtaaaacc gtgatgctgg cttttgacca
     7801 tggttatttt cagggaccaa ctaccggact tgaacgcatt gatataaata tcgccccgct
     7861 gtttgaacat gccgatgtat taatgtgtac gcgcgggatt ttgcgcagcg tagttccccc
     7921 ggcgaccaat aagccggtgg tactgcgggc gtcaggtgcg aactctatcc tggcggaatt
     7981 aagcaatgaa gccgtggcgt tatcgatgga tgacgccgtg cgcctgaaca gttgcgcggt
     8041 ggcggcgcag gtttatatcg gcagcgaata tgaacatcag tcgatcaaaa atattattca
     8101 gctggtcgat gccggaatga aagtgggaat gccgaccatg gccgtgactg gcgtgggcaa
     8161 agacatggtg cgcgatcagc gttatttctc gctcgcgact cgaatcgccg ctgaaatggg
     8221 gacgcaaatt atcaaaacct attatgtcga aaaaggtttt gaacggattg ttgccggatg
     8281 tccggtaccc attgttattg ctggcggtaa aaaattaccg gagcgcgagg cgctggaaat
     8341 gtgctggcag gctatcgatc agggcgcttc tggtgtggat atggggcgta atattttcca
     8401 gtctgaccat ccggtggcga tgatgaaagc cgtacaggcg gtggttcacc ataacgaaac
     8461 ggctgatcgg gcatatgaac tctatctgag tgaaaaacag taactgcgga tttaaggaga
     8521 agaattatgc acgtcacact ggttgaaatt aacgttcatg aagacaaggt tgacgagttt
     8581 atcgaagttt ttcgccagaa ccacctgggc tctgtacagg aagaaggcaa tttgcgcttc
     8641 gatgtcttac aggacccgga agtgaattcg cgcttttata tctacgaagc ctataaagat
     8701 gaagacgcag tggcgttcca taaaaccacg ccccactaca aaacctgtgt cgcgaaactg
     8761 gaatctttaa tgaccgggcc gcgtaaaaaa cgtctgttca atgggttgat gccgtgaggc
     8821 gaatttatca gttttatcta caattggggt aacgcgctga cgggagtaaa taaatgtctg
     8881 actggaaccc ctctttatat ctgcactttg ccgctgaacg atcgcgtccg gcggtggagc
     8941 tgcttgccag agtgccgctg gagaatgtcg attatgttgc cgatcttggc tgtggcccag
     9001 gtaacagcac cgccctgctg catcaacgtt ggcctgctgc caggataacg ggcatcgact
     9061 cgtctccggc gatgattgct gaagcgcgca gtgctttgcc agactgccag ttcgtggaag
     9121 cagacatccg caactggcaa ccggaacagg cactcgatct gatttttgct aatgcctcac
     9181 tgcaatggct gcccgaccac tacgaattgt ttcctcactt ggtttcttta cttaatccgc
     9241 agggcgtgct ggcagtacag atgccagata actggctgga gccgacccat gtgctcatgc
     9301 gcgaagttgc ctgggaacaa aactacccgg atcgcgggcg tgagtcgttg gctggtgttc
     9361 atgcttacta cgatattttg agcgaagccg gatgtgaggt cgatatctgg cgaaccacct
     9421 actatcacca gatgccgtcg caccaggcga ttatcgattg ggtgaccgcc actggattac
     9481 gtccgtggtt acaggatctg accgagagcg aacagcagct ttttcttacg cgctaccatc
     9541 agatgctgaa agagcagtat ccactgcaag agaacggaca gatactgctg gcatttccgc
     9601 gtctgtttat tgttgcccgc cgtacggagt aaatatcacg tcagttggta atggcgatcg
     9661 ggaagaactt ttgctggaat ttcggcttca tcgttcatct gtaagaggtc aatttcaata
     9721 gcgttacaga tggcatccag cggtaaatca ttgttttcag taccgaacgg atcttccagt
     9781 tcttccgcca gacaatccag cgaaataaaa gtataggaaa tcagcacaga gataaaaggc
     9841 gtcatgtaat gcaggtccac gaccagcgcg aacggcagca tgatacaaaa cagataaacg
     9901 gtacgatgca aaatcagggt gtaggcaaag ggaattggcg tgtaggcaat gcgctcgcag
     9961 cctgccagaa ccgctgaaat atcgttaagc cgatcgttga ggctaataaa caggatatct
    10021 gaaagttgtc cattgcgtcg ctgaaccgcc aaccactccc ccattattaa caagatgcgg
    10081 ttagctggag agttcgaagc cagtacacgc tgaagatctt cagtcttgag ataatgagcc
    10141 agcacttcca cctgtggctg tttgcgtaat gtcatgcgta aacagtgggc gaaggcgatt
    10201 tgcagccggg caaactccct tacacttgcc gaatccggca atgtcgtttt tacctcgcgc
    10261 agtaacgacc gtgaggcaat catcaactgc ccccaaagtt tgcgcgcttc aacgtaacgg
    10321 gcgtaaccgg cattattacg aaaaccaaga aaaatggcga tggcgacacc gagaatgctg
    10381 aacggtgcga gggtgaattt gatgcccaga tgcgtgtacc agggcagcat gaaaataaca
    10441 gcgatagaaa agagaaaatt gagtagtaag cgcgaggata tcttggataa tactgagcca
    10501 tgccagacaa aaatacggcg cagccagtgt tgttgtggac gaacaatcat ggttatcttc
    10561 aggcgtggaa aagtcgccct attaaacgtg attacgatca cattctcaag acactactta
    10621 caaattacct acaaaattac aacattaaca ggtgctgcat gtggcacctg ttaatgataa
    10681 aggcttatag gatgttgtaa ctaatataat gaaataagaa acccggtcga aaccgggttc
    10741 agagtattag cacaacggac gtacagcttc gcgcatccct ttttcgagaa tcgcatccag
    10801 gtcattagcc acctgctcga ccagaccagg cacttgcgtc aggtcctgct cccagtggtc
    10861 tttttctgcc aacacaatcg ctaccagttc ctgggtaccg ataacacggt cacgatgctg
    10921 actccagagt tgctgataac gttccagcca gtgtgcatcg tcctgtaccg gatatgtttc
    10981 tccattacgc tcaccgcgat agaacgcaat caatgccgct aatgcgaaag taaggcgcgc
    11041 cggaagtgtg ccgtttgcct tctgccctgc cagcagctgc ggcaggatgc gggtacggaa
    11101 cttggtcata ccgttgagcg cgatagacag cagctgatgc ttaatgtacg ggttacggaa
    11161 acgcccggtg actgcactgg cgaaagattc cagttcatcg cgtggcaaat ccagtaccgg
    11221 aataatttct tcgtagatgg ctttttcaac gaatgcgcaa atttcagcat cgttcatcgc
    11281 ttcacctacg gtatccagcc ctgcctggaa cgccaccggc accaacgcgg tgtgtgcacc
    11341 gttgagtatt gccactttgc gttctttata cggtttaata tcgtcaacaa tcagcacgtt
    11401 gagcggatat ttgtccagac gcagctcggt cgctaaggat ttcggtccct gaatcacaaa
    11461 caagtaaaag tgttcagcgg tgtcgaggaa accatcgtga taacccagtt cttcttccag
    11521 tttagccact tcatcacgcg gataaccggt aacgatacgg tccaccagcg tagaacagaa
    11581 gctgttagcc tgatccagcc attgaataaa tgcttctggc agtgcccact cttgcgcata
    11641 gcgcagcacc agttcacgca acgcgtcgcc attgtagtcg atcaactcac acgggatgat
    11701 gatccaacct ttatccagcg caccgttgaa atggctaaaa cgttcgaaca gcagacgggt
    11761 cagttttgcc ggataactta ccgctggcgc gtcatcgaat ttatcgcccg catggtagct
    11821 gatacccgct tcggtggtgt tagagaaaac aaagcgcatt tccgggttgt gcgccagttt
    11881 caggaattca tcgtattcac tgtagacgct gatttcacga ttaacagagc gaatcagacg
    11941 cgcgtcgctg accgcttccc ccttctcatt caggccacgg ataatggtgg tgtacagacc
    12001 atcctgcgtg ctcagtgacg gcgggaatga agtttcaatc ggacgaacaa cgaccacacc
    12061 agaattcaga tcggtgtgct cattcaggag atctatttgc cagtcaacaa aggcgcgcag
    12121 gaagttacct tcaccaaact gaatgatacg ttctggatac tgtgcaccgg gaaaatcgcg
    12181 acgatttagt gttttcacaa tgggttccct tctgattagt catacaacct gtttgaattg
    12241 gtacgacagg ttagcaaact ttaatacgac gaacccctgt tttgatcaac tcctgatgat
    12301 taatgagcag ttttatgaga aaagtgtggc gcggatcatg gtttaatcga ggaaatacgc
    12361 cttttcctgg atcataaagt ggtagaacac attgcattca aatcgcgcgg aatgaataaa
    12421 gatgtcagac aacttcctca ccgtaacgca tagtgctggt acggttgcgc ccatctttct
    12481 tcgagcgata cagacaggta tcggcttcga ccatcaattt attaaagtca tcggtcagcg
    12541 ttcggtacga tgcgcgacca ctaccgacgc caatacttac cgtgagataa agcgtttttt
    12601 gttgccaggt gaatggttgc agttcaacgc ctttacggat tttttccgcc attagcagac
    12661 catcgacagg attcactgac ggcactgcaa cagcaaattc ttcgccgccc atgcgcgcca
    12721 ccagcccctt atcaccgaca atcttctgaa tatgccgggc aaacacgctt aacactttat
    12781 cgccacattc atgtccatag ttatcgttaa tacttttgaa atagtcgata tcaagtagca
    12841 tgacagtcag atgttgtgtc tgtttcagcg atggactttt cagcgcctca taaagaccgg
    12901 accgtgagta aacctgagtc agaaaatcaa agtcggctcg cagcgcaact tgcttcatta
    12961 gcgaattgat cgctgccacg ctaaaagaaa ccataattgg gcatatcgcc atcgtggcaa
    13021 taccgagacg tgcggagaac atttgcggaa tggagaacgg cgaaccgacc gagatatcaa
    13081 tcaccgaatt agccaccagt acgatttcca ccgcaccggt gacaaaggtc aacagacatg
    13141 ttacctgcgg cgtatagcgc actgcacacc agattaatgc aggcagcggg aacgccagac
    13201 tccccgctcc gccaatgact accgaggcaa taaccgacac aataagcgcg atggcgggca
    13261 tcatctgttc tgctttaaag cgcggcagca ctccaggaat agccaacgtc agcatacaag
    13321 gcacgatcaa cacgcccgtt gagaattgct cactgaacca gtccgcaagc aaaggccaga
    13381 aatccagact atcaatactg accgaaccaa tcgccccgac aatagcgcat aataatgcgg
    13441 caatcagaca gtaattaaat agccgtaagg cacttactgg ctcatattta tttttcccca
    13501 aacgcttatc ccgcacgacc agtaaggcga cggtaacaat aaacatcata ttggagaaat
    13561 tgatagccag tgaaaccagt ccccattcgg tggttatggc atcataaacc agcatcgcca
    13621 cataactgat cgcatagtaa tgcagtcgat taagccagac atagcgggca aacacccccg
    13681 ccattacgcc attcagcggc cagaaaagag aaagttcgtg aaccagccgt aactctgcac
    13741 caataaaata gaacagcgtg gttagcgcaa aaatagcgat gctattacgt agcagatgac
    13801 cttcctggaa cacacggaaa gttgaaatgg ggtgtacatg cattaattat ctgtccatac
    13861 aattgcgggt ggcaattatc tcaacgtaat aagtaaattc tcataaagga tagcatgttc
    13921 gctgacagcg gcggcaaata aaggtaccct gggaatgcta ttttgtcgtt ttccgcaatt
    13981 ctcatggctg attcatctgt acaaccagcc agtgatagat taccgccacg atataacgtt
    14041 gttgttcttc gcttagcgaa acaccatgcg ggatgttatg ccacttagcc agacaaccgc
    14101 gacagcaggt cgcggtggcg tgctgagcga taaacaccgg atgcccgcgc atgggggttt
    14161 gcttaccgtc attagccggt aatgccggag caagacgttt agcgacaaaa tccgccgcat
    14221 gttgttcgat gactggcgcg cctttctcca gacaatactg acgctctttc atgcccaaac
    14281 gaaagcgaga tcgaaatttt gaacgggata aacgcgcgaa gagtggatcg agagactgca
    14341 ttaataaacc gaacgcccta actgtttcgt caattgttca aggacggcga tacccgcaag
    14401 cgagttacct gcatcatcca gttccggact ccagactgcg atggccattt catgcggaac
    14461 aatcgccaca ataccgccac caacgccaga tttcgccggt agtcccaccc gccaggcaaa
    14521 ctcccccgcg ttctggtaca taccactggt cgccatcagc gcgttaattt gccgtgcctg
    14581 cattggcgtc accacaggtt catcaatatg aatagctttc ccctgattag ccagaaagac
    14641 aaacgtccgg gccagctcta cacagctcat tttcagagcg cagtaatgaa aatagttttg
    14701 cagaacggtt gtcacgtcat gatggaaatt gccaaacgac ttcatcagcc aggcgatagc
    14761 cgcatttcgc gcggaatgtt caaattcgga acgcgctacc accgtatcgt aggaaatatc
    14821 agacacaccg cttaagccgc gcacgacttc cagcatacgt tgccgtggtg cgcttaatcg
    14881 cccttgcaac atatcgcaga ccaccagcgc accagcatta ataaacggat tacgcggaat
    14941 accctgctcc atttccagtt gcactaaaga attgaacggt gatccagacg gatctttgcc
    15001 gacgcgttgc cagatttcct cttcggagta atgacgcatg gcgacaacga gactcagcac
    15061 tttggaaata gactgaatgg aaaaacgttc ttgcgcgtct ccggcctgaa aaagctgtcc
    15121 gtcaacggta cagatagcaa tgcccaatcg ggaaccgtct actgtagcca gcgccggaat
    15181 ataatccgcg actttaccct gaccaatgag cggccgcact tgccgcaaga tgttttctaa
    15241 aattgcatta tccatggcga ctgccacttt ctactcctgg accgcaggtc tgaaaagacc
    15301 tgcgagtata tcagagctga atatgtcgcg tcagatccgg tctttccaca ccgtctggat
    15361 attacagaat tcgtgtaagc cgaaatggga aagctcacga ccaaagccac tctttttcac
    15421 gccaccaaag gccactcgcg cgtcgctggc acaataacca ttgataaaca ccccaccgca
    15481 ttccagacgt gccgccatct gtctggcctg tgtttcgtcg gtggtaaaaa tggtcgctga
    15541 aaggccgaac tcactatcat tagccagttc cagcgcatgt tctgcatctt tcgcaatggt
    15601 gattgccgca acggggccaa acatttcttc ccgaaaagcg gtcatttctg gggtaacatt
    15661 cgccagaacc gttggcggat agtagttacc tgccccagcc atcttttccc cgcccagtaa
    15721 caaacgcgca ccctgcgcca gggttttctc cacctgatga tgcagctcat cacgtaaatc
    15781 gaaacgagcc attggtccga gagcgttctc ttcgtcacgg ggatcgtcca ttttcaaggc
    15841 tgccgcagct gccacaaaac gttcggtaaa tgccgaagca attccctctt cgataataaa
    15901 gcgttttgct gctgcacaaa cctgtccggt attctgataa cgtccggcta ccgccgcttt
    15961 aactgccagt tccagatcgg catcgttaag cacaataaat ggatccgaac cgcccagttc
    16021 cagtacgcat tttttcagtg ccgctccagc ctgtgcacca atagccgctc cagcacgaac
    16081 acttccggtc accgtgatag cagcaatgcg cgagtcttta atcatctgag tgacaccgtc
    16141 attgtcggca ttcagccagc catatacgcc ttgtgggatt cccgcatctt taaacacctg
    16201 ggcaatgagc tgagcacagc ccatcacatt cggcgcatgt ttaagtaagt atccgttacc
    16261 agcaagaatg atgggaacag cgccacgcat cacctgccat aacggaaaat tccacggcat
    16321 aatcgccaga atcgtcccca acggtcgata ctcaataact gcctgctgat tttccaccag
    16381 cgtaggttcc gccttcagca ttgccggacc atgttctgca taccagtcac acaaattcgc
    16441 cgatttcgcc acttcagcgc gcgcctggtt gattggtttg cccatttcgc gggtgatcat
    16501 ttgcgccatt tcttcgctac gggcgcgcag agccttaccg atatcacgca gtttttcagc
    16561 acgataatct atatttgtct cgcgccagtc acgaaagcct gctgccgcca gctgaagtgc
    16621 gttttcgata tcatcagcgc cagcccacgg caacacagaa agttgttcac ccgtggcagg
    16681 atttatcgaa attgcatgag ttgccggagt aatggtcatc ggggtatctc ctttatgagt
    16741 catggtatga agatacgcag atttactctt gctttaaaat gaataatatt aagccactta
    16801 ttcacgaatc gagaatatta tggatctgac ccaactggag atgttcaacg cggttgccga
    16861 agctggcagc attacgcagg ctgcagcaat agtgcatcgc gtgccgtcga atctcactac
    16921 ccgtctacgc cagctggaaa cagaactggg ggtagatctg tttattcgcg agaatcagcg
    16981 tttacgtcta tctcctgccg ggcataactt tttacgctat agccaacaaa ttctcgcgtt
    17041 agtggatgaa gcgcggagcg ttgtcgctgg cgatgagccg caaggtttat tttctctggg
    17101 ttcgctggaa agcaccgcag cagttcgcat tccggccacg ctggcggaat tcaaccgtcg
    17161 ttatcccaaa attcagtttt cactttccac cggcccttcc ggcactatgc tggaaggtgt
    17221 gctggaggga aaacttaatg cggcgtttat tgatggaccc attaaccata ctgccattga
    17281 cgggataccg gtataccgcg aggagctgat gatcgtcacg ccacaaggtc atgcgccagt
    17341 aatccgtgcc agtcaggtta atggcagtaa catttatgcc ttccgcgcca attgttcgta
    17401 tcgtcgccac ttcgagagct ggtttcatgc tgacggtgcc gctccgggaa ctatccatga
    17461 gatggagtct tatcacggaa tgttggcctg tgtggtcgca ggagcaggta ttgcgcttat
    17521 tccgcgctct atgctggaaa gtatgccggg gcatcaccag gttgaagcgt ggccgttagc
    17581 tgagcaatgg tgttggctaa caacctggct ggtctggcgt cgtggtgcga aaacgcgtcc
    17641 gctcgaggca tttattcaac tactggatgt gcctgactcg acaaaacagg gttaccaatg
    17701 agctattttt gatagttctt gcggctaata tgctctatat agtgatgttc cgatgactta
    17761 tgactatatg gggcaaatat ggttacgcca gtaagcatca gcaattacat atctcttcct
    17821 gatgattttc ccgttcgtaa tattgcaccg caggtaaaag aagttttaaa ggattttatt
    17881 gatgcactta gtacaataat atgtaatgaa gagtggcgca cgtctttaaa catcaactca
    17941 gccacaaaaa agatatttaa taatcttgac aacttatcat atattcagag gacttctttt
    18001 cggggtaacg acacgctata caatgaaaag gttcagttta aactcactta tccgccag
//
LOCUS       DBFIFM010000086        17831 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.086, whole genome shotgun sequence.
ACCESSION   DBFIFM010000086 DBFIFM010000000
VERSION     DBFIFM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.086"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(110..250)
                     /gene="yjjY"
                     /locus_tag="N7C74_002505"
     CDS             complement(110..250)
                     /gene="yjjY"
                     /locus_tag="N7C74_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HGU2432353.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            346..1062
                     /gene="arcA"
                     /locus_tag="N7C74_002506"
     CDS             346..1062
                     /gene="arcA"
                     /locus_tag="N7C74_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HGU2432354.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1122..2474)
                     /gene="creD"
                     /locus_tag="N7C74_002507"
     CDS             complement(1122..2474)
                     /gene="creD"
                     /locus_tag="N7C74_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HGU2432355.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGIGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(2532..3956)
                     /gene="creC"
                     /locus_tag="N7C74_002508"
     CDS             complement(2532..3956)
                     /gene="creC"
                     /locus_tag="N7C74_002508"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HGU2432356.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPKSGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(3956..4645)
                     /gene="creB"
                     /locus_tag="N7C74_002509"
     CDS             complement(3956..4645)
                     /gene="creB"
                     /locus_tag="N7C74_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HGU2432357.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDIVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRSL"
     gene            complement(4658..5131)
                     /gene="creA"
                     /locus_tag="N7C74_002510"
     CDS             complement(4658..5131)
                     /gene="creA"
                     /locus_tag="N7C74_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HGU2432358.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5342..6211
                     /gene="robA"
                     /locus_tag="N7C74_002511"
     CDS             5342..6211
                     /gene="robA"
                     /locus_tag="N7C74_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HGU2432359.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6208..6855)
                     /gene="gpmB"
                     /locus_tag="N7C74_002512"
     CDS             complement(6208..6855)
                     /gene="gpmB"
                     /locus_tag="N7C74_002512"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HGU2432360.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6907..7419
                     /gene="yjjX"
                     /locus_tag="N7C74_002513"
     CDS             6907..7419
                     /gene="yjjX"
                     /locus_tag="N7C74_002513"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HGU2432361.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASIAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTTQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(7566..7892)
                     /gene="trpR"
                     /locus_tag="N7C74_002514"
     CDS             complement(7566..7892)
                     /gene="trpR"
                     /locus_tag="N7C74_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HGU2432362.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(7982..9919)
                     /gene="sltY"
                     /locus_tag="N7C74_002515"
     CDS             complement(7982..9919)
                     /gene="sltY"
                     /locus_tag="N7C74_002515"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HGU2432363.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10130..11797
                     /gene="ettA"
                     /locus_tag="N7C74_002516"
     CDS             10130..11797
                     /gene="ettA"
                     /locus_tag="N7C74_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HGU2432364.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(11904..12071)
                     /gene="nadS"
                     /locus_tag="N7C74_002517"
     CDS             complement(11904..12071)
                     /gene="nadS"
                     /locus_tag="N7C74_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HGU2432365.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVARHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(12105..13337)
                     /gene="nadR"
                     /locus_tag="N7C74_002518"
     CDS             complement(12105..13337)
                     /gene="nadR"
                     /locus_tag="N7C74_002518"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HGU2432366.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVVDGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEEDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13358..14740)
                     /gene="radA"
                     /locus_tag="N7C74_002519"
     CDS             complement(13358..14740)
                     /gene="radA"
                     /locus_tag="N7C74_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HGU2432367.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14789..15757)
                     /gene="serB"
                     /locus_tag="N7C74_002520"
     CDS             complement(14789..15757)
                     /gene="serB"
                     /locus_tag="N7C74_002520"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HGU2432368.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            15863..16504
                     /gene="ytjB"
                     /locus_tag="N7C74_002521"
                     /pseudo
     CDS             15863..16504
                     /gene="ytjB"
                     /locus_tag="N7C74_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
     gene            16535..17551
                     /gene="lplA"
                     /locus_tag="N7C74_002522"
     CDS             16535..17551
                     /gene="lplA"
                     /locus_tag="N7C74_002522"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HGU2432369.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGIPHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            17583..>17831
                     /locus_tag="N7C74_002523"
     CDS             17583..>17831
                     /locus_tag="N7C74_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2432370.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMT"
BASE COUNT         4237 a         4983 c         4497 g         4114 t
ORIGIN      
        1 gctagcattt agatacgatg atttcatcaa actgttaacg tgctacaatt gaacttgata
       61 tatgtcaacg aagcgtagtt ttattgggtg tccggcccct cttagcctgt tatgttgctg
      121 ttaaaatggt taggatgaca gccgtttttg acactgtcgg gtcctgaggg aaagtaccca
      181 cgaccaagct aatgatgttg ttgacgttga tggaaagtgc atcaagaacg caattacgta
      241 ctttagtcat gttacgccga tcatgttaat ttgcagcatg catcaggcag gtcagggact
      301 tttgtacttc ctgtttcgat ttagttggca atttaggtag caaacatgca gaccccgcac
      361 attcttatcg ttgaagacga gttggtaaca cgcaacacgt tgaaaagtat tttcgaagcg
      421 gaaggctatg atgttttcga agcgacagat ggcgcggaaa tgcatcagat cctctctgaa
      481 tatgacatca acctggtgat catggatatc aatctgccgg gtaagaacgg tcttctgtta
      541 gcgcgtgaac tgcgcgagca ggcgaatgtt gcgttgatgt tcctgactgg ccgtgacaac
      601 gaagtcgata aaattctcgg cctcgaaatc ggtgcagatg actacatcac caaaccgttc
      661 aacccgcgtg aactgacgat tcgtgcacgc aacctgctgt cccgtaccat gaatctgggt
      721 actgtcagcg aagaacgtcg tagcgttgaa agctacaagt tcaatggttg ggaactggac
      781 atcaacagcc gttctttgat cggccctgat ggcgagcagt acaagctgcc gcgcagtgag
      841 ttccgcgcca tgcttcactt ctgtgaaaac ccaggcaaaa ttcagtcccg tgctgaactg
      901 ctgaagaaaa tgaccggccg tgagctgaaa ccgcacgacc gtactgtaga cgtgacgatc
      961 cgccgtattc gtaaacattt cgaatctacg ccggatacgc cggaaatcat cgccaccatt
     1021 cacggtgaag gttatcgctt ctgcggtgat ctggaagatt aatcggcttt accaccgtca
     1081 aaaaaaacgg cgctttttag cgccgttttt atttttcaac cttatttcca gatacgtaat
     1141 tcatcgtccg ttgtaacttc tttactggct ttcattttcg gcagtgaaaa cgcataccag
     1201 tcgatattac gagtcacaaa catcatgccg gccagcgcca ccaccagcac actggttccc
     1261 aacaacagcg cgctatcggc agagttgagg agtccccaca tcacaccatc cagcaacaac
     1321 agcgcgaggg taaacaacat gctgttgcgc caacctttca ataccgcctg caaataaata
     1381 ccgttcatta acgccccaat cagactggcg attatccatg ccacggtaaa accggtatgt
     1441 tcagaaagcg ccagcaagag caaataaaac atcaccaatg aaagccccac cagcaaatat
     1501 tgcattgggt gtaaacgttg cgcggtgagc gtttcaaaaa caaagaacgc cataaaagtc
     1561 agcgcaatca gcagaatggc atacttagtc gcccggtcag ttaattggta ttgatcggcg
     1621 ggcgtcgtca ctgcgacgct aaacgcgggg aagttttccc agccggtatc attgcctgaa
     1681 gcaaaacgct caccgagatt attagcaaac cagctgcttt gccattgcgc ctgaaaacct
     1741 gactcactaa cttcccgttt ggctggtaga aaatcaccta aaaaactggg atgcggccag
     1801 ttgctggtta aggtcatttc gctattacgc cccgcaggca ccacagaaag atcgccggta
     1861 ccgcttaaat tcagtgccat attcagcttc aggttctgct tccgccagtc cccttcgggt
     1921 aaagggatat gcacgccctg cccgccttgc tctaacccga tgccgggttc aatagtcagc
     1981 gccgttccgt taacttcagg cgctttcacc acaccaatac cacgcgcatc cccgacgcta
     2041 atcacaataa atggcttgcc aaaggtgata tttggcgcgt cgagttcgct aagacgcgaa
     2101 acatcgaaat cggcttttag cgttaaatca ctgtgccaga cctgaccggt ataaatccct
     2161 atcttgcgtt cttccacgtt ctgattgcca tcaaccatca atgactcagg taaccaaaaa
     2221 tggataaaac ttcgtttccg ctccacggtt ttatcctctt cctgcaccgt ataaagctcg
     2281 gtcacaggaa tagcgataag aggcccaacg agtttttgcg gcccgctggt actttggcga
     2341 atcgcatctt ccacatcgct acggtaatcg gcacgttcga caatcacctg ccgaatcagc
     2401 attatcggaa tcaacaacag caatactgca ccaaacaggg tggtcatttt ccagaacagg
     2461 ggggatttca acatagtctt ctcctttgca atggcagcag gatacgaagg ctatgtggga
     2521 agaatttgaa gctatgtgaa gtgacggtga agccgaagcg aggccagcac gccaccttcc
     2581 tgcacgttgc gcagcgtgac ttcgccgtta aacaaacggg cgacctcact gacaaacgcc
     2641 aaccccagac cgctgctttt ttgcccattt gcacgcggca aagagtaaaa gcgttcaaaa
     2701 atacgtgaca gcgcgtagtc aggaatacca ctaccggtat ccagcacctt aagggcgacg
     2761 tgttcctgat ccacttcggc gcttagcgtt atgcgaccgc tcttgggggt aaaatcgatg
     2821 gcgttatcca gtaaattccc cagcgcctgc tccagtaacg ccggttcagc agcaacgtta
     2881 acctcagtag gcataacatg caaagtgatg tttttttctg ccaactgcac ggtgcgcgct
     2941 tcgctgacgc ggcgaaataa tgccgccaca tcaacagcag tcagaacgac ttcctgacga
     3001 ttctccagtc ttgcctggcg tagtaacgtt tctaccaatg cctgcatacg cgcattttgc
     3061 gtcagaatgt tgtcggtaaa acgagccacc acttccggtg gcggaccttc gcgtaaaatt
     3121 tccgccgcgc cacgaatcgc cgccagtggg ctttttagct catgggttaa cgcataaaca
     3181 tactgctcaa tatagttttt cccttccagc ttcacgcgca tactttccag cgcctgcgca
     3241 agcttacgca actcgctact gccgagttca ggaagaggaa cgggcttatt gtcagtgacg
     3301 gaatcggcat agcgagtgag ccgggcaatg gagcggttga tccaccaaac catgcctgcg
     3361 ccaatcacca gtgcaatccc cagcaaaatg gcgctggccc ataaaattcg ccgctcgctg
     3421 cgcttaatga ccggagccat cgccgcgttc ggtttgccta cgctcaaaac gccaataagc
     3481 cgcgagccgt ccataatcgg cgcggcaaca tacatcaccg aactttcggg atcggcagga
     3541 ttttgcaacg tgctgcgcgc accatactga ccacgcaacg ttagccagac gtcattccag
     3601 cgcgaataat cctgcccaac ggctttattt gccgaatcga acaatacttt gccctgcgca
     3661 tcggtcatat agacatgata ttcattgcgc actttgttaa tgccaccgat attagcgcga
     3721 aacgggcgat gttgtagctg attaaacgcc tgcgccagtt gcccatgcgt tgggtcccca
     3781 gagagcaaat cgggacgcgc cagctccgcc agcaacgttg cggtgtcgat cagcgtcccc
     3841 tccgttgctc ttcgcacgcc cggtttaact tctttgacaa aaatagccag tacgaaccag
     3901 gccgccaccg ccaccagtaa aaaatagccc agcaacaacc gcatgccgat acgcattaca
     3961 ggctcctcag gctgtatccc atgccgcgat gagtattaat cggtgaaaga tcggggttga
     4021 tggcgcgcag cttggcacgc agcgttttaa tgtgggtatc gacggtgcga tcgtaggtgt
     4081 cctgcgcatc ttcccacacg atatccatca gttgctggcg ggaccagacg cggcctggcg
     4141 acttgagtaa cgtcttcagc aataaaaact cataccgcgt cagcgttaat ggcgtgtcaa
     4201 accagctgat ctgcgccgcg ggttcattca attcaaaatg accaatacgg atgacgggag
     4261 acggcgtcga gaacttcttc acccgacgca gcaaggtgcg caccctagcg cacacttcgc
     4321 ggggtgaaaa cggtttagcc acatagtcgt cagcgccaat ttccagccca agcaggcgat
     4381 cgacctcttc acttcgggcc gtcaggaaca gtacaggtaa cgccggatgg agcgccagta
     4441 actggcggca caattcaaag ccactgatat caggcaaacc aacgtcgaga atcatgacat
     4501 cgggaacctg ctggcgagct ttatccagca ccggcaagcc tcgctcaaag acctcgacgg
     4561 caaaaccttc ctgctgcaac atgtagacca gcgtgtcggc tatcccttgc tcatcttcca
     4621 ctaaccagac cgtttcccgt tgcataaatc gcctctgtta ttgtcgccac ggcatgacag
     4681 gaaccgcgct aatcgcgttt ttcggcgaac cttctacaac tttgtcggag taagccagat
     4741 aagcgagcgc gttgcgtttg gcgtcataaa agcgcacgac ctgtaacgac ttaaagacca
     4801 gggacgtgcg ttttttgaat actacctcgc cctgagcttt gccgttttta atacgatccg
     4861 acagttcaat cggcccgact tgctgacaag aaatagccgc atcggaggta tcttccgcca
     4921 gacccaatcc ccctttaata ccacctgttt tcgcccggct cacataacag gtgacatttt
     4981 tcacatcggg atcgtcaaag gcttccacaa caattttgtg atccgggccg atcattttaa
     5041 atacggtgtc gaccgaacca atctcttctg catgagccaa tggccccagc attatcaggc
     5101 ttaaagacag gatcaaatgc ttgtatttca tattgttacc atttttatct attacacgag
     5161 caattagttc gtcacggtaa ataacatatt gattaagaac aaaatctcaa tacttttatt
     5221 tccgtcaaga cctaaaacat actctactaa gaaaaaaaca ctgaatgcta aaacagcaaa
     5281 aaatgctatt atccaattac ctgatgtcag gtgctcgttg ttgaaaggat gaggatattt
     5341 tatggatcag gccggcatta ttcgcgacct tttaatctgg ctggaaggtc atctggatca
     5401 gcccctgtcg ctcgacaatg tagcggcgaa agcaggttat tccaagtggc acttacagag
     5461 aatgtttaaa gatgtcactg gccatgctat tggcgcgtat attcgtgctc gtcgtttgtc
     5521 gaaatcggcg gtcgcactac gcctgactgc gcgtccgatt ctggacatcg cgctgcaata
     5581 ccgcttcgac tctcaacaga catttacccg cgcattcaag aagcagtttg cccagactcc
     5641 tgcactttac cgccgttctc ctgaatggag cgcctttggt attcgcccgc cgctgcgtct
     5701 gggtgaattc actatgccag agcataaatt tgtcaccctg gaagatacgc cgctgattgg
     5761 tgttacccag agctactcct gttcgctgga gcaaatctct gatttccgcc atgaaatgcg
     5821 ttatcagttc tggcacgatt ttctcggcaa cgcgccgacc attccgccag tgctctacgg
     5881 cctgaatgaa acgcgtccga gtcaggataa agacgacgaa caagaggtat tctataccac
     5941 cgcgttagcc caggatcagg cagatggcta tgtactgacg gggcatccgg tgatgctgca
     6001 gggcggcgaa tatgtgatgt ttacctatga aggtctggga accggcgtgc aggagtttat
     6061 cctgacggta tacggaacgt gcatgccaat gctcaacctg acgcgccgta aaggtcagga
     6121 tattgagcga tactacccgg cagaagacgc caaagcggga gatcgcccaa ttaatctgcg
     6181 ctgtgaactg ctgattccta tccgccgtta acgctgcagc tcatctaatg caggggcgtc
     6241 cagatgcgag atgtcccctg cggtttcaac gacccagcca gacgccagcc acaggctttc
     6301 ctgataatcc acgcgcgaaa tagaacagtt acgcagacgt aagcggcgct ctgcccatgc
     6361 tggtaatccg agaatcgtac tcaccagaca tcccagtgca ataccatggc tcaccagcag
     6421 cggtcggctt ccctgcggta agtcccggca agattccagt gccgcattga cgcgatcgct
     6481 gagctcctgc attgactcgc cttcaggaat acgcccatca acggtgccat tgaccagctg
     6541 ccgacgccag ttctcttctt cttcggtcag agaatcgata tgtctttttt ccagcacacc
     6601 catgtttaat tcacgcaggc gagaatcaaa gatgatgtca cagccgcagg cctgggcgat
     6661 gatttccgcc gtacgccggg tgcgtcctaa atcgctactg atgatatgcg taatgccaag
     6721 ctctttggca cgcgtcgcca cctgcatcgc ctgttgctca cctttggcgg tcagcgggct
     6781 gtcagactgg ccctgaatac gtcgctcggc gttccactgc gtttcaccgt ggcggactag
     6841 gtatacctgt aacatgcttt ttttccgtat actgctgtaa cgttaatttt ctgagtaatg
     6901 ctgattatgc accaagttgt ctgtgcgacc accaatcccg ctaaaattca ggccattctg
     6961 caggcatttc acgagatctt cggcgaagga tcctgccata ttgcatccat tgccgtcgag
     7021 agcggtgtac cggaacagcc ctttggcagt gaggaaacgc gcgctggcgc acgaaaccgg
     7081 gtagccaatg cccgccgttt acttccagag gctgattttt gggtggcgat tgaagctggc
     7141 attgatggcg acagcacttt cagttgggtg gtgattgaaa acaccaccca gcgcggcgag
     7201 gcgcgttcgg caactttgcc gttgcctgcg gtgatattgg aaaaagtacg tgaaggtgag
     7261 gcgcttggtc ccgtgatgtc gcgttatacc ggtattgatg agattggtcg taaggaaggg
     7321 gcgatcggcg tgtttaccgc cgggaaactc actcgcgcca gcgtctatca ccaggcggtg
     7381 atcctggcgc tgagtccgtt tcataatgcg gtgtattaag cgcctgatgc gacgctgccg
     7441 cgtcttatca tgcctaccaa acatattgaa attacgggta tttgtaggac ggataaggcg
     7501 ttcacgccgc atccggcatt cggtgcacga tgcctgatgc gccacgtctt atcaggccta
     7561 caaaatcaat cgcttttcag caacacctct tccagccact ggcgcagctc cacgggcgcg
     7621 gctttcaggc tgttagatcc acgcgtaatc gtcgcgatgc ctgcgccgag ttcatttttt
     7681 aactcacgct ggctcatttc gccgcgcaac agctcttcga caatacgcac gcgagtcccc
     7741 aacgcttcgc gctcatctgg cgtcagcatc aggtttaaca acggtaaatg gagatcgttt
     7801 tggtaggcat tcttaagcag gtcgacaaaa cgtaaccact cctggtgacg ctgttctgcc
     7861 atcgctgctg aatagggtga ttgttgggcc ataatatgtc gccattgtta gcgggggaag
     7921 caaaatgcct cccccggttg tactcgctaa agagtacgat agcatatcat aaacgtgcgg
     7981 atcagtaacg acgtccccat tccgtggcgc tcatcaacgt cggtttatcc cccatgaaat
     8041 agcggtagta agcgtcataa gccagcacgt tcttcacata accgcgcgtc tcggagaacg
     8101 gaatactctc gacaaatgcc acagcgtcga tacgcccggc gctgttgcca agccaggttc
     8161 gcacccgtcc aggtccggcg ttataagctg ctgaggagaa aatacggtta ttaccaaatt
     8221 gctgataaac atattgcagg taactggtgc caatgttgat attcgtttcc ggatccagca
     8281 attgcccagg gctgctataa ccaggaatag agaacatctt caccgtatgg gtcgccgtgc
     8341 ccggcataat ctgcatcagg ccgctggccc ctaccggtga tttcactttc ggattccagg
     8401 cgctctcctg acgggcaatc gccatcgcat agctttgcgg gatctccttc ccactggtgt
     8461 aacgtttgaa aagatcgttg taagccagcg ggaatcgctc ttccagatga tcccaaagct
     8521 tcccggcgat cgttgcctga acgctaagat cccaccattg gttgttgaaa gcataccgcg
     8581 ccagttgagc ctgctctgtt tttgacttgc tcttcaccag attggcccac tcgctacgcg
     8641 cggtgttatc gagattccag tacatcaact cgcgcacgcg cgccatctcc ggcccctgag
     8701 tcagggcgct gtcaacattc tgcggcgctt tatcaatctt cagctcatac tcttcgccga
     8761 tgcgttgtgc agcaaccatc gggtagaaac cacgctgttg catgagttga tgcaaaatct
     8821 cttttgcttc agcttcacgt ccgcgttcca gcaataaatc cgcctgccag taacgccatt
     8881 catctttctc tttcgcttcc ataggcagac gcgccagcca ggtattcagg ccgcgacgat
     8941 cgccagtgcc aagcgccatt cgcacacggc gttcaataag cgaagtagat tgcgagcgca
     9001 taatggcgtc atcgcgccat ttcgcctgct cgtcggtgac atcgttgccc atcaaacgcc
     9061 aggcgacgat atcgcgcagc tcctgaatct gatcttcatt aagctgctgc gcctgggcaa
     9121 gcgatgggat catcagccgt gcattttcag catcctgccg cgccacactg gcaaacgcca
     9181 ccgccgccat ttgacgggta aaatcggtcg ctccggttgt acgcgcgaag gtcagtaccg
     9241 tattggggtt gttcgccagt gaaatgattg ccgaggcgat agtctggtaa tcggcaggca
     9301 tctgccctgc cagcactgtt accaggccag tgttacccgc tttcatcgcc agacggatac
     9361 gctctaaata cgccagcgga tcttgtttac ctgacgcacg ccagacgcta aataacttgt
     9421 cacaggcgtt aggctggctc ttgccggtta gccacagctc tttcgcccct tgccaggctt
     9481 cttcactctg cccggtgttc catttcgcat agtagtaatt acattgtgct tcggtagttc
     9541 cgggcttttc cgggctaaag gctaacaggc cacgccagtc ttcacgccgc gccagttcat
     9601 tgacgaaacg agattgcagc gtgcgagcgg gaggaagcgt ggggttagcg cgaacaaagt
     9661 tagtgaccgt caccgccggt tgattcatca ggtcatcggt gatctggcgg tattccaggt
     9721 agggataaag cggataatcc ttcagtccag gcatcatttg ttcgaccaca tccatttgtc
     9781 gattatccca ggcctgcttg atttgcgcgt aacgactacg ctgctcatcc agtgagtcgg
     9841 ctcgcgccac gctgctgacc gtcagcagac agacaccggc agccaacagc cgccaggtaa
     9901 cttgtttggc tttttccaca agcgcatcct ctaagtgtaa atacatcaat gcagcgtcat
     9961 gccgcgtaat agatttatgc taaccagtca ttgccgttta cgccacgtta cggacacttt
    10021 tttactttta ctgcgagggt gatcgaccgg ctgggattag gcggggaaaa aggctacact
    10081 tcgcctttga aaactcatca tcacgataaa catagaggcg aagtccaacg tggctcaatt
    10141 cgtttatacc atgcatcgtg tcggcaaagt tgttccgccg aaacgtcata ttttgaaaaa
    10201 catctctctg agtttcttcc ctggggcaaa aattggtgtc ctgggtctga acggcgcggg
    10261 taagtccacc ctgctgcgca ttatggcggg cattgataaa gacatcgaag gtgaagcgcg
    10321 tccgcagcca gacatcaaga ttggttatct gccgcaggaa ccgcagctga acccggaaca
    10381 caccgtacgt gagtccattg aagaagcggt ttcagaagtg gttaacgccc tgaaacgcct
    10441 ggatgaagtg tatgcgctgt acgccgatcc ggatgccgat tttgacaagc tggccgctga
    10501 acaaggccgt ctggaagaga tcattcaggc tcacgacggt cataatctga acgtacagct
    10561 ggagcgtgcg gcggatgcgc tacgtctgcc ggactgggac gcgaaaatcg ctaacctctc
    10621 cggtggtgag cgtcgtcgcg tggcgttgtg ccgcctgctg ctggaaaaac cagacatgct
    10681 gctgctcgac gaaccgacca accacctgga tgccgaatcc gtggcctggc tggaacgctt
    10741 cctgcacgac ttcgaaggca ccgtggtggc gattacccac gaccgttact tcctcgataa
    10801 cgttgcgggc tggatcctcg aacttgaccg cggtgaaggt attccgtggg aaggtaacta
    10861 ctcctcctgg ctggagcaga aagatcagcg tctggcgcag gaagcttcac aagaagcggc
    10921 gcgtcgtaag tcgatcgaga aagagctgga gtgggtacgt cagggaacta aaggccgcca
    10981 gtcgaaaggt aaagcacgtc tggcacgctt tgaagaactg aacagcaccg aatatcagaa
    11041 acgtaacgaa accaacgaac tgtttattcc acctggaccg cgtctgggcg ataaagtgct
    11101 ggaagtcagc aacctgcgta aatcctatgg cgatcgtctg ctgattgatg acctgagctt
    11161 ctcgatcccg aaaggagcga tcgtcgggat catcggtccg aacggtgcgg gtaaatcgac
    11221 cctgttccgt atgatctctg gtcaggaaca gccggacagc ggcaccatca ctttgggtga
    11281 aacggtgaaa ctggcgtcgg ttgatcagtt ccgtgactca atggataaca gcaaaaccgt
    11341 ttgggaagaa gtttccggcg ggctggatat catgaagatc ggcaacaccg agatgccaag
    11401 ccgcgcctac gttggccgct ttaactttaa aggggttgat cagggtaaac gcgttggtga
    11461 actctccggt ggtgagcgcg gtcgtctgca tctggcgaag ctgctgcagg ttggcggcaa
    11521 catgctgctg ctcgacgaac caaccaacga cctggatatc gaaaccctgc gcgcgctgga
    11581 aaacgccctg ctggagttcc cgggctgtgc gatggttatc tcgcacgacc gttggttcct
    11641 cgaccgtatc gccacgcaca ttctggatta ccaggatgaa ggtaaagttg agttcttcga
    11701 aggtaacttt accgagtacg aagagtacaa gaaacgcacg ctgggcgcag acgcgctgga
    11761 gccgaagcgt atcaagtaca agcgtattgc gaagtaatgc gtaaaatgcc ggatgcggcg
    11821 cgaacgcctt atccggccta caaaaccagc aatttcaata cgttgtaaaa ctgtagacct
    11881 gataagacgc ggtaagcgtc gcatcaggca ttgagcaccg aatgccggat gcggcgtgaa
    11941 caccttttcc ggtctacaac agccttagca tcctcaattt cgtggcgggc cacccgtgca
    12001 ggttttttca aaccttgttt aatctcgaca gcctcttcca gagaggtttt caactcatca
    12061 aaaaaactca ttttatacct cgcctttgag cagtttcatc acggttatct ctgctccccc
    12121 atcatctccc gcaccagctc cacgcagcgc aggaaacggc tgtcgtaatc ttcctcttca
    12181 acccgcacaa attcgatatt gttctcttcc agcatctcca ccagcagatt ctggaactct
    12241 ttacgatcca ccgaactgcc gaggctgcgt aaaccatcca ccacccacgg cgtgttgttc
    12301 tccagcagga tcaccagatc gaaacggtat tcatcaatca gcgcctgcac gaacggatgt
    12361 tcccgccctt cgtacttttt gcagaacgcc tgagtggtga caaaatcggt atcgataaat
    12421 gccactttat tggcatattt caccgcaaaa tcaatgtatt gagcgtggcc cagcgcgatt
    12481 ttatcgtagt cagaatactg caatgcgatc tcatcaccgc cgaggtgtga aaagacataa
    12541 tcgcggccat attcccacgc actggtggtg ttgaagatat tggcaagttt gtttaccagg
    12601 gtggatttac cgctcgactc gccgccaagg atcgccacgg tacgcacaaa aaacggcttc
    12661 acttcggtag gaatatattc ccagtagcgg aacgggtttt cgcggatctg cgcaccgctg
    12721 atactcataa aggtacgttt cggatcgacc agcaccgtct cgatccccag atgttccata
    12781 tactgtggcg catcggcttc ttccgaggtg tagatcagat ccggctggat ccccttttca
    12841 gccataaact ttttgatacc gttgctccac acatcccagc cgtgcggata cggctccatg
    12901 ccctcttcgt tgaaagcatg aatgcgaata tttttctgat atttaaaagt ttgcaataac
    12961 cagcgcagac gatccggcac ggtcggctgc tgcgacatgg cactgtcttc gaacaacgcg
    13021 cggtcacggg tatcgtcaaa acccataatg atatgcagct cgtcaacctg gctacaggcg
    13081 cgctggataa ggtagatatg tccggtatgc agtgggtaga acttaccgaa tacgacaccg
    13141 atcgttttct tctgccgggg aaactcaagc cccaaaaaac ggtgcaacgc ctccagcttt
    13201 tgcgcgctgg ggcttttgat tttggcattc agtaactggc ttaaataccc tttggtcata
    13261 ccgctggcat cagctacctg ctgtagcgtg cagccctgtt gcttgatggc agttttcagg
    13321 taatcaaatg acgacatatc tccctccgta tttctcatta taagtcgtcg aacacgctaa
    13381 gcgcgtcgga gagtttttta acgccaaaaa tctgcatccc ttccggcgct tttttcggta
    13441 cgttagccgc cggaacaatc gcccggcgaa aaccgtgttt cgccgcttca gagattcgtt
    13501 cctgaccgct gggcaccggg cggatctccc ctgccagccc gacttcacca aacaccacca
    13561 gatcctgcgg tagcggtctg tcacgcaggc tggaaaccat cgccagcagt aacgctaagt
    13621 cggcgctggt ttcggttacc tttacgccgc cgaccacgtt cacaaacaca tcctgatcgg
    13681 ccatttgcag accaccgtga cggtgcaaga cagccagcag gattgccaga cggttttgtt
    13741 ccagccccac tgccacgcgg cgcggattcg ccatcatcga gtgatcgacc agcgcctgaa
    13801 tctccaccag caacggacgc gttccttccc acaccaccat cacggagctg ccggaggtca
    13861 cttcatctcc gcgacttaag aaaattgccg aagggttgct gacttcacgc agcccctgtt
    13921 cggtcatcgc gaagacgccc agctcattca ccgcgccgaa gcggttttta tggctgcgca
    13981 aggtgcgaaa acgggagtcg gcatcgccat ctaaaagcac cgaacagtcg atacagtgtt
    14041 ccagcacttt cgggccagcc agcgaaccat ctttggttac gtgccccacc atgacaatcg
    14101 ccacaccgcg cgttttggcg aagcgtgtca aataagccgc cgtttcacgc acctgcgcca
    14161 cgctaccagg cgatgactgt acatccgcca tatgcatcac ctggatcgag tcaattacca
    14221 tcagcttcgg ttgctcttct tcggcaatca ggcagatctg ttcgatgctg gtttccgaca
    14281 acatattgag attgtcagtc ggcaggccaa ggcgatgagc gcgcattgcc acctgttgca
    14341 gcgactcttc gccggtgaca tacagcgttt tcatctgctg ggccagtttg cacagcgttt
    14401 gcagtagcag cgtggatttc cccgcaccag ggttaccgcc aatcagaatg gcacttcctg
    14461 gcaccacgcc gccgcctagt acgcggtcga actctttaaa tccggtggaa aaacgcggca
    14521 gctcttcaag gctgatatcg gagagtttct ggacttttgc caccccggcg ctaccggcat
    14581 agccgctgag acgctcgtta cgcgccaccg taggcgacgc agcaagacgc acctcggtga
    14641 tggtgttcca ggcatgacag gcactgcact gcccctgcca gcgcggataa tcggccccgc
    14701 attcattaca aacaaaggcg cgttttggag cttttgccac ggtgtacctc gttaatgctg
    14761 tgccgcccgc aggatggcgg gcgagcaatt acttctgatt caggctgcct gagaggatgc
    14821 agaatacccc catcaggtca gcgtgacgga tggtgacttc cgccttttca ttcacttttg
    14881 gcttggcatg gtaggcaatc cccagccctg ccgctttgat catcggcagg tcattggctc
    14941 catcgccaat cgccacggtc tgcgccagcg ggatttcata ctcctgcgcg aggcgagtca
    15001 gagttttcgc tttgtactgc gcgtctacga tgtcgccgat cacattgccg gtaaatttac
    15061 cgtccatgat ctccagttca ttggctacca cggctgtcag gcgcagcttg tcgcgcaggt
    15121 attcagcaaa gaaagtaaag ccgccggagg caatcgccac tttccagccc agcgtttcca
    15181 gcttgagcac cagttgcgtt aagcctggca tcagcggcag attttcacgc acctgttgca
    15241 gaatattggc gtcagcgcct ttcagcgtcg ccacgcggct gcgcaggctg gcggtaaaat
    15301 cgagttcgcc gcgcatcgcc cgttcggtta cttccgccac catctcgccc gttccggcca
    15361 gtttggcaat ttcatcaata cattcaatct ggatggcggt ggaatccatg tccatcacca
    15421 gcaaacccgg cgtgcgcagg tgcgggattt ttcccagcgg cgcgacatcc agctgcgctt
    15481 cgtgggccag gcgtgtagcc cgtgcggtga gtgaacctgc cagacgaatc acctgataat
    15541 cttccacgca ccaggcggca acaatcacca tcgccgcacc cagtttgctc tggtattggg
    15601 tcagacgctg tttatccagc ccacgaccat acagcagcca gccgctacga cctgcgtggt
    15661 aatccagtgg catcacttca tcaccactta atgaaagagg cagacccggc cataaagaga
    15721 catcttcagg caggtcgcac caggtaatgt taggcattaa ggctcctgta aaatcgttcg
    15781 aagcagggaa aataacgcat gaggctacct tgtatccatt gcttctggca acattaagtc
    15841 tcaaattttc aaagggtgga agatggctcg cacaaaactg aaattccggc tgcatcgggc
    15901 agtgattgtc ctgttctgtc ttgccttgtt agtggcactg atgcaggggg cgtcatggtt
    15961 tagtcaaaac caccagcgac agcgtaatcc acagctggaa gaactggccc gcaccctggc
    16021 gcgtcaggtg acgctgaacg ttgcaccgct gatgcgtacc gactcaccgg atgaaaaacg
    16081 cattcaggcg atcctcgatc agttaacgga tgaaagccgt atcctcgacg cgggtgtgta
    16141 tgacgaacaa ggcgatctta tcgcacgttc tggcgaaagc gtcgaagtgc gcgatcggct
    16201 ggcgctcgac ggtaaaaaag caggcggcta ttttaaccag cagattgtcg agccaattgc
    16261 cgggaaaaac ggaccgctcg gctatctgcg cctgacactc gacacccata cgctcgccac
    16321 cgaagcccaa caggtggata acaccactaa cattttacgc ctgatgttgc tgctctcact
    16381 ggcaatcggc gtagtgctga cccgcacgct gctacagggt aaacgcaccc gctggcagca
    16441 atcgcccttc ctgttaaccg ccagcaaacc ggtgccggaa gaggaagaaa gcgagaaaaa
    16501 agagtgcccc attactacaa gaaaggaaat cgttatgtcc acattacgcc tgctcatctc
    16561 tgactcttac gacccgtggt ttaacctggc ggtggaagag tgtatttttc gccaaatgcc
    16621 cgccacgcag cgcgttctgt ttctctggcg caatgccgac acggtagtaa ttggtcgcgc
    16681 gcagaacccg tggaaagagt gtaatacccg gcggatggaa gaagataacg tccgcctggc
    16741 gcggcgcagt agcggtggcg gcgcggtgtt ccacgatctc ggcaatacct gctttacctt
    16801 tatggctggc aagccggagt acgataaaac catctccacg tcgattgtgc tcaatgcact
    16861 gaatgcgctc ggcgtcagtg ccgaagcgtc cggacgtaac gatctggtgg tgaaaaccgc
    16921 cgaaggcgac cgcaaagtct caggatcggc ctatcgcgaa accaaagatc gtggcttcca
    16981 ccacggcacc ttgctgctca atgccgacct tagccgcctg gcaaactatc tcaatccgga
    17041 taaaaagaaa ctggcggcga aaggcattac ttcagtgcgt tcccgcgtga ccaacctcac
    17101 cgagctgctg ccggggatcc cccatgagca ggtttgcgag gccataaccg aggccttttt
    17161 cgcccattat ggcgagcgcg tggaagcgga aatcatctcc ccggacaaaa cgccagactt
    17221 gccaaacttc gccgaaacct ttgcccgcca gagtagctgg gagtggaact tcggtcaggc
    17281 tccggcattc tcgcatctgc tggatgaacg ctttagctgg ggcggcgtgg aactgcattt
    17341 cgacgttgaa aaaggccata tcacccgcgc ccaggtgttt accgacagcc tcaaccccgc
    17401 gccgctggaa gccctcgccg ggcgactgca aggctgcctg taccgcgcgg atatgctgca
    17461 acaagagtgc gaagcgctgt tggttgactt cccggaccag gaaaaagagc tacgggagtt
    17521 gtcgacgtgg atagcggggg cggtaaggta actcgctgat taaaatatcc aaggaaggaa
    17581 atatgatcga tctggaaaac caggaacggg agattatcaa tttaatgttc agccagcgta
    17641 tttcctggct agccgccgtg cgtatcaggc ataaattatc gcttgctgaa gtaagtaaaa
    17701 tgttaggtat ctcgattaat tctctaaaac agatcgaaaa gacggaacgg cttagctcaa
    17761 acatcaaaag caaaatggca gaaatttatg gttgtccacc agagttgctg atatgccctt
    17821 cctggatgac g
//
LOCUS       DBFIFM010000087        17776 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.087, whole genome shotgun sequence.
ACCESSION   DBFIFM010000087 DBFIFM010000000
VERSION     DBFIFM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.087"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            91..594
                     /locus_tag="N7C74_002524"
     CDS             91..594
                     /locus_tag="N7C74_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HGU2432371.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKKFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(661..2118)
                     /gene="nuoN"
                     /locus_tag="N7C74_002525"
     CDS             complement(661..2118)
                     /gene="nuoN"
                     /locus_tag="N7C74_002525"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HGU2432372.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2125..3654)
                     /gene="nuoM"
                     /locus_tag="N7C74_002526"
     CDS             complement(2125..3654)
                     /gene="nuoM"
                     /locus_tag="N7C74_002526"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HGU2432373.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3885..5726)
                     /gene="nuoL"
                     /locus_tag="N7C74_002527"
     CDS             complement(3885..5726)
                     /gene="nuoL"
                     /locus_tag="N7C74_002527"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HGU2432374.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5723..6025)
                     /gene="nuoK"
                     /locus_tag="N7C74_002528"
     CDS             complement(5723..6025)
                     /gene="nuoK"
                     /locus_tag="N7C74_002528"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HGU2432375.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6022..6576)
                     /gene="nuoJ"
                     /locus_tag="N7C74_002529"
     CDS             complement(6022..6576)
                     /gene="nuoJ"
                     /locus_tag="N7C74_002529"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HGU2432376.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6588..7130)
                     /gene="nuoI"
                     /locus_tag="N7C74_002530"
     CDS             complement(6588..7130)
                     /gene="nuoI"
                     /locus_tag="N7C74_002530"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HGU2432377.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7145..8122)
                     /gene="nuoH"
                     /locus_tag="N7C74_002531"
     CDS             complement(7145..8122)
                     /gene="nuoH"
                     /locus_tag="N7C74_002531"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HGU2432378.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8119..10845)
                     /gene="nuoG"
                     /locus_tag="N7C74_002532"
     CDS             complement(8119..10845)
                     /gene="nuoG"
                     /locus_tag="N7C74_002532"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HGU2432379.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQCRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10898..12235)
                     /gene="nuoF"
                     /locus_tag="N7C74_002533"
     CDS             complement(10898..12235)
                     /gene="nuoF"
                     /locus_tag="N7C74_002533"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HGU2432380.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12232..12732)
                     /gene="nuoE"
                     /locus_tag="N7C74_002534"
     CDS             complement(12232..12732)
                     /gene="nuoE"
                     /locus_tag="N7C74_002534"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HGU2432381.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDAAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHANLTPEAIPE
                     LLERYK"
     gene            complement(12735..14537)
                     /gene="nuoC"
                     /locus_tag="N7C74_002535"
     CDS             complement(12735..14537)
                     /gene="nuoC"
                     /locus_tag="N7C74_002535"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311197.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HGU2432382.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPARVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14631..15293)
                     /gene="nuoB"
                     /locus_tag="N7C74_002536"
     CDS             complement(14631..15293)
                     /gene="nuoB"
                     /locus_tag="N7C74_002536"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HGU2432383.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15309..15752)
                     /gene="nuoA"
                     /locus_tag="N7C74_002537"
     CDS             complement(15309..15752)
                     /gene="nuoA"
                     /locus_tag="N7C74_002537"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HGU2432384.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16383..17321)
                     /gene="lrhA"
                     /locus_tag="N7C74_002538"
     CDS             complement(16383..17321)
                     /gene="lrhA"
                     /locus_tag="N7C74_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HGU2432385.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPISAPEGDDSLLIERDIE"
BASE COUNT         4413 a         4977 c         4616 g         3770 t
ORIGIN      
        1 gaggcgggtc cagaaacagc gacatgtaca ttttattttc cctcgtctgt tttaagatgc
       61 gaaacataaa attcaatgga ggaaaaataa atgggaatga ttggttattt tgcagaaatt
      121 gactcagaaa agattaacca actgcttgag tctactgaaa aacctttgat ggataatata
      181 catgacacgc tttcaggatt acgccgactg gatattgata aacgctggga ttttttacac
      241 tttgggctga caggcacctc tgcgttcgat ccagcgaaga atgatccgct gagtcgtgcc
      301 gtgcttggtg aacacagcct cgaagatggt atagatggtt tcctgggatt gacatggaat
      361 caggagttag ccgcgacaat cgaccgactg gaatcactgg atcgaagtga gctgcgtaaa
      421 aagttttcca ttaagcgact caatgaaatg gagatttatc cgggagtgac cttttcagaa
      481 gagttagagg ggcaactctt tgcttcgatc atgcttgata tggaaaagct gattagcgcg
      541 tatcgacgta tgctgcgaca agggaatcat gcgctgacgg ttattgttgg gtgatggtgt
      601 cagggcacca aaaaaaaccg ccgaacatgt cggcggcttt ctgacttaca aactgatagc
      661 ttacatcagc ggcattgcta accgcacaat actaatcagc ggttgtggcc atacacccag
      721 caccagtacc aacagtgcag aaatcagcac cacgataccg cccgcgctgt actgccagtt
      781 tgacggtgca tcgcgacccg gttgttccgg tgcgtgcagg tacaggctca ccgccacgcg
      841 caggtagtag tagaggccga ttgccgaacc gacaaccacg gcacccacca gccaccacag
      901 gtgtgcctgg acaccaaccg ccagcacgta gaacttaccg ataaagccca gcgtcatcgg
      961 gataccggcc agagacagca tcatcaccgt catcaccgcc gcgaggatcg gacgatgcca
     1021 gaacagaccg cggtaagaga acagggaatc agcatccggg ccacgatacg ggctggacat
     1081 caggctgacc acgccgaacg cgccgaggct gctgaacaga taaccggcca ggtaaacccc
     1141 taccgcttcc atcgacatct cgccagtttg cagcgcaatc agcgccacca gcagatagcc
     1201 gaggtgagag atagatgagt aaccgagcag acgtttgata ttggtctggc tcagcgccat
     1261 caggttaccg aagatgatgg aggcaaaggc gataatcgcc agcaccaccc gaatcgcttc
     1321 gctgtcaccc accggtgcgt agaggaacag acgcatcacc acaccgaaga tagcgatttt
     1381 gctcgccgtc gccaggaaag tggaaaccgg cgcaggcgcg ccctggtata cgtctggcgt
     1441 ccacaggtgg aacggcacca gagagagttt gaagccgagg ccaacaatca tcaggccgaa
     1501 acctgccagc aacagcggct cgtttagcat accgtcgccg aggtttttac ccagcgcgac
     1561 aaacgacagg tcgccagact gcgcatacac cagcgccata ccgaacagca ggaaagaaga
     1621 cgctgcggca gaaaggatgg tgtatttgat actggcttcc agcgaacgtt tctggcggaa
     1681 agcgtaaccg accaggccaa acagcggcaa agagatcagt tcaataccga ggaacagaga
     1741 cgccagatgg tttgcattcg ccagcaggat cccgcccagc gcggcaatta acaccaacag
     1801 gtagaactca tccttgttgt cgttatagcc ttcaagccac gggtaggcga aagtacaggt
     1861 ggcgaggctc gccaacaata ccagcccggt gtaaagcatg gcgaaaccat caacgcgcat
     1921 cagcggcgta acgtccatag cgcccgcctg gccaacaaac cagagcgaaa ccagcgccgc
     1981 gttaagccca ataaccgaga gcgtagcgtt gaggaaatga ttgcgtcgcc acgcaatgga
     2041 gagcatcaca accaccaccg tcaagccgac gatcagcaac ggtagcagtg cgatcaggtt
     2101 ttgtggagtt attgtcatgg cgatttacgg ccttgtagta gtaacggaat taacaaacca
     2161 ctgctggata ttgccaatcg cggagtgcga ggtatccaga atcggctgcg gatagaagcc
     2221 cagcagtacc agcagcacca ccagcaacag gatcataaac agctcacgca gcgacatccc
     2281 tggcagttcc tggctggcaa tctggctttt cgctttaccg aagtaagcgc gatgtaacat
     2341 cgccagcgaa taaacagatg caaagaccag cccaaaggta gagataacgg taatcaccgg
     2401 gacaacctgg aagctgccga acagaatcat aaattcgccg acgaagttac cggtgcctgg
     2461 catcccgagc gttgccaccg caaagaacag tgacaaggct ggcagccatt tcatcttgct
     2521 ccacagaccg cccatcatgc gcatgtcgcg ggtatggata cgttcataaa gctgaccaca
     2581 aagaataaac agacccgccg ctgacaagcc gtgcgcaatc atctggatta ccgcgccctg
     2641 gtaggccaac tggctgccgg tgtagatagc aatcagtacg aagcccatgt gggaaaccga
     2701 ggtgtaggcg atcagacgtt tgatatcggt ctgggcgaag gccatccacg caccgtagaa
     2761 gatgccgata acacccagcc acatggcgat tggcgcgaac tctgccgacg cgtttgggaa
     2821 cagcggtagg gagaaacgca gcagaccgta agcggcggtt ttcagcaaga tccccgcgag
     2881 gtccacggaa ccggcggtcg gtgcctgtga gtgcgcatcc ggcagccagc catgcagcgg
     2941 aaccaccggc attttcaccg caaacgcgat gaagaagccc agcatcaaca gatattccac
     3001 accactggac attggcgtat tcagcagctc ttcatagttg aaggtccaga cgccggtcgc
     3061 attgtagtga acaaaaacca gcgccaggat ggcgatcaac atcaccagac cactcgcctg
     3121 ggtgtaaatg aagaacttgg ttgccgccgt gatacgcgtt ttaccatcag aggctttatg
     3181 cccccacagt gcgatcagga agtacatcgg caccagcatc atttcccaga agaagaagaa
     3241 caggaacatg tcgatggcaa ggaacacgcc gataacgccg cccaggatcc acatcaggtt
     3301 gaggtggaag aagccctgat atttttcgat ctctttccac gaacagagta ccgccagcac
     3361 accgagcaga ccggtcagca cgaccatcag cagcgacagc ccgtcaatgg cgagatggat
     3421 agagatacca aaacgcggga tccacggcat gtcgaattca gactgccact gcggaattcc
     3481 ggcggattgc gtcagtgaat aaccgccctg caaccacagt tgcagcgaca gcgccagcgt
     3541 caatcccatg gtgatcagcg cgatccagcg cggcaccttg acgccaaagc gttcggtctg
     3601 ccagcacagg aagccgccga taaaggggat tagtattaac cagggtaata acatagctaa
     3661 gtatgtccct tatatttact cctgctcagg ctatctcact cgccagcccg aatcccggca
     3721 gtgctcgcaa tcctcacgta cttaagtacg ctccggttgc tgtgcgctgg cggtatccgg
     3781 tctgacttcg ccgatgacgc ctgtacttca ggaacagtta cctaaaattt attcttagtt
     3841 cacagacccg gcgcaacgcc gggtatattc actcagccaa caactcaacg cagtaccatc
     3901 aacagtgcca gcaccacgac cgcaccgatg ctcatggatg ccacatacca gcgcagataa
     3961 ccgttctcgc ttaacagcag gcctttacct gcaaagcggg aaaggacagc cgggatgttc
     4021 atcattgagt tcagcggatc gcgtttcagc aaccaggcaa tacccaggaa cggcttgacg
     4081 aacactttgt cgtacagcca gtcaaagccc caggcgttgt accaccaggt gcccagcaga
     4141 cggcccggcg cactgttggc gatggaggtc accagagtac gtttacccag ccacagccag
     4201 gctgccagca gaatgccgac caccgcgacc acgccagagg taatttccag ggtcaacatg
     4261 ctgccgtgcg ccagttccgt cgtttgcgga agcacgccct gcagcggcgg tacaatcagt
     4321 gcgccaacga aggtggaaag gatcagcagc acaatcagcg gcaggctgtg agttacccct
     4381 ttcacggcgt gagcgtgaat ttgttctttt ccgtggaaga cgatgaaaat catacggaag
     4441 gtgtagagcg aggtcataaa cgcaccgacc agacctgcca ccatcagatt gatatgacca
     4501 ttcgccatcg cacccgcgag gatctcatcc ttactgaaga agcccgcagt gactagcggc
     4561 agtgccgaca gtgctgcgcc gcccaccagg aagcagagat aaaccagcgg aatagattta
     4621 cgcagaccgc ccatcttgaa gatgttctgt tcgtgatggc aggcgagaat gacggaaccg
     4681 gatgccagga acagcagcgc tttaaagaac gcgtgggtca tcaagtgaaa aatcgccgca
     4741 tcccatgcct gcacgccaag cgcgaggaac atgtagccaa tctggctcat ggtagagtaa
     4801 gcgagaacac gtttgatgtc ggtctgtacc agcgcggcaa aaccggccag cagcagcgta
     4861 accgccccaa caatgcccac cagatgcaga acttccggcg tcatcaggaa caggccgtgg
     4921 gtacgggcga tcaggtagac acccgcggtt accatcgttg cggcgtggat cagcgcggag
     4981 acaggcgtcg ggcccgccat cgcgtcggca agccatgtct gcaacggcaa ctgcgcagat
     5041 ttaccgaccg caccgcccag cagcatcagc gtcgcccaca tcagcatgtt attgccgtca
     5101 gcaaagtgcg ctggtgccag ttccaccatt tcgcggaagt tcagggtgcc cagttcgttg
     5161 taaagaatga acagcgcgaa agcgaggaac acgtcaccca cacgggtcac gacgaacgct
     5221 ttcattgccg ctgcgccatt cttcggatcg gtgtaataga acccgatcag cagataggag
     5281 cacaggccca cgccttccca gccgaggtac atcagcagca ggttgtcggc aagcaccaga
     5341 accaccatgc tggcgataaa caggttggtg taagcgaaga agcgagagta gccctcttca
     5401 ccgcgcatat accaggaggc gtacatgtga ataaggaaac ccacaccagt gaccaccgag
     5461 agcatggtca gcgacaggcc gtccagcacc aggttaaaac cgatgttaaa gtcgcctacc
     5521 gacatccacg tccacagcgg ctggctgtat gcctgctcgc cgttagcgaa gaagtcaacg
     5581 ccaataaagg cagttaccag cgccgccagg cccacagagc caacgccgac gatcgccgag
     5641 acgttttcag accagcgccc acgggagaac gccagcagga cgaagccaat caatggcaaa
     5701 ataatggtta aggcaagcat gttcatccgc gcatctcact tactgaatcg atgttcaggt
     5761 tctggcgacg acggtgaagt tgcagcagca gcgcaaggcc gatactcgct tctgccgccg
     5821 cgaggctgat ggcgagaatg tacatcacct gaccgtcggt ctggccccag taacttccgg
     5881 cgaccacaaa ggccagcgcg gaagcgttaa tcatgatttc cagaccaatc agcataaaca
     5941 gcagattgcg acggataacc agaccggtta agccaagaac gaataagatt gccgcgagga
     6001 tcagtccatg ttgtaagggg atcatgcgtg ctcctccgtt tttcttttcg cgctgtcgtc
     6061 tttacgattg ctcagcactt cacccgcacg ctcttcacga ccgacgtgga aggccacaac
     6121 cagacccgcg agcagcagca tagaagccag ttccaccgcc agtacgtaag gcccgaacag
     6181 cgtaataccc actgctttag cactgattgg cgtaccgtcg ataccttgat cgttaacacc
     6241 gagaatggcg taaacaatca ccaccaacat gatggctgac aaaattgccg gaccaatcca
     6301 cacctgcggt ttcagccact ggcgttcttg ttcgatttct gaaccgccca ggttgagcat
     6361 catcaccacg aacacgaaca gcaccataat ggcacccgcg tagacgataa tttccagcgc
     6421 accggcgaag taagcgccta gtgagaagaa caccccggaa atcgccagca gcgaaataat
     6481 caggtacagc agtgcgtgta ccggattggt atgggtgatc actcgcaagg tcgcaagtat
     6541 ggctatcagg ccacagatat aaaaagcgaa ctccattgcc cctctcctta cggtaacagg
     6601 ctcttgacgt cgataggctt ggcttcgttc tctgcttcgc ccttatcttt gccgtcgatt
     6661 gccatacctg ccatccggta gaagttatac tccgggtatt tgcccggacc ggagatcagc
     6721 agatcctctt tctcgtaaac cagatcctgg cgcttgtatt cccccatttc gaaatccggg
     6781 gttaactgaa tcgccgtggt cggacaggct tcttcgcaca aaccacagaa aatgcagcgt
     6841 gagaagttga tgcggaaaaa ttccggatac cagcgaccgt ctttggtttc tgctttttgc
     6901 agcgagatac agccgaccgg gcaggctacc gcgcagaggt tacaggctac gcaacgctct
     6961 tcgccgtccg ggtcgcgggt cagaacgata cgaccacgat aacggggcgg cagatagacc
     7021 ggctcttccg ggtacattcg cgtttcgcgt ttggcgaacg cgtgcaggcc gatcatccag
     7081 atactacgaa cctgggtgcc gaaacctact aacaattctt ttaaggtcat ggtcttattg
     7141 ccccttattg cgcctgccag agaatgacag ccgccgttac cagcaagttg atcagcgtca
     7201 gcggcaggca gattttccag ccgaaggaca ttacctggtc ataacgcgga cgcggtaacg
     7261 acgcacgaat caaaatgaac atcatcataa agaacgcggt tttcagcgcg aaccagatga
     7321 atggcggtaa caacgggcct tgccagccac cgaagaacag cgtcaccatc aatgcagaga
     7381 tggtcacaat cccgatgtac tcacccacga agaacagacc gaacttcata ccggaatatt
     7441 caatgtggta accatccgcc agttcctgct cggcttccgg ctggtcaaac gggtgacggt
     7501 gacacaccgc cacgcccgcg atggcaaagg taataaaacc aaagaattgc gggatgacgt
     7561 tccacacatg cgcctggctg ttgacgatgt cggtcatgtt gaatgaaccg gcctgcgcca
     7621 ccacgcccat cagggaaagc ccgaggaaca cttcgtagct cagggtctgc gcagaagcac
     7681 gcatcgcacc gagcagcgag tatttgttgt tacttgacca gcccgcaaac agcaccgcgt
     7741 aaaccgccag accggccatc atcaggaaga acaaaatccc gatgttcagg tcggcaacca
     7801 cccaacccgg actgactggc acaatcgcaa aggccagcag cagcgaggta aaggcaatca
     7861 tcggtgccag ggtaaagatg acgcgatccg agaatttcgg gatccagtct tctttaaaga
     7921 acattttgat catgtccgca accagctgga gcgaaccacc ccagccaaca cggttaggtc
     7981 cgtaacggtt ctggaacaga cccagcaggc gacgttcgcc aaagctcatg aatgccccgc
     8041 aggtgacaac caccagcagg atcaccaccg ctttgaggat ggtcagcagg atctcaatca
     8101 gttccggtga tatccaactc attgttgtgc ctccttgaga tcctcaagat gcgcgccagc
     8161 cagcaccgga gcaatgccgg acatacccat cggcaagccc acctgccctg ccgtcagccc
     8221 ttcagcgatt tcaaccggca gagtgaccgt gttgccatcg taactaaagg agacgcgtgt
     8281 acctgcgttt acacccaact tcgcggcatc cgctgggttg agtttgatgt acggctgcgg
     8341 catacgacac tggaagaccg gagcacgctg tgacaattca tcgctgccaa acaggtggta
     8401 gtacggcgcg atacgccatt tcccgtcctg cggctggaag cgtgccggta cgctggtgaa
     8461 gtaatccaga ccattttcgc tggtttcaaa cagacgcaca cccggatcgc caaagcgcag
     8521 tttgccgccc acttcgtcct ggaatttgtt ccacgcctgc ggggagttcc agcccggagc
     8581 ccaggcaaac ggcacttgcg aacggtgcgc ggtcggctgg ttgttacctt ccatcgagaa
     8641 ggtgaacatg gtgtcaatat cctgcggctg acgcggctca tgaacgctga tattggcgcg
     8701 catggcggta cgaccgctgt aacggtgcgg ttcacgggcc agtttctgcc cacgaatacg
     8761 gaatgtcgca tctggcgcag catctttgat acccgccagt tccgggattt tcgccacaac
     8821 agcgtcaatt acatggtcga gctgcgtcca gtccacttca cggctcagca aggtgctgtg
     8881 cagcgagtgc agccagcgcc aactttccag catgacagtt ttgctgtcgt aataagccgg
     8941 atcgtaaacc tggaagaaac gttgggcgcg accttcatta ttaattaccg taccgtcgct
     9001 ttcagcaaag ctggcagcgg aaagcaccag atgggcgttt tccatgatcg ctgtgcgttg
     9061 atggtcaacc accatcacca gcggcgcttt agccagcgca gcattcacgc gggtagcaga
     9121 agcgtgacga tgcagatcgt tttccagcac caccaccgcg tcggcgcgtc cggtttccag
     9181 ttcggttaac gcttcttcaa gcgatccacc gcccataatg cccaacccca tgctgttgac
     9241 ggaacgggca atcatggtga taccgacgtc agcgccgcga cctttcaggg ctttcgcgac
     9301 gttagccgca gcctgaatca cctctaagct accggcgttc gtcccggaga taatcaacgg
     9361 tttcttcgca cccgccagtg cctgcacgat gacgtcgatt ttgctttgca gctcaggttc
     9421 aataccgtca accgctggcg cagagttatc cagcgcatgg gcgatggcaa aacctaaacg
     9481 cgcctgatct tcaaccggtg cgcggtaagt ccacgccgcg atatcatcca gacgggtgtc
     9541 atcaacgttg gtaacaaaca gcggatgctt agcacgttga ccgatattga ggattgccgc
     9601 aatctgccag tcagccactt tctgtgctgc cgccatttcg cgcgctttac ctttcaccgc
     9661 ctggcgcact gccagcgcga cgcgcgcgcc ggtctgggta acgtcttcgc ccagcaccag
     9721 taccgcatcg taagattcga tttcgcgcag agccggagta taaatgccgc cttcacgcag
     9781 cactttcagc gccagttgca gacgttcctg ctcaccgtga gcgataccgg tgtagaagtt
     9841 ttcttcgccc accagttcac gcagcgcaaa gttgctttcc acgctggcac gcggagaacc
     9901 aataccgatc actttcttcg actgacgcag aatatctgcc gcgccctgca ttgcctgttc
     9961 ggcgttgagg gtaatgaaat catcgccacg acgctgtact ggctgacgcg gacgatcttt
    10021 caggttgacg taaccgtaac cgaaacgacc acggtcgcag aggaagtagt ggtttaccgt
    10081 accgttgtaa cggttttcga tacgacgcag ttcgccataa cgctcgccgg ggctgatgtt
    10141 acagccgatg gaacattgct ggcagatgct cggcgcaaac tgcatatccc atttacggtt
    10201 gtaacgctcg gagtgcgttt tgtcggtaaa tacgccggtc gggcaaattt cgaccaggtt
    10261 accggaaaat tcgctttcca gcgtgccgtc ttccgggcga ccgaagtaga cgttgtcatg
    10321 cgcgccataa acgcccagat ctgtaccgtc agcgtaatct ttgtagtaac gcacgcagcg
    10381 gtagcaggcg atgcagcggt tcatttcgtg agagatgaac ggccccaaat cctgattacg
    10441 gtgggtacgt ttggtgaaac ggtagcgacg gaagctgtgt ccggtcatca cggtcatatc
    10501 ctgaagatgg cagttaccgc cttcttcaca taccggacag tcgtgcgggt ggttggtcat
    10561 taaccactcg accacacttt cacggaactg tttcgcttct tcgtcgtcaa tggaaataaa
    10621 ggtgccatcg gaagccggtg tcatacagga catcaccagg cgaccacgcg tgtcttccgc
    10681 gttttggtat tgcttcaccg cacactggcg gcaagcaccg acgcttccca gcgccggatg
    10741 ccagcaaaag taaggaatat caaggcccag agacagacaa gcttccagca ggttgtccgc
    10801 tccgttgacc tcgtattctt tgccgtctac atgaattgta gccattagca tgcttccagt
    10861 tttctcagtc agagactgag cgttaatcga aattcggtta ccagcgctct ttcagcaggt
    10921 tcggctgaat cccattaatc aaatgggtat tgctgaacgg ctgtttgatc cccgcctcaa
    10981 attcttcgcg gaaatatttg atggcgctct gtaacggctc cacagcacca ggtgcgtggg
    11041 cacagaaagt tttacccggg cctaagaatc gacacagttg ctcaagtgtt tcgatatcgc
    11101 ccggctgacc ttcgccacgc tccagcgcac gcagaatttt cacgctccac ggcagaccgt
    11161 cgcggcacgg cgtacaccag ccgcaggact cacgggcgaa aaactcttcc aggttacgca
    11221 ccagcgacac catgttgatt tcatggtcaa ccgccatcgc cagcgccgta cccagacggc
    11281 tgcccgcttt accgatactt tcgaattcca tcggcaggtc aaggtgcgct tcggtcagga
    11341 agtcggtgcc cgcgccgcct ggctgccagg ctttgaattt cagaccgtca cgcataccac
    11401 cggcgtaatc ttcgaggatc tcgcgtgcgg tggtgccgaa cggcagttcc cacagtcccg
    11461 gatttttcac ccgaccggag aagcccatca gcttggtgcc agcatcttta cttttcgaga
    11521 tgttctgata ccactccacg ccgttagcga ggatcgccgg aacgttacac agggtttcga
    11581 cgttgttgac acaggtcggt ttgccccata cgccggaggt tgccgggaag gggggtttcg
    11641 agcgtgggtt agcacgacgc ccttccaggg agttgattaa cgctgtttct tccccgcaga
    11701 tgtagcgccc tgccccggta tggacgaaca gttcgaagtc gaaacctgtt cccataatgt
    11761 ttttgccaag caatcccgct tcagtcgctt cggcaatggc gcggcgcaga tttactgccg
    11821 cttcaatata ttcgccacgc aggaagatgt agccacggta agctttcagc gcaaacgcgg
    11881 agatgagcat accttccacc agcaggtgcg gcagttgctc catcaacagg cggtctttat
    11941 aggtgcccgg ctccatttca tcggcgttac acagcaggta acggatgttc atggattcgt
    12001 ctttcggcat caggctccac ttcaagccag tcgaaaagcc cgcgccgccg cgccctttca
    12061 gaccagcgtc ttttacctga ttaacgattt cgtccggaga cagcccggtc agcgccttac
    12121 gcgccccttc gtaaccgttt ttgctgcggt attcgtccag ccacactggc tgtttgtcat
    12181 cgcgcagacg ccaggtcagc ggatgcgttt cgggagtacg gataatgttt ttcatttata
    12241 ccgctccagc agttcaggga tcgcttccgg ggtcagattc gcgtgagtgt cctcatcgat
    12301 catcatgttt ggccctttat cacagttccc caggcagcaa gttggcagca gcgtaaagcg
    12361 gccatcaaat gtcgtttgcc ctggtttgat gttcagcttt ttctcgagcg ccgcctgaat
    12421 accctgatac ccattgatat gacagaccac gctgtcgcaa taacggatca catggcggcc
    12481 aaccggctgg cggaagatct ggctgtagaa cgttgccacg ccttcgacgt cgcttgccgg
    12541 aatacccagc acatcggcga tcgcgtggat cgcagcatcc ggcacccagc cacgctgctt
    12601 ctgaacgatt ttcagcgctt caatggacgc cgcacgcggg tcttcgtagt ggtgcatctc
    12661 gtgctcgatc gcttcacgct ctgccgcact cagctcaaaa gcctcggttt gtggttgttg
    12721 attctcgtgc ataattagcg gtccacatct gacataacaa aatcgatact gcccagataa
    12781 acaatcaggt cagacaccag gctgccgcgg atcgccgccg gaatttgctg caaatgcgca
    12841 taactcggcg tgcggatacg agtgcgataa ctcatggtgc taccgtcgct ggtcaggtag
    12901 taactgttga tccctttggt cgcctcaatc atctggaagg attcattggc aggcatcacc
    12961 ggcccccacg acacttgcag gaagtgggtg atcagggttt cgatatgttg cagcgtgcgc
    13021 tctttcggcg gcggcgtggt cagcgggtga tccgctttga acgggccttc cggcatgttg
    13081 ttgaggcact gctcaagaat gcgcagactc tggcgcagct cttccacttt aagcattacg
    13141 cgggtgtagc agtcagaaac accaccacca accgggattt caaagtcgaa gttttcatag
    13201 ccagaataag gacgcgcctt acgcacgtcg aagtcgatcc cggtagcacg caggcccgcg
    13261 ccagtggtgc cccactccag cgcctctttc gcgccatagg cggcaacgcc ctgggaacga
    13321 cctttcagaa tggtattttg cagcgccgct ttctcgtaag acgccagacg tttcggcatc
    13381 cagtcgagga actcacgcag caggcgatcc cagccgcgcg gcaggtcgtg cgctacgccg
    13441 ccaatacgga accacgccgg gtgcatacgg aaacccgtga tcgcttccac cagatcgtaa
    13501 attttctgac gatcggtaaa ggcgaagaac acgggcgtca ttgcgccgac gtcctgaata
    13561 aaggtcgaga tatacagcag gtggctgttg atgcggaaca gttcggagag cattacgcga
    13621 atgacgttaa cgcgagccgg cacggtgatc ccggccagtt tctctaccgc cagcacgtaa
    13681 ggcatttcgt taacgcagcc gccgaggtat tcgatacggt cggtatacgg aatgtagctg
    13741 tgccaggact ggcgttcgcc cattttctcc gcaccacggt ggtggtagcc gatgtctggt
    13801 acgcagtcga caatctcttc gccgtcgagc tgcaaaacga tacggaaagc accgtgcgcc
    13861 gacgggtggt tcggaccgag gttgaggaac atgaagtcct cgttttcggt gccgcgcttc
    13921 atcccccact cttccggttt gaaggtcagg gcttccatct ccagatcctg tttggctttg
    13981 gtcagctcaa acggcgagaa ttcggtagcg cgcgccgggt aatctttacg cagcgggtga
    14041 cctttccagg tttgcggcat catgatgcgg cgcaggttcg ggtgaccgtc gaatgtaatg
    14101 ccaaacagat cccaggtttc acgctcatac cagttagcgt tcgggaacag tttggtgaag
    14161 gtcggtacgt gcaggtcgtt ttctgccagc gccaccttca gcatgatgtc gcggttacga
    14221 tcgatagaaa tcagatggta gaaaacggaa aaatccgcgg caggtaaccc ttcgcggtgc
    14281 gtgcgcagac gttcgtccat gccgtgtaag tcaaacagca tgacgtaagg tttcggcagt
    14341 ttctttaaga aatcgccaac ttccagtaat tgttcacgct tgatccacac aacgggaacc
    14401 ccggtgcgag tcgcctgaac agtaaaggca tccggcccaa aacggttgcg cagttcgcca
    14461 attaccggat catcaagatg atcgcgggtc tgccaggcgg gttcttgcgc ggttaagtcg
    14521 gtcatattgt tcaccattgc aaatggtccg tggtgactgt cggcgcagca agcttcgcga
    14581 aatagattta ggaataagcg aagaaaaatc cctttgccga caggcgcaaa ttaaatctcg
    14641 tcaggtgtac gcaggttggt tacggcaatg cgttcaccgc gcttgcgttc gcgctctgat
    14701 tgcatattgg cgcgataaac gccctgatcg ccaaccaccc aggagagcgg acgacgttct
    14761 ttgccgatag attcctgcaa cagcatcagt gcctgcatgt acgcttcagg acgcggcggg
    14821 cagcccggga tatacacatc aaccgggatg aatttatcga cgccctgcac aacggaataa
    14881 atatcgtaca taccaccaga gttggcacag gcacccattg agataaccca ttttggttcc
    14941 agcatctggt catacagacg ctgaataacc ggtgccattt tggtaaagca ggttcctgca
    15001 accaccatca ggtcagcctg acgcggcgaa gcacgcaata cttctgcgcc aaaacgcgcc
    15061 acgtcatgca ccgcggtaaa cgaagtcacc atctcaacgt aacagcagga aagaccgaag
    15121 ttatacggcc aaattgagtt tttacgaccc cagttaacca tgtcattgag cttgcccata
    15181 aacacgtttt tgttaacttc ttgctccaga gggtcggtta cgatctcctg cttttgcagg
    15241 gggtaacggt cgttctcacc gttgggatct atgcgggtga gcgtataatc catcttaatg
    15301 cctcgcggtt agcgttgacg attagcgata ctgttcgttt ccgggttcat acgctcgcgg
    15361 cgtgaacgcg cgggcgtcca gtccagcgcg ccaatacgca ccagataaac cagacctgcc
    15421 agtaacacaa aaataaaaat tgcagcttcc acaaagccta cccagccgct ttcgcggata
    15481 gaggttgacc atgcgaacag atacagcgct tcaacgtcga agataacgaa gaacatggcc
    15541 accagataaa acttggcgga caggcgtaag cgggcggagc cgaccgagtc gataccggat
    15601 tcaaacggca cgtttttcga cctcgcgcgt gcgcgaccgc ctaaaaacca accgcctacc
    15661 agcatcaggc aacataggcc aatggcaacg ataagaaaga tagcgaatgc ccagtgatga
    15721 gcgatgactt cagtggatgt tgacatactc attgcttact catcaaaagt agcgccagat
    15781 tcactgctct tcacggcaga tggacgccac atcgattcat ggggaggaat aaaaaaaacc
    15841 ttacaatcac tgtagaaatt cttttataca gctaattgat gtggtctttt actcctttct
    15901 ataacctttt gtcaacttta acaaaagttt cttcacatta gtttacataa tatcaacacc
    15961 attagcattt aatgcccttt caccccagat ccttgacgac tccaggataa ttagatgttg
    16021 ttgaatcgtg tccgttgtga agcaatggaa aaaatacggg tctattttga caggaatttg
    16081 tgtcgattcc tccccccaaa agagagtatt ttcttgatct gtgacacgct tttgtcattc
    16141 cataacaaaa acgcagcaac aaatttacgt attttttaac atcattgtag caggtgattt
    16201 ttttcaggcg attatttgtg cgtacggggc gtgaatctct ggtgattgaa aaatgaacag
    16261 ttttgtacgt tctgcacgat gtgaagaagg ttctttacta aaaaaaagcc gctggggttt
    16321 aaaacacccc cagcggctcg ttttttacac tattgtctca ggaattatct atcgtccgtc
    16381 gattactcga tatccctttc aatcaacaat gaatcatccc cttccggagc agatattggg
    16441 ctgtattgcc acggattatg gtagctctcc atcgcctgat agatcacctg ggccagctca
    16501 ttattactgg acgggtcata gcacagcaaa tattcggtat caggtagcgg cggtaagcca
    16561 tctactccac tcagcacacg caagtccgga ctcatcatct cgaccggcct tgccgtcacg
    16621 ccaagaccgg ctttcactgc cgcacgaacg gccggaagcg tcgaggcgac ataagccagt
    16681 cgccagggaa tatctgcttt attaagcgtc gccagcacca tatcgcgaaa cggacttgga
    16741 tcatccagca atacaagagg gatcggctca cctttttgca atacgtattc cgctgcgcag
    16801 taccagtgtg ttggcgaggt acgcaggttc aatgccttaa acgctgaagg acgatgggtg
    16861 gttaccatca aatccacttc ctgggattcc agcatttctg ccatataggc attacgttta
    16921 acgcggacat ccagcgctaa tttcggataa accgaactca cgcgatttaa caggaaaggt
    16981 aagatcgtat cggcagattc atctgaagca ccgatagtta acacgccctg aagattactg
    17041 aacattaatg atgagcaggc ctcatcatta aaacgcagga ttttcctggc gtaaccaaga
    17101 agttgaatgc catgttcagt taacagtttg ttgcgaccgt gacgagcgaa cagttctttc
    17161 ccaacgagtt gttccagacg ctgcatttgc tgacttacgg cggactgagt acgacacaca
    17221 gcggcagctg cggcagcaaa agtgttcaga tcggcaacag caacaaatgt tctcagcaga
    17281 tcgaggtcga ggttaattat cggacgattt gcacttatca tatattatca cttactggcg
    17341 gctcatactg agctggttaa tgctgtgcac acacaaacaa gcaattccat ttgtaatgtg
    17401 cctccctggc agtttcatcc ggaacccgga cgaaagtaaa aatgcatatg agttgcacta
    17461 aaaaagcgac tcacattgtt ccgttataat gcctgaagta gatcacagaa tatatcttca
    17521 gggatcgcat atctattaag ttactcactc ttttctattt atgacatgcg cgtgtttgta
    17581 taaatgtaaa tgtgagtcct tgttccactc tcgtgcagca tcgctggtca tacgcgaaca
    17641 cgtacaaaca gcaatggtga ggcggcatca agagcaggga tccgtcattt atgcgagcat
    17701 tttaccccaa aaacctttta tttataaggg tcattgcgaa ttatctgatg caaagttatg
    17761 ttatgttagg caaagt
//
LOCUS       DBFIFM010000088        17740 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.088, whole genome shotgun sequence.
ACCESSION   DBFIFM010000088 DBFIFM010000000
VERSION     DBFIFM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.088"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            23..748
                     /gene="yjjQ"
                     /locus_tag="N7C74_002539"
     CDS             23..748
                     /gene="yjjQ"
                     /locus_tag="N7C74_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2432386.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            706..1383
                     /gene="bglJ"
                     /locus_tag="N7C74_002540"
     CDS             706..1383
                     /gene="bglJ"
                     /locus_tag="N7C74_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HGU2432387.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(1421..2209)
                     /gene="fhuF"
                     /locus_tag="N7C74_002541"
     CDS             complement(1421..2209)
                     /gene="fhuF"
                     /locus_tag="N7C74_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HGU2432388.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDARLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIREYKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2350..2586
                     /gene="yjjZ"
                     /locus_tag="N7C74_002542"
     CDS             2350..2586
                     /gene="yjjZ"
                     /locus_tag="N7C74_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HGU2432389.1"
                     /translation="MLQRTLGSGWGVLLPGFLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            complement(2625..2711)
                     /locus_tag="N7C74_002543"
     tRNA            complement(2625..2711)
                     /locus_tag="N7C74_002543"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2675..2677),aa:Leu,seq:cag)
     gene            complement(2901..3932)
                     /gene="rsmC"
                     /locus_tag="N7C74_002544"
     CDS             complement(2901..3932)
                     /gene="rsmC"
                     /locus_tag="N7C74_002544"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HGU2432390.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFQHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4035..4448
                     /gene="holD"
                     /locus_tag="N7C74_002545"
     CDS             4035..4448
                     /gene="holD"
                     /locus_tag="N7C74_002545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HGU2432391.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4417..4863
                     /gene="rimI"
                     /locus_tag="N7C74_002546"
     CDS             4417..4863
                     /gene="rimI"
                     /locus_tag="N7C74_002546"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HGU2432392.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            4878..5555
                     /gene="yjjG"
                     /locus_tag="N7C74_002547"
     CDS             4878..5555
                     /gene="yjjG"
                     /locus_tag="N7C74_002547"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HGU2432393.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            5646..7235
                     /gene="prfC"
                     /locus_tag="N7C74_002548"
     CDS             5646..7235
                     /gene="prfC"
                     /locus_tag="N7C74_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HGU2432394.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            7628..8233
                     /gene="osmY"
                     /locus_tag="N7C74_002549"
     CDS             7628..8233
                     /gene="osmY"
                     /locus_tag="N7C74_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HGU2432395.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGIVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            8360..8521
                     /gene="ytjA"
                     /locus_tag="N7C74_002550"
     CDS             8360..8521
                     /gene="ytjA"
                     /locus_tag="N7C74_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HGU2432396.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            8643..9716
                     /gene="yjjU"
                     /locus_tag="N7C74_002551"
     CDS             8643..9716
                     /gene="yjjU"
                     /locus_tag="N7C74_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HGU2432397.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            9713..10495
                     /gene="yjjV"
                     /locus_tag="N7C74_002552"
     CDS             9713..10495
                     /gene="yjjV"
                     /locus_tag="N7C74_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU2432398.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGESGLDL
                     FGDDPQFERQQWFLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRVVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYAVFNVRG"
     gene            complement(10709..11572)
                     /gene="yjjW"
                     /locus_tag="N7C74_002553"
     CDS             complement(10709..11572)
                     /gene="yjjW"
                     /locus_tag="N7C74_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HGU2432399.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQYPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGVGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALRERG
                     VSRLIFPALYL"
     gene            complement(11544..13093)
                     /gene="yjjI"
                     /locus_tag="N7C74_002554"
                     /pseudo
     CDS             complement(11544..13093)
                     /gene="yjjI"
                     /locus_tag="N7C74_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
     gene            13351..14130
                     /gene="deoC"
                     /locus_tag="N7C74_002555"
     CDS             13351..14130
                     /gene="deoC"
                     /locus_tag="N7C74_002555"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HGU2432400.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            14257..15579
                     /gene="deoA"
                     /locus_tag="N7C74_002556"
     CDS             14257..15579
                     /gene="deoA"
                     /locus_tag="N7C74_002556"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HGU2432401.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            15631..16854
                     /gene="deoB"
                     /locus_tag="N7C74_002557"
     CDS             15631..16854
                     /gene="deoB"
                     /locus_tag="N7C74_002557"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGU2432402.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            16911..17630
                     /gene="deoD"
                     /locus_tag="N7C74_002558"
     CDS             16911..17630
                     /gene="deoD"
                     /locus_tag="N7C74_002558"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HGU2432403.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         4275 a         4521 c         4758 g         4186 t
ORIGIN      
        1 agacagtgga tgtggaggaa atatgttgcc aggatgctgc aaaaatggaa ttgttatcag
       61 taaaatacct gttatgcaag cagggttaaa agaggtcatg aggactcact tccctgaata
      121 tgaaataata tccagcgcct ctgcggagga ccttacctta ttacaattac gtcgttccgg
      181 attagtcatt gctgatttag ccggtgaaag tgaagatcca cgttctgttt gtgaacatta
      241 ttattcttta atctcacaat atcgggaaat tcactgggtt ttcatggtgt cacgctcttg
      301 gtattcccag gctgtagaac tgctcatgtg ccctacggcg acgttattgt ctgatgttga
      361 acccattgag aatctggtca agaccgtacg ttccggcaat acgcacgcag agcgtatcag
      421 cgccatgctg acctctccgg caatgactga aactcatgat tttagctatc gctccgtcat
      481 tctcactctt tcagagcgca aggtactgcg gctattaggt aaaggatggg gcatcaacca
      541 gatagcttca ttgcttaaga aaagtaataa aactatcagc gcccaaaaaa acagtgcgat
      601 gcgtcgactg gcaattcaca gcaacgctga aatgtatgca tggataaata gcgcgcaggg
      661 tgcaagagaa cttaacttgc cttctgttta tggagatgcc gcagaatgga acacagccga
      721 attaagaaga gaaatgtcgc actcatagaa aaatgcgtca tgagtagtat cggtattgag
      781 agtttattca gaaagtttgc gggcaaccct tataagctcc atacctatac cagtcaggag
      841 tcatttcagg atgccatgtc gcggatctcg tttgcggcgg tcattttttc tttttctgcc
      901 atgagaagtg agcgcagaga gggattatct tgcctgactg aactggcgat taagtttccg
      961 cgtacccggc gtttagttat tgcggatgat gatattgaag ctcggctgat tggttcattg
     1021 tcgccatcac cgctggacgg tgtattaagt aaagcgtcaa cgctggagat ttttcatcag
     1081 gaactctttt tgtcattaaa tggtgtacgt caggcgaccg accgactgaa caatcagtgg
     1141 tacattaacc aaagccggac gttaagcccg acggagagag aaatattgcg ctttatgtcg
     1201 cgtggctact caatgacaca aattgccgag cagcttaaac gcaatatcaa aacgatccgt
     1261 gcacataaat ttaatgtgat gtcgaaactg ggcgtcagtt ctgacgcagg gttgttggag
     1321 gccgcagata ttctgttatg tatgcggcat tgcgaaacaa gtaatgtgtt gcatccctat
     1381 taatccgcat gatgccgggt ttacttcccc ggcagtgctt tcatttcagc gtacaatcgc
     1441 cacattgctg tacatccggt aagcgataac gctggcagca agtgcggcgc accagcaggc
     1501 cgtcgcgtag caccacggta cgccagagtg gattatcttc accggtagta agcgttttct
     1561 caaaaaagag ggcatggcgc agcgattcaa ccgtcgcctc gccgagcagt tgcttcatct
     1621 cagtaagata ccagttgatc aaataaccgg tattactcca gataagtttg ccgttgatct
     1681 ctccggtcgc ttctagtgcc tgcacaaccg gaaccagcgc ctggctgatt aacgtttcca
     1741 ttcgctgctg cggcgaatgt ggtgttgcgt ttttatcttc acacacatcg acccagaaac
     1801 aggcgacgcg tccggtttcg tgaaactcag catggaaatg ttccggcgac acatctaatg
     1861 ctttttcctg cgtcagtagc gccagcatta atggtggcac catcaggccg atataccatt
     1921 gtgcccatag tgagatcagc ggtttgtact cgcggatcat cgttggttgg ttgcgataga
     1981 tatgatcgga atagaccgcc agtagagaac ttagcgcatt cggtgatgac cattgcgcca
     2041 gcgtcatggc gttaagcgga gcgggttcat ccaggcggat aaactccagc aaatgttcac
     2101 gatgttcggc gatcgtcgca cgtacggctt gtgcaagcct agcatcctgt ggctggagat
     2161 gcgttcgcca gatgatatct tcatagagcg gtgcggaacg ataggccata atcgggatag
     2221 taatctaaat gataatgatt gctaatccta gcgataggtt tacccgatag caagggattt
     2281 ctctggcttg caagtgataa aaattatcat atgatattgg ttatcattat caatgaaaga
     2341 gataaaacca tgttgcaacg tacgctgggc agtggctggg gagtgttgct gccgggattt
     2401 ctgattgcag ggctgatgta tgcggattta tcgccggatc agtggcggat tgtcattctg
     2461 atgggattag tattgacgcc ggtgatgctg tatcacaaac agttgcggca ttacattttg
     2521 ctaccatcgt gcctggcgct tattgctggc atcatgctga tgataatgaa tttgaatcag
     2581 ggatgaaaaa acagggaaga aacaagaaag gaagtaaaga taattggtgc gagggggggg
     2641 acttgaaccc ccacgtccgt aaggacacta acacctgaag ctagcgcgtc taccaattcc
     2701 gccaccttcg cataccatca attcttaaaa agaattgcta ccacggaggc gcattctagt
     2761 ggttttcagc ttttcgtcaa tagttaatta tcgcctgatg tgtaattgct ggaaaaatgt
     2821 ccatcaggag actaacgtgc aggttttgta agtcggataa ggcgttcacg ccgcatccga
     2881 cacggtattc ggcgagataa ttaacctttc ttcgcctggc gggtcataat ggcgcgatac
     2941 accttgaagc gccctgtttg cgcgatcact tcgtggaagc caaatgtctc atccagcacg
     3001 tccggataag gcaggaaggc gttcgctaca attcgcagct cgccgccgct attaagatga
     3061 cgcaccgcac cgcgaatcag cgtttgcgcc gcatccaggc tggtttgcat cccatcgtgg
     3121 aacggcgggt tggagatgat catatcaaaa cgacctttca cctcggaaaa gacgttgctg
     3181 gcaaagactt caccttcaac accgttggcc gcaagtgttg cgcggctggc ttctaccgct
     3241 ggcgcagaga catcgcacaa ggtgagacga attttcggcg aatggcgcgc aaaggcaacc
     3301 gaaagcacgc ccgcgccaca gccgacatcc agcactttac ctttcgtgtg cggggttaac
     3361 gtcgagagca gcaactggct accgacatcc agaccgtcgc ggctaaacac gccaggcagc
     3421 gttttgaccg tcaggccatc gacgctgtat tcgccccaga atttatcggc atcaaatacc
     3481 ggctgttttt ccagacgacc aaaatagagg ccacagcgac gggcgctgtc gactttattc
     3541 aacggcgcat aatcagccag catctgctcg gcgctgcgca cgccgctgcg gttctcgcca
     3601 acaacaaaaa tatccgtccc caccggaagc agagaaagta aattcatcaa ctggaactgg
     3661 gcttccggtt tgttcttcgg ccagtagtaa atcagcgtat cgcaatctgc gacatcatcc
     3721 gccgtggcga ccagactaaa acgggcgtta tcccccatct ggcggcttaa cacctgccaa
     3781 tgctggaatt gctgggtatg agcacggctg gccgcggtat ctaaacgcgc gggcaggtca
     3841 tcctgtaagt ctccggcaaa cagaatacgg ctttgttcga aatcatcact gtgacgcagc
     3901 aagacttcac ttgccggggt aaatgcagac atggaatgct cctcaattga tactggcggc
     3961 gattatagcc atatgttggc gcggtatcga cgaatttgct atatttgcgc ccctgacaac
     4021 aggagcgatt cgctatgaca tcccgacgag actggcagtt acagcaactg ggcattaccc
     4081 agtggtcgct gcgtcgccct ggcgcgttgc agggggagat tgccattgcg atcccggcac
     4141 acgtccgtct ggtgatggtg gcaaacgatc ttcccgccct gactgatcct ttagtgagcg
     4201 atgttctgcg cgcattaacc gtcagccccg atcaagtgct gcaactgacg ccagaaaaaa
     4261 tcgcgatgct gccgcaaggc agtcgctgca acagctggcg gttgggtact gacgaaccgc
     4321 tatcactgga aggcgctcag gtggcatcac cggcgctcac cgaattacgg gcaaacccaa
     4381 cggcacgcgc cgcgttatgg caacaaattt gcacatatga acacgatttc ttccctcgaa
     4441 acgactgatt taccagcggc ttaccacatt gaacaacgcg cccacgcctt tccgtggagt
     4501 gaaaaaacct ttgccagcaa ccagggcgag cgttatctca actttcagtt aacgcaaaac
     4561 ggcaaaatgg cggcgtttgc gattacgcaa gtggtgctgg atgaagctac attgttcaat
     4621 attgcggtcg atcctgacta tcagcgtcag ggattgggaa gggcgctgct ggaacatctg
     4681 atcgacgaac tggaaaaacg cggcgtggcg acactatggc tggaagtccg tgcctcaaat
     4741 gctgccgcca ttgccctgta cgaaagttta ggctttaacg aggcgacgat tcgccgcaat
     4801 tactacccca ccacggacgg tcgcgaagac gccatcatca tggcgttgcc aatcagtatg
     4861 taagacaagg tggaataatg aagtgggact ggattttctt tgatgccgat gaaacgctgt
     4921 ttacctttga ctcattcacc ggcctgcagc ggatgtttct tgattacagc gtcaccttta
     4981 ccgctgaaga ttttcaggac tatcaggccg ttaacaagcc actgtgggtg gattatcaaa
     5041 acggcgcgat cacttcatta cagcttcagc acgggcggtt tgagagctgg gccgaacggc
     5101 tgaacgtcga gccaggtaaa ctcaacgaag cctttattaa tgcgatggcg gaaatctgca
     5161 cgccgctgcc gggcgcggtt tctctgctta acgccattcg tggcaacgcc aaaatcggca
     5221 tcatcaccaa cggctttagt gccttgcaac aggtgcgtct ggaacgcacg ggcctgcgtg
     5281 attacttcga tttgctggtg atttccgaag aagttggcgt tgccaaaccg aataagaaaa
     5341 ttttcgatta tgcgctggaa caggcgggca atcctgaccg ttcacgcgtg ctgatggttg
     5401 gcgacactgc cgagtccgat attctcggtg gcatcaacgc cgggcttgcg acctgctggc
     5461 tgaatgcaca ccatcgcgag caaccagaag gcatcgcgcc cacctggacc gtttcttcgt
     5521 tgcacgaact ggagcagctc ctgtgtaaac actgattgcc tcccccccgt tgatgggtaa
     5581 aatagccgca atttttcgtt ttcaacaagc gcggcgcgat gccgcttact caagaagaaa
     5641 gaattatgac gttgtctcct tatttgcaag aggtggcgaa gcgccgcact tttgccatta
     5701 tttctcaccc ggacgccggt aagactacca tcaccgagaa ggtgctgctg ttcggacagg
     5761 ccattcagac cgccggtaca gtaaaaggcc gtggttccaa ccagcacgct aagtcggact
     5821 ggatggagat ggaaaagcag cgtgggatct ccattactac gtctgtgatg cagtttccgt
     5881 atcacgattg cctggttaac ctgctcgaca ccccggggca cgaagacttc tcggaagata
     5941 cctatcgtac cctgacggcg gtggactgct gcctgatggt tatcgacgcc gcaaaaggtg
     6001 ttgaagatcg tacccgtaag ctgatggaag ttacccgtct gcgcgacacg ccgatcctca
     6061 cctttatgaa caaacttgac cgtgatatcc gcgacccgat ggaattgctc gatgaagttg
     6121 agaacgagct gaaaattggc tgcgcaccga tcacctggcc aattggctgc ggcaagctgt
     6181 ttaaaggcgt ttaccacctt tataaagatg aaacctatct ctatcagagc ggtaaagggc
     6241 acaccattca ggaagtccgc attgttaaag ggctgaataa cccggatctc gatgctgcgg
     6301 ttggtgaaga tctggcacag cagctgcgtg acgaactgga actggtgaaa ggcgcgtcta
     6361 acgagttcga caaagagctg ttccttgcgg gcgaaatcac tccggtattc ttcggtactg
     6421 cgctgggtaa cttcggcgtt gatcatatgc tggatggcct ggtggagtgg gcgcctgcgc
     6481 cgatgccgcg tcagaccgat acccgtaccg tagaagcgag cgaagataaa tttaccggct
     6541 tcgtatttaa aattcaggcc aacatggacc cgaaacaccg cgaccgcgtg gcgtttatgc
     6601 gtgtggtgtc cggtaaatat gaaaaaggca tgaagctgcg ccaggtgcgc actgcgaaag
     6661 atgtggtgat ctccgacgcg ctgaccttta tggcgggtga ccgttcgcac gttgaagaag
     6721 cgtatccggg cgatatcctc ggcctgcaca accacggcac cattcagatc ggcgacacct
     6781 ttacccaggg tgagatgatg aagttcaccg gtattccgaa cttcgcacca gaactgttcc
     6841 gtcgtatccg cctgaaagat ccgctgaagc aaaaacagct gctcaaaggg ctggtacagc
     6901 tttccgaaga gggcgcggtg caggtgttcc gtccaatctc caacaacgat ctgatcgttg
     6961 gtgcggttgg tgtgctgcag tttgatgtgg tggtagcgcg cctgaagagc gaatacaacg
     7021 ttgaagcagt gtatgagtca gtcaacgttg ccactgcccg ctgggtagaa tgtgcagacg
     7081 cgaagaaatt cgaagagttc aagcgtaaga acgaaagcca actggcgctt gatggcggcg
     7141 ataacctcgc ttacatcgct accagcatgg tcaacctgcg cctggcacag gaacgttatc
     7201 cggacgttca gttccaccag acccgcgagc attaattctt gcctccaggg cgcggtagcc
     7261 gctgcgtcct gtcaatttcc cttccttatt agccgcttac ggaatgttct taaaacattc
     7321 acttttgctt atgttttcgc tgatatcccg agcggtttca aaattgtgat ctatatttaa
     7381 caaagtgatg acatttctga cggcgttaaa taccgttcaa tgcgtagata tcagtatcta
     7441 aagccgtcga ttgtcattct accgatatta ataactgatt cagaggctgt aatggtcgtt
     7501 attcatcact catcgctttt gtgatggcga ccattgactt ctgtagaggg tgaagtctct
     7561 ccctattcag caatgcaacc tcgtgttgcc aggctcaaat tacgagcaaa catacaggaa
     7621 taaatcgatg actatgacaa gactgaagat ttcgaaaact ctgctggctg taatgttgac
     7681 ctctgccgtc gcgaccggct ctgcctacgc ggaaaacaac gcgcagacta ccaatgaaag
     7741 cgcagggcaa aaagtcgata gctctatgaa taaagtcggt aatttcatgg atgacagcgc
     7801 catcaccgcg aaagtgaagg cggcgctggt ggatcatgac aacatcaaga gcaccgatat
     7861 ctctgtcaaa accgatcaaa aagtcgtgac cctgagcggt ttcgttgaaa gccaggccca
     7921 ggccgaagag gcagtgaaag tggcgaaagg tgttgaaggg gtgacctctg tcagcgacaa
     7981 actgcacgtt cgcgacgcta aagaaggctc tgtgaagggc tacgcgggtg acaccgccac
     8041 caccagtgaa atcaaagcca aattgctggc ggacgatatc gtcccttccc gtcatgtgaa
     8101 agttgaaacc accgatggcg tggttcagct ctctggtatc gtagattctc aggcacaaag
     8161 tgaccgtgct gaaagtatcg ccaaagcggt agatggtgtg aaaagcgtta aaaatgatct
     8221 gaaaactaag taattcgtcg taattcgtcc tcccgaactt tgtcgggagg cgcgatgtgc
     8281 accacactaa aaatatcgca ttgagtgatt ttcacgctca tatcaagcgg tgacattaac
     8341 tatggtaaag gagacgctta tgtttcgttg gggcatcata tttctggtta tcgcgttaat
     8401 cgccgccgca cttgggtttg gtggtctggc cggtaccgct gcaggcgcag ctaaaattgt
     8461 ctttgtcgtc gggattattc tgttcctggt gagtttgttc atgggccgaa aacgacccta
     8521 gatttcagac cattaagaaa attttatcca aagccagtcc agcggactgg cttttgcggt
     8581 tttagcgaat aataaattgc gttactttgt cattctttca caacaaaaac aggaaggcag
     8641 aggtggggca gcgaatacct gtaacgcttg gtaatattgc gccgttgtcg ctaaggccgt
     8701 tccagcctgg acgaatagct ctggtgtgcg aaggcggcgg acagcgtgga attttcacgg
     8761 ctggcgtgct ggatgagttt atgcgcgcgc agtttaatcc tttcgatctt tatctcggca
     8821 catctgccgg ggcgcagaac ctctcggcgt atatctgcaa tcagcccggt tacgcgcgca
     8881 aagtcatcat gcgctatacc acaaaacgcg aatttttcga tccattgcgc tttgtccgtg
     8941 gaggaaatct tatcgatctc gactggctgg tggaggccac cgcaagccag atgccgttgc
     9001 aaatggatac cgccgcgcgg ttgtttgaca gcggcaaatc gttttatatg tgcgcctgtc
     9061 gtcaggatga ctacgcgccg aattactttt taccaaccaa acaaaactgg ctggatgtga
     9121 ttcgcgcctc cagtgcgata cctggctttt atcgtagcgg agtgtcgctg gaaggcatta
     9181 actacctgga tggcgggatc agtgatgcga ttccggttaa agaggcggca aggcagggcg
     9241 ctaaaacgtt ggtcgtcatc cgcactgtgc cgtcacaaat gtactacacg ccgcagtggt
     9301 tcaaacgcat ggaacgctgg ctgggtgaca gtagcctgca gccgctggtc aatctggtgc
     9361 agcatcatga aaccagctat cgtgacattc agcaatttat tgagaaacca ccgggcaagc
     9421 tgcggatatt cgaaatttat ccgccgaagc cattacatag tatcgcgctt ggcagtcgga
     9481 ttccggcgct gcgtgaagac tataaacttg ggcgtttatg cggtcgttat ttcctcgcca
     9541 cggttggcaa gctattaact gaaaaagcgc cgcttacccg ccatctggtg ccagtggtga
     9601 cgccggaatc gattgtcatt ccgcctgcgc cagtcgccaa cgatacgctg gttgccgaag
     9661 tgagcgacgc tccgcaggcg aacgacccga catttaacaa tgaggatctg gcttgatttg
     9721 ccgttttatc gacacccact gccattttga tttcccgcct tttattggcg atgaagaggc
     9781 cagcctgcaa cgcgcggcac aagcgggcgt aggcaagatc attgttccgg caaccgaggc
     9841 ggaaaatttt gcccgcgtgc aggcgttagc ggaaaattat caaccgctgt atgccgcatt
     9901 gggcttgcat cctggtatgt tggaaaaaca tagcgatgtg tctcttgatc agctacagca
     9961 ggcgctggaa aggcgtccgg cgaaggtggt ggcggtgggg gagagcggtc tggatctctt
    10021 cggcgacgat ccgcaatttg agaggcagca gtggtttctc gacgaacaac tgaaactggc
    10081 gaaacgctac gatctgccgg tgatcctgca ttcacggcgc acacacgaca aactggcgat
    10141 gcatcttaaa cgccacgatt tatcgcgcac tggcgtggtt cacggttttt ccggcagcct
    10201 gcaacaggcc gagcggtttg tacagctggg ctacaaaatc ggcgtaggcg gtactatcac
    10261 ctatccacgc gccagtaaaa cccgcgttgt catcgcaaaa ttaccgctgg catcgttgtt
    10321 gctggaaacc gacgcgccgg atatgccgct caacggtttt caggggcagc ctaaccgccc
    10381 ggaacaggct gcgcgggtgt ttgacgtgct ttgcgagttg cgcccggaac cagaagatga
    10441 gattgccgaa gtgttgctta ataacacgta tgcggtgttt aacgttcgtg ggtagtttgc
    10501 ccttcgtagg cagcatccgg cagtcggcgc ataatgcctg atgcgacgct taacgcgtct
    10561 tatcatgcct acaggtttgt gccgaaccgt aggccggata aggcgttcac gccgcatccg
    10621 gcagttggcg cacaatgcct gatgcgacgc ttgacgcgtc ttatcaggcc tacaagtctg
    10681 tgccgaaccg taggtcgtat ccggcatgtc acaaatacag cgccggaaat attaaccggc
    10741 tcaccccgcg ctcccgtaac gcatcagcca acggctcaac gtcttccggc gtggcgctcg
    10801 cccagctttg cgcctcgcca tacacgccgt gggcatgaaa cgcgttcagg cgtaccggaa
    10861 catcgccgac tcccttgata aacgccgcca gttcttcgat gtgttgcaaa taatccactt
    10921 ggccagggat taccagcaaa cgcagttccg ccagcttacc gcgctctgcc agcaaacaga
    10981 tgctgcgctt aatctgctga ttatcgcgtc cggttaattg ctgatgacat tcgctccccc
    11041 acgctttgag atcgagcatt gcgccgtcgc acaccgggag caatttttcc cagccggttt
    11101 cgctcaacat accgttactg tccaccaggc aggtgaggtg gcgcagttgc ggatcgtttt
    11161 tgatagcagt aaacagcgcc actacaaacg gcagttgggt cgtggcttcg ccgccactca
    11221 ccgttatccc ttcgataaac agcactgctt tgcggacatg gctaagcacc tcatccacgc
    11281 tcatggattg cgccattggc gtggcatgtt gcggacacct cttcaggcag gtatcacatt
    11341 gctcgcagac cacagcgttc cacaccactt tgccgtcaac aatctgcaac gcctgatgcg
    11401 gatactgtgg cacgcactcc ccacagtcat tgcaacgtcc catcgtccac ggattgtgac
    11461 agtttttgca gcgcagattg cagccctgca aaaacagagc cagacgactg cctggcccgt
    11521 caacgcagga gaaggggata atcttactga ctaaagcgca tctgctgttc atggcttatc
    11581 acgcgcggct ggcgttccag aatacgagtg ttgcgtgcgg cttcttcgcc cagccaggtg
    11641 gtgttggtgc gtgaaccttc ggcgcgatat ttttctaaat ccgacaaacg caccatataa
    11701 ccggtaacgc gaaccagatc gttaccgctg acattggcgg taaattcacg cattccggct
    11761 ttaaaggcac cgaggcaaag ctgtaccaac gcctgcgggt tacgtttgat ggtttcgtcg
    11821 agcgtcagaa tgtcgctgat gccagaataa taataagcat gatgcgggcg acagtttgca
    11881 gatgggtgat cggatctggc tcatcgccat acggcaaacg cgcgcccggc gtggtgccga
    11941 tatcggaact gatccccgac tgtgcgtgta acatggcgcg tttttgccag ccatatttca
    12001 cgggggtatt ggcgacaaac tccgccagct gtgcactgat gcgataacct acttcatttg
    12061 cggcggcttc tttaccgtag cgcgcggcaa tcccttcttt ttcacacagc aagttaaccg
    12121 cttccgccag cccatacatg ccaaacattg gcacaaaacg ttcagggtta atcagccctt
    12181 ctttcaccag gaagctattc tcaaagaagt gtgattgttg atagaggaat tcacaccgcg
    12241 catcgatgat ggcgatctgc tgctggcagt agtgcggtag agtgcgcgta aagaagtcat
    12301 ccagcgattc gctgcgctcg gcaatggctt tcaggttaag gcgtaccagc gtgctgccac
    12361 cacccgccag cggcagtgag ttgtaacagc tcacaatccc gtagccccct tttgtgaaaa
    12421 ttttatcatg caccggaccg ttggcgatgt gcggtttgct acattcacag atgttcttcg
    12481 ccacttccag cagcaggtca tcaggggtga tttcaggatc gtagataaag gtcaggttcg
    12541 gtgaaacctg cttcaaatct gcgtctgcac gtaagatcgc gcgggtaatg ggcgaatcag
    12601 acgggccgat attggcgtgc ataaaggcgt ctggcagggt tctgtcgagg taacgccaga
    12661 aacgttttat tcgaacatcg atctcgtctt gtgttagaat tataacatac ggttgcaaca
    12721 acgcatccag ttgccccagg tagaccggca tcgatgtgac cgacggtacg tggtggtaaa
    12781 gaatggtcag cagagagagt gcgtcatcaa gatctttcgc gccttccagc tccagccatt
    12841 cggaaccgtt cgccagaaaa cgggcgtaat cgggtaagac atagcgcggt ttgtacggcg
    12901 catgaccttc aaacatatcg cagattacac cttcatccag cgcgcggcgg gcttcggcag
    12961 gaagctgtgg gtaaggcaga ttgttttctg cttccagtgc cagaaaatgg cgcttctgct
    13021 ccgggctaag cactgggctg gtgacaattt gctggcaacg ttgttgcagt gcattttcat
    13081 gagaagtggg catcttcttt tccttttatg ccgaaggtga tgcgccattg taagaagttt
    13141 cgtgatgttc actttgatcc tgatgcgttt gccaccactg acgcattcat ttgaaagtga
    13201 attatttgaa ccagatcgca ttatagtgat gcaaacttgt aagtagattt ccttaattgt
    13261 gatgtatatc gaagtgtgtt gcggagtaga tgttagaata ctaacaaact cgcaaggtga
    13321 attttattgg cgacaagcca ggagaatgaa atgactgatc tgaaagcaag cagcctgcgt
    13381 gcactgaaat tgatggacct gaccaccctg aatgacgacg acaccgacga gaaagtaatt
    13441 gctctgtgtc atcaggccaa aactccggtc ggcaataccg ccgctatctg tatctatcct
    13501 cgctttatcc cgattgctcg caaaacactg aaagagcagg gcaccccgga aatccgtatt
    13561 gctacggtaa ccaacttccc acacggtaac gacgacatcg aaatcgcgct ggcagaaacc
    13621 cgtgcggcaa tcgcctacgg tgctgatgaa gttgacgttg tgttcccgta ccgcgcgctg
    13681 atggcgggta acgagcaggt tggttttgac ctggtgaaag cctgtaaaga ggcttgcgcg
    13741 gcagcgaatg tactgctgaa agtgatcatc gaaaccggcg aactgaaaga cgaagcgctg
    13801 atccgtaaag cgtctgaaat ctccatcaaa gcgggtgcgg acttcatcaa aacctctacc
    13861 ggtaaagtgg ctgtgaacgc gacgccggaa agcgcgcgca tcatgatgga agtgatccgt
    13921 gatatgggcg tagaaaaaac cgttggtttc aaaccggcgg gcggcgtgcg tactgcggaa
    13981 gatgcgcaga aatatctcgc catcgcagat gaactgttcg gtgctgactg ggctgatgcg
    14041 cgtcactacc gctttggtgc ttccagcctg ctggcaagcc tgctgaaagc gctgggtcac
    14101 ggcgacggta agagcgccag cagctactaa gtaagatgct ttacgcctga tgcgctgcgc
    14161 ttatcaggcc tacgagacgt atctacccgt aggccggata aggcgtagac gcatccggca
    14221 aaagccacct catactcttt tcctcgggag gttaccttgt ttctcgcaca agaaattatt
    14281 cgtaaaaaac gtgatggtca tgcgctgagc gatgaagaaa ttcgtttctt tatcaacggt
    14341 attcgcgaca acactatctc cgaagggcag attgccgccc tcgcgatgac cattttcttc
    14401 cacgatatga caatgcctga gcgtgtctcg ctgaccatgg cgatgcgaga ttcaggaacc
    14461 gttctcgact ggaaaagcct gcatctgaat ggcccgattg ttgataaaca ctccaccggt
    14521 ggcgtcggcg atgtgacttc gctgatgttg gggccgatgg tcgcagcctg cggcggctat
    14581 attccgatga tctctggtcg cggcctcggt catactggcg gtacgctcga caaactggaa
    14641 tccatccctg gcttcgacat tttcccggat gacaaccgtt tccgcgaaat tattaaagac
    14701 gtcggcgtgg cgattatcgg tcagaccagc tcactggctc cggcggataa acgtttctac
    14761 gcgacccgtg atattaccgc aaccgtggac tccatcccgc tgatcactgc ctcgatcctg
    14821 gcgaagaaac tggcggaagg tctggacgcg ctggtgatgg acgtgaaagt gggtagcggc
    14881 gcgtttatgc cgacctacga actctctgaa gcccttgccg aagcgattgt tggcgtggct
    14941 aacggcgcag gcgtgcgcac caccgcactg ctcaccgaca tgaatcaggt gctggcctcc
    15001 agtgccggta acgcggttga agtacgtgaa gcggtgcagt tcctgacggg tgaatatcgt
    15061 aacccgcgtc tgtttgatgt cacgatggcg ctgtgtgtgg agatgcttat ctccggcaaa
    15121 ctggcgaaag atgacgccga agcgcgcgcg aaattgcagg cggtgctgga caacggtaaa
    15181 gcggcagaag tctttggtcg tatggtagcg gcacaaaaag gcccgaccga cttcgttgag
    15241 aactacgcga agtatctgcc gacagcgatg ctgacgaaag cagtctatgc tgataccgaa
    15301 ggttttgtca gtgaaatgga tacccgcgcg ctggggatgg cagtggttgc aatgggcggc
    15361 ggacgccgtc aggcatctga caccatcgat tacagcgtcg gctttactga tatggcacgt
    15421 ctgggcgacc aggtagacgg ccagcgtccg ctggcggtta tccacgcgaa agacgaaaac
    15481 agctggcagg aagcggcgaa agcggtgaaa gcggcaatta aacttgccga taaagcaccg
    15541 gaaagcacac caactgtcta tcgccgtatc agcgaataac ggtatactga tctgatcatt
    15601 taaatttgaa gcactgagta cggagaacat atgaaacgtg catttattat ggtgctggac
    15661 tcattcggca tcggcgctac agaagatgca gaacgctttg gtgacgtcgg ggctgacacc
    15721 ctgggtcata tcgcagaagc ttgtgccaaa ggcgaagctg ataacggtcg taaaggcccg
    15781 ctcaatctgc caaatctgac ccgtctgggg ctggcgaaag cacacgaagg ttctaccggt
    15841 ttcattccgg cgggaatgga cggcaacgct gaagttatcg gcgcgtacgc atgggcgcac
    15901 gaaatgtcat ccggtaaaga taccccgtct ggtcactggg aaattgccgg tgtcccggtt
    15961 ctgtttgagt ggggatattt ctccgatcac gaaaacagct tcccgcaaga gctgctggat
    16021 aaactggtcg aacgcgctaa tctgccgggt tacctcggta actgccactc ttccggtacg
    16081 gtcattctgg atcaactggg cgaagagcac atgaaaaccg gcaagccgat tttctatacc
    16141 tccgctgact ccgtgttcca gattgcctgc catgaagaaa ctttcggtct ggataaactc
    16201 tacgaactgt gcgaaatcgc ccgtgaagag ctgaccaacg gcggctacaa tatcggtcgt
    16261 gttatcgctc gtccgtttat cggcgacaaa gccggtaact tccagcgtac cggtaaccgt
    16321 cacgacctgg ctgttgagcc gccagcaccg accgtgctgc agaaactggt tgatgaaaaa
    16381 cacggccagg tggtttcggt cggtaaaatt gcagacatct acgccaactg cggtatcacc
    16441 aaaaaagtga aagcgactgg cctggacgcg ctgtttgacg ccaccatcaa agagatgaaa
    16501 gaagcgggtg ataacaccat cgtcttcacc aacttcgttg acttcgactc ttcctggggc
    16561 caccgtcgcg acgtcgccgg ttatgctgcg ggtctggaac tgttcgaccg ccgtctgccg
    16621 gagctgatgt ctctgctgcg cgatgacgac atcctgatcc tcaccgctga ccacggctgt
    16681 gatccgacct ggaccggtac tgaccacacg cgtgaacaca ttccggtact ggtttacggc
    16741 ccgaaagtaa aaccgggctc actgggtcac cgtgaaacct tcgcggatat cggccagact
    16801 ctggcaaaat attttggtac ttctgatatg gaatatggca aagccatgtt ctgatggatt
    16861 tgggcggagc gttgactccg cctttgttat gtcacaaaaa ggataaaaca atggctaccc
    16921 cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca ggcgacccgc
    16981 tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg aacaacgttc
    17041 gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta atgggtcacg
    17101 gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat ttcggcgtga
    17161 agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa ctgcgcgacg
    17221 tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt tttaaagacc
    17281 atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat gcagctaaag
    17341 cactgggcat tgatgctcgc gttggtaacc tgttctccgc tgacctgttc tactctccgg
    17401 acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa atggaagcgg
    17461 ctggtatcta cggcgtggct gcagagtttg gcgcgaaagc cctgaccatc tgcaccgtgt
    17521 ctgaccacat ccgcactcac gagcaaacca ctgccgctga gcgtcagacc accttcaacg
    17581 acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagagtaa ttgtgtttcg
    17641 ctgaaaggcg attgtcgtgt taagccggag tgggggactg ctccggcttt ttagtatcta
    17701 ttcatcaata gcaactgatt tatagattta ctattatttt
//
LOCUS       DBFIFM010000089        17686 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.089, whole genome shotgun sequence.
ACCESSION   DBFIFM010000089 DBFIFM010000000
VERSION     DBFIFM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.089"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            91..633
                     /gene="ecpR"
                     /locus_tag="N7C74_002559"
                     /gene_synonym="matA"
     CDS             91..633
                     /gene="ecpR"
                     /locus_tag="N7C74_002559"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HGU2432404.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            708..1295
                     /gene="ecpA"
                     /locus_tag="N7C74_002560"
                     /gene_synonym="matB"
     CDS             708..1295
                     /gene="ecpA"
                     /locus_tag="N7C74_002560"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HGU2432405.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1353..2021
                     /gene="ecpB"
                     /locus_tag="N7C74_002561"
                     /gene_synonym="yagY"
     CDS             1353..2021
                     /gene="ecpB"
                     /locus_tag="N7C74_002561"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HGU2432406.1"
                     /translation="MKKHLLLLALLLSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGTLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2047..4572
                     /gene="ecpC"
                     /locus_tag="N7C74_002562"
                     /gene_synonym="yagX"
     CDS             2047..4572
                     /gene="ecpC"
                     /locus_tag="N7C74_002562"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HGU2432407.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGIYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGTLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4562..6205
                     /gene="ecpD"
                     /locus_tag="N7C74_002563"
     CDS             4562..6205
                     /gene="ecpD"
                     /locus_tag="N7C74_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HGU2432408.1"
                     /translation="MRVNLLIAMIIFALIWPVTALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDTSGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNQGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6174..6884
                     /gene="ecpE"
                     /locus_tag="N7C74_002564"
     CDS             6174..6884
                     /gene="ecpE"
                     /locus_tag="N7C74_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HGU2432409.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPQDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPEDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7197..7526
                     /gene="ykgJ"
                     /locus_tag="N7C74_002565"
     CDS             7197..7526
                     /gene="ykgJ"
                     /locus_tag="N7C74_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HGU2432410.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(7774..8388)
                     /gene="yagU"
                     /locus_tag="N7C74_002566"
     CDS             complement(7774..8388)
                     /gene="yagU"
                     /locus_tag="N7C74_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HGU2432411.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            8806..9495
                     /gene="paoA"
                     /locus_tag="N7C74_002567"
     CDS             8806..9495
                     /gene="paoA"
                     /locus_tag="N7C74_002567"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HGU2432412.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAAAAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSPPERTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            9492..10448
                     /gene="paoB"
                     /locus_tag="N7C74_002568"
     CDS             9492..10448
                     /gene="paoB"
                     /locus_tag="N7C74_002568"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HGU2432413.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLVAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            10445..12643
                     /gene="paoC"
                     /locus_tag="N7C74_002569"
     CDS             10445..12643
                     /gene="paoC"
                     /locus_tag="N7C74_002569"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HGU2432414.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     SPNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLPVITASNAGALSKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVSQPPEDTPDK
                     NVGDFDGAFSSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPSIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQIDPADP
                     TRRFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEPNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACVKLREMIASAVGFDPEQSQFADGKITNGTRSAILHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            12653..13609
                     /gene="paoD"
                     /locus_tag="N7C74_002570"
     CDS             12653..13609
                     /gene="paoD"
                     /locus_tag="N7C74_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HGU2432415.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGCDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARNAHTLALSVLAEVASVRLHQEEDSCLPPSS"
     gene            complement(13788..14915)
                     /locus_tag="N7C74_002571"
     CDS             complement(13788..14915)
                     /locus_tag="N7C74_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2432416.1"
                     /translation="MTLCVFVLIASEFMPVSLLTPIARDLGVTEGLAGRGIAISGALA
                     VLTSLTLSTLAGKMNRKFLLLGMTVLMAVSGLIIALATSYLMYMVGRAMIGVAIGGFW
                     SMSAATAIRLVPQHQVTRALAIFNAGNALATVVAAPLGSYLGATVGWRGAFLCLVPMA
                     VVAFIWQCISLPSMDANKSHTSCGAVFRLFSRRMVVVGMIACGLFFMGQFALFTYVRP
                     FLESVTRVNSLGLSLILLIIGVAGFIGTLVVSIFLNRKFYPTLMAIPGLMAVIAIGLM
                     LTGHHVWMVSLLLGLWGMLATAAPTGWWTWIARTLPDNAEAGGGLMVAVIQLSIALGS
                     TAGGIVFDRLGWQSTFAMSSVLLLCAGVLTFVTARQKAGAL"
     gene            complement(15057..16115)
                     /locus_tag="N7C74_002572"
     CDS             complement(15057..16115)
                     /locus_tag="N7C74_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU2432417.1"
                     /translation="MSYADSTNPNAPVSMTDKWDKTFAESQKVDHRKVSFPNRYGITL
                     VGDLYLPKDRGDRKLAAIAVSGPFGAVKEQSSGLYAQTLAERGFVTLAFDPSYTGESG
                     GYPRNVASPDINTEDFSAAVDFLGLQKEVDRNRIGLLGICGWGGMALNDAAMDTRVKA
                     VATSVMYDMSRAMGHGVGDGEDRYSTADRRAVLQYLNEQRWKDAQSGTFAHGGHDINV
                     DSNGKVSAGERILPETLPANPHPVLKEFFDYYRMPRGFHERSVNSTGAWTATMPLSFM
                     NMPLLSYASEITIPTLIVTGEKAHSRYFAEDAYKAVGSKDKELVVVPGANHVDLYDNV
                     AGKIPFAKFEQFFQTKLK"
     gene            16361..17263
                     /locus_tag="N7C74_002573"
     CDS             16361..17263
                     /locus_tag="N7C74_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2432418.1"
                     /translation="MKGNVSMAKRENYNELYLFMQVVREGSFTAAAKRLGLAQSGVSR
                     SVRELEERLGVQLLVRTTRKLSLTQAGEQLYQKTASGFEMLDLGLATLAHYRETPSGT
                     VRINASQHAIDKCLLPKLAVFKQRYPDIQLELINESRFVDIIEERFDAGVRLGPEVSQ
                     GMVAVRITPDMEMAIVGTPEHFRRYGFPQTPADLKAHPCIAYQFADGSVYQWELNQDD
                     KKITHQPEGQWAFSDSYMEAEAARLGLGLAYVPVELVADDLEHGKLIRVLQRYSLRME
                     GLFLYYPHRNVSPALRMVIDTLKI"
     gene            17500..17562
                     /locus_tag="N7C74_002574"
     CDS             17500..17562
                     /locus_tag="N7C74_002574"
                     /inference="COORDINATES: protein motif:HMM:NF041474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432419.1"
                     /translation="MNTVVFVATVEYAYLSLHFY"
BASE COUNT         4413 a         4761 c         4664 g         3848 t
ORIGIN      
        1 agaaactgaa tgtacctgta aaaattacag gtttggaaag tagtgacatg gcaaaatgat
       61 tacagcaggg actatgaggt tgaaaaccat atggaatgtc aaaaccgttc tgataaatac
      121 atctggtctc cccatgacgc ctacttctat aaaggactat ctgaactgat tgtggatatc
      181 gacagattaa tttatctatc gctggagaaa atcagaaaag atttcgtgtt tatcaatctc
      241 aatacggatt ctttaactga atttataaac cgtgataatg agtggttatc cgcggtaaag
      301 gggaaacagg tcgtattgat tgcggccaga aagtcagaag ccttagcaaa ttattggtat
      361 tacaacagca atattagggg cgtggtatac gctggactga gtcgtgatat tagaaaagaa
      421 ctggcctatg tgattaatgg caggttcctg agaaaagata ttaagaaaga taaaatcact
      481 gaccgggaaa tggaaattat ccgcatgacg gctcagggaa tgctgcctaa atcgattgcc
      541 agaattgaaa attgtagtgt aaagacagtg tatacccatc ggcgtaatgc agaggccaag
      601 ctgtactcaa aattatataa gttggttcag taaactccag gcaagttagt tttaaaaaat
      661 gactcactgg gacatcacgt cctcaattca actcgggaag aaatacaatg aaaaaaaagg
      721 ttctggcaat agctctggta acggtgttta ccggcatggg tgtggcgcag gctgctgacg
      781 taacagctca ggctgtagcg acctggtcgg caacagccaa aaaagacacc accagtaagc
      841 tggttgtgac gccactcggt agcctggcgt tccagtatgc cgaaggcatt aaaggtttta
      901 actcacagaa aggtctattt gacgtggcta tcgagggtga ctcaacggct accgccttta
      961 aactgacctc acgtcttatc accaacacat taacccagtt ggatacctca ggttccacac
     1021 tgaatgtggg cgtggattat aacggcgcgg cagtcgaaaa aactggcgat accgtgatga
     1081 tcgataccgc caacggcgta ctgggcggca accttagccc gctggcaaac ggttacaatg
     1141 ccagcaatcg taccaccgca caggatggtt tcactttctc catcatcagc ggcaccacca
     1201 atggtaccac cgcagtaacc gattacagca ctctaccgga aggcatctgg agcggcgacg
     1261 ttagcgtaca gttcgacgcg acctggacca gttaatctct ctgatgtacc agcaggggta
     1321 gccccccagc tttattcctg gacggaattg ttatgaaaaa gcaccttctg cttctcgctc
     1381 tgctgttatc cggaatatct ccggcccagg cgctggatgt cggcgatata tcatcgttta
     1441 tgaacagtga cagcagcacg ctgagcaaaa cgatcaaaaa cagtaccgac agtggtcgcc
     1501 ttatcaatat ccgtctcgaa cggctctctt caccgcttga cgacgggcag gttatctcaa
     1561 tggacaagcc ggacgagttg ctactcactc ccgccagctt gctgctaccc gcccaagcca
     1621 gcgaagtgat ccgcttcttc tataagggac cagcagatga aaaagagcgc tactaccgca
     1681 ttgtctggtt tgatcaggcc ctcagtgatg cgcagcgcga taatgccaac cgcagcgctg
     1741 tggccactgc ttccgcccgc atcggcacca ttctggtcgt cgccccccgc caggcaaact
     1801 accactttca gtacgccaac ggcaccctga caaatacagg aaatgcgacg ctgcggatcc
     1861 tcgcctacgg cccctgcctg aaagccgcca atggtaaaga gtgtaaagag aattactacc
     1921 tgatgccggg caagtcgcgt cgttttaccc gcgtggacac ggcggataac aaaggacggg
     1981 ttgcactttg gcagggtgat aagttcattc ccgtgaaata gatagctgtg cagatggata
     2041 acgacaatgc ctttacgacg gttctcccca ggactgaaag cccagtttgc cttcggcatg
     2101 gtctttttgt tcgttcagcc cgatgccagc gctgctgaca taagtgcgca gcaaataggt
     2161 ggggtgatta ttccgcaggc cttcagtcag gcgcttcagg acggcatgag cgtcccgctc
     2221 tatattcatc tcgccggtag ccagggtcgc caggacgatc agcgaatcgg cagcgctttt
     2281 atctggttgg atgatggaca gctacgcatc cggaaaatac agctggaaga gagtgaagat
     2341 aacgccagtg tcagcgaaca aactcgacag cagctgatgg ctctggcgaa cgccccgttc
     2401 aatgaggccc ttaccatccc cctgactgac aacgcgcagc tggatctcag cttgcgccaa
     2461 ctgctgctgc agctggtggt caagcgcgaa gcgctgggca ctgtactacg ctcacgtagc
     2521 gaagacatcg ggcagtccag tgttaacacc ctcagcagta atctgagcta taacttcggc
     2581 atctataaca accagttgcg taacggcggg agcaacacat ccagctatct gtcgctgaat
     2641 aacgttactg cactgcgcga acatcatgtg gtgctcgacg gctcgctgta cgggatcggt
     2701 agcggtcaac aggacagtga attatataaa gcgatgtatg aacgcgattt tgccggtcac
     2761 cgatttgccg gtggaacgct cgacacctgg aacttgcagt ccttagggcc gatgaccgcc
     2821 atttcagcag ggaagattta cggcctttcc tggggaaacc aggccagctc caccatcttc
     2881 gacagcagcc agtcagccac gccagtgatc gcctttttac cggcggcggg tgaagtacat
     2941 ctcacccgtg atgggcggtt actaagcgtt cagaacttca ccatgggcaa tcatgaagtg
     3001 gatacccggg gtctaccata cggtatttac gatgtggaag ttgaggtgat cgttaacggt
     3061 cgcgtgatca gcaaacgcac ccagcgggtc aataagctgt ttagccgggg gcgcggcgtc
     3121 ggtgcaccac tggcgtggca ggtatggggc ggtagctttc atatggatcg ctggtcggaa
     3181 aacgggaaaa agacgcgacc agctaaagag agttggctgg caggtgcctc gacctccggc
     3241 tcactgagta cgcttagctg ggcggcaacg ggatatggat acgataatca ggcggtgggt
     3301 gaaacccgtc tgacgctgcc gcttggggga gcgatcaacg ttaacctgca aaatatgctg
     3361 gccagtgaca gctcatggag cagcatcggc agcatcagcg ccactctacc gggaggcttt
     3421 agttcgctgt gggttaatca ggaaaaaacc cgcattggca atcaattgcg acgtagcgat
     3481 gccgacaacc gtgctatcgg cggcacactc aacctgaact cactgtggtc gaagctgggc
     3541 acattcagca tcagctacaa tgatgaccgc cgttacaaca gccattatta cacggcagat
     3601 tactatcaaa atgtctacag cggtaccttt ggttcgcttg gcctgcgggc cggtattcag
     3661 cgctataaca acggcgacag caacgccaat acagggaaat atatcgctct cgatctctcg
     3721 ctaccactgg gcaactggtt tagcgcaggg atgactcatc aaaacggcta caccatggca
     3781 aacctgtcag cacgcaagca gtttgatgaa gggaccattc gcactgttgg tgccaatctg
     3841 tcacgagcca tctccggcga taccggtgat gacaaaactc tcagtggtgg ggcgtatgca
     3901 cagttcgacg ctcgctacgc cagcggaacg ctgaacgtca atagcgcggc ggacggctac
     3961 gtcaatacca atttaaccgc caatggcagc gtcggctggc agggtaaaaa cattgctgcc
     4021 agcgggcgga ctgatggcaa cgctggggtg atattcaaca ccgggctgga ggacgacggt
     4081 cagatcagcg ccaaaatcaa cgggcggatt ttcccgctta acggcaagcg taactatctc
     4141 ccgctctctc cctatggaag atatgaggtg gagttacaga acagcaaaaa ctcactcgac
     4201 agttacgata tcgtcagcgg tcgcaaaagt catctgactc tctatccagg caatgtcgct
     4261 gtcattgagc cagaggtgaa gcagatggtt accgtctccg gtcgtatccg tgcggaagac
     4321 ggcacactgc tggctaacgc acggattaac aaccatatcg gccgaacccg aaccgatgaa
     4381 aacggcgagt ttgtcatgga cgtggataag aaatacccca ctatcgattt tcgctacagt
     4441 ggcaataaaa cctgcgaagt ggcactggaa ctcaaccagg cgcgcggtgc cgtctgggtc
     4501 ggtgatgtgg tctgcagcgg cctctcatcg tgggcggcgg tgacgcagac aggagaagag
     4561 aatgagagtt aacctactga tagcgatgat aatctttgcg ctaatctggc cagtaactgc
     4621 gctcagagcg gcagtgagca aaacaacctg ggcggatgca ccggcacgcg agtttgtgtt
     4681 tgtcgaaaac aactcagacg acaacttttt cgtcactcct ggcggggcgc tggatccgcg
     4741 cctgaccggt gccaaccgct ggaccggttt aaaatacaat ggttcaggaa ccatctatca
     4801 gcaaagcctc ggctacattg ataacggtta caacaccggc ctttatacca actggaagtt
     4861 tgatatgtgg ctggaaaatt caccagtttc atctccttta actggcttgc gctgcatcaa
     4921 ctggtacgct gggtgtaata tgaccaccag tcttatcctg ccgcaaacca ccgacaccag
     4981 tggattttat ggcgcgacgg tgaccagcgg cggcgcgaag tggatgcacg gcatgttgtc
     5041 agacgcgttt taccagtatc tgcaacaaat gcccgtcggc agcagcttta caatgaccat
     5101 caatgcctgc cagacctctg tgaactatga cgccagcagc ggcgcacgct gtaaggatca
     5161 ggcctccggc aactggtatg ttcgcaacgt cacccatacg aaagcagcaa atctacggtt
     5221 gataaatacc cactcgctgg cggaagtatt tatcaacagc gacggagtac cgactctggg
     5281 cgaagggaac gccgactgcc ggacgcaaac catcggcagc cgttcaggat taagttgtaa
     5341 gatggttaac tataccctgc aaacaaacgg actcagcaac acctcaatcc atatattccc
     5401 ggcgatcgcc aactcgtcgt tagcctcggc cgtcggggcg tacgatatgc agttcagtct
     5461 gaatggcagt tcatggaaac cggtgagcaa tactgcctat tactacacct tcaacgagat
     5521 gaagagcgca gactcgatct atgttttctt ctcgagcaac ttctttaagc agatggtgaa
     5581 ccagggaatc agcgatatca acaccaaaga tctattcaac tttcgctttc agaacaccac
     5641 atcaccggag tctggctggt atgaattttc tacctccaac acgctgatta tcaaaccccg
     5701 tgatttcagc atcagtatta tctccgatga atatactcag acaccgtcgc gggagggata
     5761 tgtcggcagc ggcgagtcgg cactcgattt cggctatatc gtaaccacca gcggtaaaac
     5821 agctgccgac gaagtgctga tcaaggtgac cggccccgcg caggtgattg gcgggcgctc
     5881 ctattgtgtc ttcagctccg atgacggtaa ggcgaaagta ccgttcccgg cgacgctttc
     5941 ctttattacc cgcaacggag ctacaaaaac ctacgatgcc gggtgcgatg atagctggcg
     6001 ggatatgacc gatgcgctgt ggttgaccac accgtggact gatatctctg gcgaagtggg
     6061 gcagatggat aagaccacag tcaaattttc gattccaatg gataacgcca tttctctgcg
     6121 tacggtagat gataacggct ggtttggcga agtcagcgct tcaggagaaa ttcatgttca
     6181 ggcgacgtgg cgtaacatta actaaggccc tgctgacagc ggtctgtatg ctggcggcac
     6241 ctttgacaca ggcgatttcg gtcggcaatc tgactttttc actgccgtcc gagactgact
     6301 ttgtcagcaa acgtgtagtg aataacaaca aaagcgcgcg gatataccgt attgccatca
     6361 gtgctatcga tagcccgggc agcagtgaat tacgcacccg accggtggat ggtgaactgc
     6421 ttttcgcccc ccgccagctg gcgttgcagg ctggtgagag cgagtatttt aaattttact
     6481 atcatgggcc acaggataac cgcgagcgct actaccgggt ctcatttcgc gaggtcccca
     6541 ctcgtaacct gacaaaacgc agccctaccg gcggtgaggt tagcacggag ccggtggtgg
     6601 tgatggatac cattctggta gtacgaccac gtcaggttca gtttaaatgg tccttcgatc
     6661 aggtgaccgg aacggtaagt aacaccggca acacgtggtt taagctactg attaaaccag
     6721 gatgtgattc gaccgaagag gaaggcgatg cctggtatct acgtccggaa gacgtggttc
     6781 atcagcctga gttacgtcag ccggggaatc actatctggt ctataacgac aaattcatta
     6841 agattagcga ttcctgtccg gctaagcccc cttcggcgga ctgatactta tccatcggtc
     6901 gagggaagaa ttccacctca gagactcaaa aatcgtttaa atgatgtgga agcgatcgtc
     6961 aatatcacct gtaacgatag gctttctcat atgcacacca cagtgttcag gagacacgga
     7021 aaggtaccag gcggcctgct aacgagtggt ttcttcacta attaggacgt aaaatcggta
     7081 acggctggaa atcattcaat actcacacta tcgaaagtac accagccaac cgcgatacgt
     7141 ccctgcatac gacgtaccgc agttctcttt acgatttata gctgtactgg tgaattatga
     7201 gcaatctgaa tccatgcatg acgtgtggtg cctgttgtgc atttttccgc gtctctttct
     7261 actgggccga agccgacgat gctggcggca ctattcccgc caggctcact gaacaaatat
     7321 ccccttttca ccgatgcatg agcggaacta atcagaaaaa cccccgatgt attgcccttg
     7381 caggaacccc gggcaaaaat gcctgctgca cgatatataa aaatcgatcg tccacatgca
     7441 gagaattcgc catgtctggt gagaacggag tcgtcaatga ggcttgcaat cgtgcaaggg
     7501 ctaaatacgg gctgacaccg ttataaatat acaataatta tttgcactgc cccgccagct
     7561 gtaatagcgg ggcttcgttt ttcaggggta acaaaccccg atacttcttc tatttgccag
     7621 caacaatgcc tctcttctgt agcgttcttc ggatctacat catcctgaag tagcgcgata
     7681 tactcactga ctatctgaat cagaatataa aaagcaatgt ttttaaccta taaaaatggc
     7741 gctgtatttg cgccattttt atcattcaat gcattatctg tttgagccta aagggatctc
     7801 agggtctggc tcatgagtaa ttctgtttcg taaatctctg cgaataattt caatggacca
     7861 gaaccagact aaatgtccaa aaatttcaga aacattctca taccacggga gatcaaacag
     7921 aggtggtgtc agtcccatga gagggaatga aatcatatga acaaaaagtt gggctaaagc
     7981 acctgccagt aagccctgcc agagtttaat ttttggaaat acttctgcga ccacacaata
     8041 accgacagca aacactatcg aaaagataat gtgcgtaaca ccaacccagt taaagacatg
     8101 cccggcaaag gtataaacag ccgcattggg atctgtcagc cccagccagt ctcgaagaaa
     8161 aatatacgga ggattgagaa aattacgcga gcaatcaatt tgcccagcag ccctgattaa
     8221 tgattccggg ccacacgctg cattaaacat atccaccggg ctacgtggcg gcaatggaac
     8281 ttcagccccc cacttcacaa atgcggaaac aacgccagca atcagcccaa tgaatgcagc
     8341 aagaccataa cgtctgcggt tcggtggagt ttgttcaaat atattcatat ctaccctgct
     8401 tgtaccatta tgttatacac ctctttagga gtattcataa aacaaggtaa atgtaaagaa
     8461 ctgtattgtt ttgtataaca agatggttgt ctaatcacca aagaatataa gcaccatcat
     8521 ttctccctat ttttatatta gaagtgacat agatttgcag ggtgatgcag agctgacaat
     8581 cacaggtttc cttatcggtt tttgcatcgt acaactaaag caataaacca gctcccatca
     8641 tcatcaacat cccggcgaac atgaatttat tagccgggct catagctgca ttcgcgtttt
     8701 aagaataatc ctcctgctgt cgccgactat gcttaacgtt taaaaaagca tcagcactct
     8761 cgcaacgcac tcttattttc ccctttagaa taccggaggc ctggtatgag caaccaaggc
     8821 gaataccccg aagataatcg ggttgggaag catgagccgc acgatttaag tttgacccgt
     8881 cgcgatctga ttaaagtgag cgccgcaacg gcggcggccg ccgtggttta tcctcattct
     8941 acgcttgcgg caagcgttcc ggcagctaca cccgcgcctg agataatgcc cctgacactt
     9001 aaggtgaacg gcaaaaccga gcagcttgag gtggataccc gaaccacgct actggacgct
     9061 ttgcgtgaaa atctgcattt gatcggtacc aagaaaggtt gcgatcacgg acagtgcgga
     9121 gcctgtaccg tgctggtcaa tggtcgcagg cttaatgcct gcctgacgct tgcagtcatg
     9181 catcaggggg ccgagatcac caccattgaa ggcctgggtt caccagataa tcttcacccc
     9241 atgcaggcgg cctttatcaa gcatgatggc ttccagtgcg gctactgcac ctccgggcaa
     9301 atttgctcat cagtagcggt gctaaaagag attcaggacg gcattcccag tcacgtcacg
     9361 gtcgatttgg tttcccctcc agaaagaact gccgatgaga tccgtgaacg tatgagcggc
     9421 aacatctgtc gctgtggtgc atacgctaac atccttgccg ccattgaaga cgctgcgggg
     9481 gagataaaat catgaaggcg tttacctatg aacgagtgaa cacccccgcc gaggcggcac
     9541 ttagcgctca gcgcgtaccc ggcgcaaaat ttatcgcggg cgggaccaat ctgctggacc
     9601 tgatgaagct ggaaattgaa acgcccaccc accttatcga tgtgaacggg ctcgggctcg
     9661 ataagatcga agtgaccgac gcgggtgggc tgcgcatcgg cgcactggta cggaacaccg
     9721 acctggtggc tcacgagcgc gtgcgtcgtg attacgcggt actctcccgc gccctgctcg
     9781 ctggcgcgtc tggtcagtta cgcaatcagg caaccacagc aggtaatctg ctccagcgca
     9841 cgcgctgccc ctatttttac gacaccaatc agccctgcaa taagcgcctg cccgggagcg
     9901 gctgcgcggc gcttgaaggc tttagccgtc agcacgcggt ggtaggcgta agcgaagcct
     9961 gcattgccac ccatccgagc gatatggcgg tcgcaatgcg gttgctggat gcggtggtgg
    10021 aaaccatcac gccggaggga aagactcgca gtatcacact ggctgatttt tatcacccac
    10081 cggggaaaac gccgcacatt gaaaccgccc tgcttcccgg tgagcttatc gttgcggtga
    10141 cgttacctcc gccgctcggc ggaaaacata tctaccgtaa ggtgcgcgat cgcgcctcct
    10201 acgcctttgc cctggtatcg gtcgcggcga ttattcagcc tgacggcagc gggcgcgtcg
    10261 cgctgggcgg agtagcacat aagccctggc gcattgaggc tgcggatgct cagctatccc
    10321 agggggcgca ggccgtatat gacgcgctgt tcgccagcgc ccatcccacc gctgaaaaca
    10381 cctttaaact cctgttggcg aagcgaacgc ttgcctccgt actggctgaa gcgagggcac
    10441 aagcatgaaa tttgataaac ccgcagggga aaacccgatc gatcagctga aggttgtcgg
    10501 tcgtccccat gaccgcatcg acggaccgct gaaaactacc ggcacggcac gctacgccta
    10561 cgaatggcat gaagaatccc ccaacgccgc ctatggctat atcgtcggtt ccgccattgc
    10621 caaaggacgc ctcaccgccc ttgatacgga cgccgcgcaa aaagcgccgg gcgtactgcc
    10681 tgtcattacc gccagtaacg ccggggcact cagtaaaggc gacaaaaaca ccgccaggct
    10741 gttaggcggc cccactattg agcactatca tcaggccatt gctctggtag tggccgagac
    10801 cttcgaacag gcgcgagcgg cggcctcgct ggtgcaggcg cactatcgcc gtaataaagg
    10861 agcttactcc ctggcggacg aaaaacaggc cgtcagtcag ccgccggaag acacgcccga
    10921 caagaacgtc ggtgactttg acggggcttt ctcctccgct gcggtgaaga ttgatgctac
    10981 ctacacgacc ccggaccaga gccatatggc gatggagccg catgcctcga tggccgtctg
    11041 ggatggaaat aagcttactc tctggacctc aaatcagatg attgactggt gccgcaccga
    11101 tctggcaaaa acgctgaaag tgcccgtgga gaatgtgcgt attatctccc cgtatatcgg
    11161 cggagggttt ggcggcaagc tgttcctgag aagcgatgcg ctgctggcgg ccctcgccgc
    11221 ccgagcggtg aaacgtccgg ttaaagtgat gctcccccgc ccctctattc ccaataacac
    11281 cacgcaccgc cccgccaccc ttcagcactt gcgtatcggt gccgaccaga gcgggaaaat
    11341 caccgctatc tcacatgaaa gctggtctgg aaacctgccc ggcggcacgc cggaaacggc
    11401 ggtacagcaa agcgaattac tctacgccgg ggcaaaccgt cataccggcc tgcggctcgc
    11461 cacgcttgat ttgccggaag ggaacgccat gcgtgcgccc ggcgaagccc ccggtctgat
    11521 ggcgctcgaa atcgcgatcg acgaactggc ggaaaaagcg ggcatcgatc ccgtcgagtt
    11581 tcgcatcctg aatgacactc agattgaccc cgccgacccg acgcgccgct tctctcgccg
    11641 tcagcttatc gagtgcttgc gcaccggagc ggataaattt ggttggaagc agcgcaacgc
    11701 cacacccgga caggtgcgcg acggggagtg gctagtcggc cacggcgtcg cggcgggctt
    11761 tcgcaataat ctgctggaaa aatcgggggc tcgggttcac ctcgaaccaa acggcaccgt
    11821 taccgtggaa acggacatga ccgacattgg caccggcagc tacaccattc tggcccagac
    11881 ggcagcggaa atgcttggcg taccgctgga gcaggttgcg gttcacctcg gcgattccag
    11941 tttcccggtt tctgcgggtt ctggtggaca atggggcgcg aatacctcca cctccggcgt
    12001 ttacgccgcc tgtgtgaagc ttcgcgaaat gattgcctcg gcagtcgggt ttgatcctga
    12061 gcagtcgcag tttgccgacg gcaagattac caacggtacc cgaagcgcca tactacatga
    12121 ggccaccgca ggcggcagac tgacagcgga agagagcatt gaattcggaa cactgagcaa
    12181 ggagtaccag cagtcgacct ttgccgggca ttttgtggag gtcggcgtgc atagcgcgac
    12241 gggagaagtt cgggtccggc gtatgctcgc tgtgtgtgct gcaggacgca tcctgaatcc
    12301 gaaaactgca cgcagccaag tcattggcgc aatgactatg ggcatgggcg cggcactgat
    12361 ggaggagctg gcggtggatg accgtttggg ctacttcgtt aatcacgata tggcggggta
    12421 tgaggtgccg gtccatgcgg atatcccaaa acaggaagtg attttcctgg atgataccga
    12481 ccccatatcc tccccgatga aggccaaagg tgtcggtgag ctgggcctgt gcggcgtgag
    12541 cgcggctatc gccaacgcgg tgtataacgc caccggtatt cgggtacgcg attatcccat
    12601 cactctggat aagctgctcg ataagctgcc ggatgtggtt taaggaggaa caatgtcata
    12661 cccgcttttt gataaagacg aacactggca taagccagag caggcgtttc tcaccgatga
    12721 ccaccggacc attctgcgct tcgccgtaga ggcgctaatg tccggtaaag gagcggtgct
    12781 ggtgacgctg gtggagatac gcggcggcgc ggcgcgcccg ctcggggcgc agatggtggt
    12841 gcgcgaagat ggtcgttact gcggttttgt ctctggcggc tgcgtggagg ccgctgccgc
    12901 ttttgaagcg ctggagatga tgggctcagg ctgcgatcgc gaaattcgct atggcgaagg
    12961 ttcgccgtgg tttgacatcg ttctgccctg cggcggtggg atcacgctga cgctccataa
    13021 actacgctcg gcacagcctc tgctcgccgt gctgaaccgc ctggaacaga gaaaaccggc
    13081 ggggctgcgc tacgatccgc aagcacaatc gttggtgtgc ctgcccacgc aaacccgaac
    13141 gggctggaat ctcaatggct ttgaggtggg gttcaggcca tgcgtcaggc tgatgattta
    13201 cggacgttct cttgaggcgc aggcaaccgc gagtcttgca gcagccacag gctatgacag
    13261 ccatatcttc gatctttttc cggcctcagc cagcgctcag atcgataccg atacggcggt
    13321 cattttgctg tgccatgatc tcaaccggga gctgccagtg ttgcaggccg cgcgagaagc
    13381 aaaacccttt tatctcggcg cattgggcag ctatcgaacc cacacgttac gtctgcaaaa
    13441 gctccacgag ctgggatggt ccagggagga gacagcgcaa atccgggcac ccgtcgggat
    13501 attccccaaa gcccggaatg cgcatacact ggccctctcc gtgctggcag aagtcgcctc
    13561 tgtacgtctc catcaggagg aggattcatg cctgcccccg tcgtcctgat ccttgcggcc
    13621 gggcgtggaa agcgctttct cacctccggg ggaaatacca taaattaaga gaaaaatttg
    13681 cagtagccgt tttagaaaat cacctaaatc gtttaaaaga gcgccctacc catagtggtt
    13741 gctcatcgcc ttgtgtaact ttttcagcaa cgcgcgccct cgccggacta aagcgcaccg
    13801 gccttctggc gtgctgtaac aaaggtcagt acgcccgcgc aaaggagcag cacactactc
    13861 atggcaaacg tactctgcca gccaaggcgg tcaaacacga tgcctcctgc cgttgatcca
    13921 agggcgattg agagctgaat caccgcgacc atgagcccgc ctccggcttc ggcgttgtca
    13981 ggcaacgtac gcgcaatcca tgtccaccat ccggttggcg cggcggtggc cagcatgccc
    14041 cagagtccta acagcagcga gaccatccag acgtgatgcc cggtaagcat cagaccgatg
    14101 gcaatgacgg ccatcagtcc gggtatggcc atcagcgtgg ggtagaattt tctgttgaga
    14161 aatatcgaaa caaccagcgt gccaataaaa ccggccacgc cgataatcag caaaatcaac
    14221 gacaagccta aggaattaac ccgtgtcacc gactccagaa atgggcgcac ataggtgaat
    14281 agcgcaaact gccccatgaa aaacaggcca caagctatca tgccgaccac aaccatgcgg
    14341 cggctaaata agcggaatac cgcgccacat gacgtgtggc ttttattcgc atccatgctg
    14401 ggcagactaa tgcactgcca gataaaggcg acaaccgcca tgggcaccaa gcacaagaaa
    14461 gcgcctcgcc atccgaccgt agcccccaga tagcttccca gcggcgcggc cacgaccgtc
    14521 gccagcgcat taccggcgtt gaaaatcgcc agcgcacgcg tgacctgatg ctgcggcacc
    14581 agacgaatcg ccgttgccgc tgacatcgac cagaatccgc caatggccac accaatcatc
    14641 gcacgaccga ccatatacat cagataactg gtagccagcg caataataag ccccgatacg
    14701 gccatcagaa ccgtcatccc cagcagcagg aacttacgat tcatctttcc agctagcgtt
    14761 gaaagcgtca ggctggtcag aaccgccagc gcaccggaga tagcaatccc ccgccctgcc
    14821 agcccctccg tcacgcccag gtcacgggca atcggcgtga gcaggctgac gggcataaat
    14881 tcagaagcaa tcagcacaaa cacgcacagg gtcatggcaa aaataccgcc ccagtatgcg
    14941 tgctgatgat gaggtgatgg tgtttggctt agtgtcgaca tagtgaaatt tcacaaagat
    15001 caggataaaa gccaccggcc atagaccggt ggctttgcat caattcaggg gaggttttat
    15061 ttcagtttgg tctggaaaaa ttgttcgaac ttcgcgaaag gtatttttcc ggccacgtta
    15121 tcgtacaggt ccacgtgatt tgcccctggt accaccacca actctttgtc tttactgcca
    15181 accgccttgt aagcatcttc agcaaaatag cgtgaatgtg ctttctcacc ggtcacaatc
    15241 agcgtcggaa tggtgatttc actggcgtag ctcaacagcg gcatattcat aaatgacagc
    15301 ggcatcgttg ccgtccacgc cccggtagag ttaacggaac gttcgtggaa gccgcgcggc
    15361 atgcgatagt agtcaaagaa ctctttcagt accggatgag gatttgccgg cagcgtttct
    15421 ggcagaatgc gctcgcccgc actgaccttg ccgttgctat cgacattaat atcatgacca
    15481 ccgtgagcga acgttccgct ctgcgcatcc ttccagcgct gttcattcaa atattgcaga
    15541 acagcacgac ggtcggcggt agaataacgg tcttcgccat cccccacacc atggcccatc
    15601 gcccggctca tgtcatacat cacgctggta gcaaccgctt taacgcgggt atccatcgcc
    15661 gcgtcgttca gcgccatgcc gccccagcca caaataccga gcagcccgat acggttgcga
    15721 tctacctctt tttgcagccc taagaaatcc accgccgcgc tgaaatcttc agtgttgata
    15781 tccggtgagg cgacatttcg cggatagccg ccgctttccc cggtgtaaga aggatcgaac
    15841 gccagggtaa caaaccctcg ttccgccagc gtctgcgcat acaagccgct ggattgctct
    15901 ttcaccgcgc caaatggccc actgaccgca atcgccgcca gcttgcgatc gccacggtct
    15961 ttaggcaggt aaagatcgcc cactaaggtg atgccatatc ggttcgggaa cgagacttta
    16021 cggtgatcga ctttctggct ctcggcgaac gttttatccc atttatcggt catggacaca
    16081 ggggcattag gattggttga atcagcataa ctcattgtcg tgactccact taatgatgcg
    16141 aaaagcagca tgaccggcat cgcggttttt agcgttcggg tgaaggcttt cataaaaatc
    16201 cccataaaaa acaacagaga gatacaggtt tccgacggac aacgctgcgg tgctttactt
    16261 tgatggggtc attatagtga gcagaattag tgctgattag ggggcgatta atgctaagat
    16321 taataccata ttgttataaa ttaatgctga gattgccggc atgaagggga atgtttcaat
    16381 ggcaaaaagg gagaactaca acgagctata cctgtttatg caggtggtgc gggaaggcag
    16441 ctttaccgca gcggctaagc ggctgggtct tgctcagtca ggagtcagcc gttctgttcg
    16501 cgagcttgaa gaaaggctgg gcgtccagct gctggtgcgc accacgcgta aactgtcgct
    16561 gacgcaagca ggcgagcagc tctaccagaa gacggcatct ggatttgaaa tgttagatct
    16621 tgggcttgcc acgctggcgc actatcgaga gacgccctcc ggcacggtgc gtatcaatgc
    16681 cagccagcac gcgattgata aatgcctgct gccaaagctg gcggtattta aacagcgata
    16741 tcctgatatc cagcttgaac tgataaatga gagccggttc gtcgatatca tcgaggaaag
    16801 attcgatgca ggtgtccgcc tggggcctga agtcagccag ggcatggtcg cggtacgcat
    16861 cacgcccgat atggagatgg caattgtggg gactccggaa cattttcgtc gctatggttt
    16921 tccgcaaacc ccggcggatt taaaggcgca tccctgtatc gcctatcagt ttgccgacgg
    16981 cagcgtatac cagtgggaac tcaatcagga tgataaaaaa atcactcatc aacccgaagg
    17041 acaatgggcc ttttccgaca gctatatgga agcagaagcc gcccggctgg gcctcgggtt
    17101 ggcctatgtt cctgttgaac tggtcgcgga tgatttagaa cacggtaagc ttatcagagt
    17161 gttgcagcgt tacagcctgc gaatggaggg attgtttctc tattaccctc atcgcaacgt
    17221 ctcccccgct ctgcggatgg tcattgatac attgaaaatc tgaacgttaa caacgaccga
    17281 ctcttcactg tgcaatattg tgtcaggtag tcactctgcg ttcaattcca ggggccgctt
    17341 tgagcgagaa agagcggact tatctgtaca aggggactat ccagcaatgg agatcatcca
    17401 aaagaaaaaa ccacctggct cccctcgaca accttaatcc gggcatttag cctcaatcaa
    17461 cattttcata tctgtcaggt tacttcatta tgaaaaccga tcaataccgt tgtttttgtt
    17521 gcaacagtag aatacgcgta tttatccctc catttctatt aataacggca ataaactgtt
    17581 catttcactg atatttaaaa tatgcatcct tttcaagctg ttcagcggcg taagaaaacg
    17641 tattaaatat gaattcgacg atttaaccat ttctgtctga ttggta
//
LOCUS       DBFIFM010000090        17633 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.090, whole genome shotgun sequence.
ACCESSION   DBFIFM010000090 DBFIFM010000000
VERSION     DBFIFM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.090"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..292
                     /locus_tag="N7C74_002575"
     CDS             <1..292
                     /locus_tag="N7C74_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432420.1"
                     /translation="LVNLLSTPISNIGFNATWGGEKPVYAKEFARWQQLLFNTSMKST
                     LKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSAENRQKRSKK"
     gene            336..5102
                     /gene="ehaG"
                     /locus_tag="N7C74_002576"
     CDS             336..5102
                     /gene="ehaG"
                     /locus_tag="N7C74_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HGU2432421.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNTIALGNGSQALADNAIA
                     IGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLALGSNSSANGINSVALG
                     ADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQLYAISDSVAKRLGGGA
                     AVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTKGKYSAAHGTSSPTASV
                     ITDVADGTISASSKDAVNGSQLKATNDDVEANTANIATNTSNIATNTANIATNTTNIT
                     NLTDSVGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKT
                     TNDAVATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTET
                     TSKITNVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGE
                     DALKWDKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGG
                     NASVNTDGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSAKHG
                     TNGDASVITDVADGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYT
                     IANADYDNVGDALNAIDTTLDDALLWDADAGENGAFSAAHGKDKTASVITNVANGAIS
                     AASSDAINGSQLYTTNKYIADALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALD
                     DNALLWDETANGGAGAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQ
                     NTQIINQLAGNTDATYIQENGAGINYVRTNDDGLAFNDASAQGVGATAIGYNSVAKGD
                     SSVAIGQGSYSDVDTGIALGSSSVSSRVIAKGSRDTSITENGVVIGYDTTDGELLGAL
                     SIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTEEDSLAVGT
                     DSLAMGAKTIVNGDKGIGIGYGAYVDANALNGIAIGSNAQVIHVNSIAIGNGSTTTRG
                     AQTNYTAYNMDAPQNSVGEFSVGSADGQRQITNVAAGSADTDAVNVGQLKVTDAQVSQ
                     NTQSITNLDNRVTNLDSRVTNIENGIGDIVTTGSTKYFKTNTDGVDASAQGKDSVAIG
                     SGSIAAADNSVALGTGSVATEENTISVGSSTNQRRITNVAAGKNATDAVNVAQLKSSE
                     AGGVRYDTKADGSIDYSNITLGGGNGGTTRISNVSAGVNNNDVVNYAQLKQSVQETKQ
                     YTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVA
                     LGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            5470..7125
                     /gene="lldP"
                     /locus_tag="N7C74_002577"
     CDS             5470..7125
                     /gene="lldP"
                     /locus_tag="N7C74_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HGU2432422.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7125..7901
                     /gene="lldR"
                     /locus_tag="N7C74_002578"
     CDS             7125..7901
                     /gene="lldR"
                     /locus_tag="N7C74_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HGU2432423.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTIRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            7898..9088
                     /gene="lldD"
                     /locus_tag="N7C74_002579"
     CDS             7898..9088
                     /gene="lldD"
                     /locus_tag="N7C74_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HGU2432424.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9274..9747
                     /gene="trmL"
                     /locus_tag="N7C74_002580"
     CDS             9274..9747
                     /gene="trmL"
                     /locus_tag="N7C74_002580"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HGU2432425.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(9800..10621)
                     /gene="cysE"
                     /locus_tag="N7C74_002581"
     CDS             complement(9800..10621)
                     /gene="cysE"
                     /locus_tag="N7C74_002581"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HGU2432426.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(10701..11720)
                     /gene="gpsA"
                     /locus_tag="N7C74_002582"
     CDS             complement(10701..11720)
                     /gene="gpsA"
                     /locus_tag="N7C74_002582"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HGU2432427.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(11720..12187)
                     /gene="secB"
                     /locus_tag="N7C74_002583"
     CDS             complement(11720..12187)
                     /gene="secB"
                     /locus_tag="N7C74_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HGU2432428.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12250..12501)
                     /gene="grxC"
                     /locus_tag="N7C74_002584"
     CDS             complement(12250..12501)
                     /gene="grxC"
                     /locus_tag="N7C74_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HGU2432429.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(12643..13074)
                     /locus_tag="N7C74_002585"
     CDS             complement(12643..13074)
                     /locus_tag="N7C74_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGU2432430.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13319..14863
                     /gene="gpmM"
                     /locus_tag="N7C74_002586"
                     /gene_synonym="pgmI"
     CDS             13319..14863
                     /gene="gpmM"
                     /locus_tag="N7C74_002586"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HGU2432431.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            14897..16156
                     /gene="envC"
                     /locus_tag="N7C74_002587"
     CDS             14897..16156
                     /gene="envC"
                     /locus_tag="N7C74_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HGU2432432.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16160..17119
                     /locus_tag="N7C74_002588"
     CDS             16160..17119
                     /locus_tag="N7C74_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HGU2432433.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17106..17633)
                     /gene="waaH"
                     /locus_tag="N7C74_002589"
                     /pseudo
     CDS             complement(17106..17633)
                     /gene="waaH"
                     /locus_tag="N7C74_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
BASE COUNT         4370 a         4577 c         4637 g         4049 t
ORIGIN      
        1 actggtcaac ttattaagta cacctatctc aaatattggt tttaatgcga catggggtgg
       61 tgaaaaacct gtctatgcca aagagtttgc ccggtggcaa caattacttt tcaacacatc
      121 aatgaaatcc acattgaaat tattaccagg tcaatggcaa gacattaatt tgaccctgaa
      181 gggtgtatcg cccaataacc tgggatatct gaagttagcc atcaacatgg aaaatattca
      241 gttcgacaat cttccctctg ctgaaaatcg gcagaaaaga agcaaaaaat aaatgcagtt
      301 agtacgctta taacaaggaa tagcgagcat cattaatgaa caaaatattt aaagttatct
      361 ggaaccctgc gacagggaat tatactgtta ccagcgaaac ggcaaaaagc cgtggcaaga
      421 aatctgggcg cagtaagctg ttaatttctg cgctggttgc gggtggaatg ttgtcgtcgt
      481 ttggggcatt ggcgaatgcc gggaatgaca acggtcaggg tgttgattac ggtagtggat
      541 cagctggcga cggctgggtt gctataggca aaggggcgaa agcaaatact tttatgaaca
      601 ccagtggttc cagtactgct gtgggttatg acgctatagc tgaaggccaa tatagctctg
      661 ccatcgggtc aaaaacccat gcgattggtg gtgcatcaat ggcctttggg gttagtgcaa
      721 tatcagaagg cgatagaagt atagcactgg gtgcctcttc gtattcattg ggccaatact
      781 caatggccct cggccgttat tcaaaagcat tgggtaaatt gtctattgct atgggggact
      841 cttccaaagc ggaaggagca aacgccattg ccctgggaaa tgccactaaa gctactgaga
      901 ttatgagtat tgctcttggc gacaccgcca atgcgtcaaa agcgtattca atggcgctgg
      961 gagcaagtag cgtcgcatct gaagaaaacg ctattgcgat aggtgctgag accgaagccg
     1021 ctgaaaatgc aactgctatt ggcaataatg cgaaggcaaa agggactaat agcatggcaa
     1081 tggggttcgg aagccttgcc gataaagtca atactatcgc attaggaaat ggcagccagg
     1141 ctctggcaga taatgcaatc gccataggcc agggcaacaa agctgatggc gtggatgcca
     1201 tcgctctggg taatggtagc cagtcgagag gcttaaacac cattgcctta ggcacagcca
     1261 gtaatgcaac tggtgataag agtcttgcgc ttggtagtaa tagcagtgcc aacggtatta
     1321 actctgtcgc gctgggcgca gattccattg cggatttaga caataccgtc tctgtcggca
     1381 atagttcatt aaaacgcaag atcgttaatg tgaaaaatgg cgcgatcaag tctgacagtt
     1441 acgatgccat taatggttca cagctttatg ccattagcga ctcggtagca aaaaggcttg
     1501 gaggaggggc tgcagtagat gttgatgacg gtactgttac agcaccaacc tacaatttaa
     1561 aaaatggtag caaaaataac gtaggggctg cgctcgctgt acttgatgaa aacaccctgc
     1621 aatgggacca aaccaaaggc aaatacagcg ctgctcatgg tactagtagc ccaactgcca
     1681 gcgtaatcac cgatgttgcg gatggcacga tttcagcctc cagtaaggat gcggttaacg
     1741 gttcccaact gaaagctacc aatgacgatg tcgaagccaa caccgccaat atcgctacta
     1801 ataccagcaa cattgccacg aatacggcaa atattgccac caataccacc aatatcacca
     1861 acctgacgga ttccgttggt gaccttcagg ctgatgccct gctctggaac gaaactaaaa
     1921 aggcattcag tgcagctcac ggccaggata ccaccagcaa aatcaccaac gttaaagatg
     1981 ccgacctgac ggctgacagc actgatgctg ttaacggctc tcagctgaaa accaccaacg
     2041 atgctgtggc gacgaatacc accaatatcg ccaataacac ttccaatatt gccactaaca
     2101 ccaccaacat ctctaacctg actgagacgg tgactaatct tggtgaggat gcgctgaaat
     2161 gggataagga caatggtgta ttcacggcag ctcatggcac cgagaccacc agcaaaatca
     2221 ccaacgttaa agatggcgac ctgacgactg gcagcaccga tgccgttaac ggctctcagc
     2281 tgaaaaccac caacgatgcc gtggcgacga ataccaccaa tatcgccact aacaccacca
     2341 acatctctaa tctgactgag acggtgacta atcttggtga ggatgcgctg aaatgggata
     2401 aggacaatgg tgtcttcact gcagctcatg gcaacaatac cgccagcaaa atcaccaata
     2461 tcctggacgg cacagtcact gcaaccagtt ccgatgccat taacggtagc cagctttatg
     2521 acttaagcag caatatcgcc acctacttcg gcggcaatgc ttctgtgaat actgacggtg
     2581 tgtttaccgg tccaacctac aaaatcggtg aaacaaatta ttataacgtc ggcgatgcac
     2641 tggctgcgat taactcctca tttagcacgt ctctcggcga tgctctgctt tgggatgcca
     2701 ccgcaggtaa attcagtgcc aaacacggta ctaatggtga cgcaagcgtg atcactgatg
     2761 tcgcagatgg tgaaatttca gactccagtt ctgacgcagt aaacggctca caactccacg
     2821 gcgtgagcag ttatgttgtt gatgcgctgg ggggtggtgc cgaagtcaat gcagacggca
     2881 ccatcactgc gccgacgtac accattgcta atgctgatta cgataatgtc ggtgatgccc
     2941 tgaatgctat cgataccact cttgacgacg ctctgctctg ggatgcggac gccggtgaaa
     3001 atggtgcatt tagcgccgct cacggaaaag ataaaactgc cagtgtaatc actaacgtcg
     3061 ctaacggtgc aatctctgct gccagcagcg acgcgattaa cggctcacaa ctctatacca
     3121 ccaataagta catcgctgat gcgctgggtg gtgacgcaga agtcaacgct gacggcacca
     3181 tcaccgcacc gacttacacc attgcgaacg ccgagtacaa caacgtcggt gacgccctgg
     3241 atgcgcttga tgataacgcc ctgctgtggg atgagactgc caatggcggt gctggagcct
     3301 acaatgccag ccatgacggt aaagccagca tcatcactaa tgtcgctaat ggcagtatta
     3361 gtgaggacag taccgatgca gtgaacggtt ctcagttgaa tgcgacgaat atgatgattg
     3421 agcagaacac ccaaattatc aatcagctcg ctggtaacac cgacgcaacc tatatccaag
     3481 aaaacggtgc gggtattaac tatgtgcgta ctaacgacga cggcttagcg ttcaacgacg
     3541 ccagcgcaca gggtgttggc gctacagcta taggttataa ctctgtcgcc aaaggcgata
     3601 gcagcgtagc tattggtcag ggcagctaca gcgacgttga tacgggtatc gccctgggta
     3661 gcagctctgt ttccagccga gtgattgcca aaggctcccg tgacaccagc ataacggaaa
     3721 atggcgttgt tattggttac gacaccacgg atggcgaact gctcggtgca ttgtctatcg
     3781 gtgatgacgg taaatatcgt caaatcatca acgtagccga tggttccgaa gcccatgacg
     3841 ccgttacggt tcgtcaattg cagaatgcga ttggtgcggt cgcaaccacg ccgactaaat
     3901 acttccacgc taattcaacg gaagaagatt cactggcagt gggaactgac tcgctggcaa
     3961 tgggtgcgaa aaccatcgtg aatggcgata aaggtattgg tatcggttat ggtgcctacg
     4021 tggacgcgaa tgcacttaac ggcattgcca ttggtagcaa tgcgcaagtc attcatgtca
     4081 acagtattgc gataggtaat ggttctacga ccactcgtgg cgctcaaacc aattataccg
     4141 cctacaacat ggacgcaccg cagaactctg tcggtgaatt ctcagtcggt agtgcggatg
     4201 gtcaacgtca gatcactaac gtcgcagcag gttcggctga taccgatgcg gtcaacgtgg
     4261 gtcagttgaa agtaacggat gcgcaggttt cccagaatac ccagagcatt actaacctgg
     4321 ataatcgggt aacgaatctt gattcacgcg tcaccaatat cgaaaacggt attggcgata
     4381 tcgtcaccac cggtagcacc aagtacttca agaccaatac cgatggtgta gatgccagcg
     4441 cgcagggtaa agatagcgtc gcgattggtt ccggctccat tgctgccgct gacaacagcg
     4501 tcgctctggg tacagggtct gtggcaaccg aagaaaatac gatctctgta ggttcctcta
     4561 ctaaccaacg tcgtatcacc aacgtagctg caggtaaaaa tgctaccgat gctgttaacg
     4621 tggcacagtt gaagtcttcc gaagctggcg gtgtacgtta cgacaccaaa gctgatggtt
     4681 ctatcgacta tagcaatatc accctcggtg gcggcaacgg cggtacgact cgtatcagca
     4741 acgtctccgc tggcgtcaac aacaacgacg tggtgaatta cgcgcagttg aagcaaagcg
     4801 tgcaggaaac gaagcaatac accgatcagc gaatggttga gatggataac aaactgtcta
     4861 aaactgaaag caagttgagc ggtggtatcg cttctgcaat ggcaatgacc ggtctgccgc
     4921 aggcttacac tccaggtgcc agcatggcct ctattggtgg cggtacttac aacggtgaat
     4981 cggcagttgc tttaggtgta tcgatggtga gcgccaatgg tcgttgggtc tacaaattac
     5041 aaggtagtac caatagccag ggtgaatact ccgccgcact cggtgccggt attcagtggt
     5101 aatgatccat taagttagtg tgactaaggg caaacgccaa attgcctgat gcgttacgcg
     5161 catcaggcaa gataaggcgc tccgggtcaa caatctaagg gcaattctct gatgaggatt
     5221 gcccttttct ttaccagaca tctcccccca caagaattgg ccctaccaat tcttcgctta
     5281 tctgacctct ggttcacaat tccccaatta aaactcacat caatattgcc aatacataac
     5341 atttagttaa ccattcattg tcattatccc tacacaacac aattggcagt gccactttta
     5401 cacaacgtgt gacaaggaga tgagcaacag actcattaca cgatgtgcgt ggactccagg
     5461 agacctgcaa tgaatctctg gcaacaaaac tacgatcccg ccgggaatat ctggctttcc
     5521 agtttgatag catcgcttcc catcctgttt ttcttctttg cgctgattaa gctcaaactg
     5581 aaaggatacg tcgccgcctc atggacggta gcaatcgccc ttgccgtggc tttgctgttc
     5641 tataaaatgc cggtcgctaa cgcgctggcc tcggtggttt atggcttctt ctatggtttg
     5701 tggcccatcg cgtggatcat tattgcagca gtgttcgtct ataagatctc ggtgaaaacc
     5761 gggcagtttg acatcattcg ctcgtctatt ctttcgataa cccctgacca gcgtctgcaa
     5821 atgctgatcg tcggtttctg tttcggcgct ttccttgaag gagccgcagg ctttggcgca
     5881 ccggtagcaa ttaccgccgc attgctggtc ggcctgggtt ttaaaccgct gtacgccgcc
     5941 ggactgtgcc tgattgttaa caccgcgcca gtggcatttg gcgcgatggg cattccaatc
     6001 ctggttgccg gacaagtaac aggcatcgac agctttgaga ttggccagat ggtggggcgg
     6061 cagctaccgt ttatgaccat tatcgtgctg ttctggatca tggcgattat ggacggctgg
     6121 cgcggtatca aggagacgtg gcctgcggtc gtggttgcgg gcggctcgtt tgccatcgct
     6181 cagtacctta gctctaactt cattgggccg gagctgccgg acattatctc ttcgctggta
     6241 tcactgctct gcctgacgct gttcctcaaa cgctggcagc cagtgcgcgt attccgtttt
     6301 ggtgatttgg gggcgtcaca ggttgatatg acgctagccc acaccggtta cactgcgggt
     6361 caggtgttac gtgcctggac accgttcctg ttcctgacag ctaccgtaac actgtggagt
     6421 atcccgccgt ttaaagccct gttcgcatcg ggtggcgcgc tgtatgagtg ggtgatcaac
     6481 attccggtgc cgtacctcga taaactggtt gcccgtatgc cgccagtggt cagcgaggct
     6541 acagcctatg ccgccgtgtt taagtttgac tggttctctg ccaccggcac cgccattctg
     6601 tttgctgcac tgctctcgat tgtctggctg aagatgaaac cgtctgacgc tatcagcacc
     6661 ttcggcagca cgctgaaaga actggctcta cccatctact ccatcggcat ggtgctggcg
     6721 ttcgccttta tctcgaacta ttccggactg tcatcaacac tggcactggc actggcgcac
     6781 accggtcatg cattcacctt cttctcgccg ttcctcggct ggctgggggt attcctgacc
     6841 gggtcggata cctcatctaa cgccctgttc gccgcgctgc aagccaccgc agcacaacaa
     6901 attggcgtct ctgatctgtt gctggttgcc gccaatacca ccggtggcgt caccggtaag
     6961 atgatctccc cgcaatctat cgctatcgcc tgtgcggcgg taggcctggt gggcaaagag
     7021 tctgatttgt tccgctttac tgtcaaacac agcctgatct tcacctgtat ggtgggcgtg
     7081 atcaccacgc ttcaggctta tgtcttaacg tggatgattc cttaatgatt gttttaccca
     7141 gacgcctgtc agacgaggtt gccgatcgtg tgcgggcgct gattgatgaa aaaaacctgg
     7201 aagcgggcat gaagttgccc gctgagcgcc aactggcgat gcaactcggc gtatcacgta
     7261 attcactacg cgaggcgctg gcaaaactgg tgagcgaagg cgtgctgctc agtcgacgcg
     7321 gcggcgggac gtttattcgc tggcgtcatg acacatggtc ggagcaaaac atcgtccagc
     7381 cgctaaaaac gctgatggcc gatgatccgg attacagttt cgatattctg gaagcccgct
     7441 acgccattga agccagcacc gcatggcatg cggcaatgcg cgccacacct ggcgacaaag
     7501 aaaagattca gctttgcttt gaagcaacgc taagtgaaga cccggatatc gcctcacaag
     7561 cggacgttcg ttttcatctg gcgattgccg aagcctcaca taacatcgtg ctgctgcaaa
     7621 ccatacgcgg tttcttcgat gtcctgcaat cctcagtgaa gcatagccgt cagcggatgt
     7681 atctggtgcc accggttttt tcacaactga ccgaacaaca tcaggctgtc attgacgcca
     7741 tttttgctgg tgatgccgac ggggcgcgta aagcaatgat ggcgcacctt agttttgttc
     7801 acaccaccat gaaacgattc gatgaagatc aggctcgcca cgcacggatt acccgcctgc
     7861 ccggtgagca taatgagcat tcgagggaga aaaacgcatg attatttccg cagccagcga
     7921 ttatcgcgcc gcagcgcaac gcattctgcc gccgttcctg ttccactata tggatggtgg
     7981 tgcatattct gaatacacgc tgcgccgcaa cgtggaagat ttgtcagaag tggcactgcg
     8041 ccagcgtatt ctgaaaaaca tgtctgactt aagcctggaa acgacgctgt ttaatgagaa
     8101 attgtcgatg ccggtagcac tggcaccggt gggtttgtgt ggcatgtatg cgcgtcgtgg
     8161 cgaagttcag gcagccaaag cggcggacgc gcatggcatt ccgtttactc tctcaacggt
     8221 ttccgtttgc ccgattgaag aagtcgcgcc agccatcaag cgcccaatgt ggttccagct
     8281 ttatgtactg cgcgatcgcg gctttatgcg taacgcgctg gagcgagcaa aagcagcggg
     8341 ttgttcgacg ctggttttca ccgtggatat gccgacaccg ggagcgcgtt atcgtgatgc
     8401 gcattctggg atgagcggcc caaatgcggc aatgcgccgc tacttgcaag cggtgacgca
     8461 tccgcaatgg gcgtgggatg tgggcctgaa cggtcgtccg catgatttag gtaatatttc
     8521 ggcttacctc ggcaaaccga ccggactgga agattacatc ggctggctgg ggaataactt
     8581 cgatccgtcc atctcatgga aagaccttga gtggatccgc gatttctggg atggcccgat
     8641 ggtgatcaaa gggatcctcg atccggaaga tgcgcgcgat gcagtacgtt ttggtgctga
     8701 tgggattgta gtttctaacc acggtggccg ccagctggac ggtgtactct cttccgctcg
     8761 tgcgttgccc gctattgcag atgcggtgaa aggtgatatc gccattctgg cggatagcgg
     8821 aattcgtaac gggcttgatg tcgtgcgtat gattgcgctc ggtgccgaca ccgtactgct
     8881 gggtcgtgct ttcctgtatg cgctggcaac agcgggccag gcgggtgtag ctaacctgct
     8941 aaatctgatc gagaaagaga tgaaagtggc aatgacgctg actggcgcga aatcgatcag
     9001 tgaaattacg caagattcgc tggtgcaggg gctgggtaaa gagttgccta cggcactggc
     9061 tccgatggcg aaagggaatg cggcatagtc gtttgagccc cctcacccta accctctccc
     9121 tcagggagtg gggaccgttc ggcgctgtat gtactccctc actctgaaac gacaccgcac
     9181 tctttttttc tccctcgccc ctccggggtg aggggaaaag gccgcactgc tcaccaattt
     9241 tctgctatcc tgcccccgca ctaagggggc agtatgctaa acatcgtact ttacgaacca
     9301 gaaattccgc caaatactgg caatatcatc cgtctttgcg ctaataccgg ctttcgtctg
     9361 catatcatcg aaccgatggg atttgcctgg gacgataagc gcctgcgccg cgcggggctg
     9421 gactatcacg agtttaccgc cgttacgcgt catcatgact ttcgcgcgtt cctcgaagca
     9481 gaaaatcccc agcgcctgtt tgccctcacc acgaaaggta cgcctgctca cagcgccgta
     9541 aactatcagg atggcgatta tctgatgttc ggcccggaaa cacgcggcct gccagcgagc
     9601 attcttgatg ccctgcccgc tgaacaaaaa attcgcattc cgatggtgcc ggacagccgc
     9661 agcatgaacc tgtccaatgc ggtgtcagtt gtggtgtatg aagcctggcg gcagctggga
     9721 tatccgggag cgttgttgag agattagtta ctgtatgccg gatagatgat tacatcgcat
     9781 ccggcacgat cacaggacat tagatcccat ccccatactc aaatgtatgg ttaataccgt
     9841 tgaaatgctg gtccatatcc attgatggct tatcgctgtc tggtttaccg acaatacgag
     9901 ccggaacgcc agcggcggtt gtatgcggcg gcaccggttg cagcaccacg gaacctgcgc
     9961 caatcttcgc gccgcgacca acttcaatat tgccgaggat tttcgcgccc gcgccaatca
    10021 tcacaccttc acgaattttc gggtgacggt caccaccaga tttacccgta ccgccaagcg
    10081 tcacagattg cagaatcgat acgtcgtttt caattaccgc cgtttcaccc acaacgatgc
    10141 ccgtggcgtg gtcgagcata atgccacggc caatttttgc tgccgggtga atatcgacct
    10201 ggaacgtcac agaaacctgg ttttgcagaa agattgccaa tgcgcgacgc ccctgattcc
    10261 acaaccagtg accgatgcga taggcctgca aggcatgaaa acccttcagg tataacaacg
    10321 gggttgagta tttatcgact gccgggtcgc gggtacgcac cgcctgaata tcacaggccg
    10381 cagaggcgat catttccggg tcagcggcgt aggcttcttc caccacttca cggatagcaa
    10441 tagcaggcat aattggcgat gacagcttgt tcgccagcat gtagctcagt gcactaccaa
    10501 ggttttcgtg cttgagtagc gtcgcgtggt aaaaactggc cagcattggc tcacagtccg
    10561 ccagcgttct ggcttcggct ttaatattgt tccagacaat ttccagttct tcacacgaca
    10621 ttgcttactc cacacgatga gataatgacc ggcccgttct gcgcgggccg ggtcatagcg
    10681 gtaacaaagg ttccctgggg ttagtggctg ctgcgctcgt ccttgcgtgc acgacctaat
    10741 aacgtcaatg ctgcctcgcg cgcgtttttt ccgcaatata atacttgata aatttcctcg
    10801 gttattggca tttctacgcc gaagcgatgc gccagttcgc ggacttcttt cgtattgcgg
    10861 tagccttcca ccacctgacc aatcttctcc tgcgcgcttt gtacatccat gccctgaccg
    10921 agcatcatgc caaaacggcg gttacgcgac tggttgtcgg tacaagtaag cacaagatcg
    10981 cccagccccg ccatgcccat aaaggtggca ggatcggcac ccagcgccgc gccaagacgc
    11041 gacatttcag ccagcccacg ggtgatcagt gccgtacgcg cattcgcacc aaaaccgata
    11101 ccgtcggaca tccccgcgcc aatggcaatg acgtttttca ccgcaccgcc aagctgcacg
    11161 ccaatgaaat ccggattgct gtaaacgcgg aaacttttgc cgcagtgcag cagctgctgg
    11221 agatcatcgg caaaggtctg atcggtcgag gccagcgaaa tagctgtcgg taaacctgcc
    11281 gccagttctt tcgcaaacgt tgggccagag ataaccgcca gcggaatttg atcgcctaag
    11341 gcctcacgcg ccacgtcctg taacagacgt ccggtttccg cttccagccc tttggtcgcc
    11401 cacaccagac gcgcatcagg acgcatcagc ggtttaatct ggcgcagcac ttcaccaaag
    11461 acatggctgg gtacgacgac gagaatatta cggctggctg ccagcgcagt ggcgagatcg
    11521 ctttcaagat ggagcgtatc gggaaaaggc acatcgggga gaaacgcggc gttacagcgg
    11581 tcgcgttcaa gcgttgcgat atgttcaggg tcatggcccc agaggacaac ctcgtggcca
    11641 tttcttgcca gggtgatggc aagagcggtg ccgtacgagc cggcaccgat cacagtcatt
    11701 gaagcattac gttggttcat caggcatcct gatgttcttc agtaccttcg ccagcctgct
    11761 gctgcaaata gttcatgaac agcgcatcga agttaaccgg cgcaaggttc agttgcggga
    11821 atgtaccgcg ggataccatg ctggtgatgc actcacgagc atacgggaac agaatgttcg
    11881 ggcagtatgc gcccaggcaa tgtgccatct gggtgccttc gatacccgcg atggagaaaa
    11941 taccgccctg ctgaacttca cacaggaacg cggtttcttc gcccagggag gccgttacgg
    12001 taacacgcag taccacttcg tatacgtcat ctgccagttg ggaagatgcc gtatccagat
    12061 caagtttaac ttctggttgc caatctttct ggaaaacgtg cggcgcgttc ggcgcttcga
    12121 aagagatatc cttggtataa atacgttgga tctggaaagt catttcagtg ttgttttgtt
    12181 ctgacatgtg tagaaaaccc ttaagtgttg tccttaaata ctgcgcaatg taatgccaac
    12241 gttcacacgt tatttcagca ggggatccag tccaccacgt gcatccaatg catacaagtc
    12301 atcacagccg ccaatgtgct gtgcgtcaat aaaaatctgg ggaaccgtgg tgcgaccgct
    12361 gcgtttgatc atctcttcac gcttggcggc attgccatcg atcggcaact cctgaaaact
    12421 cacgcccttg ctgctcagca gtgcttttgc acgatggcaa tacgggcagg tttctttggt
    12481 atagatttca acattggcca taatttagct cctgtttttt taccctgaaa tttcatgttg
    12541 cagggcggct gcaaaatgga gtgtccccag aagcttataa caataagtcg ctgggggcct
    12601 aattgcagct aacggcctgc atcatgaaag acgacaggta aattatttgc cgcgcaccaa
    12661 aggcaagttt tcgccagccc agccagcgac gccttctttc agcacgaata cttgcgcaaa
    12721 accagctttc gtcagtgcgt ttgcaggctc ctggcactgc atgccagaac cgtctaccac
    12781 gataaccggt ttgtctttgt gcttctcaag ctcaccaaca ttgttggctt tgatttcgct
    12841 cggcaacaga ttgatagaac ctgcgatatg gcctttacgg aagtcatcac gctgacgtaa
    12901 atccacaacc acagcgtctt ctttgttgat cagacgcgta gcttcaccac gagtaatcac
    12961 cttcactttc gaggtcaggc tcttaaacgt agtcacaaga accgccacca gtaacgcgat
    13021 ccaggcgata ctcagtatgg gatgacggcc aacaaattgc ataatttctt gcatgggggg
    13081 taacaactcc cgacgtagtg attaaaaaaa ccaggaaagg agtatacctg cgcggtgcgg
    13141 caaatacagc cagcgcgtta actggaatgc aattttgcgg ggcacgacga aaaaaaagcg
    13201 caaatctggc atcggcttgc cgcgagcggt cgtatatttt gatcttcaga ggctatttta
    13261 tcgattcagc tgtagtaaaa ttacgcaaat tttgactctt gagtatgagg ttgtcgcaat
    13321 gtcggtttct aaaaaaccta tggtactggt gattctggat ggctatggct atcgtgaaga
    13381 acagcaggat aacgccattt ttagtgctaa aaccccggta atggatgcac tgtgggccaa
    13441 tcgtccgcat accctgatcg acgcttccgg tctggaagtc ggtctgcctg accgtcagat
    13501 gggtaactcc gaagtaggcc acgttaacct gggtgccggc cgcatcgtgt atcaggacct
    13561 gactcgtctg gacgttgaaa tcaaagatcg cgctttcttt gctaatccag tgctgactgg
    13621 cgcggtagat aaagcgaaaa acgcaggtaa agcagtacac attatgggtc tgctctccgc
    13681 aggcggcgta cacagccacg aagatcacat catggcgatg gtagaactgg cagctgaacg
    13741 cggcgcagaa aaaatctacc tgcacgcatt ccttgacggt cgcgacaccc cgccgcgcag
    13801 tgctgaatcc tcgctgaaaa aatttgaaga gaaatttgct gcgctgggta aaggccgcgt
    13861 agcgtccatc attggtcgtt actacgcgat ggaccgcgat aaccgttggg atcgcgttga
    13921 aaaagcctat gacctgctga ctctggcgca gggtgagttc caggccgata ctgccgttgc
    13981 tggtttgcag gcagcttatg ctcgcgacga aaacgacgag ttcgtgaaag cgaccgttat
    14041 tcgtgcagaa ggtcagccag atgcggcaat ggaagacggc gatgcgctga ttttcatgaa
    14101 cttccgtgct gaccgcgcgc gcgaaatcac tcgtgcattc gtaaacgcag atttcgacgg
    14161 cttcgcgcgt aagaaagtgg ttaacgtcga tttcgtgatg ctgaccgaat acgccgctga
    14221 catcaaaact gcggttgctt acccacccgc ttccctggtt aacaccttcg gcgagtggat
    14281 ggcgaaaaac gacaaaactc agttgcgtat ttccgaaacc gaaaaatatg cccacgttac
    14341 tttcttcttc aacggtggcg tagaagagtc gttcaaaggc gaagatcgca ttctgatcaa
    14401 ctcaccgaaa gtggctacct acgatctgca accggaaatg agctccgcag agctgaccga
    14461 aaaactggtt gcggccatca agagcggcaa atacgacacc attatctgta actatccgaa
    14521 cggcgacatg gtaggtcaca ccggggtgat ggaagcggcg gttaaagcgg ttgaagcact
    14581 ggatcactgc gtggaagaag tcgcgaaagc ggttgagtcc gtgggtggac aactgctgat
    14641 caccgctgac cacggtaacg ctgaacagat gcgcgatccg gcaacgggtc aggcacacac
    14701 ggcacacacc aacctgccag ttccgctgat ttacgttggt gataagaacg tgaaagcggt
    14761 tgcaggcggc aaactttctg acatcgcgcc gaccatgttg tcgctgatgg gtatggaaat
    14821 cccgcaagag atgactggta agccgctgtt catcgtggaa taatccctcc ccatgagggg
    14881 aaaggcgatt aataccatga cacgggccgt gaaaccgcgc aggtttgcaa tcaggcccat
    14941 catctacgcc agcgttctta gcgctggcgt attgttgtgc gccttttccg cccacgcgga
    15001 tgagcgtgac caactcaaat ctattcaggc cgatatcgcc gcaaaagagc gcgcggtacg
    15061 ccaaaagcaa caacaacgcg caagcctgct cgcacaattg aaaaagcagg aagaagcgat
    15121 ctctgaagcc acccgtaagc tgcgcgaaac gcaaaacacg ctcaatcaac tcaataaaca
    15181 gattgatgag atgaacgcgt cgattgccaa actggagcag caaaaagccg cccaggagcg
    15241 cagcctcgcc gcacaactgg atgccgcatt ccgtcagggc gagcataccg gtattcagct
    15301 gattctcagc ggtgaagaaa gccagcgtgg acagcgttta caggcttatt tcggctatct
    15361 caaccaggcg cgacaagaaa ccattgccca gttgaagcaa acgcgtgaag aagtcgccat
    15421 gcagcgtgct gaactggaag agaaacagag cgagcaacaa acgctgttat atgagcagcg
    15481 cgcccaacag gcgaaactga ctcaggcgct gaacgagcgt aaaaagacgc tggcagggct
    15541 ggagtcttcc atccagcaag gtcagcaaca gttgagcgag ctgcgcgcca acgaatcccg
    15601 tctgcgtaac agcattgccc gtgcggaagc cgcggcgaaa gcgcgtgcag aacgagaagc
    15661 acgtgaggcc caggcggttc gcgaccgcca gaaagaagcg acgcgcaaag gcaccaccta
    15721 caaaccgacc gaaagcgaaa aatcgctgat gtcccgaact ggtggcctgg gggcgccgcg
    15781 tggtcaggca ttctggccgg ttcgcgggcc gacgctgcat cgctatggtg aacagctaca
    15841 gggcgaacta cgctggaaag gaatggttat cggtgcctct gaaggtactg aagttaaagc
    15901 gattgccgat ggtcgggtga ttctggctga ctggctgcaa ggttacggtc tggtggtggt
    15961 ggttgagcac ggtaaaggcg acatgagtct ttacggctat aatcagagcg cactggtgag
    16021 cgttggttcg caggttcgcg cgggccagcc aattgcactg gtgggcagca gtggcggtca
    16081 gggtcggcct tcactctatt tcgaaattcg ccgccaaggt caggcggtca atccacagcc
    16141 gtggttggga agataagttt tgtttccatt tcgtcgtaac gttcttgcat ttgccgctct
    16201 gttggcgctc tcctcccccg tacttgctgg caaacttgcc atcgtcattg atgattttgg
    16261 gtatcgcccg cacaacgaaa accaggtgct ggcgatgccc tccgctatct ccgtcgctgt
    16321 attacccgat tcaccgcacg ccagagagat ggcgaccaaa gcgcataaca gcgggcacga
    16381 agtgttgatt catctcccga tggcaccact gagtaaacag ccgctggaga aaaatacgct
    16441 gcgcccggag atgagcagcg acgaaattga gcgcattatt cgtagtgcgg tcaataacgt
    16501 gccctatgcc gtggggatca acaaccacat gggtagcaag atgacctcta acctgtttgg
    16561 tatgcagaaa gtgatgcagg cactggagcg ttacaatctt tacttcctcg acagcgtaac
    16621 catcggtaat acccaggcga tgcgcgccgc gcaaggtact ggcgtgaagg tgatcaaacg
    16681 gaaggtgttc ctcgacgatt cgcagaacga agcggacatc cgtgtgcaat ttaatcgcgc
    16741 aattgatctg gcgcgtcgca acggttcaac cattgccatt gggcatcctc acccttcaac
    16801 ggtacgcgtg ctacaacaga tggtttataa cctgccgcca gacattacgc tggtgaaagc
    16861 cagcagcttg ctgaatgaac cgcaggttga tacttctaca ccgccgaaaa acgctgtgcc
    16921 tgatgcaccg cgtaatccgt tccgtggcgt gaagctgtgc aaaccgaaga aaccgataga
    16981 acctgtttat gctaatcgct tctttgaagt attaagcgaa agcatcagcc agagcacgct
    17041 gatcgtttac ttccagcatc agtggcaagg ctggggcaaa cagcccgaag cggcgaagtt
    17101 taacgctagc gcaaattaaa ggcgctacgc gtaatgcgat ggtgcgaccg cgtttttttc
    17161 gcgccattgc cataagcgga aagaccacag taacgcctgg taaccgacct tcacgctgcg
    17221 cacattggta atcaggcgct tatacatacc ggaagtaaat atctcggcaa tcatccgttg
    17281 gcgggtaaga atatccggct ctttgcgcac cgcatggcaa acacgcaatg cttcgtaagt
    17341 tatttgctga tgaaattccg gataaatcat aattttgtcg gcataatttc gatttaattt
    17401 ctccagcagg cgggtaatct taatatagtg acgttgataa ttaaggtttt tattcccttg
    17461 tctatgcaac cgactcactg acgtattatg cagataatat ttatataatg attgctcggt
    17521 atatcgcgca cgcagcgcgt taaacatgaa ttctgttgtc cagacaatat cctgatgatg
    17581 taatccggca ataaatttaa tgttattttt aacaataaca tcacggcgat aaa
//
LOCUS       DBFIFM010000091        17500 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.091, whole genome shotgun sequence.
ACCESSION   DBFIFM010000091 DBFIFM010000000
VERSION     DBFIFM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.091"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..645)
                     /gene="espY1"
                     /locus_tag="N7C74_002590"
     CDS             complement(<1..645)
                     /gene="espY1"
                     /locus_tag="N7C74_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY1"
                     /protein_id="HGU2432434.1"
                     /translation="MKVSVPGMPATLLNMSNNDIYKMVSGDKMDMKMNIFQRLWETLR
                     HLFWSDKQTEAYKLLFNFVNKKAGNINASKYFTGAVNENEKEKFIHSLELFNELKTCA
                     KNPDEMVAKGNMSWVAQTFGDIELSVTFFIENKEICTQTLQLHKGPGNLGVDLREAYL
                     PGVDMRDCYLGLKTMKGHNKVLYLEPGWNANLDGATLDGATVDGATHLYDEVIII"
     gene            complement(940..1755)
                     /gene="djlA"
                     /locus_tag="N7C74_002591"
     CDS             complement(940..1755)
                     /gene="djlA"
                     /locus_tag="N7C74_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HGU2432435.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            2010..4364
                     /gene="lptD"
                     /locus_tag="N7C74_002592"
     CDS             2010..4364
                     /gene="lptD"
                     /locus_tag="N7C74_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HGU2432436.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINANHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVSIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            4417..5703
                     /gene="surA"
                     /locus_tag="N7C74_002593"
     CDS             4417..5703
                     /gene="surA"
                     /locus_tag="N7C74_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HGU2432437.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            5703..6692
                     /gene="pdxA"
                     /locus_tag="N7C74_002594"
     CDS             5703..6692
                     /gene="pdxA"
                     /locus_tag="N7C74_002594"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HGU2432438.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            6689..7510
                     /gene="rsmA"
                     /locus_tag="N7C74_002595"
     CDS             6689..7510
                     /gene="rsmA"
                     /locus_tag="N7C74_002595"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HGU2432439.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPFQES"
     gene            7513..7890
                     /gene="apaG"
                     /locus_tag="N7C74_002596"
     CDS             7513..7890
                     /gene="apaG"
                     /locus_tag="N7C74_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HGU2432440.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            7897..8745
                     /gene="apaH"
                     /locus_tag="N7C74_002597"
     CDS             7897..8745
                     /gene="apaH"
                     /locus_tag="N7C74_002597"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HGU2432441.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            complement(8742..9056)
                     /locus_tag="N7C74_002598"
     CDS             complement(8742..9056)
                     /locus_tag="N7C74_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HGU2432442.1"
                     /translation="MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFS
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(9059..9292)
                     /locus_tag="N7C74_002599"
     CDS             complement(9059..9292)
                     /locus_tag="N7C74_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HGU2432443.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(9378..9857)
                     /gene="folA"
                     /locus_tag="N7C74_002600"
     CDS             complement(9378..9857)
                     /gene="folA"
                     /locus_tag="N7C74_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HGU2432444.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTRESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(10049..11911)
                     /gene="kefC"
                     /locus_tag="N7C74_002601"
     CDS             complement(10049..11911)
                     /gene="kefC"
                     /locus_tag="N7C74_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HGU2432445.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYICLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(11904..12434)
                     /gene="kefF"
                     /locus_tag="N7C74_002602"
     CDS             complement(11904..12434)
                     /gene="kefF"
                     /locus_tag="N7C74_002602"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HGU2432446.1"
                     /translation="MILIIYAHPYPHHSHANKRMFEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(12542..13873)
                     /locus_tag="N7C74_002603"
     CDS             complement(12542..13873)
                     /locus_tag="N7C74_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2432447.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYILVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(13931..14218)
                     /gene="fixX"
                     /locus_tag="N7C74_002604"
     CDS             complement(13931..14218)
                     /gene="fixX"
                     /locus_tag="N7C74_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HGU2432448.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(14215..15501)
                     /locus_tag="N7C74_002605"
     CDS             complement(14215..15501)
                     /locus_tag="N7C74_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU2432449.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTNVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(15552..16493)
                     /locus_tag="N7C74_002606"
     CDS             complement(15552..16493)
                     /locus_tag="N7C74_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HGU2432450.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYASVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(16508..17278)
                     /locus_tag="N7C74_002607"
     CDS             complement(16508..17278)
                     /locus_tag="N7C74_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HGU2432451.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         4319 a         4841 c         4331 g         4009 t
ORIGIN      
        1 aataataatt acctcatcat ataggtgggt agcaccgtcc acggtagcac cgtccagggt
       61 agcaccgtcc aggttagcat tccacccagg ttccaagtaa aggactttat tgtgaccttt
      121 cattgttttg agaccaaggt aacaatccct catgtcaacg ccgggaaggt aagcctctct
      181 aagatcaacg cccaagttac ctgggccttt atgcagctgc aacgtctgag tacatatctc
      241 cttattttca ataaaaaaag tgacacttaa ctcgatatca ccgaaggtct gtgcgaccca
      301 gctcatattg ccctttgcga ccatctcatc cggatttttt gcgcatgttt tgagttcatt
      361 gaataattcc agactatgga taaacttttc tttctcattc tcgttgacag ccccagtaaa
      421 gtattttgag gcgttgatgt tgccagcctt tttattcacg aaattgaaca gaagtttata
      481 agcctcagtc tgtttatctg accagaacag atgacgtaac gtctcccaca agcgttgaaa
      541 gatattcatc ttcatgtcca tcttgtcccc gctcaccatc ttataaatat cgttattgct
      601 catatttaaa agtgtagccg gcatgcctgg aactgatact ttcatggtaa tagcctcgca
      661 ttgagttaaa aactcatgat gcgcgctctg aataaatata ctttcaaaaa tcgctcattc
      721 agatatgtgt tttttatttt taagacgtaa aaatataatt ccgctcgccg taaagctctc
      781 aaccttaagc agggtcaacg cgataaaacc aacagaaacc ctcccgtaaa ccgtatattg
      841 agcctgtcca ccattctgag taaacgcctt ttctcaaaga agtgaccgcc agggcggcca
      901 ccttttatcg tgggcagtta ctcagcatca tttacagggt catttaaacc ctttctgctg
      961 cttaatcagc tcataggcct gctgaatttc ctgcgctttc tgcttcgcca tctccatcat
     1021 ctcaggcggc aaacctttcg ccaccagctt atcgggatgg tgttcactca tcagcttacg
     1081 gtaggcacgt ttgatggtgg tcgcatcatc cgtcggcttc acgcccagca cattacaggc
     1141 atcttccagc gttgggccac gctgcgcttg ctgccagtta ccaccgccag attgctgctg
     1201 ataaccgccg ccaaactgtg caccgccctg catcatgcgc aaaaactggt caaactgggc
     1261 gcgggagatc cctaattctt cagcaatgac atacagcacc gcccgttcat tcgggtgcag
     1321 tgaaccatca gcaaacgccg cctgaatctg gatctccaga aacatacgaa ttaagtcaaa
     1381 acgaccaaag cagacactgc gaaactggcg catcttttcg cgcagcgggt aattgtctga
     1441 ttttcccacc cggaacgcat tttgcgccgc agtacgggaa gcgccatgaa gattcattcg
     1501 gtccatcaac tggctggcga tatgaatatc agcctccgtg acgcgacctt tggatttggt
     1561 taaatgcccc atcacttcaa aagtggtggc aaaaaacagc gcctgacgct cacgctggtt
     1621 ggcgaaccac gccattttac ggctacgggc tttatcaaac atatggccaa ttaacaggcc
     1681 taacactacg ccccaaaagc cgccgcccat cagtaaggcc acggccacgc caatgatttt
     1741 tccccaatac tgcatatatt ccccaaatcg acacacggat atcagggcta tctcccacaa
     1801 tataaaggtg cttttaccgt tttccggctg cggtcaattg tgggacatcg cctataattt
     1861 gcattatcat acctgtcatt caatgccgtg cctaacacca ctgacgctat tcggacagga
     1921 ttaacactag cgtagagatg acgagtacgt tagtctctga ccgtttgtca cgcgcaacgt
     1981 taccgatgat ggaacaataa aatcaacgta tgaaaaaacg tatccccact ctcctggcca
     2041 ccatgattgc caccgccctt tatagtcaac agggactggc agccgacctc gcctcacagt
     2101 gcatgttggg cgtgccaagc tatgaccgtc ctctggtaca gggcgatacc aatgacttac
     2161 ccgtgactat caatgctaac cacgcgaaag gggactaccc ggatgacgcc gtgtttactg
     2221 gcagcgtgga tatcatgcag ggtaacagcc gtctgcaggc cgacgaagtg cagctccatc
     2281 aaaaagaggc accaggacaa ccggagccgg tacgtaccgt tgatgcgctc ggtaatgtcc
     2341 attacgacga taaccaggtg atcctcaaag ggccgaaagg ctgggcgaat ctgaacacca
     2401 aagataccaa cgtctgggaa ggtgattacc agatggtggg tcgccagggt cgcggtaaag
     2461 cggacctgat gaaacaacgt ggcgaaaacc gctataccat tctggataac ggtagcttta
     2521 cctcctgtct gccgggttct gacacctgga gcgtggtagg tagcgaaatt attcatgacc
     2581 gcgaagaaca agttgcggag atctggaacg cccgctttaa ggtgggtccg gtatcgatct
     2641 tttatagccc ctatttgcag ttgccggtgg gtgacaaacg tcgctctggt ttcttgatcc
     2701 cgaacgccaa gtacaccacc accaactact ttgagttcta cctgccatat tactggaaca
     2761 tcgcgccaaa tatggatgcc accatcacgc cgcattatat gcatcgtcgt ggcaacatca
     2821 tgtgggagaa cgaattccgc tacctctccc aggcgggcgc tggcttgatg gaactggact
     2881 atctgccttc agataaagtc tatgaagatg aacacccgaa cgatgacagt tcacgtcgtt
     2941 ggttattcta ctggaaccac tccggggtca tggatcaggt gtggcgtttc aacgtcgact
     3001 acaccaaggt cagcgatcct agctacttca atgatttcga taacaagtac ggttccagta
     3061 ctgacggcta cgcaacgcaa aaattcagcg ttggctatgc ggtgcaaaac ttcaatgcca
     3121 ccgtttcaac caagcagttc caggttttca gcgaacagaa caccagtagc tactcggcag
     3181 agccgcagtt agacgttaat tactaccaga atgatgttgg tccgtttgat acgcgtattt
     3241 acggccaggc agtgcacttt gttaacacca gagacgacat gcctgaagca acccgtgttc
     3301 acctggaacc gaccatcaat ttgccgctct ctaataactg gggcagcatc aataccgaag
     3361 cgaagttgct ggcaacccat tatcagcaaa ccaatcttga ctggtataac tccagaaaca
     3421 cgaccaagct ggacgaatcc gttaaccgcg taatgccgca attcaaagtt gacggcaaaa
     3481 tggtctttga acgcgatatg gaaatgctgg ctccgggtta tacccaaacg ctggaaccgc
     3541 gcgcgcagta tttgtacgtg ccgtatcgcg atcagagcga catctataac tacgactcgt
     3601 ctctgctgca atctgactac tctggcctgt tccgggaccg gacttacggc ggtcttgacc
     3661 gtattgcctc cgctaaccag gtgacgaccg gtgtcacatc tcgcatatat gatgatgctg
     3721 ccgttgaacg ttttaatatt tccgttggtc aaatctacta tttcacggag tctcgcactg
     3781 gcgatgacaa cataacatgg gagaatgacg acaaaacggg ttcactggtg tgggcaggcg
     3841 atacttactg gcgtatctcc gagcgttggg gattgcgtgg cgggattcag tacgatacac
     3901 gtctggataa cgtagcgacc agtaactcca gcattgaata ccgtcgggat gaagaccgtc
     3961 tggtacagct gaattaccgt tacgccagcc cggaatatat tcaggctacg ctgcctaagt
     4021 actattccac tgctgagcaa tataagaatg gtatttcgca ggtaggtgct gtcgccagct
     4081 ggccaattgc cgatcgttgg tccattgttg gggcctacta ctacgacacc aatgcgaaca
     4141 agcaagccga ctctatgtta ggtgtgcaat acagctcctg ctgctatgca attcgcgtcg
     4201 gttacgagcg gaagctgaac ggttgggata acgataaaca acatgcggta tatgacaacg
     4261 caatcggctt taacatcgaa cttcgcggcc tgagctccaa ctacggtctg ggtacgcaag
     4321 agatgctgcg ttcgaacatt ctgccgtatc aaaacacttt gtgatttgtt gatttaccac
     4381 gtaatccgca gtgcggttaa ttgaaatgga aaaagtatga agaactggaa aacgctgctt
     4441 ctcggtatcg ccatgatcgc gaataccagt ttcgctgccc cccaggtagt cgataaagtc
     4501 gcagccgtcg tcaataacgg cgtcgtgctg gaaagcgacg ttgatggatt aatgcagtcg
     4561 gtaaaactga acgctgctca ggcaaggcag caacttcctg atgacgcgac gctgcgccac
     4621 caaatcatgg aacgtttgat catggatcaa atcatcctgc agatggggca gaaaatggga
     4681 gtgaaaatct ccgatgagca gctggatcag gcgattgcta acatcgcgaa acagaacaac
     4741 atgacgctgg atcagatgcg cagccgtctg gcttacgatg gtctgaacta caacacctat
     4801 cgtaaccaga tccgcaaaga gatgattatc tctgaagtgc gtaacaacga ggtgcgtcgt
     4861 cgcatcacca tcctgccgca ggaagtcgaa tccctggcgc agcaggtggg taaccaaaac
     4921 gacgccagca ctgagctgaa cctgagccac atcctgatcc cgctgccgga aaacccgacc
     4981 tctgatcagg tgaacgaagc ggaaagccag gcgcgcgcca ttgtcgatca ggcgcgtaac
     5041 ggcgctgatt tcggtaagct ggcgattgct cattctgccg accagcaggc gctgaacggc
     5101 ggccagatgg gctggggccg tattcaggag ttgcccggga tcttcgccca ggcattaagc
     5161 accgcgaaga aaggcgacat tgttggcccg attcgttccg gcgttggctt ccatatactg
     5221 aaagttaacg acctgcgcgg cgaaagcaaa aatatctcgg tgaccgaagt tcatgctcgc
     5281 catattctgc tgaaaccgtc gccgatcatg actgacgaac aggctcgtgt gaaactggaa
     5341 cagattgctg ctgatatcaa gagtggtaaa acgacttttg ctgccgcagc gaaagagttc
     5401 tctcaggatc caggctctgc taaccagggc ggtgatctcg gctgggctac accagatatt
     5461 ttcgatccgg ccttccgtga cgccctgacc cgcctgaaca aaggtcaaat gagtgcaccg
     5521 gttcactctt cattcggctg gcatttaatc gaactgctgg atacccgtaa tgtcgataaa
     5581 accgacgctg cgcagaaaga tcgtgcatac cgcatgctga tgaaccgtaa gttctcggaa
     5641 gaagcagcaa gctggatgca ggaacaacgt gccagcgcct acgttaaaat cctgagcaac
     5701 taatggttaa aacccaacgt gttgtgatca ctcccggcga gcccgccggg attggcccgg
     5761 acttagttgt ccagcttgca cagcgtgagt ggccggtcga actggttgtt tgtgccgatg
     5821 ccactctcct taccgaccgg gcagcgatgc tcggtttgcc gctcaccctc cgcccttatt
     5881 cccccaactc ccctgcacaa ccgcaaacta cgggcacatt aacgctactt cctgtcgcgc
     5941 tacgtgaatc tgtcactgcg gggcagttag cgattgaaaa tgggcattac gtggtggaga
     6001 cgctggcgcg agcgtgtgat ggctgtctga acggtgaatt tgctgcgctg atcacaggcc
     6061 ccgtgcataa aggcgtcatt aacgacgcag gcattccgtt taccggtcat accgagtttt
     6121 tcgaagagcg ttcgcaggcg aaaaaagtgg tgatgatgct ggcgaccgaa gaacttcgcg
     6181 tggcgctggc aacgacgcat ttaccgctgc gcgatatcgc agatgctatc acccctgcac
     6241 ttttgcacga agtgattgct attttgcatc acgatttgcg tacaaaattt ggtattgccg
     6301 aaccgcgcat tctggtctgc gggctgaatc cgcacgcggg cgaaggcggt catatgggta
     6361 cggaagagat agacaccatt attcctgtgc tcgacgagct gcgggcgcag gggatgaaac
     6421 tcaacgggcc gctgcctgcc gataccctgt ttcagccgaa atatctcgat aacgccgacg
     6481 ccgtgctggc gatgtaccac gatcagggtc ttcccgtgct aaaataccag ggcttcgggc
     6541 gcggtgtgaa cattacgctg ggcctgccct ttattcgcac atcagtggac cacggcaccg
     6601 cacttgaact ggcgggacgt ggcgaagccg atgtcggcag ttttattacg gcgcttaatc
     6661 tcgccatcaa aatgattgtt aacacccaat gaataatcga gtccaccagg gccacttagc
     6721 ccgtaaacgc ttcgggcaaa actttctcaa cgatcagttc gtgatcgaca gcattgtttc
     6781 tgccattaac ccgcaaaagg gccaggcaat ggtcgaaatc ggccccggtc tggcggcatt
     6841 gaccgagccg gtcggcgaac gtctggacca gctgacggtc atcgaacttg accgcgatct
     6901 ggcggcacgt ctgcaaacgc atccattctt aggcccgaaa ctgacgattt atcagcagga
     6961 tgcgatgacc tttaactttg gcgaactggc cgagaaaatg ggtcagccgc tgcgtgtttt
     7021 cggcaacctg ccttataaca tctccacgcc gttgatgttc catctgttta gctatactga
     7081 tgccattgcc gacatgcact ttatgttgca aaaagaggtg gtgaatcgtc tggttgcagg
     7141 accgaacagc aaagcgtatg gtcgattaag cgtcatggcg caatactatt gcaacgtgat
     7201 cccggtactg gaagttccgc cgtcagcctt tacaccacca cccaaagtgg attccgccgt
     7261 cgtgcgcctg gttcctcatg caacgatgcc tcacccggtt aaagatgttc gtgtgttgag
     7321 ccgcatcacc accgaagcct ttaaccagcg tcgtaaaacc attcgtaaca gcctcggcaa
     7381 cctgtttagc gtcgaggtgt taacgggaat ggggatcgac ccggcgatgc gagcggaaaa
     7441 tatctctgtc gcgcaatatt gccagatggc gaactatctg gcggagaacg cgccttttca
     7501 ggagagttaa cgatgatcaa ttcgccccga gtgtgtattc aggttcaaag cgtctacatt
     7561 gaggcacaat cttcacctga taatgaacgt tacgtttttg cttataccgt aaccatacgc
     7621 aatctggggc gagcgccagt gcagttgttg gggcgttact ggctgatcac caatggcaat
     7681 ggccgtgaaa ccgaagtcca gggcgaagga gtggttggcg tccagccact tatcgcgcct
     7741 ggcgaagagt accagtacac cagcggtgca atcattgaaa ccccgctggg caccatgcag
     7801 ggtcactacg aaatgatcga tgaaaatggc gtccctttca gcatcgacat tcccgtattc
     7861 cgactcgccg ttcccacact cattcattaa aagaatatgg cgacatacct tattggcgac
     7921 gttcatggtt gttacgatga actgatcgca ttgctgcata aagtagaatt tacccctggg
     7981 aaagataccc tctggctgac gggcgatctg gtcgcgcgcg gcccgggttc gctggatgtt
     8041 ctgcgctatg tgaaatcctt aggcgacagc gtacgtctgg tgctgggcaa tcacgatctg
     8101 catctgctgg cagtatttgc cgggatcagc cgcaataaac cgaaagatcg cctgacaccg
     8161 ctgctggaag cgccggatgc cgacgagctg cttaactggc tgcgtcgcca gcctctgctg
     8221 caaatcgacg aagagaaaaa gctggtgatg gcccacgcag ggatcacgcc gcagtgggat
     8281 ctgcagaccg ccaaagagtg cgcgcgcgat gtagaagcgg tgctgtcgag tgactcctat
     8341 cccttctttc tcgacgctat gtacggcgat atgccaaata actggtcacc ggaattgcgg
     8401 gggctgggaa gactgcggtt tatcaccaac gcctttaccc gtatgcgttt ttgcttcccg
     8461 aacggtcaac tggatatgta cagcaaagaa tcgccggaag aggcccctgc cccactgaaa
     8521 ccgtggtttg cgattcctgg acctgtcgct gaagaataca gcatcgcctt tggtcactgg
     8581 gcatcgctgg aaggcaaagg tacgccggaa ggtatttacg cgctggatac cggctgctgc
     8641 tggggtgggt cattaacctg cctgcgctgg gaagataaac agtattttgt ccagccgtcg
     8701 aaccggcata aggatttggg tgagggagag gcggtcgcgt cttaaatccc gtcgagcata
     8761 aaatctaatg ctcctttaat caacgttcgg tgcgcgctgg catcgcaaaa tttcgttccc
     8821 agcgcgttaa ctggaacagt tgcagtctca tgcgtcatga gcacatattc tttgccgtct
     8881 accagtaaaa ggattgggtt aagccgttct ggtgggcgaa tacggctaaa tcgttcaata
     8941 ggtgttaatg gaataacaat acggcgatta atcacaccaa taatgtcgct ggtaacatcg
     9001 ataacaaagg gaaacgcggc gttcctgctg cgactgcggt ataccgtaaa ttgcatcgct
     9061 agaagctccg gtactcatca gagaaacatc cggtttcatc agccaattga tttaacgcag
     9121 cgatagcttc agcgttctct tcacgccaac gtgttgctgc atgtttttta agttcagcat
     9181 tgagcgcaac cgtaagggtg gcgctcatat ttaagcctgc atcacgagcg cgattgacta
     9241 actcacggtc gacggtgacg gtcacacttt gtgtggtacg ttttgcagtc atgatatcgt
     9301 gctcctgtac aatttatacg tctagtatac acggatatct gcacgtatca gtgatgccgg
     9361 aattctaata tacaaaatta ccgccgctcc agaatctcaa aacaatagct gtgagagttc
     9421 tgcgcatcag cgtcgtggaa ttcgctgaat accgattccc agtcatccgg ctcgtaatcc
     9481 ggaaaatggg tgtcgccttc cacttctgcg tcgatatgcg tcaggtacag tttttgcgct
     9541 ttcggcagga actgctcata aacgcgaccg ccgccaatca ccatgatttc tggtacgtca
     9601 ccacacgccg cgatggcttc atccaccgac ttcacccacg ttacgcgatc gtccgtaccc
     9661 ggttgactgc tgaggataat atttttgcgt cctggcaacg gacgaccgat ggattccctg
     9721 gtatggcggc ccataattac gggtttattt aaggtgttgc gtttaaacca ggcgagatcg
     9781 gcaggcaggt tccacggcat ggcgttttcc atgccgataa cgcgatctac cgctaacgcc
     9841 gcaatcagac tgatcattga gatttcccga taaaaaaaat tgtcgccact atacgtaaag
     9901 cgtaaaccgt cgtcgactgg tgcgaggatg atgttgagga aaattttata ttctgctggc
     9961 gagtccacgc tctctccctg gactcgccgc attacaatga aacaaaaaca aacagttagc
    10021 tgtaaagtgt gatttacgtc actctttatt aggatgaggg tttcgtttcc ggttcatccg
    10081 ccatgttgcc ggtatgttta ccttcttctg ttccctgcca gccatgtcgt tgaattaatg
    10141 acagatgttc gcggtcctcg gtgatgatct cacttaacat cgcgctggtg cgtttataga
    10201 ccgccgcgcg ggctttggtg tcattctcaa ccattgccat ctcttccacc atctgaatat
    10261 taaagcggcg gaacacatcg gcacgttctc gcgcttcata cggccccaga cctaaacttt
    10321 ccagcgccag acgcccggtt ttcagcgcac cttcaaaggt ttcacgctct ggcttttcaa
    10381 cgcctgcctg acgcaaacaa atgtagtgat cgacatcgcg ggcgcgggca ataatctgca
    10441 aatgcgggaa atgttctttc accatctccg tcagttgcag gttggtttgc ggatcgtcaa
    10501 tggcgttaat cagcacttcc gctttcgccg ctcccgcaga ttccagtaaa tccatccgcg
    10561 tggcatcgcc ataaaacact ttcataccaa atttacgcag cgtttcgata tggtccggat
    10621 cgtgatcgag caccaccatt ttcaccccgc tggagagcag taaacgtccg gtgatctgcc
    10681 cgaaacgacc gaatccggca atgattaccc gcggctgttc ttcgtcgatc tcatcggctt
    10741 cacgcgcctc ttcagtagaa gattgctcaa ggcgattgag gatcaccagc agaatcggcg
    10801 ttgccgccat cgacagcgcc accgccaggg tcagcgattt cgcccactcc ggctccagca
    10861 cattcgccat ctgcgccgcg ccaaatacca caaaggcaaa ctcactgccc tgccctaaca
    10921 acaccgcaaa ccagcgacgc tgtttatttg gcacctgcaa cggtcgggca atcagccaca
    10981 gcatggcgat tttgatgatg aggaaaccga gcagcaaaat gacaatgcgc aatgggtttt
    11041 caatcagcgt gccaaagtct atcgacatgc caacaccgat gaaaaacagc cccaacagca
    11101 aacctttaaa gggttcgata tcgctttcca gcgcatgacg gtattccgag cttgccagca
    11161 gtacgcccgc cagaaacgcg cccatcgcca tcgacaagcc gacctcttcc agcagcaaac
    11221 caaagccaaa cacaaggaat aacgccacgg cactaaacac ttcccgcaag ccagagcggg
    11281 caacaaaacg cagcgccgga cgcgtgacat agcgccccag cagtaccacc agcaccagcg
    11341 cgcccgccac ttttaacgcc gagagagcaa atgcgcccat cgtcgtcgag gcactgctcg
    11401 ccgccagtag cgggatcatc gccaccagcg ggattgccgc gatatcctgg aacagcagca
    11461 ccgcaaaggc actgcggccc atttgcgtca ccatcagatt gcgttcattc atcgcctgca
    11521 tggcaatcgc cgtagaggag agcgccagcg tcatgccgat caattccgcg acctgccagc
    11581 gcagcccaag taacatacag aacagcccca gcaggccgcc gcaaatcacc atctgcaatg
    11641 cgccaccgcc aaacactgcc gcacgcagct tccacagcct ttgtggatcg agttcgaggc
    11701 cgataataaa cagcatcagc accacgccaa tctcggcaaa gtgcagaata gattcggcat
    11761 cggtcaccag tcgcagcccc cacgggccaa taatgcagcc ggcgatcagg tagccaagta
    11821 ccgatcccag accaagacgt actgcaatgg gtacaatcag cgccgccgaa ccgagataaa
    11881 tcagcgcctg aaccagcgta tggctatcca tgatgggcct cctgccattc cagcagacgt
    11941 tgcttatagt gacgcgcctg cccttcgagg gtttcgtcgt cacaaataaa ggtgcagtgc
    12001 atggcaaacg gtggcagcca gttcagcccg cagtagattg ccgtcgcctg tagcggctgc
    12061 gacagcacat caaagcccgg atgcgcacca atttcaaaat ggctttcccc gccgccggtc
    12121 gtcaccgccc acagcaaatg tttgccatgc agcgccgtgc cgccgtgacc gtaagcccag
    12181 ccgtgcgaga agactttatc gatccaaagt ttaaggagcg gaggaatgct gtaccactgc
    12241 atcgggtgct gccagacgat cagatcggcg cgagacagcg cctcctgctc ggcggcaata
    12301 tcgatattga agtcaggata gagttgataa agagagcgaa tttcgacgcc ttccagcgtc
    12361 cttgcctgtt caaacatccg tttattcgca tgggaatgat gcggatacgg atgcgcataa
    12421 attataagaa tcataggtag cccgcgtgtt tacgcctttg ttttcccaca aagtgtagtc
    12481 atatattcac gcggcttata gtcaataata ttgattcaga aaagagataa atctgaacaa
    12541 attaccccat tctctgcccg cggatcgtca tattgctggt ctgcgccagt gacatccctc
    12601 gagtctccgg ggcaaacgct acggaaatca acaagccaaa cagcgagata cccgccccca
    12661 ttagcatcgt gttactgata ccgtaattat tgataaagat cggtagtgcc caggtcgaaa
    12721 caatggtgcc aatacgactt aaggacataa tcacgcccac ggcagaggcg cggatatccg
    12781 tcgggaagag ttcattagga tagagccact gcaaattacc cggcccgcca gagaaaaagg
    12841 catacaccgc aaaggccatc actaccagcc agatccccat atccgggatc agccccaaaa
    12901 ccgccagcgc cagcgtcatc atggcaaagc tgccaatcaa caatggacgc cgtccggcag
    12961 tgtttagcca cagcatcggc ggaatacagc cgagcataaa gaacaggcta atcaccacgt
    13021 tccccagtgc cgcgtttttg ccaacaccca atcccaacaa accaacgatt tgcgggccaa
    13081 aggtgtaaat ggcgaacatt gggatcacct ggcaggtcca gatggcggca acaaacagaa
    13141 caaaaggaaa atggcggcga ttaaacagat cgcgaaaacg ggtttgctgc ggctgctctt
    13201 catcgaaagc caccggttcg ccaaacagtt tgatcatcat ctcttcgcac tcttttactc
    13261 gccctttgcg taataaccag cggggagatt caggcagttc gaatcgacca atcaaaatca
    13321 acaaacaggg gatcgccgcg ctacccagca tccagcgcca gccgccttcc acatcataaa
    13381 gccagtagcc gaccagatcg gcacaggtcg cgccgacata ccacatggcg gcgataaagc
    13441 tgatggaaaa cgcccgctga cgggtactgg agaactcagt gatcatcgaa gtggcgatgg
    13501 gatagtctgc accgatgaca atgccgataa gtacccgcat caccaacagt tcgacggggg
    13561 atgaaacaaa catcgtcgcc accgatatca cgccgatggc gatgatatca atgaggaaca
    13621 ttttgcgccg tccgacttta tcggaaatat aaccaaacag cgatgtgcca acgaacagcc
    13681 cggcgagcgt tcccgcgccc agcaagccaa tccagtcagc gtccagtttt agcgccggcg
    13741 tcagttgctc cagcgccacg ccaatcatta ccagtatata accatccaga aacggaccac
    13801 cgcttcccca cagcaaaatg cggcggtgaa tagaggaaaa tttaagatcg tcaaagtttc
    13861 tggacggttg catgggttat tcctgttttt tgcaatgaga gtcagtgcgg cgttacgtat
    13921 caaaccaaca tcagccgtaa cggaactcca caccaaaggt gccgcgcggg tattcccact
    13981 gttccagcgc cgaccccagc cccagaatgc gacaggtgcc gcactccaga catccggcgt
    14041 aatcgaagcg cacactgccg tcatcctgct tcttgtacag acctgcgggg cacgctttca
    14101 ccagcagctc cagcgcctgt ttatcggcat cggctttcac aacaatgtgc ggatgctctt
    14161 catcgacatt gaatttattg acgcccagtt tgacgtccac attgacggga gaagtcataa
    14221 aacggtcact cctttcatgc catccttgat cagattgatg aagcccactt tcttgccgtg
    14281 gcggaggatt ttcttgcgca tcagttccgg cgcactgccg tcaatggtga acaggtcacg
    14341 cgccacgcct accgccagtt ccgggtagcc gctaaacatg cgtgggttat caaggaaggc
    14401 cggtagtttc tggtacatac gcatatcgcg cagcgggcca ctctcaagat gctgacgata
    14461 ttccgcgagt ttttgcttac tgaaatcgtc gcttttcatc gctgaaagca cggtttttgc
    14521 tgcagcttcc ccggcggcaa tcgccagatc cataccgcga atggtaaaac cgaggttcat
    14581 acacattccg gcggcatcac cggcaatcaa tacgccgtca ccgaccagct ccggcagcat
    14641 gttgatgcct gcttccggca ctacgtgagc ggaatattcc accagcttgc cacccgcgat
    14701 cagcggtgca acggccggat gttgtttgaa atcttccagc atttgcggca ccgatttttt
    14761 cgcgtcatgc agatgatgca gaccacaaac cagccccagc gacagggtgt tttcattggt
    14821 ataaagaaaa ccgccgccca tcaggccatc ggtgggtgat cccgcaaata ggcaagccgc
    14881 cccctgatta ccctgcaact gaaaacggtc ttcaataacc gacttcggta actcgatcag
    14941 ttccttcacg ccaaccgcca cattcgtcgg tttaacgcgt tttgccatcc ccagcttttc
    15001 ggcaaggatg gagttcactc catcagcaag gatcaccgtt ttcgcttcaa tcacatcgcc
    15061 atcggcttct acaccgacga ctttgccatc gcgctgtacg aggttatcga cgcggatccc
    15121 ggtaattaac tgcgcgcccg cttcttcggc ctgctccatc agccaggcat caaatttact
    15181 gcgcaaaacg gagtaagaac gctgggatgg cgaggtttcg tcaccgttgc agtagtccat
    15241 agtcatcgct gacttttccg tcataaacgc gagtttttca tgggtgatca ggcgttctac
    15301 cggggcggat tcggcgaaac caggaataat gtgttccagg ctgtgggcat agagacgccc
    15361 gccggtgacg ttcttggcac ctgcggaatt gccacgctcg ataactaaca cttgcgcccc
    15421 ttcgcgggcg agcaccagtg cggcaaccga accggcaagc cctgcaccga cgatgatggc
    15481 gtcaaagata tcttcggaca taagagctcc ctgtcagcgg caggaccaaa atgcagccct
    15541 gccagagtgg atcaacgcgc taaagctgcg gtcagcgccg gaaggatctt cacggcgtcg
    15601 ccaacaatgc cgtaatccgc gtactggaag atcggcgcat ttttatcttt attgatggcg
    15661 aaaatggttt gcgacgcgtt agcgccaacc atgtgctgga tctgcccgga gatccccaca
    15721 gctaggtaca gttcaggttt cagcatcagg ttggagatac cgacatagcg ttcgtgctcc
    15781 atccattttt cgttttctgc caccggacga gaacaggcca actccgcacc tatcgccttg
    15841 caaagctgtt ctgccagcgc aatgttctct ttgctgccaa taccgcgacc gacgctgacc
    15901 accaggcggg ctttgtcgag atcgacgctg ttgctctggc gcgcctgggt tgccgtgcgg
    15961 gtaatcgcca cagccggagc ctgccactcc acggtgtgcg tttcgccagt gcgtgacgcg
    16021 tctggctgag ccgcatcgaa cgtgccgctg ctgatggtca gtaccgcata cggcgtggca
    16081 atgcgttctt cgccaatcgc cagaccaccg taaaccatat gtttcactgt tgctttaccg
    16141 tcctgtacgc tgacggtgct ggcatcgtta gacaccgccg ctttaaggcg ataacccagt
    16201 tttgccgcca gtaatttgcc gcgacgggtg tttggcaaca gcaccaggcc gtctgcaccg
    16261 tgctggcgaa tagtgtcagc catgacactg gcgtaatctt cgatcatccg atcgtccggt
    16321 ttgccgttta atttccagac atgattagcg ccgagctgga ttgcctgtgc gccgtcggca
    16381 tcattgagga caaaggcatt gatttgatta gctaaagcct gcgcaccgtt catcagttcc
    16441 ggcagacgag aaggggtatc gctgaatacc cagacttgag aaaacgtgtt catagcatcc
    16501 cctgtaatta aatgactttg cgaagatttt cagcaaatgc ggcgatctgt tcttcgccgt
    16561 cgccttcaat cacgatgcgc tgacgctcgc gctgtttcgg cgcggcaacc tgttgttctg
    16621 accatgctgc ctctgcgtta aaaccgatat ccgccgccga ccatacctgg actggctttt
    16681 tcgccgcgcc gagaatggct ttcatcgaag gaatttgtgg ggagttgata tcagtggaaa
    16741 cagcaacaac cgcaggcagt ggaatgctta aggtttcggt ttcatcttcc agttcgcgct
    16801 caacggtgag ggtatctgcc gtcagggaga taattttgct gacgccgtta actgccggaa
    16861 tattgaggat ttcgcctacc agcaaaccaa cctgctgggc ataaaggtcg gaagaaccat
    16921 cgccacagag gatcaaatca aagcctgctt tctgggcggc tgcagccagt gcgctcgccg
    16981 tttgttgcgg cagtgcctgc tcgaactggt catcaatcac cacaatcagt tcatccggac
    17041 cgcgcgatag cacatcttta cgccctttgg cgttggtcag ggctttaccg cccacactta
    17101 gggccgtcac ctgcgcctct gctgcctgtt gctttagctg gcaagccgct tcaatagcgt
    17161 tgagatcgta ttggcttatt ttggcatcgg ctttactgaa gtctaacgaa ccatcagcat
    17221 tattgaccgc aatatcctgt tcatcaggca cgcacttata gcaagtaata atcttcattg
    17281 catctccaga aatcatgaag gaaacgtcgt tcatatcggt ggtgttaatt acagatatca
    17341 tgccctttaa tttggaaata aaactttaat caccaatatt gaaaatgtca cacgcagata
    17401 aatccaactt tcaatattgt taagttcctc accaatattg aaaacacggc gtagcaaaaa
    17461 gaaattttca atattgtttt atggatcacc aatattgaaa
//
LOCUS       DBFIFM010000092        17204 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.092, whole genome shotgun sequence.
ACCESSION   DBFIFM010000092 DBFIFM010000000
VERSION     DBFIFM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.092"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(244..1050)
                     /gene="trpA"
                     /locus_tag="N7C74_002608"
     CDS             complement(244..1050)
                     /gene="trpA"
                     /locus_tag="N7C74_002608"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HGU2432452.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADTLELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPKKMLAALKAFVQPMKAATRS"
     gene            complement(1050..2243)
                     /gene="trpB"
                     /locus_tag="N7C74_002609"
     CDS             complement(1050..2243)
                     /gene="trpB"
                     /locus_tag="N7C74_002609"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HGU2432453.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2255..3616)
                     /gene="trpCF"
                     /locus_tag="N7C74_002610"
     CDS             complement(2255..3616)
                     /gene="trpCF"
                     /locus_tag="N7C74_002610"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HGU2432454.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVICDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLKMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFADGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVLDKAKVLSLVAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3617..5212)
                     /gene="trpD"
                     /locus_tag="N7C74_002611"
     CDS             complement(3617..5212)
                     /gene="trpD"
                     /locus_tag="N7C74_002611"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HGU2432455.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEHLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5212..6774)
                     /gene="trpE"
                     /locus_tag="N7C74_002612"
     CDS             complement(5212..6774)
                     /gene="trpE"
                     /locus_tag="N7C74_002612"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HGU2432456.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQSLSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVGPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLESRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6796..6889)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6866..6910)
                     /gene="trpL"
                     /locus_tag="N7C74_002613"
     CDS             complement(6866..6910)
                     /gene="trpL"
                     /locus_tag="N7C74_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HGU2432457.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            7048..7929
                     /gene="rnm"
                     /locus_tag="N7C74_002614"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             7048..7929
                     /gene="rnm"
                     /locus_tag="N7C74_002614"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HGU2432458.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAHIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HDGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7926..8546
                     /gene="yciO"
                     /locus_tag="N7C74_002615"
     CDS             7926..8546
                     /gene="yciO"
                     /locus_tag="N7C74_002615"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HGU2432459.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8574..10157
                     /locus_tag="N7C74_002616"
     CDS             8574..10157
                     /locus_tag="N7C74_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HGU2432460.1"
                     /translation="MAGKFRCILLLIVGLFFSSLSYAKNTEMPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESSDPFYREDFTVL
                     YRWPHSLLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTSVD
                     VLRMQKFYLPRKKSSFYRPDTFLQWGGLAILAVILYGNLSPVGWAGMSLVGDMFIMIC
                     WLLPFLFCSLELLFARDDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMP
                     AGYFSAVFLTGYLTGMGYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGE
                     TRKLDYALLGWAVSANLGREWVARITPSLTAAVRAPEIARSGVLFSLQMHLSLGANTS
                     LLGRSYSGGAAGGGGGGGW"
     gene            10370..11242
                     /gene="rluB"
                     /locus_tag="N7C74_002617"
     CDS             10370..11242
                     /gene="rluB"
                     /locus_tag="N7C74_002617"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HGU2432461.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(11282..11872)
                     /gene="cobO"
                     /locus_tag="N7C74_002618"
     CDS             complement(11282..11872)
                     /gene="cobO"
                     /locus_tag="N7C74_002618"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HGU2432462.1"
                     /translation="MSDERYQQRQQRVKEKVDARMAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDLVLLDELTYMVAYDYLPLEEVLQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(11869..12627)
                     /gene="yciK"
                     /locus_tag="N7C74_002619"
     CDS             complement(11869..12627)
                     /gene="yciK"
                     /locus_tag="N7C74_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HGU2432463.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCRSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            12847..13896
                     /gene="sohB"
                     /locus_tag="N7C74_002620"
     CDS             12847..13896
                     /gene="sohB"
                     /locus_tag="N7C74_002620"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HGU2432464.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVSTDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(13932..14183)
                     /gene="yciN"
                     /locus_tag="N7C74_002621"
     CDS             complement(13932..14183)
                     /gene="yciN"
                     /locus_tag="N7C74_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HGU2432465.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            14563..17160
                     /gene="topA"
                     /locus_tag="N7C74_002622"
     CDS             14563..17160
                     /gene="topA"
                     /locus_tag="N7C74_002622"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HGU2432466.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         4162 a         4292 c         4601 g         4149 t
ORIGIN      
        1 ttcttgatat attaacaatt gcctgcggaa taagtgactt agagaaaatt attgatttta
       61 ctggtgttat gttgcgggat taaattgttt gattatatta ctgttgggcg gaaaaatgac
      121 gtaagttgac gttcgacagg ggtaagcgaa acggtaaaaa gataaatatt aaatgaattt
      181 aggacttttc cggcttcgtt aaagaaagtt aaaatgccgc cagcggaact ggcggctgtg
      241 ggattaactg cgcgtcgccg ctttcatcgg ttgtacaaaa gctttcagtg ccgccagcat
      301 tttctttggc tcattaatat gttgctcgat gattttaaca atcgcagaac cagaaatcgc
      361 gcccgcagct cctgcatcaa tcgctgcttt tacctgatcc ggggcggaaa taccaaatcc
      421 ctgcaatgga ggtgccgcgt tgtactcttt cagcttcgca accagatgat tgaggggtaa
      481 cgcggcgcgg ttttctgcgc cggtcacgcc cgctcgtgac agcaaatatg tgtaaccacg
      541 accgtaagag gctatctggc gcagcaggtc gtcatcggca tttggcgggc agatgaagat
      601 aggtgcgaca ttatgacgca acgcggcctg gcggaagggc gcggactctt caactggcac
      661 atcggcaacc agcaccgaat cgacgccgac tttctcgcac tgggcataaa actcatcaat
      721 gcctttgtta aacaccaggt tggcatacat cagcaggcca atgggaatgg tcgggtgttt
      781 ctggcgaatg agtgccagca tctcaaagca ctgcgccggg gtcacacctg ccgcaaaggc
      841 acgcagtgtg gcgttttgaa tcgtcgggcc atctgccagt gggtcggaga aggggatgcc
      901 taactccagc gtgtcagcac cggcttcaat tagcgtatcg ataattttca gcgactgctc
      961 aatgcccgga tcaccgagcg tgacgaaagg aacgaatgcg ccttctttgc gctccttcaa
     1021 ctgggcaaac agagattcgt agcgttccat cagatttccc ctcgtgcttt caaaatatcg
     1081 tgaacggtga agatgtcttt atcgccgcga ccggaaaggt taaccaccag tagctgctct
     1141 ttttccgggt tttcgcgcat cattttcagc gcatgggcca gggcgtggga ggattccagc
     1201 gccgggataa tcccttcgtg caggcacagc gttttgaagg cttccagtgc ttcatcatcg
     1261 gtaattgaca cataatcagc gcgtccagtg ctgttaagat acgcgtgttg tgggccgacg
     1321 gacggaaaat cgagtccggc ggagatggag taagattctt caatctgccc gtcttcggtt
     1381 tgcatcatcg gcgctttcat accgaaatag atacccacgc gaccatgttt tagcggtgcg
     1441 ccgtgctcgc cagtttcgat accgtgacca ccaggctcca caccaatcag gccgacgttg
     1501 gtttcgttga tgaaatctgc aaacatgccg atggcattcg aaccgccgcc aacacaggcg
     1561 ataacggcat ccggcaggcg accttctctt tccagaatct gtgctttggt ttcttcgcca
     1621 atcatccgct gaaactcacg gacaatggtc ggataaggat gtgggccagc tgcggtgccc
     1681 agcatatagt gcgcggtttc ataactgccg gaccagtcgc gcagcgcctc gttacaggca
     1741 tctttcagcg tcgcggaacc gctatgcacc gggatcactt ccgcacccat taaacgcata
     1801 cgaaaaacgt taggcgactg gcgttcaacg tctttggccc ccatataaat acggcatttc
     1861 aggccgagca gggcgctggc aagggccgac gccacgccat gctgaccggc cccggtttcg
     1921 gcgatgattt cggttttacc catccgcttc gccagtaaag cctgaccgag cacctggtta
     1981 gttttatgcg cgccgccgtg cagcaaatct tcgcgcttca gatacagcgt ggtgttcgtg
     2041 ccagccgtaa tgttctggca tttggtcagc gcggttggac gcccggcata gtttttcagc
     2101 aggtcgttga actgagcctg aaattcagga tctttttgcg cgctgacaaa agcttcttcc
     2161 agctggcgca gagcaggcat caggatttgt ggcacgtaca tgccgccaaa ctcaccaaaa
     2221 taggggttaa gtaatgttgt cattgttcct ttccttaata tgcgcgcagc gtctggaaaa
     2281 ccgaggccaa aagacgtgcg tctttgatgc ccggttgcga ctctacagca gaattaaaat
     2341 caagtccggc gcagccggtt tgtgccgctt ccacgcagtt atctgcgcct aagccccccg
     2401 ccagcagaac gttgccaagc gattgaccat ttaatagtga ccagtcgaaa cgttgcccgc
     2461 ttccaccctg gccgttgtct aaaacatatt tatcaacgtg ctgaagctcg cgggcgggca
     2521 gggtttcacc gacgcttaat gctttccaga tggcaacgtg tgctggcaga gcttcacgca
     2581 gcgtatcgat atacagctga tcttcattac catgcagttg cactgccacc agtgataaca
     2641 ccttagcttt gtccagcaca tcggcaatat cgtgattgcg gaacacgcca acatactgca
     2701 acggtgccgc agccatcact tcctgcgcct gttcaacgtt gacgcaacgc ggtgatgtcg
     2761 caacaaaaat caacccaccg taaatcgcgc ccgcgtcgta cgctgcttta gcatcttgcc
     2821 cacgcgtcag gccacatact ttattctcac ccagcaacac ccgacgcacg gcggcgtgca
     2881 aatcgtcatg ggccatcaac gccgaaccaa ttagaaaacc gtcagcgaag tggcttaact
     2941 cgcgcacctg agcgtaagta ttgatgcctg attcgctgat taccgtcacg ttgtgcccca
     3001 gtttcggcgc aagctcgcgg gtacggttga gatcaatcga caaatcacgc agatcgcggt
     3061 tgttgatgcc aacgaccttt gcccccaatg caatggcgcg ctccagttcc tcttcattac
     3121 tgacttcggt cagcacaccc atcttcagac tgtgggcgac ggcggcaagc tggcgatatt
     3181 gttcgtcatc cagtactgaa agcattaata agcaggcatc ggcctggtaa tagcgcgcca
     3241 gatagatctg gtaagggtcg ataatgaagt ctttacataa aatcggctgc ggggcgattt
     3301 ggctgacgat ggggaggaaa tcaaagctcc cctgaaaata tttctcatca gtcagcaccg
     3361 aaattgccga agcgtaatgt ttataaatgg cggcaatgcg tgctggatcg aaatcatcac
     3421 agatcacgcc ttttgacggc gacgctttct tgcactccag aataaacgcc gtgcgcgcac
     3481 cctgtagcgc atcataaaaa tgtcgcgtgc tcggctgaac ctcattctga aaactggcca
     3541 gcggttgctg ctgtttgcgg gcttctaccc aaatcgcctt gtctgcgacg attttcgcta
     3601 aaacggtttg catcatttac cctcgtgccg ccagtgcggt gactctgtcg taagcggaac
     3661 cactgcgcag tacctcaaga acggtttgcg cattggcttg cagatcttca tggccatgca
     3721 ggcgcattaa catggcgaca ttcgccgcga cggctgcttc atgggcggcg tcgcctttac
     3781 cttgtaacaa gcgtgttaaa atgtcacggt tttcttccgg tgttccgcct gccagttgct
     3841 cctggtggta gggagtcagg ccaaaatctt cagcggtcaa ttgatagctc ttaatttcgc
     3901 cgtcatgcag ctcggcaacg attgtcggcg cgtgtaatga aacttcatcc atcccgccgc
     3961 tgtgcaccac cgccgcgcgt tgatacccca gcacgcgtaa ggtttcggca atcggcagca
     4021 ccagttccgg actataaaca ccaattaacg ccagcggcgg atgcgccggg ttaatcaatg
     4081 gccccagcac attgaacagg gtgcgggttt ttagttgctg gcgaaccggc attgcatggc
     4141 gaaatccggt gtgatatttc ggtgcaaaga ggaaacatac acctaactca tccagcgcct
     4201 ggcgcgattt atcggcgttc atatcaagat taataccgaa cgccgccagc agatccgacg
     4261 agccagattt actggagacg ctacggttgc cgtgtttcgc cactttcagc ccacaggccg
     4321 cggcgacaaa cgcactggcg gtggaaatat tgatgctgtt gctgccgtca ccgccggtgc
     4381 cgacgatatc ggcaaacaga taatccggac gcgggaacgg cgcggcgttt tccagtagcg
     4441 cggttgctgc tccggcgatc tcgttcgggt gctcgccgcg aattttcatg ctcaccagcg
     4501 ccgccgccag gtgttccggt ttcagttcac cacgtaccac cgctgaaaat agctgatgac
     4561 tttcctgctg gctaagggtc tgcgcctgat acagtttttc cagaatcggt tgcagcgtgt
     4621 tggttggctc tagtttctgc tgcgcccagg ccagcgtttg ttccagcagg cgagcgccct
     4681 gggtggtgag aatggattcc gggtggaact ggaatccgca aacgcgatct gcatcgtgac
     4741 gcaccgccat caccatgcca ttaaaatggg cgttgatggt taaaccggcc ggaatattac
     4801 tgccaaccag cgagtgataa cgcgccaccg gcagcgggtt tgttaatccg gcaaacatcg
     4861 cctgaccgtc atgttcaatg ctcgacgctt taccgtgaag aatttcgccc gcctgaccga
     4921 catagccccc gtaagcttcg acaatcgcct gatgaccaag gcaaatgcca ataataggca
     4981 gcttaccgcg caggcgggta agcagttcag gcatacaacc agcttcgctc ggcacaccgg
     5041 gtccaggaga aagcatcagc accggattgc tcatcgtcgc aaggcgttca attaaggtct
     5101 gcgccggaat atggttgcgg taaatcacca cgttatgacc attgctgcgc aactgatctg
     5161 ccaggttgta agtaaaagag tcgatattat cgagcagcag aatgtcagcc attagaacgt
     5221 ctcctgtgca tgatgcgcgg tggcaatagc gcgcagtaca gcgcgggctt tattacgggt
     5281 ttcgtcggct tccgactgcg gaacagaatc aaggactacg ccagcaccgg cttgcacggt
     5341 agcgataccg ttttccacca gcgccgagcg gatcacaatg caggtgtcga gatcgccgtg
     5401 cgcggtgaaa taacctaccg cgccgccgta gctgccgcgg cgacgacctt cagcctcggc
     5461 aattaactgc atagcgcgta ctttcggcgc accgcttaac gtccccatat tcatacaggc
     5521 gcggtaagcg tgcagggcgt cgagatcgtg gcgcagctca ccgaccacgc gggagaccag
     5581 gtgcatcacg taagagtaac ggtcaacttt ggtgaggtcg gcgacgtagc ggctgccggg
     5641 ggtgcaaatg cgtgccaggt cattacgggc gagatccacc aacatcagat gttcagaaag
     5701 ctctttatga tcggtacgca tttccagttc aatacggctt tcaaggtccc tgtcgagcga
     5761 gccatcggcg cgacgaccgc gtggacgtgt tccggcaatc gggtagatct caatctggcg
     5821 gctggtggcg tcatacttga gcgaactttc cggcgacgcg ccaaacaggg tgaaatcatt
     5881 atcctgcata aaaaacatgt acgggctggg attactcttt ttcagcacgt aataggccgc
     5941 cagcggtgac gggcagggca gagagaaacg gcgagatggc accacctgga aaatttctcc
     6001 ggcgcgaatc gctttttgca acaaacgcac tacgccaccg aactcttcat cgctctggtt
     6061 acattcacaa cgcatatgcg gcacggaaac caccggcagc ggtggcgcgg tttcggtcag
     6121 ttgctggcga agatcgttca ggcgagcagt gagacgttgt ttttcttctt cattcggagc
     6181 aaacaggctg gcctgaatgc gggtgctttt tttctgatgg tcaatcacca tcagagtttc
     6241 agcgagataa aaacagaaat cagggcagtt attttccgct gacagttgcg gtaaatcttc
     6301 aaaccccgcc acaaggtcat aagagaacag gccgccgaag aacatggctt ctcgttcttc
     6361 cttcggtaca ttcaacagat tctgcaataa gcggaaagcg tcaaaaaccg aaagggagca
     6421 taagcgagcg tcttcatcca gcagtggacc aacagggggg aagcgcagca cacggcagtt
     6481 tggtaattgt tcattttcca cacccgcagg cagggcgtta tccagtagtg tcagcagggc
     6541 ttcgccgttg ccggaaaggg actggattgt gacagtgtca cctaaagctg taatgcgcag
     6601 cgcactgtct accagcagca ggctttttaa atcatctttg ctgtcgatat ctgcggattc
     6661 cagcagcagc gttgccggac gatccccaca caattggtga aaaagcgcgg tgggattgtc
     6721 gcgataagcg ccttcgcagg ttagcagttc gagagtcggt ttttgtgttt gcattgttat
     6781 tctctaattt tgttcaaaaa aaagcccgct cattaggcgg gctgggtatc tgattgcttt
     6841 acgcatggtg aatacactgc ccgtttcagg aagtgcgcca ccaacctttc agtacgaaaa
     6901 ttgctttcat tgtcgatacc ctttttacgt gaacttgcgt actagttaac tagttcgatg
     6961 attgattgtc aacagctcat ttcagaatat ttgccagaac cgttatgatg tcggcgcaaa
     7021 aaacattatc cagaacggga gtgcgccttg agcgacacga attacgcagt gatttacgac
     7081 ctgcacagcc ataccacagc ttccgatggc tgcctgacgc cagaagcatt ggtgcaccgt
     7141 gcagtcgaga tgcgcgtcgg caccctggcg atcaccgacc atgacaccac agctgcaatc
     7201 gcgccagcaa gagaggaaat ttcacgttcg ggactggcgc tgaatcttat tcccggcgtg
     7261 gaaatttcca cggtctggga aaatcatgaa attcatattg tcgggctgaa tattgatatt
     7321 actcatccgc taatgtgtga gttcctcgcg cagcagacag aacggcgcaa tcagcgggcg
     7381 cagcaaattg ccgaaagact tgaaaaagcg catatacctg gggcgctgga aggcgcacaa
     7441 cgactggcgc agggcggcgc agtgacgcgc ggtcattttg cgcgtttcct tgttgagtgc
     7501 ggcaaagcca gttcaatggc ggatgtcttt aaaaagtatc tggcgcgcgg gaaaaccgga
     7561 tacgttccgc cacagtggtg tacaatagaa caagctattg atgtcattca tcattctggc
     7621 ggtaaggcgg tgctagctca tcctgggcgg tacaatctta gtgctaaatg gctgaaaaga
     7681 ttggttgcgc attttgccga acacgacggc gacgcgatgg aagtcgcgca gtgccagcaa
     7741 tcgcccaatg aacgtaccca gctggcgacg cttgcccgcc agcaccattt atgggcatca
     7801 caaggatctg attttcatca gccatgcccg tggatcgaac tgggtcgtaa actctggctg
     7861 cccgcaggcg ttgaaggcgt ctggcagcta tgggaacagc cgcagaacac cacagagagg
     7921 gaattatgag ccagtttttt tatattcatc ctgataaccc gcagcaacgt ctaatcaacc
     7981 aggcggtgga gatcgtgcgt aaaggcgggg tgattgttta tccaactgat tccggctatg
     8041 cgctcggctg taaaattgaa gataaaaacg ccatggagcg tatttgtcgt attcgccagc
     8101 tgccggacgg tcacaacttt accctgatgt gtcgcgatct ttctgaactc tcgacctatt
     8161 catttgtcga taacgttgcg tttcgtttaa tgaaaaacaa cacgccgggc aactatacct
     8221 tcatcctgaa ggggacgaag gaggtgccac gccgtctgtt gcaggaaaaa cgcaaaacca
     8281 tcggtatgcg tgtgccttct aacccgattg cgcaggcgtt gcttgaagca ctgggcgaac
     8341 cgatgctttc cacttcgcta atgctgccag gcagcgaatt taccgaatcc gatcccgaag
     8401 aaatcaaaga ccgtctggaa aaacaggtcg atttgattat tcacggcggc tatctcgggc
     8461 agaaaccaac aacggttatt gatcttaccg atgatacgcc ggtcgtggtg cgtgaaggcg
     8521 taggtgatgt gaagcctttc ttataacaaa ttgcgttcca cggatggaag actatggcag
     8581 ggaagtttcg ctgcattttg ctgttgatag ttgggctttt tttctcttcg ttaagttatg
     8641 cgaaaaacac ggagatgcct tcttatgaag aagggatctc gctctttgat gttgaagcca
     8701 ctctgcaacc ggatggggtg ctcgacatca aagaaaatat tcattttcag gcgcgaaatc
     8761 agcagattaa gcacggattt tatcgtgatt taccacgact atggatgcag cctgatgggg
     8821 acgctgcact gctgaactat catattgttg gcgtcacccg tgatggtatt cctgaaccct
     8881 ggcatcttga ctggcatatc gggttaatga gtattgtcgt gggcgataaa caacgtttct
     8941 tgcctcaagg cgactatcat tatcaaattc attatcaggt taaaaatgct ttcctgcgtg
     9001 agggagattc agatctgtta atctggaacg tgactggtaa ccactggccg tttgaaatct
     9061 ataagacccg attttcactc aagttccctg atatcgcggg taatccattt agcgaaatcg
     9121 atctctttac tggagaagag ggcgacacat atcgaaatgg ccgcatcctt gaggacggaa
     9181 gaattgaatc cagcgatccg ttttatcgtg aagatttcac ggtactctac cgttggcctc
     9241 actcgttact tagcaatgcc ccggctccac aaacgacgaa tattttcagc catcttcttt
     9301 taccctccac gtcatcgttg ttaatttggt ttccgtgtct attcctggtt tgtggatggt
     9361 tatatctctg gaagcgcagg ccgcaattta cgtcggtaga tgttttacgg atgcagaaat
     9421 tttatctgcc gcgtaaaaag tcttcgtttt accggcctga tacttttttg caatggggtg
     9481 gactggcaat attggcggtc attctttacg gtaacctgag tcctgtaggc tgggcaggaa
     9541 tgagtctggt gggcgatatg tttattatga tctgctggct tcttcctttt ttattttgtt
     9601 cccttgagct tttgtttgcc cgcgatgatg acaagccttg cgttaatcgt gtaatcatca
     9661 ctttgttttt accgctgatt tgttcaggcg tggcctttta ttctctctat atcaatgtcg
     9721 gagatgtatt cttttactgg tatatgccag cgggttattt tagcgctgtt ttcctgaccg
     9781 gttatctcac tggcatgggg tatatttttc tgccaaagtt tacccaaact gggcagcaac
     9841 gttatgccca cggtgaagct atcgttaact atctcgcgcg taaagaggca gcaacacaca
     9901 gtgggcgtcg gcggaaaggg gaaacacgga aactggatta cgcgttgcta ggttgggctg
     9961 tctcagcaaa ccttggaaga gaatgggtag cacgtatcac cccatcactc acagcggctg
    10021 ttcgcgcccc ggaaattgcc cgtagtggcg ttttgttctc attacagatg cacctgagtc
    10081 tgggggccaa taccagttta ttggggcgaa gttattccgg tggtgctgcg ggtggcggag
    10141 gcggtggtgg ctggtaatta agctgatgtt aatcgaaaca agctttaacc ataaagatag
    10201 tgtagtatgt tgcgcctcaa agccaggcca gttaaaacgt cgagtcgttt acttaaggcc
    10261 tgaagagttc aaacagactt tactatcagt gagtcagatg taagttctga ctcactgttg
    10321 cacaaaagca tatcagacgc ctgggaaggc gacaccaaaa ggaagctcta tgagcgaaaa
    10381 gctacagaaa gtgctggcgc gtgccggcca cggttctcgc cgtgaaatcg aatctattat
    10441 tgaagccggt cgtgtgagtg ttgatggcaa aattgctaaa ctcggcgatc gcgttgaagt
    10501 tacccctgga ttgaaaatcc gtatcgatgg tcacctgatt tcggtacgtg agtccgctga
    10561 acaaatttgt cgcgttctgg cctattataa accggaaggt gagttgtgta cccgtaacga
    10621 tccggaaggg cgtccaacgg tgtttgaccg tctgccaaag ctgcgcggtg cacgctggat
    10681 tgccgtaggt cgtctggacg ttaacacctg tggtctgttg ctgttcacca ccgacggtga
    10741 actggctaac cgtttaatgc atccaagccg tgaagttgaa cgtgaatatg ccgtgcgtgt
    10801 atttggtcag gttgacgacg cgaaactgcg tgatttaagc cgtggtgtgc agctggaaga
    10861 tggtccggca gcctttaaaa ccatcaagtt cagcggcggc gaagggatca accagtggta
    10921 caacgtgact ctgaccgaag gccgtaaccg tgaagttcgt cgtctgtggg aagcggttgg
    10981 cgtgcaggta agccgcctga tccgtgttcg ttacggtgat atcccactgc cgaaaggtct
    11041 gccacgcggc ggctggacgg agctggatct tgcccagact aactatctgc gcgaactggt
    11101 ggagttgccg ccagaaacca gctctaaagt cgctgtagaa aaagaccgtc gtcgcatgaa
    11161 ggcgaatcag attcgtcgtg cggtgaaacg tcacagtcag gtgagcggcg gacgtcgttc
    11221 tggtgggcgt aataacggtt aatcaacgta tgccggacgt catatccggc atttttacag
    11281 attagtaatc aatccctatc tgcgctttta cgccagcatc aaacgcatgt ttcacggggc
    11341 gtaactcact taccgtatcc gccagttcga gtatatcccg atgacagccc cgacccgtga
    11401 taatcaccgt ctgctgatgt ggacgttcat ttaacgcctg caacacttct tccagtggca
    11461 aatagtcata cgccaccata taagtcagct catcaagcaa aaccaaatcc agtgaggaat
    11521 cagcaagcat ccgctttgca tgttgccaga cttcgcggca ggcggcggta tcagactcgc
    11581 ggttttgtgt atcccaggta aagcccgttg ccatcacctg aaactcaacg ccatgtggct
    11641 ccagcagatt gcgttcgcca ttaggccagg tgcctttaat aaactgcacg acgcccactt
    11701 tttttccgtg accaactgcg cgtgttgccg taccaaatgc cgcggtggtt ttgccttttc
    11761 cattgccggt aaagacgatg ataataccgc gttcatcctg ggcctgggcc atacgagcat
    11821 ctactttttc tttcactcgc tgctgacgct gttggtagcg ttcatcactc attgggaaat
    11881 tcctggttta cggcccggtt gggcgtcaaa ggtcatgccg gttttacggc ggctgtcatc
    11941 gcccatcagc cagaggtaga gcggcatgat atcagcgggt gttttaagtt tctgtggatc
    12001 ttcggtcggg aaggcgctgg cacgcattgc ggtgcgcgta ccgcctgggt taatgcagtt
    12061 gacacgcagg cgctgctgat attcatcggc cagtacctgc atcatccctt cggtggcaaa
    12121 tttcgacgct gcatatgcac cccagttggc tcgtccctga cgtccaacgc ttgatgaagt
    12181 aaagaccagt gaaccggcgt ccgatttgag taataaagga agtagtgcct gggtgagcat
    12241 aaaggtggca ttaacgttga cctgtatgac gtcctgccag acctgtggat tttgttcgct
    12301 cattggacaa acatcgccga gtaatccggc attatgcaaa acaccatcca gacgcggata
    12361 attaacggca atgcgctgtg ccagttgttg gcaatcttcg gacctgcagg tcagcaaatc
    12421 gagaataaac cactgtggct gacgcccagt ttcttcgttt atgtggctgg ctacctgacg
    12481 taatttttct tcattacggc ccaacagaat cactgtcgca ccatagcgtg catacgtcat
    12541 cgcggcttca cgaccaatac catcactggc tcccgtcacc aggataatgc gatcattgag
    12601 taaatcttgt tttggctggt aatgcatggc tactcctcaa cgacgttgtc tgtcgcgctg
    12661 ccagtgtact ttatgacttt ggggctttat gcctgaaaca gaagggcatt tcaatcagcc
    12721 tcgggcgata acaccgcaaa atggatactt tgtcatactt tcgctgcaat aacatctctg
    12781 cgagacggct taacatgcct gttgtaaact gtgagccaaa gcgttgttta accaaggtgg
    12841 ggactcgtgg aattgttgtc tgaatatggt ttgtttttgg cgaaaatcgt taccgttgtg
    12901 ctagcgattg cggcgattgc cgccattatt gtcaatgttg ctcaacgtaa taaacgccag
    12961 cgtggcgagt tacgggtcaa caatctcagc gaacagtata aggagatgaa agaagaactg
    13021 gccgcggcgc tgatggactc acatcagcaa aaacagtggc acaaagcgca gaagaaaaag
    13081 cacaagcaag aagcgaaagc agcaaaagcg aaagccaaac tgggcgaggt gtcaactgac
    13141 agtaaacccc gcgtctgggt gctggacttt aaaggcagca tggacgccca tgaagtgaac
    13201 tcgctacgtg aagagataac ggcggtactt gcagcattca aaccgcagga tcaggttgtg
    13261 ctccgtctgg aaagccctgg tggcatggtg catggttacg ggttggcggc ttcgcagctg
    13321 cagcgtctgc gtgataaaaa cattccatta actgttacgg tagacaaagt tgctgccagc
    13381 ggtggttaca tgatggcctg tgtggcggac aaaattgttt ccgcaccgtt tgctattgtg
    13441 ggttccattg gggtggtggc gcaaatgccc aactttaacc gcttcctgaa aagcaaagat
    13501 attgatatcg aactgcacac cgccgggcag tataagcgta cgctgacctt gctgggtgaa
    13561 aataccgaag aagggcggga gaaattccgc gaagagctga acgaaacgca tcagttattt
    13621 aaagattttg tgaagcgtat gcgtccgtct ctggatattg aacaggtggc aacgggtgaa
    13681 cactggtacg gacaacaggc ggtagagaag ggcctggttg atgaaatcaa caccagtgat
    13741 gaagttattc ttagcctgat ggaaggccga gaagtggtca atgtacgcta tatgcagcgt
    13801 aaacgactca ttgaccgatt caccggcagc gcggcagaga gcgccgatcg attattgcta
    13861 cgctggtggc agcgtgggca aaagccattg atgtaaaaga caaacgcgag ggtaagacct
    13921 cgcgttttgc tttaatcaac cagatgatat ttttctgaaa gcacatgggc caggtgtttg
    13981 aacatattaa acaccgcggt gcttttggct gttggcaatc cttgttcatc taaaaagtag
    14041 tcgccggtaa ataccagcac gccattacgc tgtgtgacgc cggtggcttc aatccctgcg
    14101 agcgtatcct catgctcacg aatgattttg ttggcctctt tcaacagcgt ttcgcggtcg
    14161 atgggttgtg tctctttgtt cattatttac tccttaaaca aggacattag tctacgccag
    14221 gcatggcttg cagacaaata taccacgctg gtggcaagag cgccttactg gcaactttgg
    14281 attttgcatg ctaataaagt tgcgtatcgg attttatcag gtacagtgtg acgctttcgt
    14341 caatctggca atagatttgc ttgacattcg accaaaattt cgtcgtgcta tagcgcctgt
    14401 aggccaagac ctgttaactc agtcacctga attttcgtga acagagtcac gacaaggggt
    14461 tgatatccgc agagagcgag tccatatcag taactcgttg ccagtggaag gtttatcaac
    14521 gtgcgacgca ttcctggaag aatcaaatta ggtaaggtga atatgggtaa agctcttgtc
    14581 atcgttgagt ccccggcaaa agccaaaacg atcaacaagt atctgggtag tgactacgtg
    14641 gtgaaatcca gcgtcggtca catccgcgat ttgccgacca gtggctcagc tgccaaaaag
    14701 agtgccgact ctacctccac caagacggct aaaaagccta aaaaggatga acgtggcgct
    14761 ctcgtcaacc gtatgggggt tgacccgtgg cacaattggg aggcgcacta tgaagtgttg
    14821 cctggtaaag agaaggtcgt ctctgaactg aaacaactgg ctgaaaaagc cgaccacatc
    14881 tatctcgcaa ccgaccttga ccgcgaaggg gaagccattg catggcacct gcgggaagtg
    14941 attggtggtg atgatgcgcg ctatagccga gtggtgttta acgaaattac taaaaacgcg
    15001 atccgccagg catttaacaa accgggtgag ctgaatattg atcgtgttaa tgcccagcag
    15061 gcgcgtcgct ttatggaccg cgtggtgggg tatatggttt cgccgctgct atggaaaaag
    15121 atcgctcgtg gtctgtctgc cggtcgtgtg cagtcggtgg cagtccgcct ggtggtcgag
    15181 cgtgagcgtg aaattaaagc gttcgtgccg gaagagttct gggaagtcga tgccagcacg
    15241 accacgccat ctggtgaagc gttggcgttg caggtgactc atcagaacga caaaccgttc
    15301 cgtccggtca acaaagaaca aactcaggct gcggtaagtc tgctggaaaa agcgcgctac
    15361 agcgtgctgg aacgtgaaga caaaccgaca accagtaaac ctggcgctcc ttttattacc
    15421 tctacgctgc aacaagctgc cagcacccgt cttggatttg gcgtgaaaaa aaccatgatg
    15481 atggcgcagc gtttgtatga agcaggctat atcacttaca tgcgtaccga ctccactaac
    15541 ctgagtcagg acgcggtaaa tatggttcgc ggttatatca gcgataattt tggtaagaaa
    15601 tatctgccgg aaagtccgaa tcagtacgcc agcaaagaaa actcacagga agcgcacgaa
    15661 gcgattcgtc cttctgacgt caatgtgatg gcggaatcgc tgaaggatat ggaagcagat
    15721 gcgcagaaac tgtaccagtt aatctggcgt cagttcgttg cctgccagat gaccccagcg
    15781 aaatatgact ccacgacgct gaccgttggt gcgggcgatt tccgcctgaa agcacgcggt
    15841 cgtattttgc gctttgatgg ctggacgaaa gtgatgcctg cactgcgtaa aggcgatgaa
    15901 gatcgtatct tacctgcagt cgataaaggc gatgctctga cgctcgttga actgacacca
    15961 gcccagcact ttaccaagcc gccagcccgt ttcagtgaag catcgctggt taaagaactg
    16021 gaaaaacgtg gtatcggtcg tccgtctacc tatgcgtcga tcatttcgac cattcaggat
    16081 cgtggctatg tgcgagtaga aaatcgtcgt ttctatgcgg aaaaaatggg cgaaatcgtc
    16141 accgatcgcc tggaagagaa tttccgcgag ttaatgaact acgacttcac cgcgcagatg
    16201 gaaaacagcc ttgaccaggt ggcaaatcac gaagcagagt ggaaagctgt actggatcac
    16261 ttcttctcgg atttcactca gcagttagat aaagctgaaa aagatccgga agaggggggt
    16321 atgcgtccga accagatggt tctgaccagc atcgactgcc cgacctgtgg tcgcaaaatg
    16381 gggattcgca cagcgagcac cggggtattc cttggctgtt ctggctatgc gctgccgccg
    16441 aaagagcgtt gcaaaacaac cattaacctg gtgccggaaa acgaagtgct gaacgtgctg
    16501 gaaggcgaag acgctgaaac caacgcgctg cgcgcaaaac gtcgttgccc caaatgcggc
    16561 acggcgatgg acagctatct catcgatccg aaacgtaagt tgcatgtctg tggtaataac
    16621 ccaacctgcg acggttacga gatcgaagag ggcgaattcc gcattaaagg ttatgacggc
    16681 ccgatcgttg agtgtgaaaa atgtggttct gaaatgcacc tgaaaatggg gcgtttcggt
    16741 aaatatatgg cctgcaccaa cgaagagtgt aaaaacacgc gtaagatttt acgtaacggc
    16801 gaagtggctc caccgaaaga agatccggta ccattaccgg agctgccgtg cgaaaaatca
    16861 gatgcctatt tcgtgctgcg tgacggtgct gccggtgtgt tcctggcggc caataccttc
    16921 ccgaaatcgc gtgaaacgcg tgcgccgctg gtggaagagc tgtatcgctt ccgcgatcgt
    16981 ctgccggaaa aactgcgtta tctggccgat gcgccgcagc aggatccgga aggtaataag
    17041 actatggttc gctttagccg taaaaccaaa cagcaatatg tctcttcgga aaaagacgga
    17101 aaggcgactg gctggtcagc attttatgtt gatggcaaat gggttgaagg gaaaaaataa
    17161 cctttaattc tgtcaggttt ttataaacaa agggtcgcga aagc
//
LOCUS       DBFIFM010000093        17076 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.093, whole genome shotgun sequence.
ACCESSION   DBFIFM010000093 DBFIFM010000000
VERSION     DBFIFM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.093"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(469..1665)
                     /gene="hemY"
                     /locus_tag="N7C74_002623"
     CDS             complement(469..1665)
                     /gene="hemY"
                     /locus_tag="N7C74_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HGU2432467.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1668..2867)
                     /gene="hemX"
                     /locus_tag="N7C74_002624"
     CDS             complement(1668..2867)
                     /gene="hemX"
                     /locus_tag="N7C74_002624"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HGU2432468.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKVELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQQETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPQADTP
                     AAAPQGE"
     gene            complement(2889..3629)
                     /gene="hemD"
                     /locus_tag="N7C74_002625"
     CDS             complement(2889..3629)
                     /gene="hemD"
                     /locus_tag="N7C74_002625"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HGU2432469.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3626..4567)
                     /gene="hemC"
                     /locus_tag="N7C74_002626"
     CDS             complement(3626..4567)
                     /gene="hemC"
                     /locus_tag="N7C74_002626"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HGU2432470.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4954..7500
                     /gene="cyaA"
                     /locus_tag="N7C74_002627"
     CDS             4954..7500
                     /gene="cyaA"
                     /locus_tag="N7C74_002627"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HGU2432471.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7540..7860)
                     /gene="cyaY"
                     /locus_tag="N7C74_002628"
     CDS             complement(7540..7860)
                     /gene="cyaY"
                     /locus_tag="N7C74_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HGU2432472.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFRDLLEQAATQQAGET
                     VSFR"
     gene            complement(8022..8164)
                     /locus_tag="N7C74_002629"
                     /pseudo
     CDS             complement(8022..8164)
                     /locus_tag="N7C74_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            8323..8526
                     /gene="yifL"
                     /locus_tag="N7C74_002630"
     CDS             8323..8526
                     /gene="yifL"
                     /locus_tag="N7C74_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU2432473.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8563..9387
                     /gene="dapF"
                     /locus_tag="N7C74_002631"
     CDS             8563..9387
                     /gene="dapF"
                     /locus_tag="N7C74_002631"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HGU2432474.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9384..10091
                     /gene="yigA"
                     /locus_tag="N7C74_002632"
     CDS             9384..10091
                     /gene="yigA"
                     /locus_tag="N7C74_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HGU2432475.1"
                     /translation="MKQPGEELQETLTELDDRVVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQALANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            10088..10984
                     /gene="xerC"
                     /locus_tag="N7C74_002633"
     CDS             10088..10984
                     /gene="xerC"
                     /locus_tag="N7C74_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HGU2432476.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQNWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKTPRHLPKNIDVDDINRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNALSWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            10984..11700
                     /gene="yigB"
                     /locus_tag="N7C74_002634"
     CDS             10984..11700
                     /gene="yigB"
                     /locus_tag="N7C74_002634"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HGU2432477.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEQEALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            11784..13946
                     /gene="uvrD"
                     /locus_tag="N7C74_002635"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             11784..13946
                     /gene="uvrD"
                     /locus_tag="N7C74_002635"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HGU2432478.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(13991..14893)
                     /locus_tag="N7C74_002636"
     CDS             complement(13991..14893)
                     /locus_tag="N7C74_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HGU2432479.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSIV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSRQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCDK"
     gene            complement(14976..15740)
                     /gene="yigE"
                     /locus_tag="N7C74_002637"
     CDS             complement(14976..15740)
                     /gene="yigE"
                     /locus_tag="N7C74_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HGU2432480.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            15902..15958
                     /gene="ysgD"
                     /locus_tag="N7C74_002638"
     CDS             15902..15958
                     /gene="ysgD"
                     /locus_tag="N7C74_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HGU2432481.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            16110..17060
                     /gene="corA"
                     /locus_tag="N7C74_002639"
     CDS             16110..17060
                     /gene="corA"
                     /locus_tag="N7C74_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HGU2432482.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
BASE COUNT         3883 a         4473 c         4562 g         4158 t
ORIGIN      
        1 gattattgat gctgttttta gttttaacgg caattaatat atgtgttatt aattgaatga
       61 attttatcat tcataataag tatgtgtagg atcaagctca ggttaaatat tcactcagga
      121 agttattact caggaagcaa agaggattac agaattatct cataacaagt gttaagggat
      181 gttatttccc gattctctgt ggcataataa acgagtagat gctcattcca tctcttatgt
      241 tcgccttagt gcctcataaa ctccggaatg acgcagagcc gtttacggtg cttatcgtcc
      301 actgacagat gtcgcttatg cctcatcaga caccatggac acaacgttga gtgaagcacc
      361 cacttgttgt catacagacc tgttttaacg cctgctccgt aataagagca ggcgtttttt
      421 tatgtgtcag gaaggtcccg gaggtgcttg cctccgggtg agaaggaact actgtggtgg
      481 gttgttctgc aacgttaaca tcaaaccgtc gcgacgcatc gctgcagctt cttccggctt
      541 gtgcagtctg tccagcgcgt cggcaagcca tgcgtaatcg taggcgtccg gacgttgttt
      601 cagcgctgcg cggaaggcga gcgatgcttc ctgccattct ccgtgcttca tcagtgactg
      661 gcccagtgtg ctccacaaca gcgggcgatc gccgacgttt ttgatttgct ggcgcagcac
      721 tttttccagc tgttccggat tgtttgtttt cagtcgagga atcggcagca gtaggcgatc
      781 gtcgtactgg cgtttcaggc catcgatgat aatttgctgg gcagtatcat gatcgtcaca
      841 ttcaataaga tgttccgcca ttgccacctg caacgctacc tgatgacgcg ttttccggct
      901 ttggtttttc caccagttac gcaaaccttc gctaccgtta tcggcacgcg cctgatccat
      961 caggccaatc catgcctgtt gttccagcat tgcacgatgt tcttcatcac caacatgggc
     1021 tttcgccatt gatgggataa tatccagcag cgaactccat gcacctgtgc ggatatacgc
     1081 ctgttccgcc agacgtaata cttccggatg gcgtggcgta acttccagca gcttatccac
     1141 gccgtggcgt gcagcatggt tttcattacg ggccagttgc agacgtacac gggtgatttc
     1201 taccggaatg gtgtcattgc cggccagctc cgctgcgcgt tccagatgtt ggttggcgcg
     1261 cgcttcatca ccacgttgtt gcgcggcttc ggcagccagt agatagttca ccaccggttg
     1321 ttcagcgtga tcggcatttt tcgccatcag cttttcaact tgctgataat cgccttccgc
     1381 cagtttcagc agcgcctgtt cggtctgctt acgtgcacgg cgacgcttac gcccgacaaa
     1441 ccacccacgg gtgtgcgcgc cagtgcggaa gatccgccgc agtagccact caatggcaaa
     1501 cagcactacc atcgccagaa tcaatatgat cgccaggccc gtgacgctgg tttcgatatt
     1561 gtagttgtcg gtctggatca gcacataacc ctgatgaccg gcaatcatcg ggccaaccac
     1621 gatccccgca atcagcaaca caaagagcaa taacactttt agcatggtta ttctccttgc
     1681 ggcgcggctg ccggagtatc agcttgcggt gcaggtgcag gtgcaggtgc aggtgcaggt
     1741 ttagcttccg ttgtccccgc tgccggttgt gccagcaggt tacgcacgcg agtctgcatc
     1801 agtttttcca gcatcgcctg gctttgcagg gtttccggaa gatccatcga gatattttgc
     1861 tggcttaact ggtctacctc gtcgaggaac gctttggtgg tggcatcatc agtatcgtag
     1921 taagcacgta cccaggtgga gacgttctcc agcgcctggc gataagtctc ttgctggtga
     1981 cgcggtacag cttgtgctgc gaccagcagg cgagagcgaa tattttcgcg cagatagata
     2041 tcctgatttg gcgctaacag cggtacggcg gtgtcatcac gacggcgaat cgtaatgaag
     2101 ttgtccataa agttctgcca gcttttttgc agattgatac gccattcgct gatggaactg
     2161 gaaagctctt caccgtcgga atccatcggc gaaccatcgc tgtcattatc ggccagacgc
     2221 aggttatcta cctgatttga aagctgatta agcttaagga tgatgccgtc ataatccacc
     2281 tgcgatactg cagaaaggct ggcgatatca tcggtaattg cccgacgaac ggtaatcaga
     2341 ctcgggtcat tcatatccgc caggctggcg tctgcacttt tcagcaacgc tgcagcggtc
     2401 gtgacgtcct gatcgctcca cagcttccgt ccggcgagtt tcaccagaaa atcggcctga
     2461 gccagcagcc aggttttagc atcgctgccg gaaatggtgg cgaccttttg ttggacttca
     2521 tccaactgtt ttgccagcgt ttcttgctga cgattcgcct gcttaagctg tgcagcttgt
     2581 tgcttaataa tgccttccag ctcgactttt tggctctcct gggctttttg caatgccgtc
     2641 agttggttag ccagggcatc gctggtggcg gtctgattga cggcctgttg tttaccccag
     2701 ccatacaaac cgacgcccgc cgccagagca atagcgatag ccaccgcgct gagaatcaat
     2761 gcggtattgt tcttactctt tttttctgtt gcgacaggtt gtgacgtggt gtccacggcc
     2821 tccctggtct cttcaaccac ggcggaggtt ttttcttgtt ccgtcattat ggcttcctgt
     2881 tatgagagtt attgtaatgc ccgtaaaagc gcatcgttgt cagcgttatc ggcgacctta
     2941 atgtcttgcc agcccagttc ccgggcgagt ttcgccaaac gctcactgac gaccaatagt
     3001 cgacagtgta gtaaccagtg ctcacgatac cattgtggga tcagcgacca gagttgctgc
     3061 aacatttcac cgctggtaac aacgaccgtc gtcacctcgc gggattgcca gcgcatcgct
     3121 tcttctgcac catcgtaatg gattgcgcat cgttgataac attcacaaaa agtgacctca
     3181 gcaccgcgcg ccgtcagggt atccccaatt agctcacgac cgccattgcc acgtaatatc
     3241 agcgcacgtt tgcccgcaat attttgtaat tcaggtaatt gtagcaagac ttcgctgatt
     3301 tcccgatcct gcgggtagag aatcttctgt ccgcttacgg tatgtagtgc cagtgcggtg
     3361 gtgcgtccaa tggcgaaata attaggtagt cggggccatt tacgatcttg ctgatgtagc
     3421 tgtgattggg caaaagcaac cgcgtgttgc gagagggcaa acaacagatc gctctccccc
     3481 agcgctgcca gttgatcagc aagttgcggt aattgtcgac ccggagaaaa ctcaatcagc
     3541 ggaaaatgcc aggccacctg ccccagtgtg cgcagacggc tcactaactc ttctccagcg
     3601 ggagacgggc gggtgacaag gatactcatg ccggggcgtc tccgttatag acttcagcga
     3661 ggatctcgcg cgcgccgtta ttcagtagct cttctgccag cgaaatcccc atttgttcgg
     3721 catcttgcgg cgcaccgcgg cgttcaccgc gaataatctg cgaaccgtcc ggcgcgccga
     3781 ccagcgcacg cagccagatt tcgccatcaa taagctcggc gtagctacca attggcacct
     3841 gacatccacc ttcgagacgg gtattcatgg cgcgttctgc ggtaacgcgc agtgcagttt
     3901 cgtggtgatt cagcgcggca agcagctcgc gagtgcgtgt atcatcaagg cggcattcaa
     3961 taccgactgc accttgtcct accgccggaa gagaaatctc gggtggcaac gcggcgcgaa
     4021 tgcgggactc cagacctaaa cgttttagtc cggctacggc aagaatgatg gcatcgtatt
     4081 cgccgttatc cagtttgctc aggcgagtgc cgacgttgcc gcgcagggag cggataatca
     4141 gatccgggcg gcgttcagcc agttggcact ggcgacgtaa actggacgtc ccgacgatac
     4201 tgcctgccgg taacgcatcc agactgtcat agttattgga cacaaaggca tcgcgaggat
     4261 cttcacgctc acaaatagtg accaggccca gaccttgcgg gaattcaacc ggcacatctt
     4321 tcattgaatg tacggcgata tcggcgcgat tttcgaggag cgcgacttcc agctctttta
     4381 caaataagcc ttttccgccg actttcgcca gcggcgtatc aagaatcaca tcgccgcgcg
     4441 tcaccatcgg taccagttca acgaccaggc ccggatggct cgccatcaac ttgtctttga
     4501 catagtgtgc ctgccagagt gcaagtgggc tttggcgtgt ggcaattctt aaaacattgt
     4561 ctaacatgct tgttaccgtc attatcatcc gtggtccatc ctaacatcct tgccagagtg
     4621 atgtcagtgt tgtggtgaaa cgtagacgcc tgcgcaaacc gtaaaatgag gtctggcagt
     4681 ggatcctgac aggcgtttca cgccgtcgta ataaggaatt tacagagaat aaacggtgct
     4741 acacttgtat gtagcgcatc tttctttacg gtcaatcagc aaggtgttaa attgatcacg
     4801 ttttagacca ttttttcgtc gtgaaactaa aaaaaccagg cgcgaaaagt ggtaacggtt
     4861 acctttgaca tacgaaatat cccgaatgcc gcgtgttacc gttgatgttg gcggaatcac
     4921 agtcatgacg ggtagcaaat caggcgatac gtcttgtacc tctatattga gactctgaaa
     4981 cagagactgg atgccataaa tcaattgcgt gtggatcgcg cgcttgctgc tatggggcct
     5041 gcattccaac aggtctacag tctactgccg acattgttgc actatcacca tccgctaatg
     5101 ccgggttacc ttgatggtaa cgttcccaaa ggcatttgcc tttacacgcc tgatgaaact
     5161 caacgccact acctgaacga gcttgaactg tatcgtggaa tgtcagtaca ggacccgccg
     5221 aaaggtgagc ttccaattac tggtgtatac accatgggca gcacctcgtc cgtagggcaa
     5281 agttgttcct ctgacctgga tatctgggtc tgtcatcaat cctggctcga tagcgaagag
     5341 cgccagttgc tacaacgtaa atgcagcctg ctggaaagct gggccgcctc gctgggtgtg
     5401 gaagttagct tcttcctgat tgatgaaaac cgttttcgcc acaacgagag tggcagtctg
     5461 gggggcgaag attgtggctc cacccagcat atactgctgc ttgatgaatt ttatcgtacc
     5521 gccgtgcgtc tcgccggtaa gcgtattctg tggaatatgg tgccgtgcga cgaagaagag
     5581 cattacgatg actacgtaat gacgctctac gcacaaggcg tgctgacgcc aaatgaatgg
     5641 ctggatctcg gcggcttaag ctcgctttcc gctgaagaat acttcggtgc cagcctttgg
     5701 caactctaca agagtatcga ttctccatac aaagcggtgc tgaagacact gctgctggaa
     5761 gcctattcct gggaataccc gaacccacgc ctgctggcga aagatatcaa acagcgtttg
     5821 cacgacggcg agattgtatc gtttggcctc gatccttact gcatgatgct ggagcgtgtt
     5881 actgaatacc tgacggcgat tgaagatttc acccgtctgg atttagtacg tcgctgcttc
     5941 tatttaaaag tgtgcgaaaa gctcagccgt gaacgcgcct gcgtaggctg gcgtcgcgca
     6001 gtgttgagcc agttagtgag cgagtggggg tgggacgaag ctcgtctggc aatgctcgat
     6061 aaccgcgcta actggaagat tgatcaggtg cgtgaggcgc acaacgagtt gctcgacgcg
     6121 atgatgcaga gctaccgtaa tctgatccgc tttgcgcgtc gcaataacct tagcgtctcc
     6181 gccagtccgc aggatatcgg cgtgctgacg cgtaagctgt atgccgcgtt tgaagcatta
     6241 ccaggtaaag tgacgctggt aaacccgcag atttcacccg atctctcgga accgaatctg
     6301 acctttattt atgtgccgcc gggccgggct aaccgttcag gttggtatct gtataaccgc
     6361 gcgccaaata ttgagtcgat catcagccat cagccgctgg aatataaccg ttacctgaat
     6421 aaactggtgg cgtgggcatg gtttaacggc ctgctgacct cgcgcacccg cttgtatatt
     6481 aaaggtaacg gcattgtcga tttgcctaag ttgcaggaga tggtcgccga cgtgtcgcac
     6541 catttcccgt tgcgcttacc tgcaccgaca ccgaaggcgc tctacagccc gtgtgagatc
     6601 cgccatctgg cgattatcgt taacctggaa tatgacccga cagctgcgtt ccgcaatcag
     6661 gtggtgcatt ttgatttccg taagctggat gttttcagct ttggcgagaa tcaaaattgc
     6721 ctggtaggta gcgttgacct gctgtatcgc aactcgtgga atgaagtgcg tacgctgcac
     6781 ttcaacggcg agcaatcgat gatcgaagcc ctgaaaacta ttctcggcaa aatgcatcag
     6841 gacgccgcac cgccagatag cgtggaagtc ttctgttata gccagcatct gcgcggctta
     6901 attcgtactc gcgtgcagca actggtttct gagtgtattg aattgcgtct ttccagcacc
     6961 cgccaggaaa ccgggcgttt caaggcgctg cgcgtttctg gtcaaacctg gggcttgttc
     7021 ttcgaacgcc tgaatgtatc ggtacagaaa ctggagaacg ccatcgagtt ttatggcgct
     7081 atttcgcata acaaactgca cggcctgtca gtgcaggttg aaaccaatca cgtcaaatta
     7141 ccggcggtgg tggacggctt tgccagcgaa gggatcatcc agttcttttt cgaagaaacg
     7201 caagatgaga atggctttaa tatctacatt ctcgacgaaa gcaatcgggt tgaggtgtat
     7261 caccactgcg aaggcagcaa agaggagctg gtacgtgacg tcagtcgctt ctactcgtca
     7321 tcgcatgacc gttttaccta cggctcaagc ttcatcaact tcaacctgcc gcagttctat
     7381 cagattgtga aggttgatgg tcgtgaacag gtgattccgt tccgcacaaa atctatcggt
     7441 aacatgccgc ctgccaatca ggatcacgat acgccgctat tacagcagta tttttcgtga
     7501 tgaacgtgcc ggaaagcgag gcttatccgg catgcaatct tagcggaaac tgactgtttc
     7561 acccgcctgc tgcgtcgccg cctgttccag caaatcccgg aaggtttcgc cgctgcgatc
     7621 gcaaatccac tcatcgcctt tcagatcaaa atggtagccg ccttgtttgg ttgccagcca
     7681 tacctggtgc agcggttcct ggcggttgat aatgattttg ctgccattct caaaggtaat
     7741 ggtcagcacg ccgccgttga tttcgcagtc gatatcgctg tcgccatccc agtcgtccag
     7801 acgctcttca atggtcagcc acagttgatc agccaggcga tgaaattcac tgtcgttcat
     7861 tgttgtatcc tgtttttaag tgatggcggc agtatagcgg catggggtca gggcttcaaa
     7921 gtttgcacct ctgcggctgc gttacggcat gattcatccg tcaccggaat aatgatgtct
     7981 ctgtgtagcg aaagatttgt ctcttcatta gggcgcagtt acaccacgcc tttccctgtt
     8041 tctggtaaac attatgatca ggtttaccga gcgagcatcc tcacgctgac ggaactaaaa
     8101 aagccaacaa acaaaccaca ttgcgatagt gcataaagcc atcctggcgt gaggtgttga
     8161 tcacgaaact gccagcagaa cataaatcct cacgagtaag cgttatactc gcagcatttc
     8221 ctcacttttc agacttcata aagagtcgct aaacgcttgc ttttacgcct tctcctgcga
     8281 tgatagaaag cagaaagcga tgaactttac aggcaatcca taatgaaaaa cgtgtttaag
     8341 gcactcactg tattacttac tctcttcagc ctgacgggct gcggtctgaa aggtccgctc
     8401 tatttcccgc ctgcagataa aaatgcaccg ccgccgacca aaccggtaga gacgcaaacg
     8461 caatccacgg tgccggataa aaacgatcgc gccactggcg atggtccatc ccaggtgaat
     8521 tactaaaagt cagtttctgt acccgcgtga ttggagtaaa tgatgcagtt ctcgaaaatg
     8581 catggccttg gcaacgattt tatggtcgtc gacgcggtaa cgcagaatgt ctttttttca
     8641 ccggagctga ttcgtcgcct agctgatcgg cacctggggg tagggtttga ccaactgctg
     8701 gtggttgagc cgccgtatga tcctgaactg gattttcact atcgcatttt caatgctgat
     8761 ggcagtgaag tggcgcagtg cggcaacggc gcacgctgct ttgcccgttt tgtgcgtctg
     8821 aaagggctga ccaataagcg cgatatccgc gtcagcaccg ccaacgggcg gatggttctt
     8881 accgtcaccg atgatgatct ggtccgcgta aatatgggcg aacccaactt cgaaccttct
     8941 gccgtgccgt ttcgcgctaa caaagcggaa aagacctata ttatgcgcgc cgccgagcag
     9001 acaatcttat gcggcgtggt gtcgatggga aatccacatt gcgtgattca ggtcgatgat
     9061 gtcgatactg cggcggtaga aacactgggg cctgttctgg aaagccacga gcgttttccg
     9121 gagcgcgcca atatcggttt tatgcaagtg gttaagcgcg agcatattcg tttacgcgtt
     9181 tatgagcgtg gggcaggaga aacccaggcc tgcggcagcg gcgcatgtgc ggcggttgca
     9241 gtagggattc agcaaggttt gctggccgaa gaagtacgcg tggaactccc aggcggtcgt
     9301 cttgatatcg cctggaaagg tccgggtcac ccgttatata tgactggccc ggcggtacat
     9361 gtctacgacg gatttattca tctatgaagc aaccagggga agaactgcag gaaacactca
     9421 cggagcttga tgaccgggtg gttgtcgatt atctgattaa aaatcctgag ttttttatcc
     9481 gcaatgcgcg cgcagtagaa gcgatacgtg tgccgcatcc ggtacgcggt accgtttcgt
     9541 tggtcgagtg gcacatggcc cgcgcacgta atcatattca tgttctggaa gagaacatgg
     9601 cgctgttgat ggaacaggct ctcgccaacg aaggcctgtt ttatcgctta ctctacctgc
     9661 agcgcagtct caccgccgcc agcagtcttg atgatatgct gatgcgcttt caccgctggg
     9721 cgcgtgatct cggcctggca ggtgcgagtc tgcgcctgtt tccggatcgc tggcgcttag
     9781 gtgcgccgtc gaaccacact catctggcat taagccgtca gtctttcgaa ccgctgcgta
     9841 ttcagcgtct ggggcaggag cagcattatc tggggccgct taacggaccg gagctgctgg
     9901 tggtactacc ggaagcgaaa gcggtgggat cggtggcgat gtcgatgctg ggaagcgatg
     9961 ctgatttagg cgtggtgctg ttcaccagcc gcgatgccag tcactatcaa caagggcagg
    10021 gaacgcagtt acttcatgaa attgcgctga tgttgccgga gcttctggag cgttggattg
    10081 aacgcgtatg accgatttac acaccgatgt agaacgctac ctacgttatc tgagcgtgga
    10141 gcggcagctt agtccgataa ctctgcttaa ctaccagcga cagcttgagg cgatcatcaa
    10201 ttttgccagc gaaaacggcc tgcaaaactg gcagcaatgc gatgcagcga tggtgcgcaa
    10261 ttttgctgta cgcagtcgcc gtaaagggct gggcgcggca agcctcgcat tacgcctctc
    10321 tgcgctacgt agcttttttg actggctggt cagccagaac gaactcaaag ctaacccggc
    10381 gaaaggtgtt tcggcaccga aaacgccacg tcatctgccg aaaaatatcg acgtcgatga
    10441 tataaatcgc ctgttggata ttgatatcaa cgatcccctc gctgtgcgcg accgtgcaat
    10501 gctggaagtg atgtacggcg cgggcctgcg tctttccgag ctggtggggc tggacatcaa
    10561 acacctcgac ctggagtccg gcgaagtgtg ggtgatgggg aaaggcagca aagagcgccg
    10621 cctgccgatt ggtcgtaacg ctttgtcatg gattgagcac tggcttgatt tgcgcgacct
    10681 gtttggtagc gaagacgacg cgctttttct gtcgaaactg ggcaagcgta tctccgcgcg
    10741 taatgtgcag aaacgctttg ccgaatgggg cataaaacaa gggctgaata atcacgttca
    10801 cccgcataaa ttacgtcact cgttcgctac gcatatgctg gagtcgagcg gcgatcttcg
    10861 tggtgtgcag gagctgctgg gtcatgccaa cctctccacc acgcaaatct atactcatct
    10921 tgattttcaa caccttgcct cggtgtacga tgcggcgcat ccacgcgcca aacgggggaa
    10981 ataatgcgtt tttaccggcc tttggggcgc atctcggcgc tcacctttga cctggatgat
    11041 accctttacg ataaccgtcc ggtgattttg cgcaccgagc aggaggcgct tacctttgtg
    11101 caaaattacc atccggcgct gcgcagcttc cagaatgaag atctgcaacg cctgcgccag
    11161 gcggtacggg aagcggaacc cgagatttat cacgacgtga cgcgctggcg ttttcgctca
    11221 atagaacaag cgatgcttga cgccggattg agcgcggaag aagccagtgc aggcgcacac
    11281 gcggcaatga tcaactttgc taaatggcgc agcaggatcg acgtcccgca gcaaacccac
    11341 gacaccttaa aacagctggc gaagaaatgg ccgctggtgg cgatcaccaa cggtaacgct
    11401 cagccggaac tgtttggttt gggggattat tttgagtttg tgctgcgcgc tggcccgcac
    11461 gggcgctcga aaccgttcag cgatatgtac ttcctggcgg cggaaaaact caacgtgccg
    11521 atcggcgaga tcttacatgt aggtgacgat ctcaccactg acgtgggtgg ggcaattcgc
    11581 agcggaatgc aggcctgttg gatcagaccg gaaaatggcg atctgatgca aacctgggat
    11641 agccgtttac tcccgcatct ggaaatttcc cggttggcat ctctgacctc gctgatataa
    11701 tcagcaaatc tgtatatata cccagctttt tggcggaggg cgttgcgctt ctccgcccaa
    11761 cctattttta cgcggcggtg ccaatggacg tttcttacct gctcgacagc cttaatgaca
    11821 aacagcgcga agcggtggcc gcgccgcgca gcaaccttct ggtgctggcg ggcgcgggca
    11881 gtggtaagac gcgcgtactg gtgcatcgta tcgcctggct gatgagcgtg gaaaactgct
    11941 cgccatactc gattatggct gtgacgttta ccaacaaagc ggcggcggag atgcgtcatc
    12001 gtatcgggca actgatgggc accagccagg gcggcatgtg ggtcggcacc ttccacgggc
    12061 tggcgcaccg tttgctgcgt gcgcaccata tggacgccaa tctgccgcag gatttccaga
    12121 tcctcgatag cgaagaccag ctacgcctgc ttaagcgtct gatcaaagcc atgaacctcg
    12181 acgagaagca gtggccgccg cgccaggcaa tgtggtacat caacagccag aaagatgaag
    12241 gcctgcgtcc gcatcatatt caaagctacg gtaatccggt ggagcagacc tggcagaagg
    12301 tgtatcaggc gtatcaggaa gcgtgcgatc gtgcaggcct ggtggacttc gccgagctgc
    12361 tgctgcgcgc tcacgagttg tggcttaaca agccgcatat cctgcaacac taccgcgaac
    12421 gttttaccaa catcctggtg gacgaattcc aggataccaa caacattcag tacgcgtgga
    12481 tccgcctgct ggcgggcgac accggcaaag tgatgatcgt cggtgatgac gaccagtcaa
    12541 tctacggctg gcgcggggcg caggtggaga atattcagcg tttcctcaat gatttccccg
    12601 gtgccgaaac tatccgtctg gagcagaact atcgttctac cagtaacatt ctgagcgccg
    12661 cgaacgccct gattgaaaac aacaacgggc gtctgggtaa aaaactgtgg accgatggcg
    12721 cggacggtga gcctatttcc ctctattgcg cttttaacga actcgatgaa gcgcgttttg
    12781 tcgttaaccg catcaaaacc tggcaggaca acggtggggc gctcgccgag tgcgccattc
    12841 tctaccgcag caacgcccag tcgcgggtgc tcgaagaggc gttattgcag gccagtatgc
    12901 cgtaccgtat ttacggcggg atgcgcttct tcgaacgcca ggaaatcaaa gatgcgctct
    12961 cgtatctgcg cctgattgcc aaccgcaacg acgacgcggc ctttgagcgt gtggtgaata
    13021 cgccaacgcg gggtattggt gaccggacgc tggacgtggt acgtcagaca tcgcgcgatc
    13081 gccagttaac actctggcag gcatgtcgtg aactgttgca ggaaaaagcc ctcgccggac
    13141 gtgctgccag cgccttacag cggtttatgg aactgatcga cgccttagcg caggaaactg
    13201 ccgatatgcc gctgcatgta cagactgacc gggtaattaa agactccggc ctgcgtacca
    13261 tgtacgagca ggagaagggc gaaaaaggtc agacgcgtat cgaaaactta gaggaactag
    13321 tgacggcaac gcgccaattc agctacaacg aagaagacga agatttaatg ccgctgcagg
    13381 cgttcctctc ccatgcggcg ctggaagcgg gcgaggggca ggcggatacc tggcaggatg
    13441 cagtgcagtt gatgacgctg cactcggcga aaggcctgga gttcccgcag gtgtttatcg
    13501 tcggtatgga agagggcatg ttcccaagcc agatgtcgct ggatgaaggc ggacgtctgg
    13561 aagaagaacg ccgtctggcc tacgttggtg taacccgcgc gatgcagaaa ctgacgctga
    13621 cctacgcgga aactcgccgt ctgtatggca aagaggttta ccatcgcccg tcgcgcttta
    13681 tcggtgagtt gccggaagag tgtgtggaag aggtgcgcct gcgcgccacg gtaagccgcc
    13741 cggtcagcca tcagcgtatg ggtacgccga tggtcgagaa cgacagcggc tacaagctcg
    13801 gccagcgtgt acgccacgct aagtttggtg aaggcactat cgtcaatatg gaaggcagcg
    13861 gcgagcatag ccgtttgcag gtggcattcc agggccaggg tattaaatgg ctggtggcgg
    13921 catacgcccg gctggagacg gtataacgtt gcgggatgcg gtgcgccaca ccgcatccgg
    13981 catcagcacc ttacttatca catttcttca catcaatatt ccagtcagca ccagacgcaa
    14041 cctgatgttt ctgcgcgaaa ttatccatat tcagcgccac ggaaacattc aataagctgt
    14101 tcagatacac catcggctgt ccttccggca catcgccgaa gcttgcgaca tacggcattt
    14161 tccctgcgta tttttgccgg gaaccttcgg aaatggtcac acacaacgtg tcgttgagtt
    14221 tgatccccgc ctgattcagc aactcgtcgc tgatattgct ccagacgttg ccgtactgaa
    14281 tatcgagaat cgggatattg cccttcactt caccttttgt tgcttttgct ttctgataag
    14341 aaagttcgac aacctttgca ggaagctcag gccccacctg ctcgaacgtg atcgccccgg
    14401 aagccagacg tgcaccggtg taggcataca cgtcacgacc gtggaaggta tatgactttt
    14461 cagaaccttt caggcggtta gctttttcgt cgatttcccg gacgctctca atacccagcg
    14521 attccgccac caaagtgagc gtaccgttat ccggtgaaac gaagtattga ccgtttttgg
    14581 ttttcagcac tatcgattta cggtcagtgc ctacgcccgg atcgaccaca ctgacaaata
    14641 ccgaaccttg cggccagtaa ctggcggtct gatacaggcg gtaagcgcct tcccagatgt
    14701 tatacggtgg aatttcgtgt gttaaatcaa atattttaag attatggtca acaccgaatg
    14761 cgaccccttt catcgccgag acggcaccat ctttcaggct aaaatcagtt tgtaaaatca
    14821 aagcgttatc cgcataagcg gatgatgatc ccagacacgt taataaaaat aacgttgcga
    14881 gcgttttttt catttttgtt tcccttcatt tatctgatga ttttccagct gtgtagccag
    14941 ccccagtttg tctggcctcc tcgagtgtac aatgcttaac ctttccgctc gacggaaatc
    15001 atggtgacaa aaggatagcg ttgccacggg attgccccgc ctttcatata catatgtgaa
    15061 atcgtgccat caagataaag cagctgctca acgttcagtt tcgctttggc ataacaggca
    15121 aaatcataaa aatttgtcgc ctgctggctc aacaaaaaca cggcattccc atgtttatta
    15181 attccaacac cgttacgaat tttacgtgag gcgacgttgg gatgaatacg cggattaatt
    15241 acactgtttt ccatcaacat tggccctgac tgcaccgcaa actgaatctc tttactggtt
    15301 ttgaaggcat ccagacgaac gatgccgact ttatctcccg cgacataaaa cacgccgcca
    15361 ggacggataa agaaattccc ttcacctgaa gcgagattta acgccacctt ctgctgaccg
    15421 ttttcgatgt acaaaccgag cggcgcatag ctttcatcat agatgccgcc gttcatcgcc
    15481 atctgcacct gaccctgact attcatatcc gccagcagag catgtaaagt tccccacgct
    15541 tcgccattgg ctttttgcca gtacattttc acccgctctg tttgaggatt aacggtatac
    15601 gcctgtacgg tcagcgttgg atctgagagt gcgcaatcat cagcggcaac agcaaacaag
    15661 ggaagtaagg tgagggcgag aaaaatccgt ttgagattca aggtgatcat tcctttacca
    15721 atgagtagcc gatgcgccat tataggtcct ggatgtggga tttttttatc ctgttagcga
    15781 ccttgacgag taccaaaaag cgcgaagttc aactattgtt ctgtggtgtt ctgttgcgtg
    15841 ttgacggcaa aattttgctg gcgtaacatg cgcgcacgat cactctaaga ggacattcgc
    15901 cttggacaca cccagtagat actggctcac tatcctgtca tccaggatca actcctaagg
    15961 ctatcccttt ttgctgatag ccttagcggt tgtcagcgac ctcaattttt cccgtcgcgc
    16021 tgagtcaggc tgtttaatgg tctgaaaccc aatttgtttc tgtgtgccca ccgaactgtc
    16081 cgatatttta agcattggga gtcccggtca tgctgagcgc atttcaactg gaaaataacc
    16141 gactgacccg gctggaagtc gaagagtcac aaccccttgt aaatgcagta tggattgatc
    16201 ttgtcgaacc ggacgacgac gagcgactgc gcgtacaatc tgaacttggc cagagcctgg
    16261 caacccgccc ggaactggaa gacatcgaag catcggcacg tttctttgaa gacgatgacg
    16321 gcctgcatat tcactccttc ttcttctttg aagatgcgga agatcacgcc ggtaactcca
    16381 ctgtggcatt taccatccgt gatggtcgtc tgtttactct gcgtgagcgt gaactgcccg
    16441 cttttcgtct gtatcgtatg cgtgcccgta gccagtcgat ggtagacggt aacgcctacg
    16501 agttgctgct ggatctgttc gaaaccaaaa tcgaacagtt ggcagatgaa attgaaaata
    16561 tctatagcga cctggagcaa ttgagccggg tgattatgga agggcatcag ggcgatgagt
    16621 acgacgaggc gctctccact ctggcggaac tggaagatat cggctggaaa gtgcgcctgt
    16681 gtctgatgga tacccagcgc gcgctcaact tcctggtacg taaagcacgc ttaccgggtg
    16741 ggcaactgga gcaggcgcgt gaaatcctgc gagatatcga atccctgctg ccgcataacg
    16801 aatccctgtt ccagaaggtg aacttcctga tgcaggcggc aatgggtttt atcaacatcg
    16861 agcagaaccg catcatcaaa attttctcgg tggtatccgt cgtgttcctg ccgccaacgc
    16921 tggttgcttc cagctacggg atgaactttg agtttatgcc agaactgaag tggagtttcg
    16981 gctacccagg cgcgattatc tttatgatcc tcgcgggcct ggcaccgtat ctgtacttta
    17041 agcgcaagaa ctggctgtaa ctatacgatg tcgctg
//
LOCUS       DBFIFM010000094        17034 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.094, whole genome shotgun sequence.
ACCESSION   DBFIFM010000094 DBFIFM010000000
VERSION     DBFIFM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.094"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..242
                     /locus_tag="N7C74_002640"
     CDS             <1..242
                     /locus_tag="N7C74_002640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432483.1"
                     /translation="LPSFIMISVFTTSPFRQDRPKFHAYTICVLAIDPFLTLRVVFPA
                     YRNTFVVRKVCKGKRLPCDFAGAEVRVWSEMEWQQ"
     gene            230..1255
                     /gene="purR"
                     /locus_tag="N7C74_002641"
     CDS             230..1255
                     /gene="purR"
                     /locus_tag="N7C74_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HGU2432484.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(1252..2184)
                     /gene="punR"
                     /locus_tag="N7C74_002642"
     CDS             complement(1252..2184)
                     /gene="punR"
                     /locus_tag="N7C74_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HGU2432485.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            2297..3508
                     /gene="punC"
                     /locus_tag="N7C74_002643"
     CDS             2297..3508
                     /gene="punC"
                     /locus_tag="N7C74_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HGU2432486.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAAMLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            3799..4947
                     /gene="cfa"
                     /locus_tag="N7C74_002644"
     CDS             3799..4947
                     /gene="cfa"
                     /locus_tag="N7C74_002644"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HGU2432487.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFTVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFVAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(4987..5628)
                     /gene="ribE"
                     /locus_tag="N7C74_002645"
     CDS             complement(4987..5628)
                     /gene="ribE"
                     /locus_tag="N7C74_002645"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HGU2432488.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQSGTEA"
     gene            5843..7216
                     /gene="mdtK"
                     /locus_tag="N7C74_002646"
     CDS             5843..7216
                     /gene="mdtK"
                     /locus_tag="N7C74_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HGU2432489.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRYLQRLPSAI
                     ILQRASR"
     gene            complement(7257..8513)
                     /locus_tag="N7C74_002647"
     CDS             complement(7257..8513)
                     /locus_tag="N7C74_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HGU2432490.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKANDTLLADGTMNNLGGEDSDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTLTVQQGGVLILDGSTVKGDSVTFSIGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVQITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            8821..8897
                     /locus_tag="N7C74_002648"
     tRNA            8821..8897
                     /locus_tag="N7C74_002648"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8855..8857,aa:Val,seq:gac)
     gene            8902..8978
                     /locus_tag="N7C74_002649"
     tRNA            8902..8978
                     /locus_tag="N7C74_002649"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8936..8938,aa:Val,seq:gac)
     gene            9086..9391
                     /locus_tag="N7C74_002650"
     CDS             9086..9391
                     /locus_tag="N7C74_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HGU2432491.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTAQLKKLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            9517..11121
                     /locus_tag="N7C74_002651"
     CDS             9517..11121
                     /locus_tag="N7C74_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HGU2432492.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTLLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARKQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDQFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(11133..11945)
                     /gene="ydhT"
                     /locus_tag="N7C74_002652"
     CDS             complement(11133..11945)
                     /gene="ydhT"
                     /locus_tag="N7C74_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HGU2432493.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGKRCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(11949..12734)
                     /gene="phsC"
                     /locus_tag="N7C74_002653"
     CDS             complement(11949..12734)
                     /gene="phsC"
                     /locus_tag="N7C74_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HGU2432494.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGERIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINGVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(12731..13399)
                     /locus_tag="N7C74_002654"
     CDS             complement(12731..13399)
                     /locus_tag="N7C74_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310405.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGU2432495.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICACACRKTNHAPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(13463..14101)
                     /locus_tag="N7C74_002655"
     CDS             complement(13463..14101)
                     /locus_tag="N7C74_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HGU2432496.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSVAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(14114..16216)
                     /locus_tag="N7C74_002656"
     CDS             complement(14114..16216)
                     /locus_tag="N7C74_002656"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HGU2432497.1"
                     /translation="MTNGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAECWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(16237..16863)
                     /locus_tag="N7C74_002657"
     CDS             complement(16237..16863)
                     /locus_tag="N7C74_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HGU2432498.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         4119 a         4428 c         4022 g         4465 t
ORIGIN      
        1 atctcccgtc atttataatg ataagtgttt ttaccacttc cccttttcgt caagatcggc
       61 caaaattcca cgcttacact atttgcgtac tggccattga ccccttcctg acgctccgtg
      121 tcgtttttcc ggcgtaccgc aacacttttg ttgtgcgtaa ggtgtgtaaa ggcaaacgtt
      181 taccttgcga ttttgcagga gctgaagtta gggtctggag tgaaatggaa tggcaacaat
      241 aaaagatgta gcgaaacgag caaacgtttc cactacaact gtgtcacacg tgatcaacaa
      301 aacacgtttc gtcgctgaag aaacgcgcaa cgccgtgtgg gcagcgatca aagaattaca
      361 ctactcccct agcgcggtgg cgcgtagcct gaaggttaac cacaccaagt ctatcggttt
      421 gctagcgacc agcagcgaag cggcctattt tgccgagatc attgaagcag ttgaaaaaaa
      481 ttgcttccag aaaggttaca ccctgattct gggtaatgcg tggaacaatc ttgagaaaca
      541 gcgggcttat ctgtcgatga tggcgcaaaa acgcgtcgat ggtctgctgg tgatgtgttc
      601 tgagtaccca gagccgttgc tggcgatgct ggaagagtat cgccatatcc caatggtggt
      661 gatggactgg ggtgaagcaa aagctgactt caccgatgcg gtcattgata acgcgttcga
      721 aggcggctac atggccgggc gttatctgat tgaacgcggt caccgcgaaa tcggcgttat
      781 ccccggcccg ctggaacgta acactggcgc aggccgcctt gccggtttta tgaaggcgat
      841 ggaagaagca atgatcaagg tgccggaaag ctggattgtg cagggtgact ttgaacctga
      901 atccggttat cgcgccatgc agcaaatcct gtcgcagccg catcgcccta ctgccgtctt
      961 ctgtggtggc gatatcatgg caatgggcgc actttgtgct gctgatgaaa tggggctgcg
     1021 cgtcccgcag gatgtttcgc tgatcggtta tgataacgtg cgcaacgccc gctattttac
     1081 gccggcgctg acaacaatcc atcagccaaa agattcgttg ggtgaaacag cgttcaacat
     1141 gttgttggat cgtatagtca acaaacgtga agaaccgcag tccattgaag tgcatccgcg
     1201 cttgattgaa cgccgctccg tggctgacgg cccgttccgc gactatcgtc gttaatcacc
     1261 cgttgcggga gtctcttccg gctcccgcag ccactcctta ttcagcgtct cactatcgcc
     1321 gagatactca agcaaccagg ttaacgcagg cgacatatca ttttgctgcc atgtcagaca
     1381 acatgccgaa tccggaaagg ggttttccag ttctaatgct acccacttcc cctcattaag
     1441 ccacggtttg gcgaaatgtg ttggcaccat ccctatgcat aatcctgccg agatacaggt
     1501 tgccgatgat tcccagtcag gcacgacaac tcttttttgg ttatccagca accaggtaat
     1561 acgcttaggt agcgttcgcg aggtgtcttc gcgcaccaac gacggccagt tgcgcaacgt
     1621 atcatcgctg aacgggccat ccatcaacgc cagcgggtga tggctggcaa caacgcaact
     1681 ccagcttagc atccccatat cccggaaggc ataacgaccg cctaccggaa tcgcgcgtgt
     1741 tgcgccaatc gccagttcca cgcgcccgtc ggaaagcgca tcccagacac cgttgaacac
     1801 ttcctgaaag acaagaagtt cgacatcatc aaaatggcga taaaaatcaa cgatcatctg
     1861 ccgtgtacgt tctggcctga caatattatc cactgcgata gctaactgac cgcgccagcc
     1921 gttcgctatc tgctgacatt gctggcgggt gatctgcatt tttttgacaa cagagcgccc
     1981 ttctttgaga aaccacgctc cggcagcggt cagctccaca tcacggtgcc gtcgttcaaa
     2041 cagcggcacc gccagccact cttccagctg acgcacggta tagctgaccg cagaaggaac
     2101 gcgatgcagc tcctgtgccg cagcgctaaa actaccatta cgcgctaccg catcaacaac
     2161 ttcgagtgaa tattctgacc acatagtctg cctgcaaaat ttttgaaacc agtcatcaaa
     2221 tattaccgtt tcacaacact aatttcactc cctacacttt gcggcggtgt ttaattgaga
     2281 gatttagaga atatacatgc aacctgggaa aagattttta gtctggctgg cgggtttgag
     2341 cgtactcggt tttctggcaa ccgatatgta tctgcctgct ttcgccgcca tacaggccga
     2401 cctgcaaacg cctgcgtctg ctgtcagtgc cagccttagt ctgttccttg ccggttttgc
     2461 cgcagcccag cttctgtggg ggccgctctc cgatcgttat ggtcgtaaac cggtattatt
     2521 aatcggcctg acaatttttg cgttaggtag tctggggatg ctgtgggtag aaaacgccgc
     2581 tatgctgctg gtattgcgtt ttgtacaggc tgtgggtgtc tgcgccgcgg cggttatctg
     2641 gcaagcgtta gtgacggatt attatccttc acagaaagtt aaccgaattt ttgcgaccat
     2701 catgccgctg gtgggtctat ctccggccct ggctcctctg ttaggaagct ggctgctggt
     2761 ccatttttcc tggcaggcga ttttcgccac cctttttgcc attaccgtgg tgctgattct
     2821 gcctattttc tggctcaaac ccacgacgaa ggcccgtaac aatagtcagg atggtctgac
     2881 ctttaccgac ctgctacgtt ctaaaaccta tcgcggcaat gtgctgatat acgcggcctg
     2941 ttcagccagt ttttttgcat ggctgaccgg ttcaccgttc atccttagtg aaatgggcta
     3001 cagcccggca gttattggtt taagttatgt cccgcaaact atcgcgtttc tgattggtgg
     3061 ttatggctgt cgcgccgcgc tgcagaaatg gcaaggcaag cagttattac cgtggttgct
     3121 ggtgctgttt gctgtcagcg tcattgcgac ctgggctgcg ggcttcatta gccatgtgtc
     3181 gctggtcgaa atcctgatcc cattctgtgt gatggcgatt gctaatggcg cgatctaccc
     3241 tattgttgtc gcccaggctc tgcgtccctt cccacacgca actggtcgcg ccgcagcgtt
     3301 gcagaacact ctgcaactgg gtctgtgctt cctcgcaagt ctggtagttt cctggctgat
     3361 cagtatcagc acgccattgc tcaccaccac cagcgtgatg ttatcaacag tagtgctggt
     3421 cgcgctgggt tacatgatgc aacgttgtga agaagttggc tgccagaatc atggcaatgc
     3481 cgaagtcgct catagcgaat cacactgatc tatatcgata tacttatact taggctgcta
     3541 acaaaatttt gttgtatgat tgaaattagc ggcctatact aatttcgagt tgttaaagct
     3601 acgataaata ttatgttttt acggggacag gatcgttccc gactcactat ggatggtcat
     3661 ttcggcaagg gttcctcctt tccctctgtt ctacgtcgga ttatagactc gcggtttttt
     3721 ctgcgagatt tctcacaaag cccaaaaagc gtctacgctg ttttaaggtt ctgatcaccg
     3781 accagtgatg gagaaactat gagttcatcg tgtatagaag aagtcagtgt accggatgac
     3841 aactggtacc gtatcgccaa cgaattactt agccgtgccg gtatagccat taacggttct
     3901 gccccggcgg atattcgtgt gaaaaacccc gattttttta aacgcgttct gcaagaaggc
     3961 tctttggggt taggcgaaag ttatatggat ggctggtggg aatgtgaccg actggatatg
     4021 ttttttagca aagtcttacg cgcaggtctc gagaaccaac tcccccatca tttcaaagac
     4081 acgctgcgta ttgccggcgc ccgtctcttc aatctgcaga gtaaaaaacg tgcctggata
     4141 gtcggcaaag agcattacga tttgggtaat gacttgttca gccgcatgct tgatcccttc
     4201 atgcaatatt cctgcgctta ctggaaagat gccgataatc tggaatctgc ccagcaggcg
     4261 aagctcaaaa tgatttgtga aaaattgcag ttaaaacctg ggatgcgcgt actggatatt
     4321 ggctgcggct ggggcggact ggcacactac atggcatcta attatgacgt aagcgtggtg
     4381 ggcgtcacca tttctgccga acagcaaaaa atggctcagg aacgctgtga aggcctggat
     4441 gtcaccattt tgctgcaaga ttatcgtgac ctgaacgacc agtttgatcg tattgtttct
     4501 gtggggatgt tcgagcacgt cggaccgaaa aattacgata cctattttac ggtggtggat
     4561 cgtaatttga aaccggaagg catattcctg ctccatacta tcggttcgaa aaaaaccgat
     4621 ctgaatgttg atccctggat taataaatat attttcccga acggttgcct gccctctgta
     4681 cgccagattg ctcagtccag cgaaccccac tttgtgatgg aagactggca taacttcggt
     4741 gctgattacg atactacgtt gatggcgtgg tatgaacgat tcgtcgccgc atggccagaa
     4801 attgcggata actacagtga acgctttaaa cggatgttta cctattatct gaatgcctgc
     4861 gcaggtgctt tccgcgcccg tgatattcag ctctggcagg tggtgttctc acgcggtgtt
     4921 gaaaacggcc ttcgagtcgc tcgctaaagg ctattctatc gccccctctc cgggggcgat
     4981 ttcagatcag gcttctgtac ctgattgatt catggcattt tctcgtgccg ccagcacacg
     5041 ttctaccgta tctaccactg cctgagtttg tggatcgatt tcaatgttga cgcgtgcgcc
     5101 aagttttttc ttcccaagag tcgtgcgttc cagagtttcc ggaattaaat ggacgcaaaa
     5161 acgcgttggc gtgacttcgc cgacggtcag gctaataccg tcgatgccaa taaatccttt
     5221 gtacaggata tatttcatca actgactatc ctggacttta aaccagatct ggcgattatt
     5281 ttccgaggtt aatattttcg ccacttcagc ggtggtcata atatgacctg acattaagtg
     5341 tccgccaatt tcatcactga atttcgccgc acgctcaacg tttacccaat cccccacttt
     5401 taaatcgcca agattggtaa tgcgcaacgt ttctttcatc aggtcaaaac tgacatggtt
     5461 gccgttaatt tccgtcacgg tcaggcagca accgttatgc gccacggagg caccggtttc
     5521 cagaccgtcc agcatgtggt cgggtaactc caccacatgt gtgcgaaaat ttggtttctc
     5581 gtcaatcgac accagttttg cggtgccctg tacaatcccc gtaaacatac ttacaactcc
     5641 tgaaatcagt taagacattc tgttcagcac aatagcaggt ggaaaacgcc cttaccagtg
     5701 aaggggtaag aatggctatt ttttcactgg agaattaata aatcctcgct acaatagact
     5761 gaatttcccc tgcttcttct ttttgctgcc tattcaggcg gctttttagt ctctcatata
     5821 actacaaata aaaggtgttc acgtgcagaa gtatatcagt gaagcgcgtc tgttattagc
     5881 attagcaatc ccggtgattc tcgcgcaaat cgcccaaact gcgatgggtt ttgtcgatac
     5941 cgtgatggcg ggcggctata gtgccaccga catggcggcg gtcgctatcg gtacttctat
     6001 ctggcttccg gcgatcctct ttggtcacgg actgctgctg gcattaacgc cggttatcgc
     6061 gcaattaaat ggttccggtc gacgtgagcg cattgcgcat caggtgcgac agggcttctg
     6121 gctggcgggt tttgtttccg ttctcattat gctggtgctg tggaatgcag gttacattat
     6181 ccgctccatg gaaaacatcg atccggctct ggcggacaaa gccgtgggtt atctgcgtgc
     6241 gttgctgtgg ggcgcgccgg gctatctttt cttccaggtt gcccgtaacc aatgtgaagg
     6301 tctggcaaaa accaagccgg gtatggtaat gggctttatc ggcctgctgg tgaacattcc
     6361 ggtgaactat atctttattt atggtcattt cggtatgcct gagctcggtg gcgttggttg
     6421 tggcgtggct actgcggcgg tgtattgggt catgttcctt gccatggttt cttacattaa
     6481 acgcgcccgc tcgatgcgcg atattcgtaa cgaaaaaggc accgcgaaac ccgatcctgc
     6541 ggttatgaaa cgactgattc aactcggttt gccgattgcg ctggcactat tctttgaagt
     6601 gacactgttt gccgtcgtgg ctctgttagt gtctccgctc ggtattgttg atgtcgcagg
     6661 acaccagatt gccctgaact ttagttcact aatgttcgtg cttccaatgt cgctggcggc
     6721 agcggtaact atccgcgtag gttatcgtct gggtcagggc tcaacgctgg atgcgcaaac
     6781 cgctgcgcgg accgggctta tggtgggtgt ctgtatggca accctgacgg ccatattcac
     6841 ggtttcactg cgggagcaaa tcgccctgtt gtacaacgac aatcccgagg ttgtaacgct
     6901 ggcggcgcat ttgatgttgc tggcggcggt atatcagatt tctgactcaa tccaggtgat
     6961 tggcagtggg attttgcgtg gttataaaga tacgcgttcc attttctata tcacctttac
     7021 ggcttactgg gtgctgggct tgccaagcgg ctatattctg gcactgaccg atctggtcgt
     7081 tgaacctatg gggccagcag gcttctggat aggctttatt attggcctga cgtcggcagc
     7141 cattatgatg atgctgcgta tgcggtacct gcaacgtctg ccgtcagcca tcattctgca
     7201 acgagcatcc cgctaataaa gacaaggcgc aaccttcacg gggtgcgcct gtgtttttac
     7261 gcaggctgga gcgttgcgcc aatcccgtct tcgtctggct gtagtttcag tgcgttacac
     7321 agagtttgcc gactggcatc tgttatctga acgcgtatat ccccgttaac ttcccctttc
     7381 acattgatga agtcaggtga aatttctttc gcactggttt gcaggcaaat cgctccctct
     7441 ccgcgcaggc gtttcacttt cagctgcaca tgcgttgccg caccagtgat cagccacagt
     7501 tttccgccat tcagattgat gttaccaata ctgaaagtga cactgtcacc ttttaccgta
     7561 ctgccgtcga gaatcaatac accgccctgc tgtacagtaa gcgttgattg ttgcagatct
     7621 gcgccatagc caataatcat tgaagcgccg tccagtaatg caacactccc ggtcagttca
     7681 accggtgcgc gatcttcctc tacgacaaaa ttttcgtccg cgctggtggg tgacaacagg
     7741 atcactgttc caccttgtat taccgcattt tccagcgtac cggcatgcac ttcagcccga
     7801 ccacccacat taacggacag gttttgcgtc ttaccggaac tgtagagctg aagaccatcc
     7861 gtccccagac gatagatggc tccattctca acaatagtgt cagagtcttc accaccgaga
     7921 ttattcatgg tgccatcagc aagcaacgta tcattggctt tccccgccag cataaaaacg
     7981 ccgccctgat taatctcaca attgctggct tccccacctg gatacaccaa caattcacca
     8041 ccttcatcta ccacgaccgc tgaggttgtc ccattaacca acaggccgcc ctcttgatcg
     8101 agaatgattt tttccgcgcg atgtccttcc agtacacgca ggttaccgcc attttccagc
     8161 aacaaaccgc aggcataacc ctgatcaacg ctgaattcac cttcgggatg gcggccatta
     8221 accgtagcga gcgtagagag actaattgcg cctccctcgg actgaacaat atctgtggca
     8281 atggcaccgt cgctgaccga tagcgtgcca ttgttaccgt actgtgtgca tttggcgatc
     8341 ccaccggttg ccactttcaa ccagccagcc cccgtgacca cggtattttc ggctcttccg
     8401 ccagcttcaa caatcaactc gccttcagta agttgcgtcg cgccaatgac ctcgccggtc
     8461 ttaacaattt gcacattccc gtcgctcatc aagtttttcg catcagatcc catgatttat
     8521 tcctttgctg catctgtgtg cctttattgc tacctaagtg taaaggctac ggaggattta
     8581 tccacgacag atttgagatg gtggcaaaca actctgttta aactctgata cacgaattat
     8641 tgggttgtat cagatgtaaa tgcgatcctg aataaaaatc gcccttgcaa atcaacaaaa
     8701 tatagccaat ttgcttaaaa gctctgcaaa cggtcgaaat agcgcagaaa attacgtttt
     8761 gcctcttgcc accttcccac tctgccgcta atattcgtcc ccgttgtcac ctacaacgtt
     8821 gcgttcatag ctcagttggt tagagcacca ccttgacatg gtgggggtcg ttggttcgag
     8881 tccaattgaa cgcaccatcc tgcgtccgta gctcagttgg ttagagcacc accttgacat
     8941 ggtgggggtc ggtggttcga gtccactcgg acgcaccaga ttttgttaat ctggtcttct
     9001 cctttttccc tctgcttctt ctctgtatcc aatacgttaa aagatttaca ctgtcttcgt
     9061 atgcgttatc agaaggagaa tcgctatggc aactttgtta caacttcatt ttgcttttaa
     9121 tggcccattt ggcgacgcaa tggctgagca gcttaaacca cttgcagagt cgattaatca
     9181 ggaacctggt tttctgtgga aggtatggac agaaagtgaa aagaaccacg aagccggtgg
     9241 gatctacctt ttcactgatg aaaaaagcgc ccttgcctat ctggaaaaac acactgccca
     9301 actgaaaaaa ctcggcgttg aggaagttgt cgccaaagtt ttcgatgtca atgagccact
     9361 gagtcaaatc aatcaggcaa aactcgcctg acagaattta atcaagggcg gttagcgccc
     9421 ttttcatccc tgtctgaaat ttctcaaatt ctaaaaatct caaccaaact tatctgataa
     9481 cactaaattc gaaagaatgc gtacaggtaa gtaacaatga aaaaaattgc aattgtgggt
     9541 gccgggccta cggggatcta caccttattc tcgcttctac agcaacaaac tctactttct
     9601 atttctatct tcgagcaggc tgacgaggcc ggtgtcggga tgccatacag tgatgaggaa
     9661 aactcaaaaa tgatgctggc aaatattgcc agtattgaaa taccgccgat ttattgtacg
     9721 tatctcgaat ggctacaaaa gcaagaagcc agtcatctcc agcgttatgg cgttaaaaaa
     9781 gaaaccttgc acgatcgtca gtttttaccg cgaattctgc tgggcgaata tttccgcgat
     9841 caatttttac gattagtaga ccaggcacga aagcaaaaat ttgcagtggc tgtttatgaa
     9901 tcatgccagg ttaccgatct gcaaattaca aatgctggcg tcatgctcgc tacaaatcag
     9961 gatttaccca gcgagacgtt tgatttagcg gtgatcgcca cgggtcacgt ctggcctgat
    10021 gaagaagaag caacccgaac gtattttcca agcccgtggt caggcttgat ggaagcaaag
    10081 gtcgatgcgt gtaacgtggg tattatggga acatccttga gcggactgga tgcggcaatg
    10141 gcagtggcta ttcagcatgg ttcgttcatt gaagatgata aacaacacgt cgtttttcac
    10201 cgcgataacg caagtgaaaa gctaaatatt acgttaatgt cgcgcacggg tattttaccc
    10261 gaagccgatt tctattgccc tattccctac gagcccttac acatcgtcac tgatcaggca
    10321 ttaaatgctg agattcaaaa aggcgaatat ggccttttgg atcgggtatt tagattgata
    10381 gtagaggaaa tcaagtttgc tgatccagac tggagtcaac gcatagcctt agagagcctg
    10441 aatgtcgatt cctttgctca agcctggttt gccgagcgca aacaacgcga ccaatttgac
    10501 tgggcagaaa aaaatctcca ggaagtcgaa cgcaataaac gagaaaaaca tactgttccc
    10561 tggcgttatg tcattctgcg cctgcatgaa gccgtacagg aaattgttcc acatctgaat
    10621 gaacacgacc ataaacggtt cagtaaaggc cttgcccggg ttttcatcga taattatgcg
    10681 gcaatccctt cagagtctat tcgtcgccta cttgccttac gtgaagcggg aatcattcat
    10741 attctcgctc tcggtgaaga ctacaaaatg gaaattaacg agtcgcgcac cgtcctgaaa
    10801 acggaagaca acagctactc gtttgacgtt tttattgatg cccgcgggca gcgtccgctt
    10861 aaagtgaaag atattccttt ccctggacta cgcgaacaat tacagaaaac aggggatgaa
    10921 atccctgatg ttggtgaaga ttatacgtta cagcaacccg aagatattcg tgggcgcgta
    10981 gcgttcggcg cgttgccctg gttgatgcac gaccagcctt tcgttcaggg acttacggca
    11041 tgtgcagaaa ttggtgaggc gatggctcgg gcggtcgtaa agcctgcatc ccgtgctcgt
    11101 cggcgtcttt cgtttgatta aaggtaaagc tatcagcact caacaaactg atgctgttca
    11161 ttaagacgat agcggactcc ggctcgaata ttgttttcac cttcaatggc gacggcgaag
    11221 cgaactcttt cgccgtcatg atatgccagt gcggcgctgc cccctggccc gagaataatt
    11281 gagtcgacca cgcccgtgct cactaccgtt gaattttcac cgctggcaat gatgtgtaca
    11341 ttgtcgccgc tgttggcaat tctggcgttt gcgccaaagc tggcgatttg caccaggtca
    11401 ccattactgg cgacatgaca ccgtttaccc aatgtacaaa cgcgtactcg cattccggta
    11461 ttcgcaatcc gactgctatc tccagcgctg cttatccgcg aagaattgcc ggttacagca
    11521 acccgtgcgc gctcaccgga gctgccaatg tgactgttaa agccgacact tccaatccgc
    11581 acgctataac ccatactggc aatttgtgca gcatacccag cggttgcgat tctggcgtta
    11641 tcctcacaac agccgatgcg agcatgatcg tcgctgcggg caaactgact gcaaaatgct
    11701 ctctctccgg ggtctgtggc cagtttttgc atagaggaaa cttcctgatg cgcgaagttt
    11761 tcatccgcca gccatttttt ccacgcatac tcaaccagac tctccgccca gtccgtatat
    11821 ccttcctcaa tcagtgcatg atgaatatcg gcataactac cgccgcgtgg gaaatggcgc
    11881 aggaaccagc gatacatcac cgctccgata cgccattctc gcaggtctgc tcgtgtaatg
    11941 atcattcgtt agtgccggtg atagccatcg accatgcttt taaaggtttc gtgtggcgta
    12001 cgccccgtgg tgcaaagata aagatgaccg aagataaaaa agaggcttat aaatgccaga
    12061 gcaaaatgcg cctgcaataa ccagtatctt acgccaggaa acacatctcc cacggcttgc
    12121 ggatagagac acagcagccc cgtcaatagt aacaacggca gcaatccata catgacacca
    12181 acataggcaa cctgctgtaa gggattaaat ttagactggg ttgttgccgg gaaaggatgt
    12241 tcttccccct gcataatgcc aaacaaataa aatcgcgttt gttttgccgc ccgctccagc
    12301 caaccctgac gacgaatgcg atagtggtgg ccattacccc caacgccatt gatcagaaca
    12361 aagccgagcc agcatgccag taacaaaaat ccgcaaactt catgcaccgc aaccagactt
    12421 ttaaccgcag ttgcgcccac cagcgcaaag tgatttatca gcccacttgc cagcaacaat
    12481 acaaagagta acgcattcga ccagtgccat aatctgaccg ctttgctgta aagataaatc
    12541 ctctcaccat gatcggtggc tgatttcttc acgccacgag cacgaagcaa ggcgtgcagc
    12601 cccagcacca accacatccc aaccagcaat actccggcaa tgatcaacca caccggccaa
    12661 aattcgggag tgaactgtgg cacatagttc gctaactggc tctgaaactg ttcagcatgt
    12721 tgcgacgggt tcatacattt tcctttttaa tcaaatgttg accgaaccgg cgatacagat
    12781 gcggtttccc ggcaccaggt agctgatatt gatagtattt attatcctgc aaccacgcct
    12841 gaatttcagg gctatcttcg cgaccaaaga tcaatgcgtg ttccgggcaa gcgctgacgc
    12901 aaatgggcgg aaagcctttt gccagtcggg actcagcgca gaaatcacat ttatctgcca
    12961 ctttagtgac agggttaagg tagcgaacct ggtacggaca agcgccaatg caataactgc
    13021 agccgatgca ctgcgatttt tctacccgca cgatcccctg ctcatcacgc cacgacgccc
    13081 ctgtcgggca tacgtcgatg cacggagcat cttcgcagtg ctgacatgac tggcgaaaaa
    13141 agtgatactg cgtttcgttg tcgttatcgg tgactggaat gtgtgctatc gataaacggc
    13201 ttccctgagc gggggcgtgg ttagttttac ggcatgcgca ggcgcaaata ttacagccat
    13261 tgcaccgtga ctcgtcatga atcatggcat accgaacttc cttttcttgc ctcgtgttcg
    13321 ctaacagaga agacgcagaa ccagtaaaaa agattactgt tcccatcccc agaacaaatt
    13381 tgcgtcgagt gaaggacatc tgattatccc ctaatgcagt ggctgcaact gtccacccta
    13441 tccatgacat ctccctgaaa tttcaggggt tctgaaactg ctctacctcg aaccattcgc
    13501 ataagccata agccttgcca taggtcataa atcgctcgct aaacaagtac aacaccgccg
    13561 tcgatgactc tacctgccgt atatcggcat attcaggtgc aacatccatt gcggcgatcg
    13621 cggcttcaat ctgcgcttcc tggaagtaat atggcgcttg catcagcatg gcgactttgt
    13681 aaggacgagg atacgtctgg cactcaaaac gaacggcgtg tgcaatggcg cgacaaatgt
    13741 cctgctccac aacttgcaaa gacatcgccg cgtagttttc actcatggcc tgcgttgagt
    13801 aatagtaatc atcctgactt cccgtcatac aagcgatatc cgcgtagtct ccgttctgct
    13861 tgatgttttc cagcaaaact gacagttcct cttctgcaac tgaatagggt ggcgagagaa
    13921 acacggcgcg cgccaccagc tgcccagcca cagaatgctg gcgaatgaac tctgccagga
    13981 tttcagccgg ttctggttcg gttaccgtat caacggggtg acgcatcccc tgcaaccatt
    14041 gccgcttcgc ctcgtcaact tcgctgactt tcgctaacgg caattcatcc tgatggttca
    14101 tcttacccat tccttacgca ggcagtagat tgtgtgctgc cagatcggcg gcaatatctt
    14161 ccagccccag acgctgcaat gtttcgcggg tcggacaacc aagctgtggg tcccagccca
    14221 tttctttgta gaacatcgtg agcgaggcat gcatatcatc acgatccatt ttgtcagtac
    14281 cttcagtaaa gaccgggatc tgcggatcct tgtcgaatac ccaggaacag ataagatcgt
    14341 gttcattacg catatctttg gtttgcatca gtttcaccgt gtaggcacga tgcaatgtaa
    14401 agatacgctc tgcagctaaa tctaattttt cctgagtcat ctcttcgcca gtgatcgctt
    14461 tgaagaattt ggcttcaagc gccagatcgc cccggtaatt acggcttttc agcggcgaaa
    14521 cggtcattgg ccagacccag ttgcacaggg tgacggcgtt atgcaaacag acccgcaaca
    14581 gcgaccactt agcatattta atttttgcgt cattgattgg cgtgtagttt ttggtttcat
    14641 cgtaagcatc ttcagaacca aaaagttctt tcgccacttc acgttgcagt ttcaatggca
    14701 agccggaacc aataaagttg atatgggtat gcgtcatgca gtcacggttg aacatacagt
    14761 taacaatgga accgacctgc gctgacgctt cattggcatg gtgaaccgga tagccaaacg
    14821 gcgaccagag tttatttttc gcgtagcccc agtactcttc acccaaattc cagcattctg
    14881 cgatggcata tgaaccatca gccaggtgac tcagctcacc cacacgatgc gccagacggt
    14941 agtaaaaatc tttaatgaag ttaacgtctc ccgcttccag ttgatcccag cgaatttctg
    15001 catactcttc agcaggcaga acacgcttga acacaccttt gctgtaacaa taagtaaagt
    15061 cgcgatgcaa ctgcccgtag ttacaccata ggccgtagtc atcgaacaaa ttcagaccaa
    15121 ccaggttacc aatcacacga ccatcgtctt tatcttcaaa atctttcggc ccgttcggga
    15181 agatggtggt atggacaaag tttgctacac aagtgttacc acctgtgctg ggcacgccaa
    15241 actctttcac ccgaggaata ttcatttggg tcatacaacg gatcgggcaa gagtggcagc
    15301 cgctcatttt tactgtgtat ttctctgctg ccggacctaa gtcaaaaacg gatttatagg
    15361 tacgaaagcc gaccgtattc tgattgcctg gcggaatttc acccgtttca attggaccgc
    15421 cttcagccgc gccccaaaac agccctttac gtgctgtcca gcgtgacttg ggatctgaat
    15481 actctgccca cgattgcggc gtacttggca cgacatggtt gttattcgca ccgataagtt
    15541 cagtcatcat gtaatcattg agacgcttca tctcctgacg atcggcaatg ttgacccctt
    15601 tcgtcccttc aaccgcaatc gctttcaggt ttttcgaacc cattattgcg ccagttcccg
    15661 caccgccgct gtggttacgg ctattcaaca tgccagagag aggaacaagg ttttccccag
    15721 cctgaccaat agccgccaca caggtttccg gactggtcaa gcgacaaatt tcttccgtcg
    15781 ttgcgcgcgt cccttttccc cataagaaat ctgctttttc caggctaact ttgtcatctt
    15841 taatcttcag ccataccggt gatttcgcct tcccttcgat aataatgacg tcgtatccag
    15901 cgaatttcat ttgcgctgca aaaaagccac ccatatgggc atcgacgact aaatttcctt
    15961 tggtaaaagt agaaagtgag gtgatattta cgcgagaact acagggggca ccagatccag
    16021 ttaatgggcc ggtagcaaag actaatttat tcgcttcatc gaaaggtttc gtgcctggcg
    16081 gtacttcgtc atacataatt ttgtagccga agcccatgcc accgacaaaa cttttaaact
    16141 tactggaatc ttcgagggta atatttcctg tcgtgagatt gactcttaat atattacctg
    16201 tccaaccgtt agtcatgatt ttttcctttg caagattcac acagtaatat ctttccactc
    16261 gataattttt aacgcccctg ttgggcaggc gtttgcgcat tcaccgcata acacacattt
    16321 cgaggatttt ttactttcgg tatttacggt ggccatcatc cacgggcatg ccgtagtaca
    16381 ggcgctacag ccaatacaac gtttatgatc gacggtaata cagccttctt tctgctgcca
    16441 ggtaatcgca ccaatcgggc agacgttcat gcattgcggc tctttgcatt gacggcaggt
    16501 gtccgcggta tagttgagat cgccatacag gccgccgcca gagccaaccc cgttgtcgcc
    16561 aaagaaataa ttgcgatgga ttttgatacg ggagaagaat gtccctactg agccatcgtt
    16621 gaagttggta caagagattt cacagcggtg gcaacccgtg catcgcgctc gctgggtcac
    16681 cagcacccct ttgggtgtat ttatcaaccc tactgtgccg ctatcaatat cttcctgttt
    16741 gcaacccaaa agtgacagta acgcaggggc aattgttaga ccagcaaggc cttttcctga
    16801 tatacgcaag aattctaatc gcgttaagcc aatatctaat agtggacgat caaccgggtt
    16861 catttatttc gcatcctctt cgcaaacaca gtgctgacaa ttcagcagcc tgatattaag
    16921 cattcaacta atacgctgct gactctgtcc ccaacaccag atattttccc taacccgata
    16981 gtgtatgaga aaatatttaa cgccgataaa ctaatccttt atggttagga gaaa
//
LOCUS       DBFIFM010000095        17015 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.095, whole genome shotgun sequence.
ACCESSION   DBFIFM010000095 DBFIFM010000000
VERSION     DBFIFM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.095"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..1558
                     /locus_tag="N7C74_002658"
     CDS             <1..1558
                     /locus_tag="N7C74_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HGU2432499.1"
                     /translation="NALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLLVVYLMTTRV
                     FRRQFSEMTELINTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAEIERSNK
                     LLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKIVRKLC
                     QRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAI
                     CEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIARNIRHAMEI
                     GELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDIGFINELGYQII
                     KTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLCVEI
                     TETVIERINEHFYLNIEQLRKQGLRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGD
                     IFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSA
                     WDKSGKLVKE"
     gene            1566..2894
                     /gene="dgcI"
                     /locus_tag="N7C74_002659"
     CDS             1566..2894
                     /gene="dgcI"
                     /locus_tag="N7C74_002659"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGU2432500.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(2964..3293)
                     /locus_tag="N7C74_002660"
     CDS             complement(2964..3293)
                     /locus_tag="N7C74_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2432501.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(3283..3669)
                     /locus_tag="N7C74_002661"
     CDS             complement(3283..3669)
                     /locus_tag="N7C74_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU2432502.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSGLSSKGSKEIEDDELAAYKKMAN
                     AFLAFSNKQIEDLIETGFLIEVKNER"
     gene            complement(3895..5220)
                     /gene="rimO"
                     /locus_tag="N7C74_002662"
     CDS             complement(3895..5220)
                     /gene="rimO"
                     /locus_tag="N7C74_002662"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HGU2432503.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            5433..5816
                     /gene="bssR"
                     /locus_tag="N7C74_002663"
     CDS             5433..5816
                     /gene="bssR"
                     /locus_tag="N7C74_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HGU2432504.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            5927..7042
                     /gene="yliI"
                     /locus_tag="N7C74_002664"
     CDS             5927..7042
                     /gene="yliI"
                     /locus_tag="N7C74_002664"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HGU2432505.1"
                     /translation="MHRQFFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(7039..7665)
                     /gene="gstB"
                     /locus_tag="N7C74_002665"
     CDS             complement(7039..7665)
                     /gene="gstB"
                     /locus_tag="N7C74_002665"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HGU2432506.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            7911..9113
                     /gene="dacC"
                     /locus_tag="N7C74_002666"
     CDS             7911..9113
                     /gene="dacC"
                     /locus_tag="N7C74_002666"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HGU2432507.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(9160..9918)
                     /gene="deoR"
                     /locus_tag="N7C74_002667"
     CDS             complement(9160..9918)
                     /gene="deoR"
                     /locus_tag="N7C74_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HGU2432508.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(9976..10572)
                     /gene="ybjG"
                     /locus_tag="N7C74_002668"
     CDS             complement(9976..10572)
                     /gene="ybjG"
                     /locus_tag="N7C74_002668"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGU2432509.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLLVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            10857..12089
                     /gene="mdfA"
                     /locus_tag="N7C74_002669"
     CDS             10857..12089
                     /gene="mdfA"
                     /locus_tag="N7C74_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HGU2432510.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            complement(12130..12414)
                     /gene="ybjH"
                     /locus_tag="N7C74_002670"
     CDS             complement(12130..12414)
                     /gene="ybjH"
                     /locus_tag="N7C74_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HGU2432511.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYHPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(12500..13315)
                     /gene="ybjI"
                     /locus_tag="N7C74_002671"
     CDS             complement(12500..13315)
                     /gene="ybjI"
                     /locus_tag="N7C74_002671"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HGU2432512.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKMVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKTLHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(13315..14523)
                     /gene="ybjJ"
                     /locus_tag="N7C74_002672"
     CDS             complement(13315..14523)
                     /gene="ybjJ"
                     /locus_tag="N7C74_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2432513.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALVGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            14607..15143
                     /gene="rcdA"
                     /locus_tag="N7C74_002673"
     CDS             14607..15143
                     /gene="rcdA"
                     /locus_tag="N7C74_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HGU2432514.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(15318..17003)
                     /gene="ybjL"
                     /locus_tag="N7C74_002674"
     CDS             complement(15318..17003)
                     /gene="ybjL"
                     /locus_tag="N7C74_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HGU2432515.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
BASE COUNT         4388 a         4090 c         4231 g         4306 t
ORIGIN      
        1 taacgcatta ttcgaaatct atttttatta tccgggcggt aatttattga acgcatcaga
       61 taaacttttt tatctgccgt ttgcgttcat tattatcgta ttgctggtgg tttatttaat
      121 gaccactcgt gtgttccgtc ggcaattttc tgaaatgaca gagctgatta atacgctggc
      181 gtttttgcct gactcaacgg atcagatcga ggctctgaaa attcgcgaag gcgatgcgaa
      241 agagattatc agcatcaaaa attcgatcgc ggaaatgaaa gatgccgaaa ttgaacggtc
      301 aaataaattg ctctcactga tctcttacga tcaggaaagt ggttttatta aaaatatggc
      361 gattattgag tccaacaata atcagtatct ggctgtgggg atcatcaaac tgtgtggtct
      421 ggaagccgtg gaagctgtgt ttggtgttga tgaacgcaat aaaatcgtca ggaaattgtg
      481 tcagcgaatt gctgagaaat atgcacaatg ctgcgatatc gtgacattca atgccgatct
      541 ctatttactt ctgtgtcggg aaaatgtaca gacatttacc cgtaaaatag cgatggtaaa
      601 cgattttgac agcagctttg gctaccgcaa tctgcgcatc cataagtctg ccatttgtga
      661 acctttgcag ggggaaaacg cctggagtta cgcagaaaaa ctgaaactgg cgatttccag
      721 tatccgtgac catatgttct ccgagtttat tttctgtgat gacgcgaaac tcaacgaaat
      781 agaagagaat atctggattg cgcgtaatat tcgccatgca atggaaattg gcgaactatt
      841 cctcgtctat caaccgatcg ttgatattaa cacccgcgcc attctgggcg cggaggcgtt
      901 gtgccgttgg gtgtctgcgg agcgggggat catttcaccg ctgaagttca ttaccattgc
      961 tgaagatatc gggtttatca atgagctggg ttatcagatt attaaaacgg caatgggtga
     1021 attcagacat tttagtcagc gtgcatcgct gaaggatgat ttcttactgc atattaatgt
     1081 ttcgccctgg cagttaaacg aaccgcactt tcatgagcgt tttaccacca tcatgaaaga
     1141 aaatggcctg aaggcgaaca gcctctgtgt tgagatcact gaaaccgtga tcgagcgaat
     1201 taatgaacat ttttatctca atattgaaca actgcgtaaa caagggttac ggatatcgat
     1261 tgatgacttt ggcaccggtt tgtcaaacct gaaacgtttt tatgaaatta atccagacag
     1321 cataaaggtg gactcgcaat ttaccggcga tattttcggt actgcgggaa aaattgtgcg
     1381 cattattttc gacctggcac gctataaccg gatcccggtg attgcggaag gcgtagagag
     1441 cgaagacgtt gcgcgcgaat taatcaaatt aggatgtgtt caggctcagg ggtatctgta
     1501 tcagaaaccc atgccattct ccgcctggga taaaagtgga aaattagtaa aagagtagtt
     1561 tacgtatgtc cagaatcaat aagttcgtac ttacagtcag tctgctgatt tttatcatga
     1621 tttcagcagt tgcctgcggg atctacactc aaatggtaaa ggaacgggtg tatagcctga
     1681 aacagtccgt tattgatact gcttttgcgg tggcaaatat tgctgaatat cggcgtagcg
     1741 tggcaattga tcttatcaac acgctaaatc ccacggagga acagctgttg gttggcttac
     1801 gcacagctta cgccgactcg gtttcaccct cttatttgta cgatgtcggt ccttatctga
     1861 tttccagtga cgaatgtatt caggtaaagg agttcgagaa aaattattgt gcagatatta
     1921 tgcaggttgt gaagtatcga catgtcaaaa atacagggtt tatctctttt gacggtaaaa
     1981 ccttcgtcta ttacctctat ccggtaactc acaatcgtag tctgatattt ttgcttggtc
     2041 tggagcgttt ttctttactg tcaaaatcgc tggcgatgga cagcgagaac ctgatgttct
     2101 ctctgtttaa gaacggtaaa ccggtgaccg gtgatgaata taatgctaaa aacgccatct
     2161 tcaccgtttc ggaagcgatg gagcacttcg cctatttgcc gaccggattg tacgtatttg
     2221 cgtataaaaa agatgtttat ttgcgggtct gtacattgat tattttcttt gccgcattgg
     2281 tggcagtgat atcgggtgcc agttgcctct atctggtacg cagagtgatt aatcgtggta
     2341 ttgtggagaa agaagccatt attaataacc attttgaacg cgtactggat ggcgggcttt
     2401 tcttttcggc tgccgatgtc aaaaaactct acagtatgta taactcggcg ttcctggacg
     2461 acctgaccaa agcaatgggt agaaaatcct ttgacgaaga tttaaaagcg ctgccggaaa
     2521 aaggcggtta tttgtgcctg tttgacgtcg ataaattcaa aaatattaac gacaccttcg
     2581 gtcatttgct gggcgatgaa gtgttgatga aagtggtgaa aatccttaaa tcacagatcc
     2641 cggtagataa aggtaaagtc taccgcttcg gcggtgacga atttgcggtg atttatactg
     2701 gcggcacgct ggaagagtta ctatcgattc taaaagaaat cgttcatttc caggtgggaa
     2761 gcattaattt aagcaccagt atcggtgtag cgcattcaaa tgaatgtact accgtcgaac
     2821 gcttgaaaat gctggcggat gagcggctgt ataagagtaa gaaaaacggc agggctcaga
     2881 ttagctggca gtagaattag aatagtgtta gtacccctga atcaaggggt actaatgaat
     2941 gtggtaaatc atttgcaaac attttacttt gcaaaaactt ccggcccttt tattgccaat
     3001 gtattcaaaa tacgtagtgc cgggccgctc ggttttattt cattacgttc ccatttcgag
     3061 acactttctt ttgacattcc cattgtatgt gccagcacgg attgtgacat gccgtatcgg
     3121 gtgcggatgg ctttgatctc ttcacccgtc attgatttga ctttggctat acgggcgctg
     3181 acttcacggg ctttgaccat gacggcaatg gcatcaacct cactatcaga catcaggccc
     3241 agtgcattca gttcctttgc atcttccagc atcatttgta atttatcgct catttttcac
     3301 ctcaatcaag aagccagttt cgatcaaatc ctctatttgc ttattgctaa aggccagaaa
     3361 tgcatttgcc atttttttgt aggctgccag ttcatcatct tcgatttctt tgcttccttt
     3421 actgcttaga ccactcttgg accaaccatc ataaaaaaac acatgatttg ctgatttaaa
     3481 aaagataatt gttctggctc caccactctt tccttgttgg aggggtaacc gttttttgat
     3541 gacaccgcta cctaaatcag cctcccatat gccttgcatc acttcttcag cggcagccat
     3601 gagaacttta tcgtgaatac caagtttttt cgtgctttgg ctgaatcgcc ttgccttata
     3661 cacacccata catccatcct tgtggtaatg tttactatag taaccattac gcttatttca
     3721 agtaattgcg atcaaaactt taatttggtg tgaaaaagtg agagttgcct cgagtacgat
     3781 tcatttttct cgatttatgc gtcaggggat tttgtgcgta agggtaggac atcgggaagg
     3841 gtagtagagg cagatcgaag aacaatatcc ttgttagatc tgcctttaga ttaattaaac
     3901 ccggctaccc cacaaatcgt actcatcggc gtgctcgact ttcacacgca ggatatcacc
     3961 cggcttaacg ttggtttcac cattgagata aaccgcaccg tcgatttccg gtgcatctgc
     4021 catgctgcga ccaatcgcgc cttcttcgtc cacttcgtcg ataatcacca gaatttcacg
     4081 gcccactttc tcttgcaggc gctcggcgga aatctgctgc tgcaactgca tgaaacggtt
     4141 ccagcgttct tctttcactt cttccggaac ctggtcaggc agggcattgg cgtctgcacc
     4201 ttcaaccggg ctgtatttaa agcagccaac gcgatccaga cgcgcttctt tcaggaagtc
     4261 gagtagcatc tggaaatctt cttccgtctc gccagggaag ccgacaataa aggttgagcg
     4321 tagggtcagt tccgggcaga tttcgcgcca ctgtttgatg cgcgccagtt ggcgatctac
     4381 agaacccgga cgcttcatca gtttgagaat gcgcgggctg gcgtgctgca acggaatgtc
     4441 cagatacggc aggattttgc cttctgccat cagtgggatg acgtcgtcca catgcggata
     4501 agggtaaacg tagtgcagac gtgtccagat ccccagtttc gataactgtt cgcacaggct
     4561 gaccatgctg gtttttaccg gctcgccgtt gtggaagcca gtacgatgtt taacatcaac
     4621 gccataggcg gaagtatcct gcgagatcac cagaatctct ttaacgcccg catctaccag
     4681 acgtttcgct tcacttaata cttcgccaat cggacggctc accaggtcgc cgcgcataga
     4741 cggaataatg cagaaggtgc agcggtgatt acagccttca gaaattttca gataggcata
     4801 atgacgcggc gtcagtttga caccttgttc tggcaccagg ctcaggaatg ggttgtgttt
     4861 cggttttggc acgtagtgat gaacgtgctc cagaacctgc tcatagctat gaggcccggt
     4921 gatttccagc actttcgggt ggacttcgcg gatctgatct tcttttgccc ccagacaacc
     4981 ggtcacaatt accttgccgt tttcattcaa cgcttcacca atggcttcca gtgattcttg
     5041 taccgcgctg tcaataaagc cgcaggtgtt gacgatcacc atgtccgcat cgtcatagct
     5101 cggtaccacg tcataacctt cagtgcggag ttcggtgaga atacgctctg aatcgacaag
     5161 gtttttcgga cagccaaggg aaacaaagcc gattttcggc tggggagtta ctttgctcat
     5221 agcttaaaaa atattcagtt acaggaaagg tcagggcagg gattctacag agttctggat
     5281 aaaatttgta tcgcaatctc attcgctggc ggaggcgaag gaaatgtaaa ttttgttaat
     5341 tcggcgtgaa gaattgatcc tggacagcat tatgctcaaa aaatagccat actatttaat
     5401 tgcaacaagg ctggaaagag gaggatcgaa gtatgttcgt tgacagacag cgaatcgatc
     5461 tgctgaaccg gttgatcgac gcacgcgttg acctcgccgc atacgtgcaa ctgaggaagg
     5521 caaaaggata catgtccgtc agcgaaagca atcatctacg agataacttt tttaaactga
     5581 atcgcgaact gcacgataaa tcgctgcggt tgaatcttca tctggatcag gaagagtgga
     5641 gtgctcttca tcatgctgaa gaagcattag cgacagccgc agtatgtttg atgagtgggc
     5701 accatgattg cccgactgtt attaccgtca acgccgataa gcttgaaaat tgtctgatga
     5761 gcttaacgct gagtatccag agcctgcaga agcacgccat gcttgagaag gcctgaaaac
     5821 taagggggag aaagcgtctc ccccttcatg tttaagtttt tgtaaaaatg aatttgttat
     5881 ctcctccact gactacgctt taagccagag tcaatccgga ggcgttatgc atcgacaatt
     5941 ctttttcctt gtgcccctta tttgtctttc ttccgctctc tgggcggctc ctgcaacggt
     6001 aaatgtcgaa gtactgcaag acaaactcga ccatccctgg gcactggcct ttttacccga
     6061 taaccacggt atgttaatca ctctgcgcgg cggcgagttg cgtcactggc aagcaggaaa
     6121 aggattatct gcgccgcttt ccggagttcc ggacgtttgg gcgcacgggc agggcggcct
     6181 gctggacgtg gttttagcgc ctgattttgc tcagtctcgc cgcatctggt taagttattc
     6241 cgaagttggc gatgatggca aagccggaac tgctgtgggg tatggccgct taagtgatga
     6301 tctctcaaaa gtgaccgact ttcgcaccgt ctttcgccag atgccaaaac tgtctaccgg
     6361 caaccatttt ggcgggcggc tggtattcga cggtaaaggt tatcttttta ttgctctggg
     6421 cgaaaacaat cagcgcccga cggcgcagga tctggataaa ttacagggca aactggtgcg
     6481 tctgaccgac cagggcgaaa tcccggatga taatcctttt ataaaggaat ccggtgcgcg
     6541 cgccgagatc tggtcttatg gcattcgtaa tccgcaagga atggcgatga atccgtggag
     6601 taatgcactg tggctgaatg aacatggccc gcgcggtggt gatgaaatta atatcccgca
     6661 aaaaggcaaa aactacggct ggccgctggc aacctgggga atcaactatt caggctttaa
     6721 gataccggaa gcgaaagggg agatcgtcgc cgggaccgag caacctgttt tttactggaa
     6781 agattcgccc gccgtgagcg gcatggcctt ctataacagc gataaattcc cccagtggca
     6841 gcaaaaatta tttattggcg cgctgaaaga taaagatgtc attgtgatga gcgtcaacgg
     6901 cgacaaagta acagaagatg gccgtatttt aacggacaga gggcagcgaa ttcgtgatgt
     6961 tcgcactgga cccgacggtt atttatacgt tctcaccgac gagtccagtg gggaattact
     7021 taaagttagc ccacgcaatt agctaacggg aatcatcacc actttgcgca ctgcggggcg
     7081 ttcagtgagt tgctgatacc agcgttgcag atttggacgc ggtgtccagg tcaggccaac
     7141 attaaacaaa ttgtagatga acggtgcgat agcgatatcg cccacaccaa actcgtcgcc
     7201 agagaaccat tttacttttg ccagttccgc atcgagcagg gcaaacaggg cgtcgcactc
     7261 tttgcaactg gcatcaatgg cggcctgatc gcgctcttcc ggtggtgttc tgactaatcc
     7321 catcaggatc ccgcgatgag cattactgag tgtctggttt gcccagtcca tccatttttc
     7381 tgcttccgca cgacgtgccg gtgagtcgat ccacaggcgt ttttgcccgt actgtgccgc
     7441 cagatagcgg acaatggcgt ttgattccca aagaatgaga tcactttcgt cgtcacgcaa
     7501 caacggcacc agcccgttag ggttcatcgc cagaaaatca gcatcgtgat ttatcccaaa
     7561 ctcacggccc gcgagaattt gctcataagg tagttccagt tcttcgagcg tcagcaatac
     7621 tttttttacg ttggttgaat tattccgacc ccacagcgta atcatactca ccccttttcc
     7681 gacaggcagc cacaggctga acaggactcc gatggtgaag taaagttaac ttttaagcaa
     7741 cagctggcaa aaaaatgaca ccagaagcag cgaagcggca ggatattgca taaactttaa
     7801 aaactttacc aacttacggt ttctttaagt ttgtgtgtgc gttattaatc accgaactta
     7861 tcatacggcg atataacgta tttttttgaa tggatactcg ggtggcattt atgacgcaat
     7921 actcctctct ccttcgtggt cttgcagcgg gttctgcatt tttattcctt tttgccccaa
     7981 cggcattcgc ggccgaacaa accgttgaag cgccgagcgt ggatgcgcgt gcatggattt
     8041 taatggatta cgccagcggt aaagtgctgg cagaaggcaa tgcggatgag aaactggatc
     8101 ccgcgagcct gactaaaatc atgaccagct atgtggttgg gcaggcgctt aaggccgata
     8161 agattaaact caccgatatg gtgacggtcg gtaaagatgc ctgggcgacg ggaaatccgg
     8221 cactgcgtgg ttcatcggta atgttcctca aaccgggcga tcaggtttcg gtggcagact
     8281 tgaacaaagg tgtgattatc cagtccggta atgacgcctg tattgcgctg gccgattacg
     8341 ttgccgggag ccaggagtca tttattggtt tgatgaatgg ttatgccaaa aaactggggc
     8401 tgaccaacac taccttccag acggtgcacg gtctggatgc gccggggcag ttcagtaccg
     8461 cgcgcgatat ggcattgctg ggtaaagcat tgatccacga tgtgccggaa gagtacgcca
     8521 ttcataaaga gaaagagttc accttcaaca aaattcgtca gcctaaccgt aaccgtctgc
     8581 tgtggagcag caatctgaat gttgatggca tgaagacagg aaccacggca ggcgcgggat
     8641 ataatctggt tgcttcggct acccagggcg atatgcgttt aatctccgta gtgctggggg
     8701 cgaaaaccga ccgtatccgt tttaatgagt ctgagaaatt attgacctgg ggtttccgct
     8761 tctttgaaac cgtgacgcca attaaacctg atgccacctt tgtgactcag cgcgtctggt
     8821 ttggtgataa gagcgaagtg aatctcgggg caggcgaagc gggctcagtg accataccgc
     8881 gtgggcagct gaaaaacctg aaggcgagct acacgttaac ggaaccgcag cttaccgcac
     8941 cgctgaaaaa aggtcaggtt gtcgggacca ttgatttcca gcttaacggt aaatccattg
     9001 agcagcgtcc gctgatcgtg atggaaaatg tggaagaggg cggattcttt ggtcggatgt
     9061 gggatttcgt gatgatgaaa ttccatcagt ggttcggcag ctggttctct taatcttctg
     9121 ataaccggat ggcgcgaaac gtcatccggt tatacgtcat taatacatca acttaatacg
     9181 ctgcgtctgc gcgtacttca catactcatc ttccgggcaa caatcgctca ccacaatatc
     9241 aaagcgtttc aggtcaccca tgcgcgccgg acgcaccttg ccaaatttac tgtggtcaac
     9301 aaccagcaca tgcttttgcg ccatcgacat ggcccagtgt tttaccggca actcttcaag
     9361 attaaaacag gtagcgcctt tactgacatg cacgcccgcc gcagaataaa aagcgatatc
     9421 cgggcaaaaa ttattcagcg tttgctgaaa atcgatgggt ttgaaaatgg cgttgctggc
     9481 gtgaaattca ccaccgcaca gaaatgcgcg gcaatggggt ttttctttca gcgccagaaa
     9541 ggtatttagc gaataacaaa cggcggtaaa agggatttca ttatcaatcg cttcaataat
     9601 ccacggcgtg gtggtgccac agtcaaaaaa gagggtctga tcgggttcta ccagtgtcgc
     9661 agccagtttt gccgcccggc gtttttcttc caccaggcgg gatttttgat cgcttaacag
     9721 gtaatggctg gcgctgcgcg gttccagaac aatatagccg ccgagcaaaa cgacgggcgc
     9781 actgtggttg ttcagatcgc gacgaatcgt catctccgaa accccaagca gggcggcggc
     9841 gtctttaaga tgtaacttat cgctgcgttt taattcttgc agcagctgcc cgatacgctc
     9901 ttcgcgacgt gtttccataa tccctctgaa tagttattga agcgagccgc tcaatactac
     9961 actttttagc agagatcagt cacgcaccca gcctttgcgg atcggtaatg caaaacagaa
    10021 gcgataccac gattgcagac gttgatagag tttatgcccc atcgcttgcc agataatctg
    10081 ggcactaagg cagccaatca tacctgccag caatccaccg agcatatcca gcggccagtg
    10141 gacgccaaga taaacgcgcg accaggcaat gacgacggcc agcaccatta aaagtgagcc
    10201 ggaccacagg cgatgccagc ataaaaatgc cagtgcaaag gtgaaaatca ccgtaccgtg
    10261 atcgctcggg aatgagtcat ccgccgcatg atgcaggaag ttatagccga tattttcgac
    10321 aaacggtcgg tcgtgcggaa aaagatgtcc catcgtccag gacaccaaca ggctgaccgc
    10381 cagcgcgata gcgattttta tcaccagttg ccgttgtgct gtaagccccc acaaccaaag
    10441 taccgcggcc agcagcggca ccacggtaat caaatcttta gcaataaaaa tcgccaacga
    10501 gatcatccac ggagccgagt ctggcgtcgc gttaataaga gagaatagag agagattcaa
    10561 attttccagc ataacttccc gacgcaaagt gattaaaagg gagccaatac aggcaagtcg
    10621 ttgagaataa agtgcaggtt aaactgggta aagcggcatc gtcttatttc cctcaagcgg
    10681 cctgtttacg gtgggtgatt gtaacgggca taggttaaat aaaacttaaa gaaagcgtag
    10741 ctatactcgc aataatgtaa gaatgtgctt aaccgtggtt tcagctacaa aattcgcttt
    10801 ctcgttagct gcgcttttat taaactctgc gcgattatta ttggcgaaga aattgcatgc
    10861 aaaataaatt agcttccggt gccaggcttg gacgtcaggc gttacttttc cctctctgtc
    10921 tggtgcttta cgaattttca acctatatcg gcaacgatat gattcaaccc ggtatgttgg
    10981 ccgtggtgga acaatatcag gcgggcattg attgggttcc tacttcgatg accgcgtatc
    11041 tggcgggcgg gatgttttta caatggctcc tggggccgtt gtcggatcgt attggtcgcc
    11101 gtccggtgat gctggcgggt gtggtgtggt ttattgtcac ctgtctggca atattactgg
    11161 cgcaaaacat tgaacaattc accctgttgc gcttcttgca gggcataagc ctctgtttca
    11221 ttggcgctgt gggatacgcc gcaattcagg aatccttcga agaggcggtt tgtatcaaga
    11281 tcaccgcgct gatggcgaac gtggcgctga ttgctccgct acttggtcca ctggtgggcg
    11341 cggcgtggat ccatgtgctg ccctgggaag ggatgtttgt cttgtttgcc gcattggcag
    11401 cgatctcctt tttcggtctg caacgagcca tgcctgaaac cgccacgcgt ataggcgaga
    11461 aactgtcgct gaaagaactc ggtcgtgact ataagctggt gctgaagaac ggtcgctttg
    11521 tggcgggggc gctggcgctg ggattcgtta gcctgccgtt gctggcgtgg atcgctcagt
    11581 cgccgattat catcattacc ggcgagcagt tgagcagcta tgaatatggt ttgctgcaag
    11641 tgcctatttt cggggcgtta attgcgggta acttgctgtt agcgcgtctg acctcgcgcc
    11701 gcaccgtacg ttcgctgatt attatgggcg gctggccgat tatgattggt ctgttggtcg
    11761 ctgctgcggc aacggttatc tcatcgcacg cgtatttatg gatgaccgcc gggttaagta
    11821 tttatgcttt cggtattggt ctggcgaatg cgggactggt gcgattaacc ctgtttgcca
    11881 gcgatatgag taaaggtacg gtttctgcgg cgatgggaat gctgcaaatg ctgatcttta
    11941 ccgtcggtat tgaaatcagc aaacatgcct ggctgaacgg gggcaacgga ctgtttaatc
    12001 tcttcaacct tgtcaacgga attttgtggc tgtcgctgat ggttatcttt ttaaaagata
    12061 aacagatggg aaattctcac gaagggtaaa aaaatgcctg attgctttgt gcgatcaggc
    12121 attctcgaat taatggtgat ggtcgtcaat ctggtgttcg ataaccatcc cttcacctac
    12181 gctggcaaga tggcgaacat aaggatgcgg gtggtaagcc ggagctggcg caggagccac
    12241 atagacggtt tgcggcacag tcgcgacgct gaccgcttgt ggaacgctga ctgaatcagg
    12301 aaccacaacc actttgtatc cactcggcac atcgacggtg acactttgcg ccatcgccac
    12361 accagtaaag cccattaaaa gcgcgcccaa cagtagacaa tttttcataa tcatttcgcc
    12421 tgaaataatg cagcgggtta atttccgcga attatgcagc cttcattcag gtattgtgtt
    12481 gccgggcgtg ctgccgtttt tattggtcaa atggcgcttc gtgtttaaga actttatcga
    12541 tcacatccag tacgccttca cggttattgg agcctgcccg gtattttgcc gctgcgacga
    12601 ccgcgttgcc ggcattttcc attgcaaaac taaagcctgc ctgacgcagc atctcaatat
    12661 cgttaccgcc atcgccaaag accaccactt cgctgtcgtc tattccccat aatttctgca
    12721 gttggcgaag gccattggct ttatgtacgc cggggataat cagatcgatg ctgccgttgc
    12781 cggtgtggac cggcaccata atatcgccga tggcctcatg taatgttttt tgtacttgtg
    12841 gaatcagttc atcggaaaga ttcaggccaa acttaaagaa gatatcttct aagttgtcaa
    12901 agttatcgac gtattccaga cggtgataat acatttccgc caccattttc atggcatcgt
    12961 catatttttt gagcgtatag gcgctatttt ttccgcaggc aataatttcc acttccgggc
    13021 gcgtcagcaa atgttccacg acagtagtaa acgcatcctt cgacagctcg ccattaaaaa
    13081 catctttgcc ttcactcact acccagccgc cgttttcagc cacaaaggca atttcattag
    13141 caatttcagg gaagaaagag atcaactgat aatattgatt cccgctggcg accacaaagc
    13201 gaatgccttg tgctttcatt tgctgatact gagccataaa ccgctcacgg ttataggttt
    13261 tttgatcgct taagaaagta ccatccatgt ctaccgcaat taatttaatg ctcatcaact
    13321 attctccatc gccgtctgcg ttttggtatc gggtttggcg acggctttcg cgacaatagc
    13381 cgcgagaata accagcgcca gtacaaccag cattgcacta cgtaatccat aatgttcgcc
    13441 gagatagccc agcagcggcg gcccgacgag gaaagccaga taaccggtcg ttgctaccac
    13501 actgacgcgg gttggtgcat cggggccggt atcgctggcg gcagaaatgg tcagcgggaa
    13561 gcccagcgag gcacccagtc cccagagtac aacagacacc ccagcgaccc aggcgctatc
    13621 gacaaaaata atcagcccaa tacccaacgc ccccattagc gcactggccc gaaccacggc
    13681 aacgcgactg taacggtcga tgaaccaacc gccagtgaag cgtccaacgg tcatccccag
    13741 ggtaaaaccg gcataaatca gcgagccgga agtggggcta aaaccgtgac cgtcaaccat
    13801 taataagggt aaccagtcgt tggcagaacc ttcggcaaag gccatcgcca gcaccacaac
    13861 accaatcagc agcaactgga tatcgcgata aaaaggtacg cctttttcgc catgctgggt
    13921 gccatcggca gcatttttgc ccgtaccgtc agggattgcc tgaatggcga tatatatagg
    13981 cgcgatgcct accagcgccg ccaataaaat gtgcaccgtt gccggaacgc caaaggccgt
    14041 cagtgccatc ccgacaccag cgcctgccag cgtgcccagg ctataaaaac cgtgcatcat
    14101 cggcaaaacc gttttattca tttctcgctc aacggcggca ccttcaacgt ttatcgccac
    14161 ttccgcagaa ccaaaacttg ccccaaagac gccgagacca acggcaaaga gcaggggcga
    14221 tgtcagccag agtgccagac ttaatatcat catcccgacc aatgcgcagg acatggtgac
    14281 caggatgaca ttacgtgtcc caaagcgttt cactaaccac gccgagcaga gaataccgct
    14341 catcgaaccg atcgacagac caaagagaac accgcccatt tcagcgatcg agacagagag
    14401 aatatcgcgg atagcaggcg tacgggttgc ccaggacgcc attaacaggc ccggcaaaaa
    14461 gaagaacata aacagcgccc aggttcggcg tttcaatgca ttacgtgaag aatttacggt
    14521 catagatcac gtcaaaataa gaagagggaa gacaacatta gcaaggttgt gtacatttgt
    14581 acacaattgc agacagagga aatgacatgc gtcgcgctaa cgatccgcaa cggcgagaaa
    14641 aaattatcca ggccacgctg gaggcggtga aactttacgg aatacatgct gttacgcacc
    14701 gcaaaattgc tacccttgcc ggggtaccgt tgggatcgat gacctactat ttttcaggaa
    14761 ttgatgagtt gttgctggag gcgttcagca gttttactga gatcatgtcc cggcaatatc
    14821 aggcattttt tagcgatgtt agtgatgctc aaggcgcatg ccaggctatc accgatatga
    14881 tctacagctc acaggttgca acgccggata acatggagct gatgtaccag ctctacgcgc
    14941 tggctagccg aaaaccgcta ttaaaaacgg taatgcaaaa ctggatgcag cgcagtcagc
    15001 aaacgctcga acaatggttt gaacccggaa ccgcccgcgc gcttgatgcg tttattgagg
    15061 ggatgacgct gcattttgtc accgaccgta agccgctatc gcgcgaggag attttgagga
    15121 tggttgagag ggttgcaggg tagtagataa atttcaggca acaaaaaacc catcaacctt
    15181 gaaccgaaat ggcggggttg atgggctcca caaaatgggg acatcaaaga aaagcagtgg
    15241 cactaattaa gactgatgcc ctgcggaaaa gttctgcggt tgtgcaaaaa aatttcattt
    15301 tcagggcaac ttcagtttta tcctaatcct ggccatacca tgacgatgat tgtccctgcc
    15361 agcgtcagca ggacgttggc gattgcgtag gtgcccgcat agcccagcgc cgggatgtta
    15421 ctgcgagctg tatcactgat gatctccatt gccggcgcgc aggtgcgtgc gcccatcatt
    15481 gcgccgaaca acagtgcgcg gttcattcgc aatacataag caccgaacaa gaaacagata
    15541 accacgggca caagactgac aatcaatccg gcaatcaaca tctgaccgcc aatcgcgccc
    15601 agaccgttat taataccgct accggcgctc agaccaacgc ctgccataaa caccatcaag
    15661 ccgaactctt tcaccatgct taatgcccct tgcggaatgt aaccgaaggt cgggtggtta
    15721 gcacgcataa agcccagcat aattccggcg aataacaacc cggcagcgtt ccccatgccg
    15781 aaactgaatg tgctgaactg gaaggtgatc atcccgatca tcagcccaat aacaaagaag
    15841 gcgcagaatg ccagcaggtc agtgacctgg ctgtgaatcg agataaagcc gatgcgatcg
    15901 gcgatggttt ttacgcggcg ggcatcgccg ctgacttgta aaacgtcacc tttgttaagc
    15961 acgacgttgt catcaatcgg catctcaatc tggctacgaa tgacgcggtt aaggaagcaa
    16021 ccgtgatcgg tcaacttcag ttgtgcgaga cgtttaccta cagcgttatg gtttttaacg
    16081 accacttctt cagtgacgat acgcatgtcg agaaggtcac gatcgaaaac ttctttaccg
    16141 ttacggaagc tgggatcgag tcgggcatgg gcgtcgggat agcctaccaa cgctatttca
    16201 tcgcccattt gtagcacggc atcaccgtct ggatttgcca gaatcccgtt acgtcgaata
    16261 cgttcaatgt agcagccggt ttgtcgataa atacccagtt cacgcagatt tttgccgtcg
    16321 gtccaggcca ccagctccgg gccgacgcga taggcgcgga tcaccggtaa ataaacctta
    16381 cggttggcat cagtgtccag gccacgttcg cgggcgattt gctgggcgct ggtctgtaag
    16441 tcctgatgct gcaatttcgg caagtaacgc gcaccaacaa tcaaactcac cagaccgatt
    16501 aaataggtta aggcataccc gaggctcaga ttatccagtg ccagtgagag ctgcctgctt
    16561 tccataccgg aatgacgcag tgtatcgcca gcaccgacca gaaccggtgt cgacgtcata
    16621 gagcctgcta acataccggc cgtcaggcca atatcccagc caaacagctt acctaaccct
    16681 aaggcgatca ccagcgcact gccaaccatc accagtgcta acattaggta atttttccca
    16741 tcgcgaaaaa aaatggaaaa aaagttcggt ccggcttcga ccccgacgca gaaaataaac
    16801 agcataaagc caagattaag cgcatcggtg ttaatgctga aatgttgttg gcctaataac
    16861 agcgatacga ctaaaacgcc aatggaatta cccagttgga tcgaaccaag tcgtaacttt
    16921 ccgagacata gcccaagcgc gaggaccaca aataataaca gaatgtaatt cccatttaac
    16981 aattcggcga cgtttatatt cacggaggct aactt
//
LOCUS       DBFIFM010000096        16917 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.096, whole genome shotgun sequence.
ACCESSION   DBFIFM010000096 DBFIFM010000000
VERSION     DBFIFM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.096"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..674
                     /locus_tag="N7C74_002675"
     CDS             <1..674
                     /locus_tag="N7C74_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HGU2432516.1"
                     /translation="RDHNCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNS
                     FGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYG
                     TPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAY
                     SDLPHDLKIGLRSLSSSAAIYSGLGFERVPQERDISCARMYLTPANHPDLWTQENGEW
                     IYLRN"
     gene            complement(745..1194)
                     /gene="rplI"
                     /locus_tag="N7C74_002676"
     CDS             complement(745..1194)
                     /gene="rplI"
                     /locus_tag="N7C74_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HGU2432517.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1236..1463)
                     /gene="rpsR"
                     /locus_tag="N7C74_002677"
     CDS             complement(1236..1463)
                     /gene="rpsR"
                     /locus_tag="N7C74_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HGU2432518.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(1468..1782)
                     /gene="priB"
                     /locus_tag="N7C74_002678"
     CDS             complement(1468..1782)
                     /gene="priB"
                     /locus_tag="N7C74_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HGU2432519.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(1789..2184)
                     /gene="rpsF"
                     /locus_tag="N7C74_002679"
     CDS             complement(1789..2184)
                     /gene="rpsF"
                     /locus_tag="N7C74_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HGU2432520.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            2511..2786
                     /gene="yjfY"
                     /locus_tag="N7C74_002680"
     CDS             2511..2786
                     /gene="yjfY"
                     /locus_tag="N7C74_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HGU2432521.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(2915..3601)
                     /gene="ulaF"
                     /locus_tag="N7C74_002681"
     CDS             complement(2915..3601)
                     /gene="ulaF"
                     /locus_tag="N7C74_002681"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HGU2432522.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(3601..4455)
                     /gene="ulaE"
                     /locus_tag="N7C74_002682"
     CDS             complement(3601..4455)
                     /gene="ulaE"
                     /locus_tag="N7C74_002682"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HGU2432523.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAVEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(4465..5115)
                     /gene="ulaD"
                     /locus_tag="N7C74_002683"
     CDS             complement(4465..5115)
                     /gene="ulaD"
                     /locus_tag="N7C74_002683"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HGU2432524.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(5129..5593)
                     /gene="ulaC"
                     /locus_tag="N7C74_002684"
     CDS             complement(5129..5593)
                     /gene="ulaC"
                     /locus_tag="N7C74_002684"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HGU2432525.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQEAVKIGVDLLVAADVVEPRYY
                     QAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(5603..5908)
                     /gene="ulaB"
                     /locus_tag="N7C74_002685"
     CDS             complement(5603..5908)
                     /gene="ulaB"
                     /locus_tag="N7C74_002685"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HGU2432526.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(5924..7321)
                     /gene="ulaA"
                     /locus_tag="N7C74_002686"
     CDS             complement(5924..7321)
                     /gene="ulaA"
                     /locus_tag="N7C74_002686"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGU2432527.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIILGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            7676..8740
                     /gene="ulaG"
                     /locus_tag="N7C74_002687"
     CDS             7676..8740
                     /gene="ulaG"
                     /locus_tag="N7C74_002687"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HGU2432528.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            8848..9603
                     /gene="ulaR"
                     /locus_tag="N7C74_002688"
     CDS             8848..9603
                     /gene="ulaR"
                     /locus_tag="N7C74_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HGU2432529.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(9600..10349)
                     /gene="yjfP"
                     /locus_tag="N7C74_002689"
     CDS             complement(9600..10349)
                     /gene="yjfP"
                     /locus_tag="N7C74_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU2432530.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEK
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGSGYFTSLARSLFPPLIPETVA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWYGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            10531..10860
                     /gene="bsmA"
                     /locus_tag="N7C74_002690"
     CDS             10531..10860
                     /gene="bsmA"
                     /locus_tag="N7C74_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HGU2432531.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTLQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            11009..11284
                     /gene="yjfN"
                     /locus_tag="N7C74_002691"
     CDS             11009..11284
                     /gene="yjfN"
                     /locus_tag="N7C74_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HGU2432532.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(11401..13026)
                     /gene="aidB"
                     /locus_tag="N7C74_002692"
     CDS             complement(11401..13026)
                     /gene="aidB"
                     /locus_tag="N7C74_002692"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HGU2432533.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDCAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(13110..14273)
                     /gene="yjfC"
                     /locus_tag="N7C74_002693"
     CDS             complement(13110..14273)
                     /gene="yjfC"
                     /locus_tag="N7C74_002693"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HGU2432534.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(14276..14914)
                     /gene="yjfM"
                     /locus_tag="N7C74_002694"
     CDS             complement(14276..14914)
                     /gene="yjfM"
                     /locus_tag="N7C74_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HGU2432535.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(14924..15322)
                     /gene="yjfL"
                     /locus_tag="N7C74_002695"
     CDS             complement(14924..15322)
                     /gene="yjfL"
                     /locus_tag="N7C74_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HGU2432536.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(15340..15999)
                     /gene="yjfK"
                     /locus_tag="N7C74_002696"
     CDS             complement(15340..15999)
                     /gene="yjfK"
                     /locus_tag="N7C74_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HGU2432537.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVVAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKENHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(16050..16748)
                     /gene="yjfJ"
                     /locus_tag="N7C74_002697"
     CDS             complement(16050..16748)
                     /gene="yjfJ"
                     /locus_tag="N7C74_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HGU2432538.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARTLEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVAATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(16767..>16917)
                     /locus_tag="N7C74_002698"
     CDS             complement(16767..>16917)
                     /locus_tag="N7C74_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HGU2432539.1"
                     /translation="GISSVQQEEYYIVFGALSLKSSLEDILLEITSLVDNALDLAEIT
                     EEYSH"
BASE COUNT         4282 a         4525 c         4086 g         4024 t
ORIGIN      
        1 gaagagatca taattgcgat ggcagacgta ctgttgagat ccacaaacta gatctctcag
       61 actggcaagc attcaataag ttagcaacga agtgtaatgc ttacgatgga gtaacaatga
      121 atggtgataa ttcttttgga tggaaccatg aagctacatt agataatatt cacgcccaga
      181 aatacaataa agcttacgcg ggtgcgaggt taaccgcaga actgaaatat ttattgcaag
      241 atgtcgaatc cttcgaaccc aacagtaaat acacaataca cgaagtggtt ttgggacctg
      301 gttatggtac tccagactat accggccaaa cgataggata cgttgtaacc cttcctgccc
      361 aaatgcctaa ttgctggtca tctgaactac caacaatcga tctttatatc gaccaacttc
      421 gcactgttac aggcgtttct aatgccctcg gattcatcat tgctgcattg ctaaacgcgt
      481 attctgattt accccatgat ttgaaaattg gtctgcgttc cttatcaagt tcagccgcaa
      541 tctactcggg tttgggtttc gagcgagtgc cgcaggagcg agacatcagt tgcgctcgta
      601 tgtatctaac gccagcaaac catcctgact tatggacaca agagaatggt gaatggatct
      661 acttgcgaaa ctaatacctg acgaaaaata gaaagcaaaa cgccgaccag tggtcggcgt
      721 ttttacgtct cgttgaataa cgaattattc agctactacg tttacgatca ctttcgcgaa
      781 tacttcgctg tgaacctgga agctcacttc gtgttcgcca gtggtacgca gaacgccgtt
      841 cggcagacga acttcgctct tagccacttc aacgccagct gcagttacag cgtcagcgat
      901 gtcgcgagta ccgatggaac cgaacagttt accttcgtcg ccagctttag acgcgatggt
      961 aacagtttcc agtgcattga ttttctcagc gcgagcatta gcagctgcca gaacttcagc
     1021 cagtttagct tccagttcag cgcgacgtgc ttcgaagaat tcaatgtttt tcttggtagc
     1081 tggaacagct ttaccctgtg gtaccaggaa gttacgagca tagcccgctt taacgtttac
     1141 ctgatcaccc aggctaccca ggtttgctac tttatcaagc agaataactt gcattacctt
     1201 atcctctcaa agtcgtatta atggaccgtg accgattact gatggcgatc agtgtacggc
     1261 agcagggaca ggtagcgagc gcgtttgata gcgcgagcca gctgacgctg gtattttgca
     1321 cgggtaccgg tgatacggct tgggacaatc ttaccgcttt cggtgatgta gtttttcagc
     1381 gtagcgatat ctttatagtc gatctcttga acgccttccg cggtgaaacg gcagaacttg
     1441 cgacgacgga aataacgtgc catatggcta gtctccagaa tctatcaatt caatctgctc
     1501 ggcatgcaaa accattttgc tcagtccgtt ctttgccttg tggcatgaaa tgaacccctg
     1561 aacggttatg cgactgccga ccgttatact gtgagtaatg gcctggtttt cgtgtccgct
     1621 aacaataacg ggcatttgac accatgcctg ccggtgaaag ccggcttcct cctgcacaga
     1681 acgatgctca agcacgaact ggcagtgagg aattcctgat ggactgacct ttcgaagggg
     1741 agtcctgcac acggtgccgg acaacaccag acggttggtc atcagaaatt actcttcaga
     1801 atccccagct tcagcatcat cagcggtttc gtttgcgaaa tcatcgcgac gctcacggcg
     1861 ctcgtctttc gctttaacca tcggagatgc ttcggtaaca gcgtgcttgg tacgcataac
     1921 catgctgcgg ataacggcat cgttgaagcg gaaggtagtt tccagctcat cgatcacttc
     1981 ctgcggagct tcaacgttca tcagaacgta gtgagctttg tgcagtttgt tgatcgggta
     2041 agccagctga cggcggcccc agtcttccag acggtggatc ttgccttctg caccagtgat
     2101 ggcagcagtg tagcgctcga tcatgcccgg aacctgttcg ctctggtcag gatggaccat
     2161 aaaaacgatt tcgtaatgac gcatcgaatt gctccttacg gattattcag cctcctgtct
     2221 gggtcagccg aatcccgggg aggcaaggaa cgtgttaaag gtcggctgaa aaatgacgcg
     2281 ttatagtact gacatacccc gctaaactca aggccagccg acaaataaat ctcatctttc
     2341 accaaagtgg actaacacgc tgatttgaag ttcatcacac ttcatcatgg tgttttttcg
     2401 aacaactgca tcagaaatgg tcaccatcaa cttctttatc attgattaaa attaaatcag
     2461 aagatcatca cagaggagag cacacaaagt cagccgtcga ggaggtatct atgttcagtc
     2521 gagttttagc ccttctggct gtgcttttgc taagtgcaaa tacatgggca gccattgaaa
     2581 ttaataacca ccaggccaga aatatggacg atgtgcaaag cttaggcgtg atttatatca
     2641 atcataattt cgccactgaa agcgaagcac gtcaggcatt aaatgaagaa acagatgcgc
     2701 aaggcgcaac gtactaccac gtaattctga tgcgggaacc ggggagtaac ggcaatatgc
     2761 acgccagcgc ggatatttat cgctagcacc aggtataacc aacgaaacat tgccatagtt
     2821 tgctttgccc ccttcgcagg gggctttttt tgaaaaacgg ctgcggaatt agaccagtta
     2881 tctcccgagg aaggaaattt ccgcagcgcg tgttctactt ctgcccgtaa taagcgttgg
     2941 ggccgtgctt acgcatgaag tgtttattca tcaggaagct gtcgatgtga ttgagttgtg
     3001 gattaatact gcgcgcaatc cacgccattt tcgccacttc ttccatcact accgcgttat
     3061 gcaccgcgtc gtgggcatct ttcccccagg cgaacggtcc gtgctgatac accacaattc
     3121 ccggcgtatg cagcggctcg gcgttgccca gcgtttcgat aatcactttg ccagtgttca
     3181 gttcatactc accctgcacc tcttcttcgc ttaatccacg cgtacacggg atatcaccaa
     3241 agaagtagtc agcgtgcgtg gtgcctaacg ccgggatcgc cagccccgcc tgcgcccatg
     3301 cggtagcatg agtggagtgg gtatggacaa tgccgccaag cgacggataa cgacggtaga
     3361 gttcgagatg cgtcgcggtg tcggaagacg gacgatattc cccttccacc accttaccgc
     3421 tcatatcgac caccaccata tcgtctgctt tcatggtttc gtaggcgacg ccgctgggct
     3481 tgatcaccac cagcccgcgt tcgcggtcga tagcgctgac gttgccccag gtaaaggtca
     3541 ccagcccata gcgcggcaga tccatgttgg cttcaaacac ctgctgtttt agcttttgca
     3601 ttatgccgcc tccaccatgc ccgctttcgc catgcgcgct ttcacccaat cacgcgcttt
     3661 cgccacttct gccgccgggt cttccgccgt ttcgctccac atctcaatca ggtacggccc
     3721 gcaatagcca ctctgtttga gcgtttcgaa gcaacgttcg aaatccacca caccttcgcc
     3781 aaatggcacg tttttgaaga cgcccggttt ggtgtctttc acatgtaccg cgacgatatg
     3841 cccgattccg gcctgcaact ccatctgcac atcgttgtcc cacgccgaca ggttgccgat
     3901 atccgggtag agctggaacc acggattgtt gagatagtgc gcgtagccca gcgccttgct
     3961 gatggagttc atcaacggat aatccatgat ctccaccgcc agcgtcacct gcgcgcggct
     4021 cgccatctca acgctctctt tcaggccgtc acggaaacga cgacgcgttt cgttattggc
     4081 ttcctgatag taaacgtcat agcccgccag ttggatcacg cgaataccaa cgtcctgggc
     4141 gaactggata gctttacgca taatctccag cccctgcgcc cgcaccgcgt catcttcact
     4201 gcccagcggg aaacgacgat gagcagaaag gcacatggac ggcacgcgca cgccggtttc
     4261 aacaatcgca ttcaccagcg ccagacgctg ctcgcggctc cagtcgaggc gcgacaggcg
     4321 ctcgtccgtt tcatctaccg acatctcgac aaaatcgaag cccaacgttt ttgccagttg
     4381 caggcgttcc agccagcact ccccggcggg gagcgctttt tcatagatgc caagcgggat
     4441 ttgtttggac aacatatccg ctccttagcc ccacagttcg gcgatggaac gtttgaactg
     4501 acgtgcggct tccaccggag aagcggcatc acggatacta cggcccgcga taaagacgtg
     4561 aatcggaatg cctttgaaca gcggcagatc ttccagcgcc aggccaccag tgacggtgac
     4621 tttgaagccc atatcggaaa gacgtttgat cgcggtgatg tccgcttcgc cccacgccac
     4681 gcctgcggcc tgcgcgtcac ggctgcggtg ataaaccacc tgctgaatac ctgcgtcgcg
     4741 ccactgttgc gcctgttccc aggtccagta accggtcagt tcgatctgca cgtcgccgtt
     4801 aaactctttt gccacgtcca gcgcgccttt ggcggtgttg atatccgcac agcaaattac
     4861 cgtcacccag tcagcgttgg cttcgaagca catacgcgaa aggattttgc ctgcatcggc
     4921 aattttggcg tctgccagta cgattttgtg cgggtagagc gctttcaggt cacgaaccgc
     4981 acgcacgcct tcgcccacgc acagaatggt gcccacttcg ataatgtcga cttcttcggc
     5041 aatcaggcga gtggtttcgt aggcgctatc catagtctgg ttgtccagcg cgacttgcaa
     5101 catcggtaat gacatgttca attccttctt aagctgccgc gttggtgcgg tcaatgagat
     5161 ccagtacttc ctgctcggta cggcaggcgc gtaaacggtc aaaattctct tcatcttcaa
     5221 acaggttgac gatctgcatg atgcccactt cctggtgagt attggcatcg accgccgcca
     5281 tggtgatgag gatatccacc ggatcgttgt catcgtggtt gaactccagc ggctttttca
     5341 gcgttaccag agagaaaccg gttttcttaa cgccctcttc cggacgcccg tgcggcattg
     5401 ccaggcccgg agcgataacg aaataaggac cgaactgttc aacgccatcc agaatcgcct
     5461 ggtagtaacg cggctctacc acatctgccg ccaccagcag gtcaacgccg attttcaccg
     5521 cttcctgcca tgtctctgct tcagcctgca gacggatgga tttattttca gccagcgaat
     5581 cacgtaattt catagcgcgt ccttacttca cgtcctgcgg gaaatgctct ttgatcactt
     5641 ccagcagttt cgggccaaag tcggcaggag agagcatgtt gcgcacgcca accacgtatt
     5701 tgttgccggt cacggtgatt tcgcccgcaa tgtgcgtaga agcgatgatg atatccgcgc
     5761 cactcaactc gcttttgtac tcgccaaccg cgcagctgtt taccgtatgg tcaatgtttg
     5821 attgggttaa aaactggtcc actttcatct tcatgatcat ggaactgcct tgtccgttgc
     5881 cacacacagc cagaatacgt acggtcataa tcaaaactcc ttattaagca gactgttctg
     5941 ccagttgttt ttctgcatct tcttctgcgc gcagcgcgcg gccagcgaag aacatataag
     6001 ccagtgcaat tacaatgatg acggccataa aggcgatacc gatggagaag aagccttgca
     6061 tcatcggcgg tgccagaatc gaccagtccg ccatgcccat ccaggcactc ataccggtga
     6121 gtttcaccgc ccagacgcaa ccaaagattt cgatcattcc catcaccaga caaatcttca
     6181 gcgctgcacg ccagccgccg aagtggttag cgaacacgcc gatggtggcg ttggagaaga
     6241 acatcgggat aaagcctaga ataatcagaa tggaagagcc gcaggcgacc aggatgccaa
     6301 ctgcaatcag ctgaccgatg gtgccccaca taaagcccca gaccacggcg ttcggcgcga
     6361 agctatagat agccgcacag tcaatcgcca gaaccgcacc tgggatcagg cgctgggaga
     6421 taccgttaaa tgcttcagag agttccgcca caaacatgcg cacgccctga gtgatgatga
     6481 agatcgccac cgcgaaggag aaaccggttt gcaggatgta gaccgtccag tgcactttgc
     6541 ccgccatcgc ctgcacggtg tcgataccga aggagagcag aatggcgcca aagaagatgg
     6601 tcatcacaat cgccgtggag acgatgttgt cgtggaaaat gttcagccag cccggcagtt
     6661 tgaggtcttc aacgctctcc tcttttttgc cgaggaacgg cgcgacttta taggcaatcc
     6721 atgatgcaaa ctgctgctgg tgaccgatgg agaaaccaca gccatccgtc acttcctgag
     6781 tcggcttgta catcatgttg gaagtgatgc cccagtagag cgaaaccaga atcgcggtac
     6841 agataatggt ggtccacatg gagtagccga agataaacag cgtaacggca atcaacccgg
     6901 cctgctggaa catgatgtgg ccggtcaaca tgattgtgcg aatgccggta atgcgacgca
     6961 acagcacgta acagatgttc agtgccagcg ctaacaatac ggcgtaaccc acccagctat
     7021 aggcatcgcc catgcggtcg atggttgcca tcattgaagc gtaggtatcg gaaattgcgc
     7081 cgttaatgcc gtagacttcg gacattttcg ccaccaccgg tttgaaggtg ctggtgagga
     7141 tgccggaccc tgcctgcaac aacatgaaac caattatggt tttaatcgtg cctttaataa
     7201 taacgctgac acttttgcgc agtaggatgt agcccagaca ggtcacaata cccagcaaca
     7261 acggggcatt ggtcatgacc tggttaaaaa atacggtaaa gatgttgtag aggatctcca
     7321 taacgatctc ctgaagaaga ggtaaccggg tattccacac acccggaatg ttgtgcactc
     7381 actctaattt tcaaaagtaa tcacaacaag attatttatg attaaatgtg acgcgacccg
     7441 caaataattc cacatggata gagcgggatt cacgcaacga aacaattatt catttaaatt
     7501 catttagtta attgaattat acattccatt aaatcttccc atactcaaaa tcaacaacaa
     7561 cttccctggc aaaaagtcaa aaaatggtca ttgccttcca gaaaaaatag tgtcaggatc
     7621 aatcaaaacc aatcacatat tgattcgaat tgaactatca tgaaggtaaa tggcgatgag
     7681 taaagtgaaa agtatcaccc gtgaatcctg gatcctgagc actttcccgg agtggggtag
     7741 ctggttgaat gaagaaattg aacaagaaca ggtcgctcct ggcacatttg cgatgtggtg
     7801 gcttggctgc accgggatct ggttgaaatc ggaaggtggc accaacgttt gcgttgattt
     7861 ctggtgcggc actggcaaac aaagtcacgg taacccgtta atgaaacagg gtcaccagat
     7921 gcagcgcatg gctggcgtga aaaaactgca gccaaacctg cgtaccaccc cgtttgttct
     7981 tgatccgttt gcgattcgcc agatcgacgc ggtactggcg actcacgatc acaacgatca
     8041 tatcgacgtt aacgtcgctg ctgccgtgat gcagaactgt gctgatgacg taccgtttat
     8101 cggaccgaaa acctgtgtgg atttgtggat tggctggggc gtaccgaaag agcgttgcat
     8161 cgtggtcaaa ccgggcgatg tagtaaaagt gaaagacatt gaaattcatg cgcttgatgc
     8221 tttcgaccgt actgcactga tcaccctgcc tgccgatcaa aaagcggctg gcgtactgcc
     8281 agatggcatg gacgatcgcg cggtgaacta cctgttcaaa acgcctggcg gctccctgta
     8341 tcacagcggc gactcccact actctaacta ttatgcgaag cacggtaacg aacatcagat
     8401 cgacgtggcg ttaggatcgt acggcgaaaa cccgcgcggt atcaccgaca agatgaccag
     8461 cgccgatatg ctgcgtatgg gtgaagcgct gaatgcgaaa gtggttatcc cgttccacca
     8521 cgatatctgg tcaaacttcc aggccgatcc gcaagagatc cgcgtgctgt gggagatgaa
     8581 aaaagatcgt ctgaagtatg gcttcaagcc gtttatctgg caggtgggcg gcaaatttac
     8641 ctggccgctg gataaagaca acttcgagta ccactatccg cgcggtttcg atgattgctt
     8701 cactattgaa ccggatctgc cgttcaagtc attcctgtaa tctgtcgttt ataccggata
     8761 gcgtggagaa gttcgcgcta tccggcgtag tatttcctgg ctatttcaaa tatcatctaa
     8821 aaaaatcaaa ttttatcgga atagctcatg actgaagcac aaagacatca aatcctcctg
     8881 gaaatgctcg cacaattggg ctttgtgacc gttgagaaag tcgttgagcg tctgggaatt
     8941 tcgcctgcca ctgcgcgacg cgatatcaat aaacttgacg aaagcggcaa actgaaaaaa
     9001 gtgcgcaatg gtgcagaagc tattacccaa cagcgcccgc gctggacgcc gatgaatctg
     9061 catcaggcgc agaatcacga tgaaaaagta cgtatcgcta aagcggcctc gcagctggtt
     9121 aatccgggcg aaagcgtagt catcaactgc ggctccaccg cgtttctgct tgggcgggaa
     9181 atgtgtggca agccagtgca aatcatcact aattatctac cgctggcaaa ttacctgatc
     9241 gatcaagaac atgacagcgt gatcattatg ggcggacagt acaacaaaag tcagtccatc
     9301 actttaagcc cgcagggcag cgaaaacagt ctctatgccg ggcactggat gtttaccagc
     9361 ggaaaagggc tgaccgcaga agggttgtat aaaaccgata tgctgacagc aatggcagag
     9421 cagaagatgc tgagcgtggt agggaaactg gtggtactgg ttgatagcag taagattggc
     9481 gaacgcgcgg gaatgctttt tagccgtgcc gatcaaatcg atatgcttat caccggcaaa
     9541 aatgctaacc cggaaatcct gcaacaactg gaagcgcaag gggtcagcat tctgcgtgtt
     9601 taaagatgct ggcggaaaaa tgtcacggca gcatctaacg cctcaggcgt aatgcggtga
     9661 cgcacgcctg gctgccatga acaggttagc agtttatccc gtcccgtctc gcttaaggcc
     9721 tgctgcaaac gtagtgattc gtcggcaggc acaacgtcat cgtcgaggcc ataccacagc
     9781 agtagaggtc tgtcactaag ttgttccagg tggtttgtcg cttcccactc tgccagtggc
     9841 gcgacaatgt tattgaattc attctgctgt gctaccgttt caggtatcag cggtggaaac
     9901 agtgaacggg cgagtgatgt aaaatagccc gatcccatca tgctggcggt acatctcacc
     9961 gtggggtggc gagcggtaat ccccagtgcc gtcatcgcgc ccatcgacgc accaccgact
    10021 gccagacggt catcaagcag ccacttttct tcggctattg ccgcacgtaa agtagtgaat
    10081 tcctgcatac tttgtagcaa gatttgccag aattgattta accgccgcgc tgcgtcacca
    10141 ctaaaacggc taccgtgatc gggcgcatcc ggcatgatca cccgcaaacc agcctgcgcc
    10201 agcgcaacgg caaaatagct atacaccaga ctggacgaag taaagccgtg ataaaaaatc
    10261 acgcacggta acggggtatc tttttgtccg acaggataag catgaagaac gggaatatct
    10321 gccagctcgc gtgattctat ttcaatcatc tgtctctcct ttttgtctgg tatgacatga
    10381 agaaatcctt agcacaagtt tctgctggcg gtattagttc ataatgttga gatgtgggtt
    10441 acgctttcgt cacgttttca ttcgaaaatc gcgtaccagg taacaattcg ggaacattcc
    10501 cccaaagtta aattaataag gcactacact atggttagca ggaaacgtaa tagcgttatt
    10561 taccggtttg ccagtttatt attggtgttg atgttaagtg cctgtagcgc actgcaaggt
    10621 acgctacagc cagcaccgcc agtaacggac catccgcaag agattcgccg cgaccagacg
    10681 caagggttac agcgaatagg tagcgtaagc acaatggttc ggggttctcc ggatgacgca
    10741 ttagcagaaa tcagagcaaa agctgtcgct gcaaaagctg attattacgt tgtcgtcatg
    10801 gtggacgaaa ccatcgtgac aggacagtgg tattcacaag ccattttgta ccgtaaataa
    10861 atcagacaac ttacatagtc agctttacat tgctttgcgt ccctatgcgt ttacctgtgc
    10921 acaataaatt accggctgga agcctgggcg tttattaccg cgatggaatg ccctgcaaca
    10981 tgtggggaaa cgaaaaatgg agctgacgat gaaacaatta cttgcctcac cctcgctgca
    11041 attagtgact tatcctgcga gcgccacggc acagtctgca gaattcgcca gtgctgactg
    11101 cgtgacgggc ctgaatgaaa taggtcagat ctcagttagc aatatttcag gggatccgca
    11161 ggacgtggaa cgtatcgtgg cgttaaaggc cgatgaacaa ggtgcgtcat ggtaccgcat
    11221 tattaccatg tacgaagacc agcagcctga taactggcgc gtacaggcga tactgtatgc
    11281 atgagtgtca tctacagcaa atgaacaatg ccggatgcgg cgcgagcgcc ttgtccggcc
    11341 tacaattttc acatatttca tttagttaat cgaaaccagc gtcgcatcag tcgtatacgc
    11401 ttacacacac actccccccg tcgcccgcag caataaatca ttctggatct gctctgacaa
    11461 ccgtacgccg ccgcgcgtat ctaacatcac ctgacaccac gcctgcgcca ttggcggaga
    11521 agcatatttc aacatttgcg caccgcagcc cagcaggaat agctgatgag taatctctcg
    11581 ccccagttct tcagctggct tacgcagctg ctgctgtaaa cgacgaaccg cgcaatcaaa
    11641 atagcgatcc tgccctttca cttctacaaa tgcttccgac aataagtcgt atacgcccgc
    11701 ttgcttattg agaacgcgca gcacatccag gcacataata ttgccggaac cttcccaaat
    11761 actgtttacc ggcatctccc ggtaaagccg cggcagctcg ctctcctcgc aataaccaat
    11821 gccgcccagc acctccatcg cttcggccac aaacggcata cctcgtttgc agatcacaaa
    11881 ttttgccgca ggcgtaaata aacgcgccca cagagcttct ttggcatcgg cgcgccggtc
    11941 ccacgctcgc gcaagacgaa acagcaacgc cgtttgccct tcaagctgaa gtgccatgcg
    12001 acttaagaca tgacgcataa ggggctgttg gatcaatgga ttaccaaaaa catggcgttg
    12061 atgcgcatga taaatcgcca gcgagaacgc acggcgcatc atggcatggc tacccagggc
    12121 gcaatcaaaa cgcgtcatcc cccccatttt caggatcaga cgaattcctt ccccttcctg
    12181 ccccaataac cagccaatgg catcctgaaa ctccacttca caactggcgt tagagcgatt
    12241 acccagctta tctttcagcc gctcgaggcg aatcgcgttg cgttgcccgt caggcaaaaa
    12301 gcgcggcaca aaaaagcagg acaaaccgcc cgcggtctgc gccagcacca gatgcgcatc
    12361 gctttgcgga actgagaaaa accatttatg ccccaccagc cgataagagc catcctccag
    12421 acgctctgcg cgggtggtgt tgctcatgac atcggaaccg ccctgctttt ccgtcattcc
    12481 catgccaatc aacaaaccgc gtttttgccc acctggcaat aagtgagaat cgtagcgatc
    12541 gctcaacagc ggtgtggtcc agtcctgaaa cggcgcgggt aacatctgta acaacaatgg
    12601 cgtggcggca aaggtcatgg ttatcggaca taacgacccc gcctcaacct gtgcgtgcaa
    12661 cataaaacgc gccgcgcgcg ccacaaatgc gccggagcga gcgtcttctt cccaggcaag
    12721 attgtgcacc cgattggtac atagtgcctg catcagcagg tgccaggcgg ggtgaaaacg
    12781 cacatcgtcc agacggcgtc cttgcgcatc gtagcgcaac agttcaggcg gattcacatt
    12841 cgccagccgc cccagttcaa gggattcagc cgttcctaac tgctgaccaa tactggctag
    12901 aaaatcgcta tcccagccag caccttcacg cgttaccgct tcgcaaagcg cgccatcaga
    12961 caggtataag ttgctgttat ttaatggtat aggttgatta aaaacggtgt gagtttgcca
    13021 gtgcactgtg tctccctcca tcaatggcag tcaccattag tatggtcact gccatggatt
    13081 catgacagaa agagattgct aaaacattct tatccagcaa tgtaatgcgg aacgaaacgt
    13141 gaggtgtctt tggtgatcag tgtgttatct tcacggatcc ccattccgca cgcttcatca
    13201 tcgacaatcc agctaccgat gagtgtgtag ctatcgccaa accgcggcag aggttgaaac
    13261 gcctggtaga tcatcggttc atcggcgtaa tcaccatcag cgtggtcaac gacgttattc
    13321 tggccgtcga aaatggtgac atttccgcct tcgcgcgagt agatcggttt acgcacatag
    13381 ctttcgccag cggcaatctg cggtttttca ccctcaaacc aggatgcaag aaggttagga
    13441 tgaccaggga agaagcgcca aagcagcggc attagccctt tattactcaa gatacttttc
    13501 cataacggct ccacccattg ctcgcgacgc ttgcgcagca gcggaccgtt atcgtcacgc
    13561 atcatccact ccagcggata cagcttaaat gcacgctgga tgacattatc atcaagatcg
    13621 gtcagtacgc cgccgacgcc caaaccgaga tcttcaatgt agataaaccg cgactcctgc
    13681 cctgcctgct gggcgcagtc ctgcaagtac agcacggtac tcctgtcttc atcggtgtcc
    13741 tgacagcagc aaaaataaaa cggttcccga ctgtaaagct cgctaaagcg tgaaatcagg
    13801 cgttcctgaa tagcattgta ctgatcggca tcacgcggaa taatgccgct gcgccgggca
    13861 tcctccagcc acagccactg gaaataagcc gactcgtaca atgaagttgg cgtatcggcg
    13921 ttgtactcca gcagcttcac cggcgcattg ccacaccagg caaaatccat ccgtccatac
    13981 agcgaaggat cgcgggcgcg ccagctttca gcgatcacat cccagtacaa cggcgggatc
    14041 gccagttgcg tcaggatctc ttcatctttt accgcgcgat ccaccacctc aaggcacatc
    14101 tgatgcagtt ccgcagtcgg tttttcgatc tgctcttcaa tctggcgcag agtaaaacgg
    14161 taagcccgac tctcatccca atagatttca ttgtcgatga tatgaaagtc gaaaccgttg
    14221 tcagcggcga tctggtccag atcccgtcgc acaggaacgt tgtgtctcag catgattagc
    14281 ctccccaatg cccacgggcg ctggaagaac gaccatagcc gccgcgagaa acggttgacg
    14341 cttttttggt ggtaaagccg cctgaagagt aagactcttt tttgccagaa ccggagcgcc
    14401 aggagtaatc accggaagtg ttgcgccaga cggggcggga agcaaatgag gatccgccgc
    14461 tgttatagac aaacggctca tcgcgatctt tacgaataac ccggcttaac aaaaatccag
    14521 aaacgaccgg gatccaactc tgttcaacat tgtcgtagta gcaattttcg tacttagact
    14581 gacagttctg ctgagtcata ttcttcggaa catcggcata aaatgccgct ttggcgttgt
    14641 tccagccacg cgcgcaaatg tcggcattat taccgtcatc aatacaatcc tgcactgtcg
    14701 aataaaacgt tccgtcgccg tcgttatcga catcgccact atcgccacaa cctttcaata
    14761 caaaaaaagc ggcaccgccc attagcgcca acgttaagta tttttgtgcg tagcgattgc
    14821 gacgcggttc aaaaggatta ttcggtctac caatgcgact gatcgctccg tgaccaattt
    14881 tactgttatt tcttgatttg cgttttctgg ccatgatcgt ccattaccat gtcatacaag
    14941 ctgcgttaaa aataccgcca gccagcgcgg cggttcccat aaacattcct gctgcggtat
    15001 tgtgattaat aattttttcg cttaatgcgg gcatataaag tctgacacca gcaaaaacga
    15061 gtaactgaat caccagtgca atcccgcccc aggcgaaata gtctggaata ctcaccgcat
    15121 tgattgccgc actggataag gggatcacgt aacccaacaa tgtaccgctg aacgccagtg
    15181 acgctgcggt attattgttt ttgattaatt gccattcatt gtgcggtgta attttggagt
    15241 agataaacag aaaaataatc accatagcca cgccaataaa aaaataggca ctaaaggcaa
    15301 gaagtgaatc cagtatatgc attgtaaacg tccttgtaat taaccaataa tatgaagtga
    15361 tgtcagcggg atatcgacac ccaacgcgcg cgaaaataac cactctggct cgccgagatc
    15421 gttaaaagat tcctcaccat ttagcagcaa atattcgtaa gtatcttcgg tcacttgccg
    15481 ctggtagccc atggtaaaat tatggacttc ccatttagca tgattttgat tttctacttt
    15541 ttccagcatg tagacgggtt cgatatttcc cggttcttcg ctgttaaaaa atcgctgcca
    15601 gcgtttttcc tgccagttta aggtcattgc ccccatcgtt ttggcgttga tcgcctcgcg
    15661 ccagtgattc tctttactga taccgtagct ctcttcatag acaaagagct tgatatcatc
    15721 aatgtcatca atatcttcgc cgccggtggt attgatttgc agaaactcat cgcctgaagt
    15781 gtagtagcgg aaaatctgac taccgccgcc cagatcgatg tggctgacag cgactaccgt
    15841 aaattcctca cccggcagcg caatcagcaa ttcatcttcc agcaaacgaa acgctaacgt
    15901 gtcgagcgta aagccactat tgagatgaag tcccagcgga ccgcgagcga tagcaggctt
    15961 attatccttg ccaaacagac gctggaaaaa accagacata ttttatcctt aaaatagttg
    16021 ccagccttgc agtgaggctg gcaaaaaaat tactcgccct gttggcgttg cagtcttgct
    16081 aatacatcct gggcgctact tttattgcta ccgccaattc cggcttccgc cagcttttcg
    16141 tcaaggtcgc gaccgtctgc gactttttct aactgtgcgg cagcatccag gcgagcctga
    16201 cgttcggcct gacgcgtttg caggcgtttt aaggattctg ctgctgtcga aacgctggaa
    16261 gatgcgccaa cggtagaagt cgttacagcc tgttgtgcac gctgcatggc ttcagtggct
    16321 ttaacgactt ccatttgctg ctcaaactga gcgatacgct gcgctgtcgc tgcaactgct
    16381 ttttccacgc catcacggga gacttccaga ttcgacaaca cttgctcttc agcggtaatg
    16441 agattctcaa ggcgcgcgat ttcttcagca acttcgttaa tcaacgacgg attaacgttc
    16501 ttgcttaacg cttccagcgt acgggcttcc agactggctt tgcgctcacg taaatctttc
    16561 agcttatcgt gacttaattt cacccgcgcc agcagatcaa cgcgagattt tccggcttta
    16621 tcgagttcag ctttagcgtc acgaatatgc tgttccagca tgcgcacacc ctgggtttct
    16681 tcaatggatt cttccgcctg ggagataaac gatttcccca gcgtaaataa actttttaaa
    16741 attcccataa atacccgtcc ttgtaattag tgtgaatatt cttctgtaat ttcagccaga
    16801 tccaatgcgt tatctaccag cgaggttatc tcaagcagga tatcttcaag agaagattta
    16861 agcgataacg ccccgaaaac aatgtaatac tcttcttgtt gtacgctgga gattcct
//
LOCUS       DBFIFM010000097        16847 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.097, whole genome shotgun sequence.
ACCESSION   DBFIFM010000097 DBFIFM010000000
VERSION     DBFIFM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.097"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            166..1509
                     /locus_tag="N7C74_002699"
     CDS             166..1509
                     /locus_tag="N7C74_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432540.1"
                     /translation="MRPISNLNTSAATYIPPQQLPSSCLETLTLLVQMNNYIKRDADY
                     STGMAVAPLVPEEVHLLAAAMTTELHRHQFQPVVSANDLQLPEPFTFDVAGFTITFTK
                     TQEHDNTGNLMKIAVSKSGVCTSTNITLELFHSIVTTLMSRSQYGTFDLWSVRPILTD
                     ESQNRVHEAARYSPAQQYGREETHFTNNGMREFGNMSPLAWRNDVELQQSCNTSNIPP
                     NAANDDINNTRQTIEDQPEADEPQQYVKLTVDDMRKWAAMDQQARNALQGVSGWCTRN
                     HFNIKNARNYLTDHGLNYAGQVKVNRPHEYAKFTLEHIRQWAALNKHVRKPVGYLEKW
                     CKERNLAPTTARNYLKNDGLTALGELKLTGPQKWVTFTFGDIVQWANMTQEERNSAGG
                     AEKWSKKRGFQWSTARSYLKSSGVTKQGARKLAWLKNSGNMSNPFYLAQPTSRRT"
     gene            complement(1658..3166)
                     /gene="putP"
                     /locus_tag="N7C74_002700"
     CDS             complement(1658..3166)
                     /gene="putP"
                     /locus_tag="N7C74_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HGU2432541.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPSVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            complement(3325..3534)
                     /locus_tag="N7C74_002701"
     CDS             complement(3325..3534)
                     /locus_tag="N7C74_002701"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432542.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            3589..7551
                     /gene="putA"
                     /locus_tag="N7C74_002702"
     CDS             3589..7551
                     /gene="putA"
                     /locus_tag="N7C74_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HGU2432543.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSTVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(7591..8229)
                     /gene="rutR"
                     /locus_tag="N7C74_002703"
     CDS             complement(7591..8229)
                     /gene="rutR"
                     /locus_tag="N7C74_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HGU2432544.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKSLIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            8517..9608
                     /gene="rutA"
                     /locus_tag="N7C74_002704"
     CDS             8517..9608
                     /gene="rutA"
                     /locus_tag="N7C74_002704"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HGU2432545.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPVLTQEVA"
     gene            9608..10300
                     /gene="rutB"
                     /locus_tag="N7C74_002705"
     CDS             9608..10300
                     /gene="rutB"
                     /locus_tag="N7C74_002705"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HGU2432546.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTS
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            10312..10651
                     /gene="rutC"
                     /locus_tag="N7C74_002706"
                     /pseudo
     CDS             10312..10651
                     /gene="rutC"
                     /locus_tag="N7C74_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
     gene            10659..11459
                     /gene="rutD"
                     /locus_tag="N7C74_002707"
     CDS             10659..11459
                     /gene="rutD"
                     /locus_tag="N7C74_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HGU2432547.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSITQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDHP
                     ASVTVLVCVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            11469..12059
                     /gene="rutE"
                     /locus_tag="N7C74_002708"
     CDS             11469..12059
                     /gene="rutE"
                     /locus_tag="N7C74_002708"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HGU2432548.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIIACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            12070..12564
                     /gene="rutF"
                     /locus_tag="N7C74_002709"
     CDS             12070..12564
                     /gene="rutF"
                     /locus_tag="N7C74_002709"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HGU2432549.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQN
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            12585..13913
                     /gene="rutG"
                     /locus_tag="N7C74_002710"
     CDS             12585..13913
                     /gene="rutG"
                     /locus_tag="N7C74_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HGU2432550.1"
                     /translation="MAMFGLPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASGFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGSSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(13996..14103)
                     /locus_tag="N7C74_002711"
     CDS             complement(13996..14103)
                     /locus_tag="N7C74_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HGU2432551.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            14475..15071
                     /gene="wrbA"
                     /locus_tag="N7C74_002712"
     CDS             14475..15071
                     /gene="wrbA"
                     /locus_tag="N7C74_002712"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HGU2432552.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMS
                     PQLFEKAGGKTQTAPVATPQELANYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            15092..15319
                     /gene="yccJ"
                     /locus_tag="N7C74_002713"
     CDS             15092..15319
                     /gene="yccJ"
                     /locus_tag="N7C74_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HGU2432553.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(15357..16598)
                     /gene="agp"
                     /locus_tag="N7C74_002714"
     CDS             complement(15357..16598)
                     /gene="agp"
                     /locus_tag="N7C74_002714"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HGU2432554.1"
                     /translation="MNKTLIAAAVAGVVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWRDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDADGFCLMDKFDSVLNEAVK"
BASE COUNT         3975 a         4506 c         4479 g         3887 t
ORIGIN      
        1 tttttacggg tgtttccatt aacaccagaa tgagcggatt gtttactaca tcaagaacgc
       61 atccgaagtg tacatcctgg tgcgccgact gaatcgcccg gagccatgcg gatggctacg
      121 acttaatacg aatcattaac cctgtaagga tacggagagc attacatgag accaatatcc
      181 aacctcaata cgagcgcagc aacctatatt ccaccgcaac aactcccgag tagttgtctg
      241 gaaactttga cgcttctggt gcagatgaat aattatatca aaagggatgc ggactacagc
      301 acgggcatgg ctgtagctcc actggttcct gaagaggttc acttgctggc tgctgcgatg
      361 acaaccgaac tgcatcgaca tcagttccaa ccggtggtaa gtgcaaatga tctgcaactt
      421 cctgaaccgt tcaccttcga tgttgccggt ttcaccatca ccttcacgaa gacgcaagag
      481 catgacaaca cgggcaatct catgaaaatt gcagtcagca agagcggtgt gtgcaccagt
      541 acaaatatta ctcttgagct gtttcactcc attgtcacaa ccctgatgtc acgcagtcaa
      601 tatggaacct ttgatctctg gtccgtacgg ccaatactca cggatgaaag tcaaaacagg
      661 gtgcatgaag cagcccgcta ctccccggcc cagcaatacg gaagagaaga aacacacttt
      721 accaacaatg ggatgcgaga atttggaaat atgtctccgc tggcgtggcg taacgatgtg
      781 gaattacagc aatcgtgtaa tacaagcaac atcccaccaa atgctgcaaa tgacgacata
      841 aacaacacca ggcagaccat cgaggaccag ccagaggcgg acgagccaca gcaatatgtt
      901 aaattgactg tagacgacat gcgaaaatgg gcggctatgg accagcaggc gcgaaacgct
      961 ctacagggag tttcaggatg gtgtacgcgc aatcatttta atatcaaaaa tgccagaaat
     1021 tacctgaccg atcacggcct gaactacgcg ggacaggtaa aggtaaacag gccgcatgaa
     1081 tatgccaaat tcacccttga acatatccgc cagtgggcag ccttaaacaa gcatgtgcga
     1141 aaaccggttg gttatttaga aaagtggtgt aaggaaagaa acctcgcgcc aactactgcc
     1201 aggaattacc tgaagaacga cgggcttacc gccctgggtg agttgaagct gacagggccg
     1261 cagaaatggg tcacgttcac cttcggagac atcgtacaat gggcgaatat gacccaggag
     1321 gagcgtaaca gcgcaggggg agccgaaaag tggagtaaaa aacgcggctt ccagtggtct
     1381 actgccagat cctacctgaa atcgagcgga gtgaccaagc aaggtgcgag aaaactggcg
     1441 tggttaaaaa acagcgggaa tatgtcgaat ccattctatc tcgcccaacc aaccagccgc
     1501 aggacataag ctgaaggtac cgatagcggc aattttgttc atctgtgccg ggtgggcaaa
     1561 tagcaattgc ccccgaacac tccctgccag gcaacttata ccgcatggca gggaacattc
     1621 gggttaaaaa gcaaaagata actaattact cttcagctta gccttcctgc aaccgtgacg
     1681 gcggagccga atgatagtgc gcatcggcct cggcaaagcg tttttgcatc gccgctgacg
     1741 gtgctttacc cagcaaacta aacactacaa tcccaatact gccaaagata aagcccggaa
     1801 taatttcgta cagtcccagc cagccgaact gtttccagac gatcaccgtc agcgcaccga
     1861 tgatcatccc cgccagcgca ccgttgcgcg tcatgcgtga ccacatcacc gaaaacagca
     1921 ccaccggacc aaacgccgcg ccaaagcctg cccacgcgta gctcactaag cccagcacgc
     1981 ggttttccgg gttcgctgcc aacgcaatcg ccaccagcgc caccaccagc accatcacac
     2041 gccctaccca caccagttct ttctggctgg catgtttacg cagaaacgct ttgtacaaat
     2101 cttcggtaat cgcactggaa cataccagca gctggcaact taacgttgac attaccgccg
     2161 ccagaatcgc cgacagcaga atcccggcaa tccacgggtt aaacagaatt tgcgccagtt
     2221 cgataaacac gcgctcggcg ttctggttta ccgcaccagc taccgacggg tgctcgttaa
     2281 agtaagcgat accaaagaag ccgacagcca ccgcccctgc caggcagagg atcatccagg
     2341 tcatactgat acgacgcgca tggacgatgc tgtggtgaga atccgccgcc ataaaacgcg
     2401 ccaggatgtg cggttgcccg aagtaaccca gcccccaccc catcagcgag ataatggcga
     2461 caaagttcag ccctttgagc atatccacgt tttcgatgct cttttgtttg atcacttcca
     2521 gcgagtcacc aaagccaccg acactgataa tgacgataac cggcgtcagg atcagggcaa
     2581 aaatcatcag gctggcctgt acagtgtcag tccagctcac cgcgaggaaa ccgccaataa
     2641 aggtgtaaag gatcgtcgcc gcagctcccg cccacagagc cgtttcgtag ctcatgccaa
     2701 aggtactttc aaacagacgc gcgcctgcca caatgcccga agcgcaataa atggtgaaga
     2761 acagcaaaat caccagcgcg gagataatgc gcaaaatgcg gcttttatct tcaaaacgcc
     2821 cggtgaaata atccggtaat gttaaggcgt tattgttgta ttcggtatgc acacgcaacc
     2881 gcccggccac cagcttccag ttaatccacg cgcctaatgt caggccaatg gcgatccaac
     2941 tttcggaaat cccggaaaga aaaaccgcgc ccggcaaccc cattaacagc cagccgctca
     3001 tatccgatgc acccgccgat aatgccgtca cgaatggccc aaggctacgt ccgcccagaa
     3061 tatagtcgtc aaagtttttc gttgatcgcc aggcgataaa cccaatcaat atcatgccaa
     3121 agatatagac acaaaatgtc accaacatcg gtgtgctaat agccatctaa agtctccaaa
     3181 aaattattat cggcaatgtc gaaacttgcc gttatatctg ccaccggaac ggggtaacag
     3241 agtttatgtt ttaccagggc gaccgtatcc tgccggaagc gctggttatt cacaatcgat
     3301 ttaacacacc atttacatta aattttagtg ctcagcgaca ctatttttca tcaggttgca
     3361 ctctctcaca ttttttgcgg ttgcaccttt caaaaatgtt aactgccgca gagaaaaagt
     3421 ctgagttatt tttttaatcc ctgtcatatc gatttctttt attaacattt cattcatttt
     3481 taagcttgct acgcaggtca catttaacat ggttgcacaa agttgcaaca tcatggatat
     3541 ttcacgataa cgttaagttg cacctttcag aacaacagga gtaatggcat gggaaccacc
     3601 accatggggg ttaagctgga cgacgcgacg cgtgagcgta ttaagtctgc cgcgacacgt
     3661 atcgatcgca cgccacactg gttaattaag caggcgattt tttcttatct cgaacaactg
     3721 gaaaacagcg atactctgcc agagctacct gcgctgcttt ctggcgctgc caatgagagc
     3781 gatgaagcac cgactccggc agaggaacca caccagccat tcctcgactt tgccgagcaa
     3841 attttgcccc agtcggtttc ccgcgctgcg atcaccgcgg cctatcgccg cccggaaacc
     3901 gaagcggttt ctatgctgct ggaacaagcc cgcctgccgc aaccagttgc tgaacaggca
     3961 cacaaactgg cgtatcaact ggccgataaa ctgcgtaatc aaaaaaatgc cagtggccgc
     4021 gcaggtatgg tccaggggtt attgcaggag ttttcgctgt catcgcagga aggcgtggcg
     4081 ctgatgtgtc tggcagaagc gttgttgcgt attcccgaca aagccacccg tgatgcgtta
     4141 attcgcgaca aaatcagcaa cggtaactgg cagtcacaca ttggtcgtag cccgtcactg
     4201 tttgttaatg ccgctacctg ggggctgttg tttactggca aactggtttc cacccataac
     4261 gaagccagcc tctcccgctc gctgaaccgc attatcggta agagcggtga accgctgatc
     4321 cgcaaaggtg tggatatggc aatgcgcctg atgggcgagc agttcgtcac gggcgaaacc
     4381 atcgcggaag cgttagccaa tgcccgcaag ctggaagaga aaggtttccg ttactcttac
     4441 gatatgctcg gcgaagctgc actgactgcc gcagacgcac aggcgtacat ggtttcctat
     4501 cagcaggcga ttcacgccat tggtaaagcg tctaacggtc gtggcatcta tgaaggtccg
     4561 ggcatttcaa tcaagctgtc ggcgctgcat ccgcgttaca gccgcgccca gtatgaccgg
     4621 gtaatggaag agctttaccc gcgtctgaaa tcactcaccc tgctggcgcg tcagtacgat
     4681 attggtatca acattgacgc cgaagaggcc gatcgtctgg agatctccct cgatctgctg
     4741 gaaaaactct gtttcgagcc agagctggca ggctggaacg gcatcggttt tgttattcag
     4801 gcttatcaaa aacgctgccc gctggtgatt gattacctga ttgatctcgc cacccgcagc
     4861 cgtcgccgtc ttatgattcg cctggtgaaa ggcgcgtact gggatagtga aatcaagcgc
     4921 gcgcagatgg acggccttga aggttatccg gtttataccc gcaaggtata taccgacgtt
     4981 tcttatctcg cctgtgcgaa aaagctgctg gcggtgccga atctaatcta cccgcagttc
     5041 gcaacgcaca acgcccacac gctggcggcg atttatcaac tggcaggtca gaactactat
     5101 ccgggtcagt acgagttcca gtgcctgcat ggtatgggcg agccgctgta tgagcaggtc
     5161 accgggaaag ttgccgacgg caaacttaac cgtccgtgtc gtatttatgc cccggtcggc
     5221 acacatgaaa cgctgctggc gtatctggtg cgccgcctgc tggaaaacgg tgctaacacc
     5281 tcgtttgtta accgtattgc cgatacctct ttgccactgg atgaactggt cgccgatccg
     5341 gtcactgctg tagaaaaact ggcacaacag gaagggcaaa ctggattacc gcatccgaaa
     5401 attcccctgc cgcgcgatct ttacggtcac gggcgcgaca actcggcagg gctggatctc
     5461 gctaacgaac accgtctggc atccctctct tctgccctgc tcaatagtgc actgcaaaaa
     5521 tggcaggcct tgccaatgct ggaacaaccg gtagcggcag gtgagatgtc gcccgttatt
     5581 aacccggcgg aaccgaaaga tattgtgggc tatgtacgtg aagccacgcc gcgtgaagta
     5641 gagcaggcgc tggaaagtgc ggttaataac gcgccaatct ggtttgccac tcctccggct
     5701 gaacgcgcgg cgattttgca tcgcgctgcc gtgctgatgg aaagccagat gcagcaactg
     5761 attggtattc tggtgcgtga agccggaaaa accttcagta acgccattgc cgaagtgcgt
     5821 gaagcggtcg attttctcca ctactacgcc ggacaggtgc gggatgattt cgctaacgaa
     5881 acccatcgtc cattagggcc tgtggtatgt atcagcccgt ggaacttccc gctggcgatt
     5941 ttcaccgggc agatcgctgc cgcactggcg gcaggtaaca gcgtactggc aaaaccggca
     6001 gaacaaacgc cactgattgc cgcgcaaggg atcgccattt tgctggaagc gggcgtaccg
     6061 ccaggcgtgg tgcaattgct gccaggtcgg ggtgaaaccg tgggcgcgca actgacgggt
     6121 gatgatcgcg tacgcggggt gatgtttacc ggttcaaccg aagtcgctac gttactgcag
     6181 cgcaatatcg ccagccgcct cgacgctcag ggtcgcccta ttccgctcat cgctgaaacc
     6241 ggcggcatga acgcgatgat tgtcgattct tcagcactga ccgaacaagt cgtaatagat
     6301 gtgctggcct cggcgttcga cagtgcgggt cagcgttgtt cggcgctgcg cgtgctgtgc
     6361 ctgcaagatg agattgccga ccacacgttg aaaatgctgc gcggcgcaat ggccgaatgc
     6421 cggatgggta atccgggtcg cctgaccacc gatatcggtc cggtgattga tagcgaagcg
     6481 aaagccaata ttgagcgcca tattcagacc atgcgtagca aaggccgtcc ggtgttccag
     6541 gcggtgcggg aaaacagcga agatgcccgt gaatggcaaa gcggcacctt tgtcgccccg
     6601 acgctgatcg aactggatga ctttgccgaa ttgcaaaaag aggtctttgg tccggtgctg
     6661 catgtggtgc gttataaccg taatcagctg ccggagctga tcgagcagat taacgcttcc
     6721 ggctatggtc tgacgcttgg cgttcatacg cgtattgatg aaaccatcgc ccaggtcact
     6781 ggctcagccc atgttggtaa cctgtatgtt aaccgtaata tggtgggcgc agtggttggg
     6841 gtgcagccat tcggcggcga agggttgtcc ggtaccgggc cgaaagcagg cggtccgctc
     6901 tatctctacc gtctgctggc gaatcgcccg gaaagtgcgc tggcggtgac actcgcgcgt
     6961 caggatgcag agtatccggt cgatgcgcag ttgaaagccg cattgactca gccgctaaat
     7021 gcactgcggg aatgggcagc aaatcgtcca gaattgcagg cgttatgtac gcaatatggc
     7081 gagctggcgc aggcaggaac acaacgattg ctgccagggc cgacgggtga gcgcaacacc
     7141 tggacgctgc tgccgcgtga gcgcgtattg tgtatcgcgg atgatgaaca ggatgcgctg
     7201 actcagctcg ccgccgtgct ggcggtgggc agccaggtgc tgtggccaga tgacgcgctg
     7261 catcgtcagt tagtgaaggc attgccatcg actgtcagcg agcgtattca actggcgaaa
     7321 gcggaaaata ttaccgctca accgtttgat gcggtgatct tccacggtga ttcggatcag
     7381 cttcgcgcat tgtgtgaagc agttgccgcg cgggatggcg caattgtttc ggtgcagggt
     7441 tttgcccgtg gcgaaagcaa tatcctgctg gaacggctgt atatcgagcg ttcgctgagt
     7501 gtgaataccg ctgccgctgg cggtaacgcc agcttaatga cgatcggtta aacaggtagc
     7561 cggaggatgt tacaccctcc tccggcatca ttagcgtggt cgaatcccct caataataat
     7621 ccgctgcacg ttttcaaccg tttgattgaa aaatacctca tcgcgcaacg ttgcgcctgt
     7681 caccgcctcc acctgagggg cgaaatcggc gtaatgttga gtggaagccc aaatcataaa
     7741 aatcaaatgc tgcggatcaa tcggcgcgag tttgccgctt ttgacccaac cggcaatcag
     7801 cgccgatttc tcatcaatta atgacttcaa atcgcccgtc agttcatcca ttaacagcgg
     7861 cgcgcctgcc agcatctcca tacagaacag gcgcgaagcc tgcggataat cgcgtgagac
     7921 ttccagcttc agacggatgt actctttgat cgccgccagc ggggcgaaat cttcacgaaa
     7981 cgcttttaac ggtgccagcc agatatcgag aatctgccgc agcacggcaa tatacagcgc
     8041 ctcttttgac ggaaagtaat acagcagatt ggtttttgaa acacccgcca actctgcaat
     8101 ttgctccagc cgtgtgccgt gaaaaccgaa ttgtgaaaaa gtgtccagtg ctgcgctaag
     8161 aatcgctttt ttcttcgcgc ttactgcgcg cgaacgttta cccgttgttt tcactgcgcc
     8221 ttgcgtcatg cgctctcccc tcttgtagat tggcaacagg atagcaaaac ccccgcccgg
     8281 cctcgacctt ctgcactctc atcgcgcaca gatgcatgtt ttatgtgcaa ctgttttgac
     8341 cgtttagtcc actttttacc agacatttta gataacgcga tgttaacaca ttaataacac
     8401 tcccttttta aaaactggca tccgctttgc aaacaagcca atacgcggct gaaaaggata
     8461 tgcagaatgc agcgccccgt ctgactttca ctttacgaga cgaagagagg ttgatgatga
     8521 aaattggcgt attcgtacct attggcaaca acggctggct catttcgacc cacgcgccgc
     8581 agtacatgcc gacctttgaa ctgaataaag ccatcgtgca aaaagcggag cactaccatt
     8641 tcgatttcgc cctgtcgatg atcaaactgc gtggctttgg cggcaaaact gagttctggg
     8701 atcacaacct tgagtcgttc accttgatgg cggggctggc ggccgtgacc tcgcgcattc
     8761 agatttacgc caccgccgcc accttaacgt tacctccggc aatcgtcgcc cgtatggccg
     8821 caaccatcga ctccatctct ggcgggcgtt ttggcgtcaa cctcgtgact ggctggcaaa
     8881 agcccgagta tgagcagatg ggtatctggc ctggcgatga ctatttctcc cgtcgttacg
     8941 actatctcac cgagtatgtt caggtgctgc gcgacctgtg gggcacggga aaaagcgatt
     9001 ttaaaggcga ttttttcacc atgaatgatt gtcgcgtcag tccgcaaccg agtgtcccca
     9061 tgaaagtgat ctgcgccggg caaagcgacg ctggcatggc gttctccgct cagtatgccg
     9121 atttcaactt ctgtttcggc aaaggcgtaa atacacccac ggctttcgcc ccgaccgctg
     9181 cgcggatgaa acaggccgca gagcaaaccg ggcgcgacgt tggctcttat gtattgttta
     9241 tggtgattgc cgatgaaacc gacgatgccg ctcgcgccaa atgggaacac tacaaagcgg
     9301 gcgcggatga agaggcgtta agctggctaa ccgaacaaag tcagaaagat acccgctccg
     9361 gtactgacac caacgtccgt cagatggccg atcccacttc ggcggtaaac atcaatatgg
     9421 ggacgttagt cggttcttac gccagtgtcg cgcgcatgtt agatgaagtc gcaagcgtgc
     9481 ctggtgccga aggcgtgctg ttaaccttcg acgattttct gtcgggaatc gaaaccttcg
     9541 gcgagcgcat tcaaccactg atgcagtgcc gcgcccatct ccctgtgctg actcaggagg
     9601 tggcatgatg acgacattaa ccgctcgacc ggaagccatt accttcgatc cgcagcaaag
     9661 tgcgctgatc gtggtggata tgcaaaacgc ctatgccacg ccaggcggct acttagatct
     9721 cgccgggttt gatgtctcaa ccactcgccc ggtcattgcc aacattcaaa ccgccgtgac
     9781 cgcagcgcga gcggcaggga tgttgatcat ctggtttcaa aatggctggg atgaacagta
     9841 tgtcgaggct ggcggccctg gctcaccgaa ttttcataaa tcgaacgccc tcaaaaccat
     9901 gcgtaaacag ccgcagctgc aggggaaatt gctggcgaaa ggctcctggg attatcaact
     9961 ggtggatgaa ctggtgccgc agcctggcga tattgtgctg ccgaagccgc gctacagcgg
    10021 tttcttcaat acgccgctgg acagcatttt gcgcagccgc ggaatacgcc atctggtttt
    10081 caccagcatc gctaccaacg tctgcgtcga atcgacgcta cgcgatggct tttttctgga
    10141 gtatttcggc gtggtgctgg aagacgcaac acaccaggcg gggccggaat ttgtacagaa
    10201 agccgcgttg ttcaatatcg aaaccttttt tggctgggtc agcgacgtcg aaacgttctg
    10261 cgacgcgctt tctcccacgt cctttgctcg tatcgcttaa ggagtttaac gatgccaaaa
    10321 tccgtaatta ttcccgctgg cagcagcgca ccgctggccc ccttcgttcc cggcacgctg
    10381 gctgatggcg tggtgtatgt ctccggtacg ctggcttttg atcaacacaa taacgtgctg
    10441 tttgccgatg acccaaaggc gcaaacccgc cacgttctgg aaactatccg caaggtaatc
    10501 gagacagcgg gtggcacgat ggcggatgtg accttcaaca gcatctttat taccgactgg
    10561 aaaaattacg gcgcgattct gcattcagtg cggactggta aaacctgacg cgctggtgga
    10621 aatcgccaca attgcgcata tcgccaagtg aggccgcgat gaaactttca ctctcacctc
    10681 ccccttatgc tgatgcgccc gtagtggtgt tgatttcggg tcttggcggt agcggcagtt
    10741 actggttacc gcaactggcg gtgctggagc aggagtatca ggtagtctgt tacgaccagc
    10801 gcggcaccgg caataatccc gacacgctgg cagaagatta cagtatcacc cagatggcag
    10861 cggaactgca tcaggcgctg gtagccgcag ggattgagca ttacgcagtg gtcggccatg
    10921 cgctcggtgc gctggtggga atgcagctgg cgctggatca tcccgcgtcg gtaactgtgc
    10981 tggtctgcgt taacggctgg ctacgaataa acgcccatac gcgccgctgt tttcaggttc
    11041 gcgaacgatt actgtatagc ggtggcgcgc aggcatgggt ggaagcgcag ccgttgttcc
    11101 tctatcccgc cgactggatg gcggcccgcg cacctcgcct tgaggcagaa gacgcgctgg
    11161 cactggcgca ttttcagggc aaaaataatt tactgcgtcg acttaacgcc ctcaaacgcg
    11221 ctgactttag tcaccatgcg gatcgcatcc gctgcccggt gcaaatcatc tgcgccagtg
    11281 atgatctgct ggtgccatca gcatgttcca gtgaacttca tgccgccctg cccgatagcc
    11341 agaaaatggt gatgcgctat ggcggacacg cctgcaacgt gaccgatccc gaaacgttta
    11401 atgctctgtt actcaacggg cttgccagcc tgttacatca ccgtgaagcc gccctgtaag
    11461 gaattgctat gaacgaagcc gttagcccag gtgcgcttag caccctgttc accgatgccc
    11521 gcactcacaa cggctggcgg gagacacccg tcagcgatga gacgttacgg gagctttatg
    11581 ccctgatgaa atgggggccg acatcagcta actgttctcc ggcacgtatc gtgtttatcc
    11641 gcactgcaga gggaaaagaa cgtctgcgcc cggcgctttc cagcggcaat ctgcaaaaaa
    11701 ccctgaccgc gcccgtcacc gctatcgtcg catgggacag tgaattttat gaacggttac
    11761 cgcaactgtt tcctcacggc gatgcccgca gttggtttac ctccagccca caacttgccg
    11821 aagaaacagc gtttcgcaac agttccatgc aggcggctta tctgatcatc gcctgccgtg
    11881 cgctggggct ggataccggc ccgatgtcgg gttttgatcg tcaacacgta gacgacgctt
    11941 tttttgcagg cagcacgctg aagagcaatc tgctgattaa tatcggctat ggcgatagca
    12001 gcaaactttt tgcgcgcctg ccacgtctgt cctttgaaga agcctgcggg ctgttgtaag
    12061 gagcaaacta tgaacattgt cgatcaacaa acttttcgcg atgcgatgtc ctgcatgggc
    12121 gcggcggtca atatcatcac cacggacggt ccagccgggc gcgccgggtt caccgccagc
    12181 gccgtctgca gcgtgaccga cacgcctccc acattactgg tgtgcctgaa tcgtggggcg
    12241 tccgtctggc cggtattcaa tgaaaatcga acgctgtgtg taaatacact tagcgccggg
    12301 caggagccgc tttcaaacct ttttggcggc aaaacgccca tggaacaccg ctttgccgcc
    12361 gcccgctggc agaatggtgt gaccggatgc ccgcaactgg aagaggcgct ggtttcgttt
    12421 gactgccgta tcagccaggt ggtgagcgtc ggcacccacg acattttgtt ttgcgccatc
    12481 gaagcgattc atcgtcacgc caccccctac gggctggtgt ggttcgatcg cagttatcac
    12541 gcgctgatgc gccctgcttg ttaacctcct taaggagaca gctgatggca atgttcggtc
    12601 ttcctcactg gcagttaaaa tcgacctcta cagagagcgg cgtggtcgcg ccggatgaac
    12661 gattgccgtt tgctcagacg gcagtaatgg gcgttcaaca cgcggtggcg atgtttggcg
    12721 cgacggtgtt aatgccgatt ctgatggggc tggatcccaa tctttccatt ttaatgtcag
    12781 ggatcggcac gttactgttt ttctttatta ccggcgggcg cgtgcccagt tatctcggct
    12841 ccagcgcggc ttttgtcggc gtggtgatcg ccgcaacggg ctttaacggt cagggcatta
    12901 acccgaatat cagcattgcg ctgggcggga ttatcgcctg tgggctggtt tataccgtca
    12961 ttggcctggt ggtgatgaaa atcggtacgc gctggattga acggctaatg ccgccggtgg
    13021 tgacgggtgc ggtggtgatg gcgattggcc tgaacttagc gcctatcgcg gtaaaaagtg
    13081 tatcggcttc aggctttgat agctggatgg cggtaatgac ggtgctgtgt attgggctgg
    13141 tggcggtgtt tacccgtggc atgatccagc ggttattgat tcttgtagga ttaattgtcg
    13201 cctgtctgct gtatggcgtg atgaccaacg ttttagggct gggaaaagcc gtcgatttta
    13261 ctctcgtcag ccatgctgcc tggttcggtc tgccacattt ttccactcct gcttttaatg
    13321 gtcaggcaat gatgctgatt gcgcccgtgg cggtgattct ggtggcggaa aacttaggtc
    13381 atctcaaggc ggtcgccggg atgaccggac gcaatatgga cccgtatatg gggcgagcat
    13441 tcgtaggcga tggtctggca acgatgcttt ctggttctgt cggcagtagc ggcgtcacca
    13501 cctatgctga aaatatcggt gtgatggccg tgacaaaagt ctactcgacg ctggtgtttg
    13561 ttgctgctgc cgtcatcgcc atgctgttgg gcttttcacc gaaatttggc gcgctgattc
    13621 ataccattcc ggccgcggtg attggtggtg catcaattgt tgtgttcggg ctgattgccg
    13681 tcgcaggcgc aaggatatgg gtacaaaacc gtgtcgattt aagccagaac ggtaatttga
    13741 ttatggtcgc agtaacctta gtgctgggcg cgggtgattt tgctctcacg ctgggcggtt
    13801 ttacgttggg aggaatcggt acagcaacct ttggcgcgat tttactcaat gcgttattaa
    13861 gcagaaggtt ggttgacgtt ccaccgccag aagttgttca tcaggagcca taaacagaaa
    13921 accgccgggg ttaccggcgg tgatggcttt ttgacgagct taagaaaaaa atgcattcgt
    13981 cattgattag cccggctagt tgtcgctttt gccgtgactg ctcttgccac cttttttacc
    14041 tgcttctgat gctctttcgc ggtcttctgc aaaattgccg gaaccgcctc gatggtttgc
    14101 cattactgac ctccgtctgg gtatagacat catattgcta gaattaacgc ggcgtatggc
    14161 ggctgccata ttttcgctac gtgggactaa gcttagtgaa ctgacgtgtt tctacccgcg
    14221 tttggtaata tcctgcaaca gcttcttagc gagaatagat atcatctcca ataattatcc
    14281 ataagccgct cttatgatta aaaaaacgcg atgagataaa aatgtgtcat tttgcgacaa
    14341 aattgcgtgc ttgtgaaagt tgttataaat caaataagtg gttgtgaaat ttgcactctg
    14401 aaaaggacgt cttatcttta aataagtggt agcgaatcgc tacggaatag agataacacg
    14461 aggagtggtt agaaatggct aaagttctgg tgctttatta ttccatgtac ggacatattg
    14521 aaacgatggc acgcgcagtc gctgagggtg caagcaaagt ggatggcgct gaagttgtcg
    14581 ttaagcgtgt accggaaacc atgtcgccgc aattatttga aaaagcaggc ggtaaaacgc
    14641 aaactgcacc ggttgcaacc ccgcaagaac tggccaatta cgacgccatt atttttggta
    14701 cacctacccg ctttggcaac atgtccggtc aaatgcgtac cttcctcgac cagacgggcg
    14761 gcctgtgggc ttccggcgca ctatacggaa aactggcgag cgtctttagt tccaccggta
    14821 ctggcggcgg tcaggaacaa accatcacat ccacctggac gacccttgcg catcacggca
    14881 tggtgattgt ccccattggc tacgcagcgc aggaattatt tgacgtttca caggttcgcg
    14941 gcggtacgcc gtacggcgca accaccatcg caggcggtga cggttcacgc cagcccagcc
    15001 aggaagaact gtctattgct cgttatcaag gggaatatgt cgcaggtctg gcagttaaac
    15061 ttaatggcta atcttcaaca ggaggatacg catgccaact caagaagcga aagctcatca
    15121 cgtcggtgaa tgggcatctc tgcgcaatac gtcgccggaa atagccgagg ccattttcga
    15181 agtcgccggg tacgacgaaa aaatggcgga aaagatttgg gaagaaggta gcgatgaagt
    15241 gttagtcaaa gcctttgcta agaccgacaa agattcgctt ttttggggcg aacagaccat
    15301 cgaacgtaaa aacgtttaac cagcgactcc cccgtagcga acgggggagt tttctgttat
    15361 ttcaccgctt cattcaacac gctatcaaac ttatccatca ggcagaaacc atcagcgtct
    15421 atcgggcaac cgcttaattc cagcgtcacg cgctgcgcag gtgcctgcag ggttaacgca
    15481 tcggcattac gtaactgttc cgcactctgg tacacatatt caattttcat caaatcgcga
    15541 ttggctttgc tgtcacgcca acgctggaaa acgattttgc cgccaatcgg cgtgcgttcg
    15601 ttctggtcat gcaactgata cggtttgaaa tccagcgccg ttaacagaga ggcaatgttg
    15661 gagtcatgcc caactaacac cgtgattttt ggagcactgg tgcgatcggt gaccagggct
    15721 ttgtcgatat aactgaccag cggtttcgca acattgcgcg ccacttccgg tgaggtaaac
    15781 aggctgtcct ggtaaccgtt tttcagcttc gacaacacct tccactgctg gtcagatttg
    15841 atttctcccc aggccacctg atccatcgga aaaccttcgt agtattgcaa agtaaacgca
    15901 tctaccagcg agttgccgac tttcagcggc ccggaaacac ctggttcttg ttgatacttg
    15961 gcgctaaagg tatttttgcc atccaccagc gaacactgtt gtttctcttt acaggcaggg
    16021 gaatctttat agttaacgat tttttccagt agctggtagc tgtcggtaag ctggagtttg
    16081 ctgagctctt tctccattgc cgccaccgcc tgttcactga atgcggcgga atcatcggtg
    16141 atcaccgggt taaaggttgg gtccatagtg cccatttttt cctggtgatg cacaggaata
    16201 tcacaccccg ggaatgcgcc ggtaataaag aactgtgcgg tcgcaacggt acgttgcaga
    16261 ctattggcat aggcataaac ggtgtccggc ggcgggcatt cccccgattt taccatcccc
    16321 tgctctgcca gccattcacg catgtaatgg cccatataca cttcgagtac gccacctttg
    16381 gtggtgagtt gcccaccggg gacgtcccat tcgggccatt tattcggcgt cgactgctcc
    16441 agcacactgc cattgttcgc cagcggcgca cgtaagttat ggcggctcat catgagcact
    16501 tgctgtagct gatagccttc cggtacggtt tgtgcctgag cgtttgaagc gagtaaaact
    16561 acccctgcca cagctgcggc gattagcgtt ttgttcattc ctggcacctc ttttgttatc
    16621 aatcactaaa gagtgtgaca gaaatatgac ttttttccgg gaacaggttc gcaaaatggc
    16681 ttgttgaacg gctgttttta tattcatctt taaaaacagg caaggtttga cgcaaactat
    16741 gcctgattag cccctgcctc gcgtacgccg ctgatgattg cacctgtggc taaacagaat
    16801 atcggcggcg ttaccaatac agaggcaata tttaccaata aaaaagt
//
LOCUS       DBFIFM010000098        16841 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.098, whole genome shotgun sequence.
ACCESSION   DBFIFM010000098 DBFIFM010000000
VERSION     DBFIFM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.098"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            38..412
                     /gene="yobA"
                     /locus_tag="N7C74_002715"
     CDS             38..412
                     /gene="yobA"
                     /locus_tag="N7C74_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HGU2432555.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKRLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            416..1288
                     /gene="copD"
                     /locus_tag="N7C74_002716"
     CDS             416..1288
                     /gene="copD"
                     /locus_tag="N7C74_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HGU2432556.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWKDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLLIISLLATLEPF"
     gene            1301..1642
                     /gene="yebY"
                     /locus_tag="N7C74_002717"
     CDS             1301..1642
                     /gene="yebY"
                     /locus_tag="N7C74_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HGU2432557.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDVAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            2038..2694
                     /gene="pphA"
                     /locus_tag="N7C74_002718"
     CDS             2038..2694
                     /gene="pphA"
                     /locus_tag="N7C74_002718"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HGU2432558.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLSERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTFVQLQ
                     "
     gene            complement(2695..2886)
                     /gene="yebW"
                     /locus_tag="N7C74_002719"
     CDS             complement(2695..2886)
                     /gene="yebW"
                     /locus_tag="N7C74_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HGU2432559.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(2991..3227)
                     /gene="yebV"
                     /locus_tag="N7C74_002720"
     CDS             complement(2991..3227)
                     /gene="yebV"
                     /locus_tag="N7C74_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HGU2432560.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(3345..4784)
                     /gene="rsmF"
                     /locus_tag="N7C74_002721"
     CDS             complement(3345..4784)
                     /gene="rsmF"
                     /locus_tag="N7C74_002721"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HGU2432561.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLRLTAPYGWTLTPIPWCEEGFWIERDDEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRSGGTLVYSTCTLNREENESVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAAQIRQAAASVGLNWDGNLRLWQRDKEVWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNENAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(4865..7498)
                     /gene="yebT"
                     /locus_tag="N7C74_002722"
     CDS             complement(4865..7498)
                     /gene="yebT"
                     /locus_tag="N7C74_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HGU2432562.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTNNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEACSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(7467..8750)
                     /gene="yebS"
                     /locus_tag="N7C74_002723"
     CDS             complement(7467..8750)
                     /gene="yebS"
                     /locus_tag="N7C74_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HGU2432563.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            8880..9377
                     /gene="msrC"
                     /locus_tag="N7C74_002724"
     CDS             8880..9377
                     /gene="msrC"
                     /locus_tag="N7C74_002724"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HGU2432564.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            9474..10172
                     /gene="proQ"
                     /locus_tag="N7C74_002725"
     CDS             9474..10172
                     /gene="proQ"
                     /locus_tag="N7C74_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HGU2432565.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTAKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            10192..12240
                     /gene="prc"
                     /locus_tag="N7C74_002726"
     CDS             10192..12240
                     /gene="prc"
                     /locus_tag="N7C74_002726"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HGU2432566.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            12432..13313
                     /gene="htpX"
                     /locus_tag="N7C74_002727"
     CDS             12432..13313
                     /gene="htpX"
                     /locus_tag="N7C74_002727"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HGU2432567.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(13359..14732)
                     /gene="yebQ"
                     /locus_tag="N7C74_002728"
     CDS             complement(13359..14732)
                     /gene="yebQ"
                     /locus_tag="N7C74_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2432568.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQILTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACISGLRI
                     TQPRSRA"
     gene            14909..15700
                     /gene="kdgR"
                     /locus_tag="N7C74_002729"
     CDS             14909..15700
                     /gene="kdgR"
                     /locus_tag="N7C74_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HGU2432569.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(15844..16083)
                     /gene="yobH"
                     /locus_tag="N7C74_002730"
     CDS             complement(15844..16083)
                     /gene="yobH"
                     /locus_tag="N7C74_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HGU2432570.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAELGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            16243..16386
                     /gene="mgrB"
                     /locus_tag="N7C74_002731"
     CDS             16243..16386
                     /gene="mgrB"
                     /locus_tag="N7C74_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HGU2432571.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            16461..16748
                     /gene="yebO"
                     /locus_tag="N7C74_002732"
     CDS             16461..16748
                     /gene="yebO"
                     /locus_tag="N7C74_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HGU2432572.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVEDEDIIRLVAER"
BASE COUNT         4383 a         4091 c         4313 g         4054 t
ORIGIN      
        1 ctctgtaaaa aagttattac aaaaaggaat agataatatg gcttcaactg cacgctccct
       61 tcgctacgcg ctggcaatcc tgaccacttc gttggtgaca ccttccgtct gggcacatgc
      121 gcatttaacg catcagtatc ctgcggcaaa cgcgcaagtg acagctgcac cgcaggcaat
      181 cactttaaac ttctcggaag gtgttgaaac aggattcagt ggtgcaaaaa tcacggggcc
      241 aaaaaacgaa aatattaaaa cattaccggc gaagcgaaat gagcaggacc aaaagcgact
      301 gattgttcca ctggccgatt cgctgaaacc cggaacctac accgttgact ggcatgttgt
      361 ttcggtggac gggcataaaa cgaaaggaca ctacaccttt agcgtgaaat aaatcatgct
      421 ggcgtttacc tggatcgcgc tgcgatttat ccatttcacc tcgctgatgc tggtctttgg
      481 cttcgcaatg tacggtgcct ggctggcacc cttaacgatt cgtcgcttgc tgacgaagcg
      541 ttttctgcgt ttacaacagc acgctgccgt ctggagtttg atcagtgcca ctgcaatgct
      601 cgccgttcag ggcgggttga tgggaacggg atggaaagat gtattttcgc caaacatttg
      661 gcaggctgtt ttgcaaacgc agtttggtgg cgtctggtta tggcaaattg ttctcgccct
      721 cgtaacgttg atcgtcgccc ttatgcaacc gcggaatatg ccacgtttgc tgtttatgct
      781 cactaccgcg caatttattc tgctggcggg agtggggcat gcgacgctga atgaaggggt
      841 aacggcgaaa atccatcaga ctaatcacgc gattcacctg atttgtgcgg cggcgtggtt
      901 tgggggatta ctgccggtgc tctggtgtat gcaactcatc aaaggccgtt ggcgacacca
      961 ggctattcag gcgctgatgc gtttttcctg gtgcgggcat tttgcggtga tcggcgtact
     1021 ggcaagcggc gtgcttaatg cattgttgat taccggattt ccccccacgc ttacgactta
     1081 ctggggccaa ctcttattgc tcaaagccat cctggtgatg attatggtgg tcattgcgct
     1141 ggctaatcgt tatgttcttg taccgcgcat gaggcaggat gaagatcgtg cggcaccctg
     1201 gtttgtgtgg atgactaaac tcgaatgggc gataggtgcg gtagtgctgc tgatcatcag
     1261 cctgcttgcg actctcgaac ctttttgatg gattacgaaa atgatgaaaa aaagtattct
     1321 ggcgtttctg ttactcacca gttctgcagc ggcgctggct gcaccgcagg tgattaccgt
     1381 cagccgtttt gaagtgggta aagacaaatg ggcgtttaat cgcgaagagg tgatgctgac
     1441 ttgccgaccg ggtaatgctt tgtatgtcat caacccaagt accctcgtgc agtatccttt
     1501 aaacgatgtc gcacagaagg aagttgccag tgggaagact aaagcccaac ccatttcggt
     1561 gattcagatt gatgatccca acaatcccgg cgaaaaaatg agtctggcac cgtttataga
     1621 acgagctgaa aaactctgtt aattacctaa aatagccttt tgatttccaa taaaaaaacc
     1681 gcctcagttc tttcaccaga acgggcggtt tttaacattt aagctgatga ccaccgcgct
     1741 ttttattgac cattttgcac gcaaactgga aaacctggcg tcgtcatcta ttcttaaagg
     1801 gcaaggcaac taagcctgca ttaatgccaa cttttagcgc acggctctct cccaagagcc
     1861 atttccctgg accgaataca ggaatcgtgt tcggtctctt tttatctgtt aaaagccaga
     1921 agcatttcct tcgctgactt tatagtcaac cataacacac actctactgt ctgagtccag
     1981 cgttttttaa cattcttgtt aagattatgt gatctttagc gcgggaggaa aatattgatg
     2041 aaacagcctg cgcccgttta tcagagaatt gcgggtcatc aatggcgaca tatctggctt
     2101 tctggcgata tacacggttg tcttgagcag ttgcgccgca aattatggca ttgtcgtttt
     2161 gatccgtggc gagatttact tatctcagtg ggagacgtta tcgatcgtgg gccgcaaagt
     2221 ttacgttgtc tgcagttact ggaacaacat tgggttcgtg cggtaagagg caatcatgaa
     2281 cagatggcga tggatgcgct ggcatcccag cagatgtctt tgtggttgat gaatggcggc
     2341 gactggttta ttgcgctggc agataatcaa cagaaacaag cgaaaacggc gctggaaaaa
     2401 tgtcagcatt tgccctttat tcttgaagta cacagccgca ccggcaaaca tgttattgct
     2461 catgccgatt atccagatga tgtttatgaa tggcaaaagg acgttgattt gcatcaggtc
     2521 ttgtggagcc gctcgcgatt aagtgaacgc caaaaagggc agggaattac aggtgctgat
     2581 catttctggt ttggtcatac accgttgcgc catcgcgtgg atattggcaa cctgcattat
     2641 attgataccg gcgctgtctt tgggggcgaa ctgactttcg tgcaattgca ataattaaaa
     2701 atcaccatac tcctgtgctg gtcgccagaa accatctata aaatcctcaa tcggaaaaca
     2761 accgccatga cggatccgtt gatcgctcat agaataaaga cactgctgtt ccgtgttgta
     2821 gacatccaca acaatatctt cacaaccgcc atccaggtag caaacaaaaa gtaccagcgc
     2881 gaacatttca tccccgaagt gtggtgccgt accgttaagt ttaggagaga ttttacaacg
     2941 ggggaataac caggacaaat aacccgccat gccgacgggt tctttttgga tcaggcaaga
     3001 cgcataatcc aggcatcaga ttgttgatcg taacgggtac ggtaaaggac agagtgttcg
     3061 ccatttagca gggcagggat actggtttca gcatcccagg catccagttg catacataaa
     3121 tcggggtctg atttacaagg aatggttaac gttcgatcac ccggcgattc accgtgtaat
     3181 tcgccgtcgt cgatttcaaa agcgcctatg cgcacactgg ttttcatctt cgtgctctcc
     3241 atttagctgc cggttgtggt acgaccagtc cgatcgcacc atttttcagc ctagttgagg
     3301 ccgccgaatg tgccagtcaa aaagtgcgct ttttttgtgc gctgtcatgc gttactggta
     3361 aaaagtttcc cgtcccgcac cagttcacgc gggtagctgt ttttcagtcg cgaaccaatc
     3421 cgtttggcta aaccaatcgg ctgatgctgg aaagtaacca acacgtcatc cgccactggc
     3481 gcggcttgcg ggtaaacatc gcgcccgcga taccactcct ccgcttcttg cggtgtcagt
     3541 tcaaaagcgt tctcgttgtc gggggaggca agggcaataa ccgcttcatg ctgccagcga
     3601 taacctttgt tgtgcgtttc ggcaagttta atccccaggc gagaaaatcg gactttaccg
     3661 atcagggctt caatgcctac cggaaacaac cacacttctt tgtcgcgctg ccagagtcgc
     3721 aggtttccat cccagtttaa gccaacactt gcagccgcct gacgaatttg tgcggcttcg
     3781 cgatctttca ccgggctgaa cgggaaattg cccactttgt attttggggt gggtaaggcg
     3841 ggaatggcct gagttttacg cagacgagca acgaagaagc cttcgcagtc gtaaatttgt
     3901 gggaaaacat gcaaaaagcc ttcttcggtc agcgctttgt ttgcaccagg gaagagatcg
     3961 ccaagtggta aaaactctac tgcgtcgggg taagtctctt taagccacag gcaaacggat
     4021 tcgttttctt cccggtttaa ggtacaggtc gagtaaacca gtgtaccgcc agagcgtaat
     4081 gcatgaaagg cactgtcgat cagttcccgt tgggtagctg cgatttcctg attgctttct
     4141 ggtgaccaat tttttagcgc atcgggatct ttacgcacca cgccttcgcc ggagcaggga
     4201 gcgtccagca aaatggcatc gaacatttct ggcaatgccg caccaaacac gcggccatca
     4261 aaatgtgtga gcgcaacatt actgatacca cagcggctga tattggcatg taacactttt
     4321 acccgactgg cggaaaactc attggcaagg attgcccctt cgttattcat ccgcgcggca
     4381 atttgcgtcg ttttggagcc tggcgcagca gcgacatcca tcacccgctg tggtgcatta
     4441 ccgtcagcaa acaaggcggc gacgggcaac attgaactgg cttcctgaat ataaaacagg
     4501 ccacttaaat gctcggcggt actacccaat ggcaatgcat cttcatcgtc gcgttcaatc
     4561 cagaaacctt cttcacacca cggaattggc gtaagcgtcc agccataagg agcggttaat
     4621 cgcaggaaat cagcaacgga ggttttcagc gtattaacgc gaatgctgcg gcgcaacggg
     4681 cgctgacagg cggcaagaaa atcatcaaaa gagagcgtcg aaggcattgc ttcgcgcatt
     4741 tgcgtcagaa aggcgtccgg gaaataaacg gtgtgttggg ccacgagcat ataccactgg
     4801 caaaaaaaac aggcgcgcag tttagcataa acgctccggc gcaggcacgc cggagcagtg
     4861 ggcattattt gggaagcgca gttccccatt cacgccactc tttcggttca ctttcctgca
     4921 acaggaagtg cttgccttcc tgggctttcg gtgccagtgg cgtacccgga ggcgtggcga
     4981 aggcgatgcc gccacggata aactggttaa aggtgccggt tttcactacg ccgcccgtca
     5041 gaccaaagtc cagactgtaa cccgatgcca accagaagac ggaattgtta cgcaccaggt
     5101 gttgatagcg tttactgatg cgcatcgcaa tcatcacgcg atctgacaat gtccccagcg
     5161 tcattccggt aaccgtaccg acttccagac cacggaacag cacaggcgta ccgatgccta
     5221 acgaacaggc ttccggcgct tcaacaataa tgcttaaacc atccaggtaa cgcgaatcag
     5281 taatggtggc ctcttgtaat tcaaagtcgc gacgaggatt gccacggcct ggttcgacgt
     5341 tgatatacgg ctggaggata gtatcaagat gctcaacgcc agctgccgaa atttgcggtg
     5401 tgaccactga gaagcgcgta ccaccgcgag caaaggtctg gacatattcc ggatagagca
     5461 ccgcctttgc ctgtacttca ttgcgcgcgg taatcagatc cagcgtctgg atttgcccga
     5521 tatcaatacc gagatagcga attggcatcc cgaccgccag ttttccggca tcgaaagcat
     5581 gaagcgtaat ctgcccgcca accgcacggg ccgctgtttc ggaagcatac agaatacgct
     5641 tatcgccttt acgctgactg gcgctggcac cgctgaggtt atcgaagcta atggctccct
     5701 ttaatgctct ggagagtggg gatgcctgta cggtcaggcc actaccattc agctgaactt
     5761 tcgccccgcc ttctgcccag aacacgctat tgttggtcag aaggttgcga tactccggct
     5821 taatatgcag atcgatatca aacgcgttag ctcgcggacg cacggtaata acttcaccaa
     5881 cttcaaattt acggtacagc actaccgatc ctgcctgcac atccggcagc gtctctgcac
     5941 tcaaactcac ggttgtggtg ggtaaatcgc taaggctgtt ctccagcgct ttttccagat
     6001 tggcatacag tggatagctg gctttcatct cacctttatc gcccggcaga atacgtatcc
     6061 cgccattgat ccattctgag gcgctggcac cgagaaactc aacgccatcc agccccacct
     6121 tcacatcgac acggctgttg acgacaaatt tgctatcgcc ttttaccagt tcgcgatgct
     6181 gaggctcaat ggcgacggta aaggtaacgc ctttgctggt gagtttacga tcaataacct
     6241 ggcctacctg cacgccgtga agaatgagcg gctgaccagc atcaataccg taactttccg
     6301 gtgctgtcag ggtcagtgtc agaacatcag gttcatgcag cagtgctttt tcgcctggca
     6361 caacaacgaa ctctttgcgt ggctcgccat cgccaggcac caactcaaag gtttttccgg
     6421 tcagcagggc gctgagattg gcatcgctaa gggataattt cgggttgcgt aattcgatgc
     6481 gggtattttc ccgtaacagc gtaacgacgc tgggatcaac ggtcatttct ccggtgactt
     6541 taccaccagg atttaaatcc agtttagtca gctgtccgac ttccagcccc tgatacatta
     6601 acggcgtcga gtcggcggtt aatccggccc cactcggcag ttccagtttt attattacgc
     6661 cacgctggct gtgggccaga tcttcataca gaccaaaggt atcttccgcc tcggcaggtt
     6721 tcgactcttc tggtgaatcg aaggcaatcg caccgttaac cagtgccgcc agactttcca
     6781 gtttcacctt cgcgccactg atactgacgt tggcatcaac gcctgaaacg ttccagaaac
     6841 ggctaccttt tttcaccagg tcggtaaaac gccgctcgat caggacatca atcaccacgc
     6901 cttgtttatt gggattgatg gcatagtcgt agacttttcc caccgggatc ttgcggaaat
     6961 agaccaatga accgctgttc agcgaaccga gatcgggagc ttgcaggtgg atcatcagat
     7021 cgccgttatc cagccgatac ttcggttggg tatcgagcgc gacaaagtga tcctgctctt
     7081 tacctttacc tggcatcatg ccgatatagt taccaccgac gagggcgtcc agcccggaga
     7141 cacctgccaa cgatgctttt ggcgtcacca gccagaactg agtctcttcg cgcagcgcat
     7201 ctttcatatc ggacttgatg ctgaccttga cttcaatctt acgaagatcg tcgctgaggc
     7261 tgatatcctg cactgttccg acttcaacgc cctgataacg aacaggcgta cggcccggga
     7321 caataccatc cgccgacata aagtcgatgg tgacggtatt accccggtcc tgataactgt
     7381 cccaaatcag ccaaccggca atcattagcg cgatgaacgg cagcagccag aaaggtgaga
     7441 tacggcgttt atttttaatc tgcgcttcag tcgtcgaagc gggcgtttcc tgactcatgt
     7501 gcatcccaaa gtaagcggct gtccagccat tccacagcaa gaatagtcaa aattaccgct
     7561 gcgccgaaat aaaacgcagc cggtcccata gtaaaagcga ggatctgatc gcgattaatc
     7621 agcgacatgg ttaaagatat gacaaacagg tcgagcatcg accagcgacc aatccaggtc
     7681 accatccgca gtaacagaat gcgtgtgcgt aaaccttgct ggcatttaaa atgaatgctc
     7741 aacagtaaag tgaacatgac gatcacttta gtaaacggta ccagaatact ggcgataaac
     7801 acgattcctg caaccgcaat gttgctactt gccagcgaca taattccgga aagaattgta
     7861 tcttcctgcc gtcctccgtt cagataaatg atagaaatag gcaacaggtt ggcaggtaac
     7921 aataaaacga ttgacgctaa cagcgccgcc cagcattttt gcagactatg acggcgacgc
     7981 aggcgtagcg gaatatgaca acgcgggcag cgaccacgct gatcaggata gccggtaaaa
     8041 tggcacccaa gacagacacg aagtttctcg tccctacgcg tagcggggcg ctgcggataa
     8101 aatcgctccc acagctcctc gacattaaga tgtgacaacg tcaccgtcgt taaaatcacc
     8161 aacgccacaa aagagaacaa gccgacaccc gcctggatat gggcataatc ctgtaccttt
     8221 atagaagcaa cgccaatgcc gaccaggtag atatcgagca ttacccactc tttaagtcgc
     8281 tcaagcatca gcagtaccgg gcgtagattc attcccagtc ggttaccaaa ccataaataa
     8341 gctatggagg tcaccagaat gagcggggca ccaataacgc agaaaaagac catcgacccc
     8401 gttatcgcat cgccctgttt ggtcatttgc cagatgcctt gcataacgtt ggcgtcaata
     8461 cgaatgccta acagccagat atgcaacagc ggttcgcccc aggcaaacgg catcagcaac
     8521 agcatggtga aggccattgc cgccaggcgc gttagcgacc agtcgcgccc gtcacgaatt
     8581 tttgcctgac agcgcggaca ataggcactt tgatgagaat ttatctcggg caggctaaac
     8641 agcatgtcac attgcgggca acgttggtaa tcaccacgcg gcagttcctc gccgattgcc
     8701 ctcactgtta tcttttttgt cggcgtaatt tgtggtgtgt taagagccat gacgtgccaa
     8761 atgtaataag aattaacatt aatcttaacc catgatggga atgatcttga gcattaacgc
     8821 atttaatgct tattttaata ggctggcagt tccaggtaag acaaccaagt gattatataa
     8881 tgaacaaaac agaattttac gcggatttaa atcgcgactt taacgcgctg atggcgggag
     8941 aaaccagttt tctggcaacg cttgcgaaca ccagtgcgtt gttatatgag cgtctcactg
     9001 acgtaaactg ggcaggtttt tatttgcttg aggacgatac actggtgctc ggaccatttc
     9061 agggcaaaat tgcctgtgtc cggatacccg tcgggcgtgg cgtgtgcggc actgcggttg
     9121 cccgcaatca agtgcagcgt atcgaggatg ttcatgcgtt tgacgggcat attgcctgtg
     9181 atgcggcgag taattctgaa attgttctgc cgctggtggt gaaaaatcag attattggtg
     9241 ttctcgacat cgatagtacc gtcttcggtc gctttacaga cgaggacgag caaggcttac
     9301 gtcagcttgt ggcacagctt gaaaaagtgc ttgcaacgac ggattacaaa aaattctttg
     9361 cgagcgtcgc aggataatca acggataacg tagcatttac tgatagcgtc attataatga
     9421 cgcctgttca tgcctgcgct tgttggctac gtccgttgta atcaggaaat ttcatggaaa
     9481 atcaacctaa gttgaatagc agtaaagaag taatcgcgtt tctggccgaa cgttttcccc
     9541 actgtttcag tgcggaaggt gaagcgcgtc cgctgaaaat cggtattttt caggatttgg
     9601 tcgatcgtgt tgccggggaa atgaacctga gcaaaacgca attacgatcc gctttacgtc
     9661 tctacacttc gagctggcgt tacctttacg gtgttaaacc cggcgcaacg cgtgtcgatc
     9721 ttgacggcaa cccatgcggt gagctggacg agcaacatgt agagcatgct cgcaagcagc
     9781 ttgaagaagc gaaagcgcgt gttcaggcac agcgtgctga acagcaagcg aaaaaacgcg
     9841 aagctgccgc agctgctggt gagaaagaag acgcaccgcg tcgcgaacgc aagccacgtc
     9901 cgaccacgcc acgccgcaaa gaaggcgctg aacgtaaacc tcgtgcgcaa aagccggtag
     9961 agaaagcgcc aaaaacagca aaagcacctc gcgaagaaca gcacacccca gtttctgaca
    10021 tttcagctct gactgtcgga caagccctga aggtgaaagc gggtcaaaac gcgatggatg
    10081 ccaccgtatt agaaatcacc aaagacggcg tccgcgtcca gctgaattcg ggtatgtctt
    10141 tgattgtgcg cgcagaacac ctggtgttct gaaacggagg ccgggccagg catgaacatg
    10201 ttttttaggc ttaccgcgtt agctggcctg cttgcaatag caggccagac cttcgctgta
    10261 gaagatatca cgcgtgctga tcaaattccg gtattaaagg aagagacgca gcatgcgacg
    10321 gtgagtgagc gcgtaacgtc gcgcttcacc cgttctcatt atcgccagtt cgacctcgat
    10381 caggcatttt cggccaaaat ctttgaccgc tacctgaatc tgctcgatta cagccacaac
    10441 gtgctgctgg caagcgatgt tgaacagttc gcgaaaaaga aaaccgagtt aggcgatgaa
    10501 ctgcgttcag gcaaactcga cgttttctac gatctctaca atctggcgca aaagcgtcgt
    10561 tttgaacgtt accaatacgc tttgtcggta ctggaaaagc cgatggattt caccggcaac
    10621 gacacttata accttgaccg cagcaaagcg ccctggccga aaaacgaggc tgagttgaac
    10681 gcgctgtggg acagtaaagt caaattcgac gagttaagcc tgaagctggc aggaaaaacg
    10741 gataaagaaa ttcgtgaaac cctgactcgc cgctacaaat ttgccattcg tcgtctggcg
    10801 caaaccaaca gcgaagatgt tttctcgctg gcaatgacgg cgtttgcgcg tgaaatcgac
    10861 ccgcatacca actatctttc cccgcgtaat accgaacagt tcaacactga aatgagtttg
    10921 tcgctggaag gtattggcgc agtgctgcaa atggatgatg actacaccgt tatcaattcg
    10981 atggtggcag gtggtccggc agcgaagagt aaagctatca gcgttggtga caaaattgtc
    11041 ggtgttggtc aaacaggcaa gccgatggtt gacgtgattg gctggcgtct tgatgatgtg
    11101 gttgccttaa ttaaagggcc gaagggcagt aaagttcgtc tggaaatttt acctgctggt
    11161 aaagggacca agacccgcac tgtaacattg acccgtgaac gtattcgtct cgaagaccgc
    11221 gcggttaaaa tgtcggtgaa gaccgtcggt aaagagaaag tcggcgtgct ggatattcct
    11281 ggcttctatg tgggtttgac agacgatgtc aaagtgcaac tgcagaaact ggaaaaacag
    11341 aatgtcagca gtgtgatcat cgatctgcgt agcaatggtg gtggggcgct gaccgaagcg
    11401 gtatcgctct caggtctgtt tattcctgcg ggtcctattg ttcaggtccg tgataacaac
    11461 ggtaaagttc gtgaagacag cgataccgac ggacaggttt tctataaagg cccgctggtg
    11521 gtactggttg accgcttcag tgcttcggct tcagaaatct ttgccgcggc aatgcaggat
    11581 tacggtcgtg cactggttgt gggtgaaccg acgtttggta aaggcaccgt tcagcaatac
    11641 cgttcattga accgtattta cgatcagatg ttacgtcctg aatggccagc gctgggttct
    11701 gtgcagtaca cgatccagaa attctatcgc gttaacggcg gcagtacgca acgtaaaggc
    11761 gtaacgccag acatcatcat gccgacgggt aatgaagaaa cggaaacggg tgagaaattc
    11821 gaagataacg cgctgccgtg ggatagcatt gatgccgcga cttatgtgaa atcaggcgat
    11881 ttaacggcct ttgaaccgga gctactgaag gaacataatg cgcgtatcgc gaaagatcct
    11941 gagttccaga acatcatgaa ggatatcgcg cgcttcaacg ctatgaagga caagcgcaat
    12001 atcgtttctc tgaattacgc tgtgcgtgag aaagagaata atgaagatga tgcgacgcgt
    12061 ttggcgcgtt tgaacgaacg ctttaaacgc gaaggtaaac cggagttgaa gaaactggat
    12121 gatctaccga aagattacca ggagccggat ccttatctgg atgagacggt gaatatcgca
    12181 ctcgatctgg cgaagcttga aaaagccaga cccgcggaac aacccgcacc cgtcaagtaa
    12241 tatcaatcag gcacaagaaa ttgtgcctga ttttttaaca gcggcaagat gccgtaaatc
    12301 agatgctaca aaatgtaaag ttgtgtcttt ctggtgactt acgcactatc cagacttgaa
    12361 aatagtcgcg taacccatac gatgtgggta tcgcatattg cgttttgtta aactgaggta
    12421 aaaagaaaat tatgatgcga atcgcgctct tcctgctaac gaacctggcc gtaatggtcg
    12481 ttttcgggct ggtactgagc ctgacaggga tacagtcgag cagcgttcag gggctgatga
    12541 tcatggcctt gctgttcggt tttggtggtt ccttcgtttc gcttctgatg tccaaatgga
    12601 tggcattacg atctgttggc ggggaagtga tcgagcaacc gcgtaacgaa agggaacgtt
    12661 ggctggtcaa tactgtagca acccaggctc gtcaggcggg gatcgctatg ccgcaagtgg
    12721 ctatctacca tgcgccggac atcaacgctt ttgcaaccgg tgcgcgccgt gatgcctctc
    12781 tggttgctgt cagcaccggt ttgctgcaga acatgagccc ggatgaagcc gaggcggtaa
    12841 ttgctcacga aatcagccac atcgccaatg gtgatatggt caccatgacg ctgattcagg
    12901 gcgtggtgaa caccttcgtt atctttattt cccgtatcct ggcgcagctt gccgcgggtt
    12961 ttatgggcgg aaatcgtgat gaaggtgaag agagcaacgg caacccgctg atctactttg
    13021 cggttgcaac ggttctggaa ctggtgtttg gtattctggc gagcattatc accatgtggt
    13081 tctcgcgtca tcgtgaattc catgctgatg ccggttcggc aaaactggtt ggtcgcgaga
    13141 aaatgattgc cgcgctgcag cgcctgaaaa ccagctatga accgcaagaa gcaaccagca
    13201 tgatggcttt ctgcattaac ggtaagtcga aatcgctcag tgagttgttt atgactcacc
    13261 cgccgctgga taaacgtatt gaagctctgc gtacgggtga atacctgaag taatatgaag
    13321 taaatactaa aaagcgcgtc ctgctcgacg cgcttttttt atgccctgga tcttggctga
    13381 gtgatacgta acccactgat acaggcagca atcactgcca gaatagccgc agccatcagc
    13441 gagacgtgtg taccattatc tccaaactga tttagcatca gcgccaccag cgccgcgccg
    13501 ctactctgac ccagtagacg agccgttcct aacatgccac tggctccacc gctacgttcg
    13561 cgaggcgcgg aggtaataat ggtgtggtta ttgggtgact ggaacaagcc aaatccagcg
    13621 ccacataaga tcatcggcca gataatattg atatccgcag gtgacgcggg cagcagaacc
    13681 agggaaaaaa gccccgcagc catgatgaat aatcctaaag cacccagcaa tcctgcatgt
    13741 acgcgttcaa tcaaatagcc tgccaacgga gccatcacca tcgttgctaa cggccacggt
    13801 gtcagaagta aacctgtttc gacttcacta cgcccgagca cggtttgcag gtaaaagggc
    13861 agggaaacca ttgccagcat ttgtgcgcag aaagagcaaa cagatgtgca aatagaaagt
    13921 gaaaacagcg ggatacgcag taaatccacc ggtagcagcg gtactggaag agaaagctgg
    13981 cggcgaataa agaaaatacc aacgacaacc attacaacca gttccgcagc aattaacgtc
    14041 agcgattgcc cctgagcgaa accactcaac gcagtgataa gcaggccgaa ggttaacgcg
    14101 ttcatcacgg cgctgggcag gtcgaaacgg ggtttactgg cgcgagaacc attaggtggc
    14161 agaaaacgca tcgccagaag cagggcgata atacccaacg gtacgttgat taaaaataac
    14221 catttccagg atgcgatgga gaggattgct gcagcaattg tcggcccggc agcagaagag
    14281 acggcaacaa taaacgagtt tatgcccatc cctctaccca gaaaacgttg tggatagatc
    14341 aggcggataa gggcggtatt aacgctcatc aacgctgcac cgccgaaacc ttgtatgaca
    14401 cgcgcaaggg tgagtatttg cagcgaatca gaaagggcgc agaacagtga agacaacaga
    14461 aaaacgacca gaccgcattt ataaatacgt cgatagccaa acatatcacc cagaaacgaa
    14521 aacgagagca gggagatgac aatggcgatt tgataggcgt tcactaccca gatggaactg
    14581 gctggcgtgg catgaaggtc cgtggcgatt gttggcaggg cgacgttggc gattgcgccg
    14641 tcaaggacag ccatcgaaat accaatcaca atggttaata tcgcaccgta tcgctggggc
    14701 aatggcaggc cgtcggcctg aacttttggc ataggaattt taaatctttg gtgaataatc
    14761 aacaggggta tgattctagc actgatattc acggtggatg tcgcagaatt gtaactaacc
    14821 ggcaaaagat ggattgcgac caccctggtg cgaaattata ataaaaaaca gttctgattt
    14881 ttataaaaca ctcgcaatga ggtgataaat ggctaacgca gatctggata aacagcctga
    14941 ttctgtatct tccgtgctaa aagtttttgg cattttgcag gcgctgggtg aagagcgcga
    15001 aatagggata accgagctgt cgcagcgcgt catgatgtca aaaagcaccg tttatcgctt
    15061 tttacagacc atgaaaacct taggttatgt ggcgcaggaa ggggagtcgg agaaatattc
    15121 cctgaccctg aaattgtttg aactgggcgc tcgcgcgtta caaaacgtcg atttaattcg
    15181 tagcgcagat atccagatgc gtgagatctc ccgcctgacc aaagaaacta tccacctcgg
    15241 cgcactggac gaagacagta ttgtttacat tcacaaaatt gactctatgt acaatttgcg
    15301 catgtattca cggattgggc gtcgtaatcc actgtacagc accgcgattg gtaaggtact
    15361 gctggcatgg cgcgatcgcg atgaagtgaa gcaaattctt gagggcgtgg agtataaacg
    15421 cagtaccgag cggaccatca ccagtacaga agcgttatta ccggttctgg accaggtgcg
    15481 cgagcagggg tatggcgaag ataacgaaga gcaggaagaa gggctgcgat gcattgcggt
    15541 tccggtattt gatcgctttg gcgtggtcat tgccggtttg agcatctcct tcccgacgtt
    15601 gcgtttctct gaagagcgtt tacaggaata tgtcgcaatg ttgcataccg cagcgcgcaa
    15661 aatttctgcc caaatgggtt atcacgacta tccgttctga tgagagtaag aacctgtcgg
    15721 aatatcaaac agacaggttc tttatttagc atgagaaaaa taaagttgaa ggtggcgtta
    15781 gattaaacgc agttgatata agaatatttt actcatgagt gagaatctgg ttatttatta
    15841 cacttaacca ttatcgacca cgatattgct tttgcgtaac agcgggcaat ctgttatccc
    15901 caaaaaacca cttttagtgt gcaagtattg taccgtgctg gtgcctctgg cagtcagata
    15961 ggtacattgc aaacctaatt ctgcggcatt ctctttgctt ccaatcaaaa cgccatatcc
    16021 gctgagtaat aatcctatcc ataccagtgc tatcagcata actgtgcgaa tgatgaatcg
    16081 cattacaacc tcttctcttt tttatgttcg cttaatcgta gcggcaatat gcgctgaagc
    16141 aagcgactca ttccgaaaaa gcacgaatat cgacatagtt agacgctgtt taactaacgc
    16201 atggtagttt aatgacataa ggtaggtgaa acggagattg gagtgaaaaa gtttcgatgg
    16261 gtcgttttgg ttgtcgtggt gttggcttgc ttgctgcttt gggcgcaggt attcaacatg
    16321 atgtgcgatc aggatgtaca atttttcagc ggaatttgtg ccattaacca gtttatcccg
    16381 tggtgatatc attttttcag cactagattt tcttcctctc cagcagtggt agaatagcgc
    16441 ctctactcag aggtggtgaa atgaacgaag ttgtaaattc aggcgtgatg aacattgcgt
    16501 ctttggttgt atcggtggtg gttcttctta tcgggctcat cttgtggttt tttattaatc
    16561 gtgccagttc tcggactaac gaacagattg aactgcttga ggcgttgctg gatcagcaaa
    16621 aacgtcaaaa tgcactgtta cgtcgtttgt gcgaagcaaa cgaaccagag aaagcagata
    16681 aaaagactat tgagagtcaa aaatcggttg aagacgaaga tattattcgc ctggtcgccg
    16741 aacgataatc cttcctcaat cttgagtaac cccgtcgttg aagggttact caattcatcc
    16801 tgattatacg cactgtcatg gtgcaactgc ttcaaattaa a
//
LOCUS       DBFIFM010000099        16836 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.099, whole genome shotgun sequence.
ACCESSION   DBFIFM010000099 DBFIFM010000000
VERSION     DBFIFM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.099"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            283..528
                     /locus_tag="N7C74_002733"
     CDS             283..528
                     /locus_tag="N7C74_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HGU2432573.1"
                     /translation="MCDTKRSIGRKCESGLAANVPLRGVFVQDYDSHTQPKLTNRRIQ
                     MDAQTRRRERRAEKQAQWKADRILASTFRRNATKRHY"
     gene            473..1393
                     /locus_tag="N7C74_002734"
                     /pseudo
     CDS             473..1393
                     /locus_tag="N7C74_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(1408..1483)
                     /locus_tag="N7C74_002735"
     tRNA            complement(1408..1483)
                     /locus_tag="N7C74_002735"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1448..1450),aa:Thr,seq:cgt)
     gene            complement(1598..2851)
                     /gene="proA"
                     /locus_tag="N7C74_002736"
     CDS             complement(1598..2851)
                     /gene="proA"
                     /locus_tag="N7C74_002736"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HGU2432574.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVVVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIAYSFLPALSKQ
                     MAESGVTLHADASALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(2863..3966)
                     /gene="proB"
                     /locus_tag="N7C74_002737"
     CDS             complement(2863..3966)
                     /gene="proB"
                     /locus_tag="N7C74_002737"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HGU2432575.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            4254..5309
                     /gene="phoE"
                     /locus_tag="N7C74_002738"
     CDS             4254..5309
                     /gene="phoE"
                     /locus_tag="N7C74_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HGU2432576.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(5348..5749)
                     /gene="crl"
                     /locus_tag="N7C74_002739"
     CDS             complement(5348..5749)
                     /gene="crl"
                     /locus_tag="N7C74_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HGU2432577.1"
                     /translation="MTLPSGHPKSRLIKKFTVLGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5807..7051)
                     /gene="frsA"
                     /locus_tag="N7C74_002740"
     CDS             complement(5807..7051)
                     /gene="frsA"
                     /locus_tag="N7C74_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HGU2432578.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSCLLHQRVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(7143..7601)
                     /gene="gpt"
                     /locus_tag="N7C74_002741"
     CDS             complement(7143..7601)
                     /gene="gpt"
                     /locus_tag="N7C74_002741"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGU2432579.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            7862..9319
                     /gene="pepD"
                     /locus_tag="N7C74_002742"
     CDS             7862..9319
                     /gene="pepD"
                     /locus_tag="N7C74_002742"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HGU2432580.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(9376..9897)
                     /gene="prfH"
                     /locus_tag="N7C74_002743"
                     /pseudo
     CDS             complement(9376..9897)
                     /gene="prfH"
                     /locus_tag="N7C74_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(9896..10099)
                     /locus_tag="N7C74_002744"
                     /pseudo
     CDS             complement(9896..10099)
                     /locus_tag="N7C74_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(10418..10870)
                     /gene="yafP"
                     /locus_tag="N7C74_002745"
     CDS             complement(10418..10870)
                     /gene="yafP"
                     /locus_tag="N7C74_002745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU2432581.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVMMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPWIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFSMRYKPQH"
     gene            complement(10880..11278)
                     /gene="yafO"
                     /locus_tag="N7C74_002746"
     CDS             complement(10880..11278)
                     /gene="yafO"
                     /locus_tag="N7C74_002746"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HGU2432582.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDESHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(11281..11574)
                     /gene="yafN"
                     /locus_tag="N7C74_002747"
     CDS             complement(11281..11574)
                     /gene="yafN"
                     /locus_tag="N7C74_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HGU2432583.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEKYLNDMTDDDFNDFKE"
     gene            complement(11626..12681)
                     /gene="dinB"
                     /locus_tag="N7C74_002748"
     CDS             complement(11626..12681)
                     /gene="dinB"
                     /locus_tag="N7C74_002748"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HGU2432584.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVSAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(12752..13537)
                     /gene="lafU"
                     /locus_tag="N7C74_002749"
     CDS             complement(12752..13537)
                     /gene="lafU"
                     /locus_tag="N7C74_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HGU2432585.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            13509..15221
                     /locus_tag="N7C74_002750"
                     /pseudo
     CDS             13509..15221
                     /locus_tag="N7C74_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(15366..15863)
                     /gene="rayT"
                     /locus_tag="N7C74_002751"
                     /pseudo
     CDS             complement(15366..15863)
                     /gene="rayT"
                     /locus_tag="N7C74_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414763.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
     gene            complement(16039..16758)
                     /gene="yafL"
                     /locus_tag="N7C74_002752"
     CDS             complement(16039..16758)
                     /gene="yafL"
                     /locus_tag="N7C74_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU2432586.1"
                     /translation="MSGLLLICLPFSSFASATTSHISFSYAARQRMQNRARLLKQYQT
                     HLKKQVSYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLSS
                     DHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILEAKLPRTANEM
                     YHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSRLA
                     EPFWQDHFLGARRILTEETIL"
BASE COUNT         4219 a         4346 c         4225 g         4046 t
ORIGIN      
        1 accaattgta tttattttaa aattaatagg tgcaactcac taaacaacgc aattctgatc
       61 tctcgatcac ctcccaagcc acacaaccct gcaaaaaata aatctatata aaaaacatac
      121 agataaccat ctgcggtgat aaattatctc tggcggtgtt gacacaaata ccactagcgg
      181 tgatactaag cacatcagca ggacgcacta accaccatga aggtgatgct cttaaaaatt
      241 aagccctgaa gaagggcagc attcaaagca gaaggctttg gtgtgtgtga tacgaaacga
      301 agcattggcc ggaagtgcga atccggatta gcagccaatg tgccattgcg gggtgttttc
      361 gttcaggact acgactccca cacacaacca aagctaacta acaggagaat ccagatggat
      421 gcacaaacac gccgccgcga acgtcgcgca gagaaacagg ctcaatggaa agcagatcgg
      481 attctggctt caacatttcg caggaatgca actaagagac attactgaat caaaaattta
      541 ttcagcaatg cagaaaatga cgaaccggcg tcatgaggaa aactggaaac tcagggcaga
      601 agcatgcaga aaaaaaggga aacctgttcc agaatacacg ccaaaaccag cgtccgttgc
      661 aacgaaggct acgcatcttt catttataaa ggccctgcta agagccgcag agcgtgaatg
      721 gaaaatgctc gataaggcac caattattaa agtgcctcaa ccaaagaata aacggatccg
      781 ctggctggag ccccatgaag cacaaaggct gattgatgaa tgtccggagc cattaaagtc
      841 tgttgttgaa tttgcactgg caacaggctt aagacgctcg aacatcatca accttgaatg
      901 gcaacaaata gatatgcagc gccgggtggc atggataaac ccggaagaga gtaaatcaaa
      961 ccgcgcaatt ggcgttgcgc tgaatgatac tgcatgtcgc gtattgaaaa aacaaatcgg
     1021 taatcatcac cgttgggtat ttgtgtacaa ggaaagctgt accaaaccag acggaacgaa
     1081 agcgccaaca gtaaggaaga tgcggtatga cgcaaacaca gcctggaaag cggcgctgag
     1141 acgggctggt attgatgatt tcagatttca cgacttgaga cacacctggg caagttggct
     1201 ggttcaagcc ggagtcccgt tgtcagtgtt acaggaaatg ggaggctggg agtctatcga
     1261 aatggttcgt cgatatgctc accttgcacc taatcacctt accgaacacg cacggcaaat
     1321 agactcgatc ctgaacccat cggtcccaaa tttgtcccag tcaaaaaata aggaaggtac
     1381 taatgatgtg taacttattg atttaaatgg tgccgataat aggagtcgaa cctacgacct
     1441 tcgcattacg aatgcgctgc tctaccaact gagctatatc ggccctgaaa ggacatgttc
     1501 acgaacgtga atcacggtgg acaaggttaa aactaaccgg gcgatgcgtc aatggccttg
     1561 tgaatcaaac ggctactttt gcatcacccg gttttattta cgcacgaatg gtgtaatcac
     1621 caatgccgat ccacttgtaa gtggtcagtg cttccagccc cattgggcca cgcgcgtgga
     1681 gtttttgtgt gcttaccgcc acttccgcac ccagtccaaa ctggccgccg tcggtaaaac
     1741 gcgtagaggc gttaacgtaa acagcggacg aatccacttc gttaacaaaa cgctgggcgt
     1801 tgcgcatatc gcgggtcagg atcgcatcgg agtgttgcgt gccgtgttca cgaatatggg
     1861 cgatggcatc gtcaagatcg ctgacgattt tgacgttcaa atctaatgac agaaactcat
     1921 cgtcatactc ttcggcttta accgccacca ccttcgcagg gcctgtctgc aactgcgcca
     1981 gcgcagatgc atctgcgtgt aacgtcacgc cgctttccgc catttgtttg cttaatgcgg
     2041 gcaggaagct ataggcgatg tttttattca ccagcaacgt ttctaccgta ttacatgtgc
     2101 tcggacgctg agttttcgca ttgacgatta ctttcagggc ttcagcaatc tctgcacttt
     2161 catcaacata aatatggcat acgcctatac cacctgtgat caccgggatc gtcgactgct
     2221 cgcggcacag tttatgcaaa ccagccccgc cgcgcgggat cagcatgtcg atgtatttat
     2281 ccatacgcag catttcactg accagcgcac ggtcaggatt atcaatcgcc tgcacggcac
     2341 ccgccggtaa gccacaggat ttcagggcgt cctgaatcac caccaccgtt gccgcgttag
     2401 tgcgacaggt ttctttgcca ccacgcagga tcaccgcgtt accggttttc aggcacagcg
     2461 aagcgacatc aaccgtcacg ttcgggcgcg cttcataaat cacgccaata acccccagcg
     2521 gtacgcgacg acgctcaaga cgcaggccgc tgtccagtac gccgccatcg attacctgcc
     2581 ccactggatc ggcgaggttg cacacctgac gtacatcgtc ggcaatgcct ttcagccgtg
     2641 cgggcgtcag tgccagacgg tcaagcatcg cttcgctaag gccattggct cgcgcgtcag
     2701 caacatcctg ggcgttagcg ttgaggatga tttcgctttg tgcttccagt tcatcggcga
     2761 ttttttccag cacgcgattt ttttcgcggc tggagagttg cgctaattta tacgaggctt
     2821 gcttcgcggc aatgcccatt tgttccagca tcagcctgct ccttaacggg tgatcatgtc
     2881 atcacggtga acggcaaccg ggccgtattc atatcccagt attgcatcaa tttcttgcga
     2941 gtggtgtccg gcaatacggc gtaatgcatc gctgttgtaa cgactgacgc cgtgggcgat
     3001 atcgcgacct tcgaggttac aaatgcggat gacttcacca cgcgagaagt tgccagtcac
     3061 gcttttaatg cctttcggca acagggagct gccgcgttca agaatggcgg cggttgcccc
     3121 ttcatctacc gtgatttcac ccgcaggcgg cgcaccgaaa atccagcgtt tacggttttc
     3181 aagcggagtc gcctgggcat ggaacagcgt accgacggaa atgccttcca tcacatcacc
     3241 aataacgccc ggcttgctgc ccgcggcaat aatggtgtcg atacccgcac ggcaagccac
     3301 gtcagcggcc tgcaatttgg tactcatgcc gccagttccg aggcctgaaa cgctgtcacc
     3361 agcaatcgcg cgcagtgcgt catcaatgcc gtaaacatct ttaatcagtt ctgcctgcgg
     3421 attgctgcgc gggtcagcgg tatacaaacc tttttgatcg gtcagtaaca acagtttatc
     3481 ggcacccgcc agaatcgccg ccagtgcaga aaggttgtcg ttatcgccga ctttaatctc
     3541 tgccgtagcg acagcatcgt tctcattgat taccggaacg atattgttat cgagcaacgc
     3601 acgcagggtg tcgcgggcgt tcaggaagcg ttcacggtct tccatatcag cacgagtcag
     3661 cagcatttgc ccgacgtgaa tgccataaat cgaaaacagc tgttcccaca gttgaatcag
     3721 tcgactctgc cctaccgccg ccagcagttg tttcgaggcg atagtcgctg gcagttccgg
     3781 gtaacccagg tgctcacgtc cggcggcgat cgcgcccgac gtcacaataa caatccgatg
     3841 cccggcggca tgtaactgcg cgcactggcg aacaagttca acgatatggg cacggttcag
     3901 gcggcgcgat ccgcctgtta gcacactggt gccgagtttt accaccagcg tctggctgtc
     3961 actcatgatt ctctgccatt caattttagg aaaaatgata tcaaacgaac gttttagcag
     4021 gactgtcgtc ggttgccaac catctgcgag caaagcatgg cgttttgttg cgcgggatca
     4081 gcaagcctag cggcagttgt ttacgctttt attacagatt taataaatta ccacatttta
     4141 agaatatcat taatctgtaa tatatcttta acaatctcag gttaaaaact ttcctgtttt
     4201 caacggggct ctcccgcaga atattcgcgc gttaattaaa atcaggaatg aaaatgaaaa
     4261 agagcactct ggcattagtg gtgatgggca ttgtggcatc tgcatccgta caggccgcag
     4321 aaatatataa caaagacggt aataaactgg atgtctatgg caaagttaaa gccatgcatt
     4381 atatgagtga taacgacagt aaagatggcg accagagtta tatccgtttt ggttttaaag
     4441 gcgaaacaca aattaacgat caactgactg gttatggtcg ttgggaagcg gagtttgccg
     4501 gaaataaagc ggagagtgat actgcacagc aaaaaacgcg tctcgctttt gccggattga
     4561 agtataaaga tttgggttct ttcgactatg gccgtaacct gggcgcgttg tatgacgtgg
     4621 aagcctggac cgatatgttc ccggaatttg gtggcgactc ctcggcgcag accgacaact
     4681 ttatgaccaa acgcgccagc ggtctggcga cgtatcggaa caccgacttc ttcggcgtta
     4741 tcgatggcct gaacttaacc ctgcaatatc aagggaaaaa cgaaaaccgc gacgttaaaa
     4801 agcaaaacgg cgatggcttc ggcacgtcat tgacatatga ctttggcggc agcgatttcg
     4861 ccattagtgg ggcctatacc aactcagatc gcaccaacga gcagaacctg caaagccgtg
     4921 gcacaggcaa gcgtgcagaa gcttgggcta caggtctgaa atacgatgcc aataatattt
     4981 atctggcaac tttttattct gaaacacgca aaatgacgcc aataactggc ggctttgcca
     5041 ataagacaca gaactttgaa gcggtcgctc aataccagtt tgactttggt ctgcgtccat
     5101 cgctgggtta tgtcttatcg aaagggaaag atattgaagg tatcggtgat gaagatctgg
     5161 tcaattatat cgatgtcggg gctacatatt atttcaacaa aaatatgtca gcgtttgttg
     5221 attataaaat caaccaactg gatagcgata acaaattgaa tattaataat gatgatattg
     5281 tcgcggttgg catgacctat cagttttaat gattattgcc ggatgtgatg catccggcac
     5341 atttcactca cgccgttaac ttcaccggct cgtcacgaaa atcatccgcc ggttccaact
     5401 tcagattcag cgtcgtcagc agttcacgca gcttctcgtg aaactcacgc agggtgtgct
     5461 ccagtcgttc aaccacttca gtatctttta ccggaacact cttccagtcg cctgctttat
     5521 cgaacagacc aaactggtaa ctgtaggtaa aacgggattc ctgcgcttca agctccatcc
     5581 accagcccca gaactcacgc acttccggtg ccggtttcac gttgacgcat acagccagac
     5641 aatcgaaaaa gaatcgatta tcttcgcact taccttcacg aatatacggg cctagtacgg
     5701 taaatttttt gatcaatctg ctcttcgggt gtccactcgg taacgtcatt gctatctcct
     5761 gttgtgatgc aactgtttta ccaaattggc aaaatttagc aaatttttaa cacaagcgtt
     5821 tttcgatcca gtcggtgatt tcctgaagac ctttgtcaaa attccgatac accgggttaa
     5881 atgggatctc taataattta ccgtcagcag atgatgaggt gattaagcgt gagtcctctt
     5941 ccgggctgaa cggatcgttc ttccagtagc ctgataacat tggcgttggg catcgacgtc
     6001 ccagcaatcc ttgcactttt aatgaatagc gattcagctc cacgcgcaac gcttcgtcgg
     6061 aagcatcatg catccccaaa cgactcgcca gaacgtcaag atacatttcc ggcacctgtt
     6121 gctggcactt aaaatcactc aacagggtat gaactaccgg accaagacag gcaaccgctt
     6181 tcagacgcgg cgattcaaga tatgccagac gcacggcaac gttagcgccg aaacggaaac
     6241 caaaggccgc gacgcgagtg tgatccaccc acggtacgtt aggcagagcc tttaagacgc
     6301 gctgatgcaa caggcaggag tcctgggtga gcttccattt tgaagaaaag cccaccgacg
     6361 gcatatcaat agtcagcatc gcaatgccgc gcggcgcaaa ataacgttca tacaggctgt
     6421 aatagtccgt ctgcatcgca tccagaccac cacacattaa taccgtcggg aacgggccat
     6481 cgcctttcgg catatgcaaa aagccggtga tgggcgcacc gccgggtacg gtaaactcca
     6541 tctgtcgcat cgtgcccggt agacgctgag cggcctcttc ataggcgcgg tttgacaaag
     6601 cctgcgcttg ctcggccagg tcatctcctt tcagatgagg ataggcggca atgttgtaca
     6661 acgtagccgc atgcagccag tgacgaccac tgagttgcgg atcttcttcc gcacaggctt
     6721 tttgttgcca caccatcgcc tgggtggccc actcataaat ccagttgccg ccacggtagc
     6781 caatcaccgt atcgtataaa tcatcgtcgg tacgttcggc atcgctcatc acaatacgtg
     6841 cctggacgtc gaggatttcg cgtgggtcaa tgccgcgcca gatccacatc agacggttaa
     6901 tcatgcgata ccagtgaggg atggttttac catcaagggc cgactgcaca ggcggttgtg
     6961 cgccgtgatt aaagcggcgg actagcgtcg aggtttcagg atgtttaaag cggggtttga
     7021 acagggtttc gctcaggttt gcctgtgtca tggatgcagc ctccagaata cttactggaa
     7081 actattgtaa ctcgcctgaa gttaaaaaga acaacgcccg gcagtgccag gcgttgaaaa
     7141 gattagcgac cggagattgg cgggacgaat acgacgccca tatcccacgg ctgttcgatc
     7201 caggtatctt gcgggatatc aacaacataa tcatcaacca gcggacgacc agccggtttt
     7261 gcaaagatgg tgacaaagtg cgcttttgga tacatttcac gaatcgcaac cgcagtacca
     7321 ccggtatcca ccaggtcatc aataacgatg aagccttcgc catcgccttc tgcgcgtttc
     7381 agcactttaa gctcgcgctg gttgtcgtga tcgtagctgg aaatacaaac ggtatcgaca
     7441 tgacgaatac ccagttcacg cgccagtaac gcacccggta ccagaccgcc acggcttacg
     7501 gcaataatgc ctttccattg ttcagaaggc atcagtcggc ttgcgagttt acgtgcatgg
     7561 atctgcaaca tgtcccaggt gacgatgtat ttttcgctca tgtgaagtgt cccagcctgt
     7621 ttatctacgg cttaaaaagt gttcgagggg aaaataggtt gcgcgagatt atagagatct
     7681 ggcgcactaa aaaccagtat ttcacatgag tccgcgtctt tttacgcact gcctctccct
     7741 gacgcgggat aaagtggtat tctcaaacat atctcgcaag cctgtcttgt gttgacaaca
     7801 ttttctgcta accctgtgac ctgcaatact gttttgcggg tgatcgacaa ggagacttaa
     7861 cgtgtctgaa ctgtctcaat tatctccaca gccgctgtgg gatatttttg ccaaaatctg
     7921 ttctattcct cacccgtcct atcatgaaga gcaactcgct gaatacattg ttggttgggc
     7981 aaaagagaaa ggtttccatg tcgaacgcga tcaggtaggt aatatcctga ttcgtaaacc
     8041 tgctaccgca ggtatggaaa atcgtaaacc ggtcgtcttg caggcccacc tcgatatggt
     8101 gccgcagaaa aataacgaca ccgtgcatga cttcacgaaa gatcctatcc agccatatat
     8161 tgatggcgaa tgggttaaag cgcgcggcac cacgctgggt gcggataacg gcattggtat
     8221 ggcctctgca ctggcggttc tggctgacga aaacgtggtt cacggcccgc tggaagtgct
     8281 gctgaccatg accgaagaag ccggtatgga cggtgcgttc ggcttacaga gcaactggtt
     8341 gcaggctgat attctgatta acaccgactc cgaagaagaa ggtgaaatct acatgggttg
     8401 cgcggggggt atcgacttca cctccaacct gcatttagat cgtgaagcgg ttccagctgg
     8461 ttttgaaacc ttcaagttaa ccttaaaagg tctgaaaggc ggtcactccg gcggggaaat
     8521 ccacgttggc ctgggtaatg ccaacaaact gctggtgcgc ttcctggcgg gtcatgcaga
     8581 agaactggac ctgcgtctta ttgatttcaa cggcggcaca ctgcgtaacg ccatcccgcg
     8641 tgaagccttt gcgaccattg ctgtcgcagc tgataaagtc gacgccctga aatctctggt
     8701 gaatacctat caggagatcc tgaaaaacga gctggcagag aaagagaaaa atctggcctt
     8761 gttgctggac tctgtagcga acgataaagc tgccctgatt gcgaaatctc gcgatacctt
     8821 tattcgtctg ctgaacgcca ccccgaacgg tgtgatccgc aactccgacg tggcaaaagg
     8881 tgtggtcgaa acctccctga acgtcggtgt ggtgaccatg actgacaata acgtagaaat
     8941 tcactgtctg atccgttctc tgatcgacag cggtaaagac tacgtggtga gcatgctgga
     9001 ttcgctgggt aaactggctg gcgcgaaaac cgaagcgaaa ggcgcatatc ctggctggca
     9061 gccggacgct aattctccgg tgatgcatct ggtacgtgaa acctatcagc gcctgttcaa
     9121 caagacgccg aacatccaga ttatccacgc gggcctggaa tgtggtctgt tcaaaaaacc
     9181 gtatccggaa atggacatgg tttctatcgg gccaacaatc accggtccac actctccgga
     9241 tgagcaagtt cacatcgaaa gcgtaggtca ttactggaca ctgctgactg aactgctgaa
     9301 agaaattccg gcgaagtaat tatttgattt gctgccggat ggcgtttaat cgccttccgg
     9361 cagtttcatc cttcattatc cttcgataaa agccatccct gtgaacgttc gtcgcgggtt
     9421 gccacgttca atctggtgat ggaacattcg ccgctgcgat ttcagcactg cgctattttc
     9481 ctgttgctgc tgctccagct tccaggcaat cagcaatcgc gccagccgtt tgttagcatg
     9541 ctggctgcgt tctgactgaa ccttcacgct aatcccggtt gccagatgcg tggcgcgtac
     9601 cgccgagtcg gttttattga catgttgacc gcccggcccc gacggacgca gcgtctcata
     9661 acggattgca tccgattgtt cctgctcatc agcggtaaaa cgcccaatgc ccagaaacca
     9721 gtttttgcgc ccatgatgag gccgatacgg actcagacaa atccactgaa tagtgccgca
     9781 ccaactttcg cttaacgccc atgcgttgtc gccatccaga gaaatcagcg ccgaacgcag
     9841 cgtgtcagag tagcggcccg tttctgtttc cagcaccgtt accgcgacgt cttgtcgggt
     9901 aaaaacgacc aacagagaag aacgctgcgc caatcgggta gccgcgtccg gggtgtaaat
     9961 ctggcatccc cactacgcgc tgcatgttgg gtaaatttgc cgtggtgtgt aattgttgga
    10021 tcgctaaact ctcgatccac aggtcatcag atgcgatggt aaataccgca tcggataagg
    10081 gacgaatata tttgcccatg taccattcca tttatactcg tcatacttca agttgcatgt
    10141 actgcgactg cgttcgttca ccccagtcac ttacttatgt aagctcctgg ggattcactc
    10201 gcttgtcgcc ttcctgcaac tcgaattatt tagagtatat aaatataaaa atcaggaaat
    10261 ggcagacgaa tccagggcaa gaaaaaaaag aattaatcct ggaaaggtct gcaaagagtc
    10321 attgataaag gatttgtcat gatttacctc cttgcagttt gtggaataag aaaacgcgca
    10381 cataccaggc gggcgttatt ttcattgcaa gctggattta atgttgcggt ttatatcgca
    10441 tagaaaaatt agtaaaccac gctccccggc attcaacgcg ctgctgctta actgtctgaa
    10501 aaccataacg ttcaaaaaag ggttttgcgg ttatgctggc gtccaccgta agttcggatt
    10561 cagacttaat ccaaggtttt aacaaagcgc tggcaacccc acggcgggtg tattcagggt
    10621 caacaaataa catatcgata taatgttcaa tgcgggaaat aaaaccaacc ggttgtgcat
    10681 taatgactgc aacccgcact tgtgatttcg cgagtttctc cttccagcga gattcgtcaa
    10741 tctgcgccca ggcggcaatt tgttgtggtg aataatgctg actggcggtc atcataaccg
    10801 ctctaatgaa aatagcgcat agttgctgaa aatcgccagg ctgatagttt cttatttgta
    10861 tgttattcat aatataaatt caaaaacgca tgcgaaacgc ttctgccatt ttcccaattt
    10921 tatgcatttg gttgttatct cgagccagtt tatgaggttc aggtttcaga atggcaatga
    10981 gcaaccatgc ttgctcatca aacgcaccct gacaatatac caaatgcgat tcgtcattcg
    11041 ttctgctgaa ttggcgcaac tgtggcggaa atggattatt cacatttgcc agatgaatat
    11101 gagcaactcg ctcaaatttg attaatggcc aagtaaacga gtcgtcgtag agtgcatcgc
    11161 gaccaaatat atctggcaaa acaccgtcac gcttatagga aataaaatcc gccgttaacg
    11221 catcaagctc ctctgctgta agttgcaggc gaataagttt tgttttgaat acccgcatcc
    11281 ttattcctta aagtcattga aatcatcatc cgtcatatca ttaagatatt tttcagctcg
    11341 gcgtgtaatt tcctctaatc ttgcagcact tggacggaag cgcgccttta tgggcttttt
    11401 ctcctcttgt tcagcaagca tgtccattaa cgcttcgaat gcgctggcac ttaagagata
    11461 tcctgcgggg cgattattag aaagaaccgc aaccggttga tcaataaagt atttagctgg
    11521 gtttttacgt aattcagtga tattgaccga tttttcagcg agaattcgat gcatacagtg
    11581 atcccctcat aataatgcac accagaatat acataatagt atacatcata atcccagcac
    11641 cagttgtctt tccatttgag ggtcaagcaa cgtcacatgc agccccacca gtcgcacacc
    11701 gcgcccgccg cggcgttcat cccaggtttt acgcgcggtg gcgattaagt cagctttatt
    11761 cagccgcggc cagacgtgct cctgggtggt ttgctgaaaa tcgtcgaact ttaatttcac
    11821 cccctggcga gcaatcagta aatcaggttt tacctttgcc agacggcgtt caagttccgg
    11881 atacagccgc tcgataatcg cttcacattc agaccagtga tgaatatctt ccgccatcgt
    11941 gcgttccacg ccgacggatt ttcgtaaccg ttcgctgtta acgtcgcgct cgtcaatccc
    12001 ctgactacgc tcccacaaaa tgcggccaaa tttgccaaag cgtttaagca gaatcaccag
    12061 atcacacttt tgtacatcac cgcaggtccg cagccccatc gcttccagct ttgccgctga
    12121 gactttgcca acaccgggaa tttttgccag cggtaaggtt tgcagaaatg ccgaaacttc
    12181 tgccggcgta atcacaaact ggccgttggg tttattcatg tcggaggcga ttttggcgag
    12241 aaactttact ggtgccacgc ccgcagacgc cgtcagttgc agctcgctga aaattgtctg
    12301 gcggatttcc tgggcgatga gggtcgcaga accgtggcaa tggacgctat cagtgacatc
    12361 gaggtaggct tcatccagtg acaacggttc aatacgcgag gtgtagcgcg agaagatttc
    12421 acggatatga tttgaggctt ctttgtaggc gtcaaagcgc cccggaagca aggtgagatg
    12481 tgggcataat ttgagcgcca tccctgtcgg catagcgctg cgtacgccaa atttacgcgc
    12541 gggataattg gcggtgctga tcaccccccg acgttcgcgg ctgccgccaa tagcaatagg
    12601 gatatcgcgc agggcgggat tgtcgcgcat ctccactgcg gcgaaaaagc agtccatatc
    12661 cacatgaatg attttacgca ttactcacct ctcaatactg gtaaagtata cagtgatttc
    12721 agggtttgag aaatgcgtaa agattcagca tttagcgcgt tcgctgcgaa agcacctgct
    12781 gcttatccag cttttgcacc agcggctgca ccactttatc cccatgctga ccaaagtatt
    12841 gatacagcgt atcggacgca cttttggtca gcaccataat ctcaatgcgt cggttgcccg
    12901 cgctttgcgg atttttcgcg tccagcagca tctggtccgc cattgcgctt acctgcatca
    12961 ctttatcttc cggcattccg gcctcttcca gcacccgacg agctgaaagc gcgcgatcgc
    13021 ccgaaaggtt ccagttgttg tagatattgt ttttgtaggc catcgcatcg gtatgcccgg
    13081 taataataat tttgttatcg agcgagtcga acactggtgc gagctccacc agcaaagttt
    13141 taaagaacgg cataatctgc gcgctgccgc gttcgaacat attgcgattc tggtcgtctt
    13201 taatcagcac gcgtaatccc tgcgggacaa tctccatctc cagattcgct tccatatgcg
    13261 catcacgggc aatggtgttg atactggttg ccagctcgcc aagctcggtg gcggatttct
    13321 tcttcagcag cgccgttttc tcattcacat cacgcgcttt tttctccgtc tcttccggca
    13381 ccgccacggt ggcaggtttt ggcggatgag acgggctgat tttatttaac ggcgacagcc
    13441 caccaccgtt aaaaatcgac tgcccgtgca gcgcggcgat aatgctttca cgctcggatt
    13501 tactgacgct gttgacaatc aactttatcg tcgtcaccaa aggggccgag cgtatttccg
    13561 aggtttctgc ccgctttacc ctggatgcga tgcccggcaa acagatggcg attgacgccg
    13621 atcttaacgc cggattgatc aaccaggcac aggcgcaaac ccggcgtaaa gatgttgcca
    13681 gcgaggccga tttctacggc gcgatggacg gggcatcgaa gtttgtgcgc ggggatgcca
    13741 tcgccgggat gatgattctg gcgattaacc tgatcggcgg cgtctgtatc gggatattta
    13801 aatacaacct gagcgccgac gccgccttcc agcaatatgt gctgatgacc atcggcgatg
    13861 ggctggtggc gcagatccct tccctcctgc tctccaccgc agcggcgatt atcgtcaccc
    13921 gcgtcagcga caacggcgat atcgcccatg acgtgcgcca ccagctgctg gcaagcccgt
    13981 cggtgctcta caccgccacc gggattatgt ttgtgctggc ggtggtgccg ggaatgccgc
    14041 acctgccgtt tttgctgttc agcgccctgc tgggttttac cggctggcgg atgagcaaac
    14101 agccgcaggc ggcggaggcg gaagagaaaa gcctcgaaac gctgacccgc accatcactg
    14161 aaaccagcga gcaacaggtc agttgggaaa ccattccgct tatcgagcct atcagtttaa
    14221 gcctcggcta caagctggtg gcgctggtcg ataaagcgca gggcaacccg ctcacccagc
    14281 gtattcgcgg cgtacggcag gtgatatccg acggtaacgg cgtgctgctg ccggagatcc
    14341 gcattcggga aaacttccgc ctcaagccca gccagtacgc cattttcatc aacggcatta
    14401 aggccgatga agcggatatt ccggcggata aactgatggc gctgccctcc agcgaaacct
    14461 acggcgagat tgacggcgtg ctggggaacg acccggcgta cgggatgccg gtgacctgga
    14521 tccagcccgc gcagaaggcg aaggcgctga atatggggta tcaggtgatc gacagcgcca
    14581 gcgtgattgc cacgcatgtg aacaagattg tgcgcagcta tattcctgat ttgtttaatt
    14641 atgatgacat cacgcagctg cataaccgcc tgtcgtcgat ggccccgcgc ctggcggaag
    14701 atttgagcgc ggcgctgaat tacagccagt tgctgaaagt gtaccgtgcg ctgctgaccg
    14761 aaggcgtttc cctgcgcgat atcgtcacca tcgccaccgt gctggtcgcc agtagcgcgg
    14821 tgactaaaga tcatattctg ctggcggccg atgtgcgcct ggcgctgcgg cgcagcatta
    14881 cccatccgtt cgttcgcaag caggagctga cggtgtatac gctgaataat gagctggaaa
    14941 atctgctgac taacgtagtg aatcaggcgc aacagggcgg taaagtgatg ctcgacagcg
    15001 tgccagtgga cccgaatatg ctcaaccagt tccagagcac gatgccgcag gtgaaagagc
    15061 agatgaaagc ggcggggaaa gacccggtgc tgctggtgcc gccgcagctg cgccctttgc
    15121 tggcgcgcta tgcaaggttg tttgcgccgg ggctgcatgt gctgtcgtat aacgaagtgc
    15181 cggatgagct ggagttgaag ataatggggg cgttgagttg attggccttt aaggggcgca
    15241 tccggcattg gtgcacaatt gccggatgcg gcgtgaacgc cttatccggc ctacggttga
    15301 gtgcagtttt gtaggcatga taagacgcgc cagcgtcgca tcaggcatcc ggcaccagcg
    15361 cacaattatg aaatgatacg ctccccggca ctaaaatccg ttatgtctcc cgcccaatca
    15421 atgggatata accctcgcgc cacatcgcga tggaacgttg agaatggcca atcactcact
    15481 tgctttaccc agccatgttt tactggattt atataaatat aatcaacatg atggcgatag
    15541 tctttggtat tgcggatggt gtgctcctaa aaacgtggtt gccagatatt tttcaatcca
    15601 caagcatggg tgaactgctt tttaatctcc cgccagcggg aggaaaaatc atcatcgctt
    15661 tcgggtaatg tccatatgca atgcatatgc tccggcaaaa cgacccaggc gttaatttca
    15721 aaaggtctgt ctcgcttaac gttaataatg gcgttacgga gcatctggaa ctgagcggtc
    15781 agaaggtggc ttcgacgatt tcgtaggtta accgtgaaaa accaggttcc ccctttgatg
    15841 taataccgac gatattcaga catggcaact cctttttctc aacggtaaga agaaaaggaa
    15901 aggtcggcaa tgaggtgatg gaataacggg aaagcggctc gcaaaaacgc acagatttgt
    15961 aggcatgata agacgcgtca gcgtcgtatc aggcatctgc gcacaactgc cggatgcggc
    16021 gtgaatgcct tatccgtcct acaaaatcgt ctcttccgtc aaaatccggc gcgcgcctaa
    16081 aaagtgatcc tgccagaaag gttcagctaa ccgacttacc cgaatggttt ccccggtgcg
    16141 cggtgactca ataaactgac cgtcgcccag atacaccccc atatggtcgg caatctcgcg
    16201 gctgtggata tggaaaaaca gcaaatctcc ccggcgcagg tcgttattcg ccacaatcgt
    16261 tgcccgatgg tagtgataca tctcattagc cgtacgcggg agcttcgcct cgaggatctt
    16321 gttgtaggca taaaaaacca acccgctaca gtcaaagcct ttatcaggcc gcgtgccgcc
    16381 ccagacgtaa ggcttgccaa gctgctgctc caggcggtga atcgccactt cggtaatgtt
    16441 atgcagatgg tcgctgctta aaaagtggtt gttttccgcc agcgcctgcc agcgtctgtc
    16501 actcgccttg agcggtgcag gaaaccattc tggatgctgt tttatctgtt cgcggttgtg
    16561 ctggcgtagc gcccttctgc tttcggcatt gccttccaca atatagctga cctgcttttt
    16621 cagatgggtt tggtactgtt ttaataaacg cgcacggttt tgcatccgct ggcgggcggc
    16681 gtagctgaaa gagatatgtg atgtggtggc gctggcaaat gaagaaaacg gcaaacaaat
    16741 caacagtaat cccgacaata caaaggatgg aatcgacggc aaggacatac tcaataatgg
    16801 ctggtcatgc attgatgtta cagtgtatcc cggccc
//
LOCUS       DBFIFM010000100        16746 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.100, whole genome shotgun sequence.
ACCESSION   DBFIFM010000100 DBFIFM010000000
VERSION     DBFIFM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.100"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..873
                     /locus_tag="N7C74_002753"
     CDS             <1..873
                     /locus_tag="N7C74_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HGU2432587.1"
                     /translation="VKVLLIALPEMRQLSLAKEDVHNLREGARYLTSKDVISYLLTQH
                     DVPTVWDELARDKLLPLEYKKQLWQRTLNLMMSKRQEDQEQAYEVQLALIDNGVVDEE
                     MLNNAIDLLVDLPAEYRYRMRNQLFDNKELPSGIINKLDQQYRFNSDWALAVVSMKNS
                     TRRQSERGLHRWNHEDSDIFAELATIKDKSDDEWWRALLQSRNDHLRQTALRNAHTPA
                     SLLTTLTESQDRSLAINNPQLAADVKTVWLKEDPSLLLFVDKPDLSQLRDLVKTGATR
                     KIRNEARHRLEEKQ"
     gene            870..1319
                     /locus_tag="N7C74_002754"
     CDS             870..1319
                     /locus_tag="N7C74_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432588.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNGGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKNYCPP"
     gene            complement(1404..2315)
                     /gene="rdgC"
                     /locus_tag="N7C74_002755"
     CDS             complement(1404..2315)
                     /gene="rdgC"
                     /locus_tag="N7C74_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HGU2432589.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMANPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            2440..3348
                     /gene="mak"
                     /locus_tag="N7C74_002756"
     CDS             2440..3348
                     /gene="mak"
                     /locus_tag="N7C74_002756"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HGU2432590.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(3491..4675)
                     /gene="araJ"
                     /locus_tag="N7C74_002757"
     CDS             complement(3491..4675)
                     /gene="araJ"
                     /locus_tag="N7C74_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HGU2432591.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(4801..7944)
                     /gene="sbcC"
                     /locus_tag="N7C74_002758"
     CDS             complement(4801..7944)
                     /gene="sbcC"
                     /locus_tag="N7C74_002758"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HGU2432592.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQNTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLMLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(7941..9143)
                     /gene="sbcD"
                     /locus_tag="N7C74_002759"
     CDS             complement(7941..9143)
                     /gene="sbcD"
                     /locus_tag="N7C74_002759"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HGU2432593.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            9333..10022
                     /gene="phoB"
                     /locus_tag="N7C74_002760"
     CDS             9333..10022
                     /gene="phoB"
                     /locus_tag="N7C74_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HGU2432594.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            10080..11375
                     /gene="phoR"
                     /locus_tag="N7C74_002761"
     CDS             10080..11375
                     /gene="phoR"
                     /locus_tag="N7C74_002761"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HGU2432595.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSVISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIVKNSD"
     gene            11782..13101
                     /gene="brnQ"
                     /locus_tag="N7C74_002762"
     CDS             11782..13101
                     /gene="brnQ"
                     /locus_tag="N7C74_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HGU2432596.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            13177..14550
                     /gene="proY"
                     /locus_tag="N7C74_002763"
     CDS             13177..14550
                     /gene="proY"
                     /locus_tag="N7C74_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HGU2432597.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            14706..16523
                     /gene="malZ"
                     /locus_tag="N7C74_002764"
     CDS             14706..16523
                     /gene="malZ"
                     /locus_tag="N7C74_002764"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HGU2432598.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGREVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     MALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVVQWQRKEGHGQLTDGILALPAISATVWMN"
BASE COUNT         3763 a         4203 c         4530 g         4250 t
ORIGIN      
        1 gtaaaggtgt tattgatagc gttgccggag atgcgtcaac tgtctctggc aaaagaagat
       61 gtgcacaatt tgcgcgaagg tgcgcgttat ctgacatcga aggacgtgat tagttatcta
      121 ttgactcagc atgatgttcc caccgtctgg gatgagctgg cacgcgataa attattgccg
      181 ctggaatata agaaacagtt atggcaacgc acattaaatt taatgatgag caaacggcaa
      241 gaagatcaag agcaagccta cgaagtacag ctggcgctga ttgataatgg tgttgttgac
      301 gaagagatgt taaataatgc cattgattta ctggttgatt tacccgctga ataccgctat
      361 cgaatgcgta accaattatt tgataataaa gaattacctt ccggaattat caacaaactc
      421 gatcaacagt atcgcttcaa ctctgactgg gcgctggctg ttgtcagtat gaaaaatagc
      481 acccgtagac aaagtgaaag aggattacac cgctggaacc atgaggatag cgatattttt
      541 gcggaactgg cgacgataaa agataaatca gatgatgaat ggtggcgtgc attgctgcaa
      601 agtcgcaatg atcatttgcg ccaaacggca ttacgcaacg cacatacccc ggcgtcgttg
      661 ttaacgacat tgactgagtc tcaggatcgg tcgctggcta ttaataatcc acagctggct
      721 gccgatgtga aaacggtgtg gctaaaagag gatccatcat tacttttatt tgtcgataaa
      781 cccgatcttt cgcagttacg ggatttagtg aaaaccgggg caacgcggaa aattcgcaat
      841 gaagcgcgtc accggcttga ggaaaaacaa tgacacaacg cccctggagc aagttacagc
      901 gggagatcta tgacttactg acgccaacga tcaacctgca aattcactgt acccgttatc
      961 ccatgcgcag ccaaaatggt ggaagcaccg atttaccgcg ttattggatc acgttggata
     1021 aaaacgtgat ctgggattac ccgaaagact ttattgcagg caacggtgga gtgcgtaatt
     1081 ttcacggtga aacttgctgg tatccttacc tgacggatat ttgttcaatt tctgatttat
     1141 tgcgtgaata tattgacacc ccaaaagcgg agttgttaac aaaacaattt accagcgata
     1201 aatgggggtt agtgaatatt ctgcgtgctg ctgacaggcg aatagggatg aggcgattag
     1261 atcagttacg gcgtaaaacg cataatattg ctgcgctaaa aaattattgc ccgccgtagc
     1321 gaataattat atgcctggtg tggcttcgta cgccggataa gacgcggcag gcgtcgcatc
     1381 cggcattaaa ggaaaatcag caattaacgt tgtgcttcgc cacctaatcc ttcaatcagg
     1441 ttttgaatta acgctgccag ttcaccagtc atcaggatga aatcggcatc aaaacgctgg
     1501 gcgaaatctt cacggtcgat atcttcgttt tgatcgcgca gctcgtcgca gaacttcaga
     1561 cgcttgagcg aaccatcgtc gcacatcaca aactgaatgc gctgctgcca gtcgagcgcc
     1621 agtttagtca ccacttttcc ggcttcaatg tgattggtga tctcttcgct ggtcagatct
     1681 tgtttcttcg cgcggatcac tccgccatct tccagcaacg atttcagctc ggcttcatca
     1741 agcagctgga agccctgtgc cgcactaccg gaacgtaccc attcggtcag cgtcagttca
     1801 atcgggtttg ccatgctcaa tggtacaacc ggtaacgacc ccaggctttt acgcagcagt
     1861 gccagcgtat cttccgcttt tttggcactg gcgcagtcca ccataatcaa accgttaacc
     1921 gtgtcgatcc acatcattgt ctggctaaaa cggctgaaag cacgcggcaa cagagagtgc
     1981 agcacttcgt ctttcagcga atctttttcg gttttcttca gcttacgcgc ctgttccgct
     2041 tccagtttgg cgattttcgc ttccagcgcc tgtttaatca ccggagacgg taggattttt
     2101 tcttctttgc gcgcgcagat aacaatttga ccattggcaa cgtgcgttaa cgcatcgctg
     2161 tgcgatccca tcggaggaac ccagcccatc ttcgccatgt cctggctgcc gcatggggta
     2221 aatgccatcg aggctagctg tttttccatc tcttctgcac gcagcgaaat ctcgcggcta
     2281 agacggtaaa ccattaaatt tttgaaccac agcatgataa tttccacggc cttgtcgtta
     2341 aatttagcgg gcatgataac gaattgtcgg cggccttgca ttgccaatcc ggttgtccgt
     2401 ctctacgcta ttgatattga aaaaaataag gagagtaccg tgcgtatagg tatcgattta
     2461 ggcggcacca aaactgaagt gattgcactg ggcgatgcag gggagcagtt gtaccgccat
     2521 cgtctgccca cgccgcgtga tgattaccgg cagactattg aaacgatcgc cacgttggtt
     2581 gatatggcgg agcaggcgac ggggcagcgc ggaacggtag gtatgggcat tcctggctca
     2641 atttcgcctt acaccggtgt ggtgaagaat gccaattcaa cctggctcaa cggtcagcca
     2701 ttcgataaag acttaagcgc gaggttgcag cgggaagtgc ggctggcaaa tgacgccaac
     2761 tgtctggcgg tttcagaagc agtagatggc gcggcagcgg gagcgcagac ggtatttgcc
     2821 gtgattatcg gcacgggatg cggcgcgggc gtggcattca atgggcgggc gcatatcggc
     2881 ggcaatggca cggcaggtga gtggggacac aatccgctac cgtggatgga cgaagacgaa
     2941 ctgcgttatc gcgaggaagt cccttgttat tgcggtaaac aaggttgtat tgaaaccttt
     3001 atttcgggca cgggattcgc gacggattat cgtcgtttga gcggacatgc gctgaaaggc
     3061 agtgaaatta tccgcctggt tgaagaaagc gatccggtag cggaactggc attgcgtcgc
     3121 tacgagctgc ggctggcaaa atcgctggca catgtcgtga atattctcga tccggatgtg
     3181 attgtcctgg ggggcgggat gagcaatgta gaccgtttat atcaaacggt tccgcagttg
     3241 attaaacaat ttgtcttcgg cggcgaatgt gaaacgccgg tgcgtaaggc gaagcacggt
     3301 gattccagcg gcgtacgcgg cgctgcgtgg ttatggccac aagagtaaaa aacccaggca
     3361 attggcgcat catgcctgat gcgacgcttg ccgcgtctta tcaggcctac aaaatgtgcc
     3421 agaaccgcgt agggcggata aggcgttcac gccgcatccg gcaataagca acgcacaccg
     3481 tgacagtaat ctaccccagt ggtttcgcca gcaccggact atccgccgct tgctggcgct
     3541 tatagcgacc atacagcagc aacgatgaca tcgcagcaaa cgaaagcagg gcggcaggca
     3601 gcgccacgta attatatgcc agccccagcg tcagcatcat acctccgcaa tatgcgccga
     3661 cggcgctacc gaggttaaac gctatttgcc cacctgcggc acctaataac tctccgcctt
     3721 tggcattttg cagtaacaat atttgcagag gtgctgaaag ggcaaataat cccgcgcaac
     3781 aaataaaagc aaaaataagc gacgttgttt tcatgccgcc gcagaaaaag agcatcagca
     3841 gtgccagtac aattataaag tcagtcactg ctgcaatgcg cagtggtgaa taacgtcctg
     3901 aaatcctgcc acttagcata tttcccagca ccatccctag cccaactaac atcataataa
     3961 aggtcatcgc cgtttccgaa aaaccggaaa taaacatcat gtatggcttt acgtagctga
     4021 accaggcaaa cacacctgcg ttgccaaaca tggtggcggc gaaaattaac cacggggccg
     4081 ggctgcgcaa aaagtgaaat tgttcgcgca gctttccttt cgcctcgtcg cgaatatccg
     4141 gcacccaaaa atagaccgat gccatcaccg caatattaaa aacagcgatc aataaaaagg
     4201 tgtaacgcca gctaaattcc tgacttaaat acgttcccag cggaatgccc agcaaattgg
     4261 cgactgtcat cccggaaacc atccccgcca cggcggcggt gacttttccg ggtttgataa
     4321 tttttgataa cacgatcgct ccgacgccaa aaaatgcgcc atgcggaaag ccggaaacca
     4381 ggcgaccaat ggcaagcatc aggtacgacg aagagagcgt gaacatggcg ttgccaatga
     4441 cgcacaacgc caccagaaac aacaagatat gtttgagtga gtagcggctg gaaaagagtg
     4501 cgattattgg cgcaccgacc accaccccca gcgcataata cgagatcata tgcccggcag
     4561 caggaatcga aattcctacg ttatgcgcca gctccgtgag cacgcccata atgccaaatt
     4621 cggccatccc caaaccaaac gtgcccagag ccaaagataa aatgactttt ttcataccac
     4681 catcacaact accagaacgg ctaccaatca ccgtagccaa taaagtgata gcttgcagga
     4741 taacgaccag caaggccaag agataattcg cccctctgta ttcattatcc tgctgaatag
     4801 ttatttcact gcaaacgtac tttccagttt gctgtagccc aggccgttga tcttcttcac
     4861 tttgatctgc acaggaatac gctctttcat cgcttcaacg tggctaatca caccgatggt
     4921 tttgccactg gcgttcaggg catccagcgc atcaagggcg gtatccagcg tttcgctatc
     4981 cagcgtgcca aaaccttcat caaggaacag cgagtcaata cgtgttttat ggctgaccag
     5041 atccgaaagc gccagcgcca gcgccagact aacgaggaaa ctttcgccgc cggaaagggt
     5101 acgggtatcg cgtaccgcat ctgcctgcca ggtatcaaca acctcgactt ccagcgcctc
     5161 gctggctttg cgctgtaaca gatagcgccc gtgcagccgg gtaagttgct gattagcgag
     5221 atggactaaa ttatccagcg tcagcccctg ggcaaacttg cggaatttat cgccctcttt
     5281 ggaacctatt agcgaattca gatatcccca gtcctcaacc tgctgcgtca tttgagcaat
     5341 ttgctgcagt aaggtttgtt gttgctgacg gttatctgca tcctgcttca gctgctggcg
     5401 aatctcgcct tgactcgtgg tgttttcacg caacttttgg tgagtttgcg ctaactcttg
     5461 ctgaatctgc tccaccgtca cagtgagagc caacccgtca ggtcggtgtt gttgatgctg
     5521 tgccagcgtt tctgctgtct gagtgaccag cgtttgcgcc tgacggcgct ggttttccag
     5581 attctgcttg agctgttcca gctgcgttag tgtttgttca tccattagcg ccgcaaggaa
     5641 cgcctgctga tcgtcaaaga cgctggcctg tagcgcggtg tcaaactgcg cctgggcttt
     5701 ttgcagactt tgcgccgcca gaacatcctg ttgctgtaac gtctgctgct ggctgtgtaa
     5761 tgcgagacat tgttcatgaa cctgccgcca gttatccagc gccacagttt cttcgctgtg
     5821 cgggagatca tcactttgcg gcaacgtttc cagaatcggc gtcagctgct gaatacggtt
     5881 ttgcagcgcg gttaattcgt tctggcgttg ctgccagctc tgcgcttctt gctgacgtgt
     5941 cgccaaccag ctctcttctt catcttcctg tggcaatgtc agtgcataac ccgccaatgc
     6001 cgttaagagc tgctgctggc gttgttcaat ttgctgttga tactggataa tttgctgatt
     6061 atgcgcggca atctgccctt gtaattcatg tcgttggctg agtaaccgca gctggcgttc
     6121 gtgctcatct tgtgcatcca gccacggttg aatatcgtcc tgtggctgca aggtgatatt
     6181 gaggctggcc gtgacggctt gccattgttg agtaagtgct tgctcatctt gtcggaggct
     6241 ttgcgcttcg ttttcatcgc gctgaagctg cttcgttaat gcatccagtt gcccgcgcag
     6301 cgccgcacct tcttcgccga gctttttaac ttcgttttcc agcgccagta atcgagactg
     6361 attaacgcca ggctccagcg cctgatacgc ctcgaccgcc ggatggctgg tggaaccaca
     6421 aagtggacaa ggctgacccg cctgtaactg tgcacgttga gcttccagcg ttttgatgcg
     6481 cgcttcctgc tcgcaaatgg ttttcacatc ggcaagttgc tgcgtctttt ctttataacg
     6541 ctggcgcatt tcgttaagtg cggcattacg ttgcgtctgt tccagagtga cattctggat
     6601 agtgaccatt aactgcgcca gacgtttttg ttggggaaca atctgcccat gcagcgcgac
     6661 caggcgctga cgcagtgggc gttgctcagc atgttgcgcc agggcggtag caacttcatc
     6721 ggcggttaac atcaacgtga tcgccgcaag cgcattaagt ttttgctcag catgggttaa
     6781 ctgttgctgc cattgccgca gatgctcgcg atcgctggtt tgttgggaga actgcgcacg
     6841 ccaacccgcc agttcgttgt tccactgacg gaagcggtcg tgttcctgta accaggtatt
     6901 caggctttgt tgctgctgct gtaattctgc tgactgcttc gccgcgtggt ggcgaatgct
     6961 cgcgcgaagc gccattgtgt tctgtaagcg agtatttact tcttcaatct gctggcgagt
     7021 atgcgccagc gccgcgctgt gttctgcgat gcgttcccag tgcggacgaa gatttcgtgc
     7081 cggttgtgcc agactgagcg ccgccagttg aggttgcgct ttttcttctt cggctaacgc
     7141 ctgttgcaag gcctgctgac ggcggctggc ttcttgctgc aattcgtcca gacgcgttaa
     7201 ccagtttagt gattgttgtt cttgctgctg cgcggtaagt aactgttttt cttcgtcagt
     7261 aagtacctgc aaactcgctg tcagcgattg cacttgttcc ggcgtgagca acgcgacgcc
     7321 gctggcctgc gcttgcagct tctccagctc tgtgcgggcc gatttgtgct gctcaaaaac
     7381 catcgccgag atttgcccgt agatttcagt gccggttaac tcctcgagca attccgcgcg
     7441 ttctttgggt ttggcattca ggaaggcagc aaattgcccc tgcgaaagca gcatcgaacg
     7501 ggtgaagcgc ccgtaatcca gcccggttaa cgtcgctgtc agttccagct tatctttcac
     7561 tttgtcggcg agaattttgc cgtcggcgca gcgcgccagc tctacgcgtg gcacctgcaa
     7621 attaccgtcg ggttggttac gcgcccgatt ctggctccag aacgcgcgat acgcttcacc
     7681 tttcacttca aactccacct ccgccagaca ttcagcggta tcgcgggtca tgagatcatt
     7741 ttgcgattgt gaaacgttag agagacgcgg agtttcgtga tacagcgcca gacaaatggc
     7801 gtccagcagg gtggttttcc ccgcaccggt cgggccggta atggcaaaca gcccgttgct
     7861 ggcgaacggc tcgcgggtga aatcaatctt ccattcgcct tttaatgagt tcaggttttt
     7921 caggcgcagg ctgagaattt tcatgcttcg tgttctccgg cgagggtatg caacgtcgtg
     7981 gtgaaaagat gctgcagacg ttgctgctgc gattcatcca gttcttccag tgccagacgg
     8041 cgattgaaca cctcttcgac gctgagttcg ctgagggttt cacgctgttg gctggctaac
     8101 acacgctcgc gctgttcacg actccgacgt accagcaata cttcaacagg caatgattcg
     8161 gttaatgcct ggattttgcg ctgaatatca tgcagatact catcagtagt gatttcgata
     8221 tccagccaga caggtggctc ctgcgataca tcgcgccact gttccagctg tgcggtaatc
     8281 gacgccagat cgcctttcag cactgccatg ggttgcgtta ccggtacgtt caggttttcc
     8341 acgctctcta atttgccgtt tgaaaatgtc accagatgga catatttact cttaccgcat
     8401 tcatcaaaac tcagtggaat gggggagccg caatagcgaa catgttccat gccgccaata
     8461 atctgtgcgc ggtgaatatg cccgagcgcg atgtagtcgg ctggtggaaa gttttgtgcc
     8521 ggaaacgcgt ccagcgtgcc aatataaatg tcacgcacgg cgtcactttt actggccccc
     8581 acggtcgtta aatgtcccgt ggcgatgatg ggcagaggct gatcgccgcg cagtttgcag
     8641 gcatcggcat agtgttgttg gtaataatcg gtaatcgctg ccagtaaatg ctgctgtttt
     8701 tcaataccgt taagccccgc ctggctggta ataatgtcac gcggacgtaa aaacggaatg
     8761 gggcacagca ctgcgcctgg cgtcccgtcg cgacgaggca agatttgcgg cgcatgtccg
     8821 gcgctggcga ccacggtagt attgaggaac gccatgatat cgcgcgattc attcagcgtg
     8881 gcgaccgagt catggtttcc tgccagtacc accagatgac agccagtttg ctgtaaattg
     8941 acaacaaaac ggttgtataa cgtgcgggcg taactgggcg gcgagccggt atcgaaaaca
     9001 tcaccggcaa caataatcgc atccacctga tgggtttgtg ctgtctccag cagccagtca
     9061 agaaaagcct gatgttcagc ttcgcggctt ttactgtaga agttctggcc gagatgccag
     9121 tctgaggtgt gaaggatgcg cataacggtt ccctggcgaa aaagcatggg cgcgattata
     9181 cccaaacaga tgtgccattt gcttttttct gcgccacgga aatcaataac ctgaagatat
     9241 gtgcgacgag cttttcataa atctgtcata aatctgacgc ataatgacgt cgcattaatg
     9301 atcgcaacct atttattaca acagggcaaa tcatggcgag acgtattctg gtcgtagaag
     9361 atgaagctcc aattcgcgaa atggtctgct tcgtgctcga acaaaatggc tttcagccgg
     9421 tcgaagcgga agattatgac agtgctgtga atcaactgaa tgaaccctgg ccggatttaa
     9481 ttctccttga ctggatgtta cctggcggct ccggtatcca gttcatcaaa cacctcaagc
     9541 gcgagtcgat gacccgggat attccagtgg tgatgttgac cgccagaggg gaagaagaag
     9601 atcgcgtgcg cggccttgaa accggcgcgg atgactatat caccaagccg ttttcgccga
     9661 aggagctggt ggcgcgaatc aaagcggtaa tgcgccgtat ttcgccaatg gcggtggaag
     9721 aggtgattga gatgcaggga ttaagtctcg acccgacatc tcaccgagtg atggcgggcg
     9781 aagagccgct ggagatgggg ccgacagaat ttaaactgct gcactttttt atgacgcatc
     9841 ctgagcgcgt gtacagccgc gagcagttgt taaaccacgt ctggggaact aacgtgtatg
     9901 tggaagaccg cacggtcgat gtccacattc gtcgcctgcg taaagcactg gagcccggcg
     9961 ggcatgaccg catggtgcag accgtgcgcg gtacaggata tcgtttttca acccgctttt
    10021 aacgccttgc tcatcggacg cagagcaggg cttatgattt cttaactgga gtatcttacg
    10081 tgctggaacg gctgtcgtgg aaaaggctgg tgctggagct gctactttgc tgcctcccgg
    10141 ctttcatcct gggtgcattt tttggttacc tgccctggtt tttgctggca tcggtaacag
    10201 gactgcttat ctggcatttc tggaatttat tgcgcctttc atggtggctg tgggtggatc
    10261 gcagtatgac cccgccaccg gggcgtggta gctgggaacc gctactatac ggcttacacc
    10321 agatgcagct gcgaaataaa aaacgccgcc gtgaactggg caatctgatt aaacgctttc
    10381 gtagcggcgc ggagtcgctg cccgacgcgg tggtgctgac cacggaagag ggcggtattt
    10441 tctggtgtaa cggtctggcg caacagattc ttggtttgcg ctggccggaa gataacgggc
    10501 agaacatcct taacctgctg cgttacccgg agtttacgca atatttgaaa acgcgtgatt
    10561 tttctcgccc gctcaatctg gtgctcaaca ccgggcggca tctggaaatt cgcgtcatgc
    10621 cttataccca caaacagttg ctgatggtgg cgcgtgatgt cacgcaaatg catcaactgg
    10681 aaggggcgcg gcgtaacttt tttgccaacg tgagccatga gttacgtacg ccattgaccg
    10741 tgttacaggg ttacctggag atgatggatg agcagccgct ggaaggcgcg gtacgcgaaa
    10801 aagcgttgca caccatgcgc gagcagaccc agcggatgga agggctggtg aagcagttgc
    10861 taacgctgtc gaaaattgaa gccgcaccga cgcaattgct caatgaaaag gttgatgtgc
    10921 cgatgatgct gcgcgttgtt gagcgcgagg ctcagactct gagtcagaaa aaacagacat
    10981 ttaccttcga gatagataac ggcctcaagg tgtctggcaa cgaagatcag ctacgcagtg
    11041 tgatttcgaa cctggtctat aacgccgtga atcatacgcc ggaaggcacg catatcaccg
    11101 tacgctggca gcgagtgccg cacggtgccg aatttagcgt tgaagataac ggaccgggca
    11161 ttgcaccgga gcatattccg cgcctgaccg agcgttttta tcgcgttgat aaagcgcgtt
    11221 cccggcaaac cggcggtagc ggattagggt tagcgatcgt gaaacatgcg gtgaatcatc
    11281 acgaaagtcg cctgaatatt gagagtacag taggaaaagg aacacgtttc agttttgtta
    11341 tcccggaacg tttaattgtc aaaaacagcg attaatccgc ctttgtcatc ttttattgcc
    11401 ataagccagc cgatgctggc ttattttctt tgcagtcaaa atacgggcgt tagattttac
    11461 aacggttggt gattttttgt tcgcatgatt agccatgtct ttttcacgga aatagtgttt
    11521 tatactggtt ggtgatttct tatcgctata tacctctggt ttttagatcc ctccttgctt
    11581 taaaacgtta taagcgttta aattgcgctt caggtgctgt catactgact gcattaacgc
    11641 ggtaaatcga aaaactattc ttcgccgcgc ctggttggga gtatttcccg ctaaaattgt
    11701 ttaaatatac cgctgtatca tccccaggga ttggcacaaa aatttaacgt tacaacacca
    11761 catccacagg cagtatgatt tatgacccat caattaagat cgcgcgatat catcgctctg
    11821 ggctttatga catttgcgtt gttcgtcggc gcaggtaaca ttattttccc tccaatggtc
    11881 ggcttacagg caggcgaaca cgtctggact gcggcattcg gcttcctcat tactgccgtt
    11941 ggcctgccgg tattaacggt agtggcgctg gcaaaagttg gcggcggtgt tgacagcctc
    12001 agcacgccaa tcggtaaagt cgctggcgta ctgctggcaa cggtttgtta cctggcggtg
    12061 gggccgcttt tcgctacgcc gcgtacagct accgtttctt ttgaagtggg gattgcgccg
    12121 ctgacgggtg attccgcgct gccgctgttt atctacagcc tggtctattt cgctatcgtt
    12181 attctggttt cgctctatcc gggcaagctg ctggataccg tgggcaactt ccttgcgccg
    12241 ctgaaaatta tcgcgctggt catcctgtct gttgccgcga ttgtctggcc ggcgggttct
    12301 atcagcacgg cgactgaggc ttatcaaaac gctgcgtttt ctaacggctt cgtcaacggc
    12361 tatctgacca tggatacgct gggcgcaatg gtgtttggta tcgttattgt taacgcggcg
    12421 cgttctcgtg gcgttaccga agcgcgccta ctgacccgtt ataccgtctg ggctggcctg
    12481 atggcgggtg ttggtctgac tctgctgtac ctggcgctgt tccgtctggg ttcagacagc
    12541 gcgtcgctgg tcgatcagtc tgcaaacggc gctgctattc tgcatgctta cgttcagcac
    12601 acctttggcg gcggcggtag cttcctgctg gcggcgttaa tcttcatcgc ctgcctggta
    12661 acggcagttg gcctgacctg tgcttgtgca gaattctttg cccagtacgt accgctctct
    12721 tatcgtacgc tggtgtttat cctcggcggc ttctcgatgg tggtttctaa cctcggctta
    12781 agccagctga tccagatctc cgtaccggtg ctgaccgcta tttatccgcc gtgtatcgca
    12841 ctggttgtat taagttttac acgctcatgg tggcataatt cgtcccgcgt gattgctccg
    12901 ccgatgttta tcagcctgct ttttggtatt ctcgacggga tcaaagcatc tgcattcagc
    12961 gatatcttac cgtcctgggc gcagcgttta ccgctggccg aacaaggtct ggcgtggtta
    13021 atgccaacag tggtgatggt ggttctggcc attatctggg atcgcgcggc aggtcgtcag
    13081 gtgacctcca gcgctcacta aatcactgaa catttgtttt aaccacgggg ctgcgatgcc
    13141 ccgtggtttt ttattgtgtt gatgggttag gaattgatgg aaagtaagaa caagctaaag
    13201 cgtgggctaa gtacccgcca catacgcttt atggcactgg gttcagcaat tggcaccggg
    13261 ctgttttacg gttcggcaga cgccatcaaa atggccggtc cgagcgtgtt gttggcctat
    13321 attatcggtg gtatcgcggc gtatatcatt atgcgtgcgc tgggggaaat gtcggtacat
    13381 aacccggccg ccagctcttt ctcgcgttat gcgcaggaaa acctcggccc gctggcaggt
    13441 tacattaccg gctggaccta ctgctttgaa atccttattg tcgccatcgc cgatgtgacc
    13501 gcttttggta tctatatggg tgtctggttc ccgacggtgc cgcactggat ttgggtactg
    13561 agcgtggtgc tgatcatttg cgccgtaaac ctgatgagcg tgaaggtatt cggtgagctg
    13621 gaattctggt tctcgttctt taaagtcgcc accatcatca tcatgattgt cgccggtttc
    13681 ggcatcatca tctgggggat tggcaacggc gggcaaccga ccggtattca taacctgtgg
    13741 agcaacggcg gcttcttcag taacggctgg cttggcatgg taatgtcgtt gcaaatggtg
    13801 atgtttgctt acggtgggat cgaaattatc gggattaccg ccggtgaagc gaaagatcct
    13861 gagaaatcga taccgcgtgc gattaactcc gtgccgatgc gtattctggt gttctacgtc
    13921 ggtacgctgt tcgtcattat gtctatctac ccgtggaatc aggttggcac tgccggtagc
    13981 ccgttcgtgc tgacgttcca gcatatgggc attacctttg ccgccagcat tcttaacttt
    14041 gttgtgctga ctgcttcgct gtcggcaatt aacagtgatg tatttggcgt aggccgtatg
    14101 ctccacggta tggcagagca gggcagcgcg ccgaaaattt tcagcaaaac gtcgcgtcgc
    14161 ggtattccgt gggttacggt gctggtgatg actaccgcgc tgctgtttgc ggtgtatctg
    14221 aactacatca tgccggaaaa cgtcttcctg gtgattgctt cgctggcaac cttcgccacg
    14281 gtgtgggtgt ggattatgat cctgctgtcg caaattgcct tccgtcgccg tttgccgcca
    14341 gaagaagtta aggcgctgaa atttaaagtg ccgggtgggg tagcaacgac catcggcggg
    14401 ctgattttcc tgctctttat tatcgggttg attggttatc acccggatac gcgtatctcg
    14461 ctgtatgtcg gtttcgcgtg gattgttgtg ctgttgattg gctggatgtt taagcgtcgc
    14521 cacgatcgtc agctggctga aaaccaataa tacttgcgtt ctgtaagccg gttcaggagg
    14581 aaatcctgat ccggctattc cgaaagttat ccgcccccgt cctcctcccc caaatatcct
    14641 tcagatgatg agtgatcctg cattaggcta tggcaaggtg atcagatttt catcacaggg
    14701 gaattatgat gttaaatgca tggcacctgc cggtgccccc atttgttaaa caaagcaaag
    14761 atcaactgct cattacactg tggctgacgg gcgaagaccc accgcagcgc attatgctgc
    14821 gtacagaaca cgataacgaa gaaatgtcag taccaatgca taagcagcgc agtcagccgc
    14881 agccaggcgt caccgcatgg cgtgcggcga ttgatctctc cagcggacaa ccccggcggc
    14941 gttacagttt caaactgctg tggcacgatc gccagcgttg gtttacaccg cagggcttca
    15001 gccgaatgcc gccggcacga ctggagcagt ttgccgtcga tgtaccggat atcggcccac
    15061 aatgggctgc ggatcagatt ttttatcaga tcttccctga tcgttttgcg cgtagtcttc
    15121 ctcgtgaagc tgaacaggat catgtctatt accatcatgc agccggacaa gagatcatct
    15181 tgcgtgactg ggatgaaccg gtcacggcgc aggcgggcgg atcaacgttc tatggcggcg
    15241 atctggacgg gataagcgaa aaactgccgt atctgaaaaa gcttggcgtg acggcgctgt
    15301 atctcaatcc ggtgtttaaa gctcccagcg tacataaata cgataccgag gattatcgcc
    15361 atgtcgatcc gcagtttggc ggtgatgggg cgttgctgcg cttacgccac aatacgcagc
    15421 agctgggaat gcggctggtg ctggatggcg tgtttaacca cagtggcgat tcccatgcct
    15481 ggtttgacag gcacaatcgt ggcacggggg gagcttgtca caaccccgaa tcgccctggc
    15541 gcgactggta ctcgtttagt gatgatggca cggcgctcga ctggcttggc tatgccagct
    15601 tgccgaagct ggattatcag tcggaaagtc tggtgaatga aatttatcgc ggggaagaca
    15661 gtattgtccg ccactggctg aaagcgccgt ggaatatgga cggctggcgg ctggatgtgg
    15721 tgcatatgct gggggaagcg ggtggggcgc gcaataattt gcagcacgtt gctgggatca
    15781 ccgaagcggc gaaagaaacc cagccggaag cgtatatagt tggagagcat tttggcgatg
    15841 cacggcaatg gttacaggcc gatgtggaag atgccgccat gaactatcgc ggcttcacat
    15901 tcccgttgtg gggatttctt gccaataccg acatctctta cgatccgcag caaattgacg
    15961 ctcaaacctg tatggcctgg atggataatt accgcgcagg gctttctcat caacagcaat
    16021 tacgtatgtt taatcagctc gacagccacg atactgcgcg atttaaaacg ctgctcggtc
    16081 gggaggttgc gcgcctgccc ctggcagtgg tctggctgtt cacctggcct ggtgtaccgt
    16141 gcatttatta cggtgatgaa gtgggactgg atggcaaaaa tgatccgttt tgccgtaagc
    16201 cgttcccctg gcaggtggaa aaacaggata tggcgttatt cgcgttgtac cagcgaatga
    16261 ttgcgctgcg taagaaaagt caggcgctgc gtcgtggcgg ctgtcaggtg ctgtatgcgg
    16321 aagataacgt ggtggtattt gtccgcgtgc tgaatcagca gcgtgtactg gtggcaatta
    16381 accgtggcga agcctgtgaa gtggtgctac ccgcgtcacc gtttcttaat gtcgtgcaat
    16441 ggcaacgcaa agaaggccat gggcaactga cagacgggat tctggctttg cctgccattt
    16501 cggctacggt gtggatgaac taaagcatct gttttatttg ttacgtcgtt agtcaccagc
    16561 cctgacacta cgcagcgcaa aaaagctgct caatgtcagg gctgaatttg ttaaatattt
    16621 gtcccgatcg atatgagcga tttttggtat tgtttgccca tgatgttatg tacaacatgc
    16681 tccaggataa ccctgtagtg gagcaaagat atgacaattt caaattcgaa tttattgaaa
    16741 ccgttt
//
LOCUS       DBFIFM010000101        16563 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.101, whole genome shotgun sequence.
ACCESSION   DBFIFM010000101 DBFIFM010000000
VERSION     DBFIFM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.101"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            331..483
                     /gene="mokC"
                     /locus_tag="N7C74_002765"
     CDS             331..483
                     /gene="mokC"
                     /locus_tag="N7C74_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HGU2432599.1"
                     /translation="MKQHKVMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(587..1717)
                     /gene="dnaJ"
                     /locus_tag="N7C74_002766"
     CDS             complement(587..1717)
                     /gene="dnaJ"
                     /locus_tag="N7C74_002766"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HGU2432600.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     TKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1806..3722)
                     /gene="dnaK"
                     /locus_tag="N7C74_002767"
     CDS             complement(1806..3722)
                     /gene="dnaK"
                     /locus_tag="N7C74_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HGU2432601.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4099..4503
                     /gene="yaaI"
                     /locus_tag="N7C74_002768"
     CDS             4099..4503
                     /gene="yaaI"
                     /locus_tag="N7C74_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HGU2432602.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNALAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4529..5242
                     /gene="msyB"
                     /locus_tag="N7C74_002769"
     CDS             4529..5242
                     /gene="msyB"
                     /locus_tag="N7C74_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HGU2432603.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSSYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5391..5957
                     /gene="satP"
                     /locus_tag="N7C74_002770"
     CDS             5391..5957
                     /gene="satP"
                     /locus_tag="N7C74_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HGU2432604.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5992..6579)
                     /gene="mog"
                     /locus_tag="N7C74_002771"
     CDS             complement(5992..6579)
                     /gene="mog"
                     /locus_tag="N7C74_002771"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HGU2432605.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6694..7647)
                     /gene="tal"
                     /locus_tag="N7C74_002772"
     CDS             complement(6694..7647)
                     /gene="tal"
                     /locus_tag="N7C74_002772"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HGU2432606.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7926..9356
                     /gene="yaaJ"
                     /locus_tag="N7C74_002773"
     CDS             7926..9356
                     /gene="yaaJ"
                     /locus_tag="N7C74_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HGU2432607.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAIIRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVYTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSRDAWDDVSQE"
     gene            9426..10202
                     /gene="yaaA"
                     /locus_tag="N7C74_002774"
     CDS             9426..10202
                     /gene="yaaA"
                     /locus_tag="N7C74_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HGU2432608.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENTRGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10355..10651)
                     /gene="yaaX"
                     /locus_tag="N7C74_002775"
     CDS             complement(10355..10651)
                     /gene="yaaX"
                     /locus_tag="N7C74_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HGU2432609.1"
                     /translation="MKKMQSIVLALSLVLVAPMATQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(10865..12151)
                     /gene="thrC"
                     /locus_tag="N7C74_002776"
     CDS             complement(10865..12151)
                     /gene="thrC"
                     /locus_tag="N7C74_002776"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HGU2432610.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESAEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12152..13084)
                     /gene="thrB"
                     /locus_tag="N7C74_002777"
     CDS             complement(12152..13084)
                     /gene="thrB"
                     /locus_tag="N7C74_002777"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HGU2432611.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVESAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13086..15548)
                     /gene="thrA"
                     /locus_tag="N7C74_002778"
     CDS             complement(13086..15548)
                     /gene="thrA"
                     /locus_tag="N7C74_002778"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HGU2432612.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15574..15687)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15629..15712)
                     /locus_tag="N7C74_002779"
     CDS             complement(15629..15712)
                     /locus_tag="N7C74_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HGU2432613.1"
                     /translation="MKRISTTITTTITTTITITITTGNGAG"
     gene            complement(15926..>16563)
                     /gene="yjtD"
                     /locus_tag="N7C74_002780"
     CDS             complement(15926..>16563)
                     /gene="yjtD"
                     /locus_tag="N7C74_002780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HGU2432614.1"
                     /translation="AAARAMKTMGFSELRIVDSQAHLEPATRWVAHGSGDIIDNIKVF
                     PTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKSSWMSHAALVFGREDSGL
                     TNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLAILIQQPTKSDTTADQHQLQAL
                     RERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRLLHDIEKNITK"
BASE COUNT         3854 a         4389 c         4112 g         4208 t
ORIGIN      
        1 cgatgtttgt taggccaata tttcaaaaat aatccagctt gtcgtcaata attgcgatga
       61 agagataaat taaagttaaa taattaaatc attaaaaaat tacgccataa aaatgcgagg
      121 cgtcttccgg attagaatcg ataaaacaga atgggttaaa gagagcgata taatagcggc
      181 gggtgcttga ggctgtctgt ctcaggcatt agctgaacgg cagatagaga aaagccccga
      241 gtgatatttt accatcaacc cgaggccccc tatatgctga acacatgtag agtgcctctt
      301 actgaccgta aggtcaagga gaagagagca atgaagcagc ataaggtgat gattgtcgcc
      361 ctgatcgtca tctgtatcac cgccgtagtg gcggcgctgg taacgagaaa agacctctgt
      421 gaggttcaca tccgaactgg ccagacggag gttgctgttt tcacggctta cgaatccgag
      481 taagagcaac ggcggggagt gatccccgcc actttacagg tgctcgcata tcttcaacgc
      541 accctatttt tacccaggcc tgcccacggg caggcttttg gggaggttag cgggtcaggt
      601 cgtcaaaaaa cttcttcaca ccatcaaaga agctctttga gcgcgggctg ttgtgctcgc
      661 cggttgggcc accgaagctt tcttgcagct cttgcagcag ctgtttctgc ttctcgttca
      721 ggcctaccgg tgtttcgacg acaacgcggc acagcaaatc accctgtgcg ccaccgcgga
      781 cagacttgac gcctttaccg cgcatacgga atagcttacc ggtctgggtt tcgccaggca
      841 ctttcagttt gacgcgacca tcaagggtcg gtacttcgat ttcaccaccc agcgccgcca
      901 tagcgaagtt gatcgggact tcgcaataca ggttgttgcc ttcacgctcg aaaatcgggt
      961 gctgtttaac ctgaacctga acgtacagat cgcctgccgg tgcgccgtgt tcacccgctt
     1021 caccttcgcc cgcaagacgg atgcggtctc cagtgtccac ccctgccggg attttaacgg
     1081 acagcgtttt gctgcgctca acacgaccat gaccatgaca tttgttgcac ggatctttga
     1141 tcagcgtacc gcggccctga cagtgtggac aggtctgctg cacggcaaag aaaccctggc
     1201 gcatctgcac ctgaccagaa ccatgacagg tcggacaggt ctgcggctgt gtacctggtt
     1261 ttgcaccgct accgtggcaa acgtcacact cttccagagt cggaatgcgg atctctttgg
     1321 tcacgccacg tacagcttct tcgagggtga gctccatgtt atagcgtaaa tcagcaccgc
     1381 gcgccgcacg ttgacgacca cgtccgccgc caaaaatatc gccgaaaacg tcaccaaaaa
     1441 tatcgctgaa gtctgcgccg ccgccaaaac cgccgccgcc catgccacct tgctcaaacg
     1501 cagcatgacc atactgatcg tatgccgcac gtttttgcga gtcggtcaga acttcataag
     1561 cttccttgat ctctttaaat ttcgtctcgg cctctttgtc accctggtta cggtccgggt
     1621 ggtatttcat ggccaggcgt ttgtaggcct ttttgatttc acgctcttcc gctgttttgg
     1681 aaacgcctaa aatctcgtaa taatcttgct tagccatctt ttttaaattg cccctagatg
     1741 aatgcacggg cggagaggaa attccccttc gcccgtgtca gtataataac ccgtttatag
     1801 ggcgattatt ttttgtcttt gacttcttca aattcagcgt cgacaacatc gtcatctttc
     1861 gcgttgtttg cagaagcatc agcaccggca gtctgctgct gggcatgttg ctgctgggcg
     1921 atttccatca gtttctggga aacctgtgcc agttcctgca ttttcgcttc gatagcggct
     1981 ttgtcttcac ctttcagagc agtttccagt gcagtcagcg cagactcgat agcagttttg
     2041 tcgtcagccg gcagtttgtc gcctgcttct tcaacctgct tacgggtgct gtgcagcaga
     2101 tggtcgccct ggttgcgagt ctgtaccagc tcttcaaact tacggtcagc ttcggcgtta
     2161 gcttcggcgt cgcgtaccat tttctggatt tcatcttcgt tcagaccaga agacgccttg
     2221 atggtgatct tctgctcttt accgctgttt ttatctttcg cggaaacgtg caggataccg
     2281 tcagcatcga tatcgaaggt aacttcgatc tgcggcatgc cgcgcggtgc cgggttgata
     2341 ccatccaggt tgaactgacc cagagatttg ttgtcagccg cacgtttacg ttcaccctgc
     2401 agcacatgga tggttaccgc agactggttg tcttcagcgg tagagaacac ctggctgtgc
     2461 ttggtcggga tagtggtgtt tttcgcgatc agcgtcgtca tcacaccgcc catggtttcg
     2521 atacccagag acagcggggt aacatccagc agcagtacgt cttttacgtc accagtcaga
     2581 acaccaccct gaacagcagc accgattgct acagcttcgt ccgggttaac gtctttacgc
     2641 ggctctttac caaagaactc agcaactttc ttctgaacca ttggcatacg agtctgacca
     2701 ccaacgagga taacgtcgtc gatatcagat acggacaggc cagcgtcctg cagcgcaact
     2761 ttcagcggct cgatggaacg gttaaccaga tcttcaacca ggctttccag tttcgcacga
     2821 gtcactttga tgttcatgtg tttcggaccg gtcgcgtctg cagtgatgta cggcaggtta
     2881 acgtcggtct gctgagcgga agacagttcg attttcgctt tttctgccgc ttctttcagg
     2941 cgctgcattg ccagcggatc gttgcgcagg tcaatgccct gatctttctt gaattcttca
     3001 accagatagt tgatcagacg gctgtcgaag tcttcaccac ccaggtgggt atcaccgttg
     3061 gttgccagaa cttcgaaggt tttttcgccg tcaacttcgt cgatttcgat aatagaaata
     3121 tcgaaagtac caccacccaa gtcataaacc gcgatagtac ggttgccagt acctttgtcc
     3181 agaccgtaag ccagcgcagc tgcggtcggt tcgttgatga tacgttttac ttccagacca
     3241 gcgatacggc ctgcgtcttt ggttgcctga cgctgagcat cgttaaagta tgccggtacg
     3301 gtgataacag cttcagttac cggttcaccc aggtaatctt cagcggtttt cttcattttt
     3361 ttcagcactt cagcagaaat ctgcggcggt gccattttct ggcctttaac ttcgacccat
     3421 gcgtcgccgt tatcagcagc aataattttg aacggcatga tggaaacatc acgctgtact
     3481 tcttcgtcct ggaagcggcg gccaatcagg cgtttaatcg caaacagggt gttttgcggg
     3541 ttcgtcactg cctgacgttt agccggctga ccaaccagag tttcaccatc ctgggtatag
     3601 gcaatgatag aaggcgtggt gcgatcgcct tcggcgttct ccagcacacg aggagtggtg
     3661 ccatccataa tcgctacaca agagttggta gtacccaggt cgataccaat tattttaccc
     3721 atctaaacgt ctccactata tattcggtca tcatgtggtt gtgagtctgt aataggggcg
     3781 aaacgtctgg tttcaactgc ccaatttcat ttttttgcag actcactcac tgcggttgac
     3841 tactaaatgg ggtcgtaacc cacgtcatca agggggagat gcaaaaattt tttgtgcttt
     3901 tgcgcagaaa tttcgcgata tggcaggaca agacgtaaaa ttgacagcaa ttttattcat
     3961 ataaagaatg aatcgcagtc aaggtattca tcaaaaaatt gtaataaaaa gaaaagatta
     4021 cgtgcctgaa tcttctcttt atcagcagta aactagtggg tattcatccc cctacctctt
     4081 cccactaaga gaatccttat gaaatccgtt tttacgattt ccgccagcct ggcgattagc
     4141 ctgatgctgt gctgcacggc gcaggcaaac gaccataaac tcctcggcgt cattgcaatg
     4201 ccgcgtaacg aaaccaacgc tctggcgctg aaacttcctg tttgtcgcat tgtgaaacgc
     4261 atacaactct ctgccgacca tggcgattta cagttaagcg gtgcatcggt ttatttcaaa
     4321 gccgcccgta gcgccagtca gagcctgaat attccttcag aaataaaaga agggcaaacc
     4381 actgactgga tcaacattaa cagcgataac gacaataaac gctgcgtctc aaaaatcacc
     4441 ttttccggtc atacggtgaa ctcatcggat atggccacgc tgaaaattat cggcgacgac
     4501 taacgccaga atatagaagc cacaaaaaat gaatgttaat tacctgaatg attcagatct
     4561 ggattttctc cagcattgta gtgaggaaca gttagcaaat ttcgcccggc tgctcaccca
     4621 taatgaaaaa ggcaaaactc gcctctccag cgtactgatg cgcaacgaac tgtttaaatc
     4681 gatggaagga catcccgagc aacatcgccg caactggcag ctgattgctg gagaattaca
     4741 gcattttggt ggcgatagta tcgccaacaa actgcgcgga cacggtaaat tgtatcgggc
     4801 cattttgctc gatgtttcaa aacgattgaa gctgaaagcc gacaaagaga tgtctacgtt
     4861 tgaaattgag caacagttac tggaacaatt tctgcgtaat acctggaaga aaatggacga
     4921 ggaacataag caggagtttc tgcacgcggt cgatgccagg gtgaatgagc tggaagagct
     4981 gctgccgctg ctgatgaaag acaaattatt ggcaaaaggc gtgtcgcatc tgctttccag
     5041 ccaactgacc cgcattttac gcacccacgc agcaatgagc gtacttgggc atggtttgct
     5101 gcgcggcgcg gggctgggag gccctgtagg cgcggcacta aatggggtta aagcggtcag
     5161 cggcagctcc tatcgcgtga cgattccagc cgtactgcaa atcgcctgcc tgcgccggat
     5221 ggttagcgcc actcaggtct gataccggaa accggggaaa attttcattt tcccctcaaa
     5281 agatcgtaga cactgcccca ctggctgatt attatgccgc gccctgaaaa cactacagtt
     5341 attcagggaa attatttcac cattcattcg atgatgattt ttgaggaatt atgggcaaca
     5401 ctaagttggc taatccggca ccgctgggcc tgatgggctt cggcatgacc accattctgc
     5461 ttaacctgca caacgtaggt tatttcgctc tggacggtat tattcttgcc atgggcattt
     5521 tctacggcgg catcgcgcaa atttttgctg gtctgctgga gtacaaaaaa ggcaacactt
     5581 tcggtttaac cgcattcacc tcttacggtt ctttctggct gacgctggtt gcgattctgc
     5641 tgatgccgaa actgggtctg accgatgcgc caaatgcaca gttccttggt gtctacctgg
     5701 gtctgtgggg cgtatttacg ctgtttatgt tcttcggcac gctgaaaggc gcacgcgttc
     5761 tgcaattcgt tttctttagc ctgaccgtgc tgtttgccct gctggcgatc ggtaacattg
     5821 ccggtaacgc cgcaatcatc cactttgccg gctggattgg tctgatctgc ggtgccagcg
     5881 caatctatct ggcgatgggt gaagtactga acgagcagtt tggtcgcacc gttctgccga
     5941 ttggtgaatc ccactaattg tcttgagatc cccccgctcg gggggatttt tttattcgct
     6001 aacgtcgcgt cttgcactct tcggtctgaa tgcagcaacc acttctggtg ccgtttcaac
     6061 gtatggccct tccagcaact gaatgcagta cggtacgctg gcaaaaatac cgtgtaccac
     6121 aacgttaccc tcagcgtcct tcacaccttc cagcgtctct ttaatagact tcggctgacc
     6181 gggtaagtta aggatcagcg cctgtttgcg aatcacaccc acctgacgcg aaaggatcgc
     6241 agttggtaca aaatgcaggc tgatctggcg catctgttca ccaaagccag gcatctcgcg
     6301 gtccgctact gccagcgtcg catcgggcgt tacgtcacga cgcgccgggc cagttccgcc
     6361 cgtggtgagc accagatggc aactcatttc atccaccagc tcacacaacg tttgctcgat
     6421 gatcgcctgc tcatcgggga ttaagcgggt ttccagttca aacggcgtgg ttagcgccga
     6481 tgtcagccat tcttccagcg cagggatacc tttatcctga taaacgccgc tggatgcgcg
     6541 atcagagatg gaaactaagc caatacgtaa agtattcatc aagatttttc cgtttaaaca
     6601 gtctcgttaa acagaatgat acactgcgaa gggagtgaca gacaggcttc agaagaggta
     6661 gcgtgaccga cttcccggtc acgctaagaa tgattacagc agatcgccga tcattttttc
     6721 cagtttttcc tggtcaatag caaacttacg gataccttcc gccagtttat ctactgccat
     6781 tggatcctgg ttgtgctgcc acaggaactc ggactcagtg atacgcgccg gacgcgcttt
     6841 cacttcgccg gtgtaagaca gtttacgttc gatagcccct tcgctctcag ccagctcttt
     6901 cagcagtgcc ggtgcgatgg tcagacggtc gcagcctgcc agttccagaa tttcgccgat
     6961 gttacggaag cttgcgccca taaccacggt ttcataaccg tgctctttgt agtactggta
     7021 gatttcagat acagaaacca cgcccggatc ttctgccgga gcgtactctt tcttatcggt
     7081 attcgctttg taccagtcaa gaatacggcc aacaaacggc gagatcagga acacgcccgc
     7141 ttccgcacaa gcacgagcct gagcgaagga gaacagcagg gtcaggttac agttgatacc
     7201 ttctttttcc agctgttccg cagcacggat accctgccag gtagaagcca gtttgatcag
     7261 aatacgatcg ttgctaatac ctgcatcgtt gtagagtttg atcaggcgtt ttgctttcgc
     7321 aattgacgct tctgtgtcat aggaaagacg cgcatcaact tcagttgaga tacggcccgg
     7381 aaccagtttc aggatttcca gaccaatatt tactgccagt ttgtcagtcg cgtccacgat
     7441 ctgctgcgcg cgatcgttgc tctgctgttt cgcccaggcg acagcatcat caatcaactt
     7501 acggtattcc ggaatctgcg ctgcgttaag aatgagagaa gggttggttg tggcatcctg
     7561 cggttgatac agcttcattg ccgcgatgtc cccagtgtcg gccactacgg tggtgtactg
     7621 acgaagggag gtcaatttgt ccgtcatgat agtatttctc tttaaacagc ttgttagggg
     7681 gatgtaaccg gtctgccctg atgatatcac gacgcaacgt cagcgcaacc gccgacaaga
     7741 cggttatcgc caggcgagac tgtttcggat ttctgacact gcgttgatat cgcccgccat
     7801 ttttaaacaa aacctcatgt atgctacgca gaagttatca agtacctcgt agcgtatata
     7861 cttcttaaac aattggtaac gtttacacag gaaagtcatc gcgaccggca ataagaggga
     7921 tatgcatgcc agattttttc tcctttatta acagcgtcct ttggggatcg gtaatgattt
     7981 acctgctctt cggcgcaggt tgttggttca cttttcgcac cggatttgtg cagtttcgct
     8041 acatccgcca gtttggcaaa agtcttaaaa atagcattca tccacagcca ggcggtttaa
     8101 cctcatttca gtcattgtgt accagtcttg cggcgcgcgt gggtagcggc aacctggccg
     8161 gcgttgcgct ggctattacc gccggtggac ctggagccgt cttttggatg tgggttgccg
     8221 cgtttatcgg catggcgacc tcgtttgccg aatgttccct tgcacaactt tataaagaac
     8281 gtgacgtcaa tgggcagttt cgtggcggac cggcatggta tatggcgcgc gggctgggga
     8341 tgcgctggat gggcgttctg ttcgccgtct ttttgctcat cgcctacggc ataattttca
     8401 gcggagttca ggcaaacgct gttgcccgag ctctgagttt ttcttttgat tttcccccgc
     8461 tggtgacagg cattattctt gctgtctttg ctctgctggc aatcattcgc ggtcttcatg
     8521 gcgtcgcccg gctcatgcag gggtttgtcc cgttgatggc gataatctgg gtactgacca
     8581 gcctggtgat ttgcgtaata aatatcgggc aacttcccca cgtcatttgg tctatttttg
     8641 agagtgcttt tggctggcag gaagcggcag gcggcgcggc gggatatacc ttaagccagg
     8701 cgattactaa cggttttcag cgcagtatgt tttccaatga ggcgggaatg gggtcgacgc
     8761 caaacgcggc agcggcagcg gcgtcctggc ctccgcatcc ggcagcgcaa ggaattgtcc
     8821 agatgattgg catttttatc gacaccctgg tcatctgtac ggcaagcgcc atgctgatat
     8881 tactggcggg taacggcaca acctacatgc cgctggaagg tattcagctt atccagaagg
     8941 cgatgcgggt gctaatgggt tcctggggtg ctgaatttgt taccctcgtg gttattctgt
     9001 ttgccttcag ctccatcgtt gccaactaca tttatgccga aaacaatctc ttctttttac
     9061 gcctgaacaa ccctaaagcg atctggtgtt tgcggatctg caccttcgca acggtcatcg
     9121 gcggcacctt gctaagtctt ccgctgatgt ggcaactggc agatatcata atggcctgca
     9181 tggctattac caatttgact gccatattac tgctctcgcc tgtggtttat accattgcca
     9241 gtgattatct acgccagcgt aaactcggcg tgcgcccggt gtttgatccg ttgcgttatc
     9301 cggatatcgg tcgccagctt tctcgggacg cgtgggatga tgtttcgcag gagtaatcac
     9361 aactatcgat caactcattc tctttttttg ctaaagtcgg cataaatttc ctgcaaggac
     9421 tggatatgct gattcttatt tcacctgcga aaacgcttga ttaccaaagc ccgttgacca
     9481 ccacgcgata tacactgccg gagctgttag acaattccca gcagttgatc catgaagcgc
     9541 ggaaactgac gcctccgcag attagcacgc tgatgcgtat cagcgacaaa ctggcaggta
     9601 tcaacgccgc tcgctttcat gactggcagc cagatttcac gccggagaat gcccgccagg
     9661 cgattctggc gtttaaaggt gatgtctaca ccggcttgca ggccgaaacc ttcagcgaag
     9721 acgatttcga ttttgcccaa cagcatttgc gaatgctttc cggcttgtat ggcgtactcc
     9781 gcccgctcga tttaatgcag ccttatcgtc tggaaatggg gatccgtctt gagaataccc
     9841 gagggaaaga tctgtatcaa ttctggggag atatcatcac caacaagctg aacgaggcgc
     9901 tcgcagcaca aggcgataat gtggtgatta atctggcgtc agatgagtat tttaaatcgg
     9961 tgaagccgaa gaaattgaat gccgagatta tcaagccggt gttcctcgat gagaagaacg
    10021 gcaaatttaa gataatcagc ttctacgcta agaaagcacg cggtctgatg agtcgtttca
    10081 ttattgaaaa tcggctgacc aaaccagagc aactgactgg ttttaatagc gaaggttact
    10141 tctttgatga agcttcctcc agcaatggcg aactggtgtt taaacgctac gagcagcgtt
    10201 aagtttgtag tcaatgccgg atgcggcgtg aacgccttat ccggcctaca aaagcatgca
    10261 gattcaatat attgcagaat tcacgtaggc ctgataagac gcgccagcgt cgcatcaggc
    10321 gctgaatgcc ggattgttac ccggcatttg tcatttagcg gtgatgtttg cctggaccat
    10381 gaccgccgtg atgatcatga tgagctttct tatggtgacg cggcggtggc ggcggtccgt
    10441 gtgggtgcca gcgattgcct cgccattcat aatgttgttt ccaccagccg tggtcgcgcc
    10501 agtgaccgcc atcccagtaa taaccgcgat tatcacgatc gcctatctgt aattttactg
    10561 acgggactaa cgtaatttcc gcagcctgcg ttgccatggg agcgaccaga accagggaaa
    10621 gtgcgagtac gatagattgc atctttttca caggttactc cttttatcat tgcccgttat
    10681 gggtcgatat ccggagatta cgcgatgaga gcggcggttg ttattctccg caatcctaat
    10741 ctctaagtta ccgcatttat tgagaatttc tcctttttcc ctgtcgtttc tctccatatt
    10801 ttcttcacaa aaaagccggg ctgcataaaa gcaaacccgg cctgattgag ataatgaata
    10861 gatgttactg atgattcatc atcaatttac gcaacgcagc aaaatcggcg ggcagattat
    10921 gcgaaagcaa gggtaaatcg gcacgttctg ccagctcttt tggcagatcc aacgtttcac
    10981 cgagaatcgc ttccgcgctc tctttaaatt tcgccggatg cgcggtgccg aggaacaagc
    11041 catattcgcc tggattcaac tggtcacgca gcgcacgata cgctacggca gcgtgcggct
    11101 ccgaagtgta gcccagttct tttaactcac gcattgtctg ttgcgtggtt tcatcatcca
    11161 cggcggcata acccagctct ttcagttgcc agattttgcg gcggaacaac tcttccacac
    11221 gcggccagtt gttcggctga ctcacgtcca tcgcgttgga taacgtcgcc tgagtcgctt
    11281 tgggtgacca ctgaccgtca tgcaggaaac gtggcacggt atcgttcacg ttggtcgcag
    11341 caataaaacg tttcaccggc agaccgagtg acttcgccag cagacccgcc gtcaaatcgc
    11401 cgaagtttcc gcttggcacc gagacaacca gctggttgcg cgcttcctgc ggcagctgcg
    11461 caactgcttc aaagtagtag caaatctgcg ccagcaaccg gctaatgtta atcgagttag
    11521 ctgagtttaa ccccagcgcc actttcagct cttcatcatc aaacgcctgc ttcaccagcg
    11581 cctgacaggc atcgaaatcg ccgtcgatgg caacagtttc gatattgccg cccaatgtac
    11641 agaacagttt ttcttgcagt ggactgattt tgcctcgtgg ataaaggata accactttca
    11701 cattcggtaa accgtagaaa gcatgagcca ctgccgctcc ggtatcaccg gaggtcgcgg
    11761 tcagaatggt cactggctta tcgcccgcaa tatgggtcag catttgtgcc ataaagcgac
    11821 cgccgaaatc tttaaatgcc agcgttggcc cgtggaacaa ttccagacaa ccgacatcgc
    11881 tttcaacatt ggcgaccgga gccgggaagg caaacgccgc gcgcacgcgc tcttccagga
    11941 tttcctgcgg gatttcatca ccaataaacg ccgagaggat cttcgcactg cgggtgacaa
    12001 aatccagctt cagcatctca tcaatttcag tcaggctgaa ttccggcagg tcgtgcggaa
    12061 aaaacagccc ctgatttttg cccaacccct gggttacggc ttgcgcaaag ctgacctgct
    12121 cattgtgatc tttaagattg tagagtttca tttagttttc cagtactcgt gcgcccgccg
    12181 tatccagccg gcaaatatga acaaaacctt cctgattttg caggtagttc ttacccaacc
    12241 agtcggcaac gcgctgggcg gtatccggct tgtcacacag agcaaacaag gtcgggccgg
    12301 agccggagat accgctcgct accgcgccga tttccgcgac cgcctgccgc gcctgccgga
    12361 agccaggcag taaccgttca cggtagggtt ctgcgataac atctttcatc agcttcgcgg
    12421 caagctcagg ctgacgggaa tagcaggcgt gaatgaagcc tgccaagtgt cgcccgtgcg
    12481 caatgcaatc ctggcggcga tactgcgccg gtaaaatagc ccgggcttct gccgtcgaga
    12541 ctttaatccc cggatacgcc agcacccaca gccactcatc aaaccctggc acttgctggc
    12601 tgatgatgtc gttttcttcg atcatcaact gcataccacc aagaaaacac ggtgccacgt
    12661 tgtcgtaatg aatgctgccg gagattcgtc cttccagctc gcccatcaaa gccagcaaac
    12721 gggtgtcatt aagcggcttg ccgcagtgtt cattcatcgc catcagagcc gcgaccaccg
    12781 aacaggcgct ggagcctaag cccgaaccga tcggcatatt cttttccaga gtcatcgcca
    12841 ctggaatttg cttgcccagc tcctggcaaa aacgctccca gcactgataa acgatatttt
    12901 cccgtggttc tgacggcagc ttatcggcaa agcgtccgag gttgttgaga ctgaatgtct
    12961 ctgccgactc aaccgtgact acatctccga gcaatgcacc atcaacgggt gtcaccgccg
    13021 ccccgagcac atcaaacccg acgctcatat tggcactgga agccggggca taaactttaa
    13081 ccatgtcaga ctcctaactt ccatgagagg gtacgtagca gatcggcaaa gacaccggca
    13141 gcggtaacgt cattgcccgc accatatccg cgcagcacca acggcagcgg ctgataatag
    13201 tggctataaa aggccagggc gttttcgcca tttttcactt tgaacagcgg atcattacca
    13261 tccacttcgg caatcttcac gcggcagacg ccatcttcat caatattgcc aacatagcgc
    13321 aaaacttttc cttcatcacg ggccttcgcc acgcgcgcgg caaagagatc gtcgagctgt
    13381 gacagattcg ccataaaagc ggcaacatca ccctcagcgt taaactctgc gggcagcaca
    13441 ggttcaattt caatatccgc cagctccagt tcacgtcccg tttcacgagc gagaattaat
    13501 agtttacgcg ctacatccat accagaaaga tcatctcgcg gatccggttc ggtataaccc
    13561 atttcccgcg ccagcgtagt cgcctcggag aaactcatgc cttcgtctaa cttgccgaag
    13621 atataagaaa gcgaacctga aagaatgccg gagaacttca tcaattcatc accagcattg
    13681 agcagatttt gcaggttctc aataaccggt aatccagccc caacgttggt gtcatagagg
    13741 aatttacgcc gcgatttttc agccgcatga cgcaacagat ggtagtaatc catcgacgag
    13801 gtgttggcct ttttgttcgg cgtgacaacg tggaaacctt cgcgcaggaa gtcggcatat
    13861 tgatccgcca ctgcctggct ggaggtgcag tcaacaatga ccgggttcag cagatgatat
    13921 tctttcacga ggcgaattaa gcgcccgaga ttaaacggct ctttggcttg cgccagttct
    13981 tcctgccagt tttccagatt taggccatgc acattggtga gcagagcctt cgagttagca
    14041 acaccgcaga cacgtaagtc gatatgttta ttcttcaacc agctttgctg acgcttcagt
    14101 tgctccagca gcgcaccgcc aacgccaccg acgccaatca caaacacttc gataacctga
    14161 tcggtattga acagcatctg atgagtaacg cgcacgccag tggtcgcatc atcgttattt
    14221 accacgacag agattgagcg ttcagaagat ccctgagcaa tagcgacaat gttgatattg
    14281 gcgcgggcca gcgcggcaaa gaatttcgcc gagatcccac gcaaggtgcg cataccatca
    14341 cctaccaccg agataatggc cagccgttcc gtcaccgcca gcggctccag taagccttct
    14401 ttcagttcca ggtagaactc ttcctgcatt gcccgttcag ctcgcacaca gtcgctttgc
    14461 ggaacgcaga aactgatact gtattcggaa gatgattgcg taatcagcac cacggaaata
    14521 cgggcgcgtg acattgcagc aaagacgcgc gccgccatgc cgaccattcc tttcatcccc
    14581 gggccggaaa cgctgaacat tgccatatta ttcagattgg aaatgccctt gaccggtaat
    14641 tcgtcttcat cacgactggc accaatgagc gtacctggag cttgaggatt tccggtattt
    14701 ttaatcaggc aagggatctg gaactgggcg atgggggtaa tggtgcgggg gtgaagaact
    14761 ttagcgccga agtaggaaag ctccatcgct tcctggtagg acatcgattt caacaacctc
    14821 gcatcgggca cctgacgcgg gtcgcaggta tataccccgt caacgtccgt ccaaatctcg
    14881 caacaatcgg cgcgtaaaca ggcagccagc accgccgcgg agtagtcgga accgttgcgt
    14941 ccaagtacca ccagttcgcc tttttcatta ccggcggtga aacctgccat cagcaccatg
    15001 tgatcagccg gaatacgact tgccgcaata cggcgggtgg actctgcaat atcgacagta
    15061 gattcgaggt aatgccccac tgccagcagt ttttcgaccg gatcgataac ggtaacgttg
    15121 tgaccgcgcg cttctaatac gccggccata atggcgatcg acattttctc gccacggcaa
    15181 atcagcgcag cgttaatgct atccgggcac tgccctaaca aactaatgcc atgcaggaca
    15241 tgttttattt gggcaaattc ctggtcgacg aaagttttca attgcgccag cgggaatccc
    15301 ggctgggcgg cggcgagtcc cgtcagaagt tcggcaaaaa tacgttcggc atcgctgata
    15361 ttgggtaaag catcctggcc gctaatggtt ttttcaatca tcgccaccag gtggttggtg
    15421 attttggcgg gggcagagag gacggtggcc acctgcccct gcctggcatt gctttccaga
    15481 atatcggcaa cccgcagaaa acgttctgca tttgccactg atgtaccgcc gaacttcaac
    15541 actcgcatgg ttgttacctc gttacctttg gtcgaaaaaa aagcccgcac tgtcaggtgc
    15601 gggctttttt ctgtgtttcc tgtacgcgtc agcccgcacc gttacctgtg gtaatggtaa
    15661 tggtgatggt ggtggtgatg gtggtggtaa tggtggtgct aatgcgtttc atggatgttg
    15721 tgtactctgt aatttttatc tgtctgtgcg ctatgcctat attggttaaa gtatttagtg
    15781 acctaagtca ataaaatttt aatttactca cggcaggtaa ccagttcaga agctgctgtc
    15841 agagactctt tttttaatcc acacagagac atattgcccg ttgcagtcag aatgaaaagc
    15901 tgaaaaatac ttactaaggc gttttttatt tggtgatatt tttttcaata tcatgcagca
    15961 aacggtgcaa cattgccgtg tctcgttgct ctaaaagccc caggcgttgt tgtaaccagt
    16021 cgaccagttt tatgtcatct gccactgcca gagtcgtcag caatgccatg gctcgttcgc
    16081 gtaaagcttg cagttgatgt tggtctgccg ttgtgtcact tttcgtcggt tgttgtatta
    16141 atattgctaa ttgatagcaa tagaccatca ccgcctgccc cagattgagc gaaggataat
    16201 ccgccaccat cggcacacca gtaagaacgt cagccaacgc taactcttcg ttagtcagcc
    16261 cggaatcttc gcgaccaaac accagcgcgg catggctcat ccatgaagat ttttcctcta
    16321 acagcggcac cagttctact ggcgtagcgt agtaatgata tttcgcccga ctgcgcgcag
    16381 tggtggcgac agtgaaatcg acatcgtgta acgattcagc cagtgtcggg aaaactttaa
    16441 tattatcaat aatatcacca gatccatgtg cgacccagcg ggtggctggc tccaggtgtg
    16501 cctgactatc gacaatccgc agttcgctaa accccatcgt tttcattgcc cgcgccgctg
    16561 ccc
//
LOCUS       DBFIFM010000102        16465 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.102, whole genome shotgun sequence.
ACCESSION   DBFIFM010000102 DBFIFM010000000
VERSION     DBFIFM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.102"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..182
                     /locus_tag="N7C74_002781"
     CDS             <1..182
                     /locus_tag="N7C74_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU2432615.1"
                     /translation="DKQYVWCWGKSYGWTECDTFEGYYDCSRNKWWAVTDNGEEPASK
                     VTHWMPLPEPPQEVK"
     gene            270..512
                     /locus_tag="N7C74_002782"
     CDS             270..512
                     /locus_tag="N7C74_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU2432616.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRSTQHSVFFRRMDGRSGRVRIG
                     EFNNLFEHIDQQEYRKILAGTEQEMRLKKLRAMQRR"
     gene            516..650
                     /locus_tag="N7C74_002783"
     CDS             516..650
                     /locus_tag="N7C74_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432617.1"
                     /translation="MHTAFEFWVRKTFGNRYDLNRDVDGFYCREVVKRMFDVWCHCRG
                     "
     gene            669..923
                     /gene="xisR"
                     /locus_tag="N7C74_002784"
     CDS             669..923
                     /gene="xisR"
                     /locus_tag="N7C74_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGU2432618.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            957..2243
                     /locus_tag="N7C74_002785"
     CDS             957..2243
                     /locus_tag="N7C74_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2432619.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEICAN
                     ALNRYQSVIKNMLPMLGEKKLVSSITKEDLLFVRRDLLTGYQKLSNGKTSSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLAPLKKSKVKPDPLTRDEFIRFIEACRHQ
                     QTKNLWILAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRTAVHKCTFVFSPQVTKQQQLSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKAG"
     gene            2264..2965
                     /gene="mlrA"
                     /locus_tag="N7C74_002786"
     CDS             2264..2965
                     /gene="mlrA"
                     /locus_tag="N7C74_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HGU2432620.1"
                     /translation="MSLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQYQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            3025..3132
                     /gene="yohO"
                     /locus_tag="N7C74_002787"
     CDS             3025..3132
                     /gene="yohO"
                     /locus_tag="N7C74_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HGU2432621.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(3113..3844)
                     /gene="yehW"
                     /locus_tag="N7C74_002788"
     CDS             complement(3113..3844)
                     /gene="yehW"
                     /locus_tag="N7C74_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HGU2432622.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(3849..4775)
                     /gene="yehX"
                     /locus_tag="N7C74_002789"
     CDS             complement(3849..4775)
                     /gene="yehX"
                     /locus_tag="N7C74_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HGU2432623.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGELRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPSGTLHFQDLLVEA"
     gene            complement(4768..5925)
                     /gene="yehY"
                     /locus_tag="N7C74_002790"
     CDS             complement(4768..5925)
                     /gene="yehY"
                     /locus_tag="N7C74_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HGU2432624.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLASALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAGLVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(5932..6849)
                     /gene="osmF"
                     /locus_tag="N7C74_002791"
     CDS             complement(5932..6849)
                     /gene="osmF"
                     /locus_tag="N7C74_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HGU2432625.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLHAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLRRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAAQGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(7101..9398)
                     /gene="bglX"
                     /locus_tag="N7C74_002792"
     CDS             complement(7101..9398)
                     /gene="bglX"
                     /locus_tag="N7C74_002792"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HGU2432626.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITILQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            9594..11309
                     /gene="dld"
                     /locus_tag="N7C74_002793"
     CDS             9594..11309
                     /gene="dld"
                     /locus_tag="N7C74_002793"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HGU2432627.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGHG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(11347..12279)
                     /gene="pbpG"
                     /locus_tag="N7C74_002794"
     CDS             complement(11347..12279)
                     /gene="pbpG"
                     /locus_tag="N7C74_002794"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HGU2432628.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(12453..13040)
                     /gene="yohC"
                     /locus_tag="N7C74_002795"
     CDS             complement(12453..13040)
                     /gene="yohC"
                     /locus_tag="N7C74_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HGU2432629.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGDMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            13210..13788
                     /gene="yohD"
                     /locus_tag="N7C74_002796"
     CDS             13210..13788
                     /gene="yohD"
                     /locus_tag="N7C74_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGU2432630.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGLAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(13918..14679)
                     /gene="yohF"
                     /locus_tag="N7C74_002797"
     CDS             complement(13918..14679)
                     /gene="yohF"
                     /locus_tag="N7C74_002797"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU2432631.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGAQALEKLIQRLGRIDVLVNNAGAMTKVPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(14732..16168)
                     /gene="mdtQ"
                     /locus_tag="N7C74_002798"
     CDS             complement(14732..16168)
                     /gene="mdtQ"
                     /locus_tag="N7C74_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HGU2432632.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLIALTNHQTKSLTLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
BASE COUNT         4060 a         4428 c         4149 g         3828 t
ORIGIN      
        1 acgataaaca gtatgtttgg tgttggggga agtcttacgg ctggactgag tgcgatacct
       61 tcgaagggta ttacgattgt tcgagaaaca aatggtgggc agttactgac aatggggaag
      121 aaccggcatc gaaagtaacc cactggatgc cgctaccgga gccgccgcag gaggtgaaat
      181 gatgaattgg cctgaagcat tcaccgcagt tggagttgca atagcggtgg catttattct
      241 gtattcgctt ttccgctggg gataaaggaa tgttcgctct gattcaacgt ggtcagatat
      301 acacggacag agccggatac cctgtggtga ttactcgcag tactcagcac tcagtgttct
      361 ttcgacgcat ggacgggcgc tccggacggg tacgcattgg tgagttcaac aacctgttcg
      421 aacatattga ccaacaggag taccgcaaaa ttctggcggg cactgagcag gaaatgcgcc
      481 tgaaaaaatt acgcgcaatg caacggaggt gatacatgca tacggctttt gagttctggg
      541 ttcgcaagac attcggcaat cgctacgacc tgaaccgtga tgtcgacggc ttctactgcc
      601 gtgaagttgt gaaacgaatg tttgacgtgt ggtgccactg ccgtggatga aagttttatg
      661 aggttggcat gcagacaatc atctatcaga taacccccag caaatggtgt acggagagag
      721 tccttattgc atcaacaggg ctaaagcccg gcaccatcga gcgggccaga agaaagtcat
      781 ggatgcaggg aaaagaatac cgccattacg ctgtagaagg tgatcctggg cattacagtg
      841 aatgcctgta caacatcgaa gaaattatgc gatggatcga aaaccagaaa caaccaggtg
      901 ccaaaaatgc aagttccggt taacctgtta atgctcctgg acgtctggga ggtttaatga
      961 gtaacgcatc atacccgaca ggcgttgaaa accatggcgg atcactccgt atatggtttc
     1021 actataatgg caaacgtgtc agagaaaacc tcggtgttcc tgacacagcc aaaaaccgga
     1081 agatcgctgg tgaacttcgc acttccgttt gttttgcaat cagaatgggg agtttcgact
     1141 acgccgcgca gttccctaat tcccctaacc tgaaacactt tggtctggga aaaagagaga
     1201 taaccgttaa ggcactttcg gaaaaatggt tggaccttaa gaaaattgag atttgtgcga
     1261 atgcacttaa ccgttaccag tcagtaatta aaaacatgtt accaatgtta ggtgaaaaaa
     1321 aactggtttc atccataaca aaagaggatt tacttttcgt aaggagagat ttgttgaccg
     1381 gttaccaaaa gctttctaat ggaaagactt cttccataaa agggcgctca gtggtcacgg
     1441 taaactacta tatgacaacc atagctggaa tgtttcaatt tgcaacagat aatggttata
     1501 cctcaggaaa cccatttaac ggtctggctc ccttaaaaaa gtccaaggta aaaccagatc
     1561 ctctcacccg tgacgaattt attcgtttta ttgaggcttg ccgtcatcaa caaacaaaaa
     1621 acctgtggat tctcgctgta tacacgggta ttcgtcacgg ggagttggta tcgctggcat
     1681 gggaagatat agaccttaaa gcaaggacta taaccatccg tagaaattat acaaaacttg
     1741 gcgaattcac tccaccaaaa accgatgcag gcaccggaag gacaattcat ctggttcaac
     1801 cagctattga tgctcttaaa agccaggcgg aaatgaccat gcttggaaag caacattctg
     1861 tagaggtgaa gcagagggaa tatgggagaa ctgctgtgca taaatgcact tttgttttta
     1921 gtcctcaggt aacaaaacag cagcagttgt ccggacctca ctacaaggtt gactccatca
     1981 gggagtcatg gacaagtatc ttaaaacgcg caggtctgag acacagaaaa tcgtaccaat
     2041 ccaggcatac ttatgcatgc tggtcacttg ccgcaggagc taatcctagt tttatcgcaa
     2101 gccagatggg ccacacaaac gcacaaatgg tattcaatgt ttacggagca tggatgaaag
     2161 acaacaatca cgaacagata gaactcctta acaaaagact atctgaaagt gtcccatgta
     2221 tgccccataa gaaagcaggg taaaataaaa acttgcaaaa tcaattagtt tacccttaat
     2281 ccctgtcacg ttacgcgcgt ggcagaggcg ttacggattg ctgaaaccgc aacggacaga
     2341 cggcggtcat cggctgttca acgatgccga tattgaccgg atccgcgaga tcaaacgctg
     2401 gatcgacaac ggcgtgcagg tcagcaaagt taaaatgctg ctcagtaatg aaaatgttga
     2461 tgtgcagaac ggctggcgcg atcagcaaga aacattactg acctacctgc aaagcggcaa
     2521 tctgcatagc ctgcgaacgt ggatcaaaga gcgcggtcag gattaccccg cccagacgct
     2581 caccacacat ctgtttattc ctctgcgccg acggcttcag taccaacaac cgactctcca
     2641 ggcgctgctg gcgatcctcg acggcgtact gatcaactac atcgccattt gtctggcttc
     2701 ggcacgtaaa aaacagggta aagatgcgct ggtggttggc tggaatattc aggataccac
     2761 ccgtctgtgg ctggagggct ggattgccag tcaacaagga tggcgcattg atgtcctcgc
     2821 ccactcgctc aatcaactac gccctgaact attcgaaggc cgtacattgc tggtgtggtg
     2881 cggtgaaaat cgaacctccg cccaacagca gcaactcacc agttggcaag aacaaggcca
     2941 tgatattttc ccactcggca tttaatgatt cgttaacaaa tgcgctttac tgtacaatcc
     3001 tttcgttaac ataaggagtg cattatgcgc atagctaaaa ttggggtcat cgccctgttc
     3061 ctgtttatgg cgttaggcgg aattggtggc gtcatgctcg caggttatac ctttattttg
     3121 cgtgctggct aagcgcctgc accagccttt caaacaggcg gtctgcgatg atcgccgcca
     3181 gtgccaccag taacgccccc tggatcacat acgcggtatt aaatccgcta agcccgatga
     3241 tgatgggcgt acccagcgtg ctggccccta ccgttgaggc gatcgtcgcc gtaccaatgt
     3301 tgataatcac cgaggttcgc acgcccgcca gaatcaccgg agccgccagc ggtagctcga
     3361 ccttacgcag tcgctgacct cgactcattc ccataccttt cgcaacttct gtcacgctgg
     3421 catcaatcgc tcccagcccg gcaagtgtcg cctgcaggac gggcagcaca ccataaagga
     3481 tcaaggcgat aatcgctggt tgcagaccaa agcctatcac cggaacagca atcgccagca
     3541 ctgcgacagg cggaaaagtc tgcccaacgg cggcaatagt ttccaccagt gggcgaaatt
     3601 ccgcgcccca cgggcgagtg actgcaattc cggcaccagt gccaatgatc accgcaaaca
     3661 aactcgaaat tcccaccagc cagaaatgag ccagtgccaa agctgcaaaa ctttcttgct
     3721 gataaacggg tcgtggcagt tgtgggaaca aggcagcaaa cagcggctgg ctgtaaggca
     3781 gccagaaaat cagtgccaca aacaaagcaa tgagccagaa cagcggatcg cgcaacatct
     3841 tcatacgctt acgcctccac cagcagatcc tgaaaatgca gcgtgccgct aggctggccc
     3901 tgcgtgttca ccaccggcag cacctcgcat ccccgcgcga caaacaggga gagcgcatcg
     3961 cgcagcgtca tctcttctgc cagtgcctca ccttctgccc gttcttcgcg acgcacgtaa
     4021 tccgccacac tacgtaacga aagcaggcgc acacccagtt cactacgtcc aaaaaactgg
     4081 cggacaaaat cattcgccgg acgagtcagc atcgtcagcg gattcccctg ctgcaccact
     4141 tcaccgtgat ccatcaatac cagatgttct gccagccgta gcgcctcatc aatatcatgc
     4201 gtgaccagca caatggtacg ccccagcaaa cggtgaatgc gcgtcatctc ttgttgcaac
     4261 gcgccgcgcg ttaccgggtc cagtgcgcca aaaggttcat ccatcagtaa gacttgcgga
     4321 tcggcagcca gtgcgcgcgc tactcccaca cgttgctgct gaccaccgga aagctgatgt
     4381 ggataacgct cacgtaaatt tgactccagc cccagtagcg ccattaattc gtcgatacga
     4441 tcgtcaatcc tcgcccgcga ccatttttgt aattgcggca cggtggcaat gttttgtgcc
     4501 acgctccagt ggggaaacag gccaatagat tgaatggcat agcccatacg gcggcgcaac
     4561 tccagtactg gcagcgagcg aatttcttct ccagcaaagc ggagctctcc gctgtcatgt
     4621 tccaccaggc ggttaatcat tttcagggtg gtggatttgc cggagccaga tgtgccaatc
     4681 agcaccgaaa aactcccttc ctgaaaattg agattgagat cgttaacggc tttttgtgcg
     4741 ccgaacagtt tgctgacatg gctaaattca atcattacgt ttcaccttca acagtgcgat
     4801 tagtaaatcg aacagcgcat cgatcagcac cgccagaaca attaccggga tcacccccag
     4861 caacactaaa tcaatggcgc tgcttagcag cccctggaaa accagcgcac caaaaccgcc
     4921 tgcgccgatt aacgccgcaa tcaccgccat gcctacagtt tgcaccatca ccacccgcag
     4981 gctgcgcaga aataccggta acgccagcgg taactgaaca tgcaggaatc gctgcgcccc
     5041 gctcatgccc atcgctctgg cgctctccag cacatcgcgc gggatctggt tcaagccgac
     5101 taccacgccg cgcaccagcg gtagcaaggc atagagcacc agcgcaatca gtgcgggtgt
     5161 cattccggtt cctgctatgc caagcttccc cagccacgga aaggccgtaa ccagtccggc
     5221 aagcggcgca atcagcaggc caaagagcgc cacggaaggc acggtctgaa tgacattgag
     5281 cagagaaaaa atcgccccct gccgcgcggt ggaaaagtag caccagatgc ccaacggcac
     5341 accaatcact aacgcaggca gcaccgcacc aaacagcaac gtcagatgtt gtgccagcgc
     5401 gtcgtcaaac acatcctgac ggttggcgta ttctttcatt agtgagagat cgttaagcgt
     5461 gccggagtac agcaaccaca gcggaataat ggcaatctgc atatgcaaca accagcgcca
     5521 cagcggatgc gtggagattc ggcggatggc atcgctacag gccagcaatg ccagcgccga
     5581 agccaaccag aaaccactgc cgaggctggt acgcgccagc gcactgccat tttgcgccag
     5641 ttgggtcgcc gcctttccag ctccccacac cagcaatacg aagacgaatt gcgccagaat
     5701 gagtgcaaaa atgctgcctt ttttagcggg aataaaacag gctgtcagcc aggcgcaacc
     5761 aacgcccacc agcatccaga tcgtttgcgg ccacagttgc cagagatgac gcccttcacc
     5821 cgagactaaa cgattaggcg cgtagctgat aaacggcagc gctgctgcga ttgccgtcag
     5881 caacagcagc agcgccagaa caggattaat acgtagataa gtcacgggaa attacttcgt
     5941 ccaccctttt tgtttcaagt agtcggcagc cacttttttg gcatccagtc cttccactgc
     6001 aatgctggca ttcagttgct gcaatgtttt tgcatcgagg ctggcgaaga ctggctgtag
     6061 ccactgtgcc atttgcggat actctttcag caccgactca cgcaccactg gtgcaggcgc
     6121 gtagataggt tgcacgcctt gcggatcgct taaggtttgc agcccctgcg ccgcgaccgg
     6181 accgtcagtg ccgtaagcca ttgcggcatt aacgccagaa gtttgctggg cagcggcttt
     6241 aatcgttacc gccgtgtcgc cgccagccag tgacagtaac tgatcctgat cgagtttaaa
     6301 gtcgtaggct ttttcaaacg cgggtaacgc atcggcgcgt tcgataaact cagccgaggc
     6361 tgccagtttg aaggtgccgc cctctttcag ataacgcctc aggtcagcaa gcgaagtgag
     6421 tttgtttttc tctgccacat cctgacgcac ggcgatggtc caggtgttat ttgcaggtgc
     6481 gggcgtcagc cagattaact tgttttgctc tgcatcgagt tttttgactt tctcgtaacc
     6541 ttgctgcgcg tttttccacg ctgcatcgtt ttcatcttta aagaaaaacg cgccattgcc
     6601 ggtatattcc ggatagatat ccagttcacc ggaagtaatc gccccgcgca ccacaggagt
     6661 cgttccaagt tgcactttat tgaccgttgg tactccgtgg ctttcgagta cttgcaaaat
     6721 gatattgccg agcagcgcgc cttcggtatc gatttttgaa ccgactttaa cgggggaagc
     6781 cgcgtgcagc ggcaggctca cggctgccaa caaaaccagt gaacctgccc agacctttga
     6841 gagtggcatg atgctttcct cattctttta ctgttgtttt cagcgaatta agagaaaagc
     6901 atagttgata atggcgggat tagcctgagc ggacggattc agttgcagaa tcagataaat
     6961 acttaagagg catattcggt ccggcgtttc ctgccggatg cggcgcgagc gccttaaccg
     7021 gcctacaaag ggcgaaaatt cagcaagtta agatatgaac atcaggcaaa gggcgcaaac
     7081 gtcgcgccct tgctaaagca ttacagcaac tcaaactcgc ctttcttaac gcgtgcggaa
     7141 tcagtgccga taaagacatt gaacttgcca ggctcggcgt catatttcat ctgttgattc
     7201 cagaacttca gcgcctcgat atcaatcggg aagctgacgg tctgggtttc gcccggcttc
     7261 agggtgattt tctcaaagcc tttcagctgc ttcactggac ggctcatgga agccgtcaca
     7321 tcctgcaagt acatctgcac taccgtggcc ccttcgcgtt taccggtgtt cgtcacctgc
     7381 acgctggccg tcactttgcc gtcacgcttc atggtcggcg cagaaagttt cacatcagag
     7441 acggtgaaag tggtatagct cagaccatag ccgaacggat aaagcgcccc gttagcttca
     7501 tcaaaataac gcgaagtgta tttgttcggc ttgtcggcat tatacggacg accggtgttc
     7561 agatggctgt agtacaccgg gatctgcccg acagaacgcg ggaaggacat cggcagctta
     7621 ccggacgggt tgtaatcgcc aaacaacaca tcggcaattg cattaccgcc ttcagtcccg
     7681 gcaaaccagg tttccagaat cgcatcagcc tgctgatctt ctttcaccag cgccagcgga
     7741 cgcccgttca tcagcaccag caccagcggt ttgccggtgg ctttcagggc ggcaatcaaa
     7801 tcacgctggc tttgcagaat agtaatatcg gtacggctgg aggcctcgtg cgccatcccc
     7861 tgtgcttcac cgactacagc caccaccaca tcagattgtt tcgccgtctg caccgcttca
     7921 tcaatcatct cttgcggcga acgcggatcg actttgaccg cttcttcata ctgattcagg
     7981 aaatcgataa tgcctttgtc actggtaacg ttcgcccctt tggcatacag cactttaccg
     8041 ttttcaccga cggcattttt aatcccggtc agcacggtca cggattgatc ggcaacacct
     8101 gccgccgacc agctgcccat cacgtcacgt ttactgtccg ccagtggccc aaccaccgca
     8161 atggtggctg attttttcag cggtaacgtt tcgagacggt ttttcagcaa caccaggctt
     8221 tcacgcgcca cttcacgcgc ttctttgcgg tgcaggcggc tttcggcatt ggtatccacc
     8281 gggtcagact ctttcggacc gagatggctg tatgggtcgt taaacaaccc catatcatat
     8341 ttaacgttca gtacatggcg ggcagcatcg tccagctctg ccatcgtcac tttaccggat
     8401 ttgatcaacc caggcagata cttcgagtag tactcgtcgc tcatgctcat gttgattccg
     8461 gatttcagcg ccacgcgcac tgcatcttcc ggatctgccg ccgtgccatg tttaatcagc
     8521 tctttgattg caccgtgatc ggaaacggtg atgcctttaa agccccactg gtcgcgcaga
     8581 acatctttca gcagccagga gtcggaggtg gctggcgtgc cgttcagcga gttcagcgcc
     8641 accatcaccg cgccgctgcc tgcgtccagc cccgctttgt acggcggcat ataatcatta
     8701 aacaggcgct gcggactcat atcgacggtg ttgtactctt taccgccttc taccgcgccg
     8761 tatgcggcaa agtgtttgac gctggtcatc accgagtaac gatctgccgg gcttttaccc
     8821 tgcatcgctt ccaccatggt tttacccatt attgaggtga gatacgtatc ttcgccaaaa
     8881 ccttcggaag cacgtcccca gcgcggatcg cgcgagacat cgaccatcgg tgcccaggtc
     8941 atattcaggc catcatctgc cgcttcataa gcagagacac gtccgaccgt ttttactgca
     9001 tcgaggttaa aagacgaggc cagaccgagg ctaatcggga acaccgtgcg ctgaccgtgc
     9061 agcacgtcgt aagcaaagaa aagaggaatt ttcaggcggc ttaattccat cacctgatcc
     9121 tgcatggcgc ggatatcctg acgggttacg gtgttgaaaa tagccccaac ctgaccgtct
     9181 ttgatcatct cgcggatcgc ctctttcggg ttatccgggc cgacgctgat taagcgcagc
     9241 tgaccaattt tctcatcaac tgtcattttc ttaagcagtt cggtgacgaa cgcatcccgc
     9301 gcttcaggcg ttaatggatg gttaccgaat aaatcatccg ccagtgctgg ctgcagggcc
     9361 agactcaccg cgattcctac tgaacatagc catttcatat ggatttttct tcctttgttg
     9421 cccgacgtgg cagcgaaaat ggtgcaaaaa ccgtagtttg ccataagcat gatggagaga
     9481 gaaaaagaat gctcagttta ttgtctgaat tttcaaaata ttcactcgct gaattgtcat
     9541 acaaggcgct attctagttt gtgatgtttt ttcgccacca caaggagtgg aaaatgtctt
     9601 ccatgacaac aactgataat aaagcctttt tgaatgaact tgcccgtctg gtcggtcatt
     9661 cacacctgct caccgatccc gcaaaaacgg cccgctatcg caagggcttc cgttctggtc
     9721 atggcgacgc gctggctgtc gttttccctg gctcactact ggaattgtgg cgggtgctga
     9781 aagcctgcgt caccgccgac aaaattattc tgatgcaggc tgccaataca ggcctgaccg
     9841 aaggatcgac gccaaacggt aacgactatg atcgcgatat cgtgatcatc agcaccctac
     9901 gtctcgacaa gctgcacgtt ctcggcaagg gcgaacaggt gctggcctat ccgggcacca
     9961 cgctttattc cctggaaaaa gccctcaaac cgctgggacg cgaaccgcac tcggtgattg
    10021 gttcatcgtg cattggcgca tcggttatcg gcggtatctg taataactct ggtggttcgc
    10081 tggtgcagcg tggcccggcg tataccgaaa tgtcgttatt cgcgcgtata aatgaagatg
    10141 gcaaactgac gctggtgaac catctgggga ttgatctggg cgaaacgccg gagcagatcc
    10201 ttagcaagct ggatgacgat cgcatcaaag atgacgatgt gcgtcacgat ggtcgtcacg
    10261 cccacgatta tgactatgtc caccgcgttc gtgatattga agccgacacg cccgcacgtt
    10321 ataacgccga tccggatcgg ttatttgaat cttctggttg cgccgggaag ctggccgtct
    10381 ttgcagtacg tcttgatacc ttcgaagcgg aaaaaaatca gcaggtgttt tatatcggca
    10441 ccaaccagcc ggaagtgctg accgaaatcc gccgtcatat tctggctaac ttcgaaaatc
    10501 tgccggttgc cggggaatat atgcaccggg atatctacga tattgcggaa aaatacggca
    10561 aagacacctt cctgatgatt gataagttag gcaccgacaa gatgccgttc ttctttaatc
    10621 tcaagggacg caccgatgcg atgctggaga aagtgaaatt cttccgtccg cattttactg
    10681 accgtgcgat gcaaaaattc ggtcacctgt tccccagcca tttaccgccg cgcatgaaaa
    10741 actggcgcga taaatacgag catcatctgc tgttaaaaat ggcgggcgat ggcgtgggcg
    10801 aagccaaatc gtggctggtg gattatttca aacaggccga aggcgatttc tttgtctgta
    10861 cgccggagga aggcagcaaa gcgtttttac accgtttcgc cgctgcgggc gcagcaattc
    10921 gttatcaggc ggtgcattcc gatgaagtcg aagacattct ggcgctggat atcgctctgc
    10981 ggcgtaacga caccgagtgg tatgagcatt taccgccgga gatcgacagc cagctggtgc
    11041 acaagctcta ttacggccat tttatgtgct atgtcttcca tcaggattac atcgttaaaa
    11101 aaggcgtgga tgtgcatgcg ttgaaagaac agatgctgga gctgctacag cagcgcggcg
    11161 cgcaataccc tgccgaacat aacgtcggtc atttgtataa agcaccggag acattgcaga
    11221 agttttatcg cgagaacgat ccgaccaaca gtatgaatcc ggggatcggt aaaaccagta
    11281 agcggaaaaa ctggcaggaa gtggagtaaa aattacggat ggcagagtat cgccatccga
    11341 attcacttaa tcgttctgtg ccgtctgccc cgccgccgcc atttgggcgg ctttttgttt
    11401 tttatagctt aacgctgctg ccggaacagg catcacttta ccggtttcaa tccaggtacg
    11461 caggcggctg gcatcggcaa aatgggtgta tttgccaaac gcatccatca ctaccagcgc
    11521 caccggttta ttattgataa ccgtacgcat caccagacaa tggcccgccg cattggtaaa
    11581 gccggttttg gttagctgga tattccagtt atcgcgatac accagatgat tagtattgcg
    11641 gaacggcagc gtatacgtcg ggttagagaa ggttgccata tcttcccggg tagtacttaa
    11701 ctgcccgatc aacggatatt gtttgctggc aatgagcagt ttggttaagt cacgggcggt
    11761 tgaaacgtta tgcaccgaca atccggtagg ttcaacaaag cgcgtgttgt tcattccgag
    11821 cgatttcgct ttcgcattca ttgccttaat aaaggctttg taaccaccgg gataatggtg
    11881 cgcaaggctt gccgccgcgc ggttttctga agacatcagc gccagcaaca gcatatcttt
    11941 acggctgatt tcgctattca gtcgtacgcg cgaatagacc cctttcatct ccggcgtctg
    12001 gctgatatcc acttttagtt tttcatccag cggcagtcgt gcatccagca cgaccatcgc
    12061 agtcattaat ttgctgatag acgcaatcgg acgcaccaga tccgggtggt tcgaatagat
    12121 cactttgttg gtattcagat caacaatcat cgcgctaccg gaggcaattt ccggttgtga
    12181 agcggtggta gcggctgccg ttttcgcaac ggcctgcggt gccaaaggca cagccagcat
    12241 cagggccagg ctaaataaag aaactcgaaa tttcggcatg atgagcattc agatagtggt
    12301 tcacgcgcac gggttgcgca ccgccggagt aaggatttac tgaggctagc gacgccatca
    12361 taacgagcaa aaagtgcgat cgtcaaagga gaatcgtgag gaaatgctgc attgctgaca
    12421 tttacgccag caatgcaacg tcaaagaact ttctagaaca aacgataacc gtagccccat
    12481 agtataacgg taagggcgag cagcacttcc agtaccagca cgccaatcgc cagcgtcgaa
    12541 ctggagaagc taaggccttc ctctttgttg atattcagga agctcggaat accaaggtaa
    12601 agtaggtaac cggtgtaaaa cagtgccacc gtgccgacca gcgcacacaa ccagaccagc
    12661 ggataaagcg ccaccagacc acttaaaaac agcggagttg caacgtagcc cgcgaagacc
    12721 atgcagtgcg caagtgacgg acgctgcgga taattacgcg ccatccacca gatgacccgc
    12781 cccatcaccg cgaccccagc gagcatatcg ccataaaaca ggacagccag cgccagtccg
    12841 gtaaaccagg ataacttcag gatagtgcca tcgccaaaat tccagccaat ctgtgtagtg
    12901 ccaatgaagg cgcaaatcac cggaatcgcc gccatcagca aaacgtggtg ggtgtaatgg
    12961 tgagaaatcg tttcgttttc gcgattaatc acctgcattt cacgatcggg atgggaaaac
    13021 agtccccaga catggctcat accgccccct tgttgtgagt tcatgaacct gacagttcaa
    13081 gtataagtca gcttgtgatt attttttgtt cgcccatgta atttgcaccg tctgatgtac
    13141 gtcctttttc agcgtgaatg attcacaggg tgtatgctta caggcaacca aagggagaca
    13201 gactggccta tggatctcaa tacactcatc tcacaatatg gttatgccgc gctggttatc
    13261 ggtagcctgg cggaaggtga aaccgtgact ttgctgggag gccttgcggc acatcagggg
    13321 ctattaaagt tcccgctggt ggtactttct gtagcgcttg gcggcatgat tggcgaccag
    13381 gtgctctatc tgtgcgggcg gcggtttggc ggtaagctgt tacgccgttt ctctaaacat
    13441 caggataaaa ttgagcgggc gcagaaactg atccaacgcc atccgtatct gtttgtcatt
    13501 ggtacgcgct ttatgtatgg ctttcgggtg attggcccga cgctgattgg tgccagccag
    13561 ctgccgccga aaatctttct gccgcttaat attctcggcg catttgcctg ggcgttgatt
    13621 tttaccacta tcggttacgc tggtggtcag gtgattgcgc cgtggttgca caatctcgac
    13681 cagcatttga agcactgggt ctggttgatt ctggtcgtgg ttctggtggt aggcgtgcgc
    13741 tggtggctga aacgacgcgg gaagaaaaag ccggataatc aggcgtaaaa ccattgccct
    13801 ggataaggcg tgcgcgccgc atccgacatc cggttcacca tgcctgatgc ggcgcttgtg
    13861 cgtcttatca tgcctaccaa aattttaccc attgaaatat ccacacacct cactgcgcta
    13921 ttctgggttg aactgcggat tcgccaacat aaagccgcca tccactatca acgactgccc
    13981 ggtggtgtaa tttgcgccct ccgaacaaag ccacgccacc aggctggcaa tctcatgcgt
    14041 tgcgccaaaa cgccgcagag gaatcgaagg ctccgcgtcg ggcttcacgt cgctatcatc
    14101 catgccgttc attggcgtag cgatcgcccc aggtgcgact gcgttcacca aaatcttatg
    14161 cctgaccaac tccagcgcca ttgctttggt taacccgccg agcgcatgtt tagcggctgt
    14221 gtaggcgctg gcatccggca gcggcgtatg ttcatgtacc gacgtaatgt tgatgatgcg
    14281 accaccctgt ccttgtttca ccatctggcg ggcagcaatt tgcgagcata aaaatgcacc
    14341 atcgacatca acggtaaaaa tcttgcgcca ctcatcgaaa gccatatcaa gaaacggcac
    14401 tttggtcatt gcgcccgcat tattcaccag cacatcaata cgccccagcc gttgaataag
    14461 tttctccagc gcctgcgcac cttctggcag attgccgaga tccagttgca cgatctccgc
    14521 acgtacgccg tggctaacta cctcacgcgc ggtatctttt gccccttctt catctgagtg
    14581 ccaggtaatg ccaatatcaa acccttgctg cgccagtaat aacgcgcact ctttgccgat
    14641 ccccgaatcg gaggcggtaa taatcgctac ctgtgccatc gagttctcca cttaacgctg
    14701 aataaacgtt aagtatagaa gtcacatatc atcagcgttt gtaacccccg cccaacgcac
    14761 cagtgagttg aatggaggca tccagccact gcccttgcaa taacaggcca ttggcccgct
    14821 cacgcagcgc ggggattctg gcttcgctga cgcgggaacc agcaatgatg cccgcgttaa
    14881 agcgggcctg cgcaagacct accacacgca aggcatcgcg ctcaatttgc gcctgatgct
    14941 ggtttttctc cgccagtgtc tgaacctgac tggctgcccg cgccacgtca ttcaccgctt
    15001 caaccaccgc tttgttgtag ctggcgatag acaagttgct ttcggctttt gcgatatcga
    15061 gattggcgtt aagacgacca ctatcgaaaa tgggtagcgt caggcctgcc gtaacgccca
    15121 tttgctgcgc ggaatgacgg aacagatcgc ttaagtgcaa cgcatcctgt tgcaggaagg
    15181 ccatcaggtt gatgtcagga taaaatgccg cttttgccgc atcaatggtg cttagcgatg
    15241 actcaacgta ccagtgcgcc gcctgcaaat ctgcccgccg ggccagtaag gagtacccca
    15301 gttcatcagg aagctggctt gccactttcg gcaacgccac cggtttaagc gtcaatgact
    15361 tagtctggtg atttgtgagt gcaattaacc gtgcctcaat aattttcatt ttccccgcga
    15421 catcgttgag ctgctgccgg gttttgctgg cattaatatc ggtttccaca ccttcaactg
    15481 aagaagtaat cccgttctga tatagctggc gatcggtcgc gataatggtg ttctgctctt
    15541 tttcgatttg ctgcaagacc gtgtttaacg ccgcctgggt ttgccactcc cagtacaggc
    15601 gggctacgct gccagccagc aattggcggg tttgctcgcg ttccgccgcc cgtgctttaa
    15661 ccgtacccag gcgggcagta acctccgccc gattctttcc ccagatatcg agatgccagc
    15721 ccgccgttaa gccaaaagtt ccgttggtgt accacggacc ggtcgtacct gcggccggat
    15781 cgttcagagc aaacggcccc attagccctt ctgccgacat tttttgccgc tccatatccg
    15841 cagaaaagtc gatctgcgga ccatcctgag tagcaactgc cttcgcctgg gcttcagcta
    15901 actgaatgcg ctgttcagcc acctgcatat ccggtgcgtt ctgtagtgca ttgttaatta
    15961 aggaagtgag ttgattatcg tgatactcca gccaccattg gctgtctggc caaccatttt
    16021 tcagcgccgt gggtaatgcg gtgtcaactt gtgcagcggg cgtttgctgg cttaacgcct
    16081 ggcgggtttc atgcataggc gcacacccgg ccagcatcag taacagcgga aaacaggcga
    16141 tggctggata aaaggaatca cgattcatgg gggaataatc aggtaagaaa aggtgcgcgg
    16201 agattaccgt gtgttgcgat atatttttta gtttcgcgtg gcaatacatc agtagcaata
    16261 aaacgacata tccagaaaaa tatacactaa gtgaatgata tcttccgatt tatcttaatc
    16321 gtttatggat aacggcaaag ggcttcgttt tttcctatac ttattcagca ctcacaaata
    16381 aaggaacgcc aatgaaaatt atactctggg ctgtattgat tattttcctg attgggctac
    16441 tggtggtgac tggcgtattt aagat
//
LOCUS       DBFIFM010000103        16222 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.103, whole genome shotgun sequence.
ACCESSION   DBFIFM010000103 DBFIFM010000000
VERSION     DBFIFM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.103"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            215..985
                     /gene="yjjP"
                     /locus_tag="N7C74_002799"
     CDS             215..985
                     /gene="yjjP"
                     /locus_tag="N7C74_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313351.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HGU2432633.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQHPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            976..1449
                     /gene="yjjB"
                     /locus_tag="N7C74_002800"
     CDS             976..1449
                     /gene="yjjB"
                     /locus_tag="N7C74_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HGU2432634.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLGGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1556..2095
                     /gene="dnaT"
                     /locus_tag="N7C74_002801"
     CDS             1556..2095
                     /gene="dnaT"
                     /locus_tag="N7C74_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HGU2432635.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVDLDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2098..2835
                     /gene="dnaC"
                     /locus_tag="N7C74_002802"
     CDS             2098..2835
                     /gene="dnaC"
                     /locus_tag="N7C74_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HGU2432636.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2884..3378
                     /gene="yjjA"
                     /locus_tag="N7C74_002803"
     CDS             2884..3378
                     /gene="yjjA"
                     /locus_tag="N7C74_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HGU2432637.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDVENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3632..5923
                     /gene="opgB"
                     /locus_tag="N7C74_002804"
     CDS             3632..5923
                     /gene="opgB"
                     /locus_tag="N7C74_002804"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HGU2432638.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6060..7082)
                     /gene="lgoD"
                     /locus_tag="N7C74_002805"
     CDS             complement(6060..7082)
                     /gene="lgoD"
                     /locus_tag="N7C74_002805"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HGU2432639.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITAGMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7221..8135
                     /gene="lgoR"
                     /locus_tag="N7C74_002806"
     CDS             7221..8135
                     /gene="lgoR"
                     /locus_tag="N7C74_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HGU2432640.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8350..9711
                     /gene="lgoT"
                     /locus_tag="N7C74_002807"
     CDS             8350..9711
                     /gene="lgoT"
                     /locus_tag="N7C74_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2432641.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9760..11424)
                     /gene="tsr"
                     /locus_tag="N7C74_002808"
     CDS             complement(9760..11424)
                     /gene="tsr"
                     /locus_tag="N7C74_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HGU2432642.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            11653..12195
                     /locus_tag="N7C74_002809"
     CDS             11653..12195
                     /locus_tag="N7C74_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432643.1"
                     /translation="MLNRDFDHLSTLLHLFFEREDICQKLNIIDQNNITGWEVWFQVE
                     FANMLCSTDHEWWREQALSCDMRKKPERPTLRTDFLLRKKGWAQDSYIALEIKQNRDA
                     TSCVKNMIADLEKSAKIKRSELDLRSFWTLGITPMIDRERLDSLIDCYLDEKFYLTKS
                     RKKHVLLKEINNTPYCYIVI"
     gene            12488..14638
                     /gene="btsT"
                     /locus_tag="N7C74_002810"
     CDS             12488..14638
                     /gene="btsT"
                     /locus_tag="N7C74_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HGU2432644.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            14688..14891
                     /gene="yjiX"
                     /locus_tag="N7C74_002811"
     CDS             14688..14891
                     /gene="yjiX"
                     /locus_tag="N7C74_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HGU2432645.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            14902..15858
                     /gene="yjiA"
                     /locus_tag="N7C74_002812"
     CDS             14902..15858
                     /gene="yjiA"
                     /locus_tag="N7C74_002812"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HGU2432646.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(15927..>16222)
                     /locus_tag="N7C74_002813"
     CDS             complement(15927..>16222)
                     /locus_tag="N7C74_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HGU2432647.1"
                     /translation="HILPQALVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSEAQH
                     YDFIKKKSIYEKLNSKSSFDLTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         3928 a         4231 c         3963 g         4100 t
ORIGIN      
        1 ctaaaacaat aaattcatta tagtgataat attcatgtga tagtttgttt gcggcgagag
       61 ataattcgct ttttatcacc gagcttgtcc cgataaccag gattaatcag cgcaattttg
      121 atcttaacct accgcaaaac aaccttgctt catgatacaa atggattctc attatctgaa
      181 caatacgcag cacgtctatg acaaagggcg agttatgcaa actgagcaac agcgagccgt
      241 aacacggctt tgtatccagt gtggattatt tcttttgcaa catggtgcgg aaagcgcgtt
      301 ggttgatgag ctttcctcac gactgggtcg ggcgctggga atggacagcg tcgaaagttc
      361 tatctcttcg aacgccatag tgctgacaac tattaaagat gggcaatgcc tgacatcgac
      421 acgtaaaaat cacgatcgcg gcattaatat gcatgtggtg actgaagtcc agcacattgt
      481 gattcttgcg gagcatcatc tgctggatta caaaggcgta gagaaacgat ttagccaaat
      541 tcagccatta cgttacccaa gatggctggt agccttaatg gttggccttt cttgcgcctg
      601 tttctgtaaa ctcaataacg gtggctggga tggtgccgtc atcaccttct ttgccagtac
      661 gaccgcgatg tatatccgcc agctgctggc acaacgtcat cttcatccac agatcaactt
      721 ttgccttacc gctttcgccg ccaccaccat ttccggattg cttttgcaac accccacttt
      781 cagcaatacc cccaccattg cgatggccgc cagcgttctg ctgctagtgc cgggctttcc
      841 gttgattaat gccgtcgccg atatgtttaa aggccacatc aataccggac tggcacgctg
      901 ggcgatcgcc agtctgctga cactggctac ctgcgtcggc gtagtgatgg cactgacgat
      961 ttgggggcta cgcggatggg tgtgatcgaa tttctgttag cgttggcgca ggatatgatc
     1021 ctcgccgcca ttcctgcggt cggctttgcg atggtgttca acgttcccgt gcgggcgtta
     1081 cgctggtgtg cgctgcttgg ctcgataggt catggttccc gaatgatctt gatgaccagc
     1141 gggttgaata ttgagtggtc aacctttatg gcctccatgc tgggcggcac cattggtatt
     1201 caatggtcgc gctggtatct ggcgcatccg aaagtgttta ccgtggcggc cgttatccct
     1261 atgttcccgg gcatatcggc ttataccgca atgatttcgg cggtaaaaat cagccagtta
     1321 ggttacagcg aaccgttgat gattaccctg ttaaccaact ttcttacagc ttcatcgatt
     1381 gttggtgcgt tatccatcgg tctttccatt cctggattat ggttgtaccg caagcgccct
     1441 cgcgtataaa attgcctcgc ttaagcacac ggatgagaga cagcctcctc tcctccgtgt
     1501 gttactataa aagttatctc ccttctcgtt catcgttcca tatttgagaa acagtatgtc
     1561 ttccagagtt ttgaccccgg acgtcgttgg tattgacgcc ctggtacacg atcaccaaac
     1621 cgttctggca aaagctgaag gcggtgtggt tgccgtattt gctaacaatg ccccggcgtt
     1681 ttatgccgtc acgcctgcac gcctggctga actgctggcg ctggaagaaa agctggcgcg
     1741 tccgggaagc gatgtcgatc tggacgatca actctatcag gaaccgcaag ccgctcccgt
     1801 tgctgtaccc atggggaaat tcgccatgta tccggactgg caacccgatg ccgactttat
     1861 ccgcctggcg gctctatggg gcgtggcgct aagagagccg gtaaccaccg aagaactggc
     1921 ctcattcatt gcctactggc aggcggaagg taaagtcttt caccatgtgc agtggcaaca
     1981 aaaactggcg cgcagcctgc aaatcggtcg tgccagcaac ggcggactgc cgaaacgaga
     2041 tgtgaatacg gtcagtgaac ctgacagcca aattccacca ggattcagag ggtaacgatg
     2101 aaaaacgttg gcgacctgat gcaacgcctg caaaaaatga tgcctgccca tatcaaacct
     2161 gcattcaaaa cgggtgaaga gctactggcc tggcagaaag aacaaggggc gatccgctcc
     2221 gccgctctcg aacgtgaaaa tcgggcgatg aaaatgcagc gcacctttaa ccgctccggt
     2281 attcgcccac tgcatcagaa ctgctccttt gagaactatc gcgttgagtg tgaagggcag
     2341 atgaatgcgt taagcaaagc gcgccagtat gtcgaagagt ttgacggcaa catcgccagc
     2401 ttcatctttt ctggtaagcc aggaaccggc aaaaaccatc tggcggcggc aatctgcaac
     2461 gagctgctgc tacgcggtaa atccgtattg atcatcaccg tggccgatat tatgtcggcg
     2521 atgaaagata ccttcaggaa tagcggtacc agcgaagaac aactgcttaa cgatctgagc
     2581 aacgttgatc tgctggtgat cgatgagatc ggcgtgcaga ccgaatcgaa atacgaaaaa
     2641 gtgatcatca accagatcgt cgatcgccgc tcttcttcca aacgcccaac cgggatgctg
     2701 accaacagta atatggaaga gatgaccaag ttgctgggcg aacgcgtgat ggaccgtatg
     2761 cgcctgggta acagtttgtg ggtgatcttc aactgggata gctaccgtag ccgtgtaaca
     2821 ggtaaagagt attaatcccc gtttctggcg gtatactgcg acacaattcc accttgagta
     2881 atgatgatga aaactgttaa acatcttctg tgctgtgcca ttgccgccag cgcattaatt
     2941 tccaccgggg tgcatgctgc gtcctggaaa gatgcgctct ccagcgccgc cagcgaactt
     3001 ggcaaccaaa acagcacgac acaggaaggc ggttggtcgc tcgcgtcatt aactaacttg
     3061 cttagcagcg gaaaccaggc cttaagcgca gataacatga acaatgccgc aggcattctg
     3121 caatactgcg cgaagcaaaa gctggcttcg gtaaccgatg tcgaaaacat caagaaccag
     3181 gtgctggaaa agctgggcct gaacagtgaa gagcaaaaag aagacaccaa ctatctggac
     3241 ggcattcagg gtttgctgaa aaccaaagat ggtcagcaac tcaatctgga taacatcgga
     3301 acaactccgc tggcagaaaa ggtgaaaacc aaagcctgcg atctggtgtt aaagcagggg
     3361 ctgaacttca tttcctgaac cgcgaaacgc cgcgttttag ctgttacccg gctgacgcgg
     3421 ctctctcctg cccttttccc tgctcctgga cggttttccc cctgttacac gggaagcgac
     3481 cgcgatcaaa aaagtctgtt tataaactaa ttcctaacaa catcgcattt ttttgacact
     3541 agaattgcag caatatggcg acgccattac attgtgcttc tcaaaaagct taaacgttgg
     3601 ctaaacggcc cggtttagtg aggatcgccc gttgtcagaa ctactctctt tcgccctttt
     3661 tctcgcctct gtgctgattt acgcatggaa agcgggacgt aacacctggt ggtttgcagc
     3721 cacattaacg gtgctggggc tatttgtcgt tttaaatatc accctgtttg ccagcgacta
     3781 ttttactggc gatggtatta acgacgcggt tctctatacc ttaaccaaca gcctgaccgg
     3841 tgctggcgtc agcaaataca ttttgccggg tatcggcatt gtgctggggc tgacagcggt
     3901 gttcggtgcg ctgggctgga tcctgcgccg tcgtcgccat catccgcacc attttggtta
     3961 cagcctgctg gcgctcttac tggcgctggg ttcggtggac gccagcccgg catttcgtca
     4021 gataacggaa ctggtgaaat cccagtcacg cgacggcgac ccggactttg cggcttatta
     4081 taaagagccg tcgaaaacta tccctgaccc gaaactcaac ctggtttata tctacggcga
     4141 aagtctcgag cggacctatt ttgataacga ggctttcccg gacctcacgc ctgaactggg
     4201 cgcgttgaaa aatgaaggcc tggatttcag ccacacgcag cagctgccgg gaacagatta
     4261 cacgattgcg ggcatggtgg cttctcagtg cggcataccg ctgtttgccc cctttgaagg
     4321 caacgcctcc gcttctgttt ccagcttctt cccgcagaac atctgtctgg gcgatatcct
     4381 gaaaaactcg ggttatcaga actatttcgt gcagggcgcg aatctgcgtt ttgccggtaa
     4441 agatgtgttc ctgaagtcgc atggcttcga ccacttatac ggctcagaag agctgaaaag
     4501 cgtggtagcc gacccgcact atcgcaacga ctggggattc tacgacgata ccgttctcga
     4561 tgaagcgtgg aaaaagtttg aagagctttc ccgctcaggt cagcgattct cactgtttac
     4621 cctgacagtc gatacccatc acccggatgg ttttatctct cgtacctgta accgcaaaaa
     4681 atatgatttt gacggtaaac cgaatcagtc attcagcgcg gtaagttgca gccaggagaa
     4741 tatcgcgacg tttatcaaca aaatcaaagc gtcaccgtgg tttaaagata ctgtcatcgt
     4801 cgtctcttct gaccatttag cgatgaacaa cacggcgtgg aaatacctca ataagcagga
     4861 tcgcaataac ctgttttttg tcattcgtgg cgacaagccg cagcaagaga cgctggcagt
     4921 gaagcgtaac acgatggata acggagcgac agtgctggac attctcggtg gcgataacta
     4981 tctcggactt ggtcgtagca gtttatccgg gcagtcaatg tcggaaatct tcctcaatat
     5041 caaagagaaa acattggcgt ggaagccgga tatcatccgt ctgtggaaat tccctaaaga
     5101 gatgaaagag ttcaccatcg accagcagaa aaacatgatt gccttctcgg gtagccattt
     5161 ccgtttgccg ctgctgttgc gggtttcaga caaacgcgtg gaaccgctgc cagaaagcga
     5221 atactcagca ccgctgcgtt tccagctggc cgatttcgct ccacgcgaca atttcgtctg
     5281 ggttgaccgt tgctacaaga tggcacaact ctgggctccg gaactggcac tttccaccga
     5341 ctggtgtgtc tcgcaggggc aacttggcgg tcagcaaatt gttcagcatg ttgacaaaac
     5401 aacatggaag agcaagacgg catttaaaga tacggtcatc gacatggcgc gttacaaagg
     5461 caatgtcgat acgctgaaga ttgttgataa cgatattcgc tacaaagccg acagtttcat
     5521 ctttaacgtc gccggtgcgc cagaagaggt gaaacagttt agcgggattt cccgtccgga
     5581 gtcgtggggc cgctggtcca acgcgcagct gggcgatgaa gtaaaaatcg agtacaagca
     5641 tccgctgccg aagaaatttg acctggtgat taccgccaaa gcatacggca ataacgccag
     5701 ccgtcctatt ccggtacgcg taggcaatga agaacaaact cttgtgctgg gcaatgaagt
     5761 gaccaccacc acactgcatt tcgataaccc aaccgatgcc gacacactgg taattgtgcc
     5821 gccggaacct gtctcaacca acgaagggaa tatcctcgga cactcgccgc gtaagctcgg
     5881 gatcggcatg gtggaaatta aagtggtaga acgtgaaggg taattcctga ctgatgtgta
     5941 ggtcgcatag ggtgcgacct acacaccttc tccggcttac aacacattat taagaaacaa
     6001 aaaaaacagc gcccaacact tccccggacg ctgcatggca actttaataa tctcgccttt
     6061 cagaaagtaa ttacgccttt aattaactca cgattgttaa tcacatcgcg ctcgtaggtt
     6121 tctgccagcg tggcgaacgg atagcgatgg gttaacatca tgccagcagt gatttttcct
     6181 tccgccatca gtcgaccgac tttagcaaaa tcttccggcg tggcgttgcg gctgcccatc
     6241 atcgtcgttt cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacagg
     6301 ccgacaaata ccaccgtgcc gccgtgacga atcagattca cggtgttatt catcgcatgt
     6361 tgattacctg tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc
     6421 tgcgcgtcaa aatcttcggc tgacgggtcc agcacgggca actccagacg cgttgccaca
     6481 tgttcacggc gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg
     6541 attgccgccg cgcccagacc gattggcccg gccccgacca ccagcacctg ctcgccggga
     6601 gcaatggctg cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc
     6661 gcctgcgggt caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca
     6721 ctaaaaccgc catcctgatg cacgccaatg actgaaattt tttcacagca attggtacgc
     6781 ccgcttttac acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga
     6841 ccatttttaa gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg
     6901 cctaaaaccc gtggataact aaaaaatggt tgatttccac cccaggcatg aatatcggta
     6961 ccgcaaatcc cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc
     7021 tcgcgttgtt tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac
     7081 atgattcggc tctccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg
     7141 gaacggaaaa tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat
     7201 ggactcacaa ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc
     7261 aggtcattga tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc
     7321 tggcggaaat gtacaacatc agccgcacta cagtgcgtca tattctcagc cacttacgcg
     7381 aatgcggcgt cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg
     7441 atgacggttt cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg
     7501 cttttttcac gatgataaac cagcgccagt tacgcccagg ggaaactttt tctgaactgc
     7561 aactggctcg ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg
     7621 gccgttataa tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc
     7681 aatcctacgc tgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc
     7741 actttctcaa cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctcgaac
     7801 gtcatcggct actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca
     7861 gagattttca ctccttactg ctttcagctg ccgacaatat cttttttgat caatcgcttg
     7921 agatcatctc agtcatcttt cattttcact atcaatggga cgaaagcgat cttaaacagc
     7981 gcaatatcat tgccgtcgat gagcatatga ccattctcag cgctctgatt tgccgcagtg
     8041 accttgatgc cacccttgcg ctccgtaacc atttgaattc agcgaaacaa tcaatgattc
     8101 gatccattaa tgagaacacg cgttatgctc attaagtacg gattaaaaac agcaaactat
     8161 tagcgggtgc acggttttat caaaagataa ataccgggaa attcccgctt acctatgctc
     8221 acaatcagtc gactaaaaat gtttttgtga gcggtagtaa agtcctaaaa ctttaacctg
     8281 ccgttaatgt caaccttgcg taatgcacaa ttacgtaaac aattaaaatt ataaatacag
     8341 gagtccggcg tggaaaaaga aaatatcacc ctcgatccgc gttcttcatt tactccatct
     8401 tcgtcggcag atattcccgt gccaccagat ggattagttc aacgcagtac ccgaattaaa
     8461 cgcattcaaa ccaccgccat gttgttatta ttttttgcgg cggtaatcaa ttatctcgac
     8521 cgcagttcgc tgtcggtagc aaatttaacg attcgtgaag aattgggatt aagtgccacc
     8581 gaaatcggcg ctttgctctc cgtgttttca ctcgcttacg gcattgcgca acttccttgc
     8641 ggcccactat tggatcgtaa aggcccacgc ctgatgctgg gactggggat gttcttctgg
     8701 tcactgttcc aggccatgtc tggcatggtg cacagcttta cgcagttcgt gttggtgcgt
     8761 atcggtatgg ggattggtga agcgccgatg aacccatgcg gtgtaaaagt cattaacgac
     8821 tggttcaaca tcaaagagcg cggacgcccg atgggcttct tcaacgcagc ttctaccatt
     8881 ggcgttgccg taagcccacc gattctggcg gcgatgatgc tggtgatggg ctggcgcggg
     8941 atgtttatta ccattggtgt actggggatt tttctcgcca tcggctggta tatgctctat
     9001 cgcaaccgcg agcacgtaga actgactgcc gttgaacaag cttatctcaa tgcaggtagc
     9061 gtcaatgccc gccgagatcc gctcagtttt gccgaatggc gcagcctgtt ccgtaaccgc
     9121 acaatgtggg gaatgatgct cggattcagt ggcatcaact acactgcgtg gctgtatctg
     9181 gcctggcttc ctggttacct gcaaacagcc tataacctgg atttaaaaag cacagggttg
     9241 atggcggcta tccctttcct gtttggtgct gccgggatgc tggtcaacgg ttacgttact
     9301 gactggctgg tcaaaggggg aatggctccg attaaaagcc gtaagatctg cattattgcc
     9361 gggatgttct gttctgccgc ctttacgctg atagtaccac aagcgacaac atccatgatg
     9421 gcggttctgc tgattggcat ggcactgttc tgtattcact ttgccggaac atcctgctgg
     9481 ggcttgatcc acgtcgcagt tgcttctcgc atgactgcgt cggtgggcag tatccagaac
     9541 tttgccagct tcatctgcgc ctctttcgcg ccgatcatta ctggttttat tgttgataca
     9601 acccactcat tccgtctggc actaatcatt tgcggttgcg tcaccgcagc gggagcactg
     9661 gcgtacatct tcctggttcg tcagccgatc aacgacccgc gtaaagatta aactccctat
     9721 gcccgataac attttgctta tcgggcattt ttatggcgat taaaacgttt cccagttctc
     9781 cccgctatct gccactgcca ttttacgcgg cgtagctggc gtcacggttt ttaccacagc
     9841 cgatgtttca cgctgctgtt gctgctgttg ctgaatccgg aacactgcca cagcttcggt
     9901 caggcgactg gcctgctctt ccaacgcggc ggcggcagcg gcagactctt ccaccagcgc
     9961 agcgttctgt tgagttaccc ggtccatctc agcaaccgct aagccaacct gatcgatacc
    10021 acggctctgc tcatcagaag cagaagctat ttcgcccata atgtccgtca cgcgggtcac
    10081 agcactgaca atctccgcca ttgtttcccc ggcgctttcg accagcgtag agccaacatc
    10141 cactttcccc accgagtctt caatcaggct tttaatttca cgagccgcct gggcgctacg
    10201 ctgggccaga ttacgcactt ctcccgcaac caccgcaaaa ccacgtcctt gctcacccgc
    10261 acgcgctgct tcaaccgccg cgttcaaagc cagaatattg gtctggaagg caatgccgtc
    10321 aattacgctg ataatatcgg cgattttctg cgaactggtg gagatatcac gcatagtctg
    10381 caccacgtta tctaccactt tgccgccgcg ttgcgccgtt tcagaagcac ttaacgccag
    10441 gtggctggcc tggcgcgcat tctcggcgtt ctgtttcacc gttgcggtga gttgctccat
    10501 gctggctgcc gtctcttcca gcgaagcggc ctgttgctcg gtgcgcgaag agagatcgtt
    10561 attgccggta gcgatttcgc tggcaccgct atagatggca ttggccccgt tgcgcacatc
    10621 accgacggta cgcatcagct ctccctgcat atggcgcaaa ctctctgcca gttgccccat
    10681 ctcattagag ccatccacct caatcggttt caccagatcg ccgcctgcaa tatgacgaat
    10741 gctgtcaatc aggcgattca ttggcgctac cagcgaggct ttaataccga accagacggc
    10801 gaagatgacc gccagtacga cgatcatcac gcccaccaga atccacatcg cctggctgta
    10861 ggaggcattg ttatcgctga cggcgatatc atagagccga tcgttttgct ccatgtaagc
    10921 cacatactgc ttctcgaaac cgtcctgata tccctgggtc ggctgatcaa agaactcgtt
    10981 gattttgcct gcacctaaca gttggatcag ctccgccagc gcattgtgat aaatatcgta
    11041 attacgtttg atctctgccg ctgcggcggt gctctgacgc gggtcacgcg gcaacgcttc
    11101 gtaatctgcc cagttttttt ccgcctgttt caacgaaata ctggcactct gcatcagctc
    11161 agcaacggtt gaaccgctac caatattatt ctgatccatc atgtagcgga tacccgcgcg
    11221 gttgagggtg ttacgcgtct gcaacaacgc gacccagctg ccattcagcg tggattgctg
    11281 ctggcgaatg gtttgtaaaa cagtgaaatt ttctttgtca ttctttaagg cattaaagaa
    11341 cagaccgcct gatgtcagtt gtaaaaggcc aaaaacggcc aaaaccagca gtaagctggt
    11401 cacgatcttg atacgtttta acatggtttc tctttcctgt ggaccgatgc aaaattttcg
    11461 gcctgaaatt agaaaacttt ataggtagcg acttgaaact ggttaatttt acctcacttt
    11521 ttttaatttt tgttcaaaaa tacaataacc acttattgat acattttctt ttcttatttt
    11581 tttcacacga attgtatcta ttttgaacga aatattttat agagtgtgga attttaagaa
    11641 aggacatctg aaatgttaaa ccgtgacttc gatcatctct caacgctatt acatctcttt
    11701 tttgaacgtg aggatatttg ccaaaagctt aatattattg accaaaacaa tatcaccggt
    11761 tgggaagtgt ggttccaggt agagtttgct aatatgctct gctcaacaga ccatgaatgg
    11821 tggcgtgaac aagcactttc ctgtgatatg cgcaaaaaac ctgagcgccc gacactacgc
    11881 acagatttct tactacgtaa aaaaggctgg gcacaagata gctatatcgc gcttgaaata
    11941 aaacaaaaca gagacgcgac cagttgtgtt aaaaatatga tcgcagactt agaaaaaagc
    12001 gcaaaaataa aacgttccga actcgattta cgcagtttct ggaccttggg tatcacccct
    12061 atgatagaca gggaaaggct ggactctctc atcgattgct atttagatga gaaattttat
    12121 cttactaaat ctcgtaaaaa acacgtttta cttaaagaaa taaataacac accatattgc
    12181 tatattgtta tttaatattt atagtatgaa tataattttt aaatgagtta cacaaccgag
    12241 ggctaaaaat cgcttcggtt gtgatcatca tcacataaat aaccttcata aaccttgctc
    12301 aaaaccgctc aaccgcaaaa ctgaccgctt acgtccctaa aataaccact cagttattta
    12361 ccttacttta cgcgcgcgta actctggcag catcattgca ggatagcggt caatttacct
    12421 cctcaaacgc aacgcaaacc tagaacggct tcggccaact attaatcaat acatgccagg
    12481 ttttactatg gatacgaaaa aactattcaa gcacataccc tgggtgattc tcggaatcat
    12541 cggtgcattc tgcctcgcgg tagttgcatt acgtcggggg gagcacgtca gcgccctgtg
    12601 gatcgtggtc gcctctgtgt cggtgtatct ggtggcgtat cgctactaca gtctgtacat
    12661 cgcccagaag gtgatgaaac tcgaccccac gcgcgcgacg cctgcggtta ttaacaacga
    12721 cggtctgaac tacgttccga ccaaccgtta cgtgttgttt ggtcaccact tcgccgctat
    12781 cgccggtgct ggtccgctag tgggaccggt tctcgccgcg cagatgggct acctgcctgg
    12841 cacgctgtgg ctgctggcgg gggtagtgct ggccggtgcg gttcaggact ttatggtgct
    12901 gtttatctcc tctcgccgta acggcgcatc tctcggtgag atgatcaaag aagagatggg
    12961 gccagtaccg gggactatcg cgctgtttgg ctgtttctta atcatgatca tcatcctcgc
    13021 cgtcctggcg ctgattgtgg ttaaagccct ggcagaaagt ccgtggggtg tcttcaccgt
    13081 ttgctcaacc gtaccaattg cgctgtttat gggtatctac atgcgcttta tccgtccggg
    13141 gcgtgtgggt gaagtctctg ttattggtat cgtgctgctg gttgcctcta tctacttcgg
    13201 tggcgtgatt gctcacgatc cgtactgggg tccggcactg acctttaaag acaccaccat
    13261 taccttcgcg ctgattggct atgcgtttgt ttccgcgctg ctgccggtgt ggctgatcct
    13321 cgcaccacgc gactatctgg caaccttcct gaagatcggc gttatcgtcg gcctggcgct
    13381 gggtatcgtg gtgctgaacc cggaactgaa aatgcctgcg atgacccagt acattgacgg
    13441 tactggcccg ctgtggaaag gcgctctgtt cccgttcctg ttcatcacca tcgcctgtgg
    13501 tgcggtatct ggcttccacg cgctgatctc ttccggtacg acgccgaaac tgctggctaa
    13561 cgaaaccgac gcgcgtttca tcggctacgg cgcaatgctg atggagtcct tcgtggcgat
    13621 tatggcgctg gttgctgcat ccatcatcga accgggtctt tacttcgcga tgaacacccc
    13681 gcccgcgggc cttggcatca ccatgcctaa cctgcatgaa atgggtggcg agaacgcgcc
    13741 gatcatcatg gcgcagctga aagacgttac cgcacacgcg gcagcgaccg tcagctcttg
    13801 gggcttcgtg atttcgccag agcagatcct gcaaaccgcg aaagacattg gtgagccttc
    13861 tgtcctgaac cgtgcaggtg gtgcgccgac gctggcggta ggtatcgctc atgtgttcca
    13921 caaagtgctg ccgatggctg acatgggctt ctggtatcac ttcggtattc tgttcgaagc
    13981 cctgttcatc ctgaccgcgc tggatgcggg tacccgttct ggccgcttta tgctgcaaga
    14041 cctgctgggt aacttcatcc cgttcctgaa aaaaaccgac tctctggttg ctggtatcat
    14101 cggtactgcg ggctgtgtgg gtctgtgggg ctacctgctg tatcagggcg tggtcgatcc
    14161 gctgggcggc gttaagagcc tgtggccgct gttcggtatc tccaaccaga tgctggcagc
    14221 cgtagcgctg gtactgggca ccgttgtgct gattaagatg aagcgcaccc aatacatctg
    14281 ggtaactgtt gttccggctg tatggctgct tatctgcacc acctgggcgc tgggtctgaa
    14341 actgttcagc accaacccgc agatggaagg cttcttctac atggcaagcc agtacaaaga
    14401 gaagattgct aacggtactg accttacggc gcagcagatt gccaacatga accacatcgt
    14461 tgtgaacaac tacaccaacg caggcctgag tattctgttc ctgattgtgg tgtacagcat
    14521 catcttctac ggtttcaaaa cctggcttgc ggtacgtaac agcgacaaac gtactgacaa
    14581 agaaacaccg tacgttccaa tcccggaagg cggcgtgaag atctcttcgc accactaacc
    14641 gtgtttagcc ccgcttcggc ggggctttgt tcttatcaga gtgaactatg tttggtaact
    14701 taggacaggc aaaaaaatat ctcggccagg cggcgaagat gttgattggc attccagact
    14761 acgacaacta cgtcgagcat atgaagacca accatcccga caagccgtac atgagctatg
    14821 aagaattctt ccgcgaacgc cagaacgcgc gctacggcgg cgatggtaaa ggcggtatgc
    14881 gctgttgtta atggagagag catgaacccg attgcagtta ccctactcac cggtttttta
    14941 ggcgcgggca aaaccaccct gctgcgccat attcttaacg aacaacacgg ctacaagatt
    15001 gccgtgattg aaaacgaatt tggcgaagtc tccgttgatg atcaactgat tggcgaccgc
    15061 gccacgcaga tcaaaacgct gaccaacggc tgcatctgct gttcgcgctc caacgagctg
    15121 gaagacgcac tactcgacct gctggataat ctcgataagg gcaatattca gttcgatcgt
    15181 ctggtcattg aatgcaccgg catggccgat cccggcccga ttattcagac ctttttctcc
    15241 catgagattt tatgccagcg ttacctgctg gacggcgtga ttgcgctggt ggatgcggta
    15301 catgccgatg agcagatgaa ccagttcacc atcgcccagt cccaggttgg ctacgccgac
    15361 cgtattctgc tgaccaaaac cgacgtcgca ggcgaagcag aaaaactgcg cgaacgcctg
    15421 gcgcgcatca acgcccgtgc accggtctac accgttaccc acggcgacat cgacctgggc
    15481 ctgctgttca acaccaacgg ttttatgctg gaagaaaacg tcgtcagcac caaaccgcgt
    15541 ttccacttta tcgcggataa acaaaacgat atttcgtcga ttgtggtaga actggattac
    15601 ccggtagata tcagcgaagt ttcccgcgtg atggaaaacc tgctgctgga atcggcggat
    15661 aaactgctgc gttacaaagg gatgctgtgg attgacggcg aacctaaccg cctgctgttc
    15721 cagggcgtcc agcgcctcta cagcgccgac tgggaccgcc cgtggggcga tgaaaaaccg
    15781 catagcacaa tggtttttat cgggattcag ttgccggagg aagagattcg ggcagcgttt
    15841 gcggggttga ggaagtaagt tcgggaaaga tcaacttttt gagagttcca ccgcaacatt
    15901 attacgagtg gaactcttta gatatgtcag accaaccaaa gcttcttcaa ttgcgttttc
    15961 attttttcat gtctcatttt cagttcagcc aatccccatt ctgaagaatt acaaacatct
    16021 tttgttaaat caaaagagct tttactatta agtttctcat aaatagattt ctttttgata
    16081 aaatcatagt gttgtgcttc agaatttttg gaaccactta tgagtgttaa attacctatt
    16141 ttatgcagcc agtagacatg ctcttgaggt tggaatctat taatccagta ttcattaact
    16201 aatgcttgag gaagaatatg ct
//
LOCUS       DBFIFM010000104        16218 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.104, whole genome shotgun sequence.
ACCESSION   DBFIFM010000104 DBFIFM010000000
VERSION     DBFIFM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.104"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..394)
                     /locus_tag="N7C74_002814"
     CDS             complement(<1..394)
                     /locus_tag="N7C74_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432648.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSDIPTPSSEPINQFAPQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENIFITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQEQLTAE"
     gene            917..2845
                     /gene="thrS"
                     /locus_tag="N7C74_002815"
     CDS             917..2845
                     /gene="thrS"
                     /locus_tag="N7C74_002815"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HGU2432649.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKIAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2849..3391
                     /gene="infC"
                     /locus_tag="N7C74_002816"
     CDS             2849..3391
                     /gene="infC"
                     /locus_tag="N7C74_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HGU2432650.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3488..3685
                     /gene="rpmI"
                     /locus_tag="N7C74_002817"
     CDS             3488..3685
                     /gene="rpmI"
                     /locus_tag="N7C74_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HGU2432651.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3738..4094
                     /gene="rplT"
                     /locus_tag="N7C74_002818"
     CDS             3738..4094
                     /gene="rplT"
                     /locus_tag="N7C74_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HGU2432652.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4211..4335
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4217..4261
                     /gene="pheM"
                     /locus_tag="N7C74_002819"
     CDS             4217..4261
                     /gene="pheM"
                     /locus_tag="N7C74_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HGU2432653.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4544..5527
                     /gene="pheS"
                     /locus_tag="N7C74_002820"
     CDS             4544..5527
                     /gene="pheS"
                     /locus_tag="N7C74_002820"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HGU2432654.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5542..7929
                     /gene="pheT"
                     /locus_tag="N7C74_002821"
     CDS             5542..7929
                     /gene="pheT"
                     /locus_tag="N7C74_002821"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HGU2432655.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQYKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7934..8233
                     /gene="ihfA"
                     /locus_tag="N7C74_002822"
     CDS             7934..8233
                     /gene="ihfA"
                     /locus_tag="N7C74_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HGU2432656.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8334..9314
                     /gene="btuC"
                     /locus_tag="N7C74_002823"
     CDS             8334..9314
                     /gene="btuC"
                     /locus_tag="N7C74_002823"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HGU2432657.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPMLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRALL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9377..9928
                     /gene="btuE"
                     /locus_tag="N7C74_002824"
     CDS             9377..9928
                     /gene="btuE"
                     /locus_tag="N7C74_002824"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HGU2432658.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLISAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9928..10677
                     /gene="btuD"
                     /locus_tag="N7C74_002825"
     CDS             9928..10677
                     /gene="btuD"
                     /locus_tag="N7C74_002825"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HGU2432659.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     DVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10755..11219
                     /gene="nlpC"
                     /locus_tag="N7C74_002826"
     CDS             10755..11219
                     /gene="nlpC"
                     /locus_tag="N7C74_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HGU2432660.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11466..12179
                     /gene="ydiV"
                     /locus_tag="N7C74_002827"
     CDS             11466..12179
                     /gene="ydiV"
                     /locus_tag="N7C74_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HGU2432661.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12242..13678
                     /gene="selO"
                     /locus_tag="N7C74_002828"
     CDS             12242..13678
                     /gene="selO"
                     /locus_tag="N7C74_002828"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HGU2432662.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPDKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLNDYEPGFICNHSD
                     HQGRYSFDNQPAVALWILQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13682..13873)
                     /gene="hemP"
                     /locus_tag="N7C74_002829"
     CDS             complement(13682..13873)
                     /gene="hemP"
                     /locus_tag="N7C74_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HGU2432663.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(14005..15051)
                     /gene="aroH"
                     /locus_tag="N7C74_002830"
     CDS             complement(14005..15051)
                     /gene="aroH"
                     /locus_tag="N7C74_002830"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HGU2432664.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRINHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(15208..16041)
                     /gene="ppsR"
                     /locus_tag="N7C74_002831"
     CDS             complement(15208..16041)
                     /gene="ppsR"
                     /locus_tag="N7C74_002831"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HGU2432665.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT         4072 a         3767 c         4296 g         4083 t
ORIGIN      
        1 tttcggcagt taactgttcc tgttcaatat aatccagtat gcttcgcaaa tggttagctt
       61 taataggcgt ggtttccagt aataaggaaa tgatttcttt tttatagtgt aatgtaatga
      121 atatattttc tgcatggatg ggtgaagtgg caaagttaat ggtaaaaagg gcagtggaat
      181 aacaattttg tgatattttt cgacagtttg cggtaaattc ctgagtaaca cagtatgaca
      241 gcatcgaact taagtcagag gtatcaagta gggtgatgag ttggggcgca aattgattaa
      301 taggctctga tgaaggcgtt ggtatgtcgc tatcgatgtg cattctatga ggagacttat
      361 aatgagttct gataataatg tcgttttgac tcataatcta ttcgccttat atatgtctgg
      421 aggttttgag tatgatgatt aattattatc gccagaacga aatgtgcacg aaaaattgct
      481 ataagaattg tgtcactttt atcattgtga tatgaccact tcgtcattaa tcttatgatc
      541 gtttaaatag taccgccaaa tcacgcaacg aactttgctg gattcagttt atgctgctgt
      601 aaattcgctc gcagtaaacc tttcagacgc tccgtgatgt tatcagttgt tctttaagcg
      661 ttttgctggt gtactcacta caaacgaatt gcgaatcgaa tcaatgtgaa acggaaaggt
      721 acaatctccc tttttttagt gttaacatcg ctcaaccggg tggaaggtga gaggttaatg
      781 tctttcattt gtgtacctga ttgcgaacca atttagcatt tgttggctag atggtttcgc
      841 aatgaactgt taataaacaa atttttcttt gtatgtgatc tttcgtgtgg gtcaccactg
      901 caaataagga tataaaatgc ctgttataac tcttcctgat ggcagtcaac gccattacga
      961 ccacgctgta agccccatgg atgttgcgct ggacattggt ccaggtctgg cgaaagcctg
     1021 tatcgcaggg cgcgttaatg gcgaactggt tgatgcttgc gatctgattg aaaacgacgc
     1081 acaactgtcg atcattaccg ccaaagacga agaaggtctg gagatcattc gtcactcctg
     1141 tgcgcacctg ttagggcacg cgattaaaca actttggccg cataccaaaa tggcaatcgg
     1201 cccggttatt gacaacggtt tttattacga cgttgatctt gaccgcacgt taacccagga
     1261 agatgtcgaa gcactcgaga agcggatgca tgagcttgct gagaaaaact acgacgtcat
     1321 taagaagaaa gtcagctggc acgaagcgcg tgaaactttc gccaaccgtg gggagagcta
     1381 caaagtctcc attcttgacg aaaacatcgc ccatgatgac aagccaggtc tgtacttcca
     1441 tgaagaatat gtcgatatgt gccgcggtcc gcacgtaccg aacatgcgtt tctgccatca
     1501 tttcaaacta atgaaaacgg caggggctta ctggcgtggc gacagcaaca acaaaatgtt
     1561 gcaacgtatt tacggtacgg cgtgggcaga caaaaaagcg cttaacgctt acctgcagcg
     1621 cctggaagaa gccgcgaaac gcgaccaccg taaaatcggt aaacagctcg acctgtacca
     1681 tatgcaggaa gaagcgccgg gtatggtatt ctggcacaac gacggctgga ccatcttccg
     1741 tgaactggaa gtgtttgttc gttctaaact gaaagagtac cagtatcagg aagttaaagg
     1801 tccgttcatg atggaccgtg tcctgtggga aaaaaccggt cactgggaca actacaaaga
     1861 tgcaatgttc accacgtctt ctgagaaccg tgaatactgc attaagccga tgaactgccc
     1921 gggtcacgta caaattttca accaggggct gaagtcttat cgcgatctgc cgctgcgtat
     1981 ggccgagttt ggtagctgcc accgtaacga gccgtcaggt tctctgcatg gcctgatgcg
     2041 cgtgcgtgga ttcacccagg atgacgcgca tatcttctgt actgaagaac aaattcgcga
     2101 tgaagttaac ggatgtatcc gtttagtcta tgatatgtac agcacttttg gcttcgagaa
     2161 gatcgtcgtc aaactctcca ctcgtcctga aaagcgtatt ggcagcgacg aaatgtggga
     2221 tcgtgctgag gcggacctgg cggttgcgct ggaagaaaac aacatcccgt ttgaatatca
     2281 actgggtgaa ggcgctttct acggtccgaa aattgaattt accctgtatg actgcctcga
     2341 tcgtgcatgg cagtgcggta cagtacagct ggacttctcc ttgccgtctc gtctgagcgc
     2401 ctcctatgtg ggcgaagaca acgagcgtaa ggtaccggta atgattcacc gcgcaattct
     2461 ggggtcgatg gaacgtttca tcggtatcct gaccgaagaa ttcgctggtt tcttcccgac
     2521 ctggcttgcg ccagttcagg ttgttatcat gaatattacc gattcacagt ctgaatacgt
     2581 taacgaattg acgcaaaaac tatcaaatgc gggcattcgt gttaaagcag acttgagaaa
     2641 tgagaagatt ggctttaaaa tccgcgagca cactttgcgt cgcgtcccgt atatgctggt
     2701 ctgtggtgat aaagaggtgg aatcaggcaa aattgccgtt cgcacccgcc gtggtaaaga
     2761 cctgggaagc atggacgtaa atgaagtgat cgagaagctg caacaagaga ttcgcagccg
     2821 cagtcttaaa caattggagg aataaggtat taaaggcgga aaacgagttc aaacggcgcg
     2881 ccctaaccgt atcaatggcg aaattcgcgc ccaggaagtt cgcttaacag gtctggaagg
     2941 cgagcagctt ggtattgtga gtctgagaga agctctggag aaagcagaag aagccggagt
     3001 agacttagtc gagatcagcc ctaacgccga gccgccggtt tgtcgtataa tggattacgg
     3061 caaattcctc tatgaaaaga gcaagtcttc taaggaacag aagaaaaagc aaaaagttat
     3121 ccaggttaag gaaattaaat tccgtcctgg tacagatgaa ggcgactatc aggtaaaact
     3181 ccgcagcctg attcgctttc tcgaagaggg tgataaagcc aaaatcacgc tgcgtttccg
     3241 cggtcgtgag atggcgcacc agcaaatcgg tatggaagtg cttaatcgcg tgaaagacga
     3301 tttgcaagaa ctggcagtgg tcgaatcctt cccaacgaag atcgaaggcc gccagatgat
     3361 catggtgctc gctcctaaga agaaacagta aggccttcaa gtagcactgt ctgtggagcc
     3421 ttcgggcttc acaggctttg ttcgcctttg tttcgtttat taacaatgcg aagtggaagt
     3481 tattaaaatg ccaaaaatta agaccgtacg cggtgctgct aagcgcttca aaaaaaccgg
     3541 taaaggtggt tttaagcaca agcacgctaa cctgcgtcac attctgacca aaaaagcgac
     3601 caaacgtaaa cgtcacctgc gtccgaaagc catggtttcc aaaggcgatc tgggcctggt
     3661 aatcgcgtgc ctgccgtacg cataagccgt taacgttttt aactttttaa ttagaatata
     3721 gatacaggag agcacatatg gctcgcgtaa aacgtggtgt tattgcacgc gcacgtcaca
     3781 agaaaatttt gaaacaagct aaaggctact acggtgcgcg ttctcgcgta taccgcgttg
     3841 ccttccaggc tgttatcaaa gctggtcagt atgcttaccg tgaccgtcgt caacgtaagc
     3901 gtcagttccg tcaactgtgg attgcgcgta tcaacgcagc agcacgtcag aacggtattt
     3961 cttacagcaa attcatcaat ggcctgaaaa aagcctctgt tgaaatcgac cgtaagatcc
     4021 tggctgatat cgcagtattc gacaaagtag cgttcaccgc tctggttgaa aaagcgaaag
     4081 cagctctggc ataagccagt tgaaagaggg agctagtctc cctcttttcg tttcaacgcc
     4141 atcaaaacat tgacttttat cgccgtagcc ttttcaataa aggtcttttg aagagtacca
     4201 aaaggtaacg caagcaatga atgctgctat tttccgcttc tttttttact ttagcacctg
     4261 aatccaggag gctagcgcgt gagaagagaa acggaaaaca gcgcctgaaa gcctcccagt
     4321 ggaggctttt tttgtatgcg cgttttgaaa aataaaaagc cagtctatca ggcgtaattg
     4381 tcgcagccag tctggatacg gccagtgcgg agaaaccgga gcgtacttaa gtacgtgagg
     4441 atttcgagca ctgcccggga ccaaaatggc aagtaaaata gcctgatggg ataggctcta
     4501 agtccaacga accagtgtca ccactgacac aatgaggaaa accatgtcac atctcgcaga
     4561 actggttgcc agtgcgaagg cggccattag ccaggcgtca gatgttgccg cgttagacaa
     4621 tgtgcgcgtc gaatatttgg gtaaaaaagg gcacttaacc cttcagatga cgaccctgcg
     4681 tgagctgccg ccagaagagc gtccggcagc cggtgcggtt atcaacgaag cgaaagagca
     4741 ggttcagcag gcgctgaatg cgcgtaaagc ggaactggaa agcgctgcac tgaatgcgcg
     4801 tctggcggcg gaaacgattg atgtctctct gccaggtcgt cgcattgaaa acggcggtct
     4861 gcatccggtt acccgtacca tcgaccgtat cgaaagtttc ttcggtgagc ttggctttac
     4921 cgtggcaacc gggccggaaa tcgaagacga ttatcataac ttcgatgctc tgaacattcc
     4981 tggtcaccac ccggcgcgcg ctgaccacga cactttctgg tttgacgcta cccgcctgct
     5041 gcgtacccag acctctggcg tacagatccg caccatgaaa gcccagcagc caccgattcg
     5101 tatcatcgcg cctggccgtg tttatcgtaa cgactacgac cagactcaca cgccgatgtt
     5161 ccatcagatg gaaggtctga ttgttgatac caacatcagc tttaccaacc tgaaaggcac
     5221 gctgcacgac ttcctgcgta acttctttga ggaagatttg cagattcgct tccgtccttc
     5281 ctacttcccg tttaccgaac cttctgcaga agtggatgtc atgggtaaaa acggtaaatg
     5341 gctggaagta ctgggctgcg ggatggtgca tccgaacgtg ctgcgtaacg ttggcatcga
     5401 cccggaagtt tactctggtt tcgccttcgg gatggggatg gagcgtctga ctatgttgcg
     5461 ttacggcgtc accgacctgc gttcattctt cgaaaacgat ctgcgtttcc tcaaacagtt
     5521 taaataaggc aggaatagat tatgaaattc agtgaactgt ggttacgcga atgggtgaac
     5581 ccggcgattg atagcgatgc gctggcgaat caaatcacta tggcgggcct ggaagttgac
     5641 ggtgtagaac cggttgccgg tagcttccac ggcgtggtcg ttggtgaagt ggttgagtgt
     5701 gcgcagcatc cgaacgctga caaactgcgt gtgacaaaag tgaatgtcgg cggcgatcgc
     5761 ctgctggaca tcgtctgcgg tgcgccaaac tgccgtcagg gcctgcgtgt ggcggtagcg
     5821 accattggtg ctgttctgcc gggtgatttc aaaattaaag cggcgaaact gcgcggcgaa
     5881 ccgtctgaag ggatgctgtg ctccttttct gagctgggaa tttctgacga ccataacggc
     5941 attatcgaac tgcctgcaga tgcgccgatt ggcactgaca tccgcgaata cctgaaactc
     6001 gatgacaaca ccatcgaaat cagcgtaacg ccaaaccgtg ccgactgttt aggtatcatt
     6061 ggtgttgcgc gtgacgttgc cgtgctgaat cagctgccgc tggttgaacc ggaaatcgtt
     6121 ccggttggtg cgaccatcga cgacacgctg ccgattacag tcgaagcgcc ggaagcctgc
     6181 ccgcgttatc ttggccgtgt ggtaaaaggc attaacgtta aagcgccaac tccgctgtgg
     6241 atgaaagaaa aactgcgtcg ttgcgggatc cgttctatcg atgcagttgt tgacgtcacc
     6301 aactatgtgc tgctcgaatt gggccagccg atgcacgctt tcgataaaga tcgcattgaa
     6361 ggcggcattg tggtgcggat ggcgaaagag ggcgaaacgc tggtgctgct cgacggtact
     6421 gaagcgaagc tgaatgctga cactctggtc atcgccgacc acaacaaggc gctggcgatg
     6481 ggcggcatct tcggtggcga acactctggc gtgaatgacg aaacacaaaa cgtgctgctg
     6541 gaatgcgctt tctttagccc gctgtctatc accggtcgtg ctcgtcgtca tggcctgcat
     6601 actgatgcgt ctcaccgtta tgagcgtggc gttgatccgg cactgcagta caaagcgatg
     6661 gaacgtgcga cccgtctgct gattgacatc tgcggtggtg aggctggtcc ggtaattgat
     6721 atcaccaacg aagcaacgct gccgaagcgt gcaaccatca ctttacgtcg tagcaaactg
     6781 gatcgcctga tcggccatca tattgcggat gagcaggtaa ctgacattct gcgtcgtctc
     6841 ggctgcgaag tgaccgaagg caaagacgag tggcaggcag ttgcgccgag ctggcgtttc
     6901 gacatggaga ttgaagaaga tctggtcgaa gaagtcgcgc gtgtttacgg ctacaacaac
     6961 atcccggatg agccggtaca ggcaagcctg attatgggta ctcaccgtga agctgacctg
     7021 tcgctcaagc gcgtgaaaac gctgctcaac gataaaggct atcaggaagt gatcacctat
     7081 agcttcgttg atccgaaagt gcagcagatg atccatccag gcgttgaagc cttactgctg
     7141 ccaagcccga tctctgttga aatgtcagca atgcgtcttt ctctgtggac cggcctgctg
     7201 gcaaccgtgg tgtacaacca gaaccgtcag cagaaccgtg tgcgcatttt cgaaagcggt
     7261 ctgcgtttcg taccagatac tcaggcaccg ttgggcattc gtcaggatct gatgttagcc
     7321 ggtgtgattt gcggtaaccg ttacgaagag cactggaacc tggcaaaaga gaccgttgat
     7381 ttctatgatt tgaaaggcga tcttgaatcc gttctcgacc tgaccggtaa actgaatgag
     7441 gttgagttcc gtgcagaagc gaatccggca ctgcatccgg ggcaatccgc agcgatttat
     7501 ctgaaaggtg aacgtattgg ttttgttggg gttgttcatc ctgaactgga acgtaaactg
     7561 gatcttaacg gtcgcactct ggtgttcgaa ctggagtgga acaagctcgc agaccgcgtg
     7621 gtgcctcagg cgcgcgagat ttctcgcttc ccggcgaacc gtcgtgacat cgcggtggtg
     7681 gtcgcagaaa acgttcccgc agcggatatt ttatccgaat gtaagaaagt tggcgtaaat
     7741 caggtagttg gcgtaaactt atttgacgtg taccgcggta agggtgttgc ggaggggtat
     7801 aagagcctcg ccataagcct gatcctgcaa gataccagcc gtacactcga agaagaggag
     7861 attgccgcta ccgtcgccaa atgtgtagag gcattaaaag agcgattcca ggcatcattg
     7921 agggattgaa cctatggcgc ttacaaaagc tgaaatgtca gaatatctgt ttgataagct
     7981 tgggcttagc aagcgggatg ccaaagaact ggttgaactg tttttcgaag agatccgtcg
     8041 cgctctggaa aacggcgaac aggtgaaact ctctggtttt ggtaacttcg atctgcgtga
     8101 taagaatcaa cgcccgggac gtaacccgaa aacgggcgag gatattccca ttacagcacg
     8161 gcgcgtggtg accttcagac ccgggcagaa gttaaaaagc cgggtcgaaa acgcttcgcc
     8221 caaagacgag taatctgatc taactaaaaa ggccgctctg cggccttttt tcttttcact
     8281 gtcgaagagt caccgtaaaa tcaacgccat gacacttcag cagaacggat accatgctga
     8341 cacttgcccg ccaacaacag cgacaaaata ttcgctggtt attatgcctg tcagttttga
     8401 tgctgctggc gcttctctta agcctttgcg ccggtgaaca atggatctcg ccaggtgact
     8461 ggttttctcc tcgtggcgaa ctgttcgtct ggcaaattcg cctgccacgt acgctggctg
     8521 tattgctggt tggtgcggcg ctggctatat ccggcgctgt aatgcaggcg ttgtttgaaa
     8581 atcctctggc agaacctgga ctacttggcg tctctaacgg cgcaggcgtg gggcttatcg
     8641 ccgcggtatt gcttgggcaa gggcaactcc ccaactgggc gctagggctg tgtgcgattg
     8701 ctggggcgct tatcatcact ttaatactct tacgtttcgc ccgtcgtcat ctttcgacca
     8761 gtcggttatt gctggctggc gttgcattag ggattatctg tagcgcacta atgacgtggg
     8821 ctatctactt ttccacctct gttgatttac gtcagctgat gtactggatg atgggcggtt
     8881 ttggcggcgt agactggcgg caaagctggc tgatgctggc attgatcccc atgttgttgt
     8941 ggatctgttg tcagtccagg ccgatgaata tgttagcact tggcgagatc tcggcgcggc
     9001 aactgggttt acccctgtgg ttctggcgca atgtgctggt ggcagcgacc ggctggatgg
     9061 ttggcgtcag tgtagcgctg gcgggtgcta tcggctttat tggtctggtg atcccacata
     9121 ttcttcggtt gtgtggttta accgatcatc gcgcattact tcccggctgc gcgctggcag
     9181 gggcgagcgc attgctgctg gccgatattg tagcgcgcct ggcattagct gccgcagagc
     9241 tgcctattgg cgtggtcacc gcaacgttgg gtgcgccggt gtttatctgg ttattgttaa
     9301 aagcaggacg ttagccgcaa aaagacggtc tatgattaaa agctaagatt tttactgacc
     9361 acacccagga gaaacgatgc aagattccat tctgacgacc gtagtgaaag atatcgacgg
     9421 tgaagtgacc acgctggaga agtacgccgg taatgtgctg ttgattgtca atgtcgcctc
     9481 aaagtgtggc ttaacgccgc aatatgagca gttggagaat attcagaaag cctgggccga
     9541 tcgaggtttt gtggtgctgg gattcccgtg caaccagttt ctggaacaag aaccgggcag
     9601 cgatgaagag attaaaactt actgtaccac cacatggggg gtgacgttcc cgatgttcag
     9661 taagattgaa gttaatggcg aaggacgcca tccgctgtat caaaaattga tttccgcagc
     9721 gccgaccgca gtcgcgccgg aagagagcgg attctatgcc cgtatggtca gcaaaggtcg
     9781 tgcaccgctg taccctgatg atattttatg gaattttgag aaatttttag ttggcaggga
     9841 cggattggtc atccagcgtt tttccccgga tatgacgccg gaagatccca ttgtgatgga
     9901 aagcattaaa ctggcgttgg caaaataatg tctattgtga tgcagttaca ggatgttgcg
     9961 gaatctaccc gcctggggcc gctttctggc gaggttcggg ctggggagat cctgcacctg
    10021 gtggggccga atggcgcggg taagagtacc ttactggcgc gaatggccgg aatgaccagc
    10081 ggtaagggaa gcattcagtt cgcggggcaa ccactggaag catggtccgc aacaaaactc
    10141 gcgctgcatc gtgcctatct ttcacaacag cagacgccgc cgtttgcaat gccggtctgg
    10201 cactacctga cactgcatca gcacgataaa acgcgtaccg aactactgaa tgatgtcgca
    10261 ggggcgctgg ctcttgatga caaactcggt cgtagcacca atcaactttc cggcggtgaa
    10321 tggcaacgcg tacgtcttgc tgcggtggtg ctgcaaatca caccacaagc caatcccgca
    10381 ggccaattgc tgcttcttga tgagccgatg aacagtcttg atgttgcgca acaaagtgcg
    10441 ttagacaaaa ttctgagcgc gctgtgtcag caaggactgg cgattgtgat gagcagtcac
    10501 gatctcaacc acacattgcg tcatgcgcat cgggcgtggt tgctaaaagg tggaaaaatg
    10561 ctggccagtg gacgcagaga agatgtgctc acgccgccaa atctggcgca ggcctatggg
    10621 atgaattttc gccgtctgga tatcgaaggt cacagaatgc tgatttcgac catctgattt
    10681 cattagtatc ttgaaaatcg ggtaattacg acgctaaatt aatgcaagaa taaaaaacag
    10741 aggaaccgta cggaatgcgt ttctgcctta ttttgatcac agcactgttt ctggccgggt
    10801 gtagccacca taaagcaccg ccgccaaatg ccagactttc tgattcgatt accgttattg
    10861 ccggtttgaa cgaccagcta caaagctggc atggcacgcc gtatcgttat ggtggcatga
    10921 cgcggcgcgg tgtggactgt tcgggatttg tggttgtgac gatgcgcgat cgtttcgatt
    10981 tgcagctgcc ccgagaaacc aaagaacaag cctctatcgg cacgcaaatt gataaagacg
    11041 agttgctgcc tggtgacctg gtctttttca aaacgggttc cggacaaaat ggtttgcatg
    11101 taggtattta cgataccaac aaccaattta tccacgcctc taccagcaag ggagtgatgc
    11161 gttcctcact tgataatgtc tattggcaga aaaatttctg gcaggcgaga cgaatctagt
    11221 atgtcgaaaa ttggacgtcg atgatacatc gccgtccatt tactccagaa tacagtagta
    11281 ttttgcagac aaaaattccg taaatggatt attcgtttat aatcaggata atctaaaaaa
    11341 aggacccctg aaaaagtggt taacatggaa caaatgaaag ttactccttc tgtaattgtt
    11401 atgtcttatg gcctcatccc ttttagccgg atactgaaaa acatccttcg agagggacgg
    11461 ttaccatgaa gatttttttg gagaatcttt atcattctga ttgctatttc ctgccgataa
    11521 gagataacca gcaagtcctt gtcggcgttg agttaattac ccatttctcc agtgaagatg
    11581 gcacggtgcg tatcccgacg agtcgggtta tagcgcaact taccgaggag caacattggc
    11641 aattattttc agagcagttg gaattactaa aatcatgcca acattttttt attcaacata
    11701 aactctttgc ctggcttaat ttaacgccac aagtggctac attattatta gagagggata
    11761 attacgcagg cgaactatta aaatatccgt ttattgaact gttgattaat gagaattacc
    11821 cgcatctcaa tgaaggaaaa gataatcgcg gtcttttatc cttatcgcaa gtgtatcccc
    11881 ttgtgttagg taatttggga gcaggcaaca gcacaatgaa agcggttttt gatggtcttt
    11941 tcacccgcgt aatgctggat aaaagtttta ttcagcaaca aatcacgcat cgctccttcg
    12001 aaccgtttat tcgtgcgatt caggcgcaaa tttccccttg ttgcaattgc atcatcgcgg
    12061 ggggcattga tacgccagaa atattagcac agattacccc cttcgatttc catgctctcc
    12121 agggatgcct gtggcctgcc gttcccataa atcagataac gacgttggtt cagcgataac
    12181 cctcctgttt gctggtgttt aagacgagag taaccgtcta cactatcaaa caggaggctc
    12241 tatgaccctg tcttttatta cccgctggcg cgatgaattg ccagaaacct atacagcact
    12301 ttcccctacg cctttaaata atgcccggct gatttggcat aataccgaac tcgctaacac
    12361 gctgagtatt ccatcgtcgc tgtttaaaaa tggcgcaggt gtctggggcg gcgaaaccct
    12421 actgcctggt atgtcaccac tggcgcaggt ttacagtggt catcagttcg gcgtctgggc
    12481 gggccaactg ggtgatgggc gcggcatttt acttggcgaa caactgcttg ctgatggcac
    12541 tacaatggac tggcatctga aaggtgctgg cctgacgcct tattcgcgaa tgggtgatgg
    12601 acgggcggta ttacgttcga cgatacgaga aagtctcgcc agtgaggcga tgcattatct
    12661 gggcattccg acgacccgcg cgttaagtat cgtcaccagc gattcgccag tgtatcggga
    12721 aacggtggag ccaggcgcga tgctgatgcg tgtggcacca agtcatctgc gctttggtca
    12781 tttcgaacat ttttactatc gccgcgagcc ggataaggtt cgtcagttgg ctgactttgc
    12841 cattcgtcat tactggtcac atcttgaaga tgatgaggac aaataccgtc tctggtttaa
    12901 cgatgttgtc gcacgtaccg catcgttaat tgcccaatgg cagacggtcg gctttgcaca
    12961 tggggtgatg aataccgaca acatgtcgct gctgggactg acgcttgatt acgggccgtt
    13021 tggttttctt aatgattacg aacccggttt tatttgtaat cactcggatc atcaaggacg
    13081 ttacagcttt gacaatcaac ctgctgtcgc gttgtggatt ttacagcgtc tggcgcagac
    13141 attgtcacca tttgttgccg tagatgccct gaatgaggcc ctggacagct atcagcaggt
    13201 tttgttgacg cattatggac aacggatgcg gcagaaactg ggctttatga cggagcaaaa
    13261 agaggataac gcgctactga atgaattatt cagtctgatg gcgcgagagc gcagcgatta
    13321 tacccgcaca tttcgcatgc tgagtctgac cgagcagcac agcgcggcgt caccgctacg
    13381 tgatgagttt attgatcgtg cggcatttga tgactggttt gcccgttatc gagggcgttt
    13441 gcaacaagac gaggttagcg atagtgagcg tcagcaactg atgcaaagcg ttaaccccgc
    13501 tctggtattg cgcaactggt tggcgcaacg ggcgattgaa gcggcagaaa agggtgatat
    13561 gacggaattg caccgcctgc atgaggcgtt gcgaaatcct ttcagcgaca gagatgatga
    13621 ctatgtcagt cgtccacctg actggggtaa acggctggaa gtcagctgct cgagttaaag
    13681 gctacttggt caacagcagc ttgccagcct gagttttacg gagcagatat tcttgcccgt
    13741 catgatcgat aatcagtttg ccatccggac ctaacagtgt ctggctggaa attcgccgaa
    13801 taggctgcgg ggaagcggtc ttgtgattgg catccgtttc aggcgtgagt tttctgctat
    13861 ccgtataacg cataaaaatt aataataatt gatataacaa tgattcttat tatcaatatt
    13921 atggctgacc agagcaagca gtaatgtgtt gagaagacaa caagttgcgc agaaatgccc
    13981 atcccatcag gaatgggcac gcattcagaa gcgggtatct accgcagagg ctagtttttc
    14041 gaccaggcgt tcggtatctt cccagcccag acacgggtcg gtaatggatt gaccgtaagt
    14101 gagcggctga ccgccgacga ttttttgcgt tccttcgcgc aggaaacttt ccgccataat
    14161 tccagcaatc gccgtagagc cattgcggat ttgctgacaa atatcctcac aaacttctaa
    14221 ctggcgacgg tgctgcttct ggcagttacc gtggctgaaa tccaccacca gatgttcagg
    14281 taaatcaaac tcgtgcagcg tatcgcaggc tgcggcgata tcatcggcat gataattcgg
    14341 ttttttgccg ccacgcataa taatgtggcc atacgggttg ccgctggtct gatagatggt
    14401 catctgacca tttttgtctg gcgagaggaa catatggctg gcgcgggctg cgcggatagc
    14461 atccacagca atccgcgtat tgccatcggt accattttta aaacctaccg gacaggagag
    14521 tgccgaagcc atttcacggt ggatctgact ttcggtagta cgtgcgccaa tcgcgcccca
    14581 actgattaaa tcagcaataa actgaccggt caccatatcg aggaactcag tcgcggttgg
    14641 gacgcccagc tcatttacct gtaaaagtaa tttgcgcgcc agctccagac cgtgatttat
    14701 ccgatagctg ccgtttaaat ctggatcgga gattagtcct ttccagccga caacagttcg
    14761 tggtttttca aaataggtgc gcattacgat ttccagccgt gactggtact ggttgcgcag
    14821 cgactgcaga cgggtggcgt actccattgc agcggtgaga tcgtggatcg agcaggggcc
    14881 aataatgacc aacagtcgct tatcttcacc attgagtatt ttttcaattc tgcggcggga
    14941 gtcggtgaca tgggtggcga cgccaggcgt tacgggatac cgtagcgcga gttcggcggg
    15001 cgttaccagg ctctcaatac gcgcagtacg gagttcgtca gttctgttca ttacaagtct
    15061 cagaaatttg ttgcttctac ggcaggactg ccgggaagtg atgcgcatca cgataaacca
    15121 atcgctgctg atttcaagtg cgaggtgtgt cacacctcgc cgggtggtta gcatgataac
    15181 aaaaaaataa gctaacgcac tagttctcta gtacattcgg cgactaaggc ccatgatatc
    15241 gaggatcttg gtggcaatct cttctaccga ataattggta ctgttaatcc acgggatctg
    15301 atttttacgg tacaaggctt ccacttccgc gacttccatc ctgcactgac gaagcgaggc
    15361 atagcgactg ttctcccgac gttcctcgcg aatcgccgcc agacgttccg ggtcgatagt
    15421 caggccgaac aatttatgct gaagcggttt gagcgacgcg ggtagcacca gattatccat
    15481 atcgtcggca ataaaggggt agtttgccgc gcggatacca aattgcattg ccagatacag
    15541 actggtgggg gttttaccac agcgagaaac accgagcagg atcacctgag cctgatccag
    15601 attgcgcaac gaaatgccgt catcgtgggc gagggtgtaa tcaatcgccg caatgcgcgc
    15661 atcatattta ttgagattat tagggttaag gccatgggta cgatgagcaa tcggcgttgg
    15721 atccagtttc atctcttgtt gtagcggggc aaccagcgcc tgaacgatat cctggcaaaa
    15781 accttcactt tgcaagatga tggcgcgaat ttccggcaac acgatggagt agaagaccag
    15841 cggacgcacg cctgtctggt gataaattgc gtcaatctga tccttcactg cccgtgcacg
    15901 gctctcattt tcgacaaacg gcagcgtgat gctgctgata gtgacgggaa attgtgacat
    15961 tactgcgtgt cctaatacct ccgcagttat tgccgtacca tcagaaatat aaaaaacgtg
    16021 gcgatcaaca gcattatcca ttttgttctt cccgtgatgc agacataatt ctatgaaagc
    16081 ataaattaaa aacgcacaga agcgtagaac gttatgtctg gtttataaaa tgaaccttca
    16141 attttatttt ttatgaaaac agcatttcat ttttatggtt tcgtttatac cgatggttta
    16201 tgtggaaatt gtcgaaaa
//
LOCUS       DBFIFM010000105        16202 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.105, whole genome shotgun sequence.
ACCESSION   DBFIFM010000105 DBFIFM010000000
VERSION     DBFIFM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.105"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(269..694)
                     /gene="arsC"
                     /locus_tag="N7C74_002832"
     CDS             complement(269..694)
                     /gene="arsC"
                     /locus_tag="N7C74_002832"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HGU2432666.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTTLG
                     TRLCRPSEVVLEILPDAQKGAFTKEDGEKVVDEAGKRLK"
     gene            complement(707..1996)
                     /gene="arsB"
                     /locus_tag="N7C74_002833"
     CDS             complement(707..1996)
                     /gene="arsB"
                     /locus_tag="N7C74_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HGU2432667.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2050..2403)
                     /gene="arsR"
                     /locus_tag="N7C74_002834"
     CDS             complement(2050..2403)
                     /gene="arsR"
                     /locus_tag="N7C74_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HGU2432668.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPSWAAKIIEQAWRCEQEKVQVIVRN
                     LARQNCSVDSKNTCS"
     gene            3147..3230
                     /gene="dinQ"
                     /locus_tag="N7C74_002835"
     CDS             3147..3230
                     /gene="dinQ"
                     /locus_tag="N7C74_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HGU2432669.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3284..4636)
                     /gene="gorA"
                     /locus_tag="N7C74_002836"
     CDS             complement(3284..4636)
                     /gene="gorA"
                     /locus_tag="N7C74_002836"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HGU2432670.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4708..5550)
                     /gene="rlmJ"
                     /locus_tag="N7C74_002837"
     CDS             complement(4708..5550)
                     /gene="rlmJ"
                     /locus_tag="N7C74_002837"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HGU2432671.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEPYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKSDGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5753..7795
                     /gene="prlC"
                     /locus_tag="N7C74_002838"
     CDS             5753..7795
                     /gene="prlC"
                     /locus_tag="N7C74_002838"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HGU2432672.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQVLREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7803..8555
                     /gene="rsmJ"
                     /locus_tag="N7C74_002839"
     CDS             7803..8555
                     /gene="rsmJ"
                     /locus_tag="N7C74_002839"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HGU2432673.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8604..10073)
                     /gene="dtpB"
                     /locus_tag="N7C74_002840"
     CDS             complement(8604..10073)
                     /gene="dtpB"
                     /locus_tag="N7C74_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HGU2432674.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGTFTLFYMSINIGSLIAL
                     SLAPVIADKFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            10239..10292
                     /locus_tag="N7C74_002841"
     CDS             10239..10292
                     /locus_tag="N7C74_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432675.1"
                     /translation="MVLNIVLAGDFDCERRP"
     gene            10270..10323
                     /locus_tag="N7C74_002842"
     CDS             10270..10323
                     /locus_tag="N7C74_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432676.1"
                     /translation="MIANADRRPDKAFTLIR"
     gene            complement(10390..10824)
                     /gene="uspA"
                     /locus_tag="N7C74_002843"
     CDS             complement(10390..10824)
                     /gene="uspA"
                     /locus_tag="N7C74_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HGU2432677.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11215..11550
                     /gene="uspB"
                     /locus_tag="N7C74_002844"
     CDS             11215..11550
                     /gene="uspB"
                     /locus_tag="N7C74_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HGU2432678.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11621..13120)
                     /gene="pitA"
                     /locus_tag="N7C74_002845"
     CDS             complement(11621..13120)
                     /gene="pitA"
                     /locus_tag="N7C74_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HGU2432679.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            13352..14554
                     /gene="yhiN"
                     /locus_tag="N7C74_002846"
     CDS             13352..14554
                     /gene="yhiN"
                     /locus_tag="N7C74_002846"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGU2432680.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTVRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSC"
     gene            complement(14872..15924)
                     /gene="yhiM"
                     /locus_tag="N7C74_002847"
     CDS             complement(14872..15924)
                     /gene="yhiM"
                     /locus_tag="N7C74_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HGU2432681.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4172 a         4092 c         4207 g         3731 t
ORIGIN      
        1 tccagtacgt ttttaaactg ctgcactgta tcgctgatca ctaagcagct cactgataca
       61 tcatgctgga cgcgataatt ggaatctcac gcagggagta atgtgtaaat agcaaaatcg
      121 cctgatgcgc tacgcctgtc aggcctacaa ataacataca atttattgaa ttttcatgac
      181 tttgtagatc ggataagacg cgttagcgtc gcatccgaca taaataaagc gcacttttct
      241 aacaacctgt gggggggata tagccgcttt atttcaggcg cttacccgct tcatcaacca
      301 ctttctcgcc atcttccttg gtgaatgcgc ctttttgcgc atctggcaga atttccagca
      361 ccacttctga agggcggcac aggcgagttc ccagcgtcgt caccacaatc gggcgattaa
      421 tcagaatcgg gtgctgaagc ataaagtcga ttaaccgatc gtcagtaaat ttatcttccg
      481 caaggcccag ctcctcatac ggttcgacgt ttttacgcag cagcgcgcgt acggaaatcc
      541 ccatatcggc aatgagtttg accagttcat cgcgcgttgg cggagtttcc agataatgga
      601 taatggtcgg ttctgtgccg ctgttgcgga tcatctccag cgtattacgc gacgtgccgc
      661 aggccgggtt gtgataaatg gtaatgttgc tcatatcagt atctcattac aaagtgaaag
      721 agagacgtag cgccagcgca gccagcgtca caaacagcac aggcagggtc atgataatcc
      781 ctgtacggaa gtaatatccc cagctgatag tcatattctt ctgcgaaagt acgtgcagcc
      841 agagcagcgt tgccaggcta ccaatcgggg tgattttcgg ccccaaatcg cagccaatca
      901 cgttggcata aaccatcgct tctttgataa cgccagatgc cgtgctgcca tcaatggaca
      961 acgcgccaac cagtaccgtc ggcatattgt tcataataga agagaggaag gcggtgagga
     1021 atccggtgcc gagcgtcgcg gcccacaggc cgttatccgc cagcacgttg agtacgccag
     1081 aaagatattc cgttaatccg gcattgcgca ggccataaac caccagatac atgccgagcg
     1141 agaagatgac aatctgccag ggcgcaccgc gcagcacttt acccgtatta atcgcatgac
     1201 cgcgtttagc gacgacaaat aatatcaacg cgcccactgc cgcaatggcg ctgaccggaa
     1261 tgccgagcgg ttccaggacg aaaaatccca ccagcagaag cagtaaaaca acccagccag
     1321 ttttgaacgt agcaggatct ttaatcgctt ctgcgggaga tttcagcagc gccatgtcgt
     1381 agttctgcgg aatatctttg cggaaataga gatgcaacat caccagcgtg gcaacaatcg
     1441 cggcgatatc caccggcacc atcaccgagg catattcgcg aaagccgagg ccaaagaaat
     1501 ctgcggaaac gatattcacc aggttggaga caataagcgg caggctggcg gtatcggcaa
     1561 tgaatccggc cgccatcaca aacgccagcg tagtgccttt actgaaccct aaagccagca
     1621 gcatggcgat gacaatcggt gtcaaaataa gcgccgcgcc atcattggca aacagggcgg
     1681 caacggcagc accgagcagg acaatccagg taaacagcaa gcgaccgcgt ccattgcccc
     1741 agcgtgaaac gtgcagtgcc gcccattcaa aaaagccgga ctcatccagc agcaggctga
     1801 tgataataac ggcaataaac gcagccgtcg cgttccagac gatattccac accaccggaa
     1861 tatcgcccgg atgtactacg cccgtaacta acgccagtac tgcgccgagc gttgcactcc
     1921 agccgatgcc taaacctttc ggctgccaga taaccaatac aatggtcagg acaaagatag
     1981 cgcctgccag taacataatg cctcccggaa aaacacatgt gcaaatccat atgtgtttag
     2041 ataaattttt taactacaag tgttcttact gtctacggaa caattttgtc tggccaggtt
     2101 gcggacaatc acctgaacct tttcctgttc gcatcgccag gcctgctcaa taattttcgc
     2161 ggcccatgat ggaatatgcg gtgataagcg gtaatgaacc cacttaccct gcttgcgatc
     2221 cagcaacaaa ccgctttcac gaagaagcgc cagatggcgg gagatcttgg gctgcgactg
     2281 gtcgagagca gtgcagagat cacagacgca taactctccc agctcgctga gcagtaaaac
     2341 gatgcccaga cgggtttcat cggcaagaat tttgaacagt tggatgggta acagaaatga
     2401 catattgcgc tcctgattgt tgcaggtagt gtctctcttc gaagcggata agtcaaaaac
     2461 atatatggtt taacgaatgt gtatgtgcag aggaaggtaa taggtgtgaa ttaagcgttg
     2521 gctattcatt tgaaaggagg tctgaatcag gacgtaaaaa aaccgacttt gcgtcggttt
     2581 tttactttcc agcccagagt tggtggctct ggctggagtg agaatgccca catctaacgg
     2641 ttcaatacta ttagccagtg gctaacattg caagcgttag cgatttcttc agctggagaa
     2701 gggacagttt gctatacatg ttggtgaaag aggatttata tactttcatc gggtcgctgc
     2761 cgtaccaggc tttgtccgaa tccggatgta aaaaaaccga cttcgcgtcg gtttttttac
     2821 tcttcagccc tgagttggtg gctctggctg gagtgagaat gccatcatct aacgcatcaa
     2881 tactaatacc atactgaaac tattgcaagg acatgctggt tttataacct gaatatactg
     2941 tatgattatc cagttagctc tgaggcattt tcactctggc aatgcgcata aacgctttca
     3001 aagtcctgtt cagtggtacg ggtggtgccg ttaactgatg ctctggccgg agtgagaata
     3061 ccattatcta acaatagggc atgcgccgtg acaggcgatg gatgagtaag cggatgcatt
     3121 ctcactccat cgtatggaga acacgggtga ttgataaagc aatcatcgtt cttggggcgt
     3181 taattgcgct gctggaactg atccgctttc tgcttcagct tctgaactga tagcggaaac
     3241 gtaattaagg gcaaagagca caccactctt tgccctttaa catttaacgc atggtcacga
     3301 actcttctgc cgccgttggg tgaatggcga cggtattatc gaagtctttt ttggttgcgc
     3361 ccatcttcag tgccaccgcg aagccctgca acatttcgtc cataccaaaa ccaatgccgt
     3421 gaataccgac aatcttctct tctggcccaa cgcacaccag cttcatgcgg cacggctggc
     3481 ggtgagtggt gacggcggta tacatcgcgg tgaaagagga tttatacact ttcacctgat
     3541 cgtcgccgta ctgctcgcgc gcctgcggtt ccgttaaacc aacagtacca atcggcggat
     3601 ggctgaagac cacggtcgga atgttgctgt aatccagatg ctcatccggc ttgttattaa
     3661 acaggcgttc agagagacgg cgacccgctg caactgccac cggtgtcagt tctactgcac
     3721 ccgtgttatc gcccaccgcg taaatacctt caacgttggt gttttgatat ttatcgacga
     3781 cgatatagcc tttttcgttg gttttaacgc cagcggcttc gaggttgatg ttgtcattgg
     3841 caggctcgcg gccaatcgcc caaatcaggc aatccaccgt ttcactgcga ccatcttcca
     3901 gctccagcgt caggctacca tcggcatttt tcactaccgc tttcgggatg gcgttggtgt
     3961 gcaactgcgg gccttcggcg ttcatcactt cgaccagcgt ttcagagatc atcgggtcga
     4021 agctgcgcag cggcgcatgt ttacgcacaa acagatgcgt tttcgcgccg aggccgttaa
     4081 tcacgccagc cagctcaacg gcgatgtaac ccgcgccaac aaccgccaca cgctctggca
     4141 aagcaggaag ggcaaagaaa ccatcagaat caataccgta ttccacgccc ggaatatccg
     4201 ggtggctcgg acgaccgccg gtggcgatca gaatatgatc ggctgtgatg gtttcaccgt
     4261 taacttccag cgttttggca tcaacgaagc gggcaaagcc tttgattaca tcaacgttat
     4321 ttttaccgag cacgttttca taggaagtat gaatacggtc gatataggcg gtacggctgg
     4381 cgatcaacgt ttcccagttg aatttattga tagtggtatc aaaaccataa tccgggccgt
     4441 acatatggat cgcttcacgg atttgcgccg cgtgccacat cactttcttc ggtacgcagc
     4501 cgacgttcac gcaggtgccg cccagctctt tggcttcaat cagcgcgcat ttctgaccgt
     4561 acatagccgc gcggttgatg gaggcgatac cgccgctgcc gccgccgatg gcgatgtaat
     4621 catagtgttt agtcatgaca gagtgtcctt atcgttgatt accgcgattg tagcgcgaga
     4681 cgtaataggt gccagcaatg gctgcaatta ctctggcacg atccagctta cggtggcgtg
     4741 cccggtgcca gctggaacca gtttgctgtg cagccacggc agcacgttat tcatctgctg
     4801 ttccagtttc cacggcgggt taatcacaat catgccggaa gcggtcatgc cacggcgatc
     4861 gctgtctggc agtaccgcca gttcaatttg cagaattttg cgaatgccgg tcgcttccag
     4921 gtcgtggatc atgcgcttaa tttgctggcg cagcaccacc ggataccaca atgcgtaagt
     4981 gccagtggcg aaacgtttgt aaccttctgc tatcccgctg actaccgcct gatagtcggt
     5041 tttcatttca tacggcggat cgataaggat taaaccacgg cgagaaaccg gcggcagttt
     5101 ggccttaagc tgctggaaac cgtcggattt ttcgacgcgc gcacggctgt ctttctgaaa
     5161 ttcagaacgc agcaacgggt aatcgctcgg gtgcagttcg gtcagttgca ggctgtcctg
     5221 ttcacgcagt aactgacggg caatcaacgg tgaaccgggg tagtaacgca actgaccgct
     5281 acggttgaag tgttttacca cattgatgta cggctccagc tccgcaggca aatcgtcctg
     5341 ctgccagata cgggcgatac cttcgagata ttcgccggta cgctcggcat gttcgctgcc
     5401 taactgataa cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt tatctttctc
     5461 tttcagcgac tcgatgatca ggctctgaac ggtatgttta aggacgtcgg cgtggttgcc
     5521 agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt gttccggtaa gtaaaaatcg
     5581 gcccgtattc ggggcgcaca aaagcctatc aggacagtat accgaaaaga ggccgccgcc
     5641 gcgaaagcgt aacgtttctc attgaaattc actacactta accccatgct acaaacatta
     5701 tgtaaagcgc ctgttgagcg cttccttaac ctctttaacc aggactgcgc taatgacgaa
     5761 tccgttactg actccctttg aattgcctcc gttttctaaa attctcccgg aacatgtcgt
     5821 tccagccgtg actaaggcat tgaatgactg ccgcgaaaat gtggagcgcg tagtagcgca
     5881 aggagcaccg tacacctggg aaaatctctg ccagccgttg gcggaagtgg acgatgtgtt
     5941 ggggcgtatt ttctccccgg tcagccacct gaactcggtg aaaaatagcc cggaattgcg
     6001 cgaagcgtac gaacaaactc tgccgctgct gtcggaatac agcacctggg tagggcaaca
     6061 tgaagggctg tataaagcgt accgcgacct gcgcgatggc gatcattacg ccacgctgaa
     6121 cacggctcag aaaaaagcgg ttgataacgc actgcgcgat tttgaactct caggcatcgg
     6181 tctgccgaaa gagaaacaac agcgttacgg cgaaattgcc actcgtcttt ctgaactggg
     6241 caaccagtac agcaacaacg tccttgatgc gacgatgggc tggactaaac tcgttaccga
     6301 cgaagcggaa ctggcgggga tgccagaaag cgcgctggct gcggcaaaag cccaggccga
     6361 agcgaaagag ctggaaggct acctgttgac gctggatatc ccaagctact tgccggtaat
     6421 gacctactgc gacaaccagg tcttgcgtga agagatgtat cgtgcttaca gcacccgcgc
     6481 ttcagatcaa ggtccaaacg ccggtaaatg ggataacagt aaggtgatgg aagagatcct
     6541 cgcgctgcgt cacgaactgg cgcaactgct gggctttgaa aactacgcgt ttaaatcact
     6601 ggctaccaaa atggcggaaa acccgcagca ggtgcttgat ttcttaaccg atctggcaaa
     6661 acgcgcgcgt ccgcagggcg aaaaagagct ggcgcaactg cgtgccttcg ccaaagccga
     6721 atttggcgtg gatgagttgc aaccgtggga tatcgcatac tacagcgaaa aacaaaaaca
     6781 gcacctctac agcatcagcg acgaacaact gcgtccgtac ttcccggaaa acaaagcggt
     6841 taacggcttg tttgaagtgg taaaacgtat ttacggcatc accgctaaag agcgtaaaga
     6901 tgttgatgtc tggcacccgg atgtacgttt cttcgaactg tatgacgaga acaacgaact
     6961 gcgcggcagt ttctacctcg acctgtatgc ccgtgaaaac aagcgcggcg gggcgtggat
     7021 ggatgactgc gtaggccaga tgcgtaaagc cgatggttcg ctgcaaaaac cggtcgcgta
     7081 tctgacctgt aacttcaacc gcccggtgaa tggtaaaccg gcgctgttca ctcacgacga
     7141 agtgatcact ctgttccacg agttcggtca cggcctgcac catatgctga cccgcatcga
     7201 aaccgccggt gtttccggta tcagcggtgt gccgtgggat gcggtcgaac tgccgagtca
     7261 gtttatggaa aactggtgct gggagccgga ggcgctggcg tttatctctg gtcactacga
     7321 aaccggcgaa ccgctgccga aagagttgct ggataaaatg ctggcggcga agaactacca
     7381 ggcggcgctg tttattctgc gccagctgga gttcggcctg ttcgatttcc gcctccatgc
     7441 cgagttccgc ccggatcagg gagcgaaaat cctcgaaact ctggcagaaa tcaagaaact
     7501 ggttgccgta gtaccgtctc catcctgggg ccgtttcccg cacgctttca gccatatttt
     7561 cgccggtggt tatgccgcag gttactacag ctacctgtgg gctgacgtac tggcggcaga
     7621 tgctttctcg cgctttgagg aagagggcat tttcaaccgt gaaaccgggc agtcgttcct
     7681 cgacaacatt ctgagccgtg gcggttcaga agagccgatg gatctgttca aacgcttccg
     7741 tggtcgtgaa ccgcagctgg atgcgatgct ggagcattac ggcattaagg gctgatcatt
     7801 cagtgaaaat ctgcttaatt gatgaaacag gcaccggaga cggtgcctta tctgttctgg
     7861 cggcccgctg ggggctggag cacgatgaag acaacctgat ggcgctggtg ttaacgccgg
     7921 aacatctgga attgcgcaag cgtgatgagc caaaacttgg cggcatcttt gttgattttg
     7981 ttggcggagc gatggcgcac cgacgcaaat tcggcggtgg tcgcggtgag gcggtggcga
     8041 aagcggtggg cattaaaggc gattatttgc cggatgtggt ggacgctacc gcagggctgg
     8101 ggcgcgatgc ctttgtgctg gcttcggtcg gctgccgcgt gcggatgctg gagcgtaatc
     8161 cagtcgttgc cgcgctactc gacgacggcc tggcgcgtgg atatgcggat gcggaaatcg
     8221 gcggctggtt gcaggagcgt ttgcagttaa ttcacgcctc cagcctgacg gcgctgaccg
     8281 atattacccc gcgtccgcag gtggtctatc tcgacccgat gttcccgcat aagcagaaaa
     8341 gcgcgctggt gaaaaaagag atgcgcgtct ttcagtcgct ggtgggaccg gatcttgatg
     8401 ccgatggatt actggagcct gcacgcttgc tggcgaccaa acgcgtggtg gtaaagcgcc
     8461 cggactacgc gccaccgcta gcgaatgtcg ccacgccaaa cgcggttgtg actaaagggc
     8521 atcgctttga tatctatgca ggtacgccag tgtaaaagta aagcagggta gcgataacgc
     8581 taccctgctt tctgcaagaa taattaatgg ctttccggcg tcgcaatcat gcgtttcagc
     8641 cacggcacca tcaacagcat cactactgca acgcccagcg tgaccagacc aatcttacca
     8701 aacacgttgg tatagacagg caacgtctca agcggatcgg taatgttgtc tggtactgcg
     8761 gtaaatgttg ccacatagcc gcccagcaag aacgcggcag cctgtgtcag gaaccacatc
     8821 ccgagaataa agcccatcaa atgctgcggc accagagcag caatcatcgc caggccaagg
     8881 gcgctaataa acagttcacc taagctctgg aataagtaca ccagcacgat aaaccatggc
     8941 gatgtcagcc cttgtgcatc cgcaaaccac attcccgcag ctgccgccgt caaaaagccc
     9001 agtgagcaca taaacatgcc gagagtaaat ttcatcggca tcgagaggtc tttgcctttg
     9061 ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac ccagaacggg
     9121 ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg atgcacgttg
     9181 ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta aaacaccacc
     9241 gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt atccagcttg
     9301 aatgcctgac gaaagaagat gatggtgacg acgatggaga gaacaatcag caccagattg
     9361 gcgacttcta cgttgtgcat cagccaggcg cagacgaaga tcatcaccac gctgccaagt
     9421 aatacgtaca acagtttgct gaagctcatt ggccggaagt cgggctcaga accaatgtct
     9481 ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc cgcaccgcac
     9541 aggttgtagg tgactgaata gccgaattta tcagcgatca caggggccag cgataacgct
     9601 atcaacgagc cgatattgat cgacatatag aacagggtga atgtgccatc aaggcgcgga
     9661 tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa caggccgtta
     9721 ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag cgacatgccg
     9781 gtcatgaagt agccaatcgc cagcacaagt gctccaagaa cgatggtgcg tttggtcccc
     9841 agcaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag tgcagcaaaa
     9901 gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac gaagaaaacc
     9961 gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac aaaaaagatc
    10021 atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt catatttatt
    10081 aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc gaaaaagttt
    10141 acatctgtca gagattctta atattgatgc ggtgaataga ttaatacact attacaacag
    10201 aaaataacca gctgatcaca ctaatgatgg aaaatatgat ggtgttaaat attgtattgg
    10261 ctggggattt tgattgcgaa cgcagaccgt aggccagata aggcgtttac gctgatcagg
    10321 taacaaggcg cgacaaaaag caaaacgccc gcatatgcgg gcgttcagga acacgtcgtg
    10381 gagggaagat tattcttctt cgtcgcgcag cggaacaatc agcatatcaa cgtgaacggt
    10441 gttgatcagc tgacgtgcgg aagacatcag tttgctccag aagtcctggt ggtgaccaca
    10501 aaccactaga tccatatcgt atttcttgat tgcatcgacc agaacctggc ccaggtcgcc
    10561 gctgccgctc agggtttcag tgattgggta gcctgcatta gtggaaagct cggtcagcgc
    10621 atgatgtgtc tcttcagaga tgcgtttctg catatcacca agattcacat caataagccc
    10681 ggtatatagg tcagagtagt ttacatctac gtggatcaga gaaactttcg cattgtatgg
    10741 gcgagccata gagactgctt tctctaccag aactttgctt tccggggaga gatcgaccgc
    10801 gatgagaatg tgtttataag ccatagtgtt actccttcca taaagttgtc gatgactggc
    10861 cagctagcgt ttcttgtgcc gttacgtcac ccgcgtcctg cgaactacgt gcgcggggct
    10921 aacccagccg taaagattca gtgttaagac catccttacc ttatagcgac ccggatgatc
    10981 cgtcaatccg ccttgcttac ccgttagata aacaaatttt cgacatgaat atattgatag
    11041 tggttaacct tctggaaaaa aaacaacctg atctcctaca ctatctatag agccgctcgg
    11101 atgttggtca ccacaaaagc ggtttctcca ggacctggtg ttaactgatt ggcagcgtat
    11161 cgggagcggt agccccgggg tggaactccg cgggcaggtc gccggggagg aggtatgata
    11221 agcaccgtcg cattattttg ggctttatgt gtcgtttgca ttgttaacat ggcgcgctat
    11281 ttctcatcac tacgcgcgtt gttagtggta ctgcgtaact gcgatccatt gctctatcaa
    11341 tatgttgatg gagggggctt ttttacctca catggccaac ccaacaaaca ggtgcgtctc
    11401 gtttggtata tctatgccca acgttatcgc gatcatcacg atgatgagtt tattcgccgc
    11461 tgtgagcggg tgcgtcggca gtttattctg accagcgcat tgtgtggtct ggtggtggtc
    11521 agcctgattg ccttgatgat ttggcattaa gcacttcagc aataataacg cataaaaaaa
    11581 gcgggccagt aaactgaccc gctcgttttg gtgcgtacta ttacaggaac tgcaaggaga
    11641 gccagtaaag cccgccggaa agcagtaccg cagccggaag ggtaaacacc caggccatca
    11701 gaatgctggt cacggtttta cgctgtaagc cgccaccatc taccaccatc gtccccgcga
    11761 cagaagagga gagtacgtga gtagtggaaa ccggcatccc ggtataactc gccaggccga
    11821 tagacactgc cgcagtcatc tgggcagaca tcccctgagc gtaggtcatg cctttcttac
    11881 cgattttctc accgatagtc gttgccacac gacgccagcc aatcatcgta ccgataccta
    11941 acgccagcgc gaccgccatg atgatccaca ccggtgcata ctcgatggtg ctaagcatgt
    12001 cggacttcag tttcttcaac aggcgctgat cgtcagcact cacgccaggc atcttcacca
    12061 ctttgtcgat agtgtcagaa acgcacagca taatgcggcg catctggcta cgttgatcaa
    12121 gcgacagctt gtcgtagctt tccacatcgg tggtcaacat acctttcagg cggttgagcg
    12181 cgttaatggt attcgacgga tggcagtgga actccgcagg ttgcgttgcg ccagcttccg
    12241 gagccggtac taactgatca gcaccagtag cttgtttcag cagcgcagga tgctgctcaa
    12301 agtaagcttc gacgttgttg atggcatcac gggtacgggt gatttcgtag ccagtggcat
    12361 tcatgttcac cacgaagcct gctggtgcga caccaatcaa taccaacata accagaccaa
    12421 tgcctttctg accatcgttt gcgccgtgcg aaaacgccac gccgatagcg gaaaggatca
    12481 gcgcgatacg cgtccagaac ggcggctttt tcttgccgtc tttcttttca cgctccgctg
    12541 gggtcaggtg gatacgggcg cgtttcttgg tgccgctcca gtagcgacgc agcaagaaaa
    12601 tcagaccgcc agcaaacacc aggccgacaa taggggaaac gatcagagaa ccgaaaatac
    12661 ttaatacttt cgggatattg agtgcatcca ccactgacgt cccggtcatc aacgcattgg
    12721 ttaaaccaat cccgatgatc gcgccaatca gcgtatgaga gctggatgca ggcaaaccaa
    12781 agtaccaggt acccaggttc cagataatcg ccgccagcaa catagagaac accatggcaa
    12841 ggccatgaga cgatcccata ttaagcagca gatccgtcgg cagcatatgc acaatggcat
    12901 aggcaacact cagaccaccc agcaaaacac ccaaaaagtt gaacaccgct gccataacca
    12961 cggcgagctg agaacgcatc gcgcgggtat agataacggt tgccacggcg ttggctgtgt
    13021 catggaaacc attgatggct tcgtagaaca gcacaaaagc cagtgcaagc aataataaca
    13081 gcccggtatg caaatccagg ccagcaaaca aatgtagcat aggacgttac gccattttga
    13141 ggacatgaac gcggcgcatt atcagtgact ttcgcggcgc gggcaaagtg aaatatagac
    13201 tttttttgat ttgcctcctg tatggatttc actcaaaaaa taattatctt atataattca
    13261 ggcaaatact tccttttagt aatattgatg ctggtgcgac cactgaggaa tctttacaat
    13321 tcacgcccgt tttttctaag aggagcgcaa cgtggaaagg tttgatgcca ttattatagg
    13381 cgctggtgcg gcgggtatgt tctgttctgc gctggcaggt caggcaggac gccgggttct
    13441 gctgatcgat aatggtaaaa aaccagggcg caaaatcctt atgtctggcg gtgggcgctg
    13501 caactttacc aacctttatg tcgaaccggg cgcttatctg agccagaatc cgcatttttg
    13561 taagtctgca ctcgcgcgtt ttacccagtg ggatttcatt gatctggtca ataaacacgg
    13621 catcgcctgg cacgagaaaa cgttagggca actcttctgc gatgactccg cgcagcagat
    13681 tgtcgacatg ctggtggatg agtgcgagaa gggcaatgtg accgtcagat tgcgtagcga
    13741 agtgctgagt gtggcgaagg atgatacagg cttcacgctt gaactgaacg gcatgactgt
    13801 cggttgtgaa aagctggtca tcgcgaccgg cgggctgtca atgccgggcc tgggcgcgtc
    13861 gccgtttggt tataagattg ccgaacaatt tggcctcaac gtgctgccga cccgcgcggg
    13921 tctggtgcca ttcactctgc ataaaccgtt gctcgaagag ttacaggtgc tggcgggcgt
    13981 ggcggtgcct tccgtgatta ccgctgaaaa cggcaccgtt ttccgtgaga acttactctt
    14041 cacccatcgc ggcttgtctg gaccggcggt gttgcaaatt tccagctact ggcaaccggg
    14101 ggaatttgtc agtatcaatc tgctaccgga tgtggacctc gaaaccttcc tgaatgagca
    14161 gcgtaacgca catccgaatc agagcctgaa aaacacactg gcggttcatc taccgaagcg
    14221 gttggttgaa cgcttacagc aactcgggca aatcccggat gtttcgctaa aacagctcaa
    14281 cgtgcgtgac caacaggcac tgattagcac attgaccgac tggcgcgtac aacccaacgg
    14341 cactgaaggc tatcgcactg ccgaagtgac gctcggcggc gtggacacca acgaactctc
    14401 ttcacggacg atggaagcgc gcaaagtgcc tgggctgtac tttatcggcg aagtgatgga
    14461 cgttaccggc tggctggggg gctataactt ccagtgggcg tggtcgagtg cgtgggcttg
    14521 tgcgcaggat ttgattgcag cgaagtcgtg ctgagctatt tttcctaaag atgaaaatta
    14581 ttagagtcgc attaaaaatg agctaatttt gatagtggct atcttgtgat tattttctaa
    14641 tgagcccgtg aactgaaccc cccatgctta atataaggtg gagggaaagg tgattgaaaa
    14701 ctcacccagt gggctgatct ttataaaata tgaacaggtt gaaaaaaaaa cagtaacttt
    14761 gctgtttttt atacttaacc actatgcatt aatgctgcgt tatttcattc tacctaagaa
    14821 aaaccagcgt tacgcaaatg gttaacgctg gttttatccg gtacgttgca attatttttt
    14881 agcagaaccc gcttctaata ttgaaacgat tgagaacaac gtaaagcata ccgctcccaa
    14941 accgaccaga acccgcgaag gaataaaata tcccatatcg gtctgcgcca tttccgcaag
    15001 aaacgatgct aggaaaaggc aaaacaggca ggtgaagacg ggaatcatcg gtattctgtt
    15061 ggctaacgaa caggtacgtc tccagaccag tgccagtagc cagacttttg agaatatgct
    15121 gtaacagatc attcccaagc aaatgaggat aataccggga gccagtcggg cgcttgcatc
    15181 agaactgact aagacgtata tgccctgcag caccgtgatt gagccgagaa aaataaccca
    15241 ataacaccac agccagtgct ctttcgttga aaacgtatta cgtgtttgat ggacgattgt
    15301 ggcaacaagg ccaattagac aggcacagat tgcggttaac ccgagcaata cgtgaccggc
    15361 gacataatgc ggagttatgt cggcactacg taacagcgta atagaccaaa tgaaaccgag
    15421 cagggtgagt aacacgggaa cggcaatgag gcaattaccg attaatgaag aataagcctg
    15481 taccggtgtt ccgtcgctct tgctccccgc tgcgttttta ggaatgagca gaaagtgacc
    15541 ggatgacgct gccaccgtcg atacacaggc ggcaatcata ccaacgccga aaataacatg
    15601 gccggcgaca aactcgtctg ccatcacatc attacctgct aacagtgccc aaccccatat
    15661 catggtgata attgaccccg catagccaat aatggggaac aatgtattgt aaaacgtatt
    15721 aacgccgcgc gtgagctgcg aaatgataat aaatgcggta gtgaataatg ccaggcatat
    15781 ggctgccaga gaaatgagca catgacccgc gacgaaatac tcgctttgcc ctgaactttc
    15841 cagcacaaag ccacctaacg cgatacaaat caagcccata attaaaggga ttaatttgaa
    15901 aagccacccg atatatatgt tcactttaaa ctcctcaggt atccctgcta atattgaaat
    15961 gtgcattaat gataatttca gcctgttaat acaatgacga gatatcatta aagacaaatg
    16021 catagcataa ctatagcggg gtttactttg cagagcgcta cttttcagcg ttcaaaaggc
    16081 catgataaat aaatatttat cctaagatta attcttattc tgttgttttt tacaattatc
    16141 ttccagggag ggattctttt tcattatttg gtaaaagacc ggatggtctc ggtgcttaaa
    16201 at
//
LOCUS       DBFIFM010000106        15767 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.106, whole genome shotgun sequence.
ACCESSION   DBFIFM010000106 DBFIFM010000000
VERSION     DBFIFM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.106"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            8..184
                     /gene="yecU"
                     /locus_tag="N7C74_002848"
     CDS             8..184
                     /gene="yecU"
                     /locus_tag="N7C74_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HGU2432682.1"
                     /translation="MIKIFIGHYINAFNSTVDGTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            171..395
                     /gene="yecF"
                     /locus_tag="N7C74_002849"
     CDS             171..395
                     /gene="yecF"
                     /locus_tag="N7C74_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HGU2432683.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEQQLALIEDEPQAAVFSKTVKQIKQAYRQ"
     gene            complement(463..1185)
                     /gene="sdiA"
                     /locus_tag="N7C74_002850"
     CDS             complement(463..1185)
                     /gene="sdiA"
                     /locus_tag="N7C74_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HGU2432684.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMETAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(1415..2167)
                     /gene="tcyN"
                     /locus_tag="N7C74_002851"
     CDS             complement(1415..2167)
                     /gene="tcyN"
                     /locus_tag="N7C74_002851"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HGU2432685.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARKLLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(2164..2832)
                     /gene="tcyL"
                     /locus_tag="N7C74_002852"
     CDS             complement(2164..2832)
                     /gene="tcyL"
                     /locus_tag="N7C74_002852"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HGU2432686.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     TAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(2847..3833)
                     /gene="dcyD"
                     /locus_tag="N7C74_002853"
     CDS             complement(2847..3833)
                     /gene="dcyD"
                     /locus_tag="N7C74_002853"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HGU2432687.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASVEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(3938..4738)
                     /gene="tcyJ"
                     /locus_tag="N7C74_002854"
     CDS             complement(3938..4738)
                     /gene="tcyJ"
                     /locus_tag="N7C74_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU2432688.1"
                     /translation="MKLAHLGRQALMGVMAVALIAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(4826..5377)
                     /gene="fliZ"
                     /locus_tag="N7C74_002855"
     CDS             complement(4826..5377)
                     /gene="fliZ"
                     /locus_tag="N7C74_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HGU2432689.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(5423..6142)
                     /gene="fliA"
                     /locus_tag="N7C74_002856"
     CDS             complement(5423..6142)
                     /gene="fliA"
                     /locus_tag="N7C74_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HGU2432690.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(6462..8219)
                     /locus_tag="N7C74_002857"
     CDS             complement(6462..8219)
                     /locus_tag="N7C74_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HGU2432691.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQATTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGETITIDL
                     KKIDSDTLGLNGFNVNGKGTITNKAATVSDLTSAGAKLNTTTGLYDLKTENTLLTTDA
                     AFDKLGNGDKVTVGGVDYTYNAKSGDFTTTKSTAGTGVDAAAQAADSASKRDALAATL
                     HADVGKSVNGSYTTKDGTVSFETDSAGNITIGGSQAYVDDAGNLTTNNAGSAAKADMK
                     ALLKAASEGSDGASLTFNGTEYTIAKATPATTTPVAPLIPGGITYQATVSKDVVLSET
                     KAAAATSSITFNSGVLSKTIGFTAGESSDAAKSYVDDKGGITNVADYTVSYSVNKDNG
                     SVTVAGYASATDTNKDYAPAIGTAVNVNSAGKITTETTSAGSATTNPLAALDDAISSI
                     DKFRSSLGAIQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGN
                     SVLAKANQVPQQVLSLLQG"
     gene            8485..9882
                     /gene="fliD"
                     /locus_tag="N7C74_002858"
     CDS             8485..9882
                     /gene="fliD"
                     /locus_tag="N7C74_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HGU2432692.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSSTTAFSATTAGNAIAGKYTISVTHLAQA
                     QTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNAKAG
                     VSASIINVGNGDYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEVSVA
                     AQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIKDWV
                     NAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVSSSS
                     YKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGSNLT
                     SWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSLNST
                     SSYLTQQFENNSNSK"
     gene            9907..10317
                     /gene="fliS"
                     /locus_tag="N7C74_002859"
     CDS             9907..10317
                     /gene="fliS"
                     /locus_tag="N7C74_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HGU2432693.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVETLMRNIADAWKESLLSPSLIQDPV"
     gene            10317..10682
                     /gene="fliT"
                     /locus_tag="N7C74_002860"
     CDS             10317..10682
                     /gene="fliT"
                     /locus_tag="N7C74_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HGU2432694.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            10760..12247
                     /gene="amyA"
                     /locus_tag="N7C74_002861"
     CDS             10760..12247
                     /gene="amyA"
                     /locus_tag="N7C74_002861"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGU2432695.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPNEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRNYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVL"
     gene            complement(12281..12694)
                     /gene="yedD"
                     /locus_tag="N7C74_002862"
     CDS             complement(12281..12694)
                     /gene="yedD"
                     /locus_tag="N7C74_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HGU2432696.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            12881..14086
                     /gene="yedE"
                     /locus_tag="N7C74_002863"
     CDS             12881..14086
                     /gene="yedE"
                     /locus_tag="N7C74_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HGU2432697.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVAAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            14083..14316
                     /gene="yedF"
                     /locus_tag="N7C74_002864"
     CDS             14083..14316
                     /gene="yedF"
                     /locus_tag="N7C74_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HGU2432698.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            14425..15093
                     /gene="yedK"
                     /locus_tag="N7C74_002865"
     CDS             14425..15093
                     /gene="yedK"
                     /locus_tag="N7C74_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HGU2432699.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEISGKEASEIAASGCVPANQFSWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            15204..15683
                     /gene="yedL"
                     /locus_tag="N7C74_002866"
     CDS             15204..15683
                     /gene="yedL"
                     /locus_tag="N7C74_002866"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU2432700.1"
                     /translation="MFTIKTDDLTHPAVQALVAYHISGMLQQSPPESSHALDVQKLRN
                     PTVTFWSVWEGEQLAGIGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAHD
                     RCLHRLSLETGTQAGFTACHQLYLKHGFVDCEPFADYQLDPHSRFLSLTLCEDNELL"
BASE COUNT         3897 a         4008 c         3879 g         3983 t
ORIGIN      
        1 aacattcatg ataaaaatat ttattggtca ttatattaac gcatttaata gcaccgttga
       61 cggcacgctc aaaaaacaac cactgctatt tttagcaaag cttatggtat actccgccgc
      121 cttaacattt ttcaccgcaa attttcattg caacatgacg aggaaaataa atgagtacgc
      181 ctgatttttc tactgccgag aataatcaag aactggcaaa tgaagtctcc tgcctgaaag
      241 cgatgctgac gctgatgctg caggcgatgg gacaagctga cgcgggccgc gtgatgttaa
      301 agatggaaca acagcttgcg ctgatcgaag acgaacccca ggctgcagta ttttccaaaa
      361 cggttaagca aattaaacaa gcctaccgtc agtaatataa aaccggctga tagcgtgcct
      421 ttcagccggt ttttgcatct ggcacgcagg acagaaaaga gatcaaatta agccagtagc
      481 ggccgcgtaa caggcaacct gggtcttatt tggtgcatta atttttttct gcatgttttt
      541 ctggtggaaa ttgaccgtat tctcagagat tgacaaaatc atcgctatct ctgctgatgt
      601 cttcccttcc gccgtccacc tcagaatttc tttttcgcgc ttgctgaaat tcatctctgg
      661 cgtcatcact atttcatcat ttaaacgcat cagagccatc agactttcgc gcaccagtaa
      721 ctgcattttt aattgcagtt catcactaag aatgggtatt tcgcgcgcgc tgcaacggga
      781 aaaggacaaa aagcccagcg cccggtttgg cagcattaaa tactgagtga caccgcggcg
      841 taaaccatgt gcgcgcgcgg cttcccataa cggctgtgct tcgttgaata agtcatcatt
      901 ccacattaga tggccctgac taaagttttc agggttcagc accggatcaa ttgcgagaaa
      961 gttttttgcc tgataataac taacccacgc ctcagggtaa ttggtgtaga aagccacttt
     1021 aggtcgagtg aatggtaccg ggtggcggac acataacgaa tagtaatcgt actccagctg
     1081 ctgagcctga agctcaattt catggtagac ctcttctgcg gtctccatcc tctgaaaacg
     1141 caacagcatc gtgcgacgcc agctgaaaaa atccgtatcc tgcatagtaa accgcaacgc
     1201 ccctgagagt gagtatcatt tataatgata attcaaacta tcacataaat aagatttata
     1261 tataatttat attattcagg caatgaatta cttttgcaag ccatcgcatt ctcttatgtt
     1321 attaatgagt tatgctgatt ttttaagcag ttttatcagg cttgaaatgg cgttccgccc
     1381 cgactgtcga atcgtcgggg ctgatttttt cttattattg cagcagaaac ttctcgagga
     1441 actggcgggt gcgaggctgc tcgggatcgg caaataacgc ttttgcggcc ccctgctcga
     1501 ctatccgccc ctggtccata aaaatcgccc ggtccgcaac atcccgggca aagctcattt
     1561 cgtgtgtcac aatcaccatc gtgcgctttt cctgcgccag ctgacggatg gtgttcagga
     1621 cttcgcccac cagctctgga tccagcgctg acgttggctc gtcaaacaaa atcacctcag
     1681 gacgcattgc cagcgcacgc gcaatcgcaa cacgctgctg ttgaccgcca gacaaacgac
     1741 gtggatagct ggtttcttta ccggccagcc caactttcgc caacagcttg cgagcgcgcg
     1801 ccgtggcctc ttctttcggt tcgcctttga cgatcaccgg cccttcaata atgttctcca
     1861 gcaccgtacg atgcggaaac aaattaaagt tctggaagac aaacccgacg tgctgacgca
     1921 actggcgaat cagcgatttt tgctgactta atgaacgtgc agtatcaata gtgatatcgc
     1981 caacggtgat cgttcccgct tccggttgtt ccagcagatt tatgctacgt agcaatgtgg
     2041 ttttgccgga accactcgga ccgataattg ccaccacttc gccaggcttt acctcgaggt
     2101 cgataccgtg cagcaccgtc tgaccgtgga attttttcac taggttctta acttcaatgg
     2161 cactcatttg ggttctctct cctggcgatt aagttgattc tcaaaatggt tctgcaacgt
     2221 cgataacaca gtcgccatga tccagtagat gagcgaagcc gccagataca tggtgaatac
     2281 ctccagcgta cgcgaggtaa tcaactgcgc ctgacggaac agctccggca cctggattgt
     2341 cgcggccagc gacgtatctt ttaccagact gataaagctg ttcgagagcg gcggcaacgc
     2401 cacccgcgcc gcctgtggca aaatcgcgcg acgcatagtc tgccatggcg tcataccaat
     2461 actggcagct gcttcccact gacctttatc aatagaagaa atggcggcgc gaagcgtttc
     2521 ggcagtatag gcggcagtgt tgagcgacag accaatcatt gctgacggaa tgggatctaa
     2581 ttcgatacca aactgcggca ggccgtaata gatcataaac agttgtgcga tgagcggcgt
     2641 accacgaaaa atagagatat aaaaacgcgc cagccagcgc acgggccaga ttggcgacag
     2701 gcgcatcagc gcgagaataa accctagcag taaaccaaaa aacatgccgc cgatactgag
     2761 ttgcagcgta tacccagccc ctttcagcag gaacggcaag gaatcaataa ccagttgtat
     2821 actttcttgc atgagcgttc ccggagctaa acgtggggat gataggcgaa cagcgcaggc
     2881 gcgccgccgg tatgaataaa cagaatcggc ccttcatctt tgaagcgttt ctgactgata
     2941 ccgtcaatca gacccgccat cgcttttccg gtatacacag gatcaagcag aatgccttcc
     3001 agccgcgcca gcaatttcac tgcttccatg ccttcgtcgt tcggcacgcc gtagccaggt
     3061 gcaaaatagt catcccagag taaaatttcc actgatgcgg tcagctccag ttctttcgca
     3121 atcgcctgtt gtaggttaac cactttcggc aattgatcgg caacggaacg cgacacggtc
     3181 acgccaatca gttcgctttc aggcatcagg tgttccagcc caacagccag tccggcgtga
     3241 gttccggcac tgcccgatgc gactaccacc gatgaaatat taaccgcccc ttcacactgt
     3301 tgcgcgattt ccagcgcact ctccacataa cctagcgcgc caagagcatt agaaccgcca
     3361 accggaatga catacgggcg aaagccttgt gcttcgactc gcgtcgccag ctcttccagt
     3421 tgggcattgg gatcggtcag tgcgtcgcac atttcaatct gggtattgaa cagatccagc
     3481 aacaagcgat tgccgttggt taaatagttt tctgcggttg tgccaatagg attttccagc
     3541 agcgccacgc agtgcagacc gagtttggca gcgactgcgg cagtctgccg cacatggtta
     3601 gactggatcg ccccggcagt aatcagcgta tcggcacctt cacgcagagc atctgccgcg
     3661 agaaattcta gcttacgtaa tttattgccg cccattgcca tgggggtgac gtcatcccgt
     3721 ttgatgaaaa tttcccgtcc tagataatca gaaaagcgcg gcagatattc gagcggcgtt
     3781 ggcgcgccga taaactccag ccgtggaaaa cgggttaaat tatgcagtgg cataacagcc
     3841 tccgatgtgt gttgttgtga ttttctcatt atgcacgctg aaaacgcgta aataaaaaag
     3901 gcgctagtga aagcgccctt ttttgtcatt atgctgatta tttggtcaca tcagcaccaa
     3961 accatttttc ggaaagggct tgcaaagtgc cgtctttttg catttccgca attgcatcat
     4021 tcactgcttt cagcaggtct tcatttcctt tacgcagcgc cacgccagac tcctgacggg
     4081 agaatgcttc accggttact gccagcgtat cgttggtttt cttcaccaga tccagcgccg
     4141 ccagacgatc aacgaggatc gcatcgatac gccctacgcg cagatcctga tatttggtcg
     4201 ggtcatcatc ataggtacgc acatcgacgc cctgaacatt ctgccgcagc cactcttcat
     4261 agttggtgcc cagaccgacc cccacttttt tgcctttcag atcatcggct gttttaatgg
     4321 tgccttcgtt accttttttc accagcgcct gaataccaga aatggtgtac ggggttgaga
     4381 aatcgtattt tttcttgcgc tcatcagaaa tggtgacctg attaatcacc acatcaatac
     4441 gtttagagtc cagcgacgcc agcataccgt cccatttggt cggttttagt gacgcctcaa
     4501 cgccaagatg ttttgccagc tgttgggcaa attccacttc aaaaccggtt aatttgccgt
     4561 catccccctg aaaactgaac ggcggataag taccttccag ccctaccagc agcgtgccgc
     4621 gctctttaac tttattaagc agaccttcat ctgcaaaact tttaacgctc atgcccgcaa
     4681 tcagcgctac ggccatcaca cccatcaatg cctgacgtcc cagatgtgct aatttcatat
     4741 tcaccccgaa tgttgttatg tctgtttgca gtgtagagcc attgtttgta aacacaaaaa
     4801 caactccgct acatcttatt cttatttaat atatatcaga agcaggcagg ctggaggatt
     4861 tctgcacaag tccggtacag gtttggcgct gatagtgttg atacttccgt aacgcgatgc
     4921 ggtaattgtt ggtgctggtg gtgggcagcc acggggcgag gatttcatca aggaaaccgt
     4981 cctgcagctg atcgagggaa atattttgct cgtgtagatg attccccaaa cggcgcagac
     5041 gaacgacata ttcacgcacc gtaccagggc tcatttcagt ttgctcaaaa agaaattgct
     5101 taaagccgat aatatcgaaa aaatcactct gcgttttgca atgtaaatca ccgcaaaatc
     5161 ggcacaatgc cgcccatgat ttttgttccg tttgccagcc attttcatca agcagcgtgt
     5221 ccaggcggga aatctcgatt ttattcacta ttttgccgtc gcgaactaag gtaatgcgat
     5281 cgagcaattt acggcaatgc gcgcaatggg tctggctgtg tttaaagtct ttaagatagc
     5341 ggcttaatgg ccgtcttttc aggtgctgca ccatcattaa gaactcctgg tagtcaaagt
     5401 taaagtgcgg catttactga cgttataact tacccagttt agtgcgtaac cgtttaatag
     5461 cctggctgtg taactgactg acccgcgatt ccccgacctc cagcactgca ccaatctctt
     5521 tgagattcag ctcttcctgg taatagaggg ttaataccag tttttcgcgc tccggcaacg
     5581 tttcgatagc ttccatcacc cgctggcgca gattactgtc cagtagttgt tgtagcgggt
     5641 tttctcgttg atgatcatca gtaaccaatt cgatgctatc gccgtgctct tcgcgccact
     5701 catcgtagga gaagagctgg ctgttattgg tgtcgagcaa catttggcga taatcggcaa
     5761 tatcgatccc taaacgttcc gctacctcag tttccgtggc gttgcggcca agttcctgct
     5821 ccagttgccc tattgcctgt gccacttcac gcgcgttgcg tcgcacgctg cgcggcaccc
     5881 agtcacggct gcgaagttca tccagcatag cgccacggat acgctgcact gcgtaagttg
     5941 taaatgccgt tccttgtagg gcgtcatagc gttcgacggc attaagtaac ccaatgccgc
     6001 ccgcctgtag cagatcgtca agttccacgc tcgcgggcag tcgaacctgc aggcgcaatg
     6061 cttcgtgacg caccagcggg acataacgct gccacagcga gtgtttatcc attacacctt
     6121 cagcggtata gagtgaattc acgataaaca gccctgcgtt atatgagtta tcggcatgat
     6181 tatccgtttc tgcagggttt ttaatcggac gattagtggg tgaaatgagg ggttatttgg
     6241 gggttacagg taaattccag gcagaaaaaa ccccgccggt ggcggggaag cacgttgctg
     6301 acaaattgcg ctttatgttg ccggatgcgg cgtaaacgcc ttatccggcc tacaagaatg
     6361 tgcaaattca ataaattgca attcaacttg taggcctgat aagcgcagcg catcaggcaa
     6421 tttggcgttg ccgtcagtct cagttaatca ggttacaacg attaaccctg cagcagagac
     6481 agaacctgct gcggtacctg gttagctttt gccagcacgg agttaccggc ctgctgaatg
     6541 atctgcgctt tcgacatgtt ggacacttcg gtcgcatagt cggcgtcctg aatacgggac
     6601 tgcgcttcgg acaggttggt agtggtgttg ttcaggttgg tgactgcgga atccagacgg
     6661 ttctggatag cacccaggga agaacggaat ttgtcgatgg agctgattgc gtcgtccagg
     6721 gcagcaagcg ggttggtcgt tgcagaacca gcactggtag tctcagtagt gattttaccc
     6781 gcggagttca catttacagc agtaccaatt gctggagcat aatctttatt ggtatcagtc
     6841 gctgaagcat acccggcaac agtcacagag ccgttatcct tgttaacgct gtaagagact
     6901 gtatagtcgg caacgttagt gataccacct ttatcatcca cataagactt cgcagcatca
     6961 ctggattcac ccgcggtaaa cccaatagtt ttgctcagta caccggaatt aaaggtaatt
     7021 gaagatgtcg cggcagccgc tttggtttcg ctcaatacta catctttact cactgtagcc
     7081 tgataagtaa tcccaccagg gattaacgga gctactggag tggttgtcgc aggagttgct
     7141 tttgcgatgg tatattctgt gccattgaat gtcagagagg caccgtcact accttcgctc
     7201 gctgctttga gcagcgcttt catatcagct ttagctgcgc taccagcgtt gttagtcgtc
     7261 aagttgcctg catcgtctac gtatgcctgg cttccaccga tggtgatatt acctgctgaa
     7321 tccgtttcga aagaaacagt accatctttt gtggtgtaag aaccattaac agatttaccc
     7381 acatcagcat gaagggtggc agctaacgca tcacgttttg aagctgaatc agcagcctgc
     7441 gccgcggcgt ctacacccgt accagcagta gatttagtgg tagtaaaatc accagattta
     7501 gcgttgtaag tataatctac gccgccaact gtgactttat cgccattccc taatttatcg
     7561 aatgcagcat cggtagttaa caaggtattt tcggttttca gatcataaag acctgtcgtg
     7621 gtgtttaact tcgcgccagc agaagttaaa tcacttaccg ttgcagcttt gttggtaata
     7681 gtacctttac catttacgtt aaagccattc agacccagag tatcagaatc gatttttttc
     7741 aggtcgatcg tgatggtttc gccgtcattc gcaccaacct gaattttcat tgaaccgtct
     7801 ttcgccagca cgttcacgcc gttgaactgg gtctggccgg atacgcggtc aatttcatca
     7861 agacgagatt tgatttcgtc ctggatggag tccaggtcag aatcggagtt agtccctgta
     7921 gtggcctgaa ccgtcagttc acgaatacgc tgtaagttgt tgttgatttc ggacagcgcg
     7981 ccttcggtgg tctgcgcaac agaaataccg tcgttggcgt tacgggccgc ctgagtcagg
     8041 cctttaatgt tagaagtaaa acggttagca atcgcctgac ctgcggcgtc atccttcgcg
     8101 ctgttaatac gcaagccaga agacagacgc tcgatagaac tcgacagcgc agactggttc
     8161 ttgttgatat tattttgagt gatcagcgag aggctgttgg tattaatgac ttgtgccatg
     8221 attcgttatc ctatattgca agtcgttgat tacgtattgg gtttccaccc gtcggctcaa
     8281 tcgccgtcaa acctgttatc gtctgtcgta aaacaacctt tagaattttt ttcacaaaca
     8341 gccatttttt gttagtcgcc gaaatactct tttctctgcc ccttattccc gctattaaaa
     8401 aaaacaatta aacgtaaact ttgcgcaatt caggccgata accccggtat tcgttttacg
     8461 tgtcgaatga taaaaggaaa tcgcatggca agtatttcat cgctgggagt cgggtcaggt
     8521 ctggatttaa gttcaatcct tgatagcctc accgccgcgc aaaaagcgac gctaaccccc
     8581 atttcaaatc agcaatcgtc gtttaccgct aaacttagcg cctacggtac gctgaaaagc
     8641 gcgctgacga ctttccagac cgccaatact gcattgtcta aagccgatct tttttccgcc
     8701 accagcagca ccaccgcgtt cagtgccacc accgcgggta acgccatcgc cgggaaatac
     8761 accatcagcg tcacccatct ggcgcaggcg caaaccctga ccacgcgcac caccagagac
     8821 gatacgaaaa cggcgatcgc caccagcgac agtaaactca ccattcaaca aggcggcgac
     8881 aaagatccga ttaccattga tatcagcgcg gctaactcgt cgttaagcgg gatccgtgat
     8941 gccatcaaca acgcaaaagc aggtgttagc gcgagcatta ttaacgtggg taacggtgat
     9001 tatcgtctgt cagtcacatc aaatgacacc ggccttgaca atgcgatgac gctctcggtt
     9061 agcggtgatg atgcgctaca aagttttatg ggctatgacg ccagtgccag cagcaacggc
     9121 atggaggtct cggtcgccgc ccagaatgcg cagctgacgg tcaacaacgt cgccattgag
     9181 aacagcagca acaccatcag cgacgcgctg gaaaacatca ccctgaacct gaacgatgtc
     9241 accacgggca accagacgct aaccatcact caggacacct ccaaagcgca aacggcgatt
     9301 aaagattggg tgaatgccta taactcgcta atagatacct tcagcagcct gaccaaatac
     9361 accgccgtag atgcgggagc tgatagccag agttctagca atggcgcact gctcggcgac
     9421 tccacgctgc ggacgattca gacgcagttg aagtcgatgc tgagtaatac cgtcagttct
     9481 tccagctata aaacgttggc gcagattggt atcacgaccg atcccagcga tggcaaactg
     9541 gaactggatg ccgacaaact caccgctgca ctgaaaaaag atgccagcgg cgtaggtgca
     9601 ttgattgttg gcgatggtaa aaaaaccggc atcacgacca ccatcggcag caacctgacc
     9661 agttggcttt cgacaacggg cattattaaa gccgctaccg atggcgttag taagaccctg
     9721 aataaattaa ctaaagacta caacgccgcc agcgatcgca ttgacgcgca ggtcgcgcgc
     9781 tacaaagaac aatttaccca actggacgtt ttaatgacct cgttaaacag cacaagcagc
     9841 tacttaacgc agcagttcga aaacaacagt aattccaagt aatcattatt catcgggaga
     9901 caggtcatgt acgcggcaaa aggcacccaa gcctatgcac aaattggcgt cgaaagcgcc
     9961 gtaatgagcg ccagccagca gcagctggtc accatgctat ttgatggagt gctgagcgca
    10021 ctggttagag cgcgcctgtt tatgcaggac aacaatcagc aaggcaaagg cgtctctttg
    10081 tcaaaagcga tcaacatcat tgagaacgga ctgcgggtga gtcttgatga agagagcaaa
    10141 gacgaactaa cccaaaactt gattgctctt tatagctata tggtcaggcg tttgctgcaa
    10201 gccaatttac gcaacgatgt ctccgcagtc gaagaagtgg aaacattaat gcgcaatatt
    10261 gccgatgcct ggaaagagtc gttactctcc ccttctttga ttcaggaccc agtctgatga
    10321 accatgcacc gcatttatat ttcgcctggc aacaactcgt cgaaaaaagc cagctcatgt
    10381 tacgcctggc aacggaagaa caatgggacg aactcatcgc cagcgaaatg gcgtatgtta
    10441 atgcggtgca ggagattgca catttgactg aagaggttga cccgtccacc acgatgcagg
    10501 agcagctccg cccgatgctg cgcctgattc tcgacaacga aagcaaggta aagcagttat
    10561 tacagattcg gatggatgaa ctggcgaaac tggtcggtca gtcatcggtg caaaaatcgg
    10621 tgttgagtgc ctatggcgat cagggcggct ttgtgctggc tccgcaggat aacctctttt
    10681 gaatctgaat gagtcgatgg ctcgcgaata atccgattac ggctacgctt ctaatgttcc
    10741 ccttgaatgg agtcgaagaa tgcgtaatcc cacgctgtta caatgttttc actggtatta
    10801 cccggaaggc ggtaagctct ggcctgaatt ggccgagcgc gccgacggtt ttaatgatat
    10861 tggtatcaat atggtctggt tgccgcccgc ctataaaggc gcatcgggcg ggtattcggt
    10921 cggctacgac tcctatgatt tatttgattt aggtgagttt gatcagaaag gcagcatccc
    10981 tactaaatat ggcgataaag tacaactgct ggccgccatt gacgccctga aacgtaatga
    11041 cattgcggtg ctgttggacg tggtagtcaa ccacaaaatg ggcgcggatg aaaaagaagc
    11101 tattcgcgtg cagcgcgtta atgctgatga ccgtacgcaa attgatgaag aaatcattga
    11161 atgcgaaggc tggacgcgtt acaccttccc cgctcgtgcc gggcaatact cacagtttat
    11221 ctgggatttc aaatgtttta gcggtatcga ccatatcgaa aaccccaatg aagatggcat
    11281 ttttaaaatt gttaacgact acaccggcga aggctggaac gatcaggttg atgatgaact
    11341 cggtaatttc gattatctga tgggcgagaa tatcgatttt cgcaatcatg ccgtgacgga
    11401 agagattaaa tactgggcgc gctgggtgat ggaacaaacg caatgcgacg gttttcgtct
    11461 tgatgcggtc aaacatattc cagcctggtt ttataaagag tggatcgaac acgtacagga
    11521 agttgcgcca aagccgctgt ttattgtggc ggagtactgg tcgcatgaag ttgataagct
    11581 gcaaacgtat attgatcagg tggaaggcaa aaccatgctg tttgatgcgc cgctgcagat
    11641 gaaattccat gaagcatcgc gcatgggacg caactacgac atgacgcaga ttttcactgg
    11701 gacgctggtg gaagccgatc cttttcacgc cgtaaccctc gttgccaacc acgacaccca
    11761 accgttgcaa gccctcgaag caccggtcga accgtggttt aaaccgctgg cgtatgctct
    11821 aattttgttg cgggaaaatg gcgttccttc ggtgttctat ccggacctct acggtgcgca
    11881 ttacgaagat gtcggtggtg acgggcaaac ctatccgata gatatgccaa taatcgaaca
    11941 gcttgatgag ttaattctcg cccgtcagcg tttcgcccac ggtgtacaga cgttattttt
    12001 cgaccatccg aactgcattg cctttagccg cagtggcacc gacgaatatc ccggctgcgt
    12061 ggtggtcatg tcgaacggag atgatggcga aaaaaccatt catttaggag agaattacgg
    12121 caataaaacc tggcgtgatt ttcttgggaa ccggcaagag agtgtagtaa ccgacgaaaa
    12181 cggcgaagcg accttctttt gcaacggcgg cagcgttagc gtgtgggtta tcgaagaggt
    12241 gctttaaatt catccccggc ggcaagccgg ggagatttca ttacggcaat ggcgtcggta
    12301 acggcgcttt atccagcgca gcggcacact ctgccgtcgg gcgatccaca cgctccatcg
    12361 tcataccatc gtactcaatg gtgttaccgt cacgctcaac ctcgtacagc tcacgtttta
    12421 cggtaacgtt agtgagatca tcagacatca atgtcaactt gcccggcaca gcaatcaccc
    12481 gctgccactg acggcaatct agggtatcgc cctctttagt gacaatcagg ctgcctatcg
    12541 cttccgggct gaccagcgca cgttgcggcc ctttggtctg ccagtagccc gccagccagt
    12601 ccggcgcggg ggttttcacg acattgttat agttttcgac ttcggcacag cccgccagca
    12661 acatgagcgc gcctgcaatt gcgagttttt tcatcattcg tcctgcatga gaagaaaaga
    12721 ggattgtggc attaaagccc tgatgtcgcc agcctttcca ggttcagaga gaaatttgat
    12781 ctaactatta tttttgtgaa ttattacccc gaaaataaat ttgcatggtg tatgattcgc
    12841 gactcttgtt tcaaaccttc tgagttcaga ggctacattc atgtcatggc agcaattcaa
    12901 acacgcctgg ttgattaaat tctgggcgcc catccccgcg gtcatcgcgg cgggtattct
    12961 ctccacttac tattttggca tcactggcac cttttgggct gtcacgggtg aatttacccg
    13021 ttggggcggt cagctcctgc agctgttcgg cgtccatgct gaagagtggg gttattttaa
    13081 aattatccat ctggaaggat cgccattaac ccgcatcgac gggatgatga tcctcggtat
    13141 gtttggcggc tgctttgccg cagcgctgtg ggccaacaat gtcaaactac gcatgccgcg
    13201 tagccgtatc cgcattatgc aggccattat tggcggcatt atcgccggtt ttggcgcgcg
    13261 tctggcaatg ggctgtaacc tggcggcatt ctttaccggt attcctcagt tctcgctgca
    13321 tgcctggttc tttgccatcg ccactgccat tggttcatgg tttggcgcgc gctttaccct
    13381 gctgcccatc ttccgtattc ccgtgaaaat gcagaaagtt gctgccgcct caccgctgac
    13441 gcaaaaaccg gatcaggcgc ggcgtcgttt tcgtctcggg atgctggtct ttttcggcat
    13501 gctgggctgg gcgctgctca cagcgatgaa ccaacccaaa ctggggctgg caatgctgtt
    13561 tggcgtcggc tttggtttac tgattgaacg tgcgcaaatc tgctttactt cggcgttccg
    13621 cgatatgtgg atcaccggac gtacccatat ggcgaaagca atcattattg gtatggcggt
    13681 gagtgccatc gggatcttca gttacgtaca gttaggcgtt gaacccaaaa tcatgtgggc
    13741 gggaccaaac gcggtaattg gtggtttact gtttggtttt ggcatcgtgc tggctggcgg
    13801 ctgcgaaacc ggctggatgt accgcgcggt agaaggccag gtgcactact ggtgggtcgg
    13861 tctgggcaat gtgatcggct caacgattct ggcgtattac tgggatgatt tcgctccggc
    13921 gctggccacc gactgggaca aaatcaactt gctgaaaacc tttggtccga tggggggcct
    13981 gctggtgaca tatttgctgt tgtttgctgc gctgatgttg attatcggct gggaaaaacg
    14041 cttcttccgc cgtgcggcac cgcagactgc taaggagatc gcatgaaaaa tatcgttcct
    14101 gattaccgcc ttgatatggt gggtgaaccc tgcccttatc cggcagtcgc aacccttgag
    14161 gcgatgccgc agttaaaaaa aggggaaatc ctggaagtgg tgagcgactg tccgcagtcg
    14221 atcaataata ttccactgga tgcgcgtaat cacggctata cggtgctgga tattcagcaa
    14281 gacgggccga ccattcgtta tttaattcag aagtaatccc tcattcccgc tgtgacccac
    14341 agcgggaaca tcgttacctt tgtatcacat agattttacc cgccccctct cctattcttt
    14401 tcagtatgat ctcaggaggt ttctatgtgt ggacgctttg cccaatccca aacgcgtgaa
    14461 gattaccttg cgcttctcgc ggaagatatt gaacgcgata ttccctatga tcccgaaccc
    14521 attggcagat acaacgtcgc gccgggaacc aaagtcctac tgctcagtga acgtgatgaa
    14581 caccttcatc tggatccggt tttctgggga tatgctcccg gatggtggga taaaccgccg
    14641 ctgattaacg cccgcgtaga aactgcggca accagtcgta tgtttaaacc gctctggcaa
    14701 catggtcggg caatctgttt tgccgatggc tggtttgagt ggaaaaaaga aggcgacaaa
    14761 aagcagcctt attttatcta tcgtgctgat ggacaacctg tttttatggc agcgataggc
    14821 agcacaccgt tcgagcgcgg tgacgaagcc gaaggatttt tgattgtcac tgcagcagca
    14881 gaccagggtc tggtagatat tcatgaccgc cgcccgctgg ttctgtcgcc agaagctgcg
    14941 cgggaatgga tgcggcaaga gattagcggt aaagaagcct cagaaatcgc ggccagtggc
    15001 tgtgttccag caaaccagtt ctcctggcac ccggtatcgc gcgcggtggg taatgttaaa
    15061 aaccagggtg cggagttaat tcaacctgtt tgataagcct ggagattatt gatcaacaat
    15121 actgcgtcat aagtaatctc tattagacaa agatttcatt acctgttggc atattgcaaa
    15181 aataacacca atacggaatc gtcatgttca cgattaaaac agatgatctc acccatccag
    15241 cagtgcaagc attagtggct taccatattt ccggcatgct gcagcagtct ccccctgaaa
    15301 gcagtcatgc tttagacgtg caaaaattac gtaacccgac agtgacattc tggtcagtat
    15361 gggaaggcga acaactcgca ggaattggtg cgctgaagtt gctggatgat aaacatggcg
    15421 aactgaaatc aatgcggacc gcgccaaatt atttacgtcg gggtgtcgcc agtctgattt
    15481 tacgccacat tttgcaggtc gcccatgaca gatgccttca tcgcctgagt ttagaaacgg
    15541 gtacacaggc tggatttacg gcctgccatc aactttattt gaagcatggt ttcgttgatt
    15601 gcgaaccgtt tgccgattat caacttgatc cacacagtcg atttttgtca ttgacgctat
    15661 gcgaagataa tgagttgctt tgagccagac gcagcacatt cttgcattcg acgtgctgcg
    15721 tctttattta tcaccaacag gaaacgcctt gtccatagac gcccctt
//
LOCUS       DBFIFM010000107        15748 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.107, whole genome shotgun sequence.
ACCESSION   DBFIFM010000107 DBFIFM010000000
VERSION     DBFIFM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.107"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..183)
                     /locus_tag="N7C74_002867"
     CDS             complement(<1..183)
                     /locus_tag="N7C74_002867"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432701.1"
                     /translation="MIDLAPFAALTMSSEKGLKKFIFTNTFFATTRSGAFCYNLKSNI
                     IISGNYSCVPHHLRKLQ"
     gene            complement(335..928)
                     /gene="tehB"
                     /locus_tag="N7C74_002868"
     CDS             complement(335..928)
                     /gene="tehB"
                     /locus_tag="N7C74_002868"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HGU2432702.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGEYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(925..1917)
                     /gene="tehA"
                     /locus_tag="N7C74_002869"
     CDS             complement(925..1917)
                     /gene="tehA"
                     /locus_tag="N7C74_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HGU2432703.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTLALLMQGKLLVRTERAVLMKAEDKE"
     gene            2041..3021
                     /gene="ydcK"
                     /locus_tag="N7C74_002870"
     CDS             2041..3021
                     /gene="ydcK"
                     /locus_tag="N7C74_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HGU2432704.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     INQGAYISDSVTIHDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(3016..3552)
                     /gene="rimL"
                     /locus_tag="N7C74_002871"
                     /pseudo
     CDS             complement(3016..3552)
                     /gene="rimL"
                     /locus_tag="N7C74_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
     gene            complement(3615..3839)
                     /gene="ydcH"
                     /locus_tag="N7C74_002872"
     CDS             complement(3615..3839)
                     /gene="ydcH"
                     /locus_tag="N7C74_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HGU2432705.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDDRGY
                     NAEVVRMKKQKLQLKDEMLKILQHESVKEV"
     gene            complement(3979..5634)
                     /gene="opgD"
                     /locus_tag="N7C74_002873"
                     /gene_synonym="mdoD"
     CDS             complement(3979..5634)
                     /gene="opgD"
                     /locus_tag="N7C74_002873"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HGU2432706.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(5859..7202)
                     /gene="ydcJ"
                     /locus_tag="N7C74_002874"
     CDS             complement(5859..7202)
                     /gene="ydcJ"
                     /locus_tag="N7C74_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU2432707.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYDQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            7419..8342
                     /gene="ydcI"
                     /locus_tag="N7C74_002875"
     CDS             7419..8342
                     /gene="ydcI"
                     /locus_tag="N7C74_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU2432708.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGPNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(8380..10020)
                     /gene="trg"
                     /locus_tag="N7C74_002876"
     CDS             complement(8380..10020)
                     /gene="trg"
                     /locus_tag="N7C74_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HGU2432709.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRTLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHYA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKQSVLAEPRGAGEPVSFAPV"
     gene            10419..10568
                     /gene="hokB"
                     /locus_tag="N7C74_002877"
     CDS             10419..10568
                     /gene="hokB"
                     /locus_tag="N7C74_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HGU2432710.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDSDKEVAALM
                     ACTSR"
     gene            complement(10546..10614)
                     /gene="yncP"
                     /locus_tag="N7C74_002878"
     CDS             complement(10546..10614)
                     /gene="yncP"
                     /locus_tag="N7C74_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HGU2432711.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(10640..10813)
                     /locus_tag="N7C74_002879"
     CDS             complement(10640..10813)
                     /locus_tag="N7C74_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432712.1"
                     /translation="MKKLALILFMGALVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(11058..11588)
                     /gene="cybB"
                     /locus_tag="N7C74_002880"
     CDS             complement(11058..11588)
                     /gene="cybB"
                     /locus_tag="N7C74_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HGU2432713.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            11777..12778
                     /gene="gap"
                     /locus_tag="N7C74_002881"
     CDS             11777..12778
                     /gene="gap"
                     /locus_tag="N7C74_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGU2432714.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYKVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(12820..14259)
                     /gene="aldA"
                     /locus_tag="N7C74_002882"
     CDS             complement(12820..14259)
                     /gene="aldA"
                     /locus_tag="N7C74_002882"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HGU2432715.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(14456..15256)
                     /gene="ydcF"
                     /locus_tag="N7C74_002883"
     CDS             complement(14456..15256)
                     /gene="ydcF"
                     /locus_tag="N7C74_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HGU2432716.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDVPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(15372..>15748)
                     /locus_tag="N7C74_002884"
     CDS             complement(15372..>15748)
                     /locus_tag="N7C74_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432717.1"
                     /translation="DERFINITPTSLSFKHADLSGLSYKNARLELALICPAIKSEVKI
                     SFDWIHSFRVTDEGDLLGMSGKQGINQTGIYRVANSTYLTWFTMQSCEIHKNKMIEHY
                     VIATSNDVIDVLSTVSPQIVYD"
BASE COUNT         4099 a         3961 c         3654 g         4034 t
ORIGIN      
        1 ctgcaacttt cgcaaatgat gtggtacgca tgaatagttt cctgagatga ttatattact
       61 ttttaagtta tagcaaaaag cgccgcttcg tgttgtggca aaaaaagtgt tggtgaaaat
      121 aaattttttt agcccctttt cagacgacat agtcaatgcc gcaaaagggg ctaagtcaat
      181 catcagtagt taaatttcag taagttaagc tgctaaaaaa cttcgactac tgtgattaat
      241 ttcacatccc ttcgataaaa aatatgctta ccgaaaagaa ggacagtaaa ccatctcagc
      301 gtggggaatt ttcacgcgct ttgttcttac cgggtcattt tttacgtgcc agcatcgtgg
      361 cgaaacgcag tttaatacga ttaccgttgg cgtcggtgcg gtgcagctcg ccgacgtctt
      421 cattgtattt caccatctcc cagccttcgt aatatcgacg taattctccc tctttgaagg
      481 caaacgggaa gccgacggta catggataat cagcggtatc catcgccgcc acaatcaggt
      541 tgtaaccgcc aggtttagtg caacgttgca tattggcaat cagccctggg atggttttag
      601 cctcaaggaa catcagcacc acagtcgaaa gaataaaatc gtactctcca tcaaatgtga
      661 ggttattcag atcaacgacc cgggtgtgta aattatccag attttcaatg gatttaatgc
      721 gctcgacgtt ggcgatactc atggcatttt tatcccatgc gtcaacatca taaccattgg
      781 ctgccaggta aagactgtta cgaccattgc cacagcccag atccagcgtt ttacccggtt
      841 taaccacttt caccgcttcc agtacttcag agtgtgtgcg ggttaattca tatttatcag
      901 taaaataatt ttcgtcacga atgatcattc tttgtcctct gctttcatta aaacggcgcg
      961 ctcggttctg actaacaatt ttccctgcat cagaagcgcc aaagtacgga tgagcagtat
     1021 tgcaataata aaattggtaa agataaacag cggcaccgcc agcgtatgga aaaatccatt
     1081 atcgctgcca ctccccagat gcaaaccggt ggttgccagt gcagatacgc cgaacgagaa
     1141 actccagaat gaagcattaa atggctggga gagataccat ggcatcagac gtagcataaa
     1201 cagcagttgc agcagtccat aaccgaaaag cattttcgcc agcgtgtcac cctcgccgcc
     1261 gttgacgctc agccaggcac tacaggccac cagcgcagga gcgagctgaa tgccgagtga
     1321 tgtccgcagt gccgtgggta attctcccga actacgcaga cgctgcaaga tcaccggttc
     1381 aaggcttagc catgagaaaa cgcctgcgcc taaaaacacc agaccggcgt cggtgtagcc
     1441 caacgcacca caggccattg cgctgataaa gttgttggca actgtcggca gatacagtcc
     1501 aggcgtagta gcttcttcag ggtgagatcc gcgccataat cccgcagttt gccaggcggc
     1561 ataagccaac tgaaccacga caccaaaact gaacaggcac accgccagtg ggcgaaacca
     1621 cggaacaaaa ccaatcgcca ccagcatcgt cgttgcagga aacaaactca caaaactgct
     1681 cagcactgga tggcgaactt ccgccagcac gctatgcgga aagcgtatga gtcgggtaat
     1741 aaatgcgcta gtcaataatc cccagatgat catcgccaga atcaccagcc catcccctaa
     1801 ccagtggctg acctgccaaa cctggctggc atagcgccag gcaaatccca tccctatcgt
     1861 ccccaacaca ataccaaagt agcctgccgg caaattgagc actttatcgc tctgcatctt
     1921 cttactcatt ttgtttaatt tataaagtat atttgaaatg cattttatgg aattttctcg
     1981 agcatagcca gagccgcaga atttgctacg gttatagtaa gtcacctgtg ggaaaacgct
     2041 atgcgcaaat atcgtctaag tgaagaacag cgagccttta gttatcagga agatggcact
     2101 aaaaaaaatg tgttattacg gcagattatc gccattagcg attttaacga tgtgatagca
     2161 ggaaccgctg gcggctggat cgatcgcgaa acggtgttag cacaggaagg aaattgctgg
     2221 atttacgacc agaatgctat tgcgttcggc ggaacggtga tttccggtaa tacgcgcatc
     2281 accggcacca gcgtactgtg gggagaggtt tacgcaacgg ataatgtctg gatcgataat
     2341 agtgaaatca accagggtgc atatataagt gacagtgtca ccattcatga ctcactggtt
     2401 tgcgggcaat gtagaatatt tggtcacgcc ttaattgatc aacattctat gatcgtcgcc
     2461 gcacaaggct taacgcccga ccatcaactc ctgctccaaa tttatgatcg cgcaagggtt
     2521 agcgcctcgc gtattgtcca ccaggcacaa atttatggcg atgccgtcat ccggtatgct
     2581 tttattgaac atcgcgccga agtttttgat tttgccagta ttgaaggtaa tgaagagaac
     2641 aacgtctggc tatgcgattg cgctaaagtc tacggtcacg cgcaggtgaa agctggcata
     2701 gaagaagatg ccattccaac gattcattac agttcgcagg tggcggaata tgccattgtg
     2761 gaaggtaatt gtgtgttaaa acatcatgtc ttgattggcg gaaatgccgt ggtacgtggc
     2821 ggaccgattc tgctcgacga gcacgttgta atacaaggtg agagccgtat tacaggtgcg
     2881 gtgataattg aaaaccatgt ggagttaacc gaccacgccg tcgttgaagc ctttgatggc
     2941 gacacggttc atgtccgtgg tccaaaagtc attaacggcg aggagcgcat tacgcgaacg
     3001 ccgctggcag ggttattatg aatcgataat acgcgcgtat aagttcacat catcataggc
     3061 atcattcagg aactcagcct gtttcaggca accttcaagg ataaaaccat tgcgcaaagc
     3121 gacctggttg ctttgcggat tgtccacccg acatttgatc acgaagcgtc taagttcacc
     3181 agactgagcg taatgatgaa tcagtgcctg cagcgcctga gaaatgatcc cctgcccctg
     3241 atgagattcg tccagtcagt agccgatttc agcggtttta ttcagtggtt caatacgatt
     3301 aaacgagata acgccgataa gttcatcttc tttgaaaatc atgaacattt tggcatagcc
     3361 gcgttgatgc aacatcacat taccctgcac cgtttttcgc gtgtcctctt caatctgaac
     3421 gaactgcggc cagtttagcg actgctgtaa ccaggtttta tttttacaga ttaactgata
     3481 aagaggagtg acgtgatttt ctgcaacagc atgtaattca agtgattcgc ttacttttat
     3541 cgtttcagtc atcttatctc cagtcaatcc atgcagttgt taaaaaagcc gcctggctgg
     3601 cggcttagga aagtttacac ctctttgacg ctctcatgct gcaggatttt gagcatctca
     3661 tcttttaact gtagcttttg ttttttcatg cggaccactt ccgcattgta ccctcgatcg
     3721 tcggaacctt cctttctggc aatttcatga tcaagtttat tgtgtttatc gaacaaggac
     3781 ataaagcgag gattttcgtt tttcagacgg gatattaaat ctcgatattc tggaaacata
     3841 cgcactccct gttagtggct gacaaggtgt atctgaaata cacaccatca gattactcat
     3901 ttaacttagg gaaaaatgcg agcgggatcg tgtttaacca gacggcacct cccgaagaag
     3961 gtgccgaaga aaaaacgatt aactcatcac gcggtcgtca acatactgac gtttatccgg
     4021 cgctggcggg aaatactgat acagccatgt ttcactgata gcgtcgccct gacaacgcag
     4081 atacatccgc atatcgaccg gatcagtgga gtccgaagtc ggataccagt caaactgaat
     4141 acgataacca tcaatgggtt caatatagag aatttcgatt tgcttcgctt ccccactgga
     4201 aagcgtaatc accggctcaa tgccttttgg tgcggcagct ttcagatcac caccaacgaa
     4261 atcgacggca aaacgacgcg cccatttttc gggatagtgt tcacctggtg cccaaccttc
     4321 cgggaaaccg cccatgccgg tacgcgtcgc cataacgcgc gctaatgggc aatgaacagg
     4381 cggttgcgca ctccagtaca gacgatactg gaatgcaaac tcatcacccg cctttacagc
     4441 tttttctggc tgccagaagc agacaatgtt atccagcgtt tcgcccgttg ttgggatttc
     4501 catcaggccg atggtgccct taccccactt gttacgcggt tccacccaca gacttgggcg
     4561 tttgttatac cagcccataa tgtcctgata atgggagaaa tcacgatcca gttgcaataa
     4621 accaaaccct ttcgggttgt tgtcggtgta agcattgaac tgcaattttt gcggattatt
     4681 cagcggacgg caaatccact cgccgttgcc ccgccacatg gacagacgat cagagtcatg
     4741 aatttgcgga tgaatggtgt cgcacatccg acgttcatta gtaccgcagc tgaacatact
     4801 ggtcatcggc gcaatgccca gctgtttaat gtctttgcgc gcatacaggt gattttccac
     4861 atccataatc acctgacttt tctcacaatg gatagtgaac ttataggcac cagtaatgct
     4921 ggcgctatcg agcaacgcat aaacggtaaa ggtagttgcc cccggtttta ccgtatcaaa
     4981 ccagaaggcg gtaaagtcgg ggaactcttc tttactgtcg gtgtaagtgt caatcgccag
     5041 gccgcgggcc gacaaaccgt attgatatgt gtcatcaacg gcgcggaaat aactcgctcc
     5101 gaggaatgat actacatcac ggcgcgccag ttcgggggct ttaaacacgc gaaaaccggc
     5161 aaagccgaga tcgctttgcc cttctaattg tttggtatca acacctgcat cgttgtattt
     5221 gaacaactcc gggcgaaagt gaatttcacg cgccagatgt gttgctggat ctacagaaaa
     5281 catacgaacg cggcgacgga atcccattcc catatggaag aactgagcgt ccagttgacg
     5341 gttctcaacg ttatgccaga gcgatttttc ggcgtcgtat tgaatactgt tataagcctg
     5401 cggcgtcatt gtcgccagcg tgtcaggtaa cggacgaggc gcaccacgcc acgctgtttg
     5461 cgctaagtcg tgcgccattg actgtagaat ggagaagtca aaacgctggg tttgcccgtc
     5521 ggcaatatca gaatctgccg cgaatgccgc ctgagaaaaa agagaagcaa tgccgctggt
     5581 accgcacacg gcggccatag ccattgaacc tttaataaat cgtctacgat ccatacctga
     5641 aagtgagtcc ttctggtgtt gtgaatggtc ccgcgctggt cgttttcagc ggcaagaaag
     5701 tgcttattta tgaccgctca ctctagacaa tatttattaa taatccaatt gttgaactga
     5761 gagaatatgc ataaatcggc aattttttta tgtcgatggg atgagcctga ttcgtttgta
     5821 aaatcaggca aatttttgca aaggcggatg gtcagaattt aaagcaaacc acagcgacgt
     5881 ttactgcgtt cttccgcttc ctgataaagc tggaactcat ccaaaaccgg acaacccaac
     5941 gcctgctcaa atgcttcgcg actggcatta ccgtggctgc gtgcctgcgt ttcattaccc
     6001 agatttgact ggaagatccc cgccgcgcta acgggcaaga aatcttcata ggtaatgggt
     6061 tgcgccgcga cccaaccacg ttcaattaag ggctgtggat cgtctccggg atgaatcgcc
     6121 tgacgatgcg cctcacctga aggcgtcaga cggtaccgga accatgccag cccttgctga
     6181 cgcattaaaa actcactgtc aggaaaagtg cggaaggttt cctgtaaatg catttggtga
     6241 gtgagattat cctgcccggt tccagcgtta cgcagaagat catcatacag ttgtcgacct
     6301 ttcggcgtta atgccacgcc acgctgctca atttcaccaa agcgcgcagt atgcgtgccc
     6361 tgtttctgcc ccgcaaacaa caccgtctct tccagtgctt taaagctggt ctggcgtagt
     6421 aaaatcggta cctcgcggcg cggcggccct tcgatcagaa tttttggttc aattccgcat
     6481 tcaggcatca tcgactgcac ccggtcaata tccagcgtac gtggcgtcag atggttgata
     6541 tggcatccag gaaaacagac cacatcagca atcaaccgat gttcgttgtg caaggcgcga
     6601 taggtttctt catctaccgt tgctgactgg tgccaacgga acgtttccag ggcctcctgc
     6661 acaaactcct gtgcctgtgt ttcgttaaaa ccgccccgct ggtcatactc ctctaacagt
     6721 tgtcgacaac gtggggtgaa gatatcgcgc tgacgcagaa tctccgccgc tttctggcgc
     6781 aaaatttcgt tctcgataag ctcaaggcgg agtaaggagg taaaaacgcg gaagggatta
     6841 cgcgccagag aagcatcatc aatgggccga aatgctgtcg aatgtaccgg cacccctgcc
     6901 tgcgagagat cgtaatagct caccgggtac atccccataa tggcaaacat ccgccgaaga
     6961 gtagcaagct cttgtgcagt cccaacgcga atcgccccat gacgttcaac attcagtcgc
     7021 gccagctcgt ctgcatttac cattttttcg tgcagttgag gattgttttc cagcacagcc
     7081 agattcacat cagctaccag ttccagcagc gtgccatatt gcggaacttc ttgctggtac
     7141 atggctgaca ttgcctgcga aaactgttcc cgaatctcat ccgccgtgat gctgttcgcc
     7201 atagtgtctg cctccagtgg tcagtaatct ggagtgtagg taaccgcatt cactctggcg
     7261 ggaagaattt acaaactgtg atctcgccgc gaaaacatca atattatcca ttttgctgta
     7321 acataattgc tttaattgtt aataatattt tgcaatcaag ttatcataat caaacaactt
     7381 cacttgtcag cgacaccgct tcgtttttaa catcgcttat ggaaaaaaat agtctgttta
     7441 gtcagcgcat ccgtttgcgc caccttcata cattcgtagc tgtcgcacaa caaggaactt
     7501 tggggcgcgc ggctgaaacc cttaatttga gtcaacctgc gctctctaag acattaaatg
     7561 aactggagca gctgacgggc gctcgcttgt ttgagcgtgg tcgtcagggg gcgcaactta
     7621 ccttacccgg cgaacaattt ttaacgcatg cagtcagagt acttgacgcc atcaacactg
     7681 ccggacagtc gcttcatcgt aaagaaggtc ctaataatga tgtcgtcagg gttggtgcac
     7741 tacctactgc ggcactgggg atattacctt cggttatagg tcagtttcat cagcaacaaa
     7801 aagagacgac cttgcaagtt gcgacaatga gtaaccctat gattctggcg ggtttgaaaa
     7861 ccggggaaat cgatatcggc attggtcgga tgtcagatcc tgaactgatg accgggctta
     7921 attacgaact gctgtttctt gaatcgctga agctggttgt ccgccctaat catccgctac
     7981 ttcaggagaa cgtaacgcta agccgggtgc tggaatggcc ggtcgttgta tcaccagaag
     8041 gcactgcgcc acgccagcat tcagatgcat tagtacagag ccagggatgt aaaattcctt
     8101 cgggttgtat cgaaacgctg tctgcttcgc tatctcgtca acttacggtt gaatacgact
     8161 acgtgtggtt tgtcccttct ggcgcggtaa aagacgacct gcgtcatgcc acgctggtgg
     8221 ccctgcctgt tccgggacat ggtgcaggcg aaccgattgg aatactgacc cgcgtagatg
     8281 cgacgttctc ttctggttgc cagttgatga ttaacgctat tcgaaaatca atgccgttct
     8341 gaaaggtgaa gggatctgtc gatccctcct ttaacatttt cacaccggag cgaaactaac
     8401 tggttcaccc gctccgcgag gttctgccaa cacagattgt ttgttcagac ggaatacgtc
     8461 caccgcctca gttaatcgtg ccgcctgttc ttcaagggac accgccgccg ctgaggcctc
     8521 ttctaccaga gaagcattct gttgcgtcac cttatccatt tcagaaatcg cctggctaac
     8581 ctgcgttatg cccctacttt gctcatccga ggcggcggca atttcctgca tgatatgtgt
     8641 gacactcgcg acggcatcaa caatagtgct catggttttc ccggccgttg ccacctcatc
     8701 cgatcccagg tcaattaacc tgactgattc actgatcaag ccttcaattt cttttgccgc
     8761 ctgggcgctg cggcttgcga gtgtccgtac ttcgctggcg acaacggcaa atccacgccc
     8821 ttgctcaccc gctcgcgcag cttcaacggc agcattcagt gccagaatat tcgtctggaa
     8881 agcaatactg ttgatgacgg cggtgatctc agaaattttc ttcgaactcg tggagatagc
     8941 gcccatcgtt tttactacac cggaaaccgt ctgcccgcca tcgctggctt taatagaagc
     9001 ctcttgcgcc agtttgctgg cataatgcgc gttatccgca ttctgtttca ccgtcgcagt
     9061 gagttgctcc atgctggcgg cagtttgttc gatagccgcc gcttgttctt cggtgcgaga
     9121 tgacaggtcc gcattgccag ctgaaatttc gctggtgcca cgataaatct cttccgcacc
     9181 ctgtcgaaca gtccctacgg tcatccccag tgaatgctgc atttgctgta aatgacgact
     9241 taagcgaccg atttcattac gacccgccgg ttcatccttc atcgtcagat cgccactagc
     9301 gattttttca atccgttgtg cggcatgttg cagtggacga atgacgatcc gacgtagcac
     9361 cataaatgtt atcagcgtca tgaccagggc aagcacaaac gcaccaatca tgaacatccc
     9421 gcccaggcgg gtgcgctgat gggccagttc cgccagttgg ttggctctgg tgctacgtat
     9481 cttaacggct ttgttcaaaa tatcggtata agcattatcc agcgttcgga tctgctcact
     9541 ttcatgattg ataatcgctt caaacatgcc atttttggca tatttcaaca taggttgcat
     9601 acccgtgata taagcctgaa agcgttgatt taattcagtg tcgagggctt catcagcagg
     9661 tgttttcacc ggccgattct gataagcacg ataaccttgc tgcgactgtt taatctccga
     9721 ttcggcttgc gcaatatttc gcttcattgc ttccatttcc gcaatacgac tcgcagcccc
     9781 ggcctgaatc atattgatcc gggctgaacg caaaaaatct gaactgttcg ctaacccggt
     9841 ccgaatctca atctccgcag taacatcacg ctgatcgcga tcggcctgcc agaggaaata
     9901 gccagccagg gctgaactta atgcgaatag aactaagata ccgcctagaa tgcaggcaaa
     9961 taacggaacc aacctgatgt gatgcaaaaa acctaatcgc tgtgagggag ttgtattcat
    10021 taccgttgtc tctcgtccag gtttactcct gatagaaagt catcggcatt tgacggtaat
    10081 tacttacgac taaaagcctc tttgcgtcac atttttacaa caattattgc agagaagatt
    10141 aatgccattg gaatgaagag agtaacgaat ggttaaactg gctaacccga atgacgaaaa
    10201 attctggaaa acagcacgca atttcgtcag gtgattattt tcgtccctaa ggaaaccaca
    10261 taggatgctc acgggtgcct gagactttct gttttcaggc taaccgggaa tcagaaagac
    10321 gaaggccccg accgatattt ctatcaaccg aggccaacga atgaaccaag caattcagat
    10381 gttagcctct tacccgccat ccggcaagga gaaaggctat gaagcgcaac cctctggtgg
    10441 tgtgtctgct cattatctgc attacgattc tgacattcac actcctgacc cgacaaacgc
    10501 tctacgaact gcggttccgg gacagtgata aggaggttgc tgcgctcatg gcctgcacgt
    10561 ccaggtaagg gcaagcgcgg ggattttccc cgcgcatctg actagcagat tcattttctc
    10621 atggcacccc ttttacactt cagttagtgc atgttttttt cgatgcacta atagaaccat
    10681 cattacagac aaatttgccg cctgccgtac agtgtgagat cccccctttc gaaccagaac
    10741 agggtttgcg cccggcatcg gcataaaagg aaacaagcgc tcccataaac aaaataagtg
    10801 ccagtttttt catataacct ccatggttta tcgaatctta tgttcgattt gaacgttata
    10861 cgctggttta ttttgatacc gttgatgaga tcaaagatcg ggtgaattga ttaaaaataa
    10921 gccagatgga cgtatgggca aggattatgg ttttatttgt catacaaata aatataatag
    10981 gcgcttccga tgtagacccg tattcttcgc ctgtaccacg ggtcggtttt agtacaggcg
    11041 ttttctttaa atatccttca ggaacgttta cgcggcatca tgcgtagaag tgtgttgtcc
    11101 ttccagaaat agtggtgtgc cagtgcagcc gcagcgtgca aaccgatgac aaaatatccc
    11161 agattcgcca gcgtttcatg ccacgacttt aagctatcta cccgttcgaa attggcctct
    11221 gaagcgtaag gcatcgtcaa accaaacgca aaccacgggt tgcctcggtt atacatcatc
    11281 accaaaccaa tcactggcag cgcaataaac aggagataaa tcaccaaatg tcccaaatgc
    11341 gccagtcccg tcatcatcgg ttttggctta ggtataatcg gcggggttgg gtatttcagc
    11401 cttaacaaaa gacgaacgac catcagcacg agaattgata tgccgcagga aacatgaatc
    11461 atgttgatga gtggccgatc gctacgtggg aagaaacccc gaaactccat tgcgcaatac
    11521 gctgcgataa ccagcaaaaa gaccagccag tgaatgctga tttgtaacct tgagtattta
    11581 ttttccataa catttcctac tttaacataa ttttccgtta acataacggg cttttctcaa
    11641 aatttcatta aatattgttc accctttttc agtacgttac gtttcactgt aagaatggag
    11701 attgcccatc ccgccatcct ggtctaagcc tggaaaggat caattttcat ccgaacgttc
    11761 ctgacaggag aaaaccatga gtaaagttgg tattaacggt tttggtcgta tcggtcgact
    11821 ggtgttgcgt cgattacttg aagtcaaaag caacatagac gttgtcgcta ttaatgatct
    11881 tacttcccca aaaattctcg cctatctgct gaaacatgat tcaaactatg gtccgttccc
    11941 ctggagcgtt gattttacgg aagattcact tatcgttgat gggaaaagta tcgcggttta
    12001 cgccgaaaaa gaggctaaaa atattccgtg gaaagcgaaa ggtgcagaaa tcattgtcga
    12061 atgtactggc ttttatacct ccgccgagaa atcgcaggcg catcttgatg ctggcgcgaa
    12121 gaaggtgttg atttccgccc ctgcaggtga aatgaaaact atcgtttata aagtcaatga
    12181 cgacactctg gatggcaacg acaccattgt ttccgtggca tcatgcacta ctaactgtct
    12241 tgcgccgatg gccaaagcct tgcatgacag tttcgggata gaagtcggca cgatgacgac
    12301 cattcatgcc tatactggca cccagtcact ggtggatggc ccgcgtggta aagatttacg
    12361 tgcttcacgc gccgcggcag aaaatatcat tccccacact acgggcgcgg caaaagccat
    12421 tggtctggtg atcccggaac tgagcggcaa actgaaaggt catgcgcaac gcgtgccggt
    12481 gaaaacaggt tcggtcactg aactggtatc gattctcgga aaaaaagtga ctgccgaaga
    12541 ggtgaataac gcacttaaac aagcaaccac caataacgag tcatttggtt ataccgatga
    12601 agaaatagtc tcttccgata tcattggcag ccatttcggt tcggtgtttg atgccacgca
    12661 aacggaaatt accgccgtgg gcgatttaca actggtgaaa acggtcgcct ggtacgacaa
    12721 cgaatatggc ttcgtcacgc agcttattcg caccctcgaa aaattcgcta aactctgacg
    12781 cgcacaggcg gaggaaaaaa cctccgcctc tttcactcat taagactgta aataaaccac
    12841 ctgggtctgc agatattcat gcaggccatg tttaccatct gcgccgccaa taccggattt
    12901 acgccatccg gcgtggaagc cttgcatagc ttcgaagttt tcacggttga tgtaagtttc
    12961 accaaacttc agccctttaa tggctttcat cgcgacgttc agattttggg tatagattga
    13021 tgaggtcagg ccgtaatcac tgtcattagc cattgagata gcatcttcca gcgtgtcaaa
    13081 tgcgactacc ggcagaaccg ggccaaaggt ttcctcatgc ataatcgaca tttcctggcg
    13141 aacatccagc agcaatgtcg gcggataata atatcctttc ccctctaccg ctttgccacc
    13201 caacgccact ctcgcccctt cttctactgc gcgcgccact ttttgctcga ccctttccag
    13261 cgccgcggcg ttaatcaacg gccccatcgc aatgtcgttg cgttcagcgg ggttaccaaa
    13321 ttgaaccgcc tgcatcgctt cacccagccg attgacgaac tgatcataaa tgcctttctg
    13381 tacataaaca cgttctgcac agttacacac ttgcccacta ttaatgacgc gtgaatcaac
    13441 gatggctttg actgccagtt caagatcggc atcgtccatt acgatagctg gtgctttacc
    13501 ccccagttcc aggcacactt tggtgatgtt tttcgccgca gtcgccatga tcttctcacc
    13561 tgcagagacg ctgcctgtca tactgaccat tgcgaccttt gggttacccg ccagttcttg
    13621 cccaacggtt tcaccacgcc ccagaacaag gttaaacacg ccgcgcggaa ggcctatttc
    13681 atcgacgatt ttggcgaatg caatcgcatt gtttggcgta aattcactgg gtttaatgac
    13741 aatggtatta ccagtcaaaa gagcgggagc cattttgcgg gcaatgagga agaacgggaa
    13801 gttccacggc agaatgccgg tagtcacacc aagcgcacgt ttaaacagaa gaatattttc
    13861 tcctggacga tcgctttgaa taatctcgcc ctcgtaacgc cgtgcccact ccgccatgta
    13921 atcgatatag tcggcagtaa aagccacttc gacttcagcc agctgctgga tcttgccccc
    13981 ttcttcaaca atcagcgcac tgatttcact ggcgcgttcg cggatcccgg cggagatttt
    14041 gcgcaaccaa ctggcgcgtt caattgcagg caacgcttcc cattctggtt gtgcacgttc
    14101 tgctgcatcg attgccttac gggcatcctc ggcctgacca tcgggaatgc gggaaatgac
    14161 agcctctgta gcagggttta ccacatcaat ccatgcgtct ccacgccagg taacaaactg
    14221 tccatcaata tacataggat gttgaacggg tactgacatg ggcgactcct gtgatttata
    14281 tgtttgtttt tggcaaatac attgttaatt aacatcaatc atcatagaga cggcatcatg
    14341 aaaggacaat tctgtgaagg gcttcataaa aacaagtgta aaaggaataa gaggcagaaa
    14401 taaaaggcag gagttatgcc cgtttgcaaa caagtggttc acaaacgggc aatttttaac
    14461 gtaacgagcg actttccatc gcctcgatga gcaccgcatc gtgtttcagc gtttgccatg
    14521 catggatgac ttctgccgga aaatcaacgt gaacgataaa atctcgcccg cggggaccgt
    14581 agccatcgct atcatcgcgt aaacgcggca gctcgccagt gagtagtgag agataacgct
    14641 caaccggcca taatccttgt aactgattaa taaagattac actgtctgcg ttatttccta
    14701 actgaggaac gaatccggga taacttaacc agcgtggtac atcgggattg tccccggtca
    14761 tacggcggaa cgtcgccatc gtgcgccgct gcatggtggg gtcctgtaca acgatagctg
    14821 tgtgaactcg ttctacggcc tgattcaata gcgcgatgct aaagcgtgcg ttttcaccgc
    14881 agtttgttga ctggtcttca atccagattt tttcatgcgg aatatgccag aactgatgag
    14941 cgatatccgc caggatggtc gcttctgctc tgccagtggt gcggatagtg ttgtagtgcg
    15001 gatgctgtgc gatggcgcta tacaaaaaag tggtcgagtg accgatacca ccactaatca
    15061 gtaaaggaat ttgctgatcg cgagcaatct tacatgccgc atcgatagtc ggcataaccg
    15121 catttcctgc aagtatgacg caatcagcct gatacggcac ctcaccggag aaatcatcct
    15181 gcgccagcca ctgtccgata acatttatgg catctatagt tgccgtcgaa agcgtcggaa
    15241 acggggtaat gttcatggtt cctccttatc gttcccttaa gaatactgcg tgagataata
    15301 gtgagaagcg gagcactctg aaaggaagat aatgcttttt ctatctcgcc atcgcgaacc
    15361 agacactggt tttagtcata cactatttgc ggagaaactg ttgataaaac atcaataaca
    15421 tcatttgacg tggcaataac ataatgttcg atcattttat ttttatgaat ttcacaactt
    15481 tgcatagtaa accatgtaag ataagtggaa ttggctactc tatagatccc cgtttggtta
    15541 attccctgct ttcctgacat acctaacaaa tcaccttcgt cagtaacacg aaatgagtgt
    15601 atccagtcaa atgatatctt aacttctgac ttgatcgccg ggcatatcag ggccagttca
    15661 agcctggcat tcttatagct taaacctgac agatcggcgt gtttaaaaga caacgaagtc
    15721 ggcgtgatat ttataaaacg ttcatcca
//
LOCUS       DBFIFM010000108        15687 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.108, whole genome shotgun sequence.
ACCESSION   DBFIFM010000108 DBFIFM010000000
VERSION     DBFIFM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.108"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(227..670)
                     /gene="mioC"
                     /locus_tag="N7C74_002885"
     CDS             complement(227..670)
                     /gene="mioC"
                     /locus_tag="N7C74_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HGU2432718.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(760..1218)
                     /gene="asnC"
                     /locus_tag="N7C74_002886"
     CDS             complement(760..1218)
                     /gene="asnC"
                     /locus_tag="N7C74_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HGU2432719.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            1370..2362
                     /gene="asnA"
                     /locus_tag="N7C74_002887"
     CDS             1370..2362
                     /gene="asnA"
                     /locus_tag="N7C74_002887"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HGU2432720.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(2367..3818)
                     /gene="viaA"
                     /locus_tag="N7C74_002888"
     CDS             complement(2367..3818)
                     /gene="viaA"
                     /locus_tag="N7C74_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HGU2432721.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3812..5308)
                     /gene="ravA"
                     /locus_tag="N7C74_002889"
     CDS             complement(3812..5308)
                     /gene="ravA"
                     /locus_tag="N7C74_002889"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HGU2432722.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHNMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQRCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            5531..7399
                     /gene="kup"
                     /locus_tag="N7C74_002890"
     CDS             5531..7399
                     /gene="kup"
                     /locus_tag="N7C74_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HGU2432723.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANQEVLYALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            7566..7985
                     /gene="rbsD"
                     /locus_tag="N7C74_002891"
     CDS             7566..7985
                     /gene="rbsD"
                     /locus_tag="N7C74_002891"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HGU2432724.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKHHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            7993..9498
                     /gene="rbsA"
                     /locus_tag="N7C74_002892"
     CDS             7993..9498
                     /gene="rbsA"
                     /locus_tag="N7C74_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HGU2432725.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFESCDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            9503..10468
                     /gene="rbsC"
                     /locus_tag="N7C74_002893"
     CDS             9503..10468
                     /gene="rbsC"
                     /locus_tag="N7C74_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HGU2432726.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            10493..11383
                     /gene="rbsB"
                     /locus_tag="N7C74_002894"
     CDS             10493..11383
                     /gene="rbsB"
                     /locus_tag="N7C74_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HGU2432727.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            11509..12438
                     /gene="rbsK"
                     /locus_tag="N7C74_002895"
     CDS             11509..12438
                     /gene="rbsK"
                     /locus_tag="N7C74_002895"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HGU2432728.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            12442..13431
                     /gene="rbsR"
                     /locus_tag="N7C74_002896"
     CDS             12442..13431
                     /gene="rbsR"
                     /locus_tag="N7C74_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HGU2432729.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGYPIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(13397..14824)
                     /gene="mdtD"
                     /locus_tag="N7C74_002897"
     CDS             complement(13397..14824)
                     /gene="mdtD"
                     /locus_tag="N7C74_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HGU2432730.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPILGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNHVPSESE"
     gene            complement(14847..15539)
                     /gene="yieP"
                     /locus_tag="N7C74_002898"
     CDS             complement(14847..15539)
                     /gene="yieP"
                     /locus_tag="N7C74_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HGU2432731.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         3788 a         3834 c         4111 g         3954 t
ORIGIN      
        1 aggatcgcac tgccctgtgg ataacaagga tccggctttt aagatcaaca acctggaaag
       61 gatcattaac tgtgaatgat cggtgatcct ggaccgtata agctgggatc agaatgaggg
      121 gttatacaca actcaaaaac tgaacaacgg ttgttctttg gataactacc ggttgatcca
      181 agcttcctga cagagttatc cacagtagat cgcacgatct gtatacttat ttgagtaaat
      241 taatccacga tcccagccat tcttctgccg gatcttccgg aatgtcgtga tcaagaatgt
      301 tgatcttcag tgtttcgccc gtctgttttg caccggaatt tttgagttca gcctcgagtt
      361 tatcgatagc cccacaaaat gtgtcatatt cacgactacc aataccgatt gcgccaaagc
      421 ggaccgcaga aagatcgggc ttctgttcct gcaatgcttc atagaaagga gaaaggttgt
      481 ccggaatatc tccggcaccg tgggtggagc tgataaccag ccagatcccc gaggcagata
      541 aatcttccaa cagcggaccg tgcagcgttt cggtggtaaa acccgcctct tccagctttt
      601 cagccaggtg ttctgctaca tattcggcac cgccgagggt gctgccgctg ataagagtta
      661 tatctgccat aaaccgccac ctttattaag agtggcgtat tgtacgctgt gaacgcgttg
      721 ggatctacct gtggaaaagt atgggattaa aaaagccgat cagggcttga tggtacgcat
      781 gatcgggttc tgcaggacga tcagtgtctc ggtggactga atttcatcaa ttgtttggat
      841 cttgttgata agtacatgct ggagagcatc gatcgaacgg cacatcactt ttataaagat
      901 gctgtagtgg ccggtggtgt agtacgcctc ggtcacttca tcgaggcttt ccagctttgc
      961 cagcgcggaa gggtagtctt tggcgctctt taatataatg ccgataaagc agcctacgtc
     1021 ataaccgagc tgcttcgggc tgacatcaat acgcgccccg gtaatgatcc ccgcctgctt
     1081 cattttctct actcgaacgt gaatcgtccc cggactgacg ccaaattgtt tcgccagttc
     1141 ggcgtaagcg gtgcgcgcat tgcccattaa tgcttccagg atgccacggt ccagattgtc
     1201 gatcagataa ttttccatag gattttctta tgcggattga tgattcattc tattttagcc
     1261 ttctttttta atgaatcaaa agtgagttag gctttttatt gaatgattat tgcatgtgtg
     1321 tcggtttttg ttgcttaatc ataagcaaca ggacgcagga gtataaaaaa tgaaaaccgc
     1381 ttacattgcc aaacaacgcc aaattagctt cgtgaaatct cacttttctc gtcaactgga
     1441 agaacgtctg gggctgatcg aagtccaggc gccgattctt agccgtgtgg gggatggcac
     1501 gcaggataac ttgtcgggct gtgaaaaagc ggtacaggta aaagtgaaag ctctgcctga
     1561 tgcccagttc gaagtggttc attcactggc gaagtggaaa cgtcagacct tagggcaaca
     1621 cgacttcagc gcgggcgaag ggctgtacac gcacatgaaa gcccttcgcc ccgatgaaga
     1681 ccgtctttct ccgttgcact cggtctatgt tgaccagtgg gactgggaac gcgtaatggg
     1741 cgacggtgag cgtcaattct cgactctgaa aagcacggta gaggcgattt gggcgggaat
     1801 taaagcaacc gaagctgcgg ttagcgaaga gtttggcctg gcaccgttcc tgccggatca
     1861 gatccacttc gtacacagcc aggagttact gtctcgttat ccggatcttg atgccaaagg
     1921 gcgtgagcgg gcgatagcga aagatcttgg tgcggtattc cttgtcggga ttggcggcaa
     1981 gctgagcgat ggtcatcgcc acgacgtgcg cgcaccggat tatgatgact ggagcacccc
     2041 gtcagagctg ggccatgcgg gtctgaacgg cgatattctg gtgtggaacc ctgtactgga
     2101 agatgcgttt gagctttctt ccatggggat ccgcgtggat gccgacacgt tgaagcatca
     2161 gttggcgctg accggtgatg aagatcgtct gcagctggaa tggcatcagg cgttgctgcg
     2221 cggtgaaatg ccgcagacca tcggcggcgg tatcggccag tctcgtttga ccatgctgct
     2281 gctgcaactg ccgcatatcg gccaggtcca gtgtggagta tggccagctg ctgttcgcga
     2341 gagcgtccct tctctgctgt aataatttat cgccgccagc gtctgagcag gcggcttcgc
     2401 atcccggtat caaagcgcca gatatgatcg aaaatgcgca tgatgccggg tttgccgtgt
     2461 gccgacatcg ccacggcatg aaagcgatgc tgatgtaccc gctgcagctc tttcacttta
     2521 ctcgtcacgt cgtcaggcaa ccgctgggcg ataaaatcag aaatcaccac cgcatcggca
     2581 tcaaaccatt ccctgctttg caagcgttcc ataatggcgc gaaaacaact ggcaagatcg
     2641 gtgccgccac gaaactgctg gcttaaaaaa cggattgctt gttcgatgcc ttgtgggcct
     2701 gaaagctcat aacggacgat ctcggtggaa aatagcataa tataacagcg ccggttttca
     2761 gcgagagcaa tgcgcatcaa agccaggcag aacgctttcg cacactgttc attaaagccg
     2821 cccattgagc cggaagtatc cacgcagaca ataaacggtc cgcgcggctg ttcgtcgtaa
     2881 tctttatgca ccaccgggcg ttcgatcact ttttcacgcc acgactcacc gtgcaggcga
     2941 taggtgagca actgtttttc caccagccga cggtaaaact catactccag ttccgttatc
     3001 cctagtgtcg ccagttctgg cggcagaaga cgtaaaatat catcgctttg ttgcagacca
     3061 tcaacctgct caggaaccgt cgccggttcg cgcaccatgg tgcggaaggt ttccatctgc
     3121 gcatcgttgc gcggtattga tttggcttcc cgggaacgcc ccagttgttc tgccaggcgt
     3181 ttcagttccg gctgttcgtt aagaaattca ccgtatttca caatcaactg atagtcgcca
     3241 cgtttaagct gaccggcgct catatcccac agacgaccag ctgcggtatt gttatctgcg
     3301 agaatcggtt caagttgtcc gctcagcgtc atgcgttcct gaacttcact caacagttgt
     3361 tcgcgttctt cttctaatag ctgttgattt aacgttgttg cttgcacgat cagacttaaa
     3421 cgccagcgct ggagaaaaag cgtgtgtaac gctgaagtga tcgtgctgtt agcatcgacc
     3481 aactgtcggg cttgttctgc ccatggggaa ttcagacgat gcagtaagtc caggatctgt
     3541 ggtagctgca caataaactg cggcgtggag aggagctggc tttgctgata gcacatcacc
     3601 tcttcggtga gttccggcgg gactcgggca tctttcagcc gactgcgcag cgcctcacgc
     3661 cagcggggaa catcatcagt gattgccgcc ttcagtcgtg ggaatttttc aaagaagact
     3721 gccagctgcg gtgaggccag cagcgcgatg atcatctctt cgatcaatcc ctcttcgctg
     3781 acggccagca tcacattaag cgtatccagc gttagcattg ttgtgcctgg cgaatctgtg
     3841 cgccgacatc ctgtaggctg gcttcaatgc gacctaacca gtcgccaggg ataaacagac
     3901 atctttgctg ttcgctgaat aaagcgtgtt gcttgcgcca gtcgctttca agctcttcca
     3961 attgttgttt tatttcacct ggcagacctt cagccgatga accgggaagt gccagcgtac
     4021 tgccttgtaa acttacatcg cgtacaacaa gatgttgggc gctatcaact tccagattca
     4081 gtttctgggc aaagccgata ccgttcagtt tgccgcgaat ttcaccacct ttgctcagcc
     4141 actgttccag cgcgctacgc tcaaaggaga tatgaaccac ttccatatta tgcaatttta
     4201 acggtttttg cagcagcaga gtcagagtgg aagcagtaac gttaacagga agttgatact
     4261 gctgacgacg gctgaaaatg ccgcccagac gaattaccgt taaggctgtt ttatcgcttt
     4321 gttgctgctg gagttgcagg tgacgttgca caatcgcgcc caggcgggtc aacatccctt
     4381 gctgttgcca ggcatgaccg gtcatcaata catcaatttg ttgttgtatc aaattcaggc
     4441 tttgcgcgtc ataccacagg caatctttca gcaaaatgag atctaccggg gcaacagcac
     4501 tgcgaccgct aaaaaaggcg ctggcctgca ataatcgaat cgcttttttc cagcgacgat
     4561 ccgagacata aggcgcatcc ggtaatttat ccagttgctg gcgcagcata aaaatcagct
     4621 caaatacatg atcgggcagc gtaatttcac caatctcttt ttgccagcgt tcatattctt
     4681 catctgtgac ctgcaaggag gctggaaccg gattgtcatt ttcatcctgt tgactggtca
     4741 gcatggagcg gaaattcgct ttatcctgca ctttatctaa ccacagacga atcagcatgc
     4801 ggtcatataa cgcttccaga ctgctgtctg cttccggcag ctcgttggag gccgccacca
     4861 gcaggcgcat cgggattttt tctacgtgtg caccgttgcg gaactggcgc tcgttaatgg
     4921 cggtgagcag ggtattaaga attgccggac ccgctttcca gatctcatcc agaaagacga
     4981 tttctgcttc cggcaggtaa ccgctggtta aacgttcata gcgcccttca tcttttagcg
     5041 cctgaataga aaggggacca aaaacttctt ccggcgtgga gaagcgggtc atcagatatt
     5101 caaacgcgcg ggcattctga aaggcgaatt ttaagcgccg ggcgatcaaa cttttggcaa
     5161 tacctggcgg gccaaggagg aacacacttt caccacttaa tgccgctaat aaacacaagc
     5221 ggatggcgtg gctacgttca taaagcccct tttccaacga actgctcagg cgggaaattc
     5281 tttccgctaa taaatgaggg tgagccataa tgaagtggcg tcctttcgtc aaaagttctg
     5341 cgtaaattgc gagtatagac gtttcttgct ggtggctaaa atagtctcaa agggggggta
     5401 tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc cgttaatcgt
     5461 gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata tttcaacgaa
     5521 agactagtct atgagcactg ataataagca atcattgccc gcgattaccc tcgcggcgat
     5581 tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg aatgtttgtc
     5641 cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat cgctgatctt
     5701 ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc gggcagataa
     5761 cgccggtgaa ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata cgtcggcgcg
     5821 aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct atggtgaagt
     5881 cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa tcgtcgcccc
     5941 gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat tatttatgat
     6001 tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc tgacctggtt
     6061 tttgattctg gcaggactgg ggttacgtag cattattgct aaccaggaag tgctgtatgc
     6121 gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt cttttattgc
     6181 attaggggca gtggtgctgt cgattacggg gggcgaggcg ctgtatgctg atatggggca
     6241 ctttggtaag ttccctattc gcctggcgtg gttcaccgtc gtattacctt ccttgaccct
     6301 taattacttc ggtcagggag cgctgttgtt aaagaatccg gaagcgatta agaacccgtt
     6361 cttccttttg gcaccggact gggcgctgat cccgctgctg atcatcgccg cactggcgac
     6421 ggtaatcgcc tcgcaggcgg ttatctctgg cgtcttttca ttgacgcgtc aggcggtacg
     6481 tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt cagggcaaat
     6541 ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga ttgtcagctt
     6601 tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa ccatggtgct
     6661 gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata agtattttgt
     6721 tgcgctgatc ttgattgctt tcctttgtgt cgatattcca ttgttcaccg ctaacctcga
     6781 taaactgctc tccggcggct ggttgccatt gagcctcggt acggtgatgt ttatcgtgat
     6841 gaccacctgg aagagtgagc gtttccgctt gctgcggcgg atgcatgaac atggtaactc
     6901 tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc ccgggaccgc
     6961 ggtatatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata accttaaaca
     7021 taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag acgctccata
     7081 tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct ggcgcgtggt
     7141 ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc gctgcggtct
     7201 ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg agtcgttgat
     7261 cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc tgctgcaacg
     7321 taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta tcgaactggg
     7381 tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc gtttttgttt
     7441 tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta acgtcacgat
     7501 ggttttccca actcagtcag gattaaactg cgggtcagcg aaacgtttcg ctgatggaga
     7561 aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc tcccgtctgg
     7621 gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa agtacaacgc
     7681 gtatcgatat ggcattaacc cagggtgtac cttcttttat gcaggtgctg ggcgtcgtca
     7741 caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacac cataatccgc
     7801 aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag ggaaatacca
     7861 ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa agtcaggcgg
     7921 taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct ggcgtgacgt
     7981 tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc cttcccgggc
     8041 gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgcgtgat ggcgctggtg
     8101 ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggcat ctatactcgc
     8161 gatgccggta cgcttttatg gctggggaaa gaaacgacat ttaccgggcc gaaatcttcc
     8221 caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca gttaaccatt
     8281 gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat tgactggaaa
     8341 accatgtatg ccgaagcgga taaattgctg gctaaactta acctgcgctt taaaagcgac
     8401 aagctggtgg gcgatctttc catcggtgac cagcaaatgg ttgaaatagc caaagtgctg
     8461 agctttgagt cgaaagtcat cattatggat gaaccgaccg atgcgctgac cgataccgaa
     8521 accgaatccc tgttccgcgt catccgcgag ctgaaatcgc aaggccgcgg tattgtctat
     8581 atctcccacc gcatgaaaga aatcttcgag agttgcgatg acgttaccgt ttttcgtgac
     8641 ggacaattta ttgctgagcg cgaagtggca tcactgaccg aagattcgct gattgagatg
     8701 atggtgggtc gcaagctgga agatcaatat ccgcacctgg acaaagcgcc gggagatatc
     8761 cgcctgaaag tcgataatct ctgcggacct ggcgttaacg atgtctcttt tactttacgc
     8821 aaaggcgaaa ttcttggcgt ctctggtttg atgggcgcag gtcgtaccga actgatgaaa
     8881 gtgctctacg gcgcactacc gcgcaccagc ggttacgtca ccctggatgg gcatgaagtc
     8941 gttacccgtt caccgcagga tggcctggca aacggcattg tgtatatctc cgaagaccgt
     9001 aaacgtgacg gtttagtgtt gggcatgtca gtaaaagaga acatgtcgct gacagcgctg
     9061 cgctacttca gccgcgctgg cggcagtttg aagcatgccg atgaacagca ggctgtgagt
     9121 gattttattc gcctgttcaa tgtgaaaacg ccgtcgatgg agcaggcaat tggtctgctt
     9181 tccggtggca atcagcaaaa agtggcgatt gcccgcggtc tgatgacacg ccccaaagtg
     9241 ttgatccttg atgaacctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa
     9301 ctgattaacc agttcaaagc cgatggcttg agcatcattc tggtgtcatc ggagatgcca
     9361 gaagtattag gcatgagcga tcgcatcatc gtcatgcatg aagggcatct cagcggggaa
     9421 tttactcgtg agcaggccac ccaggaagtg ttaatggctg ccgctgtggg caagcttaat
     9481 cgcgtgaatc aggagtaaaa aaatgacaac ccagactgtc tctggtcgcc gttatttcac
     9541 gaaagcgtgg ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt
     9601 ctcgacgtta agcccgaact ttttcaccat caataactta ttcaatattc tccagcaaac
     9661 ctcagtgaac gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggcatcga
     9721 cttgtcggta ggttctctgt tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat
     9781 tgaagtcaat gcgctggtgg ctgtcgctgc cgctctcgcg ttaggtgccg caattggtgc
     9841 ggtaaccgga gtgattgtag cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat
     9901 gatgctttta ctgcgcggcg tgaccatggt ttataccaac ggtagcccag tgaataccgg
     9961 ctttactgag aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc
    10021 gacgccagtc tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac
    10081 gcgtctgggg cgttacatct acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg
    10141 tatcaacgtc aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcact
    10201 ggccgggatc attgaagtgg cgcgtctctc ctccgcacaa cccacggcgg ggactggcta
    10261 tgagctggat gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg
    10321 tcgcattgtt gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa
    10381 tttgttaggt gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc
    10441 ggtgctggta gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat
    10501 gaaaaaactg gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc
    10561 gatggcaaaa gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc
    10621 gctgaaagat ggcgcgcaga aagaggcgga taaacttggc tataacctgg tggtgctgga
    10681 ctcccagaac aacccggcga aagagctggc gaacgtgcag gacttaaccg ttcgcggcac
    10741 caaaattctg ctgattaacc cgaccgactc cgacgcagta ggtaatgctg tgaagatggc
    10801 taaccaggcg aacatcccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt
    10861 gagccacatt gcttctgata acgtactggg tggcaaaatc gccggtgatt acatcgcgaa
    10921 gaaagcgggt gaaggtgcca aagttatcga gctgcaaggc attgctggta catccgcagc
    10981 ccgtgaacgt ggcgaaggct tccagcaggc cgttgctgct cacaagttta atgttcttgc
    11041 cagccagcca gcagattttg atcgcaccaa aggtttgaac gtaatgcaga acctgttgac
    11101 cgctcatccg gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct
    11161 gcgcgcactg caaactgccg gtaaatcgga tgtgatggtc gtcggatttg acggtacacc
    11221 ggatggcgaa aaagcggtga atgatggcaa actagcagcg actatcgctc agctacccga
    11281 tcagattggc gcgaaaggcg ttgaaaccgc agataaagtg ctgaaaggcg agaaagttca
    11341 ggctaagtat ccggttgatc tgaaactggt tgttaagcag tagttttaat caggttgtat
    11401 gacctgatgg tgacataaat acgtcatcga cagatgaacg tgtaatataa agaaaagcag
    11461 ggcacgcgcc accctaacac ggtggcgcat tttatggaca tcccgaatat gcaaaacgca
    11521 ggcagcctcg ttgttcttgg cagcattaat gctgaccaca ttcttaatct tcaatctttt
    11581 cctactccag gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg cggcaaaggc
    11641 gcgaatcagg ctgtggctgc tgggcgtagc ggtgcgaata tcgcgtttat tgcctgtact
    11701 ggcgatgaca gcattggcga gagcgttcgc cagcagctcg ccactgataa catcgatatt
    11761 actccggtca gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt tgttaatggc
    11821 gaaggtgaga atgtcatcgg tattcatgcc ggcgctaatg ctgccctttc cccggcgctg
    11881 gtggaagcgc aacgtgagcg tattgccaac gcgtcagcat tattaatgca gctggaatca
    11941 ccactcgaaa gtgtgatggc agcggcgaaa atcgcccatc aaaataagac tatcgttgcg
    12001 cttaacccgg ctccggctcg cgaacttcct gacgaactgc tggcgctggt ggacattatt
    12061 acgccaaacg aaacggaagc agaaaagctc accggtattc gtgttgaaaa tgatgaagat
    12121 gcagcgaagg cggcgcaggt actgcatgaa aaaggtatcc gtactgtact gattacttta
    12181 ggaagtcgtg gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg
    12241 gtgcaggctg tcgataccat tgctgccgga gataccttta acggcgcgtt aatcacggcg
    12301 ttgctggaag aaaaaccatt gccagaggcg attcggtttg cccatgctgc cgctgcgatt
    12361 gccgtaacac gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat cgacgcattt
    12421 ttagacaggc agaggtgacg cttggctaca atgaaagatg ttgcccgcct ggcgggcgtt
    12481 tctacctcaa cagtttctca cgttatcaat aaagatcgct tcgtcagtga agcgattacc
    12541 gccaaagttg aagcggcgat taaagaactc aattacgcgc catcagctct ggcgcgtagc
    12601 ctcaaactca atcaaacaca taccattggc atgttgatca ctgccagtac caatcctttc
    12661 tattcagaac tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc
    12721 ctttgcaata ccgaaggcga tgaacagcgg atgaatcgca atctggaaac gctgatgcaa
    12781 aaacgcgttg atggcttgct gttactgtgc accgaaacgc atcaaccttc gcgtgaaatc
    12841 atgcaacgtt atccgacagt gcctactgtg atgatggact gggctccgtt cgatggcgac
    12901 agcgatctta ttcaggataa ctcgttgctg ggcggagact tagcaacgca atatctgatc
    12961 gataaaggtc atacccgtat cgcctgtatt accggcccgc tggataaaac tccggcgcgc
    13021 ctgcggttgg aaggttatcg ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc
    13081 tatgaagtca ctggtgattt tgaatttaac ggcgggtttg acgctatgcg ccaactgcta
    13141 tcacatccgc tgcgtcctca ggccgtcttt accggaaatg acgctatggc tgttggcgtt
    13201 taccaggcgt tatatcaggc agagttacag gttccgcagg atatcgcggt gattggctat
    13261 gacgatatcg aactggcaag ctttatgacg ccaccattaa ccacgatcca tcaaccaaaa
    13321 gatgaactgg gttatccgat tgatgtactc atccatcgga taacccagcc gacccttcag
    13381 caacaacgat tacaacttac tccgattctg atggaacgtg gttcggctta gatttacgct
    13441 gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt gctgctgaag
    13501 caacagtaat aatgcccatt gtgataaacg tatagtggaa ttgttcgaca gtcgttgtgc
    13561 cttccattcc ttcataaacg cgaaggacgg ccgcacttac agctacgcct aaactgatag
    13621 acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca tcggtcagat
    13681 cggcaagtgt gatggtattc atcgcggtaa actgcgtcga catagccatc cctaatataa
    13741 acaacggcaa gatcagcatc catattgcca ttgccggtga ttgcaaagag aactgagcga
    13801 tcattagccc aataatcacc gtgatcccca ctaatgtatg gcgatagccc agacgacgta
    13861 agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt gccatcatac
    13921 agccagcaat aaacgcctga taaccaaatc ctacctgtaa cattaatggc ataaggaacg
    13981 gtacacagcc ggtccccaga cgggttgcaa tattgcctac gataccgatc gagaaagtgc
    14041 gggttttaaa taaatctaat gaaattaatg ggtttggcgt gcgtcgtgca tggagaatat
    14101 agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag ctggcgacaa
    14161 tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg ccaaacagca
    14221 aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata tgtttgcgcg
    14281 cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag tgccaggttg
    14341 cccaggtgac cagcacgccg ccaagaatgg ggcctaaaat tggccccacc agacccggca
    14401 tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc agtaaggcca
    14461 gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg aagacaacca
    14521 gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac agactcacgg
    14581 caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt accggaataa
    14641 gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga ggagaacgat
    14701 taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca agtgcctgca
    14761 tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc tttttatcgc
    14821 tcattcaatg tcctgttatc gggttatcac ttatcaggtg agcgtagcag cgcctgacaa
    14881 gctttaaatg ccgcgtcgcc atcgctttgg ataatcgcat cgactatcgc ctgatgcaga
    14941 tccagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata aaccgaatgg
    15001 aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat atgctcatgc
    15061 caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa tgccgccatt
    15121 tcggccatta acgtattgag atgcgctttc tgttccgcgg tgccaaccgt tgctgccagt
    15181 aggcaagctt gtggctccag acagatacgc ataaccagaa agtgatcgat gacctgatga
    15241 aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt ccagttcgat
    15301 tgtggcatga cccgggtacc aattcgcggt cgcggtaaaa ccatcccttt tgccgttaac
    15361 gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc cagctcaatc
    15421 tcaccgggca aaatggtgcc gggttcatat tcacctttta agatccgttg cgccagcttc
    15481 tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct taaaggcatt
    15541 actaatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt gtgattacgg
    15601 ttgcaaaaac ggcaaattgc ttgttttatg gcacattaac ggggcttttg ctgaaaaaat
    15661 gcgcggtcag aaaattattt taaattt
//
LOCUS       DBFIFM010000109        15545 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.109, whole genome shotgun sequence.
ACCESSION   DBFIFM010000109 DBFIFM010000000
VERSION     DBFIFM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.109"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..180)
                     /locus_tag="N7C74_002899"
     CDS             complement(<1..180)
                     /locus_tag="N7C74_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432732.1"
                     /translation="MLHIISPRGISPRQRRLFYPFSSALALFYYAAAFTSGTMLSIST
                     QYVVLLVTNIITINRH"
     gene            323..538
                     /locus_tag="N7C74_002900"
     CDS             323..538
                     /locus_tag="N7C74_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU2432733.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVSGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            538..1035
                     /gene="rrrD"
                     /locus_tag="N7C74_002901"
     CDS             538..1035
                     /gene="rrrD"
                     /locus_tag="N7C74_002901"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HGU2432734.1"
                     /translation="MSPSLRKAVAAAIGGGAAAIASVLITGPSGDDGLEGVSYIPYED
                     IVGVWTVCHGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            1032..1499
                     /locus_tag="N7C74_002902"
     CDS             1032..1499
                     /locus_tag="N7C74_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU2432735.1"
                     /translation="MSRVTAIISALVICIIVCQSWAVNHYRDNAIAYKEQRDKKVSEL
                     KQATATITDMQQRQRAADALDAKYTKELADAKAKNDALRRKLDNGGRVFVKGKCPVPS
                     SDETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR"
     gene            1487..1639
                     /locus_tag="N7C74_002903"
     CDS             1487..1639
                     /locus_tag="N7C74_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432736.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGAHFWSESWLNISLLMLDIEHLA
                     RGKGLR"
     gene            2314..2805
                     /locus_tag="N7C74_002904"
     CDS             2314..2805
                     /locus_tag="N7C74_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HGU2432737.1"
                     /translation="MDHELKNLVLNINQLAALSGLHRQTVVARLKNIRPAGGHDKLKL
                     YRLTDILTEFMGLPPPVAEGEMDPHERKAWYQSERERLKFEQETAQLIPASDVRREFA
                     IWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEELQ
                     QEE"
     gene            2805..4907
                     /locus_tag="N7C74_002905"
     CDS             2805..4907
                     /locus_tag="N7C74_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGU2432738.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLARTFRVSPEVVSRLSPNKNDNNVYDRTFLAGNYLKIGWPSVNI
                     MSSSDYKCVALTDYDRFPEDIDGEGDAFSLASKRTTTFMSSGMTLVESSPGRDVKDVK
                     WRRTSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPCGDVVAGFRDIADPVLAS
                     EAAYIQCPSCSGRIMPEQKRELNGRGVWLRDGESINADGSRYGDPRRSRIASFWMEGP
                     AAAYQTLSQLVYKLLTAEQEYETTGSEETLKTVINTDWGLPYLPRASMEQRKSELLEQ
                     RAEPVPSSSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRG
                     DSDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDN
                     AYKFWRRCRRDGLGKRIYLFKGDSIRRAKLITRTFPDNTGRTGRRAQAAGDVPLWLLQ
                     TDALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAF
                     DLMVYAEALVILHGYEKIRWPDAPGWASRETWLESVPDSTEPSPSPEPVSTPVKKQKR
                     KKTVTDDVNPWLTSGGWL"
     gene            4904..5116
                     /locus_tag="N7C74_002906"
     CDS             4904..5116
                     /locus_tag="N7C74_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432739.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            5044..6168
                     /locus_tag="N7C74_002907"
     CDS             5044..6168
                     /locus_tag="N7C74_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU2432740.1"
                     /translation="MGAPPCGSDYTTTGASRVPAGEVLMAILDDVIGVFSPGWKAARL
                     RSRAVIQAYEAVKTTRTHKARRENRTADQLSQYGAVSLREQARYLDNNHDLVIGVFDK
                     LEERVVGKNGIIVEPHPVLRNGAIARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLM
                     LRTWLRDGEVFAQMVSGRINSLTPSAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDD
                     WGRPEKYLVYKSRPVSGRQMETKEVDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSAL
                     KEYEDSELTAARIAAALGMYIRKGDGQSYEPDGNGSKDKERELTIQPGIIYDDLKPGE
                     EIGMVKSDRPNPNLETFRNGQLRAVAAGSRLNFFQYSAQL"
     gene            6290..6625
                     /locus_tag="N7C74_002908"
     CDS             6290..6625
                     /locus_tag="N7C74_002908"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU2432741.1"
                     /translation="MASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIR
                     GGAATESDWVRAGGRNPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEP
                     QHTDDQSEE"
     gene            6570..8597
                     /locus_tag="N7C74_002909"
     CDS             6570..8597
                     /locus_tag="N7C74_002909"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HGU2432742.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLNINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGKPVIMPENSFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDGPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQTLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            8684..9007
                     /locus_tag="N7C74_002910"
     CDS             8684..9007
                     /locus_tag="N7C74_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HGU2432743.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGMTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            9000..9275
                     /locus_tag="N7C74_002911"
     CDS             9000..9275
                     /locus_tag="N7C74_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HGU2432744.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            9287..9865
                     /locus_tag="N7C74_002912"
     CDS             9287..9865
                     /locus_tag="N7C74_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2432745.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAGSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLAQR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGPLTQ
                     AFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            9862..10263
                     /locus_tag="N7C74_002913"
     CDS             9862..10263
                     /locus_tag="N7C74_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGU2432746.1"
                     /translation="MNRHTQIRQVVLARLREQCGDSATFFDGLPAFVDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVSALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            10274..11017
                     /locus_tag="N7C74_002914"
     CDS             10274..11017
                     /locus_tag="N7C74_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2432747.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASASVNVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTADGNFVAVAAVTVSAAG"
     gene            11078..11464
                     /locus_tag="N7C74_002915"
     CDS             11078..11464
                     /locus_tag="N7C74_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU2432748.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAEEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIENAGDLEHEVLTPEKS"
     gene            11473..11802
                     /locus_tag="N7C74_002916"
     CDS             11473..11802
                     /locus_tag="N7C74_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU2432749.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            11774..14839
                     /locus_tag="N7C74_002917"
     CDS             11774..14839
                     /locus_tag="N7C74_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU2432750.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASVHAQTVEKSAQAHERMAREVEQTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMPGAVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEARKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAETAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFSPQ
                     YNIEINNDGTNGQIGPAALKAVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            14839..15168
                     /locus_tag="N7C74_002918"
     CDS             14839..15168
                     /locus_tag="N7C74_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2432751.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            15178..>15545
                     /locus_tag="N7C74_002919"
     CDS             15178..>15545
                     /locus_tag="N7C74_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU2432752.1"
                     /translation="MQDIPQETHHETTRLTQSAQAVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSSARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGN"
BASE COUNT         3821 a         3589 c         4640 g         3495 t
ORIGIN      
        1 atgtcgatta atcgttatga tattggttac aagaagtacc acgtattgtg ttgagataga
       61 aagcatggtg ccagaggtaa atgcagcagc ataataaaaa agagccagcg cagaagagaa
      121 cgggtaaaag agtctgcgct ggcgtgggga tattccccgt ggagaaatga tatgtaacac
      181 acatcgggaa cctttctata taaacattat cattattgtc aatcataaca gtcaggtatt
      241 atgacgttta tgcatcaggg ccatcaggaa ttaactggtg gctttttatt gttgtcagct
      301 tccggataac gggagacggg gtatgtacca gatggaaaaa ataacaacag gtgtgtcata
      361 caccacgtca gcggtgggga cgggatactg gctactgcag ttgctggaca aagtctcccc
      421 atcccagtgg gtggcaatag gcgtatcggg tagcttggtg tttggcttgc tgacgtatct
      481 gacaaacctt tatttcaaga ttaaagaaga taagcgtaag gctgcgagag gtgaataatg
      541 tcgccatcat tacgcaaggc tgttgctgct gctattggtg gtggggctgc tgccatagcg
      601 tctgtgctca tcactggtcc gagtggtgac gatggcctgg aaggtgtcag ctacatacca
      661 tacgaagata tcgttggcgt atggactgta tgtcacggac acaccggaaa agacatcatt
      721 cccggtaaaa cgtataccga agcagaatgc aaagccctcc tgaataaaga ccttgccacg
      781 gtcgccagac aaattaaccc gtacatcaaa gtcgatatac cggaaacaac gcgcggcgct
      841 ctttactcgt tcgtttacaa cgtgggcgct ggtaatttca gaacatcgac gcttcttcgc
      901 aaaataaacc agggtgatat caaaggcgca tgtgatcagc tacggcgctg gacatacgct
      961 ggcggtaagc aatggaaagg gctgatgact cgccgcgaga ttgagcgtga agtctgtttg
     1021 tgggggcaac aatgagcaga gtaaccgcga ttatctccgc tctggttatc tgcatcatcg
     1081 tctgccagtc atgggcggtt aatcattacc gtgataacgc catcgcctat aaagaacagc
     1141 gtgataaaaa agtcagtgag ctgaagcagg cgaccgccac cattactgac atgcagcagc
     1201 gccagcgtgc tgctgatgca ctcgatgcta aatacacgaa ggagttagct gatgcgaaag
     1261 ctaaaaatga tgctcttcgg cgcaagcttg ataatggtgg tcgggtgttc gtcaaaggaa
     1321 aatgccctgt gccatcctca gacgaaacct ccagcgcctc cggcatgggc aatgatgcca
     1381 ccgtcgaact ctctccagtt gctggacgaa acgttctcgg tatccgggac ggaattatcc
     1441 gcgaccaaac agcactgaga acgcttcagg aatacatcag gacgcaatgc cttcgatgat
     1501 agcgataatt ttactcatca tccttcacat ctggctctgt agacagggtg gtgctcactt
     1561 ctggagtgaa tcatggttaa acatctcatt gctgatgctt gatattgagc atctggcgcg
     1621 cggtaagggg ctgcgttgag ataagagcca gtcattaaaa atacctggat ttagcctcgc
     1681 attcgcgggg cttttttatt gccattacaa aagccactcc ctacagagtg gctttgataa
     1741 tggcttatac cctacacggg ataacttaac tgatatccct tttaacggat aaaggtattc
     1801 aagcctgaca catcatgcgc tgtatcgtcg ctgtattccc gcattaacca tgaccgtagc
     1861 ccgacgggga actccttctg cgcgagtgtg cgggaataat caaaaacgat gcacaccggg
     1921 tttttaccgc gctaatgatt cgcgggtttg tccctcatgc tcgccagtcc tgtgcggggg
     1981 tggaagaaac aggacactca cacagattct tgtgggtcga tgctattcct ttctggatta
     2041 tcccgatgcc attcatgcaa gggctgtatc agacgttcgt catggctttc aggctgacgg
     2101 ctcctcccgg tggggtggcc tgccacgggg cgggagcgtc gcggaaaaag gctagttttt
     2161 gcatttttat cggccaccat catctttgca tcttattgat tattaatggt tatttgtttt
     2221 ttgtatgtcg aattgagtgt tttttgttcg acatcgaacg cgttttctta aagttgttcg
     2281 cacgatgcat gtttaaagct ctccggagga aatatggatc atgagttgaa aaacctggtg
     2341 ctgaatatta atcaactggc ggctttatct ggtctgcacc gccagactgt cgtggcaaga
     2401 ctgaaaaaca ttcgtcccgc tggtggacat gacaaactca agctataccg gttgaccgat
     2461 attctgactg aatttatggg gttaccaccg ccggttgctg agggcgaaat ggatccacat
     2521 gaacgcaaag cctggtatca gtctgaacgt gagcgtctta agttcgaaca ggaaacggca
     2581 caactcattc cggccagtga tgtcagacgg gagtttgcca tctgggcaaa agcggtcgtg
     2641 caggtgctgg agacattacc ggatattctg gaacgtgact gtggtctgca gcctgccgct
     2701 gtgagccgtg ttcagtccat tattgatgat ctgcgcgatc agatagccct gcgggtgacc
     2761 gaagcaggtg cggatgatga ggaggaatta cagcaggagg agtaatgctg aatcaggaaa
     2821 ccgcaaaggc agcacgaacc gattcaggtt atatccttcg cgcaccgaga cgaatgcggg
     2881 ttgctgatgc cgttgctcag tatatgcggg tgcccatggg ggccgggaac tcagtcccgt
     2941 gggatccgct ggtggcaccg tatgttattg agccgatgaa ctgcctggcc tcgcgtgaat
     3001 acgacgcagt gatatttgtt ggcccggcac gaaccggcaa gactatcggc ctgattgacg
     3061 gctgggtgat ttacaacgtg atttgcgatc ctgcggatat gctgatcatt cagatgacgg
     3121 aggaaaaagc ccgcgaacac tccaaaaaac gactcgccag aacgtttcgc gtcagcccgg
     3181 aagtggtcag tcgcctgagt ccgaacaaaa atgacaacaa cgtttatgac agaacattcc
     3241 ttgctggcaa ctacctgaaa atcggctggc cgtcagtcaa tatcatgtcc tcatcagatt
     3301 ataaatgcgt ggcgctgacg gattatgacc gttttccgga agatattgat ggcgaggggg
     3361 atgccttctc tcttgcctca aaacgtacca ccacatttat gtccagtggt atgacgctgg
     3421 tggagagttc ccccggcagg gatgtgaagg atgtgaaatg gcgacggact tcaccgcatg
     3481 aggctccacc aaccacgggg atactgtcgc tctataaccg tggcgatcgc cgtcgctggt
     3541 actggccctg tccacactgt ggtgagtatt ttcagccctg cggcgatgtg gttgctggtt
     3601 tccgtgatat tgccgatccc gtgctggcaa gtgaggcggc ttatattcag tgtccttcct
     3661 gttcaggacg gattatgcct gaacaaaaac gtgagctgaa cggacgtggg gtctggttgc
     3721 gggatggtga atccatcaat gcggatggta gtcgttatgg tgatccccga cgctcacgta
     3781 ttgcgtcatt ctggatggag ggtccggcag ctgcttacca gacactctcg caactcgttt
     3841 acaaactgct tactgcagaa caggaatacg agacaaccgg aagtgaagaa acactcaaga
     3901 cggttatcaa taccgactgg ggattacctt atcttccccg cgccagcatg gagcaacgaa
     3961 aaagtgaact gcttgagcag cgggcagagc cagttccttc cagcagtgtg ccggatggcg
     4021 ttaatttcct tgtggcgaca gtggatgtgc aggcgggacg tcatcgccgt tttgtggttc
     4081 aggtaacggg ctatggcagc cgtggcgaac gctggattat tgatcgttac aacatcacgc
     4141 agtcattgcg cggtgacagc gacggtgaga gccagcgaat tgatccggcc agctatccgg
     4201 aagactggga tgtcctgctg acggatgttt ttcataaaag ctggccgctg gcctccgatc
     4261 cttctcaaca aatgcgactg atggcaatgg cggtggactc cggcggtgaa gacggggtca
     4321 ctgataatgc ctataaattc tggcgtcgtt gccgtcgtga tggccttggt aaacgtattt
     4381 acctgtttaa gggcgacagc atccggcgcg caaaactgat cacccgtaca ttccctgata
     4441 acaccggacg aacgggccga cgggcgcagg ccgcaggtga tgtgccgctc tggcttcttc
     4501 agacggatgc actgaaagac cgggtgaata acgcgttatg gcgtgactct ccaggtcccg
     4561 gctatgtgca tttccctgac tggctgggga gctggtttta cgacgaactg acgtatgaag
     4621 agcggagtag tgacgggaaa tggagtaagc cgggtcgcgg tgccaacgaa gcttttgacc
     4681 tgatggtgta tgccgaggct ctggtcattc tgcatggata cgaaaagatc cgctggccgg
     4741 atgcaccggg gtgggcgagc cgggaaacct ggctggagag tgtcccggac agtaccgaac
     4801 cgtcaccctc accggaaccg gtatccacgc ctgttaaaaa acaaaaacgg aagaaaacag
     4861 taactgacga tgttaacccc tggctgactt ccggaggatg gttatgaacc agaatgatat
     4921 cgaagccatg attcagcgtt atacggaagc tgaaatggcg gtgctggacg gaaaatccgt
     4981 cacctttaat ggtcagcaga tgaccatgga aaacttatct gagatccggc agggacggca
     5041 ggagtgggag cgccgccttg cggctctgat tacacgacga cgggggcatc ccgggtaccg
     5101 gctggcgagg ttctgatggc aattcttgat gatgtgattg gcgttttttc accaggatgg
     5161 aaagcggcaa ggctgcgttc ccgtgcggtg atccaggctt atgaggccgt aaaaacgacg
     5221 cggacacaca aagcccggcg ggaaaaccga actgccgacc agttaagcca gtacggggcc
     5281 gtgtcgttac gtgagcaggc ccgttacctt gataacaacc acgatctggt tattggtgta
     5341 tttgacaagc tggaagaacg ggtggtgggg aaaaacggga ttattgtcga gccacatccg
     5401 gtattacgca atggggccat tgcccgtgat ctggcagcgg agatacgcac ccgatggagt
     5461 gaatggtctg tcagtccgga agtcaccggg cagtttaccc gtccgatgct ggaacgtctg
     5521 atgctgcgta cctggctgcg cgatggtgag gtgtttgccc agatggtttc cgggcgcata
     5581 aacagcctga cgccttctgc cggtgttcat ttctggctgg aggcgctcga gccagacttt
     5641 attcccatga ccagtgatga gagcaacagg ctgaatcagg gcgtgtttgt tgatgactgg
     5701 gggcgtcccg aaaaatatct ggtgtataaa agccgtcccg tatccggacg gcagatggaa
     5761 accaaagaag tggatgcaga gcgaatgctg catcttaaat ttgttcgccg tctgcaccag
     5821 atgcgcggga cgtctttgtt gtccggtgtg ctgatccgcc tcagtgccct gaaagagtat
     5881 gaggattctg agctgactgc agcaaggatc gccgctgctc tggggatgta catccggaaa
     5941 ggcgatgggc agagctatga accggatggt aatggcagca aggataagga acgcgagctt
     6001 accattcagc caggcattat ttacgacgat ctgaaacccg gcgaagaaat cggaatggtg
     6061 aagtcggatc gccccaatcc taaccttgaa acttttcgta atggtcagtt gcgtgccgtg
     6121 gcggcgggca gtcgtctgaa ttttttccag tacagcgcgc aactataacg gcacttacag
     6181 cgcccagcgt caggagctgg ttgaatccac tgatggctac ctgatcctgc aggactggtt
     6241 tattggtgcc gtcacccgcc cgatgtatcg tgcctggctg aaacaggctg tggcatccgg
     6301 tgttatcagg ctaccccgcg atcttgaccg ttcttcactg tataccgcgg tgtattccgg
     6361 accggtgatg ccgtggattg accctgttaa ggaggctgag gcctggaaaa ttcagattcg
     6421 tggtggagca gcgacagaat cagactgggt acgtgcaggt ggtcgtaatc cggatgatgt
     6481 caaacgtcgg cgcaaggccg aaattgatga aaaccgcaag ctggatctgg tatttgatac
     6541 cgatccggcc agtgataaag gaggcagcag tgccgcaacg aaacgacagg agccgcagca
     6601 caccgacgac cagtccgaag aataattcct ggttcaggat gcaggctggt caccagagtg
     6661 acgcggatat ttatatttat gacgagattg gtttctgggg tgttacagcg aagcagttta
     6721 tcagtgatct gaatgcactg ggcgatatca cccacattaa tctcaatatc aattcaccgg
     6781 gtggcgatgt ctttgaaggc atcgccattt ttaatgcact gaaaacacat ggtgcgtcca
     6841 ttaccgttta tgtcgacggt gtggcggcgt caatggcgtc ggtcattgcg atggtgggaa
     6901 aaccggtcat tatgccggaa aactccttca tgatgattca taaaccattt ggctttacgg
     6961 gcggtgatgc ggaggacatg cgcacctatg ccgacctgct cgataaagtt gaggcggttc
     7021 tgttacccgc ttatgcacag aaaaccggga aaaccaccga tgaaattgct gccatgctgg
     7081 cggatgagac ctggatgtcc ggtgccgaat gtctggcaca tggatttgct gatcaggtga
     7141 cgccagccgt taaggcaatg gcatgtattc agtcaaaacg tacagaggaa tttaaaaaga
     7201 tgccggaatc cattcgaaac atgattactc cgccacgcaa cagtgctcca cgcgtacagg
     7261 atgatggacc tgcagcctcc cggacgccag tgcaggcagc agcacctgtg gtggatgaaa
     7321 acagtatccg tgcgcaggta ctggcagagc aaaaagcgcg tgtaaacggt attaatgatc
     7381 tgtttgccat gtttggcggg cgttatcaga cgctgcaggc tcagtgtctt gccgatcctg
     7441 aatgttcgct ggagcaggcc cgcgaaaagc tgttgaacga gatggggcgc gagtccacgc
     7501 catctaataa aaataccccg gctcatattt atgccggaaa cggtaatttt gtgggggatg
     7561 ggatccgcca ggcgctgatg gcgcgtgccg gatttgaaaa aaccgaacgt gataatgtct
     7621 acaacgggat gaccctgcgt gaatatgccc gtatgtcact gactgaacgg ggtattgggg
     7681 tttccagtta taacccgatg cagatggtcg gtgcggcgtt cacacacagt acgtctgact
     7741 tcggtaatat tctgctggat gttgcgaaca aagccattct gcagggctgg gaagatgccc
     7801 ctgaaaccta tgaacagtgg acgcggaaag gtcagttgtc tgattttaaa attgcccatc
     7861 gtgtgggtat ggggggcttc agtgctctgc gtcaggtgcg tgaaggggcg gaatataaat
     7921 acgtcaccac cggagataaa caggccacta ttgcactggc gacctatggc gagctgttca
     7981 gtatcacccg tcaggccatt atcaatgatg atctgaatat gctgaccgat gtcccgatga
     8041 aactgggccg tgcggcgaaa tccactattg ccgatctggt ttatgccatt ctgacgtcta
     8101 acccgaaaat ctccacagat aatgtaagtc tgttcgataa agcgaaacat gcaaacgtac
     8161 tggagagcgc tgcaatggac gtggcatcgc tggataaagc ccgccagttg atgcgcgttc
     8221 agaaagaggg ggagcgtcat ctgaatattc gtcctgcgtt cgtactggta ccgacggcga
     8281 tggagtctgt tgctaaccag gtcattcgct cctcaagtgt caagggggct gacattaacg
     8341 ccggtattat taacccggtg aaagattttg cgaccgttat tgcagagcct cgtcttgatg
     8401 ataacagcca gaccaccttc tacctggctg cgtcaaaagg ctccgatacg attgaagtgg
     8461 cttatctcaa cggtgtggat acgccatata ttgatcagat ggagggcttc agtgtggatg
     8521 gcgtgacaac gaaagtgcgt attgacgccg gtgtcgcgcc agttgatcac cgcggtctgg
     8581 tgaaatgtac ggcgtaaacg tcgcagacaa caactctgat ggcccgtaag ggcttttttt
     8641 gtacctgaaa tcagcccctg aacggggctg tgcggagaca gttatggcaa agaattttgt
     8701 agaagaagga aaaacggtgg cgattgttgc cagtgcagcc atcagcagcg gagatctggt
     8761 gcaggtgggc gatgtttttg cggtggcgct gaccgatatt ccacagggtg aaacaggcga
     8821 cggcatgacc gaaggtgtgt ttatgctgcc taagctgaaa acggatgaca tgaaaacggg
     8881 taagaaggtt tatctgaagt caggaaaagt tcagctgact aacagcggct ctgatccgct
     8941 ggtcggggtt gtctgggcag atgccggaac cagtgcagaa gaagtgccgg taaaactcaa
     9001 tgtctgatcc cttttcccgg ctggcagcgc gtatggatgc tatcacggtc agaaagatgg
     9061 gaaagacagc ctcgattaat gatgtcgata tgactgtgat cccgggagaa acactggcag
     9121 agctgaatgc tctgtccgga cctgcggtct ctctggtggt gttttcttcg ggataccgcc
     9181 cacggcgcgg ggatcgcgtt gtttatgacg gacaacaatg gacggtcaca cggcatgaac
     9241 gctttaacgg taagccaatg atctttattg agtaaagagg tgtgggatga aggggcttga
     9301 gaatgccatc cgcaatctga acagccttga tacccgtatg gtgccacagg ccagcgcatg
     9361 ggcgataaac cgtgtggcac agaaagcggg ctcggttgcc acccggcagg ttgccgggaa
     9421 taccgttgcg ggagataacc aggtgaaagg gatccccctg aaactggttc gtcagcgtgt
     9481 ccgggtgttt aaagccagtc cgtcaggaaa aatgacggcc aggatccgcg ttaaccgggg
     9541 caatctgccc gccattaagc tggggacagc ccgggtcaga ctggcccagc gtggtggaaa
     9601 actgcagtac cgtggcagtg tgctgaaggt gggtaaatat cttttccggg atgcgtttat
     9661 tcagcaactg gcgaatggtc gctggcatgt gatgcggcgt attgatggca aaaatcgtta
     9721 ccccattgat gtggtgaaaa tcccgctgtc cggaccgctg acacaggcat ttgaagatgc
     9781 ccgcgaccgc atcattgctg cggaaatgcc gaaacagctg gggtatgcac tgaaacaaca
     9841 actgaggtta tggctgaccc gatgaaccga catacacaaa tccgccaggt cgtactggca
     9901 cgccttcggg aacagtgtgg agacagcgcc acgttttttg acgggcttcc ggcatttgtt
     9961 gatgcgcagg aactgcctgc cgtggcggtg tggctgagtg atgctcagta caccggaaaa
    10021 atgacggatg aagatgactg gcaggctgtt ctgcatattg ctgtcttcat ccgggcacag
    10081 gcaccggatt cagagctgga tatgtggatg gagagcacca ttttcccggc cctgaatgat
    10141 gtatcggcac tttccggact catcgacacc ctgatcccac tcggttttaa ctatcaacgt
    10201 gataatgaga tggccacctg ggcgatggcg gaaatcacgt accagatcac gtacacgaat
    10261 taaggaggtg gtaatgacca cgccaaatcc actggcaaag acgaaaggtg cggggacgac
    10321 gttctggatg tataccggca acggcgatgc gtttgcgaac cctttgtcgg acactgactg
    10381 gctgcgtctt gcgatggtga aggatctgca acctggcgaa atgaccgctg atgcagaaga
    10441 tgacacttat ctcgatgatg aagatgcaga ctggaaaacg acaacccagg ggcagaaatc
    10501 cgttggtgat acttcggcga cgctggcctg gcgtccgggt gacagcgggc agaaaaaact
    10561 ggttcagttg ttcgactccg gtgaagtctg cgcgtttcgt atcaaatatc ccaacggcac
    10621 tgttgatgtt ttccgcggct ggctgagctc actgggtaaa accattgcct caaaagacgt
    10681 gatgacccgc acagtgaaaa tcagcggtgt ggggcgtcca tatctggcag aggagggcac
    10741 tgaaactgtg ggcgttaccg ggctgacggt ggcaccggca tccgccagtg tcaatgtggg
    10801 agcaaccacc acgctgacct ttacagtaaa acctgacgga gccagtgaca aagcgatcag
    10861 tgtgcattcg acagatccac agacagccac tgtgaccctg aacggacttg tggccacggt
    10921 gaaaggcgtg aagcagggca gcgtcagcat tgtgggcatg accgctgacg ggaattttgt
    10981 ggctgtggct gcggtgactg tcagcgctgc aggttaacag gacgatactc atcatttgcc
    11041 ccggttatcc ggggcttttt tgcaggtgga gaacatgatg tttctgaaac agggcacgtt
    11101 taattatgaa aagcagtccg tggtgctcag tgagctgtcc gggctgcaga gaattgaata
    11161 tctggcgttt gttcagcagc gaacggcaaa gtttgatgcc gaagagggag aactgccgga
    11221 ggctgaacga cagattgctt ttctgcggat ggggatggat atcaatgcct ggctggtttc
    11281 ccgctcactg tggaatgcgg aacagtctca ggatgttgag acgctttgcg catccgttat
    11341 tacaacatgg tcgtatgatg ccctgggagc gggggcggag atggttctgt cgctgagcgg
    11401 tatgggagcc attgagaatg ccggggattt ggagcatgag gtgctgacgc cggaaaagtc
    11461 ctgacgcggg aaatgcagtt tgtcatgcgg cttgcccggg agttccggcg ggcagactgg
    11521 cggcggatgc tgtcggaaat gtcggccact gagcttggtg agtggggcga ttatttccgg
    11581 atgcagagct tcagtgatgt gtggatggat gcgcagtttg cctcgctgaa ggcattgatc
    11641 gtgagaatgg tgtccggtag cagtgatgct gcggtggctg atttcagcct tttaccggaa
    11701 gagaacggga taccggagcg aacggacgaa gaactgatgc atcttgggga aggtatttcc
    11761 ggaggtgtgc gttatggacc agatagccaa cctggtcatt gatttgggga ttgatgcggc
    11821 agagtttaaa aatgaaattc cccgtatcaa aaaccttctg aatggtgcag ccagcgatgc
    11881 agaacggtct tctgcccgta tgcagcgttt tatggagcgt cagactcagg ccgcccggca
    11941 gacaatgcag gcggcttctt cggctgcaac agccgcatcc gtccatgcgc agacggtgga
    12001 gaagagcgca caggctcatg aacgcatggc ccgcgaggtg gagcaaaccc gccagcgtat
    12061 ggaggcactg agccagaaaa tgcgcgagga acaggcgcag gccatggctc tggcggaggc
    12121 tcaggataaa gcggctgctg cgttttatcg tcagattgac agtgtgaaac aggccggtgc
    12181 ggggctgcag gaattacagc gtattcagca acagatccga caggccagaa acagtggcgg
    12241 gattggtcag caggattatc tggcgctgat ttctgaggtt acggcgaaaa cccgtgttct
    12301 tacacaggct gaggaagagg ctacccgaca gaaagtggcg tttatccgtc agcttaaaga
    12361 gcaggcaacc cgccagaatc tttcttcttc tgagttgctt cgtgctaagg ctgcccagct
    12421 gggggtaagc agtgctgcag aagtgtatat ccgcaaaatg gagcaggcag gaaaagccac
    12481 gcattcgctg ggtctgaaaa gtgcagcagc ccgtcaggag ataggcgttc tgataggtga
    12541 actggcccgc ggcaatttag gtgcgctgag gggatccgga ataacgctgg ctaaccgtgc
    12601 cgggtggata gacacactga tgtcaccgaa aggcatgatg ccaggagcgg ttattggcgg
    12661 tattgccgcg gctgtctatg gtctgggtaa agcctggtat gacggtcaga aggaggggga
    12721 agaatttaac cgccagctgt cgctgacggg gcattatgcc ggagtcactg ccgggcagct
    12781 gtggacgctc agtcgtgcta tttccgggaa tggtatcacg caacatgctg cagccggtgc
    12841 gctggctcag gtggtgggga gtggtgcatt tcgtggaaac gatatcggta tggtggcgag
    12901 agctgccgca cagatggagc gatcggttgg ccagtcggtc agcgatacca taaatcagtt
    12961 taagcggctg aaggatgatc ctgtaaatgc cgcgaaggct ctggacaatg agctgcattt
    13021 tcttactgcc actcagcttg agcagatacg cgtccttggg gagcaggggc ggtccagtga
    13081 tgctgcacgg atagccatgt ctgcactggc agaggaaacc ggtcggcgta ctgcggatat
    13141 tgataataac ctcaatgcgc ttggcagtac gctgaagtat ctgtctgatt tgtggagtcg
    13201 tttctgggat gcggccatga atattggtcg tgaagactcg ctggatgaac agattgccgc
    13261 tttacaggag aaagtgtcgc gggcgaaaag actcccctgg acggcatcat cttctcaggt
    13321 tgaatacgat cagcagcgtc ttaacgatct tcaggagaaa aaacgccaga aggatttgca
    13381 ggatgcaaaa gagcaggcag agcggaatta tcaggagcaa cagaaacgcc gtaatgctga
    13441 aaatgctgca ctgaaccgga tgaatgaaac ggaagcagca cgacatcagc gtgaaatagc
    13501 gcgtattaat gccatgcagt acgccgatca ggctgtcagg gatgcggcga tacaacgtga
    13561 aaatgaacgt tacgagaaag ccctggcatc cggtaagaaa aaaacacgcg aaacccgtaa
    13621 tgatgaggcc acccggttat tgctgcagta cagtcagcaa caggcacagg tggaaggaca
    13681 gattgctgct gccagacagt cagcaggcat tgccacggaa aggatgacag aagcgcgtaa
    13741 acagcttctg gctctgcagc agcgcatcag cgacctggac gggaaaaaac tgacggcaga
    13801 tgaaaagagt gtgctggccc gtaaagatga actgattcag gcactgacgc tgctggatgt
    13861 aaaacagcag gagcttcaga aacagacggc actcaacgag ctgaagaaaa aaacaattca
    13921 gctgaccagt caactggctg aagaagagcg cgctcagcgt cagcaacatg acctggatat
    13981 cgccacggtg ggtatgggtg atcagcagcg acagcgatat caggtacaac tgagtcttcg
    14041 ccagaaatac cagcaacagc tggagcagtt gaggcgggat agtgagcaga aagggacata
    14101 taacacggat gactacagaa aggccgagca ggcgctgacg gagagcctga accgacaact
    14161 gaatgagaat cgccgttact ggcaacagct tgaagttgta cagggtaact ggaaaaacgg
    14221 agtcctgcgt gcatttcagg attttaccgt ggatgcagat aatacggcag aaacagcaga
    14281 acaggtgttc tcgtcagcct tcagcaacat gggaaatggc ctggcaactt ttgtcactac
    14341 cggcaaactc aatttcaaat ccttcacctc ttctgtgctg tcagatatgg cgaaaatcct
    14401 ggcgcaggca accatgatga aatcgataaa agggattggc agtgtactgg gatttgatct
    14461 cagcagcctt tccctgaatg ccaatggggg gatttatcag tctgctgatt tgagtcgtta
    14521 cagtggcacg gtggttaacc gtccgacgtt ttttgctttt gcaaaaggcg cgggtgtgat
    14581 gggggaagcg ggacctgaag ccattctgcc attgcgtcgt ggtgctgacg gtaagctggg
    14641 ggttgtggcg gatattgggg gttcaggtat ggcgatgttt tccccgcagt acaacatcga
    14701 gatcaataac gatggcacga acgggcagat aggtccggct gccctgaagg cggtttatga
    14761 cctcgggaaa aaagcggcag cggactttat gcaacagcag gcccgtgatg gtggtcggtt
    14821 aagtggagca tatcggtaat ggagacgttt cactggaaag tgcgcccgga tatgaatgtg
    14881 gtatcagagc cgaaagtggt gacagtgaag ctgggcgatg gttatgaaca gcgtcgtgcg
    14941 gcgggactga ataaccagtt gtcgacttac agcgtgacga tacgtgttcg taaatgtgaa
    15001 cacccatctt taaaagcctt tctggaacgg cacggtggcg tccgtgcatt tcagtggacg
    15061 ccaccttatg actggaaacc gatcagggtg gtttgtcgta aatggtcggc aagcgtgggg
    15121 gcgctgtggg taaccataac ggcagatttt gaacaggtcg tggcatagga ggctctgatg
    15181 caggatattc cacaggaaac acatcatgag acgacacgcc tcactcagtc agcccaggcg
    15241 gtgctctggg aaatcgatct gacagaggtc ggtggtgaac gttatttttt ctgtaatgag
    15301 cagaacgaaa aaggtgagcc ggttacctgg caggggcggc agtatcaggc ataccccatt
    15361 caggggacgg gatttgaact gaacggcaag ggcagttctg cccgtccgac actgacggtt
    15421 tctaacctgc acggcatggt cacggggatg gcggaagacc tgcagagtct ggtcggcgga
    15481 acggtggtcc ggcgtaaggt ttacgcccgt tttctggatg cggtgaactt cgtcaacgga
    15541 aacag
//
LOCUS       DBFIFM010000110        15412 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.110, whole genome shotgun sequence.
ACCESSION   DBFIFM010000110 DBFIFM010000000
VERSION     DBFIFM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.110"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..343
                     /gene="mdtJ"
                     /locus_tag="N7C74_002920"
     CDS             <1..343
                     /gene="mdtJ"
                     /locus_tag="N7C74_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HGU2432753.1"
                     /translation="GLAIATEITGTLSMKWASVSEGNGGFILMLVMISLSYIFLSFAV
                     KKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLIKSGTRKAR
                     KPELEVNHGAV"
     gene            330..659
                     /gene="mdtI"
                     /locus_tag="N7C74_002921"
     CDS             330..659
                     /gene="mdtI"
                     /locus_tag="N7C74_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HGU2432754.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(698..1519)
                     /gene="ydgD"
                     /locus_tag="N7C74_002922"
     CDS             complement(698..1519)
                     /gene="ydgD"
                     /locus_tag="N7C74_002922"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HGU2432755.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1619..1702)
                     /locus_tag="N7C74_002923"
     CDS             complement(1619..1702)
                     /locus_tag="N7C74_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432756.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(1795..2103)
                     /gene="asr"
                     /locus_tag="N7C74_002924"
     CDS             complement(1795..2103)
                     /gene="asr"
                     /locus_tag="N7C74_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HGU2432757.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            2374..2478
                     /locus_tag="N7C74_002925"
     CDS             2374..2478
                     /locus_tag="N7C74_002925"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HGU2432758.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(2527..3780)
                     /gene="ynfM"
                     /locus_tag="N7C74_002926"
     CDS             complement(2527..3780)
                     /gene="ynfM"
                     /locus_tag="N7C74_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2432759.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKSLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            3887..4780
                     /gene="ynfL"
                     /locus_tag="N7C74_002927"
     CDS             3887..4780
                     /gene="ynfL"
                     /locus_tag="N7C74_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2432760.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            4915..6135
                     /gene="mlc"
                     /locus_tag="N7C74_002928"
                     /gene_synonym="dgsA"
     CDS             4915..6135
                     /gene="mlc"
                     /locus_tag="N7C74_002928"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HGU2432761.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            6260..6955
                     /gene="bioD"
                     /locus_tag="N7C74_002929"
     CDS             6260..6955
                     /gene="bioD"
                     /locus_tag="N7C74_002929"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HGU2432762.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(6908..8200)
                     /gene="clcB"
                     /locus_tag="N7C74_002930"
     CDS             complement(6908..8200)
                     /gene="clcB"
                     /locus_tag="N7C74_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HGU2432763.1"
                     /translation="MHHLHTYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(8358..8972)
                     /gene="dmsD"
                     /locus_tag="N7C74_002931"
     CDS             complement(8358..8972)
                     /gene="dmsD"
                     /locus_tag="N7C74_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HGU2432764.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(9015..9869)
                     /gene="ynfH"
                     /locus_tag="N7C74_002932"
     CDS             complement(9015..9869)
                     /gene="ynfH"
                     /locus_tag="N7C74_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HGU2432765.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKTDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFVTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLSSIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(9871..10488)
                     /gene="ynfG"
                     /locus_tag="N7C74_002933"
     CDS             complement(9871..10488)
                     /gene="ynfG"
                     /locus_tag="N7C74_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HGU2432766.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(10499..12922)
                     /gene="ynfF"
                     /locus_tag="N7C74_002934"
     CDS             complement(10499..12922)
                     /gene="ynfF"
                     /locus_tag="N7C74_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HGU2432767.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLISENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTNEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(12983..15409)
                     /gene="ynfE"
                     /locus_tag="N7C74_002935"
     CDS             complement(12983..15409)
                     /gene="ynfE"
                     /locus_tag="N7C74_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HGU2432768.1"
                     /translation="MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEQVVWGACSVNCGSRCALRLHVKDNEVAWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNITRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKSREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVYNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
BASE COUNT         3792 a         3884 c         3977 g         3759 t
ORIGIN      
        1 aggtctggct attgctacag aaattaccgg tacgctgtct atgaaatggg cgagcgtcag
       61 tgagggaaat ggcggcttta ttttaatgct ggtgatgatt tctctgtcgt atatatttct
      121 ctctttcgct gttaaaaaaa tagccttagg tgtggcttat gcgctgtggg aaggtatcgg
      181 tattttattt attaccttgt ttagcgtttt gttattcgac gaaagtttat cgctgatgaa
      241 aattgccggg ttaaccaccc tggtcgccgg gatagtgttg atcaaatcag gtacccgtaa
      301 agcacgtaaa cctgaactgg aggtgaacca tggcgcagtt tgaatgggtt cacgccgcct
      361 ggctggcatt ggcaatcgtg ctggaaatcg ttgctaacgt ctttttgaaa ttttctgacg
      421 gctttcgtcg caaaatattt ggcttgctct ccctggcggc ggtgctggct gcctttagtg
      481 cgctttctca ggccgttaaa gggatcgact tgtctgtcgc ttatgcattg tggggcgggt
      541 ttggtattgc cgccacgtta gccgcaggtt ggatcttgtt tggtcaacgg ttaaatcgta
      601 aaggctggat tggcctggtc ttgctgttgg ctggaatgat catggtgaaa cttgcctgat
      661 gaagacgctg cccgcgctgt cgggcagcgt ttgaacatta tttttgcgac agttgatcca
      721 gcttgtcgcg aaaaccggta acagaaatgg cccggttatc ggcgcgccag cgatctttcg
      781 cggcaggagc cgaactttgc accccaatta attgccagcc gtcatcggta tgcaacatca
      841 gaggcgaacc gctgtcaccc ggcaaggtat cgcactgatg tgacatcacc gacgtttgcg
      901 cccagccagt cacttcacag ttttgatgac tgtacaacgt atcgagatga tcttcagggt
      961 atcctgcctg agtcacttta cgacctgccg cttttaatgc ggcggtaagc gcggctttat
     1021 ctccctcaaa taacggcaac ggcgtaatgc cagaaggggg attacgtagc acaatcaatc
     1081 cgaagtccca cggcgcggct gcgggaggta caatccaacc atccccatct gcttttaacc
     1141 gctttcccag tgtcggatca acgcggcctt ctatgtcgtg gatgtcatag cgccaaagac
     1201 ctttatttga cacaaaacgc agcgccactg ctttatcggc tttacccttt ggtggcgtca
     1261 ataaacagtg tcctgccgtt aatgccagat tgggtgcaat cagcgtcgcc gtacataaat
     1321 tgccgctggc cgtttccagt tgcccaaccg catcccacgg tgattgggtc gtgtcattca
     1381 ctggcacacg atcatcatga ccaaaaaaca gggtgctgac ctcatcgttt gccgatttgg
     1441 caacgtctgg tttatctgca aacacaaaag cagacgtcaa actaattgca cccaacacta
     1501 cagcaatggt tgtacgcata tcacactctg gtgggtaatt atgattatta aaagcaaacc
     1561 ctcataaata ctatagacgg gacgggatga aagtgggagt aaaatcagat aactacgatc
     1621 aggaaagata aaaacgggcg gcaatcagtg cgcataaaat aaggatgata agaatgaact
     1681 caaagcgata acggcccacc atactaccct ccgctaaagg cggtgctaaa cgttacccgc
     1741 ttagcaccgt aattttcagg cgcgaggggg cgcgccagca ttactgttga aaacttacgc
     1801 tgcgggttgt gcagcaggtt ttgccggttg ctgatggctg tgtttcttgg cgtgtttctt
     1861 cgctgcctgc gctttttgtt ctggggcttt ctgttcagct ttcgtatttt tatgatgctt
     1921 tttagccgcc tgcgctttct gggcaggtgc tgctttatgc tgttttttat gatgtgtagt
     1981 tttcgccggc gctgctttgg tggtcgtcgc agtcggagca ggtgtggtcg cagtctctgc
     2041 agcaaaggcg gcagaagaca gacccatagc agcggcaaca accagagcta atactttttt
     2101 cattgtcata ccctcaattt gttttttcat ttaaccccac tgcggggccg ttgaaagaac
     2161 tatatccctg ggaatggcag acttccgtga gtggttggtt tcagcgtgta acgatatgta
     2221 caaacgctga ataaattaca gcgttgataa taacgtattg tgacttaagg aaaatttagc
     2281 taccaataat agtagtcttg atcgggttaa ctattttact acttacaaag cggtagaata
     2341 actgcgcatc agtaataaat gacacacagc aaaatgaatc cgtttatttg ggtacttata
     2401 atcctgatga cgctagacgc gctgcgggaa ttggctggcg cttcgtctat tttaggatgg
     2461 ctattaacgt tggtttgagc aggcaataag tccggacggg tatttaccgc agtccggact
     2521 tatttttcag gcgtgcagac gacgatgcaa acgcgtcccg accagcagag caatgaccag
     2581 catcagagca ataaatgcgc cgacgccgtt ccagccatag ttatgccaga aaacaccacc
     2641 cagcgtcccg gcaatactcg accccagata gtaactgaac agatacagcg aggaggcctg
     2701 gcctttagcg cgttttgcgc gggggccgat ccagctgctg gcaactgagt gggctgcgaa
     2761 gaatcctgct gagaagagta acattccggc aaagatcagc cacagcgagc tgaataaggt
     2821 catcagtaaa ccaaacagca taacccccgt cgaaaacaac atcactggac cacgcccata
     2881 gcgggtggtc atggttccgg ctttgggtga gctccatgta ccggtcaaat aagccagcga
     2941 taataagcca accacggcct gactgacatg ccagggggag agcatcaacc gatagccgat
     3001 gtaattaaac agcgtgacga acgaccccat cagcaaaaag ccttctgcga acaataacgg
     3061 taatccccgg tcacgccagt gcagacgaaa gttgataaac aacgacttag gtcgcagcga
     3121 agtcgggcga aaatggcgtg attccgggag gattttccag aacatcaacg ccgaggctag
     3181 cgcgaaacaa ccgatagccg ccagagcaat tcgccagttg aaaaagtccg tgaagacacc
     3241 actaattaag cgtccgctca tgccgccaat tgagttgccg ctgatataca accccatcga
     3301 aaaggccacg aaactggggt ggatttcctc gctaagataa gtcatgccaa cagctgccac
     3361 gccacttaac gaaagcccaa tcaaggcgcg cataatcaaa atgccgtgcc agctggtcat
     3421 cattgtcgaa agtaacgtac aaatggaggc caatagtagc gccgttacca tcactggttt
     3481 gcgaccaatg gcatcggata gggggccagt aaacagcaaa ccaatagcca acatcgccgt
     3541 ggaaatggac agtgaaatac tactgttcgc gggcgttaag ccaaactcct gcgaaagcac
     3601 cggaaggata ggttgcacac aatagagaag tgcaaatgtt gccagtccgg cagagaacag
     3661 cgccagggtg acgcgcataa attgcggcgt accgcgttta ataaattgat ttggctgaga
     3721 aatgctttgc ttgtcagtgt cgcttgccgg agcgccatca acagttgtag tacggctcac
     3781 ttgaaatcct tgctaaatat gcctgtggat caggcttata cataggttag gaaaatcgaa
     3841 ttgttctgtc taatatatta ataatctcaa ataagatgtt ttaaatatga atattgaact
     3901 tcgtcatctg cgttactttg ttgctgttgc ggaagagctg catttcgggc gcgccgctgc
     3961 ccgcctgaat atttcgcaac cgccgctaag tcagcagatt caggcgctgg agcaacaaat
     4021 tggtgcccga ctgctggcac gaaccaatcg cagtgtattg ctgacggcag caggaaaaca
     4081 gtttcttgca gatagtcggc aaatcctgtc tatggtggat gacgctgccg ctcgcgctga
     4141 aagactgcat cagggtgaag cgggggagtt gcgcattggt tttacttcgt cggctccttt
     4201 tattcgggcg gtgtccgata cgttatcgct gtttcgccga gattatcccg atgtccattt
     4261 acaaacccgc gaaatgaaca ctcgcgagca aatcgctccg ctcattgaag gaacgctgga
     4321 tatgggattg ctgcgtaaca cagcgttacc ggagacgctt gaacacgcag tcatcgtcca
     4381 tgaaccgctt atggcgatga tcccgcacga tcatcccctg gcaaataacc cgaatgtaac
     4441 gctggctgaa ctggcgaaag aaccctttgt cttttttgat ccgcacgtcg ggacagggct
     4501 gtatgacgat attctcgggc tgatgcgacg ttaccatttg acgcccgtca tcactcagga
     4561 ggtgggcgag gcaatgacca tcatcggtct ggtttccgcc ggtctgggtg tttcaatttt
     4621 gcctgcgtca tttaaacgtg ttcagctcaa cgaaatgcgc tgggtgccga ttgctgaaga
     4681 ggatgcggtt tctgaaatgt ggttggtctg gccgaaacat catgaacaaa gtccagctgc
     4741 gcgtaacttt cgtattcatc tgctgaatgc tctcaggtga gggaaatttc agcgaaaaag
     4801 cccgaaaaat gtgctgttaa tcacatgcct aagtaaaaat ttgacgacac gtattgaagt
     4861 gcttcaccat agcctacaga ttatttcgga gcgcgaaaat atagggagta tgcggtggtt
     4921 gctgaaaacc agcctgggca cattgatcaa ataaagcaga ccaacgcggg cgcggtttat
     4981 cgcctgattg atcagcttgg tccagtctcg cgtatcgatc tttcccgtct ggcgcagctg
     5041 gctcctgcca gtatcactaa aattgtccgt gagatgctcg aagcacacct ggtgcaagag
     5101 ctggaaatca aagaagcggg gaaccgtggc cgtccggcgg tggggctggt ggttgaaact
     5161 gaagcctggc actatctttc tctgcgcatt agtcgcgggg agattttcct tgctctgcgc
     5221 gatctgagca gcaaactggt ggtggaagag tcgcaggaac tggcgttaaa agatgactcg
     5281 ccattgctgg atcgtatcat ttcccatatc gatcagtttt ttatccgcca ccagaaaaaa
     5341 cttgagcgtc taacttcgat tgccataacc ctgccgggaa ttattgatac ggaaaatggc
     5401 attgtacatc gcatgccgtt ctacgaggat gtaaaagaga tgccgctcgg cgaggctctg
     5461 gagcagcata ccggcgttcc ggtttatatt cagcatgata tcagcgcatg gacgatggca
     5521 gaggccttgt ttggtgcctc acgcggggcg cgcgatgtga ttcaggtggt tatcgatcac
     5581 aacgtggggg cgggcgtcat taccgatggt catctgctac acgcaggcag cagtagcctc
     5641 gtggaaatag gtcacacaca ggtagaccca tatggtaaac gctgttattg cgggaaccac
     5701 ggctgcctcg aaaccatcgc cagtgtggac agtattcttg agctggcaca gctgcgtctc
     5761 aatcaatcca tgagctcgat gttacatggg caaccgttaa ccgtggactc attgtgtcag
     5821 gcggcattgc gcggcgatct gctggcaaaa gacatcatta ccggggtggg cgcgcatgta
     5881 gggcgcattc ttgccatcat ggtgaattta tttaacccac aaaaaatact gattggctca
     5941 ccgttaagta aagcggcaga tatcctcttc ccggtcatct cagatagcat ccgtcagcag
     6001 gcccttcctg cgtatagtca gcacattagc gttgagagta ctcagttttc taaccagggc
     6061 acgatggcag gggctgcgct ggtaaaagac gcgatgtata acggttcttt gttgattcgt
     6121 ctgttgcagg gttaacattt tttaactgtt ctaccaaaat ttgcgctatc tcaatttggg
     6181 ccaggaaagc ataacttaga ctttcaaggt taattatttt cctggtttat atttgtgaag
     6241 cataacggtg gagttagtga tgctgaagcg tttctttatt accggtacag acacttctgt
     6301 agggaaaacg gtggtttccc gcgcattgct acaagcgtta gcctcccagg gaaaaacggt
     6361 tgcgggatat aaacccgtag cgaaggggag caaagagaca cccgaagggc tgcgtaataa
     6421 agatgccctg gtgttgcaga gtgtttcaac catcgaactg ccttatgaag cagttaatcc
     6481 tatcgcgtta agcgaagaag aaagtagcgt ggcgcacagt tgcccaatca attacaccct
     6541 catttcaaac ggcctggcaa acctgaccga aaaagtcgat catgtcgtgg tagaagggac
     6601 tggcggctgg cgtagtctga tgaatgattt gcgtccactc tctgaatggg tggtgcagga
     6661 acaactgccg gtgttgatgg ttgtcggtat tcaggaaggt tgcattaacc atgcactgct
     6721 aacagctcag gcgattgcca acgacgggct gccgctcatt ggctgggtgg ctaaccgaat
     6781 caacccagga ctggcgcatt atgcggaaat cattgatgtg ctcggtaaaa aacttccggc
     6841 accgctcatt ggtgaactgc cttatctgcc gcgcgccgaa cagcgtgaac tggggcaata
     6901 catccgctta gctatgctgc gcagtgtgct ggcggtagat agagtcacgg tgtaacgtcc
     6961 gcgagattac cgacgcaatt acgcaggcaa tcaataaacc ggggagtagc tgatactccc
     7021 cggtcatttc acatatcatc aacgtcgaca taatcggcgc gtgcgtggtt gccgccaaca
     7081 gtgtcgccat cccggtcaat ccgagtaaaa gcgtaatttc ttcgccatca gggaaccata
     7141 atcccagact acgaccatac aacatgccaa tggcaagacc gataaatagc gtcggtgtaa
     7201 agacaccacc gggtgcgccg gaaccgctac tcgccagcac ggcacacagt ttacaaagga
     7261 agatcccggc aatgatcatt aacagtggcg gggcggttaa aaaggattgt acggtgctat
     7321 agccgttgcc ccacactgca ggtgtgaaca gggaaagcag acccacgatc aacccgccta
     7381 gtgccagttg ccagggcggc gcaagtttga gactcacaaa tccacgatga caggcgttca
     7441 ttaacgttaa caacagtggt ccgcacagac ctgccagcac acctgtactg ataatcagcg
     7501 catagtcacg agcctgaacc gtcactgaga gttgtacgct gtagagtaac gcgtcgctat
     7561 gattaatcag attgctaacc agcaatgcca cgacggcgga aataatcacc gggccgagag
     7621 aggccaacat catagtgcca aacagcactt cggctataaa taaactgcca gcaagcgggg
     7681 cacgataggc cgcagccatt cccgccgcgg ccccacaggc gatccataat ttccactcct
     7741 ggcgtggcgt aaaacgttgg gcaaaacagg aggcggcaag ggcagctaaa agaatcatcg
     7801 caccttcgcg accaattgca ctgccgctgg ttactaccag cagagaggca agcgatttaa
     7861 ccaggcttgc tgcgtaatca aactgaccat cggtttgcaa cgcttccatg taatcggtcg
     7921 gcgcatgagg gcgttgctgg gtaaatttct gccagcccat caacaacaaa cccgccgcca
     7981 gtccgccgag cgccggagtt agcagccgtc gccaggggga aaggtttgtc gctgcattga
     8041 ccagactgcc ggagtcatta ttgaggaaca accactccag tagcagcatc gcatgacgaa
     8101 acccggcaac ggcaaaggcc gcgagaatac cgacgactgt tgcgataagc agacggcgga
     8161 acatagtgcg cagatccgga taggtgtgta gatgatgcat gagcagaaat gggcaatgaa
     8221 acacgggcga ctattttgcg gcaaaagagg ccagctgcaa aagcagtgcg tgctttaaga
     8281 gacaaaaaac gtgacactgt aacgtattgt tgccgaatcg cgcgtaatcg tcaccatccg
     8341 gcaatattac ggtgatccta tcgaaagagt ggtttaaccg cgacaggaat taacagttgt
     8401 gattgccatt gcgccagcgt taagcgagcc agttcaccca gtgcacggta gaaagggtgt
     8461 tctgcttttt cgataaaaac gtcaagaaaa cgtgttgacc agggaaaaag gtgccatgcc
     8521 agcaattctt cgcattctgt ctggcgacca ttctccgcca accacgcagc catcaacagc
     8581 agtgaaccaa aatgatcttc cggttcgttt tgcttcattt caaactgaat gcctttctcg
     8641 cgcatccact gacgaagtgc caatgttgaa tcgccaaaca gcacagattc gcgatccagc
     8701 cagaccgacc cccacggcgg agacggcagt gcccacgggc cgacaaacaa acgctgccag
     8761 gcctgggcgt gagtctcttc acactgtgtc tgaaaagccg tcaccagcgg cgctaatgac
     8821 gcttctggta gaggccactg agtttcccag ccgtcactgg tgagtaccgc aaccagaggt
     8881 gcggcttcag cactctctgg agcgtaataa aacagcgcgc ccaacacgcg cgccgcgaca
     8941 gaaaaattat cttgctgtga aaaatgggtc attcctaaca tcctgaaatg tgcgggttgc
     9001 cccgcaccaa tttgttaacc tgcaatagcc attcctaccg tcatgtgcag gccataaaag
     9061 agcacacgcc cgatcatctc accgccaaga atgaggatta accccagaat aagtccggca
     9121 acgtgaggtt cacggcgacg tatgagcgga caaagccagc atcctagacc tgcgcataac
     9181 aataccacac gccagacctg taaggaggcg taatctggta ccagtgcgct ggcttgttgc
     9241 acggatgagt gaatagacga cagagacaaa ccttgcagca caatcacccc ggcgcaagcg
     9301 atcagcgcca gaacgctaat gatggcaaat ggtgtggtat taaatgtaac gcgggctgcc
     9361 cgcagaattg cggcagcaag tattggacca ctcaacaata ccgtcacgaa gaaagccagc
     9421 gtagtgtaac cgttatgcca ggttggcaca gtgtcgattt gatacacgca ggtcatcatc
     9481 cagacgaaaa tgacgccgag cgccatgctg acaagtaacc agagtttccc caacgcttgc
     9541 ggcattttac cgatgaccgc caccagccac catagtccgc caacggcaaa aaatatggaa
     9601 ccggcagcaa tctcgttact cagaccagaa gctccgattc gattcagcga gttaaacgcc
     9661 cgcagtggcg atcccaggtg catgacagag gcaataaacc cgatgcccat caacagccag
     9721 aggaaaaaca tgccacgaac aatgtgctga cggtctgcgt cagttttggc ggcgaaccag
     9781 ccaattccgc taacaatcag cgcgcctact acgcattgac ccaatacggt aaagataacc
     9841 agtggccatt catgccatcc atttcccatt ttacacttcc tccggattag ccagataacc
     9901 agtggtatcg ccggtcgggc ggctgttggc gttaggtttg ataacgatat tgggttttgt
     9961 gaaatgcgcg cgcggcagtg gcgcgacggc agcaagagtg ccgtgcttct ggcgcagctc
    10021 ttcaattgga ccgaactcaa gtgcacgcag cgggcaggat tcgacacata tgggttgttt
    10081 cccctcggcg acgcgcgaat aacaaccatc gcacttcgtc atgtgccctt tttcagcatt
    10141 gtactgtggc gcgccgtacg gacaagccat gtggcagtag cggcagccga tacaaacatc
    10201 ttcatcaacc accacaaagc catcttcacg cttatgcatt gcgccgctgg ggcagacttt
    10261 tgtacatgcg gggtcatcgc aatggttaca ggagatggag agataataag cgaacacgtt
    10321 ctggtgccag acgccattat cttcctgcca gtcgccgcca gcatattcat aaatacggcg
    10381 gaaactgact tccgggccaa gatctttaaa atctttgcac gccagttcgc aggttttaca
    10441 gccagtacag cggctggaat cgataaaaaa tccatattgt gtggtcatgg gctactcctt
    10501 aaaccttttc gatctggaca agattgctgt gcgacgggtt tccctttgcc agcggtgaag
    10561 ggcggtgaga ggtcagaata ttgatactgc cgccgtgatc gacccggtca ccaaacatat
    10621 ccgctttaag ccacgcacct tgcccgatgg cggtaacgcc aggcagaata cgcggagtca
    10681 cttttgcggc aatcagcatt tctccattat tgttaaatac ccgcacggta tcgccatgac
    10741 ggataccgcg agcctgagca tcaatggggt tgatccacac ctcttgtggg caggcctgct
    10801 gtaacacatc aatattgccg tagctggagt gggtacgcgc tttgtagtgg aagcccgtta
    10861 actgcagtgg ataggttttc cgcaggggat cgtcccagcc atcaaaacct ggggtatacg
    10921 caggaagggg atgaataatt tcatcttttt tcaattccca ggtatctgca atcttcgcca
    10981 gtcgttcaga ataaatttcg attttccccg aaggtgtttt caacgggttt gcctgtggat
    11041 cttcacggaa tgcgcggaaa gcgacgtagt gttcttccgg gcattttttc ttaaagatcc
    11101 cggtcgtttt catctcctcg tagtcgggca tctcagggtt acgttccttc gttttcgcat
    11161 ggagatattt gatccattca tgctgactgc gaccttcagt aaaggtttga taaacgtccg
    11221 gtcctaagcg tttggcgact tcactcagca tccagtagat gggtttgcgt tcaaattttg
    11281 ctgaggttgc gggttgggcg aggatcacat agcccatatt ccctgcagat tcatgagaga
    11341 taaggtcttc ttgctctgtt ggcatcaggt cgggcaacag gatatcgcaa tacttagccg
    11401 aggccgtcat gaagtggtca atgccaacaa tcatctcgca cttgctgtca tcctgaagca
    11461 cctcattggt gtgattgatg tcgccatgtt gattgatcaa tgtgttactg gcgtagcacc
    11521 ataaaaactt gatggggaca tccagttttt cttttccacg aacaccatca cgggtcgcgg
    11581 tcatttccgt accatggtcg atggcatctg tccatgtaaa gacggaaatc tgcgttttaa
    11641 caggattctc gagcatcggg aaccattcta cccccagatc ccagctacct tcgcgtacac
    11701 ctgagttgcc gccgtttatg ccgacgttac cggtgagaac ggaaagcatg gcaatagcgc
    11761 gggatgtttg ctcgccgttg gaatgtcgtt gtggccccca accctgacaa atataagcag
    11821 gttttgctga accgatctct cgtgccaact ggataatttt ttctgccggg atgctggtga
    11881 tttttgctgc ccattccggc gttttagcta tgccgtcagg cccttcgccc agaatatagg
    11941 ctttataatg cgcgttacgt ggtgcgttgg cgggcagcgt tttttcatcg taaccaacac
    12001 aatatttgtc gagaaatggc tgatcgacca tgttttcact aatcagtacc caggcaatcg
    12061 cacaggccag tgcgccatcg gtgccagggc gaatgggcag ccattcatct tcacgcccgg
    12121 cagctgtgtc gttataacgt ggatcgatga cgatcatgcg tgcgtttgaa cgttcgcggg
    12181 cttgctcgac gtagtaagtg acaccaccac cgctcatccg cgtttctgcc gggttatttc
    12241 cgaacataac gaccagtttc gtattggcga tatcatccgg gctgttgcca tcattggcac
    12301 cgaacatata actcattgcg gcactgatct gtgcggtact gtagctgcca tagcgactga
    12361 gaaaaccacc gcaagagttc atcagacggt acgggacgtt tgagttggtg atgtttccgc
    12421 catctacgcc tgttccgtac aggacatgta cagcctcatt gccgtaatct ttcaggatcc
    12481 gccgaagatt atcactgatg gtatccaggg cttcgtccca acttatccgt tcaaatttac
    12541 cttcaccgcg cttgccgacg cgcttcatgg gatatttcaa cctatcagga tgattcatcc
    12601 gtcggcggat agagcgcccg cgtaaacacg cacgaacctg atgattaccg tagacgtcgt
    12661 cacctgtcgt atcagactcc acccagtaca cggtgtcatc tttcacatgc aaacgtaaca
    12721 gacagcggct cccgcagtta acggtgcagg aactccagac cgctttctct tctaccggtg
    12781 cctctgccgc tcggaccatt tgggaaaatg gcagagtgaa agcactgctt gccagcgcaa
    12841 gactgccaag tgcggaggtt ttcatcagac ttctacggct gatttcagcc ttcatgagcg
    12901 cctctgtggt atggattttc atcattactc acttattgct tttcaaacaa aatgtcatgc
    12961 cagaatttat ggttgtagtg ggttatattt tttcgatctc gaccagattc gtgtgctgcg
    13021 ggtttccctt cgccagtggt gaagggcgca gagtggttag cgtattcaca catccgccat
    13081 ggtcgatttt atcgccagac atattggcct cgtgccaggc tccctggccc atagcgctaa
    13141 ccccagggag aatgcgtggt gtcactttgg ctggtagccg aacttcgcca cgatggttat
    13201 acacccgcac catatcgccg ttggcaatcc cacgtttctg tgcgtctata gggttgatcc
    13261 acacctcctg acggcaggca gccttcagga catcaatgtt gccgtaggtc gagtgagtac
    13321 gggatttgta atggaaacca aacagttgca gtggaaaggt actacgttca ggggagtccc
    13381 agccttcaaa ggttgaggca taaactggca gtgggcttat cacttcatct ttttccagtt
    13441 cccaggtacg ggcaatttcc gccagcttgc tggaataaat ttcaatctta ccggaaggtg
    13501 ttttaagtgg atttgcctcg gggtcgtcac gaaatgcttt gtaggcgaca aaatgaccat
    13561 tgggatcttt acgcttatag atacccattt ttttcagttc gtcgtaagac ggtaacgccg
    13621 gatctttggc aagcattttg gcgtacagat gttgtaacca ttgttcctgc gtgcgacctt
    13681 cagtgaactt ttgatagacg tcaggtccaa gacgtttcgc gacttcactc aggatccagt
    13741 aaatcggttt gcgttcgaat ttttcgctgg tgacaggctg gaggaaaatg agatatccca
    13801 tgttaccggc gtagtcgtta ggaataatat cttcctgctc aacagtcatc aggtctggca
    13861 gcagaatgtc ggcatatttt gccgatgagg tcataaagtt ttcgatgacc acaatcattt
    13921 cgcatttcga ttcgtcctgc agaatttcat gcgttttgtt gatgtcagaa tgctgattaa
    13981 ccagggtatt tcccgcgtag ttccagatga acttaatggg cacatccagt ttatctttgc
    14041 cccggacacc gtcgcggatt gccgtcattt gtgggccatg atcgatagca tctgtccagc
    14101 tgaagcagga aattgacgtt ttgaccggat tatccagcac cggcaggcgc tctatggtaa
    14161 tggtataggt cgattcacgc gcgccactat ttccgccgct gatgccgaca ttgcccgtca
    14221 gaataggcaa catagcaata gcgcgtgcgg tcagctcgcc gtttgcctgg cgttgcggcc
    14281 cccagccctg gcagatataa gcgggttttg ctgtgccaat ttcacgcgcc agtttgatga
    14341 tacggtccac cgggataccg gtaatttgcg aagcccactg cggcgttttc gctgttttat
    14401 cgtcaccttc accaagaata taggctttat agtgaccatt tttgggtgca tctgcgggta
    14461 aggttttttc gtcatagccg acgcagtatt tatcgagaaa aggttgatca acgagatttt
    14521 cgttaatcaa tacccaggca atacccgcaa ccagcgcggc atcggtgccc gggcgaatag
    14581 gaagccattc atcttcacga ccagcagccg tatcggtata tcgcggatcg ataacaatca
    14641 tttttgcgtt cgatttttcg cgggattttt caagaagata agtgatgcca ccgccgctca
    14701 tgcgggtttc tgccgggttg ttaccaaaca tcacgaccag cttgctgttt tcaatatccg
    14761 tggtgctgtt gccatcatta ctgccgtagg tgtagggcat ggcacaggaa atttgcgcag
    14821 tgctgtagga gccatactgg ttgagtgaac cgccgtagca gttcatcagg cgtttgaccg
    14881 ccgaggctga tggcgaagag cgggttatat tgccgccaac gatccccgaa gagtactgaa
    14941 tatatacagc ctcattgcca tattgttcga cggttttctt caggctactg gcgatagtat
    15001 ccagggcttc atcccagcta atccgttcga atttgccttc accgcgttta cccacgcgtt
    15061 tcattgggta attcaagcga tcgggatgat taatacgccg gcggatggag cgaccgcgca
    15121 aacaggcgcg tacctgatgg ttgccatact catcgctgcc ggtgttgtca gtttccaccc
    15181 aggccacttc attatcttta acatgtagac gaagtgcaca gcggctacca cagttgacgg
    15241 aacaggcacc ccagaccact tgttcgctgg cctgttgtac tgctgccgct gcactgcgca
    15301 gggtgaacgg caaagaaaaa ccgcctgcag ccagcgccag agaacctatc gcggtagatt
    15361 taacgagtgt tctgcggctg atgcccacca ttcgttcatt tttggacata ac
//
LOCUS       DBFIFM010000111        15311 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.111, whole genome shotgun sequence.
ACCESSION   DBFIFM010000111 DBFIFM010000000
VERSION     DBFIFM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.111"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..491)
                     /locus_tag="N7C74_002936"
     CDS             complement(<1..491)
                     /locus_tag="N7C74_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432769.1"
                     /translation="MLGHISKFDGNNSLIKHGVVQGNNIVDFDLLRNFNGGPGLNREN
                     FIYISNIFLNIKQRNEKNHSINMFREVSISGDIVSVKFYRNEKIECACDFMMAKDAQG
                     YIDLSELDLTSCHFKGDVISKVSFISSNLQHVTFECKEIGDCNFTTAIVDNVIFKCRR
                     LHNV"
     gene            complement(720..1640)
                     /gene="malM"
                     /locus_tag="N7C74_002937"
     CDS             complement(720..1640)
                     /gene="malM"
                     /locus_tag="N7C74_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HGU2432770.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(1791..3131)
                     /gene="lamB"
                     /locus_tag="N7C74_002938"
     CDS             complement(1791..3131)
                     /gene="lamB"
                     /locus_tag="N7C74_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HGU2432771.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEVNPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(3203..4318)
                     /gene="malK"
                     /locus_tag="N7C74_002939"
     CDS             complement(3203..4318)
                     /gene="malK"
                     /locus_tag="N7C74_002939"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HGU2432772.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4683..5873
                     /gene="malE"
                     /locus_tag="N7C74_002940"
     CDS             4683..5873
                     /gene="malE"
                     /locus_tag="N7C74_002940"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HGU2432773.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            6027..7571
                     /gene="malF"
                     /locus_tag="N7C74_002941"
     CDS             6027..7571
                     /gene="malF"
                     /locus_tag="N7C74_002941"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HGU2432774.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGREQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            7586..8476
                     /gene="malG"
                     /locus_tag="N7C74_002942"
     CDS             7586..8476
                     /gene="malG"
                     /locus_tag="N7C74_002942"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HGU2432775.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8848..10323
                     /gene="xylE"
                     /locus_tag="N7C74_002943"
     CDS             8848..10323
                     /gene="xylE"
                     /locus_tag="N7C74_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HGU2432776.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10367..10777)
                     /gene="psiE"
                     /locus_tag="N7C74_002944"
     CDS             complement(10367..10777)
                     /gene="psiE"
                     /locus_tag="N7C74_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HGU2432777.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQASKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVVTLWLCNSKRLKRE"
     gene            complement(10903..11046)
                     /locus_tag="N7C74_002945"
     CDS             complement(10903..11046)
                     /locus_tag="N7C74_002945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432778.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            11047..11325
                     /locus_tag="N7C74_002946"
     CDS             11047..11325
                     /locus_tag="N7C74_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432779.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSALPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(11372..13468)
                     /locus_tag="N7C74_002947"
     CDS             complement(11372..13468)
                     /locus_tag="N7C74_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HGU2432780.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERANTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIVRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13468..14205)
                     /locus_tag="N7C74_002948"
     CDS             complement(13468..14205)
                     /locus_tag="N7C74_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HGU2432781.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERANPPLQGNYTLWVGPPPSTVTLFGLISRPGNQP
                     FTPGRDVASYLSGQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSEMPDALNADILQTLTQRIPQ"
     gene            complement(14202..14840)
                     /locus_tag="N7C74_002949"
     CDS             complement(14202..14840)
                     /locus_tag="N7C74_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HGU2432782.1"
                     /translation="MKRPAFILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIIDGATWMRTMGWTEYQQVRYATARSVFKWDGTGTVKVGSDET
                     AVRVLDEEVSTDQARWHNRYWIDSGGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(14954..15196)
                     /gene="yjbE"
                     /locus_tag="N7C74_002950"
     CDS             complement(14954..15196)
                     /gene="yjbE"
                     /locus_tag="N7C74_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HGU2432783.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
BASE COUNT         3598 a         4011 c         3882 g         3820 t
ORIGIN      
        1 acattgtgta aacgtcgaca tttaaatatg acattatcaa ctattgcagt agtaaaattg
       61 caatccccaa tttctttaca ttcgaatgtt acatgttgta gatttgatga tatgaaagac
      121 accttcgaaa taacgtcacc tttaaaatga caacttgtta aatccaattc agacaggtcg
      181 atatacccct gcgcatcttt agccatcata aaatcacaag cgcattctat tttttcattt
      241 ctataaaatt ttacgcttac aatatcacca ctgattgaga cttcacgaaa catatttatt
      301 gaatgatttt tttcgttccg ttgttttata tttaaaaaaa tattgctgat ataaataaag
      361 ttttctcgat ttaaccctgg ccccccatta aaattacgta gtaaatcaaa atctactata
      421 ttatttcctt gcaccacacc atgttttatc aaagagttat tgccgtcaaa ctttgagata
      481 tgacctaaca taaaacctct gcattacaga aatgaaataa ctggaaaaag tagcctgagc
      541 gtccagacac taccggcgaa aggaaaagaa tctctgataa tgcattgaga taatggatat
      601 tcttagtgaa gaagaagtga gttactataa aaaatcgctc atatccctgg aatgaagcat
      661 aaaaaaaggt gcgccaggag acgcaccagt tgttgcaaaa tcagcactgt ggggcgtaat
      721 taccccttgc cttttacact gctgataaag gtggaacggg cagatgtcga tcccaggcgt
      781 tcagcttcat caagcagttt taacgcctta tcaacatcac ctttcgcgac agcgtttttg
      841 atcgcggtat taaaataact ttccgtgtcg ttgagcatcg gctcgctttt cttcaccggc
      901 accggagcgg gtgcagccac agctggtgcc gccgtgttgc ctaccgtaac cggagctgga
      961 gcggaagaac caaataaagg cccgaccaac acgctggagc tggagttcgt tttcactttc
     1021 agtttcagta agccatcggt ggtatgacga gcaaccggat cgggaatatc cgggatcgag
     1081 ttaccaacgc ctttggcata ggctttagcc gggtcgagca gttgggtcgt ctgctggaga
     1141 tctttttccg tggtaaagac cagaacataa agtttttgct gccccaacgc cggtgtcagg
     1201 cgcataacgc cttccagccg atctgcactc atcacgcctg gttcctggta ggtgaaataa
     1261 ctgctgggga agaaggctga tggggtcatg ttctgatcaa gaatcagcac gttcggcgca
     1321 aaaacgctgg tttgtttgtt cacttcgctg gtcagcgtca gggtcagttc gccaatgttt
     1381 gccgggacgc tgtacgcagc aaccggacca ctgatgccgg gaacgttcag ttgttggccg
     1441 ccggtcgcca gttgggtggt ctgggtttta gattgatcga ccggtgtcca ggtgagttgt
     1501 tgcagcgcgg cagatggaat ggctggcgcg tcgctggtgt tttgcggtac gtagttaaca
     1561 tcggcaaggc tgattccagg cgcgcttgcc agtaaccctg ctgataaaca gagggcgatg
     1621 agacttttat tcattttcat tgttttcacc tcaaaatctg gagctcagcg gtagccaggc
     1681 aatagcgcgc taaacccgat aatcagaggg gctttcgccc ctttcagata atgacaacct
     1741 gttttatgcc ggatgcggcg taaacgcctt atccggccca ggttttgcta ttaccaccag
     1801 atttccatct gggcaccgaa ggtccactcg tcgctgtcgc cacgaccgaa gctgccgccg
     1861 ttgaaatcag caggaacggc tttgccgtag ttcgggttaa ccttgctatc gccgttgtag
     1921 tcgtaacccc atttctcatc ccacttggcg taggttgcga agacacgaat agccgggcgt
     1981 gaccagatgc tgtcgccagc ctgccattgt tgtgcgaggg taattttgta ctgattgttc
     2041 ttgtcgccgg tgcgctggga ttcgacgttg tcgtagccga tttccatcac ggtgctcatg
     2101 attggcgtcc acttgtacat cgggcgaata ccgacggtcc accacttggt gccgttgtcg
     2161 ttatcccagt tgatatcctg gtacataccc acgtacatca tgtcccagtt gtcgcccatg
     2221 gagatcgcac cgtggtcgag gatacgcagc atgtgaccgt tgttgttgat attgtaggca
     2281 aatttttcgt tatcaaatgc aacgccagaa ccctgcgaca gacctttacc ctgcgaggtc
     2341 atcgagtcag tagcgtactg aacaacaaac ttgttaaagc ccttcaggac actctgagta
     2401 tgttcagcag tgaataacca gccgtctttc gatgcgccat caaccagacg atagttatca
     2461 cgcaggttgg cacgaccgta gtcgacaccc agttctaatg tgccgcccgg gttgacttcc
     2521 atctgcgcta aacgcacatc gaaaacgtcg ttcgcggttt cgttggtata gtcataaata
     2581 ttgttgctgg cgaaagagga agaaccacct gcttcagagg agcgggttgc tgccagagag
     2641 agtttaccga agccaacatc gatgttttcc agaccggcac caggaccaga aatatcccag
     2701 tagtagaagt cgatcatatg aacgtcatga cgttggtaga agcgcttacc tgcccagatg
     2761 gtggagcctg gcagccattc gatcaggttt ttaccctgca cgtttgcttc acggaaggcc
     2821 gggtcggtag cttcccagtc attctgttgc gcgacggaat aggccacgtt agtgtcgaaa
     2881 tagaagctct tatcgccctc tttccacact tcctgaccca attttaattc agcataagtt
     2941 tcacattcgt tgccaagacg gtatttactt tgagcaccgg tagtctggaa acactgttgt
     3001 tcaccgccgc tacctgtcca gccaataccg gaacgtgcat agccgtggaa atcaacagcc
     3061 attgcctgag cagacattac gcccgctgcg acggcaaccg ccagaggaag tttgcgcaga
     3121 gtaatcatca ttctatctcc tgagtcattg cttttctttt ttcacatcac ctgtgacagg
     3181 ctttgtgtgt tttgtggggt gcttaaacgc ccggctcctt atgcagtcga cgacatgcag
     3241 tgccatcctc acggaacaga tggcaacgct ctggcggcag gccgatagcg aatgtggcac
     3301 cttcttctac caacaccacg tcgttctggc ggtacaccag gttttgacga atggaaggga
     3361 tctggatatg gatttgggtt tcgttgccga gttgctcgac gacctgaact tcaccctcaa
     3421 ggatgacgtc agcgatatca ctcggcagta gatgttccgg gcgaataccc agcgacatat
     3481 tggctccaac ctggacatca cggctttcaa ctggcagcca gacttgctga cgatttggca
     3541 tcggcagctc cacctgcact tgatcgattg cggtggcggt cacttttacc ggcaggaagt
     3601 tcatctttgg cgaaccgata aatccggcga caaaacggtc tgccggatag tggtacagct
     3661 ccagcggttt cccaacctgc gccacgcgac cggcgtccag caccacgatt ttgtcggcca
     3721 gcgtcatcgc ttcgacctga tcgtgggtga cgtaaatcat tgtgcggccc aggcgtttat
     3781 gcagacggga gatttcgata cgcatttgca cacgcagtgc agcatcgagg ttggagagcg
     3841 gttcatcgag caaaaatacg cttggctcgg ccaccagcgt acggccaatc gccacacgct
     3901 gacgctgacc accggagagc gctttcggtt tgcgatccag caaatgcgcc agttgtagca
     3961 cttccgccac ctggttgacg cgttggttaa tcacctcttt ttttgcgcct gccagtttca
     4021 ggccaaatga catgttttct gctactgaca ggtggggata gagcgcgtag gactgaaaca
     4081 ccataccaac gccacgttct gctggcggag tgtcattcat ccgtttctca ccgatgaaca
     4141 ggtcgccgct ggtgatcgtc tcaagcccgg caatcatgcg cagtaaagtc gatttaccgc
     4201 agccagacgg tccgacaaac accacgaatt caccttcatg gatatcgaga ttgatatctt
     4261 tcgataccac gacctcgccc caggctttcg ttacattttg cagctgtacg ctcgccatgc
     4321 ccttctccct ttgtaacaac ctgtcatcga cagcaacatt catgatgggc tgactatgcg
     4381 tcatcaggag atggcttaaa tcctccaccc cctagctttt ttatggggga ggaggcggga
     4441 ggatgagaac gcggcttctg tgaactaaac cgaggtcatg taaggaattt cgtgatgttg
     4501 cttgcaaaaa tcgtggcgat tttatgtgcg catctccaca ttactgccaa ttctgtaaca
     4561 gagatcacac aaagcaacgg tggggcgtag gggcaaggag gatggaaaga ggttgccgta
     4621 taaagaaact agagtccgtt taggtgtttt cacgagcact tcaccaacaa ggaccataga
     4681 ttatgaaaat aaaaacaggt gcacgcatcc tcgcattatc cgcattaacg acgatgatgt
     4741 tttccgcctc ggctctcgcc aaaatcgaag aaggtaaact ggtaatctgg attaacggcg
     4801 ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat accggaatta
     4861 aagtcaccgt tgagcatccg gataaactgg aagagaaatt ccctcaggtt gcggcaactg
     4921 gcgatggccc tgacattatc ttctgggcgc acgaccgctt tggtggctac gctcaatctg
     4981 gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat ccgtttacct
     5041 gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt gaagcgttat
     5101 cgctgattta taacaaagat ctgctaccga acccgccaaa aacctgggaa gagatcccgg
     5161 cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac ctgcaagaac
     5221 cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag tatgaaaacg
     5281 gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg ggtctgacct
     5341 tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac tccatcgcag
     5401 aagctgcctt taataaaggc gaaacagcga tgactatcaa cggcccgtgg gcatggtcca
     5461 acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc aagggtcaac
     5521 catctaaacc gttcgttggc gtgctgagcg ccggtattaa cgccgccagt ccgaacaaag
     5581 agctggcgaa agagttcctc gaaaactatc tgctgactga cgaaggtctg gaagcggtta
     5641 ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag ttggcgaaag
     5701 atccacgtat tgcagccacc atggaaaacg cccagaaagg tgaaatcatg ccgaacatcc
     5761 cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc gccagcggtc
     5821 gtcagactgt cgatgaagcc ctgaaagacg cgcagactcg tatcaccaag taatgctgtg
     5881 aaatgccgga tgcggcgtga acgccttgtc cggcctacaa aaccgaaacg tatgtaggcc
     5941 tgataagacg cgtcagcgtc gcatcaggca gttgttgtcg gataaggcat gaaagcctta
     6001 tccgtcctgg aatgaggaag aaccccatgg atgtcattaa aaagaaacat tggtggcaaa
     6061 gcgacgcgct gaaatggtca gtgttaggtc tgctcggcct gctggtgggt taccttgttg
     6121 ttttaatgta cgcacaaggg gaatacctgt tcgccattac cacgctgata ttgagttcag
     6181 cggggctgta tattttcgcc aatcgtaaag cctacgcctg gcgctatgtt tacccgggaa
     6241 tggctggaat gggattattc gtcctcttcc ctctggtctg caccatcgcc attgccttca
     6301 ccaactacag cagcactaac cagctgactt ttgaacgtgc acaggaagtg ttgttagatc
     6361 gctcctggca agcaggcaaa acctataact ttggtcttta cccggcgggc gatgagtggc
     6421 aactggcgct cagcgacggc gaaaccggca aaaattacct ctccgacgct tttaaatttg
     6481 gcagagagca aaaactgcaa ctgaaagaaa cgaccgccca gcccgaaggc gaacgcgcaa
     6541 atctacgcgt gattacccag aatcgtcagg cgctgagtga cattaccgcc attctgccgg
     6601 atggcaacaa agtgatgatg agctccctgc gccagttttc tggcacgcag ccgctctaca
     6661 cactcgacgg tgacggcacg ttgacgaata atcagagcgg cgtgaaatat cgtccgaata
     6721 accaaattgg cttttaccag tccattaccg ccgacggcaa ctggggtgat gaaaagctaa
     6781 gccccggtta caccgtgacc accggctgga aaaactttac tcgcgtcttc accgacgaag
     6841 gcattcagaa accgttcctc gccattttcg tctggaccgt ggtgttctcg ctgatcactg
     6901 tctttttaac ggtggcagtc ggcatggttc tggcgtgtct ggtgcagtgg gaagcgttgc
     6961 gcggcaaagc ggtctatcgc gtcctgctga ttctgcccta cgcggtgcca tcgttcattt
     7021 caatcttgat tttcaaaggg ttgtttaacc agagcttcgg tgagatcaac atgatgttga
     7081 gcgcgctgtt tggcgtgaag cccgcctggt tcagcgatcc gaccaccgcc cgaacgatgc
     7141 tgattatcgt caatacctgg ctgggctatc cgtacatgat gatcctctgc atgggcttgc
     7201 tgaaagcgat tccggacgat ttgtatgaag cctcagcaat ggatggcgca ggtccgttcc
     7261 agaacttctt taagattacg ctgccgctgc tgattaaacc gctgacgccg ctgatgatcg
     7321 ccagcttcgc ctttaacttt aacaacttcg tgctgattca actgttaacc aacggcggcc
     7381 cggatcgtct tggcacgacc acgccagccg gttataccga cctgcttgtt aactacacct
     7441 accgcatcgc ttttgaaggc ggcgggggtc aggacttcgg tctggcagca gcaattgcca
     7501 cgctgatctt cctgctggtg ggtgcgctgg cgatagtgaa cctgaaagcc acgcgaatga
     7561 agtttgatta agggagataa caaaaatggc aatggtccaa ccgaaatcgc aaaaagcacg
     7621 tttgtttatt actcacctgc tactgctact ttttatcgcg gcgattatgt tcccgctgct
     7681 gatggtcgtc gctatctcac tgcgccaggg gaactttgcg accggcagcc tgatcccgga
     7741 gcaaatctcc tgggatcact ggaaactggc attaggtttt agcgtggaac aggctgatgg
     7801 tcgcattacg ccaccgccat tcccggtgct gctgtggctg tggaactcgg taaaggtcgc
     7861 cgggatttcc gcgattggca ttgtggcgct ctccaccacc tgcgcctacg ctttcgcccg
     7921 tatgcgcttt ccaggcaaag cgacgctgct gaaaggaatg ctgattttcc agatgttccc
     7981 ggcagtactt tcactggtcg cgttgtatgc gttgtttgat cgtctgggtg agtacattcc
     8041 attcattggc ctgaatactc acggcggcgt aattttcgcg tatctgggtg ggattgcgct
     8101 gcacgtctgg accatcaaag gctatttcga aaccatcgac agttcgctgg aagaagcggc
     8161 tgcgctggat ggtgcgacac cgtggcaggc cttccgcctt gtcctgttgc cgctgtcagt
     8221 accgattctg gcggtggtat tcatcctgtc gtttatcgct gccattactg aagttccggt
     8281 cgcgtcgctg ttactgcgtg acgtaaacag ctacaccctg gccgtgggga tgcagcaata
     8341 cctcaatccg caaaactacc tgtggggtga ctttgccgcc gctgccgtga tgtctgcatt
     8401 accgatcacc atcgtcttct tgctggctca acgctggctg gtcaacggcc tgacggcagg
     8461 tggtgtgaaa ggttaaagat gttgttctgc caatgttatg ccgctgcacc ctcaacttac
     8521 gttatcccaa cttgtgactg ttattcggcg ctccacggag cgcctttttt tctttcgtct
     8581 gcaatctgaa tcgttcgccg gttaatattt ccatcataga gcttattatt tttacgttat
     8641 ttgttttccc acttacgata attctctttc gtgctctgag tcacggcaat agtattgttt
     8701 ttatcaattt tggataatta tcacaattaa gatcacagaa aagacattac gtaaacgcat
     8761 tgtaaaaaat gataattgcc ttaactgcct gacaattcca acatcaatgc actgataaaa
     8821 gatcagaatg gtctaaggca ggtctgaatg aatacccagt ataattccag ttatatattt
     8881 tcgattacct tagtcgctac attaggtggt ttattatttg gctacgacac cgccgttatt
     8941 tccggtactg ttgagtcact caataccgtc tttgttgctc cacaaaactt aagtgaatcc
     9001 gctgccaact ccctgttagg gttttgcgtg gccagcgctc tgattggttg catcatcggc
     9061 ggtgccctcg gtggttattg cagtaaccgc ttcggtcgtc gtgattcact taagattgct
     9121 gctgtcctgt tttttatttc tggtgtaggt tctgcctggc cagaacttgg ttttacctct
     9181 ataaacccgg acaacacagt gcctgtttat ctggcaggtt atgtcccgga atttgttatt
     9241 tatcgtatta ttggcggtat tggcgttggt ttagcctcaa tgctttcacc aatgtatatt
     9301 gcggaacttg ctcctgctca tattcgcggg aaactggttt catttaacca gtttgcgatt
     9361 attttcgggc agcttttagt ttactgcgta aactatttta ttgcgcgttc cggtgatgcc
     9421 agctggctga atactgacgg ctggcgttat atgtttgcct cggaatgtat ccctgcactg
     9481 ctgttcttaa tgctgctgta taccgtgcca gaaagtcctc gctggctgat gtcacgcggc
     9541 aagcaagaac aggcggaagg tatcctgcgc aaaattatgg gcaacacgct tgcaactcag
     9601 gcagtacagg aaattaaaca ctccctggat catggccgca aaaccggtgg tcgtctgctg
     9661 atgtttggcg tgggcgtgat tgtcatcggc gttatgctct ccatcttcca gcaatttgtc
     9721 ggcatcaatg tggtgctgta ctacgcgccg gaagtgttca aaacgctggg ggccagcacg
     9781 gatatcgcgc tgttgcagac cattattgtc ggagttatca acctcacctt caccgttctg
     9841 gcaattatga cggtggataa atttggtcgt aagccactgc aaattatcgg cgcactcgga
     9901 atggcaatcg gtatgtttag cctcggtacc gcgttttaca ctcaggcacc gggtattgtg
     9961 gcgctactgt cgatgctgtt ctatgttgcc gcctttgcca tgtcctgggg tccggtatgc
    10021 tgggtactgc tgtcggaaat cttcccgaat gctattcgag gtaaagcgct ggcaatcgcg
    10081 gtggcggccc agtggctggc gaactacttc gtctcctgga ccttcccgat gatggacaaa
    10141 aactcctggc tggtggccca tttccacaac ggtttctcct actggattta cggttgtatg
    10201 ggcgttctgg cagcactgtt tatgtggaaa tttgtcccgg aaaccaaagg taaaaccctt
    10261 gaggagctgg aagccctctg ggaaccggaa acgaagaaaa cacaacaaac tgctacgctg
    10321 taatcttcct gtccagcacg ccgcgccagt tcggcgtgct gtctttttac tcccgcttca
    10381 gccgtttcga attacacagc cacagggtga ccaccagtaa caggatcgca gccgagtaga
    10441 tcaacacatc cagtggcgat ttatgatcga cgatgatcaa gcgcacaatg gcggtgatcc
    10501 caatatagac aaagtaacgt agcgggaagt gaaaaccgga ctgaaagtac ttcacaatca
    10561 gcgcgataaa ttcgaaatag agaaagtaaa ccaccagtcc ttctaccagc tcatatttgc
    10621 tggcttgttc tggcgcgaac agcacatcag ccagatgcac cgtttctttg ccgaggaaga
    10681 caaccaaaat aaggccgagg cacagcaggc caagattgag tacggtctgc aaaatggtgg
    10741 agataaactc cacgcgcgga cgagagagtg acgtcatagc agtacctcct tcacagtggc
    10801 gagcttcctg tataacgcaa aaatgtgacg gagatctata ttttttgtgt atgttttgtt
    10861 caacctggat ttggagcgcg cctccaccaa agccggatat acctacccgt aggccggata
    10921 agccgcgcct ggcggcgcat ccggcaagac aaaccgcacc aaagccggta attcaaaaat
    10981 cacccattca ggtgattccc agccaccagc aatgcgcgaa aaatcgacaa tgttcctctg
    11041 gaacaaatga aacgtaacct gataaaggtg gtaaaaatga agccttattt tgctgctttg
    11101 atgttatcag tctctgctct tcctgcttat gctggtccgc tcggtactgc tgataaagcc
    11161 gacctaccgc aaagcaatgt ctccagccca atgatggccc agtcgctcag gcaaccagac
    11221 ctgcaaccga tatcgacgga caggaagaca gaatgttttc gcctttacac accagatcgc
    11281 aaaccggggg tgaactgcgt tcctgatggc tcaacaggcc attaagaata aaggtgccag
    11341 tgattgcgaa aactggcacc tttcccatga cttagcggaa attgacgcta cggtcgctgg
    11401 tcatgtcata caactggaac ttacgcccaa gttgctgacc accgtcacgc gtcagcggcg
    11461 tccagccaat cgccgcacgg ctgcgtgtcg ggccagacga gaagagatcc aacggtacag
    11521 agacatacac gcctttggtg aagtcccctt cgccgtactc ctctttcgaa acgttggtga
    11581 tcgtggcata gccgcccacc accacgccgc tgtcaaagcg tttagcgatc tccagcgtgc
    11641 cgcctttatc ccctgccaga tactgcccga cgctggcttt aactaacaca tcctgagcga
    11701 aagatggcgt ccagtaggcg gtcagatgtc cggttttcac gctgtagtcg gtgaatttca
    11761 tcatatcctt tgcactacgc cagtcgcgct gtttaacgta gttggcatcc agaccaaacg
    11821 cccagttgct gtccagcggg cgatacaaca cttctgcccc cgcaccgcca aacatggttt
    11881 cgagataacc tccgtagacc tgaccgtaga agccgttgcc cagatgctgg aagtagttgg
    11941 cttgcaggtt attcacatag acatcgttct gcacatactc gcgcacatgg gtacgcacgc
    12001 gcggtaagtg cgagtcctgc ggcggattgg tgtagttaaa cttgtcgtag ttgttggcaa
    12061 gatttgcaaa caggctgccg gtggtcagca gatggtccgt cagccacaaa tctgccgttc
    12121 ccatcacgcc cagctgatac atgtaaaagt tttccgggcc accgaccgac tggttcagca
    12181 ccggatcgat atggaaatca aagcgcgatt tgtcgatata ccagccctgc tcggtggact
    12241 tcggaaccac tggctcgacg cgtttttgcg ccagcttcgt ttcgtggccc aacggctctc
    12301 cggcgagatg atttttcagg ctggcgacat ccgtttccgt cgtcacctgc ggcatgttaa
    12361 ggcgattttc cgtaacgcgg atcgtcttga tcccatccgg cagatcattc atcacgatcc
    12421 gattagcacg aacgatccct tcgcgcgaat cacgatattt cacctgctcg ccggtaacat
    12481 acagcgtatc gcctttcgcc tggatctgcg gatcggcaag tccggcattg tatttcaaca
    12541 gcgttaactg attcgccacc accgaatgct gcaaaatggc atcctgcggc tgcggttgat
    12601 attgcgggcg ggcgttatcg ttgtaagacg ggcgcagatc gttaaagttg gtgcgcagcg
    12661 tgacgccaaa cataaaagtg ttggcacgtt catagctaag gttaacgtcg gcccaatcgg
    12721 taacgcgata aatcgccccg acgttaaact tacttttttg ctccagctta ccggcaaaat
    12781 cctgctgata attattgcct tcatactcca gtttcaggcg cagcggttgc cagggcgtct
    12841 ggtattccac gccgccaaac agtgaggcag gaccgtggaa catctggcta ccgtcgatag
    12901 atcccgcctg tttgtagctg ttatcgcgat agcaatattt atcactggct gaacagagcg
    12961 gatttttcac attaccgctt gtgcccaaat atccccagcc caggccaagc gtaaaatcga
    13021 acggccccca ggctttgctg gcaacaagat attccgcatc aaacagcccc gtaccgccga
    13081 tatcccgcgc accaaccgcc acttgcggca gccagtaact ctcttcccac aaacgcagtt
    13141 tgagatcgaa ggctttatct ttatacgttt gatcgccaga gaacgcttcg acgctgctgt
    13201 actgccgggt gcgcacgtcg gtgtagcgca gcgttgtttc cagccacggg aagagttgca
    13261 ctgaagctga gtaataacgg tactgatcgt tatcgcgata gttcagactc aactcccctt
    13321 cccgcgccat gcgagccgtg ggcgtttgta ataatcctac gccaccgaaa tccgactgcg
    13381 acggaccaat gggcgcagga tatgtttcgc cgtagcaagc tgtgctaatg cccagcgcca
    13441 gtaagctaag cagatgtctt tttttcatta ttgaggtatc cgctgcgtca gagtctgaag
    13501 aatgtcggcg ttaagggcat caggcatctc actccagacg gagtccgcga ggccaacata
    13561 aataatgctg ccgggcatcg gctctacgtg acgcttgttc cagtaagcca ccggcgcttt
    13621 ttgtgtgcgt ccgtccgggt aaaccaccca cgcgtagcta cgatccgcac cgctaagcag
    13681 actttgcccg gagagatagc tcgccacgtc gcgaccggga gtgaatggct gattgccagg
    13741 acggctgata agcccgaaca acgtgaccgt ggacggtggt ggtccgaccc acagcgtgta
    13801 gttgccctgc aacggcgggt tagcgcgttc agcaacgcgg acgatatcgg gatcaagatt
    13861 gattttttgc ctgcccgtca ccttcaacgc ctgaatttgc tggcgtaagg cgttaatcgc
    13921 agcggcatcg tcggcgctgg aatctgcgcc ctgttctgcc agccgtgtca gcaacgcctg
    13981 ctgctgacgc aatgctgccg ccgttgccag ctcttcgcta atcaccgcac caggccacca
    14041 gctgtttgcc agccgcggtt ggcctaccag atcgattaaa tgctctgcgc ccgttagcgt
    14101 tttggcctca ctgctgccat tgctgaacac cttgacggtt cctgccgcga agaccgatga
    14161 cgctcccaca ctcaaaagca acgcgacaat agtttgttta atcattgttt tgccgccttg
    14221 atgagagtgg ttttcaccgg gaaataatcc gcgccgagat actgttccga ctggcgaatt
    14281 tgccctccgc tgtcgatcca atagcggtta tgccagcgcg cctggtcggt ggagacttct
    14341 tcgtccagca cgcgaaccgc ggtttcatcg ctgccgacct taacggtacc ggtgccatcc
    14401 catttgaaga ctgagcgtgc ggtggcgtag cgtacctgct ggtactcggt ccagcccatc
    14461 gtgcgcatcc aggttgcgcc atcaatgatt tgtgcgggtt taatcagcgg gtcggcggca
    14521 aggttattca cttcaatcag gttgtcgccg ccaagcaaag tcttcaccag acgaccatgt
    14581 tgtgtgacga gagtggcctg atcctgagtg acccattttt gttgtccgtc ttcagcgaag
    14641 gccagcacca caaacaactg cggcccacca ttaagctgca tgtactggct ggcgtagggc
    14701 atattttgaa tatcatcgtc cgtcagctgt acgcctggcg tgccgaacag actgtcccac
    14761 agtgaattgc ccagctcttt agtcgtggct gaacaggcct gtaatagcag gcagataaga
    14821 atgaatgcag gtcgcttcac gactcttctc cagaggggca gaataaccac accggagtgt
    14881 ggttatggta aatcccttca tacttctagc cacagatgca atgactcgaa ttattttggg
    14941 gatacatact ttattactgg gtactggtgg tcgtggttgt ggtggtcccg gtattagaac
    15001 catcaccgcc accggttgcc gcgagcgcga cacccaccgc cgagcttacg gtgctgacgc
    15061 tggtcgcgga ggaactcccc gccgaaaccg acgttgctgc cgaccctgcc gcttcgccca
    15121 cctgtacagg tgcagcccac gcagaagtcg ccgcaagcgc agatatggca aaaatgccat
    15181 acagaacttt tttcataaca atttcccttc attgaatgaa tggagattta cccaaaaaca
    15241 tttcgggcgg gatcgagtat acacaactaa agcatgcgaa ttacggcgcg gggaattaga
    15301 gatggggttt t
//
LOCUS       DBFIFM010000112        15272 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.112, whole genome shotgun sequence.
ACCESSION   DBFIFM010000112 DBFIFM010000000
VERSION     DBFIFM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.112"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            111..4094
                     /gene="ehaA"
                     /locus_tag="N7C74_002951"
     CDS             111..4094
                     /gene="ehaA"
                     /locus_tag="N7C74_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HGU2432784.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINATDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIH
                     VKGTSRLTYLQGNVSGEGSQVNSETFFMGVYGSYGGNQYLSVNNGGEVNARKQISLGY
                     YDQVSDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SEKKITLNHTDKDATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITQNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVELTGNNYAFSGYID
                     VASGAVAVISEDKNIGRAELDVDGKLQINANKDWVFDNDLEGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQKGGITAGQGSLVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYALGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     RWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
     gene            4236..5324
                     /gene="pdeL"
                     /locus_tag="N7C74_002952"
     CDS             4236..5324
                     /gene="pdeL"
                     /locus_tag="N7C74_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU2432785.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMAGVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(5366..6297)
                     /gene="yahB"
                     /locus_tag="N7C74_002953"
                     /pseudo
     CDS             complement(5366..6297)
                     /gene="yahB"
                     /locus_tag="N7C74_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(6389..6896)
                     /gene="yahC"
                     /locus_tag="N7C74_002954"
                     /pseudo
     CDS             complement(6389..6896)
                     /gene="yahC"
                     /locus_tag="N7C74_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
     gene            7154..7759
                     /gene="yahD"
                     /locus_tag="N7C74_002955"
     CDS             7154..7759
                     /gene="yahD"
                     /locus_tag="N7C74_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HGU2432786.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNGDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            7799..8662
                     /gene="yahE"
                     /locus_tag="N7C74_002956"
     CDS             7799..8662
                     /gene="yahE"
                     /locus_tag="N7C74_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HGU2432787.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFRLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVIDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAGKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            8652..10199
                     /gene="fdrA"
                     /locus_tag="N7C74_002957"
     CDS             8652..10199
                     /gene="fdrA"
                     /locus_tag="N7C74_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HGU2432788.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGTKEAALKAVMLSGVKQEHLDLHTLNQPLITDVRA
                     RLKPQQKYIRGLFCGGTLCDETMFAVMEKHSDVYSNIQPDPEFRLKDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            10199..11617
                     /gene="yahG"
                     /locus_tag="N7C74_002958"
     CDS             10199..11617
                     /gene="yahG"
                     /locus_tag="N7C74_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HGU2432789.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQFDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVAQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAVDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            11643..11963
                     /gene="yahH"
                     /locus_tag="N7C74_002959"
     CDS             11643..11963
                     /gene="yahH"
                     /locus_tag="N7C74_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahH"
                     /protein_id="HGU2432790.1"
                     /translation="MNALSGLRMAQESVGLISVAHQAFVTIAGCGVNALSGLQVAQEF
                     VGLISVAHQAFITIAGCGVNALSGLRMAQESVGLISVAHQAFVTIAGCGVNALSGLRM
                     AREL"
     gene            11990..12082
                     /gene="yahH"
                     /locus_tag="N7C74_002960"
                     /pseudo
     CDS             11990..12082
                     /gene="yahH"
                     /locus_tag="N7C74_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahH"
     gene            12132..13082
                     /gene="yahI"
                     /locus_tag="N7C74_002961"
     CDS             12132..13082
                     /gene="yahI"
                     /locus_tag="N7C74_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HGU2432791.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPEKQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            13092..14474
                     /gene="yahJ"
                     /locus_tag="N7C74_002962"
     CDS             13092..14474
                     /gene="yahJ"
                     /locus_tag="N7C74_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HGU2432792.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTTNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGDKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            14773..15183
                     /locus_tag="N7C74_002963"
     CDS             14773..15183
                     /locus_tag="N7C74_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HGU2432793.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
BASE COUNT         3985 a         3634 c         4153 g         3500 t
ORIGIN      
        1 atgcaaaatt tttgaatttt tatatgcgat gaacagtaac cggaatggca tttaatgcgt
       61 tgctgtttat gcaatcttgg ttttatttgg atgtattgct ggagattgtg atgaataaaa
      121 tatatcggct aaagtggaac aggtcccgta actgttggag cgtctgctcg gagctgggga
      181 gcagagtaaa aggaaaaaag tcccgggctg ttttaattag cgcgataagt ttatattcat
      241 ctctggtatt cgccgatgat gtcatcgtaa accaggataa aactattgat tttggcaaag
      301 agaaccagag catcgattac cgtattacgg tgacagacaa tgccaatctg gtaatcaatg
      361 cgacagatac ttcccgtccg cgtctgactc tcgcttctgg tggtgggttg gatattaccg
      421 gaggaaaggt aacaatcaat ggcccactta actttttgct gaaaggtacg gggttcctga
      481 atgtctccaa tgctggcagc gagttatatg ctgatgattt gtatgaatca aactcaggca
      541 tgagacacga tcgcggctat tttaatgtct ccaacggcgg caaaatccat gttaagggca
      601 ccagccgtct gacctatttg cagggaaatg tcagtggtga aggtagccag gtaaattccg
      661 aaaccttctt tatgggcgtt tacggcagtt acggtggtaa tcagtacctg tcagttaata
      721 acggcggtga agttaatgcc aggaagcaaa ttagcctggg ctattatgat caagtctccg
      781 atacaacact tgctgtttcg gaaggtggta aaatttctgc gcctactatt agtttaagca
      841 ccaactctga gttagcgtta ggggcacagg aaggaagcgc agcgaaggca gcagggatta
      901 ttgatgccga aaaaattgag tttgtgtggg caaagacatc cgagaagaaa atcaccttaa
      961 accacacgga taaagacgcg actatttccg cggatattgt cagtggcagc gagggcctgg
     1021 gctatatcaa tgcgctcaat ggcacgactt acttaaccgg tgataactct gcctttagtg
     1081 gtaaagtcaa aattgagcaa aatggcgctt tagggatcac ccaaaatata ggtacagcag
     1141 agatcaacaa ccgcgggaaa ttacacctga aggctgacga tagcatgacc tttgccaata
     1201 agatctctgg caacggtaca ataagtatcg acagtgggac ggtggagttg accggcaata
     1261 actatgcatt cagcggatat attgatgttg cttctggtgc tgtcgctgtt atttctgaag
     1321 acaagaatat cggtcgtgca gagctggatg tcgatggcaa attgcaaatt aatgccaaca
     1381 aagattgggt atttgataac gatcttgaag gtagaggcat tgttgaaata aacatgggga
     1441 atcacgaatt ctccttcgat gagtttgctt atacagactg gttccagggt tcactggcgt
     1501 tccagaacac gacatttaat ctggaaaaga atgctgagtt tctgcagaaa ggcgggatca
     1561 ctgcgggtca gggaagcctg gtaacagtgg gtaagggcgc tcactccatt agcactttgg
     1621 gattctccgg cggaaccgtt gattttggtg ccctgacagc aggtgcacag atgacagaag
     1681 ggacggtcaa cgttagtaaa acgctggatt tgcgcggcga gggtgtgatt caggtttctg
     1741 acagtgacgt tgtccgctca gtatctcgtg atattgactc tgcgttatcg ctcactgaag
     1801 tcgatgatgg taacagcacc attaagttgg ttgatgcgca aggtgcggaa gttctgggcg
     1861 atgcgggcaa tctgcaattg caggataaaa atgggcaaat cctctccagc agcgcccaac
     1921 gtgatattca gcagaatggg caaaaagcgg ccgtcggcac ttacgactat cgtctgacga
     1981 gtggggtaaa caatgacggt ctgtatattg gttacggcct gacccagctt gatttacacg
     2041 ctaccgacag cgatgctctg gtgctgagct ctaacggtaa aagcgagaat gccgccgatc
     2101 tcagcgcaaa gattaccggc agtggtgacc tggcattcag cagccagaag ggtcagaccg
     2161 tatcgctttc taacaaagac aacgactata ccggtgttac cgatctgcgc agtgggacgc
     2221 ttttgttgaa taacgataac gtgttgggta atacccatga actgcgtctg gcggcagaga
     2281 ctgaactgga catgaatggt cacagccaga ccgtgggcac gctcaatggc agcgccgatt
     2341 cactgctgag cttaaatggc ggcagtctga cggttaccaa cgggggcact tcaaccggtt
     2401 cgttaacggg gagcggagag ctgaatattc agggcggcac gctggacatc gcgggcgata
     2461 acagcaacct gacggcgaat gtgaacattg ctaattcggc taatgtcctg gtaagtcatg
     2521 cgcagggatt gggtagcgca aacgttgaga acaacggtac cctggcgttg aataatagcg
     2581 ctgaaaaaag agcggctgcg tctgtgaatt acgccctggg cggcaatctg accaacaacg
     2641 gtacgctgat gaccggaatg tcaggacagc aagctggcaa tgtgttagtg gtgaagggga
     2701 actaccacgg taataacggt caactagtaa tgaatacggt actgaatggc gatgactcag
     2761 taaccgataa attggttgtc gagggcgata ctagcggcac gactgccgtt acggtgaata
     2821 acgctggcgg tacaggtgcg aaaaccctta acggtatcga acttatccat gtagacggta
     2881 agtctgaggg cgaatttgtt caggctgggc gtatcgttgc gggggcgtat gactacactc
     2941 tcgcgcgtgg acaaggggca aatagtggta actggtatct gaccagcggc agtgattctc
     3001 ctgaactgca gccggagcca gacccgatgc cgaatccaga gccaaacccg aatccagagc
     3061 cgaaccctaa cccgacacct acgccgggtc cggatctgaa tgtggataat gacctgcgac
     3121 cggaggcggg tagctacatt gcgaaccttg cagcagcgaa taccatgttc accacgcgtc
     3181 tgcatgagcg tctgggtaat acgtactata ccgacatggt gacgggtgag cagaaacaaa
     3241 ccactatgtg gatgcgccat gaaggtggtc ataataaatg gcgtgatggc agcggccagc
     3301 tgaaaaccca aagcaatcgc tatgttctgc aactgggagg cgatgtcgcg cagtggagcc
     3361 aaaacggcag cgaccgctgg catgttgggg tcatggcggg atatggcaac agcgacagca
     3421 aaaccatttc ctcgcgaacc ggttatcgtg caaaagcgag tgtgaacgga tatagcacag
     3481 gcctctatgc cacctggtat gccgatgacg agtcgcgtaa tggcgcgtat ctcgacagtt
     3541 gggcgcagta cagctggttt gataacacag tgaaagggga tgacttacaa agtgaatcct
     3601 ataaatcaaa aggatttacc gcttcactgg aagctggata caaacacaaa ttagctgaat
     3661 ttaatggcag ccagggaacg cgtaatgaat ggtatgttca gccgcaagca caggttacct
     3721 ggatgggagt caaagccgat aagcaccgcg aaagcaacgg aaccctcgtt catagcaacg
     3781 gtgatggcaa tgttcaaacc cgacttggcg taaaaacctg gctgaagagc caccataaaa
     3841 tggatgacgg taaatcccgc gagttccagc cgtttgtaga agtgaactgg ctacataaca
     3901 gtaaggattt cagcaccagt atggatggcg tgtctgtcac tcaggatgga gcccgaaata
     3961 ttgctgagat aaaaaccggg gtggaaggac agctaaatgc caacctgaat gtctggggga
     4021 atgtgggcgt tcaggttgcc gataggggat ataatgacac ctctgcaatg gttggcatta
     4081 agtggcaatt ctgatctgaa taatgagtaa aaggagagag agccgaacgg gagataattc
     4141 ggctctctgt gtcattttct tgcagccagc tttatccgtc agagtgaaga ctcgcagatt
     4201 tagctggcgc acaagattta atcaaggtgt gttcaatggc ttcgcatgat ttgagtgttt
     4261 ttttggaaga gttcggggca acggtgaatt tgacgttgcc tggtatcgtg tccgaaaaag
     4321 aacggctttt actcaagctg ctaatggagg gaatgtcggt tacagaaatc tcacagtaca
     4381 gaaatcgcag tgcaaagaca atttcacatc aaaagaagca gctgtatgaa aaactaggca
     4441 tccagagtga tattaccttc tggcgggata tcttttttca atatcatccg caagtcatta
     4501 gtggcacggg aaataaaaat aatttttata tccctgataa ccgttgtcac catatcgtca
     4561 cgcctgaagc catcagtctg gcgttggaaa accatgaatt taaaccgtgg atccaaccgg
     4621 ttttctgcgc gcagactggg gtactgacgg gctgtgaagt gcttgtccgc tgggaacatc
     4681 cacaaacggg aatcatcccg ccggatcagt ttattcctct ggcggagtca tccggtctta
     4741 ttgtcataat gacccgccaa ctgatgaaac agactgcgga tattctgatg ccggtaaaac
     4801 atttgctgcc ggataatttc catattggta tcaacgtctc ggcgggttgt tttttggccg
     4861 cgggatttga aaaagaatgc ctgaacctgg tcaagaaatt aggaaacgat aaaatcaaac
     4921 tggttctcga gctaacggaa cgtaacccta ttccggtaac gccagaagcc agagcgatat
     4981 ttgacagcct tcatcagcat aacattacct ttgcgctgga tgactttggt acgggttatg
     5041 cgacctatcg ttatctgcag gcgtttccgg tcgattttat taagatcgat aagtcatttg
     5101 tgcaaatggc gggtgtcgac gaaatatccg gtcatattgt ggacaatatt gtcgaactgg
     5161 cgcgtaagcc tggtctgagt atcgtggcgg aaggggtaga aacccaggag caggcggatt
     5221 taatgatcgg taaaggcgtt cactttttgc agggctattt gtactctccg ccagtaccgg
     5281 gtaataaatt tatctctgaa tgggtaatga aagcaggtgg ttgatgtaaa ccgctattca
     5341 cagcgcatcg ggaggttggc agcgattaat tctcccgatg cagtaaatcc tgatagatcc
     5401 ccgttaatat gccatttggc gcaaattgtt ttttaatcca ctgcgtcacc tgacccgtag
     5461 cagaatgctg agtcgccagt aacatgcgtg aatcctggcg cgggttatgg atttgtcggg
     5521 taaccagcag ggattgcgcc atcgcctcac ggaccatgta atctggcaaa aaaacaatcc
     5581 cttcacctaa aatctggcac tgacatttgg tgttgaaatc tggcaccaga attgactcct
     5641 gcccgtgcag caaccagccc acttttttat taatcgtatg cgcggtgtcc tccaccataa
     5701 tattagggta gagacgcagt tggctttcgg cgatggttcc ggcacaaagg ctaacgggtg
     5761 atccggggcg atagcaaaag cccagcgaat cgcgccaatt tcagtgtaat caataccgcc
     5821 gccgtccagc agcgtgtcgg gtgcgccgat agcgatattt gcctgattat taataatcgc
     5881 atcccagacg ccgttataca cttcggtggt gacggtaatc tggcaggtag gaaactgctt
     5941 tttcagcacc tgtaataagc gtgcggtgtg tttgggcgta taaagcaact gattaataca
     6001 aatgcgcaca cgcgcttcta tgccctgcga aatagtatcg atgctgcgtt tgatggcgtg
     6061 aaaatcattc agcaggtcgg tagctttgcg gaaaaaataa cgcccggact ccgttaactc
     6121 aatgctgcgg gtactgcgag tgaacagcac cacatccagc cctgtttcca ttcgcttgat
     6181 ggtgtagcta atggcggaag tggttaaacc caactcttct gcggctttac tgaaactgcc
     6241 aaaacgcgca gcggtagtaa atgccaacag attctcttcg gtaaaaattg agttcattaa
     6301 accattcccg ccattatcta atatgaattt ctccaagcat aactgtagag gtatcaatta
     6361 gagatgggta atcatttttg aataactttt aatacccgtc gcgtttagtc gtcgcttcgc
     6421 ttaatttttg tccgccatat tctgaaatta tccccacgca gttacccgcc agaaaaccca
     6481 gtaacgtcac cgtccagttc atcccgctgg caaaaaacag cgtcatgccg agaaagccac
     6541 cgggaataaa tgacagtaac cagaaacgcc cttgccagac caccaccgcc gagaagggta
     6601 cggtagtaat tacgctcgcc cagaacgcgg acagtccact tacagaggcc agccaaccgg
     6661 cagccagcgc ggcgaaaaac gcccacacca tcccggaata attaaccgac agacttctga
     6721 taaaccccga tttaccgccg cctgcggcat aaaaactgca aaaggcgacg aagccaatgg
     6781 ttcctaataa ttcctaataa ttcccatccc tgagagagcc cgacatgtga agacactaac
     6841 tgccaaagcc ctgcacaaat accgacggtg acacccgttg ccgtgagtcc gttcatcttg
     6901 ttcccctctg gttagacatg gacgaaagct actcttacat gtgcagacta tagagaaata
     6961 aaacctgatt gagtgaaagg gtatcgggtc aaagaaacaa atattgcact accgcacact
     7021 gcgaaaagat tgttgaacat cattcaacaa aaaggcgaaa tacggcgaat taaatttaag
     7081 cgggttcttt tatcagatta tcgttgctat tctcaattca tcgaaaacag ccacaaaaac
     7141 caggggaatg tgaatgagta taaaaaatct acctgccgac tatttattgg ctgcacaaca
     7201 gggtgatatc gataaagtaa aaacctgcct tgcgctgggt gtcgatatta atacctgcga
     7261 tcgtcagggg aaaacggcaa ttacgctggc aagtttatat cagcaatatg cttgcgttca
     7321 ggcattaatt gatgccggag cggatattaa taagcaagat catacctgtt taaatccttt
     7381 tttaattagc tgtctgaacg gtgatttaac gctgctacga attattttac cggctaaacc
     7441 agaccttaat tgcgtcaccc gttttggcgg tgtcggcctg acgcctgcct gtgaaaaagg
     7501 ccatttaagt attgtaaaag agcttttggc gcatacggaa attaacgtta accagaccaa
     7561 ccatgttggc tggacgccac tactggaagc gattgtgctt aacgatggcg ggattaaaca
     7621 gcaggcgatt gtgcagttat tgctggaaca cggtgccagc ccgcatctga ccgataaata
     7681 tggcaaaacg ccactggaac tggcgcggga acggggcttt gaagagattg cgcagttact
     7741 gattgccgca ggtgcataaa ccgggaggct tgctatcaac acaccagaaa aacggtgtgt
     7801 gtgggcgcta actgcggatg cggattttct ggcgcagcga gggcaaggac aggttgaaca
     7861 ggtctttgcc agagcggtaa atatcgcact cccggctcgc cagcagttgc tgacgctgct
     7921 ttgtgaagag tacgacaatg cgccaaacag ttgtcggttg gcactcactc actttgatga
     7981 tctgttccgg catggtgata aggttcagtt tgacgatcaa ggtattacgg ttggtcaaca
     8041 tcttcatata gagatgagtc gttgtcggcg ttggctgtcc ccaaccttgc aaatgaccgc
     8101 tgtgaatttt cgccttatcg cctggcaaca gtggcacgac atcattcatc agcacctggg
     8161 ggaaaatgaa accctgttta attatcgcgg cgataatccg ttttatcagg cgttaaataa
     8221 agaattacat attaaacgac gggcagttat acaggcagta atcgataaac aaaatatcgc
     8281 ctcagcggtc gccagtatga tgggcttagg gattggcctt acgccatcag ccgatgatta
     8341 tttaacaggt ctggcactta ttttatttat ttccgggcat ccggcgggaa aatacaaaga
     8401 ggaattttat ctcggcttgc aacgcggcag aaataatacc acgttattaa gtgccataac
     8461 gctggaagcc gcattacaac aacgctgccg ggaaaatatt catcgtttta ttcacaacat
     8521 tatttatgac atccctggga acgcaactca ggcaatagaa aaaattaaac atattggctc
     8581 cagttccggc tgcgacatgc tgtatggcat ggccgatggt tgtgcgctga gccaaaccta
     8641 cggagggaat tatgtcagtt aaaatagtca ttaaaccgaa tacctatttt gattctgtct
     8701 cgctgatgtc tatctccacg cgtgcaaata aactcgacgg cgtcgagcag gcatttgtgg
     8761 cgatggcgac cgaaatgaac aaaggtgtgc tgaaaaattt aggactgctg acgccggagc
     8821 tggagcaggc gaaaaacggc gacctgatga ttgtcatcaa tggtaaatcg ggggcggaca
     8881 acgagcagtt gctggtggag attgaagaac tgttcaacac caaagcgcaa agcggctcgc
     8941 acgaggcgcg ttacgccact attgccagcg ccaaaaagca tatcccggaa agtaacctgg
     9001 cggtgatttc ggtcaacggt ctgtttgccg ctcgcgaagc gcgtcaggcg ctgcaaaacg
     9061 atctcaacgt gatgctgttt tccgataacg tctcagttga agatgaactg gcgctcaagc
     9121 aactggccca cgaaaaaggg ctgctgatga tggggccaga ctgcggcacg gcgattatca
     9181 acggtgcggc gctctgcttt ggtaacgccg tgcgtcgcgg caacatcggt attgttggcg
     9241 catccggcac cggcagtcag gagttgagcg tccgcattca tgaatttggc ggcggcgttt
     9301 cgcaactgat tggcaccggc gggcgcgacc tgagcgagaa aatcggcggc ctgatgatgc
     9361 tcgacgccat cgggatgctg gaaaacgatc cgcaaactga aatcattgcg cttatctcca
     9421 aaccgcctgc gcctgcggtg gcccgcaaag tgctggaacg tgcgcgcgcc tgccgcaagc
     9481 cggtggtcgt ttgcttcctc ggtcgtggcg aaacgccagt ggatgagcag gggctacagt
     9541 ttgcccgcgg caccaaagag gcagcgctaa aagcggtgat gctctccggc gtgaaacaag
     9601 aacatctcga cctgcatacc ctgaaccagc cgttgattac tgacgtgcgc gctcgcctga
     9661 agccgcagca gaaatacatt cgtggcctgt tctgcggtgg cacgctgtgc gatgaaacga
     9721 tgttcgcggt gatggaaaaa cacagcgatg tctacagcaa catccagccc gatccggaat
     9781 tccgcctgaa ggatatcaac cgcagcatca aacacacctt cctcgacttt ggcgatgacg
     9841 acttcaccaa cggcaagccg cacccgatga ttgaccctac caaccgcatc agccgcttga
     9901 tcgaagaggc gcgcgatcca gaagtggcgg tgatcgtgat ggattttgtg ctcggatttg
     9961 gatcgcatga agatccggtc ggttccacca tcgaggcgat caaagaagcg aaagcgatcg
    10021 ccgctgccga aggacgcgag ttgatcattc tcgcctatgt gctgggtacc gatcttgata
    10081 cgccatcgtt agaacaacaa agccagatgc tgcttgatgc cggcgtgatt ctggcgagca
    10141 gcagcaccaa taccggattg ctggcgcgtg aatttatctg caaaggggag gaagcctgat
    10201 gagccagtca ctgtttagcc aaccattgaa cgttattaac gtcggcatcg ccatgtttag
    10261 cgatgacctg aaaaagcagc atgtagaagt gactcaattc gactggacgc cgccggggca
    10321 gggcaatatg caggtggtgc aggcgctgga taacattgcc gattcgccgc tggcagacaa
    10381 aatcgccgcc gctaaccagc aggcgctgga gcgcattatc cagtcgcatc cggtgctgat
    10441 tggttttgat caggcgatta acgtggtgcc gggcatgacg ccgaaaacca ttcttcacgc
    10501 cgggccgccg atcacctggg aaaaaatgtg cggcgcgatg aaaggcgcgg tcaccggagc
    10561 gctggtgttc gaaggactgg cgaaagatct cgacgaggcg gctgaactgg cggcttccgg
    10621 ggagatcacc ttctcgccgt gtcacgagca cgactgcgtg ggatcgatgg cgggtgtgac
    10681 ctcggcctcg atgtttatgc acatcgtaaa aaacaaaacc tacggcaaca tcgcttatac
    10741 caacatgagc gagcagatgg cgaagatttt gcgtatgggc gctaacgacc agagcgtgat
    10801 tgaccgcctg aactggatgc gtgatgtgca gggaccaata ctgcgcgacg cgatgaaaat
    10861 tatcggcgaa atcgatctgc gcttaatgct ggcgcaggcg ctgcatatgg gcgatgagtg
    10921 ccataaccgc aataacgccg ggacgacgct gctgattcag gcgctgacgc cggggattat
    10981 ccaggcgggc tattccgtcg cgcaacagcg cgaagtgttt gagtttgttg ccagtagcga
    11041 ttacttctcc ggcccgacgt ggatggcgat gtgtaaagcg gcgatggatg cggcgcacgg
    11101 catcgaatac agcaccgtcg ttaccaccat ggcgcgtaac ggcgtcgagt tcggcctgcg
    11161 ggtgagcggc ctgccggggc agtggtttac cggcccagcg cagcaggtca tcggcccgat
    11221 gtttgctggt tataagccgg aagattcggg gctggatatc ggcgacagcg ccatcacaga
    11281 aacctacggt attggcgggt ttgccatggc gaccgcgcct gcaatcgtcg cgctggtggg
    11341 gggcacggtg gaagaggcta tcgatttctc ccgtcagatg cgcgaaatca cccttggcga
    11401 aaaccccaac gtcaccattc cgctgctcgg ttttatgggc gtgccgtcgg cagtcgacat
    11461 cacccgcgtg ggtagcagcg gcattctgcc ggtgatcaac accgccatcg cccataaaga
    11521 tgcgggcgtc ggcatgattg gcgcgggcat tgtgcatcca ccattcgcct gcttcgagaa
    11581 agccattctt ggctggtgcg aacgttacgg cgtttgacct acctgttcat accggatgcg
    11641 gcgtgaacgc cttatccggc ctacgaatgg cgcaagaatc tgtaggcctg ataagcgtag
    11701 cgcatcaggc atttgtcacc attgccggat gcggcgtgaa cgccttatcc ggcctacaag
    11761 tggcgcaaga atttgtaggc ctgataagcg tagcgcatca ggcatttatc accattgccg
    11821 gatgcggcgt gaacgcctta tccggcctac gaatggcgca agaatctgta ggcctgataa
    11881 gcgtagcgca tcaggcattt gtcaccattg ccggatgcgg cgtgaacgcc ttatccggcc
    11941 tacgaatggc acgagaattg taggcctgat aagcgtagcg catcaggcat ttgtcaccat
    12001 tgccggatgc ggcgtgaacg ccttatccgg cctacgaatg gcacgagaat ttgtaggcct
    12061 ggtaagcgta gcgcatcagg cagtctggcg ttggtcataa ccccatcacc ctctgtagcg
    12121 gacataacaa catgaaagaa cttgtggtcg ttgccattgg tggcaacagc attatcaaag
    12181 ataacgccag ccagtcgatt gagcatcagg cggaggcggt gaaagccgtg gccgatacgg
    12241 tgctggaaat gctggcttcc gattacgaca ttgtgctgac ccacggtaac gggccgcagg
    12301 tcgggctgga tttacgccgt gcggagattg cccacgagcg cgaagggctg cccttaacgc
    12361 cgctggcgaa ctgtgtggca gatacgcagg gcggcattgg ctacctgatc caacaggcgc
    12421 tgaacaaccg gctggcgcgt cacggcgaga aaaaagccgt caccgtggtg actcaggtgg
    12481 aagtggataa aaacgatcca ggttttgccc atcccaccaa gcccattggt gcattcttca
    12541 gtgaaagcca gcgtgacgaa ttacaaaagg caaaccctga ctggcgtttt gttgaagatg
    12601 ctggacgggg ctatcgccgc gtggtcgcct cgccggaacc gaaacgtatt gtcgaagcac
    12661 ccgccattaa ggcgctgatc caacaaggct ttgtggtgat tggcgcgggc ggcggcggaa
    12721 ttcctgtggt gcgtactgac gcgggagatt accaaagcgt ggacgcggtt atcgacaaag
    12781 atctctctac cgcgctgctg gcccgtgaaa ttcacgccga cattctggtg atcaccaccg
    12841 gcgtcgaaaa agtctgtatc cactttggta aacccgagaa gcaggcgctg gatcgggtgg
    12901 atattgccac catgactcgc tatatgcagg aaggacattt cccgcccggc agcatgttgc
    12961 caaaaatcat cgccagcctg acgttcctgg aacagggcgg taaagaagtg attatcacca
    13021 cgccggaatg cctgccagcg gcgctgcgcg gtgaaacggg cacccacatt attaaaacgt
    13081 aaggacgtaa gatgaaagaa aataatagcc gccgtgaatt tctgagccag agcggtaaga
    13141 tggtcaccgc cgccgcgctg tttggtacct ctgtgccgct cgcccatgcg gcggtctctg
    13201 gcaccacaaa ctgcgaagcg aacaacacca tgaaaatcac tgacccgcat tactatctcg
    13261 acaacgtgct gctggaaacc ggttttgact acgaaaatgg cgtggcggtg cagacccgca
    13321 cggcgcgcca gaccgtggag attcagaacg gcaaaattgt cgcgctgcgc gagaataagc
    13381 agcacccgga tgccacgctg ccgcactatg acgctggcga taagctgatg ctgcccacca
    13441 cccgcgacat gcatattcat ctcgacaaaa cgttttacgg cgggccgtgg cgctcgctca
    13501 atcgcccggc aggcaccacc atccaggaca tgatcaaact cgagcagaaa atgctgccgg
    13561 agctgcaacc gtacactcag gagcgggcag aaaaactgat tgatttattg cagtcgaaag
    13621 gcaccaccat tgcccgcagc cactgcaata tcgaaccggt ttccggcctg aaaaatctgc
    13681 aaaatttgca ggcggtgctg gcgcgacgtc aggcgggctt tgagtgtgaa atcgtcgcct
    13741 tcccgcagca cggtttgctg ctgtcgaaat cggaaccctt aatgcgtgaa gcaatgcagg
    13801 cgggtgcgca ttacgtcggc gggctggacc cgaccagtgt cgatggcgcg atggaaaaat
    13861 ccctcgacac catgttccag attgcgctgg actacgacaa aggcgtcgat attcacctgc
    13921 acgaaaccac tccgtcgggc gtggcagcca tcaattatat ggttgaaacg gtagagaaaa
    13981 cgcctcaact gaaaggtaag ctgaccatca gccacgcctt tgcgctggct acgctcaacg
    14041 agcaacaggt agatgaactg gcgcatcgca tggcggcgca gcaaatttct atcgcctcga
    14101 cggtgccgat tggcacgctg catatgccgc tcaaacagtt gcacgacaaa ggcgtaaaag
    14161 tgatgactgg cactgacagc gttatcgacc actggtcgcc ttatggtctg ggcgacatgc
    14221 tggaaaaagc caatctctac gcgcagctct atattcgtcc taacgaacag aacctctccc
    14281 gttcgctgtt tctagccact ggcgatgtat tgccgctgaa tgaaaaaggc gagcgtgtat
    14341 ggccaaaagc gcaggatgac gccagctttg tgctggtgga cgcctcctgt tccgccgagg
    14401 cggtggcgcg tatctcgccg agaaccgcaa cgttccataa agggcaactg gtgtggggga
    14461 gtgtggcagg ttgatgcgca tttgaaacgc ctgcaacggt gaatagtaag agatttaagc
    14521 cccagggagt ggggcgggaa tggtttgcta atgaatgatc ctgaacttta taatctcccg
    14581 gcataatttc caggggatta tttgagagaa atacacaaaa gtgagtttta acaaaggtat
    14641 ttatcaggcc ataaatgtga cctgcttcgg ataaaaaaga ttcagtatcg aaaaaccaat
    14701 gatactcttg ctgatggtta ccaacagtaa ttaagatgtg atttgttttt ccgtaatcgc
    14761 aaaaatattg ccatgaccat aaaagaaaaa ttctcacaaa aatatcccca tgcttccttt
    14821 tgtacttttg gcgattcagc cgcgttggcc gatcacctcg caacgttgat tgcgactggt
    14881 gttaaaacag cttcctgtgg ttcgctggcg ggatgtattg aagacaacgc atttccgatg
    14941 attggtgagt ataagattgt agaaaatagc cgtggtgagc ctgtatgtgt tatcagagtg
    15001 attggcctgc atttactgcg cttttctgat gtcaccgcgg agcttgcccg taaggaaggt
    15061 gaaggtgacc tcagtcttga atattggcgt aatgaacacc gtcgtttttt ccaggcagaa
    15121 ggaagctatt caccagaaat ggacgttatc tttgaagaat atgcccttat tgacgttgtg
    15181 tagataacta tttatctaca tgaaataagc tcacaaacat aaaatatcgc taattttgtt
    15241 tattttgctg ttttttacgt gatgaatatc ac
//
LOCUS       DBFIFM010000113        15105 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.113, whole genome shotgun sequence.
ACCESSION   DBFIFM010000113 DBFIFM010000000
VERSION     DBFIFM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.113"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(503..1594)
                     /gene="ychF"
                     /locus_tag="N7C74_002964"
     CDS             complement(503..1594)
                     /gene="ychF"
                     /locus_tag="N7C74_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HGU2432794.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1711..2295)
                     /gene="pth"
                     /locus_tag="N7C74_002965"
     CDS             complement(1711..2295)
                     /gene="pth"
                     /locus_tag="N7C74_002965"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HGU2432795.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2573..2851
                     /gene="ychH"
                     /locus_tag="N7C74_002966"
     CDS             2573..2851
                     /gene="ychH"
                     /locus_tag="N7C74_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HGU2432796.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2906..4585)
                     /gene="dauA"
                     /locus_tag="N7C74_002967"
     CDS             complement(2906..4585)
                     /gene="dauA"
                     /locus_tag="N7C74_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HGU2432797.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4710..5657)
                     /gene="prs"
                     /locus_tag="N7C74_002968"
     CDS             complement(4710..5657)
                     /gene="prs"
                     /locus_tag="N7C74_002968"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HGU2432798.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5808..6659)
                     /gene="ispE"
                     /locus_tag="N7C74_002969"
     CDS             complement(5808..6659)
                     /gene="ispE"
                     /locus_tag="N7C74_002969"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HGU2432799.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGANLS
                     PLHRAML"
     gene            complement(6659..7282)
                     /gene="lolB"
                     /locus_tag="N7C74_002970"
     CDS             complement(6659..7282)
                     /gene="lolB"
                     /locus_tag="N7C74_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HGU2432800.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7496..8752
                     /gene="hemA"
                     /locus_tag="N7C74_002971"
     CDS             7496..8752
                     /gene="hemA"
                     /locus_tag="N7C74_002971"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HGU2432801.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8794..9876
                     /gene="prfA"
                     /locus_tag="N7C74_002972"
     CDS             8794..9876
                     /gene="prfA"
                     /locus_tag="N7C74_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HGU2432802.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9876..10709
                     /gene="prmC"
                     /locus_tag="N7C74_002973"
     CDS             9876..10709
                     /gene="prmC"
                     /locus_tag="N7C74_002973"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGU2432803.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALARERPDCEITAVDRMPDAVALAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10706..11098
                     /gene="sirB2"
                     /locus_tag="N7C74_002974"
     CDS             10706..11098
                     /gene="sirB2"
                     /locus_tag="N7C74_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HGU2432804.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLTLVVLYIIIKLATTKVPLLG"
     gene            11102..11911
                     /gene="sirB1"
                     /locus_tag="N7C74_002975"
     CDS             11102..11911
                     /gene="sirB1"
                     /locus_tag="N7C74_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HGU2432805.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11947..12801
                     /gene="kdsA"
                     /locus_tag="N7C74_002976"
     CDS             11947..12801
                     /gene="kdsA"
                     /locus_tag="N7C74_002976"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HGU2432806.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLRQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12949..13056)
                     /gene="ldrA"
                     /locus_tag="N7C74_002977"
     CDS             complement(12949..13056)
                     /gene="ldrA"
                     /locus_tag="N7C74_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU2432807.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13485..13592)
                     /locus_tag="N7C74_002978"
     CDS             complement(13485..13592)
                     /locus_tag="N7C74_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU2432808.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13991..15091)
                     /gene="chaA"
                     /locus_tag="N7C74_002979"
     CDS             complement(13991..15091)
                     /gene="chaA"
                     /locus_tag="N7C74_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HGU2432809.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT         3798 a         3814 c         3878 g         3615 t
ORIGIN      
        1 ttgatgtatc tgcataacaa aaacaatatg cgccattttt gcattactaa taagaaaaag
       61 caaaaaacat cgatattcat ttttggtcaa tgggttttct ggtctatcgt tgttattgtg
      121 ctactggtta tcattttcat cttgccagga tcgcacgtaa tcacgttatt cggggacttt
      181 atctgcggga tattaccctt caggtaagtc cttttctcgc cagcatattg gagcgtggtc
      241 aatattgatc ggctgtgaag ggggctgatg tgtagtggaa caaaaactga cgttatatca
      301 ctgctaatga gatatccgtc atctgaacac tgatgtcttt aattattctc gtggttcact
      361 gtaggcaata agcacaaaag tgtaggatgt tacaagaatg attaggactc ggtgaaatga
      421 aaaatccacg caattgcgtg gattttatat acttttgcgc tcttcatgag atttagcgaa
      481 acctcatgag acaataaatt aattagacgt tgaaaaggaa gttcatcaca tcgccatctt
      541 tcacgatgta atctttacct tctgcacgca ttttgcctgc ttctttcgcg ccttgttcac
      601 ctttgtaagt gatgaaatct tcaaacgaga tggtttgtgc acggataaag cctttttcaa
      661 aatcagtatg gattttgccc gctgcctgcg gcgcggttgc tccaaccgga atggtccatg
      721 cacgcacttc tttcacccca gcggtgaagt aagtttgcag gttcagcagc ttataaccgg
      781 cacggatcac acggttcaga cccggctctt ccagcccaag ctcctgcata aactcgtcac
      841 gttcttcgtc gtccagttcg gcaatgtctg cttcaacagc agcacaaacc ggaaccacaa
      901 cagaaccttc tttcgccgcg atttcacgca cctggtcaag atatgggttg ttttcaaaac
      961 cgtcttcgtt gacgttggcg atgtacattg ttggtttcag cgtcaggaag ctcaggtaac
     1021 gaatagccgc tttctcttca gcgcttaaat ccagcgcgcg cagcatacct gcgttttcca
     1081 gctggggcag gcatttttcc aggaccgcca gctcagcttt cgcgtcttta tcgccacctt
     1141 tggctttctt ctgtacgcga tgaatcgcac gttcgcaggt gtcgaggtct gccagcgcca
     1201 gttcggtgtt gataacttca atatcgtcag ccgggttaac tttgccggaa acgtgaatga
     1261 tgttgtcatt ttcaaagcag cgaacaacgt gaccgatcgc ttcggtttca cggatgttgg
     1321 tcaggaactg gttacccaga ccttcgcctt tcgatgcgcc ttttaccaga ccggcgatat
     1381 cgacaaattc catggtcgtg ggaagcgtac gttgcggttt tacgatttca gccagttgat
     1441 ccaggcgagg atcaggcatt ggtacgacgc ctgtgttcgg ctcaatggtg cagaatggaa
     1501 agttggccgc ttcaataccg gctttggtca gcgcgttgaa cagggtagat ttcccgacgt
     1561 tgggcaaacc gacgataccg catttgaatc ccatgattta aatcacctta atatcttaat
     1621 aatcaacctg ttattgctaa cagattgcag aaatggaaat aactttgcct attatacacg
     1681 gcactcggca aaaatgccgc agacaacgac ttattgcgct ttaaaggcgt gcaatcggtt
     1741 cgttgctttg gtcaagccat ctgtaaacca catttcagta caacgcgccg cttcgtcgat
     1801 ggcttcatca attaacttct gttcactaac aggcggtttg cctaacacaa aaccaacaac
     1861 tttgttttta tcgcccggat gaccgattcc gatgcgtaaa cggtgaaagt tagggttatt
     1921 acccaattta ctgatgatgt ctttcagtcc attgtgacca ccatggccac cgcccaactt
     1981 aaatttggcg acgccaggag gcagatccag ttcgtcgtgg gccaccagaa tttcgtccgg
     2041 attaatgcgg aaaaaactgg ccatcgccgc aacggctttg ccgctgagat tcataaatgt
     2101 agtcgggact aacaggcgga catcttcgcc tccaagggtg actcgcgaag tataaccaaa
     2161 gaatttagcc tcttcgcgca gcggagcgcg caaacgctct gccagtaagt caacgaacca
     2221 ggcaccagca ttatgtcgcg ttgcggcgta ttcagcaccg gggttcgcca ggccgacaat
     2281 caatttaatc gtcacgtttt tttgtcctga gtgtgtacat aactggcgcg tagtttactg
     2341 gttgcggccc cgcttgacaa aaaactgcgt atcaaatgca gataacgtaa taattgcctg
     2401 agtggactat tagaaagtca aggtgttcag gcgtttattt gtaaagtttt gttgaaataa
     2461 gggttgtaat tgtgatcacg cccgcacata acccacaggg tgttgtctat actttacaca
     2521 taaggaagag gggtattccc tgttacaacc cagaaagttc cggaggtgac atatgaaacg
     2581 caaaaacgct tcgttactcg gtaacgtgct catggggttg ggtctggtgg taatggtggt
     2641 cggcgtgggg tattcaatcc tcaaccagtt accacagttt aatatgcccc agtatttcgc
     2701 acatggtgca gtgctaagta ttttcgtcgg tgccattctc tggctggcgg gtgcccgtgt
     2761 tggcgggcat gaacaggtgt gcgaccgtta ctggtgggtt cgccactatg acaaacgttg
     2821 ccgccgtagc gataatcgcc gtcatagcta acaaataatg cagtttgctg accagtcagt
     2881 ttgcgctgac tggtcaatct cgtacttata aatccgccat cgccgcgcga cgattcggga
     2941 agaacgcgag gcgtcccggg atcggttgaa tgcctgcgcg agccatagtg cgcagtggct
     3001 ggaattccac gttgcacacg cgcagttcac atccttcggg cagacgcttc acaaaacgct
     3061 ggaacgcatc aagaccacca gcatcaagta ccggaacggc atcccacttc agaatcacaa
     3121 tccgtttgcc ttcaagacgt gactccaggt ccgtgaataa gccttcagca gcagcaaaaa
     3181 acagcgggcc aataacgcgc agaaccagga catcgtctgg aacatctacg actaccggtg
     3241 ccaggcgagt catacgtgcg atacgacgca taaacagcag cgatgccagc acgatcccca
     3301 cgctgatggc aataaccata tcaaacagca cggtcagcga catgcacagc agcatgacaa
     3361 tgatgtcatc tttcggtgca tgacgcagca agtcgaccac tttatgcgct tcactcatgt
     3421 tccacgccac catcaacagc agggctgcca tagcggaaag cggcagccag gagagcagcg
     3481 gtgccagtac cagcagggca agaataacca gaatagagtg gatcaccgcc gagatagggg
     3541 aagttgcccc ggcacggacg ttagcggcag aacgcgcgat ggcagcggta gcggtaatac
     3601 caccaaagaa cggagcgatg atattcccca gcccctgtcc aaccagttcg ctattcgcct
     3661 tgtgcttcgt cccggtcata ccatccagca ccacggcgca gagcagagat tcgattgcgc
     3721 cgagcattgc cattgagaat gccgcaggca gcagtgtgcg aatagaatcc caggttagcg
     3781 tgaattctga attaggcaga tcccacggca gcaccagttg cggtagcagt tgcggaatac
     3841 cgttaccctg agaaccatcg gccaggacgt agtggaattg cgaaccgatg gtagcaacat
     3901 gtccgccgag caggttaaca atccccatca ccgcgcaacc agccagcaag gccggaaggt
     3961 gaccgggtaa acgaatgccc agacgcggcc agaaaacaag aatacctagc gtcacaatgc
     4021 caatggcagc atcacccaca ttaatggtcg gcagcgccat aaataatgcg ccgacttttt
     4081 gtagataatg ttccgggaca tgggccattt gcagaccgag aaaatcttta atctgcatgg
     4141 taccgatggt gatcccgata cccgaggtga aacctaaggt gacggaaacc ggaatatact
     4201 caatcaggcg accaaagcgt gccagaccca tcagaatcaa aaagatcccc gacagcaagg
     4261 tcgcaaccag cagtcctgcc agcccaaact gttgcgaaac gggatagaga attaccacaa
     4321 atgccgcagt cggaccggaa acgctaaagc gtgacccacc cgtcagagca atgacaatcc
     4381 ccgcaacagc tgcggtatat aaaccgtact ggggtgccac accactacca atagccaacg
     4441 ccatcgccag cgggatagca ataatcccga cggttatccc ggcaatcagg tcacgggtaa
     4501 accgtgcggc agtatatttt tctttccagc aagcgtcgat cagagcgcgg aaaggcatca
     4561 catgtgagga aaatattttg ttcacaataa tgtttcatcc gtgagcgcat catctgtcaa
     4621 ctaaatggca ggtgaaggag gcataggtca tacaaatgga tattacagac aaaaaaaccc
     4681 gccgcagcgg gtctttgagc cgggttcgat tagtgttcga acatggcaga gatcgattct
     4741 tcgttgctga tacgacgaat cgcttcggcc agcatacctg acagggtcag agtacgcacg
     4801 ttcggcagtg atttgatttc atcgctcagc ggaatggtat cgcagacaac gacttcatca
     4861 attacagagt tacgcaggtt gttcgccgcg ttgccagaga agatcgggtg agtcgcgtac
     4921 gcaaatacac gtttagcacc acgttctttc agagcttcag cagctttaca cagcgtaccg
     4981 ccagtgtcga tcatatcatc gaccagtacg cagtcacgac ctgcaacgtc accgatgata
     5041 tgcatcacct gggaaacgtt cgcacgcgga cgacgtttgt cgatgattgc catatcggta
     5101 tcgttcagca gcttagcgat agcgcgggca cgcacaacgc cgccgatgtc cggagaaacc
     5161 acaattgggt tatccagatt cagctgcagc atgtcttcca gcaggatcgg gctaccaaat
     5221 acgttatcaa ccggaacgtc gaagaaaccc tgaatctgtt cagcgtgcag atccactgtc
     5281 agcacacggt caacaccgac gctggagagg aagtctgcaa ccactttcgc agtgattggt
     5341 acacgagcgg aacggacgcg acggtcctgg cgcgcatagc caaagtaggg gataacagcg
     5401 gtgatacgac ctgcggaagc acgacgcagg gcatcaacca taacgactaa ttccatcagg
     5461 ttgtcgttag taggggcaca agtggactgg atgatgaaaa tatcaccacc gcgtacattt
     5521 tcattaattt gtacgctgac ttcgccatcg ctaaagcgac ctacagcggc gtcgccgagt
     5581 gaagtgtaca ggcggttggc aatacgttgt gctagttccg gggtggcgtt accagcaaaa
     5641 agcttcatat caggcacgag aagaacctca ggcatgcgtc cattggtgga aagaatctgc
     5701 cgaaaactgt gcgggccagg catgatcctt tccaggcggt gtattaaaga gcgcgatgca
     5761 acgtctggaa caaggtgacg ttgtcaccga aactcagctt gcccggctta aagcatggct
     5821 ctgtgcaatg gggaaagatt agcgcctttc gccacaaagc cattgagcca ttccggggct
     5881 tgctctagca cctggcgggc ttcagactct gtatcaaatt cagcaaagac acaggcccct
     5941 gtcccagtca ggcgcgacgg ggcgtattct aacagccagg aaagcaccgc atcaacctcg
     6001 cgaaaacgtt ttcttgcgat aacctcgcaa tcattgctga attcacattt tagcaacgtt
     6061 tctattgacc tttttggcgt attgcgcggg agttcaggat ctttaaaaat caccggagtc
     6121 ggaatactta caccagggtg cgccaccaga taccacttct ctggcggatc caccggggtt
     6181 agtatttcac caacgccttc ggcaaacgcg gcatgccccc gaacaaagac aggaacatct
     6241 gcgcccagcg tcagccccat ttccgccagc tcatccatgc ttagcccgca ttgccagaga
     6301 tgatttaatg ccaccaggac cgtcgcggca ttggatgaac caccgccgag accgccgccc
     6361 atcggcaaac gcttgtcaat gctgatattc gcaccgcttc ccgtcggaag acgcccgctg
     6421 tctgccgcag ttttcatcaa caaccgcgct gcgcgaacga tcaggttatc ttcatgttcc
     6481 acgccttcaa cgggcgttaa cagacggata tccccatcgt cacgaagctc aatgctgatg
     6541 gtgtcgccgt aatcaagaaa ctgaaacagc gtttgcagcg tgtggtaacc atccgcacgc
     6601 tgaccggtaa tgtataaaaa cagattaagt tttgccggag agggccactg tgtccgcatt
     6661 atttcactat ccagttatcc atttttaact tgatgcgttg accaccgtcg gtgagttcca
     6721 tattggctgg catcgcaggt tgcgttttgg tgtcataacc accataaaca accttccagt
     6781 ttttgccatt ctggctgtag gtaatttcgc tcaggcggta ctggtcgtcc agtttgtagt
     6841 cggttgcatc acccggtaaa cctaaaatcc actggcgcaa gctgttgagc ggaattggca
     6901 ttccagtcaa tttgccaatc atctcttcgg cgtcatcggc ggtataacgc tgacctttat
     6961 tgtcgactaa ctgcacgtta cccggttgag cattcagctc cagttccgtg ctgcccaatg
     7021 ggttagtgag cagcagacgg tagcgatcct ggccggtttg ctgccagaaa aagcgggcgt
     7081 acactttttg ttggtcagaa atataagcga acgcgccgcg agtctgatac tgattaagat
     7141 tgcgcacgtc ttgctgatgc tgacgccatt gtggcgaatc cgggcttttg ccaggacctt
     7201 tgggcgtggt aacggaacag gcagtgagca caagagcagc cagcggtagc aggcggataa
     7261 gacgaaaatc gggcaggggc atagtgatga caagtccttg agatacgttg cagttataac
     7321 ccttaatgct agcgtcacca ttagtcatcg tctatgttca agttgtctta attgtagaaa
     7381 tctaacgggt tcgggcaatt actccaaaag ggggcgctcc cttttattga tctcgcgcat
     7441 acggtatgat gcgccgagac taaccttatc aacgttggta ttatttcccg cagacatgac
     7501 ccttttagcg ctcggtatta accataaaac ggcacctgta tcgctgcgag aacgtgtatc
     7561 gttttcgccg gacaaacttg atcaggcgct ggacagtctg ctcgcacagc cgatggtgca
     7621 gggcggggtg gtgctgtcga cgtgtaaccg cactgagctt tatcttagcg tcgaagagca
     7681 ggacaacttg caagaggcgt taatccgctg gctttgcgat tatcacaatc ttaatgaaga
     7741 agatctgcgt aaaagcctct actggcatca ggataacgac gcggttagcc atttaatgcg
     7801 tgttgccagc ggcctggatt cactggttct tggggagccg cagatcctcg gtcaggttaa
     7861 aaaagcgttt gccgattcgc aaaaaggtca tatgaaggcc agcgaactgg aacgcatgtt
     7921 ccagaaatct ttctctgtag cgaaacgcgt acgcactgaa acagatatcg gtgccagcgc
     7981 tgtgtctgtc gcttttgcgg cttgtacgct ggcgcggcag atctttgaat cgctctctac
     8041 ggtcacagtg ttgctggtag gcgcgggcga aaccatcgag ctggtagcgc gtcatctgcg
     8101 cgaacataaa gtacagaaga tgattatcgc caaccgcact cgcgaacgtg cccaaattct
     8161 ggcagatgaa gtcggcgcgg aagtgattgc cctgagtgat atcgacgaac gtctgcgcga
     8221 agccgatatc atcatcagtt ccaccgccag cccgttaccg attatcggga aaggcatggt
     8281 ggagcgcgca ttaaaaagcc gtcgcaacca accaatgctg ttggtggata ttgccgttcc
     8341 gcgcgatgtt gagccggaag ttggcaaact ggcgaatgct tatctttata gcgttgatga
     8401 tctgcaaagc atcatttcgc acaacctggc gcagcgtaaa gccgcagcgg ttgaggcgga
     8461 aactattgtc gctcaggaaa ccagcgaatt tatggcgtgg ctgcgagcac aaagcgccag
     8521 cgaaaccatt cgcgagtatc gcagccaggc agagcaagtt cgcgatgagt taaccgccaa
     8581 agcgttagcg gcccttgagc agggcggcga cgcgcaagcc attatgcagg atctggcatg
     8641 gaaactgact aaccgcttga tccatgcgcc aacgaaatca cttcaacagg ccgcccgtga
     8701 cggggataac gaacgcctga atattctgcg cgacagcctc gggctggagt agcagtacat
     8761 cattttcttt ttttacaggg tgcatttacg cctatgaagc cttctatcgt tgccaaactg
     8821 gaagccctgc atgaacgcca tgaagaagtt caggcgttgc tgggtgacgc gcaaactatc
     8881 gccgaccagg aacgttttcg cgcattatca cgcgaatatg cgcagttaag tgatgtttcg
     8941 cgctgtttta ccgactggca acaggttcag gaagatatcg aaaccgcaca gatgatgctc
     9001 gatgatcctg aaatgcgtga gatggcgcag gatgaactgc gcgaagctaa agaaaaaagc
     9061 gagcaactgg aacagcaatt acaggttctg ttactgccaa aagatcctga tgacgaacgt
     9121 aacgccttcc tcgaagtccg agccggaacc ggcggcgacg aagcagcgct gttcgcaggc
     9181 gatctgttcc gtatgtacag ccgttacgcc gaagcccgcc gctggcgggt agaaatcatg
     9241 agcgccagcg agggtgaaca tggtggttat aaagagatca tcgccaaaat tagcggtgat
     9301 ggtgtgtatg gtcgtctgaa atttgaatct ggcggtcatc gcgtgcagcg tgttcctgct
     9361 acggaatcgc agggtcgtat tcatacttct gcttgtaccg ttgcggtaat gccagaactg
     9421 cctgacgcag aactgccgga catcaaccca gcagatttac gcattgatac tttccgctcg
     9481 tcaggggcgg gtggtcagca cgttaacacc accgattcgg caattcgtat tactcacttg
     9541 ccgaccggga ttgttgttga atgtcaggac gaacgttcac aacataaaaa caaagctaaa
     9601 gcactttctg tactcggtgc tcgcatccac gctgctgaaa tggcaaaacg gcaacaggcc
     9661 gaagcgtcta cccgtcgtaa cctgctgggg agtggcgatc gcagcgaccg taaccgtact
     9721 tacaacttcc cgcaggggcg cgttaccgat caccgcatca acctgacgct ctaccgcctg
     9781 gatgaagtga tggaaggtaa gctggatatg ctgattgaac cgattatcca ggaacatcag
     9841 gccgaccaac tggcggcgtt gtccgagcag gaataatgga atatcaacac tggttacgtg
     9901 aagcaataag ccaacttcag gcgagcgaaa gcccgcggcg tgatgctgaa atcctgctgg
     9961 agcatgttac cggcagaggg cgtactttta ttctcgcctt tggtgaaacg cagctgactg
    10021 acgaacaatg tcagcaactt gatgcgctac tgacacgtcg tcgcgatggt gaacccattg
    10081 ctcatttaac cggggtgcga gaattctggt cgttgccgtt atttgtttcg ccagcgacct
    10141 taattccgcg cccggatacg gagtgtctgg tggagcaggc actggcgcgg ttgcctgaac
    10201 aaccttgccg tattctcgat ctcgggacgg gtaccggggc gattgcgctt gcgctggctc
    10261 gcgagcgccc ggactgcgaa attaccgctg tagatcgtat gcccgatgct gtcgccctgg
    10321 cacaacgtaa tgcccagcat ctggcgatca aaaatatcca cattctgcaa agcgactggt
    10381 ttagcgcgct agccgggcag cagtttgcga tgattgtcag caatccgccg tatattgacg
    10441 agcaggaccc tcatcttcaa caaggcgatg tccgctttga gccgctcact gcgctggttg
    10501 cggcagacag tggaatggca gacatcgtgc atatcatcga acagtcgcgt aacgcgctgg
    10561 tatccggcgg ctttctgctt ctggaacatg gctggcagca gggcgaagcg gtgcgacagg
    10621 catttatcct cgcggggtat catgacgtcg aaacctgccg tgactatggt gataacgagc
    10681 gcgtaacgct cggccgctat tatcaatgac aagtttttct acactgctta gtgttcatct
    10741 tattagtatc gcgctttctg ttgggctatt aaccttacgt ttctggctac gttatcagaa
    10801 gcatccacag gcatttgctc gctggacgcg cattgtgccg ccggttgtcg atacggtgtt
    10861 attgttaagc ggcattgcgt tgatggctaa agcgcacatc ctgccatttt ccgggcaggc
    10921 acagtggctg actgaaaagc tgtttggagt tatcatttat atcgttttgg gttttattgc
    10981 actcgattat cgtcgtatgc acagtcagca ggcgcgcatt attgccttcc cgctgacgtt
    11041 ggtggtgctg tacatcatca ttaaactcgc caccacaaaa gtaccgttac tggggtaagt
    11101 catgagatcg ttagctgatt tcgaatttaa taaagcgcca ttgtgcgaag gcatgatcct
    11161 ggcttgcgaa gcaatccgcc gcgattttcc ctcgcaagat gtttacgacg aactggagcg
    11221 tctcgttagt ctggcgaagg aagaaatcag ccagcttctg cctttagaag agcagctgga
    11281 aaaactgatc gcgctgtttt acggcgactg gggatttaaa gcctcacgcg gcgtttatcg
    11341 tctttccgat gcattatggc tggaccaggt gttaaagaat cgacagggca gtgcggtatc
    11401 attaggtgcg gttttattat gggtcgcgaa tcgtctcgat ttgccgctgc tgccggtgat
    11461 tttccctacg cagctgatat tgcgcattga atgtccggat ggcgaaattt ggctgattaa
    11521 tccttttaac ggtgaatcgt taagcgaaca tatgctggac gtatggttaa agggaaatat
    11581 cagcccgtcg gcggaactgt tttatgaaga ccttgatgaa gctgataaca ttgaggtaat
    11641 ccgcaaattg ctggatacac tcaaagcctc gttgatggaa gaaaatcaga tggagctggc
    11701 gttacgcacc agcgaagctt tattacaatt caacccggaa gatccctatg aaattcgcga
    11761 tcgcgggttg atttatgcgc aactggattg cgaacacgtt gcgttgaacg atttaagtta
    11821 tttcgttgaa cagtgtccgg aagacccgat cagcgaaatg atccgtgcgc aaataaataa
    11881 catcgcgcat aaacatattg tgctgcatta attaatcgac attttactca aggttaaggc
    11941 gatcctatga aacaaaaagt ggttagcatt ggcgacatca acgtagcaaa tgacctgccg
    12001 ttcgtactgt ttggcggtat gaacgtgctg gaatctcgcg atctggcgat gcgcatttgc
    12061 gagcactacg taaccgtgac ccagaaactg ggtatccctt acgtgttcaa agcctctttt
    12121 gacaaagcca accgctcctc catccactct tatcgtggac cgggcctgga agaagggatg
    12181 aaaatcttcc aggagttgaa gcagactttt ggcgtgaaaa ttatcaccga cgttcacgaa
    12241 ccaagtcagg cacagcccgt tgctgatgtc gtggatgtga ttcagttgcc ggcgtttctt
    12301 gctcgccaga ctgacctggt tgaagccatg gcgaaaaccg gtgcggtaat taacgtcaag
    12361 aaaccacagt ttgtcagccc gggacagatg ggtaatatcg ttgataaatt caaagaaggc
    12421 ggcaacgaaa aagtgattct ttgcgatcgt ggtgctaact tcggctatga caacctggtt
    12481 gtcgatatgc tgggcttcag cattatgaag aaagtgtctg gtaactcgcc ggtgattttc
    12541 gacgtgaccc acgcattgca atgccgcgat ccttttggcg cagcttccgg cggtcgtcgt
    12601 gctcaggtgg ctgagctggc acgagccggt atggcggtag gtctggcggg gctgtttatt
    12661 gaagcgcatc cggatccgga acatgcgaaa tgtgatggtc catccgcgct gccgctggct
    12721 aaactggaac cgttcctcag gcagatgaaa gcgattgatg atctggtgaa aggtttcgaa
    12781 gaactggata ccagcaagta atcttttttg cttgaaaaat aaagtcttag cgttctgcgt
    12841 taagactttt ttcatgggtg ccggatacaa aaaaggccgc aggctgttac cctgcggccg
    12901 gtttcgggcg catattgcca tcacggcagc ctgacgcccg ttttcgcctt acttccggtt
    12961 acgccaccag ccgacaatcg ctgcggtaat aattcccgcc aggatcggtg ctgccaggtc
    13021 gtgccagaaa atcatggcaa actgcgcgag cgtcatatag ccgccttgtt gtaatgacaa
    13081 cattttgcgg ctattcttga attgttgatg gttcaagatt agcccccgtt ctgttgtcag
    13141 gttttacctc tcaacgtgcg ggggttttct ctttccagca accaatgcca ccagggataa
    13201 agcccccgca acattgcgcc tcaccggata acgccggctt ggtgtggata ctacgactca
    13261 attcatcttc acttcatccc tgaaatgttt gcaatgaaga gtgcattccg gtttttcaac
    13321 agctgttaca gtcgtttcat gagtgctctg gatgatgctt ccagctcggg ttgccaatat
    13381 ttacttgtgg aagtgataaa gacaaaaatg gccgcaggct gttacccctg cggccggttt
    13441 cgggcgcata ttgccatcac ggcagcctga cgcccgtttt caccttactt ccggttacgc
    13501 caccagccga caatcgctgc ggtaataatt cccgccagga tcggtgccgc caggtcgtgc
    13561 cagaaagtca tggcaaactg cgcgagcgtc atatagccgc cttgttgtaa tgacaacatt
    13621 ttgcggctat tcttgaagtg tctggtttca agattagccc tgttgtcagg ttttacctct
    13681 caacgtgcgg gggttttctc tttccagcaa ccaatgccac cagggataaa acccccgcaa
    13741 cattgcgcct caccggataa cgccggcttg gtgtggatac tacgtctcaa ttcatcttca
    13801 cttcatccct gaaatgtttg caataaagag tacattccgg cttttcaaca gctgttgcag
    13861 tggtttcgtg caggttgtgg tacaggctcg caattctgat taacgacctg tccaggtagt
    13921 atgaagaaat acaataagca ggaatttata ttatctcccc cggtttccgt catcggagga
    13981 gatataactg tcaggcaaat atcgtcatca aataggcggc aaacagtgcc agatgcgctg
    14041 cgccattgag cacgttagta cgtccggtgg agaaggagat atggcacagc actaaagagg
    14101 ccaccatcac caccatttct ggcgcaccaa gtgcaaactg caattcgtta cccgtcataa
    14161 aggcaattag cgtgacgaca ggtacggtaa gcgaaattgt tgctaacact gaaccaaaga
    14221 acagattcat cgcgcgctgg acctggttgt tcaacactgc ttttaatgca cctaaacctt
    14281 ccggcgacag aatcaacagt gccaccagga agccagtaaa ggcgacaggg gcattcatgc
    14341 tgtcgagcaa agtctccagc gggctggcgt tcattttggt gactgcaata acggcaatca
    14401 ggtggataat caaccagata gcatgccaca ggctgctatg ggcagacggt ttaccgtggt
    14461 gcgggtcgtc gtcatcactg tcatcttcgt gctcgtagac aaacaaactt tgatgcgttt
    14521 tggtctggat cagcaaaaat acaccataca ttgccgcaga aattaatgct accagtaacg
    14581 cctgaccggt tgaaaaattc gccgcaggca gagccattgg aaataccagt acgattatcg
    14641 ccagggggaa cagcgcaatt aaatactgct taataccaaa cagattcata tattgggtgg
    14701 caaacttacg accgcccaac aataatgaaa agccgaccag cccaccggta acaatcataa
    14761 tgatcgaata gagcgtatca cgcattagcg ttggcgcggc gtcgccggtt gccattaaag
    14821 ctgaaatcaa actaacttca agaataacca ctgaaaggct aagaataagc gaaccgtaag
    14881 gttctcccag gcgatgggct aatacgtccg catgacggac aacactaaag gcgctactta
    14941 aaataccaat aagcgcaaga agattgatgg caatgaccac tggtagtgtc tggctgcttc
    15001 cccacaggaa cagcactacc agcgccagaa ccgggaaaat aagcgaagtc tccttgtggc
    15061 gggtttttac cgcctcttga gcatttgaca ttatggttat ccctt
//
LOCUS       DBFIFM010000114        15075 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.114, whole genome shotgun sequence.
ACCESSION   DBFIFM010000114 DBFIFM010000000
VERSION     DBFIFM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.114"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            87..821
                     /locus_tag="N7C74_002980"
     CDS             87..821
                     /locus_tag="N7C74_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU2432810.1"
                     /translation="MKSIATLVVCAISGIACVNLSAHAAEGNHTISLGYAHFQFPGLK
                     DFVKDATAHNRETFSHFVNRNYFSSLGEYTDGRVSGYEGKDKNPQGINIRYRYEITDD
                     FGVITSFTWTRSLTNSQTFIDVQSADHTRKIKNPAASARTDIRANYWSLLAGPSWRVN
                     QYMSLYAMAGMGVAKVSADLKIKDNINSSGGFSESNSTKKNSLAWAAGAQFNLNESVT
                     LDVAYEGSGSGDWRTSGITAGIGLKF"
     gene            1052..1192
                     /locus_tag="N7C74_002981"
     CDS             1052..1192
                     /locus_tag="N7C74_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU2432811.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            1249..1650
                     /locus_tag="N7C74_002982"
     CDS             1249..1650
                     /locus_tag="N7C74_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432812.1"
                     /translation="MAPVSGERMNDKILRYMQRVVRNSRNPEFMNEVKDACLKKQAFC
                     FEAPDGFLVLRSVLSADGIPYVLVLLGVCTGSNSVERYLPEVKTLTRLAGGRWAEFHT
                     ARRGFIRLGKRLGFERMPDDEDGFMVFRIAV"
     gene            1745..2401
                     /locus_tag="N7C74_002983"
     CDS             1745..2401
                     /locus_tag="N7C74_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432813.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYKNILRPAEDNFM
                     EKVDDLNSEQQYDNIAGTTNLGYQKQFGEARKELAGNLAQSGVDPSSGRFNAVMNANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAVGAYAMHKAGGSGGSGGAKTPGTGANAIQYQAQNWRL
                     "
     gene            2404..2850
                     /locus_tag="N7C74_002984"
     CDS             2404..2850
                     /locus_tag="N7C74_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432814.1"
                     /translation="MEYGKYETLARYGYTGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVSRPDNPDSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQKLSVGGQLVAA"
     gene            2860..3153
                     /locus_tag="N7C74_002985"
     CDS             2860..3153
                     /locus_tag="N7C74_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HGU2432815.1"
                     /translation="MGYGLLDIANQSRREALQGISEADRRREEIEASNKMMAAQQKAQ
                     KKQNIATGIGTGAAVGASVAGSSAAMGATLGAWAGPVGIAAGAVIGGIAGALF"
     gene            3164..4429
                     /locus_tag="N7C74_002986"
     CDS             3164..4429
                     /locus_tag="N7C74_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432816.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQSQFEHRKENDQRTYDLNVRNADREYALKEREHKAAQNYRNASLGLSQQRLQLEKY
                     NQRRLEYNDMIAHSQPLMEALGKAIEAGDQEAATRLFGQLPKGHPLILMSNEGYAAKA
                     GQAVINLQKIFGDKPDMAIDSLNTPENLDVLSGVFAPELQQRIGMPDSTGEKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAEISKNYGYFMPKQQGLSLKELQKGASNVAADAIKNGGNAQAAVDEYYAATGSQ
                     PHQQKIQQQKLQQQVINWAGDDPDKLSFARNVAARQPEMLEPQNQKLLENGYANFLRI
                     QKARGEQARDESASSASQFIRGLKQNYAQ"
     gene            4498..12873
                     /locus_tag="N7C74_002987"
     CDS             4498..12873
                     /locus_tag="N7C74_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HGU2432817.1"
                     /translation="MAYSEEQRPEAQLGNQNRNSLNIQQPGETDSYEAFFSDPNRWKD
                     NSTSFSLGDVLPTMGKGFAQSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPG
                     VATVQDIFAGGSRGADEVIDTLPDGKNAVTDTVGKGLKATGKAVSDGAKATDEWLTGK
                     MSPGAVRALNTPMTEGYDDSAVWVAKGVNLIGALVPDMVAGGVAKKVGDVTLRKMLTA
                     GLEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAAADAVLN
                     ADYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAV
                     NAMAAKLGDAQLFNLVTRGTAKTVKSGIVRNATEQGAINAAQGGYSRYQENTALRETA
                     GMDVSPWEGVADATIEGAAFGAAMGAPSGAVAGYRGRRQAAEEAAMRDAETVQQDDAA
                     PQPESVDPVAQHRESMQGMNREQLLEQYADADMATEGDASAAHRREAASQLLNELDEQ
                     AKRQAVMDELKAKPRPELLEEYRRLSLKEGRTETEEQQLQAIRDVLRPQQEARPEAQP
                     ENTDDGDGSIYPTVRFRDPDEVRIEINGSGASRPAERIEKVRPDNRYFTDEKSAMGSD
                     VFRNASATGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERITDADPMEQP
                     AFMRDPRFRGFTGDDTEVQARLARGNVPTAEELVRSQMAEGDAGPTAQELTERPRLPA
                     PGDIHPGQGYPLPGEVARTPDENQAGRGGRFTITGEVKGQSFQKGRAPAPENAAGRQG
                     ETLEGETVRRGLPSPDAQNATAPVREGLPAPDIARNVRMPQPESLPRTVRDSLPELAQ
                     QAEVRRQARENRDILQPETIAPESETTVSTDREATVRGGEVRGKKIEDFGEEIKGAAK
                     HRYAQLAETLGKTLEDRDYVTQPLSKLFPKPDYAKLANEGADADTLAMIALYRSDIPA
                     KTKLNAVKWVESVKSIRTSVAGMLEGKVTAGRLAEWMEGRMPARYADTWQLLRTLPPS
                     QIDKASAYRVVSGVYQAAGGKRYDPPRKLYSLRNKDNKGSNLFFSESRDELLTKAKAW
                     FAEQEEKSQAKGDEKTALSPDDKISFDVYRNTRSGDIFIAYGKNKMRVRGGFKSASDA
                     RKYIDSHRDELVRHVKEMREISREEQRNATNRDRTGPERRKGDVSPKQFSDAFGFRGV
                     QFGNYVEGPRRQADLNRAYDSLHDLAEVLNVPTKALSLNGRLGLAFGARGKGKAAAHY
                     EPGEVAINLTKGNGPGALAHEWFHSLDNYFGRYDVSTDGKITSGGDFMTEARRVKRVF
                     KDGRFVDAEYPVRQEVYDAFKGVIEAIKNSDMPRRSALLDEVRSKPYWSTDVEMAARA
                     FERYVQDKARKAGVENDYLVNIRKAPEHNTDNTYAYPTNAELDGGIREAFDHLFRTLK
                     TRETDKGVAFYSRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVARGVMSGIKDSDL
                     KVRVVKSQKEAEALAGELFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHEII
                     HHAMEHVVTPAEYQTIIKTVLKTRDSDNVTIREAWRKVDASYGKESPEVQAGEFLAHM
                     AEKQPNKFVAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYTPR
                     EDGAGASSQYSRSGKRDPFKVPEGEGERYRDDLARMMKSLRTTDLTVNIGRTPPVLRH
                     LGAPDLPLVISRDTVRKATNGVKHVVPMDVIERLPELMHDPDAIYRSATERNAVVMLL
                     DAVDKNGDPVVSAVHMKAVRSRLEINKVASVYGTENGKKLKSMEMTGLTLYRREKLSR
                     DNLLHRGLQLPKGEHSYRGSADKILYPEDIRKGPYYSRTSSLTPEETIASRFVRQMQD
                     KFQVLKAVQENIRKTGGKVDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKLLADY
                     KIAQADLDEYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQAQY
                     DRLAGIIDDMLARRRELIREAGLEENGVVDAWQNAYRYYVPLKGQDVDGVVSLPRTGK
                     GFTIGGRESRQAMGRASRAQSPSTQAIQDLSESLIRHRKNEVGNAFLKLVQDNPDKDY
                     WQVFTDDRPDTMRTIAERKDQETGETIREVVERPVPMAMMADRYFTTKKNGKTYYIKL
                     HDPRLMRAMKNMGPETSNAFVRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVMNL
                     KAEQGRSDGKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGKGAQWQKVWKEFVE
                     DGGKTGWFNMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVENALR
                     LSAYKHARDAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTANLVRT
                     LGHLNGDGPLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYDKVP
                     SHVKERNLVIMKSVFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAAGNV
                     VGGVLGAFSPVGSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENMPFGT
                     PKPDSQLGRRSTPEAYKAFASWLNAFSGGSQYRSGAVDVTPESLKYWIDYISGGTGRF
                     ISKTTDAAVKSLNGIDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEVAQYHAELKSL
                     TGAERTAFIDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSTLSLAQQSAMVKSVE
                     RDMKIAVDRFNREYNKKVGVE"
     gene            complement(13095..14027)
                     /locus_tag="N7C74_002988"
     CDS             complement(13095..14027)
                     /locus_tag="N7C74_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HGU2432818.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            14321..>15075
                     /gene="mlaA"
                     /locus_tag="N7C74_002989"
     CDS             14321..>15075
                     /gene="mlaA"
                     /locus_tag="N7C74_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HGU2432819.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT         3839 a         3472 c         4415 g         3349 t
ORIGIN      
        1 agcgagtctt taatattcat ttaaaatgtc taaaaagatg tattattaaa aagtttagcg
       61 tgttatctga atacaggata tcttaaatga agagtatagc aacactggtt gtgtgtgcaa
      121 tctccgggat tgcctgtgta aatttatctg cacatgcagc agaaggaaac catacaattt
      181 ctctggggta tgcgcacttt cagtttccgg gactgaagga ttttgtaaag gatgcgactg
      241 ctcataacag ggagactttc agtcatttcg tcaacagaaa ctacttttct tcattgggcg
      301 aatatacaga tggtcgggtc agtggatatg aaggcaagga taaaaatcca cagggcatta
      361 atatcaggta tcgctacgag ataacggatg attttggcgt tatcacctct tttacatgga
      421 cgcgttctct cactaactca cagacattta ttgatgtgca gtcagccgat cataccagga
      481 agattaagaa tccggcagct tctgccagaa cggatatcag ggcgaattac tggagtctgt
      541 tagcggggcc ttcatggcgg gttaatcagt acatgagctt atatgcgatg gcagggatgg
      601 gcgttgctaa agttagcgct gacctgaaaa ttaaggacaa tattaacagt agtggcggat
      661 tttctgaaag caacagcacg aaaaaaaact cccttgcgtg ggctgcaggt gcacagttta
      721 acctgaatga gagtgttaca ctggatgtgg cttacgaagg ttccggctct ggcgactggc
      781 gcacgagtgg aattactgct ggcattggcc tgaaattcta acctgtatcc ggtaaccgtt
      841 tactacccgc tgtgatggcg ggttttttat tgcccgtaca gggcaaaaac cgtaaattat
      901 gcgtgggtgc ctttcggctg atggctggag ggtgaacctg aaggcctgat gtggaaaggc
      961 cccgagtcaa cttaacgtta acccgaggcc ctaacacttc gtaccttgac aagtagaagg
     1021 ttagcgcctc tccactcagg aggcaagcgc tatgtcgcaa aaatcgctta tcaccgtcac
     1081 aatttgtatg acggttatct tcaccatctg gatgttacac ggctcgttgt gtgagttccg
     1141 gctgaatttg tggggagcgg agtttgcggc gttcttacag tgtaagcagt aggaaaaccg
     1201 cggcggggac gaaagtcccc gtcaactggt tgctgaggtt cagccgatat ggcacccgtt
     1261 tcaggtgaga gaatgaacga taaaattctc cggtatatgc agcgtgtggt gagaaattcc
     1321 cgcaaccctg aatttatgaa tgaagttaaa gacgcctgcc ttaaaaagca ggcgttttgt
     1381 tttgaggcac ctgatggctt tctggtgctg cgttctgtgc tcagtgctga tggtatccct
     1441 tatgttctgg tgttgctggg cgtgtgtacg gggagtaaca gtgttgagcg ttatctgccg
     1501 gaggtgaaga cattaacccg tctggctggc ggacgttggg ctgaatttca tacggcaagg
     1561 cggggattta tccggctggg aaaacgtctg ggctttgagc gaatgccgga tgatgaggat
     1621 ggcttcatgg tgttcaggat agcggtctga ctgccacagt tttcatcatc gtgtttaaac
     1681 caacattgca attcacattc tgaccctgct ccggcagggt ttttttatta tccagggggc
     1741 cattatgggt ggaagtaaag gtggtggtga taccaaagta aaaccaacag cagcgcaaat
     1801 agcacaggaa gaagtggcct ggaaagggtg gcaggattac aaaaatatcc tccgcccggc
     1861 tgaagataac ttcatggaaa aggtcgatga cctgaacagt gagcagcagt acgacaatat
     1921 tgctggcacc acaaatctgg ggtatcagaa acagtttggc gaagcacgga aggagctggc
     1981 gggtaatctt gctcagtccg gtgttgaccc gtccagtggt cgctttaacg cggtaatgaa
     2041 tgcgaaccag agtgatcagg taaccgggca gattgacaca accacacggg ggcaggtatc
     2101 gcaggcagat aagtatgttg ccgggctaca ggatgttgca gctctcggtt caggtcagaa
     2161 ggcggatgcg ttacagagtt ttaactcgct ggcagacagc agtctggcaa aagctaaatc
     2221 ggacgcacag gctgcgttta cgaaacagca ggggcgagcc tctcttgttg gcgctggttt
     2281 gggtgcggta ggtgcatatg cgatgcataa ggctggtggt agcggaggaa gtggcggtgc
     2341 taaaacacct ggcaccggcg ctaatgccat tcagtatcag gctcagaact ggagactgtg
     2401 attatggagt acggtaaata cgaaacactt gcgagatacg gttataccgg agcagcccgc
     2461 cctcaggggg actggcagac atccgcagcg ctgacccgcc agcaatacga cgactggcgc
     2521 accagatatt taccccgtgt agcaaggctg gctgaccttg gcgagaacaa cagcctgatg
     2581 aatgcacagc ttgcccgggt gggcggcctt gccacttcca gtctccgtac agcgcagatg
     2641 gcgcaggata accagatggc gagatacggg gtaagccgcc cggataatcc cgacagtaat
     2701 acgctggggt tacgtaatgc cctggcaatt gctggcgcga aaaatggtat ccgtgaagcc
     2761 gaacaggatc gccagatgaa tattctgacg ggggcttctg caccggcaag acagaaactg
     2821 agtgttggcg gacaactggt ggcagcgtaa ggaggaaata tgggatacgg tttactggat
     2881 attgcaaatc agtcgcggcg tgaggcatta cagggaataa gcgaggcaga cagacgacgt
     2941 gaagaaattg aggcgtcaaa caagatgatg gcggcgcagc agaaagctca gaaaaaacag
     3001 aatatcgcca cgggcattgg tacgggggct gctgtgggtg catctgtggc aggctccagc
     3061 gctgcaatgg gggccacgct cggagcatgg gctggtcccg tcgggatagc tgctggtgct
     3121 gtgattggcg gcattgcagg cgctttgttt taaggagtgg tgaatgagcg gatttgcaca
     3181 ggggttactt gccggattca gcaccgttga ccaggcaatg acccgtcgta aggagcttgg
     3241 tttgcgagaa gcacagcttg ctcagcaaca gaaaaataac gagcgcgatt ttgaatttgc
     3301 gcagtcgcag tttgagcacc gaaaagagaa tgatcagcgt acatatgatc ttaatgtcag
     3361 gaatgctgac cgtgaatatg cgctgaaaga gagggaacac aaagcagcgc agaattatcg
     3421 aaatgcgtca cttgggctat ctcagcagag actgcaactg gaaaaataca accagcgacg
     3481 gcttgagtat aacgatatga ttgcccatag ccagccacta atggaagcgc ttggaaaagc
     3541 aattgaggct ggcgatcagg aggctgcaac gcgtctgttc gggcagctgc caaagggaca
     3601 tccattaatt cttatgtcaa acgaaggcta tgcagcgaaa gcgggtcagg ccgtgatcaa
     3661 cctgcaaaaa atctttggtg ataagccgga catggcgatc gattcgctga ataccccgga
     3721 aaatctcgat gtgctttccg gcgtgtttgc cccggaacta caacagcgta ttggcatgcc
     3781 tgattcaacc ggggaaaaaa cgataaaaga ggccaggatt ggcagtatcg taccggcgca
     3841 gcaggaaggg tacgtactta ttggccttga tctcacatac agcgatggct ccaccgcgca
     3901 taaacctgta acagaatacg gcagtgcgca ccctgatgat caaaccgtgc tggcgatacc
     3961 cgttgataag gctatcgctc aggtcaggga tcgcagcaaa tttgcagaga tatcgaaaaa
     4021 ttatggttat tttatgccga agcagcaggg actttctctg aaagagcttc agaagggggc
     4081 cagcaacgta gcggcggacg cgatcaagaa tggcggtaat gcacaggctg cggtggatga
     4141 atattatgct gcgactggtt cacaaccgca tcaacagaaa attcagcaac aaaaacttca
     4201 gcaacaggtt atcaactggg cgggagatga tcctgataag ctgtcatttg ccagaaatgt
     4261 agcggcccgt cagcctgaaa tgctggagcc tcagaatcag aaattgctgg agaacgggta
     4321 tgcgaatttt ctccgtattc aaaaggctag gggggaacag gccagagatg aaagtgcttc
     4381 atctgcatct cagtttatcc gtggactgaa acagaattac gcccagtaat tcacgatatt
     4441 ccattaatac catttcctga tgcccggcca ttgtgccggt ttttttatgg agtctgtatg
     4501 gcctattcag aggaacagcg tcctgaggcg caactcggta accagaatcg taacagcctg
     4561 aacattcagc aacccggcga aactgacagc tatgaagcat ttttctctga tccgaatcgc
     4621 tggaaggata acagtacgtc gttcagcctg ggcgatgtat tgccaacaat gggtaaaggt
     4681 ttcgcccagt ccgtccgggg aacaggggaa atggcccgtg gactcggtga tgcgatgatt
     4741 cagagcccgg taaaaacagg ggcgcgtatt ttaaatgagt tcagccgtat ggggctgccg
     4801 ggtgtcgcaa ctgtgcagga tatttttgcc ggtggcagca ggggggctga tgaggtcatc
     4861 gataccctgc ctgatggcaa aaacgcggtt actgatactg tcggtaaagg tctgaaggca
     4921 accggtaagg ctgtcagtga tggtgctaaa gccactgatg aatggctgac cggtaagatg
     4981 tcgccgggtg cagttcgtgc gctgaatacg ccgatgaccg aaggctatga tgattctgcg
     5041 gtctgggtgg cgaagggtgt aaacctgatt ggtgcgcttg tacctgatat ggttgctggc
     5101 ggtgtggcta aaaaggtggg tgatgtcaca ctgcgaaaaa tgctgaccgc cgggctggag
     5161 aaaaaataca tcgcggcagg gatgcagccg gaaagagcca cggcactggc agcagaagct
     5221 gtcgataaaa aaatgccgga tttattccag gcgggcctga tcacccattc cacagccagt
     5281 gcacaggggc agagtgcaat ggcggcagca gatgctgttc ttaatgcgga ttactctgag
     5341 ctggcgcagt caccgaaatt tcagcagacg tttttgtcca ttgacgccga cccgcagcac
     5401 gcacagctta ctgatcgcca gaaaatggat ctggcaaaag agcgtgttgc cgatgaggtg
     5461 cgcgcgcagc tggcaaccga tcctgaattg ctggctgtga atgccatggc ggcaaaactg
     5521 ggtgacgcac aactgtttaa tctggtgaca cgaggcacag cgaagaccgt taaaagcggc
     5581 attgtcagaa atgccacgga acagggggcg attaatgcgg cgcagggcgg ctattcacgc
     5641 tatcaggaaa acacggcatt gcgtgagacc gccggaatgg atgtgtcacc gtgggagggc
     5701 gtggctgacg caacgatcga aggtgcagcc tttggtgctg cgatgggggc tccatccggt
     5761 gcggttgccg gatatcgtgg cagacgtcag gccgcagaag aagcagccat gcgtgatgct
     5821 gaaaccgtgc agcaggacga cgcagccccg caaccagaat ctgttgatcc ggtggcgcag
     5881 catcgtgaat ccatgcaggg gatgaatcgc gagcagcttc tggagcagta tgctgatgcg
     5941 gatatggcaa cagagggtga cgcatccgca gctcatcgcc gggaagctgc cagccagctg
     6001 ttgaatgaac tggatgaaca ggcgaagcga caggcggtga tggatgagct gaaggcgaag
     6061 ccgcgtcctg aactgcttga ggaataccgc agactcagcc tgaaggaagg gcgtactgag
     6121 actgaagagc agcagttaca ggctatccgt gatgtgcttc gccctcagca ggaggccaga
     6181 ccggaggcac agccagaaaa tacagatgat ggcgacggga gtatttatcc gacggtgcgg
     6241 ttccgcgatc cggatgaagt gcgtattgaa attaacggga gtggcgcgtc cagaccagcg
     6301 gaacgcattg aaaaggtgcg tccggacaac cgttatttca cggatgagaa aagcgctatg
     6361 ggtagcgatg tttttcgcaa tgccagcgcc accggcctga aaccgtccgt agtgaagaaa
     6421 ggtgagaatc agtatgccgt tgaaatggat aatcctgcgt tctctgaaga tgtggcaacg
     6481 gaaaccatta acaccctggc tgacggagag cgtattactg atgctgatcc gatggagcag
     6541 ccagcgttca tgcgtgaccc gcgattccgg ggttttacgg gagatgatac agaagtgcag
     6601 gcccgccttg cccgtggcaa cgtgccgacg gcggaagagc tggtgcgttc acagatggct
     6661 gaaggtgatg ccggtccgac agctcaggag ttaactgagc gtccgcgtct gcccgccccc
     6721 ggcgatattc atcccggaca gggatatccg ttaccgggag aagtggcgcg tacgccggat
     6781 gaaaatcagg ccggacgtgg tggtcgtttt accataaccg gtgaggttaa gggccagagt
     6841 ttccagaaag gacgtgctcc ggcaccggaa aacgccgctg gtcgccaggg ggaaacactc
     6901 gagggtgaaa cggttcgtcg gggtctgccg tcaccggatg cgcagaacgc gacagcaccg
     6961 gtacgtgaag ggctaccggc tcctgacatt gcgcgtaatg ttcgtatgcc tcagcctgaa
     7021 tcacttcccc gcactgtacg ggactcactg cctgagctcg cacagcaggc agaagtacgt
     7081 cggcaggcca gagaaaatcg tgacatcctg cagcctgaga caatcgcacc tgaatctgaa
     7141 acaacagtct ctactgacag ggaagctacc gtgcgcggag gtgaagtcag gggcaaaaaa
     7201 attgaagact ttggtgaaga aattaagggc gcggcaaaac accgttatgc acagcttgcc
     7261 gaaacactgg gtaaaacgct ggaagacagg gattatgtca cgcaaccgct gagcaaactg
     7321 ttcccgaaac cggactacgc aaaactggcg aacgaaggtg ccgatgctga taccctggca
     7381 atgatagcgc tgtatcgtag cgatattccg gcgaagacga aactcaatgc agtgaagtgg
     7441 gtggagagtg taaagagtat ccgcacaagt gtggcgggaa tgctggaagg aaaagtgact
     7501 gccggacggc ttgctgagtg gatggaaggc agaatgcccg cccgttacgc ggacacatgg
     7561 caactgttac gcacgctgcc accctcgcag atagacaagg cttctgctta tcgggtggta
     7621 tccggtgtgt atcaggcggc aggagggaaa cgttacgatc cgccacggaa actttattca
     7681 ctgcgcaata aggacaataa ggggagcaat ctctttttct cggaaagcag ggatgaatta
     7741 ctgacaaagg caaaagcctg gtttgcagag caggaggaaa aatcacaggc gaaaggtgat
     7801 gaaaaaacag cactgtcacc ggatgacaaa atcagctttg acgtataccg gaatacccgc
     7861 agtggcgata tttttattgc ttacggcaaa aacaaaatgc gggtgagagg tggctttaag
     7921 tcagccagtg atgcgcgtaa gtacattgat tcacatcgtg atgagcttgt tcgtcatgtg
     7981 aaggagatgc gtgagatttc gcgtgaggag cagcgtaacg ccaccaaccg cgatcgtacc
     8041 gggccagaac gccgcaaggg tgatgtctca ccgaagcagt tcagcgatgc gtttggtttt
     8101 cgtggtgtgc agtttggtaa ctacgtggaa ggtccgcgtc gtcaggctga tttgaaccgg
     8161 gcttatgact cgctgcatga ccttgcggaa gtactgaatg taccgacaaa agcgctttcc
     8221 ctgaacggtc gtcttggcct ggcatttggt gcccgtggta agggtaaggc ggcagcacac
     8281 tatgagccgg gtgaggtggc aatcaacctg acaaaaggta acggaccggg tgcgctggcg
     8341 cacgaatggt tccattcact ggataattat tttggtcgtt atgatgtttc cactgacggg
     8401 aagattacgt caggtggtga ctttatgacg gaagccaggc gtgtcaagcg cgtatttaaa
     8461 gacggccggt ttgttgacgc tgaatatccg gtgcgtcagg aggtttacga cgcgtttaaa
     8521 ggtgtcattg aggccattaa aaacagtgac atgccgcgtc gttcagcgct tctcgatgag
     8581 gtgcgctcaa aaccgtactg gtcaacggat gttgaaatgg cggcacgtgc ctttgaacgt
     8641 tatgttcagg ataaagcgcg taaagctggc gtggagaatg attatctggt caatatccgt
     8701 aaggcacctg agcacaacac agataacacc tacgcttatc cgacgaatgc ggaactggat
     8761 ggcggtattc gtgaggcatt cgatcacctg ttccgcaccc tgaaaacccg tgagacggac
     8821 aagggcgttg cgttttattc ccgtaagggc gttacccgca cacctgaagg taatctcatt
     8881 tcggatgtta accgtagtgc ggaagccaaa ggcagcccgg tcccgcaggt tgaagcggtt
     8941 gcccgtggcg tgatgagcgg cattaaggac agtgacctga aggtccgtgt ggtgaagtca
     9001 cagaaagagg ctgaagcgct ggcgggtgaa ttgttcgatg gttacggcag ggtgcacgca
     9061 ttctatcgtc cggataaacg agaaattgtc ctggtggcgg ataacatccc tgacgggcgg
     9121 accgttcgcg agaagctgcg tcacgagatc attcaccatg ccatggagca tgttgtcaca
     9181 ccagcggaat atcagacgat tatcaaaacc gtgctgaaaa cccgcgacag tgataacgtc
     9241 accatccgtg aagcctggcg taaggttgat gcttcctatg gtaaggaatc accggaagta
     9301 caggcgggtg aatttctggc acatatggcg gagaaacagc cgaataaatt cgtggcggca
     9361 tgggagcgtg ttgttgccct ggtcaaaggg gtactgcgtc gtacggggtt actgaagccg
     9421 acggaactga acgatatcag acttgttcgc gagaccatcc gtacgttagg ccagcgtgtg
     9481 cgggaaggtt acacgccgcg tgaggatggc gcgggcgcat cgtctcagta ctcccgtagt
     9541 ggtaaacgtg atccgttcaa agtgccggaa ggtgagggcg agcgttatcg tgatgacctt
     9601 gccagaatga tgaaatctct gcgcaccaca gatttaacgg taaacatcgg gcgtacgccg
     9661 ccggtattgc gtcaccttgg tgcaccggat ttgccgctgg ttatttcccg cgatactgtg
     9721 cggaaggcca ccaatggtgt gaaacatgtg gtgccgatgg atgttatcga gagactaccg
     9781 gaactgatgc acgatccgga tgcaatttac cgctcagcga cagaaagaaa tgcggttgtg
     9841 atgctgcttg atgccgtgga taaaaatggt gatccggtgg tgtcagcggt acacatgaag
     9901 gctgtccggt cgcgtctgga aatcaacaag gtagcttctg tttacggtac agaaaatgga
     9961 aaaaaactga agagtatgga aatgaccggg ttaacgttgt accggagaga aaaattaagc
    10021 cgcgataacc ttctgcacag agggctccaa ttgcccaaag gggaacattc ttatcgcggc
    10081 tctgcggata aaatactcta tcctgaagat attcgcaagg ggccgtatta ctcccgtacc
    10141 agcagtctga caccggaaga gacaattgca tcgcgttttg tgcgccagat gcaggataaa
    10201 ttccaggtgc tgaaagctgt tcaggagaat atccgtaaaa ctggcggcaa agtggacgac
    10261 agtaacaacg cttatatggc ggaagaactc ttccacggga aggcggaaaa cgacctgaac
    10321 gtgatgaagg agcgctacgt tcagccactg gctaaattac tggcggacta caaaattgcg
    10381 caggccgatc tggatgagta cctctacgcc cgtcacgcgc cggaacgtaa cgcgcatatc
    10441 gcgaaaatca acccgaaaat gccggacggc ggttcgggga tgaccaacgc ggaagcggcg
    10501 gaaatcatgc agcgtgtacg taacagtggc aaacaggcac agtatgaccg tctggcaggg
    10561 attattgacg atatgctggc ccgtcgccgt gagcttatcc gtgaggccgg acttgaagag
    10621 aacggtgtgg tggatgcctg gcagaacgcc taccgttact acgttcccct gaaaggtcag
    10681 gatgttgacg gtgtggtgtc actgccccgt acaggtaagg gcttcaccat cggcggacgt
    10741 gaaagcaggc aagccatggg gcgtgcatcc cgggcacagt ctccgtccac tcaggcgata
    10801 caggacctga gcgaatcgct gatccgccat cgcaaaaacg aagtgggtaa cgccttcctg
    10861 aaactggtgc aggataatcc cgacaaggat tactggcagg tattcaccga tgacagaccg
    10921 gataccatgc ggacgattgc agagcgcaag gaccaggaaa ctggtgaaac cattcgcgaa
    10981 gttgtcgaac gccctgtacc gatggcaatg atggcagacc ggtacttcac caccaaaaag
    11041 aacggcaaaa cgtactacat caaactccat gatccgcgcc tgatgcgtgc gatgaagaat
    11101 atgggaccgg aaaccagcaa tgcctttgtt cgtacgctgg ggaaagttaa ccgcttcctg
    11161 gcaacggtga acacgtcgta taacccggaa ttcctggtca gtaacttcat ccgtgacgtg
    11221 cagacggcgg tgatgaacct gaaggcggag cagggaagga gcgacggtaa actgaaaggg
    11281 ctggataact tatccgccct ggctgtggtg aaagacagcc gttctgccat gtcagccgta
    11341 tacgccagtc tgcgtggtaa aaccctcacg ggaaaaggtg cacagtggca gaaggtgtgg
    11401 aaagagtttg ttgaggacgg aggtaaaacc ggctggttta acatgggtga ccttgaaggc
    11461 cagcagaagg aaatggatcg ccttgtatcg ctggcgaagg gaggatggaa aggccagagt
    11521 atcggtgcat ggaattcgtt ccttaacctt gtcgaggatg ccaacggggc ggttgaaaac
    11581 gctctgcgtc tttctgccta taaacacgcc cgtgatgccg gtttgtcacg ccagcaggcg
    11641 gcgtctcttg ccaaaaacat gacggtgaac tttaaccgtc gtggtgagca gggagcgctg
    11701 atgaactcgc tgtacatgtt cgccaacgcc agcattcagg ggaccgcaaa cctggtgaga
    11761 acgctcggac atcttaatgg cgacgggccg ttactggagc gccttcgctg gaagaatctc
    11821 aatgtaccgc agaaaatcgc gcttgcagct gtgggagcgg gttatctgct tggctcgctt
    11881 aaccgcagtg ttgcggggga ggatgatgac ggggttaact ggtatgacaa ggtgccgtct
    11941 catgtgaaag agcgtaacct cgtcattatg aaatcggtgt tcgggggcaa ggccggagag
    12001 tactggagta ttcctctgcc ttacgggtac aacgttttct tcctgcttgg tcacacagcc
    12061 gaaggggtgg cagcgggcga cctgaccgct tcacgtgctg ccggtaatgt tgttggtggt
    12121 gtgcttgggg cattcagtcc tgtgggcagt gaaacgtcgg aaacactgtc cggggcattg
    12181 ctgaaaaatg cagcaccgac aattctgcgt ccgtttgcga atcttgccat gaacgaaaac
    12241 ttcatggggg cgcagattta ccaggagaac atgccgtttg gtacaccaaa acctgacagc
    12301 cagctgggaa gacgttcaac gccagaagcg tacaaggcgt ttgcatcctg gctgaatgcg
    12361 ttctctggtg gcagccagta ccgttccggt gcggtggatg tcacgccgga atcactgaaa
    12421 tactggattg actatatctc cggagggaca gggcgcttca tttccaaaac cacggatgcg
    12481 gcggtgaaat cgctgaacgg tattgatata ccggagcagc aggtgccctt cctggggaaa
    12541 atttcaggtg aggtgatgcc gtatgctgac cagcagaaga tgtacgaccg gatgacagag
    12601 gttgcgcagt atcacgcaga gctgaagagt ctgaccggtg cagaaagaac ggcgttcatt
    12661 gacgagaaca acggaaaatt gtcgatgaac gggcttatgc aggatacccg gaagagactg
    12721 aaggatttgc gtaaacagcg tgatgccatt tacgccgaca gtactctcag tctggcgcaa
    12781 cagtcggcga tggtgaaatc ggttgagcgg gatatgaaaa ttgccgtgga tcggtttaac
    12841 cgcgagtaca acaaaaaagt gggagtggag taaagaacat gccccgtacg gaagtgcggg
    12901 gcatgcttaa cggggtgtgt acataaatgg gtactgatat gacgattatt tcgccacttt
    12961 ctgtatttaa atcaatgact taatacttat attcattata tccatttaac taagaggaca
    13021 atgcggcatg agtatacccg ctaatggact gcggggtaag tacgctgccg ctcgattgct
    13081 taaaccctcg ccatttatgc cgggttttta tcatttttct taatgttttc cgcacgttct
    13141 gctttttggc gtgcttctgc tttgcgcttg ttgctcatgt cgttacggat ctgtgcatga
    13201 ctcattaacg cgaagataaa ggtgccgccg cagatgttcc ccgctaaagt aggtagtgcg
    13261 aagggccaga tgaaatcgct ccagtgcagc gtaccgttaa acaccagata gaggatttca
    13321 acagaaccga caacgatgtg agtggtgtca cccagggcaa taagccaggt catcaatata
    13381 atcaccacaa tctttgccgc acccgctgca ggaaacatcc ataccatagt ggcgatcagc
    13441 cagccggaaa tgatcgcgtt ggcaaacatc tcgctggggg tgttcttcat cacatccatg
    13501 ccgattttga caaatgcatc gcgagtttct tcattgaaga ttggcatata ttcaaatgcc
    13561 cacgccgcaa tacctgtccc gagaatatta cccagcagca cgacgcccca taaccgcata
    13621 agtaagccaa cgttgctcat tgtcggtttt tgcatgacgg gtagtaccgc agtcacggtg
    13681 ttttcggtaa ataattgctg gcgggccata atgacgataa taaaaccaaa ggtataaccg
    13741 agattctcca gcaagaagct gcccggcaca ccttccagtt cgacatgaaa tatccctttt
    13801 gccagtaatg aagcgcccat cgacagaccc gccgcaatgg ctgaccacag tagtgccatt
    13861 gcgtcgcgtt ccagctcttt ttcaccatcc tggcggatat gctcatgaat tgccatcgcc
    13921 cgggagggga gtcgatcttc atctatttct atttttttgc cacgctcttt ttcttcgctc
    13981 tcaacttcaa tttcgtcgct gtgttgatca attttgtcgt tgtccatggt ctcttctctt
    14041 gaattagcac gtatagctaa agcgtagcgg cttttttgct cgcaactggc gggagttact
    14101 ttgaaaatgt agaaaaggcc gcgtttgcct ttttctgttt ctatagaatc aagtagccta
    14161 cagggcggcg attaccaggc tatgatcaaa tcagcaaatc agggcgtctg gacatcagtt
    14221 gacgtgctgt tacaatcgcc cacacctaaa caggcggata cggtatcgtt ccgtcatgga
    14281 tggcaaactg cataagccat aaaaaaacag ggagacattt atgaagcttc gcctgtcggc
    14341 gcttgctctg ggaactacgc ttctggtggg gtgtgcgagt tccggtacag atcagcaagg
    14401 gcgttctgac ccgttagaag ggttcaaccg caccatgtac aactttaact tcaatgtatt
    14461 agacccgtat attgttcgac cggtcgctgt cgcctggcgt gattatgttc cgcaaccggc
    14521 gcgtaacggt ttgagcaatt ttaccggcaa ccttgaagaa cctgcggtga tggttaacta
    14581 cttcttgcag ggcgaccctt atcaggggat ggtccacttt acccgctttt tcctgaacac
    14641 cattttgggg atgggcggct ttattgatgt tgcagggatg gcgaacccga aactgcaacg
    14701 gactgaacct caccgcttcg gtagtacgct tggtcattat ggcgtgggtt atggcccgta
    14761 cgttcaatta ccgttctacg gtagcttcac gctgcgtgat gacggtggtg atatggcgga
    14821 tggtctttac ccggttcttt cctggctgac ctggccgatg tctgtgggta aatggacgct
    14881 tgaaggtatc gaaacccgcg cacagctgct ggattccgac ggtctgctgc gtcagtcgtc
    14941 cgatccttat attatggtgc gcgaagcgta cttccagcgt catgatttca tcgctaatgg
    15001 cggcgaactc aaaccgcagg aaaatccgaa cgcgcaagcg attcaggatg atttaaaaga
    15061 tatcgattct gaata
//
LOCUS       DBFIFM010000115        14761 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.115, whole genome shotgun sequence.
ACCESSION   DBFIFM010000115 DBFIFM010000000
VERSION     DBFIFM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.115"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            152..1288
                     /locus_tag="N7C74_002990"
                     /pseudo
     CDS             152..1288
                     /locus_tag="N7C74_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            1282..1419
                     /locus_tag="N7C74_002991"
                     /pseudo
     CDS             1282..1419
                     /locus_tag="N7C74_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
     gene            complement(1421..2032)
                     /gene="emtA"
                     /locus_tag="N7C74_002992"
     CDS             complement(1421..2032)
                     /gene="emtA"
                     /locus_tag="N7C74_002992"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HGU2432820.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            2132..3046
                     /gene="ldcA"
                     /locus_tag="N7C74_002993"
     CDS             2132..3046
                     /gene="ldcA"
                     /locus_tag="N7C74_002993"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HGU2432821.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNTFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILTNTREGTQLTISGHPVLKM"
     gene            3142..4878
                     /gene="cvrA"
                     /locus_tag="N7C74_002994"
     CDS             3142..4878
                     /gene="cvrA"
                     /locus_tag="N7C74_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HGU2432822.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(5267..6337)
                     /gene="dadX"
                     /locus_tag="N7C74_002995"
     CDS             complement(5267..6337)
                     /gene="dadX"
                     /locus_tag="N7C74_002995"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HGU2432823.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEMYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(6347..7645)
                     /gene="dadA"
                     /locus_tag="N7C74_002996"
     CDS             complement(6347..7645)
                     /gene="dadA"
                     /locus_tag="N7C74_002996"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HGU2432824.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8008..9540
                     /gene="ycgB"
                     /locus_tag="N7C74_002997"
     CDS             8008..9540
                     /gene="ycgB"
                     /locus_tag="N7C74_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HGU2432825.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            complement(9592..10311)
                     /gene="fadR"
                     /locus_tag="N7C74_002998"
     CDS             complement(9592..10311)
                     /gene="fadR"
                     /locus_tag="N7C74_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HGU2432826.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            10533..12074
                     /gene="nhaB"
                     /locus_tag="N7C74_002999"
     CDS             10533..12074
                     /gene="nhaB"
                     /locus_tag="N7C74_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HGU2432827.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            12220..12750
                     /gene="dsbB"
                     /locus_tag="N7C74_003000"
     CDS             12220..12750
                     /gene="dsbB"
                     /locus_tag="N7C74_003000"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HGU2432828.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(12796..14064)
                     /gene="umuC"
                     /locus_tag="N7C74_003001"
     CDS             complement(12796..14064)
                     /gene="umuC"
                     /locus_tag="N7C74_003001"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HGU2432829.1"
                     /translation="MFALCDVNAFYASCETVFRPELWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(14064..14483)
                     /gene="umuD"
                     /locus_tag="N7C74_003002"
     CDS             complement(14064..14483)
                     /gene="umuD"
                     /locus_tag="N7C74_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HGU2432830.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT         3478 a         3843 c         3688 g         3752 t
ORIGIN      
        1 tgattttgcc cggtaataga gaattattct cagagaagct catcatgatt gcaccataca
       61 aatatcatga tgataagagg atattattca ttgacttaaa caaaatgaca aaatccatag
      121 acccatagcc tgggtactat ataggagtgg tatgggcatc aaacaacaca atgggaatac
      181 caaagccgat cgtctcgctg aattaaaaat ccgttcgccc tcaattcaac tgataaaatt
      241 tggcgctatt ggtttgaatg caattctctt ttcccccctg ctgatagctg ctgatacagg
      301 aagtcaatat ggcaccaata ttactattaa tgatggtgac agaattactg gagataccgc
      361 cgatccatca ggaaacctct atggtgtaat gaccccagca ggaaacacgc ctggcaatat
      421 caacctgggt aatgatgtca ccgtcaatgt caacgacgcc tctggatatg caaaaggaat
      481 cattattcag ggcaaaaaca gctccctgac agctaaccga ctcacagtag atgttgttgg
      541 tcaaacctct gccatcggca ttaatttaat tggtgactat acccatgctg acttaggcac
      601 aggcagcacc attaagagta acgatgacgg catcattatt gggcatagct caacactaac
      661 agccactcaa ttcaccattg aaaactcgaa cggtataggc ctaaccatca atgactatgg
      721 caccagtgtc gatcttggaa gcggaagtaa aatcaagacc gatggaagta caggtgttta
      781 tatcggtggt ctcaacggca ataacgccaa tggtgctgcg cgttttacgg cgacagacct
      841 gacaatcgat gttcagggct acagcgccat ggggataaac gtacagaaaa actctgttgt
      901 cgatctcgga acaaacagtt ccattaaaac cagtggcgat aatgcacacg gcctctggag
      961 ctttggccag gttagcgcga atgcactcac tgttgatgta actggagccg cggccaatgg
     1021 cgtcgaagtt cgtggtggta caaccactat cggtgcagat agccatattt cttccgcgca
     1081 gggcggtggt ctcgtcacca gtggttcaga cgcgacaatc aatttttctg gcacggcagc
     1141 gcaacgaaac agcatctttt ccggcggttc ttatggtgcc tcggcccaga cggcaacggc
     1201 tgttatcaac atgcaaaata ccgatattac ggttgatcgt aatggcagtc tggcgctggg
     1261 tttgtgggcg ctcagcggcg caagaatgaa accatcacca ctccccgtct gagcttccgt
     1321 tttcttaacg tcagcccgac ggtggagcgg caattacagc ggattatttt ctctctcgag
     1381 cgggaagccc gggagaaagc ggacaaagta cgcgactgat ttacatcgcg tccagtgcct
     1441 gctcaagttt gtagatatag cgcggagcct gcggcgcagg atgatttcgc gctacgtgat
     1501 cgaggaactc gtcagcatcc agatcgttga ttttgctgat tgcctttttc cgatctgacg
     1561 agaaagtccg tagcagcgca cctgccccgt tagcatatga caccaccagc gcatattgca
     1621 gtaccttcgg atcttcaatg cctgccagcg ggccggtttc cagaatattc aggtaagccg
     1681 cccccataga aatattacgc tccggatttt tcagctcgct ggtcgtcggc tcaccactcc
     1741 agcccatacg ccgataaaca tcacgtccgg aggttgaagc ttttaactgc atcaaaccaa
     1801 tggcattcga tttactcacc gcgttcggat taccacccga ttcgatagcg ataatcgccg
     1861 tgatcaattg tggatcgacg ccccaggctg caccggcttt ctggcttatt ggcatccact
     1921 gcatcgcacg ttgcaccgga actttcgcgt tccacggcgg gttcgtatag tcatgctttg
     1981 atgaacaacc cgctaataac acaatcaaaa aggcaaacca tctcaatttc acttcttcta
     2041 tccttatagc ctggaatcgc cgttgaggtg acaacggcac acctaagcgg catgatatac
     2101 atttcgtatc tgttgtcagc aaggaattgc catgtctctg tttcacttaa ttgccccatc
     2161 gggttactgc attaaacagc acgccgcgct gcgtggtatc cagcgcctta ccgacgcggg
     2221 gcatcaagtc aataacgtcg aggttattgc ccgtcgttgt gagcgttttg cgggtacgga
     2281 aacggagcgt ctggaagatc tcaattccct tgccagactg actaccccca acaccatcgt
     2341 gctggctgta cgcggcggtt acggtgccag tcgtttactg gcagatattg actggcaggc
     2401 actggtggct cgccaacaat atgacccgtt gctcatatgc gggcatagcg attttaccgc
     2461 cattcagtgc ggtcttctgg cgcaaggcaa tgtcatcacc tttagcggcc ccatgctggt
     2521 agcaaacttt ggcgcggatg aactgaacac ctttaccgag caccatttct ggctggcgtt
     2581 acgcaatgaa acctttacca ttgaatggca aggggaaggc ccgacatgtc gggccgaagg
     2641 cacgttgtgg ggaggcaatc ttgcgatgct gatttcactg atcggtacac cgtggatgcc
     2701 aaaaattgag aacggtattc tggtgctgga agatattaac gagcaccctt tccgcgtcga
     2761 acgtatgctg ttgcagctct atcatgcggg aattttgcca cgtcagaagg cgattattct
     2821 cggtagcttt agcggcagta cgcctaatga ttatgacgca ggttacaacc tggagtcagt
     2881 gtacgcattt ttgcgttccc gcctgtcgat tccactcatt accggcctcg attttggtca
     2941 tgaacaacgc acggttacgc tgccgttggg cgcacatgcc attcttacta acacccggga
     3001 aggcactcag ttgacgatta gcggtcatcc tgttcttaaa atgtaaaaaa aatcacgctt
     3061 cagggctaag taaaacgctg tctctgccgc taatatggta gggtttatat aagagacagc
     3121 gtaatcagga gtaaccgacc gttggatgcc acaacaataa ttagcctttt catcttagga
     3181 tccatccttg ttaccagcag tatattactt agttcatttt cttctcgtct tggcattcct
     3241 attctggtta tttttctggc gatcggcatg ctggcgggag tcgatggcgt cggcggtatc
     3301 ccatttgata attacccctt cgcctacatg gtaagtaacc tggcgctggc gattattttg
     3361 ctcgatggcg ggatgcgcac tcaggccagt tcctttcgtg tggcgttagg accggcgctg
     3421 tcgctggcga cgctgggcgt acttatcacc tctggtttaa ccggcatgat ggcggcgtgg
     3481 ctgtttaatc ttgatttgat tgaaggctta ttaatcggcg ctatcgtcgg ctccaccgat
     3541 gctgcagcgg tcttttctct gctgggtggt aaggggctta acgaacgtgt tggctcgacg
     3601 ctggaaattg aatccggcag taatgatcca atggcggtct ttctgacgat taccctgatt
     3661 gcgatgatcc agcagcatga aagcagtgtc agttggatgt tcgtggtcga tattctgcag
     3721 caatttggcc tcgggattgt catcggtctt ggcggtggtt atttactgct gcaaatgatt
     3781 aatcgcatcg ccctgcccgc cggtttatat ccattgctgg cattaagcgg cggtatttta
     3841 atttttgctt taaccacggc gctggaaggt agcggtattc tggctgttta tctgtgcggt
     3901 tttctgctgg gtaatcggcc gattcgcaac cgctacggca tcctgcaaaa tttcgacggc
     3961 ctcgcctggc tggcgcaaat cgccatgttc ctggtgctgg ggctgttggt taacccaagc
     4021 gatctgctgc ctattgccat tccggcgctc attttgtccg catggatgat tttcttcgcc
     4081 cgtcctcttt cggtatttgc cggattgcta ccattccgtg gcttcaatct gcgtgagcgc
     4141 gtgtttatca gctgggtggg attacgcggc gcggtgccga ttattctggc cgtgttcccg
     4201 atgatggcgg ggctggagaa tgcacgtctg ttctttaatg tcgccttctt tgtggttctg
     4261 gtttcactgc tcttgcaggg aacatcactc tcgtgggcgg cgaaaaaagc caaagtggtg
     4321 gttccgcccg tcggacgtcc ggtgtcacgc gttggcctgg atattcatcc ggaaaatccg
     4381 tgggagcagt ttgtttatca attgagtgcc gataaatggt gcgtgggcgc ggcactgcgc
     4441 gatttgcata tgccaaaaga gacgcgtatt gcggcactgt ttcgtgataa ccagttgctt
     4501 catcctaccg gcagcacccg actgcgcgaa ggcgatgtgt tgtgtgtaat tggtcgggaa
     4561 cgcgatctcc cggcgctcgg taaactgttc agccagtcgc cgccggtcgc gctggatcaa
     4621 cgcttctttg gtgacttcat tctcgaagcc agcgccaaat atgcagatgt ggcgctgata
     4681 tatggcctgg aagacgggcg agaatatcgt gataagcaac aaacgctggg tgaaattgtc
     4741 cagcagttgt taggcgcggc accggttgtc ggtgaccagg tagagtttgc cgggatgatc
     4801 tggacggtgg ccgagaaaga agataatgaa gtgttgaaga ttggcgttcg agtggctgaa
     4861 gaggaagctg aatcttaaat ttaatgaagc atctatcgcc ggttacgatg ctttgcttaa
     4921 cgcagcctga gcctgtatat aaacttgtca tttcctgatt ttgatatgca cattccaaca
     4981 tcagaagaag cctgttaatc aacattctga acatgaaccc gaatctgcat caagggtgaa
     5041 gcgtgattta tctgaagtcg agttcgagtc aacaatgaag cgtaagcgtt ttatgtagaa
     5101 cgcctgtgcg gtgagttcag gttccgggga aatagtgtcc atccttttaa tgggcactat
     5161 catgggcaat ggctctgtca cattaacaac tacagttgct gaccagccgg atggatgatt
     5221 ttttttgcaa ccagaagacg ttgcctccga tccgacttac aacaagttac accgtcacaa
     5281 ccgggacgcg taacgccagc gcgcacatca actcatagcc caccgttccg gcagcggcgg
     5341 cgacatcatc aattttgatc tccttgcccc acagctcaac tggcgtacca atacccgcct
     5401 gcgggcaagg cgttaaatcg accgccagca tatccatcga gactgtcccc accgtcatgg
     5461 tgcgcacgcc gtccactaaa acaggggtac cggtaggcgc gtggcgcgga taaccgtcgg
     5521 cgtatcctgc ggcaacaatg ccgattcgct gctcatcgcg cgcagtataa cgaccgccgt
     5581 agcccacacg ctcgcccgct tttagcgtct ggacaccaat aatctcactg cttagcgtca
     5641 tcaccggacg taatccagta ttggcgatat cacgccactg accggacggc gaagcgccat
     5701 acaaaataat gccaggccga acccagtcaa aatgcgcttc tgggtgccac agagtcgccg
     5761 ccgaattgga caacgaacgc cgacactcca gcccttccgc cgcctgctca atacgcgcca
     5821 tcgcgccgga aattccatca ggatgttccg cctcggcaaa atgcgacatc agggtcattt
     5881 cgccaacatt cgccatcgcc cgcaactgct gccagacggt aagcacgcga tcgggctgga
     5941 agcccaaccg attcatccca ctgttcactt taagataaat atccaacggt gcttttagcc
     6001 gcgcattttg cagtgctttg agctgccagt tgctgtgtac gcaggtggtc aggcggtgct
     6061 ggtcatacat ttccagatcc tgagcatgga aaaatccttc cagcatcagg atcggtcctt
     6121 tccagccgcg ctcacgtaac gttattgctt cttccaggtt aagtaatgca aagccatcgg
     6181 tagccccgag cgcgctccag atacgctcaa taccgtgccc gtaagcgttc gcttttacca
     6241 ccgaccagac gcgcgcgtgc ggcgcggcct ggcggacaat ggacagattc tgttttaatg
     6301 cctgcagatc gaggctggcc tgtatcggac gggtcatctc gtttccttag ctgtgtgcgc
     6361 catgtaaatg gcccggacgt gatggcgtaa atccacggct gtagcgcgct acgcttagat
     6421 cctcatatgg gatcgctggc gtgcgaccag agagcagatc gcttaacaac tgaccggaac
     6481 cgcaagccat cgtccagccg agcgtgccgt gaccggtatt tagccacaga tttttaaagc
     6541 gtgtacgccc gacaaccggc gtgccgtctg gcgtcatcgg gcgcagaccc gtccagaaag
     6601 tcgcctgctc gacatgaccg ccgcgtggat agagatcgcg aaccaccatc tccagcgttt
     6661 cacgacgcgg ttgcaacagc tcggtattaa aaccaacaat ttccgccatt ccgccaacac
     6721 gaatgcggtt atcgaaacgg gtaatggcga ttttgtaggt ttcatcaaga atggtggata
     6781 ccggcgcacc atcttcctgc gcaattggaa tggtcagcga gtagcctttc agcggataca
     6841 ccggaatatc aacaatgcct ttgagcatcg ccgtcgagta agaaccaaac gccatcacat
     6901 acgcatcggc cttaatcact tcatcgccac acttcacgcc gtagatttgc tcgccgtcgc
     6961 aaagcagttg gtcaacgggc gtattaaagc ggaatttaac ccccgcctgc tccgccatcc
     7021 gcgccagatt ctgggtaaac agctggcagt ctccggtttc atcattgggc aattgcagac
     7081 cacccgtcag tttgtgcgct acttctgcca gcgcgggctc cacttccgcc aggcggctgg
     7141 attccagcag ctgatacggt acgccggcat cttccagcac ggcgatatcg cgggtcgcat
     7201 tttcatactg ttgttcggta cggaacagtt gcagcgtccc accctgacgc ccttcatact
     7261 gaatattggt ttcggcgcgc aatgctttca ggcaatcacg gctgtattcc gccagacgca
     7321 ccatccgccc tttgttttcc atgtagtggc tggtgtcgca gttacgtaac atttgccaca
     7381 tccatttcaa ctggaactgc gtaccgtcga gacgaaccgc cagcggcgca tggcgctgga
     7441 acatccattt aatcgctttt aaaggcacac ctggtgccgc ccacggtgca gcatatccgg
     7501 gggagatttg cccggcattc gcggcactgg tttccagggc ggcccccggc tcccgatcaa
     7561 tgacggtgac ctcatgtcct gcctgattta agtaccaggc gctggcaacg cctaccacac
     7621 cacttcccag tatgacaact cgcatagcca ctccgataac ggtagataca gtaaaagaat
     7681 aataatctaa ttacatcttg ataactcagt tgaaaatatt attcaacata cggctttttt
     7741 atggtgagct ggctcacatc tccctgactc tgcggcaata tggcgtcttt agtatctcct
     7801 gctgaacgcc ctttttatcc agcataaaat tccgttcaga aacggattag tggcactctg
     7861 ataataagaa atcgcaaaga aaaaaattct ccggcgacgc ggtttttaac aggtgttcta
     7921 tgcttgaaat gaggtgctcc acacagagag cgccgacaac aatgaggtgc tccacacaga
     7981 gagcgccgac aacaatgagg tgcgcgtatg gcgacgatcg attctatgaa taaggacacc
     8041 acacgtttga gcgatggacc cgactggacg ttcgacctgc tggatgttta tctggcagag
     8101 atagaccggg tggcgaaact ctaccggctg gatacctacc cgcaccagat tgaagtgata
     8161 acctcagaac agatgatgga tgcctactcc agcgtcggca tgccaattaa ctatccgcac
     8221 tggtcattcg gtaaaaagtt tatcgagact gaacggctgt ataagcacgg tcagcaagga
     8281 ctggcctatg aaatcgtcat taactctaac ccgtgtatcg cttacctgat ggaagagaac
     8341 accattacca tgcaagcgct ggtgatggct catgcctgct atgggcataa ctctttcttc
     8401 aaaaacaatt acttattccg tagctggacc gacgccagtt cgattgtcga ttatctgatt
     8461 tttgcccgta aatatattac cgagtgcgaa gaacgttatg gcgttgatga agtagaacgg
     8521 cttctggact cgtgccacgc gctgatgaac tacggcgtgg accggtacaa acgcccgcaa
     8581 aaaatctcgc tgcaagaaga gaaagcccgg cagaaaagtc gcgaagagta tttgcaaagt
     8641 caggtcaata tgctctggcg taccctgccg aagcgcgagg aagagaaaac ggttgctgaa
     8701 gcgcgccgtt atccgtccga accacaagaa aacctgctct attttatgga gaaaaatgcg
     8761 ccactgctgg aatcatggca gcgtgaaatc ctgcgtattg tgcgtaaggt gagccagtat
     8821 ttttatccgc aaaaacagac tcaggtgatg aacgaaggct gggccacctt ctggcactac
     8881 accatcctta accatctgta tgatgaaggg aaagtaacgg aacgttttat gctggagttt
     8941 ttgcacagcc acaccaatgt ggtcttccag cccccctata acagcccgtg gtacagcggc
     9001 atcaacccgt atgccctcgg gttcgccatg ttccaggata ttaaacggat ttgtcagtcg
     9061 ccaacggaag aagacaaata ctggttcccg gatatcgccg gttccgactg gctggaaacg
     9121 ctgcatttcg cgatgcgtga tttcaaagat gagagtttta tcagccagtt cctgtcgccg
     9181 aaagtgatgc gtgatttccg cttcttcacc gtgctggatg atgatcggca taattatctg
     9241 gagatttccg ctattcataa tgaagaaggt tatcgggaga tccgtaaccg gctatcgtcg
     9301 caatataact taagtaatct ggagccgaat attcagatct ggaacgtgga tttgcgcggc
     9361 gaccgttcgc tgacgctgcg ttacattcca cataatcgcg caccgctgga tcgggggcgc
     9421 aaagaagtac tgaagcatgt gcatcgcctg tggggatttg atgtgatgct ggaacagcaa
     9481 aacgaagacg gcagcgtcga gttgctggaa cgttgcccgc cgagaatggg aaatctgtaa
     9541 aaacaacaaa aaacccctcg tttgaggggt ttgtttttta aacggaaggg attatcgccc
     9601 ctgaatggct aaatcacccg gcagattttt ctgcatccgg tgccaaatct cgccactctc
     9661 atgcccatag cgacgcactg tttcgtacac ctgatcgtgc gcgccttcac tgcacaacgc
     9721 cgacagtttg tggtagaagc ccagcgccag actgcgcgct tccggattgg cgaaatagtg
     9781 acgaccaatg cgcgtataca gtcccttcat cccgttaagg atcagaccgt aaatcgggtt
     9841 gccggaagca aacgccaggc cgcggaatat gttgtaatcc agctcggcaa aggcatcggc
     9901 gtgatcggcc acttcattag cggtagccag cacttcctgc gctttatcgg gatgctgacg
     9961 aaacgcggtg cgaataaaaa tagttgaaat attggtacgc accgacagca aattatcaat
    10021 aagctgcggc acactttcgt gatccagtcg cgccagtgtt tcaaggatat ttaaaccaga
    10081 ggtttcccag aaattattca ccttcgtcgg cttgccatgt tgaatggtca accagccatc
    10141 tcgtgccaga cgctgtaaca cttcacgtaa cgtagtacgc gttacgccaa ttaattctga
    10201 aagttcacgt tctgcgggca aaatagtccc gggagggaag cggttattcc agatactttc
    10261 aataatgtac tcttccgcga accccgccgg gctttgcgcc ttaatgacca tagtgagatt
    10321 tccataacac agcaaaacaa agttggactc atcataccag agggctaact acgctgatag
    10381 cagacaaaaa gaagaaaaaa gggatcaagg cttcattttc cgcatctctt tccccttgac
    10441 tatcaagact tgcctcactc ctgcctgacc gttaagcttt gcgtttaaac ataaaaacat
    10501 cattgattat ttgtaaggta agggaaacca tcatggagat ctcctggggc cgcgcgctat
    10561 ggcgcaattt tttgggccag tcccccgact ggtacaaact cgccctcatc attttcttaa
    10621 tcgtaaatcc gttaattttc ctcatcagcc ctttcgtcgc tggctggttg ctggtagcgg
    10681 aatttatttt cactctggcg atggccctga aatgctaccc gctgctcccc ggtggcctgt
    10741 tggctatcga agcggtattc atcggcatga ccagcgcgga acacgtccgt gaagaggtgg
    10801 cggcaaatct tgaagtcttg ctgttactga tgtttatggt ggcgggtatc tattttatga
    10861 aacagctatt gctgttcata tttacccgtt tgctgttaag cattcgctcc aaaatgctgc
    10921 tgtcgctctc tttttgcgta gcggcagcgt tcctctccgc gttcctcgat gccttaaccg
    10981 tcgtggcggt ggtgatcagc gttgccgtcg gtttttatgg tatttatcat cgcgtagcct
    11041 cttcccgtac cgaagacacc gacctgcaag acgatagtca tatcgacaag cattacaaag
    11101 tggttctgga acagttccgt ggctttctgc gtagcctgat gatgcatgcc ggtgtcggca
    11161 ccgcattagg cggcgtaatg accatggtgg gcgaaccaca gaacctgatc atcgctaaag
    11221 cggctggctg gcattttggc gatttcttcc tgcgcatgtc gccggtgacc gttccggttc
    11281 tgatttgtgg cctgttaacc tgcctgctgg tagagaagct gcgttggttt ggctacggtg
    11341 aaacgctgcc ggagaaagtc cgcgaagtgt tgcaacagtt tgacgatcaa agccgccacc
    11401 agcgtacccg tcaggataaa atccgtctga ttgtccaggc gattattggc gtctggctgg
    11461 tgactgcgct ggcgttgcat ctggcggaag ttggcttgat tggtttgtca gtcattattc
    11521 tggcaacatc attgaccggt gtcaccgatg aacatgctat cggtaaagcc ttcaccgaat
    11581 ctctgccatt caccgcactg ttgacggtgt ttttctcggt agtcgcggtg attatcgacc
    11641 aacaactgtt ttcgccgatt attcagtttg tgttgcaggc atcggaacat gctcagctgt
    11701 cgctgttcta tattttcaac ggtctgctgt catccatttc ggataacgtc ttcgtgggga
    11761 cgatttatat caacgaagcg aaagcggcaa tggaaagtgg cgctatcacg ttgaagcaat
    11821 acgagctgct ggcggtcgcc attaataccg gtaccaatct gccctctgtc gctacgccga
    11881 acggtcaggc tgcgttcctg ttcctgctga cctctgcact cgcgccattg attcgcctct
    11941 cttatggccg catggtgtgg atggccctgc cttacaccct cgtcctgaca ctcgtcggac
    12001 tgctctgcgt cgagtttacg cttgcccctg taaccgaatg gtttatgcaa atgggctgga
    12061 tagcaacgct ttgataacaa cttaccgggc atataaatgc ccggtttgcc ttttcgcccg
    12121 ataattgtcc aattgcgtcc tatttttgct gcctcctggt ggcgcagcga atgaattggt
    12181 ttaaactgcg cactctatgc atattgcagg gaaatgatta tgttacgatt tttgaaccaa
    12241 tgttcacaag gccggggcgc gtggctgttg atggcgttta ctgctctggc actggaactg
    12301 acggcgctgt ggttccagca tgtgatgtta ctgaaacctt gcgtgctctg tatttatgaa
    12361 cgctgcgcat tattcggcgt tctgggtgct gcgctgattg gcgcgatcgc cccaaaaacg
    12421 ccactgcgtt atgtagcgat ggttatctgg ttgtatagtg cgttccgcgg tgtgcagtta
    12481 acttacgagc acaccatgct tcagctctat ccttcgccgt ttgccacctg tgattttatg
    12541 gttcgtttcc cggaatggct gccgctggat aaatgggtgc cgcaagtgtt tgtcgcctct
    12601 ggcgattgcg ccgagcgtca gtgggatttt ttaggtctgg aaatgccgca gtggctgctc
    12661 ggtattttta tcgcttacct gattgtcgcg gtgctggtgg tgatttccca gccgtttaaa
    12721 gcgaaaaaac gtgacctgtt cggtcgctaa tccattcggc gctcctgcgg gagcgctttt
    12781 ttcctgccgc tgtgtttatt tgacccgcag taaatcagaa cttcgcgttg tataacgtgg
    12841 tgaaagcatg gctcgcttca tctgccattg ttgctggatc ccctgcccgg caaaatagag
    12901 tgttcctctg ccctctttag cattcagtgt atccattacc gccatcaatt gctcactccc
    12961 ggggcgcgga gcgttgtcat cgaataaatt gagctgcgcg actccctgac tgaagaaatc
    13021 ccccagcatc acgcccgctt tttggtagcg atggcccgct tgccagatgg catccagaga
    13081 tcgcgtagcg gcgttaatga tatccctgct gtcctgagtg ggcgtcagca gttttaccga
    13141 cgcgctattg ccgtaataag gttcattgag cgcaaatggt gacgtcttaa taaatgtgga
    13201 gataaaccga caatattgat gctcactgcg aagtttttcc gccgcccggg cagcgtaact
    13261 acaaatggcc tgccgcatcg acgtataatc cgtgatgcgt tcaccaaacg agcgggaaca
    13321 gataatttcc tgctttgtcg gtgcaaattc ttccagttgc aaacagggtt caccgcgcag
    13381 ttcacgcacc gttctttcga gcacgacatt aaaatgttta cggataaacc ggatatctgt
    13441 atccgccaaa tcgagaacgg ttttgatccc catcgcgtcc agttttttgc tgatccgccg
    13501 tccaatcccc cagacgtcat ccacggggag agcagacatt aatttacgct ggcgttccag
    13561 atttgataaa tccaccaccc cacccgtctg ccgctgccat ttttttgccg catgattggc
    13621 aagcttagcc agcgttttgg tctgggcgat ccccacacca acagtaagat gggtacgttg
    13681 tagcaccgtt gcgcgaattt ctctgccaaa gtcagtcaga tcgcgacaat tacgcacacc
    13741 tgtcagatcg cagaatgcct catcaatact gtaaatctcg acgcggggcg atagttcttc
    13801 cagcgtcgac atcacccgat tgctcatgtc tgcgtaaagc tcataattgc tgctaaagca
    13861 aaccacgcca cagcgacgaa acagatcttt ttgtttgaac cagggatcgc ccattttaac
    13921 gccaagcgcc tttgcctcag cgtttcgggc gataacgcaa ccgtcattat tcgatagcac
    13981 aaccaccggt ttaccccata actcagggcg aaacaccgtc tcacagctgg cataaaacgc
    14041 gtttacatca cagagggcaa acatcagcgc atcgccttaa cgacgtggat caccacacca
    14101 aagacatcca gcgtatcttc actactgatg gtaatgggcg agtacgcgct gttcatggga
    14161 ataagctgta ccgtcgggcg tagttgcaat tttttcaccg taaactcgcc gtcaacagca
    14221 gcgatgacaa tatcaccatg gctggcggta atagcgctat cgacaatcag taaatcaccg
    14281 tcactaattc caccatcaat catagaatca ccacttgctt tgacgaagta agtcgcgctg
    14341 ggatgctgga tcaacagttg attcagatcg atgcgctgtt caacgtaatc tgcggccggt
    14401 gaaggaaagc cacactgaac aagatcgcta aatagcggaa aagtcacaat ttcgcggaga
    14461 tccgcaggct tgataaacaa cataataatc tgcctgaagt tatactgttt ttatatacag
    14521 tagtctgttc ttgccagcag atcaatactg attcaggcta tcaatatttg tcgctgcata
    14581 gactgctgat ttttcgttct cttatcctgt gctcacgcgg ccttctggcg acgacgctca
    14641 tccagcagaa atgaaaaata tcacccggct aaaaaataga atagaagcat tcgccataat
    14701 gacattaaac attgtttgat atttatcata ttaatagaaa taaagacatt gacgtatctc
    14761 t
//
LOCUS       DBFIFM010000116        14749 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.116, whole genome shotgun sequence.
ACCESSION   DBFIFM010000116 DBFIFM010000000
VERSION     DBFIFM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.116"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(129..194)
                     /locus_tag="N7C74_003003"
                     /pseudo
     CDS             complement(129..194)
                     /locus_tag="N7C74_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444509.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(205..310)
                     /locus_tag="N7C74_003004"
                     /pseudo
     CDS             complement(205..310)
                     /locus_tag="N7C74_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
     gene            490..663
                     /locus_tag="N7C74_003005"
                     /pseudo
     CDS             490..663
                     /locus_tag="N7C74_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            774..1697
                     /gene="yicL"
                     /locus_tag="N7C74_003006"
     CDS             774..1697
                     /gene="yicL"
                     /locus_tag="N7C74_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HGU2432831.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFTAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYAGQGTNFIVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(1701..2519)
                     /gene="nlpA"
                     /locus_tag="N7C74_003007"
     CDS             complement(1701..2519)
                     /gene="nlpA"
                     /locus_tag="N7C74_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HGU2432832.1"
                     /translation="MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNNGELDANVFQHRPFLEQDNQTHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIEQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            2741..3034
                     /gene="yicS"
                     /locus_tag="N7C74_003008"
     CDS             2741..3034
                     /gene="yicS"
                     /locus_tag="N7C74_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HGU2432833.1"
                     /translation="MKPTMLLMITVFLIFPALSQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(3075..4265)
                     /gene="nepI"
                     /locus_tag="N7C74_003009"
     CDS             complement(3075..4265)
                     /gene="nepI"
                     /locus_tag="N7C74_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HGU2432834.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGCFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMTLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            4499..4852
                     /locus_tag="N7C74_003010"
     CDS             4499..4852
                     /locus_tag="N7C74_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU2432835.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADALGGGVFKKR
                     LHQNRERAIILAKGVSNWFYTFLYAKQDMSNINSQELAGFREIAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            4836..5159
                     /locus_tag="N7C74_003011"
     CDS             4836..5159
                     /locus_tag="N7C74_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2432836.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVNAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LEVLL"
     gene            complement(5275..5727)
                     /gene="yicN"
                     /locus_tag="N7C74_003012"
     CDS             complement(5275..5727)
                     /gene="yicN"
                     /locus_tag="N7C74_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HGU2432837.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(5780..7113)
                     /gene="adeQ"
                     /locus_tag="N7C74_003013"
                     /gene_synonym="yicO"
                     /pseudo
     CDS             complement(5780..7113)
                     /gene="adeQ"
                     /locus_tag="N7C74_003013"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
     gene            7288..9054
                     /gene="adeD"
                     /locus_tag="N7C74_003014"
     CDS             7288..9054
                     /gene="adeD"
                     /locus_tag="N7C74_003014"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HGU2432838.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYTDAPALQRIDAHGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESAKLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFERNDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(9100..10491)
                     /gene="uhpT"
                     /locus_tag="N7C74_003015"
     CDS             complement(9100..10491)
                     /gene="uhpT"
                     /locus_tag="N7C74_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HGU2432839.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(10629..11948)
                     /gene="uhpC"
                     /locus_tag="N7C74_003016"
     CDS             complement(10629..11948)
                     /gene="uhpC"
                     /locus_tag="N7C74_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HGU2432840.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(11958..13460)
                     /gene="uhpB"
                     /locus_tag="N7C74_003017"
     CDS             complement(11958..13460)
                     /gene="uhpB"
                     /locus_tag="N7C74_003017"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HGU2432841.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIEESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWLQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(13460..14050)
                     /gene="uhpA"
                     /locus_tag="N7C74_003018"
     CDS             complement(13460..14050)
                     /gene="uhpA"
                     /locus_tag="N7C74_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HGU2432842.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(14212..>14749)
                     /locus_tag="N7C74_003019"
     CDS             complement(14212..>14749)
                     /locus_tag="N7C74_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432843.1"
                     /translation="KKVVFTPGECDLVRDSLVQDGIKPEKIAKIISCLSSRTGIRNLF
                     YACSQAFHVANDNAALDVRRAATSVGDTIDVCPSFVALNGRNYHQHVDFSEYGFTQTL
                     CVLGAVVNNANDTFDVANYIYTTKGENIIHNDAEDDSPYVHSDESYRHKDDYWARDND
                     IRNLRRNFSGNITIATYK"
BASE COUNT         3985 a         3915 c         3507 g         3342 t
ORIGIN      
        1 aacctgaata accccgtact tacggagtta ttcaggttaa aaatatccat aacatcactt
       61 ctgattttta ttgcttacga cggatataat ttctctggtc agctactaat atcatcatgc
      121 tcacgcgttt aatcaaactc gtgtttctca aacggacatc gcgggacaat cttctgcttc
      181 tcccggcgca ttttttacca ccttcgctac ctgcattttg agcggaagat ccgctttttt
      241 ctggcccccg cacatatgca agtccagttt taaatttatc ctttatttca ttctgaaaca
      301 aactgctcat aattttctcc agtggaacct gcatctcagg gagacatcac tacagaatac
      361 ttccccttat gcactgattt aattttggtc atttagaata acggcctgta aagtctcaat
      421 gcaacagatg ggtaacgcta ttatgaggat cctcgtcagt aaattactgg caaggcactc
      481 cgataaataa gaataggttc cgcaacaacg ttatatgcaa atacctcacg cttcggctag
      541 attatcgccg gctctgttga cgaaattatg gttgaaatct ggagctacca cacgttgttc
      601 tggctggcga tagggatgtt gggtattgca atgatttgct tgctgtttat taaagatatt
      661 tagctcacca gtctgggcta agaaaaatcg gcgtggcgca aactgagcca tcctcgccat
      721 ttttgctatc atgcctgcat acataaacga caaaacagta tgcagaggga aaaatgggtt
      781 ccaccagaaa ggggatgctg aacgttctga ttgccgccgt gttgtgggga agttcagggg
      841 tctgcgcgca atacatcatg gagcaaagcc agatgtcgtc gcagtttttg actatgacgc
      901 gtttgatatt cgccggtttg attctgctga cgctatcatt tgttcatggt gataaaatct
      961 tttctattat taacaatcac aaagatgcca ttagcctact gattttttcc gtggttggcg
     1021 cgctaaccgt acagctcact tttttgctaa ccatcgaaaa atcgaacgca gccacggcaa
     1081 cggtgctgca attcctctca ccgactatta tcgtcgcctg gttctcactg gtgcgtaaat
     1141 cccgcccggg cattctggtt ttctgcgcta ttttgacatc gctgatcggg acttttttat
     1201 tggtgacaca cggtaatccg acgtcattat cgatctctcc tgccgcgtta ttctggggca
     1261 ttgcctcggc atttactgct gcattctata ccacctatcc ctcaacgctg attgcccgct
     1321 atggcacgtt accggtcgtc ggctggagta tgctcattgg cggtctgatc ctgctgccct
     1381 tttatgccgg acaagggaca aactttatcg ttaacggaag tttgattctg gcgttttttt
     1441 atttggtggt cattggtacg tccctgacat ttagtctgta cctgaaagga gcacaattaa
     1501 ttggcggtcc aaaagccagc attttaagct gtgctgaacc attaagtagc gcgctgctct
     1561 ctttgctgtt gctggggatc accttcacat tgccggactg gctgggaacg ctgctgattc
     1621 tgtcatcggt gattttgatt tcaatggatt cccgccgccg cgccagaaaa ataaatcgtc
     1681 cggcgcggca tgagtgagaa ttaccagcca ggcaccgcac caccgttaaa gatggtttct
     1741 gccgctttag cgacttcggg tgattgataa gattgcagaa attccttcac gttttctgca
     1801 ttcttattat cttcccgtgc caccagaata ttcacatacg gcgaattttt atcttcaata
     1861 aatacgctgt cgttaaccgg agaaagtcct gtttgctcga tgtaagtggt gctgataatc
     1921 gctacatcca ctttcgggtc gtccagtaca cgtggcaact gtgccccttc cagctccatt
     1981 atctgcaaat ggcgtggatt atcggttata tccaacgcgg taggtaataa cccttttccc
     2041 tctttcagag taattagctt ctctttttgc agcagtaaaa gcgcgcgacc aaggttagtc
     2101 gggtcgttgg ggatcgccac tgtcgcacct tcttttattt gcgccacggt tttgattttt
     2161 ttggaatagc ccgccatagg gaagacaaac gtatttccca ccgctaccag tttataacca
     2221 tgcgtctgat tatcttgttc aagaaagggt cgatgctgga agacgttcgc atcaagctcg
     2281 ccattatttg tcgcatcgtt cggtagcaac gaaccgctaa atcctaccag ttcaacatcg
     2341 aggccatatt tctcttttgc taccttttta gcgacttctg cgacatcttg ttctgcgcca
     2401 ttaataacgc caactttaat atgcttttca tcgctgctgc tctggtcgca acctgccagc
     2461 agaactcctg ccagtaataa tgcggccccc gcccgtagat gatgtgttgt cagtttcatt
     2521 tttttatcct tttattaatg attaccggac tatagcggta aggtcgctgc agtttaaaaa
     2581 acaaaacgct atcgataaga ataaaaagga ataaaagtgg aatataaagt ctggcacttc
     2641 ttactgacca cgcaaacacc tttcgtacag catgatgaga gcggcaaaag caaactacac
     2701 ttatgcttca ttcgatgcac atttgcagaa ggttgccgct atgaagccaa cgatgctact
     2761 catgattacc gtattcctga tttttccagc cctttctcag gccgagtcgc cattcagttc
     2821 tctacagtcg gcaaaagaga aaaccacggt attgcaagat ttacgtaaaa tttgcacgcc
     2881 acaggcgtca ttatcagatg aagcgtggga aaagttaatg ttgtcagatg aaaacaataa
     2941 acaacatata cgcgaggcca tcgtggcgat ggaacgtaat aatcagagca attattggga
     3001 agcgttgggt aaggtagaat gcccggatat gtaagaacgg caacaaccac tgaccaacgc
     3061 ttctttacct gatatcagga tttcttcatt ttcacctttg cagtcaccaa caatgcagtc
     3121 agcaacatca atgtgccgga caacatcagc ggcgaagtca gaccaatgtt atccaacgca
     3181 taaccgccga ttgccgcacc gcaggtattg gcaagctgaa taacggcaac ctgaatagat
     3241 ccagcctttt ctgcctgatc ggccaatgag cgagtgatcc acgttgacca accaacggga
     3301 attaaagcaa aggtcagccc ccagataatc gccacaccag tagcaacaat tttatcgctt
     3361 ccccacagcg tcagtaccag cgcgctcagg gcgagcacaa acggtgcgcc cgctaaggcc
     3421 agtttcaccg aacgtttgag aataaacgac gaaagcgatg tcccgacaaa gctggcgata
     3481 ccaaaactca gcagcaccag ggttaagcca tcaacaccga atcccgccag agtcatatac
     3541 actgggcgaa tataagtgaa aaaagcaaac tgtccggcaa aggacataaa gatggcgatc
     3601 atcccggcca tcactcccgg acgttgtaat aagcggaaag tattttgttt ctgatgcgaa
     3661 ggttcacctg gcagcgaagg cagtgatttg ataatccaga aaatacacag cacacccatc
     3721 gccgccgccg cattaaagac attgcgccaa ccgataagct cgcctaaaaa acagcccaac
     3781 ggcgcggcaa tcaccagcgc aatagaaacc gcgccgaaga tcaccgacag cgccttcggc
     3841 accgtacgcg gcggcaccag acgcatggtc agcgacgccg acatcgccca gaacccgccc
     3901 agcgccagcc ccagacaggc acgaccgatt aaaagcaaac tgaatgagtt agcaaaggaa
     3961 accagcaagc aggagagcgt cagcaaaacg gcaaacaaaa taacaacgta gcggcggtca
     4021 gtggcctgaa ttgtctgggt aataaacaaa ctggcaaaca ttgccacaaa ggcggtcacg
     4081 gtcaccgatt gcccggcaac cccttccgaa atgcctaaat cctgggccat tggcgtcaac
     4141 aaactgacag gcaaaaactc aacgataatc agacaggcga cgcaaaacgc caccgagaaa
     4201 acggctgacc agttcggtcg ggtgatggca tccgcgccgc ggttttcggc aataaattca
     4261 ctcatggtgt tacccgtggc atgtttcagg ggaaaagccg accactttaa cattgaaatg
     4321 tgtgacgcat ttaacgtttt tgcaactttt acggtgttgt gggaaaagct cgaggctgct
     4381 cgaagaaata aaaatcatag gacaaggtga tatgtatttt cgcgattgtg ttgggaacaa
     4441 gggataaagt attgcacttg gtgatatact ttaataacag gacctcaacg atatacgcgt
     4501 gtttaaaacc aaatggtttg caagggaagc caggtctcat gcaataacag atgaagagtt
     4561 gtgccgggcc attttagaaa cagaacaagg taaagctgat gcacttggcg gtggcgtttt
     4621 taaaaagcgc cttcaccaaa atcgtgagcg tgcaattatt ctggcgaaag gggtaagcaa
     4681 ttggttttac acgtttttat atgcaaaaca ggatatgtcg aacatcaata gtcaggaact
     4741 ggctgggttt cgtgaaattg caaagcatta tgccttcctg acgaaagccc aactaacagc
     4801 aatgattaac actaaagaac tgacagagat ctgctatgac tgcaaaaact aaatttaaaa
     4861 gtcctgcatt tgaggctatt cacagcgcag cggctggatt gtccagcgtc aatgctattt
     4921 ctgctgaaac gatgcgtact tttgacaaag cctgcctgac cagcgtgcaa gacttacaac
     4981 ctgttgagat aaaagcactg cgggaaaaat taaaagtgag tcagcccgtc ttcgctcact
     5041 atctcaatac cagcgtttca actgtacaaa aatgggaaag tggcgctaaa cgccccaatg
     5101 gcatttcatt aaaactgctc agcattgtgc aaaaacacgg gttggaggtg ctgctgtaaa
     5161 tagaacgtag ataatgcgtt cacctactgt cagttccgat accggatgcg gcgtgaacgc
     5221 cttatccacc ctacaggtca ggcgcggtct gtaggctgat aagacgaagc ggttttaatg
     5281 cagccagtga acgccctcgg caggctgaaa gaacgcgtta tagcgcatct gaaaagcatt
     5341 aatatcctgc atttcaggtt cggtttcgcg cagaaaaccc agcgccagcc tgacctgagc
     5401 atcagtaatg ggagcggcaa ggcgggcttt ttgtaaaatc cgatgccatt gcagcaggtt
     5461 ctgttcgcta ccatcgacaa tgacaatttg ccagatgagc aacacctgcg cggcgttttg
     5521 caggtgcgac tcatccggac gatgcaaata acgtaaaaat tcgtcaccgg ctgactttcc
     5581 catttgatcg accatcgact ccacgggtac gacatcgata ttcagccgat cgctgagacg
     5641 gcgaatcgct tttctggccc cggatgtcag cacccgaaaa ccaaccacaa acactaccgc
     5701 cagcgtggcg agcattatcc agatcatgag ttctcctgcg taatcagaac tcatgatatc
     5761 tggaaaccct cgccagttac taatccacca gaataatctt cagtgcaaac agagctgcga
     5821 ccaccaccac acacaggttc agatcgcgcc agcgcccggt gcacactttc atgatgcagt
     5881 acgacataaa gccaagtgca atcccttcgg tgatcgagaa agtaaagggc atcatcaccg
     5941 tggtaacaaa cgcaggcacc gattcggtaa aatcatccca gttaacgcgc gccagacttg
     6001 aggtcatcaa cacgccaaca aagattaacg ctccggcggt tgcgtaagga ggaaccatcg
     6061 ccaccagcgg tgagaagaac ataaccaaca ggaacataac gccgaccaca accgcagtca
     6121 gccccgtgcg accaccgact gccacaccag aagtactttc aatataggcg gtaacagacg
     6181 aggtgccgat aaacgcgccc gccaccgaac tacgctatca acatatagcg ccttattcat
     6241 attggggaat ttaccgttac tatctattaa gccagcttta tcagttacac caattaatgt
     6301 tcctgatgaa tcaaataggt tgatcagcat aaaggagaaa atgataccgg cgagttcaag
     6361 tgttaacgcg ccgctcaaat ctacttcacc aatgacgccg ctaatatcag gcggaatgga
     6421 atagacgccg ctaaaatgaa catcaccgaa aaataatcca cagcaagacg tcaccacaat
     6481 agaaacaagc accgcggcat gaaaatgacg tgatgacaac acggttataa taaaaaaccc
     6541 taaaatacct aacaacacgc cgtgagaact taaatcgcca atcatcacca gagtgtcttt
     6601 attggcgaca ataacgccag tattttttaa tcccattaag gcgataaata atccgattcc
     6661 gctggtgata ccaatacgta aacttaatgg aatgttggag atcatccagt accggatacg
     6721 aaatagcgtg agcaaaaata gcccaattgc gccccagaat atcgcgccca tcccggtctg
     6781 ccaggagata cccatcgccc ccacgaccac gaacgcaaag aaggcgttca gccccattgc
     6841 cggagccagc gccacgggta agttagcaaa tatccccatc gcaatactgc cgataccggc
     6901 aatcaaacag gtggtaacaa acaccacttt cgggtccatt tgtgccgcgc cgaggatttg
     6961 cgggttcaca aaaacgatgt acaccatggt taaaaaagtg gtcatccccg caatcaattc
     7021 tgtgcggacg gtggtcccat gctgaggtag tttaaataat cgcgaaagcg tccctgattc
     7081 attactcacg taatcggtat tgtcattatt catttttttg tccattggag gagatttaat
     7141 cccttgcttt tgtaaaacaa atgacaacaa cggttcagtg ataattatca catttcaatt
     7201 gcacattaat ggatattctt taataatctt gcgacatttc tttatgataa ataacaatca
     7261 aattaataaa atcaaaatga gaaaaatatg aataattcca ttaaccataa atttcatcac
     7321 attagccggg cggaatacca ggaattgtta gccgtttccc gtggcgacgc tgttgccgat
     7381 tatattattg ataatgtctc tattctcgac ctgattaatg gcggagaaat ttccggccca
     7441 attgtgatta aagggcgtta cattgccggt gttggcgcag aatacactga tgctccggct
     7501 ttgcagcgga ttgatgccca cggcgcaacg gcggtgccag ggtttattga tgctcacctg
     7561 catattgaat ccagcatgat gacgccggtc acctttgaaa ccgccaccct gcctcgcggc
     7621 ctgacaactg ttatttgcga tcctcatgaa atcgtcaacg tgatgggcga agccggtttc
     7681 gcctggtttg cccgctgtgc cgaacaggca aggcaaaacc agtacttaca ggtcagctct
     7741 tgcgtacccg ctctggaagg ctgcgatgtt aacggtgcca gttttaccct tgaacagatg
     7801 ctcgcctggc gggaccatcc gcaggttacc ggccttgcag aaatgatgga ctaccctggc
     7861 gtaattagcg ggcagaatgc gctgctcgat aaactggatg catttcgcca cctgacgctg
     7921 gacggtcact gcccgggttt gggtggtaaa gaacttaacg cctatattgc tgcgggtatt
     7981 gaaaactgcc acgaaagtta tcagctggaa gaaggacgcc ggaaattaca actcggcatg
     8041 tcgttgatga tccgcgaagg gtccgctgcc cgcaatctca acgcactggc accgttgatc
     8101 aacgaattta acagcccgca atgcatgctc tgtactgatg accgtaaccc gtgggagatc
     8161 gcccatgaag gacacatcga tgccttaatt cgccgcctga tcgaacaaca caatgtgccg
     8221 ctgcatgtgg catatcgcgt cgccagctgg tcgacggcgc gccactttgg tctgaatcac
     8281 ctcggcttac tggcacctgg taagcaggct gatatcgtcc tgttgagcga tgcgcgtaag
     8341 gtcacggtgc agcaggtact ggtgaaaggc gaaccgattg acgcgcaaac cttacaggcg
     8401 gaagagtcgg cgaaactggc acaatccgct ccgccatatg gcaacaccat tgcccgccag
     8461 ccagtttccg ccagcgactt tgccctgcaa tttacgcccg gaaaacgcta tcgggtcatt
     8521 gacgtcatcc ataacgaatt gattacgcac tcccactcca gcgtctacag cgaaaatggt
     8581 tttgagcgca atgatgtgtg ctttattgcc gtacttgagc gttacgggca acgtctggct
     8641 ccggcttgtg gtttgctcgg cggctttggt ctgaatgaag gcgcgctggc tgcgacggtc
     8701 agccatgaca gccataatat tgtggtgatc ggtcgcagtg ccgaagagat ggcgctagcg
     8761 gtcaatcagg tgattcagga tggcggcggg ttgtgcgtgg tacgtaacgg ccaggtccaa
     8821 agccatctgc cgttacccat tgccgggctg atgagcaccg acacggcgca gtcgctggcg
     8881 gagcaaattg acgccttgaa agccgccgcc cgtgaatgcg gtccgttacc cgatgagccg
     8941 tttattcaga tggcgtttct ttccctgcca gtgatccccg cgctgaaact aaccagccag
     9001 gggctgtttg atggcgagaa gtttgccttc accacgctgg aagtcactga ataataaaaa
     9061 aagcccggcg tcatgccggg caaaagtcac cagttacgtt tatgccactg tcaactgctg
     9121 aatttttttc tcgcggcgga ttttgcgttc ttccatcacc gccactatcg ccatcaggca
     9181 gatacaacca atcgcggcga tatccagcgc agcgaaggtg cctgcccagc cggtaaggcc
     9241 gaatactggc gtcccatcgg caatcattcc cagacctaac ttggcaaagc tgtcaccaat
     9301 caggtaagcg aaggtgcctt taataccatc ggcagcgcca atcgcttttt taggtacaaa
     9361 gccaacagca gccacaccaa tcaacaattg cgggccaaag accaggaaac ccaacgcaaa
     9421 gagagaagcc agatagatat attcgttgct ggcatgttgg tacacaccga gcgtggcgat
     9481 aatcagcgcc agcgcgatgc aggccaccag gccacggcga ccgttcgcta ggtcagagag
     9541 ccagccccac agcagcgtac cgaccagcgc accagcttca aacagcgtaa agccctgaat
     9601 cgccaccgct ttagagagtt tcagttcctg gaacgcgtac acagttgacc actggtcgat
     9661 accaatacgc accacataga ggaaaatgtt ggcgaagcac agcagccaga tcactttgtt
     9721 tttcagcaca tactcaacaa agatctgcca cttggtcata tcggtagatt ctgtctcttt
     9781 gtcctcttcg ctgatctcct cgccgaacag ctcttcagct ttgccgaggc cataagattc
     9841 cggggagtcg ctgccgtaac gcaggccgat aaaaccgaca atcagcgcga taatcgacgg
     9901 gaagataaac atgccgatga catggccatc gaacaggtaa tttgcgccga acagtgccac
     9961 accagctgcg cctgcaccgc caaggttgtg agaaatgttc cagaaaccga ggaacgtccc
    10021 gcgtttacga cgcggcgtcc atttggtgat ggtggagtaa ctgcacgaac ccccggtact
    10081 ctggaaaaag ccacttaagg cgtagaaggc aatcatcagg aacaggctaa ccgagccgct
    10141 gcccatactg gcgctaaagc ccagcataca aatggcagac aggatcagca tgaacggcag
    10201 gaattgtttg gtgtttttgc cgtcggcgta gtaggaaacc agcgttttac ccacgccata
    10261 agtgatggag aaacccaggc cgatcatccc cagctgcgtc atgctcaacc cgtaggtcga
    10321 aatcatatcg ttctgcgcga tgttaaagtt cttgcgaatc aggtacatcg tcaggtagcc
    10381 gataaagacc accaggtagg attgcatgaa cggtttgaac cacattttgc gccgcacttc
    10441 gagcggaagg tccagggtcg gcttgcggac ctggtttaag aaagccagca tgggttactc
    10501 ctgaaatgaa tacctgcgtg aggcatgcat tgtaaaaatc agcagcaaat caggcctgag
    10561 atagcgtcca ggcaaaacct ggaaaatttc ttagttttgc cggatatgaa gtgaaaaggt
    10621 gagatgcatc acgcttcgcg cggtgtctgg gcgttcaaaa agggcagtaa cagcagtgcg
    10681 gaaatcccgg cggcaatggc gataaccaca aaaaatccgc tccagtgcca ggtatcgagc
    10741 actttcgcca gcggccaacc agcaagcgac gcccccagat aagcaaacaa gccgacaaac
    10801 cccgtcgccg cccctgccgc ctctttgtgg gaacattctg ccgccgccat gccgattaac
    10861 atctgcgggc caaagacaaa aaaaccaatg gtgaagaagc aggttgcctg catcacgtag
    10921 ctggcaaatg gcatcagcca cagcgagccg actgaaagca aaattccggc agcgaaaatc
    10981 aaattcatcg gcccgcggtt gccgttaaac agtttgtccg agccccaacc cgctaccagc
    11041 gcaccgataa atccgcccag ttcaaacatc gtcactgccg tattcgccgt gaccagatcg
    11101 acgcccagcg tctcggacat atacaaattg ccccagtcgt tgatcgccgc ccgaaccaca
    11161 tagaccagca catagcaaaa cgaaagcagc cagatatacg gattcaacaa cacatacttg
    11221 gtgaggatct ctttacgcgt cagccctgcc ccttcttgtt gttgagcaat ctccagcgcg
    11281 tcgtgtcgcc attcaccgac cgctggtaaa cctaacgcct gcgggcgatc gcgcagccgc
    11341 cagcagagaa aaatccccac gacgatcgcc atacaaccag caatcatcat cccggcacgc
    11401 cagccgtaat gcagcgcagc tgctgccatc acaatgggaa tgagcgcacc gccaacgtta
    11461 tgcgccgtgt tccataatgc ccaccaacca ccgcgttcgg tacgagaata ccaggccgtt
    11521 aacagacgcg cacacaccgg tgaaccccag ccctggaaaa aggcgttcag cacccagagc
    11581 acggcaaacg cccatagcga cgtcgagaag ccaaacagaa tgttgatgat gcccgtggcg
    11641 ataagcccta tccccataaa ataacgggca tttgagcggt cgctgacaat gccggagaca
    11701 aacttcgaca cgccgtaggt aatgtaaaac agggtcgcta acaggccgat atcgctacgg
    11761 ctgagcacgc cattagcaag gatttctggt acggcggcgt taaaactttt acgcgtgaag
    11821 taaaacagcg cgtaacccag ccagatggtc agcagaatat gccgacgcca gtagcgataa
    11881 cgggcatcaa tttcatgttt atcagtcatt aatggcgcat cggcaggcgc tttcagaaac
    11941 ggcaacatca tcaaacctta gacatagcgt tgaggtagag aaacgctgac acgcgtgccg
    12001 tgcagacagg aaatggtcaa ggtgccgccc agcgccgtta cgcgctcgcg cattccggtg
    12061 aggccaaaac cttgttgccc ggaacccggc ggtaaaccgc tgccatcgtc ttcaatcacc
    12121 agcatcaacc gttcatcctg cagccagcct tgcagggtga ccgcgctggc atcagcatgt
    12181 ttcacaatgt tattcagccc ttcctggcag acacgaaaca gcgtcacgcg ctggttttcg
    12241 cttaacgctg actcttcgat tcgccattcg agatggctga caatgccgcg cccttccagc
    12301 tccatttccc gcatcagtga gcggatggcc tgctctaggg tgagatcatc caactggcgc
    12361 ggacgtaacc gccctaacaa acggcgcacc gcgtcgtaca cgcccagcga cagttgttcg
    12421 atgagctgcc cgctctgctt cacactggcg ttatctgccg ccagtcgctg gacaatgccc
    12481 gcttgagtac gaatggcagt gatggtctgg ccgatatcat catgcagctc acgcgccaca
    12541 tcacggcgca cgctctcttc agtttctaac aaacgttcag cgagatgctg attgcgcgcc
    12601 agttcctttt gcagcgactg gttaagttca cgcaaccgct ggatgccagc gcccagtaac
    12661 aaccctgtca gactttgcac cagcagcgag agcaataaat ccacaggatg atcgcgccag
    12721 gtttgactgg cgatcagcgc gatggcgttc atcaatgtcg ctatcagcgc cccttgccag
    12781 ccgtagtgcc aggccagcgc gataatcggc agcgccaggc agaatggcgt aaagcgcgac
    12841 agttcgtccg gcaagcccaa ctggagccag agactgataa caaacagcag caagtaccag
    12901 accagatgcc gcccgcgcca gttgatcggt tgagaaacta acgacggccc gagcggcagc
    12961 caggtgttat tggcgagata atgccagaac accagacata tcggggccag cgtcaggccg
    13021 ccagttaaag ttaacagcag cgcattccac gactctttgc cgtgccaaag ccagggcagc
    13081 gactgcaaca acgccgccgc cgttaacgcc gcaccctgta acagcaaggt gcgccagtca
    13141 cgttgatggc gatagcgcga gatcagcgcc acgggcagta acgtcagtaa actaccgatc
    13201 atcaataagg agaaatgggt taaaccgacc gcctgcgtta gccagtaaat cagcagccac
    13261 tccgctccca gcagcaccgg ccagtatccg cgcgggcatt gcagcattag ccctagccgc
    13321 agaccaaacg gaaataacag caccgccata tcagggcgct caaccaggtg caggctgata
    13381 ctccacaggc aaaaccatgc ggcagagaag ataaaaaagc aggcaataac ggtaattaag
    13441 cgggagaaca acgtcttcat caccagccat caaacatacg gcgcgccagt tcaacgtcgt
    13501 tactgacgcc cagtttttcc atcagattgg cgcgatggac gtgtaccgtt ttcggtgaca
    13561 agcccagttc ggcggcaatc tctttcaccg ccattccttg cgccagtttt tccgccacct
    13621 gccgttcacg tttggttagt gggtcctggc gaccggatgc cagtttaatg gcaatatccg
    13681 gcgtcagata acagccgcct gtggcaaccg tatgcaccgc agcaatcagt tcgtcagggc
    13741 tacaacgctt ggagagaaag ccacgcgccc ccgcgttaag cgcctgctca accagcgccg
    13801 gactgtcatg aacggagagc ataattgtcg ccataccttt cggcagctgg cttagcagct
    13861 ccagaccgga gatatcgggc atggagatat cgcaaataca cacctgcaca ccgcgccccg
    13921 gcagccccgc cagcgcctcg cgccccgaac caaactcggc aactacttgc aaatcaggtt
    13981 ccagccccag caactgcgca aagccggagc ggacgatgag gtgatcgtct ataagggcaa
    14041 cggtgatcat gattgggtcc tggcggataa aaaacgcgca taccttaacg ataagcgcga
    14101 tgttgttcaa gttggatatg gggtatgtat tggcttaatg atgttgagct tatcgtcaaa
    14161 acgcttacct gagaggtaag cgtttggtgc gtgttattga gaaaaagcga attatttgta
    14221 ggttgcaatc gtaatattgc ctgagaaatt tctccttaga ttgcggatat cattatccct
    14281 tgcccaataa tcatctttgt gtctataact ctcatcggaa tgtacatagg gagaatcatc
    14341 ttcagcgtcg ttatggataa tattttcgcc tttagtggta tagatataat tagcaacatc
    14401 aaaggtgtca ttcgcgttat ttacgactgc gcctaagacg caaagggttt gagtgaatcc
    14461 gtattctgag aaatcgacat gctgatggta atttctacca ttcaaagcaa caaaactcgg
    14521 acaaacatct attgtatcgc caacagacgt agcggcgcga cgcacatcaa gtgcagcgtt
    14581 atcgttagca acatgaaatg cttgtgaaca ggcataaaac aaatttctaa tcccggtacg
    14641 actggataaa caagaaataa tttttgctat tttttctggc tttataccat cctgtactaa
    14701 agaatctctc accagatcac attcaccagg tgtgaaaaca actttcttg
//
LOCUS       DBFIFM010000117        14513 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.117, whole genome shotgun sequence.
ACCESSION   DBFIFM010000117 DBFIFM010000000
VERSION     DBFIFM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.117"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..340)
                     /gene="acpS"
                     /locus_tag="N7C74_003020"
     CDS             complement(<1..340)
                     /gene="acpS"
                     /locus_tag="N7C74_003020"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HGU2432844.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLAD"
     gene            complement(340..1071)
                     /gene="pdxJ"
                     /locus_tag="N7C74_003021"
     CDS             complement(340..1071)
                     /gene="pdxJ"
                     /locus_tag="N7C74_003021"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HGU2432845.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1083..1811)
                     /gene="recO"
                     /locus_tag="N7C74_003022"
     CDS             complement(1083..1811)
                     /gene="recO"
                     /locus_tag="N7C74_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HGU2432846.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(1823..2728)
                     /gene="era"
                     /locus_tag="N7C74_003023"
     CDS             complement(1823..2728)
                     /gene="era"
                     /locus_tag="N7C74_003023"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HGU2432847.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVATIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(2725..3405)
                     /gene="rnc"
                     /locus_tag="N7C74_003024"
     CDS             complement(2725..3405)
                     /gene="rnc"
                     /locus_tag="N7C74_003024"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HGU2432848.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(3678..4652)
                     /gene="lepB"
                     /locus_tag="N7C74_003025"
     CDS             complement(3678..4652)
                     /gene="lepB"
                     /locus_tag="N7C74_003025"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HGU2432849.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(4668..6467)
                     /gene="lepA"
                     /locus_tag="N7C74_003026"
     CDS             complement(4668..6467)
                     /gene="lepA"
                     /locus_tag="N7C74_003026"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HGU2432850.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(6665..7144)
                     /gene="rseC"
                     /locus_tag="N7C74_003027"
     CDS             complement(6665..7144)
                     /gene="rseC"
                     /locus_tag="N7C74_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HGU2432851.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7141..8097)
                     /gene="rseB"
                     /locus_tag="N7C74_003028"
     CDS             complement(7141..8097)
                     /gene="rseB"
                     /locus_tag="N7C74_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HGU2432852.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(8097..8735)
                     /gene="rseA"
                     /locus_tag="N7C74_003029"
     CDS             complement(8097..8735)
                     /gene="rseA"
                     /locus_tag="N7C74_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HGU2432853.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHEWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(8768..9343)
                     /gene="rpoE"
                     /locus_tag="N7C74_003030"
     CDS             complement(8768..9343)
                     /gene="rpoE"
                     /locus_tag="N7C74_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HGU2432854.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(9340..9495)
                     /gene="rseD"
                     /locus_tag="N7C74_003031"
     CDS             complement(9340..9495)
                     /gene="rseD"
                     /locus_tag="N7C74_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HGU2432855.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            9751..11373
                     /gene="nadB"
                     /locus_tag="N7C74_003032"
     CDS             9751..11373
                     /gene="nadB"
                     /locus_tag="N7C74_003032"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HGU2432856.1"
                     /translation="MNTLLEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIISDKIGLPGTRRVVGAWVWNRNKEKVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDVRGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHDISTLPPWDESRVENPDERVIIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(11358..12095)
                     /gene="trmN"
                     /locus_tag="N7C74_003033"
     CDS             complement(11358..12095)
                     /gene="trmN"
                     /locus_tag="N7C74_003033"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HGU2432857.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKAGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAATQAQENVAQSPWLERINVHT
                     ADIQQWVTQQTARFDLIISNPPYYEQGVECATPQREQARYTTSFDHQTLLTCAAECIT
                     EEGFFCVVLPEQIGNSFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12227..13561
                     /gene="srmB"
                     /locus_tag="N7C74_003034"
     CDS             12227..13561
                     /gene="srmB"
                     /locus_tag="N7C74_003034"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HGU2432858.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(13594..14475)
                     /gene="yfiE"
                     /locus_tag="N7C74_003035"
     CDS             complement(13594..14475)
                     /gene="yfiE"
                     /locus_tag="N7C74_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2432859.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEHSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEDCFLPG"
BASE COUNT         3480 a         3945 c         3615 g         3473 t
ORIGIN      
        1 catcggccag cgtcacatgc atatttgcaa cgcccagctt ttccgccagt tttaatgcct
       61 cgccccacag tcgcagccgt ggcttaccaa gctcatcgtt aaatacttca aactgattaa
      121 acgccagacc attgcggatc ccggtgccaa acgcttttgc tgcggcttct tttacggcaa
      181 agcgtttcgc cagaaaacgc accggctggt ggtgcgtttt ccaaatttcc cattcgttat
      241 cgcttaatac gcggcgtgcc aggcgctcac cggatcgggc gatcactgct tctatgcgag
      301 cgatctctac gatatccgtg cctaaaccta atattgccat tagccacgcg cttccagcat
      361 caggcgcttc atttctgcca ccgcatcttt cagtccggtc atcactgcac gaccaataat
      421 ggcatgaccg atattcagtt cgtgcatctc agggatggcg gcaatggctt ttacgttgtg
      481 ataggtcaga ccgtgtccgg cgttaacttt caaaccgaga ctagcggcaa aggtcgcggc
      541 tttggcgata cgcgccagct cttgcgcctg ttcggcgtca gttttggcat cagcatagca
      601 accggtgtgg atctcgataa acggtgcgcc aacctctgcc gcagctttga tctgctcttc
      661 atcggcgtcg ataaacagag aaacctgaat cccggcatcc gccagacgtt tgcaggcatc
      721 gcgcattttg tcacgctgcc ctgcaacgtc caggccgcct tcggttgtta cttcctgacg
      781 cttttctggt accaggcagc aaaaatgcgg cttcgtctca accgcgatcg ccagcatctc
      841 ttcggtcacc gccatctcca gattcatgcg ggtatccagc gtctgacgca ggatgcgcac
      901 atcgcggtca gtaatgtggc ggcgatcttc acgtaaatgc acggtaatgc cgtccgcccc
      961 cgcctgctcg gcaataaacg cggcctgcac cggatccggg taagcggtac cgcgcgcgtt
     1021 gcgcagcgta gcgatatggt caatgttgac gcctaacagt aattcagcca tgacaatcct
     1081 catcattcat aatgtgtttt caccgttcgc ttaggcataa attgccggaa cagttccctg
     1141 ctctttaaag gtttaccgcc aagatacggc ttaagcgcca tgcgggtaaa gcgtttcgcg
     1201 gcgcgcagtg tgtctgcgtc aggaaattcc cgtgcgttta acgcttttaa ctgccttccg
     1261 gtgaacgttt tattgtcgat aacgacgctt gcgataaacc ctttttcttc gcgataacga
     1321 tacgtcatgg tgtcatctac cggctcgccg ctacccgcac aatgggtaaa attgacgcca
     1381 taacccagat gcccgagcag tgccagttca aagcggcgca gcgcgggttc tggcgtacca
     1441 gtgacccctg caagagactg aatgcagtgc aagtaatcga aaaagagttc agagaagcgc
     1501 gtctcgtatt ccagtacgcg ggagagaagt tcgttgatgt acagaccgct gtaaagcgtg
     1561 ataccgctta atggcagcgc cagcgagaca gcttcagcac tgcgcagcgt tttgacttcg
     1621 ccacgcccgc caaaacgtag caagagaggg gtgaaaggct gtaatgcacc tttcagggta
     1681 gagcgtttag agcgtgcgcc tttggcaacc agacgcacgc gacccgattc ctccgtgaag
     1741 acgtccagca tcaggctggt ttcgctccac gggcgactat gcaggacaaa tgcgcgctgc
     1801 cagccttcca tcggagttac tcttaaagat cgtcaacgta accgagactg cgcagtgcgc
     1861 gttcgtcgtc ggcccaaccg gatttcactt ttacccacag ctcaaggtga acaggcgctt
     1921 caaacatttc ctgcatgtct ttacgcgctt caatcccgat ggttttgatc ttggcccctt
     1981 tgttgccaat gaccatcttc ttctgccctt cacgctcaac gagaatcaaa ccgttgatgt
     2041 cataaccacc gcgttcgtta gagacgaaac gttcgatctc cacggtcacg gagtacggca
     2101 gttcagcgcc gaggaaacgc atcagttttt cgcggatgat ttcagacgcc ataaaacgct
     2161 gtgagcgatc ggtgatgtaa tcttccggga agtgatgagt cgcttcaggt agatgcttac
     2221 gcacgattgc cgcaatagtg gcaacattca gcccggtttc ggcagagatt ggcacgatat
     2281 cgaggaagtt catctggctt gccaggaact gcaggtgcgg cagcagatcg gctttctcct
     2341 gcacgttgtc cactttgttc accgcgagga ttaccggcgc tttgccttcg cgcagtttgt
     2401 tgagcaccat ttcgtcgtcc ggcgtccagc gggtgccttc aacgacaaaa atcaccagct
     2461 caacatcgcc aatagagctg ctcgccgctt tgttcatcag gcggttaata gcgcgttttt
     2521 cttccatatg caggcccggt gtatcgacgt agatcgcctg atacgcgcct tcagtatgga
     2581 tccccacaat gcggtgacga gttgtctgcg ccttgcggga ggtgatggag attttctgcc
     2641 ccagcagttt gttcaacaat gtggatttgc caacattcgg acgtccgacg atggcaataa
     2701 atccgcagta acttttatcg atgctcattc cagctccagt tttttcaacg cctgttcggc
     2761 ggcagcctgc tcagccttac gacggcttga acctgtgcca accaccggtt cactcaggcc
     2821 gctgacctgg cagtggattg taaattcctg atcgtgcgct tcgccacgga cctgaactac
     2881 cagataagtc ggcagcggca gatgacgacc ctgcaaatat tcttgcaagc gcgttttcgg
     2941 atctttttgt ttatcgcctg ggctaatttc gtccagacga gtttgatacc agttgaggat
     3001 taatttctcg acggtttgaa tatcactgtc gaggaatacg ccaccaatta atgcttcgac
     3061 ggtgtcggcg agaattgact cacgacgaaa tccaccgctt ttaagttcac ctggtcctaa
     3121 acgtaagcac tcgcctaact caaattcgcg cgccagttcc gccagcgtat tgccacggac
     3181 cagcgtggcg cgcatccggc tcatatcgcc ttcatccaca cgagggaaac ggtgataaag
     3241 cgcattggcg ataacgtagc tcagaataga gtcgcctaaa aattctaaac gctcgttatg
     3301 tttgctgctg gcactacgat gagttaatgc ctgctgcaac agttcctgat gattaaaagt
     3361 gtagcccagc ttccgttgaa gccgattaat tacgatgggg ttcatgcgat accaataaat
     3421 aaatgcgtca acaattcagc acacgaaaca gacctgatat acatggctct gctaactgct
     3481 tcgctgcagt ttcagtttag cggtatatgg ggaccaacgc tgtttcgtgt gccgtggcaa
     3541 cctggaggtg ccaaccttaa acttcggggg aatattctat acacaacgac gggggatgtc
     3601 gttagccacg ggagatttat ctcataaata attcacgttg tcgccataac ggcgacaacg
     3661 tgaacgaaga tgagctatta atggatgccg ccaatgcgac ttaagcgcac acctgtcggc
     3721 cattcgcctt cttgcttatc gaagctcatc cagatagccg ttgcccgacc gaccagattc
     3781 gcttcaggca caaagcccca gtaacggctg tccgcgctgt tgtcgcggtt gtcgcccatc
     3841 atgaagtatt gtcccggagg aacaatccag gttgccagtt gttgccctgg ctgctggtaa
     3901 tacatcccca cctgatcctg cgcaatcggc actgtcagaa tgcggtgcgt cacatcaccc
     3961 agtgtctctt tacgctcgga aagacgaatt ccattttctt tggtttcgtt tttcggcact
     4021 tcaaagaatc cgctggtcgc ttccccacca ttacggcgtg agaaggtctg aacgaaatcg
     4081 ctcggttcca cgtttgagta ggtgaccggc agcgcgtttt cacacgcctg gccggaactg
     4141 catcccggtt gaatcgtcag ctcttttgag accggatcgt aagtgacttt atcgcccggt
     4201 aaacccaccg cgcgcttgat ataatcaagc tttggatctt ccggatattt aaagaccacg
     4261 atatcgccgc gtttcggatg accggtttcg atcagcgttt tctggtagat aggatcttta
     4321 atgccataag caaacttctc taccagaata aaatcaccga ttaacagagt cggcatcatc
     4381 gaacctgacg gaatctggaa cggttcataa ataaacgaac gcacaatcaa tacgatagcc
     4441 agtaccggaa aaacagaagc accggtttcc agccagccag gcttcggcgc aacctttttc
     4501 aacgttgctt tatccagtga gtcaccggca gccgcctgcg ccgctgcctg acgttcccgc
     4561 cgtttaggtg cgaaaaagaa tttatccacg caccataaaa tgcccgtcac cagtgtggca
     4621 atcaccagaa tcagggcaaa catattcgcc atgccaactc ctaagggtta tttgttgtct
     4681 ttgccgacgt gcagaatggc gaggaacgct tcctgcggca gctcgacgtt accgatctgc
     4741 ttcatgcgtt tcttaccttc tttctgcttc tgcagcagct ttttcttacg gctgatatcg
     4801 ccgccataac atttagccag tacgttttta cgcagctgtt tcacggtgga tcgcgcaata
     4861 atgtgcgtac caatcgctgc ctgaatggcg atatcaaact gctggcgtgg gatcagatct
     4921 ttcatcttct ccaccaactc gcgaccgcgg ttttgcgaat tatcacggtg ggtgatcaac
     4981 gccagcgcat caacacgttc accgttgatt aatacgtcta cacgtaccat gtcggacgcc
     5041 tggaagcgtt tgaagttata atccagagac gcataaccac gcgaggtaga tttcaggcga
     5101 tcgaagaagt cgagcaccac ttccgccatc gggatctcgt acgtcagcgc cacctgatta
     5161 ccgtggtaaa ccatattggt ctgcacgccg cgtttttcta cgcacagcgt aataacgttg
     5221 ccgagatatg cctgcggcag cagcatgtga cactctgcaa tcggctcgcg cagttcgtaa
     5281 atgttattta ccgcaggcag cttggatggg ctgtcgacgt agataacttc ccttgacgtg
     5341 gtttcaactt catacactac ggtcggcgca gtggtgatca gatccagatc gtattcacgt
     5401 tccagacgct cctggatgat ctccatgtgc agcaggccaa ggaagccgca gcggaaacca
     5461 aagcccagcg cgctggagct ttccggctca tagaacagtg aggcatcgtt caggctgagt
     5521 ttacccagcg cgtcacggaa ggcttcatag tcgtcggaac ttaccgggaa cagaccggcg
     5581 tatacctgcg gtttgacttt cttaaagcca ggcagcgcct tttctgccgg attacgcgcc
     5641 agcgttaagg tatcgccgac tggagcgccg tggatatctt taatcgcaca tacgagccag
     5701 cctacttcgc cacatttcag ttcagtgcgg tcaacctgtt tcggcgtgaa gatgcccaga
     5761 cggtcggcgt tataggtctg cccggtactc atgactttca ctttgtcgcc cttacgcagg
     5821 gtgccgtttt taatacggat aagtgaaaca acgcccaggt agttgtcgaa ccatgagtcg
     5881 ataattagtg cctgcaacgg gccttccgga tcgccttccg gcggcggaat gtcgcgcacc
     5941 agacgttcga gaacgtcctg cacgccaacg ccggttttcg ctgaacagcg caccgcgtca
     6001 gtggcgtcga tgccgacgat atcttcaatt tcttccgcca cgcgttcagg atcggctgcc
     6061 ggcaggtcaa tcttgttcag taccggcacc acttcgagat ccatttccat ggcggtgtag
     6121 cagtttgcca gggtttgcgc ttccacgccc tgcccggcgt cgaccaccag caatgcacct
     6181 tcacaggcag ccagcgaacg ggaaacttca taggagaagt ctacgtggcc cggggtgtcg
     6241 ataaagttaa gctgataggt ttcgccgtca gacgctttgt agtccagcgt cacgctttgc
     6301 gctttgatgg taatgccacg ctcacgctca agatccatgg aatcgagaac ctgcgcctcc
     6361 atttcacggt cagacaggcc accgcagatc tggataatac ggtcagacag cgtcgattta
     6421 ccgtggtcaa tgtgagctat gatcgaaaag ttacgtatat tcttcataaa gtatgattat
     6481 tgtgccttaa tgcccggtta accaggcttt tagaagtcgc tgttctgagc ttaacgtctg
     6541 tattaataga aacgccgcat tctacactac aacattgagg cgaggaaatg ttcataccgt
     6601 atggattgtg gtatctggaa acgtcctcgc atttgttatg caaaatgcaa caaagccagt
     6661 gaaatcactg gctcgcgtct tccgaagatg tttcaaatcg cacaaggcca ggcggcaatg
     6721 ccacgcttaa gatgatcggt tgccattctg cccgggcagc aaacttacgc gagtagccac
     6781 gcgcaatcag gaacccgcca ataccgccga ggaccgcacc acataatgct gcgacgtcag
     6841 aagcaaagag tagctgaaat agcgaagcga tgaggaataa tcccaccagc ggcgacatat
     6901 aaaccagtaa tgcggagcta agcaggctgc cttcggcgat ccccaattcc actttttgcc
     6961 ccggcactaa cggctcatca cagggtacga caatggtatg cgtggtttgc gggccaagtt
     7021 tattcaacac gcggctaccg caaccggcgc gtgaagcgca gctgctgcat gaggctttaa
     7081 catcacaact gaccagcgcc tgcccgtttt gccaggagac gacggtagcc cactctttga
     7141 tcattgcgct gccccgaact taatattctc ggcaatgcgt ttcgccgttt gcggcggcag
     7201 ttcaccgaca atggtgattt cggcgttatc acgtacgctt gtactgacgg ttctgcgtcc
     7261 ggtgcgcaac atctgatcgg tgctcgatgg cgtagcgcgg ttaacgttta ccgagaagct
     7321 gaataatccg tcggaataga gacgtgattc gataggcatg ttgtccatcg tcggtagcgg
     7381 acgtcgacta ctggaaactt cgctaaaacc ctgtggcaac caggttggcg tccagctgaa
     7441 tttagctttt tcacctacag gaacagaaag caacggcggc aaatttgcct tcgccagcgt
     7501 ctgcatactg ctgctgatat cctgattgac gttaaaagca atcacgcgaa attgttccag
     7561 cgtttcacca tcgcgatcaa gaagatcaac ccgcatcggt aatttcgatt cggtgtccat
     7621 ccacacgatg tagctgtagc gtgtaccatc tcgggcaacc acgcgaatga cttcgcaaag
     7681 acgatcagca atacgcgtgc gcccgacgga gataaagtcg tagtaaggag aaaggcgttt
     7741 gaaatcggta tagatgagcg atggcagaga atcaacaatg taatcgccat taagcgtgaa
     7801 cggttcaagt cccggttcaa aatagctgat ttcattgccg cgctgtacca cttcccggcg
     7861 cgggccatcc atttgcaaca attgtgcaag aggacggtta tcgaggcgtg catgtcgata
     7921 acgcagagac tcaacaccct gtttattgat gctgatgaat gacagctcgt aattcagtga
     7981 ctgactggcc aggttcatct gctgtaataa cgccccggac gcgggagtgg ccgaggcgtt
     8041 agcagagaat aacaggctac ctgtcactaa tgacatggca aaccaaagtt gcttcattac
     8101 tgcgattgcg ttcctaaagt ttgaattcct ggcacctgta cagcggcttg ctgggtttgc
     8161 gcctgctcaa actgaagctg ttcagagtgg agtcggcgtt gcagttcgta atcctgcaac
     8221 attgcattaa tgcgacgacg ctgctgctgt tgaccattat ttgcggtcgc ttcagaaggt
     8281 actcccaggc taaccgggct ggctttaccc atcatcggca gtgtattaaa taccggcgtt
     8341 tcgggctgct gggacgtttc agattgtcca ttatagtgct ggacgccaac gataactgca
     8401 agcgatacgc atgcggctac gcccatttgg gtaagctgtg ccgcccacgg acgtactttc
     8461 tgccagaatg gcattttctg ccattcatgc ggcgcaggct gggcttccgg gatcaatgtc
     8521 gccggttgac gtactggctc ttcttcaatg gcggccatca cgcgtgaaga gatatcgaaa
     8581 tggagcacct cgggagtatc accccgcatt gagtcacgga ttaagtgata gctttcccag
     8641 gttttctgca tttctgggtt atgagccagt tcgttaagca gctcactatc cagcgtttcg
     8701 ccatccatta aagcggaaag ttgttctttc tgcatgccta atacccttat ccagtatccc
     8761 gctatcgtca acgcctgata agcggttgaa ctttgttatc aatagcttcc ctcgctcgga
     8821 agatacgtga acgcaccgta cctaccggac aatccatgat agcggctatc tcttcatagc
     8881 tcaggccatc cagctcccgc aaggttattg ccatgcgtaa atcttccggg agggactcaa
     8941 tagttcggaa aactatctgt ctcagttctt ctgacaacat taagttctca gggttcgaaa
     9001 tttctttcaa cgcgccgcca ctttcgaagt tttcagcttc aatggcatcc acatcactgg
     9061 aaggtggacg acgcccctga gcaaccaggt aatttttcgc tgtatttaca gcaatccgat
     9121 acagccatgt ataaaaagcg ctatctcccc ggaacgaatc cagcgcacga taggctttaa
     9181 taaaagcttc ttgtaccaca tcgggaacat cacccgacgg cacatagcgg gaaaccagac
     9241 tcgccacttt atgctgatag cgcactacca gtaagttaaa ggctttctga tctcccttct
     9301 ggacccgttc aaccaggacc tggtccgtta actgctcgct catccgaggt aaagtctccc
     9361 caaaccaaat ttccacgcgc tatcgaaacg ccactccatt agctgcaatt tgagcaagca
     9421 aagggttaga gtgtctcgtt tttgtaaagt tccgtaacgc atctgttttt gtttgtcatg
     9481 ctgtagacgg atcattatct atcattataa gtctacagaa tctgaacatc gcattatctg
     9541 tgtagaaatg cccatttaac tgcctgaaga gtaacccaac ggccttttta tttcaccacc
     9601 taatcctcca ccagccagta acttctcttt ttctcgccgc cctgcgtcag cgtgtttagt
     9661 cactgtaaca aatattaaaa tagcaggtgt ttattcgcac aacatgatgc tatgctgacc
     9721 aaaccgtgtt tagtaaatta aacaaagaaa atgaatactc tccttgaaca ttcatgtgac
     9781 gtgttgatta tcggtagcgg cgcagccggg ctttcactgg cgctacgcct ggctgaccag
     9841 catcaggtca tcgttctaag taaaggcccg gtaacggaag gttcaacatt ttatgcccag
     9901 ggcggtattg ccgccgtgtt tgatgaaact gacagcattg actcgcatgt ggaagacaca
     9961 ttgattgccg gggctggtat ttgcgatcgc catgcagttg aatttgtcgc cagcaatgca
    10021 cgatcctgtg tgcaatggct aatcgaccag ggggtgttat ttgataccca cattcagccg
    10081 aatggcgaag aaagctacca tctgacccgt gaaggtggac atagtcaccg tcgtattctt
    10141 catgccgccg acgcaactgg tagagaagta gaaaccacgc tggtgagcaa ggcgctgaac
    10201 catccgaata ttcgcgtgct ggagcgcagc aacgcggttg atctgattat ttctgacaaa
    10261 attggcctgc cgggcacgcg acgggttgtt ggcgcgtggg tatggaaccg aaataaagaa
    10321 aaggtggaaa cctgccacgc aaaagcggtg gtgctggcaa ccggcggtgc gtcgaaggtt
    10381 tatcagtaca ccaccaatcc ggatatttct tctggcgatg gcattgctat ggcgtggcgc
    10441 gcaggctgcc gggttgccaa tctcgaattt aatcagttcc accctaccgc gctgtatcac
    10501 ccacaggcac gcaatttcct gttaacggaa gcactgcgcg gcgaaggcgc ttatctcaag
    10561 cgcccggatg gcacgcgttt tatgcccgat tttgatgtgc gcggcgaact ggccccgcgc
    10621 gatattgtcg cccgcgccat tgaccatgaa atgaaacgcc tcggcgcaga ttgtatgttc
    10681 cttgatatca gccataagcc cgccgatttt attcgccagc attttccgat gatttatgaa
    10741 aagctgctcg ggctggggat tgatctcaca caagaaccgg taccgattgt gcctgctgca
    10801 cattatacct gcggtggtgt aatggttgat gatcatgggc gtacggacgt cgagggcttg
    10861 tatgccattg gcgaggtgag ttataccggc ttacacggcg ctaaccgcat ggcctcgaat
    10921 tcattgctgg agtgtctggt ctatggctgg tcggcggcgg aagatatcac cagacgtatg
    10981 ccttatgccc acgacatcag tacgttaccg ccgtgggatg aaagccgcgt tgagaaccct
    11041 gacgaacggg taataattca gcataactgg cacgagctac gtctgtttat gtgggattac
    11101 gttggcattg tgcgcacaac gaagcgcctg gaacgcgccc tgcggcggat aaccatgctc
    11161 caacaagaaa tagacgaata ttacgcccat ttccgcgtct caaataattt gctggagctg
    11221 cgtaatctgg tacaggttgc cgagttgatt gttcgctgtg caatgatgcg taaagagagt
    11281 cgggggttgc atttcacgct ggattatccg gaactgctca cccattccgg tccgtcgatc
    11341 ctttctcccg gcaatcatta cataaacaga taaaaagcct gggttagcgc cgtatacgct
    11401 tcggaatagt tctggtctgg cccacgaatg actaagcgat cgctaaagca ttctcccgcc
    11461 tgcggggaga atgccagcag cacccgatgc ggcagtcgcg cttcgttttc cgccacatcc
    11521 gtccgcaaac gtaaatgcca gcccatactt aatgccagct ccgtaaaact attaccaatc
    11581 tgctctggca gcaccacgca gaaaaatccc tcttcggtaa tgcactccgc cgcgcaagta
    11641 agtaaagtct ggtgatcaaa tgaggtggtg tagcgcgcct gttcccgctg cggcgtcgcg
    11701 cactccactc cttgctcata atacggcggg ttgctgatga ttaaatcaaa gcgcgcggtc
    11761 tgctgtgtta cccactgctg aatatccgcc gtatggacgt taattctctc taaccacggg
    11821 gactgggcga cattttcctg cgcctgagtg gcagcttcac tttccagctc aacggcatca
    11881 attatcacgc tgtcactggt tcgctgcgcc agcatcagtg ccagcaagcc actacccgca
    11941 ccgatatcaa ggcaacgttt taccccagcc accggtgccc atgcgcccag caatatgcca
    12001 tccgttcccg ctttcatcgc acagcgatcg tgagcaacaa aaaactgttt aaaagtaaat
    12061 ccattacgac gaagcacgga tgtagactgt gacatgaaaa taaaaccttg caggaaaaac
    12121 ggcgatagca ccgggtgaga acaatacccg agaagcgata tccatacaaa cagatgaaga
    12181 ttgcagccgt aacgtctata atcagcgccc cacacagagg tagaacatga ctgtaacgac
    12241 tttttccgaa cttgaactcg acgaaagcct gctggaagcc ctccaggata aaggtttcac
    12301 tcgcccgacc gccattcagg ctgccgccat tccgcctgcg ctcgatggcc gtgatgtact
    12361 cggttctgcg ccgacaggca ccggtaaaac ggcggcgtat ctgctgccag cgttgcagca
    12421 cctgctcgat ttcccgcgta agaaatccgg tccgccgcgt attttgatcc tcaccccaac
    12481 tcgcgagctg gcgatgcagg tggccgatca tgcccgcgaa ctggcgaaac atacgcatct
    12541 ggatatcgcc accatcaccg gcggcgtagc ctatatgaac cacgcggaag tgttcagcga
    12601 aaatcaggac atcgtggtcg ccacgaccgg acgtctgctg caatacataa aagaagagaa
    12661 cttcgattgc cgcgcggttg aaacgctgat cctcgacgaa gcagaccgta tgctggatat
    12721 gggcttcgct caggatatcg aacatattgc tggcgaaacg cgctggcgta aacagaccct
    12781 gctcttttcg gcaacgctgg aaggcgatgc gattcaggac tttgccgagc gtctgctgga
    12841 agatccggtg gaggtttctg ccaatccgtc cacccgtgag cgcaaaaaaa ttcatcagtg
    12901 gtattaccgc gccgatgacc tggaacataa aaccgcattg ctggtgcatc tgttaaaaca
    12961 gccggaagcg acccgctcaa ttgtgtttgt gcgtaagcgt gagcgtgtgc atgagctggc
    13021 aaactggctg cgcgaagcgg gcatcaacaa ctgctatctc gaaggtgaga tggtacaggg
    13081 caagcgtaac gaagcgatca aacgtttgac cgaaggtcgc gtaaacgtac tggttgcaac
    13141 cgatgttgcc gcgcgcggta tcgacattcc tgacgtcagc cacgtcttta acttcgatat
    13201 gccgcgcagt ggcgatactt atttgcaccg tatcggacgt accgcgcgcg ccggtcgtaa
    13261 aggcaccgca atttcgctgg tagaagccca tgaccatctg ctgctgggca aagttggtcg
    13321 ctatattgaa gagccaatta aagctcgcgt aattgatgag ttacgcccga aaacgcgtgc
    13381 accaagcgag aagcagaccg gcaagccatc gaagaaagta ctggcaaaac gcgccgagaa
    13441 gaaaaaagca aaagagaaag agaagccgcg ggtgaaaaaa cgccatcgcg ataccaaaaa
    13501 tattggtaag cgccgtaaac caagcggaac gggcgtgcca ccgcaaacga cagaagagta
    13561 atctcaatgc cgggtttaag cccggcatta tttttatccc ggcaaaaaac aatcttcaac
    13621 acactgaata aaagtgtgca tcgccgggct aaccgctttt ccggcatggt gagcgcacat
    13681 tgccgtaatg gtctgcgagt gttcgccaaa aggcaattca attaattctc cgctctcaag
    13741 ctctttttcc accgcaaagc ggggcaagta actgacaccg atattcgccg cgacacaacg
    13801 tttgatactt tcaatgctta acagctcaat ggtgttttcc accgtgatcc gccgctggcg
    13861 cagcgtgctc tcaaatatct gccggaatac acattgcggt tcgttgataa taaagctaca
    13921 ggcgttatgt cttcccggct cagtaaaatc gacatctgca atttgcggtg aagctaccag
    13981 caccagcgat tgctcaccca attctcgtcg gttcagggca tcatcattcc ctacgcgata
    14041 aaaaaccccg acatcggcct catcattcaa tagcgcatca cggatcacgt aacagttcag
    14101 agactgcaac gataaacgca cttttggggc gcgttgccga aaacgctgca acacctgcgg
    14161 cattcggtaa gagagcaacg tttcgcccga aacaacgcgt agttccccgt ccggatccga
    14221 ctcctttttg gcagcctcac gaagggtatc catcactctg gtgagttcat aaatgtgcgg
    14281 cagcagcttt ttaccttcgc gagtgaggca catccgtcgg ccaattttct caaataactg
    14341 taccgagaac tcctgctcaa gctgctgaat atgaaaagtc accgtcgatt gtgtacagca
    14401 caatttttgc gaagctcgca aaaaggaacc ctcttccacc acggttttaa gcgtaataaa
    14461 acggcgcaga tccataaccc caaacctatc gaaaatatcg aatttagaat gta
//
LOCUS       DBFIFM010000118        14475 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.118, whole genome shotgun sequence.
ACCESSION   DBFIFM010000118 DBFIFM010000000
VERSION     DBFIFM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.118"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..234
                     /locus_tag="N7C74_003036"
     CDS             <1..234
                     /locus_tag="N7C74_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432860.1"
                     /translation="KVPLTAQLIAKYKMDDQLDQLLVTREIQDEIKSKIQDKIDELSK
                     NLFNTMTETIENNFDDIFRQQSENMSNYYEFVD"
     gene            complement(412..927)
                     /gene="pgpA"
                     /locus_tag="N7C74_003037"
     CDS             complement(412..927)
                     /gene="pgpA"
                     /locus_tag="N7C74_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HGU2432861.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGIL"
     gene            complement(905..1882)
                     /gene="thiL"
                     /locus_tag="N7C74_003038"
     CDS             complement(905..1882)
                     /gene="thiL"
                     /locus_tag="N7C74_003038"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HGU2432862.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVVPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(1960..2379)
                     /gene="nusB"
                     /locus_tag="N7C74_003039"
     CDS             complement(1960..2379)
                     /gene="nusB"
                     /locus_tag="N7C74_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HGU2432863.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(2399..2869)
                     /gene="ribE"
                     /locus_tag="N7C74_003040"
     CDS             complement(2399..2869)
                     /gene="ribE"
                     /locus_tag="N7C74_003040"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HGU2432864.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(2958..4061)
                     /gene="ribD"
                     /locus_tag="N7C74_003041"
     CDS             complement(2958..4061)
                     /gene="ribD"
                     /locus_tag="N7C74_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HGU2432865.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDVQTQAL
                     YPQHHLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(4065..4514)
                     /gene="nrdR"
                     /locus_tag="N7C74_003042"
     CDS             complement(4065..4514)
                     /gene="nrdR"
                     /locus_tag="N7C74_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HGU2432866.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            4665..5204
                     /locus_tag="N7C74_003043"
     CDS             4665..5204
                     /locus_tag="N7C74_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HGU2432867.1"
                     /translation="MNINVFRLILLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            5503..6387
                     /gene="tsx"
                     /locus_tag="N7C74_003044"
     CDS             5503..6387
                     /gene="tsx"
                     /locus_tag="N7C74_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HGU2432868.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(6564..6911)
                     /gene="yajD"
                     /locus_tag="N7C74_003045"
     CDS             complement(6564..6911)
                     /gene="yajD"
                     /locus_tag="N7C74_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HGU2432869.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(6999..7280)
                     /locus_tag="N7C74_003046"
     CDS             complement(6999..7280)
                     /locus_tag="N7C74_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU2432870.1"
                     /translation="MYKLSGKAVEDFRGIYDYTLGKFGDEQADRYTDSLGTFLDTLSQ
                     MPEIGQDYDAIPEVKKIAFRFHTVYYVIRVDDILIARILHQLMEPRRHW"
     gene            complement(7273..7515)
                     /locus_tag="N7C74_003047"
     CDS             complement(7273..7515)
                     /locus_tag="N7C74_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HGU2432871.1"
                     /translation="MRKITSVSVGEQLDSFITRMVQSGRYGSASEVMRSALRLLEQQE
                     SRDEAVRNAVIEGLESGESSMTLRDIAAERKQKHRV"
     gene            complement(7667..8638)
                     /gene="secF"
                     /locus_tag="N7C74_003048"
     CDS             complement(7667..8638)
                     /gene="secF"
                     /locus_tag="N7C74_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HGU2432872.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(8649..10496)
                     /gene="secD"
                     /locus_tag="N7C74_003049"
     CDS             complement(8649..10496)
                     /gene="secD"
                     /locus_tag="N7C74_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HGU2432873.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(10524..10856)
                     /gene="yajC"
                     /locus_tag="N7C74_003050"
     CDS             complement(10524..10856)
                     /gene="yajC"
                     /locus_tag="N7C74_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HGU2432874.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(10879..12006)
                     /gene="tgt"
                     /locus_tag="N7C74_003051"
     CDS             complement(10879..12006)
                     /gene="tgt"
                     /locus_tag="N7C74_003051"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HGU2432875.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(12061..13131)
                     /gene="queA"
                     /locus_tag="N7C74_003052"
     CDS             complement(12061..13131)
                     /gene="queA"
                     /locus_tag="N7C74_003052"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HGU2432876.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            13224..13805
                     /gene="acpH"
                     /locus_tag="N7C74_003053"
     CDS             13224..13805
                     /gene="acpH"
                     /locus_tag="N7C74_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HGU2432877.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYAALETRFWQFYPRMMAQASRKAL"
     gene            complement(13826..>14475)
                     /locus_tag="N7C74_003054"
     CDS             complement(13826..>14475)
                     /locus_tag="N7C74_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432878.1"
                     /translation="PVDIDGVSLKSITREKIAEEIAKKTAEIIAKQNAKSIEEKNIEK
                     NKKQTNSEDNFVLKFSPEEINEMLLDYSDEEQKKLRPFFTSDHIYCLFDAFSQGLHNV
                     ARNGSPRYAGLLRQIASKSAILQDFDQQLFWRTFTGGGLHRHISVKSEPEKTDVWRFS
                     SQFDFPHINENGELSRTVLMFEGVAESNFKDIANHSSECFSYRCYAAPLDANAKK"
BASE COUNT         3627 a         3861 c         3444 g         3543 t
ORIGIN      
        1 aaagtacctt taactgcaca attaattgca aaatacaaaa tggatgatca attggaccaa
       61 cttttggtta cccgtgaaat tcaagatgaa ataaaatcaa agattcaaga caaaatagat
      121 gaactttcta aaaatttatt caacactatg accgaaacca ttgagaataa ttttgatgat
      181 atttttcgtc aacagtcaga gaatatgagc aattattatg agttcgtcga ctgacgatat
      241 ttcagtgtca ttgaattatg agtgacaaag atacaccttg aaatatcgcc gcatagtata
      301 ttcggcgatg tcgggtttta ctttaacaga gtgccccaaa attggggcac tgttcattta
      361 taaatcaaac cgggtggtct ggaatcgcct acacctcccg gtcaatggca tttaaagaat
      421 acccagcggc cagtgatgac cgataaagta caggatgcct gcggaaatca ccccggcgac
      481 aatatcatcg atcatgatcc ccatgccgcc atgtacattg cgatcaaacc agcggatcgg
      541 ccacggcttc cacatatcca gaatacggaa aatcacaaac ccggcggcga cccactgcca
      601 gtcattggtc ggcagcgcca tcagggtgat ccacatgcca ataaactcat cccatacgat
      661 gctaccgtga tcgtgcaccc ccatgtcttt cgccgtttga tgacaaagat agacgccgat
      721 acagatcccc aacatcacca ccagcgagta gagctgccag ggcaaaaagg tcatcagata
      781 ccagaacgga atcgccgcca gcgatcccat cgtgccagga acgattgggc ttaatccact
      841 tccgaatccg acagcaagca gatgccacgg attactcatc ttcaggcgac ttttcgcgac
      901 atctttatgg cgtggcaaaa tggtcatatc ctttccagtc taacgtgaca ggttcgccgt
      961 cacgaataaa acaaagccct tcgatatcgg cggtcatttg cccgatacag gtaaacggta
     1021 cgcccagatg accgagagcc acatccagcg cgccacggtt cagttccggc accgtgaaac
     1081 acaactcgta atcttcaccg ccagagagcg cccagcgcag cgcctgttcc ggtacaacat
     1141 ggcgagaaag cgcatcagaa aacggcagca atgccaggtc aatacgtgcg ccgcagtcgc
     1201 tggctttcac gatatgcccg agatcggaaa tcagaccgtc agagagatcg atagctgaat
     1261 ttgccagatc gcgcagtgcc tgcccctgta aaatacgcgg cgatggacgg agatgacgtt
     1321 tgatcaagta gtccgcatct ttagcatcgg caacctgcaa acggttttgc aaaatcgcca
     1381 gcccggcggc gctatcgccc ggtgtaccgg tcacatagat ccagtcaccc ggtttcgccc
     1441 cagagcgcgt taaggctcgt cccatcggaa caaagccgtg gatacccaac gtcattgata
     1501 atggcccacg cgtggtatcg ccgccaatga gttgcatatc gtaataattg agaagatcaa
     1561 acaaactgtc gctgaaggac tcaagccacg cttcgtctac gtccggtaag gttaatgcca
     1621 gcgtcagcca ggccggatcg gcccccatcg ctgccagatc gcttaggttc accgccagtg
     1681 ctttataagc cagatcagca ggatcgatat cagggaggaa atggttgccc gccaccagcg
     1741 tatcagtgct gatcgccagg gtctgtttct cggggatatt gagaagagcg caatcgtcgc
     1801 cgatgcccag ttcgacatca agacgagaac ttcttacacg gtcaaaataa cgggcaatca
     1861 gggagaactc gccacatgcc atacgttatg cctcagcagg taaaaaagaa aaggccggcg
     1921 atcgcggaac agtcttccgt gaatctaccg gcctggatat tactttttgt tagggcgaat
     1981 cacaggtgct gctttatcga gtacgccgtt gacgaactta tggctgtctt ctgcgccgaa
     2041 cgatttcgcc agttcgatcg cttcgttaat ggccactttg tatggcacat cgctacgttt
     2101 agacagttcg tacagcgcaa tgcgcagtac tgctttttct acctgtccca gttcttccag
     2161 caggcgggac aggtatggct tcatcagtcc gtcgaggtat gcggtattag tcgccacccc
     2221 ggccagcagc tcacggaagt acaggacgtc aacgtctttt acatcctgtt cagccaggaa
     2281 ctggtattca acatcagcga tgtcgttctg ggacaactgc caggagtaga gcgcctggac
     2341 ggcacactca cgagcgcggc gacgagcagc aggtttcacg gatttcccct tactaatttc
     2401 aggccttgat ggctttcaat acattaatca tttcaagcgc ggtcagtgca gcttctgcac
     2461 ctttgttgcc agctttggtg ccagcacgtt cgatcgcttg ttcaatgctt tcagtggtca
     2521 gaaccccaaa agcaaccgga atttcgctgt cctgggcaac atgcgccagg ccgttgcttg
     2581 caccaccagc gacatattca aaatgggcag tgccaccacg aataaccgta cccagcgcaa
     2641 tcaccgcgtc gtatttaccg gttttagcca gtgcacccgc cgccagcggc agctcatagg
     2701 caccaggcac ccaaacaacg gtaatgtttt catcttttac ctgaccgata cgtttcagtg
     2761 catcaattgc accttccagc aggctgtcat tgataaagtt gttgaaacgc gcgatggtga
     2821 tggcgacgcg agcgtccggg gtagcaacgt tagcttcaat aatgttcatt ttcttccttc
     2881 gggttcgagt atggccccgc aggggggcgg attttagcat aatatttcgt gcgctgcttc
     2941 cctttcgagc cgggagatca tgcacccact aaatgcaggc aaacatccgg gcctacatga
     3001 cgtatctctt tgaatttaaa ttggggggcg tcagctaatt tctcaagccc tggcagtgtg
     3061 cataatccac gggcgtcgct gcctaatagt ttaggtgcga tatagacaat cagctcatcg
     3121 actaaccccg cctgcagcaa tgcgccagcg agcgttggcc ccgcttccac ccagatgctg
     3181 ttaatctgct gtttacccag ttgcatcatc agtacaacca gatccagatg acctttatgc
     3241 tctggaatca gcaaggtacg caccgtttcc ggccactcac gagaatcttc ctgcgtacgc
     3301 gcgaaccagg tttcgccggg ctgctgcaca atgtgatgtt ccggcgtcac gcgattttgg
     3361 ctatcaatca caatacgtac cggctggcgg agatgatgtt gcgggtagag cgcctgagtt
     3421 tgtacatcca gttcagacca acgcaccgtt aaggcaggat catccgccag caccgtagcg
     3481 ctgctggtta aaatggcatg actttgtgcg cgcagtcgtt gtacgtcgcg ccgcgcctga
     3541 ggcgaagtga tccactggct ttcgccgctc gccatcgccg tgcggccatc aagcgatgcg
     3601 ccaagtttta actgaatata aggaaagccg gtgcgcatcc gcttgagaaa gcctttattc
     3661 aattgctcgg cttcactcat catcaggccg tggctgacgt caatgccagc ctgttgcaga
     3721 cggtaaagtc cacgcccagc gacctgcggg ttcgggtcct gcatcgcagc gaccacgcgc
     3781 gctacgccag cggcgatgag tgcgtcacag cacggtggcg tacgaccatg atggctacag
     3841 ggttcgagtg tgacataggc ggtcgcacct ttggcttttt cacccgccat acgcaacgcg
     3901 tgtacttcgg catgtggttc acccgctcgt tggtggtaac cttcaccgac aatttcgcca
     3961 tctttgacaa tgacgcaccc gacattcggg ttggggtggg tggtaaaacg tcctcgttgc
     4021 gccagcttta gcgcccgcgc catgtaatac tcgtcctgca cggcttagtc ctccaggcgc
     4081 gcgatctctt cgccaaattc tttgatatct tcgaaactgc ggtagacaga ggcaaaacgg
     4141 atataggcga ctttatcgag ctttttcaat tgctccatca ccagattgcc aatcatcttg
     4201 ctcggcactt cgcgctcacc ggtggcgcgc agctgcgatt taatatgatt gattgccatt
     4261 tcgacgtcat cggaactcac cggacgtttt tccagcgccc gcagcattcc gctacgtaat
     4321 ttttcttcat taaacggttc acgcacgtcg ttgcttttta caacacgcgg cataaccagc
     4381 tccgccactt caaaggtggt gaaacgttca ttacacacca gacactgccg acggcggcgt
     4441 acggatgaac cctcgcccac gagacgagag tcaattacct tagtgtccac ggcgaaacag
     4501 aatgggcaat gcatacggcg tcctgaccag gtggttaacg gaaatctatt ttaccctgaa
     4561 ctgacgatac aacaaaggcg caacgctttt gagataaagg atcgatgctt tcgctgcttt
     4621 taccctctac cattaggcta attgcgacga gaaagggacg acctatgaat attaacgttt
     4681 ttcgactgat cctgctggga agcctattca gccttagcgc ctgtgtgcag caaagtgaag
     4741 tgcgacagat gaaacacagc gtcagcacgc tgaaccagga gatgacgcaa ctcaatcaag
     4801 aaacagtcaa aatcactcag caaaacaggc tgaatgcaaa atccagcagt ggggtttacc
     4861 ttctgcctgg agcgaaaaca ccggcaagac tggaaagcca gatcggtact ttacgtatgt
     4921 cgctggtgaa tattacgcct gatacagatg gcaccaccct gacactacgt attcagggtg
     4981 agtcgaatga tcccttgccc gcattcagcg gaactgttga atatgggcaa attcagggaa
     5041 caatagacaa ctttcaggaa atcaatgtac agaatcaatt gattaatgcc cctgccagcg
     5101 tcctcgcccc cagcgatgtt gatattccgt tacagttaaa ggggatttct gtggatcagt
     5161 taggctttgt acgtatccac gacattcaac ctgttatgca gtaaacttat agcggggcga
     5221 ttttgtgcgt cccgcaacat ccttccccat cattttgtta ctccgcttac ataacccaga
     5281 ttgatagtaa aagtttgcaa caagggcgaa agtcagtaca atccccgccc gaatgtgtgt
     5341 aaacgtgaac gcaatcgatt acgtaaatga tagaactgtg aaacgaaaca tatttttgtg
     5401 agcaatgatt tttataatag gctcctctgt atacgaaata tttagaaacg caatttgcgc
     5461 ctttttcact cccgcaaggg attttcaaac agtggcatac atatgaaaaa aacattactg
     5521 gcagccggtg cggtactggc gctctcttcg tcttttactg tcaacgcagc tgaaaacgac
     5581 aaaccgcagt atctttccga ctggtggcac cagagcgtta acgttgtcgg aagctatcac
     5641 acccgtttcg gaccgcagat ccgcaacgat acctaccttg agtacgaagc attcgctaaa
     5701 aaagactggt tcgacttcta tggttatgcg gatgcgccgg tattcttcgg cggtaactcc
     5761 gatgcaaaag gtatctggaa ccacggttct cctctgttta tggaaatcga accacgtttc
     5821 tccatcgaca agctgaccaa tactgacctt agcttcggtc cgttcaaaga gtggtacttc
     5881 gcgaacaact acatttacga catgggtcgt aataaagatg gtcgccagag cacctggtac
     5941 atgggtctgg gtaccgatat cgacactggc ctgccgatga gcctgtccat gaacgtctat
     6001 gcgaaatacc agtggcagaa ctatggcgca gcgaacgaaa acgagtggga cggttaccgt
     6061 ttcaaaatta aatactttgt gccgattacc gatctgtggg gcggtcagct gagctacatc
     6121 ggcttcacca acttcgactg gggttccgat ttaggggatg acagcggtaa cgcaatcaac
     6181 ggtattaaga cccgtactaa taactctatc gcttccagcc atattctggc tctgaactac
     6241 gatcactggc actactctgt cgtagctcgt tactggcacg acggtggtca gtggaacgac
     6301 gatgcagaac tgaacttcgg caacggcaac ttcaacgttc gctctaccgg ctggggtggt
     6361 tacctggtag taggttacaa cttctgatta tgaaaatgcc gggatttatt cccggcattt
     6421 cagattgtta acaacgtacg ctttgttcat gccggatgcg gcgtaaacgc tttgtccggc
     6481 ccacaaaagc acgtaaattc aatatattgc tgaaataacg taggcctgat aagcgtagcg
     6541 catcaggcaa ttttacgttt taatcacttc ttcttgttca tcatcgcttt caggtcagcg
     6601 aatgggttgt acttcgcttc accgacatct ttctgcgcat cttcccctgc gataacggtc
     6661 gtaccatact gatccgcttc ggtatatttc gaatgctcat gatcgtggca atagagacaa
     6721 agcaattccc agttactgcc atcttccggg ttattggtat ggtcgtgatc aatgtggtga
     6781 acggtaagtt cacgcaggtt ggaataaaca aactcgcggg aacagcgacc gcagacccac
     6841 ggatagattt ttaatgcttt ttcgcgatag ccgctttcta accgcgcata gttttttggg
     6901 atgatagcca tgaaaagtta cctggatgaa atgtagggtt acgcgcagtt tcccaagatg
     6961 cggggatttt cgcaatcctg agtcaagaaa ttctcacatt accagtgacg gcgaggttcc
     7021 atcaactgat gaagaatgcg cgcaatgaga atgtcatcga ctcttataac gtaataaaca
     7081 gtgtgaaacc taaatgcgat cttctttacc tctggaatcg catcatagtc ttgccctatc
     7141 tcgggcattt gagagagtgt atccagaaaa gtgccgagag agtcggtata gcgatccgcc
     7201 tgctcatcac caaactttcc cagcgtgtag tcatagatcc ctctgaaatc ttcaacagct
     7261 tttccgctaa gcttatacac gatgtttttg cttcctttct gctgcgatat cacgaagcgt
     7321 catcgaactt tcaccgctct caagaccttc aataacagca tttctgacag cttcatccct
     7381 ggattcttgt tgttccaata gacgaagtgc cgaacgcatt acttcactgg ccgagccata
     7441 acgaccagac tggaccatcc gggtaataaa actatctaat tgttcgccaa cgcttactga
     7501 ggtaattttg cgcataagcg atctccacgt attaactatt aatacaaagt atatgtcaga
     7561 acgctttcta caaccaaacc gtattcagtt gcctgtgaaa caggcaaaaa aaaaccgcag
     7621 cgcaaatacg ccacggtttt tttaacagaa cggatttaaa acaccattac ggcagaattg
     7681 acggctgatc cgccccttct ttttccactt tctgctgcaa catgtgttcg cgcttcatac
     7741 ccagtttcag agccaacgca gatgccacat agatggaaga tgcagtaccg atggaaacac
     7801 cgataagcat ggtcagcgag aagccttcca gtaccggacc accgaagaga tacagcatca
     7861 ggataaccat caaggtagta ccggatgtga tcaaggtacg gtgcagcgtc tgggtcaagg
     7921 acacgttaaa gatttcgtaa ggcgtaccgc gacggatctt gcggaagttt tcacgaattc
     7981 ggtccgatac cacgatactg tcgttaagcg agtaaccgat aaccgacatc aacgatgcca
     8041 caatggtcag gtcaatctcg atatggaata acgacaaaat acccagcgta atgataacgt
     8101 cgtgcgccag cgcaataacc acccctgccg ccagtcgcca ctcaaagcgg aaacctacgt
     8161 acacgaggat agacagcagc gctgccatca acgccatcgc accggtttgc gcaaggtctg
     8221 cccccacgct cggaccgacg aactcaatac gcttcactgc tgcattctga ttggtggatt
     8281 cgttaatcac cttcagaacc tggctgccca gcacctgacc gccggtttcg ccttcagcag
     8341 gcggcatacg gaccatgatg tcgtggctgc taccaaagtt ttgcagcatc ggctcttcaa
     8401 aaccggcttt ttgcaatgca tcacgcatta cgtcaatttc agccggtttt tcgagcgtaa
     8461 tttcgataac cgtaccaccg gtgaaatcca gcccccagtt aaagccgcgc acgcccataa
     8521 taacgatagc agcgattaac agcagaccag agatgccaaa agcccagtag tcccagcgca
     8581 taaagtcata gactttacgg ccgtggttta gttgttcaac agtatattcc tgtgccacat
     8641 cgcactcctc agattgacag cttcttgacg cgcttgccgc catatagcag gtttacgatg
     8701 gcacgcgtac cgacaatcgc ggtaaacatc gacgtcgcca caccgatacc ggtagtaatc
     8761 gcgaaccctt taattgcccc ggtacccact gcgtacagga tgatgacttt aatcagcgtg
     8821 gtgatgttcg catcgaagat agaactgaat gcgccacgat aaccttcatc aattgcctgt
     8881 tgaacagtac gtccgttgct caactcttct ttaatacgtt cgttgatcag tacgttcgca
     8941 tcgaccgcta ccgcaagggt taagacgata cccgcgatac ctggcatact cagtgttgcg
     9001 cctggcaaca gcgacataat accgacgatc aagatcaagt tggcaatcag agcactggtc
     9061 gcaatcagac caaacttctt atagaagatg atcatgaaca gaatagaaac cagcagaccg
     9121 gcaaggcaag cctccagccc ctgctcaatg ttctgcatac ccagggtcgg gccaattgta
     9181 cgttcttcaa caatctgaat cggcgcgatc aacgcaccgg cacgcagcag cagtgacagc
     9241 tgacgggctt cgttcgggtt gttgatgccg gtgatacgga agctgttacc cagacgagac
     9301 tggatgttgg cgatgttaat cacctcttcc tgtttcacca gaaccgcacg accatttgca
     9361 tctttcttac cgctgtcttt gtactccaca aacagggttg ccatcggttt gccgatgttg
     9421 tccttggtga agttagacat aatgttgcca ccagcgctat cgagcgagat gttaacctgc
     9481 ggctggttgt attcgtcctg gctggaagtg gagtcggtga tatggtcacc ggtaagaatt
     9541 acgcgtttgt acagcacaac cggctgacct tcgcgggtct gtttcacttc agagtcgccc
     9601 ggtacgcgac cggatgccgc agcggcctga tcaacgttgg tgtttaccag acggaattcc
     9661 agcgttgccg tcgcacccag aatctctttc gcacgcgcag tgtcctgaat acctggcagt
     9721 tcaacaacga tacggtcagc accctgacgc tgaaccaccg gctccgccac gccaagttgg
     9781 tttacacggt tacgcaggat attgatgttc tgctgcaccg catattcacg cgcttcactc
     9841 agacgagcat cgctcattac cgcacgcagc tggttgctgc cctgactgct aatcaccagg
     9901 tccggatgac gcttgctcag atacgcaatg gcttcatcac gagctttagc atcgcggaaa
     9961 gtgatgctca ggccgtagtt attttcttta cgaacggtgg tatacgggat gcctttttcg
    10021 cgcaggtcgc tgcgcaggct atcgatattt tgttcctgga gtttgccaag cgcggtgtcc
    10081 atatccactt ccatcaggaa gtgaacgccg ccacgcaggt caaggccgag cttcatcggc
    10141 tcagcgtgaa tagctgccag ccagcgcggc gttgccgggg caaggttaag cgccacgacg
    10201 tatttgtcac ccataacgcc cattaatgct tcacgagcgc gcagctgggt gtcagtggag
    10261 tcgaagcgcg caagaatagc gccctcttcc agtgccacag acttagcagt tattttttct
    10321 tcttgtaacg ttttctggac ctggatcagc gtttgctcac tggcggcgac tccgcgcgca
    10381 ccagtgatct gaacagccgg atcctcacca aacaggttgg gaagcgcata cagcagaccg
    10441 atgacaatca ccacgatcag catgacgtac ttccacaaag gataacggtt taacacggca
    10501 attcccttag ggaaaaattt taattacagc gccttcatgg tgcctttcgg caggacggca
    10561 gctacgaagt cacgtttaat aactacttca gtggtgtcat tcagcgcgat agcaatgtag
    10621 ccgttttccg ctactttggt tacgcgacca accaggccac cgttcgtcag aacttcatca
    10681 cctttggcaa tggagtccat cagctttttg tgttctttgg tgcgcttctg ctgtggacgc
    10741 aggatcatga aatagaaaat cagaccgaat accaccagca tcaaaatcaa agacatcggg
    10801 ctaccttgcg ccggtgcacc cgttgccgct accgcatcag aaataaaaaa gctcattaaa
    10861 tttccctcat tattaatatt aatcaacgtt caaaggtggt acttctcgcc cctgacgctg
    10921 gtaaaaatca gttacgaagc tctctaattt accctcttca atagccttgc gtaaacccgc
    10981 catcaaacgc tggtagtaac gaaggttatg aatggtattg agtcgcgcgc ctaatatttc
    11041 gttgcaacgg tcaagatgat gcaagtaagc gcgtgaataa ttgcgacagg tgtagcaatc
    11101 acactcagga tcgagtgggc cagtatcgct cttatacttc gcattgcgga ttttcaccac
    11161 gccatcggtc acgaacaaat gaccatttcg ggcgttgcgg gttggcatta cgcagtcaaa
    11221 catatcgata ccacgacgta cgccttcaac caggtcttct ggtttaccaa cgcccatcag
    11281 gtaacgcggt ttgtctgccg gaatttgcgg gcatacatgc tccagaatgc ggtgcatatc
    11341 tgctttcggc tcacccacag ccagaccgcc gacagcgtag ccatcaaaac cgatatctac
    11401 cagaccttta acagaaatat cacgtaaatc ttcgtaaacg ctgccctgaa tgataccaaa
    11461 cagcgcattt ttgtttccga gactgtcaaa acgctcacgg ctacgcttcg cccaacgcag
    11521 agacatctcc atggagcgtt ttgcgtaatc ccagtcagca ggatacggcg tacactcatc
    11581 aaagatcatg acgatatccg aaccaagatc gtactgaatc tccatcgact tttccgggtc
    11641 gaggaaaatc gggtcgccgt tgatcgggtt acggaagtgc acgccctgtt cggtgatttt
    11701 acgaatatcg ccaaggctga agacttggaa gccgccggag tcggtgagga tcggtccctt
    11761 ccactgcata aaatcgtgca gatcgccgtg cagtttcatg atttcctggc ccgggcgcag
    11821 ccacaggtgg aaggtgttgc cgaggataat ttgcgcgcca gtggcttcaa cttcttccgg
    11881 cgtcatccct tttacggtgc cgtaggtgcc aacaggcata aaacaaggcg tttccactac
    11941 gccacgatca aagaccaggc ggccacggcg tgcgcgaccg tcggtggtgt ccagttcaaa
    12001 tttcattttt tctccactac gtcagaaaaa cagtccaacg tttaaaccag cgccgcggaa
    12061 ttactccccg acgcgctcat taattgcctg cggattgtac gtgataaaca tcgcatcacc
    12121 gtaactaaaa aagcgatatt tctcttctac cgccgcttta taggcattca ttgtgtgttg
    12181 ataaccggca aaggccgaaa ccagcataat cagcgtcgat tccggaaggt ggaagtttgt
    12241 caccagcgca tcgaccactt tgtactggaa gcccggatag ataaagattt gggtatcgtc
    12301 aaagaacggt tcgatgagat cgtttttcgc cgcctgagcc gcgctttcca gcgaacgtac
    12361 tgaggtggtg ccaaccgcaa tcacccggtt accgcgcgct ttcgccgcca gtaccgcgtc
    12421 taccacatcc tgcggtactt cagcgtattc cgagtgcatg atgtgatctt caatggtgtc
    12481 gacgcgtacc ggctggaagg tgcccgcacc aacgtgcagg gtgacgaacg ccatctccac
    12541 gcctttggcg cgtaattttt ccagcaaagg ttcgtcaaaa tgcagaccag cggtcggggc
    12601 tgcaaccgcg cccggttttt cgctataaac ggtttgataa agttcgcggt cagcgtcttc
    12661 gtccggacgg tcgatatacg gcggcagcgg catatggccg atgctgttga gaatatccag
    12721 caccgagcgt tcatcattaa attcgacttc aaacagtgcg ccgtggcgcg cggtcattgt
    12781 tgcgttaata ctttcgtcat cgcccagcag cagttctgcg ccaggttttg gcgctttcga
    12841 ggcgcgaata tgcgcaagaa tgcgtttgtc gtcgagcatc cgttcaacca gcacttcaat
    12901 cttgccgccg ctggctttac gcccaaacag gcgcgccggg atcacccggg tattattaaa
    12961 aaccagaaga tcgccggggt tgagcttatc gagtaaatcg gtgaaagtac cgtgcgtcag
    13021 cgcgcccgtc ggcccgtcca gcgacagtaa acgacagcta ctgcgttcag gcatcggata
    13081 gtgggcaatc agggattcgg gcaattcaaa ggagaaatcg gtaacgcgca tgacactgac
    13141 tcagactaaa ataagaggcg ggtagtctag tgccggggcg ctctccctgc aacctttacc
    13201 cccctttgga tagaatataa tcgatgaatt ttttagctca cctgcattta gcccatctcg
    13261 cggaaagctc gctttccggc aatttgctgg ctgatttcgt acgcggaaat cctgaagaaa
    13321 gttttccgcc cgacgtcgtg gctggcattc atatgcatcg acgtatcgac gtattgactg
    13381 acaatctgcc ggaagtccgc gaagcacggg agtggtttcg tagtgaaact cgccgcgttg
    13441 cgcctattac gctggatgtc atgtgggatc actttctttc ccgtcactgg tcgcagctgt
    13501 cgccggactt tccgctacag gaatttgttt gttatgcccg cgagcaagtg atgacaattt
    13561 tgccggactc accgccacgc tttatcaacc tgaacaacta cttatggtca gagcagtggc
    13621 tggtgcgcta tcgcgatatg gatttcatcc agaacgtgtt aaacggcatg gcaagccgtc
    13681 gcccacgtct ggatgccctg cgtgactcct ggtacgattt agacgcccat tatgccgccc
    13741 tcgaaacccg cttctggcag ttttatccac ggatgatggc gcaggcgtca cgcaaggcgc
    13801 tctgaaactt caaactccgg cgtcactatt ttttagcatt cgcatccagc ggggcggcat
    13861 aacaacggta agaaaaacat tcgctggaat gattggcgat atctttaaag ttgctttcag
    13921 caaccccctc gaacatgagt accgttctgg acaactctcc gttttcattt atatgtggaa
    13981 aatcaaattg gctactgaat cgccatacat ctgtcttttc tggctcgctc ttcacagaga
    14041 tatgtcgatg gaggccccct cctgtaaacg tgcgccagaa caattgttga tcaaaatctt
    14101 gtaatatcgc agacttggat gctatttgac gtagtaaacc tgcataccgt ggactgccat
    14161 tacgagcaac attatgcaaa ccctgactaa aagcatcaaa caaacaatag atgtgatcac
    14221 tagtgaaaaa aggtcttaat tttttttgtt cttcatcgct gtaatccagg agcatctcat
    14281 tgatttcttc cggtgagaat tttaagacaa aattatcctc agaatttgtt tgtttcttgt
    14341 ttttttcaat attcttctcc tcaatgctct tcgcattttg tttcgcaatt atttccgcag
    14401 tttttttcgc aatttcttcc gcaatttttt cccgagttat tgattttaga ctaacaccat
    14461 cgatgtcaac cggtg
//
LOCUS       DBFIFM010000119        14200 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.119, whole genome shotgun sequence.
ACCESSION   DBFIFM010000119 DBFIFM010000000
VERSION     DBFIFM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.119"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            122..1693
                     /gene="garD"
                     /locus_tag="N7C74_003055"
     CDS             122..1693
                     /gene="garD"
                     /locus_tag="N7C74_003055"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HGU2432879.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPKAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1842..2177
                     /gene="prlF"
                     /locus_tag="N7C74_003056"
     CDS             1842..2177
                     /gene="prlF"
                     /locus_tag="N7C74_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HGU2432880.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2177..2641
                     /gene="yhaV"
                     /locus_tag="N7C74_003057"
     CDS             2177..2641
                     /gene="yhaV"
                     /locus_tag="N7C74_003057"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HGU2432881.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(2696..3505)
                     /gene="agaR"
                     /locus_tag="N7C74_003058"
     CDS             complement(2696..3505)
                     /gene="agaR"
                     /locus_tag="N7C74_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HGU2432882.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3754..5034
                     /gene="kbaZ"
                     /locus_tag="N7C74_003059"
     CDS             3754..5034
                     /gene="kbaZ"
                     /locus_tag="N7C74_003059"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HGU2432883.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVESYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLNRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5057..5530
                     /gene="agaV"
                     /locus_tag="N7C74_003060"
     CDS             5057..5530
                     /gene="agaV"
                     /locus_tag="N7C74_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HGU2432884.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGIPTEPAVDLFKLL"
     gene            5541..6320
                     /gene="agaW"
                     /locus_tag="N7C74_003061"
     CDS             5541..6320
                     /gene="agaW"
                     /locus_tag="N7C74_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HGU2432885.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6310..7188
                     /gene="agaE"
                     /locus_tag="N7C74_003062"
     CDS             6310..7188
                     /gene="agaE"
                     /locus_tag="N7C74_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HGU2432886.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7206..7640
                     /gene="agaF"
                     /locus_tag="N7C74_003063"
     CDS             7206..7640
                     /gene="agaF"
                     /locus_tag="N7C74_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HGU2432887.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITSTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7637..8770
                     /gene="nagA"
                     /locus_tag="N7C74_003064"
     CDS             7637..8770
                     /gene="nagA"
                     /locus_tag="N7C74_003064"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGU2432888.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVSVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLSTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            9121..10275
                     /gene="agaS"
                     /locus_tag="N7C74_003065"
     CDS             9121..10275
                     /gene="agaS"
                     /locus_tag="N7C74_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HGU2432889.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALHSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFAVL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELHRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10288..11148
                     /gene="kbaY"
                     /locus_tag="N7C74_003066"
     CDS             10288..11148
                     /gene="kbaY"
                     /locus_tag="N7C74_003066"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HGU2432890.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11315..11791
                     /gene="agaB"
                     /locus_tag="N7C74_003067"
     CDS             11315..11791
                     /gene="agaB"
                     /locus_tag="N7C74_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HGU2432891.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            11863..12633
                     /gene="agaC"
                     /locus_tag="N7C74_003068"
     CDS             11863..12633
                     /gene="agaC"
                     /locus_tag="N7C74_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HGU2432892.1"
                     /translation="MAALVFVLGIDFWLEALFLFRPIIVCTLTGAILGDIQTGLITGG
                     LTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLPFSLLMQYVILF
                     FYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCTYLAQGAMQALV
                     KAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVCYIQVSNLLPVA
                     VLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            12623..13414
                     /gene="agaD"
                     /locus_tag="N7C74_003069"
     CDS             12623..13414
                     /gene="agaD"
                     /locus_tag="N7C74_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HGU2432893.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13415..14170
                     /gene="agaI"
                     /locus_tag="N7C74_003070"
                     /pseudo
     CDS             13415..14170
                     /gene="agaI"
                     /locus_tag="N7C74_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
BASE COUNT         3419 a         3590 c         3719 g         3472 t
ORIGIN      
        1 gacattgatc acatttctgc gtttaaactc ctgacattct tatttcaccc aatgaagtca
       61 tttattttta aatgagacca ggtcctcatt ttaataacct ctggctggag aatattgcac
      121 aatggccaac atcgaaatca gacaagaaac gccaactgcg ttttatataa aagttcacga
      181 cacagataat gtggcaatta ttgttaatga taatggcctg aaagcaggaa cgcgttttcc
      241 ggatgggctg gaattaattg aacatattcc ccaggggcat aaagtcgcat tgctcgacat
      301 tccggctaat ggtgaaatta ttcgttatgg cgaagtgatt ggttacgccg tgcgtgcaat
      361 cccacgcgga agctggatcg acgaatcaat ggttgtactg ccgaaagcgc cgccgttaca
      421 cacgctgcca ctggcaacca aagtcccgga acccttaccg ccgctggaag gatacacctt
      481 tgagggctat cgcaatgccg atggcagcgt gggcaccaaa aacctgctcg gcatcaccac
      541 cagcgtccac tgtgtggcag gcgtggtgga ctacgtagta aaaatcattg aacgcgatct
      601 gctaccgaaa tacccgaacg tcgatggcgt ggtggggctg aatcatttgt acggttgtgg
      661 cgtggcgatt aacgcaccgg cggcagttgt acctatccgt accattcaca atatttcgct
      721 gaatcctaac tttggcggcg aagtaatggt gattggcctg ggttgtgaaa agttgcagcc
      781 tgagcgcctg ctgactggaa cggatgatgt gcaagctatt ccagtagaaa gcgccagcat
      841 tgtcagtttg caggatgaaa agcatgtcgg ttttcagtcc atggtcgagg atattttgca
      901 ggtcgccgaa cgccatctac aaaaactgaa tcaacgtcag cgagaaacct gtccggcttc
      961 agaactggtt gtcggcatgc agtgcggtgg cagcgatgcc ttttctggcg taacggcaaa
     1021 cccggcggtt gggtatgcgt ctgatctact ggtgcgctgc ggcgcaacgg tgatgttctc
     1081 agaagtcacg gaagtgcgtg acgcgatcca tctgctgaca ccacgcgcag tgaacgaaga
     1141 ggtcggcaaa cggctgctgg aggagatgga gtggtacgat aactatctca atatgggaaa
     1201 aaccgaccgc agcgccaacc cttcgccggg caacaagaaa ggcggtctgg caaatgtagt
     1261 ggagaaagcg ctcggctcca ttgctaaatc gggtaaaagt gcaattgttg aagtgctgtc
     1321 gcccggtcaa cgcccgacta aacgcggatt aatttacgcc gcgacgccag ccagcgattt
     1381 tgtctgtggc acgcaacagg tggcttcggg tatcaccgtg caagtgttta cgaccggtcg
     1441 tggtacgccg tacggcctga tggcggtacc cgtcattaaa atggcgaccc gcaccgagct
     1501 ggcgaaccgc tggtttgatt taatggatat taacgcgggc actatcgcca ccggcgaaga
     1561 aaccattgaa gaggtgggct ggaagttgtt ccactttatt ctcgacgtcg ccagcgggaa
     1621 gaagaaaacc ttctcggatc aatggggact gcataaccag ctggcggtgt ttaacccagc
     1681 accggtgacc tgatttcttt tggaacgtcc ctcgcaaaac atggccttag tgccatgttt
     1741 ttattgttta aagcccccac gtccattaat aatgcatttg cattacctta gttcaagctt
     1801 ataatttgag cagaaaacag gctgtaaaag gacagtgaat catgcccgct aatgctcgct
     1861 ctcacgctgt actgaccact gaatcaaagg tcacgatacg cggacaaaca actatccccg
     1921 cgccagtgcg tgaggcctta aaactgaagc caggccagga cagcattcat tacgaaattc
     1981 tgcctggtgg gcaagtattt atgtgccgac tgggagatga gcaggaggat catactatga
     2041 atgcattttt gcgttttctg gatacagata tccagaacaa cccgcaaaaa actcgtccat
     2101 tcaacattca acaaggaaag aaacttgtcg ctggcatgga cgtcaacatt gatgatgaga
     2161 ttggcgacga cgaataatgg attttccaca aagggttaat ggttgggcgc tatatgctca
     2221 tccctgtttt caggaaacct acgacgcttt agttgccgaa gtcgagacat taaagggaaa
     2281 agatcctgaa aattatcaga gaaaagctgc cacaaagtta ttggcggtgg tccataaagt
     2341 gattgaggag catatcacgg tcaatccatc atcaccggca ttccgtcatg gcaagtcgtt
     2401 aggctctggg aaaaataaag actggtcacg ggtaaaattt ggtgctggtc gctatcgtct
     2461 cttctttcgt tacagtgaaa aagagaaagt catcattctg ggatggatga acgatgaaaa
     2521 cacgctgcgc acctacggta aaaaaacaga tgcctatacc gtattcagca aaatgttaaa
     2581 aagaggacat cctcctgccg actgggaaac cctcacccga gagacagaag aaacccattg
     2641 atggtgttca caatgcccgc cgaacgcatt cgtgttgacc agcgggcacc ctttctcact
     2701 ccccgaccag aatcacttca accccagcct ttcgcagccc ttccaggcta tccgcaggaa
     2761 tgccttcatc aacaatgatc atgtcgatac gttgagtatc aatgatctta tgtaaactgg
     2821 aacgattgaa tttactggaa tcggtgacca cgatgatccg ttccgcaact tcgcacatcc
     2881 ggcggtttaa acgggcttca tcttcattat gcgtgctgac gccgcgctcc agatcgatcg
     2941 catctacacc aagaaacagc atatcgaagt ggtaattttg cagcgattgc tcagcctgat
     3001 cgccgtaaaa agattgcgac tgacggcgca aatgcccgcc ggtcatcagc agctcaacgc
     3061 cttccgcttc cagcaacgca ttagccacgt tcataccgtt ggtcatcgca attacgtcag
     3121 tgtgcttgcg catcagacga gcaatctcaa aagtggtggt cccggaatcg aggatcaccc
     3181 gatgacctgg ctgaatcaac tcaacggcag ctttcgcaac gctgcgtttc attgcggtgt
     3241 tcagcgcgct tttatcttcc actgatggct cgactgacgg cgttgtgcta tcgcagatca
     3301 acgcgccacc ataggcacgc acagcgatcc cctgcttttc cagaaacgcc agatcgttgc
     3361 ggatcgtcac ggtagatacg ccatacaatg ccgacagatc gttaacctgc acactccctt
     3421 gctgtcgcag acgctgaatg atctgttctc gtcgctcgct ggtgcctgtc actcgcttct
     3481 cacctgaagc gtcggtatta ctcatagtaa gtcctttcgt aaaactttcg tttcatttcg
     3541 ttttgcctat taacgccttt ctattaagca aatgcaagcc caccttgccc attgacgcaa
     3601 gctactctcg tttcagtgac tttcattatg tttcttttgt gaatcagatc agaaaactat
     3661 tatctttcgt tttattttta tctcaccatg acgcagtatc aactgaaaca aaacgaaaga
     3721 ttaatatcgc agtaatctga actggagagg aaagtgaaac atctgacaga aatggtgaga
     3781 cagcacaaag cgggcaaaac aaatggaatt tatgccgttt gttccgcaca tccgctggtg
     3841 ctggaggctg caatccgcta cgccagtgca aaccaaacgc cgttactgat tgaagcaacc
     3901 tccaatcagg tagaccagtt cggcggttat accggaatga cgcccgccga ttttcgcggc
     3961 tttgtttgtc agctcgccga ctcgttgaat ttcccgcagg atgcgttgat tctgggtggt
     4021 gaccatctgg ggccaaaccg ctggcaaaac ctgccggccg ctcaggcaat ggccaatgcc
     4081 gatgatttga ttaaaagcta cgttgcggca ggattcaaaa aaatccacct tgattgcagc
     4141 atgtcctgtc aggacgatcc gattccctta actgatgaca tcgtggctga acgcgccgcc
     4201 cgtctggcga aagtggcgga agaaacctgt cttgaacact ttggcgaagc cgatctggag
     4261 tatgtcattg gtaccgaagt gccggtacct ggcggcgcgc atgaaacctt aagcgagctg
     4321 gcggtcacca cgccggatgc cgcccgcgcc acgctggaag cccatcgtca cgcctttgaa
     4381 aagcaaggtt tgaatgccat ctggccacgc atcattgccc tggtggttca acccggcgtc
     4441 gaattcgatc acaccaacgt tattgattat cagcccgcca aagcgaccgc cttaagccag
     4501 atggtcgaaa gctacgaaac gctgattttc gaagcgcact ctaccgatta tcaaacgccg
     4561 caatcgctgc gccagctggt gattgaccac tttgccattc tgaaagttgg cccggcgctg
     4621 accttcgccc tgcgtgaagc tctgttctct ctggcagcga ttgaagaaga actggtgcca
     4681 gcaaaagcct gttctggtct gcgtcaggtg ctggaaaacg tgatgctcaa ccgcccggaa
     4741 tactggcaaa gccactacca cggtgacggc aacgcgcgtc gtctggcgcg tggttatagc
     4801 tactcggatc gcgtgcgcta ttactggccg gacagccaga ttgatgacgc tttcgctcat
     4861 ctggtacgta atctggcgga ttcaccaatt ccgctgccgc tgatcagcca gtatctgccg
     4921 ctgcagtacg tgaaagttcg ctccggcgag ctgcagccaa cgccacggga actcattatc
     4981 aaccatattc aggacatcct ggcgcagtac cacacagcct gtgaaggcca ataagcaaaa
     5041 caaagaggaa cacgctatgc caaatattgt tttaagccgg attgatgaac gcttgattca
     5101 cggtcaggtc ggcgttcagt gggtcggatt tgcgggggca aatctggtgc tggtcgccaa
     5161 cgatgaggtt gccgaagatc cggtacaaca aaacctgatg gaaatggtac tggcagaagg
     5221 gatcgccgta cgtttctgga cgctgcaaaa agttatcgac aacattcatc gcgccgccga
     5281 tcgacagaaa atcctgctgg tttgtaaaac acccgccgat ttcctgacgc tggtgaaagg
     5341 tggcgttccg gtgaatcgca ttaacgttgg caatatgcac tacgccaatg gcaagcaaca
     5401 aatcgccaaa acggtttctg tggatgcggg cgatatcgca gcatttaacg acctgaaagc
     5461 cgctggggtg gaatgcttcg ttcagggcat cccgacagag cctgctgtgg acctctttaa
     5521 attactttga gggattcatc atggaaatca gtctgttgca ggcattcgcg ttgggcatta
     5581 tcgcctttat cgctggcctg gatatgttta acggcttaac ccatatgcac cgcccagtgg
     5641 tcctcggccc gttagtcggg ctggtacttg gcgatctgca taccggtatt ttaaccggcg
     5701 gtacgctgga actggtgtgg atggggctgg ccccgctggc gggcgcacag ccgcctaatg
     5761 tgattatcgg tactatcgtc ggcacggcgt ttgccattac tactggcgtg aaacccgatg
     5821 tcgcagtcgg tgtcgccgta cctttcgctg tcgcagtaca gatggggatt accttcctgt
     5881 tctcggtgat gtctggcgtg atgtctcgct gcgaccggat ggcggagaac gccgatactc
     5941 gcggcattga acgcgtaaac tatctggcac tgctggcact cggcactttc tattttctct
     6001 gcgctttcct gcctatctac ttcggcgcgg aacatgcaaa aaccatcatt gatgtcctgc
     6061 cgcaacgatt aatcgacggc ctcggcgtcg cgggcggcat catgccagca atcggttttg
     6121 ccgtgctgct gaaaatcatg atgaaaaacg tctacatccc ctacttcatc ctgggctttg
     6181 ttgccgcagc ctggctcaag ttaccggtgc tggctattgc tgccgccgcg ctggcgatgg
     6241 cgctgatcga tctgctgcgt aaatctcctg aaccgactca acctgcggca cagaaagagg
     6301 aatttgaaga tggcatctaa tcaaaccacc ctgccgaacg tcagtgaaaa cgaagaaaca
     6361 ttgctgactg gcgtcaatga aaacgtgtat gaagatcaga gcattggcgc ggagctgacg
     6421 aaaaaagata tcaatcgcgt cgcctggcgt tccatgctgt tacaagcgtc ttttaactac
     6481 gaacgtatgc aggcttccgg ctggttgtac ggtctgctgc ctgctctgaa aaagatccac
     6541 actaataaac gcgacctggc gcgcgccatg aaggggcata tgggtttctt caatacccat
     6601 ccgtttctgg tgacatttgt tatcggcatt atccttgcga tggagcgttc taagcaggac
     6661 gttaacagta ttcagagcac caaaattgcc gtcggtgcgc cgctcggcgg gattggcgat
     6721 gcaatgttct ggctaacgct actgccgatt tgtggcggga ttggagccag tctggcattg
     6781 caaggctcca ttcttggcgc agtcgtcttt attgtgctgt tcaacgtggt ccacctgggg
     6841 ctgcgttttg gtctggcgca ttatgcttac cgcatgggcg tggcggcgat tccactcatt
     6901 aaagctaata ccaaaaaagt cggccatgct gcgtctatcg ttgggatgac ggtaatcggt
     6961 gcgctggtgg caacctatgt tcgtttaagc accacgctgg aaatcacagc gggcgacgca
     7021 gtggttaagt tacaggctga tgttatcgac aaactgatgc ctgccttctt accgttggtc
     7081 tacaccctga ccatgttttg gttggtacgc cgcggctgga gtccgctgcg tctgattgca
     7141 gttaccgtgg ttctcggcat cgttggtaaa ttctgccatt tcctttaaat acaatgaggt
     7201 attagatgtt aagtattatt ttgacagggc atggcgggtt tgccagcggc atggaaaaag
     7261 caatgaagca aattctcggt gagcaatcgc agtttatcgc catcgatttt ccggaaacct
     7321 caagcaccgc gctgcttaca tcgcagcttg aagaggccat cgcccaactg gattgtgaag
     7381 acggcattgt ttttctgacg gatttgctgg gcggcacgcc gtttcgcgta gcttcaacgc
     7441 tggcgatgca aaaaccgggc tgtgaagtga tcaccagcac caatttgcaa ctgctactgg
     7501 agatggtgct ggagcgcgaa gggttaagcg gtgaagaatt ccgtgtacag gcgctggagt
     7561 gtggacatcg tgggctgacc agtctggtgg acgagttagg ccgctgccat gaagaatgtc
     7621 cggtcgagga agggatatga cacacgttct gcgcgccaga aggctgctga ctgaagaggg
     7681 atggctcgat gaccatcagt tgcgtattgc tgacggtgtc atcgcagcaa tcgaaccgat
     7741 tccagtgagc gtgactgaac gcgatgcgga actgctctgc cccgcttaca tcgacaccca
     7801 tgtacacggt ggtgcgggcg ttgatgtaat ggatgacgcg ccggatgtac tcgacaagct
     7861 ggcaatgcac aaggcacgcg aaggtgtcgg cagttggttg ccgactaccg taaccgcgcc
     7921 gcttagtacc attcatgcgg cgctgaaacg tattgctcaa cgttgccaac gcggcggacc
     7981 tggtgcgcaa gtgctgggga gttatctcga aggaccgtac ttcacgccgc agaataaagg
     8041 cgcgcatccg ccggagttgt ttcgcgagct tgaaattgcc gagctggatc aattgattgc
     8101 cgtttctcag cacaccttac gcgtggtagc gctggcaccg gaaaaagagg gggcattgca
     8161 ggccatccgc catcttaaac agcaaaatgt acgagtgatg ctggggcata gcgcggcgac
     8221 ctggcaacaa actcgcgccg cgtttgatgc tggtgccgac ggcctggtgc attgctataa
     8281 cgggatgaca ggtttacatc accgcgaacc gggaatggtt ggcgcgggat taacggacaa
     8341 gcgcgcctgg ctggaactga tagccgatgg tcatcatgtg catccggcgg caatgtcgct
     8401 gtgttgttgc tgtgcgaaag agagaatcgt actgatcacc gacgcgatgc aggcagccgg
     8461 gatgccggat ggtcgctata cgttatgtgg cgaagaagtg cagatgcacg gtggcgttgt
     8521 ccgtaccgcg tccggtgggc tggcgggcag tacgctgtct gttgatgcgg cagtgcgcaa
     8581 catggtcgag ttgacgggcg taacgcctgc ggaagccatt catatggcgt cgctgcatcc
     8641 ggcgcgaatg ctgggtgttg atggtgttct gggatcgctt aaaccgggca aacgcgccag
     8701 cgtcgttgcg ctggatagcg ggctgcatgt gcaacaaatc tggattcagg gtcaattagc
     8761 ttcgttttga tggtttgctc cattattggg ccttcacttc ccccgtaagg cctttctttt
     8821 tctttcgttt tgatctgtgc agcggtgtcg gatgcgacgc taacgcgtct tatccgacct
     8881 acagttggtg accgcaaggc cggataaagc gtttgcgccg catccagcaa tcccttttgc
     8941 ttcctttatc ttttctttca acgatcacgg atttcgtttt atttcctttt tctccattga
     9001 actttcggtt tcttttctat agattttaat caacgaaaga catcaccaag tgaaatgaaa
     9061 cgaaaggcaa gtgaaagcga caacgcccga cgtcaagttc atcagactaa ggattgagtt
     9121 atgccagaaa attacacccc tgctgccgcc gcaaccggta catggactga agaagagatc
     9181 cgccatcagc ctcgcgcatg gatccgttcg ctcaccaaca tcgacgcgct acattccgcg
     9241 cttaataact tccttgaacc gttactgcgc aaagagaatc tgcggatcat cctgaccgga
     9301 gccggaacct cggcatttat cggtgacatc atcgcgccgt ggctcgccag ccataccggt
     9361 aaaaacttca gcgccgtacc gaccaccgat ctggtcacta atccgatgga ctacctgaac
     9421 ccagcccatc cgctgctgtt gatctccttc ggtcgatccg gcaacagccc ggaaagcgtc
     9481 gcagccgtgg aactggcaaa tcaatttgta ccggaatgct atcacctgcc gatcacctgc
     9541 aacgaagcgg gcgctcttta ccaaaacgcg atcaacagcg ataacgcgtt tgccgtgctg
     9601 atgcccgcag aaacgcacga tcgcggcttt gcgatgacca gcagcattac caccatgatg
     9661 gccagctgcc tcgcggtttt cgcacctgag acgatcaaca gccaaacctt ccgcgacgtg
     9721 gcggatcgtt gccaggcgat cctgacctca ctgggcgatt tcagcgaagg tgtgtttggt
     9781 tacgcaccgt ggaaacggat cgtttatctc ggcagcggtg gcttacaggg cgcagcacgc
     9841 gagtcggcgc tgaaagtgct ggaactgacg gcgggtaaac tggcggcctt ttatgattct
     9901 ccaaccggat tccgtcatgg accaaaatcg ctggtcgata acgaaacact ggtggtggta
     9961 tttgtctcca gccaccctta cacccgtcag tatgatcttg atctgctggc tgaacttcac
    10021 cgtgacaacc aggcaatgcg tgtaatcgcc atcgccgcgg aaagcagcga catcgtcgct
    10081 gccggtccac atatcatcct gccaccgtca cgtcacttta tcgacgttga gcaggcattt
    10141 tgcttcctga tgtacgccca gacgtttgca ctgatgcagt cgctgcacat gggcaatacg
    10201 ccggataccc catcagccag tggcaccgtt aaccgcgtgg tgcaaggcgt aatcattcat
    10261 ccgtggcagg cataagagga tcgcattatg agcattatct ccaccaaata tctgttacag
    10321 gacgcccagg ccaatggcta cgcggtgcct gcttttaaca ttcataacgc cgagacgatc
    10381 caggcgatcc tcgaagtgtg cagtgaaatg cgatcgccgg tgatcctcgc cggaacgccg
    10441 gggaccttta aacatatcgc gctggaagag atctacgccc tgtgcagcgc ctattccaca
    10501 acctacaaca tgccactggc gctgcatctc gaccaccacg aatcgctgga tgatattcgc
    10561 cgtaaagtcc acgcaggtgt gcgcagtgcg atgatcgacg gcagccactt cccgtttgcc
    10621 gagaacgtaa agctggtgaa atcggttgtc gacttctgcc actcccagga ttgcagcgtg
    10681 gaagcagaac tgggccgcct gggcggtgtt gaagatgaca tgagcgttga cgccgaaagt
    10741 gcattcctga ccgatccaca agaagctaaa cgctttgtcg aactgactgg cgtcgacagc
    10801 ttggcggtag cgattggtac ggcgcacggc ttatacagca aaacgccgaa gattgatttc
    10861 cagcggctgg cggaaattcg tgaagtggtg gatgttcctc tggtgctaca tggtgccagc
    10921 gatgttccgg atgaatttgt ccgtcgcact attgaacttg gcgtcacaaa agtgaacgtt
    10981 gccacagaat taaaaatagc cttcgccggc gcggttaaag cctggtttgc ggaaaatccg
    11041 cagggtaatg atcctcgtta ttatatgcgc gtcggaatgg atgcgatgaa agaagttgtc
    11101 agaaataaaa ttaatgtctg tggttcagcg aatcgaattt cagcataatc attagttttt
    11161 tatgatttat cccaatgtac ttcccgatta atatcgggga gtgccttaat ggaaaaggag
    11221 atgactaaac cttaataaat agatcagtac aatatcgcaa caataatata tttaaaaaaa
    11281 ttatattatt caactttatg gtgaggatta cacaatgagc agtccaaata ttctcttaac
    11341 ccgtattgat aaccgtctgg ttcacggtca ggttggcgtg acctggacat ccaccatcgg
    11401 tgcgaatctg ctggtagtcg tggatgatgt tgtcgctaac gatgatattc aacagaaatt
    11461 aatgggtatt accgcagaaa cctacggctt tggcattcgt ttctttacta tcgaaaaaac
    11521 cattaacgtc atcggcaaag ctgcaccgca tcagaagatc ttcctgattt gccgtacgcc
    11581 acaaacggta cgtaaattgg tagaaggtgg cattgacctg aaagatgtca acgtcggcaa
    11641 tatgcatttc tcggaaggga aaaagcaaat cagcagtaaa gtttatgtcg atgaccagga
    11701 tctcacggac ttacgtttta ttaaacaacg tggcgtgaat gttttcattc aggacgtccc
    11761 tggcgatcaa aaagaacaaa tccctgacta aatctaaaat cgccttaata ttggtttgag
    11821 gtaataaaaa tgcatgaaat aaccctactt tagggattat ccctggcggc gttagttttc
    11881 gttctgggga ttgatttttg gctggaagcc ttatttttat tccgcccgat aatcgtttgt
    11941 accctaactg gcgctattct cggtgatatt cagactggct taattaccgg tggtctgaca
    12001 gagttggctt tcgccggatt aacccctgca ggtggtgttc agccgcccaa cccgattatg
    12061 gcgggtctga tgaccaccgt cattgcatgg tctacgggcg ttgatgccaa aacggcaatt
    12121 ggtcttggcc tgccgtttag tttgttaatg cagtacgtca ttctgttctt ctattctgct
    12181 ttctcattat ttatgaccaa agccgataaa tgcgcgaaag aggcggatac ggcagcgttt
    12241 tcccgactta actggacaac gatgctcatc gtcgcttcag cgtatgcggt gattgctttc
    12301 ctctgtactt acctggcaca aggggcgatg caggcgctgg tgaaagcgat gcccgcttgg
    12361 ctgacccacg gctttgaagt ggctggcggt attctgcctg ccgttggttt tggcttgctg
    12421 ctgcgcgtga tgttcaaagc acaatatatc ccttacctga tcgccggctt cctgtttgtt
    12481 tgctacatcc aggtcagcaa cctgttgccg gttgccgtac tgggcgcagg ctttgcggtg
    12541 tatgagtttt tcaatgcgaa atcccggcag caagcgcaac cgcagcccgt tgccagtaaa
    12601 aatgaagaag aggactacag caatgggatc tgaaatcagt aaaaaagata tcacccgtct
    12661 gggctttcgt tcgtcgctgc tgcaagcgag ctttaactac gaaaggatgc aagcgggcgg
    12721 ttttacctgg gcgatgttgc cgatcctgaa aaagatttat aaggatgaca aaccgggcct
    12781 aagcgcagcg atgaaagata acctcgaatt tattaatacc cacccgaatc tggtcggatt
    12841 cctgatgggg ttattaattt cgatggaaga aaaaggagaa aaccgcgaca ccattaaagg
    12901 cctcaaagtg gcactgtttg gcccaatcgc cgggattggc gatgcgattt tctggtttac
    12961 tttgttgccg attatggcgg gaatttgctc atcatttgcc agccagggaa acctgctggg
    13021 gccgattcta tttttcgccg tttacctgct tatctttttc ctgcgcgtcg gctggaccca
    13081 cgtcggttat tcagtcggcg tgaaggcgat cgataaagtg cgagagaact cgcagatgat
    13141 tgctcgctcg gcaaccatcc tcgggatcac ggtaatcggc gggctgatcg cttcgtatgt
    13201 gcatattaac gtggtgacat cgtttgccat cgacagtacc cacagcgtcg cgctgcaaca
    13261 ggatttcttc gataaagtct tcccgaacat tttaccgatg gcctacaccc tgctgatgta
    13321 ttacttcctg cgggtgaaaa aagcgcatcc ggtgctgtta atcggcgtga cttttgtgct
    13381 ctctattgtt tgttccgcat tcggcatttt gtaaatggaa cgaggcactg cgtctggcgg
    13441 tgcctcttta cttaaggaat ttcatcctgt gcaaaccctt cagcaagttg aaaactatac
    13501 ggcgttaagt gaacgtgcca gcgaatattt attggccgtg atccgtagca aaccggatgc
    13561 cgtgatttgc ctggcgaccg gagccacgcc attactgacg tatcattatc tggtagaaaa
    13621 aatccactag cagcaggttg atgtcagcca gctcaccttc gtgaagctcg acgaatgggt
    13681 ggatctgcca ttaacgatgc ctggtacctg cgaaactttc ctgcaacagc atatcgtgca
    13741 gccgctgggg ctacgtgaag accagctcat cagctttcgc tccgaagaga taaatgagac
    13801 agagtgcgaa cgggtaacga acctgattgc gcgcaaaggc ggtctggatt tatgcgttct
    13861 cggattgggg aaaaacggtc atcttgggct gaacgaaccg ggagaaaacc tgcaaccggc
    13921 ctgccatatc agtcaacttg atgccagaac acagcaacat gagatgttaa aaaccgcggg
    13981 tcgccccgtg actcgtggga tcaccttagg cctgaaggat attctcaatg cccgcgaggt
    14041 tttgttactg gtgactggcg aaggaaagca ggatgcgaca gaacgttttc tcacggctaa
    14101 agtctctacc tctatcccgg cttcgttttt gtggctgcac agtaacttta tttgtttaat
    14161 taatacctaa ccagatgaaa aatttgctca aaatgtttaa
//
LOCUS       DBFIFM010000120        14054 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.120, whole genome shotgun sequence.
ACCESSION   DBFIFM010000120 DBFIFM010000000
VERSION     DBFIFM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.120"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            160..891
                     /gene="artJ"
                     /locus_tag="N7C74_003071"
     CDS             160..891
                     /gene="artJ"
                     /locus_tag="N7C74_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HGU2432894.1"
                     /translation="MKKLVLAALLASCAFGASAAEKINFGVSATYPPFESMDANNQIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPDRSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNSRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQQISDQWFPQ"
     gene            complement(942..2423)
                     /locus_tag="N7C74_003072"
     CDS             complement(942..2423)
                     /locus_tag="N7C74_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HGU2432895.1"
                     /translation="MRAIILLFDSLNKRYLPPYGDALTKAPNFQRLAAHAATFENSYV
                     GSMPCMPARRELHTGRCNFLHREWGPLEPFDDSMPELLKKAGIYTHLISDHLHYWEDG
                     GGNYHNRYSSWEIVRGQEGDHWHASVAQPPIPEVLRVPQKQTGGGVSGLWRHDWANRE
                     YIQQEADFPQTKVFDAGCAFIHKNHAEDNWLLQIETFDPHEPFHTTEEYLSLYEDNWD
                     GPHYDWPRGRVQESDEAVEHIRCRYRSLVSMCDRNLGRILDLMDEHDLWRDTMLIVGT
                     DHGFLLGEHGWWAKNQMPYYNEVANNPLFIWNPRSGVKGERRQALVQMIDWAPTLYDF
                     FQQPVPPDVQGQPLAKTVSHDEPVRSSAMFGVFSGHANVTDGRYVYMRAALPGREDDI
                     ANYTLMSCKMNSRYPVDEMRALSLAPPFRFTKGLQVLRIPAQEKYKGLNQFGHLLFDL
                     QNDPQQLHPIHDDVIESRMIALLIQLMKDNDAPPEQFQRLGLA"
     gene            complement(2435..2755)
                     /locus_tag="N7C74_003073"
     CDS             complement(2435..2755)
                     /locus_tag="N7C74_003073"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGU2432896.1"
                     /translation="MKDTINILFVCGYGVGSSVMLQTVVKKALTKYDFKVEMEHTAAG
                     EVGGFTDWADIFAISKKLLDVVSLDPKQGQYLIPIENIMDGETIGQQIYEVVQQHFPH
                     LINQ"
     gene            complement(2777..4120)
                     /locus_tag="N7C74_003074"
     CDS             complement(2777..4120)
                     /locus_tag="N7C74_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGU2432897.1"
                     /translation="MEGVPTMFAKFIDVIQTFLTEPAILIGLLVGIGYALDKKSPIKI
                     ITGMVSAMVGLMMVLFGGFQFSATFKPVAEAVSKAYGVHGYLMDSYAMKAATQIALGD
                     NFGYVGYVFVLAFFTNLLLVLFGRYTGAKGIFLTGNTGVSHSQAVLWLIVFWLGFSWT
                     TSIIIAGILTGVFWAFSTTLIVKPIAKVTKDAGFTIAHNQMLGLWFFSKFAHKFGDPE
                     KHDAENLKLPGWLAIFNHNVTAIAIVMTLFVGGFLLSTGIDNVQLMAKGKPWYIYIIN
                     LGLQFSMYMVILLQGVRMMVGEINGSFKGWQDRFIPNAIPAVDVAALLPFSPNAATLG
                     FVFCTFGTIFSMGILLLVHSPIMVLPGFVPLFFSGGPIGVLANRMGGYRSVIICTFLL
                     GIIQTFGTVWAIPLTGLAENGVGWTGIFDWATVWPAICEVLKFIAATFHLGPYAG"
     gene            complement(4400..5527)
                     /gene="rlmC"
                     /locus_tag="N7C74_003075"
     CDS             complement(4400..5527)
                     /gene="rlmC"
                     /locus_tag="N7C74_003075"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HGU2432898.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQA
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALVERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIALEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKM"
     gene            complement(5568..6056)
                     /locus_tag="N7C74_003076"
     CDS             complement(5568..6056)
                     /locus_tag="N7C74_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HGU2432899.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(6116..6961)
                     /gene="potI"
                     /locus_tag="N7C74_003077"
     CDS             complement(6116..6961)
                     /gene="potI"
                     /locus_tag="N7C74_003077"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HGU2432900.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAVISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     CARRG"
     gene            complement(6958..7911)
                     /gene="potH"
                     /locus_tag="N7C74_003078"
     CDS             complement(6958..7911)
                     /gene="potH"
                     /locus_tag="N7C74_003078"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HGU2432901.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNFGNFLQLTGDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(7921..9054)
                     /gene="potG"
                     /locus_tag="N7C74_003079"
     CDS             complement(7921..9054)
                     /gene="potG"
                     /locus_tag="N7C74_003079"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HGU2432902.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(9149..10261)
                     /gene="potF"
                     /locus_tag="N7C74_003080"
     CDS             complement(9149..10261)
                     /gene="potF"
                     /locus_tag="N7C74_003080"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HGU2432903.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQAPKIDRVRTRAWTKVKSGK"
     gene            complement(10612..11088)
                     /locus_tag="N7C74_003081"
     CDS             complement(10612..11088)
                     /locus_tag="N7C74_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HGU2432904.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(11176..12078)
                     /gene="rimK"
                     /locus_tag="N7C74_003082"
     CDS             complement(11176..12078)
                     /gene="rimK"
                     /locus_tag="N7C74_003082"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HGU2432905.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(12139..12861)
                     /gene="nfsA"
                     /locus_tag="N7C74_003083"
     CDS             complement(12139..12861)
                     /gene="nfsA"
                     /locus_tag="N7C74_003083"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HGU2432906.1"
                     /translation="MTPTIELTCGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPSSILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(12845..13132)
                     /locus_tag="N7C74_003084"
     CDS             complement(12845..13132)
                     /locus_tag="N7C74_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HGU2432907.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            13292..13549
                     /gene="grxA"
                     /locus_tag="N7C74_003085"
     CDS             13292..13549
                     /gene="grxA"
                     /locus_tag="N7C74_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HGU2432908.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(13579..13956)
                     /locus_tag="N7C74_003086"
     CDS             complement(13579..13956)
                     /locus_tag="N7C74_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HGU2432909.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
BASE COUNT         3773 a         3759 c         3274 g         3248 t
ORIGIN      
        1 ttataataaa gacggacaac tcactaagtt gtccgtcttt ttttatttca tgcaaattat
       61 ttaatcatat ttattgcata taaattcact tgatggcatt gttatcccat gccgcagaca
      121 cggccaataa tcataagatt gacagacggg agttccatca tgaaaaagtt agttcttgcc
      181 gcattactgg catcctgtgc gttcggcgct tctgccgcag agaaaatcaa ttttggcgta
      241 tcagcgacct atccgccgtt cgagtcgatg gatgccaata accagatcgt cggctttgat
      301 atcgacctag caaaagcgtt atgcaaacag atgcaggcag aatgtacttt taccaatcac
      361 gcgttcgaca gcctgatccc gtcactgaaa ttcaggaaat atgacgccgt aatctccggt
      421 atggatatca caccagatcg tagcaagcag gtatcgttca ccacgcccta ttacgaaaac
      481 tcagccgtcg tgattgccaa aaaagatacc tacaaaacgt ttgccgacct gaaaggcaaa
      541 cgcattggga tggaaaacgg tactacgcac cagaaatata ttcaggatca gcacccggaa
      601 gtgaaaacgg tctcctatga cagttatcaa aatgccttta tcgatctgaa aaatagccgt
      661 attgatggcg tatttggtga cactgcggtg gttaatgaat ggctgaaaac caatccgcaa
      721 ctgggcgttg ctactgaaaa agtgaccgat ccgcaatact ttggcaccgg tctgggtatc
      781 gctgtacgtc cggataacaa agctctgctg gaaaaactga ataacgcgct ggcagcaatt
      841 aaagctgacg gtacatatca acaaatcagc gatcagtggt tcccgcaata attattcatt
      901 tgccggagtg ggcgaacacc cactccggca aaatcacttc tctacgctaa tcccaggcgc
      961 tgaaactgct ctggcggcgc atcattatct ttcatcaact gaatcagcaa cgcgatcatc
     1021 cgggactcga tcacatcatc atgaatcgga tgtagctgct gcggatcgtt ttgcagatca
     1081 aacagcaaat gaccaaactg attcaacccc ttatattttt cctgtgccgg gatgcgtaat
     1141 acctgtaacc ctttggtaaa acgaaacggt ggggccagcg ataaagcccg catctcatcc
     1201 accggatagc ggctgttcat tttgcaggac atcaacgtgt agttggcaat atcatcctca
     1261 cgccccggca gcgctgcacg catatacaca taacgcccat cagttacgtt agcatgacca
     1321 ctgaaaacac caaacatcgc cgagctgcgt actggttcat cgtgactgac cgttttcgcc
     1381 agcggttgcc cctgcacatc gggcggcact ggctgttgaa aaaagtcata caacgttggc
     1441 gcccagtcga tcatttgtac cagtgcctgt cgccgctctc cttttacacc actgcgcggg
     1501 ttccagataa acaacggatt attggcaact tcgttgtaat aaggcatctg gtttttcgcc
     1561 caccagccat gctcaccgag taaaaagccg tggtcagtgc cgacaatcag catggtatct
     1621 cgccacagat cgtgctcatc catcaggtcg agaatgcgcc ccaggttgcg atcgcacatc
     1681 gacaccagcg aacgataccg gcaacggatg tgctccacgg cctcgtcgct ttcctgcacc
     1741 cggccacgcg gccagtcata atgcggtcca tcccagttat cttcatacaa ggaaagatac
     1801 tcttccgttg tgtgaaacgg ctcgtgcgga tcaaacgttt cgatctgcaa taaccagtta
     1861 tcttccgcgt gatttttgtg gataaaggca cagcctgcat caaacacttt agtttgtgga
     1921 aaatccgctt cctgctgaat atattcccgg tttgcccagt catggcgcca cagaccagaa
     1981 acaccgccac cggtctgctt ttgcggcacc cgtagcacct cgggaatagg cggttgcgca
     2041 acgcttgcat gccagtgatc gccttcctgt ccgcgcacaa tctcccacga actgtagcga
     2101 ttatgataat tcccgccgcc atcttcccag taatgcaaat gatcgcttat caggtgggta
     2161 tagatccccg cctttttaag cagctccggc atggaatcgt caaacggttc taacggcccc
     2221 cactcccgat gcaggaagtt acatcgcccg gtatgcaact cccgccgggc aggcatacac
     2281 ggcatactgc cgacgtaact gttttcaaag gtggcggcat gagccgccag acgttggaaa
     2341 ttaggcgctt tggttaacgc atcgccataa ggtggcagat aacgtttatt cagactatcg
     2401 aacagcagaa taatcgctct cattacgccc taccttattg attaattaaa tgcggaaaat
     2461 gttgctgaac cacttcataa atttgctgac caatagtttc accgtccata atattctcta
     2521 tcggaatcaa atattgccct tgtttcgggt caagactaac cacatcgagt aattttttgg
     2581 aaatagcaaa aatatccgcc cagtcggtaa atccgccgac ttcacctgcg gcggtatgtt
     2641 ccatttcaac tttgaaatca tatttggtca gcgctttttt aacgaccgtt tgtaacataa
     2701 cgctactgcc tacaccgtaa ccacagacaa acagaatatt aattgtatct ttcattttta
     2761 aacccttaat atttaattaa cccgcgtaag gaccaagatg gaatgttgca gcgataaatt
     2821 tcagaacttc acaaatagca ggccatacgg ttgcccagtc aaatattcct gtccagccga
     2881 cgccattttc agcaagcccg gttaacggaa tagcccacac cgtaccgaag gtctgaataa
     2941 tacccagcaa gaacgtacag ataattacgg aacggtatcc gcccatgcgg tttgctaata
     3001 cgccaattgg accgccagag aaaaacagag gtacaaagcc aggcaatacc ataattgggc
     3061 tgtgaaccag taataagatc cccatcgaaa aaatggtgcc aaaagtacag aagacgaagc
     3121 ctaacgttgc ggcattgggt gaaaaaggta acagcgctgc aacatcgaca gcaggaatag
     3181 cgttaggaat aaagcgatct tgccagcctt taaacgaacc gttaatttcg cccaccatca
     3241 tgcgcacacc ctgcagcaga atgaccatat acatggagaa ctgtaagcca aggttgatga
     3301 tatagatgta ccagggcttg cctttcgcca ttaactggac attatcgata cccgttgata
     3361 gcaagaaacc accgacaaac agcgtcatca caatggcgat agccgttacg ttatggttaa
     3421 aaatcgccag ccacccggga agtttcaggt tttcagcatc gtgcttttca gggtcaccaa
     3481 atttatgggc gaatttggag aaaaaccaca atcccaacat ctggttatga gcaatggtaa
     3541 aaccggcatc tttggtaact ttggcaatgg gcttaacaat gagcgtggtg gagaacgccc
     3601 agaatacacc cgttaatatt ccggcaataa taatggatgt agtccagcta aaacccagcc
     3661 aaaagacaat caaccataat accgcctgag aatgtgaaac accggtattc cctgtcaaaa
     3721 atatcccttt ggcaccagta taccgaccaa ataataccag cagcaggttg gtaaaaaacg
     3781 ccagtacaaa tacatacccg acataaccaa aattatcccc caatgcaatt tgcgttgccg
     3841 ctttcattgc gtaggaatcc attaaatatc cgtgaacacc ataggcttta cttacggctt
     3901 ctgctaccgg tttaaatgtt gcggaaaact ggaaaccacc aaataacacc atcattaatc
     3961 ccaccatggc gctgaccatg ccggtaataa tttttattgg cgatttctta tcaagggcat
     4021 aaccaatacc caccagcaaa ccaattaaaa ttgccggttc agttaaaaac gtttgaataa
     4081 catctatgaa tttggcaaac atcgtcggaa cgccctccat acgcgtacat ttccgttgtc
     4141 aatgcatgaa ccttctgctg acgttaataa tgtctcagga aattttgtgt ttcgctaagc
     4201 aatgcctctg tatgtattcg gtaagtgcac tgtagtaatc aaaacagttc aattaaagat
     4261 cttatttgat tatttgtgat ttgatttgca aaaatagagc aatatataac cgtgatcgat
     4321 cggttattat taatcgatcg atcacgatta ttattttgga tggatgtatg ccgaggtgat
     4381 attgatttga atgatgtatt tacattttta ccagcagcgt cagcacttca tagtgcgcgg
     4441 tgtgcgggaa catatcaaaa agctgtaccc gttcgatgcg gtaacctggc agttcgcgga
     4501 tatctttcgc catggtttgg gcgttacagc tggagtagat aataaaacgc ggtgccatcg
     4561 ttgagagata atcacacagc ggtttaccaa tgccgcggcg cggcgggtta accagcacca
     4621 gctccggcac ctccccctga gcggtggcaa actgagtgga gtccagcgcc tgaaattgca
     4681 gacgcgttaa gcccagttca gcggctgact gctttgcaca ggcaatggcc tctagcgcaa
     4741 tttcgatccc ggttaactgc atgtcaggcg tcgcgcagtg taaaccaaag ccccccacac
     4801 cgcagaacag atcccacata tgtttaaccg gcagctgtcg tacccagtcg cgcgcggtag
     4861 cgtacagctg gctggcgacc gccggattag tctggaagaa actttgcgga cggatccaca
     4921 gcggcacatc gttaaaacgc tctaccagcg cctgttgttc ggtcaggtag atctccgtct
     4981 ccccttccat aatcgccata tgtaccggct gaatattgac ggtgataact tttagctgcg
     5041 gcaattgtgc ctgtaaccac ggcaatgcct tacgcagttg tgctagtttg gtttcagaac
     5101 gcagtacaaa gcgcagcatc atgccgccat cgctctggct ttcagtcagc agaatgtatt
     5161 tcagttcgcc acgcttacgc gccacgttgt agggcgttaa ccccgctcgg gcgataaacg
     5221 gttttagcgc cgcaaaaacg ggcgcaaatg aggcaggata aagcgggcag tcacaaaggt
     5281 cttccggtgt gccgtcgcga tgcagcatac cgagcagtgg tttttcaacg ctaccactca
     5341 ccaccatttt ggctttatta cgaaaccctt gttccgggcc tgacaccggc gcgcaccatt
     5401 cctcaaccgg aaagtcggcg agcagatttt taagatcggc ggttttagcg gagagttgct
     5461 ctggaatcgg ctgcgttatc cactgacagg aacgacagcg acccgcgtcg taaagtgcgc
     5521 actgcataca ttgaccttca catcatcagg ggcgacgatt atacacatta ttgcaactgg
     5581 aagaaccgcc gactggtcga gggaacgaac agcagcatca ggatgagcat atccggcagc
     5641 ttctgcagca tcaggctatg gaagatttca cgttttgatt caccgggaat gctgaacagc
     5701 tccggataac cgtaccccag cgaagccgcc cacaagtaac ttgcggcagt gatttgtgtc
     5761 agcagataca gccagcgcgc ccagcgacgc cctttcacca gtgaaaacgc acaccagatc
     5821 tcaatgaaaa ccagcaccag actgcttaaa aagaccagcg ttaaactcca ggtttgtacg
     5881 ctgcgatgaa tgaactcgcc aataccgcgc acgcccagcg tattgaaaat catcagcacg
     5941 tcgaggccac ggatcataat aatggcgagc gccgccacct gcacaagcgc aggcacattc
     6001 aggcgagcat gagatgaaga tgttttctta aaaaatccca acgtttcgtc ttccatgaaa
     6061 acaatgccgc gacatacgcg gcattatgta gccaggttgg caaattttag tgtcttcagc
     6121 cacgtcttgc acactggatg tcgcgtatcc gctgtttttc tgcgcgagcc atcagatacc
     6181 aggcgataaa tccgacaatt ccgaccgcgc cgaggatcaa tgttgctagg gcgttgattt
     6241 ccggattcac ccccatccgc acgctggaaa agaccagcat cggtaacgtg gtggctcccg
     6301 gcccggatac aaagctggcg atcaccagat catcaagcga caaagtaaaa gccagtaacc
     6361 agccagaaat gaccgcgggc atgatcatcg gtagcgtaat gacgaaaaac actttcagcg
     6421 gcgtcgcacc gagatccatc gctgcttctt ctatcgagcg atccagttcc cgaagacgcg
     6481 acgaaataac gactgccaca taagccgtac agaaagtgac atgcgccagc cagatggtaa
     6541 gcataccgcg gtccgcaggc cagccaatgg catgagcaag cgcgacgaat aataacaaca
     6601 gcgacaagcc cgtgatgaca tctggcatta ccagcggcgc ggtgatcata aaggcaaagc
     6661 catttgatcc gcgaaacctg ccaaagcgca ccagcaccac cgccgcaata gttccgagga
     6721 tcgccgccgc cgtcgccgca caggccgcaa ttgtcagact taaaccaacc gcactcatca
     6781 tcgcgtcatc gcgcaataat tcgccatacc agcgcgttga ccagccggcc cacaccgtca
     6841 ccagcttcga gctgttaaac gaatagataa ccagcatcag cattggcgcg tagagaaagg
     6901 tgaagcccaa cagcaaaatc acaatccgcc agggcgaacg aactaccggt aaattattca
     6961 tccgtgttct cccacgcttt tttgctggtg tttgtgaaac cacattatcg gcacaattag
     7021 cagcagcaac atgatgatcg ctaccgccga ggccaccggc cagtcgcggt tattgaaaaa
     7081 ctcttgccat agcacgcgcc cgatcatgat gctgtccggg ccaccgagca gttccgggat
     7141 cacaaactcg cccacagccg ggataaacac cagcatcgat ccggcaataa tcccaccttt
     7201 agtcagcggc acgatcacag taaagaacgt tttcagcggt cgtgcaccga gatccagcgc
     7261 tgcttccacc agcgaataat caatacgaat caacgcggta taaatcggca gcaccataaa
     7321 cggcacgtag gcgtaaacaa tgccaatata aactgccaga ttggtatgaa gaatggtcag
     7381 cggttgatcg ataaccccca gccacagcag aaaattattc agcacaccgt tgttttttaa
     7441 tattcccatc caggcgtaga cacgaatcag aaacgaggtc cacgacggca ggatcaccag
     7501 tagtaataag atattacggg tcgaaggctt actgtgcgcc accgcccacg ccagcggata
     7561 gccgatcagt aaacagcaaa atgtcgaaat cgccgccacc tgtaacgact ggagataagc
     7621 atcgaaatag agcggatcgc cggtcagttg caggaaatta ccgaaattaa gggtgatgga
     7681 cagttgcccg tcagcccact ccatcagttc ggtataaggt ggaatagcgc gcgccatctc
     7741 cgccaggctt attttaaaga caatcagaaa tggcagcaga aacagcaaga tcaaccagat
     7801 atacggcaac gcaatgacca gtttgcgccc atgcttcatt tgcaactgcg acaaccacag
     7861 cttaaatccg cccggcggtt tcgactgggc agcaggttca agtgtactca tctttgctcc
     7921 ttaaaccgtc agcaccacac agctgtccac ttcccagcac aaacgcactt cgtcgcccca
     7981 ggtcggtaac cctttacgat gacgatgggc gttttgtagc tgggcgctaa tcatctgccc
     8041 acttttcaga cgaacgtgat acaccgaaag atcgccgaga taggcaatgt gtatcacctc
     8101 ccccaccgcg aagttacaac cattggcggg cggctcttcg caaagcatga ttttttccgg
     8161 acgcagcgct acgtgtaccg gcacgttatc gaccactgaa gcatccgcat cgactttcag
     8221 tggatgcacc agccccggcg aataaagcac cagaccatct tcctgacgct ctttgagtac
     8281 accttcaaag acattaaccg agccaataaa ttcggcgcta tagcgggtag tcggatgctc
     8341 gtagatctct tccggttcgc caatctggac aaatttccca cgattcataa tggcgatacg
     8401 ccccgccatg gtcatcgcct cttcctgatc gtgggtgacc atcacacaag tcacaccgac
     8461 gcgttccaga atatccacca cttcaagctg catccggtca cgcagctttt tatccagcgc
     8521 gcccatcggc tcgtcgagca gtaatagttt cgggcgcttg gcaaggcttc gggccagtgc
     8581 cacacgctgt cgctgaccac cggaaagctg atgcggtttg cgtttggcga actcctgcat
     8641 atgcacaagg ccgagcatct cattgacccg gctggcaatt tccgctttcg gtagtttgtc
     8701 ctgtttcagg ccaaaagcga tgttctgttc cacggtcata tgcggaaaca gcgcgtaaga
     8761 ctgaaacatc atattgatgg ggcgcaggta aggcggcacc tgtgacaaat cgacgccatc
     8821 aagcataatc tgcccggctg aaggttgttc gaaacctgcc agcatacgca gcagcgtgga
     8881 cttgccacag ccggatgcgc ccagcagcgc aaagatttca cctttgtaga tggtcaggct
     8941 gacatcatca accgcatgct gaccatcgta ggatttggtc agattgcgga tttctaatag
     9001 cggggtcagc gccttacggg ttttcgcctg ggggcgaggg atagcgtcat tcacggctgc
     9061 tctccggcat taaatgtgtg caagcgcacc atgttggtgc gaattgccgg agggagtggc
     9121 gctcccgccc ggcatctaag gctgcggatt attttccgct cttcactttg gtccacgcgc
     9181 gggtgcgcac acggtcgatt ttcggagcct gtactttcag agtgaacaac ttcgcgcgga
     9241 catccgcagg cggataaata cccgggttat cacggacttc cgcactcacc agcggcgtgg
     9301 ctgctttatt ggcgttagca tagaacacat ggtcagagat atgtgccacc acatcaggac
     9361 gcagcaggta attcaggaac tgataggctt cgtctttatt tttggcatcc gcaggcatgg
     9421 cgaatacatc aaagaacgcc atcgcccctt cttttggaat cgagaacgag acattcacgc
     9481 cattcttcgc ttccttcgcg cggtttgacg cctgccagac atcacctgcc cagccgatag
     9541 cgacgcaaat atcgccgttt gccaggtcgt taatatattg agatgaatgg aaataacgaa
     9601 tgttcgggcg cagctttaac agcagatcgg tagccggtcc ggtgtaatca tccgctttag
     9661 tgctgttggg atctttgccg agataattca gcacggtagc aaaaacttct tctggcgcat
     9721 cgaggaaaga tacaccgcag cttttcagtt tttccagatt ttcaggcttg aggatcaagt
     9781 cccagctatc gacgggcgcg ttttcgccca gcaccgcttt gactttatcg acgttatagc
     9841 caatcccggt cgtcgcccac atatagggca tagcaaattt attgtcggga tcgtgtttgg
     9901 cgaccaactt cagcagttcc gggtcgagat tcttccactc cggcaatttg cttttatcca
     9961 gcggctggaa aactcccgct gtcagctggc gttcaagaaa gcttgctgac ggcaccacca
    10021 ggtcaaagcc ggtgctcccg gccattaatt tgccttccag tacttcgtta gagtcgaaaa
    10081 catcgtagac gactttaata ccggtttctt tttcaaagtt agccaccgtg tcgggggcga
    10141 tataatcaga ccagttataa atgtggagtg ttttttgttc agccgcgagc gtgccgacag
    10201 agacggccat cagagcaccc gcaaccagac ccgaaagcca ttttttattt aaggcggtca
    10261 tatctctttc cttctgaaag ttcgttaaca aacgaacaaa agttgtcaca ctgaaagata
    10321 tgcaataaac gtgcatattt tttatcggcg cggtgtaaga aaagagaact ctctcccggc
    10381 cagcactgct cgctatataa aagcatcgca agaataactt tagccagggt ttgagactat
    10441 agctcggatt aaaaaacggt atttaacaaa tttttatgcg tatttgctct atgtgataaa
    10501 gccaaatcga cacaagagaa gtgaaataat ctttaagaaa aggttaaaaa cagcagataa
    10561 ttttgcagcg cgcagccact gcaccagcga ctgcgtgctc gaaaggagtt atcagtggag
    10621 gaaatagttt tcggtaacgt tgttttgccc ttcatcgtca gtcggtaaca gcagttgatg
    10681 ggcattagct tcaagaatga ccatcgaaat ctgctcttcg ctctgacgca cgaaccatgc
    10741 aaactgctca taggttacgc cctgcataac ggataaagac tggcaaacca ccagctttgg
    10801 cagattatca tcctgcatat caagaaatgc tttcacggtc agcgaactgg cattgatggc
    10861 agataaatcc gccgccagcg gcaatacggc tgaaggtcgg acttccgcca tggcagaaaa
    10921 caggatcgtg ttatcgatca gatcgatttt ggcatcaaag acaccgtcga aattctgcat
    10981 atggggcaga tgcagagcct gacagttatc acattcaaaa aaactcatcc ccaggtcatc
    11041 gagccattga cgcagcgtat ccagaccagg aacgaccagc gatgtcataa ttttgtacaa
    11101 cctctttcga taaaaagacc ggcacagctt acgcaaaaag agcaggcaaa accatgatca
    11161 gtaatgtgat tgcgactaac cacccgtttt caggcaatat tctgtcgtag cgtggcgttc
    11221 gatccagcgg atcattttac ccgcgatgtc gataccggtg gttttttcta ttccttccag
    11281 ccccggcgac gcattcactt ccatcaccaa cggcccgcga ttagcacgca gaatatccac
    11341 accagcaacg tccagcgcca tcgttcgcgc ggcttttatc gcgatttcac gctcctgtgg
    11401 tgtgatactg gcgacgcttg ccgcgccgcc acgatgcaaa ttggaacgaa aatcgccctc
    11461 tttcgcccgc cgttcaatcg cagcgaccac ttcatcgcca acaaccagac agcggatatc
    11521 gcacccttgc gcctctttga tatattcctg caccagaata tgcgcgttca gaccgcggaa
    11581 agcgtcaatc acgctttccg ccgcctgacg cgtctccgcc agcacgacgc caattccctg
    11641 cgtgccttca accaacttga ccaccagcgg cgcaccaccg accatgtcga ttaaatcgct
    11701 ggtatcatcc ggcgaatgcg caatgcccgt gacaggcagg tcgatgccct gacgcgccag
    11761 cagttgcatg gaacgcaatt tgtcacgcgc ccgggcaatg gcgaccgact cattgagcgg
    11821 atagctcccc agcatctcga actggcgcag tgccgccgtc ccataaaagg taatggcggt
    11881 gccaatacgc gggatcactg cgtcaaaatg gggtaacttg cggcctttgt agtgaataga
    11941 agacgccgca ggatttatgt tcatgtagca agaaagcgga tcaagaattt caaccaggtg
    12001 accgcgctgt atagcggctt cacgcagccg cttacacgaa tagagcgttc catcccggga
    12061 caatatggca attttcaccc tgcacctctc tgtcagacct ggtcaaaagc ccgcgtatca
    12121 tacatcggcg tggcggtttt agcgcgtcgc ccaaccctgt ttgtgcaaat aatccagaat
    12181 aaatgggcgg ctttctttaa tgattgttcg gcggatatga tcgctccagg tatcccggcg
    12241 attattgctg ccacgggtga ggtaatattc agccagttgt tcgtcatact gcgccagtgc
    12301 gtctttatcc agcggttgat agctgttttc atgcaccaaa atggaggacg gtaaacgcgg
    12361 cttaagatcc ggattatccg caggccagcc aaggcacagc ccaaacagcg gcagaacatg
    12421 ctgcggtaat ttcagcagtt tcgtcaccgc ttcaatatta ttgcgcaggc cgccgatata
    12481 taccccgccc aatcccagcg attccgctgc ggttaatgca ttctgcgcca tcattgccgt
    12541 atcaacgaca ccgagcagca attgttccgc caggccaagc tgagcatccg gacagatctg
    12601 taaatggcgg ttaaagtcgg cacagaacac ccagaactcc gccgcttgcg ctacgtgttt
    12661 ttgcccgccg gtcagcgtca ccagttcttc acgtaacgct ttgtcggtaa tgcgaataat
    12721 gctactgcac tgcaaaaaac tggaactgga cgtcgcacgg gcgctgttaa taatcgcctc
    12781 acgctgcgcc tcggaaatgg gttcatcagt gaaatggcga atggagcgat ggccacaagt
    12841 aagttcaatg gttggcgtca ttatcttttt ctctttctga acgtgaatac tgcggtggac
    12901 ggttcatcag ctgtggggca agacgttttg ccacctgaag aataaccacc accgcagcgg
    12961 gaagcatgag caaaacaccg agaaaaatca tcagaatctg cacttctggc cgagaaaatg
    13021 gctcaggcag cgacagggag tcgcttaccg acagcagcgc caccgccagc aacatcattc
    13081 cgataaattc cagtatcaac acgcttttag gcaatttacc gatcgcgcgc atacgcttcc
    13141 ctctgcaaag tgaaccttca gtctaaaact tttcactgta ttgtgtttaa cagttatagc
    13201 ttttagcaat taatgcaaca ggttaaacct actttcagcg aatacatttt agcgtgatca
    13261 ttacaggcat aaatctatga ggagagaaat aatgcaaacc gttatttttg gtcgtccggg
    13321 ttgcccttac tgtgtgcgtg caaaagatct ggctgagaaa ttgagcaatg aacgcgatga
    13381 ttttcagtat cagtatgtag atattcgtgc ggaagggatc actaaagaag atctacaaca
    13441 aaaggcaggt aaacccgtag aaaccgtgcc gcagattttt gtcgatcagc aacatatcgg
    13501 cggctatacc gattttgctg catgggtgaa agagaatctg gacgcctgat cgtctgacaa
    13561 gccctcgcgt tgagggcttt actgattttt tctgtgctgt ggtttaaaca agctactgat
    13621 aaataagaaa cacagtgccc ccagcgcaca ccagaacacc gcgcttagta accatgccag
    13681 ctcttgccag aatgagcggg tcggtgaaaa aaacagccgc ataataagca ttgaacaggg
    13741 tgccgccagc attgcgccaa atagaggttt caggacttct ctacgctgtg aaaagaaact
    13801 ggcaactgct ccaggaagaa tgaaaaacag caagccgatt tcaggatgcc cggcagcccg
    13861 aaaagcgcct ttcatgtgcg tcgccagaaa aaggcacacc acaatgaaga ggacaaaaca
    13921 gcagattgcc cccgcccaac gttgtttatg tttcactcgt tcctcctgac actgcgtcta
    13981 tcgaacacat ttttcgccag tgtggcgttc agtaagataa agccgcttcg cattccatgc
    14041 taatataggc caac
//
LOCUS       DBFIFM010000121        13940 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.121, whole genome shotgun sequence.
ACCESSION   DBFIFM010000121 DBFIFM010000000
VERSION     DBFIFM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.121"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            137..646
                     /gene="ybgA"
                     /locus_tag="N7C74_003087"
     CDS             137..646
                     /gene="ybgA"
                     /locus_tag="N7C74_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HGU2432910.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIESTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPYAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            643..2061
                     /gene="phrB"
                     /locus_tag="N7C74_003088"
     CDS             643..2061
                     /gene="phrB"
                     /locus_tag="N7C74_003088"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HGU2432911.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARLLALYIATPRQWAA
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFAASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVQVERTLRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRNVPGKSVHEPWKWAEKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(2211..3692)
                     /gene="dtpD"
                     /locus_tag="N7C74_003089"
     CDS             complement(2211..3692)
                     /gene="dtpD"
                     /locus_tag="N7C74_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HGU2432912.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELRLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            3963..4706
                     /gene="ybgI"
                     /locus_tag="N7C74_003090"
     CDS             3963..4706
                     /gene="ybgI"
                     /locus_tag="N7C74_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HGU2432913.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            4729..5385
                     /gene="pxpB"
                     /locus_tag="N7C74_003091"
     CDS             4729..5385
                     /gene="pxpB"
                     /locus_tag="N7C74_003091"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HGU2432914.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            5379..6311
                     /gene="pxpC"
                     /locus_tag="N7C74_003092"
     CDS             5379..6311
                     /gene="pxpC"
                     /locus_tag="N7C74_003092"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HGU2432915.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKSRQDQQRYFEQLAWRLHNEN"
     gene            6301..7035
                     /gene="pxpA"
                     /locus_tag="N7C74_003093"
     CDS             6301..7035
                     /gene="pxpA"
                     /locus_tag="N7C74_003093"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HGU2432916.1"
                     /translation="MKIDLNADLGEGCTSDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRKNFGRSAMQLPPETVYAQTLYQIGALATITRAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQSGALIEDEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSAFAEKGIVVAA"
     gene            7071..7861
                     /gene="nei"
                     /locus_tag="N7C74_003094"
                     /pseudo
     CDS             7071..7861
                     /gene="nei"
                     /locus_tag="N7C74_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
     gene            complement(7858..8904)
                     /gene="abrB"
                     /locus_tag="N7C74_003095"
     CDS             complement(7858..8904)
                     /gene="abrB"
                     /locus_tag="N7C74_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HGU2432917.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLGLHLPAALLLGPMIAGIIFSMRG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLEVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQHIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRTA"
     gene            complement(9053..10114)
                     /gene="ybgO"
                     /locus_tag="N7C74_003096"
     CDS             complement(9053..10114)
                     /gene="ybgO"
                     /locus_tag="N7C74_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2432918.1"
                     /translation="MRFAKGVLLAICLIFLPLKAALALNCYFGTANGAVEKSEAIMPF
                     AAPANSKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGIQDPYYQLGVTY
                     EGVDYDASLGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKLCSGDPTVMHKSRTFVA
                     RMRLYVKVRAMPPHDYQSKLSDYIVVQFDGAGSVNEDPTAKNLKYHITGLENIRVLDC
                     SVNFAISPETQVVDFGRFNVLDIRRHTMSQQFKITTTKSQNDQCTDGFKVSSSFYTDE
                     TLIDEDKSLLIGNGLKLRLLDENASPYTFNKYSEYADFTSDLLVYEKTYTAELSSTPG
                     TPIDVGPFDTVVLFKINYN"
     gene            complement(10111..10842)
                     /locus_tag="N7C74_003097"
     CDS             complement(10111..10842)
                     /locus_tag="N7C74_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU2432919.1"
                     /translation="MTFVKGFPLILLVASMCSHGAVQPDRTRIIFNSKDKATSLRVEN
                     RSDKLPYLAYSWIENEKGEKSDEFLVALPPIQRLEPKATSQVRIMKQAATAKLPTDRE
                     SLFYYNLREIPPVPEGSEGHAILQVAVQSRIKLFWRPAALRKKMGDHVEQQLQVSQQN
                     NQLTLKTPTGYYLTIAYLGRDEKGVLPGFKSTMVAPFSSVTTSTGSYSGKQFYLGYMD
                     DYGALRMNTLSCQRQCRLQPVENKK"
     gene            complement(10857..13316)
                     /locus_tag="N7C74_003098"
     CDS             complement(10857..13316)
                     /locus_tag="N7C74_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU2432920.1"
                     /translation="MDAVNIYRLSVLSCLAMVTPPALTAEFNLNVLDKSIRDSVDISL
                     LNQKGVVAPGDYFVSVTVNNNKISNGQQIRWQKSGDKIIPCINESLIELFGLKSDFRK
                     KLPAIKECVDFSVFPEIIFTFDQANQQLNITIPQAWLAWHSENWTPPSTWNNGIPGFL
                     MDYNLFASTYRPQSGSSSNNLNAYGTTGLNAGAWRLRSDYQLSQSDSGDNREQSGAIS
                     RTYLFRPLPQIGSRLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISHSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVSEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGRPRSSMSHHTEDETFISHEVSWGMLSNTSLYGGMLLAGDD
                     YRSGALGIGQNMLWMGALSFDVTWADSHFDTQQDEQGYSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYIDHKYNDADAQDEKQTISLSFGQPITLLNLNLYANILHQSWWN
                     ADTSTTANITVGFNVDIGDWKDISVSTSFNTTHYEDKDRDNQIYFSISLPIGESGRLG
                     YDMQNNSNTTTHRMSWNDTLDERNSWGMSAGIQSDRPDNGAQVSGNYQHLSSAGEWDL
                     TGTYAANDYTSASASWSGSFTATQHGAAFHRRSSTNEPRLMVSTDGVGDIPIQGNIDY
                     TNRFGIAVVPFVSSYQPTTVAVNMNDLPDGVTVSENVVKETWTEGAIGFKSLASRAGK
                     DLNVIISDANGHFPPLGADVRQAEGGVSVGMVGENGHAWLSGVDENQQFTVHWGDQKT
                     CAIHLPEHLEDVTKRLILPCH"
     gene            complement(13366..13932)
                     /locus_tag="N7C74_003099"
     CDS             complement(13366..13932)
                     /locus_tag="N7C74_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2432921.1"
                     /translation="MFKGQKTLAALAISLLFASPVFAADEGSGEIHFKGEVIEAPCEI
                     HPDDLDMNIDLGEVTTSHINREHHSEKKPVNIRLINCDIAGWDDGKGGVVSKVGVTFD
                     STAKTTGATPLLSNISAGEATGVGVRLMNKDESFVTLGTEAPTIDLLASSTEQTLNFF
                     AWMEQMDNAVSVTAGAVTANATYVLDYQ"
BASE COUNT         3377 a         3453 c         3795 g         3315 t
ORIGIN      
        1 attcgctgct ttgatgatga tgtaaaatga ttgtttttga actggatata acagcttggt
       61 agggaagaaa gcataacccc acaaaattca tttaccatcg tctacactta ctcctgtaaa
      121 ccgttcagga gcagtaatga atcagcaacg atttgatgac agcactctaa tccgtatctt
      181 cgcgcttcat gagttacatc gactgaaaga acatggctta acgcgcgggg cgcttctcga
      241 ttatcacagc cgctataaac tcgtatttct ggctcattcc cagccggaat accgcaaact
      301 tggcccgttc gtggctgata ttcaccagtg gcaaaatctg gatgattttt acaaccagta
      361 ccgccaacgc gtaattgttt tgctttctca tcccgccaac ccgcgcgatc acaccaacgt
      421 tttgatgcac gttcagggtt attttcgccc gcatattgag tccacagaac gccagcagct
      481 ggctgcgctt atcgacagtt atcgccgtgg cgagcaaccg cttcttgcgc cgctgatgcg
      541 tatcaaacac tatatggcgc tttatcctta cgcctggctt tcagggcagc gttatttcga
      601 actttggccg cgtgtgatta acttgcgcca ttcaggagtt ttatgactac ccatctggtc
      661 tggtttcgcc aggatttacg tctgcatgat aatctcgcgc tggctgccgc ctgccgcaat
      721 tcatctgcac gcctgctggc gttgtatatc gctacaccac gccagtgggc ggcgcataat
      781 atgtcgccgc gtcaggctga acttatcaat gctcaactga acgggctaca aatcgcgctg
      841 gcggaaaaag gcattccttt attgttccgt gaagtggatg actttgccgc cagcgtcgaa
      901 atagttaaac aggtgtgcgc ggaaaacagc gttactcatc ttttttataa ctatcagtat
      961 gaggtgaatg agcgggcgcg ggatgtgcag gttgagagaa ctctgcgtaa cgtggtgtgt
     1021 gaaggatttg atgacagcgt gatccttccg cctggcgcgg tgatgaccgg caatcatgag
     1081 atgtacaaag tctttacgcc ttttaagaat gcctggctga aacggctgcg ggaagggatg
     1141 ccggagtgcg tcgctgcacc aaaagttcgt agtagcggat cgataaagcc cgcgccatcc
     1201 attacgctga attatcctcg tcagtctttc gatactgcgc atttcccggt ggaagaaaaa
     1261 gcggcgattg cgcaattacg ccagttttgc cagaacggtg ccggagaata tgagcaacaa
     1321 cgagattttc cggcagtgga aggcaccagt cgtttgtccg ccagcctggc aacgggcggg
     1381 ttatcgcctc gccagtgttt gcatcgcttg ttggcggaac agccgcaggc gctggacggt
     1441 ggggccggta gtgtctggct taatgagctg atctggcgcg agttctaccg tcatctgatg
     1501 acgtattacc cctcgttgtg taaacattgt ccgtttattg cctggacgga tcgtgtgcag
     1561 tggcagagca atcccgcaca tttacaggcc tggcaggaag gcaaaacggg atacccgatt
     1621 gtcgatgctg ccatgcgtca gcttaacagc actggctgga tgcataacag gctacggatg
     1681 attacagcca gttttctggt gaaagatttg ttgatcgact ggcgcgaagg cgagcgatat
     1741 ttcatgtcgc agctgattga tggtgatttg gcagccaata acggtggctg gcagtgggcg
     1801 gcttctacgg gtactgatgc agcgccgtat tttcgtattt tcaacccgac aacccagggc
     1861 gagaaatttg accgcgaagg cgagtttatc cgccagtggc taccggaact gcgcaatgta
     1921 ccggggaaat cggtgcatga gccgtggaag tgggctgaga aagcaggtgt gaagctggat
     1981 tatccgcaac cgatagtcga gcacaaagag gcgagagtac aaacgttggc agcgtatgag
     2041 gcggcgcgga aggggaaata acagacgcgt caggcaatcg agcccagatg ccggatgcgg
     2101 cgtgaacgcc ttatccagcc tacaaatttg tgcaaattca ataaattgca ggaaatacgt
     2161 aggcctgata agacgcgtca agcgtcgcat caggcatcgg tgctggccta ttaagactcc
     2221 agcgccagcg cgcggtttct gaatttcagc gcctgataca gccaaatcat cagtaccact
     2281 cctacacacg ccagtgcgcc ccaggtaatt tgatcaaata cttcaatata tgcattgatg
     2341 gagtagttga tcgccccgga agcatcaaac gaagcctgcg atgtctgatc ggcaatcacg
     2401 cccgccagat agttcgcaat cgcgccggaa agcagcatat aaatgccggt taatacgccg
     2461 gtcacaccgg ggatttcaat gcgcgtaatc tgcgacatag caaccgggtc gataaacagt
     2521 tccgcaaagc ccatcaccgc caggcctaat accatcagtg gcagagaaga gtgaccatac
     2581 attgcggacc agcgggcgct taaggtcaga atgcagaatc cggcgctcat caggccaagg
     2641 ccaagagcaa attttcccca gatgcgcacg gtacgattac ccgcgacact ctcttttacc
     2701 acccacgcca ggaacacacc gcacagcata actgcgaagg cattaatcga ctggaacatc
     2761 gcggtcggaa cggtataacc aaacatatcg cgattaacga agcggtcgat ataaaggcta
     2821 atcgagctac cgccctgttg tgcgaaggcc cagaacaaca tactgaagaa ggtgagcgtc
     2881 acaatcagcc tcagctcctt ccgctgtttc tggttttctg ctttgcgata aatttttgcc
     2941 agtacaccca ggccaatgat agtcgcgaca atcaaggcgt ataccgacca ttctttccag
     3001 aacagtacgg taatcagcgc aggcgttgcc accagcagaa ccagcagcca tccccagttc
     3061 ggcagcagaa agtttgtcgc acgcagtact tttttgttaa cgccgcgggt atgtgtgaaa
     3121 tgacgattgc cacataagaa aataaccaga cccgcgatca tacccaccgc cgccaggcca
     3181 aagcccatcg cccaactgta ctcttcctgg gcgtagccac aggcaatagg tgcgataata
     3241 gaccccacgt tacccgctgc atacatcagc gagaaaccgc catcacgacg cggatcggtt
     3301 ggctcataca gctcgccgag caggcaactg acattggatt taaacagacc atagccacaa
     3361 acgattatcg ccagggacag atagaggaat gacggatgga tctcacttgc acccaacacc
     3421 acatgaccga tcgccatcaa taacgccccc agcatcaccg ccatgcgatt gccgagaact
     3481 ttatccgcca aaaagccacc gaggattggc gtgacataca ccagcgaaca gtaggcgcta
     3541 aataactcgt aggcgtgagt atcgttgtat tttagttgat tggtgagata gagaatcagc
     3601 agggcacgca tgccgtaaaa gctaaaatat tcccagattt gcagcgcaac gacatagtaa
     3661 atagcgcgcg gttgtgatgc gtgtttattc ataataattc cacaattgac ggcaacgtca
     3721 cgtaatagtt gcgcggcgtg cttgtgtttc cttaaagtta aaactttgat acagatctga
     3781 ttatttatgc aatatgctgt ctgattgcat aaatatacat tagctgaagc gtgatgatat
     3841 aaacaatttg cgtcttttgg cgtcgggttt gtttcttaat atgtcaaaag attggctatc
     3901 gacgaacagg cgaggtaacg ctatgttaac ggtgtagcta tcagaatacg gaaggcgcga
     3961 agatgaaaaa caccgaactg gaacaactga ttaacgaaaa actgaacagc gcggcgatta
     4021 gtgattacgc gccgaatggt ttgcaggtgg aaggcaaaga gacggtgcaa aaaattgtta
     4081 ccggtgtcac tgccagccag gctttgctcg atgaggcagt gcgtctgggc gcggatgcgg
     4141 tcattgtgca tcatggctac ttctggaaag gcgaatctcc ggtcattcgc ggcatgaagc
     4201 gtaaccgttt aaaaacgttg ctggcgaatg atatcaacct gtatggctgg catttgccgc
     4261 ttgacgcaca tcctgagctg ggcaataacg cacagctggc ggcgttactg gggatcacgg
     4321 tcatgggcga aattgagccg ttggtgccgt ggggcgaact gaccatgcct gtgccgggac
     4381 tggagctggc ttcctggatt gaagcgcgtc tgggacgtaa gccgttatgg tgtggcgata
     4441 ccggacctga ggtggtccag cgcgtcgcct ggtgcacggg cggcgggcaa agttttatcg
     4501 atagcgccgc gcgttttggc gtggatgctt ttattactgg cgaagtttct gaacagacca
     4561 ttcattcagc ccgcgagcag ggattgcatt tttatgctgc aggtcaccat gccactgaac
     4621 gtggtggtat tcgcgcattg agcgagtggc tgaatgaaaa taccgatctt gatgtgacct
     4681 ttattgatat tcctaatcct gcataacgaa taatcagagg gatcgaaagt gcaacgagcg
     4741 cgttgttatc tgataggtga aacggcggta gtgctggaac tggaaccgcc ggtgacgctg
     4801 gctagccaga aacggatctg gcgactggcg cagcgtctgg tggatatgcc gaatgtggtt
     4861 gaagccattc ccggcatgaa caatatcacg gtgattttgc gtaatcctga gtcgctggcg
     4921 ctggatgcca tagagcgttt gcaacgctgg tgggaggaga gcgaggccct ggaacctgag
     4981 tctcgcttta ttgaaattcc ggtggtttac ggtggtgcag gcggaccgga tttggcggtg
     5041 gtcgcggcgc attgcgggtt gagcgaaaaa caggttgttg aattgcactc ctccgtggaa
     5101 tacgtggtct ggtttttagg ttttcaaccg ggcttcccgt atctcgggag tttgccggaa
     5161 caactacaca cgccacggcg cgctgaaccg cgcttgctcg ttccggcagg ttctgtcggg
     5221 atcggcgggc cgcagactgg tgtttatccg ctggcaacgc cgggtggctg gcagttgatt
     5281 ggtcatacct cactcagcct gtttgatccg gcgcgtgacg aacccatctt attacgtccg
     5341 ggagacagcg tgcgctttgt accgcaaaag gagggagtat gctgaagatt attcgtgcgg
     5401 gcatgtatac cactgtgcaa gatggcggtc gtcacggttt tcgccagtcg ggtatcagcc
     5461 actgcggcgc actggatatg cctgcgttac gcattgctaa cctgctggtg ggtaatgatg
     5521 ccaatgcccc cgcgctggag atcacgctcg gtcagttaac ggttgagttc gaaactgatg
     5581 ggtggtttgc tctgacgggt gccggttgcg aagcgcggct ggatgataat gccgtctgga
     5641 ccggctggcg attgccgatg aaagcaggcc agcgtttaac gcttaaacgc ccgcagcacg
     5701 ggatgcgcag ttatctggcg gtcgcgggtg gtattgatgt tccgccggta atggggtcat
     5761 gcagcaccga tctcaaagtg gggattggcg ggctggaagg ccgtttactg aaggatggtg
     5821 accgactccc gattggcaaa gcgaagcgtg attttatgga agcgcagggc gttaaacagc
     5881 tgctgtgggg caaccgcatt cgcgccttgc cggggccgga atatcatgag ttcgatcgcg
     5941 cctcgcagga tgcattctgg cgttcgccct ggcagcttag ctcgcaaagt aaccgcatgg
     6001 gctatcgctt acaggggcaa attttaaaac gcaccaccga tcgcgaactg ttatctcacg
     6061 gtttgttacc gggcgtggtg caggtgccgc ataacgggca gcccattgtg ttgatgaacg
     6121 acgcacagac caccggtggt tacccgcgta ttgcctgtat cattgaggct gatatgtacc
     6181 atctggcgca aattccgctc ggtcagccga ttcattttgt ccagtgttca ctggaagagg
     6241 cactgaaatc gcggcaagat cagcaacgtt atttcgaaca attagcgtgg cggctgcaca
     6301 atgaaaattg acctgaacgc cgatctgggc gaaggctgca ccagcgacgc agagctatta
     6361 acgctggttt cctctgccaa tattgcctgt ggatttcatg caggcgatgc gcaaaccatg
     6421 caggcttgcg tgcgtgaagc aataaaaaat ggtgtcgcga ttggcgctca tccgagtttt
     6481 cccgacagga aaaattttgg tcgcagcgct atgcagctgc cgccagaaac cgtttacgcc
     6541 cagacgctgt atcaaattgg cgcgctggca acgattaccc gtgcgcaagg tggtgtaatg
     6601 cgtcatgtca aaccgcacgg catgttatac aaccaggcgg cgaaagaggc acaactggca
     6661 gacgccatcg ccagagcggt atacgcttgc gatccagcat tggttctcgt tgggctggca
     6721 ggaagcgagc tgattcgtgc aggcaagcaa tatggcctga caacgcgcga ggaagtgttt
     6781 gccgatcgcg gttatcaggc tgacggctcg ctggtgccgc gaagccagtc aggcgcgctg
     6841 attgaagacg aagaacaggc gcttgcgcaa acgttggaga tggtgcaaca cggcagagtc
     6901 aaaagtatca ccggcgaatg ggcaacggtc acggcgcaaa cggtctgcct gcatggcgac
     6961 ggcgagcacg cactggcgtt cgcccgccga ctacgctctg catttgccga aaaggggatt
     7021 gttgtcgcag cataaccccg attaataaag aatgaaaaaa ggatatcacc atgcctgaag
     7081 gcccggagat ccgccgtgca gcggataacc tggaggcggc gatcaaaggc aaaccactaa
     7141 ctgatgtctg gtttgccttc ccgcagttaa aaacttatca atcacaactt atcggtcaac
     7201 acgttaccca tgtggaaacg cgtggtaagg cgttgttaac tcatttttcc aacgacttaa
     7261 cgctctacag ccataatcag ctttatggcg tctggcgcgt ggttgatacc ggcgaagagt
     7321 cgcagaccac gcgagtattg cgggtaaaac tgcaaacggc tgacaaaacc attctgcttt
     7381 atagcgcctc ggatattgag atgttgaccc cggaacaact gaccacgcat ccgtttttac
     7441 aacgcgttgg tcccgatgtg ctggatccga atctgacgcc ggaggtggtg aaagaacggt
     7501 tattgtcgcc gcgctttcgt aaccgtcagt ttgctggatt actgctcgat caggcgtttc
     7561 tggctgggct tggcaattat ttgcgtgtgg agatcctctg gcaggttggg ttgactggaa
     7621 atcataaagc gaagacctca atgcggcgca actggatgca ctcgcacacg cgttactgga
     7681 tattcctcgg ttttcttacg ctacgcgggg gcaggtggat gagaataagc atcatggggc
     7741 gctgtttcgc tttaaggttt ttcatcgtga tggcgaactg tgcgaacgct gtggcggcat
     7801 cattgagaaa accacgctgt catctcgccc gttttactgg tgtccgggct gccagcacta
     7861 ggccgtacgt ttcggcgcat aggttgaaat aaaccgcgca atggcaggcc ctgtcagcaa
     7921 aatactgaac aggcgcaggg tttgcatcgc cataatgagc gccatatcgg cattgctccc
     7981 tgcggcgatg accgccacgg tatcaagccc gccggggctg gtggcgaggt aggcagtcat
     8041 aaaatcaatg tgcataaacc gggtcagtcc ccacgccata cccgcacaaa tagccagcag
     8101 agcaaaaatc gacagcagga tttgtggtag cgggcgtagt gcccgcagta agatttgttt
     8161 atcgaaacca aggccaatcc gccagccaat tgccatatac gccatcgcca gtagccattc
     8221 cggtagttcg atggtgatga gctgaccaga ctggagcact gcgcccgcca gcatcgggat
     8281 gagcatcgtg ccggaaggaa gacgcagcag gcatcccact gtaccggcaa cgaccgcgag
     8341 caaaatagta agcaggagat taatgctcac tggcgggaac cagacgatat gctggttaac
     8401 cgcttcagcg ttatcgccca gcatcattcg cgtgaccaga actgcggccc ccgcgacaaa
     8461 cagcactcgc agatactgca taaacgccac caggcggata tctgcgccgt aatcctgcgc
     8521 catcgcgacc attgccgccg ccccgccagg cgaggagccc caggcaccag tatttccggg
     8581 cagtgagcta tagcgcacca gtagccatcc cacgatagcg ctggaaagca acgtcaccag
     8641 taaaatagct aacacaatcg gccagttaac ctccagcgtg gtcaggatcg aaccggtcaa
     8701 gttttgcgca atcatgcagc caagaatggc ctgcgcggcg agaaaagcgg agcgggggag
     8761 ttgcagggta accccgcgca tactgaagat gatcccggcg atcatcggtc caagtaagag
     8821 tgccgccggg agatgtaatc cgaggaaacc aatagaaagg agaagtgata gcacacataa
     8881 cattccccac tgcaaaactg gcatcctttg ctccctcatt attttctctt tggtgtatcg
     8941 atacagcata aagaatgccg ggcatggaaa aaataacggt aatcaataaa agccggaaaa
     9001 ttggcaatta accgaagaag ccctctccgg ctggaaaggg caataggcta aatcagttgt
     9061 agttaatttt gaaaagtacc actgtatcaa agggaccaac gtcgatgggc gtacccggcg
     9121 tggatgacaa ttctgccgtg taggtttttt catacactaa caggtcgctg gtaaaatcgg
     9181 catactcact gtatttgttg aaagtatagg gtgaggcatt ttcatccaac aatcgaagct
     9241 tcaagccatt gccaatgagc agtgatttat cttcgtcgat aagggtttca tcggtataaa
     9301 acgaagagct gactttaaaa ccgtcggtac actggtcgtt ttgtgatttg gttgtagtta
     9361 ttttaaattg ctgcgacatc gtatggcgac gaatatccag cacgttaaat cggccaaaat
     9421 caacgacctg ggtttcaggt gagatagcaa agttgacgct gcaatccagc acgcggatat
     9481 tttccagacc ggtaatgtga tatttcagat tcttcgcggt agggtcttcg ttcacgctgc
     9541 ctgcgccatc aaactgtacg acgatgtaat cgctgagttt gctctgataa tcatggggcg
     9601 gcatggcgcg aactttaacg taaagccgca tacgcgccac aaacgtccgc gatttgtgca
     9661 taacggttgg atcgccggag caaagtttat tttgccagcc catcgcaatg atctgctccg
     9721 gggtgtaaat atcaatattc ttactgtcga tgcactgatt cgtgtcgatg cggcttttcc
     9781 caaggcttgc gtcatagtcc acgccttcat aggttacgcc caattgataa tagggatcct
     9841 gaataccggg gtaagggttt acccaggcgt agacgtgctc gctttcaaaa ttaccgtttg
     9901 tgttgttgtc acagtagacc ggaattttta tatcgtcaga ttcccagatt ttatcgccgg
     9961 gtttggaatt ggccggagcg gcgaagggca taatagcttc cgatttttct acagcgccgt
    10021 ttgcggtacc aaaatagcag ttcagcgcca gcgccgcttt tagcggcaaa aaaatcagac
    10081 agatggcgag caacacgcct ttggcgaatc tcatttttta ttctccaccg gctgcaggcg
    10141 gcattgccgc tggcaggaga gcgtgttcat acgcaatgcg ccgtagtcgt ccatgtagcc
    10201 aagataaaac tgcttgccgc tatagctgcc cgtgctggtg gtgaccgaac tgaacggtgc
    10261 gaccatcgtg cttttaaagc caggcagcac gcctttctca tcccgcccaa gataggcaat
    10321 agtcagatag tatccggtcg gggttttcag ggtaagctgg ttattctgct ggctaacctg
    10381 tagctgttgc tcaacatgat cgcccatttt tttgcgtaat gcggcgggtc gccagaatag
    10441 cttaatgcga ctttggacgg cgacttgcag gatcgcatgg ccctcgctgc cttccgggac
    10501 aggcggaatt tcacgcaggt tatagtaaaa gagagattcg cgatcggtgg gtaatttcgc
    10561 ggtggcggct tgtttcatga tccgaacctg cgaagtcgct tttggctcca gtcgctggat
    10621 aggcggcagg gcgacgagaa attcatcgct tttctctccc ttttcatttt ctatccatga
    10681 ataggcgagg taggggagtt tatcgctgcg gttttccacg cgcaggctgg ttgctttatc
    10741 tttgctatta aagatgatgc gcgtgcggtc aggctgcact gcgccgtggc tgcacatact
    10801 ggcaaccaga agaatcaaag ggaatccttt aacaaatgtc atcgtctttt ccttaattaa
    10861 tgacagggta aaatcaggcg cttagtcaca tcttccagat gttccggcag atgaatggca
    10921 cacgttttct ggtcgcccca gtgcacggta aattgttgat tttcatcgac cccactcagc
    10981 catgcgtgac cgttttcgcc aaccatcccg acgctgacac cgccttcagc ctggcgaaca
    11041 tcggcaccga gcggcgggaa atgcccgtta gcatcgctga taatgacgtt gagatctttc
    11101 ccggcgcggg acgccagcga tttaaagccg atggcgcctt cggtccaggt ttctttcacc
    11161 acgttttcgg aaacggtaac gccgtcaggc aagtcgttca tattgaccgc caccgtcgta
    11221 ggctggtagc tggaaacaaa cggtaccacg gcaataccaa agcggttggt gtagtcaata
    11281 ttgccctgaa tcggaatatc gcccacgccg tcggtgctga ccatcaggcg tggttcattg
    11341 gtggaactgc ggcgatggaa tgccgcgcca tgttgagtcg cggtaaacga gccgctccaa
    11401 ctggcactgg cggaagtgta atcattggcg gcataggttc cggtcagatc ccattccccc
    11461 gccgaactga ggtgttggta attgccgctg acctgcgcac cgttatccgg gcgatcggat
    11521 tgtattccag ccgacattcc ccaactgttt cgttcatcca gggtatcgtt ccacgacatg
    11581 cggtgggtgg tagtattact gttgttctgc atgtcataac ccagtcgacc actttcccca
    11641 atcggtaagg agatcgaaaa gtaaatctgg ttgtcgcgat ctttatcttc ataatgggtg
    11701 gtgttgaacg aggtcgagac agaaatatct ttccagtcgc caatgtcgac gttaaaaccg
    11761 acggtaatgt tggccgtggt ggaggtatcc gcattccacc agctctgatg caggatgttg
    11821 gcgtagagat taagattcag cagagtaata ggctgaccga aggagaggct gattgtttgc
    11881 ttttcatcct gcgcgtcggc gtcattgtat ttgtgatcaa tgtagttagc gtagctgtga
    11941 aaatggcgat cggagaagcg ataggctgcc aatgagatgg tgctgttggt cgcatcaacc
    12001 tgtttgctgt agttaaagcg gtagctgtaa ccctgctcat cctgttgggt atcaaaatgg
    12061 ctgtcggccc aggtcacgtc aaacgacagc gcccccatcc agagcatatt ttgcccaata
    12121 cccagcgcgc cggaacgata gtcatcgcct gcgagaagca tgccgccata tagtgaagtg
    12181 ttggagagca ttccccagga gacttcatgg ctaatgaagg tttcatcctc ggtgtggtgc
    12241 gacatagagg aacgcgggcg accggcagcc agtttatagc gcacctggcc ctgacgcgtc
    12301 aggaacgggg tagaggccgc tgaaacctgg aagttattca cccgtccatc ttcttcgctc
    12361 actttgacat ccagtgtacc ttgcactgac tgattcaggt catcaataat aaatgggcct
    12421 ggtggaactt ttttctggta aatcacgcga ccagaatggc ttatcgtgac cgtggcgtta
    12481 gtctgtgcga tgccgctgat ttgcggcgcg tagccgcgta actcccaggg gagcattcga
    12541 tcgtcgctgg ctaacgccgc accggtatag gagaaaccat caaaaatgtt agagctaaaa
    12601 tcggtttcgc ccagggttaa tcgggaacct atttgcggta acgggcggaa caggtaggtc
    12661 cgtgaaatcg ccccagattg ttcacggttg tcgccgctat cggactggct aagctgataa
    12721 tcgctgcgca gacgccatgc cccggcgttg aggcccgtgg tgccataggc gttcaggttg
    12781 ttgctgctgc tgccgctttg tgggcgataa gtgctggcaa acaggttgta atccatcagg
    12841 aagccaggaa taccgttatt ccacgttgag ggtggcgtcc agttttctga atgccacgcc
    12901 agccatgcct gcgggattgt aatattgagc tgttgatttg cctgatcgaa agtaaagatg
    12961 atttcaggga agacactaaa atcgacacat tcttttattg ccggtaattt tttacgaaag
    13021 tcagatttaa gtccgaagag ttcgatcagc gattcattga tacaaggaat aattttatcg
    13081 ccagattttt gccagcgaat ttgttgccca ttactgattt tattattatt aaccgtaaca
    13141 ctaacaaagt aatcgccggg agctacgacg cctttttgat tgagcaacga aatatcaacg
    13201 ctatcccgaa tcgacttatc gagaacgtta aggttaaatt cagctgtcag cgcaggaggg
    13261 gttaccattg ccagacagga aagaacagag agtcgataaa tattcacggc atccatacct
    13321 gaacaatatt cgcaaaaggc ggctaatcag ccgccttaaa aaggattatt ggtaatccag
    13381 aacgtaagtt gcgttagcag taacggcccc cgcggtaacc gaaactgcgt tatccatctg
    13441 ttccatccag gcaaagaagt ttaacgtttg ttctgttgaa cttgccaata agtcaattgt
    13501 tggcgcttct gttcccaatg taacaaatga ttcatctttg ttcatgagac gtacgccaac
    13561 gccggtagcc tcgccagcac tgatgttact aaggagcggg gttgcacctg tcgttttagc
    13621 cgtgctgtcg aaggtcacgc ctacctttga gaccacgcca cccttgccat catcccagcc
    13681 tgcaatatca cagttgatta agcgaatatt gacaggtttt ttctcgctat gatgttctcg
    13741 atttatgtgg gatgtggtta cttcacccag gtcaatgttc atatcaaggt catcagggtg
    13801 aatttcacat ggtgcttcaa taacttcacc tttaaagtgg atttcaccag atccttcatc
    13861 tgcagcaaaa acaggtgaag cgaatagtag agatatggcc agggcggcca gtgttttttg
    13921 tcctttaaac atacatgaat
//
LOCUS       DBFIFM010000122        13739 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.122, whole genome shotgun sequence.
ACCESSION   DBFIFM010000122 DBFIFM010000000
VERSION     DBFIFM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.122"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..494
                     /gene="ftnA"
                     /locus_tag="N7C74_003100"
     CDS             <1..494
                     /gene="ftnA"
                     /locus_tag="N7C74_003100"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HGU2432922.1"
                     /translation="KPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLRRH
                     AQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKINEL
                     AHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELSTLD
                     TQN"
     gene            complement(531..770)
                     /locus_tag="N7C74_003101"
     CDS             complement(531..770)
                     /locus_tag="N7C74_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HGU2432923.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            962..2173
                     /gene="tyrP"
                     /locus_tag="N7C74_003102"
     CDS             962..2173
                     /gene="tyrP"
                     /locus_tag="N7C74_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HGU2432924.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2235..2900)
                     /gene="yecA"
                     /locus_tag="N7C74_003103"
     CDS             complement(2235..2900)
                     /gene="yecA"
                     /locus_tag="N7C74_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HGU2432925.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3257..4258)
                     /locus_tag="N7C74_003104"
     CDS             complement(3257..4258)
                     /locus_tag="N7C74_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2432926.1"
                     /translation="MSVRKIPSGKWLCECYPYGASGKRIRKQFATKSEALSYERRLMN
                     SRVGDEFQDGSGPRLSELIARWFEMYGKTLSSGAERKVKLEAICSRLGDPFASQFDKN
                     MFATYRERRLSGEWNPKGKKKLSEATVNREQSYLHAVFAELKRLGEWSGENPLTGIRK
                     FREEEKELAFLYVDEIERLLIACDESRNKDLGVVVRIGLATGARWSEAEGLKQSQVLP
                     GRITFVKTKGKKNRTVPISPQLQAMLPKKRGALFSPCYEAFDAAIKRAKIELPDGQLT
                     HVLRHTFASHFMMRGGNILVLQKILGHSDIKMTMRYAHFAPGHLEAAVELNPFDNRG"
     gene            complement(4264..4611)
                     /locus_tag="N7C74_003105"
     CDS             complement(4264..4611)
                     /locus_tag="N7C74_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2511 domain-containing protein"
                     /protein_id="HGU2432927.1"
                     /translation="MKVKKVQLLVTFLSMFSFSAVAMPFKTIERESFNGVWPFNTDEV
                     QLQCLDGNPYVMNFDDNKLYALTGLARIKGKTFGALPLDNNNPFWLDNDAAPGLKKSL
                     GDVTKAAFDLCDK"
     gene            complement(4641..5291)
                     /locus_tag="N7C74_003106"
     CDS             complement(4641..5291)
                     /locus_tag="N7C74_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432928.1"
                     /translation="MAVNSNGSNNRVAGRDFHEKNIQIERYDGSHTVNIAIPSNNDDD
                     DRPLLKAQRKELNSLVAAIAEASNTEAFIIWQKVHAEIGVAGIDDMTVNQYKTAESFL
                     HAMLERCKDHDACKALVSLLLRNSEDCGLRQKLLRYCHINFGTGRLNDLTRSQLQSAL
                     SWLEQQSASSHIESSTLPEVRLRASELIRLYPKEIIFFICVGVLVGGVISRAFFNL"
     gene            complement(5307..5711)
                     /locus_tag="N7C74_003107"
     CDS             complement(5307..5711)
                     /locus_tag="N7C74_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU2432929.1"
                     /translation="MKNDIGRRLREERERLGLSQVAMSDIGGVKKLTQLRYEKGDSFP
                     DAAYLAALSRFGLDVQYVVLGIHSPETYNDDEQELITRFRAASLDVKNAVIGALKGAI
                     SEKETQPSGRELNISGGNNRIAGRDYNETKGR"
     gene            complement(5801..5938)
                     /locus_tag="N7C74_003108"
     CDS             complement(5801..5938)
                     /locus_tag="N7C74_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432930.1"
                     /translation="MRAMLEDERCSLAAISSMTARSSRDSRSASLGPVAVLRGMRDLV
                     S"
     gene            6010..6213
                     /locus_tag="N7C74_003109"
     CDS             6010..6213
                     /locus_tag="N7C74_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU2432931.1"
                     /translation="MSGITININVNAPYVSLQKYAEITGIPLNTCKKMLADGRIIIRP
                     KRAKMEKPEVNLVAMLKDALANS"
     gene            6235..6585
                     /locus_tag="N7C74_003110"
     CDS             6235..6585
                     /locus_tag="N7C74_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HGU2432932.1"
                     /translation="MKKNANNPYSKFRNGVERHVHHVATSASRSNSRYNLNETHATPD
                     GHAVKQIGEHAWLIEKAGIVVHKCPRNPFTGNRIFALNCGDNHFGQDFTLYEALRTVD
                     RLLRGQSFIKQADL"
     gene            6596..6874
                     /locus_tag="N7C74_003111"
     CDS             6596..6874
                     /locus_tag="N7C74_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432933.1"
                     /translation="MTKEHAQGVFIRFIDFRGELLLRASAIDGIVPSEKNAATYVYLN
                     GTRLTVELPYQTVREIISEAEKARQVNGDEPYIEIICMDSEAEIQKAD"
     gene            6886..7128
                     /locus_tag="N7C74_003112"
     CDS             6886..7128
                     /locus_tag="N7C74_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HGU2432934.1"
                     /translation="MGKEYKTLINKAPERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            7125..7238
                     /locus_tag="N7C74_003113"
     CDS             7125..7238
                     /locus_tag="N7C74_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432935.1"
                     /translation="MSIFISWLVLIISVACAIGIMRIIHSVKKIERFFTDK"
     gene            7331..7747
                     /locus_tag="N7C74_003114"
     CDS             7331..7747
                     /locus_tag="N7C74_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432936.1"
                     /translation="MNAKEKNIINTLKIVSAEQDKLSRAAQKDNQHMAALYALTIAIA
                     TPEAAKVIEEQSKEIDTLKTQSTVAAMNPSSIGRCIYILGSAMMLQYTIIAELHGKYL
                     ITPYHTKESELLTNLRLIERSQAVFIDDAQRAVFNA"
     gene            7771..7974
                     /locus_tag="N7C74_003115"
     CDS             7771..7974
                     /locus_tag="N7C74_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432937.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVARVNLIRQVQEPRR"
     gene            7971..8237
                     /locus_tag="N7C74_003116"
     CDS             7971..8237
                     /locus_tag="N7C74_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGU2432938.1"
                     /translation="MTILDYIATHPGCSGGEIAAALNTPTTAINAELRRLWRGGLVIR
                     TNRSTGGRARKTGGQASYHVNPMPFGCSNPLTHMFNQLLKEARA"
     gene            8234..8533
                     /locus_tag="N7C74_003117"
     CDS             8234..8533
                     /locus_tag="N7C74_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432939.1"
                     /translation="MSTINHQKLRELAFALQRMATPQKLLAFRAMLSPSAVLALLDQL
                     EHARTTAPAIRLTLHHEIADFCATLGAPGEPETPEAMQQELLQRIDNVFDFFLNQ"
     gene            8856..9086
                     /locus_tag="N7C74_003118"
     CDS             8856..9086
                     /locus_tag="N7C74_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HGU2432940.1"
                     /translation="MADRKQHRAIAERRHIQTEINRRLSRASRVAQIMHINMLHERSH
                     ALSNIYSASVFSYLADDLHEFQQLIQQQNKLH"
     gene            9159..9524
                     /locus_tag="N7C74_003119"
     CDS             9159..9524
                     /locus_tag="N7C74_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2432941.1"
                     /translation="MILANDFLEYLLNTERDLAARVRDRYDMYLKSLPVPQLADGKIV
                     IDGRYMIDSHEGNYRLYRIEGGTPSVIGIYQRPSSAIVDVIADSIRITHRHADTEDTV
                     LEIQRLATACRDTLNGMTK"
     gene            9531..12353
                     /locus_tag="N7C74_003120"
     CDS             9531..12353
                     /locus_tag="N7C74_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HGU2432942.1"
                     /translation="MTAEYIRDWQQPRHAVGREGTGIPAPESALSSWLDAYRVENERR
                     QEMADAAFSATPLGNLINKSLDAQEKQDKTITLAGDARKQARGAVDEAMASLRLLPSY
                     LRDPLIRHLSFLRKKQEADRQKGKKSWQAERYARGNLRKIFERLERTDHRWLTQGYRS
                     LAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTMDVTLKA
                     YQMLARMALHLHAMPPHYDALTTDKDRRNEPDTELLPGAILRLTCAEWWKRKLWLLRC
                     EWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFTIDLETV
                     YYAGVSNPVHRKAEMMATMKGLELLAEARGDKAVFLTVTCPSKYHATTENGHPNPKWN
                     GATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFAHPEEID
                     TIVSHTRDIAIQEDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDSRAVDGI
                     DPKTGKPRVDHETGKSMTESVERAIGWARLHRVRQFQFFGIPSRQVWRELRRLASQMA
                     RNPEGPQRLKDDAMDAVLAAADAGCFTTYIEKQGGVLVPRKDYLIRTAYDLADELNDY
                     GEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPGVEDSARENGFDLQGGPAAPWTR
                     GNNCPRVQETDNNGTEQPEERPAPWPQLPDGVDVNEWMRSLKRHERRALMRSLRDKQA
                     KNSSDEMQSWTQSRKQQRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLRGGSLYV
                     DGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLAQLGASD
                     PEAAARLASTLQQDQNTMKTPVTVLSDMLRAIRDAEHAQRISETTERASRKADLLRGG
                     LTSGNKKQTETGLTNPVNEQKTRRDI"
     gene            12430..13389
                     /locus_tag="N7C74_003121"
     CDS             12430..13389
                     /locus_tag="N7C74_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HGU2432943.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGTQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNVIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAVFSGYGRVM
                     VVGGGAEIVATAIREACGVNATFIADGVPQFALVNGLYAMNKE"
     gene            13394..13708
                     /locus_tag="N7C74_003122"
     CDS             13394..13708
                     /locus_tag="N7C74_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HGU2432944.1"
                     /translation="MTTPTRRISFYLKPAAVKSEQEACNYLDSLPASERSRAQRAAFL
                     AGLALIKRNPAFAYWMAEWPEDNLPFNTVSIKETYQANQPSGVECNFYKIKKNIQTLF
                     PE"
BASE COUNT         3676 a         3622 c         3196 g         3245 t
ORIGIN      
        1 tgaaaccaga aatgattgaa aaacttaatg agcagatgaa cctggaactg tactcttcac
       61 tgctttatca gcaaatgagc gcctggtgca gctatcatac cttcgaaggt gctgccgcgt
      121 tcctgcgccg tcacgcccag gaagagatga cgcatatgca gcgtctgttt gattacctga
      181 ctgataccgg caatttaccg cgtattaata ccgttgaatc tccgtttgct gaatattcct
      241 cacttgatga attattccag gaaacctata aacacgaaca attaatcacc cagaaaatta
      301 acgaactggc tcatgctgca atgaccaatc aggactaccc aacatttaat ttcctgcagt
      361 ggtatgtttc tgagcagcat gaagaagaga aactgttcaa atcgattatt gataaattaa
      421 gcctggcagg caaaagcggc gaaggtctgt attttatcga caaagaactc tctaccctcg
      481 acacacaaaa ctaatgctct ccggcggcag gtttctgccg ccatttcaaa tcagtgacgg
      541 caaatcttac tctgatcggt cgaaaaacct tcttctgaag gtataaattt gccttttgct
      601 gccagaaacg ctaccagctc tcccgctgtc atcccttctg ccgagcaagt gtgaaaacgt
      661 gcctgttcac caaaacgcgc tttaatcgca gcttccagac tggcatgcgt atattgctcg
      721 cctgattcaa tcatcatatt taacacttca tgaccgtgaa tagagtccat cgttcctcct
      781 caaaaaaaag cctagcgtag cgattgccgc ttatgaagac tttgcgccag cgcaggactg
      841 aatgcttttt attgtacatt tatgtttaca ccatatgtaa cgtcggtttg acgaagcagc
      901 cgttatgcct taacctgcgc cgcagatatc actcataaag atcgtcagga cagaagaaag
      961 cgtgaaaaac agaaccctgg gaagtgtttt tatcgtggcg ggaaccacaa ttggcgcagg
     1021 catgctggca atgccgctgg ctgcggccgg tgttggtttt agcgttacgt taatcttgtt
     1081 gattgggctt tgggcgttga tgtgctacac ggcgctatta ctgctggagg tgtaccagca
     1141 tgttccggca gataccggtc tgggcacgct ggcaaaacgc tatctgggac gctacggtca
     1201 atggctgacg ggcttcagta tgatgttctt aatgtatgct ctgactgcgg catacatcag
     1261 cggtgccggt gaattgttgg cctccagcat cagcgactgg acaggtattt caatgtcggc
     1321 aaccgctggc gtgttgttgt tcacttttgt tgccggtggc gtggtttgtg tcgggacatc
     1381 gctggtcgat ttatttaacc gttttctgtt cagcgccaaa attatttttc tggtggtgat
     1441 gctggtattg ctgctaccgc atattcacaa agtgaatctt ttaaccctgc cgttgcaaca
     1501 ggggctggca ctgtctgcaa tcccggtgat ttttacgtcg tttggttttc acggtagcgt
     1561 gccgagtatt gtcagctata tggatggcaa cattcgtaag ctacgctggg tgtttataac
     1621 cggtagtgcg atccccctgg tggcatatat tttctggcag gtggcgacgc ttggcagcat
     1681 tgattcaaca acctttatgg gattgctggc taatcatgcc ggattaaacg ggctgttaca
     1741 ggcgttacgc gaaatggtgg cctctccgca tgttgagctg gcagtgcatt tatttgctga
     1801 tttagccctt gccacgtcat ttctcggcgt tgcgttaggc ttatttgatt atctggcaga
     1861 tttgtttcag cgttcaaata ccgttggtgg acgattgcaa actggagcaa ttacctttct
     1921 gccaccgttg gcgtttgcac tgttttatcc acgaggattt gtgatggcgc tgggttacgc
     1981 cggtgtggcg ctggcggtac tggcattgat tatcccttcg ctgttgacct ggcaaagcag
     2041 aaagcacaat cctcaagcgg gttaccgggt caaaggtggt cgtccggcgc tggtggtggt
     2101 gtttctctgt ggtattgctg tgattggcgt gcaatttttg attgcggcag ggttgttacc
     2161 agaagtgggg tgattagata gcctcaaatt ccttattggg tgccagaatt aacgctgaca
     2221 cccaatttga cttcttaatg caggcagcac tgcttaaatt ttttaccact accgcacggg
     2281 caaggatcgt tacgccccgg tttctcttct gctttgatcg gttgctgaac agctttttcc
     2341 tgcggatgcg ccatccagta cgcatgtaga tcaagcgccg ccagtcgaat ggcatctacg
     2401 ctctcttcaa acgcttctgg cgacatcttc tctacccgct cgaagttttc ctcagtaccg
     2461 tgcagcgcta tcgcctccag cgctggtttt aacgaatcgg gcaatgttga ccagtcagaa
     2521 agtgccacgc cccgcatata gccaaagcac cactcctcaa caatcgtcag ctcgctgcca
     2581 tcaacttctc gcaagccgaa taacggctca aactgctccg ggaattcgtt cagacgctct
     2641 gcggtatcgg ccatatgttg aaaagccagg ttcataaagc gcgtcatctc tttctctgac
     2701 gcccagcgcg gcacatagtc agccccaccc cacacggcaa ccagccactg tgccggttca
     2761 atctcttgcg gagaactcaa caccgccgtc aataaaccgt ccagctccgc cacatcaagg
     2821 atggcgtggt cagtgttgta tttggtcaga atatcgtcca gccattccaa ctcactttcg
     2881 tttaacggtc ccgttttcat acgcttttcc ttgtggatct caactcgcca gcacctatct
     2941 tacatgccgg tccgtatcag agatactttt tgagtggctt tgccggtgat taaaaatcaa
     3001 ggagggtgta acgacaagtt gcaggcacaa aaaaaccacc cgaaggtggt ttcacgacac
     3061 tgcttattgc tttgatttta ttcttatctt tcccatggta cccggagcgg gacttgaacc
     3121 cgcacagcgc gaacgccgag ggattttaaa aactatcaac caccatttat aaatcataac
     3181 cttatgattt tattaacttt gaaaatggct ctatacaacg ccatttgaat ctattgtcac
     3241 tttttatcgc cactttttac cctctattgt caaaagggtt caattcaaca gcagcctcta
     3301 aatgacctgg agcaaaatgc gcataacgca tagtcatttt tatatcgcta tgccccagta
     3361 ttttttgcaa cacaagaata tttccacccc gcatcataaa atgactggca aacgtgtgac
     3421 gtagcacatg agttaattgc ccatcaggaa gctcgatctt cgctctctta attgcagcgt
     3481 caaaagcctc ataacatggt gaaaatagcg ctcctcgttt tttaggaagc atagcttgca
     3541 attgaggtga aatcggtaca gtgcggttct tctttccttt agttttaaca aatgtgattc
     3601 gaccgggcag tacttgagat tgctttaatc cttctgcttc actccaccga gcaccagtcg
     3661 caagcccaat acggacaaca acccccaaat ctttattccg tgactcatca cacgcaatca
     3721 gaaggcgttc aatctcatct acatacagaa acgccagttc cttttcttcc tcacgaaact
     3781 tgcgaatacc agtcaggggg ttttcaccag accactcccc aaggcgcttc agttcggcaa
     3841 aaacagcatg tagatatgac tgctcgcgat taacggttgc ttcactaagt tttttcttcc
     3901 ccttgggatt ccattctcct gatagccttc tttcccgata agtagcaaac atatttttgt
     3961 caaactgaga agcaaatgga tctcccagcc tggaacaaat cgcctcaagt ttgactttgc
     4021 gctctgcacc agaggacaag gttttaccgt acatctcaaa ccaacgagca atcaactcag
     4081 aaagacgagg accagaacca tcttgaaact cgtctccaac tctactattc attaaacggc
     4141 gctcataaga gagcgcctca ctttttgtcg caaactgttt acgaatgcgt tttcccgatg
     4201 ccccgtaggg ataacattca caaagccatt tacctgatgg aatcttacga accgacattt
     4261 tagttactta tcacataaat caaatgcagc cttagtgaca tcccccagac tcttttttaa
     4321 ccctggggcg gcatcattat ctagccaaaa tggattattg ttatctaacg gtaacgcacc
     4381 aaatgtttta ccttttattc gagccaaacc tgtaagtgca tataacttat tatcgtcaaa
     4441 attcatcaca taaggattac catcaagaca ctgtaattga acctcatcag tattaaatgg
     4501 ccatacccca ttgaaactct cacgttcaat agttttaaaa ggcattgcga cggcggaaaa
     4561 agaaaacata gataaaaaag taactaatag ttgaaccttt tttactttca tatcattacc
     4621 tcaatttagc tcaagtaaga ttacaaatta aaaaacgccc tagaaatgac accgcctacc
     4681 aaaaccccta cgcagataaa gaatattatt tcttttggat aaagtcggat taattctgaa
     4741 gcacgaagtc ggacttctgg taaggtcgaa ctctctatgt ggcttgatgc cgattgttgc
     4801 tctaaccacg acaatgcaga ctgtaactga gaacgagtaa gatcgtttaa acgtcctgta
     4861 ccgaaattga tatggcaata ccgcagaagt ttttgtcgaa gtccacagtc ttcactgtta
     4921 cgtagtaata aacttacaag agccttacag gcatcatgat ctttacatcg ctcaagcatt
     4981 gcatgcagaa aactctccgc tgttttatat tgatttactg tcatatcatc aataccagct
     5041 acaccaatct ccgcatgtac tttttgccaa ataataaacg cttcagtatt gctagcttct
     5101 gcaatagcag caaccaagct atttagctcc ttacgctgag ccttaagcaa agggcgatcg
     5161 tcatcatcat tattcgaagg gattgcgata ttgacggtat gagaaccatc atatcgctct
     5221 atctgaatat tcttttcgtg aaaatcacgc ccagcaacgc gattgtttga accgtttgag
     5281 ttgacggcca tgtcacctcc ctactatcac ctacccttag tttcgttata gtcacgacca
     5341 gcgatacggt tattaccacc agaaatattt aactcacgtc ctgatggctg agtttccttt
     5401 tcactgatcg caccttttaa agccccaatc accgcgtttt tcacatctaa cgaagctgct
     5461 cgaaagcgag taatcaattc ctgctcatca tcgttataag tttcaggtga gtgaattccc
     5521 aacacaacat actgaacatc aaggccaaaa cgagacagcg ctgccaaata cgcagcatca
     5581 ggaaagctat ctcctttctc atatctaagc tgagttagct ttttgactcc accaatgtcg
     5641 ctcatggcaa cttgactaag tcccaatctt tccctttcct cacgcaaccg ccgaccaata
     5701 tcatttttca tacaaaaacc ttgacaggta tcttttttga taccaaaatg atttcacgag
     5761 ctattagatg atcacaatat accactatga aacaagttct tcacgatacc agatcacgca
     5821 ttccgcgtaa caccgccaca ggtccaagac tggcacttcg gctgtccctc gaggagcgag
     5881 ccgtcattga tgaaatggca gctaaagaac aacgctcatc ctctaacatg gcgcgcatga
     5941 tctcccttcg cggcctagag ctaacccaga aagaacaaaa caaatcttcc tgatcaggag
     6001 gctagtggga tgtcaggtat aaccatcaat atcaatgtga atgcccccta tgtatccctg
     6061 cagaaatatg cagagataac aggtatccct cttaatacat gcaaaaagat gttggctgac
     6121 ggtcgaatta ttatccgccc caaacgcgcc aaaatggaaa agcctgaagt aaaccttgtg
     6181 gcgatgttaa aagacgcttt ggctaacagc taaaacaatg aacagagcac catcatgaaa
     6241 aaaaacgcta ataatccata ctccaaattt cgtaatggcg tagaacgcca tgtacaccac
     6301 gtcgctacca gtgcatcacg tagtaacagt cgctataacc tgaacgagac gcatgcaaca
     6361 ccggatggcc acgctgtaaa acaaatcggc gaacatgcct ggctgattga gaaagctgga
     6421 atcgtggtcc acaaatgccc acgcaatccg tttaccggga accgcatttt tgcattgaac
     6481 tgcggcgaca atcacttcgg gcaggatttc acattatacg aagcacttcg cacggttgat
     6541 cgtctgcttc gcgggcaaag ttttattaaa caggctgatt tataacaggt gctttatgac
     6601 caaagagcat gcacaaggtg tatttatccg ttttattgat tttcgcggtg aactgttatt
     6661 acgcgcatca gccattgacg gaattgttcc atccgaaaaa aacgcagcta cttacgttta
     6721 tctgaacggt acgcgcctga ctgtggagct tccgtaccag accgtacgag aaatcattag
     6781 cgaagctgaa aaggctcgtc aggttaatgg cgatgaaccc tatatcgaaa ttatttgtat
     6841 ggattcagaa gctgaaattc agaaagcaga ttaaagggcg ttgcgatggg caaagaatat
     6901 aaaactctca ttaacaaagc acctgaacga ttttattttc gcttaagtgc atcaggcgct
     6961 catgctgaac gtgcagcccg tgactcattg accagggcaa tccggagttt gtatgacgtt
     7021 gctttttacg ctgatgatct ggatgcactt aacgaacttt ccgagctgat ctgtgccgca
     7081 gaatgcgggg aacacattga accatataag ctggggaata tcgcatgagt atatttatct
     7141 catggcttgt tctgattatt tcggtggcct gcgccattgg gattatgcga attattcatt
     7201 cagtaaaaaa gattgaacgc tttttcactg acaaataaca gcttaaacag aacaccagat
     7261 taaacccgaa aacctgaaaa ctatccgcat tcgcggaggt attcgcacac gtaaataacg
     7321 gagatataaa atgaacgcaa aagaaaaaaa tattatcaat acattaaaaa tagtgtcagc
     7381 agagcaagac aagctgtcca gggcagccca gaaagataat caacatatgg cagcgcttta
     7441 cgcactgacc atcgcaatag ccacccctga agcagccaaa gttattgagg aacagagcaa
     7501 agaaatcgac actcttaaaa cacagtcaac agttgcagcc atgaatccgt ccagcattgg
     7561 acgctgcatt tatattcttg gttcagcaat gatgctgcaa tacaccatta ttgccgaact
     7621 gcacggcaaa tacctgataa cgccttacca cacaaaagag tcagagcttc tgacaaatct
     7681 ccgcctgata gaacgctctc aagctgtatt cattgatgac gcgcaacgtg ccgtatttaa
     7741 cgcatagggt tactggacaa agggggcgca atggcaatta agcattttcc cgtcgttcgc
     7801 tttacctcca gagggcgcga atacgaggtc gacgaacgcc tgattaccac tatcgacaaa
     7861 catcgttcgg aaaaggatgc acaccacatc tacctcactg acggcactta cttctgcgcc
     7921 accaacgtgg cgcgagtaaa tcttatccga caggtacagg agccacgcag atgaccattc
     7981 tggactacat cgctactcat ccggggtgta gcggcggaga gatcgccgca gcactgaata
     8041 ccccaaccac agccattaat gctgagttac gccgactttg gcgcggcggc ttagtcatca
     8101 gaacaaaccg cagcacaggt ggtcgcgctc gcaaaacagg aggccaggct tcttaccacg
     8161 taaacccgat gccgttcggg tgtagcaatc cacttactca catgtttaac cagctactga
     8221 aggaagccag agcatgagca ccatcaacca ccagaagcta cgcgaactgg catttgccct
     8281 gcaacgaatg gcaacgcctc aaaaattact ggcatttcgc gcaatgctct cgccgtctgc
     8341 tgtgctggca ctgctggatc agctggagca cgccagaacc acggctcctg ccattcgcct
     8401 cacgctccat catgaaatcg ctgatttctg cgcaacgctg ggggcacctg gcgaaccgga
     8461 aacgccggaa gcaatgcagc aagagctgct gcaacgcatc gataacgttt ttgatttttt
     8521 tctgaaccag taagaaacca gaacatgcac acacaaaaaa accgcttgcc atgccgcaat
     8581 cggtcaggtt acatttccgc tgcacctcat aaaacgggtg ccgggattct caacccgata
     8641 cagagcaaag cgcataaccg cgctagcggt ttttttgtgc gtactgtatt gccacgtctt
     8701 tttcgcgtca gaattatggc ggggcgtacg gggccgactt cggtcgggcc ggattctttg
     8761 ctctccggtg ttgagaaccc tgtacgtctc gccaccccga gattctcaac tctggatggt
     8821 gagttatttc tatcaccgag caaagaggcc acaccatggc agaccgcaaa cagcaccgcg
     8881 ctatcgcgga gcgtcgtcac atccagactg aaatcaaccg cagactttcc cgcgcatcac
     8941 gcgtcgcgca aatcatgcac atcaatatgc tgcatgagcg cagccacgca ctatcaaaca
     9001 tttattccgc ctctgttttc agctatctgg cggatgatct gcacgagttt caacagctca
     9061 tccagcagca aaacaaactc cattaattcc tgttccgggc ctttcctgca ccttgcggcg
     9121 ggaggccttc gcacatctgt aacaagagga ttgccgcaat gattctcgcc aacgactttc
     9181 ttgaatacct gctcaacaca gaacgtgatc ttgccgctcg cgtgcgtgat cgttatgaca
     9241 tgtacctgaa atccctgcct gtaccgcagc tcgctgacgg aaagattgtt attgatggtc
     9301 gctacatgat tgacagccac gagggaaatt acaggcttta ccgcattgaa ggtggcaccc
     9361 cgtccgttat tggcatttac cagcgcccat cctctgcaat cgtcgatgtg attgccgaca
     9421 gcatccgcat cacacatcgc catgccgaca cagaagacac cgtgctggaa attcagcggc
     9481 tggctacagc ctgccgcgac accctgaatg gcatgacgaa gtaaatcact atgacggcag
     9541 agtacatcag ggactggcaa caaccgcgcc acgcagtggg gcgtgaagga acggggatcc
     9601 ccgctcctga atccgcgctt tcctcctggc tggatgccta ccgggtagag aacgagcgcc
     9661 gccaggaaat ggctgatgcg gcgttctccg caacgccgct gggcaacctg attaataaaa
     9721 gcctggacgc acaggaaaaa caggacaaaa ccatcacact ggcaggagac gccagaaaac
     9781 aggcacgcgg tgcggtggat gaagccatgg cctcgctgcg cctgctgccg tcctatctgc
     9841 gcgatccgct tattcgccac ctctccttcc tgcgcaaaaa acaggaagcc gatcgtcaga
     9901 aaggcaaaaa gagctggcag gctgaacgct acgcgcgcgg aaacctgcgc aaaatattcg
     9961 aacgtctgga gcgcaccgat caccgctggc tgacacaggg ttatcgctcc cttgccggac
    10021 gcgaacgcct ggacgatttg ctttacctgc cgcagctcaa caaacaccag atacagacgc
    10081 tggccaccat gacggcggcg atgttcagca gcaccttcga aaaactctgc gatggctttg
    10141 gcgcgaccga tggcgaactg accatggatg taacgctgaa ggcgtatcag atgctggccc
    10201 gcatggcgtt acacctgcac gccatgcctc cacattatga cgcactgaca acagacaaag
    10261 accggaggaa cgaaccggac acggagctgc tgccgggcgc aatccttcgc ctgacctgtg
    10321 cggaatggtg gaaacgcaaa ctgtggctgt tacgttgcga gtggagagaa gaacaactcc
    10381 gcgccgcctg tctggtttcc agaaaaacat cgccctatct gagccaggac gcgttaagcg
    10441 agtttcgcgc acagcgcgag aaaacacgcg atttcctgaa aagtttcatg ctggaaaacg
    10501 aagacgggtt cacgattgat ctcgagacag tgtattacgc gggagtaagt aacccggttc
    10561 accgtaaggc agaaatgatg gccaccatga aggggctgga acttctggcc gaagcccgtg
    10621 gcgacaaagc ggtgtttctg actgtcacct gcccgtcaaa ataccacgct acaacagaga
    10681 acggtcatcc gaatcccaaa tggaacgggg ccaccatgcg cgactccagc gattacctgg
    10741 ttaacacgtt ttttgcggcg gtccgcaaga aactgaaccg cgacggcctg cgctggtatg
    10801 gcatccgcac ggtggagcct caccatgacg gcaccgtgca ctggcatatg atggtctttg
    10861 ctcatccgga agaaatcgac accattgtgt cccacacccg cgatattgcc attcaggaag
    10921 atcgtcacga gctgggcgat gatattactc cgcgctttaa ggcggagtat gtcgacggct
    10981 caaaaggcac gccaaccagc tacatcgcca cctacatcgg aaaaaacctg gacagccgcg
    11041 ccgtggatgg catcgacccg aaaacaggca aaccacgcgt tgaccacgaa accggaaaat
    11101 caatgaccga gagcgtggaa cgcgccattg gctgggcgcg ccttcaccgg gtccgccagt
    11161 tccagttctt tggcatcccc tcccgtcagg tgtggcgtga actgcgtcgc cttgccagcc
    11221 agatggcacg caacccggaa ggcccgcaac ggctgaagga tgacgcaatg gatgcggttc
    11281 ttgctgccgc tgatgccgga tgttttacca cctacattga gaaacaggga ggcgtacttg
    11341 ttccacgcaa ggactacctg attcgcaccg cctacgacct cgcagatgag ctgaacgatt
    11401 acggcgaaca gagcgtacag atttacggga tctggtcacc actcatcggg gaatcctccc
    11461 gtgtgtgcac gcacccggat aactggaagc tggtaagacg taaaccggga gtagaagaca
    11521 gcgcccgcga aaatggtttt gaccttcagg gcggccctgc cgccccttgg actcgtggca
    11581 ataactgtcc ccgtgtacag gaaacggaca acaacgggac agaacagccg gaagaacggc
    11641 cagcaccgtg gccgcagctt cctgacggcg ttgacgtgaa cgaatggatg cgctcactga
    11701 aacggcacga acgccgggcg ctgatgcgtt cgcttcgtga caaacaggca aaaaacagca
    11761 gtgatgaaat gcagagctgg acacagagcc gcaaacagca gcggcctttg cctgataacc
    11821 acgaattact cgctaaagaa tggcgggagt ctgctgaatc tctcggcctg catatcggtg
    11881 aacaacagat gcagcacctg ttacggggcg gcagtctgta cgttgacggc agcatcattg
    11941 caccgcaggg atttgaaatt gtacgcaaac cggatacccg cccggacagc cgaatcacgc
    12001 agctctggca gcgcctgagc cgtaatcatg gcgtaagcag cacggagatc cgccataacc
    12061 cggtcgccag ctatctggca cagctggggg catcagaccc tgaagccgcc gcacgcctgg
    12121 catccacact tcagcaggac cagaacacca tgaaaacacc cgttaccgtg ctttctgaca
    12181 tgctgcgcgc catccgcgac gcagagcacg cacagagaat cagtgaaacc actgaacgcg
    12241 ccagccgcaa agcagacctg ctgcggggtg gcctgaccag tggaaacaaa aaacagacag
    12301 aaacgggact cacaaatccc gtaaatgagc aaaaaacgcg ccgcgatata tgaagcgcgc
    12361 acaaaacagg caaaagcggg atttaaaaat cctgtaaccg attaattaat caacataagg
    12421 aaaagcgaca tgaaaatttg tatcgacgac ggctccacca acatcaagct ggcatggact
    12481 gagaacggcg aacgccgcaa cgccatcagc ccgaacagct tcaagtcgga atggtctgcg
    12541 ccattcggtg gcacgcagcc cgcgaactac atgcttgatg gcgtgcgcta tggttttgat
    12601 ccggtcagcg atcgctttgt ccagacgacc gacacgcaat accagtacag cgatgtgaat
    12661 gtaattgcca ttcatcacgc gctggtcaaa tcaggcatca caccacagga agtggatgtg
    12721 gttgtcaccc tgccactgag cgagtatttc gatacaaacg cacagccgga catggccaac
    12781 atcaaccgca aaaaagcgaa cgtcatgcgc ccggtggagt accagaacgg cgaagcattc
    12841 actatccgta acgtacgggt tatgcctgaa tccattcctg ctggctttaa ggcactggct
    12901 gacatgagtc cgtttgaatc cctgctgatt gtggatttgg gcggaaccac gctggatgtg
    12961 gcaaaggttc aggggcaact ggcaggtatc agccaggtgt tttgcgatcc acacgtaggc
    13021 gtttctctga tggccgatgc cgtactgtcg gtgatggcca ctaacggtat gcgcaccagt
    13081 caccacatcg ccaataccat tatcgaacat cgccatgatg aagcctggct gcgccagcac
    13141 atccacaatg acgcgcatta cgccagcctg atggcggtta ttcgtgaaaa ggaagaaaca
    13201 ctgaaacaac gcgtgatccg cgcgctggcg gttttttcgg gttacgggcg ggtgatggtt
    13261 gtcggtggcg gggcggagat tgtggcaacc gctatccgcg aagcctgcgg agttaatgcg
    13321 actttcatcg cggacggggt gccacagttt gctctggtta atgggttgta cgcaatgaac
    13381 aaggagtaaa ccaatgacga caccaaccag acggataagt ttctatctga agcccgccgc
    13441 cgtcaagagt gaacaggagg cgtgcaatta cctcgatagc ctgccagcct ccgaacgcag
    13501 ccgcgcgcaa cgcgcggcct ttctggctgg gctagcactc ataaagcgca acccagcatt
    13561 tgcctactgg atggccgaat ggccagagga caacctcccc ttcaataccg ttagcatcaa
    13621 ggaaacatac caggctaatc agcctagcgg agttgaatgc aatttttata aaataaaaaa
    13681 gaatattcag accttattcc cagaataatc tgataattgt atgtgaacta ataaaatac
//
LOCUS       DBFIFM010000123        13576 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.123, whole genome shotgun sequence.
ACCESSION   DBFIFM010000123 DBFIFM010000000
VERSION     DBFIFM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.123"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            214..1044
                     /locus_tag="N7C74_003123"
     CDS             214..1044
                     /locus_tag="N7C74_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HGU2432945.1"
                     /translation="MSMPLSNVLQSQIITDNHFLHHPKVESELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1185..1958)
                     /gene="uxuR"
                     /locus_tag="N7C74_003124"
     CDS             complement(1185..1958)
                     /gene="uxuR"
                     /locus_tag="N7C74_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HGU2432946.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2173..3633)
                     /locus_tag="N7C74_003125"
     CDS             complement(2173..3633)
                     /locus_tag="N7C74_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HGU2432947.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3714..4898)
                     /gene="uxuA"
                     /locus_tag="N7C74_003126"
     CDS             complement(3714..4898)
                     /gene="uxuA"
                     /locus_tag="N7C74_003126"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HGU2432948.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SH"
     gene            5238..6581
                     /gene="gntP"
                     /locus_tag="N7C74_003127"
     CDS             5238..6581
                     /gene="gntP"
                     /locus_tag="N7C74_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HGU2432949.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6755..7657)
                     /gene="fimH"
                     /locus_tag="N7C74_003128"
     CDS             complement(6755..7657)
                     /gene="fimH"
                     /locus_tag="N7C74_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HGU2432950.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7677..8180)
                     /gene="fimG"
                     /locus_tag="N7C74_003129"
     CDS             complement(7677..8180)
                     /gene="fimG"
                     /locus_tag="N7C74_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HGU2432951.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8193..8723)
                     /gene="fimF"
                     /locus_tag="N7C74_003130"
     CDS             complement(8193..8723)
                     /gene="fimF"
                     /locus_tag="N7C74_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HGU2432952.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8733..11369)
                     /gene="fimD"
                     /locus_tag="N7C74_003131"
     CDS             complement(8733..11369)
                     /gene="fimD"
                     /locus_tag="N7C74_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HGU2432953.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAVQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLARNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11436..12161)
                     /gene="fimC"
                     /locus_tag="N7C74_003132"
     CDS             complement(11436..12161)
                     /gene="fimC"
                     /locus_tag="N7C74_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313302.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HGU2432954.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     IIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGARVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12198..12737)
                     /locus_tag="N7C74_003133"
     CDS             complement(12198..12737)
                     /locus_tag="N7C74_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2432955.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTPPGGNMQFQGVIIAETCL
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12802..13350)
                     /gene="fimA"
                     /locus_tag="N7C74_003134"
     CDS             complement(12802..13350)
                     /gene="fimA"
                     /locus_tag="N7C74_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGU2432956.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQDGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSEQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         3304 a         3483 c         3261 g         3528 t
ORIGIN      
        1 tagtaataag atatgatttt aaattgcatg caaataattt taaatgagcg ttttttgata
       61 ctttagagtg tttcctaccc tttaatatca ctgtgtagag ttgatatcac tacaacagaa
      121 acatgcatgt catttgttta cttctctttt tactacagaa aaatttatct atttttctaa
      181 acatcagtga aatactcata aaaggtaact attatgtcta tgccattaag caatgtcctt
      241 cagagtcaaa ttattacaga taatcacttc cttcatcatc caaaagtcga aagtgagtta
      301 acacgtaaat atgaacgtgc acgcctggat acggaaaata tctatcttct gcctcttgct
      361 cgtggcaata accataatta cgatggaaaa agcgttgtcg aaattagaaa acttgatatt
      421 agtaaagaat cctggccatt taattatgtg acagaagcat gcagagaatc cgatggtatt
      481 acaacaacag gacgtatgct ctatcgaaac ttgaaaatca cctcagcttt agacgaaata
      541 tatggtggta tatgtaaaaa ggcgcatgct gctacagagt tagcagaagg tttacggcta
      601 aatttattta tgaagagccc tttcgatcct gttgaggact atacagttca tgaaataaca
      661 ttaggccctg gttgcaatgt tccaggttac gccggaacca cgataggata tatttcaacc
      721 cttccagctt ctcaggcaaa aagatggacc aatgagcagc caagaattga tatctatatt
      781 gatcagataa taactgtctc aggggtagca aattcttccg gattcgccct tgccgcatta
      841 cttaatgcta atattgagtt gggtaatgac ccaataattg gtatagaagc ctatccaggt
      901 accgcggaga tccatgcaaa aatgggatat aaagttatcc ccggtgatga agatgcgcca
      961 ctcaaacgca tgactctgca accgtcatct cttccagaac tgtttgaatt aaaaaatggt
     1021 gaatggaatt atattggcaa ataaccactg agaagaatga gtactaacat tcaggttata
     1081 tagatattta aacaataccc tgcaatttac aaagtaatac tcattgcgac ttcttaattt
     1141 aagaaagcgc aaggaacgtt tacccttgcg cttattataa taagtcaggc gtcgacttta
     1201 tccagcggcc atgaatcaaa cagatagccg tcaaaataaa tatcgtcaac gttcgagaat
     1261 tccagcagac gttgcttaac gttttccaga tgctgccaca ttgccagctt cgccgctcgg
     1321 gcatcttttt tgattaacgc ggcgaggatc tgtttgtgat cgcccaacca ctctttgcga
     1381 tacaggctgt catccagatg gctgtgcaac tggatccaca ttgggttgtt ttcccgccac
     1441 tgccaggact gacggaacag ctccaccagc atgctgttat gcgttgcttc ggcaatagcg
     1501 agatggaact gcatgtcacc gctttcgctg ctgcccggcg cactggaagc cagttcacgc
     1561 tcttccagtt gcaatgcctg acgcattttg acgatatctt cgcgggtagc ctgcagggcg
     1621 gcaaactcgg cgatgttact ttccagtaac tgacgcgcct gtaacagctc aaaaggaccc
     1681 gcatcattgc agacgttggc atccggactg tctgtgttgt ggctgcctga gctatcaaga
     1741 acatagatac cggcaccccg gcgcacttcc accagccctt tgatctccag catgatcagc
     1801 gcttcacgta ccaccgtccg cgtgacatca agcatttctg caatttcacg ctccggcggt
     1861 agccgttcac caggattgta cggcgtcttt atgatcagat cgcggatcat cgccccgact
     1921 tcctggtaag gtctttgcgc agaggtggca gatttcatgg tgttgtgtcc gttaggtgcg
     1981 gttaatcaaa tttttgccgg atgcggcgtg aacgccttat ccggcctaca acgggcaatt
     2041 ttacggcccc cgttaatctc aaactatacc attagcactg gaagttggca cgtacaggtg
     2101 ggcaaaaaaa cgaaaactgg tcaaccaaat atcagtttga ttgaccagta agtctgtagg
     2161 gtaatcagtt agttacagcg cagccacaca ctcgcgcgca ccgcgttcgc acagctgctg
     2221 atatgccgca gtcaccgcgc caacaaagtc ggcattctgc ggcagatcat cggcaaaaat
     2281 accgctcagg cccagcagcg ctttcacgcg gtctgcgccc tgatactgcg cgttaatctt
     2341 ctggaactcg gccagcatcg ggtcgaccac gtcaatggca ttaccctgct catccacgcc
     2401 ctgggtgtaa cgcatccagc cagccacgcc cagcgccagg tgacgccagc tgccgccgtt
     2461 ttgcaggtgc agacgcaccg ggtccagcag acgctgcggt aacttctggc tgccgtccat
     2521 cgcaatctgc caggtacggt gacgcagaga cgggttgctg aaacgctcga tcagcagcgt
     2581 cgcataggcg ttcaggtccg taccttccgg catcgacagc gttggcgctt gttcctgcat
     2641 catcagggca aaggccgctt tgcgataatc cgggttagtc atggtgtcgg caatagtttc
     2701 atagccgccg aggtaaccga ggtacgccag gaaagagtgg ctgccgttca gcatacgcag
     2761 tttcatcatt tcgaacggca caacgtctgc aacgaactgt gcgcccactt tatcccagtc
     2821 cggacgacca ttaacgaagt tatcttcaat aacccactga cggaacggtt cgcaggcaat
     2881 ggcgcacggg tcgtaaacac ccagctggtc agcaatttcc tgtaaggttt ctggcgtcgc
     2941 cgccggaacg atgcggtcaa ccatggtgca cgggaaggtg acgttctctt caatccatgc
     3001 cgctagctgc gggtcacgcg cctgagccag ccccagtacc gcgaccttcg ccacatgacc
     3061 gttttcacgc acgttatcgc aggacatcac cgtaaatgct ttcagccctt tttcacgacg
     3121 cagacgcaag gcttcgacga tgtaaccaat cgcggacttc ggcgcagtcg ggttttccag
     3181 atcgtgcttg atcagcgggt tattgagatc cagttgaccg cttgccgcat cagcgcagta
     3241 gcctttttcc gtgaccgtca gggagacaat cgccgtttgc ggacgcgcca tcgcgttgag
     3301 aataccttcg cagccgtcga tttccgggtg cagcgcttct ttcatcgaac cgataatttt
     3361 cagctcggtg ctttccgcgc ctttttccgc cacggtgtac agcagttgct gttttttcag
     3421 gttttcgatc agcacgcggt cgttgcctgg catcaggtta acttcgcaga taccccagtc
     3481 gctgtcggtg ctttccagca gatgatgggt gtacagcgcc tggtgcgcac ggtgaaacgc
     3541 cccgcaaccg agatgcacaa tgcgtgattc cagacgagaa tgatcccatg acgggcgggc
     3601 aaccggcaga ttgctgtcaa caatagtagt catcgtaaac tccagaatcc gtcttcagag
     3661 agtgacggca aggaagaagg agccggcacg gcgcgcagcc atgccggtgg gtattaatgg
     3721 ctaaagaaag cgcgctggat cgccagttcg acaccgcgaa cttcggccag gcctttcaga
     3781 cgaccaattg cggagtaacc agggttggtt ttcttcttca ggtcgtccag catctgatga
     3841 ccgtggtccg gacgcatcgg gatcaggtct tctttgcctt ccgctttacg acggtgttct
     3901 tcttcaacaa tcgctttcac cacttcgtac atatcaacgt caccgttcag gtgcgccgct
     3961 tcgtggaagg ttttcgggtt atcttcacgc atggtggagc gcagatgggt gaagtaaata
     4021 cgcggaccga actgcttgat catatcaacc aaatcgttgt cagcacgcac gccgtaggaa
     4081 ccggtgcaca tggtgaaacc gttcgccatg ctgtttacgg tatcaaccat ccactgcata
     4141 tcttcaatgg tggaaacaat gcgcggcagg ccgaggatcg ggcgcggcgg atcgtccggg
     4201 tgaacagcca tacggacgcc aacttcttcg gcaaccggaa taatcgcttt caggaagaca
     4261 gcaaagtttt cgcgcagttt ggctttgtcg atatctttgt acagctccag gtgcttacgg
     4321 aactggtcga gggtgtaccc ttcttccgca cctggcagac cagcgatgat gttacgggtc
     4381 agacgcgctt tgtcttcatc gctcatagtg gcgaagcgtt cagctgcctg agcaatttct
     4441 tcttcggtgt aatccgcttc cgcgcctgga cgtttcagga tatgcatttc gaatgcagcg
     4501 aattcgatct ggtcgaagcg cagagctttg gagccgtctg gcagcacgta ttcgaggtca
     4561 gtacgggtcc agtcgagcac cggcatgaag ttgtagcaca cggtgcgaat gccgcactgc
     4621 gccaggttgc gcagagtctg ctgatagtta gcgatccact gctcatagtt gccagtgtgg
     4681 gttttgatat cttcgtgaat tggtacgctt tctacgacag accacaccag gcctgcgtct
     4741 tcaacgatcg ccttgcgttt gaggatctct tctacggacc atacttcgcc gttcgggata
     4801 tggtgcagcg cggtaaccac gccagttgcg cccgcctgac ggacatcagc taaagaaacc
     4861 ggatcgtttg ggccgtacca gcgccaggtc tgttccatga tttttcctct ttcacatagt
     4921 gggttccaca gcaaagagac gcggaaccct ggagttgttt aggtatccgg atgaacaacc
     4981 agaccgcagc cgtggcgttg catcggatga gatggcgtat aagttctacc ttttggtaag
     5041 acatgtcaac ccgaattctg aaattggtta accacatcac aagaatttca ctctcatggt
     5101 tgttgctgca aattgcagca aagggtaatc gatggttatt gatcagagga tgtgacattc
     5161 atcgcaacaa tggttgacca atttacataa catgtcgccc aaaataacaa cggttcaacc
     5221 aggtaaggta aaacgttatg catgtgctta acattctctg ggtggtattc ggcattggtc
     5281 tgatgctggt actgaatttg aagttcaaaa tcaattcaat ggtggctttg ttggtggcgg
     5341 cgctgtccgt cgggatgctg gcgggcatgg atttgatgtc gctgctgcac accatgaaag
     5401 cgggcttcgg caacacgctg ggggaactgg ctatcatcgt ggtgttcggt gcggtcatcg
     5461 gtaaattgat ggtcgactcc ggcgcggctc accagatagc gcatacgctg ctggcgcgtc
     5521 tcggtctgcg ctatgtacag ctgtcggtga ttatcatcgg cctgattttt ggtctggcga
     5581 tgttctatga agtggccttt atcatgttag cgccgctggt tattgtcatt gccgccgaag
     5641 ctaaaattcc gttcctgaaa ctggcgatcc cggcagtagc agctgccact accgcacatt
     5701 cactgttccc accgcagccg ggtccggtgg cgctggtgaa tgcttatggc gcagatatgg
     5761 ggatggttta tatctatggc gtactggtga cgatcccaag tgtaatctgc gcaggtctga
     5821 tcctgccgaa gttcctcggc aatcttgagc gcccaacgcc atcattcctg aaagcagatc
     5881 aaccggtaga tatgaacaat ctgccctctt tcggcgtttc gattctggtg ccgctgatcc
     5941 cggcgatcat tatgatctct accaccatcg ccaatatctg gctggtaaaa gatacccctg
     6001 cctgggaagt ggttaacttt atcggttcct cgccgattgc gatgtttatt gcgatggtgg
     6061 ttgcattcgt actctttggc accgcgcgtg gtcatgacat gcagtgggtg atgaacgctt
     6121 ttgaaagcgc ggtgaagagt attgcaatgg tgattctgat catcggtgcg ggtggcgtgc
     6181 tgaagcaaac tatcatcgac accggcattg gcgacaccat cggcatgttg atgtcccacg
     6241 gcaatatctc gccctacatt atggcatggc tgatcactgt gctaattcgt ctggcgacgg
     6301 gtcagggtgt cgtttcggcg atgaccgccg ccgggattat cagtgctgca atccttgatc
     6361 cagcaactgg tcagctggtt ggcgtgaatc cggcgctgct ggtgctggcg acggctgcgg
     6421 gttccaacac cctcacccac attaacgatg catctttctg gttgttcaaa ggttactttg
     6481 acctgtcggt aaaagacacg ttgaaaacct ggggtctgct ggagctggtc aactccgtgg
     6541 ttgggctgat tattgtgctg attattagca tggtagcgta aaagaacacc agagcctgcc
     6601 aatggcaggc tcagttcgga ttgtcggcaa agtgcgcttt gtttatgcca gatgcgacgc
     6661 tgacgcgtct tatctggcct acaaagggct aacgtgcagg tttttagctt caggtaatat
     6721 tgcgtacctg cattagcaat gccctgtgat ttctttattg ataaacaaaa gtcacgccaa
     6781 taatcgattg cacattccct gcagtcacct gcccgccggt acgtgcgtaa tttgccgtta
     6841 atcccagact taccgccgaa gttcctactg ctcctaacga taccgtgtta ttcgctggaa
     6901 taatcgtacc gttgcgcgtc aactgtacgc cgacgccctg cgctggtgaa aacgacgcgg
     6961 tattggtgaa aatcgagttg cccgcatctg cggttgtgcc ggagaggtaa taccccaggt
     7021 tttggctttt cgcacaataa acggtaagag gaattggcac tgaaccaggg tagtccggca
     7081 gagtaacggt gacatcacga gcagaaacat cgcagccgcc agtaggcact accacatcat
     7141 tattggcgta aatattccac acaaactgga aatcatcgct gttatagttg ttggtctgtc
     7201 gcaaaataag cacggcaatt aatgagccag ctttaatcgc caccccgccc gcactgctca
     7261 caggcgtcaa ataaagcgcc accggccacg gcttatccgt tctcgaatta taaacaaccc
     7321 gcggcgtttc gctggtggtc ggaaatggat agctactgcc actatatttt acggtcccgg
     7381 aaaaattaga taacacgccg ccataagccg agcctcgttg cagtgtgaca tagtctgtaa
     7441 tggtttccgg ataatcgtta tggcaaaaga tttgcgtcga aagatctacg accaggtttt
     7501 gccccacatt cacggcaggc gcaaggttta cataaacatt agcgctgcca ccgccaatag
     7561 ggatagcggt accattggcg gttttacagg cgaatgacca ggcatttacc gaccagccca
     7621 tcagcagtac agcaaacagg gtaataactc gtttcattac aattatctct tcgggttcag
     7681 ctgtaggtat aggtgatgct aatcactgcc tgaatggttc cctgagtggc tccgccattt
     7741 accgtcaatg ctctgacctg taacgggaag tgcgctgatt gtgaggaatc atccacctga
     7801 actgttttgg ttgcgccagt attcaatgtg ttgccactgt catcctgtag ctctaactgg
     7861 atgttttgcg cggtcccctg gtttttataa tatccggtac tgtcggctgc cccgctgaag
     7921 ctggcagtga cccttgacgt tcccaccgga caattagtca actcaagcgc aacatcatgc
     7981 caggccgatg ccgccccggc agacatcaga ctgaaagaat aaagatcgcc gagatcaacc
     8041 gtggcattgg tggtggaaac tgtgcacggt ttggcgacga ccttaccgtt caccgtgatg
     8101 gtgacatcgg ctgcctgtat cgtcgcactt gcgagcgcca atattgccgc caataaatac
     8161 ccacgtttgc gccatttcat gagcatctcc agttactgat attcaagagt gaaggtagcc
     8221 gtggcattga tatgccccgc agtgacaggc acctgtgtcg ccattagccg ggcgtaaaaa
     8281 ttcaacgtat ttggtttacc cggcgtcagg gtcgtccacg aaatcgcgga cgatggagca
     8341 ttaaggggta tctgattttg ctgctcattc agaagctgta ttcccagtcc cgaagccgct
     8401 gacaccgtat tttcaagtgc aagcaggttg gcattgtggc tatctgcaac gccggtaaac
     8461 ccaactttta cggcagaaac ggcgttacca cagggtgaca gcaaaatacg aaatggaacg
     8521 acaggagtcg tcgcgccaat gttgttaaat tgcttcgccg cgttttccat cagatcaaca
     8581 gtaaaattgg ttgattcagc ggccacacta cagccgttat ctctgacata gccgcggata
     8641 gtaatcgtgc tatccgcagc caaagcgtga ctcactgcca gccacaaaaa agcgcacaga
     8701 agataaaaag gtttgtttct catcacgccc ccttaacgac attcagctga tagctgggtt
     8761 aataactgct gctgactctc tggtggcagt tgataattgg cgacacaatg agcattttcc
     8821 tcttctcccc atttcacctg aacttttccc gctagaggca ttccgctgag gtaaacctga
     8881 ccattatccg caacaatgcc gctactctgg ctactctctg atgtcaccat cgccccaaac
     8941 ggcagcggct tattattgtg ggttagcgtc atgagcagtt ttatcccaac gcgcgcttta
     9001 aactctgctc gcacgatcgc cccacgagtg ggaacaacgt tagcgaccgc gttatctaaa
     9061 tcgacgttat cagccagggt attggtatcc agcgccactc tattttcccg atattcagtg
     9121 gcataaggca gcacggcata accgcgccag tcggtacgca cccccgtctg gttttcgact
     9181 tttgcatctt ttgcgccagg cgctttaaca agcaccaccg tatcgtttaa cggctgcccc
     9241 agcgttacgc cattggcatg agccagtacc ccaccgctga ctccgtaata gagctgctta
     9301 atatcatcgc tatggctgta accgatattg gcattgccgt aaccaccgcg ataattcagc
     9361 gtggcgtagc ctgtgcttcc gctattacca tcgcctcccc cggcatagcc ggtttgcacg
     9421 ctatagctga ggttgttgtc ttccagcaac gtaccgtata caccagccag attggtcatc
     9481 cgaccgttga gatcgtgtga catgctgtag ctggcactgg catgtcgcca ctgagattta
     9541 ctgtcagaac gcagccagtg gctgaaagga atattgacgt tacgcgctaa catctgatca
     9601 cgtccttttt gccaggcgtt tttcgtcagg ctatagctga gcgtccagtt gatatcttcg
     9661 aacgcagtat ttaatccagc ctggaattgc tcatcgacat tactcgttcc ccaataagtt
     9721 tgatggctac cactcaaata cagtgttgat gagcgcccga gttgctgagt aacggtgagt
     9781 tgtaatttcc cgcgtttgtt ataagcgagg ttgtaatagt cggtgaattt cggcttaacc
     9841 tgaataactc cgtcctgtgt ttcgatgttg tagccattca ttcgactgta tgttgtatca
     9901 gcgaaattaa aatatccgct ggtcgaataa cggtaaccca ctaactgaat attcgtgcct
     9961 gactcattga gcgatttgtt atagagaaaa cgcaccgatt gtccgtcatg ctgactgtca
    10021 tcgggaagtg tggaattagc ctgagtcata tccacagaca gagcgcccag tgcccccata
    10081 tttttcccga taccaaaatt aaaagcacga taacgatctg ccagttgcgt tccaccatat
    10141 attgtccagc cagctggaag gccgtggagt aatgtacttt ggaaaaagcg gggtttttcc
    10201 tgttgcgcat ttccactacg gtattctcct gccgtaatgg aataacgagt atgcccttca
    10261 cgttgcaaaa gcgggactga cgaatagggt acggtaaaaa tctgcgtgct gccgtcagcc
    10321 tctttaatcg ttacctgcaa gtcaccacta ttacctgcgg catagatatc gttgatggta
    10381 aaaggccccg gcggcaccgt actattataa atgtcatacc cattttgttt aatagtgacc
    10441 tgtgcagtac cacgagcaat accgtggatc accggggcaa atcctctttg gctatcgggt
    10501 aacatattgt catctgaggc caattgtgcg ccgcgaaagt taataccatc gaaaatatca
    10561 ccctgagtat aaccatcacc cagcgtcagc cgggaacgta acggtattat gtctcgctca
    10621 agccaggtat tgatatgctg ccatttattt ttgctacctg atgatctgtc gctactgtta
    10681 taactccagg tggtattgtc gcgtaaacgc cacgcaccaa tatttaaccc actctgtagg
    10741 tttaaatatg cataatggct gttaccccca atccgattct gtacactatt tccgctgaaa
    10801 ttataattga gcaatccggc attaataccg ggatcccata actcaggagg aatataacca
    10861 cgcgcgcgat tactcataaa tgcctgaggg atcgtcaggt tcagtcgctg ctgaccaaca
    10921 tctaaatgcg cagtagcgtc ctggaccatt gtggttaatg gcacacaggc atcatccgcc
    10981 agcagattca taccggcgac agaagccgta ttcagcccca tactggcgag ttgcgcgcgt
    11041 gtcaggcagg gaacaatccc ttgttcactg tcgcccgtat taaatgtgac atcacgcgtt
    11101 gccatataac cattattcaa atagatatcg acgcgatacg tccctggcgg taattcttgc
    11161 ccattttcaa aacgcgataa atcggccaca gcctggggat catccgctaa aaagcgcgga
    11221 ttaaaataga gttcggcaga tgacaaaggt gcctgtacgg caaaagcaca ggcgacaaag
    11281 agccggacaa aaaaaccagc caaacgatgc ttacgaatat gcaagcattg tgtgtttcgc
    11341 tggtaaagtc ttaaattcag atatgacatt attcctccgg tttaaaatca cccggcagtc
    11401 aattcttttt attcaggcga aaaattcccc ctgcgttatt ccattacgcc cgtcattttg
    11461 ggggtaagtg caccataatc atttattgtt cggtaagtaa tattgcttcc cgcatcagaa
    11521 ggcaatttaa ccgtgctttc gcccattgga ggcaccaagg cattttcaag aacccgggct
    11581 ccggcattca actctgttac cgtcaggtaa tagggtgtcg ggttaatcag cgtcagagaa
    11641 ttcgcgctac gacgaaatct taatttttct gcggcctgat cgggtggcaa cgctaattta
    11701 gccggacgat agtacagttt aatgcggctg ataattgcga gctgtagcgt attctcagtc
    11761 aattttgatt tatccattga cggaatcgct ttaacgttca tccagaataa actttcccgg
    11821 tcctgtggca attggttatt tgttgcatca agaatacgta aggtattctc tttttttccc
    11881 ttcatcgcaa acagaggagg cgtcacgata aaacgaccat cctttacacc atcggcattt
    11941 tccacccatg attgaattaa ataggtacta ttttcatcat tatttgtcac ggcaagttgc
    12001 acttgttttt gccctgccgg ataaattatg cgagtcgcac ctaaggccac tcccgcttca
    12061 gcgcgtccgg caaccatcat tgccatgaac atcaggatac ctgccagcaa gcagaatgtt
    12121 atttcctgcg atttccttac attgacgttt ttattactca ctgtcctgtt cctgttatca
    12181 cattatctgc tgaacaatta ctgataggtt aaagagaacc aggcctgtgc attggcgatg
    12241 ccgccagtaa cccgacgccc ggtagcacga tatttggcga tgaaatgtag cgaagtagag
    12301 cctgagtaaa gccgtttcca gtttgctgga ggacgattaa tcggtacgag gtttccttca
    12361 tcatcaaaca acgctacgcc aatattggtg gctatccctg gcccctctcc cacggaaagc
    12421 acatccggat ttttaccatc cgcgacaccg tgaaacgcca cacccacacg ttcactcacc
    12481 accgtgctac attcccgtaa atgaataaca aaaggcaccg gtgcgctatc ttcccctacc
    12541 gcatgaaacc ggttactgct gatttgcccc atattgaccg tcatttgttt atcaccggct
    12601 tcaatcaggc aagtttccgc aataatgacg ccctgaaatt gcatatttcc gccgggcggc
    12661 gtggtattcc atttatttcc ggccagagca aacattggca gcaacgacgc taatagaaat
    12721 aatctcttcc ttttcatcgt tatttttatc gcacaagggt gggcatccct gcccgtaatg
    12781 acgtccctga acctgggtag gttattgata ctgaaccttg aaggtcgcat ccgcattagc
    12841 agcacccggg gttgcctcgc cgattgcata ataacgcgcc tggaacggaa tggtgttagt
    12901 accgttattc agggttgttt gctcactgaa tgtcgcacca tccagcgtca gcgcagcacc
    12961 cgttctgtcc aggatctgca caccaacgtt tgttgcgcta cccgcagctg aactctgcag
    13021 agccagaacg ttggtatgac ccgcatcaat cgccgtacct aaaaaggcaa cagcggcttt
    13081 agatgcaaca ttggtatcgc aatcattcag ctgaatgtta aaaccgacag cagaactggt
    13141 tgctccgtcc tgtgccagcg atgcggtacg aacctgtcct aactgaacgg tttgatcaac
    13201 agagcctgca tcaactgcgc aagcggcgtt aacaacttcc cctttaaagt gaacggtccc
    13261 accattaacc gtcgtggcag cggccagagc cgctgtagaa ctgagggaca gagccgacag
    13321 aacaacgatt gccagagttt taattttcat gctgctttcc tttcaaaaaa ctatttctaa
    13381 atcgacatgg gcagtcgttc tgtacacttt gttttgtcaa cgagtttgac tgccaacact
    13441 gcacagtttt cccccaaaag atgaaacatt tggggccatt ttgactcata gaggaaagca
    13501 tcgcgcacaa actttttcag tttatttgct ggattaatat tctattgtta tctttattta
    13561 tagattttta tattgc
//
LOCUS       DBFIFM010000124        13425 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.124, whole genome shotgun sequence.
ACCESSION   DBFIFM010000124 DBFIFM010000000
VERSION     DBFIFM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.124"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(8..84)
                     /locus_tag="N7C74_003135"
     tRNA            complement(8..84)
                     /locus_tag="N7C74_003135"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48..50),aa:Met,seq:cat)
     gene            293..1390
                     /gene="mltA"
                     /locus_tag="N7C74_003136"
     CDS             293..1390
                     /gene="mltA"
                     /locus_tag="N7C74_003136"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HGU2432957.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1629..2435
                     /gene="tcdA"
                     /locus_tag="N7C74_003137"
     CDS             1629..2435
                     /gene="tcdA"
                     /locus_tag="N7C74_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HGU2432958.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINSECCV
                     TVVDDFVTPDNVAQHMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2486..2929)
                     /gene="csdE"
                     /locus_tag="N7C74_003138"
     CDS             complement(2486..2929)
                     /gene="csdE"
                     /locus_tag="N7C74_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HGU2432959.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2929..4134)
                     /gene="csdA"
                     /locus_tag="N7C74_003139"
     CDS             complement(2929..4134)
                     /gene="csdA"
                     /locus_tag="N7C74_003139"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HGU2432960.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGIVVIVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4326..4553
                     /gene="ygdI"
                     /locus_tag="N7C74_003140"
     CDS             4326..4553
                     /gene="ygdI"
                     /locus_tag="N7C74_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HGU2432961.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4904..5821
                     /gene="gcvA"
                     /locus_tag="N7C74_003141"
     CDS             4904..5821
                     /gene="gcvA"
                     /locus_tag="N7C74_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HGU2432962.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5840..6235
                     /gene="ygdD"
                     /locus_tag="N7C74_003142"
     CDS             5840..6235
                     /gene="ygdD"
                     /locus_tag="N7C74_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HGU2432963.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6228..7328
                     /gene="rlmM"
                     /locus_tag="N7C74_003143"
     CDS             6228..7328
                     /gene="rlmM"
                     /locus_tag="N7C74_003143"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HGU2432964.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7372..8103)
                     /gene="fucR"
                     /locus_tag="N7C74_003144"
     CDS             complement(7372..8103)
                     /gene="fucR"
                     /locus_tag="N7C74_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HGU2432965.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTG"
     gene            complement(8161..8583)
                     /gene="fucU"
                     /locus_tag="N7C74_003145"
     CDS             complement(8161..8583)
                     /gene="fucU"
                     /locus_tag="N7C74_003145"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HGU2432966.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8585..10003)
                     /gene="fucK"
                     /locus_tag="N7C74_003146"
     CDS             complement(8585..10003)
                     /gene="fucK"
                     /locus_tag="N7C74_003146"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HGU2432967.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPLKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(10112..11887)
                     /gene="fucI"
                     /locus_tag="N7C74_003147"
     CDS             complement(10112..11887)
                     /gene="fucI"
                     /locus_tag="N7C74_003147"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HGU2432968.1"
                     /translation="MNKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11920..13236)
                     /gene="fucP"
                     /locus_tag="N7C74_003148"
     CDS             complement(11920..13236)
                     /gene="fucP"
                     /locus_tag="N7C74_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HGU2432969.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGSHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
BASE COUNT         3231 a         3326 c         3546 g         3322 t
ORIGIN      
        1 ttttaattgg ttgcgggggc cggatttgaa ccgacgacct tcgggttatg agcccgacga
       61 gctaccaggc tgctccaccc cgcgtccgtg gatgcgcact atactccgct cgcgttttca
      121 tgcaaccttt tttttacccc gatcacggat ctggcatgat tttgaacaaa aacaaatcac
      181 taaagcctat tttttgtgca aaatttgcgt cagggagagg ctgctcattt cattaaacgc
      241 ggtggtttgt tatcttcgtt gcgccttatt ttttaacctg aagaagagaa caatgaaagg
      301 acgttgggta aagtaccttc ttatgggcac ggttgtggca atgcttgccg cctgctcttc
      361 caaaccaacc gatcgcggac agcaatataa agacgggaaa tttacccagc ctttctctct
      421 ggtgaaccag ccagatgccg ttggcgcgcc gattaacgcc ggtgattttg ccgagcaaat
      481 taaccatatc cgtaattcgt caccgcgtct gtatggcaac cagagtaatg tttataacgc
      541 ggtgcaagag tggctgcgcg caggcggtga tacccgcaat atgcgccagt tcggcattga
      601 tgcctggcag atggaaggtg ccgacaacta tggtaacgtg cagtttacag gctattacac
      661 gccggtaatt caggcgcgcc atacccgcca gggcgagttc cagtatccta tttaccgtat
      721 gccgccaaaa cgtggtcgtc tgccgtctcg tgcggagatc tacgcgggtg cattgagcga
      781 taagtacatc ctcgcttaca gcaactctct gatggataat ttcattatgg atgtgcaggg
      841 tagtgggtat atcgactttg gtgatggcag tccgcttaac tttttcagct atgcagggaa
      901 aaacggtcat gcctatcgca gcattggtaa ggtgctgatc gaccgtggcg aagtgaaaaa
      961 agaagatatg tcgatgcagg cgattcgtca ttggggcgaa acacacagtg aagccgaggt
     1021 tcgcgagctg ctggaacaga acccgtcttt cgttttcttt aaaccgcaat cttttgcacc
     1081 ggtgaaaggg gcaagtgcgg tgccgctggt tggccgtgct tctgttgcct ctgatcgttc
     1141 cattatccca ccaggtacaa ccttgctggc agaagtgccg ctgctggata ataacggcaa
     1201 atttaacggt cagtacgaat tgcgtctgat ggtggcgttg gatgtcggtg gcgcaatcaa
     1261 aggccaacac ttcgatattt atcaagggat cgggccggaa gccggacacc gcgcaggttg
     1321 gtacaaccac tatggacgtg tctgggtgct gaaaaccgcc ccgggcgcag gtaacgtctt
     1381 tagcggctga tgtgatattc tgtatgccgt ttttacggat atgacagggt gaggggtaac
     1441 ctcacatcct gattattgac taagtgcgcg ttgttcatgc cggatgcggc gtgaacgcct
     1501 tatccggcct acaaatcatg caaatccaaa atattgcagg agttatgtag gcctgataag
     1561 cgtagcgcat caggcattta tgcgtttagg gtgagggata acctcgccct ttttaattct
     1621 gaggtgctat gtctgtggta attagtgatg catggcgtca gcgttttggt ggcacagcgc
     1681 gtctgtatgg tgaaaaagcg ttgcaactgt ttgctgacgc gcatatttgt gtggtcggta
     1741 ttggcggtgt cggttcctgg gcggcggaag cgctggcgcg cacggggatt ggcgcaatca
     1801 cgcttatcga tatggatgat gtgtgcgtca ccaataccaa tcggcaaatt catgccctgc
     1861 gcgataacgt tgggctggca aaagcggaag tcatggcgga acgtattcgc cagattaact
     1921 cggagtgctg tgtaacggtg gtggatgatt tcgtgacgcc ggataacgta gcgcagcata
     1981 tgagcgtggg ttattcgtac gtgattgatg ccattgatag tgtacggccc aaagcggcgc
     2041 tgattgctta ttgtcggcgc aataaaatcc cgctggtcac gaccggtggc gcgggtgggc
     2101 agattgaccc aacgcagatt caggttaccg atctggcgaa aacgattcag gacccgctgg
     2161 cggcgaagtt gcgtgagcgc ctgaaaagcg attttggcgt agtgaaaaac agtaaaggta
     2221 agctcggtgt ggattgcgtg ttctctaccg aagcattggt gtatccgcag tcagacggta
     2281 cggtgtgtgc gatgaaagcc acggcggaag gaccaaagcg gatggattgt gcatctggat
     2341 ttggcgcggc aacgatggtg accgccacct ttggttttgt tgcggtttct catgcgctga
     2401 agaagatgat ggcgaaagcg gcgcgtcagg ggtaaacaat caggtcggat gcgacgcaaa
     2461 cgtcttatcc gacccggttc tgtgcttaaa cctgcttcgt cgcagcgata atcgcctcgc
     2521 ttaacgcatt taacccctgg ctgcgtgagg cgctaagttg cgcacgcaat cccagctcat
     2581 caaacaatgc cagcggcgac tgtgcctgca attcggcggc ggttttcccc tcaacggcag
     2641 tcaacagcac cgccaggagg ccgcgcacta tacgtccttc gctgtcgcca aagaagtgca
     2701 ttttaccgtt ttcagccacg gtatatccca gccagacgcg gttttcgcat ccggcaatct
     2761 ctttagcctg cgcttttaac tcgtctggca aagccggaag ctgtttcccc agcatgatca
     2821 actggcgata tttatcttcc cattgcgtca acggtgcgaa ggtattgcgt aacgtttctg
     2881 cggttacggt tgtgccgaac ggatgtccgg cgaattgcgg gttcgtcatt aatccaccaa
     2941 taattccagc gcgcggtcaa cggcattcac cagcgcatcc acatcactct ttgtattata
     3001 tggcgcaaaa gaggcgcgca gtgtgccggt cacgcctaat tctgccagta gcggctgagc
     3061 gcaatgttgc ccggcccgca aggcaatacc gtactccgcc agcagcgtca ccatatcgct
     3121 gtgatgaacg ccagcaaaat caaaggccag caggctggaa tcctggcagc ggaatgaacg
     3181 aaagcctgga cgtttcgcca gcgcatcttc cgccagcgtt gctaagctac ggctccagtt
     3241 ttcggcctgg ttgatatcgt aatctgccag ccattccagc gccgcgctta atcctatgac
     3301 accagccaca tttggcgttc ctgcttccag tttccacggc gcagattgag tcgtgaagcc
     3361 gtcaaaactc acttcgtgaa ccattttgcc gccgcccagc cagggcgaca tcgcttccag
     3421 cagttctgat ttgccataca gcacgccgat acccgtcggg ccatacagtt tgtgaccaga
     3481 gaaagcatag aaatcgatat ccagttgctg aacatccgcg gggaaatgca ccgccccctg
     3541 agcaccatca actatcacca ctatcccggc tgaatgagca aaggtaatcg ctcgcgccag
     3601 atccgggcaa ccgccagtta cgttcgacat ctgacccaac gccagaatcc gactacgggg
     3661 agtaatcagt tccggcagca aatcgacatc tggcagtcgc tgcgcattaa gcggcaattt
     3721 gaccactttg gctccagttt gttgggcgac catcagccag gggacgaggt tggcgtggtg
     3781 ttctgccacg ctgacaataa tctcatcgcc cggttgcaga cgcggacgcg catagcattg
     3841 tgccaccatg ttgatggatt cagtggtgcc gcgcgtccag acgatagttt tatcatccgg
     3901 tgcattcagt aattgcgcca ctttctctcg tgcagcttca taacgcgcgg tcaggcgttg
     3961 ggcttcggca aactggctgc gatggacgtt tccggcgctc agactgtaaa actgtcgggt
     4021 ggcttcaacc acggcttcag gtttaagcgc ggtcgcggcg ctgtcgagat agacgcccgc
     4081 atcctgtaga gcgggaaact gggcgcgaaa ctgcgcggga ttaaaaacgt tcatggtact
     4141 cctcggcttg atgcaccgat cgtgacgcaa aattcacttt gatacaagga gagtgctaac
     4201 catcagaatt atctggatat cctggcgaag ccgcgtcagt aacatatgct gaatagtgtt
     4261 aggcgttaat tttagcctgt taatcaatac tattaagatt tattaatcgc tttaaggaga
     4321 agatgatgaa aaagactgcc gcaattattt ctgcctgtat gctgactttt gccctgagcg
     4381 cctgttccgg ttcgaactat gtgatgcaca ccaatgacgg acgtaccatc gtctctgacg
     4441 gcaaaccaca gactgataac gataccggta tgatttcgta taaagacgct aatggcaaca
     4501 aacagcagat caaccgtacc gacgtgaaag agatggtcga actggatcag taattcgcga
     4561 tcgcaaggta aaaaaaagca ccgcaattag gcggtgctac attaatcact atggacagac
     4621 agggtaaatg tacaggaagt gaaaaaaggt agctttgcta ccatggtctg aatcgcagac
     4681 caattgcaaa cacaacaaca caacatcaca accgtaagcc aaaagttcac cagaacacgc
     4741 attccgataa aacttttcgt tccggctcag gaagtgccgc cactataggt atttgctggt
     4801 agaagctcaa cggacaattt ataatggctc agattaaaaa aactaatagg ttacacagtg
     4861 tgatctaatt gttaaattca tttaacatca aagtttaaaa gccatgtcta aacgattacc
     4921 accgctaaat gccttacgag tttttgatgc cgcagcacgc catttaagtt tcactcgcgc
     4981 agcagaagag ctttttgtga cccaggccgc agtaagtcat caaatcaagt ctcttgagga
     5041 ttttttgggg ctaaaactgt tccgccgccg taatcgttca ctcctgctga ccgaggaagg
     5101 gcaaagctat ttcctcgata tcaaagagat attttcgcaa ttaaccgaag cgacgcgtaa
     5161 actccaggcc cgtagcgcca agggggcgtt gacggtcagt ttactcccca gtttcgccat
     5221 tcattggttg gttccgcgac tttccagctt taattcagct tatccgggaa ttgacgttcg
     5281 aatccaggcg gttgatcgtc aggaagataa gctggcggat gatgttgatg tggcgatatt
     5341 ttatggtcgg ggcaactggc cggggctacg ggtggaaaaa ctgtacgccg aatatttatt
     5401 gccggtgtgt tcgccgctac tgctgactgg cgaaaaaccc ttgaagaccc cggaagatct
     5461 ggctaaacat acgttattac atgatgcgtc acgccgtgac tggcagacat atactcgtca
     5521 attggggtta aatcatatca acgttcagca agggccaatt tttagccata gcgccatggt
     5581 gctgcaagcg gctattcacg ggcagggagt ggcgctggca aataacgtga tggcgcaatc
     5641 tgaaattgaa gccggacgtc ttgtttgccc gtttaatgat gttctggtca gtaaaaacgc
     5701 tttttatctg gtttgtcatg acagccaggc agaactgggt aaaatagccg cctttcgcca
     5761 atggatcctg gcgaaagccg ctgctgaaca agaaaaattc cgctttcgtt atgaacaata
     5821 atttacgtag ggtacgacca tgaccagccg ttttatgctg attttcgccg ccattagcgg
     5881 cttcattttt gtggcactgg gcgcttttgg cgcgcatgtg ttaagtaaaa ccatgggggc
     5941 cgttgagatg ggctggatcc agaccggcct cgaataccag gcgtttcata cgctggcgat
     6001 cttaggtctg gcggtggcaa tgcagcgtcg catcagtatc tggttttact ggagtagcgt
     6061 tttcctcgcg ttaggcacgg tgctgttcag cggcagcctt tattgcctgg cactgtccca
     6121 tctgcgtttg tgggcgtttg tcactccggt tggcggcgtg agcttccttg cgggctgggc
     6181 gttaatgtta gttggtgcta tccgtttaaa gcgcaagggc gtaagtcatg aataaggttg
     6241 tattgctgtg ccgtccgggc tttgaaaaag agtgcgccgc agaaattacc gataaagccg
     6301 gccagcggga gattttcggt tttgcccgcg tgaaagagaa tgcgggttat gtcatttatg
     6361 aatgttatca acctgatgat ggcgataagt taatccgtga gctgccgttc agttcattaa
     6421 tttttgcccg ccagtggttt gtggtggggg aactcttgca gcatttgccg ccagaagatc
     6481 gtattacccc cattgtcggc atgttgcagg gcgtagtaga gaagggcggt gaactgcgtg
     6541 ttgaagttgc cgataccaac gaaagcaaag agttgctgaa attctgccgt aaattcaccg
     6601 tgccgctacg cgctgccttg cgcgatgccg gggtgctggc gaactatgaa acgccgaagc
     6661 gtccggttgt gcatgtattc tttattgcac caggctgctg ctataccggt tactcataca
     6721 gcaacaataa ttcgccgttc tatatgggca ttccgcgcct gaaatttccg gcagatgcgc
     6781 cgagtcgttc cacgctcaaa ctggaagagg catttcatgt gtttattcct gcggatgagt
     6841 gggatgaacg cctggcgaac gggatgtggg cggtggattt aggcgcttgc cctggcggct
     6901 ggacctacca actggtgaag cgcaacatgt gggtttattc cgtcgacaac ggcccgatgg
     6961 cgcaaagtct gatggatacc ggacaggtga cgtggctgcg ggaagacggt ttcaaattcc
     7021 gtccgacgcg cagcaatatc tcctggatgg tatgcgatat ggttgaaaaa ccggcgaaag
     7081 ttgcggcatt gatggcgcag tggctggtta atggctggtg tcgtgaaacc attttcaacc
     7141 tcaaactgcc gatgaaaaaa cgctacgaag aagtgtcaca caatctggcg tatattcagg
     7201 cacagcttga tgaacatggc ataaatgctc agattcaggc gcggcagttg tatcacgatc
     7261 gcgaagaagt gacggtgcac gtccgccgta tctgggctgc ggtgggtggt cgtcgcgacg
     7321 agcgataaca ggcaaggccg gggcgtcgtc tccggcctgc taccctttcc gtcagcctgt
     7381 taccaaagaa gttgcaacct ggcgttcatc agaaataatg tgcgttacct catccagatg
     7441 cccgatgcgg gcttcccctg aacgattaaa tttactttta tcaatcaata acaacgattg
     7501 cgcggcgcgt tttaatagca ttgatttgta atcagcgttg atagcattgg agtcccacag
     7561 tgcgccgctg ctatcgatcc cttcacaaga aaaaataaac agatcgattt ccagcgattt
     7621 aagttgggaa atcagcgagg gattgacgta acagccatat ttgcgctcaa gtgtgccgcc
     7681 ggaactgatc agttgaatgc gttcgcgttt accgagttca tggcaaatcg gatggctatt
     7741 ggtgaagacc tgaatgttga tgtcaggcaa ctggcgtgcc agataccagc aagttgaact
     7801 ggcgtctaag gctatcacca tcccttcttc aatccacgcg agcgcctcgc gcgcgatatc
     7861 tgctttatgc gcataatggc ttttcagcct gatgtgaaag ggatcgccac tgtcttgatt
     7921 ttgacggtgg atatatttag cgcgtccatg attgcgcaga attttaccct gcgtctgtaa
     7981 ttcattgaga tcgcgacgaa tggtttcttt actgaccttt agctgttcag agagagcttc
     8041 cgtggtcagg ctggtatggt tcagcagcag gtcgactatc gcttgctggc gtgccgcttt
     8101 catatttttc accccatcat tcgataaccg cccgcgtcat gcgggcgtac cggtatgaga
     8161 ttacggtgtt accccttttt ttaaaagaat attcccgtac ttcgctcgtt cgcctgtgat
     8221 aacgatcgca aaggcttttt gcgcccgttc ataaaacgca aaacgattga tgcggatgat
     8281 gtcaggacac ggggcttgta gtgaaagcgc attacggtaa cgtcgttcta cttcaggatc
     8341 gagagtgtca ccttctaccg ctgccatcat caccagcggc ggtgcataac tgtccagctc
     8401 aaataacggg ataatcgcct ggagcaagtc gctcaccaac aggccatcag cgcggatcac
     8461 ctgcggtccc atcgaatggg cgggaaagtg agcatcggaa aaaataattt catctccatg
     8521 tcccatctct gccagcactt tcaatagttc gggagaaatt aacggcgaaa ttgttttcag
     8581 catttcacac ttcctctata aattcaggtt cagtttgcgg gtagaaataa cggtactgat
     8641 aatgaatctg tgcacgggct tcttccgggc tgttaaattc ccctacgcca taccaaccga
     8701 acagcgcagc tcctgcgacg gtcgtttcgg cgtcgtcgag aacttttaac ggaatatcaa
     8761 gcatattggc tttaatctga ttccacaatg tgttgcgact tcctccaccg actaacaata
     8821 attcagaggc cttaaagtgc ccgatttttt ccagcatctg tagattgcgc tgtaattgcg
     8881 cagttaaccc ttccagcgcc gcgcgataga aatgcccccg cgtggtatta agcgtcactc
     8941 cttgccagcc agcgttctga cacgacaata aatcacactg cattttcacg ccatccgcgc
     9001 caggcgcgat cagacgagcc tcttcaatca acatttgcca gggtgtttca gccgtccaga
     9061 acagttttct cacccattcc agcacaccgg atgccagcca ttgcatacct gggttataca
     9121 accctgcctg gctatccagt tcgcaggtgg aaccggcgta ctgacttaac agcgaagtat
     9181 caacctgggc gctgcgaacc attaaaattt cccatgtacc ggaagagagc acgggttcat
     9241 tttgttcagc accagcgcca aaaagggcga actgggtatc gtgacctgcg gaaatcaccg
     9301 gtatgccaac gggtaagccg agcattgctg cggcgctgtt ctgtagcgta ccaatctgtt
     9361 cacccgcttc caccagacga gggaagagtc ggcgtggaat accggtggct tgtaaaattt
     9421 gcggactgaa atcgcgttgc tggatatcca gcatctggct ggttccggcc atcgtgatat
     9481 cagtagtgaa ttcgccggtt aaacggtggt taatcagcga cgaaataaag agccaggcgt
     9541 gcgcgcgttc cagcagttgt ggatgatttt ctttcaacca caccaactta tataacgtat
     9601 tgaaactaaa ggctccgacg ccagaaatag cctgcaaccg ctgtgcggag attaaccgtt
     9661 caatattgtc cataaccgct gctgttcgcg gacatttcca gctaatgatc ggatagagca
     9721 gattgccttg cttatctacc agagcgccat ccacaccaaa ggtggtgacg gcgataccgc
     9781 ggatgtggca ttcagtcagt tcactattga tttgccgaca gcaatcagca aagcgttgca
     9841 aaatggcgtc taaagaccac tggtgccagg tgttgttttc catcgcgata tcgctggcat
     9901 taggcgttga ggcgcgggca acaatttttc cctgccgatt aaccgcgatg gccctgacat
     9961 tggtcgcgcc acagtcgagt accaggatca cttcttgttt catgatcgct cctgcaatat
    10021 agccggataa cattgcttat ccggctaacc actcttgctt gttcaggccc ggcagccgga
    10081 gcgaccgggc atcacatcag ggagtaatgt attaacgctt gtacaacgga ccgtagttct
    10141 ggcaagcgcg gtaatcctgg ccttcaatat ccatgccgtg cgcagcccag gcagaaggac
    10201 gatacacttt ggtctcttca acgttgtgca tacataccgg gatacgcagc atggaggcga
    10261 gagtgataaa gtctgcgcca acgtggccga tggtcagaac cccatggtta gcgccccagt
    10321 tcgccattac cgagtacaca tccgtaaacg gccctttacc ggtgaggcgc ggtgcaaacc
    10381 aggtggttgg ccaggttgag ttggtgcgtt tgttgaggat gtcatgcaca tccttcggca
    10441 attccacgct ccagccttcc gcgatttgca gtaccggtcc caggcctttg atgatgttga
    10501 cacgagtcat ggtgaacggg acgccgcctt cggtaaggaa gcgggaagag taaccgccgc
    10561 cacggaagta ttcgtggatc gccgggcacc attcggtagc ggcgaggcaa gcgtcagcct
    10621 cttgctgaga gatttcccag tgcggcttca tcgtcgggtt accttcgctg tcgcgttgtt
    10681 tacaggaacc gtccagcgca gcagaaccgg agttgatcaa atggatgatg ccgtgttctg
    10741 ccagtccatc cagtttatgc cccgttacac gctcaattgc ttctggtgac cagtaggtac
    10801 gcacatcggc aaatacctga gcggtgccgg tgagctggtg acccattagc attgccacgc
    10861 cgttaagact gtcgttttcg gtcgccacga caaagggttc gcgcacgcca ttccagtcaa
    10921 atgaactgtt gaggatcgct tcggcggtgt caccattggg atattgatcg gtccagtgac
    10981 gttgcccctg gaagcccgca gcgatggcgt tgtagccaag tgattcttcc acgcgaccaa
    11041 tatcggccag tttgctgttg ccttgcatca tgtcgcggat acacatcgcc atcagtaaac
    11101 tttcgcgcag aactgcgcgg ctttgctcgg cattgcgttg atactgtttg ttattttcat
    11161 cttcgccata gcggaagttt ttatcagccc aggccagtgc catttccagt tcggcttcgt
    11221 cgtaaatctt ctgatcgata cggcgacgca gttcggtcat atccaccgcc tggactttca
    11281 ttcccagcca ggattcaaag aagttgtgat caacaataga accggcgata cccatcgaaa
    11341 cgccgcccag cgacagatag cttttacctt tcatgctggc gacggccaaa ccggcgcggg
    11401 caaagcgcag cagtttttct tcaacatcgg caggaatcga tgtgtcatcg gcatcctgaa
    11461 cgtcatgacc gtaaatggag aatgccggga tgcctttctg gctgtgagct gccagagccg
    11521 ctgccaggta aacagcgccg ggacgttcag tgccgttaaa gccccaaatg gctttcgggc
    11581 gggttggatc catgtcgatg gtttcactgc catagcacca gcaaggcgtt accgtaatgg
    11641 tgaggcctac attctggctg ctgaattttt cttcgcaagc agcggcttca gccatacccg
    11701 cgatacaggt atcggaaatg acacactcga cggcagctcc gcaggcatgg cgcagtttct
    11761 cggtcagcag tgcggccgta gctttcgcca tattcattgt ttgttcttca agcgactcac
    11821 gaacacccat gcgacgaccg tcaataaccg ggcggatacc aattttcggt aagctgattt
    11881 tgttcattac agattcctca ctttattcgg aaaatatgtt cagttagttg ccgtttgaga
    11941 acggaaacgg gcaaagataa agatgaccgc gaagcagagt gcggggatca gttcagcagt
    12001 ggggatgttg cccgccgcgt cactgacaaa acccatgacc ggagtgacaa taccgccgcc
    12061 aatgatggtc ataacgatga aggacgaacc gtatttggtg tcctggccga gattcttaat
    12121 gcccagcgag aagattgttg ggtactgaat cgacataaag gcgctgcata aagtcagggc
    12181 tattaagccc acatgactgc cagcgaaggc tgagatcagg cacagtgcca tagcgattaa
    12241 tgcgtaggcg gccaggactt tgtgtggtgc gaagcgactg atgagccagg taccggtgaa
    12301 acgaccaata aagaagcaca ccatggttcc ggttaaatag ttagcggcaa agcctgcagt
    12361 catgcctgga atttcttcta cagcgtagcg aatcaaatag ctccagcagg ccgtttgtgc
    12421 gccaacgtag cagaattgcg ctaatactgc ccagcgccag tggcgaatac gcgccaggcg
    12481 agaaagcgat gcggagaacg atccttgttt ggcgtcactg tgattatcac tctgcaatgc
    12541 cgggaatttc gtcagcatga tcagcagggc gaccagtaac acgatagcca cgatgatcat
    12601 ataaggtgtc tgtaccgata ataccaggct gtgtttatac gcactcaatt gctctggaga
    12661 cattttatcg agaacgtctt gcgattgatg tggcacgtta gacaaaataa gactttgccc
    12721 aaagacaacc gcgataattg cgccaaatga gttaaatgtt tgcgcaagat ttaagcggaa
    12781 gtgaccactg ctttccggcc ctaataccgt aacaaaaggg tttgcggcag tttccagaca
    12841 acctaatccg gctgcaataa taaataggcc aactaaaaat aaggtgtagt tcattatttc
    12901 tgcggcgggc cagaataatg cagcacccaa ggcatataaa aataatccgg taataattcc
    12961 tgctttataa ctgagttttt tcatcaatat cccagcagga attgggataa tgaaataacc
    13021 aaagtaaaag gccgattgga tcaggccagc ctggaaattt gtcagtgtaa aagcctgctg
    13081 gaattgaggt aataaaatgt cgttaaggtt attggctacc gcccaaagaa aaaacagtga
    13141 gcacagcagc gcgaatggaa taatgtaact tctgctttgc cctgcatctt tatctaccgc
    13201 acggtaactc tgcgtttgta ttgatgtgtt tcccatagca tcctcttagg ttcagaagct
    13261 taagcacgta atcggttatg ttttcaaaag tgcaaaacta gcgagttcgg gcttcatatc
    13321 agtgaatatt tgcctgtctg aaatgaactc atggattcat tttcaaataa aaaacgctct
    13381 gtaatatgac ggcggtcaca cttattagct ggaaggtgac tttat
//
LOCUS       DBFIFM010000125        13366 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.125, whole genome shotgun sequence.
ACCESSION   DBFIFM010000125 DBFIFM010000000
VERSION     DBFIFM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.125"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..401)
                     /gene="yhaJ"
                     /locus_tag="N7C74_003149"
     CDS             complement(<1..401)
                     /gene="yhaJ"
                     /locus_tag="N7C74_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HGU2432970.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVL"
     gene            506..1207
                     /locus_tag="N7C74_003150"
     CDS             506..1207
                     /locus_tag="N7C74_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HGU2432971.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQHLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRTYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1230..1394
                     /gene="yhaL"
                     /locus_tag="N7C74_003151"
     CDS             1230..1394
                     /gene="yhaL"
                     /locus_tag="N7C74_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HGU2432972.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(1528..2838)
                     /gene="cyuA"
                     /locus_tag="N7C74_003152"
     CDS             complement(1528..2838)
                     /gene="cyuA"
                     /locus_tag="N7C74_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HGU2432973.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQQACVAEGEQESPLSVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(2866..4197)
                     /locus_tag="N7C74_003153"
     CDS             complement(2866..4197)
                     /locus_tag="N7C74_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU2432974.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFIIVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGLVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(4472..5836)
                     /gene="tdcG"
                     /locus_tag="N7C74_003154"
     CDS             complement(4472..5836)
                     /gene="tdcG"
                     /locus_tag="N7C74_003154"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HGU2432975.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKAQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(5908..6297)
                     /locus_tag="N7C74_003155"
     CDS             complement(5908..6297)
                     /locus_tag="N7C74_003155"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HGU2432976.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6311..8605)
                     /gene="tdcE"
                     /locus_tag="N7C74_003156"
     CDS             complement(6311..8605)
                     /gene="tdcE"
                     /locus_tag="N7C74_003156"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HGU2432977.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDKNGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(8639..9847)
                     /gene="tdcD"
                     /locus_tag="N7C74_003157"
     CDS             complement(8639..9847)
                     /gene="tdcD"
                     /locus_tag="N7C74_003157"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HGU2432978.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSLRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNTPAEFA"
     gene            complement(9873..11204)
                     /gene="tdcC"
                     /locus_tag="N7C74_003158"
     CDS             complement(9873..11204)
                     /gene="tdcC"
                     /locus_tag="N7C74_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HGU2432979.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11226..12215)
                     /gene="tdcB"
                     /locus_tag="N7C74_003159"
     CDS             complement(11226..12215)
                     /gene="tdcB"
                     /locus_tag="N7C74_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HGU2432980.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(12314..13252)
                     /gene="tdcA"
                     /locus_tag="N7C74_003160"
     CDS             complement(12314..13252)
                     /gene="tdcA"
                     /locus_tag="N7C74_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HGU2432981.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3480 a         3583 c         3149 g         3154 t
ORIGIN      
        1 agcacttctg tgatgattgc cagttgggta ttggcttttg ccgccagttt gtcgattaac
       61 gggaaaaagg caggtgtcgg taccagcgct tcggtcacaa tggtgagatg cgtttcccaa
      121 ccgcgcgcga gagcttccgc atcagtagtc agtttatctg cggcttccag caaaacgcgc
      181 cctcgctcca acaacatcct cccgacattg gtgaatttgg tgcgatggcc cgagcggtca
      241 aacagcacca catccagctc ttcttccagt ttttgcatgg tgtagctaag tgcggaaggc
      301 acgcgtccca gctcatccgc tgccgccgca aaactgcccc ggcgatcgat cgcatccata
      361 acccgtagtg cttccagcgt taatgccctt tctttggcca tttcgttctc attcaggaaa
      421 tttgaacata ccgggcagaa tatctggcta acaatgtagc gtccagcccc ttaccataaa
      481 aggaagtaaa gagaggtcaa gaattatgat tactacccga actgccaggc agtgtggaca
      541 agcagactac ggatggttgc aggcccggta tactttttcc tttggacact acttcgaccc
      601 gaaattgtta ggctatgcct ccctgcgtgt gcttaaccag gaagtgctgg ccccaggtgc
      661 cgcctttcag ccgcgaacct atcccaaagt cgatatttta aatgtgattc tggatggaga
      721 agcagagtat cgcgacagcg aaggcaatca tgttcaggcc agcgccggtg aggcgttgct
      781 gctctctacc cagccgggtg tcagctacag cgaacacaat ctcagcaaag acaaaccgtt
      841 aacacgaatg cagctttggc tggacgcctg cccgcagcga gagaatccgc tgattcaaaa
      901 actggcgctt aatatgggca agcagcattt aatcgcctcg ccagagggtg cgatgggaag
      961 cctgcaatta cgccagcaag tgtggctgca ccatatcgtg ctcgacaaag gcgaaagtgc
     1021 gaatttccag ttgcatgggc cacgcacgta tttgcaatcg attcacggga aatttcatgc
     1081 gcttacgcat catgaagaga aagcggcgct gacctgcggt gatggggcgt ttattcgtga
     1141 cgaggctaac attacgctgg ttgccgattc cccactgcgc gctttgctga tagatttgcc
     1201 tgtctagttg tttttacagg gagatgatga tgagtaaaaa atcggccaaa aagcgccagc
     1261 cagtgaagcc cgtggtggcg aaagaacctg ctcgcaccgc caaaaatttt ggctatgaag
     1321 agatgttgag cgagctggaa gccatcgtcg cggatgctga aacgcgttta gccgaggatg
     1381 aagctaccgc gtaaagacag gttgccggat gcggcgaaaa cgccttatcc ggcctacaaa
     1441 atccaacaac attaacgaat taaacaaccc gtaggccgga taagatgcgc cagcatcgca
     1501 tccggcacga tacccaaaac ctggcgttta tctggccttg ctcgccataa tctcgataat
     1561 ctgccgatcc gtttgctgca tcgaatggct tgctaacgca cacaggttgg caatcgactg
     1621 ctcaacatca tgcgccacga tcccttcatt gccggtcacg gcggtatcat ccagcgccat
     1681 taacaccgct ttccacgcag ccgaagcact ggtcgaaacc ttcatcgcgc agctgttcga
     1741 cgcaccatcg caaatcatgc cgctgacatc gccgatcata ctgctgatcg ccatcgagat
     1801 ggtttcataa cgcccatcca ccagccatgc catcccggcg gcggccccca ttgctgcggt
     1861 cgttgcggca cacagcgcag acaaacgcgg taactggtta tggatgtaaa ttgcgctcaa
     1921 atgagaaagc atcagcgcac gcgccagccg ttcatcatcc gctccgaagt gttctgctac
     1981 caccaccacg ggcattgttg cggtgatccc ctggttaccc gagccggagt tactcattgc
     2041 cggaagcgtg gcgccgccca tacgcgcatc ggatgccgcg ctggtacgaa tcacaatgga
     2101 tgaagagaga tctttcgcca gcaaaccgcg ctcgcactgt ttttccagcg tcgcgccaat
     2161 atgcagcccc cacttaccgc tcaaaccttc ctgcgataac gcacaattta gcttcgcgga
     2221 atcgagaata aagcggatcg ccgcaaacgg gacttcattg acgaacttca ggatctcagc
     2281 cagcgtcgtt ctggaaagca ccgatagcgg agactcttgc tcgccctctg ccacacacgc
     2341 ctgctgggta aacaccacac catcgtgcgt ctcaatatgc acaatgttgg tatgcccgcc
     2401 gacgatagtg acacacgccc acttctcacc gttccagact ttggcgcgtg agaagaggat
     2461 ttcattgcaa ggttcctgga tcttaacgga gactttcccc gccgccagca gtgctttggc
     2521 atcggcaatt gcctgcgcag ttgcgtcttt cagcacttcc agcccggcgt tggcatttcc
     2581 acctaacgcc cccagcgccg ccgcaatcgg cagccccacc attcccgtgc cgggaacggt
     2641 gacgcccaga ccgttcttca tcagatttgg cgaaacccag gcttctacac gttcaaccgg
     2701 accttccagt tctgccgcag caaccgccgc cgccagcgcc agtgaaatcg gttcagtaca
     2761 tcccagcgcc ggttttactt cctcctgaac ggcgaggatg taacgctgcc ataacggatt
     2821 taaagtcgaa tcaaacatat taaaaacctt aaaatttcag gtaaatcaag aaaatgccag
     2881 gaacggagaa acacaaagca acaaaccagt gacgataatc aggtacagag acatcccttt
     2941 gtatttgtgc aatgccggta ctttgtaaac cagccacgcc gggatcaggc accctaccaa
     3001 gccgaaaatc gggctacaga tagaggtgaa gctcaacacc ggtgcgttca gtacgatggc
     3061 gctccaggcc agcaaaatgg cgaaaatcat gatgccgcgc tgaacaagat tttcgttaat
     3121 cttctcggca ggcatcttgc gacgcaggat gttcattacg atcccttgcg ttgcttcgcg
     3181 aaaacctaaa tagacgccaa agaatgcagt catgacagca aagatattga gaatgacgct
     3241 gaccactttc acccacgctg cgccgtcacc gctaataaac tgtgcggcaa tcgccagcgc
     3301 ggaaatattc tgctcgtagg cttttaccgc ttcgtcatgt cccatcgcca gcgtgaacga
     3361 cacggcgtag aaaaagacga tgataaacaa aatgccaaac gcgatattca ttgcccgcaa
     3421 tgctttatgc cgcgctactt caatcgattt ttcccgcgag cgataagaga tcaccatcgg
     3481 gcttaacgtc tggataaaca gaatcgacgt cagggtaaac ggcagcgtaa taatagcgtt
     3541 tttcaccagc agccccagcg gcggtagcga accgacgtta tacagatgcc acattcccac
     3601 catcgacacg cccagcgccg cgaccaccag cagcttggtc agcaccatgc cagtcgaaat
     3661 tttgaataac aatttctcgc cgcgtgaaga gatcgccacc agaatgcaaa tcagtaccag
     3721 accataaaag ggactgtctg acagcaaccc ttccgtcacg ccgaaggtat gcaggtagga
     3781 agcactatcg ttggtgatgg cggtggaata gacgaacatc caaatcacca gcattacgaa
     3841 atagagtgca cctaacagga tgccccagtt tttacctaaa taaccgctaa tgacgctcgg
     3901 gtaatcttta cattctggtg attctgccag cgtattaata aacaaccgct gaaacagata
     3961 cattgccggg taaccaatca ccgatgagag caaaaatacc cacaatccca tcaaaccgac
     4021 ctgcaccggg agaaaaacaa tccccgcgcc aatcgccatc ccgatactca taatcaccca
     4081 gccggtgtca gtactgtcga atttgatcgc ttctcgccac tcgctctcac tcattccagc
     4141 acgacccgcc ggggtcgaag cgtctgcaat gacgccttta ttcgatgcaa tttccataat
     4201 ttctcgctca atattttgta gggcttatta tttttttccg agccgcatca aggcgatatg
     4261 cggtctacgt ttttgcaggc gagttattag aataaagaaa tgatacgcgc gaggtcagag
     4321 aaaatcttca caatcaagac tttgaaataa cggagatgga taagaatttt ctactaaatt
     4381 aatcgcaaga gaatattcac tctctatttg cggcagatca caaaaaaaag gtgcacattt
     4441 gtgcacccaa ggatgaaagc tgacagcaat gtcagccgca gaccacttta atggccagtc
     4501 cgccgcgtga tgtttcgcgg tatttatcgt tcatatcttt gccggtttca tacatcgtct
     4561 cgatcacttt atcgagtgaa acacgcggtg ccgaggtgcg gcgcatcgcc atccgcgcgg
     4621 cgtttactgc tttcacggca ttaatggcat tacgttcaat gcacgggatt tgtacctgtc
     4681 cggcaaccgg atcgcaggtc agtccaaggt tatgctccat cgcgatttcc gccgcattgc
     4741 atacctgcgc cggactgccg cccagtagtt cagttaaccc tgccgccgcc attgaacagg
     4801 ccacgccaat ctccccctga cagccgacct ccgcgccaga gatggaggcg ttcattttat
     4861 acagcgcgcc aatagccccg gctgcaagaa aatagcgggc aattgaccgc tcgtttaccg
     4921 gacgacggaa cttatcgtaa taagccagca ctgccggaat aatgccgcac gcaccgttag
     4981 tcggtgccgt taccacgcgc ccgccagctg cgttttcttc actaaccgcc agcgcgtaca
     5041 tgttgatcca gtcgatgaca ttcatcggat cgttagagat gttatcgctg gaaaccagct
     5101 gacgacgcag cgctacggca cggcgcggca cattgagcgg accaggcagc acgccttcag
     5161 tgttcatccc gcgttcaata ccgtcatgca tcacttgcca gatacgggca aaaccggcgt
     5221 caatttccgc tttactgcgc agcgctagct cgttgtgcat catcaggcca gaaatagaca
     5281 ggccgttgta atcacacatt ttcagcagtt cacctgctga gtggaaatcg taaggtacgg
     5341 gtgtttcgac atcgtgcgac aggccgaagt gttcttcttc gacaataaac ccgccgccga
     5401 cagagtaata ggttttactt aatagctctt cctgtgcctt ccaggcagtg atccgcattc
     5461 cgttctcatg gcgaggcaac atttcgggat ggaagataat gttctttgct acaggaaaat
     5521 caacaatatg cgcaccagat gccactggca gccgcccgct gcgcgttact aactctataa
     5581 atgcagggat ctcatcaatg acaacatcct gcggactgtt tcctgccagt cccatgatga
     5641 tggcgacatc cgtggcatgg cctttgcccg tcagtgacaa cgacccgtac agatcgacca
     5701 caatatggct cgtcgcggtt aataagccgc tactttccag ccgatcaata aaactttttc
     5761 cggcattcat tggccccacg gtatgcgaac tggagggacc aatcccaatt ttgaaaatat
     5821 cgaatgcact aatcatatcc acaccctcgg attgccgttc agtgaagtgg agcggaacga
     5881 ccttacgacc gtcccgctca cgaggcttta cgcactacgt actgcgatag cttcaatttc
     5941 cagcttcaca tctttcggca aacgcgcgac ctgtacacag ctccgggtcg ggtaggtcgc
     6001 ctgatgctca tcgaagaatt gcttatagac ttcgttgatg gtggcaaaat cattcagatc
     6061 ggtgatgaac acggtcatct tgatgatatc gcccacgctc agcccggcag caaccacgat
     6121 cgctttgacg ttttcgaggc ttaaacgcgc ctgatcttgc acatcagccg ggatctcacc
     6181 ggtctgtggg caaaccggaa tttgaccgga ggtgaagacc atgctgccta aatcaacgcc
     6241 ttgaacataa gggccgattg cgcctggggc acgttgcgtt tcgataatct ttttcataca
     6301 tcctccggcg tcagagcgcc tgggtaaagg tacgtgaaat aacatcctgc tgctgttcac
     6361 gggtcagtgc gttgaagcgc acggcgtagc cagagacacg gattgtcagg ttaggatatt
     6421 tttccgggtg ctcgatggca tccagcagca tttcccgatt cattacgttg acgttgaggt
     6481 gttgaccgcc ttcgacatcc gcttcgtggt ggaaataccc atccagcagg ccgacaaggt
     6541 tggttttacg tactggatct tctttgccca gcgccgcagg gacgattgag aaggtgtacg
     6601 agatcccatc tttggcgtag gtgaacggca gtttcgccac cgacgtcaat gaggccacgg
     6661 cacctttgcg gtcacgacca tgcatcgggt tagcgcccgg cgcgaacggt gttccggcgc
     6721 gacgaccgtc cggcgtgtta ccggttttct ggccgtacac cacgttagaa gtgatagtca
     6781 gaatcgactg ggtagggacg gcgttgcgat aggttggcag cgctttaatt ttcttcataa
     6841 aacgttcaac caggtcgcag gcaatgctgt ctacgcgctc gtcgttgttg ccgtactgcg
     6901 gatattcacc gtcgatttca aagtccaccg ccaggccgtt tttgtcacgg attggtttca
     6961 cgcgggcata tttgatcgca gacagggagt ccgttgccac cgacaggccc gcgatgccgc
     7021 acgccatggt gcgatagaca tcacgatcgt gcagcgccat cagcgaagct tcgtagctgt
     7081 acttgtcgtg catgtagtga atgatattca gcgcgctgat gtactgcacc gccagccagt
     7141 ccatgaagtg atcgaggctg tccatcactt tgtcgtaatc cagcacatcg tccatcaacg
     7201 gtgccgtttt cggcccgacc tgaatcttca gcttctcgtc caccccgccg ttaattgcgt
     7261 acagcagtgt tttcgccagg ttagcgcgtg caccaaagaa ctgcatttgc ttaccaatca
     7321 ccattgggct gacgcagcag gcaatcgcgt aatcgtcgct gttgaagtca gtacgcatca
     7381 gatcgtcgtt ttcatactgc aaggaagagg tgacgatcga cacctgcgcg gcatattttt
     7441 tgaaggcaat cggtaattct tccgaccaaa gaatggtcag gttaggttcc ggtgcaggcc
     7501 ccatagtgtg cagggtgtgc aaatagcgga aggagttttt ggtcaccagc gtacgaccgt
     7561 ccagccccat cccgccgatc acttccgtcg cccagattgg gtcgccggag aacagcgaat
     7621 caaattccgg cgtacgcaag aagcgcacca tacggatctt catgatgaag tgatcgatca
     7681 gttcctgtgc ctgctgctca ttgagtacgc cagctttaaa gtcgcgctca atgtagatat
     7741 ccaggaacga tgccgtgcgg cccagcgaca tcgcgccgcc gttctgtgat ttcactgccg
     7801 ccagataagc gaagtagagc cactgcaccg cttcctgcgc attctgcgcc gggcgagaga
     7861 tatcaaagcc atatttcgcc gccatttcct gaatctgcaa cagcgcatga cgatgctctg
     7921 ccagctcctc acgcagacgg atggtggctt ccagatcctc gcctttttcc agacgagact
     7981 ggagatcggc aaattgcagt tcgcgttcac gcaccagata actgatgcca tacagcgcta
     8041 cgcggcgata gtcaccgata atgcgcccac ggccatagcc atctggtaaa ccggtcagca
     8101 cgccagattt acggcagcgc agcatatccg gtgagtaaac atcaaatacg ccctggttat
     8161 gggttttacg cagatcggta aacagatatt caaattcact gtccatttca cgaccatagg
     8221 cgtggaatga acttttaatc atattaatgc caccgaacgg gtgtaacgca cgtttcaacg
     8281 gcgcatccgt ttgcaggcca acaatttttt ccagcggctg gttaatatat cccgcatcat
     8341 gagcggtaat tgtggtggca atattggtat cgaaatcaac cggcgcgtgg gttgcgtttt
     8401 caatacgaat gccttccatc accttttccc acaattccgt ggtggctggc gtcgcttcag
     8461 cgaggaaaga ttcatcgcct tcatacggtg tatagttatg ttgaataaaa tcgcggacat
     8521 taatttcgtt tttccagtcc gtacctttaa agccaagcca tgcgtcggcg tacagcttat
     8581 cgctggtatc aatatctacc ttcatgaaaa ataatctctc tacaatactt caactaaatt
     8641 atgcaaattc tgcgggtgtg ttaactttgc ctaaatgaat ggcatccaaa gcaatcattt
     8701 tttcttcgtt ggtcggaatt acggcgcaaa tgacacgcgc attttcactg gaaacaattc
     8761 gctcgccaaa agagttagat cgattattca tttctgtatc aatctctacg cctaatacag
     8821 ccaaatgttc catgaccaga cgacgaatta aacttgaatt ctctcctatt ccgccggtga
     8881 atataattcc atccaggcga cgtaatgaag ctgcgtgtcc ggcaatatga cgggcaattc
     8941 ggtgaacaaa ggttttaatt gccagctgcg cgcgttcgtg accttcatgc caggcttttt
     9001 ccagaacacg taaatccgaa gaaagaccgg aaatacctaa taatcccgac tctttattca
     9061 ctacgcgttc caggtcaccc aggctctggt tggtttggct ggcgacccag gacatcgcac
     9121 caaagtcgac atcgccactg cgggtaccca tcatcaagcc ttccagcggc gtcattccca
     9181 ttgaggtgtc aacactctga ccgttgcgaa ctgcgcagat tgacgcgcca ttgccaagat
     9241 gcgccacaac caggccggag tcatcttccg ccagattcag caaagaatgt gcgcgcaggg
     9301 aaacatagcg gtgcgacgtg ccgtggaaac cataacggcg tacacctaac tcttcataat
     9361 atttccacgg caggccgtat aaataagctt ccggagccat cgtctggtgg aaactggtat
     9421 caaataccgc cacctgagtt acgcccggaa ataattgctg cgccgattca ataccactta
     9481 aattggcgta attatgcagg ggtgccagtg gagaaacgcg acggatatta tcaatgactt
     9541 catcggtaat aatggcggac tcggtaaaaa tactgccgcc gtgagcgatg cggtggccaa
     9601 ttaaggccac gctgtcattt aaattccgtt tttccagttc aaatgcaatt gccttcaatg
     9661 caccttcgta gctgtggtga gccagcggtg ctggctctcc cccatttacg gataagaatg
     9721 cattttccga gttaataccg tcggcaatac ctgacattaa tacttcacag tcgctggcat
     9781 cgagtacgga aaacttaatc gaagacgaac cacagttaat aaccaaaaca accggaaatt
     9841 cattcatctc ttttctcatc ctgagttacg gattaaaaca gtttatatac gatgttcagg
     9901 atggtcagca gaccaatcac ggtaacaaac acgttatcca gacgaccacg gtatttcgcc
     9961 agagacggcg ctttacggat ggcatacatc ggcaacaggc acagcaggga tgcgataatc
    10021 ggtgcgccca tggcttcaat caggtcgagg atgttcgggt tggcgtaggc aacaacccag
    10081 gtggagccca tgatgaagat catgctgata gtgttcagtt tacccagcga tactttagtt
    10141 ttgtcgcctt tataaccaaa cttcaggacc aggccattca gaccttccag cgttcccaga
    10201 tagtgaccga agaaagattt gaagatagcc acgagtgcga tgatggaagc cgcatattcc
    10261 agtgtaatcg cgaacgttgt tttggtaccg gtcatggacg caaagtggtt agccagataa
    10321 gaaagcactg gaatattctg cgctttggct tccgccatgt tggccggaga cagcgtaaac
    10381 aggcagctaa aggcaaagaa catcaccact gcaaccatca gcatgctggc acgagaaatg
    10441 atttgggaac atttacgttc ggtgaagtcg cgaccgaagt ctttctcata ctcttcacgt
    10501 ttagaaacca cgaaggaaga gacgattggc gagaagttaa aggagaaaac catgatggaa
    10561 atccccagcc agacagtgat caggataccg tcatgaccgg ttaacgacag cgaaccgagg
    10621 tcaacctggt cgataactgc agagttccag taagggatca gcgacaaaga aatcagcacc
    10681 aggctggcga taaacggcca taccaggtag ctcatcactt taaccatcag atccttacca
    10741 aaccagatga cgaaagccat cagcagcaac aggaacagcg ccacaaagcc gcgattcagc
    10801 ggtgcaaagc cgagctggtt ttcccagaac gtcataaagg tattggtaat agtaacgcca
    10861 taaatccaca gcagtgggca aatcgcgaag aagtacagga acgtgataac cacgccgcca
    10921 gttttaccaa aatgctcttc caccgtttcc gtaatgttgc cggaagggtt agagccagaa
    10981 agacacagac gcgccagcgc ccggtggcaa taaaatgcga tggggtatgc caataccaac
    11041 atcagaagaa tcgggatcag tccgccaaaa cctgcgcgga tagggaagaa cagcaccccg
    11101 gcgccgattg ccgtaccaaa caagcctaac gtccatgtgg tatctgattt acgccaggac
    11161 gattgttttg tctggctgga tacaatgcta tctgaagtac tcatatccta tcctcaacga
    11221 attaattaag cgtcaacgaa accggtgatt tgagagacgc gagaaagatc gatattgccg
    11281 ccggaaataa tactgacggt ttttctgttt tgaatatatt ggtctaattt accgcttaat
    11341 aatgcagcac atgccagagc gcctgcgcct tcggtgacga ctttattgcg ctgaattaag
    11401 gcaatcatac tgtttctgat ttcgtcttcg ctgaccagca cgatgtcatc gactaattca
    11461 cgaacgattt cgtaagttaa attacccggg cgggagacat cacaaccatc cgccagggtg
    11521 ccggtagttc ggtgcgtggt tatttctccg gagtggaaag aagccgccat gccgtgaacg
    11581 ttttcagact gtacgccaat aacacgaatg gtcgggttaa tagatttaat tgccaccgca
    11641 ataccagcaa ttaaaccgcc accaccaatt ggcacaatca cgttatcgac atcatagaga
    11701 tcttccataa tttccaggcc aatcgttccc tgaccagcaa tcactttcgg atcatcgtaa
    11761 ggtgggataa aaatacggcc ttccatttcg acaatttcgc tcactttagc gatagtgtcg
    11821 ttgaagttat caccatgcag aacgacttct gcggagtagt cgcacgttgc cgctactttg
    11881 gatttcggcg cgccttttgg catcaccact ttaccgtcga tacccagcat cgcgcaggag
    11941 agcgaaaccc cttgcgcatg gttgcccgca gaacaggcca ccacgccttt gcgtttttcc
    12001 gcatcagtca gtgaacttaa tttattaaat gcgccacgaa ttttaaatga acccgtacgc
    12061 tgcatatttt caaacttcag gaatatttca cctttgcaac gttcactaaa atagttggag
    12121 cgaggcatgc ctgttttata aattcgccca gccagtcgtt gtttcgcttc aataatgtca
    12181 tcaatagcaa ccggcagatc gtatgtaata tgcattataa aacctcttcg cctgaattaa
    12241 atatgtaggt aaccgtaacc gacacctgca agacagacag gtggattatt tatgtttaaa
    12301 taaaaacaaa taactaacca acttctatta attgccttcg tctacaccca ttataagatg
    12361 aatactcttt ggctaattcc accaaaaccg atgctgcttt tttaatacga taatttttcg
    12421 accataccgc ggcatattgt gccacaggta atgtttcttc aaccggaata gtaataaatt
    12481 gattagaacc aaaaggagac gtcatatcac aaggaattac agttaagaaa tcagcattga
    12541 gaacaagatt ataaattgtc acgactgagt cggttttaac gatgttttca atactgatgc
    12601 catttctttg taacgtagta agcagttcgc tgtagtaccc catattagtt tgtggcaaca
    12661 cccactgttc gttcttcaac gactccagcg tggtggtgcc ggtgcatgtt cgggacttac
    12721 tggctaccag cacaaactcg gactcgaaca gcggctcaac atgtaaatcc tgaagcttca
    12781 tttctgcgct taacgtacca atcgcaaaat caagtcgacc gtcgcggatt gccggtaaga
    12841 acgaagacag ttgcgcttca tacatagaaa cctgcgcttt cgggaacacc tctttgaact
    12901 tgttgatcat ccctgacata aaagtaaaac caatcaatga aggaaaacca aatgagactt
    12961 ccaccaccgc ctcagaagac ataccgctta tctcattaac catatttttc atttcacggg
    13021 taatggattc ggaacgagag agtaacaatt gaccggcagg tgttaatgtt actccggtat
    13081 ttttccgcac cactaattcc acaccaaaat aatcttcaat atcgttaatg attttactga
    13141 cggccggttg agttaaccct aattcttttg cagccgagcc gatagaacca cttctaatga
    13201 cttcctgaaa gactaccagg tgctgcgttt tcggaagaag aatagtgctc ataacgacct
    13261 acgttaatta cctcattgac ggcatgaagt gtatcaaaat gaaatgaaca ggatatgtgc
    13321 gaccactcac aaattaactt tcaatacttt ccagggtatc gtcatg
//
LOCUS       DBFIFM010000126        13317 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.126, whole genome shotgun sequence.
ACCESSION   DBFIFM010000126 DBFIFM010000000
VERSION     DBFIFM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.126"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            125..367
                     /locus_tag="N7C74_003161"
     CDS             125..367
                     /locus_tag="N7C74_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HGU2432982.1"
                     /translation="MNSLAGIDMGRIILDLSNEVIKQLDDLEVQRNLPRTELLREAVD
                     QYLVNQSQTVRTSAFGIWQGCEEDGVEYQRKLREEW"
     gene            complement(697..1626)
                     /gene="fdhE"
                     /locus_tag="N7C74_003162"
     CDS             complement(697..1626)
                     /gene="fdhE"
                     /locus_tag="N7C74_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HGU2432983.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1623..2258)
                     /gene="fdoI"
                     /locus_tag="N7C74_003163"
     CDS             complement(1623..2258)
                     /gene="fdoI"
                     /locus_tag="N7C74_003163"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HGU2432984.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2255..3157)
                     /gene="fdoH"
                     /locus_tag="N7C74_003164"
     CDS             complement(2255..3157)
                     /gene="fdoH"
                     /locus_tag="N7C74_003164"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HGU2432985.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNHADEEENNLHEEKDEERK"
     gene            complement(3170..6220)
                     /gene="fdnG"
                     /locus_tag="N7C74_003165"
     CDS             complement(3170..6220)
                     /gene="fdnG"
                     /locus_tag="N7C74_003165"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5633..5635),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HGU2432986.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQGNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVISCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6414..7247
                     /gene="fdhD"
                     /locus_tag="N7C74_003166"
     CDS             6414..7247
                     /gene="fdhD"
                     /locus_tag="N7C74_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HGU2432987.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            7401..8456
                     /locus_tag="N7C74_003167"
     CDS             7401..8456
                     /locus_tag="N7C74_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HGU2432988.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPLPANSQPAAPAP
                     EAKPTEAPVAKADAKPETPAQPLVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYADW
                     LKDFKQGPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAV
                     ALGNTINEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKR
                     QLAELKNIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQA
                     KEADKGGMNFSFINSADQYQLETKKYVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFP
                     RALREYNEMVDDYNSLR"
     gene            complement(8506..10254)
                     /locus_tag="N7C74_003168"
     CDS             complement(8506..10254)
                     /locus_tag="N7C74_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HGU2432989.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHNGLFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGLASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(10254..11324)
                     /locus_tag="N7C74_003169"
     CDS             complement(10254..11324)
                     /locus_tag="N7C74_003169"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU2432990.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAKKRGAEIGDFISPEAN
                     FACWGEDKIVGKALDNRIGCAMMAELLQTVNNPGITLYGVGSVEEEVGLRGAQTSAEH
                     TKPDVVIVLDTAVAGDVPGIDNIKYPLKLGNGPGLMLFDKRYLPNQKLVAALKNCAAH
                     NGLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            complement(11314..12753)
                     /locus_tag="N7C74_003170"
     CDS             complement(11314..12753)
                     /locus_tag="N7C74_003170"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HGU2432991.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAIPYAL
                     AAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLAIGLA
                     LNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            complement(12764..13210)
                     /locus_tag="N7C74_003171"
     CDS             complement(12764..13210)
                     /locus_tag="N7C74_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HGU2432992.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSTCVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
BASE COUNT         3209 a         3434 c         3475 g         3199 t
ORIGIN      
        1 tgttacatca tatctggaaa acgcctcgca aaaataaaaa atgatgcgta aatgagcttt
       61 ctttaaagat tcgacaaggg tacttttctc tccatagata tcaaatgata ttggtcaaat
      121 atttatgaac tctctggcag gtattgatat gggcagaatt atactcgatt tatcgaatga
      181 ggtgattaag caacttgatg atcttgaggt gcagcgtaat cttcctcgta cggagctatt
      241 acgggaagcg gtagatcaat acttggtaaa tcaatcgcaa acagtaagaa ccagtgcttt
      301 tggcatatgg caagggtgtg aggaagatgg tgtcgaatac cagcgtaagc tgcgcgagga
      361 atggtaatgg gaaaaaatga tatcaatcag attgctgaca acgtgcgcgt tgttcatgcc
      421 ggatgcggcg tgaacgcctt acccggccta caaagtcgta taaattcaat gtattgcagt
      481 ttattggtag gcctaataag cgcggcgcat caggcaatct tacgcttatc atcagtctca
      541 tacccataac cttattgatg tgggggtggg taaattgagg caaaacaggc actggcggca
      601 tagcttgagg tggtaacgcg gaatacgaga gatggtgtcg tcacacccta tgagctgtaa
      661 tccgggcgac cagcgccacc cggaaaacgt cagagattac tccccttcac ccggaaacag
      721 gaacgggttg atggaactgc gggcatagcc ttcttgctcc attcgcgcgt ccagtaccag
      781 agaggcgagg tcatctgcca cggcttcaac tttcggttct ttttcctgat agagaatttt
      841 caggtaagtg ccgcagtcat cgcagctttc ggctttaatc gcggcctgtt cgtcatccag
      901 cgaccagtaa tgcagtttgc cgctctgctc gcagttgctg cattttacgc gcacaacgtg
      961 ccattcagtt tcacacaggt tgcagtgcag gtaacgtagc ccctgagtgg tgccaatttg
     1021 caccatgctg gacaccggca tagaaccaca aaccgggcaa tattgacgtt gctcgccgta
     1081 ttcagcgcgg gctttgccgg ggatcagatt ggccatctgc gcccagtaga gcgacagtgc
     1141 agcccagata aacggcgctt tatcgctgct gacggacgag aaatcagagg caaacagtgc
     1201 gctggccata tcttccagct cctgagtcga tgccttctcc agattctcaa tcactgccag
     1261 cgccgggccg ctcatttcag gtttcagctc agcaatcagc gccatcagca gcttttgcca
     1321 gtgcttatca cgcggcagaa cgtgaatatc cagcgggggc ttgccttgtg cgctggcttc
     1381 tttaatgcgc gcagtcagat ccatctccag cggatggtcg tacagcacca cttcctgggc
     1441 gtgggcgata agcgcagcaa agcgcaggta atcacccagc ggattatttt ctgccagctc
     1501 gcgcagacgc tcggcgcggc ggttgtataa attcttgagc cgagggaaca ataacggcgg
     1561 aatcatatcc gccgtacgtt tctcgctcga acccagctca tcttgcggga ttatgcgaat
     1621 actcattcag ctttcttttc cgttgtcttg cggacctcac ggtaccagcg cgggtgatgt
     1681 ttcttcgccc aagcgctggt aacccatcct tccaccatgg cggtaatcgt gcctttcacc
     1741 caaagggcgg cgtagatatg caccatgata accacaatta acgccactgc ggcaaatgaa
     1801 tgcagcatta acgcgaatcg gatcaccggg attgagaaag caggcgcaaa ataaggacgc
     1861 cagataatca cgccgctcac cagcagcaga accaggaaaa taatcgccgc ccagaaaacg
     1921 catttctgac cgaagttata acgcccggtg tcacctactt cctcgttgac gacgatctta
     1981 cgaatattct tcgcccaaaa gatatcatcc cgattgatta ggttgtgatg ccagtaacgg
     2041 aaaaacatga tgatgaacga ggcaaacata accacgccga caaacgggtg cagaattcgc
     2101 gccagctgcg gtgtgcccat gatttgcatc aaccagttga aggacgggaa caaaaagccc
     2161 agcccgctca ccgccgccag gatgaagcag aaggcggtga tccagtggtt gatacgttcc
     2221 ggcgcggtgt agcgcacgat ggtgtcacgt cgtttcattt gcgctcctcg tctttctctt
     2281 cgtgcagatt attctcttcc tcatccgcat ggttcggacc gacacccacg tagtggaaga
     2341 tactggctgc gaaggtagcc gcaaagccaa cagctgcgag cggtttccag atgcctttcc
     2401 agaatttcac ggtttcgctg atttccgggt tctccggcaa gccatgatac agatttggct
     2461 tgtcagcatg gtgcagcacg tacataacgt gtgtaccacc gacgcctgcc ggatcgtaca
     2521 ggcccgcatt gtcgtaaccg cgggttttca gctccgccac gcgctcgctc gccagcgttt
     2581 tcatcgactc tttcgtaccg aagtgaatcg cgcccgttgg gcaggtcttc acgcaggctg
     2641 gttcttgccc aaccaccacg cggtcaacgc acagcgtaca tttgtagacg cggttgtctt
     2701 ccgggttgag gcgcggaatg tcgaacggac agcccgcaat gcaataaccg cagccaatgc
     2761 actgctcgga ctggaagtcg acgataccgt tggcatactg aataattgca ccttccgccg
     2821 ggcacgcttt cagacagcct ggatcggaac agtgcataca gccgtctttg cggatcagcc
     2881 attccagttt gtcgttctgc tccacttccg agaagcgcat caccgtccac gatttggcgc
     2941 ttaaatcatt ggggttgtcg tacaccccaa tgttattgcc gacggtatcg cgaatgtcgt
     3001 tccactctga acacgccacc tgacaggctt tacagccgat acaggtggtg acgtcgatga
     3061 gtttcgccac ttcttcctgg aagtcccgcg cctgaggcgc gggggtcaga ccgttagtcg
     3121 cggaacgacg aatgatatct tgagattgat aagccatatg tcgtctccgt tacacctttt
     3181 ccacattcac aaggaaggac ttaaactccg gcgtctgcgt gttcgcatca ccgacgaatg
     3241 gcgtcaacgt attggcaata aagccttttt tcgcaacacc ttcatagccc cagtgaatag
     3301 gaataccgat ggtatcgata tctttgccgt tcgctttcag cgtgcgaata cgtttggtca
     3361 ccaccgcttt ggctttgata tagccacggt tggaggagac tttcacggta tcgccctggg
     3421 caatgccaag tttattcgcc agcgactccc cgatttccac aaactgctct ggttgcaaaa
     3481 tcgcgttcaa cagcgcgtgt ttggtccagt agtggaagtg ctcggtcaga cgataggtgg
     3541 ttccgacata cgggaactta tcggctttac ccaatgcttc ggcgtcgtct ttaaagatac
     3601 gtgcagccgg gttcgagata acgtttggat gcagcgggtt agttcccagc ggcgtttcaa
     3661 acggctcgta gtgttccggg aacggacctt ccgccatctt atcgagggca aacagacgcc
     3721 ccatgccttc ctgctgcatg ataaacggcc cgacgccgct acccggaggc gctgcgctgt
     3781 agtccggaat atcccagccg gtccacttag tgccgtccca tttcagcaac tgacgcttcg
     3841 gatcccacgg gttaccctgc ggatctgcgg aggcgcggtt atacagaatg cggcggttaa
     3901 gcggccatgc ccatgcccaa cccagcgtgt taccgaggcc agacggatcg gcgttatcac
     3961 ggcgtgccat ctggttgcct tccggcgtcc agctaccggc gaaaatccag caaccacagg
     4021 aagttgtacc gtcatcgcgc agttgggcaa acgagctaag ttgttggcct ttcttgacga
     4081 taaccgcacc ggttgccgga tcggtaatat cggccagcgc cttaccgttg ctctccatcg
     4141 ctacttcttc cgacttcggc tcatgcggaa tggcgtagtt ccaggtcatg ttcagcacct
     4201 ggtccgggtt cgcgccaccc tgttcggcat acatcttgcg caagcgcagg aagataccgg
     4261 agaggatctc gccatcagtc agcgcaatcc ccggggcgtc cgcacctttc cagtgccact
     4321 gcaaccagcg acctgagtta acgattgaac cgttctcttc tgcgaagcag gtcgatggca
     4381 gacggaacac ttcggtctgg atcttcgacg agtcaacttc gttcagctca ccgtggttct
     4441 gccagaagtt agaggtttca gtgttcagcg ggtcgatggt gacgaggaac ttcagtttcg
     4501 acagacaact gatcactttg tttttgttcg ggaatgaggc aacagggtta aagccctggc
     4561 agatatagcc attgaccttg ccttctttca tcatctcgaa gtattgcagg acgtcgtagc
     4621 ctttatccca cttcggcaac cagtcaaagc cccagctatt ttctgccgtc gctttatcac
     4681 caaagaaggc cttcatcata gagacgaaga atttcgggta gttgccccag tagtttccct
     4741 ggccttccag cagcggtttt ggcgtgttgg cggtaaggta ggtttgcaga tcggtctgct
     4801 tctcgcttgg cagcgtcatg taacctggca ggctctgcga cagcagcccc aggtccgtca
     4861 gcccctgaat attggagtga ccgcgcaggg cgttaacgcc gccgccagcc atccccatgt
     4921 taccgagcag cagctggatc atcgccatcg tacgaatgtt ttgcgcacca acggagtgtt
     4981 gcgtccagcc gagggcatac aggaacgagg cagttttatc gtgagcactg gtttctgcga
     5041 tgtattcgca gactttcagg aacgcgtctt ttggcgtacc acagatgttt tcaaccacat
     5101 ctggcgtgta gcgggaaacg tgctgtttca gcaagttcca cacgcagcgc ggatgttgca
     5161 gcgtggtatc gcgtttggcg aagccgtttt cgtccagttc ataagtccag gaggatttat
     5221 cgtacttgcg tttttccgcg tcgtagccgg tgaacaggcc atcttcaaag ccgtaatcct
     5281 cacgcacgat caggctggcg ttggtatagg cttcagtgta ttcgcggttg aatttttcat
     5341 tgttcagcag gtacagcaat acgcctgaca ggaaagcaat gtcagtaccg gaacgaatag
     5401 gggcatagta gtcagccacc gcagccgtac gcgtaaagcg aggatcgatc acaatcagct
     5461 tcgcgccatt gtgaattttg gcttccatcg cccagcggaa cccgaccggg tgagcttcag
     5521 cggcgttacc gcccatcacc acgacgaggt tggcgttctt gatgtcgacc cagtggttgg
     5581 tcatcgcacc gcgaccaaat gttggagcaa gacttgctac cgttggtccg tgtcagacac
     5641 gcgcctggtt gtcgaccgcg agcataccca gcgcgcggga gaatttttgc gttaaatagc
     5701 cggtttcgtt actcgacgcg gaagcacaca gcatcccggt ggagagccag cggttaacag
     5761 tcacgccttc ggcgttttgc gcaatgtagt tagcatcgcg gtcttctttc atcagtttgg
     5821 cgatgcgatc aaacgcctct tcccaactga tttgctgcca tttatcagaa cctggcgcac
     5881 ggtattccgg aaacttcaga cggctttcgg agtggatgaa atccaccagg ccagcgcctt
     5941 tcggacaaag tgcaccgcgg ttgaccgggt gatccggatc gccttcgata tggaagatag
     6001 atgctttggc gttttttgct ccgtcaccga ggctgtacat caacagccca cagcctacgg
     6061 aacaataggt gcaggtatta cgggtttcgc gggtgcgcag cagtttatac tgccgggttt
     6121 ccgcgagtgc tacgctgggt gcaaaaccca gtgccgctgc cgtggtgcct gccataccgc
     6181 cagcgcagat cttaaagaac tgccttctgc tgacctgcat ggattgctcc ttgtttcgac
     6241 attgtcacgt cccatttaca ttcgcttgct gcgtgtgcag ggagtgggag ttatttttct
     6301 ttgcggaagg ggccgcaaag gtccagaatt ggctcaattt ccctccatcc aggaagggtt
     6361 tgtaacagaa taccataatg ttggtgtgtg tgttcttatc tggttaagag aaagtgaaaa
     6421 aaacacagca aaaagaaatc gaaaatgtga caaatatcac aggtgttcgt caaattgagt
     6481 tatggcggcg tgatgattta caacacccac ggttagatga ggtcgcggaa gaagttcccg
     6541 ttgcgctggt ctacaacggc atttcgcatg tggtgatgat ggcgtcgccc aaagaccttg
     6601 agtactttgc gctcggtttt tcgctttccg aggggataat agaaagtccg cgcgatatct
     6661 tcggcatgga tgtcgttcct tcctgtaatg gtcttgaagt acaaattgag ctttccagcc
     6721 gccgctttat ggggttgaag gagcgccgcc gggcgctggc gggacgtacg ggctgtggcg
     6781 tatgcggcgt ggagcaactt aatgacatcg gaaaaccggt gcagccgcta ccgttcaccc
     6841 agacgtttga tctcaacaaa ctggatgatg cattacgtca tctcaacgat tttcagccag
     6901 tgggacaact gaccggttgt actcacgccg ccgcctggat gttgccatct ggcgaactgg
     6961 tcggcgggca tgaagacgtg ggtcgccatg tggcgctgga caaactgtta ggccgccggt
     7021 cacaagaagg ggaaagctgg cagcaaggtg cggtactggt ttccagtcgt gccagttatg
     7081 aaatggtgca aaagtcggcg atgtgcggcg tagagatttt gtttgcggtg tctgccgcga
     7141 ccacgcttgc tgtagaagtg gctgagcgct gtaatctgac actggtaggt ttttgtaaac
     7201 cgggtcgggc aacggtttat acccatccgc agcgtttgag caattaatta aaataagtcc
     7261 tgcgatatat attaaattgc aggacttatt catttcgtga attttattat tttatttata
     7321 aacaaaatgc gtgtagtggc gttttctttt tttcatttat tatgttcact ctcttatcta
     7381 tatatttata aggcaattaa atgaaaagga atttattatc ttccgctatt atagtcgcca
     7441 tcatggccct cggtctgacg ggttgtgatg ataaaaaagc cgaaacagaa acgcccctgc
     7501 ctgccaatag tcaacctgcc gcaccagctc ctgaagcgaa acctactgaa gctcccgttg
     7561 caaaagcaga tgctaaacct gaaacacctg cgcaaccgct ggtcgatgaa caagcggttt
     7621 tcgacgaaaa aatggatgtc tatatcaagt gctacaacaa gttacagatc ccggtacagc
     7681 gcagtctggc gcgttatgct gactggctga aagattttaa acaggggcct accggtaaag
     7741 agagtactgt ttatggcatc tacggcatta gtgaatccaa cctcgctgag tgtgaaaaag
     7801 gcgtaaaaag tgctgtggcg ttaacgcctg cgctgcaacc aattgatggc gtggcggtga
     7861 gttatatcga tgctgccgtg gcgttgggta ataccattaa cgaaatggat aaatattaca
     7921 cccaggaaaa ttataaagac gatgcgtttg cgaaagggaa aacgctgcac cagacattct
     7981 taaaaaatct ggaagccttt gaacctgtag cagaatctta tcatgcggcg attcaggaaa
     8041 ttaatgataa gcgccagctt gcggaactga aaaatattga agaaagagaa gggaaaacat
     8101 tccactacta ttctctggca gtcatgattt cagcgaaaca aattaataac ctgatatcgc
     8161 aagagaagtt tgatgttgat gcggcaatga agaaagtgtc tgaactggaa acgctggtgg
     8221 cgcaggccaa agaagcggat aaaggcggca tgaatttctc gttcattaat tcggcagacc
     8281 agtatcaact cgagactaaa aaatatgttc gccgcgtcag agataaagtc ccgtactctg
     8341 actgggataa agagcatctt caggatgcaa acacaagctg gatggtcgaa gactcatttc
     8401 cacgagcgtt acgcgagtac aacgaaatgg ttgatgacta taatagcctg cgttaaacgt
     8461 ttttgatgat aaatgcagtg tcggatgcgg cgcatccgac acagttcacc ccttgtgaag
     8521 taactccata aatgcctcat atcctgttag cccggcaata acttctgccg gatgctgaca
     8581 caatacgctt gccagatagc taaatatttt tagctcatga cgcgcatcgg cggcggcgca
     8641 ggcgatcaag acatggttaa tgacttcgtt attcacctca actggttggg cgagggtaat
     8701 aaaaaatcca cgaaaacgtc gctcctgttc gctccagcaa tgtgggatgg cgaggcgatt
     8761 aacaatcagg ttttcacctt cgccttcgcg ggcgatgatc cgttgcgctt catcggcggt
     8821 aatatggtgt tgtgcgacta attgcgcaca aatttgccgg gtaatatgtt gccagctttc
     8881 accgcgtaca ttcgaataat gaaaacttcc gggggcagag aaaaaacgct ccggttgctg
     8941 gcgaataaat gccgtcgcca gaaaatgttt tatttgttcg ataccggcag ccgtaatgat
     9001 attttttacg gtgatgtaat tatttaccgc gtcatccagt aaataatggc tgttgttaat
     9061 gatcaataac ggctcaatct cttcgcgaat ggcaacaagt tcgcttaagc tacgggcaat
     9121 aatcacccga caatgtaaaa catcgcgctc aatggcgagc tgattaatag tggcaatcgc
     9181 gttctggtcc gagagcaaaa tgatcggctg gcgttcgttt tgatgtcgtt ccagcgcaca
     9241 ggcaaaatac aaaccgatca gatcgctgtc gaaaagggga atatcgagct gttcacgcag
     9301 tagcgtaata aagtgcagac tcatatcaaa ggccgcaggc caggcggctt tcaggttatt
     9361 catgctgctt tggcgatgct cagcaatcca cactggtgcg gcaaggcaac gtaataaatg
     9421 accggtaatg ttctcaacca gctgcgtatc aataatattc aatgacgcga gacctggcac
     9481 cctttgcagc aaccccagca catgatcggc agaaattacc tgcgcttgct gatgctgttt
     9541 ctctatcaaa cctgtcaggg ttcttacagc gttatcacca agaaacagac cgttatgttc
     9601 aacgatttgt tttatctcgt caccgggata ttgcggccac tcatcggtca gttgattacg
     9661 cagggcgtaa atcgccagaa tcaggctgga gagataatcc ccctgcatga gcggccagcg
     9721 gtgttggttg tcgcaggccg tggataaatg ctgaaggttg tctcgtgtta cgccagccaa
     9781 aggtattaaa aacggatctt tgcgtagcaa gttcgccagc aagataacgc gtttctcttc
     9841 tgtttcatca atgaaatggc ccaaaccggg acgactggcc aggcaacaag tgcgttcata
     9901 acgctgtttt aaccggggca gacgctctgc gacccaagtt tctggcaaat taagcgccga
     9961 ggcgagttgc gcacggggag taaaggtatt cagtaataac agcgccagca gccgatcgtc
    10021 gttatcgtgc ttttgcagca actgaaaaaa gctgcggcgc tcgaagattt ccaactgata
    10081 gcccgcactg ccactggcag agatacgagc tttgccgtta agggtaaagt tgagatagtc
    10141 aatatcacgc aggatggtcc tgcctgaaac gccagtctgt tgcgccagct cgccaggcgt
    10201 gcgcggctgt tgctccagca gatcgacaat ctttaactgg cgttcgttta gcattaatcc
    10261 acctgacgga actggctaaa cgcgttgact ttctccgcag ttaaggtcgt cagaaaatcc
    10321 cgtatcagcg tgagcagagc atcataatcg gcttttgaaa tcataccgct gttagcgtgc
    10381 agataacgag ttggcagaca cagcgcgaca accggacgcc ctccgcccat tacgttgtag
    10441 cgcccgccat ccgtcgctcc ggttttcatg gtggaaaatt gcagcggtaa accgttatgt
    10501 gcggcacagt tttttaacgc cgccaccagt ttctggttgg gtaagtagcg cttgtcaaac
    10561 agcatcagcc ccggcccgtt gcccagtttc agcgggtatt taatgttatc aatgcccgga
    10621 acatcgcccg cgacggcggt atccagcacg atcaccacat ccggtttagt gtgttcagcc
    10681 gaggtttgtg ccccgcgtag cccaacttct tcttccacgc tgccgacgcc gtacagcgta
    10741 attcctgggt tatttactgt ctgtagtagc tcagccatca tcgcgcagcc gatgcgatta
    10801 tccagcgcct tgccgactat tttatcttcg ccccagcagg caaaattggc ttccgggcta
    10861 ataaaatcgc caatttccgc accgcgtttt ttcgcttctt cgcgactgtt cgcgccaata
    10921 tcaatgaaca tctcatcaaa tgacagcggt tgttgctttt gcttttccgt taacgcatga
    10981 ggcgctacgg aaccaatcac accagggatt ttcactccct tgtgtgtgcg tattgttacc
    11041 cggtggttga gcatcgactg attccaccag ccgccaatgg tggtaaagcg cagaaaaccg
    11101 ctctcgtcga tgtgggtgac cataaagccg acttcatcca tatgcccgac aacggcaact
    11161 tttggacctt tatttccctt acgggcaaca aagctgccca gaccatcaaa ggtgatctca
    11221 ttaacgcaag gttccagcgt gtttatcaga atgtcgcgaa cttcctgttc atcgccgctg
    11281 acggcgctgg cttcgcacaa ctgttgcagt aactcaatgt tcatggacaa gctcctgttg
    11341 cgcggctttc gctttgcggc gtaaccacaa tcctttcagg acgataatga atgaaatgtt
    11401 cagcgcgagg ccaatcgcca gcaccagata aaacgaacct accggcgaca tcaggccgat
    11461 cagcgggtca aagataccca aaccaggtgc cagacgtttg attccgaaag cgatcaccag
    11521 cacgccggta atgccgcccg ccagcgtatt ggcggtaatc atcggcaacg gagctgccag
    11581 agcgtaagga atagccggtt cggttgcgac tgtggcaccg accacaatgg cactactggc
    11641 agcctctttt tcctgtcggg taaacagttt gggcgcaatg aacgtcgcaa gacccgccgc
    11701 gacggggaca acgccgacga tggcgtacca gtcataaatg tgtttttcca gcagtgagaa
    11761 gcagaagaac caggcggttt tattgatggg gccgcccata tcgaacgcca gcatcgcacc
    11821 gaccagaaac gctgccgcaa acttcattga tggaggaatg gtattcagga agtgcagtag
    11881 cccgcccatg aggtcggaca tcaccggccc gataacgtag taagtgagca cgccaaaaac
    11941 cagcagagta acgaatggga taagcatgga gccgagcagc ggttgtaatg ctttgccaag
    12001 acggacttta cggaaccaga agacgaaata gccaatcgcc agccccagca ccaccgcgcc
    12061 gaggaatccc gcacccgact gggtgccgag cagcgctttg tcgttcgcca gatagcaaac
    12121 cagaaacgcc ggagcgaaag ccggtttatc ggcaatcgac gaggcaatat aagcgcccat
    12181 gatggggatc ataaaggtga agccaaggta accgatggat tcaactaccc aggtaaacga
    12241 cggcgcgcct ttcgacatat cggtatacgg caaaccgaac tgcaccagca tgttggcaag
    12301 cgccaccagg ataccaccgc cgatgacaaa cggcagcgcc gcagagacgc ccgccatcag
    12361 gtggctcatc acgctgccgc tttgtacttc ctgtttgcct agcttcacgc cgctatctgc
    12421 ggcaaaaagc tgcgaatttg tcggtaattc actgaaaatc tgatcgatat ttttcagcgc
    12481 ctgggagatg gcaatctcat aaactttttt cccggcaaac cgtgcgcgat cgtcaccgct
    12541 caggccacgc ccggtggcga gaatcacgta atcggcagcg gcaatctctt cgctggataa
    12601 gcggttttca acgccgctgg acccttgagt ttccacttta atggtatggc cgagagaacg
    12661 ggctttctgt tccagggctt ccgccaccat gtaggtgtga gcgatcccgg cggggcagtt
    12721 ggtgatcgcg acaatacgta aggatgactc catcgccact tccttatgag ctgagggttt
    12781 gatttaacaa ggcaagcacc tggtcggtat cgccctgttg cagttgatga ataaattcct
    12841 ggtgaataat tttgcgacac agtgtgccga tgattttgac ctgatcctct tcgccacttt
    12901 gcggtacgcc gaggcagatc cagcaattga cgtcttcgcc gtcgctggct ttccagtcaa
    12961 tagcctgcgc tttgcgggcg aataacacga acggttgttt aacgcaggtg cttttaccgt
    13021 gcggcacggc gacaccagaa ccaaaaccgg tggagtgcat tttttcgcgc agcagcaggg
    13081 tttgcaggaa ctggtgtgag tcggtgataa aaccgttttg taacgccatt gtcgccaact
    13141 gcttcagaac ggaataagcg ccattgccct gaatattcag gtcaatacag cttgcagtaa
    13201 gagctgccat taatcggact ccatgaaaaa atatttcagt taattgtaat agcggggcgg
    13261 gtttgtgatg agcgcggata tgtcgtgaag tcgtgacaat ataaagcaga gtgaagg
//
LOCUS       DBFIFM010000127        13191 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.127, whole genome shotgun sequence.
ACCESSION   DBFIFM010000127 DBFIFM010000000
VERSION     DBFIFM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.127"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(3..1361)
                     /gene="plaP"
                     /locus_tag="N7C74_003172"
     CDS             complement(3..1361)
                     /gene="plaP"
                     /locus_tag="N7C74_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HGU2432993.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1351..1413)
                     /gene="yoeI"
                     /locus_tag="N7C74_003173"
     CDS             complement(1351..1413)
                     /gene="yoeI"
                     /locus_tag="N7C74_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HGU2432994.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1628..2556)
                     /gene="yeeY"
                     /locus_tag="N7C74_003174"
                     /pseudo
     CDS             complement(1628..2556)
                     /gene="yeeY"
                     /locus_tag="N7C74_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(2602..3426)
                     /gene="yeeZ"
                     /locus_tag="N7C74_003175"
     CDS             complement(2602..3426)
                     /gene="yeeZ"
                     /locus_tag="N7C74_003175"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU2432995.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            3852..3902
                     /gene="hisL"
                     /locus_tag="N7C74_003176"
     CDS             3852..3902
                     /gene="hisL"
                     /locus_tag="N7C74_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HGU2432996.1"
                     /translation="MTRVQFKQHHHHHHPD"
     misc_feature    3878..4001
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4048..4947
                     /gene="hisG"
                     /locus_tag="N7C74_003177"
     CDS             4048..4947
                     /gene="hisG"
                     /locus_tag="N7C74_003177"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HGU2432997.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDDAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTQRLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            4953..6257
                     /gene="hisD"
                     /locus_tag="N7C74_003178"
     CDS             4953..6257
                     /gene="hisD"
                     /locus_tag="N7C74_003178"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HGU2432998.1"
                     /translation="MSFNTIIDWNSCTAKQQRQLLMRPAISASESITRTVNDILDSVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKVGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6254..7324
                     /gene="hisC"
                     /locus_tag="N7C74_003179"
     CDS             6254..7324
                     /gene="hisC"
                     /locus_tag="N7C74_003179"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HGU2432999.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIIAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7324..8391
                     /gene="hisB"
                     /locus_tag="N7C74_003180"
     CDS             7324..8391
                     /gene="hisB"
                     /locus_tag="N7C74_003180"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HGU2433000.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPQLLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTRRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8391..8981
                     /gene="hisH"
                     /locus_tag="N7C74_003181"
     CDS             8391..8981
                     /gene="hisH"
                     /locus_tag="N7C74_003181"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HGU2433001.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            8981..9718
                     /gene="hisA"
                     /locus_tag="N7C74_003182"
     CDS             8981..9718
                     /gene="hisA"
                     /locus_tag="N7C74_003182"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HGU2433002.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAISCWQNA"
     gene            9700..10476
                     /gene="hisF"
                     /locus_tag="N7C74_003183"
     CDS             9700..10476
                     /gene="hisF"
                     /locus_tag="N7C74_003183"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HGU2433003.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            10470..11081
                     /gene="hisIE"
                     /locus_tag="N7C74_003184"
     CDS             10470..11081
                     /gene="hisIE"
                     /locus_tag="N7C74_003184"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HGU2433004.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11178..12155)
                     /gene="wzzB"
                     /locus_tag="N7C74_003185"
     CDS             complement(11178..12155)
                     /gene="wzzB"
                     /locus_tag="N7C74_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HGU2433005.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIESSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKELRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(12301..>13191)
                     /locus_tag="N7C74_003186"
     CDS             complement(12301..>13191)
                     /locus_tag="N7C74_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HGU2433006.1"
                     /translation="NTSSVESVIKDVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENI
                     IFSPEFLREGKALYDNLHPSRIVIGERSERAERFAALLQEGAIKQNIPTLFTDSTEAE
                     AIKLFANTYLAMRVAYFNELDSYAESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGY
                     CLPKDTKQLLANYQSVPNNLISAIVDANRTRKDFIADAILSRKPQVVGIYRLIMKSGS
                     DNFRASSIQGIMKRIKAKGVEVIIYEPVMKEDSFFNSRLERDLATFKQQADVIISNRM
                     AEELRDVADKVYTRDLFGSD"
BASE COUNT         3323 a         3448 c         3374 g         3046 t
ORIGIN      
        1 gattatgcta cgtcttcgta ctgcggaacc gggttacgga agctcttggt gacgcaagcc
       61 aggtaaatca gaccgatagc agcccagatc agacccagaa ccattgagct ttcttccagg
      121 ttaacccaca gcgcaccaac ggtcagcgca ccacacatcg gcaggaacag atactggaag
      181 tgatctttca gcgtcttatt acgcttctca cggatccaga actgcgagat gaccgacagg
      241 ttaacgaagg tgaacgccac caacgcacca aagttgatca gcgccgtagc cattaccagg
      301 tcgaagttga ttgccagcag ggcaatcgcg ccaaccagga tgatgttcat cgccggagta
      361 cgccatttcg ggtgtacata accgaagaaa cttttcggga acacgccgtc acgacccatt
      421 acgtacatca gacgcgctac gcctgcgtgc gccgccatac cggacgccag tacggtaatg
      481 gtggagaaga tcagcgcacc gacctggaac gctttacccg caacgtacag catgatttca
      541 ggctgtgacg catccggatc tttaaagcga gagatatccg ggaagtacag ctgcaggaag
      601 taagttgcaa agatgaagat catgccgccg atcagcgcgg tcaggaaaat cgcacgcggg
      661 atcacgcgct ctgcatcttt ggtttcttcc gacagattgc tgatgccgtc aaagccggta
      721 aaggagaagc acaggattgt cgccccggta atcatcggga taacatgcgc atcgccagac
      781 cagaatggac gagtgctcgc cagcgtacca gcgccttcac cttcaaatac gccataaaca
      841 accatgccca gaatcaccgc gatcagcact acctgcaaca cgacgattac agtgttgaag
      901 ttcgctacgg atttcagact acgcaggtta aaggcggtca taaaggccac cagcgccacc
      961 acaaacatcc acgatgggat ggaaggcacc agagcttcaa aatagatttt cgccagcaga
     1021 atgttgatca tcggcgcgaa cagatagtcg agcagagaag accaacccac cataaagccg
     1081 acagtcgggc taatggattt ctgggcgtaa gtatatgcag agccagcaga aggatagcgg
     1141 cgaaccagct tcccgtagct cagagccgta aacaggatcg caatcaatgc gaacgcatag
     1201 gctgtcggca catgaccatc cgtgaggcct gaaacgatac caaaagtatc aaacagcgtc
     1261 atcggctgca tataggcaag acccatcatt acaaccggaa ctaacgtaag cgttttacgc
     1321 aattccacgc gagaggtgtt tggagtaacg ttatgcgaca tggtcattct cctttacggt
     1381 gataaccgtc gcgtaagcaa aaaattgccc catttttttg gattcctcag cgacaacaac
     1441 tgtcgatttt tagtaaatat ctatccggta cgaagcccgg cctcttggta tgaatgattg
     1501 gtttgaagca aaaaataacc gacgctgatg aaacgtcggt ttttagtcat tttttgacag
     1561 cggcgcattg tgcctaaatg ggggggaaat gacaagagaa tgagaggctt gtcataataa
     1621 tttttcttta aatggctgat tttccgtcat cagattttgc ataaacaccg gcgatcgcac
     1681 tatttgctaa aatttcatcc cgccaccagc cgctggcaag tcctgcggtc tgttcattcc
     1741 agccaatcca gacgggttca aagagagttt gggcgaccac tttcttctcg attaacgcgc
     1801 cactatcgag aaaacgttgc gccagataac ggggtaaata gccgcagccg aggccgctaa
     1861 tttgcagctc cagcttggtt ttaaaatcga aaacggtaat ggcttcttgt tcgtcgagaa
     1921 gttgtgaggc cgtagaagcg cctgcctgcg cagtgtcccc caccacaatc gcccgatatc
     1981 gtttaatgat acgacggttt aatggttctt cttcctgggc taacggatga tgaggcgcga
     2041 ccgcaaagac ttgttccaga tcgcccaacc gcgagaaacc aaactcactg gaagatggtg
     2101 gctcatgcat cgcgccaaca ataatatccg ctcgcccctg agtcagggca tcccatgaac
     2161 caccgagtac gccattgata aacttcaggc gcgtcacgtt atgatgttga taaaaagctt
     2221 caatgagtgg cgcaagcaga gaaaaaggaa acgtgtcgtc cacgccaatc accagctcgt
     2281 tttcccagcc ttcatgcagt ttgatcgcct gcttttccag ttctcgcacg gtatgcagaa
     2341 cttcccgccc cttctctaat aacatttttc cggtacgggt gaatttagcg cggtgaccgc
     2401 tacgatcaag caattgaata ttaaggtcgc tttccagctt gtgaacggtg taactcagag
     2461 cggacggcgt cttgtagagt ttagccgacg ccgccgcaaa actgccttct ttttctaagg
     2521 catcgagatc atcaacacat ccagcagtgg tttcatacac gcccccttgc agtgcatgcg
     2581 gcgtcctgct ggcgtgatcg tttactccag cggcattacc agcggatcgg gatactggta
     2641 ttcaaatccc agttcattac aaatccgact gccatcaata atcttacctt tgccgctgtc
     2701 cagactattt ctgaattgcg gaggttccag ccccagtaaa cgggccatct gcggatagaa
     2761 aacattacgc gcagggtgag ctggcgcaca tatattatag atgtgtccgc ctttaggtgc
     2821 ctgtaacaac agagtgatag cgccaataac atcttctaaa tggactaaat taacaccatg
     2881 ttcaccatca ggcgcggttt ttccggcaaa gaagcgtccg ggatgacgcc ccggtccgac
     2941 caggcccgca agacgcagaa tatcgaccga agtaccgggt aaattgtgca gccagtcttc
     3001 gagttcttct aatactcgtc cactgttggt caccggatta cgcggggtgg tttctttcac
     3061 cgtgccttgc gcgtcgccat agacagacgt tgaactggta aaaataattc ggggaatacg
     3121 atgggccagc gcgctatcca ctaactcttg taccgcttgt aaatagaact catcgccggg
     3181 gccgctacga cgggccggaa gcgtaatgac cagcgcatcg gcatccatca gggcatccag
     3241 atcgtcagaa tcgcaaacta actcaggctc catgcgaagc agatagctat caatgccact
     3301 cattcgggcc gcttcgacac catcttgtgt ggttttactc ccggtgactt gccagcctcg
     3361 cgctgaaagt gacatcgcca gcggcatgcc taaccaccct aacccgacaa ttgcgacctt
     3421 tttcatccgt tatctcctga tacaccttag atctataagg ctacgctagc gtatcaaaac
     3481 tgacaattca ttctatgaat gaaatcgaaa ctgttagcaa atataccatt gcctgatgcg
     3541 ctccgcttat tagtcctaca aaacatcaat atattgaatt taggcaacat ttgtagacca
     3601 gataaggcat tcacgccgca tccggcagga acaaagccca ctttagttac agaacacaat
     3661 gttcttgagg gttttaatga catattttca cgattacttt tttaacctag tcactcttag
     3721 ccccctcctt tccccgctca ttcattaaac aaatccatcg ccataaaata tataaaaaag
     3781 cccttgcttt ctaacgtgaa agtggtttag gttaaaagac atcagttgaa taaacattca
     3841 cagagacttt tatgacacgc gttcaattta aacagcacca tcatcaccat catcctgact
     3901 agtctttcag gcgatgtgtg ctggaagaca ttcagatctt ccagtggtgc atgaacgcat
     3961 gagaaagccc ccggaagatc accttccggg ggctttttta ttgcgcggtt gataacggtt
     4021 cagacaggtt taaagaggaa taagaaaatg acagacaaca ctcgtttacg catagctatg
     4081 cagaaatccg gccgtttaag tgatgactct cgcgaattgc tggcgcgctg tggcattaaa
     4141 atcaatcttc acacccagcg cctgatcgcg atggcagaaa acatgccgat tgatattctg
     4201 cgcgtgcgtg acgacgacat tcccggtctg gtaatggacg gcgtggtaga tcttgggatt
     4261 atcggcgaaa acgtgctgga agaagagctg cttaaccgcc gcgcccaggg tgaagatcca
     4321 cgctacttta ccctgcgtcg tctggatttc ggcggctgcc gtctttcgct ggcaacgccg
     4381 gttgatgatg cctgggacgg cccgctctcc ttaaacggta aacgtatcgc cacctcttat
     4441 cctcacctgc tcaagcgtta tctcgaccag aaaggcatct cttttaaatc ctgcttactg
     4501 aacggttctg ttgaagtcgc cccgcgtgcc ggactggcgg atgcgatttg cgatctggtt
     4561 tccaccggtg ccacgctgga agcaaacggc ctgcgcgaag tcgaagttat ctatcgctcg
     4621 aaagcctgcc tgatacaacg cgatggcgaa atggaagaat ccaaacagca actgatcgac
     4681 aagctgctga cccgtattca gggtgtgatc caggcgcgcg aatcaaaata catcatgatg
     4741 cacgcaccga cccaacgtct ggatgaagtc atcgccctgc tgccaggtgc cgaacgccca
     4801 accattctgc cgctggcggg tgatcaacag cgcgtagcga tgcacatggt cagtagcgaa
     4861 accctgttct gggaaacgat ggaaaaactg aaagcgctgg gtgccagttc aatcctggtc
     4921 ctgccgattg agaagatgat ggagtgatcg ccatgagctt taacacaatc attgactgga
     4981 atagctgtac tgcaaagcaa caacgccagc tgttaatgcg cccggcgatc tccgcttctg
     5041 aaagcattac ccgcactgtt aacgatattc tcgatagcgt gaaagcacgc ggtgatgacg
     5101 ccctgcggga atatagcgcg aagtttgata aaaccacggt taccgcactg aaggtgtctg
     5161 ctgaggaaat tgccgccgcc agcgaacgcc tgagcgacga gctaaaacag gcgatggcgg
     5221 tggcagtaaa gaatattgaa accttccaca ctgcgcaaaa actgccgccg gtagatgtag
     5281 aaacgcagcc aggcgtgcgt tgccagcaag tcacgcgccc ggtagcttca gttgggttgt
     5341 atattcctgg cggctccgcc ccgctcttct caacggtatt aatgctggca actccggcgc
     5401 gtattgcggg ctgtaaaaaa gtggtgttgt gctcaccgcc gccgattgcc gatgagatcc
     5461 tttatgcggc gcagctgtgc ggtgtgcagg acgtgtttaa cgtcggcggc gcacaggcca
     5521 ttgccgcgct ggcgtttggt acggaatctg tgccgaaagt ggacaaaatc ttcgggccgg
     5581 gtaacgcctt tgtcaccgag gcaaaacgtc aggtgagcca gcgtctggac ggtgcggcga
     5641 tcgatatgcc cgcaggcccg tcggaagtgt tggtcattgc cgacagcggc gcaacgccgg
     5701 atttcgtggc ttctgatttg ctttctcagg ctgaacacgg cccggactca caggtgattt
     5761 tactgacgcc tgacgctgat atggcgcatc aagttgccga agccgtcgaa cgccagttag
     5821 cagaactgcc gcgtgccgaa accgcccgcc aggcactgaa cgccagccgc ctgatcgtga
     5881 ctaaagattt agcgcagtgc gtggagatct ccaaccagta cggcccggag cacctgatca
     5941 ttcagacccg caacgcccgc gaactggtcg atggcatcac cagcgccggt tcggtatttc
     6001 ttggtgactg gtcaccggaa tcggcaggtg attacgcctc cggaaccaac catgttctac
     6061 cgacttacgg ctacaccgcc acctgttcca gcctcggact ggcggatttc cagaagcgga
     6121 tgaccgtgca ggaactgtcg aaagtaggtt tctccgcgct ggcttcgacc attgaaacac
     6181 tggccgccgc cgagcgcctg accgcccaca aaaatgccgt tactttgcgt gttaacgccc
     6241 ttaaggagca agcatgagca ccgtgactat taccgattta gcgcgtgaaa acgtccgcaa
     6301 cctgacgccg tatcagtcgg cgcgtcgtct gggcggtaat ggcgacgtct ggctgaacgc
     6361 caacgaatat cccacagccg tggagtttca gcttactcag caaacgctca accgctaccc
     6421 ggaatgccag ccgaaagcgg tgattgaaaa ttacgcgcaa tatgcaggcg taaaaccgga
     6481 acaggtgctg gtcagccgtg gcgcggacga aggtattgaa ctactgattc gcgctttttg
     6541 cgaaccgggt aaagacgcca tcctctactg cccgccaacg tacggcatgt acagcgtcag
     6601 cgctgaaacc attggcgtcg agtgccgcac agtgccgacg ctggaaaact ggcaactgga
     6661 cttacagggc atttccgaca agctggacgg cgtaaaagtg gtctatgttt gcagccccaa
     6721 caacccaacc gggcaactga tcaatccgca ggattttcgc accctgctgg agttaacgcg
     6781 cggtaaggcg attgtggttg ccgatgaagc ctatatcgag ttttgcccgc aggcatcgct
     6841 ggctggctgg ctggcggaat atccgcacct ggctattttg cgcacactgt cgaaagcttt
     6901 tgctctggcg ggccttcgtt gcggatttac gctggcaaac gaagaagtca tcaacctgct
     6961 gatgaaagtg attgccccct acccgctctc gacgccggtt gccgacattg cggcccaggc
     7021 gttaagcccg cagggaatta tcgccatgcg cgaacgggtg gcgcaaatta ttgcagaacg
     7081 cgaatacctg attgccgcac tgaaagagat cccctgcgtg gagcaggttt ttgactctga
     7141 aaccaactac attctggcgc gctttaaagc ctccagtgca gtgtttaaat ctttgtggga
     7201 tcagggcatt atcttacgtg atcagaataa acaaccctct ttaagcggct gcctgcgaat
     7261 taccgtcgga acccgtgaag aaagccagcg cgtcattgac gccttacgtg cggagcaagt
     7321 ttgatgagtc agaagtatct ttttatcgat cgcgatggaa ccctgattag cgaaccgccg
     7381 agtgattttc aggtggatcg ttttgacaaa ctcgcctttg aaccgggcgt gatcccgcaa
     7441 ctgctgaagc tgcaaaaagc gggctacaaa ctggtgatga tcactaatca ggatggtctg
     7501 ggaacacaaa gtttcccgca ggcggatttc gatggcccgc acaacctgat gatgcagata
     7561 ttcacctcgc aaggcgtaca gtttgatgaa gtgctgattt gtccgcacct gcccgccgat
     7621 gagtgcgact gccgtaaacc gaaagtaaaa ctggtggagc gttatctcgc tgagcaagcg
     7681 atggatcgcg ccaacagtta tgtgattggc gatcgcgcga ccgatattca actcgctgaa
     7741 aacatgggca ttaatggttt acgctacgac cgcgaaaccc tgaactggcc aatgattggc
     7801 gagcaactca ccagacgtga ccgttacgct cacgtagtgc gtaataccaa agagacgcag
     7861 attgacgttc aggtgtggct ggatcgtgaa ggtggcagca agattaatac cggcgttggc
     7921 ttctttgatc acatgctgga tcagatcgcc acccacggcg gtttccgtat ggaaatcaac
     7981 gtcaaaggcg acctctatat cgacgatcac cacaccgtcg aagataccgg cctggcgctg
     8041 ggcgaagcgt taaaaattgc cctcggcgat aaacgcggta tttgccgctt tggttttgtg
     8101 ctgccgatgg acgaatgcct tgcccgctgc gcgctggata tctctggtcg cccgcacctg
     8161 gaatataaag ccgagtttac ctaccaacgc gtgggcgatc tcagcaccga gatgatcgag
     8221 cacttcttcc gttcgctctc ttacaccatg ggcgtgacgc tccacctgaa aaccaaaggt
     8281 aaaaacgatc atcaccgtgt agagagcctg ttcaaagcct ttggtcggac cctgcgccag
     8341 gccatccgcg tggaaggcga taccctgccc tcgtcgaaag gagtgctgta atgaacgtgg
     8401 tgatccttga tactggctgc gccaacctga actcggtgaa gtctgccatt gcgcgtcacg
     8461 gttatgaacc caaagtcagc cgtgacccgg acgtggtgtt gctggccgat aaactatttt
     8521 tacccggcgt tggcactgca caagcagcga tggatcaggt gcgtgagcgc gagctgtttg
     8581 atctcatcaa agcctgtacc caaccggtgc tgggcatctg cttagggatg caactgctgg
     8641 ggcggcgcag tgaagagagc aacggcgtcg atttgctggg catcatcgac gaagacgtgc
     8701 cgaaaatgac cgactttggt ctgccgctgc cgcatatggg ctggaatcgc gtttacccgc
     8761 aggcaggcaa ccgcctgttt caggggattg aagacggcgc gtacttttac tttgttcaca
     8821 gctacgcaat gccagtcaat ccgtggacca tcgcccagtg taattacggc gaaccattca
     8881 ccgcggcggt acaaaaagat aacttctacg gcgtgcagtt ccatccggag cgttctggtg
     8941 ccgctggcgc taagttgctg aaaaacttcc tggagatgtg atgattattc cggcattaga
     9001 tttaatcgac ggcactgtgg tgcgtctcca tcagggcgat tacggcaaac aacgcgatta
     9061 cggtaacgac ccgctgccgc gcttacagga ttacgccgca caaggtgccg aagtgctgca
     9121 tttggtggat ctgaccgggg caaaagatcc ggcaaaacgt caaatcccgc tgattaaaac
     9181 cctggtcgct ggcgttaacg ttccggtgca ggttggcggc ggcgtgcgta gcgaagaaga
     9241 cgtggcggcg ttactggaag ctggggttgc gcgcgtggtg gttggctcca ctgcggtgaa
     9301 atcaccagaa atggtgaaag gctggtttga acgtttcggt gccgatgcct tagtgctggc
     9361 gctggatgtc cgtattgacg agcaaggcaa caagcaggtg gcggtcagcg gctggcaaga
     9421 gaattcgggc gtgtcactgg aacaactggt ggaaacctat ctgcccgtcg gcctgaaaca
     9481 tgtgctgtgt accgatatct cgcgcgacgg cacgctggca ggctctaacg tctctttata
     9541 tgaagaagtg tgcgccagat atccgcaggt ggcatttcag tcatccggcg gcattggcga
     9601 cattaacgat gttgctgccc tgcgtggcac tggcgtgcgc ggcgtaatag ttggtcgtgc
     9661 attactggaa ggtaaattca ccgtgaagga ggccatctca tgctggcaaa acgcataatt
     9721 ccatgtctcg acgttcgtga tggtcaggtg gtaaaaggcg tacagtttcg caaccatgaa
     9781 atcattggcg atatcgtgcc gctggcaaaa cgctacgccg aagaaggtgc agacgaactg
     9841 gtgttctacg atatcaccgc ttccagcgat ggccgtgtgg tagataaaag ttgggtatct
     9901 cgcgtggcgg aagtgatcga cattccgttc tgcgtggcgg gtgggattaa gtctctggaa
     9961 gatgccgcga aaattctttc ctttggcgcg gataaaattt ccatcaactc ccctgcgctg
    10021 gcggacccga cgttaattac tcgcctggcg gatcgatttg gcgtgcagtg tattgtggtg
    10081 ggtattgata cctggtacga cgctgaaacc ggtaaatatc atgtgaatca atataccggc
    10141 gatgaaagcc gcacccgcgt cactcagtgg gaaacgctcg actgggtaca ggaagtgcaa
    10201 aaacgcggtg ccggagaaat cgtcctcaac atgatgaatc aggacggcgt gcgtaacggt
    10261 tacgacctcg aacaactgaa aaaagtgcgt gaagtttgcc acgtcccgct gattgcttcc
    10321 ggtggcgcgg gcaccatgga acacttcctc gaagccttcc gcgatgccga cgttgacggc
    10381 gcgctggcag cttccgtatt ccacaaacaa ataatcaata ttggtgaatt aaaagcgtac
    10441 ctggcaacac agggcgtgga gatcaggata tgttaacaga acaacaacgt cgcgaactgg
    10501 actgggaaaa aaccgacgga ctgatgccgg tgattgtgca acacgcggta tccggcgaag
    10561 tgttaatgct gggctatatg aacccggaag ccttagacaa aaccatcgaa agcggcaaag
    10621 tcaccttctt ctcgcgcact aaacagcgac tgtggaccaa aggcgaaacg tcgggcaatt
    10681 tcctcaacgt agtaaatatt gcgccggact gcgacaacga cacgctactg gtactggcga
    10741 atcctatcgg accaacctgc cacaaaggca ccagcagctg cttcggcgac accgctcacc
    10801 agtggctgtt cctgtatcaa ctggaacaat tgctcgccga acgcaaatct gccgacccgg
    10861 aaacctccta caccgccaaa ctgtatgcca gcggcaccaa acgcattgcg cagaaagtgg
    10921 gcgaagaagg cgtggaaacc gcactggccg cgactgtaca tgaccgcttt gagctgacca
    10981 acgaggcgtc tgacttgatg tatcacctgc tggtattgtt gcaggatcag gatctggatt
    11041 taacaacggt gattgagaac ctgcgtaaac gtcatcagtg agttgcgggg taagcggatg
    11101 cgatattgtt gtcgcatccg gcaaaaaaaa cgggcaaggt gtcaccaccc tgcccttttt
    11161 ctttaaaacc gaaaagatta cttcgcgttg taattacgca gagcgttacg ccccagcaca
    11221 atccccgcgc caaccatgcc gcccagcagc accgccagaa tcaaggtgat tgcctttttc
    11281 ggactatcgc gacgaatagg taacgtcggt ttcattacat agcggtaagc atgaatatca
    11341 agatcatcaa cctttaagtt ttcaatatcc aacaggtttt gacgtgtctg atagtagttt
    11401 ggtgagaaca ccaacggacg ggtcgcctca tgcttaatca tcgactccag cgcttcgctc
    11461 cccaaaagga acaacgtatc ttgcgtcaca tcttcagtct gctgaacctg tggctttgcc
    11521 acctgcgcct gattcgcata ctgcaacgct tcctgaatct gacggatacg cagctctttc
    11581 tgctcctgcg caactacttc ctgcgttctt aaagagtcct gcaagttttt ccgtcccaga
    11641 gcaatgttgt ctttgagatc cttttctagc tcttgattca ctttatcatc aacttgctga
    11701 atgtattggg ccaacttcat ttgtgctccc tctgcagttt gcccaacata agaaacagtc
    11761 aatggtaatt gctggttctt aacagaagat tcgatggtaa gtttttctgg ttcttcctga
    11821 ttatccagcg tttctgctaa tgctgagaag gcagaactga agcgaccaat caacgtctcc
    11881 tgcaaatccg atactttcgg tgcagcctga ccatagataa cattcatggc attgttatag
    11941 ccagcaattt gccccacgtc gggctgagtg ataattgctg ttgacgtcca tttctccttc
    12001 gctactgcca aataaccaat agccagagca atagccacaa tgacggaaat gatgattgtc
    12061 atcttgccac gccacaactg cactagtaaa tcaatcaaat caatctgttc cgggtcatgg
    12121 ttttgcccag aaacattatt attttctact ctcatcatta ccctaactga cggaaatttc
    12181 caagtccgca tagtttagcg ataatccctg tgaatgctat aggataagtg ataaaaataa
    12241 tgaattattg cggaggtaac aaaagacaat cagggcgtaa atagccctga taacaagatg
    12301 ttagtcgctg ccaaagagat cgcgggtgta gaccttatct gccacatccc taagctcttc
    12361 tgccatacgg ttggaaataa tgacgtcggc ttgttgtttg aaggtggcga gatcacgttc
    12421 caggcgagag ttgaagaatg agtcttcttt catcactggc tcgtagatga ttacttcaac
    12481 acctttcgcc ttgatacgct tcataatccc ctgaatagaa gacgcacgga agttatctga
    12541 accgctcttc ataatcagac gataaatacc taccacttgc ggcttgcgtg acaaaatggc
    12601 atcggcaata aaatctttac gcgtgcggtt agcatcgaca attgccgaga tcaggttatt
    12661 cggcacagac tggtagttcg ccagtaactg cttggtatct ttcggcaaac aataaccacc
    12721 ataaccaaac gacggattgt tgtaatggtt gccaatacgt gggtcgagac aaacgccttc
    12781 gattatttga cgggaattca gacctaaact ttctgcgtag ctatccagtt cgttaaagta
    12841 cgccacgcgc atcgcgaggt aggtgtttgc aaaaagttta atcgcttctg cttcagtgga
    12901 gtcggtaaac agggttggga tattttgctt aatcgcgcct tcctgtaata acgcagcaaa
    12961 acgttctgcg cgttccgaac gctcaccgat tacaatacgt gacggatgaa ggttatcgta
    13021 aagagcttta ccttcgcgga gaaattccgg agagaaaata atattttcag tacgatattt
    13081 cttatgcatc gcttcggtaa aaccaacggg aaccgttgat ttgatgacca taaccgcata
    13141 aggatttatc tcaactacgt ctttaattac tgattctaca ctggatgtat t
//
LOCUS       DBFIFM010000128        13145 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.128, whole genome shotgun sequence.
ACCESSION   DBFIFM010000128 DBFIFM010000000
VERSION     DBFIFM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.128"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..1007
                     /locus_tag="N7C74_003187"
     CDS             <1..1007
                     /locus_tag="N7C74_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HGU2433007.1"
                     /translation="QGVKSFAQKNNFAITVFASHRNERNEILSVADYSLTEPEDPQKR
                     LQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLTTGATGVDWLTLADEKVTF
                     AQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVKPVTGIYGMGFWRFDDSAS
                     PMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGPEFSVDILADKGEILAAVG
                     RRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRNDVNGNPVLLETNMRPSGGV
                     GYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAVRSITDVIAYPESLSNLLN
                     "
     gene            1017..2159
                     /locus_tag="N7C74_003188"
     CDS             1017..2159
                     /locus_tag="N7C74_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HGU2433008.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            2152..2925
                     /locus_tag="N7C74_003189"
     CDS             2152..2925
                     /locus_tag="N7C74_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433009.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            2927..4006
                     /locus_tag="N7C74_003190"
     CDS             2927..4006
                     /locus_tag="N7C74_003190"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HGU2433010.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            4006..4962
                     /locus_tag="N7C74_003191"
     CDS             4006..4962
                     /locus_tag="N7C74_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HGU2433011.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            4973..6181
                     /locus_tag="N7C74_003192"
     CDS             4973..6181
                     /locus_tag="N7C74_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HGU2433012.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            6199..6666
                     /gene="terW"
                     /locus_tag="N7C74_003193"
     CDS             6199..6666
                     /gene="terW"
                     /locus_tag="N7C74_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HGU2433013.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            6927..7256
                     /locus_tag="N7C74_003194"
     CDS             6927..7256
                     /locus_tag="N7C74_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU2433014.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            7243..7584
                     /gene="tnpB"
                     /locus_tag="N7C74_003195"
     CDS             7243..7584
                     /gene="tnpB"
                     /locus_tag="N7C74_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU2433015.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVDWQQV
                     KGHDLAKWVYQ"
     gene            7666..8496
                     /locus_tag="N7C74_003196"
                     /pseudo
     CDS             7666..8496
                     /locus_tag="N7C74_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(8527..10140)
                     /locus_tag="N7C74_003197"
     CDS             complement(8527..10140)
                     /locus_tag="N7C74_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HGU2433016.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(10171..10521)
                     /gene="tnpB"
                     /locus_tag="N7C74_003198"
     CDS             complement(10171..10521)
                     /gene="tnpB"
                     /locus_tag="N7C74_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU2433017.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(10518..10943)
                     /locus_tag="N7C74_003199"
     CDS             complement(10518..10943)
                     /locus_tag="N7C74_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU2433018.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNERRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            10997..11748
                     /locus_tag="N7C74_003200"
                     /pseudo
     CDS             10997..11748
                     /locus_tag="N7C74_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            12088..12261
                     /locus_tag="N7C74_003201"
     CDS             12088..12261
                     /locus_tag="N7C74_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433019.1"
                     /translation="MSSPEQYLSSSEPASDTSLAVAPAQQNKSTIVRLRSFVCIADDA
                     PEKRVNMSSPEYY"
BASE COUNT         3123 a         3490 c         3458 g         3074 t
ORIGIN      
        1 ttcagggggt aaaatcattt gcacaaaaaa ataattttgc cattaccgtt tttgcctccc
       61 accgtaacga aagaaatgaa atcctttccg ttgccgatta ttctttgact gaacctgaag
      121 atcctcaaaa acgtcttcag tttatccagg aaaccattca gacctacggc atccaccata
      181 ttcatactgg ccgtaacagc cagtggtttg aagaacaccg ttcagccatt gaaccgaccg
      241 gtgccaccct tactaccggt gcaacgggcg tcgactggtt aactctggct gacgaaaaag
      301 ttacttttgc tcagtttatg gagcaaaagg gtctcccggt cgtaccatcc tggcgggtga
      361 atacgctggc agaattaaag acacacctcg cggccccgcc gttcactgac agcccggtat
      421 gcgtgaagcc ggtgacgggt atctatggca tgggattctg gcgctttgat gacagtgctt
      481 cgcctatggc cgtctttaat catcccgaac atcgtctggt cagtccgcaa cagtatattg
      541 cagcagcatc agctgctgag tcgtttaaac cccttgtttt gatgccgtac ctgccaggcc
      601 cggaattttc cgtcgatatc ctcgcggata agggcgaaat actcgcagcc gtgggacgcc
      661 gtaaggaagg ggctatccag tatctggtaa acgaaggaag cgcctgggaa ctggcgtgtg
      721 actgcgcccg tgttatgaag gccgacgggc tggtgaatgt tcagacgcga aacgatgtga
      781 atggcaaccc ggtgctgctt gaaaccaaca tgcgtccgtc agggggggtg ggttataccc
      841 ttcacagcgg tgtgaacctt cctgggttat ttgctgcctt taagctcggt ctgatgtctg
      901 aagatatggt acgccagagc gctaaaaaca ccttttctcc ggttgcggtg agatccatta
      961 cggatgtaat tgcatacccg gaatcactct ctaaccttct gaattaaggc gtatttatgt
     1021 ctgatatcct cgaagatatg atttatcgcc gtaccctctc atgcggtacg attcaggtaa
     1081 cccgtgacca gggtgaagtt tcgctcgatg acctgtttga catcgctgaa agacgtaacc
     1141 cgaaacgcgc ctttctgttt gtcagcaaag tactcggcag gcacattccc gtgccaccct
     1201 cagtcatgcg gcaggcctac agacagcttg ccagccagtt cccctcgaca ctgacaggac
     1261 ccgtactgtt tatcggtatg gctgaaaccg ccgttgggct tggggccggt gtatttgatg
     1321 aagtgcgcca ccagcatcgt gaatctgtct atctgacctc tacccgtcac ccggttgatg
     1381 gcacgttgct ttgcgagttc aaggaagaac acagccacgc caccgatcat ctgatctatc
     1441 tgccagatga tgaagagaaa agacgtcgtg ttaccaacgc acgaacgctg gttttgattg
     1501 atgacgaggc aaccaccggt aataccttta ttaacctgct ttctgccctg cgtaataccg
     1561 gcaagcttca acatattgaa caggttatcg ccgtaacgct gaccgactgg agtggcaatg
     1621 ccctgtcgga gcgcaccccc ttgcccgtca cttcggtttc gcttgtaagc ggtcagtggg
     1681 gatggacccc attacctgat gcacctgtcc cggacatgcc gaaagtcaac gtaacttcac
     1741 gaggagaatg ggacatccag gggaaacagt catggggccg actggggatg ctcgcacctg
     1801 cggccgatct cggccatgag gtctccgtcc acaaggggga acgtattctg gttctcggga
     1861 ccggggaatt cgtctgggag ccgttcctgc ttgctgaacg gctcgaagct gccggagcac
     1921 aggcatttta tggatcgacc acccgctccc ctgtcgccgt tggttatgcc attgagtccg
     1981 ccatttcctt tacggataac tacgggctgg gcatccccaa ttttgtctat aacgtcgccc
     2041 accagcagtt tgaccgcatt cttgtgtgta ctgagacacc cgcagaaagt attgacacgc
     2101 agcttcttaa agcgctggct gaggttgcgc ccgtcgtgga gattgttacc tatgaataaa
     2161 cccgttgttc tgagcgacct tgacgacacg ctctttcaga cccgtcgtaa aatggtggac
     2221 gagctggccc tggagccatt ccgtaccggt gccgttgatc gcaccctgaa tccacgaagc
     2281 tttatgacgg aagaacagtc catgttggtg gactggctcc tggagcaggc tgaactcata
     2341 cccgttaccg cacggggaac tgaagaaatc agccgcgtac ggatcccttt ccactcctgg
     2401 gcaatcacaa cgcacggggc cgtcattctc acgcctgaag gcaaacccga cgaggagtgg
     2461 aaggcccata tgctcggcca gctggctccc taccaggaaa agctgacatc gatgcagcgt
     2521 ctgatcactg aaatgatgga cgcaaaagga atcaatgcct gggcaaggct gaacttcgaa
     2581 tacggtgaaa cggcggttta tatggtgatg aaacaccgcg acagcacccg ccttgacgag
     2641 ctcaatgcca ttgcagatga gatagaaacg gtgtttccga ccgagggctt ctacatccac
     2701 cgcaacagta ataacgtggc ctggcttccc accccggttg agaaagggct ggcagtcagc
     2761 tggctgcttg aaaaacttcg ggctgaacga ggagttttcc ccgtaattgg tttgggcgac
     2821 agcctgagcg atcatcgttt tatgaaactg tgcagctggt ttggcatccc gcgtcagagt
     2881 cagtttgcag atgccatttc acagcgaatt tttggagaaa attaaaatgc agccacatga
     2941 aacatttacc ggctcatacc agcccggtga cgtggaattt ctgctaaagc cggttgtcat
     3001 tgagatgacg ccggttgaga aaaaagaaga actgattcag tccgggaaga aacattattc
     3061 ggacatgctc agccaggagc cagcgcctac acaatggcat cttgatttgt ttcaccgggc
     3121 gctggatcgg ggcgcagaga ggcttgcaaa agaagtcaca cagctcgcta ttgctctggc
     3181 cgaacgcttc ggtgatgagc ccattgtact ggccagtctt gtcagggctg gcgttccgct
     3241 cggcgttatg ctgcaccagg ccctgcgtga catggggaaa acctcatggc attacggtat
     3301 cagcattatc cgggatcgtg gaattgacgg tgcagccctc gacgtcattg aagagcggca
     3361 tggtaccagc ggtattgtct ttgttgacgg atggacaggt aaaggcgcaa ttaccggaga
     3421 gcttgtacgc gcactgaaag atcgcccggg ctatcctgag cagccgcgac tggttgtcct
     3481 ggccgatccc tgtggctgct cctggcttgc agccagtgat gatgactggc tcattccttt
     3541 tggcatcatg ggggcgccgg tatcaggcct gatctcccgg tcagtatggt cctctgaagg
     3601 attacacggg tgcatggttt gcgagcatct cagtgaattc gaatgcagcc ggatgcttgc
     3661 cgataccgtc gctcatttcc gtaagaagtt aacaccgtca tccctcgctc ccctgagctg
     3721 gaatacggaa tcagcccggg tcttatggca gacaagtcgc gacgttatcg cgttcctggc
     3781 cgatgaattt aaagtggaca gcgtcaatcg tattaaaccc ggtattgctg aagcaacccg
     3841 ggctgtatta cgtcgggtac cggaccatgt atttgtgcgt tctattgacg acccggacgt
     3901 tgccttgctt gtagggcttg ctcgtgaaaa gggaatagtt gttacagaaa tggggggaac
     3961 cctcggccag tatcgggctg taaccattat caagaaggta ctctgatgaa aaatcgtctt
     4021 tctccctgga atctgggagc cacgctatac atgcctgcaa cacgggaaga tattgctgat
     4081 gccgtcctgc acgggaaaat cccgggcttg cgctctctgg tgatttgcct ggaggatgct
     4141 gtcagtgaag cagacatccc cgtcgccctc aaaaatctgg agcacctgct gcacgagctc
     4201 agtaacagca tgagtagcct gggtaaaaat gactggcctc tggtgttcat tcgcccccgg
     4261 catgccgaaa tgggcagatg gttaaaagcg cattatgatc tttccgctgt cgacgggttt
     4321 gtgctgccta aatttaccct cagttcgtta gctgaatggt gggatatcat ggtcgggact
     4381 cacctgtgca tgatgccaac gctggaaaca gaagatgtct ttgacgtggt tcagatgcgc
     4441 gagctggcta cccgtctgga ggaacatccc tgccacgacc gcattattgc gcttcgaatc
     4501 ggcggcaacg atcttatgaa cgttgtgtcg cttcgccgtc cccgggacct gacgctgtat
     4561 gacagcccga tgggctacgt catcaaaatg ctcgtttcgg tcttcggccc gcgtgatttt
     4621 gccctgaccg cgcctgtatg tgagcatatt gacgatcatg ccgttatggc cagagagctg
     4681 gctcttgata tggcacacgg gcttgttggg aaaacggcca ttcacccggg tcagatagag
     4741 gtcattcaaa acgcgctgat ggtcactcag ggtgagcatt ctgacgctct gcggatcctg
     4801 aactctaccc aggccgtgtt taagtcgcag ggagcaatgt gtgaacccgc cacacatcgc
     4861 cgctgggcgg ctggcattct ggacagggct cgtttttatg ggttacagaa cgagcaaagc
     4921 gctgatggaa tcagattact taccgtgacc cagcatcatt aaggaaatca taatggggtt
     4981 caggttcaga aagtcgatca acattattcc tggcgttcgc ctcaacctga gtaacggtgc
     5041 accgagcctg agtgtcgggc cgagaggtgc ttccgtttct tttggtagcc gggggaccta
     5101 tgccaatctg ggcttgcccg gtaccgggct gagttaccgt acccggcttg accgggccgc
     5161 gcgttccaga ggtgaaaacc ggacggcaac cgacccgggg ctcagacagg cgcttgagca
     5221 gaaagccgct gaactcatgt cagcggtaac cgcaatccgt aatattcacg agctgacgcc
     5281 ggatccaaaa acaggcatca gctgggcaga gctggaagca gtatacctgc ataacagaac
     5341 gtcgcctttt caggttccgg caccggtgcg tccagaaaag ccagactacc ttgtattgcc
     5401 ggaaaagcct gccgagagcg aaggcattag ttttttgggt aaatggtttg aatcggaatc
     5461 agctaaagct gagcgccacg ccgaaaatct tcgccggtgg cagcaggagc tgattgatgt
     5521 ggagcgtgag aatacccttc gacagcaccg gtaccagcaa caacggacgg cctgggccga
     5581 acagtatgca aactggaagt ttgaagctga agaacatgaa aaacggctcg ccacggctca
     5641 ggcagatgcc cggcagcagt tccggacaga cgccgcgttt ttcgaatcat acctggcggg
     5701 tgtgctggca gaaactgaat ggccgcgtga aacgcttgtt gcatttgaag taaagccgga
     5761 gctatcagca gtcctgctgg acgttgattt agctgagatt gaagatttcc ctgataagat
     5821 ttacggcgtt aatgcccggg gaacggagct gacggaaaaa gccatgacgc aaaaagccgt
     5881 acgcgaaaac tatgcccacc acgtccatgg ctgcttgttc cgcctggtcg gtatcgtttt
     5941 acatacgcta cctttcgaca acgtgattgt gtcaggcttt acgcaacggg tcagtaagcg
     6001 gaccggctat ctggaggatg agtatatcct gtcctgcaaa tgcactcgca gccagatgtc
     6061 gtcagtaaat tttgcaggca taaaacacat tgatccggtt gaagcgttag gcgatcaacc
     6121 ggttattcga aagatgagca gtaccttcat ttttcagcct attgaaccac taacccttta
     6181 agcaggctaa atcttacgat gcaattaaat accagacagg cccggatttt taagcttgcc
     6241 aacttactgg gcaccggcaa gcccgtttcc gctgccgaca ttataaccag tctggaatgc
     6301 tctgagccga cattaacccg ggccctcaaa gagctacgcg agtcatattc tgcggagatc
     6361 aaatacagca aggcaggtca ttcctatcat ctcgttaatc cgggccagtt ggataaaaag
     6421 accctccgcc ggatgaatga agcgctcgca cagaatgctg aactgaaaac cggtgagtcc
     6481 acagggaagg tcgtactcga taaggataaa aaaacagccg tgtcattatc gctacgcatg
     6541 cggattctaa gaaaaattga ccgtctggct gcgctgagtg actcgacccg aagtgaagcg
     6601 gtagagaagc tggctctgca ctccgttgat gaactgataa aagaatacag cgctaaaaag
     6661 tcctgacgct aaatctcaga ccggggtgaa aactccggtt ttgtcatatt tcacctctgt
     6721 attggcgtga tgcaaaatgg catctgaatc gatcatggtt tggcatccat ttccgatcac
     6781 tgtttggcat cacaatcgat cacgatttgg catgcttccg ctcattgatt ggcatcctgg
     6841 tgtcgttacg gggaagtcat cttcacaggg atcttcagtc agttagcatt accgacatcc
     6901 attcaccaac ttaccggaga taatcaatgt caaaaccccg atggacgctt gatcagaaaa
     6961 aacatcacgt tgccgcatgg cgcgccagcg gccttacccg tgaacagtac tgtgaacttt
     7021 acgacattcc gtttaaatcc ctgcgccagt ggccgcagga tgtcgcaaaa gccgaaaaac
     7081 gagccagagc accagaaatt attcctgttt cagtatctgg ctcttccgga atgactgatg
     7141 gcagaccatt gtcggatgag cctgtcactc tcttcctgcc cggtggtatc cggatgtgct
     7201 gccagccttc tcagttaacc gatgttttcc gggctcttcg ccatgctgac gcctgataac
     7261 gtattcctgg tcgtaaaacc cgtcgatatg cgccggggta tcgacacact gacacagtac
     7321 gtccagaacg aacttaacgc tgcatggcat gacggtgctg cttttgtctt caccaataaa
     7381 gtccgctccc gcataaaagt tctccggtgg gacaaacatg gcgtatggct gtgtacccga
     7441 cgactgcacc ggggaagctt ccgctggccc cggaaaggtg atgccacatg gcatctcaca
     7501 caggatgagt ttcactggct ggtctttggg gttgactggc aacaggtgaa agggcatgac
     7561 ctggcaaaat gggtatacca gtaagaaccc gaagatgctc agagcaacgc aataactcaa
     7621 tgaaatataa tgaatatcca tgcgtgatgt catataatcc accgcatgga tatctcgctt
     7681 ctcagtacca ccagtgaccc tgaacagctt cgcgctctgg caattgccat ggtgcaaaaa
     7741 gtcatggctg agaatgcaga acttcagaac cgtatccgca tcctggaaga gcagatgaag
     7801 ctcgcacgcc agcagcgttt cggtaaaaaa tgcgaatcgc tggccgggat gcagcgttcc
     7861 ctgttcgaag aggatgttga cgcggatatc gcagaaatct cggcgcatct ggacaaactg
     7921 cttcctcaga ccggggacga agaaaaaaca acaacacgtc ctgttcgtaa gcccttgcct
     7981 tcacctcttc cccgtgctga aaaggttatc ccgccagctg aggaacgttg tcctgactgt
     8041 gatgcccccc tgcacttcat tcgcgatgaa gtcagcgaga agctggaata tattccggca
     8101 caggtcgtgg tcaaccgcta tatccgcccg cagtacagct gtccctgctg tgagaaagtg
     8161 ttcagcggaa aaatgccggc acatatcctt ccgaaaagtg ctgttgagcc atcggtcatc
     8221 gcacaggttg tcatcagcaa atataccgac catctgcccc tgtatcgcca gcagcacatt
     8281 ttctcccgaa tgggggtgga gctccccgtc agtacaatgg cggatatggt cggtgttgct
     8341 ggcgctgcac tggccccgct ggcaaaattg ttacgccacg agcttctgac acgggatgtt
     8401 attcatgcag atgaaacgtc cctgaggctc cttgataccc ggaaaggcgg taaatcctgc
     8461 tccggctggc tgtgggctta cgtcagcgga gaaagagtaa gcgtcaacgg agcaccgtat
     8521 tgacgcttat ttattggtga gtactacgtt ccatggcagg agttcatcaa ctcggttgga
     8581 aggccattct ggcagtacgc tcaggatatg gcgcagatac gcttccggat cgataccgtt
     8641 cagacggcag gtgccgatca gcccgtacaa cagtgctcca cgctcgccgc cgtgatcgct
     8701 gccaaagaac ataaagtttt tctttccgag acagactgca cgaagcgctc tttccgcagc
     8761 attattatcc gcctccgcca gaccgtcatc actgtaataa cagagggcat cccactgatt
     8821 cagtacatag ctgcacgctt cgcccagtct ggattttttc gacagcgtac cattcttctc
     8881 caccatccat tcatgcagtg acgtcagtaa cactttgctt cgctgctgcc tgacggcaag
     8941 acgctctgac tccggtaatc cccgtatttc atcctcgatg gcgtacagtt cactgattcg
     9001 cttcagggct tcttctgccg tcgcactttt gctgctgatg tatacatcgt ggatttttcg
     9061 ccgggcatgg gcccagcacg caacttctgt cagtgcacca ccttcacgtt ctgcactgaa
     9121 cagccgatcg taaccgctga acgcatccgc ctgcaggata ccccggaagg gacgaaggtg
     9181 ttgtaccgga tgttttccct gcctgtctgg tgagtaggcg aaccagaccg ccggtggctc
     9241 tgatgagccc gcattccggt catcccggac atacgtccag atgcgtcctg tttttgcctt
     9301 ttttctgccc ggtgccagca cttttactgg tgtgtcgtca gtgtgaacct tgcgggtgtt
     9361 catcacgtaa cggtacaggg tatcattcag cagagtcatt aactgacaac atgcgtcaac
     9421 ccagttggag agtaatgccc ggctcagttc gatgccctgg cgggcaaaga tttcactctg
     9481 acgatacagt ggcaggtgtt cgcagtattt tcccgttaac acgcgggcaa gtaatccgga
     9541 gcccgcgatg ccgcgctcta tcgggcggga cggcgctggc gcttcaacta tacagtcaca
     9601 ttttgtacag gcttttttta cccgaacagt gcggatcact ttcagggcgc tactcaccag
     9661 ttccagctgc tcagcactga cttcccccag ataatccagc tcactgccac actccgggca
     9721 acaactttct tcaggctcca gacggtgtat ttcacgggga agatgtgccg gtaacggacg
     9781 acgatgacgt gactgtcgca actggcgggg aacctgcggg tcatcctcac gcccactgta
     9841 acgatcgctt tcctgttcgc gttgtttcag ttgggcctca gcctgttcaa cctcacgctg
     9901 cagtttttca gaacgggtac cgaacaacat ccggcgcagt ttttctatct gggccctcag
     9961 atgttctatt tcccgctcct cctcttcgat cttttcctcg gcacgtgcca gtgcagagcg
    10021 caggaaggcc tccgtctctt caaccagact cagttgctga tctttctgac ggagggcttc
    10081 agcctgctca gagagcagcc tttccagctc agtgatacga atgaggtatt tccgactcat
    10141 gaccgttttt ataatccggc catgacattt ttacaacatt gtcagtgcat taaggcggga
    10201 tgttttgggc tgacgccagt ccagtttatc gaggagcatt gccagctgcg agcgggtaat
    10261 ggatacctta ccgtcacgca ccgcaggcca gataaactgg ccttcctcca gacgtttggt
    10321 gaacaggcac agaccatcag catcagccca cagaatttta atcgtgtcac cccgtcggcc
    10381 gcgaaagata aacaggtgac cggagaaggg gttctcatcc agcacatgtt gtacctgttc
    10441 tcccagaccg ttgaaggatt tacgcatatc agtaacgccg gcaaccagcc agattcgagt
    10501 gcctgatggg agcgagatca tcgtcccctc ccggtcagtt cacggatcaa caccgtgagc
    10561 agctctggtg aaggattttc cagcgtcatg ttaccgtggc ggaactcaac tttacaggaa
    10621 ctggcactga ctgtgctttg tgaaggagtg gataaaagcg gagtaagagc cgccataggc
    10681 tctttctgct catcaggcgt tatctcaaca ggtaataatt caacgccagc gccagaagag
    10741 gttgttaccg gaagacgccg cgatatacgc ctttcgttct gccagagcct gagccatttg
    10801 aacaggaggt tatcattgat atcgtgttcc cgggcaatac gggcaacaga ggctcctggt
    10861 tgtgaagcca gtttaaccat ttgaagttta aactcatttg aaaatgatct gcggggttct
    10921 gaggataata ttttctgttc cataacaggt gtccactagt tgaaaaggtg ggcacctacg
    10981 ttaccaatac tggcttaatg gctacatacg gcggtcagtt tacgcttacg agaaagaagc
    11041 ggtccgccgg tcgtctgttt tgactcgcag accggacggg ccctcagata tcctgaggcc
    11101 tggctgcagg gctggtgtgg cggtacgctg gtgagtgacg gatacagcgt gtacaaatct
    11161 ctggcggata atgaccccgg tattacatct gcctgttgct ggagccacgc aaggcgcggt
    11221 ttcgcaaatc tgtataaggc cagcagggag ccccgtgcag ccatggcgct gagaaaaatc
    11281 gcgggtcttt atcgcattga gaagttcatc cgtgaacgtc ctgtggaaaa aatacggcaa
    11341 tggcgacaac ggtattcccg gccgatagtg aatgacctgt ttgcctggcc tgaagagcag
    11401 gagccgtgct gtccgcccga cggaccacta aacaaagcga ttaattatat cctgaaccgg
    11461 cgcgatgaac tgagctgctt cctgagtgac ggagccgtgc ccctggacaa caatatctgc
    11521 gagcgtgcta tccgcccggt ggtcatgggg cggaaagcat ggttattcgc cggttcactg
    11581 atggccggga accgcgcagc acagataatg agccttctgg aaacggcaaa acgtaatggt
    11641 ctggagccgc atgcctggct gacagacgtt ctgacgcgtc taccggagtg gccggaagac
    11701 cgactggaag agttgctgcc ccttgaggga tttaccttct ccgggtgagt gatacctgcc
    11761 atcaggtgtt cgtgcagcgg gccataaccc gcagtcggga gttgaacgcc tgacgacagg
    11821 aaatgagcca gagcagcgac cacgcgggct gacagtgagt cagaacctga atcagcccgg
    11881 agccattccc ggggctgtac ttcatcacct gccatcagta cccgtgtcag tacttcgcga
    11941 cctgacgcaa ttacccgact cagttcttca gaacccgcct ccgataccag cctggcggtc
    12001 gcccctgtgc aacaaaataa atcaaccatc gtcagacggt gatcaatcgt atgcgctgcc
    12061 agtggctcgc ctgaaaaatg cgtcaatatg agttcgccgg aacaatacct cagttcttca
    12121 gaacccgcct ccgataccag cctggcggtc gccccggcgc aacagaataa atcaaccatc
    12181 gtcagactgc gatcattcgt atgcatcgct gatgatgcgc ccgaaaaacg cgtcaatatg
    12241 agttcgccgg aatattactg acgacacggt ttgtagttac gttccttcgg tgagtgagta
    12301 cctgtgcgtc agggtaacgc aatcagttca ggtttagcgg ggtgtcaagt cggtttggct
    12361 aaccgaccgc agaattaact gtacctttaa aggtcgcccc cgatatatct gcctgtcaga
    12421 catatcgccg attcggtatc tcactacata aaactcctta aaaaacaatg cgattctgtt
    12481 ggtacgaagc tttcagtaag tcagtccgga gaagcgccag tacgatacag aatgaggttg
    12541 aaaaaaacgt tcacgtctgg tcacttcttc aaatgttaaa aaacatctcg taaagagcca
    12601 gtttatgcgg gttcgttgag tagacgtgcg agcgtcactt tgtcagcttt aacgctgacg
    12661 aaagtgacgc ccgcacagct tcaaactcca catgcacacg caagcgacca aatttcaaat
    12721 ggtcttatgc cgtgcgcaca gtcataagaa gcgcatgcgg aaagtcagaa aatatcttaa
    12781 gacgcagatt ttaaaggcgt ttttatgcca gtctgccggg aatacacagt agtccggttg
    12841 tccggaagca ccacggtaat ccgaaggaaa aatcaacgta cctcaatcac cgtagtgata
    12901 tccgttccca gaggctaaac ggatgtacca ccataaaggc atcctctttc aggctacgag
    12961 gatattgcaa tcacccgaag gcgtttctct cagattgctg aaacattggc tggcagtcac
    13021 ccgaaggcgt ccctctcaga ctgctggaac attggctggt cgcaaaagcg acagttaatg
    13081 caaatacagg ttaacaaaaa gggaaatctg ccgtcaaccc aaccatatga acatgttgat
    13141 ttcat
//
LOCUS       DBFIFM010000129        12815 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.129, whole genome shotgun sequence.
ACCESSION   DBFIFM010000129 DBFIFM010000000
VERSION     DBFIFM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.129"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            95..1609
                     /gene="caiT"
                     /locus_tag="N7C74_003202"
     CDS             95..1609
                     /gene="caiT"
                     /locus_tag="N7C74_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HGU2433020.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFLWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1640..2782
                     /gene="caiA"
                     /locus_tag="N7C74_003203"
     CDS             1640..2782
                     /gene="caiA"
                     /locus_tag="N7C74_003203"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HGU2433021.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2911..4128
                     /gene="caiB"
                     /locus_tag="N7C74_003204"
     CDS             2911..4128
                     /gene="caiB"
                     /locus_tag="N7C74_003204"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HGU2433022.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKARETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4202..5755
                     /gene="caiC"
                     /locus_tag="N7C74_003205"
     CDS             4202..5755
                     /gene="caiC"
                     /locus_tag="N7C74_003205"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HGU2433023.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDTFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYDAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFIDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5864..6649
                     /gene="caiD"
                     /locus_tag="N7C74_003206"
     CDS             5864..6649
                     /gene="caiD"
                     /locus_tag="N7C74_003206"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HGU2433024.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEESYSYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6655..7245
                     /gene="caiE"
                     /locus_tag="N7C74_003207"
     CDS             6655..7245
                     /gene="caiE"
                     /locus_tag="N7C74_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HGU2433025.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYTDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7364..7759)
                     /gene="caiF"
                     /locus_tag="N7C74_003208"
     CDS             complement(7364..7759)
                     /gene="caiF"
                     /locus_tag="N7C74_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HGU2433026.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(8020..11241)
                     /gene="carB"
                     /locus_tag="N7C74_003209"
     CDS             complement(8020..11241)
                     /gene="carB"
                     /locus_tag="N7C74_003209"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HGU2433027.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11259..12407)
                     /gene="carA"
                     /locus_tag="N7C74_003210"
     CDS             complement(11259..12407)
                     /gene="carA"
                     /locus_tag="N7C74_003210"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HGU2433028.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         3046 a         3310 c         3368 g         3091 t
ORIGIN      
        1 tccatgaggt taataataat tatattaaat gttaacaaaa ataaaacaaa cgggaacgca
       61 aaataaatat tcgttttcac agtggaatta actcatgaag aatgaaaaga gaaaaacggg
      121 aatagaaccg aaggttttct ttccgccatt aataatcgtc ggcatacttt gttggcttac
      181 agtcagagat ctggatgcgg cgaatgtcgt tattaatgct gtattcagtt acgtcaccaa
      241 tgtatgggga tgggcatttg aatggtatat ggtggtgatg cttttcggtt ggttctggct
      301 ggtgtttggc ccgtatgcca aaaagcgttt aggtaacgaa ccacctgaat ttagtaccgc
      361 cagttggatc tttatgatgt tcgcctcctg tacgtctgct gccgtactgt tctggggatc
      421 gattgagatc tactactaca tctccacccc gccgtttggc ttagaaccga actcgacagg
      481 agcgaaagag ttggggctgg cttacagctt gttcctctgg ggacctctgc cgtgggccac
      541 ttacagcttc ctttcagtcg ccttcgctta cttcttcttt gtccgcaaaa tggaagtgat
      601 tcgccccagc tccaccctgg tgccgctggt aggtgaaaaa cacgccaaag ggttgttcgg
      661 cactatcgtc gacaacttct atctcgtcgc cttgatcttc gcgatgggta ccagtctggg
      721 ccttgccacg ccgctggtga ccgagtgtat gcaatggttg tttggcattc cgcataccct
      781 gcaactggac gctatcatca ttacctgctg gattatcctc aacgccattt gcgtcgcctg
      841 cggtctgcaa aaaggggtac gtatcgccag tgacgtgcgt agttacctga gcttcctgat
      901 gctgggttgg gtgttcatcg tcagcggtgc cagcttcatc atgaactact tcaccgattc
      961 ggtggggatg ttgctgatgt atctgccgcg catgttgttc tataccgatc ccatcgctaa
     1021 aggcggcttc ccgcagggct ggaccgtgtt ctactgggca tggtgggtga tttacgccat
     1081 ccagatgagt atcttcctcg cccgcatctc ccgtggtcgt actgtgcgtg aactgtgctt
     1141 cggcatggtg atgggactga cagcatccac ctggatcctg tggactgtac tcggtagtaa
     1201 cactctgctg ttgatagata aaaacatcat caacattcca aatctgatcg aacagtacgg
     1261 tgtggcgcgc gccatcatcg aaacctgggc cgctctgccg ctcagcaccg ccaccatgtg
     1321 gggcttcttc atcctctgct ttattgccac cgttacgctg gttaacgcct gctcttatac
     1381 cctggcgatg tccacttgcc gcgaagtacg cgatggtgaa gaaccaccgc tgctggtgcg
     1441 tatcggttgg tcaattctgg ttggcattat cggtattgtt ttgctggcgc tcggcggcct
     1501 gaaaccgatt caaactgcca ttatcgccgg aggatgcccg ctgttcttcg tcaacattat
     1561 ggtgacgctc tcctttatta aagacgcgaa acagaactgg aaagattaat taacccccaa
     1621 aatatcaaga ggttgaaaga tggattttaa tttaaatgat gagcaggaac tgtttgtcgc
     1681 cggtatccgc gaactgatgg ccagcgaaaa ctgggaggcc tattttgccg agtgcgaccg
     1741 tgacagcgtc tacccggaac gttttgtcaa agcactggcg gatatgggta tcgacagtct
     1801 gctgatccct gaagagcacg gtggtctgga cgcggggttt gttactctcg ccgccgtgtg
     1861 gatggagctg ggacgtctgg gggcaccaac ctatgtactg taccagttgc cgggcgggtt
     1921 caataccttc ctgcgcgaag gcacacaaga gcagatcgac aagattatgg ctttccgcgg
     1981 caccggtaag cagatgtgga actcagcgat taccgaaccg ggcgcgggct ccgacgtggg
     2041 tagcctgaaa acgacttata cccgtagaaa tggtaagatt tatcttaatg gtagtaagtg
     2101 ttttattacc agtagcgcct acaccccgta catcgtggtg atggcgcgcg acggggcttc
     2161 tccggacaaa cctgtctaca ccgaatggtt tgttgatatg agcaaaccgg gcatcaaagt
     2221 gaccaaactt gagaagctcg gtctgcgtat ggatagctgc tgtgaaatca cctttgacga
     2281 tgtggaactg gacgagaaag acatgttcgg tcgggaaggt aacggcttta accgcgtcaa
     2341 agaagagttc gaccatgaac gtttcctggt agccctgacc aactacggta cggcgatgtg
     2401 cgcctttgaa gatgcggcgc gctacgccaa ccagcgcgtg cagtttggcg aggctattgg
     2461 tcgtttccag ttgattcagg aaaaattcgc ccacatggcg atcaaattaa actccatgaa
     2521 aaacatgctg tatgaagcag cgtggaaagc agacaacggc accatcacct ctggcgatgc
     2581 agcgatgtgc aaatacttct gcgccaatgc ggcatttgaa gtggtggata gcgcaatgca
     2641 ggtgctgggc ggtgtcggga ttgcgggcaa ccaccgcatc agccgcttct ggcgtgacct
     2701 gcgtgtagac cgcgtttccg gaggatctga cgaaatgcag atcctgacgc tgggtcgtgc
     2761 ggtgctgaag caataccgct aagtccgtgc gctgcctgat gcgacgctga cgcgtcttat
     2821 caggcctacg ggagcacgaa tgtaggccgg ataaggcgtt tacgccgcat ccggcagtca
     2881 tgcgctacat aaatttttca ggagctaata atggatcatc tacccatgcc gaaattcggg
     2941 ccgttggccg gattgcgcgt tgtcttctcc ggtatcgaaa ttgccgggcc gttcgccggg
     3001 caaatgttcg cagaatgggg tgcggaagtt atctggatcg agaacgtcgc ctgggccgac
     3061 accattcgcg ttcaaccgaa ctacccgcaa ctctcccgcc gcaatttgca cgcgctgtcg
     3121 ttaaatattt tcaaagatga aggccgcgaa gcgtttctga aattaatgga aaccaccgat
     3181 atcttcatcg aagccagtaa aggtccggcc tttgcccgtc gtggcattac cgatgaagta
     3241 ctgtggcaac acaacccgaa actggttatc gctcacctgt ccggttttgg tcagtacggc
     3301 accgaggagt acaccaatct tccggcctat aacaccatcg cccaggcctt cagtggttac
     3361 ctgattcaga acggtgatgt tgaccagcca atgcctgcct tcccgtatac cgccgattac
     3421 ttttctggcc tgaccgccac cacggcggcg ctggcagcac tgcataaagc gcgtgaaacc
     3481 ggtaaaggcg aaagtatcga catcgccatg tatgaagtga tgctgcgtat gggccagtac
     3541 ttcatgatgg attacttcaa cggcggcgaa atgtgcccgc gcatgagcaa aggtaaagat
     3601 ccctactatg ccggttgcgg tctgtataaa tgtgccgacg gctacatcgt gatggaactg
     3661 gtgggcatta cccaaattga agagtgcttt aaagatattg gcctcgcaca tctactcggt
     3721 acgccggaaa tcccggaagg cactcagctt atccaccgta tcgaatgccc ttacggccca
     3781 ctggtagaag aaaaactcga tgcctggctg gcggcacata ccatcgcaga agttaaagaa
     3841 cgctttgccg aactgaatat cgcctgtgcc aaagtactga ccgtaccgga actggaaagc
     3901 aatccacagt atgttgcccg tgaatcaatc actcagtggc aaacgatgga tggtcgcacc
     3961 tgcaaagggc cgaacatcat gccgaaattc aaaaataacc ccggacaaat ctggcgcgga
     4021 atgccctcac atggcatgga cacggctgcc attttgaaaa atatcggcta cagcgaaaac
     4081 gacattcagg agttggtcag caaaggtctg gccaaagttg aggactaaac gcgtggcgtc
     4141 agtcggcttg cctgactagc gcccgcccaa taacaaacga acaaaaatgg atagaggtgc
     4201 aatggatatc attggcggac aacatctacg tcaaatgtgg gacgatctgg cggacgttta
     4261 cggtcataaa acggcgctga tttgtgaatc cagcggcgga gtcgttaacc ggtatagtta
     4321 tcttgagtta aatcaggaga ttaaccgcac ggcaaacctg ttttatacgc tggggattcg
     4381 caaaggcgac aaggttgcac tacatctcga caactgcccg gaatttatct tttgctggtt
     4441 cgggctggca aaaattggcg cgattatggt gccgattaac gcccgcctgt tacgcgagga
     4501 aagtgcgtgg atcctgcaaa atagccaggc gtgcctgctg gtgaccagtg cgcaattcta
     4561 tcctatgtat caacagattc agcaagaaga tgccactcaa ttgcggcaca tttgcctgac
     4621 agatgtggca cttcccgctg atgatggcgt gagttcgttt actcaactga aaaatcaaca
     4681 acctgccacc ttgtgctatg caccgccgct atcgactgac gatacggcgg aaattctctt
     4741 cacctccggc accacctccc gaccgaaagg tgtggtgatt acccattaca acctgcgctt
     4801 cgctggatat tactccgcct ggcagtgtgc actgcgtgac gatgacgtct acctgacggt
     4861 aatgcctgcg tttcatatcg attgccagtg tactgcggcg atggcggcgt tttctgccgg
     4921 ggccaccttt gtgctggtcg agaaatacag cgcccgcgcc ttctggggac aggtacagaa
     4981 gtaccgcgcc accattaccg aatgtattcc gatgatgatt cgtacgttga tggtgcagcc
     5041 gccttcagcg aacgatcggc aacaccgcct gcgggaagtg atgttttatc tcaacttgtc
     5101 ggagcaggaa aaagacacat tttgtgaacg cttcggtgtt cgcttgctga cgtcttatgg
     5161 gatgacggaa accattgtgg gcattatcgg cgatcgccct ggcgataaac gacgctggcc
     5221 gtcgattggt cgggcggggt tttgctacga cgcggagatc cgcgacgatc acaatcgccc
     5281 gctcccggca ggtgagatcg gtgaaatctg tattaaaggc gtaccaggga aaaccatctt
     5341 caaagagtat tttctcaacc cgaaagccac tgcaaaagtg ctggaagctg atggctggct
     5401 gcataccggc gataccggat accgcgacga agaaggcttt ttttatttca tcgatcgccg
     5461 ctgcaatatg atcaaacgcg gtggcgagaa tgtctcctgc gtggagctgg aaaatatcat
     5521 cgccacccat ccaaaaattc aggatatcgt ggttgtgggt attaaagatt cgattcgcga
     5581 tgaagccatc aaagcatttg tggtgctgaa tgaaggtgaa acattgagcg aagaggaatt
     5641 tttccgcttc tgcgaacaaa atatggcgaa atttaaagtg ccctcttatc tggagatcag
     5701 aaaagatctg ccacgtaatt gctcggggaa aataattaga aagaatctga aataaatgaa
     5761 acggcaggga actaccctgc cagctaataa tcacgctcta aaacaatatg cattttttgc
     5821 agggatgttg tcgtccctga aaaagcaaaa atggagaaaa ggaatgagtg aatcattaca
     5881 tctgacccgc aatggatcaa ttctggaaat tactcttgat cgcccaaaag cgaatgctat
     5941 tgatgcaaaa accagctttg aaatgggcga agtatttcta aatttccgtg acgatccgca
     6001 attacgtgtc gccattatta ccggtgccgg agagaaattc ttttccgcgg gctgggattt
     6061 aaaagcggca gcagaaggcg aagcaccgga tgctgacttt ggcccgggtg gttttgcggg
     6121 attaaccgaa attttcaatc tcgacaaacc ggttatcgca gctgtgaacg gctacgcctt
     6181 tggcggcggt ttcgaactgg cgctggcggc agattttatt gtttgtgccg ataacgccag
     6241 cttcgccctg ccggaagcca aactgggcat cgttcctgac agcggcggtg tgctgcgtct
     6301 gccgaagatc ctgccgcctg ccatcgtcaa tgaaatggtg atgaccggca gacgaatggg
     6361 cgcagaagag gcgctgcgtt gggggatagt caaccgcgtg gttagccagg cggaactgat
     6421 ggataacgcc cgcgaactgg ctcagcagct ggttaacagc gccccgctgg cgattgcggc
     6481 gctgaaagag atctaccgca ccaccagcga aatgccggta gaagaatcgt atagctatat
     6541 tcgcagcggc gtgttgaaac actatccatc ggttctgcat tcggaagatg ccattgaagg
     6601 gccgctggcg tttgccgaga agcgcgatcc ggtgtggaaa ggacgttaac aaccgtgagc
     6661 tattacgcct ttgaggggtt aattccggtg gttcacccga cggcgtttgt ccatcccagt
     6721 gccgtcttga ttggcgatgt gattgtggga gccggtgtct acatcggccc actcgcctca
     6781 ctgcgtggtg actacgggcg gttgatcgtg caagcgggag ccaatattca ggatggctgc
     6841 attatgcatg gctacaccga cactgacacc atcgttgggg aaaacggcca tatcgggcac
     6901 ggagcgatcc tgcatggttg tgtgattggt cgcgatgcat tggtcgggat gaacagcgtg
     6961 attatggatg gcgcggtcat tggcgaagag agcattgttg ccgccatgag ctttgtcaaa
     7021 gcaggctttc acggcgagaa acgccagttg ttgatgggta cgccagcccg cgctgtacgc
     7081 agtgttagtg acgacgagtt acactggaaa cggttgaata ccaaagagta tcaggatctt
     7141 gtcgggcgct gtcatgcatc gttacatgaa acacagccac tgaggcaaat ggaggaaaat
     7201 cgcccccgtt tgcaggggac gacggatgtg acgcctaaac ggtgatcttg ccggatgcgg
     7261 cgtaaacgcc ttatccaact ctgctggaga gcaaccgtag gccggataag atgcgccagc
     7321 atcgcatccg gcgaccaggt cgcacggtat catcgaaatg acattaacga cgcatactct
     7381 ttgacaacat catctgccac ttctgctcgc ggttaagttc cgtgagcgga acggcaggaa
     7441 tacgcggtgt ctttcttacg ccgactaatt tttcccgact gttattgcgc aggcgcgcgt
     7501 aaatttgctc atcgatatcg accactttaa ccagtcgctg acactggcat ccccgccctt
     7561 ccagcttatt agggatcatc ttaacttcgc agcttatttc tgtgacttcc gacagaatat
     7621 aagtcagggt attaaccgcc ttgctgtgtt caatatcgaa atgaatagag atctctcttg
     7681 ctatcaccca ccgattttca gccatcatcc attcggcgat taacaagtag agtggttttt
     7741 caacatatcc ttcacacata cgaactccag tttcacaaat taaaagtctg gctatgactc
     7801 gttttgaagc aattaaacaa ggccatatta aaattgtgga tatatcgata gtatgcaact
     7861 tcaccgtcga taagtgtgag ctaaatcctg cttatgtcat caaaaatatc gattccccaa
     7921 aagatttact taatgggcaa aaaaaacggt cctcatcaga gaaccgtatt agttattcga
     7981 tcaaattagc ggatgaaaaa tatccgccat gacacgctat tatttgatct gtgcgtgcat
     8041 ttcctgcacc gaaattactt tttcagtcgc atcggcattc agcgccatcg cggtagcgaa
     8101 accaccgttc agggtggtgt cataatgcac tttatattgc agcgcactgc gacggatcac
     8161 gcgggagtct tcaatcgcac gacggcctga ggtggtgttg atgatgtagg tatattcgcc
     8221 attcttgata cggtcctgaa tgtgcggacg gccttcatgc accttgttta ccagacgcgg
     8281 attgataccc gcttcgccca gcacaatcgc cgtgccgtgg gtcgcatcca gctcgaagcc
     8341 ctgtttcagc agttttgccg ccaggtccac cacgcgttct ttatcgcctt cgcgcacgga
     8401 aagcagcgca cgaccgtgtt tcttcatggt ggagttgctg cccagctgcg ctttggcaaa
     8461 cgcttcagcg aaggtgcggc ccacgcccat gacttccccg gtagaacgca tttctggccc
     8521 taacagcggg tcaacgcccg ggaatttatt gaacggcagc accacttctt tcaccgagta
     8581 gtacggcggg ataacttctt tggttacgcc ctgctcagcc agcgatttgc cagccatcac
     8641 acgcgccgcc acttttgcca gcggtacgcc ggtggctttg gagacgaacg gaacggtacg
     8701 cgccgcacgc gggttaactt caatcaggta gacttcgttg tttttcaccg caaactgcac
     8761 gttcatcagg ccgcgcacct gcaattcgaa ggccagtttc tgcacctgct ggcgcattac
     8821 atcctgaatt tcctgactta aggtgtacgc tggcagcgaa catgcggagt caccggagtg
     8881 cacgcccgcc tgctcgatgt gctccatgat gccgccaatc agcaccattt cgccgtcgca
     8941 gatggcatcc acgtcaactt ctactgcgtc atcaaggaaa tgatccagca acactggcgc
     9001 atcgttagac acgctaaccg ccgtctggaa gtagcgacgc aggtcagctt cgtcatagac
     9061 gatttccatc gcccgaccgc cgagaacgta agacggacgc accaccagcg ggtagccaat
     9121 ctcttttgcc ttctcaactg ccatttcaat agcagtaacg gtggcgttcg ccggttgttt
     9181 cagtttcaga cggtcaaccg catgctggaa gcgttcacgg tcttccgcac ggtcgatagc
     9241 atccgggctg gtgccgataa ccggtacgcc agcggcttcc agtgcacgcg ccagtttcag
     9301 cggggtctga ccgccgtact gaacgataac gcctttcggc ttctcaatac gcacgatttc
     9361 cagcacatct tccagagtta ccggctcgaa gtagaggcga tcggaagtgt cgtagtcggt
     9421 agagacggtt tccgggttac agttaaccat aatggtttcg taaccgtctt cacgcagcgc
     9481 cagcgaggcg tgtacgcagc agtagtcgaa ttcgataccc tgaccgatac ggtttggacc
     9541 gccgccaagc accatgattt tttcacggtc ggtagacgga ttcgcttcgc actcttcttc
     9601 ataagtggag tacatgtaag cggtgtcggt ggcaaactct gccgcacagg tatccacgcg
     9661 cttgtagacc gggtgcaggt catattggtc acgcagctta cggatttccg cttcgcgtac
     9721 gcccgccagt tttgccaagc gcgcatcggc aaagcctttg cgtttcagct ggcgcaggaa
     9781 ttcagcgttc aggccagtga tgcccacttc cgccactttc tcttccagac gcaccagttc
     9841 ttcaatctgt accaggaacc agcggtcaat gttagtcagg ttgaagacgc cgtccacaga
     9901 caggcccgcg cggaaagcat cggcgatgta ccagatacgc tctgcgcctg cgtctttcag
     9961 ttcgcgacgg attttggtta acgcttccgg gtcatccagg ctcactttcg ggtcgaatcc
    10021 agtcgcaccg acttccaggc cgcgcagcgc tttttgcagg gattcctgct gcgtgcgacc
    10081 aatcgccatc acttcgccaa ccgatttcat ctgagtggtc agacggtcgt tagcaccggc
    10141 gaatttttcg aagttgaagc gaggaatttt ggtaaccacg taatcgatgg acggctcgaa
    10201 ggaggccgga gtacgtccgc cagtgatgtc gttcatcagt tcatcgaggg tgtaacccac
    10261 cgccagtttc gccgccactt tagcaatcgg gaaaccggtc gctttcgacg ccagcgccga
    10321 agaacgggac acgcgtgggt tcatttcgat aacaatcagg cgaccgtttt tcgggttcac
    10381 cgcaaactgg acattggaac caccggtttc aacgccgatt tcacgcagca ccgccatcga
    10441 ggcgttacgc atgatttgat attctttgtc ggtcagcgtt tgggctggcg cgacagtgat
    10501 ggagtcaccg gtgtggatgc ccatcgcatc aaagttttcg atagagcaga cgatgatgca
    10561 gttgtcgttt ttatcgcgca ccacttccat ctcgtactct ttccagccga tcagcgactc
    10621 atcaatcagc aactctttgg tcggagagag atccagaccg cgggcgcaaa tttcttcaaa
    10681 ctcttcgcgg ttataagcga taccgccgcc gctaccgccc atggtaaagg atgggcgaat
    10741 aatgcacggg aagcccacgt cagcggcaac cgccagcgct tcttccatcg tatgtgcgat
    10801 accggaacgc gcggtttcca gaccaatttt cttcatcgct acgtcgaaac gacggcggtc
    10861 ttctgcttta tcaatcgcat cggcagtggc accaatcatg gtgacgccga actcttccaa
    10921 cacgccctga cgttccagct ccagcgcgca gttcagcgcc gtctgaccgc ccatggttgg
    10981 cagcaccgcg tccgggcgct ctttttcaat aatcttacgt accacttccc agtgaatcgg
    11041 ctcgatgtag gtcgcatcgg ccatttccgg gtcggtcatg atggtcgccg ggttggagtt
    11101 caccagaatg acgcggtaac cctcttcgcg cagggcttta catgcttgcg cgccagagta
    11161 gtcaaactca cacgcctgac cgataacaat cgggcccgca cccagaatca ggatactttt
    11221 tatatctgta cgttttggca tggctctttt actcctgatt acttagcggt tttacggtac
    11281 tgctcaatta actcgataaa gtggtcgaac aacggcgcgg cgtcgtgtgg accagggctg
    11341 gcttcagggt gaccctggaa gctgaacgcc ggtttatcgg tgcgatgaat gccctgtaac
    11401 gtaccgtcga acagggattt atgcgtgaca cgcaggtttg caggtaatgt tgcttcatcc
    11461 accgcaaaac cgtggttctg ggcggtgatc atcaccacgt ttttctccac atctttaacc
    11521 ggatggttgc cgccgtggtg accaaatttc attttgacag tcttcgcacc gctcgccagc
    11581 gccagcagct gatgaccgag gcagatgccg aagaccggaa tatcggtttc gaggaatttc
    11641 tggatggcgg taatggcgta atcgcacggg gccgggtcgc caggaccgtt ggagaggaag
    11701 atgccgtctg gattcatttt cagcacatct tccgcagaag tttgcgccgg aacgatggtc
    11761 aggcgacagc ctctatccac cagcatccgc aggatgttgc gtttggcacc aaaatcataa
    11821 gccacgacgt ggaacggcag ctcgtcttct tttttcgctt ctggcaggcc gccggtcaac
    11881 gtccagctcc cttgtgtcca gctataggct tctgcggtgg tcacttcttt tgccagatcc
    11941 atgccattca gacctgggaa cgcgcgggct ttttctaacg ccagcgccgc atccgggtta
    12001 tcgcctgcga taatgcagcc attctgtgcg cctttctcgc gcagtaaacg cgtcagctta
    12061 cgggtatcga tatcggcaat cgccacgatg ttatggcgct tcaggtaaga agagaggtct
    12121 tcggtattac ggaagttgct ggcaatcagc ggcaggtcgc gaatcaccag accttgtgca
    12181 tgtacctgag aagattcttc atcggcgtca ttagtgccga cattgccaat atggggataa
    12241 gtaagagtaa cgatttgacg agaataggaa ggatcagtga ggatttcttg ataaccggtc
    12301 attgaagtat tgaaaacgac ttccccaacc gccgaacctg ttgcccctat ggcccgaccg
    12361 tgaaactggg ttccgtcttc cagaaccaat agcgctgact taatcaaaac accctccaga
    12421 gaatattcac tcactttatt tgcatattaa ttcacaatga tgacgtaaat caatgcaaat
    12481 ctgcttaccg acgaatttct ggcaaacggc ggcattctgg agatatgggc gctaaaagtc
    12541 aacttaatgg caggattttt tatattattt tgtggctcat gcatgtttta caggcattaa
    12601 gcgacaaaaa gatcatctaa cctgttctgg aaaacgcttg cgcagcaaga agattgcatg
    12661 aaataaaaac gagcaggcaa aagtggacca aatggtcaaa atttacatta agacaacaaa
    12721 aatagaagac aaaacatgta atttttggca gttatagaaa aattaaaagc ataacaccaa
    12781 aaataaaaga gctaaaaatt aaagcccttt ataat
//
LOCUS       DBFIFM010000130        12730 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.130, whole genome shotgun sequence.
ACCESSION   DBFIFM010000130 DBFIFM010000000
VERSION     DBFIFM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.130"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..820)
                     /locus_tag="N7C74_003211"
     CDS             complement(<1..820)
                     /locus_tag="N7C74_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000217053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2433029.1"
                     /translation="MTVKYYAILTNQGAARLANATMLGSKLNLTQMAVGDANGVLPTP
                     DPAQTKLINQKRIAPLNLLSVDPNNQSQIIAEQIIPENEGGFWIREIGLYDDEGVLIA
                     VANCPETYKPQLQEGSGRTQTIRMILVVTNTEAITLKIDPSVVLATRKYVDDKVLELR
                     LYVDDQMRNHIAAQDPHTQYAQKHNPTFTGEPKAPTPAAGNNTTRIATTEFVQTAITA
                     LINGAPDTLDTLKEIAAAINNDPKFSTTINNALSGKQPLDETLTHLSGKDVAGLL"
     gene            complement(817..1428)
                     /locus_tag="N7C74_003212"
     CDS             complement(817..1428)
                     /locus_tag="N7C74_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HGU2433030.1"
                     /translation="MSDSRLLPTGSSPLEVAAARACAEIEKTPVSIRELWNPDTCPAN
                     LLPWLAWSFSVDRWDDKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLIEVRE
                     WWQLNEEPGTFRIVVGVLEQGITEEMYQELERLVADAKPASRHLTGLAISLSTTGNIF
                     AGAGCYHGDALTVYPYTPEAIIVGGDYFPASAIHLIDNLRVNA"
     gene            complement(1421..2329)
                     /locus_tag="N7C74_003213"
     CDS             complement(1421..2329)
                     /locus_tag="N7C74_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HGU2433031.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLI
                     ITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELTAP
                     LADIVLNSTQASFCTEYSVVTGGSDE"
     gene            complement(2334..2681)
                     /locus_tag="N7C74_003214"
     CDS             complement(2334..2681)
                     /locus_tag="N7C74_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HGU2433032.1"
                     /translation="MTARYLGMNRSDGLTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLALMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTERSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(2678..3313)
                     /locus_tag="N7C74_003215"
     CDS             complement(2678..3313)
                     /locus_tag="N7C74_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HGU2433033.1"
                     /translation="MNTLANIQELARALRNMIRTGLVVETDLNAGRCRVQTGGMCTDW
                     LQWLTCRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVMVKASTRITLDTPEVVCT
                     NRLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            complement(3380..3832)
                     /locus_tag="N7C74_003216"
     CDS             complement(3380..3832)
                     /locus_tag="N7C74_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HGU2433034.1"
                     /translation="MNEFKRFEDRLTGLTESLSPSGRRRLSAELAKRLRQSQQRRVMT
                     QKAPDGTPYAPRQQQSVRKKTGRVKRKMFAKLITSRFLHIRASQEQASMEFYGGKSPK
                     IASVHQFGLSEENRKDGKKIDYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            complement(3825..4292)
                     /locus_tag="N7C74_003217"
     CDS             complement(3825..4292)
                     /locus_tag="N7C74_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2433035.1"
                     /translation="MLKPDSLRRALTDAVTVLKTNPDMLRIFVDNGSIASTLAASLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDAGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVVGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            complement(4255..4500)
                     /gene="lysC"
                     /locus_tag="N7C74_003218"
     CDS             complement(4255..4500)
                     /gene="lysC"
                     /locus_tag="N7C74_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979584.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HGU2433036.1"
                     /translation="MLCAGCTSAPPAQTPVIVANACPKVSLCPMPGSDPQTNGDLSAD
                     IRQLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            complement(4400..4825)
                     /gene="lysB"
                     /locus_tag="N7C74_003219"
     CDS             complement(4400..4825)
                     /gene="lysB"
                     /locus_tag="N7C74_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HGU2433037.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLAKHENASLRASLDRANNVASGQQ
                     TTIIMLKNQLHVALTRADKNEMAQVALRQELENAAKREAQREKAITRLLNENEDFRRW
                     YGTDLPDAVRRLHQRPACADASDCRQRLPESEPLPDAGQ"
     gene            complement(4813..5238)
                     /locus_tag="N7C74_003220"
     CDS             complement(4813..5238)
                     /locus_tag="N7C74_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HGU2433038.1"
                     /translation="MKKLSLSLMLHVSLALMLALSLIYPQSVAVSFVAAWAILATVIC
                     VVAGGVGVYATEYVLERYGRELPPESLAVKIVTSLFLQPVPWRRRAAALVVMVATFIS
                     LVAAGWIFTALIYLVASLFFRLIRTACRQRFEGREPCQS"
     gene            complement(5253..5750)
                     /locus_tag="N7C74_003221"
     CDS             complement(5253..5750)
                     /locus_tag="N7C74_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HGU2433039.1"
                     /translation="MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            complement(5750..6031)
                     /locus_tag="N7C74_003222"
     CDS             complement(5750..6031)
                     /locus_tag="N7C74_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGU2433040.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            complement(6035..6238)
                     /locus_tag="N7C74_003223"
     CDS             complement(6035..6238)
                     /locus_tag="N7C74_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HGU2433041.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVETVLAANPGLAELGAMLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(6238..6747)
                     /locus_tag="N7C74_003224"
     CDS             complement(6238..6747)
                     /locus_tag="N7C74_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HGU2433042.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(6847..7590)
                     /locus_tag="N7C74_003225"
     CDS             complement(6847..7590)
                     /locus_tag="N7C74_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HGU2433043.1"
                     /translation="MTSPAQRHMMRVSAAMTAQRDAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDTRLLTETLALTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRAPRKTRSVTPAKRGRPKKKAS"
     gene            complement(7594..8667)
                     /locus_tag="N7C74_003226"
     CDS             complement(7594..8667)
                     /locus_tag="N7C74_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HGU2433044.1"
                     /translation="MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTTDTAGGTERQPKDFSKLASNKYE
                     CDQINFDFYIRYKTLDLWARYQDFQLRVRNAIIKRQSLDLIMAGFNGVRRAETSDRSS
                     NQMLQDVAVGWLQKYRNEAPARVMSKVTDEEGHTTSEVIRVGKGGDYASLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNREQDNSEMLAADVIISQKRIGNLPAV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIVENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTPTKVTAEPGA"
     gene            complement(8726..9580)
                     /locus_tag="N7C74_003227"
     CDS             complement(8726..9580)
                     /locus_tag="N7C74_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HGU2433045.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQEVTDRADETSQAFSRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            9754..11526
                     /locus_tag="N7C74_003228"
     CDS             9754..11526
                     /locus_tag="N7C74_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HGU2433046.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNIANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFEYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNSAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFLLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            11526..12560
                     /locus_tag="N7C74_003229"
     CDS             11526..12560
                     /locus_tag="N7C74_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU2433047.1"
                     /translation="MSKKKGKTPQPAAKKMTASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
BASE COUNT         2716 a         3732 c         3481 g         2801 t
ORIGIN      
        1 cgagaagacc ggcaacatcc tttccactca aatgagtcag cgtctcatcc agtggctgct
       61 tacctgacag cgcattgtta atggtggtgc tgaatttcgg gtcattgtta atggccgcgg
      121 caatttcttt cagtgtgtcc agcgtgtctg gcgcgccgtt aatcagagcg gtaatagcgg
      181 tctgaacaaa ctcagtggtc gcaatccgcg tggtgttatt tcctgcggca ggcgttggcg
      241 cttttggttc tccggtaaat gtcggattat gtttctgcgc atactgggta tgaggatcct
      301 gtgcggcaat gtggtttctc atctggtcat ccacatacag ccttaattcc aggactttat
      361 catccacgta tttacgggtc gccagtacca ccgacgggtc gattttcagc gtgatggctt
      421 cggtattcgt gacaaccaga atcatgcgga tagtctgggt acgaccgctg ccttcctgta
      481 actgcggttt gtacgtttcc gggcagttcg ccaccgcaat gagtacgcct tcatcatcat
      541 aaagcccaat ctcacggatc cagaatccgc cctcgttctc agggatgatt tgctccgcaa
      601 taatctggct ctggttgttc gggtcaacac tcagaagatt cagcggcgcg atgcgtttct
      661 ggttaataag ttttgtctgt gccgggtcag gtgtcggcaa gacaccattt gcatcaccaa
      721 cggccatttg cgtcagattc agcttactgc cgagcatcgt cgcgttagcc agccgtgccg
      781 cgccctgatt agtcagaatg gcgtagtatt tcactgtcat gcgtttactc tcaggttatc
      841 aattaaatga atggccgagg ccgggaaata atcccctccg acaataatgg cctccggggt
      901 gtagggataa accgtcaggg cgtcgccgtg atagcatccc gcaccggcaa aaatgttgcc
      961 ggttgtactt aaactgatag ccagtcccgt cagatggcgg cttgcaggtt ttgcatcagc
     1021 aacgagacgc tccagctcct gatacatttc ctcggtaata ccctgctcaa gcacgccaac
     1081 aacgatgcgg aacgtccccg gctcctcgtt gagctgccac cactccctca cctcaatcag
     1141 atagccgagc ggctccacca cacggcgaat cgcaccaata gtgcccttat ggcagtgaat
     1201 gaaatacgca tcgcggataa cagcgcgttt tgtcgcttcc ggccacttat catcccagcg
     1261 gtcaaccgaa aatgaccacg ccagccacgg cagcagattt gccggacagg tatccgggtt
     1321 ccacagctca cgaatactga ccggcgtttt ttcaatttcc gcacaggctc ttgcggcggc
     1381 gacttcaagc ggtgatgagc cggtcggcag caggcgcgaa tcactcatcc gagcctccgg
     1441 tcacgacgct gtattcggtg cagaaagacg cctgcgtact gttgagcaca atgtcagcca
     1501 gaggtgcagt cagttcgaca cgttgcacgc cttccacatg caaagcggca taaatggcag
     1561 acagacggat gtcgcgccca agccggtgct gtgccgtgat gtacgcttcc agttttttca
     1621 cggcagcagc gcggatgggt tcgctttcgg gacctgggta aaggtaaagc gtggcgttta
     1681 tctgatattc aacaatggcg gcagactgca cggtcacgcg gtcggccacc ggcctgacgt
     1741 cctcgccatt aagggcgtta cgcaccacgg ccagcaggtc ttcggatgcg acgccgttat
     1801 tttcacgtga cagcacggag atagtgacgc aggccggaga cggactggtg acagagatat
     1861 ccgcgacacg cccgtcagca ctgcgaccat gatactgata ggctcccacc gacccggcga
     1921 cgcttaagcc ctcaaacgcc tgctgaatac gcagacgata atcggtgtca gactccatca
     1981 ctgccggtgt cggcgggata gtcgaatcat ctgccggggt gataatcagg cgtgtggtgt
     2041 tgtaattggc accaatcaca tcaaggtcat taccggcggc acaggccagc atcaccgccc
     2101 gtgcggcctc attcacacgc tgacgccaga taagctcacg ataagcattt tcctccagaa
     2161 gtttgacgag aggctcggat tccagcgtca gggtacgggc gaccgcctcc tgctggtctt
     2221 ccgggtaaag ggaaatcagt gtcgccttgc gttcggcaag aatggtttca aagtccagct
     2281 cctcgaccac atccggtgcg ggtagctggt tcaggtcgat aatcggcatg gtttcaactc
     2341 acagggatgg ttaacgaaag tggctggccg gtgtcgttgt gctggccggt taacgtgacc
     2401 gtcattcgcc cgtcaaaact gcgctcagta gtgacggatg acagggtgac gcggggttcc
     2461 catttcagca ccgccatgta acaggcgacc ttaatctgca actcaagcgc cggggtctgc
     2521 ggctggtcaa tcattaatgc cagcaacgag ccgtaatcac gacgcatcac ccgtgagccg
     2581 accggtgtac gcaggatatc gccgatactc tggctgatat gctcaaggtc agtgacagtc
     2641 aggccatcac tgcgattcat tccgagataa cgcgctgtca taaaggactc ccggttgtgc
     2701 cgccgctgtc gccggggtgt ttatgggtat gcagtacctt accgtttgat gagagttcac
     2761 caccggtgtg ttcaatgttg ccgcgcatcg tcccgccctt ctgtacttcc agcgtgccgg
     2821 taatcagcct gttggtgcag accacctccg gtgtgtccag ggtgatgcgg gttgacgctt
     2881 tcaccatgac caccggcacc gtggcagtaa cagaatcaga agccgtcacg ctggccgttt
     2941 taattccgct taccgtcagt gcactggttt caggttcata ctcaatcacc gccccgtcag
     3001 ggaaacggat atgcagggca tccgccgacg cagacggcgc ggggttatcg ccggaataaa
     3061 tccccggcag aacaaacgct gtgtcaagtt caccgcccac ggccagaatc agcacctgct
     3121 cccccacgga aggtgcccac catgtgcgcg aacgcccggc acgacaagtc agccactgaa
     3181 gccagtcggt gcacatgccg ccggtctgca cacggcagcg accggcgtta aggtcggttt
     3241 cgacgacaag gccggtgcga atcatgttgc gcagtgcgcg cgcgagttcc tggatatttg
     3301 cgagagtgtt cataacggga aggatgccgc cgggtcatac cggcggcaat gtgacgatga
     3361 ggtgtcagga atggcacaac taacggtcga ggtgagccag gataatctct tcaatcatct
     3421 gcacatcctc accggtaaag ccgagcagag gacgcgccgg ataatcaatt ttcttaccgt
     3481 ctttccggtt ttcttccgac agaccgaact gatgcacgct ggcgattttc ggtgacttcc
     3541 cgccgtaaaa ctccattgat gcctgttcct ggctggcacg gatatgcaaa aaacgactgg
     3601 taataagttt cgcaaacatt tttcgcttaa cgcgaccggt cttttttctg acgctctgct
     3661 gctggcgtgg cgcgtagggt gtgccgtccg gggctttctg tgtcatcacc cgacgctgct
     3721 gactctgccg cagacgtttc gccagttcgg cgctcagtcg tcgacgccct gacggtgaca
     3781 gcgattcagt cagtccggtc agccggtctt caaaacgctt aaactcattc atcccacttg
     3841 ctcaccagtt cgccattgat ataaagctcc atcgggcggg tgaccggctc cggcggcgga
     3901 ggttccggga tattcttcac atgcagtgcg ccgaccacct cactgaccag cgtgcgctcg
     3961 gtcagcatca ggctgatgct gatatcaaag ctgctgtcat tgttgatgtc tgcataaaac
     4021 gtgaagccct ttttctggcc tgcgtcggtg gtcatgatgt cgggctgatt ttcccgcagc
     4081 cacgccagca ccggcacgat gagcaggtca aaatcaccgg taaagtcggt cacaatgaca
     4141 ttgagcgtgt aacgcttttc gaatgacagc gacgccgcca gtgtggaggc aatactcccg
     4201 ttatccacga atatccgcag catatcgggg ttagttttca gcaccgtgac ggcatcagtc
     4261 agcgccctgc gcaggctgtc gggtttgagc atcgttttcg tcctgacagt gtttaatcat
     4321 ttttacctgg ctggcacagc gtgccagcgc gttctcaagc tgccggatat cagcacttaa
     4381 atcgccgttc gtctgcgggt cactgcccgg catcgggcaa aggctcactt tcgggcaggc
     4441 gttggcgaca atcactggcg tctgcgcagg cggggcgctg gtgcaaccgg cgcacagcat
     4501 caggcaggtc agtgccgtac cagcggcgaa aatcttcgtt ttcattgagt aacctcgtga
     4561 tggctttctc gcgctgtgct tcacgcttcg cggcgttctc cagctcctga cgcagtgcca
     4621 cctgcgccat ctcgtttttg tctgccctgg tgagggcaac atgaagctga tttttcagca
     4681 tgatgatggt cgtctgctgc ccactggcga cattgtttgc cctgtccagc gaggcgcgca
     4741 ggctggcgtt ttcatgcttc gccagaaaca gaccggccac cgccagtgat aacaacacaa
     4801 ccagcacaat catcagcttt gacatggttc ccgcccctca aaacgctgac ggcaggccgt
     4861 acgtatcagc cggaaaaaca gcgacgccac aagataaatc agcgcggtaa aaatccaccc
     4921 ggcggcgacc agcgagataa acgtcgccac catcaccacc agagccgccg cccgtctgcg
     4981 ccacggcacc ggctgcaaaa acagcgacgt gacaatcttc acggccagcg attccggcgg
     5041 cagctcccgc ccgtagcgtt ccagtacata ctctgtggca tacaccccga caccaccggc
     5101 aaccacacag ataaccgtcg ccagaatcgc ccaggcagcg acaaaactga cggccacgct
     5161 ctgcggataa atcagggaca gtgccagcat cagcgccagc gacacgtgca gcatcagtga
     5221 aagggataat ttcttcatgg tgtttactcc gtttaagccg gtacgccgcc agcggtacgc
     5281 cagacggtga ccagtttttc cagtgaatgc tcacgctgac cgtaaccggc acccggcagg
     5341 gacgcccaga tattgcgaca gcgtgaaatg gcacgctcaa tgcgtcccgc ccggatgtca
     5401 tccagtgcac cgcgttcgcg gatcaactga atggcgagcc tgtcctgtga caacggactg
     5461 aaatccggca gggcaagctg tttgcggtaa tgcggccaga acaggtaaag ctgctgatag
     5521 cgaccggagg ccgtggattt ttcaccgcga cggttaaaca ccttcgccgg tcggccatgc
     5581 gcgaacgggt ggtcactgta gtcggtgaaa atttccggct ttccgtccag tccggtgact
     5641 atcacgtcat agccccggtt tttcgtcagc ggatgattcg ccgtcccttc ggacacggcc
     5701 agcatgtcga gaaaggcggc gatattctga tgcgtgttaa ttaccggcat tacggtttcc
     5761 ccctgccctt aaagcggcgc tgaatggcaa tctcaatcac ctgataaccg gcgataccca
     5821 gcatggagcc gataccgcac accgcaggca gtgacaggtc aggaaactgc accagaacaa
     5881 caccggcaac catcgagaca aaaccaccga gcaacatgcg cccgataaac agacgcgggg
     5941 tgatgggttc accaccggca agcaccttgc cgacaacaat cagcactcca atcatgaaaa
     6001 gcgacaggac gcttttttct tctgctgtca tgcgttactc ccacagattg acagtttcag
     6061 ccacgggcgc ggtctgaacg tcgggcagtt cgacggcggt gccgtgcggc agcatcgcac
     6121 ccagttcagc cagtcccgga tttgcggcga gcacggtctc aaccacgccc tcagtgcgcc
     6181 cgtaataccg gacacaaatg gcgtcgagcg tgtcgccctg tagcgcaaag gtcttcatca
     6241 gatttgactc acgatgcagc gcggcttgtc ctggatacgt gccaccgccc agcgcatatc
     6301 ccgccacagt tcatcaatgg tgctgtcaat gctgtcggcc ttcttgtcgc ccttggcact
     6361 ggcatccacg ccgcgataac gctcataaag cgatgcggtc gccatcgcac acacggcgcg
     6421 ctcgtagtaa aaaactttga tgctttcacc gtcgatgtcg tccgccggga cgtccgccag
     6481 acgcgtaaaa ccggcagcaa ttttctgttc gcggtactcg tacagctccg cattcgtttc
     6541 cgccatgcct gacttgatgg cctcacgcag acgggcgggg gcgacggtct gctcaaggcg
     6601 catacgttcc cggacgcgct tcgggtcgat atcgggaaaa aagaacgtgt ttttaatcac
     6661 cggctcgtcg cctgccggtt gcgggatgac caccgtaccc tcaccggaca cgggagcctc
     6721 ctttcgcgga ataatcagcg tcatcatgac tacctctgaa aagtcgggcg gtggacgccg
     6781 gtgcagtgtc aggtgattca ccctcactga ccggcgtgcc gccctggcgc ggggcgcatt
     6841 cggttgttaa ctggctttct ttttcgggcg tccacgtttt gccggtgtca cgctccgggt
     6901 cttacgcggg gcgcgggtgg ccgctttggg ctgcggctcc ggcttcggtt tcagctcccg
     6961 ctccagtcgt tcaatctctt ttttgacgcc tgcctgacag tcgagctgtg tcgcacgttg
     7021 caggtgagcc agcgcaccgg cggcatcacc agcgtcacgc agaaacagac cggtgatttt
     7081 gtgcagcttt gcacgcactt catcaggcat gtctgccgtg gcggtcagtg caagggtctc
     7141 cgtcagcagg cgggtatcca cagactcacc ggcagcgtgg gcgcgcatgg ccgcgagcgc
     7201 cacctcctcg gtgaacatat acggcggggt gcggcggtgt ttacccggca tggtcagacc
     7261 gtacttcagg gcataacggg caatctccag cgcaccggca atatcgccgg tatccagacg
     7321 ccacagcatg accgtcatca gaatgtcatc ctgtgcacct ttgccctgct ccagcacgcc
     7381 gttcacccac ggcaaccaga acggcagcag ttcgcgtttt ttcgcggcct tcagctcttt
     7441 tgaataaatc gctttcagtg tgcgctggtc tgcggccagc ttgaccagca tctgctcata
     7501 gacagttgca tgtcgcagcg gggcggcgtc ccgctgcgcg gtcatcgctg ccgagacccg
     7561 catcatgtgg cgctgtgcgg gactcgtcat cggttacgct cccggctctg cggtcacttt
     7621 agtcggtgtg gagaagtcac cgaccttaat tttttccacc agacaaccgg cggcgtagtc
     7681 ttccaccacg taatcaatgt tcattgactc gtagttctcc acgcggtcga gtttcgggtt
     7741 ttccacaatc acgcggcgat ggctgtcatc catgtagtag atggacaggt tttccagctt
     7801 cgtgatgagc atcgcatccg ccgggaagta cgggacgcgt accgccggca ggttaccgat
     7861 gcgtttctgg ctgatgatga cgtcagcggc cagcatctcg ctgttgtcct gctccctgtt
     7921 gacgatggga aaatacttgt ccgccagcag ctgacgcccc acaatcacca caaggtcagg
     7981 gtcttcctga taccacggct caatcaggtt gttggtcgca tccatcacca gtgcatcgag
     8041 gctggcataa tcaccgccct tacccacgcg gatgacctca gaggtcgtgt gaccttcctc
     8101 gtcagtaacc ttgctcatca cgcgcgccgg ggcttcattg cggtatttct gcagccagcc
     8161 gaccgccaca tcctgcagca tctggttact gctgcggtca gaggtttcgg cacgcctcac
     8221 gccgttaaaa ccggccatga ttaaatcaag ggactggcgt ttgataatgg cgttacggac
     8281 acggagctgg aaatcctgat aacgcgccca caggtcaagc gttttgtagc ggatataaaa
     8341 atcgaagtta atctggtcgc attcgtactt gtttgacgcc agcttcgaga agtccttcgg
     8401 ctgacgctcg gtgccaccgg cggtgtctgt ggtgctggcg atggagccgg tgacaccaat
     8461 accaattttt tcccctttca tttcgctgac cggcacaatg ttgatgcggg tcagaaagtc
     8521 agaggactcc tgcatggtgt tcatcagggt ctgggtgacc gacggttcaa cggtgaattt
     8581 tttcgacaca tcaccggcgt cgatgccgtt cagttcggca acacgggaca ggtaggcatt
     8641 aaatttaaag cgggtttcct ggcgcatagt ttttcctgaa attaagggtt aatcgtgaag
     8701 gttttcccgg actggctgac gccggtcagc agttcgtcat cagggcgtca ccgccaccgc
     8761 cggtggcctt gctgcggcgc tgctgggtca gactttcggt gtggtcgaga ctgtttttca
     8821 ggcggctgaa tgcctggctg gtttcatccg ccctgtcagt cacctcctgc ttaagtgcgg
     8881 aaaaggcggt ttccatctca gcgagtcgct gctcagtggc gctcagcttt tcctgcacat
     8941 gttcagcaac agcggtcacc gcttcatgca cgtcattcag acgggcgtca tcgctggcct
     9001 gtttgcggcc aaaaatggat ttcacctttt cggtcagggc ggtgaacacg gtttcaggca
     9061 ggtcttcaaa ttccagctca acaggcgttg ctactgaaat caggttttca gggcttaatt
     9121 tgaagcggtt cagggggttg tgttttgccg tgcggcagaa ttccaggtat tccgtgccga
     9181 ggcttgccgg gtcatcggtg acggccagac ccaccagata acatttgccg gtgttggcaa
     9241 agttcggctg aatttccatt gaggtgtaga ccttctgcgc ggccttgttc atcgcgataa
     9301 ggtcatcggt cggggtgatt ttcgcaaaca gcgcccattt gcctttcagc gccgaatcgt
     9361 catcaatctt ttcggccttc agttcggcca catcgccata acgtttaaaa ataccgtcag
     9421 gcaggatgcc gcgcagatgt tccaggttaa tgcggcaacc atagacacgc gggtcaaagg
     9481 tttcggccat ttcctgaata tcctgcgcac tgatgacacg cccgtcacag gtgtcaccct
     9541 caacgccgat acgaaagaat tttgagactt tttttgccat tgtcaggagt cctgaatagt
     9601 gattagaaga gtcacatgtc ggcatcagtt tcccgacgat gcgcatcctc cgccatcagt
     9661 cccggatggc ttatcactga cacaacagca ccttagcgaa tcgcggggcg cgactcagta
     9721 gccttgccgt gtattcatca cggcgaggta ttcatgacca tcaccacaga caccactctt
     9781 ttacacgacc cgcgtcgtca ggcggcgctg ctgtactggc agggattttc cgtgccgcag
     9841 attgccgcca tgttgcagat gaaacgcccg acggtgcaga gctggaaaca gcgcgacggc
     9901 tgggacagcg ttgcccccat cagccgtgtc gaaatgagtc tggaagcgcg gctgacccag
     9961 ctcatcatca aaccgcagaa aaccggcggt gacttcaagg aaattgacct gctcggacgc
    10021 cagattgaac gactggcacg ggtaaaccgc tacagccaga ccggcaacga ggcagacctt
    10081 aatccgaaca tcgctaaccg caacaaaggc gggcggcgca aaccgaaaaa gaattttttc
    10141 agtgacgagg ctatcgaaaa gctggagcag attttctttg agcagtcttt cgaatatcag
    10201 ttgcactggt atcgcgccgg gcttgagcac cgcatccgcg atatcctgaa atcccgccag
    10261 attggcgcga cgttttattt ttcccgcgag gcgctgctgc gcgccctgaa aaccggtcat
    10321 aaccagattt ttctgtcggc cagtaaaacg caggcgtatg tgttccgcga atacatcatc
    10381 gcctttgccc ggctggttga cgttgacctg accggtgacc cgattgtcct gggcaataac
    10441 agcgcaaaac tgatttttct cggcaccaac tccaacaccg cgcagagcca taacggcgac
    10501 ctgtacgtcg acgagatttt ctggataccg aattttcagg tactgcgtaa ggtggcatca
    10561 ggtatggcct cacagagtca cctgcgctcg acctatttct ccaccccgtc cacgctggcg
    10621 cacgacgcct acccgttctg gtcgggtgaa ctgttcaacc ggggacgcgc cagcgccgcc
    10681 gaacgcgtgg aaatcgacgt cagtcataac gctcttgccg gtgggcttct ctgtgcggac
    10741 ggtcagtggc ggcagattgt caccattgag gacgccctga aaggtggctg cacgctgttc
    10801 gacattgagc agcttaaacg cgaaaacagc gccgacgatt ttaaaaacct gttcatgtgt
    10861 gaatttgttg acgacaaggc gtcggtgttc ccgttcgagg agctgcaacg ctgcatggtc
    10921 gacacgctgg aagaatggga agactatgcg ccgtttgccg cgaatccgtt cggctcccgc
    10981 ccggtctgga ttggttacga cccgtcacac cgtggcgaca gtgccggatg cgtggtgctg
    11041 gcaccgccgg tggtggccgg tggcaaattc agaatacttg agcgtcacca gtggaaaggc
    11101 atggactttg ccacccaggc tgaatccatc cgcaaactca ccgaaaaata caacgtcgaa
    11161 tacatcggaa ttgatgccac cggcctcggt gtcggcgtgt tcctgctcgt tcgctcgttc
    11221 tatcccgccg cacgcgatat ccgctacacg ccggaaatga aaaccgcaat ggtgctcaag
    11281 gcaaaagacg ttattcgccg tggctgtctg gaatatgacg tcagcgccac cgacatcacc
    11341 agctcgttta tggctatccg caagaccatg accagcagcg gacgcagcgc cacctatgag
    11401 gccagccgca gcgaggaagc cagccacgcc gacctcgcct gggcgaccat gcacgccctg
    11461 ttaaatgagc cactcaccgc cggtatcagc accccgctga catccaccat tctggagttt
    11521 tactgatgag caagaaaaaa gggaaaacac cgcaacctgc ggcaaaaaaa atgaccgcca
    11581 gcgccccgaa aatggaggca ttcacctttg gtgaaccggt gccggtactc gaccgccgtg
    11641 acattctgga ttacgtcgag tgcatcagta acggcagatg gtatgagcca ccggtcagct
    11701 ttaccggtct ggcaaaaagc ctgcgtgctg ccgtacatca cagctcaccg atttacgtca
    11761 aacgtaatat tctggcttca acgtttattc cgcacccgtg gctttcccag caggatttca
    11821 gccgctttgt gctggatttt ctggtgttcg gtaatgcgtt tctggaaaag cgttacagca
    11881 ccaccggtaa ggtcatcaga ctggaaacct caccggcaaa atatacccgc cgtggcgtgg
    11941 aggaggatgt ttactggtgg gtgccgtcct tcaacgagcc gacagccttc gcgcccggct
    12001 ccgtgtttca cctgctggag ccggatatta atcaggagct gtacggcctg ccggaatatc
    12061 tcagcgccct taattctgcc tggctgaatg agtcggccac gctgttccgc cgcaagtatt
    12121 acgaaaacgg cgctcatgcc ggatatatca tgtacgtcac cgatgccgtg caggatcgca
    12181 acgatatcga aatgcttcgc gaaaacatgg tcaagtcgaa aggccgcaac aactttaaaa
    12241 atctgtttct ctatgccccg caggggaaag ccgacggcat taaaattatc ccgctcagtg
    12301 aagtggcaac gaaggacgat ttttttaata tcaaaaaagc cagcgccgct gacctgctgg
    12361 acgcgcaccg catccccttt cagttgatgg gcggcaagcc ggagaacgtc gggtcgctgg
    12421 gtgatattga gaaagtggca aaggtctttg tccgcaatga gcttatcccg ctacaggaca
    12481 ggatccgcga gataaacggc tggctcggtc aggaggtcat ccgctttaaa aactactcac
    12541 tggacactga caacggctga acatcgccgc ctgcgggcgg cttttttaca ccccgtcatc
    12601 acgccctcac acgctcacca ccgcacaaaa caccccgcag acacaccaac gcctcaacgg
    12661 gcagactaag cgccatcacg acgcgctcag acgctgaaaa aataaaatca gcaccaccgc
    12721 cagcgcgcag
//
LOCUS       DBFIFM010000131        12423 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.131, whole genome shotgun sequence.
ACCESSION   DBFIFM010000131 DBFIFM010000000
VERSION     DBFIFM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.131"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            137..2278
                     /locus_tag="N7C74_003230"
     CDS             137..2278
                     /locus_tag="N7C74_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HGU2433048.1"
                     /translation="MAKFTPSFSGIKGRALFSLLFAAPMIHATDTATTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHSTRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KNAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESIGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(2342..4234)
                     /gene="parE"
                     /locus_tag="N7C74_003231"
     CDS             complement(2342..4234)
                     /gene="parE"
                     /locus_tag="N7C74_003231"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HGU2433049.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGNCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(4263..4844)
                     /gene="yqiA"
                     /locus_tag="N7C74_003232"
     CDS             complement(4263..4844)
                     /gene="yqiA"
                     /locus_tag="N7C74_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HGU2433050.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(4844..5671)
                     /gene="cpdA"
                     /locus_tag="N7C74_003233"
     CDS             complement(4844..5671)
                     /gene="cpdA"
                     /locus_tag="N7C74_003233"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HGU2433051.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(5696..6118)
                     /gene="yqiB"
                     /locus_tag="N7C74_003234"
     CDS             complement(5696..6118)
                     /gene="yqiB"
                     /locus_tag="N7C74_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HGU2433052.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(6119..6748)
                     /gene="nudF"
                     /locus_tag="N7C74_003235"
     CDS             complement(6119..6748)
                     /gene="nudF"
                     /locus_tag="N7C74_003235"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HGU2433053.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6953..8434
                     /gene="tolC"
                     /locus_tag="N7C74_003236"
     CDS             6953..8434
                     /gene="tolC"
                     /locus_tag="N7C74_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HGU2433054.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTASS
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            8582..9253
                     /gene="ygiB"
                     /locus_tag="N7C74_003237"
     CDS             8582..9253
                     /gene="ygiB"
                     /locus_tag="N7C74_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HGU2433055.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            9259..10419
                     /gene="ygiC"
                     /locus_tag="N7C74_003238"
     CDS             9259..10419
                     /gene="ygiC"
                     /locus_tag="N7C74_003238"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HGU2433056.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(10457..11245)
                     /gene="ygiD"
                     /locus_tag="N7C74_003239"
     CDS             complement(10457..11245)
                     /gene="ygiD"
                     /locus_tag="N7C74_003239"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HGU2433057.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            11388..12161
                     /gene="zupT"
                     /locus_tag="N7C74_003240"
     CDS             11388..12161
                     /gene="zupT"
                     /locus_tag="N7C74_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HGU2433058.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(12219..12389)
                     /gene="yqiD"
                     /locus_tag="N7C74_003241"
     CDS             complement(12219..12389)
                     /gene="yqiD"
                     /locus_tag="N7C74_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HGU2433059.1"
                     /translation="MFIAWYWIVLIVLVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
BASE COUNT         3109 a         3307 c         3066 g         2941 t
ORIGIN      
        1 ctactacatt accgcatgat tgaatgattc tcaattacat atccgatggt aaaataacaa
       61 acggatagtg cttagtgagc aacaagaaac aattttcact cacgacaatc cgtgagtttt
      121 tgggataaca gactaaatgg ctaagttcac accttcattc tcaggaatca aaggtcgggc
      181 gctcttttca ctgctctttg cagcaccaat gattcatgca accgacactg caacgaccaa
      241 agatggcgaa acaatcactg ttacagcgga tgcaaatacc gcaactgagg cgaccgatgg
      301 ttatcaacct ctgagcactt ccacggcgac attaaccgat atgccgatgc tggatatccc
      361 gcaggtggtc aatacggtta gcgatcaggt tctggaaaat cagaatgcaa cgacgctgga
      421 tgaagcgctt tataacgtca gtaacgtggt acagaccaat acattaggcg gaactcagga
      481 cgcctttgta cgtcgtggtt ttggtgctaa ccgggatggc tccatcatga ccaacggcct
      541 gcgaactgta cttcctcgca gtttcaacgc cgccacagaa cgtgtggaag tgctaaaagg
      601 tccggcctcc acgctgtatg gcattctcga tcctggtgga ttgattaacg tcgtgaccaa
      661 gcgcccggaa aaaacattcc atggttctgt ctcagccacg tcctccagtt ttggcggagg
      721 cactgggcaa cttgatatca caggtcccat tgaaggcact cagctggcat accgcctgac
      781 gggggaagtg caggatgaag attactggcg aaacttcggt aaagagcgca gtacatttat
      841 tgccccgtca ctcacctggt ttggtgataa tgcaacagta accatgctct attcccatcg
      901 ggactataaa actccgttcg atcgtggaac gattttcgac cttacgacga aacagcccgt
      961 aaacgttgat cgaaaaatac gttttgacga accgtttaat attacagatg gtcagtccga
     1021 tctggcgcaa ctcaacgcag aatatcatct caatagccag tggacagcgc gctttgatta
     1081 cagctacagc caggataaat acagcgacaa tcaggctcgc gttaccgcgt atgatgcaac
     1141 gacaggaacg ctgacacggc gtgttgatgc aactcaggga tctacccagc gtatgcattc
     1201 tactcgtgcg gatctgcaag ggaatgttga tattgctggg ttctataatg agattctggg
     1261 tggggtgtca tatgaatatt atgatcttct gcgcacagat atgattcgct gtaaaaacgc
     1321 taaagatttc aatatctaca accccgttta tggcaatacc agcaaatgca caacggtttc
     1381 ggcgtcggac agtgatcaga cgatcaaaca ggagagctac tcagcttatg cacaggatgc
     1441 gctctatctg accgataact ggattgccgt cgccgggatc cgctatcagt attacacgca
     1501 gtatgcgggt aaaggccgtc cttttaatgt caatactgac agccgcgatg aacaatggac
     1561 gcccaaactg gggttagtct acaaactgac gccatcggta tccttatttg ctaattattc
     1621 gcaaacattt atgccgcaat cgtcaattgc cagctacatc ggagatcttc caccggaatc
     1681 atctaatgct tacgaagtcg gggcaaaatt cgagctgttc gatggtataa ccgcagatat
     1741 tgcgctgttt gatatccata aacgtaacgt gttgtatacc gaaagtattg gtgatgaaac
     1801 catcgccaaa acggcaggcc gcgttcgttc aagaggggta gaagtcgacc ttgcgggagc
     1861 attaactgaa aacattaata tcattgccag ctacggctat accgatgcta aggttctgga
     1921 agatcctgat tatgcaggga aaccattgcc gaatgttcct cgtcataccg gttcgctatt
     1981 cctgacctat gacattcata acatgccagg caataacaca ctgacgtttg gcggtggtgg
     2041 acatggcgta agccgtcgtt cggcaaccaa tggggctgac tattatctgc caggctattt
     2101 cgttgccgat gccttcgccg catacaaaat gaaattgcag tatccggtca cactgcaatt
     2161 aaacgtcaaa aacctgtttg ataaaacgta ttacacctct tccatcgcca caaataatct
     2221 ggggaatcag attggcgatc cgcgtgaagt gcaattcacg gtgaaaatgg aattttgatc
     2281 aaaaaagccc ggtatacact tgtataccgg gctatgtttt attcttctgg cgggaggatt
     2341 atcagacctc aatctccgcc atgtcgcctt tctcttgcaa ccagttgcgg cgatcttccg
     2401 agcgtttctt cgccagcagc atatccatca tcgcgtcagt acgctgatcg tcttcatcat
     2461 cgatagtcag ctgcaccaga cggcgagtgt tcggatcaag cgtggtttcg cgcaattgca
     2521 tcgggttcat ttcccccaga cctttaaaac gctggacgtt cggcttgcct ttcttgcgtt
     2581 ttaattgctc aagtacgccc tctttctctt cttccgtcag cgcgtaataa acctctttcc
     2641 cgagatcaat acggtagagc ggtggcagtg cgacgtaaac gtgaccgtgt ttcaccaacg
     2701 cgcggaagtg ttttacgaac aaagcgcaga gcagtgtagc aatgtgcaaa ccatcagagt
     2761 ccgcatccgc gaggatacag attttgccat aacgaagctg gctcagatcg tcgctgtcag
     2821 gatcgatacc gatcgctacc gaaatatcat gcacttcctg cgaggccagc acttcgtcgg
     2881 aagaaacttc ccaggtgtta aggatcttac ctttcagcgg catgatcgcc tgatattcgc
     2941 gatcgcgcgc ctgcttggca gatccgcctg cggagtcacc ttccacgagg aacagttcgg
     3001 tacggttaag gtcctgcgcg gtacaatcag ccagtttgcc aggcagcgcc gggccgctgg
     3061 tcagcttttt acgcaccact tttttagccg cacgcatacg acgctgggcg ctggaaatcg
     3121 ccatctctgc cagcaactcc gccgcctgaa cgttctggtt cagccacagg atgaaggcat
     3181 ctttcaccac gcccgaaacg aatgccgcac actgacgcga agagagacgc tctttggtct
     3241 gcccggcaaa ctgtggatcc tgcattttta ctgacagcac ataggcgcag cgatcccaga
     3301 tatcttccgc cgacaacttt acaccgcgcg gcaaaatgtt gcggtattca cagaactcac
     3361 gcatcgcgtc cagcaggccc tgacgcaggc cattaacatg ggtaccgccc tgcatcgttg
     3421 ggatcaggtt gacgtagctt tcggtcagca gttcaccgcc ttccggcagc cacagtagcg
     3481 cccagtccac cgcttcagta tcgccagcga aattaccgat aaacggtttt tctggcagcg
     3541 tcggtaaacc gtttaccgct tccgccaggt aatcattcag accgtcctga tagcaccagc
     3601 gctgttcggt gttgttgatc tcatctttaa aggtaatctc aacgccgggg cacagtactg
     3661 ctttggcttt cagcacatgc gtcaggcgtg aaacagaaaa acgcgggcta tcgaagaagg
     3721 tttcatccgg ccagaaatgg acgctggtgc cggtattgcg tttaccgcaa ttgccgacaa
     3781 cctgtaaatc ctgcaccttt tcgccatttt caaaggcgat gttataaacc tgaccatcgc
     3841 ggcgcacgtt aacttctacg cgcttcgaca gggcgttaac caccgaaatc cccacgccat
     3901 gcaggccgcc agagaactgg taatttttgt tagagaattt accgcctgca tgcagacggc
     3961 aaagaatcag ttcaaccgcc ggtacaccct cttccgggtg aatatccacc ggcatcccgc
     4021 gcccatcgtc aataacttct aacgactggt cagcatgtaa aataacgtcc acgcgttttg
     4081 cgtgacccgc cagtgcttca tccacactgt tatcaatgac ttcttgcccc aaatggttag
     4141 ggcgagtggt atcggtatac atccccggac ggcggcgaac cggctcaagc ccggtgagta
     4201 cctcaatggc atcagcgtta taagtttgcg tcatggttta agttagcaat tcgagttgat
     4261 cgtcagagat ggtgcagacc aagaaaatcg acgatcgggt tgaaataatc ttcgaagccc
     4321 gtgaatgcgt ggttgccgcc ttctatgaca gtctggcggc aggaagcgta gtacgccacc
     4381 gcctggcggt aatccagcac ttcatctccc gtctgttgca gcagccagat caaatccggc
     4441 gcttccagcg ggtcaatctg catgacttta agatcgtaaa tatggcgtga ctctagcaca
     4501 tattgctgcc cggtgtaggg gttctcgttc tgaccgagat agtccgtcag cagttcaaac
     4561 gggcgcaccg ccgggtttac caccactgcg ggcagcataa aacattgtga caaccaggtg
     4621 gcgtaatatc cccccagtga cgaaccgaca atacccagcg aatcgccgcc atgttccagg
     4681 acaatggatt ccagcagctc tgccgcgtcg gaaggatacg gcggcaactg cggaatgatc
     4741 atctcaacgt cagggtgatg ttccgccagc cagtttttta acaagctcgc ttttgcagag
     4801 cgcggcgagc tgttgaaacc gtgtaaataa agaagcgtag acatcagtag ccttctgaag
     4861 cggtatcagg ttggaaacgt gtgtccgcca ggcgatgcac ctcggtggtc agcgtgccat
     4921 cagcatgtaa ctcgagagta cgccagccgg gcgcgatagt atccagcgta aagttggaac
     4981 agtgcggctt aaactgcaca caggtcgacg gtgttgccag caggcggcga ccgttccagt
     5041 cgagatccag ctcctgatga atatgaccgc acagcaagta tttgacgtgc ggaaacttcg
     5101 ccagcacggt atccagttcg cccgcgttac gcaggctgtg ttgatcaagc caactacaac
     5161 ccgcaggtag cggatgatga tgcagcagca gcaacgtatg gcgctctggc gcatcagcca
     5221 gtttacgttc cagccactca agctgaaact cgcttagctc accgtgcggc acgccaaaca
     5281 cctggctatc cagcaacagg atttgccatt gctcaccaat aaacacccgc ttcgccgggg
     5341 agatacccgc atcctgtaac gcgctgtaca tcgcgggctg gaaatcgtgg ttgcccggca
     5401 gccagacgca gggcgcacga aaacttgcga tgccttcagc gaaatgctga taggccgcag
     5461 aggattgatc ctgcgctaaa tcacctgtcg cgacaatcag gtcgaattcg tgctggtgtg
     5521 gacgaatcgc ctccagcacc gcctggtagc tttcccaggt gtttacccct aagagggctt
     5581 cgtgcttttg tgcaaacagg tgagtatcgg taatttgtaa aatcctgact ctggcctcac
     5641 cagccagagg aagggttaac aggctttcca aatggtgtcc ttaggtttca cgacgctaat
     5701 aaaccggaat cgccatcgct ccatgtgcta aacagtatcg caaccagtcc gctaaaaact
     5761 ggttaatttg atgcttttcg tcgcgttgat gcaacttttt attaggataa tcataccgcg
     5821 ctttgaagcg aaaaatctgc tggcttgaac acacttcagc caccatcgcg tcatgataca
     5881 gacgcaccgt cattgacgga aggctccagt aactgatcgc gggcgcagtc tgttctattg
     5941 tcaccagggt agtgtatcgg gtcgattcca caatcgtcag ccgatattgt gcgtttgcca
     6001 cctgatagct tacagtttcg ccgggtgcgt cattgcgcgg taacaaacgg cgcaattgtg
     6061 aaaagttcat ctcgcacagg cgcatcattt caggaaagtc aggtgtgtaa cgcttcattt
     6121 atgcccactc attttttaac gcttgatgat gcagctgcag ccattgcaaa gcgatgaccg
     6181 acgctgcgtt gtcgattttc ccctcttcta cccactggta tgcctgttcc cggcttacca
     6241 catgaacgcg aatatcttcg ttttcatcag ccagaccgtg aataccgctt gcggtcgtgg
     6301 cgtccacttc gcccaccata attgacgaac gctcactggt gccccccggg cttgccagga
     6361 aacttaacac cggtttggtc cgtttgacta tcagccccgc ctcttcaatc gcttcgcgac
     6421 gggcaacatc ttccacactt tcaccctctt caatcattcc ggcaaccatc tccagtagcc
     6481 aaggggtttc gctagtgtcg tatgcggcaa ttcgaatctg ctcaatcagc acaacttcat
     6541 cacgcactgg gtcaaagggt agcaagactg cggcgtgacc gcgctcaaaa atttcccgcc
     6601 gtacctcatg actcatttgc ccgttgaata gacgatgacg aaatctataa agatctaatg
     6661 aaaaaaagcc gcgataaagt gtttctcgtg caataatttc tacatcgttt ttgccaaatg
     6721 taacgggcag gttgtctggc ttaagcattg ttaatgtcct ggcactaata gtgaattaaa
     6781 tgtgaatttc agcgacgttt gactgccgtt tgagcagtca tgtgttaaat tgaggcacat
     6841 taacgcccta tggcacgtaa cgccaacctt ttgcggtagc ggcttctgct agaatccgca
     6901 ataattttac agtttgatcg cgctaaatac tgcttcacca caaggaatgc aaatgaagaa
     6961 attgctcccc attcttatcg gcctgagcct ttctgggttc agttcgttga gccaggccga
     7021 gaacctgatg caagtttatc agcaagcacg ccttagtaac ccggaattgc gtaagtctgc
     7081 cgccgatcgt gatgctgcct ttgaaaaaat taatgaagcg cgcagtccat tactgccaca
     7141 gctaggttta ggtgcagatt acacctatag caacggctac cgcgacgcga acggcatcaa
     7201 ctctaacgcg accagtgcgt ccctgcagtt aactcaatcc atttttgata tgtcgaaatg
     7261 gcgtgcgtta acgctgcagg aaaaagcagc agggattcag gacgtcacgt atcagaccga
     7321 tcagcaaacc ttgatcctca acaccgcgac cgcttatttc aacgtgttga atgctattga
     7381 cgttctttcc tatacacagg cgcaaaaaga agcgatctac cgtcaattag atcaaaccac
     7441 ccaacgtttt aacgtgggcc tggtagcgat caccgacgtg cagaacgccc gcgcgcagta
     7501 cgataccgtg ctggcgaacg aagtgaccgc acgtaataac cttgataacg cggtagagca
     7561 gctgcgccag atcaccggta actactatcc ggaactggcg gcgctgaatg tcgaaaactt
     7621 taaaaccgac aaaccacagc cggttaacgc gctgctgaaa gaagccgaaa aacgcaacct
     7681 gtcgctgtta caggcacgct tgagccagga cctggcgcgc gagcaaattc gccaggcgca
     7741 ggatggtcac ttaccgactc tggatttaac ggcttctagc gggatttctg acacctctta
     7801 tagcggttcg aaaacccgtg gtgccgctgg tacccagtat gacgatagca atatgggcca
     7861 gaacaaagtt ggcctgagct tctcgctgcc gatttatcag ggcggaatgg ttaactcgca
     7921 ggtgaaacag gcacagtaca actttgttgg tgccagcgag caactggaaa gcgcgcatcg
     7981 tagcgtcgtg cagaccgtac gttcctcttt caacaacatt aatgcttcta tcagtagtat
     8041 taacgcctac aaacaagccg tagtttccgc tcaaagctca ttagacgcga tggaagcggg
     8101 ctactcggtc ggtacgcgta ccattgttga tgtgttggat gcgaccacca cgctgtacaa
     8161 cgccaagcaa gagctggcga atgcgcgtta taactacctg attaatcagc tgaatattaa
     8221 gtcagccctg ggtacgttga acgagcagga tctgctggca ctgaacaatg cgctgagcaa
     8281 accggtttcc actaatccgg aaaacgttgc cccgcaaacg ccggaacaga atgctattgc
     8341 tgatggttat gcgcctgata gcccggcacc cgtcgttcag caaacatccg cacgcactac
     8401 caccagtaac ggtcataacc ctttccgtaa ctgatgacga cgacggggct tcggccccgt
     8461 ctgaacgtaa ggcaacgtaa agatacgggt tatctgccgc attcttcccc cttctcgctt
     8521 caatttcgac cagccatcct ctattctgat gggtatttac cacaggtccc ggaagacaaa
     8581 aatgaaacgg acaaaatcca tacgccacgc atcgttccgc aaaaactgga gcgcacgcca
     8641 tctgacacca gtcgctctcg cggttgccac tgtttttatg ctggctggct gtgaaaagag
     8701 tgatgaaaca gtgtctctct atcaaaatgc tgacgactgt tcagctgcaa acccaggcaa
     8761 aagcgccgaa tgtaccaccg cgtacaacaa tgcgctgaaa gaagccgaac gtactgcgcc
     8821 gaaatacgcc acccgtgaag actgtgttgc tgaatttggt gaaggtcagt gccagcaggc
     8881 accagcccag gctggcatgg caccagaaaa ccaggcgcag gcccagcaat ccagcgggag
     8941 tttctggatg ccgctgatgg ccggttacat gatgggtcgt ctgatgggcg gcggtgcggg
     9001 atttgcacag cagccgctgt tctcctcgaa aaacccagcc agcccggctt acggtaaata
     9061 taccgacgcg acgggtaaaa actatggcgc agcccagcca ggccgcacca tgaccgtacc
     9121 gaagacagcc atggcaccaa aaccggcgac caccactacc gttacccgtg gcggttttgg
     9181 tgaatctgtt gccaaacaaa gcactatgca gcgtagcgca accggtacct cttctcgttc
     9241 aatgggtggc tgataccgat ggaaagagtc agtattaccg agcgcccgga ctggcgtgag
     9301 aaagcccacg aatacggttt caattttcac accatgtacg gcgagccgta ctggtgtgaa
     9361 gatgcttact acaagttgac cctcgcccag gttgaaaagc tggaagaagt caccgccgaa
     9421 ctgcaccaga tgtgcctgaa agtggtggaa aaagtgatcg ccagcgatga gctgatgacc
     9481 aaattccgca ttccaaaaca cacctggagt tttgtgcgcc agtcatggct gacgcaccag
     9541 ccatcgcttt attcgcgtct tgatctggcg tgggatggca ctggcgaacc taaacttctg
     9601 gaaaataacg ccgatacgcc aacgtcacta tacgaggcgg cgttctttca gtggatctgg
     9661 ctggaagatc agcttaacgc cggtaacttg ccggagggca gcgaccagtt taacagtctg
     9721 caagaaaaac tgatcgatcg cttcgttgag ctgcgtgaac agtatggctt ccagttgctg
     9781 catctcacct gctgtcgcga cacggtggaa gatcgcggta ccattcagta tttgcaggac
     9841 tgcgcgacgg aagctgaaat cgctaccgag ttcctctaca ttgacgatat cgggttaggt
     9901 gaaaaaggtc agttcacgga tttacaggat caggtaattt ccaacctgtt caagctgtat
     9961 ccgtgggaat ttatgttgcg tgagatgttt tccaccaagc tggaggatgc aggcgtacgc
    10021 tggctggaac cggcgtggaa gagcattatc tccaacaagg cacttctacc gctactgtgg
    10081 gagatgttcc cgaatcaccc gaacctgctg cccgcttatt ttgcggaaga tgatcatccg
    10141 caaatggaaa aatatgtggt taaaccgatc ttctcccgtg aaggcgcaaa cgtgtcgatc
    10201 attgagaacg gcaaaaccat tgaagcagcg gaaggtccgt atggcgaaga agggatgatt
    10261 gttcagcaat tccacccgtt accgaaattc ggcgacagct atatgctgat tggtagctgg
    10321 ctggtgaacg atcaacccgc cggaattggc attcgtgaag accgtgcatt gatcacccag
    10381 gatatgtctc ggttttatcc acatattttt gttgaataat caatgatgcc ggatggcatt
    10441 cgccatccgg taattgttaa cctatctgca ccgacagcat acttaaactg cccatttcta
    10501 taccatcgac cggaatggta attggctcct gcccatccca cgcacctaag acatacagca
    10561 acggcagata gtgctctggc gttgggttcg ataacgcgcc accttcatgg tcgagataat
    10621 tcaccagagg atgctgctct accggccctt gccacgtcag attcgctttc acatactcat
    10681 taaacgacat cgcccaagga tacggtgaac tatcaccgtg ccacttcact gtacgcaggt
    10741 tatgcaccac gttgccgctg gcgaccaaca ttattccttc atcacgcagc gccgcgagtt
    10801 ttcgccccat ttcgaaatgc caggcggcag gtttgctact gtcgatactc aactgcacca
    10861 tcgggatatc cgcgtcagga tacatcttaa tcagcacgcc ccaggaaccg tggtcaaagc
    10921 cccaggcttc tttatccagc gtcaccggga tcggcgctaa cagctcaacc agacgctgtg
    10981 ccagcgcagg cgaacccgga gcaggataat gcgtatcgta cagcgcctgc gggaagccac
    11041 caaagtcatg aatcgtgggc ggcgtctcca tcgcggtcac tcctgttcca cgggtaaacc
    11101 agtgagccga aactaccaca atcgcttgcg ggcgtggcaa tgtcatcccc aacgtctgcc
    11161 agctgcgggt atacaaatta tcttccagca cgttcatcgg actaccgtga cctaaaaaca
    11221 atgctggcat acgtgttgaa gacatgatga tatccttaac taaaggtgtc attttgatat
    11281 cctcacgata cgcttgttcg gcggagtaag aacccggata acaatgatga tgatcatcag
    11341 ttattttgac gatctgcctg aaggtgaaga tttataagga gttgtcgatg tcagtacctc
    11401 tcattctgac catactggcg ggggcagcca cgtttattgg cgcgtttctc ggcgttctcg
    11461 ggcaaaaacc ctcgaaccgc ttactggcgt tttcgctagg atttgcggcg gggatcatgt
    11521 tgctcatctc attaatggaa atgcttcctg ccgcactagc agctgaagga atgtcgcctg
    11581 tgttgggtta tggaatgttt atcttcggtc tgcttggcta ttttggcctg gaccgcatgt
    11641 tgccgcatgc gcatccgcag gatttaatgc aaaaatcggt gcagccgttg ccaaaatcga
    11701 tcaagcgcac agccattctg ctcactctcg gcatcagtct gcataacttc ccggaaggga
    11761 ttgccacctt tgtcacggcg agcagcaacc tggagctggg atttggcatc gcactggccg
    11821 tcgcgttgca caatattcct gaaggtctgg cggttgcagg cccggtttat gcagcaacgg
    11881 gttctaaacg cacggcaatt ctgtgggcgg ggatttctgg actggcagaa attcttggtg
    11941 gtgtgctggc gtggttaatc ctcggtagca tgatttcccc ggtggtcatg gcagcaatca
    12001 tggcggcggt cgcaggaatt atggtggcgc tctcggttga tgaattaatg ccgctcgcca
    12061 aagagattga ccctaataat aaccccagct atggcgtact atgtggaatg tcagtgatgg
    12121 gattcagttt ggtgctgcta caaacggcgg gaattggtta aaagcaacat cagaagttgt
    12181 ccagcgcagg gacaacttct gctagtttca ctaaagcctt attctacctt ttcggctgtc
    12241 tcttctctcg tactgtttaa gtatttgttc cgcgcttcaa gcagtgcgtc tctgtcctgg
    12301 cgaaatgccc gacaataacg tttcaaatgc aggaaataac ccaccacaac cagaacaatc
    12361 aatacaatcc agtaccaggc gataaacatc cttttaccct taatatttaa caatactaaa
    12421 cat
//
LOCUS       DBFIFM010000132        12079 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.132, whole genome shotgun sequence.
ACCESSION   DBFIFM010000132 DBFIFM010000000
VERSION     DBFIFM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.132"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..122
                     /locus_tag="N7C74_003242"
     CDS             <1..122
                     /locus_tag="N7C74_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HGU2433060.1"
                     /translation="IESFGEMAYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            150..284
                     /locus_tag="N7C74_003243"
     CDS             150..284
                     /locus_tag="N7C74_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HGU2433061.1"
                     /translation="MADFIAQKIAELNKNTGKEVSEIRFTAREKMTGLESYDVKIKIM
                     "
     gene            complement(333..1406)
                     /gene="yccM"
                     /locus_tag="N7C74_003244"
     CDS             complement(333..1406)
                     /gene="yccM"
                     /locus_tag="N7C74_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGU2433062.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWHNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETAGSTIFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGALGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFTVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1478..4222)
                     /gene="torS"
                     /locus_tag="N7C74_003245"
     CDS             complement(1478..4222)
                     /gene="torS"
                     /locus_tag="N7C74_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HGU2433063.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQRLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFIQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAIGNAAQALETLQNSEPFAAALVDFDLPDVN
                     GITLARQLARQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQANNDLPLDVSQLNEDAQLMGTEKIHEWLALFTQHALPLLDEIDIARATQDSEKIK
                     RAAHQLKSSCSSLGMRSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLNKKDLNAI
                     "
     gene            4305..5333
                     /gene="torT"
                     /locus_tag="N7C74_003246"
     CDS             4305..5333
                     /gene="torT"
                     /locus_tag="N7C74_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HGU2433064.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5306..5998)
                     /gene="torR"
                     /locus_tag="N7C74_003247"
     CDS             complement(5306..5998)
                     /gene="torR"
                     /locus_tag="N7C74_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HGU2433065.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPYTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6128..7300
                     /gene="torC"
                     /locus_tag="N7C74_003248"
     CDS             6128..7300
                     /gene="torC"
                     /locus_tag="N7C74_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HGU2433066.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRAGANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7300..9846
                     /gene="torA"
                     /locus_tag="N7C74_003249"
     CDS             7300..9846
                     /gene="torA"
                     /locus_tag="N7C74_003249"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HGU2433067.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTSRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQTYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWHDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKCNGTVEQVTAFNGPVEMVAQCEYVPASQVKL"
     gene            9843..10442
                     /gene="torD"
                     /locus_tag="N7C74_003250"
     CDS             9843..10442
                     /gene="torD"
                     /locus_tag="N7C74_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HGU2433068.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVDELENRVATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPTDHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFVARCHQYDSFGFYAALSQLLLVLVEGDHQNR"
     gene            complement(10596..10901)
                     /gene="cbpM"
                     /locus_tag="N7C74_003251"
     CDS             complement(10596..10901)
                     /gene="cbpM"
                     /locus_tag="N7C74_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HGU2433069.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(10901..11821)
                     /gene="cbpA"
                     /locus_tag="N7C74_003252"
     CDS             complement(10901..11821)
                     /gene="cbpA"
                     /locus_tag="N7C74_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HGU2433070.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFSRQFQHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         2881 a         3116 c         3225 g         2857 t
ORIGIN      
        1 ttattgaatc tttcggggag atggcttata acatttctta cctgaccagg gtaccgggaa
       61 ccaacacctt actggcgtgt tgctgtcttt taagaccaga agaggttaac agtgaatatt
      121 gaagagttaa aaaacaagcc gaaacggaaa tcgccgactt tatcgcgcaa aaaatcgccg
      181 agctgaacaa gaatacaggg aaagaagtct ctgaaattcg cttcaccgcg cgagaaaaaa
      241 tgaccgggct tgaaagttat gatgtcaaaa tcaaaataat gtgattttgt gaacatcacc
      301 ccgtgcgagg tgatgttccg cttgttgcta atttagtgac caatcattgg cgcttgcgga
      361 attaagcgtc ggtacaattc ctccggcacc gggctttgcc atactcctgc atacattgcg
      421 taaccaatca ccgtaaacat aatccccaga accagtagcg tcattaacca gccagacaac
      481 gcaaaggctt ttttatttgc cgcaggtttt tgcagtgaaa aggtcaatgt tgaggctacc
      541 ggacatgact ctacgcaagt catacagccg gtacattcca ctgttcgtac ctgaattaat
      601 ttatcgaccg ggatccgtga tgggcaattt tttgcgcatt tgccacaatc gatacaactt
      661 tcggcattgc gacgaatctt aaacggcgat aatagcgaaa ccacgcccat cagcgcgcca
      721 tatgggcaaa gataacgaca ccaggcatgg cgaataaaca ggctggcaat cagcaaaacg
      781 gtcacgctga ttaatgtcgc ggtccccata tgacgaaaga aatcgagcat tttaacgtcc
      841 atcaccacgc tgtagggcga caacataaaa tagtgaatcg cctgagcagg catcaataac
      901 gcgatataga gaaaaaaact caacagcaaa tactttacac cgcgcagagg aatatccagc
      961 cagcggggaa ggacacattg ccgaccaaac agtttgttac cgagagcgcc gattaattca
     1021 gaaagcgtac caaccgggca taaccatgag caaaaggcct ttttgagtaa tagactgatg
     1081 acgataaaag cgaccaataa cagcatcgcg gcggcgtgga cggacggtaa ctgacctgtt
     1141 gcaaggctat atttcagatt catcagaccg gcaatcggta gccagccttc gatacctccc
     1201 ggtctggcga caaatatcgt actacctgcc gtttcgtaat agcgcaccca ataccagaac
     1261 gtgatggcaa tataaatatt cattgccaac agcaataatt gcgtcgcttt acgccaggtc
     1321 gtggcattgt gccagtcatt ccacggtaat ttgccgcccg tcgtgcctgg ccgccgctgc
     1381 cagcgggttc tttttttctc tgccatgatt ttgccagtcc gttaagttgc ataccagatg
     1441 ccactattct agttattctt aactggctga tattgattca aatcgcgttc aggtctttct
     1501 tattcaacca tgcttccaga gcggcaacac tgcgtgtaat ttcttcgtgt ggaagggggg
     1561 cagataatgg ctgctgctcc agttgtgcgc agagctggct ggcgctacgc attcccagac
     1621 tcgagcaact gctttttagc tgatgcgcgg cacgctttat tttctcgcta tcctgagtgg
     1681 cgcgggcaat gtcgatttca tcgagaagcg gcagggcatg ttgtgtaaat aatgccagcc
     1741 attcgtggat cttctccgtc cccattaact gagcgtcttc attgagttgc gatacatcca
     1801 gtggtagatc gttattggct tgcagttgga gatagtgcgc cagtaactga ccgagcactt
     1861 cacgcggcac cggtttaggg ataatcccgc gaaatagtga actggtacgc tggcgcagcg
     1921 tttcgtcaat gacatgggcg ctaaagccaa tcaaaaccag cgacggatat tgtcgtgcca
     1981 gttgtcgggc aagcgtaatg ccgttgacat ccggcagatc aaaatccacc agtgcggcag
     2041 caaacggttc gctattttgc agtgtctcta aagcctgcgc ggcattgcca atagcaacaa
     2101 cctgcgcacc actggtgttc agcatctcaa cggtaattcg ctgggttagc gggttatctt
     2161 caattaacag caaacgtaaa ccgtcaagac gcaccgcctg attgactgtt tttggcacgg
     2221 gtgccgtggc aacacgtaac ggcaagcgta aacaaaaaca gcttccaacc tccggcgtgc
     2281 tggtggcgct cagttcgccg cccatcgcct gggccagacg gctactgata gtcagcccca
     2341 gcccggtgcc gccgcgtttg ccacttacct gaataaatgg ctggaagatt tctgccagtt
     2401 tcgcgggatc aataccgcag ccgctgtctt ccacttcgac cagccattgc tcaccatcag
     2461 tacggctacg taaaacgatc tgcccttcgt cagtaaaacg cagggcgttg ctcaacaggt
     2521 tggtaatgac ctgacgaata cgccgcggat cgcccattaa cgcagtcggt acatcgtcgg
     2581 caattgccgt tgccaggcga atcgggcgac ctttcacccg tccactcatt aattgcaggg
     2641 tactttccag cagcggtcgc ggttcaaagg gttcatcgct gaccgaaaca ttcttgccac
     2701 ctgcttcgat agcggaataa tcgagaatat cgttgaggat ggtcagcaac gattcgccgg
     2761 agtcggtaat tgcccgcaaa tcatcacgct gggcgttaag tgcggggtta tctgccagta
     2821 gctgtgcggt gccgagaata ccgtacagcg gtgtgcggat ctcatggctc atcgccgcca
     2881 gaaacgccga ttttgcctgg ctggcttttt ctgcttccgc ccgtgcttgt cggtgttcta
     2941 tcaccagttc ctgcaattca gctgtacgcg ctttgacctg cgccgccagc tgttcgcggt
     3001 ggcgattcag tgcatgaaca ctgctgcgaa acgcatccat cagccgcccg atggtgtcca
     3061 gctcccgtac gccagcggtt tccgggaaag gggagtcaat atcaccgtcc agcagccgtt
     3121 gcagcgcctg cgtttgttcg gcaagtggac gcgtgactga gcgataaacc acgcgccaga
     3181 ggatcagaat cagtgcgcaa agtgaaacca tccccagcaa taacaggctg tattgcccgc
     3241 gtgcactggc tttttccaga tgcgccagtc cgtgctgatt acgcagctca atggtgtcga
     3301 ccagctgact gacttcgctg ctaaactgcg cgaactgggc gatgttattt tgtgcgagag
     3361 tttgtaggtg attgctgatt tcactgtcct gctggaacag cgccagcaaa tcgctatatt
     3421 ggctaacggt agttaacgtt gttgcgacct gagcacgaac tcccggatct tcaatgcgta
     3481 tttgccgacg ttgcagaatt ttcaccgcat tattgagctg cttttccagc gttggcgcat
     3541 ttttctggat ctgctccagc cccagattca tcaccatttg ctgcacccgc agagcgctaa
     3601 ggcgcagttc attcatctgg ttaacatact caagatcgat atcaatcagc cgatcgagtg
     3661 cactttcagc agcctgacgc tgatcttgtt cgatcaaatc gtaaatcccg gcctgggtcg
     3721 ctccggcgga ggttgccgca ttattcgcct gaccttgcgc caggcgtgcg atctcatcgg
     3781 cggcagcgac tatctgctga ctgagtcgct gttgttgctg gcgcagttgc agacgccgcc
     3841 ccaccagttc cccttgctga cgtaacgaac gggagatctc ctgctcctgt tgttcaatag
     3901 cggtggtatc aaaaccttgt tcccgtaacg cttgcagcaa cgcattaatc ttcaggcttt
     3961 gtgcggtgag cattcgcccc tgcgcctgcc acatcttttc gttatcggca ctggtcaggt
     4021 tctgcgcggc gaaaagttcc caggcgctgg cttcgctcaa ctggcgcgcc atattcatgg
     4081 taggaatcaa tgcctgagta ttgtcttttt ccacctggct gataaagcgc aggttgtacc
     4141 atcccaccag ggtactggtc agggttaaca gcgccatcag ggcaaagccc atccagagtc
     4201 ttcgggtcag ggttaaattc acggtcggtg cacttaaggt gaaaaatgtt gagtcgcaaa
     4261 gcggaatgca tctagcataa agccttatta ttgatgaggc tatcatgcgc gtgctgctat
     4321 ttttacttct ttcccttttc atgttgccgg cattttcggc tgataacctg ttgcgctggc
     4381 atgatgcgca gcatttcacg gtgcaagcct ctatgccgct aaaagccaaa cgcgtatgga
     4441 aactgtgcgc gctttatccc agcctgaaag attcatattg gttatcgttg aactatggta
     4501 tgcaggaggc tgctcgccgc tacggtgtgg atttaaaagt gctggaggca ggcggctaca
     4561 gccagttggc tacccagcaa gcacaaatcg accagtgtaa acagtggggc gcagaggcca
     4621 ttttgctcgg tagtagcacg acgtcatttc ccgatctgca aaagcaagta gcaaatctgc
     4681 cggtgatcga gctggtaaat gctattgatg ctccccaggt gaaaagccgc gttggtgtgc
     4741 cctggtttca gatgggctat caacctgggc gatatctggt gcaatgggcg cacggtaaac
     4801 cactgaatgt gctgttgatg cccggacccg ataacgccgg gggcagtaag gagatggtcg
     4861 agggttttcg cgcagccatt gccggaagcc cggtgcgtat tgttgatatt gcgcttggtg
     4921 ataacgatat tgaaatccag cgtaacctgt tgcaggagat gctggaacgc catccagaaa
     4981 tcgacgtcgt tgccggaacg gccattgcgg cagaggcggc aatgggggaa gggcgtaacc
     5041 tgaaaacgcc gcttaccgtg gtgtcgtttt atctttcaca tcaggtgtat cgcgggctga
     5101 agcggggaag agtgattatg gctgccagcg atcaaatggt ctggcagggg gaactggcag
     5161 ttgagcaggc catcaggcaa ttacaggggc aatcggtgtc tgataatgtc agcccaccga
     5221 ttttagttct gacgccgaaa aatgccgacc gcgaacatat ccgccgctcg ctgtcgcctg
     5281 ggggatttcg tccggtctat ttttatcagc acacatcagc ggctaagaaa taaccttcgc
     5341 catgttgcgt caccagtaaa tccgcgctga gtttatggcg taaacgacga attaacacat
     5401 cgacggtgcg caggtcaggg ttttccaccc gacgcgcaga gagcatacgc agcagacgtt
     5461 cacggctgag aatttcgccc ggattggtca caaatgccac caacatttca tactctgcgc
     5521 gggtcagttt aatcggctca ccatcccgct ccagcgtatg gcgcgacaca ttcaggcaat
     5581 aacccgcaaa gcgatagcag ttgtcctgag tgtacggttg agcttgtcgc gcgaggtcga
     5641 ttcgccagag cagatttttt acccgtacta ccagttcgcg cagttccagc ggtttggtga
     5701 cgtaatcgtc tgcgcccatt tccagcccaa caatacggtc aatccgatcg ctgcgtccgg
     5761 taaccagaat aatccccacc gttgagcgtt ctcgcagggc gcgggttaac atcaggccat
     5821 tttcatcggg caagttgata tccagcagaa ttaaatccac cggctgattc tgcataattt
     5881 cccgtagccc ggcaccgctc gccgtaacgg aaacggtata cccctcctga gtgaagtagg
     5941 attgtaatcg cgcctgggta accggctcat cttcaacaat aacaatgtga tgtggcatca
     6001 gagggtttta ctcattctgt tcatatctgt tcatattctg ccgtaagccg ttcatcctga
     6061 ccagtgccgc tgttcatatt tactcattaa gatcgcttca ctaaaccata attttacagg
     6121 ggctattatg cggaaactct ggaacgcgct acgccgaccc agtgctcgtt ggtcggtact
     6181 ggcgctggtc gctattggga ttgtgattgg cattgcgctg attgtattgc cacacgttgg
     6241 gatcaaagtc accagcacaa ccgaattttg tgtcagttgc cacagtatgc aaccagtgta
     6301 tgaagaatat aaacagtcgg tgcatttcca gaacgcctcc ggcgtgcgag ctgaatgcca
     6361 tgactgtcat atcccgccgg atattccagg catggtgaag cgcaaactgg aagcgagcaa
     6421 cgatatctac cagaccttta ttgcccactc cattgataca cctgaaaaat ttgaagccaa
     6481 acgcgcggaa cttgccgagc gtgaatgggc gcgaatgaaa gaaaacaact cggcaacctg
     6541 tcgctcctgc cataactacg atgcgatgga tcatgcgaag cagcatcctg aagcggcacg
     6601 tcagatgaag gtggcagcga aagataatca atcctgcatc gactgtcata aaggtattgc
     6661 ccaccagtta ccggatatga gtagcggctt ccgtaagcag ttcgatgagc tgcgcgccgg
     6721 tgctaatgac agtggtgaca cgctgtactc tattgatatt aagccaattt atgcggcgaa
     6781 aggcgataaa gaagcctctg gttctctgct gcctgcctcg gaagtgaaag tccttaaacg
     6841 tgacggcgac tggctgcaaa ttgaaattac cggctggaca gaaagcgccg gacgtcagcg
     6901 tgtactcacc caattcccag gtaaacgcat ctttgttgcc tcgattcgtg gtgatgtgca
     6961 gcagcaggta aaaacgctgg agaaaaccac cgttgccgac accaataccg agtggagcaa
     7021 gttgcaggcc accgcgtgga tgaagaaagg tgacatggtg aacgatatca aaccgatctg
     7081 ggcttatgcg gattcgttgt acaacggcac ctgtaaccag tgccacggcg caccggaaat
     7141 ctctcacttt gacgctaacg gctggattgg cacgctcaac ggcatgatcg gctttaccag
     7201 cctcgataaa cgtgaagaac gcaccttgtt gaaatatctg caaatgaatg cgtctgacac
     7261 cgcaggtaag gctcacggcg ataagaagga agaaaaataa tgaacaataa cgatctcttt
     7321 caggcatcac gtcggcgttt tctggcacaa ctcggcggct taaccgtcgc cgggatgctg
     7381 gggccgtcat tgttaacgtc gcgccgtgcg actgcggcgc aagcggcgac tgaggctgtc
     7441 atctcgaaag agggcattct taccgggtcg cactgggggg ctatccgcgc gacggtgaag
     7501 gatggtcgct ttgtggcggc aaaaccgttc gaactggata aatatccgtc gaaaatgatt
     7561 gccggattgc cggatcatgt acacaacgcg gcgcgtattc gttatccgat ggtacgcgtg
     7621 gactggctgc gtaagcgcca tctgagcgac acctcccagc gcggtgataa ccgttttgtg
     7681 cgtgtgagct gggatgaagc cctcgacatg ttctatgaag aactggaacg cgtacagaaa
     7741 actcacgggc cgagtgcctt gctgaccgcc agtggttggc aatcgacggg gatgttccat
     7801 aacgcttcgg ggatgctggc gaaagctatt gccttgcatg gtaatagcgt tggtacgggc
     7861 ggagattact ctactggtgc tgcgcaggtg atcctgccgc gcgtagtcgg ttcgatggaa
     7921 gtgtatgaac agcaaacctc ctggccgctg gtattgcaga acagcaaaac cattgtgctg
     7981 tggggctccg atttgctgaa aaaccagcaa gcgaactggt ggtgcccgga tcacgatgtt
     8041 tatgaatatt acgcgcagtt gaaagcgaaa gtcgccgccg gtgaaattga ggtcattagc
     8101 atcgatccgg ttgttacatc cacccatgag tatctggggc gcgagcatgt gaagcacatt
     8161 gcggttaacc cgcaaactga cgtgccgctg caactggcgc tggcgcatac gttgtacagt
     8221 gaaaacctgt acgacaaaaa cttcctcgct aactactgtg tgggttttga gcagttcctg
     8281 ccgtatctgc tgggtgagaa agacggtcag ccgaaagatg ccgcatgggc tgaaaaactg
     8341 accggcattg atgccgaaac cattcgtgga ctggcgcggc agatggcggc gaacagaacg
     8401 cagattattg ctggctggtg cgtacagcgt atgcagcacg gtgaacagtg ggcgtggatg
     8461 attgtggttc tggcggcgat gctggggcaa attggcctgc caggtggtgg ctttggtttt
     8521 ggctggcact ataacggcgc aggcacgccg gggcgtaaag gcgttattct gagtggtttc
     8581 tccggctcta cgtcgattcc gcctgttcac gacaacagtg attacaaagg ctacagcagc
     8641 accattccga ttgcccgttt tatcgatgcg atcctcgaac cggggaaagt aatcaactgg
     8701 aacggtaaat cggtaaaact gccgccgctg aaaatgtgta tttttgccgg aactaacccc
     8761 ttccatcgcc atcagcagat caaccgcatt attgaaggct ggcgcaaact ggaaacggtt
     8821 atcgccatag ataaccagtg gacctcaacc tgccgctttg ccgatatcgt gctgcctgcg
     8881 accacgcagt ttgagcgtaa cgatctcgac cagtacggca accactccaa ccgtggcatt
     8941 atcgccatga aacaggtcgt gccgccgcag ttcgaggcgc gcaacgactt tgatattttc
     9001 cgcgagctgt gccgccgctt taatcgcgaa gaagccttta ccgaagggct ggacgaaatg
     9061 ggctggctga aacgcatctg gcaggaaggt gttcagcaag gcaaaggacg cggcgttcat
     9121 ttgccagcgt ttgatgactt ctggaataac aaagagtacg tcgagtttga ccatccgcag
     9181 atgtttgttc gccaccaggc attccgcgaa gatccagatc tcgaaccgct gggcacgccg
     9241 agtggcctga ttgagatcta ttcgaaaacc atcgccgata tgaactacga cgattgtcag
     9301 gggcatccga tgtggtttga gaaaatcgaa cgctcccacg gtgggcccgg ctcgcagacg
     9361 tatccgttgc atctgcaatc tgtgcatccg gatttccgac ttcactcgca gttatgtgag
     9421 tcggaaactc tgcgtcagca atatacggta gcgggtaaag agccagtgtt cattaacccg
     9481 caggatgcca gcgcgcgcgg tattcgtaac ggtgatgtgg tacgcgtctt taacgctcgc
     9541 ggtcaggtgt tggcaggggc agtagtttct gaccgctatg cacccggcgt ggcgcgaatt
     9601 cacgaagggg catggcacga tccagataaa ggcggcgagc ctggtgcgct gtgcaaatac
     9661 ggtaacccca acgtgttgac cattgacatc ggtacttcgc agctggcgca ggcgaccagt
     9721 gcgcacacta cgctggtgga aattgagaag tgcaacggaa cagtggagca ggtaacggcg
     9781 tttaacggcc ccgtggagat ggtggcgcag tgcgaatatg ttcccgcgtc gcaggtgaaa
     9841 ttatgaccac gctgacagca caacagatag cctgtgttta cgcctggctg gcgcagttgt
     9901 tctcccgtga actggacgat gaacaactga cgcaaatcgc cagtgcgcag atggctgaat
     9961 ggttttcgtt gctgaaaagc gaaccgccgc tcactgcggc ggtggatgag ctggaaaacc
    10021 gtgttgccac tctgacagta cgtgacgatg cccgtctgga actggccgct gacttttgcg
    10081 gcctgtttct gatgactgac aaacaagcgg cgctgccgta tgcatcggcc tacaaacagg
    10141 acgagcaaga gattaaacgc ttgttagttg aggcagggat ggaaaccagc ggcaatttca
    10201 acgaaccgac agatcatctg gcgatctatc tcgagttgtt gagtcatctg catttttcgc
    10261 tgggagaggg gacggttcct gcgcgaagaa tcgacagttt acggcaaaaa acactcacgg
    10321 cgttgcggca atggttacca gagtttgttg cgcgttgcca tcagtatgac agctttggtt
    10381 tttacgcggc actaagccag ttattgctgg tgttagttga gggcgaccac caaaacagat
    10441 aacgtcgttt gttagcctga taagacgcgt ttagcgtcgc atcaggcatt agggcaccgc
    10501 cgccggatgc ggcgtgaaca cgccttatcc ggcccacagg aaaagtactc tttgcaggca
    10561 ggctaagatg cgtcatcaca tccggcaaac acacatcacg gatgagctac aaaccgggaa
    10621 agccgctggc gcagcaggcg gttttcctgc ttcaggtgcg caatatcatc cattaacgtc
    10681 agcgccaccg cgatccccgg ccagtccaga gccagttcat gacgcaggcg caccgcgcgt
    10741 tgcaccacga tggcggcatg gtcgtcaaat acccaggttg tttcctggat ctcacgcggt
    10801 tcaaccaccc ccaaaccgac aatttcattc aattcctctt cagagatgcc ggtatgcagg
    10861 caaaattcgg taatagtaaa agtcaccgta acattagcca ttatgctttc ccccaatctt
    10921 tacgtggatc aaaagacgac tgggcgtctg ccagttgctg ccacagcgcg gcagtgtttt
    10981 catccggttt cggcggcatc acgattttca gtaccgcata cagatcgccg gtctgttttt
    11041 tgctcaccag acctttgcct ttaacgcgca atcgttgccc ggcctggctg ccaggcggga
    11101 tagtcagcaa aatgctttct ttcagtgttg gaacggtgac tttagcaccc agcgccgctt
    11161 cccacgggct aaccggcacc acaatttcca gatcctggcc gacaatatca aacagcggat
    11221 gtggcgcaat atgaatcacc agccacaaat cgccatttgg accgccgttt tcgcccggcg
    11281 tcccctggcc tttcaggcgg atgcgttgac cattgccgac gcctgccggg atcttcacat
    11341 tcagcgtttt cgggatttcc tgttcgatca tgccaaaggc gttataaacc ggcaggttat
    11401 agctgatggt acgcttatgc tcagtaagcg tttcttcgag gaataccgcc acttcgattt
    11461 caatatcgtg gccgcgtgcg gcggggcgtt gacggctctg gcgggcatgc tgaccgaaaa
    11521 ttgacgagaa gatatcgtca aaatcttcgg cgttaaaact ctgaccgtcg ccatgctgga
    11581 actgacggct aaattgcgga tcgttgcgat gttgccacat ctgatcatac tcagcgcgac
    11641 gttgttcatc acttaacact tcccaggctt cagcgacctc tttgaagcgg gcttcggcat
    11701 ccggttcttt gctgacatca ggatggtatt tgcgggcaag tcgacgatag gcggtcttga
    11761 ttgtcttgag atcgtccgtc ggtttcacgc ccatgatggc gtaataatcc tttaattcca
    11821 tagcgttatc tcgcgtaaat caacacaaat tgaaggaacc cctgtaaggt aactcctata
    11881 agtgtagggt aatcctcaaa atttcatatg ccaacacaga atatgttatt gaaatcatcg
    11941 cggagaggag gtcgcctcaa ggtggggcgc tgaacatatt ttaaacaggt gaaaaagggt
    12001 gagcgatttt tgatagttga aacaggcact ttaagtttta ctagtgcgtt attatttatt
    12061 aaattttata ggcgctata
//
LOCUS       DBFIFM010000133        12004 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.133, whole genome shotgun sequence.
ACCESSION   DBFIFM010000133 DBFIFM010000000
VERSION     DBFIFM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.133"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            332..625
                     /locus_tag="N7C74_003253"
                     /pseudo
     CDS             332..625
                     /locus_tag="N7C74_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(683..1114)
                     /gene="tnpA"
                     /locus_tag="N7C74_003254"
     CDS             complement(683..1114)
                     /gene="tnpA"
                     /locus_tag="N7C74_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS609 family
                     transposase"
                     /protein_id="HGU2433071.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDATEK
                     LRTYFSKVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISAIRQYIEQQQTPG"
     gene            1122..2330
                     /locus_tag="N7C74_003255"
     CDS             1122..2330
                     /locus_tag="N7C74_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HGU2433072.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTASQSCGKWY
                     ISIQTENEVSTPVHPSALMVGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQACGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            2374..2481
                     /locus_tag="N7C74_003256"
                     /pseudo
     CDS             2374..2481
                     /locus_tag="N7C74_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(2465..3103)
                     /gene="ytfB"
                     /locus_tag="N7C74_003257"
     CDS             complement(2465..3103)
                     /gene="ytfB"
                     /locus_tag="N7C74_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HGU2433073.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNYPDQVAPVAPEPIQ
                     EGQPEEQPKTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            3321..3941
                     /gene="fklB"
                     /locus_tag="N7C74_003258"
     CDS             3321..3941
                     /gene="fklB"
                     /locus_tag="N7C74_003258"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU2433074.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            4250..5662
                     /gene="cycA"
                     /locus_tag="N7C74_003259"
                     /gene_synonym="dagA"
     CDS             4250..5662
                     /gene="cycA"
                     /locus_tag="N7C74_003259"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HGU2433075.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(5707..6369)
                     /gene="ytfE"
                     /locus_tag="N7C74_003260"
     CDS             complement(5707..6369)
                     /gene="ytfE"
                     /locus_tag="N7C74_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HGU2433076.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHARHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHNTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6477..7442)
                     /gene="ytfF"
                     /locus_tag="N7C74_003261"
     CDS             complement(6477..7442)
                     /gene="ytfF"
                     /locus_tag="N7C74_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU2433077.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPNFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(7550..8410)
                     /gene="qorB"
                     /locus_tag="N7C74_003262"
     CDS             complement(7550..8410)
                     /gene="qorB"
                     /locus_tag="N7C74_003262"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313217.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HGU2433078.1"
                     /translation="MIAITGATGQLGQYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKTAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGHPTTTLAE
                     SVSHLFNVNN"
     gene            8499..8879
                     /gene="ytfH"
                     /locus_tag="N7C74_003263"
     CDS             8499..8879
                     /gene="ytfH"
                     /locus_tag="N7C74_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HGU2433079.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(8997..10940)
                     /gene="cpdB"
                     /locus_tag="N7C74_003264"
     CDS             complement(8997..10940)
                     /gene="cpdB"
                     /locus_tag="N7C74_003264"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HGU2433080.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINNARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYEPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            11130..11870
                     /gene="cysQ"
                     /locus_tag="N7C74_003265"
     CDS             11130..11870
                     /gene="cysQ"
                     /locus_tag="N7C74_003265"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HGU2433081.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGAKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2876 a         2964 c         3112 g         3052 t
ORIGIN      
        1 ggtggacgaa aaacgagata aatatgcagg aaatgaataa atagtggttg gtaatgtcat
       61 tgtaatgtga cctgtttaat gtttttataa atatcgcagc tgaatgttca cagaaaaaaa
      121 tcgatattaa taaaaataag tttgttttct gaagtggtga aaatagcgga tggtgaaaga
      181 tattaatatc aaaaatcgct cattatggat ataacgatgc acccggggag aaaatgactt
      241 ccacgcctta caccgtacat tcttgttgtg cggtatgtaa ttattctacg ctgtcggtat
      301 cggtgtattc gttaatcagc aggttaacca gccacaaaaa gccattgaga aaattattga
      361 ttttacatgg gattattata ttgctaaccc ctggttttta aaaattgtgc atagtgaaaa
      421 tcaaagtaaa ggtgtgcatt atgcaaaatc acagcgtctc ttagaaatca accatgcgca
      481 cttgcagtta atggaatcct tattggatga agggaagaaa tacaatattt ttaagccaga
      541 tatcgaccca ttgcaggtga atattaatat tgctgcgctt gggggatatt atttgatcaa
      601 ccagcatacg cttggcctgg tttatccggt gcgccgtaaa accccgtcct tcagggcggg
      661 gatatacggc gcggttttcc acctaaccag gtgtttgctg ttgttcaatg tattgacgga
      721 tggcggatat tggcgcacct ccgcagctac tggcaaaata gccaggactc cacaaaacgc
      781 ctttgtagta atacctgact gcaatatctg gtcgatctcg tcgcagtaac ctacccgata
      841 cgcctttgag gctgtttacc agactggata tcgccagttt gggaggatag ttaattaaca
      901 aatggacgtg atctggttcg ccatccattt caaccagttc agcttcaaaa tcagcacata
      961 cctttgaaaa gtaagtgcgt agtttttctg tcgcgtcatg gtcaaaaatc tggcgtcggt
     1021 atctggtgac aaagaccagg tgaacatgca tcaggaaaac acaatgcctt ccacgccgga
     1081 tatcggtttc ttttttcaca gaccaaagta tcatttggcc tgtgaaacga ctacaggcat
     1141 ttaaattcca gttaagaccc ggtggtcaac aggagcgtga aatgaggcgc ttcgcaggcg
     1201 catgtcgttt cgttttcaat cgtgctctgg cgcttcagaa tgagaatcat gaggccggta
     1261 ataaatacat cccttacggg aaaatggctt cctggctggt tgagtggaaa aatgccactg
     1321 aaacgcaatg gcttaaagat gccccgtcac agccattgca acagtcactg aaagaccttg
     1381 agcgggctta caaaaacttc ttccggaagc gggctgcttt tccccgattc aaaaagcggg
     1441 gacagaatga tgcattccgc tacccgcagg gcgttaagct cgatcaggaa aacagccgta
     1501 tttttctgcc gaaacttggc tggatgcgct accggaatag ccgtcaggtc acgggtgttg
     1561 tgaaaaatgt cactgccagc cagtcctgcg gtaagtggta catcagtatt cagactgaaa
     1621 atgaagtatc aactccggtt cacccttcag cattaatggt cggactggat gctggcgtgg
     1681 ctaaactcgc cacgctgtca gatggcacag tctttggacc tgtaaacagt tttcagaaaa
     1741 accagaagac gctggcgaga cttcagcgac agttaagccg caaggtcaaa ttcagcaaca
     1801 actggcagaa gcagaaacgc aaaatacagc gactgcattc ctgtatcgca aatatccgca
     1861 gggactacct tcacaaagtc acaacgaccg tcagcaaaaa ccacgcaatg atagtcattg
     1921 aggatttgaa ggtcagcaac atgtcaaagt cagcagcggg tacggtcagc cagccggggc
     1981 gcaatgtccg ggcaaaatca ggtttaaacc gttcgatact ggatcagggc tggtatgaaa
     2041 tgcgccgcca gcttgagtac aagcagctct ggcgtggcgg tcaggtgctt gctgttccgc
     2101 cagcgtacac aagccagcgt tgcgcgtgct gtggtcatac agcgaaagaa aatcgcctgt
     2161 cacaaagtaa attcagatgc caggcatgtg gatatacagc gaacgctgat gtaaatggcg
     2221 ctcgtaacat tttagcggcg gggcacgccg ttcttgcctg tggggagatg gtgcagtcag
     2281 gccgcccgtt gaagcaggaa cccaccgaaa tgattcaggc gacagcctga acgtagcagg
     2341 gatccacgtc cttcagggct tggaggatgt caacacatca gtatggtttc accccaggca
     2401 ctggaagcca gacgtaaggt catcaaagag acaatcctta gctggctttt ggttgaccct
     2461 tcatctaccg cgcacgaata aaactgccat ccggctggcg ggtgaacagg acctgttgat
     2521 tattccccgt atcaatggtt aagcccgtca ccacgccgct ggcgttctgg cggattttca
     2581 ccatctgccc gttttgcaaa ttactcagcg gcttacccgc gccttctacc tgcgccattg
     2641 catagacatc ggtagcgggc agtccgtggt cgcggaacag ctgcgccatc gttttaccgg
     2701 gttcgacacg gtaagagcgc cattgattat caattccgct atctggctgg aatggttgcg
     2761 tttgggtagt tttcggctgt tcttctggct ggccttcctg tataggttct ggagcgaccg
     2821 gagccacctg atcaggataa ttttgtgggg tgactaattg cgcccgcagc tgttcttctg
     2881 tcggcggttg tgattgcgac tgaatatcta actgcgcttc acgcgtcacc accggcgcgt
     2941 tgggcgtatc atcagatgga agcagaaatc cgaccaccaa tacaatggcg gcaataatga
     3001 tcccccgacg atgcatcggc ggcagcgggt ccatgaagcg aaaattgtcc ggtgcgtgcc
     3061 agactttctc cagggttggt tttagttcaa agcgcccggg catggtttcc tcctgctccg
     3121 cgtcttgttc ctcaatcata gcctatgaat aagctaacgc tatgatgtcc gtggtaaacc
     3181 cgcctttatt atattcatac gggatattgc tattgtttct ttttccctgg gatttgtcat
     3241 cattcccgcg acaaagtttt acccaaagaa gtgtggctga tatgctgccc gctactttat
     3301 accctaagaa aggaaatacg atgaccaccc caacttttga caccatcgaa gcgcaagcaa
     3361 gctacggcat tggtttgcag gtagggcaac aactgagtga atctggcctg gaagggctgc
     3421 tgccagaagc actggttgca ggtattgccg atgcgctgga aggcaaacat ccggctgttc
     3481 cggttgatgt ggtgcatcgc gcgctgcgtg aaatccacga gcgcgccgat gccgttcgtc
     3541 gtcagcgttt ccaggcgatg gctgctgaag gtgtgaaata cctggaagaa aacgccaaaa
     3601 aagaaggtgt gaatagcacc gaatctggcc tgcaattccg cgtgatcaac cagggtgaag
     3661 gcgcaattcc ggcacgtacc gaccgcgttc gtgttcatta caccggtaaa ctgatcgacg
     3721 gcaccgtgtt tgacagctcc gttgctcgtg gtgaacccgc tgaatttccg gttaatggcg
     3781 tgatccctgg ctggattgaa gcactgactc tgatgccggt aggttctaaa tgggaactga
     3841 ctatcccgca ggaactggca tatggcgagc gcggcgcagg cgcatccatc cctccgttca
     3901 gcaccctggt gtttgaagtc gaactgctgg aaatcctcta agcagcgcat tctgttcccc
     3961 tcgaaccaga ggggagcagg cattcagcaa taaacccttc agtttgccaa acggcgctat
     4021 tttgtgttgc aaagaccccg taagcgtgta tttttgtgag ctgtttcgcg ttatcaccgt
     4081 gatatgacac tcactttaaa cataaaatta acattagatc taaatcttag tattcatccc
     4141 gcgtattgtt acctaatatc gatgagtccc gatacagatt cgtcgtatca tagactgact
     4201 aaaggccgta gagcctgaac aacacagaca ggtacaggaa gaaaaaaaca tggtagatca
     4261 ggtaaaagtc gttgccgatg atcaggctcc ggctgaacag tcgctacggc gcaatctcac
     4321 aaaccgacat attcagctta ttgccattgg tggtgccatt ggtacggggt tgtttatggg
     4381 gtctggcaaa acgattagcc ttgccgggcc gtcgatcatt ttcgtttata tgatcattgg
     4441 ttttatgctc tttttcgtga tgcgggcaat gggggaattg ctgctttcga atctggaata
     4501 caaatctttt agtgacttcg cttccgattt actcgggccg tgggcaggat atttcaccgg
     4561 ctggacttac tggttctgct gggttgtaac cggtatggca gacgtggtgg cgatcacggc
     4621 ttatgctcag ttctggttcc ccgatctctc cgactgggtc gcctcgctgg cggtgatagt
     4681 gctgctgctg acgcttaacc tcgccactgt gaaaatgttc ggtgagatgg agttctggtt
     4741 tgcgatgatc aaaatcgtcg ccattgtgtc gctgattgtc gtcggcctgg tcatggtggc
     4801 gatgcacttt cagtcaccga ctggtgtgga agcgtcattc gcgcatttgt ggaatgacgg
     4861 cggctggttc ccgaaaggtt taagtggctt ctttgccgga ttccagatag cggttttcgc
     4921 tttcgtgggg attgagctgg taggtacaac agctgcggaa accaaagatc cagagaaatc
     4981 actgccacgc gcgattaact ccattccgat ccgtatcatt atgttctacg tcttcgcgct
     5041 gattgttatt atgtccgtga cgccgtggag ttcggtagtc ccggagaaaa gcccgtttgt
     5101 tgaactgttt gtgttggtag gtttgcctgc ggctgccagc gtgatcaact ttgtggtgct
     5161 gacctctgcg gcgtcttccg ctaacagtgg cgtcttctct accagccgta tgctgtttgg
     5221 tctggcgcag gaaggtgtgg caccgaaagc gttcgctaaa ctttctaagc gcgcagtacc
     5281 cgcgaaaggg ttgactttct cgtgtatctg tctgctcggc ggcgtggtga tgttgtatgt
     5341 gaatcctagc gtgattggcg cgttcacgat gattacaacc gtttccgcga ttctgtttat
     5401 gttcgtctgg acgattatcc tttgctcgta ccttgtgtat cgcaaacagc gtcctcatct
     5461 acatgagaag tcgatctaca agatgccgct cggcaagctg atgtgctggg tatgtatggc
     5521 gttctttgtg ttcgtggtcg tgttgctgac actggaagat gacactcgcc aggcgctgct
     5581 ggttaccccg ctgtggttta tcgcgctggg gctgggctgg ttgtttattg gtaagaagcg
     5641 ggctgctgaa ctgcggaaat aaccgcatta tcatgctgga tggcgcaatg ccatccagct
     5701 tttagatcac tcacccgcca gcgcccttgg gaacagtaca ttgttttcca ggctgatgtg
     5761 atccatcagg tcatcaatca gttcattaat gccgttatac atcgctttcc aggtggtgca
     5821 ggcttccggc ggcggtgtga cgttattggt attgtgtttg atcacttcca gcagttcacc
     5881 cgcttcatcg tgctcgcttt ccattacgct gattggcccc attgcctggc tgcccatgcc
     5941 ttgtttgatc atcgggaaga ggatctgctc ttctttcatc atgtggctgg aaagctcttc
     6001 atgcagcatg gtcaggtatt ttgtcagccc ttttggcacg ctcggtttgt cggcgtgaac
     6061 gcgctcgact ttagtcgctt gcagaatcag ctccggcagt tgctcgcggt gacgagcgtg
     6121 gtagcgcacg atgatatggt cgatgatttc tgccagcggg gcgctacgcc agtctttctc
     6181 aatcggttgt tcagcaagct ttgccagctc agcttcaatg acctcaacat ccagttcttt
     6241 acgtgccgcc gcacgcgcca gcgtttgctt accgccgcag cagtaatcca tatcatattt
     6301 acgaaacaga gccgaagcgc gaggaataga gagcgccagt tcacctaaag gttggtcgcg
     6361 ataagccata gctgatacct cattcttaat aagataagat gtattttaaa tgcatcttta
     6421 aggcaaaagc tataaccctt acgtagcaga ggcttattta actcactgcc agcgtgtcaa
     6481 ctttctgaga cagattcagt taaaggtttt tctggttttg ctctaaccgc gatgaccacg
     6541 ccaatcacta acagcgcgat accgctcagc gtcattagcg gcggcatttg ctggcggagt
     6601 aaaaaggtgt acagcaaacc tgccagcgtt tcgaaaacaa tcagcggccc gagaatcact
     6661 gtcggtaatc gctggctggc gacgttccag cagagtgcgc caacccatga gcaaagcacg
     6721 gctatcgcaa tcatcagact aataaacacc agcggacggg ggccaaaggg tagggagaag
     6781 tccggcgttt gcgtattcag ccagtaacaa gcgacgagat agccgataag tgaaaccggc
     6841 agtgtgacca gcgcctgcgc tgttgcccac atcatcggat gtttgtcggg attttcccgc
     6901 agccagcggg cgttgcgcag ggcataccac gcccagcaga ccacggaaac taacgccagc
     6961 acgatgccag aggtataacg tgcccagtca aaattgggga gtccgtggtt taactcagca
     7021 atattcacac acgccaggcc gatgccaata caaatcagtg ccggggcgag ttttccccac
     7081 gcgagtttgc cgtcgcgctg gctataaagc agattggcga agacgggaat caccaccggc
     7141 agggtgccga taatcatcgt ggaaacaggc gcaccagtac gttgaatggc actggcaagg
     7201 cagaagtaat agatgaggtt gcccatcata gtgagcatca aggcggtaag ccagtcgcga
     7261 cgcgccaact gacgcagacg cacgcgtccc agccaggcaa tgggcagcgc aattaaccct
     7321 aacgccagat aacgccccat cgactgcaac atcgccgggt attccggcac gatcaacggc
     7381 ccgacaaaaa taagccccca catcaaccct gctaacaggg cgtacagcac gccgctaatc
     7441 attactggca tccattgatc tgtcagaaga aagttcagga agcagacatg attgctcccg
     7501 gcatcccgtc attatcagag aggatgcggg aggatgccac tttaattaac tagttattaa
     7561 cattaaaaag atggcttacg ctttcggcta acgttgtcgt tgggtggcca atcaatttgc
     7621 taagtgtttt gctgtcatca aacagaccgc ctttcgatgc gccaacgtca gaatccgcca
     7681 gcatatccgc cagtccgtcg ggcagtccga cgcttttcag tgccgcggcg aaatcggctt
     7741 cgctcagatt ttgataggta acctgtttgc cgctctgttt ggtcagctct gccgctaact
     7801 gtgtcaacgt ccaggcacta tcgcccgcca gttcgtaaac cttgccttcg tgaccggctt
     7861 cgctaatcac gcgtgccgca gctgccgcat aatctgcccg cgttgctgag gcgattttgc
     7921 catcgcccgc cgcaccgata aatacgccgt gttccagtgc tgccggggcg ctggcgaggt
     7981 agttttcggt gtaccagccg ttgcgcagta gggtgtaaac gatgccagaa tcagccagca
     8041 ttttctccgt ctcgatgtgc tcatcggcga ggccgagcgg agatttatcc gcgtgaagca
     8101 ggctggtata agcgataaat ttcacgccag ccgtctttgc ggcattaata acattacgat
     8161 gctgcggggc acgttgaccc acttcgctgg aagagatcag cagtagtttt tccactccct
     8221 gaagtgcaga tgtcagtgcg gcttcatcgc cgtagtcagc ctgacgcacg gtaatgcctt
     8281 gtgctgtcag tgcctgggct tttgccggat tacgaacgat agccactatt tggctggcag
     8341 gaaccgtttt catcaaggat tcaataacat actgaccaag ttggccagtg gcaccagtaa
     8401 tagcgatcat gggaagtctc catcgttttg tcttgtgttt aaatacgcta acacctaaac
     8461 ttacttttag taagtacgca caaaaaggta agtatgaaat gagtcaggtt agcctgtcgc
     8521 aacaactgaa agagggtaat ctctttgcgg aacagtgccc gtcgcgcgag gtgttgaaac
     8581 acgtcaccag ccgttggggg gtgttgattc tggtggcgct acgcgaaggt actcatcgct
     8641 ttagcgacct gcggcgcaaa atcggcgggg tgagcgaaaa gatgcttgcg cagtcgttac
     8701 aggcgttaga acaggatggt tttcttaacc gtatcgcgta tccggtggtg ccgccgcatg
     8761 ttgaatacag cctcacgccg ctgggcgagc aggtgagcga aaaggttgct gcactggcgg
     8821 actggattga gttgaatttg cccgaggtgt tggcggtgcg ggatgaacgt gcggcataac
     8881 tgccggatgc gcggcctacg gtgtatgact tgtagggcgg ataagatgcg ccagcatcgc
     8941 atccgccaat aaatgccgga tgcgattgat gatttatagg ccgaaaaaga agtgttttac
     9001 ttactcaaat ccacctgata aatcgcaaac ccgatatcat cggtcgcgac tttattcatc
     9061 ggatactgcc ctttctcttt aataaacgct gcggctttat ctgacgggga agtttcgaaa
     9121 cggatatcca gtttcttatc ggcagctatc ggtgctaaac gccagttgtt atctgccgcc
     9181 gggtgaattt cccccgcacg tttcgactca tcagcaatcc acgctgccag caccgagcgg
     9241 ttctcatccg gtgaagcaaa agcgatatgg ctgtcgcccg tcccggcaaa tttgccgccg
     9301 taagcgcgat agttattggt ggcaacgagg aacatagcgt tcggatcaat cggcttgcca
     9361 ttaaaggtca ggttcttaat cctttccgca ttggcattaa tcatctggca ctcgccgtca
     9421 taacgggcgg gctgggtaac atcaatctga taattcacac catcaataac atcaaagtta
     9481 taagtgcgga aaccatccca gttgatgagt gactgtggtt tcgtgctgtt gggatcaatc
     9541 tggttaaact gtccggcgga gcactccagc cactctttca cctctttacc gctggctttc
     9601 accacaatca gcgtattggg atagagataa agatcggcgg cattacggaa ggtcaactgg
     9661 cctttttcca cctccacata gcttgccggg tcatttttgc gaccaccgac tttaaacggt
     9721 gcggcagctg aaagcaccgg cagttttgcc agatccggat cgccctgaat gtaatgctcg
     9781 acatacgctt tttgcgcgtt gttcactact tgcacggtcg gatcgtcctg caccagcgcc
     9841 agatagctat acatattgtc ggcagattta ccgattggct tgctgacgaa ctggcgtgtg
     9901 gcatcgtgat cggctttgag tgtttctacc agcttgctgt cttccgccgc gagggatttt
     9961 ttattggcga tgtcgtaaat cggccgtgct tccgctttcg cctgcgtcac ctgccattta
    10021 ccgctgtcat tactgagttg taagtcgacc accccaagat gatcgcccca catgcccggc
    10081 attaccgccg gaacaccatt cagcgtgcct ttggtgatat cagccccttc gatatcagca
    10141 aaatctttac ccgggaaaac ggcgtgagca tggccaaaca taatggcgtt aacgcccgga
    10201 atttcactga ggtaataaac tgagttttcc gccattactt tatacggatc ggcagacagc
    10261 ccggaatgcg ccagaacgac aacgacatcg gcacctttct cgcgcatttc aggctcgtat
    10321 ttgcgcacgg tttcggtaat atcattcacc gtcacttttc cggataaatt agctttatcc
    10381 cagcccatga tttgcggcgg cacgacgcca atatagccaa tcttcagcgt ctgttttttt
    10441 ccgtctttat cgaccacttc ggtgtcttta attaaatacg gtgtaaacat tggctgtttg
    10501 gttctggcgt caatgacgtt ggcatttaca taagggaatt tcgctcctgc cagcgcattt
    10561 ttcaggtaat ccagaccgta gttaaactcg tggttgccaa gcgttccgac ggtatagtcc
    10621 agcgtattta atgccttata gaccgggtga atatcacctg cttttaatcc tttcgccgac
    10681 atgtaatcgg ccagcggact cccctgaatc aaatcgccgt tatcgaccag tacgctgttt
    10741 ttcacttcat tgcgggcatt gttaatcagg cttgccgtac gtaccagtcc gaatttttcc
    10801 gtggcggtgt ctttgtaata atcgaaatcc atcatgttgc tatgcagatc agtggtttcc
    10861 atgatacgca gatcgaccgt cgctgcattc acactggcgg caatcagcgt ggccaggagc
    10921 gttgcgctaa acttaatcat cagggacatc cttttatcat cgggaatacg aaagaaaagg
    10981 gagaataaac gtcttactta tagaacagtg aagaatgcca caattttacg ctttgaaaat
    11041 gatgacacta tcacagttgg cgcattcatt aatgataggg tataagtaaa acaataagtt
    11101 aacaccgctc acagagacga ggtggagaaa tgttagatca agtatgccag cttgcacgga
    11161 atgcaggcga tgccattatg caggtctacg acggggcgaa accgatggac gtcgtcagca
    11221 aagcggacaa ttctccggta acggcagcgg atattgccgc tcacaccgtt atcatggacg
    11281 gtttacgtac gctgacaccg gatattccgg tcctttctga agaagatcct cccggttggg
    11341 aagtccgtca gcactggcag cgttactggc tggtagaccc gctggatggt acgaaagagt
    11401 ttattaaacg taatggcgaa ttcaccgtta acattgcgct cattgaccat ggcaaaccga
    11461 ttttaggtgt ggtgtatgcg ccggtaatga acgtaatgta cagcgcggca gaaggcaagg
    11521 cctggaaaga agagtgcggc gtgcgcaagc agattcaggt ccgcgatgcg cgcccgccgc
    11581 tggtggtgat cagccgctct catgcggatg cggagctgaa agagtatctg caacagcttg
    11641 gcgaacatca gaccacgtcc atcggctctt cgctgaagtt ctgcctggtg gcggaaggac
    11701 aggcgcagct gtacccgcgt ttcggaccaa cgaatatttg ggacaccgcc gctggacatg
    11761 ctgtggctgc agctgccgga gcgcacgttc acgactggca gggtaaaccg ctggattaca
    11821 ctccgcgcga gtcgttcctg aatccggggt tcagagtgtc tatttactaa attcagatgg
    11881 cagaaacagt gtatttcctg attctgccat cctgatttct cccaacctaa aaagttataa
    11941 ataaaaagag attgtattta aagtgcaaaa attcaattgc taataagtta cattttaata
    12001 atga
//
LOCUS       DBFIFM010000134        11997 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.134, whole genome shotgun sequence.
ACCESSION   DBFIFM010000134 DBFIFM010000000
VERSION     DBFIFM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.134"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            24..230
                     /locus_tag="N7C74_003266"
     CDS             24..230
                     /locus_tag="N7C74_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433082.1"
                     /translation="MAEEKKSGVSVYISPEIVEVLKQRHKKNYEAGVAAGLDPLMTPE
                     PSIGSLVRSYLLAALGMHKNYGGE"
     gene            230..1285
                     /locus_tag="N7C74_003267"
     CDS             230..1285
                     /locus_tag="N7C74_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU2433083.1"
                     /translation="MAGKATALNTNQLFMYLNRGDIADFKFSPLFTTLFFPNVATFST
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPENCIIEQAKGKKWSEQDKDMHDPIYDIELYADQAGCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAELACKNLGEVVSFKGYLGDLALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMFYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            1298..1633
                     /locus_tag="N7C74_003268"
     CDS             1298..1633
                     /locus_tag="N7C74_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGU2433084.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLADNGEFK
                     PWDGQESGKAVYLTSYPVDTSKQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            1646..2059
                     /locus_tag="N7C74_003269"
     CDS             1646..2059
                     /locus_tag="N7C74_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HGU2433085.1"
                     /translation="MATNESIMTLPLASKFKVEARAMADRGLSTYEAVYQLNKLEEQD
                     KPCADAIMALHEHKDYQPLLRAMANVPCIDVGTAKSILSMTIEQERPKVAPELTAAFE
                     NFMDMHSPQAVSAGMAYDGRNQGDDGDIDRILKTI"
     gene            2265..2807
                     /locus_tag="N7C74_003270"
     CDS             2265..2807
                     /locus_tag="N7C74_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU2433086.1"
                     /translation="MKSHLMNKKNMAKSCRVSATAFDKWGVTPVERKGREAFYDVASV
                     IENRVNNAISQLINDKGEIDDDELLRVRIRLLTAQAEAQELKNDRDRGDVIDTEFCLY
                     ALSKLASQISSIMDSLPLTMQRSFPQITPAMLDRLKREVVKACNASARVADNLPQILA
                     DYLKETTGNVPEKLLQKKGE"
     gene            3063..3344
                     /locus_tag="N7C74_003271"
     CDS             3063..3344
                     /locus_tag="N7C74_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433087.1"
                     /translation="MTEAELLGLIRRVAGISQQHDEQATQPDSVTAENYVRVVAEVMR
                     RDGIELNGVDMRNIRNRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(3945..5405)
                     /gene="phoQ"
                     /locus_tag="N7C74_003272"
     CDS             complement(3945..5405)
                     /gene="phoQ"
                     /locus_tag="N7C74_003272"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HGU2433088.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMHGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITKQYEGKIVAGESMLGGARMEVIFGRQHSTPKDE"
     gene            complement(5405..6076)
                     /gene="phoP"
                     /locus_tag="N7C74_003273"
     CDS             complement(5405..6076)
                     /gene="phoP"
                     /locus_tag="N7C74_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HGU2433089.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLICRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(6245..7615)
                     /gene="purB"
                     /locus_tag="N7C74_003274"
     CDS             complement(6245..7615)
                     /gene="purB"
                     /locus_tag="N7C74_003274"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HGU2433090.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(7619..8260)
                     /gene="hflD"
                     /locus_tag="N7C74_003275"
     CDS             complement(7619..8260)
                     /gene="hflD"
                     /locus_tag="N7C74_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HGU2433091.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLKRKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(8296..9402)
                     /gene="mnmA"
                     /locus_tag="N7C74_003276"
                     /gene_synonym="trmU"
     CDS             complement(8296..9402)
                     /gene="mnmA"
                     /locus_tag="N7C74_003276"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HGU2433092.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPI"
     gene            complement(9456..9917)
                     /gene="nudJ"
                     /locus_tag="N7C74_003277"
     CDS             complement(9456..9917)
                     /gene="nudJ"
                     /locus_tag="N7C74_003277"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HGU2433093.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(9927..10580)
                     /gene="rluE"
                     /locus_tag="N7C74_003278"
     CDS             complement(9927..10580)
                     /gene="rluE"
                     /locus_tag="N7C74_003278"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HGU2433094.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATE"
     gene            10752..>11997
                     /gene="icd"
                     /locus_tag="N7C74_003279"
     CDS             10752..>11997
                     /gene="icd"
                     /locus_tag="N7C74_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HGU2433095.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYELAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKN"
BASE COUNT         3072 a         2952 c         3079 g         2894 t
ORIGIN      
        1 tagtcgaaga ggaatttctt actatggctg aagagaaaaa aagcggtgtt tcggtgtaca
       61 taagccccga aatcgtggag gtgctcaagc agcgccacaa aaaaaactat gaagctggcg
      121 ttgcggctgg actggatccg ctgatgacgc cggagccgtc gataggttca cttgtacgct
      181 cttatttact tgcggcgctt gggatgcata aaaattatgg gggtgaataa tggcaggcaa
      241 agcaacggca cttaacacta accagctttt catgtacctg aatcgcgggg atattgcgga
      301 ttttaaattc agccctctgt ttaccacgct gtttttcccg aacgtggcga cattcagcac
      361 gcaaaacatc atgctggata ccctggacat tgaagaagtc accatgtcgg cgttttgttc
      421 gcctatggtg ggtagccagg ttcagcgcga taaagggtac gaaaccagca caatcaaacc
      481 tggctacatg aagccaaagc acgaaatcga tccaacgaaa accatcatgc gcatggctgg
      541 agaagatccg gcacagctta acgaccctac ctatcgccgt atgcgcctga ttactggcaa
      601 catgcgccgc cagataaacg ccattaaggc acgcgtggaa tggctggcgg tgaatgcggt
      661 aacgaccgga aaaaacatca ttgagggcga aggcatagaa cgctatgaaa tcgactggaa
      721 aataccggaa aactgcatca tagagcaggc caagggtaaa aaatggtccg agcaggataa
      781 agacatgcac gacccaatct atgacatcga gctttatgct gatcaggctg gttgccccgc
      841 aaacgtcatg attatgggcg ctgaggtatg gcgcacatta cgcagcttta aaaaattccg
      901 tgagctgtac gatctttccc gtggttcaga atccgccgcc gaactggcct gtaaaaacct
      961 gggcgaagtg gtgagcttta aaggctatct tggtgatctg gcccttatcg tctattccgg
     1021 caaatacact gacagcgatg gtaccgaaaa atatttcctt gagcctgatt tgctggtcct
     1081 gggtaacacc aacaataaag ggctggtggc ctatggtgcg ataatggatc aggaagcggt
     1141 aagaacgggc gcaacacaaa acatgtttta cccgaaaaac tggattgagg acggcgatcc
     1201 ggcgattgag tacgtgcaga cacacagtgc accgcagccg gtaccggcag atattcgcaa
     1261 atttgttacc gtcaaaattg gttaacgggg ggattctatg gacactccat acattgagtt
     1321 atttgcaggc agtcagcagg tatccacgac gttggtacat tttgccgctg atgctggcgt
     1381 tattcaggaa tttaccccgc tgatgctggc agacaatggc gagtttaagc cgtgggatgg
     1441 tcaggaatct ggcaaggctg tttatctgac ctcgtaccct gtggacacgt caaagcagaa
     1501 atcagcacag tgttacaaga cggggatatt caatattgcc gccgttaact ggcctgagag
     1561 cgtcgacacc gatgcgaaaa aatgcgccgc ctttgcgggt tccggcgtat ccgttcagcc
     1621 gctggcccga taagcagggg gaacgatggc aacgaatgaa agcatcatga cgctaccgct
     1681 ggcgagtaaa tttaaagtcg aagcgcgggc aatggctgac agaggtttat caacctatga
     1741 ggccgtatat caactaaaca aactggaaga gcaggacaag ccgtgcgctg atgcgattat
     1801 ggcgcttcat gagcataaag actatcagcc gctgttgcgt gcaatggcaa acgtgccttg
     1861 tatcgatgtt ggtacggcta aaagcatcct tagcatgacc atagagcagg aacgtccgaa
     1921 ggttgcgccg gaacttaccg cagcctttga aaactttatg gacatgcaca gcccgcaagc
     1981 cgtatcagct ggcatggcat acgatggcag aaaccagggc gatgacggcg acatcgatcg
     2041 catactgaaa accatctgag acaaggccgg agaaatccgg cttttttacg ggtccttcct
     2101 ggaattatgg cccgttacgg ggcggcgacc tcgcggattt tcgctattta tgagcctttt
     2161 cagggtggtg gtggtggttt tgttgtttga tctatcttta tgaatgaaaa gggaaagatg
     2221 caagcaatac accaacctga agcagtaatt aagttggtgt attaatgaaa tctcatctga
     2281 tgaacaaaaa aaacatggcg aaaagctgcc gtgtaagtgc gacagcgttc gacaagtggg
     2341 gagtaactcc cgttgagcgt aaaggccgtg aggcgtttta tgatgttgcc agcgtgatag
     2401 aaaatagggt taacaatgca attagccagc ttataaacga caaaggcgag attgacgatg
     2461 atgaactttt acgagtcagg atcagattac tgacagcaca ggcagaggcg caggaactta
     2521 aaaacgatcg cgatcgcggt gacgtgattg atactgagtt ttgcctgtac gcgctttcaa
     2581 agctggcgag tcagatttca tcaatcatgg acagcctccc gcttactatg caaaggagct
     2641 tcccacagat tacccccgcc atgctggaca ggcttaaaag ggaagtggtt aaagcctgta
     2701 atgccagtgc cagagttgcc gacaacctcc cacagatact ggctgattac ttgaaagaaa
     2761 caaccggaaa cgtaccggaa aagttgctac agaagaaagg cgagtaacag acgcgcaatt
     2821 attgaacaaa aatgagaaag gatctgataa tggcattttc tggctaaaat gcctcgtgtc
     2881 agatcctttc tttttttaat attttcattg ttaaacagtc tgttacagag aagaagatcg
     2941 gatctgactt ttgccggaaa attctcataa acagataaaa accgcgaggt cgccgccccg
     3001 taacgggcca taattccagg aaggacccga cgacaccaga ctatcagagc gatgggggca
     3061 caatgacaga agccgaacta ctgggattaa tccgccgcgt cgccggaatc agccagcagc
     3121 atgacgaaca ggccacacag ccggacagcg tgaccgctga aaattatgtg cgtgttgtag
     3181 cggaagtgat gcgccgtgat ggtatagaac ttaatggcgt tgatatgcgc aacatacgga
     3241 accgcgttct tgaaatgctg gcctacaatc gccgcgttga actgtatcgg gagaaagaaa
     3301 aaataacgta ccactggaag aagccggaac ggctgcggcg gtaacttgct gatattcccg
     3361 ataacgcaaa attgcgctgg ctacatcctc acatctgagg actgttaatc agaaggccgg
     3421 aatatattca caaactacga aattttcgta gtttaattaa tggtcagatt aatcgctaac
     3481 ccgctgatat tttcggaaac ctcaatttga ggaagtcgga agcgtcaccc ttcatattct
     3541 gaatggtcat aatgaccacg cagaccatca aggcaaccac aacgaacatc gttacggttg
     3601 gagcttttac cccactgggg gaaaggtatt acgataatca taacacctac cgaacaggta
     3661 aaacccacca gcctgattaa cagttaaccg gaaaaaaaat ccggtagccc cgttatgggg
     3721 atccccatat cgacattaac gcccctcatg aattggtgcg cttccccctg gaaagattac
     3781 ccgcctgtat atttcttgtg tctatttgtt ccacgttgtt tcatacagtg caccgaacgg
     3841 tgtagttaat ggtgtagtca atttgctgtt tttagcactt tttgcagtgt gggttgttta
     3901 attaaatcaa tgggttgcct tgctgtggtg ataaaaggac atatttattc atctttcggc
     3961 gtagaatgct ggcgaccaaa aatcacctcc atccgcgcac cgccaagcat gctctctccg
     4021 gcgacgattt taccctcata ttgcttggtg atttcgcggg ctaccgccag ccccacacct
     4081 tgcccagggc gtaaagtatc aacccgttga ccacggtcga aaatgacctc tcgcttgctt
     4141 aatggaatac cggggccatc atcctcgacc acaatataga gatgctcgtc ggtttgcctt
     4201 gcagaaattt cgacaaactc gaggcaatat ttacaggcat tatccagtac attgcccatc
     4261 acctcgacaa aatcgttctg ctcaccgaca aagctgatct ctggcgaaat atcgagagag
     4321 atattgaccc ctttgcgttg atacactttg ttcagcgccg aggtgagatt gtccagcagt
     4381 ggggcgaccg gatgcagctc gcggctgagc aatgtcccgc cgtgcatact ggcacgatgc
     4441 aggtagtagc caatttgctg tgaaatgcgg ctgatttgct ccagcattac cggttcagca
     4501 tcactgacgc tcatcttttc actacgtaga gaacgcagcg tactttgcag caccgccagt
     4561 ggcgttttca gactatgggt caggtcggtg agcgttgtac gatatttgtc gtaacgttcg
     4621 cgttcacttt ttaacaatcg gttcaggttt cgtaccagac tggtcagttc tcgcgttgtg
     4681 gctggattga gcaattcgcg gttatgttct tccagttcgc ggacttcttt tgccagggct
     4741 tcgatggggc gtaaactcca ccaggcggcg acccacagca gcgggatcac taacagcaga
     4801 ttggctgaga gcacatagat aaaccagctc cagaccatat aggaactttt tagctccacc
     4861 ggaatggtat ccaccaccac aatggttaac tttggcatcc gcgatgttgc cgggtagacg
     4921 tttaccgcca ccgagtgggt catctccgcg tcgtcatcat cttcccgcac ttcctgcaac
     4981 tgttgctgta tcgaatgatc tccactcagc aagaggctgg tatcgttaac atccgcttca
     5041 atttcatgaa aaccattcga tttcagccag tcaggctgga tcatcttcat cagccagggc
     5101 acgtcacgtt gcgcccataa aagctgcccg ttctcatcat aaattagcgt catggtgggg
     5161 ctttgcttgt cgatattttc gggtaactcg acatgcaact tattgttttc ccacttcgca
     5221 agggtataga acagattgct ctcgccacgt aacagccgaa acgtagtttt atcgaaactg
     5281 acgctataac cgatcagcgc gaccattccg taggcaagcg aaagcaccag tactaccgct
     5341 gccgttgcca acagaaaacg tacccgcagc gagagcggga aaaaaagacg cagtaatttt
     5401 ttcatcagcg caattcgaac aaatagccct ggccgcgaac ggtagtaatc acttcttgcg
     5461 gatattgtgc ctgaattttt ttgcgcagac gtcccatcag tacatcaatg gtatggcttt
     5521 cccgcagctc cgcatccgga tagagttgta gcattaacga atctttgctg accactttgc
     5581 cattattgcg tatcaacgtt tccataatgg tgtattcgaa cgcggtcagt ttgatcactt
     5641 cgtcattaat agataattca cggcgagaga gatcaacctg aaacgggggg agcgaaatga
     5701 cctgtgaagc caggccgcta ttacgccgca ttaatgcctg cattcgcgcc atcacctctt
     5761 caatatgaaa cggtttagtc acgtaatcat cagcaccggc acttaatact tcgactttgt
     5821 cctgccagct ttcacgggcg gttaatacca gaatcggcag tgaaacatca ttgctacgcc
     5881 agcggcaaat cagtgacaga ccgtcctcgt ctggcaatcc gagatcgaca atcgcaatat
     5941 ccggtaaatg ttcattgaga taataatcgg cttctttggc atcttctgca tcatcgacct
     6001 gatgaccagc atcctgaatc tgaactttaa ggtggtgacg taacaacgca ttgtcttcaa
     6061 caaccagtac gcgcattttt atttctccct gtcttaatta ttaaaatagt gtaacgcgat
     6121 tatgtggtta tgggggtaaa cattaaataa accagcgggg gaggggaggt aaagtgaaaa
     6181 aataaaaagc ggataatctt aataagcagg ccggacagca tcgccatccg gcactggtac
     6241 gagtttattt cagctcatca accatggtga tggcgcgacc aatgtagtta gccggcgtca
     6301 tcgctttcag gcgggctttc tcttcttctg gcaacgccag accgtcgata aactgcttca
     6361 tgccttcggc gtcaacgcgc ttaccgcgag tcagctcttt cagcttctcg tacggttttt
     6421 cgatgccata gcgacgcata actgtctgga ttggttctgc cagcacttcc cagttgtgat
     6481 ccagctcatc cagcagatgg tcacggttca cttccagttt gctcacgcct ttcagggtgg
     6541 attgatacgc aatcagcgca taaccgatac ccacgccgag gttacgcagc acggtggagt
     6601 cggtcaggtc acgctgccag cgggaaaccg gcagtttgct tgccagatgc tgcaataccg
     6661 cgttggaaag gcccagattc ccttcggagt tttcgaagtc gatcgggtta actttatgcg
     6721 gcatggtgga agaaccaatc tcaccagcaa tggttttctg tttgaagtgg ttaagggcga
     6781 tataacccca gacgtcacgg tcaaagtcga tcagaatggt gttgaagcgc gcaacgcaat
     6841 caaacagttc ggcaatgtag tcgtgcggtt caatctgggt agtgtacggg ttccattgaa
     6901 tacccagcga ggtgacgaac tcttcgctga actgatgcca gtcaacttcc gggtaagcgg
     6961 cgatgtgggc gttatagtta ccgaccgcgc cgttgatttt gccgaggatc tccacctggt
     7021 taagctggcg gtactggcgc tccatacggt aggcgacgtt tgccatctct ttaccgatgg
     7081 ttgacggcgt ggctggctga ccgtgggtac gggacagcag cgggatatcg cgatactgaa
     7141 cggcgagatc tttaatgcca tcaattagtt gacgccagta cggcaggatc acttcatcac
     7201 gcgcggtttt cagcattaat gcgtgggaga ggttattgat atcttccgaa gtacaggcaa
     7261 agtggatgaa ttcagaaacc gcgtgcagtt ccgggatctc cgccactttt tctttcagga
     7321 aatactcaac cgctttaacg tcgtggttag tagtacgctc gatggttttg atgcgtgcgg
     7381 catcttcttc gctgaaattg gcgacaattg catcaaggta accgattgcg tcggcagcaa
     7441 aagcaggaac ttccttgatc gctgcgtgcg cggccagttt ttgcagccaa cgtacttcaa
     7501 cttgtacacg gaatttcagc aaaccatatt cgctgaaaat cccgcgcagc gcgctgactt
     7561 tatcgccgta gcgtccatcg acaggggaaa cggcggtcag tgaggataat tccatagatc
     7621 acaactccgg ggttaaatga gcaagaattt gttttgcctg agtggtcagg cgattacgag
     7681 aaaacatcag ttgcagacgt ccgccgccga cctggtgcca gagcacggcg gcgcgaatgc
     7741 ctgccagcag ggttgcgcga actttcgcct gcacttgtgg gctttgcagt acagcagggg
     7801 aaccggtgac ctgaatgcgc gggccaagcg ggctgatcac atcaacatag atagcagcca
     7861 tcgcgctcat cagcgtttcg gactgtaaat cgaagtgttc gagctggcgt tgcaggccgt
     7921 tgatccggtt gcccagagtg tcgagcgcac ctttcgctga ggagagtttg cgcttaagca
     7981 ccatcaggct gagtgtgtag cgggttaatt cggcgtttaa gccctggcga ctgctggcat
     8041 tgagcacgcc gagcagggtt tccagcccga cgcgcaggtt ggcttcgcta ccgccaaaaa
     8101 ccgccagcgt cgagctgggg ttcatatcaa taatactgtt gagtgagacg tgtagcgcat
     8161 cggcatcaca atgcccctgg tgagcgagtt gttgcaccag gcgtgccgac tgacaaatac
     8221 cggccagggc gagggtgatg tcatagtaat tctttgccac gttcactgct tccttgtttt
     8281 aagtaaagat aataatcaga tcggcagcgg cagacgctgc tcaataatac cgccaccgag
     8341 gcacacttca ccgttataga agacggcaga ctggcccggc gtcacggcgg caacgggctc
     8401 atcgaaaatc acttcaatgc gatcatcgtc cagcgccttg acggtgcagg ggatatcggt
     8461 ctggcgatag cgggttttta ccgtgcaacg catagtaccg gtgaatggtt cacgatcgac
     8521 ccagtgcagc tgctgggcaa tcagaccgac agacatcaga cgcgggtgtt catggccctg
     8581 cgcaacaatc agaatgttgt tttcgacgtc tttatcgacc acataccacg gttcttcggt
     8641 gccttctttg gtgccaccga tacccagacc tttgcgctga ccgagggtgt gatacatcag
     8701 cccctgatgc tcaccaattt catcgccatc gactgtaatg attttgcccg gttgcgccgg
     8761 gagataacgg ccaaggaact cgcggaattt acgttcgcca atgaagcaaa tgccggtaga
     8821 gtcttttttc ttcgcggtga ccagaccaag atcttcagca atcttacgca cctgcggttt
     8881 ttccagttcg ccgaccggga acaggctttg cgcaatctgc tcatggctga gcgtataaag
     8941 gaagtagctc tggtctttat tgctgtccag accacgcagc aggcggctct tgccatcgac
     9001 atctgcccga cgaacgtaat gaccggtagc gatataatcg gcacctaaat cttcggcggc
     9061 aaattcgagg aaggctttaa atttgatctc tttgttgcac agaatatccg gattcggcgt
     9121 gcgaccggct ttatattcgg caaggaacag ttcgaagacg ttgtcccagt actcggcagc
     9181 aaagttaacg gtgtgcagtt caatgccgag cttgtcgcag acagcctggg catcagccag
     9241 atccgctgcc gctgtgcaat attcctcacc gtcgtcttct tcccagttct tcataaacag
     9301 gccttcgacc tgatatccct gttgttgcaa cagccaggca gaaacggagg aatcgacacc
     9361 gccggacatg ccgacgatta ctttttttgc ggtttcagac attggatcac tcacgacatt
     9421 gaacttcaag gcggcgtatt ctatcacgca gccttttaga tgacaccctt tgtaaaaggc
     9481 cagttaaaat cgccaatcat ctccagcgga tagcgttgcc cgctttgata acaacggata
     9541 ctttccgcca ccagcggcga acgaagattt gacgcctgta aaatttcttc ggcgctgacc
     9601 caacgacagc agtcgatatc gctgtcatga ggctgggtcg ggcatatttg ctcaagctca
     9661 atggcaaaga ggaaacgcaa aaacggcgtt ttatctggcg caatccactg atgcatacga
     9721 ataaagtgtt gcggctgcgc gctgatgccg gtttcttccc acagctcacg ggcggctgct
     9781 tccactaagg tttcatcggc ttccagatgc ccggcaggtt ggttccataa ggctttacca
     9841 ttaatcgtct cttcaacgac taaaaatttc ccctctgcgt gcactacgca agcaacggta
     9901 acgtgcggtt taaacattgt gcatccttat tctgttgctt ctcgccattc gccattggca
     9961 aggttatcca aagagtaatc acccatcgca tagcgaatca gtcgcagcgt ggggaaacca
    10021 acatgggcgg tcatgcggcg cacctggcga ttacgtcctt catataaggt gatcttcagc
    10081 cagctggtgg gaatactttt gcgttcacga attggtggat tccgcggcca taaccacgcg
    10141 ggttcgtcaa ccagctccgc gcctgcgggc agggtagggc catcatttaa ggttacgcca
    10201 ttgcgcaagg cttcaagtgc gtcttgtgtg ggaatacctt ccacctgcac ataatagatt
    10261 tttccggtgc gtttgcccgg ctgggttaaa cgcgcctgca acgcgccgtt attggtcagc
    10321 accagtaacc cttcgctatc gcggtcaaga cgacctgctg cataaacgcc ctgaaccggg
    10381 atgaattctt ttaatgtttt gcgtccggct tcatcggtga actgcggaag aacatcgtag
    10441 ggtttattga acaggatcac acgcgtgggc tggttttcag gtttacgcct ggtagaacgt
    10501 tgtgagctga atcgcttaac ctggtgattt ctaaaagaag ttttttgcat ggtattttca
    10561 gagattatga attgccgcat tatagcctaa taacgcgcat ctttcatgac ggcaaacaat
    10621 agggtagtat tgacaagcca attacaaatc attaacaaaa aattgctcta aagcatccgt
    10681 atcgcaggac gcaaacgcat atgcaacgtg gtggcagacg agcaaaccag tagcgctcga
    10741 aggagaggtg aatggaaagt aaagtagttg ttccggcaca aggcaagaag atcaccctgc
    10801 aaaacggcaa actcaacgtt cctgaaaatc cgattatccc ttacattgaa ggtgatggaa
    10861 tcggtgtaga tgtaacccca gccatgctga aagtggtcga cgctgcagtc gagaaagcct
    10921 ataaaggcga gcgtaaaatc tcctggatgg aaatttacac cggtgaaaaa tccacacagg
    10981 tttatggtca ggacgtctgg ctgcctgctg aaacccttga tctgattcgt gaatatcgcg
    11041 ttgccattaa aggcccgctg accactccgg ttggtggcgg tattcgctct ctgaacgttg
    11101 ccctgcgcca ggaactggat ctctacatct gcctgcgtcc ggtacgttac tatcagggca
    11161 ctccaagccc ggttaaacac cctgaactga ccgatatggt tatcttccgt gaaaactcgg
    11221 aagacattta tgcgggtatc gaatggaaag ctgactctgc cgacgccgag aaagtgatta
    11281 aattcctgcg tgaagagatg ggcgtgaaga aaattcgctt cccggaacat tgcggtatcg
    11341 gtattaagcc gtgttctgaa gaaggcacca aacgtctggt tcgtgcagcg atcgaatacg
    11401 caattgctaa cgatcgtgac tctgtgactc tggtgcacaa aggcaacatc atgaagttca
    11461 ccgaaggcgc gtttaaagac tggggctacg agctggcgcg tgaagagttt ggcggtgaac
    11521 tgatcgacgg cggcccgtgg ctgaaagtta aaaacccgaa caccggcaaa gagatcgtca
    11581 ttaaagacgt gattgctgat gcattcctgc aacagatcct gctgcgtccg gctgaatatg
    11641 atgttatcgc ctgtatgaac ctgaacggtg actacatttc tgacgccctg gcagcgcagg
    11701 ttggcggtat cggtatcgcc cctggcgcaa acatcggtga cgaatgcgcc ctgtttgaag
    11761 ccacccacgg tactgcgccg aaatatgccg gtcaggacaa agttaaccca ggttctatta
    11821 ttctctctgc tgagatgatg ttacgccata tgggttggac tgaagcggct gacctgattg
    11881 ttaaaggtat ggaaggcgca atcaatgcca agaccgtaac ctatgacttc gaacgtctga
    11941 tggaaggcgc taaactgctg aaatgttcag agtttggtga cgcgatcatc aagaata
//
LOCUS       DBFIFM010000135        11964 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.135, whole genome shotgun sequence.
ACCESSION   DBFIFM010000135 DBFIFM010000000
VERSION     DBFIFM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.135"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(53..961)
                     /gene="melR"
                     /locus_tag="N7C74_003280"
     CDS             complement(53..961)
                     /gene="melR"
                     /locus_tag="N7C74_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HGU2433096.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACIPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            1244..2599
                     /gene="melA"
                     /locus_tag="N7C74_003281"
     CDS             1244..2599
                     /gene="melA"
                     /locus_tag="N7C74_003281"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HGU2433097.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYAPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYDAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2702..4123
                     /gene="melB"
                     /locus_tag="N7C74_003282"
     CDS             2702..4123
                     /gene="melB"
                     /locus_tag="N7C74_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HGU2433098.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIAFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKIPPEPVHADIPVGAVSDVKA"
     gene            complement(4262..4891)
                     /locus_tag="N7C74_003283"
     CDS             complement(4262..4891)
                     /locus_tag="N7C74_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HGU2433099.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(5014..6660)
                     /gene="fumB"
                     /locus_tag="N7C74_003284"
     CDS             complement(5014..6660)
                     /gene="fumB"
                     /locus_tag="N7C74_003284"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGU2433100.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSVETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHHPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIINKQCANCTK"
     gene            complement(6738..8078)
                     /gene="dcuB"
                     /locus_tag="N7C74_003285"
     CDS             complement(6738..8078)
                     /gene="dcuB"
                     /locus_tag="N7C74_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HGU2433101.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(8649..9368)
                     /gene="dcuR"
                     /locus_tag="N7C74_003286"
     CDS             complement(8649..9368)
                     /gene="dcuR"
                     /locus_tag="N7C74_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HGU2433102.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(9365..10996)
                     /locus_tag="N7C74_003287"
     CDS             complement(9365..10996)
                     /locus_tag="N7C74_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGU2433103.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            11177..11407
                     /gene="yjdI"
                     /locus_tag="N7C74_003288"
     CDS             11177..11407
                     /gene="yjdI"
                     /locus_tag="N7C74_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HGU2433104.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            11419..11691
                     /locus_tag="N7C74_003289"
     CDS             11419..11691
                     /locus_tag="N7C74_003289"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU2433105.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
BASE COUNT         2898 a         2853 c         3097 g         3116 t
ORIGIN      
        1 ctgcttatta tcgacggcta aactattttt ttggctgata ctgatatcgt ctttagccgg
       61 gaaacgtctg gcggcgctgt tggctaagtt tgcggtattg ttgcggcgac atgccgacat
      121 atttgccgaa cgtgctgtaa aaacgactac ttgaacgaaa gcctgccgtc agggcaatat
      181 cgagaatact tttatcggta tcgctcagta acgcgcgaac gtggttgatg cgcatcgcgg
      241 taatgtactg tttcatcgtc aattgcatga cccgctggaa tatccccatt gcatagttgg
      301 cgttaagttt gacgtgctca gccacatcgt tgatggtcag cgcctggtca tagttttcgg
      361 caataaagcc cagcatctgg ctaacataaa attgcgcatg gcgcgagacg ctgtttttgt
      421 gtgtgcgcga ggttttattg accagaatcg gttcccagcc agagaggcta aatcgcttga
      481 gcatcaggcc aatttcatca atggcgagct ggcgaatttg ctcattcgga ctgtttaatt
      541 cctgctgcca gcggcgcact tcaaacgggc taagttgctg tgtagcaagt gatttgatca
      601 ccatgccgtg agtgacgtgg ttaatcaggt ctttatccag cggccaggag agaaacagat
      661 gcatcggcag attaaaaatc gccatgctct gacaggttcc ggtatctgtt agttggtgcg
      721 gtatacaggc ccagaacagc gtgatatgtc cctgattgat attcactttt tcattgttga
      781 tcaggtattc cacatcgcca tcgaaaggca cattcacttc gacctgacca tgccagtggc
      841 tggtgggcat gatatgcggt gcgcgaaact caatctccat ccgctggtat tccgaataca
      901 gcgacagcgg gctgcgggtc tgtttttcgt cgctgctgca cataaacgta tctgtattca
      961 tggatggctc tctttcctgg aatatcagaa ttatggcagg agtgagggag gatgactgcg
     1021 agtgggagca cggttttcac cctcttccca gaggggcgag gggagtgtcc gagcatcatg
     1081 aggccgaaaa ctctgctttt caggtaaatt attcccataa actcagattt actgctgctt
     1141 cacgcaggat ctgagtttat gggaatgctc aacctggaag ccggaggttt tctgcagatt
     1201 cgcctgccat gatgaagtta ttcaagcaag ccaggagatc tgcatgatgt ctgcacccaa
     1261 aattacattt atcggcgctg gttcgacgat tttcgttaaa aatattcttg gtgatgtgtt
     1321 ccatcgcgag gcgctgaaaa cggcgcatat tgccctgatg gacattgacc ccacccgcct
     1381 ggaagagtcg catattgtgg tgcgtaagct gatggattca gcaggggcca gcggcaaaat
     1441 cacctgccac acccaacaga aagaagcctt acaggatgcc gattttgtcg tggtggcatt
     1501 tcagattggc ggttatgcac cttgcacggt gactgatttc gaggtctgta agcggcatgg
     1561 tctggaacaa accattgccg atacgttggg gccgggcggt attatgcgcg cgctacgtac
     1621 cattccgcat ctgtggcaaa tttgcgagga catgacggaa gtctgccccg atgccaccat
     1681 gctcaactat gttaacccaa tggcgatgaa tacctgggcg atgtatgccc gctatccgca
     1741 tatcaaacag gtcgggctgt gccattcggt gcagggaacg gcggaagagc tggcgcgtga
     1801 cctcaatatc gacccggcta cgctgcgtta ccgttgcgca ggtatcaacc atatggcgtt
     1861 ttacctggag ctggagcgca aaaccgccga cggcagttac gtgaatctct acccggaact
     1921 gctggcggct tatgacgcag ggcaggcacc gaagccgaat attcacggca atactcgctg
     1981 ccagaatatt gtgcgctatg aaatgttcaa aaagctgggc tatttcgtca cggaatcgtc
     2041 agaacatttt gctgagtaca caccgtggtt tattaagcca ggtcgtgagg atttgattga
     2101 gcgttataaa gtaccgctgg atgagtaccc gaaacgctgc gtcgagcagc tggcgaactg
     2161 gcataaagag ctggaggagt ataaaaacgc ctcccggatt gatattaaac cgtcacggga
     2221 atatgccagc acaatcatga acgctatctg gacgggcgag ccgagtgtga tttacggcaa
     2281 cgtccgtaac gatggtttga ttgataacct gccacaagga tgttgcgtgg aagtggcctg
     2341 tctggttgat gctaatggca ttcagccgac caaagtcggt acgctacctt cgcatctggc
     2401 cgccctgatg caaaccaaca tcaacgtaca gacgctgctg accgaagcta ttcttacgga
     2461 aaatcgcgac cgtgtttacc acgctgcgat gatggacccg catactgccg ccgtgctggg
     2521 cattgacgaa atatatgctc ttgttgacga cctgattgcc gcccacggcg actggctgcc
     2581 aggctggttg caccgttaaa acgcgactaa acgctactgc gccgggggat ttattccggc
     2641 gcacacctct gacgatacca ataacagaag gcgggcgttg gtaacagcga ctcgataccc
     2701 tatgagcatt tcaatgacta caaaactcag ttatggattt ggagcgttcg ggaaggattt
     2761 tgcgatcggc attgtgtata tgtacctcat gtattactac accgatgtcg tcgggctgtc
     2821 tgtgggtttg gtcggtactt tgtttctggt ggcgaggatc tgggatgcta ttaacgatcc
     2881 gattatggga tggattgtaa atgctacgcg atcgcgatgg ggtaagttca aaccctggat
     2941 cctgatcggt acgttggcaa actctgtaat cttatttctc ctctttagtg cgcatctgtt
     3001 tgaaggtact actcagattg cctttgtttg cgtgacctac atcctctggg gcatgactta
     3061 caccattatg gatattccct tctggtcgct ggttccaacc atcacgctcg ataaacgtga
     3121 gcgtgaacaa ctggttcctt atccgcgttt ttttgccagt ctggcgggct ttgttacggc
     3181 aggtgtgacg ctaccatttg ttaattatgt cggcggtggc gatcggggat ttggctttca
     3241 gatgttcaca ctggtactga tcgccttttt tattgtttca accatcatca ctctgcgcaa
     3301 tgtgcatgaa gtcttttcgt cagacaatca accgtctgct gaaggaagcc atctgacact
     3361 taaagccatc gttgcgctta tttataaaaa cgatcagctt tcatgcctct tgggtatggc
     3421 tcttgcttat aatgtagcca gcaacattat taccggcttt gctatctatt atttctcata
     3481 tgttatcggt gatgcggatt tgttccccta ttatctgtcg tatgcgggag ctgctaacct
     3541 ggtgacgtta gtattcttcc cacgcttagt taaatcatta tcccgacgca ttttatgggc
     3601 cggagcatct attcttccgg tgttaagctg tggtgttctc ctgttaatgg cattaatgag
     3661 ctatcacaac gtcgtcctca ttgtgattgc gggtattttg ctgaatgtgg gaacggcgct
     3721 tttctgggta ttacaggtca tcatggtggc agataccgtt gattacggtg aatataaact
     3781 gcacgtacgc tgtgaaagca tcgcttactc cgtgcagact atggtggtga agggcggttc
     3841 agcctttgcg gcttttttca ttgcggttgt gttagggatg attggctatg taccgaatgt
     3901 tgaacagtct acgcaagccc tattaggtat gcagtttatt atgattgctc taccgactct
     3961 gtttttcatg gtaacgctga ttctctactt ccgtttctat cgcctcaatg gcgacacgct
     4021 gcgcaggatc cagattcatc tgctggataa atatcgcaaa ataccgcccg agcctgttca
     4081 tgctgatatt ccggtcggtg cagtgagtga tgtgaaagcc tgacgtggac aatagctaac
     4141 gaaaatggcc tgatgcgata tgtttatcag gccacgtctt aatggaatgc tttggatttg
     4201 caaattttgt agaccggata aggaattcac gccgcatccg gcatcaacaa agtgcacgtt
     4261 gttatccggt tatcaagcca aagcgccgta gctgacggca atgaaagcga gcgaggaata
     4321 tcaccgtggt taatgcgcca agcagcgcgc agaacatatc ggattgcgtg tcccactggt
     4381 cgccctgggt gccgagaaaa tcatccgctc cctggcccat tgccagcgcc gcccaccact
     4441 cgattaattc atacatcgcg cttatcgcca gcgccacgca acagaccaga aacgccacca
     4501 ttttacgtcc gcgaacgtac atcccgcgca caaggatctc tcgtgccacc agtgcaggca
     4561 ccagcccctg gaaaaaatgc cccagcttgt catacggatt acggctcaac cccagccatt
     4621 cctgcacctc aaaaccgatg gggactttcg cgtaggtgta ttgcccgccg accatcagga
     4681 tgatggcgtg aaagaaaatg agcgtataga gcagcggcgt taacggataa cgtttagcgg
     4741 tggcaagcag tagcggcacg acaataatca ccggtgtcac ttccatcagc caggtgagtt
     4801 tgtcatgggc cgaaatgccg gtataaatca ggattaacgt tagcgccagt gcgccggtgt
     4861 taagaattaa cggcttgagt gtgcgggtca tggtgagttc gcaagtcagg gaaaatactg
     4921 actattcacc agcaagcgtg aaattacaat cgcaaaagag gtggcccagg gggatcacct
     4981 ggcagcatgc tgccaggcgc tgggccgaag aggttactta gtgcagttcg cgcactgttt
     5041 gttgatgatt tgctggaaga agtcgttacc tttgtcatcg accaggataa acgccgggaa
     5101 atcttctact tcgattttcc agatagcttc catacccagc tccggataag cgacgcattc
     5161 cagatgcttg atgctctgct gcgccagtac cgccgccgga ccgccgatgc taccgaggta
     5221 gaagccgccg tgtttatgac acgcgtcggt aacctgctga ctgcggttac ctttcgctag
     5281 catgatcatg ctgccgccgt gggattgcag cagatccaca taggagtcca tacgacctgc
     5341 ggtggttggg ccaagtgagc ctgatggata accggcaggg gttttcgccg gacccgcgta
     5401 gtagatcggg tgatctttga tgtactgcgg aagttcttta ccggcgtcaa tcagctcttt
     5461 cagcttggcg tgtgcaatat ctcggcccac gataatggtg ccggtgagcg acaaacgagt
     5521 ggaaaccgga tattgcgaaa gctgggcgag gatctctttc atcgggcggt taaggtcgac
     5581 cttcaccgct tcgccttcac cggcctggcg cagttcttgt ggaatgtact gacctggatg
     5641 gtgttccagt ttttcgatcc agataccttc gcggttgatt ttcgctttaa tgttacggtc
     5701 agcggagcag gagacgccca tgcctaccgg gcaggatgcg ccgtgacgtg gcagacggat
     5761 aacgcgaatg tcgtgcgcga agtatttacc gccaaactgc gcgccaagac cgagtttctg
     5821 ggcctcttcc agcagttcct gttccagctg gacatcgcgg aacgcctgac catgttcgtt
     5881 cccttccgtc ggcagttcat cgtaatagtg agcgcttgct aacttaacgg ttttcaggtt
     5941 ggtttccaca gacgtaccgc caatcacaaa cgcgatatgg tacggcgggc aggctgcagt
     6001 accgagggta cgcattttct cgacgaggaa gtttttcagt ttgccgggag tcagcagggc
     6061 tttggtttcc tggtagagat aggttttgtt ggcagagccg ccgcctttcg caacgcaaag
     6121 gaatttgtac tcatcgccat ctaccgcgta caggtcgatt tgcgcaggca ggttagtgcc
     6181 ggtgttgacc tctttgtaca tgtccagcgc cgcgttttgc gagtagcgca ggttatcttc
     6241 gatataggtg ttatagacgc ctttcgacag cgcttcttca tcaccgccac cggtccacac
     6301 gcgctggcct tttttaccga cgatgatcgc ggtgccggta tcctggcagg tcggcagcac
     6361 gcctttggcg gcgatttcgg agtttcttaa gaattgcagt gccacgtact tgtcgttttc
     6421 gctggcttct ggatcgtgaa gaatagccgc aacctgtttc tggtgtgccg gacggagcat
     6481 gaaagaagcg tcgtggaagg cttgctgcgc cagcagggtc agggcttctg gttccacttt
     6541 caggatggtt tcgccgtcga agtcggcaac gctaacgtaa tcggaagtga gtagatagta
     6601 ttcggtattg tctttcccca tcgggaaagg tgcctggtag ataaagggtt tgtttgacat
     6661 agcttccagc ctgtaactct gtatttgtta ttcgaaaatg gcgtgccgcg tagtaacggg
     6721 cggcacgcaa agtgcattta taagaacccg tacatcgcgg cgaagatcca gccgaagacg
     6781 cacgatacgc tcacaccaat caaccccggc agaataaagc tgtggttgat gacgaagcga
     6841 ccgatgtggg tggtgccgga acggtcaaac tgaatcgctg ccagatcgct cggataagtc
     6901 ggcaggatgt aataaccgta gcaagccggt gctgaagcca cgatgtatgc cggatcaacg
     6961 ccgatcgcta gcgcgaccgg aacaatcgcc gccagcgccg cagcctgaga gtttacaaac
     7021 ttggaaacca gcagcagaac aatggcatag gcccacggat actctttcac catttcaccc
     7081 agtacgccct gaatttcaga catatgcgca ccgaacatgg tttctgccat ccatgcgata
     7141 ccgtacaccg ccacgatggc gatcataccg gaacggaaga cttcgttttt tgagatagac
     7201 gcgggattgg ttttggtcag gataataatc agcgccccgg tcagcagcat aaacatctga
     7261 ataaccagta ccatcgacag cggtttgccg ccgaaggatg gacgcaggtc cgaatcagca
     7321 ccaagaagtg cgactacagc gattgccccg aggaaaatcc acattgccag ccagttgctt
     7381 ttcggcagtt ttttatccag cagcgtcgcg gtatcaccgt aaacatactc acggttttcc
     7441 ggtacggaga tgaatttctg gaactcttcg tctttatcca gatctttacc gcggaaccag
     7501 ctgaagatac cgatcgccag gataccgatt aacgtcgatg gaatggtgat tgccagcagg
     7561 tcgaggaact caagatggcg accatcaaag gtgacattac ccagcattgc aaccagagac
     7621 acgaccgcaa ccgacaccgg actggcgata atccccatct gtgcaccgat agaacttgcc
     7681 gccatcggac gttccggacg gatgttgttc ttaatggcga cgtcgtagat gatcggcaga
     7741 atggtgtaaa ccacatgacc cgtaccgcaa agaatggtca gcgtacaggt cacaaacggc
     7801 gcgacaattg agacatattt cgggttgcgg cgcagcagct tctcggcaat ttgcagcatg
     7861 acatcaagac cgcccgaagc ttgcaaggtc gccgatgccg ccaccaccgc aatgataacc
     7921 agcatgacat caactggtgg tttacctggc tgaaggtgga agacgaagac cagaatgacc
     7981 agaccgatac cgcctaataa acccagcgcg ataccaccct ttctggcacc ataaaacaga
     8041 catatcagta ttatgataag ttggatagta aataacatgt gtgaaccctc gcgataatcc
     8101 tatttaaatt tttgctgaat agatcacagt cacgttctgt tttgtatgaa ctgttttcag
     8161 agcctgacta aatatccgtt tggtcgttac cggctttagc aaatacctca cagtgaatat
     8221 tggctgatta aagcagcggc aaatttatgc actgtgataa gcggcttttt cagagagaaa
     8281 aactctctgg cgatgggttt aaagtttgta ctgcgcagat gttaatgttt tgtttgctgt
     8341 tatcttattg atatttatcg gtctattttt tgttctcagg gcagatatca ttaggtttat
     8401 agattaatct gatctaccca tttgtgggta aaaatacaca taatgcgggt gacataatag
     8461 ttaattaact tttgttagcg ttttgaaatt aaaaacaccg ttcacctgaa gagatattaa
     8521 tttttagcga tgatggaggg ataattatat ttgatctggc acaagtttta ctgatgaagg
     8581 atgtaacttg taccaggggt attttgcatt acggtaataa ttattactta cagataacca
     8641 gcgatccgtt attggcaata ttgtttcagt agtgagtagt gttctgcctg aatacggtaa
     8701 cggtaaactg gacgccccgt gacgccataa tggatactgg tgaacaagat gtggcagttg
     8761 accagccaga taaggtattt acggcaggaa acacgcgaaa tgttaacctc gttggctagc
     8821 tcgtcggttg aaaactcata gtcctgatgc gcgtcaatcc actggcacag tgtgcgtaac
     8881 gtctgcggcg ttaagccttt tggcaagcga cgaggatcct gctcgttgga gctgctgccg
     8941 tggattagct gatcaagctc ggcctggtca taatactgat gtttttccag cgccattttc
     9001 ttttgccgcc agccggtgag cgcctcttca aagcgggaag cctggaaggg tttgatcagg
     9061 taatccacga caccgtaatg cagcgaatct ttaatggttg ccgcatcggc tgcggaggag
     9121 atgacaatca catcactttt gcaacgcgcg ttatgcagga caggcagtaa atcgagcccg
     9181 ttctcttttt gcatatagat atcgagcaat atcaggtcga taggcgtatc gctattgaag
     9241 ataatctctt tggctttctc cagcgtcgag gctgttccac agcattgaaa gcctgggatt
     9301 tgtgctacgt atcggcgatt cagctccgcg accattgcgt cgtcatcgat aattaataca
     9361 ttgatcatct gttcgacctc tccccgtccc agggtatctg gacaaaaaat tgtgtgaaaa
     9421 tcccgggttc cgattccacg gcgatgctgc cgccgagatt ttctacctgt tgtttgacaa
     9481 gtgctaaacc gacgcctcgc tcgcttcctt ttgtcgagac acctttgtca aaaatgtgat
     9541 cgattttgtc gggtgcgatc cccggtccat catcgttaac ttcgcagtgc agccagccgt
     9601 gacggtagtg caatgttacg ctaatttcgc caccgggttc cggccctaat gcttccagcg
     9661 cgttttctat cagatttccc aatgtggtaa tcagcgtcgc gacctggtcc tcactgccgc
     9721 tgtctggcag ctggctttca ctgtttaaaa tcagcgtatg gcctaaatcg gtcgcgcggt
     9781 taatcttgct gattaaaaaa ccagcgataa ccggagattt gatcttaccc agcagagagc
     9841 caatctcttc ctgatagtta ttggctgttt tgagaatgta atcttccaac tgcttataac
     9901 tcttcagatg caataatccg agaatcacat gcaatttatt cataaattcg tgggatcgtt
     9961 cacgaagtgc atcagcatag ttgaccagac cgtcgagtcg ctgcatcagt ttacgaactt
    10021 cagttttgtc cctgaaggtt gaaatggcac cgatgataac gccattactg cgcaccggaa
    10081 cggtgttgat cagtaatagc cggtctttaa tcgtaatctc ttcgtcgcgg cgcggcgtac
    10141 cgtcgcgtaa cacttccgag acatctacca cctgtgacca tgagtggctg agcgtcgaca
    10201 gtttctcatc gtcctgcgac ttacggtaat tcagcaattc ttgtgcggca tcgttgatca
    10261 gcgtgacctc gccgcgatcg tccacggcaa cgacgccttc tttgatagac tgcaacatgg
    10321 cctggcgttg ctcaaacagc gtggagattt cgtagggttc caggccgaaa aggatttttt
    10381 tcagtacctt aaccagaatg caggtgccaa tcagtccgac cagcatgcca aataataccg
    10441 accagataat gctccagcga ctgtcattga tctgttgggt cacgcggctt aactcaaggc
    10501 cgatcgccac cacgccaatt tgtttatgat tttcatcgta gatgggggta aatacgcgta
    10561 aagcctgcgc cagaaaaccg cgattgatag cgacattttc ttcgccattc agcgctttaa
    10621 ggatgtcatc acctttaaat ggctgaccaa tacgctgggc ttcaggatgc gagtagcgaa
    10681 gactttgcat atcggtaacg acaataaaca gcagatcgtt gcgtttgcgc acggcttccg
    10741 cgatggcctg gatgccgctc tcctgcggtt ttttctgcaa gccctgacgg atttccggcg
    10801 agtcggcgag agtacgcgcc actgccagtg ccttgttggc tagcccgtct cgcgtcatat
    10861 cactgatttg cgagaagtaa atcagatgca ccaccaatag caccgagaac agtaccgcgc
    10921 tgaccattaa gatcactgtg gtactcaatt tcatcggacg tttgcgtaac atgcggtagg
    10981 gcaatgaatg tctcatcagc ttccttgtgt gacaaatttc ttaagcatta tctctgatga
    11041 ggcgggtaat tcaaagggag taagaatgat tggctatata ggggaagaga ctctggcaac
    11101 ggaaactgcc agtgctgtat gaagattcca gggctatgct tatagtgaaa accatactga
    11161 tgagagaggg aaggtcatgg atcaggcgct actggacggg ggttatcgct gttataccgg
    11221 cgaaaagatc gatgtctatt tcaacactgc gatatgtcag cattctggca attgcgtacg
    11281 tggcaacggc aagttattta atctcaaacg aaagccgtgg atcatgccgg atgaagtcga
    11341 cgttgccact gtggttaaag tgattgatac gtgcccgagt ggtgcgctga aataccgtca
    11401 taaataagcg agggtaaaat ggaaatacgc gaaggccaca ataaatttta cattaatgac
    11461 gaacaaggca agcaaatcgc tgaaattgtc tttgtgccga ccggagagaa tttagcgatt
    11521 atcgaacata ccgatgtcga tgaaagcctg aaagggcaag ggattggtaa acagctggtt
    11581 gcgaaagtcg tggaaaaaat gcgtcgggaa aaacgaaaaa ttatcccatt atgtccattt
    11641 gcgaaacacg aatttgataa aacgcgggag tatgatgata ttcgcagttg atggaagagt
    11701 acagattcac gatatttttc atgctctccg cggtgtgatg caggagagca tctgaagggt
    11761 agggggatgc acaaagaatg ggcagagagc gcgttttttt gtcccaagtc atccccttta
    11821 ctgagcaaaa aaaagaatat ctcctatatg agaatcatca atcggggtta ataaattttg
    11881 cgtccccaga gtgttcaata tttataggag tcatattatg gaaggtaaaa acaagttcaa
    11941 tacttatgtt gtttcttttg atta
//
LOCUS       DBFIFM010000136        11886 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.136, whole genome shotgun sequence.
ACCESSION   DBFIFM010000136 DBFIFM010000000
VERSION     DBFIFM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.136"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            125..328
                     /locus_tag="N7C74_003290"
     CDS             125..328
                     /locus_tag="N7C74_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HGU2433106.1"
                     /translation="MSSIFLTENELQTLTGCKYASHQRNWLIKNGLPFYTNRSGKPIV
                     SRELFTCIKTLPPREAEPDFGAI"
     gene            328..1407
                     /locus_tag="N7C74_003291"
     CDS             328..1407
                     /locus_tag="N7C74_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2433107.1"
                     /translation="MGRRRKNPEHEKLPPKVYPNKYSVWKPTSRESVTLTAIEDGLAA
                     LWKKYEETVNHRDRAMTFGRLWEKFLASAYYSELSPRTQKDYLQHQKKLLAVFGKVLA
                     DSVKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAKN
                     RERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGKT
                     GKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNHAGKISG
                     ILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPENI
                     PRKYSKKIFQVIPSVIFVTDLMMCK"
     gene            complement(1416..1505)
                     /locus_tag="N7C74_003292"
     tRNA            complement(1416..1505)
                     /locus_tag="N7C74_003292"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1469..1471),aa:Ser,seq:cga)
     gene            1599..2396
                     /gene="mtfA"
                     /locus_tag="N7C74_003293"
     CDS             1599..2396
                     /gene="mtfA"
                     /locus_tag="N7C74_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HGU2433108.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2497..2572
                     /locus_tag="N7C74_003294"
     tRNA            2497..2572
                     /locus_tag="N7C74_003294"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2530..2532,aa:Asn,seq:gtt)
     gene            2886..10868
                     /locus_tag="N7C74_003295"
     CDS             2886..10868
                     /locus_tag="N7C74_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HGU2433109.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLITQLVFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYTLGALESAQIVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSENNLTPPPGNSSGNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAEGWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVDARRSLAGSRFDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNFSNREQSMVVVQAPTLSQKDSSVSLSSQTLSADSHSTATLTFIAHDAAGNPV
                     IGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNTAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVKVTFNVNSAAAKLSQTEVNSHDGIATATLTSLKNGDYT
                     VTASVSSGSQANQQVIFIGDQSTAALTLSVPSGDITVTNTAPLHMTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNSGKGMTDSNGTAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPAD
                     TQLSLNARNTNENGIAEVTLKGTVLGVHTAEAILLNGNRDTKIVNIAPDASNAQVTLN
                     IPAQQVVTNNSDSVQLTATVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQAN
                     GEAHVTLKGKKAGTHTVTATLGNNNASDAQPVTFVADKDSAVVVLQTSKAEIIGNGVD
                     ETTLTATVKDPFDNAVKDLQVTFSTNPADTQLSQSKSNTNDSGVAEVTFKGTVLGVHT
                     AEATLPNGNNDTKIVNIAPDASNAQVTLNIPAQQVVTNNSDSVQLTATVKDPSNHPVA
                     GITVNFTMPQDVAANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLSNNNTSDS
                     QPVTFVADKTSALVVLQISKNEITGNGVDSATLTATVKDQFDNEVNNLPVTFSTASSG
                     LTLTPGESNTNESGIAQATLAGVAFGEQTVTASLANNGASDNKTVHFIGDTAAAKIIE
                     LTPVPDSIIAGTPQNSSGSVITATVVDNNGFPVKGVTVNFTSNAATAEMTNGGQAVTN
                     EQGKATVTYTNTRSSIESGARPDTVEASLENGSSTLSTSINVNADASTAHLTLLQALF
                     DTVSAGDTTNLYIEVKDNYGNGVPQQEVTLSVSPSEGVTPSNNAIYTTNHDGNFYASF
                     TATKAGVYQVTATLENGDSMQQTVTYVPNVANAEISLAASKDPVIANNNDLTTLTATV
                     ADTEGNAIANSEVTFTLPEDVRANFTLGDGGKVVTDTEGKAKVTLKGTKAGAHTVTAS
                     MAGGKSEQLVVNFIADTLTAQVNLNVTEDNFIANNVGMTRLQATVTDGNGNPLANEAV
                     TFTLPADVSASFTLGQGGSAITDINGKAEVTLSGTKSGTYPVTVSVNNYGVSDTKQVT
                     LIADAGTAKLASLTSVYSFVVSTTEGATMTASVTDANGNPVEGIKVNFRGTSVTLSST
                     SVETDDRGFAEILVTSTEVGLKTVSASLADKPTEVISRLLNAKADINSATITSLEIPE
                     GQVMVAQDVAVKAHVNDQFGNPILNESVTFSAEPPEHMTISQNIVSTDTHGIAEVTMT
                     PERNGSYMVKASLANGSSYEKDLVVIDQKLTLSASSPLIGVNSPTGATLTATLTSANG
                     TPVEGQVINFSVTPEGATLSGGKVRTNSSGQAPVVLTSNKVGTYTVTASFHNGVTIQT
                     QTIVKVTGNSSTAHVASFIADPSTIAATNSDLSTLKATVEDGSGNLIEGLTVYFALKS
                     GSATLTSLTAVTDQNGIATTSVRGAITGSVTVSAVTTAGGMQTVDITLVAGPADASQS
                     VLKNNRSSLKGDFTDSAELHLVLHDISGNPIKVSEGLEFVQSGTNAPYVQVSAIDYSK
                     NFSGEYKATVTGGGEGIATLIPVLNGVHQAGLSTTIQFTRAEDKIMSGTVLVNGANLP
                     TTTFPSQGFTGAYYQLNNDNFAPGKTAADYEFSSSASWVDVDATGKVTFKNVGSKWER
                     ITATPKTGGPSYIYEIRVKSWWVNAGDAFMIYSLAENFCSSNGYTLPLGDHLNHSRSR
                     GIGSLYSEWGDMGHYTTEAGFHSNMYWSSSPANSNEQYVVSLATGDQSVFEKLGFAYA
                     TCYKNL"
     gene            complement(11130..>11886)
                     /locus_tag="N7C74_003296"
     CDS             complement(11130..>11886)
                     /locus_tag="N7C74_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HGU2433110.1"
                     /translation="LAKNITQQYGTPDLCIEAMRKKNLKTMLFISQLKAKTNFQVVGL
                     TMKCYSPVCKNASRMDQKLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREE
                     MRSLGSYIALNLEGSSQDRTFSLSIAENLIEKIQSETDIPIVIVHGPKGEDKARVLVD
                     CYNNVYRLSLSPSIKRSAAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPA
                     MADVSESVVVGQKIDNISLDEFAKALKSVLARI"
BASE COUNT         3379 a         2829 c         2866 g         2812 t
ORIGIN      
        1 cgttaccagg caaaatacgt ttcagccatc tggcaaaaac tgctcccgag tcaggctgat
       61 ttttaatgtt caacccggat cgccgctaac ggcatatagt tagcggcggt tgtgagatat
      121 agctatgagc agcatatttt taaccgaaaa tgaattgcaa acgttaacgg gctgcaaata
      181 tgcaagccac cagcgcaact ggttaataaa aaatgggctt ccgttctata ccaatcgtag
      241 tggaaaaccg atcgtcagtc gggaactgtt tacctgtata aaaactttac caccacgtga
      301 ggctgaacct gatttcggtg cgatctaatg ggaagacgaa ggaaaaatcc cgaacacgaa
      361 aaattaccgc caaaggtata ccccaataaa tatagtgtat ggaaaccgac atccagagaa
      421 tctgtaacct taactgcaat cgaggatggt ttagccgcat tatggaaaaa gtatgaagaa
      481 acggttaacc atcgcgatcg cgcaatgaca tttgggcgtt tgtgggaaaa atttctcgcc
      541 agcgcctatt acagcgagct tagtcctaga actcaaaaag attatctgca acatcaaaaa
      601 aagctgctgg ccgtattcgg taaggtgctg gccgattctg taaaaccaga gcacatcaga
      661 cgatacatgg acaaaagagg cgagcagagt aaaacgcagg caaaccatga aaaaagcagt
      721 atgtcgcgcg tttatagttg ggggtatgag cgaggatacg tgaaggctaa cccatgtgca
      781 ggtgtaagta aattcaaggc caaaaaccgc gaacgatatg taaccgacaa agaataccag
      841 gcagtattaa gcgttgcacc tcttcctgtt tttatcgcaa tggaaattgc ctatctgtgt
      901 gcagcgaggg tttccgatgt gttatcgctg aaatgggagc agattggaaa cgacgggatc
      961 tttatccagc aagggaaaac agggaaaaaa cagataaaag catggagtcc acgattacag
     1021 gcggcgatcg aaaaagcaaa acagttacca acatccgcct atgtaatcag caatcaatac
     1081 ggcaaccgat atatgtacaa aggctttaac gaaatgtggg tagaagcaag aaatcacgca
     1141 ggcaaaattt caggtatttt aaccgacttc acctttcatg atctgaaggc gaaaggaatt
     1201 tcagactatg aaggaagcag ccgggataag caacttttct ctggtcacaa aaccgaaggg
     1261 caagtgctaa tctatgacag gaaggttaaa gtttcaccaa cacttgatgt cccgttacct
     1321 gaaaatattc caagaaaata ttccaagaaa atattccaag taattccaag tgtgattttt
     1381 gtcactgact taatgatgtg taagtgattg aattttggcg gagagagggg gatttgaacc
     1441 cccggtggag ttgcccccac tccggttttc gagaccggtc cgttcagccg ctccggcatc
     1501 tctccgttca gatggttgcc atgatgccag gaaatttggc attttaacag tccctgtccg
     1561 tgcaattttg ttcaagtgac gagtttgcga gcaaaacgat gattaagtgg ccctggaaag
     1621 tacaagaatc agcacatcaa actgcccttc cctggcagga agcactatcg atcccccttt
     1681 taacgtgtct gacggaacag gaacaaagca aattagtcac tcttgccgaa cgttttttac
     1741 agcaaaagcg gcttgttcct ttacagggct ttgaactgga ttcattaaga agctgccgga
     1801 tagcacttct attttgccta cccgttctgg agttaggact ggaatggctg gatggttttc
     1861 atgaagtctt aatttatcct gcgccatttg tggtcgatga tgaatgggaa gacgatatcg
     1921 gtctggtgca taaccaacgt attgttcagt caggtcagag ctggcagcaa gggcctatcg
     1981 ttttgaactg gttggatata caagattctt ttgatgcttc tggttttaac ctgattattc
     2041 atgaagtcgc tcataagctg gacacccgta acggcgatcg cgccagcgga gttcccttta
     2101 ttccgttgcg tgaggttgct ggctgggaac acgatcttca tgctgcaatg aacaacattc
     2161 aggaagaaat cgaattggtt ggcgagaatg cggcgagcat tgatgcttat gctgccagtg
     2221 atcctgctga atgttttgcc gtactttctg aatatttctt tagcgcccca gaactttttg
     2281 ctcctcgttt cccttcattg tggcaacgtt tctgccaatt ttatcaacaa gatcctttgc
     2341 agagactgca tcacgctaat gatacagact cgttttcggc gacgaatgtt cattaattaa
     2401 caactttgca gattaattaa ccaattgaaa tgacttatga aatttagtgt tgacagacaa
     2461 ggtaccgcta agtaatatgc gccccgttca cacgattcct ctgtagttca gtcggtagaa
     2521 cggcggactg ttaatccgta tgtcactggt tcgagtccag tcagaggagc caaattctaa
     2581 aaattcgctt ttttagcgca atgtcactga ccttagttga acattgtttt ttaacggata
     2641 gcgggttttt aacatcttaa gcgccctcga cctttatggt tgagggcgtt ttgctatgaa
     2701 cgccatcacc attttcccct cgattataaa acttgagtta ttcagtagtc tcccctcttg
     2761 caactcacat ccaaaactgc ctaacgaaaa gttattaatt ttcaatcata ttgctatcag
     2821 gatttacatt ttttcgctgt gctagaaagg gcgcatttat gttagctcgt tcagggaagg
     2881 taagcatggc tacgaagaag agaagtggag aagaaataaa tgaccgacaa atcttatgcg
     2941 ggatgggaat taaactacgc cgcttaactg cgggtatctg cctgataact caacttgtgt
     3001 tccctatggc tgcggcagca caaggtgtgg taaacgccgc aacccaacaa ccagttcctg
     3061 cacaaattgc cattgcaaat gccaatacgg tgccctacac ccttggagcg ctggaatcgg
     3121 cccaaatcgt tgccgaacgt ttcggtattt cggtggctga gttacgcaaa ctcaaccagt
     3181 ttcgtacgtt tgctcgaggt tttgataatg tccgccaggg tgatgaactg gatgtcccgg
     3241 cacaagttag tgaaaataat ttaaccccgc caccgggtaa tagcagtggc aaccttgagc
     3301 aacagatagc cagtacttca cagcaaatcg ggtctctgct cgccgaggat atgaacagcg
     3361 agcaagcggc aaatatggcg cgtggatggg cctcttctca ggcttcaggc gcaatgacag
     3421 actggttaag ccgcttcggt accgcaagaa tcacgctggg cgtggatgaa gattttagcc
     3481 tgaagaactc ccagttcgat tttctccatc cgtggtatga aacgcctgat aatctctttt
     3541 tcagtcagca tactctccat cgtactgacg agcgtacgca gattaacaac ggcttgggtt
     3601 ggcgtcattt cactcccaca tggatgtcgg gcatcaactt ctttttcgac cacgatctta
     3661 gccgttacca ctcccgcgcc ggcattggcg cggagtactg gcgcgactat ctaaaattaa
     3721 gcagtaacgg ctatttgcga ctgaccaact ggcgcagcgc acctgaactg gacaacgatt
     3781 atgaagcacg cccggccaat ggctgggatg tacgcgcaga aggctggcta cccgcctggc
     3841 cgcaccttgg cggtaaactg gtctatgaac agtattatgg cgatgaagtg gccctgttcg
     3901 ataaagatga tcggcaaagt aatcctcatg ccataaccgc tggacttaac tataccccct
     3961 tcccgctgat gaccttcagc gcggagcaac gccagggtaa acagggcgaa aatgacaccc
     4021 gttttgccgt cgattttacc tggcaacctg gaagcgcgat gcagaaacag cttgacccga
     4081 atgaagtcga tgcacggcgt agccttgcag gcagccgttt tgatctggtg gatcgcaaca
     4141 acaacatcgt tctggaatat cgcaaaaaag aactggttcg cctgaccctg acagaccccg
     4201 tgacagggaa gtcaggagaa gtgaaatcac tggtttcgtc gctacaaacc aaatatgccc
     4261 tgaaaggcta taacgtcgaa gccaccgctc tggaagctgc cggtggtaaa gtggttacaa
     4321 cgggtaaaga tattctggtt accctgccgg cgtaccggtt caccagtacg ccagaaaccg
     4381 ataacacctg gccgattgaa gtcaccgctg aagatgtcaa aggcaatttt tcgaatcgtg
     4441 aacagagcat ggtagtcgtt caggctccta cgctaagcca gaaagattcc tcggtatcgt
     4501 taagtagcca gacgttgagc gcggattccc attcaaccgc cacactgact tttattgcgc
     4561 atgatgcagc aggtaatcct gttatcgggc tggtgctctc gacgcgtcac gaaggtgttc
     4621 aggacatcac cctttctgac tggaaagata atggtgacgg aagctatacc cagatcctga
     4681 ccacaggagc gatgtctggc acgctgacgc tgatgccaca gctgaacggt gtggatgcgg
     4741 ctaaagcccc cgccgtggtg aatatcattt ctgtttcgtc atcccggact cactcgtcaa
     4801 ttaagattga taaggaccgt tatctctccg ggaatcctat cgaggtgacg gtagaactga
     4861 gagatgaaaa tgacaaacct gttaaggagc aaaaacagca actgaatacc gcagtcagca
     4921 tcgacaacgt gaaacctggt gtcactacag actggaaaga aaccgcagat ggcgtctata
     4981 aggcaaccta taccgcctat accaaaggca gtgggcttac tgcgaagctg ttaatgcaaa
     5041 actggaatga agatttgcat accgctggat ttatcatcga cgccaacccg cagtcagcga
     5101 aaattgcgac attatctgcc agcaataatg gtgtgctcgc caatgagaat gcagcaaaca
     5161 ccgtctcggt caatgtcgct gatgaaggaa gcaacccaat caatgatcat accgtcacgt
     5221 ttgcggtatt aagcggatcg gcaacttcct ttaacaatca aaacaccgca aaaacggatg
     5281 ttaatggtct ggcgactttt gatctgaaaa gtagtaagca ggaagacaac acggttgaag
     5341 tcacccttga aaatggcgtg aaacaaacgt taatcgtcag ttttgtcggc gactcgagta
     5401 ccgcgcaggt tgatctgcag aagtcgaaaa atgaagtggt cgctgacggc aatgacagtg
     5461 ccacaatgac cgcgacagtt cgggatgcaa aaggcaacct gctcaatgac gtcaaggtca
     5521 ccttcaatgt caattcagca gcagcgaaac tgagccaaac cgaagtgaat agccacgacg
     5581 ggatcgccac agctacgctg accagtttga aaaatggtga ttatacggtt acggcctctg
     5641 tgagctctgg ttctcaggct aatcaacagg tgatttttat cggtgatcaa agtactgctg
     5701 ccctgaccct cagtgtgcct tcaggtgata tcaccgtcac caacacagct ccgctacata
     5761 tgactgcaac cttgcaggat aaaaatggca atccactaaa agataaagaa atcaccttct
     5821 ctgtgccaaa cgacgtcgca agtcggttct cgattagcaa cagcggaaaa ggcatgacgg
     5881 atagcaacgg gactgcaatc gcctccctga ccggcacgtt agcgggcacg catatgatca
     5941 cggctcgtct ggctaacagc aatgtcagcg atacacagcc aatgacgttt gtggcggata
     6001 aagacagagc ggttgtcgtt ctgcaaacat cgaaagcgga aatcattggg aatggcgtgg
     6061 atgagacgac tctgacagca acagttaaag atccttttga taacgtggtt aaaaatcttt
     6121 cagtagtctt ccgcacctcc cccgcagaca cgcaactgag tctgaacgcg cgtaatacta
     6181 atgagaacgg tattgccgaa gttaccctta agggcacggt tttgggtgtt catacagccg
     6241 aagccatact gcttaacggc aacagagata cgaaaatcgt caatattgcg cccgatgcca
     6301 gcaacgcgca ggtcaccctg aacatccctg cacaacaggt ggtgacgaat aacagtgaca
     6361 gcgtgcagct gacggcgacg gtgaaagacc cgtcgaatca tccggtggcg ggaataacgg
     6421 tgaacttcac catgccacag gacgtggcgg caaactttac ccttgaaaat aacggtattg
     6481 ccatcactca ggccaatggc gaagcgcatg tcaccctcaa aggcaaaaaa gcgggcacgc
     6541 atactgtgac cgccacgctg ggtaacaata atgccagcga tgcgcaacca gtcaccttcg
     6601 tggcggataa ggacagcgcg gttgtcgttc tgcaaacatc gaaagcggaa atcattggga
     6661 atggcgtgga tgagacgact ctgacggcaa cagtgaaaga tccttttgat aacgcagtaa
     6721 aagatctaca ggtcaccttc agtaccaacc ccgcagatac tcaacttagt cagagcaaaa
     6781 gcaatactaa cgacagtggt gtggccgaag ttacctttaa gggcacggtt ttgggtgttc
     6841 atacagccga agccacactg cctaacggca acaacgatac gaagatagtc aatattgcgc
     6901 ccgatgccag caacgcgcag gttacgctga acatccctgc tcaacaggtg gtgacgaata
     6961 acagcgacag cgtgcagctg acggcgacgg tgaaagatcc gtcgaatcat ccggtggcgg
     7021 gaataacggt gaacttcacc atgccacagg acgtggcggc aaactttacc ctcgaaaata
     7081 acggtattgc catcacccag gccaatgggg aagcgcatgt cacgctcaaa ggtaaaaaag
     7141 cgggtacgca tacggttacc gcaacgctga gtaataacaa taccagtgat tcacagccgg
     7201 taacgtttgt ggcggacaaa acctcggctc tggttgttct tcagatatca aaaaatgaga
     7261 tcacaggtaa tggcgtcgat agcgcaacgc taactgcaac ggtcaaagat cagttcgaca
     7321 atgaggtgaa caatcttccg gtaacattca gcacagcttc ttcaggcctc accctgaccc
     7381 caggggaaag taataccaat gagtctggca tcgcgcaggc cactctcgca ggcgttgcct
     7441 ttggtgagca gacggtcact gcatcactgg ctaataatgg tgccagcgac aacaaaactg
     7501 tgcattttat tggcgacaca gcggcggcaa aaattatcga gttgacgcct gtcccagaca
     7561 gcataatcgc aggtaccccg cagaacagct ccggcagcgt catcaccgcc acagtcgttg
     7621 ataataatgg ctttccggtg aaaggtgtga ctgtgaactt caccagcaac gcagcgacag
     7681 ccgaaatgac gaatggcggt caagccgtga cgaacgaaca gggtaaggct accgtcactt
     7741 ataccaatac ccgctcctcg atagaatcag gagcgagacc ggataccgtt gaggccagtc
     7801 tggaaaatgg tagctccacg cttagcacat caattaatgt caacgctgat gcgtctacgg
     7861 cacatctcac cttgctacag gcactttttg atacagtctc cgcaggcgac actaccaatc
     7921 tgtatattga ggtgaaggat aattacggca acggagtacc ccagcaggag gtaaccctca
     7981 gcgtttcacc aagtgaaggt gtgaccccca gtaataacgc tatatatacg accaatcacg
     8041 acggcaattt ttacgcaagc tttaccgcta caaaagccgg ggtataccaa gtgacggcaa
     8101 ccctcgaaaa tggcgattcg atgcaacaaa cagtgaccta tgtgccgaac gtagcgaatg
     8161 ctgaaatctc gctggcagcc tcgaaggatc cggtaattgc caacaataac gatctcacga
     8221 cactaacagc aacagtcgct gatacagagg gcaatgcgat agccaacagt gaggtaacat
     8281 ttactctgcc ggaagatgtg agggcgaact tcacgctggg cgatggcggt aaagtggtta
     8341 ctgatactga aggcaaagcg aaagtcacgc tgaaaggtac aaaagcaggc gctcatactg
     8401 ttacagcatc gatggctggc ggtaagagtg agcagttggt ggtgaacttt attgcggata
     8461 cactcactgc gcaggttaat cttaacgtta ccgaggacaa ttttatcgct aataacgtcg
     8521 ggatgaccag gctgcaggca acagtgactg atggaaacgg caacccgtta gccaatgagg
     8581 cggtgacatt cacgctaccg gcagatgtga gcgcaagctt tactctcgga caaggcggtt
     8641 ccgccattac tgacatcaac ggcaaggctg aagttacact gagcggtaca aaatccggca
     8701 cctaccccgt gacagttagc gtgaacaatt atggtgtcag tgatacgaaa caggtgactt
     8761 tgattgccga tgctggtacc gcaaaactag cctccttaac ctctgtatac tcattcgtcg
     8821 tcagcacgac cgagggcgcg accatgactg caagcgtcac tgacgctaac ggcaacccgg
     8881 tagaaggtat aaaagttaat ttccgcggaa cttccgtcac gctaagcagc accagcgttg
     8941 aaacggatga tcggggtttc gctgaaattc ttgtgacaag caccgaggtc ggactgaaaa
     9001 cagtttcagc ctctctggca gataaaccta ctgaagtcat ctcgcgatta ctgaatgcaa
     9061 aagcagatat taattctgca acgattacca gtctggagat acctgaaggt caggtcatgg
     9121 tcgcacaaga cgtagcagtt aaagctcacg tcaacgacca gtttggcaat ccgattctta
     9181 atgaatctgt aacattcagt gcagaaccac cagagcacat gaccatcagc caaaatattg
     9241 tctctactga tacgcatggt atagccgagg tcactatgac gcccgaaaga aacggttcgt
     9301 atatggtgaa agcatccctg gcgaatggat cctcttatga gaaggatctg gtggtaatcg
     9361 atcaaaaact gacactctcg gcgtccagcc cgcttatcgg tgtcaattcc ccaacaggtg
     9421 caactctgac ggcaacgcta acttctgcaa atggcactcc agtggagggt caggtcatca
     9481 actttagcgt aacgccagaa ggtgcgacgt taagtggcgg aaaagtgaga accaactctt
     9541 caggtcaggc tccagtcgtt ctgaccagca ataaagtcgg tacatatacg gtgactgcat
     9601 cgttccataa cggcgtaaca atacagacac agacaatcgt gaaagtcact ggcaactcaa
     9661 gcaccgccca tgttgctagc tttatcgctg atccatcgac tatagccgcc accaacagtg
     9721 atttaagtac cttaaaggca acggttgagg atggcagtgg taacctgatc gaaggtctca
     9781 ctgtgtactt cgccttaaaa agcggctctg ccacattaac gtcattaaca gcggtgacag
     9841 atcaaaacgg aatcgcgaca acaagcgtga gaggagcgat aacggggagc gtcacggtaa
     9901 gcgcagtcac gaccgctggt ggaatgcaaa cagtagatat aacgctggtg gcaggcccgg
     9961 cagacgcctc gcagtccgtc cttaagaaca atcggtcatc attgaaagga gactttaccg
    10021 atagtgctga gctacatctt gttctgcacg atatatcagg caatccgatc aaagtttctg
    10081 aagggctgga atttgtgcag tcaggtacca acgcgcccta tgtgcaagtt agtgcaattg
    10141 actacagtaa aaatttctca ggcgagtaca aagccactgt tacaggcggc ggagagggta
    10201 tcgcaacgct gatccctgta ttgaatggtg ttcatcaagc gggtctgagt accacaatac
    10261 aattcactcg cgcagaagac aaaataatga gcggtacagt gttagtcaat ggtgctaacc
    10321 taccgacaac tacattccct tcgcaggggt tcactggggc gtattatcag ttgaataatg
    10381 acaactttgc cccaggaaaa acggcggctg attatgagtt ttcaagctct gcctcctggg
    10441 ttgatgttga tgctaccggt aaagtgacat ttaaaaatgt cggcagcaaa tgggagagga
    10501 ttacggcgac gccaaaaaca ggcggcccta gctatatata cgaaatccga gtgaagagtt
    10561 ggtgggtgaa cgccggcgat gctttcatga tatacagcct tgctgaaaat ttttgcagta
    10621 gcaatggcta cacacttccc cttggagacc atttaaacca tagtcgttcc cgaggcatcg
    10681 ggtcactgta cagtgaatgg ggagatatgg ggcattacac gactgaagct ggttttcatt
    10741 caaatatgta ttggtcatcg agtcccgcaa actcaaacga acaatacgta gtttccctgg
    10801 caacaggtga tcaaagcgta tttgaaaagc ttgggtttgc ttatgcgaca tgttataaaa
    10861 acctctgatt ttccgttata atttcttaaa gagtgctcca ttcggggcac tcttttttac
    10921 attccctcta tagagcttgc ccttctctca cagaaaatga ataaataaaa atgcgatacc
    10981 gcaagaatcg cgttcgatgt gactatgatc gtatccggtg agtacagctt ttatccaaag
    11041 gctggttttc tgtgatgatt gaccagcctc agagtattga tgacaccacc gttcataaca
    11101 gatcggcatt actatgcata agtactgtct catattctcg ccaaaacact ttttaatgcc
    11161 tttgcgaatt catccagact tatattgtca attttttgcc caacgacgac tgactccgaa
    11221 acatcggcca ttgcgggcca tcgcgttttg taatcagcat aaattgcaac aacgggggta
    11281 ttataggcac ttgccatatg taatattgag gtgtcaggag ttattgcgat ataagcatct
    11341 tttatgattg ctgctgaacg tttaatcgag ggtgataagg ataaacggta gacattatta
    11401 taacaatcca ctaataccct ggctttatct tccccttttg gtccatgaac gatcactatt
    11461 ggtatatctg tttcactttg aattttttct attagatttt ctgcaatcga tagtgaaaat
    11521 gtacgatcct gcgagctacc ttctaaatta agcgcaatgt aagatcctaa cgatcgcatt
    11581 tcctcgcgca cctcatcgag tacatcctca cttagtggca attcatatat tggcctgact
    11641 gctggaaaac ccgcctcacg catcataaat gcccatgtca tgggtacggg agcccggagt
    11701 ttctgatcca tccgggacgc gttcttacac acaggggaat agcacttcat ggttaaacca
    11761 acaacctgaa aattcgtttt tgctttcaac tgactgataa ataacatcgt tttcaggttc
    11821 tttttacgca tcgcctctat acaaagatcc ggcgtaccgt attgctgtgt tatgttcttc
    11881 gctaaa
//
LOCUS       DBFIFM010000137        11877 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.137, whole genome shotgun sequence.
ACCESSION   DBFIFM010000137 DBFIFM010000000
VERSION     DBFIFM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.137"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..207
                     /locus_tag="N7C74_003297"
     CDS             <1..207
                     /locus_tag="N7C74_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433111.1"
                     /translation="AILGTVAVILNCHHQGARSVRAVNEESQPECQITGDRPVIKINN
                     TLWESNTAAAFLNRKSQFLYTTGK"
     gene            219..488
                     /locus_tag="N7C74_003298"
     CDS             219..488
                     /locus_tag="N7C74_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGU2433112.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            975..2912
                     /locus_tag="N7C74_003299"
     CDS             975..2912
                     /locus_tag="N7C74_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU2433113.1"
                     /translation="MAFKHYDVVRAASPSDLAKRITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGSISSPEEPGNRPTTSAPSVAPEYYYVIALAGQSNGMSYGEGLPLPDTF
                     DSPDPRIKQLARRSTVTPGGAVCKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPVNHAAQFGALVDKFRADLAD
                     MAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSSWARRGIISDRLATAIL
                     RHAGRVALNAGASSTVSEVRPSSPSGAEATGVTTLLSYLASESEGSLKVQGWSASGGR
                     AEVVSDAEGTGGKAVKLTKEAGKSSWVLEYAAGNGAALLQKGGQIRCRFKVSGALAAN
                     QYVMAFYWPVSSLPQGVALTGDGGNNLLAAFYIQTDAKDLNVMYHNAKVATNNLKLGT
                     FGAFDNEWHTLAFRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFAADKLHVTDITRG
                     ATYPVLIDSIAVEVNSTDTAA"
     gene            3047..3226
                     /locus_tag="N7C74_003300"
     CDS             3047..3226
                     /locus_tag="N7C74_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU2433114.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            3267..3539
                     /locus_tag="N7C74_003301"
     CDS             3267..3539
                     /locus_tag="N7C74_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU2433115.1"
                     /translation="MSEITSLVTAEAVKDVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            3616..3831
                     /locus_tag="N7C74_003302"
     CDS             3616..3831
                     /locus_tag="N7C74_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU2433116.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            3836..4369
                     /locus_tag="N7C74_003303"
     CDS             3836..4369
                     /locus_tag="N7C74_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU2433117.1"
                     /translation="MSRKFRYGLSAVVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GVGIWTICRGATRVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVAKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALSCWGIDR"
     gene            4643..5212
                     /locus_tag="N7C74_003304"
     CDS             4643..5212
                     /locus_tag="N7C74_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HGU2433118.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKRRNMVSLPLRKLAGWLQTINPNKVKPEIRGKVIQYQEEC
                     DDVLYEYWTKGFVVNPRRMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKSAHVS
                     KIRTLINEANLLIDFVLADTGKGKITKAD"
     gene            5212..5361
                     /locus_tag="N7C74_003305"
     CDS             5212..5361
                     /locus_tag="N7C74_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433119.1"
                     /translation="MEWWLMISDKLITLAKILCVIVGISFLVMLVAIFFSTAWRVLTL
                     SGLVG"
     gene            5369..5833
                     /locus_tag="N7C74_003306"
     CDS             5369..5833
                     /locus_tag="N7C74_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU2433120.1"
                     /translation="MNRVLCVVIIVLLVACGALSLGLNHYRDNAITYKEQRDKKVSEL
                     ELANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGYRSLRINATCPGP
                     VREATGTARVDNATGPRLADTAERDYFTLRERLITMQKQLEGTQKYINEQCR"
     gene            complement(5865..6158)
                     /gene="bor"
                     /locus_tag="N7C74_003307"
     CDS             complement(5865..6158)
                     /gene="bor"
                     /locus_tag="N7C74_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HGU2433121.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKQTAVAPKETITHHFFVS
                     GIGQKKTVDAAKICGGTENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            6567..7373
                     /locus_tag="N7C74_003308"
     CDS             6567..7373
                     /locus_tag="N7C74_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGU2433122.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPCNDAGSGDEKCAKNSEKNCANSAETKRIRGSRLL
                     PPSNAFSQRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGM
                     FADLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADR
                     ERINAARDKLRAETDILTNQRRGVVTPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            7354..9060
                     /locus_tag="N7C74_003309"
     CDS             7354..9060
                     /locus_tag="N7C74_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGU2433123.1"
                     /translation="MTFRKNEPRCDEPSEMTEAEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRSMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQEITASGLLLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYINKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPM
                     IVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDVVKRSNGEQVAHWFGHLDAELFAHLISQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            9060..11204
                     /locus_tag="N7C74_003310"
     CDS             9060..11204
                     /locus_tag="N7C74_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HGU2433124.1"
                     /translation="MKNEINTTAMKNDHGSTPRFSQRQLLSLCSDIDSQPLWRDAANK
                     ACAYYDGDQLAPEVIQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLIVMSDDPND
                     DTEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFKVST
                     VSRNEVFWDWLSREADLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TVTEGQPSPLMSAWEEYQSWDRQQNEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDNDLMEQL
                     ERPDGIIKLNPVRKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            11362..>11877
                     /locus_tag="N7C74_003311"
     CDS             11362..>11877
                     /locus_tag="N7C74_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433125.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYL"
BASE COUNT         3155 a         2697 c         3308 g         2717 t
ORIGIN      
        1 gcgatactgg ggactgtggc cgttatactg aattgccatc atcagggggc gcgttctgtt
       61 cgcgccgtga atgaagagag tcaaccagaa tgtcagataa ctggcgacag gcctgttata
      121 aaaataaaca atacattatg ggaaagtaat acagctgcag cgtttctgaa cagaaagtca
      181 cagtttttat atacaacggg taaataaagg agttaagcat gaagaagatg tttatggcgg
      241 ttttatttgc attagcttct gttaatgcaa tggcggcgga ttgtgctaaa ggtaaaattg
      301 agttttccaa gtataatgag gatgacacat ttacagtgaa ggttgacggg aaagaatact
      361 ggaccagtcg ctggaatctg caaccgttac tgcaaagtgc tcagttgaca ggaatgactg
      421 tcacaatcaa atccagtacc tgtgaatcag gctccggatt tgctgaagtg cagtttaata
      481 atgactgagg cataacctga ttcgtggtat gtgggtaaca agtgtaatct gtgtcacaat
      541 tcagtcagtt gacagttgcc tgtcagactg agcatttgtt aaaaaaattt cgcatggtga
      601 atccccctgt gtggaggggc gactggtgaa aaatccttgc ttgtgattca ttatcgacac
      661 gggttcggtg gtaccaggcc gaactcaccg ggaggcaccc ggcaccatgc agtatacaga
      721 gattaggcat ataccaaggc ctctcatagc aggggccttt ttacatgtaa aaaagcccga
      781 gtgggttcgg gcaattgcat gagatactcg ttttaataat cgaaatcatt ttaaccagga
      841 ttcataaggc tgcgcaactg cgcgaccttt ttcgaattgc gggctgtagt ctcccttctg
      901 ccattgtcct gtaacttccg gacttcagcc cgctccttat tttactcaca atattatccc
      961 ggccgggagg attcatggca tttaaacact atgatgttgt cagggcggcg tcgccgtcag
     1021 accttgcgaa acgaataact caaaaactga aggaagggtg gcagccttat ggtagtgcgc
     1081 tgatttcgac agctggttat ggtgcggagt tcatccagcc agttgtgagt gaggggagca
     1141 tctcatcacc agaggagcca ggcaaccgtc cgacgacctc agcgccttct gttgcgccag
     1201 aatattacta tgtgatcgcg cttgctggtc agtccaatgg tatgtcatac ggtgagggac
     1261 tgccattgcc ggatacattc gacagccctg atccacgtat taaacagtta gcgcgtcgca
     1321 gtacggtgac accgggcggt gcagtatgca aatataacga catcattccg gcggaccatt
     1381 gtctgcatga tgtgcaggac atgagccgtc ttaaccatcc gaaagcggac ctgtcaaagg
     1441 ggcagtacgg aaccgtgggg caggggctgc atatcgccaa aaaactgctg ccgtttatac
     1501 cggcgaatgc gggcattctg ctggttccgt gctgtcgtgg tggttcagcg ttcaccaccg
     1561 gagccgatgg cacatacagt gacgcgagtg gtgcctcgga gaattcaacc cgctggggtg
     1621 tggacaagcc gctgtataag gaccttatcg gtcgaacaaa agcagcactg aagaagaatc
     1681 cgaaaaatgt gctgtttgcc gtggtgtgga tgcaggggga atttgatttt ggcggtacgc
     1741 cggtaaatca cgccgcacag tttggtgcgc tggttgataa attccgtgca gacctggcgg
     1801 atatggcagg ccagtgcgtc ggtggctctg ctggcggtgt tccctggata tgcggggaca
     1861 cgacgtattt ctggaagcag aagaacgaat ccacgtacca gacggtgtat ggcagctata
     1921 aaaacaaaac ggaaaagaat atccatttcg taccgttcat gacggatgag aacggggtga
     1981 atgtgccgac gaacaaaccg gaagaagacc cggacattcc gggtatcgga tattacggtt
     2041 cgaaatggcg tgacagctca gccacctgga cgtcacagga cagggcgagc catttcagtt
     2101 catgggctcg ccgcgggatt atttccgacc gtctggcaac ggcgattttg cgccatgcgg
     2161 gaagagtggc gctaaacgcg ggggcatcat cgacagtatc agaggtgcgc ccgtcatcgc
     2221 cttccggtgc agaagccaca ggcgtcacaa cactgctctc ttaccttgcc agcgagtcag
     2281 agggaagcct gaaagtacag ggatggtcag ccagtggcgg cagggcagaa gtggtcagcg
     2341 atgcggaggg aaccggaggt aaggcagtga agctgaccaa ggaagccggt aaaagcagct
     2401 gggtgctgga gtacgccgcg ggcaacggtg cggctctgtt acagaaaggg gggcagattc
     2461 gctgccgctt taaggtttcg ggagcgctgg ctgcgaacca gtatgttatg gcgttttact
     2521 ggccggtatc ttcactgcca cagggcgttg ccctgaccgg agacgggggg aataacctgc
     2581 tggcagcgtt ctacatccag acagatgcaa aagacctgaa tgtgatgtac cacaatgcga
     2641 aagtggcgac aaacaacctg aaactgggaa cctttggcgc atttgataac gaatggcata
     2701 cgctggcttt ccgctttgcc gggaataaca gcctgcaggt gacgccggtt attgatggtc
     2761 aggatggcac accgttcacg ctgacgcagt caccggtcag tgcctttgcg gcggataaac
     2821 tgcatgtgac agacattacc agaggtgcga cttacccggt actgatagac agcattgcgg
     2881 tggaagtgaa cagcacagac actgcggcat gataaaaaaa ccgccagcga caggaatgga
     2941 cgctggcggt ggtgatacct atggagaaaa aataaaggaa cgatactttc gtactctggt
     3001 ttttaatgaa aacagttctt attgtcaaca ataacggaaa gaaattatga catttctgaa
     3061 ccagttaatg ctgtacttct gtacggtggt ctgtgtgctg tatctccttt cgggtgggta
     3121 cagggccatg cgtgacttct ggcgcagaca gattgacaaa agggccgctg agaaaatcag
     3181 cgccagtcag tcagccggaa gcaaacccga agagccgctc atttagcggc aactttctta
     3241 atcacacctt tcgacgagaa aatcccatgt cagaaattac atccctggtc actgcagagg
     3301 cagtgaagga cgtcctgcgc tctgaagaag tccggagcgc actgaaacag aaacttcgcc
     3361 ataacctgga agcgcgtctt gatgcagaag tggatgccat tctggatgaa ctgcttggtg
     3421 tacaggcaga gccaccgact gaagcgggag ataccaccgc agagagcggt gaagttcagc
     3481 ctgaatcacc ggtcgccgat gcgactgaac ctcaacccga atcggtcatg atgctgtaac
     3541 ggggagtcag ggccatcagt aaacagctgc tggccttttt catgttgtga gcttccggat
     3601 tgcgggagac ggggtatgta ccagatggaa aaaatcacaa caggtgtgtc atacaccacg
     3661 tcagcggtgg gaacgggcta ctggttcctg cagttgctgg acagggtttc cccgtctcag
     3721 tgggcggcaa taggcgtgct ggggagtctg ctgtttggtc tgctgacgta cctgacgaac
     3781 ctgtatttca agattaaaga agaccggcgt aaggcggcgc ggggagagta agctgatgag
     3841 caggaaattc cgctatggtt tatcagcggt agttctggcg ctgattgccg caggtgcttc
     3901 tgcgcctgaa atcctcgacc agtttctgga tgaaaaggaa ggtaaccaca ccacggcata
     3961 ccgtgatggt gtgggtatct ggaccatctg ccgtggagcc acccgggtgg atggtaagcc
     4021 tgtgattcct ggcatgaagc tgtcgaagga aaaatgcgac cgggttaacg ctatcgaacg
     4081 ggataaggcg ctggcatggg tggcgaagaa catcagagtg ccactgactg aaccccagaa
     4141 agcgggtatt gcgtcattct gtccttacaa cattggcccc ggtaagtgtt tcccgtcgac
     4201 gttttataaa cgaattaatg caggtgatcg aaaaggtgcc tgcgaagcga ttcgctggtg
     4261 gattaaggac ggtggcagag actgccgtat ccgctcaaat aattgctacg gtcaggtatc
     4321 aagacgtgac caggagagtg cgctgtcatg ctggggtatc gacagataag cagaatattt
     4381 tgctgaaaaa tgacgttggc caacgcggca gataacacga aatcctgcaa actggcaaaa
     4441 tgtaagtgaa taaagttaac aagattgttt catgaagagg caccgtaatg gtgcctttgt
     4501 catttctgcg cttcgcacaa gcgtaaataa accaaagaac ctttcaggat gagccctggt
     4561 ggataaccgg cagtggtctg gttaaccctc tttgggctgg ttattcctgt gcgcagggtt
     4621 catcactaaa aggaaataac cgatgaatat gatgaccgtg ccgtttcacg gcgattctct
     4681 ttatgtggtt aaccataacg gcgaaccata cgttcccatg aaacctgtcg ttgcggggat
     4741 ggggctggcc tggcaatcac agttggctaa gttaagacag cgttttgcgt caactataac
     4801 ggaaatcgtt atggttgctg aggatgggaa acgacgcaat atggtgtccc tgccgcttcg
     4861 aaaacttgca ggctggttac aaaccatcaa tcccaacaaa gtaaaacccg aaatccgcgg
     4921 caaggtaatc cagtatcagg aagagtgtga cgatgttctc tatgaatact ggacgaaggg
     4981 ttttgtcgtt aatccccgtc gaatgagtgt gatggaagaa ctcaatcagg cttgcgctga
     5041 catgaaacgg gataaaaaca ttgccagtgt gtttgctacc gggctgaatg agtggaaaca
     5101 ggttaaatcc gcgcatgtat caaaaatccg cacattgata aacgaagcga atctgctgat
     5161 tgattttgtc ctggctgata caggcaaagg gaaaataaca aaggcggatt gatggagtgg
     5221 tggctaatga tatcggataa actcataacg ctggcgaaga tcctctgtgt aatcgtcggc
     5281 atttcatttt tagtcatgct ggttgccatt ttcttttcca ccgcctggcg agtcctgacg
     5341 ttatcgggac tggtggggtg aaagagagat gaaccgtgtt ctgtgtgtgg tgattattgt
     5401 cctgctggta gcctgtggtg cgcttagtct ggggctgaat cattaccgcg ataacgccat
     5461 aacctacaaa gagcagcgcg ataaaaaagt cagtgagctg gagctggcaa atgcaaccat
     5521 tactgatatg cagcagcgcc agcgtgatgt tgctgcactt gatgccagat actcgaggga
     5581 attagccgat gcgagagctg aaaatgaaac tcttcgcgct gacgttgccg ctggttaccg
     5641 cagcctgcgg atcaacgcca cctgtccagg tcccgtgcgt gaagccaccg gcaccgcccg
     5701 cgtggataat gcaaccggcc cccgactggc agacaccgct gaacgggatt atttcaccct
     5761 cagagagagg ctgatcacta tgcaaaaaca actggaagga acccagaagt atattaatga
     5821 gcagtgcaga tagagttgcc catatcgatg ggcaactcat gcaattattg tgagcaatac
     5881 acccgcgctt ccagcggagt ataaatgcct aaagtaataa aaccgagcaa tccatttacg
     5941 aatgtttgct gggtttctgt tttaacaaca ttttctgtgc cgccacaaat tttggctgca
     6001 tcgacagttt tcttctgccc aattccagaa acgaagaaat gatgggtgat ggtttccttt
     6061 ggtgctactg ctgtctgttt gttttgaaca gtaaacgtct gttgagcaca tcctgtaata
     6121 agcagggcca gcgcagtagc gagtagcatt tttttcatgg tgttattccc gatgcttttt
     6181 gaagttcgca gaatcgtatg tgtagaaaat taaacaaacc ctaaacaatg agttgaaatt
     6241 tcatattgtt aatatttatt aatgtatgtc aggtgcgatg aatcgtcatt gtattcccgg
     6301 attaactatg tccacagccc tgacggggaa cttctctgcg ggagtgtgcg gggataatca
     6361 aaaacgatgc acaccgggtt ttctcatttt tcacgagatg ggagcgattt cccgcgaagc
     6421 cgcctgtccg gtgcggtggt ggaagaaacc ggataaaaca accgcattgt gcaaatatcg
     6481 atcaaatatg gtgctgctgt gtgaaatctg aaaaatcaca gcggtcatta tgcatcagtt
     6541 tttaacacag gacgtcagaa cgtgacatgg caaagctgga ctggaaaaag ctggagcagg
     6601 cattccgacg cgaacatgcc gaaacgggaa taacattact ggactggtgc cggaagaaaa
     6661 agattaatta caacaccgcc agaacccgta taaaaatggg caaaatcgat catgaaattg
     6721 atcataaaac cgatcatgaa atcgatcatg acatctcaga tgaagaaccc tgcaatgacg
     6781 cgggttccgg cgatgaaaaa tgcgcaaaaa actctgaaaa aaactgcgca aattcggcag
     6841 aaacgaaacg gattcgtggt tcccgacttt taccaccttc aaacgctttt tctcagcgaa
     6901 acacccacgc cgtaagacac cgtggatatg cgaagtatct tgaggcagat aacctcatgg
     6961 atgatgcgtc cgacatggtg ctgttcgatg aactggtgtt cacccgggcc cgcgcacttt
     7021 cagtaactaa ggcacttaaa gggatgttcg ccgacctgga agaggcaact gacgtggaaa
     7081 cccgtgttgc tctttacgac aaaatactca aagctgaaca ggcccttgac cggaatattg
     7141 cccgtatcga gtcaattgaa cgctcattgc tgacgctgga cgtcctggct gagacagcac
     7201 caaaacttcg tgctgaccgg gaaagaatca acgccgccag agataaactc agagctgaaa
     7261 ccgatattct gaccaaccag cgtcggggcg ttgttacgcc tgtcagtgac atcgtgtcat
     7321 cgctgcatga aatgagtaat tcggggagac tggatgacat tccggaagaa tgaaccgcga
     7381 tgtgatgagc cgtcagaaat gaccgaggct gaacaacgtc tgttcatcat gactaaactg
     7441 agcaatccct ggtggcggct caatcatctc tacaaaatac agaacgaaaa aggtgaactg
     7501 gtcaccttca gaatgcgacc ggcgcagcgc cagttgttcc ggagcatgca caataaaaat
     7561 attatcctga aagcgcgcca gctgggattt tccacagcca ttgatattta tcttctcgac
     7621 caggcattat tcattccgca tctcaaatgc gggatcgtcg ctcaggataa acaggctgcc
     7681 agtgaaattt tccgcacaaa aattgctgta ccgtttgatc atctccctga ctggctgaga
     7741 gcctcattca ccatcgttga acgtcgtagc ggtgccagcg gtggctatat cctgtttggt
     7801 cacggctcga gtatccaggt ggcaacctca ttccgttcag gtacggtgca gcgcctgcat
     7861 atctcagagc acggcaaaat ttgcgcgaaa tatccggcta aggcgaaaga actgcgaacc
     7921 ggtacgctta atgccgtctc tgatgaatgc attatttttg atgagtccac tgctgaaggc
     7981 gtgggtggtg atttttacga gatgagtaac cgagcacagg agatcactgc atcaggctta
     8041 ttgctgacgg cacaggatta taaattccat ttttacgcct ggtggcagga tcctaaatac
     8101 agcgccagag tgccggaaag cgggctgaag ctgtcacggg aaaaaatgac gtatttttct
     8161 gcggttgaga aggcaatgaa catcacgctt actgatgaac agaagcagtg gtacatcaat
     8221 aaggaaactg aacagcgtga ggaaatgaag caggagtttc cctcaacgcc acaggaggcg
     8281 tttctgacgt ccggacgacg tgtgttcagt gccgaaagta cgttgcaggc agaatcattc
     8341 tgttcgccac cgatgattgt ttatgacatt gaacctgtta caggagcgaa gactaaagct
     8401 cagtctctgc gtgaaggaaa taaaaacgag ttgcagcgga cgctgatgaa ttatctgctg
     8461 gtatgggaac tgccggatcc ggatgaagag tatgtttgtg gggcagatac tgccgaaggg
     8521 ctggagcacg gagaccgctc atcgctggat gttgtcaaac gcagtaatgg cgagcaggtg
     8581 gctcactggt tcgggcatct cgatgctgaa ctttttgctc atctcatttc gcaggtctgt
     8641 cgtatgtata acaacgcgtt tgtggggccg gagcgtaata atcacggaca tgcagttatc
     8701 ctgaaactcc gggaactcta tccgacacgt tatatctaca acgaacagca tcttgaccag
     8761 gcatatgacg acgatacgcc ccgccttggc tggctgacaa cccgtcagag caaacctgtt
     8821 ctgaccgaag gaatgaaaac gcttctgaat aatggaatat cagggatccg ctggtcaggc
     8881 acattatcgg aaatgaacac ctacgtttat gacgcgaaag gctccatgaa tgcacaggaa
     8941 ggctgctttg atgatcagct catgagctac atgattgccc aggagatgcg cgccagaatg
     9001 ccggtgaggg taaaacagaa aacggataaa cgcagaacca cacactggat ggcacactga
     9061 tgaaaaatga aattaacacc acagcgatga aaaacgatca tggatccacg ccgcgttttt
     9121 ctcagcgtca gttactgtct ctctgttctg atattgacag tcagcctctc tggcgtgatg
     9181 ccgcaaacaa ggcctgtgcg tattatgatg gcgaccagct ggcaccggaa gttatccagg
     9241 tactgaaaga tcgcggtcag cccatgacca tccataacct cattgccccc acggtagatg
     9301 gtgtactggg aatggaggca aaaacaagaa cggacctgat agtgatgtca gacgatccga
     9361 acgatgatac agagaaactg gcagaggcca ttaatgcgga gtttgctgat gcgtgccgtc
     9421 ttggcaacat gaataaggcc cgctccgatg cctatgcgga acaaatcaag gcgggcctca
     9481 gttgggtgga ggtcagacga aacagtgatc cgttcgggcc tgaatttaag gtgtctactg
     9541 tcagccggaa tgaggttttc tgggactggc tgagccggga ggctgattta agtgactgcc
     9601 gctggctgat gcgtcgccgc tggatggata ccgatgaggc aaaagctaca ttcccgggaa
     9661 tggctcaggt tatcgattat gccattgatg actggcgtgg ttttgtcgat accacggtta
     9721 ctgaaggcca gcccagtccg ttgatgagtg catgggaaga gtatcagtca tgggatcgac
     9781 agcagaacga atggcttcag cgtgaacgcc gtcgtgtgct gcttcaggtg gtttattacc
     9841 gtacattcga gcgtcttccg gtgattgaac tcagtaatgg acgggtggtg gcctttgata
     9901 aaaataatct gatgcaggcg gtagctgtgg catccgggcg ggttcaggtg aaagtcgggc
     9961 gggtaagccg tattcgtgaa gcctggtttg tcgggccgca ctttattgtg gatcgcccct
    10021 gtagtgctcc gcaggggatg tttccgctgg ttcctttctg gggataccga aaggataaaa
    10081 ccggggagcc atacgggcta atttcccgcg ccattccggc acaggatgag gtgaattttc
    10141 gtcgtatcaa gctgacctgg ttgcttcagg ccaaacgtgt gattatggac gaggatgcca
    10201 cccagttgtc agacaacgac ctgatggagc agctcgaacg tccggatggc attattaaac
    10261 tgaatccggt ccgaaaaaat cagaaaagtg tcgcagatgt ttttcgggtt gagcaggatt
    10321 ttcaggttgc cagccagcag tttcaggtca tgcaggaatc ggaaaaactt atccaggata
    10381 ccatgggagt gtattccgca tttctcgggc aggattcagg tgcgacgtca ggcgtggcta
    10441 tcagtaacct ggtggagcag ggggccacaa cccttgcgga aatcaacgat aactaccagt
    10501 ttgcctgcca gcaggtggga agactgttgc tggcttatct tctcgatgac ctgaaaaaac
    10561 gccgtaatca tgcagtggtg attaatcgcg atgatcgcca gcgtcgccag accattgtcc
    10621 tcaatgctga aggtgataat ggtgaactga ccaatgatat ttcaaggtta aatacacata
    10681 ttgcgctggc gcctgttcag cagacaccgg cgtttaaggc acagcttgca cagagaatgt
    10741 cagaggttat tcaggggctg ccgcctcagg tgcaggctgt tgtgctcgac ctgtgggtta
    10801 atcttctgga tgtgccgcag aaacaggagt ttgttgagcg tattcgtgct gcgctgggga
    10861 cgccaaaatc accggatgaa atgacgccgg aagaacagga agtagcggca caacaacagg
    10921 cacttcagca acaacaggca gaactccaga tgcgcgagat ggctggcaga gtggcaaaac
    10981 tggaagctga cgccgccagg gcacatgcag ctgcacaacg ggataatgcc agtgcacagc
    11041 gggaagtcgc cctgacacag gggcagcgtt atgtggatgc gcttaaccag gcacatacgg
    11101 cagaaatcat taccggcgta cagaatatgg aacaggagca ggacgttctt cagcaacaga
    11161 tgctgtatac gttacaacag cggatgaatg aaatgtcgct ctgaaaactc tggcttcaac
    11221 tgaaccccgt catcgtacgg ggttttttgt ttccggaggt aagcgttccg ggagcggtgc
    11281 gcttattcgc gggggcagcg ataagcctta tttactcaac cattcggatc tgtccgataa
    11341 acagaccatg cggagttatt tatggatttt gaatttacgg gtgaagaaac cccggaacaa
    11401 ctggaaaaaa tgctggaagg acttggggat gtggatattg acagtcacgc acaggacgtc
    11461 gtgacggaag ataccacgga aaaacatgcg gatgaggaag cacagactca gacgggcgat
    11521 aacaatgtgg caccgacgcc ggatgccagt gtggagcaga cgcaggacgt gaaggagccg
    11581 gaagcgaagg gggtgctcac ccgcgacggt aaacacgtca ttccctatga agtccttgag
    11641 gctgaacgtt ccggtaagca acgggccgaa caggaagccg cacttcttcg tgggcagata
    11701 gctgaagaaa aacgcagggt ggaactgctg acgtctcaga tccaccaggc cggtatgaag
    11761 cccacaccgt taccggaaaa cgaaaaaatt tctgatgagc agattgcccg tatcagggag
    11821 atgtatccgg aaattggtga cgcggtggct tcgctcatcc gtaaaaataa ctatctc
//
LOCUS       DBFIFM010000138        11753 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.138, whole genome shotgun sequence.
ACCESSION   DBFIFM010000138 DBFIFM010000000
VERSION     DBFIFM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.138"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            89..385
                     /locus_tag="N7C74_003312"
     CDS             89..385
                     /locus_tag="N7C74_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HGU2433126.1"
                     /translation="MNAYYIQDRLEAQSWERHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            391..1176
                     /gene="bet"
                     /locus_tag="N7C74_003313"
     CDS             391..1176
                     /gene="bet"
                     /locus_tag="N7C74_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HGU2433127.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKASEQKVAA"
     gene            1173..1853
                     /locus_tag="N7C74_003314"
     CDS             1173..1853
                     /locus_tag="N7C74_003314"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HGU2433128.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARVLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYLAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            1850..2032
                     /locus_tag="N7C74_003315"
     CDS             1850..2032
                     /locus_tag="N7C74_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HGU2433129.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIQNPLKAQRLAEE
                     INNKRGAVCTKHLLLS"
     gene            2005..2196
                     /locus_tag="N7C74_003316"
     CDS             2005..2196
                     /locus_tag="N7C74_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HGU2433130.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLAASL
                     SQKLEMMVAKAEADERDQV"
     gene            2273..2488
                     /locus_tag="N7C74_003317"
     CDS             2273..2488
                     /locus_tag="N7C74_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGU2433131.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            2587..2808
                     /locus_tag="N7C74_003318"
     CDS             2587..2808
                     /locus_tag="N7C74_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HGU2433132.1"
                     /translation="MADLIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            2805..3752
                     /locus_tag="N7C74_003319"
     CDS             2805..3752
                     /locus_tag="N7C74_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU2433133.1"
                     /translation="MSEINYQALREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDAQAYANAEFIAAFNPNVALALLDERERNQQYIKRRDQEN
                     EDIALTVGKLRVELEAAKSKLNEQREYYEGVISDGSKHIAELEKQCAEWERKALSNFE
                     ECAAMAERIEEMQTKSAPDSFGIIGENIRTQDNRITSDPMFCVYQKREIVVDADYDYD
                     RIVWVDEDGNEANKLQSRRLELLHENFREPPEKWRRVAVKDIDEFVTCCFTEQGCKDY
                     LAVNGHNLRLPFIYVKSGFRNAEYIGIRNWLAGIRIKGE"
     gene            3754..4200
                     /locus_tag="N7C74_003320"
                     /pseudo
     CDS             3754..4200
                     /locus_tag="N7C74_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749537.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4220..4435
                     /locus_tag="N7C74_003321"
     CDS             4220..4435
                     /locus_tag="N7C74_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433134.1"
                     /translation="MAIAASYTMHLYCDCRQCTNGKYQTPDFGEYIGTSWAGCAKEAR
                     KDGWRINKDKTRAFAPGHKILRSNKGE"
     gene            4437..4655
                     /locus_tag="N7C74_003322"
     CDS             4437..4655
                     /locus_tag="N7C74_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HGU2433135.1"
                     /translation="MPTLFRKEYPRKSRATEFLFLILFIVLMTPISPLIFVWAIGKII
                     ELVIELYNDVVWASFNTLHNKINPYKEN"
     gene            4657..4944
                     /locus_tag="N7C74_003323"
     CDS             4657..4944
                     /locus_tag="N7C74_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433136.1"
                     /translation="MATLQELIDLTPEQEKAWNRLVKAVKDFRAAGGKFYSVLDTLSA
                     YNGEHVASIDNDKGYHTASVYMPSIDAPGLTSWADDWHGITLKDGVEVDKD"
     gene            4948..5079
                     /locus_tag="N7C74_003324"
                     /pseudo
     CDS             4948..5079
                     /locus_tag="N7C74_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5092..5565
                     /locus_tag="N7C74_003325"
     CDS             5092..5565
                     /locus_tag="N7C74_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001451275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU2433137.1"
                     /translation="MGIELVKYGCNTFICPDNSMEPGNVPLYIGLPRIDPASQTAKLS
                     FQEWLSEQKEKIDVDCGCVSIETLTHWMKSAYEAGNSPVTPDSWISCSDRMPEKGQNV
                     LISVNFDSSLVEPLICSARYTGSTFRRGDATIKPGNGIEQATHWMPLPEPPQEVN"
     gene            5565..5849
                     /locus_tag="N7C74_003326"
     CDS             5565..5849
                     /locus_tag="N7C74_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HGU2433138.1"
                     /translation="MDSFAKYTIIDWIAFLQVLLIWFYLAYRSGQWIVSVACSKGWRW
                     WNRKNKKALALDSFYEAFNLNSLQPGSIIVVTTQSDMTIQIHKPKEEGRG"
     gene            6205..6828
                     /locus_tag="N7C74_003327"
     CDS             6205..6828
                     /locus_tag="N7C74_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HGU2433139.1"
                     /translation="MTSQLIPVFNGTIDNETILLVNARDLHSFLGVGRMFAHWVKERI
                     AEYGFVESQDYILICQNGQTKGRGGDRRSKDYHLTLDTAKELAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMEKAQDISKRLYPILKELNSSYASKLYD
                     IAFETFYTVTKNRDALLREATRIDQTSAIFERARPMLKSLRARQFEF"
     gene            6871..7038
                     /locus_tag="N7C74_003328"
     CDS             6871..7038
                     /locus_tag="N7C74_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433140.1"
                     /translation="MKFKFTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHAEVTNI
                     RIEELKEHQAA"
     gene            7265..7891
                     /locus_tag="N7C74_003329"
     CDS             7265..7891
                     /locus_tag="N7C74_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HGU2433141.1"
                     /translation="MTKKYTLIYADPPWTFRDKATDGQRGASFKYPVMSLLDICRLPV
                     WELAADNCLLAMWWVPTQPLEALKVVEAWGFRLVTMKGLTWNKCGKRQTDKLVMGMGS
                     TTRANSEDCLFAVKGNLPERINAGIIQSFTAPRLDHSRKPDMAREKLVQLLGDVPRIE
                     LFARHTSHGFDVWGNQCGTPSIEMVPGIVKFLEKTNERKNDVDKGITS"
     gene            7851..8063
                     /locus_tag="N7C74_003330"
     CDS             7851..8063
                     /locus_tag="N7C74_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HGU2433142.1"
                     /translation="MSEKTTLTKASPVELRQCLEIANQLARSGIRFVPIPITADTELH
                     LFGEILSRKLDELEKLVEEADTSPIA"
     gene            8099..8476
                     /locus_tag="N7C74_003331"
     CDS             8099..8476
                     /locus_tag="N7C74_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU2433143.1"
                     /translation="MNAVLTELNKLGKATADSISKGLNIDLNDVIDTLWKLKNQGIVK
                     VKNGIWQAVEGVGSKTDIDSVQPVQPVQPVQPVQNNIIGDLLRKSRKEARRAGRKQKR
                     WEGACKALQELNKYRDLINELSE"
     gene            8555..8737
                     /locus_tag="N7C74_003332"
     CDS             8555..8737
                     /locus_tag="N7C74_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2433144.1"
                     /translation="MEVNLMTEKEVSDLLQKRRTALYNLRKKHGFPEPVLTHPARYSR
                     QAVEQWLKAGGINRAV"
     gene            complement(8721..9890)
                     /locus_tag="N7C74_003333"
     CDS             complement(8721..9890)
                     /locus_tag="N7C74_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2433145.1"
                     /translation="MALSDTKLRGLYGKPYSGPAEITDGDGLSVRITPAGTITFQYRY
                     RWNGKPVRLTVGRYPSTSLKDARVIVGEMRALYMKGVNPKNYFAPSDGELTLKECLDQ
                     WWDKYVTDLKPNTQTLYRSVVYNTMYTQFEGSPVASIPVSAWARFFDKQESLNKKKAR
                     VLLLQLRSVINWCISRQLIPSCELLKLSVKNIGKKPDVGSRVLTYTELAKIWLALENS
                     KVVTSNKVLHQLLLLWGARLSELRLATASEFNMEDLVWTTPKEHSKMGNIIRRPVFTQ
                     VKPYIERLLNAGFDVLFPGQEIDKPIDRSSANLYMKKLREKIDIPEWRTHDFRRSLVT
                     NLSGEGIMPHVTEKMLGHELGGVMAVYNKHDWLSEQKDAYELYADKIFWHAKQLG"
     gene            10086..10160
                     /locus_tag="N7C74_003334"
     tRNA            10086..10160
                     /locus_tag="N7C74_003334"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10119..10121,aa:Arg,seq:cct)
     gene            10322..11479
                     /gene="intS"
                     /locus_tag="N7C74_003335"
     CDS             10322..11479
                     /gene="intS"
                     /locus_tag="N7C74_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HGU2433146.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKRQVWSAAYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLAEAMKGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMQWKNVDFENRIITIEASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKSICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDGKVE"
     gene            complement(11654..>11753)
                     /locus_tag="N7C74_003336"
     CDS             complement(11654..>11753)
                     /locus_tag="N7C74_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433147.1"
                     /translation="EKPINNKLRKWWSGFKNDVSTTRVEKIEGNQL"
BASE COUNT         3463 a         2638 c         2840 g         2812 t
ORIGIN      
        1 aagcattcac tacccccctt tcctgttttc ctaatcagcc tggcatttcg cgggcgatat
       61 tttcacagcc attttcagga gttcagccat gaacgcttat tacattcagg atcgtcttga
      121 ggctcagagc tgggagcgtc actaccagca gatcgcccgt gaagagaaag aggcagaact
      181 ggcagacgac atggaaaaag gcctgcccca gcacctgttt gaatcgctat gcatcgatca
      241 tttgcaacgc cacggggcca gcaaaaaagc cattacccgt gcgtttgatg acgatgttga
      301 gtttcaggag cgcatggcag aacacatccg gtacatggtt gaaaccattg ctcaccacca
      361 ggttgatatt gattcagagg tataaaacga atgagtacag cactcgcaac gctggcaggg
      421 aagctggctg aacgtgtcgg catggattct gtcgacccac aggaactgat caccactctt
      481 cgccagacgg catttaaagg tgatgccagc gatgcgcagt tcatcgcatt gctgatcgtc
      541 gccaaccagt acggtcttaa tccgtggacg aaagaaattt acgcctttcc tgataagcag
      601 aacggcatcg ttccggtggt gggcgttgat ggctggtccc gtatcatcaa tgaaaaccag
      661 cagtttgatg gcatggactt tgagcaggac aatgaatcct gcacatgccg gatttaccgc
      721 aaagaccgca atcatccgat ctgcgttacc gagtggatgg atgaatgccg ccgcgaacca
      781 ttcaaaaccc gcgaaggcag agaaatcacc ggaccgtggc agtcgcatcc caaacggatg
      841 ttacggcata aagccatgat tcagtgtgcc cgtctggcct tcggatttgc tggtatctat
      901 gacaaggatg aagccgagcg cattgtcgaa aataccgcat acactgcaga acgtcagccg
      961 gaacgcgaca tcactccggt taacgatgaa accatgcagg agattaacac tctgctgatt
     1021 gccctggaca aaacatggga tgacgactta ttgccgctct gttcccagat atttcgccgc
     1081 gacattcgcg catcgtcaga actgacacag gccgaagcag tgaaagctct tggattcctg
     1141 aaacagaaag cctctgaaca gaaggtggct gcatgacacc ggacattatc ctgcagcgta
     1201 ccgggatcga cgtgagagct gtcgaacagg gggatgatgc gtggcacaaa ttacggctcg
     1261 gcgtcattac agcttcagaa gttcacaacg tgatagcaaa accccgttca ggaaagaaat
     1321 ggcctgacat gaaaatgtcc tacttccaca ccctgcttgc tgaggtttgc accggtgtgg
     1381 ctccggaagt taatgccaaa gcactggcct ggggaaaaca atacgagaac gacgccagag
     1441 tcctgtttga gttcacctcc ggcgtgaatg ttactgaatc cccgattatc tatcgcgacg
     1501 aaagtatgcg tacagcctgc tctcccgatg gtttatgcag tgacggcaac ggccttgaac
     1561 tgaaatgccc gtttacctcc cgggatttca tgaaattccg gctcggtggt ttcgaggcca
     1621 taaaatcggc ttacctggcc caggtgcagt acagcatgtg ggtgacgcga aaagatgcct
     1681 ggtactttgc caactatgac ccgcgtatga agcgtgaagg actgcattat gtcgtggttg
     1741 agcgggatga aaagtacatg gcgagttttg acgagatggt gccggagttc atcgaaaaaa
     1801 tggacgaggc actggctgaa attggttttg tatttgggga gcaatggcga tgaagcatcc
     1861 tcacgataat atccgcgtag gcacgatcac tttcgtctac tccgttacaa agcgaggctg
     1921 ggtatttccc ggcctttctg ttatccaaaa tccactgaaa gcccagcggc tggctgagga
     1981 gataaataat aaacgagggg ctgtatgcac aaagcatctc ctgttgagtt aagaacgagc
     2041 attgagatgg cacatagcct tgctcaaatt ggagtcaggt ttgtgccaat accagtagaa
     2101 acagacgaag aatttcatac gttagccgca tccctttcac aaaagctgga aatgatggtg
     2161 gcgaaagcag aagcagatga gagagaccag gtatgacaac cactgaatgc attcttctgg
     2221 cagcgggctt tatattctgt gtgcttatgc ttgccgacat gggacttgtt caatgacacc
     2281 tcagcaggaa aacgcccttc gcagcattgc ccgtcaggct aattctgaaa tcaaaaaagc
     2341 cagacagcag tttccggata aaaacgtcga tgacatttgc cgtagcgtac tgaagaagca
     2401 ccgcgaaacg gtaacgctga tgggattcac accgactcac ttaagtctgg caatcggtat
     2461 gttaaacggc gtctttaagg aacggtgaac atgaaaagca aaatcatcag ggagctacag
     2521 gctccttttt tattattcgc attcaccctc aagcgtatta accaacaatt cagggattaa
     2581 tgaaagatgg cagacctcat tgattcagca tcagaaattg aagaattaca gcgcaacacc
     2641 gcaataaaaa tgcgccgcct gaaccaccag gctatatctg ccactcattg ttgtgagtgt
     2701 ggcgatccca tagatgagcg aagacgcctg gccgttcagg gttgtcggac ttgtgcaagt
     2761 tgccaggagg atctggagct tatcagtaaa cagagaggtt cgaagtgagc gaaattaatt
     2821 atcaggcact gcgtgaaaag gcagagaaag caactaaagg aagctacatc gtagggcata
     2881 catctgttaa ccaacacggc aatttaacag gagtttttgt ttgccaaaaa tggaaaggag
     2941 aacccggtgg cgtgattgca gaatgtcatg ttaactgcct ggttgaaaca gatgctcagg
     3001 cttacgcaaa cgctgaattt attgctgcct ttaatccaaa tgttgcgctg gcactgctgg
     3061 atgaacggga aagaaaccag caatacatca aacgccgcga ccaggagaac gaggatattg
     3121 cgctaacggt agggaagctg cgcgttgagc ttgaagcagc aaaatcaaaa ctcaacgagc
     3181 agcgtgagta ttacgagggc gttatctcgg atggaagtaa gcacatagca gaactggaaa
     3241 aacaatgcgc cgaatgggag cgaaaagcat taagcaactt tgaagagtgt gctgcgatgg
     3301 ctgaacgtat cgaagagatg cagacaaaat ctgcaccaga ttcgtttggc atcatcggtg
     3361 aaaatattcg aacacaggat aatcgaataa cgtcagatcc tatgttttgt gtgtatcaaa
     3421 agcgcgaaat cgttgttgat gctgattatg actatgaccg gattgtctgg gttgatgaag
     3481 atggcaatga agccaataaa ctccaaagtc gtcgtctcga actacttcat gaaaactttc
     3541 gggagccacc agaaaaatgg cggcgcgttg ctgtgaaaga tattgatgaa ttcgttacct
     3601 gctgtttcac cgaacagggt tgtaaagatt acctagcagt caatggtcac aatcttcgat
     3661 tgccatttat atatgtaaaa agcggtttca ggaacgctga atatatcggc ataagaaact
     3721 ggcttgctgg cattcgcatc aaaggagagt gatatgacca ctataaccga taagaaacag
     3781 tatcccagcg agcaatttct taatgagctg atcaccaaca tggagtttgc tgcaagggca
     3841 ccagttgaag tcgtgagagc gatggcagca gagctacaga agcggcgcga agctgatagt
     3901 gcagaaccag ttgcttatat tttcaaacat ccggccggga aattattctg ggctttaacg
     3961 gatgaaagca ataaagagca agcggacgtt attcctgttt atgctgccgc gcctgcgtcg
     4021 gttgtgccgg ataatgcatc agagcctctt gcttatgctt acaaagagct tacgcctgag
     4081 attatgcgca accatttagc tgtattcgag cgatatggaa tagccccaaa cgatagcact
     4141 accacaattc aggcactgcg aatcgcgctg gatggtatag agcggagcga cgccatgctt
     4201 cagggtaaag gagggggata tggctattgc cgcaagttac accatgcatc tctactgtga
     4261 ttgtcgccag tgtacaaatg gtaaatatca aacgccagat ttcggtgagt atatcggtac
     4321 gtcatgggct ggctgtgcaa aagaggcgcg caaggatggc tggcgaataa acaaagacaa
     4381 aacgcgtgct tttgcgccag ggcataaaat tttgaggagc aacaaaggag agtgatgtgc
     4441 ctacattatt cagaaaagaa tatccgcgaa aaagtagagc gacagaattt ttgtttctca
     4501 ttctgtttat cgtgttgatg acaccgatat ccccgctaat ttttgtctgg gcaatcggga
     4561 aaataattga gctagttatt gagttgtata acgacgtggt atgggcgtcg ttcaacacac
     4621 tgcacaataa aattaaccca tataaggaaa actgaaatgg caactttgca ggaattaatc
     4681 gacctgacgc cagaacagga aaaagcgtgg aatcgtcttg tgaaggctgt aaaggatttc
     4741 agggcagccg gaggaaagtt ttatagtgtc ctagacacgc tgagcgcata caacggcgag
     4801 cacgttgcca gcattgataa cgataagggc taccacactg caagcgtcta tatgcctagc
     4861 attgatgcgc ctgggttaac cagttgggct gatgattggc acggcatcac gctgaaagat
     4921 ggcgttgaag tggataagga ctaacacatg acaacgttca ccgacaaaga aatgattaaa
     4981 gaaatcaaag agcgaatcgg cagcctggac gtgcgagata atattgagcg ccgtgcttat
     5041 gaaattgcgt tgactgcatt gaccactgaa ccattcgcca ctatcgacac agtgggaatt
     5101 gagctcgtta aatatggttg taacacgttt atttgtcccg acaattcgat ggagccggga
     5161 aatgtaccac tatatatcgg cctgccacga attgatccag caagccagac tgccaagctg
     5221 tcattccagg aatggttgtc agaacaaaaa gaaaaaatag acgttgattg cggatgtgta
     5281 agcatcgaaa cgcttacgca ctggatgaaa tccgcttatg aggctggtaa ctctccggta
     5341 actccggata gttggataag ctgtagtgat cgaatgcctg aaaagggcca gaacgtgctt
     5401 atttcggtga atttcgatag ctctctggtt gaaccgctaa tatgctccgc acgctatacc
     5461 ggaagcacct ttcggcgcgg agatgcaacg attaagccgg gtaatggtat tgagcaagca
     5521 actcactgga tgccgctacc agaaccgccg caggaggtta attgatggac tccttcgcga
     5581 aatatacgat tattgactgg atagcattcc ttcaggtttt gctcatctgg ttttatttgg
     5641 cttataggag tggacagtgg attgtcagtg tagcctgtag caagggatgg cgttggtgga
     5701 accgaaagaa taaaaaagcg ctggccttgg attcgtttta cgaagcattc aatcttaaca
     5761 gccttcagcc tggttctatc attgtagtca ccactcaaag cgacatgacg atacaaattc
     5821 acaagccaaa ggaggaaggt cgtggctaac ctgcaacttg ccgttaaagg tgaatacttc
     5881 gcagatagtt tcccccaaat ctggggaaaa gcccgaatgg cgcggcttac agcaagataa
     5941 ggcctacatg atttgacaaa tccgccagag ctatcgcata ctgaccgcac tagaacttca
     6001 aagcggtcat ccgcacccga tagctttgcg gcttttttat gcctgcaatt tggcatagtc
     6061 acatccgtac aaaggtcggg tggagaggcg taatacaata cccgcaaggg gaatatgccc
     6121 ggagcatctt tgaaggctct agttgacacc cgatcaccag ccactaactg gtgattgcta
     6181 actaaaactt caaaggaggt catcatgacc agtcaactca tccccgtatt caacggcact
     6241 atcgacaacg aaacaattct tctagttaac gctcgtgatc tacactcttt tcttggtgtg
     6301 ggcagaatgt tcgcgcactg ggttaaagaa cgcattgctg aatatggatt cgttgaaagc
     6361 caggactata ttttgatttg ccaaaatggg caaaccaaag ggcgaggagg tgatcgccgt
     6421 agcaaggact accacctaac tctcgacaca gccaaagaac tggcgatggt tgagcgtaac
     6481 gaaaaaggcc gccagatacg ccgatacttc atcgagtgcg aaaagaaact tcgcagcatg
     6541 caaccagcac agcaattcac agacgaggaa atcatcctcc tctgctacat gcaggtacag
     6601 atggagaaag cacaggacat cagcaaacgc ctgtatccga tattgaagga actgaactca
     6661 tcatacgcga gcaagctgta tgacatcgcg tttgagactt tctacacggt gacgaaaaac
     6721 agagacgcat tgctcaggga ggcaacacga attgaccaga caagcgccat tttcgaacgg
     6781 gcaagaccaa tgttaaaaag ccttcgggcg agacaattcg aattttaata tcaaaggagc
     6841 ttcggctcct ttttttacag gtgaaactta atgaaattca aattcacagg tgaatggaat
     6901 ggagaaccat tcaacagagt tatcgaagcg gagaatatca atgactgcta tgaccactgg
     6961 atgatatggg cgcagatagc acatgcagaa gtaaccaata ttcgaattga agaactgaaa
     7021 gaacaccaag ccgcctgatg gcggtttttt attggagaca agaaatgtca gatttggcta
     7081 tgaaggtttt gaaatggcaa tcgactgact atgttggcat cagtagcgca actcttgcct
     7141 caatcgcatg tggactgaaa aagaatatct atggtcatag cttcggtgct ccacatgacg
     7201 cagccgattt cagacgatgc gttgcacttg ttgcacttta cattgatgag tttgaaaacg
     7261 cgctatgacg aaaaaataca ctctcattta cgcagatccc ccctggacat tccgagacaa
     7321 agcaaccgat ggtcaacgcg gtgcaagttt taaatatccg gtcatgagtc ttctggatat
     7381 ctgccgcctc ccggtatggg aactggcagc cgataattgc ctgttggcta tgtggtgggt
     7441 gcctacacaa ccacttgaag cattgaaggt tgtagaagcg tggggctttc gtctggtgac
     7501 gatgaaagga ttaacctgga acaaatgcgg gaaaagacag accgacaagc tggtcatggg
     7561 tatgggtagc accactcgcg ctaacagcga agactgcctt tttgcagtga aaggaaatct
     7621 gcctgaacgc attaacgccg gaataatcca gtcattcact gcaccgcgcc ttgatcactc
     7681 ccgcaagccg gatatggcgc gagaaaaact tgtgcaactt cttggcgatg ttccccggat
     7741 agaactgttc gcccgtcaca cctcgcatgg atttgatgtc tggggtaacc aatgcggaac
     7801 accatccatt gagatggttc cgggtattgt taaatttctg gagaaaacca atgagcgaaa
     7861 aaacgacgtt gacaaaggca tcaccagttg aattaaggca gtgcctggaa atcgcaaatc
     7921 aacttgccag aagtggaata cgctttgttc cgatcccgat cacagcagat acagaacttc
     7981 atctgtttgg tgaaattctt tcccgaaagc tggatgaact ggaaaagctg gtagaagaag
     8041 ctgacacctc accaatcgca taacaccccc accgacatta aaatatcagg agaaaaaaat
     8101 gaacgccgta ctcacagaat tgaacaaatt aggaaaagca acagccgaca gcatttctaa
     8161 aggtctcaat attgatttga atgacgtcat tgatacgctg tggaagttga aaaaccaggg
     8221 gatcgtaaag gtaaaaaacg gcatctggca ggcagtagag ggagttggaa gcaaaacaga
     8281 tattgattca gttcaaccag ttcaaccagt tcagccagtt cagccagtgc agaacaacat
     8341 tataggtgac ctgctacgta aatcacggaa agaagcgcgc cgcgccgggc ggaaacagaa
     8401 acgatgggag ggtgcatgta aggcgttgca agaactgaat aaataccgtg acttgatcaa
     8461 cgaattgtca gagtgaacgt agcgggaatc cacgtccttt agggcgtgga tgatatcagg
     8521 ctgttgaaat acatgtagaa aaggagcgca tactatggaa gtcaacttaa tgaccgaaaa
     8581 agaggtttct gatttgttac aaaaaaggcg cacagctttg tataatttgc gaaaaaaaca
     8641 cggattcccg gaaccagtac ttactcatcc ggcgcgatat agtcgtcagg ccgttgaaca
     8701 atggcttaaa gccggaggaa ttaaccgagc tgtttagcgt gccagaaaat tttatcagca
     8761 tacaactcat acgcatcttt ctgttccgac aaccagtcgt gtttattata cacggccata
     8821 actcccccaa gttcatgccc cagcattttt tcagtgacgt ggggcataat tccttcccca
     8881 gataaattcg ttaccagaga acgcctaaaa tcatgcgttc tccattcagg aatatcaatt
     8941 ttctccctta actttttcat gtacaaatta gccgacgaac gatcaatagg tttatctatt
     9001 tcctgcccgg ggaatagcac atcaaaaccc gcattaagca atctctcgat ataaggttta
     9061 acctgagtga ataccggacg ccggataata ttccccattt ttgaatgctc ttttggggtt
     9121 gtccagacca gatcttccat gttgaactca ctggctgtcg cgagacgtag ctcagaaagt
     9181 ctggcccccc atagcaatag caattgatgt aacaccttgt tggaagtaac tacttttgag
     9241 ttctccagcg ccagccaaat ttttgccagt tccgtatagg taagtacacg actccctaca
     9301 tctggttttt tcccaatatt cttaacacta agcttgagta attcgcatga tgggattaac
     9361 tggcggctga tacaccagtt aataactgaa cgcagttgta acaacaaaac acgagccttt
     9421 ttcttgttga ggctttcttg tttgtcaaag aaacgagccc atgccgatac tgggatactg
     9481 gcaactggcg agccttcaaa ctgtgtgtac atagtgttgt acacgacgga tctgtaaagc
     9541 gtctgtgtat tgggtttcag atcagtaaca tacttatccc accactgatc caggcattct
     9601 tttaatgtca gttcgccgtc actgggcgca aaataatttt tagggttaac tcccttcatg
     9661 tacaatgcgc gcatctcacc gacgataaca cgcgcgtcct tcagtgaagt agacggatag
     9721 cgcccgacag taagacgtac tggcttacca ttccagcgat aacggtactg aaacgtgata
     9781 gttcctgctg gagtaatccg cacacttaac ccgtcaccat cggtgatttc agcagggcca
     9841 gagtagggtt ttccgtagag gcctcgtagt ttggtgtcgc tcagtgccaa tgctttatat
     9901 cctgtacaca agtttataat gtattctgta ctcaatatgt acgcattagc aagtgaacaa
     9961 aacacttttc aacgcaaaag aagataatca accatgaaca aaaacaaact tacaccttga
    10021 ttttaaaacc ataaagacgg tattatcagt catatggtga acaaccaaaa tcaatacgca
    10081 acaatgtcct cttagttaaa tggatataac gagcccctcc taagggctaa ttgcaggttc
    10141 gattcctgca ggggacacca tttatcagtt cgcctccatc cgtaccagtc cgcaaaatcc
    10201 cctgaatatc aagccttccg tagattcaca gttcgtcatg gttcgcgtca gatcgttgac
    10261 agccgcacgc catgacgggt aaaaagtgga taaaataatt ttacccaccg gatttttacc
    10321 catgctcacc gttaagcaga ttgaagcagc aaagccgaaa gaaaaaccat accgcctact
    10381 cgatggtaat ggcctgtacc tttatgtccc tgtatcaggg aaaaaggtat ggcagcttcg
    10441 ctacaagatt gacggtaagg agaaaatcct gaccgtcgga aaatatccgc ttatgacttt
    10501 gcaagaggca agggataaag catggactgc gaggaaagac atctcggttg gcatcgatcc
    10561 ggtaaaggcg aaaaaggctt cgtctaacaa caattccttt agtgcgattt acaaggaatg
    10621 gtacgagcat aagaggcaag tctggtcagc cgcctatgcg actgaacttg caaaaatgtt
    10681 tgatgacgac attttaccta tcatcggcgg ccttgaaatt caggatattg agccgatgca
    10741 actgctggaa gtaatccgca gatttgaaga tcgcggggca atggagcgag ccaacaaagc
    10801 acgcagaaga tgcggcgagg ttttccgtta cgctattgtc accggaaggg ctaaatataa
    10861 cccggcacct gaccttgctg aagccatgaa gggataccgc aagaagaact tcccgttttt
    10921 acctgccgac cagatcccgg cattcaacaa agcacttgca acattttcag gaagtatcgt
    10981 atctctcatt gcgaccaaag ttttacgcta cacagcacta agaacgaaag agcttcgttc
    11041 catgcaatgg aagaacgtcg attttgaaaa caggattatc accatcgagg ccagtgtgat
    11101 gaagggacgc aagattcatg tggttccgat gtcggaccag gttgttgaac ttctcactac
    11161 gctaagctcc atcactaaac cagtatcaga gtttgttttt gccgggcgca acgataagaa
    11221 gaagtcaatc tgtgagaacg ctgtactgct tgtgatcaaa caaatcggct atgaaggtct
    11281 ggaaagcggt cacggattca ggcatgaatt cagcacgatt atgaacgagc acgaatggcc
    11341 tgctgacgct attgaagtgc aactagcaca tgccaacggc ggatctgtgc gtgggattta
    11401 caaccatgct cagtatctcg ataagcgcag agaaatgatg cagtggtggg cggactggct
    11461 tgatgggaag gtggagtagt tattacaacg attcttataa aaaagaaaag ccttgcacat
    11521 atagcaaagc ttttcacttc tcaggatttc ttacaccaag aaaccatcaa caataacaaa
    11581 atgcaattat tatatgttaa ctacatatgc atttcattaa aaccaatcat aaatgcttat
    11641 catactttaa ttgctataat tgattgcctt ctattttttc aactcgagtg gttgaaacat
    11701 cattcttaaa tccactccac cattttctta gcttattgtt tattggcttt tca
//
LOCUS       DBFIFM010000139        11722 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.139, whole genome shotgun sequence.
ACCESSION   DBFIFM010000139 DBFIFM010000000
VERSION     DBFIFM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.139"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..435
                     /gene="decR"
                     /locus_tag="N7C74_003337"
     CDS             <1..435
                     /gene="decR"
                     /locus_tag="N7C74_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HGU2433148.1"
                     /translation="LLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLEDDGILIGKV
                     ALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGEYDYLMRVQ
                     VADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            465..2237
                     /locus_tag="N7C74_003338"
     CDS             465..2237
                     /locus_tag="N7C74_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HGU2433149.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENREEAVDA"
     gene            2230..4011
                     /locus_tag="N7C74_003339"
     CDS             2230..4011
                     /locus_tag="N7C74_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HGU2433150.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            4192..4530
                     /gene="glnK"
                     /locus_tag="N7C74_003340"
     CDS             4192..4530
                     /gene="glnK"
                     /locus_tag="N7C74_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HGU2433151.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            4560..5846
                     /gene="amtB"
                     /locus_tag="N7C74_003341"
     CDS             4560..5846
                     /gene="amtB"
                     /locus_tag="N7C74_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HGU2433152.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(5895..6755)
                     /gene="tesB"
                     /locus_tag="N7C74_003342"
     CDS             complement(5895..6755)
                     /gene="tesB"
                     /locus_tag="N7C74_003342"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HGU2433153.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            6973..7545
                     /locus_tag="N7C74_003343"
     CDS             6973..7545
                     /locus_tag="N7C74_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HGU2433154.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(7578..7889)
                     /locus_tag="N7C74_003344"
     CDS             complement(7578..7889)
                     /locus_tag="N7C74_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HGU2433155.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            8052..8148
                     /gene="ffs"
                     /locus_tag="N7C74_003345"
     ncRNA           8052..8148
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="N7C74_003345"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            8268..8621
                     /locus_tag="N7C74_003346"
     CDS             8268..8621
                     /locus_tag="N7C74_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HGU2433156.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(8663..10213)
                     /gene="pdeB"
                     /locus_tag="N7C74_003347"
     CDS             complement(8663..10213)
                     /gene="pdeB"
                     /locus_tag="N7C74_003347"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HGU2433157.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGADCSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASSKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(10377..10847)
                     /locus_tag="N7C74_003348"
     CDS             complement(10377..10847)
                     /locus_tag="N7C74_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HGU2433158.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(10963..11514)
                     /gene="maa"
                     /locus_tag="N7C74_003349"
     CDS             complement(10963..11514)
                     /gene="maa"
                     /locus_tag="N7C74_003349"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HGU2433159.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     ALGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
BASE COUNT         2736 a         2856 c         3230 g         2900 t
ORIGIN      
        1 ctgctggcct tactacagca ggattgcacc ctctctttgc aggcactggc tgaagccgtt
       61 aatctgacaa ccaccccctg ctggaagcgc ctgaaacggc tggaggacga cggtatcctt
      121 atcggcaaag tcgccctgct ggatccggaa aaaataggcc tcggcctgac cgcctttgtg
      181 ctgataaaaa cgcaacatca cagcagcgaa tggtattgcc gctttgtcac ggtggttacc
      241 gaaatgccag aagtgctggg gttctggcgc atggctggtg aatacgatta tctgatgcgc
      301 gtccaggttg ccgacatgaa acgctacgac gagttttata agcgtctggt aaacagcgtg
      361 ccggggctgt cggacgtcac ttccagcttc gcgatggaac agattaaata caccacttct
      421 ttacccatcg aataaatatc cagaatcagg tcaggacaca acgcgtgcga ttatttgctc
      481 aattaagctg gtatttccgt cgggaatggc gtcgctatct cggggctgtc gccttgcttg
      541 tcattatcgc gatgctgcaa ctggttccac caaaagtggt tggtattgtt gtcgatggcg
      601 tgacagaaca acactttact accgggcaga tcctgatgtg gatcgccacc atggtgctga
      661 ttgccgttgt ggtttatctc ctgcgttacg tctggcgcgt attgctgttt ggtgcgtctt
      721 atcaactggc tgttgaactg cgtgaagatt attaccgtca gctaagccgg cagcatcctg
      781 agttttacct gcgtcatcgc acgggtgacc tcatggctcg tgcgactaat gacgtcgatc
      841 gcgtcgtgtt tgccgccgga gaaggggtgc tgacgctggt ggattcactg gtaatgggct
      901 gcgctgtgtt gattatgatg tctacgcaaa ttagctggca gttgacctta tttgccctgt
      961 taccgatgcc agtgatggcg atcatgatta agcgcaacgg cgatgctttg catgaacgct
     1021 ttaagctggc acaggcggcg ttttccagtc ttaatgaccg cacccaggaa agcctcacca
     1081 gtatccgcat gatcaaagcc tttggtctgg aagatcgcca gtcggcgtta tttgccgcgg
     1141 atgccgaaga taccggcaaa aaaaacatgc gggtggcgcg tattgatgct cgtttcgacc
     1201 cgaccatcta tatcgcgatt ggtatggcga acttgctggc gattggcggc ggtagttgga
     1261 tggtggtgca gggcagttta acgctgggcc agctcaccag ttttatgatg tatttaggtc
     1321 tgatgatttg gccaatgctg gcgctggcat ggatgtttaa cattgtggaa cgtggtagtg
     1381 ctgcgtacag ccgtattcgc gcgatgctgg cggaagcgcc ggtggtgaac gatggtagcg
     1441 aacccgtgcc ggaagggcgt ggcgaactgg atgtaaatat tcaccagttc acgtatccgc
     1501 agactgacca tcctgcgctg gaaaacgtca atttcgccct gaaacccggt cagatgctgg
     1561 gtatctgcgg gccgactggt tccggcaaaa gtaccctgtt gtcgctcatt cagcgtcatt
     1621 tcgacgtcag cgagggggat attcgctttc atgatattcc tctgacgaag ttacaactcg
     1681 atagctggcg tagccgcctg gcggtagtta gccagacgcc attccttttt tctgacaccg
     1741 tggcgaataa catcgcgctg ggttgcccga atgccaccca gcaagagatt gagcatgtcg
     1801 cgcggttagc cagcgtacat gacgatattt tgcgtctacc gcaaggttac gatacagagg
     1861 tgggcgagcg cggtgtgatg ctttccggcg ggcaaaaaca gcgtatctcc attgctcgtg
     1921 cgttattagt caacgcggaa atcctcatcc ttgatgatgc gctttcggcg gtggacggac
     1981 gcactgagca ccagatcctg cataacctgc gtcagtgggg gcagggaaga acggtaatca
     2041 tcagtgccca tcgcctttct gcactgacgg aagccagtga aattattgtg atgcagcacg
     2101 gacatatcgc ccagcgtggc aatcatgatg tgctggcaca acaaagcggc tggtatcgcg
     2161 atatgtatcg ctatcaacaa ctggaggcgg cgctcgacga cgctccggaa aatcgcgagg
     2221 aggccgtcga tgcgtagttt tagccaactg tggccgactc tcaagcgcct gttagcgtac
     2281 ggttcaccgt ggcgtaaacc gctggggatt gcggtcctga tgatgtgggt tgcggcggcg
     2341 gcagaagtca gtgggccgct gcttatcagc tattttatcg acaatatggt agcgaaaaat
     2401 aacctgccgt tgaaagtggt tgcagggctg gctgcggcgt atgttgggct gcaactgttt
     2461 gccgccgggc tacattacgc gcagtcgctg ctgtttaatc gggcggcagt aggcgtagtg
     2521 caacagttgc gtaccgacgt gatggatgct gcgttacgcc agccattaag cgagtttgat
     2581 acccaacccg tcgggcaggt gatttcccgc gtcactaatg acactgaagt gatccgcgat
     2641 ctctacgtta ccgtagtggc aactgtcctg cgcagtgccg cactggtggg cgcgatgctg
     2701 gtggcgatgt tcagcctcga ctggcgaatg gcgctggtgg cgataatgat tttcccggtg
     2761 gtgctggtgg taatggtgat ataccagcgt tacagcacgc caattgtccg tcgtgtgcgc
     2821 gcctatctgg cggatatcaa cgacggcttt aacgaaatca tcaatggcat gagcgttatc
     2881 cagcagtttc gtcagcaggc gcgatttggc gaacgtatgg gtgaggccag tcgttcacac
     2941 tatatggcga ggatgcaaac cctgcgcctc gacggttttc tgctgcgtcc gctgctgagt
     3001 ctgttttcat cgctcattct ctgtggcttg ttgatgctgt ttggcttctc cgccagcggc
     3061 accattgaag tgggcgtgtt gtatgcgttt atcagctatc ttgggcgact taacgaaccc
     3121 ttaatcgaac tgaccacgca gcaggcgatg ctgcaacagg ctgttgttgc tggtgagcgc
     3181 gtgtttgagc tgatggacgg accgcgccag caatatggca atgatgatcg cccgttacag
     3241 agtggcacca tcgaagtcga taacgtgtca tttgcttatc gcgatgacaa tctggtgcta
     3301 aagaacatta atctctctgt gccttcgcgt aactttgtgg cgctggtcgg gcataccggc
     3361 agtggtaaaa gtaccctcgc cagtttattg atgggctatt acccgctaac ggaaggtgag
     3421 attcgccttg atggtcgtcc attaagttcg ctaagtcaca gcgcgctgcg ccagggcgtg
     3481 gcaatggtgc agcaagatcc ggtggtgctg gcggatacct tcctcgccaa cgtgacgctg
     3541 gggcgggata tctccgaaga acgcgtctgg caggcgctgg aaaccgtgca actggcggag
     3601 ctggcacgca gtatgagcga cgggatttat acgccgctgg gcgagcaggg gaataatctc
     3661 tctgtcgggc aaaagcaact gctggcactg gcgcgcgtgc tggtcgagac gccgcaaatc
     3721 ctgatccttg atgaggcaac cgccagcatt gactccggta ctgaacaggc gattcaacat
     3781 gctctggcgg cggtgcgtga acataccacg ctggtagtga ttgctcaccg cttatcgact
     3841 attgttgatg ccgacactat tctggtgctt catcgtgggc aagccgtgga gcagggcact
     3901 caccagcaac tgctggcggc ccagggacgc tactggcaga tgtatcaact gcaacttgcg
     3961 ggcgaagagc ttgcagccag cgtgcgtgaa gaagaatcat tgagcgcctg aatagcgcaa
     4021 tatctcatcg ttggtgcaaa aatgtaacgc actgtgcact gtcatagtgc gttttcattt
     4081 tcaatcttct taacttcctg ctctctttct cgtttttcat ttctggcaca ccgcttgcaa
     4141 taccttcttc gtgtagcaga accattaccg aattctgacc ggaggggatc tatgaagctg
     4201 gtgaccgtga taatcaaacc attcaagctg gaagacgttc gtgaagcgtt atcttccatt
     4261 ggtattcagg gcctgaccgt caccgaagtg aaaggtttcg ggcgtcagaa agggcatgcc
     4321 gagctgtacc ggggggcgga atacagcgtc aacttcctgc caaaagtaaa aattgatgtg
     4381 gcgattgctg atgaccaact cgatgaagtg atcgatatcg tcagtaaggc ggcttacacc
     4441 ggaaaaattg gcgacggcaa aatcttcgtc gctgaattgc aacgcgtcat tcgtattcgt
     4501 accggcgaag ccgacgaagc ggcgctgtaa tctctggcac acagcaacag gaacgaaaaa
     4561 tgaagatagc gacgataaaa actgggcttg cttcactggc gatgctgccg ggactggtaa
     4621 tggctgcacc tgcggtggcc gataaagccg acaatgcgtt tatgatgatt tgtactgcac
     4681 tggtgctgtt tatgactatt ccggggattg ccctgtttta cggtgggttg attcgcggca
     4741 aaaacgtgct gtcgatgctg acgcaggtga cggtgacgtt tgcactggtc tgtattctct
     4801 gggtggttta cggttactcg ctggcgtttg gtgagggcaa caacttcttc ggcaacatta
     4861 actggttgat gctgaaaaac atcgaactga cggcggtgat gggcagcatt tatcagtata
     4921 tccacgtagc gtttcaggga tcgtttgcct gcattaccgt aggcttgata gttggggcgc
     4981 tggcggaacg aatccgcttc tcagctgtgt tgattttcgt ggtggtatgg ctgacgctct
     5041 cttacattcc tattgcgcat atggtgtggg gcggtggttt gctggcttct cacggtgcgc
     5101 tggatttcgc gggtggcacg gtggtgcata ttaacgccgc aatcgccggt ctggtgggcg
     5161 cgtatctgat aggcaaacgc gtgggcttcg gtaaagaggc gtttaaaccg cacaacctgc
     5221 cgatggtctt caccgggact gccattctct atatcggttg gtttggcttt aacgccgggt
     5281 cagcgggcac ggcgaatgaa atcgcggcac tggcatttgt gaatactgtg gtcgcaacgg
     5341 cggcggcaat tcttggctgg atcttcggtg aatgggcgct gcgtggtaag ccttcactgc
     5401 tgggagcgtg ttctggcgcg attgccggtc tggtcggcgt gacgccagcg tgtggctaca
     5461 ttggtgttgg cggcgcgttg attatcggcg tggtagctgg tctggcgggc ttgtggggcg
     5521 ttaccatgct caaacgcttg ctgcgggtgg atgatccctg cgatgtcttc ggtgtgcacg
     5581 gcgtttgtgg cattgtcggc tgtatcatga ctgggatttt tgccgccagc tcgctgggcg
     5641 gcgtgggctt cgctgaaggt gtgacgatgg gccatcagtt gctggtacag ttggaaagca
     5701 ttgccattac gattgtgtgg tctggtgtgg tggcatttat cggctacaaa ctggcggatc
     5761 tgacggttgg tctgcgtgtg ccggaagaac aagagcgaga agggctggac gtcaacagtc
     5821 acggcgagaa tgcctataac gcataacaag cactgcaaaa aacagccgga cggttttcac
     5881 ctccggctat ttttttaatt gtgattacgc atcacccctt cctgaacggt cgaggcaacc
     5941 agtacgccgt cctgggtata aaactcaccg cgcacaaagc cacgtgcgct ggacgccgag
     6001 gtgctctcca cgctatacag cagccattca ttcaaattaa acgggcgatg gaaccacatg
     6061 gaatggtcaa tggtggcaat ctgaatcccc ggttcgagaa aaccgatacc gtgcggctgt
     6121 agagctaccg gcaggaagtt aagatcagaa gcgtaaccga gcagatactg atgaacgcgc
     6181 aggtcatccg gcacgctacc atttgcgcgg atccacacct gacgatgtgg ttctgcgacg
     6241 tgacctttca gtgggttatg aaactccacc ggacggactt ccagcggacg atcgcagatg
     6301 aatttatctt taagcactgg cggcagcagg tgcgccagcg attgggcgat ttgcgtttcc
     6361 gaaggcaggc catcaggcgc tggcgcggac ggcattgttt tttgatgttc gaaacccgct
     6421 tctggtgcct ggaaagaggc cgtcatataa aaaatcggtt tgccgttttg aatagcagca
     6481 acccggcggg cgctgaagct gttaccgtca cgcagcgttt cgacatcata aataatcggc
     6541 ttcttactat cgccagggcg aagaaagtag ctgtgaaacg aatgtaccag ccgctcttca
     6601 gggacggtct cttttgcagc atacaaggcc tgacccacga cctggccgcc aaatacctgg
     6661 cgtaaaccta aatcttcact ctggccgcga aagagtcctt cctcaatttt ttccagattt
     6721 aacaatgtca gtaaattttt tagcgcctga ctcatataac tctccagtaa caaagctgcc
     6781 gcagcaagcc aaagtgagtt gagtataacg caaatttgct actggtccga tgggtgcaat
     6841 ggtctgaatt acgggctaat tacaggcaga aatgcgtgat gtgtgccaca attgttgacg
     6901 ttactatttt gttaaccact cttccggcga ggaaagttag cccgctggtg cattgataat
     6961 aaggagaaat gaatgaaact cgtgcacatg gccagtggtt tagcggttgc gattgcgttg
     7021 gcggcttgcg cagataaaag cgcggatatt cagacgccag ccccggctgc aaatacgtct
     7081 atttcagcaa cacaacaacc tgctatccag caaccgaatg tctccggtac cgtctggatc
     7141 cgtcagaaag tcgcactgcc gcctgatgct gtgctgaccg tgacactttc tgacgcgtcg
     7201 ttagccgatg caccgtcaaa agtgctggcg cagaaagcgg tgcgtactga aggtaaacag
     7261 tcaccattca gctttgttct gccatttaac ccggcagatg ttcagccgaa cgcgcgtatt
     7321 ctgttgagtg cggcgattac cgtgaatgac aaactggtat ttattaccga taccgttcag
     7381 ccggtgatca accagggggg aaccaaagcc gacctgacat tggttccggt tcagcaaacc
     7441 gccgtgcctg tccaggccag tggtggcgca acaactaccg taccatcgac ttcaccgacc
     7501 caggtgaatc catcttcggc agttcctgcc cctacgcaat actaagcgtt actaacccct
     7561 ttccatctgg agaggggtta gtagttccag cgatagcgct gtaagtcgat ttgcccgctt
     7621 cccgatacca tcacaccttc tgccagtaat gcctgtcgtt gacgctgtaa atccggtccg
     7681 gttagcgaaa tggtgccgtg gcgattaacc acccggtgcc agggcaaggt gctgccttcg
     7741 gggagacgct ttaacacacc gccaacctgg cgcgcggcgc ggggcgatcc cgccagtttc
     7801 gccacatcac cgtaagtggt gacataccct tcgggaatag cggctacgat ttgccagacg
     7861 cgttggggaa atgaatcttc tttttccatc ttttcttcct gagataattt ttcagcataa
     7921 tctggaaaaa cgcccgagtg aagccgcatt gcgcaagaaa ccagcatctg gcacgcaatg
     7981 ggttgcaatt agccggggca gcagtgataa tgcgcctgcg cgttggttct caacgctctc
     8041 aatgggggct ctgttggttc tcccgcaacg ctactctgtt taccaggtca ggtccggaag
     8101 gaagcagcca aggcagatga cgcgtgtgcc gggatgtagc tggcagggcc cccacccatt
     8161 tctgcctccc accgtttcgt caaaaaatcc caacatgact aaactttaac cacaactgac
     8221 gtcgcaagaa ttgtctggct gcgcagtacg cttcggaggt atgtctgatg aagtatgttg
     8281 atggttttgt ggttgccgtt cctgccgata aaaaggatgc ctatcgggaa atggccgcta
     8341 aggccgcgcc attgtttaaa gagtttggcg cgcttcgtat tgtcgaatgc tgggccagcg
     8401 atgtaccgga tggcaaagtg accgattttc gtatggcggt gaaagcggaa gagaatgaag
     8461 aggttgtctt tagctggatt gaataccctt caaaagaggt ccgcgacgca gctaatcaaa
     8521 agatgatgtc ggacccacgg atgaaagagt tcggcgagtc catgccgttt gacggcaagc
     8581 gaatgatcta tggcggattc gagtcaatca tcgacgaata ggtagcgtga cgggctgtgc
     8641 ggcgtgccgt cagcccaaaa attcacaaat gttgctcggc ccagcgtaag aaatcttctt
     8701 tcggtaatgc cttgctgtag agccagccct gaccgtagtg cacgccatgc tggcgtagcc
     8761 actcttcttg tttactggtt tcgattccct ccgctaccat tttcagtttc agtgtttttg
     8821 ccatttcgat gatatgcggc gtgacatttt tatattccag cgcatcaacg aaagatttat
     8881 cgatcttcag aatgtcgaca tccagatcct gtaaataact taaacttgaa taccccgtac
     8941 caaaatcatc aagataaatt tcatggcccg cctcccggta gcgagaaatt atcggggcgc
     9001 tggttttcgg atcggcaaac tcgcgttcag tgagttcaag cgcgatctgt ctgggattaa
     9061 cctgatagtg attgatcatt tcacgcagca attgcgggat tttttccgag gtgagcacgg
     9121 tggattcaag attgatcgaa atatgctgct gtggatgctg acgcagccag tcgcccatat
     9181 cttcaaagac gcttcttata atcagtagcg tcaatggctc agaaaggccc gtttgctgtg
     9241 ccagcggaat aaaactatct ggtgacaacc aactaccgtc tgtctgcggc cagcgcgcca
     9301 gtgcctcagc accgacaatt ttgccattgg ctaaggagac aatcggctga tagtgcacgc
     9361 aaatatcacg attttcgata gcatcctgca gccgatgatg cggtgactgg atgcggcgca
     9421 aaatgcgcag cacaaacatc gctgccagca ggccaatcac caacccgagc ggtaaccaaa
     9481 taaagacttg ccgatgccag cctttctgca acattttcga tgaagcccag gtgataattg
     9541 aaatattcat ctccgggaag ggcaggatat cgtaaatgat tccattattt tcgatatgct
     9601 caccgggtgt tttttgtagc ctggtaataa ttccctgagc aatttcatcg ctgctggtta
     9661 tgacaacgtt atgggcattg ccaataatgg cggcatcaat ttgccatgag ctatagggaa
     9721 tgacatcaat aaaggaagcg gggtcgatca tgacgacata atgtgccgtt cccatggcga
     9781 ccatgtaacg gataatgcct aaatcgttat gtgatgttaa ccagacacga taaccatctt
     9841 tcgaaatttt acctggctcg gggaaggtat cgggcggact ttcatgctcc agagacgaac
     9901 actggggaac gttgttatcg atataaacca cttcctgaat atagcgataa ctgtaagata
     9961 cccgacgcat ttccatgaga tgggcttcac tacagtcagc gccttgccat ctttccagct
    10021 cttgcagcgc atctttccct tgtgtcgcca ccttattggc ccgaatagcg acgcgggagg
    10081 aataggtatc cagctcttca ataaacgatg tttctacctg ctgatgggcc agccagatgc
    10141 ttaagccgac aggcagcaat actgaaagaa tcagtactcc cgaaataagg ccgaccagat
    10201 gtcgtgttct caccagcata tccttattaa cacagtgtag ggaaagtata tctgattgtg
    10261 gaaaaaggct ggcaggagat aaggaggtag agctggtatt tcatgaagtg atgggtggaa
    10321 acgatcgttg tcatttgacc aggcgcagag ctttacgcct ggtatgtaat cctaacttaa
    10381 gatgttgatt cggcgcgagc gagggtaaaa atatcgtaaa aagacaattc acctttacgg
    10441 acgatcattt tttgcagttg ttccttatga acatccgcga cacgcggaga gttcaagatg
    10501 gcttcaacca gcgcggcagg tacaaagcgc gtgttgtacc attcgccgtt atagcaaacg
    10561 cggaaatcca acacgtcgat atcttcatag cggtagcgtg ggtagacatc gaaaaagaag
    10621 aaaccattaa gcaggataaa taatacaacc agtagccaga cagagcctga aagcgtttcg
    10681 gactgcaaca tcaccgccag ggtggcaaaa aaagcaacgt acataccgat aaacagcccc
    10741 ggatgtttac ggataaaact gatactaaag cggggtttgt tgtcgcgttt ttcgcgggta
    10801 ttcagagact caatcgtttc ggtcagcagg cgttgtattt cggtcatcgt ttgccttcgt
    10861 ggtatctgca actttccagg gaacacccta ttttaacgtg ggaatagtag agggaaagta
    10921 acagatctgc cacaattttg cataacagtt gcgaaaaacc gattacaatt ttttaattat
    10981 tctggctgga ttaccgccca cgacaacgtt gtccgggaca tcttttgtga caactgcacc
    11041 tgaggctacc acgacgttat caccaatggt cacaccaggg ttaatgaccg cgcgtccgcc
    11101 aatccagacg ttattaccga tggtgacggg tttccccagt tcagcaccgc tattacgtgc
    11161 tacagggtcg atgggatgtg ttgccgtgta gatatgaacg cctagtgcca acatacagtt
    11221 atcaccgatg cgaatagggc agacatcaag catcacgcaa tcgaagttgg cgaaaaaatt
    11281 attaccgaga aaaatgttat agccatagtc acagcgaaac gttggctcaa tataagcctc
    11341 tgtcacctga ccgaatagat cagcgagaat ttgctggcgt aatgtgtgct cttccgccag
    11401 ggaatgattg tatcggtgaa taagctgacg agcgcgcagg cgatcgcgag ataacgtctc
    11461 atctgccgag cgatacaact caccagcaat catcttttct ttttctgtgc tcattactga
    11521 cctccggttg ctgaaaatgc ggcaacacag taagacgaaa agtcagtgaa gagaacgttc
    11581 ccattacatc tgtgtgataa agatcacata gggtatgtct tcatggcgaa aaagtataaa
    11641 attcttaata aacagccggt tatagctccg aaagcgaatt atttattagc gaataaattt
    11701 ccatactgag gaagggatct tg
//
LOCUS       DBFIFM010000140        11696 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.140, whole genome shotgun sequence.
ACCESSION   DBFIFM010000140 DBFIFM010000000
VERSION     DBFIFM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.140"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            76..678
                     /gene="yebB"
                     /locus_tag="N7C74_003350"
     CDS             76..678
                     /gene="yebB"
                     /locus_tag="N7C74_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HGU2433160.1"
                     /translation="MNINYPAEYEIGDKVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(680..1201)
                     /gene="ruvC"
                     /locus_tag="N7C74_003351"
     CDS             complement(680..1201)
                     /gene="ruvC"
                     /locus_tag="N7C74_003351"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HGU2433161.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1236..1976)
                     /gene="yebC"
                     /locus_tag="N7C74_003352"
     CDS             complement(1236..1976)
                     /gene="yebC"
                     /locus_tag="N7C74_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU2433162.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(2005..2457)
                     /gene="nudB"
                     /locus_tag="N7C74_003353"
     CDS             complement(2005..2457)
                     /gene="nudB"
                     /locus_tag="N7C74_003353"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HGU2433163.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2575..4347)
                     /gene="aspS"
                     /locus_tag="N7C74_003354"
     CDS             complement(2575..4347)
                     /gene="aspS"
                     /locus_tag="N7C74_003354"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HGU2433164.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLRSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPAALAELSIQVVKKAENN"
     gene            4657..5223
                     /gene="yecD"
                     /locus_tag="N7C74_003355"
     CDS             4657..5223
                     /gene="yecD"
                     /locus_tag="N7C74_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGU2433165.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            5220..6038
                     /gene="yecE"
                     /locus_tag="N7C74_003356"
     CDS             5220..6038
                     /gene="yecE"
                     /locus_tag="N7C74_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HGU2433166.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNQVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            6091..6486
                     /gene="yecN"
                     /locus_tag="N7C74_003357"
     CDS             6091..6486
                     /gene="yecN"
                     /locus_tag="N7C74_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HGU2433167.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6527..7270
                     /gene="cmoA"
                     /locus_tag="N7C74_003358"
     CDS             6527..7270
                     /gene="cmoA"
                     /locus_tag="N7C74_003358"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HGU2433168.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7267..8238
                     /gene="cmoB"
                     /locus_tag="N7C74_003359"
     CDS             7267..8238
                     /gene="cmoB"
                     /locus_tag="N7C74_003359"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HGU2433169.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8403..10832)
                     /gene="torZ"
                     /locus_tag="N7C74_003360"
     CDS             complement(8403..10832)
                     /gene="torZ"
                     /locus_tag="N7C74_003360"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HGU2433170.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGYNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFAELAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAQLRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(10857..>11696)
                     /locus_tag="N7C74_003361"
     CDS             complement(10857..>11696)
                     /locus_tag="N7C74_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HGU2433171.1"
                     /translation="SKDIYHEFVSGKIDSDDKFEAHRQEMAETVWKELKATDSATCRS
                     CHSFDAMDIASQSESAQKMHNKAQKGGETCIDCHKGIAHFPPEIKMDDNAAHELESQA
                     ATSVTNGAHIYPFKTSRIGELATVNPGTDLTVVDASGKQPIVLLQGYQMQGSENTLYL
                     AAGQRLALATLSEEGIKALTVNGEWQADEYGNQWRQASLQGALTDPALADRKLLWQYA
                     EKLDDTYCAGCHAPIAADHYTVNAWPSIAKGMGARTSMSENELDILTRYFQYNAKDIT
                     EKQ"
BASE COUNT         2642 a         3105 c         2880 g         3069 t
ORIGIN      
        1 tacattgcgc ctcctgaaaa aataaatttt ttatgctatt acgtatattc gtatctattt
       61 caatggaatg acaacgtgaa tattaattac cctgctgaat atgaaattgg agataaagtc
      121 tttacatgta taggtgctgc cttatttggt caaatatcag ctgcatcaaa ttgctggagt
      181 aatcacgtcg ggatcattat cggtcataac ggtgaagact tcctggttgc agaaagccgg
      241 gttccccttt caaccatcac cacgctatct cgttttatta aacgttcgtc taatcaacgc
      301 tatgctataa agcgattaga cgctgggctg acagaacaac aaaaacaacg aattgttgaa
      361 caggttccct cccggctacg caaactttac cacaccggtt ttaaatacga atcttcgcgc
      421 cagttctgtt caaaatttgt ttttgatatt tataaagaag cgctatgtat tccggtgggt
      481 gaaatagaga cgtttggaga attgttaaat agcaatccga atgcaaaact cactttctgg
      541 aaattctggt tcctgggttc tattccgtgg gagcgtaaaa ccgtcacgcc agccagtttg
      601 tggcatcatc caggtttggt gttgattcat gcggagggag tggaaacgcc tcagccggaa
      661 ctgaccgagg cggtataact taacgcagtc gccctctcgc caggttcagc cgcgattcgc
      721 tcatctgcat cgcattctga ctaacgtggc agtgggtgat ggcaatcgcc agcgcatcgg
      781 cggcatccgc ctgtggatta gcgggcagtt tcagcaaggt gcggaccata tgctgcacct
      841 ggcttttttc ggcactacca atacctacca ccgtttgctt tacctgacgt gccgcgtatt
      901 caaatactgg caactcctga ttcaccgccg ccacaatcgc cacgccgcgc gcctggccca
      961 gtttcagggc tgagtcagcg ttctttgcca taaagacttg ttcaatggcg aaataatcgg
     1021 gctggaactg ggtgatgatt tccgtcacgc ccgcatagat gagtttcaga cgagacggta
     1081 aatcatccac tttggtgcgt atgcatccgc tacccaggta ggacagttgc ctacctacct
     1141 ggcggatgac gccgtagccg gtcacgcgcg aacccggatc aatgccgaga ataatagcca
     1201 tcacgcgtct ccgttttgct gtttagcagg cctcatcaga gagtcgctgc gacctcatca
     1261 gagatttcac cgttatggta aacttcctgc acgtcgtcgc aatcttccag catatcgatc
     1321 agacgcatca gtttcggtgc ggtttctgca tccatatcag ctttggtaga cgggatcatg
     1381 gaaacttccg cgctgtctgc tttcagacct gccgcttcca gagcgtcgcg cactttaccc
     1441 atttcttccc atgcagtgta gacatcaatc gcgccgtcat cataggtcac aacgtcttca
     1501 gcacctgctt ccagtgctgc ttccatgatg gtgtcttcat cgcctttctc gaaggagatg
     1561 acgccttttt tgctgaacag ataggctacg gaaccatcag taccgaggtt accgccacat
     1621 ttgctgaatg catgacgcac ttcagcaacg gtacggttgc ggttgtcaga cagacattca
     1681 atcatgattg ccgtgccgcc aggaccgtaa ccttcgtaga tgatggtttc catgttcgca
     1741 tcatcatcac cgcccacacc acgtgcaatt gcgcggttca gagtgtcacg ggtcatgttg
     1801 ttagacagtg ctttatcaat tgctgcacgc agacgcgggt tagcgtccgg atcgccaccg
     1861 cccagcttag ccgcggttac cagctcacga atgattttag tgaagatttt accgcgctta
     1921 gcatcctgcg cagctttacg atgtctggtg ttggcccatt tactatgacc tgccataaaa
     1981 atatctccag atagccctgc ctgttcaggc agcgttaatt acaaactgtt caatcgcctg
     2041 ccggttgctc caggacttag tgagcgccgc cgcagcaggc gcatcaagcc acttgtaagc
     2101 cagatgttca gtgaaaacga tctgccgctc atgcggaagc gcaagacaga accatgattc
     2161 cgtattacgc gtcacgcccg gcgcatagcg atgacgtaaa tgtgaaaaaa tttcaaactc
     2221 taccgtgcgc tgacagtcaa ttaaggtcag ttgttcagcg acaacatcaa tggtgacctc
     2281 ttcctttact tcgcgcatgg cagcttgcgg cgcggtttca ccctcttcca cgctgccggt
     2341 taccgactgc cagaaatcgg ggtcatcacg ccgctgcaac atcagcaccc gtttcgtatc
     2401 ttgtgcgtag atgaccacta agatcgaaac gggacgctta tacactttat ccttcacgct
     2461 gcctcttcgt tgactgcctt cgctcatccc attcacatag ttatctatgc tcatgggagt
     2521 tcactcagtt gccgcctcga tgcaacgcga atgatttcgt gtatttgagt catatcagtt
     2581 attctcagcc ttcttcacaa cctgaatgct cagctcagcc agtgcagccg ggttagcaaa
     2641 gctcggtgct tcagtcatca gacacgctgc cgccgtggtt ttcgggaagg cgataacgtc
     2701 acggatattg tcggtgccgg tcagcagcat ggtcagacgg tcaagaccga atgccagacc
     2761 tgcgtgcggc ggagtaccgt atttcagagc gtcgagcagg aagccgaatt tctcgcgctg
     2821 ttcctcttcg ttgataccca gaataccaaa caccgtctgc tgcatatcac cattatggat
     2881 acgtactgaa ccaccgccca cttcgtaacc attgatgacc atatcgtaag cgttcgccac
     2941 cgcattttcc ggtgcagctt tcagctctgc agccgtcatg tctttcggtg aggtgaacgg
     3001 atggtgcatt gccgtcaggc cgccttcacc gtcgtcttca aacatcggga agtcgataac
     3061 ccacagcggt gcccatttgc tttcgtcggt cagaccaagg tctttaccca ctttcaggcg
     3121 cagtgcgccc atcgcgtcgg caacaatttt cttgttgtcg gcaccgaaga aaatcatatc
     3181 gccatcttgc gcggcagtac gatccaggat ggcttcgatg atttctgcat taaggaactt
     3241 cgctaccggg ctgttgatac cttccagacc tttcgcgcgt tcgttaactt tgatgtaagc
     3301 cagacctttc gcgccgtaga ttttaacgaa gttaccgtat tcgtcgatct gcttacgggt
     3361 cagcgatgcg ccacccggaa cacgcagagc ggcaacgcga cctttcggat cgttcgccgg
     3421 acctgcaaat actgcaaact caacagatct cagcagatca gcaacgtcgg tcagttccat
     3481 cgggttacgc agatccggtt tatcagaacc ataacggcgt tcggcttccg caaaggtcat
     3541 tactgggaaa tcgcccagat ccacgccctt cacttccagc cacagatggc gcaccagcgc
     3601 ttccatcact tcacgcactt gcggcgcggt catgaaagaa gtttccacat cgatctgagt
     3661 aaattcaggc tgacgatcag cgcgcaggtc ttcgtcacgg aagcatttaa cgatctgata
     3721 gtagcggtca aagccggaca tcatcagcag ctgtttgaac aactgtgggg attgcggcag
     3781 tgcgtagaat ttacctttgt gcacacgaga aggcaccagg tagtcacgcg cgccttccgg
     3841 cgtggctttg gtcagcatcg gagtttcgat gtcgaggaaa ccgtggtcat ccataaaacg
     3901 gcgcaccagg ctggtgattt tagcgcgggt tttcaggcgc tgagccattt ccggacgacg
     3961 caggtcgagg tagcggtatt tcagacgcgc ttcttcggtg ttgacgtggt tagagtcaag
     4021 cggcagaaca tctgcgcggt tgatgatagt cagcgaggac gccagcactt cgatttcgcc
     4081 ggtcgccata tcgcggttaa tatttttttc gtcacgcgca cgtacggtgc ccgtgacctg
     4141 aatgcagaac tcattacgca gttcagaggc cagctttaac gcgtccgcac gatccggatc
     4201 gaaaaatacc tgcacgatac cttcgcggtc gcgcatatcg atgaagatca ggctaccaag
     4261 atcacgacga cggttgaccc aaccacacag agtcacctgc tgccccacgt gggacaaacg
     4321 gagctgtcca caatattctg tacgcatgag atatccctta acttagctgc cggcggatgc
     4381 cccctgctgc gcaggtgacc aagtcgcagc gttagctgta tgtcacaact gaatgaaaaa
     4441 aggcggctat tatactggaa attctgccgc accgtaagag cctggcccgc gctggaacgc
     4501 ctcgttacca ctttatatcg ggcctgaaat cagactctac gccagtttgc tataaaggtg
     4561 ttgcccgaac tcatacaaat taacaaaatt tgtcgttccg ccatcggcta atcgcattaa
     4621 ggtgtgaggc acgattttgt tttttcagga gtcatcatgc ttgaacttaa tgctaaaacc
     4681 actgcgctgg tggtgatcga tttacaagaa ggcatcttac cttttgccgg tggtccacat
     4741 actgccgatg aggtagttaa tcgcgccggg aagctggcgg cgaaatttcg cgccagcggt
     4801 cagcccgtgt ttctggtgcg cgttggctgg tctgccgatt acgccgaagc attaaaacag
     4861 ccggttgatg ccccttcccc cgctaaagtg ttgcccgaaa actggtggca acatccagct
     4921 gcattaggtg caactgacag tgatatcgaa ataatcaaac gtcagtgggg tgcgttttac
     4981 ggtacggatc tggagttgca attacgccgc cgaggtatcg atacaatagt gttatgtggg
     5041 atctcgacca atatcggagt tgaatccacc gcccgcaatg cctgggaact tggctttaat
     5101 ctggtgattg ccgaagatgc ctgtagtgcc gctagcgccg agcagcacaa taacagtatc
     5161 aaccatatct acccgcgcat cgcccgtgtg cgtagcgtgg aagagatcct caacgcgtta
     5221 tgatttacat cggtctaccg caatggtcgc atcctaaatg ggtgcggttg gggatcacca
     5281 gccttgaaga gtatgcccgt cactttaact gcgtggaggg caacaccacg ctttacgccc
     5341 tgccgaaacc cgaggttgtc ctgcgctggc gtgaacagac cacagatgac ttccgcttct
     5401 gttttaagtt tccggcgacc atttcgcatc aggcagcatt acgacattgc gatgatttag
     5461 tgactgaatt tttgacccgc atgtcaccgt tggctccgcg cattgggcaa tactggctgc
     5521 aactgcctgc cacattcggc ccacgggagc tgcctgcgct ttggcatttt ctcgattctc
     5581 tccctggcga atttaattat ggtgtggaag tccgccatcc acagtttttc gccaaagggg
     5641 aagaggaaca aacgcttaat cgcggtttac atcagcgcgg cgttaatcag gtgattttag
     5701 acagccgccc ggttcatgca gcacgtccac acagtgaagc tattcgcgac gctcaacgaa
     5761 aaaaacctaa agttccggta catgctgtac tgacggcgac aaatccgctg atccgtttta
     5821 tcggtagtga tgatatgacg caaaaccggg aattatttca ggtctggtta caaaaattag
     5881 cgcagtggca tcagaccact acgccttatc tttttttaca tacgccagat attgcccagg
     5941 ccccggaact ggtacatacc ctgtgggaag acttacgtaa aacgcttcca gagatcggag
     6001 cagttccggc tattccacag caatcttctc ttttctgaat ttgccaccta tcatagacag
     6061 gtgccatcgg ccattttaaa gggagtttgt atggtaagcg cgctgtatgc cgttttaagt
     6121 gcgttgttat taatgaagtt ctcttttgat gtcgttcgcc tgcgaatgca gtaccgcgtt
     6181 gcctatggtg acggtggttt tagcgaactg caaagcgcta ttcgcattca tggtaacgcg
     6241 gtggaatata ttcccatcgc gattgtgctg atgctgttta tggaaatgaa tggcgcagaa
     6301 acctggatgg tgcatatttg cggcatcgtt ttgcttgctg gtcgtctgat gcattattac
     6361 ggttttcatc accgtctgtt ccgctggcga cgttccggca tgagcgccac ctggtgtgcg
     6421 ctgttgctga tggtgctggc gaatctttgg tatatgccct gggagttggt tttctccctg
     6481 cgttagcgca caatacgcca ctttcttttt cccggatttt tacgttatgt ctcaccgcga
     6541 cacgctattt tctgccccta tcgccagact gggcgactgg acctttgatg aacgggtagc
     6601 tgaagtcttc ccggatatga tccagcgttc cgttcccggc tattccaata ttatttccat
     6661 gattggtatg ttagccgagc gcttcgttca acctggtacg caggtttacg atctgggttg
     6721 ttctctgggc gcggcgacgc tctcggtgcg tcgcaacatt catcatgata attgcaaaat
     6781 tattgccatc gacaactccc cggcgatgat tgaacgctgc cgtcgtcata ttgacgccta
     6841 taaagcccct acgccagtag acgtcattga aggtgatatt cgcgatatcg ccattgaaaa
     6901 cgcatcgatg gtggtgctga attttaccct gcaattcctg gaaccttccg agcgccaggc
     6961 gttactggat aaaatttatc aagggctgaa ccccggcggc gcgctggtgc tttcggaaaa
     7021 attcagtttc gaagatgcca aagttggtga actgttgttc aacatgcacc acgactttaa
     7081 acgtgccaac ggttacagcg aactggagat cagccagaag cgcagcatgc tggaaaacgt
     7141 gatgctgacc gattccgtgg aaacccataa agcacgcctg cataaagccg gttttgagca
     7201 tagcgagctg tggttccagt gctttaactt tggttcactg gtggcattaa aagcagagga
     7261 cgctgcatga tcgactttgg taacttttat tctctgattg ccaaaaatca tctttcacac
     7321 tggctcgaaa cgctgcccgc gcagattgct aactggcagc gcgagcagca gcacgggttg
     7381 tttaagcagt ggtcaaatgc ggtggagttt ctacctgaaa ttaaaccgta tcgtcttgat
     7441 ttattgcata gcgttacggc agaaagcgaa gagccactga gcgcagggca aattaaacgc
     7501 attgaaacgc tgatgcgcaa cctgatgccg tggcgcaaag ggccgttctc actgtatggc
     7561 gtcaacatcg ataccgaatg gcgttccgac tggaaatggg atcgtgttct gccccatctt
     7621 tctgatttaa ccggacgcac cattctggat gtcggctgcg gcagcggtta tcacatgtgg
     7681 cgcatgattg gcgcaggcgc gcacctcgcg gtggggatcg accctacgca actgtttctg
     7741 tgccagtttg aagcggtgcg taaactactg ggtaatgatc agcgcgcgca tttgttaccg
     7801 ttaggtattg aacaacttcc ggcactgaaa gcctttgata ccgtcttttc gatgggcgtg
     7861 ctttatcatc gtcgttcacc gctggagcat ctctggcagt tgaaagatca actggtcaat
     7921 gaaggtgaac tggtgcttga aacgctggtt attgatggcg atgagaacac ggtgctggtg
     7981 ccgggcgatc gttacgcgca aatgcgtaat gtctatttca tcccttccgc gctggcgctg
     8041 aaaaactggc tgaagaagtg tggttttgta gatatccgca ttgcggatgt gagcgttacc
     8101 actacagaag agcagcgacg cactgaatgg atggttaccg agtctctgtc cgatttcctc
     8161 gacccacatg atccgagtaa gacagttgaa ggttatcctg cgcctaaacg cgcggtgctg
     8221 attgcgcgca agccgtaaag gtctggtatt actgccggat gcggcgtgaa cgccttatcc
     8281 agcctacaaa accttgctaa ttcaatatat tgcagggact atgtaggcct gataagcata
     8341 gcgcatcagg cagctttatg tttgcataac ctcagcgccc gtttccgggc gcttttcacg
     8401 tcttatgcct gaacagctgg ctgatcaaac gccgttaact tcggtgcgtt acccgtatat
     8461 ttttcgatat acaccagcgc agagttaccg gcacaggcat ttgccagctg cgagctgggg
     8521 atatccgccg ttaacacgtt cgcactgccg tttttacaca agccattttc cagatctggc
     8581 catgcacctt catgcacgca taccacgcct tttttgatcc cgtcagtcac cacagcgcct
     8641 gtcaaaatct gaccacgttt gttccagaca cggaccagat cgccattcgc aataccaaag
     8701 cgagcagcat cttcggtgtg aatagtgatt ggttcacgat cggcaaccgc atatttttta
     8761 cgcagttgcg catagttaag ctgactgtgt aaacggtgtg ccggatgtgc ggtcagaagc
     8821 tgtaactgct tctcgtcggc ggtacctttc cactcatcag gcgcaagcca ggttgggtgt
     8881 gccgggcaat ccttatagcc aaatttttcc agcgttttgg agtaaatctc aattttgccg
     8941 cttggcgtac ccagcgcatt tttcaccgga tcggcgcgga aatcaccata acgaacgtac
     9001 tgttcgttct tctcgctgcg gcgcatttcg atcagtttat tttgctgcca gaaggcatta
     9061 aacattggca tagtgacgcg ttgcgcacgg gcacctttct gagcggcatc atagaaaaat
     9121 ttcagccacg ccatttcatc tttaccttcg gtatagatct cttttccgcc tggtttcagt
     9181 aattccgcaa gttcggcaaa cacgtcaaaa tcgtttcgcg cttcaaattg cggagcgaca
     9241 gcctgcttca tcggtacaat atgctggttg ctgtaatcac cggtcatcgt caagtcattg
     9301 cgctcaaacg atgtggtgat cggtaatacg atatctgcat gtttagcagc tgcggtccag
     9361 tagcattcag aaacgacgat catctccggt ttctgccatg ctttgatcag acgattagta
     9421 tcctggtgat gggtaaagtt accgccaccc gcccaccaga tcattttgat attcggatac
     9481 gtttgttctt taccgttatg ctgatatttg ccgccaggat tttccagcgc atcgacgata
     9541 cgggcaacag gaatcgccgt cattccgcca tcatcagcgg cctcgctggc ctgtccggca
     9601 atcgccgcag acatttccgg cagcacgccg ccaacacgcg tcgggttgcc accgttggag
     9661 taatgataag agaaaccaaa accgccgccc ggtgtaccaa tctgccctaa catcgccgcc
     9721 agcgtcacca gcatccagtg tttctgttca ccatattgct ggcgctgaat tccccagcct
     9781 gccatcagca ttgtgcgatt cgccgccatc agttcagcga gtttgactat ctgcgcttcc
     9841 ggtacgccag taatttccgc cgtccaggcc gcgctcttcg gagtattatc gctcttacct
     9901 gtcagatact cttcaaactg cggatacccg gtggtgtatt tttcaaggaa cactttatcg
     9961 tgtttgcctt gtgtcatcag ggtatgcgca atccctaaca tcagtgccac gtcggtgccc
    10021 atattcggcg cgatccaggt ggcattatag ccaaagaact cgatggtttc agagcggata
    10081 ggatcaatgg caatcactgg tttgccagat tttttcagct gatggaagta ttccagccct
    10141 tgctcatcgg tactgctcca ggcaattttt aaggtattca gcgggttcat tccccacagc
    10201 accacaacct ggctgttttc cagaatcagc ggccaggagg tctgctgttc atacacctct
    10261 acagaaccga ccacatgcgg catgatcacc tgtgctgccc cggttgaata atcgccgcta
    10321 tgcccggaat aaccgcccgc caggttcata taacgttgca gtaaggtttg cgctttatgc
    10381 aacacgccag aagagcgcca gccgtaagat ccggcaaaaa tggccgatgg tccgttagct
    10441 ttacgaatac gctcatgttg ctcatgaatc agttttaatg cctgttccca gctcacctgt
    10501 acataggtat cttcgccacg acctttcgcc ggttgcagtg gattgtcgag atagcttttt
    10561 ctcaccatcg gatgctgaat acgcgccgtg gtgtgtacct gatccgccgc cgtagactgt
    10621 aaggagttcg gtatggtttt cgccagcgcg cctgtcgaag aaacaatctt gccgtccttc
    10681 acttctacgt tcatcgctcc ccaacgtccc gcggtgagga ttttaccgcc cttctcttct
    10741 gcccatgcgg gtagcggtgc tgccgatgtc accaccagcg ctccagcggc aataccgctg
    10801 tgtttaataa attcacgtct tgttaatgtc ataacttcct ccctgatcaa cgaggatcac
    10861 tgtttctcgg taatatcttt ggcgttgtac tggaaatacc gcgttaaaat gtccagttcg
    10921 ttttcgctca tgctggttcg tgcccccatt cctttggcaa tggacggcca cgcattgacg
    10981 gtgtaatggt cggcggcaat aggggcatga caaccagcgc aataggtatc gtcaagtttt
    11041 tcagcgtatt gccatagcag tttacggtcc gctaatgcgg gatcggtaag cgcaccctgt
    11101 aaagacgcct gacgccattg attgccgtat tcgtcagcct gccattcccc gtttaccgtg
    11161 agcgccttga taccttcttc acttaatgtg gctagcgcca gccgttgacc tgccgccagg
    11221 tagagcgtgt tttcactgcc ctgcatttga taaccctgca acagaacgat cggctgtttg
    11281 ccactggcat caacgacggt gagatcggta ccaggattca cggtagccag ctcgcctatg
    11341 cgagaagttt tgaaaggata aatatgtgcg ccattagtca ctgaagtagc ggcctgactt
    11401 tccagctcat gcgccgcgtt gtcatccatt tttatttctg gcggaaaatg ggcaatgcct
    11461 ttatgacaat cgatacaggt ttcgccgccc ttttgtgctt tgttatgcat tttctgcgca
    11521 gattcacttt gcgaggcaat atccatggca tcaaaagaat ggcaactacg gcacgttgca
    11581 gagtcagttg cttttaattc tttccatact gtttcggcca tttcctggcg atgagcttcg
    11641 aacttatcgt cactgtctat tttgccgcta acaaattcat gataaatatc tttaga
//
LOCUS       DBFIFM010000141        11598 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.141, whole genome shotgun sequence.
ACCESSION   DBFIFM010000141 DBFIFM010000000
VERSION     DBFIFM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.141"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            326..1714
                     /gene="narU"
                     /locus_tag="N7C74_003362"
     CDS             326..1714
                     /gene="narU"
                     /locus_tag="N7C74_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HGU2433172.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWKNKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAR
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRMKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1796..5536
                     /gene="narZ"
                     /locus_tag="N7C74_003363"
     CDS             1796..5536
                     /gene="narZ"
                     /locus_tag="N7C74_003363"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HGU2433173.1"
                     /translation="MSKLLDRFRYFKQKGDTFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKSLSYKQVRGRGGFIR
                     SNWPELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLAGGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLSPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRICPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5533..7077
                     /gene="narH"
                     /locus_tag="N7C74_003364"
     CDS             5533..7077
                     /gene="narH"
                     /locus_tag="N7C74_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HGU2433174.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSIAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7077..7772
                     /gene="narW"
                     /locus_tag="N7C74_003365"
     CDS             7077..7772
                     /gene="narW"
                     /locus_tag="N7C74_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HGU2433175.1"
                     /translation="MQILKVIGLLMEYPDEMLWECKEDALALIRRDAPMLTDFTRNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7769..8449
                     /gene="narI"
                     /locus_tag="N7C74_003366"
     CDS             7769..8449
                     /gene="narI"
                     /locus_tag="N7C74_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HGU2433176.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8528..9421
                     /gene="yddE"
                     /locus_tag="N7C74_003367"
     CDS             8528..9421
                     /gene="yddE"
                     /locus_tag="N7C74_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HGU2433177.1"
                     /translation="MEPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHHDGYRISLEQGTPGFEPPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            complement(9517..10361)
                     /gene="nhoA"
                     /locus_tag="N7C74_003368"
                     /pseudo
     CDS             complement(9517..10361)
                     /gene="nhoA"
                     /locus_tag="N7C74_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
     gene            10660..11103
                     /locus_tag="N7C74_003369"
     CDS             10660..11103
                     /locus_tag="N7C74_003369"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HGU2433178.1"
                     /translation="MPVEFEPPRVTIVVDKSTWTRELIERNGKFGIVIPGVAATNWTW
                     AVGSVSGREEDKFNCYGIPVVRGPVFGLPLVEEKCLAWMECRLLPATSAQEEYDTLFG
                     EVVSAAADARVFVEGRWQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11107..11334)
                     /gene="pptA"
                     /locus_tag="N7C74_003370"
     CDS             complement(11107..11334)
                     /gene="pptA"
                     /locus_tag="N7C74_003370"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HGU2433179.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11433..>11598)
                     /locus_tag="N7C74_003371"
     CDS             complement(11433..>11598)
                     /locus_tag="N7C74_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HGU2433180.1"
                     /translation="RRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYL
                     ASVLAGSGLS"
BASE COUNT         2733 a         3013 c         3039 g         2813 t
ORIGIN      
        1 catggccttt ttatcactaa ttctataagt gtctggtctt gttaaaaata tagttaatat
       61 acaacccttt ggattcatca tctgaagggt tgaatcatga catgcgtaca tagcttaacc
      121 ccgtttaatg gttagcttgt tagttacatt tagtaacaca taactggctg cttaatcccc
      181 attctgcccc cgcggttacg ctttcttatc tattctttca atcagcactt cacccatttc
      241 atccttaatt ctaataactg gctattgcgc cttacctcct gttttgtctt tttttcatta
      301 ccaatgtgta catgtgagga acaatatggc actgcaaaat gagaaaaata gtcgttatct
      361 tttacgcgac tggaaaccag aaaatccggc cttctggaaa aataaaggaa aacatattgc
      421 tcgaagaaac ctctggatat cagtcagttg tctacttctt gccttctgtg tctggatgct
      481 atttagcgca gttaccgtta atctcaataa aatcggtttt aatttcacta ccgatcaact
      541 ctttttatta accgcattac cctccgtttc tggcgcatta ttacgtgtcc cctactcctt
      601 tatggtgcct atattcggtg gacgtcgatg gacggttttt agtactgcaa tcctgattat
      661 tccttgcgtc tggctcggaa ttgccgtgca gaatccgaat actccttttg ggatatttat
      721 cgttatcgct ttgctatgcg gttttgcagg tgcgaacttt gcttcgagca tgggcaatat
      781 cagtttcttc tttccaaaag ccaggcaagg tagcgctctt gggattaatg gcggattagg
      841 aaacttaggt gtaagtgtaa tgcagctggt tgcaccgctg gtcatttttg tacccgtatt
      901 tgcctttctc ggcgtcaatg gagtaccgca ggccgacggt tcggtgatgt cgctggcgaa
      961 tgccgcatgg atttgggtac cgctactagc gattgccacg atcgccgcct ggtcagggat
     1021 gaatgatatc gccagttcac gcgcgtcaat tgccgaccag ctccctgtct tacaacgcct
     1081 gcatctctgg ctgctgagcc tgctttacct tgccaccttc ggttcgttta tcggtttttc
     1141 tgcgggtttc gccatgctgg caaaaaccca gttcccggat gtgaatattc tgcgcctggc
     1201 gttctttggc ccatttatcg gtgccatcgc gcggtcggtg ggtggtgcta tttccgataa
     1261 attcggcggc gtgcgggtga cgttgatcaa ctttattttt atggcgattt tcagcgccct
     1321 gctgttcctt accttaccgg gcacaggctc cggtaacttc atcgcctttt acgccgtatt
     1381 tatggggctg tttctgaccg cgggtctggg aagtggttct actttccaga tgatcgccgt
     1441 catctttcgc cagataacca tttatcggat gaagatgaaa ggcggtagtg atgagcaagc
     1501 tcagagagaa gccgtcaccg aaacggcggc ggctctgggc tttatctcag ccatcggcgc
     1561 agtgggcggc ttttttattc cgcaggcatt tggcatgtca ctcaatatga ccggctctcc
     1621 agtgggcgcg atgaaagtgt ttttaatctt ctacatcgtt tgtgtgctac tgacctggct
     1681 ggtttatggt cggcggaagt tcagccaaaa ataagagatc gatgcctgat gtacaaccgt
     1741 gtcgcatccg acaaggcatc agatcccaat tcattcctgg agcaggagtc atgtcatgag
     1801 taaacttttg gatcgctttc gctacttcaa acaaaagggc gatacctttg ccgatggtca
     1861 cggacaggtg atgcatagca accgcgactg ggaggacagc tatcgccagc gttggcagtt
     1921 cgacaaaatc gtgcgttcca cccacggtgt taactgtaca ggctcctgta gctggaaaat
     1981 ctacgttaaa aatggtctgg tgacctggga aatccaacag accgactacc cgcgcactcg
     2041 ccctgacctg cccaatcatg aacctcgcgg ctgcccgcgt ggcgcaagtt actcctggta
     2101 tctttacagc gctaaccgcc tgaaataccc gctcattcgt aaacgactga ttgaactgtg
     2161 gcgcgaagcc ctcaaacaac acagcgatcc ggtactggcg tgggcatcga ttatgaacga
     2221 tccgcaaaag agcctgagct acaaacaagt gcgtgggcgc ggcgggttta tccgctccaa
     2281 ctggccggaa ctaaaccagc tgattgccgc cgctaacgtc tggactatca aaacctacgg
     2341 cccggatcgc gttgccggtt tctcgccgat cccggcgatg tcgatggttt cttacgccgc
     2401 cggaacacgt tatctgtcac tattaggtgg cacctgttta agcttctacg actggtattg
     2461 cgacctgccg cccgcctcgc cgatgacctg gggcgagcaa accgacgtac cggaatctgc
     2521 cgactggtat aactccagtt acatcatcgc gtgggggtct aacgtaccgc agacacgtac
     2581 gccggacgcc catttcttta ccgaagtacg ctacaaaggc actaaaacca tcgccattac
     2641 ccctgactac tctgaagtgg ccaaattgtg cgaccagtgg ctggcaccaa aacaaggcac
     2701 tgatagcgcc ctggcgatgg caatgggcca tgtgatttta aaagagtttc atctcgataa
     2761 tcccagcgac tactttatca actactgccg ccgctatagc gacatgccga tgctggtaat
     2821 gctggaacct cgcgacgatg gtagctacgt acccgggcgc atgatccgcg cgtctgacct
     2881 ggtagatgga ctaggcgaaa gcaataatcc gcagtggaaa accgtggcgg ttaataccgc
     2941 aggtgaattg gtagtgccga atggttcgat tggtttccgc tggggagaaa aaggcaaatg
     3001 gaatctggaa tccattgccg ccggtaagga aaccgaattg tcgttaaccc tgctcggtca
     3061 acatgacgcc gttgcaggcg tggctttccc ctactttggc ggcatcgaaa atccgcattt
     3121 tcgcagcgtc aaacacaatc ctgtcctggt gcgtcaatta cccgttaaaa acctgacatt
     3181 agccggtggc agcacctgtc cagtggtcag cgtttatgat ttggtactgg cgaattacgg
     3241 cctcgatcgc gggctggaag atgaaaacag cgcgaaagat tacgctgaaa tcaaaccgta
     3301 caccccagcc tggggtgagc aaattaccgg cgtgccgcgc cagtatattg aaaccatcgc
     3361 tcgtgaattt gccgatactg cccataaaac gcatgggcgc tcgatgatta tcctcggcgc
     3421 aggtgttaac cactggtatc acatggacat gaactaccgg ggaatgatca atatgctgat
     3481 cttctgcggt tgtgtcggac aaagcggtgg cggctgggca cactatgtcg gtcaggaaaa
     3541 actgcgcccg caaaccggct ggttgccgct ggcctttgcg ctcgactgga accgaccacc
     3601 gcgccagatg aacagcacct cgtttttcta caatcattcc agccaatggc gctatgaaaa
     3661 agtctctgcg caggagttac tttcaccgct tgccgatgcc agtaagtaca gcggtcatct
     3721 gattgatttt aacgttcgcg ccgaacgcat gggctggcta ccttctgcgc cgcagttggg
     3781 gcgtaacccg ctcgggatta aagctgaagc cgacaaggcc ggattatccc ccacagaatt
     3841 taccgcccag gcgctgaaat cgggcgattt acgtatggcc tgcgaacaac cagatagcgg
     3901 cagcaatcat ccgcgtaatt tgtttgtctg gcgttctaac ctgcttggct cctccggcaa
     3961 aggccacgag tatatgcaga agtatctgct ggggaccgaa agcgggattc agggcgagga
     4021 actcggtgcc agcgacggga tcaaaccgga agaagtcgag tggcaaactg cggcgattga
     4081 aggcaagctc gacctgctgg tgacgctcga cttccgcatg tccagtacct gcctgttctc
     4141 cgatatcgtt ctgcccaccg ccacctggta tgaaaaagac gatatgaaca cctcggatat
     4201 gcatccattt attcatccgc tttctgcggc ggtcgatccg gcctgggaat cacgcagcga
     4261 ctgggaaatc tacaaaggta ttgccaaagc attttcgcaa gtgtgcgtgg gccatcttgg
     4321 caaagaaacc gacgtggtat tacaaccact gctgcatgac tctccggcag agctctcaca
     4381 gccgtgtgaa gtgctcgact ggcgcaaagg cgaatgcgat ctgagcccgg gtaaaaccgc
     4441 gccgaatatt gtggcggtgg agcgcgacta ccctgctacg tatgaacgct ttacctcgct
     4501 cgggccattg atggacaaac ttggcaacgg cggtaaaggg atttcgtgga atacgcagga
     4561 tgaaatcgat ttcctcggta aactcaatta caccaagcgt gatggcccag cgcaggggcg
     4621 tccgctgatt gacaccgcca ttgacgcttc agaagtgatt ctggcactgg caccagaaac
     4681 caacggtcat gttgcagtta aagcgtggca ggcgctgggc gagatcaccg gacgcgaaca
     4741 tacccatctg gcgctgcaca aagaggacga gaagattcgc tttcgcgata ttcaggcgca
     4801 gccgcgtaaa attatctcca gccccacatg gtctggtctg gaaagcgatc acgtctccta
     4861 taatgcggga tacaccaacg ttcatgagtt aattccgtgg cgcacgctgt cgggacgcca
     4921 gcagctctat caggatcatc cgtggatgcg tgcttttggt gaaagcctgg tggcatatcg
     4981 cccgcctatc gacacccgta gcgtcagtga gatgcgccag atcccgccaa acggcttccc
     5041 ggaaaaagca cttaacttcc tgacgccgca ccagaaatgg ggcattcact caacctacag
     5101 tgaaaacctg ctaatgctga cgctctctcg cggtggaccg attgtctgga tcagcgaaac
     5161 cgatgcccgt gaactaacca ttgtcgataa cgactgggtg gaagtgttta acgccaatgg
     5221 cgcgctgacg gcccgcgcgg tggtcagcca acgtgtaccg ccgggtatga ccatgatgta
     5281 tcacgctcag gaacgcatta tgaatattcc tggttcggaa gtaactggca tgcgcggcgg
     5341 tattcataac tcggtcaccc gcatttgccc gaaaccaacg catatgattg gcggttacgc
     5401 gcagctggcc tggggcttta actactacgg caccgtcggc tcgaaccgcg acgaattcat
     5461 catgatccgc aagatgaaga acgttaactg gctggatgat gaaggtcgcg atcaggtaca
     5521 ggaggcgaaa aaatgaaaat ccgttcgcaa gtcggcatgg tgcttaatct cgataaatgt
     5581 atcggctgcc atacctgttc ggtgacctgt aaaaacgtct ggaccggacg cgaaggcatg
     5641 gagtacgcat ggtttaacaa cgtcgaaacc aaaccgggca ttggttatcc gaaaaactgg
     5701 gaagatcagg aagagtggca aggcggctgg gtccgcgatg tgaatggcaa gatacgcccg
     5761 cgtctgggca gcaagatggg tgtaataacc aaaatcttcg ccaacccggt ggtgccgcag
     5821 attgatgatt actacgaacc tttcaccttc gactacgaac atttgcatag cgcaccggaa
     5881 ggcaaacata tccctactgc tcgcccgcgt tcgctgattg acggtaagcg gatggacaaa
     5941 gtgatctggg ggccaaactg ggaagaactg ctgggcggcg agtttgaaaa acgtgcccgc
     6001 gaccgcaact tcgaggccat gcaaaaggag atgtacgggc agtttgaaaa caccttcatg
     6061 atgtacctgc cgcgcctgtg cgaacactgc ctcaatccca gctgcgtggc gacctgccca
     6121 agcggcgcta tctacaaacg cgaagaagac ggcattgtgc tgattgatca ggataaatgc
     6181 cgtggctggc gtttgtgcat tagcggttgt ccgtacaaaa aaatctactt caactggaaa
     6241 agcggcaagt cagaaaaatg tatcttctgt tacccacgaa ttgagtccgg tcaaccgacc
     6301 gtgtgctcag aaacctgcgt gggtcgcatc cgatatctgg gcgtgctgct ttacgacgct
     6361 gaccgcatcg aggaagcggc gagcaccgag cgcgaggttg acctctatga acgccagtgc
     6421 gaagtgttcc tcgatccgca cgatccctca gtgatcgagg aagccctgaa acaaggtatt
     6481 ccacaaaatg tgattgacgc tgcccagcgt tcgccagtct acaaaatggc gatggactgg
     6541 aaactggcac taccgctgca ccctgaatac cgcaccctgc cgatggtctg gtacgttcct
     6601 ccgctgtcac cgattcagtc ctacgcagat gcgggcggtt tgccgaaaag cgaaggcgtg
     6661 ctgcccgcca tcgaaagcct gcgtattccg gtgcaatatc tcgccaatat gttgagtgcc
     6721 ggcgataccg gtccggtact gcgggcgctg aaacggatga tggcgatgcg ccactatatg
     6781 cgttcacaaa ccgtggaagg cgttactgat actcgtgcca tcgacgaagt aggcctgagc
     6841 atcgcccagg tcgaagaaat gtatcgctat ctcgccattg ccaactatga agatcgtttt
     6901 gttatcccga ccagccatcg ggaaatggcg ggcgatgcct tcgcagaacg caatggctgc
     6961 ggttttacct ttggtgacgg ttgtcacggc tcggacagca aattcaacct gttcaacagt
     7021 agccgtatcg atgccatcaa catcaccgaa gtgcgcgaca aagcggaggg cgaataatgc
     7081 agatcctcaa agtgatcggc ctgttgatgg agtatccgga cgagatgttg tgggaatgca
     7141 aggaggacgc gctggcgttg atccgccgcg atgcgccgat gcttacggat ttcacccgca
     7201 acctgcttaa cgcgccgctg ctggataaac aggccgaatg gtgcgaagtg tttgaccgcg
     7261 ggcgcaccac gtcgctgctg ctgttcgaac atgttcatgc cgagtcccgc gatcgcggcc
     7321 aggcaatggt ggacctgctg gcggagtatg aaaaggtcgg cctgcagctg gattgtcggg
     7381 aactgcccga ttatctaccg ctgtatctgg agtatttaag cgtactgcct gacgatcagg
     7441 cgaaagaagg attgctcaac gttgcgccga tcctcgccct gcttggcggt cgcttaaaac
     7501 aacgcgaggc accgtggtac gcgttgtttg atgctctgct gcaactggcg ggaagtacac
     7561 tttcaagtga cagtgtcaca aaacaagtaa acagcgaaga gcgcgatgac acccgccagg
     7621 cgcttgatgc agtgtgggaa gaggaacagg tgaagtttat tgaagataac gccacggcgt
     7681 gtgacagctc gccgcttaat caatatcagc gacgctttag tcaggatgtc gcgccgcaat
     7741 atgtcgacat cagtgcggga ggtgggaaat gattcagtat ctgaacgtct ttttttacga
     7801 tatctacccg tacatttgtg cgacggtgtt tttcctcggc agctggctgc gctacgacta
     7861 cgggcagtac acctggcgcg cctcctcaag tcagatgctc gataaacgcg ggatggtgat
     7921 atggtcgaat ctgttccata tcggcatttt ggggatcttc ttcgggcacc tgttcggcat
     7981 gttaacgcca cactggatgt acgcgtggtt tttgccagtg gcagcgaaac aactaatggc
     8041 gatggtgctc ggtggtattt gcggtgtgtt gacgttgatt ggcggcgcag gactgctgtg
     8101 gcgcaggctg acaaatcagc gcgtgcgtgc cacttccact acgccggata tcatcatcat
     8161 gagcattttg ctgatccagt gtctgttggg gctaagtacc ataccgttct cagcacagta
     8221 tcctgatggt agcgaaatga tgaagctggt gggttgggcg caaagcattg tcactttccg
     8281 tggtggatcg tcagagatgc ttaacggtgt agcgttcgtc ttccgcctgc atctggtgtt
     8341 gggaatgacc atcttcttgc tcttcccatt cacccgactg gtgcatgtgt ggagcgcccc
     8401 gtttgagtac tttactcgtc ggtatcagat tgtgcgctcg cggcgataat ttcaccctgg
     8461 ccggatagcc cttcgctctc cggccacttg caggctatga tagttctcca gtcacaggag
     8521 aacgttaatg gaaccgcagg tttaccacgt cgatgctttt acctctcaac catttcgcgg
     8581 caattctgcc ggtgttgttt tccccgccga taatcttagc gaagcgcaaa tgcagcttat
     8641 cgcccgcgaa ttaggtcatt cggaaaccgc ttttctgctg cacagcgacg acagcgatgt
     8701 gcgcatccgc tactttacgc caacagttga agtgcctatt tgcggtcacg cgacagtagc
     8761 tgcgcactat gtacgtgcga aggtgttagg tttaggaaat tgcacggtct ggcaaacatc
     8821 gctggcagga aaacatcgcg tgactatcga aaagcaccac gatggttatc gtatttcgct
     8881 ggaacaaggt acgccaggct ttgagccacc gctggaaggt gaaacacgtg cggcgattat
     8941 caacgcgctt catctcactg aggacgatat tctgccaggc ttgccaattc aggtggcaac
     9001 cacaggacac tcaaaagtga tgatcccact gaaaccggaa gtggatatcg acgccctttc
     9061 gcctgacctt aatgcgctga ccgctatcag caaacagatt ggctgcaatg gtttcttccc
     9121 gttccagatc cgcccaggca aaaacgaaac cgatggtcgc atgttctcgc ctgcgattgg
     9181 tattgtggaa gatccggtga ctggcaatgc caatggcccg atgggtgcat ggttggtaca
     9241 tcacaacgta ttgccccacg atggcaacgt gttgcgtgtt aaaggccatc aggggcgcgc
     9301 attggggcgt gacggtatga ttgaagtgac ggtgacaatt cgtgataacc aaccggaaaa
     9361 agtcaccatt tctggcgcgg cggtgatttt gtttcatgcc gaatgggcaa ttaaactctg
     9421 aaaccgttgc ctgatgcgct tcgcctgtca ggtctacaac cgggctaatg tatgccggat
     9481 aaggcgttta cgccgcatcc ggcaacctga cataaattat tttcccgcct ccgggtgcgt
     9541 atcaaacgcc gccatcacca gcgctaactc atccacggta aagccatgtt tcgcatcatc
     9601 cacgcccaga ccaaactgtt cttgcatcac agcatataac gacgccacat ccggtagatt
     9661 tcgctgctcc accgcgtgcc cattttcata atgggaaaat gaaaattggt cagtgtcagc
     9721 ttgccgccgt ccggcaaatg gcggcacatc agcaaatgat ggcgaaaatg cgactgcggc
     9781 cagtgcgccg accagaaatt acccatcaca taatcgcttt gttgctgctc gcagagatca
     9841 aaacggtaca tcgactgcca gtgctgatga tgattaaact gcaacaccca atcatcaccc
     9901 tcctgcaaca accgatactc tccgtgtggc gtggtctgca caagatcggg aactaaacga
     9961 atcggcgcgg ttagcgtctg cccaccgaaa ccgacatcag caatccattt ttcctcttcc
    10021 agttccacca acagcaaacg atgggtgcgc ggcggtaatg ctggcggatt tgataacact
    10081 acccgcccta acaagctgcg aacgttaaac cccagctcgc gtaacacccg ctcaaaaacg
    10141 ccattctgct caaaacagta accgccacga cgggctatca ccagtttctc ttccagcgat
    10201 tgatcatcaa gctgcatttc cctcggcagc aaaacgtcga ggttttcaaa cggaatggtg
    10261 caattgtgtt tcaggtgcaa tgcacgaagc gtatcaatat tgaccgcagc agctcccgac
    10321 cagttaatac gggcaaaata gtgattcaga atgggcgtca tcaggcgttt cccttagtgg
    10381 ttttctcaac ttataacctg gaatagacgc cgcaccggct attttgtgcg atcgcggaca
    10441 tgcatcacgt tcgcaaatcg ccaatcccgt taatttcgtt acttattgcg ttacggacta
    10501 cgcttaaaaa aaccttcttc gcgaggcgat accatgagcc gattcatccc catagaatta
    10561 caccatgcca gccggctgtt aaatcatggc ccaactgtca tgatcaccag tttcgatgag
    10621 caatcccagc ggcgtaacat tatggctgca gcctgatcaa tgccggtgga gtttgaacct
    10681 ccacgcgtga cgattgtggt agataaatcg acatggacca gggagttgat tgaacgtaac
    10741 ggtaaatttg gcatcgttat cccgggcgtt gcagcaacta actggacgtg ggcagtggga
    10801 agtgtgtcgg gtcgtgaaga agataaattt aattgctatg gcattccggt tgtgagaggc
    10861 ccggtatttg gtttgcctct ggtcgaggaa aaatgtctgg cgtggatgga gtgtcgattg
    10921 ctacctgcga cttctgcgca agaagaatac gacacgctgt ttggcgaagt agtatcagca
    10981 gcggcagacg cacgggtatt tgtcgaaggc cgctggcagt ttgatgatga taagctcaat
    11041 acgttgcatc atttaggtgc tgggacgttt gttaccagcg gcaagcgtgt tacggcgggt
    11101 taagcattac atgctgtaac cgggtttctt tatcaaagcc tccatttggg gcgcgatttc
    11161 ggcatcccag atagcttgcc aagattctgg ttgaatctgt tgtagagcaa tgcttatcga
    11221 actgtcttta ctgttcagat ggcgaataat aacgtcggta atatctgcag caagtgctgc
    11281 tttttgttgt tcgtccagtt cacgcggaaa acatttaatg tctatgtgcg gcatgattac
    11341 gctccttttg aaagatgtca gtaaaattaa cacacattgt gaatgtcgtg tgtccggtaa
    11401 agcgttttta cgacaataat gccagggcaa gattacgaaa gcccgctccc cgcaaggact
    11461 gacgccagat agtttctgtc catggctgct tttcgcatct tacgtcttaa ccctgccttg
    11521 aataccttat cattcgtcaa aatattaata gcaatgtgcc gtatccctga aaataattct
    11581 gctgcatttc ctcttctt
//
LOCUS       DBFIFM010000142        11422 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.142, whole genome shotgun sequence.
ACCESSION   DBFIFM010000142 DBFIFM010000000
VERSION     DBFIFM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.142"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            294..1160
                     /gene="folD"
                     /locus_tag="N7C74_003372"
     CDS             294..1160
                     /gene="folD"
                     /locus_tag="N7C74_003372"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HGU2433181.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1162..1374
                     /gene="ybcJ"
                     /locus_tag="N7C74_003373"
     CDS             1162..1374
                     /gene="ybcJ"
                     /locus_tag="N7C74_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HGU2433182.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1482..2003
                     /locus_tag="N7C74_003374"
     CDS             1482..2003
                     /locus_tag="N7C74_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU2433183.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FNFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(2039..3424)
                     /gene="cysS"
                     /locus_tag="N7C74_003375"
     CDS             complement(2039..3424)
                     /gene="cysS"
                     /locus_tag="N7C74_003375"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HGU2433184.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKVEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3598..4092
                     /gene="ppiB"
                     /locus_tag="N7C74_003376"
     CDS             3598..4092
                     /gene="ppiB"
                     /locus_tag="N7C74_003376"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HGU2433185.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4095..4817
                     /gene="lpxH"
                     /locus_tag="N7C74_003377"
     CDS             4095..4817
                     /gene="lpxH"
                     /locus_tag="N7C74_003377"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HGU2433186.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRTKVHKPWLQTLFLALPLFVRKRIAVRMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4935..5444
                     /gene="purE"
                     /locus_tag="N7C74_003378"
     CDS             4935..5444
                     /gene="purE"
                     /locus_tag="N7C74_003378"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HGU2433187.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5441..6508
                     /gene="purK"
                     /locus_tag="N7C74_003379"
     CDS             5441..6508
                     /gene="purK"
                     /locus_tag="N7C74_003379"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HGU2433188.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARCFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLSATLEA
                     LIPLLPPEYASGVMWAQSKFC"
     gene            complement(6647..7540)
                     /locus_tag="N7C74_003380"
     CDS             complement(6647..7540)
                     /locus_tag="N7C74_003380"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGU2433189.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVRTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTDDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGMPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7537..8352)
                     /locus_tag="N7C74_003381"
     CDS             complement(7537..8352)
                     /locus_tag="N7C74_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HGU2433190.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8363..9622)
                     /locus_tag="N7C74_003382"
     CDS             complement(8363..9622)
                     /locus_tag="N7C74_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HGU2433191.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     TGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGASGMEAAR
                     AVSEEMAEIYLERNMQLQIPSWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9632..11299)
                     /locus_tag="N7C74_003383"
     CDS             complement(9632..11299)
                     /locus_tag="N7C74_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HGU2433192.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIMPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDAHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPTLRNQLITDLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPAAQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
BASE COUNT         2558 a         3247 c         3009 g         2608 t
ORIGIN      
        1 aatatagacc tgaagcggta aattaccagg ctgaaaattc tttttatatt gtcaggtatt
       61 tcttaaatta tcttaatcct taaacaagga aataaatcag ttccagattt acaacgccat
      121 catggacgaa aaatgaagct ttcaatctca gcgacctcag cgacagtgcg cctaaccttc
      181 gcaagaggtc gcttcacgcg ataaatctga aacgaaacct gacagcgcgc cccgcttctg
      241 acaaaatagg cgcatcccct tcgacctacg taacagatgg aatcctctct ctgatggcag
      301 caaagattat tgacggtaaa acgattgcgc agcaggtgcg ctctgaagtt gctcaaaaag
      361 ttcaggcgcg tattgcagcc ggactgcggg caccaggact ggccgttgtg ctggtgggta
      421 gtaaccctgc atcgcaaatt tatgtcgcaa gcaaacgtaa ggcttgtgaa gaagtcgggt
      481 tcgtctcccg ctcttatgac ctcccggaaa ccaccagcga agcggagctg ctggagctta
      541 tcgatgcgct gaatgccgac aacaccatcg atggcattct ggttcaactg ccgttaccgg
      601 cgggtattga taacgtcaaa gtgctggaac gtatacaccc ggacaaagac gtagacggtt
      661 tccatcctta taacgtcggt cgtctgtgcc agcgcgcgcc gcgtctgcgt ccctgcaccc
      721 cgcgcggtat tgtcacgctg cttgagcgtt acaacattga taccttcggc ctcaacgccg
      781 tggtgattgg cgcatcgaat atcgttggcc gcccgatgag catggaactg ttgctcgcag
      841 gttgcaccac caccgtgact caccgtttca ctaaaaatct gcgtcaccac gtagaaaatg
      901 ccgatctgtt gatcgttgcc gtcggcaagc cgggctttat tcctggtgac tggatcaaag
      961 aaggcgcaat tgtgattgat gtcggcatca accgtctgga aaatggcaaa gttgtgggcg
     1021 acgtcgtgtt tgaagacgcg gctaaacgcg cctcatacat tacgcctgtt ccgggcggcg
     1081 ttggcccgat gacggttgcc acgctgattg aaaacacgct acaggcgtgc gttgaatatc
     1141 atgatccaca gggtgagtaa catggcgaca ttttctttag gtaaacatcc gcacgttgag
     1201 ctgtgcgact tgctgaaact ggaaggctgg agcgaaagcg gcgcgcaggc gaaaatcgcg
     1261 attgccgaag gccaggtgaa agtcgacggc gcggttgaaa cgcgcaaacg ctgcaaaatc
     1321 gtcgccgggc agacagtgag ttttgcaggt cacagcgtac aggttgttgc ctgatccctt
     1381 tcccggcctg tatcaacagg ccggttctct tttcagactt ttgccctcta cgccccgttc
     1441 atccatgctg atagcatcga cattttgccg aagcgaccat catgccaacc gttattacgc
     1501 acgccgccgt tcccctatgc attggtttag ggttgggttc gaaagtcatc cctccgcgcc
     1561 tgttatttgc cggaatcatc ctggcgatgc tgcccgatgc cgacgtcttg tcgtttaatt
     1621 ttggcgttgc ttacggcaat gtttttggtc atcgcgggtt tacccattcg ctggtgtttg
     1681 cgtttgttgt cccgctgtta tgtgtgctga ttggacgtcg atggttcagg gctgggctga
     1741 ttcgctgctg gctgttttta accgtctcgt tgctatcgca cagcttgctg gattcggtaa
     1801 ccactggcgg taaaggggtt ggctggctgt ggccgtggtc agatgaacgc tttttcgctc
     1861 cctggcaggt gattaaagtc gcgccgtttg cattgtctcg ttacaccaca gcgtacgggc
     1921 atcaggtgat tatttctgaa ttgatgtggg tgtggttgcc ggggatgcta ctgatgggaa
     1981 tgttgtggtg gcgcagacga taaccggatg cgaaaactcg catccggcaa tagcgcaatt
     2041 acttacgacg ccaggtggtc ccttgcgggc catcttccag cacgatcccc atctcattaa
     2101 gacggtcacg cgccgcatct gccgccgccc agtctttcgc tttacgggca tccagacgct
     2161 gttgaattaa cgcttcaatc tcagccactt cgctgtcgtc tgcctgcgcg ccgctttgca
     2221 ggaacgcttc cggttcttgc tccagcaggc ccaatacggc ggaaagttta cgcaggtgag
     2281 atgccattgc attcgccgct gccatatctt ctactttcag acggtttact tcacgcgcca
     2341 tatcaaacag cacggaatag gcttccgggg tgttgaaatc gtcgtccatc gcttcaataa
     2401 agcgcgcttc aaacgcttcg ccaccggcag gcgcaacggt tttatctgtg ccgcgcagcg
     2461 cagtgtagag acgctccagc gccgcacgcg cctgcttcag gttctcttcg ctatagttca
     2521 gctggctgcg atagtggccc gacatcagga agtaacgcac ggtttccgcg tcgtagtatt
     2581 tcagcacatc gcgcacggta aagaagttac ccagcgattt ggacatcttc tcgcggtcaa
     2641 ccatcaccat ccccgagtgc atccagtagt tcacatactg accatcatgg gcacaggtgg
     2701 actgcgcgat ttcgttttcg tggtgcggga acatcaggtc tgaaccgccg ccgtggatat
     2761 caaagtggtt acccagctgc ttgcagttca ttgccgaaca ttcaatgtgc cagccaggac
     2821 gacccgcgcc ccacggagac ggccagctcg gttcgccctc tttcgacatc ttccacagaa
     2881 cgaagtccat tgggttgcgt ttgtcgtcga ccacgtcaac gcgcgcgcct gcctgcagct
     2941 ggtcgagatc ctgacgcgac agcacgccat aagttggatc ggtcgggacg tcgaacatca
     3001 cgtcgccgtt gtccgccaca taagcgtgac ctttggcgat cagttgttca gtgagttcaa
     3061 taatttctgc gatatggtgc gtcgcgcgcg gctccatatc cgggcgcaga atgttcaacg
     3121 catcaaaatc tttgtgcatt tcggcgatca tgcgatccac cagcgccaca aagctttcgc
     3181 cattttcatt ggcgcgtttg atgattttgt cgtcgatatc ggtaatgttg cgcacatact
     3241 tcagcttata gccgaggaaa cgcagatagc gcgcaaccac gtcaaaagca acaaaggtac
     3301 gcccgtgacc gatatgacag agatcgtaaa cggtgattcc acacacgtac atgccgactt
     3361 ccccggcgtg aataggctta aattcctctt tttggcgtgt cagagtattg aagattttta
     3421 gcatcgaaga ttccgtttag acatgtgtgg gtaattgagt tgcgtataat acccatattt
     3481 cccgcgcgaa tcagcataca ttgcgtgatg atccgatcgt gcggttatgc tataacacca
     3541 ccctatatat gacccgaact gggttgaagc accaatcaaa cggaacagga tgcaaaaatg
     3601 gttactttcc acaccaatca cggcgatatt gtcatcaaaa cttttgacga taaagcacct
     3661 gaaacagtta aaaacttcct ggactactgc cgcgaaggtt tttacaacaa caccattttc
     3721 caccgtgtta tcaacggctt tatgattcag ggcggcggtt ttgaaccggg catgaaacaa
     3781 aaagccacca aagaaccgat caaaaacgaa gccaacaacg gcctgaaaaa tacccgtggt
     3841 acgctggcaa tggcccgtac tcaggctccg cactctgcaa ctgcacagtt cttcatcaac
     3901 gtggttgata acgacttcct gaacttctct ggcgaaagcc tgcaaggttg gggctactgc
     3961 gtgtttgctg aagtggttga aggcatggac gtggtagaca aaatcaaagg tgttgcaacc
     4021 ggtcgtagcg gtatgcacca ggacgtgcca aaagaagacg ttatcattga aagcgtgacc
     4081 gttagcgagt aatcgtggcg acactcttta ttgcagatct tcatctctgc gtggaagaac
     4141 cggcgatcac cgccggtttt ctgcgttttt tagcgggaga agcccgcaag gccgacgcgc
     4201 tgtatattct tggcgatctg tttgaagcat ggattggcga cgacgatccc aacccactcc
     4261 atcgccagat ggcggcagcg atcaaagcgg tgtccgattc cggcgttccc tgttatttca
     4321 ttcatggcaa ccgtgatttt ctgctcggca aacgctttgc ccgtgaaagt ggcatgacgt
     4381 tattgccgga agaaaaagtg ctcgaacttt atggtcgtcg ggtgctgatt atgcatggcg
     4441 acacgctgtg caccgatgac gcgggttatc aggcttttcg caccaaagtc cacaaaccct
     4501 ggctgcaaac gctattcctc gctctgccgt tgtttgtgcg caaacgcatt gccgtgcgaa
     4561 tgcgcgcgaa cagtaaagaa gccaacagca gtaaatcgct ggcgatcatg gacgttaacc
     4621 aaaacgcggt agtcagtgcg atggaaaaac atcaggtgca atggctgatc cacgggcata
     4681 cccatcgccc ggcggtgcat gaacttatcg ccaatcagca acctgctttt cgcgtggtac
     4741 tgggtgcctg gcatacggaa ggttcgatgg tgaaagtcac ggcggatgac gttgagctga
     4801 ttcattttcc gttttaaaaa acccgcaact ttgctgattt cacagccacg caaccgtttt
     4861 ccttgctctc tttccgtgct attctctgtg ccctctaaag ccgagagttg tgcaccacag
     4921 gagttttaag acgcatgtct tcccgcaata atccggcgcg tgtcgccatc gtgatggggt
     4981 ccaaaagcga ctgggctacc atgcagttcg ccgccgaaat cttcgaaatc ctgaatgtcc
     5041 cgcaccacgt tgaagtggtt tctgctcacc gcacccccga taaactattc agcttcgccg
     5101 aaagtgccga agagaacggt tatcaggtga ttattgcggg cgcaggcggc gcagcgcacc
     5161 tgccaggcat gattgccgcc aaaacgctgg tgccagtgct gggcgtgcca gtacagagcg
     5221 ccgcactgag cggtgtcgat agcctctact ccatcgtaca aatgccacgc ggcattccgg
     5281 tgggtacgct ggcgattggt aaagctggcg cggcaaacgc ggcattactg gcagcacaaa
     5341 ttcttgcgac tcatgataaa gaactgcacc agcgtctgaa tgactggcgc aaagcacaaa
     5401 ctgatgaagt gctggaaaac ccggacccgc gaggtgcggc atgaaacagg tttgcgtcct
     5461 cggtaacggg cagttaggcc gtatgctgcg tcaggcaggc gaaccgttag gcattgctgt
     5521 ctggccggtc gggctggacg ctgaaccggc ggcggtgcct tttcaacaaa gcgtgattac
     5581 cgctgagatc gaacgctggc cggaaacagc attaacccgc gagctggcgc gccatccggc
     5641 ctttgtgaac cgcgatgtgt tcccgattat tgctgaccgt ctgactcaga agcagctttt
     5701 cgataagctc cacctgccga ctgcaccgtg gcagttactt gccgaacgca gcgagtggcc
     5761 tgcggtgttt gagcgtttag gtgagctggc gattgttaag cgtcgcactg gtggctatga
     5821 cggacgcggt caatggcgtt tacgcgccga tgaaaccgag cagttaccgg ctgaatgcta
     5881 cggcgaatgt attgtcgagc agggcataaa cttctctggt gaagtgtcgc tggttggcgc
     5941 gcgctgcttt gatggcagca ccgtgtttta tccgctgacg cataacctgc atcaggacgg
     6001 tattttgcgc accagcgtcg cttttccgca ggccaatgcg cagcagcaag cgcaagccga
     6061 agagatgctg tcggcgatta tgcaggagct gggctatgtg ggcgtgatgg cgatggagtg
     6121 ttttgtcacc ccgcaaggtc tgctgatcaa cgaactggca ccgcgtgtgc ataacagcgg
     6181 tcactggaca caaaacggtg ccagcatcag ccagtttgag ctacatctgc gggcgattac
     6241 cgatctgccg ttaccgcaac cagtggtgaa taatccgtcg gtgatgatca atctgattgg
     6301 tagcgatgtg aattatgact ggctgaaact gccgctggtg catctgcact ggtacgacaa
     6361 agaagtccgt ccgggccgta aagtggggca tctgaatttg accgacagcg acacatcgcg
     6421 tctgtccgcg acgctggaag ccttgatccc gctgctgccg ccggaatatg ccagcggcgt
     6481 gatgtgggcg cagagtaagt tctgttaacc tggtgctcat ttgattgccg gatgcggcgt
     6541 gaacgcctta tccggcctac accgatccgg tacccattgt aggcctgata agacgcgtca
     6601 agcgtcgcat caggcatggt gcgccgattg ccggatgctc tcatcactac agcgaaatac
     6661 aggtccccgc ttcgcccgcc agcgtctctt caattcgcga taacgcccca atccacgcgg
     6721 gtttaccacg gcttctgaca taaccactca ccgccgttac cttcggcccc atcgaaccat
     6781 cggctttggc aaatggcgct aactcatccg gtgtggcatg gcgaatggca cgttgctgcg
     6841 gcattcccca gttttcatat accgcatcag catcggtgag gatcaccagt ccatcggcgt
     6901 taatttgctc ggcgagcaac gcagcggcga gatctttatc aatcactgct tcactccctg
     6961 ctccgtcatc cgtcacaggc acaccgccgc cgccactgca aatcaccaca tgcccctctt
     7021 tgagcaacaa ctcgatggct tcgctgtcga gaattttacg cggttgcgga gacgccacca
     7081 cccggcgcaa atatttacca tcacgtttca tctgccagcc gtaagccgct tccagtgcct
     7141 cttgttcttc tggctgataa accggaccaa taaatttctc tggctgcaaa aacgccggat
     7201 catcaggcga aacttcaatg cgcgtccgca ccgtcgtgac aggcggcatc tgcggctgtg
     7261 cgctcaaact ctgcgccagc atatagccaa tcatcccctg actttctgcc accagcacat
     7321 ccagcggata cggttctacc tctttccacg ccagattctg caatgccagc agccccacct
     7381 gcggcccgtt gccgtgaaca atcgccagcc gataagaacg ggccaggcgc gccagcgcgg
     7441 gtacagcact ggcgatattg cgatattgat tttctgccgt cagcgcctca ccgcgctgga
     7501 gtaaggcgtt gcccccaaga gctacaacca gtgttttcat ggttttcctt gtaataattg
     7561 ttggccaagc cagaacccca gcagtgtatc tgcccctgac gtatgcccca gcgccagcag
     7621 cgaatcaatc gcaacagcgg tacgtttcgg gcaactcaga gcatgaacaa agtgcaggag
     7681 tggcgaggcg aaatatcctt gcgcggcata acgtaaataa ctgacgctca ccgctgtggt
     7741 aacgagttgc agattgtcgg aacaggcaaa aaacggacga ccggagcgcg catctaaagc
     7801 accataatac caggccgcca gcagcattcc gctcagcgtg tcatcatgac ttggcgttaa
     7861 tccggggcct ttacccagcc agtgccgcca gtcggtctta acgccattga gcgcggcctg
     7921 aaaacagtga cgaaactggc gtaactcagc aggcagcgga tcgcttgccg ccaacgccag
     7981 tggcccgaaa agcccggttt cctccgcgcg ttgcatccat gcagctgcaa gtggttgagg
     8041 atgcgcaggc ggcgtaatac gcagcaaaca ataacgctgt ggctgtttaa ccgtgaaacg
     8101 cccgaggcga atcccttgag ccacaacctg tggtcgttca ttgccgcata atccaccgca
     8161 taacgcatcg aattgcgcac ggcgaagcac ccatcctccg gggccgaaac cactaccctg
     8221 acgatgcagc gttaacagtt cgccgctttc cgtcatcagg ttaatcgccc gccgccacac
     8281 tcccgctaac cgccaggact ggcgataatt cggtgcgcta ctactggcaa gcagaggatg
     8341 gatgatcgtc attacgcgtt cctcaaccaa tccccatgct ttcagccagt gcttccagcg
     8401 cctgttcaaa gcacgccagc ggtgcccgca cggtgcctgc gccaatctgc ccgatccccg
     8461 cctctttatg ggcgataccg gtattgatga gtggcgtaat gccggtttct accacgcgac
     8521 gaatgtccag tcccaggcac gcgccctgaa aatcccagct tgggatctgc aactgcatat
     8581 tgcgttcaag gtaaatttcc gccatctctt cagataccgc cctggccgct tccatgccac
     8641 tcgcaccgac aaagcgcgtt acgccaggcg ctgcgatcat tgccgcacct ccgataccaa
     8701 aggtttcggt aatcgcgcta tcgcccatat ccgggttcgc ctgctcctgc gagaagccgg
     8761 tgaaaaacag accttgcgga gtgtttacag gcgcagtaaa ccagcgttcg cccagcccgc
     8821 ttacccgaat cccgaacata ttgccgttgc gggtcattgc cgtgacgatg ctgcctgcgc
     8881 ggatcatcgc gccagcatcc atcgccgcct tgcagtaagc catcgcgagg ttgaggaaga
     8941 actgatcggt cacgctgagg aaatccatca cttccgcgat gtgctgttta tcatgatcga
     9001 ggcgagcaat ttgtggggcc agcgcacgca ttaacagtgc tgaggaagca atattgcgtt
     9061 gatggaactc atcgcccatc gtaatgccct gcgccatcat cgcagtaaga tcgataccgc
     9121 gctccatgcg ccccagcgcc gcgcttaata ctggcattaa cacatcgcgc atccagcgat
     9181 ggcgagtcag gacatcttcg ccgtaagcgc caaaacgcat cactttgccg ataccttcgt
     9241 tgaggttgca gtacgcccgg ttgccgtcgg tcacgttctc gaccaccagc atcggcatac
     9301 tggcagaagt aataccgccc attggcccga cggcattcac atggtgacaa ggaatgaagt
     9361 tcacttcccc ctgctccagt attgccagcg cctgcgcttc atctttcgcc caaccttcga
     9421 acagacatgc gcccacgcac gcccctttca tgggtccggt catctcctgc cagcgcattg
     9481 gcggcccggt gtgaagcagt gttttgccct cgtttagttc gctgataagt gaagaagccg
     9541 gttgcacatc cagccagtgt ggacgagcgc gacgaatttg ttcgataacc gcagcattgg
     9601 cttgcgccac tgatgtaaac atgagaaccc cttattgcaa acgttctaat aaacgagcca
     9661 gttttttatt gccgccggcg actggcgacc attggtaatg cacaaccggt ttgctggcgc
     9721 tttgtagctc cagcgcaaaa ctgcgtaatc cgatgttaat cacggcgacg ttttccagta
     9781 atgacggtgt gtgttgctgt gcggcaggcg agagcggatg aattaacgct gccgccagca
     9841 aggtggcttc cggtagcgaa ctcacgaccg caatccccgc atcttccagc gtggcgattt
     9901 gctgcgagcg gcattgcggg tcacgttcag tgcctgtcac cgtggcaatg gcatacagtg
     9961 gttgattgtc tgaacgcgca gcacaggctt tttgccaggc gcttaccagc gaggcggcag
    10021 gatcggcggt cgcaccgaag ccaatcacga catcaagcag cagcacacgc acttgcggtt
    10081 tagcgccgag atcggtaatt aactggttgc gtaaggttgg gtcgatcatc ggatggggac
    10141 gcccgacggt gtagaaatca tcgccgaggt caataatctg gtggccatcg gcgtccagca
    10201 tcatgccgtg atgatgggca tcatcggctt ccacgccaag gtgtccggca agtaatcccg
    10261 ccgcttcggc agccagcgta ccgccggtat acaaaccgca aataaatccg ctgctggcag
    10321 gcattatcgc gttacgtcgc gccgtaacgc gtgaaagcag gcaagccagg cgtgcggcct
    10381 catccagcga ggaggcaaac cagacattct cgtcgcgggc caccgccggg gtataaccta
    10441 aaaacagcgc caccgtcggt ttgccggttg ctttcatggc attaacaatt ttcagacgca
    10501 ccgcttcggc aggtggtttt gaaacaaatg ccagcacttc gcttttctcg tctgcactga
    10561 gcatttccag cgccgtcagc gcactgatgc cgcccacttc acggctgagg tcgcggccgc
    10621 caaggccaat cgcgtgagta attccctccc ctgccagcgc aatctgcgag cacagctcct
    10681 gaatcccggt accggaagcg ccaatgacgc caatgttgcc ttccggcatc acgttagcaa
    10741 aagccagcgg cgtgccggca atcatcgacg taccgcagtc cggccccatc accagcaagc
    10801 ctttttcccg cgcgcgggtt ttaagttgga tttcatcttc cagcgtgacg ttatcggaga
    10861 acatcatcac gttgaggttg cgatccagcg cctgattcgc cagctccgcc gcatactcgc
    10921 cagccactga aatcagcgcc agattggcat cgggtaattt ctgacaggca ctgtcccagc
    10981 gacgcacctg cgtcaacgcc tggctgctgc ctgacccctg cgccagttgt tttagcgcct
    11041 cttcaagctg ctgcataatc gcctgcgcga tccccgcatc cgccgcttcg ctacgaattg
    11101 ccacgcaaat atcgttcggc gtggcgttat taaaatcgtc atgccagaaa cctgtggtat
    11161 ctaataacgc tttattggcg ggcgtaccca tcattacgga aacatcatca acattttctg
    11221 attcgctgag ttttcgtgaa ataatcatta aactgaccga atcctgaaaa cacccttttt
    11281 taataaaggc gtggatcata acaactcctt aaaaattgca cccatggcaa ttgcacgagc
    11341 agtgttaatg tggcgattac ggtatcgatg acaacgagta aataaggtga tgaatatcac
    11401 acagggatta ttaacaaaag aa
//
LOCUS       DBFIFM010000143        11374 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.143, whole genome shotgun sequence.
ACCESSION   DBFIFM010000143 DBFIFM010000000
VERSION     DBFIFM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.143"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            120..1595
                     /gene="zwf"
                     /locus_tag="N7C74_003384"
     CDS             120..1595
                     /gene="zwf"
                     /locus_tag="N7C74_003384"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HGU2433193.1"
                     /translation="MAVTQTAQVCDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            1830..3641
                     /gene="edd"
                     /locus_tag="N7C74_003385"
     CDS             1830..3641
                     /gene="edd"
                     /locus_tag="N7C74_003385"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HGU2433194.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            3678..4319
                     /gene="kdgA"
                     /locus_tag="N7C74_003386"
                     /gene_synonym="eda"
     CDS             3678..4319
                     /gene="kdgA"
                     /locus_tag="N7C74_003386"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HGU2433195.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(4375..5553)
                     /gene="purT"
                     /locus_tag="N7C74_003387"
     CDS             complement(4375..5553)
                     /gene="purT"
                     /locus_tag="N7C74_003387"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HGU2433196.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYCESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            5687..5977
                     /gene="yebG"
                     /locus_tag="N7C74_003388"
     CDS             5687..5977
                     /gene="yebG"
                     /locus_tag="N7C74_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HGU2433197.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            6044..6400
                     /gene="yebF"
                     /locus_tag="N7C74_003389"
     CDS             6044..6400
                     /gene="yebF"
                     /locus_tag="N7C74_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HGU2433198.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            6727..7386
                     /locus_tag="N7C74_003390"
     CDS             6727..7386
                     /locus_tag="N7C74_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HGU2433199.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLIGGGAVAGTVLWNKYKDKIRAAHQDEPQFGSQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            7595..9655
                     /gene="ptrB"
                     /locus_tag="N7C74_003391"
     CDS             7595..9655
                     /gene="ptrB"
                     /locus_tag="N7C74_003391"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HGU2433200.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRNHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDLQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     RPAD"
     gene            complement(9652..10314)
                     /gene="exoX"
                     /locus_tag="N7C74_003392"
     CDS             complement(9652..10314)
                     /gene="exoX"
                     /locus_tag="N7C74_003392"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HGU2433201.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVHPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADMTGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(10338..10994)
                     /gene="yobB"
                     /locus_tag="N7C74_003393"
     CDS             complement(10338..10994)
                     /gene="yobB"
                     /locus_tag="N7C74_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HGU2433202.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(11096..11326)
                     /gene="holE"
                     /locus_tag="N7C74_003394"
     CDS             complement(11096..11326)
                     /gene="holE"
                     /locus_tag="N7C74_003394"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HGU2433203.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
BASE COUNT         2849 a         2820 c         2989 g         2716 t
ORIGIN      
        1 cacgggtgga taagcgttta cagttttcgc aagctcgtaa aagcagtaca gtgcaccgta
       61 agaaaattac aagtataccc tggcttaagt accgggttag ttaacttaag gagaatgaca
      121 tggcggtaac gcaaacagcc caggtctgtg acttggtcat tttcggcgcg aaaggcgacc
      181 ttgcgcgtcg taaattgctg ccttccctgt atcaactgga aaaagccggt cagctcaacc
      241 cggacacccg gattatcggc gtagggcgtg ctgactggga taaagcggca tataccaaag
      301 ttgtccgcga ggcgctcgaa actttcatga aagaaaccat tgatgaaggt ttatgggaca
      361 ccctgagcgc acgtctggat ttttgtaatc tcgatgtcaa tgacactgct gcattcagcc
      421 gtctcggcgc gatgctggat caaaaaaatc gtatcaccat taactacttt gccatgccgc
      481 ccagcacttt tggcgcaatt tgcaaagggc ttggcgaggc aaaactgaat gctaaaccgg
      541 cacgcgtagt catggagaaa ccgctgggga cgtcgctggc gacctcgcag gaaatcaatg
      601 atcaggttgg cgaatacttc gaggagtgcc aggtttaccg tatcgaccac tatcttggta
      661 aagaaacggt gctgaacctg ttggcgctgc gttttgctaa ctccctgttt gtgaataact
      721 gggacaatcg caccattgat catgttgaga ttaccgtggc agaagaagtg gggatcgaag
      781 ggcgctgggg ctattttgat aaagccggtc agatgcgcga catgatccag aaccacctgc
      841 tgcaaattct ttgcatgatt gcgatgtctc cgccgtctga cctgagcgca gacagcatcc
      901 gcgatgaaaa agtgaaagta ctgaagtctc tgcgccgcat cgaccgctcc aacgtacgcg
      961 aaaaaaccgt acgcgggcaa tatactgcgg gcttcgccca gggcaaaaaa gtgccgggat
     1021 atctggaaga agagggcgcg aacaagagca gcaatacaga aaccttcgtg gcgatccgcg
     1081 tcgacattga taactggcgc tgggccggtg tgccattcta cctgcgtact ggtaaacgtc
     1141 tgccgaccaa atgttctgaa gtcgtggtct atttcaaaac acctgaactg aatctgttta
     1201 aagagtcgtg gcaggatctg ccgcagaata aactgactat ccgtctgcaa cctgatgaag
     1261 gcgtggatat ccaggtactg aataaagttc ctggccttga ccacaaacat aacctgcaaa
     1321 tcaccaagct ggatctgagc tattcagaaa cctttaatca gacgcatctg gcggatgcct
     1381 atgaacgttt gctgctggaa accatgcgtg gtattcaggc actgtttgta cgtcgcgatg
     1441 aagtggaaga agcctggaaa tgggtagact ccattactga ggcgtgggcg atggacaatg
     1501 atgcgccgaa accgtatcag gccggaacct ggggacccgt tgcctcggtg gcgatgatta
     1561 cccgtgatgg tcgttcctgg aatgagtttg agtaatatct gcgcttatcc tttatggtta
     1621 ttttaccggt aacatgatct tgcacagatt gtagaacaat ttttacactt tcaggcctcg
     1681 tgcggattca cccacgaggc tttttttatt acactgactg aaacgttttt gccctatgag
     1741 ctccggttac aggcgtttca ctcataaatc ctctgaatga aacgcgttgt gaatcatcct
     1801 gctctgacaa ctcaatttca ggagccttta tgaatccaca attgttacgc gtaacaaatc
     1861 gaatcattga acgttcgcgc gagactcgct ctgcttatct cgcccggata gaacaagcga
     1921 aaacgtcgac cgttcatcgt tcgcagttgg catgcggtaa cctggcacac ggtttcgctg
     1981 cctgccagcc agaagacaaa gcctctttga aaagcatgtt gcgtaacaat atcgccatca
     2041 tcacctccta taacgacatg ctctccgctc accagcctta tgaacactat ccagaaatca
     2101 ttcgtaaagc cctgcatgaa gcgaatgcgg ttggtcaggt tgcgggcggt gttccggcga
     2161 tgtgtgatgg tgtcacccag gggcaggatg gaatggaatt gtcgctgcta agccgcgaag
     2221 tgatagcgat gtctgcggct gtcgggctgt cccataacat gtttgatggt gctctgttcc
     2281 tcggggtgtg cgacaagatt gtcccgggtc tgacgatggc agccctgtcg tttggtcatt
     2341 tgcctgcggt gtttgtgccg tctggaccga tggcaagcgg tttgccaaat aaagaaaaag
     2401 tgcgtattcg ccagctttat gccgaaggta aagtggaccg catggcctta ctggagtcag
     2461 aagccgcgtc ttaccatgcg ccgggaacat gtactttcta cggtactgcc aacaccaacc
     2521 agatggtggt ggagtttatg gggatgcagt tgccaggctc ttcttttgtt catccggatt
     2581 ctccgctgcg cgatgctttg accgctgccg ctgcgcgtca ggttacacgc atgaccggta
     2641 atggtaatga atggatgccg atcggtaaga tgatcgatga gaaagtggtg gtgaacggta
     2701 tcgttgcact gctggcgacc ggtggttcca ctaaccacac catgcacctg gtggcgatgg
     2761 cgcgcgcggc cggtattcag attaactggg atgacttctc tgacctttct gatgttgtac
     2821 cgctgatggc acgtctctac ccgaacggtc cggccgatat taaccacttc caggcggcag
     2881 gtggcgtacc ggttctggtg cgtgaactgc tcaaagcagg cctgctgcat gaagatgtca
     2941 atacggtggc aggctttggt ctgtctcgtt atacccttga accatggctg aataatggtg
     3001 aactggactg gcgggaaggg gcggaaaaat cactcgacag caatgtgatc gcttccttcg
     3061 aacaaccttt ctctcatcat ggtgggacaa aagtgttaag cggtaacctg ggccgtgcgg
     3121 ttatgaaaac ctctgccgtg ccggttgaga accaggtgat tgaagcgcca gcggttgttt
     3181 ttgaaagcca gcatgacgtt atgccggcct ttgaagcggg tttgctggac cgcgattgtg
     3241 tcgttgttgt ccgtcatcag gggccaaaag cgaacggaat gccagaatta cataaactca
     3301 tgccgccact tggtgtatta ttggaccggt gtttcaaaat tgcgttagtt accgatggac
     3361 gactctccgg cgcttcaggt aaagtgccgt cagctatcca cgtaacacca gaagcttacg
     3421 atggcgggct gctggcaaaa gtgcgcgacg gggacatcat tcgtgtgaat ggacagacag
     3481 gcgaactgac gctgctggta gacgaagcgg aattggctgc tcgcgaacca cacattcctg
     3541 acctgagcgc gtcacgcgtg ggaacaggac gtgaattatt cagcgccttg cgtgaaaaac
     3601 tgtccggtgc cgaacagggc gcaacctgta tcacttttta agacgacaaa tttgtaatca
     3661 ggcgagagaa aactctgatg aaaaactgga aaacaagtgc agaatcaatc ctgaccaccg
     3721 gcccggttgt accggttatc gtggtaaaaa aactggaaca cgcggtgccg atggcaaaag
     3781 cgttggttgc tggtggggtg cgcgttctgg aagtgactct gcgtaccgag tgtgcagttg
     3841 acgctatccg tgctatcgcc aaagaagtgc ctgaagcgat tgtgggtgcc ggtacggtgc
     3901 tgaatccaca gcagctggca gaagtcactg aagcgggtgc acagttcgca attagcccgg
     3961 gtctgaccga gccgctgctg aaagctgcta ccgaagggac tattccgctg attccgggga
     4021 tcagcactgt ttccgaactg atgctgggta tggactacgg tttgaaagag ttcaaattct
     4081 tcccggctga agctaacggc ggcgtgaaag ccctgcaggc gatcgcgggt ccgttctccc
     4141 aggtccgttt ctgcccgacg ggtggtattt ctccggctaa ctaccgtgac tacctggcgc
     4201 tgaaaagcgt gctgtgcatc ggtggttcct ggctggttcc ggcagatgcg ctggaagcgg
     4261 gcgattacga ccgcattact aagctggcgc gtgaagctgt agaaggcgct aagctgtaaa
     4321 agtcaaaatg cccgatcgag gatcgggcat ttttgtagcg tttatttgcc gggtttaacc
     4381 ctgtactttt acctgtccgg cggcgtgctt cgcgcgttca atggcgtcaa caacactctc
     4441 tgcagtagcc agtgccaccc ccagacgacg gctgccatca atttccggct taccaaataa
     4501 acgaatctgc aaatctgcgc ctacggcatt ctgcacatta tcaaacgtga cattctgact
     4561 ggtcagttgt ggcagaataa cggcagaagc tgcaggacca tactgacgga tcccgccaac
     4621 cggaagtccg aggaaggcac gtacatgcag ggcaaactct gagagatctt gagaaattaa
     4681 cgtcaccatc ccggtatcat gtggacgagg ggagacctca ctgaaaatca cctcatcacc
     4741 acagacaaac agctcgacac caaacagacc ataaccgccc agtgccagca ccactttacg
     4801 ggcaatctcc tgcgcacgtt caagggcaag cgggctcatt tgctgtggtt gccaggattc
     4861 acagtagtcg ccatcttcct ggcgatgacc tactggtgca cagaaatgga cgccatccac
     4921 cgcgctgacg gttagcaggg taatttcgaa gtcaaactta acgacgcctt caacaattac
     4981 gcggcccgct ccggcgcgac cgccttgctg agcgtattcc catgcctgag caagttgctc
     5041 tgcagagcga ataaacgtct gccccttgcc ggaagagctc atcaccggtt ttacaatgca
     5101 gggatagcca atggcggcaa ccgcctcgcg gaaaaggctt tcgctatcgg caaaacgata
     5161 agtggaagtg ggcagctgca gctcttctgc cgccaggcga cggataccct cgcgattcat
     5221 cgttaatttc gttgcgcgag cgcaggggac aacattcagt ccttcctctt caagttggat
     5281 cagcatatcg gtggcaatag cttcgatctc cggcacgata taatgtggtt tttccagttc
     5341 aaccacacgg cgtaatgcat caccatcaag catattaatg acatgggagc gatgcgcgac
     5401 atgcatggct ggtgcgtcgg catagcgatc gacggcaatc acctctacgc cgagacgctg
     5461 acactcgatt gccacttctt tacccagttc accggagcct aataacatca cgcgagttgc
     5521 tgccggacgc agcgcagtgc ctaataacgt cataacgaat atcccctgat taattccgcg
     5581 cgcagtataa acgaaaacgt ttgcgtgtgt ctttattgct ggtgttgatc tctcaaccga
     5641 aaagaaatat actgtataga atcacagtta ttatgagagg ctgcaaatgg cggttgaagt
     5701 taaatacgta gtcattcgtg agggcgaaga gaaaatgtcg tttaccagca aaaaggaagc
     5761 cgatgcttat gacaaaatgc ttgatacggc ggatctgctt gacacctggc tgacaaattc
     5821 tccagtgcaa atggaagacg agcaacgtga agccctttcg ctatggctgg cagaacaaaa
     5881 agatgtgctg agcaccattc tgaaaaccgg caaattaccg tccccacagg tcgtcggtgc
     5941 tgaatccgaa gaggaagatg cctcacacgc ggcctgaagc aacttgcgct cctgacaatt
     6001 tgtaccatgc tttctcctga atcattgtgc tatggagaaa aacatgaaaa aaagaggggc
     6061 gtttttaggg ctgttgttgg tttctgcctg cgcatcagtt ttcgctgcca ataatgaaac
     6121 cagcaagtcg gtcactttcc caaagtgtga aggtctggat gctgccggaa ttgccgcgag
     6181 cgtaaaacgt gattatcaac aaaatcgcgt ggcgcgctgg gcagatgatc aaaaaattgt
     6241 cggtcaggcc gatcccgtgg catgggtcag tttgcaggac attcagggta aagatgataa
     6301 atggtcagta ccgctaaccg tacgtggtaa aagtgccgat attcattacc aggtcagcgt
     6361 ggactgcaaa gcgggaatgg cggaatatca gcggcgttaa tgggttctga taattcgatt
     6421 gaccgttggc ggatgcggcg cgagtgcctt gtccgcccca gaacgcacca caatcgtagg
     6481 cctgagaaga cgcaacagcg tcgcatcggg caatcagcat caaacaatgc cactcaaaac
     6541 gacaaactac tttcacctaa aataccctta tcacctgata tacagtaaat accttacctg
     6601 gcgcgaattt ctttgaataa cggcctaccg ccccgcttat ttgcgcatct tttgtctcga
     6661 tatacatttt ctgacatcct catcctctat gctacaggca ctaaaaatgg ttattgagga
     6721 gcaaatatgg ctaactggtt aaatcaactg caatccctgc ttgggcaaag cagttcttct
     6781 acctcctcgt ctgcggatca gggattgggc aaacttttag tgccgggcgc attaggcgga
     6841 ctggctggac tgctggtcgc aaataaatca gcacgtaaac ttcttacgaa atatggcacc
     6901 aacgcgttgc tgattggcgg cggagcggta gcgggtacgg tgctgtggaa taaatacaaa
     6961 gataaaattc gcgcggcgca tcaggacgaa ccgcagtttg gctcgcaaag tacgccgctg
     7021 gatgagcgta cggaacgttt gatccttgcg ctagtctttg ccgctaaaag tgatggtcat
     7081 attgatgcca aagaacgtgc ggcaatcgac cagcaattgc gtgaagccgg cgtggaagag
     7141 caggggcggg tactcattga gcaggcaatc gaacaaccgc tggatccaca acgcctggct
     7201 accggggtcc gcaatgaaga agaggcgctg gaaatctatt tcctgagctg cgcggctatt
     7261 gatattgacc attttatgga acgcagttac ctgaatgcac tgggtgacgc gctgaaaatc
     7321 ccccaggatg ttcgtgaagg cattgaacgc gatctcgaac agcaaaaacg cactttggcg
     7381 gaataattcc gctctctgga tagcctgaag ttgactattt cgggctatcc agaacagcac
     7441 agccatttga acaggctgat tttctctgtc tgcataacat tcgatattcg cgccattacc
     7501 tgtcatcatc taagcaatga ctgccctgtt tcgcttgcat ccccggtgag ttttgccacc
     7561 cttataagat gtttcaacca gaaagaacaa taacatgcta ccaaaagccg cccgcattcc
     7621 ccacgccatg acgcttcatg gcgatacgcg catcgataat tactactggc tgcgggacga
     7681 tacgcgttct cagccggaag tcctggacta cctgcaacaa gaaaatagtt acggtcatcg
     7741 ggtgatggcc tcacaacaag ccttgcagga tcgcatctta aaggaaatca tcgaccgcat
     7801 tccgcaacga gaagtttctg cgccctacat caaaaatggc taccgctacc ggcatattta
     7861 tgaaccaggc tgtgaatatg ctatctacca gcgtcaatcg gcgttcagtg aagagtggga
     7921 cgagtgggaa atattgctcg atgccaacaa gcgcgcggct catagtgagt tttattcgat
     7981 gggcggaatg gcgattacgc ccgataacac cattatggcg ctggcagaag attttctttc
     8041 ccgacgccag tacggcattc gttttcgtaa tctggaaaca ggtaactggt acccggaact
     8101 gctggataac gttgaaccca gctttgtctg ggcaaatgac tcctggactt tctactatgt
     8161 tcgcaagcat ccggtgacgc tgctgcctta tcaggtctgg cgtcacgcca tcggtacgcc
     8221 agcatcgcaa gataaactga tttacgaaga aaaagacgat acctattacg tcagcctgca
     8281 taaaacgacc tcaaagcact atgtagtcat tcatttggcc agcgccacca ccagtgaagt
     8341 tcgcctgctg gacgcggaaa tggcagatgc cgagccgttt gtttttctgc cgcgccgcaa
     8401 agatcacgaa tacagccttg atcactacca gcatcgtttt tatctgcgtt ccaaccgcca
     8461 cggcaaaaac tttggcttat accgtacccg tatgcgtgat gagcaacagt gggaagagtt
     8521 aattccgcca cgcgaaaaca tcatgctgga agggtttacg ctgtttaccg actggctggt
     8581 ggttgaagag cgtcagcgcg ggttaaccag tttgcgccaa attaaccgca agacccggga
     8641 agtcattggt attgcctttg atgatccagc ctatgtgacc tggattgcct acaatccaga
     8701 acctgaaacc gcgcgattgc gttatggtta ttcttccatg accacaccag acactttgtt
     8761 tgaactggat atggataccg gtgagcgtcg tgtattaaaa caaacagaag ttcctggttt
     8821 tgatgcggcg aattaccgca gtgaacacct gtggatagtc gcccgtgatg gtgtcgaagt
     8881 tccggtttcg ttggtctacc atcgcaatca ttttcgcaaa ggacacaacc cgctgctggt
     8941 gtatggctac ggttcttacg gcgcaagtat tgatgccgat ttcagtttta gccgcttgag
     9001 tttgttagat cgtggctttg tctacgccat tgtccatgtt cgcggcggcg gtgagctggg
     9061 gcaacaatgg tacgaagacg gtaaatttct gaagaagaaa aatacgttta atgattatct
     9121 tgatgcctgc gatgcattgt taaaactggg ctatggctct ccttcgcttt gttatgcgat
     9181 gggcgggagt gcggggggca tgttgatggg cgttgcaatt aatcaacgcc cggaattatt
     9241 ccacggcgtt atcgcccagg taccgtttgt tgatgttgta acaacgatgc ttgatgaatc
     9301 aattcctctt accactggtg agtttgaaga gtgggggaat ccgcaggatc tgcaatatta
     9361 cgagtatatg aaaagctaca gcccgtatga caacgtcacc gcacaggctt atccacattt
     9421 actggtaacg accggtttac acgattctca ggtgcaatat tgggaaccgg caaaatgggt
     9481 cgctaagttg cgcgagctga aaaccgataa ccatctttta ttgctctgta ccgacatgga
     9541 ctcaggccat ggcggtaaat ctggtcgctt taaatcgtac gaaggcgtag cgatggaata
     9601 tgcttttctg gtcgcgctgg cgcagggaac attacccgct aggcctgcgg attaggtatt
     9661 ttccagataa tgtttcagtg ttaaacgcag ctccgggctc atgctgtcca ggttattaaa
     9721 caaccagcgc agatagcccg gatcgcgttc ggcaacgtcg gaaaccgctt tgccacggta
     9781 tttgccaaag gtgaaggtcg tcatcaacga cggacgtccg gtcatatcgg ccatctgttc
     9841 tgccgtccag ccggaggtgt tcataatatc gataagcaac gcggcggtga tataacagtc
     9901 atacaacgcg cggtgatgat gcaggcccgg cggcgtctgt acattgagct tccgtgtttt
     9961 atataacgcc atattgctgt acttgatccc tggccacaaa cgacgggcca gtttcatagt
    10021 gcaaatccac tcaccgggca tctcaggcag tacgcggcgg tcaaagctgg cgttatgcgc
    10081 gacataccat tcactaccgt aatagtgtgg gatcacatct tcaatccacg gtttatcggc
    10141 gaccatggct tcggtgatgc gatgaatcgc catcgcttgt ggactaatag gacgatcggg
    10201 gtgcaccagg tggctcatgg ggttgacgat tttcccgtca atgacatcaa cagaggcaat
    10261 ctcaacgatc cctccctgca aaccgcaggt ttctgtatcg ataatgcgca acatggcatg
    10321 ctccaggccg aaaaagccta gcgtaatgga atgatatcgc cttgccaacc ctgtgatgaa
    10381 cgctgaccga ctaacaataa tgaaccacga tcggcgcgaa cgatgagacg accatgttca
    10441 tcccatagtg cactgttacc acgggcattt gccatcagca cagcaatcga gtactgatgt
    10501 gaaaaaaatt gtaagcgacg ggccgatgcc agcaggtcgg gttctcccag acattgccct
    10561 gtcgtaaaca gtgaacaggt cgggtccata tccatacctt gcggttgttc atcgaccacc
    10621 gtgatggttc tggaacgacg gcccagacat gcaccatgac tctgatggta gatcccaggc
    10681 gtttttcgcc acggtgcgaa caccgcaatt ccacgaataa agcgatcgtt atattcaacg
    10741 ggaaggccgg caatgatggt cattcgccag gtcgttgcgg cataacaaag cgggtctaac
    10801 agtgagagat cgggtggggc aggaagggca cgtcgcgaat aatcacaccc cagtaaagaa
    10861 agcgatggaa aaactaacag ctgacattgt tgcctggcgg cggccctcac aaactccaga
    10921 tgatgagcta cctgttcggt gagtgaggtt ttacgaggct catattgcgc tgctgcaact
    10981 ttccagaacg acataatgac ttccttttca aaatgactgc aaattatggg tgcggtttag
    11041 aaaatactca taatttagca ggcgttatgt aagaaagtgt aactctgtat aagttttatt
    11101 taagtttggg ctcgtaaggt aaacgtgaca gattgaccga agccaaacgg tgggcaataa
    11161 gccgctcgcg aaaccagctg cgcaaatgtt caggctgttc acgttcaacc gcttcagcga
    11221 tcactggcat attgtagcgt tctttaaatg ccaccccggc cgccgccaaa tcgacattca
    11281 ctttatccat ttctgtttga tccagtttag ccagattctt cagcattctt aatctcctca
    11341 tcattcgcgg gcaaaagtta ctaagagtga gcct
//
LOCUS       DBFIFM010000144        11302 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.144, whole genome shotgun sequence.
ACCESSION   DBFIFM010000144 DBFIFM010000000
VERSION     DBFIFM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.144"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(146..322)
                     /locus_tag="N7C74_003395"
     CDS             complement(146..322)
                     /locus_tag="N7C74_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433204.1"
                     /translation="MEKDLKELREYLLLSPVYSEINDCLASLSMEDVTNEEFAERLGI
                     APDWMDAIDTSQLA"
     gene            625..1251
                     /locus_tag="N7C74_003396"
     CDS             625..1251
                     /locus_tag="N7C74_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU2433205.1"
                     /translation="MNVRIYCRASTEGQHADRALTSLREFSKSKGWQIAGEYIENASG
                     AKLERVELMRLLSEAQSGDLLLVEAIDRLSRLEHSAWVELKDTLNRKGLIIVSMDLPT
                     SWQMVEMAGNDLTSGILRAVNAMLIDILATMARQDYETRRKRQQQGIERAKSEGIYIG
                     RAKNQEAREIVREMLEQGVKPELIMKAAGISRATYYRIKNELLIVKSE"
     gene            1602..1769
                     /locus_tag="N7C74_003397"
     CDS             1602..1769
                     /locus_tag="N7C74_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGU2433206.1"
                     /translation="MLHSAMHSLMNSQARDTRDRSQNDDNVLEISEPCIRSTFERLYQ
                     NMNEVFGLGDY"
     gene            complement(1849..3096)
                     /locus_tag="N7C74_003398"
     CDS             complement(1849..3096)
                     /locus_tag="N7C74_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HGU2433207.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(3168..4082)
                     /locus_tag="N7C74_003399"
     CDS             complement(3168..4082)
                     /locus_tag="N7C74_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HGU2433208.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTEIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVDCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            4298..5731
                     /gene="cscA"
                     /locus_tag="N7C74_003400"
     CDS             4298..5731
                     /gene="cscA"
                     /locus_tag="N7C74_003400"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HGU2433209.1"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFVAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVVQHGALWQLG"
     gene            complement(5739..6734)
                     /locus_tag="N7C74_003401"
     CDS             complement(5739..6734)
                     /locus_tag="N7C74_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU2433210.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGSVTRIPCPLLIRCST"
     gene            6977..7195
                     /locus_tag="N7C74_003402"
                     /pseudo
     CDS             6977..7195
                     /locus_tag="N7C74_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            7213..8541
                     /gene="dsdA"
                     /locus_tag="N7C74_003403"
     CDS             7213..8541
                     /gene="dsdA"
                     /locus_tag="N7C74_003403"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HGU2433211.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMEQYLAKGR"
     gene            complement(8649..10187)
                     /gene="emrY"
                     /locus_tag="N7C74_003404"
     CDS             complement(8649..10187)
                     /gene="emrY"
                     /locus_tag="N7C74_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HGU2433212.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     SINLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVIFLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTETIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(10187..>11302)
                     /gene="emrK"
                     /locus_tag="N7C74_003405"
     CDS             complement(10187..>11302)
                     /gene="emrK"
                     /locus_tag="N7C74_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HGU2433213.1"
                     /translation="FSLLAIVLFIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQ
                     VSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQY
                     SAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQAYKA
                     NKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQ
                     SLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNA
                     FSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKNEDIAEMPE
                     LASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         3242 a         2705 c         2559 g         2796 t
ORIGIN      
        1 caattaggtt taaaaattgt gatacttatg acagatttga gtgacatttt atcaatctac
       61 tgattttgat ggtgaaattt attagaaggt atgcgtagtg ctacgggaga aatgagaaac
      121 gcgcccagca atggcgcatc ctttcttaag caagttggct agtatctata gcatccatcc
      181 aatcaggagc aattcccaaa cgttccgcga actcttcatt ggtcacgtct tccattgaga
      241 gactagccag acaatcgtta atttcagaat agacggggga aagtaatagg tattcccgca
      301 actctttcag gtctttttcc atcttaacac tccttgttaa tagaccgtcg aacaatggta
      361 cagatttgta ccattccaag tactggcaag caaccccacc agcacaacaa attatctcat
      421 acggtaaaaa cggtgtcggg ctgtttcttt cggctagctc aatatatcac cttaaacacc
      481 agccgttatc attgtctcaa ttgggtatac cgtaatgaca aggtgataca aagtctcaat
      541 agggttgtat aggaacattt tgactacaca aactgcatta tgtatcattt gcacttatat
      601 tcacaaccct tttgagacaa tgcaatgaac gttagaattt actgccgagc atcaactgag
      661 gggcaacacg ctgatcgagc acttaccagc ctacgtgaat tttcaaaatc aaaaggctgg
      721 cagattgcag gagagtatat agaaaatgct agcggggcga agcttgagcg cgtagagctt
      781 atgcggctat tgtctgaagc ccagtccggc gatctgttgc tagtggaagc tatagatcgt
      841 ctgagtcgcc tagaacatag tgcatgggtc gaactgaaag atacgctcaa ccgaaaaggg
      901 ttaatcatcg tatcaatgga tttaccaacg agctggcaga tggtcgagat ggcaggcaat
      961 gatctaacca gcggcatcct gagagccgta aatgccatgt tgattgatat ccttgccaca
     1021 atggcacggc aggattacga aacacgccgt aaacgccaac agcaaggcat tgaacgagct
     1081 aaaagcgaag gcatatacat tgggagggcg aaaaatcagg aagcccgaga gatcgttaga
     1141 gagatgttgg agcaaggagt aaaacctgaa ctcatcatga aggcagcagg aatcagccga
     1201 gcgacttact accggataaa aaacgagcta ttgatcgtaa aatcagaata aaatttgaaa
     1261 ggtcgtcagg cttgtttcat caggtaaaaa ccagcatttc tgatcacaac gcttgggggc
     1321 acctattggg gcactcacca aaatcgaaag agaaataaac tacaattcaa attgttagct
     1381 aatttttcga ttcctgcagg gacaccagat acccttcaaa ccaaatctac cttcatcccg
     1441 taaacgatgg gtttggcagc acacttgccc tatatctact catttttact gcaacaggtt
     1501 gaaatctcag cactgtcaga aagcgctgat gactaaacag cccagggccg ggcgatgtaa
     1561 ccatcacaca gaatcctgat agcgaaatac ggcgtgactc gatacttcac tccgcaatgc
     1621 attccttgat gaattcgcag gcccgtgata cacgggacag gtcgcagaat gacgacaatg
     1681 tcctggaaat cagcgaaccg tgtatccgga gtacatttga gcgactgtac cagaacatga
     1741 atgaggtgtt tggattaggc gattattagc agggctaagc attttagtat tattattttc
     1801 cggttgaagg atatggagat atcgacaaca accggaaaaa gtttacgtct atattgctga
     1861 aggtacaggc gtttccataa ctatttgctc gcgtttttta ctcaggaaga aaatgccaaa
     1921 tagcaacatc aggcagacaa tacccgaaat tgcgaagaaa actgtctggt agcctgcgtg
     1981 gtcaaagagt atcccagtcg gcgttgaaag cagcacaatc ccaagcgaac tggcaatttg
     2041 aaaaccaatc agaaagatcg tcgacgacag gcgcttatca aagtttgcca cgctgtattt
     2101 gaagacggat atgacacaaa gtggaacctc aatggcatgt aacagcttca ctaatgaaat
     2161 aatccagggg ttaacgaaca gcgcgcagga aaggatacgc aacgccataa tcacaacacc
     2221 gataagtaat gcattttttg gccctacccg attcacaaag aacggaataa tcgccatgca
     2281 cagcgcttcg agtaccacct ggaatgagtt gagataacca tacaggcgcg ttcctacatc
     2341 gtgtgattcg aataaacctg cataaaagac aggaaaaagt tgttgatcaa aaatgttata
     2401 gaaagaccac gtcccaacaa taaatatgac gaaaacccag aagtttcgat ccttgaaaac
     2461 tgcgataaaa tcctcttttt ttacccctcc cgcatccgcc gctacgcact ggtgatcctt
     2521 atctttaaaa cacatgttga tcatcataaa tacagcgcca aatagcgaga ccaaccagaa
     2581 gttgatatgg ggactgatac taaaaaatat gccggcaaag aacgcgccaa tagcatagcc
     2641 aaaagatccc caggcgcgcg ctgttccata ttcgaaatga aaatttcgcg ccattttttc
     2701 ggtgaagctg tcaagcaaac cgcatcccgc cagatacccc aggccaaaaa agagcgcccc
     2761 cagaattaga cctacagaaa aattgctttg cagtaacggt tcataaacgt aaatcataaa
     2821 cggtccggtc aagaccagga tgaaactcat acaccagatg agcggtttct tcagaccgag
     2881 tttatcctga acgatgccgt agaacatcat aaatagaatg ctggtaaact ggttgaccga
     2941 ataaagtgta cctaattccg tccctgtcaa ccctagatgt cctttcagcc aaatagcgta
     3001 taacgaccac cacagcgacc aggaaataaa aaagagaaat gagtaactgg atgcaaaacg
     3061 atagtacgca tttctgaatg gaatattcag tgccataatt acctgcctgt cgttaaaaaa
     3121 ttcacgtcct atttagagat aagagcgact tcgccgttta cttctcacta ttccagttct
     3181 tgtcgacatg gcagcgctgt cattgcccct ttcgccgtta ctgcaagcgc tccgcaacgt
     3241 tgagcgagat cgataattcg tcgcatttct ctctcatctg tagataatcc cgtagaggac
     3301 agacctgtga gtaacccggc aacgaacgca tctcccgccc ccgtgctatc gacacaatcc
     3361 acagacattc cagcaaaatg gtgaacttgt cctcgataac agaccaccac cccttctgca
     3421 cctttagtca ccaacagcat ggcgatctca tactcttttg ccagggcgca tatatcccga
     3481 tcgttctgtg tttttccact gataagtcgc cattcttctt ccgagagctt gacgacatcc
     3541 gccagttgta gcgcctgccg caaacacaag cggagcaaat gctcgtcttg ccatagatct
     3601 tcacgaatat tgggatcgaa gctgacaaaa cctccggcat gccggatctc cgtcatcgca
     3661 gtaaatgcgc tggtacgcga aggctcggca gacaacgcaa ttgaacagag atgtaaccat
     3721 tcgccatgtc gccagcaggg caagtctgtc gtctctaaaa aaagatcggc actggggcgg
     3781 accataaacg taaatgaacg ttccccttga tcgttcagat cgacaagcac cgtggatgtc
     3841 cggtgccatt catcttgctt cagatacgtg atatcgactc cctcagttag cagcgttctt
     3901 tgcattaatg caccaaaagg atcatccccc acccgaccta taaacccact tgttccgcct
     3961 aatctggcga ttcccaccgc aacgttagct ggcgcgccgc caggacaagg cagtagccgc
     4021 ccgtctgatt ctggcaagag atctacgacc gcatccccta aaacccatac tttggctgac
     4081 atttttttcc cttaaattca tctgacttac gcatagtgat aaacctcttt ttcgcaaaat
     4141 cgtcatggat ttactaaaac atgcatattc gatcacaaaa tgtcatagtt aacgttaaca
     4201 tttgtgatat tcatcgcatt tatgaaagta agggacttta tttttataaa agttaacgtt
     4261 aacaattcac caaatttgct taaccaggat gataaaaatg acgcaatctc gattgcatgc
     4321 ggcgcaaaac gcactagcaa aacttcacga gcgccgaggt aacactttct atccccattt
     4381 tcacctcgcg cctcctgccg ggtggatgaa cgatccaaac ggcctgatct ggtttaacga
     4441 tcgttatcac gcgttttatc aacatcaccc aatgagtgaa cactgggggc caatgcactg
     4501 gggacatgcc accagcgacg atatgatcca ctggcagcat gagcctattg cgctagcgcc
     4561 aggagacgag aatgacaaag atgggtgttt ttcaggtagt gctgtcgatg acaatggtgt
     4621 cctctcactt atctacaccg gacacgtctg gctcgatggt gcaggtaatg acgatgcaat
     4681 tcgcgaagta caatgtctgg ctaccagtcg ggatggtatt catttcgaga aacagggtgt
     4741 gatcctcact ccaccagaag gaatcatgca cttccgcgat cctaaagtgt ggcgtgaagc
     4801 cgacacatgg tggatggtag tcggggcgaa agacccaggc aacactgggc agatcctgct
     4861 ttatcgcggc agttcattac gtgaatggac tttcgatcgc gtactggccc acgctgatgc
     4921 gggtgaaagc tatatgtggg aatgtccgga ctttttcagc cttggcgatc agcattatct
     4981 gatgttttcc ccgcagggaa tgaatgccga gggatacagt taccgaaatc gctttcaaag
     5041 tggcgtaata cccggaatgt ggtcgccagg acgacttttt gcacaatccg ggcattttac
     5101 tgaacttgat aacgggcatg acttttatgc accacaaagc tttgtagcga aggatggtcg
     5161 gcgtattgtt atcggctgga tggatatgtg ggaatcgcca atgccctcaa aacgtgaagg
     5221 ctgggcaggc tgcatgacgc tggcgcgcga gctatcagag agcaatggca aacttctaca
     5281 acgcccggta cacgaagctg agtcgttacg ccagcagcat caatctatct ctccccgcac
     5341 aatcagcaat aaatatgttt tgcaggaaaa cgcgcaagca gttgagattc agttgcagtg
     5401 ggcgctgaag aacagtgatg ccgaacatta cggattacag ctcggcactg gaatgcggct
     5461 gtatattgat aaccaatctg agcgacttgt tttgtggcgg tattacccac acgagaattt
     5521 agacggctac cgtagtattc ccctcccgca gcgtgacacg ctcgccctaa ggatatttat
     5581 cgatacatca tccgtggaag tatttattaa cgacggggaa gcggtgatga gtagtcgaat
     5641 ctatccgcag ccagaagaac gggaactgtc gctttatgcc tcccacggag tggctgtggt
     5701 gcaacatgga gcactctggc aactgggtta acataatatc aggtggaaca acggatcaac
     5761 agcgggcaag ggatccgcgt cacacttccc ccttcacgac cttcaataat atgcaatgca
     5821 gcttcccgcc cgataatgtc atgtggaagc tgaattgtgg tcagcggcgg taaaaacaga
     5881 tgcccgacgc caaccagatt atcaaagccc attacggcga catcctgcgg gatacgtacc
     5941 cccttagcca ggagaacctg ataagccaca aaggctgcgc gatcgttacc acatatcaga
     6001 acatcaaaat ctggtttgcc cgatttgaag tggtcattga gtaaacttgc gagatcggtg
     6061 tagtgatcat cacctgttgc catgtgaaat tgtttcacct cagccagatc tcgtccagca
     6121 tcacgccagg cctgctcaaa tccctgccga cgataccctg ttgccaacgc actttccggt
     6181 agccagaagc ataacggttg acgatagccc gccgcgagca aatgctgtgt tgattcatat
     6241 tgtgcagtgt aatcatcagg gatataactg ggtaacgctg ggtcatccgc cacacagttc
     6301 gccaatacaa tattttcacc atacagagac tcaggtagcg tgatatgtcg cagccccatt
     6361 gtagtataga taatgccatc cggacggtgg gcaagcagct gacgtgccgc gcgggcagcg
     6421 tcatcttcag aaaaaatatt gattaaaaaa ctattccagc cgaactcgct ggcggtttgc
     6481 tcaatggcaa gcagaatatc aacagagaaa ggagtggtag cagtgtcctg cgccagcacg
     6541 gcgagagtcg acggcttacg tccttgagcg cgcatcttac gggcggaaag atcaggaaca
     6601 taattcaggg tctggattgc ctgcaatacg cggtcacgcg ttgcaggacg cacagattct
     6661 gcattatgca tcacccggga gactgtcatc atcgacactc ccgccaggcg tgcgacatcc
     6721 tttaatgaag ccatacccaa gccgtttgcc gtaaaacggg cactgtagca gaaacagacg
     6781 tcactggcga gatccaacga aattctcgac gccctatcac ccgacacggc aatacaataa
     6841 aaaataacaa taattgccgg ataattgtac ccaattccgc ctctgttctc gccaacgagt
     6901 gaccatatag gcaagtaaga aaaacatgcc aactggcatc gccaacctgg agaagaaaat
     6961 ataaatatgg atcattgtgc tgccgctgta tcccgacatc agcccggaaa ttattgcgat
     7021 tgctatcggt tcaggcgcga ttggctgcac gatcgttacg gactcgcttt tctggctggt
     7081 gaagcaatat tgcggcgcta cgctcaatga aacatttaaa tactatacga cagcgacatt
     7141 tatcgcttca gtcgtcgctc tggcgggcac attcctgctg tcatttatca tctaagcgca
     7201 aagagacgta ctatggaaaa cgctaaaatg aactcgctca tcgcccagta tccgttggta
     7261 aaggatctgg ttgctcttaa agaaaccacc tggtttaatc ctggcacgac ctcattggct
     7321 gaaggtttac cttatgttgg cctgaccgaa caggatgttc aggacgccca tgcgcgctta
     7381 tcccgttttg caccctatct ggcaaaagca tttcctgaaa ctgctgccac tggggggatt
     7441 attgaatcag aactggttgc cattccggct atgcaaaaac ggctggaaaa ggaatatcaa
     7501 caaccgatca gcgggcaact gttactgaaa aaagatagcc atttgcccat ttccggctcc
     7561 ataaaagcac gcggcgggat ttatgaagtc ctggcacatg cagaaaaact ggctctggaa
     7621 gcggggttgc tgacgcttga agatgactac agcaaactgc tttctccgga gtttaaacag
     7681 ttctttagcc agtacagcat tgctgtgggc tcaaccggaa atctggggtt atcaatcggc
     7741 attatgagcg cccgcattgg ctttaaggtg acagtgcata tgtctgctga tgcccgggca
     7801 tggaaaaaag cgaaactgcg cagccatggc gttacggtcg tggaatatga gcaagattat
     7861 ggtgttgccg tcgaggaagg acgtaaagca gcgcagtctg acccgaactg tttctttatt
     7921 gatgacgaga attcccgcac gttgttcctt gggtattccg tcgcaggcca gcgtcttaaa
     7981 gcgcaatttg ccgagcaagg tcgtattgtc gatgctgata accctctgtt tgtctatctg
     8041 ccgtgtggtg ttggcggtgg tcctggtggc gtcgcattcg gacttaagct ggcgtttggc
     8101 gatcatgttc actgcttttt tgccgaacca acgcactccc cttgtatgtt gttaggcgtc
     8161 catacaggat tacacgatca gatttctgtt caggatattg gtatcgacaa ccttaccgca
     8221 gcggatggcc ttgcagttgg tcgcgcatca ggctttgtcg ggcgggcaat ggagcgtctg
     8281 ctggatggct tctataccct tagcgatcaa accatgtatg acatgcttgg ctggctggcg
     8341 caggaagaag gtattcgtct tgaaccttcg gcactggcgg gtatggccgg acctcagcgc
     8401 gtgtgtgcat cagtaagtta ccaacagatg cacggtttca gcgcagaaca actgcgtaat
     8461 gccactcatc tggtgtgggc gacgggaggt ggaatggtgc cggaagaaga gatggagcaa
     8521 tatctggcaa aaggccgtta ataacgtttc aacgcagcat cgcaatcctt tccctgggtg
     8581 agcgatgctg ccgatggcgc agacttaaga tccccagtct tacccgctat aaccccctgc
     8641 ttttaccatc acccaacgcc tttcgctgta aacggcggtt tcgcaaacca aaccaaaacc
     8701 gtaagtaaga taaaagcaat agccgccata cggaaaatct catttgcaga aatagaaagt
     8761 gactgctggg ttatttcatt attaatttca ttaagaactc ctgaaagcga accatagtat
     8821 ttatccataa tttgtgacga tgaattaaac acggggttaa attgatcgat ggtttctgtc
     8881 aactgactat ggtgtaacga ttcgcgtcgt ccccacagcg tcattgtcaa cgacgtacca
     8941 actgatcctg acaaggtacg aaaaaaatta ctcatactcg aggcattggc aaatttatta
     9001 tctggcaagc ctgaaaacga aatcgttgtt aagggtaaaa agaaacaggc aacggcgaat
     9061 ccctgaaaaa actgcggcaa aatgatacct gtaaaatcaa tcgttggcat aaatgtcaca
     9121 gaacgccagt aatagcaaac cgcatacatc aaaaaactga atgtcaccaa cacccgcatg
     9181 tctattttgt tgccataacg tcctatcaaa ggtgaaatta atagtggcat gatgccgatg
     9241 ggcgcataag caagtccggc ccatatcgca ttatacccca tcgtttcctg gagtaactgc
     9301 ggcataagga cgatcgctcc agagtaaaat aaatacgcgc atgtgatact cacaatacca
     9361 atggtgaagt tacgggactt aaacaaactg agatcaagaa tcgggttctc tgaggtcgac
     9421 tcccaaatga ctaaagagat cagaaaaata actgatacta ctgttaatat tattattgta
     9481 ctcgagttga accaatccag atcgcgccct ttatcaagca taatttgcaa gccaccaaca
     9541 ccgagcacta acagggtcag tcctggtaga ttcattttga ccggtgaagt ctcagtttct
     9601 cttcctttaa gtaaggttaa gcataatgtc aggacgataa tccccatagg gacattgatt
     9661 aaaaatatcc aaccccagct aaagttatca caaatataac cgcccaatat cggcccacat
     9721 atcggagcga taatcacggt cattgaccat aatgccagag caaatgttct tttttctggt
     9781 ggataattcc ttaataataa actctgtgac agtggaatta acggccctgc cattaacccc
     9841 tgaacgactc taaaaaatat cagcacatca agattgatcg ataaactaca cattaaggaa
     9901 gacagcgaaa aaaaagtgac tgaaagtaaa aataacctta attcgcctat tctttgtgcc
     9961 agcctgccgg taacagggat cgcaatggca tttgctacgc caaacgaggt gataacccag
    10021 gtgccttcgt ctgttgatgc tcccagaaag ccagatattg tcggtattgc gacgttagaa
    10081 atagtggaat ccaacatttg cataaacgtc gctaatgaca atgcaatagt gacgcaccat
    10141 aacgtcccac cggttaatgg tgccggagtt gatttagtga ttgccattaa agttgtccat
    10201 tatgcgaaat aatgttgcta atttcttttt ctatcggact ggtatcgata actaaagcct
    10261 tactggtata agccggcatg gacgtcacgg ttgaagccag ctcaggcatc tcagcaatgt
    10321 cttcgttctt cgtatcaata gttgcagtca tcgataaacc aatgcgcaag gggtgttcca
    10381 tgagttcttt tggatcaaga gaaacttcaa ccggtacacg ctgaacgatt ttgatccagt
    10441 tccctgtcgc attttgtgca ggtaataagg agaacgcatt gccggttccc atattgatcc
    10501 ctgtcacccg gccatgaaac acaacattct caccataaag atcgctgata atattgaccg
    10561 attgaccaat ccgtacatcc gtgagttgtg tttctttaaa gttagcatta acccacattt
    10621 gacgtgccgg tacgacagcc attaacgatt gtccgggact cactgtttcg ccgacctgaa
    10681 cacttctctg ggcaatatag ccggtaaccg gactcttaat atccgtacgt ttaagcgcca
    10741 accaggcttc tttggttgca tccgcagctt caacgacttg tggctgacgg tttaatggtg
    10801 tgttcattac taaagcttta ttcgctttat aagcctggat agcggcattc aatgccgctt
    10861 tgctacttat taacgtatct ttggtatgct ccagcgtttc ttttgaaata accccctgct
    10921 tcgctaaagg cacacgacgg ttataatctt ctaaagattg ttgatactga atacgtgctg
    10981 aagcgacttc ggcactgtat tgtttatcct gtaagtacag tttattcgtt tgccgaacaa
    11041 tatttgccag attattttta gctttattga gtgcgatagt ggcatcagtt ttatccagtg
    11101 aaactaaaat gtcaccctgt cgaacgtagt tcgtatcttt atgattaacg acagtgacac
    11161 tacctgagac ttgtgcagaa attggatctg cattccccgt gacataggcg tcatctgtac
    11221 taatcatgtc ttctaattcc attgaccaat aggcataagc acctgaaaac gcaataaata
    11281 aaactatcgc caataaagaa aa
//
LOCUS       DBFIFM010000145        11274 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.145, whole genome shotgun sequence.
ACCESSION   DBFIFM010000145 DBFIFM010000000
VERSION     DBFIFM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.145"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..102
                     /locus_tag="N7C74_003406"
     CDS             <1..102
                     /locus_tag="N7C74_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HGU2433214.1"
                     /translation="LIFFTDYSHFMDRITLPSTDTARGKGKWKIITA"
     gene            81..185
                     /gene="speFL"
                     /locus_tag="N7C74_003407"
     CDS             81..185
                     /gene="speFL"
                     /locus_tag="N7C74_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HGU2433215.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            496..2694
                     /gene="speF"
                     /locus_tag="N7C74_003408"
     CDS             496..2694
                     /gene="speF"
                     /locus_tag="N7C74_003408"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HGU2433216.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERIPAEYLSRISGVFEHGEARKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFIPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2691..4010
                     /gene="potE"
                     /locus_tag="N7C74_003409"
     CDS             2691..4010
                     /gene="potE"
                     /locus_tag="N7C74_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HGU2433217.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSHVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4067..5707)
                     /gene="pgm"
                     /locus_tag="N7C74_003410"
     CDS             complement(4067..5707)
                     /gene="pgm"
                     /locus_tag="N7C74_003410"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HGU2433218.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKAVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5733..6278)
                     /gene="seqA"
                     /locus_tag="N7C74_003411"
     CDS             complement(5733..6278)
                     /gene="seqA"
                     /locus_tag="N7C74_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HGU2433219.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPIKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6463..7227
                     /gene="ybfF"
                     /locus_tag="N7C74_003412"
     CDS             6463..7227
                     /gene="ybfF"
                     /locus_tag="N7C74_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU2433220.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIAHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFSAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7367..7660
                     /gene="ybfE"
                     /locus_tag="N7C74_003413"
     CDS             7367..7660
                     /gene="ybfE"
                     /locus_tag="N7C74_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HGU2433221.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7800..8330
                     /gene="fldA"
                     /locus_tag="N7C74_003414"
     CDS             7800..8330
                     /gene="fldA"
                     /locus_tag="N7C74_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HGU2433222.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8540..8626
                     /gene="uof"
                     /locus_tag="N7C74_003415"
     CDS             8540..8626
                     /gene="uof"
                     /locus_tag="N7C74_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HGU2433223.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8619..9065
                     /gene="fur"
                     /locus_tag="N7C74_003416"
     CDS             8619..9065
                     /gene="fur"
                     /locus_tag="N7C74_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HGU2433224.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9149..9475)
                     /gene="chiQ"
                     /locus_tag="N7C74_003417"
     CDS             complement(9149..9475)
                     /gene="chiQ"
                     /locus_tag="N7C74_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HGU2433225.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9525..10930)
                     /gene="chiP"
                     /locus_tag="N7C74_003418"
                     /pseudo
     CDS             complement(9525..10930)
                     /gene="chiP"
                     /locus_tag="N7C74_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
BASE COUNT         2751 a         2777 c         2803 g         2943 t
ORIGIN      
        1 ttgatcttct tcacagatta tagccatttc atggatagaa taactctacc ttcaactgac
       61 acagcaagag gtaaaggtaa atggaaaata ataaccgctt aatgcctcat ataaggcgga
      121 caacccatat catgaagttt gcccatcgta atagttttga ctttcatttc tttaatgccc
      181 gctagtcttc tgactaaagg gcaccccagc gtacaggtct ccctgacttt aagcattaca
      241 ggttaatacc tgtatccctc ggtgctcata tactgctaac ccttttaaac tctacataat
      301 tcgagtcgca gcatatgcaa cttgaagtat gacgagtata gccagttacc gggctggtct
      361 gggttattgc atctgcaaaa agcaaactac tgatttattt atcagcggtg gagctttgct
      421 ttttttccgg cgtgatcgat ttctcctttg agaaattgag gacctgctat tacctgaaat
      481 aaagagatga acaaaatgtc agaattaaaa attgccgtta gtcgttcttg cccggattgt
      541 ttttccactc atcgtgcatg cgtgaatata gacgaaagta attatattga cgttgccgcc
      601 attattttat cagtcagtga tgttgaacgt ggaaaactcg atgaaataga cgctactggc
      661 tatgacattc ctgtttttat tgcaacggaa aatgaagaac gtataccagc agaatatctt
      721 tcacgtattt ctggtgtctt cgaacatgga gaagcacgca aagaatttta tggtcgtcag
      781 ttagaaaccg ctgccagcca ttatgaaact caactgcgcc cacctttctt tcgcgcactg
      841 gtcgattatg tcaatcaggg caacagcgca tttgactgcc ccggtcatca gggcggcgaa
      901 tttttccgtc gccatccggc ggggaatcag tttgtggaat actttggtga ggcgctgttc
      961 cgtgccgact tgtgcaacgc cgacgtagcg atgggcgatc tgctgattca cgaaggcgcg
     1021 ccatgcattg cacagcaaca tgcagcaaaa gtgtttaatg ccgataaaac ctacttcgtt
     1081 ttaaatggca cttcatcttc taacaaagtg gttttaaacg ccctgctgac accgggtgat
     1141 ctggtgctat ttgaccgcaa taaccataag tctaaccacc acggggcctt gctacaggct
     1201 ggtgcaacac cggtttatct ggaaacagca cgtaacccgt atggttttat cggtggtatt
     1261 gatgcacact gcttcgaaga aagttatctg cgtgaactga ttacagaagt tgcaccgcaa
     1321 cgggcaaaag aggcgcgtcc gttccgcctc gctgtgattc agctaggcac ctacgacggt
     1381 acgatttata acgcccgcca ggtggtggat aaaattggtc atctgtgtga ctacatcctg
     1441 tttgattccg catgggtcgg ctatgaacag tttattccga tgatggctga ctgttcgccg
     1501 ctgttgctgg atcttaatga gaacgatccg ggtattctgg ttacgcaatc cgtacataaa
     1561 cagcaggctg gtttttctca aacttcacaa atccataaaa aagacagcca catcaaaggg
     1621 caacagcgtt atgtaccgca caaacgcatg aacaacgcct ttatgatgca tgcctccacc
     1681 agcccgttct atccgctgtt tgccgcactg gatatcaacg ccaaaatgca tgaaggtgtc
     1741 agcggtcgta atatgtggat ggattgtgtg gtaaatggca ttaatgcccg caaactgatc
     1801 ctcgataact gtcagcatat tcgtccgttc atacctgaac tggtggatgg taaaccctgg
     1861 cagtcgtatg aaacagcgca aattgcggtt gatctgcgct tcttccagtt tgtaccaggt
     1921 gagcactggc attcttttga aggctatgca gagaatcaat actttgttga tccgtgcaaa
     1981 ctgttgctga caaccccagg tattgatgca cgtaacggcg aatatgaagc gttcggtgta
     2041 cccgcgacga ttcttgctaa cttcctgcgc gaaaatggcg tagtgccgga aaaatgcgat
     2101 cttaactcca tcctcttcct gctgactccg gcagaagata tggccaaact tcagcaactt
     2161 gttgccctgc tggtacgctt cgaaaaactg ctggaatctg atgcgccgct ggcggaagtg
     2221 ctaccttcca tctacaaaca gcatgaagag cgctacgccg gttataccct tcgtcagttg
     2281 tgccaggaaa tgcatgattt gtatgcccgc cacaacgtga aacaactgca aaaagagatg
     2341 ttccgtaagg aacacttccc acgcgtcagc atgaatccgc aagaagctaa ctacgcctat
     2401 ttacgcggtg aagtggagct ggttcgtctg ccggatgcag aaggccgtat cgctgccgaa
     2461 ggtgcgcttc cttatcctcc gggtgtgctg tgtgttgttc cgggtgaaat ctggggtggt
     2521 gctgttttgc gttacttcag cgctctggaa gaagggatca acctgctgcc aggttttgca
     2581 ccggagctgc agggtgtcta tatcgaagaa catgatggtc gtaagcaagt ttggtgctat
     2641 gtcatcaagc ctcgtgatgc gcaaagcacc ctgctgaaag gggaaaaatt atgagtcagg
     2701 ctaaatcgaa caaaatgggc gtcgttcagt taaccatact gacgatggtc aacatgatgg
     2761 gctccggtat catcatgctg ccgacaaagc ttgccgaagt tgggacaatc tcaattatct
     2821 cctggctggt gacagccgta ggctcaatgg cactggcatg ggcattcgca aaatgcggta
     2881 tgttcagccg taaatcaggc ggtatgggcg gttatgccga gtatgctttc ggtaaatccg
     2941 gtaactttat ggcgaactat acctacggcg tctcactgct gattgctaac gtcgcgattg
     3001 ctatttcggc ggttggttac ggcaccgaac tgctcggcgc gagtttgtcg ccagtgcaga
     3061 ttggtcttgc gaccatcggg gtgctgtgga tttgtaccgt ggctaacttt ggtggtgcgc
     3121 gcattaccgg gcaaatcagt agcattactg tgtggggagt cattattccg gtcgtcggtc
     3181 tgtgcattat tggctggttc tggtttagcc cgacgctgta cgttgattcc tggaatccgc
     3241 atcatgcacc gttcttcagt gcggtaggtt cttccatcgc tatgacgctg tgggcttttc
     3301 ttggtctgga gtctgcgtgt gcaaatactg atgtagtgga aaacccggaa cgtaatgtgc
     3361 caatcgcggt actcggcggt acgttaggtg cggcggtgat ttatatcgtc tccaccaacg
     3421 tgattgccgg gattgtgcca aatatggagc tggcaaattc aacggcacca tttggtctgg
     3481 ccttcgcgca gatgttcacg ccggaagtgg gtaaagtcat tatggcgctg atggtgatgt
     3541 cctgctgcgg ttcgctgctt ggctggcagt tcaccattgc ccaggtgttt aaatcttcat
     3601 ctgatgaagg ctacttccct aaaattttct cccatgtaac caaagtggat gcaccggtgc
     3661 agggaatgtt gaccattgtg attatccaga gcgggttggc gctgatgacg attagcccgt
     3721 cgctaaacag tcagttcaac gtgctggtta acctggccgt ggtgaccaat atcattccgt
     3781 atattctgtc aatggcggca ttagtcatta ttcagaaggt cgctaatgtg cccccctcaa
     3841 aagcgaaagt tgcaaacttt gttgcttttg ttggcgcgat gtacagcttt tatgcgctgt
     3901 actcatccgg ggaagaagcc atgctgtacg gttccatcgt gaccttcctt ggttggacac
     3961 tgtatggtct ggtatcacca cgctttgaac tgaaaaataa acacggttaa aaaagcaaag
     4021 taaaaaaggg cgatcttgcg accgcccttt ttcttattta atgtgtttac gcgtttttca
     4081 gaacttcgct aacaatctca accgcttctt tctcaatctg cttgcgatgt tcttcaccga
     4141 ggaagctttc gcagtagatc ttatatgcgt cttccgtgcc tgacggacgc gcggcgaacc
     4201 agccgttgtc agtcatcact ttcagaccgc caatagaagc accgttgccc ggagcagcag
     4261 tcaggcgcgc ggtgatcggg tcaccagcca gggtgctggc actcaccatt tccggagaca
     4321 gcttagacag cgccgctttt tgtgcggaag tcgcagctgc ctgcaaacgg ttgtagctcg
     4381 gcgcaccaaa gcgttttgcc agttcgttgt agtgttcctg cgggttctta ccggtgacag
     4441 cggtgatttc cgccgccagc agacacatga tgatgccgtc tttgtcggtg gaccacggcg
     4501 tgccgtcgaa acgcaggaag gaagcccctg cgctctcttc gccgccaaag ccgaagctgc
     4561 cgtcaaacag accatcaaca aaccatttga aacctaccgg gacttctacc agtttacggc
     4621 ccaagtcgtt gaccacacgg tcgatcatcg cagatgaaac cagcgtttta ccgacggcaa
     4681 cagctttgcc ccactgcgga cgatgctgga acaggtagtt gattgccacc gccaggtagt
     4741 ggttcggatt catcaaacct gccggagtga cgataccgtg acggtcataa tccgggtcgt
     4801 tagcaaacgc cagatcgaac ttatcacgca gtgccagcag gcccgccatc gcacactcgg
     4861 aggagcagtc catacggatc gcgccgtctt tatcaaggtg cataaagcgg aaggtttgat
     4921 cgacctgatc gttaacgatg gtcaggttga ggttgtaata ctcgccaata cgcttccagt
     4981 attcgatacc ggaaccgccc agcggatcaa cgccaagcgt caggcccgct ttctgaatcg
     5041 ccgccatatc aacgatatcg gccagccctt ccacaaacgg ctgcaccaga tcctgctctt
     5101 tcacatgacc ggatgccatc gcttcgtcga gggagatacg cttcacgcct ttcaggccat
     5161 cggccatcag tgcgttggct ctgtcttcca ccactttagt gacgttggta tcagccgggc
     5221 caccatttgg cggattgtac ttgataccac catcttccgg cgggttatgg gacggtgtaa
     5281 taacgatacc gtctgccagc gggccacctt ttttattgtg aaccaggatg gcattggaaa
     5341 cggcaggcgt cggggtgaag ccattgtttt cctgcacaat aacatctacg ccgttcgctg
     5401 ccagcacttc cagaacggaa ataaatgcag gttcggacag ggcgtgagta tctttaccca
     5461 catagcaagg gccagtgatg ccgtttttcg cacgttcttc agcaattgcc tgagcgatcg
     5521 ccagaatgtg cggctcgtta aagctgtggc gcgctgcact gccacggtga ccggaagtac
     5581 cgaatttcac cgcgtgctcc gcattccctg cttctggttt cagtacataa tattgcgccg
     5641 tcagttgggc gacgttaatc aaatcactct gttgtgcagg ttggcctgca cgattgtgga
     5701 ttgccattgc tttgtccttt gtctgcaacg ttttagatag ttccgcaaac cttctcaatc
     5761 aattccgccg ggaattgcat cgactgcatg atgtgttcga tcatgctgca tttacggccg
     5821 gtgttggtgt tggtgatcac ccaatacggc gtgcctggca catgtttcgg cttggtctga
     5881 ttaccatttt tcagcagcgt ttgttcatct gccgcaaagt aaacgcgtgt acgaccgtgc
     5941 aacgattccg ttgcttcggc aaacgcctgg gcgtcaagag aatatagtgt agacaacagc
     6001 agcataaagc gattgaccgc tcgcttttgc tctgcatatt catccgaaag cagaagttca
     6061 cgcattgcgc gaaccttgtc tttaatcgtt ttgatcggct tcgcttcgac gatagcaggt
     6121 gacgcaacgc gaacctcttt cgtcaccgga gcagcaggct gtgatgcggc ggaaaatttc
     6181 aacatacgcc gtaaaatgtc ggatgcgctc tcgccgatat gcttagtgtg gctggcaata
     6241 tagctgtaga gttcatcatc aacttcaatc gttttcatct taatccagtg cagtgtctta
     6301 gctgaataca agtcgccagg agtatatgaa taaatcccgg cagcggatag cgtcaagcct
     6361 ggcttgcaga aaaactcgga ataaacctta tttgttgcag ccctgtggca caatggtcaa
     6421 cataataccc taacccgaca acgcgaaaaa aagaactttg ccatgaaatt gaatatccgc
     6481 gcgcaaactg cacaaaacca gcacaataat tctcccatcg ttcttgtcca tggtctgttt
     6541 ggcagcctcg acaaccttgg cgtactggct cgcgatctgg taaacgatca caatatcatc
     6601 caggttgata tgcgtaacca cggtctttca ccgagagatc cggtaatgaa ttacccggcg
     6661 atggcgcagg atcttgttga tactctggat gcacagcaga tcgacaaagc aacatttatc
     6721 gctcactcca tgggcggtaa agcggtaatg gcacttactg cactggcccc cgatcgcatc
     6781 gataaactgg tggcgatcga tatcgcaccg gtcgactatc acgtacgccg tcatgatgag
     6841 attttctcgg ctatcaacgc ggtcagtgaa tcggacgcac aaactcgcca gcaagcagca
     6901 gcaataatgc gccagcatct taatgaagaa ggggtgattc agtttctgtt gaaatctttt
     6961 gttgacgggg agtggcgctt taacgtgccg gtattgtggg atcagtatcc gcatattgtg
     7021 ggttgggaga aaatcccggc atgggatcac cctgccctgt ttatccctgg tggtaattct
     7081 ccgtatgtta gcgagcagta ccgtgatgat ttactggcac aatttccaca ggcacgggcg
     7141 catgtgattg caggcgcggg tcactgggtc catgctgaaa aaccggatgc ggtattacgc
     7201 gctatccgtc gctatctcaa tgattaacgc aactgattaa aaacccagcg tccgcgtcgc
     7261 tttccgcgcg tgggcgttgg cgtgactcat cgcgctgatg tattatggcg cgctatccat
     7321 ccgggcagag gcctggctga ttgtttcccc cgaagtcacc aagatcatgg ccaaagaaca
     7381 aacggaccgt acgacattag atctgttcgc gcacgagcgt cgaccgggac gaccgaaaac
     7441 aaatccgctt tcgcgcgatg aacagctgcg tattaataaa cgcaaccaac taaaacgcga
     7501 caaagtacgt ggcctaaagc gtgtcgaact gaagctgaac gcggaagctg tcgaggcgct
     7561 gaacgagctg gcggaatcgc gcaatatgag ccgtagcgaa ctgatcgaag agatgttaat
     7621 gcagcaactg gcggcgctgc gtagtcaggg catcgtttaa atttccactt tcatgtagca
     7681 cagtgtgcag tcctgctcgt ttgctgattc cgcaagactg cctgttctgc tatgattgcc
     7741 tttatccgtg ggcaattttc cacccccatt tcaataagtt tcaagaggtt atttcactca
     7801 tggctatcac tggcatcttt ttcggcagcg acaccggtaa taccgaaaat atcgcaaaaa
     7861 tgattcaaaa acagcttggt aaagacgttg ccgatgtcca tgacattgca aaaagcagca
     7921 aagaagatct ggaagcttat gacattctgc tgctgggcat cccaacctgg tattacggcg
     7981 aagcgcagtg tgactgggat gacttcttcc cgactctcga agagattgat ttcaatggca
     8041 aactggttgc gctgtttggt tgtggtgacc aggaagatta cgccgaatat ttctgcgacg
     8101 cattgggcac catccgcgac atcattgaac cgcgcggtgc aaccatcgtt ggtcactggc
     8161 caactgcggg ctatcatttc gaagcatcaa aaggtctggc agatgacgac cactttgtcg
     8221 gtctggctat cgacgaagac cgtcagccgg aactgaccgc tgaacgtgta gaaaaatggg
     8281 ttaaacagat ttctgaagag ttgcatctcg acgaaattct caatgcctga tgtgatgcgg
     8341 cgtagactca tgtctacgcc gtattaatag ataatgccaa tcaaaataat tgctacaaat
     8401 ttgtaacttt tgccgttgta cctgtacaat gtcccggtgt tcaagtggcc ttgccgttgt
     8461 aaatgtaagc tgtgccacgt ttttattaac aatatttgcc agggacttgt ggttttcatt
     8521 taggcgtggc aattctataa tgatacgcat tatctcaaga gcaaattctg tcacttcttc
     8581 taatgaagtg aaccgcttag taacaggaca gattccgcat gactgataac aataccgccc
     8641 taaagaaagc tggcctgaaa gtaacgcttc ctcgtttaaa aatcctggaa gttcttcagg
     8701 agccggacaa ccatcacgtc agtgcggaag atttatacaa acgtctgatc gatatgggtg
     8761 aagaaattgg tctggctacg gtatatcgcg tactgaacca gtttgacgac gctggtatcg
     8821 tcacccgcca caattttgaa ggcggtaaat ccgtatttga actgacacag caacatcacc
     8881 acgatcacct gatctgcctc gactgcggca aggttatcga atttagtgat gattccatcg
     8941 aagcgcgtca gcgtgaaatt gccgcaaaac atggcattcg cctgactaac cacagtctct
     9001 atctttacgg tcactgcgcc gaaggcgatt gccgcgaaga tgagcacgcg cacgaaggca
     9061 aataagccag tctgaacgag aaaagccaac ctgcgggttg gcttttttat gcaagggaaa
     9121 taagaaaagc gagagttaca gctctcactt atttgttatt actgcgaatt tcctgccaga
     9181 ccttatcaca atcggctttc accgcctgat catttccggt ttgcgttgca cgcagacact
     9241 gctgataatc cagtacgcgg acactttcct ggtcggcaaa ctgctgatgt tgtttctctt
     9301 tcttcagcac gtttaacacg ctctggcagg cttcaatttt ttccggcgaa ccttgtgcgg
     9361 tgttgataca ggcgctgtat gcctctttca gacggctgtc ttcctgtggc gcggttgatt
     9421 gcgcacaagc taccagcccc gatgccatca tggcgatgag aatcagtttt ttcatagcaa
     9481 attcctcaaa cgtgcggcag gcacaaaacc tgccgcgtcg ggcatcagaa gatggtgaat
     9541 ggtgcgatta ccataaattt tacgtcacgc tcatcctgga agatgttgcc gtaaccgccg
     9601 ccccagcttg ggatgtcgga gtggttgtcg tattcggtga agtgcagttt gaacatcgtg
     9661 cctttggcgc gaccgtcctg aatggtgtag accgcatcca ggctgtatgc agactcttca
     9721 atagtccggt ttttgtcgta gtacgcatcc ggattgctct gccaggtcgc aggtttagca
     9781 tcccatgcgt aaacgtagga agcgccgatg gcgaagcctg gaagattcca gtttttcagg
     9841 tcatacatcg caccgaagaa gaccgctttt tcgccgttgg cgttgaagtc agaacggtta
     9901 tcccaccaga tatccaggcg accgttcgag gaagcgtagg ttggagtcat acgttgcagg
     9961 aagtatccct gctgaccgtc agctttaacc caggtgcctt cgaggcgcaa atccactacg
    10021 tcagccgccc ggtaaccaaa ggtcaacgct tgcagccagg cggtgccgtc ataaagatcg
    10081 ttgacgctgc gatcgtcaac tttatcgcgc gtaccgtaga actggtagct ggtggttaac
    10141 gggctaccgg cgatatcaaa tttgtagctg gctttggcaa agtattgatc gatataccct
    10201 tccgcctgac caaatgccgc ttccagtacg aagttatttt tgaagtcgta tttcgcccca
    10261 agggagtgca gataatcaac tttggtggtt ttatcgttct gataaaactc atccatttcc
    10321 agatgccacg gtgctttgta ttcgttggtc cacatgtagg agaaactcaa cgcaccagca
    10381 tcgccgtaat caaaattcgc cccggcctcc gcaccctgat aagtacccgg cataaagctc
    10441 cagtgcggcg ctaacagcgt ttgaccagtt ggctgaatat aacctgccct cgccacaccg
    10501 gaccgtattt aaatttggcc gctgctttat acaggcttat accgctttta tcgccggacc
    10561 agtcttcgtc ataggcttta ttactttttg aaaacgcgat ttcgttcggg tgagagctgt
    10621 cgccgttttc cgccatttca atcgccgtaa acgcggcaat atcaaggccg aacatatcag
    10681 cagcataacc ggactgaaaa tcgaggttgg cgttccaggt ggagtgagaa aggttggttt
    10741 tgtatttatc gccgtcggta acatctttac ggtcgcgttc acgctgccag taatagatac
    10801 cgccggttaa ggttgaatcg tcaatgaatc cttcggcgcg agcctccggc attgccataa
    10861 agcccgacat tgctgtaaca ccggcgatag ccagcgccag cgtactacgt ttgccactaa
    10921 acgtacgcat gggttaatcc tctttgacgt ataaattgct gcaccacagg tgaacagcga
    10981 aaaaaataaa aattaaaaaa gtagcgttga gaaaagtctc gctgactaca ggaacagact
    11041 atttttcgcg acgcgaatta tcaatctctt tctgtaacca gaatgtaaga acatgagcaa
    11101 ccgcacatat tttgatgatt tttgttacaa ggcttaagtt tgcggttgaa aggcttgcag
    11161 aagaataaaa gcgtgaaatt ccagcaagat gaaagcggtt acattttatc taattatgtt
    11221 ttgtgttttt aaatttattt tatggataaa ttgcgaagcg gaattaattc gcga
//
LOCUS       DBFIFM010000146        11231 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.146, whole genome shotgun sequence.
ACCESSION   DBFIFM010000146 DBFIFM010000000
VERSION     DBFIFM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.146"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            111..251
                     /locus_tag="N7C74_003419"
                     /pseudo
     CDS             111..251
                     /locus_tag="N7C74_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(276..590)
                     /gene="fliE"
                     /locus_tag="N7C74_003420"
     CDS             complement(276..590)
                     /gene="fliE"
                     /locus_tag="N7C74_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HGU2433226.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            805..2463
                     /gene="fliF"
                     /locus_tag="N7C74_003421"
     CDS             805..2463
                     /gene="fliF"
                     /locus_tag="N7C74_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HGU2433227.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSWRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2456..3451
                     /gene="fliG"
                     /locus_tag="N7C74_003422"
     CDS             2456..3451
                     /gene="fliG"
                     /locus_tag="N7C74_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HGU2433228.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3444..4130
                     /gene="fliH"
                     /locus_tag="N7C74_003423"
     CDS             3444..4130
                     /gene="fliH"
                     /locus_tag="N7C74_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HGU2433229.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            4192..5565
                     /gene="fliI"
                     /locus_tag="N7C74_003424"
     CDS             4192..5565
                     /gene="fliI"
                     /locus_tag="N7C74_003424"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HGU2433230.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            5584..6027
                     /gene="fliJ"
                     /locus_tag="N7C74_003425"
     CDS             5584..6027
                     /gene="fliJ"
                     /locus_tag="N7C74_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HGU2433231.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            6024..7151
                     /gene="fliK"
                     /locus_tag="N7C74_003426"
     CDS             6024..7151
                     /gene="fliK"
                     /locus_tag="N7C74_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HGU2433232.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDIVSDAQQANLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMALAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPQVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSSHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            7256..7720
                     /gene="fliL"
                     /locus_tag="N7C74_003427"
     CDS             7256..7720
                     /gene="fliL"
                     /locus_tag="N7C74_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HGU2433233.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            7725..8729
                     /gene="fliM"
                     /locus_tag="N7C74_003428"
     CDS             7725..8729
                     /gene="fliM"
                     /locus_tag="N7C74_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HGU2433234.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            8726..9139
                     /gene="fliN"
                     /locus_tag="N7C74_003429"
     CDS             8726..9139
                     /gene="fliN"
                     /locus_tag="N7C74_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HGU2433235.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            9142..9507
                     /gene="fliO"
                     /locus_tag="N7C74_003430"
     CDS             9142..9507
                     /gene="fliO"
                     /locus_tag="N7C74_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HGU2433236.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            9507..10244
                     /gene="fliP"
                     /locus_tag="N7C74_003431"
     CDS             9507..10244
                     /gene="fliP"
                     /locus_tag="N7C74_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HGU2433237.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10254..10523
                     /gene="fliQ"
                     /locus_tag="N7C74_003432"
     CDS             10254..10523
                     /gene="fliQ"
                     /locus_tag="N7C74_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HGU2433238.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            10531..>11231
                     /gene="fliR"
                     /locus_tag="N7C74_003433"
     CDS             10531..>11231
                     /gene="fliR"
                     /locus_tag="N7C74_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HGU2433239.1"
                     /translation="MLQVTSEQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMP"
BASE COUNT         2647 a         3005 c         3090 g         2489 t
ORIGIN      
        1 tcgctcatat ccatagcccg gaacatgaat aaaaacatca acaaagataa aattatccag
       61 aggttactgt tgcgtctgtg gtcgtaacaa catccgacgt ggtgatgtgt caaatgagac
      121 actctgatgt tcagggggtt tatcgtgtat acggatcgtg gatggcagaa aactttcagg
      181 atcaggtatc catttcaaac cagataatga gtaagtttgc cccatccatg ccccacgcgg
      241 tgagatcgga tgtcattaac aacaggttac acaacctata cctgcatgct cattacttcc
      301 tgatatgccg ccaccagctt attacgcacc tgaatcccca tttgcataga aactgaggct
      361 ttttgcatat cggtcatcac atcgtttaac gccacgccgg gttcaccgag agtgaatttt
      421 tctgcctgcg tgcgggcggc tgtttgtgta tcgcttatgc gatcgagcgc ggcgtgcagc
      481 tgcccggcaa aactaatggt cggttgcggc agtgattcct gcgcacgcgc actcatcgcc
      541 gtagcctgta actggctgat aaccccttca atcccctgta tcgctgacat tttcatctcc
      601 tggataattt ctggtagcaa agcctaccag taagtcaata agacaaaggc gctaaatagc
      661 aacaaaaaaa cgggtttatt ggcggataga aaaaaacgaa agcacaaata atgggagcgt
      721 caatttttcg agtttgctga cccgggagtg agtcttgttc cactttgcca ataacgccgt
      781 ccataatcag ccacgaggtg cgcgatgaat gcgactgcag cccagacaaa atctcttgag
      841 tggcttaatc gcctgcgtgc gaatccgaaa attccattga ttgttgccgg ttccgcggca
      901 gtggcggtca tggtcgcact gatcctgtgg gcgaaagccc ccgactaccg cacattattc
      961 agcaatcttt ccgatcagga tggtggcgca attgtcagcc aactgacgca aatgaatatt
     1021 ccttaccgct tcagcgaagc cagcggcgct attgaagttc cggcagataa agttcacgag
     1081 ctgcgcctgc gcctggcgca acaaggtttg cccaaaggcg gcgcggtcgg tttcgaacta
     1141 ctcgatcagg aaaagtttgg tatcagccag ttcagcgaac aggtgaatta tcagcgggcg
     1201 ctggaaggcg agctttctcg taccatcgaa actatcggcc cggtaaaagg ggcgcgcgta
     1261 catctggcaa tgccgaaacc gtctttattc gtccgtgaac aaaaatcccc ttctgcatcg
     1321 gtgacggtaa atctgttacc cggccgcgca ctcgatgaag ggcaaattag cgccattgtg
     1381 catctggttt ccagcgccgt tgctggtctg ccgccgggaa acgtcacgct ggtggatcag
     1441 ggcggacatc tgttaaccca gtccaatacc agctggcgcg atcttaatga cgctcaattg
     1501 aaatatgcca gcgatgtcga aggccgtatt cagcggcgta ttgaagcaat cctgtcgcct
     1561 attgttggta acggtaatat tcacgcccag gtcacggcgc aactggactt cgccagtaaa
     1621 gaacaaacgg aagaacagta tcgccctaat ggtgatgaat ctcaggcggc gcttcgttca
     1681 cgccagctta atgagagcga gcaaagcggt tccggttatc cgggcggcgt accgggggcg
     1741 ttgtcgaatc aaccggctcc tgcgaataac gcgccaatca gcacgcctcc ggcaaatcaa
     1801 aataaccgcc agcagcaggc gagcaccacc agcaatagtg ggccgcgtag cacacagcgg
     1861 aatgaaacca gtaactacga agtcgatcgc actattcgtc ataccaaaat gaacgtgggc
     1921 gatgtgcaac gtctgtcagt cgcggtagtg gtgaattaca aaaccttgcc agacggtaaa
     1981 ccattgcctc tcagcaacga acagatgaaa caaattgaag atctgacccg cgaggcgatg
     2041 ggcttttctg aaaaacgcgg tgactcgctc aatgtcgtta actcgccgtt caatagtagt
     2101 gacgaaagcg gcggagaact gccgttctgg caacagcaag cgtttatcga tcagttactg
     2161 gctgccggtc gctggttgct ggtactgctg gtggcgtggc tgctgtggcg taaagcggta
     2221 cgcccgcagc taacacgtcg cgctgaggcg atgaaagctg tacagcaaca ggcgcaggcc
     2281 cgcgaggaag tggaagatgc ggtggaagtc cgcctgagca aagacgaaca actccaacaa
     2341 cggcgcgcta accaacgtct gggggcagaa gtcatgagcc agcgtatccg tgaaatgtct
     2401 gataacgatc cgcgcgtggt ggcgctggtc attcgccagt ggataaataa cgatcatgag
     2461 taacctgaca ggcaccgata aaagcgtcat cctgctgatg accattggcg aagaccgggc
     2521 ggcagaagtg ttcaagcacc tctcccagcg cgaggtgcaa accctgagcg ctgcaatggc
     2581 gaacgtcacg cagatctcca acaagcagct aaccgatgtg ctggcggagt ttgagcaaga
     2641 agctgaacag tttgccgcac tgaatatcaa cgccaacgat tatctgcgtt cggtattggt
     2701 caaagctctg ggtgaggaac gtgccgccag cctgctggaa gatattctcg aaactcgcga
     2761 taccgccagc ggtattgaaa cgctcaactt tatggagccg cagagcgccg ccgatctgat
     2821 tcgtgatgag catccgcaaa ttatcgccac cattctggtg catctgaagc gcgcccaggc
     2881 cgccgatatt ctggcgttgt tcgatgaacg tctgcgccac gacgtgatgt tgcgtatcgc
     2941 cacctttggc ggcgtgcagc cagccgcgct ggcggaactg accgaagtac tgaatggctt
     3001 gctcgacggt cagaatctca agcgcagcaa aatgggcggc gtgagaacgg cagccgaaat
     3061 tatcaacctg atgaaaactc agcaggaaga agccgttatt accgccgtgc gtgaattcga
     3121 cggcgagctg gcgcagaaaa tcatcgacga gatgttcctg ttcgaaaatc tggtggatgt
     3181 cgacgatcgc agcattcagc gtctgttgca ggaagtggat tccgaatcgc tgttgatcgc
     3241 gctgaaaggg gccgagcagc cactgcgcga gaaattcctg cgcaatatgt cgcagcgtgc
     3301 cgccgatatt ctgcgcgacg atctcgccaa ccgtggtccg gtgcgtctgt cgcaggtgga
     3361 aaacgaacag aaagcgattc tgctgattgt gcgccgcctt gccgaaactg gcgagatggt
     3421 aattggcagc ggcgaggata cctatgtctg ataatctgcc ctggaaaacc tggacgccgg
     3481 acgatctcgc gccaccacag gcagagtttg tgcccatggt cgagtcggaa gaaaccatca
     3541 ttgaagaggc tgaacccagc cttgagcagc aactggcgca actgcaaatg caggcccatg
     3601 agcaaggtta tcaggcgggt attgccgaag gtcgccagca aggtcataag cagggctatc
     3661 aggaaggact ggcccagggg ctggagcaag gtctggcaga ggcgaagtct caacaagcgc
     3721 caattcatgc ccggatgcag caactggtca gcgaatttca aactaccctt gatgcacttg
     3781 atagtgtgat agcgtcgcgc ctgatgcaga tggcgctgga ggcggcacgt caggtcatcg
     3841 gtcagacgcc aacggtggat aactcggcac tgatcaaaca gatccaacag ttgttgcagc
     3901 aagaaccgtt attcagcggt aaaccacagc tgcgcgtgca cccggatgat ctgcaacgtg
     3961 tggatgatat gctcggcgct accttaagtt tgcatggctg gcgcttgcgg ggcgatccca
     4021 ccctccatcc tggcggctgt aaagtctccg ccgatgaagg cgatctcgac gccagtgtcg
     4081 ccactcgctg gcaagaactc tgccgtctgg cagcaccagg agtggtgtaa tgaccacgcg
     4141 cctgactcgc tggcaagaac tctgccgtct ggcagcacca ggagtggtgt aatgaccacg
     4201 cgcctgactc gctggctaac cacgctggat aactttgaag ccaaaatggc gcagttgcct
     4261 gcggtacgtc gctacgggcg attaacccgc gctaccgggc tggtgctgga agccaccgga
     4321 ttacaattgc cgctcggcgc aacctgtgtc attgaacgcc agaacggcag cgaaacgcac
     4381 gaagtagaaa gcgaagtcgt tggctttaac ggtcaacggc tgtttttaat gccgctggag
     4441 gaagtggaag gtgtcctgcc cggcgcgcgg gtttatgcca aaaacatttc ggctgaagga
     4501 ctgcaaagcg gcaagcagtt gccgctcggt ccggcgttat taggtcgcgt tctggacggc
     4561 agcggtaaac cgctcgatgg cctgccctcc cccgatacga cggaaaccgg tgcgctgatt
     4621 accccgccat ttaacccgtt gcaacgtaca ccgattgaac atgtgctgga caccggcgtg
     4681 cgcccaatca atgccctgct taccgttggg cgtgggcagc gtatggggct gtttgccggg
     4741 tccggcgttg gtaaaagtgt gctgctgggg atgatggcac gttacacccg cgccgatgtc
     4801 attgtcgtgg gtttgattgg tgaacgtggg cgcgaagtaa aagattttat tgagaatatc
     4861 ctcggtgccg aagggcgtgc acgatcagtg gtgattgccg ctccggcgga tgtgtctccg
     4921 ctcctgcgaa tgcaaggtgc cgcctatgcc acgcgcattg ccgaagattt tcgcgatcgt
     4981 ggtcagcatg tgttgctgat tatggactcc ctcacccgct acgcgatggc ccagcgtgaa
     5041 attgcgctgg cgattggcga accacccgcc accaaaggtt atccaccgtc ggtgtttgcc
     5101 aaattaccgg cactggtcga gcgtgccgga aatggcatta gcggcggcgg ctcgattacc
     5161 gcgttttata ccgtgcttac tgaaggcgat gaccagcagg accccattgc agactccgcg
     5221 cgggcgatcc tcgacggtca cattgtgctg tctcgccgac tggcggaagc cgggcactat
     5281 ccggctatcg atattgaagc gtcgatcagc cgcgcaatga cggcgttgat cagtgagcaa
     5341 cattacgcgc gagtgcgcac cttcaaacag ctgttgtcga gttttcagcg taaccgcgat
     5401 ctggttagcg tcggcgcgta tgccaaaggc agcgatccga tgctcgacaa agccatcgcc
     5461 ctgtggccgc agctggaggg ctatttgcaa caaggcattt ttgaacgcgc ggactgggaa
     5521 gcgtctctcc aggggctgga gcgtattttc ccgacagtgt cataacccag gagataacgg
     5581 cagatggcag aacatggcgc gctggcgacc ctgaaagatc tggcagaaaa agaggtagag
     5641 gatgccgcgc gcctgctggg tgaaatgcgt cgcgggtgtc agcaggcgga agaacagctc
     5701 aaaatgctga tcgattatca gaatgaatat cgcaataacc tcaacagcga tatgagtgcc
     5761 gggatgacca gcaaccgctg gatcaactat cagcagttta tccagacgct ggaaaaagcc
     5821 attactcagc atcgccagca acttaatcag tggacgcaga aagttgacat tgccctgaac
     5881 agctggcgag aaaagaaaca acgtttgcag gcctggcaga cactgcagga acggcaatcc
     5941 acggcggcac tgcttgcaga aaaccgcctc gatcagaaaa agatggatga gttcgcccag
     6001 cgcgccgcca tgaggaaacc tgaatgattc gcttagcgcc cttgattacc gccgacgttg
     6061 acaccaccac attgcctggc ggcaaagcca gcgatgctgc acaagatttt ctcgcgttgt
     6121 tgagcgaagc attagcaggc gagacaacta ccgacaaagc ggccccccag ttgctggtgg
     6181 caacagataa gcccacgaca aaaggcgagc cgctgatcag cgatattgtt tccgacgcgc
     6241 aacaagctaa tttactgatc cctgtggatg aaacaccgcc tgtcatcaac gacgaacaat
     6301 ccacatcaac accgttaacc accgctcaga cgatggcgtt ggctgcggtg gctgacaaaa
     6361 atacgacaaa agacgaaaaa gcggatgatc tgaatgaaga cgtcaccgca agcctgagcg
     6421 ccctttttgc gatgttgccg ggttttgaca atacgcccaa agtgactgat gtgccgtcaa
     6481 ccgtgttacc ggcagagaaa ccaacgctct tcacaaaact gacttctgcg caactcacaa
     6541 cagcacagcc tgatgatgcc cccggcacac cagctcagcc attaacaccg caggtagcag
     6601 aagcccagag taaagcggaa gtcatcagca cgccttcgcc ggtgactgct gccgccagcc
     6661 cgctaatcac tccacaccag acacagccac tgcccaccgt cgccgcgcct gttttgagtg
     6721 caccgctggg ttctcacgaa tggcaacaat cattaagcca gcatatttcg ctgttcaccc
     6781 gccaggggca acaaagtgca gagttgcgtc tgcacccgca ggatttaggt gaagtgcaaa
     6841 tctccctcaa agtggatgat aaccaggcgc aaatccagat ggtttcatcg catcagcatg
     6901 tacgcgccgc cctggaagca gcgctgccgg tactgcgtac gcagctggcc gaaagtggca
     6961 ttcagttagg gcaaagcaac atcagtggcg aaagctttag tggtcagcag caggccgctt
     7021 cccaacaaca gcaaagccaa cgcacagtaa accatgaacc tctggcgggg gaagacgacg
     7081 atacgcttcc ggttcccgtc tctttacaag ggcgcgtaac aggcaacagc ggcgttgata
     7141 ttttcgccta acgtcagagg tagcaccgta atccgcgtct tttccccgct ttgttgcgct
     7201 caagacgcag gataattagc cgataagcag tagcgacaca ggaagaccgc aacacatgac
     7261 tgattacgcg ataagcaaga aaagcaagcg atcgctttgg atcccgattc tggtattcat
     7321 taccctcgcg gcctgtgcca gcgcaggtta cagctactgg cattcgcatc aggttgctgc
     7381 tgacgacaaa gcgcagcaac gcgtcgtgcc ctccccggtc ttctatgcgc tggatacctt
     7441 tacggtcaat ctgggcgatg cggatcgcgt gctttatatc ggcataaccc tgcgcctgaa
     7501 agatgaagct acccgctcgc ggctgagtga gtatttgccg gaagtccgta gccgtttgct
     7561 gttactgttt tcgcgtcagg atgctgccgt actggcgaca gaagaaggca agaaaaacct
     7621 gattgccgag attaaaacca cactttccac cccgcttgtt gccgggcaac cgaaacagga
     7681 tgtcaccgac gtgctgtata ccgcttttat tctgcgataa cgacatgggc gatagtattc
     7741 tttctcaagc tgaaattgat gcgctgttga atggtgacag cgaagtcaaa gacgaaccga
     7801 cagccagtgt tagcggcgaa agtgacattc gtccgtacga tccgaatacc caacgacggg
     7861 ttgtgcgcga acgtttgcag gcgctggaaa tcattaatga gcgctttgcc cgccattttc
     7921 gtatggggct gttcaacctg ctgcgtcgta gcccggatat aaccgtcggg gccatccgca
     7981 ttcagccgta ccatgaattt gcccgcaacc tgccggtgcc gaccaacctg aaccttatcc
     8041 atctaaaacc gctgcgcggc actgggctgg tggtgttctc accgagtctg gtgtttatcg
     8101 ccgtggataa cctgtttggc ggcgatggac gcttcccgac caaagtggaa ggtcgcgagt
     8161 ttacccatac cgaacagcgc gtcatcaacc gcatgttgaa actggcgctt gaaggctata
     8221 gcgacgcatg gaaggcgatt aatccgctgg aagttgagta cgtgcgttcg gaaatgcagg
     8281 tgaaatttac caatatcacc acctcgccga acgacattgt ggttaacacg ccgttccatg
     8341 tggagattgg caacctgacc ggcgaattta atatctgcct gccattcagc atgatcgagc
     8401 cgctacggga attgttggtt aacccgccgc tggaaaactc gcgtaatgaa gatcagaact
     8461 ggcgcgataa cctggtgcgc caggtgcagc attcacagct ggagctggtc gccaactttg
     8521 ccgatatctc gctacgcctg tcgcagattt taaaactgaa acccggtgac gtcctgccga
     8581 tagaaaaacc cgaccgcatc atcgcccatg ttgacggcgt cccggtgctg accagtcagt
     8641 acggcactct caacggtcag tatgcgttac ggatagaaca tttgattaac ccgattttaa
     8701 attctctgaa cgaggaacag cccaaatgag tgacatgaat aatccggccg atgacaacaa
     8761 cggcgcaatg gacgatctgt gggctgaagc gttgagcgaa caaaaatcaa ccagcagcaa
     8821 aagcgctgcc gacgcggtgt tccagcaatt tggcggtggt gatgtcagcg gaacgttgca
     8881 ggatatcgac ctgattatgg atattccggt caagctgacc gtcgagctgg gccgcacacg
     8941 aatgaccatc aaagagctgt tgcgtctgac gcaagggtcc gtcgtggcgc tggacggtct
     9001 ggcgggcgaa ccactggata ttctgatcaa cggttattta atcgcccagg gcgaagtggt
     9061 ggtcgttgcc gataaatatg gcgtgcggat caccgatatc attactccgt ctgagcgaat
     9121 gcgccgcctg agccgttagt gatgaataac cacgctactg tgcaatcttc cgcgccggtt
     9181 tctgctgcgc cactgctgca ggtgagcggc gcactcatcg ccattattgc cctgatcctc
     9241 gctgctgcct ggctggtaaa acggttggga tttgccccga aacgcactgg cgttaacggt
     9301 ctgaaaatta gcgccagtgc ttcgctgggc gcgcgtgaac gggttgtggt ggtcgatgtg
     9361 gaagatgcac ggctggtgct cggcgttacc gcagggcaaa tcaatctgct gcataaactt
     9421 cccccttcag caccaacaga agagataccg cagaccgatt ttcagtcggt catgaaaaat
     9481 ttgcttaagc gtagcgggag atcctgatgc gtcgtttatt gtctgtcgca cctgtccttc
     9541 tctggctgat tacgcccctc gccttcgcgc aactgccggg tatcaccagc cagccgctgc
     9601 ctggcggtgg acaaagctgg tcgctcccgg tgcagacgct ggtgttcatc acctcgttga
     9661 cgtttattcc ggcaatttta ctgatgatga ccagtttcac ccgcatcatc attgtttttg
     9721 gtttattgcg taacgcgctg ggaacaccct ccgcgccacc taaccaggta ttgctggggc
     9781 tggcactgtt tttgaccttt tttattatgt caccggtgat cgacaaaatt tatgtcgatg
     9841 cgtaccagcc attcagcgaa gagaaaatat caatgcagga ggcgctggaa aaaggggcgc
     9901 agccgctgcg tgagtttatg ctgcgtcaga cccgtgaggc agatttaggg ttgtttgcca
     9961 gactggcgaa taccggcccg ttgcagggac ctgaagccgt gccgatgcgc attttgctcc
    10021 cggcctacgt gaccagcgag ttgaaaaccg catttcagat aggcttcacg attttcatcc
    10081 cttttttgat tatcgacctg gtgatagcca gcgtgttgat ggcattgggg atgatgatgg
    10141 ttccccccgc caccattgct ctgcccttta aactgatgct gtttgtactg gtggatggct
    10201 ggcaattgct ggtcggttcg ctggcgcaga gcttttacag ctagagaggc aaaatgacac
    10261 ctgaatcggt catgatgatg gggactgaag cgatgaaagt cgcgctggca ctggctgccc
    10321 cgctattgtt ggtagcgttg gtcacgggcc ttatcatcag tattttgcag gccgccacgc
    10381 agattaacga aatgacgctg tcgtttattc cgaaaatcat cgccgtattt atcgccatta
    10441 ttattgccgg accgtggatg ctcaatctgt tgctggatta cgtccgcacc ttgttcacta
    10501 acctgccgta tatcatcggg tagccgtact atgttgcagg tgacaagcga acaatggcta
    10561 tcctggttaa gcctgtactt ctggccgtta ctgcgcgtgc tggcgctgat ctccaccgcg
    10621 ccgattctga gcgaacgcag cgtaccgaaa cgggtaaaac tgggtctggc aatgatgatc
    10681 acgttcgcca ttgccccatc attacctgcc aacgatgttc ctgttttttc gttctttgct
    10741 ctgtggctgg ccgtgcagca gatcctgatc ggcattgcgc ttggttttac catgcaattt
    10801 gcctttgccg ctgtgcgaac cgctggcgaa attatcggtc tgcaaatggg gctgtcattt
    10861 gcgacgtttg tcgatccggc cagccatctt aatatgcccg ttttagcgcg tatcatggat
    10921 atgctggcgt tactgctgtt cctgacattt aacggtcatt tatggttgat ttcactgctg
    10981 gtcgatacct ttcacaccct gccgattggt ggcgaaccgt tgaacagcaa tgcgtttctg
    11041 gcactcacca aagcagggag tttgattttc cttaacgggc tgatgctggc gttaccgctc
    11101 attactctgc tgctgacact gaatctggca ttaggtttac ttaatcgtat ggccccgcaa
    11161 ttatccattt ttgttattgg atttccatta actctgactg tcggcatctc tttaatggcg
    11221 gcattaatgc c
//
LOCUS       DBFIFM010000147        11214 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.147, whole genome shotgun sequence.
ACCESSION   DBFIFM010000147 DBFIFM010000000
VERSION     DBFIFM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.147"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..422)
                     /gene="gatA"
                     /locus_tag="N7C74_003434"
     CDS             complement(<1..422)
                     /gene="gatA"
                     /locus_tag="N7C74_003434"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HGU2433240.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEY"
     gene            complement(432..1694)
                     /gene="gatZ"
                     /locus_tag="N7C74_003435"
     CDS             complement(432..1694)
                     /gene="gatZ"
                     /locus_tag="N7C74_003435"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HGU2433241.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENVDAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(1723..2577)
                     /gene="gatY"
                     /locus_tag="N7C74_003436"
     CDS             complement(1723..2577)
                     /gene="gatY"
                     /locus_tag="N7C74_003436"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HGU2433242.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKHYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(2876..3928)
                     /gene="fbaB"
                     /locus_tag="N7C74_003437"
     CDS             complement(2876..3928)
                     /gene="fbaB"
                     /locus_tag="N7C74_003437"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HGU2433243.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            4185..5462
                     /locus_tag="N7C74_003438"
     CDS             4185..5462
                     /locus_tag="N7C74_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HGU2433244.1"
                     /translation="MKTTTKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFTGAALMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            5459..6463
                     /locus_tag="N7C74_003439"
     CDS             5459..6463
                     /locus_tag="N7C74_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HGU2433245.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPNLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFINDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPALKAELDAVNQLDFNRYATALAKYRQQREAA
                     "
     gene            6460..7425
                     /locus_tag="N7C74_003440"
     CDS             6460..7425
                     /locus_tag="N7C74_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HGU2433246.1"
                     /translation="MSGARLHTLLPELTTRQPVMVIGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPEALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFATPLIIRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(7399..8145)
                     /locus_tag="N7C74_003441"
     CDS             complement(7399..8145)
                     /locus_tag="N7C74_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU2433247.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8197..9015)
                     /locus_tag="N7C74_003442"
     CDS             complement(8197..9015)
                     /locus_tag="N7C74_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HGU2433248.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIHIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPVIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGSVDFNVFNGTEEELQAFVDGIKETP"
     gene            complement(9080..9880)
                     /gene="thiD"
                     /locus_tag="N7C74_003443"
     CDS             complement(9080..9880)
                     /gene="thiD"
                     /locus_tag="N7C74_003443"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HGU2433249.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRALLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(9877..10665)
                     /gene="thiM"
                     /locus_tag="N7C74_003444"
     CDS             complement(9877..10665)
                     /gene="thiM"
                     /locus_tag="N7C74_003444"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HGU2433250.1"
                     /translation="MQVDLLSSAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGVANGGRGVDTTDAAVNAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(10700..10796)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10999..>11214
                     /locus_tag="N7C74_003445"
     CDS             10999..>11214
                     /locus_tag="N7C74_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HGU2433251.1"
                     /translation="MTETQIYENIKQAISSAPRNSQTMEMHLQMIKYADHLKNVTAKE
                     FCEGVGLKASFATEFSKMRNLTERLKAA"
BASE COUNT         2663 a         2915 c         2819 g         2817 t
ORIGIN      
        1 aagtattcct ttaactgagt ttcaggaaga gtgattagtg tctcaacgat atcgggctgt
       61 tgtaacttgc caaataaaca gcgtaaaagt ttcaattgct gctgcggatt ttccacaatc
      121 aacgcaataa ccaacgatac cgccacgtca ttatcatcat ccgcttgctg aaaatgaact
      181 ttatttgttg gccttaacag ataaatggct gacgacttag catgaatcgc ctcacaatgc
      241 ggtattgcaa tggcgtgctg ctcaagcatt atcccggtag ggaattctgc ttctctggca
      301 attaatgcct gtggccaggt atcatgaacc acacctttgg cgagcatctc attaccgata
      361 tgggttaaaa cttcgctacg atcgacaaaa gaaattccgc tacgaacaaa caggttagtc
      421 atataccgtc cttattccgc acagccgtag cgataggcgc gcaaaacatc ataaatttta
      481 tccataatca gctgatgcgg tattgctgat aattccccgg actgaatgcg ttcaaattgt
      541 ttgggaagat actgactaat catgcccagt gggatgtcca cgccttgcag attcaccatc
      601 atcgtttcaa cgctattttt aatccgacta tgcggccagt aataacgaat acgatccgac
      661 aggctgtaac gaatatccag taatgaatcg ttaaaacccg tgcgataata ttttttccag
      721 tattgcggtt cgtcgagcat cacttcttca attaccgcca ggcaaccgct gcgattttcg
      781 ggggcgataa gttcctgctc aatttgcgcc agcgcaaata tcgcctcacg taaagcaaag
      841 gttaatgcgg gaccgacttt caatattgca aagtgatcgc ggactaattc ccaataagcc
      901 gtccgggtct ggtaatcggt agaatgtgct tcataaacca ttcgggtatt ttctatccat
      961 tgcgccagcg cctgcgcttc ctgcggctga taatggataa tattgctgtg atcaaattcc
     1021 acacccggct gcaccacgat ggcaatcacg cgtgttaatg cctctgtcag cccacgggca
     1081 ataaaggcct tttgatgcgt acgtaaagta ttggcggcat cttcaacatg ggtgatgtgt
     1141 actgactgaa tggcgctggc ctcaccgccc ggaaccggaa cttcggtgcc aatgacatag
     1201 ctcagttgct cacgctggca atctgtcgcc acactttccg cagcaaagca aagcacagca
     1261 gctcgttccg caaccgtttc tggtgctaac ggtatgggat cccccgcgca ggacattgac
     1321 gcatcaagat gaattttact gaagccagca cgaacatatg cctttaccag ctcgacggat
     1381 ttttccatcg ccgcatccac attttcttgc tgccagcagt ttggccccag atggtcaccg
     1441 ccgagaataa tacgctcgcg tgcaaatccg actttatcgg caatcgcaaa aacaaattcg
     1501 cgaaagtctg ccggtgtcat tccggtataa ccgccaaatt gattgacctg gtttgacgtt
     1561 gcttcaatca gcactttgcg cgtgctgttg cgatcaaatg ccagcgccgc ttcgataacc
     1621 aacggatggg cagaacagac tgaacatatg ccgatatgtt caccagcttt atgccgggca
     1681 attaacgttt tcatgttttt tccttgttaa atggcaggtg cgttatgccc tgccctcgca
     1741 gccacaatcg gcaatcactt tgctcaccac atcgcgcatt gcggatttag ccgactgcaa
     1801 ataatcccgg ggatcggtcg cttcggggtg ctcggtcagg taatttttta acgcctgcga
     1861 gaaggcattt ttcagctccg ttgcaacgtt gattttgcat atccccagtt tgatggtttg
     1921 ctgaatatcc ttagtcgata accctgacgc gccatgcagc accagcggta agttcaccca
     1981 ctggcgaatg ttctccagtc gagaaaaatc aagcgccggt gcgctggcat acataccatg
     2041 agccgtgccg atggcgaccg ccagggaatc aattccggtt gcctcggcaa attcacgcgc
     2101 ctgagcgggg ttggtataaa aagcatcggc ttcattgact tgcacatcat cttcctggcc
     2161 gccaagttgc cccagctccg cttcgacgct gacatcaaag cgatggcaaa aatccaccac
     2221 ctctttgacc cgtgaaatat tttgcgcaaa aggcaaatgc gaggcgtcaa tcatgactga
     2281 gcgcacgcca gaacgaacct tctgagcgat atcgtcaaat ttcgtgtgat ggtcgagatg
     2341 aatcgccagt ggatggtgat aatgcttcgc catcgcgctg accagcgcca acagattttc
     2401 tgtaccagca tgagtaaatg tgccaggcgt tccggcgatg atgaccggcg catgcaggtt
     2461 ggcagcggtt tctaccacca cttgcatcgt ttcgagattg tgaatattga atgccggaac
     2521 cgcgtaaccg ccgcgctgcg cgttgttcag catctgcttt gtcgatacca cgtacatttt
     2581 cacatcctgt cgattgcttt cgatttcaaa acataatgaa atcatttgtt tttaaatgtc
     2641 gagataacga tcacaaaaac gacaatatga aaattaatag aggaatgaaa ggcaaaaaaa
     2701 acggcctccc ggagggaagc cgcaacaaag tgcgcgtggt tttatgcagg atgcggcgta
     2761 aacatatgtg gcagcgctgc gtaggcatga taagacgctt cagcgtcgca tcaggcatag
     2821 gttgccggat gcggcgtaaa cgccttatcc gtcctacggg gtagtgccgt tcgtatcagg
     2881 cgatagtaat tttgctatcg agataaacgt cctgcacggc gttaatcagt ttcacgccgt
     2941 cagccatcga tttcttgaac gccttacgtc caagaatcag ccccattccg ccagcgcgtt
     3001 tgttgataac cgcagtacgc actgcatcac tgaggtcagt ttcaccgcct gcagcaccac
     3061 cggagtttat caacccggcc cggcccatat agcagttagc taactgataa cgcaccagat
     3121 caatcgggtt ttcgctggtt aacttgctgt acacgcgatc gtcggtataa ccgtaattaa
     3181 ttgctttata gccgccgtta ttttccgcca ttttttgttt gacgatatct gcacctatgg
     3241 ttgccgccag atggttcgcc tgaccagtca ggtcggcgga gacatggtaa tcaacgccat
     3301 ctttcttaaa ggcggagtta cgcaaatagg cccacagcac tgtcaccatg cccagctcgt
     3361 gcgcacgttc aaaagccgca gaaatttctt caatctggcg acgtgactct tccgagccaa
     3421 aatagatagt cgcaccaacc gccaccgcgc ccatgttgaa ggcctgctcc acgctggcat
     3481 acagcgtttg atcgtaggta ttcgggtaac ttagcgtctc gttgtgatta agttttacga
     3541 gaaatggaat gcgatgcgca tagcgccgcg ataccgacgc caacacgccg taagttgatg
     3601 ccacacagtt acagcccgct tcgatcgcca gttcaacaat gtttttcggg tcaaagtaga
     3661 gcgggttagc agcaaatgaa gctccggcag agtgctcaac gccctggtca accggcagaa
     3721 tagaaagata tcctgtgcca gccagacgcc cggtgttgta caacgtctgc atattacgta
     3781 acaccgctgg cgggcgatta ttgtcaatca tcacgcggtc tacgtagtca tgtccgggga
     3841 gataaagctg gtcagaagga atagtcatac aacggtgctg taaaaggttg tcggcgtctt
     3901 tgccaagcaa ctgcgcaata tctgtcatta ctatgctccc gtaaattccg attggatatc
     3961 ggctatggat tgtcctggcc cgccttttgc gggcaatcat aatcctggtc gttacgggcg
     4021 agtttttcca tcttttggat gttttttcag cgttttctgt tggctcgatt catcagaaaa
     4081 aatgttagcg cggtcaagtt ttcaccgcaa aggtatttaa aaggtattat taagtggtat
     4141 tgtcatcgcg taccttacat tacctgtcat gaaggaatta aaagatgaaa acaacaacaa
     4201 agctgtcgtt catgatgttt gttgaatggt ttatctgggg cgcgtggttt gtgccattgt
     4261 ggctgtggtt aagtaaaagc ggttttagtg ccggagaaat tggctggtcg tatgcctgca
     4321 cagccattgc ggcgatcctg tcgccaattc tggttggctc catcactgac cgctttttct
     4381 cggcgcagaa agtgctggcg gtattgatgt tcactggtgc ggcgctgatg tatttcgctg
     4441 cgcaacagac cacttttgcc gggttcttcc cgttactgct ggcctactcg ctaacctata
     4501 tgccgaccat tgcgctgact aacagcatcg cttttgccaa cgtgccggat gtggagcgtg
     4561 atttcccgcg cattcgtgtg atgggcacta tcggctggat tgcctctggt ctggcatgtg
     4621 gtttcttgcc gcaaatgctg gggtatgccg atatctcacc gactaacatc ccgctgctga
     4681 ttaccgccgg aagttctgct ctgctcggtg tgtttgcgtt tttcctgccc gacacgccgc
     4741 caaaaagcac cggcaaaatg gacattaaag tcatgctcgg cctggatgcg ctgatcctgc
     4801 tgcgcgataa aaacttcctc gtctttttct tctgttcatt cctgtttgcg atgccactgg
     4861 cgttctatta catctttgcc aacggttatc tgaccgaagt tggcatgaaa aacgccaccg
     4921 gctggatgac gctcggccag ttctctgaaa tattctttat gctggcattg ccgtttttca
     4981 ccaaacgctt tggtatcaaa aaggtattat tgcttggtct ggtcaccgct gcgatccgct
     5041 atggcttctt tatttacggt agtgcggatg aatatttcac ctacgcgtta ctgttcctcg
     5101 gcattttgct gcacggcgta agttacgatt tttactacgt taccgcttac atctatgtcg
     5161 ataaaaaagc ccccgtgcat atgcgtaccg ctgcgcaggg actgatcacg ctctgctgcc
     5221 agggcttcgg cagtttgctc ggctatcgtc ttggcggtgt gatgatggaa aagatgttcg
     5281 cttatcagga accggtaaac ggactgactt tcaactggtc cgggatgtgg acttttggcg
     5341 cggtgatgat tgccattatc gccgtgctgt tcatgatctt tttccgcgaa tccgacaacg
     5401 aaattacggc tatcaaggtc gatgatcgcg atattgcgtt gacacaaggg gaagttaaat
     5461 gaaaacagaa cgtattctcg gtgctcttta tgggcaggcg ttaggggatg cgatggggat
     5521 gccctccgag ctttggccac gcagccgcgt taaagcacac tttggctgga ttgaccgttt
     5581 tctgcctgga ccaaaggaga acaacgcggc ctgttatttt aaccgcgccg aattcaccga
     5641 cgatacctcg atggcgctgt gtctggcgga tgcgttactg gaacgtgaag gcaagatcga
     5701 cccgaatctg attgggcgta atattctcga ctgggcgctg cgtttcgacg cctttaacaa
     5761 aaacgtacta ggtccgacct cgaagattgc gcttaacgcc attcgcgacg gtaaacccgt
     5821 tgctgaactg gaaaacaacg gcgtgaccaa cggcgcagcg atgcgcgtct cgccattagg
     5881 ttgtttgctt ccggcgcgtg atgttgattc ctttattaat gatgtggcgc tggcgtcgag
     5941 cccgacccat aaatccgatc tggcggttgc gggcgcggta gtcatcgcat gggcgatttc
     6001 tcgtgctatt gacggagaaa gctggtcagc gattgttgat tcattgcctt caattgcgcg
     6061 acatgcacag caaaaacgca ccaccacgtt cagcgcctca ctggcagcgc ggctggagat
     6121 tgcgctgaaa attgtgcgca atgccgacgg cactgaatcc gccagcgaac agctttacca
     6181 ggtcgttggc gcaggtacca gcactattga gtccgttccg tgcgccattg cgctggttga
     6241 actggcacaa accgacccga atcgctgcgc cgtcctgtgc gctaaccttg gcggcgatac
     6301 agacaccatc ggtgctatgg cgacggcaat ctgcggcgcg ttgcatggca ttaacgctat
     6361 cgatcctgcg ttaaaggcgg agttggatgc ggtaaatcag cttgatttca accgctatgc
     6421 cacagcgctg gcgaaatatc gtcaacaacg ggaggcggca tgagcggcgc tcgattacac
     6481 acgctgctgc ctgaattaac cacgcgtcag ccggttatgg tgattggcgc ggcagtcatt
     6541 gatgtgatcg ccgacgctta tgccctcccc tggcgtgggt gcgatatcga actgaaacag
     6601 cagagcgtta acgttggcgg ctgcgcactg aatattgccg tggcgttaaa gcgcctcggc
     6661 atcgaagcgg gtaacgcctt gccgctcggt cagggcgtgt gggcggagat tattcgcaac
     6721 cggatggcaa aagagggctt aatcagcctg atcgataacg ccgaaggtga taacggctgg
     6781 tgtctggcgc tggttgagcc ggatggcgaa cgcactttta tgtcattcag cggtgtggaa
     6841 aatcagtgga atcgccagtg gctggcgcga ttaaccgttg cgcctggcag cctgctctat
     6901 ttttccggtt atcaactggc ctcgccctgc ggcgaattat tagtggaatg gctggaaaag
     6961 ctgcaagacg tgacgccgtt tatcgatttt ggcccacgta ttggcgatat cccggaggca
     7021 ttactggcgc ggatcatggc ctgtcgacct ttagtgtcgc tcaatcgtca ggaggctgag
     7081 attgccgccg aacgttttgc tttatccgca gagataacaa cacttggcaa gcaatggcag
     7141 gagaaatttg ccacgccgtt gatcattcgt ctcgataaag agggcgcatg gtatttcagc
     7201 aacgacgctt ctggctgcat tccggcattt cccacgcaag ttgtagacac cattggggcg
     7261 ggcgacagtc atgcaggtgg cgtacttgcc gggttggcct ctggtctgcc actggcggat
     7321 gccgtattac tgggcaatgc agtggcgtcg tgggttgtcg ggcatcgggg cggtgattgt
     7381 gcgccaacgc gcgaggaact actcctcgca cacaaaaacg tatagatcgc tgcgacagtg
     7441 gctaatgctg tactcaatag gccgttgctg ttggtcaagc gccacttgct tgatcaccag
     7501 caccggtatt ttgctatcta actgaatatg tgactgaaac tcggcatctg gcatccgggc
     7561 gctaacgcgg gaacgtgtac gttgtgggta aatgtgctgg ctgcggaaat agtcataaag
     7621 agaaattccg atggcatcaa catcatgaat taaatgcgcc ggaacccacg attcctcaat
     7681 cgataccgct tcttcatcga catagcgaat acgcttaagc aaaaagacat cacttcccgc
     7741 ttcgaccgcc agctgctgcg cgacttcttc gggacatttc acgacacgct tgttaaccca
     7801 tagcgtatcg ggctttttcc cgcgcaatac cacctgctga gaaaaacctc gcgcttcttt
     7861 caacgagtat tcgaagatgt tgttgatctg cgtgccgtaa ccgcgcgaac gcgtcaccac
     7921 accctcttct tccagcgcct gcatcgcctt gcgcaccgta atgcgtgaaa cgccggttaa
     7981 ctgacttaaa tcacgctcac cgggcaaaat attgccatgc tccagcaccc cgctgcgcac
     8041 ggcatttttt accgtttcgg caaacttaat ataaagcggc gtgttatccg ccgctaaaat
     8101 acgctcgttc agttgggcaa tcagctgggt atgcgcttgt tccatttgtc tttttcctgc
     8161 cagggagtct ggtgggagta tatcactgct tcttatttaa ggcgtttctt taatcccatc
     8221 aacgaatgcc tgcaactcct cctctgtgcc attaaacaca ttaaaatcca ccgaaccatt
     8281 aatgccatct acctgcccac ggtcggaatg ctgccagaaa cgccaggcca tgccgtcatt
     8341 gtccgggcga cgttgatagt agtgagccac ccaccacgga tattcattga aatagcccgc
     8401 cagattggtg tgataaaaaa cggctcctga gtaaataacc ggcttttttc ccgtactttt
     8461 ttcgaccatt tttagccact gacttacccg cttgcgtaat tcttttgccg ataattttcc
     8521 ccgttcttct acgtccagca cggcagggaa atcgccttgt gagaaatcca ccgtttgcag
     8581 aaataatcgc gcctgaacag gcgctgctac cgacggggag aaataatgat acgccccgcg
     8641 cagcaggcca ttttcgcggc ttagttgcca gttacgcgaa aaataggggt ccaccagctt
     8701 ttcgccttcc gtcgccttga taaaggcaaa ttgtaagcgg ataccgttat cgcgcatttt
     8761 tgccacccgc tgccagtcga tccgctcctg ccagcgcgaa acatctattc cgtgaatggt
     8821 gtaactggca ggaatatgaa tagcgaaaga tttaaccggg cgataaccgt agaagtggat
     8881 gtagtctgta atctgaactg ctgtcgaata gaaaaaattc accgtctgac gcggataaat
     8941 tgcaacgatg gaaattagcc caagagcgca taatagagcg gtgagttttt ttcgactggt
     9001 aattcttagc tgcattggtt tcatccctga atgtcagtgc caaaggctga caataaccaa
     9061 agcagctata gtacggtgct caccaccagg cgtggaagtg gtgaaccgga ccaataccgt
     9121 gaccaacttc cagcgtgtcg gcctgggcta acgccgatga aagccagctt tttgcctcct
     9181 gtacggtgtc agcccagttt gtatggcgcg ggcgtagtgc agccaacgcc gcagagagtg
     9241 tacaaccagt gccgtgggtg tttttggtca taatgcgcgg tgcggtaaac cgttgttcac
     9301 cctcgcgggt aaacagccag tccgggcttt gctcatcatc cagatggcca cctttcatta
     9361 gcactgcgcc acagcccatc gccaacagcg ctcgcccttg ttccagcatt tcctgttcgg
     9421 tgcgcgcgtg tggcgcgtcg agcaaggcag cagcttcggg caagtttggc gttattaatg
     9481 aaacctgtgg caataatcga ctgcgtagcg tggcaactgc cgaaggtgaa agcagtgggt
     9541 cgccactttt tgccagcata acggtgtcga gtaccacgtt ttggatctga taacgttgca
     9601 accgttctgc caccgcttca acaatatcgg tttccgccaa cataccgatt ttagtggtat
     9661 cgattcgcac atcgctgaac accgaatcga gctgggcggc gacaaaatca ggctcaatgc
     9721 gatacaccga ctgtacgcca cgggtatttt gcgccaccag tgcagtaata actgagcaac
     9781 cataagcgcc aagtgccgag aaggttttaa gatcggcctg aatccccgca ccaccactcg
     9841 gatcagtacc ggcaatcgtc agagcgttaa ttcgtttcat gcctgcacct cctgcgtcaa
     9901 ttgccagagc gcatcaagga aatgtggaac aaaactgcct ggcccctcgc ttctggcgac
     9961 tgcgcgttct ccggcttgtt tcatccagtg acaggcagat gcgacatttt ccagcatatc
    10021 gcctggtaac gcacaacagg cagcgacaac cgccgataat gcacagccag ttcctaccac
    10081 tttggtcatt aacggatcgc caccgtgaat gccaacggcg cgatggccat cggtaacata
    10141 atccacctcg ccagtgacca cgacgattgc gccagtttcc cgtgccagtg tttgtgcagc
    10201 gggtatcgcg ttaactgcgg cgtcagtggt atccactccc cgtccaccat tagcaacgcc
    10261 agctaatgcc atgatttccg aagcattacc acgtatcgct gccggtttaa aagataaaag
    10321 ttcatgacaa aaatggcggc gataatcgag cgcaccaacc gctactggat caagcgtcca
    10381 gggtgtttga gagctttttg cttgctcaac ggcagcacgc atcgcctgtg cgcgtggctg
    10441 cgtcagtgtg ccaacgttaa tcaacaaggc actggcgata gccgcaaact gactggcctc
    10501 ttcggtttcg ataaccatcg ctggcgatgc accgagcgcc agcaaggtat tggcagtaaa
    10561 ggtttgcacc acatcgttgg tcatgcagtg cacaagaggg gaatgttggt gaaaaaggtg
    10621 taacgtgtgc gcagattgcg cggaactcag caggtcgact tgcatagttt gctcctgcca
    10681 taacgtgaag aagcaatgac ctggtggtcc gtgacttccc tacgctggca ttatccagat
    10741 caggtgatac gggtatttct cagccttcac gcagaagggc accccgagtc gtttggttga
    10801 atctctatat tttgtgccag cttaggggaa gaggcttgca gatgcaagct cgcctccatt
    10861 cattacgtct tcactgtgta ccccatatca gtaatgaaaa agaaacaatt cacacccatc
    10921 attcaggtgg taaaaactgt agactatctt aattagctaa tttatattat ttatctaaat
    10981 ttgataggtg agataaaaat gacagaaacc cagatctacg agaacattaa gcaagccatt
    11041 agcagcgcac cacgaaatag ccaaacgatg gaaatgcacc tccagatgat caaatacgct
    11101 gaccatctga aaaacgtcac ggcaaaagag ttttgcgaag gtgtaggact taaagccagc
    11161 tttgctacag agttcagcaa aatgagaaat cttaccgagc gactcaaagc cgcc
//
LOCUS       DBFIFM010000148        11192 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.148, whole genome shotgun sequence.
ACCESSION   DBFIFM010000148 DBFIFM010000000
VERSION     DBFIFM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.148"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..879
                     /locus_tag="N7C74_003446"
     CDS             <1..879
                     /locus_tag="N7C74_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HGU2433252.1"
                     /translation="EALWEHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPGRAGALL
                     TTLLRHISTINGFDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYPTEGGMQ
                     DIIDCIRAELGVGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQGTMFGDG
                     GMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPRKRLKYA
                     GDGGNSGGLLSFTSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVLQKSLDI
                     TEEADLALRNAMARIK"
     gene            1015..1698
                     /locus_tag="N7C74_003447"
     CDS             1015..1698
                     /locus_tag="N7C74_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433253.1"
                     /translation="MKLPVKLLMSLISLVRVIARAGEYKNYSRDEIKYWRYTSYKGGK
                     LPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCNPEA
                     WAEQPGACPSTYELYEGDATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNING
                     TVWFQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYL
                     VKNYVAAVN"
     gene            1714..2124
                     /locus_tag="N7C74_003448"
     CDS             1714..2124
                     /locus_tag="N7C74_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433254.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVGVAQDKLKI"
     gene            2345..3166
                     /locus_tag="N7C74_003449"
     CDS             2345..3166
                     /locus_tag="N7C74_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU2433255.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHTSRSER
                     YAYIPTITVLENLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPQPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            3248..3727
                     /locus_tag="N7C74_003450"
     CDS             3248..3727
                     /locus_tag="N7C74_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU2433256.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPEGSSIMRIID"
     gene            3742..4218
                     /gene="yeeS"
                     /locus_tag="N7C74_003451"
     CDS             3742..4218
                     /gene="yeeS"
                     /locus_tag="N7C74_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HGU2433257.1"
                     /translation="MQQISFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            4281..4502
                     /gene="yeeT"
                     /locus_tag="N7C74_003452"
     CDS             4281..4502
                     /gene="yeeT"
                     /locus_tag="N7C74_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGU2433258.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            4576..4944
                     /gene="cbeA"
                     /locus_tag="N7C74_003453"
     CDS             4576..4944
                     /gene="cbeA"
                     /locus_tag="N7C74_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HGU2433259.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHPDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            5033..5406
                     /gene="cbtA"
                     /locus_tag="N7C74_003454"
                     /pseudo
     CDS             5033..5406
                     /gene="cbtA"
                     /locus_tag="N7C74_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
     gene            5403..5597
                     /gene="yeeW"
                     /locus_tag="N7C74_003455"
     CDS             5403..5597
                     /gene="yeeW"
                     /locus_tag="N7C74_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGU2433260.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            5643..5723
                     /locus_tag="N7C74_003456"
                     /pseudo
     CDS             5643..5723
                     /locus_tag="N7C74_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6012..6158)
                     /locus_tag="N7C74_003457"
                     /pseudo
     CDS             complement(6012..6158)
                     /locus_tag="N7C74_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            6212..6394
                     /locus_tag="N7C74_003458"
     CDS             6212..6394
                     /locus_tag="N7C74_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HGU2433261.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKCWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCR"
     gene            complement(6495..6824)
                     /gene="tmaR"
                     /locus_tag="N7C74_003459"
     CDS             complement(6495..6824)
                     /gene="tmaR"
                     /locus_tag="N7C74_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HGU2433262.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(6996..8054)
                     /gene="yeeA"
                     /locus_tag="N7C74_003460"
     CDS             complement(6996..8054)
                     /gene="yeeA"
                     /locus_tag="N7C74_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HGU2433263.1"
                     /translation="MRANKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     GTWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSSLICRALRK"
     gene            complement(8253..8726)
                     /gene="sbmC"
                     /locus_tag="N7C74_003461"
     CDS             complement(8253..8726)
                     /gene="sbmC"
                     /locus_tag="N7C74_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HGU2433264.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(8845..10011)
                     /gene="dacD"
                     /locus_tag="N7C74_003462"
     CDS             complement(8845..10011)
                     /gene="dacD"
                     /locus_tag="N7C74_003462"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HGU2433265.1"
                     /translation="MKRRLIIAASLFIFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
BASE COUNT         2917 a         2830 c         2661 g         2784 t
ORIGIN      
        1 gaggcgctct gggagcatct ggggaataat caggtgctgt cggtaaacag attggtttac
       61 ttttcattgc tgaaaaaatt gttccaggca atgaacctct gccagatccc cggacgtgca
      121 ggagcactgc ttacgacgct tcttcgccat atatcgacaa tcaacgggtt tgacaatctg
      181 atggctcagc cgttgttgtc agatgagccc ctgacagcgc tgatggatca ttatcttgat
      241 actgatgctc tggcagacgg gctaccgctg tatgtgtcgc tgtaccccac agaagggggc
      301 atgcaggata ttattgactg cattcgtgct gaactgggtg tcggaaccac gaaaaacgcc
      361 gtttttcagc atatccagag cctgccccgc ggacagcaga aagaggctct gcttgcgtca
      421 gccgcgctgc ccctgctgtt ccgtccccgt gaggttcagg ggacaatgtt cggtgatggt
      481 ggtatgggag gatggcgaaa tatgcaggga aatacccctg tgacgcctct ggtcgatgcc
      541 ggatgcaata tggtgattgt gacgcatctg agtgacggtt ctttatggga tcgccaggct
      601 tttccggaca ccacaatcct tgagatccgt ccccggaaaa ggctgaaata tgcaggtgat
      661 ggtggcaaca gcggcggtct gctcagtttt acatcggcac ataccgacgc ctggcgtcag
      721 cagggctatg aagacacgat gctggcgatg gagcatatcc ggaaaccgct ggcagcacgt
      781 caggcactga cccggtcaga ggcggtattg cagaaaagcc tggatataac ggaagaggca
      841 gatttggcac tgagaaacgc gatggcccgg attaaataaa gacgccccaa agaaacgcca
      901 ccgcctgtgg tggtgttttt cctgtctggt gttattgaaa atattttcag atatatatta
      961 cctgagagaa agttttaccc ctcacctgtc aattcactca ctggaaattt aattatgaaa
     1021 ttacctgtaa agttattaat gagccttata tctctggtcc gcgttattgc acgtgccggg
     1081 gaatataaaa attacagccg ggatgaaata aaatactggc gatatacatc atacaagggg
     1141 gggaaacttc cggaaggttt cactgatgag aaattttcca gcgccattta caacggaaga
     1201 atatttacaa tgaaacgttt acataccctt atgttatttc tggcggttct gttcacgggc
     1261 tttaacttta acgcagaagc ggctaccgta aaacaagctc ttagttgtaa cccggaagcc
     1321 tgggctgaac aacctggagc gtgtccatca acgtacgagt tgtacgaagg tgacgctacc
     1381 tacaaagccg ctatcgacaa agcattaaaa ccggtcggac tgagcggcat gttcggtaaa
     1441 ggcggctata tggatggccc tggtggaggt atcacgccag taaacattaa cggtacagtc
     1501 tggttccagg gcgacggttg caaagccaat acctgcggct gggactttat cgtaacactc
     1561 tataacccaa aaacccatga agttgttggc taccgttact ttggtttaga tgacccggcc
     1621 tacctggttt ggttcggtga aattggcgtg catgaattcg cgtatctggt gaaaaactac
     1681 gtagctgcgg ttaactaatt taaggagtct atcatgaaaa cttttatcaa aactttactc
     1741 gttgctgtaa ctattctgtt ctctgtcttc gctactgcga aacaagtaaa actgccaaat
     1801 aacatcaaat acgttaatac tacagaagcg ttttcctgta ccgaaattga cggtatgaat
     1861 tgccagacaa agaatcagtt caactataaa gataacagct atgttttcgt gcttgaacgt
     1921 ggtggtgcct ggtgctacga ctacactgtc tcggtagtta acctgaaaac cgggaaagca
     1981 caaatgattg aatacgggga caatcaactg tgctcaggta gcaacaaacc gttcttcgaa
     2041 atcaaaaatg gcgtaccgac ggtaggagtc atcgacacat ccggaaaacc tgtcggtgta
     2101 gcccaggaca aacttaaaat ctaaggagta aaccatgcgt ttctctgcac attattaata
     2161 tcaaaggtaa atacagcccc agccattgtt accggaaagc cgcttaatgc gtggctcgcc
     2221 agtgaaaggc ctctcagaca ataaacctta ttcatttcac ccgtcaggcc gcgtcttctc
     2281 cgggagacgc ggcttttttc atttattgca ccagtaaatc ttaaccaccg ataaggagca
     2341 aagtatgcga ttagccagtc gttttggtta tgctgcaaac cagatacgcc gtgaccgtcc
     2401 gctgacacat gaagaactga tacgccatgt acccagtatt tttggggaag accggcacac
     2461 ctcccgcagt gaacggtatg cgtacattcc caccattacc gtcctggaaa atctgcagcg
     2521 ggaaggcttt cagccgttct tcgcctgcca gacccgtgtg cgcgacccgg gccgccgggg
     2581 atacacaaaa cacatgctgc gtctgcggcg ggccggagag ataaacggag aacatgtccc
     2641 tgaaattatt ctgctcaact ctcatgacgg tacctccagc taccagatgc tgccgggtta
     2701 cttcaggttc gtctgccaga acgggtgcgt ctgtggccag tctctggggg aagtgcgtgt
     2761 tccacaccgg ggaaatgtag tggagaaagt tatcgaaggg gcttacgagg tggtgggcgt
     2821 gtttgaccgg atagaggaga agcgtgatgc catgcagtcg ctggtcctgc cgcaaccggc
     2881 acgccaggcg ctggcacagg cggcactgac ttaccgttat ggtgacgaac atcagcccgt
     2941 caccaccgcc gacattctga cgccgcgacg ccgggaggat tacggtaagg acctgtggag
     3001 cgcatatcag accatccagg agaatatgct gaaaggcggg atttccggtc gcagtgcaaa
     3061 aggaaaacgt atccataccc gggccattca cagcattgat accgacatta agctcaatcg
     3121 cgcattgtgg gtgatggcag aaacgatgct ggagagcctg cgctgatgcc gtttccctga
     3181 gtgagggaat cattcagctt tcgttcccga aaaacacaca tttatttgtt atttaaggag
     3241 ttatctcatg aaaaccgttt ctcagaatac ccccacaatt tattcagcta caacaccaga
     3301 gaataatccg cctcagttgg ttgccagcct cgtccctgat gaacagcgca tcagcttctg
     3361 gccgcagcat tttggcctca ttccacagtg ggtcaccctg gagccccgtg tcttcggctg
     3421 gatggaccgt ctgtgcgaag actactgcgg tggtatctgg aatctgtaca ccctgaacaa
     3481 cggcggggca tttatggccc ccgaaccgga tgatgatgat gacgaaacat gggtactgtt
     3541 caatgccatg aacggtaacc gcgctgaaat gagcccggaa gccgccggta ttgccgcctg
     3601 tctgatgacg tacagccatc atgcctgtcg tacggagaat tatgccatga cggtccatta
     3661 ttaccggttg cgggattacg ccctgcagca tccggaaggc agctccatta tgcgcatcat
     3721 tgactgaaag gggccggaat aatgcaacag atttcctttc tgcccggaga aatgacgccc
     3781 ggcgagcgca gcctcattca acgggccctg aaaaccctgg accgccatct tcatgaaccc
     3841 ggtgtggcct tcacctccac ccgtgcagca cgggaatggc tgattctgaa catggcggga
     3901 ctggagcgtg aagagttccg ggtgctgtat ctgaacaacc agaatcagct gattgccggt
     3961 gaaaccctct tcaccggcac catcaaccgc acggaagtcc atccccggga agtgattaaa
     4021 cgcgccctgt accacaatgc cgctgccgtg gtactggcac acaatcaccc gtccggtgaa
     4081 gtcacaccca gtaaggcaga ccggcttatc accgaacgtc tggtacaggc actgggcctg
     4141 gtggatatcc gggtgccgga ccatctgata gtcggtggca accaggtttt ctcctttgcc
     4201 gaacatggtc tgctttaacc cgtcacaacc acatcacacc tgttttcact tttatcttct
     4261 gtcttcagag gtatcccatt atgaaaatta tcacccgtgg tgaagccatg cgtattcacc
     4321 aacaacatcc gacatcccgt ctttttccgt tctgtaccgg taaataccgc tggcacggca
     4381 gtgctgaagc gtataccggt cgtgaagtgc aggatattcc cggtgtgctg gccgtgtttg
     4441 ctgaacgccg taaggacagt tttggtccgt atgtccggct gatgagcgtc accctgaact
     4501 gagtgggaat tctgatgagc agaattatcg ccactaccgg accattctta gccgattttc
     4561 tgtaaggatt ttatcgtgtc agacacactc cccgggacaa cacttcccga cgacaatcac
     4621 gaccgcccct ggtgggggct gccctgcacc gtgacgccct gtttcggggc acgtctggtg
     4681 caggagggta accggttgca ttaccttgcc gaccgcgccg gtatcagagg cctgttcagc
     4741 gatgcagatg cgtaccaccc ggaccaggcc tttccgctgc tgatgaaaca actggaactc
     4801 atgctcacca gcggtgaact gaatccccgc catcagcata ccgtcacgct gtatgcaaaa
     4861 gggctgacct gcaaagccga taccctcagc agttgtggtt acgtttatct ggctgtttat
     4921 ccgacgcccg aaatgaaaaa ttaactctcc agaatagcct tctgctacgg cctggtgttt
     4981 tcaccacgcc acttttccat ttttatatct gcatatcagg aaaatcttca gtatgaaaac
     5041 attacctgta ttacccgggc aggcggccag ttctcgcccg tctcctgttg aaatctggca
     5101 gatactgctg tcccgactgc tggaccagca ctatggcctc acactgaatg acacaccttt
     5161 tgccgatgaa cgtgtgattg agcagcatat tgaggcaggc atttcactgt gtgatgcggt
     5221 gaactttctc gtggaaaaat acgcgctggt gctaccgacc agccgggatt cagcgcctgt
     5281 acccgctctc agttaataaa cagcatcgat atcctccggg ctcgcagggc gaccggcctg
     5341 atgacccgcg acaattacag aacggtaaat aacattaccc tgggtaagta tccggaggcg
     5401 aaatgatgac actggaagcc gacagcgtta acgtacaggc gctggatatg gggcacattg
     5461 tcgttgacat tgatggtgtt aatatcactg aactgattaa taaggccgct gaaaacggtt
     5521 attcactccg cgtggtggat gaccgtgact ctaccgaaac accggcaact tatgccagcc
     5581 ctcaccagtt gctgtaagac aatgcagtga tgcagggtat agtgccatta accacagaaa
     5641 cagcactgtt gtcctgtatt aagcaggcaa gagaagatgc ccttaccctg cgccatctgc
     5701 agcttctgca ccagaaccgg tgaatctcac tcaacaggct tcatttcccc tgacgccagc
     5761 ctgaatacag ctggcgtttt catttataaa cagaaaggaa aaccgataat aatggaatgg
     5821 acgccccgac catgaagcaa cggtaggata ttactgataa accttccagt ggaggtgcgt
     5881 tatggccgtt ttaacgatta gcattgacct gacaaaaaat gaattccaaa tccacggtct
     5941 gggtaggaac agaaaaatct aagctcagaa aacggattaa agtaagcgtc aacggagcac
     6001 cgtattgacg cttatttatt ggtgagaact acgttccatg gcaggagttc gtcaacacgg
     6061 ttggagggcc attccggcag tatgctcaga atatggcgca gatacgcttc cggatcgata
     6121 ccgttcagac ggcaggtgcc tgcttttccg gcaacaagca ggaatctcag cgctgaagca
     6181 ggaattagag gtaaaaacgc cctaccgggc catgaatcac ccggtcattg gagtagtaac
     6241 caaagcagat ttagccagca tggaacaaat atctttggtg aaatgttggt tacgggaagc
     6301 tggagcgcac aacgtgttag taaccagtgc ggttaataac aatggagtta cagaactttt
     6361 tgccttgctg catacagaag aaggctgccg ctaatttcga taggttctca gatgagaatg
     6421 atgacggtga tacacagata agaatcaatg agcaatgagg gttgccgggc aaccctcatt
     6481 gaataaaaca ggaattactt cgcttcgccg tttttcattt cgcccatagc tttcagcttt
     6541 ttggagatat cgcggcgttc tttggagagc tcggcatttt tgatgatgta atcgtcaacg
     6601 cgatcttcat agtcaccttt catgctggcg atgatgccct ggattgcttc aacgctcatc
     6661 cctggcttga tgtaatcgct caggttgtcc agcaacagga cgcgcttctg gttgtcacgg
     6721 atctttttct caacgtcctg aatttcacgt tgcagtttgt tcttacgacg gaacagacga
     6781 acaaattcca gtacgtcctg gaatgaaggc ttggtagttt ccatttttat acccctgata
     6841 atgtgagagt cggattcgtt taagcaaccg ctattcgtta gggccaacat tactcatagc
     6901 tgccgcagat gacaatgctt ttatccctta tcactatcat accctttatc cttgctgaat
     6961 cgaagcagca gcaagatgat tctgaagttc aggaattatt tgcgcaaggc ccggcaaatc
     7021 agactcgata gcaactcaag ctgatgtgcc gtttccatgt tcagccacac ataaccatag
     7081 attggtgttt ccacaacctt caggtcatgg tggttattca gcaactgacg cagctcttcc
     7141 acagcatcgt tcaatttttc cgtattggca aaaaccggct gcggattacc ttcgtacagc
     7201 gcatgaacaa ggctcagcag tatttgctgc atcatgtgct gggtatcacg aagtttttgc
     7261 gcgttcaata acacgaaatg gctggggcgc gtggcccagt atgcattgat ttgcaactcc
     7321 agcatacaaa ccagattgcg gttaatggtc tggatacctt catatatcga ttttggaata
     7381 cgagtttctt tgctggcggg cgcaatcagc ccacgcattt tcacggcatc ggtcaggagt
     7441 ttttgtagat ggctttccag acgtgggcgt tcgagtaagt tcggtgagaa tgcagattga
     7501 tagacccgat tatactcggt cagacttttc gccagttgaa tgcgccagtg gatgaacgcc
     7561 cgttgtggcc agataccggt aaacaacatt gccagtaaag agccgaggat cacatcgccg
     7621 cttcgccata acgccgtatc aatttcacct gtcggggaac ccacaacaat tgccagcgtc
     7681 accccaatca ataatccttg atacggtttc ttgcccagcg ccagccaacc gcaaagaaac
     7741 atcgccgccg cgcaccagac taacatcagc ggtaacgaaa ttaactccag ttgcagagcg
     7801 ataagaccta aaatcgaccc caacaccgta ccgccaatac gttcgaaggc acgagggaca
     7861 acgttccccc agaacgagat tggccccata atcaccacca tggtgaccag cggccaggtg
     7921 ccttccggga tagtaaacag gcggataatg agaaaagtga gcaggaatgc cagcgcgacc
     7981 cgagtaccat gcacaatgcg gtaatggcgg tatacccgga tttcaaacgg gcttaatgac
     8041 ttattggcac gcacgcagaa actccgttaa tgaagattaa aagcgctcat aacacatctg
     8101 tcggtcagaa ggcgcgcctc tgaattatcc gacggacaaa ccgggaacag tgtaatttcc
     8161 cggcaggagc gaaaaaattg aaaggcgcat cttactcttt tcgctttcaa aaaaagagtg
     8221 gtcttcgcgt caacacaccg ccctgagatg aattagtgat gttttggctg caccgcaaca
     8281 tacatttcga tatcccagta cccatcttcc gcgccattgt tcaagtaaac ctcgaagcag
     8341 ggctttggta acatttcata agcactgtcc tgcaagaggc tattaaagaa ctgataccag
     8401 ggtttagcaa aatcatcacc gactacacga gccaccgcca ccgcatattg accacctgaa
     8461 atttctgtca gaatgacgcc ctcactgttt tcggggagcg taaagttatt cggtaccgtc
     8521 acgacggtgt cgcagcgtaa tttttcggcg ggtgtttcat ccggattgtc gtaatagaca
     8581 gcaacccact ccttcggcac aatatttttg ctatctaccc acatcatcaa ctgctcaaaa
     8641 cctttcttta ccgtctgttc ccacgggcca acgagatgga aacctgcaac ggtacgttta
     8701 tcttcctgct taatctcgta gttcatgacg cctccattat tactgttttt atatacagta
     8761 tagttgcaat attaaaacca caaggaatga gtgttgatta tgcgagcaga ctcgcactcc
     8821 tgccagtctg ctgcaaaaga aaggtcaggc cttatggtgg aaataatcac tcaggcgaga
     8881 aaacatgctg ccttccccga cagattccag ggtaaccagc ggccagtgcg ccacctgttt
     8941 atcacggtcg taaagttcaa tttcccctac ccgctgatgg gcgctaattg gcgcggtgag
     9001 ctctttacca tcaagggtat atttggcttt gatatgtgga atttcggctt tcggtagcac
     9061 catccagaac tcttgttccg ttcccagggc gatattttct ttatcgccat accagatgcg
     9121 ttccgtaccg acctttttcc cacggtgcaa aatttgcacc gtagtaaagt tttgttgccc
     9181 ccaacgcagt aattttcttg cctcttcctc acgacctttt gcactgtcag cccccattac
     9241 cactgcaatg agacgacgct gcccatctac agccgaagca atgagattaa acccggcacc
     9301 agaagtatga cccgttttca ggccgtcaac attcatggtt ttatcccaca ataacccgtt
     9361 acggttttgc tgggtgatac cgttccaggt gagacttttc tcactgtaca tatgataaaa
     9421 ctcgggctcg ccgtggatga tagcgcgaga aagcacagct aaatcataag ccgagctatg
     9481 ctgacctggt gcatccagac catgcactgt ttcaaaatgc gtatccttga gatgcagctt
     9541 ctcggcatag ttgttcatca tttcaacaaa ctgccgttgc ccaccggcaa tatagtcagc
     9601 cagtgcaaca caagcgtcat ttccggaatc cacaattaaa ccacggctta aatcacgtac
     9661 cgatacgcga tcgccctctt tcaaaaacat cagtgaagaa ccgacaaaca ctggattatc
     9721 tttcgcccac gcatcgcgcc ccacggtgac aatatcgtct ggcgtaatgc gatgactatc
     9781 gatagcgcga tccacgacat agcccgtcat cagctttgtc aggctggcgg gattgcgctg
     9841 ttgatgctca ttacccgcgg tgaggatctg accggtggtg taatccatca atacccagga
     9901 gccggcatga atctctggag gctgaggtga aaaaggaatg ttttccgccg caaaaccaga
     9961 cgataagtta aaaatgaaca aagaagcagc aataataaga cggcgtttca acagcaaacc
    10021 ctcaggagtt tcaaatagct gttcttttta cggaaatact tatgaactgg ctggaataaa
    10081 gtgcaagaaa atgtgactac cctctcattt ttatctgaca tgatctgttg ccactcgctg
    10141 tcaaattgtc gcgctaaagc tgattagcac ggtgatattt gatactctgg cagacagcag
    10201 aaacaacgga tttaacctaa tgatgaatga cggtaagcaa caatctacct ttttgtttca
    10261 cgattacgcg caacacccct gagaaccgcc atgaacaagt gtttcatgat ttgtgatttt
    10321 agtttggtac gcaatttggt acacaacaca attttcactt cagtgggtag tcatcaaact
    10381 caccataacg agcatcgttg atgatgtatg ttataaccac aaacatcaca tctggactag
    10441 ttccgttttc atgagcgggt atttgtttac gcctcccttt ctgcaaatcc taaagatgag
    10501 attttggggt tttgggatat cttttatcct cagttctcta tccattatgc aaaccagtaa
    10561 tgaaccatca ccgtgtgata gtgaggcgtc tgccacgata agcgcagaga aatgatgcaa
    10621 tggtgggcgg actggcttga tgaaaaggta gagtgagcga ccttaaacta tcgaatagaa
    10681 caaagtcttg caatccagtg caaagctttg tgcgcctcag ttttgtctaa gcgttctact
    10741 gaaaacatag taaaatcggt aacggctgga aatcattcaa tactcgcact atcgaaagtt
    10801 cgccagccag ccgtggcacg ttcttgcata cgacgtgccg cggatcctat tattctagta
    10861 tgaaatccct aaccgatttc aaattctcca gactgctcag attctctttt atcccacgct
    10921 agcgctgata aatctcgtca ttgcaatcga cctgcgctat cgctgccgca gttcttccag
    10981 tttctgcatc gataactcca tcttctgagc aataaacctc acctatgcaa tattttatgt
    11041 caattatttc aattgcatta atatcttgaa tggattacat agagttaatg tatccagtac
    11101 tccctattct cttgcatata atacatgttg caacttacat ctcagcgcta tgaaaaaaca
    11161 ccacctctct ctttatgaaa ttctggattt ac
//
LOCUS       DBFIFM010000149        11186 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.149, whole genome shotgun sequence.
ACCESSION   DBFIFM010000149 DBFIFM010000000
VERSION     DBFIFM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.149"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..183
                     /locus_tag="N7C74_003463"
     CDS             <1..183
                     /locus_tag="N7C74_003463"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433266.1"
                     /translation="NGFSGEKRILYGHSAHHFFFSSSTIHVSLPRLITDYEVQKYLSF
                     QLPVIRYRERFWQYME"
     gene            190..2613
                     /gene="pgaA"
                     /locus_tag="N7C74_003464"
     CDS             190..2613
                     /gene="pgaA"
                     /locus_tag="N7C74_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HGU2433267.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2622..4640
                     /gene="pgaB"
                     /locus_tag="N7C74_003465"
     CDS             2622..4640
                     /gene="pgaB"
                     /locus_tag="N7C74_003465"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HGU2433268.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRKSLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFIWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            4633..5958
                     /gene="pgaC"
                     /locus_tag="N7C74_003466"
     CDS             4633..5958
                     /gene="pgaC"
                     /locus_tag="N7C74_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HGU2433269.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERRWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYSLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            5960..6373
                     /gene="pgaD"
                     /locus_tag="N7C74_003467"
     CDS             5960..6373
                     /gene="pgaD"
                     /locus_tag="N7C74_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HGU2433270.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6423..7211)
                     /gene="phoH"
                     /locus_tag="N7C74_003468"
     CDS             complement(6423..7211)
                     /gene="phoH"
                     /locus_tag="N7C74_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HGU2433271.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7831..9102)
                     /gene="efeB"
                     /locus_tag="N7C74_003469"
     CDS             complement(7831..9102)
                     /gene="efeB"
                     /locus_tag="N7C74_003469"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HGU2433272.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPVARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKGIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(9108..10235)
                     /gene="efeO"
                     /locus_tag="N7C74_003470"
     CDS             complement(9108..10235)
                     /gene="efeO"
                     /locus_tag="N7C74_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HGU2433273.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10293..11123)
                     /gene="efeU"
                     /locus_tag="N7C74_003471"
     CDS             complement(10293..11123)
                     /gene="efeU"
                     /locus_tag="N7C74_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HGU2433274.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         2923 a         2778 c         2632 g         2853 t
ORIGIN      
        1 aatggatttt cgggcgagaa aaggatttta tatggacact ctgctcatca tttcttcttc
       61 tcatcatcaa caattcacgt ctctcttccg cgtttaataa cggattatga ggtgcaaaaa
      121 tatctttctt ttcagttacc tgtaattaga tacagagaga gattttggca atacatggag
      181 taatacagga tgtattcaag tagcagaaaa aggtgcccga aaaccaaatg ggctttgaaa
      241 cttcttactg ccgcattttt agcagcgagt cccgcggcga agagtgctgt taataacgcc
      301 tatgatgcat tgattattga agctcgcaag ggtaatactc agccagcttt gtcatggttt
      361 gcactaaaat cagcactcag caataaccaa attgctgact ggttacagat tgccttatgg
      421 gccgggcaag ataaacaggt tattaccgtt tacaaccgct accgtcatca gcaattacca
      481 gcgcgtggtt atgcagctgt cgccgtcgct tatcgtaacc tgcaacaatg gcaaaactcg
      541 cttacactgt ggcaaaaggc gctctctctg gagccgcaaa ataaggatta tcaacgggga
      601 caaattttaa ccctggcaga tgctggtcac tatgatactg cgctggttaa acttaagcag
      661 cttaactctg gagcaccgga caaagccaat ttactcgcag aagcctatat ctataaactg
      721 gcggggcgtc atcaggatga attacgggcg atgacagagt cattacctga aaatgcatct
      781 acgcaacaat atcccacaga atacgtgcag gcattacgta ataatcaact tgctgccgcg
      841 attgacgatg ccaatttaac gccagatatt cgcgctgata ttcatgccga actggtcaga
      901 ctgtcgttta tgcctacgcg cagtgaaagt gaacgttatg ccattgccga tcgcgccctc
      961 gcccaatacg ctgcattaga aattctgtgg cacgataacc cagaccgtac tgcccagtac
     1021 cagcgtattc aggttgatca tcttggcgcg ttattaactc gcgatcgtta taaagacgtt
     1081 atttctcact atcagcgatt aaaaaagacg gggcaaatta ttccgccctg ggggcaatat
     1141 tgggttgcat cggcttatct caaagatcat cagccgaaaa aagcacagtc aataatgacc
     1201 gagctctttt atcacaagga gaccattgcc ccggatttat ccgatgaaga acttgcggat
     1261 ctcttttaca gccacctgga gagtgaaaat tatccgggcg cgctaactgt cacccaacat
     1321 accattaata cttcgccgcc tttccttcgg ttaatgggca cgcctacgag catcccgaat
     1381 gatacctggt tacaggggca ttcgtttctc tcaaccgtag caaaatatag taatgatctt
     1441 cctcaggctg aaatgacagc cagagagctt gcttataacg caccaggaaa tcagggactg
     1501 cgcattgatt acgcgagtgt gttacaagcc cgcggttggc ctcgtgcagc agaaaatgaa
     1561 ttaaaaaaag cagaagtgat cgagccacgt aatattaatc tggaggttga acaagcctgg
     1621 acagcattaa cgttacaaga atggcagcag gcagctgtct taacgcacga tgttgtcgaa
     1681 cgtgaaccgc aagatcccgg cgttgtacga ttaaaacgtg cggttgatgt acataatctt
     1741 gcagagcttc gtatcgctgg ctcaacagga attgatgccg aaggcccgga tagtggtaaa
     1801 catgatgtcg acttaaccac catcgtttat tcaccaccgc tgaaggataa ctggcgcggt
     1861 tttgctggat tcggttatgc cgatggacaa tttagcgaag gaaaagggat tgttcgcgac
     1921 tggcttgcgg gtgttgagtg gcggtcacgt aatatctggc tcgaggcaga gtacgctgaa
     1981 cgcgttttca atcatgagca taaacccggc gcgcgcctgt ctggctggta tgattttaat
     2041 gataactggc gtattggttc gcaactggaa cgcctctctc accgcgttcc attacgggca
     2101 atgaaaaatg gtgttacagg caacagtgct caggcttatg ttcgctggta tcaaaatgag
     2161 cggcgtaagt acggtgtctc ctgggctttc actgattttt ccgacagtaa ccagcgtcat
     2221 gaagtctcac ttgagggtca ggaacgcatc tggtcttcac catatttgat tgtcgatttc
     2281 ctacccagtc tgtattacga acaaaataca gaacacgata ccccatacta caaccctata
     2341 aaaacgttcg atattgttcc ggcatttgag gcaagccatt tgttatggcg aagctatgaa
     2401 aatagctggg agcaaatatt cagcgcaggt gttggtgcct cctggcaaaa acattatggc
     2461 acggatgtcg tcacccaact cggctacggg caacgcatta gctggaatga cgtgattgat
     2521 gctggcgcaa cgctacgctg ggaaaaacga ccttatgacg gtgacagaga acacaactta
     2581 tacgttgaat tcgatatgac attcagattt taaggataaa tatgttacgt aatggaaata
     2641 aatatctcct gatgctggtg agtataatta tgctcaccgc gtgcattagc cagtcaagaa
     2701 catcatttat accgccacag gatcgcaaat ctttactcgc cgagcaaccg tggccgcata
     2761 atggttttgt agcgatttca tggcataacg ttgaagacga agctgccgac cagcgtttta
     2821 tgtcagtgcg gacatcagca ctgcgtgaac aatttgcctg gctgcgcgag aacggttatc
     2881 aaccggtcag tattgctcaa attcgtgaag cacatcgagg aggaaaaccg ctaccggaaa
     2941 aagctgtagt gctgactttt gatgacggct accagagttt ttatacccgc gtcttcccaa
     3001 ttcttcaggc cttccagtgg cctgctgtat gggcccccgt cggcagttgg gtcgatacgc
     3061 cagcggataa acaagtaaaa tttggcgatg agttggtcga tcgagaatat tttgccacgt
     3121 ggcaacaagt gcgagaagtt gcgcgttccc ggctcgttga gctcgcttct catacatgga
     3181 attctcacta cggtattcag gctaatgcca ccggcagctt attgcctgta tatgtaaatc
     3241 gtgcatattt tactgaccac gcacggtatg aaaccgcagc agaataccgg gaaagaattc
     3301 gtctggatgc tgtaaaaatg acggaatacc tgcgtacaaa ggttgaggta aatccacacg
     3361 tttttatttg gccttatggc gaagcgaatg gcatagcgat agaggaatta aaaaaactcg
     3421 gttatgacat gttcttcacc cttgaatcag gtttggcaaa tgcgtcgcaa ttggattcca
     3481 ttccgcgggt attaatcgcc aataatccct cattaaaaga gtttgcccag caaattatta
     3541 ccgtacagga aaaatcacca caacggataa tgcatatcga tcttgattac gtttatgacg
     3601 aaaacctcca gcaaatggat cgcaatattg atgtgctaat tcagcgggtg aaagatatgc
     3661 aaatatcaac cgtgtatttg caggcatttg ctgatcccga tggtgatggg ctggtcaaag
     3721 aggtctggtt tccaaatcgt ttgctaccaa tgaaagcaga tatttttagt cgggttgcct
     3781 ggcaattacg tacccgctca ggtgtaaaca tctatgcgtg gatgccggta ttaagctggg
     3841 atttagatcc cacattaacg cgagtaaaat acttaccaac aggggagaaa aaagcacaaa
     3901 ttcatcctga acaatatcac cgtctctctc ctttcgatga cagagtcaga gcacaagttg
     3961 gcatgttata tgaagatctt gccggacatg ctgcttttga tggcatattg ttccacgatg
     4021 atgctttgct ttcagattat gaagatgcca gtgcaccggc tatcacggct tatcagcaag
     4081 caggctttag cgggagtctg agcgaaattc gacaaaaccc ggagcaattt aaacagtggg
     4141 cccgctttaa aagtcgtgcg ttaactgact tcactttaga acttagtgcg cgcgtaaaag
     4201 ccattcgcgg tccacatatt aaaactgcac gaaatatttt tgcacttccg gtaatacaac
     4261 ctgaaagtga agcctggttt gcacagaatt atgctgattt cctaaaaagc tatgactgga
     4321 ccgctattat ggctatgcct tatctggaag gtgtcgcaga aaaatcggct gaccaatggt
     4381 taatacaatt gaccaatcaa attaaaaaca tccctcaggc taaagacaaa tctattttag
     4441 aattacaggc acaaaactgg cagaaaaatg gtcagcatca ggctatttct tcgcaacaac
     4501 tcgctcactg gatgagccta ttacaactga atggagtgaa aaactatggt tattatcccg
     4561 acaattttct gcataaccaa cctgaaatag accttattcg tcctgagttt tcaacagcct
     4621 ggtatccgaa aaatgattaa tcgcatcgta tcgtttttta tattatgtct ggtgttatgc
     4681 atacccctat gcgtagcgta ctttcactct ggtgaactga tgatgaggtt cgttttcttc
     4741 tggccgtttt ttatgtccat tatgtggatt gttggcggcg tctatttctg ggtctatcgt
     4801 gaacgccgct ggccgtgggg agaaaacgca ccagctcccc agttgaaaga taatccgtct
     4861 atctccatta tcattccctg ttttaatgag gagaaaaacg ttgaggaaac catacacgcc
     4921 gctttagcac agcgttatga gaacattgaa gttattgccg taaatgacgg ttcaacagat
     4981 aaaacccgtg ccatcctgga tcgcatggct gcacaaattc cccatttgcg ggtcattcat
     5041 ctggcgcaaa accaggggaa agccattgcg cttaaaaccg gagctgccgc ggcgaaaagt
     5101 gaatatctgg tgtgcattga tggcgatgcg ttattagacc gcgatgcggc ggcatatatt
     5161 gtggaaccga tgttgtacaa cccgcgtgtg ggtgccgtaa ccggtaatcc tcgtattcga
     5221 acacgttcta ccctggtggg taaaattcag gttggcgagt attcctcaat tattggtttg
     5281 atcaagcgaa cccagcgtat ctatggaaac gtatttaccg tttccggtgt tattgccgca
     5341 tttcgtcgca gcgccctggc agaagtgggt tactggagtg acgatatgat caccgaagat
     5401 attgatatta gctggaagct gcagttgaat cagtggacga ttttttacga gccacgggca
     5461 ctgtgctgga tattaatgcc tgaaacgtta aaagggctgt ggaaacagcg cctgcgctgg
     5521 gctcagggcg gtgcagaagt attcctcaaa aatatgacaa ggttgtggcg caaagaaaac
     5581 tttcgaatgt ggccgctgtt ttttgaatac agcctgacga caatatgggc cttcacctgc
     5641 ctggtcggtt tcattattta cgcagtccaa cttgccggtg taccgttaaa tattgaattg
     5701 acacatatcg ctgcgacaca tactgccgga atattattgt gtacgttatg tttactgcaa
     5761 tttattgtca gcctgatgat cgagaatcgc tatgagcata atctgacttc atcgcttttc
     5821 tggattattt ggttcccggt tattttctgg atgctgagcc tggcaacgac attggtatca
     5881 tttacacgag tcatgttgat gcctaaaaag caacgcgccc gttgggtaag tcccgatcgc
     5941 gggattctga gaggttaata tgaacaattt aattattacg acccgacaat caccagtacg
     6001 tttactggtt gattatgttg ccacaaccat cttgtggaca ttatttgcgt tgttcatatt
     6061 cttattcgcc atggatctgc tgacgggtta ttactggcaa agcgaggcca gaagccgact
     6121 tcagttctat tttttgctgg cagtggcgaa tgccgtcgtg ttaattgtct gggcgctgta
     6181 caataagctg cgttttcaaa aacagcagca tcatgcagcc taccaatata cgccgcaaga
     6241 atatgcagag agcttagcaa tacctgatga gctctatcag caactacaaa aaagccacag
     6301 gatgagcgta cacttcacca gccaggggca aataaaaatg gttgtttcag aaaaagcgct
     6361 agtcagggca taaacaccca aaacaaagcc cggttcgccc gggctctgca ccgataatac
     6421 acttaactgt atgcatgcag cgtacgttgg caaagtgccg aacgtacgca atcctctttt
     6481 ccgaaccgga cgatcccaac catttcatct tcttcgaaac gttccagcgc gtcacttaat
     6541 ccggagcaca cgccgcgagg caaatcgcat tgcgtgatat caccgttgac gataaccgtc
     6601 acgttctccc cgaggcgggt taaaaacatt ttcatttgcg cggcagtcac attctgcgcc
     6661 tcgtcaagaa tgacgactgc attttcaaag gtacgtccac gcatataggc gaacggcgca
     6721 atttccacct tccctatttc cggtcgcagg cagtactgca taaaggaagc ccctaagcgc
     6781 cggaccagca cgtcgtagac cgggcgaaaa tagggagcaa acttttctgc gatatctcca
     6841 ggtaagaagc caagatcttc atcggcttgc agaactggac gggtgacgat aatcctgtcg
     6901 acatccttat gtatcagggc ctctgccgct tttgctgcgc tgatccaggt ttttccgcac
     6961 ccggcttcgc ccgtggcgaa tatcagctgc ttactctcaa tagccttcag atagtgcaat
     7021 tgcgcttcat ttcgcgcgag gatgggcgaa gtatcgcgac tgtcgcgggc cataccaatg
     7081 gcttctacgc cgcccatctg cacaagcgag gtgaccgatt cttcttcacg ctgcttatgg
     7141 ctgcgcgaat cccgtctcag cacacgtttt gcctcgcgac gagctttgat cactgctttt
     7201 tgtcttccca tggagagcac cttgagttgt ttgtattcat cacacgcgcc gttggcagcg
     7261 cgattatgcg cacgaacatc agagggttgg cttccttgta agccatagtt tgcttttgga
     7321 taaaatgccg aaaaacggct acgcgcaccg tttacggcgt cggtaacaca tgaaaagaaa
     7381 ggatgaggtt gaaaatgcaa agtgacgaga tgactaccgg aggagaaaac tccgcgagtg
     7441 gtggcgcgtt gattatctaa aacatgtcca gtacaggacg ttaccatccg cgatctccat
     7501 agtgactgac tatcactgcc gggaacttcc gctgctactt aataagtaca acagttctcg
     7561 catttattgc aacaatatat ttacttatat ttaactataa aacaccattt cagtgacatt
     7621 agtttctact ggaaagatga cagagtgatg acagatatga aaaaagctgt gtgctttcag
     7681 caggatttgc atcaggcatc agtgcacgat cgccgtatgc ggcgtgaacg ccttatccgc
     7741 ctaccgaacc cgcaccgatt gtaggcataa gaagacgcgc aagcatcgca tcaggcatct
     7801 gcgcaagaac gccttatccg tctaaaaaca ttaaacccgc aataacgcgc ttccgagata
     7861 atcgttcgcg tccttcactc ccggcagcgc aaaaaaataa ccgccgccga taggtttaac
     7921 gtattcctcc agcgcttcgc cattgagcct tttttgtact gtcaggaagc ctttttccag
     7981 atcgtgttgg taacagacaa acagtagccc catatccagt tgcccggagt tggtgacgcc
     8041 cagtgaataa ctgtagccac gacgcagcat caggctggac tcactctccg ccgtgcgggg
     8101 attcgccagc cggatatggc tgtccagcgc aatccccttc ccttccgggt cgctggcgta
     8161 atcaggcaca tcatgctcat gctgcattcc cagcggcgca ccggtttgct tatcacggcc
     8221 aaaaatcgtc tgctgttctt tcagcggcgt tctgtcccaa aattccactc gaaactgaat
     8281 caagcgtact gcctgatagc tgccaccgat tgtccacgca ggctcctgct gatctgccgt
     8341 tacccacacc actttttgca tcaacttatc attctggcta tcgggattag cagtgccgtc
     8401 tttgaaaccc agcaaattaa tcggcgtctc tttgccttta ctacgcgccg cgtgatcgga
     8461 aataaaccct tcccgcttcc agcgcacgct gagcaaatcc ggcgtatgtt tgatgatatc
     8521 gcgcagtgca tggatcaccg tgtcctgggt gttggcgcaa atttgtagca acacatcacc
     8581 atgacataac gccgcatcca gcgagtcgtt gggaaaacgc gtcatcttct gcaacttttt
     8641 cggcatctgt ggcgcaaggc caaagcgctc atcaaacaat gagtgaccca ccgataacgt
     8701 gatggtgaga ttatcgggcg caatgtagcc gccaagaatg ccggaatcga gtggtggcag
     8761 gcgcggattt ggcgtttctg gtgccgctcc gccctgagtc agaaaagcaa aacgctgagt
     8821 caacaagcga aacaaccgtt caagatcggc tttatcgctg gcaagcacat caaacgccac
     8881 cagcatcatt gcggcctgtt gtggcgtcag gatccctgcc tgatgctcac cataaaacgg
     8941 ctgtttctca ttgcgtgcaa ccggtgaaag cgtacccggc gcactttgcg ttttttgtgc
     9001 atgagcgacc ggacaacttc ccgccagcgc cagcgcacca atgcctttca gtaaacgtcg
     9061 gcgtgacggt tcattcacgc cgtttttatc ttcatactgc ataatgctta atccaatccc
     9121 agcacaccgc gaagttgcgc cagatcttcc gccagcgcag taatcggtcc tttcagtgca
     9181 ttccggtcgg catcggtcaa tttgtcgtag gtttcaaaac cgtctttagt acggtatttc
     9241 gccagaatgg tatcgacctt tttaaagttg gcatcgactt ttgccaacag ttccgggtta
     9301 gctttttgca gttgtggacg cagcagatcg acaattttct gcgagccttc aacgttagcc
     9361 tggaaatccc acagatcggt gtggctgtag cgatcttctt caccgctgat tttgctggct
     9421 gccacttcct caatcagtcc ggctgcaccg ccgaccactt ttgaaggtgg gaaagccagt
     9481 tcactgatgc gtttttgcaa atcgaccaca tcggtataaa gctggtcagc gtactgatcc
     9541 atgcctttgg tggtgttgtc gccaaacaat gctttttcca gacggtggaa accggtgaat
     9601 tttggatcag cggctttttg ctcgtaatca tcttcacggg cgtcaatgct gccatccaga
     9661 tcggagaaca gttcagcaat cggttcaatg cgctcatagt gctggcgcgt cggcgcatac
     9721 agtgctttcg ctttttcgat atcgcctgct ttaatcgcgt cggtaaaggc tttggtgtcg
     9781 gtcaccagct gtgtggtttc cgccatgaca tacgctttat atgcagtaat tgcaccacca
     9841 agacttaaca gcgcatcact ttgcgccgca tccgccgttg cctcaccttt gacgatcaac
     9901 ttccctttcg ggttagtcag cagaccgcag gtcatatcgt attcgccagg ctgtaaattc
     9961 gccgtcattt tctggctaaa gccaggggcg atattttccc gctcttccac caccatcacg
    10021 cctttgagga tctcccattc cagcgccttc tggctgtggt tctgaataat gaactgtgtt
    10081 ttcccggcgt taaccgtaat ggtcatcggt tcgcactgct tatccgtcac ggtcactttg
    10141 acctgcggca catcagcggc gttagccata aaagcagaag aaaacagcgc agccacgctc
    10201 aactgcaatg cgttacggcg gaagttaatg gtcatgacga gactatccct ttaaagtatg
    10261 ttgtaactaa gcaagagttt gcgtcgtatt tgttacgcgg agcgagacgc tgtcgcccct
    10321 gcgcgtggtg gcagagcaaa tgccaccagc gccgggatga gataaataaa ccagacggcg
    10381 acttcgctga cgctcggcgc ttcctgatag ccaaaaatcc cttccatcag cgtgccaaac
    10441 agcgagtgag ttgagagcac cgcactcata tcgaaggcga tttcctgaaa gtggttccac
    10501 aatccggctt catgaaatgc gcgaatggca ccagctgcca gccctgcggc gacgaagaga
    10561 ataaacaggc tggtccattt aaaaaatgca ccaagattga ggcgaatacc gccccagtag
    10621 agcaggaagc ctagcaccac ggcagtagca agaccgagca ttgcacccag cggcggccag
    10681 atcccgacat cttgttgaaa tgccgccagc aggaaaaaga ccgactccag cccttccctt
    10741 gcaacggcaa aaaagaccat catcaccagc gcccagccat gatgatttcc acgctgcaat
    10801 gcgctatcga cagcctgctc cagttgtact ttgacgttgc gcgacacttt gcgcatccag
    10861 aaaaccatcc aggtaaggat caccacggcg atcaccgcca cgataccttc aaacagttcc
    10921 tgctcttttt gcggaaattc gccggtggtt tcgttaatga agatccccaa gcccaggcac
    10981 aacgcagcgg caagcaacac gccaatccac atcacaccaa tccatcggcc tcgctgggta
    11041 cgcttaagat agctggcaat caaactgaca atcagcgcgg cttcaagtcc ttcgcgcaac
    11101 ataatgagaa acggaacaaa catgccaccc acccttaaac gtttttctga gtcaatctat
    11161 gcaaagaaaa gtaaatcgca gtgata
//
LOCUS       DBFIFM010000150        11176 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.150, whole genome shotgun sequence.
ACCESSION   DBFIFM010000150 DBFIFM010000000
VERSION     DBFIFM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.150"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(114..380)
                     /locus_tag="N7C74_003472"
     CDS             complement(114..380)
                     /locus_tag="N7C74_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HGU2433275.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTEAIYCQQGSDWQCVVHYDAEASTRQ"
     gene            complement(377..1144)
                     /gene="yjiL"
                     /locus_tag="N7C74_003473"
     CDS             complement(377..1144)
                     /gene="yjiL"
                     /locus_tag="N7C74_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HGU2433276.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDCITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLRMPVNTHPDAQFAGTIGAAVIGQRVRTRR"
     gene            complement(1154..2305)
                     /locus_tag="N7C74_003474"
     CDS             complement(1154..2305)
                     /locus_tag="N7C74_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HGU2433277.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKVRGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKAVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQFNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARIRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVVETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            complement(2421..3692)
                     /locus_tag="N7C74_003475"
     CDS             complement(2421..3692)
                     /locus_tag="N7C74_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HGU2433278.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQKKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMTARADAVKSYLKEDEAFLSELWGDLREWLKADINSEDSRVKERIARAGQWFGETLI
                     ADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKDLQF
                     IRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            complement(3733..4965)
                     /gene="mdtM"
                     /locus_tag="N7C74_003476"
     CDS             complement(3733..4965)
                     /gene="mdtM"
                     /locus_tag="N7C74_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HGU2433279.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRKPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEEQ"
     gene            5432..6214
                     /locus_tag="N7C74_003477"
     CDS             5432..6214
                     /locus_tag="N7C74_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006685483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HGU2433280.1"
                     /translation="MTNFTTSTPHDALFKSFLMHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYNAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEERFNEFISELTSRMPQHRERIMTIAERIHNDDCRANS"
     gene            6189..6389
                     /locus_tag="N7C74_003478"
     CDS             6189..6389
                     /locus_tag="N7C74_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433281.1"
                     /translation="MTIAERIHNDGWLLGREEGEQRILRLLLQNGADPEWIQKITGLS
                     AEQMQALRQPLPERERYSWLKS"
     gene            complement(6632..8044)
                     /locus_tag="N7C74_003479"
     CDS             complement(6632..8044)
                     /locus_tag="N7C74_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGU2433282.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDLVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQVARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVTRQLCRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            8221..8385
                     /locus_tag="N7C74_003480"
     CDS             8221..8385
                     /locus_tag="N7C74_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HGU2433283.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQARRVLQQMSDERLR
                     DIGLRREDVE"
     gene            8482..9363
                     /locus_tag="N7C74_003481"
     CDS             8482..9363
                     /locus_tag="N7C74_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HGU2433284.1"
                     /translation="MKATEARLLDFLKRSQQFVIPIYQRTYSWTEQQCRQLWDDIIRA
                     GKRDDISAHFIGSVVYIEQGLYQVSGISPLLVIDGQQRLTTAMLLIEALSRHLGEDEV
                     FDGFSAMKLRNYYLLNPYESGEKGFKLLLTETDKDSLLALIKQRPMPENYSHRIMENF
                     TFFDEQIAKLGDDLIPLCRGLAKLLIVDVALNRGQDNPQLIFESMNSTGKALSQADLV
                     RNFILMGLEPEHQTRLYEDHWRPMEVACGQQGYSEYFDSFMRHYLTVKTGRSLGQMKS
                     MRHLNSMPAARVLLKKA"
     gene            9411..10574
                     /locus_tag="N7C74_003482"
     CDS             9411..10574
                     /locus_tag="N7C74_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HGU2433285.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNDDLSH
                     EDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVINKEKYLESIQVHFLNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRIHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIHARADRLAELAVQVWQAPSLPEEVLAVYRGQPENKTSYSLSDYPFLAD
                     GSHSRVLFDHLRDEIMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
     gene            complement(10621..10962)
                     /gene="symE"
                     /locus_tag="N7C74_003483"
     CDS             complement(10621..10962)
                     /gene="symE"
                     /locus_tag="N7C74_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HGU2433286.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESELMQSLRQVCKLSARKQRQVQEF
                     IGVIAGKQKVA"
BASE COUNT         2920 a         2785 c         2829 g         2642 t
ORIGIN      
        1 aaatcgctca ttttgtggat gggaaatatt tcacggcagg cgagatattt actacaactt
       61 ccttcatgca ggattatgcg cagccaatta tccggttggt atatttaaag atcctattgc
      121 ctggttgacg cttctgcatc gtaatgcacc acgcactgcc agtcgctgcc ctgctggcaa
      181 tatatcgctt cggtttgccc agggatcacc cattgtattt cctcgccggg gggacaggtc
      241 agccaaatgc ataacccgca gccgccgcgt aaatcacggg gaatatcgct gacgcgaaaa
      301 gtcatgcccg ctgcctgcaa cgccttgcgg gtttgtatga cgccgaccgt ggagtgaaat
      361 aaaaataaaa actctttcat cggcgtgtcc tcactcgttg accaattacc gccgcgccaa
      421 ttgtgccagc aaattgcgca tcaggatggg tatttaccgg cattcgcagg tgagattcca
      481 gcatccgggc aaacgtctgg caatgactaa cgccaccagt aaacagaatc ggcgcttcac
      541 aggagagacg agcaatgaaa ttggcactcc gccgcgccat cgcgttaatc actccggcga
      601 gaatcgcttc tggcgcgacg cccgctgagc gcaggctgat cacttctgat tcagcaaaca
      661 ctgtgcacat actcgtgatg gcgtgcggcg tgacattttc ggtaatgcag tcgagttgct
      721 cgacgctggt gccaagcgtg cgcgagatca cctccaggaa acgcccggtg cccgccgcgc
      781 atttgtcatt catcaggaaa tcgcacaggt taccgtcatc atcaagctga atcactttgc
      841 tgtcctgacc accgatgtcg attaccgcgc gcgttgctgg cgcaagaaac cgtgcgccca
      901 gcccgtgaca ggagatttcc gtcacttgtt tatcggcaaa atccaccagt tgccgcccgt
      961 agccggtgag cgtcagaaac ggcgttgtct ctaacccttc gcgcagagtt tcccaggctt
     1021 cagtaattgc tgttgccggt cgaaagggtg ttggaacgag gaaacggcgc gtaatcacgc
     1081 cgtctgcgag taagatccct ttggtggcgg tagaaccaga atcaatgcca atcgaatatg
     1141 tcactgctac tccttacagc atctcaataa aggccgcgac acgggtactg agctgcccaa
     1201 catccgaggt ggagtagtct gtttcaatag cgatataagg aatgttgtgc tgctgacgca
     1261 catgacgttt aattgccagc gattccaccg cgtaggtatg gcacgcctgc aaaatcacat
     1321 caactacgcc atcgacctga tattcttcca ccatctggct gagcattttc aggcgctgat
     1381 cgttcggcga aacacaggag cagccaatcg ccagatattt atccgccagc gcgtcgtaga
     1441 catcgcccgt ttctaccacg cattgctcgg tcgctttcgc cccggtgcag ttttcataac
     1501 cgacaaccca gccgccattc tcttcaatcg cgcgcaccac tttctctgct gcgccgccaa
     1561 tcgggcagcc ggtgattaaa atgcgcggac gcgggtccag tcgctggcct tcttcccact
     1621 gctgacgaat gcgggcggtc atcgcgtcca gttcattgat caacgcctct ttatcgaacc
     1681 ggaaggttgc gccgtaaacc actttcagaa tgtcgctgcc gctaagcgcc ggaggattga
     1741 actgcccaag atgataaaaa ttggccagtg cgcgacgttc gcggtttttc agcgcaatgg
     1801 catcgcgcag agcatcttcg ctaatttcgt gcccaaaacg ttcttccacc gctttttgta
     1861 agcgcagcat ctcggctttc cataacgcac gcgaggcatc gtctttaacg ctgtttggca
     1921 gctgcatcac atgaacgggc ttaaactccg ccatgtattc atacattttc tttttgccgt
     1981 cgcaggtggt ttcaccgacc accagatccg aaaagtagaa gtaggggcat ttatcggttt
     2041 tgccgaagcc gtagctactt ttaatcagcg ggcagaggtt gcgcggcaga tctttctccg
     2101 cttcttcaat ggtttcatca gaggtggaac agagcgaaac cacaaccgca ccggctgcca
     2161 tcgggatctc ttgcggcata aaggtgcagt aagtgccaac cagcggaatg ccgcgcacct
     2221 tgagatccat gacggtgaga aagcctttct ggcgagcttc agagaactga tcgaaaatgg
     2281 cgggtagatc ggtgacaagt gacatgattt tccttccccg taccacggga gataatgaaa
     2341 agaggccaca ttattaccat tcttttagta tggtttattt gatctctctc gcggttagcc
     2401 acttagtttt tcatggattt ctaaaaattg ccgaggggga acaaggccgg gagctgtgac
     2461 agcaaatata aaattagccc aatacaaccg ccaaccagcg taccgttgac acggataaac
     2521 tgcagatctt tgccgatatt taactctatt tgccgcgaca tatcccgggc atcccagctt
     2581 tttaccgtat cgctgatatg gcgcgtcagg aatgcagaaa actcaggcgc gacgcggtgc
     2641 gcggcttgtt ccaggtgacc atttaacgac gcccgcaagg catcatcggc aattaacgtt
     2701 tcgccaaacc attgacctgc ccgcgcgata cgttctttca cacgagaatc ttcactgttg
     2761 atatccgctt tcagccattc ccgtaaatcc ccccacaatt cactgagaaa ggcttcatcc
     2821 tctttcagat agctttttac ggcatcagct cgcgctgtca tttcaggatc gtttttcaac
     2881 ttgtcgatca gggcaaaagt ggcgcgatca aatgcatggc ggatctgatg cgcacgatcg
     2941 cggctgatat catcaagcaa agaattcacc gcgtcagaaa ccaactccgc gctatgttcg
     3001 cccagccatt cggtggggag aattttggct ttcagtggat gctcactctc cagccagcga
     3061 acaatctgct gggcgataaa cttgcgcgat ttatcgcgct ggagaagggc gatcaactgt
     3121 gcgatcagcg tatccagcag cacctgatga cgatcgtttt tggtcatact ctccagcatc
     3181 aacgcactgg tgccggaaag atcgacctta tcaatcgccc gatggaccgc gcgcttaagc
     3241 aggcgctgaa tacgtgcatc atcggtcagt tcaagaaaac cactcatgat ctgcaacaga
     3301 tgctgaccaa cgcggcgggc gttttctggc tggctaaacc agttgccaat cagcaacgcc
     3361 ggttcgtgac gtcgaatcaa tgccaccagc gattgggtat cgagaaattt tttctgcacg
     3421 aactgaccga gattttcgcc aatccggtct ttattacgcg ggataatcgc cgtatgacga
     3481 gaaatgatcg gaatcggcac gcggcgaaac agcgccacca ccgcaaacca gtccgccagc
     3541 gcgccgacca tcgccgcttc agcaatcgcc ttcacgccgc tcacccaaaa attgggcggc
     3601 aaaaacagcg taacgacaaa ggtagcagcg gcgataagca gtaaagagag cgccaacatt
     3661 ttggcgcgtc tgagttcaat gagtttattc ataacgttaa gaatagagcc tgacggcagg
     3721 atcgcgcaaa aatcactgct cctctgccag ctcggcagcc tgatgttggc gcacgcgatt
     3781 aagcaatccg gccagggtga aaacgacgat aacgcccgcc acaacggcta acagatgaaa
     3841 cggcaagcga ccgccgttaa accacagcca gcggccaatt tcgaccgaga cagacatcac
     3901 catcaggatc accatattca gcgatgcgga gacggtccct ttcggtaagt tattggaaaa
     3961 cagcgtaaag cggaataagg tcgggaagat caaaccaatc ccgaaagcat acagactggt
     4021 gcccagcacc gaccacagcc agacgtgcgg tgacagcaga ttgccgataa tcaacagcgc
     4081 gaggccgacc agttgaatgg gtacggcacg ccagataaag cgcggttcgg tcggatcttt
     4141 aacgaaacgc gccacgatgg cattcgcgac aatcaccgcg ccgaacaccg gaacctgtgt
     4201 ccaggcgaac tgcgaggtcg ttaagccgcc tgcgtcaatc aggatcaccg gcgagacagc
     4261 cacccaactc atcatgggga tatagcttaa agagatggtt gctgcgccaa agaggaacag
     4321 ccgattgcaa aagacattac gaaaatcgcg caagacgctt ttggcgctaa atggaaccgc
     4381 gccgcgcttc accgtctctg gcatcgccag cagtaagcca acaaatgaga taaaacccat
     4441 aaccgcaatg atggcaaaaa ggactttcca gtgcacaaag tgcatcagag ctgcgccgga
     4501 aagcgggccg ataatcggcg caatcagtac gatggaggtg ataatcgcca tcaacttgat
     4561 cccttttgtc tggccgaacg cctcctgcac cgtgacataa ccaacggtgg caataaaaca
     4621 gatactggtg ccctgaattg cacgcgcgat aagaaactgt gtcatagacg ttgtgaacat
     4681 tgtcgcagcg caggcaaggg taaatattag cgccccggta atcagcactg gcttgcggcc
     4741 aatcctgtcg gaaagcggcc ccagcagcca ctgcaacgcc atgcctccag cgagatagag
     4801 actgacggca gccggagcca gactgacatc ggcattaaaa tcacggacca cattaatgat
     4861 cccaggctgg atcaggtccg tcgaaagata ggcggcaaag tcatacaaaa tcaacgccat
     4921 cgggaaaaac agcgtggcgg catggcgggc aaaaaaacgt ggcataacaa acaactcctt
     4981 gtccggagaa ccccaacggg gaaaacgaaa ttcaataccg tcggtaatga cgaaaggtag
     5041 gcgagaagga caaatgtact gaatttcatg taatcaacca cactgcctgt gaacacacag
     5101 acagagaaat accttctgac ttcgggtcag aatgtataac gattacaaat agtcatcagc
     5161 acattacacc tcttgaattg atgggttgca tagaaaatat acaccttaag tgtaattaaa
     5221 atttgcgatt aatcaaaaga gcgcggtgga agggggaata tctgccgggt acaggacatg
     5281 taaaacgcga gtcagtttcc ggaaaaatgt atcgtttgca gacgaacaaa aatagcctgg
     5341 aaagcgcctc gggaaacgag aaattgccgg gtgagaatgg ttttgttagt cgctaaagtc
     5401 gggccatctt atcaacaggt gacggatcgc catgacaaac ttcacgacca gcacgccgca
     5461 tgacgcatta tttaaatctt ttctcatgca ccctgacacc gcgcgggatt ttatggagat
     5521 ccacttaccc aaagatttac gtgaactgtg cgatctcgac agcttaaaac tggaatccgc
     5581 cagctttgtc gatgaaaaat tgcgggcgct acactccgat attctgtggt cggtaaagac
     5641 ccgggaaggc gatggctata tctatgtggt gattgaacat cagagccgtg aggatattca
     5701 tatggccttt cgcctgatgc gctattccat ggcagtgatg cagcgccata tagagcatga
     5761 taaacgccgg ccactaccgc tggtcatccc gatgctgttt tatcacggta gccgtagtcc
     5821 ttatccctgg tctttgtgct ggttggacga atttgccgac ccgactactg cacggaagct
     5881 ttataacgcc gcgttcccgc tggtggatat cactgtcgtg ccggacgacg agattgtgca
     5941 gcaccgcaga gtcgccctgt tggagttgat ccaaaagcat attcgccagc gcgatctgat
     6001 ggggcttatc gatcaactgg tagtattact ggttacagag tgtgctaatg acagccagat
     6061 aactgcgctg ttaaattaca ttttactgac tggcgatgaa gagcgtttta atgagtttat
     6121 cagtgaacta accagtcgaa tgcctcaaca cagagagcga ataatgacga ttgcagagcg
     6181 aattcataat gacgattgca gagcgaattc ataatgatgg atggctgttg ggaagggaag
     6241 aaggggaaca gcgcattctt cgattgttgt tgcagaatgg ggcagatcct gaatggatac
     6301 agaagattac cggactttcg gcagagcaaa tgcaggcatt aaggcagccc ttgcctgagc
     6361 gtgagcgcta ttcatggctc aagagctaac cagagacgga tgacaaacgc aaagcagcct
     6421 gatgtgctac gcttatcagg tctacaaagc ccattcaata tattgaactt taaagatttt
     6481 gtagacctgg taaggcgttc acatggcatc cggcatgaac aaagcgcact ttacctaagc
     6541 catgccggaa cgtggctatg atcctgcaaa gataaaacga gtcacaggcg attcgtgaga
     6601 ataaatcagg tgcttcgcca ctaattctaa tttattccat tgcccgatac acggcctcgc
     6661 caatttgctt cagcgcttcg cgataagttt cgctgagtgg caaagcacag ttgatgcgca
     6721 ggcagttacg gtatttgccg gaagctgaga aaatcgagcc tgccgccacc tggattttca
     6781 tgcggcacag ctgccgcgtg acgcagacca tatcgacctg ttcaggcaat tctatccaca
     6841 gtaaaaatcc gcctttcggg cgcgtaatac agatttcgca gggaaaatat tcccgtatcc
     6901 agcaggtata aagcgccaaa ttgcgctgat agatctgccg catccgccgg atatggcgat
     6961 gatagtgacc ttccagcaca aacgttgccg ccgccatttg cgtggacggc acattaaagc
     7021 tgctgatggc gtatttcata tgcatcagtt tatcgtgata acgccccggt gcgacccaac
     7081 ccacgcgcag gcctggagca atacttttac tgaacgagct gcacaacagc actcgcccgt
     7141 cgatatccca ggaatgaatg gtccgcgggc gcggatactc cgttgccagt tcgccgtaga
     7201 catcatcttc aaaaatcaca atatcatgac gctgagcgag agagagaacg gcccgtttgc
     7261 gcgcgtccgg cataataaat cccagcggat tattacagtt tggcaccaga atgatgcctt
     7321 taatcggcca ctgttccagc gccagttcca gcgcttcaac gctgatgcct gtttctggat
     7381 cggttgggat ttcaatcact ttcacgccca tgccgcgcag catctgcatt gaaccgtaat
     7441 aacaggggga ttcgaccgcg acaatatcgc ccggtttaca caccgccatt aacgctagcg
     7501 acatcgagtt atggcagccg ctggtgatga tgatgtcatc ggcagtgacc accgagccgc
     7561 tgtcgagcat caggcgggca acctgctcgc gcaatacccg ctgaccggct agcaagtcat
     7621 aaccgagaac ggtttgcaga ttatgctgca ccacccggct tagctcccgc cacagcggtt
     7681 tcaggctggg cgtttcgaca tccggcgtgc ttttgcttaa cggaacaatg gaactgtcgc
     7741 tatgcgccac cagcatatcc agcaccagat cccactgggt aatttccact gggcgctgca
     7801 ccggacgcgt catcggcggc actggcggct gggctttacg ttgtgcgaca aaataacccg
     7861 aacgcggctg cggagtgatg agcttcatcg tctccagcgt ctggtacgcc tgctgcacgg
     7921 tgctgatgct gacgccgtgc tcctgactta agctgcgcac cgacggcaat ttctccccgt
     7981 gacgatacag cccttgctca atccgctcgg caagtagagt cgccagatgt tgataacgcg
     8041 tcatgctgta ttccttattt ggaccataca gaaagaaaaa ccggtacaga tgaggctaaa
     8101 aaaccggatg cagatgctgt tttaacctgt ctgtatgtta aacaaaacta atatttgcat
     8161 ctgtattgtg aactgccaga agacggatga tgaagcctct gaaatatgag gaggcagcag
     8221 atggaatttc acgaaaacaa agccaaaacg ccgtttatca gacttgtgca actctggcag
     8281 gcagtgaggc gttggcggcg gcaaatgcag gcccgacggg tgttacagca gatgagtgat
     8341 gaacggttga gggatattgg gttacgcagg gaggatgtgg agtgaggggt atgttcgtcg
     8401 cggataaaca ggatttctct ataacctcca taaggaagtg gttattattg cgaaatcatc
     8461 gtttcacgga aagacgtcag aatgaaagct acagaagctc gcctactcga ttttttaaaa
     8521 cgttcgcagc agtttgttat tcccatttat caacggactt attcatggac agaacaacaa
     8581 tgtcggcaac tttgggacga catcattcgt gcaggaaagc gtgacgatat atcagcgcat
     8641 tttatcggtt cggttgttta tatagagcag ggattgtatc aggtttctgg tatttctccg
     8701 ttgctggtca ttgatggtca gcagcggctg acgaccgcaa tgttgctgat tgaggcttta
     8761 tcgcgccatc ttggcgaaga cgaagttttt gatggctttt cggcaatgaa attgcgtaat
     8821 tattatttgc tcaatcctta tgagtccggc gagaaaggtt ttaaattact gctgaccgag
     8881 actgataaag acagtttact ggcgttaata aaacaaagac caatgccaga aaactattcc
     8941 catcgaataa tggaaaactt tactttcttt gatgaacaaa ttgccaaact cggtgatgac
     9001 ttgatcccct tatgtcgtgg gttagcaaag ttattaattg tcgatgtggc gcttaatcgt
     9061 ggtcaggata atccgcaact gatttttgaa agtatgaact ccaccggtaa ggcgttaagt
     9121 caggccgatc tggtgcgcaa ttttattctg atgggcctcg aaccagagca tcaaacccgg
     9181 ttgtatgaag atcactggcg tccaatggaa gtcgcttgtg gtcagcaagg ttacagcgaa
     9241 tattttgaca gttttatgcg tcattatctg acggtaaaaa cggggagatc cctcggacag
     9301 atgaagtcta tgaggcattt aaactccatg cccgcagcca gagtgttgct gaaaaaggcg
     9361 tagatcggct ggttgaagat attcatatct acgcggagta ttactgtgca atggcattgg
     9421 gaaaagaaag tgacaaatcg cttgctacgg cttttcagga tttgcgcgag ttaaaggttg
     9481 atgtggcgta tcctttctta ctggcgcttt atcatgacta taaaaatgac gatttgtctc
     9541 acgaagattt cctgagcata attcgtttaa ttgaatctta tgttttccgc cgtgcagtat
     9601 gtgcgattcc gacaaattct cttaacaaga cgtttgctac tttttataag gtcattaata
     9661 aagaaaaata tctggaaagt attcaggtac attttttgaa tctaccttca tatcgtcgtt
     9721 tccccaacga tgatgaattt aaacgggaat taaaagttcg cgatctctat aacttccgta
     9781 gtcgtagcta ctggttacga cgactggaaa acgataaacg cagagagcgc gtggaagagt
     9841 ttacgattga acatattatg ccgcagaacg aaaatttgtc ggctaaatgg cgcgaagagc
     9901 tgggaagtga ctggcagcgt attcataaag aactgttgca tacgttgggg aatctcactt
     9961 taacgcgcta taactcccgt tacagtgaca gacctttcgc agaaaaacgc gatattgaag
    10021 acggctttaa gcatagcccg ctctatttga atatcggtct tggacagtgc gaaaaatggg
    10081 atgaagctgc cattcacgcc cgagccgatc gtctggcgga gcttgcggtt caggtctggc
    10141 aagcgccttc tcttcctgaa gaggttttag ctgtttatcg gggacagcct gagaacaaaa
    10201 ccagttacag cctgagtgat tatccttttc ttgctgatgg ttcgcatagc cgggtgttat
    10261 tcgatcatct tcgcgatgaa attatgcgcc tggacgcagg gatcacgcag gaagtattga
    10321 agctctatat tgcgtttaaa gcggaaacga attttgttga tgttgtgccg caaaaaagcc
    10381 gactgcgatt gtcgcttaat atgcagtttc atgaactggt cgatccgaaa ggtattgcca
    10441 aagatgtgac aaatgttggg cgatggggca atggtgatgt ggaaattggt ttcagtgacc
    10501 tcgcacaact tccttacatt atggggttaa ttcgtcaggc atttgaaaaa cagatggaga
    10561 gcgcgttggt ataacgcagc gaaaaatgcc cggtagtcct ggcgctaccg ggtcaatgta
    10621 tcaggcaact ttctgtttac ccgcaatcac cccaataaac tcctgcacct gcctttgttt
    10681 acgcgccgac agcttgcaca cctggcgcag cgactgcatc agttcgctct cggcggcggc
    10741 gggtggttgg gcggtgagga caatacagcc ttccatcact ttgacatcta ccaccgtgcc
    10801 ggtggcaaaa ccggcggctt ccagccactg acctttcagg gtgatggcgg gaatacggct
    10861 gtaatccggg taacgactcg cataactcac ggttaattga cggttatttg ccggggagac
    10921 ttctgcttcg aacggttgtg caatagaatg cgtatcagtc ataactgcta ttccctataa
    10981 atagtgattg tgattagcgg tgcgggtgtg ttggcgcaca tccgcaccgc gctaaatacc
    11041 tggatatatc atcagtaaat atggggaaag tccagctaaa aattaatgaa aataagatga
    11101 aaattagcgt gaaatataaa ttttggtgat gtgaataaag cggatattta gccgctttat
    11161 tggtttttcg agggca
//
LOCUS       DBFIFM010000151        11041 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.151, whole genome shotgun sequence.
ACCESSION   DBFIFM010000151 DBFIFM010000000
VERSION     DBFIFM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.151"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(101..1930)
                     /gene="glmS"
                     /locus_tag="N7C74_003484"
     CDS             complement(101..1930)
                     /gene="glmS"
                     /locus_tag="N7C74_003484"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HGU2433287.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFAEQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2092..3462)
                     /gene="glmU"
                     /locus_tag="N7C74_003485"
     CDS             complement(2092..3462)
                     /gene="glmU"
                     /locus_tag="N7C74_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HGU2433288.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGSGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3814..4233)
                     /gene="atpC"
                     /locus_tag="N7C74_003486"
     CDS             complement(3814..4233)
                     /gene="atpC"
                     /locus_tag="N7C74_003486"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HGU2433289.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4254..5636)
                     /gene="atpD"
                     /locus_tag="N7C74_003487"
     CDS             complement(4254..5636)
                     /gene="atpD"
                     /locus_tag="N7C74_003487"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HGU2433290.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5663..6526)
                     /gene="atpG"
                     /locus_tag="N7C74_003488"
     CDS             complement(5663..6526)
                     /gene="atpG"
                     /locus_tag="N7C74_003488"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HGU2433291.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6577..8118)
                     /gene="atpA"
                     /locus_tag="N7C74_003489"
     CDS             complement(6577..8118)
                     /gene="atpA"
                     /locus_tag="N7C74_003489"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HGU2433292.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8131..8664)
                     /gene="atpH"
                     /locus_tag="N7C74_003490"
     CDS             complement(8131..8664)
                     /gene="atpH"
                     /locus_tag="N7C74_003490"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HGU2433293.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8679..9149)
                     /gene="atpF"
                     /locus_tag="N7C74_003491"
     CDS             complement(8679..9149)
                     /gene="atpF"
                     /locus_tag="N7C74_003491"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HGU2433294.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9211..9450)
                     /gene="atpE"
                     /locus_tag="N7C74_003492"
     CDS             complement(9211..9450)
                     /gene="atpE"
                     /locus_tag="N7C74_003492"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HGU2433295.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9497..10312)
                     /gene="atpB"
                     /locus_tag="N7C74_003493"
     CDS             complement(9497..10312)
                     /gene="atpB"
                     /locus_tag="N7C74_003493"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HGU2433296.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10321..10701)
                     /gene="atpI"
                     /locus_tag="N7C74_003494"
     CDS             complement(10321..10701)
                     /gene="atpI"
                     /locus_tag="N7C74_003494"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HGU2433297.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2639 a         3010 c         2742 g         2650 t
ORIGIN      
        1 taatagctga agttaattat atggaatgag gttagattat taatgctggt aagtaaaagg
       61 gccgccaaaa ggcagccctt cgttcagcaa atgaataata ttactcaacc gttaccgatt
      121 ttgccaggtt acgcggctgg tcaacgtcgg tgcctttgat cagcgcgacg tgataagcca
      181 gtaactgcag cggaacggtg tagaagatcg gtgcaatcac ctcttccaca tgcggcatct
      241 cgatgatgtg catgttatcg ctacttacaa aacccgcatc ctgctcggcg aaaacataca
      301 attggccgcc acgcgcgcga acttcttcaa tgttggattt cagtttttcc agcaattcgt
      361 tgttcggcgc aacgacgata accggcatat cggcgtcaat cagcgccagc ggaccgtgtt
      421 tcagttcgcc tgcagcgtag gcttcagcgt gaatataaga gatctctttc agcttcaatg
      481 cgccttccag cgcgattggg tactgatcgc cacggcccag gaacagcgcg tgatgtttgt
      541 cagagaaatc ttctgccaga gcttcaatgc gtttgtcctg agacagcatc tgctcaatac
      601 ggctcggcag cgcctgcaga ccatgcacaa tgtcatgttc aatggaggca tccagacctt
      661 tcaggcgaga cagcttcgcc accagcatca acagcacagt taactgagtg gtgaatgctt
      721 tagtggatgc cacgccgatt tccgtacccg cgttggtcat cagcgccaga tcggattcgc
      781 gcaccagaga agaacccgga acgttacaga ttgccagcga accaaggtaa cccagctctt
      841 tcgacagacg caggcccgcc agggtatccg cggtttcgcc agactgtgac aaggtgatca
      901 tcaggctgtt acgacgcacg gcagatttac gatagcggaa ttcagaggcg atttcgacgt
      961 cgcacggaat acctgccagc gattcaaacc agtaacggga aaccataccg gagttataag
     1021 aagtaccaca ggcgaggatc tgaatatgct caaccttcga cagcagttcg tcggcgttcg
     1081 gtcccagctc gcttaaatca acctgaccgt ggctgatgcg cccggtaagg gtgtttttga
     1141 tcgcgttcgg ctgttcgtag atctctttct gcatgtagtg acggtaaata cctttatcgc
     1201 ccgcgtcata ttgcagattg gattcgatat cctgacgttt tacttccgcg ccagttttat
     1261 cgaagatgtt taccgaacgg cgagtgattt ccgcaatatc gccctcttca aggaagataa
     1321 agcgacgggt caccggcagc agcgccagct ggtcagaagc gataaagttt tcacccatac
     1381 ccagaccaat caccagcgga ctaccagaac gtgctgccag cagggtatcc gggtgacggg
     1441 agtccatgat cactgtaccg tacgcaccac gcagttgcgg gatagcacgc agaacagcct
     1501 cacgcagagt accgccctgt ttcagctccc agttcaccag atgggcaatc acttcggtgt
     1561 cggtttcaga aacgaaggta tagccacgcg cttttaactc ttcgcgcagc ggttcatggt
     1621 tttcgatgat gccgttatgc accaccacaa tgtgttcaga aacatgcgga tgcgcattca
     1681 cttctgaagg ttcaccgtgg gtcgcccagc gagtgtgagc aataccggtg ccgccatgca
     1741 gaggatgttc ttccgctgcc tgagccagca tctggacttt accgaggcga cgcaggcggg
     1801 tcatatgacc ttccgcatca acaacggcca gaccggcaga gtcatatccg cggtattcca
     1861 gacgacgtaa accttcaaga aggatttctg ctacatcacg ttgcgcgatc gcgccaacaa
     1921 ttccacacat agtttttgat tccgatttat atcgttgtcg gtcaacctgt atgcccgttt
     1981 tttcgggcgc cccgagcctt gtagagagtg gggttatttt tatagttact gcttgtggga
     2041 gggagattat gttatctcct catcccatgt gaccggatta cccggccaga atcacttttt
     2101 ctttaccgga cgacgccagc cttctttctg agtctgcggc acacggctga tagctaatgc
     2161 attttcgccg acattacgcg tcacagttgt acccgcagca atggtcgcgc ctttgcctac
     2221 tgttaccggg gccaccagct gagtgtcgga accgacaaac acatcgtcgc cgataatggt
     2281 cttaaattta ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga tgttaacgtt
     2341 atcgccaatt tccgcatcgc caaggtaagt cagatgacca gctttcgagc ctttacccag
     2401 acgtgctttt ttcatctcaa caaagttacc gacgtgtgca ccttccaaca actcagcacc
     2461 aggacgcaaa cgggcaaacg ggccaatggt acaggccgcc gccagattcg cgtcttccac
     2521 gacggtatat gggctaattt cgcaatcatc gccaatcacg ctgtttttaa tcacgcaacc
     2581 ggagccgatt ttcacgcgat ggccgagagt cacgttgccc tcgatgataa cgttagtatc
     2641 aatttcaaca tcgcgcccgt gagtaagcgt accgcgcaga tcaaaacgcg ccggatcgcg
     2701 cagcataacg cctgctaaca gcagtttttc agcctgttcg gactggtaaa cgcgctccag
     2761 acgggagagt tgcaggcggt tattcacgcc ttctacttcg cttaaacgtt gcggatgaac
     2821 ggcgacgatt tcacgccctt cctgatacgc cagcgcaata atgtcggtga tgtagtattc
     2881 gccctgcgca ttattgttgg tcagcttcgc cagccagcgt ttcatatctg cgccgttagc
     2941 gatcagaatg ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg catctttgtg
     3001 ctcaacaatg ccggtaactt tgccgttttc acgggtgata cgtccataac cggtcggatc
     3061 atccagtttc accgtcagca gaccaatgcc accctgcggt ttagcatcac gcagacgctg
     3121 gagtgtttcg acagagatca gcggcacgtc gccgtagagc attaaaatgt cttcatcatc
     3181 ggcaaagaaa ggtgcggcct gctgcatggc atgacccgta cccagctgct ctgcctgaag
     3241 cacccagttc aggttgtcgt ctttcagcgc ctgttttagc agatcgccac cgtgaccgta
     3301 caccaggtga acgtgcgctg cgcctaattc attcgcagca tcaatgacat gctgaaccat
     3361 cgctttcccg gcaagggtat gcagcacttt cggaagatcg gaatacatgc gcgtgccttt
     3421 acctgcggca aggatcacta cgctcatagc attattcaac atacgcgtcc tgactgtaat
     3481 ttgagaacga atttaaaccg cttcaccttg aaaaaactac atttttttca tcgtgaaatg
     3541 gacagaggat aaattgttca ataacggatt atccccgctg acgaaagcgc cattttcgac
     3601 catcgtcacg ccttttgtct gcagaaaatt aaggcagtac cttcatttaa gcgtcaggtg
     3661 gatgtttttg ctcttatttc gatcaatgaa taaacgggaa aacagggggt tttatatcag
     3721 caggatctat gtgaacgcta ttcaggacgg gtcacacgcg caaaaaaaag ccagcctgtt
     3781 tccagactgg cttttgtgct tttcaagccg gtgttacatc gcttttttgg tcaactcgat
     3841 aacgcgcagc tgcgcgatcg ctttggccag ttccgcagac gcctgagcgt aatctacgtc
     3901 gccgtgagag ctgctaatgt gctcttcagc cttacgtttc gcttccatgg ctcgcgcttc
     3961 gtcgagatcc tggccgcgaa ttgcggtgtc ggccagaacg gtcacgttgc caggctgcac
     4021 ttcaagaatg ccgccagaca gatagataaa ctcttcgtga ccgtgctgtt tcacgatgcg
     4081 aatcatacca ggcttaatgg cggtgagcag cggtgcgtgg ccagggtaga tccccagttc
     4141 accttcgcta cccgttacct ggattttctc gaccagacca gagaacattt gttgctctgc
     4201 gctgacgacg tccaggtggt aagtcattgc catatcaccc tccgattaag gcgttaaagt
     4261 tttttggctt tttccacagc ttcttcgatg gaaccgacca tgtagaacgc ctgctccggc
     4321 aggtgatcgt attcgccttc catgatgcct ttaaagccac ggatggtgtc tttcagggag
     4381 acgtatttac ccggagaacc ggtgaatact tctgccacga agaacggctg ggacaggaag
     4441 cgctggatct tacgagcacg cgctaccacc agtttgtctt cttcagacag ttcatccata
     4501 cccaggatgg cgatgatgtc tttcagttcc tgataacgtt gcaggatgga ctgaacgcca
     4561 cgcgcggtgt cgtagtgttc ctgaccaacc accagcgggt ccagctgacg gctggtggag
     4621 tccagcgggt caacggccgg gtagataccc agagacgcga tctgacggct cagtaccacg
     4681 gttgcgtcaa ggtgcgcaaa ggtggttgcc ggagacgggt cagtcaagtc atccgcaggt
     4741 acgtatactg cctgtacgga ggtgatagaa ccggttttgg tggaggtgat acgttcctgc
     4801 agaacgccca tctcttccgc cagggtcggc tgataaccta ccgctgaagg catacggccc
     4861 agcagtgcgg atacttccgt accggccagg gtgtaacgat agatgttgtc aacgaacagc
     4921 agaacgtcac gaccttcgtc acggaatttc tcagccatgg tcagaccggt cagagctacg
     4981 cgcagacggt ttcccggcgg ctcgttcatc tggccataca ccagggatac tttatcgata
     5041 acgttggagt cggtcatttc gtggtagaag tcgttcccct cacgagtacg ttcacctacg
     5101 cccgcaaaca cagagtaacc ggagtgctcg atcgcgatgt tacgaatgag ctccatcatg
     5161 tttacggttt tacctacacc cgcaccaccg aacagaccaa ctttaccgcc cttagcgaac
     5221 ggacacatca ggtcgataac tttgataccg gtttccagca gttcctgaga gtttgacagc
     5281 tcttcgtagg aaggtgctgc gcggtgaatc gcccaacgct cttcttcacc gatctcgcct
     5341 ttcatgtcga ccggttcacc cagtacgttc atgatacggc ccagagtcgc tttacctacc
     5401 gggacttcga tcgggtgttc gaggtctttt acatccagac cgcgacgcag accgtcggag
     5461 gaacccattg cgatggtacg cacgataccg ccgccgagct gctgctgaac ttccagcacc
     5521 agacgctcat taccattttg cacctcaaga gcatcgtaca cgcgcggtac ggcatcctga
     5581 gggaattcga cgtcaactac ggcgccgatt acctggacaa tctttccagt agccatctta
     5641 aatcctctac gaaataacct gtttaaaccg cggcggcccc cgagacgatc tcggtgagtt
     5701 cctgagtaat gctggcctga cgagctttgt tgtataccaa ctgcagctct ttaatcaggc
     5761 tgccgccatt gtcggtcgcg gctttcatcg ccaccatacg ggcggcctgc tcgctggcca
     5821 ggttttcaac cacgccctga taaacctgag attcgacata acgacgcagc agggtatcca
     5881 gcaacgcctt cggatcgggt tcgtacaggt aatcccagga tttatgtttc agatcatcat
     5941 catctgatgc cggtaacggc agcagctggc tgatggtcgg aacctgagac atggtgttaa
     6001 taaatttgtt gctgacaatg taaagcttgt ccagacggcc ttcgtcgtag gcctgcaaca
     6061 tcacttttac cggaccgatc agttcggaca gggaagggtt atcccccatg ccggtgacct
     6121 gggcaacaac attgccgccc acggagttga agaacgacac gcctttcgag ccgatcattg
     6181 cgaggtcgca ttgaacgcct ttgtcggtcc aggtcttcat ttccgccagc agttttttga
     6241 acaggttaat gttcaaacca ccgcacaaac cacggtcggt cgacaccacc aggtagccca
     6301 cgcgtttaac gtcgcggtct tccaggtaag ggtgcttata ttccagatta ccgtgtgcaa
     6361 ggtgaccaat cactttgcgc atggtttctg cataaggacg gctggccgcc atgcgatcct
     6421 gcgatttacg cattttggaa gcggcgacca tctccatcgc tttagtgatc ttttgcgtgt
     6481 tctggacgct tgcgatctta ctacgtatct cttttgcgcc ggccatgagc ttctcctcaa
     6541 tgccttgcgg cctgccctaa ggcaagccgc cagacgttac caggattggg ttgctttgaa
     6601 ggaatcgagg atgcctttca gcttgccttc gatttcgtcg ttgtagccac cggtctggtt
     6661 gatctcttgc atcaacggag cgtgatcacg gtcgacgtaa gccagcagag cggcttcgaa
     6721 gctgccgatt ttcgacagtt caacatccgc caggtaacca cgttctgctg cgaacagaac
     6781 cagagactgc tgcgcaacgg acatcggcgc atactgtttc tgtttcagca gttcggtcac
     6841 tttttgaccg tggtcaagct gcttacgtgt tgcatcgtca aggtcggatg caaactgaga
     6901 gaacgctgcc agttcacgat actgtgccag agcggtacgg ataccaccgg acagtttttt
     6961 catgatcttg gtctgtgctg caccaccaac acgggatacg gaaatacccg ggttaaccgc
     7021 aggacgaata ccggcgttga acaggttggt ttccaggaag atctgaccat cggtaatgga
     7081 gattacgttg gtcggaacga acgcagaaac gtcacccgct tgagtttcga taatcggcag
     7141 cgcggtcagt gaaccggttt tccctttcac ttcacctttg gtgaaagctt caacgtattc
     7201 ggcgttaaca cgcgcagcac gctccagcag acgagagtgg aggtagaata cgtcgcccgg
     7261 gaatgcttca cgtcctggcg gacgacggag cagcagcgag atctgacggt aagcaacagc
     7321 ctgtttagac agatcatcgt aaatgatcag cgcatcttca ccgcggtcac ggaagtattc
     7381 gcccattgcg caaccggcat acggtgccag gtattgcagt gcagcggatt cagacgcggt
     7441 tgctaccaca acgatggtgt tagccagtgc gccgtgctct tccagtttac gtaccacgtt
     7501 agaaatggtg gacgctttct ggccgatagc gacatagata catttgatac cggaatcgcg
     7561 ctggttgatg atggcatcga tagccagtgc ggttttaccg gtctgacggt caccgatgat
     7621 caattcacgc tgaccacgac cgattgggat catggagtca acggctttat aaccggtctg
     7681 taccggctga tctacggact gacgttcgat aacgcccgga gcgattgctt ctacagcaga
     7741 gaagccgtcg tgatccagcg gacctttacc gtcgattggt gcacccagag tgttaaccac
     7801 acggcccagc aggccacggc caaccggaac ttccaggata cggccagtac acttaacttt
     7861 catgccttcg gcaaggtcag catacggacc cataacaacc gcaccaacag agtcgcgctc
     7921 gaggttcagt gcgatagcgt aacggttacc cggcagggag atcatttcac cctgcataca
     7981 atcggccagg ccgtgaatgc ggataacacc gtcacttaca gaaacaatag taccttcgtt
     8041 gtgagcttca ctcacaacat tgaactgagc aatgcgctgc ttgatcagtt cgctgatttc
     8101 ggtggaattc agttgcatgc tccagtcccc ttaagactgc aagacgtctg caaggcgctc
     8161 aagacgaccg cgtacgctgc catcaatgac catatcaccc gctcggatga taacgcctgc
     8221 cattacagac ttatcgattt tgcaattcag cttaactttg cgtgacagac gtttttccat
     8281 cgcagcagaa attttcgcga gctgttgttc actcagtgcg gcagcggaaa tgacgtctac
     8341 ctcagcggta gcctcactca cggcacgaag gtgaataaac tgctccaaaa catccgggag
     8401 cgcgttaaga cgaccatttt cagccattac ccgaataagg ttctgaccgt tttcgtccag
     8461 ttgctcacca caaactgcga taaacgactc ggcaagcgtt tctggcgcaa gcgcgccaga
     8521 gagaagctct gccatttgtt cgtttttggt tacctcggcg gcaaacgcca gcatgtcctg
     8581 ccagcgttct acactttggt gttcgacggc aaagtcaaaa gctgctttgg cgtaggggcg
     8641 agctaccgta ataaattcag acatcagccc ctccctcctt acagttcagc gacaagttta
     8701 tccacgatgt cgctgttagc agcttcatcc acggaacgtt cgatgatctt ctcggcgcca
     8761 gcaacagcca ggatagcaac ttgcttacgc agctcttcac gggcacgttt acgctcggct
     8821 tcaatttccg cctgcgcctg ggccacgatt ttagtacgtt cctgttctgc ctcagctttc
     8881 gcttcgtcca gaatctgcga gcggcgtttg ttcgcctgct cgatgattac ctgggcttcc
     8941 gctttcgctt ttttcagctg gtcggtcgcg ctggcctttg caaggtcaag gtccttatgt
     9001 gctcgttctg cggaagcaag gccgtcagca atttcttttt gacgtttttc gatggctgcc
     9061 attaatggcg gccatacgta cttcatgcag aacagaacga acaggacaaa cgcgatggcc
     9121 tggccgagga ttgttgcgtt aagattcaca gcacaatgcc tctatttagt taacgttctg
     9181 atattgctct ttaaataaaa gcaacgctta ctacgcgaca gcgaacatca cgtacagacc
     9241 cagacctaca gcgatcatcg ggatagcatc caccagaccc ataacgataa agaactgagt
     9301 acgcagcaga ggaatcagat caggttgacg cgctgcgcct tccaggaatt tacccccgag
     9361 gatgccgata ccgatcgcag caccgattgc cgccagaccc atcatcacag cggcagccat
     9421 gtacagcaga tccatattca ggttttccat gacagtctcc agtttgtttc agttaaaacg
     9481 tagtagtgtt ggtaaattaa tgttcttcag acgccatcga cagatagacg atcgtcagaa
     9541 ccatgaagat gaaggcttgc agcgtaatga tcaggatgtg gaaaatggcc cacggcacat
     9601 tcaggatcca ctgtgaccac cacggcaaca gaccagcaat cagaatgaaa atcagctcac
     9661 cggcatacat gttaccgaac agtcgcaaac cgagtgaaac tggtttggac agcaggctta
     9721 ccccttcaag gattaagttg acaggaatga acgcccagtg attgaacggc tgcagcgtca
     9781 actctttcgt gaagccgccg atgcctttca ttttgatgct gtagaacaga atcaggataa
     9841 atacgcccag tgccatagac agcgttacgt tcacgtccgc agacggaacc acacgcagtg
     9901 caggcagacc cagtacatgt tcagcaatgt acggcagcag gtcgataggc agtaaatcca
     9961 tcaggttcat caggaatacc cagacgaaga tcgtcagggc cagcggagca atcagcttgc
    10021 ttttgccatg gtacatgtct ttcacgctac cattaacaaa gccgatcacc agctcaatcg
    10081 cggtctgaaa cttacctggc acaccgctgg tcgccttttt ggctacgcta cggaataaaa
    10141 ccaggaacaa cagacccagc accaccgaga agaacatgga gtcaatattg attgtccaga
    10201 aggtggctgg ggggttttgt ggatccacca gcgagaatgt acgcaggtcc agctgaaggt
    10261 tattcaggtg gtgtcctatg taatcctgcg gcgtcatatt ttctgaagcc atgatgcctt
    10321 ttaccctttg ttgttaatta cagccggtgc cagtatctga accaccagca ccaaaaccca
    10381 cgtaacgatc agcggcaaga ataccgcctt taaaaccgcc aacgccacca ccagtaacac
    10441 caacatcgcc agaactttga aagcttcacc aaatgcgaat gtccaggcca cccggccttt
    10501 cgctggtgta tgcgcctggt gacgccaggc aaatatcata aacaaaacgt taggcagaaa
    10561 gactgccagg cccccgctta ttgcagagac gccccagaag gggtctttga ggctgaacag
    10621 caatccactt gctatcacca ccagtaactg aacgagcaga agcttccgag caacgtttcg
    10681 actcacgagc gacacagaca tcacgttttt cactcctgct cccttcgagg tatgccgcgt
    10741 gtcgtataaa actttcttta aggcttagag tcaagcatca aaaagcggtc aaattatacg
    10801 gtgcgccccc gtgatttcaa acaataagta gccaaaaggt gaataaatgt ttaaatattt
    10861 ttccagtgca tacaattgcg acttttctac taaccctgtt cgatcatgaa aaactgtaaa
    10921 taacgcgtaa acactggtga taaagcgtgc ttcagatcac atattgcgca tgttcgcgca
    10981 cagcatattt atttacttgg caaatgatgc ctttgcaagt ttatgatatt tcagtctaaa
    11041 a
//
LOCUS       DBFIFM010000152        11037 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.152, whole genome shotgun sequence.
ACCESSION   DBFIFM010000152 DBFIFM010000000
VERSION     DBFIFM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.152"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            137..898
                     /gene="adiY"
                     /locus_tag="N7C74_003495"
     CDS             137..898
                     /gene="adiY"
                     /locus_tag="N7C74_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HGU2433298.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            1035..2372
                     /gene="adiC"
                     /locus_tag="N7C74_003496"
     CDS             1035..2372
                     /gene="adiC"
                     /locus_tag="N7C74_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HGU2433299.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2476..4119
                     /gene="eptA"
                     /locus_tag="N7C74_003497"
     CDS             2476..4119
                     /gene="eptA"
                     /locus_tag="N7C74_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HGU2433300.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4116..4784
                     /gene="basR"
                     /locus_tag="N7C74_003498"
     CDS             4116..4784
                     /gene="basR"
                     /locus_tag="N7C74_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HGU2433301.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4785..5885
                     /gene="pmrB"
                     /locus_tag="N7C74_003499"
     CDS             4785..5885
                     /gene="pmrB"
                     /locus_tag="N7C74_003499"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HGU2433302.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5861..5950)
                     /gene="pmrR"
                     /locus_tag="N7C74_003500"
     CDS             complement(5861..5950)
                     /gene="pmrR"
                     /locus_tag="N7C74_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HGU2433303.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(6062..7564)
                     /gene="proP"
                     /locus_tag="N7C74_003501"
     CDS             complement(6062..7564)
                     /gene="proP"
                     /locus_tag="N7C74_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HGU2433304.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7828..8706)
                     /gene="rdcB"
                     /locus_tag="N7C74_003502"
     CDS             complement(7828..8706)
                     /gene="rdcB"
                     /locus_tag="N7C74_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HGU2433305.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLV"
     gene            complement(8703..10931)
                     /gene="crfC"
                     /locus_tag="N7C74_003503"
     CDS             complement(8703..10931)
                     /gene="crfC"
                     /locus_tag="N7C74_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HGU2433306.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQMLLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQIEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALKVQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2768 a         2873 c         2703 g         2693 t
ORIGIN      
        1 tataaaaatt tgtaatatta gtaaaactcg ttatttttat gcatgtttat attcatcata
       61 caattatata accatttccc ggtatcgctt tgctttagcg agaactggtc ttttattcgc
      121 actcaggagt acatgtatga ggatttgcag cgaccaacct tgtattgttt tattgactga
      181 aaaagatgtc tggataaggg ttaatgggaa agaacctatt agccttaaag ctaaccatat
      241 ggcgttatta aattgtgaaa ataatattat cgacgtctcc tctcttaaca acactttggt
      301 tgctcatatt agtcacgaca tcatcaaaga ttacctccgg tttctgaata aagatctctc
      361 gcaaatacca gtatggcaac gtagcgctac gcccattctc accctgccat gcctgacacc
      421 agacgtcttt cgcgttgccg cgcaacacag catgatgccc gcagaaactg agtcagaaaa
      481 ggaacgaaca cgcgcattat tattcacggt gctatcccgt tttctcgaca gtaaaaaatt
      541 cctttcatta atgatgtata tgttacgtaa ttgtgtaagt gacagcgttt atcaaattat
      601 tgaaagcgat attcacaaag actggaatct tagtatggta gccagttgtt tatgtcttag
      661 cccaagtctg ttaaagaaaa agctgaaaag cgaaaacacc agttatagcc aaataatcac
      721 cacctgccgc atgcgttatg ccgtaaatga attaatgatg gacggtaaaa atatctctca
      781 ggtatcacag tcctgcggct acaacagtac gtcgtacttt atttctgtct ttaaagactt
      841 ctacggtatg acaccgctgc attatgttag tcagcacaga gaacgcactg tcgcctgatt
      901 tttaacctta acgaagagct atattaataa cggcatcagc gataacccgg tcgataataa
      961 ttcaactatc gaatgcaggc gtatgatatg acgtaattta ttgtcacgaa gctcgccttc
     1021 gcaggagttt aattatgtct tcggatgctg atgctcacaa agtgggctta atccccgtca
     1081 ccctgatggt gtcggggaat attatggggt caggtgtttt tctgttacct gcaaacctgg
     1141 cctccaccgg cgggattgcc atttatggat ggttggtgac gattatcggt gcgctggggc
     1201 tctcgatggt atacgccaaa atgtcgttcc tcgacccaag tcctggtggt tcttacgctt
     1261 acgcccgccg ctgctttggc ccgtttctcg gttatcaaac caacgtcctc tactggctgg
     1321 cctgctggat cggcaatatc gccatggtgg tcattggcgt aggatattta agttacttct
     1381 tcccgattct gaaagatcca ttggtattaa ccatcacctg cgtcgtggtg ctgtggatct
     1441 tcgtcctgct gaacattgtc ggtccgaaaa tgatcacccg tgtgcaggcc gttgccaccg
     1501 tgctggcgct gatccccatc gtcgggattg ccgtatttgg ctggttctgg tttcgtggtg
     1561 aaacctatat ggcggcatgg aacgtcagcg gcctgggcac cttcggtgca attcaaagta
     1621 cccttaacgt tacgctgtgg tcgttcatcg gtgtggaaag tgcctccgtt gccgcaggtg
     1681 tggtgaaaaa cccgaaacgc aacgtcccta tagccaccat tggtggggta ttgattgccg
     1741 ccgtttgcta tgtgctctcc accaccgcga ttatggggat gatccctaat gccgcactgc
     1801 gcgtttctgc ctcgccattc ggtgatgccg cacggatggc gttgggtgac accgccgggg
     1861 ccatcgtttc cttctgcgca gctgcgggtt gcttaggttc actgggcggc tggacattgc
     1921 tggcgggtca aacggcgaaa gcggctgccg atgacggact gttcccaccg atttttgccc
     1981 gtgtaaataa agcgggtacg ccagtggcgg ggctgattat cgtcggtatt ttgatgacca
     2041 tcttccagct cagcagcatt tcaccaaacg cgaccaaaga attcggtctg gtttcttccg
     2101 tgtcggtcat ctttacactg gtgccatatc tttacacctg tgcggcgtta ctgctgctcg
     2161 gacacggtca ctttggtaaa gcacgcccgg catatctggc ggttactacc attgccttcc
     2221 tctactgcat ctgggccgtg gtggggtccg gggcgaaaga ggttatgtgg tcatttgtca
     2281 ccctgatggt catcaccgcc atgtatgcgc tgaattacaa ccggctgcat aaaaacccgt
     2341 atcccttaga tgcaccaata agcaaagatt aattcccctt aatccagcaa agataaaagc
     2401 caaccttaag aacttaaggt tggcttaatt ttgctttgcg agcatatgcg cactttgttc
     2461 gatggaaaca ccgtgatgtt gaagcgccta ctaaaaagac cctctttgaa tttactcgcc
     2521 tggctattgt tggccgcttt ttatatctct atctgcctga atattgcctt ttttaaacag
     2581 gtgttgcagg cgctgccgct ggactcgctg cataacgtac tggttttctt gtcgatgccg
     2641 gtcgtagcct tcagcgtgat taatattgtc ctgacactaa gctctttctt atggcttaat
     2701 cgaccgctgg cctgcctgtt tattctggtt ggcgcggctg cacaatattt cataatgact
     2761 tacggcatcg tcatcgaccg ctcgatgatt gccaatatta ttgataccac tccggcagaa
     2821 agctatgcgc tgatgacacc gcaaatgtta ttaacgctgg gattcagcgg cgtgcttgct
     2881 gcgctgattg cctgctggat taaaatcaaa cctgccacct cgcgtctgcg cagtgttctt
     2941 ttccgtggag ccaatattct ggtttctgta ctgctgattt tgctggtcgc cgcactattt
     3001 tataaagact acgcctcgtt gttccgcaac aacaaagagc tggtgaagtc cttaagcccc
     3061 tctaacagca ttgttgccag ctggtcatgg tactcccatc agcgactggc aaatctgccg
     3121 ctggtgcgaa ttggtgaaga cgcgcaccgc aacccattaa tgcagaacga aaaacgtaaa
     3181 aatttgacca tcctgattgt cggcgaaacc tcgcgggcgg agaacttctc cctcaacggc
     3241 tacccgcgtg aaactaaccc gcggttggcg aaagataacg tggtctattt ccctaatacc
     3301 gcatcttgcg gcacggcaac ggcagtttca gtaccgtgca tgttctcgga tatgccgcga
     3361 gagcactaca aagaagagct ggcacagcac caggaaggcg tgctggatat cattcagcga
     3421 gcgggcatca acgtgctgtg gaatgacaac gatggcggct gtaaaggtgc ctgcgaccgc
     3481 gtgcctcacc agaacgtcac cgcgctgaat ctgcctgatc agtgcatcaa cggcgaatgc
     3541 tatgacgaag tgctgttcca cgggctggaa gagtacatca ataacctgca gggtgatggc
     3601 gtgattgtct tacacaccat cggcagccac ggtccgacct attacaaccg ctatccgccg
     3661 cagttcagga aatttacccc aacctgcgac accaatgaga tccagacctg taccaaagag
     3721 caactggtga acacttacga caacacgctg gtttacgtcg actatattgt tgataaagcg
     3781 attaatctgc tgaaagaaca tcaggataaa tttaccacca gcctggttta tctttctgac
     3841 cacggtgaat cgttaggtga aaatggcatc tatctgcacg gtctgcctta tgccatcgcc
     3901 ccggatagcc aaaaacaggt gccgatgctg ctgtggctgt cggaggatta tcaaaaacgg
     3961 tatcaggttg accagaactg cctgcaaaaa caggcgcaaa cgcaacacta ttcacaagac
     4021 aatttattct ccacgctatt gggattaact ggcgttgaga cgaagtatta ccaggctgcg
     4081 gatgatattc tgcaaacttg caggagagtg agtgaatgaa aattctgatt gttgaagacg
     4141 atacgctgtt attgcaggga ctgattctgg cggcgcaaac cgaaggctac gcgtgcgatg
     4201 gcgtgacaac cgcgcggatg gcggaacaaa gccttgaggc aggtcattac agcctggtgg
     4261 tactggattt agggttaccc gacgaagatg gactgcattt tctcgcccgt atccggcaga
     4321 aaaaatatac cctgccggta ctgatcctca ccgctcgcga tacgctgacc gacaaaatcg
     4381 ccgggctgga tgtcggtgcc gacgactatc tggtgaagcc ttttgcgctg gaagagttac
     4441 atgcccgtat ccgcgccctg ctacgacgcc ataataatca gggcgaaagt gagctgattg
     4501 ttggcaatct gacgctgaac atgggtcgcc gtcaggtatg gatgggcggt gaagagttga
     4561 ttctgacgcc caaagaatat gctctgctgt cacggttaat gctcaaagca ggcagtccgg
     4621 tgcatcggga aattctctac aacgacatct ataactggga caatgaaccc tcgaccaaca
     4681 ccctggaagt gcatatccac aatctgcgcg acaaagtggg caaagcccgt atccgcaccg
     4741 tgcgcggctt tggctatatg cttgtcgcga atgaggaaaa ctaattgaat ctgatgcgtt
     4801 ttctgcgccg accaatatcg ctgcgccaac ggctgatatt gaccatcggg gccattttgt
     4861 tggtgtttga gctgatcagc gtcttctggc tatggcatga aagtaccgag cagattcagc
     4921 tgtttgagca ggcgctacgc gacaatcgca acaacgatcg ccacatcatg cgtgagatcc
     4981 gcgaggcggt cgccagcctg attgtccccg gcgtctttat ggtcagcctg acgctattta
     5041 tctgctatca ggcggtacgc cgcatcaccc gcccgctggc ggagctgcaa aaagagctgg
     5101 aagcgcgcac cgccgacaac ttaacgccca ttgccattca cagcgccacc ctcgaaatcg
     5161 aagcggtggt ttcggcgttg aacgatctgg tcagtcgcct gaccagcacg ctggataacg
     5221 aaaggttatt taccgctgac gtcgcgcacg aactgcgaac gccactggcg ggggtgcgtt
     5281 tgcatctgga actgctggcg aaaacgcatc acattgatgt agcaccgtta gtggcacggc
     5341 ttgatcagat gatggagagc gtctcccagc tgctgcaact ggcgcgtgcc ggacagtcat
     5401 tttcttccgg taattatcaa catgtaaaac tgctggaaga tgtcattctc ccctcgtatg
     5461 acgaactcag taccatgctc gaccagcgac agcaaaccct gctattgcca gagagcgccg
     5521 ccgacatcac cgttcagggt gatgcaaccc tgctgcggat gttattgcga aacctggtag
     5581 aaaacgccca tcgttacagc ccgcaaggca gcaacattat gattaagctg caagaagatg
     5641 gcggagcggt catggccgtt gaagatgaag gaccgggtat tgatgagagt aaatgcgggg
     5701 agttgagtaa agcgtttgta cgtatggaca gccgttatgg cgggattggt ctggggttaa
     5761 gtattgtcag ccgcattaca cagttgcatc acgggcagtt tttcctgcaa aaccggcaag
     5821 agacttccgg cacgcgggcc tgggtacggc tgaagaaaga tcagaacgtg gcaaaccaga
     5881 tataaagaaa gctgctgacc accagcacgc tgaacacggt agttaaactt tcataaacac
     5941 ggtttttcat tgcactctcc tccgttaacc tggaggagag tatgcgcgtc agagattaag
     6001 tgaaccttaa gagttcaatt ggcctgataa gacagcgtta catcaggcca tccgtttcag
     6061 cttattcatc aattcgcgga tgttgctgca ccaggcgggt acgtttcgcc tgcaaatcgg
     6121 caatctcgtg gtcaatatca tcgattttct gctcgatatt atcgtaatgc tcgacgagaa
     6181 tttccttcgc ttcctgtata tctgacgccg ccggtgttgc acctttcaac ggacgatttg
     6241 ccgtctcttt catggttacg ccggtgatta aaccaaccac cgccactacc atcaggtaat
     6301 aggctggcat catcagattc tgcgagcttt cgaccagcca ggccgccagt gttggcgtca
     6361 gaccggcaac cagcaccgaa atattaaatg ccgccgccag cgcgctgtaa cggatatgcg
     6421 tcgggaacat cgctggcaag gtagaagcca taacgcccgt aaagcagtta aggatcaccg
     6481 ccagcatcag taacccggca aaaatcaggc cgatgacgtt actgttaatc agaataaacg
     6541 ccgggatcgc caacacaaac agtgcaacac taccaagtag cacaaacgga cgacggccaa
     6601 aacggtcact cagcaagccc atcaccggct ggacaaacag cataccgatc ataatggcga
     6661 taataatcag caccccgtgg tcttcggagt aatgcaggtt atgcgacaaa taactcggca
     6721 tataggtcag caacatgtag taagtcacgt tggtggcaat taccagacca atacatgtca
     6781 acaggctgcg ccagtattta gtggcaatct ctttaaacga gactttcggg ccatcctgca
     6841 aaccttcgcg gtcgccctgt tccagtttat caacatgctg ctggaacgcc ggagtttctt
     6901 ccagcgcatg gcgcaggtaa agcccgataa tccctaacgg cagagcaata aagaacggaa
     6961 tacgccagcc ccagtcgagg aagttcgctt cgccgacaat ggtcgaaatt aacaccacca
     7021 cgcccgcacc cagcacaaac ccggcaatag aaccgaagtc cagccagctg cccataaagc
     7081 cacgtttacg gtcaggggag tattccgcaa caaatatcga cgccccggta tattcaccgc
     7141 cgaccgagaa accttgtgcc atcttacaga tcaacagcag aatcggtgcc caaataccaa
     7201 tcgtgtcgta ggacggtatt aagccaatac agaacgtact gatcgacata atcacaatag
     7261 tgatagcgag gatcttctgg cgaccatatt tatcgcccaa cataccaaag aagagtccgc
     7321 caagcggtcg aatcagaaag ggaacggaga aagtggcaag tgcagcaacc atctgcacgc
     7381 tggggtcagc ccccgggaaa aaaactttac ctaatgcgta agcaacaaaa ccataaacac
     7441 caaaatcaaa ccattccatt gcattaccca gtgatgctgc ggtaatggct ttacgcagtt
     7501 taccgtcatc aataatggtg acatcacgaa gggtaatcgg ttttactttt ttccttttca
     7561 gcatagcttt cctcgcagag ttggcccatt tccgcaaacg ccagaaaaat cagcgcgacg
     7621 gccgcaaaag gggagcggga attgcaggat gcaatctctg taacaagcgt agcgggtttg
     7681 cttacaccct ccggttaagg aatgtacaat caaaccagtt gacgcctgaa ttgggcagtt
     7741 aatgaccttt ttaccctacc agcgtttaaa tttgtgatca acttcacaca tggatttcgt
     7801 ttaggactca ttgatgtaac tgattattta caccaaattt acagagaaga tttcctcgag
     7861 cattgccccc gccagacgct gcgctgaaca gagtcgtggc atccccagcg cgacagtttg
     7921 ccagcgttgg gtcactgacc agcaaacggg ttgtttatct gccgtggtcc actttcccag
     7981 ccaacttaaa aaatcagcat gatccccgcc cgtcggaacg gtcgaagagg caaaccactg
     8041 atggtaatag tgacgcgtgg tcggggccgc attgatactc tctgccagat gttgtgtcgc
     8101 tttcacgcgc aaatcaccca gcaacaacgc caccgcttgc gggcttccca gacgacaggc
     8161 atcgccaaat gccccccaac gcaactcttc gagaacgata aaacagcgcc caggcaacga
     8221 ccaggcgtta tacgccccag cacgccagcg ggtaaaaatt tgctcgctct gttcacccgc
     8281 ctgcaccagt aggcctcgtt gcgttaacgc cttttccttc cagcgacaac gggctgaaaa
     8341 ccactcatac aaggcggcaa tttgctgggg gagctccggg aaagcagagt gctggcggtg
     8401 atccattttt tgcagcagcg ccagatgctg cgcgatctgc gtcagtgcca gatgaccggg
     8461 atccagcatc gacgccagtt tttccaccaa tgtcagacgc agactaagcg gcgcatttaa
     8521 catcccgccg ttcgtccatg tctgaagctg cacgcgcgca agaagttcct gcatcagtcg
     8581 ttcacgaaac tgctgctgat gggcttgtgg aaggcgggca tcatcgccct gcgccagatc
     8641 cactaaaaag cggggataaa ccgactccag cacgcgaccg gggccgtcca gtaacgtctt
     8701 ggtcaatatc gttctgccgt gaaaagtgtt tgaatatcat cgcgtaacag ctgagcgtcg
     8761 gtgtaaatcc agccgtgagt catcacagtc tgctgcaatt gctgttgcat cagcctgacc
     8821 accgattcat tttgttgacg cagagccaga ctttcgcgta aacgcgtctg taattccgtc
     8881 aaacatgaag cgaactcagc gaaaaaagta ttcatgcctg ccgtaacaga ttcatcgacc
     8941 tgctctgcca gaactttagc catttgttgg caataaagat cgacttctgc gcttaatgct
     9001 cgttgcaaca cactgtaatc aaccgtttct gtcggggatt tctcatttcc ccgtccccag
     9061 tcaggctgat tcaaccagcg cgaaaaagtc tcacgcacaa cgcctaatcg cgtgctctgc
     9121 tcgtccgttg cttcctggcg cgaaatgact tcactgaaca gctggcgagt gttgaagtgg
     9181 ggaactacgc cgtgaaaaac aggaaaatga aacccaggac gaaaccctga ctcgctcaat
     9241 tccattttga cttgttgctc aatggggcga ataacatcgg ttaacacccg gcaaagggtg
     9301 gattcaagct cggcaaaacg cagcgtaaag tcgcgactga tggtgttctg cgccgtctgt
     9361 aacagtgtct cacagcgggt acgaatctcg cttaacggct ccgaatcatc ctgaaacaag
     9421 gcggctaact gcaccttcag cgcatcttgc tgttgacgca gaaagtggtt ggcggaggtc
     9481 agggccagct cgatttcatg tttaatctcg ccgctcacct gcgcctgatt gagttgcaat
     9541 agctgcaaac tttcttcgat ctggtggata ttttgccgca attgttcaca agcgacgttt
     9601 aacccgtgcg cacgaaaatc caggtattcc cgcgcctgct gcgcgtaatt caacagttta
     9661 tgcgcggcag atcgcagagc atacaacgag gcgttagcgt aagcggcatg aagcaacatc
     9721 tgaattggct gggcgaacag cgaatcttcc cacaactgat cggcagcatg acgaatatgt
     9781 tcgaggtccg ccagatcggc atgacgccag cgcctgccga gcgcggcatg ggcaaaatct
     9841 tccacccagc gttgttgctc tggcggtggt aacttaccgt tgttggctaa ctcatggcgc
     9901 gcccgatttg ccaggtagcc ccacatcgac gacaccggaa atatctgctg tggcgtaata
     9961 cagcctttca tcagcgtccc ggaaatcagt gcccgcacct ggtcggcgtc gtcactgtta
    10021 cgatcctgtt gatcgaactt attgaccagc acatacagcg gcaccgattg ccccaccgcc
    10081 aaaatcgcct cacggacctc ttcatcggag atcgatttca gttgcgtata atccagcacc
    10141 gccagtaccg ccgaggcgcg tgccagctgc tggttaagca ttttttgcag atgcggttgc
    10201 ccggcttcat ttggcccggg ggtatccagt aacgtcaatt gaccgggata actttccagc
    10261 cccgccagat ggacaaactc cacttcaatc acgggaatat gctcaatggc ggcgtaagca
    10321 gaaaaaggaa aatcgacgtc cagcgccttc gccagtcgca ccaaatcatt caaacttttc
    10381 agacaatgaa aaataggctg ggcacccaga taatattttt cgaaagcgac gccattttcg
    10441 atccgctgca taagcgcacg catatcttta tctatttcca gcacatcggt cagatgctta
    10501 atatcgcaat cacgcaggcg ctgttgtaat ttttgaatta aacaatcgat tggcgcgaca
    10561 tgtgaaaaat gcagtaccgg ttccttttgg ccgggcgtat ggcgaataag cgtcggcagc
    10621 gcagtcattg ggcgattgcg attaggcagt acctccgtac caacaatggc attaatggtg
    10681 gttgatttcc ctgctttcat ggtaccgaca atcgccagca ccatttccag tcgggaaatt
    10741 ttacgcaact cattattcag catcgcgtga cgctcggcga tattaggctg actccagggt
    10801 aaagccagtt gtggcgcatc gtctccgggt acagagagag gcattttttc cagtaactgc
    10861 aactgttggc gagaaagctg taacagccgt tcagcctcct gacttaactc atacagggtc
    10921 tgtgtgtaca tagaaaattc ttccttaaag caaattttat tacttttatt tagccagatt
    10981 gtttttgagt tctgttttcg gcttttataa ttactgcaag aaataatttt atattta
//
LOCUS       DBFIFM010000153        10971 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.153, whole genome shotgun sequence.
ACCESSION   DBFIFM010000153 DBFIFM010000000
VERSION     DBFIFM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.153"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..622
                     /locus_tag="N7C74_003504"
     CDS             <1..622
                     /locus_tag="N7C74_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU2433307.1"
                     /translation="VIELAVNPTFSTHWLIPNLHEFTKLHPDIIVNIHSLANNGDFLN
                     REYDAVIMRENFCAPWAEVEYLFEEEILPVCSGSLLAMSDQKLSVAELLTELPLLHQS
                     TRITGWEEWFALSGVSSPLVNNGPRFDLLSMLIAAVRSNLGVALLPRFAIQHDLDSGD
                     MVIPCDVPIRTGNRFIMTWQEEKSDSPHLQQFREWLLAKSVVPQEM"
     gene            complement(656..1258)
                     /gene="cobO"
                     /locus_tag="N7C74_003505"
     CDS             complement(656..1258)
                     /gene="cobO"
                     /locus_tag="N7C74_003505"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HGU2433308.1"
                     /translation="MEARISTERHVQRQQKLKEQVDTRVAAAIEKKGILIVFTGNGKG
                     KSTAAFGTVTRAVGHGKTVGVAQYIKGQWDNGEYNLLQPLGVEFHIMGTGFTWETQNR
                     QADIDAAKEVWSESKRMLADKRYDLVVLDELTYMLAYHYLDTEEVIASLQNRPAQQSV
                     IVTGRGCHSQILKMADTVSEIRPVKHAFDNGIQAQPGIDW"
     gene            complement(1268..2920)
                     /locus_tag="N7C74_003506"
     CDS             complement(1268..2920)
                     /locus_tag="N7C74_003506"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGU2433309.1"
                     /translation="MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPE
                     ALTLLARQAFYEASFFLRSAHLQQVASILNDQQASSNDKYVALQLLRNAEVSAKGVLP
                     NCQDTGTATIVASKGQQIWTGGNDAEALSKGIYSTFQENNLRFSQNAPLDMYTEVNTQ
                     TNLPAQIDISAVAGDEYHFLCVNKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSL
                     GTAACPPYHIAFVVGGLSADQTLKIAKLASTKYYDNLPTSGNEQGQAFRDIELEKVLL
                     EASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADRNIKAKINKHGIWL
                     EKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMQDLARLPVGTRVSLSGPIVVAR
                     DIAHAKIKARLDSGEPMPEYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYIDTF
                     QAAGGSLVMLSKGNRSQQVTDACHKHGGFNLGSIGGAAALLAQEYVKSLRCLEYPELG
                     MEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH"
     gene            complement(3028..4308)
                     /locus_tag="N7C74_003507"
     CDS             complement(3028..4308)
                     /locus_tag="N7C74_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308785.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HGU2433310.1"
                     /translation="MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFT
                     TIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITV
                     NFFMPGTGINFVAHGAETTGVVAAEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
                     FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVM
                     VSAGIFMGEFYFTMLLLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFP
                     GTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITM
                     LLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSN
                     AMGAAMVSRWEGEHFGEGCRGKALKPNESNVALP"
     gene            complement(4469..4786)
                     /locus_tag="N7C74_003508"
     CDS             complement(4469..4786)
                     /locus_tag="N7C74_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HGU2433311.1"
                     /translation="MKQSIVSLAQVIRSKNAGPYELVLDILFKTKENYERVKSSGQLT
                     PELIARLYHVEPDFIHRIVWFDPSNAVKIVMPRDIISGNVGDNDVYGAQQHAPLLNMS
                     FDL"
     gene            complement(4783..6153)
                     /locus_tag="N7C74_003509"
     CDS             complement(4783..6153)
                     /locus_tag="N7C74_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HGU2433312.1"
                     /translation="MARSFKVLSPTAILGYGFPEESFRKAMAESPDLIAVDAGSSDPG
                     PHYLGAGKPFTDRAGVKRDLRYMITAGVQNNIPVVIGTAGGSGAAPHLEWCREIIHEI
                     AREEHLSFSMALIPADVDKAIVHQALDNGKITALDFVPPLTHDAIDESTYIVAQMGIE
                     PFQRALKERAQVVLGGRAYDPACFAALPIMQGFDEGLALHCGKILECAAIAATPGSGS
                     DCAMGIIDDNGFTLKTFNPKRKFTETSAAAHTLYEKSDPYFLPGPGGVLNLKGCTFKA
                     VNDGEVYVSGSKHEETPYALKLEGARRVGFRCLTIAGTRDPIMIAGIDKIIDEVKTSV
                     SRNLSLDDDSIRINFHLYGKNGVMGDHEPMQTAGHELGIVLDVVAPTQEIANSVCSLV
                     RSTMLHYGYENRIATAGNLAFPFSPSDIQGGPVYEFSIYHLIEANDALRFDFHIEQVT
                     PEGVQA"
     gene            complement(6157..7398)
                     /locus_tag="N7C74_003510"
     CDS             complement(6157..7398)
                     /locus_tag="N7C74_003510"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050096 - methylaspartate ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HGU2433313.1"
                     /translation="MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFN
                     AVRQAGECVSVQLILENGAVAVGDCTAVQYSGAGGRDPLFLAEHFIPFLNDHIKPLLV
                     GRDVDAFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
                     PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWL
                     SDRILSKRTSARYHPTLHIDVYGTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPV
                     DAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFTDAASCHMVQIKTPD
                     LGSIHNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMPMLVKPGMGFD
                     EGLDIVFNEMNRTIALLQAKD"
     gene            complement(7398..8843)
                     /locus_tag="N7C74_003511"
     CDS             complement(7398..8843)
                     /locus_tag="N7C74_003511"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HGU2433314.1"
                     /translation="MELRNKKLTHDEFMTERQQVLKTWETGKDVENFEDGVKYQQTIP
                     EHKRFSLALLKADKEGKTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNR
                     YEEAAVGIKKSIEAGTSKLNGLPVVNHGVAACRRLTETLQKPLQIRHGTPDARLLAEI
                     SMASGFTSYEGGGISYNIPYAKRVTLEKSIRDWQYCDRLMGMYEEHGIRINREPFGPL
                     TGTLIPPFISHSIAIIEGLLALEQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQ
                     SYGYTDYELSTVFHQWMGGFPEDESKAFAIISWGAAVAGMSGATKVITKSPHEAWGIP
                     TAAANIQGLKASRQMLNMVNEQKFPPCPAVELEIELIKSEVRAVLNKVFELGNGDIAR
                     GTVLAFEAGVLDVPFAPAACNAGKILPVRDNTGAIRVLEAGAVPLPKDILDLHHDYVA
                     ERARCEGRQPTFQMVVDDINAVSHSKLIGRP"
     gene            complement(8862..10250)
                     /gene="glmL"
                     /locus_tag="N7C74_003512"
     CDS             complement(8862..10250)
                     /gene="glmL"
                     /locus_tag="N7C74_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HGU2433315.1"
                     /translation="MQIVSVDIGSTWTKAALFTREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLNQVLNVDNALPLLNSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQYYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPVAARQAICDIFLKRIVKGKGLDV
                     IVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSACANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAVVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            complement(10250..10699)
                     /gene="glmS"
                     /locus_tag="N7C74_003513"
     CDS             complement(10250..10699)
                     /gene="glmS"
                     /locus_tag="N7C74_003513"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HGU2433316.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFAPSHDLEDVCQLMAHDINQRHDVDTRILEEAI"
BASE COUNT         2581 a         2765 c         2762 g         2863 t
ORIGIN      
        1 ggttattgaa cttgccgtta atcctacttt tagtacccac tggttaatcc caaatttaca
       61 tgaattcaca aagttacatc ccgatattat tgttaatatt cattctttag ctaataacgg
      121 cgattttctt aatcgggagt acgatgctgt cattatgcgc gaaaattttt gcgcgccctg
      181 ggcggaagta gaatatctct ttgaagagga gattttgccg gtatgcagcg gcagtttgct
      241 tgcaatgtcc gatcaaaaac tgtcagtggc agaactgctt actgagttac cacttctgca
      301 tcaaagcacc cgtattactg gctgggaaga gtggtttgct ttatctggcg ttagcagtcc
      361 gctggtcaac aacggaccac gtttcgattt gctttcaatg ctgattgccg ccgtgcgctc
      421 taatctcggc gtggcgctgt taccacgttt cgctattcaa catgatttgg atagtggcga
      481 tatggtgatt ccctgtgatg tgcctattcg taccggtaat cgttttatta tgacgtggca
      541 ggaagagaag tctgactcac cgcacttgca gcaatttcgt gaatggttgc tggcgaaatc
      601 ggtcgtaccg caggagatgt aaaacctgcg cctggcggga tgccaggcgc agttattacc
      661 agtcgatacc cggctgggcc tgaatgccgt tatcaaaagc atgtttgacg gggcggattt
      721 cgctgaccgt atctgccatt tttaatatct gactgtggca accgcgtccg gtgacaatga
      781 cactttgttg tgccgggcgg ttttgcagag aagcgatcac ttcttcggta tccagataat
      841 ggtaagccag catataggtc agctcatcaa gcaccactaa gtcgtaacgc ttgtcagcca
      901 gcatccgttt gctttccgac cagacctctt tcgccgcgtc gatatccgct tgccggttct
      961 gtgtctccca ggtaaaaccg gttcccataa tatgaaactc aacccccagc ggctggagaa
     1021 ggttgtattc gccgttgtcc cattgcccct tgatgtactg cgcaaccccc accgttttgc
     1081 catgacccac cgcgcgcgtc acagtaccaa acgcggcagt ggatttccct ttgccgttgc
     1141 cggtgaaaac aatcaagatc ccttttttct ctatcgccgc cgcgacgcgc gtatcgacct
     1201 gttccttcaa tttctgttgg cgctgcacat gccgttcagt tgaaattcgc gcttccattc
     1261 attttcctta atgtcccgca gggcaactgg cgcagcggtg ttgctgctca aactgactaa
     1321 agaaattatt gcctttatca tctaccagga tgaatgcggg cagattttcc acttccatca
     1381 tccacaccgc ttccataccc agctccgggt attccaggca acgcagactt ttgacatatt
     1441 cttgcgccag taacgcagct gcaccgccaa tacttcccag attgaagccg ccatgtttgt
     1501 gacaggcatc ggtgacctgc tggctgcggt tacctttcga taacatcact agactgccgc
     1561 cagccgcctg gaaggtatcg atataaccgt ccattcgacc gcccgtggtt ggacccagtg
     1621 aaccgcaggc catgttctcc ggtgttttcg ccgggccagc gtaatagaca atatgatgct
     1681 tcaggtactc cggcatcggt tcgccgctat ccagccgcgc ttttattttg gcgtgggcga
     1741 tatcgcgcgc cactacgatg ggaccactta acgacacgcg ggtgcctact ggtaatctgg
     1801 cgagatcctg catcacgtca cgcaacggac ggtttagatc cagttgtaca tgttgcgcgt
     1861 gattttcttc ccgcagtgag gctggaatat attgccccgg attgtgttcg agtttttcca
     1921 gccagatacc gtgtttgtta atttttgctt tgatattgcg atcggcagaa caggagagcg
     1981 ccatagctat cgggcaagag ccaccgtgac gcggcagacg aatgacgcga atatcatgag
     2041 cgaaatattt acccccaaat tgcgcaccaa taccaaattg ctgactggcc tcgagcaaca
     2101 ctttttccag ctcaatatca cggaatgctt gtccctgctc gtttccgctg gtgggcaaat
     2161 tgtcgtaata tttcgttgag gccagcttgg caatttttaa ggtttgatcg gctgaaagcc
     2221 ctccgacgac aaaggcgatg tggtaaggcg ggcaggcagc ggtgcccagc gatttcattt
     2281 tttcgattaa aaatgcggtc agtttttctg gttgcaataa ggatttggtt tcctgataaa
     2341 gcgcggcttt attggcggag ccgccccctt tattaacaca gagaaaatgg tactcatcac
     2401 cagcgacagc gctgatatca atctgcgccg ggagattggt ttgcgtattg acttcggtat
     2461 acatatccag cggcgcattt tgtgaaaagc gcagattatt ttcctggaaa gtactgtaaa
     2521 tgcctttact taaggcttcg gcgtcgttac ctccggtcca tatctgttgc cctttactgg
     2581 caacaatagt tgcggtgccg gtatcctgac agttgggtaa tacccctttt gctgagactt
     2641 cagcattacg taataactgc aacgcaacgt atttatcatt actgctggcc tgctgatcgt
     2701 taagaatgct ggcgacctgt tgcaaatgag cggaacgtaa gaaaaaagag gcttcataaa
     2761 atgcctgccg ggcaagcagc gttaatgctt caggagcgat ttttatcact tcttcgccgt
     2821 ctaattccgt tactgtaata tgttgatcgc taattaatgt atattctgta ccgtctttat
     2881 tttgtagaaa cggttcttgc cagataaatg gtttggacat atttcacttt tcaccttaaa
     2941 cgttagtgtt acttaattgc cgtctaactt catgatccgg cttataaaaa tgcgtagtca
     3001 ttggcttctg attgcttaag atccccctca gggcagagca acgttcgatt cattgggttt
     3061 taatgcttta ccccgacagc cctcgccgaa atgttcgcct tcccaccgac tcaccatcgc
     3121 agcgcccatt gcgttgctca tgacgtttgt cgcggaacgc cccatatcaa ggaacggatc
     3181 aacgcccatc agcaagatca gccccgcttc cggaatattg aactggttga gcgtggcggc
     3241 gataaccacc attgaggcgc gtggtacgcc agccattcct ttcgaggtca acatcaggat
     3301 caacagcatg gtgatttgct caccgatgga taaatggata ttgcaggcct gggcgatgaa
     3361 aactgtggcg aaggagcagt aggccattga tccaacgaga ttaaatgagt agccaatggg
     3421 taagacaaag ctggcaattt tgggggaaac gccaaattgc tccagttttt caagcgttcc
     3481 cggaaaagcc gcttcagaac tggatgtggt aaatgccagc agggcgggtt ccgaaagggc
     3541 gcgggtcagg cgtctgatgc aggggccgac ataaacgatg gccagaccga taagcagtac
     3601 ccaaagtaat aacatggtga aataaaattc acccataaag atcccggcgc tcaccataac
     3661 tgccagtcct cgttcagcaa tcaatgctga aatagcagcg aatacggtca ggggagcgaa
     3721 gagcatgacg tagccagtga gctttaacat ggcatgtgcc agcgaatcta aggcgtgaac
     3781 gatggcgctg cctttctcgc caatcgccgt caggctacag ccgaggaaaa ttgagaacac
     3841 cacgatttgc aaaatttcat tgtgcgccat ggcatcgaca atgctggtgg ggaaagcatg
     3901 cgaaataaat acttttagcg taaagggttc tgccgcgacc actccggtgg tttcggcacc
     3961 gtgtgcaaca aaattaattc ctgtacctgg catgaagaaa tttaccgtta tcaagcctaa
     4021 ggcaattgac agcaatgagg cgcaaataaa taaaaagagt gttttagaaa aaatacgacc
     4081 aagggctttg gcatcgccca ttttagcaat acctaccacc agggtagaga ccactaacgg
     4141 agcgataatc atttttatca gtcgtaagaa aatcgtcgtg aatatcgata tttcttgcgc
     4201 atagagtttt gcggtttccg gtgaagcagt gttattgagc actacgccga taatcattcc
     4261 aagtaccagc gccaaaagaa tcatcgtggt taaacttatt ttcttcattt tttcctccat
     4321 caggtgcacg gcgataaatc agaatcagga gaagtagatg cctgaataga gattcaggca
     4381 tctacctgct attacaacct tacaaatagc tgaagtgttg aactgcaata agtgacggga
     4441 tgtcagttgg ctaaaagtat tcggattttt acaaatcgaa actcatattt aataaaggtg
     4501 catgttgctg tgcaccatag acatcattgt cacctacatt cccggagata atatctcgtg
     4561 gcatgacgat ttttactgcg ttagaaggat caaaccagac aatacgatga ataaagtcag
     4621 gttcaacatg atataagcgt gcaatcagtt ctggggttaa ttgccctgat gatttaactc
     4681 gctcatagtt ttctttcgtt ttaaataaaa tatctaaaac cagttcgtaa ggtccggcat
     4741 ttttcgaacg aatgacttgc gccagtgaga caattgactg tttcatgcct gaactccttc
     4801 tggcgtcacc tgttcaatat ggaaatcaaa acgcagagcg tcgttggctt caatcaggtg
     4861 atatatggaa aattcgtata ccggcccgcc ctggatatca gaaggggaga acgggaacgc
     4921 cagattacct gcggtagcga tgcggttttc atagccgtag tgcagcatgg tagagcgcac
     4981 cagcgagcaa acgctgttgg caatttcctg ggtcggtgca actacatcaa ggacaatccc
     5041 cagctcatgc ccggcagttt gcatcggttc atggtctccc atcaccccgt ttttgccgta
     5101 caggtggaaa ttgatgcgaa tgctgtcatc gtcgagcgac aggttacgcg aaacgctggt
     5161 tttgacttca tcgatgattt tatcgatccc ggcgatcata atcgggtcgc gcgttccggc
     5221 gatggtcaga cagcggaagc ccacccgtcg tgcaccttcc agtttcaggg catacggcgt
     5281 ttcttcatgc ttagaaccgc tgacgtagac ttcgccgtca tttactgctt taaatgtgca
     5341 gcctttcagg ttcaacacgc cgccaggtcc tggcaggaag tagggatcgg atttctcata
     5401 cagtgtgtgt gcagctgctg acgtttcggt aaatttacgc ttcggattaa atgttttcag
     5461 cgtaaagccg ttgtcatcaa tgatgcccat cgcacagtca gagcctgagc cgggcgttgc
     5521 ggcgattgcc gcgcattcaa ggattttccc acaatgcagc gccagacctt catcaaagcc
     5581 ttgcatgatc gggagcgcag caaagcaggc cgggtcgtaa gcgcgcccgc ccagcaccac
     5641 ttgcgcgcgt tctttcagcg cccgctggaa gggttcgatg cccatttgcg cgacgatata
     5701 cgtactttcg tcaatcgcgt cgtgggttaa cggcgggaca aaatccagcg ccgtgatttt
     5761 gccgttatcc agcgcctggt ggacgatggc tttgtcaacg tctgccggga tcaacgccat
     5821 cgagaacgag aggtgctctt cccgggcaat ctcatgaatt atttctcggc accactccag
     5881 gtgtggtgcg gcaccagaac cgccagccgt tccgatcacc accggtatat tattttgcac
     5941 gcctgcggtg atcatatagc gcagatcgcg cttgacccct gcgcgatcgg taaacggttt
     6001 gcctgccccg aggtagtggg ggcctggatc ggaagagcct gcatcaacgg cgatcagatc
     6061 gggcgattct gccatcgctt tgcggaagct ctcttccggg aatccatacc cgaggattgc
     6121 cgttggcgat aagactttaa aagaacgagc catttgttaa tccttagcct gcaacagcgc
     6181 gatagtacga ttcatttcgt tgaagacgat atcgaggcct tcgtcaaagc ccatccctgg
     6241 ttttaccagc ataggcatgg ggcgagcggc aagggcgacg tggacacagg tgcgggcact
     6301 gacatcagtt tcattgcagg tgccgccctg gtacgcttcc atgctgtggc tgttgcagta
     6361 aagaaccgca tcgacgatgt tgtgaatact gcccagatcc ggggttttga tttgcaccat
     6421 gtgacaactg gcagcatcag tgaaatcaac aatatcctgg taggtgttac accattcatc
     6481 ggccacaatt ttcacgccgg aaccgaggcg cgtcagctct ttggtaatcg cggtcagcag
     6541 gcgaatttga tcgggcttgt taccggcatc gaccggccct tcgatgtaga gcggcaggcc
     6601 ttgcgcttct ttttccaggc tggcgatgta ttgcgcacag cgaagcggat ccatatcgaa
     6661 gatcagaccg atagtgccgt atacatcgat gtgcagggta gggtggtagc gtgcgctggt
     6721 gcgcttgctt agaatgcgat ccgacaacca gcggacatat tcgcgcagtt tttcaccttt
     6781 aaagcccagc ttctcttcga cgttattaat cagcgcatgg ggcagcacgt cgatgccttt
     6841 aaggatcatc ttatcgacgg cgatatatcg atcgtcgccg ctctgaccaa ataatggaat
     6901 ggattccgcc acgcgtggca actgccattc atcacagacc acttcagttt tcagacggcc
     6961 ggttgccagc gcggtagcat caagcagcgc ctgtgataat ccgtagcgca cggcggtatg
     7021 cagcaagttg ccgtcaatac gcaatttgtc gaagaaacgg gcattcggca ggaaagcatc
     7081 cacatcgcgg cctaccagta atggcttgat atggtcgttg aggaacggaa taaagtgctc
     7141 tgcgaggaac agtggatcgc gaccgcctgc cccggaatac tgtacggcag tacagtcacc
     7201 tacggcgacc gcgccgtttt ccagaatcaa ctgtaccgat acgcactccc cggcctgacg
     7261 tacggcgtta aacccctgtg ttactggctc cccggtataa aaaaagccgt catgacccgc
     7321 tccgtttttt atcgcctgct ggtcatcgaa atagaatgag gagtagccag cggtgaacag
     7381 agcctgtttt attttcatta tggtcttcct attaatttac tgtgggatac agcattgatg
     7441 tcatctacaa ccatctggaa tgtcggttgg cgtccttcac agcgtgcgcg ttccgcaacg
     7501 taatcgtggt ggaggtcgag aatatctttt ggcaatggaa cggctccggc ttccagtacg
     7561 cgtatcgcgc cggtgttgtc acggaccgga agaattttgc ccgcgttgca ggcggctggc
     7621 gcaaacggca catccagtac gcccgcttca aatgccagca ccgttccgcg cgcaatatca
     7681 ccattaccca gttcgaacac tttattcagt accgcgcgaa cctcgctctt aatcagttca
     7741 atttccagtt caaccgcagg gcacggcggg aatttctgct cattgaccat gttgagcatt
     7801 tggcgggagg ctttcagccc ctgaatgttg gcagcggcag tcgggatacc ccaggcttcg
     7861 tgcggacttt tggtgatgac tttggtggca ccagacatcc ccgcgacggc tgcgccccag
     7921 gagataatag cgaacgcttt cgattcgtct tccggaaagc cgcccatcca ctggtgaaac
     7981 acggtactca attcatagtc ggtatagcca tagctctgaa aatattcgtg tgcaagttca
     8041 cgcagagact ggatcgccgc aacatcctgc gtcaggctgc cgacctggcc ataaccaacg
     8101 gtgatagatt tcacaccctg ttccagcgcc agcaagcctt cgattatcgc gatagagtgc
     8161 gagataaacg gcgggatcag cgtacccgtt agtgggccaa acggttcgcg gttgatgcgg
     8221 atcccgtgtt cttcatacat ccccattaaa cggtcgcagt actgccagtc gcggatcgat
     8281 ttttccagcg ttacgcgttt ggcatacgga atgttgtagg aaatgccacc gccttcataa
     8341 ctggtaaagc cgctggccat tgagatttca gccagcagac gcgcatctgg cgtgccgtgg
     8401 cggatctgta gcggtttttg cagggtttcg gtgaggcgac ggcaagctgc gacaccatgg
     8461 ttaactaccg gtaaaccgtt cagtttagag gttccggctt cgatggattt tttaatccct
     8521 acagccgctt cttcgtaccg attcagacgg gtatatgcat cgatggtgct tggcagcaga
     8581 tcgcactctt cttgtagtgt tttcagcagt tcgatgtgtt cgtccatcag cgccacacct
     8641 gcgcgcggtt ggctaagggt tttaccttcc ttgtctgctt tcagcagcgc aagagagaaa
     8701 cgtttatgct cagggatggt ttgttggtat ttaacgccat cttcaaagtt ttcaacatcc
     8761 ttgccggttt cccaggtttt cagtacctgt tgccgctcgg tcatgaattc gtcatgggtt
     8821 aatttcttat ttcgaagttc cattgattgc agccttaatt gttatagggt taagcattga
     8881 atactggtgc gcgcggcggc cagcggatcg actctggcga cgtttgccag cagtggtaat
     8941 agcccccggg aatcgcggta atattcaaat tcggtcggta agagaatgcg tttaccgtcg
     9001 tcgtttaact cgcggtattt caaccagtga tgcatatcga actgactggc acgcgagagc
     9061 catccgccgg atccgacgac tttacgcacg gttgtcaggt cacgtcccat ctgtaaatcg
     9121 acattgccga cgcaggtgca gacctctttt ttggtgcccg catgtctttc ggtggcataa
     9181 cccacacaca gtccggccag gacggtatcg aaatatttct cttctgcatt tgcgggtaag
     9241 tagtcgggat gagcgactag atgacggagg taatgataaa aagcgtccag ctgtgcgggt
     9301 tgctgagcaa aattcacatt gactaactct ttcgcacttt caccaaccac cactgccgag
     9361 acgcgcattc ccagatcgcc ttccaccgtc cgttttacga agggttccgg tacgccgtgt
     9421 aaaacggtgt cgggcgagag cgtgttggca caggcggaat aaacatcggt ggttgcgccg
     9481 cccatgtcga tcagcatgaa ctctttccag ctgtgatcga cgttactgat tgccttgacc
     9541 agttcaaaga ccgtccatgg tgtcggcatt ggttcttcgc ctgttttatc aacaatgaca
     9601 tccagccctt tccctttaac gatgcgttta aggaatatgt cgcaaatagc ctggcgtgca
     9661 gctacagggt tcggatggtc aagatcgggc agaacgttgt cgacaatggt caggtcttta
     9721 tgcccgagga tctcctgcac ctcgtcctga atatcccggt ttccggcata aataatggcg
     9781 cagtcgagtt tggattctgc cagcacacgg gcgttattca gtccgtagct ttcttctccg
     9841 ccgtcggtgc cgccggtgaa caagagaata tcgggctgcg tttcttccag tgcctggata
     9901 tcacggcggt tcagcttgta ggcgtaatat tgcgcaattt tagcacccgc agagtgagcg
     9961 gttactttcg cggtttccag ggtgatagaa ggcaccaatc ccattgcggc aaccgccagc
    10021 ccacctttgg cagaggagga atacttcaat gcaacctcac cgctgtttaa cagggggagg
    10081 gcattatcta cgtttaacac ctggttcagg ctggagaaaa accccttcgc cagatgatgg
    10141 gtggtggttg gggtcagtac gtggttaacc aacgttaacg cgtccccctc ccgggtaaag
    10201 agggctgctt tggtccacgt cgatccaata tcgacagaga cgatttgcat cagatagcct
    10261 cttcaagaat acgagtatca acgtcatgac gttggttgat atcgtgggcc atcagctggc
    10321 aaacatcttc taagtcatga cttggggcaa agacgcgatc aaatcccatc tctttgaatt
    10381 tggtttccac gtcagcgaag tcatgttttc cgaccaccag gttaccgccc acatagagca
    10441 ggatgtcccc cagtccacgc tcgatgcagc gttcgcgcat gcccagacag tcgatatcgc
    10501 catgcccata gatagaggag acgacaatcg cgtcagcgcc agtttcaatg gcagcatcaa
    10561 tatattcgtc ctggctgacc attacgccca gattaatgac tctgaagtcg tggttgctaa
    10621 aaacgcggtc cagaacttta ttgcctactg catggcagtc cgcgccaata acgccaatca
    10681 caagtgttgc tttcttcatg ggtaatcctt tgtaagacag agagtaaaat aagttacaca
    10741 cgtcgtattt atggccgttc ataacggcac aggtttaata tcacaggagt gtctttatct
    10801 gaccgtaatg ttgcaattga tctattttca aaatgtcttt cgggaaatga ttgtttgaag
    10861 tgtgttttta aatttccatg ttaattcagt gtgttatgtc aactaatgtc atgagtgtta
    10921 aatttttaaa attaacttga tttaatgtat tgagataatt gtgatctact t
//
LOCUS       DBFIFM010000154        10945 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.154, whole genome shotgun sequence.
ACCESSION   DBFIFM010000154 DBFIFM010000000
VERSION     DBFIFM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.154"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..122
                     /locus_tag="N7C74_003514"
     CDS             <1..122
                     /locus_tag="N7C74_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HGU2433317.1"
                     /translation="GFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(242..421)
                     /gene="yoaH"
                     /locus_tag="N7C74_003515"
     CDS             complement(242..421)
                     /gene="yoaH"
                     /locus_tag="N7C74_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HGU2433318.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            495..1856
                     /gene="pabB"
                     /locus_tag="N7C74_003516"
     CDS             495..1856
                     /gene="pabB"
                     /locus_tag="N7C74_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HGU2433319.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRRVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPAPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            1860..2438
                     /gene="nudL"
                     /locus_tag="N7C74_003517"
     CDS             1860..2438
                     /gene="nudL"
                     /locus_tag="N7C74_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HGU2433320.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2622..3986
                     /gene="sdaA"
                     /locus_tag="N7C74_003518"
     CDS             2622..3986
                     /gene="sdaA"
                     /locus_tag="N7C74_003518"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HGU2433321.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     TANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4117..5715
                     /gene="pdeD"
                     /locus_tag="N7C74_003519"
     CDS             4117..5715
                     /gene="pdeD"
                     /locus_tag="N7C74_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU2433322.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRVLIKGSPVLIQWTPAASSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFSFTINVNGPGAPALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5719..7275)
                     /gene="yoaE"
                     /locus_tag="N7C74_003520"
     CDS             complement(5719..7275)
                     /gene="yoaE"
                     /locus_tag="N7C74_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HGU2433323.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7263..7421)
                     /gene="yoaL"
                     /locus_tag="N7C74_003521"
     CDS             complement(7263..7421)
                     /gene="yoaL"
                     /locus_tag="N7C74_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HGU2433324.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            7738..8709
                     /gene="manX"
                     /locus_tag="N7C74_003522"
     CDS             7738..8709
                     /gene="manX"
                     /locus_tag="N7C74_003522"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HGU2433325.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            8772..9572
                     /gene="manY"
                     /locus_tag="N7C74_003523"
     CDS             8772..9572
                     /gene="manY"
                     /locus_tag="N7C74_003523"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HGU2433326.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9585..10436
                     /gene="manZ"
                     /locus_tag="N7C74_003524"
     CDS             9585..10436
                     /gene="manZ"
                     /locus_tag="N7C74_003524"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HGU2433327.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10491..>10945
                     /gene="yobD"
                     /locus_tag="N7C74_003525"
     CDS             10491..>10945
                     /gene="yobD"
                     /locus_tag="N7C74_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HGU2433328.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLERIYKLLVST"
BASE COUNT         2664 a         2784 c         2768 g         2729 t
ORIGIN      
        1 ctgggttcac ggaaaaaatg gttggatggg caaaaaaaat ggagacaggt gaacgctctg
       61 tgattaaaaa tcctgagtac ttttcaacat atatgcagga agaactaaaa gcactggtct
      121 gagttaaatt tatatcagca taaatgggtc aggacgcttt taatcacata taaaaagcgt
      181 cctttttctc acaatcaaat aatcatcagc ttctataacg aaatcgatac ggattagtgt
      241 gttactcgtc ttcatcctca aaacgcgcca cgattcgctc gccggagtgg ttggcacgca
      301 gctcttctgc caccagcgcg attgcctggc cgctgctcat cccttgtgcc atcagttcct
      361 ggatccgctc gacagctttt tgctgctgtt catgggtgag tgaaggtaaa cctgcaaaca
      421 ttgttaactc ctgctaaatt gttggcgcta attatttcat gctacccggc acatagccag
      481 tagagtcagg actgatgaag acgttatctc ccgctgtgat tactttaccc tggcgtcagg
      541 acgccgctga attttatttc tcccgcttaa gccacctgcc gtgggcgatg cttttacact
      601 ccggctatgc cgatcatccg tatagccgct ttgatattgt ggtcgccgat ccgatttgca
      661 ctttaaccac tttaggtaaa gaaaccgttg ttagtgaaag cgaaaaacgc acaacgacca
      721 ctgatgaccc gctacaggtg ctccagcagg tgctggatcg cgcagacatt cgcccaacgc
      781 ataacgaaga tttgccattt cagggcggcg cactggggtt gtttggctac gatctgggcc
      841 gccgttttga gtcactgcca gaaattgcgg aacaagatat cgttctgccg gatatggcag
      901 tgggtatcta cgattgggcg ctcattgtcg accaccagcg tcatacagtt tctttgctga
      961 gtcataatga tgtcaatgcc cgtcgggcct ggctggaaag ccagcaattc tcaccgcagg
     1021 aagatttcac gctcacttcc gactggcaat ccaatatgac ccgcgagcag tacggcgaaa
     1081 aatttcgccg ggtacaggaa tatctgcaca gcggtgattg ctatcaggtg aatctcgccc
     1141 agcgttttca tgcgacctat tctggcgatg aatggcaggc attccttcag cttaatcagg
     1201 ccaaccgcgc gccatttagc gcttttttac gtcttgaaca gggtgcaatt ttaagccttt
     1261 cgccagagcg gtttattctt tgtgataata gtgaaatcca gacccgcccg attaaaggca
     1321 cgctaccacg cctgcccgct cctcaggaag atagcaaaca agcagaaaaa ctggcgaact
     1381 cagcgaaaga tcgtgccgaa aatctgatga ttgtcgattt aatgcgtaat gatatcggtc
     1441 gtgttgccgt agcaggttcg gtaaaagtac cagagctgtt cgtggtggaa cccttccctg
     1501 ccgtgcatca tctggtcagc accataacgg cgcaactacc agaacagtta cacgccagcg
     1561 atctgctgcg cgcagctttt cctggtggct caataaccgg ggctccgaaa gtacgggcta
     1621 tggaaattat cgacgaactg gaaccgcagc gacgtaatgc ctggtgcggc agcattggct
     1681 atttgagctt ttgcggcaac atggatacca gcattactat ccgcacgctg actgccatta
     1741 acggacaaat atactgctct gcgggaggtg gaattgtcgc cgatagccag gaagaagcgg
     1801 aatatcagga aacttttgat aaagttaata agatattacg ccaactggag aagtaagacg
     1861 tggaataccg tagcctgacg cttgatgatt ttttatcgcg ctttcaactt ttgcgcccgc
     1921 aaattaaccg ggaaacccta aatcatcgtc aggctgccgt gttaatcccc atcgtccgtc
     1981 gaccgcaacc ggggttgttg ctgactcagc gttcaattca tctgcgtaaa cacgctggac
     2041 aagtggcatt ccctggaggt gcagtcgatg acacggacgc atcagttatc gccgccgcgc
     2101 tgcgcgaagc tgaagaagag gttgctatac cgccttccgc cgttgaagtt atcggcgtgc
     2161 tgccgcccgt cgatagcgta actggctacc aggtaacccc agtggtcggc attatcccgc
     2221 ccgatctgcc gtatcgcgcc agtgaagatg aagtttccgc tgtgtttgaa atgccgctcg
     2281 cccaggcatt acatctgggt cgttatcacc ctttagatat ctaccgccgc ggcgattcac
     2341 atcgggtatg gctatcctgg tacgaacagt attttgtatg gggaatgacc gcaggcataa
     2401 ttcgtgagct ggcgctgcaa attggtgtga aaccctgact atacttatct ttacatctac
     2461 aaaacactac ttgagacaat catcgcaata ttagttaaat cgcggttttt gattagttta
     2521 attcatgtga atagttaagc cagtcgccgc gttccctctt acactatgcg ctgttattag
     2581 ttcgttactg gaagtccagt caccttgtca ggagtattat cgtgattagt ctattcgaca
     2641 tgtttaaggt ggggattggt ccctcatctt cccataccgt agggcctatg aaggcgggta
     2701 aacagttcgt cgatgatctg gtcgaaaaag gcttactgga tagcgttact cgcgttgccg
     2761 tggacgttta tggttcactg tcgctgacgg gtaaaggcca ccacaccgat atcgccatta
     2821 ttatgggtct tgcaggtaac gaacctgcca ccgtggatat cgacagtatt cccggtttta
     2881 ttcgcgacgt agaagagcgc gaacgtctgc tgctggcaca gggacggcat gaagtggatt
     2941 tcccgcgcga caacgggatg cgttttcata acggcaacct gccgctgcat gaaaacggta
     3001 tgcaaatcca cgcctataac ggcgatgaag tcgtctacag caaaacttat tattccatcg
     3061 gcggcggttt tatcgtcgat gaagaacact ttggtcagga tactgccaac gaagtgagcg
     3121 tgccgtatcc gttcaaatct gccaccgaac tgctcgcgta ctgtaatgaa accggctatt
     3181 cgctgtctgg tctcgctatg cagaacgaac tggcgctgca cagcaagaaa gagatcgacg
     3241 agtatttcgc gcatgtctgg caaaccatgc aggcatgtat cgatcgcggg atgaacaccg
     3301 aaggtgtact gccaggcccg ctgcgcgtgc cacgtcgtgc gtctgccctg cgccggatgc
     3361 tggtttccag cgataaactg tctaacgatc cgatgaatgt cattgactgg gtaaacatgt
     3421 ttgcgctggc agttaacgaa gaaaacgccg ccggtggtcg tgtggtaact gcgccaacca
     3481 acggtgcctg cggtatcgtt ccggcagtgc tggcttacta tgaccacttt attgaatcgg
     3541 tcagcccgga catctatacc cgttacttta tggcagcggg cgcgattggt gcattgtata
     3601 aaatgaacgc ctctatttcc ggtgcggaag ttggttgcca gggcgaagtg ggtgttgcct
     3661 gttcaatggc tgctgcgggt cttgcagaac tgctgggcgg tagcccggaa caggtttgcg
     3721 tggcggcgga aattggcatg gaacacaacc ttggtttaac ctgcgacccg gttgcagggc
     3781 aggttcaggt gccgtgcatt gagcgtaatg ccattgcctc tgtgaaggcg attaacgccg
     3841 cacggatggc tctgcgccgc accagtgcac cgcgcgtctc gctggataag gtcatcgaaa
     3901 cgatgtacga aaccggtaag gacatgaacg ccaaataccg cgaaacctca cgcggtggtc
     3961 tggcaatcaa agtccagtgt gactaatact tcttactcgc ccatctgcaa cggatgggcg
     4021 aatttatccc cgctttctcg tctgctgtaa tattccccac tacacttcca ctgttgcgtc
     4081 aggcgtttgt cgccatacgc ttacagagtg gcccgcatgc aaaaagcaca acggatcatt
     4141 aaaacctatc gccgtaatcg aatgattgtt tgtacgattt gcgcactcgt tacgctcgct
     4201 tcgaccctga gcgtgcgatt tatttcacag cgtaacttaa atcaacaacg ggtagtacaa
     4261 ttcgccaatc acgctgtaga ggaattagat aaagtactgc ttcccctaca ggcaggtagc
     4321 gaagtcttgc ttccgctgat tggtctgccc tgctctgtcg cccatttgcc attacgtaaa
     4381 caggcggcaa aactccaaac tgtgcgatcc attggcctgg tgcaagacgg cacactttat
     4441 tgctccagca tttttggtta tcgcaatgtg cccgtcgtgg acattctggc tgaacttcct
     4501 gcaccgcaac cacttttacg cctgacgacc gaccgtgtcc tgattaaagg cagtccggtt
     4561 ttgattcaat ggacgcctgc agcgagcagt agcaatgctg gggtcatgga gatgattaac
     4621 atcgacttac tgacggcaat gctgcttgag ccacaactgc cgcaaatcag tagcgccagc
     4681 ctgacggtgg acaaacggca tttgctctat ggtaatgggc tggtagattc ccttccacaa
     4741 cctgaagaca atgaaaacta tcaggtttct tcgcaacgct tttcttttac cattaacgtt
     4801 aatggtccgg gggctccggc gctggcatgg cactatcttc caacacaatt accgctggcg
     4861 gtactgctaa gtttactggt gggctacatc gcctggctgg cgaccgctta ccgaatgagc
     4921 ttttcccgcg aaatcaatct gggcctggcg caacatgagt tcgaattgtt ctgtcagcct
     4981 ttgcttaatg cgcgcagcca gcaatgtatt ggtgtagaga ttttgctgcg ctggaacaat
     5041 ccgcgtcagg gctggatttc accggatgtg tttattccta tcgcggaaga acatcattta
     5101 attgtgccac tgacccgcta tgtgatggca gaaaccattc gtcagcgcca tgttttcccg
     5161 atgagtagtc agtttcatgt tggcattaac gtcgcaccca gccattttcg ccgtggtgtg
     5221 ctgataaaag atctcaatca gtactggttt agcgctcacc cgattcagca actgatcctc
     5281 gaaatcaccg aacgcgatgc cttactggat gttgattatc ggattgcccg cgagctacat
     5341 cgtaaaaacg tcaaactggc gattgatgac ttcggcaccg gcaacagttc attttcctgg
     5401 cttgaaacat tacgtcctga cgtgctgaaa attgataagt catttaccgc agctataggt
     5461 tctgacgcgg ttaactcgac ggtgaccgat atcatcatcg cgctggggca aagactgaat
     5521 attgaactgg tggcggaggg cgtggaaacg caagaacagg cgaagtattt gcgccgtcat
     5581 ggcgtgcata ttttgcaagg gtatttgtac gcacagccga tgccgctacg tgattttccc
     5641 aaatggctgg cgggcagcca accgccgccc gcccggcata atggacatat cacgcccatt
     5701 atgccgttac gttaacgctt actcatcttc atcgtgcgcc ggttgctctt taacaatgcg
     5761 aaccagatca acacgataat cattggcttc aatgatggtg atatgcagtg gccctacatc
     5821 aatcacatcg cccacacggg gaatgtgacc atttgccgag atcacgaggc ccgcgaccgt
     5881 cgcgatatca tcgtcatcgg caaggtgctc aacatcaagc gcctgctgca aggcatgcaa
     5941 atctgtaccg ccttttacca gccagccgtc accatcagta atgatttccg gcgtttcgtc
     6001 agcgtccggg aattcacccg caatggcttc cagcacatcc agcggcgtga ccaagccttg
     6061 taccacacca aactcgttgg tcacgataac aaagctcccg cgagcacgac gcagcacgcc
     6121 cagcaggttg atcggatcga gggtttcagg gacgataatc gccggagacg ccgaagcaat
     6181 cgccgccaca tcaacgccct cttccagcgc caccagcagt tctttagcac gtacgattcc
     6241 gatgatttca tccagttcac cgcgacatac cgggaacagg ctgtgcggtg aggagagcag
     6301 ctgctcgcgg atttcatcga ccccgagatt agcgtcaacc cagcttattt caccgcgcgg
     6361 cgtcatgatc ccgcgcagag aacgcgacgc cagcgtcagt acgccgttaa tcatgtaacg
     6421 ttcttcttcg gcaaatgcac cttccgggat cggcatcggc atcgggttat cggcatcgtg
     6481 ctgaacattg gcctgacgtt tcccgcccat caaacgcagg atggcatcgg cagtacgcgc
     6541 tcgcagcggc aaagtcgact ggtgacgaat aaagttgcga cgcgcaatct ggttaaacac
     6601 ttcgatgatg atcgagaagc caatcgcggc atacaggtaa cctttcggaa tgtggaaacc
     6661 gaaaccttct gccaccagac tcagaccaat cattaacagg aagctcagac agagcaccac
     6721 caccgtgggg tgctggttaa cgaatcgcgt cagcggtttg gatgccagca acataaccgc
     6781 catcgcaatc actaccgccg ccatcatcac cggcagatgg ttaaccatcc ctactgcagt
     6841 aattaccgca tccaacgaga agacggcgtc aaggatgaca atctgtgtga cgaccaccca
     6901 gaaactggcg tagcctttac cgtggccgga atcatgatcg cggttttcca gccgttcatg
     6961 cagttcggtt gttgctttga acagcaagaa tatccccccg aacaacataa tcaggtcgcg
     7021 tccggagaag gagaaatcca tgacggtaaa tagcggtttg gtcagcgtga ccatccatga
     7081 aatcagcgac agcagcccca gacgcataat cagcgccagt gataacccca gcaaacgcgc
     7141 tttatcgcgt tgttttggcg gcagtttgtc agcaagaatg gcgatgaaga ccaggttatc
     7201 gatacccagc acaatttcga gaacaacaag cgtgagtagc cccgcccaaa ttgaggggtc
     7261 cattaagaat tccatgacaa gctcctgctt aaggaatagc tattcgacgc cagaaataat
     7321 gcaggcgtaa cgacaaaatg caaacgaaag gtgcggcata gagtgccaga aaggcaggcg
     7381 ttaaaaggcc tgatgctgaa atgacgtcgg tgacgatcca tactgcgggc tactgcccta
     7441 tactccatga ttgttaaacg ggagttaaac atatcagaga cgcctctgat ttggcaaaga
     7501 tttaccttcc tttgcaaacg aatgtgacaa ggatatttta cctttcgaaa tttctgctaa
     7561 tcgaaagtta aattacggat cttcatcaca taaaataatt ttttcgatat ctaaaataaa
     7621 tcgcgaaacg caggggtttt tggttgtagc ccttatctga atcgattcga ttgtggacga
     7681 cgattcaaaa atacatctgg cacgttgagg tgttaacgat aataaaggag gtagcaagtg
     7741 accattgcta ttgttatagg cacacatggt tgggctgcag agcagttgct taaaacggca
     7801 gaaatgctgt taggcgagca ggaaaacgtc ggctggatcg atttcgttcc aggtgaaaat
     7861 gccgaaacgc tgattgaaaa gtacaacgct cagttggcaa aactcgacac cactaaaggc
     7921 gtgctgtttc tcgttgatac atggggaggc agcccgttca atgctgccag ccgcattgtc
     7981 gtcgacaaag agcattatga agtcattgca ggcgttaaca ttccaatgct cgtggaaacg
     8041 ttaatggctc gtgatgatga cccaagcttt gatgaactgg ttgcgctggc agtagaaaca
     8101 ggccgtgaag gcgtgaaagc actgaaagcc aaaccggttg aaaaagccgc gccagcaccc
     8161 gctgccgcag cacccaaagc ggctccaact ccggcaaaac caatggggcc aaacgactac
     8221 atggttattg gccttgcgcg tatcgacgac cgtctgattc acggtcaggt cgccacccgc
     8281 tggaccaaag aaaccaatgt ctcccgtatt attgttgtta gtgatgaagt ggctgcggat
     8341 actgttcgta agacactgct cacccaggtt gcacctccgg gcgtaacagc acacgtagtt
     8401 gatgttgcca aaatgattcg cgtctacaac aacccgaaat atgctggcga acgtgtaatg
     8461 ctgttattta ccaacccaac agatgtagag cgtctcgttg aaggcggcgt gaaaatcacc
     8521 tctgttaacg tcggtggtat ggcattccgt cagggtaaaa cccaagtgaa taacgcggtt
     8581 tcggttgatg aaaaagatat cgaggcgttc aaaaaactga atgcacgcgg tattgagctg
     8641 gaagtccgta aggtttccac cgatccgaaa ctgaaaatga tggatctgat cagcaaaatc
     8701 gataagtaac gtattgtgtt gattatcagt cagttttcac actttagtca tacgtaaaca
     8761 ggagaagtac aatggagatt accactcttc aaattgtgct ggtatttatc gtagcctgta
     8821 tcgcaggtat gggatcaatc ctcgatgaat ttcagtttca ccgtccgcta atcgcgtgta
     8881 ccctggtggg tatcgttctt ggggatatga aaaccggtat tattatcggt ggtacgctgg
     8941 aaatgatcgc gctgggctgg atgaacatcg gtgctgcagt tgcgcctgac gccgctctgg
     9001 catctatcat ttctaccatt ctggttatcg caggtcatca gagcattggt gcaggtattg
     9061 cactggcaat ccctctggcc gctgcgggcc aggtactgac catcatcgtt cgtactatta
     9121 ccgttgcttt ccagcacgct gcggataagg ctgctgataa cggcaacctg acagcgattt
     9181 cctggatcca cgtttcttct ctgttcctgc aagcaatgcg tgtggctatt ccggccgtca
     9241 tcgttgcgct gtctgtaggt accagcgaag tacagaacat gctgaatgcg attccggaag
     9301 tggtgaccaa tggtctgaat atcgccggtg gcatgatcgt ggtggttggt tatgcgatgg
     9361 ttatcaacat gatgcgtgct ggctacctga tgccgttctt ctacctcggc ttcgtaaccg
     9421 cagcattcac caactttaac ctggttgctc tgggtgtgat tggtactgtt atggcagtgc
     9481 tctacatcca acttagcccg aaatacaacc gcgtagccgg tgcgcctgct caggcagctg
     9541 gtaacaacga tctcgataac gaactggact aacaggtgag cgaaatggtt gatacaactc
     9601 aaactaccac cgagaaaaaa ctcactcaaa gtgatattcg tggcgtcttc ctgcgttcta
     9661 acctcttcca gggttcatgg aacttcgaac gtatgcaggc actgggtttc tgcttctcta
     9721 tggtaccggc aattcgtcgc ctctaccctg agaacaacga agctcgtaaa caagctattc
     9781 gccgtcacct ggagttcttt aacacccagc cgttcgtggc tgcgccgatt ctcggcgtaa
     9841 ccctggcgct ggaagaacag cgtgctaatg gcgcagagat cgacgacggt gctatcaacg
     9901 gtatcaaagt cggtttgatg gggccgctgg ctggtgtagg cgacccgatc ttctggggaa
     9961 ccgtacgtcc ggtatttgca gcactgggtg ccggtatcgc gatgagcggc agcctgttag
    10021 gtccgctgct gttcttcatc ctgtttaacc tggtgcgtct ggcaacccgt tactacggcg
    10081 tagcgtatgg ttactccaaa ggtatcgata tcgttaaaga tatgggtggt ggcttcctgc
    10141 aaaaactgac ggaaggggcg tctatcctcg gcctgtttgt catgggggca ttggttaaca
    10201 agtggacaca tgtcaacatc ccgctggttg tctctcgcat tactgaccag acgggcaaag
    10261 aacacgttac tactgtccag actattctgg accagttaat gccaggcctg gtaccactgc
    10321 tgctgacctt tgcttgtatg tggctactgc gcaaaaaagt taacccgctg tggatcatcg
    10381 ttggcttctt cgtcatcggt atcgctggtt acgcttgcgg cctgctggga ctgtaagact
    10441 gttgtacact accggggcct tttggccccg tttttttatc tggaggatta atgacaatca
    10501 cggacctggt actgattctt ttcatcgccg cactcctggc cttcgcgatc tacgatcagt
    10561 tcatcatgcc ccgccgtaac ggccccaccc tgctggcaat tcctttgctc cggcgtggtc
    10621 gcatcgatag cgttatcttc gtcggattga ttgtcattct tatctataac aacgtcacga
    10681 atcatggtgc gttaataacg acatggttat taagcgcact ggctctgatg ggtttttata
    10741 tattctggat ccgcgttccg aagatcatct ttaaacaaaa aggttttttc ttcgccaatg
    10801 tctggattga atatagccga atcaaagcga tgaacttgtc ggaagatggc gtgttggtga
    10861 tgcaattaga acagcgtcgg ctgttaatcc gcgttcgaaa tatcgacgat ctggaaagaa
    10921 tttataaact tctcgtttca actca
//
LOCUS       DBFIFM010000155        10713 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.155, whole genome shotgun sequence.
ACCESSION   DBFIFM010000155 DBFIFM010000000
VERSION     DBFIFM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.155"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..628
                     /gene="csgD"
                     /locus_tag="N7C74_003526"
     CDS             <1..628
                     /gene="csgD"
                     /locus_tag="N7C74_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HGU2433329.1"
                     /translation="HGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQRSLDDISS
                     GSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWPHINGVFYA
                     MEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHREKEILNKLR
                     IGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            633..1022
                     /gene="csgE"
                     /locus_tag="N7C74_003527"
     CDS             633..1022
                     /gene="csgE"
                     /locus_tag="N7C74_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HGU2433330.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1047..1463
                     /gene="csgF"
                     /locus_tag="N7C74_003528"
     CDS             1047..1463
                     /gene="csgF"
                     /locus_tag="N7C74_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HGU2433331.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1490..2323
                     /gene="csgG"
                     /locus_tag="N7C74_003529"
     CDS             1490..2323
                     /gene="csgG"
                     /locus_tag="N7C74_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HGU2433332.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVTQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2387..2878)
                     /gene="ycdZ"
                     /locus_tag="N7C74_003530"
     CDS             complement(2387..2878)
                     /gene="ycdZ"
                     /locus_tag="N7C74_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HGU2433333.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILSYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTALREQEIK
                     NKA"
     gene            complement(2980..3534)
                     /gene="ycdY"
                     /locus_tag="N7C74_003531"
     CDS             complement(2980..3534)
                     /gene="ycdY"
                     /locus_tag="N7C74_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HGU2433334.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3558..4295)
                     /gene="ycdX"
                     /locus_tag="N7C74_003532"
     CDS             complement(3558..4295)
                     /gene="ycdX"
                     /locus_tag="N7C74_003532"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HGU2433335.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4350..5288)
                     /gene="ghrA"
                     /locus_tag="N7C74_003533"
     CDS             complement(4350..5288)
                     /gene="ghrA"
                     /locus_tag="N7C74_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HGU2433336.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALA
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGWEELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5522..5609
                     /locus_tag="N7C74_003534"
     tRNA            5522..5609
                     /locus_tag="N7C74_003534"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5556..5558,aa:Ser,seq:gga)
     gene            complement(5759..6601)
                     /locus_tag="N7C74_003535"
     CDS             complement(5759..6601)
                     /locus_tag="N7C74_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGU2433337.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6686..6883)
                     /locus_tag="N7C74_003536"
     CDS             complement(6686..6883)
                     /locus_tag="N7C74_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGU2433338.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(6895..7386)
                     /locus_tag="N7C74_003537"
     CDS             complement(6895..7386)
                     /locus_tag="N7C74_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGU2433339.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGNGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(7383..7757)
                     /locus_tag="N7C74_003538"
     CDS             complement(7383..7757)
                     /locus_tag="N7C74_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGU2433340.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(7804..8178)
                     /locus_tag="N7C74_003539"
     CDS             complement(7804..8178)
                     /locus_tag="N7C74_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGU2433341.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(8341..8562)
                     /locus_tag="N7C74_003540"
     CDS             complement(8341..8562)
                     /locus_tag="N7C74_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGU2433342.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(8625..9101)
                     /locus_tag="N7C74_003541"
     CDS             complement(8625..9101)
                     /locus_tag="N7C74_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HGU2433343.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(9117..9602)
                     /locus_tag="N7C74_003542"
     CDS             complement(9117..9602)
                     /locus_tag="N7C74_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU2433344.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(9693..10511)
                     /locus_tag="N7C74_003543"
     CDS             complement(9693..10511)
                     /locus_tag="N7C74_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU2433345.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            10605..10713
                     /locus_tag="N7C74_003544"
                     /pseudo
     CDS             10605..10713
                     /locus_tag="N7C74_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439280.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2696 a         2689 c         2686 g         2642 t
ORIGIN      
        1 tcatggtcat acattattgt tgatcactaa accttctttg caggcgacag ctctcttgca
       61 gcaccttaaa caatcgctgg caattacagg aaaattacat aatattcaac gttctctgga
      121 cgatatctct tcaggctcta ttattcttct ggatatgatg gaagcggata aaaagcttat
      181 ccattattgg caggatactt tgagcaggaa aaacaacaat atcaaaatat tgttgctaaa
      241 tacgcctgaa gattacccgt accgcgacat tgaaaactgg cctcatatca acggcgtttt
      301 ttatgccatg gaggatcaag aacgcgttgt caatggattg caaggcgtcc tgcgcggcga
      361 atgctacttt acgcaaaagc ttgccagtta cctgattaca cattctggta actatcgtta
      421 taacagcacg gaatcagccc tccttactca tcgggaaaaa gagatcctga ataagctgcg
      481 tatcggcgcg tctaataacg agatcgctcg ttcgttgttc atcagcgaaa atacggttaa
      541 aacgcatctt tataatcttt tcaagaagat agccgtaaaa aaccggacac aagcggtttc
      601 ctgggcaaac gataacctca ggcgataaag ccatgaaacg ttatttacgc tggattgtgg
      661 cggcagaatt tctgttcgcc gcagggaatc ttcacgccgt tgaggtagaa gtcccgggat
      721 tgttaactga ccatactgtt tcatctattg gccatgattt ttaccgagcc tttagtgata
      781 aatgggaaag tgactatacg ggtaacttaa cgattaatga aaggcccagt gcacgatggg
      841 gaagctggat cacaataacg gtcaatcagg acgttatttt ccagactttt ttattcccgt
      901 tgaaaagaga cttcgaaaaa actgtcgtct ttgcactgat tcaaactgaa gaagcactaa
      961 atcgtcgcca gataaatcag gcgttattaa gtacgggcga tttggcgcat gatgaattct
     1021 aaataaaaaa ttgttcggag gctgcaatgc gtgtcaaaca tgcagtagtt ctactcatgc
     1081 ttatttcgcc attaagttgg gctggaacca tgactttcca gttccgtaat ccaaactttg
     1141 gtggtaaccc aaataatggc gcttttttat taaatagcgc tcaggcccaa aactcttata
     1201 aagatccgag ctataacgat gactttggta ttgaaacacc ctcagcgtta gataacttta
     1261 ctcaggccat ccagtcacaa attttaggtg ggctactgtc gaatattaat accggtaaac
     1321 cgggccgcat ggtgaccaac gattatattg ttgatattgc taaccgcgat ggtcaattgc
     1381 agttgaacgt gacagatcgt aaaaccggac aaacctcgac catccaggtt tcgggtttac
     1441 aaaataactc aaccgatttt taagccccag cttcataagg aaaataatca tgcagcgctt
     1501 atttcttttg gttgccgtca tgttactgag cggatgctta accgccccgc ctaaagaagc
     1561 cgccagaccg acattaatgc ctcgtgctca gagctacaaa gatttgaccc atctgccagc
     1621 gccgacgggt aagatctttg tttcggtata caacattcag gacgaaaccg ggcaatttaa
     1681 accctacccg gcaagtaact tctccactgc tgttacgcaa agcgccacgg cgatgctggt
     1741 cacggcattg aaagattctc gctggtttat accgctggag cgccagggct tacaaaacct
     1801 gcttaatgag cgcaagatta ttcgtgcggc acaagaaaac ggcacggttg ccattaataa
     1861 ccgaatcccg ctgcaatctt taactgcggc aaatatcatg gttgaaggtt cgattatcgg
     1921 ttatgaaagc aatgtcaaat ctggcggggt tggggcaaga tattttggga tcggtgccga
     1981 cacgcaatac cagctcgatc agattgccgt gaacctgcgc gtcgtcaatg tgagtaccgg
     2041 tgagatcctc tcctcggtga acaccagtaa gacgatactt tcctatgaag ttcaggccgg
     2101 ggttttccgc tttattgact accagcgctt gctggaaggg gaagtgggtt acacctcgaa
     2161 cgaacccgtt atgctgtgcc tgatgtcggc tatcgaaaca ggggtcattt tcctgattaa
     2221 tgatggtatt gaccgtggtc tgtgggattt gcagaacaaa gcagaacggc agaatgacat
     2281 tctggtgaaa taccgccata tgtcggttcc accggaatcc tgacagataa aaatgccgga
     2341 attaataatc cggcatttac tgtttaagcg aaagcgtgag tgcccctcac gctttatttt
     2401 tgatttcctg ctcacggaga gccgtctttg cactacgcgc cgccagccac aggccgctgt
     2461 ttttcattgc gtaaccaaat atcagcccaa gcgccagcga aggtaacacc agtttccagt
     2521 caccctgccc ggcaaacgtc gcgcatgcgc ctataaacgt ccccggaaca aatgaaagca
     2581 gcagctgttt ggcctgaata cacatcagaa acgcgacaat accggttatg acataactga
     2641 gaatttccag atgtggcgcc agtgcactac cgtaaataat gaccatcgcc cacaccacgc
     2701 cactaagcag agttgcagcg gagatcgcca gccctttcag cccaccttgc gggcaggcaa
     2761 agtaggccgt acagcccagg aagcccgccc agcttagtaa gcccagggaa acagccaccc
     2821 atccccagat accggagaga atgcctgttg tgattgcaat agagagaagt atgttcatgc
     2881 cgcgcatgat agcagaaaac gcggccatga atgagatcga gcacacattt tatgcaacag
     2941 caaattatct gttgcagtta tgagtgacgc atatcacatt tactcttcag aatcttcttc
     3001 cagctcgtcc cacattgcac taatggcatc gcgggttagc ggtgccatgg tgcgccagaa
     3061 aggcgtggtt gcatgggcct ccactttgcc aaggaacgca ccacaccagg gtaacagata
     3121 ctcactgaac agtgtttcca gtgcttcgct ctcatcttcc gttgactgat cttccagcca
     3181 ggaagccgca agcagcaatg tgccgatgtg atcggcaggc gtatccgcta atggcatccc
     3241 tcgttcagaa agaaaagcgc gcacttccgc ttccgttgcg tcctcaaccc atgcgctacg
     3301 atatggcggc acggcacatt catcgccgat aaacaacgca ttgtaatcgg cagagacttg
     3361 cgtcatatca cagcttttct gcaaacgtgt cagcaactca tcctgctcca gtggccagtt
     3421 cgcagccagt ttcccctcac gaatcagggt aaacagcggc accagtaaag gatcttgcgg
     3481 ttggcggtaa tagagcgaac ccagcacacg acagaggata gaaaactcgt tcattaatat
     3541 atttccatta ctagtaatta aaggtctgca aattccgcaa tcggtgccat accgcgagat
     3601 tcaaggaagt tcagtaagcg gcgcggagaa acattcaaaa tgcgctctgg cggaaaatct
     3661 accgcgtcga ggattttaag acactcttca aattccccca tggtaaacgc ggtgtgagaa
     3721 tccgagccta atgccaccca acctccagca tcacgtaccg ctgcggctac cgcacgacag
     3781 ttgtcttcac tgcccttacg tgagtgtaaa aatgaggaat tattgatttc cagcgccacc
     3841 tgatgtttcg cggctgcttc agcaacggct ttaacatcta tctcatattt cggatttccg
     3901 ggatggctta ttatatgcac attgccgctg gcgatagtgg cgatcatcgc ttgtgtattg
     3961 gtcgctttgt catgtggtgc aaaaaccggc tcatgaaaac cggcaataat cagatccagc
     4021 gagtcaaaca ttttaccgct gcaatcaatt tcaccatcaa catttttaat gttagcttcg
     4081 atgccgcgca ggatccctac cccatcaacc actcgcggcc agatacgcat gttaatgaag
     4141 tgccagtgat gcggcgcatc ttccatgtcc gggccatgat cggtaatcgc aaaaagttta
     4201 atgccctttt gtttggcctg ggcaatgtaa tcactcaatg tgctatatgc atgtgtgctg
     4261 gcaacggtat gcatatgaag gtcgacggga tacataactc tctcctgtat tcaattcccg
     4321 ccaaggatag caggaatcct gatgctttat tagtagccgc gtgcgcggtc gacttgcccg
     4381 cagaccctct cccctttttc gagctgggca atagtgcgag aaatgtactc cacagcttca
     4441 gcgggacggg taatcgcggc gacatgtggt gttatcgtca cgcgtggatg ttgccagagc
     4501 ggactttcag gcggtaaggg ttcacgatta aaaacatcca acattgcgcc tttaactttg
     4561 ccgctatcca gcgccgcgag caggtcatct tccacgacat gaacaccacg cgccaggttg
     4621 aggagatacg cgccatccgg taatttttcg agtaattgtt gattaataat gccgacggtt
     4681 tcaggggtat tcggtaacaa attaatcaat acccgacatt ggctcagaaa tgcagacagt
     4741 tcttcccatc cggcaaagct ttgcacgcca ggccacgatt tacgggttcg actccagcaa
     4801 cgcagcggaa agcgccaggt ttgcagactc tgagcaactt tactgcccag tacgcctgcg
     4861 cccaaaatgc cgatggtaaa atcttcccga tgatattcag gcagcggttg ccaatgcgaa
     4921 ctattttgct ggatgcgata atcgtcaaaa cgtcgaaacc aatgcagcac ctgactgaca
     4981 gcatattcct gcatttgctc gcccataccg gtatcttcca ggcgaaaaag tggaacagaa
     5041 ggcttcagca tttcagggtg tgcctgtagc ttgctcaaaa tagaatcaac accggccccg
     5101 agtgcgaaca ccgctttaag atcgcgccct gccagcattt caacaggagg atgccaggct
     5161 aaagcataat cagcagagtc attatctccg cttttccatg ctctgactct tgcctgagga
     5221 atagctttgc gcagtgcctc tatccaccat tgggtatcga acgttgggtg ataaaagatg
     5281 atatccatac tgactcccga aaagcgttgt gcgaaattta ttcgcactta tcgttatgat
     5341 ctacaaagac caccagcata acaaatccgt ggtcggtggc caaaaaagca gattttgctt
     5401 attaaaactg catattgatt gaagtttgaa taaacgcgcg attttttaaa aaagtttgtt
     5461 gacctcaggt catgatttcc ctaaattagc gcccgttcca gcaagacagg aacgacaatt
     5521 tggtgaggtg tccgagtggc tgaaggagca cgcctggaaa gtgtgtatac ggcaacgtat
     5581 cgggggttcg aatccccccc tcaccgccag attataagcg catgttattg tttaacatgc
     5641 gcttttttat tttcccttcc agacagtatg ccatcgggta tgccatctga aaccggttta
     5701 tatgccccgc gatcatatct tatcggacaa tttccgatct ttgagcatac tggcaaagct
     5761 atactgccag catatcagga tagtgttttg cgataatatc attcagtctg tcaaccagtt
     5821 ctggttttct gaacgtgata tgcgcggagc ccttcttaaa gtatctgatg ctgaacatct
     5881 catcttcata gctctcttta ccctgaacag actgaatgtg atcatccaga cgaagggtga
     5941 tgtttccccg gttgtcgggg attggtttac cgctgaacag gtgcaacatt ctttccagat
     6001 cggcgagtcg gtcagcctgt tgtccggtga taagatgaaa tccccaccgg tcccatctca
     6061 ccagattgtt gacgataatt ttactgccaa atttacaggg gcaattggtt ttgtaattcc
     6121 agttcagccc tctgaagacg ttgatcaccc ctcgctcaaa cacctcatcc ttattctggt
     6181 gcagttgttc aaatgtgctc agaatgttcg cttcgctgat ttccggaaaa ttatcgtact
     6241 ccagtgaccg gtaccatgta tcacgggcct gcgtatccat taatgacagc atcccggaac
     6301 gttgcatcag gtcacgccag atttcgcgat caagattccg ggtaatggct ttcattgctg
     6361 tttcgggttt ctccatcagc cagcagccgc agcgaaaatc ctgtttcatg gcccagtccg
     6421 gggcagtttt acctccgatg ctttccgtca gcaccgagat ttcagccagt tgtcggataa
     6481 gtgtgtcgat ttgcaacagt gtactctttc gtacagccag aatgtggcga atgctggttg
     6541 agcagataac gtccgtatgc ccgttgacca cttccggacg ggagattgtg atatctgaca
     6601 taatcgatat tcctttttgc agataaatga aaacgctagc cctgtgcagg ctggcgtcag
     6661 aggaatgaag tctggtgagt gagcttcacc ggttctggtg cagaagctgc agatgacgca
     6721 gggtacggac atcctcccgg gcctgttcga tacagggcaa cagtgctgct gaatccgttt
     6781 tatcctcatc gttgtcaaag ataattcccg atccgcagtc gatattgtcc tgcagccacg
     6841 caatcagaat atccagcgct gtttccgtgg ttaatgattt catgttgttc ctggttacca
     6901 gtcgaaagtg ggtaaacccg gcaggcattc agcgctggca tccagatgaa tgagactgac
     6961 gccataacgc cggatgagtg tgacgaccag acgacggaac gtttttgaca gacccaggcg
     7021 tttaagccgc agaaccgggt acgaccacgc atcggtacgc aacagatagc cgttaccggt
     7081 aaaatgaatc cattctgatt caccgaagtc actggtctgg tgtgacagcg agtacagcca
     7141 ggcgttgtcc gtttccgtga tatgtgcggt actgcagcgt atgccggtaa gggtagcaaa
     7201 tggtggcgtg cattctgctg aggtctggtc agattcatca acaacacgaa gggagtagcc
     7261 gttatcgcag accatgttaa tcagttcagc gagctcaata ccatcgacgt caacgacaat
     7321 gcgccccatg ttcagtgcct gtacgttaac gctgtcggct tccagtgtca gggccagttt
     7381 catcatttcg cctccggata cttacccagg gtaatgttat ttaccgttct gtaattgtcg
     7441 cgggtcatca ggccggtcgc ccggcgagcc cggaggatat caatactgtt tattaactga
     7501 gagcggggac aggtgctgaa tcccggctgg tcggtacgca ccagcgcgta tttttccacg
     7561 agaaaattaa ctgcatcaca cagtgaaatg cctgcctcaa tatgctgctc aatcacacgt
     7621 tcatcggcaa acggtgtgtc attcagtgtg aggccataat gctggtccag cagtcggatg
     7681 agcagtgtct gccagatggt gacgggagac gggcagcgag atacctcccg tacatgtgtg
     7741 tcggataatg ttttcatctt aagttctctg taactggtag tgctaaaggt gagagcttcg
     7801 gggttatgag gtggttgcgg gtgctggtgt cggataaaca gccagataaa cgtaaccaca
     7861 ggagccgagg gtgtcggctt cgcaggtcag cccttttgca ttcagcgtga cggtatgctg
     7921 atatcgggga ttcagttcac cgctggtgag catgagttcc agttgtttca tcagcagcgg
     7981 aaaggcctgg tccagatggt atgcatctgc gtcgctgaag cggcctctga taccggcgcg
     8041 gtcggcaagg taatgcaggc ggttaccctc ttgcaccaga cgagcgccaa aacagggcct
     8101 cactgtgcag ggaagccccc accaggggcg gtcgtgattg tcatcgggat gagttatccc
     8161 ggagagtttg tctgacacga taaaatcctc tctgaaaagt ggttaagaat ggtttaacga
     8221 tgacaggtca gccggcgcgc tgaccggttt cggtgaagat gtatgcattg atgagaatgg
     8281 cctcatccac agcggcgtct gaggtcatct ggtcgtactc gtttcccggg tgacggaaca
     8341 tcagttcagg gtgacactca tcagccggac atacgggcca aaactgtcct tacggcgttc
     8401 tgcaaacacg gcaagaacgc cgggaatatc ctgcacttca cgaccagtat acgcttcagc
     8461 actgccgtgc cagcgatact taccggtaca gaacggaaaa agacgggatg caggatgctg
     8521 ttggtgaata cgcatggctt caccacgggt gatgattctc atgatgtgat acctctgaag
     8581 acagaagata aaagtgaaaa caggtgtgat gtggttgtga cgggttaaag cagaccatgt
     8641 tcggcaaagg agaaaacctg gttgccaccg actatcagat ggtccggcac ccggatatcc
     8701 accaggccca gtgcctgtac cagacgttcg gtgataagcc ggtctgcctt actgggtgtg
     8761 acttcaccgg acgggtgatt gtgcgccagc accacggcag cggcattgtg gtacagggcg
     8821 cgtttaatca cttcccgggg atgaacttcc gtacggttga tggtgccggt gaagagggtt
     8881 tcaccggcaa tcagctgatt ctggttgttc agatacagca cccggaactc ttcccgctcc
     8941 agtcccgcca tgttcagaat cagccattcc cgtgccgcac gggtggaggt gaaggccacg
     9001 ccgggttcat gaagatggcg gtccagggtt tgcagggccc gcagaatgag actgcgctcg
     9061 ccgggcgtca tctctccggg cagaaaggaa agctgctgca ttgtccctgc ctccattcag
     9121 tcaatgatgc gcataatggc gctgcattcc ggatgctgca gggcgtaatc ccgcagccgg
     9181 taatagtgga ccgtcatggc ataacactcc gtacgacagg catgatgact gtacgtcatc
     9241 agacaggcag caataccggc ggcttccggg ctcatttcag cgcggttgcc gttcatggca
     9301 ttgaacagta cccatgtttc gtcatcatcg tcatccggtt cgggtgccat aaatgccccg
     9361 ccgttgttca gggtgtacag attccagata ccaccgcagt agtcttcgca aagacggtcc
     9421 atccagccga agacacgggg ctccagggtc acccactgtg gaatgaggcc aaaatgctgc
     9481 ggccagaagc tgatgcgctg ttcatcaggg acgggagtgg caaccagctg aggtgggtta
     9541 ttccctgatg cagtggttac ggaaacagaa ggtgtggtgg aattatgcga aacggttgtc
     9601 atgaggttat tccttataaa aagtaaatga atggaagaag ccccggggca ggaacagatg
     9661 tgagtcagga ctgcactttc agtgaaacgg catcagcgca tgctctccag cagcgtttct
     9721 gccatcaccc acaatgcgcg attgagctta atgtcggtat caatgctgtg aatggcccgg
     9781 gtatggatac gttttccttt tgcactgcga ccggaaatcc cgcctttcag catattctcc
     9841 tggatggtct gataagcact ccacaggtcc ttaccgtaat cctcccggcg tcgtggtgtc
     9901 agaatgtcgg cggtggtgac gggctgatgt tcgtcaccat aacggtaagt cagtgccgcc
     9961 tgtgccagcg cctggcgtgc cggtggcggc aggaccagcg actgcatggc atcacgcttc
    10021 tcctctatcc ggtcaaaaac gcccaccacc tcgtaagccc cttcaatgac tctgtccacc
    10081 acatttcccc ggtgtggaac acgcaattct cccagagact gaccgcagac cagtccattg
    10141 gtacaaatgg cacgaaaata tccgggtaac atctggtagc tggatgaacc gtcatgggag
    10201 ttgagcagaa taatttcagg gacatgctga ccggttatct gtccggcccg ccgcagacgc
    10261 agcatgtgtt ttgtgtattc ccggcggctc tggtcgcgca cgcgggtctg gcaggcaaag
    10321 aacggctgaa agccttcctg ctgcagattt tccaggacag tgatagtggg aatgtacgca
    10381 tagtgttcac tgcgagaggt atgtcggttt tccccaaaaa tactcggtac atggcgcatc
    10441 agttcttcgt gtgtcagcgg acgatcacga cgtatctggt tcgcataacc aaaacgactg
    10501 gctaatctca tgatttgctc cttatgaatg attaagcgat ataaatgaaa aagccgcgtc
    10561 tcccggagaa gacgcggcct gatgggtgaa atgaatgagg tttattgtct gagaagccct
    10621 tcactggcga accacgtatt aagctgtatt ccggcatcgc cagtgcaact gaccttcagc
    10681 attgcggata acccgccgga aatatgttca ctg
//
LOCUS       DBFIFM010000156        10650 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.156, whole genome shotgun sequence.
ACCESSION   DBFIFM010000156 DBFIFM010000000
VERSION     DBFIFM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.156"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..193
                     /locus_tag="N7C74_003545"
     CDS             <1..193
                     /locus_tag="N7C74_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433346.1"
                     /translation="KAIRQDGLFALVKKMPDATLRVLYSHICQIQQRIRFPQLAYFHT
                     CPPYQKFILKLLNNHFPAN"
     gene            310..1608
                     /gene="kgtP"
                     /locus_tag="N7C74_003546"
     CDS             310..1608
                     /gene="kgtP"
                     /locus_tag="N7C74_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HGU2433347.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDCYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDSALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1605..1928)
                     /gene="yfiM"
                     /locus_tag="N7C74_003547"
     CDS             complement(1605..1928)
                     /gene="yfiM"
                     /locus_tag="N7C74_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HGU2433348.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKEFWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1974..3329)
                     /gene="pssA"
                     /locus_tag="N7C74_003548"
     CDS             complement(1974..3329)
                     /gene="pssA"
                     /locus_tag="N7C74_003548"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HGU2433349.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLLDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3443..6103)
                     /gene="pat"
                     /locus_tag="N7C74_003549"
     CDS             complement(3443..6103)
                     /gene="pat"
                     /locus_tag="N7C74_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HGU2433350.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATEIAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAKAHLLLQQAIAEGATSLDTHEVQPILQSYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMTWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6135..6833)
                     /gene="tapT"
                     /locus_tag="N7C74_003550"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6135..6833)
                     /gene="tapT"
                     /locus_tag="N7C74_003550"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HGU2433351.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6902..7321)
                     /gene="trxC"
                     /locus_tag="N7C74_003551"
     CDS             complement(6902..7321)
                     /gene="trxC"
                     /locus_tag="N7C74_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HGU2433352.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7528..8565
                     /gene="yfiF"
                     /locus_tag="N7C74_003552"
     CDS             7528..8565
                     /gene="yfiF"
                     /locus_tag="N7C74_003552"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HGU2433353.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8613..9302)
                     /gene="ung"
                     /locus_tag="N7C74_003553"
     CDS             complement(8613..9302)
                     /gene="ung"
                     /locus_tag="N7C74_003553"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HGU2433354.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQHGETPI
                     DWMPVLPAESE"
     gene            9607..9990
                     /gene="grcA"
                     /locus_tag="N7C74_003554"
     CDS             9607..9990
                     /gene="grcA"
                     /locus_tag="N7C74_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HGU2433355.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10046..10633)
                     /gene="eamB"
                     /locus_tag="N7C74_003555"
     CDS             complement(10046..10633)
                     /gene="eamB"
                     /locus_tag="N7C74_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HGU2433356.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFHQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWIVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLIYCAVRIFY"
BASE COUNT         2625 a         2709 c         2750 g         2566 t
ORIGIN      
        1 aaaggccatc cgtcaggatg gcctttttgc gttggtgaaa aaaatgcctg atgcgacgct
       61 gcgcgtctta tactcccaca tatgccagat tcagcaacgg atacggtttc cccaacttgc
      121 ctacttccat acgtgtcctc cttaccagaa atttatcctt aagctcctca ataaccattt
      181 tcctgctaac taaattcatg gttaaggttg cataatgata cgtaacaaat gtataacatt
      241 ttctttgcca aaagaaataa acaaaagcga ccgacaaaag catcggatta cggcaggaga
      301 cataatggca tggctgaaag tactgtaacg gcagacagca aactgacaag tagtgatact
      361 cgtcgccgca tttgggcgat tgtgggggcc tcttcaggta atctggtcga gtggttcgat
      421 tgctatgtct actcgttctg ttcactctac tttgcccaca tcttcttccc ttccgggaac
      481 acgacgactc aactactaca aacagcaggt gtttttgctg cgggattcct gatgcgccca
      541 ataggcggtt ggctatttgg ccgcatagcc gataaacatg gtcgcaaaaa atcgatgctg
      601 ctatcggtgt gtatgatgtg tttcggctca ctggtaatcg cctgtttgcc tggttatgaa
      661 acaatcggta cgtgggctcc ggcattattg cttctcgcgc gtttatttca gggattatcc
      721 gttggcggag aatatggcac cagcgccacc tatatgagtg aagttgccgt tgaagggcgc
      781 aaaggttttt acgcatcatt tcagtatgtg acgttgatcg gcggacaact gctagcccta
      841 ctggttgtcg tggttttaca acacaccatg gaagactctg cactcagaga gtggggatgg
      901 cgtattcctt tcgcgttagg agctgtgtta gctgttgtgg cgttgtggtt acgtcgtcag
      961 ttagatgaaa cttcgcaaca agaaacgcgc gctttaaaag aagctggatc tctgaaagga
     1021 ttatggcgca atcgccgtgc attcatcatg gttctcggtt ttaccgctgc gggctccctt
     1081 tgtttctata ccttcaccac ttatatgcag aagtatctgg taaatactgc gggaatgcat
     1141 gccaacgtgg cgagtggcat tatgactgcc gcattgtttg tattcatgct tattcaacca
     1201 ctcattggcg cgctgtcgga taagattggt cgccgtacct caatgttatg tttcggttcg
     1261 ctggcagcca tttttaccgt tcctattctc tcagcattgc aaaacgtttc ctcgccttat
     1321 gccgcttttg gtctggtgat gtgtgccctg ctgatagtga gtttttatac atcaatcagt
     1381 ggaatactga aggctgagat gttcccggca caggttcgcg cattaggcgt tggtctgtca
     1441 tatgcggtcg ctaatgctat atttggtggt tcggcggagt acgtagcgtt gtcgctgaaa
     1501 tcaataggaa tggaaacagc cttcttctgg tatgtgacct tgatggccgt ggtggcgttt
     1561 ctggtttctt tgatgctaca tcgcaaaggg aaggggatgc gtctttagtg acgggtcagt
     1621 tgccagacgg tatagccggt gcttgcaccg gcgacatccc aggccaaatc cttccagctc
     1681 cagccgctcc cttcggggcg gctatcccaa aactcttttg acgcccccaa actgacagag
     1741 aacatcaaac caaacatggc actgcgatcc cggctcatcc cctgatgctg tgaatattca
     1801 tttccggcgg cggaaagcat cgccgaggcg ataaagtgtt gagctttatc ctgcccgctc
     1861 cagctatcgt tagccatatg gctgcatcca gaaagtaaca atagcgaaca gacaaaaaga
     1921 atacgcaaaa cgtggcctcc aaaaaacaaa ccccgtacag gacggggttg tgattacagg
     1981 atgcggctaa ttaatcggtc gatgcggata cggcgcaaac ggcggatgag tttacgaacc
     2041 ttcaccggat aatcggcaat actttgcaga tcgcgatagt gcttaacgat ggtggtatgc
     2101 tcgcggatca gctccagttc tttctctcgc tgcggcgcaa gctccagttg tggatcgtgg
     2161 atcaaaatgg cgttttccag atccagacgc caggcgcgcg ggttcaggtt attaccggtg
     2221 atcaacatcc acttatcatc aacccacatc ccttttaggt gataggtgtt gtcgtcatct
     2281 ttccataacc gaaccactag ctggtcagta ttgacgtaat actgcaaacg gctcaggaaa
     2341 cgacgcagat tgatctcata gagataaggc aatgcgccaa ttatcttgaa aggttcatct
     2401 tccggaatgt agaagtcgtt cgcggtttta tcaccaacaa taatttcgac ctttttccct
     2461 tcgcgcagca actggataat attgcgcaca aggattgctg gcaggttgaa gtatggcgta
     2521 cagatggtta gtttctgctc ggcacaaggc ataagatgga aaatggtctt gttcaacaga
     2581 ctcgatttcc ccagccccac tagcggcgtt acggaaagct gatcgttgtc ggcatcgccc
     2641 tggaaatgat aagcggcatc acgcagctcc tggcggaaca gacgaatatc gttcttgatt
     2701 tccgggcttt ttggccgatt aacatcatcc agacgattaa cgccgcggcc attcataata
     2761 ttctgtgtaa cccattcaaa cataatgtct gacatcttac ggttacggat aagatgataa
     2821 cggtcgtagc gatatttatc gtgctgatgc aggtaaacat cgttcaggct ggcaccgcta
     2881 taaagtacgc tatcgtcgat gataaagcct ttaaagtgca gaacaccaag ggcttcacgg
     2941 gtattgattg gaacgccata aaccggaaca tctacgcccg gattttcctg cgccatgcga
     3001 cagtaccagt cagcgttagt gttagatgcc gcagcgccaa tgcgtccacg ttgtgcacga
     3061 tgccagtcga gcagcacccg cacatccagt tccggacgct gccttttagc ttcatacaac
     3121 gcgttcagaa tgcctttgcc accgtcatcc tgttcgagat acagggcgac aatgcaaatg
     3181 cgctgcttcg cgctggctat tttttccagc agcgtctccc ggaagtcggc gggagcgtaa
     3241 aagaaatcga catcatcaac tgattgagaa atcttgggta gttgggcaag gtgttgttga
     3301 tgtttattac gcttaaattt tgacaacatc acagtgcatt tcttctctgt tcattgaagg
     3361 gtcctctgtg caatgcagac gacataagcg tgcaataata acaccagtcc cgattaagtg
     3421 gtcaacattt ccagtacctt actcatgatt cctcgcgttg ggcaagattt agcgtaagcc
     3481 caacgatccc ctcttcgagc tggatatcaa cgttaaaccc gagcttgcgg gccagcgcca
     3541 ccatgccacg attgtttggc atcgtaatac cattcagacg ttgtagtccg tgatctcgcg
     3601 tataggtaat caacttttcc attaagcgtc gacccaagcc taaccctttg agatccgagc
     3661 gaaccagcac ggcaaattcg gcatcgatgt tatcagggtc ggagatcgca cgcgtgacgc
     3721 cgaggatctc ttccgtttga tcaattcgtc gtaccgctac aaacgccatt tcccgatcgt
     3781 agtcgatctg cgtcatgttg gctaaatctt catgggtaaa ttcgttgatc tcgctaaagt
     3841 agcggtaata aagatcttct ttggtgaccc gcgaaatgaa ttgctgaagt tgtggctcat
     3901 cttctggcaa aatcgggcgg aacaagcagc gttcaccgtt tttcaattct acccattctt
     3961 ccagctgatg cggataaggg cgcactgcca gccgactctc gttatcgcct tcaaacggcg
     4021 cgatatccag agtgacatcc agcgcggtaa attcactgcc agaagccagc aaaggatgaa
     4081 tatccagacg ctgaatttcc gggcaatcga caatcaagtt ggaaacctgc accagaagct
     4141 ggctcaagcc tgcaacatcc aatgggcgta gcgcactgcg cgcacgaatc tttttacttt
     4201 tgatcccctg aataaccaga tagcgggcca ggttcatatt cagcggcggc agtgcgacga
     4261 cggcttgatc ttcaggacgc cactccacac cgccttcacc cagcatgatc aacggcccaa
     4321 aaaccggatc gtgctcaacc acaacccgca actcctgagc gccagcacgg ttagccatac
     4381 tttgcaccaa caggccgtgg acccgcgcct gcggccaggt catttttacg cgatcgaaaa
     4441 tagcgtttgc cgcttgctgg acttcattgg ctgtacgcag gtacagcatg acgccctgaa
     4501 cttccgattt atgtggaata tccggcgaac gcaatttcag cgccaccgga taaccaatct
     4561 gttcggcaat atgcaccgct tcggtgctat cgctggcaat ccaggtaggg agcgtgttca
     4621 tcccatacga ttgcaggatg ggctgaactt catgggtatc gagcgacgta gccccttcgg
     4681 caatcgcctg ttgcaacaga agatgcgctt ttgcggtatt ggaagtcaga ttgctgggca
     4741 acgccggcgt ttcgcgtagt tgcttctgat tacgccggta ctccaccata tgcataaaag
     4801 cagtgatggt tccttccggg gtacggtagg tcggcagccc ggcttcgctg aataaacgtc
     4861 gtgcctcttg cgaggagtgc tcgccgcacc agttcgtcag cagagaaaca tacttgctgc
     4921 ggggatgatg ctttaccgct tcaattaata cttgcgcgat ttctgttgcg ggagcagcgg
     4981 cgctgggcga atgaataacc atcagcgcat caaaatcctg gctgtggagc agaatgtcca
     5041 gcgttttgac ataatgctcg ctgctggcgt catcgcgcag atcgagcggg ttagagattg
     5101 ccacatgtcc tggcagtgca tcgcgcagtt tctggcaggt ttcttcgctt agcgttgcca
     5161 gcttgccatt gcgtgaccat aaggcatcca gcgccagcgc ggcaggcgca gcaccgttgc
     5221 tgataatcat caaccggtcg ccacgcagcg ggcgcatatg gctgagggtt tccaccgccg
     5281 aaaacagctc gtgggtatcc tgcacccgca acaaaccggc acgctgaatc gccgcatccc
     5341 acgccggatc cattcctgcc gtcgtgttga gcagtcgctg tgctgccggg ctacgtccgc
     5401 ttttaatcac cagaatcggt ttattacgcg aggcactacg ggccgccgaa acaaagcgtc
     5461 gcgcgtcgct taactgttcg agatagagca ggatggcgct ggttttactg tcgcgtgcca
     5521 gatagtcaag caattcatca acgtcgatat ctaggctgtc gccgagcgca ataaagtagg
     5581 aaaagcccat ctcacgctgt tgcgcccagt cgaggatggt gttggagacg gcagccgact
     5641 gtgaaataaa cgccagcttg ccgcgtttaa tcggcacagg cgaaaagctg gcattcagac
     5701 cttgccaggg agccagtaaa cccagactgt ttggtccaag caggcgcatg ttatggcgca
     5761 gggcgcaggc gcggagatct tcgtgttgcg atgccggggc ggaaagaata atgcaggttt
     5821 tacagccttt ctcgccgagc tcttccagaa gagcaagatt acggctggca ttggtacata
     5881 aaaccgcaag gtcgggtgta aagggcaagc tggcaatatc cggccaggcc aacacaccca
     5941 acaccgcttt ccaggctggc gtcaccggga gtaccggtcc gttaaagcct cccgccagca
     6001 ggttacgcat catcaggtaa cctgcgcgat tgggtttcat cgacgcgcca attaccgcta
     6061 tcgattttgg tcgcagtagt gcttccagtc ctcgctgact cataccggtc tcccgcagaa
     6121 gtgaccgaat gattttaaac gctttctaac tgttctgctg tgatgctacc cagatgttgc
     6181 gtttttcctg ccagatagcg cgttttaaag cgggtgaagt gctcgcctaa ccctgctgcc
     6241 gccccggtat cgccggccat atctaacagt gcgatggcta cctcggcagt acaatattgg
     6301 ccttcagcct gggcttcacg caggcgatag gcagaaagtc gggaaagatc gacggaaatg
     6361 acgggaagat tatccagata cggactttta cgaaacatct tgcgagcttc cggccaggta
     6421 ccatcgagca tgataaacag cggtggctta ccggcaggtg gtgtgaagat cacttcccgt
     6481 tgctcatcag catacgaggc gggaaagacc accattggct gataatccgg gttttgtacc
     6541 agatccagca aatcctgcga gggttcggta cgcgaccatt gaaacgcaac ggtatcaggc
     6601 aaaatatcag caatcagacg cccggtatta ctgggcttca ttggctcggt gtcgaacatc
     6661 agcaaacaga agcgactttt tgcttgtgct ggggtaattg tcgaacagag acataatttc
     6721 tctggcaaaa gacagcgttg gcagcgacga acgcgattac cgcgggcaag aaaaggacgt
     6781 gttgcgcgcg caatacgctc ggcgcgtaac tggagaacag cgttttcggt cataagagag
     6841 cgtcgaaaaa acgccattgt cgcagaggag aaaacggggc acaagatgcg ccccggtaag
     6901 attaaagaga ttcgttcagc cagctatcga acggcgcttt cggtactgcg ccattaagca
     6961 tgtcgacaac ctgaccgttt ttgaaaatca tgatcgtcgg tatactacga attccaaagc
     7021 gactgctcaa ttcacgttca gcttcggtat tcactttcac aaagcgcact ttaccgctac
     7081 gctcttgcgc gacatcttca aaaattggtg cgaaattacg gcaggggccg caccacggtg
     7141 cccagaagtc gatcaccact ggtagatcat ctttcagcaa tttgtcgagc gtttcaccgg
     7201 tcgcattaat cacctctccg tcaaacaagt cgtgaccgca gcgtccgcat tttgccgcat
     7261 cttcgatccg atcgtcggga atgcgattga tggcctgaca atgggtacaa acggtattca
     7321 taactaacct cgggatgagt aaggagactt atcggcaaca acgccggtat gtttctaata
     7381 tgttacatat tatcggggac attgttccag acaacaatct gttttattca ataggtatga
     7441 tagtcgcagg ctttggtacg aaatcatggc gaaccgcgcg gacttcgggt aatctgcgcg
     7501 cttcgcgcag cgctggtgga gaaaagcatg aacgatgaaa tgaaaggtaa aagcggcaag
     7561 gtcaaagtga tgtatgtccg cagtgatgat gattctgata aacgtaccca caacccgcgt
     7621 accgggaaag ggggcgggcg tccaggaaaa tctcgtgctg acggtggccg tcgccccgcc
     7681 cgcgatgaca aacagagtca gccccgtgac cgcaagtggg aagattcgcc gtggcgcacg
     7741 gtttcccgcg cgccgggtga tgagacgccg gaaaaggccg atcacggtgg catcagtggt
     7801 aaaagtttta ttgatccgga agtgttgcgt cgtcagcgtg cggaagaaac ccgcgtctac
     7861 ggcgaaaacg cctgtcaggc actgttccag agccgtccgg aagcgattgt tcgcgcctgg
     7921 tttatccaga gtgtaacgcc gcgttttaaa gaagccttgc gctggatggc agcaaaccgc
     7981 aaagcgtacc atgtggtaga tgaagcggaa ttgacaaaag cgtcaggcac ggaacatcac
     8041 ggtggcgttt gcttcctgat caaaaagcgt aatggcacaa ccgtgcagca gtgggtaagc
     8101 caggcaggcg cgcaggattg tgttctggca ctggaaaacg aatctaaccc gcataacctg
     8161 ggtggcatga tgcgcagctg cgcgcacttt ggcgtgaaag gtgttgtggt gcaggatgcg
     8221 gcactgctgg agtcgggggc ggctatccgt accgcagaag gcggcgcaga gcacgttcag
     8281 ccgattactg gcgacaacat tgttaacgtg ctggatgatt tccgtcaggc gggttacacc
     8341 gtagtgacaa cttccagcga gcagggtaaa ccgctgttca aaaccagtct gccagcgaaa
     8401 atggtactgg tgctgggtca ggaatatgaa gggttaccgg atgccgcacg cgatccgaac
     8461 gatctgcgcg tgaagattga tggtactggc aacgttgccg ggctgaatat ctctgtcgca
     8521 accggcgttc ttcttggcga atggtggcgt cagaataaag cctgataaat cagccgggtg
     8581 gcaactctgc catccggcat tttcccgcaa atttactcac tctctgccgg taatactggc
     8641 atccagtcaa tcggcgtctc gccatgttgt tccagccact gatttgccag cacaaaatgg
     8701 ttgcagccaa agaatccacg atgagccgaa agcggcgacg gatgcggtgc tttcagtaca
     8761 tgatggcgtt gcttatctat aatcgcccct ttcttttgcg catgcgatcc ccacaacaaa
     8821 aacaccacgc cttcgcgatg ctggttaatc aggctgatca ctttatcggt gaaggtttcc
     8881 cagccgaggc tggcgtggga atgcgcctga cctgcgcgta ccgtcaacac agtattgagt
     8941 agcagaacgc cctgacgcgc ccagctttca agataaccat gattagggcg ggtgaagccc
     9001 ggaatagtat tttccagctc tttatacata ttcagcagtg acggaggagt ggcgattccg
     9061 gggcgaacgg aaaatgccag cccatgcgcc tgtcccggtc cgtgataagg atcctggccg
     9121 agaatcacca ctttaacgtc acccaactcg gtaaagcgga acgcgttaaa gacatctttt
     9181 tgtggtggat agatagtgac gccggactgc cgctcgctgg cgacggtctg aagggtatta
     9241 agaaaatagg gttgctgctt ctcttcagcc agcacgtcat gccaggttaa ttcgttagcc
     9301 atctcgctct cctgcgaatc ttcaatccgc ctagcttaac tgcttctttc tacagaacaa
     9361 aatcatgctc attttttaga cttaaacgag tgcaaaaaaa tgtaaattgg taaaatttca
     9421 cacccgcata agttgatgta aaacaataaa atctatttgc gaccacactt gatgtgtggt
     9481 ttttattgat ttaaatcaaa gattcaaggg tgtttgagga gtatatatac actcaagcaa
     9541 caatggtttt accaattggc cgcgacaggc tgaacaaatc aaataatttt gccggggagg
     9601 catcacatga ttacaggtat ccagattact aaagccgcta acgacgatct gctgaactct
     9661 ttctggctgc tggacagcga aaaaggcgaa gcgcgttgca tcgttgcgaa agcaggttat
     9721 gcagaagacg aagtggttgc agtaagcaaa ctgggtgaca ttgagtaccg tgaagttcca
     9781 gtagaagtga aaccagaagt tcgcgttgaa ggtggtcagc acctgaacgt taacgttctg
     9841 cgtcgtgaaa ctctggaaga tgcagttaag catccggaaa aatatccgca gctgaccatc
     9901 cgtgtatccg gttatgcagt tcgctttaac tctctgactc cggaacagca gcgcgacgtt
     9961 atcgctcgta cctttactga aagcctgtaa ggcttaagta gcatgttact aagcggaaga
    10021 gggcgacctc ttccgctttt tttcattaat agaaaatgcg taccgcgcaa tagatcagca
    10081 gcagcgccag cacgatattt aactggcgac catactggcg aaacaatcgc tgaaacagat
    10141 gccccgccag cgcccagcac acattaccaa acgtcccaat cattgccagc aaaacgctga
    10201 cgccaactat ccagcttaac gcctgtgttt gcggcagaac aaatgtcgac agtgccgtaa
    10261 caccgtacaa aatgattttg acgttcacaa actgcaaagc aaagctggcc caaaaactga
    10321 ttggttttgt ctgaagcccg tcttcctttg ttggactggt agcgattttc cacgccagcc
    10381 agacaatata tgccgccccc gcccaactca acagatgcac agctgccggg tcgataacag
    10441 ccagtgaaaa cgaaatgccc gcacacagca acatgacgat taaaaatccc agactcatcc
    10501 ctgccagcac gcgggtactc tgatgaaatc cgtgcgttgt ggctgagcta agggcgagaa
    10561 tattgttcgg tcctggcgtc atggcggtaa tcagggtgta agtccaaaaa gcacttaaaa
    10621 gggtcggtgt cacttgttat ctcctctgtt
//
LOCUS       DBFIFM010000157        10648 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.157, whole genome shotgun sequence.
ACCESSION   DBFIFM010000157 DBFIFM010000000
VERSION     DBFIFM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.157"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(71..946)
                     /locus_tag="N7C74_003556"
     CDS             complement(71..946)
                     /locus_tag="N7C74_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HGU2433357.1"
                     /translation="MDFFTLFSPDGVMLETNVVCIVIALMFLYTFVGICAGFGGGLTT
                     MPLVTLMLPVKMATPLSVIVGTATALYATWLSRKETDWRSAMVLIIFSFLGIPVGLYA
                     LSYLPDHFMKVGLGGFLILYSFYSMFIPRLPIYDRSWIAAPIGAVAGALGAAFSTNGP
                     PMVIYGMLRNLGPAAFRGTLNAFFTANNIAVIGGLATSGILTVSIFKLVLFCIPTMIL
                     GSLVGQYVHKRISVKVFRVMVFLLLIASGSMLIKGALGIPAAAALLPPFTLLVILQLV
                     FGKKVAAIRQAEASK"
     gene            complement(1007..1909)
                     /gene="yihR"
                     /locus_tag="N7C74_003557"
     CDS             complement(1007..1909)
                     /gene="yihR"
                     /locus_tag="N7C74_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HGU2433358.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHGSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVTAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCSDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GLDLLLLEPGKTHRLFFNIYGQHN"
     gene            complement(1976..3217)
                     /gene="yihS"
                     /locus_tag="N7C74_003558"
     CDS             complement(1976..3217)
                     /gene="yihS"
                     /locus_tag="N7C74_003558"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HGU2433359.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDINAK"
     gene            complement(3233..4111)
                     /gene="yihT"
                     /locus_tag="N7C74_003559"
     CDS             complement(3233..4111)
                     /gene="yihT"
                     /locus_tag="N7C74_003559"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HGU2433360.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKTLSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(4135..5031)
                     /gene="yihU"
                     /locus_tag="N7C74_003560"
     CDS             complement(4135..5031)
                     /gene="yihU"
                     /locus_tag="N7C74_003560"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HGU2433361.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRSVLFGENGVCESLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFNMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            5199..6095
                     /gene="yihV"
                     /locus_tag="N7C74_003561"
     CDS             5199..6095
                     /gene="yihV"
                     /locus_tag="N7C74_003561"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HGU2433362.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYKQAKSSQSAIMVDAK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVVLATGGDLAESVRFASGVAASKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            6129..6914
                     /gene="csqR"
                     /locus_tag="N7C74_003562"
     CDS             6129..6914
                     /gene="csqR"
                     /locus_tag="N7C74_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGU2433363.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            7013..7612
                     /gene="yihX"
                     /locus_tag="N7C74_003563"
     CDS             7013..7612
                     /gene="yihX"
                     /locus_tag="N7C74_003563"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HGU2433364.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGTWSDLTRIPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            7606..8478
                     /gene="yihY"
                     /locus_tag="N7C74_003564"
     CDS             7606..8478
                     /gene="yihY"
                     /locus_tag="N7C74_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HGU2433365.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            8475..8912
                     /gene="dtd"
                     /locus_tag="N7C74_003565"
     CDS             8475..8912
                     /gene="dtd"
                     /locus_tag="N7C74_003565"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HGU2433366.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            8957..9898
                     /gene="fabY"
                     /locus_tag="N7C74_003566"
                     /gene_synonym="yiiD"
     CDS             8957..9898
                     /gene="fabY"
                     /locus_tag="N7C74_003566"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HGU2433367.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            10309..>10648
                     /locus_tag="N7C74_003567"
     CDS             10309..>10648
                     /locus_tag="N7C74_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433368.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNESPKQSLLNDFSHRLKMEG
                     EEQLFHCVNELKYGLSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGSRLLN
                     ARNQLDKNLLE"
BASE COUNT         2609 a         2615 c         2779 g         2645 t
ORIGIN      
        1 ccatcctgtt attacaggtt taattaatta aagaatgtgt gcggatataa atttaaaccc
       61 gcataagtga ttatttactt gcttcagcct gacggattgc cgccactttt ttaccaaata
      121 ccagttgcag tataactaac aaggtaaacg gaggtagaag tgcggcagcg gcgggaattc
      181 ccagcgcgcc ttttatcaac atggaaccgg aggcaatcag caataaaaag accatcacac
      241 ggaacacttt gactgaaata cgtttatgca catattgtcc aaccagtgaa ccgaggatca
      301 tggtgggaat acaaaacagc accagtttaa agatcgaaac agtcaaaatt ccgctggtcg
      361 ccagcccgcc gatcaccgcg atgttgtttg cggtgaagaa agcgttcaga gtgccacgaa
      421 atgccgccgg gcctaagtta cgcagcatac cgtagataac catcggtggt ccgttggttg
      481 agaaagctgc gcctaatgcg cctgctactg caccgatagg tgcggcgatc cagctacggt
      541 cataaatcgg caggcgtgga ataaacatac tgtaaaagga gtacaggata aggaatccgc
      601 ctaagcccac tttcataaaa tggtcgggta aatacgacag tgcatataaa ccgacgggta
      661 ttcccagaaa cgagaaaata atcagtacca ttgccgatcg ccaatccgtt tctttacgtg
      721 acagccaggt ggcgtacaag gcggtcgcgg tgccgacaat taccgagagt ggtgttgcca
      781 tttttacggg gagcattaac gtgacgagcg gcatagtggt taaaccaccg ccaaatcccg
      841 cgcatatccc cacaaaggta taaagaaaca ttaacgcgat aacgatacat accacgtttg
      901 tttccagcat gaccccatcc ggtgaaaata acgtaaaaaa gtccataata tttcctgttg
      961 tgtaaataca actctaaaca atgatctcgt tcttttctta ataaaattaa ttgtgttgcc
     1021 cgtaaatatt aaaaaacagg cggtgcgttt tccctggttc cagtaataat aagtcgagac
     1081 ctgaattaaa ggcgttaggc gggcaactca taggttcaac tgccagtccc tgacgctgta
     1141 atttttcacc gctataaatt tgtacccagg gttgatcgga acaaaggcta accgaaagcg
     1201 cctgttgtgg atgggtaatg gttatttccc acagatcatt agcggtttta aaggtgtggt
     1261 cgatttttgt cgcggctatt tttttcgcct gcgtgaaatt taaatccagc tcatcaacgt
     1321 ggtgaagcgt tgtcggatta gcgtgctcat cgacggcata aacctggttt gccggaagct
     1381 ggaacagata ttcatcaaca gatgttaagt tgcaggttaa ataaggatga atacccacac
     1441 catagggtgc ggtcacagta ccaatattct gacttgcgat ctcaacggaa agacccgtgc
     1501 gtgcattgag cgaataaaca acctgcgaag ccagcataaa cggatagccg taactgggcg
     1561 gcagaaaagc tgtcagggta acgctggtgg cggttaattc gctgatttgc cagtcacgcc
     1621 aggcgagcag cccatgaata gcggcttttg aaccatgttc attaatgggt aactgatatt
     1681 cctgcccctg atagcgataa cagccgttgg cgatccggtt cggccacgga ataaggactt
     1741 tgcccagatg tgccagcggc atctcttcgg gtttatgtgg aataactaaa tgacagccct
     1801 gaaaggttaa ttcagccaga cctgcaccga cggtcacaat ggtcgcgtga taatcccccg
     1861 cggcaagtga aacggtctgt ccactgcaat gcatgttggt gatttgcata cgaggtacct
     1921 ttattgaata acgacatacc cggcgtgcag cctgatatgt cgttttttta gtcagttatt
     1981 tcgcgttaat atccagcaga cccgccgcaa ctgccggagc caggcctggc gctaacggga
     2041 tacgcgggat caccaggcaa tgcagcaggt gataaatatc ctgtttgccg tcccagactt
     2101 tggtggtgac cttattgtcc gcatccagct cctgccacca tgaaccattt tcgtagtcca
     2161 tcaggtattt gatgcagtag tcccaccatg tctgatacca ggtttcgtac tggcgatcgc
     2221 cggtgacggt gtagagcgcg taggccgtac ccatcgcttc gacgataggc caacgcacac
     2281 gttcgcggac caccggtttt ccttcccagt caacggtata aacaatcccg tccgcaccat
     2341 cgggtgccca ggcatcacgc acggtggcgt taaacagacc tttggcatct tctagcagcc
     2401 atgctggcgg ttgttcgcaa cgggcttcca gggccgcgtg gatgtgcagc attaaacggc
     2461 cccattcgat ccagtggcct ggtgtacctc cgaacgcgcg gaagcggtgc gccgggttat
     2521 ctttgttgta atccggcagc ggattccact gggtatcgaa gtgttcgtta acgcgataat
     2581 gattatttct tgcgacgtcg tggataatca cggaagccac gcgaatcgcg cgatccagcc
     2641 attttttgtc atgcgttacg tcataaacaa ttaagaaagc ttccactgcg tgcatattgg
     2701 cattgccgcc gcggtactct tcggttttgc tgaaggcttc gtcccaggac tccaggcaca
     2761 tctgctcttc ttcgctccag aagtatttct cgataatttc aatggtgtaa tcgagcagct
     2821 tgcgcgcttc cgggtgaccc gttgtgacgg cgctggcagc acccagcaga gcaaagaaat
     2881 gttgatagcc ctgtttggag gcatccacca cgccctcgtc attcacacag gcataccagc
     2941 cgccatattt tttatcgcgc agtgcgccgt tcatggcttt aataccgtga tcaaccaacg
     3001 agtaagcgcc aggtcgaccc atcgccgcag caacggaata aacgtgcaac atacgagctg
     3061 tgatccacag atgggtgccc atctcttctt tgatttgccc tttattgcct aaccagccaa
     3121 atccggtcgg tactacggaa tttttaccaa aatcaaagat gcgatcggtt tcctgttcca
     3181 gccaacggtt gtgacttaaa gtgttaaacc atttcatttt tgtatcccca ttttaacggc
     3241 gtttagccat catttcgtcg acgatatcgc caagttgttg taatttcggt gcgcaaacgt
     3301 cacgcagcat cagctcgttg tctggtaaac cgacgaccga tgcccagaca gcacgaccag
     3361 ccaggaagcc cgatgcaccc gctgtcattg ccacgcgtac ggcacgcggg aacagttttt
     3421 cgtcgacacc ggaagagagg atcacccacg gcatattgat atggtcattc agacgttgtg
     3481 aagcactgag aagctcttgt tgcggacctt taccgtaaag cggcatttca actttgtaga
     3541 ggtcagcgcc actgtcgccc agctctttgg cggcatcgat gatcgcttgt tcgcgatcga
     3601 atttatcgcc acgacgtggt gggcggacga ctggctcaat aatgcttacc aggccgtgtg
     3661 agtggcacag ttcgttaaac tctttcacca tatccaggcg ttgctgcgca tcttcatcac
     3721 tacgccacag caccagcagt tttaaggctt tgccaccgtc ttgtttgatc tgtagcggat
     3781 tgattttgcg gtcaatgacc acgctatcta ccggaatacc gttgccagga atgaactcat
     3841 cggcggcgac aatcatggcg caacttttgg caatcgcgtt ttgctcaacc acctggcggt
     3901 aacagaattg ttgatctacc agaatcgccg aggcataagg cgagagggtc ttcgccgcgt
     3961 taactttgaa atcagttaaa acgctatcgg ctaccggagc gggtgcccca gccgcggcaa
     4021 acatcatgcg catggcttcg cgctgatcga ccgccagcat ggcaaaaccg cccgatgcgc
     4081 gcgtaatgtc gttgatggtg tacttattca ttcatttatt cctttatcca gtcgttacat
     4141 ttttactttg gcagtcatcc cggcactgac acggacctgt tccagaatgg cggaccagtc
     4201 ctggcgaccg cgacccgctg cgcgcgcctg gctataaacc tcccgtgagg cggcccccag
     4261 cggcattggc acatgcagct ggttggcgac atcaagggcg atgccaagat ccttatgggc
     4321 aagatcgatc atgaaggcgg gagaaagatc cccgctgagg actttgttcg gccaggaagt
     4381 ggtgaagtgg cctttaccgg cggcggtacc gctcatcact ttgacggcaa catcgaaggg
     4441 aagattcagg gcttcgcaca aaacggcagc ttctgccgaa agcgcattga gcgcgatgct
     4501 catatagttg ttgatgagct taacgcggat ccccatgccc ggaccgcctg cgttgatcaa
     4561 ctcactgccc atcgccatca ggatcggcgt ggcacgttca acttgttcag cggtgccgcc
     4621 agccagcagt aacagagtac cggtaatggc atttgcagaa gtacggccta ccggaacatc
     4681 catcatgttg aagcctttgg cttgcatatc ggcaatcaat ttatcggttt gcagcggatg
     4741 gatggtcgac atatcaatga ccagcgcatc ggtagataag ctttcgcaaa cgccgttttc
     4801 accgaacaac acgctgcgca ccagatcgcc attcggcaac atggtaatga taaattcggc
     4861 atctttagcg gcctgcgccg ggttggcggc gggagtcgcg cctttgtcta ccagatgccg
     4921 cacagcctcg gcattcacat caaagacgcg aagttggtgc ccttgctgca ataaattgct
     4981 cgccattggc gaacccattt gtcctaaacc gataaacgcg attgctgcca taaccttctc
     5041 ctgaatacag tgatgtcact ttttgtcatt tatgacacgc tttgattgtc tgtttttgat
     5101 cgtatttgta atttatagtc aaaaaattga cagacgtcac tttttaaaca attggtgaaa
     5161 ttaaaatgaa cgcatcccaa aatgtttaag gaatgaccat gattcgtgtt gcttgtgtag
     5221 gtataaccgt gatggatcgc atctattacg tggaagggtt accgacggag agcggtaaat
     5281 acgtggcgag aaattatacg gaagttggtg gcgggccagc ggcgacggca gcggttgcgg
     5341 cggcaagact gggagcgcag gttgatttta ttgggcgcgt cggtgatgac gacaccggca
     5401 acagcctgct ggcagagctg gaatcctggg gcgttaacac ccgctacacc aaacggtata
     5461 aacaggcgaa atcgtcgcaa tccgccatca tggtggatgc caaaggcgag cggataatca
     5521 ttaactaccc cagcccggac ctgctgcctg acgcagagtg gttggaggaa attgatttct
     5581 ctcagtggga tgttgtgctg gcagatgtac gctggcacga cggcgctaaa aaagccttca
     5641 ctctggcccg gcaggcgggc gtgatgaccg ttctggacgg tgatattacg ccgcaggata
     5701 tcagcgagct ggtggcatta agcgatcatg cggccttttc agaaccgggt ctggcgcgct
     5761 taacgggcgt gaaagagatg gccagtgcgc taaaacaggc acaaacgctc acaaatggtc
     5821 atgtctatgt gacccagggc agcgcaggct gtgactggct ggaaaatggc gggcgtcaac
     5881 atcaaccggc cttcaaagtt gatgtggtag ataccacagg tgcgggtgat gtttttcacg
     5941 gcgcattggc ggtggtgctg gcaacaggtg gggatttagc ggagtcagtc cgcttcgcca
     6001 gcggtgtagc ggcgtcgaaa tgcacgcgtc ccggtggacg agccgggatc cctgactgtg
     6061 atcaaacccg atcttttttg tcactttttg tataaaatgc cagggtgatg gtttttcgag
     6121 gaattttcat gagccttacc gaactaaccg gtaacccgcg gcacgaccaa ctcctcatgc
     6181 tgatcgccga gcgtgggtat atgaatattg atgagctggc aaatctgctg gatgtctcca
     6241 cgcagacggt gcgccgggat attcgtaaat taagcgagca aggcctgatt acgcgccatc
     6301 acggtggcgc gggtcgggct tccagcgtcg ttaatacggc gttcgagcag cgtgaggttt
     6361 cgcaaaccga ggaaaaaaaa gcgattgccg aagcggtggc agactatatt cctgatggtt
     6421 caacaatatt tatcaccatt ggtacgaccg ttgagcatgt tgcccgggcg ttacttaacc
     6481 ataatcattt gcggataatc accaacagcc tgcgtgtggc gcatattctt taccacaacc
     6541 cgcgctttga agtgatggta cccggcggta cgttgcgctc tcataatagc gggatcattg
     6601 gcccttcagc ggcgtccttt gtggccgatt ttcgtgctga ttatctggta acaagcgttg
     6661 gggcgattga gagcgatggc gcgttgatgg agtttgatgt aaacgaagct aacgtggtga
     6721 aaacgatgat ggcgcacgcg agaaatatcc tgctggttgc cgatcacact aagtatcatg
     6781 cttcggcggc ggttgaaatt ggtaacgtgg cacaggtcac cgcgctcttt accgacgagc
     6841 tgccgcccgc tgcgctaaaa tcacgcttac aagacagcca aattgaaatc atccttcccc
     6901 aggaagacgc gtagattttt gtgactgttg acctggcttc ataccttgcc acatagccaa
     6961 acccatcctt tcccgctaca gttaatttct tgtggcgcga aaggaggcaa aaatgctcta
     7021 tatctttgat ttaggtaatg tgattgtcga tatcgacttt aaccgtgtgc tgggaacctg
     7081 gagcgattta acgcgtattc cgctggctac gcttaagaag agttttcata tgggggaggc
     7141 gtttcatcag catgagcgtg gggaaattag cgacgaagcg ttcgcagagg cgctgtgtca
     7201 tgagatggct ctaccgctaa gctacgagca gttctctcac ggctggcagg cggtgtttgt
     7261 tgcgctgcgc ccggaagtga tcgccatcat gcataaactg cgtgagcagg ggcatcgcgt
     7321 ggtggtgctt tccaatacta accgcctgca taccaccttc tggccggaag aatacccgga
     7381 aattcgtgat gctgctgacc atatctatct gtcgcaagat ctggggatgc gcaaacctga
     7441 agctcgaatt taccagcatg ttttgcaggc ggaaggtttt tcacccagcg atacggtctt
     7501 tttcgacgat aacgccgata atatagaagg agctaatcag ctgggcatta ccagtattct
     7561 ggtgaaagat aaaaccacca tcccggacta tttcgcgaag gtgttatgct aaaaaccatt
     7621 caggacaaag ccaggcaccg tacccgtcca ctatgggcct ggctaaaact actctggcaa
     7681 cgcattgatg aggacaatat gacaaccctg gcaggtaacc ttgcctatgt gtcgttgctc
     7741 tcattagtgc cgctggttgc cgttgttttt gcgcttttcg ccgcttttcc catgttttcc
     7801 gacgtcagca ttcagttacg tcactttatt tttgccaact ttctgcctgc tactggcgat
     7861 gttattcagc ggtatatcga acaatttgtt gccaattcca acaagatgac cgccgttggg
     7921 gcgtgcgggc tgatcgtcac cgcgttattg ttgatgtact ccatcgatag cgcgttgaat
     7981 accatctggc gcagtaaacg agcgcgaccc aaaatttact cgtttgccgt gtactggatg
     8041 attttaacgc tggggccgct gctggcaggg gccagtctgg cgatcagttc ctatttgctc
     8101 tccctgcgct gggcgagcga tctcaatact gtcatcgaca acgtgctgcg tatttttccg
     8161 ttgctgttgt cgtggatctc cttctggttg ctgtacagca ttgttcctac catccgcgta
     8221 cctaaccgcg acgcgattgt cggcgcgttt gttgctgcac tcctgttcga agcaggaaag
     8281 aaaggtttcg cgctttatat caccatgttc ccgtcatatc agctcattta cggtgtgctg
     8341 gcggtgatcc ccattctctt tgtttgggtc tactggacat ggtgtatcgt cttgcttggc
     8401 gcggaaatta ctgtcactct cggggaatac cgcaaactaa aacaagcagc tgaacaagaa
     8461 gaagacgacg aaccatgatt gcattaattc aacgcgtaac ccgtgccagc gtcaccgtgg
     8521 agggagaagt gacgggcgaa attggtgcgg gacttttggt gttattgggt gtcgaaaagg
     8581 atgacgacga acagaaagca aaccgtctgt gcgagcgtgt gctcggctac cgcatcttta
     8641 gcgatgccga aggcaagatg aatctcaacg tgcaacaggc gggcggcagt gtgctggtgg
     8701 tttcccagtt taccctcgcc gcagataccg aacgggggat gcgcccaagt ttctccaaag
     8761 gtgcatcacc ggatcgcgca gaggcgttat atgactattt cgtcgaacgc tgccgtcagc
     8821 aagagatgaa cacgcaaaca ggacgcttcg ctgcggatat gcaggtatcg ctggtcaatg
     8881 atggccccgt gacattctgg ttgcaggtat gagccagctt ccagggttgt cacgggaaac
     8941 aagagagagt atcgctatgt atcaccttcg ggttccacaa acagaagaag aattagagcg
     9001 ttactatcag tttcgctggg aaatgttgcg taagcccctg catcaaccaa aaggttcgga
     9061 acgcgacgcg tgggatgcga tggcgcatca ccagatggtc gtcgacgagc agggtaatct
     9121 ggtggcggta ggccgactgt atattaatgc cgacaatgaa gcgtccattc gctttatggc
     9181 cgttcatccc gacgtgcagg acaaagggtt aggcacgctg atggcgatga ccctggagtc
     9241 ggtggcgcgt caggaaggcg ttaagcgcgt gacctgtagc gcccgtgaag acgcggtgga
     9301 gtttttcgcc aagctggggt ttgttaatca gggagaaatc accacgccaa ccaccacgcc
     9361 gattcgccat tttttgatga ttaagcccgt cgccactctg gatgatattt tgcatcgcgg
     9421 cgactggtgc gcgcagctgc aacaggcgtg gtacgaacat atcccgctta gtgaaaaaat
     9481 gggcgtgcgc attcagcaat ataccgggca aaaatttatc actaccatgc cagaaaccgg
     9541 caatcagaat ccgcaccata cgctgtttgc cgggagttta ttctcactgg cgacgctcac
     9601 cggttggggg cttatctggc tgatgctgcg cgaacgccac ctcggcggaa cgattattct
     9661 ggcggatgcg catatccgct acagcaaacc gattagcggt aaaccccatg cggtagccga
     9721 ccttggtgcc ttaagcggcg atctcgaccg tctggcgcgc ggacgaaaag cacgggtgca
     9781 gatgcaggtc gaaatctttg gcgacgagac gccgggtgca gtgtttgaag gcacgtatat
     9841 cgttctgccc gcgaagccat ttggcccgta tgaagagggc gggaacgaag aagagtagct
     9901 gatggagagg ggcggtgctg cctctctcat tcaggtcatt tatattagtc cttggctatt
     9961 tcggttatat tgaataagaa ctctataaat ttttattctt cgttggctcc atacgtgtct
    10021 tgctacatca ctgttcagga gcatttaaat attatttgta gagctgtata gcaattatcg
    10081 cagataatgc ttaattcatc atcttagttc aggtttgagc gatttttgat agtcaaaacc
    10141 ccctctggtg ccatagtttt ctcataaata ctttaaaaat ctatagctaa caaacattat
    10201 gcaaagataa tgggctggca tcattgtcag tatcattcat ttactgctct ggatgttata
    10261 aataattatg tagcatgttt gctcttaaac cgtgggaagg aggctgtaat ggttgccaat
    10321 attaatctca ttaaacaaga aagttattct gttgttaatt tagaaaaaca actaatgagt
    10381 aatgaatcac cgaagcaatc gctattaaat gatttttcac atcgccttaa aatggaaggt
    10441 gaggaacaac tgttccattg tgttaacgaa ctgaagtatg gtttgtcgag taataatgaa
    10501 tatgacctgt ttaaaaatag agaaacctca ctgtggaaac gcaaagtacc agaaagcgca
    10561 gcgctggcaa tgacgggaac agtatataaa ttatttggta gtcgcttact caacgcccgc
    10621 aatcagttag ataaaaatct cctcgaaa
//
LOCUS       DBFIFM010000158        10617 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.158, whole genome shotgun sequence.
ACCESSION   DBFIFM010000158 DBFIFM010000000
VERSION     DBFIFM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.158"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            114..680
                     /gene="yceJ"
                     /locus_tag="N7C74_003568"
     CDS             114..680
                     /gene="yceJ"
                     /locus_tag="N7C74_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HGU2433369.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            684..1259
                     /gene="yceI"
                     /locus_tag="N7C74_003569"
     CDS             684..1259
                     /gene="yceI"
                     /locus_tag="N7C74_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HGU2433370.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1301..2353)
                     /gene="trhO"
                     /locus_tag="N7C74_003570"
     CDS             complement(1301..2353)
                     /gene="trhO"
                     /locus_tag="N7C74_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HGU2433371.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVKTFRAQLYAFDSALDGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2578..3498
                     /gene="lpxL"
                     /locus_tag="N7C74_003571"
     CDS             2578..3498
                     /gene="lpxL"
                     /locus_tag="N7C74_003571"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HGU2433372.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3670..4896
                     /gene="mdtG"
                     /locus_tag="N7C74_003572"
                     /pseudo
     CDS             3670..4896
                     /gene="mdtG"
                     /locus_tag="N7C74_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            4979..5353
                     /gene="msyB"
                     /locus_tag="N7C74_003573"
     CDS             4979..5353
                     /gene="msyB"
                     /locus_tag="N7C74_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HGU2433373.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5354..5581)
                     /gene="yceK"
                     /locus_tag="N7C74_003574"
     CDS             complement(5354..5581)
                     /gene="yceK"
                     /locus_tag="N7C74_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HGU2433374.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5754..8297)
                     /gene="mdoH"
                     /locus_tag="N7C74_003575"
     CDS             complement(5754..8297)
                     /gene="mdoH"
                     /locus_tag="N7C74_003575"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HGU2433375.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8290..9825)
                     /gene="mdoG"
                     /locus_tag="N7C74_003576"
     CDS             complement(8290..9825)
                     /gene="mdoG"
                     /locus_tag="N7C74_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HGU2433376.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10218..>10617
                     /locus_tag="N7C74_003577"
     CDS             10218..>10617
                     /locus_tag="N7C74_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HGU2433377.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLA"
BASE COUNT         2622 a         2754 c         2601 g         2640 t
ORIGIN      
        1 attcaaaatt attgaaataa aaataaatat cattcaattt gttatttttg tgttgcaatt
       61 cattctccga cacgccattt tctttctctc acaaattcgt tttggatgca cttatgtcat
      121 tcacaaatac ccctgaacgc tatggtgtta tatctgcggc cttccattgg ctaagcgcaa
      181 ttattgtcta cggcatgttt gctttaggtc tgtggatggt cacgctcagt tattacgatg
      241 gctggtatca caaagcaccc gaactacata aaagcatcgg tattttgtta atgatggggc
      301 tggttattcg cgttctgtgg cgtgtcatat ctccaccgcc gggtccgctg ccgagttatt
      361 cgcccatgac tcgtcttgcc gcaaaagctg gacatctcgc attgtaccta ttgctgttcg
      421 ccatcggcat cagcggctat ctgatctcaa ctgccgatgg taaaccgatc agcgtttttg
      481 gctggtttga cgtccccgcg acccttgccg acgctggcgc acaggcagac tttgccggtg
      541 ccctgcattt ttggctcgcg tggagcgtcg tagtactgtc cgttatgcac ggatttatgg
      601 ccctgaagca tcatttcatc gataaagacg acactctgaa gcgcatgctg ggaaagtcgt
      661 catctgacta tggagtataa aaaatgaaaa aaagcctgct tggtttaacc ttcgcgtccc
      721 tgatgttctc tgccggttca gcggttgccg ccgattacaa aattgacaaa gaaggtcagc
      781 acgcctttgt taactttcgc atccagcatc ttggctatag ctggttatat ggcaccttta
      841 aagatttcga cggtactttt acctttgatg aaaaaaatcc agctgccgat aaagtgaatg
      901 tgacaattaa caccaccagc gtcgatacta atcacgccga acgtgataaa catcttcgca
      961 gtgcagattt cctcaatacc gcaaaatatc cacaggcaac attcacctcc accagcgtga
     1021 agaaagacgg tgacgaactg gatattaccg gcgatctgac gctgaatggt gtaaccaaac
     1081 ccgtcacgct ggaagcgaaa ttaattggtc agggcgacga cccatggggt ggtaaacgtg
     1141 caggcttcga ggccgaaggc aaaattaagc tcaaggactt caatatcaag acagatttag
     1201 gtccagcttc tcaggaagtg gatctgatta tttcagtgga aggcgtacag cagaagtaag
     1261 agtgaaatgt tgccggatgg cacgcatccg gcaatgatat ttattctgtt ggatcaggaa
     1321 tgcccagtgt tgtattcaga cgtccacgag acttattaaa gatcttattg ccattttcac
     1381 gtcctgcccg acggcgtcgc tgttcttctg gcggtaacgc gctttcttcg cagcaaattt
     1441 cactacaaca acctttgtat ttttccgcac atactggaca ctgaataaac agcaggtggc
     1501 agccatcatt tttacagttg gtatggctgt cgcacggcgc accgcactgg tggcaatgcg
     1561 cgataatctc atcagatata cgttcgccca tccgctcgtc aaaaacaaaa tttttgccaa
     1621 taaaacgcac cggcaagccc tgctcgcgcg ccttacgggc gtattcaata attccaccct
     1681 cgatatgcca gactttattg aatccgttat gtttcatcca ggcactggcc ttttcacaac
     1741 gaatgccgcc ggtgcagtac atgacgattt ttttatcttt atgtgcctgc atcatctcga
     1801 ctgcttttgg cagctgctca cggaaggtat ctgccggaat ttccagcgcg ttttcaaagt
     1861 gccccacttc atactcatag tggttacgca tgtcgataaa cagtgcatcg ggatcgtcaa
     1921 gcatggcgtt cacttccgcc gcttgcagat actcaccaac attgctggca tcaaagtgag
     1981 gatcgtcaat accgtcggca acgatacgat cgcggacctt catgcgcagt acccagaagg
     2041 atttcccgtc atcatccaac gcgatattca ggcgtaagcc atctaaagcc gagtcgaagg
     2101 catagagctg cgcgcgaaat gttttaacat tgctcgcagg tacgctgatt tgcgcgttaa
     2161 tgccctcatg cgccagatac actcgcccaa aaacattcag cgcggtaaac agctgatata
     2221 aagcgtcacg ggtcgcctta ggatcggcga tgtggaaata cttgtaaaac gaaatggtgg
     2281 ttcgcggttc gctctcagcc aacattttgg cttttaacgc gtcgttggaa atgcggttgt
     2341 gtaacactgg catggtgtac ggttcctgcg agatgggaaa gtaaaaatcc gcggcatgat
     2401 atagcaatta tcgataatta acatccacac attttacgct acatttgcgc attaaaaatt
     2461 atttgttatt tacaagcgcg gcaatttcgc ccagtcttca gccacaaatt ttggttgcgg
     2521 gcgaaaaaat gcgacaatac atacaattgc ccgaataggt tgaaaaacag gattgatatg
     2581 acgaatctac ccaagttctc caccgcactg cttcatccgc gttattggtt aacctggttg
     2641 ggtattggcg tactttggtt agtcgtgcaa ttgccctacc cggttatcta ccgcctcggt
     2701 tgtggattag gaaaactggc gttacgtttt atgaaacgac gcgcaaaaat tgtgcatcgc
     2761 aacctggaac tgtgcttccc ggaaatgagc gaacaagaac gccgtaaaat ggtggtgaag
     2821 aatttcgaat ccgttggcat gggactgatg gaaactggca tggcgtggtt ctggccggac
     2881 cgccgaatcg ctcgctggac ggaagtgatc ggcatggaac acattcgtga cgtgcaggcg
     2941 caaaaacgcg gcatcctgtt agttggcatc cattttctga cactggagct gggtgcgcgg
     3001 cagtttggta tgcaggaacc ggggattggc gtttaccgcc cgaacgataa tccactgatt
     3061 gactggctac aaacctgggg ccgtttgcgc tcaaataaat cgatgctcga ccgcaaagat
     3121 ttaaaaggca tgattaaagc cctgaaaaaa ggcgaagtgg tctggtacgc accggatcat
     3181 gattacggcc cgcgctcaag cgttttcgtc cctttatttg ccgttgagca ggctgcgacc
     3241 acgaccggaa cctggatgct ggcacggatg tccggcgcat gtctggtgcc cttcgttcca
     3301 cgccgtaagc cagatggcaa agggtatcaa ttgattatgc tgccgccaga gtgttctccg
     3361 ccgctggatg atgccgaaac taccgccgcg tggatgaaca aagtggtcga aaaatgcatc
     3421 atgatggcac cagagcagta tatgtggtta caccgtcgct ttaaaacacg cccggaaggc
     3481 gttccttcac gctattaaat ctcccatgcc ggatgcttca gaatggcatc cggcattacc
     3541 acagcaaatc cccctgattt agcgataaaa gctctctgga ttgcgccccc tggaagtcgg
     3601 gcgcataatt agtgtgctta tcttttcttc ttatgttcac cgcgcctggc gcaccaacag
     3661 cggattgcta tgtcaccctg tgaaaatgac acccctataa actggaaacg aaacctgatc
     3721 gtcgcctggc taggctgttt tcttaccggt gccgccttca gtctggtaat gcccttctaa
     3781 cccctctacg ttgagcagct tggcgttacc ggccactccg ccctgaatat gtggtccggt
     3841 attgtcttca gcattacatt tttattttcg gccatcgcct caccgttttg gggtggactc
     3901 gccgaccgta aaggccgaaa actcatgcta ttacgctctg ctctcggcat gggcatcgtg
     3961 atggtgttga tggggctggc acaaaatatc tggcagtttt tgatcctgcg ggcgcttctt
     4021 gggttacttg gcggatttgt ccccaacgct aatgctctta tcgccacaca agtaccgcgt
     4081 aataaaagcg gctgggcact gggtacgctc tccacaggcg gcgttagcgg tgcgttgctc
     4141 ggcccaatgg ctggcggcct gctcgccgat agctacggct tacgtccggt attctttatt
     4201 accgccagtg tgctcatact ctgctttttc gtcaccctgt tttgcatcag agaaaaattc
     4261 cagccggtca gcaaaaaaga gatgctgcac atgcgggaag tggtgacatc acttaaaaac
     4321 ccgaaactgg tactcagcct gtttgtcacc acgttaatca tccaggtggc gacgggctca
     4381 attgccccca ttctgacgct gtatgtccgc gaactggcgg gtaacgtcag taacgtcgcc
     4441 tttatcagtg gcatgatcgc ctcggtgcca ggcgtagctg ctctgctgag tgcaccacga
     4501 ctcggcaaac ttggcgatcg aatcggaccc gaaaagatcc tgattacggc gctgatcttt
     4561 tctgtactgc tgttgatccc aatgtcttac gttcagacgc cattgcaact tgggatttta
     4621 cgttttttgc tcggtgccgc cgatggtgca ctactccccg ccgtacagac actgttggtt
     4681 tacaactcga gcaaccaaat cgccggacgt atcttcagct ataaccaatc gtttcgtgat
     4741 attggcaacg ttaccggacc attgatggga gcagcgattt cagcgaacta cggtttcaga
     4801 gcggtatttc tcgtcaccgc tggcgtagtg ttattcaacg cagtctattc atggaacagt
     4861 ctacgtcgtc gtcgaatacc ccaggtatcg aactgatttt tcgcctttca tacttgcaaa
     4921 agcggagaat cagctatcct tttccctgaa acctcatcaa ctcaaaggga gaatcgtgat
     4981 gaccatgtac gcaacgcttg aagaagccat tgacgctgca cgcgaagaat ttcttgcaga
     5041 caaccccggc atcgacgccg aagatgcgaa tgtgcaacag ttcaatgccc aaaaatacgt
     5101 tttgcaggac ggcgacatca tgtggcaagt tgagtttttt gccgacgaag gggaagaagg
     5161 tgaatgttta cctatgctta gcggtgaagc cgcgcaaagt gtttttgatg gcgactatga
     5221 tgagatagag atccgccagg agtggcagga agagaataca ttacatgaat gggacgaggg
     5281 ggaatttcag cttgagccac cgctggatac cgaggaagga cgcgcagcag ctgatgagtg
     5341 ggatgaacgt taattactca tacgggccat gatgaatgtc gatcggcagc agtaaagtat
     5401 caaaaaccag ggagaatggc agatcaagga ttgtgacata acgccaggcg gagtcacgca
     5461 catcccattg cacaccggga taatattggt tgccatgccc ctgccccggt atagtgcggc
     5521 taatgatgct gccacagcca ctcagcaagg tcaccatgat gctcaccaca attaaacgca
     5581 tttgatacct cttccgtaaa aaaataccgg cataacgccg gtatttttat tggctcgttg
     5641 tttttgccag atgtggcgtg aacgccttat ccgacctaca aaatcttgca aaatcaacac
     5701 attgcaggag cgatgtaggc ctgataagca tagcgcatca ggcaactacg tttttattgc
     5761 gaagccgcat ccggtttacg caatgccagt ggattgagct ttatcccttc gtaataactc
     5821 acccatgaag aatatctctc aggggaattc cagacgcgga aatgcagacg ggccatcgtc
     5881 accggatcgc ttagcagcac aaggcgacga tcgcgattca gcttctctgg cgtctcgttc
     5941 agcgcctgtt caacatggcg gtcacgggcg atttccagca ccttactggc gcggtgacgc
     6001 gcggtcgcca ttgcggttgc cagagcgtta aatgacgggt taaacactgc gtgcataaaa
     6061 ccatcatcaa gggagcgttg acggttcatc tcaaggaacc gatcggtatc aaccagtacc
     6121 tgcggcggtg aatactcttc cgggatcagg aacagtttcc agcgtttggt gcgcagacca
     6181 acggtggcac ggctggaaat caccgaaaca aacggtgaca ggatcaacga gaagacaatc
     6241 ggtgccagcc agaacaggaa acgcagatcc aaccacgcca tcccgacagc ccacactaac
     6301 cctaacagca gctgtgagcc gtggcgtttg aacgcttcac cccaggaagt ggagtcatca
     6361 tcacgctgcg gtgaattcca caccacttcc cagccaagga acgcgctgac gacgaagacc
     6421 gtatggaaca gcatgcgtac cggagccagc agcacggaaa acagcacttc cagcagcaac
     6481 gataatgtaa cgcgccagaa gccgccatat tctttcgttc ctttacacca aataagcaaa
     6541 atgctcaata gctttggcag gaacaacagc accatggtcg aagcaaaaag tgcaatcgcc
     6601 agctcaggac gccactgcgg ccacaccggg aacaactgcc gtggttgcag gaagtattgc
     6661 ggttcggtca acgcatgcac tacctgcaat gcagtagaga gcgcgaggaa cataaaccac
     6721 agcggagcgg agagataaga catcacgccc gtcaggaata ccgcacggtg aaccgggtgc
     6781 atacccttca ccaggaacag acggaagttc atcaggttac cgtggcacca gcggcggtca
     6841 cgttttagct catcaagcaa gttaggcggt aattcttcat aagaacccgg gagatcgtaa
     6901 gcaatccaga ctccccaacc tgcacggcgc atcaacgccg cttccacgaa gtcatgtgac
     6961 aggattgaac cggcaaagga accttcgccc ggcagcgggg ccagtgcaca gtgctcgata
     7021 aacggtttca cgcggataat cgcgttatgc ccccagtagt gcgactcgcc aagttgccag
     7081 aagtgcaaac cggctgtaaa cagtggccca tacacgcggg tcgcgaactg ctgacagcgt
     7141 gcatacagcg tatccatacc ggacgctttc ggcgacgact gaatgatccc ggcgttcggg
     7201 ttggcttcca tcaggcgcac caggccgcac aaacaatcac cggtcattac cgagtcagca
     7261 tccagcacca ccatatagct gtactggctg ccccagcgac ggcagaagtc atcgatatta
     7321 ccgcttttac gcttcacgcg acggcggcgg cggcgataga aaatctgacc ttcgccaccg
     7381 acttcagcga taagctccat ccaggctttt tgctctgcga cgcagatatc cgggttgtaa
     7441 ctgtcactca gaatgtagac atcaaagtgt ttggcattcc cggtggcttt tactgattcc
     7501 cacgttgcac gcaagccagc aaaaacacgg ttcacgtctt cgttacagat aggcatgatc
     7561 aacgccgtgc gatgctccgg gtttaatggt tcatcgccaa ctgttgacgc agatatactg
     7621 tatttatcgc gaccaataag tagttgcagg aagcccatta acgccgtcca gaatccggcg
     7681 gacacccaac agaacagtac cgcaaagagg atcaggatac cggtttgcag catataaggc
     7741 agaagctgca taaaggaaac ccacaaatcc tgaccaacca tatccatagg attaatcagc
     7801 gcccaaccct gataaggaag aatggtcttc atataccagg tcgcgacgac agtttgcgcg
     7861 agcgtcagga tcaacagaat gtaacggcgg atggtaccga cggtacgcca cttttgctcg
     7921 ctctcctgct cttctttggt caaacgagcc agatagcgcg gtgttacatc gcgtccacgc
     7981 aggcgatccc agaaacggcc taccgggttg gtacgccacg ggtcgggaaa catcgaggag
     8041 cgttttgctt ctggcatcgc cttaagctga tcgcgccctt cgtcatcttt aattaactgt
     8101 ccatcagcaa gtgaatctgg ccaggcttgt tccagacgcg cctttaccga gccttgcggg
     8161 gagtcatcct cccgcgccca ggtgcggtgt tcggcatcca gcgcctgatg aacggcgcgg
     8221 atatcagtct tcggcaatgc cgctttctcg cttgcggcga tgggcattgc gtcaatgtac
     8281 tcagttgtct tattcattgg caggtaactg gtagctccag gtttcactca acgtctgatc
     8341 ggcattcacc agcgcagcgc gcatttcagt ggttttcttg gcatctttca ctttcacacg
     8401 catcaccaga cgccagcctt tggtgaccgg gttatagcgc accgtgcttt caactatctc
     8461 accattatca ccaatgctgg tttgcgctgt gaccggggta tcctctggca gttttttcat
     8521 ctctgcgccg gtaaaatcga ccacaaaggc gatagtaccg tcaggctggc gaatcaggtt
     8581 cgactgcttc acatcccccg ttgaacgacg cgtttgttgc acccatgcgt tatctggcgc
     8641 atgcagtttg tcttcatcac ggctgaaggt gatggtgtat ttaaagttca tctctttacc
     8701 cggctccggc agctgatccg gcgtccagta agcgacgatg ttatcgttag tttcatcgtt
     8761 ggttggaatt tccaccagct caacgctgcc tttaccccac tcccctttcg gagtcaccca
     8821 tgcgcttgga cggagatcgt aacgatcatc gagatcttca aagcgggaga aatcacgacc
     8881 gcgctgcaac agaccaaagc cttgcgggtt ttccatggag aagctgctga ccgctaaatg
     8941 tttcgggtta ttcaacggac gccagatcca ttcgccatta ccggcatgga tagagagacc
     9001 gttagagtcg tgcaactccg gacgatagtt atttgctggc gacggttggt tcggcccaaa
     9061 caggaacata ctggttaacg gtgcaacccc cagtttgccg actttatcgc gcagatagat
     9121 tttcgactgc acatccacaa ccgtgtcacg ccctggcata actacgaatt tgtaagcacc
     9181 tgtcgcgcgc ggcgagtcaa gcaatgcata aatggttaaa cgtttatcag ttggttttgg
     9241 acgctcgatc cagaactctt tgaagcgtgg aaattcttca cccgatggca aggcggtatc
     9301 aattgccaga ccgcgggcag aaaggccata aacctgacct gcaccaatca cgcggaaata
     9361 gctggccccg agcatgctga cgatttcatc gtttttatct ttgctgttga tcgggtaaag
     9421 cactttaaaa ccggcaaaac caaggtcttt taccgtgtct ttgtcatgct gaacatcgcc
     9481 gaaagtgaaa taatccgggc tgtatttgat tcgtttgact gcggtggcag tcacttcatt
     9541 tattttgacc ggggtatcga agtacatacc ctgatggtag aactcgagtt tgaatggggt
     9601 cttcagattg ttccagtacg ctttgtcatg attaaactgg atctgctgat agtccgcgta
     9661 tttcatatcg cggaaaacgg agggcaagtt gcttttgggc gcctcatagc ctttcccggc
     9721 taaggattga gcttgctttg cgacatcatc aatactgaaa gcccagcttg aagatgtata
     9781 cagggttaac attactgcag cactcaacca acgcattttc atcatttgta gtttatgttt
     9841 cataattagt aagcacttcc ccctttgtgt gcttatatcg atccgatcta ttttaatgga
     9901 taaccagcca ttccgacaac agaatcccgg tatcgttcag cacgctggct catggattaa
     9961 gcacctgatt gcaaccgttt tcactttccg tgcttctcct aaagacagaa gcatcgactt
    10021 cgtgtagggt ttgccagcaa atttatttat caaccgtctt acagacgctt ccattctggt
    10081 tagtggtagc gatgatgcta tgcataaaaa cttgaagcct gcgcctggcg cggtcaggtc
    10141 ggtgtcgcta tggcaacaag cctaaaggca taacccgaca gggataagac gaaaaatccg
    10201 agattagtta accatatatg aatccagtac ccgcgcaacg tgaatatttc ctcgactcca
    10261 tccgcgcctg gctgatgttg ttagggatcc cttttcatat ttctttaatc tattcgagcc
    10321 atacatggca tgtgaatagc gccgaaccgt cattgtggct gacccttttt aatgacttca
    10381 tccactcgtt ccgcatgcag gtatttttcg ttatatccgg gtacttttcc tacatgcttt
    10441 ttttacgcta tcccttgaaa aaatggtgga aagtacgtgt cgaacgtgta gggatcccga
    10501 tgttaacagc catcccccta ctgacattgc cgcaatttat tatgctgcaa tacgtcaaag
    10561 ggaaagcgga aagttggcct gggctgtcat tgtatgacaa atataatacg ttggcct
//
LOCUS       DBFIFM010000159        10614 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.159, whole genome shotgun sequence.
ACCESSION   DBFIFM010000159 DBFIFM010000000
VERSION     DBFIFM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.159"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            91..417
                     /locus_tag="N7C74_003578"
     CDS             91..417
                     /locus_tag="N7C74_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU2433378.1"
                     /translation="MATAGIVVGGTRVIFYGDKPDTTISIFNKETNLPYLLQTWVDPF
                     NKEDKSKPPFSVIPPVSRIEPQQEKILRILKTAGELPKDRESVFWLNVKNIPPSIDKK
                     IPMFLR"
     gene            570..719
                     /locus_tag="N7C74_003579"
     CDS             570..719
                     /locus_tag="N7C74_003579"
                     /inference="COORDINATES: protein motif:HMM:NF014773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2433379.1"
                     /translation="MDDKDVSLNMIRPFEILTLPIPAGVAGKSLVWRFINDYGAISQP
                     LKKNL"
     gene            741..3275
                     /locus_tag="N7C74_003580"
     CDS             741..3275
                     /locus_tag="N7C74_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU2433380.1"
                     /translation="MASSHLFITLASGICLLCSISAFARDSLFNPRLLELDHPADNID
                     IHQFNRSNTLPAGTYKVDVMINGMLFERQEVKFAQDNPDAELHPCYVAIKNVLATYGI
                     KVDAIKSLANVDDKTCVNPVPLIDGATWLLDASKLALNITIPQIYLNNAVNGYISPSR
                     WDQGINAMMMNYDFSASHTIRSNYDDDDDSYYLNLRNGINLGAWRFRNYSTLNSYDGN
                     VDYHSVSNYIQRDIMALRSQIMIGDTWTASDVFDSTQVRGVRLYTDDDMLPSSQNGFA
                     PVVHGIAKTNATVIIKQNGYVIYQSAVPQGAFALTDLNTTSSGGDLDVTIKEEDGSEQ
                     HFIQPFTSLAILKREGQTDVDLSIGEVRDESGFTPEVLQLQAMHGFPLGITLYGGTQL
                     ANDYASAALGIGKDMGALGAISFDVTHARSQFDYDDNESGQSYRFLYSKRFEDTNTTF
                     RLVGYRYSMEGFYTLNEWVSRQDNDSDFWVTGNRRSRFEGTWTQSFTPGWGNIYLTFS
                     RQEYWQTDEVERLLQFGYNNNWRNISWNVSWNYTDSIKRSLGNHHDDNNDDFGKEQIF
                     MFSMSIPLSCWMEDSYVNYSLTQNNHHESTMQVGLNGTMLEGRNLSYNVQESWMHSPD
                     DSYSGNAGMTYDGTYGSVNGSYSWSRDSQHFDYGARGGVLVHSDGVTFSQELGETVAL
                     VKAPGAEGLSIENATGISTDWRGYTVKTQLSPYDENRVALNSDYFSKANIELENTVIN
                     LVPTRGAVVKAEFVTHVGYRVLFNVRQVNGKPIMFGAMATASLETGTVTGIVGDNGEL
                     YLSGMPEKGEFLLSWGQAADEKCKAAYHITHKPDDTSLVQMDAICR"
     gene            3287..4357
                     /locus_tag="N7C74_003581"
     CDS             3287..4357
                     /locus_tag="N7C74_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2433381.1"
                     /translation="MKKILSGLILLLCCPYGFAANGDGATHMSNLSFGPLTVAAANNH
                     SGYNIFEALSNTTGTYPVRCHCDDTHGGPGQQTAFFPIFYTGDAAPGLVLERTLNGLN
                     YYALNDYLSVGVTIFIINNQYAAIPFEHLSNQSTSPQHTCGAGNNGSTVNLDSGRSAK
                     LSFYVRHSITGTVTIPTTEVAWLYAGMSDHFPKTTPVSKVTIRGQLTAPQNCELTPNQ
                     SIDVDFQKINSAEFSSTAGSIIAERKIKTEVTVSCTGMEDVRSTEVVSASMIAANRSA
                     DATMIVTSNPDVGIKIFDKNDRPVNVDGGNLPADMGAISRLGKTDGSVTFYSAPASLT
                     GAKPAPDNGFTATATLVIEFTN"
     gene            4385..5467
                     /locus_tag="N7C74_003582"
     CDS             4385..5467
                     /locus_tag="N7C74_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2433382.1"
                     /translation="MKKWHYIFCIILFHLGLPCGYAANDGTCATRGGTHTLSLNFPLT
                     TVSAANNVPGNTLIDIANATSSENYSVLCNCDSKHSNGAYHEIYYTADPAPGMVYSTT
                     ASGLAFYYLNEYVDVGTKISVLNAGYTAVPFEHVSNQATTTDHTCQGNKTTAVGVSLK
                     TGADAKISFRIKRSINGTVVIPITDIALLYANISSTTTRGEAIAKVRISGSLTAPQSC
                     QINAGQVIYFDFDTIPASEFSSTAGQAITSRKITKTVSIECTGMGYERTQKVDASFTG
                     TNRSSDDTMVATDNADVGIKIYNKSNAEVSVNNGKLPADMGNTTIFGRKNGSVTFSAA
                     PASFTGARPQPGVFNATATLTIEFVN"
     gene            5679..6719
                     /gene="pstS"
                     /locus_tag="N7C74_003583"
     CDS             5679..6719
                     /gene="pstS"
                     /locus_tag="N7C74_003583"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HGU2433383.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVLPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            6806..7765
                     /gene="pstC"
                     /locus_tag="N7C74_003584"
     CDS             6806..7765
                     /gene="pstC"
                     /locus_tag="N7C74_003584"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HGU2433384.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            7765..8655
                     /gene="pstA"
                     /locus_tag="N7C74_003585"
     CDS             7765..8655
                     /gene="pstA"
                     /locus_tag="N7C74_003585"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HGU2433385.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            8838..9611
                     /gene="pstB"
                     /locus_tag="N7C74_003586"
     CDS             8838..9611
                     /gene="pstB"
                     /locus_tag="N7C74_003586"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGU2433386.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            9626..10351
                     /gene="phoU"
                     /locus_tag="N7C74_003587"
     CDS             9626..10351
                     /gene="phoU"
                     /locus_tag="N7C74_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HGU2433387.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDPKE"
BASE COUNT         2718 a         2509 c         2670 g         2717 t
ORIGIN      
        1 gcgtcattat tatatttttt atgagataca tttatgccta tgtatttact tagattgttt
       61 tttgctgtta ctttgattac gcagatgtcg atggctacgg ctggtattgt tgttggggga
      121 actcgcgtta ttttttatgg tgacaaacct gataccacaa tctctatttt taataaagaa
      181 acgaaccttc cttatctatt gcagacatgg gttgatccat ttaataaaga ggataaaagc
      241 aaaccgccat tttctgtgat acctcccgtt tctcgcattg aaccacagca agagaaaata
      301 ttacgcattt taaaaacggc tggcgagtta ccaaaagatc gggagtccgt attctggttg
      361 aacgtgaaaa acatccctcc ttcaattgat aaaaaaatac caatgttctt gagatagcca
      421 taaaaacaag aattaagtta ttctggcgac cagccgcggt aaagataatc cctgagcagg
      481 ctattaaaaa tgttaaatgg tcgcggcagg gtaataatct catcgtcgaa aatccaaatc
      541 cgatccatat taatgtaatg agtgtgacgg tggatgataa agacgtgtcg ctaaatatga
      601 tccgcccttt tgaaatattg acattaccaa ttccggctgg cgtggcagga aaatcgctcg
      661 tttggcgctt tattaatgat tacggtgcaa ttagtcaacc actaaagaaa aacctttaag
      721 cgccggataa ggatatatcg atggcctctt cgcatctttt cattacgctt gcatcgggca
      781 tatgtctgct ctgttccata tctgcttttg cccgggatag cttgttcaac cccagattac
      841 tggaactgga tcatcctgcg gataatattg atattcacca gttcaaccgt tcgaatacct
      901 tacctgcggg aacatacaaa gttgatgtga tgatcaacgg catgctcttc gaacgccagg
      961 aagttaaatt cgcccaggat aaccctgatg ctgaactcca cccatgctac gtggcgataa
     1021 aaaacgtgct ggcgacctat ggtataaaag ttgatgcgat aaaatctctg gcgaatgttg
     1081 atgacaaaac atgcgtaaat ccagttccgc tgatcgacgg ggctacctgg ttactggacg
     1141 ccagtaaact tgcattgaat attactattc cgcaaattta tctcaacaat gcagttaatg
     1201 gttatatcag cccttcccgt tgggatcagg ggatcaatgc catgatgatg aattatgatt
     1261 tttcggcatc gcataccatc cggtcaaatt atgacgacga cgatgacagt tattatctga
     1321 atttgcgtaa tggtattaat ttaggcgcat ggcgttttcg taattacagc accctgaatt
     1381 cttatgacgg taatgtggac taccattccg tcagtaatta cattcagcgc gacatcatgg
     1441 cattacgtag ccagattatg attggcgata cctggacggc aagcgatgta tttgatagta
     1501 cacaggtgcg tggcgtgcgg ctgtataccg atgacgatat gttgccctcc agccagaacg
     1561 gctttgcgcc agtggtacat gggattgcga aaactaacgc cacggtgatc atcaaacaaa
     1621 acggctacgt tatttatcaa tcagccgtac cacagggcgc atttgccctc accgacttaa
     1681 acacgaccag tagcggcggc gatctcgatg tcactatcaa agaagaagat ggcagcgagc
     1741 agcactttat tcagccattt acttcactgg ccattctcaa gcgtgaaggt cagaccgatg
     1801 tagaccttag cattggagaa gtgcgcgacg aaagcggctt tacgcctgag gtcttgcagt
     1861 tacaagcaat gcacggtttc cctttgggaa taactttgta tggcggaaca caattggcaa
     1921 atgattacgc ttctgccgcg ctgggtattg gtaaagatat gggggcgctg ggcgcgattt
     1981 cttttgacgt gactcatgcc cgctcgcagt ttgactacga cgataatgag agtggtcaat
     2041 cgtatcgttt tctctattcc aaacgttttg aagacaccaa taccaccttt cgtctggtgg
     2101 gttatcgcta ctctatggag gggttctaca ccctcaatga atgggtgtcg cgacaggata
     2161 atgattctga tttctgggta acgggcaacc gtcgcagccg cttcgaaggt acctggacgc
     2221 aatctttcac gccaggctgg ggcaatattt atttaacatt cagtcgacag gaatactggc
     2281 agaccgatga ggtcgaacgt ttattacagt tcggctataa caacaactgg cgaaacatct
     2341 cctggaacgt ttcctggaac tatacggact cgatcaagcg ctcattgggc aaccatcatg
     2401 atgataacaa tgatgatttc ggcaaagaac agattttcat gttctcaatg tcgataccgc
     2461 tatcgtgctg gatggaagac agctacgtca actattcgtt aacgcaaaac aaccaccatg
     2521 aaagcacgat gcaggtcggt ctgaacggaa cgatgctgga agggcgtaac ctgtcttata
     2581 acgtacagga atcgtggatg cactctcctg atgactccta cagcggcaat gccggaatga
     2641 cctatgacgg gacttatggc tcggtcaatg gtagctattc ctggagccgt gactcccaac
     2701 attttgatta tggcgccaga ggcggcgtgc tggtgcatag tgacggagtg accttctcgc
     2761 aggaactggg cgaaacggtg gcattggtca aagcgccggg cgcagaaggc ctgtccattg
     2821 aaaacgccac cgggatttct accgactggc gtggttatac cgtaaaaacg cagcttagcc
     2881 cgtatgacga aaaccgcgtg gcattgaaca gcgactattt ctccaaagcc aatattgaac
     2941 tggaaaacac cgtcatcaac ctggtaccaa cgcgcggtgc ggtggtgaaa gccgaatttg
     3001 tcacccatgt cggttatcgc gtgctattta acgtccgcca ggtcaacggt aaaccaataa
     3061 tgtttggcgc gatggcaacc gcctctctcg aaacgggcac agtcaccggg attgtcggtg
     3121 ataacggcga actgtatctc tccgggatgc ctgaaaaagg cgagttttta ttgagttggg
     3181 gacaagctgc ggatgaaaaa tgtaaggcgg cctatcacat cacccataaa cctgatgata
     3241 ccagcctggt tcaaatggat gcgatttgtc gctaaaggaa gcatgaatga aaaaaatatt
     3301 atcagggttg attctgctgc tttgctgtcc ttatggtttc gccgctaatg gtgatggcgc
     3361 aacgcacatg tcaaatttat catttggtcc gctgacggtg gcagcggcga ataaccactc
     3421 cggatacaat attttcgagg cactgagcaa cacgactgga acatacccgg tgcgctgtca
     3481 ctgtgatgac acgcatggcg gaccgggcca acaaacagca ttttttccta tcttctacac
     3541 gggagatgcc gcaccggggc ttgtgcttga gcgcactctt aatgggttaa attactatgc
     3601 tctgaatgat tatttatcgg tcggcgtgac gatttttatt attaataacc aatatgcggc
     3661 cattcctttt gaacacttat ccaaccaatc cacctcaccg caacatacct gtggagcagg
     3721 taataatggg agcactgtaa atctggattc agggcgctcg gcaaaattat ctttctatgt
     3781 tcggcattct attactggca cggtgacaat acccacaacg gaagtcgcct ggttgtacgc
     3841 gggcatgtcc gatcattttc ccaaaacgac ccccgtttct aaagtgacaa ttcgcggaca
     3901 actaacggcc ccgcagaact gtgagttaac gccaaatcag agcatcgatg tcgattttca
     3961 aaaaattaat agcgctgagt tctcctcaac ggcgggttca attattgcag aaagaaagat
     4021 taaaaccgaa gtcacagtat cttgtaccgg gatggaagac gtaaggtcca cggaggtggt
     4081 gagtgcgtcg atgattgcgg caaacagaag tgccgatgcc accatgatcg tgacgagtaa
     4141 tccggatgtg ggaattaaga tttttgataa gaacgaccgt ccagtgaatg tggatggggg
     4201 caacttaccc gctgatatgg gtgctattag tcgattagga aaaaccgatg gtagcgtaac
     4261 gttttattca gcgcccgcca gtctgacggg cgcaaaacca gcgcctgata atggatttac
     4321 cgctacagcc acgctggtta ttgaatttac taactaatct tgtgttagta aaaggaataa
     4381 tttcatgaaa aaatggcatt atatattttg cataattctc tttcatttag ggttaccgtg
     4441 cgggtatgcg gcaaatgatg gcacgtgtgc aacaagaggc ggcacacata cattaagcct
     4501 taattttcct ctgacaacgg tcagtgcagc aaacaatgtg cctggaaata cattaataga
     4561 tattgctaat gcaacatctt ctgaaaatta tagcgttctg tgtaactgtg attcaaaaca
     4621 tagcaatggc gcttatcacg aaatatatta taccgcagac cctgctcccg gtatggttta
     4681 tagcaccacc gcaagtggtc ttgcttttta ctatcttaac gaatatgtcg atgtgggaac
     4741 aaaaatatct gtgctaaatg cggggtatac ggcagttcct tttgaacatg tttccaacca
     4801 ggcaactaca acagatcaca cttgtcaggg aaacaaaact acagcggttg gcgtgagcct
     4861 gaaaactgga gcagatgcga agatttcatt tcgtattaaa cgttcaataa atggaacggt
     4921 agtaatacct atcaccgata ttgcattgct gtatgccaac atatccagca ccacgacccg
     4981 tggtgaggcg attgcaaaag ttcgaatttc aggcagtttg accgcaccac agtcttgtca
     5041 gataaatgca ggacaggtga tttattttga ttttgatact attcctgcgt ccgaattttc
     5101 atctaccgcc gggcaagcca ttacttcacg aaaaatcact aaaacagtga gtattgagtg
     5161 tacggggatg gggtatgagc gtacgcagaa agtcgatgct tcttttacgg ggacgaaccg
     5221 aagcagtgac gatacgatgg tggcgacaga caatgctgat gtcgggatca aaatttacaa
     5281 taaatcgaat gctgaagtta gcgtcaacaa cggcaagtta cccgcagaca tgggcaacac
     5341 gaccattttt ggtcgtaaaa atggttcggt aactttttcg gcagcacctg ccagctttac
     5401 cggtgcccgg cctcagcccg gcgtttttaa cgctaccgcg accttaacca ttgaatttgt
     5461 aaactaacct tttgcggctg ttttatgaca gccgttcata tttcctctcc tttcatgaaa
     5521 aaatgctgat atttttcccc ttcaatatca ataagtaata actttccttt gggtgtcatc
     5581 aaactgtcac attccttaca tataactgtc acctgtttgt cctattttgc ttctcgtagc
     5641 caacaaacaa tgctttatga atcctcccag gagacattat gaaagttatg cgtaccaccg
     5701 tcgcaactgt tgtcgccgcg accttatcga tgagtgcttt ctctgtgttt gcagaagcaa
     5761 gcctgacagg tgcaggtgca accttccctg cgccggtgta tgccaaatgg gctgacactt
     5821 accagaaaga aaccggtaat aaagttaatt accagggtat cggttcttcc ggtggcgtaa
     5881 aacagattat cgccaatacc gttgattttg gtgcttctga cgcgccgctg tctgacgaaa
     5941 aactggctca ggaaggtctg ttccagttcc cgaccgtgat tggcggcgtg gtgctggcgg
     6001 ttaacattcc aggtctgaag tctggtgagc tggtgctgga tggtaaaacc ctcggcgata
     6061 tctacctggg caaaatcaag aagtgggatg atgaagccat cgccaaattg aatccgggtc
     6121 tgaaactgcc ttcacagaac attgctgtag tgcgccgcgc agatggctcc gggacttcct
     6181 tcgtcttcac cagctacctg gcgaaagtga atgaagagtg gaaaaacaac gttggtactg
     6241 gctctaccgt aaaatggccg atcggtctgg gtggtaaagg taacgacggt atcgctgcgt
     6301 tcgttcagcg tctgccgggt gcaattggtt acgttgaata tgcttatgcg aagcaaaaca
     6361 acctggcgta caccaaactg atctccgctg atggtaaacc tgttttgccg accgaagaaa
     6421 acttcgctaa tgcagcaaaa ggtgcagact ggagcaaaac cttcgctcag gatctgacca
     6481 accagaaagg cgaagatgcg tggcctatta cctctaccac gttcattctg atccacaaag
     6541 atcagaagaa accagaacaa ggcacagaag tgctgaaatt cttcgactgg gcgtacaaaa
     6601 ccggggctaa acaggcgaac gacctggatt acgccagcct gccggatagc gtagttgaac
     6661 aggttcgcgc tgcgtggaag accaatatta aagacagtag cggcaagccg ctgtactaat
     6721 aaaactccag gccggataag gcattaaacg gcttatccgg cattacaaaa tgactttgta
     6781 aacgcgttta actgaagagt aacttatggc tgcaaccaag cctgctttta acccaccggg
     6841 taaaaagggc gacataattt tcagcgtgct ggtaaaactg gcggcgctga ttgtgctatt
     6901 gatgttgggt ggcattattg tctctctgat catctcctca tggccgagca ttcagaaatt
     6961 tggtctggct ttcctgtgga ccaaagagtg ggatgcgccg aacgatatct acggggcgct
     7021 ggtgccgatc tacggtacgt tggtgacttc gtttatcgcg ctgctgatcg ccgtcccggt
     7081 gagtttcggt atcgccctgt tcctgactga gcttgcgcct ggctggctga aacgcccgct
     7141 gggtatcgcc attgagctgc tggcagccat tccaagtatc gtttacggca tgtggggcct
     7201 gtttatcttt gcgccgctgt tcgccgttta cttccaggag ccggtcggca atatcatgtc
     7261 gaatatcccg attgtcggcg cgctgttctc tggcccggca tttggtatcg gtatcctcgc
     7321 ggcaggcgtg atcctcgcca tcatgattat tccgtacatt gcggcggtaa tgcgcgatgt
     7381 gttcgaacaa accccggtga tgatgaaaga gtcggcctac ggtattggct gcaccacctg
     7441 ggaggttatc tggcgtatcg ttcttccgtt caccaaaaat ggtgttatcg gcggcattat
     7501 gctggggctg ggccgcgcgc ttggtgaaac catggcggtg acctttatta tcggtaacac
     7561 ctaccagctc gacagcgcct cgctgtacat gccgggcaac agtatcacct ctgcgctggc
     7621 gaacgaattt gccgaagcgg aatccggtct gcacgttgcc gcactgatgg aactgggcct
     7681 gatcctgttt gtgattacct tcatcgtcct cgccgcatcg aagtttatga ttatgcgcct
     7741 ggctaagaat gagggggctc gctaatggct atggttgaaa tgcaaaccac tgcggcgctg
     7801 gcggaatccc gccgcaaaat gcaggcgcgt cgccgcctca aaaaccgtat tgcactgacg
     7861 ctctcaatgg cgacgatggc cttcggtctg ttctggctga tctggatttt aatgtccacc
     7921 atcacccgcg gtatcgacgg tatgtcgctg gcgctgttca ctgaaatgac gccgccgccc
     7981 aatacggaag gtggtggtct ggcgaacgct ctggcaggta gcgggctgtt aattttgtgg
     8041 gccacggtat tcggtacgcc gctgggcatt atggcgggga tttatctggc tgaatatggt
     8101 cgtaaatcct ggctggcaga agtgattcgc ttcattaacg acattctgct ctctgcgccg
     8161 tcgattgtgg ttggtctgtt tgtttacacc atcgtggtgg cgcagatgga gcacttctcc
     8221 ggttgggcgg gtgtgattgc gctggcgttg ttgcaggtgc cgattgttat ccgcaccacc
     8281 gagaacatgc tgaaactggt gccggacagc ctgcgtgaag cggcttatgc gctgggtaca
     8341 ccgaagtgga agatgatctc cgcgattacg ctgaaagcgt cggtgtccgg gatcatgacc
     8401 ggtatcctgc tggcgattgc ccgtattgct ggtgaaaccg cgccgctgct gtttaccgcg
     8461 ctctccaacc agttctggag cacggacatg atgcagccga tcgccaacct gccggtgacg
     8521 atctttaagt ttgcgatgag cccgtttgcg gaatggcagc aactggcctg ggccggggta
     8581 ttgatcatta ccctgtgcgt actgctgctg aacattctgg cgcgcgttgt tttcgcgaag
     8641 aataaacacg gttgatattg ctgacacggt tttcccctca ccctaaccct ctccccagag
     8701 gggcgagggg accgaccgag cgcctttttg actctgtaca cggttaacac tttgccggat
     8761 gcggcgtgaa cgcctgatcc ggcctacggt aagcctgatt cgcgaagtgc atcaggcacg
     8821 atgaggaaaa gattgcaatg agtatggttg aaactgcccc gagtaaaatt caggttcgta
     8881 atttgaactt ctactacggc aaattccatg ccctgaaaaa catcaacctg gatatcgcca
     8941 aaaaccaggt aacggcgttt atcgggccgt ccggctgcgg taaatcgacg ctgctgcgta
     9001 ccttcaacaa aatgtttgaa ctgtacccgg aacagcgtgc ggaaggtgaa attctgctcg
     9061 atggcgacaa catcctgacc aactctcagg atatcgccct gctgcgtgcg aaagtgggca
     9121 tggtgttcca gaaaccgacg ccgttcccga tgtccatcta cgacaacatc gcctttggcg
     9181 ttcgtctgtt tgagaagctc tctcgtgccg acatggacga gcgcgtgcag tgggcattga
     9241 ccaaagccgc attgtggaac gaaaccaaag ataaattgca tcagagcggt tactctctct
     9301 ctggtggtca gcaacagcgt ctgtgcattg cgcgtggtat cgccattcgc ccggaagtgc
     9361 tgctgctcga cgaaccatgt tcggcgctcg accccatctc caccgggcgt attgaagagc
     9421 tgatcaccga actgaagcag gattacaccg tggtgatcgt cacccacaac atgcagcagg
     9481 ctgcgcgttg ttccgaccac acggcgttta tgtacctggg cgaattgatt gagttcagca
     9541 acacggacga tctgttcacc aagccagcga agaaacaaac agaagactac atcaccggtc
     9601 gttacggttg attcaggagt gcgttatgga cagtctcaat cttaataaac atatttccgg
     9661 ccagttcaac gccgaactgg aaagtatccg cacgcaggtg atgaccatgg gcggcatggt
     9721 ggagcagcag ctttctgatg caatcaccgc gatgcataac caggacagcg atctggcgaa
     9781 gcgcgtgatc gaaggcgaca agaacgtcaa catgatggaa gtggcgatcg atgaagcctg
     9841 cgtgcgcatt attgccaaac gccagccgac ggcgagcgac ctgcgattgg ttatggtgat
     9901 cagtaaaacc attgccgaac tggagcgtat tggcgacgtg gcggacaaaa tctgccgtac
     9961 tgcgctggag aaattctccc agcagcatca gccgttgctg gtaagtttgg agtcgctggg
    10021 ccgtcatact atccagatgc tgcacgacgt gctggacgcg ttcgcgcgga tggacattga
    10081 cgaagcggta cgtatttatc gtgaagataa aaaagtcgat caggagtacg aaggtattgt
    10141 tcgtcaactg atgacctaca tgatggaaga ttcgcgcacc attccgagtg tacttaccgc
    10201 gctgttctgt gcgcgttcta tcgaacgtat tggcgaccgc tgccagaata tttgtgagtt
    10261 tatcttctac tacgtgaagg gtcaggattt ccgtcatgta ggcggtgatg agctggataa
    10321 actgctggcg ggaaaagacc ccaaagagta gtgctaactg taggccagat aaagcttttt
    10381 gccgcatctg gcaatcagcc cggtcataca tagttaccgg gctgatacac tttctcactg
    10441 aaatattgcc acacaaactc acctcgaaac tctccccagt acattgaata tacattattt
    10501 catttgaatt attttacttt taatgtagtg cctcgctata aaaacatatt tgttattctt
    10561 ttcatttata tcgctacctg cacagagaat ggaaaagaaa acaaaaaaca gggt
//
LOCUS       DBFIFM010000160        10508 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.160, whole genome shotgun sequence.
ACCESSION   DBFIFM010000160 DBFIFM010000000
VERSION     DBFIFM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.160"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            304..708
                     /gene="sdhC"
                     /locus_tag="N7C74_003588"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             304..708
                     /gene="sdhC"
                     /locus_tag="N7C74_003588"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HGU2433388.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            702..1049
                     /gene="sdhD"
                     /locus_tag="N7C74_003589"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             702..1049
                     /gene="sdhD"
                     /locus_tag="N7C74_003589"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HGU2433389.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFCASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            1049..2815
                     /gene="sdhA"
                     /locus_tag="N7C74_003590"
     CDS             1049..2815
                     /gene="sdhA"
                     /locus_tag="N7C74_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HGU2433390.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2831..3547
                     /gene="sdhB"
                     /locus_tag="N7C74_003591"
     CDS             2831..3547
                     /gene="sdhB"
                     /locus_tag="N7C74_003591"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HGU2433391.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3848..6649
                     /gene="sucA"
                     /locus_tag="N7C74_003592"
     CDS             3848..6649
                     /gene="sucA"
                     /locus_tag="N7C74_003592"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HGU2433392.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6664..7881
                     /gene="odhB"
                     /locus_tag="N7C74_003593"
     CDS             6664..7881
                     /gene="odhB"
                     /locus_tag="N7C74_003593"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HGU2433393.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            7975..9141
                     /gene="sucC"
                     /locus_tag="N7C74_003594"
     CDS             7975..9141
                     /gene="sucC"
                     /locus_tag="N7C74_003594"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HGU2433394.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            9141..10010
                     /gene="sucD"
                     /locus_tag="N7C74_003595"
     CDS             9141..10010
                     /gene="sucD"
                     /locus_tag="N7C74_003595"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HGU2433395.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
BASE COUNT         2491 a         2717 c         2929 g         2371 t
ORIGIN      
        1 gttgttatcg tgacctggat cactgttcag gataaaaccc gacaaactat atgtaggtta
       61 attgtaatga ttttgtgaac ggcctatact gccgccaggt ctccggaaca ccctgcaatc
      121 ccgagccacc cagcgttgta acgtgtcgtt ttcgcatctg gaagcagtgt tttgcatgac
      181 gcgcagttat agaaaggacg ctgtctgacc cgcaagcaga ccggaggaag gaaatcccga
      241 cgtctccagg taacagaaag ttaacctctg tgcccgtagt ccccagggaa taataagaac
      301 agcatgtggg cgttattcat gataagaaat gtgaaaaaac aaagacctgt taatctggac
      361 ctacagacca tccggttccc catcacggcg atagcgtcca ttctccatcg cgtttccggt
      421 gtgatcacct ttgttgcagt gggtatcctg ctgtggcttc tgggtaccag cctctcttcc
      481 cctgaaggtt tcgagcaagc ttccgcgatt atgggcagct tcttcgtcaa atttatcatg
      541 tggggcatcc ttaccgctct ggcgtatcac gtcgtcgtag gtattcgcca catgatgatg
      601 gattttggct atctggaaga aacattcgaa gcgggtaaac gctccgccaa aatctccttt
      661 gttattactg tcgtgctttc acttctcgca ggagtcctcg tatggtaagc aacgcctccg
      721 cattaggacg caatggcgta catgatttca tcctcgttcg cgctaccgct atcgtcctga
      781 cgctctacat catttatatg gtcggttttt tcgccaccag tggcgagctg acatatgaag
      841 tctggatcgg tttctgcgcc tctgcgttca ccaaagtgtt caccctgctg gcgctgtttt
      901 ctatcttgat ccatgcctgg atcggcatgt ggcaggtgtt gaccgactac gttaaaccgc
      961 tggctttgcg cctgatgctg caactggtga ttgtcgttgc actggtggtt tacgtgattt
     1021 atggattcgt tgtggtgtgg ggtgtgtgat gaaattgcca gtcagagaat ttgatgcagt
     1081 tgtgattggt gccggcggcg caggtatgcg cgcggcgctg caaatttccc agagcggcca
     1141 gacctgtgcg ctgctctcta aagtcttccc gacccgttcc cataccgttt ctgcgcaagg
     1201 cggcattacc gttgcgctgg gtaataccca tgaagataac tgggaatggc atatgtacga
     1261 caccgtgaaa gggtcggact atatcggtga ccaggacgcg attgaatata tgtgtaaaac
     1321 cgggccggaa gcgattctgg aactcgaaca catgggcctg ccgttctcgc gtctcgatga
     1381 tggtcgtatc tatcaacgtc cgtttggcgg tcagtcgaaa aacttcggcg gcgagcaggc
     1441 ggcacgcact gcggcagcgg ctgaccgtac cggtcacgca ctgttgcaca cgctttatca
     1501 gcagaacctg aaaaaccaca ccaccatttt ctccgagtgg tatgcgctgg atctggtgaa
     1561 aaaccaggat ggcgcggtgg tgggttgtac cgcactgtgc atcgaaaccg gtgaagtggt
     1621 ttattttaaa gcccgcgcta ccgtgctggc gactggcgga gcagggcgta tttatcagtc
     1681 taccactaac gcccacatta acaccggcga cggcgtcggc atggctatcc gcgcaggcgt
     1741 accggtgcag gatatggaaa tgtggcaatt ccacccgacc ggcattgccg gtgcgggcgt
     1801 actggtcacc gaaggttgcc gtggtgaagg cggttatctg ctgaacaaac atggcgaacg
     1861 ctttatggag cgttatgcgc cgaacgccaa agacctggcg ggccgtgatg tggtggcgcg
     1921 ttcaatcatg attgaaatcc gtgaaggtcg cggctgtgat ggtccgtggg ggccacacgc
     1981 gaaactgaaa ctcgatcacc tgggtaaaga agttctcgaa tcccgtctgc cgggtatcct
     2041 ggagctttcc cgtaccttcg ctcacgttga tccggtgaaa gagccgattc cggttatccc
     2101 aacctgtcac tacatgatgg gcggtattcc gaccaaagtt accggtcagg cgctgactgt
     2161 gaatgagaaa ggcgaagatg tggttgttcc ggggctgttc gcggtaggtg aaattgcctg
     2221 cgtatcggta cacggtgcta accgtctggg cggcaactcg ctgctggacc tggtggtctt
     2281 tggtcgcgca gcgggtctgc atctgcaaga gtctatcgcc gagcagggcg cattgcgcga
     2341 tgccagcgag tctgatgttg aagcatcttt ggatcgtctg aaccgctgga acaataatcg
     2401 taacggtgaa gatccggtgg cgattcgtaa agcgctgcaa gaatgtatgc agcataactt
     2461 ctcggtcttc cgtgaaggtg atgcgatggc taaagggctt gagcagttga aagtgatccg
     2521 cgagcgtctg aaaaatgccc gtctggatga cacttccagc gagttcaaca cccagcgcgt
     2581 tgagtgcctg gaactggata acctgatgga aacggcgtat gcaacggctg tttctgccaa
     2641 cttccgtacc gaaagccgtg gcgcacatag ccgcttcgac ttcccggatc gtgatgatga
     2701 aaactggctg tgccactccc tgtatctgcc agagtcggaa tccatgacgc gccgaagcgt
     2761 caacatggaa ccgaaactgc gcccggcatt cccgccgaag attcgtactt actaatgcgg
     2821 agacaggaaa atgagactcg agttttcaat ttatcgctat aacccggatg ttgatgatgc
     2881 tccgcgtatg caggattaca ccctggaagc ggaagaaggt cgcgacatga tgctgctgga
     2941 tgcgcttatc cagctgaaag agaaagatcc cagcctgtcg ttccgccgct cctgccgtga
     3001 aggtgtgtgc ggttccgacg gtctgaacat gaacggcaag aatggtctgg cctgtattac
     3061 cccgatttcg gcactcaacc agccgggcaa gaagattgtg attcgcccgc tgccaggttt
     3121 accggtgatc cgcgatttgg tggtagacat gggacaattc tatgcgcaat atgagaaaat
     3181 taagccttac ctgttgaata atggacaaaa tccgccagct cgcgagcatt tacagatgcc
     3241 agagcagcgc gaaaaactcg acgggctgta tgaatgtatt ctctgcgcat gttgttcaac
     3301 ctcttgtccg tctttctggt ggaatcccga taagtttatc ggcccggcag gcttgttagc
     3361 ggcatatcgt ttcctgatcg atagccgtga taccgagact gacagccgcc tcgacggttt
     3421 gagcgatgca ttcagtgtat tccgctgtca tagcatcatg aactgcgtca gtgtatgtcc
     3481 gaaggggctg aacccgacgc gcgccatcgg ccatatcaag tcgatgttgt tgcaacgtaa
     3541 tgcgtaaacc gtaggcctga taagacgcgc aagcgtcgca tcaggcaacc agtgccggat
     3601 gcggcgtgaa cgccttatcc ggcctacaag tcattacccg taggcctgat aagcgcagcg
     3661 catcaggcgt aacaaagaaa tgcaggaaat ctttaaaaac tgcccctgac actaagacag
     3721 tttttaaagg ttccttcgcg agccactacg tagacaagag ctcgcaagtg aaccccggca
     3781 cgcacatcac tgtgcgtggt agtatccacg gcgaagtaag cataaaaaag atgcttaagg
     3841 gatcacgatg cagaacagcg ctttgaaagc ctggttggac tcttcttacc tctctggcgc
     3901 aaaccagagc tggatagaac agctctatga agacttctta accgatcctg actcggttga
     3961 cgctaactgg cgttcgacgt tccagcagtt acctggtacg ggagtcaaac cggatcaatt
     4021 ccactctcaa acgcgtgaat atttccgccg cctggcgaaa gacgcttcac gttactcttc
     4081 aacgatctcc gaccctgaca ccaatgtgaa gcaggtaaaa gtcctgcagc tcattaacgc
     4141 ataccgcttc cgtggtcacc agcatgcgaa tctcgatccg ctgggactgt ggcagcaaga
     4201 taaagtggcc gatctggatc cgtctttcca cgatctgacc gaagcagact tccaggagac
     4261 cttcaacgtc ggttcatttg ccagcggcaa agaaaccatg aaactcggcg agctgctgga
     4321 agccctcaag caaacctact gcggcccgat tggtgccgag tatatgcaca ttaccagcac
     4381 cgaagaaaaa cgctggatcc aacagcgtat cgagtctggt cgcgcgactt tcaatagcga
     4441 agagaaaaaa cgcttcctaa gcgaactgac cgccgctgaa ggccttgaac gttacctcgg
     4501 tgcaaaattc cctggcgcaa aacgcttctc gctggaaggc ggtgacgcgt taatcccgat
     4561 gcttaaagag atgattcgcc acgctggcaa cagcggcacc cgcgaagtgg ttctcgggat
     4621 ggcgcaccgt ggtcgtctga acgtgctggt gaacgtgctg ggtaaaaaac cgcaagactt
     4681 gttcgacgag tttgccggta aacataaaga acacctcggc acgggtgacg tgaaatacca
     4741 catgggcttc tcgtctgact tccagaccga tggcggcctg gtacatctgg cgctggcgtt
     4801 taacccgtct caccttgaga ttgtcagccc ggtcgttatc ggttctgttc gtgcccgtct
     4861 ggacagactt gatgagccga gcagcaacaa agtgctgcca atcaccattc acggtgacgc
     4921 cgcagtgacc gggcagggcg tggttcagga aactctgaac atgtcgaaag cgcgtggtta
     4981 tgaagttggc ggtacggtac gtatcgttat caacaaccag gttggcttca ccacctctaa
     5041 cccgctggat gcccgttcaa cgccgtactg tactgatatc ggtaagatgg ttcaggcacc
     5101 gatttttcac gttaacgcgg atgatccgga agccgttgcc tttgtgaccc gtctggcgct
     5161 cgatttccgt aacaccttta aacgtgatgt cttcatcgac ctggtgtgct accgccgtca
     5221 cggccacaac gaagccgacg agccgagcgc aacccagccg ctgatgtatc agaaaatcaa
     5281 aaaacatccg acgccgcgca aaatctatgc tgacaagctg gagcaggaaa aagtggcgac
     5341 gctggaagat gccaccgaga tggttaacct gtaccgcgat gcgctggatg ctggcgattg
     5401 cgtagtggca gagtggcgtc cgatgaacat gcactctttc acctggtcgc cgtacctcaa
     5461 ccacgaatgg gacgaagagt acccgaataa agttgagatg aagcgcctgc aggagctggc
     5521 gaaacgcatc agcacggtgc cggaagcggt tgaaatgcag tctcgcgttg ccaaaattta
     5581 tggcgatcgc caggcgatgg ctgccggtga gaaactgttc gactggggcg gcgcggaaaa
     5641 cctcgcttac gccacgctgg ttgacgaagg cattccggtt cgcctgtcgg gtgaagactc
     5701 cggtcgcggt accttcttcc accgccacgc ggtgatccac aaccagtcta acggttccac
     5761 ttacacgccg ctgcaacaca tccataacgg ccagggcgcg ttccgtgtct gggactccgt
     5821 actttctgaa gaagcagtgc tggcgtttga atacggttat gccaccgcag aaccacgcac
     5881 tctgactatc tgggaagctc agttcggtga cttcgccaac ggtgcgcagg tggttatcga
     5941 ccagttcatc tcctctggcg aacagaaatg gggccggatg tgtggtctgg tgatgttgct
     6001 gccgcacggt tacgaagggc aggggccgga gcactcctcc gcgcgtctgg aacgttatct
     6061 gcaactttgt gctgagcaaa acatgcaggt gtgcgtaccg tctaccccgg cacaggttta
     6121 ccacatgctg cgtcgtcagg cgctgcgcgg gatgcgtcgt ccgctggtcg tgatgtcgcc
     6181 gaaatccctg ctgcgtcatc cgctggcggt ttccagcctc gaagaactgg cgaacggcac
     6241 cttcctgcca gccatcggtg aaatcgacga gcttgatccg aagggcgtga agcgcgtagt
     6301 gatgtgttct ggtaaggttt attacgacct gctggaacag cgtcgtaaga acaatcaaca
     6361 cgatgtcgcc attgtgcgta tcgagcaact ctacccgttc ccgcataaag cgatgcagga
     6421 agtgttgcag cagtttgctc acgtcaagga ttttgtctgg tgccaggaag agccgctcaa
     6481 tcagggcgca tggtactgca gccagcatca tttccgtgaa gtgattccgt ttggggcttc
     6541 tctgcgttat gcaggccgcc cggcctccgc ctctccggcg gtagggtata tgtccgttca
     6601 ccagaaacag caacaagatc tggttaatga cgcgctgaac gtcgaataaa taaaggatac
     6661 acaatgagta gcgtagatat tctggtccct gacctgcctg aatccgtagc cgatgccacc
     6721 gtcgcaacct ggcataaaaa acccggtgac gcagtcgtac gtgatgaagt gctggtagaa
     6781 atcgaaactg acaaagtggt actggaagta ccggcatcag cagacggcat tctggatgcg
     6841 gttctggaag atgaaggtac aacggtaacg tctcgtcaga tccttggtcg cctgcgtgaa
     6901 ggcaacagcg ccggtaaaga aaccagcgcc aaatctgaag agaaagcgtc cactccggcg
     6961 caacgccagc aggcgtctct ggaagagcaa aacaacgatg cgttaagccc ggcgatccgt
     7021 cgcctgctgg ctgaacataa cctcgacgcc agcgccatta aaggcaccgg tgtgggtggt
     7081 cgtctgaccc gtgaagatgt ggaaaaacat ctggcgaaag ctccggcgaa agagtctgca
     7141 ccggcagcgg ctgctccggc ggcgcaaccg gctctggctg cacgtagcga aaaacgtgtc
     7201 ccgatgactc gcctgcgtaa gcgtgtggca gagcgtctgc tggaagcgaa aaactccacc
     7261 gccatgctga ccacgttcaa cgaagtcaac atgaagccga ttatggatct gcgtaagcag
     7321 tacggtgaag cgtttgaaaa acgccacggc atccgtctgg gctttatgtc cttctacgtg
     7381 aaagcggtgg ttgaagccct gaaacgttac ccggaagtga atgcttctat cgacggcgat
     7441 gacgtggttt accacaacta tttcgacgtc agcatggcgg tttctacgcc gcgcggcctg
     7501 gtgacgccgg tactgcgtga tgtcgatacc ctcggcatgg cagacatcga gaagaaaatc
     7561 aaagagctgg cagttaaagg ccgtgacggc aagctgacgg ttgaagatct gaccggtggt
     7621 aacttcacca tcaccaacgg tggtgtgttc ggttccctga tgtctacgcc gatcatcaac
     7681 ccgccgcaga gcgcaattct gggtatgcac gctatcaaag atcgtccgat ggctgtgaat
     7741 ggtcaggttg agatcctgcc gatgatgtac ctggcgctgt cctacgatca ccgtctgatc
     7801 gatggtcgcg aatccgtggg cttcctggta acgatcaaag agttgctgga agatccgacg
     7861 cgtctgctgc tggacgtgta gtagtttaag tttcacctgc actgtagacc gacgtggata
     7921 gggtgtcggt ctacggttta aaagataacg attactgaag gatggacaga acacatgaac
     7981 ttacatgaat atcaggcaaa acaactgttt gcccgctatg gcttaccagc accggtgggt
     8041 tatgcctgta ctactccgcg cgaagcagaa gaagccgctt caaaaatcgg tgccggtccg
     8101 tgggtagtga aatgtcaggt tcacgctggt ggccgcggta aagcgggcgg tgtgaaagtt
     8161 gtaaacagca aagaagacat ccgtgctttt gcagaaaact ggctgggcaa gcgtctggta
     8221 acgtatcaaa cagatgccaa tggccaaccg gttaaccaga ttctggttga agcagcgacc
     8281 gatatcgcta aagagctgta tctcggtgcc gttgtagacc gtagttcacg tcgcgtggtc
     8341 tttatggcct ccaccgaagg cggcgtggaa atcgaaaaag tggcggaaga aactccgcac
     8401 ctgatccaca aagttgcgct tgatccgctg actggcccga tgccgtatca gggacgcgag
     8461 ctggcgttca aactgggtct ggaaggtaaa ctggttcagc agttcaccaa aatcttcatg
     8521 ggcctggcga ccattttcct ggagcgcgac ctggcgctga tcgaaatcaa cccgctggtc
     8581 atcaccaaac agggcgatct gatttgcctc gacggcaaac tgggcgctga tggcaacgcc
     8641 ctgttccgcc agcctgatct gcgcgaaatg cgtgaccagt cgcaggaaga tccgcgtgaa
     8701 gcacaggctg cacagtggga actgaactac gttgcgctgg acggtaacat cggttgtatg
     8761 gttaacggcg caggcctggc gatgggtacg atggacatcg ttaaactgca cggcggcgaa
     8821 ccggctaact tcctggacgt tggcggcggc gcaaccaaag aacgtgtaac cgaagcgttc
     8881 aaaatcatcc tctctgacga caaagtgaaa gccgttctgg ttaacatctt cggcggtatc
     8941 gttcgttgcg acctgatcgc tgacggtatc atcggtgcgg tagcggaagt gggtgttaac
     9001 gtaccggtcg tggtacgtct ggaaggtaac aacgccgaac tcggcgcgaa gaaactggct
     9061 gacagcggcc tgaatattat tgcagcaaaa ggtctgacgg atgcagctca gcaggttgtt
     9121 gccgcagtgg aggggaaata atgtccattt taattgataa aaacaccaag gttatctgcc
     9181 agggctttac cggtagccag gggactttcc actcagaaca ggccattgca tacggcacta
     9241 aaatggttgg cggcgtaacc ccaggtaaag gcggtactac ccacctcggc ctgccggtgt
     9301 tcaacaccgt gcgtgaagct gttgctgcta ctggcgctac cgcttctgtt atctacgtac
     9361 cagcaccgtt ctgcaaagac tccattctgg aagccatcga cgcaggcatc aaactgatta
     9421 tcaccatcac tgaaggcatc ccgacgctgg atatgctgac cgtgaaagtg aagctggatg
     9481 aagcaggcgt tcgtatgatc ggcccgaact gcccaggcgt tatcactccg ggtgaatgca
     9541 aaatcggtat tcagcctggt cacattcaca aaccgggtaa agtgggtatc gtttcccgtt
     9601 ccggtacact gacctatgaa gcggttaaac agaccacgga ttacggtttc ggtcagtcga
     9661 cctgtgtcgg tatcggcggt gacccgatcc ctggctctaa ctttatcgac attctcgaaa
     9721 tgttcgaaaa agatccgcag accgaagcga tcgtgatgat cggtgagatc ggcggtagcg
     9781 ctgaagaaga agcagctgcg tacatcaaag agcacgttac caagccagtt gtgggttaca
     9841 tcgctggtgt gactgcgccg aaaggcaaac gtatgggcca cgcgggtgcc atcattgccg
     9901 gtgggaaagg gactgcggat gagaaattcg ctgctctgga agccgcaggc gtgaaaaccg
     9961 ttcgcagcct ggcggatatc ggtgaagcac tgaaaactgt tctgaaataa atatctgtaa
    10021 taagaaatag ccctcgccgc ttccctctac aggaatggcg aagggctgtc ggtttcgaca
    10081 tggttggcca tcgtatgatg gcctttttta tgccttaaac ggttaaccac ggtcgaaatc
    10141 tgactgcagg cttgactgca tgaccacagc cactgtggat tgccgcaacc gaccgcaagc
    10201 cacgaataag aacgacggcg aatttcgaat cgttacctga tgcactggct tcccagcgtt
    10261 tgaccgtagc gttaagtaaa tcaacaaaag cgatgttcac cgcttttcgt caagtcgtgc
    10321 catttgtcag ccaggatcgg gtctttaccc cggatattgc agcatcgcaa aaattcttag
    10381 agggatatgc tataaatcaa aaataaaatc tttgttgatt gtttatcata atatccacag
    10441 atatgataaa tgatatcaat ttaataattt tcacttaatg tgattccgga taattcgaaa
    10501 ggcgctgt
//
LOCUS       DBFIFM010000161        10325 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.161, whole genome shotgun sequence.
ACCESSION   DBFIFM010000161 DBFIFM010000000
VERSION     DBFIFM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.161"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            147..1196
                     /gene="allD"
                     /locus_tag="N7C74_003596"
     CDS             147..1196
                     /gene="allD"
                     /locus_tag="N7C74_003596"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HGU2433396.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHVVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRQAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            1218..2453
                     /gene="allC"
                     /locus_tag="N7C74_003597"
     CDS             1218..2453
                     /gene="allC"
                     /locus_tag="N7C74_003597"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HGU2433397.1"
                     /translation="MITHFRQTIEEALPWLSSFGADPAGGMTRLLYSPEWLETQQQLK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVILSGSHIDTVVNGGNLDGQFGALAARL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPDAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIRIDIDLWMDEEPVPMNKDL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            2464..3249
                     /gene="allE"
                     /locus_tag="N7C74_003598"
     CDS             2464..3249
                     /gene="allE"
                     /locus_tag="N7C74_003598"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HGU2433398.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFS
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGYAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(3477..4622)
                     /gene="glxK"
                     /locus_tag="N7C74_003599"
     CDS             complement(3477..4622)
                     /gene="glxK"
                     /locus_tag="N7C74_003599"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HGU2433399.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMEECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(4644..5945)
                     /locus_tag="N7C74_003600"
     CDS             complement(4644..5945)
                     /locus_tag="N7C74_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HGU2433400.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTEVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(6002..7363)
                     /gene="allB"
                     /locus_tag="N7C74_003601"
                     /pseudo
     CDS             complement(6002..7363)
                     /gene="allB"
                     /locus_tag="N7C74_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            complement(7400..8791)
                     /locus_tag="N7C74_003602"
     CDS             complement(7400..8791)
                     /locus_tag="N7C74_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HGU2433401.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPLMEPLS
                     RVSWFVGVIVAFA"
     gene            complement(8960..9838)
                     /gene="glxR"
                     /locus_tag="N7C74_003603"
     CDS             complement(8960..9838)
                     /gene="glxR"
                     /locus_tag="N7C74_003603"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HGU2433402.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     NVDTARQVTEAADIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(9939..>10325)
                     /locus_tag="N7C74_003604"
     CDS             complement(9939..>10325)
                     /locus_tag="N7C74_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HGU2433403.1"
                     /translation="NMLMKEDILLLIEPINHFDIPGFHLTGTRQALKLIDDVGCCNLK
                     IQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGEINYDYLFKVIENSD
                     YNGWVGCEYKPQTTTEAGLRWMDPYR"
BASE COUNT         2761 a         2707 c         2439 g         2418 t
ORIGIN      
        1 ttgttttaaa aaccaggtta aaagcgagcg gattatcaaa taaattctaa ctatattttt
       61 ttgcctgtct ggatcacata atccagatat tttccctgta tgttaattgc agtcatgctt
      121 cacaccgtcg ttaaaaagga agacagatga aaatcagtcg ggaaacactc caccagctaa
      181 ttgagaataa actctgccag gctgggttaa aacgtgagca cgctgcaacc gtggctgaag
      241 tattggttta tgccgatgcc agagggatcc actctcatgg cgcggtgcgc gtggaatact
      301 acgcggaacg catttcaaaa ggcggcacca accgcgaacc ggagtttcgt cttgaggaaa
      361 ccgggccgtg ctcggcaatt ttacatgccg acaatgccgc cggacaggtc gcggcgaaaa
      421 tgggtatgga acatgccatc aaaaccgccc agcaaaatgg cgttgcggtg gtcggtatca
      481 gccggatggg tcacagcggc gcaatctctt attttgtgca gcaggcagcc cgcgccggat
      541 taattggcat ttcgatgtgc cagtccgatc cgatggtggt gccgtttggc ggcgcggaaa
      601 tttactacgg tactaaccca ctggcctttg ccgcgccggg agaaggcgac gagatcctta
      661 cctttgatat ggcgactacc gtacaggcat ggggaaaagt cctcgacgcc cgctcgcgta
      721 atatgtctat cccggatacc tgggcggtcg ataaaaacgg tgcaccaaca accgatccgt
      781 tcgcggtaca tgctctgctc cccgcagccg ggccgaaagg gtatggcctg atgatgatga
      841 ttgacgtcct ctcaggcgtc ttactcggct taccgttcgg gcgacaggtt agttcgatgt
      901 atgacgattt acacgccggg cgtaatttgg ggcaattaca tgtagttatt aacccgaact
      961 ttttctcctc cagcgaatta ttccgtcaac atcttagcca gaccatgcgc gaattaaatg
     1021 ccattacccc cgcgcccggt tttaatcagg tttattatcc cggacaggat caggatatta
     1081 aacaacgcca agccgccgtc gaaggcatcg aaattgttga tgatatttac cagtatttaa
     1141 tttccgacgc gctttataac acgtcatacg aaacgaaaaa tccctttgcg caataattat
     1201 tgagacagga ctttcttatg attacacatt ttcgtcaaac catagaagaa gcgctgccct
     1261 ggctttcctc ttttggcgct gacccagcgg gtgggatgac ccgtttactt tattcgccgg
     1321 aatggctgga aacccagcag caattaaaaa aaagaatggc cgcaagtggg ctggaaacac
     1381 gtttcgatga agtggggaat ttatacggtc gcctgagtgg caccgaatat ccacaggaag
     1441 tgattctgag cggttcgcat atcgataccg tggttaacgg cggtaacctc gacgggcaat
     1501 tcggcgcgct ggcggcgcgg ctggcaattg actggctgaa aactcaatac ggcgcaccgc
     1561 tgcgtacggt tgaagtggtg gcgatggcgg aagaagaagg tagccgcttc ccgtatgttt
     1621 tctggggcag taaaaatatc tttgggctgg cgaatcctga cgacgtgcgg aatatctgtg
     1681 atgccaaagg aaatagtttt gtcgatgcga tgaaggcttg cggatttact ctgccggacg
     1741 ccccgctaac tccgcgtcag gatattaaag cctttgtcga actgcatatc gaacagggct
     1801 gtgtgctgga aagtaatggg caatcaattg gcgtggtgaa tgcaattgtc gggcaacgtc
     1861 gctatacggt gacgctgaac ggcgaatcaa accatgcagg caccacgccg atgggttatc
     1921 gtcgtgatac ggtttacgct ttcagtcgca tttgccatca gtcgatcgaa aaagcgaaaa
     1981 agatgggcga tccgctggtt ctgacctttg ggaaagtaga gccgcgcccg aatacggtga
     2041 atgtggtgcc gggtaaaacc acgttcacca ttgattgtcg tcataccgac gccgccgtgc
     2101 tgcgtgattt cacccaacag ttagaaaacg acatgcgggc gatttgcgat gaaatggaca
     2161 ttcgtattga tatcgattta tggatggacg aagaacccgt gccgatgaat aaggacctgg
     2221 tcgccaccct gacagaattg tgtgaaagtg aaaaactgaa ttaccgggtg atgcacagtg
     2281 gtgccgggca cgacgcgcaa attttcgcgc ctcgcgtacc aacctgcatg atttttatcc
     2341 ccagcatcaa cgggatcagc cataacccgg cggaacgcac caatattacc gaccttgccg
     2401 aaggggtcaa aacgttggca ctcatgcttt atcaacttgc ctggcagaaa taaggagtca
     2461 taaatgggat atttaaataa cgtcaccggt taccgcgatg atttactggc taaccgtgcg
     2521 attgtgaaac acggtaattt cgcactgtta acaccagacg gtctggtaaa aaatattatt
     2581 ccgggctttg aaaattgtga cgcgacaatc ctctccacgc caaagctggg tgcctctttt
     2641 gttgattatc tggtcacact gcatcaaaac ggcggcaacc aacagggctt cggtggcgaa
     2701 ggcattgaaa cgttcctgta tgtgatctct ggaaatatca ctgccaaagc cgaaggcaaa
     2761 acattttcct taagcgaagg tggctatctt tattgcccgc ctggctcctt aatgacgttt
     2821 gttaacgccc aggccgaaga cagccaaatc tttttatata agcgccgcta tgttccggta
     2881 gaaggctatg caccgtggct ggtttctggc aatgccagcg aactggaacg cattcactat
     2941 gaaggtatgg atgatgtgat cctgctggat ttcctgccca aagagttagg ttttgatatg
     3001 aacatgcata tcctttcttt tgcaccaggt gccagccacg gttatatcga aacacatgtt
     3061 caggaacacg gtgcctatat tctttccggt cagggggttt ataacctcga caataactgg
     3121 atcccggtga aaaaaggcga ttacatcttt atgggcgctt attctttaca ggctggttat
     3181 ggcgtagggc gtggtgaagc gttcagctat atttactcga aagattgtaa ccgcgacgta
     3241 gagatttaag tcataccact cgcctgatta tttttagcta tgttggattt tgccacggtt
     3301 tataccgtgg ctttttttgc aacatagcct gatgcgcttc gcttatcagg cctacatgat
     3361 ctctgcaata tatagaattt gcgcgatttt caggaaggat atggtactca tgccgcatcc
     3421 ggcatgagta ctgcactctt atgcgatata aatcgcatcc gctttaaagg taagggtcag
     3481 tttttaattc cctgacctat tttaatggcg caggcaatat ttcgcgcgct gttgaagaga
     3541 ttggtttcac cgctggcgag cacttcggct aaaggtgcca gacgtggcaa aatgctgaat
     3601 accgcatcaa tgccgtactg gtgcaccact tccacgccat cacccaatac gccagcaatc
     3661 ccaatcaccg gtacattaaa ctgcttcgcc accgacgcaa cgcccagcgg cgctttaccg
     3721 cctgccgttt gcgagtcaat gcgcccttcc ccggtgatca ccagtgctgc cccctgcacc
     3781 gcctgctcca ggttgaccgc ccgcagtaca atctcaatac ccggtttaat atccgcattg
     3841 aggaataccg ccgccgcaat ccccataccg cccgcagcgc caccgcccgc catctggcag
     3901 acattaattt cagtttgctg ttgtaaaaca cgggcgtaat tttgcagccc ctgttcaagt
     3961 tcttcgacca tctccggcgt tgcccctttt tgcgggccaa acaccgccgc cgcgccgcgt
     4021 gcccctacca gcgggttatc aacgtcacat gctacttcaa tatggcagtt cgccagacgc
     4081 ggatcgcatt cctccatctc aatgcttgcc acgcgcgcta aattgccgcc gttagccacc
     4141 agcacctgac cgtcggcatc aaggaaacgc acaccgagcg cctgcgccat gcccataccg
     4201 ccgtcgaccg tcgcactgcc gccaatgccg agaataatat ggcgaatgcc gttatccagc
     4261 gcatgacgaa ttaactcccc cgtaccaaaa ctggaggcca gcaacggatt acgcttttca
     4321 ggcgcgacca gcatcaggcc acttgctgcc gccatctcaa tcaccgccgt tttcccgtcg
     4381 ccggtaatgc cataaaaagc attcactttt tcgcccatcg gcccgcagac ttcaagcgtc
     4441 acgatgttgc cgcccgtcgc ggcgaccatc gcatccaccg tcccttcgcc gccatccgct
     4501 atcggcaaac agatatagtt cgcatcggga aagagggtcg aaaacccggc tttaattgcc
     4561 tgacaacatt tttctgcact caagctctct ttaaaagagt ctggcgcaat gacaatcttc
     4621 attatcaatc ctttttcaac aaattattcg atgcgttccc acggaataag gttatccata
     4681 agaacagcca gtaaaatccc gaccaataag ccgttactga gcagaggacg aagcgttaat
     4741 ggaaggtctt gcagatacac aggcggtaat gccattaaaa atatgccgac aaataacggc
     4801 aatgcgagtc gataaatatt gcgagcggta aacgttattt gctggctaaa caccagcgcg
     4861 gaaaagagta aaggtagata agaaaccagc atgaccgcac tactcacggg taaagggata
     4921 ctgcaaaaca gtcgcgtgag tgccggaacc agcgccacca gcaggcaaat aacgctgcca
     4981 taaataaacg aacgccgcgt gtaatcgcca gtctgggtta ataaaccaat ggatgaaaca
     5041 aacggtgaaa atggaattac tgccagcggc acggttatca gtgtcataaa tccggtcgcc
     5101 acaaagctac gacgataacg cgtattcccc gcgccctgct gcggataaaa aacatccgtg
     5161 ccccgaatcg caccgtaggt attgctgata ttcaccagac ctgtaatcac ctccgtcaga
     5221 ataatccccg gcgacaaagc accgccactg ccgagcggga accactgcca gtgcaactca
     5281 ccggagagcg aatgcgaaga aggaaagcaa aagtaccaca acaaccagcc ggttatggtg
     5341 ccgaccagca ggccataacg ggcaaaacgt tgcggcagaa agataatcat cgccagtacc
     5401 aggcacatca ccgccaccga gagcgcgaac ggcggtaact gaattttaaa attcgggtcg
     5461 gctatgccaa acggcagccc gagcatacct ttgaaaaaga tagtggtcag ctgcgcgccc
     5521 agcatcaaca taaacaagac catcaccgac ggcgtaaata accgcgccag gcgatgacct
     5581 aatccgctaa aaccaatcaa catcgtcagc acgccggaga gtgcaatccc caccgccagg
     5641 ctggtagcga tatcgttgat cggtgtcccg cgcgatgctt cgccaagggt gatagttagg
     5701 atggttcccc accacaggcc ccccggccct tccataatag cgcgacgatg accgcaaaac
     5761 gcctgagcga agcaggccag tgcggtagca agaaaagcat attgcgtgag cgtaagcagg
     5821 ctactttgcg gcaactgaaa agcagaaagt agcgtaggag gaaccacaac cgtgttgcaa
     5881 aaaataaaga aaaaccactg aaatcctgat aacaggcttt cgcggctaac tgcaaaattg
     5941 aacataaaca caccttaacc ggagaatgcc cgccgcaagg acgggcattg caggggccag
     6001 attactgctg atgtttaagg ataaattgac ctttcggcgc aacagggaag ccctgttcga
     6061 tgtcgtaaat cacatcacca cgtaagatgg ttttcgtgat acgcgcgcca atggtacggc
     6121 caacatacgg gctgactttg tggcgatatt ccagatcgtc attggtaaga acatagctgc
     6181 tattcggctg aatgaagacg aagtcggcat cttttcctgg ggcgatacgg cctttttgct
     6241 gcagaccaaa aatatctgct gcgttagtcg ccattaattt gccgaacatt ggcagagaca
     6301 ttccgcgttt ctgtaccgct tcatcgaaca tcacgtccat acagttttgc agaccggcaa
     6361 taccgcccca tgcttccatg atgttgccgg ctttcatttc cggagggcat ggtgagtggt
     6421 cggaaaccag gcggtcgatt tcaccgttaa acagtttttc ccacatgcct ttctagtttt
     6481 ccagatcgcg gatcggcggt gaacacttcg ccagagtacc gatttcttcg aactgatcgg
     6541 tatccagtac gaagtaatgc gggcaggatt cacaagtaac gtcctgacct tcctgacgtg
     6601 cacgagtcac ttcttcaacg ccttccgggc tgctgatatg gcaaacgtgc agacggcaac
     6661 cggcaacttt cgccagatac agcacgcggc gaatcgcttc cacttcggta aataccggac
     6721 gcgaagccac atagtcatgg gcagttacgc gaccttcacg tttcgcttct tcgcccagtg
     6781 cgtcacagat cagcgcgttt tcgcagtgca ccagcaccgg ctgccccagt tcgcccagct
     6841 tctgcgcacc tttgaagaac tgccagtcgt ttacatcacg gaagtcgttg tcgataccgc
     6901 gatcgccaca ggtcgcaacg aagcatttga agccgacaac gcccacttca tccagctcat
     6961 gcagacggtc gatgttgtaa gacaccaggc caccgagttg tgccgcatcg atagtcagct
     7021 tgcctttagc ggcatcgaac ttcagttcaa ttgaagcgcg gtcaaccgtt gcaggcagct
     7081 ggttgagcgg catttcgatc atggtggtga taccaccttt tgccgctgcg cgagtaccgg
     7141 tttcataacc ttcccagtgg ctacgacccg gttcagaaat atgggtgtgc gcatcaacca
     7201 tgcccggcga aaccaccaga ccagacgcat ccataacttc ttttgcatcg cccagatcct
     7261 gaccgatagc agcaattttg ccgcctttaa cggcgatatc tacaacgcga gcttcgtttt
     7321 ctaaaataac ggtgccgttt ttaatgatta aatcaaaaga cataacaaac tccttgtaaa
     7381 atacagattg gtttttaact caagcaaagg cgacgatgac gccgacaaac catgaaacac
     7441 gcgataacgg ttccattaag ggaataaact taccgccaag agataaaata acggccacca
     7501 gagttactga aaacgcggtg aggttaaaac cgttatcgta atatttataa tcgccaggtg
     7561 cggtgtacag ttcatcaaga ttaatttgcc cacgcatcac cacaaaataa tgcgccatca
     7621 tgacaccaat taccggacca agcattccgc cgataatatc gaggaacaga taaatactgt
     7681 cctgattttc cattaatttc cacgggcaga tcagcaggct gataatactg gcaatcagta
     7741 cgccgttttt ataggtcagt tttgtcggtg caatggcagc aatctgataa ccagccggaa
     7801 taatattacc agtggcgtta gtggagatag tcgtcatcag aataaccagt accgcaaaga
     7861 acgaggcaaa caggctgtcc caacgctgaa caatatccag cacgttccag gtatcagcgc
     7921 cgtaatgaat actggctccg gcaataatac atacccccgc gaccgcaaac agaatatagg
     7981 ccacaactaa acccagcgtt tgccccagcg cttgctcacg aaacgagtgg gcgttttgcg
     8041 taaagtcgga tgcgctcacc gccggtgccg cccagaccgc aactaccgcg ttaatcacca
     8101 ccaggaacaa gaatccactg ttttctgctt tctgaatacc gctcggaatg tagtcaaaga
     8161 ttggaccgag cccgaccagc gaaatcgccc aaatcgccat accgccgaaa acgatataga
     8221 tgcacgggtt aagaatggca gtgaatttat ttaaaacttt gccaccgcca aaacctatac
     8281 caacgttgac cagccagaag agtaagaaag taattaagcc cggtagagaa aggcctaaca
     8341 gagtgaaatc accaccgaga gtcaaaaatc ccggccagat tttgccaatc agaatcaagc
     8401 aggccagtga ccccgcgtaa cattgcaggc caaaccacat aatggcagca ataccgcctc
     8461 ttaataatcc gggaaacagt gcgccacgta cgccgtaaga agcacgcagg atcatggcaa
     8521 acggtacgcc gtatttactg cccgcagcgc cgtttaatac cattaccgcg gcaatgaaaa
     8581 aggcgctgag gataattgcc agcataatac taaaggtaga caagccgaga ataaaaaagc
     8641 cgccgaccat cacataattg ggaacgttat gaaccgaacc catccataag gtaaaatagt
     8701 taaatgtttt ccaggtccgc tggctttgcg ttttcggcaa tagatcttcg ctatagccgc
     8761 gttgctggaa tagttttctc tgatgttcca taaatccctc ttaatttcac gtatcgaata
     8821 acgttgtttt aatctagcta tgaggaaaaa ataaaataaa aatattattt ttaatactga
     8881 agcaacatct ttggctgctt tcttttttaa tattgctggc cgattcattt tttgatatcg
     8941 gccattttta ttgcgggtat caggccagtt tatggttagc cattaattcc agcgcctgca
     9001 ctaacgcaga gtgatccaac tggctgccac cgtttgccgc gcaggtatta aataactcct
     9061 ggcaggtcgc agtgtttggc aggttcagag ccagtgcctt cgcactttgc agtgccaggt
     9121 tgagatcttt ctggtgcagc gcaattttga aacctggatt aaaggtgcgt ttaatcatgc
     9181 gctcaccgtg aacttccaga atacgtgagg aagcaaagcc gcccatcagc gcctggcgca
     9241 cacgtaccgg gtctgcaccg gcttttgaag caaacagcag agcttcagag accgcttcaa
     9301 tattgagcgc tacgataatc tgatttgcca ctttgcaggt ttgaccgtcg ccgttaccgc
     9361 ccacaagggt gatattttta ccgagcagtt caaacagcgg tttaacacgt tcaaataccg
     9421 cttcgtcacc gccaaccata attgacaacg tcccttcacg cgcgccgatt tcgccgccgg
     9481 agactggcgc atcgagataa tcaccaccca gttcattcac ctgacgagcg aaacgtttag
     9541 tttcaatcgg tgaaatggag ctcatatcaa caatggtttt gcccttcagc gaggctttgg
     9601 tacaaccatt ttcaccaaac agaacttctt caacctgagg tgtgtccggc accataataa
     9661 aaatgatgtc cgctgcttcc gttacctggc gcgcggtgtc gacattaaca gcaccgagtg
     9721 acagtaattc atcagcaacc ggtccaatgg tcgtgacatg taattgatga ccggcacgcg
     9781 ccagattaat ggccatcgga gtacccataa tgcctaagcc aataaatccc agtttcataa
     9841 ttaacctctt tttaaatgtc gctattcctg aattcagaca acacgatccc ctgccaactt
     9901 agcagcctaa aaaagcattg tctgaatagc gttacgtttt aacggtacgg atccatccag
     9961 cgtaaaccgg cttccgtggt ggtttggggt ttatattcac acccaaccca accgttgtag
    10021 tcagaatttt cgattacctt aaagagataa tcataattaa tttctccggt ccccggttcg
    10081 ccgcgatgcg gattatcggc aatttgcagg tgaccaattt tatcagccca ttgcgtcata
    10141 gtgttggtta attcaccttc catccgctgc atgtgataaa tgtcatactg aattttcaaa
    10201 ttgcagcaac caacatcatc aatcaatttc agcgcctgcc gagttccggt gagatggaaa
    10261 ccaggaatat caaaatggtt aataggttca atcagtaata aaatatcttc tttcatcagc
    10321 atatt
//
LOCUS       DBFIFM010000162        10271 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.162, whole genome shotgun sequence.
ACCESSION   DBFIFM010000162 DBFIFM010000000
VERSION     DBFIFM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.162"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            134..217
                     /gene="idlP"
                     /locus_tag="N7C74_003605"
     CDS             134..217
                     /gene="idlP"
                     /locus_tag="N7C74_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HGU2433404.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            214..606
                     /gene="iraD"
                     /locus_tag="N7C74_003606"
     CDS             214..606
                     /gene="iraD"
                     /locus_tag="N7C74_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HGU2433405.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSTLPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(599..1453)
                     /gene="hypT"
                     /locus_tag="N7C74_003607"
                     /gene_synonym="qseD"
     CDS             complement(599..1453)
                     /gene="hypT"
                     /locus_tag="N7C74_003607"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HGU2433406.1"
                     /translation="MTLEKCRNFSQAAVSRNVSQPAFSRRIRALEQAIGVELFNRQVT
                     PLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRKIKIAAAHSLSLGLLPSIIS
                     QMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLLEAPFDHIRLFESQLFPVCA
                     SDEHGEALFDLVQPHFPLLNYSRNSYMGRLINRTLTRHSELSFSTFFVSSMSELLKQV
                     ALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAYAYRMNTRMNPVAERFWREL
                     RELEIVLS"
     gene            complement(1575..2747)
                     /gene="iadA"
                     /locus_tag="N7C74_003608"
     CDS             complement(1575..2747)
                     /gene="iadA"
                     /locus_tag="N7C74_003608"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HGU2433407.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIVRAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            complement(2760..3221)
                     /gene="yjiG"
                     /locus_tag="N7C74_003609"
     CDS             complement(2760..3221)
                     /gene="yjiG"
                     /locus_tag="N7C74_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HGU2433408.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            complement(3218..3901)
                     /gene="yjiH"
                     /locus_tag="N7C74_003610"
     CDS             complement(3218..3901)
                     /gene="yjiH"
                     /locus_tag="N7C74_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HGU2433409.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            4151..4701
                     /gene="kptA"
                     /locus_tag="N7C74_003611"
                     /pseudo
     CDS             4151..4701
                     /gene="kptA"
                     /locus_tag="N7C74_003611"
                     /inference="COORDINATES: protein motif:HMM:NF014001.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
     gene            complement(4858..10099)
                     /gene="iatD"
                     /locus_tag="N7C74_003612"
                     /pseudo
     CDS             complement(4858..10099)
                     /gene="iatD"
                     /locus_tag="N7C74_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168882.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter IatD"
BASE COUNT         2463 a         2533 c         2594 g         2681 t
ORIGIN      
        1 atttttatca aatcatgact tttttcctga aaagtcagta gatattcata ggcaagtaag
       61 gttttatact ttgctgacag gattcaggcc tgtctcagac tgacatggat gtaatgaaca
      121 aaagggaatg gctatggaaa atgagcatca atacagtggt gcccggtgtt cagggcaagc
      181 cgcatatgtt gctaaacgtc aggagtgcgc aaaatgatgc gccaatcact tcaggctgtt
      241 ttacctgaaa tttcaggcaa taaaacgtca ttgctgcgta aatcggtatg cagcgatctc
      301 ctcactcttt ttaattctcc tcattcgaca ttaccgtcat tacttgtttc aggcatgccc
      361 gagtggcaag tgcataaccc gagcgacaaa catctccaga gctggtattg ccgtcagctg
      421 cgttctgcac tgttatttca tgagccacgg attgcggcgt tgcaggttaa tttgaaagaa
      481 gcgtactgcc acactctggc gatcagcctt gagataatgc tgtaccacga cgatgaaccg
      541 ctaacgtttg atctggtctg ggataatggc ggttggcgca gtgcgacgct tgagaatgtc
      601 agctaagcac aatctccagc tcgcgcagtt cacgccagaa gcgttcggca actggattca
      661 tgcgggtatt catccggtat gcgtaggcct gaatcgggat caccagttcg tcccgattca
      721 gcacaacgag ctgcccgctg cgaatttctt gttggatggc atactccggc agccaggcaa
      781 tcccacagcc gtcgagggca acctgcttta aaagctcgct catcgaagag acaaaaaagg
      841 tgctgaaact taactcactg tggcgcgtca gggtgcgatt aatcaatcgc cccatgtagg
      901 agttgcggct gtaattcagt aacggaaagt gtggctgcac gaggtcaaaa agtgcttctc
      961 catgttcgtc actggcgcag acagggaaca attgagattc aaataagcga atgtggtcaa
     1021 acggcgcttc cagcaaatct tcgtcgtgaa aggaaaaaat acagtcactt tgtccttcac
     1081 gcagtttatc gaccgcttca tcgacatcaa tggcttcaat cgcccaggta aagagcggcg
     1141 gcatctggct gataatggac ggtaacagac cgagggaaag agagtgtgca gcggctatct
     1201 tgattttacg ttgcgcgtaa tcgctgccgc cacgcagctc tgcaaggttg ctttctaact
     1261 gttgcaacag atggcggatc tgcgaatgga agattttccc ttgttctgag agttgcagcg
     1321 gcgtcacctg gcggttaaac aattcaacgc caatcgcctg ttccagcgcg cggatgcggc
     1381 ggctgaatgc cggttgcgag acgttacgac tgactgccgc ctgggaaaaa ttgcggcatt
     1441 tttccagggt cagaaaatct taaagccatt tggtttcaat attatgcaaa accgcaccac
     1501 agtcatccat tacgttttac tctgtcagcc gcccttgggg gcattctacg tccattaggg
     1561 cggctgacaa ccgtttaagc cgtttcaaac gttcctttca cgcaggcttt gccgtctttg
     1621 accatcagtt tgccgcgagc gtatacctgc tcaatgcgca attctggcgt catgaccagt
     1681 aagtctgcgt cattgcctgg cagaatttcg cctttcccgg tcaggttaag gaaaccggct
     1741 acgctactgg tgagcgggcg cagggcatcg ctgatactga aatcatagtc tttaaccagc
     1801 acctgcacgg tttccagcaa cgtttcaaaa cctgcaacgc cgatatgggt taaattccct
     1861 tcatcatcga agaacggctg gctaccgttg ccgtcggagc tgagggtgac gcgtgccagc
     1921 ggaatacccg cctgaacggc gcggacaata ccttcggcag gggcgaccgg ttcgtcaatg
     1981 ctgctggtga tatcgatggt gccgcctttg cgcgcgaact ccagcgcctg ctcaaacaac
     2041 ggtacgttgc ggttaacgtg ggtcggcagc agcttgctga tcggcacatc gcagttttcc
     2101 agcaggtcat aaataggctg taatgccttt ttactgtcgc ccatgtggaa cacggtgacg
     2161 ccaggtttac cgccgagcaa accgccaacg cgggattctg ccgccatatt agccaggtga
     2221 taaacgtccg gtgcggcaga acggtgatca gagatggcac atttcacgcc aatcacacga
     2281 tcgataatcg ccacgtcttt ttccacggaa cccgtaatgg tgcgggaagg gacatgataa
     2341 gcgccggtca gcatccaggc gctgatgcct tcttcattga gcgcacgggt cttggcgagc
     2401 agggattccg ggtggcgaga gatagagtcg gtgcccagca gaccaaccac tgacgtgacg
     2461 cccgcttccg tcaggcgact tagcgccact tccggcgtgc gcgtcgtggg acctgcttcg
     2521 ccgccaccgc caatcaaatg gacgtgttga tcaataaaac ctgggcagag gatctgcccg
     2581 ctgagatcga caaccgtgca gtccggtaca atgtcagaag ggatattact ggcaacggcg
     2641 ataattttgc cgttagcgac gaggacatcg caaattcccc gatcttccgg cgcatacaaa
     2701 tgcgctccct gcagcagggt aaaaccggct gcggtataat caatcatggt aactccttgt
     2761 taaacaataa gctgcataac ccagatcgac agcaatgcgt taatcacgca gaccgtaatg
     2821 atatgcgggt aatatttggc attcacttcg gctgtcccca ggcagcgacc gacattttgc
     2881 accgggttgc ccattaaata catcgcgggc agtaaaacgg tgacatcgtg accggttaat
     2941 gcgccagcgg ttgccagact ggcggcaaca ccgactgcgc cgcccatact cattaatgcg
     3001 gctaaaagga cggttgccgc ttcaccgggt aatccccaca gcgccattac gggttcacag
     3061 atatgcccca cccagtcgag caggccggta attttcagcg cctgaatgat gacaaacgcc
     3121 atcaccacgt ttggcagcag gttggtcgtg gcgatagtaa agccacggcg cgcgccatcg
     3181 ataaacatat ccatgacgtt tttgcggacc tgagttgtca tgcttgcgct ccttgcgttg
     3241 ggttgcgacg ttcttcgaag ttaagccaga cgcgcaaaat gttggctccg acaaatttaa
     3301 acaccaggat gacggctaaa ggtacgatta cggacgtgcc cagaaaggcg aacaccgcga
     3361 cgccggaaga gaagtagttg gtgatgatgg cgctaccgct ggtttgatat gcggcgaaaa
     3421 tcactttatc gcgttcggta atttcacctt cctgcgccag ctctttggtc ataccggcgg
     3481 cggcatcagt gttttgcaag ttagcgatca gcgccagcga gcagatccct ggaatgccca
     3541 acaacggttt tagcacaggt gtcattaact gctgcgcggc gcgtaatcca ccaagaccat
     3601 cggtaattga aataatcccc agagagagaa tgactgacgg tgccagctcc agcgcaaaga
     3661 gaaagccatc tttagccccc gcgccacccg cgccgcgaaa tgaggtggtg gctccattcg
     3721 caccaggcag ttgcccaaac gatccgttca gaacggaaaa atcaaaaacg cgccaccagc
     3781 tgtctgttcc ggaaaaaaca ccggaaaaaa agattatggt gagaaaaaaa gccagatacc
     3841 ctttaatgcc cactttttct gtcgccagct ccccggctac agcatcccct tgttgtgtca
     3901 taacaatccc cattgttttt ttgtgtttgt gtgttgcttg agccaggaaa attcctggaa
     3961 cttaaaaacc ttatcagcca tgtgggtatg gggaaaatgc aagtatgttt ctggctatgc
     4021 gttttttgca taggggaaaa attacgctgt gtggaaaagg ttctgcgcag gattgagttg
     4081 cagaaaattc ataaacccag tgacaatgac attggtaaat aaaaacagaa aatccaggga
     4141 cgaaaacgcg atggcaaagt acaacgaaaa agagctggcg gatacgagca agtttttaag
     4201 ttttgtcctc cgacacaaac cggaggcgat tggtatcgta ctggaccgtg aaggatgggc
     4261 gaatatcgat aagctcatac tttgtgccca aaaagcgggc aagcgattaa cccgtgcatt
     4321 gctggatacc gtggtcgcaa ccagcgataa aagcgtttta gttattccag tgatggtagg
     4381 tgcatccgcg cggttcaggg gcattcgact tcgcaggtcg ctatttcctt tgccgaaaaa
     4441 acaccgccac agtttctcta ccacggtacg gcaagccgtt tccttgacga gataaaaaaa
     4501 caggggctga ttgcagggga tcgtcattac gtgcatctct ctgctgatga agcaacggca
     4561 cgcaaagtgg gagcaagaca cggatcgccg gttgtcttaa ccgtcaaagc acaggaaatg
     4621 gcgaaacgag gcatcccttt ctggcaagcg gaaaacgggg tctggctgac atcaacggtt
     4681 gctgttgagt ttcttgagta gtcgtttatg ccggctgggg tacaaaaaca ataaattcaa
     4741 tatattgcag gggttatata ggcctgataa gcgcagcaca tccggcaatg tagcgtttgt
     4801 tatcagtctg aaaggatacc ctcgtaaaag ggtatccttt aataacaact gctgaaatta
     4861 aagtgattta cggcaggcgg tcagttgggg gctggaatca gcgtcgcttc cggtcatacc
     4921 ggttagcatc tgcacatagt agtgccgact gtaatcatca gattcacttg tccagtaagc
     4981 tcccggtgta aattgcgaat tactggaaaa cgctccaacg ttcccccatt caccatacag
     5041 tgatccctgc gtgcgtttgt tttcgccaga cgtcaaatgt gtgatgccag gaagattata
     5101 tccatcattt ttagcatgac agagcgcatt ggcagctagc cagatattgg tatgatttcc
     5161 attattcacc caccagcctt tcagcttaat taaggtctgg taggttgtac ctccttgtcg
     5221 gggaacagcg cttattgtga ctgacgtttg atcgccgata tttgcgaaag aaactttacc
     5281 cgaagcatcg acagacaccc aaccctgtga acttgaaaac atataatcat caacggtttt
     5341 acccgcttca aagttatcat tgttgagtgt gtaatacgct cctgcaaagc cttcgctcgg
     5401 gaatttagcc gtggagaagg tatggttgtt tgcagtgaca tgaccggaca tttcttttat
     5461 cgattggatg agattcagcg atatgcttaa gtttgcctga tgaaccccgt tcagcatggg
     5521 aatgagtgtc gctgttccct cttttccgcc agtgacagtc acggtgtact cgccataatt
     5581 tctttggctg taatcaatat cgctcaactt gagaaagttc acgaaggggc ctgactggac
     5641 aaattccaga tgatcagacg tcactatcgg gttaccgtac ttgtcttgca atgttagcgt
     5701 aagttttgcg ctctgctggt agtcagcctt cagagtgtca acatcggatt tcaaaattga
     5761 cttactttca tcggccgtgc cggcaaccaa tgtaatggtt gcggattggt tggctgtgcc
     5821 aggaacttgc acattgactg ttaaggtttc tgccaatgta ctggttattg tcgccgtgac
     5881 ttgcccggac tgatcggtac gcgtagttgg aatattaact accgggctgc ctttggtgtc
     5941 taagccatga ctaatatcga caccttcaac cgggaatccg taatcatctt ctactcgtga
     6001 agtgatggtg ctgctatcga tgccgtcggc ggtgatggtg tcaggagaag ccacgacgct
     6061 cacaacatgt gccgttttag catctgcctt aaccgcaacc gttacctggc tctgaacggg
     6121 aacatctttg cccattatgg ccgtaacggt atagacattc actttgtcac tggtcagcgt
     6181 cactttggcc tgacctgact ggtcagtaag gacttcccct gtattgctta gcgttgcacc
     6241 ttcaggcgtt acgctaaagg tgataatttg ccctggcatc aattcacccc gcgcatttct
     6301 cgccgttgcg gtcagcgtaa taccctcact attatggacc gtagtttctg gtttcgatga
     6361 tgttaactcg agtttgccat ttgccacatt cacgtcaacc ttactactac tgttattgtc
     6421 cagcgttgcc gtcacctgat aaaccccagg ttgggtgctg agaaggttaa cgtacgcata
     6481 accgttatta tcggtgtaag ccgtatcgcc tatcgtaatg ttagttgttg gctgcgcact
     6541 taatctgacg cgttgacccg cgattacatt gttaaacgcg tcctggacga gtatccgtag
     6601 cgtgatgttt tcgcccaccg ttatcgtttt ttcggctccg gttatactta ttaccttcgc
     6661 cgaactttca tccggtagca ctgataaact aaacgtttta ttatcggaag cttgcgcttt
     6721 ccggctcacc acgacgttat gttgtccggc aatattgctg gtcattgtta ccgtcgcctg
     6781 acccagcgta ttggtggtga cttctgtttc gcttagagtg acatcgttac tctggaaagt
     6841 gaccaactgg tctttaagcg gattttggta agcgtccagg atcgttgccg tcagggtact
     6901 ccccgtactg ttacccgcaa caatggaatt taatgaggca gtcagcggct ggagctgcgc
     6961 actgtttgta tccccaataa aagtgacttg ctgataagca acggtattat ttctggtcag
     7021 cgtcgccgta actgtaaact cacccgcttt tgtgcctcgc aatgtgacca cggcatcacc
     7081 gtttgcatcg gtgcgggcgg tttcactact tagcgtgaag ttcccggtca tggaggctgg
     7141 caaggtaaat ttcacatcct catccggcaa cgggtgtcca taaacatctt tcacactggc
     7201 agtcagggta acgatatttg tattgttagc gatcgcactg cccgacccgg cattcaaggt
     7261 cattaccgat tgttccctgt ttgggaccac ggtgatcgtg accgactggt gaatattttt
     7321 atccacctca agcgaggcat cgacggaata gctaccagct tttgaactgg tcaggtgcac
     7381 ccgcacaata ccggactggt ctgttgtggc ggtcgtgttg tccagttgcg agccaccagg
     7441 tgccacaaaa ttcactttca tatcttttaa cgggttgtta tgaaagtctg tgatgatggc
     7501 ggtcagagta ctgccctggc tgttgccagc aataatggta tctggatcgg caaccagttg
     7561 tttgaatttt ggcgtactcg aatcgctaac gaatttcgca attatccgtt gtgaaaaacc
     7621 atttggcagc gtggcgctaa tcgtattttc ttccgcaacc tgacttacca tgtttattgt
     7681 ggcaacacca tatgcatccg tattttgctg cggcggttcg acaaactccg cgcttccttt
     7741 ttcattctta aaggtgacct gatgatttgt caggggatta tcactctcat cagtgacgtt
     7801 gatcgtgagg gtattaaatg tctttttatc ggcgtaaacg tcattatttg tcgctgataa
     7861 tgtggcaacg cgagccttat tctggtttgc ctcaatgtta taaatatgcg attgcagtgg
     7921 agcattccag ttgtgaaggc taagttgtgc ccttagtgca gtcccttgct tataggccgg
     7981 ataattcgcg gcataaaccc ccggctgctc ttcgtgccag acaatcgtgg cgccaggttt
     8041 gctgttttcg acagtaacgg cttttaccaa ttcctctttt tgatatgcaa ccggttgatt
     8101 gctatcgtct ttcagttcca ccctaacctt gatgtcgtct ccggcataat acgatacgtt
     8161 atcaattgtt attgatgaat ggtcgcggga tgagacaaca gggacgatat taacgacgat
     8221 ggattctttt accgcactct caccgttaat ttgcggcgtc agtgttactg aacctgatgt
     8281 cgttccggcg gtcagtatct gtgtgtaact accatcaccg ttatctgtcc aatcagacag
     8341 ggtgatatcc tgaacgcctt cactgcgggt ttgcagcgcc agccccggca ccggagttcc
     8401 gtcggaatcg tgcgcagtaa cggtcaatgt ggtcgtcgat tttttatctg cagccacggt
     8461 tagcggattg acggataaca gagaatcttt ctggcttaat gtcggagcct gaataactac
     8521 catgctttgt tcatgccgtg acaggttacc ttttacatcc tcggcggtaa cgtctatcga
     8581 ccatgtatta tcggtttctg gggtgttagt gaagcggtaa cctggcagcg tgaccgtgat
     8641 atcttttcca gacgtgctga ctttacctcc ggcagcttcc agcgcagcgg cttcgatgtt
     8701 atagccttta agggcatatt tggtctgtag cgaggaaacc agcggtttta tttctccaga
     8761 cttccctttc accggatcca gcagactcag gcgaatcagc tctttcttgc ggtattccag
     8821 aacgatgttg ttgttgcgat caatcaggtc ataacgacta ccggccagac tgcgccgtcc
     8881 ggccacttcg tccggattaa gctgtttctg cattgaactg ctgggttgcc aggtcagatc
     8941 aacggcaaaa cgtgtgtcat tttcaccttg cttcccctga cgctgttccg cactgagagt
     9001 cagaagcggg aagggggtat agttgaggcc tgcggtaata gcatgggggt tactttgacg
     9061 atcattcttg tcaaacagcg ccacttcatc gccatagtat tgttcataga ccagttttcc
     9121 ccccagttgt ggccaggcag gtaaccagcc ttccgcgcgt aaatcccagc cgttggccgg
     9181 gcgggcttcg aagtcgttat ccaattctgg tgcgctacgc cagccggtca ggccgatata
     9241 agcgttgctg ctcaacttca gataatcacg ccagtattct gcgccaagcc ctgcgcggga
     9301 gtgatagcgg ctcaggtcgt ggtcaaaaaa aaggttgatg cctgacatcc agctggaggt
     9361 gaaatgacgc cagcccaaac cggtgttgat ctgggtacga tcgtctgttc ggtgaagggt
     9421 atgctggctg aagagcagat aatcaggtgt gtcataccac ggatgcagga agtcgaattg
     9481 cgaatttttc aggctaaaat cttcatccac acccagagag attctcgcag taccaaagtt
     9541 atttaaccaa tcagtcatcg cgcctgaggc ttctgaagac gcccaaccac gcgccatgcc
     9601 gctggcctgc tcactattca tatcttgtga aagcagagtg ccaactcgct gcgaggtgct
     9661 ggctatttga ttctccagcg tgttttcgcc attcgcaggc ggtacggcat tttgttgctc
     9721 atggcttttc tgcgaggttg ttgccggaac atccagttct tcaccctggc gcacgttatc
     9781 aaagccgcga gcaaaagtac ggaactgatt aagacgacga agctcctcca gtgaaatacc
     9841 gaagcgatta gcaacgcttt gcgctgattc cagcgcacca agcgtgtaag gcacagtatt
     9901 attttctagt agaggggggg gcagatgaaa ctgtcgtctg aggtttagca tgtaccacat
     9961 tttgtgccgt cgccatgacg gggaaaaaaa attgtgtaaa taaacaaact ccggcggtca
    10021 ggcggcgtat tttgtcaccc agaccacata aagagttttg atcattattt tttatttgat
    10081 gcgcatttgt cgaagccata gtgaccttcc gtgaaaaaac agaattaaac ttgttttttc
    10141 tagcacggag atataatgcg atcctgacta taatatgacc agaaataatg aattagccgt
    10201 ggaattgcta aaccaggaat aataccggcg gtggctgagt acctccaaag aaaaaggata
    10261 ttttaattat t
//
LOCUS       DBFIFM010000163        10253 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.163, whole genome shotgun sequence.
ACCESSION   DBFIFM010000163 DBFIFM010000000
VERSION     DBFIFM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.163"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..570
                     /locus_tag="N7C74_003613"
     CDS             <1..570
                     /locus_tag="N7C74_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU2433410.1"
                     /translation="KTGAEAIRALATQLPVFRQKLSDGWYQVRITGRDVSTSGLTAQL
                     HETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGMT
                     GILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLYG
                     EMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            631..4029
                     /locus_tag="N7C74_003614"
     CDS             631..4029
                     /locus_tag="N7C74_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HGU2433411.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQKVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGASFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDLSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRIADIRQVETSARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTSTYASGTITVRISDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMP
                     AGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            4096..4695
                     /locus_tag="N7C74_003615"
     CDS             4096..4695
                     /locus_tag="N7C74_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU2433412.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGMAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            4760..7675
                     /locus_tag="N7C74_003616"
     CDS             4760..7675
                     /locus_tag="N7C74_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HGU2433413.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNAAVSQQSA
                     ATSASTATTKASEAASSARDASASKEAAKSSETSAASSASSAASSATAAGNSAKAAKT
                     SETNAKSSETAAEQSASAAAGSKTAAALSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKSSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYELANGKYTAQDATTA
                     QKGIVQLSNATNSTSEMLAATPKSVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSA
                     SETLAATPKAVKAANDNANGRVPSARKVNGKALSSDITLTPKDIGTLNSTTMSFSGGA
                     GWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQ
                     RTSTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPE
                     GLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSG
                     VIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTN
                     NTGAHTHSVSGTAASAGNHTHSVTGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHS
                     VSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            7675..8256
                     /locus_tag="N7C74_003617"
     CDS             7675..8256
                     /locus_tag="N7C74_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU2433414.1"
                     /translation="MAFRMSEQPRTIKIYNLLTGTNEFIGEGDAYIPPHTGLPANSTY
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPVIPEV"
     gene            complement(8376..9266)
                     /locus_tag="N7C74_003618"
     CDS             complement(8376..9266)
                     /locus_tag="N7C74_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HGU2433415.1"
                     /translation="MFRHVKKLQYTVRVSEPNPGLANLLLEQFGGPQGELAAACRYFT
                     QGLSDEDAGRRDMLMDIATEELSHLEIIGSLVGMLNKGAKGALAEGTENEAELYRSLT
                     QNGNDSHITSLLYGGGPALTNSAGVPWTAAYVDTIGEVTADLRSNIAAEARAKIIYER
                     LINVTDDPGVKDTLAFLMTREAAHMLSFEKALHSIRNTFPPGKLPPLEKYKNVYYNMS
                     EGEDVRGSWNSDENFDYVSDPVSAVDGGDGKASINLSTKQEAMIKAMATRLKSHEDIN
                     PVTGAELAEGEPQTKINSKN"
     gene            complement(9285..9791)
                     /locus_tag="N7C74_003619"
     CDS             complement(9285..9791)
                     /locus_tag="N7C74_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGU2433416.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAEKMLESMASRIENYPELRSRIEQ
                     HISETKNQLSQLESILDRNNISRSVIKDSMSKMAAFGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASVPIIEAILNEEKQMAEWLLNHIPDTTEQFMVRSE
                     IDGVEAKK"
     gene            complement(9828..>10253)
                     /locus_tag="N7C74_003620"
     CDS             complement(9828..>10253)
                     /locus_tag="N7C74_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGU2433417.1"
                     /translation="KALSKLSRSAYSDKLTAAFQSHLDETHGQIERIDQVVDSEDGLK
                     LKRIKCAAMEGLIEEANEVIESTDKNEVRDAALIAAAQKVEHYEIASYGTLVTLAEQL
                     GYKKAAKLLKETLEEEKATDVKLTDLAFNNVNKKAQDNS"
BASE COUNT         2582 a         2512 c         2825 g         2334 t
ORIGIN      
        1 aaaacggggg ctgaagccat ccgcgcactg gccacacagc tcccggtgtt tcgtcagaag
       61 ctgagcgacg gctggtatca ggtacggatt accgggcggg acgtcagcac gtccgggtta
      121 acggcgcagt tacatgagac tctgcctgat ggcgctgtga ttcatattgt tcccagagtc
      181 gccggggcca agtcaggtgg cgtattccag attgtcctgg gggctgccgc cattgccgga
      241 tcattcttta ccgccggagc cacccttgca gcatgggggg cagccattgg ggccggtggt
      301 atgaccggca tcctgttttc tctcggtgcc agtatggtgc tcggtggtgt ggcgcagatg
      361 ctggcaccga aagccagaac tccccgtaca cagacaacgg ataacggcaa acagaacacc
      421 tatttctcct cactggataa catggttgcc cagggcaatg ttctgccggt tctgtacggt
      481 gaaatgcgcg tggggtcacg cgtggtttct caagagatca gcacggcaga cgaaggggac
      541 ggtggtcagg ttgtggtgat tggtcgctga tgcaaaatgt tttatgtgaa accgcctccg
      601 ggcggtttta tcgtttatgg agcatgacga atgggtaaag gcagcagtaa ggggcatacc
      661 ccgcgcgaag cgaaggacaa cctgaagtcc acgcagctgc tgagtgtgat cgatgccatc
      721 agcgaagggc cggttgaagg tccggtggat ggattaaaaa gcgtgctgct gaacagtacg
      781 ccggtgctgg acagtgaggg gaataccaat atatccggcg tcacggtggt gttccgggcc
      841 ggtgagcagg agcagacacc gccggaggga tttgaatcct ccggctccga gacggtgctc
      901 ggtacagaag tgaaatatga cacgccgatc acccggacca tcacgtcggc aaacattgac
      961 cgtctgcgtt ttactttcgg cgtgcaggca ctggtggaaa ccacctcaaa gggggacagg
     1021 aatccatcgg aagtccgcct gctggttcag atacaacgta acggtggctg ggtgacggaa
     1081 aaagacatca ccattaaggg taaaaccact tcacagtatc tggcctcggt ggtggtggat
     1141 aacctgccgc cgcgcccgtt taatatccgg atgcgcagga tgacgccgga cagcaccaca
     1201 gaccagctgc agaacaaaac gctctggtcg tcatacaccg aaattatcga tgtgaaacag
     1261 tgctacccga acacggcact ggtcggcgtg caggtggact cggagcagtt cggcagccag
     1321 aaggtgagcc gtaattatca tctgcgcggg cgtattctgc aggtgccgtc gaattataac
     1381 ccgcagacgc ggcaatacag cggtatctgg gacggaacgt ttaaaccggc atacagcaac
     1441 aacatggcct ggtgtctgtg ggatatgctg acccatccgc gctacggcat ggggaaacgt
     1501 cttggtgcgg cggatgtgga taaatgggcg ctgtatgtca tcggccagta ctgcgaccag
     1561 tcagtgccgg acggctttgg cggcacggag ccgcgcatca cctgtaatgc ctacctgacc
     1621 acacagcgta aggcgtggga tgttctcagc gatttctgct cggcgatgcg ctgtatgccg
     1681 gtatggaacg ggcagacgct gacgttcgtg caggaccgac cgtcggataa ggtgtggacc
     1741 tataaccgca gtaatgtggt gatgccggat gatggcgcgt cgttccgcta cagcttcagc
     1801 gccctcaagg accgccataa tgccgttgag gtgaactgga ttgacccgga taacggctgg
     1861 gagacggcga cagagcttgt ggaggacacg caggccattc tccgttacgg tcgtaacgtc
     1921 acgaagatgg atgcctttgg ctgtaccagc cgggggcagg cacaccgcgc cgggctgtgg
     1981 ctgattaaaa cggagctgct ggagacgcag accgtggatt tcagcgtggg cgcagaaggg
     2041 cttcgccatg taccgggcga tgtcattgaa atctgtgatg atgactatgc ggggatcagc
     2101 atcggtgggc gtgtgctggc ggtgaacagc cagacccgga cgctgacgct cgaccgtgaa
     2161 atcacgctgc catcctccgg taccacgctg ataagcctgg ttgacggaag tggcaatccg
     2221 gtcagcgtgg aggtccagtc cgtcaccgac ggcgtgaagg taaaagtgag ccgtgttcct
     2281 gacggcgttg ccggatacag cgtatggggg ctgaagttgc cgacgttgcg ccagcgcctg
     2341 ttccgctgcg tgagtatccg tgagaacgac gacggcacgt atgccatcac cgccgtgcag
     2401 catgtacccg aaaaagaagc catcgtggat aacggggcgc actttgacgg cgacctgagc
     2461 ggcacggtga atggcgtcac gccgcccgcg gtgcagcacc tgactgccga agtcaccgca
     2521 gacagcgggg aatatcaggt gctggcgcgc tgggacacgc cgaaggtggt gaagggggtg
     2581 agcttcctgc ttcgcctgac cgtggcagcg gacgatggca gtgagcggct ggtcagtacg
     2641 gccaggacga cggaaaccac ataccgcttc acgcaactgg cgctggggaa ctacaggctg
     2701 actgtccggg cggtaaatgc gtggggacag cagggcgatc cggcatcggt atcgttccgg
     2761 attgccgcac cggcagcgcc gtctcggatt gagctgacac caggctattt tcagataacc
     2821 gccacgccgc atcttgcggt ttatgatccg acggtacagt ttgagttctg gttctcggaa
     2881 acgcggattg cggatatcag gcaggttgaa accagcgcgc gttatcttgg tacggcgctg
     2941 tactggatag ccgccagtat caatatcaaa ccgggccatg attattactt ttatatccgc
     3001 agtgtgaaca ccgttggcaa atcggcattc gtggaggccg tcggtcgggc gagcgatgat
     3061 gcggaaggtt atctggattt tttcaaaggc aagataaccg aatctcatct tggtaaagag
     3121 ctactggaaa aagttgacct gacggaggat aacgccagca gactggatga gttttcgaaa
     3181 gagtggaagg atgctaacga taagtggaat gccatgtggg gcgtcaaaat tgagcagacc
     3241 aaagacggca aacattatgt cgcgggtatt ggcctcagca tggaggacac ggaggaaggc
     3301 aagctgagcc agtttctggt tgccgccaat cgtatcgcgt ttattgaccc ggcaaacggg
     3361 aatgaaacgc cgatgtttgt ggcgcagggc aaccagatat tcatgaacga cgtgttcctg
     3421 aagcgcctga cggcccccac cattaccagc ggtggaaatc caccggcatt ttccctgaca
     3481 ccggacggaa agctgactgc taaaaatgcg gatatcagtg gcagtgtgaa tgcgaactcc
     3541 gggacgctca acaacgtcac gattaatgag aactgtcaga ttaaggggaa actgtcagcc
     3601 aatcagattg aaggcgatat tgtcaaaacg gtcagcaagt ctttcccccg cacgagcact
     3661 tatgccagtg gcaccatcac ggtaagaatc agtgatgatc agaaatttga ccggcaggtc
     3721 atgataccgc cagtgttatt ccgcggtggt aagcatgaga atttcaacag taataaccaa
     3781 cagtcatact ggtattcaac ctgccggtta agagtgaccc gcaatggcca ggagattttt
     3841 aatcagtcca cgacggatgc tcagggcgta ttttcctcag ttatagatat gcctgccgga
     3901 caggggacgc tgacactgac attcaccgta tcttcatcag gagcgaataa ctggacacca
     3961 acaaccagta tcagcgatct gctggttgtg gtgatgaaaa aatccacagc aggtatcagt
     4021 atcagctgaa ttttataacc cagaacgggc gtcagaaatg acgccttttt tattgcagaa
     4081 aagcgagagg taattatgcg taaactttat gccgccattt tgtccgcagc catttgtctg
     4141 gccgtatccg gtgcgcctgc atgggcatct gaacatcagt ccacgctgag cgcggggtat
     4201 cttcatgccc ggaccaacgt tcccggcagt gatgatctga acgggattaa cgtgaaatac
     4261 cgttatgagt ttacggatac gctggggctg gtgacgtcat tcagctatgc aggagacaag
     4321 aatcgccagc tgacccgtta cagcgatacc cgctggcatg aagattccgt gcgtaaccgc
     4381 tggttcagcg tgatggcggg gccgtctgtg cgcgtgaatg aatggttcag cgcgtatgcg
     4441 atggcgggta tggcttacag ccgtgtttcg actttttccg gggattacct tcgcgtaact
     4501 gacaacaagg ggaaaacgca cgatgtgctg accggaagtg atgacggtcg ccacagcaac
     4561 acgtctctgg cgtggggggc tggcgtgcag tttaacccga ccgaatccgt ggccattgat
     4621 attgcttatg aaggttctgg cagtggtgac tggcgcactg acgggttcat cgtgggtgtt
     4681 ggttataaat tctgattagc caggtaacac agtgttatga cagcccgccg gttcaggcgg
     4741 gcttttttgt ggggtgaata tggcagtaaa gatttcaggt gtactgaaag acggcacagg
     4801 aaaaccggta cagaactgca caatccagct gaaagcaaaa cgtaacagta ccacggtggt
     4861 ggtgaacacg ctggcctcag aaaatccgga tgaagccggg cgttacagca tggacgttga
     4921 gtacggtcag tacagcgtta ttctgttggt ggaagggttc ccgccgtcac atgccgggac
     4981 catcaccgtg tatgaagatt ctcaacccgg tacgctgaat gattttcttg gtgccatgac
     5041 tgaggatgat gtccgtccgg aggcactgcg ccgttttgag ctgatggtgg aagaggtggc
     5101 gcgtaacgcg tccgcggtgg cacagaacac ggcagccgcg aagaagtcag ccagcgatgc
     5161 cagcacatca gcccgtgagg cggcaaccca tgcgactgat gctgcggact cagcacgcgc
     5221 agccagcacg tcagccggac aggccgcgtc gtcggctcag tcagcgtctt ccagcgcagg
     5281 aacggcatca acaaaggcta ctgaagcatc aaaaagtgct gccgctgcag agtcctcaaa
     5341 aagcgcggcg gctaccagtg ccggtgcggc gaaaacgtca gaaacgaatg cggcagtgtc
     5401 acaacaatca gccgccactt ctgcatccac cgcgaccacg aaagcgtcag aagctgcctc
     5461 ctcagccagg gatgcgtcgg cttcaaaaga ggcggcaaaa tcatcagaaa cgagcgcagc
     5521 ctcgagcgcc agtagtgcag cctcctcggc aacggcggca ggcaattccg cgaaggcggc
     5581 caaaacgtct gagacaaacg ctaagtcctc tgaaacggca gcagaacaga gtgcctccgc
     5641 agcagcaggc tcaaaaacag cggctgcatt atctgccagt gccgcgtcaa caagtgccgg
     5701 gcaggcctca gccagtgcca ccgccgccgg aaaatcggca gaaagtgccg catcgtctgc
     5761 ttcaacagcc acaacgaagg ctggcgaagc cactgaacag gccagcgcag cagcgagttc
     5821 tgcttccgca gcgaagacat ccgaaacgaa cgcgaaagcg tcggaaacca gcgcagaatc
     5881 ctcaaaaacg gctgccgcat cgtcagccag ttcggcggcg tcatcggcat catctgcgtc
     5941 tgcttcaaaa gatgaggcga ccagacaagc gtcagcagcg aagagcagcg ccacgacggc
     6001 atccacgaag gcgacagagg cagctggtag tgcgacggca gcagctcaga gcaaaagtac
     6061 ggcggaatct gcagcaacgc gcgctgagac agcggcaaaa cgggcagagg atattgcatc
     6121 cgccgtggcg cttgaggatg cgagcacgac gaaaaagggg atagtacagc tcagcagtgc
     6181 gactaacagc acttccgagt cactggcggc aacgccaaaa gccgttaagg ccgcgtatga
     6241 gctggctaac gggaaataca ccgcacagga tgcaacgaca gcacagaaag ggatagttca
     6301 gcttagcaac gcgaccaaca gcacatctga aatgctggcg gcaacgccaa agtcggtaaa
     6361 ggcagcctat gaccttgcta acgggaaata tactgctcag gacgctacga cagcacaaaa
     6421 aggaattgtc cagctcagta gtgcaaccaa cagcgcatct gaaacgcttg ccgcgacacc
     6481 gaaagcagtg aaagcagcta atgataatgc gaatggtcgg gtaccttctg cccgtaaggt
     6541 gaatggtaag gcgctttcat cggatataac actgacgccg aaagatattg gtacgcttaa
     6601 ctcaacaaca atgtcattca gcggtggtgc tggttggttc aaattagcaa cggtaaccat
     6661 gccacaggcg agttctgttg tttcaattac gttgattggt ggcgcgggat ttaacgtggg
     6721 gtcacctcaa caggcaggta tatctgaact tgttttgcgt gcaggtaatg gtaatccgaa
     6781 ggggattact ggtgctttat ggcagcgcac atcgacaggg tttacaaatt ttgcctgggt
     6841 caatacatct ggtgatactt acgatattta cgttgcaatc ggaaattatg cgactggtgt
     6901 aaatattcaa tgggattata ccagtaatgc cagcgtgacg attcatacgt caccagcata
     6961 ttctgctaat aagccggaag ggttaacgga cggtacagtt tattcactct atacgccatc
     7021 agagcagttt tatccgcctg gcgcaccaat cccgtggcca tcagataccg ttccgtctgg
     7081 ctatgccctg atgcaggggc agacttttga caaatctgca tacccgaaac ttgcagccgc
     7141 ttatccgtca ggcgtgatcc ctgatatgcg tggctggacg attaagggca aacctgccag
     7201 tggtcgggcc gtattgtctc aggaacagga cggcattaaa tcgcacaccc acagcgccag
     7261 cgcatccagt acggatttgg ggacgaaaac cacatcgtcg tttgattacg gcactaaatc
     7321 cacgaataac accggggcgc acacgcacag tgtgagcggt acagccgcaa gtgccggaaa
     7381 ccatactcat agtgtcacag gcgcatcagc agtcagccag tggtcacaaa atgggtcagt
     7441 acataaggta gtgtctgcgg ccagtgtgaa tacaagtgct gcaggagcgc acactcatag
     7501 tgtcagcggc acagctgcat ctgcaggtgc tcacgcacat actgtcggta ttggtgctca
     7561 tacgcactct gttgcgattg gctcacatgg acacaccatc accgttaacg ctgcgggtaa
     7621 cgcggaaaac actgtcaaaa acatcgcatt taactacatt gtgaggcttg cataatggca
     7681 ttcagaatga gtgaacaacc acggaccata aaaatttata atctgctgac cggaactaat
     7741 gaatttattg gtgaaggtga cgcatatatt ccgcctcata caggtctgcc agcaaacagt
     7801 acctatattg caccgccaga tattccggct ggcttcgtgg ctgttttcaa cagtgatgag
     7861 tcatcgtggc atctcgttga agatcatcgg ggtaaaacgg tttatgacgt ggcttccggc
     7921 gacgcgttat ttatttctga actcggctca ttaccggaaa atgttacctg gttatcgccg
     7981 gaaggggagt ttcagaagtg gaacggcaca gcctgggtga aagatgcaga agcagaaaaa
     8041 ctgttccgga tccgggaggc ggaagaaaca aaaaacagcc tgatgcaggt agccagtgag
     8101 catattgcgc cacttcagga tgctgtagat ctggaaatcg caacggagga agaaacctca
     8161 ttgctggaag cctggaaaaa gtatcgggtg ttgctgaacc gtgttgatac atcaactgca
     8221 cctgatattg agtggcctgt aatacctgaa gtttaaaata aaagcccgct taaaatatcg
     8281 cgggcttcga aatataaatg tattttcaga ggctagagct taggatatct cagcggcaat
     8341 taacttatga gggatagcta aataccagaa gataattagt ttttactatt tatctttgtt
     8401 tgtggttctc cttcagcaag ctcagcgcca gtgacaggat tgatgtcttc atgggatttc
     8461 aaccttgttg ccatagcttt tatcatagct tcttgcttgg tcgaaagatt tatcgaggcc
     8521 ttcccatccc caccgtcaac cgctgaaact ggatcggaaa catagtcaaa gttttcatca
     8581 ctgttccaac tacctctgac gtcttcacct tctgacatgt tgtaatatac gtttttgtat
     8641 ttctcaagtg gtggtaattt tcctggtgga aaggtgttac gaattgaatg taatgctttt
     8701 tcaaatgaaa gcatgtgtgc tgcttcccgg gtcattaaaa atgccagagt gtcttttact
     8761 ccaggatcat cagtaacatt aatgagacgt tcgtaaatga tctttgcccg agcttcagct
     8821 gcaatatttg aacgaagatc ggccgtgact tcgccaatag tatcaacata agctgcagtc
     8881 cagggtactc cagctgaatt agttaatgct gggcctcctc cgtagaggag agaagttata
     8941 tggctatcat tgccattttg agttaaggaa cggtaaagct cagcttcatt ctcagttcct
     9001 tcagcaagtg ctccttttgc acctttgttg agcataccaa caagagaacc aataatttcg
     9061 agatgactta gttcttctgt tgctatgtcc atcagcatat cccttcggcc tgcatcttca
     9121 tcacttaagc cttgagtgaa gtatcggcat gctgctgcaa gctcaccctg tggcccaccg
     9181 aattgttcta agagtagatt agccaagcct gggtttggct cacttacacg tactgtatat
     9241 tgaagttttt tcacgtgtct aaacataatg cctcctcaaa agaattattt tttggcttca
     9301 actccatcaa tttcagatct gaccatgaat tgctcagtgg tgtcaggaat atggttaaga
     9361 agccattcag ccatttgttt ttcttcatta agaattgctt caataatggg tactgaagct
     9421 gtatcacccg catttttggc tgctgcgaga agagatgtgt aacacgcaat ttcaaattgt
     9481 tcgaacacat agccactgat cgaacctttg acaatttcat cagatggaaa aattccacca
     9541 atggattgtc caaacgcggc cattttactc atagagtctt taatgacaga gcgcgaaata
     9601 ttattacgat caagtatgct ttcaagctgt gaaagctggt tctttgtttc gctaatgtgc
     9661 tgctcaattc tggaacgtaa ctcaggatag ttttctatcc ggctggccat agactcaagc
     9721 attttttcag cttgtttttc catcgcatgt gcatcacgaa gccagtcatg gtaatgttca
     9781 attctattca taatctgttt atcctaaaag aaattgaagt ctatagttca ggagttatcc
     9841 tgagcttttt tattaacgtt attaaatgcc aggtctgtta atttgacatc agtagctttt
     9901 tcctcttcca gtgtttcttt caggagttta gccgcttttt tatatcctaa ttgctcagcg
     9961 agtgttacca aagtgccata actggcaatt tcatagtgct ctactttttg tgctgcagca
    10021 atcaatgcag catcacgtac ttcattttta tcagtgcttt caattacctc attcgcctct
    10081 tcgataagtc cttccattgc ggcacactta attcttttca atttaagacc atcttcagaa
    10141 tcaacaacct gatcgatgcg ttcaatttga ccatgtgtct catcaagatg tgactgaaaa
    10201 gcagcggtta atttatcact atatgcagag cgactaagtt tagacagtgc ctt
//
LOCUS       DBFIFM010000164        10238 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.164, whole genome shotgun sequence.
ACCESSION   DBFIFM010000164 DBFIFM010000000
VERSION     DBFIFM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.164"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..921
                     /locus_tag="N7C74_003621"
     CDS             <1..921
                     /locus_tag="N7C74_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2433418.1"
                     /translation="KIEPSILPSLAWFALIVRAGSFSRAASEMGITLAALSQNLKSLE
                     ERLNTKLIYRTTRNISLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRINSS
                     RVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTAVP
                     VSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSKVVGGNEE
                     FEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFPGF
                     YIYVSSRVRMPSKVRAFIDFMVEKRVKLEN"
     gene            complement(1209..1565)
                     /locus_tag="N7C74_003622"
     CDS             complement(1209..1565)
                     /locus_tag="N7C74_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433419.1"
                     /translation="MNKNLILAFALFSLPVFAEEDLGPGKYVCDIRISSLDTATQILS
                     KSATVLDNGNNFIVQMPNGDQLYSPDLENVDDGIKQKATIGGVTFIRRPTFNDRFIVE
                     DGNTGFFYKMRNCEKK"
     gene            complement(1773..2333)
                     /locus_tag="N7C74_003623"
                     /pseudo
     CDS             complement(1773..2333)
                     /locus_tag="N7C74_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            2899..7152
                     /gene="fdeC"
                     /locus_tag="N7C74_003624"
     CDS             2899..7152
                     /gene="fdeC"
                     /locus_tag="N7C74_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HGU2433420.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLAKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNTSKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSAAVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTVKDAYDNPVTSLTPEAPSLAGAAAEGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQNAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLALSASLTGTASEGATVSSWTEKGNGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDTYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKADIELMSTVAGEHNISASVNGAQKTVTVKF
                     NADASTGQANLQVDAAAQKVANGKDAFTLTANVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADKATATVSGIE
                     VIGNYALADGNAKQTYKVTVTDANNNLLKDSEVTLTASPANLVLTPNGTAKTNEQGQA
                     IFTATTTVAAKYTLTAKVSQADGQESTKTAESKFVADDKNAVLTASSDVTSLVADGIS
                     TAKLEVTLMSANNPVGGNMWVDIKTPEGVTEKDYQFLPSKNDHFVSGKITRTFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKGEEAMN"
     gene            complement(7273..8130)
                     /locus_tag="N7C74_003625"
     CDS             complement(7273..8130)
                     /locus_tag="N7C74_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2433421.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIAISLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            8379..9248
                     /locus_tag="N7C74_003626"
     CDS             8379..9248
                     /locus_tag="N7C74_003626"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HGU2433422.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(9408..10001)
                     /gene="rclC"
                     /locus_tag="N7C74_003627"
     CDS             complement(9408..10001)
                     /gene="rclC"
                     /locus_tag="N7C74_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HGU2433423.1"
                     /translation="MEKYLHLLSRGDKIGLALIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQGSNESSSTLKTEY"
     gene            complement(10013..>10238)
                     /gene="rclB"
                     /locus_tag="N7C74_003628"
     CDS             complement(10013..>10238)
                     /gene="rclB"
                     /locus_tag="N7C74_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HGU2433424.1"
                     /translation="SVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLEDAIA
                     DIARKYNASSWKVTSMRIDNNSTATAVLYK"
BASE COUNT         2805 a         2207 c         2562 g         2664 t
ORIGIN      
        1 aaaatagagc cttcaatttt gccttctctt gcctggtttg cgctgattgt tcgtgccggc
       61 agtttttcca gagccgcttc tgaaatgggt attacgctgg ccgcattgtc ccagaattta
      121 aaatctcttg aggaacgtct taacaccaag ctgatatacc gtacgacccg caatatctcg
      181 cttactgagg aaggacagcg tctgtatgaa gtgctggtgt cggctttggg gcaaattgat
      241 gatgctttga aagatgttgg tgatacccaa cttgaaccta caggcctgtt gagaataaat
      301 tcctcccgtg ttgcagcacg gatgcttgtt gagcctcata ttggcgagtt tcttactcgt
      361 taccccaaaa caaagataga actcattatg gatgacggtt tatctaacat tattgcggac
      421 ggctgtgatg tgggcatacg tcttgagcaa ggactggatg agcatatgac tgccgttcct
      481 gtttctccgc ttattaaact ggttactgtg gcatcgccag attatttaaa agaacacggt
      541 attcctgaaa cgcctcagga actgagtaac cataattgcc ttcggttgcg gcataaaagt
      601 agcggtgcac tctctgcatg ggagttttct aaggttgtgg ggggtaatga agagttcgaa
      661 atagaggttt caggtaagta tatttctaat gatgacgaaa gcatgatacg aatggcgctg
      721 aatggaacgg gtatcattca acatctggat tttgcaatcg ctgaacatat caatgcgggc
      781 aagttacagc cgattcttga ggattgggct gtttcatttc ctgggtttta tatttatgtt
      841 tcatcacggg tgagaatgcc ctctaaagtt cgtgctttta ttgattttat ggtggagaaa
      901 agagtaaaac tcgaaaattg atgatatgtg taattgatgg ttgctctggg cgaatatcgg
      961 taagaggaat aacgtaatca ttaggatgcg tttacataat aaagtttctg ataattaata
     1021 cttattttct atacggccat aatatcaatt atcattatca ccagtaatgt ggttaaaata
     1081 atagtatcta tgaccgctac ggttaacggt ggttattttg ggaggcgtta acacgtagcg
     1141 gcttgtagtt gtaagtcttg aacaggtaca aagcctgaaa tcaggaggat atcgctatca
     1201 aacttccttt atttcttctc gcagtttcgc atcttataga agaatcctgt atttccatct
     1261 tccacgatga agcgatcgtt aaaagttgga cgacgaataa atgtcactcc acctattgtt
     1321 gctttttgtt ttataccatc atcaacattt tccagatctg gagagtacaa ttgatctcca
     1381 tttggcattt gaacgatgaa attgtttcca ttatcgagaa ctgttgcgga ttttgacaga
     1441 atttgagtag cggtatccag gctggaaatc ctaatgtcac aaacatattt gcctggcccc
     1501 agatcttctt ctgcaaatac aggtaaggaa aataatgcaa atgcgaggat gagattctta
     1561 ttcatgcaaa ctccaggaag atcaataaaa gcaatatgtt acataaaaag cacgtaaatg
     1621 aaaagagcga ttggttaatg agtaatgcta ttaatccccg catggcctac ttttcattgg
     1681 ctgcggtgtt gaacctcatt gaatatgggc aagactctga ctgagtgacc gacgtaaata
     1741 ggtagatgca taatcgctta ccgccctgta actgcaaacg gtgcccaggc ttctggctga
     1801 ataccacggc tttgcatcca cggaacggcg tgtagctgct gattgaaagg gttaacttca
     1861 acctggttta ccgctggaac gacgttatta aaggcgataa ggtcagccag tcggtcagga
     1921 tggaagttac tgacgccaat agcgcgaatt ttaccggcct gttgcagctc ttccatggca
     1981 cgccaggcac cgtggacatc accgtaaggc tgatgaatca aatacaggtc aacgtaatca
     2041 agttgcagtc gattcagaga gcgttcgaac tgggctttgg ccccttcgta actggtatcc
     2101 tgcaaccaga gtttcgtggt gacaaagagt tcgttacggg cgataccgct ttgtttcagt
     2161 gcgttcccga cctggggttc attttgatag gaggcggcgg tgtcaatcag tcgataaccg
     2221 gtgttaatag cgtcaatgac cgctctttcg cattcggcgg catcggtcat ctgaaataca
     2281 ccaaagccca gcaggggcat ttcaataccg ttattcagtt ttactttttg catggtgtta
     2341 tccttccgtt atcgttcatg agaagcataa cgtaaagaga agagctcgat tagacggcag
     2401 gatttgtcag gggttatgaa cgaaattcat aaatctgttt gagtgttgcg atgggtagtg
     2461 caagttcgat atctccgcaa tttacagtcc gatgaaggaa aatgaatatc cataaaaaat
     2521 atatcggttt atcctggcgt atatacctac ttcgacgtat ttccaatagt tgctaattaa
     2581 aggcaggtca ttattattca ctctgaataa tgaattattc actgtccgca gagtaagaaa
     2641 tataacttaa gtatctattt aatgacttgc acaaaaagct aaattttcac ccataaataa
     2701 aaatataatc ccgcgcccaa ccacctgatg agtggctaca ggcactggat atattaggtg
     2761 gcggtgtact ttcttacata aaggtatttc cttttctgcg gaaaaggaaa ccgggaaatc
     2821 cccggttttt ctgacaagca gacgccatta tttgtgtctg cctatgttcg ttaattcgtt
     2881 catcaggaaa ttatctcaat gtcacgttat aaaacaggtc ataaacaacc acgatttcgt
     2941 tattcagttc tggcccgctg cgtggcgtgg gcaaatatct ctgttcaggt tctttttcca
     3001 ctcgctgtca cctttacccc agtaatggcg gcacgtgcgc agcatgcggt tcagccacgg
     3061 ttgagcatgg gaaatactac ggtaactgct gataataacg tggagaaaaa tgtcgcgtcg
     3121 tttgccgcaa atgccgggac atttttaagc agtcagccag atagcgatgc gacacgtaat
     3181 tttattaccg gaatggccac cgctaaagct aaccaggaaa tacaggagtg gctcgggaaa
     3241 tatggtacag cgcgcgtcaa actgaatgtc gataaagatt tctcgctgaa ggattcttcg
     3301 ctggaaatgc tttatccgat ttatgatacg ccgacaaata tgttgttcac tcagggggca
     3361 atacatcgta cagacgatcg tactcagtca aatattggtt ttggctggcg tcatttttca
     3421 ggaaatgact ggatggcggg ggtgaacacc tttatcgacc atgatttatc ccgtagtcat
     3481 acccgcattg gtgttggtgc ggaatactgg cgcgattatc tgaaactgag cgccaatggt
     3541 tatattcggg cttctggctg gaaaaaatcg ccggatattg aggattatca ggaacgcccg
     3601 gcgaatggtt gggatatccg cgcagagggc tatttacctg cctggccgca gcttggcgca
     3661 agcctgatgt atgaacagta ttatggcgat gaagtcgggc tgtttggtaa agataagcgc
     3721 cagaaagacc cgcatgctat ttctgccgag gtgacctata cgccagtgcc tcttctgaca
     3781 ctgagcgccg ggcataagca gggcaagagc ggtgagaatg acactcgctt tggcctggaa
     3841 gttaactacc gaattggcga acctttggcg aaacaactcg atacggatag cattcgcgag
     3901 cgtcgggtac tggcaggcag ccgctatgac ctggttgagc gtaataacaa catcgttctt
     3961 gagtaccgca aatctgaagt gatccgtatt gctctgcctg agcgtattga aggtaagggc
     4021 ggtcagacac tttccctggg gcttgtggtc agcaaagcaa ctcacggact gaaaaatgtg
     4081 cagtgggaag cgccgtcatt actggctgaa ggtggcaaaa ttaccggtca gggtagtcag
     4141 tggcaagtaa cgctcccggc ttatcgtcca ggcaaagaca attattatgc gatttcagca
     4201 gttgcctacg ataacaaagg caatacctca aaacgcgtgc agacagaggt ggtcattacc
     4261 ggagctggta tgagcgccga tcgcacggcg ttaacgcttg acggtcagag ccgtattcaa
     4321 atgcttgcta acggtaatga gcaaaaaccg ctggtgctgt ctctgcgcga cgccgagggc
     4381 cagccagtca cgggcatgaa agatcagatc aagactgaac taactttcaa accggctgga
     4441 aatattgtga ctcgttccct gaaggccact aaatcacagg caaagccaac actgggtgag
     4501 ttcaccgaaa ctgaagcagg ggtgtatcag tctgtcttta ctaccggaac gcagtcaggt
     4561 gaggcaacga ttactgttag cgttgatggc atgagcaaaa ccgtcactgc agaactgcgg
     4621 gccacgatga tggatgtggc aaactccacc ctgagcgcta acgagccgtc aggtgacgtg
     4681 gttgctgatg gtcagcaagc ctatacgttg acgttgactg cggtggactc cgagggtaat
     4741 ccggtgacgg gagaagccag ccgcttgcga tttgttccgc aagacactaa tggtgtaacc
     4801 gttggtgcca tttcggaaat aaaaccaggc gtttacagcg ccgcggtttc ttcgacccgt
     4861 gccggaaacg ttgttgtgcg tgctttcagc gagcagtatc agctgggcac attacaacaa
     4921 acgctgaagt ttgttgccgg gccgcttgat gcagcacatt cgtccatcac cctgaatcct
     4981 gataaaccgg tggttggggg gacagttacg gcaatctgga cggtaaaaga tgcctatgac
     5041 aaccctgtga ccagcctcac gccggaagcg ccgtcattag cgggtgccgc tgctgaaggt
     5101 tctacggcat cgggctggac aaataatggt gatgggacgt ggactgcgca gattactctc
     5161 ggctctacgg cgggtgaatt agaagttatg ccgaagctaa atggacagaa tgcggcagca
     5221 aatgcggcaa aagtaaccgt ggtggctgat gcgttatctt caaaccagtc gaaagtctct
     5281 gtcgcagaag atcacgtaaa agccggcgaa agcacaaccg tgacgctggt ggcgaaagat
     5341 gcgcatggca acgctatcag tggtcttgcg ttgtcggcaa gtttgacggg gaccgcctct
     5401 gaaggggcga ccgtttccag ttggaccgaa aaaggtaacg gttcctatgt tgctacgttg
     5461 actacaggtg gaaagacggg cgagcttcgc gtcatgcctc tcttcaacgg ccagccagca
     5521 gccaccgaag ccgcgcagtt gacggtcatc gccggagaga tgtcatcagc gaactctacg
     5581 cttgttgcgg acaataaggc tccgaccgtc aaaacgacga cggaactcac cttcaccgtg
     5641 aaggatgcgt acgggaaccc ggtcaccggg ctgaagccag atgcaccagt gtttagcggt
     5701 gccgccagca cggggagtga gcgtccttca gcaggaaact ggacagagaa aggtaatggg
     5761 gtctacgtgt cgaccttaac gctgggatct gccgcgggtc agttgtctgt gatgccgcga
     5821 gtgaacggcc aaaatgccgt tgctcagcca ctggtgctga acgttgcagg tgacgcatct
     5881 aaggctgaga ttcgtgatat gacagtgaag gttaataacc aactggctaa tggacagtct
     5941 gctaaccaga taaccctgac cgttgtggac acctatggta acccgttgca ggggcaggaa
     6001 gttacgctga ctttaccgca gggtgtgacc agcaagacgg ggaatacagt aacaactaat
     6061 gcggcaggta aagcggacat tgagcttatg tcaacggttg cgggagaaca caatatttcc
     6121 gcttcggtga atggtgctca gaagacggtc acggtgaaat tcaacgcgga tgccagcacc
     6181 ggtcaggcaa acctgcaggt agacgccgct gctcaaaaag tggcaaacgg caaagatgcc
     6241 tttacgctga cggcgaacgt tgaggataaa aatggtaacc ctgttccagg gagcctggtg
     6301 acctttaatc tgccccgggg tgtcaagccg cttacaggcg ataatgtctg ggtgaaagcc
     6361 aacgatgagg ggaaagcaga gttgcaggtg gtttcagtga ctgccggaac gtatgagatc
     6421 acggcatcgg cagggaatag ccagccttcg aatacgcaga ctataacgtt tgtagccgat
     6481 aaggctaccg caaccgtctc cggtattgag gtgattggca actatgcact ggcggacggc
     6541 aatgccaaac agacgtataa agttacggtg actgatgcca ataacaacct gttgaaagat
     6601 agcgaagtga cgctgactgc cagcccggca aatttagttc tgactcccaa tgggacggcg
     6661 aaaactaatg agcaaggaca ggctattttc accgccacga ccactgtcgc agcgaaatat
     6721 acactcacgg cgaaagtgag tcaggccgac ggtcaggaat cgacgaaaac tgccgaatct
     6781 aaattcgtcg cggatgataa aaatgcagta ctcaccgcat catctgatgt gacttctctg
     6841 gtggcggatg ggatatcgac tgcgaagctg gaggtgacac tgatgtcggc aaataacccc
     6901 gttgggggga atatgtgggt cgacattaag acgccagaag gggtgacgga gaaggattat
     6961 cagttcctgc cgtcgaaaaa tgaccatttc gtgagcggaa aaatcacgcg tacatttagt
     7021 accagcaagc ctggtgtcta tacgttcaca tttaacgccc tgacgtatgg cgggtacgaa
     7081 atgaagccag tgacggtgac cattaccgcg gtggatgccg atacggcaaa gggcgaggag
     7141 gcgatgaact aatattaaga atatggaagt aacattctaa taaacaataa tggcgttgac
     7201 atcttcaacg ccattattta ttgaattgcc tgacagtagt ctcgttataa gttcatttag
     7261 atatagcgac agctaaaccg gacgaacagg gataaatatt tcgcaaacaa catcattgct
     7321 atccttttct acgaaatctt cattgtaata aaaccgctca atatcactgc cgttcactct
     7381 ggctaacccc ctggcgggta attccatcag atagaggttt cgcgcccatt cagggtagtg
     7441 ctcccactca ccttcatagc ggaaagaggc atacagtcct cggttaattg tccactcttt
     7501 cggcatctct gaatcggttt gtttgcatac cgtaccggca aaagtttcaa ctgtgagtaa
     7561 atcgaccgat tttgttgacg gttcaaacgt ggagacgaaa tagatatccg tcttaaaatt
     7621 cttcatacat ctggctaaat gatgacgtcg cagtttgact tcttcatccg gtgatttatt
     7681 cgacacttca atatcatatt taaatgaatt gccaaaaata ggtgtctcag gaaagttgac
     7741 taatctacac tccgtctttt gctgttgacg tattaaaaaa gaagggaaga tatttgcgag
     7801 atcccaataa tcacggtggc ggtatacacg gggagagcag ccgaataact ttttaaattc
     7861 gcggctgaat gactgctgtg aatcaaaatg caaagagatt gcaatatcaa gcatagattt
     7921 cgcggtgagc cggacaagaa tggcggcacg acaaagcctt cgtttgcgaa tgtattctcc
     7981 caaaggcaca tgcatgaaat ttcggaacag aagctggatg ttgcgtctgc tgtagccaga
     8041 tttctgtgcg atatcttcga ttgaaatagg gtgctcaaga ttgcactcaa tccactccag
     8101 gagctgctgt agaatcttct gcctgatcat cacattctcc gttaattctt tgtcatatcg
     8161 cacattttcg ttatgccctg ggccctaagt gtatcaaaaa cagcaagggc gaaggcaaaa
     8221 gcactggtag cgtctgcttc gttgtccccg aaaatagaga ggtttcagtc ctacattatt
     8281 aatgaatttt ttgcataagt gatatcaaaa tccacatact aatttgaggt tacgttttaa
     8341 cgtagactca ttgttcatgc ctaatggagg gactgacagt ggaattttcc gtattaagta
     8401 acaatctgaa aatgccgatg atgggatttg gtgtttttca ggttaccgat aaaaacgtgt
     8461 gcaaacagtc agtgttgaac gctatccgca cggggtatcg actcatcgac actgcagcgg
     8521 tatacggtaa tgaagatgcc gtcggtgaag ccgttcgtga agctatatcc gaaggtttat
     8581 gtacccgaga agagttattt ataacgtcta aattgtgggt gcaggatatg ttgaatcagg
     8641 atacggcagc agcaggtatt gaagcatcat taaaaaaatc gggactagaa tacttcgacc
     8701 tctatttatt gcatcaggct atgcgcgatt atttcagtgc gtggcgtgca ctcgaagatg
     8761 cctatgaaga gggcaaatta aaagcgattg gggtttccaa tttctatcct catgttctgg
     8821 cgaacttttg tgaaacggta agagttaaac cgatggtcaa ccaggtcgag ttgcatccct
     8881 attttgccca accagaggcg ctggcaacca tgaagtatta taacgtgcag cctgaagcat
     8941 gggctccgtt aggtggtgga cgacataaac cctttgaaaa taatctgctt cagagtattg
     9001 cagatgccca tcaaaaatcg atttctcaag tcattctgcg ttggaatatt caacggggag
     9061 tggtcgttat tccgaaatcg acacatcaac agcgtatcga agaaaatttt gctatctggg
     9121 atttctcact gacagagaaa gaaatggcac aaattagttc gcttgatttg ggttatgttg
     9181 gggagtcggt aaaacatttt aatcctgaat ttgttcgtgg ttgtcttgct gtaaaaatac
     9241 atgattgata ttaatcatgt attttacctg agacgacaag aatcttttaa caggggagtg
     9301 atattgatct tcactctgtc atatctccgg taatatggcg tcaggcttca tcatcagaaa
     9361 gggggcctga cgcctgaaaa agtgaacaac agacagtgtt cggattatca atattcagtt
     9421 tttaacgttg aactggattc attactgcct tgtttaagaa tttcccgcgc cgaatctgcc
     9481 attatcatga cagcacctgc cagcatcaga gtatctttca ataccagacg accagcaccg
     9541 gataaataag ggaaaccatg atgagcgtca cccaatgcgg gtacccatgc ctccggggtg
     9601 gtgattaaaa atgagagtgt taccagcggt gtggtaaatg ccatcagccc tcccaataaa
     9661 cctaaccagc gattgacagg attagccaaa accagcagag caataatcac ctccacgacg
     9721 ccaagaccgt tggaaaaacc ataggtatta ttggccgatt gccatgccct tgcttctggt
     9781 ttgtattcgc cttcgtgagt cagatactgt ttatagtctt ccgggtgttc ataaaagaac
     9841 gacattagtg gactgtttgc gacgaatggt gtaatgctgt ctgcctcgta agggacaaac
     9901 tttaataacc caatccacat aaaaacaatc gcaatactca gacgaatcaa tgccaggcca
     9961 attttatctc cccgacttaa caggtgcagg tatttttcca ttgtgaatat ccttatttat
    10021 acaatactgc tgttgcggtt gaattattat caattcgcat cgatgtgact ttccaggatg
    10081 aagcattata ttttctggct atatcagcaa tggcatcctc cagaactgtc ggtgatgctg
    10141 atactgacga gacgctgata tgtttcagaa ttattttatc atctgcattg gtggaaaatg
    10201 ccataacgcc agataatagt gttgcaaata aaacggat
//
LOCUS       DBFIFM010000165        10200 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.165, whole genome shotgun sequence.
ACCESSION   DBFIFM010000165 DBFIFM010000000
VERSION     DBFIFM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.165"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            65..640
                     /gene="ssuE"
                     /locus_tag="N7C74_003629"
     CDS             65..640
                     /gene="ssuE"
                     /locus_tag="N7C74_003629"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HGU2433425.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAA
                     EDLLYARFDSPALHTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLHSLRGNAHA"
     gene            633..1592
                     /gene="ssuA"
                     /locus_tag="N7C74_003630"
     CDS             633..1592
                     /gene="ssuA"
                     /locus_tag="N7C74_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HGU2433426.1"
                     /translation="MRNIIKLALVGLLSVSTIVVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLLYVG
                     VEPPKPKAEVILVAENSPIKTAADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1589..2734
                     /gene="ssuD"
                     /locus_tag="N7C74_003631"
     CDS             1589..2734
                     /gene="ssuD"
                     /locus_tag="N7C74_003631"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HGU2433427.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVENDFIPRRVAQS"
     gene            2746..3537
                     /gene="ssuC"
                     /locus_tag="N7C74_003632"
     CDS             2746..3537
                     /gene="ssuC"
                     /locus_tag="N7C74_003632"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HGU2433428.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVTVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3534..4301
                     /gene="ssuB"
                     /locus_tag="N7C74_003633"
     CDS             3534..4301
                     /gene="ssuB"
                     /locus_tag="N7C74_003633"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HGU2433429.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(4344..6956)
                     /gene="pepN"
                     /locus_tag="N7C74_003634"
     CDS             complement(4344..6956)
                     /gene="pepN"
                     /locus_tag="N7C74_003634"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HGU2433430.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLCLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7222..8424
                     /gene="pncB"
                     /locus_tag="N7C74_003635"
     CDS             7222..8424
                     /gene="pncB"
                     /locus_tag="N7C74_003635"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HGU2433431.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLEIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8593..9993
                     /gene="asnS"
                     /locus_tag="N7C74_003636"
     CDS             8593..9993
                     /gene="asnS"
                     /locus_tag="N7C74_003636"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HGU2433432.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQTSKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTEAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2283 a         2621 c         2771 g         2525 t
ORIGIN      
        1 gacagactga gcgaattgtt atcaatgtta acaaaacaag aacaattggt tataaggaga
       61 gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct ccagctcctt
      121 gctggaatat gcgcgggaaa aactaaatgg cctggatgtt gaggtttatc actggaatct
      181 gcaaaacttc gccgcggaag atctgcttta tgctcgtttc gatagtccgg cactccatac
      241 cttcaccgaa cagttgcaac aggctgacgg gctgattgtc gccacgcctg tgtataaagc
      301 cgcttattcc ggtgcgctga aaaccctgct cgacctgctg ccagaacgtg cgttgcaagg
      361 caaagtggtg ctaccgctgg cgacgggcgg tacggtggcc catctgctgg cggtcgatta
      421 tgcccttaag ccagttttaa gtgcgctgaa agcccaggag atcctgcacg gcgtgtttgc
      481 cgatgactca caagtaattg attaccatca caaaccccag ttcacgccga atttacaaac
      541 ccgtcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg atgttcaggt
      601 tcctgatctt cattctctgc gaggtaatgc ccatgcgtaa catcattaaa ctggcgctgg
      661 tgggattgct tagcgtctct acgattgtgg ttgctgcaga atcctcgcct gaagcgttac
      721 gtataggcta tcagaaaggc agtattggta tggtattggc aaaaagccac cagttactgg
      781 aaaaacgcta tccgcaatca aaaatctcgt gggtggagtt ccccgctggc ccgcaaatgc
      841 ttgaggcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt ccgccaatct
      901 tcgcccaggc tgccggggct gatttgctgt acgtgggcgt cgagccgccg aagccaaaag
      961 ccgaggtgat tctggtggca gaaaacagcc cgatcaaaac cgcagccgat cttaaaggcc
     1021 acaaagttgc gttccagaaa ggttccagtt cacacaatct tttactgcgc gcactacgcc
     1081 aggccggact taagtttact gacatccagc ccacttatct gacgccagct gatgcccgcg
     1141 ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac tactccgctg
     1201 cattattaca gggcggcgtg cgggtgttga aagacggcac cgatctcaat caaaccggat
     1261 cgttttatct ggcagctcgc ccctatgcag aaaaaaacgg cgcttttatt cagggtgtac
     1321 tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgagcaa agtatcgctt
     1381 tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta gatcatcgcc
     1441 ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag caaacggcag
     1501 atctgtttta tgaaaatcgt ctggtgccga aaaaagtcga tattcgccaa cgcatctggc
     1561 agcccactca actggaagga aaacaattat gagtctgaat atgttctggt ttttaccgac
     1621 ccatggtgac gggcattatc tgggaacgga agaaggttca cgcccggttg atcacggtta
     1681 tctgcaacaa attgcgcaag cggcggatcg tcttggctat accggtgtgc tgatcccaac
     1741 ggggcgctcc tgcgaagatg cgtggctggt tgccgcatcg atgatcccgg tgacgcagcg
     1801 gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca cctaccgttg ccgcccgcca
     1861 ggccgccacg cttgaccgtc tctcaaatgg acgtgcgttg tttaacctgg tcacaggcag
     1921 cgatccacaa gaactggcag gcgacggcgt gttccttgat catagtgagc gctacgaagc
     1981 ctcggcggaa tttacccagg tctggcggcg tttattgctg ggggaaaccg tcgatttcaa
     2041 cggtaaacat attcatgtgc ggggagccaa actgctcttc ccgccgattc aacagccgta
     2101 tcctccgctt tactttggcg gatcgtcaga cgtcgcccag gagctggcgg cagaacaggt
     2161 tgatctctac ctcacctggg gcgaaccgcc ggaactggtt aaagagaaaa tcgaacaagt
     2221 gcgggcgaaa gctgccgcgc atggacgcaa aattcgtttc ggcattcgtc tgcatgtgat
     2281 tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct cgcatcttga
     2341 tgatgagact atcgccaaag cacaagccgc attcgcccgc acggattccg tggggcaaca
     2401 gcgaatggca gcgctacata acggcaagcg cgacaatctg gagatcagcc ccaatttatg
     2461 ggcgggcgtt ggcttagtgc gcggcggtgc cgggacggcg ctggtgggcg atggtcctac
     2521 ggtcgctgcg cgaatcaacg aatatgccgc gcttggcatc gacagctttg tgctttcggg
     2581 ctatccgcat ctggaagaag cgtatcgggt cggcgagttg ctgttcccac atctggatgt
     2641 cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag cggtggagaa
     2701 tgattttatc cctcgcagag tcgcgcaaag ctaaggaaga agataatggc aacgccagtg
     2761 aagaagtggt tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt gacggtgtgg
     2821 caactggcct cctcggttgg ctggttgtcg acgcgtattt tgccctcacc ggaaggggtg
     2881 gtgatggcgt tctggacgct ctcagccagc ggcgaattgt ggcagcatct ggcgatcagc
     2941 tcctggcggg cgctgattgg tttttcaatt ggcggctcgc tggggttgat tttgggactg
     3001 attagcggac tgtcgcgctg gggagaaagg ctgctggata cctcaattca gatgttgcgc
     3061 aacgtgccgc atctggcgct gatcccgctg gtgattttgt ggtttggcat tgatgaatcc
     3121 gcaaaaatct ttctggtggc gctcggtacg ctattcccca tttatatcaa cacctggcat
     3181 ggcatccgta atatcgatcg cgggttggtg gagatggcgc gtagctatgg attatccggc
     3241 ataccgctgt ttatccatgt gatcctgcct ggtgccctgc cctcaattat ggtcggcgtg
     3301 cgttttgcgt tgggcctgat gtggctgacg ctgattgttg ccgaaaccat ttctgccaat
     3361 tcaggcattg gttatctggc gatgaatgcg cgggagtttt tgcaaacgga cgtggtggta
     3421 gtcgctatta ttctttacgc cctgctcggc aaacttgctg acgtcagcgc gcagctgtta
     3481 gagcgcctct ggttgcgctg gaacccggct tatcatttga aggaggccac ggtatgaata
     3541 ctgctcgtct gaaccagggc acaccattgt tgctcaatgc agtaagcaaa cattacgcgg
     3601 aaaatatcgt cctgaaccaa ctggatttac atattccggc aggtcagttt gtggcggtgg
     3661 tgggccgcag cggtggtggc aaaagtaccc tgctgcgcct gctggcaggt ctggaaacgc
     3721 caaccgctgg cgatgtgtta gcgggcacca caccgctggc tgaaattcag gaagacacgc
     3781 gaatgatgtt tcaggatgcg cgtctgctac cgtggaaatc ggtgattgat aacgttgggt
     3841 taggccttaa aggccagtgg cgcgatgccg cgcgtcgggc gttggctgcg gtaggactgg
     3901 agaatcgcgc cggggaatgg cctgccgcac tttctggcgg gcagaaacag cgagtggcgc
     3961 tggcaagagc gttgattcat cgaccgggtt tattgttgct tgatgaaccg ctcggtgcgc
     4021 tggatgcctt aacgcgactc gagatgcagg atttgattgt gtcactttgg caggagcacg
     4081 gttttaccgt actgttggtg acgcatgatg tgagcgaagc ggtagcaatg gctgatcggg
     4141 tgttgttaat tgaagaggga aaaattggtc tggatttgac ggtggatatt ccccgtccgc
     4201 gccggttagg gtcggtgcga ttagcggagc tggaagcgga agtgttgcaa cgggtgatgc
     4261 agcgtggtga atctgaaact cgcctgagaa aacaggggta aaatccccga atggcggcgc
     4321 tattgccgcc attcggggat ttattaagcc agtgctttgg tgatcttctc gtacagatcg
     4381 ccagatagat tttccagccc tttcaactgt tccagcgccg cgcgcatttt ctcctgacgt
     4441 ttggcatcat aacgtttcag gcgaatcagc ggttcaatca ggcgtgaagc tacctgcggg
     4501 ttacggctgt tcaggtcggt aagcatttcc accaggaact ggtaaccgct cccatcttcg
     4561 gcatggaacg ctgccgggtt gctgcccgca aacgcaccaa tcaacgaacg aatgcggttc
     4621 gggttgctca tggtaaatga gcgatgctgc aacaggccgc gcaccgtctc cagcacattc
     4681 gccgccgggc tggtggcttg caggataaac catttatcca tcaccagacc atcctgatgc
     4741 cacttgtcgt cgtactcctg catcagcgcg tcacggcaag gcagctgtgc agcaaccgcc
     4801 gcagaaagcg ctgccagcgc atcggtcata ttgtttgctt catggtattg cttgctcacc
     4861 agcacgtcag ccagatgcgt ttcaccaaaa gcaaggaagc gcaggcaggc attacgcaga
     4921 gtgcgttttg caatatcttc atgctcaaca cggtactcgc tctggtaatt cgcgttgtaa
     4981 atagccagta gctcatccgc cagttcagtc gccagagtac gagtgagtgc ttcgcgtact
     5041 tcggcaatag caatcgggtc gatgatatcg aacagttcag ccatttcatt gacagaaggc
     5101 agcgtcagga tttctgccgc cagcgccgga tcaatcttct catcgagcag caccgcgcgg
     5161 aaagcgtcag ccacatgtac cggcagagac agcggctgcc cttgctgatg acgggcgacg
     5221 ttcagcttga tgtaggttgc cagcaaactt tgcgccgcat cccaacggga aaaatcatta
     5281 cgcgcatgac gcatcaggaa ggtcagttgc tgatcgctcc acttatattc cagtttcact
     5341 ggcgcagaga attcgcacag cagcgcaggc accggctgga agtagacatt atcaaaaaca
     5401 aaggtctgtt ccgcctgggt gacgttcagc acggaattca ccggatgacc gcctttctgc
     5461 aacgggatca ctttgccttc gttgtcatac aattcgatgg caaacggaat atgcagcggc
     5521 tgtttttctg cttgatccgg cgtggctggc gtgcgctggc tgatggtcag ggtgtactgc
     5581 tcggtttccg gattgtagtc gtctttgacg gtcacaaccg gcgtaccgga ctggctgtac
     5641 caacggcgga aatgggagag atcgacattc gacgcatctt ccatcgcctg aacgaaatcg
     5701 tcgcaggtcg ctgcactgcc atcatggcgc tcgaaataga gctgcatccc tttctggaag
     5761 ttttcttcgc caagcagagt gtggatcata cgaatcactt ccgcgccctt ctcgtaaacg
     5821 gtcagggtgt agaaattgtt catctcaatg accatgtccg ggcggattgg gtgcgccatc
     5881 gggctggcgt cttccgcaaa ctgcaatccg cgcatggtgc gcacattatt gatacggttt
     5941 accgcgcggg aaccaaggtc agagctgaat tcctgatcgc ggaagacggt taaaccttct
     6001 ttcaggctga gctggaacca gtcgcggcag gtcactcggt taccggtcca gttatggaaa
     6061 tattcatggc cgataacgcg ttcaatatcg aggtaatctt tgtcggtggc ggtatcggtg
     6121 cgggccagca catatttgga gttaaagata ttcagaccct tattctccat tgcgcccata
     6181 ttgaagaaat ccaccgcgac gatcatatag atgtcgaggt catactccag gccaaagcgt
     6241 tcttcatccc atttcatgga gtttttcagc gaggtcatcg cccacggcgc gcgatcaagg
     6301 ttgccgcgat cgacgtacag ctccagtgct acttcgcgac cagaacgcgt ggtaaaggta
     6361 tcgcgcagta catcaaagtc gcctgccacc agcgcaaaca ggtagcacgg tttcgggaac
     6421 gggtcctgcc actgtaccca atggcgtccg ttttccagtt cgccttgcgc aacgcggtta
     6481 ccgttggaaa gcaggaaggg atatttggtt ttatcggcaa taattttggt ggtaaaacgc
     6541 gccagcacgt ccgggcggtc gagataatac gtaatatggc ggaaaccttc ggcttcacac
     6601 tgggtgcaaa gcgcatcgcc tgactgataa agcccttcca gcgccgtatt cgccgccggg
     6661 cttatttcat taatgatctt aagcgtaaaa cgctccggca aattactgat gaccagtgcg
     6721 ccctcttctt ctttccaggc ggtccacggc tcatcattaa tatgaacaga aaccagtttg
     6781 aggtcttcgc cgttgagaca aagcggagca tctgatgcac catgacggac agcctggctg
     6841 accgcggtaa cgaccgtctt ttgcgcgtcg aggtcaaagg tcaagtcaat atcagtaatc
     6901 tggtaatccg gcgcacgata atcgtgacgg tatttggctt gtggctgttg agtcataaat
     6961 aacctttagc atcttttata gagtctggtg ttcagtctat tcctgttgcg taaatcgcgc
     7021 tatgcagaat cttcatcttt tcaggtacaa acgcctttat tgctacattt ttataacata
     7081 caccgcgtaa tgccatcgac cagaaaggtg gcatatggtg tgatcggggt tcaataaatt
     7141 gcgaaacaag gtatactcca gcagttcctg aagatgttta ttgtactaaa cgctcctgta
     7201 cgaggacgct actgcgcacc tatgacacaa ttcgcttctc ctgttctgca ctcgttgctg
     7261 gatacagatg cttataagtt gcatatgcag caagccgtgt ttcatcatta ttacgatgtg
     7321 catgtcgcgg cggagtttcg ttgccgaggt gacgatctgc tgggtattta tgccgatgct
     7381 attcgtgaac aggttcaggc gatgcagcac ctgcgcctgc aggatgatga atatcagtgg
     7441 ctttctgccc tgcctttctt taaggccgac tatcttaact ggttacgcga gttccgcttt
     7501 aacccggaac aagtcaccgt gtccaacgat aatggcaagc tggatattcg tttaagcggc
     7561 ccgtggcgtg aagtcatcct ctgggaggtt cctttgctgg cggttatcag tgaaatggta
     7621 catcgctatc gctcaccgca ggccgacgtt gcgcaagccc tcgacacgct ggaaagcaaa
     7681 ttagtcgact tctcggcgtt aaccgctggt cttgatatgt cgcgcttcca tctgatggat
     7741 tttggcaccc gtcgccgttt ttctcgcgaa gtacaagaaa ccatcgttaa gcgtctgcaa
     7801 caggaatcct ggttcgtggg caccagcaac tacgatctgg cgcgtcggct ttccctcacg
     7861 ccgatgggaa cacaggcaca cgaatggttc caggcacatc agcaaatcag cccggatcta
     7921 gccaacagcc agcgagctgc acttgctgcc tggctggaag agtatcccga ccaacttggc
     7981 attgcattaa ccgactgcat cactatggat gctttcctgc gtgatttcgg tgtcgagttc
     8041 gctagtcggt atcagggcct gcgtcatgac tctggcgacc cggttgaatg gggtgaaaaa
     8101 gccattgcac attatgaaaa gctggaaatt gatccacaga gtaaaacgct ggttttctct
     8161 gacaatctgg atttacgcaa agcggttgag ctataccgcc acttctcttc ccgcgtgcaa
     8221 ttaagttttg gtattgggac tcgcctgacc tgcgatatcc cccaggtaaa acccctgaat
     8281 attgtgatta agttggtaga gtgtaacggt aaaccggtgg cgaaactttc tgacagccct
     8341 ggcaaaacta tctgccatga taaagcgttt gttcgggcgc tgcgcaaagc gttcgacctt
     8401 ccgcatatta aaaaagccag ttaatatcat cagggagcta atcggctccc tttttttacc
     8461 tttaattccg aaatctttcg ctgcatttgc gaattctgct tgtctgattg cagatgccag
     8521 gtaacatagg tatcccccca tttacgggtg gacaagtgtt tattttttcc gactattaac
     8581 agagagaata ttatgagcgt tgtgcctgta gccgacgtac tccagggccg cgtagccgtt
     8641 gacagcgaag tcaccgtacg cggatgggta cgtacccgcc gagattcaaa agctggcatc
     8701 tccttcctcg ccgtttatga cggttcctgc tttgatcctg tacaggctgt catcaataat
     8761 tctctgccca attacaatga agacgtcctg cgtctgacca ccggctgctc ggtcattgtg
     8821 acgggtaaag tcgtggcgtc gccgggccag gggcaacaat ttgaaattca gaccagcaag
     8881 gttgaagttg ctggttgggt tgaagatcca gacacttacc cgatggcggc aaaacgccac
     8941 agcattgagt atctgcgtga agtcgctcac ctgcgtccgc gcacaaacct gattggtgcc
     9001 gtcgcgcgcg ttcgccatac gctggcgcag gcgctgcatc gcttctttaa cgagcaggga
     9061 ttcttctggg tttcaacgcc actgattact gcgtctgata ccgaaggggc tggcgaaatg
     9121 ttccgcgttt ctacgctgga tctggaaaat ctgccgcgta acgatcaggg caaagtggat
     9181 ttcgacaaag acttctttgg taaagagtct ttcctgaccg tatctggcca gttgaacggc
     9241 gaaacctacg cttgcgcatt atccaaaatt tataccttcg gcccgacttt ccgtgctgaa
     9301 aactccaaca ccagccgtca cctggcggaa ttctggatgc tggagccgga agtggcgttt
     9361 gctaacctga acgatatcgc gggtctggct gaagccatgc tgaaatatgt cttcaaagcg
     9421 gttctcgaag aacgcgctga cgacatgaaa ttcttcgctg aacgcgtaga taaagatgcc
     9481 gtttcacgtc tggaacgctt tattgaggcc gatttcgcgc aggtggatta caccgaagca
     9541 gtaaccattc tcgaaaactg cggcaggaag tttgaaaacc cggtttactg gggcgtcgat
     9601 ctctcttctg agcatgagcg ttatctggcg gaagaacact ttaaagcacc ggtagtggtt
     9661 aaaaactatc cgaaagatat taaagcgttc tatatgcgcc tcaacgaaga cggtaaaacc
     9721 gttgcggcta tggatgttct ggctccgggc atcggtgaga tcattggtgg ctcccagcgt
     9781 gaagagcgtc tggacgtgct ggacgagcgt atgctggaaa tgggcctgaa caaagaagat
     9841 tactggtggt atcgcgatct gcgtcgctac ggtactgttc cgcattccgg tttcggtctt
     9901 ggttttgaac gcctgattgc ttacgtaact ggtgtgcaaa acgtgcgtga tgtgattccg
     9961 ttcccacgaa ctccgcgtaa cgccagcttc taatttagcg tctacaagag ccagcacccg
    10021 ctggctcttt tttatccttt ctgtcaagtt atctgtttgt taagtcaatc aatctatttg
    10081 caaccccgcc ataacgatca tcctgttacg gaatattaca ttgcaacatt tacgcacaaa
    10141 aaataacccg cattcttatt gcggattagt tttttcttag ctgatagcac aattttcata
//
LOCUS       DBFIFM010000166        10070 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.166, whole genome shotgun sequence.
ACCESSION   DBFIFM010000166 DBFIFM010000000
VERSION     DBFIFM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.166"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..1774
                     /gene="gspD"
                     /locus_tag="N7C74_003637"
     CDS             <1..1774
                     /gene="gspD"
                     /locus_tag="N7C74_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HGU2433433.1"
                     /translation="VRSYDMLNEEQYYQFFLSVLDVYGFAVVDMHNGILKVVRSKDAK
                     TSAVPVASDVSPGTGDEVVTRVVPVSNVAARDLAPLLRQLNDNAGAGSVVHYEPSNVL
                     LMTGRAAVMKRLMEIVERVDKVGNRSVATVPLTYASATDVARLVTELTKETDKTAIPA
                     WMTAKLVADERTNSVLVSGEPISQQRIISIIKQLDRQEDVQGNTKVIYLKYAKAKDLV
                     EVLTGISSSIENDSKKSPSTEALRKGVTIKSHEQTNALILTGAPDVIRDLENVISQLD
                     IRRPQVLVEAIIAEIQDADGLNLGIQWVNKHAGVAQFTSTGLPITTMVQTRQNEILDS
                     DQSNALSMFNGIAAGFYQGNWAMLLTALSTSSKNDILATPSIVTLDNMEATFNVGQEV
                     PVLSGSQTTSGDNIFNTVERKTVGIKLRVKPQINEGDSVLLEIEQEVSGVADTAVATT
                     TDLGATFNTRTVTNAMLVGNGETVVVGGLLDKSIRGSESKVPLLGDIPVLGHLFRAKS
                     EQTAKRNLMLFIRPTIIRERDGFRHASAEKYQSFNQEQVQSRGKETTALTLNEEQLRL
                     SPDQDDTAFRKVKAAIAAFYAQEM"
     gene            1774..3279
                     /gene="gspE"
                     /locus_tag="N7C74_003638"
     CDS             1774..3279
                     /gene="gspE"
                     /locus_tag="N7C74_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HGU2433434.1"
                     /translation="MSRVVQNVSESRPLLPFSFSRTQRILLLREQEGNRVFCMEDTPA
                     SALLEVRRVAEGPLNVTTVSAEAFEKQLVSSYQRDSDEARQMMAEIGNEMDFYTVAGE
                     LPDREDLLDANDDAPIIRLINAMLTEAIKEKASDIHIETYERHLQVRFRIDGVLREIL
                     RLHRNLASLLISRIKVMARLDIAEKRVPQDGRMVLRIGGRAVDVRVSTLPSNHGERIV
                     LRLLDKNSVSLDLAALGMSQQNQRHIDALIRRPHGIILVTGPTGSGKSTTLYAALSLL
                     NPRDRNIMTVEDPVEYELDGISQTQVNPKVDMTFARSLRAILRQDPDVVLVGEIRDGE
                     TAQIAVQASLTGHLVLSTLHTNSAAGALSRLQDMGIPPFLLSTSLLAVLAQRLVRTLC
                     PRCRQPCQVSTELAMDMDIPPETTIWQPAGCQHCSFTGYHGRTGIHELLLIDDRIRTA
                     IYQGEGELGITRLAGSRYLTLRGDGRQKVLAGETSWEEVVRVTESRLQEEE"
     gene            3281..4504
                     /gene="gspF"
                     /locus_tag="N7C74_003639"
     CDS             3281..4504
                     /gene="gspF"
                     /locus_tag="N7C74_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HGU2433435.1"
                     /translation="MALFHYQASDIHGRKRSGILEADSARHARQLLREQALIPVRLDE
                     KQVHHKHSLRSILRFRPRGGSSAELALLTRQLATLVAASLPLEEALDALLRQSEKPRQ
                     RNLIAAVRTKVLEGHSLAAAMGMFPGTFERLYCAMVAAGETSGRLDVVLSRLADYTEQ
                     RQIMRNRLLQALLYPCVLTLVAVGVIAILLTAVVPKVVEQFIHMKQTLPLSTRVLMGA
                     AEVSQTWGPWLLLAAALGGIAGRMILHQPSQRLAFHHLLLRLPVVGRISRGLNTARYA
                     RTLSILNASAVPLLQAMHISGDVLSNDWARHQLATAAELVREGVSLHQALEQTSLFPP
                     MMRHMIASGENSGELDSMLERAADNQDREFSTQMQLALGLFEPLLVVGMAGVVLFIVL
                     AILQPLLQLNNMMNM"
     gene            4535..4969
                     /gene="gspG"
                     /locus_tag="N7C74_003640"
     CDS             4535..4969
                     /gene="gspG"
                     /locus_tag="N7C74_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HGU2433436.1"
                     /translation="MRKQHQRGFTLLEIMVVIVILGVLASLVVPNLMGNKDKADRQKV
                     MSDLVALESTLDMYRLDNNRYPTTEQGLRALVSKPTVQPEPRNYRQDGYIRRLPQDPW
                     GGDYQLLNPGQYSDIDIFSPGPDGVPNTEDDIGNWTLGNAQP"
     gene            4966..5520
                     /gene="gspH"
                     /locus_tag="N7C74_003641"
     CDS             4966..5520
                     /gene="gspH"
                     /locus_tag="N7C74_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HGU2433437.1"
                     /translation="MSQRGFTILEMMLILLLMGITAGLVLMSFPDSAQNHLQQQRERL
                     QAQLDYALDRSQQDGLLMGIQVRFDGWKFKVLQRGTAESPPTLAEGGDIWQGYVWQTW
                     QPRRAAMGGKLPDDVRLELQYLRGLQWMSEHDDGAEPDILLLPGGEVTPFRLLFRQVG
                     EEAVVGLQVDENGLMTLFEGEVSL"
     gene            5517..5882
                     /gene="gspI"
                     /locus_tag="N7C74_003642"
     CDS             5517..5882
                     /gene="gspI"
                     /locus_tag="N7C74_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HGU2433438.1"
                     /translation="MKGQEGMTLLEVIVALVVFALAGMALMQASTQQAAGIGRMEEKV
                     LAGWLADNQMVQLQLEKTWPENGWGEKTISFAGTEWYLRWGGPDSDVPQPRSLEVEVR
                     RTKEETSALVSLRSSVVRE"
     gene            5879..6478
                     /gene="gspJ"
                     /locus_tag="N7C74_003643"
     CDS             5879..6478
                     /gene="gspJ"
                     /locus_tag="N7C74_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HGU2433439.1"
                     /translation="MSQQRVKGFTLLEMLLALAVFAALSISAFQVLQGGIRAHELSRD
                     KVQRLAELQRGISQMERDLTQMLPRHSRGNEGLLLAAPHLLKSDDWGISFTRNSWLNP
                     AGMLPRPELQWVGYRLRQQKLERLSYFHVDHPSGVSPDVRVMLDGVHAFRLRFFVNGD
                     WQARWDSTGILPQAVEVTLVMDDFAELPRLFLVSKETAE"
     gene            6475..7452
                     /gene="gspK"
                     /locus_tag="N7C74_003644"
     CDS             6475..7452
                     /gene="gspK"
                     /locus_tag="N7C74_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HGU2433440.1"
                     /translation="MKLREQGVALLVVLLILSLMVTIAAVIAERNGRTFLRTVAQLDQ
                     LQAKWDGYTAETIAKQILQRSRQESPRKTHLAQNWAQSERQFETRGGDVRGQIVDAQA
                     CFNLNAINYGVVDLTSIPYAARIFQQLLINLQVELLQARQVTAALRDWIDRDDKPVRG
                     GAEDEVYMGMEPPYLAANQPMQDVSELRLIRGIDARLYRKLLPYVCVLPTSDLSVNVN
                     TLLDSQAPLLAALFLTKPDSLPVTELLQRRPRTGWESVAAFLDAPPLKDIDTSAAMPV
                     LAVSSNYFLVRLHVRSGEHLFSQQTLMQWREERFRIIQRQYGLTMREVP"
     gene            7386..8663
                     /gene="gspL"
                     /locus_tag="N7C74_003645"
     CDS             7386..8663
                     /gene="gspL"
                     /locus_tag="N7C74_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HGU2433441.1"
                     /translation="MEGGAFPDHSATVWINDEGGSLKKNNKHVEAGRLFLRLPEDDSE
                     PLLWRLIAPEETGAGSVAVGETDPQLVGLLSRYPAWVLVPSGRIAFHQVTLPHRTWRQ
                     RLQVLPFLLEEQLATDIEQLHFAILHQSGDRCDVAVVEKAVMHRWLAYCGQLGVREVR
                     LLPDVLMLPLATEGWSAVKLAGQWLFRRDRYAGMTVELSWLTHFLSLTAPPVIESYSV
                     PPVPCPGPKTTEWRNQPGRDLLQLVAEGDGYDGADLRQGEFARQGTWYARFRPWRYVA
                     GALLACVFLAGSNAGLAHYRLWQQAQFWRYESVRVYQQLFPSEKAVKDPRRQMLRHLQ
                     QSTGNNIPELGSVMRQLQQLLSETSTIRLQALAWDSSSQTLKIDLQAASFQALEHFQQ
                     IAGEKYLIQPGEIRQQPQGVESRLILRVNNERA"
     gene            8650..9162
                     /gene="gspM"
                     /locus_tag="N7C74_003646"
     CDS             8650..9162
                     /gene="gspM"
                     /locus_tag="N7C74_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HGU2433442.1"
                     /translation="MNELKKRLQYVSPRERRLLIGCVALLVVVFVYYVLWQPWLIRED
                     KWRTVVTREKNTVEWMKQQVPNIKRREASMPEQGEPLGLSAAVTRTSAAYGVSVTRLQ
                     PQGERLAVTLAPVEFNALMQWLTQLERRHRIRTMVFDVAAQGNPPGQVTVNRLVLSQD
                     DVNKDASLSR"
     gene            9283..10053
                     /locus_tag="N7C74_003647"
     CDS             9283..10053
                     /locus_tag="N7C74_003647"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HGU2433443.1"
                     /translation="MGGVTGSFLNVVIHRLPIMLERRWRREALTLLAHSVPESGPAFN
                     LMIPQSHCPCCFHPLGIRDNIPLLSYLFLKGKSRCCGERIPVYYPLVEITNSVLFILA
                     ASRFPPGLTLAAAWLFISMLLVLAVIDCHTALLPDVLTLPLLWLGLLFSLQRGVVTLE
                     EAVVGAVSGYLCLWGLYWLFRFATGREGLGYGDFKLAAALGAWMGWQALPSILLFASV
                     SGLIVTVLLRIVTATDFTRPLPFGPWLALSGVCHFLLM"
BASE COUNT         2219 a         2332 c         3092 g         2427 t
ORIGIN      
        1 tgtacgcagc tacgatatgc tgaacgagga acaatattat cagttctttc tcagtgtgct
       61 ggacgtttat ggttttgctg tggtcgatat gcacaacggt atactgaaag tagtgcgctc
      121 aaaagatgcc aaaacgtcgg cggtgccggt agctagtgat gtcagtcccg ggactggtga
      181 tgaggttgtt acccgggtgg tccccgtaag taacgtggca gccagagatc tggcgccttt
      241 gctgcgtcag ctcaatgata atgctggcgc aggaagcgtg gtgcattatg aaccttctaa
      301 tgttttgttg atgaccggac gtgctgcagt gatgaaacgg ttgatggaga ttgttgaacg
      361 tgtggataag gtgggtaatc gcagcgttgc cacggtcccg ctcacctacg cgtccgcaac
      421 agacgtagcc agacttgtta cggaactgac taaagaaaca gataagacag ctatacctgc
      481 ttggatgacg gcgaaactgg ttgcagacga gaggacaaac tcagtgctcg tcagcggaga
      541 gccaatctcc caacagcgta tcatctccat aattaagcaa ctggatcgtc aggaggatgt
      601 tcagggtaat actaaggtga tttacctgaa atatgcgaag gcgaaggatt tagtggaagt
      661 cctgacaggt atcagcagca gtattgaaaa cgactctaaa aagagtccgt caacggaagc
      721 cttgcgcaaa ggagtgacga ttaaatccca cgaacaaacc aatgccctga tcctgacggg
      781 ggcccctgac gtcatccgcg accttgaaaa tgtgatttcg cagttggata ttcgtcgtcc
      841 tcaggtcctg gtggaggcca tcattgctga aatacaggat gctgacgggc tgaaccttgg
      901 gatccagtgg gtgaataaac atgccggtgt ggcgcagttc accagtaccg gtttacctat
      961 taccacgatg gttcagactc gtcagaacga aatcttagac agcgatcaga gcaatgccct
     1021 gagcatgttt aacggaattg cagcggggtt ttatcaggga aactgggcga tgctgttgac
     1081 ggcgctctcc acaagtagca agaatgatat cttggcgacc cccagtattg tcacgctgga
     1141 caatatggag gccactttca atgttggtca ggaggtcccg gtactttcgg gctcacagac
     1201 aacctctggg gacaatattt ttaacacggt cgagcgcaaa acggtgggga tcaaactcag
     1261 ggtaaaaccc cagatcaacg agggtgattc cgtgttactg gagatagaac aggaggtgtc
     1321 cggtgtggcg gacactgcag tagccaccac tactgacttg ggagcaacct tcaacacccg
     1381 aacagtgacc aatgccatgc tggtcgggaa tggcgaaacg gtggtggtcg gaggattact
     1441 ggataagtcg atcaggggga gtgagagtaa agtgccactg ctgggggata tcccggtact
     1501 ggggcatctt tttcgcgcaa aaagcgaaca gacagctaag cgtaatctga tgctgttcat
     1561 tcggccaact attattcgtg agcgcgacgg atttcgtcat gcttcggccg aaaaatacca
     1621 gtcgtttaat caggaacagg tgcagtcgcg tggcaaagaa acaacggcgc tgacgctgaa
     1681 tgaggaacag ctcaggctgt cccccgatca agacgatacg gctttccgga aggtgaaagc
     1741 ggcgattgct gcgttttatg cgcaggagat gtaatgagca gagtagtaca gaatgtcagt
     1801 gaatcacgtc cccttctgcc tttttccttt tcccgtacgc agagaatttt gttgctgcgt
     1861 gaacaggagg ggaatcgagt gttctgcatg gaggacacac ctgccagtgc cctgttggag
     1921 gtgcgcaggg tggctgaagg tcctctgaat gtcacaacag tgtcggcgga agcgtttgaa
     1981 aaacagctgg tgagcagcta tcaacgtgat tctgatgaag cgcgacaaat gatggctgag
     2041 attggtaatg aaatggattt ttataccgtc gcaggggagt tgcctgaccg tgaggactta
     2101 ctggatgcga atgacgatgc gccaattatc cgcctgatta acgcaatgct gacagaggca
     2161 attaaagaga aagcctcaga tattcatatt gaaacttacg aacgccatct gcaggttcgc
     2221 tttcgcattg atggcgttct gcgggagatc ctccgtttac ataggaatct ggcttcgtta
     2281 ctgatttcac gcattaaagt catggcccgg ttggatattg ccgaaaaacg cgttccccaa
     2341 gatggccgca tggtactgcg tatcggtggt cgggcggtgg atgtgcgtgt ttcaacgttg
     2401 ccttcaaatc atggtgaacg catcgtgctg cgtttactag acaaaaatag cgttagtctc
     2461 gatcttgctg cactcggcat gtcgcagcag aatcagcgac acattgatgc actgatccgc
     2521 cgtcctcacg gcattatttt ggtgaccggg cctacagggt cgggtaaaag cacgacgctt
     2581 tatgccgcgt taagcctgct gaatccccga gaccgcaaca ttatgacggt cgaggatcct
     2641 gttgaatatg aactggacgg tatcagtcag acccaggtca acccgaaggt ggacatgacc
     2701 tttgcccgga gtctgcgcgc tattttacgt caggatccgg acgtggtgct tgtgggggag
     2761 atccgtgacg gtgaaacagc ccagattgct gtgcaggcct cgctgacagg gcatttggtg
     2821 ctgtcaacgt tacatacgaa tagtgcagca ggcgcgctgt cgcgtctgca ggatatgggc
     2881 atccctcctt ttctgctttc cacctcatta ctggctgttc tggcccagcg actggttcgc
     2941 acgttgtgtc cgcgctgtcg tcagccctgt caggtcagta ctgagttagc gatggacatg
     3001 gacattcccc ctgaaaccac gatctggcag cctgccgggt gtcagcactg cagtttcacg
     3061 ggctatcacg ggcgcaccgg gatccacgag ctgttgctga ttgacgatcg cattcggacg
     3121 gcaatctatc agggggaggg ggagctgggt attacccgct tggcagggag tcgctatctg
     3181 acgctgcgtg gcgacgggcg acaaaaggta ctagccggtg agaccagttg ggaggaggtg
     3241 gttcgcgtta ctgaaagcag attgcaggaa gaggaatgat atggcgttgt ttcattatca
     3301 ggcatcagat attcacggta gaaaacgcag tggcattctg gaggcggatt cggcccgaca
     3361 tgcccgtcag ttgctgcgtg agcaggcgct tattccagtc aggttggatg agaaacaggt
     3421 ccatcacaag cactcactgc ggagtatcct gaggtttcgt ccgcgcgggg ggagcagcgc
     3481 cgaactcgcg ttactgacac ggcagttagc cacactagtg gccgcatctc tgccgcttga
     3541 agaggcgctg gatgcgttgt tgaggcagag tgaaaaaccg cgtcagcgga acttaatcgc
     3601 tgccgtgcgc accaaggttc tcgagggaca ttccttggcc gcggcgatgg gcatgtttcc
     3661 aggtacgttt gagcgtcttt attgtgcgat ggtggcggcg ggggaaacat ccggtcgttt
     3721 ggacgtggtg ctcagtcgtc tggctgacta taccgagcag cgccagatca tgcgaaaccg
     3781 tctactccag gcattgctct atccttgtgt tctgacgttg gtggcggttg gggttattgc
     3841 cattctgctt actgcggttg tgccgaaagt ggttgagcag tttattcata tgaaacagac
     3901 ccttccctta tctacccgcg tactgatggg ggctgctgag gtgagccaga cttggggccc
     3961 gtggctgtta ctggccgcag cgctgggcgg gatagcgggg cgcatgatat tacatcagcc
     4021 ctcccagcgt ctggcttttc atcatctgct gttgcggctg ccggtcgtgg ggcgcatatc
     4081 gcgcggtctg aataccgcac gttatgcccg cacgctgagc attcttaatg ccagtgcggt
     4141 accgctgctc caggcgatgc acatcagtgg tgacgtactg agtaatgact gggcccgtca
     4201 tcagttagct accgcggccg agttggttag ggaaggggtc agcctgcatc aggcactgga
     4261 gcagacttcg ctgtttccgc ctatgatgcg gcacatgatt gccagcggtg aaaatagtgg
     4321 cgaactcgac agcatgttgg aacgggccgc cgacaatcag gatcgtgagt tcagcacaca
     4381 gatgcaactg gcgctgggat tgtttgaacc gctgttggtg gtgggtatgg ccggggtcgt
     4441 tttgtttatt gttctggcaa ttctgcagcc gctcctgcag ctcaacaaca tgatgaatat
     4501 gtgaatcctt ctttaacagt caacggagaa aaacatgcga aaacagcatc agcgtggttt
     4561 taccttgctg gaaataatgg tggtaattgt gatcttggga gttttggcca gtctggtggt
     4621 cccgaatctg atgggaaata aggataaggc cgatcggcag aaagtgatga gcgacttggt
     4681 tgcgctggaa agcacgctcg atatgtaccg gttagataat aaccggtatc cgacaacgga
     4741 gcagggactg cgggcactgg tcagtaaacc caccgtgcaa cctgaacccc gtaactaccg
     4801 gcaggatggt tatatccgcc gtttgcccca ggatccttgg gggggggatt atcagctgtt
     4861 aaatcctggt cagtacagcg acattgatat cttttctcct ggcccggatg gtgtgccaaa
     4921 cactgaagat gatattggta actggacgct cggtaacgcg cagccatgag tcagcgcggt
     4981 ttcacaatac tggagatgat gctgatcctt ttgttgatgg gcatcactgc agggctggta
     5041 ctaatgtcat ttcctgactc tgctcagaat catctgcaac aacagcggga gcgtctgcag
     5101 gcgcagctgg attatgcctt ggatagaagc cagcaggacg gcttgctgat ggggattcag
     5161 gttcggtttg acggatggaa atttaaggtt ctgcagcgcg gaacagcgga aagccccccg
     5221 acattagctg agggagggga tatttggcag gggtatgtct ggcaaacttg gcaaccgcga
     5281 cgtgcagcca tggggggcaa acttccggat gatgtgcgtc ttgagctgca gtatctcaga
     5341 ggtttgcagt ggatgtctga gcatgatgac ggggctgaac ccgatatttt gctgcttccc
     5401 ggaggtgagg taacgccgtt cagacttctt ttcaggcagg tcggagagga ggccgttgtc
     5461 gggctgcagg ttgatgagaa cggtttgatg actctttttg aaggtgaggt ctcattgtga
     5521 aggggcaaga aggaatgaca ttactggaag tcatagtagc tctggttgtg tttgcgctgg
     5581 caggaatggc gctgatgcag gcctcgaccc agcaggctgc ggggatcgga cggatggaag
     5641 agaaggtgct ggcaggctgg ttggccgata accagatggt gcaattgcaa ctcgagaaaa
     5701 cttggccgga aaatggctgg ggcgagaaga cgatcagctt cgctggcacc gaatggtatt
     5761 tacgctgggg cgggccggac agcgacgtgc cgcagccgcg ctccttagag gtagaggtac
     5821 ggcgcactaa agaggagacg tccgccttgg tttccttgcg tagcagtgtg gttcgtgaat
     5881 gagtcagcaa cgggtaaaag gttttacgct actggaaatg ctgttggcac tggctgtttt
     5941 tgcagcgttg agcatcagcg catttcaggt gctgcagggt ggaatacggg cgcacgagct
     6001 gtcccgggat aaagtacagc gtctggctga gctgcagaga gggatcagcc agatggagcg
     6061 agatcttacg cagatgctcc caaggcacag ccgggggaat gaaggcctgc tgctcgctgc
     6121 acctcatctg ctaaaaagtg atgactgggg gatttctttt acgcgaaaca gctggctgaa
     6181 tcccgccggg atgctgccgc gcccggagtt acagtgggta ggttatcggt tgcgacagca
     6241 aaagttagaa cgcctgagct actttcatgt cgatcaccct tccggtgttt ctccggatgt
     6301 cagggtgatg ctggacggcg ttcacgcatt tcgtctgcgt ttttttgtta acggtgattg
     6361 gcaggcgcgc tgggacagta ccggcatcct gccgcaggct gttgaagtga cgctggttat
     6421 ggatgatttt gctgaactgc cccgtctgtt tcttgtgagc aaggagaccg ctgaatgaaa
     6481 ttgcgggaac agggcgtggc gcttctggtg gtattgctga tcctgtcgtt aatggtaacg
     6541 atagcggcgg tgattgcgga gcgcaacggt cggacatttt tgcgtacagt agcgcagttg
     6601 gatcagctgc aggcaaagtg ggatggctat acggcagaga cgatagcaaa acaaatctta
     6661 cagaggagca ggcaggagtc gccccggaaa acgcatctgg cacaaaattg ggcgcagtca
     6721 gaacgacagt ttgagaccag agggggagat gtgcgggggc agatcgtgga tgcgcaggcc
     6781 tgttttaatc ttaatgctat taattatgga gtggtggatc tgaccagtat accttatgcc
     6841 gccagaattt ttcagcagct attaatcaac ttgcaggtag agcttttaca ggcccggcaa
     6901 gtgacggcag ccctacgtga ctggatcgat cgtgatgata agccggtaag gggcggtgct
     6961 gaagacgagg tgtatatggg gatggaacct ccctatctgg cagccaacca gccgatgcag
     7021 gacgtcagtg agttacgcct tatccgggga atagatgcca ggctttaccg caaacttttg
     7081 ccgtatgttt gtgtactgcc tacgtcagat ctgtctgtga atgtgaatac tctgctggat
     7141 tcacaggcgc cgctgctggc ggcgctcttt ctgacgaaac cggacagtct tccggtgacg
     7201 gaattactgc agcggcgacc ccgtactggt tgggagagcg tggcggcgtt tcttgacgcg
     7261 ccaccgctta aggacattga caccagtgcg gccatgccgg tattggccgt cagcagcaac
     7321 tattttctgg ttcggttaca tgtgcgctcc ggggaacacc tgttcagtca gcagacttta
     7381 atgcagtgga gggaggagcg tttccggatc attcagcgac agtatggatt aacgatgagg
     7441 gaggttccct gaaaaaaaat aacaaacatg tcgaggctgg tcggcttttt ctgcgactgc
     7501 cggaggatga ctcggagcct ttattgtgga ggctgattgc gccagaggag acaggagccg
     7561 gcagtgtggc agtcggagaa acggatccgc agttagtcgg tttgctgtcc cgttatcccg
     7621 cctgggtgct ggttccgtca ggcagaattg ccttccatca ggtcacgctg ccacaccgca
     7681 cgtggcgtca gcgtttgcag gtgctgccct ttcttctgga agagcagttg gcgacagaca
     7741 ttgagcagct gcacttcgcc attcttcatc agtcgggtga caggtgtgat gtggcggtgg
     7801 tcgaaaaagc ggttatgcat cgctggttag cgtactgtgg acaacttggg gttcgggagg
     7861 tgaggttgct gcctgacgta ctgatgctgc cgttggcaac tgaaggatgg agtgccgtaa
     7921 agcttgccgg gcagtggctg tttcgccggg atcgttacgc cgggatgacg gtagagttat
     7981 cgtggctgac acatttcttg tctcttactg cgccgccggt gattgagtcc tactccgtgc
     8041 cgccagtacc gtgcccgggt ccgaagacca ctgagtggcg aaatcagccc ggacgcgatc
     8101 tgctgcagtt ggtggccgaa ggtgacgggt atgatggcgc agatctgcgg cagggagaat
     8161 ttgcccgcca gggaacatgg tatgcacgat ttcgcccatg gaggtatgtc gcaggggctc
     8221 tgctggcctg tgtttttctg gccgggagca acgcggggct ggcacattat cggctctggc
     8281 agcaggctca attctggcgg tatgagagcg tacgtgtcta tcagcagctg tttccgagtg
     8341 aaaaggcggt gaaggatccc cgccggcaga tgctgagaca tctgcaacag tcgacgggta
     8401 acaatatacc ggaactcggg agcgtgatgc gccagcttca gcaactgctg tcagaaacct
     8461 caacgatacg cttacaggca ctcgcctggg acagcagcag ccagacactg aaaattgatt
     8521 tgcaggcggc ttcttttcag gcgctggaac actttcagca gatcgccgga gaaaaatacc
     8581 tcatccagcc tggtgagata cgacagcagc cgcagggggt agaaagtcgt ctgatcctga
     8641 gagtgaacaa tgaacgagct taaaaaacgt ttgcagtatg ttagcccccg tgagcgcagg
     8701 ctgcttatcg gatgtgttgc cttactggtt gttgtttttg tctattacgt cctctggcag
     8761 ccctggctga tacgagagga taagtggcgg actgtagtta cccgtgaaaa aaacacagtg
     8821 gagtggatga aacaacaggt acccaacata aagagacggg aggcaagtat gcctgaacag
     8881 ggggagccgt taggtttatc tgcggccgtg acacgtactt cagccgcata cggtgtatcg
     8941 gtcacccggt tgcagcctca aggggagcgt ctggccgtca ccttagcccc ggttgaattt
     9001 aatgcgctga tgcagtggct tacgcaactg gagcgtcggc accgtattcg taccatggta
     9061 tttgatgtgg ccgcgcaggg caacccaccc ggacaggtaa cggttaatcg gctggttttg
     9121 tcccaagatg acgtaaacaa ggacgcttcg cttagtcgct gatgtcgtaa tttacagatt
     9181 aatgcttgat gtaaaaattg gacttataaa ggaggatgtg tgagtagttt gccggaattt
     9241 gcggttgatt atcccttgtt gtggctcatc tgcaccggag ggctgggggg cgttaccggc
     9301 agttttctta acgtcgtgat tcatcgtctg ccgataatgt tggagaggcg gtggcggcgt
     9361 gaagcgctga cgctattggc acattctgtg cctgagtccg ggccggcctt taacctgatg
     9421 ataccgcagt ctcattgtcc ctgctgtttt catccgctcg gcatcaggga taacattccc
     9481 ctgttaagct acctatttct gaaggggaaa tcacgctgtt gtggcgagcg tattcccgtt
     9541 tactacccgc tagtcgaaat aacgaactca gtgttattca ttctggccgc gagcaggttt
     9601 ccaccagggc tgacattagc ggctgcctgg ctttttattt ccatgctgtt ggttctggcg
     9661 gttattgact gccatacggc gctgctgccg gatgtgctga cgttgccatt gctctggctt
     9721 ggcctgttat tcagtttaca gagaggcgtt gtcaccttag aggaggcggt agtgggggcg
     9781 gtatctgggt atctctgtct gtggggactg tactggcttt ttcgtttcgc taccgggagg
     9841 gaagggttag gttacggtga ttttaagcta gctgcggcat tgggagcatg gatgggttgg
     9901 caggcattac cctcaatact tttgtttgcc tcagtcagtg ggttaatcgt aacagtactg
     9961 ttgcgtattg taacagctac ggactttacc cgcccgctgc cttttgggcc gtggctcgct
    10021 ttgagcggag tgtgccattt tttgttgatg tagcgttcgg ttaccggggc
//
LOCUS       DBFIFM010000167         9900 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.167, whole genome shotgun sequence.
ACCESSION   DBFIFM010000167 DBFIFM010000000
VERSION     DBFIFM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.167"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            240..863
                     /gene="rcsA"
                     /locus_tag="N7C74_003648"
     CDS             240..863
                     /gene="rcsA"
                     /locus_tag="N7C74_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HGU2433444.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(907..1095)
                     /gene="dsrB"
                     /locus_tag="N7C74_003649"
     CDS             complement(907..1095)
                     /gene="dsrB"
                     /locus_tag="N7C74_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HGU2433445.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            1258..1485
                     /gene="yodD"
                     /locus_tag="N7C74_003650"
     CDS             1258..1485
                     /gene="yodD"
                     /locus_tag="N7C74_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HGU2433446.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            1783..2598
                     /gene="yedP"
                     /locus_tag="N7C74_003651"
     CDS             1783..2598
                     /gene="yedP"
                     /locus_tag="N7C74_003651"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HGU2433447.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(2595..4289)
                     /gene="dgcQ"
                     /locus_tag="N7C74_003652"
     CDS             complement(2595..4289)
                     /gene="dgcQ"
                     /locus_tag="N7C74_003652"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HGU2433448.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHIVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(4460..4642)
                     /gene="yodC"
                     /locus_tag="N7C74_003653"
     CDS             complement(4460..4642)
                     /gene="yodC"
                     /locus_tag="N7C74_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HGU2433449.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(4721..5638)
                     /gene="yedI"
                     /locus_tag="N7C74_003654"
     CDS             complement(4721..5638)
                     /gene="yedI"
                     /locus_tag="N7C74_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HGU2433450.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQVH"
     gene            5811..6731
                     /gene="yedA"
                     /locus_tag="N7C74_003655"
     CDS             5811..6731
                     /gene="yedA"
                     /locus_tag="N7C74_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HGU2433451.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(6720..7190)
                     /gene="vsr"
                     /locus_tag="N7C74_003656"
     CDS             complement(6720..7190)
                     /gene="vsr"
                     /locus_tag="N7C74_003656"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HGU2433452.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLKDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(7171..8589)
                     /gene="dcm"
                     /locus_tag="N7C74_003657"
     CDS             complement(7171..8589)
                     /gene="dcm"
                     /locus_tag="N7C74_003657"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HGU2433453.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(8656..9351)
                     /gene="yedJ"
                     /locus_tag="N7C74_003658"
     CDS             complement(8656..9351)
                     /gene="yedJ"
                     /locus_tag="N7C74_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HGU2433454.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQSSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDAEDPLAQ
                     HRPLDDKRYALDHFQTKLLKLPQIMQTVRGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(9391..9671)
                     /gene="drpB"
                     /locus_tag="N7C74_003659"
                     /pseudo
     CDS             complement(9391..9671)
                     /gene="drpB"
                     /locus_tag="N7C74_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
BASE COUNT         2440 a         2615 c         2373 g         2472 t
ORIGIN      
        1 tacgaacatc ttccaggata ctcctgcagc gaaatatttg ttttaagctc actcacatat
       61 cgcaacattt actttacttt aagacaattc caggcaaatt atacaacact ttacgggata
      121 gtaagtccgc ctgaaaaatc gcgagagtgg cgcattaggt gacccatgtt gttccgttta
      181 gtcatgatga aatattcagg taaggggaat tatcgttacg cattgagtga gggtatgcca
      241 tgtcaacgat tattatggat ttatgtagtt acacccgact aggtttaacc gggtatctgt
      301 tgagtagagg ggttaaaaaa agagaaatca acgacattga aaccgttgat gaccttgcca
      361 tagcttgtga ttcacagcgc ccttcagtgg tgtttattaa tgaggactgt ttcatccacg
      421 atgcttctaa cagtcagcgt atcaagctca tcattaatca acatcccaat acgttattta
      481 tcgtttttat ggcaattgcc aatgttcatt ttgatgaata tctattggtc agaaaaaatt
      541 tattgatcag ttctaaatcg attaaaccgg aatctctcga cgatatcctt ggcgatattc
      601 tgaaaaaaga gacaacgata acctcgtttt taaatatgcc gacgttatca ttgagccgaa
      661 ccgaatcgag tatgttgcga atgtggatgg caggtcaggg aaccattcaa atctccgacc
      721 aaatgaatat caaagccaag accgtttcat cgcataaagg taatattaaa cgtaagatca
      781 aaacgcataa taaacaggtt atctaccatg tcgtccgact gacggataat gtgactaatg
      841 gtatttttgt caacatgcgc taacacattc tgactggtgg tttcccacca gtcaggctga
      901 attagattac tctgctttct ccacaaagat accgtcctga tgccctgctt cattaaagaa
      961 ccagatgccg agcgggtagt cttccagcga aaccaggtac attgtgcctt cactaaactc
     1021 ctcaactgcc agtaccacgc caggacgacg cggaccgcca tccgttttga ctgttacccg
     1081 atcattcacc ttcatcgttt tcctcctgtg gctttgtgcc agtgtagaac aatttcgttt
     1141 ttctggcagc gccaggcgcg cgcgagtgct gattttctcg acggtctata cttaagagat
     1201 gccaggcgga cttaacgact ggcggcaaca acagagtaac ggttgcgagg aaagatgatg
     1261 aaaaccgcaa aagagtacag cgataccgca aaacgtgagg tcagcgtcga tgtcgatgcc
     1321 ctgctggcgg cgatcaatga aattagcgaa agcgaagttc atcgcagcca gaacgattct
     1381 gaacacgtta gcgtcgatgg acgtgaatat catacatggc gtgaattggc ggatgccttc
     1441 gaactggata ttcatgactt cagcgtctct gaagtgaatc gttgaatgca caataaaaaa
     1501 atcccgaccc tgagggggtc gggatgaaac ttgcttaagc aagaagcact taaaaaattc
     1561 gttacaccag gaaatctgat gtgttcatca ccttatccgc aatttttttc gctgacaaga
     1621 aaatattcgc tatatgaatg attaacgcta cttatgaata gaaatgtgac gcacgtcacc
     1681 ccttggtggc tctggcgtca gcgggatcgc tcccgaaaaa atcatctttg ataatttgtg
     1741 ctgttttgag aatcatcctg gtaaggggag tactgcgcga ctatgttttc aattcaacaa
     1801 ccactactgg tttttagcga tcttgatggc accctgctgg acagtcatag ttatgactgg
     1861 caaccggcag ccccctggct cagccgttta cgcgaagcaa atgttcccgt cattctctgt
     1921 agcagtaaaa catcagcgga aatgctgtac ttgcaaaaaa tgttggggct acaaggttta
     1981 ccgctgattg cagagaatgg cgcagtgatc cagcttgctg aacaatggca ggagatagac
     2041 ggttttccac gcatcatctc aggtattagc catggcgaaa tcagccaggt tttaaatacg
     2101 ctacgcgaga aagaacattt taaattcacg acttttgatg atgtcgacga tgcaaccatc
     2161 gccgaatgga cgggattaag ccgtagccag gcggcgctga cgcagcttca tgaggcgtcg
     2221 gtaacgctaa tctggcgcga cagtgacgag cgtatggcac aatttaccgc tcgtctgaac
     2281 gaactgggct tacagtttat gcaaggtgcg cgcttctggc acgtcctgga tgcctctgcc
     2341 ggaaaagatc aggctgccaa ctggattatc gcgacctatc aacaattgtc aggcaaacgc
     2401 ccaaccacac ttggcctggg cgatgggcca aacgatgcgc ccttactgga ggtaatggat
     2461 tacgcggtga ttgtgaaagg gctaaaccgt gaaggggtgc atctgcatga tgaggatccg
     2521 gcccgcgtct ggcgaacgca gcgtgaagga ccggaaggat ggcgtgaagg gctggaccat
     2581 tttttctccg cccgttaagc gttatcgctc gcgaataccc gattgcgccc agcctgttta
     2641 gcgagataaa gccgacggtc ggccagtgac tggagttgtt caaaatcata atcaccggtt
     2701 tcctcgctgc tacttacccc caacgaggca ctgatgcgta tcgtcgtact cttagcaatc
     2761 aacatctctt tttcatttaa cttcaggcga atacgttctg cgacttccgc agcctccgtc
     2821 agactcgcgc ctggcagaat cacacaaaac tcctcaccac cgacccgccc ggcaacgtcc
     2881 tgcgcacgca aggaactgct aattaatccg gcagcatgag aaagaacacg gtcgcccgcc
     2941 tgatgaccaa agcggtcatt aatcgcttta aaatggtcaa gatcgacctg aatgacagaa
     3001 aaaggatgtt ggtgcgtctg acacaatttc gcgagcggac gggctttttc gaacagtgcg
     3061 ccacggttat ataaacgcgt taaggtgtcg tgccacgcct gccactgcaa cgagctttgc
     3121 agaacataca tgttgctgac catccggcga atcacatacc aggagatgag taacatggtg
     3181 gtaaagagcg cccacagcag ggttaatgca atgctgatac tgccgaaatc gccgcgcacg
     3241 ccttcgctta acgtatggac gcgcaccagc acaccgtcga aatggtccag acgttcccag
     3301 ctaacatagc gactgtccat acgaatgccg ccccgcgtgt catgttccat tgcctgcgcc
     3361 agcaaggcca gttcacgagg atcaaaaata ttccctgttg gatggtcagg attggaagag
     3421 gtcaaaaatc tcagcttact gtcatagagc tgatactcac catcgaggtt tttatcgatg
     3481 gcgtttctta aaaattgctg catagtacgc acgggaatac tcatccccag cacgccatac
     3541 cagtagttat tactgtctac cggaacactg acggtaacct gcggttcagt attgctggcg
     3601 tgttccggtt gcgaagtgaa ccagcgtacc gcgcggtgac gattttctcg ttgtgaatgg
     3661 ccgataaacc agggttgggt gacatagccg taataacgcg tcgggacatt gcgcgtaaac
     3721 aaggtcggct gcgtcgaaac gtaaaatccg gcacgcgaga catacatcgc ctgttctacc
     3781 atcgacgagg agttgtgtgc cagtcgcagc aagtaaccaa cttccagtgc agcggtaatt
     3841 tcattgtcga ggctttcatt ttcgcgagac aggagattcc cctcgctgac taatgcatcc
     3901 gatacgccat tgactgacag ggtgcgtcgt cggttgagtt cgatttgcca ggcgtgctcg
     3961 tcgcgatgct gttcgaactc ggttacagca tcacgcagag aggtgaaatc cagtggcgct
     4021 acgagagctt cgcgcatgcc attacgcaaa aagatcagtt tgtcgacgtt atactgcaaa
     4081 tgcttatcga gcgcgttggc tacgttttcc agatgattac gctggctgga gatataggca
     4141 tcttccagca ccactacttc acgccaggtc agcaaggtgg aaaaaagcaa taccacgata
     4201 aagcagagat taacgatatg accaggccct aggcggcgtg cgagtttttt caaccagctc
     4261 tggttttcca tttttgtctc gtgctgcacc ctgacgactc cccacctgct tttttatgat
     4321 tctggcataa cctggcgata gcgtaccgca aaacagcgat aattttataa tggtggcatg
     4381 ataagacgcg actatcattc attgcaggca ttggcacacc aggccggatg tgatccggcc
     4441 tggtcttaat gatacccttt caaacttctt ccgcagaacg actcccctcc cctggaacaa
     4501 gctcggtttc atgaaaagct tcccgcttga ccccgtaacc gtcataccaa cgacactcaa
     4561 ccataccgct ggagtatcca gtgacaatca tccgcgggcc gccctcttta accgtaactt
     4621 cctcactaac cataaagctc atactcgcct ccttttttcg ggtgaaactt gttcacctta
     4681 gttgaagatg gcgaattttg cataatagcc tgtgcgagta ttaatgtacc tgaccgcgca
     4741 ttttcgctac ggctttcact cccagcacca cgatgccgcc gatgataaat ccaagaatca
     4801 gatttaaaac agtcggtaat atcatcgcca ccactgcact ttgctgcccg gcgaaatgtt
     4861 caatggcgtg atgcagcggc gcaataccat gtaccacaat cccgccgccg acgaggaaca
     4921 tcgccagcgt gccgacaatc gataacgctt tcatcagcca gggcgcgata atcaataatc
     4981 ctttacctaa tgcctgcatc agcgcgctgg atttttccgc cagccaatac cccaggtcat
     5041 caatcttaac gataacccct accagaccgt agacgcccac ggtcactacc agcgcgatgc
     5101 ctgaaagcac cagcacctga ttaagcaacg gcgcttcggc cacaatcccc agcgtgatgg
     5161 cgacgatttc cgcagacaat ataaaatcgg tacgaatcgc cccttttatt ttgtcctttt
     5221 caaacttcag cggatcctgc gccgccagct tctccagacg ctgctggctc tgcgccggat
     5281 cttctttatg tttacgcgcc tccagcatat gcagcacttt ctctacccct tcaaagcaga
     5341 gaaacgcgcc accgatcatc aacaatggcg taatcgccca cgggataaac gcactgatga
     5401 tcagcgccag tggcaccaga atcactttat taatcagcga tcctttcgcc acgccccaga
     5461 ccacgggaag ttcccggttg gcccgcacgc ctgaaacttg ttgcgcattg agcgataagt
     5521 catcccctaa tacaccggcg gttttcttcg ccgccagttt gcccatcacg gagatatcgt
     5581 ccagcagtgt ggcgatatca tcgagcaacg tcagtaaact actcccggcc aaaagcattc
     5641 cctctcattt ttattgttga agggtgtaag tatgaagcaa aaacggcagc tctgatactt
     5701 gcttcacagg tcaacatttt tttaacaatc aagtgcaatt atatctctcg ccagcagaat
     5761 gattttcacg tttactatga accacctttt tatttccacc gtgagggatt atgcgtttcc
     5821 gccagttgtt accgcttttt ggcgcgctgt ttgcgttgta tatcatttgg ggctcaacct
     5881 attttgtcat tcggattggc gtggaaagct ggcctccgtt aatgatggcg ggtgttcgat
     5941 tcctggcagc cggtatttta ttgctggcat ttttactact gcgcggacac aaactccccc
     6001 cgctacgtcc gctgctcaat gccgcgctga ttggcctgtt attgctggct gtcggtaatg
     6061 gcatggtgac ggttgccgaa catcaaaatg ttccttccgg catcgccgcc gtagtggttg
     6121 caaccgtgcc cctctttacc ctgtgcttca gccgcctgtt tggcattaaa acgcgcaaac
     6181 tggaatgggt gggtattgcc attgggcttg ccggaatcat catgctcaat agcggtggaa
     6241 atttaagcgg caatccgtgg ggcgcgattc tgattttaat cggctcgatt agctgggcgt
     6301 ttggctcagt ttatggctcg cgcattacct tacctgtagg gatgatggcg ggtgcgattg
     6361 agatgctggc ggcaggcgtg gtgttaatga tcgcgtcgat gattgcgggt gaaaaactga
     6421 cggcgctccc ttccctttca ggcttccttg cggtcggcta tctggcgctg tttggttcga
     6481 ttatcgccat caacgcttat atgtatttaa tccgtaatgt cagtccggct ctcgccacca
     6541 gctacgctta cgttaacccg gtggtcgcgg tcttgctggg tacgggactg ggtggagaaa
     6601 cactgtcgaa gattgaatgg ctggcgctcg gcgtaattgt cttcgcggtg gtactggtca
     6661 cgttgggaaa atatctcttt ccggcaaaac ccgtagttgc tccagttatt caggacgcat
     6721 caagcgagta aatgtatccc ctgcgtgtcg atctgcgcgc tggctccttc gccgcagatc
     6781 cactcttcca gacgctcggt aagcgcttca tccttcagct tctcgcgccc acgtaacgcg
     6841 cactcccaga caatcaatac gcgccagccg agttcctgca agcgactgat atcgcggcga
     6901 tcgcgctcaa catttttacc tatcttctcc agccagaact cggttcgagt cgcaggcact
     6961 ttaaacagat agcagtgatg atgatgccag aagcagccat gggtaaatat cacgcagcga
     7021 tattcatcaa cgacaaaatc cgggcgtccg ggcagactgg cgtcctgaac gcgaaacgcc
     7081 aggccctgcc cggttaacag actggcgagg cgcttctcaa tcgccgtatc acgcgtggca
     7141 atcgcgcgca tatttttgct gcgagtggcc ttatcgtgaa cgtcggccat gttgtgcctc
     7201 ttgctgacgc aacgccaccg cctgtttgat ttttggctca agcagttttg ccaccgcggc
     7261 aaagaccggc acgaccaccg agttaccgaa ctggcgatag gcctgagtgt ccgaaaccgg
     7321 aatgcggaat ttcgcttctc ccggcgcttc aaaacccatt aagcgcgcgc attcccgagg
     7381 cgttaaccga cgtggacgat gttgctgatt cagcggatcg tcaaagtctt tctcacccgt
     7441 ggccatatcc cagccgcgat cgattaaaat ttccgcgcca tctttgtaat aacgcgcaga
     7501 cagcgtacgg gtgacgcttt gcggattgtt cggataaacc attccataac cgaagccgtt
     7561 tccgcgcgcc tgatgttttt tcgcatatcg atagaggtac ttccacagca ccggcgtcag
     7621 gatatatttc gcctcgacca tcgggtccaa cagctgcgcc agcgtcactc gctgtgcagg
     7681 gaaacattcg ctgatatcac gcagagtaaa atcggctttc agattaagat cgcgacgaaa
     7741 acccaccagc acgatgcgtt cacggtgctg cggcagaaaa tgtttgccat cgatgatttt
     7801 cggatcgtcc ggcccgttat cttctgcatc agccacgtca tagcccagtt cgtccagcgt
     7861 ctgcatgatg atgcggaacg ttttaccctg gtcgtggctt ttcaggtttt tgacgttttc
     7921 gagcacaaac atcgccggac gacgcgcgtc gataatgcgc accacatcaa agaacagcgt
     7981 gccctgagta tcgcaggcaa aaccgtgcgc ccgcccgagc gagttctttt tcgatacgcc
     8041 agccagcgaa aatggctgac aagggaaacc ggccagtaaa acatcgtgtt caggaatgtg
     8101 ttgacgaata tgttccgccg cagcctcatc actcacgcct tctttatggc tgagggtgat
     8161 gtcgcggata tcttcattaa aatgatgcgt cgccgggtcg caataatgat tggctttata
     8221 agtgcgtacc gcatgtttgt tccattcgct ggtaaacacg cactgtccgc caatcgattc
     8281 aaaaccgcga cggatgccgc caattccggc aaatagatcg ataaagcgaa acgcataatg
     8341 cggatgatgt gccggtggtt tgggtaacaa cgtttgcaga tgggcgaact ctttctcact
     8401 taaacggtgc catgccgagt cattcgccag cgcacgtttt aaaattgccg cgctccagtg
     8461 attctcaccc acaccattaa gctgagccac cagcgtttta acatcataaa tttgcagcag
     8521 tttctccagc attgcctgtg cggcattccc ggtgctgtat gaatcggtta ctgatatatt
     8581 ttcctgcata gatttaaccg gccatctgag atggtcgaca ggttaacata attacagcca
     8641 tttagggcca cgcgctcagc ccgcgggtga aaacgcatct atcaccttgt gatcgacacc
     8701 ttcattctcc cccgccagtt cggcactgag cttcgccata aactcgacta aaaaatgcgc
     8761 gttgtgctgc gccagctgct tgcctcttac ggtttgcatg atttgcggta gcttcaacaa
     8821 cttagtctgg aaatgatcca gcgcgtagcg tttatcatca agcgggcgat gctgtgcgag
     8881 cggatcttcg gcatcaaaca gcgccacgcc caatgcccct gaaacggcaa acacgcgcgc
     8941 caggccaatc gcccccaggg cttccagccg atccgcatcc tggacgattt tagcttccgt
     9001 tgttaagggg gcgatttgcg cgctgaaact gtgagcggca atggcatgac aaacggcctc
     9061 gattttctcc gccggaaatt gcacgaactc ttcacggagc aggcgacgcg tctcttctgc
     9121 cgccaggatt gaagaactct gccgttgcgg atgatttttc gccaggctca caatatcgtg
     9181 gaaataacag gcggttaaaa tcaccagcat atcgacgtca tcatctgccg ccagcttctg
     9241 tgccgtagcc cagacgcggc gaaaatgaca cacgtcatgg gccgcgtcct ggtgctgatg
     9301 atggtttttc aaccagtttt cgaactgtgc ctgccagtgt tgtaagtcca tacgcgctcc
     9361 gttgccaaag agtcgccacc ttagcagttt ttattcatag cgtctgctac gtgcgggacg
     9421 aggtcgtgtg taccaggcaa tgctgcctaa caatgccccg accagagcca taaataaaaa
     9481 tccgaccagt gccctaacca ctttcccgcg gtagagccta aatcgctttc aaatcccgac
     9541 acgggggcat cggggatccg gctcatcacc cagatgtaac gcgccattgc ccagacgaaa
     9601 tagccacacc atgtataaaa agcccacaaa gccagtttgc cgccagggct gcgagagagt
     9661 ctctcttcca tctttgtcga accgtattcc attttgagat aacccggaaa attcagactt
     9721 agtactatta cgtgataaaa gtcacatttt tccacattgg atgagccagt caagactcac
     9781 tcattttttg aacaaataga taatttttgc gcaacggcag tgaatacagg aaaaatcgtt
     9841 ttgagcgatt ttatttatct ttgagatcgg ctttattaat tcattcaatc aatatattag
//
LOCUS       DBFIFM010000168         9794 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.168, whole genome shotgun sequence.
ACCESSION   DBFIFM010000168 DBFIFM010000000
VERSION     DBFIFM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.168"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            1..687
                     /gene="yneK"
                     /locus_tag="N7C74_003660"
                     /pseudo
     CDS             1..687
                     /gene="yneK"
                     /locus_tag="N7C74_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            838..2028
                     /gene="ydeA"
                     /locus_tag="N7C74_003661"
     CDS             838..2028
                     /gene="ydeA"
                     /locus_tag="N7C74_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HGU2433455.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHLSMSMIGYVGTVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2053..2718)
                     /gene="marC"
                     /locus_tag="N7C74_003662"
     CDS             complement(2053..2718)
                     /gene="marC"
                     /locus_tag="N7C74_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HGU2433456.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNGAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TNH"
     gene            2930..3364
                     /gene="marR"
                     /locus_tag="N7C74_003663"
     CDS             2930..3364
                     /gene="marR"
                     /locus_tag="N7C74_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HGU2433457.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3384..3767
                     /gene="marA"
                     /locus_tag="N7C74_003664"
     CDS             3384..3767
                     /gene="marA"
                     /locus_tag="N7C74_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HGU2433458.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3799..4017
                     /gene="marB"
                     /locus_tag="N7C74_003665"
     CDS             3799..4017
                     /gene="marB"
                     /locus_tag="N7C74_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HGU2433459.1"
                     /translation="MKPLLSAIAAALILFSAQGVAEQTQQPLVTSCGDVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQQAM"
     gene            complement(4048..4947)
                     /gene="eamA"
                     /locus_tag="N7C74_003666"
     CDS             complement(4048..4947)
                     /gene="eamA"
                     /locus_tag="N7C74_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HGU2433460.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAISGVLVLIEDSFNGQHVAMLGFMLTLAAAFSWASGNI
                     FNKKIMSHSSRPAVMSLVVWSALIPIIPFFVASLILDGSGKMIHSLVTIDMTTILALM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMAGLYINVFGLQWRKAVKVRG"
     gene            5142..6329
                     /gene="ydeE"
                     /locus_tag="N7C74_003667"
     CDS             5142..6329
                     /gene="ydeE"
                     /locus_tag="N7C74_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HGU2433461.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTVGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMFFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLIGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAVNPLVSGVVLTTLPSSSLFIILALVIIAAWALMLKGINARPQRQP
                     ALC"
     gene            complement(6370..6765)
                     /locus_tag="N7C74_003668"
     CDS             complement(6370..6765)
                     /locus_tag="N7C74_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGU2433462.1"
                     /translation="MRYVTEFTTAKPEVAEAHFLARLSVETDCADVHDSLAYIGDDFV
                     LLHVVGREEDFARRHIPGALHLPHKQMTAERMAEWPDDTLFVVYCAGPHCNGADRAAL
                     KLARLGKPVKIMLGGITGWEDEGYAFASE"
     gene            6860..7831
                     /gene="ftrA"
                     /locus_tag="N7C74_003669"
     CDS             6860..7831
                     /gene="ftrA"
                     /locus_tag="N7C74_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FtrA"
                     /protein_id="HGU2433463.1"
                     /translation="MTTLQRPLVIALAYDGLCTFEFGVAVEIFGLPRPELGDNWYRFA
                     VAAVEPGALQATGGIHVVADGGLELLPNADMIIIPGWRGIDAKVPEMLCQSLREAHQR
                     GTRLVSICSGVFVLAAAGLLHQRKATTHWRYTEQLQHRYPSISVSDNVLYQDEGQVMT
                     SAGSAAGIDLCLHIVRKDFGHEIANNVARRLVIQPHRQGDQPQNLTRPMASPRESQTL
                     GALFDFLQQNLAQTHTVSSLAERVNMSPRTFLRRFEALTGMTPARWLLNERLIRVQDY
                     LQNSVFNIEKIAELTGFGNSALLRHHFHQRFLLSPSQYRKNSRSPSQ"
     gene            7939..8034
                     /gene="mgtS"
                     /locus_tag="N7C74_003670"
     CDS             7939..8034
                     /gene="mgtS"
                     /locus_tag="N7C74_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HGU2433464.1"
                     /translation="MLGNMNVFMAILGIILFSGFLAAYFSHKWDD"
     gene            8034..8138
                     /gene="mgtT"
                     /locus_tag="N7C74_003671"
     CDS             8034..8138
                     /gene="mgtT"
                     /locus_tag="N7C74_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HGU2433465.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(8253..9143)
                     /gene="dgcZ"
                     /locus_tag="N7C74_003672"
     CDS             complement(8253..9143)
                     /gene="dgcZ"
                     /locus_tag="N7C74_003672"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HGU2433466.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLRPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGVLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEETLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(9398..9790)
                     /gene="ydeI"
                     /locus_tag="N7C74_003673"
     CDS             complement(9398..9790)
                     /gene="ydeI"
                     /locus_tag="N7C74_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HGU2433467.1"
                     /translation="MKFQAIVLASFLVIPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPATVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         2541 a         2443 c         2198 g         2612 t
ORIGIN      
        1 ggcgtcgagc gtgtgctctt tctgtttctg aatgatttta ttgaacaatt cccaatgatc
       61 aaccctggcg ttcccatcaa aagagcgcat acaccacata ttgaacccct gcctccggat
      121 caccataccg ctgcagatta cttacgccag tttgatttgc ttgtcctgaa ttttatctct
      181 cgcggtaatt ttgtcatact cccccgatta tggaataact ctgaggttca cagatggttc
      241 gtcaataaag atcctaactt gatcaccgcc attctcgaca taacggacag cgaattaaaa
      301 gaggatttat tgcaaagcct gatggattca ttaggttcta acaaacatgt actaccctaa
      361 gtctgcatct gttttttatc ccttttagca gaacaagaat ctcctcattt tcaggacttg
      421 tttttatttt tcgccaatat gttgttacac tatcaccaat ttatgaatcc caatgaaagt
      481 gatttgaatg acgtgttgat gccagcatca ttaagtgatg ataaaattat caaacatatg
      541 gcgcgtaaga ccctcaaact gtttgtaaaa aatgaaacac cgccaaaagt tactcatgaa
      601 gatctggtga aaaacaggcc tcgctcccct gtcagaccgc ctatacccgc aacctccaaa
      661 acgccagaca ttcctgatcg tcattaaacc gtgatgttta ccatctctct gacgcgtgaa
      721 agaatcagcg tcagagaaac ggaaaacgcg atccagatca caaatgcatt gtattcacat
      781 cattaaccgt tttaagatca tttcatcact ttttcgcaac tcacccgata atctgttatg
      841 acaacaaaca ctgtttcccg caaagtggcg tggctacggg tcgttacgct ggcagtcgcc
      901 gccttcatct tcaacaccac cgaatttgtc cctgttggcc tgctctctga cattgcgcaa
      961 agttttcaca tgcaaaccgc tcaggtcggc atcatgttga ccatttacgc atgggtagta
     1021 gcgctaatgt cattgccttt tatgttaatg accagccagg ttgaacggcg caaattactg
     1081 atctgcctgt ttgtggtgtt tattgccagc cacgtactgt cgtttttatc gtggagcttt
     1141 accgttctgg tgatcagtcg cattggtgtg gcttttgcac atgcgatttt ctggtcgatt
     1201 acggcgtccc tggcgatccg tatggctccg gccgggaagc gagcacaggc attgagttta
     1261 attgccacag gtacagcact ggcaatggta ttaggattac ctctcgggcg cattgtgggg
     1321 cagtatttcg gttggcgaat gaccttcttc gcgattggta tcggggcgct tattaccctt
     1381 ttgtgcctga ttaagttact tcccttactg cccagtgagc attccggttc attgaaaagc
     1441 ctcccgctat tattccgccg cccggcattg atgagcattt atttgttaac tgtggtggtt
     1501 gtcaccgccc attacacggc atacagctat attgagcctt ttgtgcaaaa cattgcggga
     1561 ttcagcgcca actttgccac ggcattactg ttattactcg gtggtgcggg cattattggc
     1621 agcgtgattt tcggtaaact gggtaatcaa tatgcgtctg cgttggtaag tacggcgatt
     1681 gcgctgttgc tggtgtgcct ggcactgctg ctacctgcgg cgaacagtga aatacacctc
     1741 ggggtgctga gtattttctg ggggatcgcg atgatgatca tcgggcttgg tatgcaggtt
     1801 aaagtgctgg cgctggcacc agatgccacc gacgtcgcga tggcgctatt ctccgggata
     1861 tttaatattg gaatcggggc gggtgcgttg gtaggtaatc aggtgagtct gcacttgtca
     1921 atgtcgatga ttggttatgt gggcacggtg cctgcttttg ccgcgttaat atggtcaatc
     1981 attatatttc gccgctggcc agtgacactc gaagaacaga cgcaatagtt gaaaggccca
     2041 ttcgggcctt ttttaatgat tcgttttaat gatttccagg atgccgttaa taataaactg
     2101 tacacccata cataccagca ggaaccccat caagcgggag atcgcttcaa tgccaccctt
     2161 gcccaccagc cgcataattg cgccagagct gcgtagactt ccccacaaaa taaccgccac
     2221 caggaaaaag atcagcggcg gcgcaaccat cagtacccaa tcagcgaagg ttgaactctg
     2281 acgcactgtg gacgccgagc taataatcat cgcaatggtt cccggaccgg cggtacttgg
     2341 cattgccagt ggtacaaaag caatattggc gctgggttca tcttccagct cttccgactt
     2401 gcttttcgcc tccggtgaat caatcgcttt ctgttgcgga aaaagcatcc gaaaaccgat
     2461 aaacgcgacg attaagccgc ctgcaattcg cagaccggga atcgaaatgc caaatgtatc
     2521 catcaccagt tgcccggcgt aatacgccac catcatgatg gcaaagacgt acaccgaggc
     2581 catcaacgac tgacgattac gttcggcacc gttcatgttg cctgccaggc caagaaataa
     2641 cgcgacagtt gttaatgggt tagctaacgg cagtaacacc accagcccca ggccaattgc
     2701 tttaaacaaa tctaacattg gtggttgtta tcctgtgtat ctgggttatc agcgaaaagt
     2761 ataaggggta aacaaggata aagtgtcact ctttagctaa ccttgcatcg cattgaccaa
     2821 aacttgaacc gatttagcaa aacgtggcat cggtcaattc attcatttga cttatacttg
     2881 cctgggcaat attatcccct gcaactaatt acttgccagg gcaactaatg tgaaaagtac
     2941 cagcgatctg ttcaatgaaa ttattccatt gggtcgctta atccatatgg ttaatcagaa
     3001 gaaagatcgt ctgcttaacg agtatctgtc tccgctggat attaccgcgg cacagtttaa
     3061 ggtgctctgc tctatccgct gcgcggcgtg tattactccg gttgaactga aaaaagtgtt
     3121 gtcggtcgac ctgggagcac tgacccgtat gctggatcgc ctggtctgta aaggctgggt
     3181 agaaaggttg ccgaacccga atgataagcg cggcgtactg gtaaaactta ccaccagcgg
     3241 cgcggcaata tgtgaacaat gccatcaatt agttggccag gacctgcatc aagaattaac
     3301 aaaaaacctg acggcggacg aagtggcaac acttgagcat ttgcttaaga aagtcctgcc
     3361 gtaaacaaaa aagaggtatg acgatgtcca gacgcaatac tgacgctatt accattcata
     3421 gcattttgga ctggatcgag gacaacctgg aatcaccact gtcactggag aaagtgtcag
     3481 agcgttcggg ttactctaaa tggcacctgc aacggatgtt taaaaaagaa accggtcatt
     3541 cattaggcca atacatccgc agccgtaaga tgacggaaat cgcgcaaaag ctgaaggaaa
     3601 gtaacgagcc gatactctat ctggcagaac gatatggctt cgagtcgcaa caaactctga
     3661 cccgaacctt caaaaattac tttgatgttc cgccgcataa ataccggatg accaatatgc
     3721 agggcgaatc gcgcttttta catccattaa accattacaa cagctagttg aaaacgtgac
     3781 aacgtcactg aggcaattat gaaaccgctt ttatccgcaa tagcagctgc gcttattctc
     3841 ttttccgcgc agggcgttgc ggaacaaacc cagcaaccgc tcgttacttc ctgtggcgat
     3901 gtggtggttg ttcccccatc gcaggaacaa ccaccgttcg atttaaatca catgggtaca
     3961 ggcagtgaca aatcggatgc gctgggcgtg ccctattata accaacaagc catgtagtat
     4021 attcaggccc cggcatatcg gggcttgtta acctcttacc ttcaccgctt tacgccactg
     4081 caaaccaaat acattgatat acagtccggc cataatgagc acagccccta agaattgcag
     4141 acctgttaag cgttcatcca gcagtaacgc tgcacttgcc agaccgacta ccggcaccag
     4201 taatgataat ggtgcgacac gccatgtttc atagcgtcca agcagcgtac cccaaatccc
     4261 gtagccaact atcgtcgcta caaatgccag atacatcaac gccagaatgg tggtcatgtc
     4321 gatagtgacc agactgtgaa tcatttttcc ggagccatcg agaatgagcg aggcgacaaa
     4381 aaacggaatt atcgggatta atgcgctcca gacgaccaga gacattaccg ccggacggga
     4441 tgaatgagac atgatctttt tgttgaagat attgccactc gcccaactaa atgccgctgc
     4501 cagagtcaac ataaagccga gcatcgccac atgctgaccg ttaaagctat cttcgattaa
     4561 caccagtacg ccagaaatcg ctaacgcaat ccccgccagt tgcttaccgt gcagccgttc
     4621 gccaaaagta aacgcgccaa gcacgatagt aaaaaacgcc tgagcctgta agaccagcga
     4681 ggccagtcca gccggcatac cgaagttaat ggcgcagaag agaaaggcaa actgtgcaaa
     4741 actgatggtt aaaccatacc ccagcagtaa attcagcggt acttttggtc gtgccacgaa
     4801 aaagattgcc gggaaggcga ccagcataaa gcgcaaaccg gccagcatca gcggtggcat
     4861 gttatgaagc cccactttga tgaccacaaa attaagcccc catacaacga cgactagtag
     4921 cgccaacacc ccatcttttc gcgacattct accgcctctg aatttcatct tttgtaagca
     4981 atcaacttag ctgaatttac ttttctttaa cagttgattc gttagtcgcc ggttacgacg
     5041 gcattaatgc gcaaataagt cgctatactt cggatttttg ccatgctatt tctttacatc
     5101 tctaaaacaa aacataacga aacgcactgc cggacagaca aatgaactta tccctacgac
     5161 gctctaccag cgcccttctt gcctcgtcgt tgttattaac catcggacgc ggcgccacgc
     5221 tgccatttat gaccatttac ttgagtcgcc agtacagcct gagtgtcgat ctaatcggtt
     5281 atgcgatgac aattgcgctc actattggcg tcgtttttag cctcggtttt ggtatcctgg
     5341 cggataagtt cgacaagaaa cgctatatgt tactggcaat taccgccttc gccagcggtt
     5401 ttattgccat tcctttagtg aataacgtga cgctggtggt gctctttttt gccctcatta
     5461 actgcgccta ttccgttttt gctaccgtgc tgaaagcctg gtttgccgac aatctttcat
     5521 ccaccagcaa aacgaagatc ttctcgatca actacaccat gctcaatatt ggctggaccg
     5581 tcggcccgcc actcggcacg ctgttggtca tgcagagcat caatctgccc ttttggctgg
     5641 ccgctatctg ctctgcgttt cccatgtttt tcattcaaat ttgggtaaag cgcagcgaga
     5701 aaatcatcgc cacggaaaca ggcagtgtct ggtcgccgaa agttttatta caagataagg
     5761 cactgttgtg gtttacctgc tctggctttc tggcttcttt tgttagtggc gcatttgctt
     5821 catgcatttc acaatatgtg atggtgattg ctgatggtga ttttgccgaa aaggtggtcg
     5881 cggttgttct tccagtgaat gctgccatgg tggttacgtt gcaatattcc gtgggccgcc
     5941 gccttaaccc ggctaacatc cgcgcgctga tgacagcagg caccctctgt ttcgtcatcg
     6001 gtctgatcgg ttttattttt tccggcaaca gcctgctatt gtggggtatg tcagctgcgg
     6061 tatttactgt cggtgaaata atttatgcgc cgggcgagta tatgttgatt gaccatattg
     6121 cgccgccagg aatgaaagcc agctattttt ccgcccagtc attaggctgg ttgggcgcag
     6181 cggtaaatcc attagtgagt ggtgttgtac ttaccactct cccgtcgtct tcgctgttta
     6241 tcattctggc gttagtcatc attgccgcgt gggcgctgat gttaaaaggt attaacgcca
     6301 gaccccagag gcaaccagca ctctgttgac gttcgtgccg ttccactact ggcacggctt
     6361 cgctaatcat tattctgagg caaaggcata gccttcatct tcccagccgg ttatcccgcc
     6421 gagcatgatt ttaaccggct ttccgagtcg agccagtttg agtgccgcac ggtctgcacc
     6481 gttacaatgt ggccccgcgc aatacaccac aaatagcgta tcgtcgggcc attcggccat
     6541 tctttctgcg gtcatttgct tatggggtaa atggagagcg cctgggatat gccgacgcgc
     6601 gaagtcctct tcacgtccaa ccacatgaag taaaacgaaa tcatcgccaa tataagccag
     6661 agagtcatga acatcggcgc aatctgtttc gacactcaag cgcgcgagaa aatgcgcctc
     6721 agcaacttct ggcttcgctg tggtaaattc agtgacataa cgcatattgc tttgtccttt
     6781 tggtttgttg tgttaacaat aatgtaacta atctaccttt cactgaccgc ctctcgatat
     6841 gccagaaatg agcaaaatga tgacaacgtt acaacgtccg ctggtcattg cactggcata
     6901 tgacgggcta tgtacttttg aatttggcgt tgccgttgaa atattcggcc tgccacgacc
     6961 ggaactgggc gataactggt atcgttttgc tgttgctgcg gtagaaccag gtgcgctaca
     7021 agccaccggg gggatccacg tggttgctga tggtggactg gagttattgc ccaacgccga
     7081 tatgattatt attcctggct ggcgcggaat agatgccaaa gtgccggaga tgctatgcca
     7141 gtcattgcgc gaggcacacc agcgcggtac tcgcctggta tcaatatgct ctggcgtgtt
     7201 tgtccttgct gctgcgggcc ttcttcatca gcgaaaagca accacccact ggcgctacac
     7261 tgaacaactc cagcaccgtt acccttcaat cagcgtaagt gacaatgtgt tatatcagga
     7321 tgaaggacaa gtgatgacct ccgccggaag cgccgcgggg atcgatctgt gtttacatat
     7381 cgtgcgcaaa gattttggtc atgagattgc taataatgtc gcccggcgac tggtcattca
     7441 gccacatcgc cagggggatc aaccacaaaa tctgacgcgc ccaatggcct caccgcgtga
     7501 aagccagacg ttaggtgcct tattcgactt tttgcagcag aatctggcac aaacacatac
     7561 tgtgtcttcc ctcgccgaac gggtaaatat gagtccgcgc acttttttaa gacgttttga
     7621 ggcgttaact ggcatgacgc ccgcccgctg gctgctcaat gagcgcttaa tacgagtaca
     7681 ggattatctg caaaattcgg tttttaacat cgagaaaatt gccgagctga cgggttttgg
     7741 caattcagcg ttattgcgtc atcactttca ccaacgtttt ttattatctc cttcgcaata
     7801 tcgcaaaaac agccgttccc catcacaata atcgcctgat tcagcctttg tttagataac
     7861 ctctgctaat atctttctca ttgagataaa aattaaggta agcgaggaaa cacaccacac
     7921 cataaacgga ggcaaataat gctgggtaat atgaatgttt ttatggccat actgggaata
     7981 attttatttt ctggttttct ggccgcgtat ttcagccaca aatgggatga ctaatgaacg
     8041 gagataatcc ctcacctaac cggccccttg ttacagttgt gtacaagggg cctgattttt
     8101 atgacggcga aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt
     8161 gttttgcttt tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag
     8221 catcagtttt ctcaatgaat gttaaacgga gcttaaactc ggttaatcac attttgttcg
     8281 tcaataaaca tgcagcgatt tcttccggtt tgcttaccct catacattgc ccggtctgct
     8341 cttccaatga ccacatccag agtctcttca ggaaatgcgc gactcacacc tgctgtcacg
     8401 gtaatgttga tatgcccttc agaatgtgtg atggcatggt tatcgactaa ctggcaaatt
     8461 ctgacacctg cacgacatgc ttcttcatca ttagccgctt tgacaataat gataaattct
     8521 tcgcccccgt agcgataaac cgtttcgtaa tcacgcgtcc aactggctaa gtaagttgcc
     8581 agggtgcgta atactacatc gccgattaaa tgcccgtagg tatcattaac caatttaaat
     8641 cggtcaatat ccaacaacat taaataaaga ttcagaggct cagtgttgcg taactgatga
     8701 tcaaaggatt catcaagaac ccgacgcccc ggcaatcccg tcaaaacatc catattgcta
     8761 cggatcgtca gcaaataaat tttgtaatcg gttaatgccg cagtaaaaga aagcaccccc
     8821 tcctgaaagg cgtcgaaatg cgcgtcctgc cagtgatttt caacaatagc cagcattaat
     8881 tcccgaccac aattatgcat atgttgatgg gcagaatcca ttagccgaac gtaaggtaat
     8941 tcatcgttat cgagtggcct cagatgatca atccaccgac caaactggca cagtccataa
     9001 gaatggttat ccgttatttc tggcttactg gcatctctcg cgaccacgct gtgaaacata
     9061 ctcaccagcc actgatagtg ggcatcgata gccttattga gatttaacaa gatggcatca
     9121 atttccgttg tcttcttgat cattgccact cctttttcac agttccttgt gcgcgctatt
     9181 ctaacgagag aaaagcaaaa ttacgtcaat attttcatag aaatccgaag ttatgattca
     9241 tctctgagat aacattgtga tttaaaacaa aatcagcgga taaaaaagtg tttaattctg
     9301 taaattacct cttcattatc gcaaatgaaa ggatgacaaa tagcataacc cagtacccaa
     9361 atggcccggt agttcaggcc atcaggctaa tttattttta tttctgcaaa tgagtgaccc
     9421 gaacgacggc cggcgcgctt ttcttatcca gactgccgct aatgttgatc atctggtccg
     9481 gctgaacttc tcgtccatca aaaacggtcg caggaataac aacattaatt tcaccactct
     9541 tatcgcgaaa aacgtaacgg tcctctcctt tgtgagaaat caaattaccg cgtagtgaaa
     9601 ccgaagcgcc atcgtgcatg gtttttgcga aatcaacagt catttttttt gcatcatcgg
     9661 ttccgcgata gccatcttct attgcatgag gcggcggtgg cgctgcatcc tgttttaaac
     9721 cgccctggtc atctgccaac gcataaggta tgacaagaaa acttgctaat acaatggcct
     9781 gaaatttcat acta
//
LOCUS       DBFIFM010000169         9722 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.169, whole genome shotgun sequence.
ACCESSION   DBFIFM010000169 DBFIFM010000000
VERSION     DBFIFM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.169"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..773
                     /locus_tag="N7C74_003674"
     CDS             <1..773
                     /locus_tag="N7C74_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2433468.1"
                     /translation="GWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKLEKTLLIN
                     QLASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGF
                     TASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIV
                     ALCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLLGAVGGFIAPILRVKAETLF
                     ASDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            799..1593
                     /gene="yfaU"
                     /locus_tag="N7C74_003675"
     CDS             799..1593
                     /gene="yfaU"
                     /locus_tag="N7C74_003675"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HGU2433469.1"
                     /translation="MINHPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLLIDG
                     EHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTADQA
                     RQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDNLDE
                     ILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAVAPD
                     MAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(1634..2560)
                     /gene="rpnE"
                     /locus_tag="N7C74_003676"
     CDS             complement(1634..2560)
                     /gene="rpnE"
                     /locus_tag="N7C74_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HGU2433470.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLEGLQEGMHEQAI
                     KIALRMLEQGFDRDLVLAATQLSEADLAANNH"
     gene            complement(2753..3943)
                     /gene="glpC"
                     /locus_tag="N7C74_003677"
     CDS             complement(2753..3943)
                     /gene="glpC"
                     /locus_tag="N7C74_003677"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HGU2433471.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(3940..5199)
                     /gene="glpB"
                     /locus_tag="N7C74_003678"
     CDS             complement(3940..5199)
                     /gene="glpB"
                     /locus_tag="N7C74_003678"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HGU2433472.1"
                     /translation="MRFDTIIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQAVTDIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELPHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFTIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(5189..6817)
                     /gene="glpA"
                     /locus_tag="N7C74_003679"
     CDS             complement(5189..6817)
                     /gene="glpA"
                     /locus_tag="N7C74_003679"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HGU2433473.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            7090..8448
                     /gene="glpT"
                     /locus_tag="N7C74_003680"
     CDS             7090..8448
                     /gene="glpT"
                     /locus_tag="N7C74_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HGU2433474.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            8453..9529
                     /gene="glpQ"
                     /locus_tag="N7C74_003681"
     CDS             8453..9529
                     /gene="glpQ"
                     /locus_tag="N7C74_003681"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HGU2433475.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
BASE COUNT         2222 a         2405 c         2685 g         2410 t
ORIGIN      
        1 ccggctggtt ctggatgttt gtgattgaag gattgttggc agtcggcgct ggggtattca
       61 cattcttttg gcttgatgac acaccggagc aggcacgttt tctgagtaaa ctagaaaaaa
      121 cgttgcttat caatcaactg gcaagtgaag aacaacagaa agtgacttct cggctgagcg
      181 atgcgctgcg taatgggcga gtctggcaac tggcgattat ctacctgacc attcaggtgg
      241 cggtttacgg attaattttc ttcctgccga cccaggttgc ggcattgctg ggaacaaaag
      301 tgggctttac agcgtcggtg gtcaccgcca ttccgtgggt tgcggccttg tttgggacct
      361 ggcttattcc gcgctattcc gataaaaccg gcgaacggcg taatgtcgca gcgctgacat
      421 tactggcggc gggcattggt attggtctgt ccgggctgct ttctccagta ctggcgatcg
      481 tagcgctgtg tgttgcagcc atcgggttta ttgccgtgca gccggtgttc tggacgatgc
      541 cgacacaact gctttccggt acggcgctgg ctgcggggat tggttttgta aacctgcttg
      601 gtgcagtggg cgggtttatt gccccgatcc tgcgcgtgaa agcagaaacg ttatttgcca
      661 gcgatgcggc gggattactg acgctggcag cggtggcggt catcggttcg ctgattattt
      721 tcactctgcg tgtaaatcgc accgttgcgc agaccgacgt ggcacatcat taaataggtt
      781 aaggaacacg atgaacgcat tattaaccat ccctttaaag aacgtttacg caagggcgaa
      841 gtgcaaattg gtctgtggtt aagctcaacg actgcctata tggcagaaat tgccgctact
      901 tccggttatg actggttgct gattgacggg gagcatgcgc caaacaccat tcaggatctt
      961 tatcaccagc tacaggcggt agccccctat gccagccaac ccgtgatccg tccggtggaa
     1021 ggcagtaaac cgctgattaa acaagtcctg gatattggcg cgcaaactct actgatcccg
     1081 atggtcgata ctgccgatca ggcacgtcag gtggtgtctg ccacgcgcta tcctccctac
     1141 ggtgaacgtg gtgttggggc cagtgtagca cgggctgcgc gctggggacg cattgagaat
     1201 tacatggcgc aagtcaacga ttcgctttgt ctgttagtgc aggtggaaag taaaacggca
     1261 ctggataacc tggacgagat cctcgacgtc gaagggattg atggcgtgtt tattggacct
     1321 gcggatcttt ctgcgtcgtt gggctacccg gataacgccg ggcacccgga agtgcagcga
     1381 attattgaaa ccagcattcg gcggatccgt gctgcgggta aagcggctgg ttttctggct
     1441 gtggctcctg atatggcgca gcaatgcctg gcgtggggag cgaactttgt cgctgttggc
     1501 gttgacacga tgctctacag cgatgccctg gatcaacgac tggcgatgtt taaatcaggc
     1561 aaaaatgggc cacgcataaa aggtagttat tgatatcaaa ggcccatggg gatcggctgt
     1621 gggactgtgt taattagtgg ttattcgctg ccagatcggc ttcgcttagc tgggtggccg
     1681 cgagcaccag gtcacgatca aagccctgtt ccagcatgcg caaggcaatt ttaatagctt
     1741 gttcatgcat gccttcctgc aaaccttcta atttaccttc ctgccaacca atttgatgtc
     1801 cttcctgccg tagccgttca gcaatagtca ttaatctctc cttttgtagt ggtgaacgtt
     1861 cggcaatctc ctgaataaaa cgggtgaaac gggaggtatc gccgcattgc agcagataat
     1921 taaacagtgt ttgtagctgg ctgtcattag tgaagcctct aaccaaaagc gtggtgatcc
     1981 tgtcgaccat accgattaaa tcgcggtcgc gaatatgctt ttgaatcagt tcaagcagag
     2041 ctatacgccg atgttgcatg atctcatcgt caggtacgat ggtaatatcc accaacggaa
     2101 aagcttcggt gtacaactgc cgggcaagtt gcggatcgtc aaactcatcc agccagttga
     2161 gtgagtacgg gtagggcgag gtttcgccat gataaaacag caacggcacc accagcggga
     2221 ctctgtcata gcctttgtcc aggtgacgct gcatggcggc agtggcatag cgcattagcc
     2281 gaaaagccat attcttttct gcagagcttt gatgttcaat cacgcagtag atatagccgt
     2341 caccgtcgct ggtttccacg gaccacaaaa catccgagta gtaagcgcgt aaactttttt
     2401 caataaaact agtgggttcc aggcgtaagg tttgcaggtt gcaaagcttg cgcagtggtt
     2461 ctggtaaatg tatttcgaga aaatcccgtg cggtttcggg tgtgaacata aaggttttaa
     2521 atactgcatc atgcggggag gaggttgttg attcggtcat ggttgtccgt cacctgatat
     2581 ttatgatgac gcgacattac ggttatccca ggcctttgac atctgccagt ttcagccttt
     2641 gcgagacgtt ttcagaggaa ttttcactgc gttgcaacgc atacattggg cacggaatcg
     2701 tgtacgcaaa aatgcccggt aaccacatgc ttaccgggca tcagaaagga gtttaagcca
     2761 gcgcctgggc cagtagcgta atcggatgtt cgcagcgaag acttgtggac atctcaatct
     2821 gccatttaca ggtttcgcag tcggtgacca ccagatctgc gccgctttct tctatctggc
     2881 ggaacagtgg tgcgccgatg gcttgtgagg tggggtagtt ctcttttttg aaaccgtaag
     2941 tacccgcaat accgcagcac tgggaatcca gcaccgttaa ctcaagcccc gggatgttac
     3001 gcaacagctc cagggtgtag agcgtccagc ccattttttc catatggcac ggagtgtgat
     3061 aaaccacttt cagcggcagc ggtttcagcg gtaacgtttt gccttcgtcc agcttgcgcc
     3121 acagccagcg ggtcgccagt tcgatatgat cgcgcaagcc tttgttgtcg acattcagca
     3181 cttccgggta ttcgtcgcgc agggcaaatg tacaggttga ggaggtggca atcaccggaa
     3241 tgccttttac tcccacagct tcgcggatcg actctacatt cgtaattgcc tgtttgcgtg
     3301 ctttatcggt aaagccgttg gcgatcagcg gtacgccgca gcatttttct ttgctgagca
     3361 gttgtacacc ggtacccatt gcgttgagca ctttaattaa atctttacct aactgcggat
     3421 ggttgtagtt aacgaagcag ccgtgaaaga aagcgacctg gtctttatac tgcgcttgct
     3481 gagccgccac gctgcgatac cagcgacgaa acgtgccgaa ggagtatttc ggtagcgtgc
     3541 ggcgatgatc gatttttaac gccgcatcaa gcaactgccg caccggtttc agcgaggtag
     3601 cggtgttgac gattggtgcg aacggcgtgg aaacgctacc catcaggtcg gtatggctta
     3661 aaacaaaatt gcgcagcgac gggcgcgtgg tgtcatattt cgcccgtgcg cgctggatga
     3721 tatcgccaat cttcacatcg gacgggcagg cgacttcgca acgtttgcag ttgatgcaat
     3781 atttcagtgc ctcgtcatac agtgcgccat cttttaaacg cagacgctcg ccatccggcc
     3841 cggcttgttt tggccctgga taaccgggat tcacccggct cactgggcag gcggtggtgc
     3901 agacggtgca cttaatgcag ttttcgaagc tggtgtcatt cattgttggc ctcctgcgcg
     3961 ttgggcaatc tgttgagcgg catgtaaagc actgacggca caaacaccgc cgccgcatcc
     4021 ctgggcgatg ggatcaaatc cgcccagcac cgaaccgatg gtaaacaggt tttcgatggt
     4081 ttgccctgcc tgcgacgggc gtagcgtctg atcagtggtt acaccgaact gctgccacgg
     4141 ttgcggcgca aaaaaatctc ccttatacca ttcaccccgc gtggcggttt gtagcacatc
     4201 aaggccgaga atcggctctc gaatgccgtt acgttccgct accagtccgc cactaaagaa
     4261 actgccgctg gcgagaaccg cgaaacgtgg acgcagcggg atatcggcgt gattgcgggt
     4321 ccagatttca ttcactacgc catttttaca ggtcactttt ttcacttcat cgcccggcat
     4381 ccacacgcca ccctgacgca caaactggcg ctgtaactgg ttttgcagac gaatgcccag
     4441 cacggaaggc ggcagcgttg gcaaaagcat cagtgaacaa ggtagttttt cattcaacca
     4501 acgccacagt ttgtcatcag ccaggccgaa gcaggcgggc atcaggatca tttcgcaggt
     4561 attggcgaca ggaataagcg catcaagtaa cagcggccag ttttcttcat tatcaaggaa
     4621 acgggcgata ttcaccgcgc ggaactcggt ggcgttattg cgcaggacat ccagttccgg
     4681 cagctctatt tctgcggttt caacggcaag gtcgagttca cgcaacgaag ctgccgcaag
     4741 gtgcgcctga aaatccatca gtccgctaat tcccactaca catattttct tcgcgggcag
     4801 cggccagacg gggacttctg gcgaacttag ccaggtagag cgcagagtgc cgagcggcgt
     4861 aatccgctgg tgaggcagtt ctacgctgcc ctgcaattgc gcaccgcttt cagcgattaa
     4921 tgcctgcgcc tggcaagcga gatcgagcac gcgttgtggc ccgagaagag agtaaggatg
     4981 tgcaggtgcc tgctgacgca aagattccag tccactgtga atgtctgtca cagcttgacc
     5041 atctggcaga tggctcagca aatccagcga accggatgag aaatgcagcg cgctttgacc
     5101 acgcgtgaca atggcacagc gcaggccgtg tttttgcagc tgcaggccac agagtaatcc
     5161 ggcgaggccg ccgcccataa tgatagtatc aaagcgcatc tttctgctcc ttctccagac
     5221 cacacagtcc ctgataaacc cagcgggtaa attcgctttc gcgcagtgca tctccccagg
     5281 cgatgggttg cacgcctttc cagcgttcgt taaggaaggt ggaaagttgc tcgatagatt
     5341 gcgcggacgt cgtgacgtta aaacgttgca gcagtccagc agcgcggcag gcgcagagtt
     5401 cgccctggca ggtacccatc cccacacggg tacgacggcg taaatccagc aggctattaa
     5461 cgtttaaatt ttctaccgcg tactgcactt cacccgcagt taccgcttcg cactcacata
     5521 ccaggctacg gtgcagacgg ccttcgctca gccaggcagg cgtgcgatcg ccatgacgat
     5581 aaaccgcaga accgcgcagc ggggcaggca gggagatgac tttacgcaag gtaacttcag
     5641 cgggttcctg tgaacccgga agtgccagat cggcggtcgt acaggggcgc gtgttgccca
     5701 gtttgcggca taccgcgtcg gtagcccatt cagccatcag ccgataggtc atcagtttgc
     5761 caccggtgat ggtgataaat ccgtccagac catcgcgttc agcatggtcg agcagcacga
     5821 tgccacggct gacgttacgt ccgctcgggt cgtcatcgct ggcaaccagc gggcgcacgc
     5881 cagaataggc ccgcaaaatg cgcgttttcg ccatcacggg ggccagtttt tccccttcac
     5941 gcagcagaat atcaacctct tctgccgtca ctcgattatc gtcaatctcg ttgtaatcaa
     6001 tacgtaaaga ggtggtgcca atcagcgaaa tggtatcgcc aggcaccaga atatcggcgt
     6061 cggaaggttt acggcagcgg ttgatcacat gctgattaat gcggtgatcc atgatcagca
     6121 gcgatccttt cgccgggaac atgcgaatgc gcagatcggc atattcggca atgtgttgcc
     6181 cccagatccc agcggcatta accacgacag gtgcatgaag ggcctgagtt tcgccggtga
     6241 gatggttacg tacacgaaca ccgcacaccg tcgcgccttc acgaatcagc cccgtgactt
     6301 catgagcggt aaggataaca gcaccgtgtt ctttggcatc cagcatgttt gctgcggtca
     6361 gacgaaatgg atcaacggtg ccatccggaa ctttcaccgc gccaatcagt gccgggttaa
     6421 cggcaggttc gataatgcgc gcttgctgcg ggtctatagc ttctgcgctg atccctgctt
     6481 cttcgcaggc gcgaataaaa gtggcctgga aggagaggtc atcttccggc agggtgataa
     6541 acaggccgtt ggttggttca acgcagtgac gtgcaatgcg tttcaggatc tggttttcac
     6601 taatgcattc gcgggccgat tccgcatcgg ttaccgcata gcgcgcaccg ctgtgcagca
     6661 ggccgtggtt acgcccggtg gcaccggttg cgatgtcgtg gcgctcaacc aaaatcacgc
     6721 gcagcccgcg cagggcacag tcgcgggcaa ttccggctcc cgttgcgccg ccgccaatga
     6781 taatcacgtc acttgattgc gagtcgcgag ttttcattgt tatccctctg aagttcgttt
     6841 tttaccattt agccatagta aaaacatgag ttgtttgatt tcgcgcatat tcactcataa
     6901 ttcgaaagtg agacgtgatt tcatgcgtca ttttgaacat tttgtaaatc ttatttaata
     6961 atgtgtgcgg caattcacat ttaatttatg aatgttttct taacatcgcg gcaactcaag
     7021 aaacggcagg ttctctcact gaatcaggct gttaatcata aataagacca cgggccacgg
     7081 aggctttcaa tgttgagtat ttttaaacca gcgccacaca aagcgcgctt acctgccgcg
     7141 gagatcgatc cgacttatcg tcgattgcgc tggcaaattt tcctggggat attctttggc
     7201 tatgctgctt actatttggt tcgtaagaac tttgcgctcg ctatgcctta cctggtggag
     7261 cagggattct cacgcggtga tttaggtttt gcgctttctg ggatctcgat cgcctatgga
     7321 ttttcgaaat tcatcatggg ttcggtatcg gatcgctcga atccgcgcgt tttcctgccc
     7381 gcaggtttga ttctggcggc ggcagtgatg ttgtttatgg gctttgtgcc atgggcgacg
     7441 tcgagcattg cggtgatgtt tgtactgttg ttcctctgcg gttggttcca ggggatgggg
     7501 tggccgccat gcggtcgtac tatggtgcac tggtggtcgc agaaagaacg tggcggcatt
     7561 gtgtcagtgt ggaactgtgc gcacaacgtc ggtggtggta ttccgccgct gctgttcctg
     7621 ctggggatgg cctggttcaa tgactggcac gcggcgctct atatgcccgc tttctgcgcc
     7681 attctggtgg cattattcgc ctttgcgatg atgcgcgata ccccgcaatc ctgtggcttg
     7741 ccgccgatcg aagagtacaa aaatgattat ccggacgact ataacgaaaa agcggaacag
     7801 gagctgaccg cgaagcaaat cttcatgcag tacgtgctgc cgaacaaact gttgtggtat
     7861 atcgccatcg ccaacgtgtt cgtttatctg ctgcgttacg gcatcctcga ctggtcaccg
     7921 acttatctga aagaggttaa gcatttcgcg ctggataaat cctcctgggc ctacttcttc
     7981 tatgagtatg cgggtattcc gggcaccctg ctgtgcggct ggatgtcgga taaagtcttc
     8041 cgtggcaacc gtggggcaac cggcgttttc tttatgacac tggtgaccat cgcgactatc
     8101 gtttactgga tgaacccggc aggtaaccca accgtcgata tgatttgtat gattgttatc
     8161 ggcttcctga tctacggccc tgtgatgctg atcggtctgc atgcgctgga actggcaccg
     8221 aaaaaagcgg caggaacggc agcgggcttt accggtctgt ttggttacct gggcggttcg
     8281 gtggcggcga gcgcgattgt tggctacacc gtggacttct tcggctggga tggcggcttt
     8341 atggtaatga ttggcggcag cattctagcg gttatcttgt tgattgttgt gatgattggc
     8401 gaaaaacgtc gccatcagga attactgcaa aaacgcaacg gaggctaatg gcatgaaatt
     8461 gacgctgaaa aaccttagca tggcgatcat gatgagcact atagtcatgg gaagcagtgc
     8521 aatggcggcg gacagcaacg aaaaaatagt catcgcccat cgcggtgcca gtggatattt
     8581 gccggagcat acgctgccag caaaagcgat ggcgtatgcg cagggagcgg attatctgga
     8641 acaggatttg gtgatgacca aagacgacca tctggttgtt ctgcatgacc attacctcga
     8701 tcgtgttact gatgttgccg atcgtttccc ggatcgggcg cgcaaagacg gtcgttacta
     8761 cgcgatagat ttcacgctgg atgaaattaa gtcgttgaaa tttaccgaag gtttcgatat
     8821 tgaaaacggt aaaaaagtgc agacttatcc agggcgtttc ccaatgggta agtccgactt
     8881 ccgggtgcac acctttgaag aagagattga atttgttcag gggttaaatc actctaccgg
     8941 gaaaaatatc ggtatctatc cagaaatcaa agcgccgtgg ttccatcatc aggaagggaa
     9001 ggatattgcg gcaaaaacgc tggaagtgct gaagaaatat ggttacaccg gtaaagacga
     9061 taaagtttat ttgcaatgtt ttgatgctga tgagctgaag cgtattaaga atgagctgga
     9121 acccaaaatg ggcatggatc tcaatctggt acagctgatt gcctataccg actggaatga
     9181 aacgcagcag aaacagccgg acggaagctg ggttaattac aactacgact ggatgtttaa
     9241 gccgggtgcc atgaaacagg tggcggaata tgcagatggt attggtccgg attaccatat
     9301 gttgattgag gagacatcgc agccgggtaa tatcaaactc actggcatgg tgcaagatgc
     9361 tcagcagaac aagctggtag tgcatcctta taccgtgcgg tcagataaac tgcctgaata
     9421 cacaactgat gtgaatcagt tatatgatgc tctgtataac aaagcgggtg taaatgggtt
     9481 gtttactgat ttccctgata aagcggttaa attccttaat aaagagtaat gctgctactg
     9541 caggatggcg ttcatgcgcc atcctctgct aaagcgaaat aatgctacta ccggttattc
     9601 ttttcattaa ccggtgcgtc ctgtaaaaaa gatgccaaca taattttcgt aaaactcatt
     9661 cgttaaaaaa gctaacactg ggcattgtta ttctttaaac agtatgaata ggtctcaata
     9721 at
//
LOCUS       DBFIFM010000170         9566 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.170, whole genome shotgun sequence.
ACCESSION   DBFIFM010000170 DBFIFM010000000
VERSION     DBFIFM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.170"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            196..1065
                     /gene="hexR"
                     /locus_tag="N7C74_003682"
                     /gene_synonym="yebK"
     CDS             196..1065
                     /gene="hexR"
                     /locus_tag="N7C74_003682"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HGU2433476.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            1193..2635
                     /gene="pyk"
                     /locus_tag="N7C74_003683"
     CDS             1193..2635
                     /gene="pyk"
                     /locus_tag="N7C74_003683"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HGU2433477.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGINYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(2766..3737)
                     /gene="lpxM"
                     /locus_tag="N7C74_003684"
                     /gene_synonym="msbB"
     CDS             complement(2766..3737)
                     /gene="lpxM"
                     /locus_tag="N7C74_003684"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310592.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HGU2433478.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRIAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(3857..5179)
                     /gene="mepM"
                     /locus_tag="N7C74_003685"
     CDS             complement(3857..5179)
                     /gene="mepM"
                     /locus_tag="N7C74_003685"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HGU2433479.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(5195..6127)
                     /gene="znuA"
                     /locus_tag="N7C74_003686"
     CDS             complement(5195..6127)
                     /gene="znuA"
                     /locus_tag="N7C74_003686"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HGU2433480.1"
                     /translation="MLHKKTLLFAALFAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSINGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            6206..6961
                     /gene="znuC"
                     /locus_tag="N7C74_003687"
     CDS             6206..6961
                     /gene="znuC"
                     /locus_tag="N7C74_003687"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HGU2433481.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            6958..7743
                     /gene="znuB"
                     /locus_tag="N7C74_003688"
     CDS             6958..7743
                     /gene="znuB"
                     /locus_tag="N7C74_003688"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HGU2433482.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTDGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7989..8999)
                     /gene="ruvB"
                     /locus_tag="N7C74_003689"
     CDS             complement(7989..8999)
                     /gene="ruvB"
                     /locus_tag="N7C74_003689"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HGU2433483.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(9008..9565)
                     /gene="ruvA"
                     /locus_tag="N7C74_003690"
     CDS             complement(9008..9565)
                     /gene="ruvA"
                     /locus_tag="N7C74_003690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HGU2433484.1"
                     /translation="MEVGGVGYEVHMPMTCFYELPEAGQEAIVFTHFVVREDAQLLYG
                     FNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAVEREEVGALVKLPGIGKKT
                     AERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAEQEAVAALVALGYKPQEAS
                     RMVSKIARPDASSETLIREALRAAL"
BASE COUNT         2264 a         2460 c         2401 g         2441 t
ORIGIN      
        1 gtgcggcaaa actgataaaa aaatcatcgt tttccattgg gtaaaaatct gacactgatc
       61 atgttatgaa aaaaaataac aactttttta tctgcttttg tcattaacac agcacacagg
      121 cgtaatatct gacaaaactg catttcgatt tctttcagtg cggaaatcgt cattacccgt
      181 gagtctcttt acatcatgaa tatgctggaa aaaatccagt ctcagctgga acatttgagc
      241 aaatcagagc gcaaagttgc cgaggtcatt ctggcttcgc ccgataacgc gatccattcg
      301 agtattgctg ctctggcact ggaagccaat gttagcgaac cgacggtgaa tcgtttctgt
      361 cgcagcatgg acacgcgcgg ttttcctgat tttaaacttc atctggcaca gagtctggcg
      421 aatggcactc cctatgttaa tcgcaacgtc aatgaagatg acagcgttga atcatacaca
      481 gggaaaatat ttgagtccgc aatggcaacg ctcgatcatg tccgtcattc actggataaa
      541 tctgccatca accgcgccgt cgacttgctc actcaggcaa aaaaaatcgc ctttttcgga
      601 ttaggctctt cagccgccgt tgcccacgat gcgatgaata agttctttcg ttttaatgtt
      661 ccggtggtgt actccgatga tattgtgctg caacgcatga gttgtatgaa ttgtagcgac
      721 ggagacgtgg tggtgctgat ttctcacacc ggaagaacaa aaaatctggt cgagctggcg
      781 cagctggcac gcgaaaacga cgctatggtg attgccctca cctctgcggg taccccgctc
      841 gcccgggaag caacgctggc aattaccctc gacgtaccgg aagatactga catttatatg
      901 cccatggttt ctcgacttgc acagctgacc gtgatagatg tgctggcgac aggatttact
      961 ttgcgacgcg gtgcaaaatt cagagataac ttgaagcggg tcaaggaagc gctgaaggaa
     1021 tcgcgttttg ataagcagtt acttaattta agtgacgatc gctaaaagcg actgtcactg
     1081 ttctaatctt atacgacatc cgaatgagat tattttatcg ccatcgcggc gttatttcat
     1141 tcggatttca tgttcaagca acacctggtt gtttcagtca acggagtatt acatgtccag
     1201 aaggcttcgc agaacaaaaa tcgttaccac gttaggccca gcaacagatc gcgataataa
     1261 tcttgaaaaa gttatcgcgg cgggtgccaa cgttgtacgt atgaactttt ctcacggctc
     1321 gcctgaagat cacaaaatgc gcgcggataa agttcgtgag attgccgcaa aactggggcg
     1381 tcatgtggct attctgggtg acctccaggg gcccaaaatc cgtgtatcca cctttaaaga
     1441 aggcaaagtt ttcctcaata ttggggataa attcctgctc gacgccaacc tgggtaaagg
     1501 tgaaggcgac aaagaaaaag tcggtatcaa ctacaaaggc ctgcctgctg acgtcgtgcc
     1561 tggtgacatc ctgctgctgg acgatggtcg cgtccagtta aaagtactgg aagttcaggg
     1621 catgaaagtg ttcaccgaag tcaccgtcgg tggtcccctc tccaacaata aaggtatcaa
     1681 caaacttggc ggcggtttgt cggctgaagc gctgaccgaa aaagacaaag cagacattaa
     1741 gactgcggcg ttgattggcg tagattacct ggctgtctcc ttcccacgct gtggcgaaga
     1801 tctgaactat gcccgtcgcc tggcacgcga tgcaggatgt gatgcgaaaa ttgttgccaa
     1861 ggttgaacgt gcggaagccg tttgcagcca ggatgcaatg gatgacatca tcctcgcctc
     1921 tgacgtggta atggttgcac gtggcgacct cggtgtggaa attggcgacc cggaactggt
     1981 cggcattcag aaagcgttga tccgtcgtgc gcgtcagcta aaccgagcgg taatcacggc
     2041 gacccagatg atggagtcaa tgattactaa cccgatgccg acgcgtgcag aagtcatgga
     2101 cgtagcaaac gccgttctgg atggtactga cgctgtgatg ctgtctgcag aaactgccgc
     2161 tgggcagtat ccgtcagaaa ctgttgcagc catggcgcgc gtttgcctgg gtgcggaaaa
     2221 aatcccgagc atcaacgttt ctaaacaccg tctggacgtt cagttcgaca atgtggaaga
     2281 agctattgcc atgtcagcaa tgtacgcagc taaccacctg aaaggcgtta cggcgatcat
     2341 taccatgacc gaatcgggtc gtaccgcgct gatgacctcc cgtatcagct ctggtctacc
     2401 aattttcgcc atgtcgcgcc atgaacgtac gctgaacctg actgctctct atcgtggcgt
     2461 tacgccggtg cactttgata gcgctaatga cggcgtagca gctgccagcg aagcggttaa
     2521 tctgctgcgc gataaaggtt acttgatgtc tggtgacctg gtgattgtca cccagggcga
     2581 cgtgatgagt accgtgggtt ctactaatac cacgcgtatt ttaacggtag agtaagtacg
     2641 ttgccggatg cggcgaaaac gccacatccg gcctacagtt caatgatagt tcaacagatt
     2701 tcgaatattc tgaagcaaac ttgaacttat catcaggcga aggcctctcc tcgcgagagg
     2761 cttttttatt tgatgggata aagatctttg cgcttatacg gctggatttc gcccggtttg
     2821 cgagttttca gcaattttag tatccaggtg tattgttctg gtcgcggacc aacaaaaatc
     2881 tcgacttctt cattcatccg ccgcgcaatc gtatgatcat ccgcctctaa cagatcatcc
     2941 atcggtgggc gcacctgaat cgtcagacga tgcgtcttgc catcataaat cggaaacagc
     3001 ggtacaacgc gcgcacggca cactttcatc aaacgaccaa tcgcgggcaa cgtcgcttta
     3061 taggtggcaa agaaatccac aaattcgctg tgctctgggc catgatcctg atcgggtaaa
     3121 taatatcccc agtacccctg acgtaccgac tggatgaatg gtttaatacc gtcatttctc
     3181 gcatgcagac gaccgccaaa gcgacgacgc accgtgttcc agacataatc aaaaaccggg
     3241 ttgccctgat tatggaacat cgctgccatt ttctgccctt gcgaggccat cagcatggca
     3301 ggaatatcga cggcccaacc gtgcggcact agaaagataa ctttctcgtt attacgccgc
     3361 atctcttcga tgatctccag cccttgccag tcaacgcgcg gctgaatttt ctccggcccg
     3421 cgtattgcca actcagccat cattgccatc gcttgcggcg cggtggcaaa catctcatca
     3481 acaatcgctt cgcgttcagc ttcactacgt tctggaaagc agagcgacag attgattaac
     3541 gcacgacggc gtgagctttt tcccagtcgt ccggcaatac gtcccagccg tgccagaatg
     3601 ggatcacgga actttggcgg cgttaaagcg atacccgcca tcgctgctac gcccagccat
     3661 gctccccagt agcgcgggtg gcgaaaggat ttatcaaact caggaatgta ttcgctatta
     3721 ttttttttcg tttccatgct tttccagttt cggataaggc aaaaatcaat ctggtgatag
     3781 tgtagcggcg caacttgccc cgcaccaaat aaaaaaagcc ggtactgact gcgtaccggc
     3841 tgcgaatgga tgttaattaa tcaaaccgta gctgcggcac aatctctttg gcctgcgcca
     3901 ggaattcgcg acgatcggag ccggtcagcc cttcggtacg cggcagtttt gccgtcagcg
     3961 ggtttacggc ctgctggttt atccatactt catagtgcag atgcggcccg gttgaacgtc
     4021 cggtattacc ggaaagcgcg atacggtcgc cacgtttcac cttctgcccc ggtttcacca
     4081 gaatcttgcg caagtgcata taacgcgtgg tgtagctgcg accatgacga atagccacat
     4141 aataacctgc tgcgccactg cgtttggcaa ccaccacttc accgtcaccc actgaaagca
     4201 ctggcgtacc ctgcggcatg gcgaaatcaa cacctctgtg tggtgcaacg cgaccggtca
     4261 ccggattagt acgacgcggg ttaaagttag acgagatacg gaactgtttc gccgtcggga
     4321 atcgcaagaa tcctttcgcc agaccagtac cgttacgatc gtagaatttg ccatcttcag
     4381 cgcggatagc gtaataatct ttaccttctg aacgcaaacg tacgcccagc agctggcttt
     4441 gctcacgttt accatcaagc atttctcgag acattaatac cgcaaattca tcgccttttt
     4501 tcagtttgcg gaaatccatt tgccactgca tggctttaat cactgcgctc acttcggcgc
     4561 tggtcaaacc ggcgtttctg gcgctggcaa caaagctccc cccgacggta cctttcagca
     4621 gattgttaac ccactctcct tgctgcattt cgctggtcat tttaaaaccg ttagcggcag
     4681 tacggtcata ggttcgggtt tcacgacgag acacttccca ggtgaggcgc tgcagttcgc
     4741 cgtccgcggt taatgtccag gagagttgtt gaccgatttt caggttacgc aattctttgt
     4801 cggccgcagc cagttgggtg atatcaccca tatcaatacc atactgattg agaatgctgc
     4861 ttagcgtatc gccagtggaa acaacatatt catgcacgcc cgcttcaccg gcgattttgt
     4921 catccagttc gtcctgggga atggcttcat cttcttgtgc agcttgatca atcggctcac
     4981 tggcttcagg taagagcgaa cgaatttcgt tctgttccag ctcaatggtt ttgacaattg
     5041 gcgtggcatc gcggtgataa acatagggcc gccagacagc gacggccaga gtaagaacgg
     5101 tgagcgaccc caacataacg cggtgtggtc gcggtaaatt attaaacgcc agggcgacag
     5161 agcgggctat ctgttgcacg taatcacttc ctcattaatc tcctttcagg cagctcgcat
     5221 actggttggc taattgactc aggaattctg aatagcttgt tttacccagt ttgatattcg
     5281 tccccaaggg atccaacgtt cccatacgaa cggatgtccc tcgtgcgacg ctctcaacga
     5341 ccgctggcct gaactgtggc tcagcaaaaa cgcaggttgc tttttgctca accaactgtg
     5401 ttcttatttc atgtaaacgc tgcgcgccag gttgaatctc agggttaacg gtaaaatgac
     5461 caagcggtgt cagtccgaac tgtttttcga aatagccgta agcatcgtga aaaacgaaat
     5521 aacctttccc cttgagcggc gcgagctcgt taccaacctg cgtttcggtt gaggctaatt
     5581 gtgcctcaaa atccttcagg ttggcgtcaa gtttggctcg actttgcggc ataagttcca
     5641 ctaattttcc atggattgca accgctgtag cccgcgctat ctctggggaa agccaaagat
     5701 gcatgttgaa atcgccgtga tggtgatctt cgtcactttt ttccgcgtgg tcgtgatcat
     5761 catcatcgcc gttaatactt ttcatcagta gcggtttcac atcttcaagc tgcgcaatcg
     5821 ttacctgctt cgcttctggt aatttgctta ccggtttttg cataaacgct tccatctccg
     5881 ggccaaccca aacgactaag tccgcgttct gtaagcgttt tacatccgat ggacgcagtg
     5941 aataatcatg ttctgaagcg ccgtcaggaa gtaaaacctc cgtttctgtt accccatcag
     6001 caatggcaga agcgatgaac ccaacgggtt taagcgaagc gacaacggcg gcatctgcgg
     6061 cctgtgttgc accgccccag agagcggcga ataatgctgc gaaaagaagc gtttttttat
     6121 gtaacataat gcgaccaatc atcgtaatga atatgagaag tgtgatatta taacatttca
     6181 tgactactgc aagactaaaa ttaacatgac aagtctggtt tccctggaaa atgtctcggt
     6241 ttcttttggc caacgccgcg tcctctctga tgtgtcgctg gaacttaaac ctggaaaaat
     6301 tttgacttta cttgggccaa atggcgcagg taagtcgaca ctggtacggg tagtgctcgg
     6361 gctggtaaca cccgatgaag gggttatcaa gcgcaacgga aaactgcgca tcggctatgt
     6421 accacagaag ctgtatctcg acaccacgtt gccactgacc gtaaaccgtt ttttacgctt
     6481 acgccctggt acacataaag aagatatttt gcctgcactg aaacgtgtcc aggccgggca
     6541 cctgattaac gcaccgatgc aaaagctctc tggtggcgaa acgcagcgtg tactgttagc
     6601 gcgagcattg ttaaatcgcc cgcaattatt agtgctggat gaacccactc aaggcgtgga
     6661 tgtaaatggc caggtggcgt tatatgacct tattgaccaa ctgcgtcgtg aactggattg
     6721 tggcgtttta atggtttctc acgatcttca tctggtgatg gcaaaaaccg atgaagtgct
     6781 gtgcctgaat catcacattt gttgttccgg cacaccggaa gttgtttccc tgcatccgga
     6841 gtttatttca atgtttggtc ctcgtggtgc tgaacaactg ggtatctatc gccatcatca
     6901 taatcatcgt cacgatttac agggacgaat tgttttgcgt cggggaaatg atcgctcatg
     6961 attgaattat tatttcccgg ttggttagcc gggatcatgc tcgcctgtgc cgcgggtccg
     7021 ctgggttcgt ttgtagtctg gcgtcgtatg tcttatttcg gtgatacgct ggctcatgcc
     7081 tcattacttg gtgtcgcgtt tggtttgttg ctggacgtga atccattcta tgcggtgatt
     7141 gccgttacgc tgctgctggc gggcggtctg gtatggctgg agaagcgtcc acagctggcg
     7201 atcgacacgt tattagggat tatggcgcac agtgccctgt cgctgggcct ggtggtcgtt
     7261 agtctgatgt ctaacattcg tgttgatttg atggcttacc tgttcggtga tttactggca
     7321 gtgacgccag aagatctcat ctctattgca attggcgtgg tcatcgtggt ggctattttg
     7381 ttctggcaat ggcgcaattt gctgtcgatg acgattagcc cggatctggc gtttgttgat
     7441 ggtgtgaaat tacagcgagt gaaattgttg ttgatgctgg tgacggcatt gacgattggt
     7501 gtagcgatga aatttgtcgg tgcgttgatt attacttcgt tgctgattat tcctgcagct
     7561 actgcgcgtc gctttgcccg tacgccggaa cagatggctg gtgtcgctgt tttggtgggg
     7621 atggtggcag taactgacgg tttaaccttt tccgcgtttt acgatacgcc ggcgggtccg
     7681 tcggtggtgc tatgtgcggc actgttattt attatcagta tgatgaaaaa gcaggccagc
     7741 taatctatcg ctgaacacat ttgtcggatg cggcgcgagc gccttatccg acctacggtt
     7801 cgctatctct ggtaggcctg gtaagacgcg aacagcgtcg catcaggcac actgccaatg
     7861 tcggatgcgg cgcgagcgcc ttttccgacc tacggttcgc tatctctggt atatgcctga
     7921 taagacgcga atagcgtcgc atcaggcaca ctgccaatgt cggatgcggt gcgagcgacc
     7981 aatccgactt acggcatttc tggcggcgtt atgccaaagt gattccacgc ccgcgtcgtc
     8041 gccatacgcc cacgcggtgt acgctgcaaa aagccttgct gaatcaaata aggttccagc
     8101 acatcctcaa tggtttcacg ttcttcgcca atggctgccg caaggttatc cagacctacc
     8161 ggcccaccaa agaacttatc gattaccgcc agcaacaatt tgcggtccat ataatcgaaa
     8221 ccttcagcat cgacattcaa catatccagc gcctgagcag cgatatctgc cgagatggtg
     8281 ccatcgtgct tcacttcggc gaaatcacgc actcgacgca gcagacggtt ggcaatgcgc
     8341 ggcgtaccgc gagcgcgacg agcaacttcc agcgcgccgt catcactcat ctcaagcccc
     8401 ataaagcgtg cgctgcgact gacgatatat tgcagatccg gcacctgata aaactccaga
     8461 cgttgcacaa taccaaaacg gtcgcgcaac ggtgatgtca gcgaacctgc gcgcgtggtt
     8521 gcaccaatca gggtaaacgg cggcaaatca attttaatgg agcgtgccgc cggaccttca
     8581 ccaatcatga tatccagttg gtagtcttcc attgccgggt acagcacttc ttcaacaact
     8641 ggcgataaac ggtggatctc atcaataaac agcacgtcat gcggttcaag gttagtgagc
     8701 atcgcagcca aatcgcccgc cttttccagc accggaccag aagtcgtgcg taaattaacg
     8761 cccatttcat tggcgacaat gttggcaagc gtagttttac ccaaccccgg aggaccaaaa
     8821 atcaacaaat gatcgagggc atcgccgcgc agtttcgctg ctttgatgaa aatctccatc
     8881 tgtgaacgaa cctgcggctg accaacatac tcttccagta atttagggcg aatggcgcga
     8941 tctgctacat cttccggcaa agtggtaccg gcagaaatca gacggtctgc ttcaatcatc
     9001 ctttacctca taacgcggcg cgtagggctt cgcgaattaa tgtttcactg ctggcgtcag
     9061 ggcgagcgat tttgctcacc atgcggcttg cttcttgtgg tttatagccc agcgccacca
     9121 gcgcggcaac cgcttcttgt tcagcatcgt cggtcgccgg gctggcagga gacgtgagta
     9181 ccaggtcggc ggctggcgta aagagatcgc catgcaaacc tttaaatcgg tctttcattt
     9241 caacaatcaa gcgttcggcg gtttttttgc caatacccgg cagtttcacc agtgccccca
     9301 cttcttcacg ctcaacggca ttaacgaact gctgcgctga cattccggag aggatcgcca
     9361 gcgccaactt cgggccgacg ccgttggttt tgatcaactc tttgaacaac gtgcgctctt
     9421 gtttattgtt aaaaccgtac agcagttgcg cgtcttcacg caccacaaag tgggtgaaaa
     9481 cgatcgcttc ctgacccgct tcagggagtt cataaaaaca ggtcatcggc atatgcactt
     9541 catagcctac gccgcccact tcaatt
//
LOCUS       DBFIFM010000171         9506 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.171, whole genome shotgun sequence.
ACCESSION   DBFIFM010000171 DBFIFM010000000
VERSION     DBFIFM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.171"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            1..1296
                     /gene="dcuC"
                     /locus_tag="N7C74_003691"
                     /pseudo
     CDS             1..1296
                     /gene="dcuC"
                     /locus_tag="N7C74_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
     gene            complement(1339..1836)
                     /gene="sspB"
                     /locus_tag="N7C74_003692"
     CDS             complement(1339..1836)
                     /gene="sspB"
                     /locus_tag="N7C74_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HGU2433485.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(1842..2480)
                     /gene="sspA"
                     /locus_tag="N7C74_003693"
     CDS             complement(1842..2480)
                     /gene="sspA"
                     /locus_tag="N7C74_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HGU2433486.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(2875..3267)
                     /gene="rpsI"
                     /locus_tag="N7C74_003694"
     CDS             complement(2875..3267)
                     /gene="rpsI"
                     /locus_tag="N7C74_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HGU2433487.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3283..3711)
                     /gene="rplM"
                     /locus_tag="N7C74_003695"
     CDS             complement(3283..3711)
                     /gene="rplM"
                     /locus_tag="N7C74_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HGU2433488.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(3930..5057)
                     /gene="zapE"
                     /locus_tag="N7C74_003696"
     CDS             complement(3930..5057)
                     /gene="zapE"
                     /locus_tag="N7C74_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HGU2433489.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5251..5649
                     /gene="zapG"
                     /locus_tag="N7C74_003697"
     CDS             5251..5649
                     /gene="zapG"
                     /locus_tag="N7C74_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HGU2433490.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            5803..7170
                     /gene="degQ"
                     /locus_tag="N7C74_003698"
     CDS             5803..7170
                     /gene="degQ"
                     /locus_tag="N7C74_003698"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HGU2433491.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKTGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7260..8327
                     /gene="degS"
                     /locus_tag="N7C74_003699"
     CDS             7260..8327
                     /gene="degS"
                     /locus_tag="N7C74_003699"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HGU2433492.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8391..9329)
                     /gene="mdh"
                     /locus_tag="N7C74_003700"
     CDS             complement(8391..9329)
                     /gene="mdh"
                     /locus_tag="N7C74_003700"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HGU2433493.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT         2297 a         2441 c         2423 g         2345 t
ORIGIN      
        1 actacaaacc tcaggtggtg ctggctgcag gtatcttcct gatgatgtgc ggtgtctggt
       61 tagggttagg tggtgtactc gatcccgcca aaagcagcgg ctacttgatc gtcgatattt
      121 ataatgaaat cctgcgcatg ctgtccaacc gcattgccgg attggggctg tcgattatgg
      181 cggtgggcgg ttatgcccgc tacatggagc gcacaggagc cagtcgcgcg atggtgagct
      241 tgttaagccg cccgttaaaa ctcattcgct cgccgtatat tattctgtcg gcaacttacg
      301 tcatcggcca aatcatggcg cagtttatta ccagcgcctc cggcctgggt atgttgctga
      361 tggtcacctt atttccgaca ctggtgagtc tgggagtaag ccgtctctct gcggtggcgg
      421 ttatcgcaac cacaatgtcc attgagtggg ggattctgga aacgaactcc atttttgcag
      481 cccaggtagc gggaatgaaa attgccactt acttcttcca ctaccagctt ccggtcgcct
      541 cttgcgtcat tatctcggtg gcgatctccc actttttcgt gcaacgcgct tttgacaaaa
      601 aagataaaaa tatcaatcac gaacaggcag agcaaaaagc tctcgataat gtcccgccgc
      661 tctattacgc cattttacct gtgatgccgt taatcctgat gctcggctcg ctattccttg
      721 cccacatcgg gctgatgcag tcagaactgc atctggtggt ggtgatgtta ctgagtttga
      781 cgatgtttgt tgagttcttc cgcaagcata acttgcgcga aacaatggac gatgtgcagg
      841 cgttttttga cggcatgggt acgcagtttg ccaacgtggt aacgctggtg gtcgcgggtg
      901 aaatatttgc gaaaggctta acgacgattg gcactgtcga tgcggttatc aggggtgcgg
      961 agcattctgg tctgggcggt attggcgtga tgattattat ggcgctggtc attgccattt
     1021 gtgccattgt gatgggctct ggcaatgcgc cgtttatgtc atttgccagt cttattccga
     1081 atatcgcagc cggactacat gtaccagcgg ttgtaatgat tatgccgatg cattttgcca
     1141 cgacgctagc gcgcggtttc gccgattact gcggtggtgg tcgttacgtc aggaattgca
     1201 ggcgtttcgc cttttgcggt ggtgaagcgg acagcgatcc ccatggcagt cggtttcgtg
     1261 gtgaatatga ttgccacaat cacgctattt tattaagtca ttaaaagaca aaacaggccg
     1321 ccagggcctg tttttgtatt acttcacaac gcgtaatgcc ggtcgaccac cgcgtggtgg
     1381 ctgcggtggt tcatcgtcag gatgagtgtc atcatcgtga tctggcttgt cgccatcaat
     1441 aaccgacata acggtttcgt tgtctgccga tgcctcttca tcattcatga tgctggtatc
     1501 ttcatcgtag gcagcttcag gctcaaacat cgtgcctgcg ccattttcac gggcgtagat
     1561 agccagcacg gcagccagcg gcacagaaac ctgacgcgga atgccaccaa agcgcgcgtt
     1621 aaagcgcacc tcatcattcg ccagttccag attgccgaca gcacgcggcg caatgttgag
     1681 tacgatttgc ccgtcacgcg catattccat aggaacctgc acgccaggga gcgtcacatc
     1741 caccaccagg tgcggcgtga gctggttatc cagcaaccac tcatagaatg cacgcagcag
     1801 atagggacga cgtggtgtta gctgtgacaa atccatacag attaactccg gcccagacgc
     1861 atttcacgtt ctgcttcagt taaagaagca aggaaagagt cacgctcaaa gacgcgggtc
     1921 atatagcctt tcagctcttt cgcacccggg ccgctgaact cgatacccag ttgcggcaga
     1981 cgccacagca gcggagcaag gtagcaatcg accaggctga actcatcgct caggaagtac
     2041 ggcttctgac cgaagaccgg cgcaatcgcc agcagttctt cgcgcagttg cttacgtgcg
     2101 gcatctgctt cagaagctga accgttgatg atggtgttca tcagcgtgta ccagtctttt
     2161 tcgatgcgat gcatgtacaa acggctttca ccgcgagcta ccgggtaaac aggcatcagt
     2221 ggcggatgcg ggaaacgctc atccagatat tccataatga tgcgagattc ccacagggtc
     2281 agctcacgat ccaccagggt cggaacgctc tgattcgggt tgaggtcaat cagatcctga
     2341 ggcggattgt ccttttccac gtgttcgatc tcgaaactta cacctttctc agccagcaca
     2401 atgcggacct gatggctata gatgtcagta ggaccggaaa acagcgtcat taccgaacgt
     2461 ttgttggcag cgacagccat gaaaacctcc aggtatagtc agaattttta ctgctaccag
     2521 ccaccaggtg gccagtcaga agttgtgtta cccagtaagg aacgactctc tttgttcgaa
     2581 aatcaaacaa aaaatgagca atacccgaca tttgggcaga aaattggatg atagtttacc
     2641 agattttgcg acctttgtgg tgagtcgatg ccggaaatgg ggaaaaagag atgcgcttta
     2701 gtctgaaata gttgacttag tcccttattg gcgatgtggt ttttgttttg cctgtctgtc
     2761 agacggcagc aaaaagcaac tttccatttt ttacgctgat tcagaattta accataaaaa
     2821 aacccgccga agcgggtttt ttcgcaaatt gttttctgcc ggagcagaag ccaattaacg
     2881 tttggagaac tgcggacgac gacgtgcttt acgcagaccg actttcttac gttcaacctg
     2941 acgagcgtca cgagtaacga agccagcttt acgcagttca gaacgcaggg actcgtcgta
     3001 ttccatcaga gcgcgggtga taccgtgacg gatcgcacca gcctgaccag agataccacc
     3061 acctttaacg gtgatgtaca ggtccagttt ctcaaccata tcgaccagtt ccagcggctg
     3121 acgaactacc atgcgagcag tttcacgacc gaagtactgt tccagagaac gttggttgat
     3181 tacgatttta ccgttgcccg gtttgatgaa aacgcgagct gcggaacttt tgcggcgacc
     3241 agtgccgtag tattgatttt cagccattgc ctataatccc gattagatgt caagaacttg
     3301 cggttgctgt gccgcgtggt tgtgctcgtt acccgcgtaa actttcagtt tacggaacat
     3361 agcacgaccc agcgggcctt ttggcaacat gcctttaacc gcgatttcaa tcacacgctc
     3421 aggacggcga gcaatcatct cttcaaaggt cgcttgtttg ataccaccga tgtggccggt
     3481 gtggtgatag tacactttgt cagtacgctt gttgccggtt acagcaactt tgtcagcgtt
     3541 cagaacgatg atgtaatcac cggtatctac gtgcggagtg tattccgctt tgtgcttacc
     3601 gcgcaggcga cgagccagtt cagtagccag acggcccaga gttttaccgg tcgcgtcaac
     3661 aacataccag tcgcgtttta cggtttctgg tttagctgta aaagttttca ttaaaagctt
     3721 acccaataaa tagttacacg ttggtgaaca cccaaacgtc ttcaattgtt gaggttcaca
     3781 cgacaaagtc cggcaaacct accccttcga atagcctatg ccagcacaca aaaagttttg
     3841 ggaaaaaaac tttcttgtaa cgtggggtcg caggattata gagaagtcgg ggtcaaagat
     3901 cgaccccttt ttgtgatttg tggcaggttt taacccgcta aatgctcgcg cttcagatac
     3961 tcttcgcttt gcatctcttg cagacgtgac aggcaacgct ggaactcaaa cttcagccga
     4021 tcgccctgat aaatttcata cagcggcact tctgcactca ccactaattt gacatggcgc
     4081 tcgtaaaact catccaccag cgcaataaag cgccgcgctt cgctctccat caaccgcgtc
     4141 ataactggta catcaaacaa catgaccgta tgaaagagac gtgagagcgc aatatagtca
     4201 tgctgactgc gggcgtcgac gcacagcgta gtaaaagaga ccgccagcgt ctggttctcg
     4261 acgcccattg tcgctaatgg ccgatggttg atttctaacg tcggtgaatt ttctcgtttc
     4321 ccccccgcca gcgccaacca tagtttatcc atttgcgccc gggtttcatc gtggagtggc
     4381 gaaagccaca gatgcgcctg agtgagtgta cgcagacgat aatcaacacc agcgtccacg
     4441 ttcattacat cacaatgctg tttaatggca tcgattgcag gcagaaaacg cgcacgttgc
     4501 aagccatttc gataaagttc gtccggcgga atatttgacg tcgctaccag ggtaataccg
     4561 cgggcgaaca gggctttcat caggccgcca agtagcatgg catcggtaat atcagagaca
     4621 aaaaattcgt caaaacagag cacgtcagtt tctgctttaa aacgatcagc aataatttcc
     4681 agcggatcgg tctgcccctg taaggcagtt agctcttcat gcacccgcag cataaaacgg
     4741 tgaaagtgca ggcgctgttt ccgctctccc ggcaggcttt gatagaaaag gtccatcagc
     4801 caggttttcc cgcgtcctac accgccccac atatataagc cacgcactgg cgtatgcttt
     4861 gtgtcttccc gtttacccca cagcttaccg acccgcgcca ttagcccact cgtcctgggg
     4921 gctggtggcg tgctattgat gagttcctga taaataattt ccaagcggct gacggcctct
     4981 ttttgaacgt cgtcgggttg atggctgcct tcattgagcg ccttcaggta ttgcgatgtt
     5041 ggagtaacgc tttgcatgat cttattgtta ttccttgaat aatcggtgcg ccgttgttca
     5101 cggttgacga aaaaaaggcc gttctacact acgcgatatg cagtcgggat tccacttctg
     5161 tggaattaac ggttatagtg gcataatcag ccgcaggcat ggagcctgaa gccaacaccc
     5221 tacggaaaca aaagacaacg ggagatgttc atgacctggg aatatgcgct aattgggtta
     5281 gtcgtcggca tcattattgg tgctgtggcc atgcgttttg gtaatcgtaa actacgccag
     5341 caacaggcgt tgcagtacga actggaaaag aataaagctg aactggacga gtatcgcgaa
     5401 gagctggtta gccactttgc ccgcagcgcg gaattactgg ataccatggc gcacgactat
     5461 cgccagctgt atcagcacat ggcgaaaagc tccagcagcc tgctgccgga actgtctgct
     5521 gaagcaaacc cgttccgtaa tcgtctggca gagtctgaag ccagcaacga tcaggcaccg
     5581 gtgcagatgc ctcgcgacta ttctgaaggc gcatccggcc tgctgcgtac tggcgcgaag
     5641 cgcgactaat ttatttttcg ggcgcagcca ttgcgccctc ctcttctctc cctccccgac
     5701 tatcatttaa tctggtgtct cattgttagc cgtctgaaaa ttcaataaca tcaaactgtt
     5761 ttgaatctct tttcttatca ttcaggtacg agagcaggaa taatgaaaaa acaaacccag
     5821 ctgttgagtg cattagcgtt aagtgtcggg ttaactctct cggcgtcatt tcaggccgtt
     5881 gcgtcgatcc caggccaggt tgccgatcag gcccccctcc ccagtctggc tccaatgctg
     5941 gaaaaagtgc ttccggcagt ggtgagcgta cgggtggaag gaacggccag tcagggacag
     6001 aaaatcccgg aagaattcaa aaagtttttt ggtgatgatt taccggatca acctgcacaa
     6061 cccttcgaag gtttaggctc cggtgtcatc atcaacgcca gtaaaggcta tgtgctgacc
     6121 aacaaccatg tgattaatca ggcacagaaa atcagtattc agctcaatga tgggcgcgag
     6181 tttgatgcaa aactgattgg tagcgatgac cagagcgata tcgccctgtt gcaaattcaa
     6241 aacccgagca aattaacgca aatcgctatt gccgactccg ataaattgcg cgtcggtgat
     6301 tttgccgtgg cggtcggtaa cccatttggc cttgggcaaa ccgccacctc tggcattgtt
     6361 tccgcattag gacgcagcgg gttgaatctt gaaggtctgg aaaactttat ccagacagat
     6421 gcttccatta accgcggtaa ctccggcggt gcactgttaa accttaacgg tgagttaatt
     6481 ggcatcaaca ctgcaatcct tgcgcctggc ggcgggagcg tcggaattgg atttgccatc
     6541 cccagtaata tggcgcgaac actggcgcag cagcttatcg actttggtga aatcaaacgc
     6601 ggtttgttag gcatcaaagg caccgagatg agtgccgata tcgccaaagc cttcaacctt
     6661 gacgtgcagc gtggcgcgtt tgtcagcgaa gtgttgccag gttctggctc ggcgaaagcg
     6721 ggcgtcaaaa cgggcgatat tattaccagc ctcaacggca aaccgctgaa tagctttgct
     6781 gagttgcgct ctcgtatcgc gaccaccgag ccgggcacga aagtgaagct tggcctgctg
     6841 cgtaacggca aaccactgga agtagaagtg acgctcgata ccagcacctc ttcgtcggcc
     6901 agcgctgaaa tgatcacgcc agcgctggaa ggtgcaacgt tgagcgatgg tcagctaaaa
     6961 gatggcggca aaggtattaa aatcgatgaa gttgtcaaag gaagcccagc tgctcaggct
     7021 ggcttgcaaa aagacgatgt gatcattggc gtcaaccgcg atcgggtgaa ctcgattgct
     7081 gaaatgcgta aagtgctggc ggcaaaaccg gccatcatcg ccctgcaaat tgtacgcggc
     7141 aatgaaagca tctatctgct gatgcgttaa tgtcgtaaac cgggcatcag gctgacgtgt
     7201 gatgtccggt taactcgtgg tatgctgctg ccgttccctt ttttaatgac gcctccatca
     7261 tgtttgtgaa gctcttacgt tccgttgcga ttggattaat tgtcggcgct attctgctgg
     7321 ttgccatgcc ttcgctgcgc agccttaacc cgctttccac tccgcaattt gacagtaccg
     7381 atgagacgcc tgccagttat aatctggcgg ttcgccgcgc cgcgccagcg gtggttaacg
     7441 tttacaaccg tggtttgaac accaactctc acaaccagct tgagatccgc accctgggat
     7501 ccggtgtaat catggatcaa cgcggttata tcatcaccaa taaacacgtc atcaacgacg
     7561 ccgatcagat catcgtcgcc ttacaggatg gacgtgtatt tgaagcattg ctggtgggat
     7621 ctgactctct aaccgatctg gcggtactta aaattaatgc cactggcggt ttacctacca
     7681 ttccaattaa tgcacgtcgc gtaccgcaca ttggcgacgt ggtactggcg atcggtaacc
     7741 cgtacaacct cgggcagacc attacccagg ggattattag tgctacgggt cgaatcggtc
     7801 tgaacccgac cgggcggcaa aacttcctac aaaccgatgc ttccattaac cacggtaact
     7861 ctggcggcgc gctggtgaac tcgctgggcg aactgatggg cattaacacg ctgtcgtttg
     7921 ataagagtaa cgatggcgaa acgccggaag gtatcggctt tgcgattcct ttccagttag
     7981 caaccaaaat tatggataag ctgatccgcg atggtcgcgt gatccgcggc tacattggta
     8041 tcggcggacg cgagatcgca ccactgcacg cgcagggcgg tggtatagat caactgcaag
     8101 ggatcgtggt taatgaagtg tcacctgacg gcccggcggc gaatgcgggt attcaggtca
     8161 acgatctgat tatttcggtg gataacaaac cggccatctc tgctctggag acgatggatc
     8221 aggtggcaga aattcgccct ggttcggtga tcccggttgt agtgatgcgt gatgataagc
     8281 agttaacgct gcaggtcacc attcaggaat atccggcaac caattaaggc cagcgtgaaa
     8341 gacaaaaaaa ccggagtctg tgctccggtt ttttattatc cgctaatcaa ttacttatta
     8401 acgaactctt cgcccagggc gatatctttc ttgagcgtat ccagcatacc ttccagcgcg
     8461 ttctgttcaa atgcgctcag ggtaccgata gatttacgct cttccacgcc gtttttaccc
     8521 agcagaagcg gctgagagaa gaaacgggcg tactgaccgt cgccttcaac gtaggcacat
     8581 tcgacaacgc cttgttcgcc ctgcagtgcg cgaaccagag acaggccaaa acgtgcagca
     8641 gcctggccca tagacagggt tgccgacccg ccaccggctt tcgcttcaac cacctcagta
     8701 cccgcgttct ggatacgttt ggtcagatca gccacttcct gctcggtaaa actaacgcca
     8761 ggaacctgtg acaacagcgg cagaatggta acaccagagt gaccgccaat aaccggcact
     8821 tcaacttcgc ctggctgttt gcctttcagt tccgcaacaa aggtgttgga acgaatgata
     8881 tccagcgtgg taacgccgaa cagtttgttt ttgtcataaa caccggcttt tttcagcact
     8941 tcagcagcaa tcgcaactgt ggtgttaacc gggttagtga taataccaat gcacgctttc
     9001 gggcaggttt tcgcaacttg ctgtaccagg tttttcacga tgccggcgtt aacattaaac
     9061 aggtcggaac gatccatacc cggtttacgc gctacacctg cagagataag aacgacatct
     9121 gcgccttcca gcgccggagt cgcatcttca ccagaaaaac ctttgatttt cacagcagta
     9181 gggatatggc tcagatcgac ggccacaccg ggagtcactg gagcgatatc atacagagag
     9241 agttctgaac ctgaaggcag ttgggttttt aacagtagtg caagcgcctg gccaataccg
     9301 ccagcagcgc cgaggactgc gactttcatc ctaaactcct tattatattg ataaactaag
     9361 atatgttgct ccgctgccgc gaccttaatc cacaaaattg ccgtttacaa tgaccacatc
     9421 tcaagaatgt gtagtcacgc aagtttagcg tttatgcatt taattgccgt aatcaggaac
     9481 ctaagttacg taattaacaa tcgctt
//
LOCUS       DBFIFM010000172         9427 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.172, whole genome shotgun sequence.
ACCESSION   DBFIFM010000172 DBFIFM010000000
VERSION     DBFIFM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.172"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            254..1669
                     /gene="tnaA"
                     /locus_tag="N7C74_003701"
     CDS             254..1669
                     /gene="tnaA"
                     /locus_tag="N7C74_003701"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HGU2433494.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENASNIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1760..3007
                     /gene="tnaB"
                     /locus_tag="N7C74_003702"
     CDS             1760..3007
                     /gene="tnaB"
                     /locus_tag="N7C74_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HGU2433495.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFLVDYSILRDATSSTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3139..4314
                     /gene="mdtL"
                     /locus_tag="N7C74_003703"
     CDS             3139..4314
                     /gene="mdtL"
                     /locus_tag="N7C74_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HGU2433496.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIITSVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAIMGIAVLMLSLFILKETRPAAPAASDKSRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLTVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGISAWNMLIGILIACSIVSLLLIMFVAPGRPVTAHEEIHHHA"
     gene            4289..5248
                     /gene="yidZ"
                     /locus_tag="N7C74_003704"
     CDS             4289..5248
                     /gene="yidZ"
                     /locus_tag="N7C74_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HGU2433497.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKQIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEKWDLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRDTIKNLYASMA"
     gene            5405..6154
                     /locus_tag="N7C74_003705"
     CDS             5405..6154
                     /locus_tag="N7C74_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HGU2433498.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWVSKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTMKAFSPN"
     gene            6176..6742
                     /gene="yieF"
                     /locus_tag="N7C74_003706"
                     /gene_synonym="chrR"
     CDS             6176..6742
                     /gene="yieF"
                     /locus_tag="N7C74_003706"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HGU2433499.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6796..8133)
                     /gene="adeP"
                     /locus_tag="N7C74_003707"
     CDS             complement(6796..8133)
                     /gene="adeP"
                     /locus_tag="N7C74_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HGU2433500.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRIRDLSPCVIIVALLFILKIVFIDAH"
     gene            8299..8964
                     /gene="yieH"
                     /locus_tag="N7C74_003708"
     CDS             8299..8964
                     /gene="yieH"
                     /locus_tag="N7C74_003708"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HGU2433501.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLKEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELSQLPALWKARGWN
                     ITR"
     gene            complement(9101..>9427)
                     /locus_tag="N7C74_003709"
     CDS             complement(9101..>9427)
                     /locus_tag="N7C74_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HGU2433502.1"
                     /translation="RASIKDYDDLLREIKELIISRNKSLEDKRTLFDNKRDNWNSIEV
                     QDEVNALNAKIVDCDDKIRSKLTIVRNNRLENQYKDDKNISDAMRNILDWFERYPDIV
                     QNITQA"
BASE COUNT         2321 a         2349 c         2322 g         2435 t
ORIGIN      
        1 attgacaaca agattgtcga tcaccgccct tgatttgccc ttctgtagcc atcaccagag
       61 ccaaaccgat tagattcaat gtgatctatt tgtttgctat atcttaaatt tgccttttgc
      121 aaaggccatc tctcgtttat ttacttgtgt tagtaaatga tggtgcttgc atatatacat
      181 ggcgaattaa tggatattgc agatgcaata ttcacaggga tcactgtaat taaaataaat
      241 gaaggattat gtaatggaaa actttaaaca tctccctgaa ccgttccgca ttcgtgttat
      301 tgagccagta aaacgtacca ctcgcgctta tcgtgaagag gcaattatta aatccggtat
      361 gaacccgttc ctgctggata gcgaagatgt gtttatcgat ttactgaccg acagcggcac
      421 cggggcggtg acgcagagta tgcaggccgc gatgatgcgc ggcgacgaag cttacagcgg
      481 cagccgcagc tactatgcgt tagccgagtc agtgaaaaat atctttggtt atcaatacac
      541 cattccgact caccagggcc gtggtgcaga gcaaatctat attccagtac tgattaaaaa
      601 gcgcgagcag gaaaaaggcc tggatcgcag caaaatggtg gcgttctcta actatttctt
      661 tgataccacg cagggccata gccagattaa cggctgtacc gtgcgtaacg tctatatcaa
      721 agaagccttc gatacgggcg tgcgttacga ctttaaaggc aactttgacc ttgagggatt
      781 agaacgcggt attgaagaag ttggtccgaa taacgtgccg tatatcgttg caaccatcac
      841 cagtaactcc gcaggcggtc agccggtttc actggcaaac ttaaaagcga tgtacagcat
      901 cgcgaagaaa tatgatattc cggtggtaat ggactccgcg cgctttgctg aaaacgccta
      961 tttcatcaag cagcgtgaag cagaatacaa agactggacc atcgagcaga tcacccgcga
     1021 aacctacaaa tatgccgata tgctggcgat gtccgccaag aaagatgcga tggtgccgat
     1081 gggcggcttg ctgtgcatga aagacgacag tttctttgat gtgtacaccg agtgcagaac
     1141 cctttgcgta gttcaggaag gcttcccgac gtatggcggc ctggaaggcg gcgcgatgga
     1201 gcgtctggcc gtaggtctgt atgacggcat gaatctggac tggctggctt atcgtattgc
     1261 gcaggtgcag tatctggtcg atggtctgga agagattggc gttgtctgcc agcaggcggg
     1321 cggtcacgcg gcattcgttg atgccggtaa actgcttccg catatcccgg cagatcagtt
     1381 cccggcacag gcgctggcct gcgagctgta taaagtcgcc ggtatccgcg cggtagaaat
     1441 tggctctttc ctgttaggcc gcgatccgaa aaccggtaaa caactgccat gcccggctga
     1501 actgctgcgt ttaaccattc cgcgcgcaac atatactcaa acacatatgg acttcattat
     1561 tgaagccttc aaacatgtga aagagaacgc gtcgaatatt aaagggttaa cctttaccta
     1621 cgaaccaaaa gtattgcgtc acttcaccgc aaaactgaaa gaagtttaat taatactact
     1681 gagtggctat aaggatgtta gccactctct taccctacat cctcaataac aaaaatagcc
     1741 ttcctctaaa ggtggcatca tgactgatca agctgaaaaa aagcactctg cattttgggg
     1801 tgttatggtt atagcaggta cagtaatcgg tggaggtatg tttgctttac ctgtggatct
     1861 tgccggtgcc tggtttttct ggggtgcctt tatccttatc attgcctggt tttcaatgct
     1921 tcattccggg ttattgttat tagaagcaaa tttaaattat cccgtcggct ccagttttaa
     1981 caccatcacc aaagatttaa tcggtaacac ctggaacatt atcagcggta ttaccgttgc
     2041 cttcgttctc tatatcctca cttatgccta tatctctgct aatggtgcga tcattagtga
     2101 aacgatatca atgaatttgg gttatcacgc taatccacgt attgtcggga tctgcacagc
     2161 cattttcgtt gccagcgtat tgtggataag ctcgttagcc gccagtcgta ttacctcatt
     2221 gttcctcggg ctgaagatta tctcctttgt gatcgtgttt ggttctttct tcttcctggt
     2281 cgattactcc attctgcgcg atgccaccag ctccactgcg ggaacgtctt acttcccgta
     2341 tatctttatg gctttgccgg tgtgtctggc gtcatttggt ttccacggca atattcccag
     2401 cctgattatt tgctatggca aacgcaaaga taagctaatc aaaagcgtgg tcttcggttc
     2461 gctgctggcg ctggtgattt atctcttctg gctctattgc accatgggga atattccgcg
     2521 agaaagcttt aaggcgatta tttcctcagg cggcaacgtt gattcactgg tgaaatcgtt
     2581 cctcggcacc aaacagcacg gcattatcga gttttgcctg ctggtgttct ccaacttagc
     2641 tgtcgccagc tcgttttttg gtgtcacgct gggattgttc gattatctgg cggacctgtt
     2701 taagattgat aactcccacg gcgggcgttt caaaaccgtg ctgttaacct tcctgccacc
     2761 cgcgttgttg tatctgatct tcccgaacgg ctttatttac gggatcggcg gtgccggact
     2821 gtgcgccact atttgggcgg tcattattcc cgcagtgctg gcaatcaaag ctcgcaaaaa
     2881 gtttcccaat cagatgttca cggtctgggg cggcaatctt attccggcga ttgtcattct
     2941 ctttggtata accgtaattt tgtgctggtt cggcaacgtc tttaacgtgt tacctaaatt
     3001 tggctaaatc cttcaagaag ccagtcattc gctggcttct tgcctctcag gaaatcactt
     3061 atgtccaaat ggcaactcgc ctgatcctcc ttcaccacgt atgctttgcg tcaccttact
     3121 atcaggacgc tttagcccat gtcccgcttt ttgatttgta gttttgccct ggttttactt
     3181 tatcccgctg ggattgatat gtacctcgtt ggtttaccgc gcatcgccgc cgatctcaat
     3241 gccagcgaag cgcagttaca tattgcgttc tccgtttatc tggcaggtat ggcagctgcg
     3301 atgttatttg ccggtaaagt ggccgatcgt tcagggagaa aaccggtcgc catccccggc
     3361 gcggcgctat ttattatcac ttcggtgttc tgttcactgg ctgaaaccag cacgttattt
     3421 cttgcagggc gctttctaca ggggttgggc gcgggctgtt gttacgtggt ggcattcgcc
     3481 attttgcgcg acacgctgga tgatcgacgt cgggcaaaag tgctgtcgtt actcaacggc
     3541 attacctgca tcattccggt gttagcgccg gtgctcggtc atctgattat gcttaaattc
     3601 ccgtggcaga gtctgttctg gacgatggca ataatgggca tcgcggtact gatgttgtct
     3661 ttgtttattt taaaagaaac gcgccctgcc gcccccgcag cttcggacaa atcacgagaa
     3721 aatagcgagt cgctgcttaa tcggtttttc ctcagccgtg ttgttatcac caccctcagc
     3781 gtttcggtga tcctcacctt cgtcaataca tcgccggtat tgctgatgga aatcatgggt
     3841 tttgagcgcg gagaatacgc caccattatg gcgttgaccg ctggcgtcag catgaccgtt
     3901 tcattttcca cgccatttgc gctgggaatt tttaagccac gtacgttgat gatcacctca
     3961 caggtgttat tcctggcggc gggcatcact cttaccgttt caccttccca tgcggtttct
     4021 ctgtttggta tcacgctgat ttgcgccggt ttctcggtag gttttggtgt ggcgatgagt
     4081 caggcgttag ggccgttttc attacgcgcg ggcgtagcca gctcgacctt aggtattgcg
     4141 caggtttgcg gttcgtcact gtggatttgg ctggcagcgg tggttggtat cagcgcatgg
     4201 aatatgctga tcgggattct gattgcctgt agcatagtga gcctgttgct gattatgttc
     4261 gtcgcgcctg gacgcccggt taccgctcat gaagaaatcc atcaccacgc ttgatctcaa
     4321 tctgctgctc tgtctgcagc tgctgatgca ggagcgcagc gtaaccaaag cggcgaagcg
     4381 gatgaacgtg acaccttcgg cggtgagtaa gtcgctggca aagttaagag cgtggtttga
     4441 cgacccgctc tttgtgaact caccgttggg gctgtcgccc acaccgctaa tggtcagcat
     4501 ggagcaaaat ctggcggagt ggatgcaaat gagcaacctg ctgctggata aaccgcacca
     4561 ccagacaccg cgcggcctga agtttgagct ggcggcagaa tcaccgctaa tgatgatcat
     4621 gcttaatgcg ctgtcgaaac agatctacca acgttacccg caggcgacca tcaaattacg
     4681 taactgggat tacgattcct tagatgccat tactcgtggt gaagtggata tcggtttttc
     4741 cggtcgcgaa agtcatcccc gttcgcggga gctgttaagc tcgctaccgt tagccattga
     4801 ttatgaagtg ttgtttagtg atgtgccctg cgtctggtta cgcaaagatc atccggcact
     4861 gcatgaaaag tgggatctcg ataccttttt acgttatccg catatcagca tttgctggga
     4921 acagagcgat acctgggcgc tggacaatgt gttacaggag ctggggcgcg aacgtacgat
     4981 tgcaatgagc ctgccggaat tcgagcagtc actgtttatg gcagcgcagc ccgacaatct
     5041 gctcctggcg accgcgccgc gctactgtca gtactacaat caactccacc aactgccgtt
     5101 ggttgctctt cctctcccgt ttgacgaaag ccagcaaaaa aagctggaag tcccttttac
     5161 cctgctgtgg cataaacgga acagccataa tccgaagatc gtctggttac gggataccat
     5221 taaaaatctt tacgcgtcga tggcataacc gaatcgtatg aaacgcgacc cgatttcaca
     5281 ataaaatgta aaaaagttgt aataagcttg tctgaatcga acttttagcc gctttagtct
     5341 gtccatcatt ccagtaaatg attactcttg tattcataat ggaccattaa gcatggagcg
     5401 aaaaatggcg actcactttg cccgagggat tttaacggaa ggacatctga tttctgttcg
     5461 tctcccctcc cagtgtcatc aagaagcccg aaacattccc cctcatcgtc aaagccgttt
     5521 tctggcgtcc agaggtttac tcgcagaact gatgttcatg ctgtatggca ttggcgaatt
     5581 gccggaaatc gtcaccctgc cgaaaggtaa accggttttc agtgataaaa atttgccttc
     5641 gttttctatt tcctatgccg ggaatatggt tggcgtggcg ttaacaaccg aaggtgaatg
     5701 tggcctcgat atggaactgc agcgtgcgac gcgcgggttt catagcccac acgcgcccga
     5761 taaccacaca ttttccagca atgaatcgct atgggtcagt aaacaaaatg atccaaacga
     5821 agcgcgggcg cagctcatca cgctgcgccg aagcgtgcta aaactaaccg gtgatgtttt
     5881 gaatgacgat ccgcgcgatc tgcaactgct gcccattgcc ggacgcctga aatgtgctca
     5941 tgtaaatcat gtagaagcgt tatgcgacgc ggaggacgtg ctggtgtggt ccgtggcggt
     6001 cacgcccacg attgaaaagc tcagtgtctg ggagttagat ggcaaacacg gctggaaaag
     6061 cctgccggat attcacagcc gcgccaacaa tcctaccagc cggatgatgc gttttgccca
     6121 gctctctacc atgaaggctt tttcgccaaa ttgatagaca accacaggag tcattatgtc
     6181 tgaaaaattg caggtggtta cgttactggg gagcctgcgc aaaggctcat ttaatggcat
     6241 ggttgcacgt accctaccga aaattgctcc ggcgagcatg gaagtcaatg cgttaccttc
     6301 cattgccgac attcccctgt atgacgctga cgtacagcag gaagaaggtt ttccagcaac
     6361 ggttgaagct ctggcggaac agatccgtca ggctgacggt gtggtgatcg tcacgccgga
     6421 atataactat tcggtaccgg gtgggctgaa aaatgccatc gactggcttt cccgcctgcc
     6481 ggatcaaccg ctggccggta aaccggtgtt gattcagacc agctcaatgg gcgtgattgg
     6541 cggcgcgcgc tgtcagtatc acctgcgcca gattctggtt ttcctcgatg caatggtgat
     6601 gaacaagccg gaatttatgg gcggcgtgat tcagaacaaa gttgatccgc aaaccggaga
     6661 agtgattgat cagggtacgc tggaccacct gaccgggcaa ttgaccgcat ttggcgagtt
     6721 tattcagcga gttaagatct aaataaaaaa cccgccagca atcatgcatg gcgggttttt
     6781 aacgcgctat cgattttagt gagcgtcgat aaatacaatc ttcaggataa acagcagcgc
     6841 aacgatgatt acgcacgggc taaggtcacg aatacgcccg gtaccaatct tcatcacgca
     6901 gtaggagata aagcccagcg caataccttc ggtaatcgag aagctgaacg gcatcatcac
     6961 agcggtaata aacgccggaa cagattcagt aagatcctgc cagttcacgc gagcaagact
     7021 tgaggtcatc aacacgccaa cgtaaatcag cgcgccagct gcagcgtagc ctggcaccat
     7081 tcccgccagc ggcgacagaa agataaccag caggaacagc agaccaacaa ccactgccgt
     7141 cagaccggta cgaccgccaa ccgatacgcc ggaagaggac tcaatataag ccgtaacgga
     7201 agaagtaccg ataaacgaac cggtcacgga agagatactg tcgacataca gcgcctgctt
     7261 catgcgcggg aatttcccct tctcatctgc cagaccggct ttatcggtca cgccaatcaa
     7321 cgtgccggag gagtcaaaca ggttgaccag catgaaagag aaaatcaccc ctgccagccc
     7381 gaggttaaac gacccggcta aatctacatg acccacgaca gtcattacgc tcggcggcgc
     7441 agaaacgatg ccattgtagt ggacatcacc cagcatccag cccagcagcg ttgtcaccac
     7501 gatagaaacc agcaccgctg cgtgaatgtt gcgtgaggcc agaatagcaa tgatgaagaa
     7561 gccgaggatc cccagcagca cgctgtgaga agtcagatta ccgatgctga ccagcgtttc
     7621 cgggttagcg acaatcacac ctgcgttttt cagccccatc atgccaatga acagaccgat
     7681 accgctggta atgcccacac gcagactcac cggaatgttg gcaatcatcc agtagcgaac
     7741 gcggaaaatc gtcagtaaca gcagaccaat cgcgccccag aagattgcgc ccatcccgac
     7801 ttgccacggc aagcccatcg cctgcacaac gacaaaagcg aagaacgcat tcaggcccat
     7861 agcgggtgcc agtgcaactg gcaggttagc aaacagcccc atcataatac tgccgaatgc
     7921 agcaatcaga caggtagtga cgaagacggc gctggtatcc atgccagcaa cgccaagaat
     7981 ttgcgggtta acaaaaacga tgtaaaccat cgtcaggaag gtggtaaaac cggcgatcac
     8041 ttcggtccgt gccgtcgtgc catgttcgcg cagtttaaac acgcgttcca gcatcccctg
     8101 gccagaagcc tgggttgtgt gttgttgact cattatctat ttccgaacaa ggagggaaaa
     8161 tccgtcgcta tcgtatacca aaatgagaca atatggacgg ctgaagataa cttttttcat
     8221 tgtatttgct tatacggcaa cgattgcgtc atgcgaattg gcatgacgaa aacgttaaac
     8281 taacgataaa ggaaagacat gtcccagata gaagcggtat ttttcgactg cgacggtacg
     8341 ctggtcgata gcgaggtcat ttgctctcgc gcctatgtca cgatgttcag ggaatttggc
     8401 attcacgttg acctgaaaga gatttttacc cgttttaaag gcgtaaaact ctacgaaatc
     8461 atcgatatca tcagtaaaga acagggcgtg acgatggtcc aggccgatgc cgagcacgtt
     8521 taccgcgcag aagtagcacg tctgtttgat accgaacttg aggacatcgc aggagcgaac
     8581 gaattgctgg cgagcattaa cgtgccgatg tgcgtcgtct ctaacggtcc ggtcagcaaa
     8641 atgcagcatt cgcttggtaa gctcaacatg ctgcactact tcccggaaaa actgttcagc
     8701 ggatatgaca ttcagcgttg gaagcccgat ccggcattaa tgttccatgc ggcaaaagcg
     8761 atgaatgtga acgttgaaaa ctgcattctg gtcgatgact ccagcgctgg cgctcaatca
     8821 gggatagatg ccggaatgga agtgttttac ttctgcgctg acccgcacaa taagccgatc
     8881 gttcacccga aagtcaccac ctttaccgag ctgtcgcagc tgcctgcgct ctggaaggca
     8941 cgcggctgga atataacgcg ataatcgcct ccttacttca acacttacac atcagtcacg
     9001 ctggtttgag atacagaaca ccaggtgtct gatgagtaag cgtctattcc ctatgaataa
     9061 tttatgtcgt tattcgtcct gtcatccacc ctctgtatgg ttaagcctgg gttatatttt
     9121 gtacaatatc gggataccgt tcgaaccaat ccagaatatt acgcatcgcg tcactgatgt
     9181 ttttatcgtc tttatattga ttctcaagtc tattatttcg aacaatagta agcttagaac
     9241 gtattttatc atcacaatcg acaattttcg cattcaatgc atttacttca tcctgcactt
     9301 ctatactatt ccaattgtcc cttttgttat caaataaagt ccttttatcc tctaatgatt
     9361 tattacgact tatgattaac tctttaattt ctcgcaataa gtcatcgtaa tctttaatgg
     9421 aagctct
//
LOCUS       DBFIFM010000173         9275 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.173, whole genome shotgun sequence.
ACCESSION   DBFIFM010000173 DBFIFM010000000
VERSION     DBFIFM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.173"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..157
                     /gene="ghoS"
                     /locus_tag="N7C74_003710"
     CDS             <1..157
                     /gene="ghoS"
                     /locus_tag="N7C74_003710"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HGU2433503.1"
                     /translation="RQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDD
                     YFRQFYK"
     gene            185..358
                     /gene="ghoT"
                     /locus_tag="N7C74_003711"
     CDS             185..358
                     /gene="ghoT"
                     /locus_tag="N7C74_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HGU2433504.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(477..1994)
                     /gene="lysS"
                     /locus_tag="N7C74_003712"
     CDS             complement(477..1994)
                     /gene="lysS"
                     /locus_tag="N7C74_003712"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGU2433505.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(2231..3688)
                     /gene="dtpC"
                     /locus_tag="N7C74_003713"
     CDS             complement(2231..3688)
                     /gene="dtpC"
                     /locus_tag="N7C74_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HGU2433506.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESH
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMVSGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMVQESND"
     gene            complement(3747..5894)
                     /gene="cadA"
                     /locus_tag="N7C74_003714"
     CDS             complement(3747..5894)
                     /gene="cadA"
                     /locus_tag="N7C74_003714"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HGU2433507.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(5974..7308)
                     /gene="cadB"
                     /locus_tag="N7C74_003715"
     CDS             complement(5974..7308)
                     /gene="cadB"
                     /locus_tag="N7C74_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HGU2433508.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(7674..9212)
                     /gene="cadC"
                     /locus_tag="N7C74_003716"
     CDS             complement(7674..9212)
                     /gene="cadC"
                     /locus_tag="N7C74_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HGU2433509.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
BASE COUNT         2617 a         2173 c         2107 g         2378 t
ORIGIN      
        1 acgacaattg aatgaaaatt ccttcgcaat aacgacatcg ttagccgcaa gtgaaatcga
       61 agatttaatc aggcttaaat gcttagactt accggatatt gactttgacc tcaacattat
      121 gacagttgat gactatttcc gtcagtttta caagtagcca gtaaggataa gaagaacata
      181 aaaaatggca ctattctcta aaatattaat tttttatgtg attggtgtga acatatcctt
      241 tgtcattatc tggtttatct cacatgagaa aacacatatt cgtttactta gtgcattcct
      301 ggtcggaata acctggccaa tgagtctgcc tgtggcatta cttttttctc tcttttagga
      361 gctgtagttg agtggacccg ctggttatcg tgaaacgaag cgtctctcct caaaactgga
      421 tttgcggaac gatgaacgaa gcagtcgcct gactgcttcg ttcattaaag tgaaatttat
      481 ttctgtgggc gcatcgccgg gaagagaata acgtcgcgaa tagtatggct gttagtaaac
      541 agcataatca ttcggtcgat accaataccc agaccagcgg ttggcggcag accatattcc
      601 agcgcagtca cgtaatcttc gtcatagaac atggcttcgt cgtcacctgc ggctttagcg
      661 ttaacctgtt cctggaaacg ttcagcctga tcttctgcgt cgtttaattc gctaaaacca
      721 ttaccgattt cacgaccacc gatgaagaat tcaaaacggt cggtgatttc cgggttaaca
      781 tcattacggc gtgccagcgg tgacacttct gccggatatt ccgtaataaa ggttggctga
      841 atcagatgtg cttctgccac ttcatcaaag atctccgtga caatacgtcc cagcccccag
      901 cttttctcta ccgtaatacc gatagattca gctaatgctt tagcagcatc aaaattatcc
      961 aggtcggcca tatcggtttc tggacgatat tttttgattg cttcgcgcat ggtgagtttt
     1021 tcaaacggtt tgccgaaatc aaacacatgc tcgccataag tgactttagt ggtacccaga
     1081 acctcttgtg ccagggtgcg gaacagggac tctgtcagtt caatcaaatc gtggtaatcc
     1141 gcatacgcca tgtagagttc catcattgtg aactcaggat tatggcgaac agaaatacct
     1201 tcattacgga agttacggtt gatttcgaat acccgttcaa aaccgcctac aaccagacgt
     1261 ttcagataca gctccggcgc gatacgcaga tacatatcta aatccagagc attatgatgg
     1321 gtaataaacg ggcgagcaga tgccccacct ggaattacct gcatcatcgg ggtttctact
     1381 tccataaagc cgcgcgcgac catgaactgg cggatagcgg ccagaatttt tgaacggaca
     1441 acaaacgttt gacgggattt atcgttagcg atgaggtcca gataacgttg acgataacgg
     1501 acttcctgat cctgcagacc atggaattta tctggtaaag gacgtagtgc tttagtcagc
     1561 aggcgcaact cagtacagtg aatggaaagc tcacccgttt gcgtcttaaa cagcgtaccg
     1621 cgggcaccga taatgtcacc cagatcccat tttttaaact gatcgttata aacaccttct
     1681 ggcaggctat ctcgtgcgac gtacagttga atacggccac cgacatcctg caacgttaca
     1741 aaggaggctt tccccatgat acgacgggtc atcattcggc cagcaaccga gacttcaatg
     1801 tttaaggatt ccagttcctg gttatccttc gcatcaaact cttcgtgcaa ctggtcagag
     1861 gtatggtcgc ggcgaaaatc attgggaaac gccacacctt gctgacgtag tgccgccagt
     1921 ttttcgcggc gatttctcag ttcatcgtta aaatcaatag cctcattggc tccccgtgtt
     1981 tcttgttcag acattttggt tcctctaaat ccagctttca atttagcgta gataaagaga
     2041 cagatcggta cactgtatta tctgcctcga ctattaataa ctcagtttat cttatcgaaa
     2101 ctaacgctta cctcaagttg atatcactaa taaataacca tcaaaatggt ttaaatgact
     2161 tattaatctc gatttactag tgtgataaac tgaaagttaa tttattcata accaattgtt
     2221 tttacgacag ttaatcgttg ctctcctgta ccatattcgt cggcgtgcta aacaggaaac
     2281 gggtagcaaa ggccaatacc acgatgatcg cgacacaagc caacgtccac tctcccatct
     2341 gagaaaagaa tcgctggtaa gctgcaattg ccataccgct aatttgcgac tctgtcgtct
     2401 gctgtgccac tacgcctgcc agccagttgg cgaccgcgcc tgttgccagc atataaatcc
     2461 cggttaatac gccagacatt ttcagacgcg tgatttgcgc aatcgccacc gggtcaataa
     2521 agagttcggc aaagcccatt agcgccagcc cggataccat cacgcccatt gacgcttgac
     2581 cgtcagctgc tgcatgtcgg gcatcaaatg ccagcaacat aaagccacaa gccatcagca
     2641 gtaagccaaa ggcaaacttc agccagacgc gcaatgttga gttgccgtgg ctttctggcg
     2701 acgccagcca ggccagaaca accccagcga gcatcaccgc aattgcattc accgactgga
     2761 atagtgcagt aggtacttca atattgaatg cctgacgatt cacaaagcga tcgataaaca
     2821 agctgatggt actgccgccc tgttgtgcca gtacccagaa caatgtcccg acaaacatca
     2881 acaatacaat ttgccaaaga gcacggcggt gttcggggaa tttgatcatc atgcgggcaa
     2941 tgatttgtgc ggcaatgagg caaacgatcg ccagcaaata tccggaccag tcgttctcca
     3001 gcagcagggt aaaaaatact ggggctaaac agagcatcac cactaaccag ctccataccg
     3061 gtaaggcaaa tttgacgcta gtgagcgctt ttttatccat actacgcgtg gactggaaat
     3121 gacgatgccc gcttaagaaa atcaacaaac cgataaacat gccgccaccc gcaagggcaa
     3181 agccaacatg ccatccatac cactgagcag ccaggccgca ggcgattggg gcggcgatag
     3241 aaccgatatt gcccgcagca tacagcagcg aaaaaccgcc atcacgtcga tgatcgttct
     3301 cgtcgtagag ctcgccaagc aaacagctga tgtttgattt gaataaaccg tagccacaaa
     3361 taatgattgc cagcgccaga tacaggctaa aggttgaatt tgtatcaata cccagcacca
     3421 catggccaag ggtcattaac agcgcgccgg caatcactgc agtgcggttg ccgagcaggc
     3481 ggtcggcaag ccagccgccg agaatagggg taacgtaaac cagagaagca tatgcgctga
     3541 acaggctgat ggcatggtta tcatcaaaac caagctgatg ggtgagatag agaatgagta
     3601 aggcacgcat gccgtaaaaa ctgaagtact cccagatttg gatcgccacg atatagtata
     3661 tcgcgcgcgg ctgtgagggt gttttcatgt gttctcctta tgagcaaaaa agggaagtgg
     3721 caagccactt cccttgtacg agctaattat tttttgcttt cttctttcaa taccttaacg
     3781 gtatagcggc catcagcctg acggtatgca ccgtgaatat cggtttcaaa gcccggatag
     3841 tgagcgccga tttcacacag catctgcagg aactccagaa ccggacggct ttcttcggtg
     3901 atcatttcac ccggcattac cagaggaact cccggcggat acggaaggat catattggcg
     3961 ttaatacgac cgaccatttc gtcgaggtaa acttcttcgg tcataccgtg cagctctttc
     4021 tggaatgcag catacggagt cattaccatc gtcggcagca cttcaaatgc gcgatacatc
     4081 agatccggca gattgtggtg aacaatcagt ttgtggatat tctgagccag ttcctgaata
     4141 cgcatgtttt catagaattc aggatcttca cgatacagag acggcagcat gtttttcaca
     4201 cgcaggttca ggtcgaacgc acgtttgaag tcagtcagag cacgcagcag gctcagtgct
     4261 ttggtcttat cgataccgat gctgaacagg aacagcaggt tatacggacc ggttttctca
     4321 acaacgatgc catgttcgtc gaggtatttc gccacgatgc tggccggaat accaaagtcg
     4381 ctcatggtgc cgtctttttc catccccgga gtcagcaggg tgactttgat cgggtcaaga
     4441 tacatgtgct cgttatcgat gtttttgaag ccgtgccagg tgctgtcaga acgcagcggc
     4501 cagcattcag tcgtatcgat atgatccggc tgccatacat caaagaacca gccatcagat
     4561 tccgttctca gacgtttgat ctctttacgg aatttgatcg cacgttcaat ggaaccgttg
     4621 atcagacgct tacctgcatt gcctttcatc atcgccgcag cggtttcagt ggacgccacg
     4681 ataccgtagt gcggagaagt ggtggtgtgc atcatgtagg cttcgttaaa ggtttcttcg
     4741 tttacgtcac ctttaacgtg gatcatggaa gcctgagaga acgccgccag cagtttgtga
     4801 gtggactggg tttcgtaaat cactttccct tctacacggc caccgctcat accgcattta
     4861 ccttcgtaaa tcggtgagaa gttggtgtaa ggcacccacg cggagtcaaa gtggatggat
     4921 ttcacatcca gtgttttctt gatgaagtcg gtgttgtaca gcagaccatc ataggtagag
     4981 ttggtaatta cagcatgtac cggccaggtt gcgtttggtg tttctttcac gcgcttagca
     5041 atggtagcgt gctggaattc actctgtggg ataccaccaa gaataccgta agcgttacgg
     5101 gtcgggcgga aatagattgg cgtaacatcg ctcatcatca tcaggtgggt cagcgatttg
     5161 tggcagttac ggtcaatcag aatggtgctg cctgccggag cagagtacat accaacaatt
     5221 ttgttcgcag tggaagtacc gttggtcacc atgtagctgc ggtctgcgtt aaagacgcga
     5281 gcgatatact gttctgcttc tttgtgtgga ccactgtgat ccagcagaga acccagttca
     5341 gatactgaaa tggaaatatc agatttcatg gtattcggac caaagaaatc atagaacagg
     5401 ctacctaccg ggcttttctg gaatgcagta ccacccatgt gaccaggagt acagaaagta
     5461 tatttacctt cacgaacata tttaaacagt gctttagtca gcggaggcag aatagtgttg
     5521 atatattcgt cagtggtctg cttgatcttg ttagcaatat cttcagcagc acccagcgca
     5581 tattcaaaga agctaatctg taaacgcagg tcattcaggc ttacatcgag agtggaatac
     5641 gtattagcga acgcgtacaa cggcaggttc tcgttcattt tgctaatttc ttcgcacagc
     5701 tcgagattat atttatccca gtcaaaaata acgccgcaca gacgcgcatt gttttcgatc
     5761 agttttaata agtcgtcacg gtcgttcggg taaacaatct ggaagttcag acgttcaagc
     5821 gcgcgatgaa gttcacggat gggttcttct ttaaaataaa cccccatgtg attcaatatt
     5881 gcaataacgt tcatagtcat atctccaggt aaaaaaggcc cctcccaaca catgggacaa
     5941 aatgaaagga ggagcctcgg aaaatacttt taattaatgt gcgttagacg cggtgtggtt
     6001 atccattgag tggctctggc gctcgtgcat tttgcgagcg tagaacatca ggataatcag
     6061 gctgacgatg aaggtacctg ccagctcgaa ggagcttgcg cccatcagcg cgatgaagca
     6121 gaacacgcaa cccagtacag agcagatcag gctgacaaag ttgcggatgt taacgccttc
     6181 aaaacgaatc aggtcaacgc aagagtagaa gtacggcagc atagtcagca gtactgcgat
     6241 accggtcagt tcaccgaaca ggtcagatgc tttaccaccg gcagagttca tcagagtgat
     6301 gaggatcatc agggcagtca ttttcactgc agccagcagc agaccttttt tcggaatacc
     6361 gttgctgtcg acttcaccat aaactttcgg gaagttaccg tcgttagcgg cacgtacacc
     6421 tgcctggcct accaacatca tccaggagcc cagagaagtc aggcacgcaa aggcggtgaa
     6481 tgcagaaacc agcggcgcag cccagttacc gaggatagtt gaagcactga ttgcaaacgg
     6541 agcaccggaa gccgccatta cagaagacgg atacataccg gaaagcacct gagtcgcagc
     6601 gatgtaaaca atacctgcta aaccagtacc cagcatggtt gccagcggaa cggtacgttt
     6661 cgggttttta accataccag tacttacagc tgcggattca acacccacga aggcccacag
     6721 gcagagcaga atacttttaa tgatcgcatg accatcagtg gtatccgcag tattccagtt
     6781 agctgcataa gttgccgcat caaaccaatg ccagccaaca atagcagtca tcaccacagg
     6841 aataagaacc agcaccagac caatagtggt taaacggctt acccaggtac cgccgagcat
     6901 atttacaaag gtaaataccc agacgatagc aatacaggcg atacccgccg gaacaggatc
     6961 atttaatact gggaagaagg tggaaagata agatacagcg gtaataccaa tcgccaggtt
     7021 accaatccag ttagcatggt aataaagaac acctgtctga aaaccaaatg caggagaaat
     7081 ttctccggca taagcaattg ggccaccttg ttgcgggttt tttgttgcca gtcgggcata
     7141 tacatacgcc agcgacattg caccaataat agagataatc caaccccaga tagcaatacc
     7201 accgatactt gctaggttcg caggtaataa tgcaataccg ctccccatca tattaccggc
     7261 aacaacaccg gtacaggcaa atagcccgat cttcttggca gaactcatgc tcttctccta
     7321 atttcatttt tgaatttgga gtccgggtca tgatgtataa ctatttcctg accagaccaa
     7381 actggcgcta agattactca cgaaaaaagg attaatccta aagattaggt gaaataacac
     7441 aaaagtttct gtaagtgaga acttgaggtt ttttattaac acatcaggat cgcaagttga
     7501 tatcatgaaa agataaacat tcaatgttta caatagattg cgtgacgtct tctggttaaa
     7561 tttatgtaat aaaaattatg cggcaaataa attgccgcaa catattatac caacaggaac
     7621 atacaaaaac tcaacaacaa atattcccga gcatgaatca acccgggggt tacttattct
     7681 gaagcgagaa atttgtcgag ataaggtaca acataaggaa cagaagtctg gaatatacca
     7741 ttttcaatcc agtaaagggt gtttgcccct gggcgtaaat taaaggcggt gagatatgca
     7801 tcagccgctt cccggttcat ccccttcatt tcataaacct tgccaagcaa tacataattt
     7861 agccaggaca tttcaagatc aatgccagta tttatcgcct ggtaagactc atctgtttta
     7921 ccttttacca gagcactgac cgcttttatt tgatatataa tggacaggtt attcaattcc
     7981 ggtagtgtaa caatgttatc tatttctgtg ttcagtgctg ctaattgttt ttcatctaaa
     8041 ggatgttgag aatggcgcac gatatcaact aatgcttttt ctgctctcgc gtaggtaaat
     8101 tctggggatg attgaacaat ctcacctaat aattcactgg cacggttcaa tgatttatca
     8161 tcgccatgca gtaaataatc atgtgcctga taaaaattag ttaataacgc accacgatga
     8221 ggcaaaattt gctggagcgt ctcctgcatt cgttgtggcc acggttggtt taacgctttt
     8281 gataaactct ccagtaaatc attttgaatc gccagctgat taccgttagt gatgacataa
     8341 cgtttatcca gcatggttga accatctgca ttgtctacca attttatcga cataaagcat
     8401 tgttgagcac ggtattggcg ctgattaaca aacgcaatag ataatgtttt accggaactg
     8461 ctcggttcat caatgttgta gttgattttg tcatgcacca taaaggtgga gaaggtgtta
     8521 agtgatgtcg ccaccaaatc acccacgcct atcgcgtaag agagctgata cggggaactc
     8581 cagctgttac aacttttatt taccatatta atgtcaatat cgcgtggatt gagcaaaata
     8641 cgcgatttgc tcatcggaag acgtgtatca agacttgaaa acgctaccag tgctacacag
     8701 atacctaagg acaacaggaa aaaaaaccat acccaaaagg tagtgaatcg tttgctttta
     8761 actggggatt gttcaggtgg cgttgcggtg ttttgaatgt taagactgtg ggagggagaa
     8821 tctgtggcag gaaccgcctc tggtataggg ggaggcgaag atagcattat ttcctctccc
     8881 tcttcttcgc tgtaccagat aaccggcacc attaatttat agccgcgctt tggtacagta
     8941 gcgatataga caggactatc ttcatcatta tcttttaatg acttacgtag ttctgagata
     9001 ctctgcgtca caacgtgatt ggtgacaata cttctcttcc agacattatc gataagttca
     9061 tccctgctaa gtacttcgcc actgtgttga gcaaagaaaa ccagaaggtc gattaatctc
     9121 ggctcaaggg taagttgacg cccattgcgg ctaatttggt ttatggacgg agtaacaagc
     9181 cattcgccaa cgcgaactac aggttgttgc ataatagaaa ctcattcgaa aagggaatga
     9241 tgcaatgata attgccacaa cctattttta ccatc
//
LOCUS       DBFIFM010000174         9270 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.174, whole genome shotgun sequence.
ACCESSION   DBFIFM010000174 DBFIFM010000000
VERSION     DBFIFM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.174"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            115..477
                     /locus_tag="N7C74_003717"
     CDS             115..477
                     /locus_tag="N7C74_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433510.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKSMSKGREE"
     gene            complement(656..1114)
                     /gene="ybbJ"
                     /locus_tag="N7C74_003718"
     CDS             complement(656..1114)
                     /gene="ybbJ"
                     /locus_tag="N7C74_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HGU2433511.1"
                     /translation="MMELIVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(1111..2028)
                     /gene="qmcA"
                     /locus_tag="N7C74_003719"
     CDS             complement(1111..2028)
                     /gene="qmcA"
                     /locus_tag="N7C74_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGU2433512.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            2174..2851
                     /gene="fetA"
                     /locus_tag="N7C74_003720"
     CDS             2174..2851
                     /gene="fetA"
                     /locus_tag="N7C74_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HGU2433513.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            2838..3617
                     /gene="fetB"
                     /locus_tag="N7C74_003721"
     CDS             2838..3617
                     /gene="fetB"
                     /locus_tag="N7C74_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HGU2433514.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3680..4534)
                     /gene="cnoX"
                     /locus_tag="N7C74_003722"
     CDS             complement(3680..4534)
                     /gene="cnoX"
                     /locus_tag="N7C74_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HGU2433515.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4595..5404)
                     /gene="ybbO"
                     /locus_tag="N7C74_003723"
     CDS             complement(4595..5404)
                     /gene="ybbO"
                     /locus_tag="N7C74_003723"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HGU2433516.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRRSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(5394..6017)
                     /gene="tesA"
                     /locus_tag="N7C74_003724"
     CDS             complement(5394..6017)
                     /gene="tesA"
                     /locus_tag="N7C74_003724"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HGU2433517.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFLMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            5988..6674
                     /gene="ybbA"
                     /locus_tag="N7C74_003725"
     CDS             5988..6674
                     /gene="ybbA"
                     /locus_tag="N7C74_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HGU2433518.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6671..9085
                     /gene="ybbP"
                     /locus_tag="N7C74_003726"
     CDS             6671..9085
                     /gene="ybbP"
                     /locus_tag="N7C74_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HGU2433519.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDSYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNYVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVTPLKAF
                     LAEHQIVPESFYPVVRARLTAINGKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVTLGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2121 a         2379 c         2541 g         2229 t
ORIGIN      
        1 cgaaataaat tctgtttctg gactaaacgt gaagcggttc ctgatgtgtg ctaaaatgcg
       61 tttctttgat aaatctcaat ttttaagagg tttacgccag gcaggaaaat ttaaatgaat
      121 gaatttgaga agatttttaa tgaaatgaat ctggaccggg cattgctacc gatccttttt
      181 cgttcgaacc ggtcgacagt gtggaagtat ttaagcggcg atagtacggc tcctgcctct
      241 gccatgtcgc tgattatgct gttgcagttg atacagaaac gaaaccccga tctgttagcc
      301 gaatggttaa cgctttctga tttcaccatt ccgccagaag tttacctgga ccagcctgat
      361 tactggaaag ggtgggtgta tacgcaacac aaggtcaata aaaacgtcct ggaatatcta
      421 aaaaaacact atccggatga agaccaaaaa agtatgagta aaggccggga agagtaaatt
      481 gccaatgcca gttaacattt aacatggcgg aggcggccta cggttttggc gtgatatgac
      541 gcgcccgcgt cgcatcaggc gtcggtgcac gactgccgga tgcggcgtaa acgccttatc
      601 cggcctacga ttcgggcgca gacttccggg catgataaga cgcgtcagcg tcgcatcagg
      661 cgatgaccgc acggatgatc agcgttatcc cttctatcgc aatgacttca acatgcgtac
      721 ctgcgccgag atcctcgctg gcgctgacag gccatgaact gtcaccgacg cgcatatgac
      781 cgcgcccgtt gaccagcgga gattccagca caaaacgtcg gccaatcagc tgctgcccgc
      841 gctggtttaa atgactgtcg ctgtgctttt gttcgcgcac ccgccgcgac aaccatttcc
      901 accacagcca ggcggcgagc agcgtcagga cggcaaacat caccccttgc cactcccaac
      961 ccagcggcac cagccagacc accaggccag taatcactgc tgccacgcca ctccacaaca
     1021 aataaccatt tccgcccagc atctcggctg ccagcagcaa accgccgaga ctgagccaga
     1081 aaatatgtgg atgaacgact attaactcca tcatggctga gtccgcttgt tggcgctgtc
     1141 tttcaccagc tcggcaatcc cggcaatcga ccccatcagg ctgctggcct ctaatggcat
     1201 catcactact ttgctgttac tggaggaacc gatctgctgt aacgcttcgg tgtatttctg
     1261 cgctacgaag tagttcaccg cctgaatatc accggaggcg atggcttcag acaccatttt
     1321 ggtggcgcgg gcttctgctt cagcggaacg ttcacgcgct tcagcctgta aaaacgccga
     1381 ctgacgttcg ccttccgctt tcaggatttg cgactgtttt tcaccttcgg ctttgaggat
     1441 ttccgcctga cggatccctt ccgcttcaag aatgtaagcg cgtttggtac gttccgcttt
     1501 catctgcgcg ttcattgaag agataagctc tgccggtggg cgcacgtcgc gaatttcaat
     1561 acgggtgact ttaatccccc acgggttggt ggcctcatcg acaatacgca gcaggcgtga
     1621 gttgatgctg tcgcgctgag agagcatttc gtcaagttcc attgaaccca acacggtacg
     1681 gatgttagtc atggtcaggt tgatgatcgc cagctccaga ttgctgactt cataagccgc
     1741 gcgtggcgcg tcaatcacct gaataaaaca gactgcgtcg atggtaacgt tggcgttatc
     1801 tttcgagata acttcctggg aagggatatc gagcacttgc tccatcatat tgatcttgcg
     1861 accaatgcga tccataaacg gcaccaccag actgagcccc ggctgtaacg ttttggtata
     1921 gcgaccaaaa cgttctaccg tccattgata gccctgcggc acgattttga cgcccgcgcc
     1981 gacaatgacc agcgcgacaa aaatgagaat cgggataaag ataagcatcg gaaaaacctc
     2041 ctgttgtacc gtccataatc agcaaaattg ctgcttgatt aaacaaatta tacctgatta
     2101 ctgaaagaga gttccccctt attcctgcga aggataaact gtttttagta aaaatcagaa
     2161 aaagggaaca gcgatgcagg aaaatagtcc tttgcttcag ctacaaaacg taggatatct
     2221 ggcgggtgat gcgaagattc ttaataacat caatttttcg ctgcgtgctg gcgaatttaa
     2281 gttaattacc ggtccttctg gttgtggcaa aagtacgctg ctaaaaatag ttgcttcatt
     2341 gatcagccca accagcggaa cgttactgtt tgaaggtgag gatgtcagca cactaaagcc
     2401 agaaatctac cgtcagcaag tctcttactg cgcccagacg ccgacgctgt ttggcgatac
     2461 ggtatacgat aatctgatct ttccctggca gatccgtaac cagcagcctg acccggccat
     2521 ttttctcgat tttcttgaac gcttcgcctt gccggacagc attttgacga agaatatcgc
     2581 cgagctatct ggtggtgaaa aacaacgcat ctcattgatt cgtaacctgc aatttatgcc
     2641 gaaggtttta ttgctggatg aaatcaccag tgcgctggat gaaagtaata aacataacgt
     2701 taatgagatg atccatcgtt atgtgcgcga gcaaaatatt gccgtgctgt gggtgacaca
     2761 cgataaagac gaaattaatc atgcggataa agtgattaca ctgcaaccgc atgccggaga
     2821 aatgcaggaa gcacgctatg aactcgcata atattactaa cgaatcatta gcactggcat
     2881 taatgctggt agtggtggca atcttaatta gccataaaga aaaactggcg ctggagaaag
     2941 atattctctg gagcgtcggg cgcgcgataa ttcagctgat tattgttggc tatgtgctga
     3001 agtatatttt cagcgtggat gatgccagcc tgacattatt gatggtgtta tttatctgct
     3061 ttaatgcggc gtggaacgcg caaaaacgca gtaaatatat tgctaaagct tttatctcat
     3121 cgtttattgc cattacggtc ggggcgggaa ttaccctggc ggtgctgatt ctctcagggt
     3181 cgattgaatt tatcccgatg caggtgatcc ctatcgccgg gatgattgcc ggtaacgcca
     3241 tggtagcggt ggggttgtgt tacaacaatt tagggcaacg ggtcattagc gaacagcaac
     3301 agattcagga gaaactgagt cttggcgcga cgccgaagca ggcttcagcg atattgattc
     3361 gcgacagtat tcgcgcggct ttaattccga cggtcgattc agcaaaaacg gttggcttag
     3421 tgagtttacc aggaatgatg tccgggctga tatttgccgg gattgatccg gtgaaggcga
     3481 ttaaatatca gattatggtg acctttatgc tgctctcaac cgccagcttg tcgaccataa
     3541 ttgcctgcta tttaacctat cgtaagtttt ataattcgcg ccaccagttg gtggtgacgc
     3601 aattgaagaa gaaatgatgc gatgccggat gcgcagcatc gcatccggcg ttgtggttca
     3661 tgtgccgatc aacggaatat caatacaaca atgcatacag ctggcggcga tacttcgacg
     3721 ccagtgcatc acccgtaccc agcgcagcga ggatctcctg gaacgtttta cgcgtctgac
     3781 cgtctgcggc ggtgagatct ttacgcagat gcccgaacag caactccagc gcctcttcat
     3841 tgcgcccaac ctgatgcagt tgcagcgcca gttgcgtcgc cagtgcggca tcttctggat
     3901 tctccgccac ctgctgttgc aactgttgaa tttccggcgt atcagccgcc tgcttcagca
     3961 gttcgatttg cgccaccagc ccctggtagc gggtgtcctg atcctgcaac ggaatggttt
     4021 tcagcaccgc ttccgcatct tcagaacggt tcagcgcaat cagcgtttct gccagcagca
     4081 ggccgatctc cccgttctga ttcgacaact gccaggcgtc tttcagcaat ggcagggcat
     4141 cggtgtaatt gccttcctgc atcagttgca tcgcctgctg cgctttcagc tcttcttcgc
     4201 gcggcagcac tttatccagc agggcgcgga tcgcctcttc cggttgcggc ccctggaagc
     4261 catctaccgg ttgcccgttc tggaacagat acacggtcgg aatcgcgcgc agaccaaact
     4321 gggcggcaat catctgctcc gcgtcgcagt ccagcttcgc cagaataaat tgcccgttgt
     4381 actgcgccgc aaggctttcc agaattgggg ttaactgcaa acagtgctgg ctacgttcag
     4441 accaaaaata gaacagcacc ggagtggtca tcgactgttc aagaacctgt tgcaggttag
     4501 attcgttaat gttgacaata ttttctacgg acatggagtc gctctctgtt gtcgatattt
     4561 tctttgacat gggggcttaa gcgcgcgctt caactcaccc ctgcaatatt ttgtccatca
     4621 cgcgccccgg cagcaggcgc ttaagcacca ttaccgccca ggtcaccagc gttaccggat
     4681 agcgcatctt cggcttctcg ctaataaaag catggcgtac tttgtccacc accgcttccg
     4741 gtcccaacgt aaagcgggcg gcgatgccgg gattttcgac tggtttatca ctttgcgtct
     4801 ggttgacgtt gtcggtgaag cgagtacgaa tgggaccggg ttcgatcagg ctgactttaa
     4861 ttccgctgcg gcgcagctcc atgcgcagcg cgtctgacca cgcctccagc gcatatttac
     4921 tggccgcgta agcgccacga cccggtgtgg agattaatcc catcaccgat gaggtcatca
     4981 caatacgccc ttcaccgtgc ggcaacatcg cgggtaacag gcgcatggtg agctggtgtg
     5041 cgccgaaaaa gttggcggaa aactgctgtt ccatctgcgc acggctgatg gtggaaaggg
     5101 ggccatacat gccgaatccg gcattgttaa agatcccata cagacaatta tcggtcaggg
     5161 cgatcacctc gtcggctgcg cgatcaacac tttctggtga atccagatcg atcaacacgc
     5221 cggtaaatcc catgctgttc atgcgctcaa catcatccgg tttccggcaa cctgccagca
     5281 catgaaaacc ctggcgtttt aattcgagcg cgctttccag gccaattcca ctggaacatc
     5341 cggtaattaa gaccgatttt tgcataactt tacctgtcag gatctccgtt gctttatgag
     5401 tcatgattta ctaaaggctg caactgcttc gccatccagt cggcaataaa cggctgggcg
     5461 tcgcggttgg gatgaatacc gtcatcctgc atccattgtg gcttgaggta gacctcttcc
     5521 attaaaaagg gcagcagcgg aacatcaaac tctttggcga gtttggggta aatggcgcta
     5581 aaggcttcat tataacggcg accatagttt gcaggcagac gtatttgcat taacaatggt
     5641 tcagcgttgg cggctttgac atcctgcaaa atctggcgca gcgtttgctc ggtttgctgt
     5701 ggctgaaaac cacgcaaacc gtcattgccg cccagttcaa ccagcaccca acgcggctga
     5761 tgctgtttca gcagagccgg aaggcgcgcc agcccttgtt gcgaggtgtc gccgctgatg
     5821 ctggcattga ctaccgacgt tttactctgc cacttatcat tcaacaaggc aggccaggcc
     5881 gcgctggcag acattcgata cccggcgctc aggctatcac ccagaatcaa taacgtgtcc
     5941 gctgcggcag cacggaaggt taacaggacc agaaacagga agggcaaatg ccagcggaaa
     6001 acattgttga agttcatcat cttaagaagt ccgtcggtca gggggagcat gaactctcca
     6061 tcctcaccgg agttgagctg gttgtcaaac gtggcgagac catcgcactg gtgggcgagt
     6121 cgggatcggg taagtcaacc ttgctggcga tcctcgccgg gctggatgac ggcagcagtg
     6181 gcgaagtgag tctggtggga caaccgctac ataatatgga cgaagaagcg cgggcaaagt
     6241 tgcgcgcgaa gcacgtcggc tttgtttttc agtcatttat gttaattcct acccttaacg
     6301 cgctggaaaa cgtcgagctt ccggcgctgc tgcgcggtga gagtagcgcg gaaagtcgta
     6361 acggagcgaa agcgttgctc gaacagttag ggctgggtaa acgtctggat catcttccgg
     6421 cacagctttc cggcggtgaa cagcaacgag tggcgctggc acgagccttt aacggtcgac
     6481 ctgatgtgct gtttgccgac gaacccaccg gtaaccttga ccgccagacg ggcgataaaa
     6541 ttgccgacct gctgttttcc ctcaaccgtg agcatggcac cacgttgatt atggtgaccc
     6601 acgacctgca actggcggca cgctgcgacc gctgcttacg gctggtgaac gggcagttgc
     6661 aggaggaagc atgattgcac gctggttctg gcgcgaatgg cgctcgccgt cgctattaat
     6721 tgtctggctg gcgctaagcc tggcggtggc ctgcgtgctg gcgctgggca atatcagcga
     6781 tcgcatggag aagggcttaa gccaacaaag ccgggagttt atggcgggcg atcgggcgtt
     6841 gcgcagttcg cgcgaagtgc cgcaagcgtg gctggaggaa gcgcaaaagc gcggcctgaa
     6901 agtcggcaag cagctgactt tcgccaccat gacatttgca ggcgacacac cgcagctggc
     6961 gaacgttaaa gcggtggatg atatctaccc gatgtatggc gatctgcaaa ccaatccccc
     7021 tggcctgaaa ccgcaggcgg gcagcgtatt gctggcccca cgcctgatgg cactgcttaa
     7081 cctgaaaacg ggcgacacca ttgacgtggg cgatgccacc ttgcggattg ccggagaagt
     7141 gattcaggaa ccggattccg gttttaaccc cttccagatg gctccgcgtc tgatgatgaa
     7201 tctggcggat gtcgataaaa ccggagccgt gcagccgggg agtcgggtca cctggcgcta
     7261 taaattcggc ggcagcgaga accagctcga cagctatgag aaatggttgt tacctcagct
     7321 taaacccgaa caacgctggt acggtctgga acaggacgaa ggcgcgctgg ggcgatcgat
     7381 ggaacgctcg caacagttcc tgctgctttc ggcacttctg accttgctgc tggcagtggc
     7441 agcagtggcg gtagcgatga atcattactg tcgcagtcgc tacgatctgg tggcgatcct
     7501 caaaacgctg ggggcagggc gagcgcaact gcgtaagcta atcgtcggtc agtggttgat
     7561 ggtgctgacg ctttcagccg ttaccggtgg ggcgataggc ctgttgttcg aaaacgtgtt
     7621 gatggtgctg ctcaagcccg ttctgcctgc tgcactaccg ccagccagcc tctggccatg
     7681 gctatgggcg ctgggcacca tgacggtcat ctcgctgctg gtggggctac gaccataccg
     7741 cttgttgctg gcaacgcagc ctttacgcgt attacgtaat tatgtggttg cgaacgtctg
     7801 gccgctgaag ttttatctgc cgattgtcag tgtggtggtt gtgctgctgc tcgccggatt
     7861 aatgggtggc agcatgctgc tttgggcggt gctggcgggc gcggtagtac tggctttgct
     7921 gtgcggtgta ctgggctgga tgctgctgaa tgtacttcgc cgcatgacgc tgaaatcgct
     7981 gcctctgcgc ctggcggtta gccgcctgtt acgtcagccg tggtcaacgt taagccagct
     8041 ttcggcattt tcgctctcct ttatgctgct ggcactgcta ctggtgctgc gtggcgatct
     8101 gctcgaccgc tggcagcagc agttaccgcc agaaagcccg aactactttt taatcaacat
     8161 cgccacagaa caggttacgc cgctaaaagc gttcctcgcg gaacatcaga tagtcccgga
     8221 atcgttttat ccggtggtgc gagcgcggct gacggcgatt aacggcaagc cgacagaagg
     8281 caatgaagat gaggcgctca accgcgaact caatcttacc tggcaaaata cgcggcccga
     8341 tcataatccg attgtcgccg gtaactggcc gccaaaagct gatgaagttt cgatggaaga
     8401 ggggctggcg aaacgcttaa acgttaccct cggcgatacc gtgactttta tgggcgatac
     8461 ccaggagttc cgcgctaaag tgaccagcct gcgcaaagtg gactgggaaa gtctgcggcc
     8521 taatttctat tttattttcc ctgaaggggc attagacggg caaccgcaga gctggcttac
     8581 cagtttccgc tgggagaatg gcaacggcat gttgacgcaa ctcaaccgcc agttccctac
     8641 cattagcctg ttagacattg gcgcgatttt aaaacaggtc ggtcaggtgc tggagcaggt
     8701 aagtcgggcg ctggaggtga tggtggtgct ggtcaccgcc tgcggtatgt tgctgttgct
     8761 ggcacaggtg caggtgggaa tgcgtcagcg tcatcaggag ctggtggtgt ggcgcacact
     8821 cggtgcgggg aaaaaactgc tgcgtaccac gttgtggtgt gagttcgcca tgcttgggtt
     8881 tgtttccggc ctggtggccg caattggtgc ggaaacggca ctggcagtgt tgcaggcgaa
     8941 agtgtttgat tttccgtggg agccagactg gcgattgtgg attgttctgc cgtgcagcgg
     9001 tgcgctgctg ctgtcgcttt tcggcggctg gctgggcgcg agactggtta agggtaaggc
     9061 gctgttcagg cagtttgcgg ggtgatgaga gtaaataagg ggcgttctgg ttgaatggaa
     9121 cgccttgttt aaattaattc tacaatatgt ttcattgatg atgttattga attggtgatt
     9181 tcctatcttt ctattgctga tattaatctg aatcggtgga gtttaataat tttaaatata
     9241 gccctacaaa aataactcag actaataaaa
//
LOCUS       DBFIFM010000175         9219 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.175, whole genome shotgun sequence.
ACCESSION   DBFIFM010000175 DBFIFM010000000
VERSION     DBFIFM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.175"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(50..373)
                     /locus_tag="N7C74_003727"
     CDS             complement(50..373)
                     /locus_tag="N7C74_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HGU2433520.1"
                     /translation="MKRTLLIAVWAIGLMSDSAMALTLNEARSQGRVGETLNGYLVAL
                     QTDAETQALVKDINEARNHSYQQLAKQNNVSTKEIAKLAGQKLVARAKSGQYVQGVNG
                     KWLRK"
     gene            complement(381..566)
                     /gene="ynbE"
                     /locus_tag="N7C74_003728"
     CDS             complement(381..566)
                     /gene="ynbE"
                     /locus_tag="N7C74_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HGU2433521.1"
                     /translation="MKIVIAAFASSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEDLLKTRSDLF"
     gene            complement(563..3202)
                     /gene="ydbH"
                     /locus_tag="N7C74_003729"
     CDS             complement(563..3202)
                     /gene="ydbH"
                     /locus_tag="N7C74_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HGU2433522.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPSVKLVGEFTMPLMPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANAGPMTLRLDKDTADAVAKDNMTAGTAINWLRYMEIS
                     RSSTRINLDNLGVLTMQANISGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQV
                     WLEQNAQLPETGCPSGKECEEKQ"
     gene            3410..4399
                     /gene="ldhA"
                     /locus_tag="N7C74_003730"
     CDS             3410..4399
                     /gene="ldhA"
                     /locus_tag="N7C74_003730"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HGU2433523.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4510..4932
                     /gene="hslJ"
                     /locus_tag="N7C74_003731"
     CDS             4510..4932
                     /gene="hslJ"
                     /locus_tag="N7C74_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HGU2433524.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTCDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4929..5195)
                     /gene="ydbJ"
                     /locus_tag="N7C74_003732"
     CDS             complement(4929..5195)
                     /gene="ydbJ"
                     /locus_tag="N7C74_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HGU2433525.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5469..8993
                     /gene="nifJ"
                     /locus_tag="N7C74_003733"
     CDS             5469..8993
                     /gene="nifJ"
                     /locus_tag="N7C74_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HGU2433526.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPAFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         2297 a         2413 c         2258 g         2251 t
ORIGIN      
        1 ctgtaaaaga tcattacatt aatagataca ttttgtagcc aactataatt tatttgcgca
       61 accatttacc attaactccc tgaacatatt gccccgactt cgcgcgagca acaagctttt
      121 gtccagccag tttggctatc tctttagtgg atacattatt ttgctttgcc agttgttggt
      181 aactatggtt acgagcttca ttaatatctt ttactaatgc ctgtgtttcg gcatccgttt
      241 gtagcgccac caggtaaccg ttaagcgttt cacctacccg cccctgactt ctggcttcgt
      301 tcagggttaa agccattgca ctgtcgctca tcaggccaat ggcccataca gctatgagta
      361 atgttctttt catcatcacc tcaaaataga tcgctacggg ttttgagcag gtcttcaacg
      421 tccttatccg ctttaatgat gatttcatgc tcaattttaa cgttcatatt aatggtgatt
      481 ggctctttcg gcgcagccac ttcaatgcga ggcgtgcaac caaccaacat aaaagaggac
      541 gcaaacgcgg ctatcactat tttcattgtt tttcctcaca ctcttttcct gatgggcagc
      601 cagtttccgg tagctgggca ttctgctcca gccatacctg gagattatcg ccaaagcgta
      661 aactgcgcca taacgtaaaa atattctctt catggtgata attaaggttt accgtaccgc
      721 ttttaccatc gacgcgactg gtgcccgaaa tgttggcctg catggttaac acaccgagat
      781 tatctaaatt aatccttgtt gatgagcgac tgatttccat ataacgcaac cagttgatgg
      841 cagttccagc ggtcatattg tctttggcca ccgcatctgc agtgtcttta tccagacgca
      901 gcgtcatcgg ccccgcgttt gccaaccagc catctttgac aatccatttt tcattgttca
      961 gccatagtgg caacgcccca ctaaatgccc cagacatagc aaactgtttc ggattaaccg
     1021 cgctaaccag ttcgctggat gagagattat tcagccgcaa cagcgccggg tcatgttgcg
     1081 gcatacgtaa ttgtttcatc agcacgttac cgcctaacac atcgacgcta acatcagtca
     1141 acagcaatgg ttcttcttca gtccacggat aacgcccttg caaatcagcc gtaatatttt
     1201 tcgctgtcac cagattaatc acttcggcaa ttcgcaacgt aacggggccg cgagtcccca
     1261 gatgccaggc tccatcggca aaacggaaag gcaggacaaa atcgacaccg ttaacctgat
     1321 tatctggcat ccaggcacta ccgcctttca acacgccgtg ccctcccgcg cggaatcctt
     1381 gttcaggcgc agcagaaaat gcaacctgag catacagttc accatcgcgt aagttcatct
     1441 tccagtcggg tggcaccagc ggctggaata cagtcagtga ttgtttaggc caccaggcgt
     1501 taccgcgcag acgaataccg tcccagcgac catttacccg aaccgggcca atctcaccag
     1561 catgtaaatc gcctttaaag aggaaatacg tcggatcgcg cccatcgacg ctaaatttta
     1621 aggttgatgg cggtaacaca ctgccgccag tgaacagcgt ttgcccggcg tccagtgaca
     1681 gcgcgccgct gaaggaggga tgctgtgcgt cacgtaccca gacgacgggc ttgtcgagaa
     1741 ttaatcgcgg cttttctacc gtcatcgtac cgtattgtaa ctggtcgaaa ccggtggaga
     1801 gatcggtcag cgtaatcgtg ctgtcatgcc actcaccttt tcctgcgaca tcccaggtgg
     1861 cattcatcgg tgtaaaactc ccttttcccc agtagcgcca ctgccagcgg ccagcgtcag
     1921 ggagaaaatc attcgccagc ccatccatat gcagcacgaa atcgcccagt tcattttcat
     1981 gcgcctgcaa aatggcctgc aaacgtccgt caacaccacg ttgggtgact tttacacccg
     2041 ctaaaggcca gcggatttca tcgatgtcca gcgaatcgat gactcttccc tttgaacgaa
     2101 gtaacgcgcc tggctcaaag gtcagcgttg ggtcagacag acttccactt agctgcgcag
     2161 gtaaacgggc atataaaatg aggtccgcct gtttcgcttc accggtcagc tgcagaggca
     2221 gctggctgtt atccatgctt aattttcctg ggccaaaatt aagcaccgcg ttgcccttac
     2281 ccgcttgccc ctgggtcagc acactcagtc gtccgctgac cagagcgttc tcaagccctg
     2341 cctgccagtt gtcgactttg acacccagtc ggccactcaa aggaaaacct gcatacggcc
     2401 agctccagcg accatcgctt acggtcaatt gttgacgagt aatttgccac ggcaaatcga
     2461 gcaacggatc gccgttatcc cgtgccagca cgatcaattg cccgctattt tcctgccagt
     2521 ccagctcggc atccaccagt gacggttcct gcggcaagtt taacgtcgca gtagcatgcc
     2581 cacttacagg aagtccatcc ggcatgagcg gcatagtaaa ttcccccacc agtttaaccg
     2641 acggctgatt ttcaaacgcg acgacatcca gttcgctgac tgtaagttgt tgccctttca
     2701 gctggccttg aaatttaact ttttcgccct gataacgcag ttgctggata tcagaggtta
     2761 atgcgagaga gagttttccc tgccattcct gccagggaga aaaaatcagt ttatcaatat
     2821 tgatccaggt gttaggcagc atggactgcc attgcgcgag ggtttttgga gcagctggcg
     2881 attgctccgt ctgcggcaat ttcgccagac aagcagaatc aagttctacc gtgccgacgt
     2941 tcaataacca gcggctggga tgtgaaaggc tggcgttggt gatatgcgca agctgacaat
     3001 ctcccaccag ataacggaga tcgggaatga ttaaaccttt acgcgtaatg cgtgggcttt
     3061 catctaatgc aatacgtgtc ccgagcggta gccagatgcc cgccagcgtg ggaacccaca
     3121 gcccgagcgt catcagcagc gtcaacggca caagaataat cagtaataac agcgcgagaa
     3181 cggctttata tttacccagc atgggtagtt aatatcctga tttagcgaaa aattaagcat
     3241 tcaatacggg tattgtggca tgtttaaccg ttcagttgaa ggttgcgcct acactaagca
     3301 tagttgttga tgaatttttc aatatcgcca tagctttcaa ttaaatttga aattttgtaa
     3361 aatattttta gtaacttaaa tgtgattcaa catcactgga gaaagtctta tgaaactcgc
     3421 cgtttatagc acaaaacagt acgacaagaa gtacctgcaa caggtgaacg agtcctttgg
     3481 ctttgagctg gaattttttg actttctgct gacggaaaaa accgctaaaa ctgccaatgg
     3541 ctgcgaagcg gtatgtattt tcgtaaacga tgacggcagc cgcccggtgc tggaagagct
     3601 gaaaaagcac ggcgttaaat atatcgccct gcgctgtgcc ggtttcaata acgtcgacct
     3661 tgacgcggca aaagaactgg ggctgaaagt agtccgtgtt ccagcctatg atccagaggc
     3721 cgttgctgaa cacgccatcg gtatgatgat gacgctgaac cgccgtattc accgcgcgta
     3781 tcagcgtacc cgtgacgcta acttctctct ggaaggtctg accggcttta ctatgtatgg
     3841 caaaacggca ggcgttatcg gtaccggtaa aatcggtgtg gcgatgctgc gcattctgaa
     3901 aggttttggt atgcgtctgc tggcgttcga tccgtatcca agtgcagcgg cgctggaact
     3961 cggtgtggag tatgtcgatc tgccaaccct gttctctgaa tcagacgtta tctctctgca
     4021 ctgcccgctg acaccggaaa actaccatct gttgaacgaa gccgccttcg atcagatgaa
     4081 aaatggcgtg atgatcgtca ataccagtcg cggtgcattg attgattctc aggcagcaat
     4141 tgaagcgctg aaaaatcaga aaattggttc gttgggtatg gacgtgtatg agaacgaacg
     4201 cgatctgttc tttgaagata aatccaacga cgtgatccag gatgacgtat tccgtcgcct
     4261 gtctgcctgc cacaacgtgc tgtttaccgg gcaccaggca ttcctgacag cagaagctct
     4321 gaccagtatt tctcagacta cgctgcaaaa cttaagcaat ctggaaaaag gtgaaacctg
     4381 cccgaacgaa ctggtttaat cttgccgctc ccctgcaatc caggggagct gattcagata
     4441 atccccaatg acctttcatt ctctattctt aaaatagtcc tgagtcagaa actgtaattg
     4501 agaaccacaa tgaagaaagt agccgcgttt gttgcgctaa gcctgctgat ggcgggatgt
     4561 gtaagtaatg acaaaattgc tgttacgcca gaacagctac agcatcatcg ctttgtgctg
     4621 gaaagcgtaa acggtaagcc cgtgacctgc gataaaaatc cgccagaaat cagctttggt
     4681 gaaaaaatga tgatttccgg cagcatgtgt aaccgcttta gcggtgaagg caaactgtct
     4741 aatggtgaac tgacagccaa agggctggca atgacccgta tgatgtgcgc taacccgcag
     4801 cttaatgaac tcgataacac cattagcgaa atgctgaaag aaggtgcaca agtggatctg
     4861 accgcgaacc agttaacgct ggcgaccgcg aaacagacat taacttataa gctggcggat
     4921 ttaatgaatt aatagctgcc acagctcccg gcggcaagtg actgttcgct acagcgtttg
     4981 ccgttgggta atgcacacat cccaatcgcc gtaccatcca gttgacgggc aacagaaagc
     5041 gaaccgccga tcattgcaca atttgcttct ccactactgg acatcgacgc ttttaaacct
     5101 ggcgctacgt gcgccgcagt cgcctgctga acaggctcac tactacacgc cgacaacaat
     5161 aaagcggcac accctaccca aaacgctgct cgcatctctt cttcctctga tcttcaagcc
     5221 aaacgacacc gccataaata ataggcagca cagagggcga cgtcgagagc tgtcctgcgc
     5281 gttgccccgc atttttactt ttttatggct atttttttgc cctctgtttg atcaaaacat
     5341 tcattacgct gatgtggggg acacaaaagc gaaaatgcag aagaaagcca tttgctaaaa
     5401 ttgaaagatt actactgggc gcgcagcaat ttcgtgcgcc cctcatttgc gcaatgtaag
     5461 ggtgtcatat gattactatt gacggtaatg gcgcggttgc ttcggtggcg tttcgcacca
     5521 gtgaagttat cgccatctac cctattaccc ccagttccac gatggcagaa caggctgatg
     5581 cctgggccgg aaacggcttg aagaacgttt ggggagacac accacgcgtg gttgaaatgc
     5641 agtcggaagc gggtgctatc gctaccgtgc atggcgcttt gcagacgggt gccctttcaa
     5701 catcgtttac gtcatcgcag ggtttgctgc tgatgatccc gacgctgtac aaactggcag
     5761 gcgaactcac accgtttgtc ctgcatgtag cggcacgtac cgttgccaca catgcactct
     5821 ctatttttgg cgatcattcc gacgtcatgg cggtgcgcca gacgggttgc gcgatgttgt
     5881 gtgcggcaaa cgtccaggaa gcgcaagact ttgctctcat ttcgcacatc gcgacgctga
     5941 aaagccgcgt gccatttatt catttctttg atggtttccg tacgtcccac gaaatcaata
     6001 aaattgtccc gctggccgat gacactattc ttgatctcat gccgcaggcc gaaattgatg
     6061 ctcatcgcgc ccgggcgctc aacccggaac atcccgtgat ccgcggtacg tcagccaatc
     6121 ctgacactta tttccagtct cgcgaagcca ccaacccatg gtacaacgcg gtctatgacc
     6181 atgttgaaca ggcgatgaat gatttctctg ccgcgacagg tcgtcagtat cagccgtttg
     6241 aatattacgg gcatccgcaa gcggaacggg tgattatcct gatgggctct gccattggca
     6301 cctgtgaaga agtggttgat gaattgctaa cccgtggcga aaaagttggc gtgctgaaag
     6361 ttcgcctgta ccgtcccttc tccgccaaac acttgctgca agctctgccg ggatccgtac
     6421 gcagcgtggc ggtactggac agaaccaaag aacccggtgc ccaggcagaa ccgctctatc
     6481 tggatgtaat gaccgcactg gcagaagcct ttaataatgg cgagcgcgaa actctgcccc
     6541 gtgtcattgg tgggcgctat ggtctttcat ccaaagaatt tggcccggac tgtgtactgg
     6601 cggtatttgc cgagctcaac gcggctaaac cgaaagcgcg ctttacggtt ggtatttacg
     6661 atgatgtgac caatctgtca ctgccgttgc cggaaaacac cctgccaaac tcggcgaaac
     6721 tggaagcctt gttttatggc cttggtagtg atggcagcgt ttccgcgacc aaaaacaata
     6781 tcaagattat cggtaattcc acgccgtggt acgcacaggg ctattttgtt tacgactcca
     6841 aaaaggcggg cggcctgacg gtttctcacc ttcgcgtgag cgaacagccg attcgttccg
     6901 cttatctcat ttcccaggct gattttgttg gctgccacca gttgcagttt atcgataaat
     6961 atcagatggc tgagcgttta aaacctggcg gcattttcct gctcaacacg ccgtacagcg
     7021 cagatgaagt gtggtcgcgc ttgccgcagg aagttcaggc cgtgctaaat cagaaaaaag
     7081 cgcgcttcta tgtgattaac gcggcgaaaa tcgcccgcga atgtggcctg gcggcccgta
     7141 ttaataccgt catgcagatg gcttttttcc atctgacaca aattctgcct ggcgatagcg
     7201 ccctcgcaga attgcagggt gcgattgcca aaagttacag tagcaaaggt caggatctgg
     7261 tggaacgcaa ctggcaggct ctggcgctgg cgcgtgaatc cgtagaagaa gttccgttgc
     7321 aaccggtaaa tccgcacagc gccaatcgac cgccagtggt ttccgatgcc gcccctgctt
     7381 tcgtgaaaac cgtaaccgct gcgatgctcg ccgggcttgg tgatgccctc cccgtttcgg
     7441 cgctgccgcc agacggcacc tggccgatgg gcaccactcg ctgggaaaaa cgcaatatcg
     7501 ccgaagagat ccccatctgg aaagaggaac tctgtaccca atgtaaccac tgcgtcgccg
     7561 cttgcccaca ctcagctatt cgcgcaaaag tggtaccgcc tgaagcgatg gaaaacgccc
     7621 ctgccagcct gcattcgctg gatgtgaaat cgcgtgatat gcgcgggcag aaatatgtct
     7681 tgcaggtggc tccggaagat tgcaccggtt gtaacctgtg cgtcgaagtt tgcccggcga
     7741 aagaccgtca gaatccagag attaaagcca tcaatatgat gtcgcgcctg gaacatgtcg
     7801 aagaagagaa aatcaattac gatttcttcc tcaacctgcc agaaatcgac cgtagcaaac
     7861 tggaacgtat tgatattcgc acatcgcagc tgattacacc gctgtttgaa tattccggtg
     7921 cttgctccgg ttgtggcgag acgccgtata ttaaattgct gactcagctc tatggcgacc
     7981 ggatgttgat cgctaacgcc accggctgtt cttcaattta tggcggtaac ctgccctcta
     8041 caccgtatac caccgatgcc aacggtcgtg ggccggcatg ggcgaactct ctatttgaag
     8101 ataatgccga atttggcctt ggtttccgcc tgacggtcga tcaacaccgt gtccgcgtgc
     8161 tgcgtctgct ggatcaattt gccgataaaa tcccggcgga attactgacg gcgttgaaat
     8221 cagacgccac gccagaggtt cgtcgtgaac aggttgcagc tttacgccag caactcaacg
     8281 atgttgccga agcacatgaa ctgctgcgtg acgcagatgc actggtggaa aaatcaatct
     8341 ggctgattgg tggcgacggc tgggcttacg atatcggctt tggcggtctg gatcatgtat
     8401 tgagtttgac ggaaaacgtc aacattctgg tgctggatac gcaatgctat tccaacaccg
     8461 gtggtcaggc gtcgaaagcg acaccgctgg gtgcagtaac taaatttggc gagcacggca
     8521 aacgtaaagc gcgtaaagat cttggcgtca gtatgatgat gtacggtcat gtttatgtgg
     8581 ctcagatttc tctcggcgcg cagctgaacc agacggtgaa agcgattcag gaagcggaag
     8641 cgtatccggg accatcgctg atcattgctt atagcccgtg tgaagagcat ggttacgatc
     8701 tggcactcag ccatgaccag atgcgccaac tcacagctac cggcttctgg ccgctatatc
     8761 gcttcgatcc gcgtcgtgcc gatgaaggca aactgccgct ggcgctggat tcacgcccgc
     8821 cgtcagaagc actggaagaa acgttacttc acgagcaacg tttccgtcgt ctgaattcgc
     8881 agcagccaga agtggcagaa cagttatgga aagatgctgc agctgatttg caaaaacgct
     8941 atgacttcct ggcacaaatg gccggaaaag cggaaaaaag caacaccgat taaatgctct
     9001 ggataaggat tatccaattc taaaaaaaag cccggacgac tgttcgggct tgtcttttta
     9061 tatgttggaa aatcagtggc aatgcaatgg cccaacagaa aataaaatct gtgacaataa
     9121 aggcatataa cccgcgcaga ataacgtata gaaaattaaa agtataattt ttattttgta
     9181 tgtattatct tcgatacatt caccagactt attatcatt
//
LOCUS       DBFIFM010000176         9152 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.176, whole genome shotgun sequence.
ACCESSION   DBFIFM010000176 DBFIFM010000000
VERSION     DBFIFM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.176"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            202..1833
                     /gene="oppA"
                     /locus_tag="N7C74_003734"
     CDS             202..1833
                     /gene="oppA"
                     /locus_tag="N7C74_003734"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HGU2433527.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            1919..2839
                     /gene="oppB"
                     /locus_tag="N7C74_003735"
     CDS             1919..2839
                     /gene="oppB"
                     /locus_tag="N7C74_003735"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HGU2433528.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            2854..3762
                     /gene="oppC"
                     /locus_tag="N7C74_003736"
     CDS             2854..3762
                     /gene="oppC"
                     /locus_tag="N7C74_003736"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HGU2433529.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3774..4787
                     /gene="oppD"
                     /locus_tag="N7C74_003737"
     CDS             3774..4787
                     /gene="oppD"
                     /locus_tag="N7C74_003737"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HGU2433530.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4784..5788
                     /gene="oppF"
                     /locus_tag="N7C74_003738"
     CDS             4784..5788
                     /gene="oppF"
                     /locus_tag="N7C74_003738"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HGU2433531.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(5841..6170)
                     /locus_tag="N7C74_003739"
     CDS             complement(5841..6170)
                     /locus_tag="N7C74_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HGU2433532.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6205..7665)
                     /gene="cls"
                     /locus_tag="N7C74_003740"
     CDS             complement(6205..7665)
                     /gene="cls"
                     /locus_tag="N7C74_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HGU2433533.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFHSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKVYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7808..7981
                     /locus_tag="N7C74_003741"
     CDS             7808..7981
                     /locus_tag="N7C74_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HGU2433534.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8036..>9152)
                     /gene="kch"
                     /locus_tag="N7C74_003742"
     CDS             complement(8036..>9152)
                     /gene="kch"
                     /locus_tag="N7C74_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HGU2433535.1"
                     /translation="DIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWA
                     ISIILLLIALIYTLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFT
                     TLLFYSTYGALYLSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVI
                     ISGITVFATSMTSIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQ
                     RGQNVTVISNLPEDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDND
                     ADNAFVVLSAKDMSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGE
                     EINNDMLVSMLLNSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2358 a         2175 c         2307 g         2312 t
ORIGIN      
        1 atgtgtctcg acaggggaga cacagtacga atcgacataa ggtgatcgtc tgaatcacca
       61 gaataaataa agtcggtgat agcaatccgt aacgataaag taacctgaca gcagaaagtc
      121 tccgagcctg tgcagggtcc caatccggga ttacacatgc tggttaatac cagtaattat
      181 aatgagggag tccaaaaaac aatgaccaac atcaccaaga gaagtttagt agcagctggc
      241 gttctggctg cgctaatggc agggaatgtc gcgctggcag ctgatgtacc cgcaggcgtc
      301 acactggcgg aaaaacaaac gctggtacgt aacaatggtt cagaagttca gtcattagat
      361 ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc gagacctgtt tgaaggctta
      421 ctggtcagcg atcttgacgg ccatccggca cctggcgtcg ctgaatcctg ggataataaa
      481 gacgcgaaag tctggacctt ccatttgcgt aaagatgcga aatggtctga tggcacgcca
      541 gtcacagcac aagactttgt gtatagctgg caacgttctg ttgatccgaa caccgcttct
      601 ccgtatgcca gttatctgca atatgggcat atcgccggta ttgatgaaat tcttgaaggg
      661 aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg atcacacatt agaagtcacc
      721 ttaagtgaac ccgttccgta cttctataaa ttacttgttc acccatcaac ttcaccggtg
      781 ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc agcctggtaa tatcgtcacc
      841 aacggtgcct ataccttaaa agattgggtc gtaaacgaac gaatcgttct tgaacgcagc
      901 ccaacctact ggaacaacgc gaaaaccgtt attaaccagg taacctattt gcccattgct
      961 tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac ttataacaac
     1021 atgccgatcg aattgttcca gaagctgaaa aaagagatcc cggacgaagt tcacgttgat
     1081 ccatacctgt gcacttatta ttacgaaatt aacaaccaga aaccgccatt caacgatgtg
     1141 cgtgtgcgta ccgcactgaa actgggtatg gaccgcgata tcattgttaa taaagtgaaa
     1201 gcgcagggcg acatgcccgc ctatggttac actccaccgt atactgatgg cgcaaaattg
     1261 actcagccgg aatggtttgg ctggagccag gaaaaacgta acgaagaagc gaaaaaactg
     1321 ctggctgaag cgggttatac cgcagacaaa ccgttgacca tcaacctgtt gtataacacc
     1381 tccgatctgc ataaaaagct ggcgattgct gcctcttcat tgtggaagaa aaacattggt
     1441 gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc tcgacacccg tcaccagggt
     1501 acttttgatg tggcccgtgc aggctggtgt gctgactaca acgaaccaac ttccttcctg
     1561 aacaccatgc tgtcgaacag ctcgatgaat accgcgcatt ataagagccc ggcctttgac
     1621 agcattatgg cggaaacgct gaaagtgact gacgaggcgc agcgcacagc tctgtacact
     1681 aaagcagaac aacagctgga taaggattcg gccattgttc ctgtttatta ctacgtgaat
     1741 gcgcgtctgg tgaaaccgtg ggttggtggt tataccggca aagatccgct ggataatacc
     1801 tacacccgga atatgtacat tgtgaagcac tgatggcaat acgtggggca ggagtgtcct
     1861 gctccacggt gtctgatttt tatcgcatta cagaaggcac aggccagaag gtagggcaat
     1921 gttaaaattt attctacgtc gctgtctgga agcgattccg acgctattta ttcttattac
     1981 tatttcgttc tttatgatgc gcctcgcgcc gggaagccct tttaccggcg aacgtacttt
     2041 accgccggaa gtaatggcca atatcgaagc gaaatatcat cttaatgatc cgatcatgac
     2101 acagtatttc agctacctga aacaactggc gcatggcgat ttcggtccat cgtttaaata
     2161 taaagattat tcggtcaatg acctggttgc atccagtttt cccgtttctg ccaaactggg
     2221 agccgcagca tttttccttg cggtaatact gggtgttagt gctggcgtta ttgccgcatt
     2281 aaaacaaaac accaaatggg actataccgt gatggggctg gcaatgaccg gggttgttat
     2341 ccccagtttt gtggttgcgc cattattagt catgatattt gcgatcattt tgcattggct
     2401 gccgggcggt ggctggaatg gtggggcgct taaattcatg atattgccga tggtggcgtt
     2461 gtcactcgcc tatatcgcca gtattgcgcg tattacccgt ggctctatga ttgaagtatt
     2521 acactccaac tttattcgta ctgcccgggc gaaagggtta cctatgcggc ggatcatttt
     2581 acgccacgca ttaaaacctg ctctgttacc cgtgctctcc tatatgggcc ccgcatttgt
     2641 cggcattatt accggttcta tggttatcga aaccatttat ggtttgccgg ggattgggca
     2701 actgttcgtt aatggcgcat tgaaccgtga ctattcctta gtgttaagcc tgaccatcct
     2761 ggttggtgcg ttaaccattt tgtttaatgc cattgtcgat gtgctatatg cggttatcga
     2821 cccgaaaatc cgttactgat actggagctc gcgatgatgt taagtaagaa aaacagcgag
     2881 acgctggaaa atttcagtga aaagctggag gtcgaagggc gcagcttgtg gcaggacgca
     2941 cgtcgacgtt ttatgcataa ccgtgcggcg gttgcaagtc tgatagtgct ggtgctgatc
     3001 gcgttatttg taatcctggc accgatgctt tcgcagtttg cctatgacga tactgactgg
     3061 gcgatgatgt ccagcgcccc ggatatggag tccggtcact actttggtac tgactcatcc
     3121 ggtcgcgacc tgcttgtgcg cgttgcgatt ggcggacgta tctcactcat ggtcggtgtt
     3181 gctgcggcac tggtggcagt ggtcgtgggg acactttatg gttcgctttc cggttatctg
     3241 ggcggtaaag tggattcggt aatgatgcgt ttgctggaaa tcctcaactc cttcccattc
     3301 atgttcttcg tcattttgct ggtgaccttt ttcggtcaaa acatcctgct gattttcgtg
     3361 gcgataggca tggtttcctg gctggatatg gctcgtattg tgcgtgggca aaccctgagt
     3421 ctgaagcgca aagagtttat tgaggcggca caagttggcg gtgtatcgac gtcgggcatt
     3481 gttattcgcc acattgtgcc gaacgtactc ggtgtggtgg tggtctacgc atcgctactg
     3541 gtgcccagca tgatcctctt tgaatctttc cttagcttcc tggggttggg tacgcaagag
     3601 ccgttaagca gctggggggc attgctgagt gatggcgcga actcaatgga agtctctcca
     3661 tggttactgt tgttcccagc gggattcctc gtggtgacgc tgttttgttt caactttatc
     3721 ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca gccatgagcg
     3781 taattgaaac tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg ctgaacgtga
     3841 aagatttgcg tgtcaccttt agtaccccgg acggcgacgt cacggcggtc aatgatttaa
     3901 atttttccct acgtgccgga gaaacgctgg gcattgtagg tgagtctggt tcgggtaaat
     3961 cgcaaactgc atttgcgttg atgggcctgt tggctgccaa cggacgcatt ggcggatcgg
     4021 caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg tgaactcaat aaactgcgcg
     4081 ctgaacaaat ctcaatgatt tttcaggacc caatgacttc gttgaatccc tatatgcgcg
     4141 tcggtgagca gttgatggaa gtgctgatgc tgcataagaa catgagcaaa gctgaggcgt
     4201 ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt aaacgcatga
     4261 aaatgtaccc gcacgaattt tctggcggca tgcgtcagcg agttatgatt gcgatggcat
     4321 tgctatgtcg acctaagctg ctgattgcgg atgaacccac tactgcgctg gacgtcaccg
     4381 tacaggcgca gatcatgacg ctattgaatg aactgaagcg ggaatttaat accgccatca
     4441 ttatgatcac ccacgatctt ggcgtggtgg cggggatctg cgacaaagtg ctggtaatgt
     4501 acgccgggcg cacgatggaa tatggcaacg cgcgcgatgt cttttatcaa cccgttcatc
     4561 cttattctat cggtttgctc aacgcggtgc ctcgtctcga tgcggaaggt gaaacaatgt
     4621 tgactattcc gggtaatccg ccaaacctgc tgcgattacc gaaaggttgc ccattccagc
     4681 cacgttgtcc gcatgcgatg gaaatttgta gtagcgcacc gccgctggaa gagtttacgc
     4741 ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact gttatgaatg ctgtaaccga
     4801 aggaagaaaa gtcctcctcg aaatcgccga tctgaaagtg cactttgaaa tcaaagatgg
     4861 caaacagtgg ttctggcaac cgccgaaaac gctcaaagcc gtagatggtg taactcttcg
     4921 cctgtatgaa ggggaaacat taggtgtggt aggggaatcg ggatgcggta agtccacctt
     4981 tgctcgcgcc atcattggcc tggttaaagc gaccgacggt catgttgcct ggttaggtaa
     5041 agagttgctg ggcatgaaac ccgatgaatg gcgtgccgtt cgcagtgata ttcagatgat
     5101 tttccaggat ccgttggcat cgctaaaccc gcgtatgacc atcggcgaga tcatcgctga
     5161 accactgcgt acttatcatc cgaaaatgtc acgccaggaa gttcgcgagc gcgtgaaggc
     5221 gatgatgctg aaagtcgggt tattgcctaa cctgattaac cgctatccgc atgagttctc
     5281 tggtgggcag tgccagcgta tcgggattgc gcgtgctctt attcttgaac cgaagctgat
     5341 tatctgtgat gagccggtgt cggcgctgga cgtgtcaatt caggcacagg tggtcaacct
     5401 gctccagcaa ttgcaacgtg agatgggatt gtcattaatt tttatcgctc atgacctggc
     5461 cgtggtaaaa cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg cggtagaact
     5521 ggggacctat gatgaggtct accacaatcc actacatcct tacaccaggg cattgatgtc
     5581 ggcagtcccc atacctgatc cggatctgga gaagaacaaa accatccagt tactggaagg
     5641 ggaattaccg tcgccgatca acccgccttc cggttgtgtt ttccgtaccc gttgcccgat
     5701 tgccggtccg gagtgtgcca aaacacgtcc tgtgctggag gggagtttca gacacgccgt
     5761 ttcttgcctg aaagtcgatc cgctttaaac acaaaaaggg ctgacagctg tcagcccttt
     5821 ttcacgccga aagtgattat ttattcccgc cagatgatat ggcaaagttt gtgatctttt
     5881 tcgcggcata acagaatgcg ggcgaaaaca tcgttgattt caccatcttc actgtccgcc
     5941 agaccaatca caacttcggc aaaaaagtct gggttcaaat cgaaatcaac atgttcctgc
     6001 cagtcttccg ccggatcgaa taactccgcg ccgccgcgct cttcaaactg aagattgaac
     6061 agcagaacat cagctggatc gagattgtcg gcagccagtt cgagaaaaat atcgtaggcc
     6121 tgctcaagcg tttcatcttc agtcaggcga ttgtttagat ccatatccat agtcactacc
     6181 tgtttaacct ctgttggcga cgttttacag caacgggctg aagaagtaaa acagtcgctc
     6241 ggcgacacgt tgccataatg gacgttttag ccataaacgg gcatcgagca gacgtgaacg
     6301 cgaaatataa tcgtcctgaa cggcggcaag gtctgcacca aaacctttat cgtcgattgc
     6361 cagggtaatc tcgaaattca gccacagact acgcatatca aggttaacgg tgccaaccag
     6421 acttagttcg ccatcgacca gcacgctctt ggtatgcagt aacccgcctt caaactgata
     6481 aactttaacc ccagcagcaa gcagttccgt aaagaatgcg cgactggccc agccgaccag
     6541 catcgagtca ttttttcgcg gaaggataat actgacatcc accccgcgct gcgccgccgt
     6601 gcaaatcgca tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca tgatcaaata
     6661 ttcacgcgcc gaataagccg cagtcaataa tgcctggtga atgagatctt ccggaaagcc
     6721 ggggccagaa gcaattgtgt gaatggtatg accgctggcc tgctcaaacg gcataatatt
     6781 gacatcaggt ggtggcggta gaatacgttt ccctgtttca atctcccagt cgcaggaata
     6841 aataatcccc atcgcggtgg cgatggggcc ttccatacgc gccatcagat caatccattg
     6901 ccctacgccc gcatcttgtt tgaagtagcg agggtcgacc atattcatgc tgccggtgta
     6961 cgcgatgtaa ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca tacggcgtaa
     7021 aaacacacgc atcagattga cctttaaggc ttcgaccact tcaataccgg cattacgcat
     7081 tagctcgggc cacgggctgt ggaaaaaagc tacactcccg gcggagtcga gcatcaatcg
     7141 gcaatgaatg ccgcgtcgcg cagccgccat taatgattca gccacctggt ccgccatgcc
     7201 gccgggctgc cagatataaa acaccatctc aatattatgg cgcgcgagct ggatgtcgcg
     7261 gattaacgcc tgcatcacat catctgactc ggtcatcagt tgtagctgat tccctttgac
     7321 cccagcgatc ccctgacgac gctcgcaaag cttgaataat ggcgcagcga cactgctatt
     7381 ttcttcggca aagatatgct tacaggcttt aaggtcgtta agccattttg cggtggaagg
     7441 ccacatcgct ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc caacggcaag
     7501 ataggcaata attccgacta acggcagaat gtaaataatc aacagccagg ccatcgcgga
     7561 gggaactgcg cgtcgtttca ttagaatgcg taaagttacg cctgcaatga gcaaccagta
     7621 tcccagaatg gccaaccaac tcaccaacgt ataaacggtt gtcatagatt agaaatcctt
     7681 ttgaaagcgc attgttatga gtttacgcat atggattcat ctggcaaata aaaacgcggg
     7741 aaaagcactg gtcagcgtcg ggggcggggg tataatgacc attctgttat tgcatagagt
     7801 agttaacatg aagcggagta gaacggaagt ggggcgctgg cgcatgcagc gtcaggctag
     7861 ccgacgtaaa tcgcgttggc ttgaggggca gtcgcgccga aatatgcgta tccacagcat
     7921 caggaagtgc attctaaaca aacagcgtaa ctcgttattg tttgcgatct acaatatcta
     7981 aatgtactgg gcaccattgc ggtgcccaat aagtttactc ttccgtcttt aaactctatt
     8041 tttgcgccga ttctttactg tcagctttcg tttcttgttc atcgttatcg ctgaaaatac
     8101 catgacctga gttcaacaac attgaaacga gcatatcatt attaatctct tcaccattta
     8161 aaactcgcgc cagaatttcg ctgccaaaca gttgcggtga gagaatgata tccggatgta
     8221 ccatcttaat cttattcagg tttttgctat cactgacggc gagaactgtt ttgacatcac
     8281 tgctcatatc ttttgccgag agtacaacaa acgcgttatc tgcatcgtta tcactcagcg
     8341 ccagaatggc ccggcaacga tcgattcccg ctttctttaa tacagaactg tcattactgt
     8401 caccggggat aacatcagcg ttatcgccta agcgttgctc aagttgcttg atatcatctt
     8461 caggcaagtt gctgataacc gttacgtttt gtccgcgttg attcagttgc aggatcgtat
     8521 taatggcgag aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc attgtatgat
     8581 tgtttccttt tacaagtttg ttgaatcccc cgcggataag cggaccaaaa attgaggtca
     8641 tagatgtggc aaaaacggta atgccggaaa taatgaccga aatagtgaac aatcgtgctg
     8701 attcagaaac agggacaata tcgccatagc cgacggttga catggtttct atcgaaaaat
     8761 agaacgcggt catcaaactt tctattcgcg gattaaaacc ttcgcttaaa taaagcgcac
     8821 cgtaggttga gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt gtcccggctg
     8881 cggcgctact gtgggagaag tctttgcgca gtatcagtaa aaacaccagc gtaaaaatgc
     8941 aaaatccaat actaaatttc agccagggat aaaaatgcag ggtgtaaatt agcgctatca
     9001 acaacaaaat gatactgatt gcccatgcga gctttgcccg gaacagtaag ccaatggagt
     9061 tcaggacaag aaagacgccg agcataaata gtggggcgtt tgccagcaag gagagatcca
     9121 actctgaaaa tgctttgata tggaaaatat ca
//
LOCUS       DBFIFM010000177         9088 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.177, whole genome shotgun sequence.
ACCESSION   DBFIFM010000177 DBFIFM010000000
VERSION     DBFIFM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.177"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..231
                     /locus_tag="N7C74_003743"
     CDS             <1..231
                     /locus_tag="N7C74_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HGU2433536.1"
                     /translation="LKRYAFALLAKAHSLDESMISNQTFNTYKTVLLDFNNLSNEEAN
                     QLRISSLYRDMVRYAQYRFSKVLSEWTPDAWL"
     gene            515..2665
                     /gene="dinG"
                     /locus_tag="N7C74_003744"
     CDS             515..2665
                     /gene="dinG"
                     /locus_tag="N7C74_003744"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HGU2433537.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDMLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            2693..3655
                     /gene="ybiB"
                     /locus_tag="N7C74_003745"
     CDS             2693..3655
                     /gene="ybiB"
                     /locus_tag="N7C74_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HGU2433538.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREEMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIQRCLAGREPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            3796..4881
                     /gene="hcxB"
                     /locus_tag="N7C74_003746"
     CDS             3796..4881
                     /gene="hcxB"
                     /locus_tag="N7C74_003746"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HGU2433539.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDDFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            4948..5007
                     /gene="ybiE"
                     /locus_tag="N7C74_003747"
     CDS             4948..5007
                     /gene="ybiE"
                     /locus_tag="N7C74_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HGU2433540.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(5110..5370)
                     /gene="ybiJ"
                     /locus_tag="N7C74_003748"
     CDS             complement(5110..5370)
                     /gene="ybiJ"
                     /locus_tag="N7C74_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HGU2433541.1"
                     /translation="MKTINTVVAAMALSALSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(5635..5901)
                     /gene="ybiI"
                     /locus_tag="N7C74_003749"
     CDS             complement(5635..5901)
                     /gene="ybiI"
                     /locus_tag="N7C74_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HGU2433542.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKESQLC"
     gene            complement(5975..6661)
                     /gene="ybiX"
                     /locus_tag="N7C74_003750"
     CDS             complement(5975..6661)
                     /gene="ybiX"
                     /locus_tag="N7C74_003750"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HGU2433543.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFIWIQSMIRDDKKRAMLFELDKNIQNIQSLKSRYGENEEILSLLNLY
                     HNLLREWSEI"
     gene            complement(6703..8985)
                     /gene="fiu"
                     /locus_tag="N7C74_003751"
     CDS             complement(6703..8985)
                     /gene="fiu"
                     /locus_tag="N7C74_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HGU2433544.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTATLNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTASIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
BASE COUNT         2121 a         2255 c         2511 g         2201 t
ORIGIN      
        1 ttaaaacgtt atgcatttgc tttattggcg aaagctcatt ctttagatga aagtatgata
       61 agcaatcaaa cttttaatac atacaaaacg gtattgcttg atttcaataa tctgtctaat
      121 gaagaggcca accagttgcg cataagttcg ttatatcgtg atatggtccg ctatgcacaa
      181 tatcgttttt ctaaagtttt atccgagtgg acgccagacg catggctgta aagatcgcag
      241 cagaataatg gcattgatgg ccctggctca ggccagggtc ggcaataccg gttcctgcga
      301 agcgatgacg gcggcaattg taaccacgaa atgcggtatc acttgctgtt ttctgccctg
      361 aagactggcg ctcactgtgc cgtcgaattt tcaatcaaat atattgcgag agggcgtgtt
      421 tgcgagccgc tttccagaaa cagaaaaacc attacccctg aaaaccgaaa aatgccacaa
      481 tattggctgt ttatacagta tttcaggttt tctcatggca ttaaccgccg cgcttaaagc
      541 gcaaattgcc gcctggtata aggcgcttca ggaacagatc cccgacttta ttccccgtgc
      601 gccgcagcgg cagatgattg cggacgtcgc caaaacgctg gccggagaag aagggcggca
      661 tctggcgatt gaagccccca ctggcgttgg gaaaacgctc tcctatttga ttcccggcat
      721 cgccattgcc cgcgaagagc aaaaaacgct ggtggtgagt accgccaacg tggcattgca
      781 ggatcagatc tacagcaaag atttgccact gctgaaaaag atcattcccg atcttaaatt
      841 cactgccgct tttgggcgtg ggcgctacgt ttgcccgcgt aatctgacgg cgcttgccag
      901 cactgagccc acgcaacagg atctgctggc gtttcttgac gacgaactga cgccaaataa
      961 tcaggaagag caaaaacgtt gtgcgaagct gaagggcgat ctcgacactt ataaatggga
     1021 tggtctgcgc gatcatacgg atatcgctat agatgacgat ctctggcgtc gtttaagtac
     1081 cgacaaagcc agctgcctca accgcaactg ttactactat cgcgaatgcc cgttttttgt
     1141 cgctcgtcgg gaaattcagg aagcggaagt ggtggtggca aaccatgcgc tggtgatggc
     1201 ggcgatggaa agcgaagccg tattgcctga cccgaaaaat ttactgctgg tgctggacga
     1261 aggccatcac ctgccagatg tggcgcggga tgcgctggag atgagtgccg aaatcaccgc
     1321 gccatggtat cggctacagc tggacttgtt cacgaaactg gtcgctacct gcatggagca
     1381 gtttcgcccg aagaccatcc cgccgttggc gatccctgaa cgtttgaatg cgcattgtga
     1441 agagctgtat gagcttatcg cctcattaaa caacattctc aatctctaca tgcctgccgg
     1501 gcaggaagca gaacaccgtt ttgcgatggg cgaactgcct gatgaagtgc tggagatctg
     1561 ccagcggctg gcaaaactca ccgagatgct gcgtggtctg gcggagttat ttcttaacga
     1621 tttaagtgag aaaaccggca gccatgacat tgtacgtctg catcggttga ttttgcagat
     1681 gaaccgcgcg ttggggatgt tcgaggcgca aagcaaactc tggcggctgg cttcgctggc
     1741 gcaatcttcc ggtgcaccgg tgaccaaatg ggcgacgcgg gaagagcgcg aagggcagct
     1801 acacctctgg tttcactgcg tgggaatacg tgtcagcgat cagctggaaa ggctgctgtg
     1861 gcgcagtatt ccgcacatta ttgtcacctc cgcaaccttg cgttcgctga acagtttttc
     1921 gcgtttgcag gagatgagcg ggctgaaaga gaaagcgggc gaccgttttg tggcgctgga
     1981 ttcccccttt aaccactgcg aacagggcaa aattgttatt ccccggatgc gcgttgagcc
     2041 ttccatcgac aacgaagaac agcatattgc tgaaatggcg gcctttttcc gtgagcaggt
     2101 ggagagcaaa aaacatctcg gtatgttggt gctgtttgcc agcgggcgtg cgatgcagcg
     2161 ctttctcgac tatgtgacgg atttacgtct gatgttgctg gtgcagggcg atcagccgcg
     2221 ttaccgttta gttgaactgc accgcaaacg cgtcgccaac ggtgagcgta gtgtgctggt
     2281 gggcttacag tcatttgccg aagggcttga tttgaaaggt gatatgctca gccaggtgca
     2341 tatccacaaa atcgcttttc cgcctatcga cagcccggtg gtgatcactg aaggggaatg
     2401 gctgaaaagc ctcaaccgct atccatttga ggtgcaaagc ctgccgagcg cctcgtttaa
     2461 cctgattcag caggttgggc gactgattcg aagtcacggt tgctggggcg aagtggtgat
     2521 ttacgataaa cgcttgctga ccaaaaatta tggcaagcga ctactggatg cattaccggt
     2581 atttccgata gagcaaccgg aagtccctga aggtatagtt aaaaagaaag aaaaaacgaa
     2641 atccccacgc cgtcggcggc gttaatgatg tgagtcaggt aaggagtcgt aaatggacta
     2701 tcgcaaaatc attaaagaga tcgggcgcgg gaaaaaccac gcgcgcgatt tagaccggga
     2761 tactgcgcgc ggtctgtatg cccatatgct caatggtgaa gtccctgacc ttgagttggg
     2821 cggtgtattg attgcgctgc gtatcaaagg ggaaggggaa gcagagatgc tcggctttta
     2881 cgaagccatg caaaatcaca ccatcaagct gacgccgcct gcaggcaagc cgatgccgat
     2941 tgtcatcccc agttacaacg gcgcgcgtaa acaggccaac ctgacgccgt tgttggcgat
     3001 tctcctgcac aaactcggtt ttcctgtggt ggttcacggg gttagcgaag atccaactcg
     3061 cgtgctgacc gaaaccattt tcgaactgat gggtattacg ccaacgttgc acggcgggca
     3121 ggcgcaggcg aagctcgacg aacatcaacc ggtgtttatg cctgtcggcg cgttttgccc
     3181 gccgctggaa aaacaactgg cgatgcgctg gcggatgggc gtgcgtaaca gtgcacatac
     3241 cctggcgaaa ctggcgacgc catttgccga aggtgaggca ctgcgtcttt ccagcgtttc
     3301 gcatccggaa tacatcgggc gagtcgcgaa gttctttagc gatatcggcg ggcgggcgtt
     3361 actaatgcat ggtactgaag gcgaagtgta tgctaatccg cagcgctgcc cacagatcaa
     3421 tctcattgat cgtgaagaga tgcgggtgct gtatgaaaaa caggaccctg ctggaagcga
     3481 gttactgcca caagcaaaag atccggaaac cacggcgcag tggattcagc gttgccttgc
     3541 tggccgcgaa ccgattcccg aatcgctgaa aatccagatg gcttgctgcc tggtggctac
     3601 gggtgaagcg gcaactatca gcgatggcct ggcgcgcgtt aatcaggcat tttaattctt
     3661 ttctcagccg gatgagccat gtcatccggc ttttacccca ccgcaatatg aaattcctgc
     3721 atctttattg accttcccgc gcccggcgtg cagcataaaa aaacaacaaa cacataacat
     3781 aaacaggagt taaccatgga aagtggtcat cgctttgatg ctcagacgct gcacagcttt
     3841 attcaggctg tatttcgtca gatgggtagc gaggaacaag aagcgaaatt agttgccgat
     3901 catttaatcg cggcaaacct ggcagggcat gattcacatg gtattggcat gatcccaagc
     3961 tatgtacgct cctggagtca ggggcacctg caaattaacc atcatgccaa aatcgttaaa
     4021 gaggcggggg cggcggtcac gctcgatggc gatcgcgcat ttggtcaggt cgcggcacat
     4081 gaagcgatgg cgctggggat tgagaaagcg catcagcacg gtattgctgc cgtggcgcta
     4141 cataactcgc atcatatcgg ccgtatcggt tactgggcgg agcagtgtgc agcggcgggg
     4201 tttgtctcta tccactttgt tagcgtggtc ggtattccaa tggtcgcacc gttccacggt
     4261 cgcgacagcc gctttggcac gaatccgttc tgtgtggttt tcccacgtaa agatgatttt
     4321 ccgctgttgc ttgattacgc caccagcgcc attgcatttg gcaaaacccg cgtcgcctgg
     4381 cataaaggcg tccccgtgcc gccaggttgc ctgattgacg ttaacggcgt gccgacgacc
     4441 aatccggcgg taatgcagga gtcgccgttg ggttcgctgt tgacctttgc cgaacataaa
     4501 ggctacgccc tggcggcaat gtgtgaaatt cttggcgggg cgctttctgg cggtaaaacg
     4561 acgcatcagg aaacgttaca aaccagtccc gatgccattc ttaactgcat gaccactatc
     4621 atcatcaacc cggaactgtt cggcgcgccg gattgtagcg cgcagaccga agcctttgcc
     4681 gagtgggtga aagcctcgcc gcatgatgac gataagccga ttttgctccc gggcgagtgg
     4741 gaagtgaaca cgcgtcgcga acggcaggag caggggattc cattggatgc gggaagctgg
     4801 caggccattt gtgatgcggc gcggcagatt ggtatgccgg aagagacgtt gcaggctttc
     4861 tgtcagcagt tagccagcta aaaaaaagcc cgtccagtgg cggacgggca aacaagggta
     4921 acataggatc aatgagggtt agagcatatg cgtctgtcgg caaacagaca gggaaatact
     4981 tgtgcaggac gtagcgtaaa cgcctgatcc gtccaacggt tcggattttg taggcctgat
     5041 aagacgcggt agcgtcgcat caggcatctg ttgccggatg cggcgtaaac gccttatccg
     5101 tcctacgaat tacttataaa ttaccgcagt accgcttaat ttattgttgt tggtggcgga
     5161 agtgatgcta tatccactgg caccggctgc cgccgctttc tcagccagtt tcgcttccag
     5221 ggcatcgagg gtggatgcgc catcggcaga aaccacgccg attttattca tgttctgtgc
     5281 ctgggatgcc gttaccggtt ccgcggcaaa cacgccaaat gacagagctg aaagagccat
     5341 agcagcaaca acagtattga tagttttcat aattaatctc tcgcaggtga tttttgtata
     5401 agtgacgttg tttcgtcgat gtgatgagta tcacgttttt ttacgagaga taaaatcgaa
     5461 gagaattgac cgccttgttc aaataaattg attgatatct aacatactga taaaccattt
     5521 atttgttgta cttatggact tgttttatgc agcgtggcag tcacgacagc gttaaaggca
     5581 cggaatgcca cgcaaaatac aaattatact cgctatacat ctcgccagtt gtagttaaca
     5641 taactggctt tctttcgaac ctctgcgatt atatcctgta taagctggtt tttgtaaatc
     5701 tttctcctgc tgacaatgaa tacataagcg cacgccagga atggcttccc gacgggcttg
     5761 cgggatgggg gcaccgcact cttcacattc atacaggctt tcgccacgcg gaatttcacc
     5821 ccgagcgcgg gcaatcgcat cttcaattgt actgttgatc tgttcgttga cggcgtcatc
     5881 gttagcccaa ccggatgcca tatcgtcctc cccatatcaa tgcttgtata gttaagtgta
     5941 gctaatatag ggacgaacct gggcagttca agcgtcagat ctccgaccat tcccgcagca
     6001 gattatgata aagattgagc agcgacagga tctcttcatt ttcaccatag cggcttttca
     6061 gcgactgaat attctgaata tttttgtcca gttcaaacag catggcgcgc tttttatcat
     6121 cgcggatcat cgactggatc catataaatg atgccactcg tacgccgcgg gttacgggtg
     6181 tcacgcaatg caggctgctg gagggataca acaccagatc acctgccggg agttttaccc
     6241 gatgttgtcc gaaggtgtca ttaacgacca gttcgccgcc gtcgtagctt tgtggatcgc
     6301 ttaaaaacag cgtggcagaa aggtcagtgc gcatccagcc gttttgtgga tggctgcgta
     6361 ctgcgccatc cacatgaaaa ccataggttt cattgttctg atagcgatta aacagcggcg
     6421 tggaaagggt acgcggcaag gccgcggcaa agaacaaagc atgttggtta accgcgttca
     6481 gcacctcatt ttgcagggcg gcgtataacg tgctgcgggt gtctacctgt tgattgttct
     6541 taacttgcgc accttgtgcg ccggtggtga cgcgtccatc cacccattcg gcttgttcca
     6601 gttgttcgcg aaaacgggcg acgtcctgtg gcgataacac gccgggaatg tggtacatca
     6661 tacaagttct ccaaaaagtg gggcctgcgc cccacatctg aatcagaaat gcatattggc
     6721 tgtgagcaag aaggttcttg gctcgcccgg gtgataacgg tagccgctct tattgattga
     6781 ggcgacgtaa tcggtatcaa acaggttgta gacgtttagc tggaagtcga gattgcgatt
     6841 aaccctatac cccagtttgg catcggcgac ccagtaacct tcggtaaacg ctggcgttcc
     6901 caccgcgccg tctgaacctt tatgcatact gccgatatag cgtgcgcccg cgccaacaga
     6961 gatatcatcg gttgcctgat attggctcca taaggtgaag gcgtgctccg gggtatacgg
     7021 cagcgatgag gaaccatcct gggcaacatc tttgccgttt ttgatggttg ctttttgctg
     7081 ggtatagccg ccaatcacct gccacgcggg agtgatattc cctgccacgg atatctcata
     7141 gccttcgacg cgtttcttac cgtattgcga gtaagtcccg tcatcatttt gctcaacttc
     7201 attttcgata tcagtacgga acagcgcggc ggtgagcaac aggcgtttat ccagaacctg
     7261 ccatttggtg ccaatctcgc tggtgttggc tttttgcggt ttaaaatcgg tgcggttggc
     7321 actgttaccg ctgccagact gcgcaagggc gaagttgttg ccgcccggag gctgctggga
     7381 aacggcatag ttaatataga cattgccgtt ttccgtcagg tgatacagcg ccccggcttt
     7441 ccagttcacc agattgcccg acttggcggt gtcgacggtg gtgaccggag aaccttttgc
     7501 cacaccagct gggcaggtga tggcaccgcg tccgctgccg ccgcaggcgg tggcactgtc
     7561 atattcagta tgataattat ccagacggat cccgccgttc agctcaaaat cacgggtgat
     7621 ttgcagcgta tcgaatgcgt aaattgcgaa ggtatccgtc tgaccattgg cgtttgcgcc
     7681 gttgcgcgtc aggccgccgg gatgaatgct gctgtcagga tgataaatat ttaccgcagg
     7741 taacgtcacc ggattaacgc cgtagttagt ctgcgtttca cgggtaaatt ccacgccggt
     7801 actgacatca tgaccgatag aagcggtata gaacgtcgag gtcaggttgg tctggttggt
     7861 gagaatttta ttactcacat ctttggtatt cgccgtgcgt gaccacgtcc agctattcac
     7921 atcgctggtg ggctgggtaa tattcgacgc cccacccata atcgccgtca tcaggtaatc
     7981 ctgcttcacg cgcgaccaac gggtagtgtt gcgaatggtg gtgttatcgt tgatatcgtg
     8041 ctcaaaacgc atggtggcgg tgtcggtggt cgaatcgtcg taatcggaat ccgtgccgta
     8101 aaagttatgc gtatcaactt ttccggaatg attcagggtc gccgttcctg cagatggggc
     8161 agaatagccc ggcaaaccga tggttggaat gccgccgtct ggcgtgttgt gctgggtgac
     8221 atgcagataa ttaagataca aacgattcgc tgtaccaagg ccaaaagcga tagaaggggc
     8281 gacgccgtaa cgctcatttt tgactttgtc gcgtccggca tcgtgcgttt tttcgcccat
     8341 cacattcagg cgcaccgcag tggtatcacc aatgacctga ttgacgtcca gcgtgccgcg
     8401 gcggaaccag gcgctgccaa tactggcgga ggcgtcaatg ccggaatcat tgcgcggctg
     8461 cttgctgatc atattgatcg agcccgtcgg cgcgctgcgc ccgtagtcgg tgccggaagg
     8521 ccctttaatc acttcgacct gctcagtatt gaaggtgtcg cgcgagacgc tgccgatatc
     8581 gcgaatgcca tcaatataaa tactgttaga ggtatcagca ccgcgcatat aaatggcgtc
     8641 gccagtggtg gagttaccgt tctcacccgc aaaaaacgca cccacgccgg ggacgttttt
     8701 gagcgcatcg gtaaggttgg ttgcgccctg atctttaatc acttgttcag aaatcaccgt
     8761 catcgtgcgg gtagtatccg ctaccggacg cgagaatttc ggatcggcag attgttgtgg
     8821 cgcataaagc gaaggcgttg atgcttcgac aaccagcgtg tcatccgcgt tagtttgccc
     8881 ttcggcggcg agtgcctgag ccacaggcgt gatgccaata caaagaccgg caaagaacgt
     8941 gagcgaatga aattgtctgg cagggaaatt gcgattgttt tccattttgc aggtgacttt
     9001 ttcttatatg ttgaaatttt cactgccacg agaaagcctc tctgcggatg cgatgccact
     9061 gttaatctcg tgatagtgtt cgtgcaag
//
LOCUS       DBFIFM010000178         8692 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.178, whole genome shotgun sequence.
ACCESSION   DBFIFM010000178 DBFIFM010000000
VERSION     DBFIFM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.178"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            205..915
                     /locus_tag="N7C74_003752"
     CDS             205..915
                     /locus_tag="N7C74_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HGU2433545.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(965..1939)
                     /gene="ghrB"
                     /locus_tag="N7C74_003753"
     CDS             complement(965..1939)
                     /gene="ghrB"
                     /locus_tag="N7C74_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HGU2433546.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(2043..2702)
                     /locus_tag="N7C74_003754"
     CDS             complement(2043..2702)
                     /locus_tag="N7C74_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HGU2433547.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            2855..5188
                     /gene="bisC"
                     /locus_tag="N7C74_003755"
     CDS             2855..5188
                     /gene="bisC"
                     /locus_tag="N7C74_003755"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HGU2433548.1"
                     /translation="MTNSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVNAEKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFTEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLVP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(5157..5597)
                     /locus_tag="N7C74_003756"
     CDS             complement(5157..5597)
                     /locus_tag="N7C74_003756"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGU2433549.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKVNYWRECIPLVRDAY
                     LANAKNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(5594..6157)
                     /gene="tag"
                     /locus_tag="N7C74_003757"
     CDS             complement(5594..6157)
                     /gene="tag"
                     /locus_tag="N7C74_003757"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HGU2433550.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDRKKLFEMICLEGQQTGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            6315..7013
                     /locus_tag="N7C74_003758"
     CDS             6315..7013
                     /locus_tag="N7C74_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HGU2433551.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            7242..8444
                     /locus_tag="N7C74_003759"
     CDS             7242..8444
                     /locus_tag="N7C74_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2433552.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTIASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVKFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         2033 a         2128 c         2278 g         2253 t
ORIGIN      
        1 cgtaaatctc tggctacaaa aaaggcaacg gcaatatcat tttttaacaa tgaaacaaca
       61 aattaacctt gtttcatttt ctccatgatt ttaccaggaa gattacgtag aaagggtatc
      121 ctgcaagcct gtcctggact atccttgtca cgtcagacac gcgtgtgtcg ttgtgcgctt
      181 tttttgggtg aaaggagtaa gaaaatggcg acaggaaagt cctgctctcg ctggtttgcg
      241 cctcttgcgg cgttattaat ggtagttagc ctgagtgggt gttttgataa agaaggcgat
      301 cagcgtaaag cgtttatcga cttcctgcag aatacagtga tgcgtagcgg tgaacgtcta
      361 ccaaccctga ctgccgatca gaaaaaacag tttggtcctt ttgtctctga ttacgcgatt
      421 ctgtatggtt attctcagca ggtgaatcag gcgatggatt ccggtctgcg cccggttgta
      481 gacagcgtta acgcaattcg cgtaccgcag gattacgtta cgcaaagcgg tccgctgcgt
      541 gaaatgaatg gttcgctggg tgtgctggcg caacaactgc aaaatgcgaa gttgcaggcc
      601 gatgctgcgc actctgcgct aaaacagagt gatgacctga aaccggtctt tgatcaggca
      661 ttcaccaaag tggtaacgac gccagctgat gcattgcaac cgttgattcc ggcggcacaa
      721 actttcacgc aacaactggt gatggttgga gactacattg cccaacaagg tactcaggtg
      781 agctttgtag caaatggcat ccagttcccg acttcacagc aggcaagtga atataacaaa
      841 ctgattgcgc cattaccggc acaacatcag gcgtttaatc aggcctggac tacagcagtt
      901 actgcaaccc aataaagagt aaaagcccga gcgattatcg ggctttactc cacgcagtcg
      961 cggcttagtc cgcgacgtgc ggattcacac agttcttctc aacctttcct tgtaacgcat
     1021 caatcaaatt atccacggca caggcggcca tgccataacg cgtctcatgg gtggcagatc
     1081 caatatgcgg tactgcgacg acgttggcca ttgagagcaa cggcgaatct acggaaagtg
     1141 gctcttgttc gaagacatcc agcccggcgg cgtgaatttc ccctttctgc aatgctgcga
     1201 tcagtgcatt ttcgtcaacc accgggccac gtccggcatt aatgaaaatg gcggaggatt
     1261 tcattttggc gaattgttct gcgccaaaca gatgatgcgt ctcatcagtt aacggcagga
     1321 tcaggcaaac gaaatctgac tcttgtaaca gtgtatccaa atcgcagtag cgggcgttga
     1381 agcgttcttc tgcttcttta tggtggcggc gcgcgttata gaggatgggc atgttgaagc
     1441 caaagtgcgc acgttgtgcc agcgccatac cgatccgtcc catcccgaca atgcccagtg
     1501 ttttatggtg aacgtcagtg ccgtaccagt ccgggcctat gctcgcggtc cattcgcctg
     1561 cttttacccg ttctgctacc tccacaaccc gacgagcggt agacaacacc agcgccatca
     1621 gcgtatcggc gacggtttct gttaagacgg ttggcgtgtg catcagcaga atttttcggg
     1681 cggtaagcgc atcgacatca aaattgtcat agccgacgga gatcgttgat gtggcacgca
     1741 gtttcggcat tttttccagc aatgcggcat caacattctc gtttgaaccc agtaaacctt
     1801 cagcttcggc aaaaattgct gcattttgtt cgacggtttg tgggctgagg tttgccacct
     1861 ggtgaacggt gaaatgctct tgcaggcgtt gcagtaaatc atcaggtaag gctttgtaga
     1921 ggataacgga cggcttcatg cttctctcca ttcactgata aggcgatcac cttagcctga
     1981 aatatgatga aggaaaactg aagaatgcca ggtgatttct gcatcacctg gcatgaaagg
     2041 gattacagcg ggcttaaggt aatttctaca cggcggtttt gcgccttacc ttctgcggtg
     2101 ctgttgctgg cgattgggtt agccgggcca aggccctgag tacggatgcg gctggcgtcc
     2161 acgccctggg tgatcaacgc gctggcaacg gaatccgcac gctgctggga gagacgcatg
     2221 ttcagatcgt gaccacccgt gctgtcagta taaccaatca cgttaaccgc cgttttcgga
     2281 tactctttca gcaccattgc cacgccggtc agggtgttag cgcccgccgg tttcagggtt
     2341 gcgctgctgc tgtcgaaggt cacattgttc ggcatattga ggataatgtt atccccgctg
     2401 cgggttacgc taacaccagt gccgcgcatt ttgtcacgca gcttcgcttc ctgcacatcc
     2461 atgtaataac ccacgccgcc gcccagagct gcgcctgctg ctgcgccaat cagcgcgcct
     2521 ttaccgcgat ctttcttcga agaagagagc gcaccaatac ccgcgcccac gagagagccc
     2581 agacctgcgc cgatagcaga tttacctgct tcgcgttcgc cggtgtaagg gttagttgtg
     2641 cagccagata ccgccagagc gccactcact acggcggcaa taagataaac acgtttcttc
     2701 attgttaatc cttaataacc tttttattct ttgccacggg ttccgtggcg ggagattatg
     2761 ccgcgtgaac atgaagatta ttcctgggaa tactcggaaa tttgtaagta atatttaact
     2821 gctcaataca tctaaccttt caggagtctt cggtttgacc aactcatcct cacgatattc
     2881 cgttctgact gccgcccatt gggggcccat gctggttgaa accgacggcg aaaccgtgtt
     2941 tagctcgcgt ggcgcgttag ccacaggaat ggaaaactcc ttgcagagcg cggttcgtga
     3001 ccaggttcac agcaatacgc gggtacgatt tccaatggtg cgcaaaggct ttctcgcgtc
     3061 accggaaaac ccgcaaggca ttcgtgggca ggatgaattt gttcgcgtga gttgggatga
     3121 ggcgctggat cttattcacc aacaacataa acgcattcgt gaggcttatg gtccggcatc
     3181 gatttttgct ggttcctacg gctggcgttc aaacggcgtg ctgcataagg cctcgacatt
     3241 attacaacgc tatatggcgc tggcaggcgg ttataccggg catctggggg attattcgac
     3301 cggcgcggca caggcgatca tgccgtatgt cgtgggtggt agtgaagttt atcaacagca
     3361 gaccagttgg ccgctggtgc tggaacatag cgatgtcgtg gtgctgtgga gtgctaaccc
     3421 actcaatacg ctgaaaattg cgtggaatgc atccgatgag caggggcttt cttacttttc
     3481 tgcactgcgt gacagcggga aaaagctgat ctgcattgat ccaatgcgat cggaaaccgt
     3541 cgatttcttt ggcgataaaa tggagtgggt ggcaccgcac atgggcactg atgttgcgct
     3601 gatgctgggg atcgcccata cgctggtgga aaatggttgg cacgacgaag cgtttctggc
     3661 gcgttgcacc accggttatg ccgtcttcgc ctcttatttg ctgggcgaga gtgacggaat
     3721 agcgaaaacc gccgaatggg cagcagagat ttgtggtgtt aacgcagaga aaatccgcga
     3781 gctggcggct attttccacc aaaataccac catgctgatg gctggctggg gaatgcagcg
     3841 ccaacagttt ggtgagcaaa aacactggat gatcgtcacg ctggcagcaa tgttggggca
     3901 aatcggcaca cccggcggcg gttttggtct ttcttaccat tttgccaatg gtggtaaccc
     3961 cacgcggcgc gctgcggtac tctcttccat gcagggcagc ttgccgggtg gctgcgatgc
     4021 ggtagataaa atccctgtcg cccgcattgt tgaagcactg gaaaaccccg gtggcgcata
     4081 tcaacacaac ggtatggacc gacatttccc ggatattcgt tttatctggt gggcgggcgg
     4141 tgccaacttt actcatcatc aggataccaa tcgcctgatc cgtgcctggc aaaaaccgga
     4201 gctggtggtg atctctgaat gcttctggac ggcggcggca aaacacgcgg atatcgttct
     4261 gcctgcgact acctcttttg agcgtaatga tctcaccatg accggtgatt acagtaatca
     4321 gcatctggtg ccgatgaagc aagtggtgcc gccacgctat gaagcgcgta atgattttga
     4381 tgtttttgcc gagttaagtg aacgctggga gaagggcggt tatgcacgtt ttacggaagg
     4441 aaaaagtgag ctgcaatggc tggaaacgtt ttataacgtt gcccggcagc gcggggcaag
     4501 ccagcaggtt gaattgccgc catttactga gttctggcaa gccaaccagt taattgagat
     4561 gccggaaaac ccggacagcg agcggtttat tcgcttcgct gatttttgcc gcgatccgca
     4621 ggcgcatcca ttaaaaaccg ccagcggtaa gattgaaatc ttctcgcagc gtattgccga
     4681 ttacgcttat ccggattgcc cggggcatcc aatgtggctg gtgccggacg aatggcaggg
     4741 caatgccgaa ccggaacagt tacaggtact ttctgctcat ccggcgcatc gtctgcacag
     4801 ccagctgaat tacagttctc tgcgcgaatt gtacgcggtg gcaaaccgtg agcctgtcac
     4861 cattcatcct gacgatgccc aggcgcgcgg catacaagat ggcgatactg ttcggctgtg
     4921 gaacgcacgc gggcaaattc ttgccggagc ggtcattagc gagggaatta aacctggcgt
     4981 gatttgcatt cacgaagggg catggccgga tctggattta accgctgacg gtatttgtaa
     5041 aaacggcgca gtgaacgtgc tgaccaaaga tctccccagc tcgcggctgg ggaatggctg
     5101 tgcgggtaat acggcgctgg catggctaga aaaatacaac ggtccggaac tgacacttac
     5161 agcgtttgaa ccaccggcca gctcataatc caggtgggta gttgggtttc atcctgccat
     5221 gcgcaatcga caatgtgaaa accctgcgcc tggtaaaaat ctatcgccgg ttgatttttt
     5281 tgataaacct ccagcatcag atggggatag cgctgctgca catactgcat cagcgcctta
     5341 ccaataccgc gtctgacggc cttcggtgcg acaaacattg ctgccagaaa tcggccttcc
     5401 ataatgctga caaaaccgag aagcttaccg tcttcttccc agacccagtt tttcgcattg
     5461 gcaagatacg catcccgcac cagcggaatg cactcgcgcc agtaattcac ttttataaag
     5521 ggatgccccc aggttgtact ttccagccac agttcgagga tcgcggggag ttctgaacgt
     5581 tgcgcttccc gaatcatggt ttatttcccg gatagcaaca gcagccaacc acatgatcat
     5641 tcaccagccc acatgcctgc ataaaggagt aacagattgt ggtaccgaca aacttaaaac
     5701 cacgtttttt cagtgcctta gatagggcgt cggaggcggg cgtagatgtg gggatttcgc
     5761 tcaacgttgt ggcttgtgtc acctgtggct gatgatttac aaacgaccag acaaagtcgg
     5821 caaacggttc gccgttctgt tccatttgca ggtacgcccg cgcattacca ataattgcct
     5881 gaattttccc gcgatggcgg ataatcccgg cgtcctgtac cagtctttcg acatcctctt
     5941 cctgcattgc ggcaaccttc accggatcga actgatgaaa gcaggcgcga tagttttcgc
     6001 gttttttgag gacggtgatc cacgataacc cagtctgctg cccttccagg cagatcattt
     6061 cgaacagttt tttcctgtca gtttcaggca cgccccactc attatcatgg taggcaatat
     6121 aaagcgggtc ctgactcacc cagccgcaac gttccatact ttccctcgct atgtacccga
     6181 tcgtaaaaat ttcagccgct tttcttgacg catcggtgaa aaagtcaata tttaatacag
     6241 ggctactatg cccgatatcc ttcatacaat gacaataata ttgccgaatt cggctcttcc
     6301 ctggagtcgt gttaatgatc ataaaaaaaa gcggtggtcg ctggcagcta agcctgctgg
     6361 cgagtgtggt aatcagtgcc ttttttctca acacagctta cgcctggcaa caagaatata
     6421 tcgttgatac gcaacccggg cattccacag agcgttacac ctgggatagt gatcatcaac
     6481 ctgattacaa cgatattttg tcgcaacgta ttcaaagtag ccaaagggcg ctgggactgg
     6541 aagtcaatct ggcggaagaa acccctgtgg atgtgaccag cagtatgagt atgggctgga
     6601 attttccttt atatgaacag gttacaactg gcccggtcgc ggcattacat tacgatggca
     6661 caaccacctc gatgtataac gagtttggcg acagtactac cacgctgacc gatccgttat
     6721 ggcatgccag cgtgagtacc ttaggctggc gtgttgactc ccggcttggc gatctccgac
     6781 cctgggcgca aatcagctat aaccagcaat ttggcgagaa tatctggaag gcgcaatcag
     6841 gcctgagccg gatgacggcg acaaaccaga acggcaactg gctggatgtc accgtaggcg
     6901 ctgatatgtt gctcaatcaa aatattgccg cctatgccgc gctatctcag gcagaaaata
     6961 ccactaacaa tagcgattat ctgtatacga tgggggttag tgccagattt taacgtaaca
     7021 atcacaattg aaaccattga ataacaatag ttgtggcgat agtgggtgct aacttaccaa
     7081 ataataaatt tggtgaataa ttgtcgcgtc attcattcct gaactaaggc atttcattcc
     7141 gttctgatgg catttcatgc cgtttttccc caggcataaa gtgcacttcg ttatggttgt
     7201 cggcagagat ttttcctttt tattactgca ggaatactgc catgacacct tcaaattatc
     7261 agcgtacccg ctggttgaca ctcatcggta ctatcattac ccagtttgcg ctggggtcgg
     7321 tttatacctg gagcctgttt aacggcgcgc tttccgccaa gctggatgcg ccggtaagcc
     7381 aggtcgcttt ctctttcggc ttgttaagtc tggggctggc aatatcgtct tctgttgcgg
     7441 gcaaattgca ggaacgtttt ggtgttaaac gcgtcaccat cgcttccggc attttgctgg
     7501 gattagggtt cttcctgaca gcgcattctg acaacctgat gatgctgtgg ttaagcgccg
     7561 gtgtgctggt cggtctggcg gatggcgcgg gttacctgct gacgctctct aactgcgtga
     7621 aatggttccc ggagcgtaaa ggtctgatct ccgcgttcgc tatcggttct tatggtctgg
     7681 gtagcctggg tttcaaattt atcgacacgc agctgctgga aacggtcggt ctggaaaaaa
     7741 cctttgtgat ttggggagcg attgcgctgg tgatgattgt tttcggcgca acgttaatga
     7801 aagacgcacc aaaacaggaa gtgaaaacca gcaatggtgt ggtggagaaa gattacacgc
     7861 tggcagagtc gatgcgtaaa ccgcagtact ggatgttagc ggtaatgttc ctgactgcct
     7921 gcatgagcgg cctgtacgtg attggggtag cgaaagatat cgcccaaagt ctggcacacc
     7981 ttgatgtggt ttctgcagcc aatgcagtca ctgttatttc catcgccaac ctttcaggtc
     8041 gtctggtgct gggcattctg tctgacaaaa tcgcccgtat ccgtgttatc accattggtc
     8101 aggtaatttc gctggtgggt atggcggccc tgctgtttgc accattgaat gcagtgacgt
     8161 tctttgcagc gattgcctgc gtggcattta actttggcgg cactattacc gtctttccgt
     8221 cactggtcag tgagttcttc ggcctcaata acctggcgaa aaactacggt gtgatttacc
     8281 tcggtttcgg tatcggtagc atttgtggtt cgattatcgc ctcactgttt ggcggcttct
     8341 atgtgaagtt ctacgtcatc tttgctctgc tgattctgtc attggcgctt tctacgacga
     8401 ttcgtcagcc agagcagaaa gtattgcgtg aggcgcatat ttagccagaa actacaggtt
     8461 ttgaacgaaa gggggaggaa atcctccttt tttattgcat tttaaagaat aaaaatgaaa
     8521 atattttatc ttattatttg cgtggtgaat gtttttgttt tgtttaattg gtattttata
     8581 tttatttatt cctaaaaata aacgatagct ttttatgtga aaatgtaatg caatgatttt
     8641 tttaaggttt gaaattggat gcgctattaa tatattattc gtataatttt at
//
LOCUS       DBFIFM010000179         8637 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.179, whole genome shotgun sequence.
ACCESSION   DBFIFM010000179 DBFIFM010000000
VERSION     DBFIFM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.179"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..1354)
                     /locus_tag="N7C74_003760"
     CDS             complement(<1..1354)
                     /locus_tag="N7C74_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433553.1"
                     /translation="MNRINILVICMVLFFMTGNACATEWISSEDLITSDFHLMTADER
                     NVVKAATDDSMEAAYMLKDNIRWYYHNGDLSLPANFSNKNKLVVNGNLTISGDYDDYL
                     SGNGHLIVLGNVIVDNFINHDFAYVKGQMTAKGLVYADYNDHNFEVMKGISARGIIVS
                     DKATQFEVIKAEFYINEDGSGEGYNWDENIQKAYSLVTADLYDHTEIETDNISNAYPD
                     YDSVADNIVQGLPLFRDKAAPEINEKLKWIETGKLDNFPANKIKHQDPLVARFLTHTE
                     SLSPAVMLQLLQHPDDQTRESMAQSWPAQQMHWLTDELIKDEAVARGLVKNSNISADV
                     NKKLMSVPVESVQLEQARQDNLSPDIVASLSHSPFLSVRKTLLSHYDYAWLVPTAVAD
                     ELINNEDPELRERITGADLTAQQAVMLSKDKSLKVREALARTLTELKITQLSATLRTE
                     D"
     gene            complement(1498..1782)
                     /gene="ppnP"
                     /locus_tag="N7C74_003761"
     CDS             complement(1498..1782)
                     /gene="ppnP"
                     /locus_tag="N7C74_003761"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HGU2433554.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(1854..2531)
                     /locus_tag="N7C74_003762"
     CDS             complement(1854..2531)
                     /locus_tag="N7C74_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HGU2433555.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(2789..2980)
                     /gene="yaiA"
                     /locus_tag="N7C74_003763"
     CDS             complement(2789..2980)
                     /gene="yaiA"
                     /locus_tag="N7C74_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HGU2433556.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(3030..3554)
                     /gene="aroL"
                     /locus_tag="N7C74_003764"
     CDS             complement(3030..3554)
                     /gene="aroL"
                     /locus_tag="N7C74_003764"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HGU2433557.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(3737..4195)
                     /locus_tag="N7C74_003765"
     CDS             complement(3737..4195)
                     /locus_tag="N7C74_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HGU2433558.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            4315..5124
                     /gene="proC"
                     /locus_tag="N7C74_003766"
     CDS             4315..5124
                     /gene="proC"
                     /locus_tag="N7C74_003766"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308464.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HGU2433559.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDKFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(5141..6256)
                     /gene="adrA"
                     /locus_tag="N7C74_003767"
                     /gene_synonym="dgcC"
     CDS             complement(5141..6256)
                     /gene="adrA"
                     /locus_tag="N7C74_003767"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HGU2433560.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVNLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(6358..6678)
                     /gene="psiF"
                     /locus_tag="N7C74_003768"
     CDS             complement(6358..6678)
                     /gene="psiF"
                     /locus_tag="N7C74_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HGU2433561.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(6797..8212)
                     /gene="phoA"
                     /locus_tag="N7C74_003769"
     CDS             complement(6797..8212)
                     /gene="phoA"
                     /locus_tag="N7C74_003769"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HGU2433562.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTADQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(8313..8573)
                     /gene="iraP"
                     /locus_tag="N7C74_003770"
     CDS             complement(8313..8573)
                     /gene="iraP"
                     /locus_tag="N7C74_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HGU2433563.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         2141 a         2216 c         1999 g         2281 t
ORIGIN      
        1 tatcctctgt acgcagggtt gctgacagtt gggtaatttt taattcagta agcgtccggg
       61 ctaatgcttc acgaactttc agtgatttat ctttactcaa catcactgct tgttgtgcgg
      121 ttaaatctgc tccggtaatt ctttcacgta actccgggtc ttcgttattt atcaactcat
      181 cggccactgc agtcggaacc agccaggcgt aatcgtaatg gctgagtaat gtcttgcgta
      241 cactgagaaa ggggctatga cttaacgacg caacaatatc tggagaaaga ttatcctgtc
      301 tggcctgctc cagctgtacg ctctcgacgg gtacagacat taatttttta ttcacatcgg
      361 cggaaatatt actgttttta accagccctc tggcaacagc ctcatcttta attaattcat
      421 cggtaagcca atgcatctgt tgagcgggcc aggactgcgc catcgattca cgcgtctggt
      481 catcggggtg ctgtaacagt tgcagcataa cggcgggtga taaactctct gtatgagtca
      541 gaaaacgtgc caccagtgga tcttgatgct ttattttatt cgctgggaaa ttatcgagtt
      601 tccccgtttc aatccatttt aatttctcat taatttcagg agcggcttta tctcgaaaaa
      661 gcggtaaacc ctgaacgatg ttgtctgcga cgctatcata gtcaggatac gcattggaaa
      721 tgttatcggt ttcgatttca gtgtgatcat aaagatcggc agtcaccagt gaataggctt
      781 tttgaatatt ctcatcccag ttatatcctt caccacttcc atcttcattg atataaaatt
      841 cagctttaat gacttcaaat tgcgttgctt tatcggaaac aataatgcct cgcgcactaa
      901 ttcccttcat cacttcaaag ttatgatcat tgtaatcagc ataaactaaa cctttcgctg
      961 tcatctgtcc tttgacatac gcgaaatcat gattaataaa gttatcgaca ataacattac
     1021 ctaatacaat cagatgacca ttacctgaca gataatcatc ataatcgccg gaaatcgtta
     1081 aattaccgtt caccaccagt ttatttttat tgctgaagtt agcgggtaat gataggtcac
     1141 cattatgata ataccagcgg atattatctt ttagcatata agcagcttcc atactatcgt
     1201 cggttgccgc tttaaccacg ttgcgctcat ctgccgtcat aagatgaaag tcagaggtga
     1261 taaggtcctc gctactgatc cactctgtgg cacatgcatt gccagtcata aaaaataaga
     1321 ccatacagat gaccaatata ttaattctat tcataatctt caatgatatt tatatcaacc
     1381 ttgatgaatt atgaatatat cggaaattga aaaaatcgca gcaaaggaaa tattattatg
     1441 gggttatgag aggacatttc agaaaaatgc cctcccgttc aagcggaagg cgaggaatta
     1501 cagatagcgg cacagataag aggtgggttc ggcaacttgc agatgaaact cactgtgacc
     1561 gggaacatta aacaccgaac cggcttcata cacttgccag tcggtcgcgt caggcagtaa
     1621 cacattcagc gcgccactga ttaccgtcat ctcttccggc tcagcggtgc tgaaggtgta
     1681 ttcgccttca accataacac ccacgctggc gcgaccagtg ctgctgctgg aaaagccgat
     1741 cgatttcact ttgccggaaa agtactcatt actttgaagc ataaactggc cctgtctggt
     1801 agttgaatat ctacttcact ataggggcca gatgcgctgg ctgtcacgca aaattacacc
     1861 agtaattccg cagccagccg tgcaatcaat acgttagaga gcaagacagg aacatcgagc
     1921 tgtttttgca gtaaatcgcg atgacgctgg ttaaatccca aacaatccag catgatgaca
     1981 tctgcgcctt tcgccagtaa ttctttcccg gcatcaatga ttttttgttc tgaatcatga
     2041 atggggttac ccaatgaaaa tacaggcggt ttctgcaaaa tttgccattt ttgcgcctga
     2101 acggtcagca actcctcaac cggtacgata acccccacct gatgatcttc aacaatagag
     2161 gaaaccagcg gtggcaatat ccgcgacggc tcaagaaaga tcgtattacg cgcagtcata
     2221 ctactaatgt ttgccgtact cattaatata atgacgtcgt agccctgatt atcgagcact
     2281 tcaaccacac cttgcaggtc acgctctact ttgcgacgcg aaacatgggc cagatggttg
     2341 tcatttaata atgtgagaat tgtgtcttcg cctgcttctg gcgcgtactc cgccatcact
     2401 tcttcgcggc ttaacttccc cagcaggcta tgatgggaaa tattatcttc gtcaatgtat
     2461 tctgtcagga gcggcaaaac ttcctgcata ggtacaatgc cgatggttag aatcgccaac
     2521 gacgcactca ttgttcttcc accttacttt tatttcgtcg ttaattaccg ggcaagtgtt
     2581 aagcaccact gctgacactt acttgtgcta caaatgacaa aaagcgtagc agcagacgtg
     2641 cacggcataa tgtaaagatt ccaaatgatt ccagtaatgg attgttattg tttaatattc
     2701 taattatttg aaaaacatga attatgaaaa atgtgacgca gatcccttgt agtggcactc
     2761 agaatccctt ccgagcagtc tgatgcggtc actctttgtc agggtcctca tagcgttttt
     2821 tcgcatccat cgcttcctga gcggtcggat gttcactgat caacgagtct ggtttaggat
     2881 gatcggctct gagttggtac caggttaccg tctgtcctgg ctttcctttt tcaattgtca
     2941 ctatatatgc ttcacgagga taaggcggtt tcgttggcat cgttcttcct tatttcacgg
     3001 gatgaacgtt aagtataggc gctcgaaaat caacaattga tcgtctgcgc cagggcgctg
     3061 cgaatttcag aaatcacctg gctgggttcg tttgttgcgt cgatgataat atgcgcaact
     3121 tcgcgatata gcgcatcgcg ttcttccagc acttcctgaa cttcttcgct cagcggtttt
     3181 cccgttaagg ttggccgtaa atcttcttcc ggtgcagctt gcagtcggtt aaccaggact
     3241 gatactggcg cacacaaata aaccacgatc ccgttatttt gcatgaagtg acgattaaat
     3301 tccgtcagaa taatgccgcc gcctgtagcg ataacggtgg atggcgcagt taccgcttcc
     3361 agcgccgccg tttctctggc gcgaaatccc gcccactctt ccctttcgac gatctccgcg
     3421 accgtcatat tgagctgtga ttgcaaccac tgatcggtat cgacaaaccg acggttaagc
     3481 gaatcggcaa gggccattcc gaccgttgtt ttaccacagc cccgaggccc gatcagaaaa
     3541 agaggttgtg tcatcgtggg ttttccccaa taggtcgcaa tgcggcgaaa gccggtgtca
     3601 tgagaatagc gatcatacca tcaaactagt acaatttcga ttgtaaagaa aaaattccac
     3661 ttaaagtgaa aatctcaata catcccttac tataccaata aatattcaag aatgaagtgt
     3721 aaataataaa ttacatttag ccacgactac gttgcacttc cagccaccac ttctccagct
     3781 ccgcggcaaa ggcctggcgg tcacgttgtg aaaggctatc tggtccgccg gtctggatcc
     3841 cactggcacg taaggtatcc ataaaatcgc gcatcgtcag gcgctcacga atggtcgctg
     3901 gcgtgtaacg ttcaccgcgc ggattaagcg ccgcagcgcc tttctcgatg gcttcagcag
     3961 ccaaaggtat atctgcggtg atcaccaaat ctcccgcttc acactgccgg acaatttcgt
     4021 tatcggcaac gtcgaaacct gccgcgacgc gcagcgtacg aataaatcgc gatggcggca
     4081 cgcgtaaact ctggtttgct accagtacca gcggcatctg catacgttcc gccgcgcgat
     4141 acaaaatctc tttaattaca ttgggacacg cgtcggcatc cacccaaatt gtcataaagt
     4201 catcctttgt tgggtaatcc tctattgtgt cgcgcttttg ccttccggca tagttctgtt
     4261 tatgcttctg ccagcgatta tcaaaacaat gaatttcacg gcaggagtga ggcaatggaa
     4321 aagaaaatcg gttttattgg ctgcggcaat atgggaaaag ccattctcgg cggtctgatt
     4381 gccagcggtc aggtgcttcc agggcaaatc tgggtatata ccccctcccc ggataaagtc
     4441 gccgccctgc atgacaagtt cggcatcaac gccgcagaat cggcgcaaga agtggcgcaa
     4501 atcgccgaca tcatttttgc cgccgttaaa cctggcatca tgattaaagt gcttagcgaa
     4561 atcacctcca gcctcaataa agactctctg gtcgtttcta ttgctgcagg tgtcacgctc
     4621 gaccagcttg cccgcgcgct gggccatgac cggaaaatta tccgcgccat gccgaacact
     4681 cccgcgctgg ttaatgccgg gatgacctcc gtaacgccaa acgcgctggt aaccccagaa
     4741 gataccgctg atgtgctgaa tattttccgc tgctttggcg aagcggaagt aattgctgag
     4801 ccgatgatcc acccggtggt cggtgtgagc ggttcttcgc cagcctacgt ctttatgttt
     4861 atcgaagcga tggccgacgc cgccgtgctg ggcgggatgc cacgcgccca ggcgtataaa
     4921 tttgccgctc aggcggtaat gggttccgca aaaatggtgc tggaaacggg agaacatccg
     4981 ggggcactga aagatatggt ctgctcaccg ggaggcacca ccattgaagc ggtacgcgta
     5041 ctggaagaga aaggcttccg tgccgcagtg atcgaagcga tgacgaagtg tatggaaaaa
     5101 tcagaaaaac tcagcaaatc ctgatgactt tcgccggacg tcaggccgcc acttcggtgc
     5161 ggttacgtcc ggctttcttt gctttgtaga gcgccaaatc tgccgatttc aaccactcgc
     5221 gatagtgact catttgtggg ttcagcggcg caacccccac actaatccgt aaatttacct
     5281 gtggcgtatt cggcaaacgt aatgtattta gcccttcatg cacccgtaac atggcggtaa
     5341 tggcgctctc agctggcgta ccggacatga ttaccgcaaa ctcatcgccg ccaaaccgac
     5401 caatcacatc gctaccgcgc agggtaattt gtaactgtcg ggtaagcgcc acaatcgctt
     5461 catcgcccac atcatggccc caggtatcgt tgatgctctt gaaatggtcg atatcgataa
     5521 tcagtaacgt tgcatcgcga ttatgccgcc gacagttatc aaattcattg cgtaacatag
     5581 tttcccaatg acgtcggtta tacacgcccg tcatgccgtc gcgggtactc atgacctgca
     5641 acctgcgttt atgttccgcc agtttggttg ccgtctggta gctgacccag ccaaacagca
     5701 gaggataaat gacaataatg ggaagggaga gccaccattc cagcggcgca ctattgaacg
     5761 acacggtaat gcccgtcagc tcgagggtga caaggcagga aaccaccatc aacaccagac
     5821 ccgcgacaaa cagacggggg ccgcctgccc ccatcaaatt cagacacata atcatcaaca
     5881 tcgcggtgga aggcagcacg tttacgccca ttacgcctac ccacattccc gctaatactg
     5941 catcggtttt taagttgtaa atttcccggc taagcggatc gacggccctg ctcgctatct
     6001 gccaggctaa atgcggccag acgaacgccc agccgaccaa caccagccac caccagcccg
     6061 gcggcgggtg tgaaaccagc gttgaagcaa tcggtaagaa catgccagcc aggccaaccg
     6121 cacggggtag tctgacgcga cgggcgaagc gcagcccgga ccgctgatgt tcgttttgag
     6181 gagttaaagg aggttcctcc ccgtgcgccg ccgctttttt gaaaaagttt tcatcattca
     6241 ttatttttgg gaacattcag aaacaatttt cccaaattat agagacgggg ctaaaggcag
     6301 aagtatgaaa tttcgggtga gccattttag tagctcaccc gttagcacta tcaggtatca
     6361 ggcggctttc ttgaggcagg cgctcataaa cttattacga tcatcacctt tcagtgattg
     6421 ttgcgttgct tgattattac attcgcgcat cttttgctgc tgtggcgtca aacttttttc
     6481 accaggcgca gacttgctgt tcttcaggca atcactcatg taggtcttac gagcatcccc
     6541 tttcaacgcc tgtgccgtcg cctgctgatt acaggaggtc atacgctgtt gttgtggggt
     6601 taaggttctc tcggcagcgc cgacggtggt taaaaaaacc agaccgaaaa gcaaggtaac
     6661 cagtaatgta attttcatag caccatccct cttcatgttt taaccatgag cgtatgcgcc
     6721 cgtgatctga cattaagtct ggttgctaac agcaaaaaaa ccacccggca gcgaaaattc
     6781 actgccggga gcggttttat ttcagcccca gagcggcttt catggtgtag aagagatcgg
     6841 tctggtcggt cagtccaacg acattggcgg catgcgggcc atacgccgca atacgcaact
     6901 gactgccggt atgttcttgt gaatcctctt cggagttccc gtaactcatc accatcactg
     6961 cgccatcttt ggtatttagc gcctgagtga ggcccggagc tttggtatcc ggcgcaacaa
     7021 tctggctggc gtgggcgtga tcagcggtga ctatgaccag cgtgttacca tcctttttgg
     7081 cgaattccag cgcccgttgt acggcttcat cgagatcgac cgtctcgcca atttgcccac
     7141 aaggattcgc agcatgatcc tgtttatcga ttgacgcacc ttcaacttgc aggaaaaagc
     7201 ctttctcatt tttactcaac aattcaatgg ctttgtcggt catctgtgcc agggtcggta
     7261 cactgtcatt acgttgcgga ttaggcgtac aggtgactgc gggcttatcg atattaccgt
     7321 ggtacgttgc tttcggtcct agccagcgca ctggcatatt gccgtcagca aacaggccaa
     7381 gcaggggttt ttgctgattc gcttccgtca ccgaattcag tgaggcagca tcgctcacca
     7441 actgataacc acgcgcctgt gcctgttcac gcagcgtttt tccctgccat tcaccagcgg
     7501 ttgccgtttc agcaaaggtt tttgcgccgc cgccaagcgt aacgtcggca cgagcgttaa
     7561 gcagctgttc ggtaatcgat ccttttccgc ctttttccag agcgttaccc ggacattttt
     7621 cactggtcgc gctcggaccg tagcatttgc gcgaggtcac atgcgccacc agcgcggcgg
     7681 gcgtggcatc ctgcaactct gcggtagaaa cgttaccggt cgccagacct gcggcttttg
     7741 ccatttccag aatcgttggg tgatcttttt cgtgaatatc gacgcccagc gcgccgttat
     7801 aggttttgac accggttgac caggcggttg ctgatgcagc cgagtcggtg acgtagtccg
     7861 gttttccggt ttttttattc agcgcatagt gagtgtattg cccggtaagc ggtaaggcat
     7921 cgataccttt aaaaaagccg cccgcacctt cggcataatt acgtgcggca gtaatttccg
     7981 aatcccccat cccatcgcca atcagcaaaa taatattttt tgcaggttta tcgctaagag
     8041 aatcacgcag agcggcggtc tgatcagccg ttaagcggcg agcaccgccg ggtgcagtaa
     8101 tatcgccctg agcagcccgg ttttccagaa caggcatttc tggtgtccgg gcttttgtca
     8161 caggggtaaa cagtaacggt aagagtgcca gtgcaatagt gctttgtttc actttatttt
     8221 ctccatgtac aaatacatta aaaaataaaa acaaagcgac tataagtctc ggccgtgaca
     8281 actttatgac agctgttgaa aagattaact ttttactgac gaggatgctt caataacttc
     8341 tttacgtaat cgcgcagcag ctccgtatcg tcgtcaggaa tgctggcatc gggctttacc
     8401 tcgtacagcg ccccctctac ctgatcaatc aaccgctgtt ggtcattttg cgccatattg
     8461 cgaagcattg cagtgacgat aatctccaaa gcttctacct gcgcacacag ttctttcgac
     8521 tcttcttctt tttgggcaag cttaaataac aactcagcaa tgagattttt catgtctgta
     8581 tttccttatc caaagtatgg agaagttaac attatgtttc acaacttcat agtgtct
//
LOCUS       DBFIFM010000180         8603 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.180, whole genome shotgun sequence.
ACCESSION   DBFIFM010000180 DBFIFM010000000
VERSION     DBFIFM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.180"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(158..370)
                     /gene="rpmE"
                     /locus_tag="N7C74_003771"
     CDS             complement(158..370)
                     /gene="rpmE"
                     /locus_tag="N7C74_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HGU2433564.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            573..2771
                     /gene="priA"
                     /locus_tag="N7C74_003772"
     CDS             573..2771
                     /gene="priA"
                     /locus_tag="N7C74_003772"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HGU2433565.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGHAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2927..3952
                     /gene="cytR"
                     /locus_tag="N7C74_003773"
     CDS             2927..3952
                     /gene="cytR"
                     /locus_tag="N7C74_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HGU2433566.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4044..5003
                     /gene="ftsN"
                     /locus_tag="N7C74_003774"
     CDS             4044..5003
                     /gene="ftsN"
                     /locus_tag="N7C74_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HGU2433567.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVTRAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5096..5626
                     /gene="hslV"
                     /locus_tag="N7C74_003775"
     CDS             5096..5626
                     /gene="hslV"
                     /locus_tag="N7C74_003775"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HGU2433568.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5636..6967
                     /gene="hslU"
                     /locus_tag="N7C74_003776"
     CDS             5636..6967
                     /gene="hslU"
                     /locus_tag="N7C74_003776"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HGU2433569.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7034..7960
                     /gene="menA"
                     /locus_tag="N7C74_003777"
     CDS             7034..7960
                     /gene="menA"
                     /locus_tag="N7C74_003777"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HGU2433570.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVVGIFLSQWAA"
     gene            8053..8538
                     /gene="rraA"
                     /locus_tag="N7C74_003778"
     CDS             8053..8538
                     /gene="rraA"
                     /locus_tag="N7C74_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HGU2433571.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
BASE COUNT         2095 a         2226 c         2349 g         1933 t
ORIGIN      
        1 taaaatcctt ttgaaataaa taagaaccag aagcctagcg ccagtcaaca acaggaaatg
       61 ataatgagtt aacaccccgg aatttttcag aatttataaa aggcaaatta caggcacaaa
      121 aaaagcgccg tgcggcgctt ttttcggaaa tccggtctta tttgctgccc gggatgttga
      181 aacgcttgtt gaagcggtca acacggccac cggtagcaac atcacgctgt ttgccagtga
      241 agaacgggtg gcacttgctg cacacgtcga ggttcaggtc atgaccaacg gtggagcgga
      301 ttttcattac gttaccgcaa gagcagctag cagtaatttc ttcgtatttc gggtgaatat
      361 cttttttcat gggaaaacct cggtttaagg ccgcgtcgct cttccagccc taacgccaga
      421 caccacgcga tgttaaaagt atagcttcaa tacgatcatt tcgtacgaag gcgcgaaatc
      481 atacagaaat taaccagcat atgcaaactg atccgcactc ttctacggca atgtgtatac
      541 taacccaccg aatttcaagt caggatgatg ctatgcccgt tgcccacgtt gccttgcccg
      601 ttccgcttcc tcgtaccttt gactatctcc tgccagaagg catggcggtt aaagccgggt
      661 gtcgcgtgcg cgtgccgttt ggcaaacagc aggagcgcat cggggttgtg gtgtcagtta
      721 gcgacgtcag tgagctgccg cttaacgagc taaaagcggt agtcgaagtg ctggatagtg
      781 agccggtgtt tactcactcc gtctggcgat tgctgctctg ggcggcggat tactatcatc
      841 atccgattgg cgatgtgctg tttcatgcct tgccgatttt actgcgccag gggcggcctg
      901 cggcgaacgc gccgatgtgg tactggtttg ccactgaaca aggccatgcg gtggatctga
      961 acagcctgaa acgttcgcca aagcaacaac aggcactggc ggcattgcgg caaggcaaaa
     1021 tctggcgcga ccaggttgcc acgcttgaat ttaatgatgc cgcgttacag gcgctgcgca
     1081 aaaaaggtct gtgtgattta gcaagtgaaa caccagagtt tagcgactgg cgaacgaact
     1141 atgccgtttc tggtgaacgg ttgcgattga ataccgaaca ggccaccgcc gttggcgcaa
     1201 ttcatagcgc ggcggatacc ttctctgctt ggctgctggc gggcgttaca ggttccggta
     1261 aaacagaggt ttatttgagc gtactggaaa acgtgctcgc tcagggcaaa caggcgctgg
     1321 tgatggtgcc ggaaatcggc ctgacaccgc aaactatcgc ccgttttcgt gaacgtttta
     1381 atgcccccgt agaagttctg cattccggcc tgaacgacag cgagcgtctt tcggcgtggt
     1441 tgaaagcgaa aaatggtgag gcggcgattg tcatcggcac ccgctccgcg ctgtttacgc
     1501 cgtttaaaaa tctcggtgtg attgtcattg atgaagagca cgacagttcc tacaagcagc
     1561 aggaaggctg gcgctatcat gcccgcgacc tggcggttta tcgtgcgcac agcgagcaaa
     1621 tcccgattat tcttggctcc gcaacacccg cactggaaac gttatgcaac gtccagcaga
     1681 aaaaataccg cctgctgcgc ctgacgcgtc gggcggggaa tgcgcgtccg gcaattcaac
     1741 atgtgctgga tttaaaaggt cagaaggtgc aggcggggct ggctccggcg ttaatcaccc
     1801 gtatgcgcca gcatttacag gccgacaacc aggttatcct ctttcttaac cgccgtggct
     1861 ttgcgcctgc cttgctgtgc cacgactgcg gctggattgc cgaatgtcca cgttgcgatc
     1921 actactacac gctgcatcag gcgcaacagc atttgcgctg tcaccactgt gacagccaac
     1981 gcccggtgcc gcgccagtgc ccttcctgcg gttccacgca cctggtaccc gtggggctgg
     2041 gcaccgaaca gcttgaacag acgctcgcgc cgttattccc tggtgtgccc atttctcgta
     2101 tcgaccgcga taccaccagc cgcaaaggag cgctggaaca acaactggcg gaagtgcatc
     2161 gcggcggcgc gaggatattg attggcacgc aaatgctggc gaaaggtcac cactttccgg
     2221 atgtgacgct ggtagcgtta ctggatgtgg acggcgcgct gttttctgcc gattttcgct
     2281 cggcagagcg ttttgcccag ctatacaccc aggtagcagg ccgtgccggg cgtgcgggta
     2341 aacagggcga agtggtgctg caaacgcatc atccggaaca cccgctattg caaacgttgc
     2401 tctataaagg ctacgacgct tttgccgaac aggcactggc tgagcggcga atgatgcaac
     2461 tgccgccgtg gaccagccat gtgattgtgc gtgcggaaga tcataacaat cagcacgcgc
     2521 cattgttcct gcaacaactg cgtaatctga tcctctccag cccgctggca gacgacaaat
     2581 tgtgggttct cggtccggtt ccggcactgg cacctaaacg tggcggtcgc tggcgctggc
     2641 agatattgtt gcagcaccct tcccgcgtgc gcttgcaaca catcattaac ggtacgctgg
     2701 cactcatcaa tacaataccg gattcccgta aggtgaaatg ggtgctggat gttgatccga
     2761 ttgagggtta aaccgctcac gatgcgaggc ggatcgaaaa attcaatatt catcacactt
     2821 ttcatgaaaa ttctgtaacc gttttcacgc gctatctgct aaaaatgttg ccgatgtgaa
     2881 gtaaacatgg atgtagtacg cctgacgtgc caggcgagga gtgagtgtga aagcgaagaa
     2941 gcaggaaact gccgcgacca tgaaagacgt tgccctcaag gcaaaagtct ctacagcgac
     3001 cgtctcccga gcattaatga atcccgataa agtctcccaa gccacccgta atcgggttga
     3061 aaaagcggcc cgtgaagtgg gttatttacc gcagcctatg gggcgcaacg tcaagcgtaa
     3121 tgaatcccgc accattctgg tgattgtccc ggatatctgc gatcccttct ttagcgaaat
     3181 tattcgcggt atcgaagtta cggcggcaaa tcacggatat ctggtgctga ttggcgactg
     3241 tgcgcatcaa aatcagcagg aaaaaacctt tatcgatttg atcatcacca agcaaattga
     3301 tggcatgttg ctgctgggtt caaggctgcc gtttgatgcc agcattgagg aacagcgtaa
     3361 tctgccgccg atggtgatgg cgaacgaatt tgcaccggaa ctggagctgc ctaccgttca
     3421 tatcgacaat ctgaccgccg catttgatgc agtaaattat ttatatgagc aagggcataa
     3481 acggattggc tgtatagccg gtcccgaaga gatgccgctg tgtcactacc gcctgcaagg
     3541 ttacgttcag gcgctgcgtc gctgcggcat tatggttgat ccgcaataca tcgcccgtgg
     3601 cgacttcacc ttcgaagccg gaagcaaagc gatgcagcag ctgcttgatc ttccacaacc
     3661 gcctactgct gtcttctgcc atagcgatgt gatggcgctc ggcgcacttt ctcaggcaaa
     3721 acgccagggg ctgaaagtcc cggaagacct ttccataatc ggttttgata acatcgacct
     3781 gacgcaattt tgtgatccgc cgctgacaac catcgcgcag ccgcgttacg aaatcggtcg
     3841 ggaagctatg ctgttattgc ttgatcaaat gcaggggcaa cacgttggca gtggctctcg
     3901 tttaatggac tgcgaactta tcatccgggg atcaacacgc gcgttacctt aaagtaaacc
     3961 tttaagactg ccgtgactgg tcaaaggccc gccgcttaag taacatggcg ggctgacgaa
     4021 cgaataaata cagcgaaacg atagtggcac aacgagatta tgtacgccgc agccaaccgg
     4081 caccttcgcg gcgaaaaaag agcacctcac ggaaaaagca acgaaatctg cctgcggttt
     4141 ctcccgctat ggtcgctatt gctgccgccg ttcttgtgac ctttatcggt ggtctgtact
     4201 tcattacgca tcacaagaaa gaagagtccg agacgctgca aagccagaaa gtgaccggaa
     4261 acggactacc accaaaacca gaagaacgct ggcgctacat caaagagctg gaaagtcgcc
     4321 agccgggtgt gcgtgcgccc acagaacctt ctgccggtgg tgaagtgaaa acgccggagc
     4381 aactgacacc agaacaacgt cagcttcttg aacaaatgca ggctgatatg cgccagcagc
     4441 caacgcagct ggttgaagtt ccgtggaatg aacagacgcc ggaacagcgt cagcaaacgc
     4501 tacagcgcca acgtcaggcg cagcaattag cggaacagca acgcctggct cagcagtcgc
     4561 gtacgactga acaaagctgg cagcagcaga cgcgtacgtc gcaagccgcg ccggtacagg
     4621 cgcaaccgcg ccagtctaaa ccggcttcta ctcagcagcc gtaccaggat ctgctgcaaa
     4681 cccctgcgca cacgaccgcg cagtcgaagc cgcagcaagc tgcgccggtt actcgtgccg
     4741 ctgacgcgcc aaaaccgacg gcggagaaaa aagacgaacg ccgctggatg gtgcagtgcg
     4801 gttcgttcag aggcgcggaa caggcagaga cggtacgtgc tcagctggcg ttcgaaggct
     4861 ttgactcaaa aattaccacc aacaatggct ggaatcgtgt ggtcattggc ccggtgaaag
     4921 gcaaagagaa cgcagacagc accctcaatc ggttgaagat ggcgggtcat acaaactgca
     4981 ttcggctcgc cgccgggggt tgaaaccctc aaaatccccc ccatctataa ttgcattatg
     5041 ccccgtactt ttgtacgggg tttgtactct gtattcgtaa ccaaggggtc agctcgtgac
     5101 aactatagta agcgtacgcc gtaacggcca tgtggtcatc gctggtgatg gtcaggccac
     5161 gttgggcaat accgtaatga aaggcaacgt gaaaaaggtc cgccgtctgt acaacgacaa
     5221 agtcatcgcg ggctttgcgg gcggtactgc ggatgctttt acgctgttcg aactgtttga
     5281 acgtaaactg gaaatgcatc agggccatct ggtcaaagcc gccgttgagc tggcaaaaga
     5341 ctggcgtacc gatcgcatgt tgcgcaaact tgaagcactg ctggcagtcg cggatgaaac
     5401 tgcatcgctt atcatcaccg gtaacggtga cgtggtgcag ccagaaaacg atcttattgc
     5461 tatcggctcc ggcggccctt acgcccaggc tgcggcgcgt gcactgttag aaaatactga
     5521 acttagtgct cgtgaaattg ctgaaaaggc gttggatatt gcaggcgaca tttgcatcta
     5581 taccaaccat ttccacacca tcgaagaatt aagctacaaa gcgtaaggat ctcccatgtc
     5641 tgaaatgacc ccacgcgaaa tcgtcagcga actggataag cacatcatcg gccaggacaa
     5701 cgccaagcgt tccgtggcga ttgctctgcg taaccgctgg cgtcgcatgc agctcaacga
     5761 agagctgcgc catgaagtga ccccgaaaaa tatcctgatg atcggcccga ccggtgtcgg
     5821 taaaactgaa atcgcccgtc gtctggctaa gctggcgaat gcgccgttca tcaaagttga
     5881 agcgaccaaa ttcaccgaag tgggctacgt cggtaaggaa gtggattcta ttattcgcga
     5941 tctgaccgat gccgccgtga aaatggtacg cgtccaggct atcgagaaaa accgttatcg
     6001 cgctgaagaa ctggcagaag aacgtattct cgacgtgctg atcccacctg ctaaaaacaa
     6061 ctggggacag accgaacagc agcaggaacc gtccgctgct cgtcaggcat tccgcaaaaa
     6121 actgcgtgaa ggccagcttg atgacaaaga aatcgagatc gatcttgccg cagcaccgat
     6181 gggcgttgaa attatggctc ctccaggcat ggaagagatg accagccagc tgcagtccat
     6241 gttccagaac ctgggcggtc agaagcaaaa agcgcgtaag ctgaaaatca aagacgccat
     6301 gaagctgctg attgaagaag aagcggcgaa actggtgaac ccggaagagc tgaagcaaga
     6361 cgctatcgac gctgttgagc agcacgggat cgtgtttatc gacgaaatcg acaaaatctg
     6421 taagcgcggc gagtcttccg gtccggatgt ttctcgtgaa ggcgttcagc gagacctgct
     6481 gccgctggta gaaggttgca ccgtttccac caaacacggg atggtcaaaa ctgaccacat
     6541 tctgtttatc gcttctggcg cgttccagat tgcgaaaccg tctgacctga tcccggaact
     6601 gcaaggtcga ctgccaatcc gcgttgaact gcaggcactg accaccagcg acttcgagcg
     6661 tattctgacc gagccgaatg cctctatcac cgtgcagtac aaagcactga tggcgactga
     6721 aggcgtaaat atcgagttta ccgactccgg tattaaacgc atcgcggaag cggcatggca
     6781 ggtgaacgaa tctaccgaaa acatcggtgc tcgtcgttta cacactgttc tggagcgttt
     6841 aatggaagag atttcctacg acgccagcga tttaagcggt caaaatatca ctattgacgc
     6901 agattatgtg agcaaacatc tggatgcgtt ggtggcagat gaagatctga gccgttttat
     6961 cctataatcg cgttcaatca ttttcatcat tgtttgatgg ggctgaaagg ccccattttt
     7021 attggcgcgt attatgactg aacaacaaat tagccgaact caggcgtggc tggaaagttt
     7081 acgacctaaa accctccccc tcgcctttgc tgcaattatc gtcgggacgg cgctggcatg
     7141 gtggcaaggt cattttgatc cgctggtcgc cctgctggca ctgattaccg ccgggctatt
     7201 acagatcctt tctaacctcg ccaatgatta cggcgatgcg gtaaaaggca gcgataaacc
     7261 tgaccgtatc ggaccgctac gcggcatgca aaaaggggtc attacccagc aagagatgaa
     7321 acgggcgctc attattaccg tcgtgctcat ctgtctctcc gggctggcac tggttgcagt
     7381 ggcgtgccat acgctggccg attttgtcgg tttcctgatt cttggcgggt tgtcgatcat
     7441 tgccgctatc acctacaccg tgggcaatcg tccttatggt tatatcggtc tgggtgatat
     7501 ttccgtactg gttttctttg gctggttaag cgtcatgggg agctggtatt tacaggctca
     7561 tacattgatt ccggcactga tccttccggc gaccgcatgc ggcctgctgg caacggcagt
     7621 actgaacatt aataacctgc gtgatatcaa tagcgaccgc gaaaacggca aaaacacgct
     7681 ggtggtgcgc ttaggtgaag tgaacgcgcg tcgttatcat gcctgcctgc tgatgggctc
     7741 tctggtatgt ctggcgctgt ttaatctctt ttcgctgcat agcctgtggg gctggctgtt
     7801 cctgctggcg gcaccgttgc tggtgaagca agcccgttat gtgatgcggg aaatggaccc
     7861 ggtggcgatg cgaccgatgc tggaacgcac tgtcaaggga gcgttactga ctaacctgct
     7921 gtttgttgta gggatattcc taagccagtg ggcagcataa ctgacaaata tcaattaaca
     7981 attgatgatt ttgccaacag accacatagc gcgatatact gaaaattctc gcagcaactg
     8041 aatgttaagc ctatgaaata cgatacttcc gagctttgtg acatctatca agaagatgtt
     8101 aacgtcgtgg aaccgctgtt ctccaacttt ggcggacggg cgtcgtttgg cggacaaata
     8161 atcacggtaa aatgtttcga ggacaacggg ttgctgtacg atctgctcga acagaatggc
     8221 cgtggtcgtg ttcttgtcgt cgatggcggt ggttctgttc gtcgcgcact ggtcgatgct
     8281 gaactggcgc gtctggcagt acaaaatgaa tgggaaggtc tggtcattta cggcgcggtg
     8341 cgtcaggtag atgacctgga agagttggat atcggcatcc aggcgatggc ggcaattccg
     8401 gttggtgccg ctggcgaagg cattggcgaa agcgatgtcc gcgtcaattt tggcggtgtt
     8461 accttcttct ccggcgacca tctttatgcc gacaataccg ggattattct ttcagaagat
     8521 ccgctggata ttgaataagt atctgccatg cgttaaaagg ggaattaaat tccccttttt
     8581 tatacatgga cgtattcatg cag
//
LOCUS       DBFIFM010000181         8581 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.181, whole genome shotgun sequence.
ACCESSION   DBFIFM010000181 DBFIFM010000000
VERSION     DBFIFM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.181"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(229..492)
                     /gene="rpsT"
                     /locus_tag="N7C74_003779"
     CDS             complement(229..492)
                     /gene="rpsT"
                     /locus_tag="N7C74_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HGU2433572.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            595..819
                     /locus_tag="N7C74_003780"
     CDS             595..819
                     /locus_tag="N7C74_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HGU2433573.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGSQFLPVICAMTMT
                     MKSHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            827..1768
                     /gene="ribF"
                     /locus_tag="N7C74_003781"
     CDS             827..1768
                     /gene="ribF"
                     /locus_tag="N7C74_003781"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HGU2433574.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1811..4627
                     /gene="ileS"
                     /locus_tag="N7C74_003782"
     CDS             1811..4627
                     /gene="ileS"
                     /locus_tag="N7C74_003782"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HGU2433575.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPDEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFAHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYTEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4627..5121
                     /gene="lspA"
                     /locus_tag="N7C74_003783"
     CDS             4627..5121
                     /gene="lspA"
                     /locus_tag="N7C74_003783"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HGU2433576.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVTLVVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5209..5658
                     /gene="fkpB"
                     /locus_tag="N7C74_003784"
     CDS             5209..5658
                     /gene="fkpB"
                     /locus_tag="N7C74_003784"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU2433577.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5660..6610
                     /gene="ispH"
                     /locus_tag="N7C74_003785"
                     /gene_synonym="lytB"
     CDS             5660..6610
                     /gene="ispH"
                     /locus_tag="N7C74_003785"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HGU2433578.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6676..7590
                     /gene="rihC"
                     /locus_tag="N7C74_003786"
     CDS             6676..7590
                     /gene="rihC"
                     /locus_tag="N7C74_003786"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HGU2433579.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            7757..8578
                     /gene="dapB"
                     /locus_tag="N7C74_003787"
     CDS             7757..8578
                     /gene="dapB"
                     /locus_tag="N7C74_003787"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HGU2433580.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLDLNNL"
BASE COUNT         1948 a         2160 c         2440 g         2033 t
ORIGIN      
        1 ccacattctt tgtaggggag tctggagatt gagtagatat tcttgttcag aatgtatcag
       61 ccgatggttc tacgattctt aagccacgaa gagttcagat agtgcaacgg catgtctctt
      121 ttgactatct ggcaaccggc agtgtgttct ctcacgcatc acaaaagcag caggcataaa
      181 aaaacccgct tgcgcgggct ttttcacaaa gcttcagcaa attggcaatt aagccagttt
      241 gttgatctgt gcagtcaggt tagccttatg acgtgcagct ttgtttttgt ggatcagacc
      301 tttagcagcc tgacggtcca cgatcggttg catttcgtta aatgctttct gtgcagcagc
      361 tttgtcgcca gcttcgatag ctgcgtatac tttcttgatg aaagtacgca tcatagagcg
      421 acggcttgcg ttgtgcttac gagccttttc agactgaatg gcgcgcttct tagctgattt
      481 gatattagcc aaggtccaac tcccaaatgt gttctatatg gacaattcaa aggccgagga
      541 atatgccctt ttagccttct tttgtcaatg gatttgtgca aataagcgcc gttaatgtgc
      601 cggcactcgt tacgtagtga tggcgcagga ttctaccagc ttgcggggtg tgaatacagc
      661 ttttccgcga taaaaattgc agcaggcagt cagtttcttc ccgtgatttg cgccatgacc
      721 atgacaatga aaagccactt ctttctgatt tcggtactca atcgccggtt aaccttgacc
      781 gctgtacaag gtatactcgg acgattttca ctgttttgag ccagacatga agctgatacg
      841 cggcatacat aatctcagcc aggccccgca agaagggtgt gtgctgacta ttggtaattt
      901 cgacggcgtg catcgcggtc atcgcgcgct gttacagggc ttgcaggaag aagggcgcaa
      961 gcgcaactta ccggtgatgg tgatgctttt tgaacctcaa ccactggaac tgtttgctac
     1021 cgataaagcc ccggcacggc tgacccgact gcgggaaaaa ctacgttacc ttgcagagtg
     1081 tggcgttgat tacgtgctgt gcgtacgttt cgacaggcgt ttcgcggcgt taaccgcgca
     1141 aaatttcatc agcgatcttc tggtgaagca tttgcgggta aaattcctcg ccgtggggga
     1201 tgatttccgc tttggcgctg gtcgggaagg cgatttcttg ttattacaga aagctggcat
     1261 ggaatacggc ttcgatatca ccagtacgca aactttttgc gaaggtggcg tgcgtatcag
     1321 cagcaccgcc gtgcgtcagg cccttgcgga tgacaatttg gctctggcag aaagtttact
     1381 ggggcacccg tttgccatct ccgggcgtgt agtccacggt gatgaattag ggcgcactat
     1441 aggtttcccg acggcgaatg taccgctacg ccgtcaggtt tccccggtga aaggggttta
     1501 tgcggtagaa gtgctgggcc tcggtgaaaa gccgttaccc ggcgtggcaa acatcggaac
     1561 acgcccaacg gttgccggta ttcgccagca gctggaagtg catttgttag atgttgcaat
     1621 ggacctttac ggtcgccata tacaagtagt gctgcgtaaa aaaatacgca atgagcagcg
     1681 atttgcttcg ctggacgaac tgaaagcgca gattgcgcgt gatgaattaa ccgcccgcga
     1741 attttttggg ctaacaaaac cggcttaagc ctgttatgta atcaaaccga aacacggaac
     1801 cgagaatctg atgagtgact ataaatcaac cctgaatttg ccggaaacag ggttcccgat
     1861 gcgtggcgat ctcgccaagc gcgaaccggg aatgctggcg cgttggactg atgatgatct
     1921 gtacggcatc attcgtgcgg ctaaaaaagg caaaaaaacc ttcattctgc atgatggccc
     1981 tccttatgcg aatggcagca ttcatattgg tcactcggtt aacaagattc tgaaagacat
     2041 tatcgtgaag tccaaagggc tttccggtta tgactcgccg tatgtgcctg gctgggactg
     2101 ccacggtctg ccgatcgagc tgaaagtaga gcaagaatac ggtaagccgg atgagaaatt
     2161 caccgccgcc gagttccgcg ccaagtgccg cgaatacgcg gcgacccagg ttgacggtca
     2221 acgcaaagac tttatccgtc tgggcgtgct gggcgactgg tcgcacccgt acctgaccat
     2281 ggacttcaaa actgaagcca atatcatccg cgcgctgggc aaaatcatcg gcaatggtca
     2341 cctgcacaaa ggcgcgaagc cggtgcactg gtgcgtagac tgccgttctg cactggcaga
     2401 agcggaagtt gagtattacg acaaaacttc tccgtccatt gacgtcgctt tccaggcggt
     2461 cgatcaggat gcgctgaaag cgaaatttgg cgtaagcaac gttaacggcc caatctcgct
     2521 ggtgatctgg accactacgc cgtggactct gcctgcgaac cgcgcaatct ctattgcacc
     2581 tgatttcgac tatgcgctgg tgcagatcga cggtcaggcc gtgattctgg cgaaagatct
     2641 ggttgaaagc gtaatgcagc gtatcggcgt gaccgattac accattctcg gcacggtaaa
     2701 aggtgcggag cttgagctgc tgcgctttgc ccatccgttt atgggcttcg acgtcccggc
     2761 aatcctcggc gatcacgtta ccctggatgc cggtaccggt gccgttcaca ccgcgcctgg
     2821 ccacggcccg gacgactatg tgatcggtca gaaatacggc ctggaaaccg ctaacccggt
     2881 tggcccggac ggcacttatc tgccgggcac ttatccgacg ctggatggcg tgaacgtctt
     2941 caaagcgaac gacatcgtcg ttgcgctgct gcaggaaaaa ggcgcgctgc tgcacgttga
     3001 gaaaatgcag cacagctatc cgtgctgctg gcgtcacaaa acgccgatca tcttccgcgc
     3061 aacgccgcag tggttcgtca gtatggatca gaaaggtctg cgcgcgcagt ctctgaaaga
     3121 gatcaaaggt gtgcagtgga tcccggactg gggccaggcg cgtatcgagt cgatggtcgc
     3181 taaccgtcct gactggtgta tctcccgtca gcgtacctgg ggcgtaccga tgtctctgtt
     3241 cgtgcacaaa gacacggaag agctgcatcc gcgtaccctc gaactgatgg aagaagtggc
     3301 taaacgcgtt gaagttgatg gcatccaggc gtggtgggat cttgatgcga aagagatcct
     3361 cggcgatgaa gctgatcagt atgtgaaagt gccggatacg ctggatgtat ggtttgactc
     3421 cggctctact cactcttctg ttgttgacgt gcgcccggaa tttgccggtc acgctgcgga
     3481 catgtatctg gaaggttctg accaacaccg tggctggttc atgtcttctc tgatgatctc
     3541 caccgcgatg aagggcaaag cgccgtatcg tcaggtgctg acccacggct ttaccgtaga
     3601 tggtcagggc cgcaagatgt ctaaatccat cggcaacacc gtgtcgccgc aggatgtgat
     3661 gaacaaactg ggcgcggata ttctgcgtct gtgggtggca tcaaccgact acaccggtga
     3721 aatggccgtt tctgacgaga tcctgaaacg tgctgccgac agctatcgtc gtatccgtaa
     3781 caccgcgcgc ttcctgctgg caaacctgaa cggttttgat ccggcaaaag atatggtgaa
     3841 accggaagag atggtagtac tggatcgctg ggccgtaggt tgtgcgaaag cggcacagga
     3901 agacatcctc aaggcatacg aagcatacga tttccacgaa gtggtacagc gtctgatgcg
     3961 cttctgctcc gttgagatgg gttccttcta cctcgacatc atcaaagacc gtcagtacac
     4021 cgccaaagcg gacagcgtgg cgcgtcgtag ctgccagact gcgctgtatc acatcgcaga
     4081 agcgctggtt cgctggatgg caccaatcct ctccttcacc gctgatgaag tgtggggcta
     4141 cctgccgggc gaacgtgaaa aatacgtctt caccggcgag tggtacgaag gtctgtttgg
     4201 tctggcagac agtgaagcaa tgaacgatgc gttctgggac gagctgttga aagtgcgtgg
     4261 cgaagtgaac aaagtcattg agcaggcgcg tgccgacaag aaagtgggtg gctcgctgga
     4321 agcggcagta accttgtata cagaaccgga actggcggcg aaactgaccg cattgggcga
     4381 tgaattacga tttgtcctgt tgacctccgg cgctaccgtt gcagactata acgacgcacc
     4441 tgctgatgct cagcagagcg aagtgctcaa agggctgaaa gtcgcgttga gtaaagccga
     4501 aggtgagaag tgcccacgct gctggcacta cacccaggat gtcggcaagg tggcggaaca
     4561 cgcagaaatc tgcggccgct gtgtcagcaa cgtcgccggt gacggtgaaa aacgtaagtt
     4621 tgcctgatga gtcaatcgat ctgttcaaca gggctacgct ggctgtggct ggtggtagtc
     4681 gtgctgatta tcgatctggg cagcaaatac ctgatcctcc agaactttgc tctgggggat
     4741 acggtcccgc tgttcccgtc gcttaatctg cattatgcgc gtaactatgg cgcggcgttt
     4801 agtttccttg ccgatagcgg cggctggcag cgttggttct ttgccggtat tgcgattggt
     4861 attagcgtta ccctggttgt gatgatgtat cgctcgaagg ccacgcagaa gctaaacaat
     4921 atcgcctacg cgctgattat tggcggcgcg ctgggcaacc tgttcgaccg cctgtggcac
     4981 ggcttcgttg tcgatatgat cgacttctac gtcggcgact ggcacttcgc caccttcaac
     5041 ctcgccgata ctgccatctg tgttggtgcg gcactgattg tgctggaagg ttttttgcct
     5101 tctaaagcga aaaaacaata ataaaccctg ccggataagg cctacagatt gctgcgaaat
     5161 cgtaggccgc atccggcaaa aatcctcaaa atataagagc aaacctgcat gtctgaatct
     5221 gtacagagca atagcgccgt cctggtgcac ttcacgctaa aactcgacga tggcaccacc
     5281 gccgagtcta cccgcaacaa cggtaaaccg gcgctgttcc gcctgggcga tgcttctctt
     5341 tccgaagggc tggagcaaca cctgctgggg ctgaaagtgg gcgataaaac cactttctcg
     5401 ctggagccag atgcggcgtt tggcgtgccg tcaccggacc tgattcagta cttctcccgc
     5461 cgtgagttta tggatgcagg cgagccagaa attggcgcaa taatgctttt taccgcaatg
     5521 gatggcagtg agatgcctgg cgtgatccgc gaaattaacg gcgactccat taccgtcgat
     5581 ttcaaccatc cgctggccgg gcagaccgtt cattttgata ttgaagtgct ggaaatcgat
     5641 ccggcactgg aggcgtaaca tgcagatcct gttggccaac ccacgtggtt tttgtgccgg
     5701 ggtagaccgc gctatcagca ttgttgaaaa cgcgcttgcc atttacggcg caccgatata
     5761 tgtccgtcac gaagtggtgc ataaccgcta cgtggtcgat agcctgcgcg agcgtggagc
     5821 tatctttatt gagcagatca gcgaagtgcc ggacggcgcg atcctgatct tctccgcaca
     5881 tggtgtttct caggcggtac gtaacgaagc gaaaagccgt gatttgacgg tattcgacgc
     5941 cacctgtccg ctggtgacca aagtgcatat ggaagtcgcc cgcgccagcc gtcgtggcga
     6001 agagtctatt ctcatcggtc acgccgggca cccggaagtg gaagggacga tggggcagta
     6061 cagcaaccct gaagggggaa tgtatctggt cgaatcgcct gacgatgtgt ggaaactgac
     6121 ggtcaaaaac gaagagaagc tctcctttat gacccaaacc acgctgtcgg tagatgacac
     6181 gtctgatgtg atcgacgcgc tgcgtaaacg cttcccgaaa attgtcggtc cgcgcaaaga
     6241 tgacatctgc tacgccacga ctaaccgtca ggaagcggta cgcgccctgg cagaacaggc
     6301 ggaagttgtg ttggtggtcg gttcgaaaaa ctcctccaac tccaaccgtc tggcggagct
     6361 ggcccagcgt atgggcaaac gcgcgttttt gattgacgat gcgaaagata tccaggaaga
     6421 gtgggtgaaa gaggttaaat gcgtcggcgt gactgcgggc gcatcggctc cggatattct
     6481 ggtgcagaat gtggtggcac gtttgcagca gctgggtggt ggtgaagcca ttccgctgga
     6541 aggccgtgaa gaaaatattg ttttcgaagt gccgaaagag ctgcgtgtcg atattcgtga
     6601 agtcgattaa gtcattagca gcctaagtta tgcgaaaatg ccggtcttgt tatcggcatt
     6661 ttttatggag aaaacatgcg cttacctatc ttcctcgata ctgaccccgg cattgacgat
     6721 gccgtcgcca ttgccgccgc gatttttgcc cccgaactcg acctgcaact gatgaccacc
     6781 gtcgcgggta atgtctcggt tgagaaaact acccgtaacg ccttgcaact gctgcatttc
     6841 tggaatgcgg agattccgct cgcccaggga gccgctgtgc cgctggtacg cgcaccgcgt
     6901 gatgcagcat ctgtgcacgg cgaatcggga atggctggct acgactttgt tgagcacaac
     6961 cgaaagccac tcgggatacc ggcgtttctg gcgattcggg atgccctgat gcgtgcacca
     7021 gagcccgtta ccctggtggc catcggcccg ttaaccaata ttgcgctgtt actttcacaa
     7081 tgcccggaat gtaaaccgta tattcgccgt ctggtgatca tgggtggttc tgccggacgc
     7141 ggcaactgta cgccaaacgc cgagtttaat attgctgccg atccagaagc tgctgcctgt
     7201 gtcttccgca gtggtattga aatcgtcatg tgcggtttgg atgtcaccaa tcaggcaata
     7261 ttaactcctg actatctctc tacactgccg cagttaaacc gtaccgggaa aatgcttcac
     7321 gccctgttta gccactaccg tagcggcagt atgcaaagcg gcttgcgaat gcacgatctc
     7381 tgcgccatcg cctggctggt gcgcccggac ctgttcactc tcaaaccctg ttttgtggca
     7441 gtggaaactc aaggcgaatt tacctcaggc acgacggtgg ttgatatcga cggttgcctg
     7501 ggcaaaccag ccaatgtaca ggtggcattg gatctgaatg taaaaggctt ccaacagtgg
     7561 gtggctgagg tgctggctct ggtgccgtaa cctgtcacat gttatcggca tgcagtcatt
     7621 catcgactca tgcctttcac tgatatccct ccctgtttat cattaatttc taattatcag
     7681 cgtttttggc tggcggcgta gcgatgcgct ggttactctg aaaacggtct atgcaaatta
     7741 acaaaagaga atagctatgc atgatgcaaa catccgcgtt gccatcgcgg gagccggggg
     7801 gcgtatgggc cgccagttga ttcaggcggc actggcatta gaaggcgtgc agctgggcgc
     7861 ggcgctggag cgcgaaggat cttctttact gggcagcgac gccggtgagc tggctggtgc
     7921 agggaaaaca ggcgttaccg tgcaaagcag tcttgatgcg ataaaagatg attttgatgt
     7981 gtttatcgat tttacccgtc cggaaggtac gctgaaccat ctcgcttttt gtcgccagca
     8041 tggcaaaggg atggtgatcg gcactacggg gtttgacgaa gccggaaaac aggcaattcg
     8101 tgacgccgcc gccgatattg cgattgtctt tgccgccaat tttagcgttg gcgttaacgt
     8161 catgcttaag ctgctggaga aagcagccaa agtgatgggt gactacaccg atattgaaat
     8221 tattgaagca catcatagac ataaagttga tgcgccgtca ggcaccgcac tggcaatggg
     8281 agaggcgatc gcccacgccc ttgataaaga tctgaaagat tgcgcggtct acagtcgtga
     8341 aggccatacc ggtgaacgtg tgcctggtac cattggtttt gccaccgtgc gtgcaggtga
     8401 catcgttggc gaacataccg cgatgtttgc cgatattggc gagcgtctgg agatcaccca
     8461 taaggcgtcc agccgcatga catttgctaa cggtgcggta agatcggctt tgtggttgag
     8521 tggtaaggaa aatggtcttt ttgatatgcg agatgtgctt gatctcaata atttgtaacc
     8581 a
//
LOCUS       DBFIFM010000182         8572 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.182, whole genome shotgun sequence.
ACCESSION   DBFIFM010000182 DBFIFM010000000
VERSION     DBFIFM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.182"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..674)
                     /locus_tag="N7C74_003788"
     CDS             complement(<1..674)
                     /locus_tag="N7C74_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HGU2433581.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNFRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAV"
     gene            complement(685..1686)
                     /gene="ltaE"
                     /locus_tag="N7C74_003789"
     CDS             complement(685..1686)
                     /gene="ltaE"
                     /locus_tag="N7C74_003789"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HGU2433582.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKDAWEFTRERN
                     LALHVDGARIFNAVVAYGSELKELTQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(1723..3441)
                     /gene="poxB"
                     /locus_tag="N7C74_003790"
     CDS             complement(1723..3441)
                     /gene="poxB"
                     /locus_tag="N7C74_003790"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HGU2433583.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEID
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(3574..4542)
                     /gene="hcr"
                     /locus_tag="N7C74_003791"
     CDS             complement(3574..4542)
                     /gene="hcr"
                     /locus_tag="N7C74_003791"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HGU2433584.1"
                     /translation="MTMPTNQCPWRMQVHHIKQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAQEFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(4554..6206)
                     /gene="hcp"
                     /locus_tag="N7C74_003792"
                     /gene_synonym="priS"
     CDS             complement(4554..6206)
                     /gene="hcp"
                     /locus_tag="N7C74_003792"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HGU2433585.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(6350..7249)
                     /gene="lysO"
                     /locus_tag="N7C74_003793"
     CDS             complement(6350..7249)
                     /gene="lysO"
                     /locus_tag="N7C74_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HGU2433586.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(7707..8402)
                     /gene="aqpZ"
                     /locus_tag="N7C74_003794"
     CDS             complement(7707..8402)
                     /gene="aqpZ"
                     /locus_tag="N7C74_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HGU2433587.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
BASE COUNT         2123 a         2338 c         2234 g         1877 t
ORIGIN      
        1 accgccataa aatgttcaaa ctgttgctga taactgagca cctctggtcc ggcggcttcg
       61 aagatgcggt gttcgttggc tggatggtct aacagcgcca ccagatagtg cagcaagttt
      121 tccagcgcga tgggcgtggt gcgtgaacgt acccagcgtg gcggcgttaa cactggcagg
      181 ttgtagacca tatcgcgcat gacttcgaac gctgctgaac ctgcaccaac gattattccg
      241 gcacgcaatt cggtcactgg tacattcgct tcacgaagaa tgtccgccgt agcctgacgc
      301 gcgcgcagat ggtccgactg ctcatgtggc ggggcctgca acgaactgag aaagattaat
      361 tgcttaactg gtacttcacg tagcgcatcg cggaagttga gagccacctg gcgctcctga
      421 gcgataaaat cgccgccttc gcccatgcta tgcaccagaa aatagaccgt atcgatatcc
      481 tgtaacaggg ccggaaggtt atccggccag ctgagatcga ctttatggca actgacgttt
      541 gccagttgca gctttgcaag cctgtcgaca tgacgtgccg ccgccaggat ctgatgccct
      601 tgctggctga gtgtgcgcac cagatgctga ccaatgtagc cactggcacc aagaactaaa
      661 atgcgttgcg gcacgtctcc ctccttaacg cgccaggaat gcgcgccagt gggcggcgac
      721 ttctgccagt tgttcgcgcg agacgtcaag atgggtcacc aggcggacaa tcggcgaggc
      781 gttaatcagt acgtttctcg ctttcatgta ttcgcctaac gcggcagcat tttcttcgcc
      841 gacgcgaaca aacagcatat tagtgtcctg acgcatcaca tccgcgcctg cttcacgcag
      901 ttgctccgcc atccaggcgg cgttgtcgtg atcttcctgc aaccgtgcga cgttattttt
      961 cagcgcatac atcccggcgg cagccagaat gccggactgg cgcatcccgc cacctgtcat
     1021 tttccgccag cgaatggcac gtttaatgta atcacgatta ccgacgagta atgaaccgac
     1081 tggcgtccca agacctttcg acaggcaaat ggtgaacgaa tcacaatatt gcgtgagctc
     1141 tttcagttcg ctgccgtaag ccaccacggc attaaagatg cgcgcaccgt caacatgcag
     1201 cgccagattg cgctcgcggg taaattccca tgcatcttta aggtattcac gcggcaacac
     1261 tttgccgttg tgggtgtttt ccagactgag taatttggtg cgggcgaaat ggatatcgtc
     1321 gggtttgatt ttcatcgcca ctttatccag cggtagcgtg ccgtcggcag ccgcgtcgat
     1381 gggttgcggt tgaatactgc ccagcaccgc cgcgccaccg gcttcaaaca gatagttatg
     1441 cgcggcctga ccgacaatat actcttcgcc gcgttcgcag tgactgagca gagcgaccag
     1501 gttggcctga gtgccggtag gcagaaaaat ggcggcttct ttaccggaaa gctctgctgc
     1561 atagtcctgc agagcattaa cggtagggtc gtctccgtaa acgtcgtccc caaccggggc
     1621 ggccatcatc gcttcgagca tggcgcggct cggtcgggtt acggtatcac tgcgtaaatc
     1681 aatcatggca tgtccttatt atgacgggaa atgccaccct ttttacctta gccagttcgt
     1741 tttcgccagt tcgatcactt catcaccgcg tccgctgatg attgcgcgca gcatatacag
     1801 gctgaaacct ttggcctgtt cgagtttgat ctgcggtgga atggctaact cttctttggc
     1861 gaccaccaca tccaccaata ccggaccgtc gatggagaag gcgcgttgca gggcttcatc
     1921 gatttcagag gctttttcta cacgaatacc cgtaattccg cacgcttcgg caatgcgggc
     1981 aaagttggtg tcgtgcagct cagtaccgtc tgtcaggtag cctccggctt tcatctccat
     2041 cgccacaaag cccagcacgc tgttattaaa gacgataatt ttcaccggca gtttcatctg
     2101 cactaccgag aggaaatcgc ccatcaacat gctaaaaccg ccatcgccgc acatggcgac
     2161 cacctgacgt tccggctcgg ttgcctgcgc gcccagcgcc tgcggcatgg cgttagccat
     2221 cgaaccgtgg ttaaacgaac ctaacaggcg acgcttgccg ttcattttca gataacgcgc
     2281 cgcccacacc gttggcgtgc cgacgtcaca ggtaaagatg gcgtcatcgg cggcaaaatg
     2341 actaatttgc tgcgccagat attgcgggtg aatggctttc tcgctcggtt tagctaaatc
     2401 gtccagccct ttgcgggcat cgcggtaatc ttccagcgct ttatccagaa acttgcgatc
     2461 ggctttttct tccaccaatg gcagcaatgc acgcagggtc gacttgatat cgccgaccag
     2521 cgccatatcc accttgctgt gtgcgccgat gctggctggg ttgatatcaa tctgaataat
     2581 tttggcatcg gtcgggtaga acgcacggta ggggaactga gtaccgagta gcactaacgt
     2641 gtcggcgttc atcatggtat ggaaacctga cgagaatccg attaacccgg tcattccaac
     2701 atcatacgga ttatcgtatt cgacatgctc tttaccgcgt agggcatgaa caataggtgc
     2761 tttaattttc ccggcaaact caactaactc tttatgcgcc cccgcgcagc cgctgccaca
     2821 catcagagcg atattgctgg aataacgcag cagttgcgcc agtttgcgta actcttcttc
     2881 ttccggcgtc acgactggct gtggcgcatg ataccagtgg gtagttgccc cttctggcgc
     2941 aggttttaac gccacgtcgc ccggtaacac gacaacggaa acgccacggt taagcaccgc
     3001 tttgcgcatg gcaatcgcca gcacttgtgg gatctgctcc gggctggaaa ccagctcgca
     3061 atagtgacta cattcgcgga atagctcttg tgggtgggtt tcctggaaat agccgctgcc
     3121 aatttcgctg gaaggaatat gagcggcaat cgccagtacc ggaacgtgat tgcggtggca
     3181 atcgaacagg ccgttgatta agtgcaggtt gccggggccg cacgatccag cacagaccgc
     3241 cagttctcct gaaagttgtg cttcagcgcc agcggcaaac gccgccactt cttcatggcg
     3301 ggtggacatc cactcgatgg tgcccatgcg attaagactg tcgctaagac cgttcagaga
     3361 gtcgcccgtg actccccaga tgcgtttcac gcctgccgat tcgagtgttt tggcgatata
     3421 agctgcaacc gtttgtttca tggttctcca tctcctgaat gtgataacgg taacaagttt
     3481 agttcatctg acggaggggg aagggatggg agagaaagga ggcactaacg gttaaatagc
     3541 ccgatgaaag gaatatcatc gggcataagg cgattatgcg agaaccaaat ccccctgcgg
     3601 atggcaggag caggccagta cgtaaccttc agcgatttcg gcgtcggtca gcgtcattgt
     3661 actgctcacc gtatattcac cggaaaccac tttcgtctta cagcagccgc aaacacccgc
     3721 gcggcaggcg gcgacaaccg gaacgttatt gctttccagc gcctccagca gcgtggtgcc
     3781 aacaggagcg taaaattctt gcgccggttg cagtttggtg aatttcagac cgctggtcgc
     3841 agcttccgct actggggtga agaatttctc tttaaagaaa cgcgtcacgc cgagtgcttt
     3901 cacttcctgc tctacccaat ccatatacgg agccgggccg caggtcatca cggtacgtga
     3961 agctaagtca ggaacgcgag tcaggagttc gcgagtgaga cgaccagcga taaagccttc
     4021 ggtaacgtta ttttccgcca ccagcgttac cggatagtta cgccactcat cggcgaaaat
     4081 aacatcctgc ggcgtacgca cgttgtagat cacccgcaca tcggcctgtg gacggttctt
     4141 cgccagccag cgacgcatcg acataatcgg cgtgacgccg cagcctgcag ccagcaacag
     4201 gaatttatct tctgctttat cgtcgcaggt aaattccccc atcgcgtctg aaagccagag
     4261 ataatcaccg cgttttacat cgcgggtcag ccactgggag ccgacaccgt catcaatccg
     4321 ccgcacggtc agggtgatat attcactcac gcctggcgtg gaggaaatgg tgtaagcacg
     4381 cagcgtttcc gctgagttac gcacgctgac cagtgcatat tgcccggcgc ggtatgggta
     4441 gtaatcgtgg caaatcaggg aaatcgtcca cacatccggc gtttcttgct taatgtgatg
     4501 aacctgcatc cgccacgggc attgattcgt tggcatcgtc atcgacaaac tccttacgcg
     4561 ctcaacagtt gcttcatgtc ttcttcaaca gtggtgatag aacgcaggcc gaatttctcg
     4621 ttcagcaccg ccagcagatc tggtgtcagg aaaccaggtg cagtcgggcc ggtgacgata
     4681 tttttcacgc ccagagaaag cagcgtcagc agaatgacaa tcgctttctg ttcaaaccag
     4741 gagagcacca gcgacagcgg cagatcgttg acaccgcagc ccagtttttc tgccagcgta
     4801 accgccagaa taattgctga gtaagcatcg ttacactgac cggcatccac cagacgaggc
     4861 agaccttcga tatcgccaaa ctcaagtttg ttaaagcgat atttaccaca ggcgagagtc
     4921 aggatcaggc agtcatctgg cacgctggtg gcgaaatcgg tgaagtagtg acgttcgccg
     4981 cgtgcgccgt cacagccacc aagcaggaag atatgacgca gtttttcacg gctcaccaga
     5041 tcaattagcg tatccgctgc gccaagcagc gtctggcggc caaagcccac ggtgatcagg
     5101 tgcggaattt cgctgtatgg gaagcctgcc atttgttgcg cctgggcaat gaccgcagag
     5161 aaatcttcgc catccagatg acgcacgcca ggccagccaa cgatgctgcg ggtccagata
     5221 cgatcgtcat aagcgcctac ggttgggtcg atgatgcagt tcgaggtcat cacgatgggg
     5281 ccagggaaac gagcgaactc cacttgctga ttctgccagc cgctgccgta gttaccgacc
     5341 agatgcttga atttacgcag ctccgggtag ccatgcgcag gcagcatttc gccgtgggtg
     5401 tagacattaa cgcccgtgcc ttcggtctgt tccagcaggt tgtagagatc tttgagatcg
     5461 tgaccggaaa tcaggatgca tttacccgcc gtcgctttga cgttgacctg ggttggcgtc
     5521 gggtgaccgt atttaccggt ttcgcctgca tccagaatgc tcatcacttt gaagttcatc
     5581 tggccgattt ccattgaaca ctcaagaagc gcgttcatat cggcaggcca ggtccccagc
     5641 cacgccatga ttttatgata ctgggcataa atatcgttgt cgtattgacc gagaacgtgt
     5701 gcgtgttcca tataggccgc cgcacctttc aggccataca ggcacagcag acgcaggccg
     5761 agaatgtttt cgccaatcgc cgctttatct ttgttagggg taaattctgc tgcctgacgt
     5821 tgcagttcgc cgagatcgtc gctcaccagt tgcaggtcag ccatcgggtt atcgacgcgg
     5881 gcgttggcat ctacagccag gcattgtgct ttcagcgcct cgcgcaaggc aatcgcttca
     5941 cgagcgtagc cgacaatacg cggagaatcg aagttaacgt tggtcagggt tgagaaaaag
     6001 gcacgtggcg cgaagctgtc gacatcgtgg ttgatgatgc cgtattcacg cgcttttacc
     6061 gcccaggcag aaagcccttg cagcgccgcg atgagtaaat cctgaaggtc agaagtttcc
     6121 gccgttttac cacacatccc ctgcgcgtat gagcagccgt ttcctgccgg agtacggata
     6181 gtttgttcac attgcacaca aaacatgatc acacctttta aagttatatt taatatacat
     6241 gtttaaggtt aagacgctta acgcggggat aaaagggatt tttcatgcaa ctttaaggga
     6301 gattgattta gcgcaatttt ggcggcaggg atctaccgcc agagaggtat tacgcggaga
     6361 aaaaggcgat gaggatcggc actaacaggc taagaataaa accgtgaaca attgccgccg
     6421 ggaccatatc cagcccgcca gtacgttgaa gaacgggcag ggtgaaatcc atcgacgtcg
     6481 caccgcataa gcccagtgcg gtagagcggc tgcggcgaat cagcccaggg atcaacataa
     6541 ttgcaatcag ctcacgggcc agatcattaa aaaacgccgc gctcccgatt accggaccaa
     6601 aagattcggt caataaaata ccggaaagag aataccagcc gaaaccagag gccattgcca
     6661 gcgcggtatt gatgggtaga tcaagaataa aggcgttaat taaaccacca attaatgaac
     6721 tggcaaccac caccacggcg acaatcattc cccggcgatt aaggacaatc tgctttaagg
     6781 tcatgccatt attgcgcaac tgaataccga cgaggaaaag tagcagaatt aacgtgtatt
     6841 cactggcttc ggtcgcgtgt tgtaagaaag ccagtccact caggccaatg gcaaaaccaa
     6901 tcactactac gccgcacagt tttagcgact caagcgccat cgcaatacgc gacgggagtt
     6961 tttcttgctg atggtggttg cgccacggca gaccgcgctc cagccacata agggcggcaa
     7021 tattacacag taaaataacg gtaatactga cggcagaata atgcagaatc gccaacaggt
     7081 tactggcgag gttatcgaga aacgccagac tgatacccat aaaaaagaga ataaggtaaa
     7141 ccatccagct taatagctga ttaataactt ttaacgcagc ttgttggcga agcggaatga
     7201 ggtaacccac aatcagggga accagaatga ttaacaaccc agaaaacatg aaaaaccagc
     7261 ccttgcaaag aaagaagtcg aaatgcgcga tgacacacta ctgaaagcgg aaggacgagt
     7321 aaagttgcaa ttaaaaggaa atgttatgca tcagaggggg atttttaatt ctatatgagt
     7381 gtttagcatt gttgcgctcc tttcatgtgc aatgccatat aaagccactg gcgcagggag
     7441 cgcgcagggg gcggccagtc gccgccgccc cctgcacccc cgggctctgg cgaacaaaat
     7501 cgccgctgcg cggtaccttc agcttatccc cttcggcaac cgggtcgggc gcgaggtaac
     7561 gtcctgtaag acgcgccctc agcccacatc catgtgggct gccccggcct tcggggaacg
     7621 cttcagcgat tttgacgcca ccaatacccg tgcggtttgt aatgtcgaag ataaaacaac
     7681 cagggcagat aaaatgccaa tgctaattaa ttacgctttt ccagcagagt ccggtaaatc
     7741 agaccaccga taatgccacc gacaattggc accacccaga agaaccacag ttgttctaat
     7801 gcccagccgc cctggaagat ggcaaccgcg gtgctgcgcg ccgggttaac agaagtgtta
     7861 gtcaccggaa tactgattaa gtgaatcagg gttaaggcca gaccaatagc gatcggcgca
     7921 aaacctgccg gagcgaattt gtcggttgca ccgtggatca ctaacaggaa gcccgcactc
     7981 aataccagtt caactaccag cgcggaaagc atggaataac cgcctggtga atgctcgcca
     8041 taaccgttag aagcaaaacc gctggctgcc gcgtcaaaac ccgttttacc actggcaatt
     8101 aaatacagca gcgctgccgc aacaataccg ccgacaacct gggcaattac gtagccaacg
     8161 acttcttttg ccggaaaacg tccgccagcc cataaaccaa tagtgaccgc cgggttaaaa
     8221 tgaccaccag aaatatgacc aacagcaaag gccatcgtca gaacggtcag accgaacgcc
     8281 aacgccacgc cggcaaaacc aatgcctaat tccgggaagc ctgcggccag tacagcacta
     8341 ccacagccac caaaaacaag ccagaaagta ccaaaacatt cagctgctaa ttttctgaac
     8401 atatccacct caattaaaaa ttgaccctgt gaaaaatatg gtcgttttat agggtcgtcg
     8461 taaaaaagtg acgacggaaa taatgcgcgg ctattttaaa aacgaaggcg agtcattcac
     8521 cagataaata aatccagtaa atttgattta gggcaacagc gggttgcccc at
//
LOCUS       DBFIFM010000183         8567 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.183, whole genome shotgun sequence.
ACCESSION   DBFIFM010000183 DBFIFM010000000
VERSION     DBFIFM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.183"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            104..1207
                     /gene="ompC"
                     /locus_tag="N7C74_003795"
     CDS             104..1207
                     /gene="ompC"
                     /locus_tag="N7C74_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HGU2433588.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1319..2374
                     /gene="apbE"
                     /locus_tag="N7C74_003796"
     CDS             1319..2374
                     /gene="apbE"
                     /locus_tag="N7C74_003796"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HGU2433589.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2448..3512
                     /gene="ada"
                     /locus_tag="N7C74_003797"
     CDS             2448..3512
                     /gene="ada"
                     /locus_tag="N7C74_003797"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HGU2433590.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYHKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3512..4162
                     /gene="alkB"
                     /locus_tag="N7C74_003798"
     CDS             3512..4162
                     /gene="alkB"
                     /locus_tag="N7C74_003798"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HGU2433591.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4238..5881
                     /gene="yojI"
                     /locus_tag="N7C74_003799"
     CDS             4238..5881
                     /gene="yojI"
                     /locus_tag="N7C74_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HGU2433592.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPSWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELRNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6099..7745
                     /gene="mqo"
                     /locus_tag="N7C74_003800"
     CDS             6099..7745
                     /gene="mqo"
                     /locus_tag="N7C74_003800"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HGU2433593.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(7895..8383)
                     /gene="eco"
                     /locus_tag="N7C74_003801"
     CDS             complement(7895..8383)
                     /gene="eco"
                     /locus_tag="N7C74_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HGU2433594.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDTGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
BASE COUNT         2061 a         2142 c         2314 g         2050 t
ORIGIN      
        1 tgcatttttg gggagaatgg acttgccgac tgattaatga gggttaatca gtatgcagtg
       61 gcataaaaaa gcaaataaag gcatataaca gagggttaat aacatgaaag ttaaagtact
      121 gtccctcctg gtcccagctc tgctggtagc aggcgcagca aacgctgctg aagtttacaa
      181 caaagacggc aacaaattag atctgtacgg taaagtagac ggcctgcact atttctctga
      241 cgacaagtct gtagatggcg accagaccta catgcgtctt ggcttcaaag gtgaaactca
      301 ggttactgac cagctgaccg gttacggcca gtgggaatat cagatccagg gcaacagcgc
      361 tgaaaacgaa aacaactcct ggacccgtgt ggcattcgca ggtctgaaat tccaggatgt
      421 aggttctttc gactacggtc gtaactacgg cgtagtttac gacgtaactt cctggaccga
      481 cgttctgcca gaattcggtg gtgacaccta cggttctgac aacttcatgc agcagcgtgg
      541 taacggtttc gcgacctacc gtaacactga cttcttcggt ctggttgacg gcctgaactt
      601 tgctgttcag taccagggca aaaacggtag cgtaagcggc gaaggcatga ctaacaacgg
      661 tcgtgaagca ctgcgtcaga acggcgacgg cgttggcgga tctatcactt atgattacga
      721 aggcttcggt atcggtgctg cagtttccag ctccaaacgt actgatgatc agaacagccc
      781 gctgtacatc ggtaacggcg accgtgctga aacctacact ggtggtctga aatacgacgc
      841 taacaacatc tatctggctg ctcagtacac ccagacctac aacgcaactc gcgtaggttc
      901 cctgggttgg gcgaacaaag cacagaactt cgaagctgtt gctcagtacc agttcgactt
      961 cggtctgcgt ccgtccctgg cttacctgca gtctaaaggt aaaaacctgg gtgtcatcaa
     1021 tggtcgtaac tacgacgacg aagatatcct gaaatatgtt gatgttggcg cgacctacta
     1081 cttcaacaaa aacatgtcca cctatgttga ctacaaaatc aacctgctgg acgacaacca
     1141 gttcactcgt gacgctggca tcaacactga taacatcgta gctctgggtc tggtttacca
     1201 gttctaatct cgattgatat cgaacaaagg gcctgcgggc ccttttttca ttgttttcag
     1261 cgtacaaact cagttttttg gtgtactctt gcgaccgttc gcatgaggat aatcacgtat
     1321 ggaaataagc tttacccgcg tggcactgct ggctgccgcg ctcttctttg ttggttgcga
     1381 tcaaaaacca caacccgcca aaacccacgc tactgaagtt accgttcttg aaggcaaaac
     1441 tatgggtacc ttctggcgtg ccagcatccc gggcattgac gccaaacgca gcgccgaact
     1501 taaagaaaag attcagaccc agctggacgc cgacgatcag ctgctttcga cctataaaaa
     1561 agattccgcg ctgatgcgct ttaacgactc gcaaagttta tcgccgtggc cggtaagtga
     1621 agcgatggcc gatatcgtca ccacctcgct acgcattggc gcgaagaccg atggcgcgat
     1681 ggatataacc gtcgggccgc tggtgaatct gtggggtttc ggtccggaac aacagccggt
     1741 tcaaattccg agccaggaac agatcgacgc gatgaaagcc aaaactggtt tacagcacct
     1801 tacggtcatt aatcagtcgc atcagcaata tctgcaaaaa gacctgccgg atttatatgt
     1861 cgatctctcc accgtcggcg aaggttatgc ggcggatcac ctggcacgct tgatggagca
     1921 ggaagggatt tcccgctatc tggtgtcggt gggcggcgcg ctgaacagcc gtggtatgaa
     1981 cggtgaaggc ctgccgtggc gggtagcgat tcaaaaacca accgataaag aaaacgcggt
     2041 tcaggccgtg gtggatatca acggtcatgg gattagcacc tctggcagct accgtaacta
     2101 ttacgaactg gacggcaaac gtctttccca tgttatcgat ccgcaaaccg ggcgtcccat
     2161 cgaacacaat ctggtatccg tgacggtgat tgcaccgacg gcgctggaag ccgacgcctg
     2221 ggatacaggt ttgatggtac tcgggccgga gaaagccaaa gaagttgttc gccgggaagg
     2281 gctggcggtt tatatgatca ccaaagaagg cgatagcttt aaaacctgga tgtcaccaca
     2341 gtttaaaagc ttccttgtca gcgaaaaaaa ttaaagcgca agattgttgg tttttgcgtg
     2401 atggtggccg ggcagactaa aggctatcct taaccaggga gctgattatg aaaaacgcca
     2461 catgcttaac tgacgatcaa cgctggcaat ctgtcttagc ccgcgacccg aatgccgacg
     2521 gcgaattcgt tttcgccgtg cgcaccacag gcatcttttg ccgtccgtct tgccgcgcca
     2581 gacatgcttt gcgggaaaac gtctccttct acgcaaatgc cagcgaggca ctcgccgccg
     2641 gatttcgccc ctgcaaacgc tgccaacctg ataaagccaa tgcccagcaa catcggttgg
     2701 ataaaatcac ccacgcgtgt cgactgctgg aacaggaaac gcctgtaacg ttggaagcct
     2761 tagccgacca ggtggcgatg agtccatttc atctacatcg gttgtttaaa gcgactaccg
     2821 gaatgacgcc taaagcctgg caacaggcct ggcgcgctcg ccgtttgcgc gaatcgctgg
     2881 cgaaagggga gagcgtgacg acgtctattc ttaacgccgg attccccgac agcagcagtt
     2941 actaccacaa agccgatgaa acgctgggca tgacggctaa acaattccgt cacggtggcg
     3001 aaaatctggc ggtgcgttac gcgctggctg attgtgagct gggtcgttgc ctggtggcag
     3061 aaagcgagcg ggggatttgc gcgatattgc tgggcgatga tgacgcgaca ttaatcagcg
     3121 agttgcagca gatgtttccc gctgccgaca acgcgcctgc cgatctgacg tttcagcaac
     3181 atgtgcgtga agtgatcgcc agcctcaatc aacgcgatac tccgctgacg ttaccgctgg
     3241 atattcgcgg cactgctttt cagcaacaag tctggcaggc actgcgcacg ataccttgcg
     3301 gtgaaaccgt cagttatcag caactggcta acgccatcgg caaaccgaaa gcggtacggg
     3361 ccgttgccag cgcctgtgcc gccaacaagc tggctatcgt aattccctgt catcgagtgg
     3421 tccgtggtga tggcacactt tccggttacc gctggggcgt gtcgcgtaaa gcgcaactgc
     3481 tgcgccgcga agctgaaaat gaggagaggt aatgttggat ctgtttgccg atgctgaacc
     3541 gtggcaagag ccactggcgg ctggtgcggt aattttacgg cgttttgctt ttaacgctgc
     3601 agagcaacta atccgcgata ttaatgacgt tgccagccag tcgccgtttc gccagatggt
     3661 cacccccggg ggatatacca tgtcggtggc gatgaccaac tgtgggcatc tgggctggac
     3721 gacccatcgg caaggttatc tctattcgcc cattgatccg caaacaaata aaccgtggcc
     3781 cgccatgccg cagagttttc ataatttatg tcaacgtgcg gctacggcgg cgggctatcc
     3841 agatttccag ccagatgctt gtcttatcaa ccgctacgct cctggcgcga aactgtcgct
     3901 gcatcaggat aaagacgaac cggatctgcg cgcgccaatt gtttctgttt ctctgggctt
     3961 acccgcgatt tttcaatttg gtggcctgaa acgtaatgat ccgctcaaac gtttgttgtt
     4021 ggaacatggc gatgtggtgg tatggggcgg tgaatcgcgg ctgttttatc acggtattca
     4081 accgttgaaa gcggggtttc atccactcac caccgactgc cgctacaacc tgacattccg
     4141 tcaggcaggt aaaaaagaat aaaaataaga attattattg ctgtgcgcgc gaagatgttt
     4201 aaactgcggg ctgtaattca ttgtccgggt tttctgcatg gaacttcttg tacttgtctg
     4261 gcggcagtat cgctggccat ttatcagtgt gatggcgcta agcctcgcca gtgcggcttt
     4321 aggcattggc ttaattgctt ttatcaatca gcgccttatc gaaacggcgg ataccagtct
     4381 gctggtgttg ccggagtttc tgggattatt gctgctgttg atggcagtca ctctcggatc
     4441 gcaactggcg ctcaccactt tggggcatca cttcgtttac cgactgcgta gtgaatttat
     4501 caagcggatt ctggatactc acgtcgagcg cattgaacaa ctcggtagcg cctcgttgct
     4561 ggcgggatta accagcgatg tgcgcaatat caccattgct tttgtgcgtc tgccggaact
     4621 ggtgcagggg atcattctca ctatcggttc agcggcgtat ctgtggatgc tgtcgggcaa
     4681 aatgttgctg gtaacggcta tctggatggc agtcaccatc tggggcggtt ttgtgctggt
     4741 ggcgcgggtg tacaaacata tggcgaccct gcgtgaaacc gaagacaagc tgtacacgga
     4801 ttttcaaact gtacttgaag ggcgcaaaga gctgactctg aaccgggaac gcgccgagta
     4861 tgtgtttaac aacctctaca ttcctgatgc gcaagagtat cgccatcata ttattcgcgc
     4921 agacaccttc catcttagtg ccgtgaactg gtcaaacatc atgatgctgg gcgctatcgg
     4981 cctggtgttc tggatggcga acagtctcgg ttgggctgat accaacgttg ccgcgaccta
     5041 ttcgttgacg cttttattcc tgcgtacgcc gctgctttcg gcggttggcg cattgccgac
     5101 gctgctgacg gcgcaggtgg cgtttaacaa gctgaacaaa ttcgcgctcg cgcctttcaa
     5161 agcagagttt ccgcgcccgc aggcgtttcc cagctggcaa acgctggagc tgcgtaacgt
     5221 gacgtttgct tatcaggata acgcgttttc cgttggtccg attaatctca ccatcaaacg
     5281 tggcgagctg ctgtttctga ttggcggcaa cggtagcgga aaatcgacgc tggcgatgtt
     5341 gttgacgggc ttgtatcagc cacaaagcgg cgaaatcttg ctggatggca aacctgtcag
     5401 cggcgaacaa ccggaagatt atcgcaaact gttttcggca gtgtttaccg atgtctggct
     5461 gtttgatcaa ctgctggggc cggagggtaa acccgctaac ccgcaactgg ttgagaagtg
     5521 gctggcgcag ctgaaaatgg ctcataagct tgagttaaga aacgggcgta ttgttaacct
     5581 gaagttatca aaagggcaga aaaaacgcgt ggcgctgttg ctggcgctgg cagaagaacg
     5641 cgatattatc ctgctggatg aatgggcggc ggatcaggat ccacacttcc gtcgtgagtt
     5701 ttatcaggtg ttgctgccgc tgatgcagga gatgggtaaa actattttcg ccatcagtca
     5761 tgatgatcat tactttatcc acgccgaccg cctgctggaa atgcgcaacg ggcaacttag
     5821 cgagctgacg ggcgaagagc gcgatgccgc ttcgcgtgat gccgttgccc ggacggcata
     5881 atattacgac gctgacctgg cggactgctg ccgccaggtc aatatgctaa aaatcccttc
     5941 atttctaata accctataat taatcaacga aattataatg tttctaaaat tagaatataa
     6001 tttataaaca ttatttaaat gttgttactt aagtgttacc gttgatgccg cgcaaatctc
     6061 acctgcaata aagcgactaa aagtaaggca ttaacaagat gaaaaaagtg actgccatgc
     6121 tcttctcgat ggccgtgggg cttaatgccg tttcgatggc ggcaaaagcg aaagcgtccg
     6181 aggagcagga aactgatgta ctgttgattg gcggcggcat tatgagcgcc acgttgggga
     6241 cctatttacg cgagctggag cctgaatggt cgatgaccat ggtggagcgc ctggagggtg
     6301 tcgcgcagga gagttcgaac ggctggaata acgccggaac cgggcattct gcactgatgg
     6361 aactgaacta caccccgcaa aacgccgatg gcagcatcag tattgaaaaa gcagtcgcca
     6421 ttaacgaagc atttcagatt tcccgccagt tctgggcgca ccaggtcgag cgcggcgtgc
     6481 tgcgtactcc gcgttcattt atcaataccg ttccgcatat gagctttgtc tggggcgagg
     6541 ataacgtcaa tttcctgcgc gcccgttacg ccgcgttgca acaaagctcg ctgtttcgcg
     6601 gtatgcgtta ctctgaagat cacgcgcaga tcaaagagtg ggcaccgtta gtgatggaag
     6661 ggcgcgatcc gcaacagaaa gtggcagcca cgcgtacgga aattggtacc gatgtgaact
     6721 acggcgagat cacccgccag ttaattgctt ccttgcagaa gaaatctaac ttctcgctgc
     6781 aactcagcag cgaagtccgc gccctaaagc gtaatgacga taacacctgg accgttaccg
     6841 ttgccgatct gaaaaatggc actgcacaga acatccgtgc gaaatttgtc tttatcggcg
     6901 cgggcggtgc ggcactgaag ctgttacagg aatcggggat tccggaagcg aaagactacg
     6961 ccggtttccc ggtgggcgga cagttccttg tttcggaaaa cccggacgtg gttaatcacc
     7021 atctggcgaa ggtttacggt aaagcatccg ttggcgcacc accgatgtcg gttccgcata
     7081 tcgatacccg cgttctggac ggtaaacgcg tagtgctgtt tgggccattt gccaccttct
     7141 caaccaaatt cctcaaaaac ggttcattgt gggatctgat gagttccacc accacctcta
     7201 acgtgatgcc gatgatgcac gtcgggctgg ataatttcga tctggtgaaa tatctggtga
     7261 gtcaggtgat gttgagtgaa gaggatcgtt ttgaagcgtt gaaagagtac tatccgcaag
     7321 cgaaaaaaga ggactggcgt ttgtggcaag cggggcagcg tgtgcagatt atcaagcgtg
     7381 atgccgataa aggtggtgta ctgcgtctgg gtactgaagt ggtcagtgac cagcaaggaa
     7441 ccattgccgc gctcctgggg gcatcgccag gggcgtcaac cgccgcgccg attatgttga
     7501 atctgctgga aaaagtattt ggcgatcgcg tttccagccc gcaatggcag gctacgttga
     7561 aagcgatcgt tccatcttat ggacgcaagc tgaacggtga tgtagcggca acagaacgcg
     7621 agttgcagta caccagcgaa gtgctggggc tgaaatacga caaaccgcaa gcagcagata
     7681 gtacgccgaa accgcagttg aaaccgcaac ccgttcaaaa agaagtggcg gatattgcgt
     7741 tgtaatgatg tgccacatcc ggcatggtat gccggatgtg gcgtatgctg ataagacgcg
     7801 ccagcgtcgc atcaggcaac cggctcgggc gttagatgtc ggatgcggcg tgaccgcttt
     7861 atccaaccta cggggtgccg cacttcacgg cagtttagcg aactaccgcg ttgtcaattt
     7921 tctcttccgc cttccagacg cggtacttca catctacatt gtctggcgta tacaccacga
     7981 tcggcagctt gctgttgtaa cgcagcattc cagtatcgcc cagatacgcg gtgacaaatt
     8041 tcttctcttt cttgccatcc gggcaggcca tcatcgtgga aaccggcgag ctaactttat
     8101 caaagacata ataatcatag ccccagcctt ccagcgtttt gctttccagc ttcccgccga
     8161 gacgatgcaa attgcaatcg acttccagcg tctgaccgat taacagttct actttcaggg
     8221 tagattcatc ttcttgcggg gttaactgaa tcacctgacg cttcatgcct ttttcagctt
     8281 gtggataagg cgcgattttt tccagtggct ggacgctctc tgccgcccag gcggaagtgg
     8341 tggcgaaagc ggcaaacaat actgcaggta gaatggtctt cattttttgc tttccttctt
     8401 catttatagg tttgcaccga tgatgtcgct gaaggttaac atattcatca gatttattaa
     8461 tttgctttat gaataactta aagaatattc agacaatttt acctgctctt ttagtgttaa
     8521 attctgatga gagagcaaac cagcagaaat atctctggtg aattaat
//
LOCUS       DBFIFM010000184         8495 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.184, whole genome shotgun sequence.
ACCESSION   DBFIFM010000184 DBFIFM010000000
VERSION     DBFIFM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.184"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(83..289)
                     /locus_tag="N7C74_003802"
     CDS             complement(83..289)
                     /locus_tag="N7C74_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HGU2433595.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            602..691
                     /gene="kdpF"
                     /locus_tag="N7C74_003803"
     CDS             602..691
                     /gene="kdpF"
                     /locus_tag="N7C74_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HGU2433596.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALIHAEAF"
     gene            691..2364
                     /gene="kdpA"
                     /locus_tag="N7C74_003804"
     CDS             691..2364
                     /gene="kdpA"
                     /locus_tag="N7C74_003804"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HGU2433597.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLAWLINDIPLPGTTGVER
                     VLFSALGVSNHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALVIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2387..4435
                     /gene="kdpB"
                     /locus_tag="N7C74_003805"
     CDS             2387..4435
                     /gene="kdpB"
                     /locus_tag="N7C74_003805"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HGU2433598.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSVAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVVATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAVTYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4444..5016
                     /gene="kdpC"
                     /locus_tag="N7C74_003806"
     CDS             4444..5016
                     /gene="kdpC"
                     /locus_tag="N7C74_003806"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HGU2433599.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTDNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPNASTSVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5009..7693
                     /gene="kdpD"
                     /locus_tag="N7C74_003807"
     CDS             5009..7693
                     /gene="kdpD"
                     /locus_tag="N7C74_003807"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HGU2433600.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RKLALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGHWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7690..8367
                     /gene="kdpE"
                     /locus_tag="N7C74_003808"
     CDS             7690..8367
                     /gene="kdpE"
                     /locus_tag="N7C74_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HGU2433601.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPSHFITETG
                     IGYRFML"
BASE COUNT         1802 a         2201 c         2450 g         2042 t
ORIGIN      
        1 aatattcctc tgaacatctt cctgatacaa aaaaccgcca gcgtttccgc tggcggtttt
       61 caatgtattc aaaactcagc aatcagtaaa aatcaccggt tgccttttcg gcctgatcca
      121 tccaaaccgg tttatcgctg gttttcgacc agacgcgatg caggtagctg taaaagcggg
      181 cgcggtcttt ccagaaaagc atgaaaggca gcgccagaac gcccgctgca accgcataag
      241 tacggcgtaa aaagataagc catgcaggat attctctgta gagttccata cttttctccc
      301 ccttttgtat ctacccggtg aatagcaccg gaaaaatgaa tttgtttatc tgatgaaaat
      361 agtaccgcct tttgtgtaat tttactactc atccgaccac ttatttttgc ttattgatgg
      421 tttatttaca ttcatcctgt aattaagtta cacaaaagtt aaattaatac taaacattag
      481 ttaaatcatg gcttttgcca tttttatact ttttttacac cccgtccgca gatttttgcg
      541 aaatctttgc agccagaatt ctacccttcc ggtatcactt ttaggccact ggaggtgcac
      601 tgtgagtgca ggcgtgatag ccggcgtgtt gctggtgttt ttattactgg gttatttggt
      661 ttatgccctg atccatgcgg aggcattctg atggctgcgc aagggttctt actgattgcc
      721 acgtttttac tggtattaat ggtgctggcg cgtcccttag gtagcgggct agcgtggctg
      781 attaatgata ttcctcttcc tggtacgacg ggcgttgaac gcgtactctt tagcgcgctt
      841 ggcgtctcta accatgagat gaactggaag cagtatctct gtgccattct cggcctgaac
      901 atgctggggc tggcggtgct gttttttatg ttgctcggtc agcactatct gccgcttaat
      961 ccacagcagt tgccggggct gtcgtgggat ctggcgctga ataccgccgt cagctttgtc
     1021 acgaatacca actggcaatc ttatagcggt gaaaccacgt tgagctattt cagccagatg
     1081 gcgggcttaa cggtgcaaaa ctttctttct gccgccagcg ggattgcggt gatttttgcc
     1141 ctcattcgtg catttacccg ccagagcatg agcacactcg ggaatgcctg ggtcgatctg
     1201 ctacgcatca cgttatgggt gctcgtccct gtggcgttgg tgatcgcact gttttttatt
     1261 caacaaggtg cgctgcaaaa ctttctgccc tatcaggcag tgaataccgt tgaaggagcg
     1321 caacagctgt taccaatggg tccggtggct tctcaggaag cgatcaagat gctcggcact
     1381 aacggcggtg gtttctttaa tgccaactcg tcgcatccgt ttgaaaaccc aaccgcactg
     1441 accaacttcg tgcagatgct ggcgatcttc ttgatcccaa cagcgctgtg ctttgccttt
     1501 ggtgaagtga cgggcgatcg ccgtcagggg cgcatgttgc tgtgggcgat gtcagtgatt
     1561 tttgtcatct gcgtaggcgt ggtgatgtgg gcagaagttc agggtaatcc tcatctgctg
     1621 gcgctcggcg cagacagcag catcaatatg gaaggtaaag agagccgttt cggcgtgctg
     1681 gtcagtagcc tgtttgcggt cgtgactacg gcggcgtcct gtggcgcggt gattgcaatg
     1741 catgattcgt ttaccgctct cggtggcatg gtgccgatgt ggctgatgca aattggtgaa
     1801 gtggtattcg gcggtgtcgg ttctggtctt tacggcatga tgctgtttgt cctgctggcg
     1861 gtgtttattg ccgggctgat gattggtcgt acaccggaat atctgggtaa aaaaatcgac
     1921 gtacgtgaga tgaaactgac tgcactggcg attctggtca ccccgacgct ggtgctgatg
     1981 ggcgcggcgc tggcaatgat gaccgacgcc ggacgtagcg ccatgctcaa ccctggcccg
     2041 catggtttta gcgaagtgct gtacgccgtg tcatccgccg ctaacaacaa cggcagcgcc
     2101 tttgccggat taagcgccaa ctctccgttc tggaactgtt tactggcgtt ctgcatgttt
     2161 gtcggtcgct tcggggtgat tatcccggtg atggcaattg ccggttcgct ggtgagtaaa
     2221 aagagccaac ccgccagctc cggcacgctg ccaacgcacg gcccgctgtt tgttggcctg
     2281 ttaatcggca ccgtgttgct ggttggcgca ctgaccttta tccctgccct ggcgcttggt
     2341 ccggtggcgg aatatctctc ctgatgatat tgagtgagca ctgaatatga gtcgtaaaca
     2401 actggcgcta ttcgaaccaa cacttgtcgt tcaggcgctg aaagaagcgg tgaaaaaatt
     2461 aaacccgcag gcgcaatggc gcaatccggt gatgtttatc gtctggatcg gcagtctgct
     2521 gaccacctgt attagcatcg cgatggcaag cggcgcgatg cctggcaatg cgctgtttag
     2581 cgtggccatt agcggctggc tgtgggtcac cgtactgttc gctaatttcg ccgaggcgct
     2641 ggcagaaggc cgcagtaaag cgcaggccaa cagcctgaaa ggggtgaaaa aaacggcctt
     2701 tgcccgcaag ctgcgtgagc cgaaatatgg cgctgcggcg gacaaagttc ccgccgatca
     2761 actgcgtaaa ggcgatatcg tactggtaga agcaggcgat attatcccct gcgatggtga
     2821 agttattgaa ggcggcgcat cggtcgatga aagcgccatc accggggaat cggcaccggt
     2881 gatccgtgaa tccggcggcg attttgcctc cgtcaccggc ggcacgcgta ttctttctga
     2941 ctggctggtg attgagtgta gcgttaaccc cggcgagaca tttctggatc ggatgatcgc
     3001 gatggtggaa ggcgcacagc gacgcaaaac gccgaacgag attgccctga ccatcctgct
     3061 gattgccctg actatcgtct ttttactggc aaccgccacg ctgtggccgt tttccgcgtg
     3121 gggcggtaat gcagtcagcg taacggtact ggtggcgctg ctggtctgtc tgatcccaac
     3181 cactattggc ggcctgttgt cagcgatcgg cgtcgccggg atgagtcgga tgctgggcgc
     3241 gaatgtggtt gccaccagcg gacgtgcagt tgaagcggca ggtgacgttg acgttctgct
     3301 gctggataaa accggcacca tcacactcgg taaccgtcag gcatcggagt ttatccctgc
     3361 gcagggcgtg gatgaaaaaa cgctggctga cgccgcacaa ctggcttcgc tggctgatga
     3421 aacgccggaa ggccgcagta ttgtgatcct cgccaagcag cgttttaacc tgcgcgagcg
     3481 cgatgtgcag tcgctccatg ccacctttgt accgtttact gcgcaaagcc ggatgagcgg
     3541 gatcaatatc gacaaccgca tgatccgtaa aggttctgtc gatgccattc gccgccatgt
     3601 tgaggctaac ggtggtcact tccctgccga tgttgatcaa aaagtcgatc aggttgcgcg
     3661 tcagggggcc acaccgctgg tagtagtaga aggttctcgt gtgctgggcg ttattgcgtt
     3721 gaaagatatc gtcaaaggcg gcattaaaga acgcttcgcc caactgcgca aaatgggtat
     3781 taaaacggtg atgattaccg gcgataaccg tctgactgcc gccgcgatag ctgcggaagc
     3841 gggcgtcgat gattttctcg ccgaagcgac accggaggcc aagctggcat tgatccgtca
     3901 gtatcaggcg gaaggccgtt tggtagcaat gaccggcgac ggcaccaacg atgctccggc
     3961 gctggcgcag gcagatgtcg cggtggcgat gaactccggt actcaggcgg cgaaagaagc
     4021 gggcaatatg gtggatctcg actctaaccc gaccaagttg atcgaggtgg tacatattgg
     4081 caaacagatg ctgatgaccc gtggctcgct gaccaccttc agcattgcca acgatgtggc
     4141 gaaatacttc gccattattc cggcggcatt cgcggtaacg tatccacagt taaatgcgct
     4201 gaacatcatg cgcctgcatt cgcccgactc cgccattctc agtgcggtca ttttcaacgc
     4261 cttgattatc gtctttttga ttcccctggc gttaaaaggc gtgagttata aaccgcttac
     4321 cgcttctgcc atgttgcgcc gtaacttatg gatttacggt ctgggtgggc tgctggtgcc
     4381 gtttattggt atcaaagtca ttgatttact actgaccgtt tgcggtctgg tgtgaggttt
     4441 accatgcgtg gattacgtcc ggcattatcg acatttatct ttctgttatt gattactggc
     4501 ggtgtttacc cgctgctgac caccgcgctg gggcaatggt ggtttccctg gcaggccaac
     4561 ggttcgttga tccgtgaagg tgatacggtg cggggttcgg cattaatcgg gcagaatttt
     4621 accgacaacg gctattttca tggtcgcccg tcggcaacgg cagaaatgcc ttataatcca
     4681 caggcttctg gcgggagcaa tctggcggtc agtaaccctg agctggataa actaatagcc
     4741 gcacgcgttg ctgcattacg ggccgctaac ccgaatgcca gcacgagcgt tccggttgaa
     4801 ctggtgacgg cgtcggcaag cgggctggac aataacatca ccccgcaagc ggcggcctgg
     4861 cagatcccac gcgtagcgaa agcgcgtaat ctcagcgttg aacagctcac gcaactgatc
     4921 gcaaaataca gccaacaacc actggtgaaa tatatcggcc agccggtcgt caacattgtc
     4981 gaactcaatc tggcactgga taaacttgat gaataacgaa cccttacgtc ccgaccccga
     5041 tcgtctgctg gaacaaactg ccgcgccgca tcgggggaag ctgaaagttt tcttcggtgc
     5101 ctgcgcaggc gtcgggaaga cctgggcgat gctggcagaa gcccagcgac tgcgggcgca
     5161 agggctggat attgtggttg gcgtggtaga aacccacggg cggaaagata ccgccgccat
     5221 gctggaaggg ctggctgttc tgccgccaaa acgcctggcg taccgtgggc ggcacatcag
     5281 cgagtttgat ctcgatgccg ccctcgcccg ccgtccggcg ctgatcttaa tggacgaact
     5341 ggcgcacagt aatgcgccag gttctcgtca tcctaaacgc tggcaggata tcgaagaact
     5401 gctggaagct ggcattgatg ttttcacaac cgtcaacgtt cagcatctgg aaagtctgaa
     5461 tgatgtggtc agcggcgtca ccggaattca ggtacgggaa accgtgcccg atcctttttt
     5521 cgatgccgcc gacgacgtgg tgctggtgga cttgcccccg gacgatctgc gccagcggct
     5581 gaaagaaggc aaagtctata ttgccgggca ggcggagcgc gccattgaac attttttccg
     5641 caaaggcaat ctgatcgccc tgcgcaaact ggcactgcgc cgtactgccg atcgcgttga
     5701 tgagcaaatg cgcgcctggc gggggcatcc tggcgaagag aaagtctggc acacgcgcga
     5761 cgcgatcctt ttatgcatcg gccataacac cggcagcgaa aaactggtcc gcgcggcggc
     5821 gcggctggcg tcacggctgg ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct
     5881 gcatcgctta ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga
     5941 gctgggcgcg gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta
     6001 tgcccgtgaa cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg
     6061 gcgtcgggaa acgtttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct
     6121 ggtcgcgctt gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcttt
     6181 taaagacaag tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt
     6241 tatcacctta attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct
     6301 gtatctactt ggcgtggtgg tggtggcgct attttatgga cactggcctt cggtggttgc
     6361 taccgtcatt aatgtggtga gtttcgatct cttttttatc gccccacgcg gcacgctcgc
     6421 cgtctctgat gtgcaatatc tgctgacctt cgcggtgatg ttaaccgtcg ggctggtgat
     6481 cgggaacctt actgcgggtg tgcgttatca ggcgcgggta gcccgttacc gcgagcaacg
     6541 cacacggcac ttatatgaaa tgtcgaaagc tctggcggtg ggccgcagtc cgcaggacat
     6601 cgccgccacc agcgaacaat ttattgcctc cacgtttcat gcccgcagtc aggtgttgtt
     6661 gcccgatgac aacggtaaat tgcagccgtt aacacatccg caaggaatga cgccgtggga
     6721 cgatgccatc gcgcagtgga gttttgataa aggcctgcct gcgggtgcgg gcaccgacac
     6781 gttacccggc gtgccgtacc agattttgcc gttaaaaagc ggtgagaaaa cctacgggct
     6841 ggtggtggtg gaaccgggga atctgcgtca gttgatgatc ccggaacagc agcgcctgct
     6901 ggagacgttt acgctgttag tcgccaatgc gcttgagcgg ctgacgctga ccgccagcga
     6961 agaacaggcg cggatggcaa gcgaacgtga acagatccgc aacgccctgc tggcggcgct
     7021 ttcgcatgat ttacgcactc cgcttacggt gctgtttggt caggcagaaa tcttaacgct
     7081 cgatctggca agcgaaggat caccccacgc ccgccaggcc agcgagattc gtcagcatgt
     7141 gctgaacact acccgactgg tgaataatct actggatatg gcgcgaattc agtccggcgg
     7201 ctttaatttg aagaaagagt ggttaacgct ggaagaagta gtcggcagcg cgctgcaaat
     7261 gctggaaccg ggtttatcgt cgcccatcaa tctttctctg ccagaaccgc tgaccttaat
     7321 ccacgttgac gggccactct ttgaacgggt gctgattaat ctgctggaga acgcggtgaa
     7381 atatgcgggt gcgcaggccg aaattggtat cgatgcccac gttgagggcg aaaatctaca
     7441 actggatgtc tgggataacg gccccggtct tccgccaggc caggagcaga cgatatttga
     7501 taagtttgct cgcgggaata aagagtcggc agtaccgggg gtagggcttg gactggcaat
     7561 ttgtcgggcg atagtggatg tacacggggg cactattacc gcgttcaacc ggccggaagg
     7621 tggtgcctgt tttcgtgtta cacttcccca gcaaactgcc cctgaacttg aagaatttca
     7681 tgaggatatg tgacaaacgt tctgattgtt gaagatgaac aggctattcg tcgctttctg
     7741 cgcacggcgc tggagggcga cgggatgcgc gtctatgagg ccgaaacgct gcaacgcggc
     7801 ttgctggaag cggctacccg taagccagat ttgattattc tcgatctcgg cctgcccgat
     7861 ggtgatggga ttgagtttat ccgcgacctg cgccagtgga gcgcggtgcc ggtgattgtg
     7921 ctttccgcac gcagcgaaga gagcgacaaa atcgccgcgc tggatgccgg agcggatgat
     7981 tatctaagta agccgtttgg cattggcgaa ttgcaggccc gtctgcgcgt cgctttacgc
     8041 cgccactccg ccaccgccgc gcccgatccg ctggtgaaat tttccgatgt taccgtcgat
     8101 ttagccgccc gcgtgattca ccggggtgag gaagaggtgc atctcacccc gattgagttt
     8161 cgcctgctgg cagtgctgct caacaacgcc ggaaaagtgc tcacccagcg ccaactcctt
     8221 aaccaggtgt gggggccaaa cgcggtcgaa cacagtcatt atttgcgtat ttatatggga
     8281 catctgcgac aaaaactgga acaggatccc gctcgcccca gccatttcat tactgaaacc
     8341 ggtattggtt atcggtttat gctttgaatt tttattcaat aaattcgcct aaatagtgat
     8401 taatttatta ttaaacctgc gccaaatatt attatttggc aggtttaatt tcttaacgca
     8461 aatataaaac acgaaattac aacatttaag taaca
//
LOCUS       DBFIFM010000185         8490 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.185, whole genome shotgun sequence.
ACCESSION   DBFIFM010000185 DBFIFM010000000
VERSION     DBFIFM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.185"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(151..227)
                     /locus_tag="N7C74_003809"
     tRNA            complement(151..227)
                     /locus_tag="N7C74_003809"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(191..193),aa:Asp,seq:gtc)
     gene            complement(360..1091)
                     /gene="dnaQ"
                     /locus_tag="N7C74_003810"
     CDS             complement(360..1091)
                     /gene="dnaQ"
                     /locus_tag="N7C74_003810"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HGU2433602.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRLVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1156..1623
                     /gene="rnhA"
                     /locus_tag="N7C74_003811"
     CDS             1156..1623
                     /gene="rnhA"
                     /locus_tag="N7C74_003811"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HGU2433603.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1620..2342)
                     /locus_tag="N7C74_003812"
     CDS             complement(1620..2342)
                     /locus_tag="N7C74_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU2433604.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFY
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2376..3131
                     /gene="gloB"
                     /locus_tag="N7C74_003813"
     CDS             2376..3131
                     /gene="gloB"
                     /locus_tag="N7C74_003813"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HGU2433605.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3203..4561
                     /gene="mltD"
                     /locus_tag="N7C74_003814"
     CDS             3203..4561
                     /gene="mltD"
                     /locus_tag="N7C74_003814"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HGU2433606.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNSMP
                     DS"
     gene            complement(4609..5379)
                     /locus_tag="N7C74_003815"
     CDS             complement(4609..5379)
                     /locus_tag="N7C74_003815"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU2433607.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQLLAVCLADYP
                     DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAQMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALCDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVDAHKAA"
     gene            complement(5457..6257)
                     /locus_tag="N7C74_003816"
     CDS             complement(5457..6257)
                     /locus_tag="N7C74_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGU2433608.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6498..7412
                     /gene="yafC"
                     /locus_tag="N7C74_003817"
     CDS             6498..7412
                     /gene="yafC"
                     /locus_tag="N7C74_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HGU2433609.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPVACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7409..8212)
                     /gene="dkgB"
                     /locus_tag="N7C74_003818"
     CDS             complement(7409..8212)
                     /gene="dkgB"
                     /locus_tag="N7C74_003818"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HGU2433610.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGLAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKEGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(8375..8451)
                     /locus_tag="N7C74_003819"
     tRNA            complement(8375..8451)
                     /locus_tag="N7C74_003819"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8415..8417),aa:Asp,seq:gtc)
BASE COUNT         2259 a         2036 c         2073 g         2122 t
ORIGIN      
        1 tgaattttaa tgaatgagac aacaagcgag aatcacgcac ttacgccaga gtaaaatcat
       61 tgaacagaga aatggtggaa ttcaaataca aaaaaaaccg caaaattaaa aatcttgcgg
      121 ttctctgaac tcattttcat gaggtgaatt tggcggaacg gacgggactc gaacccgcga
      181 ccccctgcgt gacaggcagg tattctaacc gactgaacta ccgctccgcg ttgtgttccg
      241 ttgggaacga ggcgaatatt acggattgcc tcacccttcg tcaacggttt ttctcatctt
      301 ttgaatcatt tgctgcaaaa atcgcccaag tcgctatttt tagcgccttt tacatgtatt
      361 tatgcccgcc agaggcagct tccgcccttt ttctgcacca gatcgagacg ggcttcatgt
      421 gctgcaagct cttcatctgt cgcaaaaaca acgcgtaact tacttgcctg acgtacaagg
      481 cgctggattg ttgcttcacc ttgttgctgt tgtgtctctc cttccatcgc aaaagccatc
      541 gacgtttgac caccggtcat cgccagataa acttccgcaa ggatctgggc atcgagtaat
      601 gccccgtgca gcgttcgttt actgttatct atttcgtagc gggcacataa cgcatcgagg
      661 ctgttgcgtt taccgggaaa cattttcctc gccaccgcaa ggctatcggt gaccttacag
      721 aaggtattag tcttcggaat atcgcgctta agcaacgaga actcgtagtc cataaagccg
      781 atatcgaacg ctgcgttatg gatcaccaac tccgcgccgc ggatatagtc catgaactca
      841 tcggctactt cggcaaacgt gggcttatcg agcaaaaatt catcggcaat accatgtacg
      901 ccaaaggctt ccggatccac cagccgatcg ggtttgagat aaacatggaa gttattgccc
      961 gtcaggcgac ggttcaccac ttcaacggca ccaatctcaa tgatcttgtg gccttcatag
     1021 tgcgcaccaa tctggttcat accggtggtt tcggtatcga gaacgatctg gcgtgtaatt
     1081 gcagtgctca tagcggtcat ttatgtcaga cttgtcgttt tacagttcga ttcaattaca
     1141 ggaagtctac cagagatgct taaacaggta gaaattttca ccgatggttc gtgtctgggt
     1201 aatcctggac ctgggggtta cggcgctatt ttacgctatc gcggacgcga gaaaaccttt
     1261 agcgcaggct acacccgcac caccaacaac cgtatggagt tgatggccgc tattgtcgcg
     1321 ctggaggcgt taaaagaaca ttgcgaagtc attttgagta ccgacagcca gtatgtccgc
     1381 cagggtatca cccagtggat ccataactgg aaaaaacgtg gctggaaaac cgcagacaaa
     1441 aaaccagtaa aaaatgtcga tctctggcaa cgtcttgatg ctgcattggg gcagcatcaa
     1501 atcaaatggg aatgggttaa aggccatgcc ggacacccgg aaaacgaacg ctgtgatgaa
     1561 ctggctcgtg ccgcggcgat gaatcccaca ctggaagata cgggctacca agtcgaagtt
     1621 taagcctgtg gtttacgaca ttgccgggtg gctccaaccg cctggcgaat tcgtggcttg
     1681 tttttactct gtttcatcgg atttagcgtt aaaggaatag tccgtttccg ggcaacaata
     1741 agttgtaagc aaccaagcgc aggaatatgc gcattcaaaa gttttcctcc gtgtttgttc
     1801 cacgggagaa cgtggaaacg gctggcgtgt agcacttcaa aattcaacaa agagagccag
     1861 tccagttgcc gcatcagagt aaacatccgg ctgttatagg gcgaggtttt gcgcaatacc
     1921 ggcacaagtt tgcgtaatcc cattaaacta atgggattga agccactaat gaccagccag
     1981 ccatcatcaa tcaatacccg atcggcttca cgcaataaac gatgcggatc ggtgcaccac
     2041 ggcaatgtat gtgccagtag acaaacatca acggatttat cggcaaaagg aagatgaagt
     2101 gggtccgcct gcacctggac aggcattcct tgcgcagaaa cattcacttg atgagaaacc
     2161 gcgcacgctt cgcaattgat ttctgcgctt aaattgccaa tcttaagcag ataaaaacca
     2221 tacattttag tgaaccacgg gttgagctgg cgctccagcg ccttgcgata aagctctccc
     2281 cagggcaaat cgccccagcg atcaggagcc acgacagttt gagggactct tgccggtttc
     2341 atcacaacct tccgtttcac actgagaggt aatctatgaa tcttaacagt attcccgcct
     2401 ttgatgacaa ttacatctgg gttttgaatg atgaagcagg tcgctgcctg attgtcgatc
     2461 ccggagacgc agagccagta ttaaacgcca ttgccgccaa taactggcaa ccggaggcca
     2521 tatttctcac ccaccatcat cacgatcacg ttggcggcgt aaaagaactg gtggaaaagt
     2581 ttccgcaaat tgtggtgtat ggtccacaag agacacaaga taagggaaca acacaggtag
     2641 tcaaagatgg cgaaactgcc ttcgttttgg ggcatgaatt tagtgtaatt gccacgccgg
     2701 gtcacacttt aggacatatc tgttacttca gtaaacctta tctattttgc ggcgacacgc
     2761 tgttttctgg tgggtgtggt cggttgtttg aagggacagc atcacaaatg tatcaatcac
     2821 ttaaaaagtt aagtgcgtta cctgacgata cattggtatg ttgtgctcat gaatatacgt
     2881 tatcaaatat gaagtttgct ttgagtattc ttccgcacga tttgtccata aatgattatt
     2941 atcgtaaagt taaggagtta cgggcaaaaa atcaaataac actacccgta attctgaaaa
     3001 atgagcggca aattaatgtt tttttaagaa cggaagatat tgatttaatt aatgtaatta
     3061 atgaagaaac attattgcaa caacctgaag agcgttttgc atggttaagg tcaaagaaag
     3121 ataggttctg ataaaacttt cttgtcatcg gctccgttcg ccgttatgat cggtcgtctt
     3181 ttaagcaact attgacgcac acatgaaggc aaaagcgata ttactcgcct ctgtcctgct
     3241 cgtgggttgc cagagtaccg gcaacgttca acagcacgca cagagccttt ctgcagctgg
     3301 tcaaggggaa gcagcaaagt ttacaagtca ggcacgatgg atggacgatg ggacgtctat
     3361 cgcgccagat ggtgacttgt gggctttcat tggcgacgag ctaaagatgg gaattccgga
     3421 aaatgaccgg attcgcgaac agaaacagaa atatttacgc aataagagct atctccacga
     3481 tgtaacttta cgggcagagc cgtatatgta ctggatagcc gggcaagtta aaaaacgtaa
     3541 catgcctatg gaactggtac tactacccat agtggagagc gcttttgatc ctcacgcaac
     3601 gtctggcgcc aatgccgcag gcatctggca gatcattccg agcacggggc gcaattatgg
     3661 tttgaaacag acccgcaatt atgacgcgcg tcgcgatgtt gttgcttcaa caactgccgc
     3721 gctgaatatg atgcagcgtc tgaacaaaat gtttgatggc gactggcttc tgaccgtagc
     3781 ggcttataac agcggcgaag gtcgggtcat gaaggcaatt aaaacgaaca aagcgcgtgg
     3841 gaaatccacg gacttctggt cgttaccgtt gccgcaggaa acgaagcagt acgtgcctaa
     3901 aatgctggca ttgagtgata ttctcaaaaa cagcaagcgt tatggcgtac gtctgccaac
     3961 gaccgatgaa agccgtgctc tggcgcgtgt gcacctgagt agcccggttg aaatggcgaa
     4021 ggttgcagat atggcgggga tttccgtcag caagctgaag acattcaacg ctggcgtgaa
     4081 aggctccacg ctgggcgcaa gtggtccgca gtacgtgatg gtgccaaaga agcatgcaga
     4141 tcaactgcgt gaatctctgg cttcaggcga aattgctgct gtacagtcga cgctggttgc
     4201 cgacaatacg ccgcttaaca gccgtgttta caccgtacgc tctggcgaca cgctttcaag
     4261 tatcgcttca cgtctcggcg taagcaccaa agatttgcag cagtggaaca aactgcgcgg
     4321 ctctaagctg aaaccaggcc aaagtctgac gattggcgca ggtagtagcg cacagcggtt
     4381 ggcaaacaac agcgatagca ttacgtatcg tgtgcgcaaa ggcgattcgc tttcaagcat
     4441 tgctaaacgc cacggcgtga acatcaaaga tgtgatgcgc tggaacagcg atactgcgaa
     4501 tctgcaacca ggcgataagc tgacgttgtt tgtgaaaaac aacagcatgc cagactcctg
     4561 acaaaccaga taatagaaag gcaccgattc ccccggtgcc ttttttattt atgccgcttt
     4621 atgtgcatct accatgatga tatcactggt gaaagagcca tcattctgca aggcaaaata
     4681 cgttctcacc tctgtcgatg cgctctgttg gtaaatgcga atagcgtcta ctaacgcttc
     4741 tggcgtacgc attctcgcga cccatgaaga aaattccagc ggtaacttat ctgtaattaa
     4801 attatcaact atcagattgg cttcattgat taacctcaac cactcaccgc tggcgtagtt
     4861 tcgtacgtga gaggtatcgc ataatgcttc taccgtctgt aaccagatgt cgcgcactgg
     4921 gtggcccgga gacattacgt ccatcacaat cagcctaccg ccaggtttca atatccggtt
     4981 cacttcacgc agtgctgcac caacatcatg ccagtgatgg gcagaataac ggctgataac
     5041 aatatcaaat gcgttatcgg caaatggcag actttcggca tacccctggc gggtggtgat
     5101 atttttcagt tgccggactt cggcagcttg tgccacgaca tccagcattt gggcagataa
     5161 gtcatacgcc accaccgtgc tcacattttg cgcagcgaca aagctggcat gccctgctcc
     5221 gcagcccata tcaagtacac ttgcatcagg ataatcagcc agacacaccg ccaggagctg
     5281 taaatctcgt ccggatgcat gtacggtact ggtcagatat tcgctggctt gagagctaaa
     5341 ctgcttttct acgtggtcat ggtgggattg tgttgtcatt gcactgtcct tttgttgttt
     5401 gagtataaaa gggcagctcc aaacgttggt ctgccctgtg gcagacctga cataccttat
     5461 ttatcaggct tgccgggact gaattcaacg agtagcggat tgtgatcgga cgcgcgcgta
     5521 accagtacag aagcttcgct gacgttcaga ccacggtaga aaacaaaatc gagcgggcga
     5581 ccaaacgccc gacggcgctg atcatcggta aaacgcacct gacgcagcga catttcccgc
     5641 gcaaaacgat ataacgcgtt catccttcta cggctccaag cattgaaatc tcccgccata
     5701 atgaccgggc cgctgtggtg agctatctga tcaccaatag gaagtaactg cttactataa
     5761 acatccacgc ccagactgaa gttgacggcg tgtatattaa ccaccattaa caggcgggtg
     5821 tcaggtaatg gatagaccgt caccagtgcc gacttcgcca gacgcaaaat gggttctcgt
     5881 tcacgtaacg ggcagcaata cactggatgt gccgccgaaa gggtcattac gccagaagga
     5941 tgttgtggca gcacgaaagc gggtacctga tcggcggcaa gatagttagc agtcgcaaac
     6001 tgtaccaact ctggcgttgt ttgcgcttcc tgcaataaca ccagatgtgc gtctttgccg
     6061 taattcttta ataccgacaa ccattcagcg cgttgctgtt tgtatatgtt ccacaccagg
     6121 atccgaatac gctcttcggt acttaacggt tctccgggtg gtaatgcctg gccgatgctc
     6181 gcaaaagacc ccggcggtaa gatcctctcc gcaggttgtc cggcaacata gcgcatggca
     6241 taggtgtttt ttcgcacttt tgactttgaa acctctatta cgtcaaccct tccgcactcg
     6301 ggagggttct actgttatag ggattttagc tcactctttc aacgagcaat tgtatattgt
     6361 tatgtaagca agtgcttaca ccggtcagaa ttttgctcat tattgagaac attaaatcgg
     6421 atgacatatc acgcatcctg atggtttaat gacaaataaa ttcaaaatat ctcatctatt
     6481 ttgcaacagg agcaaaaatg aaagccacgt cggaagaact cgccattttt gtttcggtcg
     6541 tcgaaagcgg cagctttagc cgggcagcgg aacaattagg gcaagcaaac tcagcggtaa
     6601 gccgggcggt gaaaaagctg gagatgaaac ttggcgttag cctgcttaat cggaccacgc
     6661 ggcaacttag cctgacggaa gaaggcgagc gttattttcg tcgcgtacag tcaattttgc
     6721 aggagatggc agcggcagaa tcagaaatta tggagacgcg taatacaccg cgtggactgt
     6781 tgcgcatcga tgccgcaact ccagtggtgc tgcactttct gatgccgttg attaagcctt
     6841 ttcgtgaacg ctatccggaa gtcactttat cgctagtctc ctccgaaacg attattaatt
     6901 tgatcgaaag aaaagtggat gtcgcgatac gcgccggtac gttaacggat tccagcttac
     6961 gtgccaggcc gttatttaac agttatcgaa aaattatcgc ctcccccgat tatatttccc
     7021 gctacgggaa gccagaaacg atcgacgatt taaagcaaca tgtttgcctg ggattcactg
     7081 aacccgcttc cctcaatacc tggccggtag cctgtagcga tggacaatta catgaggtga
     7141 agtacggttt gtcatccaat agtggggaaa cactgaaaca gctttgcctg agtggcaacg
     7201 ggattgcgtg tttgtccgac tatatgatcg acagagaaat cgctcgcgga gaattggtgg
     7261 agttaatggc agataaagtg ttgccagtgg aaatgccatt cagtgccgtc tattacagcg
     7321 accgtgcggt aagtacgcgc atccgggctt ttatcgattt ccttagcgag catgtaaaaa
     7381 cagctcccgg aggagctgtc agagaggctt aatcccattc aggagccaga ccttccgggc
     7441 taaccaggcg gtcgttgcaa tccagtgcgg cgatcgcttt tttatcttcg gcatcaagct
     7501 gtaaattttg tgccttaaga ttactctcca ggttttcacg tttagtagaa gaaggaatta
     7561 ctgagtaacc ttcccccata gcccacgcca gaatcacttg tgccggagtc gcattgtgtt
     7621 tagctgcgat acgagcaata acctcatctt tcagggcctt accatacgcc agcgtcatat
     7681 aggaagtaat atggatgccg tgctgtttag cccaggcaac cactttacgg ttttgcagat
     7741 aaggagagag ttcaatctgg ttagtagcga tgttttcagc gccaacagca gcaatcgcct
     7801 tttccatcaa tgggatcgtg aagttggaaa taccgatctc acgcgtcaac ccttcttttt
     7861 tggcttccag cagcgcctgc ataaactctt caacagagac ttcatcgttt ggtgacggcc
     7921 agtggattag cgtcagatca acataatcgg tacgcaattt ttgcaggctc tcttttagac
     7981 tcgggatcaa tttgtctttg ctgagatttt caatccagat tttagtggtg atgtagagtt
     8041 catgacgtgg cacgccactt tctgcaatcg ccagacctac tgcggcttcg ttatcataga
     8101 tttgtgcagt atcaattgcg cgataaccaa gttcaagcgc cgttttcaca gatgaaataa
     8161 caacgtcgtc tttcagacgg aaagtaccta aaccaaatgc agggatagcc ataatatgcc
     8221 tcttttaatg ctattttcgt taactgaggt gattatgggc tttagaatgt tgcggaaaaa
     8281 gggcgtaaaa cgcagaggat ttttgcgatt ttagcaataa tagaaatgta gacgaaaaaa
     8341 aacctcgagc gataactcga ggcttcttaa taagtggcgg aacggacggg actcgaaccc
     8401 gcgaccccct gcgtgacagg caggtattct aaccgactga actaccgctc caccgaattc
     8461 ttttacaacc accggtttta tgaccggctt
//
LOCUS       DBFIFM010000186         8453 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.186, whole genome shotgun sequence.
ACCESSION   DBFIFM010000186 DBFIFM010000000
VERSION     DBFIFM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.186"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            212..640
                     /gene="uspC"
                     /locus_tag="N7C74_003820"
     CDS             212..640
                     /gene="uspC"
                     /locus_tag="N7C74_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HGU2433611.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(647..2071)
                     /gene="otsA"
                     /locus_tag="N7C74_003821"
     CDS             complement(647..2071)
                     /gene="otsA"
                     /locus_tag="N7C74_003821"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HGU2433612.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLV"
     gene            complement(2046..2846)
                     /gene="otsB"
                     /locus_tag="N7C74_003822"
     CDS             complement(2046..2846)
                     /gene="otsB"
                     /locus_tag="N7C74_003822"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HGU2433613.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDINVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(3013..3999)
                     /gene="araH"
                     /locus_tag="N7C74_003823"
     CDS             complement(3013..3999)
                     /gene="araH"
                     /locus_tag="N7C74_003823"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HGU2433614.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVA
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(4014..5528)
                     /gene="araG"
                     /locus_tag="N7C74_003824"
     CDS             complement(4014..5528)
                     /gene="araG"
                     /locus_tag="N7C74_003824"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HGU2433615.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(5598..6587)
                     /gene="araF"
                     /locus_tag="N7C74_003825"
     CDS             complement(5598..6587)
                     /gene="araF"
                     /locus_tag="N7C74_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HGU2433616.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            7384..7887
                     /gene="ftnB"
                     /locus_tag="N7C74_003826"
     CDS             7384..7887
                     /gene="ftnB"
                     /locus_tag="N7C74_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HGU2433617.1"
                     /translation="MATAGMLLKLNSQLNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(7966..8217)
                     /gene="yecJ"
                     /locus_tag="N7C74_003827"
     CDS             complement(7966..8217)
                     /gene="yecJ"
                     /locus_tag="N7C74_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HGU2433618.1"
                     /translation="MSQTLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8332..8418)
                     /gene="azuC"
                     /locus_tag="N7C74_003828"
     CDS             complement(8332..8418)
                     /gene="azuC"
                     /locus_tag="N7C74_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HGU2433619.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
BASE COUNT         2201 a         2136 c         1932 g         2184 t
ORIGIN      
        1 tatattaacc acttgtttaa tgcggggaat cagtacatta ttttcttata attacagttg
       61 aaatcatatg atcaccgaga gtgattgatt aattgtcttt actaataatc ggactaatat
      121 ttacctgttt gactgagttg ggattgcgtc gtttctccat taggagtaaa gctttaatgt
      181 cacctgaagt tcacagaata aggaacaggt tatgagctat agcaatattc ttgtcgctgt
      241 tgcggttaca ccggaaagtc agcaactgct ggcaaaagcg gtatctatcg ccaggccagt
      301 aaagggacac atcagtttaa ttactctcgc ttccgacccg gaaatgtaca atcaattagc
      361 tgcgccgatg cttgaagatt tacgcagcgt gatgcaggaa gaaacgcaaa gctttcttga
      421 taagttaatt caggatgcgg ggtatcccgt tgacaagacg tttattgcct acggtgagtt
      481 aagcgaacat attctggaag tttgtcgcaa atatcatttc gatttggtga tttgcggtaa
      541 tcacaatcac agtttctttt cgcgagcatc ctgctcggcg aaaagagtga ttgcctcaag
      601 tgaggtcgat gtgctgttag ttccacttac gggagattaa ccgctcctac acaagcttag
      661 gaaaggtagc aactttatcg cgctgctggc tttccgcgct tcgcggaact atctgcttta
      721 ggtcgctaat gaagcactcc tgccagtggt taatatcgtt tttcacgata acgtccagca
      781 tttctgcatg acgggaaata cgttccgcca gcgacatagt caatgcacga tccagcgcag
      841 ctgcaacttc gtcacgatcg tagggattaa caattaacgc cgacgttaac tcgtttgccg
      901 ctcccgcaaa ttgcgaaaga acaagaacgc ccgggttggc tgggtcctga gcggcaacat
      961 actcttttgc taccaggttc atcccgtcac gcagtggcgt cactaagccc acgtcagagt
     1021 agcggaatat tttcatcagt aatttacggt caaaatgctg attcaaataa taaagcggcg
     1081 tccagcctaa ttgcccgtat ttaccattaa ttcgtccggc ttcattttcg agctgatgac
     1141 gaatatcctg ataggcttgc acatcaccac gcgacgttgg tgcaatctgg gtataacgaa
     1201 ttttaccatg atgctgcgga tatttttcca gcaacgcttc ataggcgaga aaacgctctg
     1261 gcaaaccttt ggaataatcc agccgttcga cagaaaagat attttgtacg tttttcagtt
     1321 ccgctttaag ttgcgccagt tttggcggca gtggcccggc agcctgtttg gctatttctt
     1381 ttggctcaat gccaatcggg taaacttctg ttcgaaacgc tttgccccag gctgtatggc
     1441 tttttgcgct acgtgtcgtg acgcgggtca ggttagaaag gcaatccagg aacgccagac
     1501 gatcgttttc tgtctggaaa cccagcaaat cataatcaca aagctgttca agcaaggtgt
     1561 catatgtcgg cagcgcgttg aagatttccg gtgtcgggaa aggaatatgc agaaagaaac
     1621 caatgcgatt attcactccc cgtttgcgta attcatgcgc aaatggcaac aggtgataat
     1681 cgtggatcca gataatgtca tcgtcttgca acagcggcag taatttatct gccagcaacg
     1741 catttacgcg tagatagccg tcccaggcag gacgctgaaa ttgcaccaga tcgaggcgat
     1801 aatgaaaagc aggccagaga acggcattgg agaattggtt gtagtattcg tcaaggtcct
     1861 gttcgctgag gttaaaagag gcccacgtaa tgttaccctt tttcaccttt tttagcggct
     1921 gatcctcatt ccctgtttca ccactccagc caaaccacag tccgcctgcg gctttcagtg
     1981 cccccagtat gccaacggca aggccaccgg cactggcggc gtgctcatct ggtggtgcaa
     2041 tccggttaga tactacgact aaacgactca tagtcatcac tcctgttatt ttctcttttt
     2101 tgttgtaatg cggtggttat catttcaagc cagctccaga catccggtac gcccgccagt
     2161 cgccatgatg cctgagttgc gcctgtgcca atttttactg acattccgcc cagtcggtta
     2221 acgactgcga agccagattc atcggttaaa tcatcgccca gaaatacggg cgttcgcccg
     2281 ataaagggag cttcctgcat aaaggcagca atcgcctcac ctttactggt acctctcggt
     2341 ttgatctcga caacacactt tccctgctgt aacgccattt gtggccagat ctgagtaata
     2401 cgttgcgcta atgtcattaa tgcgtcttca tgctgcggag cctgacgata atgcagcgca
     2461 aaagccatcc ctttcgtctc cagctccgca ccgggatact gggcgatgac cgtatgcagt
     2521 tgcacgttaa tatcacgtgc aatcgcatcc ggcagatgaa cgatatgcgt tttaccattg
     2581 atgtcacggc gctccgcccc atgcacgccc gctaacggga agcgataagg ttttgccagt
     2641 gcgtccagct ccaccattga gcgccctgat atcaatgcca atgcaccatc acttgcggtt
     2701 gccagtagct gaagtccttg cagaatattg tcaggtacga cgacctgatc gggatgcggt
     2761 ttgatttccg ccagcgttcc atcgagatca aaaaaccagg catatttcgc ggatagttca
     2821 ggggtttcgg ttaacggttc tgtcacccgg ttctcctcct tctcttttct tattgtgttg
     2881 cgcagaacgc gctttacaga catgtaagta tagacagtgt gacgggggtc gccatttgca
     2941 acgaagaaca gccaaccgtt gtggcggctg gctgtggtgg gaaaaaacgt taaattgttg
     3001 tggaaaaaag catcagacag tgcgtttcgc tttttgcttg taacggtcga agatcaccgc
     3061 tgccagcagg attaagccgc gaaccacgta ctgcgcgaaa ggagaaatat taagcaggtt
     3121 catggcgttt tccacggtgc ctaaaattaa gatacccgcc accacatatg agatttttcc
     3181 gatgccacct ttcagagaaa cgccacctaa aacgcaggcg gagataacaa taagctcata
     3241 accaatcgac gtcattggct gcccactggt catacgtgaa gccagaataa ttccggctat
     3301 cgcagatacc aggccagaga gaacaaagat aataattttg gtgcgagcaa ccggtacacc
     3361 cgccagacgc gcagcctctt cgttcccgcc aatcgccagg gtattacgac caaaggtagt
     3421 tttattcagc agcaaaccaa agataatcag acacgcgacg gtgagccaga ttggcgcagg
     3481 cagaccgaac cagttggcgt aaccaagggc aaagaagctt tcatcttcga taccgaccgc
     3541 tttaccgtcg gaaatgatgt acgccagacc tcgaacaatc tgcatcgttg ccagtgtcgt
     3601 gatcagagca tttattttca gtttggcgat aacaaagcca ttgaccaggc cacagagaat
     3661 gccaagcagc aaccccgctg ccacgccaat ccacaggctt tcagtcagat tgataaccac
     3721 cgccgtggtg acacccgcac aggcaattac ggaggcgaca gaaaggtcaa agtcaccgga
     3781 agcaaggcag aacaacatgc cacacgccac catccccgac atggaaattg ccaggcccaa
     3841 ccctttcata ttaataaagg tggcaaagtt cgggacaaaa atggcacagg cgataaaaag
     3901 caccgcaaac accaccagca tgccgtactg atcccagata cgcccgaagc tgaatgacga
     3961 cttaggtgcg ccagaacccg atgtagaaac agaagacatc atactctcct tactcaggca
     4021 actgcctggc tgactttagg catcgcaagg ctcagtgcct gacgctcatc tgcctgctcg
     4081 tgtaacaatt caccggcgat ttcaccttcg cgcatcacca caatccggtc ggcaacgccg
     4141 aggacttcag gtaagtcgct ggaggcaaac agcaccgcca caccctgcgc cgccagcgca
     4201 taaatcacgt tatatatttc gtgcttagca ccaacatcaa tgccgcgcgt aggttcatcc
     4261 agcaaaatga ccttcatctc ttccgataac cagcggccca gaatggcttt ttgctgattt
     4321 ccacctgaga gattcataat cagttgctca gcgcccggcg ttttgatgtt gagcgaacga
     4381 atgtggtgat cggcattgtt ttcttcccaa ccgttgttga ttacacaacc gccgagcaca
     4441 tgtttacgtc tggcactgat gttgatattg tcgcgaacgg agtgcacagg aataatgcct
     4501 tccgctttgc gatcttccgg gcagagcatc atgcctgcgg caatggcgtg gctcggttta
     4561 cgaatatcga tcggctgttg gtcgatataa acctgaccgg cggtgatttg cgtcccgcca
     4621 aacatgcctt tcattaattc gctacgcccc gcccctacca gaccaaacag cccaacaatt
     4681 tcaccactgc gaaccgccag acttattggc gtacgcacgc ctggtgcttt cacagcatca
     4741 agacgcaggc gctcctcgcc ataactacgc ggttgccagc cgtagatatc gccaatgtcg
     4801 cgcccgacca tcgcctgcac cagcgcgtcg tggtcaacct gctgcatatc ggtaaaggtt
     4861 ttgacataac gtccatcttt aaagacagtg atggcatcgc tgagggcaaa tatttcttcc
     4921 atacggtgag aaacgtataa gatcacccgc ccctcttttc gcagttcacg aataacgcgg
     4981 aaaagattgt cgatttcgcg ggcagaaagg gagctggttg gctcatcaaa ggcgataatt
     5041 ttagcgttac gcgccagcgc cttggcgatt tcaaccatct gccactgacc aatggagaga
     5101 tatttcagcg gcgtgtccgg gtcaatatcc ataccaagat gtttaagttg taaacccgcc
     5161 tcataattca gcaatgagcg attcacaatg ccgcctttat gcggcagctg gccgagatag
     5221 atgttttccg cgacggtcat ttccggcacg agatgcagtt cctggtaaat aatcgccaca
     5281 cccgcgttaa gtgctgcggt cgtgtcggaa aaggacattt cctgcccatt aatcactaca
     5341 gaacccgtgg ttggcgcata gttgccgctg aggattttta agagagttga ttttcctgcg
     5401 ccattttcac ccatcaacgc atgaacctga ccggcatagc agtcaaaact aatatccgtc
     5461 agcgccttaa cgccgggaaa cgttttaccg atgccgcgaa atgagagata cggggtagac
     5521 tgttgcataa cgtctccgtg aatcactggt cgttcacacc ccctctgcat catgcaaagg
     5581 ggaatttttc cggcaaatta cttaccgcct aaaccttttt tctccagttc ttctttaaag
     5641 ttgtcacgcg tgatcagcac cacgtcggta acttcggtga atttggtcgg ttcaacacct
     5701 tttgctaccc agttgtaaag catttcgctg gacttataac catgtacgtc cgggcttggc
     5761 aacagggaac cgtagaagcc ggttgcctgt gccttagaca gttcgctcac cgcatccaca
     5821 ccgttaatac cgataccgat gatatcggcc gctttaaagc cctgaccttc cgtcgcgcgt
     5881 acgccgccca gcacggtgct gtcgttcata ccgacgatca gccaatgttt aacttccgga
     5941 tgttgaacca gcattgagtt ggcagcgtca aatgccccgg ggatgtcgtt agatttggta
     6001 ggtacctgat aaatttgttt ttccgggaat cctgcagctt tcagcgcatc catagaaccc
     6061 gtagtacggc gacgagccgt atccagttcg ttggaggtaa tcgccatcac cgcgctttct
     6121 ttgacatccc agccacgttt ctgcatctct ttatacagtt cctggccctg acgttcgcca
     6181 attttggtcg ccgccatcat caccagagga acggtatcca ttggcttacc tttggcgtta
     6241 acaaactggt catccacggc aatgactttc atatcgtagc cacgcgcttt cgccacgatg
     6301 gcagagccga gtttcgggtc cggagtacaa ataacgaaac cttttgcgcc actggcggcc
     6361 aggctatcga tcgcgttcaa tgttttttcg ccatccggca cggcaatctt aataacctca
     6421 aaccctaaat ccttcccggc tttatcggca aacttccatt cggtctggaa ccacggctct
     6481 tccggttgct tcaccaggaa accgagcttc aggttctccg ccatagcgga ttgtgacata
     6541 acggctgcca gaccaatggc tgccagggct ttagtaaatt tgtgcatggt tctctccagc
     6601 tttagtgtcg ttttgtgcag ggcaaaaacg aatgacattc gttaaattaa tcggaaaaca
     6661 aagcattacc ttttaactaa aagataagtg actgtgttga catagtttta gcgagaaatt
     6721 aattctccat aggagagcaa tatcacatcg cagaattaca gtgagaacgt gcataaattt
     6781 agcgggaaaa gacataagga aaagccaatt tgtcagacaa attgttgaat gcacagcaga
     6841 ttaatccata agattagcct ggaaatcctt gttgtctttg gtacccatgc gggatgtctt
     6901 ctttttcacc agtcaatagg ccgcattacc tggcgttgag tttttgaaat ggtgtaataa
     6961 ccgcaactca aagatgtgga aaatgcacgt cattcatttc gtcattaatt atcactgtgc
     7021 tcattaatta acagaacacg tataatgaga gccatctcgc aaaaatgaaa aaacgtttta
     7081 taaaataatc acttcatcat gaattcaaat tcattgatta atatcaacaa gatacaaaaa
     7141 gcactatcat taaaattcat tgcagttaca ttgatttcat caatgaaatg taaaaatata
     7201 taaacttgat gatttaagca ttttcttata cccgttcaga cgttattctt atttcagatc
     7261 atcgtcagaa ttgactccac gatcacattt cggaccggca gaaaggaatt attctgcaaa
     7321 cagtaattat ggtgttttga tttatcttgc acctctccac ttctggatat aaggatatta
     7381 ggtatggcaa ccgctggaat gcttctcaaa ctcaactctc aattgaaccg cgagttttac
     7441 gcatccaatc tctaccttca cctgagtaac tggtgttctg aacagagtct gaacggcacc
     7501 gccactttcc ttcgcgccca ggcacagagt aatgtgaccc aaatgatgcg catgtttaac
     7561 tttatgaaga gtgtcggcgc tacccccatc gttaaagcca ttgatgttcc cggtgaaaaa
     7621 ctgaactctc tggaagaact gttccaaaaa acgatggaag aatacgagca acgttctagt
     7681 acgttggcac agttagccga tgaagcgaaa gaactgaatg atgattcaac cgtcaatttc
     7741 ctgcgcgatc tggaaaaaga acagcagcat gatggcctgt tgctgcaaac cattcttgat
     7801 gaagtgcgca gtgcgaaact tgcgggtatg tgcccagtgc agaccgacca acatgttctg
     7861 aatgtcgtgt cgcatcagct gcattgatca tacatcgacg ctaatgcatt gcaccgatga
     7921 agtttttgag aaaccgtgcc tcatcttttt tgaagcagcg gttttttaat gggattcgcc
     7981 ttgtggggta aacttgagct caatcagcgc gatggctttt tggattgccc gcatggtgac
     8041 cgggtctgcg gcagcgggat gatgagtaaa gtcgatattc ttcagctgac tggacatttt
     8101 ttcacgaact tcaacgggcg cgattacatc gagaacatcc agaatttgtt tgataaccaa
     8161 ctggcaagca accacatcag aaaccagttc ctgatcggca ttcagcgtct gggacatcgt
     8221 aaactcctga tagcattttg aaagccgtta tagtagcggc ttcacatctt caacgatagt
     8281 cacatccacc ctcatcagga cacaaaaaaa cctgccggag caggtttttt gttatcggaa
     8341 catattgcct ggcggtacgt ctttgaacgt cttgcaatag ttattgaaca tacttttcag
     8401 gattttgcgc agtttcatcg cggcactccg accatttgtt atacaggtgt tat
//
LOCUS       DBFIFM010000187         8421 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.187, whole genome shotgun sequence.
ACCESSION   DBFIFM010000187 DBFIFM010000000
VERSION     DBFIFM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.187"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            393..1370
                     /gene="glaH"
                     /locus_tag="N7C74_003829"
     CDS             393..1370
                     /gene="glaH"
                     /locus_tag="N7C74_003829"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HGU2433620.1"
                     /translation="MNALTAVQNNAVDSDQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPMARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIEISKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1390..2658
                     /gene="lhgO"
                     /locus_tag="N7C74_003830"
     CDS             1390..2658
                     /gene="lhgO"
                     /locus_tag="N7C74_003830"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HGU2433621.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKSGVVIRTRQGSDYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2681..4129
                     /gene="gabD"
                     /locus_tag="N7C74_003831"
     CDS             2681..4129
                     /gene="gabD"
                     /locus_tag="N7C74_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HGU2433622.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPVWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4143..5423
                     /gene="gabT"
                     /locus_tag="N7C74_003832"
     CDS             4143..5423
                     /gene="gabT"
                     /locus_tag="N7C74_003832"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HGU2433623.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5661..7061
                     /gene="gabP"
                     /locus_tag="N7C74_003833"
     CDS             5661..7061
                     /gene="gabP"
                     /locus_tag="N7C74_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HGU2433624.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7082..7744
                     /gene="csiR"
                     /locus_tag="N7C74_003834"
     CDS             7082..7744
                     /gene="csiR"
                     /locus_tag="N7C74_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HGU2433625.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7745..8194)
                     /gene="kbp"
                     /locus_tag="N7C74_003835"
     CDS             complement(7745..8194)
                     /gene="kbp"
                     /locus_tag="N7C74_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HGU2433626.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8278..>8421)
                     /gene="yqaE"
                     /locus_tag="N7C74_003836"
     CDS             complement(8278..>8421)
                     /gene="yqaE"
                     /locus_tag="N7C74_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HGU2433627.1"
                     /translation="IVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIHAFWVQ
                     TRD"
BASE COUNT         1880 a         2192 c         2370 g         1979 t
ORIGIN      
        1 gtagcgctct tcttttaggg aaaacaggag aaccgctgaa ctatctctat ttgtaattta
       61 atgttatatc tgccccgcta aaacggggca gataatatat ttagtttact agcggtcatt
      121 ttgcagtgaa gccatttacc gttttttatc gaccagataa tctgttctct gatggtaatc
      181 cccccctaac ctgttgcttt agttattcat ttcctgtctc actttgcctt aataccctac
      241 gttaaatgtt accaatttgt tgctttttat cacaataaga aaacaatatg gcgcatttgt
      301 gcgcattttt cagaaatgta gatattttta gattatggct acgaaatgag catcgccatg
      361 tcaccctaca tctcataaga ggatcgcttc tgatgaatgc actgaccgcc gtacaaaata
      421 acgctgtcga ttcagaccag gactatagcg gattcactct catcccgtcg gcgcaatccc
      481 cgcgtctgct ggaactcacc ttcaccgaac agacgaccaa acagtttctc gaacaggttg
      541 ccgaatggcc cgtgcaggcg ctggagtaca aatcgtttct gcgttttcgg gtaggtaaaa
      601 ttcttgacga tctgtgtgcg aaccagctgc aaccattgct gttgaagacc ctgctaaacc
      661 gtgctgaagg tgcgctgttg atcaatgcgg tgggtgtcga tgatgtgaag caagcggatg
      721 agatggtgaa gctggcgacg gcggtggcgc atctgattgg ccgttccaat ttcgatgcga
      781 tgagcggtca gtattacgcg cgttttgtag tgaaaaatgt cgataactca gacagctatc
      841 tgcgtcagcc gcaccgcgta atggagctgc acaacgacgg cacctacgtc gaagagatca
      901 ctgactatgt gctgatgatg aaaatcgacg agcagaacat gcagggtgga aattcgttgc
      961 tgctgcatct cgatgactgg gaacatctgg accactattt ccgccatcca atggcgcgtc
     1021 gcccgatgcg ctttgccgcg ccgccgagca aaaacgtcag caaagatgtt ttccatccag
     1081 tgttcgacgt tgatcaacag ggtcgcccgg tgatgcgcta tatcgaccag ttcgtccagc
     1141 caaaagactt cgaagaaggc gtgtggttga gcgagctttc ggacgccatt gaaatcagta
     1201 aaggcattct ttctgtaccc gttcccgttg gcaaattcct gttgattaac aacctgttct
     1261 ggctgcacgg tcgcgaccgc tttactccgc acccggatct gcgccgtgaa ctgatgcgtc
     1321 agcgtggcta tttcgcttac gccactcacc actatcagac gcatcagtaa gcgcaaagga
     1381 attgagcgga tgtatgattt tgtgattatt ggcggcggca tcatcggcat gtcgaccgcc
     1441 atgcaactga ttgatgtcta tccggatgcc cgcattgcgt tgctggaaaa agagtccggc
     1501 ccggcctgtc atcagacggg ccacaacagc ggcgtgatcc atgccggggt ttattacacg
     1561 cccggtagcc tgaaggcgca gttttgcctg gcgggaaacc gcgccactaa agccttttgc
     1621 gatcaaaacg gcattcgcta tgacaactgc ggcaagatgc tggtcgccac ctctgaactc
     1681 gaaatggaac ggatgcgcgc gttgtgggaa cgcacggcgg caaacggtat cgagcgcgag
     1741 tggttaaacg ccgatgaact gcgcgagcgc gaaccgaata tcaccgggct tggcggtatt
     1801 tttgtgccgt ccagcggcat tgtcagctac cgcgaagtca cggcggcgat ggcaaaaatt
     1861 ttccaggcca gaggcggcga gattatctat aacgccgaag tcagcggcct tagtgagcat
     1921 aaaagcggcg tggtgatacg tacccgtcag ggcagcgact atgaagcatc gacgctgatt
     1981 agctgttccg ggctgatggc tgaccggctg gtgaaaatgc tcggcctcga accgggcttt
     2041 attatctgcc cgttccgcgg cgagtatttc cgcctggcgc cggagcataa ccagattgtt
     2101 aaccatctga tttaccccat tcccgacccg gcaatgccgt ttttgggcgt gcatctcacc
     2161 cgcatgatcg acggcagcgt caccgtcggg ccaaacgcgg tgctggcttt caaacgcgaa
     2221 ggctatcgca agcgcgactt ctcatttagc gacacgctgg agattttggg ctcgtcgggg
     2281 attcgccggg tgctgcaaaa ccatctacgc tcaggactgg gcgagatgaa aaactcgctg
     2341 tgcaaaagcg gctatctgcg gctggtgcaa aagtattgtc cccggctttc gttaagcgat
     2401 ctccagccct ggcccgccgg tgtgcgggcg caggcggtat cgccggacgg caagctgatt
     2461 gacgattttc tgtttgtcac caccccgcgc acgatccaca cctgcaatgc gccctccccg
     2521 gcagcgacat cagcaattcc tattggtgcg catattgtca gcaaggtaca aacgctgttg
     2581 gcaagccaga gtaaccccgg acgcacgctg cgagcggcac gtagtgtgga tgccttacac
     2641 gccgcgttta atcaataacc tttgaaaaca ggatgtagcg atgaaactga acgacagtaa
     2701 cttattccgc cagcaggcgt tgattaacgg ggaatggctg gacgccaaca atggtgaagt
     2761 catcgacgtc accaatccgg cgaacggcga caagctgggt agcgtgccga aaatgggcgc
     2821 tgatgaaact cgcgccgcta tcgacgccgc caaccgcgcc ctgcccgtct ggcgtgcgct
     2881 caccgccaaa gaacgcgcca acattctgcg caactggttc aatttgatga tggagcatca
     2941 ggacgattta gcgcgcctga tgaccctcga acagggtaaa ccgctggctg aagcgaaagg
     3001 cgaaatcagc tacgccgcct cttttattga gtggtttgcc gaagaaggca aacgcattta
     3061 tggcgacacc attcccggtc atcaggccga taagcgcctg attgtgatca agcagccgat
     3121 tggcgttaca gcggccatca cgccgtggaa ctttccggcg gcaatgatta cccgcaaagc
     3181 cggtccggcg ctggcggcag gctgcaccat ggtgctgaag cccgccagtc agacgccgtt
     3241 ctctgcgctg gcgctggcgg agctggcgat ccgcgcgggc attccggctg gcgtgtttaa
     3301 cgtggtcacc ggttcggcgg gcgcggtggg taatgaactg accagcaacc cgctggtacg
     3361 caaactgtcg tttaccggct cgaccgaaat tggccgccag ttaatggaac agtgcgcgaa
     3421 agacatcaaa aaagtgtcgc tggagctggg cggcaacgcg ccgtttatcg tctttgacga
     3481 tgccgatctc gataaagccg tggaaggcgc actggcctcg aaattccgca acgccgggca
     3541 aacctgcgtc tgcgccaacc gtttatacgt gcaggacggc gtgtatgacc gctttgccga
     3601 aaaattgcag caggcggtga gcaaactgca tatcggcgac gggctggata aaggcgtcac
     3661 catcgggccg ctgatcgatg aaaaagcggt agcaaaagtg gaagagcata ttgccgatgc
     3721 gctggaaaaa ggcgcgcgcg tagtttgcgg cggtaaagca catgaacgcg gcggcaactt
     3781 cttccagccc accattctgg tggacgtgcc ggccaacgcc aaagtgtcga aagaagagac
     3841 cttcggcccc ctcgccccgc tgttccgttt taaagatgaa gccgatgtga tcgcgcaagc
     3901 caatgacacc gagtttggcc ttgccgccta tttctatgcc cgtgatttaa gtcgcgtctt
     3961 ccgcgtgggc gaggcgctgg agtacggcat cgtcggcatc aataccggga ttatttccaa
     4021 tgaagtggcc ccgttcggcg gcattaaagc ctcgggtctg ggtcgtgaag gttcgaagta
     4081 tggcatcgaa gattacttag aaatcaaata tatgtgcatc ggtctttaac tggagaacgc
     4141 gaatgagcag caataaagag ttaatgcagc gccgcagtca ggcaattcct cgtggcgttg
     4201 ggcaaattca ccccattttc gctgatcgcg cggaaaactg ccgggtgtgg gacgttgaag
     4261 gccgagagta tcttgatttc gcgggcggca ttgcggtgct caataccggg cacctgcatc
     4321 cgaaagtggt tgccgcggtg gaagcgcagt tgaaaaaact gtcgcacacc tgcttccagg
     4381 tgctggctta cgagccgtat ctggagctgt gcgagattat gaatcagaag gtgccgggcg
     4441 attttgccaa gaaaacgctg ctggttacga ccggttccga agcggtggaa aacgcggtga
     4501 aaatcgcccg cgctgccacc aaacgtagcg gcaccatcgc ttttagcggc gcgtatcacg
     4561 ggcgcacgca ttacacgctg gcgctgaccg gcaaggtgaa tccgtactct gcgggcatgg
     4621 gcctgatgcc agggcacgtt tatcgcgcgc tttatccttg cccactgcac ggcatcagtg
     4681 aagatgatgc tatcgccagt atccaccgaa tttttaaaaa tgatgctgcg ccggaagata
     4741 tcgccgccat cgtgattgag ccggttcagg gcgaaggcgg tttctacgcc gcgacgcctg
     4801 cgtttatgca gcgtttacgc gcgctgtgtg acgagcacgg gatcatgctg attgccgatg
     4861 aagtgcagag cggcgcgggg cgtaccggca cgctgtttgc gatggagcaa atgggcgtgg
     4921 caccagatct caccaccttt gcgaaatcga tcgcaggcgg cttcccactg gcgggcgtca
     4981 ccgggcgcgc cgaagtgatg gatgccgtcg ctccaggcgg gctgggtggc acctatgccg
     5041 gtaatccgat tgcctgcgtg gcggcgctgg aagtgttgaa ggtgttcgag caggaaaatc
     5101 tgctgcagaa agccaacgat ctggggcaga agttgaaaga tggattgttg gcgatcgccg
     5161 aaaaacaccc tgagatcggc gacgtacgcg ggctgggggc gatgatcgcc atcgagctgt
     5221 ttgaagacgg cgatcacaac aagccggacg ccaaactcac cgccgagatc gtggctcgcg
     5281 cccgcgataa aggcctgatt cttctctcct gcggcccgta ttacaacgtg ctgcgcatcc
     5341 ttgtaccgct caccattgaa gacgctcaga tccgtcaggg tctggagatc atcagccagt
     5401 gttttgctga ggcaaagcag tagcgccgct cctatgccgg aggcgacgct gcgcgtcttg
     5461 tccggcctac ggggatccag gtcggataag gcgtttacgc cgcatccgac aatctgtacg
     5521 tgaacaggaa taaatctatg ttggccgggt aaggcggagc cgctctccgg caaaaagaat
     5581 caataacaat tatacgcgtg acccgacgcg ggaaatgtcg gggcgctctc ccaagtgaca
     5641 cactttcgag aggattcagg atggggcaat catcgcaacc acatgagtta ggcggcgggc
     5701 tgaagtcacg ccatgtcacc atgttgtcta ttgccggtgt tatcggcgca agtctgtttg
     5761 tcggttccag cgtcgccatc gccgaagcgg gcccggcggt attactggcc tatctgttcg
     5821 ccgggctact ggtggttatg attatgcgga tgttggcgga aatggcggtt gccacgcccg
     5881 ataccggttc gttttccacc tatgccgata aagctattgg tcgttgggcg ggttatacca
     5941 tcggctggtt gtactggtgg ttttgggtac tggttatccc gctggaagcc aacatcgccg
     6001 ccatgatcct gcactcatgg gttccaggca ttcccatctg gttattttcc ctcgtcatta
     6061 cccttgcctt aactggcagt aatttattaa gcgttaaaaa ctacggcgaa tttgagttct
     6121 ggctggcgct gtgcaaagtc atcgctatcc tggcctttat tttccttggt gcagtcgcaa
     6181 ttagcggttt ttacccgtat gccgacgtga gcgggatctc aagattgtgg gatagcggcg
     6241 gctttatgcc caacggcttc ggtgcggtat taagcgcgat gttgatcacc atgttctcgt
     6301 ttatgggcgc agaaattgtc accattgccg ccgcggaatc cgacacgccg gaaaaacata
     6361 ttgtccgcgc taccaactcg gttatctggc gtatttctat cttctatttg tgctctattt
     6421 ttgtcgtagt ggcgttaata ccgtggaata tgccggggct gaaagccgtt ggatcttatc
     6481 gctcggttct ggaattgctc aatatacccc atgcgaaatt aatcatggac tgcgtgatat
     6541 tactttccgt aaccagttgc ctgaactcgg cgctgtatac cgcgtcaagg atgctctact
     6601 ccttaagccg tcgcggtgat gcgcccgcgg taatgggcaa aatcaaccgc agtaaaaccc
     6661 cgtatgtggc ggtgttactc tccaccggag cggcattttt aacggtcgtg gtgaactatt
     6721 acgcacctgc gaaagtgttt aaattcctga tcgacagctc cggtgctatc gccctgctgg
     6781 tttatttagt catcgccgtt tcacagttgc ggatgcgtaa aattctgcga gcagaaggaa
     6841 gcgaaattcg cttgcgcatg tggctttacc cgtggctcac ctggctggta atcggcttta
     6901 ttacctttgt gttggtggtg atgttattcc gcccggcgca acagttagaa gtgatctcta
     6961 ccggcttatt agcgataggg attatctgta ccgtgccgat tatggcgcgc tggaaaaagc
     7021 tggtattgtg gcaaaaaaca cccgttcata atacgcgctg ataatgatca ggagtcacac
     7081 catgaccatt acgtctctgg atggctatcg ctggctgaag aacgatatta ttcgcggtaa
     7141 ttttcaaccg gatgaaaaat tacgaatgag tttgctgaca tcgcgttatg cacttggcgt
     7201 tgggccgtta cgggaagctc tttcgcaact ggtggcggaa cggctggtca cggtggtgaa
     7261 tcaaaaaggg tatcgggtgg cgtctatgtc agagcaggag ctgctcgata ttttcgacgc
     7321 ccgcgccaat atggaagcga tgttagtgag tctggcgatt gcccgcggtg gcgatgagtg
     7381 ggaggcagac gttctcgcaa aagcgcatct gctgagtaag cttgaggcct gtgacgccag
     7441 cgagaaaatg cttgatgagt gggatctgcg tcatcaggcg tttcatactg caattgtggc
     7501 gggctgtggt tctcactatt tgctgcaaat gcgtgaacgg ttgtttgatc tggcggcgcg
     7561 ttatcgattt atctggctgc ggcgaacggt gctttcggtg gaaatgctgg aggataaaca
     7621 cgatcagcac cagaccctga ctgcggcggt actggcgcga gataccgcgc gcgccagtga
     7681 gttaatgcgc cagcatttac tgacgccaat tcccattatc cagcaggcga tggctggcaa
     7741 ttaactactc ttccggaata cgcaacactt gccccggata aattttatcc gggcttttta
     7801 gcatcggttt attcgcttcg aagattttat tgtacagatt agcgttaccg tagacctgtt
     7861 tggaaatggc actcagagtg tcgccagact taacggtata aaactggctg gcagtggctg
     7921 gtgtcgccgt tttcacctga tcatcaacac tggcaatacc ggaaatattc cccaccgcaa
     7981 caaggatttt ctccttcgcc tcctgactca ggccgtcacc agtgaccgtc gctttgccat
     8041 cggcaatttg aatgttcact ttatcggcat caggtatacc ggttttgctc agatgctcct
     8101 gcaccttctt cgcctgatca tctttatcgt gctgacctgt aaccgcgtcc cagagttttt
     8161 ctccggcatc tttcacaaaa ttgaacagac ccatagctac ctcattcgtt aacggaaaca
     8221 ctcagaaagt gtagtagagg aacgcggcgc gttaacgccg cggggatgag cacaggttta
     8281 atcgcgggtt tgcacccaga acgcgtgaat caaaccgggg atatacccaa gcagtgtcaa
     8341 cagaatatta ataatgaacg cccaaccgaa ccctttaccg agcagcacgc cgagcggcgg
     8401 cagaatgatg gtgatgacga t
//
LOCUS       DBFIFM010000188         8416 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.188, whole genome shotgun sequence.
ACCESSION   DBFIFM010000188 DBFIFM010000000
VERSION     DBFIFM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.188"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            110..2488
                     /gene="ppsA"
                     /locus_tag="N7C74_003837"
     CDS             110..2488
                     /gene="ppsA"
                     /locus_tag="N7C74_003837"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HGU2433628.1"
                     /translation="MSNNGSSPLVLWYNQLGMKDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2545..4190)
                     /gene="fadK"
                     /locus_tag="N7C74_003838"
                     /pseudo
     CDS             complement(2545..4190)
                     /gene="fadK"
                     /locus_tag="N7C74_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(4247..4540)
                     /locus_tag="N7C74_003839"
     CDS             complement(4247..4540)
                     /locus_tag="N7C74_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HGU2433629.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(4537..5826)
                     /locus_tag="N7C74_003840"
     CDS             complement(4537..5826)
                     /locus_tag="N7C74_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU2433630.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDILVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLADIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(5882..6820)
                     /locus_tag="N7C74_003841"
     CDS             complement(5882..6820)
                     /locus_tag="N7C74_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HGU2433631.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSSLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(6840..7604)
                     /locus_tag="N7C74_003842"
     CDS             complement(6840..7604)
                     /locus_tag="N7C74_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HGU2433632.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGKGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            7920..>8416
                     /locus_tag="N7C74_003843"
     CDS             7920..>8416
                     /locus_tag="N7C74_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HGU2433633.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENHLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENF"
BASE COUNT         1998 a         2160 c         2189 g         2069 t
ORIGIN      
        1 tatatttgct tgaacgattc accgtttttt tcatccggtt aaatatgcaa agataaatgc
       61 gcagaaatgt gtttctcaaa ccgttcattt atcacaaaag gattgttcga tgtccaacaa
      121 tggctcgtca ccgctggtgc tttggtataa ccaactcggc atgaaggatg tagacagggt
      181 tgggggcaaa aatgcctccc tgggtgaaat gattactaac ctttccggaa tgggtgtttc
      241 cgttccgaat ggtttcgcca caaccgccga cgcgtttaac cagtttctgg accaaagcgg
      301 cgtaaaccag cgcatttatg aactgctgga taaaacggat attgacgatg ttactcagct
      361 tgcgaaagcg ggcgcgcaaa tccgccagtg gattatcgac actcccttcc agcctgagct
      421 ggaaaacgcc atccgcgaag cctatgcaca gctttccgcc gatgacgaaa acgcctcttt
      481 tgcggtgcgc tcctccgcca ccgcagaaga tatgccggac gcttcttttg ccggtcagca
      541 ggaaaccttc ctcaacgttc agggttttga cgccgttctc gtggcagtga aacatgtatt
      601 tgcttctctg tttaacgatc gcgccatctc ttatcgtgtg caccagggtt acgatcaccg
      661 tggtgtggcg ctctccgccg gtgttcaacg gatggtgcgc tctgacctcg catcttctgg
      721 cgtgatgttc tccattgata ccgaatccgg ctttgaccag gtggtgttta tcacttccgc
      781 atggggcctt ggtgagatgg tcgtgcaggg tgcggttaac ccggatgagt tttacgtgca
      841 taaaccgaca ctggcggcga atcgcccggc tatcgtgcgc cgcaccatgg ggtcgaaaaa
      901 aatccgcatg gtttacgcgc cgacccagga gcatggcaag caggttaaaa tcgaagacgt
      961 accgcaggaa cagcgtgaca tcttctcgct gaccaacgaa gaagtgcagg aactggcaaa
     1021 acaggccgta caaattgaga aacactacgg tcgcccgatg gatattgagt gggcgaaaga
     1081 tggccacact ggtaaactgt tcattgtgca ggcgcgtccg gaaaccgtgc gctcacgcgg
     1141 tcaggtcatg gagcgttata cgctgcattc acagggtaag attatcgccg aaggccgtgc
     1201 tatcggtcat cgcatcggtg cgggtccggt gaaagtcatc catgatatca gcgaaatgaa
     1261 ccgcatcgaa cctggtgacg tgctggtcac tgacatgacc gacccggact gggaaccgat
     1321 catgaagaaa gcatctgcca tcgtcaccaa ccgtggcggt cgtacctgtc acgcggcgat
     1381 catcgctcgt gaactgggca ttccggcggt agtgggctgt ggtgatgcaa cagaacggat
     1441 gaaagacggt gagaacgtca ctgtttcttg tgccgaaggt gataccggtt acgtctatgc
     1501 ggagttgctg gaatttagcg tgaaaagctc cagcgtagaa acgatgccgg atctgccgtt
     1561 gaaagtgatg atgaacgtcg gtaacccgga ccgtgctttc gacttcgcct gcctgccgaa
     1621 cgaaggcgtg ggccttgcac gtctggaatt tatcatcaac cgtatgattg gcgtccaccc
     1681 acgcgcactg cttgagtttg acgatcagga accgcagttg caaaacgaaa tccgcgagat
     1741 gatgaaaggt tttgattctc cgcgtgaatt ttacgttggt cgtctgactg aagggatcgc
     1801 gacgctgggt gccgcgtttt atccgaagcg cgtcattgtc cgtctctctg attttaaatc
     1861 gaacgaatat gccaacctgg tcggtggtga gcgttatgag ccagatgaag agaacccgat
     1921 gctcggcttc cgtggcgcgg gacgctatgt ttccgacagc ttccgcgact gtttcgcgct
     1981 ggagtgtgaa gcagtgaaac gtgtgcgcaa cgacatgggg ctgaccaacg ttgagatcat
     2041 gatcccgttc gtgcgtaccg tagatcaggc gaaagcggtg gttgaggaac tggcgcgtca
     2101 ggggctgaag cgtggtgaga acgggctgaa aatcatcatg atgtgtgaaa tcccgtccaa
     2161 cgccttgctg gccgagcagt tcctcgaata tttcgacggc ttctcaattg gctcaaacga
     2221 catgacgcag ctggcgctcg gtctggatcg tgactccggc gtggtgtctg aactgttcga
     2281 tgagcgcaac gatgcggtga aagcactgct gtcgatggcg attcgtgccg cgaagaaaca
     2341 gggcaaatat gtcgggattt gcggtcaggg tccgtccgac cacgaagact ttgccgcatg
     2401 gttgatggaa gaggggatcg atagcctgtc tctgaacccg gacaccgtgg tgcaaacctg
     2461 gttaagcctg gctgaactga agaaataaaa taaatccccg gcggcgtttt gtcgccgggt
     2521 ttatgtgatc cccgaagatg aaacttattc aatctcttca cagacatcct gcgttaatcg
     2581 ccgcataata tctttccgta acaaaaactt ttgtatttta cctgaggcag tgcgcggtag
     2641 tttttcgatt accacgatat gttcaggata tttatatttt gcgacccgtt tacggctaaa
     2701 aaagccacca cctcttccag cgataatgaa tgatgcggcg ctttcagcac gacataagcg
     2761 catgatcgtt cacctaaacg ttcatcgggc attgcaacca cacaggcatc gtgaatttta
     2821 ggatgctgca ataaaatatc ttccacttca cggctgctaa tattttcgcc gccgcggaca
     2881 ataatatctt ttttgcgtcc ggtaattttt atatagccag cctcatccat acggcagaga
     2941 tcgccgctgt aataccagcc ttcttcatcc agggcacggg cggttaattc aggttcatca
     3001 aaatatccca taaacacatt ggggccacgc gaggcttctt caccttcgca acctggcggt
     3061 aaggtcttgc gtgcgtcatc gaccacttta atctctacac ctgcggcagc gtaaccatcg
     3121 gtgtgcataa agcgcgacaa aggatcatcg agattcacca ccgcatgcgg cgaactttct
     3181 gtggaaccat aaacacttaa taatttaatg ccgcgctgct ggcattcacg cgccactttt
     3241 ttggggattg tggtaccgcc gcaaagaaag aagcgcagcg ctgaaaggtc tgcgggttgt
     3301 ttctctagta aattcaaaag atcatagaca aacggcgttg cgccgagcat acaggagcaa
     3361 cgctgctgct caagcagcgc gagacacgca tcaggagtga aaatatctaa caacacgctg
     3421 cgagcgccaa ttaagaatgg tgccgttacg ccatgcagaa agcccgttgc gtgaccaagt
     3481 ggcgcaggca tcataaagac atcctgccag gttagattca gccgcgcgca ataagcccgc
     3541 tcactggcga gaatattgtt atgcgtaagc atcacgccct ttggcagacc ctcggttccg
     3601 gaggtaaaca gcaccgcagc taattcatcg ccgtgggtcg ttatcgccgt ggtcagtggg
     3661 gtattgtcgg cgataatctg acttaatgag agggaagagg tggcgggagc cagtttgtcc
     3721 acgccgacaa tttgttgtag ttgtggaagc tgattttgca gcggcaggat taaatctacc
     3781 ggacgcgttt gtttaaacaa cgtcggtgta aagaacattt ttgcctgaca cttattaagc
     3841 acccatacca gttctgcttc ccgccaggaa ggcaacagcg gtacggaaac cgcaccgatt
     3901 ttcaggcagg caagatagat aacggtaaat tcacaccagc ctggcagttg aaatgctatg
     3961 cgatcgcctg attcaatacc cttcgctaac atccagtttg ccagacagct cgcggcgtga
     4021 tcgagcgcgc tataggtgta cgatgctcca tgattatcga ccacggcaat tttgtctggc
     4081 atcgcacgag cggtctgctg ccagtaatcg gccagcgaag catcgcccca tagcccttgc
     4141 tgacgatacg ccgcacgacg ttgttcgtta aacgttaatg tcactttcat tttggactct
     4201 cgaaacagaa catcaggccc gagatgcggg cctgtgggat gcatgattag ccgtagcgaa
     4261 aatcaatacc gaaggtgcct gcgggatatt gccactgttc gagaatagtg ttaccgcaca
     4321 gcacccgaca ggtgccgcac tccagacaac cggcggaatc aaaatgaatg tttcctgcgt
     4381 catcctgctt ataaagtccg gcagggcagg ctttcattaa tttacggaat tcattgatat
     4441 cgggatttgc cgccaaaatg atatgcgggt ggccctcatc aacatggaat ttattgacgc
     4501 ctaatttgat gtcgacgtta accgtagcgt tctggctcat agcgcggttg ctcccttaat
     4561 gccatctttc agcaagttga tcagcccaat tttcttcgcg tgtcccatga tcattttgcg
     4621 taccggctgg ttaggcttac cgtcaatggt gaacatctcg ttcatgatgt cggctaccat
     4681 tcgtgggtat tggctaaaca ggcgcgggtt ttccatcagc gccgggatct tgcgaaaatg
     4741 ctgcatatca cgcatgacgc agctttgttc cagctcacgt ttgtattgcg ccagactgct
     4801 ggcggagaaa tccgcgcgtt ctttggcggc gatcactgtt gtggcggcag cctgagccga
     4861 tgcaatggct aaatccatgc cgcggaccgt aaaacccaaa ttcaggcaga agcctgcggc
     4921 gtcaccaacg atcatcacgc catcgttaac cagttgcggt accatcgcca gaccgccttc
     4981 tggcaccata tgcgcggaat attcaagcag tttgccgccg ctaatcagcg ggcgaatggc
     5041 ggggtgttgt ttaaaatctt ccagcatttg cggcacgctt ttttgcgcat gggcgatatc
     5101 agccaatcca caaaccagcc ccaaggatat ggaatccttg ttggtataga gaaatccccc
     5161 gcccatcagg ccgtcagaag gggaaccggc aaacagccag gcggcacctt cctcgcccgt
     5221 aatattaaag cgatcgttga tctgttctgg tgtgaggcca ataacctctt taacaccaac
     5281 agcgtaatga tgcggatcgg aagcgggaac cattcccagc gagcggccaa gcatcgagtt
     5341 aacgccatcg gctaaaatca ccacattggc ttcgagaata tcatccccgg cctggactcc
     5401 agtgaccttg tttccttcac gaaccaacgc atcgacgcga actcccggga taaactgtgc
     5461 gcccgcctgc tcggcttgtt ccatcaacca cgggtccaga cgattacgca atacggtata
     5521 agatgcgtgt tgcggaacat ctggttgctc gcggtgaaaa tcgagggtaa cggcgctctc
     5581 ttcggttaag aaggagattt tctcgcgtgt gaccttgcgt tctaccggcg ctgatgctgc
     5641 aaagcctgga atgattgctt caagtgtgtg ggcataaaga cgcccgccgg tcatgttttt
     5701 acatccggca ctgtcgccgc gttctatcac caggatatcc agcccggctc gcgccatgac
     5761 cagtgcggca acgctaccag caacgcccgc accgaccaca atggcatcaa atttgtcatc
     5821 cgacatgcgt tatgctccct tcccgctgcg tttacagcgg gagatgtggt tgaaagggga
     5881 attagcggct caactggcta atcagggcag ggacgacttt gtagatatcg cccaccagac
     5941 cgtagtcggc atagttgaag attggcgcat ttttatcttt attgatggcg acaatcactt
     6001 ttgcgccgtt gccgccaacc atatgctgga tctgcccgga gatccctagc gtcaggtaga
     6061 gatcggattt cagcaacacg ccggagacac cgatataacg ttcacgctcc atccagttct
     6121 cgccttcggc aattggacgt gaacagccga cttcagcatt cagcaccgcc gccagttcgt
     6181 ggaccatttt caggtcatcc tgcgctgcca gaccacgacc gacgccaacc acacgttttg
     6241 ctttgctcag gtccacgctg cttgcggctt tcgcacggcg ttcgcgacag agaatttcat
     6301 gacgcggagc aacgtaaggt accgtttctg tcgggcactg atgagaggta tcactggtgc
     6361 acggttcctg aacaccgggt gcaagggtaa tgatcgccag cgagctgttg atcttttcct
     6421 gagcgaacgc taacccgcca tacatccggt gttcggcgca aatgtgacca tcgacaatat
     6481 ccaccgccgt ggcatcgttc accagcgccg cattcagttg cacacttaac cgtgctgcca
     6541 gcgctttacc acgtttcgtc gcggccagta gcaacatagc ggggtgttta tctttcagca
     6601 gagcagcaat gctttcggcg taattttcag tgcgttgcag cgcgtcgttt tgctcaagaa
     6661 cataaataca ttttggacca taaggcataa ctgcctgcgc ctggtcggta ttttgtacaa
     6721 tggcatacac ctgttggccc cattgctgag cgccgccaaa cagttcagca taacgttcag
     6781 gattatcgct aaagacccag acgctgttta attgactcat cgtttcttct ccgtaggctt
     6841 cagttcaggg ctttcttcag atgttccgcc agctcggcaa tggcctccgg cgaatcgtta
     6901 tcgagaatga tgtgcttacg ttctgtttgc ggcggtacgc gaatgccggt cagttcagca
     6961 agtggtgcgc tctggctcca gccaatatca cttgcctgcc actgatttac cggtttttta
     7021 cccgcgccga gaatggcttt catcgaagga atgcgtggca cgttaatatc ggaggtgacg
     7081 cagagcacgg ctggaacaga gagttcaata acttcaacgt catcttcaag cgtgcgttca
     7141 atcaccagtg tattgccctg acgctgaata gcactcactg cattaatcac cggaagttgc
     7201 agaatttctc cgaccagcaa gccaacctgc tgggcataaa ggtcgccgga acctttacca
     7261 aagatcagta aatcgaagcc gatcttttca actgctgccg ccagcgcttt tgcggtatcg
     7321 agaggcagtg catgctcaag ttgtgcatcc tgcaccatat acaggctgtg cggcccgcgg
     7381 gatagcacgt ctttgcgcac tttcgagttc tgcaacaatg agccgccaac ggtcagcgcg
     7441 gctatctcat cgtcatctgt tgcaagctgg cttgcagctt caatggcatt gagatcgaac
     7501 tggctgattt tggcgtcggc attgtcgaaa ttcagggtgt attctggagt gacaacaatg
     7561 tcctgttctt caggcaccag cttaaagcag gttattattt tcatgatttc tcctgtgagt
     7621 cgcatacgga accatctccg atggaaggta aaaatggcct gaaaatagtg tgcgatagcg
     7681 gtgcggggtt ttcgacggag caagctgttt tccttgccaa caatcttgca aaaaaaatgc
     7741 gatcgctaaa acaaaagtgc aatttgcggc aatatagtta ttccatgacc gtacaatctt
     7801 gcattcagtg taaataatct tgtttagctc tcgctaaacg cggttgtata accgcaatac
     7861 acgccgtatt ttatccggca tatattgttt ttgcttgctc atggaatagg gaggttgtca
     7921 tgtatcaacg ctgttttgat aatgccagtg aaacgctgtt tgtcgctggt aaaacgccac
     7981 ggctttcacg ttttgcattt agcgatgatc caaaatggga gtctggacat cacgttcatg
     8041 acaatgaaac cgagctgatt tacgtcaaga aaggggtggc aaggtttacc atcgattctt
     8101 cgttatatgt cgcgcatgca gatgatatag tggtgataga acgcggcagg ctgcatgcgg
     8161 tggcctctga cgttaacgat ccggcaacga cgtgtacttg tgcgctgtac ggctttcagt
     8221 ttcagggggc tgaggaaaat catctactgc aaccgcattc ttgtccggta attgccgcgg
     8281 ggcagggaaa agaagtcatt aaaaccttat ttaatgagct aagtgtgatt ttgccgcaaa
     8341 gtaaaaatag ccaaacatct tcgttatggg atgcatttgc ctatacatta gcaattcttt
     8401 actacgaaaa ctttaa
//
LOCUS       DBFIFM010000189         8353 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.189, whole genome shotgun sequence.
ACCESSION   DBFIFM010000189 DBFIFM010000000
VERSION     DBFIFM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.189"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(53..301)
                     /locus_tag="N7C74_003844"
     CDS             complement(53..301)
                     /locus_tag="N7C74_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGU2433634.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESSDEWFVH"
     gene            complement(363..1460)
                     /locus_tag="N7C74_003845"
     CDS             complement(363..1460)
                     /locus_tag="N7C74_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2433635.1"
                     /translation="MAYYNIEKRLKSDGTPRYRCNVIIKEKGVITYRESKTFPKHAHA
                     KTWGTQKVMELDLYGIPSSNAVDGLTVRDLLHKYLNDPNAGGKAGRTKRYVLELLMDS
                     DISAIKLSELTENDVIEHCRLRNNAGAGPATVSHDVSYLGSVLDAAKPVYGINYTSNP
                     AKSARPYLLKLGLIGKSNRRNRRPASDELDMLIEGLQQRSTHKCSKIPFVDILKFSVW
                     SCMRIGEVCRLRWEDLDQEQKSILVRDRKDPRKKEGNHMKVALLGEAWDIVQRQPKKS
                     EFIFPYNSTSVTAGFQRVRSKLGIKDLRYHDLRREGASRLFEAGFSIEEVAQVTGHRS
                     LNVLWQVYTELYPKSLHNRFEELQKSRNKTS"
     gene            1549..2586
                     /gene="dusA"
                     /locus_tag="N7C74_003846"
     CDS             1549..2586
                     /gene="dusA"
                     /locus_tag="N7C74_003846"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HGU2433636.1"
                     /translation="MHDNHEAQKINQTSVMPEKTGVYWNSRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     LHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2720..2962
                     /gene="pspG"
                     /locus_tag="N7C74_003847"
     CDS             2720..2962
                     /gene="pspG"
                     /locus_tag="N7C74_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HGU2433637.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(3128..4111)
                     /locus_tag="N7C74_003848"
     CDS             complement(3128..4111)
                     /locus_tag="N7C74_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HGU2433638.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            4194..5609
                     /gene="dnaB"
                     /locus_tag="N7C74_003849"
     CDS             4194..5609
                     /gene="dnaB"
                     /locus_tag="N7C74_003849"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGU2433639.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISSYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5662..6741
                     /gene="alr"
                     /locus_tag="N7C74_003850"
     CDS             5662..6741
                     /gene="alr"
                     /locus_tag="N7C74_003850"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HGU2433640.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKVGEPVGYGGTWISERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6994..8187
                     /gene="tyrB"
                     /locus_tag="N7C74_003851"
     CDS             6994..8187
                     /gene="tyrB"
                     /locus_tag="N7C74_003851"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HGU2433641.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAVPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLAMLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1978 a         2022 c         2136 g         2217 t
ORIGIN      
        1 tcgatagtgc tagtattgaa tgatttccag ccgttaccga tttacgtgtt tattagtgaa
       61 caaaccactc gtcagaagat tcccaggtat ctttcagagt ctcctgaaca aatgtttttg
      121 cagaatcctt atctgcagtg cgtgtaacag aaagcccatc attgctggtg gcttttacga
      181 tcacttctac atcgtcataa cgcttactga tgcgccgagt taattcttcc tttaacgcat
      241 ccacagcacc gtttggcatt ttagtcattt tctctttggc tatgcagatt tcaatacgca
      301 taaaaatccc tctatactgt gtttgtatac agtgttattt ttatctgtat aaataaacag
      361 tgtcaagagg tcttgtttct gctcttttgg agttcttcaa aacgattatg taaagatttc
      421 ggatacagtt cggtatatac ctgccatagc acgtttaatg aacgatgccc tgtaacttga
      481 gcgacttcct caatactaaa accagcctca aataagcgac ttgccccttc tctacgcaaa
      541 tcatgatatc gcagatcttt aatacctaat ttgcttctta ccctctgaaa tcctgcggta
      601 acagaagtgc tgttatatgg aaaaatgaat tctgattttt ttggttgtcg ctggacgata
      661 tcccaggctt ccccaagcaa ggcaactttc atatggttac cttccttttt acgtggatct
      721 ttcctatctc ttactagtat agatttttgt tcttggtcga gatcttccca tcgtaaccgg
      781 catacttctc cgattcgcat acaggaccac acagaaaatt tgaggatatc aacgaacgga
      841 atttttgagc atttatgagt agatcgttgt tgaaggcctt caatgagcat gtcaagttca
      901 tcagatgctg gtctacgatt acgacggttt gatttaccaa tcaaaccgag tttaagtaga
      961 tatggacgag cgcttttcgc cgggtttgat gtgtaattga ttccgtatac aggttttgcc
     1021 gcatccagaa cactgccaag ataactaaca tcgtggctga ctgttgctgg gcctgcacca
     1081 gcgttgtttc ttagcctgca atgttcaatt acgtcatttt ctgtcagttc agatagtttg
     1141 atcgcggata tgtcactatc cataagcagt tccagcacat atcttttagt acggcctgct
     1201 ttacctccgg catttgggtc atttaaatat ttgtgtagta agtcacggac tgtaagtccg
     1261 tcaactgcat ttgatgatgg aatgccatat agatctaatt ccatcacttt ctgtgtgccc
     1321 catgttttgg catgagcatg tttagggaat gttttgcttt ccctgtaagt gataacacct
     1381 ttttctttga taatcacatt acagcgatag cgtggtgtgc catcggattt tagtcgtttc
     1441 tctatgttat agtacgccat tacacgacct cgttatttcg ggttcccata aaacgtggga
     1501 acctgtgcgg gaacctaacg cgagaaaaat agcctgaaat gttcaaaaat gcacgataat
     1561 catgaagcac aaaaaattaa tcaaaccagc gtgatgcctg aaaaaactgg tgtttactgg
     1621 aattctcggt ttagcgttgc accaatgctc gactggacgg acagacattg ccgctatttc
     1681 ttgcgtctgc tttcccgcaa tacgttgctg tataccgaaa tggtgactac aggggcgatt
     1741 attcacggta aaggtgatta cctggcgtac agtgaagaag aacatccggt agcgttgcaa
     1801 ctcggcggta gcgatccggc ggcgctggcg cagtgtgcga agctggcaga agcgcgtgga
     1861 tatgatgaga tcaacctgaa tgtcggctgc ccgtctgacc gggtgcagaa cggcatgttt
     1921 ggtgcgtgtc tgatgggtaa tgcgcagctg gttgccgact gcgtgaaagc gatgcgcgat
     1981 gtggtgtcga ttccggtgac ggtgaaaacg cgtattggca tcgatgacca ggacagctat
     2041 gaatttctct gcgatttcat caacaccgtt tccggcaaag gcgagtgtga gatgttcatc
     2101 atccacgcac gtaaagcctg gctttcgggg ttaagcccga aagaaaaccg tgaaatcccg
     2161 ccgctcgatt atccgcgtgt gtatcaactg aagcgtgact ttctgcatct gacgatgtcg
     2221 attaacggtg gtatcaagtc gctggaagag gctaaagcgc atttgcaaca tatggatggc
     2281 gtgatggtcg ggcgcgaagc gtatcagaat ccgggtattc tggcggcggt agaccgagag
     2341 atctttggtt cctcggatac cgatgccgat ccggtggcgg tagtgcgcgc catgtatccg
     2401 tacattgagc gtgaactcag ccaggggacg tatcttggcc atattacccg gcatatgttg
     2461 ggcttgttcc agggtattcc tggcgcgcgg cagtggcggc gttatttaag tgaaaatgcc
     2521 cataaagcgg gtgcagacat taacgtgctg gaacacgcgc tcaaactggt ggcggataag
     2581 cgttaacttt tcaccaaaaa gtagtcaaat tcaccacgcc ctgcgcaccg tcgcggggcg
     2641 ttttgctgtt aaatcaatag attatttttg gcatgattct tgtaatgcct gcaagagatt
     2701 tcatatttgg gagagcatca tgctggaact actttttgtg attggctttt ttgtcatgtt
     2761 gatggtcacc ggcgtttcgt tgctgggcat tatcgccgcg ctggttgtgg cgacggccat
     2821 tatgttcctc ggcggtatgc tggcattgat gattaagttg ctgccgtggt tactcctggc
     2881 gattgcggtg gtgtgggtta ttaaggcgat taaagcacca aaagtgccga aatatcagcg
     2941 ttatgaccgc tggcgttact aagggattgt gcggatgatc acaacctaag gttttatcct
     3001 tagaacaaaa taggaattga taatcaaatc tgtcactatt gcgcctctaa cagattcatc
     3061 gtgctgtacc ctacatacag ccgaactata aaaagaaagg gcttcccagg tggaagccct
     3121 atttctttta tggaatcaac aggctggaac cttgcgtcgc gcggctttcc agaatctcat
     3181 gcgcacgctg cgcatccttc agcggatatt tctgctgctc ggcgacatcg accttaatca
     3241 caccgctggc aatcaaagag aacagttcat tactggcctc ggttaattcc tcccgcgtgg
     3301 tgatatagcc ttgcagggaa gggcgtgtca catacaacga gcctttctga ttgagaatgc
     3361 ctaagttcac accggtaacc gcacctgatg agttgccaaa actcaccatt aagccgcggc
     3421 gttgcaggca atccagcgac cgttcccagg tgtctctgcc cacggaatcg tacactacgc
     3481 gcactttttt accaccggtg atctctttta accgctcgac cagattctct tcacgatagt
     3541 taataacctg ccacgcgccc gcttttagcg cgctctgcgc tttttgcgcg gttcctacgg
     3601 tgccgataag tttcgcgccc agggcttttg cccactggca ggcaatcaag ccaacgccgc
     3661 cagctgctgc gtggaacagg aactgttcat cgggtttaat ttcataggtt ttgcgcagca
     3721 gataataaac cgttaagcct ttcaggaaag atgctgcagc ttgctcaaaa gaaattgccg
     3781 caggcagaat cgccgcttta tccgcattaa tgttatgcac agagctgtaa gcgcctaacg
     3841 ccgactgcgc atagactaca cgatcgcctg ccttaatatg ctttacacca ctgccgactt
     3901 tactcacgat gcctgccgct tcggtaccta atccgctggg tagtgatggc ggcgggtaaa
     3961 ggccgctgcg gatatacgtg tcgataaaat tgatgccgat ggctttattt tcgacctgga
     4021 tttcattctc cgccggatcg gcaggagtga actctacggc ttgaagtact tccgggccac
     4081 cgtgcttgtg aaattcaatt cgtgttgcca tgtgtcctcc agaacgtatc gtcagggtct
     4141 gcttcatatg gtaaagtttc gacccattct ttatctcggt aactccattc actatggcag
     4201 gaaataaacc cttcaacaaa cagcaggctg aaccccgcga acgcgatcca caagttgccg
     4261 ggctgaaagt gcctccgcac tcgatcgaag cggagcagtc ggtgttgggc ggtttaatgc
     4321 tggataacga acgctgggat gatgtagccg agcgtgtggt agcagacgat ttttacaccc
     4381 gcccacaccg tcatatcttt actgaaatgg cgcgtttgca ggaaagcggt agtcctatcg
     4441 atctgattac ccttgcggaa tcgctggaac gccaggggca actcgatagc gtcggtggtt
     4501 ttgcttatct ggcagagttg tcaaaaaata cgccaagtgc tgcgaacatc agttcctatg
     4561 cggacatcgt gcgtgaacgt gccgttgtcc gtgagatgat ctcggttgcg aatgagattg
     4621 ccgaagctgg ttttgatccg caggggcgta ccagcgaaga tctgctggat ctggctgaat
     4681 cccgcgtctt taaaattgcc gaaagtcgtg cgaacaaaga cgaagggccg aagaacatcg
     4741 ccgatgtgct cgacgcaacc gtggcgcgta ttgagcagtt atttcagcag ccacacgatg
     4801 gcgttaccgg ggtaaacacc ggttatgatg atctcaacaa aaaaaccgct ggcttgcagc
     4861 cgtcggattt gatcatcgtc gccgcgcgtc cgtcgatggg taaaacaaca tttgcgatga
     4921 acctcgtcga aaacgcggcg atgttgcagg ataaaccggt acttattttc tcgctggaga
     4981 tgccatcaga acagatcatg atgcgttctc tggcgtcgct gtcgcgcgtt gaccagacta
     5041 aaatccgtac cgggcagctc gatgatgaag actgggcgcg catttccggc accatgggta
     5101 ttttgctcga aaaacgcaat atctatatcg atgactcctc cggcctgacg ccaacggaag
     5161 tgcgttcccg cgcacgccgt attgcccgtg aacacggcgg catcgggctt atcatgatcg
     5221 actacctgca actgatgcgc gtaccggcac tttccgataa ccgtacgctg gaaattgcag
     5281 aaatctcccg ctcgctgaaa gcactggcga aagaactgaa cgtgccggtg gtggcgctgt
     5341 cccagttgaa ccgttctctg gaacaacgtg ccgacaaacg tccggtcaac tccgacctgc
     5401 gtgaatctgg ctctatcgag caggatgcgg acttgatcat gtttatctat cgtgatgagg
     5461 tgtatcacga aaacagtgat ttaaaaggca tcgcggaaat tattatcggt aaacaacgta
     5521 acggcccaat cgggacggta cgcctgacct ttaacggtca atggtcgcgc ttcgacaact
     5581 atgcggggcc gcagtacgac gacgaataat aattatttta tgaattaggt aattaaagca
     5641 aacacttatc aaggaacaca aatgcaagcg gcaactgttg tgattaaccg ccgcgctctg
     5701 cgacacaacc tgcaacgtct tcgtgaactg gcacctgcca gtaaaatggt tgcggtggtg
     5761 aaagcgaacg cttatggtca cggtcttctt gagaccgcgc gaacgctccc cgatgctgac
     5821 gcctttggcg tagcccgtct cgaagaagct ctgcgactgc gtgcgggggg aatcaccaaa
     5881 cctgtactgt tactcgaagg cttttttgat gccagagatc tgccgacgat ttctgcgcaa
     5941 cattttcata ccgccgtgca taacgaagaa cagctggctg cgctggaaga ggctagcctg
     6001 gacgagccgg ttaccgtctg gatgaaactc gataccggta tgcaccgtct gggcgtaagg
     6061 ccggaacagg ctgaggcgtt ttatcatcgc ctgacccagt gcaaaaacgt tcggcagccg
     6121 gtgaatatcg tcagccattt tgcgcgcgcg gatgaaccaa aatgtggcgc aaccgagaaa
     6181 caactcgcta tctttaatac cttttgcgaa ggcaaaccag gtcaacgttc cattgccgcg
     6241 tcgggtggca ttctgctgtg gccacagtcg cattttgact gggtgcgccc gggcatcatt
     6301 ctttatggcg tctcgccgct ggaagatcgc tccaccggtg ccgattttgg ctgtcagcca
     6361 gtgatgtcac taacctccag cctgattgcc gtgcgtgagc ataaagtcgg agagcctgtc
     6421 ggttatggtg gaacctggat aagcgaacgt gatactcgtc ttggtgtagt tgcgatgggc
     6481 tatggcgatg gttatccgcg cgccgcgccg tccggtacgc cagtgctggt gaacggtcgc
     6541 gaagtgccga ttgtcgggcg cgtggcgatg gatatgatct gcgtagactt aggtccacag
     6601 gcacaggata aagccgggga cccggtcatt ttgtggggtg aaggtttgcc cgtagaacgt
     6661 atcgctgaaa tgacgaaagt aagcgcttac gaacttatta cgcgcctgac ttcaagggtc
     6721 gcgatgaaat acgtggatta atcgttctgt aatatttgat tgtctgtgcc ggatgcggcg
     6781 tgaatgcctt atccggccaa taaaatccta ccaattcaat aagttgatat tctttcatgc
     6841 tcttataaag gtcgtgcctc tggcggatgt acgtttgtca tgagtctcac tctgttgcta
     6901 attgccgttc gcccctgaac atccactcga tcttcgcctt cttccggttt attgtgtttt
     6961 aaccacctgc ccgtaaacct agagaaccat cgcgtgtttc aaaaagttga cgcctacgct
     7021 ggcgacccga ttcttacgct tatggagcgt tttaaagaag accctcgcag cgacaaagtg
     7081 aatttaagta tcggtctgta ctacaacgaa gacggaatta ttccacaatt gaaagccgtg
     7141 gcggaggcgg aagcgcgcct gaatgcggtg cctcatggcg cttcgcttta tttaccgatg
     7201 gaagggctta acagctatcg ccatgccatt gcgccgctgc tgtttggtgc cgaccatccg
     7261 gtactgcaac aacagcgcgt agcaaccatt caaacccttg gcggctcagg ggcattgaaa
     7321 gtgggcgcgg atttcctgaa acgctacttc ccggaatcag gcgtctgggt cagcgatcct
     7381 acctgggaaa accacgtagc aatattcgcc ggggctggat tcgaagtaag cacttacccc
     7441 tggtatgacg aagcgactaa cggcgtgcgc tttaatgacc tgttggcgat gctgaaaaca
     7501 ttacctgccc gcagtattgt gttgctgcat ccatgttgcc acaacccaac gggtgccgat
     7561 ctcactaatg accagtggga tgcggtgatt gaaattctca aagcccgcga gcttatccca
     7621 ttccttgata ttgcctatca aggatttggt gccggtatgg aagaggatgc ctacgccatt
     7681 cgcgccattg ccagcgctgg attacccgct ctggtgagca attcgttctc gaaaattttc
     7741 tccctttacg gcgagcgcgt cggcggactt tctgttctgt gtgaagatgc cgaagctgca
     7801 ggccgcgtac tggggcaatt gaaagcaaca gttcgccgca actactccag cccgccgaat
     7861 tttggtgcgc aggtggtggc tgcagtgctg aatgacgagg cattgaaagc cagctggctg
     7921 gcggaagtag aagagatgcg tactcgcatt ctggcaatgc gtcaggaact ggtgaaggta
     7981 ttaagcacag agatgccaga acgcaatttc gattatctgc ttaatcagcg cggcatgttc
     8041 agttataccg gtttaagtgc cgctcaggtt gaccgactac gtgaagaatt tggtgtctat
     8101 ctcatcgcca gcggtcgcat gtgtgtcgcc gggttaaata cggcaaatgt gcaacgtgtg
     8161 gcaaaggcgt ttgctgcggt gatgtaatgc aggaaagcag gctggaatta cccagcctgc
     8221 agtgaaatta aactgtcgtc gctttcactc tttctttata gatgattttt ttgataccat
     8281 cgttctacgt gagagataat aaacgttgtt agttctttta ttgttaagtt tatcccaatt
     8341 atctggaatt cct
//
LOCUS       DBFIFM010000190         8282 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.190, whole genome shotgun sequence.
ACCESSION   DBFIFM010000190 DBFIFM010000000
VERSION     DBFIFM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.190"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..117
                     /locus_tag="N7C74_003852"
     CDS             <1..117
                     /locus_tag="N7C74_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU2433642.1"
                     /translation="DAADLDIATDEQTSLLAAWKQYRMPLNHVDTSVSPDIE"
     gene            287..1168
                     /gene="nleH1-1"
                     /locus_tag="N7C74_003853"
     CDS             287..1168
                     /gene="nleH1-1"
                     /locus_tag="N7C74_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-1"
                     /protein_id="HGU2433643.1"
                     /translation="MLSPYSVNLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDNGAQV
                     KVGNRTYRVVATDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNASPVSAAM
                     ERGIVHSNRPDLPPVDYAPPELPSVDYNRLSVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTTSQSNEEVTSEVRCFNQYYGAGSAEKIYGNNGDIIGIRMDKINGESLLNISSLPAQ
                     AEHAIYDMFDRLEQKGILFVDTTETNILYDRAKNEFNPIDISSYNVSDRSWSESQIMQ
                     SYHGGKQDLISVVLSKI"
     gene            1216..1337
                     /locus_tag="N7C74_003854"
                     /pseudo
     CDS             1216..1337
                     /locus_tag="N7C74_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            1399..2097
                     /gene="nleD"
                     /locus_tag="N7C74_003855"
     CDS             1399..2097
                     /gene="nleD"
                     /locus_tag="N7C74_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector zinc metalloprotease NleD"
                     /protein_id="HGU2433644.1"
                     /translation="MRPTSLNLVLHQSSRSSSMSDTDIESLVKASSVQWIKNNPQLRF
                     QGTDHNIYQQIEAALDKIGSTETGRVLLNAIESISRLKSETVVIHLNSSRLGVMAHRD
                     IDAENHRGTGSDFHCNLNAVEYPCGEGISVVDFHATIVFHELLHVFHNLNGERLKVES
                     SRAESQKYSPLLLEEARTVGLGAFSEEVLSENKFHEEIGMPRRTSYPHDSALIHDDNT
                     VSLGFQQVRLHPLL"
     gene            complement(2194..2286)
                     /locus_tag="N7C74_003856"
                     /pseudo
     CDS             complement(2194..2286)
                     /locus_tag="N7C74_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504497.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2604..3080)
                     /gene="ybhB"
                     /locus_tag="N7C74_003857"
     CDS             complement(2604..3080)
                     /gene="ybhB"
                     /locus_tag="N7C74_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HGU2433645.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(3139..4428)
                     /gene="bioA"
                     /locus_tag="N7C74_003858"
     CDS             complement(3139..4428)
                     /gene="bioA"
                     /locus_tag="N7C74_003858"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HGU2433646.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQHFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            4515..5555
                     /gene="bioB"
                     /locus_tag="N7C74_003859"
     CDS             4515..5555
                     /gene="bioB"
                     /locus_tag="N7C74_003859"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HGU2433647.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMSYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            5552..6706
                     /gene="bioF"
                     /locus_tag="N7C74_003860"
     CDS             5552..6706
                     /gene="bioF"
                     /locus_tag="N7C74_003860"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HGU2433648.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     TCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPSGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            6693..7448
                     /gene="bioC"
                     /locus_tag="N7C74_003861"
     CDS             6693..7448
                     /gene="bioC"
                     /locus_tag="N7C74_003861"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HGU2433649.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRQSADVLLAMLPQRKYP
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHRIQPITLWFDDALSAMRSLKGIGATHLHEGRDQRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIVRE"
     gene            7441..8118
                     /gene="bioD"
                     /locus_tag="N7C74_003862"
     CDS             7441..8118
                     /gene="bioD"
                     /locus_tag="N7C74_003862"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HGU2433650.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRSIESSVMSSG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
BASE COUNT         2078 a         1983 c         2223 g         1998 t
ORIGIN      
        1 gatgctgcag atttagatat tgcgacggat gagcagacat cgttactggc ggcatggaag
       61 cagtatcgta tgccgctcaa tcatgttgat acgtctgtat ctccagatat cgagtgaccg
      121 gtaatacctg cgttatagtt cgtaaacgtt cgtttgatgg gatgctggaa ggatgaatta
      181 gttgccagat acaaaaagca agagttcatt tctaattttt gttgccatgt taggggggat
      241 gtttgttaag gaaatttaga tgggtttatt ttgaaggttg aaatgtatgt tatcgccata
      301 ttctgtaaat ttgggatgtt catggaattc tttaaccaga aacctgactt cgcctgataa
      361 tcgtgtttta tcctctgtaa gggatgctgc cgttcattct gataatgggg cgcaagtaaa
      421 ggttggcaac agaacatatc gtgttgttgc caccgataat aagttttgcg ttacaagaga
      481 aagtcatagt ggttgtttta ctaatctgtt gcacaggctg ggatggccta agggggagat
      541 tagcaggaaa attgaggtca tgctgaatgc atcaccagtg agcgctgcta tggaaagagg
      601 cattgttcat tcgaacagac ctgatttacc tcctgttgat tatgcaccgc cagagttacc
      661 gagtgtggac tataacaggt tgtcagtacc tggtaatgtt attggcaaag gggggaacgc
      721 tgtagtatat gaagatgctg aggatgcaac aaaagtcctg aagatgttta ctacatctca
      781 aagcaatgaa gaggtgacaa gcgaagttcg ttgcttcaac caatattatg gtgccgggag
      841 tgcagaaaaa atatatggca ataatggtga tattattggt attagaatgg ataaaataaa
      901 tggagaatcg cttttaaata tttcgtcctt gccagcacag gctgagcatg ctatttacga
      961 tatgtttgat agactggagc aaaaaggaat tctttttgtc gatacaacag agacaaatat
     1021 cttatatgac cgcgcgaaga atgagtttaa tccaatagat atatcatctt ataatgtttc
     1081 cgaccgttca tggagtgaaa gtcaaataat gcaatcttat catggcggaa agcaagatct
     1141 tattagtgtg gtattaagta aaatttagta attttatcca gtgtagtgga tttgttgcat
     1201 ggatggagtt ggtaacaaat tgtgtggttg gcaattcttg gcggatcacg gaaatgggca
     1261 atgtcgctga ggactggcaa atggctatga tccgctttat tatcgggttt ggtgaccgcc
     1321 ttgacggtca cttttaaaaa aatcattttc acagagtggt aaacaggctg ctaataagta
     1381 gcattctcag gagtcctgat gcgccctacg tccctcaact tggtattaca tcagtcatca
     1441 aggtcgagct caatgtcaga tacagatatc gagtctcttg taaaagcatc gagcgttcaa
     1501 tggataaaaa ataatccgca acttcgtttc caggggactg atcataatat atatcagcag
     1561 attgaagcag cactcgataa gattggctct acagagacag ggcgtgtact cctgaatgct
     1621 attgaatcaa tatcccgact taaatcagaa acagtggtaa tacacctcaa ctcttccaga
     1681 ctaggagtta tggcacatag agatatagat gctgagaacc atcgggggac tggttccgat
     1741 tttcactgta atctgaatgc agttgaatat ccctgtgggg aggggattag cgtggtggac
     1801 tttcatgcga ctattgtttt tcatgagttg ctccatgttt tccacaattt aaatggggag
     1861 cgtttgaaag ttgagagttc ccgagcagaa tcacaaaaat actctccact tttactcgaa
     1921 gaagccagga ctgttgggtt gggggctttt tcagaggagg tgctttcaga aaataaattc
     1981 cacgaagaga ttgggatgcc ccgtagaacc tcctacccgc acgactcagc tcttattcat
     2041 gatgacaata cagtgagtct gggattccaa caggtaagac tgcatccatt gctttagctg
     2101 tgtttcttgt gctgaatata tattggtggg gcaaacggag gcacacaaaa ctttgcactg
     2161 gattgcaagg ctttgtgcta ttccgatagt ggttcaggtg gagtacccca ccttttcatc
     2221 aagccagccc acccaccact gcatcatttc tctgcgctta tagagatact gagcatggtt
     2281 gtaaattcag caaacgacgt aatgtgtttg acaaaaaatt agcgcaagaa gacaaaaatc
     2341 accttgcgct aatgctctgt tacaggtcac taataccatc taagtagttg attcatagtg
     2401 actggatatg ttgtgttttg tagcattatg cagtctattt tttagactaa atgcattgta
     2461 atacattgat attattgggt tttgctgttt cgcgttcagc ttttttatac taacttgagc
     2521 gaaacgggaa ggtaaaaaga caaaaagttg tttttaatac ctttaagtga taccagatag
     2581 cattgcgcca tctggcagag tgattaacta aacatcgcgg taatcgatgc gcttgccaga
     2641 gagtggaaat gaacgttaaa cccgaccatc gcgccgctgg ctccttcatc gacatcaata
     2701 cgctctacat ccagcgcgtg aacggtaaaa atgtagcgat gggtttcgcc tttcggcggc
     2761 gctgcgccat cgtatccggt tttaccaaaa tcggtacgcg tctgcaaaac gccgtctggc
     2821 attgctacca gaccagagcc aaacccttgc ggtaatacgc gggtatcagc gggtaaatta
     2881 acaactaccc agtgccacca gccggagccg gttggcgcat ccgggtcgta gcaggtgaca
     2941 acaaaacttt tcgttcccgc aggaacatca tcccacgcca gatgcggtga aatattatcg
     3001 ccatcgtaac ccatgccgtt aaagacatga cgatgcggca acttatcgcc atcgcgcaga
     3061 tcgttactga tgagtttcat aaaccctcct ttctagtttg cagaaagtgt agccagaaac
     3121 tctcacgctg acttcccgtt attggcaaaa aaatgtttca tcctgtaccg cgcggttaac
     3181 cgctgcggtc agacgctgca actgttgcgg gagaataata tagggcggca tcaggtaaat
     3241 cagtttgcca aaaggccgga tccagacacc ctgttcgaca aagaattttt gcaatgccgc
     3301 catattcacc ggacgagttg tttcgactac gccaatggcc cccagcacgc gcacatcggc
     3361 aaccatttcg gcatcacggg cgggggcaag ttgctcgcgc agctgtactt caatagccgc
     3421 cacctgttgc tgccagtcgc cagattcgag aatcgccagg ctggcgtttg cagccgcaca
     3481 ggccagcgga ttgcccataa aagttggccc gtgcataaag cagccggctt cgccgttact
     3541 gatggtttct gcaacctcgc gcgtggtgag tgtggcggaa agggtcattg tgccgccggt
     3601 taaggcttta ccgaggcaca aaatgtccgg cgcgatttct gcatgttcac aggcaaacag
     3661 tttgccggta cgaccaaatc cggtggcgat ctcgtcggca atcagcaaga taccttcgcg
     3721 atcgcacatt ttgcggattc gttttaacca ttccggatgg tacatgcgca tcccgcctgc
     3781 gccctggaca atcggttcaa tgatcaccgc tgcgatttca tgacgatgcg ccgccatcag
     3841 gcgggcaaag cccaccatat cgcgctcatc ccattcacca tccatgcggc tttgcggggc
     3901 gggagcaaac aagttttctg gcagatagcc tttccacagg ctgtgcattg agttgtccgg
     3961 atcgcacacc gacatcgcgc caaaggtatc gccatgataa ccattgcgga aggtcagaaa
     4021 atgctggcgc gcttcgcctt tggcttgcca gtactgcaac gccattttca tcgccacttc
     4081 caccgctacg gaaccggagt ccgcgagaaa aacgcattcc agcggttgcg gcgtcattgc
     4141 caccagtttg cggcacagct caatggctgg cgcatgggtg ataccgccaa acatcacatg
     4201 cgacatggca tcaatttgcg acttcatcgc cgcattaagc tgcgggtgat tgtaaccgtg
     4261 gatcgccgcc caccaggacg acataccgtc aaccaggcgt ctgccgtcag acaaaattag
     4321 ctcgcaacct tcggcgctcg ccaccggata aaccggtagc ggggaggtca tggatgtgta
     4381 tgggtgccag atatggcgtt ggtcaaaggc aagatcgtcc gttgtcataa tcgacttgta
     4441 aaccaaattg aaaagattta ggtttacaag tctacaccga attaacaaca aaaaacacgt
     4501 tttggagaag ccccatggct caccgcccac gctggacatt gtcgcaagtc acagaattat
     4561 ttgaaaaacc gttgctggat ctgctgtttg aagcgcagca ggtgcatcgc cagcatttcg
     4621 atcctcgtca ggtgcaggtc agcaccttgc tgtcgattaa gaccggagct tgtccggaag
     4681 attgcaaata ctgcccgcaa agctcgcgct acaaaaccgg gctggaagcc gagcggttga
     4741 tggaagttga acaggtgctg gagtcggcgc gcaaagcgaa agcggcagga tcgacgcgct
     4801 tctgtatggg cgcggcgtgg aagaatcccc acgaacgcga tatgtcatac ctggaacaaa
     4861 tggtgcaggg agtaaaagcg atggggctgg aggcgtgtat gacgctgggt acgttgagtg
     4921 aatctcaggc gcagcgcctt gcgaatgccg ggctggatta ctacaaccat aacctcgaca
     4981 cctcgccgga gttttacggc aatatcatca ccacacgtac ttatcaggaa cgccttgata
     5041 cgctggaaaa agtgcgcgat gccgggatca aagtctgctc aggtggcatt gtgggtttag
     5101 gcgaaacggt aaaagatcgc gccggattat tactgcaact ggcaaacctg ccgacgccgc
     5161 cggaaagcgt gccaatcaac atgctggtga aggtgaaagg tactccactg gcagataacg
     5221 atgatgtcga tgcctttgat tttattcgca ccattgccgt agcgcggatc atgatgccga
     5281 cctcttacgt gcgcctttct gccggacgcg agcagatgaa cgaacagact caggcgatgt
     5341 gctttatggc aggcgcaaac tcgattttct acggttgcaa actgctaacc acgcccaatc
     5401 cggaagaaga taaagacctg caactgtttc gcaaactggg gctaaatccg cagcaaactg
     5461 ccgtgctggc aggggataac gaacaacagc agcgtctgga gcaggcactg atgaccccgg
     5521 acactgacga atattacaac gcggcagcac tatgagctgg caggagaaaa tcaacgcggc
     5581 gctcgatgcg cggcgtgctg ccgatgccct gcgtcgccgt tatccagtgg cgcaaggagc
     5641 cggacgctgg ctggtggcgg acgatcgcca gtatctgaac ttttccagta acgattattt
     5701 aggtttaagc catcatccgc aaattatccg tgcctggaag cagggtgcgg agcaatttgg
     5761 cgtcggtagc ggcggttccg gtcacgtcag cggttatagc gttgcgcatc aggcgctgga
     5821 agaagaactg gccgagtggc tgggctattc gcgggcactg ctatttatct ctggttttgc
     5881 cgctaatcag gcggtcattg ccgcgatgat ggcaaaagag gaccgcattg tcgctgaccg
     5941 gcttagccat gcctcattgc tggaggctgc cagtttaagc ccgtcgcagc ttcgccgttt
     6001 tgttcataac gatgtcactc atctggcgcg actgcttgct tccccctgtc cggggcagca
     6061 actggtggtg acagaaggcg tgttcagcat ggacggcgat agtgcgccac tggcggaaat
     6121 ccagcaggta acgcaacagc acaatggctg gttgatggtc gatgatgccc acggcacggg
     6181 cgttatcggg gagcaggggc gcggcacctg ctggttgcaa aaggtaaaac cagaattgct
     6241 ggtagtgact tttggcaaag gatttggcgt cagcggggct gcggtgcttt gctccagtac
     6301 ggtggcggat tatctgctgc aattcgcccg ccatcttatc tacagcacca gtatgccgcc
     6361 cgctcaggcg caggcattac gtgcgtcact ggcggtcatt cgcagtgatg agggtgatgc
     6421 acggcgcgaa aaactggcgg cactcattac gcgttttcgt gccggagtac aggatttgcc
     6481 gtttacgctt gctgattcat gcagcgccat ccagccattg atcgtcggtg ataacagccg
     6541 tgcgttacaa ctggcagaaa aactgcgcca gcaaggctgc tgggtcacgg cgattcgccc
     6601 gccaaccgta ccctctggta ctgcgcgact gcgcttaacg ctaaccgccg cgcatgaaat
     6661 gcaggatatc gaccgtctgc tggaggtgct gcatggcaac ggttaataaa caagccattg
     6721 cagcggcatt tggtcgggca gccgcgcact atgagcaaca cgcagagtta cagcgccaga
     6781 gtgctgatgt cttactggca atgctcccac agcgtaaata cccccacgta ctggacgcgg
     6841 ggtgtgggcc tggctggatg agccgccact ggcgagagcg tcacgcgcag gtgacggcgt
     6901 tagatctctc gccgccaatg cttgcgcaag cccgccagaa agatgctgca gaccattatc
     6961 tggcgggaga tatcgaatcc ctgccgttag cgactgcgac gttcgatctt gcatggagca
     7021 atctcgcggt gcagtggtgc ggtaatttat caacggcact ccgcgagctg tatcgggtgg
     7081 tgcgccccgg aggcgtggtc gcgtttacca cgctggtgca gggatcgtta cccgaactgc
     7141 atcaggcgtg gcaggcggtg gacgagcgcc cgcatgctaa tcgcttttta ccgccagatg
     7201 aaatcgaaca gtcgctgaac ggcgtgcatt atcaacatcg tattcagccc atcacactat
     7261 ggtttgatga tgcgctcagt gccatgcgtt cgctgaaagg catcggtgcc acgcatcttc
     7321 atgaagggcg cgaccagcga atattaacgc gttcacagtt gcagcgattg caactggcct
     7381 ggccgcaaca acaggggcga tatcctctga cgtatcatct ttttttggga gtgattgttc
     7441 gtgagtaaac gttattttgt caccggaacg gataccgaag tggggaaaac tgtcgccagt
     7501 tgtgcacttt tacaagccgc aaaggcagca ggctatcgga cggcaggtta taaaccggtc
     7561 gcctctggca gcgaaaagac cccggagggt ttacgtaata gtgacgcgct ggcgttacag
     7621 cgcaacagca gcctgcagct ggattacgca acggtaaatc cttacacctt cgcagaaccc
     7681 acctcaccgc acatcatcag cgcgcaagag ggcaggtcga tagaatcatc ggtaatgagc
     7741 tccggattac gcgcgcttga acaacaggct gactgggtgt tagtggaagg cgctggcggc
     7801 tggtttacgc cgctttctga cactttcact tttgcggatt gggtaacaca ggaacaactg
     7861 ccggtaatac tggtggttgg cgtgaaactt ggctgtatta atcacgcgat gttgacggca
     7921 caggcgatac aacacgccgg gctgactctg gcgggttggg tggcgaacga tgtcacgcct
     7981 ccgggaaaac gtcacgctga atatatgacc acgctcaccc gcatgattcc cgcgccgctg
     8041 ctgggagaga tcccctggct tgcagaaaat ccagaaaatg cggcaaccgg aaagtacata
     8101 aaccttgcct tgttgtagcc atcctgtatt tggttaaatt gcgagcgaga tcgcgtcttc
     8161 gattgactgc aatttaacca attaaattct aaaataatca cgaaaaaaat tttacttccg
     8221 catcatgcgg cgaatgtggg aattgcccag gcggcggggg ataggggctg cagacagtta
     8281 tc
//
LOCUS       DBFIFM010000191         8261 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.191, whole genome shotgun sequence.
ACCESSION   DBFIFM010000191 DBFIFM010000000
VERSION     DBFIFM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.191"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(258..333)
                     /locus_tag="N7C74_003863"
     tRNA            complement(258..333)
                     /locus_tag="N7C74_003863"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(298..300),aa:Asn,seq:gtt)
     gene            complement(434..1921)
                     /gene="yeeO"
                     /locus_tag="N7C74_003864"
     CDS             complement(434..1921)
                     /gene="yeeO"
                     /locus_tag="N7C74_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HGU2433651.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     FAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLTTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPAVSE"
     gene            2082..2157
                     /locus_tag="N7C74_003865"
     tRNA            2082..2157
                     /locus_tag="N7C74_003865"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Asn,seq:gtt)
     gene            complement(2195..3145)
                     /gene="cbl"
                     /locus_tag="N7C74_003866"
     CDS             complement(2195..3145)
                     /gene="cbl"
                     /locus_tag="N7C74_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HGU2433652.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLHNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIILSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(3247..4164)
                     /gene="nac"
                     /locus_tag="N7C74_003867"
     CDS             complement(3247..4164)
                     /gene="nac"
                     /locus_tag="N7C74_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HGU2433653.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4490..4565
                     /locus_tag="N7C74_003868"
     tRNA            4490..4565
                     /locus_tag="N7C74_003868"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4523..4525,aa:Asn,seq:gtt)
     gene            complement(4621..5556)
                     /gene="ldtA"
                     /locus_tag="N7C74_003869"
     CDS             complement(4621..5556)
                     /gene="ldtA"
                     /locus_tag="N7C74_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU2433654.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPVTVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5618..6697)
                     /gene="cobT"
                     /locus_tag="N7C74_003870"
     CDS             complement(5618..6697)
                     /gene="cobT"
                     /locus_tag="N7C74_003870"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HGU2433655.1"
                     /translation="MQTLANLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALGV
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVNVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPFLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASKIVLPGNTTSDLNS"
     gene            complement(6709..7452)
                     /gene="cobS"
                     /locus_tag="N7C74_003871"
     CDS             complement(6709..7452)
                     /gene="cobS"
                     /locus_tag="N7C74_003871"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HGU2433656.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLVLMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7449..7994)
                     /gene="cobU"
                     /locus_tag="N7C74_003872"
     CDS             complement(7449..7994)
                     /gene="cobU"
                     /locus_tag="N7C74_003872"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HGU2433657.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(8146..>8261)
                     /locus_tag="N7C74_003873"
     CDS             complement(8146..>8261)
                     /locus_tag="N7C74_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HGU2433658.1"
                     /translation="VPAVALIHYLNEMCQSTETAVNDAFCRTLTRESLAIP"
BASE COUNT         2287 a         2233 c         1786 g         1955 t
ORIGIN      
        1 ataaagacgc gacagcgaca tcccgcataa ggcatttttt ctttatcttt gtacggtact
       61 tcatggaaca gagtttttga ccttgcgaat cgtgatgtct gttgggggca atttactcac
      121 tgaagcgtga gactcgatta agcgcacgaa acacagaaat caaaaaaccc ggtcactttt
      181 tacaaggcaa ccgggtaaaa ataattttta ttttttaact gccctgagac tcataaagat
      241 gcctcaaaac taaaatttgg ctcctctgac tggactcgaa ccagtgacat acggattaac
      301 agtccgccgt tctaccgact gaactacaga ggaatcgtga gaacgaggcg aatattagcg
      361 atgcccaccc acaatgtcaa agcctgtttt ttaaatttga aatcgtttgc tgaaataatc
      421 tgcattttgt cgtttattcc gacacagctg gctttttttc acacttttgc ggttcgggtc
      481 gagggtattt ccacagccaa cgtccagtaa ccattcgcca gtaaaacagc acggcccgca
      541 cagcccagtc ggcaaacatt cccatccaga caccaaccac accccagcca agcatgatgc
      601 ccagcacata accgacgaca acccgacaac cccacatgct caacatagaa acccacatag
      661 cgtaacgggc gtcacgagcc cccttaaatc cagcgggtag cacccatgaa gcggaccaaa
      721 taggcataaa taaagcattt agccaaatca gaatcacaac gacatgttta acctgtggat
      781 cctgggtgta aaacgatgcc ataaccccgg caaagggagc cgttagccag gcgatggccg
      841 ttaatccaag agtggtcagc caaaacacat gccgcaactg aatctctgct tgcgctatct
      901 gccctacccc caaccttcgg cctgtaatga tcgtagaagc agaaccgagc gcacttccgg
      961 gtaagttgat aagagccgca attgaaaacg cgataaaatt tccggcaata acactggtcc
     1021 ccattccggc aacgaacatt tgggttaata accgaccact ggtaaataac actgattcga
     1081 cactcgctgg aataccaatc cccatgactt cccagataat gctaaaattc agcggtttaa
     1141 aatagctctt taacgaaatc cttagcgcag gattaaaacc aatcgccagc acccacaaaa
     1201 ttgcaactgc gccaatataa cgagaaatgg ttaaacccaa cccagccccg acaaatccca
     1261 gtcccggcca ggagaaaagg ccgtaaatca atatgccgct aataataata ttaagaatat
     1321 tcaggctacc gttaatcagt agcggtattt tcgtattccc tgcaccacga agtgccccgc
     1381 taccaattag agtgatggca gctgctggat aactgagtac cgtcagctcc agataagtca
     1441 acgccagtgc tttaacttct gtcgtggcat cacccgcgac gaaatcaata atttgctcgc
     1501 caaaatgatg aataagcgtt gccaacagta cggcaaacaa cgtcatgatc accaatgact
     1561 gccgcgtcgc caccctcgct cgtcgtcgat cccgcttacc gagactaaat gccacaacga
     1621 cagtagtacc aagatcgata gcagcaaaaa aagccataat gaccatattg aagctgtccg
     1681 ccaatcccac gccggccatc gcatcttttc ccagccagct gaccagaaaa gtgctcagaa
     1741 cccccatcaa caggacacag gcattctcca tgaagatagg aacagcaaac ggggttatct
     1801 cgcgccagaa caacactttg tagctcttgc gtttagcgtg ccagcgagtg ccgtgaacaa
     1861 cctggcgtaa agcagaggag atattcaaag ccgaccttaa ttgcagaaag tgaaaccaca
     1921 tttcaaataa tgagggagaa tcagcaaagc tgcaaagatt ttcgccatca aattgtctgc
     1981 aaatgcaaca aactgttgat agaaacggca aacagttggg aaaattaaaa atcgggtttg
     2041 acaaaagatt tttcgccgtt aagatgtgcc tcaacagcga ttcctctgta gttcagtcgg
     2101 tagaacggcg gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaaat
     2161 ttctgaaaag cccgctttta tagcgggctt tttgctatat ctgataatca atttcctctt
     2221 cactgttttc catcacctgc cgcttgatat cctcaactga aagtcctgca ttgcaaagtt
     2281 ccagaaagcg ccagacatag ttacgctgaa gttgtcctcg cttcagtccc aaccagacag
     2341 tattagcatc gaaaagatgc cgcgtatcca ggcggattaa attcttttcc tcttgttcac
     2401 cgctggattg ttcggcaact aatccgatcc caagcccaag agcaacatag gttttaatga
     2461 catcagaatc ctgcgcactt aatataatat ctgccagcaa acctttgcgg gcaaatgcgt
     2521 catcaatacg tgagcgcccc gtaatcccct gtcggtaagt gattaacggc cacttcgcta
     2581 ttgattccag cgtcaatggt gtaatttgcg tcaagggatg atcgtgtgga acaagcaaac
     2641 tatggtgcca acgaaaccac gggaaggcga cgagctgcgg gtcattactc aaacgctcgc
     2701 tggcgatacc aatatcagct tcaccattat gcaacaacgt cgcaatttcc tgtggcgtcc
     2761 cctggattag ctcgagccga acctccggga aaagttcgcg aaaagcttta atgacctctg
     2821 gcaagctata acgtgcctga gtatgcgtcg ttgcaatagt gagaacgcca gacgtatcgt
     2881 tggtaaacag gtctgcaagc cgacgaacat tactggcttc attgagaata cgttctgcaa
     2941 tgaccagtaa tgctttgccc ggttcagtca tgcccagcag tcgcttacct cgtcgaacaa
     3001 atatttcgat gccaagttca tcctccagtt cccgaatatg acggctgacg cctgactgtg
     3061 aggtaaaaag catattcgca acctctgtca ggttgtaatc ctgacgtgca gcttcgcgga
     3121 ttatctttag ttgttggaaa ttcacggtaa actccgggca gttcagatat cccgttattg
     3181 ttaaagtcta acgcccggca taacaaataa taaaaacccg catcttattc catcccgata
     3241 taacgcttag ctcaccaatt gccactgcct tttttccatc actggagaac taatcactga
     3301 cattaacaac tctttcactg cctgtgcctg tggcgataag ttcgctctgg cgggtaaatt
     3361 taatgacaaa gagagactca tggaaggcgt ggtaatgcgt gacatccacc catttactgc
     3421 gccacataac gaacgcgcgg ccgattcggg taatactgca acgcccatgc cgctggcaat
     3481 cgctgcggta agcgtagcaa tagactcaat ttcaccaatg acttttgccg tgagccgccg
     3541 tagggaaaac gcctcatcaa cacgaagtct aacagcgctg taatcactgg ggagaaaaag
     3601 gttcatttgt gcaatagcat taacatcaac gctttgcccc gggcaatctt gagttcctac
     3661 caggaaaaga tcttctttca gcaaagcctg actggataca ccagccacag gggaatgctc
     3721 ataaatcacc gccatatcga gttggtgatt tatcagtttt tcgttaagca ctgcaccgct
     3781 attttcatga agatagataa cgacctccgg gaattcagcg cgaaccgcct gtaataaggg
     3841 catggtgatg gatgacgcag cggttcctgg cgcaaagcca atcgagactt gccccgataa
     3901 tgcctgacca acgttatgca ccgccagttg ggcctgctca cactgacgta aaatggcccg
     3961 cgcatgggta tagagaattt ttccggcgtc tgttggcgta acgccccgtt ttgtgcgaat
     4021 caaaagttgt tgatttaact caccttcgag tgtggcaacc tgctggctga gcgctggttg
     4081 tgcgatatgc aatacttcag cagcctgagt caggctacca atatctacaa tttttacgaa
     4141 gtatttcagg cgtctgaagt tcatgttgcc tccggttttt gagaatcggc ccaagtgccg
     4201 ccattactta caaccagatt gcaagatgct tgccagtttt attttggcgt tgatgtacaa
     4261 gctaaccaac tgtcaaataa gagattatga tagattcgtc attggctctt ttcaccagca
     4321 gtcgcgttcc cctgccctat aaaaggaggg tatgcaccac gatggttcat taccaataag
     4381 attgaaatct catcactttg ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc
     4441 ctttgacatc accatgcact gccattaata tgcgccccgt tcacacgatt cctctgtagt
     4501 tcagtcggta gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg
     4561 agccaaattc aaaaaagcct gctttctagc aggctttttg ctttctaatt accaacgctc
     4621 ttaaaacatc tgtcttgaac cagaactaat ttgcacaggc attcccgatc gacgttgcaa
     4681 cgcagcattt gcgcgattca catcaacttc ttgcccgttg ataaacgccc gcaaagatgg
     4741 ggttaccggc aatggcactt ttcggtcaga ctcatattca gcacgattgc gcgacagcgg
     4801 ctcatgaact tccagccact ttgaaccatc cggttccgtt gtgtatttca ctggctgatc
     4861 aataatttgc acacgagtac ctacaggaac attatcaaac agatatttga tatcgtcatt
     4921 gcgcagacga atacagccct gacttacccg gagcccaata ccaaaattgg cattggtacc
     4981 gtggatggca tatagcctgc caatataaat cgcgtacagc cccatgggat tatcgggccc
     5041 cgcaggaaca aatgcgggca aactctcccc tcgtttcgca tattcgcgcc gggtgttcgg
     5101 cgttggcgtc caggttggtg cttcttgttt acgttcaacg gtagtcaccc agttacgcgg
     5161 ggtttctcgc ccagcctggc cgataccaat aggaaagact tccacagtat tactgtctgg
     5221 tgggtagtaa taaagacgca tctcagcgac gttaaccaca atccctttac gaacagtgac
     5281 gggcaaaatc agttgctgcg gaatggtgag ttgcgaacct gacttcggta agaagacatc
     5341 agcgcccgga ttcgcttcca gcatgttact taacccttgc ccgtattgtg cggcaaaagt
     5401 ctccagcggc tgggtattgt gatcaggaac agttacagta agcgactgcc ccactaaacg
     5461 gctaccctct ggaggtaatg gataagttac cgccaggcta gtatggctgg caaaaagcag
     5521 agcaaatgag caaagaatat ttacacgacg catcatgtcc ctttcctaag tcgcgaaagc
     5581 tatcggttaa gtatagcttt tatcagactt ttcgttttta actgttcaaa tcagaagtcg
     5641 tattccccgg tagaacaatt ttactggcag caagttcgcc catgttgttg tatatcgcac
     5701 aagcagcttc gatgatgggc atcgccagag ctgcgccact cccctcacct aaacgcatat
     5761 ccatattgag aaaaggctcc agccccaaat gcgacagcgc tatacgcgcc cctttttctg
     5821 ccgacaggtg agaaggaatg agatacggtt tgattgcagg agacatctgg caggctgcga
     5881 gcgcagcagc ataagaaaga aatccatcca gcaacacggg taaaccgcag gaagcagcgc
     5941 ctaacatcac gccagccatt ccgaccaaat caaatccacc cacttttgcc agaacattaa
     6001 cgccatcctg cggatttggc tgattcaacg taatcgcccg gcgcacaaca tcaattttat
     6061 tagccagttt atctgtcggc aggtttgcgc caatcccaac cacttcttca ggatcccggc
     6121 cagtgattgt gctgactatt gctgctgccg gtgtcgtgtt tgccatcccc agttcaccta
     6181 caccaaacag cgtgacaccg ttttttgcca gctcccgcgt ataacatatg acgtccaaaa
     6241 gtaacttttc agcctgacgg cgactcattg ccggagctga agcaatattg ccgctacctc
     6301 gtgcgacacg catgttgata agcccgggga taggctcagc agtatcaata ccaacatcaa
     6361 ttacgtggac gttagcgccc gcttgtgctg ccagcacaca cacgccggtt gttccgcggg
     6421 tcatattttc agcctgaatg gctgtcactt cttttgggga aatagcgacc ccttcctccc
     6481 agacgccgtg atcggcacac ataaccagta ccgctttttt gcccacatgc ggtatgccat
     6541 tcaaccccgg cattcccgcc agttgtacgc caagcgcctc cagccttccc aggctaccaa
     6601 caggtttgag taacccgtca atatgccgtt gtgcacgcga catagcggca ggatcgatgg
     6661 cagggatcgt attcagtaaa ttggcaagtg tttgcatgtc acgtctcttt atagcagtgc
     6721 cagcaggaag accagttcac caagctctat ggctgctccc agcgtatcgc cggtttgccc
     6781 gcccagcgta cgtttgagaa gctgaccgag gatgaaaatc gccaccatcg tcaccaccat
     6841 agcagccaca ccatgcatac ccggcaataa tacggcagca aaaatagcgg ctaagccgag
     6901 tgtgacgcag gtttgtcgcc catcaatttt gccaatgaac acattgccaa gcccttcttc
     6961 ccgggcataa cgatgacgat acatcaataa tgcggcagtc ccgcggctga ccgcacatgc
     7021 tgccgccagc gatgcaagga tcggttcgcc acgcagagcc aactcgctta acaccaaaat
     7081 ctttgccagt acaacaaaaa tcaatgccag accgccgtgg gtacctaaac ggctatcacg
     7141 catgatttcc aacatgcgat cgcggctacg tgcagaaaat acgccatcac aggtatcagc
     7201 gaggccgtcc agatggaatc ccccggtcat cagcaccagc acaagtacgc taaacagtgc
     7261 cgccagtggt gcgccacacc atgcctgcag caccatgaag accagcccgc taatcgcgcc
     7321 aagcaataat ccaatcaaag gaaaagtaat aataccgcga gaataatgct cgaaatccag
     7381 tccctgggac cagcgacgcg gtacaggcag acgcgtaata aatgagagca tcgcccaaaa
     7441 taatttactc atttgatttt tactccaata cccgaaacca ccagccatac ttcatttgcc
     7501 gcagcggcca actgctgatt tacccgcccg gcaatatcac gaaaatgtcg tgccagacga
     7561 ctctccggca caatccccat tcccacttcg ttagtcacta atacaacctt tgcggggcaa
     7621 cgttggcagg cagcaatcaa cgactgaatc tcagcattaa tcgcctgttc catcgcctga
     7681 taatcccatt catcagggtc tttatcgccg ccataatcaa acaacagatt agtcaccatt
     7741 gtggtaacgc attcaagcaa cacaacctca ttcgggttaa tgtctgcatg aattaattca
     7801 tcaagatgtt gccagcgctc cactgtgcgc cagtgctccg ggcggccttg ccgatgatgt
     7861 tctatccgtg cagccatctc atcatcaagg atttgcgagg tagcgatata cagaacctgt
     7921 gaagagtccc caataagcgc ctctgcgtgg cgactcttcc cgctccgtgc gccccccgtc
     7981 accaaaatca tcataccgac tcctgattca aatgtatgat ggtattcatt gaacagaaca
     8041 atggatcccc caccgttgct gaccgcaaaa catttacgcg attaatactg cgcgtaatat
     8101 aattaaaaat ccagcattct caatccatta cgaaataaga tggcattacg gaatagccag
     8161 actttctcgt gttaacgtcc gacaaaatgc gtcattgaca gcggtttctg tggattgaca
     8221 catttcattc agataatgaa ttaatgctac tgccggaaca a
//
LOCUS       DBFIFM010000192         8235 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.192, whole genome shotgun sequence.
ACCESSION   DBFIFM010000192 DBFIFM010000000
VERSION     DBFIFM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.192"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            12..5440
                     /locus_tag="N7C74_003874"
                     /pseudo
     CDS             12..5440
                     /locus_tag="N7C74_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
     gene            5556..6620
                     /gene="hipA"
                     /locus_tag="N7C74_003875"
                     /pseudo
     CDS             5556..6620
                     /gene="hipA"
                     /locus_tag="N7C74_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            6891..7160
                     /locus_tag="N7C74_003876"
                     /pseudo
     CDS             6891..7160
                     /locus_tag="N7C74_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7594..8157
                     /gene="fimA"
                     /locus_tag="N7C74_003877"
     CDS             7594..8157
                     /gene="fimA"
                     /locus_tag="N7C74_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGU2433659.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
BASE COUNT         2226 a         1727 c         2257 g         2025 t
ORIGIN      
        1 ggagcgttta tatgaataga atctatcgcg tgatatggaa ttgcactcta caggtatttc
       61 aggcctgctc ggaattaact cgcagggtag gtaaaacatc aacggttaat ttgcgaaagt
      121 cctctggact gacaacgaaa ttcagtagat tgacgctggg tgttttgctg gcactaagcg
      181 gttcagtgtc tggtgcgagt ctggaagttg ataatggtca gattaccaat attgatactg
      241 atgttgctta tgatgcctac ctggttggct ggtatggcac tggagtgctt aatattttgg
      301 ctggcggtaa tgcctcctta accactatta ctaccagcgt cattggcggt aatgaggatt
      361 cggagggtac cgttaatgtt ttgggtggca cctggcgatt gtatgatagc ggaaataatg
      421 caaggccttt aaatgtgggt caatccggaa cggggacgct gaatattaaa cagaagggtc
      481 acgtcgatgg aggctattta agattaggta ctcaagctgc aggcgtcggg acggttaatg
      541 ttgagggaga ggactcggtt ttgacgaccg aattattcga aatagggagt tacggtacgg
      601 gctcattaaa tattacggat aagggttacg tcacgagttc aatagtcgcc attttaggat
      661 atcaagcgaa cagtaatggt aaggttgtcg ttgaaaaggg tggcgagtgg ctaataaaaa
      721 ataatgattc ctcaattgaa tttcaaattg gtaatcaagg aactggggag gcgactattc
      781 gcgagggtgg gctgattacg gctgaaaata cgattatcgg tggcaatgcc accggtgtcg
      841 gaaccctgaa tgtgcaggat caagactctg tcatcacggt acgcagactc tataatggat
      901 atttcggtaa tggcgcagtc aatatttcca ataatggact aattaataac aaagaatatt
      961 cattggtggg cgttcaggac ggttcccacg gtgtcgtcaa cgtgaccgat aaagggcatt
     1021 ggaatttcct cggaacgggc gaagctttcc gctatatcta tatcggtgat gctggcgacg
     1081 gtgaacttaa tgtctcgcgt gaaggtaaag tagattcggg aattatcact gcggggatga
     1141 aagaaacagg cacaggcaac cttactgtta aggataagaa ctccgttatc actaatctcg
     1201 gaactaatct tggttatgac ggccacggcg aaatgaatat cagtaatgag gggcttgttg
     1261 tcagcaacgg aggaagctct ctcggttatg gagaaaccgg cgtcgggaag gttagcatca
     1321 ccacgggggg gatatgggag gtcaataaga atgtctatac caccattggt gttgcgggcg
     1381 tcggaaacct caatattagc gatggcggta agttcgtatc gcaaaatatt acttttttgg
     1441 gcgataaagc aagcggtatc ggcacactga acctgatgga tgcgacatcg tcgttcgata
     1501 ctgtgggtat caatgtcggt aattttggta gcggtatcgt aaatgtcagt aatggtgcca
     1561 cccttaattc aacgggctat ggatttatcg gaggaaatgc ctccggtaag ggaatagtta
     1621 atatttcaac ggatagtctc tggaatttaa agacgtcttc tactaacgcc caattgctac
     1681 aggtcggtgt attaggcacg ggtgaactga atattaccac cggaggtata gttaaagcgc
     1741 gtgatacaca gatagctctc aatgacaaaa gtaagggcga cgtgagggtg gatgggcaga
     1801 actctcttct tgaaacattc aatatgtacg tagggacatc tggtacgggt acgttaaccc
     1861 tgacgaatag cggcacgctg aatgtcgaag gtggagaagt ttacttaggt gtttttgaac
     1921 ctgctgtagg aacgctaaac attggtgcag ctcacggtga ggcggcggca gatgccggat
     1981 ttatcaccaa cgcgacgaaa gtagagtttg gctctggcga aggtgttttt gtctttaatc
     2041 atactaataa cagtgatgcc ggctaccagg tcgatatgct gattacaggt gacgataaag
     2101 acggaaaagt gatccatgat gcaggccata cggtgttcaa tgcagggaat acttatagcg
     2161 gtaaaacgct ggtcaatgac ggcctcctga ccattgcgtc tcatacggca gatggggtaa
     2221 cgggcatggg gtcgagtgaa gtaaccattg caagccccgg tacgctcgac attctcgcat
     2281 caacgaacag tgcaggagat tacacgctga ccaatgcgct caaaggcgat ggcttgatgc
     2341 gagttcagct gtcatcctcc gacaagatgt ttggctttac acatgcaaca gggactgaat
     2401 tcgccggtgt tgcccaactg aaagacagta ccttcactct ggaacgcgac aacaccgctg
     2461 cgcttactca cgcgatgttg cagtctgaca ttgaaaatac cacatcggta aacgtgggag
     2521 agcaatccat tggtggactg gccatgaatg gcggtacgct cattttcgat acggatattc
     2581 ctgctgcgac gcttgcagag ggatatatca gcgtcgatac gctggttgtc ggcgcgagtg
     2641 actacacctg gaaaggccgt aactatcagg taaacgggac gggcgacgtg cttatcggcg
     2701 tgcctaaacc gtggaatgat cctatggcga ataaccctct gacgacgctc aatttgctgg
     2761 aacacgatga taaccatgtc ggcgttcaac tggtgaaggc gcaaacggtt attgggtcgg
     2821 gtggctcatt aacgttacgt gatttacagg gcgacgaggt ggaagcggac aaaacgttac
     2881 acattgcgca aaacggaacg gtggtcgccg agggtgatta tggattccgc ctcacgaccg
     2941 caccaggtga tggtttgtac gttaactatg ggctgaaagc gctgaacatc catggagggc
     3001 aaaagctgac gttagccgaa catggcggag cctatggcgc aacggccgat atgtcggcaa
     3061 aaatcggtgg tgaaggggat ctggcaatca atacggtgcg acaggtttcg ctttccaacg
     3121 gtcagaacga ctatcagggg gcaacctacg ttcagatggg gacattacgt accgatgcgg
     3181 atggcgcgct gggcaacacc cgggaactga acatcagcaa cgcagccatc gtcgatctta
     3241 atggatcgac gcagacggta gagacattca ccgggcagat gggttcgact gttttgttca
     3301 aagaagggtc gctgacggta aataaaggtg ggatcagtca gggtgaactg acaggtggcg
     3361 gaaacctgaa tgttacaggg ggaacgctgg ctgtcgaggg gcttaatgca cgctacaatg
     3421 cgttaaccag cgttagccca aatgcggaag tcagcctcga taatacgcag gggttaggca
     3481 gaggaaatat tgccaatgac ggtctgttaa cgctaaaaaa cgtgactggc gaactgcgta
     3541 atagcataag cgggaagggt atcgtgagcg caaccgccag gacagatgta gagctggatg
     3601 gcgataatag ccgctttgtg gggcaattca acattgatac aggcagcgcg ctcagcgtca
     3661 acgagcagaa aaacctgggt gatgcttccg ttatcaataa tggcctgctc accatctcca
     3721 ctgagcgtag ctgggcgatg acgcacagta tcagcggtag cggtgatttg acaaaactgg
     3781 gtaccgggat cctgactctt aacaacgatt cctcggcgta tcagggtacg acggatatcg
     3841 tgggggggga aattgctttc ggttccgact ctgccattaa tacggcaagt caacacatta
     3901 atatccataa cagcggtgtg atgtcgggaa atgtcaccac tgcaggtgat gtgaacgtta
     3961 tgtctggggg gacactgcgt gtcgctaaaa ccacaatcgg cgaatcggcg gcaacttgga
     4021 gaatggcggc acggttcaaa tgaacagtga aggaggaaaa ccggggaatg tactgaccgt
     4081 taacggcaac tataccggaa acaatggcct gatgacgttc aacgcgacgc tgggcggcga
     4141 taattcaccc accgataaga tgaacgtgaa aggcgatacc caagggaaca ctcgcgttcg
     4201 ggttgataac attggcggcg tcggtgcgca aacggtcaac ggtattgaac tcattgaggt
     4261 tggcggtaat tctgcaggta atttcgcgct gaccaccgga actgtcgaag ctggggctta
     4321 cgtctacacg ctggctaaag ggaaggggaa tgacgagaaa aactggtatc tgaccagtaa
     4381 atgggacggc gtaacgccag cggatacacc cgatcccatc aataatcccc ctgttgtgga
     4441 tccggaaggc ccatcagttt atcgcccgga ggccggaagc tatatcagca acattgccgc
     4501 agccaactcg ctgtttagcc atcgcttaca cgaccgtctg ggtgagccgc aatatacaga
     4561 ttcactgcat tctcaggatt cagcaagcag tatgtggatg cgtcatgtcg gggggcacga
     4621 acgttccagt gccggagacg gccagctaaa tactcaggct aaccgctatg tattgcagct
     4681 aggcggcgat ttggcgcagt ggagtagcaa cgcgcaggat cgctggcatc ttggcgtgat
     4741 ggcaggctac gccaatcagc acagtaatac tcagagtaat cgtgtgggtt ataaatcgga
     4801 tgggcgcatc agcggttaca gcgctgggct gtacgcgacc tggtatcaga acgatgcgaa
     4861 taagaccggc gcttatgttg acagctgggc gctgtataac tggtttgata acagcgtcag
     4921 ttccgataac cgttctgctg acgactatga ttctcgcggt gtgacggcct ctgttgaggg
     4981 tgggtatacc tttgaagcgg gaacatgtag cggcagcgaa gggacgctga atacctggta
     5041 cgtccagcca caggcgcaaa tcacctggat gggtgtgaaa gattctgacc atgcccggaa
     5101 agacggaacg cgcattgaaa cggaaggcga cggaaacgtg caaacgcgac ttggggtgaa
     5161 aacctacctg aatagccatc accagcgtga cgatggtaaa cagcgtgagt tccagcctta
     5221 cattgaagcg aactggatca acaatagcaa agtctacgcc gtgaagatga atggtcaaac
     5281 cgtaagccgt gatggtgcgc gaaatctcgg tgaagtacgt accggggttg aggcgaaagt
     5341 aaataacaac cttagcctgt gggggaatgt cggtgtgcaa ctaggtgata aaggctatag
     5401 cgatactcag ggcatgctgg gagtgaaata tagctggtaa acagtataag ccgcatgtcg
     5461 agatggcatg cggcttaata ttgccaactt caaaaggctt ttaaagcctc ccttttatcc
     5521 gcgatcgcgg atatcgcagc gtttatcccg tacagtttga tttattgtca gaaatagggc
     5581 gagacagcgt tggtgccgtg acgttaatac ccgaagacga aaccgtaacg cgtccgataa
     5641 tggcatggga aaagcttact gaagtcagac ttgaagaagt attaacggct tataaagcag
     5701 atatcccgct aggcatgatt agagaagaaa atgactttcg catctcggtt gctggcgcac
     5761 aggagaagac agcactgctc agaataggca atgactggtg cattccgaaa ggaataacgc
     5821 cgacgacgca catcattaaa ttaccgattg gggaaatcag gcagcccaat gcgacgctcg
     5881 atctcagcca aagcgttgat aatgagtatt actgtctgct gctggcgaaa gaacttgggt
     5941 tgaatgtccc ggacgcagaa atcattaaag cgggaaatgt gcgcgcgtta gcggtcgaac
     6001 gttttgacag gcgttggaat gctgagcgaa cggttttact tcgcttgcca caggaggata
     6061 tgtgtcagac attcggttta ccttcatcgg tgaaatatga atcagatgga ggcccaggca
     6121 tcgcgcggat catggctttt ttgatggggt tcagcgaggc gcttcgcgat cgttatgatt
     6181 ttatgaaatt ccaggtcttc cagtggttga ttggcgcaac ggatggccat gcaaaaaact
     6241 tctccgtatt tattcaggct ggcggcagtt atcgactcac gccattttac gacatcattt
     6301 cagcatttcc ggtccttggc ggtacgggaa tacacatcag cgatctcaaa ctggcaatgg
     6361 ggcttaacgc atccaaaggc aaaaaaacgg caatcgataa aatttatccg cgacattttt
     6421 tggcgacagc aaaggtgctg agattcccgg aagtgcagat gcatgaaatc ctgagtgact
     6481 ttgccagaat gattccggca gcactggata acgtgaagac ttcattaccg acagattttc
     6541 cagagaacgt ggtgacggca gttgaaacca atgtgttgag gttgcacggt cggttaagcc
     6601 gagaatacgg tattaagtaa gagtaagata tggggtttgg tcgttgttaa tgagcaggac
     6661 aatcatgacc gccaacgact agattctccc ttggtttata ttggtgcgca taatgtatat
     6721 tatgttaaat cgtatctgag tgatacgaag tgattttccg gtagtcgtta ttcgttcagg
     6781 attccagaac ttaagcttct cctctttcat aattggcctg tgcatgacgg atataggctg
     6841 gctggtatgc cgatttgccg tttgccgcat cttcccagcg ttgaagactc aagctggcaa
     6901 accgcgtgca aatgagcacg ccctctttcc atcaacactt caggcagctt gcgggaatga
     6961 gtccactccg gtatcagaaa tggctacgtc tcaatgaagt caggcgaacg atgctgaatg
     7021 aacattacga tgtcaccaca gcagcctatg ctgtcggtta cgaaagctta tcccatttcc
     7081 gacgggaata tttgcggatg tttggagagt cacctaagag agatattacc aggttaagaa
     7141 agtctgtcgg tcagctttaa taaaccctgc acttatctgt ttagagcaat tcggtgttag
     7201 ttacaccagg caaacattaa ctatagctaa tgatttatag ccatataaac cattagtagc
     7261 ccttgacctt tcctgacact tcccttgttc gcaataatat tctgtttagt cacggtttat
     7321 aaataacatg cataatgaaa tcgtcatgtt atttagcgac ataaaatgtt tttgcaaata
     7381 ctctcttcaa caagtgcagc aataaacttg ttgtgattag gtgtccttgt gatttaaaga
     7441 tattatcgtt cttcgtgaaa tgtaataacc ctggaaacta tattctcagg gcaacaacca
     7501 atcaagaatg attcttgttt tataagcaat aacagaatga cttaattgtt aactctcttt
     7561 attcagctac ttaaatataa attttggaga attatgaaac tcaaacatgt tggtatgatt
     7621 gtcgtttctg tgttggcgat gtcgtctgct gcggtaagcg cagccgaggg tgatgaatca
     7681 gtaacgacca ctgttaatgg cggtgttatt cattttaaag gtgaagtggt aaatgccgct
     7741 tgtgcgattg attccgaatc aatgaaccaa acggttgagc tgggtcaggt tcgttcttct
     7801 cgcctggcta aagcgggtga cctcagctcc gccgttggct tcaatatcaa gctgaatgat
     7861 tgtgatacca atgtttccag taatgcagct gttgcattcc tgggtactac tgtcaccagt
     7921 aatgacgata cgttagcgct gcagagttca gcggcaggct ctgcccaaaa tgtcggtatt
     7981 caaattttgg accgtacggg tgaggtatta atacttgatg gggccacttt tagtgctaaa
     8041 accgacttga ttgatggcac gaatatacta ccattccagg ctcgttatat tgctctcggg
     8101 cagtccgtag ctggtactgc aaacgcagat gcgaccttca aagttcaata tctataattc
     8161 aatattaagg tggctatacg gcatctgaaa ttaaaacagg gtgtgtgtgg catggctacg
     8221 cacacccgag caatt
//
LOCUS       DBFIFM010000193         8220 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.193, whole genome shotgun sequence.
ACCESSION   DBFIFM010000193 DBFIFM010000000
VERSION     DBFIFM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.193"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(88..369)
                     /locus_tag="N7C74_003878"
     CDS             complement(88..369)
                     /locus_tag="N7C74_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2574 family protein"
                     /protein_id="HGU2433660.1"
                     /translation="MNKYWLSGAVFLAYGLASPAFSSDTTTLTINGRISSPTCSMDVV
                     NNHLQQRCGQLLQRVETNYRASSTAKGVTTEVVAVGNNDKRKIVLNRYD"
     gene            complement(631..1740)
                     /gene="apbC"
                     /locus_tag="N7C74_003879"
     CDS             complement(631..1740)
                     /gene="apbC"
                     /locus_tag="N7C74_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HGU2433661.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1872..3905
                     /gene="metG"
                     /locus_tag="N7C74_003880"
     CDS             1872..3905
                     /gene="metG"
                     /locus_tag="N7C74_003880"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HGU2433662.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYTRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4046..7855
                     /locus_tag="N7C74_003881"
                     /pseudo
     CDS             4046..7855
                     /locus_tag="N7C74_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7868..>8220
                     /locus_tag="N7C74_003882"
     CDS             7868..>8220
                     /locus_tag="N7C74_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HGU2433663.1"
                     /translation="MKTYIYQDEKSHKFWAVEQQGNELHINWGKVGTNGQSQVKSFAD
                     TAAAEKAALKLIAEKVKKGYVEQVDANAHSHDASAKKVLVTEEKASINRQAATNGLPW
                     LADNDPIILPPNIAR"
BASE COUNT         2187 a         2057 c         1993 g         1983 t
ORIGIN      
        1 taacatttat gggatgaata aaaaagccaa aatcattcat aggaatgatt ttggcttttt
       61 aaatatgctt aaaacggtgt tggcaaatta atcgtagcga tttaacacga ttttgcgttt
      121 gtcgttatta cccacagcta ccacttctgt cgtaacgcct tttgcagtag aagaagcacg
      181 atagttggtt tcaactcgtt gtaacaactg cccacaacgt tgctgaagat gattattaac
      241 aacatccata ctgcatgtag gggaactaat tctgccattg atggttaatg ttgtagtatc
      301 agaagagaac gcgggtgatg ctagtccata agcaagaaaa acagctcctg acaaccaata
      361 cttattcatt gaacgttatc cctgtagtaa aggttatgcc gggtagattg agtgcataac
      421 acactatagc tgtacatccg ctacacagcc acgagggatg ataaagagac agtggttgca
      481 caatcaatcg cctttgtaca gtttcggact aagatccact gaatattcgc agtattaaat
      541 aagtgttcat taatgcggca aaaacaaata caccgcatca atataacatc tctgcggtgt
      601 atgaagaata accagaatgg ttaataggcg ttagaccgcg cggaaggaaa tctcgcctgg
      661 aatgacttca ccctgccagt agagctgagc tgcaacgcgg tcagccagct ggcggtagat
      721 agcggtaaat tcgctctccg gacggctgat aaccgttggc gtgcctttat cgagatcctc
      781 acgtaaggag atgtgtaacg gcatctgccc cagcagctgt gtatggtatt tttcagccag
      841 tttctctgca ccaccggtac cgaaaatcgg ttcatggtga ccacagttac tgcaaatatg
      901 cacactcatg ttctcgacaa taccgagcac cggaacctcg actttctcga acatcacaat
      961 gccttttttc gcatcgatca acgcgatgtc ctgcggggtg gtgaccacga ctgcaccagt
     1021 tactggaatg ttttgcgcca gcgtcaattg aatgtcaccg gtgcctggcg gcatatcaag
     1081 caccagatag tcgagatccg gccacaaggt ttcctgcaac atctgcatta acgccttgct
     1141 cgccatcggt ccacgccaca ccatcgcatt gtcgtcagtg accagataac caattgaatt
     1201 ggttgccagg ccatgagaca taatcggtgc catatgggta ccgtccggcg aagttggacg
     1261 ctgattttct gcgcccagca tggtgggaat tgatgggccg tagatatcgg cgtccagaat
     1321 gccgactttc gccccttccg ccgccagtgc cagcgccagg tttaccgccg tggaggattt
     1381 acccacgccg cctttaccgg aactgaccgc aatgatgttc ttcacgccat taatacccgg
     1441 ctgattcttc acgcgtttta aggtggcgat attgtgcgac agtttccagt cgatagcttt
     1501 cgccccggtg atacgcagca gttcggcgct acattgctct tttaactctt caaacgcgct
     1561 atgccagacg aatggcataa ccagttccac atgcaacgtg tcgtccatcc aggcgacatg
     1621 gtgcaacgct ttcagcgtgg tgaggttatg cttcagggtt gggtgctgaa aattggccag
     1681 tgtcccggcg accattgcgc gcaaggcttc tggtgatttg gcctgggatt gttcgttcat
     1741 cccgactcct tttttgtata gataaaccat cagctgatag tttacctgaa gaatatagag
     1801 aagtacttac ttaacatttt cccatttggt actatctaac cccttttcac tattaagaag
     1861 taatgcctac tatgactcaa gtcgcgaaga aaattctggt gacgtgcgca ctgccgtacg
     1921 ctaacggctc aatccacctc ggccatatgc tggagcacat ccaggctgat gtctgggtcc
     1981 gttaccagcg aatgcgcggc cacgaggtca acttcatctg cgccgacgat gcccacggta
     2041 caccgatcat gctgaaagct cagcagcttg gtatcacccc ggagcagatg attggcgaaa
     2101 tgagtcagga gcatcagact gatttcgcag gctttaacat cagctatgac aactatcact
     2161 cgacgcacag cgaagagaac cgccagttgt ctgagcttat ctatactcgc ttgaaagaaa
     2221 acggttttat taaaaaccgc accatctctc agctgtacga tccggaaaaa ggcatgttcc
     2281 tgccggaccg ttttgtgaaa ggcacctgcc cgaaatgtaa atccccggat caatacggcg
     2341 ataactgcga agtctgcggc gcgacctaca gcccgactga actgatcgag ccgaaatcgg
     2401 tggtttctgg cgctaccccg gtaatgcgtg attctgaaca cttcttcttt gatctgccct
     2461 ctttcagcga aatgttgcag gcatggaccc gcagcggtgc gttgcaggag caggtggcaa
     2521 ataaaatgca ggagtggttt gaatccggtc tgcaacagtg ggatatctcc cgcgacgcgc
     2581 cgtacttcgg ttttgaaatt ccgaacgcgc cgggtaaata tttctacgtc tggctggatg
     2641 cgccgattgg ctatatgggt tctttcaaga atctgtgcga caagcgcggc gacagcgtaa
     2701 gcttcgatga atactggaag aaagactcca ccgccgagct gtaccacttc atcggtaaag
     2761 atattgttta cttccacagt ctgttctggc cagcaatgct ggaaggcagc aacttccgca
     2821 agccgaccaa cctgtttgtt catggctatg tgacggtgaa cggcgcgaag atgtctaagt
     2881 ctcgcggtac ctttattaaa gccagcacct ggctgaatca ttttgacgcc gacagcctgc
     2941 gttactacta cactgcgaaa ctctcttcgc gcattgatga tatcgatctc aacctggaag
     3001 atttcgttca gcgcgtgaat gccgatatcg ttaacaaagt ggttaacctg gcctctcgta
     3061 atgcgggctt tatcaacaag cgttttgacg gcgtgctggc aagcgaactg gctgacccgc
     3121 agttgtacaa aaccttcact gatgccgctg aagtgattgg tgaagcgtgg gaaagccgcg
     3181 aatttggtaa agcagtgcgc gaaatcatgg cgctggctga tctggctaac cgctatgtcg
     3241 atgaacaggc tccgtgggtg gtggcaaaac aggaaggccg cgatgctgac ctgcaggcga
     3301 tttgctcaat gggcatcaac ctgttccgcg tgctgatgac ttacctgaag ccggtactgc
     3361 cgaaactgac cgagcgcgca gaagcatttc tcaatacaga actgacctgg gatggtatcc
     3421 agcaaccgct gctgggccac aaagtgaatc cgttcaaggc gctgtataac cgcatcgata
     3481 tgaagcaggt tgaagcactg gtggaagcgt ctaaagaaga agtgaaagca gctgccgcgc
     3541 cagttactgg cccactggca gatgatccga ttcaggaaac catcaccttt gacgacttcg
     3601 ccaaagttga cctgcgcgtg gcgctgattg aaaacgcaga gtttgttgaa ggttctgaca
     3661 aactgctacg cctgacgctg gatctcggcg gtgaaaaacg caatgtcttc tccggcattc
     3721 gttctgctta cccagatccg caggcactga ttggtcgtca caccattatg gtggctaacc
     3781 tggcaccacg taaaatgcgc ttcggtatct ctgaaggcat ggtgatggct gccggtcctg
     3841 gcgggaaaga tattttcctg ctaagcccgg atgccggtgc taaaccgggt catcaggtga
     3901 aataatcccc cttcaaggcg ctgcatcgac agcgcctttt ctttataaat tcctaaagtt
     3961 gttttcttac gattttgtct ctctctaacc cgtataaata ctggtagcat ctgcattcaa
     4021 ctggataaaa ttacagggat gcagaatgag acactttatc tatcaggacg aaaaatcaca
     4081 taaattctgg gcggttgagc agcagggaaa cgagctgcat atcaactggg gcaaagtcgg
     4141 cactaacggg caaagccaga taaaaagttt tgcggatgtt gcagcagcgg aaaaagcgaa
     4201 actgaagctt atcgcggaga aaacgaaaaa aggctatgtg gaaaatgctt cagcaaacgt
     4261 gcatatcccc cccatcacca aagccactcc tgaagttgaa acttcccctg agagtaaaaa
     4321 ccaacgcccg tggctggctg atgatgcagt tattccggta actgacgata ttaaccgatt
     4381 tgcttttcct catcgctctc gccccagaga gatcaattat ctgcgcaaag acggtgagat
     4441 atggaagcgt attgcagata acaccagggc atatgatccc gacaacaatt accgctctta
     4501 tcctgaaaac tggcagcagg cttttgctga gttacaaagg cgaattctgg ataatcaaca
     4561 gacagggagt gcgcaatctg atgcagcatt gctctggagt ttttggaact cttactctgc
     4621 agatgaactg gtcgatgact tagtcattcg ctgtggtctg gaaagcgccg ttgaaatcgt
     4681 cctttttgcc cttcaactca aatataaacc agtaaaaggc gcagtaacca ccatcattcc
     4741 acctaatcac gaagcagaat cgctacctag ttagcatcaa cgtctatgtc atcatctttc
     4801 actcgcttca gaagatgagt ggcaacgctg tgtagataaa gtactcaccg ccattccgac
     4861 gctatccccc gcacggcaac cttttgcggc gttactgctc cctgaacgcc cggatatcgc
     4921 ccatgctata gatctacgct atgcagacca aaacgtcccg gcgatgacct ggttaagact
     4981 ggtgataacc gatgatactg ctctgagtac agtggaaaaa tacgacttcc caccgttgta
     5041 tcgtgatttt cgtaattttc gcgcttatct ggcaatgttg ttggcaaata atggggtgcg
     5101 cggtgtaagc cggatacttc ttgagtttac ggaagaccat tctgacaatc ccacttatct
     5161 cttcgaacgc atcagcgaga ctgaaaatct tgtcaaatgg ctatggaaaa cgaatcaccc
     5221 ggatgcgatt caaattctga tcctcggtgt aattggtaag aaaaagcacc tggaatactt
     5281 aagcaaggcc agtcaaaagc atcccgctgc ggctattgcg gcttatgcta ctttgctggc
     5341 aatacatgaa gataaagagt ggcgtaaagc gcttgtcaaa ctgattaccg ccacgccaga
     5401 gttagtatgc gacgtcattc cctgggttaa tgctaaagcg gcaggcatct tatctgaatg
     5461 tcgtccgcag tcagtcgctg aagaatgtga atatgccact gtggatatgt tgccggagct
     5521 gcttgtttcg ccgccctgga tgaccaaaga gaaaaagaaa aatacgcctg tgtttgatct
     5581 tccagtgctt ccagttcctt ctgtttctga tgtcactcca gagattacca aaaaactgac
     5641 ccgtacttac cttgtcaccc acttccagca aatagcccaa cagcaggcga caaaacaaac
     5701 attgtttacc gacctgccgc cgatcaaaaa agcaagctgg gagaaacact taataccgct
     5761 tacaccggag caacaaatac tgtggcatct tggttttgaa aaatggcggg agagcggaga
     5821 aaaaatatac gaaaaaattc cggcgccaca aagcgccgtt gatgccctgc tacgcttcga
     5881 ttttccagca ctcaatgctg aatttgtgca ttatcataac aatgcataca aaagctggaa
     5941 tctcattgcc ctctgttacc tgcctggtca acaggccatt tcttttctta accagatagt
     6001 caaagaagat aactactctg gtgagggaaa tattctggca atttttggta gcgccgccat
     6061 acctgcattt atggcctgcc tgcaacgtga tccacgacgt ctgtgcttct tccccttttt
     6121 cctcggtgtc agtgaactgg ccttaccgat ggcgcaacaa ttacagaaaa aaatgtctta
     6181 tgaagatgcg cgaaactggc taactgatta tccacgccat gccgccgccg ggctgcttcc
     6241 tgtggccctg ggcaaaaaag gaaaagatcg tgattgtgcc cgccaggcgc ttcgtctgct
     6301 ggtgaattta aaccagcgag aaacgattga ggaaatcgcg caaggatata atcaacctga
     6361 tgttttagct gcgctggcaa cattgttcga tagcgaccca ctggaagaat atcccgctaa
     6421 aatcgcccca ctgcccggct tttatcagtt caccttgtgg cgcagaccac ggcttaaaag
     6481 taacaacctg cctctgtcag atgacgctat gcgccacctc ggcactatgc tgagctttcc
     6541 tcgcgacatt accgcctacg ctgggctgga tatcattaga gaaatcttca cccgcgaatc
     6601 actggctgaa tttggctggg atctgtatcc cgcctggaca gaagctggcg cacccgcaaa
     6661 agaaaactgg gcatttactt cgcttgggat tttaggcaat gacgacaccg cgcgtaaatt
     6721 aaccccgctt atccgcgcct ggcctgggga atcccagcat aaacgggcgg tgtccgggct
     6781 ggatgtatta gctgatattg gcagcgatgt tgcgctaatg ctgcttaatg gcatcgcgcg
     6841 aaaaattaaa ttcaaagcat tacaggaaca tgcccgcgag aaaatcaaca tagttgctga
     6901 aaaccgtggg ctgactatgg ctgaactgga agaccgcctg gctccagatt tagggcttga
     6961 tagcagtggc tcgctgatac tggatttcgg cccccgcaaa ttcaccgttg gttttgacga
     7021 aaccttaaaa cctgtagtgt gcgatgcaaa cggcaaagtc ctgaaagatt tacctaagcc
     7081 aaaccagagc gatgaaaaaa ctcaggcaac tgacgcggtt aatctcttca aacagttgaa
     7141 aaaagatgta cgcgccatag ccagccagca gattgatcgt ctggaacagg ctatgtgcca
     7201 gcgcagacgc tggacggcag agcagttccg cctgtttctg gtggagcatc cgctggtacg
     7261 tcacttaacc cgacggctgt tatggggggt ttataacgat gaaaacgccc ttatcacctg
     7321 ttttcgcgtg gcagaagaca gcacttacag cgatgcgcag gatgagttat tcacgctgcc
     7381 agcaggaaac atcggtattc cgcatgtgct ggaaatatcc cctgaatccg ccgctgcatt
     7441 caggcaaatt tacgctgatt acgaactgct tccccctttc caacagctcg agcgaggtag
     7501 ctatcacctt gctgataatg aacgtaatac tcacgaactg acacgctggc aaggacggct
     7561 ttgccaggcc ggacgcattg tcgggctgga acgcagaggc tggcaacgtc tggaagaaag
     7621 cggcagcgtt tatgcgatgc gcaaaacaac ccctcatggc gatctcgaac ttgaaacgga
     7681 acctttttca ttaatttatg gtgaaacggg gtatggcgac cagcacccgg ttgaaagcgt
     7741 aaaaatcacc tcgccagatg atcgctacgg taaacaatcc tcactcactt tctccatgct
     7801 cgacgacatc accgccagcg agctgattaa cgacattgaa tcactgtttg attaaaagga
     7861 tttaacaatg aaaacctata tttatcagga cgaaaaatca cataaattct gggcggtgga
     7921 gcagcaggga aacgagctgc atattaactg gggtaaagtc ggtaccaacg ggcaaagtca
     7981 ggtaaaaagc tttgcagata ctgcggcggc ggaaaaagca gcactgaagc tgattgcgga
     8041 gaaggtgaag aaggggtatg tggagcaagt agacgctaac gctcactcac atgatgcctc
     8101 agcaaagaaa gtcctggtca cagaggaaaa agcctcaata aatcgccagg cagcaactaa
     8161 tggtttaccc tggctggcag acaacgatcc cattattctt cccccgaata ttgcccgcca
//
LOCUS       DBFIFM010000194         8207 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.194, whole genome shotgun sequence.
ACCESSION   DBFIFM010000194 DBFIFM010000000
VERSION     DBFIFM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.194"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            32..400
                     /gene="acpS"
                     /locus_tag="N7C74_003883"
     CDS             32..400
                     /gene="acpS"
                     /locus_tag="N7C74_003883"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HGU2433664.1"
                     /translation="MRIGTDIVEIARITKAIINGEMAFYERVYTSHERLLINPAEPDY
                     ERASGFWAAKEALVKAIGCGFRDGVRFQDIEIKHDAAKAPYFVISGRLQEIFIGQGLQ
                     NNSLSISHCRTHAIATVIVY"
     gene            414..1175
                     /locus_tag="N7C74_003884"
     CDS             414..1175
                     /locus_tag="N7C74_003884"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGU2433665.1"
                     /translation="MNMYKYNDLNEKTVLVTGASGDIGLGICEKYLEQNCDVYALYKS
                     NDVQLTALKASHPAGDKLHIVQCDLACPQSVSALCEQIERQAGKIDVLVNNAGIVKDS
                     LFASMSYEDFTQVIETNMFSIFRLTKDALMLLRAAENPAIINVASIAALIPSVGQANY
                     SASKGAILGFTRTLAAEMAPWGVRVNAVAPGMIESKMVKKVSRAVVRAVTSTIPLRRL
                     GKCEEVANTIVFLSSSASSYIVGQTIVIDGGLVMR"
     gene            1179..1727
                     /locus_tag="N7C74_003885"
     CDS             1179..1727
                     /locus_tag="N7C74_003885"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HGU2433666.1"
                     /translation="MSKYTIPSKIFLEMGGWRQPLLMVDKIADYKYGENGFVSVVKHV
                     TYNEPYLLGHFPEDPIMPGVIISEIFGQASEYLSFLTDICDIWRERFEEELKSLRDIH
                     ARIHRPEMLEIIRTRRSQVRGVLAAQNLKFKDIAYPGDSIDVVSKLAFSDASGFKHYS
                     VTAYVGKKLISQGTIINFRETK"
     gene            1749..2030
                     /locus_tag="N7C74_003886"
     CDS             1749..2030
                     /locus_tag="N7C74_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309314.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGU2433667.1"
                     /translation="MSTMNDAIEQRKEVLMTIKTEIIQRLNIQRDEAQIDDDTALFGN
                     GFKLDSVDATEIVVLLDKVFDIKISESDDPSYMRSINSLATFVIQKKND"
     gene            2066..3226
                     /locus_tag="N7C74_003887"
     CDS             2066..3226
                     /locus_tag="N7C74_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HGU2433668.1"
                     /translation="MKSLAEFHLKNNAVMGVYNNRTLPSSYHDAMTEYKAVRENALLV
                     DYSHLSIVSVMGDDAWALINQLVSADVSIIRDEQAIYSLVLNEEGTIRGDVYVLCSID
                     GYYLLSEDISAAELIASMNTILEKAEELDIQSMPEIQDMRENNWGAILLEGPYSWEIM
                     SEIHGFDVIGLPYYEYMNTEEDLLLFRCGKHGEYAYMTIGEQAKLAEQWEKLLTVGEK
                     YLMQTGGLDYQKIVRLENPCWDASLWEGQAVNPVQLQMQWAVQYDKDDFIGKDAVTEL
                     SQEYTGNKLIGMIAQEECEGIEAGDRVLVEGQDVGYVVKALFSPALQRFIALTLLEKD
                     YAWSDISGYEIQTAHGIIPAQSKCMPFIYNLSMLVSPTEHSYIDASKNKSAA"
     gene            3300..5858
                     /locus_tag="N7C74_003888"
     CDS             3300..5858
                     /locus_tag="N7C74_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HGU2433669.1"
                     /translation="MKTNRKRVVITGLGILSSLAENIQDFREALLNKKNGVKDSERFA
                     KWFENARAAEVLHDIEYSDLNEDVIDSLDNAALWAYKVGKDALSQAGLMDSRDTLKET
                     GMIVGVSSAGTEAFLPLFEQRMEDFSLRKALFSGAFSSCCSSVSTLLGLQGGVELVAT
                     ACTASPNAVGMAFDYIQNGKSKTMLAVGTEPIYLPTFAGFYALNVMHPDTCTPFSGQS
                     GMSIGEGAGAIVLEEYEHAVARGATIYGEILSYATSCDAFHETGPDPRASGAVQVMNK
                     AMANAGVTPEQIDYVNAHGTGTEANDRIETLAMKKVFANHDKLLVSSTKSYFGHNIGA
                     AGIVELIACLATLPDNRVLPTLNFSHARPNCDLDYVPNEFRDRKVNIFMKNNYAFGGN
                     NCCMILSMEPASIPVSTYDSKRVAISGVGAVSAIGHTLNQVLENIWAQDHHVELGSVT
                     FPDDTLEEAKELLAVLDESNQFAELFDEEYSFSEATLPETESNFKTFQVSGLEPRKHL
                     RRFDQRKATRGGTFALIALTEALEQAKRKIKRDGDELGMIMGMSRGPQETTYKYLQSL
                     KPDPRKVRTSEFPGSLMNAIPTFCGISEGIKGYTTTLATGENSALGALTYGYEIVRQN
                     LQPQVLVGGADEYFPSMSLYMDAVTQKIHMTAEASDYQIYGKDPQGYVPGEGACMLLL
                     EDPQLAVARGAEVLAEVVGYGKSCSNTYFDASLLEEKSASMALAIERALTHAGISAGD
                     IDLVCGTSNGSDDHSRIEIDAIYNTFAAQNPAVPVVNYNACFGFVASAAGLLNLAVIT
                     DCIKKQAVPAIPNTVEFFDERVNFVRQSMKLALNHVLLVGATEGGNYYAFVIKG"
     gene            5863..7086
                     /locus_tag="N7C74_003889"
     CDS             5863..7086
                     /locus_tag="N7C74_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HGU2433670.1"
                     /translation="MDNAAIISGYSVWMPYAEDCSQLIDNLKQGKRVARTPWFTSNEE
                     AIKCGFKGNPSVATLKQVDDSALDLLSQLIDEALEQAMLDKHCLAGRNVRVYLTGIGP
                     RIDGLDYKSFYNYNDVEDINLTQSITNLHASKMSQDTISSHLARKYRLQYLPPNMNCT
                     SNSSLTAVHLATQGIEQGGIDLAIVLNCSKIKTQDIWFLETQSMLDSEQVQPFGENSK
                     GVAFAEGFSALLLESAHHRRARQQSEGVRVQTTYTQISAGRSNDASWLSTNVHKVMQA
                     AMKQAEIALDDLAAILPHGNGSAVSDNAEAKAIAMFAGERQIPVLAYKGQIGYTATGS
                     GVVDLIIGHHSLTHHQLIAPVGNDVIIDSMASLVLTDGSVTNHSKRHLLKVGVGVDGS
                     VIGVVMTNMQAGRAK"
     gene            7083..8036
                     /locus_tag="N7C74_003890"
     CDS             7083..8036
                     /locus_tag="N7C74_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU2433671.1"
                     /translation="MTEKSIYLSSWALTEPDDIFYFYQPTWLNAWEAHMYERGLYGTR
                     ANGWGLKRFGVENDIYAPERIRNPQYMPYLEAMEQVTDSALQQMPTKERMKLMKSRTA
                     FIYADSWGESGSFEKISSALHIATIDTLPKNLLKKFSITEPTCKIRGEKQAFVQALRM
                     AQDYLAWDVFDNVVICAAYRAIPVLVFSEEDVGKPPKRLFPRRADEVNLSVERVGCFI
                     LSQRESAIRLQCGEYHSGTAPQHATDIDVMAFSERQQMPSRPAAGVIDLVAHYGESGC
                     LTPALSMDYLTRHIQPGGKMRTVIADKQFGYHYFDLEYLEG"
     gene            8047..>8207
                     /locus_tag="N7C74_003891"
     CDS             8047..>8207
                     /locus_tag="N7C74_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing system ATP-binding protein
                     LolD"
                     /protein_id="HGU2433672.1"
                     /translation="MQSTIKSLPAISLSNIYKCYGAGAGKVDALKSINLDINKGEFLA
                     LCGPSGSGK"
BASE COUNT         2099 a         1926 c         2194 g         1988 t
ORIGIN      
        1 ttcgtaccaa atgtaaagga taacgagact catgagaata gggacagata ttgttgaaat
       61 tgccagaata accaaagcca ttattaatgg agagatggct ttttatgagc gtgtttatac
      121 ttctcatgag cgcttactca ttaaccctgc agaacctgat tatgaacggg cctctggatt
      181 ctgggcggcg aaagaagcgt tagttaaagc gattggttgt ggttttcgtg atggcgtacg
      241 tttccaggat attgaaataa agcatgatgc agccaaagca ccttatttcg ttatttccgg
      301 aagactacaa gaaattttta tcgggcaggg gttgcaaaat aactccctga gtatttcgca
      361 ttgccgtacc cacgccattg cgactgtcat cgtttattaa ctcaacatat gtgatgaata
      421 tgtacaaata taatgattta aatgaaaaaa ccgtactggt gacaggagcc agtggtgata
      481 ttggactggg tatttgcgaa aaatatctgg agcagaactg cgatgtttat gcgctctata
      541 aatccaacga cgtgcaatta acggcgctga aagcaagcca tcctgcaggc gataaactgc
      601 atattgtgca gtgcgatctt gcctgccccc agagcgtgag cgcattgtgc gaacagatag
      661 agcgccaagc tggcaagatt gatgtgctgg taaataacgc gggcatcgtt aaagacagcc
      721 tgtttgcctc gatgagttat gaggatttca cccaggttat cgaaaccaac atgttcagca
      781 ttttccgtct gacgaaagac gcgctgatgc tgcttcgtgc ggcagaaaat cccgccatta
      841 ttaacgttgc ctctattgca gcgctgatcc caagcgtcgg tcaggcgaat tacagcgcgt
      901 caaaaggggc gatcctaggc ttcacccgca cgctggcagc agaaatggca ccctggggcg
      961 tgcgcgtcaa cgcggtcgca ccgggaatga ttgaatcgaa aatggtgaaa aaagtatccc
     1021 gcgcagtggt ccgtgcggta accagtacca tcccgttacg ccgtctgggt aaatgtgaag
     1081 aagtggcgaa caccatcgtc ttcttaagtt cctctgcctc cagctacatt gtcggccaga
     1141 ccatcgttat cgatggcggc ctggtcatgc ggtgatttat gtctaaatac acgattcctt
     1201 cgaaaatctt cctcgaaatg ggcggctggc gtcagccgct gctgatggtc gataaaattg
     1261 cagattataa atacggtgaa aatggctttg tcagtgtcgt gaaacacgtc acctataacg
     1321 aaccgtatct actggggcat ttcccggaag atccgattat gcccggggtg attatttcgg
     1381 aaatcttcgg ccaggccagc gagtatctct ctttcctgac cgacatttgc gatatctggc
     1441 gcgaacgttt tgaggaagag ctgaagtcgt tacgtgatat tcacgcacgc attcaccgcc
     1501 ctgaaatgct ggagattatc cgtacccggc gcagccaggt acgcggcgtg ctggcggctc
     1561 agaacctgaa atttaaagat atcgcctatc cgggtgattc tattgatgtt gtcagcaaat
     1621 tggctttttc ggatgccagt ggctttaaac actattccgt gaccgcgtac gtcggtaaaa
     1681 agctgattag tcagggcacc attatcaatt tccgtgaaac caagtaatcc aataagagag
     1741 taaatattat gtctaccatg aatgacgcta ttgaacagcg taaagaagtt ctgatgacca
     1801 ttaaaactga gatcatccag cgactgaata ttcaacgcga tgaagcgcaa attgatgatg
     1861 atactgcgct gtttggtaat ggttttaaac tggactccgt cgatgcaacg gaaattgtcg
     1921 ttctgctgga taaagtattt gatatcaaga tcagcgaaag tgacgacccg tcctatatgc
     1981 gcagtatcaa tagtctggcc accttcgtta ttcaaaagaa aaacgattaa ctacttatta
     2041 aagaattaat tatcaggaca cgtttatgaa atctcttgct gaatttcact tgaaaaataa
     2101 tgcggtaatg ggggtatata acaaccgtac cctaccttct tcttatcacg atgctatgac
     2161 cgaatataaa gccgttcgtg aaaacgcgct gctggtggac tattctcacc tctctattgt
     2221 tagcgtcatg ggcgatgatg cctgggcgct gatcaaccag ctggtttccg cagatgtctc
     2281 catcattcgt gatgagcagg caatctactc actggtgttg aatgaagagg gcaccattcg
     2341 cggagacgtc tatgtgctgt gcagcattga cggttattac ctgctttcag aagatatttc
     2401 ggctgccgaa ttgatcgcca gcatgaatac cattctcgaa aaagcggaag aactggatat
     2461 tcaatcgatg cctgaaattc aggatatgcg ggaaaacaac tggggggcaa tcctgctcga
     2521 aggcccatac tcctgggaga tcatgtctga aattcatggc tttgacgtga ttggcctgcc
     2581 ttactacgaa tacatgaata ccgaggagga tctgctgctc ttccgctgtg gtaaacacgg
     2641 tgagtatgcg tacatgacca tcggtgagca ggcgaaactg gcggagcagt gggaaaaact
     2701 gttaaccgtt ggcgagaaat acctgatgca gaccggcggc ctggattatc aaaaaatcgt
     2761 acgcctcgaa aatccgtgct gggatgccag cctctgggaa ggtcaggcgg tgaatccggt
     2821 tcagttgcag atgcagtggg cggtgcagta cgacaaagat gattttattg gcaaggacgc
     2881 ggtgacagag ctatcccagg agtataccgg taataaactc atcggcatga tcgcacagga
     2941 agaatgcgaa ggcattgagg ctggcgatcg cgtgctggtg gaaggtcaag acgtgggcta
     3001 tgtcgtgaag gcactctttt ccccagcgtt gcagcgtttc attgccctga ccctgctgga
     3061 aaaggattac gcctggtctg acatcagcgg ctacgaaatt caaactgctc acggaattat
     3121 tccggcgcaa tcaaaatgta tgccgtttat ttataacctg agcatgttgg ttagcccaac
     3181 tgagcacagt tatatcgacg cgtcaaaaaa taaaagcgca gcgtaatgaa tatcgccgcg
     3241 tgactatagc ttacgcggct taaattaacc ccaattggcg ggattgccag gtattattca
     3301 tgaaaacgaa caggaaaaga gttgttatta ccgggctggg gattttgtcc tcactggcgg
     3361 aaaatattca ggatttccgg gaagcattat taaataaaaa aaatggcgtt aaggatagcg
     3421 aacgctttgc aaaatggttt gaaaatgcgc gtgcggcaga agtgttgcat gacattgaat
     3481 actccgatct gaacgaagat gttatcgatt cactggataa cgcagcgctc tgggcctaca
     3541 aagtgggtaa agatgcgctg agtcaggccg gcctgatgga tagccgtgac accctcaaag
     3601 aaacaggcat gatcgtcggt gtttcgtccg cgggaacaga ggcatttttg ccgctgttcg
     3661 aacagcgcat ggaagatttc tcgctgcgta aagcactctt ttccggcgca ttctcatcct
     3721 gttgttccag cgtttcaacg ctgctgggct tacagggtgg ggttgaactg gtggcgaccg
     3781 cgtgtaccgc cagcccgaac gccgtgggga tggcctttga ctacatccag aatggtaaaa
     3841 gcaaaaccat gctggcagtg ggcactgagc ccatctatct gccgaccttt gccggattct
     3901 acgcactgaa tgtcatgcat ccagacactt gtacgccgtt ctcgggtcag tccggtatgt
     3961 cgattggtga aggcgctggc gctatcgtgc tggaagagta tgagcacgcc gtagcacgcg
     4021 gggcgaccat ttatggtgag atcctctctt acgccacttc ctgcgatgct ttccatgaaa
     4081 ccgggccaga tccgcgtgcc agcggagcgg tgcaggtcat gaacaaggca atggcaaatg
     4141 cgggcgtaac cccggagcaa atcgattacg tcaacgcgca cggtacgggt accgaagcca
     4201 atgaccgtat tgaaacgctg gcaatgaaaa aggtcttcgc taaccacgat aaactgctgg
     4261 tgagctccac caaatcttac tttggccaca acattggcgc agcggggatt gtggaactga
     4321 ttgcctgtct ggcaacgctt ccggataacc gcgtactgcc gacgctgaat ttcagccatg
     4381 cacgcccgaa ttgcgatctg gactatgtgc cgaatgagtt tcgcgatcgc aaagtgaaca
     4441 tcttcatgaa gaataactat gccttcggcg gcaacaactg ctgcatgatc ctgagcatgg
     4501 agcccgcgtc cattccggtc agtacctatg attcgaagcg tgtggctatc tcgggcgtcg
     4561 gtgcagtatc cgcaataggc cacacgctca accaggttct ggaaaacatc tgggcgcagg
     4621 accaccacgt tgaactgggt agcgtaacct tcccggacga tacactggaa gaagccaaag
     4681 agctgctggc cgtgctggat gaatcaaacc agtttgccga gctgtttgat gaagagtaca
     4741 gcttcagcga agctacgctt ccggaaacgg aaagcaactt taaaacgttc caggtttccg
     4801 ggctggagcc gcgtaaacac ctgcgtcgtt tcgatcagcg taaagcgacc cgtggcggca
     4861 cgtttgccct gatcgcactc accgaagcgc tggaacaggc gaagcgtaaa atcaaacgcg
     4921 acggcgatga gttgggcatg atcatgggga tgtcccgcgg cccgcaggaa accacctaca
     4981 aatatctgca aagtctgaaa cccgatccac gcaaagtacg tacatcggaa tttcctggct
     5041 cgctgatgaa tgcgatccca acgttctgcg ggatctctga agggatcaaa gggtatacca
     5101 ctacgcttgc gacaggggaa aactcggcgc tgggcgcgct gacctacggt tatgaaatcg
     5161 tccggcagaa cctgcaaccg caggtattgg ttggcggcgc ggatgagtac ttcccgtcga
     5221 tgtcgctgta tatggatgcg gtgacacaaa agatccacat gacggctgag gcgagcgact
     5281 atcagattta cggtaaagat ccgcaaggtt atgttccggg cgaaggggct tgcatgctgc
     5341 tgctggaaga tccgcagttg gctgtggcgc gtggcgctga ggttctcgcg gaagtggtgg
     5401 gctacggcaa gtcatgcagc aatacctact tcgacgcctc attgttggaa gaaaaatccg
     5461 cgtcgatggc gctggccatt gaacgcgccc ttacgcatgc gggcatctct gctggcgata
     5521 ttgatttggt atgtggtacc agcaacggta gcgatgacca ttctcgtatc gaaattgacg
     5581 cgatttacaa caccttcgcc gcgcaaaacc cggcggtgcc ggtagtgaac tataacgctt
     5641 gctttggttt tgtcgcctct gccgctggtt tgcttaacct cgccgtgatt actgactgca
     5701 ttaagaagca ggcagtacct gcgattccaa atacggtgga attctttgat gagcgtgtga
     5761 attttgtacg ccagtcgatg aaacttgcgc tgaaccatgt cctgcttgtt ggcgcaacgg
     5821 aaggcggcaa ctactacgcg ttcgtcatta agggataaga ctgtggacaa tgccgctatt
     5881 atttcgggtt acagcgtgtg gatgccgtat gccgaagact gttcgcagtt aattgacaat
     5941 ctgaaacagg ggaaacgcgt tgcgcgtaca ccctggttta cctccaacga ggaagccatt
     6001 aaatgcgggt ttaagggtaa tccgtcagtg gcgacgttga aacaggtgga cgatagcgca
     6061 ttggacctgc tgtctcagtt gatcgacgaa gccctggaac aggcaatgct ggataagcat
     6121 tgcctggccg ggcgcaacgt tcgcgtttat ctgaccggta ttgggccgcg tatcgacggg
     6181 ctggattaca aatctttcta taattacaac gatgtagaag atatcaactt aacgcaatcc
     6241 atcacaaatc tgcatgcctc aaagatgtcg caggacacga tttcaagcca tcttgcgcgc
     6301 aaatatcgct tgcagtattt gccgcctaac atgaactgca ccagtaactc gtcactgact
     6361 gcggtgcatc tggcgacgca gggcattgag cagggcggga ttgatctcgc gattgtgctg
     6421 aactgttcga aaattaaaac ccaggacatc tggttcctgg aaacgcaatc gatgctggac
     6481 agcgaacaag tgcagccgtt tggtgaaaac agtaaaggtg tggcgttcgc agaaggtttt
     6541 agcgcgctcc tactggaaag cgctcatcac cgccgggcgc gtcaacaaag tgagggcgta
     6601 agggtgcaaa cgacttacac ccaaatcagc gcgggccgca gcaacgatgc ctcctggctt
     6661 agcactaacg tgcacaaggt catgcaggcc gcgatgaagc aggctgaaat tgcgctggat
     6721 gatcttgcag ccattcttcc acacggcaac ggttcggcag tgagcgataa cgcggaggcc
     6781 aaagccatcg ccatgttcgc aggggagcga cagatccccg ttctcgccta taaagggcag
     6841 atcggctata ccgcaaccgg atctggtgtt gtcgatctga tcattggcca ccattcgttg
     6901 acgcatcatc aactgatcgc acctgttggc aacgacgtca ttatcgacag catggcttcg
     6961 ctggtactta cggacggcag cgtgacaaac catagcaaac gccatttgct gaaggttggt
     7021 gtgggtgtcg atggttcagt tattggcgtt gtcatgacaa atatgcaagc ggggcgcgcg
     7081 aaatgacgga aaagagtatc tacctgtctt cctgggcgct tacggaaccg gacgacattt
     7141 tttattttta tcagccgacc tggctcaacg cctgggaagc acatatgtac gaaaggggat
     7201 tatacggcac ccgtgctaat ggctggggac tgaagcgctt tggcgtggag aacgatattt
     7261 atgcaccaga acggatccgt aatccacaat atatgcctta ccttgaggcg atggagcagg
     7321 tgaccgattc tgcattacaa caaatgccga ctaaagaacg catgaagtta atgaaatcgc
     7381 gcacggcatt tatttatgcc gattcctggg gcgaatccgg ttcgtttgaa aaaatttcca
     7441 gtgcgctgca tatcgccact attgatacgc ttcccaagaa tttacttaag aagttttcga
     7501 taacggaacc gacttgcaag attcgcggcg aaaaacaggc attcgttcag gcgctgcgta
     7561 tggcgcagga ttatctcgcc tgggatgtat tcgataacgt ggtgatttgt gctgcctatc
     7621 gggcaattcc ggtgctggta ttctctgaag aagatgtggg taaaccgcct aagcgtttat
     7681 ttccccgtcg tgctgatgag gtgaatttat ctgttgaacg agtgggttgt tttattctca
     7741 gccagcgtga gagcgccatc aggctgcaat gcggggaata tcattccggc actgcgccac
     7801 agcatgctac ggatattgat gtcatggcct tcagcgaacg gcaacaaatg ccgtcccggc
     7861 ctgctgctgg cgttatcgac ctggtggccc attacggtga aagcggttgt ctgacgcccg
     7921 cgttgagtat ggattattta acccgtcata ttcagcccgg tggaaaaatg cgtacggtca
     7981 ttgccgataa acagtttggt tatcactact ttgaccttga ataccttgaa ggataacgac
     8041 agtcgtatgc aatctactat taaatcgttg cccgctattt cgcttagcaa tatttataag
     8101 tgttatggcg ctggggcagg aaaggttgat gcgcttaaaa gcattaatct tgatatcaat
     8161 aaaggcgaat ttctcgcact gtgtgggccg tcaggcagtg gtaaaag
//
LOCUS       DBFIFM010000195         8206 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.195, whole genome shotgun sequence.
ACCESSION   DBFIFM010000195 DBFIFM010000000
VERSION     DBFIFM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.195"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            190..1569
                     /gene="ygjG"
                     /locus_tag="N7C74_003892"
     CDS             190..1569
                     /gene="ygjG"
                     /locus_tag="N7C74_003892"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HGU2433673.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(1611..1943)
                     /locus_tag="N7C74_003893"
     CDS             complement(1611..1943)
                     /locus_tag="N7C74_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HGU2433674.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            2162..3145
                     /gene="ebgR"
                     /locus_tag="N7C74_003894"
     CDS             2162..3145
                     /gene="ebgR"
                     /locus_tag="N7C74_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HGU2433675.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            3329..6421
                     /gene="ebgA"
                     /locus_tag="N7C74_003895"
     CDS             3329..6421
                     /gene="ebgA"
                     /locus_tag="N7C74_003895"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HGU2433676.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKT
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAIFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            6418..6867
                     /locus_tag="N7C74_003896"
     CDS             6418..6867
                     /locus_tag="N7C74_003896"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HGU2433677.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVDEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            6930..>8206
                     /locus_tag="N7C74_003897"
     CDS             6930..>8206
                     /locus_tag="N7C74_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU2433678.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRHTGIIVVSMLIAIFAVGFVAST"
BASE COUNT         1978 a         2175 c         2085 g         1968 t
ORIGIN      
        1 caattatcaa atccataccc gacaaaaacc gtgcaaaata acaacagatg ttaacaaata
       61 gcattaaata ttgcgcaaat gataaccaca tttgtgttta tcccgatttt cgcgatcgca
      121 gccggagtgg cgcaatccct gcaatactta aatcggtatc atgtgatacg cgagcctccg
      181 gagcatattt tgaacaggtt accttcgagc gcatcggctt tagcgtgcag cgcccacgcc
      241 ctgaatctca ttgaaaagcg aacgctggat catgaggaga tgaaagcact taaccgagag
      301 gtgattgaat acttcaaaga gcatgtcaat ccggggtttt tagagtatcg caaatctgtt
      361 accgccggcg gggattacgg agccgtagag tggcaagcgg gaggtttaaa tacgcttgtc
      421 gacacccagg gacaggagtt tatcgactgc ctgggaggtt ttggaatttt caacgtgggg
      481 caccgtaatc cagttgtggt ttccgccgta cagaatcaac ttgcgaaaca accactgcac
      541 agccaggagc tactcgatcc gttacgggct atgctggcga aaacccttgc tgcgctaacg
      601 ccaggtaaac tgaaatacag cttcttctgt aatagcggca ccgagtccgt tgaagcggcg
      661 ctgaagctgg cgaaggctta tcagtcgccg cgcggcaaat ttacttttat tgccaccagc
      721 ggcgcgttcc acggtaaatc acttggcgcg ctgtcggcca cggcgaaatc gaccttccgc
      781 aaaccgttta tgccactgct gccagggttc cgtcatgtgc cgtttggcaa tatcgaagcc
      841 atgcgcacgg ctcttaatga gtgcaaaaaa accggtgatg atgtggccgc ggtgatcctc
      901 gaaccgattc agggcgaagg tggcgtaatt ctgccgccgc cgggctatct caccgccgtg
      961 cgtaagctgt gcgatgagtt cggcgcactg atgatcctcg atgaagtaca aacgggcatg
     1021 gggcgcacgg gcaagatgtt cgcctgcgaa catgagaacg tacagccgga catcctgtgc
     1081 ctggcaaaag ccctcggcgg cggtgtgatg ccgattggcg caaccatcgc cactgaagag
     1141 gtgttctcag ttctgttcga caacccgttc ctgcatacca ccacctttgg cggcaacccg
     1201 ctggcctgtg cggcggcgct ggcgaccatc aatgtgttgc tggagcagaa cttaccggct
     1261 caggctgagc aaaaaggcga tatgttgctg gacggtttcc gtcaactggc gcgggaatat
     1321 cccgatctgg tacaggaagc gcgtggtaaa gggatgttaa tggcgattga gtttgttgat
     1381 aacgaaatcg gctataactt tgccagcgag atgttccgcc agcgcgtact ggtggccgga
     1441 acgctcaata acgccaaaac gatccgcatt gaaccgccac tgacactgac cattgaacag
     1501 tgtgaactcg tgatcaaagc ggcgcgtaag gcgctggcgg ccatgcgagt aagtgtcgaa
     1561 gaagcgtagt atcaaatcgg atggcaatac gacgtcgcca tccgatcttt ttatacgata
     1621 cgtacgcccg caggcatcat ccgctccggc gttaacagca cgctttcact gccgtcatcg
     1681 gtttccgcac acaacagcat gcattccgac gtttcaccac gcattttcgc tttttgcaga
     1741 ttgcataaga ccaccaccgt ttttcccatc aactcttctt cgctgtagta cggcaccagg
     1801 ctggtcacgg tttgcagtgt tttttccccc acatcaacct gtacgatgta cagcttgtcg
     1861 gcgttttcat ggcgtttcac ttccacaatc tttccgacgc gcatttccag acgtgcaaaa
     1921 tcagcgtaag ccacggtttc cattgcttcc tcccttcgag taaaatttta ctaaactata
     1981 gcaaaagttt ttctcaatcc tgtaggctaa aaatggagaa tgcaggcgtg atcacattcg
     2041 tgagccgctg tgttaccgtt acagcgtcaa agaaacgcgc tttatttact gaaaacaggt
     2101 gacccgataa gcacttcctc tacaatgggg gcgcacatca gggaaagtaa aaaaggtaaa
     2161 catggcaaca ctaaaagaca tcgcaatcga agctggcgta tccctggcga cagtatccag
     2221 ggtcttaaat gacgatccga cattgaatgt gaaagaagag acgaaacatc gcattctcga
     2281 gatcgccgaa aagctggagt acaagaccag tagcgcccgt aaactacaga caggcgcagt
     2341 caaccaacac catattctgg ctatctacag ctaccagcag gagctggaga tcaacgatcc
     2401 ttactatctg gcgatccgcc acggcattga aactcagtgc gaaaagctgg cgatcgaact
     2461 gaccaactgt tatgaacaca gcggattacc agacattaaa aacgtcaccg gtattttaat
     2521 tgtcggcaaa cccacgcccg ccctgcgcgc cgctgccagc gcgttgaccg acaatatctg
     2581 ttttatcgac tttcacgaac ccggcagcgg ttacgatgcg gtggatatcg atctggcacg
     2641 catcagtaaa gaaatcatcg acttctatat caaccagggc gttaatcgta ttggttttat
     2701 tggcggtgaa gatgagcctg gcaaggcgga tattcgtgag gtcgcctttg cggaatatgg
     2761 ccgactgaaa caagtggtac gcgaagagga tatctggcgc ggcggttttt ccagttcgtc
     2821 gggttatgaa ctggcaaaac aaatgctggc gcgggaagac tatccgaagg cactgtttgt
     2881 tgcttccgat tccattgcta tcggcgtact gcgggcgatt catgaacgtg gtctgaacat
     2941 cccacaggat atttcgctta tcagcgttaa cgatatcccc accgcgcgat ttacctttcc
     3001 gccgctctcc accgtgcgca tccattccga aatgatggga agtcagggcg taaacctggt
     3061 gtatgaaaaa gcccgcgatg gtcgcgcgct gccgctgtta gtcttcgttc ccagcaaatt
     3121 aaaactgcgc ggcacgaccc gttaaatccc cttacacact gtccggcaat cgtttttgcc
     3181 gggcagtgct gccgtttatt ttcgtgatcc agttaaagta aatgcattta cccgctactt
     3241 tttagtaaaa attttactaa actccccagc aattacacaa actaccatca ccatgaatgg
     3301 ttccgatttc tctctaccgg gaggccctat gaatcgctgg gaaaacattc agctcaccca
     3361 cgaaaaccga cttgcgccgc gtgcgtactt tttttcatat gattctgttg cgcaagcgcg
     3421 tacctttgcc cgcgaaacga gcagcctgtt tctgccctta agcggtcagt ggaatttcca
     3481 cttttttgac catccgctgc aagtgccaga agccttcacc tctgagttaa tggctgactg
     3541 ggggcatatt accgtccccg ccatgtggca aatggaaggt cacggcaaac tgcaatatac
     3601 cgacgaaggt tttccgttcc ccatcgatgt gccgtttgtt cccagcgata acccaaccgg
     3661 tgcctatcaa cgtattttca ccctcagtga cggctggcag ggtaaacaga cgctgattaa
     3721 atttgacggc gtcgaaacct attttgaagt ctacgttaac ggtcagtatg tgggtttcag
     3781 caagggcagt cgcctgaccg cagagtttga catcagcgcg atggttaaaa ccggcgacaa
     3841 cctgttgtgt gtgcgcgtga tgcagtgggc ggactctacc tacgtggaag accaggatat
     3901 gtggtggtca gcggggatct tccgcgatgt ttatctggtc ggaaaacacc taacgcatat
     3961 taacgatttc actgtgcgta ccgactttga cgaagcctat tgcgatgcca cgctttcctg
     4021 cgaagtggtg ctggaaaatc tcgccgcctc ccctgtcgtc acgacgctgg aatataccct
     4081 gtttgatggc gaacgcgtgg tgcacagcag cgccattgat catttggcaa ttgaaaaact
     4141 gaccagcgcc agctttgctt ttactgtcga acagccgcag caatggtcag cagaatcccc
     4201 ttatctttac catctggtca tgacgctgaa agacgccaac ggcaacgttc tggaagtggt
     4261 gccacaacgc gttggcttcc gtgatatcaa agtgcgcgac ggtctgttct ggatcaataa
     4321 ccgttatgtg atgctgcacg gcgtcaaccg tcacgacaac gatcatcgca aaggccgcgc
     4381 cgttggaatg gatcgcgtcg agaaagatct ccagttgatg aagcagcaca acatcaactc
     4441 cgtgcgtacc gctcactacc cgaacgatcc gcgtttttac gaactgtgtg atatctacgg
     4501 cctgtttgtg atggcggaaa ccgacgtcga atcgcacggc tttgctaatg tcggcgatat
     4561 cagccgtatt accgacgatc cgcagtggga aaaagtctac gtcgagcgca ttgttcgcca
     4621 tattcacgcg cagaaaaacc atccgtcgat catcatctgg tcgctgggca atgaatccgg
     4681 ctatggctgt aacatccgcg cgatgtacca cgcagcgaag gcgctggatg acacgcgact
     4741 ggtgcattac gaagaagatc gcgatgctga agtggtcgat attatttcca ccatgtacac
     4801 ccgcgtgccg ctgatgaatg agtttggtga atacccgcat ccgaagccgc gcatcatctg
     4861 tgaatatgct catgcgatgg ggaacggacc gggcgggctg acggagtacc agaacgtctt
     4921 ctataagcac gattgtattc agggacatta tgtttgggag tggtgcgacc acgggatcca
     4981 ggcgcaggat gacaacggca atgtctggta taaattcggc ggcgactacg gcgactatcc
     5041 caacaactat aacttctgtc ttgatggttt gatctattcc gatcagacgc cgggaccagg
     5101 cctgaaagag tacaaacagg ttatcgcgcc ggtaaaaatc cacgcgctgg atctgactcg
     5161 cggcgagctg aaagtcgaaa ataaactgtg gtttaccacg ctggatgact acaccctgca
     5221 cgcagaggtg cgcgccgaag gtgaaacgct cgcgacgcaa cagattaaac tgcgcgacgt
     5281 tgcgccgaac agcgaagccc ccttgcagat cacgctgccg cagctggacg cccgcgaagc
     5341 gttcctcaac attacggtga ccaaagattc ccgcacccgc tacagcgaag ccgggcattc
     5401 tatcgccact tatcagttcc cgctgaagga aaacaccgcg cagcctgtac ctttcgcacc
     5461 gaataacgcc cgtccgctga cgctggaaga cgatcgtttg agctgtactg ttcgcggcta
     5521 caactttgcg atcaccttct caaaaacgag tggtaaaccg acatcctggc aggtaaatgg
     5581 cgaatcgctg ctgacccgcg agccaaagat caacttcttc aagccaatga tcgacaacca
     5641 caagcaggag tacgaagggt tgtggcagcc gaatcatttg cagatcatgc aggaacatct
     5701 gcgcgacttt gccgtagaac agagcgatgg tgaagtgttg atcatcagcc gcacggttat
     5761 agcaccgccg gtgtttgact tcgggatgcg ctgtacctac atctggcgca tcgctgcaga
     5821 tggccaggtt aacgtggcgc tttccggcga gcgttacggc gactatccgc acatcattcc
     5881 gtgcatcggt ttcaccatgg ggattaacgg cgaatacgat caggtggcgt attacggtcg
     5941 tggaccgggc gaaaactacg ccgacagcca gcaggctaac atcatcgata tctggcgcag
     6001 caccgtcgat gccatattcg agaactatcc cttcccgcag aacaacggca accgtcagca
     6061 tgtccgctgg acggcactga ctaaccgcca cggcaacggt ctgctggtgg ttccgcagcg
     6121 cccaattaac ttcagcgcct ggcactatac ccaggaaaac atccacgctg cccagcactg
     6181 taacgagctg cagcgcagtg atgacatcac cctgaatctc gaccaccagc tgcttggcct
     6241 cggctccaat tcctggggca gcgaggtgct ggactcctgg cgcgtctggt tccgtgactt
     6301 cagctacggc tttacgttgc tgccggtttc tggcggagaa gctaccgcgc aaagcctggc
     6361 gtcgtatgag ttcggcgcag ggttcttttc cacaaatttg cacagcgaga ataagcaatg
     6421 aggatcatcg ataacttaga acagttccgc cagatttacg cctctggtaa gaagtggcaa
     6481 cgctgcgttg aagcgattga aaatatcgac aacattcagc ctggcgtcgc ccactccatc
     6541 ggtgactcat tgacttaccg cgtggagaca gactccgcga ccgatgcgct atttaccggg
     6601 catcgacgct attttgaagt gcattactac ctgcaagggc agcaaaaaat tgaatatgcg
     6661 ccgaaagaga cattacaggt cgtggaatat tatcgtgatg aaactgaccg tgaatattta
     6721 aaaggctgcg gagaaaccgt tgaggtcgac gaagggcaaa tcgttatttg cgatatccat
     6781 gaagcatatc ggtttatctg caataacgcg gtcaaaaaag tagttctcaa agtcaccatc
     6841 gaagatggtt atttccataa caaataacaa ctacggcggc aaaaggagtt tgccgccacc
     6901 gctaccctac tcattttcgg agatgtgtta tgtctgatac caaacgtaat acaatcggca
     6961 aattcggctt gctctcgctg acttttgccg ccgtttacag ctttaacaac gttatcaata
     7021 ataatattga gcttggactg gcctcggcac cgatgttttt cctcgcgacg attttttatt
     7081 ttattccctt ctgtctgatc atcgcagaat ttgtttcgtt aaataaaaac tcagaagccg
     7141 gtgtctacgc gtgggtaaaa agttcgctgg gcggacgttg ggcatttatt actgcctata
     7201 cctactggtt cgtaaacctg ttctttttca cctcgctgtt gccgcgcgtt attgcttatg
     7261 cttcgtatgc cttcctcggc tatgaatata ttatgacgcc ggttgccacc accattatca
     7321 gtatggtgct gttcgccttc tccacctggg tttccaccaa cggggcgaaa atgttggggc
     7381 caattacctc cgtcacttca acgctgatgc tgctgttaac gctctcctac attttactgg
     7441 caggtacggc gctggttggc ggcgtacagc ctgctgaccc catcaccgtt gacgcgatga
     7501 tcccgaactt caactgggcg ttcctcggcg ttaccacctg gatctttatg gccgcaggtg
     7561 gcgcggagtc cgtcgctgtg tacgttaacg acgtcaaagg cggttcgaaa tcgttcgtta
     7621 aagtgatcat cctcgccggg atttttatcg gtgtactata ttccgtctcc tcggtgctga
     7681 ttaacgtctt cgtcagcagc aaagagttga aatttactgg cggatcggtg caggtattcc
     7741 acggcatggc ggcgtatttt ggtctgccgg aagcgttgat gaatcgcttt gtcggtctgg
     7801 tgtcctttac cgcaatgttc ggttccctgc tgatgtggac cgcaacgccg gtgaaaattt
     7861 tcttctccga aatcccggaa ggcatctttg gtaagaaaac cgtcgaactt aacgaaaacg
     7921 gcgttccggc gcgcgcagcg tggatccagt tcctgatcgt catcccgctg atgattatcc
     7981 cgatgctcgg ttccaatact gtgcaggatc tgatgaatac tattattaat atgaccgccg
     8041 cagcgtccat gcttccgccg ttattcatca tgctggctta cctgaattta cgcgccaaat
     8101 tagatcacct gccacgcgat ttccgtatgg gctcccgcca caccggtatt atcgttgttt
     8161 caatgctgat tgcgatattt gccgtagggt ttgtcgcttc gacatt
//
LOCUS       DBFIFM010000196         8176 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.196, whole genome shotgun sequence.
ACCESSION   DBFIFM010000196 DBFIFM010000000
VERSION     DBFIFM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.196"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(58..573)
                     /gene="ogt"
                     /locus_tag="N7C74_003898"
     CDS             complement(58..573)
                     /gene="ogt"
                     /locus_tag="N7C74_003898"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HGU2433679.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGELAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(584..2110)
                     /gene="abgT"
                     /locus_tag="N7C74_003899"
     CDS             complement(584..2110)
                     /gene="abgT"
                     /locus_tag="N7C74_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HGU2433680.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVYPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            complement(2147..3592)
                     /gene="abgB"
                     /locus_tag="N7C74_003900"
     CDS             complement(2147..3592)
                     /gene="abgB"
                     /locus_tag="N7C74_003900"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HGU2433681.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAIKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQ
                     ATLTSNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            complement(3592..4902)
                     /gene="abgA"
                     /locus_tag="N7C74_003901"
     CDS             complement(3592..4902)
                     /gene="abgA"
                     /locus_tag="N7C74_003901"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HGU2433682.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNENSRMGLPDELTLQREFERARQQGALAQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTTIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGYNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            5078..5986
                     /gene="abgR"
                     /locus_tag="N7C74_003902"
     CDS             5078..5986
                     /gene="abgR"
                     /locus_tag="N7C74_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2433683.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            6316..6879
                     /gene="smrA"
                     /locus_tag="N7C74_003903"
     CDS             6316..6879
                     /gene="smrA"
                     /locus_tag="N7C74_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HGU2433684.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6900..8132)
                     /gene="dgcM"
                     /locus_tag="N7C74_003904"
     CDS             complement(6900..8132)
                     /gene="dgcM"
                     /locus_tag="N7C74_003904"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HGU2433685.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
BASE COUNT         2080 a         2067 c         2052 g         1977 t
ORIGIN      
        1 ttgttaaggg taactttaca taaaagtgtg aacaagctgg cacaaattgt ttaatgttta
       61 cagcaaaaga taaccttcgt ggcgcaataa ccactctttt cgctgaactc cgcctgcata
      121 tccggtcatg gtgccgtttc ggccaataac ccgatggcaa ggtacgacga tgctgatggg
      181 attcgatccg tttgccgcac caacggcacg cgccgcgcca ggacggccca attgctcagc
      241 cagttcgccg taatgcatta cctgcccgca ggggatagtg cgtagtgttt tccagacttc
      301 gcgctgaaat ggcgtccccc ccgtagcagt gggaagcgta tcaataatgc taagattacc
      361 ggcaaaatat tcacgaagct tgtcgcttaa accgcctgga ttggtggcag aaatgcgctc
      421 atagccttct ttgcgataat ggatgtccag cagctgcacc atgcgttcgc tgtactcttc
      481 ccattcaacc gcccgcaggc gaaattgctc atcgcaaatc acccacagtg gacccagtgg
      541 tgtggcaatt ttttcttcaa gtaatctcag catccgttct ctcttaagac aaacgtgggt
      601 aaatacccgg acctatcggc aggcccacaa gataccacgc cagcaacatc agcagccata
      661 ccaccaaaaa gataagcgga tagggcaaga ctaacgaata gtaagtaccc agtttcgcgt
      721 ctggtttgta gcgttgcagg aatccaagaa acagtggaac aaaaggatat actggcgcta
      781 aaggcaatac ggatgagtcg gcaatacgaa agaggatttg cgcaaatgcc gggtgaaagc
      841 caagtagcat aaacattggt acgaaaatgg gggccagaat cgaccagatt gcggaaccgc
      901 tggcgataaa catgcataag aaagaggaaa gcaacgccag accgacaaac gccgggatgc
      961 cgctaagccc tgaactttcc aggatatcgg tcagccccac ggcgatgaat ttccccatgt
     1021 tgctccagtt aaacatggcg acaaactggg cgaggggaaa aaccatcacg ataaatcccg
     1081 ccatctcttt catcggttca atcattaaat gcggtaaatc cgcctgacgt cgaattgtgc
     1141 gggtagcgat gccataagcc agcgagacaa caaagaaaaa aagaatgatc agtggcacga
     1201 tacctttaat aaagggtgat ggcatcacgg tgtgattaat cggatcgcgc aatatcccgt
     1261 tttccgggat caccatcagc gcaatcgcag caataaaaag tagcgatacg acacctgcta
     1321 tgcgtaaacc aaaacgctga ctttcggtca atgtctgcag tttctcatcg ctgtttccct
     1381 gccattgacc taaccgtggc tcgatgattt tgtcggttat caggccgcca acaatcgtca
     1441 gtacgactac ggagctggcc ataaaatacc agttatcaat tacactgacg tgcatttgcg
     1501 gattgaacgc agctgccgct tccgtgctga tccccgacag caacacgtcg gttgtgacaa
     1561 tcagtaaatt agccgtaaag ccgcaaccta cgcctgcaat ggcagccagt aaacctgcaa
     1621 ctggatgcct gcccaccgcc agaaaaatca gcgcacccat cggtggcatg atcactaacg
     1681 ccgcatcgga agaaatgtgg ctgaaaaaag caataaacag caccatataa ctggcgtagc
     1741 gggcattaac atgcgatgcc attttaacca ttagtgctgg cagtaagccg acgcgctccg
     1801 ccagaccggc acctaaaacc agcgccagga tcgcaccaag tggagcaaaa ccgctaaagt
     1861 ttttaataac attgggtaaa aaccagtgta atccttccac actgagcagg tttttcacca
     1921 ccaccggcgt accatcggtc gggtttttcg cactgacgcc aaaggccgac aaaattgccg
     1981 tcgtcaccat gagtacgata atcaaataga taaagagcaa aaaaggatgg ggaaccttgt
     2041 taccaattct ttcgacccag ccatagagct tcccggattg ggaggacgac ggtatggatg
     2101 acatactcat gggcattcct cggttgttgt gtttgcgttg ttgttgttat tttaaaggtg
     2161 acggtgtcac gtttttcggg atagggcagt gatacggttg cgtgtccgta acttgctgat
     2221 gctcttgttg gcattcttgc aatagccctg aatcaatgaa gagattgagt gtggttgctg
     2281 ccatagtttt cgccgccagc agcattcctt tatgagcaat agatgttcgc ccctggctaa
     2341 ccagttgcca cgtatgtagg ggtgtaccga cggcaaaaca ggggctgaaa cactgggcaa
     2401 caggcagttt ccaactgacg tcgccgacat cagtcgatgc cgcaagcacg ttatcggtgg
     2461 cggcatatgg agcgacttca ttcgccagta ccgtttcacg atgacgtagt gcaaaaacct
     2521 tgccgttttc gccaccggtt gcggcgatat tattcagact gttttgccga tcgttggagg
     2581 taagcgtagc ctgaatttgt ttcgcaaaag ccagttcttc ggagttccat tccggggtac
     2641 caaaatggga tagggcctgg tacatggcat tttctaaggt gcgattcggg agataactgg
     2701 aacaggcttt gtcgaagcgg cattcaaccg tggtttcggt catcaatgcc gcaccttcgg
     2761 cgattttggc gacccgatca taaatatgct gcacgtcggt catttcgggg gcgcggataa
     2821 gataaagcac ttctgcctgc gcctggacca cgttgggcga gatcccgcca ctatttgtga
     2881 tggcatagtg tacgcgcgct ttttcaataa tatgttcgtt gaggaagttg gtgccagtgg
     2941 tcatcaacgt tacggcatca agggcgctgc gtcccaaatg aggggaattc gcggcatgtg
     3001 ctgcgatccc tttaaagcgc catgatgcct gaatgtttgc cagcgtacgg gtattgaaca
     3061 taccggcaaa ggcttccggg tgccaggtga gtgccgcatc cacatcatca aataccccct
     3121 cgcggaccat gaacgtttta cccgagccgc cttcttcgcc aggacaacca taaaagcgca
     3181 ctgtgccgcc ttgcccatat tgttccagcc atttcttgat ggctattgca gcggcaaagg
     3241 cggcggttcc cagcaaattg tgtccgcaac cgtgaccatt ttcaccgggc gtcacggatg
     3301 taggttgcgc gcaacctgct tgctgactta aacctgccag ggcgtcatat tctcccagca
     3361 gggcgataac cggtttgcct tgaccaaacg aagcaataaa ggcatttggg atattgccta
     3421 cgttgcgggt aacggtgaag cctgcagatt ccagcgccga agccagatgc tccgctgacc
     3481 agaactcttc aaaacgtgtt tctggatgat cccagatttg atcggcaata tcggtataac
     3541 gttggcgatc ggcttcaatc gcatcgtcga taaaacgata gatttcctgc atcagatacc
     3601 tcgcgtccag ggaaaattga gcgcggtgcg cgccagcgtt tcgacggcaa tagcgagaac
     3661 ctgctcgtca aaatcgaatt tttcgttgtg atgacctgcc gccagctgtg tgccaaacac
     3721 cacgtaggag gcttgccctt gatgttgctg cacgcgggcc atcattaatg tggcatcttc
     3781 ggaacccgca ggcgcttcaa cacgttcaat ggcctgattg acccccgcga cctgagccgc
     3841 ctgactttgc aaccatgcga cccattgcgg cgaaggagaa ctggcggtag ctgcacccat
     3901 cagacgagtt tcaacgccga caccatacat ggttgctgcg ccctgaatcg cttgctgagc
     3961 acggtcaaaa acatattgat taatgacgtc gctggccccg cgtgtttcca ctttcagcaa
     4021 cgccgaggca ggaacaacgt tacgaccgct tcctgcctgc ataacgccca cgtttactct
     4081 ggaagctcct tcgctgtgcg gggcgattgc atgcagtgca agagtggctt gtgctgccgc
     4141 caacaaggca ttgtaaccgt cttctggttt tgcgcctgcg tgagcggcgg taccggtgaa
     4201 gtgcgcgtca aatttggtgg ttgccataaa attatcactg ccgcacacca cggtgcccgc
     4261 aggtacgcca gtgccaatgt gcacggcagt aaaataatca acatcatcta cgacacctgc
     4321 atcgaccatc gcccgcgcgc cacgcgtacc ttcctctgca ggctgaaaaa tcagtttgat
     4381 gacgccatgt agtccggact cgaattgttt aagggtatgc gccagcccaa gcccaatggt
     4441 ggtatgccca tcatgaccac aggcatgcat cattccggcg ttacatgacg caaaaccgtc
     4501 gcggtagggg cgatggctga catcctgctc ttcactgaga tccagcgcgt ccatatcgac
     4561 acggaaagcc atcaccggac cggggcgacc ggtatccagg gtggcgacga tacccgtgaa
     4621 accaccttca aaagccgcaa tccattgtgc tagcgcaccc tgttgacgag cgcgctcgaa
     4681 ttcgcgttgt agagtgagtt catcaggtag tcccatccgg ctattttcat taactacttc
     4741 gcggcccagc gccagtgagt agccgagctg ctgcaattct tccgcaacaa gggtggcagt
     4801 gcggaattcc acccagccag actctgcata gtgatgaaaa tcacgtcgcc agtgcgataa
     4861 ttttggggca agcgaattaa caaattgatt caaagactcc ataacctttc ccgtcatcag
     4921 taaaaagtgt gacccggttc acgtagcgat agtttttact tatcactaac tgatttttca
     4981 cagttttaac cgttcataaa ttaccctgac acaatcatct gcattaaagt agatgccagt
     5041 ttctttggtc tgataaataa cggttatcgg tggcgtcatg gcttttcagg taaaaattca
     5101 tcaaattcgg gcttttgttg aagtggctcg tcagggcagc attcgcggag cgagccgaat
     5161 gttgaatatg tcgcaaccgg cactgagtaa atctattcag gagctggaag aagggttagc
     5221 ggcgcaactc ttttttcgcc gtagtaaagg cgtgacgtta actgatgccg gtgaaagttt
     5281 ttatcagcac gccagtctta ttcttgaaga actgcgcgca gcccaagagg atattcgcca
     5341 acgacaaggg caactggcag ggcagattaa tatcggcatg ggggccagta tttcccgcag
     5401 tctgatgcca gctgtaatat ctcgttttca tcagcagcat ccgcaggtaa aagtacgcat
     5461 tatggaaggg caactggtgt cgatgattaa tgaattgcgt cagggagaat tggatttcac
     5521 catcaatacc tattatcagg gaccgtacga ccacgaattt acttttgaga aattactgga
     5581 aaagcaattc gcgatctttt gccgcccggg acaccccgcc attggtgccc gttcgatcaa
     5641 acagttactg gattacagct ggacaatgcc gacgccacac ggcagctact acaaacagtt
     5701 gagtgaattg cttgacgatc aggcgcaaac gccacaggtc ggtgtagtct gcgagacgtt
     5761 ctcagcctgt atcagtctgg tggcaaaaag cgattttctc agcatactgc ctgaagaaat
     5821 gggctgcgat cccttgcacg gacaggggct ggtgatgttg ccggttagcg aaattttacc
     5881 gaaagcggcc tattatttga ttcagcggcg tgatagtcgc cagacaccac tgaccgcgtc
     5941 attaatcacg caattccggc gagaatgcgg ctatctgcaa agttaaaact gcataaaaaa
     6001 atagagtctg tcgacatccg ccagactcta cagtacacac agcagtgcat ccgcgtctta
     6061 aatccggcat tgtctcctct gcgccggtga ctgtgttata acggttttaa cctttagttg
     6121 ccaattttct tcagtgacag atttcacgaa aatataagtt acattattaa tatcgtgaat
     6181 gaataatcat gcataagcat tttgcttaaa atatcggcaa tatttggaac ttattactgg
     6241 aattttgggt aatacgttgt tggaccgacc cggtctggtt atcatatcgc gctcttaatt
     6301 gcgggaggat gtaacatgaa ccttgacgac aaatcgctgt ttcttgacgc catggaagat
     6361 gtccagccgc tgaaacgtgc taccgatgtc cactggcatc caacgcgtaa ccaacgtgcg
     6421 ccgcagcgta tcgacacgct gcaacttgat aatttcctca ccaccggatt tctcgacatc
     6481 atcccgctaa gtcagccgct ggagtttcgg cgggaagggt tgcaacatgg ggtgctggat
     6541 aagctgcgca gtggtaaata tccgcaacag gcgagcctga atcttttgcg ccagccggtg
     6601 gaagagtgcc gcaaaatgat gttcagtttc attcaacaag ccatggcgga tggtttgcgt
     6661 aacgtgctga ttattcatgg taaagggcgg gatgataaat cgcatgccaa tattgtccgc
     6721 agctatgtgg cgcgctggtt gaccgaattt gatgatgtcc aggcatattg taccgcgcta
     6781 ccgcatcatg gcggcagcgg ggcgtgttac gtcgcactac gtaaaacggc acaggcaaag
     6841 caagaaaact gggagcgcca cgctaagcgc agtcgttgat ctcgagacgc atccgtggct
     6901 tatgccgcca gcacgcggtt gcgtccatca tttttcgccc gatacaaagc atcatcaacg
     6961 cgtttaaaca gttcatcgat gctttcattt ccttcgtgat gcgccacacc aatgctgacg
     7021 gtaaagcgtg gtaagcccga aatactcact tttgccacgc ttacgcggat agtttcagcc
     7081 agcgaaagcg cggtatccag tggggttctt ggtagtaata agacaaactc ttcgcctccc
     7141 caacgaaaca ccaaatcgcc tttgcgagcg caactttcga gagtgcgggc gagggcgcat
     7201 aacacctcat cacctttaga atgcccatag agatcgttaa tgtgtttaaa acgatctgtg
     7261 tcgatgagca ataagctgta atcctgagcg atggcgaggt gctgcatttg gcctggttcc
     7321 gtaatgtgat aaaactgtcg ccgattcagt aatccggtca tcgcgtcatg gtgagcagca
     7381 tgttccagct gctcctccag ccgtttttgc tcagtaatat catgcacaat acataacatg
     7441 agcttgtcgc cataaatttc aatcggtccg gcataggtct gcacatgacg agtcgaacca
     7501 tccgccagct tatgaataaa attcagaggt ttatgaccac cgggtaaatg cgagatttca
     7561 tgcatgatag gcatgacgcg acgcccgagc atatttattt cccaggtatg tttctggcac
     7621 atcgtttcat ggttataacc atagaaattg agcgcggcga ggttagcatc gacgatttgt
     7681 ccatctcgtg acgggtcaat caacaacatt ggtgcagagt tagtcagaaa aaagcgcgca
     7741 taaaaacctt gttttttgcg ctgataattt gccgagcgac tggcttttaa acccagcgtt
     7801 gccggcgctt cgataccttc gaaaataatc accggttctg tttctgtcag ctctcgcaaa
     7861 acaagccgac agcttaatgc tgtttcctct tctttacgct gaacagtgag gatttcgata
     7921 atatcgtgtt ggttttgcag atcggagagg tatttcggca gttctttttg tgaggagacg
     7981 gaatagggac cggttcgtag ctgactaaac gtgaggtctt gcatcaacag tcgcgccgcg
     8041 ctgttggcat aaattaactg ttcctcaaag ggcgaaacga tccagacagg actggtgagt
     8101 aagtccagag tattgaagtt gtgcgtaatc attgagatcc cattattttt atcaattttt
     8161 gttgctatcc gatcgc
//
LOCUS       DBFIFM010000197         8118 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.197, whole genome shotgun sequence.
ACCESSION   DBFIFM010000197 DBFIFM010000000
VERSION     DBFIFM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.197"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..454
                     /locus_tag="N7C74_003905"
     CDS             <1..454
                     /locus_tag="N7C74_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU2433686.1"
                     /translation="LAVIRGKHLGFIFQFFNLMPVLNVFDNVYFPLVLNGQFSKKEAT
                     ERTLHYLDSVGLSGFGDRKPGQLSGGQQQRVAIARALAHEPMVVIADEPTGNLDLATG
                     EAILDLLLTINQQTGTTFVISTHSSELKARARRVVEIQDGVLIHDSDA"
     gene            438..1094
                     /locus_tag="N7C74_003906"
     CDS             438..1094
                     /locus_tag="N7C74_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433687.1"
                     /translation="MTQMHKGFLLIGVFLYAAFVAPKIYAATPDAVNQVLQQGLPAQP
                     AALATLTEALKKEYEARHNVVALVFYAYGLLRQADGYAATNDFIHASEYAKSGFFWLD
                     EAVDLQEKNQRVRYLRARVDAYLPADSGRCVVTVKDTEHMLADPAIWTTTIRDHILAM
                     RYRALRHCKDTTRANALLAQIKGQNAALAQSLTQDFNVVPEWDSEELTQVLLPLMKGE
                     "
     gene            1095..2405
                     /locus_tag="N7C74_003907"
     CDS             1095..2405
                     /locus_tag="N7C74_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2433688.1"
                     /translation="MSGEILAVNCVLLLILFFVFYQLLFYSRDVKFAFLNLFRHKRRS
                     FSTITAIVLGGVAIFLYGGFIDYSFWILKEQTIRTNIGHVQIYNQHYFETSNKNKSLI
                     ADYAALKKAILSDPTLAADISTLSGQLEFTGVISHYESETSSYFSALGGEPLPALKLG
                     SFDKIIAGSDLSRVKGDEITLGSGLAKTLNAKYDDWLDVMVVNTAGGQGALSLKMRGI
                     FESGIKDYDDVAMKIPLDTAQRMMGTDGVSKVLILLKEDDTAAFSAKLRQFIERNHLP
                     LVVKDWRDVSLFYQQVEGLLSGIYFFIKLIVALIVIFMIGNSMAMNIVERTREITTLR
                     AIGLKPLHVTRLFLTEGIFIGVIGAVGSLLVGGVLAWIINLYGIAMPPSPGQTIGYTA
                     FIKTSNPELIWVTVVLPILTATGASVLPALRASRLNISDAFKFS"
     gene            2414..3202
                     /locus_tag="N7C74_003908"
     CDS             2414..3202
                     /locus_tag="N7C74_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HGU2433689.1"
                     /translation="MNMAFYGKWFACLWLATSCVQAASTDNKALEIIRRADEIRSPNK
                     PFRYTLTVTEYKAGATQPENKQVLDISMRFMKPQGNEKADARSLVRFIYPPRDKGKIM
                     LSDWYDLWFYTPELRRPMPISRQQRLIGQISNGDVIVTNFEYAYDSTLMGEVTCAEKQ
                     CYKLALVRKSADITWPKVIYYVEKDGDNRPWKAAYYSQDDQLIKEVLYQDFQPVLGKT
                     RPMKITVTDVRHGNNYSVMEYSDVRLESLPEFHFTKEYIQRGAK"
     gene            3199..4587
                     /locus_tag="N7C74_003909"
     CDS             3199..4587
                     /locus_tag="N7C74_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433690.1"
                     /translation="MKRTLKISSLLCVALPLTVQADCLSGDEVAQNSDVTLYEQVSYV
                     NKQASAWQIAGKNPYTRHNGYQEAGIGINSGCSIIDNTLDLKLNLYGINEYALKPAGK
                     FETDDSRTRALINRLSLVYSASDSVQFEAGKFAAPSGMFFLRSPSDLQTHYYTGFQST
                     RLHDPKMTSAYQASSWGAKMSVDTRDYAFSASVIPKLATIDKRYVTSGNWSANQQGNS
                     DEAYLLSFSDHRFGEHTPTVNVRLGPSPSLALSDSYHYTPQLTLNVDAAYHRSQQWRH
                     LSHRETAQVEEYQFPDSLYETKDESGVELALGGQYTSDNFSVFGVEYYFQSEGYSRAE
                     QRQQRELIDFLNTTTGYAPLDQAFDSYKYLMASEISNTANQGMLQGKHYLNAWASLPL
                     AGESTLQPSLVVNLVDGSTLLGLHYSTPLSAISNQLEAYAGGYSALGSRYSEFALFGD
                     TLGLYLGFKYYL"
     gene            4601..5542
                     /locus_tag="N7C74_003910"
     CDS             4601..5542
                     /locus_tag="N7C74_003910"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HGU2433691.1"
                     /translation="MNNASQPTVLLFAGQGNPVIGMGSDLWDLNASTKHIWDCASDIS
                     GLNLRRLCQKGPMNRLIQTTVQQLAVTAINVTLYTLCRERLENLTVVGSCGHSVGEYS
                     ALYAAGAISLETLFGMIHFRASLMHDESQRNKGAMLAVKGINREQLQTMITESGIALD
                     ISCDNTPRQQVIGGTQAALNEFATLLMAAGYEPVKLGVSGAWHTRLMEDGVQAMRDYL
                     AGLDIASPEHQVLMNVTAKSEVAPSIIKENLSLHLTHTVKWTESLDTFLNMPTSVAFL
                     EISNKPYLGNMFNDFAGVDQQRVMHCRKAFSDAKVFK"
     gene            5539..6525
                     /gene="ycdU"
                     /locus_tag="N7C74_003911"
     CDS             5539..6525
                     /gene="ycdU"
                     /locus_tag="N7C74_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HGU2433692.1"
                     /translation="MIPDYLTFIRFQDKRSLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQRLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVIEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSRRYAFLGRLFLQEIALFFSSENALSTFFAKPLWLRLFILLVIEVMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFYCRFLWHNDFMMACDMYFRWGHFNK"
     gene            6892..8094
                     /locus_tag="N7C74_003912"
     CDS             6892..8094
                     /locus_tag="N7C74_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2433693.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVAIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARI
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQIVNK"
BASE COUNT         2006 a         1772 c         2139 g         2201 t
ORIGIN      
        1 gttggctgtg attcgtggaa aacatttggg ttttattttt cagtttttta accttatgcc
       61 ggtattaaat gttttcgata acgtttattt cccactggtg cttaatggtc agttcagtaa
      121 aaaagaagcc actgaacgga cgctgcatta tctcgatagt gttggattaa gcggttttgg
      181 cgatcgtaaa cctgggcaac tatcgggcgg tcaacaacag cgtgtcgcca tcgccagggc
      241 actggcccac gaaccgatgg tggtcattgc cgatgaaccg acgggaaatt tagacctggc
      301 aaccggcgag gctattttgg acctgttgtt gacgattaat cagcagaccg gcaccacatt
      361 tgttatttcc acgcactcca gcgaattaaa agcgcgggca cggcgagtgg ttgaaattca
      421 ggatggagta ttgattcatg actcagatgc ataaaggatt tttgctcatc ggcgtctttt
      481 tgtacgcggc gtttgtggct ccgaagattt acgcggcaac acccgacgct gttaaccagg
      541 ttctgcaaca ggggctcccg gcgcagcctg ccgcgctggc gacgctgacc gaggcgttaa
      601 agaaagagta tgaagccagg cacaatgtgg tggcgctggt tttttacgcc tatggcttac
      661 tgcgtcaagc ggacggttat gcggcaacaa acgattttat ccatgcctca gaatacgcca
      721 aaagcggctt tttctggctg gatgaagcgg tggatttgca ggagaaaaat caacgcgtac
      781 ggtatttacg cgcccgcgta gatgcctatt tacccgcaga ctcagggcgt tgcgtggtga
      841 cagtcaaaga caccgaacac atgctggctg acccggcgat atggacgaca acgatccgcg
      901 accatattct ggcgatgcgt tatcgcgcat tacgtcactg caaggacacc acccgcgcga
      961 atgcgttgtt ggcgcaaatt aaagggcaaa acgccgcgct tgcgcagtcg ctgacgcagg
     1021 attttaatgt tgtaccagaa tgggatagtg aagagttaac ccaggtactg ctgccgctga
     1081 tgaaaggtga gtaaatgtcc ggggaaattt tagctgttaa ttgcgttctg ctgctgattc
     1141 tgttttttgt gttttatcag cttctgtttt attcacgaga tgtcaaattt gcatttttga
     1201 atctctttcg ccacaagcgt cgctcttttt ctaccataac cgctattgta ttgggtggcg
     1261 tggcaatttt tttatatggc ggatttattg attactcttt ctggatcctg aaagagcaga
     1321 cgattcgtac caatattggt catgtacaaa tttacaatca gcactatttt gaaacgtcga
     1381 ataaaaataa gagcctgatt gccgattatg cggcgttaaa gaaagctatt ttgtcggacc
     1441 cgacgctggc ggcggatatt tccacgctct ccgggcaact ggagtttact ggcgtaattt
     1501 cacattacga gagcgaaacc tccagctatt tctccgcgct ggggggcgag ccgttacccg
     1561 ctttaaagct gggttcgttc gataagatta tcgcggggag cgatctttcc cgggtaaaag
     1621 gtgatgaaat caccctgggc agcggtctgg cgaaaacgtt gaatgcgaaa tatgacgact
     1681 ggctggatgt gatggtggtc aacacggcgg gcgggcaggg cgcattgtcg ctaaaaatgc
     1741 gtggcatttt tgagtcaggc attaaagatt atgacgatgt cgcgatgaag atcccgctgg
     1801 acaccgcgca gcgcatgatg ggtacggatg gcgtcagcaa agtgcttatt ctgcttaaag
     1861 aggatgacac cgcggcgttt agcgcaaagc tgcgtcaatt tatcgaacgc aatcatttgc
     1921 cgctggtggt caaagactgg cgtgatgttt cactctttta tcaacaggtt gaagggttgc
     1981 tatcggggat ctatttcttc atcaagctga ttgttgcgct gattgtcatc ttcatgatcg
     2041 gcaactcgat ggcaatgaac attgttgagc gcacccgtga aattaccact ctgcgtgcca
     2101 taggcctgaa gccgcttcac gtcacgcgct tgtttctgac agaaggcatt tttattggtg
     2161 tgattggcgc agtcggcagc ctgctggtgg ggggcgtgtt agcctggatt attaatcttt
     2221 atggtattgc gatgccgcca tcgccgggac aaaccatcgg ctataccgcc tttataaaaa
     2281 ccagtaaccc ggaattgatt tgggtgacgg tggtattacc tattttgaca gcaacaggtg
     2341 cctctgtgtt accggcttta cgtgcttccc ggctgaatat ttctgacgca tttaaattct
     2401 catagggcta attatgaata tggcttttta cggcaagtgg tttgcttgcc tgtggctcgc
     2461 tacgtcgtgc gttcaggcgg cgagcacaga taataaggcg ctggagataa ttcgtcgcgc
     2521 tgacgaaatt cgttcgccga ataaaccgtt tcgttacacg ctgacggtaa cggaatataa
     2581 agcgggcgca acccagccag agaataaaca ggtcctcgat atttccatgc gctttatgaa
     2641 accgcagggt aatgaaaaag cggatgcacg ctcgctggtg cgttttattt atccgccgcg
     2701 agataaagga aaaatcatgc tttccgactg gtacgattta tggttttaca cgccggaact
     2761 gcgacgtcct atgcctattt cacggcaaca aagattaatt gggcaaatct ctaatggcga
     2821 tgtcattgtc actaactttg aatatgccta tgactcgacc ctgatggggg aagtcacgtg
     2881 tgctgaaaag cagtgttaca agctggcgct ggtgcgtaaa tcggcggata tcacctggcc
     2941 gaaggttatc tattacgttg aaaaagacgg tgataatcgc ccgtggaagg cggcctatta
     3001 ttcgcaggat gaccagttaa taaaagaagt gttgtatcag gatttccagc cggtgttggg
     3061 gaaaacgcga cccatgaaaa taaccgttac cgatgttcgt cacggcaata actattcagt
     3121 gatggagtac agcgatgttc gtctggaatc gctgccggag tttcatttca ccaaagagta
     3181 tattcagcga ggtgcgaaat gaaacgtacg ctgaagatct cttcgttgct gtgtgttgcg
     3241 ctgccgttga cggtacaggc ggattgcctg tcaggtgacg aggtggcaca aaatagcgat
     3301 gtgacgcttt acgagcaggt gtcttatgtc aataaacagg cttcggcctg gcagattgca
     3361 ggcaaaaatc cctacacccg tcacaacggt tatcaggagg cggggattgg catcaatagc
     3421 ggttgttcga taatcgacaa tacgctggac cttaagctca atctgtatgg aataaacgaa
     3481 tatgcgctta aacccgcagg gaaattcgaa acggatgata gccgcacgcg tgcgttaatt
     3541 aaccgcctca gtctggtcta tagcgcctcg gacagcgttc aatttgaagc aggcaaattt
     3601 gccgcgccgt caggaatgtt ctttttgcgt tcgccttctg atttgcaaac gcattattac
     3661 accggattcc agtcgacgcg tttgcacgat cccaaaatga catcggcgta tcaggcgtca
     3721 tcgtggggcg caaaaatgag cgtggataca cgcgattatg cgttttcggc gagtgtcata
     3781 ccgaagttag ccaccatcga caaacgctat gtgacgtctg gcaactggtc ggctaatcag
     3841 cagggaaaca gcgacgaagc ctatctgctg agcttcagcg atcatcgctt tggtgagcac
     3901 acgccgacag tcaatgtgcg gctcggacca tcgccgtcac tcgcgctgtc tgacagttat
     3961 cactacaccc cgcagcttac gctgaatgtc gacgcagcct accaccgctc ccagcaatgg
     4021 cggcatcttt cccaccgtga gacggcgcag gtcgaggaat atcagtttcc ggattcgctc
     4081 tatgaaacga aagatgaaag cggcgtagag ctggcgctgg gtgggcagta caccagtgat
     4141 aatttcagcg tgtttggcgt tgagtattac tttcagagcg agggttattc gcgcgctgaa
     4201 cagcgtcagc agcgcgagtt aattgacttc ctcaatacca caaccggcta tgcgccactg
     4261 gatcaggcat ttgattctta caaatacctg atggcttcag aaatcagcaa taccgccaat
     4321 caggggatgc tgcagggtaa gcactatctg aacgcctggg ccagtctgcc gctggcggga
     4381 gagtcgacgc tgcagccttc gctggtggtg aatctcgttg atggcagcac gttgctgggc
     4441 ctgcattatt ccacgccgct cagcgctata agtaaccagc ttgaagccta tgccggtggc
     4501 tatagcgcgc tgggaagtcg gtactccgaa tttgcgcttt ttggcgacac gttaggcctt
     4561 tatttgggct ttaaatacta tctataagga taaagagatg atgaataatg cttcacaacc
     4621 gacagtactg ctttttgccg gtcagggtaa tccggttatt ggtatgggca gcgatctttg
     4681 ggatctcaac gccagtacaa aacatatctg ggattgtgcc agcgatattt ccggactgaa
     4741 cctgcgtcgc ctgtgtcaga aagggccgat gaatcgcctt attcaaacga cggtacaaca
     4801 actggcggtg accgcaatta atgtaacgct ctataccctg tgccgcgagc gtcttgagaa
     4861 tttaacggtt gtgggctcct gcggacacag cgttggtgaa tacagtgcgc tctacgccgc
     4921 tggagctatt tctcttgaga cgctatttgg catgattcac ttcagagcca gcctgatgca
     4981 tgacgaaagc cagagaaata aaggcgcaat gctggcggtt aaaggcatca accgcgaaca
     5041 actgcagacg atgattacgg aatcaggcat tgcgctggat attagctgtg ataacacgcc
     5101 gcgccagcag gttattgggg gaacgcaggc tgcgttgaat gaatttgcca cgctgctgat
     5161 ggccgctgga tacgaaccgg ttaaactcgg tgtgagcggt gcctggcaca cgcgtttgat
     5221 ggaagacggc gttcaggcaa tgcgcgatta tcttgccgga ctggatatcg cttcgccaga
     5281 gcatcaggtt ctgatgaacg taacagcaaa aagcgaggtc gctccgtcaa ttatcaaaga
     5341 aaatctctca ttacacttaa ctcatacggt gaaatggact gaatctctcg atacatttct
     5401 gaatatgcca acctcggtag ctttccttga aataagtaat aagccctatt tggggaatat
     5461 gttcaacgat ttcgctggcg tggatcagca acgagttatg cattgtcgta aagcatttag
     5521 tgatgcgaag gtattcaaat gattcctgat tatttaactt ttattcgctt tcaggataaa
     5581 cgaagtctga tatacattta tgctattgga cttattctga taggctttta ttggaaaaat
     5641 gcaggattta cttttccatc agaggatatt ggtgtcgtta gtgggattct ggccctggtg
     5701 ctgtataatt ttatttttga tctcaaggcg tactgggctt ataaatgcgt cacgaagaat
     5761 atcgattttt cgtggtttaa gaaaaagcag aaccacaaaa tagaattatt tcttacgcaa
     5821 cctctggtgg caggatttct gtcgttaatc atgttgagtg caatgagttg ggggctatac
     5881 cagcgtctac cctcgttata tgcgctgttc ctgatttcgt tacttgggcc gttggttatc
     5941 tttctgctgt ttcggatgat ccgcaccagt tatgtcaagc aggtcgctat ttcagtagcg
     6001 aaaaaagtaa aatataaaag tctgactcgc tatgtgctgc tttcggtgtg catctcaacg
     6061 gttgttaacc tgcttactat cagcccgttg cgtaacagtg attcttttgt gatagagggg
     6121 cagtggttaa cgtttaaatc gataattgca ttgctcattc tttgtggcgt agtgttggcg
     6181 attaatctgt tttttctgcg cttctccagg cggtacgctt ttttgggcag gctttttttg
     6241 caggaaatcg ctctcttttt ctccagtgaa aatgcattgt cgaccttttt tgccaagccg
     6301 ctttggcttc ggttattcat attgctggtt attgaagtga tgtggattac gctggtgtcg
     6361 gtattggcaa cgcttgtgga atggcggatt tggtttgaag cctatttttt actctgctat
     6421 gtaccgtgct taatttacta ttttttctat tgtcgattcc tctggcataa cgattttatg
     6481 atggcatgtg acatgtattt ccgttggggg cattttaata agtgaggaag tgataggaag
     6541 tgaccagata atacatatat gttctgtact ctcttgcgca ttttgattgt tgactgagca
     6601 accagacagt tgatgtgcac gatttcccct cgcccaaaca gacgtgggcg ggggcacccg
     6661 gtgtgtgcaa tatacgaaaa aaaagcccgt actttcgtac gagctcttct ttaaatatgg
     6721 cggtgagggg gaccgcctga aataaatctg acttaatcca ggggtgttat ataatttcat
     6781 tataaacaat gtattacact ttttgttgtc cgatattatc taatgtattc ttagtgaagc
     6841 gagagactaa tggcatactg gatggcatac cagagtgacg ggaattagcg tatggcagta
     6901 ttgacggata cgaaagcaag acatatcaaa cctgatgaca aaccattgcc ccatggggga
     6961 attaccggac tgacccttca tccttcttca gtaaaggggc gggggaaatg ggtttttcgt
     7021 tatgtaagtc cggtgacaca aaaaagacgt aatgctggat tgggaactta cccagaggtc
     7081 agtattgctg aagctgcacg tactgcccgg ataatgcgag agcaacttgc tgcaggtgat
     7141 gatcctctgg agattaaaaa ggctgaatct gagaaagtcg ctatcccaac atttgccgat
     7201 gcagccaggc gtgtacatgc agaactgtct cctggatggg aaaatccaaa gcatgtaagg
     7261 cagtggttat cgacgcttga gaattacgcg tttcctcaac tgggagcaaa aacgctggat
     7321 tcgattacgg ctgcggacgt ggcagaaaca ctgcgtccag tctggttaac cttgtcagaa
     7381 acggcaagcc gggttaaaca gcgcattcat gttgttatgc agtggggatg ggcgcacggt
     7441 ttttgtgtag caaatcctgt tgatgtggtt gaccatttgc ttcctcagca gacaagagga
     7501 cgtgatgaac accaacccgc aatgccctgg aggcagttac cgctttttgt ggcgaccagt
     7561 gtgtataccg atgaacctta taatgttacc cgcgcactgt tattaatggt gatacttaca
     7621 gcaactcgct cgggcgaagc caggggaatg cgctgggctg aaattgattt tcataagcgg
     7681 gtatggacta tacctgcaga aagaatgaaa gccaggatac agcatcgtgt tcctttatcc
     7741 cggcaggcta tttacattct ggaaaatata cgtggcctgc atgatgaact ggtgttccct
     7801 tcacccagaa agcagcagat cctttccgat atggtgttga caagttttct gcgtaaaaag
     7861 aaagccgtca gtgacattcc ggggcgagtt gccacggcac atggttttcg ctcaacattc
     7921 agggactggt gtagcgaaca ggggtattcg cgggatctgg cggaaagggc gctcgctcat
     7981 acgctgaaaa ataaggttga ggcggcatat catcgtactg atctactgga gcagcgtgta
     8041 ccgatgatgc aggcatgggc ggattatgtg atgtctcaaa ttgtgaataa ataatagtga
     8101 aaatgtatat tgtacgat
//
LOCUS       DBFIFM010000198         8090 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.198, whole genome shotgun sequence.
ACCESSION   DBFIFM010000198 DBFIFM010000000
VERSION     DBFIFM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.198"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            289..2526
                     /gene="nrfB"
                     /locus_tag="N7C74_003913"
     CDS             289..2526
                     /gene="nrfB"
                     /locus_tag="N7C74_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HGU2433694.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2513..5485
                     /gene="nfrA"
                     /locus_tag="N7C74_003914"
     CDS             2513..5485
                     /gene="nfrA"
                     /locus_tag="N7C74_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HGU2433695.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSITTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQESANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARISQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAARQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRFFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5486..6376
                     /locus_tag="N7C74_003915"
     CDS             5486..6376
                     /locus_tag="N7C74_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HGU2433696.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYFSAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            6559..7320
                     /gene="envY"
                     /locus_tag="N7C74_003916"
     CDS             6559..7320
                     /gene="envY"
                     /locus_tag="N7C74_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HGU2433697.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(7366..7442)
                     /locus_tag="N7C74_003917"
     tRNA            complement(7366..7442)
                     /locus_tag="N7C74_003917"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7406..7408),aa:Arg,seq:tct)
     gene            7685..>8090
                     /gene="fimZ"
                     /locus_tag="N7C74_003918"
     CDS             7685..>8090
                     /gene="fimZ"
                     /locus_tag="N7C74_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HGU2433698.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYI"
BASE COUNT         1997 a         2103 c         2069 g         1921 t
ORIGIN      
        1 ttgcggggag cgggctttcg taatcttgcc ctgcctgctc acctccaggc acttccacta
       61 catcatgacc tgatcggtgc caccactcct cttcactatg acgttaacct cccctccttg
      121 acaccacgct tttgtctaca ttctacgcaa acggcaactt ttataaccag tgtaaaaata
      181 acgtgccgca atagtcatac ctattaatgg taaaaagctg tcacaattca taaaaaacct
      241 taatatacgc caccctaaac ataaccagcg ttaatgtaag gtttttgtgt ggactggctt
      301 cttgatgttt ttgctacctg gctctatggc ttaaaagtaa tcgcgataac gttagcggtc
      361 atcatgttca tcagcgggct ggacgatttt tttattgatg tcgtctactg ggtacgccgc
      421 attaaacgca agttgagtgt ttatcgccgc tacccgcgaa tgagttaccg cgaactgtat
      481 aaaccagatg aaaaaccgtt agcgattatg gttccggcgt ggaatgaaac gggcgtcatc
      541 ggcaatatgg ccgagctggc ggcgaccacg ctcgactacg aaaactatca tatctttgtt
      601 ggcacctacc ccaacgaccc ggacactcag cgtgatgttg acgaagtgtg cgctcgcttc
      661 ccgaatgtgc ataaggtagt ctgcgcgcgt cctggcccca ccagcaaagc cgactgtctg
      721 aacaacgtgc tggacgccat cacccagttt gagcgtagcg ccaatttcgc ttttgctggt
      781 tttattctgc atgacgccga agatgtgatt tcaccgatgg aattgcgtct gttcaactat
      841 ctggtcgagc gtaaagatct gattcagatc ccggtgtatc cgtttgaacg cgaatggacg
      901 cacttcacca gcatgactta cattgatgag ttttcagagc tgcatggcaa agatgttccg
      961 gtgcgtgaag ccctcgccgg acaagtgccc agcgcaggcg tcggcacctg tttcagccgc
     1021 cgcgccgtga ccgctctgtt agctgacggt gacggtattg ctttcgacgt gcagagtctg
     1081 actgaagatt acgacattgg cttccgcctg aaagaaaaag gtatgacgga aatttttgtc
     1141 cgttttccgg tggtggacga agccaaagaa cgcgagcagc gtaaattttt acagcacgca
     1201 cggacgtcaa acatgatctg cgtgcgcgaa tatttccccg ataccttttc gactgcggtt
     1261 cgacaaaaat ctcgctggat catcggcatt gttttccagg gctttaaaac ccataaatgg
     1321 acctccagcc tgacgctgaa ctactttctc tggcgcgacc gcaaaggggc aatcagtaac
     1381 tttgtcagct tcctcgcaat gctggtgatg atccagcttt tgctgttgct ggcgtatgaa
     1441 agtttgtggc ccgatgcctg gcatttcctt tctattttta gcggcagcgc atggttaatg
     1501 accctgctgt ggctaaactt tggcttgatg gttaaccgca tcgtgcagcg ggtgattttc
     1561 gtcactggct actacggcct gacgcagggg ctactatctg tcctgcgtct tttctggggc
     1621 aacctgatta actttatggc caactggcgc gcgttaaaac aggtacttca acacggcgat
     1681 ccacgtcgcg tcgcgtggga taaaacaacg catgacttcc ccagcgtcac tggcgatacc
     1741 cgctcgttgc gcccgttagg tcaaattctg ctggaaaatc aggtcatcac tgaagaacag
     1801 ctcgatacag cactgcgtaa tcgcgtcgaa ggtctacgcc tgggcggttc aatgctgatg
     1861 caggggctga ttagcgccga gcagctggca caggcgctgg cagagcaaaa cggcgtggcg
     1921 tgggaatcca tcgatgcctg gcagatcccg tcctcgctga ttgccgaaat gccggcctcc
     1981 gtggcgctgc attatgcggt actgccgctg cgtctggaaa atgatgagtt aattgtcggc
     2041 agtgaagatg gtattgaccc ggtttcgctg gcggccctga cgcgtaaagt cggacgcaaa
     2101 gtgcgttatg tcattgttct gcggggacaa attgtcacgg ggttacgtca ctggtatgca
     2161 cgccgacgcg gtcacgatcc gcgggcaatg ttgtacaatg cggttcagca tcagtggctc
     2221 acggaacagc aggccggtga aatctggcgg caatatgtgc cgcatcagtt cctgttcgcc
     2281 gaaatactga ccacgctcgg tcatattaat cgttcagcaa ttaacgtgtt gttattgcgc
     2341 catgaacgca gttctctgcc gctcggcaag tttttggtca ccgaaggcgt tatcagccag
     2401 gaaacgttgg atcgcgtcct gacaattcaa cgcgaattac aagtttcgat gcaatcacta
     2461 ttactcaaag caggtttaaa cacagaacag gttgcgcaac tggagtccga aaatgaagga
     2521 gaataacctt aatcgcgtca tcggatggtc tggtttactg ctgacgtctt tattgagtac
     2581 cagcgcactc gcagacaata tcggcaccag cgcagaagag ctggggctga gcgattatcg
     2641 ccattttgtt atttatcccc gtctcgataa ggcgctgaag gcacagaaaa ataacgacga
     2701 agcaaccgcc atccgcgaat ttgaatatat acaccagcag gtgccggata atattccgct
     2761 gactttatac cttgcggaag cctatcgcca ttttggtcat gatgaccggg cacggctgtt
     2821 gcttgaggat caactgaaac gtcacccagg agatgcccga cttgagcgca gtctggcggc
     2881 tattccggtt gaagtgaaaa gcattacaac tgttgaagaa ctgcttgccc aacaaaaagc
     2941 gtgcgatgct gcgccgaccc tgcgttgtcg cagtgaagtc gggcagaatg ccctgcggct
     3001 ggcacagtta cctgtcgcca gagcgcaact gaacgacgcg acgtttgctg catcgccgga
     3061 aggaaaaacg ctgcgaaccg atctgctgca acgggcaatc tacctgaaac aatggtccca
     3121 ggcagatacg ctatacaatg aagcacgcca gcagaacaca ttaagcgcgg cagaacgccg
     3181 tcagtggttt gacgtgcttc ttgccgggca gctggacgat cggatcctgg cactgcaatc
     3241 acaggggatc ttcaccgatc ctcagtcata tattacttac gcgaccgcgc tggcttatcg
     3301 tggcgaaaaa gcacgcctcc agcattatct cattgaaaat aagccgctgt ttaccacgga
     3361 cgcacaagag aaaagttggc tctatctgtt atctaaatac agcgccaacc ccgttcaggc
     3421 attggcgaat tatacggtgc agtttgccga taaccgccag tatgttgttg gcgcgacgct
     3481 accggtgctg ttaaaagaag gtcagtacga cgcagcgcaa aaactgctcg ccaccctccc
     3541 cgccaatgaa atgcttgagg agcgttatgc tgtcagcgtg gcgacccgta acaaggctga
     3601 agctctgcgt ctggcacgct tgctgtatca gcaagaatcg gcaaatctta cccgcctgga
     3661 tcaactaacc tggcaactga tgcagaacga gcagtcacgg gaagctgccg atttattgct
     3721 gcaacgctat cctttccagg gcgatgcgcg tatcagccag actttaatgg cgcgactggc
     3781 gtctctgctg gaaagtcatc cttacctggc aacgccggcg aaggtggcga ttttatcgaa
     3841 acccttaccg ctggcggagc aacgtcagtg gcaaagtcag ttgccgggta ttgcagataa
     3901 ttgcccggca atagttcgct tgctgggcga tatgtcgcct tcctacgatg ccgccgcctg
     3961 gaaccgtctg gcaaagtgtt atcgggacac gctacccggt gtggcgttgt atgcatggct
     4021 tcaggccgaa caacgacaac cgaacgcctg gcaacatcgt gcggtagcct atcaggcgta
     4081 tcaggttgag gactacgcca ccgcactggc ggccaggcag aaaatcagtc ttcacgacat
     4141 gagcaatgag gatctgcttg ctgctgccaa taccgcccag gcggcaggaa atggtgcggc
     4201 tcgcgatcgc tggctacaac aggcagaaca acgtggactg ggaagcaatg ccctctactg
     4261 gtggctgcat gcgcaacgtt acattcctgg tcagccggaa ctcgcactga acgatctcac
     4321 gcgctcaatc aatattgcgc cttctgccaa cgcttacgtt gcgcgggcga caatttatcg
     4381 ccaacgtcat aatgtcccgg ccgcggtgag tgatttgcgc gccgcgctgg aactggaacc
     4441 gaataatagc aacacccagg cagcgcttgg ttacgccttg tgggatagcg gtgatatcgc
     4501 acagtcgcgg gaaatgctcg aacaggcgca taaagggcta ccggacgatc cggcactgat
     4561 ccgacaactg gcctatgtga accagcgtct ggatgacatg cctgcgacgc agcactacgc
     4621 ccggctggtg attgatgaca ttgataatca ggcgctgata accccactga ccccagagca
     4681 aaatcagcaa cgcttcaatt tccgccgtct gcatgaggag gtcggtcgcc gctggacgtt
     4741 cagtttcgat tcttccatcg gcttgcgttc cggggcaatg agtaccgcta acaataatgt
     4801 tggcggcgca gcgccaggga aaagctatcg tagctacggg caactggaag ccgaataccg
     4861 catcggacgc aatatgctgc tggaaggcga cctgctctca gtttatagcc gcttctttgc
     4921 cgataccgga gaaaacgggg tgatgatgcc ggtgaaaaat ccgatgtccg gcaccggtct
     4981 gcgctggaag ccgctgcgcg atcagatctt tttcctcgcc gtcgaacagc agttgccgct
     5041 gaacgggcaa aatggcgcat ccgataccat gctgcgcgcc agcgcctcat tctttaatgg
     5101 cggcaaatac agcgacgaat ggcacccgaa cggttcaggc tggtttgccc aaaacctgta
     5161 cctcgatgcg gcgcaatata tccgccagga tattcaggcg tggacggcag attatcgcgt
     5221 cagctggcat cagaaggtag ctaacggaca gactattgag ccttacgctc acgttcagga
     5281 caacggctat cgtgataaag gcactcaggg cgcgcagctt ggcggtgtcg gggtccgctg
     5341 gaatatctgg accggcgaga cgcactacga cgcctggccg cacaaagtca gtctcggcgt
     5401 cgagtatcaa cataccttta aggcgattaa tcaacgtaac ggagagcgca acaacgcgtt
     5461 tctcaccatt ggagtgcact ggtaaatgcg taagttcatt ttcgtattgc tgacactgct
     5521 tttggtcagc cctttttcct ttgcgatgaa aggtattatc tggcaaccac aaaaccgtga
     5581 tagtcaggtt accgataccc agtggcaggg gctgatgagt cagttacgtt tgcaaggctt
     5641 cgataccctg gttttgcaat ggacccgtta cggcgatgca ttcacccagc cagaacagcg
     5701 cgcgttattg tttaagcggg ccgcagctgc gcaacaggct ggcctgaagc tgattgtcgg
     5761 gctgaacgcc gatccggaat tttttatgca ccagaaacag tcatccgcgg cgctggaaag
     5821 ctatcttaat cgcctgctgg ctgccgatct ccagcaagcc agattatgga gtgccgtacc
     5881 tggcgtaacg ccggatggct ggtacttcag cgctgaaatt gacgacctga actggcgcag
     5941 cgaagccgcc cgtcagcctt tgctaacatg gttaaacaac gcgcagcggc tgattagcga
     6001 tgtttcagca aaaccggttt atatcagtag ttttttcgcc ggaaacatgt cgccagatgg
     6061 ctatcgccaa ctgctggaac acgttaaagc aaccggcgtt aatgtctggg tacaggatgg
     6121 cagcggcgtg gataaactta ccgctgaaca gcgtgaacgt tatctacagg ccagcgccga
     6181 ttgccaaagc tccgccccag caagcggcat tgtttatgaa ctgtttgtcg ccggcaaagg
     6241 caaaaccttt acagcgaaac cgaaaccgga cgcagaaatt gcctcgctgt tagcgaaacg
     6301 ttcctcttgc ggtaaagaca ctctctattt ctctctgcgc tatttgcccg tcgcgcacgg
     6361 cattctcgag tattaaatct cctccaggta agtcgggtac gacctggctt acctctttcg
     6421 ctctcgtcag aattccatca agattaattg ctaaaaagcg tgctaaagaa agaagatcgt
     6481 tatcggcgaa cgggccgatc actcatgcag gaagcataga tcctttgagc ggagtttctg
     6541 atatctgagg agtgcgaaat gcaattgagc agcagtgaac cttgcgtggt gatcctgacc
     6601 gaaaaagagg tagaggtaag cgtcaataac catgctacgt ttacccttcc gaaaaactac
     6661 ctggccgcct tcgcgtgcaa caataacgtc attgaactct caacgttaaa tcacgtatta
     6721 atcacccaca tcaaccgtaa catcatcaac gattatctgt tgtttttaaa taagaactta
     6781 acctgtgtaa agccctggtc gcggctggct accccggtta tcgcttgtca tagccgtaca
     6841 ccggaagtgt tccggctagc cgccaaccac agcaagcagc aacccagcaa accctgcgaa
     6901 gcggagttga cgcgcgcatt gctttttacc gtattgtcta actttcttga gcaatcgcgg
     6961 tttattgccc tactgatgta tatcttacgc agcagcgtcc gcgacagcgt ctgccgcatt
     7021 attcaaagcg atattcagca ttactggaat ctgcgaattg tcgccagttc gctatgttta
     7081 agccccagcc tgctcaaaaa gaaattaaaa aacgaaaata ccagctatag ccagattgtc
     7141 acagagtgtc gtatgcgtta cgccgtacag atgttattga tggataacaa aaatatcact
     7201 caggtggcgc aattatgtgg ctatagcagc acgtcgtact ttatctctgt ttttaaggcg
     7261 ttttacggcc tgacaccgtt gaattatctc gccaaacagc gacaaaaagt gatgtggtga
     7321 agggcaaagc ggaaacggat aagacgggca taaataaaga agaaatggcg cgccctgcag
     7381 gattcgaacc tgcggcccac gacttagaag gtcgttgctc tatccaactg agctaagggc
     7441 gcgttgatac cgcaatgcgg tgtaatcgcg tgaattatac ggtcaaccct tgctgagtca
     7501 atggcttttg atctggttgc tgaacaagtg aacgaccgcg tctgattttc tgatttattt
     7561 cgctatagcg gcaaacaaac gcacaccgct gcgcgtctga atcaagaaaa cccgtatttt
     7621 catgtatcaa agtacaattt accgccctaa cggaaaattg tccgctccta tgagactggt
     7681 aactatgaaa ccaacgtcgg tgatcattat ggacactcat cctatcatca gaatgtctat
     7741 tgaagttctg ttgcaaaaaa acagtgaatt gcagattgtc ctgaaaacgg atgattaccg
     7801 cataaccatc gattatctcc gaacccgtcc tgttgattta atcattatgg atatagactt
     7861 gcccggaaca gacggtttta ccttcctgaa aaggatcaaa caaatccaga gcacagtgaa
     7921 agtgttattt ttatcatcga aatcagaatg cttttatgct ggcagagcga tacaagctgg
     7981 tgctaacggt tttgtcagta aatgcaatga tcagaatgat atttttcatg ccgttcagat
     8041 gatcctctcc ggatacacgt tttttcccag cgaaacgctt aactatataa
//
LOCUS       DBFIFM010000199         7997 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.199, whole genome shotgun sequence.
ACCESSION   DBFIFM010000199 DBFIFM010000000
VERSION     DBFIFM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.199"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..386)
                     /gene="gpU"
                     /locus_tag="N7C74_003919"
     CDS             complement(<1..386)
                     /gene="gpU"
                     /locus_tag="N7C74_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HGU2433699.1"
                     /translation="MKHREIRAAVLSALKENISERVSWFDGRPVFIDEQELPAVAVYL
                     TDASAADEFVDEGTWEATLHIEVFLRAKEPDSALDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEFMMWGSADLLWKIT"
     gene            complement(399..1022)
                     /locus_tag="N7C74_003920"
     CDS             complement(399..1022)
                     /locus_tag="N7C74_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2433700.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRASATTLNRVAGAIIAKTASSV
                     ARELAVPRRLIRARIRLSPARPDKVYAKVYINTGNLPAIKLGEARVRLSRRKRRKKGQ
                     RAALKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKITTAGPLAETFERERDRMYREKLPAQMMKAMTHQLRLVLKRK"
     gene            complement(1025..1306)
                     /locus_tag="N7C74_003921"
     CDS             complement(1025..1306)
                     /locus_tag="N7C74_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HGU2433701.1"
                     /translation="MSDLFTRMCCRMDVATVRVMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            complement(1299..1625)
                     /locus_tag="N7C74_003922"
     CDS             complement(1299..1625)
                     /locus_tag="N7C74_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HGU2433702.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     EAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSATV
                     AVKINV"
     gene            complement(1713..3737)
                     /locus_tag="N7C74_003923"
     CDS             complement(1713..3737)
                     /locus_tag="N7C74_003923"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HGU2433703.1"
                     /translation="MPELNDSISATPKASMKNKSWFRMQAGGPGDADIYIYDEIGFWG
                     VTAKQFVSELNALGDITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASM
                     ASVIAMAGDTVIMPENAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRDTTVTIPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMF
                     GGRYQTLQAQCVADPDCSLEMARERLLNEMGKESSPTNKNTPAHIYAGNGNFVGDGIR
                     QAMLARAGFENVEKDNAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDF
                     GNILLDVSNKGLIQGWEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEY
                     KYITTSDRKETIALATYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKV
                     LTDNPKLSDGKALFHADHKNIATGGISVSGLDAARQMMRLQKEGDRALNIRPAFMLVP
                     VALETVANQTIKSASVKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTD
                     TIEVAWLDGVDTPYIDQQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
     gene            complement(3682..5184)
                     /locus_tag="N7C74_003924"
     CDS             complement(3682..5184)
                     /locus_tag="N7C74_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU2433704.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGIKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNP
                     DEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTESQHEE"
     gene            complement(5184..5396)
                     /locus_tag="N7C74_003925"
     CDS             complement(5184..5396)
                     /locus_tag="N7C74_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433705.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVIFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            complement(5393..7516)
                     /locus_tag="N7C74_003926"
     CDS             complement(5393..7516)
                     /locus_tag="N7C74_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGU2433706.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPSRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVESSPGRDI
                     TDTKWRCGGAHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDF
                     VAAGQAARLMCPHCRGLIAPEQKRELNNQGIWLREGERAAADGSITGTPRNSRIASFW
                     MEGPAAAFQTWEQLIFKLLAAEEEYERTGSEETLKAVVNTDIGRPYLPRSATEQRKSE
                     LLEQRAEPFPRRSVPDGVRFIEATVDVQGGKNRRFVVQITGYGEQGERWIVDRYNIRH
                     SLRCSPNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLMAMAVDTGGEAG
                     VTDNAYRFWRRCRSDGLGNRVFLFKGDGLRRDRLINRTFPDNTGRSARRARASGDVAL
                     WLVQTDAFKDRVNNALWRDTPGPNYIHFPDWLGRWFYDELTYEERGSDGKWRKPGRGA
                     NEAFDLLVYADALAVLHGYEKIRWPSAPDWAQRETWLVFPQERSGETVSPELTAGAEK
                     RRRRKKKLRTERAEDNPWITSGGWL"
     gene            complement(7513..7989)
                     /locus_tag="N7C74_003927"
     CDS             complement(7513..7989)
                     /locus_tag="N7C74_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HGU2433707.1"
                     /translation="MDGELKNMKLNINQLAALSGLHRQTVAARMADVPLAPGSNEKKK
                     LYLLTDLITSLLEKPPSSEDEDMDPHARKAWYQSERERLKFQHETVQLVPVSDVRRSF
                     SVVVKAIVQVLETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
BASE COUNT         1716 a         2515 c         1914 g         1852 t
ORIGIN      
        1 gtaattttcc acaggagatc tgccgatccc cacatcataa actcatcatc ccggcggtag
       61 tcatacccct gaagattcat cttcagcagt aatgcactga gcccgggaac tgcctccagc
      121 gcaggaagaa ttttttcttc catccacata tccagtgccg agtccggttc ttttgccctg
      181 agaaaaacct caatatgcag tgtcgcctcc caggttccct catcaacgaa ctcgtcagca
      241 gcagacgcat ctgtcaggta aacagcaaca gcaggcagtt cctgttcatc aataaaaacc
      301 gggcgaccgt caaaccagct caccctctca gaaatatttt ctttcagggc agacagaact
      361 gccgcccgta tttcacggtg tttcatacac cctcccagtc attttctttt cagcaccagg
      421 cgtaactgat gcgtcatggc tttcatcatc tgcgccggta atttttcccg gtacatccgg
      481 tcccgttcac gttcaaaggt ttctgccagc ggtccggcag tcgtaatctt caccacttcg
      541 atcggcagac ggtggcgttt cggcctccct ttgctgtccg cgccggtgga tgatgatgcc
      601 cacggcatac gctgcatcac atgccagcgt ccgttagcca gccgggtgat aaaggcgtcc
      661 gggatccgtc ttttccccac aatcagcaca ctgccgcccc ctttcagggc cgcacgctgt
      721 cctttctttc tccgttttct gcgggaaagt cgaacgcggg cctcccccag tttgatggcg
      781 ggcaggttgc cggtattgat gtaaaccttt gcgtaaacct tatccggtcg tgccggactt
      841 aaccggatgc gggcacggat aagacgacgg ggaacggcca gctccctggc aactgaagag
      901 gccgttttcg caataatggc ccccgccact cggttcagtg tcgtggcaga ggcccgggga
      961 acggcacggc gatcaattgc atccagattt ttcatggcct gcgccagacc ttttattgcc
     1021 atactcattc ctgttcgaca aaaatccggg gtttaccgtt gtacgtgtca taacgggtca
     1081 ccgtcagtgt gcgccccgca aacacaacaa catcatgacg ggccggacgg taccgggctg
     1141 aaaacaccac cagtgacaac tggctgcccg aaagcgcccc catctccgcg gactcttcct
     1201 ccggcatcac gtcgtacacg acgccgttaa tctccgcctg tttgcccatc acccgaacgg
     1261 tcgccacgtc catccggcaa cacattcgcg taaacagatc agacattgat ttttaccgcc
     1321 acagtggcgc tgtttgcagg ggcattttcc caggctaccc ccgctgccac cgcaccgtct
     1381 gcagccagct gcacaacccc gtccttcaga taaaccaccg cgccggactg aatgtcgtca
     1441 gcagactgtt tgggcagcag gaacacgcct tcggcaaaac cgtcaccggc ctcaccggca
     1501 ggaatatcgg taatggccac ggccaccata ctgccgacca ccaccgcagc accgctcagg
     1561 atggtctgat ctccggcatt caccagttca atggtggtac cgtcctgtac aaaatttttc
     1621 gccataatgc tgtttctccg gacagcccct gtggggctgt ttttcaggca taaaaaaagc
     1681 cctttcgggc agtgattgtg ataacgcggt tatcaggcca ccgacgaacg caccagcccg
     1741 cgccagtcaa gtggtgccac tccggcatca atacggattt ttgtggcaat gccgtcagtg
     1801 gtgaaacctt cctgctgatc aatgtatggc gtgtccacac catccagcca ggccacttca
     1861 atggtgtcag tgccctgtgc cgccgccaga taccaggttt tcgggtctgc cgcatcaaga
     1921 cgcgcttctg caatcacctc agcaaagttc tggatagggt taatgacacc ggcgtttgca
     1981 tccgcccctt tcacactggc cgatttgatg gtctggttcg ccaccgtctc cagtgccacc
     2041 ggtaccagca taaaggccgg acggatattc agggcacgat cgccttcttt ctgcaggcgc
     2101 atcatctgac gggccgcatc cagtccggaa acggagatcc ccccggtggc aatatttttg
     2161 tgatcggcat ggaacagcgc cttaccgtcg gacagtttcg ggttatccgt cagcaccttg
     2221 tagaccaggt caccaatcgt tgccttcgcc gcacgcccca tcttcatcgg cacgtccacc
     2281 agcatattca gatcatcatt gataatggcc tggcgggtga tggagaaaat ctccccgtaa
     2341 gtggccagtg caatggtctc cttgcgatct gaggtggtga tgtatttata ctccgccccc
     2401 tcacgaacct ggcgcagaga accaaaaccg cccatcccca cgcgatacgc tgttttgaag
     2461 tctgacaggc gtcccttacg ggtccacttc tggaaggttt cttctgattc ctcccagccc
     2521 tggatcagcc ccttgttcga cacatccagc agaatattgc caaaatcaga ggtgctgtgc
     2581 gtcagcgcca gcccgaccat ctgcatgggg ttataactgg ccaccccaat accgcgctcc
     2641 gtcagtgaca tgcgagccca ttcacgcagg gtcatcccgt tataggcgtt atccttctcg
     2701 acattttcaa atccggcacg ggccagcatc gcctggcgga tcccgtcccc cacaaaattg
     2761 ccgtttccgg cataaatatg ggccggtgta tttttgttgg tcggcgagga ctccttgccc
     2821 atttcattca gcagacgttc acgggccatt tccagcgaac agtcaggatc agccacgcac
     2881 tgtgcctgaa gcgtctgata gcgaccgccg aacatggcaa acagatcgtt aatgcctgac
     2941 atgcgggctt tctgctcagc cataacgcgg gcgcgaatgg tcgcctcatc agacactgcc
     3001 ggtaccggtg atggttctgt taccgccggt gcagggattg tcactgtggt atcacgcggg
     3061 gcactgttgc gtggcggagt aatcatgttt cggatggatt ccggcatctt tttaaattcc
     3121 tctgtacgtt ttgactgaat acatgccatt gcctcaacag cgggtgtcac ctggtcagca
     3181 aatccgtgtg ccagacattc ggcaccggac atccaggttt catccgccag catggcggca
     3241 atttcatcgg tggtttttcc ggttttctgc gcataggctg gcaacagtac cgattcgact
     3301 ttatccagca aatcggcata actgcgcata tcctcagcat ccccgccact gaatccccat
     3361 ggcttatgga tcatcatgaa ggcattttcc ggcataatga ccgtatcacc ggccatcgca
     3421 atcacagatg ccatcgaggc ggcaacgcca tccacataca cggtaatggt cgccccctga
     3481 tttttcaggg cattaaaaat ggcgatgcct tcaaagacat cgccacccgg tgaattgata
     3541 tggagattaa tgtgggtgat atcacccagt gcattcagtt cgctgacaaa ctgcttcgcg
     3601 gtaactcccc agaaaccaat ctcgtcataa atataaatat ccgcgtcacc cggcccccca
     3661 gcttgcatcc tgaaccagga tttattcttc atgctggctt tcggtgtcgc gctgatactg
     3721 tcgttcagtt ccggcactgt tgcctccttt gtcgttgacg gggtcagtat caaagaccag
     3781 ccccagtctg ctgttttcat caatttcagc cttgcggcga cgtttgacct catccggatt
     3841 gcgcccaccg gcacgcaccc agtcagattc tgtcgctgca ccaccccgga tctgaattct
     3901 ccaggcttca gcttccttaa ccgggtcgat ccacggcatc accggaccgg aatacgtcgc
     3961 gttatatagc gttttcatct ccacatccgc cggaattttc agcagacctg ccgcaaccac
     4021 catattcagc catgtccggt acaccgggcg ggttaccgcg ccaataaaac agtcctgcag
     4081 gatcaggtaa ccatccgtgg actcgaccag ctcctgccgc tgggcgctgt aggtgccgtt
     4141 atagttacgc gccgcactgg aaaaactcag acgactgcct gctgccactg cacgcaactg
     4201 gccgttgcgg aaagtttcaa ggttgggatt gggacggtca gatttgacca tgccgatatc
     4261 ctcgcccttg cgcaaatcgt cataaataat acccggggtg atatggactt cccgctcggt
     4321 ctctttgatc cccggatctt catagtcctg tccgtcacct ttacggatat acagtcccag
     4381 cgccgcagca atacgcgccg ctgtcagttc cgcatcctca tactccttaa gggcactgat
     4441 ccgcatcagc acccccgata acatggatga gcctcgcgtc tgatgcagac gacgagtgaa
     4501 cttcaggtgg atcatttttc cggcagcgat ttctttcgta tcactctgcc ggccgctgac
     4561 cggataattt ttataaacca gatatttttt cggtcttccc cactcatcaa gaaaaacccc
     4621 ctgattcagt ccggcggatt catcagtgcg catgggaaca aaatccggct ccatcgcctc
     4681 aagccagaat ggcactcccg ccgtccgttc cagaccgttt cccgcaccac tgaccatctg
     4741 cgcaaacact tcaccatccc gcagccaggt ccgcagcagt aaacgttcaa gcacaggacg
     4801 ggtatactgc cctgtcacat ccggactcac ggaccattca gcccacaaac ggcggatatc
     4861 cgcagccagc tcagccgcca tttccccgtt ttttcgtaat ggctgaggct ccacaataat
     4921 tcccctggca ccaatcaccc gctcttccag cttgtcaaac acaccaatca ccaggtcatg
     4981 attgatatcc agaaaacggg cctgctcccg cagggaaacc gcaccgtatt tactgagctg
     5041 atcagcagag cgattttccc gccgggcttt atgtgtccgg gtcggtttca ccgcctcata
     5101 ggccatgatt aacgcccttg aacgcagtct ggctgctttc cacccggggg aaaacacgcc
     5161 gatcacatca tcaataattg ccattaaaac ctcgccagtt taaatcccgg ttttccccgc
     5221 ctgcggctca ccatcgcggc aagcctgcgt tcccactcct gacgtccggc gcggatctga
     5281 gaaaggcttt ccagcgtcag ttgctgcccg ttgaagatga cagactttcc ctccagtacg
     5341 gccatttccg cttcacggta ccgctgtatc atttctctgg cttcttctgt gctcacaacc
     5401 agcctcctga tgttatccat ggattatctt ccgcacgctc cgtccgcagt tttttcttcc
     5461 ggcgacggcg tttttctgcc ccggccgtca gttccgggga taccgtttca ccagaacgct
     5521 cctgcgggaa gacgagccac gtttcccgct gtgcccagtc cggtgcggag ggccagcgga
     5581 tcttttcgta accatgcaga acggcaagcg catccgcata aaccagcagg tcaaacgctt
     5641 cgttagcgcc cctgcccggt tttcgccatt ttccgtcact gccgcgctct tcataggtca
     5701 gctcatcgta aaaccaccgc cccagccagt cgggaaagtg gatatagttc ggccctggtg
     5761 tgtcacgcca cagggcatta tttacacgat ccttaaacgc atccgtctga accagccaca
     5821 gcgcgacatc gccactggct ctggcacggc gggcacttct gccggtatta tccgggaagg
     5881 ttcggttaat cagcctgtca cggcgaagtc catccccctt gaacagaaac accctgttgc
     5941 ccagtccgtc actccggcaa cgacgccaga aacgataggc gttatctgtc accccggctt
     6001 cccctcccgt atccaccgcc atggccatca gacgcatgcg cacatccgga tcagaagcca
     6061 gcggccatgt tttatggaac acatccgtca gcaacaaatc ccagtcctcc ggatatgccg
     6121 ccggatcaac cggcagactt tcaccgttgg gactgcagcg cagtgaatgc cggatgttgt
     6181 agcgatcaac aatccagcgt tccccctgct ctccgtatcc ggtgatctgc acaacaaaac
     6241 ggcgattttt accgccctgt acgtcaaccg ttgcctcaat aaaacgcaca ccatccggca
     6301 cagatcgccg gggaaacggc tcggcacgct gttcaagcag ttcactttta cgctgttccg
     6361 tggctgaacg gggcagatag ggtcgtccga tatcggtgtt caccaccgct ttcagggtct
     6421 cttcactgcc ggttcgctca tactcttctt ctgccgccag cagtttaaaa atcagttgtt
     6481 cccaggtctg aaacgccgca gctggcccct ccatccaaaa tgacgcaatc cgggagtttc
     6541 gtggcgttcc ggtgatactg ccgtccgccg ccgcccgttc accttcacga agccagatcc
     6601 cctggttatt cagttcgcgt ttctgctcag gggcaatcag cccgcgacaa tgcggacaca
     6661 tcagacgggc agcctgaccg gcagccacaa aatccgggtt attccggtat ccggtcatgt
     6721 tatccatcac cggctgaaaa tattccccgc agtgcggaca cggccagtac caccggcggc
     6781 ggtctccccg gttatacagt gacaggatcc ccgttgttgg cggtgcctca tgtgcgccac
     6841 cacaacgcca tttggtatcg gtgatatccc gccccggtga actctcgacc agggtcatcc
     6901 ccgaggacat aaaggtggtg gtacgctttg aggccagcgt gaaggcatcc ccttccccgt
     6961 ccacattttc agggaaacgg tcataatccg tcagcgccac acgacggtaa tccgaagagg
     7021 aaaagacggt gatcgacggc cagccaatct tcaggaagga gccgtcaaga aacattttat
     7081 cgtggacgtt gttgtcatta cgggaaggac tgaggcgctt gctgacctcc ggactgtggc
     7141 gaaacgtcct ggaaagacgc gttctggaat gctcacgcgc cttcgtctca gtcatctgca
     7201 ccaccagcat atccgccgga tcacagatga tgccgtacac aatccagcca tcaatcagcc
     7261 cttcggtttt cccggttcgc gcaggtccca caaacaccac cgcgtcatat tcacgggctg
     7321 ataatgtatt aatggggtca atcatatagg gcgtcagcga tgactcccac ggaccggaag
     7381 tattggctcc ccgtggaacc cgcatataac gcctgatggc ttccgctact ggtaaccggc
     7441 tgggtgggcg aaacagcgag gccacttcgc gccagatatc ggatgcgcgg ctatggctct
     7501 cgttcacctg attcacatat cggcctcatc acaacagtca atgactgcct tttccagtgt
     7561 gtcgcggatc tcatcaacca caatctgtac ttcattcagt tgtgatgcag tccaccccct
     7621 gtccctctcc agccggtcag gccaggtttc cagtacctga actatcgctt tcaccacgac
     7681 agaaaaggac cgcctgacat cactgactgg cacaagctga acagtttcat gctgaaattt
     7741 aagacgctcg cgctcggact gataccatgc cttacgagcg tgaggatcca tatcctcatc
     7801 ttcggaagat ggtggttttt ccagcaacga agttatcaaa tccgtcagga gatacagttt
     7861 tttcttttca ttactgcctg gtgcaagagg aacatccgcc attctggcgg caacagtctg
     7921 ccggtgcaga cctgaaaggg ctgccagttg attaatattt aacttcatat ttttcagctc
     7981 gccgtccatt tacatcc
//
LOCUS       DBFIFM010000200         7990 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.200, whole genome shotgun sequence.
ACCESSION   DBFIFM010000200 DBFIFM010000000
VERSION     DBFIFM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.200"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(178..1035)
                     /gene="murI"
                     /locus_tag="N7C74_003928"
     CDS             complement(178..1035)
                     /gene="murI"
                     /locus_tag="N7C74_003928"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HGU2433708.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(980..2824)
                     /gene="btuB"
                     /locus_tag="N7C74_003929"
     CDS             complement(980..2824)
                     /gene="btuB"
                     /locus_tag="N7C74_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HGU2433709.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGGSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQPDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNIIIGHGNVGAG
                     VDWQKQSTAPGTAYVKDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     regulatory      complement(2889..3079)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3193..4293
                     /gene="trmA"
                     /locus_tag="N7C74_003930"
     CDS             3193..4293
                     /gene="trmA"
                     /locus_tag="N7C74_003930"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HGU2433710.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECSVLLTAK"
     gene            complement(4333..4692)
                     /gene="yijD"
                     /locus_tag="N7C74_003931"
     CDS             complement(4333..4692)
                     /gene="yijD"
                     /locus_tag="N7C74_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HGU2433711.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4692..5342)
                     /gene="fabR"
                     /locus_tag="N7C74_003932"
     CDS             complement(4692..5342)
                     /gene="fabR"
                     /locus_tag="N7C74_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HGU2433712.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            complement(5446..6762)
                     /locus_tag="N7C74_003933"
     CDS             complement(5446..6762)
                     /locus_tag="N7C74_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate-proton symporter"
                     /protein_id="HGU2433713.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADKNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPVGCWLR
                     LSLENDVPEPAHNKKAAASESAFSLLMQHKATIANGILLAIGSTVATYISLFYYGTWA
                     AKYLGMNQNYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILISRVMVLICSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            complement(6805..7971)
                     /locus_tag="N7C74_003934"
     CDS             complement(6805..7971)
                     /locus_tag="N7C74_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HGU2433714.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGIVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDHFPCDVIFGM
                     HNMPGLPVGKFYFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
BASE COUNT         2087 a         2176 c         1895 g         1832 t
ORIGIN      
        1 gctacagagt caagcattta tttttgcttt ctctgtcgga attctcagga gaaccccgct
       61 gacccggcgg cgtgtttgcc gttgttccgt gtcagtggtg gcgcattata gggagttatt
      121 ccggcctgac aagaggaaat ttaaaataat tttccgaccg cgcaacattc aaccaaatca
      181 gcctaaaact gccagttttt cgagcgtttc gaagccgtaa cgctgtaaaa cgggcaataa
      241 ttgttcagct tctggcgtca ttgccataca aaaggcaata ttcgcatcgg cagattttgc
      301 atccggggct tcatgttcta acaaccaggc cgttcggcga gcaatcgctg cgccagaatc
      361 caccagccgg gttccctctg gcagcacttg taacagttct tcttgtagta gagggaaatg
      421 ggtgcaaccc aatacaacgg tatctggcgg ctctttcatt cttaaccacg ggcgtaggat
      481 acgttttagt gcatccagag aaacatcttc gccatgcagc ttcgcttcag ccaactcaac
      541 catctctgcc gagcccagca tttctatctg gcattcatta gcgaaacgcg cgatcagctc
      601 atgagtataa gaacgtttaa ctgttccgcg ggttgccagt aatccgacaa tgccatttgc
      661 cgtcagacgt gcagcaggtt taatcgccgg cacgacacca acaaccggga agtcgaactt
      721 ttcgcgtaat gcaggaagtg aaacggtact ggcagtgttg caagcgacca cagccagcgc
      781 aaggggataa cgttcttgca ccgcggtgac aattgccacc actcgctcaa caataaacgc
      841 ttcgcttttt tcgccatacg ggaaagcgac gttatcgaaa gcataaatgt aatggagatc
      901 cggtaagaga tgccggatct cgtcatagac cgacaaccca ccgacgccgg agtcaaacac
      961 cagcacggtg ggacgtggtt cagaaggtgt agctgccaga caaggtgtat tcccgtcctg
     1021 cagtttggta gccatagact gtctcataat ctttgtcgaa caggttggct attttaccac
     1081 gaactgtcag gtgagaggtg accggatacg caaccgcaag atcccacaag ctcacaccgc
     1141 ccattttaac ggtttgataa ggataagatg agtaatcctt atcatagcga gtgcctaaat
     1201 actgataagt aataccccag tcgaagtcat acaactgcca gtcgagctgg tatttcacct
     1261 gctgtttagc acggcgtaac aacggcgtgt cggtaattgc attgcgcgca tcgacataat
     1321 cataactcac agtatgcgtc agtggtccgg tatcaaaatt ggcggtcgcc tcgacgccct
     1381 taatccgcgc tttcccttcg ttgtaatatt tcagggtgtg atcatcataa tcgatcaagt
     1441 cactgacatc gttacgatat ccggaaatac gccagttcac cccagcggtt aagccttcaa
     1501 acgcgccttc ccactgtttg cttttctccg ggtccagatt cggatttccg tagaagccat
     1561 acagttgccc cagatttggt gccttataag atgtcccgta ggaagcaatg aagcgataac
     1621 cttcgatgaa ttcccaaccg gcgctggttt gccaggttcc atgacgacca aactgtgagt
     1681 tatcgtcgct gcgtgctgcg ccttcaaagg taaaatcgcc gacttgttgc agcccggtca
     1741 gatagatgcc ggtattacgt tgatcatatc catccttaac ataagctgtg cccggtgccg
     1801 tgctctgctt ctgccagtca acacccgcac caacattacc gtggccaatg atgatgttgt
     1861 ttgcccactg gacggtgtat tgcttcatct catcgagcgt cgccgacgaa tcataacgac
     1921 cataatgggg atcgtagttg taatctttgc tatggctata gctggtaatg agttgtgatt
     1981 taatcagttc gccgttatat cgcagcccgg cgtcccaact ttgactatag agtttacggg
     2041 tatcgaccaa tggtgaaccc ggagaataat acgcgtcata attggtacgg ttatcatagc
     2101 catagccgcg cacaaagccg ctccaggcat cagtaaagtt atgctccagc gcgccataaa
     2161 gcgttttact taaaaaacca tcgttatctg gctgcgcttg cgttccggta ttaccatagg
     2221 caacaacatc ataaccatga gtatgggcat aatcgcccaa caacgttact cgtgtcttat
     2281 cccccagttg ttgctgtgta gagacatcat agttttgata actattgctt ccccaccctg
     2341 ctgaaatttc cgttccgggt tcatcgcgcg tcgtgatgat attcaccacc ccacctattg
     2401 catcggaacc ataaacggcg gagcgtggcc cacggatata ttcaacacgc tggacaagcg
     2461 caatagggaa ctggctaagg tcggcagaac cactcccccc cgccagattc aggcgtacgc
     2521 catcaattaa caccaacaca tgactggcat ttgtaccgcg aataaaaata gatgagagct
     2581 gacctgaacc gccgttttgg gtgatatcga cgcccggaag acggcgcagc acatcattaa
     2641 ccgaggtcga ctgccagcgg tcgatatcct gacgcgtcac aacggtggtt ggtgcaagca
     2701 cagtgctgcg cggctgttca aaacggttag cagtaacgac gagagtatcc gggctggtat
     2761 cctgtgccca agcggaaaag gctgtgacag aacacgccgt cagcagcgaa gcttttttaa
     2821 tcattgtaaa gcatccacaa tagaagaagg atgccgcagg tttcatcaat attacgcgat
     2881 gatgagaacc agatgcgacg ttggccggca ggtcttcggg cttggagggg tatctaagat
     2941 actaagagat gatgacttcc caccgaatgg cagtgtccgc ataacgcaat catcgcacct
     3001 ttccttaccg ctgcgcgtca gctccagatt cgcactggat tccctattaa ctcacaggac
     3061 cggcaagtgg atgctacagg ttgtaacaag ttactgtcca gacgtagctc acaaatagga
     3121 attcatcaag atctggacat ctgatgagca atccctacaa tcgccgcgta ctttaatttt
     3181 tcaggataca tcatgacccc cgaacacctt ccaacagaac agtatgaagc gcagttagcc
     3241 gaaaaagtgg tacgtttgca aagtatgatg gcaccgtttt ctgacctggt tccggaagtg
     3301 tttcgctcgc cggtcagtca ttaccggatg cgtgcggagt tccgcatctg gcacgatggc
     3361 gatgacctgt accacatcat tttcgatcaa caaaccaaaa gccgtatccg ggtggatagc
     3421 ttccccgccg ccagtgaact catcaaccag ttgatgacgg cgatgattgc gggtgtgcgt
     3481 aataatcccg ttctgcgcca caagttgttc cagattgatt acctcaccac gctgagtaat
     3541 caggcggtgg tttccctgct gtaccataaa aagctggatg atgagtggcg tcaggaagcg
     3601 gaagccctgc gcgatgcact gcgcgcgcag aatctgaatg tgcatctgat tggtcgggca
     3661 acgaaaacca aaatcgcgct ggatcaagat tacatcgacg agcgtctgcc ggtcgcaggg
     3721 aaagagatga tctatcgtca ggtagaaaac agctttaccc agccgaacgc ggcgatgaat
     3781 attcagatgc tggaatgggc gctggacgta accaaaggct caaaaggcga tttactggag
     3841 ctgtactgcg gcaacggtaa cttctcatta gcgctggcgc gcaattttga tcgggtatta
     3901 gccaccgaaa tcgccaagcc atcagtcgct gccgcgcaat acaacattgc cgctaaccat
     3961 attgataacg tacaaattat tcgtatggcg gcagaagaat ttactcaggc gatgaatggc
     4021 gtgcgcgagt ttaaccgcct gcaagggatc gacttaaaga gttatcagtg cgaaaccatt
     4081 tttgtcgacc cacctcgcag cggtctggac agtgaaaccg agaaaatggt gcaggcgtat
     4141 ccacgtattc tgtatatctc ctgcaatccg gaaacgttat gcaaaaatct ggaaacatta
     4201 agccagacgc acaaggtcga acgtctggct ctgtttgatc agttccccta cacgcaccat
     4261 atggagtgca gcgtattact gaccgcgaag taaaacccat gccggatgcg ccagcatccg
     4321 gcatgatacc gattactcag caacttcctg cttacggtta cgcatcttcg cgccaatcca
     4381 gaacaccata atgacggaga gtactgccgg gaagaagtta gagccgatat ccggatattc
     4441 cgcacgaact accgtgctgt acagcagtac gccgagaata aaacaggcag ctgccagacc
     4501 cggcaagcct accggcatgg tgcgattaag ataacgttga tgcaggcagt aaaccgtcag
     4561 caccagggaa ataatcggga atacagaaaa tggcacaatg gagctaaaca gcgctgcgaa
     4621 agtaccatta atcgataagc cagcaaccaa cgccagcagc agcgtacctc tatcttgatt
     4681 tgcttgtttc attactcgtc cttcacattt cccggaataa ttgcggtttt ctcttgttca
     4741 cggcgatacc agtaataagc ccctttcgaa atcattcgca gttgcagtac cagtcgctct
     4801 tctaattgcc gacgttgttc aacgccgacg tccaacgcct cggcacccgc actgaagaca
     4861 attgtcacca ttgcttcggc ttgcgcttca gtaaacgcac gcggcatatg gttttcgagt
     4921 tccagatagt ccgcaagttc cgcaatgaag tgctgaattt cacgcgcaac ggcggcacga
     4981 aacgcagcgg aggtgccgga gcgttcccgc aataataacc ggaaggcgtt aggattatta
     5041 ccgatgaact ccataaatgt ggagaccgag gtgcggatca cactcccgcc tttggcgata
     5101 cgctgacgcg cctggcgcat gagttggcgt agcattaaac cgctctcatc aaccatggtc
     5161 agacccagtt cgtctacgtc tcggaaatgc cgataaaaag aggtgggagc aatgcccgct
     5221 tcacgcgcca cttcacgcaa actcaggctg gcgaagctgc gttcagcact taattggcta
     5281 aatgcggctt ccaccagcga acggcgggtt ttttcttttt gttgcgctct tacgcccatc
     5341 acgatgtctg aatccttgcc aaaagtactg ctggcactat accagagaat gaacatactg
     5401 gattgctcca gtattgtgaa tgaacggtaa cgcaataaaa catatttact gcaattcttt
     5461 ggccggttct tttacgtaca acaacgccaa catcgacagc aacgtgcctc cacccagata
     5521 ccacgccgga gccgtcaaac tatccagcaa cacgatggat tgcgtagcga aatattgagc
     5581 aaaaccgcca aagattgcca caccaatgct ataaaccagc gcaaaaccca atgcccgaat
     5641 ccgtttcggc aacagttcag agatcaacaa catcactggc acaccgccaa gcgtggtaaa
     5701 gctgaccatc acgaaaacca ccgtcagcaa catgcctggg cttgggtaat tcaccaacag
     5761 ccagaatgac ggccagctac agatcagcac catcacacgg gagattaaaa tcagcttttt
     5821 acgcccgacc gaatcgcaca acatccccac cagcagcgca cccacaaacg taataacgcc
     5881 cgccagtaac atcgctgcat gagaatagtt ttggttcatt cctaaatact tcgccgccca
     5941 tgtaccgtaa taaaagaggg agatataggt tgcgactgta ctaccaatag cgagcaagat
     6001 accgttagcg atagtcgctt tatgttgcat aagtaaggag aaggcgcttt cgctggctgc
     6061 ggccttctta ttatgtgctg gctcaggaac atcattttcc agactcaaac gcaaccaaca
     6121 gccgacaggc gctaataaca cgccaataaa aaacggcaca cgccagcccc attcagccat
     6181 gacggtttct gaaccggtcg caaagggcag ccatgcactt aaacctaaag cgacaacacc
     6241 tccaaaagtt gttgccaggc cttgagtcgc cagtgaccaa ctgctgtaaa aaccgcgacg
     6301 atttgccgga gctgattcaa ccaacaaaga cattgaggca ccgacttcgc ctcctgccgc
     6361 aacgccctga attagacgcg ctagcaccag cgtcgcggtt ccccaatagc ccgccgtttc
     6421 atacgtcggt gcgatgccga tcatcagtgt accaagactc atcagcgcaa tggtgaacac
     6481 catcgcgggt ttgcgtccga aacgatcggc ccaggccccc acaataatgc cgccgagcgg
     6541 gcgcataaaa aagctcacgc cgaaagtggc aaacgccagc agtagcgcga cgtttttatc
     6601 tgcactgggc ggaaaaaata gcgtgctgat atagaccaca aaaaagttgt aaacgccaaa
     6661 atcaaaaaat tcgagcgcat tgccaaacgc cgaggcaaaa atcacccgcc tgccgctggg
     6721 ctttttcttc acactcacgg tttcatttac gatcgccgtc attccatgtt ccttgccaaa
     6781 aagtgttgtt gtgtgttgtt ttatttattg cagccaggct tctaccagtg cgccccagta
     6841 gctgcttgcc gggaccagac ttgcatcgtt gaagtcgtaa cccgggttat gcaccatgca
     6901 ggaaccttcc ccgacatcgc cattaccgaa taacaggaaa ccgcctttgg gttgggcttc
     6961 cagcatgaaa gagaaatctt cactgcccat caggggcttg atgccaaact cggcccgatc
     7021 ttcaccaaac gttttacacg ccacctgcca ggcgaagcgc gccatctcct cgtcatttac
     7081 taatactggt gtaccgttaa catgcgtaac ctcagccgta gcgccaaagc tggcagcctg
     7141 ggcttgtgcc agagcaggaa tacgggtcag aagaagttgt cgggtatcgc ggctaagaga
     7201 gcggacgctg attttcatct cagcactgtc aggaatgacg ttagccgcct cacccgcaac
     7261 aatgctgccg acggtaatta ccgccgcttc cagcggatcg acattgcgtg acacaatgct
     7321 ttgtaatgcg gtggtgatat gtgcggcaac tagcaccgga tcgatggctt tgtgcgggat
     7381 cgcaccgtgt ccgccacaac cacgaactgt aatatggaac tgatccattg acgccatcag
     7441 tgcccctggc tggaaataaa acttacccac cggcagaccg ggcatgttat gcatgccgaa
     7501 aatgacatcg caggggaaat gatcaaaaag cccctcttta accatgatct caccgccatt
     7561 aatcatctct tccgcaggct ggaagataag acgtaacgtg ccgttaaaac ggcgggtttc
     7621 ggcaaaataa cgcgcggccc ccagcagtat ggtggtatgc ccgtcatgcc cgcaggcgtg
     7681 cattaaacca ggatgtttac tggcccaggg cttgccacta ttttcataaa tcggcagcgc
     7741 atccatatcc gcccgcaggc caatactttt ctctccgtcg cccactttta acgtggccac
     7801 tatccccgtc gtcgccagtc cttgctctac ttcatagccc cactgacgca gcttttccgc
     7861 aaccaaagca ctggttttaa attcttgtaa tcccagctcc gggttttcgt ggatctgatg
     7921 gcggatctca cgcagttctt cctcaaaccc tcgaatatag tgttcgatca tcggctattc
     7981 ctttattcac
//
LOCUS       DBFIFM010000201         7838 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.201, whole genome shotgun sequence.
ACCESSION   DBFIFM010000201 DBFIFM010000000
VERSION     DBFIFM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.201"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            220..717
                     /gene="ubiC"
                     /locus_tag="N7C74_003935"
     CDS             220..717
                     /gene="ubiC"
                     /locus_tag="N7C74_003935"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HGU2433715.1"
                     /translation="MSHPALTQLRALRYCKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            730..1602
                     /gene="ubiA"
                     /locus_tag="N7C74_003936"
     CDS             730..1602
                     /gene="ubiA"
                     /locus_tag="N7C74_003936"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HGU2433716.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1757..4180)
                     /gene="plsB"
                     /locus_tag="N7C74_003937"
     CDS             complement(1757..4180)
                     /gene="plsB"
                     /locus_tag="N7C74_003937"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HGU2433717.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPVHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4351..4719
                     /gene="dgkA"
                     /locus_tag="N7C74_003938"
     CDS             4351..4719
                     /gene="dgkA"
                     /locus_tag="N7C74_003938"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HGU2433718.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            4829..5437
                     /gene="lexA"
                     /locus_tag="N7C74_003939"
     CDS             4829..5437
                     /gene="lexA"
                     /locus_tag="N7C74_003939"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HGU2433719.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5510..6835
                     /gene="dinF"
                     /locus_tag="N7C74_003940"
     CDS             5510..6835
                     /gene="dinF"
                     /locus_tag="N7C74_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HGU2433720.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVRGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTSWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLASLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            6951..7160
                     /locus_tag="N7C74_003941"
     CDS             6951..7160
                     /locus_tag="N7C74_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HGU2433721.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7202..7717)
                     /gene="zur"
                     /locus_tag="N7C74_003942"
     CDS             complement(7202..7717)
                     /gene="zur"
                     /locus_tag="N7C74_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HGU2433722.1"
                     /translation="MEKTTTQELLAQADKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1741 a         1933 c         2183 g         1981 t
ORIGIN      
        1 aaggaaaaca tcaccgctct tggcatcatg ggcggtgatg cccctatggt tgccctcgcc
       61 agcacgggca tcggcaaagc gtaaggttca acagcgtttt accacttcat gcgattgttg
      121 cgtttttgtt gcgcattaga tcacttaatt tgctttacat ctcccgtaaa cacttttctg
      181 cgatacaatg cctttacgtt atgtaacgga gagttcggca tgtcacaccc cgcgttaacg
      241 caactgcgtg cgctgcgcta ttgtaaagag atccctgccc tggagccgca actgctcgac
      301 tggctgttgc tggaagattc catgacaaaa cgttttgaac agcagggaaa aacggtgagc
      361 gtgacgatga tccgcgaagg gtttgtcgag cagaatgaaa tccccgaaga actgccgctg
      421 ctgccgaaag agtctcgtta ctggttacgt gaaattttgt tatgtgccga tggtgaaccg
      481 tggcttgccg gtcgtaccgt cgttcctgtg tcaacgttaa gcgggccgga gctggcgtta
      541 caaaaattgg gtaaaacgcc gttaggacgc tatctgttca catcatcgac attaacccgg
      601 gactttattg agataggccg tgatgccgga ctgtgggggc gacgttcccg cttgcgatta
      661 agcggtaaac cgctgttgct aacagaactg tttttaccgg cgtcaccgtt gtactaagag
      721 gaaaaaaata tggagtggag tctgacgcag aataagctgc tggcgtttca tcgcttaatg
      781 cgtacggata agccaattgg cgcgttactg ctgctctggc caacattatg ggcgttgtgg
      841 gtggcgacac cgggcgttcc ccagctctgg atcctggcgg tgtttgtcgc gggtgtctgg
      901 ctgatgcgcg ctgccggatg tgtggtgaat gattatgctg accgcaagtt tgatggtcat
      961 gttaagcgca cggcgaaccg accacttccc agcggcgcgg taacagagaa agaggcgcgc
     1021 gcgctgtttg tagtgctggt actgatttcg tttttactgg tgctgacgct gaatacgatg
     1081 accattctgt tgtcgattgc cgcgctagcg ctggcgtggg tgtacccgtt tatgaagcgg
     1141 tatacccatc taccgcaagt ggtgctgggc gcggcgtttg gctggtcgat tccaatggct
     1201 tttgctgctg tgagtgagtc ggtgccattg agttgctggt taatgttcct cgccaatatt
     1261 ctctgggcgg tggcttacga cacgcagtat gcgatggttg accgtgatga tgatgtgaag
     1321 attggcatta aatccaccgc aatcctgttc ggccaatacg ataaattgat tattggtatt
     1381 ttgcagattg gcgtactggc gctgatggcg attatcggtg agttaaatgg cttaggctgg
     1441 ggatattact ggtcaattct ggtggctggc gcgctgtttg tttatcaaca aaaactgatt
     1501 gccaaccgcg agcgcgaagc ctgctttaaa gcatttatga ataataacta tgttggtctg
     1561 gtactgtttt tagggctggc aatgagttac tggcatttct gatgatgtaa aaaagccgga
     1621 tgatcatccg gctttcttct gggttgcctg atgcgctgcg cttctcaggc ctacacaaca
     1681 gatcgcaatt tattgaattt gcagattatg gaaggccgga taaggcgttt gcgccgcatc
     1741 cggcaattct ctctgattac ccttcgccct gcgtcgcact ctcaatcgtc aaacgcacgt
     1801 ctgatgtaat caactccgcc agcaactgat aaaccttcat cgtttctgcc ggttcggcat
     1861 cgccgctatc gctgatatac ccttcatcac gcagcgtaag caccagagaa ctgaacaccg
     1921 ccttgtcgaa gaactccggc gcgttgatgc cgtgcagcac ggagagacgt tgcgcgacgg
     1981 tgcggctctc tttctccagc gtaccgcggt tgatcgacgg gttggcgctc aacagccaga
     2041 aggtgatggc ataacgttgc agcgtttcgc gcgcgcctgc ggcgagcagc tgtagcgtgc
     2101 gagaatgcac cgggttgata tgcaactcat catcttgcag ggtaatcagc ccctgacgtt
     2161 gcatctcatt tgccagcgca tcaataacgt ccggcaactc gtcgcgatcc cagcgcagga
     2221 acagctccgc tttcagcatt gggtaaagca cattgacgtg ctccatcaat acgtcgcggg
     2281 agatgtggcg atgctgggtg acaattgccg ccatcagcga aggtagcacc aacatatgcg
     2341 caatgttgtt gcgatagtag gtcatcagca ccgcttgctc gcgcggcaga atgatgatgt
     2401 cgccgatagt gtctttctca acttcaaact tgttcatttg cagcgcgtga tcgataagct
     2461 cgctggcgct ggctgaagga acggtagagt ccgtggagta aggcacgttg cgcatcagat
     2521 ccaggtagca gttgagttgc tcggttaact gctcgcgggt gagtgagcgc tgacgtgatg
     2581 ccagtagcgc agtacagcac aggttcatgg cgtttgccgc gcctgcgttg ttaatgcgta
     2641 ccatcagatc ggcagcaata ttattgaccg tcggcgttaa ccatgccgga cgcactgctt
     2701 cgatgggatc gatagattca cgccagtctg gtacgtgctg gttaaggtag gtcatcaacg
     2761 gcattggttc accgaaattg acgtaaccct ggccgagatt acgcagcttg cttaaaccgc
     2821 gcagcatctg cggcaggctc tctttctctt tcgtcgcgcc gcgcagttct ttggcgtaag
     2881 tacccacttc catgacgtgc tcataaccga tatagatcgg aatcagcgta atcggacgcg
     2941 tgccgccacg cagcatcgcc tgaatggtca tcgacagcgt accggttttc ggatccagca
     3001 aacgccccgt acgggaacga ccgccttcta cgaagtactc gacggaataa ccacggctga
     3061 acagttcgcc gagatactca cggaaaacgg tggaatagag cttattgcct ttaaaagtac
     3121 ggcgaataaa gaacgcaccc agacggcgga aaatcggccc ggcaggccag aaattcaggt
     3181 tgatcccggc ggcgatatgc ggcggcacta gcccctggtg gtacagcacg taagaaagca
     3241 gcaggtagtc catgtgactg cggtggcaag gcacatatac cagctcatga ccgtcgtggg
     3301 ccagctggcg aacgcgctcg gcgttatgga cgttgatgcc ctggtaaagg cggttccagg
     3361 taaagcccag aatacggtca gtcaggcgga tcatctcgta agagaaattc gccgcaatct
     3421 cttccatcag cgcaatcgcg ttctgctgcg ctttttcatg ggagattttt ttgctgcgcg
     3481 cttcatcttc taccgctttg gcaatggcgc gggaggcgag cagcttatta aacagatcct
     3541 gacgagcagg cagacgtggt cctacggcag ccagacgttg acgggcaaag tgcatacgcg
     3601 ccacgcgcgc cagtttctgc gcgatagttt tatccgtgcc gtgttcatcc gccatacggc
     3661 gcagcgaaac tgacggcgag aaacgcacaa aactgtcgcg accgagccac agtacagcga
     3721 aaaatttctg tacgccgtta agcatacgca gcggcgggtt cacttcgcct ttttcacgcc
     3781 ccggcgcgcg accaaacatc accgacactg gcaccatctg cacatccaga tttgggttgc
     3841 tacggtgcaa atcgagatag tcgtggaaca gcttaataga ctcttctttc ggcgtgtaat
     3901 aggtgaatac acgcggcccg ccgtgaatga acacatagcg cggcagtagc gtgccgtcga
     3961 tttccagcgg ctctaacggg tcaggcaagt catgtgccag acactgggcg cgcaacgtca
     4021 gcaaatctgc tttcgagttg tacggtaaaa cgtacataat tggacgagag gtatccagcc
     4081 ccagttccgg ggcaggatct gccggaatag acttgctttt taccaggatg cttaatggta
     4141 aattcagtaa tttgtagtaa attcgtggcc agccggacat aaacgatgta aagcctctgg
     4201 ttaataatgc aaatgcgcgg caaggatagc agaaagtcat gggaaattct gtggtatccg
     4261 ctcatgtttc gcgcggcgtt acgcaaaccc gaatcatcgg atttaacggt acactgatat
     4321 tgacgctcat aatgtaaaaa ggttctttca atggccaata ataccactgg attcacccga
     4381 ataatcaaag ctgctggcta ttcctggaaa ggtttacgcg ctgcatggat caacgaagcg
     4441 gcattccgtc aggaaggcgt agcggtattg ttggcggtgg tcatcgcctg ctggctggat
     4501 gtggacgcga ttacccgcgt gctgcttatc agctccgtga tgctggtgat gattgtggaa
     4561 atcctcaata gcgccatcga agcagtggtt gaccgaattg gctctgaata ccatgagctt
     4621 tccggacgcg ctaaagatat gggatccgcc gcggtgctga ttgcgattat cgtcgccgtg
     4681 attacctggt gcatcctgtt atggtcgcat tttggataac ccttccagaa ttcgataaat
     4741 ctctggttta ttgtgcagtt tatggttcca aaatcgcctt ttgctgtata tactcacagc
     4801 ataactgtat atacacccag ggggcggaat gaaagcgtta acggccaggc aacaagaggt
     4861 gtttgatctc atccgtgatc acatcagcca gacaggtatg ccgccgacgc gtgcggaaat
     4921 cgcgcagcgt ttggggttcc gttccccaaa cgcggctgaa gaacatctga aggcgctggc
     4981 acgcaaaggc gtaattgaaa ttgtttccgg cgcatcacgc gggattcgtc tgttgcagga
     5041 agaggaagaa gggttgccac tggtaggtcg tgtggctgcc ggtgaaccgc ttctggcgca
     5101 acagcatatt gaaggtcatt atcaggtcga tccttccttg ttcaagccga atgctgattt
     5161 cctgctgcgc gtcagcggga tgtcgatgaa agatatcggc attatggatg gcgacttgct
     5221 ggcagtgcat aaaactcagg atgtacgtaa cggtcaggtc gttgtcgcac gtattgatga
     5281 cgaagttacc gttaagcgcc tgaaaaaaca gggcaataaa gtcgaactgt tgccagaaaa
     5341 tagcgagttt aaaccaattg tcgttgacct tcgtcagcag agcttcacca ttgaagggct
     5401 ggcggttggc gttattcgca acggcgactg gctgtaacat atctctgaga ccgcgatgcc
     5461 gcttggcgtc gcggtttgtt tttcatctct cttcatcagg cttgtctgca tggcattcct
     5521 cacttcatct gataaagcac tttggcatct cgccttaccc atgattttct ccaatatcac
     5581 tgttccgttg ctgggactgg tcgatacggc ggttattggt catcttgata gtccggttta
     5641 tttgggcggc gtggcggttg gtgcaacggc gaccagcttt ctctttatgc tgttgctgtt
     5701 tttacgcatg agcaccaccg ggctgactgc gcaggcttat ggtgccaaaa atcctcaggc
     5761 attagcccgt gcgctggtgc aacctttgct gttggcgttg ggggctgggg cgttaattgc
     5821 gctgctgcgt acgccgatta tcgatctggc gctgcatatt gttggcggta gtgaagcagt
     5881 cctggaacag gcgcggcgct ttcttgaaat ccgctggtta agcgcaccgg cgtcgctggc
     5941 gaatctggta ttacttggtt ggttactcgg cgtgcaatat gcccgtgcgc cagtaatttt
     6001 gttagtggtc ggcaatatcc tcaacattgt gctggatgtc tggctggtga tgggactgca
     6061 tatgaacgtg cggggcgcgg cgctggcgac ggttattgcg gaatatgcaa cattgctgat
     6121 tggtctgcta atggtgcgta aaatcctcaa actacgcgga atctccggcg aaatgctgaa
     6181 aacttcctgg cgaggaaact tccgtcgctt gctggcactt aaccgcgata tcatgctgcg
     6241 ctcgctgttg ttgcaactct gtttcggcgc gatcaccgta cttggcgcgc gactggggag
     6301 tgacattatc gctgttaacg cggttctgat gacgctactc acctttaccg cctatgcgct
     6361 ggacggtttt gcctacgcgg ttgaagcaca ttccggtcag gcatacggtg cgcgcgacgg
     6421 tagccagttg ctggatgtct ggcgggcagc atgccgccag tcggggatcg tagcgttact
     6481 gttttcggtg gtttatttgc tggcagggga acacatcatt gcgttactgg cgtcgttaac
     6541 ccagattcag cagctggccg accgctatct tatctggcag gtgattttgc cgttggtcgg
     6601 cgtctggtgt tatctgctgg acggcatgtt tataggcgca acgcgtgctg ctgaaatgcg
     6661 taacagtatg gcggtggccg ccgcaggttt tgcgctgacg ctccttacgc tgccgtggct
     6721 gggtaatcat ggtttgtggt tggcgttaac cgtctttctg gcgttgcgcg ggctttctct
     6781 ggcagctatc tggcggcgtc actggcgcaa cggtacctgg tttgccgcaa cgtgacggtt
     6841 aaaaattctg aataaataat cctaagccaa attgctgact acacttaatc tcacgttcag
     6901 aagaaaagtg aacgtactct cattcacaac ctaacgatga ggtcttgatt atgaataaag
     6961 atgaagccgg cggtaactgg aaacagttta aaggtaaagt gaaagagcaa tggggcaaac
     7021 tgaccgatga tgatatgacg atcattgaag gtaaacgtga tcaactggtc ggtaaaatcc
     7081 aggaacgtta tggttatcag aaagatcagg cagaaaaaga ggtcgtggat tgggaaaccc
     7141 gcaatgaata tcgctggtaa ttaatccctc ctgcccgacg tgtacaagga tgtacgccct
     7201 cttaacgcgg tttctttttc acctgcacag agtgatcatg ctggcactgt tcaggatgac
     7261 gacacgcttc cacttccacg catgccgcac agagcccatg tgcttcaatc acattatgcc
     7321 gcagggcaaa ccccattttt gccgccagcg tatgcatgat gtcttccacg ccttcagcac
     7381 attcttcttt caccgcgccg cagcgatcgc aaataaacat agctgacgta tgggtgggct
     7441 ggtcgaacag atggcagagc acataactgt tggtggattc caccttatgc acaaaacctt
     7501 gctcaagcag aaaatccagc gcgcgataaa ccgttggcgg cttggcttgc ggttcagctt
     7561 cgcgcagcaa atcaagcaga tcataagcgc tgatagcgcc atcctgcaga ctcatcaggc
     7621 gcaacacttc caggcgctgt ggggtcaggc gcacattacg ctgcgcgcag attttgtcag
     7681 cctgcgctaa taactcctgc gttgtggtct tttccatcgg gcacctcaaa gtgggggtta
     7741 agaatgtcct tactttacca tgttccagga aaaacaccga gaacccttat tgttgccgta
     7801 atgttgcttt tctgttttgt aggtaagatg ttgtgttg
//
LOCUS       DBFIFM010000202         7753 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.202, whole genome shotgun sequence.
ACCESSION   DBFIFM010000202 DBFIFM010000000
VERSION     DBFIFM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.202"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..352)
                     /locus_tag="N7C74_003943"
     CDS             complement(<1..352)
                     /locus_tag="N7C74_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HGU2433723.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLAPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEF"
     gene            complement(609..1244)
                     /gene="msrQ"
                     /locus_tag="N7C74_003944"
     CDS             complement(609..1244)
                     /gene="msrQ"
                     /locus_tag="N7C74_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HGU2433724.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWIILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKSRSLFNRLRKQVHNKLSV"
     gene            complement(1245..2249)
                     /gene="msrP"
                     /locus_tag="N7C74_003945"
     CDS             complement(1245..2249)
                     /gene="msrP"
                     /locus_tag="N7C74_003945"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HGU2433725.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKALEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(2357..2770)
                     /gene="hiuH"
                     /locus_tag="N7C74_003946"
     CDS             complement(2357..2770)
                     /gene="hiuH"
                     /locus_tag="N7C74_003946"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HGU2433726.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2903..3574
                     /gene="hprR"
                     /locus_tag="N7C74_003947"
     CDS             2903..3574
                     /gene="hprR"
                     /locus_tag="N7C74_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HGU2433727.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3574..4932
                     /gene="hprS"
                     /locus_tag="N7C74_003948"
     CDS             3574..4932
                     /gene="hprS"
                     /locus_tag="N7C74_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HGU2433728.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNSYLNIDIASPGAK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(5040..5891)
                     /gene="hchA"
                     /locus_tag="N7C74_003949"
     CDS             complement(5040..5891)
                     /gene="hchA"
                     /locus_tag="N7C74_003949"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HGU2433729.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPQKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(6483..7669)
                     /gene="ompC"
                     /locus_tag="N7C74_003950"
                     /pseudo
     CDS             complement(6483..7669)
                     /gene="ompC"
                     /locus_tag="N7C74_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
BASE COUNT         2124 a         1655 c         1822 g         2152 t
ORIGIN      
        1 ggaattcaac aatgccgtcc tcaatgccaa tcatctcgat atctgttgca tagcctttgt
       61 gataataatc tttaatttca gcaaatgttt tagttttatc tgcatccgct ttcttctgaa
      121 agacgggggc aagtttgcca ctttgcagta aaggataaac ggattgccag actccatccc
      181 agtcactgag cgttcggttt tgtacattgg catcatcaaa aacaccatta gcagcttttt
      241 gttcgacctc tgttaagggt ttgccgtgtg agtgatgacc atgcgaaaag gcaggagcgc
      301 taacaataaa gacacctaaa gcaacagcca gtttgtgaag acgaatcgcc aaaacagttt
      361 cctccagagt cattgtatat gatgtgtaat gttatattgt aacatatgac aaaatgcaac
      421 aactcttagg agatgagagc atagttttct caggtgtact tatctatctt attgaaataa
      481 aatgaattat gttgataatt tgaggttgat gacaaacgat acataacctg atgtgatctg
      541 cgtaggccgg atagggcgct cgcgccacat ccggcaggga taacgcgtac tttgtcggca
      601 atatgaagtt atacagataa tttgttgtga acctgcttgc gtaaccggtt aaaaagggaa
      661 cgcgacttct tataccgtaa ggctaaaagc agtacagcca gcccagcgta gatgagcggc
      721 tgcggtgaaa taatcttcac tgaccacaga taatgtattg gggccaggat cgcgacaaga
      781 tagacgaaat tatgcaactg ttgccagtgc ttgccgagtt ttcgctgcat cgcctgggtt
      841 gaagtaaacg ctaaagcaag caaaataatc cagctgataa ttcctaacgt taaataaggc
      901 cgggtaatta actccttgcc taataacgcc agattattca cgcccagctc cagcaatgcg
      961 taactggtta aatgcagtgt cgcccaggca aagcaccata agcctaacag gcggcgtgtg
     1021 cgtatcaata acggctgttt tgcgtagcgt gccagaggtg tgattaacaa ggtcgccagc
     1081 aaaaatttca gtgcagtgcg accggtaaaa tgctgaatat ctttcaccgg atcggcaccc
     1141 agtccaccgt gattgatcgc ccagaccagc cagagaaacg gcaacaatcc ggcaagatgc
     1201 aggcaaactt tcagccatgt cacctgtttt gctgtcagac gcattcagaa attctcccgc
     1261 aaatccaggc cacgatacag cgatgccacc tggtcggcgt aaccattaaa cagtagcgtt
     1321 ggctggcgct gtacatcgag gatgccgcct gaaccaataa atcgttcggt agcctgtgac
     1381 cagcgtgggt gatcaacatg cggattaacg ttggcgtaaa aaccgtattc gtcaggcgct
     1441 gccagattcc aggtggttgg cggacgctcg cgggtcagct taatactgac gatcgattta
     1501 atccctttaa agccatattt ccacggcaca atcagtcgca ccggcgcgcc attttgtggc
     1561 ggtaacgcct tgccataaac acctacggtc atcagtgtga gcggatgcat tgcttcgtcg
     1621 agacgcaatc cttcgacata aggatatttc agcccgccgc cgataaagcg gtcctgctgg
     1681 cctggcatct gttccggtgc ataaattgtt tcgaaagcga catacttcgc attgctggtg
     1741 ggttcggcaa gcgccagcaa tttgtgcagc ggaaaaccaa tccacggcac caccatcgac
     1801 cacgcttcca cgcagcgcat acgataaata cgctcttcca gcgggaagcg acgggttaaa
     1861 tcatcgtgat cgagggtcaa tggttttgcc acttcgccgc tgattttcag tgtccatgga
     1921 tcggttttca ggctaccagc attagcggcg ggatcggctt tatccagccc gaattcatag
     1981 aagttgttat aaccggagac tttatctgct ggcgtcagtg gcaggttatt ttgccaggcg
     2041 gcaggcttgc tgaactccag cgcttttccg gcgggggcgg gtgggcgatc gttcccttta
     2101 aaccagctaa gcagatcggc atgcgcagcg tgaggcaaag aaagtgcagc tgcgctgatg
     2161 cccagtgctt ttaacacctg ccgacgcttc ataaagaata ccgactcggc cgtaacatct
     2221 gattctttta aaaattgatt ctttttcata agccatcaca ctttttgtcg ttagccagca
     2281 agagccgttt actgttaatc tgtcagggag tgtcctccct aacagtagca gagatctgct
     2341 tttacatatt tatgcattaa ctgccacgat aggttgaata cccatattgg ctaagtaata
     2401 aaggcacatg ataatgctcg ttcactttat taatatgaaa ttcaacgggg atctcaggga
     2461 agaaactttc aagattttgt ttcttgaaat agtccccggt tttaaatacg acacggtaat
     2521 cgcccgtagt tgcagtttgc tcgggccaca gtgccttaat tcgtccatcc ttatctgttt
     2581 tggcggtatt aagttgtaac cagccgttgt ccgctttctt ttcaagagtg actgtcacgt
     2641 cggcagcggg ttttcctgtt tgctggttca aaatgtgcac gctaagaatg ttttgttgtg
     2701 cagcatagac caaagaaggt aatgaaaatg cagccgttac tacggagagt actaaataac
     2761 gctttaacat gtttatatcc ttgtcatgtg aatgagtttt gaggatagcg aaaaagcgtc
     2821 cagggtaacc ttacagcagc attacaattt tgtaatgaac caggctgttt ctatgacata
     2881 tgatttatgg catattattt tcatgaagat tttacttatt gaagataatc aaaggaccca
     2941 ggagtgggta acgcaggggc tttccgaagc gggttatgtc attgatgccg tttctgatgg
     3001 cagagatggg ctttatcttg cgctgaagga tgattatgca ttgatcattc tggatattat
     3061 gcttccgggt atggatggct ggcagatctt acaaacgtta agaacggcaa agcaaacccc
     3121 tgttatttgc cttactgcaa gggattctgt cgatgacaga gtcagagggc tggacagtgg
     3181 agcaaatgat tatctggtaa aacctttttc attttctgag ttgctggcaa gggttcgggc
     3241 acaattaagg caacatcacg ctttgaattc aacattagaa atcagcggct taagaatgga
     3301 ctctgttagt caaagtgtga gcagggacaa tatcagtatt acactgacgc gcaaggagtt
     3361 tcagttactt tggctactgg cctccagagc tggcgaaatt atacccagaa cggttattgc
     3421 gagtgaaatt tggggaatca actttgatag tgataccaat acggtggatg tcgccattcg
     3481 caggctccgc gcaaaagttg atgatccttt tcctgaaaag ctaattgcca caatccgtgg
     3541 gatgggatat tcgttcgtag cggtaaaaaa ataatgaaaa gattatctat aaccgtccgt
     3601 ttaaccttgc tttttatatt gctgctgtct gttgctggcg ccggaattgt ctggactctc
     3661 tataatggcc tcgcaagtga gttgaaatgg cgcgatgata caacactcat taaccggaca
     3721 gcgcagatca agcagttatt aattgatggg gtaaatccag atacgttacc tgtatacttt
     3781 aaccggatga tggatgttag tcaggatatc ttgatcattc atggtgatgg catcaataaa
     3841 attgttaacc ggacaaatgt cagtgatgac atgttaaata acatacctgc tagtgagaca
     3901 atcagcgcag ctggcattta cagaagcatt attaatgata cagagataga tgctttacga
     3961 attaatattg atgaagtttc gccatcatta acggttactg tggctaaatt ggcttcagcc
     4021 agacataaca tgcttgaaca gtataaaatc aatagcatta taatttgcat tgtcgccatt
     4081 atactgtgct cagtattaag tccgctgtta atccgaacgg ggttacgaga gatcaaaaag
     4141 ttgagtggtg taacggaagc gctgaattat aacgatagcc gggagcctgt tgaggttagc
     4201 gcattaccga gagaactaaa acctcttggg caggcgttga ataaaatgca tcatgcttta
     4261 gtcaaagatt ttgagcgtct aagtcagttt gctgacgatc tcgctcatga acttagaacg
     4321 ccaattaatg cattactggg tcagaatcag gttacgctca gtcaaaccag aagtatcgct
     4381 gaatatcaaa aaacaattgc cggtaacatt gaagagctgg aaaatatttc gcggttaaca
     4441 gagaacatac tgtttcttgc cagggcagat aaaaacaatg ttttggtgaa actggactcg
     4501 ctttctctca ataaggaagt cgaaaatttg ttggactatc ttgaatatct ttcagacgag
     4561 aaagagattt gctttaaggt cgagtgcaat cagcaaatct ttgcggataa aattttactg
     4621 caacgaatgt tatcgaatct tattgttaac gctattcgat attcgccaga aaaatcgcgt
     4681 attcatataa ccagttttct tgataccaac agctatctta atattgatat cgccagccct
     4741 ggagcgaaaa ttaatgagcc tgaaaaactc ttccgtagat tttggcgggg agataattcg
     4801 cgtcattccg taggtcaggg acttggtctt tctttagtca aagcgattgc cgaattgcat
     4861 gggggaagtg ctacgtatca ctatctcaat aagcataatg tgttccggat tacgttgccg
     4921 caaagaaatt aacatagggc ttcagtacgc cataaattaa ccgtgaaaaa acgcagccac
     4981 gtcgtgagta ctaacgcggc tgcgattgat tatgcgctta cattcaaacg taacagggat
     5041 taacccgcgt aagctgccag catttcctgc gccgccagtt tacccaacgc attcgctgca
     5101 aaaggactgt cgccggtgag aagtttacgg tccttatgta ctcgcccggt gatgtcgtca
     5161 ttaatgatgt tcatgcccat tttcttcagt tcttcgccga agtaccaggt gagatgaccc
     5221 ggcatatagc caatctctgg cgtttgtttg tctgcggcgt ctgggaatgc gcaaatggaa
     5281 taaccattca gtggattatc gccgtggcga agcgccagaa aagccgccgg gccgtggcaa
     5341 agggagataa caaaacggtc atttttgatt gcccactgta aagcggcagc tacgtcctgg
     5401 ctttcaggta aaccaataag tgcgccatga ccaccaggaa caaagattgc tgcatattcg
     5461 ctatcagcgt tgaggctggc gacaacatcc gcgagtttct tcggattgcg gaacaacgat
     5521 ttatgctgct caaagaatgg cattactttt tcatctttct gcggcatagc ccagtattca
     5581 aacttggtca tcagaccgga aatggtcgcc acttcgaatt cgaaacctgc agcatggaga
     5641 tgatacaacg gcagcaacgt ttcaatcgga tggttaccgg tcgagaacag ttttccgtta
     5701 tcggtcggca aataacgttc gtccgcggcg atcaccagaa ttttatgttt accgcgataa
     5761 ggttttggat agtccacgcc atcaagatca gagacaggac tggtatattg gctaagcgaa
     5821 tattctgaag ggaagaatgc attatcttca gcaatatcga cctgcggatt tttactggtt
     5881 tgaacagtca tagtgtattc cttattgttg cttagttagg gtagtcacta tatttgcgac
     5941 tgagctggtc gctattttta accaggctgg aaaattgagt caatatatcg aaagcggtag
     6001 tcggcgggtg taagtatgta atgatgatgc tgcactggtg ttgaatatta ttcacgccaa
     6061 attttcctta ttgagcaact tcgtcgcaag tggcctcaat gattttagac cattatcaga
     6121 ggtacggggc ggcggggaga gatttagtgc tgtggaatat tgtggtgctg atgaatattt
     6181 atatcgcgga ttggtagaga aaaagcttat ttcatgcact atttttgcaa atatttctat
     6241 atgggaagcg tgtttgttac ggattaattc ataaagaaat gcatttgggt gttggcttga
     6301 agagtcgctg gttgatgtta ttgtgccagg aggggcaggg taagggggtg tatcgataag
     6361 ctttggcaat tgcgtgtgcc gcagatggag atgaaatgcc aataaaaaag cctgagttcg
     6421 atgagggacg aactcaggct agtgttcagt tttacaataa cacgccgcgg cgcgttggct
     6481 gattagaact ggtagactaa gcccacagcc acgatatcat cggtagcgat gccgttttct
     6541 ttgtagaagt cgtcgtcgtt gtccagcagg ttgattttgt aatcaacata agtggacatg
     6601 tttttgttga agtagtaggt cataccaata tcaacatatt taaccagatc tttgttggtg
     6661 taatgatgat taccctggct gtcgaagtta tcactattta caccgcctaa atccttacct
     6721 ttagattgca gataagcgat ggacgggcgc agaccgaagt cgaactgata ctgagcaaca
     6781 acttcaaagt tttgggtttt gttagcgatg gcatcagcat caccgtaacc agtcatgtta
     6841 cggggttcag catacatagc tgctaagtaa acgttattag cgtcgtattt agcaccaacg
     6901 gtccatgctt ctgctgtttc accgccagca gttgcatcga agcgatctcc ataacgagtg
     6961 ttatgagtac ctttgtgaac ctgattgtcg gtacggtcag agttagagta agcagcgccc
     7021 agacttaaac caaagtcaaa gtcataggaa gtggacatac tgaaccgtcg ccgttctctt
     7081 tagcaagttt acgtccatta ccgttacctg aaccttcttg atcaaataat ccgcttttct
     7141 cgttgttacc ctgatactgg agtgcaaagt tcagaccatc aacttgacca aagaaaccat
     7201 tgttacgata ggttgctacg ctgttagcac gaccgttcat gaagttgtca gcgccagcat
     7261 aggtatctcc accaaattct ggcagcatat cggtccatgc ttctacgtca tatacgacac
     7321 cgtagttacg accatagtcg aaagtaccat tctgaccaaa gccgaaacct gcaaatgcca
     7381 gacgcgttgc ggtattaccc ttgtcgcttt caggaccgtt tgcgccaatg ttaaattccc
     7441 actgaccata accagtgaat tggtcagcga tctgagtttc acctttgaaa ccgatacggg
     7501 catatgacat gtcgccatcg ctgctagcat catcagagaa gtagtgcagg cccgctactt
     7561 ttccgtacaa atccagttta ttgccatctt tattataaat ttcagccgca tttgctgcgc
     7621 cagcaactaa taacgccggg accagcattg ccagaacttt tcttttcatt atgtattccc
     7681 ttgtgattat aatcttcatg aatatatcaa taagtgccgt tatccaaaaa aaagcacatt
     7741 tggatactat tct
//
LOCUS       DBFIFM010000203         7712 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.203, whole genome shotgun sequence.
ACCESSION   DBFIFM010000203 DBFIFM010000000
VERSION     DBFIFM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.203"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(85..348)
                     /gene="cedA"
                     /locus_tag="N7C74_003951"
     CDS             complement(85..348)
                     /gene="cedA"
                     /locus_tag="N7C74_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HGU2433730.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            531..2792
                     /gene="katE"
                     /locus_tag="N7C74_003952"
     CDS             531..2792
                     /gene="katE"
                     /locus_tag="N7C74_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HGU2433731.1"
                     /translation="MSQQNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTASGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2839..3597)
                     /gene="chbG"
                     /locus_tag="N7C74_003953"
     CDS             complement(2839..3597)
                     /gene="chbG"
                     /locus_tag="N7C74_003953"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HGU2433732.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMNFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSHGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3610..4962)
                     /gene="celF"
                     /locus_tag="N7C74_003954"
                     /gene_synonym="chbF"
     CDS             complement(3610..4962)
                     /gene="celF"
                     /locus_tag="N7C74_003954"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGU2433733.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNGVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5067..5909)
                     /gene="chbR"
                     /locus_tag="N7C74_003955"
     CDS             complement(5067..5909)
                     /gene="chbR"
                     /locus_tag="N7C74_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HGU2433734.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(5917..6267)
                     /gene="chbA"
                     /locus_tag="N7C74_003956"
     CDS             complement(5917..6267)
                     /gene="chbA"
                     /locus_tag="N7C74_003956"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HGU2433735.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6318..7676)
                     /gene="chbC"
                     /locus_tag="N7C74_003957"
     CDS             complement(6318..7676)
                     /gene="chbC"
                     /locus_tag="N7C74_003957"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HGU2433736.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVVALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
BASE COUNT         2074 a         2019 c         1721 g         1898 t
ORIGIN      
        1 tacaggtggt ttgaatgaat gtagcaatga aatagagaat ttcaggccat gtacctcccg
       61 gcaaaaccgg gaggtacgta atctttactc agtcacttcc ccttcctggc ggatctgatt
      121 tgcccaacgc tgggcggatt caggcacagt aaacgccggt gagcgcagaa atgactctcc
      181 catcagtaca aacgcaacat atttgccacg cagcatccag acatcacgaa acgaatccat
      241 ctttatcgca tgttctggcg gcgcgggttc cgtgcgtggg acatagctaa taatctggcg
      301 gttttgctgg cggagcggtt tcttcattac tggcttcact aaacgcatat taaaaatcag
      361 aaaaactgta gtttagccga tttagcccct gtacgtcccg ctttgcgtgt atttcataac
      421 accgtttcca gaatagtctc cgaagcgggg tctggctggt ggtctatagt tagagagttt
      481 tttgaccaaa acagcggccc tttcagtaat aaattaagga gacgagttca atgtcgcaac
      541 aaaacgaaaa gaacccacat cagcaccagt caccactaca cgattccagc gaagcgaaac
      601 cggggatgga ctcactggca cctgaggacg gctctcatcg tccagcggct gaaccaacac
      661 cgccaggtgc acaacctacc gcctcaggga gcctgaaagc ccctgatacg cgtaacgaaa
      721 aacttaattc tctggaagac gtacgcaaag gcagtgaaaa ttatgcgctg accactaatc
      781 agggcgtgcg catcgccgac gatcaaaact cactgcgtgc cggtagccgt ggtccaacgc
      841 tgctggaaga ttttattctg cgcgagaaaa tcacccactt tgaccatgag cgcatcccgg
      901 aacgtattgt tcatgcacgt ggatcagccg ctcacggtta tttccagcca tataaaagct
      961 taagcgatat caccaaagcg gatttcctct cagatccgaa caaaatcacc ccagtatttg
     1021 ttaggttctc taccgttcag ggtggtgctg gctctgccga taccgtgcgt gatatccgtg
     1081 gctttgccac caagttctat actgaagagg gtatttttga cctcgttggc aataacacgc
     1141 caatcttctt tatccaggat gcgcataaat tccccgattt tgttcatgcg gtaaaaccag
     1201 aaccacactg ggcaattcca caagggcaaa gcgcccacga tactttctgg gattatgttt
     1261 ctctgcaacc tgaaactctg cacaacgtga tgtgggcgat gtcggatcgc ggcatccccc
     1321 gcagttaccg caccatggaa ggctttggta ttcatacctt ccgcctgatt aatgccgaag
     1381 ggaaggcaac gtttgtacgt ttccactgga aaccactggc aggtaaagcc tcactcgttt
     1441 gggatgaagc acaaaaacta accggacgtg acccggactt ccaccgccgc gagttgtggg
     1501 aagccattga agcaggcgat tttccggaat acgaactggg cttccagttg attcctgaag
     1561 aagatgaatt caagttcgac ttcgatcttc tcgatccaac taaacttatc ccggaagaac
     1621 tggtgcccgt tcagcgtgtc ggcaaaatgg tgctcaatcg caatccggat aacttctttg
     1681 ccgaaaacga acaagcagca ttccatccag gtcatattgt tcctggtctg gacttcacca
     1741 acgatcccct gttgcaggga cgtttgttct cctataccga tacacaaatc agtcgtcttg
     1801 gtgggccgaa tttccatgag attccgatta accgcccaac ctgcccttac cataatttcc
     1861 agcgtgacgg catgcatcgt atggggatcg acactaaccc ggcgaattac gaaccgaact
     1921 cgatcaacga taactggccg cgcgaaacac cgccgggacc gaaacgcggc ggttttgaat
     1981 catatcagga acgcgtggaa ggaaataaag ttcgcgagcg cagtccatct tttggcgaat
     2041 attattccca tccgcgtctg ttctggctaa gtcagacgcc attcgagcag cgccatattg
     2101 tcgatggttt cagttttgag ttaagcaaag tcgttcgtcc gtatattcgt gagcgcgttg
     2161 ttgaccagct ggcgcatatt gatctcactc tggcccaggc ggtggcgaaa aatctcggta
     2221 tcgaactgac tgacgaccag ctgaatatca ccccacctcc ggacgtcaac ggtctgaaaa
     2281 aggatccatc cttaagtttg tacgccattc ctgacggtga tgtgaaaggt cgcgtggtag
     2341 cgattttact taatgatgaa gtgagatcgg cagaccttct ggccattctc aaggcgctga
     2401 aggccaaagg cgttcatgcc aaactgctct actcccgaat gggtgaagtg actgcggatg
     2461 acggtacggt gttgcctata gccgctacct ttgccggtgc gccttcgctg acggtcgatg
     2521 cggtcattgt cccttgcggc aatatcgcgg atatcgctga caacggcgat gccaactact
     2581 acctgatgga agcctacaaa caccttaaac cgattgcgct ggcgggagac gcgcgcaagt
     2641 ttaaagcaac aatcaaggtc gctgaccagg gtgaagaagg gattgtggaa gctgacagcg
     2701 ccgacggtag ttttatggat gaactgttaa cgctgatggc agcacaccgc gtgtggtcac
     2761 gcattcctaa gattgacaaa attccggcgt aaatatggat aaggtcacgt cccctgggga
     2821 gtgaccttac caccttactt acacatcaag ataactaccc aaccgataac cacgctcagc
     2881 aattgcatat ttcaacgacg ctgatgtcag cacttccagc tccgttaacc gtgggaaaca
     2941 gtaagcactc tggcgaatcg tattatcgac aaacgccgga tggcacatga cttccagcga
     3001 gcgttccccg cgatggctgg aatcatcaag cacttgcagg aacagtgcct cgctaatctc
     3061 ttcaccataa aatgcgctac tgaatccgtg cgaactgcgc agattagccg gtaaatcacc
     3121 agcgttagat agcggctgac ggtcgatgcg caacgcaatc ccctcttcag ccgcaaacct
     3181 ggcaacaata gggaaaatct gcgggaacat atgcacatga tgatggctat caagatgcgt
     3241 aggtttgcgt ccaaatagct caatgaaacg caaatactga ctggcaagct cctgagtaat
     3301 ttcttccagc ggtaaagcat cttcttctgc caactgccag atccattttc ccagcacacc
     3361 atcgcgggtt aaccccggca tagctgtcag tggcttgccc atagtaagga caaagttcat
     3421 ccctatggcc agactcggtt catcacgact caactgcacc gcatggtcaa tagcctgccc
     3481 attcaccagc gccgtcgtcg atgtgacaat cccattgcga caggcctcga taatgccgta
     3541 gttctgacct ttgcttaagc caaaatcatc ggcattaaca atcagtaagc gttccataat
     3601 cagcctcggt taatgtgctt ttttaagctc tgcgatgcag tcggcaaaat ttggcagcca
     3661 tttctcgtgc gccagaatca tctcgcgtgc cagcagctca gcatcgcgat cggaatgcac
     3721 caacggacta aggtttagcg ccagtaacac accgttaaat tctccgctaa gtgcggcgtt
     3781 gctggcagca atctcgaagc ctttaatggt gtgaatcagc cccatcactt tatcatcgaa
     3841 atgcgtaatg cgcggatgtg gcgtcgcgcc atcgcgcccc agcgtacagg tcatttctac
     3901 cgcccagtct gccggaatat tatcaatatg cccatgatgc gggatattaa cgtaatgttc
     3961 tgcttgctta tcgttgtaga tagcgttgat cacttcgcac gctgcatcag agtaataagc
     4021 cccaccgcgc tgttccagtt ctttcggctt aactttcaac tccgggtttt tatacagctc
     4081 aaaaagttgt ttctcgactt tctgtactac ctgcgctcgt gcgccgcctt tgtagtattc
     4141 gcccatttca atagccagca tctctttttg cttgaagtaa taaagcaaat aagaacacgg
     4201 caacagattc agagaacgaa ttaagccttc gctaaatggc agatcgaaaa tatttttaac
     4261 gccagatgct tttaactgcc cggatgccac accatcaagc aattcggcaa agcgcgactt
     4321 gccatttacc agcacatcct taatgaacac catatggttg aggccgaaca gatcgataga
     4381 taaatcatcg ctgtctttca gcatcagaac atcgcgaata aacatcttca tgccgatcgg
     4441 aatattacac acgccgataa agcgtttaaa tccggtatga cgataaacgg cttcagtgac
     4501 cattcccgcc gggttagtga agttaatcac ccatgcattc ggacaaagtt cttcgacatc
     4561 ttttacgatg tcaaaaatca ccggaatggt acgcagacct ttaaacagac cgcccgcgcc
     4621 gttggtttcc tgaccaagat aaccatgact taatggaata cgttcatcca gttcacgcgc
     4681 cggtaattgg ccaacgcgca gttgggtagt aacgaaatca gcatctttca atgcttcgcg
     4741 gcgatccagc gttttataaa gcttcatcgg gacgccagcg ttatcaatca tccgttggca
     4801 gagatcgaaa ataatatcca gtttagcttt gccaccttcg acatccacca gccataattc
     4861 gctgaccggc aattcgtgat aacgcttaat aaatccttcc agtaactccg gggtatagct
     4921 gctcccgcca ccaatagtga cgacttttaa tttctggctc ataatttctc ccttcagtac
     4981 agaatactga tatctggcat atctgccccc ccggacataa ataatccagc aacaggacag
     5041 atatgtgaat tgtcaggtat aacgacttac tgattaaatt cagtcaattt cttacgatag
     5101 ctcttaggcg taaaggacgt taatttctta aacgttttaa taaacaaact cgggctacta
     5161 taaccggcct caaacgcaat atccgtcact gaatagttgg tcatttccag ttgttttttg
     5221 gcaaaattaa tacggatttc attaataatc tgcattggcg ttttgccata atatcgttga
     5281 gtcgcccgcg tcaaatattc ctgtgatttg gctgacaacg tcaccatatt ctccagcgcc
     5341 gattcactaa actgctcttt atcatgcatc ttttctaccg tacttttcag ccactgcggt
     5401 acatcatcaa tcacctgttc ttcgcggtaa tgacgtaaac ggttaatgac ataaaaagta
     5461 accatctcaa caaactcttc cagccctgtt tcgcggaaat tcaatgaaga aatcactgtt
     5521 tccacatagg tgagaaaagc gttattggtc cggtatacct gcgaagctac aaagcaataa
     5581 ggcaacaatg gcaggtaatg ctgctcaaaa aagcgtttac tgatcccaac gttcaatatg
     5641 cgcgtggcac caaactcata aaaactttga tggtgcgaac ctaacggaat aaaaacaaaa
     5701 tcgccccgtt ccagtaacac gcgcttaccg ttaatctcct ggaaataacg cccggttaat
     5761 accagagtaa attcataata gtcgtgctga tgcagtccgc tgatactctc agttttgtta
     5821 tagataaaca catggaagtt tttgccatta aacaactgct gttctcgggc agtggcaatt
     5881 tccggcgcgt taatcactgg ctgcatcatc gactccttat gccttcagtt tttcatgaag
     5941 ctcaattaat tcagtaatca gttcacgcgc aagcatggac gtcattaaat gatcctgagc
     6001 gtggaccagc accagactca ctttcatctt accttcgccc gcatcgcctt caatcagttt
     6061 cgtctgtacc agatgcgctt cattcaatgc catccgtgac tgatccatca tggcttttgc
     6121 tgcggcaaaa tcgccctgct tcgcctgttt cagtgcggca tacgccaggc tgcgcgcttg
     6181 tccggagttg atgataagcc ccatcaccac ttcttccagc tcttcagctt ccgtttgcgt
     6241 atcgggaata ttatcgagat ccatcataca tcgttcctct cttttcttac cggcacgatt
     6301 acccgtaccg gcatcgatta aaatttcagg gcgttagcga tatcttcttc gctctcttct
     6361 ttatcaatcg cattttgtgc tttgttagcc accacaacaa agggcagata aattaacgtt
     6421 gcgataccaa ggttgaagag tgcaaccagc aatgcgacga cgctaccgtt ggtgttaaag
     6481 aaggctccca gaccggttgg catggtccac ggtgcaatat tggtcaccgg aggaataatg
     6541 cccatgtagt acgctgccag ggtgattgcc gccagaatcg gttgtaccag tacaaacggg
     6601 ataaacatca ccgggttcat gataattggc agaccaaaca gaatcggttc gttaatctgg
     6661 aagatgccgg acggcagcgc cagttttgcc acctgacgat aatcagcacg gcgagaggca
     6721 ataaagatag ccaggatcag gcctaaagtc gcaccactgc ctccaaggaa aataaaggag
     6781 tccagcatcg gcttggccca gatatggaag gtcttaccgg ctgccagcgc cgcttcgacg
     6841 gaaccatatt gctgataggt cgcgatattt tccagcgccc acggcgtcat aatgccgttg
     6901 tccagtgcgg tcagcgccag cgcgccatga ataccgaaga accagagcag tggaacaaag
     6961 atcacatagg cccagcccac cacgctaccc aacgatgcca gtggggttga gatggtatcc
     7021 ataatgatct gatggaagtt ggtgccccag gtattcaacg cccaggcaat aatccccatc
     7081 acggaaagaa taataaagcc gggaattaat gccgagaagg aacgcgatac tgaagcaggt
     7141 acgctgtcgg gcagtttaat gacccaattg cggcggacaa taaaggtaaa catttctgcc
     7201 accaccaggc caataataat cccggagatg atattcgccc cacctaacca gtttgcgcca
     7261 accgcatagg cctcaccgac actatatggg gtgacggtca taaatgctgc aacggataac
     7321 aacccagccg ccagcgcatc gactttacgc tcttctgcca gcgccatgcc aataaagaac
     7381 ggtgccatta aagacattat ccctaatgtt ccgttatata cgttgccgcc aatacctttc
     7441 agaccattaa gtgtttcaat ggttgaggcg tcgaggcgaa tacctaagga ataaaaaaac
     7501 gacccctccc caaagcttag aaaaacgttg ttaattaata caaacatggc ccccgcaagg
     7561 gttaacggca ttaagcgaat aaagccattt ttgattgcat taacgtgtgg ctgctttcct
     7621 attttaactg caaaagggag gagtaccttt tcaagcgatg caataacatt actcatagaa
     7681 aaataccctt aaaaaccgca atttaaatat tg
//
LOCUS       DBFIFM010000204         7709 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.204, whole genome shotgun sequence.
ACCESSION   DBFIFM010000204 DBFIFM010000000
VERSION     DBFIFM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.204"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(12..218)
                     /locus_tag="N7C74_003958"
     CDS             complement(12..218)
                     /locus_tag="N7C74_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2433737.1"
                     /translation="MNQHNTRLIRLPEVLHRTGYGKAWIYHLINQNRFPSPVKIGARS
                     VAFIESEVDEWIQLTIENSRKHVA"
     gene            complement(318..1169)
                     /locus_tag="N7C74_003959"
     CDS             complement(318..1169)
                     /locus_tag="N7C74_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6387 family protein"
                     /protein_id="HGU2433738.1"
                     /translation="MKNWSVEHTREVKKWLDIDTYRGFEELPLIHLYHELLARTLFFK
                     PYHEEFEAEAVRLYIDRIFTGKPFLITEKHLGYLTREDTLYQPPHFFLTTTERLAQLS
                     IVGLRNSLFFWDGSDEYSVNREFLDSPVSEVLPKLFRDTVMVEIDLANGTDEEIAESL
                     KAALPQWRKVRGIEPDVTEAIRFGYGTIKKIINYRLLPMIDILVWSKLYKVRISEDRL
                     SRLLYTDDDDEINTRLNHQIRDTDKPLALKAASIPFIRQFNLFINKNSHLKKIRVSDV
                     MKIADSD"
     gene            complement(1596..6182)
                     /gene="ypjA"
                     /locus_tag="N7C74_003960"
     CDS             complement(1596..6182)
                     /gene="ypjA"
                     /locus_tag="N7C74_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HGU2433739.1"
                     /translation="MNRTSPYYCRRSVLSLLISALIYAPPGMAAFTPDVIGVVNDETV
                     DGSQRVDERGTTNNTHIINHGQQNVYGGVSNGSLIESGGYQDVGRHNNYVGQSNNTTI
                     NGGRQSIHDGGISTGTIIESGNQDVYKGGISNGTTIKGGASRVEGGSANGTLIDGGSQ
                     IVKVQGHADGTTINKSGSQDVVQGSLATNTTINGGRQYVEQSTVETTTIKNGGEQRVY
                     ESRALDTTIEGGTQSLNSKSTAKNTQIYSGGTQIIDNTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNYGIATGTNIN
                     SGTQNIKSGGKADTTIISSGSRQVVEKDGTAIGSNISAGGSLIVYTGGIAHGVNQETG
                     SALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVVAKTSAKNTTIDAGGK
                     LIVQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDA
                     FYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGTVLNSAGTQTIY
                     ASATSDKANIKGGKQTVYGLATEANIESGEQIVDGGSTEKTHINGGTQTVQNYGKAIN
                     TDIVSGLQQIMANGTAEGSIINGGSQIVNEGGLAENSVLNDGGTLDVREKGSATGIQQ
                     SSQGALVATTRATRVTGTRADGVAFSIEQGAANNILLANGGVLTVESDTSSDKTQVNT
                     GGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTTINEGGTL
                     TVNDNGKATDIVQNSGAALQTSTANGIEISGTHQYGTFSISGNLATNMLLENGGNLLV
                     LAGTEARDSTVGKGGAMQNQGQDSATKVNSGGQYTLGRSKDEFQALARAEDLQVAGGT
                     AIVYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAIRITDSATLTIGNGVDTTLAD
                     LTAASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGNVYLSAQTAAPATTNGIYNTLTT
                     NELSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGG
                     DASFSLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPNPNPNPNPKPDPKPDP
                     KPDPKPDPTPEPTPTPVPEKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASP
                     HNNNVWGATYNTRNNVTTDAGAGFEQTLTGMTVGIDSPNDIPEGIATLGAFMGYSHSH
                     IGFDRGGHGSVGSYSLGGYASWEHESGFYLDGVVKLNRFESNVAGKMSSGGAANGSYH
                     SNGLGGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELG
                     ATLSYNMRLGNGMEIEPWLKAAVRKEFVDDNRVKVNNDGNFVNDLSGRRGIYQAGIKA
                     SFSSTLSGHLGVGYSHGAGVESPWNAVAGVNWSF"
     gene            complement(6247..6366)
                     /locus_tag="N7C74_003961"
     CDS             complement(6247..6366)
                     /locus_tag="N7C74_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HGU2433740.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(6590..6748)
                     /locus_tag="N7C74_003962"
                     /pseudo
     CDS             complement(6590..6748)
                     /locus_tag="N7C74_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(7118..>7709)
                     /locus_tag="N7C74_003963"
     CDS             complement(7118..>7709)
                     /locus_tag="N7C74_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433741.1"
                     /translation="CYTFLPRAQPQDKIDSYNSALQAFSIFCSSTLTHNNVGFDFKLF
                     PEVKLSGEHLETVFKYKNGDDVREIAKINITLQKEEGGLYNLRGLDFKGCFFSGQNFS
                     NYDIQYVNWGMSLFDVDTPCIFNTPANHESYEKSLKPVSENGLNGVLSDRNKKIKMIT
                     GVAPFDDILFMDDDFDDNSPEDAPIENSPVVNSPLV"
BASE COUNT         1927 a         1906 c         1644 g         2232 t
ORIGIN      
        1 gtcattttat ttcaggcaac atgctttctt gaattttcga tagttaattg aatccattcg
       61 tctacttcac tttcaatgaa agcaacagag cgcgcaccga ttttaacggg ggaaggaaaa
      121 cgattttggt tgatgaggtg atagatccaa gctttaccgt atccagttcg atgcagaacc
      181 tctggtaaac gaataaggcg ggtgttgtgc tgattcatag tcgtttcctc tgtgccgttt
      241 tgatggaaat gactatacaa atgaatcagc aggaaaaaga ccgaagctgg gaacgccaga
      301 gctaatggag gttggtctta gtcagaatca gcgattttca tcacgtctga aactctgatc
      361 tttttcaaat ggctgttctt gttgatgaac agattaaact ggcgaataaa aggtatactc
      421 gccgccttca aagcaagagg tttgtctgta tccctgattt ggtggttcag gcgagtgttg
      481 atctcatcat cgtcatctgt atacaacagg cgagaaaggc ggtcttctga tatacgtact
      541 ttatataatt ttgaccatac cagaatgtcg atcatgggaa ggagtctgta attaatgatt
      601 ttcttaatcg ttccataacc aaagcgaata gcttccgtta catctggttc tatgccacgc
      661 actttacgcc attgaggaag tgcagccttt agggattcag cgatctcttc atctgtcccg
      721 tttgctaggt cgatttcgac catgacagta tccctgaaca attttggcaa aacttccgat
      781 acagggctat cgaggaattc tctgttaaca gaatactcat cgcttccatc ccaaaaaaac
      841 agtgagttcc gtaatccaac tatgctgagt tgagccagtc tttcggttgt cgtcaaaaag
      901 aagtgcgggg gctggtaaag agtatcctct cttgtgagat agccgagatg cttttctgtg
      961 atgagaaagg gctttccagt gaagatcctg tcgatgtaga gccttacggc ttctgcttca
     1021 aattcttcat gatatggttt gaagaacaat gtccttgcca gcaattcatg gtagaggtgt
     1081 ataagcggaa gctcttcgaa tccgcgatat gtatctatat ccagccactt cttcacctct
     1141 ctcgtatgtt caactgacca gtttttcata acgatctcca acaggcatta gacttaatgt
     1201 atgtagcaaa cgtaatcgac atgttaaagg aagaccaatg aaatgagttc agatgccatc
     1261 aggaagttga accaaaaggt ttgatatttt ttgatacagt tcgctaacgg actattttca
     1321 ggggtttatg ccgttacgat ttatggttcc tttatagaac caaaggtaaa gtcagaaaca
     1381 gcagttatct agaaaattca atcaaataga tatctagtta cagggacgcc agccaatcat
     1441 gattggacgg tgtaaggaca acaccaacaa aaacaggaag ttagaagtct cagcaggaca
     1501 ccgaccagac ggtgaggaga cataaaagga tacgcaaagg agccgcggct cctggtgaca
     1561 tgaaagccca cagatgtggg ctttttcgtt gatggtcaga acgaccagtt cacaccagct
     1621 accgcgttcc acggggattc cacaccggca ccatggctat accccacccc aagatgcccg
     1681 cttaacgtac tgctgaatga ggctttaata cctgcctggt atattccacg tctgcccgac
     1741 aaatcattga cgaaattacc gtcattattc accttcaccc ggttatcatc gacaaattct
     1801 ttgcgcacag ccgccttcag ccacggctca atttccatac cgttccccag acgcatgttg
     1861 taactcagcg ttgcgcccag ttcacgatat atactgcggg tatcgactga tttcgattcc
     1921 atgccattgg ataaatgata ttcggggtta tcagcggtga accccgttaa cgatgcatac
     1981 ggcgtcaggt tccagttacc atcggtaaat cgcatcccgg tttcaatgtg accgcccagc
     2041 ccgttgctgt ggtaactgcc attggcggct ccaccgctgc tcattttacc ggctacgtta
     2101 ctttcaaaac ggttcagctt cacgacaccg tccagataga aaccactttc atgttcccaa
     2161 ctggcatagc cgcccagaga ataactgccc acactgccat gtcctccgcg atcaaaaccg
     2221 atatgtgaat gggaataacc cataaaagcg cccagcgtcg caatcccctc aggaatatca
     2281 ttagggctgt cgatccccac tgtcattccg gtcagcgtct gctcaaaccc ggcccccgca
     2341 tcggtggtga cattattacg ggtgttatac gtcgcccccc agacattatt gttgtgtgga
     2401 ctcgctttca ttatgttcaa ccgctcgcga atactgttta gctcagcatc aaataccaac
     2461 ggtaatgttg ctgccatatt gagtacggct gcggtagaag gcgtgatgcg tttctccgga
     2521 acgggtgtcg gcgttggctc gggagtcggg tctggtttcg gatccggttt tgggtctggt
     2581 tttggatccg gcttcggatt tgggttggga tttgggttgg gatccgggtt gggtttgaca
     2641 tcattggtca ggttccagtt gctgttgcca tcgcttttca ggacatactc ataggtccca
     2701 agatcaacga aaccgccagt attgcccagc gaaaacgaag catcccctcc ccctgttttc
     2761 accagcgtca tcgcgtcgtc agactgagga ctgacgccgg tatcctgaac aaagatttta
     2821 aaattaccag tggcgttgtt gttgacgacc agttgatcgc cccgagagcc tgcaacgttg
     2881 gtatgcaggt agaaattacc gctaccggaa agttcattgg ttgtcagcgt attgtatata
     2941 ccgttagttg tggcaggcgc tgctgtttgt gctgataaat aaacattacc gtcgttaagt
     3001 agcaaactgt ttactctata ctcgcagttg ctggtgcctg cacaggaatt attgctgtta
     3061 agccagacac tgccccggct ggcagccgtc aggtcggcaa gcgtcgtatc aacgccattg
     3121 ccgatagtta atgtcgcgct atcggtaatc cggatcgccc cttcgagttt aactggcgta
     3181 acattatccc gtggcgtcat taacgacagg cttcctgtcg cgccactgac cgatgcatcc
     3241 gccagcgtac ctgcgtagac gattgctgtc ccgccagcaa cctggagatc ttctgcccgg
     3301 gccagagcct gaaactcatc ttttgaccgc ccaagggtat attgcccacc agagttaacc
     3361 tttgtggcgg agtcctgacc ctggttttgc attgcccccc ccttgccaac cgtggagtcg
     3421 cgagcttcgg tacctgctaa taccaataaa ttaccgccat tttccagcaa catattggtc
     3481 gctaaattgc cggaaatgga aaaagtgccg tactggtgag taccgctgat ttcaataccg
     3541 ttagccgtgc tcgtctggag agcggcaccg ctgttctgga cgatatctgt cgctttgcca
     3601 ttatcgttga ctgtcagcgt accgccttca ttgatcgttg tttttattgc ctctccgtta
     3661 gctgaaactg tttgtattcc gccgtcgtta attgttgtct cattcgccac accctcgaca
     3721 atttgttcac cgccggtgag cgtcgtgcct gtcgcagtgg cttttgtttt gacgatctcc
     3781 cgtccgcccg tattgacctg tgttttgtca gaagaggtgt ctgactccac ggttaacact
     3841 ccgccatttg ccagcaggat attgttcgcc gcaccctgct cgatgctgaa cgcgacgcca
     3901 tccgcgcgtg ttcctgtgac ccgcgtcgcc ctggtggttg caaccaacgc gccctggcta
     3961 ctctgctgta tccccgttgc gctgcctttc tcccgcacat cgagtgtgcc gccatcatta
     4021 agcaccgagt tttcagccag accgccctca ttaactatct gtgaaccgcc attaataatg
     4081 gaaccttccg ctgtcccgtt tgccataatt tgttgtaggc cagagacgat atcggtattg
     4141 atcgccttac cataattctg aacggtttgc gtgccaccat tgatgtgtgt tttctctgtt
     4201 gacccaccat caacaatttg ttcaccactt tcgatatttg cttcagtggc taaaccatat
     4261 accgtttgct tgccaccttt gatatttgct ttatcagaag tggcactggc atatattgtt
     4321 tgggtgccag cactattgag tacagtgcca acatcttttc cataaacatc cattttgccg
     4381 ttggcattaa taatcgtgtc aactgcccgg gaaccagtga cgactgttaa tgagccagcg
     4441 ttttccagca ctacattttt agcttctgaa ttcctgatgt agaaagcatc accataactg
     4501 ttggttcctt cgataagtgt tccggaggtc gtggaagcaa ttaatgcgcc gccggattgt
     4561 tgctcaacat gcttagcctc accaccgtcc tgaacctcca gaacgccgcc attattaagt
     4621 ctggtgctat ctgttttagc ctccttctgg acaatcagct taccgccagc atcaatggta
     4681 gtatttttcg ccgaggtttt agccactacc gtcagttcgc cggtattttc cagcacaaca
     4741 taattagcct cccctccggt aatagtgaag tgagagagct tgttgtatcc ttcgatatca
     4801 gtccctgcac ccgtgttggc aactaaagca ctgcccgtct cctggttaac cccatgtgca
     4861 ataccgccgg tatagacaat cagcgagcct ccggcgctaa tattgctgcc aattgccgta
     4921 ccatctttct caacaacctg ccggctcccg gaggatataa ttgttgtgtc agctttcccg
     4981 ccgcttttga tattttgcgt tccgctgttg atattggtgc ctgtggctat gccataatta
     5041 ttaatattct gtgtgccacc attaattatg gtatttgtcg cgtttcctgc aacatccata
     5101 accccgccat tatctattcg ggtcgcatca gctttagcat tggttaaaac tgacattgtt
     5161 cctttatctt taataatcgt cttgtttgcc gaaccatatg cgtttatgtc taaatgacca
     5221 ccgttttcca gcaacacatt gtctgccacg tgattgtgga tggagaatgc accttcacta
     5281 ttcgtaccgc tcaccgtcgt accgttagtg ttagttttta aaattgcacc atcgtgctgg
     5341 gtaacatttg ttgccgtacc accactaaca tcaagcacgc caccggaata aacttcaata
     5401 acatccgagg agctggtgtt atcaataatt tgcgtaccac cagaatagat ctgagtattt
     5461 tttgccgttg acttactatt cagagactga gttccgcctt caatcgtcgt gtccagcgca
     5521 cggctctcat atactctttg ctcaccgcca tttttgatgg tggttgtttc tactgtgctc
     5581 tgttcaacat actgtcgacc accatttatg gttgtgttcg ttgccagact tccttgtact
     5641 acgtcctgag agccagactt atttatcgtt gtaccatcag catgcccttg aacttttact
     5701 atctggctac caccatcaat gagtgttcca ttcgcactcc ctccctctac gcgtgaagca
     5761 ccgcccttaa ttgtcgttcc attgctgata ccccctttat aaacgtcctg attgccactc
     5821 tcgattatcg tacctgtgga aatacccccg tcatgaattg actgtctgcc cccgttaatg
     5881 gtggtattat tagactgccc cacataattg ttatgccttc ctacatcttg atatccacca
     5941 gattcaataa gacttccatt agataccccg ccataaacat tctgctggcc atggttgata
     6001 atatgagtgt tatttgttgt acctcgttca tctactcgtt ggctgccatc tacagtctca
     6061 tcgtttacca caccaataac atcaggagtg aaggcagcca tcccgggcgg ggcatatatc
     6121 aaggcagata tcaataagga aagtactgag cgacgacaat aatagggact ggtcctgttc
     6181 ataaatttca tcctctgaaa agtgaatact gagtagcgtt taagcgacct tagctttgct
     6241 gcaacatcag cccacaggca ccagaccagg ggattcatcc tgaagagaca gcgcaagtgt
     6301 attgtgttca ccgctcatca aagacatcat gatgaaatga tgatattccg cataagaatg
     6361 aggcattttt taaacgcagt gcgctgaagt gtggttggat aaaaaagtca atccattcag
     6421 gaaatacggg cgtattcttt tctttcgaca atgaggccgt tggcaaaata aaatgattta
     6481 cataatcgtt tctgatgaat atcttctgct cacataaaaa tcacacaata actttgagat
     6541 cgcagattgt tttactttta cagcattcgt ccccccattg ttgggcaaat atagattggg
     6601 ccagagcacg aaagttaata ccacgttcgc acagctcctc caacagcacg acaagatgcc
     6661 acatactgcg ccccagtcgg ttcagtttac agactagcag tgctttccct gccgatgatg
     6721 tcctgagcag ttttttcagt ccggacctcc tgccacggtt ttacgttgaa gcggtaaccc
     6781 tgagcacgca gttcttcagt caggcgtggt gcaccgtaac gctgttattg ctgggtgaga
     6841 tcaaaaaact ttcaggcagc taaggaaagt tgaaccagac aatagatgaa atatttcaac
     6901 caattacagc accaattcag tcactgccag cccaccaaat aaatcaaggg gttacatgaa
     6961 aacgtagccc ctttttcttt ggtagtgaca ctaaaatgga tgtagtgtga agaataatcc
     7021 cgtttactca atcaataata catattgttt caatctacgt tattatctct ttataaaaat
     7081 tgccatttat ttatcattga aaactgcttt tagaacttta tacaagagga ctattcacaa
     7141 caggactatt ctcaatggga gcatcctcag gggagttatc atcaaagtca tcatccataa
     7201 ataaaatatc atcgaatggt gccacacccg tgatcatttt tattttttta ttacgatcag
     7261 ataagactcc atttaaaccg ttttcgctta caggttttaa tgatttttca taactctcat
     7321 ggttagcagg cgtattaaaa atacacggag tatcaacatc aaacaatgac attccccagt
     7381 tcacatattg aatatcatag ttactgaagt tctgtccaga aaagaagcat cccttaaaat
     7441 ccaatccacg tagattatat aagccaccct cttctttttg gagagtaatg ttaattttgg
     7501 ctatctcccg gacatcatcg ccatttttgt atttgaatac cgtttcaaga tgttctccag
     7561 acagcttgac ttctggaaat aatttgaaat caaagcctac attattatgt gtcaacgtag
     7621 atgaacaaaa gatggaaaaa gcttgcagtg ctgaattata gctatcgatt ttatcttgag
     7681 gctgcgctct tggtaaaaac gtatagcaa
//
LOCUS       DBFIFM010000205         7612 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.205, whole genome shotgun sequence.
ACCESSION   DBFIFM010000205 DBFIFM010000000
VERSION     DBFIFM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.205"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(63..1949)
                     /gene="prpE"
                     /locus_tag="N7C74_003964"
     CDS             complement(63..1949)
                     /gene="prpE"
                     /locus_tag="N7C74_003964"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HGU2433742.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLNQIRQ
                     AMEE"
     gene            complement(1989..3440)
                     /gene="prpD"
                     /locus_tag="N7C74_003965"
     CDS             complement(1989..3440)
                     /gene="prpD"
                     /locus_tag="N7C74_003965"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HGU2433743.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVSIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPVFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            complement(3474..4643)
                     /gene="prpC"
                     /locus_tag="N7C74_003966"
     CDS             complement(3474..4643)
                     /gene="prpC"
                     /locus_tag="N7C74_003966"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HGU2433744.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVESKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(4799..5689)
                     /gene="prpB"
                     /locus_tag="N7C74_003967"
     CDS             complement(4799..5689)
                     /gene="prpB"
                     /locus_tag="N7C74_003967"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HGU2433745.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYHYEEKLDDLFVRSQAK"
     gene            5928..7514
                     /gene="prpR"
                     /locus_tag="N7C74_003968"
     CDS             5928..7514
                     /gene="prpR"
                     /locus_tag="N7C74_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HGU2433746.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKIPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
BASE COUNT         1674 a         2150 c         2076 g         1712 t
ORIGIN      
        1 gcgtcgcatc aggcatctgc gcacaactgt cggatgcgcc acgcgacgca tccgacaata
       61 ccttactctt ccatcgcctg gcggatctga ttcaacgacg caggatcatc aatggtcgtc
      121 agatccccag gatcgcgtcc ttcgcaaatc gcctggatcg tgcggcgcag catttttccg
      181 gatcgcgttt ttggcaattg cgagacaaac cagacgtgcg ccgggcggcc aaagttgcca
      241 atctggctgt ccaccagcgc cataatcgcc ttctcttgcg aatgcgccac atcacgatct
      301 tccagactgt cgctctcttt cggaatgaca aacgccaccg ccacctgccc tttcagcgca
      361 tctttcaccc caaccaccgc cacttcggca acgcccggat gactggagat actctcttca
      421 atctcgcgcg tccccagccg atgcccggca acgttaatca catcgtcagt gcgcccgaga
      481 ataaagtgat aaccgtcagc gtcacggatg ccccagtcaa aggtggcgta caccgggcgg
      541 gaaaacagcg accagtacgt cttcacaaag cggtcgtcgt cgccccagat ggtctgaata
      601 caccccggcg gcagcggccc ttccaccacc agcatccctt tctcgttgac gccacacggt
      661 tcgccggtga cttcattaag caactgcacg ttatagccat acatcggcac gccggggctt
      721 cccagacgcg tcggcctgtc gtccagaccg cgagcaatcg ccataatcgg ccagccggat
      781 tcggtctgcc agtagttgtc gatgaccggc acatccagcg tattgctcac ccaactggcg
      841 gtcggctcgt ccagcggttc tccagccaga tagagcactt ccagcgacga gagatcgtgt
      901 ttgcgaattt cagcggtagg gaattttttc agcacgcgaa tggcggtcgg cgctgagaac
      961 atccggctaa cctgatattt ctcgacgatt ttccaccaca cgccgcagtc cggccaggtc
     1021 ggtaatcctt cgtaaacgat agtcgccatc cccgccagca gcggcgcgta gacgatatac
     1081 gaatgcccca ccacccagcc gatatccgaa gcacaaaaga acacaccgcc cgctttgccg
     1141 ccaaaaatgg tgtccatcga ggtcgccagc gccaccgcat atccgccaac gtcacgctgc
     1201 acgcctttag gtttgccggt cgtgccggag gtgtagagaa tgcaggaggt ttcgttggat
     1261 tccagccacg ccaccggtac ccgcgcgccg atgtgttgat ggcgcaacga cgcgaaatcg
     1321 acatcccgcc cgctgacgcg tgccattttc gccagcccgc gatccaccag caaaacatgg
     1381 cgtggctggt gctgcgcctg acttatcgca tcgtcgagca attttttata gggaatgatt
     1441 ttgccaccgc gcgctccggc atcagccgag acaatcagca ccggtttagc atcatcaatt
     1501 cgcgccgcca cgctgtgcga ggcaaatccc ccaaacacca ccgagtgaat agcgccaatg
     1561 cgcgcgcagg ccagcagagt aatatgcgct tcggcaatca tcggcatata caccagcacc
     1621 cgatcgccac gctgcacgcc cagcgagcgc agcattgacg ccaccgcgtt tacttcgtca
     1681 tgaagctggc gaaaggtaaa ggtgcgctct tcttctgttt ccgaagagac ggcaatcagc
     1741 gccagcgcct ctggttgttt ctccagccag cggtcgatag cgttgtggca caagttggtt
     1801 cggccttcac aaaaccaacg ggcaaacggc ggattgctgt gatcgagcgt ttgcgtaaag
     1861 ggcgtctgcc agtcaatacg ccgggcctgc tcggcccaga actgctccgg ttcgttaatc
     1921 gaacgctgat aaaattcgct aaaagacata atgctctcct gttgaactta cgccttaccg
     1981 ccgtttactt aaatgacgta caggtcgaga tactcattga ccggcatctg ttccaggcga
     2041 gctctgtcga gagaaacctc cagaatgcgc tgctgttggc gagtcgggaa ctggcgcgcg
     2101 agattgattt tgaatttatc gaccagtttc ggaataccat cctgacggcg gcgagcatga
     2161 ccaatggggt actccaccac cacttcttca aatcgtgtgc cgtcggtgaa ctcaagggta
     2221 atggcattgg cgatggcgcg tttttccggg tcgtggtagt cagcggtaaa taccggatct
     2281 tcaaagcaat tgatcttctc gcgcagggcg tcaatgcgtt tatcttgcgc aacgttgtcc
     2341 tcgtaatctg ccgccgttaa gcgcccgaat agcagtggga tcgccaccat gtactgaatg
     2401 cagtggtcgc ggtctgccgg gttattgagc ggcccctttt tgtcgatgat gcgaatacag
     2461 gcttcgtggg tgcggatgga cactttttcg atatctgccg ccgttttgcc tgctgcctgc
     2521 atctgttcat agagcgtcat cgctgcttca actgccgtct gggagtggaa ctccgccggg
     2581 aaggagattt tgaacagcac attttccatc acgtaagaac cgtacggacg ctggaagcgg
     2641 aacgattcac ctttaaagga gacgtcgtag aagccccaca caggcgcagt cagggctgac
     2701 gggtaaccca tttcgcccgt tttcgccatc agtgccagac gtaccgcgcg ggaagtggca
     2761 tcgcccgccg cccaggattt acgcgtgccg gtgttcggcg catggcgata ggtgcgcagc
     2821 gactgaccgt ccacccacgc cagcgaaaca gcgttgagaa tttcctcgcg ggtcaggcca
     2881 agcatttcgg cgaccacggc ggtggaagcc actttcacta acagaacgtg gtcgaggccg
     2941 acgcggttaa aggagttttc cagcgcgatg cagccctgaa tttcatgggc tttaatcatt
     3001 ccggtcagca cctgtttcat ggtcaacggc gctttgccgc tggcgaccgc gttgcgtgaa
     3061 agccagtccg ccgttgccag aatgccgccg agattgtcgg aaggatggcc ccactctgcc
     3121 gcaagccagg tatcgttgaa atcgagccag cggatcatcg cgccgatgtt aaatgccgcc
     3181 tggacggggt cgagctggaa ctgagttccg gggacgcgca cgccgttagg tacgacggtg
     3241 ccggggacaa ttggccccag cagtttttta caggctgggt attcgagagc ttcaagaccg
     3301 cagccgagcg tgtcgagcag acagtaatgt gcggtgtcgt aggctacttt ggagctgatt
     3361 tcgtagttca tgacgtaatc gacgatatca acgatttcac gatcaaattc cgggcggatg
     3421 ttgttgattt gagctgacat tgggtacgtt tccttattgt tattcgtaga ggtttactgg
     3481 cgcttatcca gcgcgacaaa ctggcggtct tccggtccaa cataattggc ggaaggacgg
     3541 ataattttgt tgtcctgacg ttgttcgata atgtgcgccg cccagccggt gacgcgggcg
     3601 ataacaaaca gtggtgtgaa catctcggtg ggaacgccca tcatgttgta ggaaacagcg
     3661 gagaaccagt cgagattggg gaacatcttt ttgctctccc acatcaccgt ttccaggcga
     3721 tcggcgatgt tgtacatctt cagcgagccg ccttcctgcg agagctgctt cgccacacgt
     3781 ttaatcacct ggtggcgcgg gtcggcgatg gtataaaccg gatgaccaaa accaatgacc
     3841 acttctttgc tttccacccg cttgcggata tcggcttcgg cttcgtccgg cgtttcgtag
     3901 cgttgctgga tctccagcga cacttcattc gccccgccgt gcttcggccc gcgcagtgca
     3961 ccaatcgcgc caataatcgc ggaatacata tcagagccag tgcccgcaat cacccggctg
     4021 gtaaaggtgg aggcgttaaa ctcgtgttcg gcgtacagca ccagcgaaat atgcatcgcc
     4081 ttttcccagc tttgcgacgg cttttcgccg tgcagcagat gcaggaagtg accaccgatg
     4141 gagtcgtcat cggtttccgg ctggatgcgt tcgccgttgt ggctgtagtg ataccagtag
     4201 agaagaatcg agctaagtga cgccagcagt ttgtcggcaa tatcccgcgc gccagagacg
     4261 gtatgcccct ctttttctgg cagcgtgcag ccgagcgcgg aaacgccggt gcgcataaca
     4321 tccatcgggt gtgacgccgc cggtaaggct tccagcacgg tacgcacgtt agccggtaaa
     4381 ccgcgcaggg ctttcagttt cgttttgtag gcggcgagtt cgtcacgggt cggcagtttg
     4441 ccgtggatca gcagatgcgc cacttcttca aattcgcaat gttccgccag atcaagaata
     4501 tcgtagccac ggtaatgcaa gtcattgcca cttttaccca cggtgcagag cgccgtattg
     4561 cccgccggaa cgccagaaag cgccacagat tttttcggtt taatgacatg ggtactgttt
     4621 tgcaggatcg ttgtgtcgct catattgtcc tcgtcattgt tatcatttgt agggtcgaga
     4681 ttgttgtcgg atgcgacgcc atgcgtctta tccgacctac agttcacccc gtaggcctga
     4741 taagtcgcgt cagcggcgca tcaggcatcg gttgtcgaat acaaccaacg ggcgtttttt
     4801 atttcgcctg gctacggaca aacaggtcgt cgagcttctc ttcgtagtgg tagtagttga
     4861 tgctttcgta cagctcgttg cgggtctgca tggtgtcgat gacgcttttc tgcgtgcctt
     4921 cctggcgcag gacgttgtag acatgttcgg cggcgcggtt catggcgcga aacgctgaaa
     4981 gcgggtacag cgccatcgcg acatgagcgc tgcgtaattc gtcggtggta aacagcggcg
     5041 tggcgccaaa ttcggtaatg ttggcgagga ttggcacctg caccgcatcg gcaaactggc
     5101 ggtacatggc gagttcggta atcgcctccg ggaacaacat ctcggcaccc gcttcaacat
     5161 aggcctgcgc acgctcgatc gccgcatcca gcccctctac cgccagcgca tcggtgcgcg
     5221 ccatgatcac aaaatcagga tcggttttcg catccaccgc cgcgcggatc cgatccacca
     5281 tctcctcttt cgagacgatc gctttgttcg ggcgatgacc acagcgtttt gcaccaacct
     5341 gatcttcaat atgcaatccc gccgcaccgg ctttaatcat cgatttcacc gtacgcgcaa
     5401 cgttaaaagc agaagaacca aaaccgatat ccgcatccac cagcagcggc agcgaacaaa
     5461 cgtcggtgat acggcgaata tctgtcaaca cgtcatcaag agtagaaata ccgagatcgg
     5521 gcagccccag cgaccctgcc gccacgccac cgccggagag ataaattgcc tgatatccgg
     5581 cacgctgcgc caacagcgca tggttggcgt taatggtgcc aacaatctgc aatggatttt
     5641 ctttagtcag tgcagcgcga aacgctttac ctggagagtg tagagacata gcccatcctt
     5701 tgttatcaac ttgttatttt cgttgataac gacaaagcaa ggggtgtgcc acaattgcct
     5761 taataaaatt aaatctttaa tttcaatttg ttagagataa agtcatgaac taatccgcaa
     5821 atatgcgttt cagttaacgt ttcaggcaat gtttcaccgc gtttcattgc aacaattatg
     5881 aaacaagact aaacccaata ttcggtatct taactttgcg gtgcgctatg gcacatccac
     5941 cacggctgaa tgacgacaaa ccggttatct ggacggtatc tgtaacgcgc ttattcgagc
     6001 tgtttcgcga tatcagcctc gagtttgatc acctggcgaa cattacccct attcagcttg
     6061 gctttgaaaa agcagtgacc tacatccgca agaaactggc aaacgaacgc tgtgacgcca
     6121 tcatcgccgc tggctctaac ggcgcgtacc tgaaaagccg cctgtcagtg ccagttattt
     6181 tgattaaacc gagcggctac gatgtgttac aggcactggc aaaagccgga aaactcacct
     6241 cttctatcgg cgttgtcact tatcaggaaa ctattccggc actggtggcg tttcaaaaaa
     6301 cctttaattt gcgcctcgat caacgtagct acattaccga agaagacgca cgcgggcaga
     6361 ttaacgagct aaaagctaac ggcaccgaag cggtggtcgg cgcggggctg attaccgatc
     6421 tggcagaaga agccggaatg accggaattt ttatctattc cgccgccacc gtgcgccagg
     6481 cgttcagcga tgcgctggat atgacgcgca tgtcgttacg ccataacact cacgatgcca
     6541 cccgcaacgc cctgcgtact cgttacgtgc tgggcgatat gctcggtcaa tcaccacaga
     6601 tggagcaggt gcggcagact attttgctgt atgcccgctc cagtgcggcg gtgttgattg
     6661 agggggaaac ggggacgggc aaagagctgg cggcccaggc aattcatcgg gaatattttg
     6721 cccgccacga tgcgcgacag ggcaaaaagt cgcatccgtt tgttgccgtc aactgcgggg
     6781 cgattgccga atcgctgctg gaagcggagc tgtttggcta tgaggaaggg gcgtttaccg
     6841 gctcgcgacg cggaggtcgc gccgggctgt tcgaaattgc ccacggtggc acgctgtttc
     6901 tggatgagat tggcgaaatg ccgctacctt tgcagactcg cctgttacgg gtgctggaag
     6961 aaaaagaggt cacccgcgtc ggcgggcatc agcctgttcc ggtagatgta cgggtcatta
     7021 gcgccactca ctgcaatctg gaagaagata tgcggcaagg gcagtttcgc cgtgatctgt
     7081 tttatcggct gagtattttg cgtctgcaat tgccaccact gcgcgagcgg gtggcagata
     7141 ttcttccgct ggcggaaagc tttttgaaag tgtctctggc ggcgctctcc gccccatttt
     7201 ctgccgcatt acgccaggga ttacaggcaa gcgaaaccgt gctggtgcac tacgactggc
     7261 caggtaatat tcgtgaactg cgcaatatga tggagcgact ggcgctattt ttaagtgtgg
     7321 aaccgacgcc ggatttaacg ccgcagtttt tgcagctgct actgccggaa ctggcgcgcg
     7381 agtcggcgaa aattcccgct ccacgcttgc tgacaccaca acaggcactg gagaaattta
     7441 atggcgataa aacagcagcg gcgaattatt taggcatcag ccggacgacg ttctggcggc
     7501 ggctgaaaag ctgaactgcc gaatgcagcg cgaacgcttt atccggcata aatttcaata
     7561 gatgatcacc gataaactta cgcatcgggg atgtttacat aggattcaga at
//
LOCUS       DBFIFM010000206         7426 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.206, whole genome shotgun sequence.
ACCESSION   DBFIFM010000206 DBFIFM010000000
VERSION     DBFIFM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.206"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(239..724)
                     /gene="sixA"
                     /locus_tag="N7C74_003969"
     CDS             complement(239..724)
                     /gene="sixA"
                     /locus_tag="N7C74_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HGU2433747.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGEFNWQMSPCNLKMAKA
                     I"
     gene            complement(927..3071)
                     /gene="fadJ"
                     /locus_tag="N7C74_003970"
     CDS             complement(927..3071)
                     /gene="fadJ"
                     /locus_tag="N7C74_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HGU2433748.1"
                     /translation="MEMTSAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGIP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVAHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3071..4381)
                     /gene="fadI"
                     /locus_tag="N7C74_003971"
     CDS             complement(3071..4381)
                     /gene="fadI"
                     /locus_tag="N7C74_003971"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HGU2433749.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVVESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQLLAEDNNIRGNSSLADFAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     ADLTLIDMHEAFAAQTLANIQLLGSERFAREALGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4561..4845)
                     /locus_tag="N7C74_003972"
     CDS             complement(4561..4845)
                     /locus_tag="N7C74_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HGU2433750.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5217..6557
                     /gene="fadL"
                     /locus_tag="N7C74_003973"
     CDS             5217..6557
                     /gene="fadL"
                     /locus_tag="N7C74_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HGU2433751.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGKTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRVFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6922..>7426
                     /locus_tag="N7C74_003974"
     CDS             6922..>7426
                     /locus_tag="N7C74_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433752.1"
                     /translation="MLSSISINNTSAAYPESINENNNDEVNGLVQEFKSLFNGKEGIS
                     TCIKHLLELIKNAIRGNDDPDRININNSSVTYINIGSNDTDHITIGIDNQEPIKLPAS
                     YKDKELVRTIINDNIVEKTHDDINNAEFTDGGIDDRSDIGIDSNDTDDIDIDNQEPIK
                     SPANDENK"
BASE COUNT         1945 a         1934 c         1702 g         1845 t
ORIGIN      
        1 agacacgaaa atatcatttc ttttttatta ttaactgttc tattagtttg atgctttgta
       61 aaaatacatt acaacttagt ctcatttcgt cagaaattaa tagggaagca gaggaaaaag
      121 tgcgatgaat tctgtatcga agcgtatcaa ctccgtgaag ggttttgagt gaatttctac
      181 tattgcaaaa agacaaatgc ccgatgtaca gcttacatcg ggcatttttt caaacgaatc
      241 agatagcttt cgccatcttc aaattacatg ggctcatctg ccagttgaac tcgccttttc
      301 cgctttcatc aagcgttacg ctggcaatgg ccgaggtggt aaacataggc ggcgtttcgc
      361 ccggacataa ctcggcgacc agatacccaa ctaacggtaa gtgggagata accaacaccg
      421 aagcaacacc ttcattggtc aacgcctgca aataggcact gaccagacca acatcgccgc
      481 agggcgttaa ctccggcaga acttctgcac tggaaggcag gttcagacaa tccccaactt
      541 cctccagtgt ttgctcggct cgcaggaacg ggctcaccag aacgcgttcg atttccactt
      601 tttgaccttt cagccagttc gccatcaggc gagattcgtc acaaccgtta gtggtcagag
      661 gacgaaccga atcactggcg gcatcgaggg ctgcgtcgcc gtgacgcatg ataaaaactt
      721 gcatattgca ccgcttttgt taaccagatt cgcccgtttt ctttcatctg aaaccagaga
      781 aagaaaacgg tggccggcat tgtgccttat ccattcaccg aatgaaacgc tgttttttac
      841 ctcaatggcg taagtatagt caatatgcgt ttacattttg accaacaatc cgccattcag
      901 cgcggattca tatagctttg accttcttat tgcaggtcag ttgcagttgt tttccaaaaa
      961 ctttccccac gcgcacccat ctcgactaaa cgctcgcaag gggtaaaacg tgaaccatac
     1021 tgcgtggcca gtcgttgcat tattgcaacc acttcgcccg cgccgagaga atcgatatag
     1081 cgaaacggtc caccgagaaa tggcggaaaa ccaatgccaa ataccgcacc aatatccccg
     1141 tcacgcacgc tacggataac ctgctcatca acacaacgta cagcttcatt cagcatcaac
     1201 atcacacacc gttcagcaac ctgcggtgcg gagagtcgcc cctgcccttg tgcgccaatg
     1261 agcgggtaaa tggcaggatc gacctgtttt ttgcttttac gccctttctg accataaaga
     1321 tagaaacctc gaccattttt tctgcctttg cgatcgtcgt tcaaaattga agaaacaaca
     1381 tttgcaggcg cgctaaaacg ttctccataa gcggcttcca gtacaggaat aattttagtc
     1441 ccggtgtcga ttcctacctc atccaaaagt tggattggac ctaccggaaa accaaatttc
     1501 actagcgcgg catcaatgtg cgctacccgt tcaccttcgg tcagcatgcg gatagcttca
     1561 ttaatgtaag gcgctaagat gcgattgacg taaaacccgg ctttgtcacg cacgacaatg
     1621 ggcgttttgc cctgtttttt cgccagtttt accgtagtgg cgatggtttg tgctgatgta
     1681 cccgcatgag gaataatctc caccagcggc attttttcca ccggactgaa gaaatgcagg
     1741 ccgataactt gctcaggtcg cgtggcgtga gcggcgatat caccaatcgg taaagatgac
     1801 gtattcgaag caaagatggt atgagcggcg caattttgct caacttccgc caccatctgt
     1861 tgtttcaatt cgagattttc aaacaccgct tcaataatca gatcgcgatg ggcaaagccg
     1921 cgatagtccg tcgttccgga gattaatgcc agctgtttgt cacgttcgct ggctttgaga
     1981 tgacgacggc gaactttgcc ctccagctga tcccaactgt acttcagcgc atgatttatg
     2041 ccctgcgggt tgatatcttt aattctgacc ggaatccccg ctttacaagc agtgacataa
     2101 gcaataccgc cgcccatcaa gccaccacct aaaatcccca cgctgtttaa tggcgcaggc
     2161 ggcgcatcac tgccgggatc tttcttcact tccgtactgg caaaaaagat actacgcaaa
     2221 gcttgcgatt gtggcgtcat cgccagttcg ccaaacgccc gtgcttcagc gtcatagcca
     2281 ctgctggtgc cctgcgctaa tcccgtttca acaacctcca ggatgcgttc tgtcgccgga
     2341 taattgcctt gagttttgtg ttctgttttc ttgccgacca ttttgaacag cagcgcacga
     2401 cctaacggcc ccgccagaat acgctcgcgt acaggtagag ggcgggaaga tgggcgatcc
     2461 ttctttgcca gctcaacagc ggcttccagc agaatggagt gcggaacaac atcatccacc
     2521 agccccagct ttaatgcctg tttcgcccga agttgttttc cggtgaggat catctctaat
     2581 gctgtgctga cgcctatcag acgcggtaaa cgctgggtgc cgcctgaacc gggtaacaat
     2641 ccaagttgta cttcaggcaa accgagcacc gttttaggat cgtcagtaca aacgcgaccg
     2701 tggcacgcca gcgccaactc cagcccacca cccaggcaag cgccatgaat agccgcgata
     2761 accggaatgg gcaaagcatg aatctccgcc atcaactgtt ggccctgccg cgccagagct
     2821 tccgcttctt gcgccgtttt gcaattgccg atcatgttga tgtctgcgcc agcaatgaag
     2881 ttgtccggtt tagcggagac aaacaccacg cctcgcaact ctttgttttc acggagttgc
     2941 ttaataatgg cgcgcacctg cgaggcaaac tccgccttca gggtattcat tttctcaccc
     3001 ggtacgtcga tggtgatgat agcaatgttg tccagacgaa cattaagagt aaacgctgat
     3061 gtcatttcca ttattccgcc tccagaacca ttgccgcgcc aagcccaccg gcagcacagg
     3121 cggtaactaa accaaatcca ccgccgcgac ggcgaagttc atgcaatgtc tgggtaatca
     3181 tccgcgcgcc ggtcgccgcg aagggatgcc cgtaagcaat cgaaccgcca agcacgttaa
     3241 atttgctatc gtccacttcg ccagtggcat gtgcacgccc cagcgcttca cgagcaaaac
     3301 gttcactacc cagtagctga atattggcca acgtctgggc ggcaaaggct tcgtgcatat
     3361 caatcaatgt cagatctgcc attgtcaaac cggcacgctc cagcgccagc ggtgttgacc
     3421 aggctggacc gagcaacatg tcctgccaga catcgatagc ggtaaatgcg tagctgcgca
     3481 gatatcccag cggtaccagc cctaattctt tcgcccggga ttcagtcatc aggatcaccg
     3541 ccgctgcgcc atcggtcagc ggcgtactgt tcgccgccgt taccgttccg tgtttacgat
     3601 caaacgccgg acgcagcttt gcgaaatcag caagcgagga attaccgcga atattgttgt
     3661 cttccgcgag cagttgttta taaggaggga taaaggcagt catcacctct tctttgagtt
     3721 ttccgtctga ccatgcctga gcggcacgct gatgcgaacg gtgcgctaat gcatcttgct
     3781 gttctcgggt gatgccgtag gttttcgcca tttgctctgc agtgtcgccc atccgcaagc
     3841 cggtagaata ttctgctacc gcaggtggta cgggcattaa gtcgcgcaaa cgcaggcgag
     3901 agaagagttt cagacgctgg ctcattgtgc gggctttgtt gacatcaacc agcacgcgcg
     3961 ccagtttttt actgacgcca attggcaata ccgaagagga atctgcccca ccggcaatcc
     4021 ccgctcggat agtccccgcc atcaggcttt ctacgacgtt tgcaaccgcc tggaaactgg
     4081 tagcgcaagc gcggctgacg ctgtaagcat cggtatgcac attcattcca gtaccgagaa
     4141 caatttcacg cgcaatgttg ggagcttcag gcatttgtac gacctgacca aagaccagtt
     4201 gttcaatcac ttcggcgggg atctcgctgc gtgccagcag ttcgcctacc accatcttcc
     4261 ctaaatcaac cgcgggaatg ccatgaaaag ccgtcgcctg gcgggcaaaa ggcgtacgta
     4321 aaccgctaac aatggcgata cgatcgccct ggcgggtaac cagcggtaaa acctgaccca
     4381 taacactccc ctgtaaaaaa ataaataaag tggtctgacc tgatcatagt cttaaccatt
     4441 tttttacatt tagccaagcg gagaaaaggg aaattgggag ctatgacaca gtgaagacag
     4501 gagaaagaaa agaaaacgcc cctacctgtt gctggcagga gcgtacttat ccgagaggaa
     4561 ttaacgcaga cccagctgga agatcagcat ttcagcttcg caggcaaaag tgaaatcgat
     4621 atccaggcgc acaccatcag actcttcagt gaaagtcggg gagattttgc acggttcgga
     4681 ttccacgcta cgggctttct cagtcagcgc cgccagcgtt tgttctgctt ctgcgcggtt
     4741 tgcaaacacg cggctgtaag acgctgtgca gtcggagttg tccataatgg tgccaacatc
     4801 catacagcag caaaccgggg tttcatcagc actacattta ctcatcgttg atttcctctg
     4861 tatgtgcacc caaggtgcca gataaacgtt gtggatattt tacgcttccg gaaagtgctg
     4921 ctccagttgt taattctgca aaatcggata agtgaccgaa atcacactta aaaatgatct
     4981 aaaacaaaat tcacccgaat ccatgagtgc gccacctcca aattttgcca gctggatcgc
     5041 gtttcttaga tcatatttga aaaaagatag aaacatactt gcaacattcc agctggtccg
     5101 acctatactc tcgccactgg tctgatttct aagatgtacc tcagacccta cacttcgcgc
     5161 tcctgttaca gcacgtaaca tagtttgtat aaaaataaat cattgaggtt atggtcatga
     5221 gccagaaaac cctgtttaca aagtctgctc tcgcagtcgc agtggcactt atctccaccc
     5281 aggcctggtc ggcaggcttt cagttaaacg aattttcttc ctctggcctg ggccgggctt
     5341 attcagggga aggcgcaatt gccgatgatg caggtaacgt cagccgtaac cccgcattga
     5401 ttaccatgtt tgaccgcccg acattttctg cgggtgcggt ttatattgac ccggatgtaa
     5461 atatcagcgg aacgtctcca tctggtcgta gcctgaaagc cgataacatc gcgcctacgg
     5521 catgggttcc gaacatgcac tttgttgcac cgattaacga ccaatttggt tggggcgctt
     5581 ctattacctc taactatggc ctggcaacag agtttaacga tacttatgca ggcggctctg
     5641 tcgggggtac aaccgacctt gaaaccatga acctgaactt aagcggtgcg tatcgcttaa
     5701 ataatgcatg gagctttggt cttggtttca acgccgtcta cgctcgcgcg aaaattgaac
     5761 gtttcgcagg cgatctgggg cagctggttg ctggtcagat tatgcaatct cctgccggga
     5821 agactcctca agggcaagca ttggcagcta ccgccaacgg tatcgacagt aataccaaaa
     5881 tcgctcatct gaacggcaac cagtggggct ttggatggaa cgccggtatc ctgtatgaac
     5941 tggataaaaa taaccgctat gcactgacct accgttctga agtgaaaatt gacttcaaag
     6001 gtaactacag cagcgatctt aatcgtgtgt ttaataacta cggtttgcca attcctaccg
     6061 ccacaggtgg cgcaacgcaa tcgggttatc tgacgctgaa cctgcctgaa atgtgggaag
     6121 tgtcgggtta taaccgtgtt gatccgcagt gggcgattca ctatagcctg gcttacacca
     6181 gctggagtca gttccagcag ctgaaagcga cctcaaccag tggcgacacg ctgttccaga
     6241 aacatgaagg ctttaaagat gcttaccgca tcgcgttggg taccacttat tactacgatg
     6301 ataactggac cttccgtacc ggtatcgcct ttgatgacag cccagttccg gcacagaatc
     6361 gttctatctc cattccggac caggaccgtt tctggctgag tgcaggtacg acttacgcgt
     6421 ttaataaaga tgcttcagtc gacgttggtg tttcttatat gcacggtcag agcgtgaaaa
     6481 ttaacgaagg cccataccag ttcgagtctg aaggtaaagc ctggctgttc ggtactaact
     6541 ttaactacgc gttctgataa cgcgttcgcc tggataaagt cacctgctat gcaggtgact
     6601 ttaactcccc cacttcaccg aaagtagttt cctcattgct tgcctcctga gttttgcaaa
     6661 cagcctgatg gcagctagcc ctttcaaatc agtaagtggt atccacagac aaacctgaag
     6721 ggaaacgcat attttcaggc gttctgctcg tccttctcaa agagtttact tttctgaatt
     6781 tccaggatac tcaaccccct ggctattgtg cgctcataca cgcaaattaa agataggttc
     6841 taaataaatg agcgtttttt gatagtctat ttcattaggt aatatatatt tgtaacaaat
     6901 caatcaacat ggaataaaat catgctatca tctatttcaa tcaacaatac cagcgcagct
     6961 tacccagaat ccatcaatga aaataacaat gatgaagtta atggattagt acaagagttc
     7021 aaaagccttt ttaatggtaa ggaaggaata agcacctgta ttaaacatct acttgagctt
     7081 ataaaaaacg ccatacgagg aaacgacgat cctgatagaa taaatatcaa taattcttca
     7141 gttacttata ttaatattgg ttccaatgat acagaccata ttactattgg tatcgacaac
     7201 caagaaccaa taaaattacc tgcgagttat aaagacaaag aactcgtccg tactatcatt
     7261 aatgacaaca tagttgagaa gactcatgat gatataaata atgctgaatt tactgatggt
     7321 ggtattgatg atcgtagtga tattggcatt gactccaatg atacagacga tattgatatt
     7381 gataatcaag aaccaataaa atcacccgcg aacgatgaaa acaaaa
//
LOCUS       DBFIFM010000207         7379 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.207, whole genome shotgun sequence.
ACCESSION   DBFIFM010000207 DBFIFM010000000
VERSION     DBFIFM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.207"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            59..1513
                     /gene="ygcU"
                     /locus_tag="N7C74_003975"
     CDS             59..1513
                     /gene="ygcU"
                     /locus_tag="N7C74_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HGU2433753.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVNCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            1607..2943
                     /gene="ygcS"
                     /locus_tag="N7C74_003976"
                     /pseudo
     CDS             1607..2943
                     /gene="ygcS"
                     /locus_tag="N7C74_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            2921..3700
                     /gene="ygcR"
                     /locus_tag="N7C74_003977"
     CDS             2921..3700
                     /gene="ygcR"
                     /locus_tag="N7C74_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HGU2433754.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAHGNSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFASE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRIRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAELPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3697..4557
                     /gene="ygcQ"
                     /locus_tag="N7C74_003978"
                     /pseudo
     CDS             3697..4557
                     /gene="ygcQ"
                     /locus_tag="N7C74_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(4705..5280)
                     /gene="ygcP"
                     /locus_tag="N7C74_003979"
     CDS             complement(4705..5280)
                     /gene="ygcP"
                     /locus_tag="N7C74_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HGU2433755.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     QIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(5297..5557)
                     /gene="ygcO"
                     /locus_tag="N7C74_003980"
     CDS             complement(5297..5557)
                     /gene="ygcO"
                     /locus_tag="N7C74_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HGU2433756.1"
                     /translation="MSVARNLWRAADAPHIVPADSVERQMVQRLINACPVGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(5548..6819)
                     /gene="ygcN"
                     /locus_tag="N7C74_003981"
     CDS             complement(5548..6819)
                     /gene="ygcN"
                     /locus_tag="N7C74_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU2433757.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDCATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDTALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPVLLQRPGWYRTWPALMQDISRDLWDQGDKP
                     VPPLRQLLWRHLRRHGLWNLAGDVIRSVRCL"
     gene            complement(6897..7259)
                     /gene="queD"
                     /locus_tag="N7C74_003982"
     CDS             complement(6897..7259)
                     /gene="queD"
                     /locus_tag="N7C74_003982"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HGU2433758.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
BASE COUNT         1748 a         1872 c         2001 g         1758 t
ORIGIN      
        1 ggtttctgtt ttctcttatt aatgattatt ccatttttat tatcaggaag gaattactat
       61 gtctttatct cgcgcagcga ttgtcgacca gctaaaggaa attgttggtg cagatcgcgt
      121 aattaccgat gaaacagtat taaagaagaa cagtattgac cgttttcgta aatttccgga
      181 tattcatggc atttatactt tgccgattcc ggcagcggtc gtaaaactcg gttccacaga
      241 gcaagtatcc cgtgtgctga attttatgaa tgcgcacaaa attaacggtg tgccgcgtac
      301 cggtgcttcc gccaccgaag gtgggctgga aactgttgta gaaaactcgg tggtgctcga
      361 cggttccgcc atgaatcaaa tcattaatat tgatattgag aatatgcagg cgacggcgca
      421 atgtggtgtt ccgctggagg tgctggaaaa cgcgttgcgt gaaaaaggtt acaccacggg
      481 gcattctccg cagtcaaagc cgctggcgca gatgggcggc ctggtagcaa cccgcagtat
      541 cgggcagttc tccacactct acggcgcaat cgaagatatg gtcgttggtc tggaggcagt
      601 gttggcagat ggcaccgtca cacgcattaa aaacgtgcca cgccgcgcgg ctggcccgga
      661 cattcgtcac atcatcatcg gcaacgaagg tgcattgtgc tatatcactg aagtaacagt
      721 gaaaatcttt aaattcaccc cggaaaacaa cctcttctac ggctatatcc tggaagacat
      781 gaaaaccggc ttcaacatcc tgcgtgaaat catggtggaa gggtatcgtc cgtcgatcgc
      841 tcgtttgtat gacgctgaag atggcaccca acacttcacc cattttgccg acggaaaatg
      901 cgtgctgatc tttatggctg aaggtaaccc tcgcattgcg aaggcgacgg gcgaagggat
      961 tgcggaaatc gttgcccgct acccgcaatg ccaacgcgtg gacagcaagc tgatcgaaac
     1021 ctggtttaac aacctgaact ggggaccgga taaagtggct gccgaacgtg tgcagatcct
     1081 caaaaccggc aacatgggct ttactaccga agtgtccggc tgctggagct gcatccacga
     1141 aatctacgaa agcgttatta accgtattcg taccgagttc ccgcacgccg acgacatcac
     1201 catgctgggc ggtcactcct ctcatagcta tcagaacggc accaacatgt acttcgtcta
     1261 cgactacaac gttgtcaact gtaagccgga agaggaaatc gacaagtacc acaatccgct
     1321 caacaaaatc atttgcgaag aaaccattcg cctcggtggt tcgatggtgc accaccacgg
     1381 tatcggtaaa catcgcgttc actggagcaa gctggaacac ggcagcgcgt gggcgttgct
     1441 ggaagggctg aaaaagcagt tcgatcctaa tggcattatg aatacgggta ctatctatcc
     1501 gattgaaaaa taatgtgtca ggcagcgttc cgcgatgatg gggcgttgcc ttttcggctt
     1561 ctcaggcgag aagccgttct tattaccgga caatgaaggg gtaaagatga acacttcacc
     1621 ggtgcgaatg gatgatttac cgcttaaccg ttttcactgt cgaattgctg cgctcacttt
     1681 cggcgcacac ctgaccgacg gttatgttct cggcgtcatt ggttacgcca ttattcaact
     1741 tacgcccgcc atgcaactga cgccgtttat ggcgggaatg atcggcggct cggcgcacct
     1801 tggtttgttt cttggcagcc tggttcttgg gtggatctcc gaccatattg gtcggcaaaa
     1861 aatcttcacc ttcagctttt tgctgattac gctcgcttcg ttcttgcaat tttttgccac
     1921 cacgccagag catcttattg gactgcgcat tttgatcggc attggtctgg gaggcgatta
     1981 ctcagtaggt cacaccttgc tggctgaatt ttccccgcgc cgccatcgcg gtgttttgct
     2041 gggcgcattc agcgtggtgt ggaccgtagg ctatgtgttg gcaagtattg ccggacatca
     2101 ctttatttcc gaaaacccgg aggcctggcg ctggctgctg gcatcggcag ctctgcccgc
     2161 gttgttgatt acgttattac gctggggaac gccagaatcg ccacgctggc tactgcgcca
     2221 ggggcgtttt gcagaagctc acgctatcgt gcatcgctat tttggtcccc atgttttact
     2281 gggcgatgaa gtggcaacgg cgacccataa acacatcaaa accttgttct cttcgcgcta
     2341 ctggcggcgc acggcgttta acagcgtctt ctttgtctgc ctcgtaatcc catggtttgt
     2401 gatttatacc tggctgccaa ctatcgccca gactattgga ctggaagatg cgctgactgc
     2461 cagcctgatg cttaatgcgt tgttaattgt gggcgcgctg ctgggattag ttctgacgca
     2521 cctgctggca catcgcaaat ttttgctggg aagttttttg ctgctggcgg caacgctggt
     2581 agtgatggcc tgtttgcctt ccggcagttc attaacgctg ctgcttttgt tctcttcagc
     2641 accaccattt cggcagtcag taatctggtg ggcattttgc ctgcggaaag ttttcctact
     2701 gacattcgct cgctgggcgt cggttttgcc accgccatga gtcggttggg ggcggcggta
     2761 agtactggcc tgctgccgtg ggtgctggcg cagtggggaa tgcaagtcac cttattgctc
     2821 ctggcggcag tgttgttggc tggttttgtt gtaacctggc tatgggcacc agaaaccaaa
     2881 gcactcccgc tggtggcggc gggaaatgta ggaggtgcga atgaacattc tgttagcgtt
     2941 taaagccgaa ccggatgccg gaatgctggc ggaaaaagag tggcaggcgg cggctcatgg
     3001 taatagcgga ccggatattt cgctactgcg aagtttactc ggtgctgatg aacaggctgc
     3061 cgccgcgctg ctgttggcgc agagaaaaaa cggtacgccg atgtctttaa ccgcgttgag
     3121 catgggggat gaacgggcgt tgcactggct gcgctatctc atggctcttg ggtttgagga
     3181 agctgtattg ctggagacgg cggcagatct gcgctttgcg tcggaatttg tcgctcgcca
     3241 tattgccgaa tggcagcgtc agaatccgct ggatctgata attaccggct gccaaagcag
     3301 cgaagggcag aatgggcaaa cgccattttt gctggcggaa atgctgggct ggccttgctt
     3361 cacccaggtg gagcgtttca ctctcgacgc gccgtttatc accctcgaac agcgtactga
     3421 acatgggctg cgctgttgtc ggattcgctt gcctgcggtg attgccgtgc gtcagtgcgg
     3481 tgaagtggcc ttgccagtac cgggaatgcg ccagcgaatg gctgcgggga aagcggaaat
     3541 tatccgtaaa actgttgctg ccgaattgcc agcgatgcag tgtctgcaac tggcaagagc
     3601 agagcaacga cgcggagcga cgctgattga tgggcaaacg gtagcagaaa aagcgcaaaa
     3661 gctatggcag gactatttac gccaaaggat gcagccatga atatcgcaat tgtgacaata
     3721 aatcaggaga atgccgcatt agcgggctgg ctggcggcgc aggatttcag cggttgtacc
     3781 ctggcgcact ggcaaataga accacagccc gtagtggtgg aacaggtgct ggatgcgctg
     3841 gtggaacagt ggcagcgaac gcctgcgaac gttgttctct ttccgccggg aacgtttggc
     3901 gatgaactgt cgactcggct ggcctggcgt ttacatggtg caagcatctg ccaggtgact
     3961 tcgttagaga ttccaatcgt aagcgtgcgc aaatcccact ggggaaatgc gctaacggcg
     4021 acgctgcaaa cagaaaaacg cccgctatgt ctttccctgg cgcgccaggc gggagcagat
     4081 aaaaatgcca cgttgccctc tggtatgcag caactgaaca tagtcccagg tgcgcttccg
     4141 gactggctgg ttagcataga gaacctgaaa aatgttaccc gcgacccatt agctgaggcc
     4201 agacgggtgt tggtggtggg gcagggcgga gaggcagata accaggaaat cgctatgctg
     4261 gcataaaagc tgggagccga agtgggctac agtcgggcgc gagtgatgaa cggtggcgtt
     4321 gatgctgaaa aagtgattgg tatttccgga catctgctgg cacctgaggt gtgtattgtt
     4381 gttggcgctt ctggcgcagc agcgttaatg gcgggcgtac gcaatagcaa gtttgtggtg
     4441 gcgattaatc atgacgccag cgcagcggta ttttcacagg cagatgttgg cgtggttgat
     4501 gactggaaag tgatgctgga ggcactggtg acaaacattg acgcagaatg ccaataacgt
     4561 gctgccggat gatcctgcct acggttctgg actggggacc tgtaggcctg ataagacgtg
     4621 ttaagcgtcg catcaggcgc tgaatgtcgg atgtggcgtg cggcctacaa atcaaaccaa
     4681 attcaattgg ttacgattat cccgtcaaag caattttttc gctaacgtcc agactccggt
     4741 attcgtggtg gaaagcgcca cgacacccgc gttaatcgca ttacgcgcat cttcttcatc
     4801 gcataccagc ccgccagcaa tcagcggttg gcggattttt tccgtcaccc agcccagcac
     4861 tttgggcatg cagcctggca ggatctcaat acaatccgga ttcgattgcg caacttgctt
     4921 atcaatgttg tgaaacgaaa tcgaatcaac aataaacagg cgatgaatac agaaaaaacc
     4981 ttccgctctt gccgctttta gcatcgatgc tttggtgctg ataattccat ccgcctcggt
     5041 caccagtttc aaaaactgaa tcaccacttc tttatttgac gcgccttcca gcaaatcaac
     5101 atgaataaaa gcatatttcc cagcgttctt aatcttttta acgatttgac taatggtgca
     5161 gatattgccg tacaagacgg aaataaactg gcattcagaa tcgatggcca gttgcaggct
     5221 ggcattatct ttaacggcag caatcaccgg attctggcgg agcaggtgta agaggggcat
     5281 aaattatcct tattatttat ccaaatcggt aggtgatgcc gaatccggaa ggcggatagc
     5341 gccactgttg tagtgtggat tcgtcgcaca gcaaacggca ggtgccacac tccaggcagc
     5401 cgtgataatc aacgcgtaag tcaccttccg gtgtgagcga aaaaagacct accggacagg
     5461 cgttaatcaa ccgttgtacc atctgacgct caacggagtc agccggaaca atgtgcggcg
     5521 catcagcagc gcgccagaga ttacgggcta cagacatcga acactcctga taacatcgcc
     5581 cgccagattc cacaagccat gacgacgtaa atgacgccag agtaactggc gcagcggtgg
     5641 aacaggttta tcaccctgat cccataaatc gcgggaaata tcctgcatta acgcaggcca
     5701 cgtacggtac catcccgggc gttgcaaaag caccggaaca tgctgataac gctgtagaac
     5761 atcccacagc aggctgcgct ctacgttgtg atgataaagc ggaaacagat tttgcggctc
     5821 gcggtgctgg caggcgctta tcagtgtttg tgccgccgcc tgcgcgccag ttagcgccgt
     5881 atccatgccg cgcacagaaa ttccggtatt gacgcaactg cgcaacgcat cgcccaccag
     5941 cagccagccg ttaccggcgt attgtaccgg catactgtgc aagccacctt ctggcaccag
     6001 atgcgcacca tactccagtg attccgtgtt tttgataagc gggcgcactg ccggatgcgt
     6061 tttaaagcga gcaagcagct cgcttgccgg aacacgactt tgcgtaaggg aagagagtgg
     6121 gcaaacaatc cctaacgaga gcgtttgttg attagtataa agaaatgcgc cgccgggtaa
     6181 gtcatcacag attccgccgc tgaacagcaa cgctgcgcct tcgttattct ccagatgaaa
     6241 acgttcttca atagcggatg tttccagcga cagcacttct ttgatcccca acgccatcgc
     6301 ttcgccagca ggacgagtca ctaacccgtg acgttcagcc aggacgctgt tggcaccttc
     6361 tgccagcacc acataacggg cgcggagaat atcgtcacca caaatgacac cacacaccct
     6421 gccgttttct tcatacagtg catccaccgt cgcaccaggg atgcattcga caccttcttt
     6481 ttcggcttcg gcaaccagcc acggatcgaa ccgtgcacgt aatacactcc aggattcacc
     6541 gccgggctgt aagctggaaa acgtcgttgc gcaatccggc gttaacaggg aaaggctttc
     6601 gtgagtgatg cgtcgttcaa gaggcgcggt cagatgaaat tgagggagga gttccgcgag
     6661 tgcatgggta tataaccgcc cgccggaaag atttttgctg ccggggattt cagcgcgttc
     6721 cagtaacaaa acggataaac ccgctcgcgc gcagcgtaac gcgcaagcgg tgcctgcaat
     6781 accggcacca ataataataa tgtcgcagtc gtcttccatg tcatgcccca gatatcctga
     6841 acacaacacc ctaacatagc gttacttaag ggaaattgac cgccgccaca ctcttatcat
     6901 tcgccgcgat agatacaacc agcggtgcag gtttctttta ccatcaccgc acttaacagc
     6961 ggcacaacgg gtttaacctg atcccaaatc cattttgcta aaacttcgct agttgggttt
     7021 tccagacctg gaatatcatt gagataatgg tgatcgaggc gctcataagt tggcttaaac
     7081 gccgctttta gttcagcgaa atcgataatc cagcccgtat gcggatcgac ttccccggta
     7141 atttccagtc gcaccataaa ggaatgcccg tgcaggcgac cacatttatg cccttccggg
     7201 acgtgtggta agcggtgagc ggcttcgaag gtgaaatctt taaataacgt ggtggacatc
     7261 ataatttctc tacaggacga taaaaccgcc gtagagtacc ggaaaggctc ttttttcgca
     7321 ttatctaaaa cgactttgtt gcgcaaaatc gctgatttat cttaatgatt ggctaaatt
//
LOCUS       DBFIFM010000208         7369 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.208, whole genome shotgun sequence.
ACCESSION   DBFIFM010000208 DBFIFM010000000
VERSION     DBFIFM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.208"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            35..484
                     /gene="alaE"
                     /locus_tag="N7C74_003983"
     CDS             35..484
                     /gene="alaE"
                     /locus_tag="N7C74_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HGU2433759.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(521..865)
                     /gene="ygaC"
                     /locus_tag="N7C74_003984"
     CDS             complement(521..865)
                     /gene="ygaC"
                     /locus_tag="N7C74_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HGU2433760.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1017..1346
                     /gene="ygaM"
                     /locus_tag="N7C74_003985"
     CDS             1017..1346
                     /gene="ygaM"
                     /locus_tag="N7C74_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HGU2433761.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(1496..2830)
                     /locus_tag="N7C74_003986"
     CDS             complement(1496..2830)
                     /locus_tag="N7C74_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGU2433762.1"
                     /translation="MPRYQDIARQLKTVIEQGKLKPGARLPSSRTWSQELGVSRSTVE
                     NAYAELVAQGWLVRRGQAGTFVSEQIHPQQSAVDVVAFAGESQQPLPFQMGLPALDLF
                     PRELWARVMGRRLRTQTRFDLALGDVCGEAALREAIVDYLRVSRGIDCQPEQVFITHG
                     YAASMALILHALAQPGDGMWMEDPGFPLIRPIVTRHGVEILPVPVDDNGLDVTSGIQN
                     YPDARFALITPAHQSPLGVALSLARRYQILEWAERSQAWIIEDDYDSEFRYHGKPLPA
                     LKSLDAPQRVIYAGTFSKALFPALRCAWLVVPVEQISQFRQQASLEPCAVPVLWQNTL
                     ADFIREGHFWRHLKKMRQHYAQRRQWIEQALTQQGFQVVPQKGGIQMVIKLAGNDVTF
                     VHKANAAGLAVQALSDWRIRSSEDGGLLLSFTNIVSESMARQVAQQLRSALN"
     gene            2918..3349
                     /locus_tag="N7C74_003987"
     CDS             2918..3349
                     /locus_tag="N7C74_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HGU2433763.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELIYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTDIART
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            3558..3803
                     /gene="nrdH"
                     /locus_tag="N7C74_003988"
     CDS             3558..3803
                     /gene="nrdH"
                     /locus_tag="N7C74_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HGU2433764.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3800..4210
                     /gene="nrdI"
                     /locus_tag="N7C74_003989"
     CDS             3800..4210
                     /gene="nrdI"
                     /locus_tag="N7C74_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HGU2433765.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4183..6327
                     /gene="nrdE"
                     /locus_tag="N7C74_003990"
     CDS             4183..6327
                     /gene="nrdE"
                     /locus_tag="N7C74_003990"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGU2433766.1"
                     /translation="MATTTAECLTQKTMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTGKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6337..7296
                     /gene="nrdF"
                     /locus_tag="N7C74_003991"
     CDS             6337..7296
                     /gene="nrdF"
                     /locus_tag="N7C74_003991"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGU2433767.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEYEDWNF"
BASE COUNT         1814 a         1904 c         1857 g         1794 t
ORIGIN      
        1 acatcccatt acgcttttat taaggagcat tagcatgttc tcaccgcagt cacgcttgcg
       61 tcatgcagtt gcagatacgt tcgcgatggt tgtttactgt tctgtcgtga acatgtgtat
      121 tgaagttttc ctctccggaa tgagcttcga acagtctttt tattccagat tggtagcgat
      181 tccggtgaac atcttaattg catggccata cggtatgtac cgcgatctgt ttatgcgcgc
      241 ggcacgcaaa gttagcccgt cgggctggat aaaaaatctg gcggatatcc tggcttatgt
      301 gacgttccag tcaccggtgt atgtggcgat cttgttagtg gtgggcgcag actggcatca
      361 gattatggcg gcggtcagtt caaacatcgt tgtttcgatg ttgatggggg cggtttatgg
      421 ctacttcctc gattattgcc gccgactgtt taaagtcagc cgttaccagc aggtaaaagc
      481 ctgactcttc ttttcgcgac tggcatcgcc agtcgcgtct ttaactggct tcgccaaata
      541 aaccattcaa ataacgttca agcgcaacac gcgaactaaa gccatgcggg ataccgtaat
      601 gctcgtgtcg ctcgcctgct aaatagagcg gaaattgctg gtaatgatcg caagttttaa
      661 tatcggaagc gggttcagcc agtgtcgaac tgcgttcttt taatgttgga tgaattacca
      721 gagcggtacg tcccattcgc gcttcgcgat taacatagac ataattttcc ccacggcggt
      781 aaccatacgc tttctgggtt accacatcga cagtaaaacc gactttttca agtacgcgcg
      841 ccacctcgtc tggtcgtaaa tacatatttg atcctcgtta ttatcaaccg cgggcctacc
      901 ttacctgatt gcgcattcac aacgctttca gaaaagtcca taaagccgcg atgaacctca
      961 gttaagaaaa ttatggtcta cactgaaaat tacatcgaat tctaatgggg gatcatatgt
     1021 ttaaccgacc gaaccgcaac gacgttgatg atggcgtgca ggatatccag aatgatgtca
     1081 atcaattagc tgacagcctc gaatctgtat tgaagtcctg gggcagcgac gccaaagggg
     1141 aagctgaagc cgcacgcagt aaagctcagg cattgctgaa agaaaccagg gcgcgaatgc
     1201 atggtcgtac tcgcgtccag caagccgcgc gcgatgccgt aggctgcgcg gattcttttg
     1261 ttcgtgaaag accctggtgt agtgtgggaa ctgctgctgc ggtaggtatt tttatcggtg
     1321 cgttgttgag catgcgtaaa tcgtagtgag acgatgacaa gcagtagtct gacttctgaa
     1381 aagtactcag tgcgtcaacg ccttgtccgc ctacaggcct gaaaaacata gcgcatcagg
     1441 ggattttgcc ctggtcacca gtcttatagg accccgaagc ctggcttcgg ggttattaat
     1501 ttaaagcact gcgcaattgc tgtgctacct gtcgcgccat actttcgcta acgatattcg
     1561 taaacgagag caataatcct ccgtcctcgc ttgagcggat acgccagtcg ctaagtgcct
     1621 gtacggcaag accagcagca ttggctttat gcacgaatgt cacgtcatta cctgctagtt
     1681 tgatcaccat ctggataccg cctttttgcg gcacaacctg aaatccctgc tgcgtaagtg
     1741 cctgctcaat ccactgccgt cgctgggcat aatgctggcg cattttcttc agatgccgcc
     1801 agaaatgccc ctcacggatg aaatctgcca gtgtgttctg ccacagtacg ggtacagcac
     1861 atggttccag tgacgcctgc tgccggaatt gcgaaatctg ctccaccggc accaccagcc
     1921 acgcacagcg caatgcagga aatagcgctt tgctgaatgt tccggcataa atcacccgct
     1981 gcggcgcgtc gagacttttt agtgccggta acggcttacc gtgatagcga aactcactgt
     2041 cgtaatcatc ttcaataatc catgcctgac tacgctctgc ccattccagt atctgatacc
     2101 tacgcgctaa agagagtgcc acacccagcg ggctttggtg tgccggagta atcagggcaa
     2161 aacgcgcatc aggataattt tgtattccgc ttgtgacatc cagtccgttg tcatcaaccg
     2221 gcacaggtaa aatttccaca ccgtggcgag tgacaatcgg gcgaatcagc ggaaagccgg
     2281 gatcttccat ccacatcccg tctcccggtt gcgccagagc gtgcagaatt aaagccattg
     2341 aggccgcata accgtgagtg ataaagacct gctctggctg acaatcaatc ccacgtgaaa
     2401 cgcgtaaata atcaacaata gcctcgcgca aggccgcctc gccgcagaca tcgcctaatg
     2461 ccaaatcaaa gcgcgtctgg gtacgcagac ggcggcccat cacccgcgcc cacaattcac
     2521 gcggaaaaag atcaagtgca ggtagcccca tctgaaaagg cagcggctgc tgactttcac
     2581 cagcaaaagc cacgacatcc acagcagact gttgcggatg tatctgctca ctgacaaagg
     2641 tacccgcctg tccccgcctg accagccatc cctgcgccac cagctcagca taagcatttt
     2701 cgacagtgga tcgagagaca cccagctcct gcgaccaggt acggctcgaa ggtagccttg
     2761 cgccaggttt cagttttcct tgctcgataa ccgtttttaa ctgacgggcg atatcctgat
     2821 aacgtggcat atggtctgct tttttaggta atttatggct ctctttagca gtccattata
     2881 gtgctacatt gcgggcgtta ataagaggag aaactgtatg actacattac gtcagcctta
     2941 ctacgaactt agcccggcag tgtataacgc actggtgcag gccaaaacgg cactggaaaa
     3001 tagcacgctg gataccacgt tgatggagtt aatttatttg cgcgtctcgc aaatcaacgg
     3061 ctgcgcattt tgtctggaga tgcacagcaa agcattgcgc aaatccggtg tgccacaaca
     3121 caaactggac gccctggcag gctggcgcgt aagccatcat tttgatgaac gcgagcgagc
     3181 ggcgctggca tgggcggaat ctgtgaccga tattgccaga acccatgcgg aagacgaggt
     3241 ttatcagcct ttgcttgagc atttcagcgc agcagaaatc agcgacttaa cgtttgccat
     3301 cgggctgatg aattgtttta accgtctggc cgtttccatg cggatgtaat ttgctgttgc
     3361 cgggtatgcg cccggcaaaa aaacacaaaa tcccctaccc cgttgagcca ttcaccggtg
     3421 gcgtaatcaa tctaaaatta ccatatcttg tgtggttgaa tcttttttca actacatcta
     3481 gtgtctctgt atcaacagag agacaacccg acgcgtatca tcgcgccgta tcttcatttt
     3541 aaacggaaat acgaatcatg cgcattacta tttacactcg taacgattgc gttcagtgcc
     3601 acgccaccaa acgggcgatg gaaaaccggg gctttgattt tgaaatgatt aatgtcgatc
     3661 gcgttcctga agcggcagaa gcgttgcgtg ctcagggctt tcgtcagttg ccggtagtga
     3721 ttgctggcga tcttagctgg tctggtttcc gtccggacat gattaaccgt ctgcatccag
     3781 cgccacacgc ggccagtgca tgagccagct cgtctacttc tccagcagct ccgaaaacac
     3841 gcagcgtttt atcgaacgtt taggtctgcc cgcggtgcgc atcccgctca atgagcggga
     3901 acggattcag gtagacgagc cttacatcct gatcgtgccc tcttacggcg gcggcggtac
     3961 ggctggcgcg gtgccacgac aggtaattcg ctttttaaac gacgagcaca accgggcgtt
     4021 gcttcgcggc gttattgctt cgggtaatcg caactttggt gaggcgtatg gccgcgccgg
     4081 agatgtgatt gcccggaaat gcggcgtgcc gtggctgtac cgttttgaac tcatgggtac
     4141 gcaaagcgat atcgaaaacg ttcgtaaagg agtaaccgaa ttttggcaac gacaaccgca
     4201 gaatgcctga cgcagaaaac gatggattac cacgcgctga atgcgatgct taacctctac
     4261 gatagcgcag gtcgcattca gttcgataaa gaccgccagg ccgttgacgc ctttatggcg
     4321 acgcatgtgc gtccgaacag tgtgaccttc agtagccagc agcagcgcct gaactggctg
     4381 gtcaacgaag gttactatga tgaaagcgtt cttaatcgct actctcgcga ctttgtcatt
     4441 acgctgtttg cccacgcaca caccagcggt tttcgtttcc agacattcct cggggcatgg
     4501 aagttttaca ccagctatac gttgaagaca ttcgacggta aacgttatct ggaagatttt
     4561 gccgatcgag tcacgatggt ggcgctgacg ctggcacaag gcgatgagac gctggcgttg
     4621 caactgacag atgaaatgct gtcaggacgc tttcagccag ccacgccaac attcctcaac
     4681 tgcggtaagc agcagcgcgg cgaactggtt tcctgttttt tgctgcgtat tgaagacaat
     4741 atggagtcga ttggtcgggc ggtaaattcc gcactgcaac tgtcgaaacg cggcggcggc
     4801 gtagcatttt tgctgtcgaa tctgcgggaa gcgggcgcgc caattaaacg tattgaaaat
     4861 caatcttctg gcgtaattcc ggtgatgaaa atgctggaag acgcattttc ctatgccaac
     4921 caactcggcg ctcgtcaggg ggctggtgca gtctatttac atgctcatca tcccgatatt
     4981 ctgcgttttc tcgacacgaa acgggaaaat gccgacgaaa aaatccgcat taaaacactg
     5041 tcgcttggcg tggtgatccc ggatatcact ttccatctgg caaaagagaa tgcgcagatg
     5101 gcgctgtttt cgccttatga cgtagagcga gtttatggca agccgtttgc cgatgtggcc
     5161 atcagccaac actatgacga actggttgcc gatgaacgca ttcgcaaaaa atacctcaac
     5221 gcccgtgatt tcttccagcg actggcagaa atccagtttg agtccggcta tccctacatc
     5281 atgtatgaag acacggtaaa ccgtgctaac cctgtcgccg ggcgcataaa tatgagtaat
     5341 ctctgctcag aaattttgca ggttaacagc gcctcagagt atgacgagaa tctcgactat
     5401 gcccgcacag gccatgatat ctcctgcaat ttaggttcgt tgaatattgc gcacaccatg
     5461 gattcccccg attttgcccg cacggtagag actgccgtgc gcggtttaac ggcagtatca
     5521 gatatgagtc atatccgcag cgtgccgtcc atcgaagccg gaaatgccgc ctcgcacgcc
     5581 atcggactgg ggcagatgaa tttacacggc tatctggcgc gagaaggcat cgcttatggt
     5641 tcgccggaag cactggattt caccaatctc tatttctatg ccatcacctg gcacgcactg
     5701 cgtacctcga tgttgctggc acgcgaacgc ggtgaaacct tcgccgggtt caaacagtca
     5761 cgctatgcca gtggtgaata ttttagccaa tatctgcaag ggaactggca gccgaaaacg
     5821 gggaaagttg gcgaactgtt tgcccgtagc ggtattacgt tacctacccg tgagatgtgg
     5881 gcgcaactgc gcgacgacgt gatgcgctac ggcatttaca accagaatct tcaggcggtg
     5941 ccgccaacag gttctatctc ttatatcaac catgctacgt cgagtattca tccgattgtg
     6001 gcgaaagtag agatacgcaa agagggcaaa acaggacgcg tttactaccc tgccccgttt
     6061 atgactaacg agaatctggc gctgtatcag gacgcttacg aaattggcgc agaaaagatc
     6121 atcgacacct acgcggaagc gactcgccat gtcgatcagg ggctgtcgct gacgctgttt
     6181 ttccccgata ccgccaccac tcgcgatatc aacaaagcgc agatttacgc ctggcgcaaa
     6241 ggtatcaaaa cgctctatta catccggctg cgtcagatgg cgctggaagg cactgaaatt
     6301 gaaggctgcg tctcctgtgc actttaagga atatctatga aactctcacg tatcagcgcc
     6361 atcaactgga acaagatatc tgacgataaa gatctggagg tgtggaatcg cctgaccagc
     6421 aatttctggc taccagaaaa ggtgccgctg tcgaacgata ttcctgcctg gcagacatta
     6481 actgtcgtag aacaacaact gacgatgcgc gtttttactg gcctgacgct gctcgacacg
     6541 ctgcaaaatg ttatcggcgc gccttcgctg atgcccgatg cactcacgcc tcatgaagaa
     6601 gcggtattat cgaatatcag ctttatggaa gcggttcatg cccgctctta cagttcgatt
     6661 ttctcgacgc tgtgccagac caaagatgtc gatgccgcct acgcctggag tgaagaaaac
     6721 gcgccgttgc agcgaaaagc acagattatt cagcaacatt accgcggtga tgatccgctg
     6781 aaaaagaaaa tcgccagtgt gtttcttgaa tcttttttgt tctattccgg tttctggctg
     6841 ccgatgtatt tttccagccg cggaaagctg accaataccg cggacctgat ccgtctgatt
     6901 atccgcgatg aagcagtcca cggttactac ataggctata aatatcagaa aaacatggaa
     6961 aagatatctc tgggacaacg tgaagagttg aagagtttcg ccttcgattt gttgctggaa
     7021 ctctacgaca acgagttgca atacaccgat gagctgtacg ccgaaacccc gtgggctgac
     7081 gatgtgaaag cgtttctctg ttacaacgcc aataaggctt tgatgaatct gggctacgaa
     7141 ccgttatttc ccgcagaaat ggcggaagtg aatccggcaa tcctcgccgc gctttcgccg
     7201 aatgccgatg aaaatcacga tttcttttcc ggttcaggct cctcttatgt gatggggaaa
     7261 gcggttgaaa cagaatatga agactggaat ttctgagggt gttattttca aaaatatcac
     7321 tacccgcgac agggaaataa ttcccgccaa atagcttttt atcacgcaa
//
LOCUS       DBFIFM010000209         7325 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.209, whole genome shotgun sequence.
ACCESSION   DBFIFM010000209 DBFIFM010000000
VERSION     DBFIFM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.209"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            86..316
                     /gene="chaB"
                     /locus_tag="N7C74_003992"
     CDS             86..316
                     /gene="chaB"
                     /locus_tag="N7C74_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HGU2433768.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            477..1172
                     /gene="chaC"
                     /locus_tag="N7C74_003993"
     CDS             477..1172
                     /gene="chaC"
                     /locus_tag="N7C74_003993"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HGU2433769.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     RPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(1216..1569)
                     /gene="ychN"
                     /locus_tag="N7C74_003994"
     CDS             complement(1216..1569)
                     /gene="ychN"
                     /locus_tag="N7C74_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HGU2433770.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLALRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            1755..3149
                     /gene="ychO"
                     /locus_tag="N7C74_003995"
                     /gene_synonym="iatA"
     CDS             1755..3149
                     /gene="ychO"
                     /locus_tag="N7C74_003995"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HGU2433771.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(3150..3800)
                     /gene="narL"
                     /locus_tag="N7C74_003996"
     CDS             complement(3150..3800)
                     /gene="narL"
                     /locus_tag="N7C74_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HGU2433772.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(3793..5589)
                     /gene="narX"
                     /locus_tag="N7C74_003997"
     CDS             complement(3793..5589)
                     /gene="narX"
                     /locus_tag="N7C74_003997"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HGU2433773.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            5928..7319
                     /gene="narK"
                     /locus_tag="N7C74_003998"
     CDS             5928..7319
                     /gene="narK"
                     /locus_tag="N7C74_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HGU2433774.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         1696 a         1805 c         1948 g         1876 t
ORIGIN      
        1 agtctgacat gaaaattctt atgtttttgc aagagtagat attgttgacc acacttaatg
       61 ttcaactttg taaaaggagt caacgatgcc gtataaaacg aaaagcgacc tgccggaaag
      121 cgtaaagcac gttctaccgt ctcatgccca ggatatctat aaagaagcgt tcaacagcgc
      181 atgggatcaa tataaagata aagaagatcg gcgtgatgac gccagtcgcg aagaaaccgc
      241 gcataaagtg gcctgggctg ctgtgaagca tgaatatgcc aaaggggatg atgataaatg
      301 gcataaaaaa tcgtaaaacc ggtagcttag ttaaagctat tcgtgcggtg ttgccttgca
      361 agtggtccgc ggattgcata ttgtcccgtt agtggtttca aaatgagcag taaaaatgtc
      421 cggaagacac caaaaagttg tcgcagggaa gcatgcagtg gcggaggtgt aagaaggtga
      481 taacgcgtga tttcttgatg aatgccgatt gtaaaacggc atttggtgcc attgaagaat
      541 cactcttatg gtcagcagaa caacgggcgg cttcgctggc ggcgacgctg gcttgtcgac
      601 ctgatgagag accggtgtgg atcttcggtt atggatcgtt gatgtggaat ccggcactgg
      661 agtttaccga gtcgtgcacc ggtacactgg ttggatggca tcgcgcgttt tgcctgcgcc
      721 tgaccgccgg gcgcgggact gcgcaccagc cgggacggat gcttgcacta aaagagggcg
      781 gacgtaccac aggcgtcgcc tatcgactgc cagaagagac gctggagcag gaactaaccc
      841 tgttgtggaa gcgcgagatg attaccggct gttatctgcc aacctggtgt cagcttgatc
      901 ttgatgatgg acgcacagta aacgccattg tgtttattat ggacccgcga catccagaat
      961 atgaatctga cactcgcgct caggtcatcg cgccgttgat tgcggcggcg agcggtccgc
     1021 tgggaaccaa cgcacaatac ctgttttcac tggaacagga gcttatcaaa ctgggaatgc
     1081 aggacgatgg gctgaatgat ttgctggtat cggtaaaaaa actgctggcg gagaattatc
     1141 cggatggtgt gttgcggccg ggattcgcct gagtaaactt ccctcatcgt ggggcgtcag
     1201 acgccccctc aaacattaaa atgtgagcac tttatcggct gacagcgtcc attgcgccag
     1261 ttccacaaga gtaccgattt ccaccccatc aatcagagga agtgtactaa tcccgcgccc
     1321 gtcggtacag gttttgcaca atttcaccgg tacattctga gcggtaagga tctccagcat
     1381 ttgctgtatg ttgtagcctt cccctggttt ttgcccgcgc aacccggctg tgaccgcatc
     1441 agacatcagg aacagacgca gagccagatt gctctcctgc tctcgtaacg caatggccag
     1501 ccgcaagctg ttaaacaagg attcgctccc gtaaggtgcg ccattggcaa cgatcacgat
     1561 tttttgcatt atttactcct gtattcaggg aattagacac tcatcttcta tcttactgct
     1621 tctgcagcgt ctgaccaatc ggtcacattt ttaaggattt tcctgaaagc gcgagaaaat
     1681 acgacaaaag ttgccagtaa tcgttattct ttaaggctat gttttttcat tttttaccgg
     1741 aagttaccga cgttttgagc cgtttcgttc ctcgcattat tccgttttat ttactcttgc
     1801 tggcggcagg cggtacagct aacgcacaat ctaccttcga gcaaaaagcg gcaaatccct
     1861 ttgataataa caatgatggt ctgccggatt taggcatggc gcctgaaaat catgatgggg
     1921 aaaaacactt tgctgaaatt gtgaaagatt tcggcgaaac cagtatgaat gataacgggc
     1981 tggatactgg cgagcaggca aaagctttcg cattgggaaa agtccgcgac gcgcttagtc
     2041 aacaggttaa tcagcacgta gagtcgtggc tatcaccgtg gggaaatgcc agtgttgacg
     2101 tcaaagtgga taacgaaggt cattttaccg gcagtcgtgg aagctggttt gtgccgttac
     2161 aagataatga tcgttatctc acctggagcc agcttggtct tactcagcag gatgatgggc
     2221 tggtgagcaa tgtgggcgtt gggcaacgct gggcgcgcgg caactggctg gtgggttata
     2281 acacttttta tgacaacttg ctggatgaaa atcttcagcg agcgggcttt ggtgccgaag
     2341 cgtggggcga atatttgcga ctatcggcaa acttttatca gccgtttgct gcatggcatg
     2401 aacagacagc cacgcaggaa caacggatgg cgcgcgggta cgacctgaca gcccggatgc
     2461 gcatgccgtt ctatcaacac ctcaatacca gtgtcagcgt agaacagtat tttggtgatc
     2521 gtgttgattt gtttaactct ggtacgggtt atcacaatcc cgtcgcgttg agtctgggat
     2581 taaattacac ccctgtgcca ttagtcactg tgacggccca gcataaacag ggtgaaagtg
     2641 gcgagaatca aaataacctc gggctgaatc ttaattaccg ctttggtgta ccgctcaaaa
     2701 aacaactttc tgcgggcgag gttgccgaaa gtcagtcgtt acgtggtagt cgctatgata
     2761 atccgcagcg aaataatcta ccgactcttg agtaccgaca gcgaaaaacg ttaacggtgt
     2821 ttcttgcgac accgccgtgg gatctaaaac ctggcgaaac agtgccgctg aaattacaaa
     2881 tccgcagtcg ttacggtatt cggcaactga tttggcaggg cgatacgcag atattaagtt
     2941 tgacgccggg cgcacaagcc aacagcgcgg agggctggac gctgatcatg cctgactggc
     3001 agaacgggga aggggcgagc aatcactggc gattgtcggt ggtggtggaa gataaccagg
     3061 ggcagcgtgt ctcctccaat gagatcacgc taacgcttgt cgaaccgttc gacgcattgt
     3121 caaacgacga actgcgctgg gaaccgtaat cagaaaatgc gctcctgatg cacccatacc
     3181 gctgcttcca cgcgagactt gagcttcatt ttcttcagca tgtgcttgac gtgcactttt
     3241 actgtgcttt cggtgatgtc caggcggcgg gcaatcatct tgttcggcaa accctgggca
     3301 atcagcttga gaatatcgcg ctcgcgtggg gttaactggt taacatcgcg ctcagtcgtg
     3361 gcacggttag cgcgcaagct ggcggccaga acaggcgtta atgcttcgct taataccatt
     3421 tcgccagcag ctgcctgatg caatgctttc agcagatctt ccggttccat atcttttaac
     3481 agatagccat ccgcgccgcg tttcagtgcg gtgaccacat cttcttcatg gttagagacg
     3541 ctgaatacca caatgcgccc tgagagggac ttttcgcgca gtttatccag cgtttccaga
     3601 ccgttcatgc cgggcatatt gagatctaac aggatcagat cgggatcaag agattccgcc
     3661 agttcaatac cctgttcgcc attactcgct tcgccaacca cggtgatatc tggtgccata
     3721 ctgataagct gttttacgcc agttcgcagc atcgggtgat cgtcaatcag caggatagta
     3781 gccggttcct gattactcat gggtatctcc ttggacgtct gtgaaagttt tttcgggaat
     3841 aaaggtgacc accacttcgg tgccacctga ttcacgacgg cggacgcggc aatcgcctcg
     3901 taaactttgc gcacggtcgc gcattattat catgccgtag tgattgctgc ggatggcatt
     3961 ttcaggcacg ccgcagccgt tatcctggac ggtcagtttg acctgattat cgttttgcgc
     4021 caccgtcacc acgacctcac tcgcttgcga atgtttgagg gcgttactta atgcctcacg
     4081 ggcaatttgc aacaagtgga ttgcctgatg cgaaggcacc agacgaggcg gcaattgata
     4141 atccagcttc accgggaagc caaatttggc gctgtactct tcgcaactcg cctccagcgc
     4201 cggacgtaat ccaggctcgg tgagctgcaa gcggaatgtg gtgagcaatt cacgcaactg
     4261 cgcccaggat gcattcagtt cgttacggat ctgacttaac agttcgcggc tgctttctgg
     4321 cagcgcatcg ccctgcatct gtaaacaact cacctgcatc ttcatgcaag agagagattg
     4381 ggcaatagaa tcatgcagtt cgcgcgcaat ggtggcacgc tcttccatca cgatcaactg
     4441 ttgctgacgt tcctgatgcc gatccagcgc cagcgtggcg gtgagttgtt caaccagggt
     4501 atccaccagt tgttgttgat catggctaag atgacgcccc tgcggtaggg tcgccagcaa
     4561 aataccgtac tgcgtatgag agtcagccag ccgccacttc agggtcgtgc cgcgatcacc
     4621 aacgggtaat acgccgcgcg ggcagagctg gcagccttta tcatcacaag tcatatctgg
     4681 ctggcaggta aactcctgat gattctcttc atcatcagtg tcatacaccc gcagttcgat
     4741 atcacgtagc agggttaaat tctgtaagcc gttgagtaca ggtgacaggc gttcacacag
     4801 cggggcgcgg gaatgcaaac ggcggttagc ctgccataaa aaagagagga tctgattttt
     4861 atgctccagc ccggcggttt tctcctgaac ccgctgctca agtacggcat aactttcggc
     4921 cagttctgca gacatattgt tcaacgcagt tccaagcatc gccatttcgt tgcgcccgct
     4981 gatgtttgcg cgttgggtaa aatcgcgatg gctgacggca ctcgccattg ccagcagttg
     5041 ccgccacggt tgtagcagtc gcgcccgcaa ccagataata gtgaacacca gtaaaagtgc
     5101 cataaatacc gccattaccc gatggaccag taccactgtc tcaatgcgca tttccgtggt
     5161 gcggtcaaaa ccagatacca gctgatcaag cccggcaaca aactggctga catccgctga
     5221 caccgtttcg cggttttgtg cacgcatcag tgcagggatg agttcattac gccagtaatc
     5281 ttgtaaaccc tgtaattgcg ccagttgtcc gtctcgttct gctgctcgag tcaactcggc
     5341 gctaaatgcc gtttgttcca tctctttaat taagggcttg tctttctcgc ttaatggcac
     5401 tgctgccaac agacggtaac tttgcatgcg cagcgatccc gctttgttga tcgcatgggc
     5461 gctgccctga acgccttgca ccagccagcc agaaaccgcc atccctgcca gtccaatagc
     5521 agtagaaagc aacacaataa gcgcaacctg attaaccagg gtgagcggag agagacaacg
     5581 tttaagcatg taaacctctt ccttcaggct ttaaatgagc aataacctta atgaatgtga
     5641 cgatacattc tggaatggca gtattctcgg ctattggctg aagtataccc atacccggaa
     5701 agagttactc cttatttgcc gtgtggttag tagctttaca tcggtaaggg tagggatttt
     5761 acagcaccgt gaaaaatctc ataattttta tgaaatcact gtactcacta tgggtaatga
     5821 taaatatcaa tgatagataa agttatctta tcgtttgatt tacatcaaat tgcctttagc
     5881 tacagacact aaggtggcag acatcgaaac gagtatcaga ggtgtctatg agtcactcat
     5941 ccgcccccga aagggctact ggagctgtca ttacagattg gcgaccggaa gatcctgcgt
     6001 tctggcaaca acgcggtcaa cgtattgcca gccgcaacct gtggatttcc gttccctgtc
     6061 tgctgctggc gttttgcgta tggatgttgt tcagcgctgt tgcggtgaac ttaccgaaag
     6121 tcggttttaa ttttacgacc gatcagctat ttatgttgac tgcgttgcct tcggtttctg
     6181 gcgcgttatt acgtgttcca tactccttta tggttcctat cttcggtggt cgtcgctgga
     6241 cggcgttcag caccggtatt ctgattattc cttgtgtctg gctgggtttt gccgtgcagg
     6301 atacctccac gccttatagc gtcttcatca tcatctctct gctgtgcggc tttgctggcg
     6361 cgaacttcgc atccagtatg gcaaacatca gcttcttctt tccgaaacag aagcagggtg
     6421 gcgcgctggg cctgaatggt ggtctgggca acatgggcgt tagcgttatg cagttggttg
     6481 ctccgctggt ggtatcactg tcgattttcg cagtatttgg tagccagggc gtcaaacagc
     6541 cggatgggac tgagctgtat ctggcgaatg cgtcctggat atgggtgccg ttccttgcca
     6601 tcttcaccat tgcggcgtgg tttggcatga acgatcttgc tacctcgaaa gcctccatca
     6661 aggagcagtt gccggtactc aaacggggtc atctgtggat tatgagcctg ctgtatctgg
     6721 caaccttcgg ttccttcatc ggcttctccg cgggctttgc gatgctgtca aaaacgcagt
     6781 tcccggatgt tcagattctg caatacgctt tcttcgggcc gtttattggt gcgctggcgc
     6841 gttctgcagg tggtgcatta tctgaccgtc tgggcggaac tcgtgtcacg ctggtgaact
     6901 ttattctgat ggcgattttc agcggcctgc tgttcctgac cttaccgact gacgggcagg
     6961 gcggaagctt catggcgttc ttcgcggtct tcctggcgct gttcctgaca gctgggctgg
     7021 gtagtggttc cactttccag atgatttccg tgatcttccg taaactgaca atggatcgcg
     7081 tgaaagcaga agggggttct gacgaacgtg cgatgcgtga agcggcaacc gacacggcgg
     7141 cggcgttggg tttcatctct gcgattggcg cgattggtgg cttctttatc ccgaaagcgt
     7201 tcggtagctc gctggcatta acgggttcgc cagtcggcgc aatgaaagta tttttgattt
     7261 tctatatcgc ctgcgtagtg attacctggg cggtatatgg tcggcattct aaaaaataaa
     7321 tctta
//
LOCUS       DBFIFM010000210         7140 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.210, whole genome shotgun sequence.
ACCESSION   DBFIFM010000210 DBFIFM010000000
VERSION     DBFIFM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.210"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            71..574
                     /gene="dps"
                     /locus_tag="N7C74_003999"
                     /gene_synonym="pexB"
     CDS             71..574
                     /gene="dps"
                     /locus_tag="N7C74_003999"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HGU2433775.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            978..1724
                     /gene="glnH"
                     /locus_tag="N7C74_004000"
     CDS             978..1724
                     /gene="glnH"
                     /locus_tag="N7C74_004000"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HGU2433776.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            1862..2521
                     /gene="glnP"
                     /locus_tag="N7C74_004001"
     CDS             1862..2521
                     /gene="glnP"
                     /locus_tag="N7C74_004001"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HGU2433777.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2518..3240
                     /gene="glnQ"
                     /locus_tag="N7C74_004002"
     CDS             2518..3240
                     /gene="glnQ"
                     /locus_tag="N7C74_004002"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HGU2433778.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            3357..5582
                     /gene="ybiO"
                     /locus_tag="N7C74_004003"
     CDS             3357..5582
                     /gene="ybiO"
                     /locus_tag="N7C74_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HGU2433779.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEF
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQILSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLGSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(5579..6505)
                     /gene="rlmF"
                     /locus_tag="N7C74_004004"
     CDS             complement(5579..6505)
                     /gene="rlmF"
                     /locus_tag="N7C74_004004"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HGU2433780.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELKQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSECKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            6781..7041
                     /gene="mcbA"
                     /locus_tag="N7C74_004005"
     CDS             6781..7041
                     /gene="mcbA"
                     /locus_tag="N7C74_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HGU2433781.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
BASE COUNT         1705 a         1830 c         1817 g         1788 t
ORIGIN      
        1 ggaacacata gccggtgcta tacttagtct cgttaattac tgggacataa catcaagagg
       61 atatgaaatt atgagtaccg ctaaattagt taaatcaaaa gcgaccaatc tgctttatac
      121 ccgcaacgat gtctctgaca gcgagaaaaa agcaacagta gagttgctga atcgccaggt
      181 tatccagttt attgatcttt ctttgattac caaacaagcg cactggaaca tgcgcggcgc
      241 taacttcatt gccgtacatg aaatgctgga tggcttccgc actgcactga tcgatcatct
      301 ggataccatg gcagaacgtg cagtgcagct gggcggtgta gctctgggga ccactcaagt
      361 tatcaacagc aaaaccccgc tgaaaagtta cccgctggac atccacaacg ttcaggatca
      421 cctgaaagaa ctggctgacc gttacgcaat cgtcgctaat gacgtacgca aagcgattgg
      481 cgaagcgaaa gatgacgaca ccgcagatat cctgaccgcc gcgtctcgcg acctggataa
      541 attcctgtgg tttatcgagt ctaacatcga ataaatccat cgctgatggt gcagaatttt
      601 agtacccgat aaaagcggct tcctgacagg aggccgtttt gttttgcagc ccgcctcaac
      661 gcacttattt agtgcatcca tctgctatcc ccagctgatt aagtaaattt tttgtatcca
      721 catcatcaca caatcgttac ataaagattg ttttttcatc aggttttacg ctaaataatc
      781 actgtgttga gtgcacaatt ttagcgcacc agtttggtgc cccagaatgg tgcatcttca
      841 gggtgttgcc ctataaatcg tgcatcacgt ttttgccgca tctcgaaaaa tcaatgagtt
      901 gcaaaactgg cacgattttt tcatatatgt gaatgtcacg caggggatcg tcccgtggat
      961 agaaaaaagg aaatgctatg aagtctgtat taaaagtttc actggctgca ctgaccctgg
     1021 cttttgcggt ttcttctcat gccgcggata aaaaattagt tgtcgcgacg gataccgcct
     1081 tcgttccgtt tgaatttaaa cagggcgata aatatgtggg ctttgacgtt gatctgtggg
     1141 ctgccatcgc taaagagctg aagctggatt acgaactgaa gccgatggat ttcagtggga
     1201 tcattccggc actgcaaacc aaaaacgtcg atctggcgct ggcgggcatt accatcaccg
     1261 acgagcgtaa aaaagcgatc gatttctctg acggctacta caaaagcggc ctgttagtga
     1321 tggtgaaagc taacaataac gatgtgaaaa gcgtgaaaga tctcgacggg aaagtggttg
     1381 cggtgaagag cggcactggc tccgttgatt acgcgaaagc aaacatcaaa actaaagatc
     1441 tgcgtcagtt cccgaacatc gataacgcct atatggaact gggcaccaac cgcgcagacg
     1501 ccgttctgca cgatacgcca aacattctgt acttcatcaa aaccgccggt aacggtcagt
     1561 tcaaagcggt aggcgactct ctggaagcgc agcaatacgg tattgcgttc ccgaaaggta
     1621 gcgacgagct gcgtgacaaa gtcaacggcg cgttgaaaac cctgcgcgag aacggaactt
     1681 acaacgaaat ctacaaaaaa tggttcggta ctgaaccgaa ataataacgc tacacctgta
     1741 aagcgcactg gcagttccct ctcccctctg gggagaggat tagggtgagg gcgcaaaccc
     1801 gctccggggc cattaattac cctgaatttg attatttaca ccacggtaac aggaacaaca
     1861 tatgcagttt gactggagtg ctatctggcc tgccattccg cttctgattg aaggtgccaa
     1921 aatgaccctg tggatatcgg tcctcggtct ggctggcggc ctggtgatcg gattgctggc
     1981 aggttttgca cgcaccttcg gtggttggat agccaaccac gtcgcgctgg tttttattga
     2041 agtgatccgc ggcacaccta tcgtcgtcca ggtgatgttt atctattttg ccctgccgat
     2101 ggcgtttaat gacttacgca tcgacccatt tactgcggcg gtggtcacca tcatgatcaa
     2161 ctccggcgcg tacattgcgg aaatcacgcg tggtgcggtg ctgtctatcc acaaaggttt
     2221 tcgtgaagca ggactggcgc tcggtctttc acgttgggaa accattcgct acgtcatttt
     2281 accgctggca ctgcgtcgta tgctgccgcc gctgggtaac cagtggatca tcagcattaa
     2341 agacacctcg ctgtttattg tgatcggcgt ggcggaactg acccgtcagg ggcaagaaat
     2401 tattgccggt aacttccgcg cccttgagat ctggagcgcc gtggcggtgt tctatctgat
     2461 tattaccctg gtgctgagct ttattctgcg tcgtctggaa agaaggatga aaatcctgtg
     2521 attgaattta aaaacgtctc caagcacttt ggcccaaccc aggtgctgca caatatcgat
     2581 ttgaacattg cccagggcga agtcgtggtg attatcgggc cgtccggttc cggtaaatcg
     2641 accctgctgc gctgcatcaa caaactggaa gaaatcacct ccggcgatct gattgtcgat
     2701 ggcctgaagg ttaacgatcc gaaagttgac gagcgcctga ttcgccagga agcaggtatg
     2761 gtgttccagc agttttacct cttcccgcat ctgacggcgc tggaaaacgt catgtttggc
     2821 ccgctacgcg tgcgtggcgc gaacaaagaa gaggcggaaa aactggcacg tgagctgctg
     2881 gcgaaagtcg gtctggcaga acgtgcacat cactaccctt ccgaactttc tggtggtcaa
     2941 cagcagcgtg tggcgattgc ccgcgcgctg gcggtgaagc cgaaaatgat gctgtttgat
     3001 gaaccgactt ccgctcttga cccggaactg cgccatgaag tgctgaaggt tatgcaagat
     3061 ctggctgaag aagggatgac gatggtgatc gtgacccacg aaatcggttt tgccgagaaa
     3121 gtagcttcgc gcctgatctt tatcgacaaa ggccggattg cggaagatgg cgatccacaa
     3181 gtgttgatta aaaatccgcc gagccagcgc ttgcaggaat ttttacagca cgtctcttaa
     3241 cttctctgcc tccctttcac ccgaaaggga ggtacaccag attcctctca tttaaaatca
     3301 cccctccccc agcatctata cttatctttt tgctctgttt tctcactgga ggagtcatgc
     3361 ggtggatcct gttcatcctc ttctgcctgc tgggcgcacc tgcccacgcg gtatccatac
     3421 ccggcgttac aaccacaacg acaacggact caacgactga accggccccg gaaccggata
     3481 tcgaacaaaa aaaagcggct tatggcgcac tggcggatgt gctggataat gacacctcgc
     3541 gtaaagagtt gatcgaccag ttgcgcaccg tcgccgcgac gccacctgct gaaccggtcc
     3601 cgaagatcgt gccgccgacg ctggttgaag agcaaaccgt gctgcaaaag gtcaccgaat
     3661 tcagccgcca ttatggtgaa gccctttccg cccgcttcgg gcagctttat cgcaatatca
     3721 ccggctcccc gcataagccg tttaatccac aaaccttcag caatgcgctg acccattttt
     3781 caatgttagc ggttttagtg tttggttttt actggctgat tcgcctgtgc gcactgccgc
     3841 tgtatcgcaa aatgggtcag tgggcgcggc aaaaaaatcg tgagcgcagt aactggctgc
     3901 agcttccggc gatgattatc ggggcgttta ttatcgacct gctgttactg gcactgacat
     3961 tatttgtcgg ccagatatta agcgacaacc tgaatgcagg cagtcgcacc atcgctttcc
     4021 aacaaagttt gtttctcaac gcctttgccc tgattgaatt tttcaaagcc gtactacgcc
     4081 tgattttttg cccaaacgtg gcggaactgc gcccgttcac gattcaggac gagagcgccc
     4141 gttactggag tcgtcgcctg agctggttag gcagtttgat aggttatggc ctgattgtgg
     4201 ccgtgccgat tatctctaat caggtgaatg tacagatagg tgcgctggcg aacgtcatca
     4261 ttatgctgtg catgaccgtc tgggcgttgt acctgatctt tcgtaataaa aaagagatta
     4321 cccagcattt gctcaacttt gcggagcatt cgctggcctt tttcagcctg tttatccgcg
     4381 cctttgcgct ggtgtggcac tggctggcaa gcgcctattt tatcgtgctg tttttctttt
     4441 cgttgttcga tccgggcaac agcctgaaat ttatgatggg tgcaacagtg cgcagcctgg
     4501 cgattattgg tatcgcagca tttgtttccg gtatgttttc ccgctggctg gcaaaaacca
     4561 tcactctctc gccacatact cagcgtaact atccggagtt gcaaaaacgg ctgaatggct
     4621 ggctttcggc ggcgctgaaa acggcgcgta ttctgaccgt ctgcgtggcg gtaatgctgc
     4681 tgttgagcgc atggggattg ttcgatttct ggaactggct gcaaaacggc gcggggcaga
     4741 aaaccgtaga tatcctgatc cgtatcgcac tcattctttt cttctcggcg gttggctgga
     4801 cggtgctcgc cagtttgatc gaaaaccggc tggcttcgga tattcatggc cgcccgctac
     4861 ccagcgcccg cacgcgtacc ctgcttacgc tgtttcgtaa cgcgctggcg gtgattatca
     4921 gtaccatcac catcatgatt gtgttgtcgg aaatcggcgt caatatcgcg ccattgctgg
     4981 caggtgccgg ggcattaggt ctggctatct cgtttggttc gcaaacgctg gtgaaagata
     5041 ttatcaccgg ggtatttatt cagtttgaaa acggcatgaa cactggagat ttggtgacta
     5101 ttgggccgtt gaccggcact gtggaacgga tgtcgattcg ctccgtgggc gtacgccagg
     5161 ataccggggc gtaccacatc attccgtggt cttcgattac cacctttgcc aactttgtcc
     5221 gcggcattgg ttcggtagtg gcaaactacg atgttgatcg ccatgaagat gctgataaag
     5281 ccaatcaggc actgaaagat gcggtagcgg aattaatgga aaacgaagaa attcgtgggc
     5341 tgattattgg tgaaccgaac tttgccggga ttgttggctt aagcaatacc gcgtttacac
     5401 tgcgtgtttc gttcaccacg ctgccactca aacagtggac ggtacgcttt gccctcgaca
     5461 gccaggtgaa aaaacatttc gacctggcgg gcgttcgcgc gccggtgcag acttatcagg
     5521 tgctgcctgc tccgggcgcg accccggctg aaccgttacc gccgggggag ccaacgcttt
     5581 aacgctggcg attgacaaaa cggcggcgct gctcgtcgtt cataaaggtc caggcaataa
     5641 agcgactctg cttttgcccc tgggccatct cttttttaac caccttcacc gcgcccacgt
     5701 ccgtcagggc acgatacaac ggcggtaagt tttcaccacg agataccagt gaagtaaacc
     5761 acatcacctg cttcgcgaag cctttgctct cttcaatcat ctttttgata aaggtgacct
     5821 caccgccttc acaccacaac tcctgttgct ggccgccaaa gttcagtgca tcgtctttgt
     5881 tcagccccag gttacggcgt ttacactcac tacctgcccg cgccgcagcg gcggaatcgt
     5941 ggaatggcgg gttacacaag gtcgcgtcgt attgctcgtt tttatggatg atgccgttaa
     6001 aaatcgcccc actctctttt tgccgacgca gacgaatggc gcgattaagc cccggattag
     6061 cactgatgat cgcctgcgcg ctgcttaacg cctggctgct ggtttcacta ccggtaaaac
     6121 gccagccata ttcatgtacg ccaatcagcg gataaataca gttcgcacca acgccgatat
     6181 caagaatgct ggcattagct ggaattgttc cgcttgcttc agccagtaaa tcggcaaggt
     6241 gatgaatata atccgcccgg cccggtactg gcgggcagag aaaaccgtcc gggatatccc
     6301 agttcgctac ggcgtaaaaa tgggccagca acgccttatt gagcgccttc accgccagcg
     6361 gattggcaaa gtctacgctt tgctccccgg cgggtgtaag cgtgaggaat tgcttgagtt
     6421 caggattgac ctgacaaagc gtggcgagat cgtagcggct gtgatgacgg ttgcgcggat
     6481 gcaaccccgg tttctgggcg gacatagcat tctcctttaa gcagcggcgt aagatacccg
     6541 cataacgcga cagggtaaat atctggctgc gtttatcatt ccccaaaacc ttaacatggc
     6601 tatggtttat ggcaattcat cataaaaatc agcattatgc atgtcattga tttgtcttat
     6661 tttgtaaaaa tatatcgttc aaaaaataag cacatctctg tcaatttttt gcgtcatttg
     6721 catcgcgcag gcgacctaca cttatcaggc actacccgtt gtatttatga ggatgtcatt
     6781 atgaaaaagt gcctcacact actgattgcc accgtcctga gcggaatctc tctcacggct
     6841 tatgccgcgc aaccgatgag taacctggac agcggtcagc tacggcccgc cggtaccgtt
     6901 tcggcaactg gcgcatcaaa cctaagcgat ctggaggata aactggcaga aaaagcgcgc
     6961 gagcaaggcg cgaaaggtta tgtcattaat tccgcaggcg gaaatgacca gatgttcggt
     7021 actgcaacca tctacaaata accccaccaa cgtaactgca ttcgcctctg gatgcagttt
     7081 tcgcctgttt tgtaatcaaa aaacatcaaa tatgaacgca atgtaaataa atgtatttct
//
LOCUS       DBFIFM010000211         7117 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.211, whole genome shotgun sequence.
ACCESSION   DBFIFM010000211 DBFIFM010000000
VERSION     DBFIFM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.211"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..4508)
                     /locus_tag="N7C74_004006"
     CDS             complement(<1..4508)
                     /locus_tag="N7C74_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU2433782.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQRAMAASQMKRAANDKAIAQM
                     LASKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSKNVKINGKPAARAAVTLPPP
                     PPPGAIPEVPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLGNLAKLGKLGNLLGKSGKLLGAKLGALLGKTGNSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDVISLGATLNFAFDGESDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPADRMYMLGGMSDTASNRITFERDTQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARLDYHLFYEYDAADRIIRWSDNDQT
                     WSRFTYDAQGRCVTVTGAEGYYNATLDYGDGCTTVTDGKGIHRYYYDPDGNILREEAP
                     DGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADGADWQYCYDERGLL
                     SNITAPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTPQGLLSGIFRQDERRLGIEYD
                     HHNWPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSGLERAGTGAE
                     GFRYDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKDTLQLTEVIN
                     PQGESYLYILDNCGRVTEERDWGGVVCRYRYDADGLCTARVNGLEETILYSRDAAGRL
                     AEVITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRRAIEYHYPDE
                     HTVIRCILPPEDERDRHPDGSLLKTTYRYNAAGELTEVILPGDETLTFSRDEAGREVL
                     RHSNRGFACEQGWNAAGQPVSQRAGLFPAEATWGGLLPSLLREYRYDSAGNVSGVTSR
                     EDYGRETHREYRLDRNGQVTAVTASGTGLGYGEGDETYGYDSCGYLKAQSAGRHRISG
                     ETDQYAAGHRLKQAGNTQYDYDAAGRMVSRTKHRDGYRPETERFRWDSRDQLTGYRSA
                     QGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYSVRHLVFNGF
                     ELISQQFSRVRQPHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWRPGQTSLWGL
                     ALSLPADTDYPAPRGERDPEADPGLLYAGQWQDAESGLCYNRFRYYEPETGMYLVSDP
                     LGLQGGEQTYRYVPNPCGYIDPLGLAICQLARWTKWGSEQSNISDVLNSLGNRALKYA
                     NGDWIKSEA"
     gene            complement(4528..4989)
                     /locus_tag="N7C74_004007"
     CDS             complement(4528..4989)
                     /locus_tag="N7C74_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HGU2433783.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITRDPVSQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5017..6918)
                     /locus_tag="N7C74_004008"
     CDS             complement(5017..6918)
                     /locus_tag="N7C74_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HGU2433784.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MEGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGAGTALENHFAVIPADRTWRPQPRLKPLVDGPQSAVVTGPEGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGNGIQAYTAIKEIMLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1316 a         2177 c         1939 g         1685 t
ORIGIN      
        1 gcctctgatt ttatccaatc accattagca tatttaagtg ctctattccc taatgagttc
       61 aaaacatcag atatgttgct ttgctcactc ccccatttcg tccagcgggc taactgacat
      121 atagccagcc ccaaaggatc gatataccca caaggattcg gcacataccg ataagtctgc
      181 tcccctccct gcaaccccag cggatcactc accaggtaca tcccggtttc cggctcgtag
      241 taccggaacc ggttatagca cagccccgat tctgcatcct gccactgtcc cgcatacagc
      301 aggccggggt ccgcttccgg gtcccgctcc ccgcgcgggg ccgggtagtc tgtgtctgcg
      361 ggcaggctga gtgccagccc ccacaggctg gtctgccccg gccgccagac ggttttacct
      421 tcactgttaa agagcatcag cgggcggccc gtcaggtcgc tcaccgcatg attcgttcgc
      481 gtcacccact gcggggccac ggacggatgc ggctgtcgta cccggctgaa ctgctggctt
      541 atcagctcaa agccgttaaa caccaggtgc cttacgctgt acagtttatc atcgcggtat
      601 tcccggattt ccgcaatact gtcgccgtcc cacaggtacg taaaacggat tttcttccgg
      661 tcgcagcgtt tttccgtccg tctgccgctg gcgtcgtggc ggtattccca ctgctccccc
      721 tgtgcgctgc gatacccggt cagctggtcc cggctgtccc accggaaccg ctctgtttct
      781 gggcggtagc cgtcacggtg ttttgtgcgg ctgaccatcc ggcctgcggc gtcatagtca
      841 tactgtgtgt ttccggcctg tttcagccgg tggcctgcgg catactggtc agtctctccg
      901 cttatccggt gtctgcccgc agactgcgcc ttcaggtagc cgcagctgtc atagccataa
      961 gtctcgtcgc cttcgccata gcccagcccg gtgcctgagg ctgtcaccgc cgtgacctgg
     1021 ccgttccggt caagccggta ctcccggtgt gtttcccgtc cgtaatcttc ccggctggtg
     1081 acgcctgata cgttacccgc gctgtcgtaa cggtattccc gtagcagtga agggagcagt
     1141 ccgccccatg tggcttccgc cgggaaaagt ccggcgcgct ggctgacagg ctgaccggct
     1201 gcattccagc cctgttcaca ggcaaaaccc cggttactgt gccggagcac ttcacgtccc
     1261 gcctcatcac ggctgaacgt cagcgtctca tcccccggca ggataacctc cgtcagttct
     1321 ccggcggcgt tgtagcggta tgtggttttc agcagggatc cgtcggggtg tctgtcgcgt
     1381 tcatcttccg gtggcaggat acagcggatg acggtgtgtt cgtcggggta gtggtattca
     1441 atggcccgtc ggccctgagt ggtgacattc acccgcccgc gttcatcata gccggtgcgc
     1501 tgtgatgtgc cgtccgggct gaagataccc gtcagcctgc cggatttgtc atacgcatac
     1561 tgcgttttgc cttccggagt gatgacttct gccaggcggc ctgcggcatc ccggctgtag
     1621 aggatggttt cctccaggcc gttgaccctg gcggtacaca ggccatcagc gtcataacgg
     1681 taacgacaga ccacgccgcc ccagtcacgt tcttccgtca cccggccaca gttgtccaga
     1741 atataaagat aagactcgcc ctgcgggtta atgacttctg tcagttgcag cgtgtcttta
     1801 tcgtagcggt actgccagcg gtggccttca ccgtccgttc gcgccaccgg caaatcaaac
     1861 gggccgtact ccattgtcca gacaacgccg ttaccgtccg tgtacgccag caggttgccg
     1921 tggcggtcgt agcggaatcc ttccgcgccg gttcctgccc gctcaaggcc actcagttta
     1981 tggtgctgct gccactgcaa ccgcactgac tgaccgccgg ggccgcgcat cttcaccggc
     2041 aggtcgtgac cgctgtattc ggtgtggtgt tcgcggccca tcacgtcggt gagtgtttcc
     2101 ggccagttgt ggtggtcgta ctctatgccc agacgccgtt catcctggcg gaatatcccc
     2161 gacagcagcc cctgaggggt gtacgccagc cgggtctctt cgccctgtgg cgataccaga
     2221 ctcaccggca ggccgcgttc atcacactgc tgcgtccacg tctgcccggc aggggcggtg
     2281 atgttgctga gcaggccgcg ctcatcatag cagtactgcc aatccgcgcc gtctgccgcc
     2341 gtgtaacggc ttaactgacc gtgtgcggcg ttgtattcaa acttctccac ccgcccggca
     2401 ggggagtggc gggccagcag gtgatggaat tcatcccatt catacgtggt ggtgctgccg
     2461 tccggcgctt cttcccgcag aatattgccg tcaggatcat agtaataacg gtgaatgccc
     2521 ttgccgtccg tcacggtggt gcagccgtca ccatagtcca gcgtggcgtt gtaatagccc
     2581 tccgccccgg tgacggtcac gcaccggccc tgtgcatcgt aggtgaaacg gctccacgtc
     2641 tggtcgttat cggaccagcg gatgatccgg tccgcagcgt cgtactcata aaacaggtgg
     2701 tagtccagcc gcgcatcctg ttcgtaggtc gccagcgtct gtatgccgtt atccgtgcgg
     2761 tgaatggctt tcaggtagcc gctggcgtgg tacgtcagtt taagccggat cccgtcggtg
     2821 tgactgacac ccgtgatccg gtactgggtg tcgcgctcaa acgtgatgcg gttgctggcg
     2881 gtgtcgctca tcccgcccag catgtacatc cggtctgcgg ggctgtgtgc caggcggtca
     2941 tcgctggtat cgtcagtcac cgggtggcgc agacgcattc ccggaaaggc gtcataaaag
     3001 aagcggctgc tcagtgcatc cctgtcgaac agctccagat aatcatcacg gcggcgcaga
     3061 atgtactggg cgtgatacgg gttaaccgcc gtatcacttt caccgtcgaa ggcgaagttc
     3121 agcgtggctc ccaggctgat gacatccacc cggtttccct gttcacgcac ccaggcgtat
     3181 tcgctccagg agtcgctcca gcccacgccc gtcaggccat gcagcggcag tacagaactg
     3241 tggaaacggg tgaaggagag ggggagtgtc tggcccagcg taaagtcggt acgttcgtcg
     3301 cagtatgcgc cggttaccgg atcgacagga tctgttaccc atctgatgac tttattggca
     3361 atacttttta acgaattacc tgttttcccc agcaacgcgc cgagctttgc gcccagcagc
     3421 ttaccgcttt tccccagcag gtttcccagt ttgcccagtt tcgccagatt ccccagtttt
     3481 cccagcagtc ctgctggcgg cagaagatag ctggctatcg ttattcctcc caggatcatt
     3541 ctctgccatg gcgggaactc cggctggata tccagatagg tgacctgctc acccccaata
     3601 aagacgtccg gcgaaccttc cacgattgcc gcgctgcact ccagtttgtc gcccttgcgc
     3661 gcggcaggct gattgttgat aaagacgttg ctgctgccct gggctatcct cggtggtttg
     3721 ttctccttgt cgcagagcgc cgtgtcctca ccggcttccg ggaaatccac atcttttttg
     3781 atttcgccac gcccgcccac catggtggca aactccatta ataccggacc ggcgggaaac
     3841 agaagcgatg cgccggcgct gacgcgatcc ataaaaccgg cattgctgtc cgtcagggtg
     3901 ataacagcgt tcccccaggc cttcgccagt ggtactgctt tttccgctgc cgattcaagc
     3961 aggtcagtgg caatatcacc ccatgagggt tccccctgtg ggacttcagg tattgctcca
     4021 ggtgggggag gagggggaag ggtgacggct gcacgggctg ctggctttcc gttaattttt
     4081 acatttttag aaccggtatt gatggcgccg tccgtgtcac aaaagctgtt tatccatgat
     4141 tcaagctggt tctgtttctc ctccacataa tcactcgcct tatagctggc atacatcagc
     4201 gcaggcacca gaaccagcgc ataagggccg gtggcacagg cggccataat caggcaacct
     4261 tctgctatgg tcactatcgc ccccagcacg gcccctgcca gcgcccccaa aaaactcttg
     4321 tgctgaattt catcgcccag cctggcggcg ggggggccag acttttttga tgccaacatc
     4381 tgtgcaatgg ctttgtcgtt ggctgcccgt ttcatctggc tggcggccat cgcccgctgc
     4441 gacagcatcc gcattgccag cgtaccgcct gcggtcgctc cctgtggccc gcctggtcct
     4501 tcactcatgt tttatccctc gtttatgtca gcgcggctgg tccttttgga tgtcattctc
     4561 cggtggcaac ggggtgaagc tctttatcac ctccagcagc atcgtctttt gactgttctt
     4621 cagggataag gaggtgctga aagtgaaggt cagcagcgta tctccacgcc gctggaatac
     4681 ggctatctgg ctaatccgcc cctgattccc ttcatcttcc ggaccgttcc aggaataatc
     4741 cagcagccag gccggggcct ctgccagagt gatttcccgg caggcatgtt cggtgtaccc
     4801 atcaagtttg ccctggtaca gcgccagcgt ttcctggtac agctctggtg tcagggttcc
     4861 ctgtgaaacg gggtcgcggg taatgaccag gttgatgcca ctgttatccg tggataccag
     4921 gacctgcatc gactgatcct gccattgcga tggcagttga atatgacctt cattaaaagt
     4981 gaactgcata aaatactccc tgttaatctg agtgaatcag ttgatgaaaa cagaaccgcc
     5041 cacgatggtg atgccatcct tcgacagcct gattaccgtt gattccccga cctgtagctg
     5101 aatgccatcc ggcgtcagcg caattttgct ttcctccacg gccagcataa tttccttaat
     5161 tgccgtataa gcctgtatac cgttgccttc gccaccattg tttacagcgt caacatgcac
     5221 cgaaccggca atcgtgcctc tgtcctgaaa gacatcctca gataaagacc ctttcagtac
     5281 tccggtgatc ttgtggcctt tttccaccgt aagaaggcgg ttaccttcct ggatcgtgac
     5341 cgtctggtca ttttttacgg tttcgcgatg atcgtgtttt acggtggtgg tacggtcgtt
     5401 gagcacttcg gtatccatgt tcttctgcgc gtggatgtag acctgttctc tccctgtcgc
     5461 atcatcaaac ttcagctcat taaacccgct gcccatatac gttttggaac ggatggtcat
     5521 ctgcgtcttc gtccccggca ggctgccggg ggtgcggttt tcctggtggt aggtgcgccc
     5581 cataatgatc ggctggtccg gatcgccgtt gaggaagtcc actatcacct cctgacccac
     5641 acgcggtatc gccaggttgc caaaaccggt gcctgcccac gcctgtgcca cacggatcca
     5701 gcacgagctg tcctggtctg ccgggttata acggtcccag ttgaatttca cccgcactcg
     5761 gccatgttca tcacagaaga tttcctcacc ctccggcccc gtcacgacgg cgctctgcgg
     5821 accgtccacc agcggtttta gccgcggctg gggtcgccat gtccggtctg ccgggatcac
     5881 cgcaaaatgg ttctccagcg ccgtccctgc tccccgcctt cccggtaccg cctgcggctg
     5941 ctcgccgtgc agttcacttg ccaccacctg ccattcccgg ttcaggttcg cctgcggatg
     6001 ccccgtcagc acaattcgcc gtcccggcca tatctccggc gagcgactca ttccccgcgc
     6061 ggtttctgca ttgtttcgcc agccctccat ctgccagcgg gcaaagttct gcccgtgggc
     6121 gcccttgaaa cgtccggggt agtcatacac ctcatactgc gtgcgctggt aatcctggtg
     6181 ctggccttcc tgatcaaaac gtcctgccca gccggggcgt ttaaaggtgt agtctttggt
     6241 caccacggaa gaagggcgga tttgtgcgct gtagcgaaac tggctgatgc agagggtgct
     6301 cacctcggta cgggtgttcg ggttccaggg gatttcaaaa gattcgggca gatggcggac
     6361 cgtgtcgcac agcaccaggc tctggtcggt acttttgtaa gcatgctcct cataaaagaa
     6421 gatgccttcc tccgccgcca tccggcacag gaaatcgtaa tcagtctcac cgtactggac
     6481 acaaaactca cgggaaggat gcggttcact gaacagcgga ctccactcgg tcaccccgtt
     6541 ttcctgcaat atcgtgccga ggatgctttt gatgtcctcg ttctggaaga tacggaagtt
     6601 ctggcgcaga ccggcacgcc acagcggcgg gtgcaccttc atgctgtaca gcatctggtt
     6661 tttgtcgttc tcccccagtt caaaccaggt caccacacct ttcacccggc gctgtacatc
     6721 atcaccctgc catattgtca ggtaggccat tttgtccagc acctgcgcaa attcaaggga
     6781 gagaaactgc tggctgacca aggagagatc gagggaaaag agcgaagaga gtgactgggt
     6841 gagatgaaag gaaaccaccg caaaagcgtc cggtggcagg ccgtccactt ccagtgtgaa
     6901 acgtaatccg gttgacatag caacctccgg taaaatgaat aataatccgc accgtacaac
     6961 tgcggataat gagataagcg ggacgactta agacgttatt agggaaggaa caaaaaatgc
     7021 tatgattatg tgaggatttt gtttaaatat tactttacag aaacgggtgt ctgttcagtc
     7081 aaaaaaataa tcacaagctc aacttattgt ttcctga
//
LOCUS       DBFIFM010000212         7061 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.212, whole genome shotgun sequence.
ACCESSION   DBFIFM010000212 DBFIFM010000000
VERSION     DBFIFM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.212"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            229..645
                     /locus_tag="N7C74_004009"
     CDS             229..645
                     /locus_tag="N7C74_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HGU2433785.1"
                     /translation="MRKLTFELRSPIHQQNAIQAIQQILPDPTKPIVVTIQERNRSIR
                     QNARLHAMLSEISKKATYHGKARNIEFWKGLFVSGWQIATNQHPEIISGLEGELINIR
                     ESTATLSVKKISEIMDYIEAYCAMNSIHLSEWRNYD"
     gene            638..1240
                     /locus_tag="N7C74_004010"
     CDS             638..1240
                     /locus_tag="N7C74_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HGU2433786.1"
                     /translation="MIEVWVDIEGIPFYQVSNKGNFRSITREVTVTSTRQRPYKKIIN
                     GTSVKPFKCKSTGYLQIKVYGKKYSAHRIVAKAFCTGFCDGLVVNHKNGRRDDNRADN
                     LEWVSHSENSKHGYKQNGRIPISLGKFSGDHPASKAVISTDMKTGEEVYYEAAMDAVR
                     EGFDSSSISRCCNGESSYHKGRFWRFANEKMKARWGDRAA"
     gene            1237..1764
                     /locus_tag="N7C74_004011"
     CDS             1237..1764
                     /locus_tag="N7C74_004011"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HGU2433787.1"
                     /translation="MTIKSNTPAHNKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEVDDALNSEWVSHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            1761..1955
                     /locus_tag="N7C74_004012"
     CDS             1761..1955
                     /locus_tag="N7C74_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGU2433788.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARNTRGLTR"
     gene            2212..2886
                     /locus_tag="N7C74_004013"
     CDS             2212..2886
                     /locus_tag="N7C74_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HGU2433789.1"
                     /translation="MATKIAVETLSPITHNQIPVITTELLAHLYGTDVANIKMNHSRN
                     QTRFLEGKHYFKIVGDDLKNLRVTFSYLQISPKTRSLILWTERGAARHAKMLETDQAW
                     EVFEKLEDCYFSQCKKNTGKQEKKPNGLSAKETDSLVWLWDYANRSQALFRELYPALK
                     LIQSGYSGICHDYGYEFSYIIGRARGVLINHTRDIDIYEPDGPTNLLAWERLKNKELP
                     PSLHRY"
     gene            2961..3362
                     /locus_tag="N7C74_004014"
     CDS             2961..3362
                     /locus_tag="N7C74_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433790.1"
                     /translation="MLLIQPGFGLSIKKGHMFGEKESQRKMVSIRLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVKNHHAVRQAKRKANMTYMKAYKKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
     gene            3322..3531
                     /locus_tag="N7C74_004015"
     CDS             3322..3531
                     /locus_tag="N7C74_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HGU2433791.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCSDCCAELMSDPNSSMYEEEDDE"
     gene            3524..4246
                     /locus_tag="N7C74_004016"
     CDS             3524..4246
                     /locus_tag="N7C74_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HGU2433792.1"
                     /translation="MSELINGNAIKMTSIEIAELVGKRHDNVKRTIETLAKNGVIRLP
                     QIEVSERINNLGFNVQYEHYVFEGEQGKRDSIVVVAQLSPEFTARLVDRWRELEEAAV
                     NIPKTLPEALRLAADLAEQKMQLENQLAIAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGARRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            4246..4851
                     /locus_tag="N7C74_004017"
     CDS             4246..4851
                     /locus_tag="N7C74_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HGU2433793.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCCPEHGTQLALERRSKERE
                     KAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAATPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQE
                     AVDEIESNHNRHRWTVEECRAIKAKYQQKLKDLRNSRSEAA"
     gene            4848..5042
                     /locus_tag="N7C74_004018"
     CDS             4848..5042
                     /locus_tag="N7C74_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HGU2433794.1"
                     /translation="MTFTVKTIPDMLVEAYENQTEVARILNCSRNTVRKYTGDKEGKR
                     HAIVNGVLMVHRGWGKDTDA"
     gene            5035..5469
                     /locus_tag="N7C74_004019"
     CDS             5035..5469
                     /locus_tag="N7C74_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU2433795.1"
                     /translation="MRDIRQVLERWGAWAANNYEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            5748..5873
                     /locus_tag="N7C74_004020"
                     /pseudo
     CDS             5748..5873
                     /locus_tag="N7C74_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            5914..5989
                     /locus_tag="N7C74_004021"
     tRNA            5914..5989
                     /locus_tag="N7C74_004021"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5947..5949,aa:Ile,seq:cat)
     gene            5999..6075
                     /locus_tag="N7C74_004022"
     tRNA            5999..6075
                     /locus_tag="N7C74_004022"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6033..6035,aa:Arg,seq:tcg)
     gene            6089..6165
                     /locus_tag="N7C74_004023"
     tRNA            6089..6165
                     /locus_tag="N7C74_004023"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6123..6125,aa:Arg,seq:tct)
     gene            complement(6294..6437)
                     /locus_tag="N7C74_004024"
                     /pseudo
     CDS             complement(6294..6437)
                     /locus_tag="N7C74_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6677..>7061
                     /locus_tag="N7C74_004025"
     CDS             6677..>7061
                     /locus_tag="N7C74_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU2433796.1"
                     /translation="MTFKHYDVVRAASPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVSTPVVVKLTGGEGAVISATRDPEYYFIVVLAGQSNSMAYGEGLPLP
                     ETYDRPDPRIKQLARRSTVTPGGVAC"
BASE COUNT         2069 a         1538 c         1816 g         1638 t
ORIGIN      
        1 actcaataat gagtttcagc gcaacagaat tatccggagt ctggatttaa agtgaaggag
       61 tgagcatgag cgagcaaata ttcagagaga ttaagccacg gttttatcgc aaggtaaggg
      121 tggtttatca ggacgaaaac aagacatgtg catacgccat tcataatggt cggtggtcag
      181 tgttcgacac caaaaacttc gagaagaact tcgagaggat taagggtgat gaggaaacta
      241 acatttgaac taagaagccc catccatcag cagaacgcca ttcaagctat ccagcaaatt
      301 cttccagacc caaccaaacc aatcgtagta accattcagg aacgcaaccg cagcattcgg
      361 caaaatgcac gccttcacgc gatgctatct gaaataagta agaaggctac atatcacgga
      421 aaagcaagaa atattgagtt ttggaagggg ttattcgttt ctggttggca gattgcaacc
      481 aaccagcacc ctgagattat atcagggtta gaaggtgagc taataaacat cagagagagt
      541 acggcgactc tatctgtaaa aaaaatatcc gaaataatgg actacataga agcatattgt
      601 gccatgaact caattcatct tagcgaatgg aggaattatg attgaggttt gggtagatat
      661 cgaagggatt ccattttatc aggttagcaa taaaggaaat ttcaggtcta ttacgaggga
      721 agttacagta acatcaacca gacagaggcc atataagaaa ataattaatg gcactagtgt
      781 aaaaccattc aagtgcaagt cgacaggata tcttcaaata aaggtatacg gtaagaaata
      841 cagcgcccac aggatagttg cgaaagcatt ctgtacaggg ttctgtgatg gcttggtagt
      901 taatcacaaa aatgggcgaa gagatgacaa tagggctgat aaccttgaat gggtatcaca
      961 ttctgaaaac tcaaaacacg gatataaaca aaatggaaga atacctatat cgctaggtaa
     1021 atttagtggt gaccatcctg ccagtaaagc tgttatttct actgacatga aaactgggga
     1081 ggaggtttat tatgaagcag ctatggatgc tgtcagagaa ggatttgata gttcgtcaat
     1141 tagtcgttgc tgtaatggcg aaagctcata tcacaaagga agattctggc gatttgcaaa
     1201 tgaaaaaatg aaagcgcgat ggggagatcg ggctgcatga ctatcaaatc aaatacgcca
     1261 gcacacaaca aggactgctg gcaaacgccg ctttggcttt ttgatgcact ggatattgag
     1321 tttggattct ggctggattc agctgcgagc gacaaaaatg ctctgtgcgc tcactggcta
     1381 actgaggtcg acgacgcgct caattctgag tgggtaagcc acggtgcaat ctggaataac
     1441 ccaccgtaca gcaatatcag gccgtgggtg gaaaaagccg ctgagcagtg catacaacag
     1501 cgacagacgg tagttatgct tgtgccagag gatatgtcag tcggatggtt cagcaaggct
     1561 ctggagagtg ttgacgaagt tcgcattatc actgatggac ggattaattt tatcgaacca
     1621 tcgacggggc tggagaagaa gggaaacagt aaaggctcca tgctgctgat ttggcgaccg
     1681 ttcatcagtc ctcgacggat gtttactacc gtatccaaag cggcattgat ggcgatcggg
     1741 cagggcgtca ggagggcggc atgaggcgac agcgacgaag tatcaccgac atcatctgcg
     1801 aaaactgcaa ataccttcca acgaaacgct ccagaaataa acgcaagcca atcccaaaag
     1861 aatctgacgt aaaaaccttc aattacacgg ctcacctgtg ggatatccgg tggctaagac
     1921 atcgtgcgag gaatacaagg ggattgacgc gatgatttat ccggggctat attcctcaca
     1981 cgccagcaaa atctggcgtc gggattggcg tcccggatga aaaaggcgac aacagacgcg
     2041 ccagcgtctt ttttattgtc gtttgcacag tcacatctca atggtgggct gtgtgggggc
     2101 ggagcaatcc gcgccggttc ctttttcccg gttacgccaa ccctgcacag ttcaccacca
     2161 agcgattggc gtcgcaggtg gtgatgattc acaaagaaaa aggatcatct tatggccacc
     2221 aaaatcgcag ttgaaactct ttccccgatt acccacaacc agatccctgt cataaccacc
     2281 gagctattgg cgcatcttta tggaacggat gttgccaaca taaaaatgaa tcattcacgt
     2341 aatcaaactc gttttctgga agggaagcat tatttcaaaa tcgttggcga tgatctgaaa
     2401 aatttgcgag taacttttag ttacctgcaa atttccccca aaacccgctc cctcatcctc
     2461 tggactgaac gcggcgctgc ccgtcacgcc aaaatgctcg aaaccgatca ggcgtgggaa
     2521 gtgttcgaaa aactggaaga ctgctatttc agccagtgca agaaaaatac tggcaaacaa
     2581 gagaagaagc ccaacgggct ttccgcaaaa gaaacagaca gccttgtttg gctgtgggat
     2641 tatgccaacc gctcacaggc attgttccgt gagttgtatc ccgcattaaa actgattcag
     2701 tctggctatt ccggcatatg ccacgactac ggctatgagt tctcgtatat catcgggagg
     2761 gcgaggggcg ttttaattaa tcacacgcgg gatatagata tttatgagcc tgacgggccg
     2821 acgaaccttc tggcatggga aaggcttaag aacaaagagt tgccgccttc actgcatcgc
     2881 tactgacaat tgacaactta acaaacccag cttcggctgg gttttttatt gctgaatttt
     2941 caatgtgaga ggacatgaca atgcttttaa ttcaacctgg atttggcctt agcatcaaaa
     3001 aagggcacat gtttggcgag aaagagtctc aacgaaaaat ggtgtctatc cggttgccat
     3061 ttatcagtat ttattggcta aacagggagg caacaaatta ttggtatacc tgcgccagag
     3121 cagcatttaa cgaccctgac tggtttgtga aaaaccacca cgcagttcgt caggcaaaga
     3181 gaaaggccaa catgacatac atgaaggcgt ataaaaaagc atggaaagaa caccgcgacc
     3241 gataccagca agacatggaa aagcttgaat cagaaaacat ggaattaaga cgaaagctcg
     3301 gtgaagcaaa acgagacatt gatgcttaca agcgactttt taatggtgaa agccatgctt
     3361 agtccatccc aatcccttca ataccagaaa gaaagcgtcg agcgggcttt aacgtgcgct
     3421 aactgcggtc agaagctgca tgtgctggaa gttcacgtgt gctccgattg ctgcgcagaa
     3481 ctgatgagcg atccgaatag ctcaatgtac gaggaagaag acgatgagtg agttaataaa
     3541 tggcaatgcc atcaaaatga caagcattga aatcgctgag ttggtgggta agcgtcatga
     3601 caatgtgaaa cgtaccatcg aaacgctggc taaaaatggt gttatccggc ttcctcaaat
     3661 tgaggtttcc gaaagaatca ataacttagg gttcaatgtt cagtacgagc attacgtctt
     3721 cgaaggcgaa caaggaaagc gagacagtat tgtcgttgta gcccagctgt cgccggaatt
     3781 caccgctcgc cttgttgacc gctggcgaga gcttgaagaa gctgcggtta atatccccaa
     3841 aacgctacca gaagcgttgc gccttgctgc tgatcttgct gagcagaaaa tgcaactgga
     3901 aaaccagctc gcaattgccg cacctaaagt tgagtttgcc gatcgcgttg gcgaggccag
     3961 cggaattttg attggaaact ttgcaaaggt tgttggtatt ggtccaaaca aactgtttgc
     4021 gtggatgcgc gatcacaaaa tccttattgc ttcaggtgcc cggcgcaatg tgccaatgca
     4081 ggaatatatg gatcgcggct atttcacagt gaaagaaaca gcggtcaata caaatcacgg
     4141 aatacagata tcgttcacca caaaaatcac cgggcgtggt caacagtggc tgacaagaaa
     4201 gctgctagat aacggaatgc ttaaagtaac aggggaggct gcttaatggc taaaccagcg
     4261 cgaaggaaat gcaaaatctg taaggaatgg tttcacccgg cattctcaaa tcagtggtgg
     4321 tgctgcccgg aacacggaac tcaattagca ctcgaacgac gaagtaaaga acgcgaaaaa
     4381 gcggaaaaag cagcagagaa gaaacgacga cgagaggagc agaaacagaa agataaactg
     4441 aagattcgaa aactcgcctt aaagccccgc agttactgga ttaaacaagc ccaacaagcc
     4501 gtaaacgcct tcatcagaga aagagaccgc gacttaccat gtatctcgtg cggaacgctc
     4561 acgtctgctc agtgggatgc cggacattac cggacaactg ctgcgacacc tcaactccga
     4621 tttgatgaac gcaatattca caagcaatgc gtggtgtgca accagcacaa aagcggaaat
     4681 ctcgttccgt atcgcgtcga actgattaac cgcatcgggc aggaagcagt agacgaaatc
     4741 gaatcgaacc ataaccgcca tcgctggact gtcgaagaat gcagggcgat caaggcgaag
     4801 tatcagcaga aacttaaaga cctgcgaaac agcagaagtg aggccgcatg acgttcacag
     4861 taaaaaccat tcctgacatg ctcgttgagg catatgaaaa tcagaccgag gtagccagaa
     4921 tactgaactg tagtcgcaac acggtcagaa aatacactgg cgataaagaa gggaaaagac
     4981 acgctatcgt caacggtgtt cttatggttc accgcggatg gggtaaagat actgatgcgt
     5041 gatatccggc aggttcttga gcgctggggg gcatgggcgg caaataacta tgaggatgtt
     5101 acatggtcgc ccattgctgc cggatttaag ggactgatcc ccgaaaaagt aaaatcacgt
     5161 ccacagtgct gtgacgatga tgcgatggtg atatgcgggt gcatagcccg cctttaccgg
     5221 aacaatcgcg atctgcatga cttgctggtt gattattacg tgttggggga gacgttcatg
     5281 gcgttggcac ggaaacatgg gtgctctgac acctgtatag gtaaacgcct tcacaaagcg
     5341 gaggggattg ttgaaggcat gctgatgatg ctgggagtga ggcttgagat ggatcggtat
     5401 gttgagcgtg aattgccggg agggagaacc tctgtatttt atcagcgaaa aaatagttta
     5461 cgatcgtaaa aatctgcata tcatgataag agtggttaca ttgccacgct gcttattaac
     5521 ccgccgatgc gcgggttttt ttgtacccag aatcctgtga gctatacgga aagtacacag
     5581 aaaggaaggt gcgaccacaa ttaataacaa aatcttaaaa attgcacatg gcactattag
     5641 ttttctaaat attgtgtatt ttttgtattg caggatgacc ctgtaacgaa gtttgcgtaa
     5701 cagcattttg ctctacgagt ttgccagcct cccccagtgg ctggcttttt tatgtccgta
     5761 gcgtcaaagc agcaatgtcg ctggggcgtc gtgcaattgg cgttgagctg gagactgaac
     5821 gttttgagca gacggtcagg gaagttcagg atttagtcag tcagaacgga tgatattgca
     5881 ggattagtta cgtaccgtta ttatcctgcg cccggccctt tagctcagtg gtgagagcga
     5941 gcgactcata atcgccaggt cgctggttca aatccagcaa gggccaccat atcacatacc
     6001 gccattagct catcaggaca gagcgccagc cttcgaagct ggttgcgcgg ggttcgggtc
     6061 cccgatggcg gtccattatc ggtattcagc gttgttagct cagccggaca gagcaattgc
     6121 cttctaagca atcggtcact ggtccgaatc cagtacaacg cgccacacca cacttatctg
     6181 ccctgactct cttttgcggg ctttttatta caggaaagac accggacagt gaaatgttaa
     6241 atgcctcaca attcaggcag ttgaatgttg cctgacatgc tgagcgtttg ttaaaaaaat
     6301 ttcgcatggt gaatccccct gtgcggaggg gcaatcagca accaggtata tgtgataatc
     6361 gcggattcag gtgctgatac tgaattcacc gggaggcacc cggcaccatg caagaaaaag
     6421 aatgtgcatg caaacatgcc cctctccgga ggggcttttt tatgggtaaa aaatgcccga
     6481 atgggttcgg gcaatagcat gagatactga tattgttgtg ttgttatcgt gtggatttta
     6541 accagggttt atcaggctgc gcaactgcgt ggcctttttt catttcttgg gctgtagtcc
     6601 ccgtgtgtca ttcaggcttc cggactacag cccactccat atctgattta atacactatc
     6661 ccggccggga ggaataatga catttaaaca ttatgatgtt gtcagggcgg cgtcgccgtc
     6721 agaccttgct gatgcacttg cgcaaaaaat tcgtgaagga tggcaaccat acggtgggcc
     6781 gttttcttcg tatacggatg atggcgcagc acttattcag gcgattgtcg cagaaggtga
     6841 tgtgagcaca cctgttgtgg tgaagctgac aggtggagaa ggtgcagtaa tcagcgccac
     6901 cagagacccg gagtattact ttattgtggt tctggcgggg cagtcaaaca gcatggcata
     6961 tggtgaaggc cttccgctgc cggagacata tgaccgtccg gacccgcgta ttaagcagct
     7021 ggcgcgccgc agtacggtga caccgggcgg tgtcgcctgt a
//
LOCUS       DBFIFM010000213         6981 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.213, whole genome shotgun sequence.
ACCESSION   DBFIFM010000213 DBFIFM010000000
VERSION     DBFIFM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.213"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            165..683
                     /locus_tag="N7C74_004026"
     CDS             165..683
                     /locus_tag="N7C74_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HGU2433797.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            893..3034
                     /locus_tag="N7C74_004027"
     CDS             893..3034
                     /locus_tag="N7C74_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HGU2433798.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLALSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQKLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIASILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVLYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQILNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3110..>6981
                     /locus_tag="N7C74_004028"
     CDS             3110..>6981
                     /locus_tag="N7C74_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element core protein"
                     /protein_id="HGU2433799.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPVLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDNGGRSIHFEPLFPGEISYSRSESFWLARGGVAEQHSSQPLSALWQ
                     VLPEDVRLSPHMYLATNSLQGPWWILNWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPAGPRSA
                     SSSLVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDELPAAPLARYTYTASGELRAV
                     YDRSGTQVRGFAYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTELVNPEGLDYRFEYG
                     QDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRR
                     TEYSLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKGRLAAET
                     SRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLTFTDCSGYTTRYEYDRY
                     GQQIAVHREEGISTYSSYNPRGQLVSQKDAQGRETRYEYSAAGDLTAIVAPDGSRSEI
                     QYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGR
                     TQRYHYDLTGKLTQSEDEGLVTLWHYDASDRITHRTVNGDPAEQWQYDEHGWLTTLSH
                     TSEGHRVSVHYGYDDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLATRQEPDGL
                     PPVEWLTYGSGYLAGMKLGGTPLVEYMRDRLHRETARSFGGEAYELATAWNTSGQLRS
                     RHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPYAT
                     DPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYIYRYDEYGRLAEKTDRIPEGVIRMHD
                     ERTHHYHYDSQHRLVFHTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTGWMSLS
                     RKPEETWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAKARHRSLAEV
                     LQEDTGVTLPAELAVMLGRLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAEYIPE
                     RKLHLYHCDHRGLPQALISPEGETAWQGEYDEWGNLLGETSAQHLQQPYRLPGQQYDE
                     ESGLYYNRHRYYDPLQGRYITQDPIDIKGGWNLYSYALNPVSWIDPLGLTQCDSEGCN
                     NDILFTGGSGPDNKILNELGPRDGIDGLG"
BASE COUNT         1613 a         1836 c         2269 g         1263 t
ORIGIN      
        1 attttaaaaa tagtttttac gacaaccgta aattctagct cggtttgtat gtaaaaaata
       61 ctgaaaaatg atgcggttcc gtgaagtgag ctgcatctgc ctgtctggga ttaacggcag
      121 gtcattgtaa gtagtaatta accacgttaa caaggagttt acaaatgcca acaccgtgtt
      181 atatctccat cactggtcag acccagggaa atattactgc aggtgcattt accgctgatt
      241 ctgtcggcaa catctatgtg caggggcacg aagacgagat gctggtgcag gaattcctgc
      301 ataacgttac tgtcccgacc gatcctcagt ccggtcagcc tgcgggtcag cgtgctcaca
      361 agcctttcat ttttaccgtg gcgctgaaca aagcggttcc gctgatgtac aacgcactgg
      421 cctccggcga aatgctgcca accactgaac ttcactggtg gcgtacctct gtggaaggca
      481 aacaggagca ttatttcact acccgcctga ctgattcgac gattgtcgat atgaaactgc
      541 atatgccgca ttgccaggac ccggcgaaac gcgagttcac ccagttgctc gaagtttcac
      601 tggcctaccg caaaattgaa tgggaacacg tcaaatctgg cacctccggt gccgacgact
      661 ggcgcgcacc gctggaagca taagttaagc caacagcatc cggctatcag gccggatgct
      721 gtttttgtat tacgccgtag aaagaaaggt tgtcagtaag gggttacgtt cacgggagat
      781 ggcggctgcc ggaaacttcg gcggtagcgt gcctgaacgc aagcgtagca ctgtactgtc
      841 acagccgctt taacagcgaa ctgtctgttt ttccttaacc ccggaggttg ctatgtcaac
      901 cggattacgt ttcacgctgg aagtggacgg cctgccaccg gatgcttttg cggtggtctc
      961 ctttcatctg aaccagtcac tctcttcgct tttttccctc gctctctctc tggtcagcca
     1021 gcagtttctc tcccttgaat tccagcagat cctcgacaaa atggcctacc tgacgatatg
     1081 gcagggcgat gacgtacagc gccgggtgaa aggtgtggtg acctggtttg aactggggga
     1141 gaacgacaaa aaccagaagc tgtacagcat gaaggtatgc ccgccgctgt ggcgcacagg
     1201 gctgcgccag aacttccgta tcttccagaa tgaggacatc gcaagcatcc tcggcacgat
     1261 attgcaggaa aacggggtga ccgagtggag cccgctgttc agcgagccac atccttcccg
     1321 tgagttttgt gtccagtacg gtgagactga ttacgatttc ctgtgccgga tggcggcgga
     1381 ggaaggcatc ttcttttatg aggagcacgc gcaaaaaagt accgaccaga gcctggtcct
     1441 gtgcgatacc gtgctttatc tgccggagtc ctttgagatc ccctggaatc cgaacacccg
     1501 taccgaggta agcaccctct gcatcagcca gtttcgctac agcgcacaaa tccgcccttc
     1561 ttccgtggtg accaaagact acacctttaa acgccccggc tgggccggac gttttgacca
     1621 ggaaggccag caccaggatt accagcgcac acagtatgaa gtgtatgact atcccggacg
     1681 tttcaagggc gcccacgggc agaactttgc ccgctggcag atggacggct ggcgaaacaa
     1741 cgcagaagtg gcgcgcggaa caagccgttc gccggagata tggccgggac ggcgaattgt
     1801 gctgacgggg catccgcagg cgaacctgaa ccgggaatgg caggtggtgg caagtgaact
     1861 gcacggtgag cagccgcagg cggtgccggg acgcaggggt tcaggcacca cgctggataa
     1921 ccattttgcg gtaataccgg cagacagaac atggcgacca cagccgttgc tgaaaccgct
     1981 ggtggacggc ccgcagagcg ccgtcgtgac gggaccggca ggcgaggaaa tcttctgtga
     2041 tgaacatggc cgcgtgcggg tgaaatttaa ctgggaccgc tataacccgt caaaccagga
     2101 cagttcatgc tggatccgtg tggcacaggc gtgggcaggc accgggtttg gtaacctggc
     2161 gataccgcgt gtgggtcagg aggtcattgt ggacttcctc aacggcgatc cggaccagcc
     2221 gatcattatg gggcgtacct accaccagga aaaccgcaca cccggtagcc tgccggggac
     2281 gaagacgcag atgaccattc gttcgaaaac ctataagggc agcgggttta atgaactgaa
     2341 gtttgacgat gcgacaggga aagaacaggt ctacatccac gcgcagaaga acatgaacac
     2401 cgaggtgctg aataaccgca ccacggatgt gataaacaac catgccgaaa aaataggtaa
     2461 caaccaggcg atcaccgtta ccaataacca gatcctgaac attggcgtta atcagataca
     2521 gacggttggt gtcaaccagg tggaaacggt aggcagtaac cagattatca aagtgggatc
     2581 aaaccaggtt gaaaaggtgg ggatcattcg tgcgctgacg gtgggtgtgg cgtatcagac
     2641 gacggtaggc ggcattatga atacctcggt ggcgttgttg cagtcctcac aggtagggct
     2701 gcataaatca ctgatggtgg gaatgggcta cagcgtcaat gtagggaata acgtcacctt
     2761 ctcggtgggc aagacgatga aggaaaacac cggacaaaca gcagtttatt ctgccggtga
     2821 gcatcttgaa ctctgctgtg gtaaggcaag gctggtgctg acgaaggacg gaagcatatt
     2881 tcttaatggt acgcacattc atctggaagg ggagtcggat gtgaacggtg atgcgccggt
     2941 gattaactgg aactgtggtg ccacacaacc tgtaccggat gcgcctgtgc cgaaagattt
     3001 accccccgga atgccggata tgcggcaatt ttgagtcagg tgcaaaaaat caaaacggaa
     3061 ctggtgcagt gaccagataa accatattaa aagcagaagg aattgattca tgggaggaaa
     3121 accggcggcg cggcagggtg acatgacccg caagggactg gatattgtgc agggttcagc
     3181 aggggtgctg ataggtgcgc cgacgggcgt ggcctgctcg gtgtgtccgg gagggattac
     3241 ctatgctaac ccggtgaacc cggtgctggg tgcgaaggtg ctgccgggcg agacggacct
     3301 tgcgctgccc ggcccgctgc cgtttattct ttcccgcgcc tacagcagct accggaccag
     3361 aacacccgcg ccggtggggg tgtttggtcc cggctggaaa gcgcccttcg atatccgctt
     3421 acagatacgc gatgaaggcc tgatactcaa cgacaacggc ggcaggagca ttcactttga
     3481 gccgctgttt cccggcgaga taagctacag ccgcagcgag tcgttctggc tggcgcgggg
     3541 cggggtggcg gagcagcaca gttcgcagcc gctaagcgcg ctctggcagg tgctgccgga
     3601 agatgttcgc ctgagtccgc atatgtacct ggcgacaaac agccttcagg ggccgtggtg
     3661 gatactcaac tggccggagc gggtgccggg ggcggacgag gtgctgccgc cggagccgcc
     3721 cgcataccgg gtgctgacgg gcgtggtgga tggcttcggg cggacgctgg cctttcaccg
     3781 ggcggcggag ggtgatgtgg cgggcgcggt gacgggggtg acggacggcg cgggccgccg
     3841 ttttcacctg gtgctgacca cacaggcaca gcgggcggaa gtattccgta aacagcgcgc
     3901 cacgtcttta tcttcccctg ccggtccccg ttctgcttcc tcttctctgg ttttccccga
     3961 cacgctgccc gccggtacag aatacggtgc cgataacggt atccggctgg aggcggtatg
     4021 gctgacacac gacccggcat acccggatga actgcccgcc gcgccgctgg cgcgctacac
     4081 gtacacggcc agcggagaac tgcgggcggt gtatgaccgc agcgggacgc aggtgcgcgg
     4141 gtttgcttat gatgcggagc acgccgggcg gatggtggcg caccattatg cgggtaggcc
     4201 ggagagccgc taccggtatg atgataccgg ccgggtgacg gagctggtca acccggaggg
     4261 gctggactac cgctttgagt acgggcagga ccgtgtgacc atcacggaca gcctgaaccg
     4321 gcgggaggtg ctgtacacgg aaggcgaggg tggcctgaaa cgtgttgtga agaaggaaca
     4381 tgcggacggg agcatcaccc gcagcgagta tgatgaggcg gggaggctga aggcacagac
     4441 ggatgcggcg ggacggcgga cggagtacag cctgcatatg gcgtcgggtg cggtgacagc
     4501 ggtgacgggg ccggacggca ggacggtgcg gtatggctat aacagccagc ggcaggtgac
     4561 gtcagtgacg tacccggacg ggctgcgcag cagccgggag tatgatgaga agggaaggct
     4621 ggcggcggag acctcgcgca gcggagagac gacgcggtac agctatgatg acccggcgag
     4681 tgagctgccg acagggatac aggacgcgac gggcagtaca aaacagatgg catggagccg
     4741 ttacggtcag ctgctgacct ttacggactg ctcggggtac acgacgcggt atgagtatga
     4801 ccggtacggt caacaaatcg ccgttcaccg ggaagaaggc atcagcactt acagcagtta
     4861 taacccgcgt ggccaactgg tcagtcagaa ggatgcgcag gggcgtgaaa cccgttatga
     4921 gtacagcgcc gcaggcgacc tcaccgctat cgttgccccg gacggcagcc gcagtgagat
     4981 acagtatgat gcgtggggaa aggccgtcag caccacgcag ggcggtctga cgcgcagcat
     5041 ggggtatgac gctgccgggc gcatcaccgt gctgaccaac gagaacggca gccagtccac
     5101 gttccggtat gacccggtgg acaggctgac tgaacagcgc ggttttgacg gccggacgca
     5161 acgttaccac tatgacctga ccggaaaact cacgcagagt gaagacgagg ggcttgtcac
     5221 cctctggcac tacgatgcgt cggaccgcat cacgcaccgg acggtgaacg gcgacccggc
     5281 agagcagtgg cagtatgatg agcacgggtg gctaaccacc ctcagccata ccagtgaagg
     5341 ccaccgggtg tcggtccact acggctatga cgataaaggc cgcctgacgg gtgaacggca
     5401 gacggtggag aacccggaga cgggggagat gctgtgggag catgagacgg ggcacgcgta
     5461 cagcgaacag gggctggcga cccgtcagga gccggacggt ctgccgccgg tagagtggct
     5521 gacgtatggc agcggttatc ttgcggggat gaagctgggc ggaacgccac tggtcgagta
     5581 catgcgggac cggctgcacc gtgagacggc ccgcagcttc ggcggggagg catatgaact
     5641 tgccaccgcc tggaatacca gcggccagct ccggagcagg cacctgaacc ttccgcagct
     5701 tgaccgtgac tacgactgga acgacaacgg acagctaatc cgcatcagcg gcccgcagga
     5761 gagccgggag taccgttaca gtgacacggg aaggctgacg ggcgtccaca ccacggcagc
     5821 gaacctggat atcgatatcc cgtatgcaac ggacccggca ggaaaccggc tgccggaccc
     5881 ggaactgcat ccggacagca cgctcacggc gtggccggat aaccgcatcg cggaagatgc
     5941 gcactatatc tatcgctacg atgaatacgg caggctggcg gagaagacgg accgtatccc
     6001 ggaaggggtt atccggatgc acgacgagcg cacccaccac tatcactacg acagccagca
     6061 ccgcctggtg ttccacacgc ggatacagca cggcgaacca caggtggaga gccggtacct
     6121 ctatgacccg ctgggccgcc ggacgggaaa acgggtgtgg cggcgggagc gtgacctgac
     6181 ggggtggatg tcgctgtcgc gtaaaccgga ggagacctgg tacgggtggg acggtgacag
     6241 gctgaccact gtacagaccc aacagacaag aatccagacg gtataccagc cgggaagctt
     6301 cacgccgctc ctgagaatcg aaacagagaa tggtgaacag gcgaaggcgc ggcaccgtag
     6361 cctggcggag gtgttgcagg aggacacggg tgtgacgcta ccggcggagc tggcggtgat
     6421 gctggggagg ctggagcggg aactgcggca gggcagcgtg agtgaagaaa gccagcagtg
     6481 gcttgcgcag tgcgggctga cggcggaaca gatggccgcg cagctggagg cggaatatat
     6541 cccggagcgg aaacttcatc tttaccactg cgaccaccgg ggactgccgc aggcgctcat
     6601 cagcccggaa ggggaaacgg cgtggcaggg ggagtatgac gagtggggaa acctgctggg
     6661 cgaaaccagc gcgcagcacc tgcaacagcc gtaccgtctg ccgggacagc agtatgatga
     6721 ggagtcgggg ctgtattaca accgtcaccg gtactatgac ccgctacagg ggaggtatat
     6781 cacccaggat ccaatagata taaaaggagg atggaattta tattcttatg cgcttaatcc
     6841 ggtaagttgg attgacccat taggattgac gcagtgcgat tccgaagggt gtaataatga
     6901 tatattattt acaggaggaa gtggtcccga taataaaata cttaatgaat taggtccgag
     6961 agatggcatt gacggtttgg g
//
LOCUS       DBFIFM010000214         6913 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.214, whole genome shotgun sequence.
ACCESSION   DBFIFM010000214 DBFIFM010000000
VERSION     DBFIFM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.214"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(48..2831)
                     /locus_tag="N7C74_004029"
     CDS             complement(48..2831)
                     /locus_tag="N7C74_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGU2433800.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLLQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMEELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(2888..5260)
                     /locus_tag="N7C74_004030"
     CDS             complement(2888..5260)
                     /locus_tag="N7C74_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HGU2433801.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKFKRFNADDSSVKLGYRTTRSDWLTSHINENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGMAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5298..>6913)
                     /locus_tag="N7C74_004031"
     CDS             complement(5298..>6913)
                     /locus_tag="N7C74_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HGU2433802.1"
                     /translation="RKNNKTSVLLIIIILAMILGVVKIQVWLNDWNNDFFNALSQKET
                     DKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIRWREWLTDYYLNRWFADKNYYFTQI
                     YGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQSLSMLITFTVILWQSAGMLSFTVG
                     GTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRPLNVEKQRSEATFRTNLVQHNKQAE
                     LIALSNAESLQRQELSDNFHTIKENWHRLMNRQRWLDYWQNIYSRSLSVLPYFLLLPQ
                     FISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDELAELAAVIDRLYEFHQLTEQRPTN
                     KPKNCQHAVQVADASIRTPDNKIILENLNFHVSPGKWLLLKGYSGAGKTTLLKTLSHC
                     WPWFKGDISSPADSWYVSQTPLIKTGLLKEIICKALPLPVDDKSLSEVLHQVGLGKLA
                     ARIHDHDRWGDILSSGEKQRIALARLILRRPKWIFLDETTSHLEEQEAIRLLRLVREK
                     LPTSGVIMVTHQPGVWNLADDICDISAVL"
BASE COUNT         1771 a         1591 c         1562 g         1989 t
ORIGIN      
        1 cgaatattgc taaaatcccc gccaacgatg tgttgacggg gctgttatta ttttggcaat
       61 aatactccgg tataagtatt taccggatga gaaagatatt gtttaacggc agtgttaaca
      121 ttctctaccg tcatttgttt caacaattgc tcctgctcag tccatgctgc aggatcgtca
      181 tattgaataa gactatttac aatagtgttc gctaattgtt gaacgctacg ctgttggata
      241 tcgaggctgc gctgaacgtt ttgctggtat tcgttcagtt cttgctcact gatcccttta
      301 gtcaaacgct taaccatcac ttcattcgct aacgttaaca gttcatcatg tcgttctggt
      361 tgacaagtaa aagccagcaa atgactgata tctttagcct gaggatcaac cgagaggcga
      421 gaagaaacgc tgtatgcccc agatgcctgt tcacgaatat taacgcgtag atcttttgcc
      481 agtgcgacgt taaaagcatc gagcgccata cgcgtcgcca gattaacagg tgtccgggaa
      541 tcataacgct tccactgtga aacctgtgcc acaggttcat tttgttcctt tacagtaacc
      601 gatgcgttgt ccgtcgcgcg agttaatggt ttacctgcgg ctaatggcga atcagagtgt
      661 ttgattgatc ctaagtaacg cgtaattaac gccacgagtt tgtcttctgc gacattaccg
      721 acaatgacaa acgtgatatc cgctggagat gaaaacaact ggcgatcggc agccagcgca
      781 tctgcggcag taaactgtgc aatctgattt tcttgcagta atttcgtgcg gtcatcagca
      841 tagcgcgtct catacatctg ctgggcgaat ttctccgctg gacgctggtc gagcgttttc
      901 aatgcctgaa tttgagcatt ttgtagcgat gcccaaatat tgtcgttaat cgtgctgtgg
      961 gtgattcgct ggttaatcaa ctgaaaacca ggttcagggt tattagtccg cgcgctaacg
     1021 gagagcaacg tattcatgcc actgacttta ctgctcatgg ttaccgagtt ttccgcactc
     1081 cagcgtttca ggctggaaga ggagagttcg ccaacgccgc ttccgctaac tgctttattt
     1141 gccagcgcga taagtgattt ttgctgcgca gggaaactta aatcgccttt attcgatacg
     1201 gcaataattt gtagcttttg ctcttcaccc gcggattttg ccagaataac ccttgcacca
     1261 ttggaaagtg ttaatgatgt cagattctca gccagcgttt ctttgctgct aatttccgcc
     1321 tgtgggtcag cgtctactgt taacgataaa tttctgcctg ggaagatgta agccgccagc
     1381 tttttgttgg cgtactcctt ttccagcgcc aggatagccg caggagacaa tgcttttttg
     1441 gcggcaacct cattgtttac catttgctcc caaaatgcgt cctggttctt tcttaactgc
     1501 tgccattttt ccgccagact ttgcacggta atttgctgcc acagacgttt cgaaagttga
     1561 tatgtctctt ccggcgacaa gaacggcgta ttatttaatg agctggatgc caggcgactg
     1621 gtcagcatac gtaaatcacg ttcagcttgc tgatcaaccg catttttcag ccaggtgagg
     1681 cgggtagatt tgacatcatc gagttcttca gcagaaaaac catgctgatc aatggttgcc
     1741 aactcttcca ttaatgcatt cgcagcatcc tgcatattat cgtctcgtgc atttacacgg
     1801 aaaaacagcg actgataatc gggtgcaatt ttaacgctgc gcgcagtgcc gccagaaata
     1861 gtcttcaact cgcccgactg tatacgttcc tgcagacgtt gattgaacag ctgaactaac
     1921 atgctccatt cagcttgttc gataaagctt tgctcatcgt tcacttgtac cattggcagg
     1981 cgataataga gtgcgatgcc gttcacccgg ttttctttat cattaatgat attaaagcgc
     2041 aggtggtttt cggcttttgt cggccagacg cgattttcag ctgctttgtt agccggaagc
     2101 ttacttaaat tatcctttat cagcgccagc gcttctttac tgtcgatatc gccaaccacg
     2161 ataaaggtca tattatttgg ttgataccag cgttgataaa attggagcaa ttgtgccggt
     2221 gtgaccgtgg cgacagtatc catcaggccg ataggttcac ggtctaaatt acgggtattt
     2281 gccagcagga aagggcggcg cgcctgagag gtgcgccatt tcgcatcctg atgggcacgc
     2341 cattcctcag taattacgcc gcgttcagcg tctacttcga gtttttcaaa ggttgcggca
     2401 ttactccatt cactgaagat tgccatcact tgttgcagat tttgtttctg cgtagttggc
     2461 aaactcacct gatacaccgt ttcgtcatag ctggtatagg cattaacatc gcgaccaaaa
     2521 cgcaggccca ttgactcaaa tgtttcgatg actttattac ccggccatgt ttttgtgccg
     2581 ttaaacatca tatgttctac aaaatgagcc acgccgcgct cattgtcttc ttcctgcaat
     2641 gaaccggtat gaatttgcag ccataaattt acctgatcct ttggatgagc atgcggataa
     2701 atcatatatc gcaagccatt gtccagttgc ccggtaatta acttttcatc ctgcggtaag
     2761 gcggcggcaa tcagccgccc ggggagcaac agagttgcca ctaacgtcag taagaaacag
     2821 aggtttctca taattatctc catgcgaaaa ccgggcgaat ttacccggtt aagtaaaatc
     2881 cgaactatta aaatttcatg ctgacatcaa gccagaaagt acggccactg gcgtaggtcg
     2941 ccacaccggt attcgttgta tcaaccgcgg ttttgctatc cagtacattg agaatatcgg
     3001 cactgatcgt caggttttgt tgtttcagaa attgtggcgt ccaggacaag cgggtgtccc
     3061 aggtcaggct gctatccagt ttttcgtcaa catactgctt gtaatctgaa tattcgctga
     3121 tgtattgcgc atttgtctta cccaggataa tgcgagcttt acgcgcttct tgccaggcca
     3181 gcgtattcgc ccacaccaac ccgctcggtt gatgcgtgaa atccatgttt aaggagatct
     3241 ttaatgggtt attaaaatct gccactggaa cgctatcgta agagacaaga ttaccgttat
     3301 aaaccacctg gttatcaccg gtattgctct cctcataacc attgtttaac gacaagttgc
     3361 ccttgctctt gatatagcta aagacaattt gcgggttaat atctacctgg ctgatatgca
     3421 ggggttcggc cagttcaaaa ctgaggttga acgaatgcgt tttggttttg ccgtcgttgt
     3481 tatattcagt aatggtggtt ttagtcgcgc tgtcggtacg actgcttttg ctgatttgat
     3541 catgcgcttc acggtaaaca tagtttgcgc gcgcaataac gttcttaccg attttttgct
     3601 gcaatcccat tgccagttca tcgttataag gcgttttcaa atcctgataa cgcgtcaggg
     3661 ttttattacc tgataccgat tccgtccagc tattgcggat atcacgtaat cccatatcaa
     3721 gaatattccc gccatagtaa cggttataac ctgctgtaat cattgaggtt tgatcagcaa
     3781 aaatatccca ttccgtcata aagcgcgggg agatattgtg gtttgacaga tagttgtcat
     3841 agtcataccg cacgccgggc attaatgaca cattacgcca gctaatgcga tccgccatat
     3901 acagtgtgta gttgtcaatt cccaggctgc ctttaccttt atggtaaatg gtatggttag
     3961 ttttctttcc ggcagcatta atcacatagg attcagactg gttatggcgt tcagtccacg
     4021 catcggaata gatgtattcc gcgccgaagt agggttgatg cgaaacatta cccacggcga
     4081 atttttgcca gtccaggcgt gttttgaagg tgtaattatc tacagcctgg gaaatgtgtc
     4141 ctaatccgcc acgggtgcaa cgccctgtaa tatcaccata tgtacatgaa agttcggtgt
     4201 accagatgtc atgatcgtga cgggtataat cactaatatg atcccaacca acggtggtac
     4261 gtagtttggc ccatgcgagc tgcgtatcca tatcccatgc cataccatag gatttattac
     4321 ccatttcgcg atcagactgc gggaaggtgc tggtattata atcacggctg gagccggtat
     4381 attttaaggt taaatcgtgg gtaaagcggt cgctggcaaa ccaggtaaat ttgctcaatg
     4441 cagtatcgat aacgttttta tactgtgccc gaccggcgac aatgccgtca ttcgaaacat
     4501 aatccgcgcg ggtgatatca gactggcggc gcgataaacc ggcggtaacg ccaaagttat
     4561 cagcgagttc ctgattaaac gacaaggtat aaaagttctt tttaaaatct ggggagtaat
     4621 aagtacttcc tgaagagcct tgattaaatg cgctcttgtt attctcattg atatgcgatg
     4681 ttaaccagtc cgaacgcgta gtgcgataac ccagtttcac gctgctatca tcagcgttga
     4741 agcgtttgaa ctttgcatca attaccccgc cattgaagcg accgaattca accggcacaa
     4801 aactgtcata aagcgtcaca ttgtccagta agctgacatc aagataatac ccctgtgaca
     4861 tcccgctaat attggttgca ctactggcat cggactcatt cgctgggttc aggttattag
     4921 tggcactaat accgtcaatc aaataggcat tctgataggg cgacgcaccg tgaatagaga
     4981 ttttctccgg gcgaatatct ccctggttca acgaggtact ttgcgttgaa tccatgcgta
     5041 ccgcagggtt ggttctcagc agatcgctga tattgccatt cccggtcggt aaacgctcga
     5101 tgctttcact ggtgtaatgc gtgttgccgt ttacgggtac agggacagga gcatagaccg
     5161 tcatctcttc aaaaaaatgc atgtacatgt ttttgtcatc gacggcctgc gccacatcag
     5221 cgccacataa aatgacgcca ggaataagaa ctcgcttcat ttatttatat ctccgtacag
     5281 cagggctttt attgttttta taaaaccgcg ctaatgtcac aaatatcatc ggccaggttc
     5341 cagacaccgg gttgatgtgt aaccataatg acgccgcttg tgggtagttt ttcacgcact
     5401 agacgcagta agcggatagc ctcttgttcc tcaagatgag aggtagtttc gtcaagaaat
     5461 atccatttcg gacgtcgtaa aattaatcgt gccagggcga tacgttgttt ttcgccgctg
     5521 gaaagaatat ctccccagcg atcgtggtca tgaatacgcg cagccaattt cccaagacca
     5581 acctgatgca gtacttcgct caatgattta tcgtctacgg gcaggggaag tgctttacaa
     5641 ataatctctt tcagtaagcc ggttttgatt aacggtgttt gtgacacata ccaactgtcg
     5701 gcaggagaag aaatatcacc tttaaaccac ggccagcagt gggataatgt tttaagcagt
     5761 gtggtttttc ccgcaccaga gtaacctttc agtaatagcc atttgcctgg cgaaacatga
     5821 aagttcaggt tctctaatat gatcttatta tcaggagtac gaatactcgc gtcagccact
     5881 tgtaccgcat gttggcaatt tttaggctta ttcgtagggc gctgttcggt gagttgatgg
     5941 aactcataca agcgatcgat aaccgcagcc agttcagcaa gttcatcata tttataaata
     6001 aaccagctta gattgttcga taccagcata aatgcctggc gcgatttcat cagtccgccc
     6061 agattgatct gcccgctaat aaactgcggc aataacagaa agtagggaag aacgctaagc
     6121 gagcgcgaat aaatattctg ccagtaatca agccaccgct gacgattcat taaacgatgc
     6181 cagttctctt taatggtatg aaaattgtcg ctcaactcct ggcgttgtag agactcagca
     6241 ttgcttagcg caatcagttc tgcctgctta ttatgctgca caagattagt tcgaaatgtc
     6301 gcttcgctac gttgtttttc cacattaagc ggacgaatac gtttacccac cttatgggta
     6361 aataaagttc caccgatcac aatgagcaca acggtataga ccatataccc ctggatattc
     6421 cattctgttc caccaacagt gaatgagagc atacccgcgc tttgccaaag aataacggta
     6481 aaggtgatca gcatgctaag tgactggatg aagccaaatg aaagactgag cgttttgctg
     6541 atcagtaaga gaatgtcttc agcgatacgt tggtcagggt tatctgtatt tttatgctcg
     6601 ccgtagatct gcgtgaagta gtagttttta tctgcaaacc agcgattaag gtagtaatct
     6661 gttaaccatt cgcgccagcg aatagtcagt agtttaatta accatgtttt gtttacggat
     6721 attaatacaa agatccccag cagggcaggg aaccaaagga caagctgcca aagcttatcg
     6781 gtttctttct ggcttagcgc attgaaaaaa tcattattcc agtcgtttaa ccagacctga
     6841 attttaacca cgccaaggat catggcgagg ataataatga ttaacaacac tgaagtttta
     6901 ttattcttac gca
//
LOCUS       DBFIFM010000215         6896 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.215, whole genome shotgun sequence.
ACCESSION   DBFIFM010000215 DBFIFM010000000
VERSION     DBFIFM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.215"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..2372)
                     /locus_tag="N7C74_004032"
     CDS             complement(<1..2372)
                     /locus_tag="N7C74_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU2433803.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDVRKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLIAMVRQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTASFRANLQGAWNDRENARLGLAAATLQSDMEKAGELAARD
                     RAERDASQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILRADYNTLMAAAK
                     KDYESTLKKPKSSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANEKISQQRRDL
                     WKAENQYAVLKEAATKRQLSEQEKSLLAHKDETLEYKRQLAELGDKVEYQKRLNELAQ
                     QAVRFEEQQSAKQAAISAKARGLTDRQAQRESEAQRLRDVYGDNPAALAKATSALKNT
                     RSAEEQLRGSWMAGLKSGWGEWAESATDSFSQVKSAATQTFDGIAQNMAAMLTGAEAD
                     WRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAFGGGASASSGTAIQAAAANFHFA
                     TGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVG"
     gene            complement(2353..2766)
                     /locus_tag="N7C74_004033"
     CDS             complement(2353..2766)
                     /locus_tag="N7C74_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU2433804.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            complement(2793..3224)
                     /locus_tag="N7C74_004034"
     CDS             complement(2793..3224)
                     /locus_tag="N7C74_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU2433805.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMSGAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            complement(3238..3978)
                     /locus_tag="N7C74_004035"
     CDS             complement(3238..3978)
                     /locus_tag="N7C74_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2433806.1"
                     /translation="MPLEPVKGAGTTLWVYNGQGDAYANPLSDDDWQRLAKVKDLTPG
                     EMTAESYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFESGDVR
                     AYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITPVSAI
                     KVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNGVATG
                     KVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            complement(3975..4226)
                     /locus_tag="N7C74_004036"
                     /pseudo
     CDS             complement(3975..4226)
                     /locus_tag="N7C74_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
     gene            complement(4284..4631)
                     /locus_tag="N7C74_004037"
     CDS             complement(4284..4631)
                     /locus_tag="N7C74_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGU2433807.1"
                     /translation="MVTKNITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EASGKLVVWDGQKAGSAVGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            complement(4668..6173)
                     /locus_tag="N7C74_004038"
     CDS             complement(4668..6173)
                     /locus_tag="N7C74_004038"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGU2433808.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPAEVRQNMQQRIDAARRMFAEKVAMFTGLSVDAVTGTEAAVFEGQSGIDAGLA
                     DELVNASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASTQSEADRIMACEEANGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLA
                     AGSGKAEPVSASTTALFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            complement(6163..>6896)
                     /locus_tag="N7C74_004039"
     CDS             complement(6163..>6896)
                     /locus_tag="N7C74_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU2433809.1"
                     /translation="AFEYIAGAPQGQKDNPLINILEKFSSWYDTNNVTLGGVKIPHLF
                     PGDDLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANES
                     WRYFMGRRKFIAARLATQMFSYWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAG
                     RMAIDGLKEVQESVMRIEAGLSTYEKEPALMGEDYQDIFRQQVRESAERQKAGLSRPV
                     WIAQAYQQQIAESRRPEEETTPRET"
BASE COUNT         1379 a         2247 c         1708 g         1562 t
ORIGIN      
        1 cccacataac caccttccgc atagccgcgc atcagacggt aaagattccc cacacctatc
       61 cggctggttg cctctttcgt gaaaacaaac tccccgcggt gaactatccc cgcaggctca
      121 tatttgccgc ccgtccccgt aaatcctccg gtcgcgaaat ggaagttcgc cgccgcagcc
      181 tgaatggccg tccccgagga agcagatgca ccaccaccga aagcaccgcc aatggcgctg
      241 ccgatacgcc cgacaatgcc caccatggcc tgtttaagca ggatttctgt catcatggac
      301 agcaccgaac gggtgaatcc ccgccagtct gcctctgcac cggtcagcat cgccgccata
      361 ttctgtgcaa taccgtcaaa ggtctgcgtg gcagcacttt taacctgcga aaaactgtcc
      421 gtcgcacttt ccgcccactc gccccagccg gacttcagcc cggccatcca gcttccacga
      481 agctgctcct ccgcagacct ggtgttcttc agtgccgatg tggccttcgc cagcgcagcc
      541 ggattatcac cgtacacgtc ccgaagacgc tgcgcttcag actcccgctg cgcctgacgg
      601 tcagtgagac cgcgggcttt tgcgctgatg gcggcctgct tcgcgctctg ctgctcttca
      661 aaccgcaccg cctgctgtgc cagctcattc aggcgtttct ggtattcaac cttgtcgccc
      721 agctcagcca gctggcgttt gtactccagc gtctcgtctt tatgcgccag cagggatttt
      781 tcctgctcag ataactgccg tttcgtggca gcctctttca ggaccgcata ctgattttcc
      841 gctttccata aatcgcgacg ctgctggctg attttctcat tcgcaccgct gtgtttttcc
      901 agcgtcctga gctcggtttc aagcgccagc agggcagcat gcgcctggtc ttcctgacgc
      961 tcaccggctg acactttgac tcctgacgac ttcggctttt tcagcgtcga ttcataatcc
     1021 tttttcgccg ccgccatcag cgtgttgtaa tccgcccgca ggattttccc gtctttcagg
     1081 gccttattca gttcttcctg acgggcggta tatttctcca gcggcgtcag cagacgctca
     1141 tacgccttct gcgcctctcc ggtatacttc agctgtgatg cgtcccgttc ggcccggtcc
     1201 ctggcggcca gttcaccggc tttttccata tccgactgca gcgtggccgc tgccagcccc
     1261 agacgggcat tttcccggtc attccatgcg ccctgaaggt tggcccggaa agaggcggtt
     1321 tttccccggc gctggctccg gctctggtac cactgccatt ttttatccgc ctcatcaaat
     1381 gccttctgtg cactggcgag catatccgct gaggactcag gacgaccgat atccagaatg
     1441 gcatcccaca tcgatttgaa tgccttcccg gttttatccg cccaggtctc cagtgtcccc
     1501 atgttttctt tcaggcgacg ggtctgctca tcaaagcctt tcgtggcgat atcgttcgcc
     1561 gcctgtaagg ccccggcctc atcaccggaa cgctgcagct gcgcaacata cgcaatctgc
     1621 tctgccgtca cgttacggaa ctggcgcacc atcgcaatca gccccgacgt agggtcggtg
     1681 gtcagttttc cgaaagcctc tgcaaccttg tccacctcca caccggatgc agaagcaaaa
     1741 cgcgcgacac tctggttgat ggcatcaaac tgttcaccac cacgcacacc ggcattcacc
     1801 agggctgcca gtgactctct cgcctggtta aacgtcagcc ctgctgcctg cccggctctt
     1861 gagagcgtca gcatgcgatc ggcagtcagt ccggactgat taccggaaag aaccagggtt
     1921 ttattaaacg ctgaaagcgt ggaatctccc tggtaccagg cgtacaccag cgcacctgtc
     1981 gccaccgcca gcgaggtgac cccgaccatc ggcagggtga tcgcaccggc aagccccctg
     2041 aacatgggga tcatcccgcc gaaggagtcc ttcacctgac cgccctgttg cagcaggatc
     2101 agccagggat tctgaccacc ggcaagctgc gtggcgatat ccgtaaactg tgcgggcagg
     2161 gttcgcatgg ccgctttata ctgcccgacg gaaatcccgg ctttttgtgc agccagcgcc
     2221 tggcggctca ggccctgttc aacagcactg gcggtttttc tgacgtcggt atccagacct
     2281 gaaaaatgac gccttacccg gctcatctgc tcatcgaaac ggacagcatc cagactcagg
     2341 tcaataacaa gatcaccaac cggctgggac atatctcaca cctcccggaa tccccgctga
     2401 agccatcatt aatgcggcat catccaccat gacatccgcc acatccgcag acgataaaat
     2461 atcgcgccct ccgtccccac cgaaccggac gcctccggca agtcctgccg ctttctgcat
     2521 cagcattttg tcctcatccg gcctctccac ctgctcttcc tcatgccggg ggacaagcag
     2581 actgaaatca gagggatgca tatccggatc gcaaaaaaac aggctgagta cagcgtacgt
     2641 cagcccggaa aaatgcatat ccagctgggt atcctgaaaa taatgcgtgc ggtaaaaacg
     2701 gtgccagtcg gcatattcgg tggatgtcat cccggcaagc atggcgcgcc agtcgggtct
     2761 ccccatctca cgcgccagtc tgagggcaaa gttcagctcg ccgtcgaaga ctttcccgca
     2821 gaaaaatcat catcagtcag cgtgttattt ttcgccactt cagtaatatc agtatccgga
     2881 cgaacagccc cgctcatccc ggacaggcac aacaccacgt cttccgcccg ggcaatggca
     2941 tcggcaggcc aggtggtgag cacttcctgc tctatcttca tcacggcctc attcattgac
     3001 ggtgactgcg ttttctgtgg atggttatgc cacagggaca tcgccaccag aaacgcgccg
     3061 gttctgacga gatcttccac gcttacctgc aggttgccgc tggattctgc ctgttctgca
     3121 cgccgtttca ggagggcaag atgctcgata cgctgcagcg cagacaattc ggaaagcgtg
     3181 acagacacac cgttatattc aaattgttct gttttcagaa acatgtatga cctccgttta
     3241 ccctgcagcg cccgcttcag taacggtgac ttcagccact gcggcgaact gaccatttcc
     3301 gctcaccaca gggatctgca ccttacctgt cgccacgccg tttaccgtaa ttgtcatatc
     3361 tttcacacta atggtggctt tcgacggatc ggcggaaacc gctctgaacg tcttgtctgt
     3421 tgcactttcc ggctcaaaag aaaccgtcag ggtggttgtt ttcccttttg ccaccgtacc
     3481 ggatgtcggc gtcaccttaa tcgcactgac cggcgtaatt ttgctgcgtt cttccgctac
     3541 agaaggttta cccacgttag tgactttcac cgtgcgggtg atcacttctt tcgccgtcac
     3601 ggccttaccg atactgctga cccagccacg aaacacatcc accgtgccat tcggaaaacg
     3661 gattttatag gcccggacat cgccgctttc aaaccagcct ataagccctt tctggccttc
     3721 ctctcccggt ttccaggcca gcgtaaaact ggtatcacct gcagatttct gcccctgccc
     3781 ggtcgcggtc cagtccgcgt cttcatcatc caggtagtta tcatcgtagg attctgccgt
     3841 catctcgccc ggcgtcagat ccttcacctt agccagtcgc tgccagtcat cgtctgacaa
     3901 cgggtttgca taggcgtcac cctgaccgtt ataaacccac agggtggtac cggctccttt
     3961 taccggctcc aggggaattc gtgtttcggc ttgacgtagc ccggacgtaa cacgcgggtt
     4021 tcaccaccgc gatgacgcag cacttttcct tcaacaaccg gggagacata ggccgccacc
     4081 ggcgtttttc cggtaatttt gtccagcatc acctcttcgg tatggaaatt caccgtacgg
     4141 cggaaaaaca gctccagaaa cagcgcacga aatttcactt tttgttcggt ataaccgagt
     4201 aactggcggg tcgtaaacaa tcccataaat cagttccttt cattcagaaa tcagtcaggc
     4261 caccgcggtg gcctgataac gtgttacggc agcgccgcgt gactcagggc actgccggca
     4321 aaggcatttg cctttttgtg ttcatccaca ctttcaggcc agcggattgc ctccgtcgca
     4381 aaggtccccg acttgtaata ggtcagtacc gtctctgtgc cttcaagcgg cagtaccagt
     4441 atgccaactg cactaccggc tttctgtccg tcccagacca ccagtttccc gctggcttca
     4501 tccagcatca ggggcgtcag tgccggtgtt gccgaggaaa tcccgctgct gcctgtggcg
     4561 gtatgagccg gatcattacc ggcaaaaata cgtacttccg cacgctgttc agtgatgttt
     4621 ttcgttacca tattgtaaaa acctcctgtt gatggtcagc actgacttca tggcatggcc
     4681 atgagcattt tcacgtccgc atcaccgtct gctgacgtct gtggcacgcc accctgtacc
     4741 gcagccggtg aatggttcgc catgatgcgt tcaaacaggg cggttgtgga tgcagagacc
     4801 ggttctgcct tacctgatcc cgcagccagc acagcccggg cgctctccac agtcattccc
     4861 gggcaggcag ccagctgttc agcctgcgcc tcagcccctt ttgcctcatc cagtgccatg
     4921 atctgatcac ggagtgaggg tccggcatcc gcctgcggtg aagcagccag gatcgggcgg
     4981 gctttttcca ccgtcatctc cggcatcgcc gccagcgttg ccgccagttg ttcacgaccg
     5041 ttcgcttctt cacacgccat aatgcgatcg gcttcactct gcgtggatgc caccggctgc
     5101 tgcggtgccg ccgcggccag aatcgcccgg gcctgttcaa cgctcatgcc ctgttgtcct
     5161 gccagcatcg tggcaagctg ttcacgtcct ttcgcttcct ggcatgtcag gatccccatc
     5221 actcgctggt tctcctgcgc ggcggcttcc gttgcagtta attgcggcat agtgcctcct
     5281 ctgacattac tgttcagcgc cgtggccatc acactgatgg catccgacgc attgactaat
     5341 tcatccgcca gcccggcatc aatgccggac tgaccttcaa aaacggcggc ctctgttccc
     5401 gtgacggcat caacagacag accggtaaac atggccactt tttcggcaaa catccggcgc
     5461 gccgcatcaa tgcgctgctg catgttctgg cgaacctctg ccggtaaggc ttcaaactga
     5521 ttgccatcca ccttgtgcgc ccctgagtaa atcagcgtga tatccacacc ggcctgcgcc
     5581 agatgaccgg catagctgac atggctcatc atcacgccaa tggagccgat acgggatgtc
     5641 tgggtaacca gccgtcggga gcaggccgac gccagcagca tggctgcaga acaggccgtg
     5701 tcattgcaca gtgcccagac cggcttctgc tgacggaggc ggtaaatcat gtcagcgcag
     5761 tcaaacgcgc cggcggcctg cccgcccgga ctgtcaatgt ccagcagtac gccccgcacc
     5821 tggctatccg ccattgcctg ctgaagacag gcgacaatgc cgtcatagcc tgtcattccg
     5881 gaaaatggcc gcataccccc cagccggtgc accagcgtgc cggtcaccgg cagtacagca
     5941 ataccgttca ccacccggta aacacgggcc ggtcgtttac ctccggccat gtactcgtcc
     6001 gtttcagcca gcattccggg agcatcaagc tgtacctgct gctgtggtac cgaaagactt
     6061 gctgccccca tctcgcgccc gagcgcgcaa aagaaaaccc gcgcataggc gggctccaga
     6121 agcagcggtt cattgaatgc tgcggcaata atgtgtgaaa gattacgtct cacggggtgt
     6181 tgtctcctct tccggcctgc gactctccgc tatctgctgc tgatacgcct gcgctatcca
     6241 caccggacgt gagagtccgg ctttttgccg ctctgcagat tccctgacct gctggcggaa
     6301 aatgtcctga taatcctcgc ccatcagcgc cggctctttc tcatacgtgc tcagtccggc
     6361 ctcaatgcgc atcaccgatt cctgaacctc cttgagcccg tcaatggcca ttcttccggc
     6421 accaatccac tctgcccgtg accaggctga tcgcgcctga taaaaatcaa aacgcgcccg
     6481 tggcggacgg ataatccccc gaagaagtgc ctcttccagc cagtaggaaa acatctgcgt
     6541 ggccagccgg gccgcaataa attttcgccg ccccataaaa tagcgccacg actcattggc
     6601 agaggccctg gcacttgaat aactgacctt cgagtaatca cgggacaact gttcgtagga
     6661 aacgccaaga ccggcggcga tataccgcag cagcgcctgt tcaagcgccg aaaatccatt
     6721 gtctgaatcc tgcgcagtct gtagtttcag atcatccccg gggaaaaggt gcggaatttt
     6781 gacaccaccc agcgtcacgt tattcgtgtc ataccagctg gagaacttct ccagaatatt
     6841 aataagcgga ttatccttct gcccctgcgg cgcaccggcg atatattcaa aggcct
//
LOCUS       DBFIFM010000216         6879 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.216, whole genome shotgun sequence.
ACCESSION   DBFIFM010000216 DBFIFM010000000
VERSION     DBFIFM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.216"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(50..457)
                     /locus_tag="N7C74_004040"
                     /pseudo
     CDS             complement(50..457)
                     /locus_tag="N7C74_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152817.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(691..1431)
                     /locus_tag="N7C74_004041"
     CDS             complement(691..1431)
                     /locus_tag="N7C74_004041"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HGU2433810.1"
                     /translation="MAERLREIQKQVITVCDREADIWHYLHYKVSRGKPHAECDAKRR
                     AGSPPGPDAHQLQRSQHKKSRQQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADA
                     RRIVSHYEQRWLIEEYHKAWKSGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGGISE
                     ETQNDSCEKILTPTEWKLLWVKLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPG
                     WVVMWDGWFRHQDMAEGYLVMKSLDQEI"
     gene            1360..1824
                     /locus_tag="N7C74_004042"
     CDS             1360..1824
                     /locus_tag="N7C74_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433811.1"
                     /translation="MPDIRFPVTDSDYLFLYLPQPFGHALRSLLPAVTFFFFTGTFFS
                     LVLNTVGVRGTTPALLFDKIDWQGAVGGVHQDRVSQQPVRLRVSGGIPQVIKCRTVTE
                     VNGGGIFQREEQRMCLTCPCSGSKSGFGNGLRGHRRVTYQAVRTFNLSSEVG"
     gene            complement(1756..1959)
                     /locus_tag="N7C74_004043"
     CDS             complement(1756..1959)
                     /locus_tag="N7C74_004043"
                     /inference="COORDINATES: protein motif:HMM:NF026057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HGU2433812.1"
                     /translation="MVLSDCYSLANEQSGHARLGDPRRTRRLVSLTSSLAQHAGLSIV
                     KSSHFTAQVEGAYRLIRNPSVSP"
     gene            2134..2310
                     /locus_tag="N7C74_004044"
     CDS             2134..2310
                     /locus_tag="N7C74_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433813.1"
                     /translation="MVNKTANVAAHAVVNAALAVVQGNNARASAAGAATGDVVGMIAT
                     EMCGISPGTVTCKR"
     gene            2871..3431
                     /locus_tag="N7C74_004045"
     CDS             2871..3431
                     /locus_tag="N7C74_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU2433814.1"
                     /translation="MISVMILYVMSGSRLADNHTLSAGYAQSKVQDFKNIKGVNLQYR
                     YEWDSPVSVVGSFSYMKGDWADSHRDEADDFYRHQADIKYYSFLAGPAYRLNDYISFY
                     GLVGISHTKAKGDYEWRNSVGADESDGYLSESVSKKSTDFAYAAGVIINPWGNMSVNV
                     GYEGTKADIYGKHSVNGFTVGVGYRF"
     gene            3567..4358
                     /locus_tag="N7C74_004046"
                     /pseudo
     CDS             3567..4358
                     /locus_tag="N7C74_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
     gene            complement(4363..5733)
                     /locus_tag="N7C74_004047"
                     /pseudo
     CDS             complement(4363..5733)
                     /locus_tag="N7C74_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(5753..6100)
                     /gene="tnpB"
                     /locus_tag="N7C74_004048"
     CDS             complement(5753..6100)
                     /gene="tnpB"
                     /locus_tag="N7C74_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU2433815.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDAPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            complement(6097..6771)
                     /gene="tnpA"
                     /locus_tag="N7C74_004049"
     CDS             complement(6097..6771)
                     /gene="tnpA"
                     /locus_tag="N7C74_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HGU2433816.1"
                     /translation="MNSQTKKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFIASGIAWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRTSYSRE
                     FKIRLVKQALQPGAVVARIAREHGINDNLLLKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKPDECPESDPGNVPRCELHLKSGVVKLFDPLTPEMLRALIRE
                     MKGGTR"
BASE COUNT         1632 a         1603 c         1795 g         1849 t
ORIGIN      
        1 tgtacagtac cagatactca gccagtttca gattgaacat attcagctat tcaaagagca
       61 attcttcatt aaactcaata aattgttccc tcagtgttcg gttaaaatgc tcacttatgg
      121 catttatttt cggcgtatag agataagtcc atacgaaccc gacggtggct tcctgtagcg
      181 ttttgtcgaa ttttcccgag aactctttgc ccatcatcag tgatgatctg gttgatacca
      241 acaaggaaca actgggctac acagcagaag aaatgcctga tgatatcact tttaagcgat
      301 agtacggcca gagagtagtt gctgtgttcg tcgagcatgg taatgaaata acggtacagc
      361 tcgccattct gagctcaata gtatccatgc cactgagctt acaggtcttt atcgggcgat
      421 agtgctttat tcttcggggc tttgtggatg atttttcgaa atgttcatga tagttaggta
      481 aagctaccgc atatcatttg caaaatgaca gatagatata cttcaatttg aatcttaaca
      541 attagtatgg gaatgctgtc aatcctctgt atgcgtccac cacatattga aatttttgtt
      601 ctctaatata ttccacgggc gtcaagagtg tgaggcctgt gctgttatac agcaccagac
      661 gttgtccccc atctttgacc tgtctcttga tcagatctcc tgatcaagag acttcatcac
      721 caggtaaccc tcagccatat cctgatgtct gaaccagcca tcccacataa ctacccaacc
      781 ggggctgctt gtgcgtttgc tgtcatgcca tcgccccagt ttagccagtt tcaaacaggc
      841 ccatttcagg gtcggtgttt gcgccggaag ctgctttcct tccagcttaa cccacagtag
      901 tttccactct gtcggcgtca gtattttctc acagctgtca ttttgtgttt cttcactgat
      961 acctccctgc cgcaggccca gcacccgcac cgcgataacc accatgcgct caaggttatc
     1021 ccgggtctgc attcgcagcg attccacaca tgtaccacca cttttccacg ctttgtggta
     1081 ttcctctatc agccagcgtt gctcgtaatg gctgacgata cgtcgcgcat cggcggcact
     1141 cgccactttt tctgatgtca gcggatacca gcaggctccg tcctctcctg ctccccgcaa
     1201 cagacatacg tgagcgagag cgcctggccg ctgttgtcgg gattttttat gctgacttcg
     1261 ctgtaactga tgagcatccg ggcctggcgg gctgcccgcc cgcctttttg catcacattc
     1321 agcgtgtggc ttcccgcggc tgactttata atgcaggtaa tgccagatat ccgcttcccg
     1381 gtcacagaca gtgattacct gtttctgtat ctccctcagc cgttcggcca tgcgctccga
     1441 agcctgctgc cagcggtaac tttctttttc ttcacaggga cgttcttttc gctggtgctt
     1501 aacaccgtag gtgtccgtgg cacgactcca gcactgctgt tcgataagat cgactggcag
     1561 ggcgctgtcg ggggcgtaca tcaggacaga gtgagccagc agcccgtgcg tcttcgggtt
     1621 agtggtggta ttccccaggt catcaaatgc cgtactgtga ccgaagttaa tggtggtggt
     1681 atcttccagc gcgaggagca gcggatgtgc ctcacatgcc cttgcagtgg cagtaaatcc
     1741 ggcttcggca atggcttacg gggacaccga cgggttacgt atcaggcggt acgcaccttc
     1801 aacctgagca gtgaagtggg atgatttcac aatagaaaga cctgcatgct gagcgagaga
     1861 agaggtcagt gacacaaggc gtcgtgtacg acgcggatca ccgagacggg catgtccaga
     1921 ctgctcgtta gccagtgaat aacaatcaga aagtaccata acagacccga agaaaatgaa
     1981 atataagaga agatcaacgg gtgaagaaaa agttcaaaaa atcgctgccg gggaggaagg
     2041 aaactaccgg atggaaagag tccccctaaa gcagactgac agacatcaca aatccccggg
     2101 tggggatttg tgtataagag acaggttaca ggtgtggtga ataagactgc aaatgtggca
     2161 gctcatgccg tggtgaatgc tgcactggcg gtggtacagg gaaataacgc ccgtgccagt
     2221 gccgcaggtg ctgccactgg agacgtggtg gggatgatag ccacagagat gtgcggaata
     2281 tcccctggca ctgttacatg taaaagataa ataacgaagc tacatccttc tctttatgaa
     2341 cggctttata ttagttttgc tgacaagatt gagctaaatc cggccaatga agaagagcag
     2401 gttattcttt aaaggtttga taacatgtaa cacattttga atattcagtc agtaagcctt
     2461 aaaaatttac ctggttatgg gctgactgat ataacaaagt aaatattcga tggggaatgc
     2521 cggatgcaat gaacaatgca ttaacgtatc gaaataatga tgcaggagca aactcagtct
     2581 ggtgagtata cattatgcca ttgatgcaga gtttaaagga acagggctgg ttcgttatgg
     2641 aacaacattt gtgcctgagg aaaggggggg gagtatctga ggcaacagaa gtttgtgggg
     2701 agttcggttt attaccatgc ttggtttttt gtcagtgtgt tcaaatcgtc aggtgatata
     2761 tttatagttt tttgatgatg atttcaaata atgtttttga gatgtgtggt cctttttgat
     2821 tttcagaaat catttattaa tgaaggaggg ttaaatgaaa aaaactcgtt ataatttcag
     2881 taatgatatt atatgttatg agtggttcaa gactggctga taatcatacg ctatcggctg
     2941 gttatgctca gagtaaggta caggacttta agaatattaa aggagtgaat ctgcaatatc
     3001 gatatgagtg ggattctccg gtcagtgtcg tcgggagttt ttcgtatatg aaaggtgatt
     3061 gggcggacag tcatcgcgat gaagctgacg atttttacag acatcaggct gatattaaat
     3121 attattcatt tctcgcaggc ccagcttatc gtctgaatga ctatatcagt ttttatggtt
     3181 tggtggggat atctcatact aaagcgaagg gagattatga gtggagaaac agtgtgggtg
     3241 ctgatgagtc tgatggatat ttaagtgagt ctgtatcaaa aaaatccact gattttgctt
     3301 atgcagctgg tgttattatt aatccgtggg gcaatatgtc tgtaaatgtt gggtatgagg
     3361 gaactaaagc agatatatat ggaaaacatt cggtcaatgg atttactgtt ggagttggtt
     3421 atcgttttta atcattagac gggactggat aattgcactt gcgtagtgaa gtttactaaa
     3481 tgtttgtatt ttaatgaagt attcgacata ttcccttcag acggaatatg tcgacggtta
     3541 tggttttact ggagcatgag tatgttatgg cgaaactgat aaatatatca gcgtccactg
     3601 aggagagaat gacgccaggg gagaggaggg tggcttcacg actggagagc tttctgaatg
     3661 atgattgctt tgtctggtac gatatcccgg tgggacggaa aaaccgacat ccggattttg
     3721 tcattattga cccggataac gggctggtat ttctggaggt caaggactgg actgtatcca
     3781 cattacgtaa agcaaatcag gagcaggtta cactggaaac cgacggtctt ctgaaatcag
     3841 aaataaatcc gctggtacag gttcgtcgtt atgcctgtga tacggttaat gccttacctg
     3901 ctgacccgtg cttacggcag aatgacggtc agtataaggg gcggctgaac ctggcctggg
     3961 cgtatggtgt ggtgtttacc cgcattaccc gtcagcaact gaaggcactg acagggaata
     4021 atgagaatgc ggtggaaaag atttttcctt cagcacagac aatctgtcag gatgaaatga
     4081 cgcagtctgt tttacctgag gtattccggc aaaaaattgc cgggatgttt accacggggt
     4141 tcagaacccg ggtaacaccc cggatgaggg atattctgcg cgcccatctg tttcctgaag
     4201 tgaccgtgaa acagaactca caaatcaaaa ttatggatat tcagcaggag attctggcca
     4261 gaaatattgg tgacgggcat cgtgtcattc atggtgtggc cggttccgga aaaaccatga
     4321 ttcttttgtt ccgttgcctt tacctggcag aaaccacgtc cggtagttac gccgccccag
     4381 tgcaactacc cggagggcgt tttcacacag gttattgtcg atctccaccc agccattgcg
     4441 gcagtattcg ttcagagcgt cccattgctt cagcagatag gtgaacgctt tcgctgtatc
     4501 cgagtggcgc gacagcgtgt tcatctgccc ctagagccac tcatacaacg actgcattag
     4561 cggtaccgtt ctggcttttc tgaccgccag tcgctcttct gccggactgc cgcggatctc
     4621 agcctcgata gcgtacagtt ccccgatacg acggagcgct tctcccgtta ctgtcgttgg
     4681 atggcggaca tgtacatcgt ggatcttgcg tcgggcatgt gccatacaag ccgcttcggt
     4741 tgcctgaccg ctttcgtaaa gagcattgta acccgcatat gcatcggcct gcaggatacc
     4801 tctgtagtcc gccagatgtt gctgtgggtg gatgcctttg cggtcgggag agtatgagaa
     4861 ccacaccgct gccggcatgg tcgaaccggc gttgcgatcg tcacgaacat agacccacag
     4921 ccgtccggta cgggttttac cctgacccgg ctccagtaca tttaccggcg tgtcatcggc
     4981 atgcacttta cccggcatca gcacatagtg cttcagttca tcatacagcg ggcgaagctg
     5041 ctctcccatg atgtcaaccc agcgccccat cgtattgcgg tgcagctcca cgccctggcg
     5101 ggcatagatt tccgactgac ggtacagcgg cagatgctcg gcgaacttag ccatgattat
     5161 gcgggccagc agagccggac tggcgtaact gcgctcgatg ggttttggtg gctgcggagc
     5221 ctgaactata cagtcgcacc ggctgcaggc ccgttttggg cgaaccgttt cgattaccct
     5281 gaacgcggtg ttgatgatat ccagttgttc agagatgctt tctcccagcg gtttcagttt
     5341 accgctgcag acggggcatt cggtttctgc cggggagata acctgcctgt cacggggaag
     5401 tgttgccgga agtgctttgc ggacgggaga gtctgatgtt ttcggcgctg tctctccggc
     5461 cattgaggtg agttgcaact gcgcctcacc aagcctgttc tggagctcgg ttatacgcgt
     5521 ttctgcccgt gcgatcttct tttctatctt ctcgcggctt ttctcgctgc tgcgaccgaa
     5581 caacattctc tgcagtttag cgaccagcgc tctgagtgag ctgatctcgc gggcatagcc
     5641 ggttatttca ccagacagac ggacgataac agcctgctgt gcgatcagca gggccttcag
     5701 ttgctcgatg ttgtcgggga gtgtgttgtt cattcccctg ttttatcacg ggttatatcc
     5761 ggatgccagg ccgttctgtc cgtttgggat gttgccatgc gatcccctcc agtagcatgg
     5821 ataactgagc tggcgtcagg tgcactttcc cttcccgggt taccggccag acgaagcggc
     5881 cccgttccag gcgtttggcg aacaggcata acccgtcacg atcggcccac agtattttca
     5941 ccattttgcc actgcggccc cggaagacga agatatgccc ggagaacggg gcatctttca
     6001 gcgtgttctg caccttcgaa gccaggccgt tgaagccaca acgcatatct gtgatgccag
     6061 cgatgatcca gattctggta ccggttggca gcgttatcat cgggtacctc ctttcatttc
     6121 gcggattagc gcccgtaaca tttccggagt gagagggtca aacagtttta ccacacctga
     6181 tttaagatgc agctcgcacc gtgggacgtt tccgggatca ctctcagggc actcatcagg
     6241 cttgttacgc cagaagggat ttgtaactgg tctggtcggc tctggcgtat cagtcagagc
     6301 caccgggaca ggcatgcatt cctgtatgtc atcatcgctc agtaagccgt cctcgtactg
     6361 gcttttccat tttaacagca ggttatcatt gataccgtgc tctctggcga tccgggcaac
     6421 aacagcaccg ggctgtaatg cctgttttac cagcctgatt ttaaattcac gactgtagga
     6481 ggttcgtcgt tcttttcgcc atgtgccttc gctgatttga ggctctgtta attccttctt
     6541 tctgttggca taaaggatgg cgtcaagctg agctaatgaa actgaatcgg gcaatggcca
     6601 tgcgataccg gatgcaataa atcgctgaaa aagcgtatgt attgtggaat gactgagacc
     6661 tagacgctga gcgatggccc ggatggtcag tttatcttca aatcttaaac gcagagcatc
     6721 aggcaaataa gaacggaagc agggaatatc tttttttgtc tgggaattca tcgttcgtgt
     6781 ccatctatat agatgggcgc gattgttgcc agacaggaca attttcacaa gacgtcgcag
     6841 atggggcgct tacacaacaa tggcgcgagg gaaagctgc
//
LOCUS       DBFIFM010000217         6857 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.217, whole genome shotgun sequence.
ACCESSION   DBFIFM010000217 DBFIFM010000000
VERSION     DBFIFM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.217"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..384
                     /locus_tag="N7C74_004050"
     CDS             <1..384
                     /locus_tag="N7C74_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU2433817.1"
                     /translation="LAEFVALISESGANPFGLTVDAVMEEYRRWRNESWRYDGSDKYP
                     WPQPVLYHICLEMRSKGIERQMTEGELKRLVERQLTKWAKHVGNGLSVPPVRRQLAAP
                     KRPPGPTPIELLKQEYERRKAAGFV"
     gene            419..1117
                     /locus_tag="N7C74_004051"
     CDS             419..1117
                     /locus_tag="N7C74_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU2433818.1"
                     /translation="METVFDALKALKKASSHEIAARLEISRDDAVTELWKLKRRGEAD
                     NKGSMWWLTSEATEAAPKTTAEMLINAIEQHGPQSADELALMFGITSRRANSSLAMAV
                     SKGRLIRVNQDGKYRYCIPGDNLPAEPKAASVTETDGKAFPQPAGVALPVREAETQEE
                     IKTESVAVTVQSQSSFIRKHPDGLILPSLHVANRELRRAKGQVQKWERLCAALREINK
                     HRDVIQKITTGDNG"
     gene            1139..1363
                     /locus_tag="N7C74_004052"
     CDS             1139..1363
                     /locus_tag="N7C74_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433819.1"
                     /translation="MKIRYHDFGPVSHMLISSTVLETRKHNHILDMLRLADPYLVINT
                     SGIFFLRSTVSGKTSHVLRAYKTAVREEGE"
     gene            1360..1716
                     /locus_tag="N7C74_004053"
     CDS             1360..1716
                     /locus_tag="N7C74_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eae-like protein"
                     /protein_id="HGU2433820.1"
                     /translation="MSEINYQELRVELGAAKKRIEELEANRVVLEVENERLKHAMAVA
                     LEHISVTDAGQAGVAAMIIYDAMYHSEKTDPYAFLAELRAQGVEMVREHPAIKLCSLT
                     HICDELVAELRKGGNK"
     gene            1749..1901
                     /locus_tag="N7C74_004054"
     CDS             1749..1901
                     /locus_tag="N7C74_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433821.1"
                     /translation="MAKKHHYFAEGEITSICGGWMYFGNEREPDTFESPDDCKKCRRK
                     LNKGGK"
     gene            1898..2209
                     /locus_tag="N7C74_004055"
     CDS             1898..2209
                     /locus_tag="N7C74_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433822.1"
                     /translation="MSVIKEMPVERNEYGCWTHPEYEKFCDGREYISTEEFNAWMEKN
                     NLQWAIRSMDEDDFNLDADGPDISAWEPERPEGEGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            2336..2899
                     /locus_tag="N7C74_004056"
     CDS             2336..2899
                     /locus_tag="N7C74_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433823.1"
                     /translation="MTELTKEWLQNTITGIESSRDEIPFGLDEDQNNMLTALKIALAS
                     LASVSDERAAYELFMEKRFGESVDRRRAKNGDREYMAWDMALGWIIWCHRAAMLQAGN
                     FRENKGSSTNNFRIISETSTNSPAIPDEVLSAILKVARLRADFDASEVDRRGIGSCLD
                     EAEQELIVTINEYASQIAVEATQGENQ"
     gene            3009..3113
                     /locus_tag="N7C74_004057"
     CDS             3009..3113
                     /locus_tag="N7C74_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433824.1"
                     /translation="MSDDISLVMEGALAVIAVVGVYCLVVFLMERLGN"
     gene            3357..3512
                     /gene="hokD"
                     /locus_tag="N7C74_004058"
     CDS             3357..3512
                     /gene="hokD"
                     /locus_tag="N7C74_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGU2433825.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            3680..3958
                     /locus_tag="N7C74_004059"
     CDS             3680..3958
                     /locus_tag="N7C74_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433826.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            3960..5009
                     /locus_tag="N7C74_004060"
     CDS             3960..5009
                     /locus_tag="N7C74_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU2433827.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWAACNQVVDALPEAVARRSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMRRPKRCRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLEFEKKYGSQIEL
                     IFRFLDHAFATGVLG"
     gene            5022..5381
                     /locus_tag="N7C74_004061"
     CDS             5022..5381
                     /locus_tag="N7C74_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU2433828.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGQ
                     LVPGERLGIKITELECA"
     gene            5378..6067
                     /locus_tag="N7C74_004062"
     CDS             5378..6067
                     /locus_tag="N7C74_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HGU2433829.1"
                     /translation="MNNHYLQFVRELLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKVTSIPLVSPVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNTQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYKELALLVGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKQKA
                     AFSKQSVAKVD"
     gene            6278..6353
                     /locus_tag="N7C74_004063"
     tRNA            6278..6353
                     /locus_tag="N7C74_004063"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6311..6313,aa:Ile,seq:cat)
     gene            6361..6437
                     /locus_tag="N7C74_004064"
     tRNA            6361..6437
                     /locus_tag="N7C74_004064"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6395..6397,aa:Arg,seq:tcg)
     gene            6451..6527
                     /locus_tag="N7C74_004065"
     tRNA            6451..6527
                     /locus_tag="N7C74_004065"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6485..6487,aa:Arg,seq:tct)
     gene            6701..>6857
                     /locus_tag="N7C74_004066"
     CDS             6701..>6857
                     /locus_tag="N7C74_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HGU2433830.1"
                     /translation="MVFLVKKARRAATEIKKFEKRDLAQVVINAAYLVACADGECEAS
                     EKAKIEQV"
BASE COUNT         1830 a         1536 c         1904 g         1587 t
ORIGIN      
        1 ctggctgagt ttgtggcgtt gatttcggag agcggggcaa atccatttgg cctgacggtg
       61 gatgccgtga tggaggagta ccgtcgctgg aggaatgaat cctggcggta cgacgggagt
      121 gataaatacc cgtggccaca gccggtgctg taccacattt gcctcgagat gcgttcaaag
      181 gggattgagc gccagatgac cgaaggggag ttaaaacggc ttgtggaaag gcagctgacg
      241 aaatgggcaa agcatgttgg taatggcctc agtgttccgc cagtccggcg acaactggca
      301 gcacccaaac gcccgccagg gccaacgcca attgaattgc tgaaacagga atatgagcgc
      361 cggaaggcgg ccggttttgt ctgagttgag aagtaatttt taccgggagg aaaatttaat
      421 ggaaaccgtt tttgacgcac tgaaagcact gaaaaaagcc tcgtcgcacg agattgcagc
      481 ccgtcttgaa atcagccgtg acgatgctgt taccgaactc tggaagctga agcgtcgtgg
      541 cgaagctgat aacaaggggt cgatgtggtg gctgacgagt gaggcaactg aagcggcccc
      601 aaaaaccact gcagagatgc tgattaacgc gattgaacag catggtcctc agtcggctga
      661 cgaactggcg ttaatgttcg ggattacctc ccgccgggcg aattcatcac tggcgatggc
      721 tgtcagcaaa ggtcgtctga ttcgcgtaaa tcaggacggt aaatatcgtt actgcatacc
      781 gggcgataat ttaccggcag agccgaaagc tgcatcggta acggaaaccg atggtaaagc
      841 ctttcctcag ccggcaggtg ttgcgttacc agtccgggaa gcggaaacac aggaagaaat
      901 taaaactgaa agtgtggcgg tcacagtgca gtcacagtcg tcgttcatca gaaagcatcc
      961 ggatggtctg attttaccat cgctgcatgt ggctaaccgc gagctgcgcc gggcaaaagg
     1021 tcaggttcag aagtgggagc gcttatgcgc cgcgctgcgg gaaattaata agcatcgtga
     1081 tgtcatacag aaaataacga caggagataa cgggtaattt ttacagcagg gtgatgatat
     1141 gaaaatcaga tatcatgatt tcggccccgt gtcacatatg cttatttcaa gtacggtgct
     1201 ggaaacaaga aaacataatc acatactgga tatgctgcgt cttgctgacc cgtatctggt
     1261 aataaacacc agtggaattt tctttctgag aagcactgtg tcaggaaaaa catcgcatgt
     1321 gcttcgggca tataaaacag cagtgcggga ggaaggagaa tgagcgagat taactatcag
     1381 gagctgcgtg ttgagctggg agcagcaaaa aaacgcatag aagagctgga ggctaatcgt
     1441 gtggtgcttg aggtggaaaa tgagaggttg aaacacgcaa tggccgtagc ccttgagcat
     1501 atatccgtca cagatgcagg acaggcaggt gtcgctgcaa tgattatcta tgatgccatg
     1561 taccacagtg aaaaaactga cccctacgct tttctggcag aattgcgggc gcagggtgtg
     1621 gagatggtgc gcgaacatcc agcaatcaaa ctttgctctt tgacgcacat atgtgacgag
     1681 ttagtcgcag agcttcgcaa aggaggcaac aagtgagtaa aacgaatccg ggctgggcaa
     1741 ggtcacttat ggcgaaaaaa catcattatt ttgctgaagg tgaaataaca agtatttgcg
     1801 gtgggtggat gtattttggc aatgagcgtg agccggatac attcgaaagt ccagatgatt
     1861 gtaaaaagtg tcgtagaaaa ttaaataagg ggggtaaatg agcgtaatta aagaaatgcc
     1921 agtggaacgt aatgaatatg gctgctggac gcatcctgaa tatgaaaaat tctgtgatgg
     1981 tcgggaatat atttcaacgg aagagtttaa cgcctggatg gagaaaaata atcttcagtg
     2041 ggctatccgt agtatggatg aagatgattt taatctggac gcagatggcc ctgatatttc
     2101 agcatgggaa cctgagcgac cagaaggtga gggatggttt atcggttcga tacatgacac
     2161 cgaagatggt cctgtttgtg tatggctgag aaataaggcc gaagcataaa ggctataaac
     2221 caacaactaa acactgaaaa tttaaatcag aaatgatttt tattaaatcc ttaaccggag
     2281 ggattcctgc accctcagaa catcaggaga ccgcccgaaa gggcggtagt gaaaaatgac
     2341 tgaattaacc aaagaatggc tacagaacac gattaccgga attgagtcat cacgggatga
     2401 aataccgttc ggactcgatg aagatcaaaa caacatgctt accgcattaa aaattgcact
     2461 ggcatcactg gcatcagtat cggatgaacg ggcagcctat gaattattta tggagaagcg
     2521 tttcggggaa tctgtagatc gccgcagagc aaaaaatggc gatagagaat acatggcatg
     2581 ggatatggcg cttggctgga ttatctggtg tcaccgcgcc gccatgcttc aggctggaaa
     2641 ctttcgggaa aataagggtt cgtcaaccaa taattttcgg ataatctcgg aaacgtcaac
     2701 caactctccg gcaatccctg atgaggtgtt gtccgcaatc ctgaaggtcg ccaggcttcg
     2761 tgcagatttc gatgcttctg aagtggacag gcgaggtatc ggtagttgtc tggatgaggc
     2821 cgagcaagaa cttatcgtta ccattaacga atacgccagt cagatcgcag tagaagcgac
     2881 acagggggag aaccaatgag ccggccagat gcatttgcag gcgttgctat agctattgct
     2941 tttctagtat atgttatttg tcggtggggg taaaaacgtt cgccgggatt cacaccaagg
     3001 gagggaatat gtcggatgat atttcactgg taatggaagg cgctctggct gttattgctg
     3061 ttgtgggtgt ttactgcctg gttgtgtttt tgatggagcg cctggggaac tgaattcatt
     3121 ccgtatggga attcccatat cgggcaaaac ggtttgctgt aaagcgagag ttaagtagaa
     3181 ttgctgcggg tgcttgaggc tgtctgcctc gggcatgcca ccgtaaggca gacagagaaa
     3241 agccccagtt aacattacgc gtcctgcaag acgcttaaca ttaatctgag gcccaatcta
     3301 tgcttcacaa acgtaggtta gcctcttacg cgccgaaagg caaggagaag caggttatga
     3361 agcagcaaaa ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg
     3421 cactggtaac gaggaaagac ctctgcgagg tacgaatccg aaccggccag acggaggtcg
     3481 ctgtcttcac agcttacgaa cctgaggagt aagagaccag gcgggggaga aatccctcgc
     3541 caccgctgat gtgtcaggca tcctcaacgc acccgcactt aacccgcttc ggcgggtttt
     3601 gttttttctg gtcgttctgg tttacaatcc atccgtcagc ctgaacaact ggcacctgct
     3661 gcgccagcag agaaaacaga tggcgcacga taccaaattt tacaattcgg ataactctgc
     3721 cgcccctgcc agcaggcacg ggcggcgttc tcatgcattc aaatctgact ggtatcagca
     3781 cgacccctgc accgaagaac aggctgaatg gctgattcag tgttaccgca ggcgcggata
     3841 cgaggttaag aaagccctca gtctcgatta tcgtcactgg ataatctccg tcaggcttcc
     3901 ttactccgaa cgcccaccgc gtccgtcccg cacattccag caacgcatct ggaggtaacg
     3961 tgcgggtatt acttcgacct gttctggtac cggaactcgg gctggtgatc gttaagccgg
     4021 gccgtgaatc catgccggta ttccacaata cccgggtact ggtggagccg gaaccgaaaa
     4081 gcatgcgtaa tctgccgtcc ggggtcgttc ctgccgttcg ccagccgctg gcggaggata
     4141 aatcattact gccatttttc agcgacgaac gagtgattcg tgctgctggt ggcgctggcg
     4201 cactgtctga ctggttactg cgccatgtta aatcctgcca gtggccacac ggcgattatc
     4261 accacagtga aaccgtcatt caccgttatg gtaccggcgc aatggtgttg tgctggcact
     4321 gcgacaacca gttgcgtgac cagacctccg aatcacttga gcaacttgct caccaaaacc
     4381 tgtcagcatg gatgattgac gtcattcgtc acgcaatgaa tggcacacag gagcgtgaat
     4441 tatcgctggc tgaattatcc tggtgggcgg cctgcaatca ggtggtggat gcactacctg
     4501 aggcagtagc gcgtcgttcg ctgggattac cagcggaaaa aatccgctcc gtataccgtg
     4561 agagtgacat cgtaccggga gaacagacag ccatcagcat actgaagcag cgcacaaaaa
     4621 atattgcgct gccacttcac gtccaccagc aacaaaatcc accacagaaa aaaacggttg
     4681 tcagtatcgc cgttgatccg gagtctcctg aatcgttcat gaggcggcct aaacgttgcc
     4741 gctgggttaa tgagaaatac acgcgctggg taaagacaca gccgtgtgcg tgttgtggta
     4801 agcctgctga cgatccgcat cacctgattg gtcatggtca ggggggaatg gggacaaagg
     4861 cccacgatat tttcacgcta ccgttgtgcc gggagcacca caacgaactt catgcagacc
     4921 cgctggagtt tgagaaaaag tacggctctc agattgagtt aatttttcgt tttcttgatc
     4981 acgcctttgc gactggcgtg ctcgggtaaa agaggtgact gatgctcata gatttggttt
     5041 taccttaccc gccgacggtg aacacctact ggcgacgtcg tggcagcaca tattttgtat
     5101 caaaagccgg tgagcgttat cgccgggctg tggcgcttat tgttcgccag cagcggctga
     5161 aattaagcct gtccggaagg ctggcgataa agattattgc cgagccaccg gataagcgcc
     5221 gccgtgacct ggacaatatt ctgaaagcgc cgctggatgc gctgacgcat gcggggttgc
     5281 taatggacga tgagcagttt gatgaaatca atattgtgcg cggtcagctc gttcctggtg
     5341 agcggctggg gataaaaatc acagaactgg agtgcgcatg aataaccact atttacagtt
     5401 tgtgcgtgag ctgctcatta tcgctaccgc cgatttgagt ggggcaacaa aaggtcagct
     5461 tgaagcctgg caggagaatg ccatgttcga tacagggcgt tacaggcgaa aaaaaatccg
     5521 gtaccgcgat gaagtgactg gaaaaatgat aacgcgggat aatccaccaa tcccgggaaa
     5581 gcaatcgctg gcgaaggtga cgtcaattcc tctggtcagt ccggttgagt tttcgacatc
     5641 atcgtggcgg cgggctgttc tgtctcttga agaacatcat aaagcctggt tgttgtggtg
     5701 ttacagcggg agtatttgtt gggaatatca gatcgcgata acacagtggg cgtggaatga
     5761 atttaatact caatccggta ccagaaaaat tgcagggaaa acgcaggaac gcctgaaaaa
     5821 attaatctgg ctggcggcgc aggcagtaaa agcagaactt tttggtgggg aaggttatga
     5881 atacaaggag ctggcattac tggtgggagt gacaactaaa aactggtcca aaacatttac
     5941 tcgtcactgg gttgcaatga aacacatttt tcaccgactg gatagtgagg ctttattgtt
     6001 tgtaatgaga acgcgttcaa aacaaaaggc ggcattttca aagcaaagtg ttgcaaaagt
     6061 agattgaaag gcatatattt catgcaaatc tgatattttg ccgattttgt acgtgatggc
     6121 aaaagcaaac aaaacccgcc cacaagcggg tttttttgtg ccacttatct cggatagaca
     6181 tggtgaatgc gctggtggag gaagcaaggg taatttttaa ccaggtgatt cttgaatgct
     6241 tgcaacattg atttcgtaac gttattatcc tgcgcccggc cctttagctc agtggtgaga
     6301 gcgagcgact cataatcgcc aggccgctgg ttcaaatcca gcaagggcca ccatcacata
     6361 ccgccattag ctcatcggga tagaacgcca gccttcgaag ctggtttcgc ggggttcgag
     6421 tctccgatgg cggtccatta tcggtattct gcgtttttag ctcagccgga cagagcaatt
     6481 gccttctaag caatcggtca ctggttcgaa cccagtacaa cgcaccacac ttattttccc
     6541 tcgctcgctt ttgcggacct tttttgtatc cgcaccacgc ccggcgcata ccaaccacag
     6601 agcctttcgg ggggagctta tggagtggtc agtgtgactt tctctgtggg cagatcgctc
     6661 ccgggcgttg gctcacccac ccaaaggaac gtcacgatgt ttggtatttt tggtaaaaaa
     6721 agcccgcaga gcggcaacgg aaattaaaaa gtttgaaaaa cgcgatctgg cacaggtggt
     6781 gattaacgcc gcatacctgg tggcctgtgc agatggtgaa tgtgaggcat ccgagaaagc
     6841 gaagatcgaa caggtac
//
LOCUS       DBFIFM010000218         6841 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.218, whole genome shotgun sequence.
ACCESSION   DBFIFM010000218 DBFIFM010000000
VERSION     DBFIFM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.218"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(37..1173)
                     /locus_tag="N7C74_004067"
     CDS             complement(37..1173)
                     /locus_tag="N7C74_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU2433831.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1176..1538)
                     /locus_tag="N7C74_004068"
     CDS             complement(1176..1538)
                     /locus_tag="N7C74_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HGU2433832.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2075..3988
                     /gene="mtlA"
                     /locus_tag="N7C74_004069"
     CDS             2075..3988
                     /gene="mtlA"
                     /locus_tag="N7C74_004069"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HGU2433833.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVETLSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4083..5231
                     /gene="mtlD"
                     /locus_tag="N7C74_004070"
     CDS             4083..5231
                     /gene="mtlD"
                     /locus_tag="N7C74_004070"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGU2433834.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            5231..5818
                     /gene="mtlR"
                     /locus_tag="N7C74_004071"
     CDS             5231..5818
                     /gene="mtlR"
                     /locus_tag="N7C74_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HGU2433835.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(5830..6039)
                     /gene="yibT"
                     /locus_tag="N7C74_004072"
     CDS             complement(5830..6039)
                     /gene="yibT"
                     /locus_tag="N7C74_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HGU2433836.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6324..6686
                     /locus_tag="N7C74_004073"
     CDS             6324..6686
                     /locus_tag="N7C74_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HGU2433837.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1711 a         1730 c         1757 g         1643 t
ORIGIN      
        1 acaaggagca aagttccata atctcccccc tccccctcaa tgatccaaat aaagataatg
       61 catccagctg gtcattctta gcagcacttt ccgcatcacc gaaacatggc tgaaatggtc
      121 ggagtaaacc gccacctggg cgtagatgcc gtcgggtaag gcatcgatat catcgttagg
      181 gtccagttca atggttccca gcacaccgtc cgtgccgggc acgaccgtta atgattgcaa
      241 taccccctgc gcctgataag aaccgcctgg cacgacaggt aaaatactag tcagtttgcc
      301 gtgaaacacc tgcccaggta gcgcgttaaa caccacttcc gcatcatcgc cgggtttcag
      361 acgtaacagc gagttttgcc gaaattgggc gacaatttgc cgtttttgct cggggatgaa
      421 gaccatcacc ggacgcagcg gcaaggcagc tgcgtatgta cctgggcgga tcagtacctg
      481 agtaacgtag ccattgctcg gcgcgcgaat gacagtctgc tcaaggttat attttgcttc
      541 agtaagttgc gctcttaagc tcacaatctg cgattgctcg ccgttaacca tactgtcgag
      601 ctggctctgg atctgcgcct gctccgccac cgagcctttc accagcgcat cctgcgcgag
      661 gaaattttgc cgcgcatcgt cgatgtcacg ttccgagaac ggattcaccg ccgcctggct
      721 gcctttcaag taacgttgat aatttttaaa cagacggtcg cgctccgctg aaacctgggt
      781 ggtgttggcc tgcgcttcag tgagctgcgc acgcagcgtc tttatattat gcgtcgccgt
      841 catcaggtca gcctgaagtc tgtcaactcg cgcctggtaa cgaaccgggt cgagcttaaa
      901 aagcacctcg cccttttgaa taagctgatt attcttgtca gtgacttcag taacaattcc
      961 cgtcacctgt ggcgtgatag ggatcgctat cactgccttt tgcgcggtaa aagtgtaagg
     1021 gtggttgtag ttcatcaata aaatcaaacc actcaccaga aacacgcctc ccagcgccgc
     1081 cgtcgccagc gtccactgat ttaccggaat gcggaagatt ttaaagaccg cccacgccag
     1141 cgccacgtaa gttaaaacaa tcaatagatc cataattaga tctccgggaa cgtggaatag
     1201 tcagtctttt tctcggcggc gagttgatgc tccagccggg aaattcggtc agaaagtgag
     1261 gcgatttccg ggtcagtcgc tttctcctgc gacgcaacat gtgactgcat accccagcca
     1321 cgctccggtt gatagagcgt cgcccagatc cacagaaacg gccagataac atgcagagta
     1381 aacaggctca cccagcccgc cgtatgaata gcgtcggcat gggggtgatt gcgctttttg
     1441 gcaatcagat aagggatgtc atgtatcgcg atgattccat aaaaaatcac cagaaagacg
     1501 aagatcagca ctcccaacgc gaaatagttt agaaacatat ctgcctcgga ttcacgttta
     1561 tcagtgttgt ttttgggctg gcagccagaa gggagtcagg ctgatatttt gacaataatc
     1621 cgggttcgcg attctcgcca taacaccaaa gaataatttt tagaggtgat gagttgctta
     1681 gttacataac gattgtatga cgaaggcata acatgctgta gatcacatca ggtgaacgcc
     1741 gtaagaaaat atcttgtgat tcagatcaca aagattcaac aaaccatcaa aacaaaaatg
     1801 tgacactact cacatttaaa tgccattttt agcgaaaatc gccgccttgt tgctttttta
     1861 cacaagcgtt ttgtgatgaa cgtcacgtca attacctctc taccccctat atttatgtga
     1921 ttgatatcac acaaaaggcc gtcgactgga cagttaaccg attcagtgcc agatttcgca
     1981 gtatctacaa ggtccggcta cctctgccgc cacattaaca aaaaacctcg ggcttccagc
     2041 ctgcgcgaca gcaaacataa gaaggggtgt ttttatgtca tccgatatta agatcaaagt
     2101 gcaaagcttt ggtcgtttcc tcagcaacat ggtgatgcca aatatcggcg cgtttatcgc
     2161 gtggggtatc atcaccgcgt tatttattcc aacagggtgg ttaccgaacg agacgctggc
     2221 gaagctggtc gggccgatga tcacttatct cctgccgctg ctgatcggtt ataccggtgg
     2281 taagctggta ggcggcgaac gtggcggcgt agtcggtgcc atcaccacca tgggcgttat
     2341 cgtcggcgca gacatgccga tgttcctcgg ttctatgatt gcaggtccgc tgggcggctg
     2401 gtgcattaag cacttcgacc gctgggtaga cggtaagatc aaatccggtt ttgagatgct
     2461 ggtgaataac ttctccgcag gcatcatcgg gatgatcctc gctattctgg cattcctcgg
     2521 cattggcccg attgttgaaa ccctgtccaa aatgctggct gcgggcgtta acttcatggt
     2581 tgtccatgac atgctgccgc tggcgtctat ctttgttgaa ccggcgaaaa tcctgttcct
     2641 caacaacgcc attaaccacg gtatcttctc gccgctgggt attcagcagt cccatgaact
     2701 gggtaaatca atcttcttcc tgattgaagc taacccaggt ccaggtatgg gcgtgctgct
     2761 ggcgtacatg ttctttggtc gtggtagcgc taaacagtct gcgggcggtg cggcaatcat
     2821 ccacttcctg ggtggtatcc acgaaatcta cttcccgtat gtgctgatga atccgcgtct
     2881 gatcctcgca gtcatcctcg gcggtatgac tggcgtgttc acgctgacta tcctgggcgg
     2941 tggtctggtt tctccggcat ctccgggttc tatccttgct gtactggcga tgacaccaaa
     3001 aggtgcttat ttcgctaaca tcgcgggtgt gtgtgcggcg atggctgtct ccttcgttgt
     3061 ctctgctatt ttgctgaaaa ccagcaaagt gaaagaagaa gatgatattg aagcagcaac
     3121 tcgtcgtatg caggacatga aagctgagtc taaaggtgca tctccgctgt ctgcgggcga
     3181 tgtgactaac gacctgagcc acgtacgtaa aatcatcgtt gcctgtgacg ccggtatggg
     3241 ttccagtgcg atgggcgcag gcgtgctgcg taagaaaatt caggatgcag gtctgtcaca
     3301 gatttcggtc actaacagcg cgatcaacaa cctgccgcca gatgtggacc tcgtcatcac
     3361 tcaccgtgac ctgaccgaac gcgctatgcg ccaggttcct caggcacagc atatttcgct
     3421 aaccaacttc ctcgacagcg gcctgtacac cagcctgact gaacgtctgg ttgctgccca
     3481 gcgacatact gaaaacgaag tgaaagtgaa agacagtctg aaagacagct ttgacgattc
     3541 cagtgctaac ctgttcaaac tgggcgcgga gaacatcttc ctcggtcgca aagcagcaac
     3601 caaagaagaa gcgattcgtt ttgctggcga gcagctggtg aaaggcggtt acgttgagcc
     3661 ggagtacgtt caggcgatgc tggatcgtga aaaactgacc ccgacttatc tgggtgagtc
     3721 tatcgcggtg ccacacggta cggttgaagc gaaagatcgc gtactgaaaa cgggcgtggt
     3781 gttctgccag tacccggaag gcgtgcgctt cggtgaagaa gaagatgaca ttgctcgtct
     3841 ggtgattggt attgctgccc gtaacaacga gcacattcag gttatcacca gcctgaccaa
     3901 cgcactggat gatgagtccg tcatcgagcg tctggcacac accaccagcg tggatgaagt
     3961 gctggaactg ctggcaggtc gtaagtaatc caatcctacc ctctcctcac ggagagggtt
     4021 tgggtgaggg aaaagcctca ccccagccct ctcgggtaaa aacattgatg aaggttaata
     4081 ctatgaaagc attacatttt ggcgcaggta atatcggtcg tggctttatc ggtaaactgc
     4141 tggcagacgc gggtatccaa ctgacgtttg ccgatgtcaa tcaggtggta cttgatgccc
     4201 tgaatgcccg tcatagctat caggttcatg tggtcggtga aaccgagcag gtagataccg
     4261 tttccggcgt cgatgctgtc agcagcattg gtgatgatgt cgttgatctg attgctcagg
     4321 tcgatttagt cactaccgcc gttggcccgg ttgtgctgga acgtattgct ccggctatcg
     4381 ccaaagggct ggtgaaacgt aaagaacaag gtaatgaatc cccgctgaac atcatcgcct
     4441 gtgaaaacat ggtacgcggt accacgcagc tgaaaggtca tgtgatgaac gccctaccag
     4501 aagacgccaa agcgtgggta gaagaacacg ttggctttgt cgattccgcc gttgaccgca
     4561 tcgtaccgcc ttcggcttcg gcaactaacg atccgctgga agtgacggta gaaactttca
     4621 gcgaatggat tgtcgataaa acgcagttca aaggcgcact gccgaacatc ccaggcatgg
     4681 agttaaccga caacctgatg gcatttgtcg aacgtaaact cttcaccctg aacacgggtc
     4741 atgctataac cgcgtacctc ggaaaactgg ccggtcatca gaccattcgt gacgctattc
     4801 tcgacgagaa aatccgcgcg gtggtaaaag gtgcgatgga agaaagtggt gcggtactga
     4861 tcaagcgcta cggctttgac gcagacaaac atgcggcgta catccagaaa atcctcggtc
     4921 gttttgagaa cccgtatctg aaagatgatg tagagcgcgt aggccgtcag ccgctgcgta
     4981 aactgagtgc tggcgaccgt ctgatcaagc cattgctcgg tacgctggaa tacagccttc
     5041 cgcacaagaa tctgattcag gggattgctg gtgcaatgca cttccgcagt gaagacgatc
     5101 cgcaggctca ggaactggca gcactgatcg ctgacaaagg tccgcaggcg gcgctggcac
     5161 agatttccga tcttgatgcc aacagcgagg ttgtatccga ggcggtaacc gcttataaag
     5221 caatgcaata atggtggacc aggcgcagga caccctgcgc ccgaataaca gattgtcaga
     5281 tatgcaggca acaatggaac aaacccaggc ctttgaaaac cgtgtgcttg agcgtctgaa
     5341 tgctggcaaa accgtgcgaa gctttctgat caccgccgtc gagcttttga ccgaggcggt
     5401 aaatcttctg gtgcttcagg tattccgcaa agacgattac gcggtgaagt atgctgtaga
     5461 accgttactc gacggcgacg gtccgctggg cgatctttct gtgcgtttaa aactcattta
     5521 cgggttgggc gtcattaacc gccaggaata cgaagatgcg gagctgctga tggcattgcg
     5581 tgaagaacta aatcacgacg gcaacgagta cgcctttacc gacgacgaga tccttggacc
     5641 ctttggggaa ctgcactgcg tggcggcgtt accaccgccg ccacagtttg aaccagcaga
     5701 ctccagtttg tatgcaatgc aaattcagcg ctatcaacag gctgtgcgat caacaatggt
     5761 cctttcactg actgagctga tttccagaat cagcttaaaa aaagcctttc aaaagtaagc
     5821 aacgtctgct tactgcccct ctacctgctt cggccgataa agccgacgat aatactccag
     5881 acgttgtaga tataacggca cgctttcatc gggtattccg gacggaatcg cgttacgggg
     5941 aggaagtttt ttcagatact cccggaacgc ctggcttgat gccatgaaat ctacggcttt
     6001 atcgataaga agcggaacgt tttcacctaa tttgcccatg atgatatctc cgtattaccc
     6061 cgcgccggga atgcgcggcc gccaatttta gtttaggttc ccggtaaaga tgacattaag
     6121 gaaaacgtgc tgaatcctca ataatcatcc gctgctgttt attatctttt ttctatagat
     6181 ttcttaatta atcaataaat tgcgtagttt ttaagcatgt taatgattac gttacagaac
     6241 tattgcaatg tcatcagttg tgaacaaagc acctggtcgc gcatactagg ggctataaat
     6301 ttatctttat cagaagccat cacatgaaag aagtcgaaaa aaacgaaatc aaacgtctca
     6361 gcgatcgtct ggacgccatc cgccaccagc aggccgatct gtcgctggtt gaagccgcag
     6421 acaaatatgc cgagctggaa aaagagaaag ccacgctgga agcagaaatt gctcgcctgc
     6481 gtgaagttca tagccaaaaa ctgagtaaag aagcacagaa gctgatgaag atgccgtttc
     6541 agcgcgcaat taccaaaaaa gagcaggctg atatgggcaa gctgaagaaa agtgttcgcg
     6601 gactggtggt tgtgcaccca atgaccgcgc tgggccgtga aatgggcctg caggagatga
     6661 ctggattctc taagactgcg ttttaagcgc ctcagacgac tggttcgatc atatcaaccc
     6721 gcatctggct tgccttagct ggtcaatgcg gggactctcc tgtctactcg cctaattacc
     6781 aaacaattca ctccctttac gtcgatttac cgctggttga tattctgaaa atgcattttt
     6841 a
//
LOCUS       DBFIFM010000219         6838 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.219, whole genome shotgun sequence.
ACCESSION   DBFIFM010000219 DBFIFM010000000
VERSION     DBFIFM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.219"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            2..280
                     /locus_tag="N7C74_004074"
     CDS             2..280
                     /locus_tag="N7C74_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HGU2433838.1"
                     /translation="MVIFYGIIILHDIPYLIAKKRNHPHADAIHVAGWVSLFTLHVIW
                     PFLWIWATLYRPERGWGMQSHDSSVMQLQQRIAGLEKQLADIKSSSAE"
     gene            286..1422
                     /locus_tag="N7C74_004075"
     CDS             286..1422
                     /locus_tag="N7C74_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU2433839.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQKAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELNEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1419..2393)
                     /locus_tag="N7C74_004076"
     CDS             complement(1419..2393)
                     /locus_tag="N7C74_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2433840.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTLFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            2517..3257
                     /locus_tag="N7C74_004077"
     CDS             2517..3257
                     /locus_tag="N7C74_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HGU2433841.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(3387..5357)
                     /locus_tag="N7C74_004078"
     CDS             complement(3387..5357)
                     /locus_tag="N7C74_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HGU2433842.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLG
                     VVCRLADHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEKADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     TQTLTFIPWFSWANRGEGEMRIWVNEEKHCHP"
     gene            complement(5368..6768)
                     /locus_tag="N7C74_004079"
     CDS             complement(5368..6768)
                     /locus_tag="N7C74_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2433843.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCARINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVALFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTHVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
BASE COUNT         1648 a         1715 c         1798 g         1677 t
ORIGIN      
        1 tctggtgata ttctatggga ttattatttt acatgatatt ccctacctga ttgctaaaaa
       61 acgtaatcat cctcatgccg acgccattca tgttgctggt tgggtgagtc tttttacgct
      121 gcatgttatc tggccatttt tgtggatttg ggccacgctt taccgcccgg agcggggatg
      181 gggaatgcaa agccatgatt catccgttat gcaactgcaa cagcgcattg ccgggctgga
      241 gaaacagctc gccgacatca aatcctcttc tgccgagtaa tacttatgga tttactgatt
      301 attttgacct atgtggcttt tgcatgggca atgtttaaga tcttcaaaat tcccgtaaat
      361 aaatggacca ttcccacagc ggccctgggt ggaatattta ttgtcagcgg tctaattctg
      421 ttaatgaact ataaccatcc gtataccttt aaagcgcaaa aagcggttat ttctattcct
      481 gttgtcccac aggtgacagg cgtggtgata gaagtgacgg ataagaaaaa tacgctgatt
      541 aaaaaaggtg aggtgctatt tcgactggac ccgacgcgtt atcaggcgcg ggtggatcgg
      601 ctgatggcgg atatcgttac cgcagaacat aaacagcggg cgttgggcgc agagttagat
      661 gagatggcgg cgaatactca gaaggcaaag gccacgcggg ataaattcgc taaggagtac
      721 cagcgttacg cacgcggcag ccaggcgaaa gtaaacccgt tttcagaacg cgatattgat
      781 gtggcacggc aaaattatct ggcgcaggaa gcctccgtaa agtcatcggc ggcggaacaa
      841 aaacagatcc agagccagct ggatagcctg gtgttgggtg aacattctca aatcgccagc
      901 ctgaaagcgc agctcgcgga agcaaaatat aaccttgagc agacgatagt gcgtgcgccg
      961 agcgatggtt atgtgaccca agtgctgatt cgtccgggta cctatgccgc gtcgctgccg
     1021 ctacgtccgg tgatggtgtt tatacccgat cagaaacgac aaatcgtggc gcagttccgt
     1081 cagaactcct tgctgcgcct ggctcccggc gatgatgcgg aagtggtatt taacgctctg
     1141 ccaggcaaag tgttcagcgg taagctggca gccattagtc cagctgttcc cggcggagct
     1201 tatcagtcga ccggcacctt acagacgtta aacacagcgc cgggttcaga tggcgttatc
     1261 gcgaccattg aactgaatga gcacactgat ttgagcgctt taccagacgg tatttacgct
     1321 caggtggcgg tctactctga tcatttcagc catgtctcgg tgatgcgcaa agtgttatta
     1381 cgcatgacca gctgggtgca ttacctttat ctcgatcatt aatcttcgct ttccgccgct
     1441 tgttcaggcc gcagttgctg cgactgagca agctggtggc gaatttcacg cagcatttca
     1501 tccagcacat actggacccg ccagggctgg tattcccgtt tacggaagat agccaccaga
     1561 tccagccacc attcatacgg gtgctgaaag cagggcacca gcgtgccgtc ttgcagatcg
     1621 ctctgcacac ttttgtctgg cgcaaacaca atccccaggt gatttcgtgc cagctccagc
     1681 gccgattgcg tgttatcgca aacgtaattt cccttcacgc gataatcccg cacctcttta
     1741 ctcccggcga cgttgaagcg ccagatattc gcgtcgtcga tcatcatcga gtcgatcaaa
     1801 atacaggagt gatgttcgag ttcgtcagga cggctaatag gatgtttctc aagatagcgc
     1861 tggctggcat aagcggttac ggcatattgg gtaataaaac tggcaaccag cgattcatct
     1921 tttggtgggg catagctgat taaaacatca caatcatcag gaaattcgac gccttcataa
     1981 aaggcattac gctcaagatt gcaggttttt agcgataagg tgatatcacc gatatcttta
     2041 attttatcga ttacatgttt ggataaatag gtaattatgc cggttggggc gtagatggtg
     2101 acccgaccac gtttctcatg cttataatct gcaataaaat tattaagctg ctcgtttctg
     2161 tccagcatgt cgttgatgta cggtaatagc gcggtgccaa aaagtgtgag catcagttgt
     2221 cgggtggttc ggtcaaagac ttttaaaccc acttttgatt caaaatcagc aagatatttg
     2281 ctgacgttgg cctgtgcgat gccaagtaca gtcgcggcat ggctgatatt ttcactggca
     2341 gcgattaccg agataatttt taactcccgg tacttaagtt gtaattttgt catcgcaaca
     2401 gcccaattat atataatttt atatattact atataaatat gaatcttgca ccggagaaat
     2461 tgtaagcatt actatgccga ctctttttta tcttatttta aaatggaata ccggacatgt
     2521 taattaaccg caatattgtg gcgttatttg cgttgccttt tatggcaagc gcaactgctt
     2581 ctgaattatc cattggtgct ggtgcggctt ataatgaatc gccttatcgc ggttataatg
     2641 aaaatacaaa ggcaattccg ctgattagtt atgaaggtga ttctttttat gttcgtcaga
     2701 ccacgttagg ttttattctg tcgcaaagtg aaaaaaatga acttagcctg accgcatcct
     2761 ggatgccgct ggaatttgac cctgccgata atgacgatta tgccatgcaa cagcttgata
     2821 agcgtgatag tacggctatg gcgggggttg cctggtatca ccacgagcgt tggggaaccg
     2881 tgaaagcctc tgcagctgcg gacgttctgg ataacagcaa cggctgggtg ggggagctat
     2941 cggtattcca caaaatgcag ataggtcgtc tgtcgctgac acctgcgctg ggcgttctct
     3001 attatgacga gaatttcagt gactattact atggcatttc agagagtgag tcccgtcgta
     3061 gcggtctggc aagttattcc gcgcaggatg cctgggtgcc ctatgtcagc ctgacggcaa
     3121 aatacccgat aggagagcac gtcgtattga tggcgagcgc aggatacagc gagctgccgg
     3181 aagagattac cgacagcccg atgattgatc gtaatgagag ttttaccttt gtcaccggcg
     3241 tgagctggcg tttttaattc accggtggat gtcggtgcgg ctggcctacg ctctcgcacc
     3301 gacttgtatg cctgataaga cgtgttaagc gtcgcatcag gcaatgtgct ccttacgttg
     3361 gatgcggcgc gagtgcctta tccaacctac ggatggcaat gcttttcctc attcacccag
     3421 atccgcattt cgccttcacc ccggttggcc cagctaaacc acgggataaa ggtcagagtt
     3481 tgcgtctggc gtttggctgg cgcgctgtcg taatgccaca gcggttgctg ctctggattg
     3541 ctctgttcat accggtaacc cggtgcctgg attaagatct tatggctaaa caatcccttg
     3601 ccttcaaatg tcgtaaatgg cgcatcggtg ggcagccaca gattatgcag tgactcgccg
     3661 ttgtcggcct tttccaggca atacaccagc gggccgcgct gaatcgccac ttttccggcg
     3721 acgtgacgca ccagcgggtt accgtaaacg cggcgtaccg gcatcggcaa agtcagattc
     3781 agcgtatcgc cctcctgcca ttcgcgggta atgtgcaaat accctttacg aatatcctgc
     3841 tcgacctctt ccccattcaa tatgatctgc ggctgtgtgc accagtccgg cagacgtaaa
     3901 gccagcgtat gacgtaccgg ctggggcgat tcaaccgcaa tcgtcacctg ctcctgccac
     3961 ggatagttcc cgctaacccg caggcgcagc gtgccatttt ctaccggcac ttccatgctg
     4021 tttcctgcgt agatgttgat atacaacgca tcttcacgcg gcgtgtagag ataatgacca
     4081 atcgaggtca gcacgcgggc gatatttggc ggacaacaag cgcagccaaa ccaacgctgg
     4141 cggatcggtt taacgtgatc gtagatatgg ttgaatttca gcgattttgg atgtacttcc
     4201 agtggattca catagaagaa atgtttgcca tccaatgcca tgccgccgag cacagtgttg
     4261 tacagtgcgc gctccatcac atcggcatat tgactgtcgc cttccatttc cagcattcgt
     4321 cgggcgaaca tcatcaggcc gatggaagca caactttcgg cgtaaaccgt gtcattcggc
     4381 agatcgtaat cgctgctgaa cgcttcgccg ctgctttgtg agccgatgcc gccggtaata
     4441 tataactgac gctgggccat attgttccac agccgcaggc agtcctgacg cttgctgtca
     4501 tcgtgactta aacgcgcgag atgcgcgacg ccggtcatca gatagacaaa acgtaccgcg
     4561 tgaccgatgg cggtttgctg ttgcgcaagg ggcaaatgtg cctggctgta ggctttgtct
     4621 ttcaccatcc acgccgggcc gtaggtgtgc cagtgcgatg tctgcccgcg cttttcatat
     4681 tcctggtcgt aatagtgcgg ttgcgcacca cgctgttcga caaaatagtt cgtcagcgcc
     4741 aggtagcgcg gctcttcagt cacttcatac aggcgcatta gtgccagttc aatttccggg
     4801 tgaccaggat aaccgtgtaa cttactttca tctggaccaa atacgctgtc gatatgatcg
     4861 gccaggcggc aaacgacccc cagcaagcgc cgttttcccg tggcctggaa gaaggcgact
     4921 ccggcttcaa tcagatgacc tgcgcagtaa agttcatgac actccgccag attgctccag
     4981 cgttcttcgg gtgcttttac cgtaaagtaa gtattgagat agccgtcttc gcattgggcg
     5041 gaggcgatca gttcgattac ctcgtcggcg gttttttcca gttcggcgtc cggcttctgg
     5101 cacagcgacc aggctaccgc ttccagccat ttggcgacgt cgctgtcctg aaacaccatc
     5161 ccgtaaaatt caccctcctg aagtccggcg gcaatgcgaa agttttcaat cgcatggctg
     5221 ggttccgctt ctgggatacg atcgttcaag gcatcccact gataaggaat caccacgtcg
     5281 cggaccagtt gttggtactg accgaggaac ggatcgctga ccgtcagttt atgcagatcg
     5341 acttccgaaa tgttcatctt gtgctcctta aggctgtaca tgacgctgac gcaggtcggt
     5401 agctatgcgt gacatcatag gattattgag tttgcaaaag cggatcgccc ctgccagcag
     5461 caggtggaat agcgcaggca gtaatgtttc catcgccgcg atcccgccaa gcgaactggc
     5521 ggtctggttt tccacgccag gctggtaggc gacaaaaata aacagcaggc tgatgatccc
     5581 ggcgctggag gcccaggcca gcttgatgaa aaacagattg aaggcgaagt tcatgccgga
     5641 agaacgtacg tgggttttcc actcgccgta gtcatcggca aaggccatca atgagaagtg
     5701 cagtggcaat gtgaagccaa gaatcacgcc attaccgagg atcaccgcca gccacagcgt
     5761 ttgataagcc gggccggagg gcaggaacca catcaacacc gccagtgcag cgaggagcag
     5821 gttggtgtag taataaattt tgacggtatc gaaacgccgc gttaacgggc tgacaatcac
     5881 cgagccgata atggaggcga aggtgaccat ggtgaagaac agcgacgtgt agcccgtgct
     5941 gccttgtaag acgtaggtaa tgaagtacat atacccaccg ccgcgaatgt taaagacgtt
     6001 aatcagcagg aaagacatga ccagcatcag cagcagttgg tcgttgttcc gcagcccggc
     6061 aagatgttcg cgcagggtaa atttccccat tgtggagagc ggcacccgtt cacgaaccca
     6121 gaagaaacag cacaggaaca tcaccacggc aatggcgcac aataccccga cgcccagttg
     6181 atagccgcgt gcagcgttgc cctgaccgaa gagagctacc atccacggta agcctaccga
     6241 aaccagaaat cccgctacgc cgcagagaac gaatcgccag gactggcagg agatcacttc
     6301 attgtggcgg gtggtcatgg tgttgatccg cgcgcagtac ggtacgttga tggcggtata
     6361 accgactgaa aggagcaggt aagtaccgaa cgcccaggcg atttttactc ccatgctggc
     6421 atctggcacg gtgaacgtca ggatgccgat aatgccgatg ggaatggcaa tccatagctg
     6481 ccacggacga aagcgccccc atcggctttg cgtccggtcg gcaatgacgc ccatcaccgg
     6541 gtcggagatg gcatcaaaca cgcgcagtgc aataaacagc gtgccaacca gcgccggagt
     6601 taatccaaaa acgtcggtgt agaaaaaggt gagaaaattc atgatcaggc aagttattac
     6661 ggtgccgcct gcgtcaccca ggccgtagcc tattttttcc cgtacagata acgcatcgtc
     6721 aattttacct ttagcgatat cagcggtagt aatcggtgta gaggtcatag atattcctcg
     6781 tgaataggga gcaattttta gttttattga aatgacctga ttaattctgc cgtgaacg
//
LOCUS       DBFIFM010000220         6836 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.220, whole genome shotgun sequence.
ACCESSION   DBFIFM010000220 DBFIFM010000000
VERSION     DBFIFM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.220"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..407)
                     /locus_tag="N7C74_004080"
     CDS             complement(<1..407)
                     /locus_tag="N7C74_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HGU2433844.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRCSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIA"
     gene            complement(404..754)
                     /locus_tag="N7C74_004081"
     CDS             complement(404..754)
                     /locus_tag="N7C74_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HGU2433845.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(764..1090)
                     /locus_tag="N7C74_004082"
     CDS             complement(764..1090)
                     /locus_tag="N7C74_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HGU2433846.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELIQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(1093..3672)
                     /locus_tag="N7C74_004083"
     CDS             complement(1093..3672)
                     /locus_tag="N7C74_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU2433847.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPEAVLSFHAVFACISLISQDIAKMRLRLMQTDAHGIRRETRRGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNARGQIKELRILDWSRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLIGLPPVYAAGLAATQGHHIQENSTSFFRNG
                     GRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYNPTTFSPVDAQT
                     VEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQCLQTLIESIELLLDE
                     ALETGENESTEFDVTTLLRMDSERRMKTLGDAVKNTLLTPNEARKRENLPPLAGGDAL
                     YLQQQNYSLEALSRRDAREDPFASAGKTVSSQLPDGASDGNKAISETEHDAVKAMFRG
                     DTEKMTERELSIIRALGEEFSTVLADLQRTFEGKMASQAQAFEEKLTSLSAVLQKHVT
                     VDEVRPVLQAMVDDAVGAIPVPRDGRDYDPDVLQQAVNDAVANIPQPADGKSLTPDDV
                     RPMLEQMVKEAVSHIPVPRDGRDYDPEVLQKAVNDAVANIPQPADGKSLTPDDVRPML
                     EQMVKEAVSHIPVPRDGRDYDPEVLQKAVNDAVANIPQPADGKSLTPDDVRPMLEQMV
                     KEAVSHIPVPRDGRDYDPDVLQKAVLDAVSALPAPQDGRDATALEILPAIDDQKSFPR
                     GTYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKT
                     FSLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTEDKPGEAHSSAWTLAAKRGRD
                     AGG"
     gene            complement(3618..3839)
                     /locus_tag="N7C74_004084"
     CDS             complement(3618..3839)
                     /locus_tag="N7C74_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433848.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            complement(3884..5821)
                     /locus_tag="N7C74_004085"
     CDS             complement(3884..5821)
                     /locus_tag="N7C74_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGU2433849.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPGGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGSASGG"
     gene            complement(5885..>6836)
                     /locus_tag="N7C74_004086"
     CDS             complement(5885..>6836)
                     /locus_tag="N7C74_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HGU2433850.1"
                     /translation="ARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAMVNPNL
                     GYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQQGRRV
                     SLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVVRRKSE
                     ASRFQDLVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQILDSLAE
                     AGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGDQPLMAWCVGNARVEPKGNAIL
                     ITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
BASE COUNT         1399 a         2279 c         1693 g         1465 t
ORIGIN      
        1 gcaatcgcca cctgtgccgc ctgttcactg cgcacatcaa atgccgggcg cacaaacggg
       61 tgcggtggca tattcacggt ccccatttcc acaaaccgcc agtagaaagc gttgcgcggg
      121 ttatccgcct tcatggtgtt atcgctgtta ccggtgtccg gattaacacc ccggatatgc
      181 acaccggatt ccatcccgcc atcgcgggag caccgggaaa ggaccaccac attgcggcga
      241 agttttcccc tgcgtaccgg tgcccgtgac accacttctt ctttcagcac attcgcaccc
      301 gcacgggttg cctcacgcag cacccggtta ttttccgcac cactcagaag ctgcaaatcg
      361 cggctgatgt cctccagccc cgaaaaatcc agcagggttt cgatcatttt tcccctccca
      421 gccgacagag aatttccaga cgtccgccgg tcgcatccgg cacgggcagc ccgacaacgt
      481 tcaggatccg gtcacgccag ggaccactca gcacatgaag tcgtgacgct gccgtgattt
      541 cccgaccgga ctgaccgcgc acccagatgc ggatttccgc ctgcgccatt tccgcaccgg
      601 actgcatccg ctcccggctg ctcctgcccc ggatatccgc atgaattttc ccgcatgaca
      661 cccattcttc cgtcatttct ccggcagcgt tacgggttaa caccgggttc agaacactga
      721 tcatctgtgt cagacgacct gcagatattg ccattccccc tcctcataac accgtcggac
      781 aacgcaaatc gtaaatcagc acggaaacag aaaacggcag ctccccctga atcagttctt
      841 cccgctccgc aagatccgga ttccggtaca gcatcccggt cagtcgcatg gcagccccct
      901 tcatccgggt taatgcctcg cccgggatca gttcaccgtc ctcacgaatc accttatccc
      961 ggctgccctg aatgtaggcc agcagcacgg cggtagcctg acgaaccttg tccatcagca
     1021 tgtcatcatc cgcgtcatgg tcaacacgca gatgtgcctt gatctcttcc agtgtcagta
     1081 atgccgtcat tttcagcctc ctgcatcccg cccacgtttt gcagccaggg tccaggctga
     1141 tgaatgagct tctccgggtt tatcttcggt catactgttg cagtgccaca gcgagccccc
     1201 ccacgtcacc gtatcgccgg ggtggtaggt ttcaccggct ctgaacacac cgcggtagag
     1261 catcaccggc agggaaaatg ttttttccgt acgctggcca ctgctctgcc ggaccaccac
     1321 agagaacaac cgctcacccg tcatgctgac gtcaatatcc gccaccccgt caaccaggca
     1381 ttcccatccc cgcatcccgt gcgttttttc atacgcccgc cagagtccgc cctggtgtgt
     1441 ggcatacgtg ccccggggaa aggatttttg atcgtcaatg gcggggagta tttccagtgc
     1501 cgtggcatca cgcccgtcct gcggagccgg cagggcactc accgcatcca gaaccgcctt
     1561 ctgcagaaca tccggatcgt agtcacgacc atcacgcgga acaggaatat ggcttaccgc
     1621 ctccttcacc atctgttcaa gcatcggacg cacatcatcc ggggtgagac ttttaccgtc
     1681 tgccggctgc ggaatatttg cgaccgcatc attcaccgcc ttctgcagta cttccggatc
     1741 atagtcacga ccgtcgcgcg gaacagggat atggcttacc gcctccttca ccatctgttc
     1801 aagcatcgga cgcacatcat ccggggtgag acttttaccg tccgccggct gcggaatatt
     1861 tgcgaccgca tcattcaccg ccttctgcag tacttccgga tcgtagtcac gaccatcacg
     1921 cggaacaggg atatggctta cagcctcctt caccatctgt tcaagcatcg gacgcacatc
     1981 atccggggtg agacttttac cgtccgccgg ctgcggaata tttgcgaccg catcattcac
     2041 cgcctgctgc agtacatccg gatcataatc acgaccatca cgcggtaccg gaatggcccc
     2101 cacagcgtca tccaccatcg cctgcagaac cggacgcacc tcatccaccg tcacatgctt
     2161 ctgtaatacc gccgacaggg aagtcagttt ctcttcaaac gcttgtgcct gcgaggccat
     2221 cttcccctca aatgtgcgct gtaaatccgc cagcactgtg gaaaattctt ctcccagtgc
     2281 acgaataatg gacagttccc gttccgtcat tttctcagta tcccccctga acatcgcttt
     2341 caccgcatca tgctctgttt cactgattgc cttattaccg tcagatgcgc cgtcaggcag
     2401 ctgtgatgaa actgttttcc cggcagacgc gaacggatcc tcacgggcat cacgacggga
     2461 cagcgcctcc agactgtagt tctgctgctg aagatacagt gcatcaccgc cggccagggg
     2521 cggcaggttc tcccgtttac gggcctcatt gggcgtgaga agcgtatttt tcaccgcatc
     2581 ccccagcgtt ttcatgcgcc gctcactgtc cattctcagc agcgtggtga catcaaattc
     2641 tgtactctcg ttttcccccg tttccagcgc ctcatccagt aacagttcaa tggactcaat
     2701 cagcgtctgc aggcactggg aataatactg ctgctccagc gcctccacgt tgtcactgga
     2761 aggcggttgt cccacgccaa tcttgtaggc cgggacacgg aacaccgaac agacaatttc
     2821 agcggtcatc ttcagttgtt ccaccgtctg cgcatccacc ggtgaaaacg tcgtggggtt
     2881 gtattttgcc ccgttgctca gaatggccgt tttccccgca ttttcgcctg tatacccgct
     2941 gtcccagttg ctcttcagtt ttttcgcatt ttcttccgta atactgccgg ggatctcaat
     3001 caccccggac ggcctgccgc catttctgaa aaaagacgtc gaattttcct gaatatgatg
     3061 cccctgcgtg gccgccagcc cggcggcata caccggcggc aatcctataa gcggatgaaa
     3121 aaaacagtta aaccggtcgt ggatcacttc ccgggcaggc accgtcaccg cctccgtgat
     3181 cccgcagttc cggtccggcg tgatgcggta gaacacctcg ccgtcatccg ccaccagagg
     3241 ttcaacccgg ctccagtcca gaatacgcag ttctttgatc tgcccccggg cattacggat
     3301 tttcagcacc accgtattgc catgacgcag tttggcgttc agccacagtt caaaaaactg
     3361 gatgcggttc tgctgggcgt tgggacgacg acagaggcgg gcaatatccc cccggcgcgt
     3421 ttccctgcgt atcccatgcg catccgtctg cataagacgc agccgcattt tggcgatatc
     3481 ctgggatatc agcgaaatac atgcaaacac cgcatgaaag gagaggacgg cttcaggatc
     3541 ggctttcacg ccctgctgcc aggcgccgga aaagggctca gccaccgcct gaaacaggct
     3601 ggtccagccc gcctctctta cgtcacgtcc tgatttctgg ttttttcggg ttcgccgtaa
     3661 aaggttccac attcgccatg ctccgcatca cgtttctttt tctgacctgc cggacgtcgc
     3721 accgtgatgt actccgcctt tcccaggcga accagcacct ccgcacacgg ctgtgccaca
     3781 tcacggatat ccccggcccg ggcatcatgc gtgccctgca gatatcggat ctttgccata
     3841 acctgttacg ggaggcgcac gcctcccgtc ctccttatca gactcagccg ccggacgcac
     3901 tgccgtagtt cactccggtg atcaccgcca ccgccgcagt acggcgacga cgccagttga
     3961 tccagcgctc cgcacggatg gccacgctgc ctgtctggaa catggaaacc agctccaccg
     4021 gggacggcgt ggtactgtcg ccgcccggct cagactgcat ttccagtgat gcctcgcggg
     4081 acatatccac tgccacgccg ccgtcatccg ccagataaat atccggggca ttcaccagca
     4141 ccagctggtc acccacgtac tgggagacaa tcaccggcag cccctggaag gagccaccca
     4201 gcagggtcat gtccgggtat tccttctgac ccagcgcatt tttacgcatg gacagtgcca
     4261 gggcattggt gctggacatc agccagaccg caccggtggg ctgcaggttt gctgccacaa
     4321 actgtccaaa cgcagcctcg gcatccgcat ccgggttacc ggttgatgcc gtgcccttca
     4381 catcatgggt gatggacgcc ggggagacat ctgccactgc ggcttttttc gggtccacaa
     4441 agtctgtatc cagacgcgcc accaccgctt ccgccagcgc attacggacc agtgcatcag
     4501 cagccggact ggaaaaacgg atcaactctt ccgtcagtac cgcaatggcc gacaccttcg
     4561 catgactgaa ggtgatggat tcaaaatcaa acttcgtcag gggttttgcc ttaccctcac
     4621 ccacccagcc ggcagcaccg ccggacacct gggcgtgcac acggatattg aacggcacct
     4681 gacgaagtgc agggatcccg ccctgaccaa atcgcccgat aatggtctgc ggacgcaggt
     4741 aatcaataaa gtcctgtgcg tattcctgat attcagacag gctgcctgcc cactgcggat
     4801 ccgtggtggt ccccgcgccc actgccgatt tcaggacatg atgcagacga ctgtcatccg
     4861 gatactgacg acgggccact tccagggctt cagatcggac gcctttagcc gcagccagcg
     4921 atttggcaaa gcgggcgaag ccaatcccct tatccagttt ctgctccaca cggatcaccg
     4981 gcgcagaagc caccgcggcc acattcccgt taccggcctg tttcaccggc tgcgccgtgg
     5041 cggccttacc ggcttccagt tcacgcaggc gcttcaggtg cgcatccacc tgacggattt
     5101 ccgctgcggt gttgtcgtaa tgctcttcct cctccacatc cagcgtgcgc ccttcctctg
     5161 cggctttggt catgacctcc tcaagggagg ctgccagcgc tgcacgcttg ttttcaaaac
     5221 ttttaatctg ttcgccaata ttcattatgg tcttttcctt atgaaaaacg gttgttgact
     5281 gtgccgcagc gccggcagaa gatgcgattt tcaccaccgg tttccggttg ccggacgcgg
     5341 cagaaaacgg gcggtcgtaa gatttaatgg tccggatggt gcattccgca ttcgcgggca
     5401 cggtgacggc agacacctcc atcagttccc agcgcagaaa atgcagtccg cctccgtcca
     5461 gaaaggtgta ttcatgggga cggaagccca cggacagccc cctgaccagc ccggtcttaa
     5521 tggccgccca gacctcatcc agccgggcag ccagttgcga cggcatatcc ggtacgggct
     5581 tcaccagtgt tgccgtgatt tccagccctt cgctgacccg gcgcaccgta cactgcccca
     5641 ccgggcggga atggtcatgc tgccagagaa acgggatcgc actgccaaac tccgcgccct
     5701 ccggctccag gatgtcacca tcccgatccg gagaaggcgt tgacgcaatc ccggtgatca
     5761 cccgttcatc ctcactgaag gatttcaccg tcagcaggga acaggcccgt ttaagagtca
     5821 catcagcctc ctgaaaataa aaaaaccgcc gcagcggttc atgatggtta cagtgtgaac
     5881 agggttatat gaaaaaaacc gcatattctt tctttttcgg ttccgggtta agggacatca
     5941 gggagaccgc attgaacagc gccatcagcg ggtcaatttt tccccgtcca ctggcctgtt
     6001 tggtgataag aatggcgtta cctttaggct ccacccgggc attgccgaca caccaggcca
     6061 tcaggggctg gtcaccatgc accagcaccc cttcagccag tttgcgctcg gtggttttaa
     6121 tggccccgcc cagtttccag ccctggctta tccccaccac aattccgtcg gggatcccgg
     6181 cttccgccag tgaatccaga atctgcccca cccctgacgg gtcaataccg atatggtcca
     6241 gtaactcagc ctcatgaatg cgacgcacat attccgccac ttccgccgtg tcatccccga
     6301 cacgccggac aatggtcata tctccacagg caacaagatc ctgaaaccgg gacgcctcgc
     6361 tcttccgtcg gaccaccgcg gtttcatgcg cccaggcatg gccccagccc agccattcgc
     6421 gggtttccct gtcacggcca atcacgtaca ttcccagcag atcatccagg cccccgccgt
     6481 caatccccac cgtcaccaca tcagcgcgct gcaggatatc gtccaggctg acgcgcctgc
     6541 cctgctgctc ccagaaatcc gcacccgccc agcggtcaga acgcagggca agaccaattt
     6601 ccacattggc atgttttgac atgaagccac gaaatgcttc ctcaccagcc tcccgggctt
     6661 tacggtactc ccggtacaga aaagcctcat ccaccgaata accgagattc gggttaacca
     6721 tggcgaggtt ttccatcagc aggtgagccc cgctttccac catttcagga ggatgctcaa
     6781 aaatcaccgg cagaaagtgc ggatcatgaa ttttgccgtc acggacatcc cgggcg
//
LOCUS       DBFIFM010000221         6822 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.221, whole genome shotgun sequence.
ACCESSION   DBFIFM010000221 DBFIFM010000000
VERSION     DBFIFM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.221"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..236
                     /gene="yafQ"
                     /locus_tag="N7C74_004087"
     CDS             <1..236
                     /gene="yafQ"
                     /locus_tag="N7C74_004087"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HGU2433851.1"
                     /translation="VKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGY
                     RDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            392..1132
                     /gene="dpaA"
                     /locus_tag="N7C74_004088"
     CDS             392..1132
                     /gene="dpaA"
                     /locus_tag="N7C74_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HGU2433852.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1103..1870)
                     /locus_tag="N7C74_004089"
     CDS             complement(1103..1870)
                     /locus_tag="N7C74_004089"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HGU2433853.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGKTDSEKAFCWLLYKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(1975..2553)
                     /gene="lpcA"
                     /locus_tag="N7C74_004090"
     CDS             complement(1975..2553)
                     /gene="lpcA"
                     /locus_tag="N7C74_004090"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HGU2433854.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2793..5237
                     /gene="fadE"
                     /locus_tag="N7C74_004091"
     CDS             2793..5237
                     /gene="fadE"
                     /locus_tag="N7C74_004091"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HGU2433855.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTLGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNVFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5280..5726)
                     /gene="ivy"
                     /locus_tag="N7C74_004092"
     CDS             complement(5280..5726)
                     /gene="ivy"
                     /locus_tag="N7C74_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HGU2433856.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            5907..6677
                     /gene="yafV"
                     /locus_tag="N7C74_004093"
     CDS             5907..6677
                     /gene="yafV"
                     /locus_tag="N7C74_004093"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HGU2433857.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWRDADEFRLR"
BASE COUNT         1614 a         1732 c         1816 g         1660 t
ORIGIN      
        1 atgtaaaact tgcacaaaag cgtcataagg atatgaataa attgaaatat cttatgacgc
       61 ttcttatcaa taatacttta ccgcttccag ctgtttataa agaccacccg ctgcaaggtt
      121 catggaaagg ttatcgcgat gctcatgtcg aaccggactg gatcctgatt tacaaactta
      181 ccgataaact tttacgattt gagagaactg gaactcacgc ggcgctcttt gggtaaagcc
      241 cgctgccgat gaaatttgta aaccctgcgc cgacaggctt gcgtaaaact cttttggtga
      301 taatgtgagc cttcccaatc cagactattc cgatttggga tccattttgc cgtcctggcg
      361 tgtgtaaccg ttttatcaag gaataagcag tatgcgtaaa atcgcattaa ttcttgcgat
      421 gttgttgatc ccgtgcgttt cgtttgccgg tttgctgggc agcagtagct cgaccacgcc
      481 agtgagcaaa gagtataagc agcagttgat gggatcccct gtctacatcc agatcttcaa
      541 ggaagaacgt acgctcgatc tctacgtcaa aatgggcgag caatatcaac tgctcgacag
      601 ctataaaatc tgtaaatatt ccggcggctt agggccaaaa cagcgtcagg gcgatttcaa
      661 aagcccggaa gggttttata gcgtccagcg taatcagtta aaaccagaca gccgttacta
      721 caaagcgatt aatattggtt tccccaatgc ctatgaccgt gcgcatggtt atgaagggaa
      781 atacctgatg atccacggcg attgtgtttc catcggctgc tacgcgatga ccaatcaggg
      841 cattgacgag attttccagt tcgttactgg cgcactggta tttggtcagc cgagcgtgca
      901 agtgagcatt tacccgttcc gcatgaccga cgccaatatg aagcgccata aatattccaa
      961 ctttaaggac ttctgggagc aactgaagcc aggctacgac tactttgagc aaacccgtaa
     1021 gccaccgacc gtttctgtgg tcaatggccg ttacgtggtc agcaagccgt taagccacga
     1081 agttgtgcaa ccacaactgg catcaaacta cacgctcccc gaggcaaaat aagcgccatt
     1141 cgcctggcat aatcttttgc caggtttcat tgcccgtcag cggctgtgtc gcaatcaccg
     1201 tgaccacatc attcggtgtg gtctgcgagc tgaagtcgat ttccacatcc tgatccagca
     1261 acgttgccac gccaaacggc gcgcggcggg tgatccagtg cagattggtc gagcaatacg
     1321 ccattacata gcgcccatcc gaaagcagca tgttgaaaac gcccttctgc cgcagttcat
     1381 ccgccagtga ggcgatatat ttaaataccg ccgccatatt gcccggcgta cgcggataac
     1441 gctgcgttaa tttatacagc agccagcaaa aggctttttc gctgtcggtt ttacctaccg
     1501 ggcggaagtt gccggtttcc agtgatttat agcccgtcag ttgtccgtta tgggcataag
     1561 tccagttacg tccccataac tcgcgggtaa acgggtgagt attttccaac gccacctcgc
     1621 cccgatttgc ctggcgaata tgagccacaa ccgaacagga ttttatcggg tagtcctgaa
     1681 caagtttggc aatgggggaa ttaaagctgg gttgtggatc tttaaatgtg cgacagcctt
     1741 taccttcgta aaaggtaatg ccccagccat ctttatgtgg cccggttcca ccaccacgct
     1801 gcaccagccc ggtgaaacta aagcaaatat cggtagggac gttggcgctc atcccgagca
     1861 attcgcacat cggtaacccc cacaacagcg ggagcgcatg gcccccggcg aagattgact
     1921 taagaatttt caggccggat gcggcgtaaa cgcattattc ggcctacgcc agacttactt
     1981 aaccatctct ttttcaatta actggatcag gatatggatc actttaatgt gaatttcctg
     2041 aatgcggtcg gcataaccaa aatgtggaac gcgaatttca atatccgccg taccagccat
     2101 tttgccgcca tctttgccgg tcagggtaat cactttcatt cccttctcac gcgccgccgc
     2161 gatcgctttg atcacgtttg cagagttacc agacgtggag atccccagca gtacatcgcc
     2221 ttcgcggcct accgcttcaa catagcggga gaaaatatca ttgaaaccaa aatcgttacc
     2281 gacgcaggaa atatggctga cgtcagagat ggcaatcgcc gggtagcccg gacggttttc
     2341 acggtagcga ccggtcagct cttcggcaaa gtgcatggcg tcgcaatggg agccgccgtt
     2401 gccgcaggaa agcaccttgc caccggcttt aaagctgtct gctaacagga ccgccgctcg
     2461 ctgaatggcg tgaatattgg cgtcatcttt taaaaagtta gccagcgttt ccgccgcttc
     2521 gttcagttcg ttacgaataa gatcctggta catgaggata tccttcagca taaatgtaat
     2581 agacaaaatg cagtgtaccg gataccgcca aaagcgagaa gtacgggcag gtgctatgac
     2641 caggactttt tgccctgaag tgtagataaa aacagcaaca atgtgagctt cgttgtaatt
     2701 atattgtaaa cacattgcta aatgttttta catccactac aaccatatca tcacaagtgg
     2761 tcagacctcc tacaagtaag gggcttttcg ttatgatgat tttgagtatt ctcgctacag
     2821 ttgtcctgct cggcgcgttg ttctatcacc gcgtgagctt atttatcagc agtctgattt
     2881 tgctcgcctg gacagctgcc ctcggcgtcg ctggtctgtg gtcggcgtgg gtactggtgc
     2941 ctctggccat tatcctcgtg ccgtttaact ttgcacctat gcgtaagtcg atgatttccg
     3001 cgccggtatt tcgcggtttc cgtaaggtga tgccgccgat gtcgcgcact gagaaagaag
     3061 cgattgatgc gggtaccacc tggtgggaag gcgacttgtt ccagggcaag ccggactgga
     3121 aaaagctgca taactatccg cagccgcgcc tgaccgcaga agagcaagcg tttctcgacg
     3181 gcccggtaga agaagcctgc cggatggcga atgatttcca gatcacccat gaactggcgg
     3241 atctgccgcc ggagttgtgg gcgtatctga aagagcatcg cttcttcgcg atgatcatca
     3301 aaaaagagta cggcggcctg gagttttcag cttatgccca gtctcgtgtg ctgcaaaaac
     3361 tctccggcgt cagcgggatc ctggcgatta ccgtcggcgt gccgaactca ttaggccccg
     3421 gcgagctgct gcaacattac ggcactgacg agcagaaaga tcactatctg ccgcgtctgg
     3481 cgcgtggtca ggagatccct tgctttgcac tgaccagccc ggaagcgggt tccgatgcgg
     3541 gcgcgattcc ggacaccggg attgtctgca tgggcgaatg gcagggccag caggtgctgg
     3601 ggatgcgtct gacctggaac aaacgctaca ttacgctggc accgattgcg actgtgcttg
     3661 ggctggcgtt taaactctcc gacccggaga aattactcgg cggtgcagaa gatttaggta
     3721 ttacctgtgc gctgatcccg accaccacgc cgggcgtgga aattggtcgt cgccacttcc
     3781 cgctgaatgt gccgttccag aacggaccga cgctcggtaa agatgtcttc gtaccgatcg
     3841 actacattat cggcgggccg aaaatggccg ggcaaggctg gcggatgctg gtggagtgcc
     3901 tctcggtagg ccgcggcatc accctgcctt ccaactcaac cggcggcgtg aaatcggtag
     3961 cgctggcaac gggcgcgtat gctcacattc gccgtcagtt caaaatctct atcggtaaga
     4021 tggaagggat tgaagagccg ctggcgcgta ttgccgggaa cgcttatgta atggacgctg
     4081 cggcatcgct gattacctac ggcattatgc tcggcgaaaa acctgccgtg ctgtcggcta
     4141 tcgttaagta tcactgtacc caccgcgggc agcagtcgat tattgatgcg atggatatta
     4201 ccggcggtaa aggcattatg ctcgggcaaa gcaacttcct ggcgcgtgct taccagggcg
     4261 caccgattgc catcaccgtt gaaggggcta acattctgac ccgcagcatg atgatcttcg
     4321 gacaaggagc gattcgttgc catccgtacg tgctggaaga gatggaagcg gcgaagaaca
     4381 atgacgtcaa cgtgttcgat aaactgttgt tcaaacatat cggtcacgtc ggtagcaaca
     4441 aagttcgcag cttctggctg ggcctgacgc gcggtttaac cagcagcacg ccaaccggcg
     4501 atgccactaa acgctactat cagcacctga accgcctgag cgccaacctc gccctgcttt
     4561 ctgatgtctc gatggcagtg ctgggcggca gcctgaaacg tcgtgagcgt atctcggcac
     4621 gtctgggtga tattttaagc cagctttacc tcgcctctgc cgttctgaag cgttatgacg
     4681 acgaaggccg taatgaagcc gatctgccgc tggtgcactg gggcgtacag gatgcgctgt
     4741 atcaggcaga acaggcgatg gacgatttac tgcaaaactt cccgaaccgc gtggttgccg
     4801 ggctgctgaa tgtggtgatc ttcccgaccg gacgtcatta tctggcaccg tctgacaagc
     4861 tggatcataa agtggcgaag attttacaag tgccgaacgc cactcgctca cgtattggtc
     4921 gcggtcagta cctgacgcca agcgagcata acccggttgg tttactggaa gaggcgttgg
     4981 tggatgtgat tgccgccgac ccgattcatc agcggatctg taaagagctc ggtaaaaatc
     5041 tgccatttac ccgtctggat gaactggcgc acaacgcgct ggcgaagggg ctgattgata
     5101 aagatgaagc cgctattctg gtgaaagcgg aagaaagccg tctgcgcagt attaacgttg
     5161 atgactttga tccggaagag ctggcgacga agccggtaaa gttgccggag aaagtgcgga
     5221 aagttgaagc cgcgtaattg aataacggag ccgaaaggct ccgtttcttt atccgctaat
     5281 tatttaaaat taaagccatc cggatggttt tccaggctgc cggtcaacgc cgcgaacagc
     5341 accgttttac catcaatcga aagcgcatcg ttcacattca accaggtcag tttctcttgc
     5401 gacgttttct catcaatagt cgagaacagc cccgtcatct gattagattt ctcggaccac
     5461 atcacagcga tacgttgcga gccacagtca tgcggtttgc acgcgctcat cacctgatac
     5521 gtctcatctc ccaacgttac ggtttgtgca ggggtataag taccgccttt catcacccag
     5581 gcaggcagct tatgcccttg caccatctga ttaaatgcag ctttggtggt ttcgcccttt
     5641 gcaaggctgc taatggttaa atcatcctgc gccattgcac tggtggcgat gaccagtgcg
     5701 gcgactgtcg ttattgcctt aaacatcatt cctcccgagc ttatcctgcc catgttatta
     5761 acctcctaaa gcctggcgat aacacgataa tttgcaaatc agatatttcc aatatcagca
     5821 tttgttatca atttattctg cgctaaggca actgcctgat aaggtgttaa agttataaaa
     5881 cccttcgcat aaaggagttg tacaccgtgc ctggtttgaa gattacgctt ttgcagcaac
     5941 cactggtgtg gatggatggt cctgccaacc tgcgtcattt tgatcgtcaa ctggaaggta
     6001 ttaccgggcg cgatgtgatc gttctaccgg agatgtttac cagcggcttt gccatggaag
     6061 cggctgcttc gtcgctagca caagatgacg tagtgaactg gatgacagcc aaagcgcagc
     6121 agtgcaatgc gctgattgca ggcagtgttg cattacaaac ggagtctggt tcggttaacc
     6181 gctttttgct ggttgagccg ggcggcacgg tacattttta tgataagcgt catctgttcc
     6241 gcatggcaga tgagcatctg cattataaag cgggcaatgc gcgagtgatt gtggaatggc
     6301 gcggctggcg tattttgccg ttggtgtgct acgacttacg ttttccggtg tggtcgcgca
     6361 atctcaacga ttatgacctc gccatctacg ttgccaactg gcctgctccg cgctctctgc
     6421 actggcaggc attgctgacg gcccgcgcga ttgagaatca ggcgtatgtg gcgggatgca
     6481 atcgcgtcgg cagcgatggc aacggctgcc attatcgcgg tgacagccgg gtgattaatc
     6541 cacaaggcga gattatcgct actgccgacg cgcatcaggc aacgcgcatt gatgcggagc
     6601 tgtcgatggt ggcattgcgg gaatatagag aaaagtttcc ggcatggcgg gatgcggatg
     6661 agtttaggtt gcggtgagtg ggggctgtat ttagggcggg gaattcagaa agggatgttt
     6721 agtgtgctga gcgagagtta aataataaaa aactgggaag atttaataat ccccccgcag
     6781 ggcaagagca tgaattaaat ctgtgttaaa tttttcattt tg
//
LOCUS       DBFIFM010000222         6757 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.222, whole genome shotgun sequence.
ACCESSION   DBFIFM010000222 DBFIFM010000000
VERSION     DBFIFM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.222"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..219)
                     /locus_tag="N7C74_004094"
     CDS             complement(<1..219)
                     /locus_tag="N7C74_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433858.1"
                     /translation="MSMVEWISHEKKDSLILFVHGLNGGLETWNFNKETSFPKLLAED
                     EDIGNVFDIACFNYFTKFTQTYAKTSGLW"
     gene            complement(309..2591)
                     /locus_tag="N7C74_004095"
     CDS             complement(309..2591)
                     /locus_tag="N7C74_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HGU2433859.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDHIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYGELSDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKIYRKGQMTTRQAYSAIARLFNDE
                     WWTHQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVRKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGKPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDD
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPTEHAAAVLNLIDEGILSWTEPGIMKVLRDLLSNELKCSNRS
                     QQSFTPFNGRSYFPAPSARLTRQERKTIPKIKVLLAQSDIQASYWELEALARGAVLDF
                     GHKRFKFDNDIDFFDRQREW"
     gene            complement(2581..2856)
                     /locus_tag="N7C74_004096"
     CDS             complement(2581..2856)
                     /locus_tag="N7C74_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HGU2433860.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGR"
     gene            complement(2853..3077)
                     /locus_tag="N7C74_004097"
     CDS             complement(2853..3077)
                     /locus_tag="N7C74_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HGU2433861.1"
                     /translation="MPDNVDFIQEQQAELLERQINAARVKHCGASALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKRYRRTA"
     gene            complement(3080..3379)
                     /locus_tag="N7C74_004098"
     CDS             complement(3080..3379)
                     /locus_tag="N7C74_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HGU2433862.1"
                     /translation="MSCSHSVVLLNNALKIAVMKNGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTELASYCADVSRKLNDKRS"
     gene            complement(3379..3603)
                     /locus_tag="N7C74_004099"
     CDS             complement(3379..3603)
                     /locus_tag="N7C74_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HGU2433863.1"
                     /translation="MHTVSENKCGKYALLLQQARAEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(3667..4167)
                     /locus_tag="N7C74_004100"
     CDS             complement(3667..4167)
                     /locus_tag="N7C74_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HGU2433864.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(4164..4334)
                     /locus_tag="N7C74_004101"
     CDS             complement(4164..4334)
                     /locus_tag="N7C74_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433865.1"
                     /translation="MNEPRCIAQLLRNESPRAIDFTITHGKGRKGIIIRTKKQSPLKK
                     ALTFLKSRRVWK"
     gene            complement(4345..4620)
                     /locus_tag="N7C74_004102"
     CDS             complement(4345..4620)
                     /locus_tag="N7C74_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory phage cox family protein"
                     /protein_id="HGU2433866.1"
                     /translation="MNRQLVSVTDAVPYQEFAKLIGKTPRAVRGMIEKGKLPVIEITD
                     PQSVSGRAGEYWVYLPAWNNGLKLAYESRPKEIRDGWLMWLGLGEPR"
     gene            4742..5041
                     /locus_tag="N7C74_004103"
     CDS             4742..5041
                     /locus_tag="N7C74_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2433867.1"
                     /translation="MSIDVSEKLKLIRESERLNRKEFSELTGVAYSSLSSYESRSKNA
                     GVEAIMKVLQHPRFTKYTLWFMTDQVAPEAGQIAPALAHFGQNETTSPHSGQKTG"
     gene            5157..6170
                     /locus_tag="N7C74_004104"
     CDS             5157..6170
                     /locus_tag="N7C74_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HGU2433868.1"
                     /translation="MAIKKLDDGRYEVDIRPTGRNGKRIRRKFDKKSEAVAFEKYTLY
                     NHHNKEWLSKPTDKRRLSELTQIWWDLKGKHEEHGKSNLGKIEIFTKITNDPCAFQIT
                     KSLISQYCATRRSQGIKPSSINRDLTCISGMFTALIEAELFFGEHPIRGTKRLKEEKP
                     ETGYLTQEEIALLLAALDGDNKKIAILCLSTGARWGEAARLKAENIIHNRVTFVKTKT
                     NKPRTVPISEAVAKMIADNKRGFLFPDADYPRFRRTMKAIKPDLPMGQATHALRHSFA
                     THFMINGGSIITLQRILGHTRIEQTMVYAHFAPEYLQDAISLNPLRGGTEAESVHTVS
                     TVE"
     gene            complement(6435..6752)
                     /gene="yegR"
                     /locus_tag="N7C74_004105"
     CDS             complement(6435..6752)
                     /gene="yegR"
                     /locus_tag="N7C74_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HGU2433869.1"
                     /translation="MKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         1841 a         1690 c         1455 g         1771 t
ORIGIN      
        1 ccataatcct gacgttttag catacgtttg tgtaaatttg gtgaaataat tgaaacatgc
       61 aatatcaaat acattgccaa tgtcttcatc ttctgctaat agttttggaa atgaagtttc
      121 tttattaaag ttccatgttt ccaaaccacc atttaatcca tgtacaaata gtatcaggct
      181 atcttttttc tcgtggctta tccattcaac catgctcata tgcttttctg ccgtgtataa
      241 aaattaaact ataacaaaca attcctcaac aataacatat tgttaccccg tttgaccgtt
      301 tttttcaact accactcacg ctgtctgtca aaaaagtcga tatcgttatc aaatttaaaa
      361 cgcttatgcc caaaatccaa aacagctccg cgagcgagag cttcaagctc ccaataacta
      421 gcctgaatgt cactttgtgc aagaagaacc ttaattttcg ggatagtctt tctctcctgc
      481 cttgtcaacc gggcggatgg ggcaggaaag tagcttcgac cattgaaagg ggtaaagctc
      541 tgctgtgaac gattactgca tttcagttca ttactcaata agtctctaag taccttcata
      601 atgcctggct cggtccaact tagaatccct tcatctatta aatttaacac cgctgcagca
      661 tgctcagtcg gtgtgggagc cggtaacgaa gtatcaccac cggtgagttt tccacagtta
      721 ttgacaggac tccgaggcgc ggcgatgccg ctttttaaag tcaaaggctc aacgaccgga
      781 actttcggca caatgcgcca gtccgtcgtt ctggtgatat gaatatgacg cgcgccgaga
      841 tgcggcgcgt aaatgccgac cactctctcg acttcttcct cgtactcgtt aacgtcatcc
      901 gacgggctac gggcgaccct gacagtctga caatcgcgcg ggacatttgc cccaccctgc
      961 gcgctgatat acaacgcaaa atcaccactg tctgcggcag cgcgagcagc ctcgacgcgt
     1021 tcgtcaaact catcagcaat actgacgccg cgaggcaatt tgcgtagttc acggtaagcc
     1081 cccattgtcg gcagtccaac cgttttaaat tgcggaatgc gccacgttga cgcccatgcg
     1141 gtaacagccg cggcagtatc tttaagcggc ttaccggtat cgttatcgag ctgaccatcc
     1201 agtgcatagc cgtcgatgtt ttttgaaatg tatttcgcga tatatcccgc agcaccgccc
     1261 cggttaaggt gttttgcctg aaaacggttt cgcgcagctc ctctttcgtc tccatcctct
     1321 ttgagcgcgt agcgacgcat gatttcgata atctggttac gctggcgtgg attacaaaaa
     1381 agcatcatat gccagtgcgg cgttccgtcg tggtgtggct cgacgacacg caaaccgtag
     1441 acctgtaaat cattatcctt gaatgccgtg cgcatcaggc tccagatgcg gcagagataa
     1501 cgctgcgcat cctttggatt aaatgcctca tcgttccagc cgtgattaag ctgaacggtt
     1561 ttactttcgc cttttctgac ctgacgtgtc gggtgatact ttgacggcgc ggtcagcgtg
     1621 ataaacatcc ccacatcacc ctctgcggcg gcgtaacgct caataccggc aatggtgttc
     1681 atcagctcca tccggcgaat ttcaggatta gaaatactgc ccatcacctt actgataagg
     1741 tcgatgcgct cgccggtttc cctgttttca aggtcacacg atttaagaaa ttccagattt
     1801 gcctggcggc gcgcacgcac atcacgaatg gcgtgtttac tggcataagg agaacggtct
     1861 ttattgacct ccccgacagc tatcagtaac gcttcatgcc agcgcatacg ctggccttta
     1921 agctgatgag tccaccactc atcgttaaac agacgggcaa tggcagaata tgcctgcctc
     1981 gtggtcatct gccctttacg gtatattttc cagtagagag gggaaatatt gaaagcacgt
     2041 gcagcgccag caacatgacc atagaggtga gcctgcgcct catccgtaaa cagcgattct
     2101 ttttcgccat gcgcatccac ccaggcatca ctgagttccc catacatcat gaaaagctgc
     2161 gatgagatac gggcagcaaa ctttttcagc tccttgtcat tcattcccgg caggcgcgca
     2221 tagtggtcac gctctgccag aaacagtaac gacgcgtcgg tgttcatttc atggcgctga
     2281 ttcacacgct caatgcgcgg ccataaacga cgctgaaaag tggatgtgag gaaataaaac
     2341 ccgtgcaccg ggcttttatt gcgccggatg tggtcatagc gtgaagtaaa cagcgagcgc
     2401 aaaaagtaag gcaggcggtt aatcgtggat aaaacacctt gcacctgacg catctcgtca
     2461 cgtgtaaggg gtctttcgcg cccgacggcc tcgcgtggcg cgttccatgc ataagcaccg
     2521 gtaaacgcct taccggtgcc tgcagcaaat gctgacggag ggacaaaacg cccggaggct
     2581 ttaacggcca tatgagccaa aagcctctga gcaacgcttg ctgagttgct caacctgcgc
     2641 gtttaaatca gcaaaagact ttgcgcttcc ggtcagaata tcgtgatgca tcaggccgga
     2701 aacgagctgg cttaatttcg ggtaataacc aaccaccgcc agccattcct gaccggcgtt
     2761 tttaccgctt tccgctctct ttttctcgtg gagaataaac tgaaagctgt cactggtaac
     2821 gacataacgt tcgccaattt caatacgaat actcatgccg ttctccggta acgtttgttt
     2881 tttgcttcaa agactgactg acaggaaaca caacgcgtgg ctgacgggta agccgcacga
     2941 cgggcagcag gtattggcgc gtcacactct tcgcaaacca gcgcagaagc accgcaatgt
     3001 tttacccttg ccgcgttaat ctggcgctcc agtaattcag cctgttgttc ctgaataaaa
     3061 tctacgttgt ccggcattat cagctccttt tatcgttaag tttcctggat acatcagcgc
     3121 aataactggc aagttctgtc gttaattttg tcagttcatc cactgaggaa atttgcttgt
     3181 ggaatacagc gcgtttaaca agtaaattga ccacatcaga caggaggttt aattcattct
     3241 gataaatcgc gataacagat tcagttatgt cgcgtttttc tttatcaagg caaagttgaa
     3301 taagagacaa atcgccattt ttcataacgg cgatttttaa ggcgttattc agtaatacaa
     3361 ctgaatgaga acaggacatc aaagcacctc cccgcgagac aatccgatat tgtgaaattt
     3421 ttccgactcc tgactgagca gctcgactat ctccacgcgg gataactccg cctttgtgat
     3481 atggcgaatc atggcgtcaa gatgagaaga aaagcgtgtc gctgcgtcgg cctgtgcttc
     3541 ggctctggcc tgttgcagca gtaatgcgta tttaccgcac ttgttttcag aaactgtatg
     3601 catgactttc tccaggcaaa aagaagcccc gcacgattaa gtgcgttaaa aactctggtt
     3661 aattatttaa tgcagatatt gctctggttt taccgacgtc agaattgtcg gtgcatactc
     3721 aaacaggctg aataattcac gtaatgcacg gaataaagca tcacgccagt aacatgattc
     3781 ttcattaatt cgccagtatg gctggttgaa ttctttttca gtcaatccgg catgcataaa
     3841 taaagtacga cgctgactga ctgttaaaaa actaatatat gcatactcac ttgcgccaac
     3901 ctgacggcgt tttgagaatg ccccacgcaa ttcatcaatt gcacaaacca gccgttcacg
     3961 ttcgacgtcg ttcatttctt caaaacgcat cgttgcgtga cgctgtttta actgcgcatg
     4021 gaagcaaacc gttagccgtt cgcgttccat catctgatta taataatcac atgtatcctg
     4081 ccagcgaggg acggcaagat gcttgccaat tatccggcgc atagctgctg gctgtttttc
     4141 aacgagattg agcgtcatca ctgtcatttc cagaccctcc ggcttttcag aaaggtcaga
     4201 gcctttttta acggactctg ttttttggtg cggataatga ttcccttacg ccccttaccg
     4261 tgggtgatgg tgaagtcaat cgccctgggg ctttcgttac gcaataactg agcaatacaa
     4321 cgaggctcat tcatacggtt ctccttaacg tggttcaccg agacctaacc acatcaacca
     4381 gccgtcacga atctctttag gacggctttc ataagccagt tttagtccgt tattccatgc
     4441 cggaaggtat acccaatatt caccagcacg ccccgatact gactgagggt cagtaatctc
     4501 aataactggt aatttccctt tctcaatcat gccccttaca gctcttggag ttttaccaat
     4561 gagttttgca aactcctgat aaggcacggc atcagtcacg cttacaagct gtctattcat
     4621 ctgctacgat tctcccttag tgcttctaat ggctcctaat ggctaattat tgcctaaaag
     4681 gataactcca gaagcacaac atttcacact atcagcaaga aattacgcaa tcggagtaat
     4741 tatgtcaata gacgtttcgg agaagttgaa gctaatccgt gaatctgaaa ggttaaaccg
     4801 taaagaattc agtgaattaa ctggtgtagc ctacagctca ctttcgagct atgagagccg
     4861 gtcaaaaaac gctggagttg aagccataat gaaggtctta caacatccta gatttactaa
     4921 atatactttg tggttcatga ctgatcaggt agctccagaa gccgggcaaa ttgcgcccgc
     4981 tctcgcacac tttgggcaaa acgaaacaac gtcgccccac tccggtcaaa agactggtta
     5041 acaatttatc gtgaatatat tcatcacaag tgcctactat tggtggctaa atttcagcca
     5101 ccacgaaaaa agcgattagt agtagcaaaa aaaagtacca ctcggagggt tttctgatgg
     5161 caatcaaaaa actcgatgat ggtcgatatg aagtggacat ccgccctact ggacgtaacg
     5221 gaaaacgcat ccgtaggaag tttgataaga aaagcgaagc tgtcgctttc gaaaaataca
     5281 cgttgtacaa ccaccacaat aaagaatggc tatcaaaacc aacagacaaa cgacgtctgt
     5341 cggaactgac acagatctgg tgggatttaa agggtaaaca cgaagagcat gggaaatcta
     5401 atcttggaaa aattgaaatc ttcacaaaaa taacgaatga cccatgcgca tttcaaatca
     5461 cgaaatccct tatcagccag tactgcgcca cccgaagaag tcagggtatt aaaccttcga
     5521 gtatcaatcg tgatttaaca tgtattagcg gcatgtttac agccctgatt gaagcggagt
     5581 tattctttgg tgagcatcct atcagaggga caaagaggct taaggaggaa aaaccagaaa
     5641 caggctatct cacacaggaa gaaatagcct tactgcttgc agcacttgac ggcgacaata
     5701 aaaagattgc gattctttgc ctaagtacag gagcacgttg gggagaagca gctcgtttga
     5761 aagcagaaaa tatcatccat aaccgcgtca cgtttgttaa aacgaaaaca aacaaaccac
     5821 gcaccgtccc gatctcagag gctgttgcca aaatgatcgc ggataacaaa cgaggttttt
     5881 tattccctga tgctgattac cctcgcttca gacgaacaat gaaagcaata aaaccggatt
     5941 tgccaatggg gcaagccaca catgcactaa ggcacagctt tgccactcat ttcatgatta
     6001 atggaggaag tattatcacg ctacaacgga tactaggtca cacgcggatt gagcaaacta
     6061 tggtttacgc tcattttgcg ccagagtacc ttcaggacgc catttctctt aatccgctaa
     6121 gaggtggtac tgaggccgag agtgtccaca cagtgtccac agtagagtaa cgtttaaggg
     6181 ctttcagtgg taatttatgc cgctcaaacc cgcattgtac cgttgaaagc ccctactggt
     6241 gacaccctaa atctccctta cacgggctta ttttttatgc ataagcccta tccctgataa
     6301 ccgtcttcca ttgaccacat cgatagaatc tcccttcata gcacgatgcc tttcacgtaa
     6361 cggcatcgtg ctcgcacagg ttccggctaa gcacaaccag aacgcgcatg tttgacgctt
     6421 accaaaaaat attctcactc tccacatttg aatgtcagac gagcgacgcc atgtaatcct
     6481 gcaccttctg tcttcaggtc aactatctgc atttttttgc cctgagtaac acagaaatgg
     6541 gctgcatcat tttttactat attttctgca ccagatattc tgcccctggc taaagaagct
     6601 tcggcttcgg tgtagtattg gttatcgagt ttacgctgaa tattactttt atatgcaaga
     6661 ccaaatttac cgatacttgt ctcatcatta tgcacagcac aaccagacat aagaaaaaca
     6721 ctaattaatg atatagcagc tatctttttc atctcac
//
LOCUS       DBFIFM010000223         6699 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.223, whole genome shotgun sequence.
ACCESSION   DBFIFM010000223 DBFIFM010000000
VERSION     DBFIFM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.223"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..152)
                     /locus_tag="N7C74_004106"
     CDS             complement(<1..152)
                     /locus_tag="N7C74_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HGU2433870.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLA"
     gene            complement(165..1946)
                     /gene="gcl"
                     /locus_tag="N7C74_004107"
     CDS             complement(165..1946)
                     /gene="gcl"
                     /locus_tag="N7C74_004107"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HGU2433871.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(2036..2851)
                     /gene="allR"
                     /locus_tag="N7C74_004108"
     CDS             complement(2036..2851)
                     /gene="allR"
                     /locus_tag="N7C74_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HGU2433872.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(2929..3411)
                     /gene="allA"
                     /locus_tag="N7C74_004109"
     CDS             complement(2929..3411)
                     /gene="allA"
                     /locus_tag="N7C74_004109"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HGU2433873.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            3641..4567
                     /gene="allS"
                     /locus_tag="N7C74_004110"
     CDS             3641..4567
                     /gene="allS"
                     /locus_tag="N7C74_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HGU2433874.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            4636..5730
                     /gene="mnmH"
                     /locus_tag="N7C74_004111"
     CDS             4636..5730
                     /gene="mnmH"
                     /locus_tag="N7C74_004111"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HGU2433875.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            5846..6211
                     /gene="ylbG"
                     /locus_tag="N7C74_004112"
                     /pseudo
     CDS             5846..6211
                     /gene="ylbG"
                     /locus_tag="N7C74_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1696 a         1748 c         1650 g         1605 t
ORIGIN      
        1 ctcgccagca cctgttttaa ttcttcaatg tcgtagtcat aaggaaacat aaattcaacg
       61 ccgcgaaaac cacactgcgc agctttctca aaacgggcga gaaaatcata ttcaccaaat
      121 aacatcgata aattagcaga gaaacgtaac attatttatc tcccttattc atagtgcatg
      181 aagcaggttt cagtcggtgc atccgctgcg ttatcggcga tatcttcaaa ttccatgacg
      241 ttatccagtt cgctgcccat cgaaatattt gtcacacgct cgagaataac ttccacgact
      301 accggtaccc gatattgcgc cattaaggct ttcgcctgtt caaaggccgg tgcaatatct
      361 tccggtttga agacgcgaat agctttacaa cctaaacctt ccgctacttt tacgtggtcg
      421 acgccgtaac cgttcacttc gctggagttg atattctcga aagcgagttg cacgcagtag
      481 tccatgtcaa aggcgcgttg tgactgacga atcaggccga gataggcgtt attcaccagc
      541 acatggatgt acggaatgtt gaactgcgcg ccaacggcta actcttcaat caggaactgg
      601 aagtcgaagt caccggaaat cgccaccaca ttgcgtttcg gatcagcggc acaaaccccc
      661 agcgccgccg gaatcgtcca gcctaacgga ccagcctgac cacagttgat ccagtggcgg
      721 tctttaaaga catgcagcat ttgtgccgca gcgatttgtg acaggccaat ggtggtgaca
      781 taacaaacat cgcgaccaaa ggctttgttc atctcttcat acacgcgctg cggtttcacc
      841 ggcacgttgt cgaaatgggt tttgcgcagc aaagtgcgtt tgcgctgctg gcaatcggcg
      901 acccattctt tacgacacgg cagacgaccc gctttttgca tctcctgcgc cacttcaacc
      961 agcagtgtca gcgccgcttt agcatcagag acaatgccga gatccggaca cagcacgcgg
     1021 ccaatttgcg tcggctcaat atcgatatga acgattttgc gcccttcggt gtatttctct
     1081 accgaaccgg tatgacggtt agcaaaacgg ttaccgatac caaacaccat gtcggacgcc
     1141 agcaacgttg cgttaccgta acgatgcgcg gtttgcagac cgaccatccc ggccatcagt
     1201 tcatgatcgt ccgggataca gccccagccc atcagcgttg ggatcaccgg aacgctggtc
     1261 agttcagcaa actgttgtaa cagtgcagct gcgtcagcat tgattacccc gcccccggca
     1321 acaatcaccg gacgttcggc ctggattaac atttctacag ctttttcgat ctgcatacgg
     1381 ctggcagcag gtttgtagac cggcagcggt tcgtacatgt caggatcaaa ctcgatttcc
     1441 gcaacctgaa cgtcgaacgg taaatccacc agtaccggac ccggacgacc agaacgcatc
     1501 aggtgaaatg cctgttgcag cacgcgaggc accagcgccg cttcacgaac tgtaaccgcc
     1561 attttgctga ccggtttagc aattgcttca atatctacgg cctgaaaatc ttctttatgc
     1621 agacgggcgc gcggtgcctg gccggtaatg cacagaatag gaatggaatc agcagaagcg
     1681 gaatagagcg cggtgatcat gtccgtgccc gcaggaccgg aagtccccag acatacgccg
     1741 atattccctg ccgttgcgcg ggtataacct tccgccatgt gcgaagcacc ttccacatga
     1801 cgcgccagaa tgtgacgaat accgccgtgc ttacgcatcg ctgagtagaa cggattgatt
     1861 gcagctcccg gaacgccgaa ggcggtggtg ataccttctt tctccagcac atacattgcc
     1921 gcgtcaacgg ctctcatttt tgccatttta ttcctacctc tattaattgg aaaaattttc
     1981 caactttcaa attttgtcaa aaaaacccgc tccgcagagc gggatgcgac agacactatg
     2041 gatgtgcttt cagtcccaac gccgtgctga tatcgcgggc ggtgtctctg accagctcac
     2101 cctgactgac aaaacgatct tctgtcagtc ttgatgaagg cccggagatg gagatagcgg
     2161 caacaacgct acctacatca tcgtaaattg ctgaagctat gcaattcaga cctacaacat
     2221 gctcttcttt atctacggta tagcccagtt cacgcgcttg ttccaggtcc ttcagcaggg
     2281 tgggcatatc cacaagcgta gttggcgtaa actgctgcaa accggtttgc agaatgatgc
     2341 tcatcaactc ctcttccgcc agcggataaa gcagcgcttt gcccgcaccg gaagcatgca
     2401 gtggcagacg actgcccagt ggcgcgcaca tcctgaccat cgatttacac tctaactgac
     2461 caattaatac cgcttcattg ccgttacgga tcgcgacatt gaccgtttcg ccggaaagta
     2521 acatcaggcg gcgcataaac ggcccggcga cggagaggac atcgcggtta tggatgtacg
     2581 ccgcaccgac gttaaagaca cctaatccta tatgccacca gcctaactga ctgtcctgat
     2641 agacaaaatc cgctgcctgt aaaaccttca gcaagcgaaa ggtcgtggag agcggcaaat
     2701 ccagattgag agaaatatcg ctaaccgacg aacttccccc acttttttcc agatattgca
     2761 gaatcgcaat tccccgctct aatgcctgtg cgcccttctg tgccacaggc tccgcctgtc
     2821 ctggcctgcc gcgccgtcta acttccgtca tacctacgtt aactccgacc tacaagtgag
     2881 cataaacaat gaacaattag tttatcttat gcaagtcggt tgcaggcgtc acgcaaaaca
     2941 gagttcctgt tcaggaatac tttcaacatc acagttgtca ctgccgccgc gatcgatggt
     3001 cagaaaatcg gtgacgcgct gccaggcgaa aagtgggtga tgccagacgt tacgatggta
     3061 attcactccc tgttcgccgt tggtaataaa cgcccgcagg gttgacaggt ctggtttgtc
     3121 gtcacctaac gctacgacca ccacaaacac ctcacctttc atcgggataa aggcctgagt
     3181 acccagcgga tgacgttcga gttcgtgaat ggtcagcggc agattcgccg gttgcgctcg
     3241 gttaatgctg ataagcgtac ggtcttgctc aagaatctca accagcgcca aatcgtggta
     3301 acgctccacc aggccattgt taatatggaa aaaatcccgt tgctgcgttt cgattacgtc
     3361 gccataagca ctaaaggctt cctgacttaa cggtaatacc tgaagtttca tctttttgtc
     3421 cttgtaacat aattccgttc aaccagcgcc gaccggtcag cttggtttca ataacaggca
     3481 atctaaaacc ttaagagaac aacatcaaca caaaacttgg aatatttttc caaaactaga
     3541 tttagtccac aaattgaaca gggttatcat gtaaaaatac tgataaatca gaggataaga
     3601 aaatcacgca acttgcggat ttaaaagggg atacctgaag atgttcgatc cagaaacctt
     3661 gcggactttc attgcggttg ctgaaacagg aagtttttca aaagcggcag aacgattatg
     3721 taaaaccacg gcgacgatca gttatcgcat taaacttctg gaagagaata ccggagtggc
     3781 gctgtttttt cgcacgactc gtagcgtaac gttgacagcg gctggcgagc atctactttc
     3841 ccaggccaga gactggctga gctggctgga aagtatgccc agcgagctgc aacaggtgaa
     3901 tgatggcgtg gaacgccagg tgaatattgt catcaacaac ctgctctaca acccccaggc
     3961 cgtcgcccag ttgctggcgt ggctgaatga acgttacccc tttacccagt ttcacatctc
     4021 ccgacaaatc tatatgggcg tctgggactc gctattgtac gaaggttttt cactggctat
     4081 cggcgtcacg ggaaccgaag cgctggcaaa tacctttagt cttgatccct taggatcggt
     4141 gcaatggcga tttgtcatgg cggcggatca tccgctggcg aacgttgaag agccgctaac
     4201 agaagcgcag ttgcggcgct ttccggcggt caatattgaa gacagcgccc gcaccttaac
     4261 caaacgcgtc gcctggcgat tgccagggca aaaagagatt attgttcctg atatggaaac
     4321 gaaaatcgcc gcccatctgg cgggcgttgg cattggtttt ttgccaaaat cgctttgcca
     4381 gtcaatgatc gataatcaac aactggtcag ccgggtaatc ccaacgatgc gccctccttc
     4441 gccattgagt ctggcatggc gcaaatttgg cagcggcaaa gcggtagaag atattgtgac
     4501 cttgtttacc cagcgcaggc cggaaatcag cggattttta gaaattttcg gcaacccacg
     4561 cagttaaaaa tcaagtttcc ttgcgcgttg gcgcgtaaaa tagcgcgcaa gctgatttga
     4621 ggttcacttt taactatgca agagagacac acggaacagg actatcgtgc cctgctgatt
     4681 gctgatacgc ccattattga tgttcgcgcc cctatcgagt ttgagcaagg cgcaatgccc
     4741 gccgctatca atctgccgtt aatgaataac gatgaacgcg ccgccgttgg cacctgctat
     4801 aaacagcaag gctcagacgc agcgctggcg ctgggacata aactggttgc gggtgaaatt
     4861 cgtcagcagc gcatggacgc ctggcgggca gcctgcctgc aaaatccgca aggtattctc
     4921 tgctgcgccc gtggcggtca gcgctcacat attgtgcaaa gctggttgca tgcagcgggg
     4981 attgattatc cgctggtgga aggcggttat aaggcactgc gccagaccgc gattcaggcg
     5041 actattgaac tggcacaaaa accgatagtg ctgattggcg gttgtaccgg cagcggtaaa
     5101 acgctgttag tgcagcaaca gccgaacggt gttgatctgg aagggttggc gcgtcatcgc
     5161 ggttcggcgt ttggtcgcac gttacaacca caacttagcc aggcgagttt tgaaaacctg
     5221 ctggctgccg aaatgctaaa aaccgacgcc cgtcagaatt tgcgcctgtg ggtgctggaa
     5281 gacgaaagcc ggatgatcgg ttcgaatcac ctgccggaat gcctgcgcga gcgaatgact
     5341 caggcggcga ttgcggtggt agaagatccg tttgagatcc gtcttgagcg cctgaacgaa
     5401 gagtatttct tgcgtatgca tcatgatttt acccacgcgt acggcgacga acagggctgg
     5461 caggaatatt gcgaatacct gcatcacgga ctttcggcta ttaagcgtcg gctggggcta
     5521 cagcgatata acgaactggc tgcaaggctg gatgcagcac tgacaacgca actcaccacc
     5581 ggcagcaccg acggtcatct ggcctggctg gtgccgttac ttgaagaata ttacgacccg
     5641 atgtatcgct atcagcttga gaaaaaagcg gaaaaagttg tctttcgcgg tgagtgggcg
     5701 gaagtggcgg aatgggttaa ggcgcagtaa tgagggacgt atttgtatta gtcgcgactt
     5761 gatctgacac atggccttga aaggttgaga gttaccggtt ttgatatgag tgtcgaatcc
     5821 ttatccaaaa caagaggtaa ctctcatgct tcatactgcc aatcccgtca tcaaacacaa
     5881 agccggtttg ctcaatctgg ctgaagaact cagcaacgta tcaaaagcct gtaaaatcat
     5941 gggcgtttcg cgtgatacgt tttatcgtta ccgcgaactg gtcgctgaag gcggtgtgga
     6001 tgcgcagatt aatcgtagtc gccgcgctcc taaccttaag aaccgtaccg atgaggcaac
     6061 tgaacaggct gttgttgatt acgccgtcgc tttcccgaca catggtcagc accgggccag
     6121 caacgaactg cgtaaacagg gggtttttat ctccgacagt ggtgtccgtt ctgtctggtt
     6181 actccataac cttgagaatc tcaaaagacg atactgaaca aattttatca ggtgacgttc
     6241 cgtaaaaagt tgtatggagt tctcgatgca gtcggcgcga ctatcagtac ccccgctgaa
     6301 ccctggacta tcgggccgcc gtactggggc atgtcaccct ggcgtgccgc tggttttccg
     6361 ctaatggttt atttcctctt tctttcccgg caagaattta ttttaatccg gaaggattat
     6421 ttattttcag atagggactc acattaaaca cgtaacattt taattaatgc atggctgaat
     6481 atgtattaca caatgtcaga tatcgtaaaa gaccagtgca attctatgta aactctctga
     6541 ctgttcattt tatttgttgt ttcagggtcg gaactcaccc tcaataaaca actgaatacg
     6601 agtagatatt catcgtctga gctatatggc tttacacaat agccgacatg tcttttctca
     6661 gtcaggaatt ttacttttcc tcactacgaa gttgacgac
//
LOCUS       DBFIFM010000224         6685 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.224, whole genome shotgun sequence.
ACCESSION   DBFIFM010000224 DBFIFM010000000
VERSION     DBFIFM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.224"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..345
                     /locus_tag="N7C74_004113"
     CDS             <1..345
                     /locus_tag="N7C74_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HGU2433876.1"
                     /translation="SVNQALTDKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVY
                     FCDPQSPWQRGTNENTNGLIRQYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTP
                     KEIIERGVALTD"
     gene            complement(353..619)
                     /locus_tag="N7C74_004114"
                     /pseudo
     CDS             complement(353..619)
                     /locus_tag="N7C74_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
     gene            777..1961
                     /locus_tag="N7C74_004115"
     CDS             777..1961
                     /locus_tag="N7C74_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HGU2433877.1"
                     /translation="MSETRFHGARVTESTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEGGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ATCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNLKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDIEDNSTTNLRQGRVRIRYKYTPVPPLEDMELYQSFTDEFFGPAFAVLG
                     GA"
     gene            1961..2473
                     /locus_tag="N7C74_004116"
     CDS             1961..2473
                     /locus_tag="N7C74_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HGU2433878.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDDESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGKDIVP
                     DRIRSALGLG"
     gene            2528..2893
                     /locus_tag="N7C74_004117"
     CDS             2528..2893
                     /locus_tag="N7C74_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2433879.1"
                     /translation="MKETKNIDTENTVVTDTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKAESMFTLLSHVTSPRLDEVTIKKMASRDFIQLCVVA
                     VNFLSGADSGGKNEQATEA"
     gene            2902..3057
                     /locus_tag="N7C74_004118"
     CDS             2902..3057
                     /locus_tag="N7C74_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HGU2433880.1"
                     /translation="MCFEHIEDFVADIAVIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            3044..5847
                     /locus_tag="N7C74_004119"
                     /pseudo
     CDS             3044..5847
                     /locus_tag="N7C74_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            5860..6348
                     /locus_tag="N7C74_004120"
     CDS             5860..6348
                     /locus_tag="N7C74_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2433881.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTGTNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LL"
     gene            6505..>6685
                     /locus_tag="N7C74_004121"
     CDS             6505..>6685
                     /locus_tag="N7C74_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HGU2433882.1"
                     /translation="MLSYLMAIHFVLFGNSTNLKNFWKHEVIRRKRMDIWRLLREKKQ
                     RNRNFLFWWRLANEMY"
BASE COUNT         1616 a         1595 c         1928 g         1546 t
ORIGIN      
        1 tcagtaaatc aggctcttac cgacaaattc ctgagtttac cgtcagaact cagaaaatca
       61 ctgacatggg acagaggaat ggaactggcc agacatctag aatttactgt cagcaccggc
      121 gttaaagttt acttctgcga tcctcagagt ccttggcagc ggggaacaaa tgagaacaca
      181 aatgggctaa ttcggcagta ctttcctaaa aagacatgtc ttgcccaata tactcaacat
      241 gaactagatc tggttgctgc tcagctaaac aacagaccga gaaagacact gaagttcaaa
      301 acaccgaaag agataattga aaggggtgtt gcattgacag attgaatcta caagtgcctt
      361 tttctgtcag cggttctcct ttccagccaa tgagaaccgt caggcgcgcg ccccgtggcg
      421 gtagctgcaa ctgaccatct gcatcatcca gcgtgatggt gagctggtcc gcctcaaatc
      481 cccggttgtc ggtcagtgac aggctcatca ggcgctctgc cacgcctgac agcgttttac
      541 cctccgcgag aatatcaaaa tccggcactt tcacgtggtc tgtgccctga ctgagcaatt
      601 gcatggtggt gtcggtcatc tgttccctcc ctgtgcggca tggtcgcatg tgcgtgcgga
      661 gggggttact gctttttgtt gtcgccgtgg cgggagaacg gcgcaggggt gagattacgc
      721 gcgtggtggg tgatgattgt tgccgaatca tttaacggat acaaggggct gaagctatga
      781 gtgaaactcg ttttcatggt gcccgtgtta cggaaagtac cgacctggta acagcgatta
      841 atgatgttga ttccagtgtt atcggtatcg tggcaacggc agatgatgcg gacgcggagc
      901 tgttcccgct gaacaagccc acactgctga cccgcgtcaa tgacgtgctg ggaaaatgcg
      961 ggactacggg gacgctttat cgtgcgctta aggctatcgc agaccaggtg agcacaaaag
     1021 tgatcgtcgt tcgcgtggct gaacacaaag aagaaggcgg taagacgcag gatcaactgg
     1081 ttatcggtgg ttctgaatct gacggcagct atacggggat gtatgcgctg cttgttgcag
     1141 agcaggatga aagcatcgga taccgtccgc gtattctggc cgcgccggag ctggacacgg
     1201 aggcggtaac aaaatccctg tgcgtgattg cgggtaaact gcgcgcgttt gtgtatgcca
     1261 catgtcatgg ttgtaacacg atggctgagg cgattaccta ccgccagaaa ttcaacgaac
     1321 gtgaggtgat gctcttatgg cctgacttca tcgcctacaa cctgaaaagt ggcaaaaacg
     1381 aaacgttccc cgcgcctgct tatgcgtgcg gccttcgtgc gtacattgac catgagcagg
     1441 gctggcacaa atcgctgtcc aacgttccgg ttaaaaatgt gcttgggatg tcgaggcatg
     1501 tgttctggtc gttgcaggcc gaagacagtg atgccaacag cctcaacaac aaagaaatca
     1561 cgaccattat tcgtcgcaac gggttccgct tctggggcaa ccgcacaccg gaaacgaacg
     1621 cctacatctt tgaggtgtat acccgaaccg cacaggtgct ggctgattca attgcggaag
     1681 cgcagtttga aaccatcgac agtccactga cacctgcgaa cgtgaaggat gttatcagtg
     1741 ccatcagggc aaaactggat tcactggtta ctgccgggaa actgattggc gcggagtgct
     1801 ggtatgacat cgaggataac agcaccacga atttacgtca ggggcgtgtg cgtattcgct
     1861 acaaatatac gcccgttcct ccgctggaag acatggagct ttaccagtcg tttactgatg
     1921 aattctttgg tcccgcattt gcggtgctgg gaggtgcctg atggctgtac caaaacatct
     1981 tcgctttttt acgctgtttg tggatggtga aaacgaagtg ggtaaggtga cgtccgtcac
     2041 cctgcctaaa ctgacgcgca aaaccgacag ctaccggggt ggtggcatga tgggtgcggt
     2101 aagtattgac ctcggcctgg acgactccgc gcttgatgcg agctttgtca tggggggcgc
     2161 agttcgtgag ctgttcctga agtatggcgg cacgattgac ggcacgctac tgcgttttgc
     2221 gggggaatac tacaccgatg atgaaagcga cctgtatgaa gtcgaaatgc gcggacgtgt
     2281 gacggaaatt gatatggggg aagccaaaca gggcgaagcc acatcacaca cttacgccat
     2341 taaaaacacc tactacaagc tgagtgttaa cgatcgcccg ttgtgggaga ttgacctgct
     2401 gaacttcatt taccggaagg acggcaagga cattgtgccc gatcgcatcc gttccgcgct
     2461 tgggcttggc tgataagtaa tatgcaggcg gcgcagtgcg tcgcctctga ctgaaaggag
     2521 tttcctgatg aaagagacga aaaacatcga taccgaaaac acggtagtta ctgacactgt
     2581 gaaagaaacc agtgagcgtg gtgtaaaact tacccaacca attgagcgag gcggcgaaaa
     2641 aatcacgtat gtggagatca ccggggctat tgagcaggct ggatctctgc gagatttgtc
     2701 gctgtctgat gtgctgaatc tgaaagcgga atccatgttt acgctgctgt cacacgtgac
     2761 atcaccgcga ctggatgaag tgacgatcaa aaaaatggca tcccgtgact ttattcagtt
     2821 atgtgtggtt gccgtaaatt ttttgagcgg tgcggactct ggcgggaaga acgaacaggc
     2881 gacggaagcc tgatcacggt tgtgtgcttt gagcacatag aagactttgt ggcagatatt
     2941 gccgttattt ttaactggtc gcccgccgaa atcttcatga tgacgcccgg cgaagtggtt
     3001 agctggcgtg agcgggcggc acttcgcagc gggaatgcag acaatgaaga ctcttgatat
     3061 ccgggtcgct ttcagcgccg ttgacaggct gacccgacct gccgaaaacg cacgccgcct
     3121 gatggggcag tttggtgact ccatccagcg aacgcagggg gcgatcaaaa atctcgagcg
     3181 tcaggcgcga tcatttgagc gcgcccgcga cgctgtcagt aaagcggatg ctggcatcgt
     3241 gaaagcacga cgccagctta acgcccttaa tcagttacaa cgcacgggta cagtgctcag
     3301 cgaaaaacaa caaaagctga tgcagcagtt aagcacccgg cttgaacgcc tgaatgaatc
     3361 gcgcacacgg gaaattcaga aaatgaggga acttggcgga gagctgaaac gccacggcat
     3421 ttccctgaca ggcagcgata acaccattca gcaggccatc agacgcaccg aacaatacaa
     3481 caaccagctt gaacgcgaac ggcaggcgct tgcgcgtgta acgcaggcgc gtgagcggta
     3541 ttcgcacgcg caggaaaccg cgggaaaact gaaaacaggt ggtgcgctgg caattggtgc
     3601 ggcagcggcg ggcggctatg ctgccgggcg ttttttgcag cctgcgatcg ggttcggcaa
     3661 agagatgtcc cgcgttcagg cactgacgcg aatcgacaaa aacagcccgc agtttaaggc
     3721 gctgcgtgag caggcgttaa aacttgctga aacacagttt actgcgagtg atgccgccag
     3781 tgggcagagc tttctggcaa tggctggttt tactccgcag gccattcagg ccgcattgcc
     3841 cggtgttctt aatatggcgc tggcaggtgg cgtcgaactc ggcgagacgg ctgatatagg
     3901 ctccaatatc ctcacacagt tcaacctgac agccgatcaa atggaccggg tgggcgatac
     3961 gctgacagca gcatttaccc ggaccaatac tgatttacgc gcgctgggcg aaaccatgaa
     4021 gtataccggt ccggttgccg caaaacttgg tatcagtctt gaagaagcgg cggccatggc
     4081 cgggatgctt gccaataatg gtcttcgcgg aagcgatgct ggcacggcca tgcgcgcaag
     4141 tctgtcccgc cttgcatcac cgccaaaagc tgcggctgat gcactgaaag agctgggggt
     4201 gtcagttgct gacgccagag gcaaaatgcg cccgatggag gatgtgctgc ttgatctcta
     4261 taaggcgaca caaaaatacg gacaggtgga ccaggtctcc ttcttcaagg acatcgccgg
     4321 agaagaggcg ttcgttggtt tgcagacgct tgttgcggcg gctggttcag gagagctgca
     4381 aaaactgacc agagaattgc agggggcaag gggagaggcc gatcgcgtcg caaaagtaat
     4441 ggctgataat cttgatgggg acctgaaaaa tctcgacagc gcatgggaag gtcttcgtat
     4501 tcgcatcagt gatctggttg acggtccgct gcgttctgtc acgcagtggc tcacgcgggt
     4561 gcttgaaaaa atcacctcgc tggcgcaggc ccatccggta ctgacgcgcc agctactgat
     4621 agcaggcggt gcgttgctgg caatgactgc aacgattggc tcgttgtcgc tggttattgg
     4681 ggtgctttac gggaagctgg ccaccctgcg tcttggtttt gacattctta cccggtcaat
     4741 gaatgtcgtc agggtgttac ctgcgctatg gggaatggtg acgggttccg tttctttact
     4801 gggaggcgtt atcggggcgt tgttcagtcc ggttggtctt atcgtggctg cgcttgccgg
     4861 agctgccgtt cttatctgga aatactggga tcctatcagg gctttctttg ccggggtgtt
     4921 cagcgggatt atggaaaggc tgaccccgtt gcgcgacacc tttgaacggt ttggccctgt
     4981 ttttgacgta atcggaagcg gttcagccag gtgtttaact ggtttaaatc gctgctgtca
     5041 ccgatggagt ccagcaagga aacgctggat aaatgtacca gtgctggcga ggtattcggt
     5101 aacgttcttg gtggtgcgtt acagcttgtt ctgacgcctg caaaaatgtt gctggatacg
     5161 ctggcgtgga tacttgaaaa actcggtgtg cttccggatg aagcggaaag ggcgcgcaag
     5221 aaaatcgaag acgcacaacg tgcggccatt cttcaggaca aggttgctct gtttcaggga
     5281 gacattgcga aaatcaatcc gccgaagtct gcgggaaata gcaatggcac cggagatgat
     5341 aaacccaaag acaacaaacc tctcacagac agcaataccg gcacgctacg cagactcagc
     5401 aaaattgctg ataacacagg taagctggtt gatgagacaa aaaaacgcat tggccccggc
     5461 gatattgtct ttaagaacct gccccgcgca cttgctgttc gtggggagtg gcaggagcgg
     5521 aagattgcgc aggtcagtaa gcctgccccc gcaattaata tcacacccgt ggtcccggct
     5581 ccgctgcctc cggcgctggt ccctgttgtt gcggccagct cccgcccggt ggtggaggcc
     5641 atacgatcgc cagtggcatc agttcctgca acttcccgta accgggagcc tgttgcctcc
     5701 ggatttggcg gtgaaattca tgttcatctg cataacgttg ttacgcagaa tccccgcgaa
     5761 ctggcgaaac tggtcggcga aatggtcagg gcagaaatgg aacggcgcgc ccgtgccagg
     5821 cgtggcagtt tttacgataa agattgagga gtcatggcca tgatgatgat ctacggcatg
     5881 tttgtttttg agctgcgcac gttgccgcat cagcagttac agcaaaacaa aagctggcga
     5941 catgtgaaaa atgaacgcgt taaccgttca gcaagctggc agtatatcgg tgcaggtgat
     6001 gatcgcatcg ttctttctgg tgttctttat cctgagatta ctggtggcga agtgtcgctg
     6061 tcgttgttga ccacgcaggc atatacagga cgtccttggc ctttgattga tggcgtcggg
     6121 cagatttacg gcatgtatgt cctgaccgga acgaatacga cccgctccga gtttgatcgc
     6181 tacggtaagg cgaaaaagat agaattttca ctgactcttg aacgctgtga tgaggatttg
     6241 cgggagcgcc tgcaatcctc atcgttcagc gatatgctgt ccggcttcaa agataaggtg
     6301 acatcatccc ttaatagcgc ggccagctca gttaaggggc tgctctgatt taacgtatgt
     6361 cgccaatttc ctgatgaagg tgactggcga cttgctgttg tatgtccttc tcagaaaatt
     6421 gtttttgaat aacaaataac aggattttat aatctcttaa ccttataaca tgtgtggtct
     6481 gaaataataa ttaaggagat tatcgtgcta tcttacttaa tggcaattca ctttgtttta
     6541 tttggaaact ctactaattt aaaaaacttc tggaaacatg aagtaattcg gcggaaacgt
     6601 atggatatct ggaggctttt aagagagaaa aagcagcgta accggaattt ccttttctgg
     6661 tggcggttgg ctaacgaaat gtata
//
LOCUS       DBFIFM010000225         6501 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.225, whole genome shotgun sequence.
ACCESSION   DBFIFM010000225 DBFIFM010000000
VERSION     DBFIFM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.225"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..192)
                     /locus_tag="N7C74_004122"
     CDS             complement(<1..192)
                     /locus_tag="N7C74_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433883.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNYKLAKRIYFITFIY"
     gene            270..1364
                     /gene="ddlA"
                     /locus_tag="N7C74_004123"
     CDS             270..1364
                     /gene="ddlA"
                     /locus_tag="N7C74_004123"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGU2433884.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1388..1600)
                     /locus_tag="N7C74_004124"
     CDS             complement(1388..1600)
                     /locus_tag="N7C74_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HGU2433885.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1860..2168
                     /locus_tag="N7C74_004125"
     CDS             1860..2168
                     /locus_tag="N7C74_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HGU2433886.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2227..3321)
                     /gene="yaiW"
                     /locus_tag="N7C74_004126"
     CDS             complement(2227..3321)
                     /gene="yaiW"
                     /locus_tag="N7C74_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HGU2433887.1"
                     /translation="MSRVNHLSSLSFLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSGIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3334..4554)
                     /gene="sbmA"
                     /locus_tag="N7C74_004127"
     CDS             complement(3334..4554)
                     /gene="sbmA"
                     /locus_tag="N7C74_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HGU2433888.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4906..6063
                     /gene="ampH"
                     /locus_tag="N7C74_004128"
     CDS             4906..6063
                     /gene="ampH"
                     /locus_tag="N7C74_004128"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HGU2433889.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6064..>6501)
                     /locus_tag="N7C74_004129"
     CDS             complement(6064..>6501)
                     /locus_tag="N7C74_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2433890.1"
                     /translation="GITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQTITLI
                     EQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRIGVMN
                     YIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
BASE COUNT         1642 a         1670 c         1578 g         1611 t
ORIGIN      
        1 ataaataaaa gttatgaaat atattctctt cgctaactta taattaagag aaaaaaactc
       61 accgcatggc catttaagcc aggaataaat tagcttcccg ttcatcattt ttgtgatctt
      121 gcgcacatct ttgagataac gcactttctt caccagtacg ttgcacgctt tgtcagcaga
      181 agattgcaac aatctttcag aaggccgcga atcccccgtt ttgtcgtggt aatgtatgca
      241 ctttgtgtta gacgggatag gtttttaaga tggaaaaact gcgggtagga atcgtttttg
      301 gtggtaaatc agcggaacat gaagtgtctc tgcaatcggc aaaaaacatt gtcgatgcca
      361 ttgataaaag tcgcttcgac gttgtgctgc tgggcattga taaacaaggg caatggcacg
      421 tcagcgatgc cagcaattat ctgctaaatg cagacgatcc tgcccatatt gcgttgcgcc
      481 cttcggcgac cagcctcgcc caggtgccag gtaaacatga gcatcagctt atcgacgcgc
      541 aaaacggtca gccgttgccg acggtggatg tcattttccc gattgtccac ggtacgctgg
      601 gcgaagatgg ttccttgcag ggaatgctgc gggtcgccaa tttaccgttt gtaggttctg
      661 atgttctggc ttcagcagcc tgtatggata aagatgtcac caaacgtctg ctacgcgatg
      721 ccgggctgaa cattgcgcca tttattaccc tgacgcgcgc taatcgtcac aacatcagtt
      781 ttgccgaagt ggagtctaaa ctggggttac cactgtttgt aaaaccggct aatcagggct
      841 cttctgttgg tgtcagcaaa gtaaccagtg aagaacaata cgcaattgcc gtcgatctgg
      901 cgttcgagtt cgatcataaa gtgatcgttg agcaagggat caaaggtcgt gagatcgaat
      961 gcgcagttct gggcaacgac aatccgcaag ccagcacctg tggcgagatc gtactcacca
     1021 gcgatttcta tgcctacgac accaagtaca ttgacgaaga tggcgcgaaa gtggtagttc
     1081 cggcagccat tgcgccagaa atcaacgata agatccgggc gattgccgtt caggcttatc
     1141 agacgttggg atgcgcaggc atggcgcgtg tagacgtgtt tttaacccca gagaacgaag
     1201 tggtgatcaa cgagatcaac acactgcctg gcttcaccaa catcagtatg tatccgaagc
     1261 tgtggcaagc cagcggtctg ggttacaccg atctgatcac acgtttgatt gagctggcgc
     1321 tggagcgtca cgctgcggat aacgcattga aaaccacaat gtaatatttt aacgcccgat
     1381 aatattctta ttgggcgttt tcagtttctt catcgcgacg acgaataatc aaccccgcca
     1441 gccagaaact aatcacccac gtcaccagac cgacggcata ggtctgccaa ccttttgctt
     1501 caaatcccag taaccccacc acgccattaa gaatgaatat aaggccaatg gcgaacgcat
     1561 agtagtgcca gtcacggcgg atttttacag gcagattcat ggctgctccg ttaacgaaaa
     1621 acaacatcct tgcatattct gcaacaaccg tctgtggtgt agacaacatt ttcgcaattg
     1681 ttaataaatt taacttaaag ttgctatttt gtgactcgca acgaagattc acgcttcggg
     1741 aatattccca acatcaattt accatgattc tgatattgac aatctaaatg acctgtcaga
     1801 cttgccataa gccgctggta tcacaccaga agagaagcag atgacttccg gaggttttta
     1861 tggctgattt caccctgtca aaatcgctgt ttagcggaaa atatcgcaat gcctcttcaa
     1921 cgcctggcaa cattgcctat gcgttgtttg tgctgttttg cttttgggct ggggcgcaat
     1981 tgctgaacct gttagtgcat gcgcccggcg tctatgagcg tttaatgcag gtccaggaaa
     2041 caggtcgccc acgggtggaa attggtttag gtgtcggcac cattttcggg ctgatcccgt
     2101 ttttagtagg ctgcctcatt tttgcagtgg tggcgctatg gctgcactgg cgacatcgcc
     2161 gccagtaaga tttacgttgc cagatacggc ctaaaacgcc ttatctggca tgacattcct
     2221 gtttgttcac tgtttcatac aacgcgcccg ccgttcgtct acgcgcttcg caaaccaggc
     2281 cgtagtcagg ttgcgggtga ttttcgggct ttccagttga atgccaggca acatttcgcg
     2341 ggggagtgat ttacccgttt tcgcttcggc aagttgataa actttcttgt acagcgccgt
     2401 ctcctcaaaa gagaagctgt cacctttctc tagctgacga cgaatttcgc tgtcgctcat
     2461 ccccagtttt gccgccagtt tgcgcgttgc cagttcggtt ttcccgggtt ctttactgtc
     2521 gtaacgaatt aaatcaccgt ccagtgccag cttcacgccg ctggccttac tgaccgcgtt
     2581 ctgaaacgcg gcattacggc tggcgtacca gccagcgtta aaatcagcaa aacggtatat
     2641 cggtgcgcta taactggcgg gataattcag taaatggtaa gtaccaaacc acaacccgcc
     2701 gcggcgacta aagacttcct gacggactgt accgtccatt ttccacggat accctttggt
     2761 atgctgttcg gcaaaagcaa tgctgacctg catcggccca ccggtgcgca ccggattaag
     2821 cgaaccaaac aacgtctgcc ccattggcac catgctgatc agatcgtcaa aaatcccgct
     2881 caattgcttt tctgtacgca ccgaatctaa ccgttcgcta taacttttgc cgtttggcga
     2941 tttgattttt agcgcagtat gaaccagaaa agcaggaatg tgcatccgtt cagcacgacg
     3001 gtcaatctct tgccaggcga tcttgcttaa gcccggaacg gccggatctg cctgataatt
     3061 tgactcctgc tgcgccaccg ccagcaccga gcagacgttt tccagcgtcg gtgccagccc
     3121 ctgactttca aaggtggtgg cgagatcttt cgcccaggca tcgcggtctt tcacgctcgc
     3181 aggcatcttc tgccgcacca cgctagccac atctatcgct ttttcgccct ttttcaacgg
     3241 ctgtggcgcc tgactgctac accccgctaa aaccagaacc gccaaaaatg aaagcgaaga
     3301 cagatgattg actcgcgaca ttacttctcc tttttagctc aaggtatggg ttacttcctg
     3361 aattttgtca ccatccagct catgttcaaa gctgcgcaga cgtttgtaga tagacatcaa
     3421 ctcaaccagt gtggtccatg agttaatcag gtactggaaa gcaccgcgaa cctgaccaaa
     3481 aacgttggta atctgcgtca tcaggccgag cgtaatcgta ccggcaacaa ttgacggaaa
     3541 cagcaagaac aaaccgaaaa cgttatcgac ctgcaaatag agaatgcgag cgatgttgaa
     3601 atacatatag tgaaaataga ggcggaaata gtttttccgt acggcgctaa acagctcgcg
     3661 taccgttggc ggcgtcgcgc gcgtggcatc gtcttcacca taaaccagct ctttacggta
     3721 ggcagcctct acacgctggt ttttaaactc cagccccggc agtttgatcc ctaccactgc
     3781 cagcaatccg gtccccatca gcgaccagac gatagcggca atcaccagac catacggaat
     3841 gtgcccgata atcggcagct ccggcacatg cgcggagagc gttaccaaca ccggcaggaa
     3901 ggcgatcaac gtcatgatgg cgttgataaa actgacaccc atattctcca gcgttgaagc
     3961 aaaacgcatg gtgtcttcct gcacacgctg tgcggcgcct tcgatatgac gcagttgttg
     4021 ccagttcgcc atgtaatatt cgttcatcgc cgtacgccag cggaacacgt agtgactgac
     4081 aaagaagtta ttcagcacac tgatcactac cgcgatcagc gcaatcccca gaaagacgcc
     4141 cacttcgcgg taaaattgtt cgatagtgac tttatgcggc gaacttagcg cggtttgaat
     4201 cagatcatag aacggtgcat accaggcgtt gacggcaacc ccaacttcca ccaaaaacca
     4261 ggtgacgaag atgatcagtg cagtaccgag tattgaccag tattgccaac ggtgcgggct
     4321 gtagataaac cagaacaatg caaaaagtcc tacgcaaaca atgtagtaag cgtaaaaaat
     4381 caggaaatcc aacgaccaga aacgcgcggc gctaatcggg atctgcccgg aagcgccggt
     4441 gatacgcgcc acccagtccc ccccaccggc ttgccagaag ataacggcaa tcaatgccca
     4501 aacaaaggcc gagagaaaaa acgttcccgg ctttgggaaa aaagacttaa acatatgcac
     4561 tcctgctaac ttcttatcgt tttaacaact gctgtgtatt aaccgcatga ccgctacaaa
     4621 gcgtcagcca aatgaaaccg cccattgtga ctcatagcaa ggcgcttagt tcgccagcct
     4681 ggccataaga attgtttcga cggatttcac aaaccaaaga ttgtttacat aagcggatta
     4741 acacgcgcag gatcacaaat gcaggcattg gtgagcagga aaaagatcgt gtttacgcgc
     4801 cgttacgttt ttaacactat tattgcacta attcgccctt tgcaatccat caatgagtag
     4861 tataaatacg ctcagttacc ttcattcaat ttatggacac caccgttgaa acgtagtctg
     4921 cttttttctg ccgtgctgtg tgcggcgtca ttgacctccg tccatgcggc gcagccgatc
     4981 actgaaccgg agtttgcctc tgatattgtc gatcgttatg ccgatcatat tttttacggc
     5041 agcggtgcca cggggatggc gctggttgtt atcgacggta accagcgcgt ctttcgcagt
     5101 tatggcgaaa cgcgacctgg taataacgtt cgcccgcagc tggattcggt cgtgcgtatt
     5161 gcttccctca ccaagctaat gaccagtgag atgctggtga aattgctcga ccaggggacc
     5221 gtgaagttaa acgatccgct aagtaaatat gctccgccag gcgcacgcgt gccaacctac
     5281 aatgggacgc ccattacgct ggtcaatctg gcaacccata ccagcgccct gccccgtgaa
     5341 cagcccggtg gcgcggcaca tcgtccggtg tttgtctggc caacgcgcga gcaacgctgg
     5401 aaataccttt ctacggcgaa gctgaaagcc gcgccaggca gtcaggcggc gtactctaac
     5461 cttgcgttcg acctgctggc agatgcactg gcgaatgcgt ccggcaagcc ttatacccag
     5521 ttgtttgaag agcagattac ccgtccattg gggatgaaag acaccaccta cactccctca
     5581 ccggatcagt gccgccgttt aatggttgct gagcgcggtg ccagtccgtg caataacacg
     5641 ctggcggcaa ttggcagcgg cggcgtctat tccacgcctg gcgatatgat gcgctggatg
     5701 cagcagtatt tgtcatctga tttttatcag cgcagtaacc aggcagatcg catgcagacg
     5761 ttgatttatc agcgcgcgca gtttacgaaa gtgattggca tggatgtccc cggcaaagcg
     5821 gatgcgcttg gtttaggctg ggtatatatg gccccgaaag agggtcgtcc ggggattatt
     5881 cagaagacag gtggtggtgg cggtttcatt acctatatgg cgatgatccc gcagaagaat
     5941 attggcgcgt ttgtggtggt cactcgttcg ccgttgacgc gctttaaaaa tatgagcgat
     6001 ggcatcaatg atctggtgac cgagctaagc gggaataaac cgttggttat ccccgcatcc
     6061 tgattaatac tctgaaggca aacggttgat agcgaccagt ttgcctttat tcatttcgat
     6121 atagccgcct ttacgcaaag cagcgagaac ttctgcgacg acagaacgcg atatgcgtgt
     6181 acgttgatgg atataattca tcacaccaat acgtgatcgc aattcttcat tccagtcaat
     6241 cattgataat aatgttgcgc ggatttgttc gtaggaatta tgcccaatga gctgcacgtc
     6301 gcgtaattcc agaattctat tttgccaggc taaccagtaa aaagcgtctc gccagagttg
     6361 attttgttca ataagcgtaa tggtttgttt ggctggtaga tgatatcccg tacaatttcc
     6421 ttctgatatt aatttgtatg gtatatcgtt tttcattaaa ccatcagcca gccccataat
     6481 ataaggagcc tgggtaatac c
//
LOCUS       DBFIFM010000226         6461 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.226, whole genome shotgun sequence.
ACCESSION   DBFIFM010000226 DBFIFM010000000
VERSION     DBFIFM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.226"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            153..278
                     /locus_tag="N7C74_004130"
                     /pseudo
     CDS             153..278
                     /locus_tag="N7C74_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
     gene            343..603
                     /locus_tag="N7C74_004131"
                     /pseudo
     CDS             343..603
                     /locus_tag="N7C74_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            653..892
                     /locus_tag="N7C74_004132"
                     /pseudo
     CDS             653..892
                     /locus_tag="N7C74_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(940..1557)
                     /gene="ureG"
                     /locus_tag="N7C74_004133"
     CDS             complement(940..1557)
                     /gene="ureG"
                     /locus_tag="N7C74_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HGU2433891.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLELIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(1569..2243)
                     /locus_tag="N7C74_004134"
     CDS             complement(1569..2243)
                     /locus_tag="N7C74_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HGU2433892.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(2244..2708)
                     /gene="ureE"
                     /locus_tag="N7C74_004135"
     CDS             complement(2244..2708)
                     /gene="ureE"
                     /locus_tag="N7C74_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HGU2433893.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(2718..4421)
                     /gene="ureC"
                     /locus_tag="N7C74_004136"
     CDS             complement(2718..4421)
                     /gene="ureC"
                     /locus_tag="N7C74_004136"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HGU2433894.1"
                     /translation="MSNISRQAYADMFGPTTGDKIRLADTELWIEVEDDLTTYGEEVK
                     FGGGKVIRDGMGQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNP
                     DIQPNVTIPIGVSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTG
                     PTAGSNATTCTPGPWYIYQMLQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKI
                     HEDWGATPAVINCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGA
                     GGGHAPDIITACAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAE
                     SRIRQETIAAEDVLHDLGAFSLTSSDSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEES
                     GDNDNVRVKRYIAKYTINPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKG
                     GMIAMAPMGDINGSIPTPQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLH
                     STTAVVKGCRTVQKADMRHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFL
                     F"
     gene            complement(4414..4734)
                     /locus_tag="N7C74_004137"
     CDS             complement(4414..4734)
                     /locus_tag="N7C74_004137"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HGU2433895.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVELVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(4743..5045)
                     /locus_tag="N7C74_004138"
     CDS             complement(4743..5045)
                     /locus_tag="N7C74_004138"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HGU2433896.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(5136..5834)
                     /locus_tag="N7C74_004139"
     CDS             complement(5136..5834)
                     /locus_tag="N7C74_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HGU2433897.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGLLTVQRPFYPEEETCHLCLLHP
                     PGGIVGGDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYLGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQ"
     gene            6215..>6461
                     /locus_tag="N7C74_004140"
     CDS             6215..>6461
                     /locus_tag="N7C74_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433898.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGGPPEMNGI"
BASE COUNT         1503 a         1666 c         1719 g         1573 t
ORIGIN      
        1 aaacttctta tgaaataaac gtttacccga cgtatctgat cccgctgttt ttacctgtat
       61 cctttaacat acttgagaag cccgctgaaa aaatataccg taaaggcggt ttctctgtct
      121 tccataaacc tgtcggataa gaagggcatt aaatgaaaac caaaaaagtt atgatgattg
      181 cactggtcag ctctgcgctg gctctttcag gatgtggagc catgggcaca gcgatcaaaa
      241 aacgaaatct ggaagtaaaa acccagatga gtgaaacctg aggtagcctg agtttaacgg
      301 acactccttc ctgaaataga atggcatcag aaggagctaa taatgagcag aaaaacccaa
      361 cgttactcta aagagttcaa agccgaagct gtcagaacgg ttcttgaaaa tcaactttcg
      421 atcagtgaag gcgcttcccg attatctctt cctgaaggca ctttaggaca atgggttacc
      481 gccgccagaa aagggctcgg tactcctggt tcccgcacgg tggctgaact ggaatctgaa
      541 attctgcaac tgcgtaaggc gttaaatgaa gctcgccttg agcgagatat attaaaaaaa
      601 gcagctgtag attcaatctg tcaatgcaac acccctttca attatctctt tcactgcccg
      661 cgacggtatc ggttatcagt ttgacctgct ggctaacggt gaaaccgtac tgcgtgatgc
      721 atccggcgaa aaacgcatca agggtgaaaa agacggtcgt atgctcatga atgttggcct
      781 gaatgcggaa attcgcgaca acatccgctt cggtctggag tttgaaaaat cggcatctgg
      841 taagtacaac gtggataacg cggtgaacgc caacttccgt tattccttct gataaaaaca
      901 aggggccgcc tgccggccct gatacagagg atgttgtgat tacttaccca gcatgccctg
      961 ctcttcgata aaggcgatga tgttttgcag accttcacca cttttcaggt tagtgaaccc
     1021 ccatgggcgt tctccgcgca tacgtaaagt atcacgctcc attacctcga gggaggcgcc
     1081 cacgtatggt gcaaggtcag ttttattgat cacgaggaag tcggatttgg tgatccccgg
     1141 accgccttta cgcgggatct tttctccttc tgccacatct atgacatata tggtaaggtc
     1201 cgccagttcg gggctgaagg tcgcactcag gttgtcgccg ccgctttcca caaagatcag
     1261 ctcgagatta ccaaatttct cactcagtgc ttccacggct gccagattca ttgatgcgtc
     1321 ttcacgaatg gctgtatgag ggcagccccc cgtttcgaca ccgacaatgc gttccgcttc
     1381 cagtgctccg gcctcagtga gaatgcgttg atcttctctg gtgtagatgt cgttggtgac
     1441 tacggctaat tgccaggtgt cacgcatgga tttgcagagg gcttccagaa gcgcggtttt
     1501 acctgagccg accggaccgc cgacgccgac acgcagcggt tgcttgatta tgttcatttt
     1561 attttctcct aagaacggaa taatcgggaa tactgggttt catgtcgcgc ggaagcgata
     1621 gatgcaagtg gcgttgctga accaattgcg tgatccgggg ttgcaagggc actgtccatg
     1681 ttcgccgcat agcgggcaca caggcgcata attaactgct gggcagccag ctggccgtag
     1741 ggtaccagcc tgacgcccgc catcactgcg ctttcaatcc agctgtagcc aaggctcagg
     1801 gccaggtctg aaacggatat ttgccagcgt acgccgagcc atgccatacc ggttaactgg
     1861 ctttgctggc atagtttgcg ccattcggcc gggcagtcgg gctgccagtc gaccagaagt
     1921 ctggtgaagg ctgcaccgcg gttacgttct tcatcgcgta gctcacgggt ttccctgcag
     1981 gccagcagat aagcggtcca gcgcccggca ctgtcaggat cgcccgcttc acaggcccgg
     2041 tatagccggg caagcagtgg caggtcgacg gcaaaaaaac tctgttccat ctgcaactgc
     2101 tgccagtgtt caaaggcggc gctatcttct acccagcctg cttccactgc ccactccagt
     2161 ccctgggacc agctgtagcc tccaacaggg aggctgttac tggcaagctg catcagccgc
     2221 agacgttttt ccggagtcgg cattcagtgg ctgtgctcct gatcgtgatt atgggcgtgc
     2281 gacttgctgg cgtaagcgcc ggcttccggc tcaaacggaa gatgtgcaaa atcaacatcc
     2341 agcccaaact ggcgcagcat atcatcgagg acgtgatcgt gatggtagcg cagctctccg
     2401 ggcattattt gcagcggaac gtggcgattg ccgagatgcc agcaggcttt cgcgagcata
     2461 aaaggatctg cgcagcgcac tacagaaacg gcttcatcag cggcgatcac cttaatgaat
     2521 tcatcaccgt tttcgttact gagaatatca ccatcgcgca gcagcagacc gcgtggcagc
     2581 aacagtccag cctgacgtcc atcgttaagg gtgactttaa tacggctttt tacccgcatg
     2641 tcaacaggga gggtaacgct ggcagttgtc tgagccgggg tctccacccg ccgggtcaaa
     2701 taaagcattt gtgctcctta aaacaggaaa taacgttgcg ccattggcag aatgtccgcc
     2761 ggttcactgg ttatcagttc gccgttgata cgcacttcgt aggtttgtga atccacggtt
     2821 atatcaggca acaggctgtt gtggcgcata tcggcttttt gtactgtgcg gcagcctttt
     2881 accacagcag ttgtgctgtg caggttaagc tgttcagcga cgccatttgc tgctgctgcc
     2941 tgcgacagga aagtcacacg acagcggtga cgggcactgc ccaatgcagc gaacattggg
     3001 cgatagtgca ccggctgcgg tgtggggata gagccgttga tatcacccat cggcgccatg
     3061 gctatcattc cgcctttgac gatagtcgcc ggttttacgc caaagaacgc cggggaccag
     3121 agcaccagat ccgccagttt tcccacttca atcgagccga cttcatgagc aataccgtgg
     3181 gttaatgccg gattaatggt gtatttagcg atatagcgct tcacgcggac gttgtcgtta
     3241 tcaccacttt cttccggtaa cgggccgcgc tgaactttca tccggtgtgc cacctgccag
     3301 gttcgtaaca ctacttctcc gacgcgtccc atggcctgcg aatcggacga ggtgagggag
     3361 aacgcgccaa ggtcgtgcag gacgtcttcc gcggcaatgg tttcctggcg aatgcgcgat
     3421 tcggcaaagg ctacgtcctc ggcgatatcc gggtcaagat gatggcaaac catcagcatg
     3481 tccagatgct catcaatagt gttgacggta tagggtagcg tcggattggt tgaggaaggc
     3541 agaatattgg ggtgcgcgca ggcggtgata atatccggag catgaccacc acctgcacct
     3601 tctgtatgga aggtatggat agtgcgcccg ccgatggcag tcagagtatc ctcaacgaat
     3661 cctgattcgt taagcgtatc actgtgtagc gcaacctgaa cgtccatttc gtcggctaca
     3721 gtcagtgcgc agttgattac cgcaggtgta gctccccagt cttcgtgaat tttgaggccg
     3781 ataaccccgg ccgcgacctg ctcacgaagc gcatccggat tggagcaatt gcctttaccc
     3841 agcaacccga tattgaccgg caggctgtct gcagcctgca gcatttgata aatgtaccat
     3901 ggtcctgggg tacaggttgt ggcgttagaa cccgctgtcg ggccagtacc gccaccgatc
     3961 atggtggtaa tgccggatgt cagcgcttct tcagcctgct gtgggcagat ccagtgaata
     4021 tgcgtatcga cgccacctgc ggtaacgatc ctgccttctg cggcaataat ttccgtggat
     4081 acgccgattg ggatagtgac gttgggttgt atatcaggat taccggcttt gccgatagca
     4141 aatatccttc catctttgac gccgatatcg gctttaacga tcccccagta atcgatgatc
     4201 agggcattgg tcagcaccag atcagcgcag ccggcggaga gcatttgccc ctgtcccata
     4261 ccgtcgcgga ttactttacc gccgccgaat ttgacctctt cgccgtaggt agttaaatca
     4321 tcttcgacct cgatccacag ctcagtgtct gccagacgaa ttttatcacc ggtggtaggg
     4381 ccgaacatgt cagcataggc ctggcgtgaa atattactca tcatttgcct ccagctcacc
     4441 cattatatct ccgcggaaac cgtgcacgat acgggcaccg ctcagcgcca ccagttcaac
     4501 ctcacgcttt tggcctggtt cgaagcgcac tgcagttccg gcggcgatat tgagacgata
     4561 gcctcttgcc ttctggcgat cgaacttcag tgccgggtta acctccgcga agtgataatg
     4621 ggaaccgacc tgaattggtc ggtctccgtg attttcaacg ataatactgc aggtagcccg
     4681 gccgatgtta agctctatat agccaggttt aaccttatat tctccaggga tcatctgtta
     4741 cctcagatta tcggattatg gacggtaacc agcttggagc cgtccggaaa ggtggcttcg
     4801 acctggatat cggggatcat ttctggtatg ccttccatga cctgctcgcg actcaggaca
     4861 tgccgtcctt cttccatcag cgcagcgacg cttttgccgt cgcgagcgcc ctccattata
     4921 aaagcgctaa tcagggccac ggattcggga tagttaagtt tcaggccgcg ggccagacga
     4981 cgctctgcca gcagtgcagc ggtaaacagt aatagtttgt ctttttctct tggagtcagt
     5041 tccatagcgt tctcttaagt cagccagatc cggggaagta ccggtgattt gtcagtcagt
     5101 tgcggccgca taaattgcca gatatcacgc attacctact ggcaacgcag attatcatca
     5161 cttaaaaagc gaactgtcag tagaccatcg gtgagcgtgg ctccggcgta gttttccagt
     5221 ggtgtgagtt tttcgcgtat gtcttccagt tgcgtttcat tccccaggta gaacagcatc
     5281 gttcccaccc atggtttccg ggcaacgcag gtaagttctc cattggccac gtgcagacgc
     5341 tcaatcaaca gcggagaacc gtcgacccat atctccagac ggttagcgag tgttccgtgg
     5401 ctgaaggttt ctccgatgac cggacgtcca aggcacagaa gatcccaggc cagcagtgta
     5461 ctggaggacg acagatgaaa cacggtatgc aacgtggcgt gagcgccggg aaaaaaaatc
     5521 gaatcctgtg gcaaccactc cagcgttgcg ttattgcaca gcgtcaggtt ttgctgaagg
     5581 tgcgcctgcg cgccgctgct gcggtaaaat ttactggcac caggcatggt gatcagcgta
     5641 tggcaatcag agtcaattat tgcgttgatg atgagttcgt caccgccgac gataccaccg
     5701 ggtggatgaa gcagacagag gtggcaggtt tcttcttcgg gataaaaagg acgctgaaca
     5761 gtgagcagac cgacatgatg acgggtggtg agcgtggttt tcccgtgcag acgctgaaaa
     5821 cggagatcca gcatggcctg ccagcctttt ttattttgtg gtaacgcggt actgagcaat
     5881 cttacgcccc tgataaataa agataaaacg acagtaaaga gccggatttc cttgttatat
     5941 aaaagatgat gcctttccag tgagttttcg tctgaataat acgaatgttt gttttcgtca
     6001 tgaagcgttc cggctcccgg ggctatacat cgccagaaaa gcaatgcata ttgtcgggat
     6061 tatccagaga atcaggcaga aagatccgac aatgggctgt gattatgcca tctggctcag
     6121 aatgatatca aggtgttatg gggataactt gctgatacaa tagcagtatg acagattcat
     6181 taatgcggaa actgctcact gaaacagagt gttcatgact gagcactggt cgggtcatgc
     6241 agatgataat ttcccctttt cttggggaag cgacagtatt ttcatgcatg cctgtcttca
     6301 gtcatacggg gagagcatct gtgatacagt ccatacattt gttgcggacg tcgctcctga
     6361 gtcactgact ggtctgcaaa cagtttatct gtacacggca gggcttctgt gtgatatcgt
     6421 ttttcatatc gagggggggc ctccagagat gaatggaata a
//
LOCUS       DBFIFM010000227         6437 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.227, whole genome shotgun sequence.
ACCESSION   DBFIFM010000227 DBFIFM010000000
VERSION     DBFIFM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.227"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            94..807
                     /gene="aphA"
                     /locus_tag="N7C74_004141"
     CDS             94..807
                     /gene="aphA"
                     /locus_tag="N7C74_004141"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HGU2433899.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKNFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     SMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            918..1334
                     /locus_tag="N7C74_004142"
     CDS             918..1334
                     /locus_tag="N7C74_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HGU2433900.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            1338..1694
                     /locus_tag="N7C74_004143"
     CDS             1338..1694
                     /locus_tag="N7C74_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGU2433901.1"
                     /translation="MTISELLQYCMAKPGAEQSEHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(1729..4551)
                     /gene="uvrA"
                     /locus_tag="N7C74_004144"
     CDS             complement(1729..4551)
                     /gene="uvrA"
                     /locus_tag="N7C74_004144"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HGU2433902.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            4806..5342
                     /gene="ssb1"
                     /locus_tag="N7C74_004145"
     CDS             4806..5342
                     /gene="ssb1"
                     /locus_tag="N7C74_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HGU2433903.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            complement(5441..5722)
                     /locus_tag="N7C74_004146"
     CDS             complement(5441..5722)
                     /locus_tag="N7C74_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HGU2433904.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            6151..>6437
                     /locus_tag="N7C74_004147"
     CDS             6151..>6437
                     /locus_tag="N7C74_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HGU2433905.1"
                     /translation="MNHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINE"
BASE COUNT         1554 a         1666 c         1673 g         1544 t
ORIGIN      
        1 tcctttcccc tgtagctggc gttatggtca gatagttttt gcaacaaatc tcacaataaa
       61 aagtttcaac atactgacta tttagggaaa aatatgcgca agatcacaca ggcaatcagt
      121 gccgtttgct tattgttcgc tctaaacagt tccgctgttg ccctggcctc atctccttca
      181 ccgcttaacc ctgggactaa cgttgccagg cttgctgaac aggcaccaat tcattgggtt
      241 tcggtcgcac aaattgaaaa tagcctcgca gggcgtccgc caatggcggt ggggtttgat
      301 atcgatgaca cggtactttt ttccagtccg ggcttctggc gcggcaaaaa aaacttctcg
      361 ccagaaagcg aagattatct gaaaaaccct gtattctggg aaaaaatgaa caacggctgg
      421 gatgaattca gcattccaaa agaggtcgct cgccagctga ttgatatgca tgtacgccgt
      481 ggtgacgcga tcttctttgt gactggtcgt agcccgacga aaacagaaac ggtttcaaaa
      541 acgctggcgg ataattttca tattcctgcc accagcatga atccggtgat ctttgcgggc
      601 gataaaccag ggcaaaatac aaaatcgcaa tggctgcagg ataaaaatat ccgaattttt
      661 tatggcgatt ccgataacga tattaccgcc gcacgcgatg tcggcgctcg tggtatccgc
      721 attctgcgtg cctccaattc tacctacaaa cccttgccac aagcgggtgc gtttggtgaa
      781 gaggtgatcg tcaattcaga atactgacag agcgggagag cgtgacactc tcccgaatgc
      841 tgttttttta atcacacctt tatcctttcg ctgtcttgct gcaaactgat taagagagtt
      901 ttatcaagga gcagcacatg tggtatcaaa agacgctcac gcttagcgcc aaatctcgtg
      961 ggtttcatct ggtaacggat gaaattctga atcagctggc tgatatgccg cgcgttaaca
     1021 tcggcttact gcatctgttg ctgcaacata cctccgcctc tctgacactt aatgagaact
     1081 gcgatcccac cgtacgccac gacatggagc gttttttcct ccgcaccgtt cccgacaacg
     1141 gaaattatga gcatgactat gagggagcag acgatatgcc ttctcatatc aaatcctcaa
     1201 tgctgggaac atcgcttgta ttgccggtgc ataaagggcg tattcagacc ggcacctggc
     1261 aaggcatttg gctgggggaa catcgcatcc acggcggatc gcgtcgcatc atcgcgacac
     1321 tacaagggga gtaaaaaatg accatttcgg agttgctaca atattgcatg gcaaaaccag
     1381 gcgcagaaca gagcgagcat aacgactgga aagcgacgca gatcaaagtg gaagatgtac
     1441 tgtttgcgat ggtgaaagaa gtagaaaatc gcccagctgt ttcgctgaaa accagcccgg
     1501 agctggcgga gctgctacgt cagcagcaca gcgatgtgcg tccaagccgc catctgaata
     1561 aagcgcactg gagcaccgtg tatctcgacg gttcgctgcc ggattcgcaa atctattatc
     1621 tggtggatgc gtcttatcag caggcggtga atttactgcc ggaagaaaaa cgtaaattgc
     1681 tggtgcaact ctgaaaggaa aaggccgctc ggaaggcggc cttaacgatt acagcatcgg
     1741 cttgaggaag cgtgccgtat gcgaagcttc gcactccgcg acggtttctg gcgtaccgga
     1801 gacgaggatc tcgccgccgc cactgccgcc ttccggcccc aggtcgacaa tccagtcagc
     1861 ggttttaatc acgtcgagat tgtgctcaat caccacaatg gtgttgccct gatcgcgcag
     1921 tttatgcagt acgtcgagca gttgctgaat atcggcgaag tgcagaccgg tggtcggctc
     1981 gtcgagaata tacagcgtct gcccggtgcc gcgttttgac agctcacgcg ccagcttcac
     2041 gcgctgggct tcaccaccag aaagtgtggt tgcggactgc cccaagcgaa tgtacgtcag
     2101 accaacgtcc atcaacgttt gcagcttacg cgccagagct ggcaccgcat caaagaactc
     2161 acgcgcctct tcgatggtca tatccagcac ttcgtggatg gttttgcctt tgtacttaat
     2221 ctccagcgtt tcacggttat agcgtttacc tttgcactgg tcgcacggca cgtaaatgtc
     2281 cggcaggaag tgcatctcca ctttgatcac gccatcgccc tgacaggcct cgcagcgtcc
     2341 gccacggacg ttaaagctga aacgtcctgg cgtataacca cgcgcacggg attccggtac
     2401 gcccgcaaac agttcgcgca caggcgtaaa cacgccggta taggtcgccg ggttagaacg
     2461 cggagtacga ccaattgggc tttggtcgat atcgattact ttgtcgaaat gctccagtcc
     2521 ctgaatatcg cgatacggtg ccggttcggc gatggtcgca ccattcaact ggcgttgggc
     2581 aatcgggaac agtgtgtcgt taatcagcgt cgatttaccg gaacctgaaa cccctgtgat
     2641 gcaggtaaac agaccgactg gcagcgttag cgtcacgtct ttcaggttgt tgccgcgtgc
     2701 gccagtcagc ttaagtactt tttccggatt cgccggaacg cgtttcttcg gcacttcaat
     2761 cttgcgttta ccgctcatgt actgtccggt caacgactcc ggcaccgcca taatcgcttc
     2821 cagcggacct tccgcgacca cttcaccgcc atgtacacct gcaccaggac cgatatcgat
     2881 cacatgatca gcggcgcgaa tcgcgtcttc gtcgtgctcc accacaatca cggtattacc
     2941 gagatcgcgc agatggataa gcgtacccaa caggcgctcg ttatcgcgct ggtgcaggcc
     3001 gatagacggc tcatccagca cgtacataac gccaaccagg cccgcaccaa tctggctcgc
     3061 cagacggata cgctgggctt caccgccgga aagcgtttct gccgagcggg aaagcgtcag
     3121 gtaattcagg ccgacgttaa cgaggaattt caaacgatcg ccgatctctt taagaatttt
     3181 ttccgcaatc ttcgcccgct gacctgcgag tttgagattg ttgaagaatt ccatcgcatg
     3241 accaatgctc atgtcggaga tggcgggcag cggcgtattc tcgacataca cgtggcgcgc
     3301 ttcccgacgc agacgcgtcc cttcgcagct ggcgcacggg cgattgctga tgaacttggc
     3361 taattcttca cgtaccgcac tggattctgt ctctttataa cggcgctcca tattgtgcag
     3421 cacgccttcg aacggatgac gacggatgga ggtatcgcca cgatcgttca tgtatttgaa
     3481 ttcaatgttt tctttgccag aaccgtacaa caccacttta tgcacgttcg cgctcaggct
     3541 gccccacggc gcttcgacgt cgaacttata gtgatctgcc agcgatttca gcatctggaa
     3601 ataatagaag ttgcggcgat cccagccacg gatcgcacca cctgccagcg acagttccgg
     3661 attctggatc acgcggtcag gatcgaaata ttgctgtacg ccaaggccgt cgcaggtcgg
     3721 gcaggcacct gccgggttgt taaacgaaaa cagtcgcggc tccagttcac gcatactgta
     3781 gccgcaaatt gggcaggcga agttggcgga aaacagcagc tcttccgctt tcgggtcgtc
     3841 catatccgcc actaccgcgg taccaccgga aagctccagc gcggtttcaa acgactcggc
     3901 aagacgttgg gtaagatcgt cacgcacctt gaagcgatca accaccactt caatagtgtg
     3961 tttcttttgc agttccagtt tcggcggatc ggaaagatcg cagacttcgc catcaatacg
     4021 agcacggatg taaccctggc ttgccaggtt ctccagcgtt ttggtgtgtt cgcctttgcg
     4081 ctctttaatg attggcgcga gcagcatcag acgcttgcct tccggctgcg acagcacgtt
     4141 atccaccatc tggctgacgg tttgcgccgc cagcgggacg tcgtggtccg gacagcgcgg
     4201 ttcgccgacg cgggcgtaca acaaacgcaa atagtcgtgg atttcggtga ttgtccccac
     4261 cgtagaacgc gggttatgag acgtcgattt ctgctcaatt gagatggcag gagaaagccc
     4321 ctcaatatga tcgacgtccg gcttttccat cagtgacaga aactgccgcg cgtaggcgga
     4381 aagggattca acgtaacggc gctgcccttc ggcatataag gtgtcgaaag cgagcgagga
     4441 tttgccagaa cccgaaagcc cggtcacgac aatgagcttg tcgcggggga taacgaggtt
     4501 gatgtttttg agattatggg tgcgggcgcc ccgaacttcg atcttatcca ttcacctttc
     4561 ccggattaaa cgcttttttg cccggtggca tggtgctacc ggcgatcaca aacggttaat
     4621 tatgacacaa attgacctga atgaatatac agtattggaa tgcattaccc ggagtgttgt
     4681 gtaacaatgt ctggccaggt ttgtttcccg gaaccgaggt cacaacatag taaaagcgct
     4741 attggtaatg gtacaatcgc gcgtttacac ttattcagaa cgattttttt tcaggagaca
     4801 cgaacatggc cagcagaggc gtaaacaagg ttattctcgt tggtaatctg ggtcaggacc
     4861 cggaagtacg ctacatgcca aatggtggcg cagttgccaa cattacgctg gctacttccg
     4921 aatcctggcg tgataaagcg accggcgaga tgaaagagca gactgaatgg caccgcgttg
     4981 tgctgttcgg caaactggca gaagtggcca gcgaatatct gcgtaaaggt tctcaggttt
     5041 atatcgaagg tcagctgcgt acccgtaaat ggaccgatca atccggtcag gatcgctaca
     5101 ccacagaagt cgtggtgaac gttggcggca ccatgcagat gctgggtggt cgtcagggtg
     5161 gtggcgctcc tgcaggtggc aatatcggtg gtggtcagcc gcagggtggt tggggtcagc
     5221 ctcagcagcc gcagggtggc aatcagttca gcggcggcgc gcagtctcgc ccgcagcagt
     5281 ccgctccggc agcgccgtct aacgagccgc cgatggactt tgatgacgat attccgttct
     5341 gatttgtcat taaaacaata ggttatattg ttttaaggtg gatgattaaa gcatctgcca
     5401 gccataaaaa agaagcctcc gttatggagg cttctacgta tcaggtcaaa atcattggcc
     5461 actgtggcgg tgtctgattc atctcttcta tcatcaccga ctcgatgttg tgccagatgg
     5521 cggtgatgtc cgccattgtg atgccaagca accccattgc gaaccagcag gcggaaacca
     5581 cgcccagacc gctgctgatc actgcaagcc cgacataatc agaccgacga aaacggatgt
     5641 tgagcgtgct ggccagaaac atcattacgg cactaagaag ttgccagcga agaagaacca
     5701 cgccagtggt gagggtagcc atcaaacaat tcctctgaaa agagccgatg ccctggacag
     5761 cgcggcgttg ttcacgggag gcgtggtaca ctctggctat cgcggggctt gcagaacaca
     5821 aaaatgaaac actctgtttg tttcattaat tttgtgaact atatcacaat tgattgtttg
     5881 ttagccagat taggtcgtga cttttattgc tgtacagatt atgtggtttt tcagtggcat
     5941 taagggtata tcttcccgcc gcctctgtat tcctgtagaa aattaatttt gaatatcaat
     6001 gaattatttt catccaagtg acgattaaaa aggtatcaat ttcaaatcag gcaaaagtgc
     6061 tatttatacc gtaagattta tctaaagacg tcggtagcca gggtttcacc ttgcaatggc
     6121 cgggtataaa caggcaggaa attgatagca atgaatcatc gtgcacgaca ccaattactg
     6181 gcgttgccgg gcattatctt tttagttctc tttcccatca ttctttcgct atggattgcc
     6241 ttcctttggg caaaatcaga agtgaataat cagctccgaa cctttgctca actggcgttg
     6301 gataaatccg agctggtcat tcgccaggca gatttagtga gcgatgcagc tgaacgctat
     6361 caggggcaag tttgcactcc agcccatcaa aagcgaatgt tgaatattat tcgtggctat
     6421 ctttatatta atgaatt
//
LOCUS       DBFIFM010000228         6421 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.228, whole genome shotgun sequence.
ACCESSION   DBFIFM010000228 DBFIFM010000000
VERSION     DBFIFM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.228"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            1..355
                     /gene="ydjO"
                     /locus_tag="N7C74_004148"
                     /pseudo
     CDS             1..355
                     /gene="ydjO"
                     /locus_tag="N7C74_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(359..1750)
                     /gene="tcyP"
                     /locus_tag="N7C74_004149"
     CDS             complement(359..1750)
                     /gene="tcyP"
                     /locus_tag="N7C74_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HGU2433906.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHH"
     gene            complement(1883..2473)
                     /gene="ydjM"
                     /locus_tag="N7C74_004150"
     CDS             complement(1883..2473)
                     /gene="ydjM"
                     /locus_tag="N7C74_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU2433907.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2636..3304)
                     /gene="hxpB"
                     /locus_tag="N7C74_004151"
     CDS             complement(2636..3304)
                     /gene="hxpB"
                     /locus_tag="N7C74_004151"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HGU2433908.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3451..3987
                     /gene="yniB"
                     /locus_tag="N7C74_004152"
     CDS             3451..3987
                     /gene="yniB"
                     /locus_tag="N7C74_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HGU2433909.1"
                     /translation="MTYQQAGRIAVLKRIMGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGTRMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4028..4888)
                     /gene="yniA"
                     /locus_tag="N7C74_004153"
     CDS             complement(4028..4888)
                     /gene="yniA"
                     /locus_tag="N7C74_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HGU2433910.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4994..5284)
                     /gene="ghoS"
                     /locus_tag="N7C74_004154"
     CDS             complement(4994..5284)
                     /gene="ghoS"
                     /locus_tag="N7C74_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HGU2433911.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5385..6314)
                     /gene="pfkB"
                     /locus_tag="N7C74_004155"
     CDS             complement(5385..6314)
                     /gene="pfkB"
                     /locus_tag="N7C74_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HGU2433912.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1663 a         1591 c         1572 g         1595 t
ORIGIN      
        1 ctgtcgggag catttttgct gcgattcaaa aaaatgagag tctcctaaag tttccttcga
       61 taatatcaat gcttaatatc aatcttcttt ttaatccact aaatcttccg ggaatgggaa
      121 gtggtatact tgaagatatt atgagtattc cagatagttc acttagaaaa cgtctcgggt
      181 atgaggtttt aagcttctcg ctacaggcac attcattgag ccaggaatgt attgataagt
      241 tggacatttt ttcgctgacg atctgtttaa atatgagtca gtatgcatcg ccgcgatgga
      301 acatttaaaa agtaaggcaa cggccccttt acaaaacgga ccgttgccag cataagaatt
      361 aatggtgtgc cagttcggcg tcgtcttcgc tatccagaat ggctttatcg gtttgcttca
      421 gccactggct ggtcagcgtg ccagctgtca tcgagccact aacgtttagc gccgtacggc
      481 ccatgtcgat aagcggttca acggagatta acagcgccac cagggttact ggcaggccca
      541 tcgcaggcag cacaatcagt gcggcgaaag ttgcaccacc accgacaccg gcaacgcctg
      601 cggagctaac ggtaacaata ccgaccagcg tcgcaatcca catcgggtcc agcgggttaa
      661 tgccaaccgt aggcgcaacc atcaccgcca gcattgccgg atacaaaccg gcgcagccgt
      721 tctgaccaat ggtcgcaccg aaagaggcgg cgaaactggc gatggattca ggaacaccga
      781 gtcgacgcgt ttgtgcttcc acattcagtg ggatagacgc agcactggaa cggctggtaa
      841 aggcaaacgt cagcacaggc cataccttac ggaagtactt cagcggactc acgccattaa
      901 tgcccagcag aatgccatgc actgcaaaca taatcagcag accgaggtag gacgcgacaa
      961 cgaaacttcc cagtttgatg atgtcttgca ggttagaacc tgcaaccact ttggtcatta
     1021 gtgccagaac gccgtaaggg gtcaactgca tgaccaggcg aactagtttc atcacccagc
     1081 tttgcagggt atcgatagcg gttaagacgc gttcaccttt cggcgcatca tccttcagca
     1141 gtttcagcgc agctacaccg aggaatgcgg caaaaattac cacgctgata attgacgtcg
     1201 gattggctcc ggtcagatcg gcaaacgggt ttttcgggat aaaggacaag accagctgcg
     1261 gaacgctcag gtcagagact ttaccaacat agttactttc gatggcgttc agacgtgcag
     1321 tttctgcacc accctgaacc agaccttcag ccgtcaaacc aaacaggttg gttaccagca
     1381 caccgaccag cgccgcaatc agcgtggtaa acaaaagcgt accaatggtc agaaaactga
     1441 ttttgcctaa ctgagatgcg ttatgcagac gggcaaccgc gctcagaata gaggcgaaga
     1501 ctaacggcat aacgatcatt tgcagcagtt gaacatagcc gttaccaaca atgttaaacc
     1561 actgtacaga atctttaagt acctggctgt cagaaccata aatggtatgc agggcaaggc
     1621 caaaaactac acccatcacc agacccacca gcactttttt cgccagactc cactgtttat
     1681 ggcgggtctg agccagcgca aacagcagta caacgaacac cacgatgttc gcaattaatg
     1741 gaaagttcat ccccgttctc ctgatcttat tatttctatc actgcgtagc agtcatagtg
     1801 ttggcgcaag attaacagaa ctgtgagctg gctcttatat tcaaatggaa ttgcttatta
     1861 cagaaaggcg atttttgcct ttttagtatt caacctgatg cttaataagc cgatgaaact
     1921 ggatctgcaa ggtattgatc atttgcgatg accaacgaac agcgctgttt tcgggtaatg
     1981 aatgaggcat ccataccgac cagacaaata atgccatgca gataaaacgt tccagttggt
     2041 tacccttttg gggaaccagg ataggcaagc ggaaacgcca gcggcatggc cagagcaggg
     2101 gaacaccggc gggtgtcagc atatcggcaa gtatgtggct taaataaccc agcaccattc
     2161 cttgtagcgc atcagccgga atgaaccagc tttcaggaac cttaaggtaa aaggttgcca
     2221 gcagcgcaaa taccgccagc agactgtggg taaaaccacg atgcccaaaa gcgcgggcga
     2281 tcggttttga tatccacttt aatcgctgcc caagaaacga ctttgggtga tcgatgtccg
     2341 gtaacaaaca cgtcaggatt gcggaaggga caatatgcca ccagtcaccc tgtgccagca
     2401 cgggcgtcag ctcggcattt ttggcaaata ccgcacaagc aatagaaaag agaaggtgac
     2461 cttccgccgt catgataaaa ctcactaaac tgtcgatacg tacagtatag gatttttata
     2521 cagtgcacgg aagtggtgac ggtgtaactc tttctcttaa attgacgatt aatccgtcag
     2581 ttgaatgcaa attcagataa agaaccaggg gcagcgtgat cactgcccct ggtcattaac
     2641 cgagaaggtc ttttgcggtg agttctgtca gcgatgagag tttgacgttt gctaatacaa
     2701 aacgtggatc attttgcgct tctggcgcag gaacgacgat ggaacgcatg cgggctgctt
     2761 tagaggcgat catgccattt accgaatcct ccagcgctac gcaggtcagt gggtcaacgc
     2821 ccagttttgc tgcgcagtcg agatatactt gcggatgcgg cttgctgtaa ggcagttttt
     2881 cggccgaggc gagggcatcg aaactgtcgc gtaagtcaaa catggtcaac actttttcca
     2941 gcatatgtag tggtgacgcg gaggccagtc ccaccaataa accttgttct ttgcataacg
     3001 caacggcttc gcgcacgcct ggtaataatg gacgtgtctc ttcaaccagt gaaatggcac
     3061 gggcaataac ccgttctact acttcctgac ggcttggccc attccacggt tgccgggcgt
     3121 accaaagatc gaccaccata tcgatgcgta aacctaaggt gtccggcagc tcgttacgac
     3181 gggagatatc cacccccagg cttgccatca catccagttc ggctcgatcc cataaaggtt
     3241 ctgagtcgat aagtaatcca tccatatcaa aaattgcagc aagaatctga cgcggggttg
     3301 acattacaac ctctccttta gagcattatg tgtaaggtat taagccgtgc ttatgattaa
     3361 gcgcctatca tatcgccttt aaagaatggg cgaaaacgga tatctacagg taaactaagt
     3421 gaaacgacgc gtcttaatca aggagaactc atgacgtatc aacaagctgg acgcattgct
     3481 gttttgaaaa ggattatggg ttgggtgatt tttatacctg cattgatctc tacattgatt
     3541 tctttgctga aatttatgaa tacccggcag gaaaaccagg aaggcattaa tgcggtcatg
     3601 ctcgacttta ctcatgtcat gatcgatatg atgcaggcga atacgccttt tttgaatctg
     3661 ttctggtata actccccgac gcccaatttt aatggtggcg tgaacgttat gttctgggtg
     3721 attttcatcc tgatttttgt tggactggcg ttgcaggatt ccggtacccg gatgagtcgc
     3781 caggcgcgct tcttgcggga aggtgttgaa gatcaactca ttctggaaaa agccaaaggg
     3841 gaagaggggc taacgcgtga acaaattgag tcacgtattg ttgttccgca ccatactatt
     3901 ttcctgcagt ttttctcact gtatatcctg ccggttattt gtattgctgc aggttatgtg
     3961 ttcttttctc tgcttgggtt tatttaatca aaaggccgca cggagcggcc attatcttca
     4021 acccatatca cgcagctaat aatctatcca gtgactgctg agcaataacc aaatgctgac
     4081 cgccaaataa tcttgcacga tttaacagcg tgtagagttg gtaaaccggt tgacgttcaa
     4141 ggaaatcggc aggtagcggg gatactgact gatagccgtc atagatttgt ggcggctgtt
     4201 cagtatgcag cggtaacatc gccaggtcgc actctcggtc accccagtag caggccgggt
     4261 cgaaaatgta cgggccatcc ggacccagcg cacagttgcc ggaccataaa tcgccgtgca
     4321 acagagaagg ctgcggttga tgtgaggcga gacgctgctg aatatgctcg acgatggcat
     4381 cgatattgcc gaaagcgatc cctttttctg ctgccagttc caactgccag ccaatccgtt
     4441 gttcagcaaa aaacgttgac cagcgacgtt gccaggtgtt gggctgtgga gttgtggaga
     4501 gcgagttatc aaaatcgagt ccaaattgtg gttggtcgct ccattgatgt aaacgcgcaa
     4561 tttgctgacc aagaataaat gcgctatgcg catccagcgg acgaggtggg agataatcca
     4621 tcaccagaaa actgtagtca cggtcagcgc caactgccca aaccttaggc acggtgacgg
     4681 ttttactacg cgacagtaac tccagttggt cggcttcggc ggtaaaaccg ggaagcagtt
     4741 ccctttcatc acatttgacg aaaaagtcat gtcctgcata gcgcaaatgc catgcggcat
     4801 ggacttctcc gccaggcagt tcattacgca gttcgatttc gccttcacct aactgctcgc
     4861 tcaaaagacg actgattgcc tgccacatga tgtctctccc atgttgtctg ccagatcata
     4921 aggttagcgc aagaatgcgg tgtaaaaaca cgagctaacg cacaccacag actgattatt
     4981 gcccaaaaac catttatttt ctgttgctat tccattcctc ccaggtcgtg atggtgattt
     5041 caggatcttt gccggttgca ctttgggtaa gccccgaaac cagttctcta atttcttctt
     5101 cactttgggt actgataagt ccaaaagtgt tagtccccag ctcatggata tttccctcat
     5161 catccgtcat ggtgagcaaa aaaccgtcgc gagtcaggta attattcaac tcgttaattt
     5221 cagtcaatgt atcctcatgc aacatgacgg ttatgacata acggacaaga tcgccactgg
     5281 ccatagttca cccctttgtt atcacatgac ttttgagcat agtcggagaa acgcgttgcc
     5341 gacaggttgg tgatgattcc cccaatgctg ggggaatgtt tttgttagcg ggaaaggtaa
     5401 gcgtaaattt tttgcgtatc gtcatgggag cacagacgtg ttccctgatt gagtgtggct
     5461 gcactccccg cagctacgcc aaaacgaacc atctcttcaa gagaggcatt ttctgccagt
     5521 ttcagtgtca tcgcgccgac catgctatca ccagcgccaa cggtactctg gcttttcacc
     5581 ggtggtggca ccacctgaat gcagttttca ctatcaacac ccagcgctcc ttgtggaccc
     5641 agggaaacga caacccgttt ggccttgccg ctattaacga tttcctgcgc ggctttgcgg
     5701 acatcgtctg gctgggtgag ttcgcgattc accagcgcac tgagttcttt ttggttaggc
     5761 ttaaccaact cgatgttacc aatcgccagt gctgcactta acgcttcgcc agaactgtcg
     5821 atgatgcagc ggatcccttg gttttgcgca gcggaaatca gttgggttaa tttttccagc
     5881 ttcacacctg gcggcaggct tccgcttatg accaggatgg ccccggattc aatttccaga
     5941 acttgctctt caagctggcg aaactcatct tcatttaatg ccgcgcctgg cataacaaaa
     6001 cgatactgct caccgcttgc ttccacatgt acgtgtaaat tctgccgggt ccagtctttg
     6061 gcttctacag tagcgacggg gacattttca tccgccaaca gtgaaaccag gtgttcgccg
     6121 gtcgcgccac ccgccgggaa gatcgctgtg gcactgcctc caagatgggc aatggcgcgg
     6181 gcgacgttga tgccgccgcc cccgggttcg aacaccggtg cggtacagcg cagttttcct
     6241 tcgggataaa tttgcggggt aattgttgcg ctatcgagag agggcgcaag tgtcaacgta
     6301 tagatacgta ccatcatttc ctcctatagg ctgatttcag tctggcaccg aatcagcgga
     6361 aagtgaagaa attaacaata tgatttattg gagctttatt taaaattttg cagataaata
     6421 t
//
LOCUS       DBFIFM010000229         6373 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.229, whole genome shotgun sequence.
ACCESSION   DBFIFM010000229 DBFIFM010000000
VERSION     DBFIFM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.229"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(16..879)
                     /gene="potB"
                     /locus_tag="N7C74_004156"
     CDS             complement(16..879)
                     /gene="potB"
                     /locus_tag="N7C74_004156"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HGU2433913.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLARDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDN"
     gene            complement(863..1999)
                     /gene="potA"
                     /locus_tag="N7C74_004157"
     CDS             complement(863..1999)
                     /gene="potA"
                     /locus_tag="N7C74_004157"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HGU2433914.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            2249..3475
                     /gene="pepT"
                     /locus_tag="N7C74_004158"
     CDS             2249..3475
                     /gene="pepT"
                     /locus_tag="N7C74_004158"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HGU2433915.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(3524..4645)
                     /gene="roxA"
                     /locus_tag="N7C74_004159"
                     /gene_synonym="ycfD"
     CDS             complement(3524..4645)
                     /gene="roxA"
                     /locus_tag="N7C74_004159"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HGU2433916.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYTNGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            4894..6123
                     /locus_tag="N7C74_004160"
     CDS             4894..6123
                     /locus_tag="N7C74_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2433917.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFITQLVEQNRHTGLLLGEVKQEASVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
BASE COUNT         1682 a         1524 c         1641 g         1526 t
ORIGIN      
        1 tttgaatata aagcactaat tatctatttc tgttgccttc ttattcagca gacgagaagc
       61 gcgccagtaa accagcaaca tcaggcccat tacgatagtc agcgtaatgc tggtggctgc
      121 accaaacggc cagtcacgaa tattgaggaa ctgtaccttg atgacgttac cgatcagcag
      181 gtttttcgca ccgcccatca ggtcggacac atagaacagg cccatcgctg gcagcatcac
      241 cagcagacat ccggcaataa tccccggcat cgtcagtgga ataatgatgc ggataaaagt
      301 ctgtaacttg ctggcaccga gatcgcgcgc cgcctcaagc aacggcttat ccagtttttc
      361 gatactggag tacagcggca tcaccataaa cggtagcaga atgtaaacca gaccgataat
      421 caccgcactg ggcgtgaaca tgatacgaat tggtgtgtcg ataacgccca gccagagcaa
      481 aaactcgttg agatagcctt tggtgctgag gaaaattttc agcccgtaga tgcgaatcag
      541 tgagttggtc cagaagggaa caatcagcag aaacagcagc agcggacgca ccttgtgtgg
      601 cagcttcgcc agaaaccagg caaacggata gcccagcacc aggcaggcga gggtggcgat
      661 cagcgccata ttcagcgagt gcaatagcac ttcaaaatag agcggatcga gcagacgtgt
      721 gtagttatcc agcgtaaaga ccattttgac aaaactggcg tcgtcgcggg ccaaaaagct
      781 ggtgccaatg atcatcaggt tgggcagaaa gacaaacaac acaagccaac cgacaatagt
      841 gacaatcact acattctgga acttacttgt gttcttcatc agccagtacg acctcccagc
      901 tttctaccca attaatggcc attttttggt cgagagagtg gtcaaagtca ggatcgtctt
      961 cattgaagaa ttcgctgacc atcaccatct tgccattttc cagttcaaca accgactcca
     1021 gcgtcatgcc tttgtagtta cgctcgcgaa cgtaaccaat cagcccttca gcgtggttgt
     1081 cgtcgttaat ctcttcaaca cgtaagtctt ccgggcgcag cagaacatgc agtttttgcc
     1141 ccggttcaac ggcgaagtta acgtagatat tacattcgcg gccttcaacg ttggcgcgta
     1201 cgcgctgctc gtcgagacgt tcgatgacag tggcgttaaa catattgatt tcgccaatga
     1261 acccggcaac aaacaggttt ttcggctctt cgtagatttc acgcggcgtg ccgtcttgct
     1321 caatgcgacc atcgcgcatc accacaatcc tgtctgacat ggtgagtgct tcttcctgat
     1381 cgtgagtcac gaagacgaat gtaatgccaa gcttacgctg taacgctttc agctcgttct
     1441 gcatttgctt acgcagtttg taatccagcg ctgagagcga ctcatccagc aacaacagac
     1501 gaggcttgtt aaccaccgcg cgagcaatgg cgacgcgttg ctgttgacca ccagagagct
     1561 gatgcggttt acgttgagcg aaggtttcca gctgcaccat ccgcagggct tccatcacgc
     1621 ggggcgtaat ttcagcagcg ggggtttttt gcatgcgcaa cccaaaggcc acattttcga
     1681 acacggtcat gtgggggaaa agtgcgtagc tttggaaaac agtgttcaca tagcggtttt
     1741 ccgccggaac gtgggtgatg tcctcgttat ccaacatgat gcgtccggaa tcaacagttt
     1801 ccagacctgc aatcaggcga agaacggttg ttttaccgca gccagaaggg ccaagcagcg
     1861 tgaggaactc gccattgttg atagtcagat ccagctgggg aatgacctct ttaccatcaa
     1921 agcatttgcg aattcccgcc aattgcacca gcggtgaaag cgaactcggt tgtttattca
     1981 attttttact ctgtcccatg taaacgcaac ggatggctta ccgatgcggg gtttgtggtt
     2041 aaccaccttg gtgactctta atgagggcgg taattctacg gcaaaccgct tgaatcgcca
     2101 atctttgttg tgaattactg gcttagcttt atattcatta aggtaatgct gataaatatt
     2161 cccgcttgca ggggtaaaag tgacctgacg caatatttgt cttttcttgc ttcttaataa
     2221 tgttgtcaca aaaagtgagg gtgactacat ggataaacta cttgagcgat ttttgaacta
     2281 cgtgtctctg gatacccaat caaaagcagg ggtgagacag gttcccagca cggaaggcca
     2341 atggaagtta ttgcatctgc tgaaagagca gctcgaagag atggggctta tcaatgtgac
     2401 cttaagtgag aagggcactt tgatggcgac gttaccggct aacgtccctg gcgatatccc
     2461 ggcgattggc tttatttctc atgtggatac ctcaccggat tgcagcggca aaaatgtgaa
     2521 tccgcaaatt gttgaaaact atcgcggtgg cgatattgcg ctgggtatcg gcgatgaagt
     2581 tttatcaccg gttatgttcc cggtgctgca tcagctactg ggtcagacgc tgattaccac
     2641 cgatggtaaa accttgttag gtgccgatga caaagcaggt attgcagaaa tcatgaccgc
     2701 gctggcggta ttgcaacaga aaaacattcc gcatggtgat attcgcgtcg cctttacccc
     2761 ggatgaagaa gtgggcaaag gggcgaaaca ttttgatgtt gatgccttcg atgcccgctg
     2821 ggcttacact gttgatggtg gtggcgtagg cgaactggag tttgaaaact tcaacgccgc
     2881 atcggtcaat atcaaaattg tcggtaacaa tgttcatccg ggcacggcga aaggagtgat
     2941 ggtaaatgcg ctgtcgctgg cggcacgtat tcatgcggaa gttccggcgg atgaaagccc
     3001 ggaaatgaca gaaggctatg aaggtttcta tcacctggcg agcatgaaag gcaccgttga
     3061 acgggccgat atgcactaca tcatccgtga tttcgaccgt aaacagtttg aagcgcgtaa
     3121 acgtaaaatg atggagatcg ccaaaaaagt gggcaaaggg ttacatcctg attgctacat
     3181 tgaattggtg attgaagaca gttactacaa tatgcgcgag aaagtggttg agcatccgca
     3241 tattctcgat atcgcccagc aggcgatgcg tgactgcgat attgaaccgg aactgaaacc
     3301 gatccgcggc ggtaccgacg gcgcgcagtt gtcgtttatg ggattaccgt gcccgaacct
     3361 gttcactggc ggttacaact atcatggtaa gcatgagttt gtgactctgg aaggtatgga
     3421 aaaagcggtg caggtgatcg tccgtattgc cgagttaacg gcgcaacgga agtaagcgaa
     3481 aagggatgcg gcatgtgatg ccgcatccgg cttaaatcca aacttaccct tcgaagaacc
     3541 aatacccgct attgaccagc gccgcgagca tcgcgaggaa tgacggatct tccagcgcat
     3601 cgccaaaatt ctccgcagtc agcgcaatgt tgctggcgag tgcatccagt gccggacggt
     3661 gcggggaatc gatcttctca ccattggtat acacgtcgtc gccaatgcgc aatacgcgca
     3721 gaccacccag gcgcaccagc acatcacctt gtttcagcgc atcgtagatt tcatctggct
     3781 gataaggcgg ctccggcggc gcgatatcca gttcatgacg tgactgggat ataaactcgc
     3841 caaaccattg cttaaagtgt tccggctggt tgatcaattc gagcatcatc tcacgcagtt
     3901 tatccatctc ttgcggcaga acgtccgcag gatgagcgcg aggtggaaca tcaggatcgc
     3961 tgtagtagtt gccgcccagt tcgcgttgca gcacataatc ggcaaatccg ctaattaatt
     4021 cccgcgtatt tggcgcgcga aaacccacgg aatagttcat cgcattttcc agcgcgtagc
     4081 cttcatgcgg gaatcctggc ggaatataaa gaatatcgcc aggctccagc tcttcatcga
     4141 tgatggcttc gaacggatcg acctgtaaca gatccggatg tgggcagtgc tgtttcattt
     4201 gcagcttctc gcccactcgc cagcgacgac gtccggtacc ctgaatgata aacacgtcgt
     4261 actgatcgag atgcgggccg acgccgccgc caggtacaga aaaagagatc atcagatcat
     4321 caatacgcca gtccggtagt tcacggaacg gtcgcatcag cgcggcggtc ggctcatgcc
     4381 agtggttcac tgcttgcact aacaatgacc agttagtttc accgagatga tcgtagcttt
     4441 cgaacggacc gtggctgacc tgccattttc catcctggtg actgaccagt cgactgtcga
     4501 cttcgctttc catcgccaga cccgccaact cgtctggaga gatcgggtca ataaaattat
     4561 taaagccgcg ttttaacacc accgggcgtt tctgccagtg acgttcaaga aaatcgggcc
     4621 agttaagagt gagttggtat tccatgttaa gcatccacag gctggtatct gcaaccgatt
     4681 ataacggatg cttaacgtaa tgcgtgaagt atggacatat tttatcaatt agtgtttttt
     4741 gccgtttcat aatgtttcag attgttcatt atatttttga ttataaacag atttgtgatt
     4801 ttccttgttc cagcttgttt cacgttgtag ccctttgtgc aacgatcggt gtagttaatg
     4861 gtgtagttaa ttgtgatgac cggaatcaaa attatgagca gagcacttaa caaactgagc
     4921 gatacacagc tgaggaaaat caacggcaca cccgcccaaa aaacagcctt tcttaatgac
     4981 ggtggaaacc tgagcgtcag gcattcaacc agtggccttt taacctggta tttcacttac
     5041 agggccggaa cgggaagggg ggcaccaccg gaacgcatta agctgggaaa ttatcctgat
     5101 ctgagcctga aatcagccag ggaaaaagcc gcccagtgtc gcgcatggct ggcagagggg
     5161 aaaaatccac gtcatgagct taattacacc gtacaggaag cgttaaagcc ggtaacggtt
     5221 ggcgatgcgc tcacctactg gcttgagtcg tacgcaaagg aaaaccgcgt ggattatgcc
     5281 gccctgaaaa agcgccttaa taatcacgta atacagcaca ttggtgctat gccgctggat
     5341 aaatgcgagc tacggcactg gctggcctgt tttgaccagg tggcaaagcg aacgcctgtt
     5401 actgccggat tcttgctaca gacgtgcaaa caggcgctta agttctgccg gaggcggcgc
     5461 tatgcaatca gcaacgttct tgatgatatg agtgtggcgg acgttgggaa aaaaccggat
     5521 ataagcgagc gtgtcttaag caccaaagaa ctgggcgaat tattgcaggc actggacaaa
     5581 aaaatattct ccccctacta catcgcgtta atccgcctcc tgattgtgtt cggatgccgg
     5641 acggtagaac tgaggttatc ggagatcagc gagtgggatt ttaccgaaat gctctggacc
     5701 gttccgaagg agcacagcaa aacgaaggta gccatattcc ggcctatacc ggaagcaata
     5761 ctgccgttca tcacgcagct ggtggagcag aacaggcaca cgggcttatt gctgggggaa
     5821 gtgaaacagg aggccagcgt atcgcagtat ggaagattag cgcacaggag gcttaatcac
     5881 cctcactggt cactgcatga catccggcgc acctttacaa ccatgctgaa cgatttaggc
     5941 gttgatccgc atgtcgtgga gcagcttaca ggccaccaga tgccaggaat gcagcgagtt
     6001 tataatcatt cccgttatct ggatgcgaaa cgcaatgcgc tggatatgtg gacggagcgg
     6061 ttagggatac tggcgggaac acatgaaaac gtaaccacgc taccagtagc cagaagaaaa
     6121 taattttttt cgtgtttttt cagtatgcgc atactggata tgcgaacaga tacaacgtgc
     6181 aacaatgagc aacaatgtgg aacaactacg aacaagaggc gaaaaagcgt acggatttat
     6241 aagtagctga tttttaatgt gttactggtt ttttatacac tagcgaatca ctctttaaag
     6301 agcgagaaaa aaggcatgga acaatatttt atgcctttgt attaacatga atttaaaaga
     6361 ttttattctt act
//
LOCUS       DBFIFM010000230         6346 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.230, whole genome shotgun sequence.
ACCESSION   DBFIFM010000230 DBFIFM010000000
VERSION     DBFIFM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.230"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(133..276)
                     /gene="ypaB"
                     /locus_tag="N7C74_004161"
     CDS             complement(133..276)
                     /gene="ypaB"
                     /locus_tag="N7C74_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HGU2433918.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVINNCS
                     AIS"
     gene            404..2689
                     /gene="nrdA"
                     /locus_tag="N7C74_004162"
     CDS             404..2689
                     /gene="nrdA"
                     /locus_tag="N7C74_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGU2433919.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKHFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2916..4046
                     /gene="nrdB"
                     /locus_tag="N7C74_004163"
     CDS             2916..4046
                     /gene="nrdB"
                     /locus_tag="N7C74_004163"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGU2433920.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            4046..4300
                     /gene="yfaE"
                     /locus_tag="N7C74_004164"
     CDS             4046..4300
                     /gene="yfaE"
                     /locus_tag="N7C74_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HGU2433921.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4354..5004)
                     /gene="inaA"
                     /locus_tag="N7C74_004165"
     CDS             complement(4354..5004)
                     /gene="inaA"
                     /locus_tag="N7C74_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HGU2433922.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(5085..6275)
                     /locus_tag="N7C74_004166"
     CDS             complement(5085..6275)
                     /locus_tag="N7C74_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2433923.1"
                     /translation="MKEKLWTKDFWAITIISFIIFFVFYVLLTLLPIYISDRLHASPD
                     KAGLLVTLFLIAAIVIRPFAGQWVGKYSNKTILVLSSLAFLVVTALYPFCHSIESLLF
                     IRVLHGITFGVITTVKGTISARLIPASRRGEGISFFSLAMGLAMVVGPWIGLNMARWE
                     AFNMAFWLCTGVAAVGIILSLIMTVPPVISHADGSKPKMGFAAMFDRAALPFAMVTFF
                     MTFSYAGVSAFLALYARELNLMSAASNFLLCYAIFLMICRTFTGNVCDKKGPKYVVYP
                     CLLFFTVGLVVLGYTQGSVMMVVSGALIGIGYGSVTPVFQTQIISSVEPHKIGVANSL
                     FFNAMDAGLALGACVMGMMVAHTGYRMIYLLGALLVVVAGGVYALQMKGKSGVALVVA
                     KEIH"
BASE COUNT         1643 a         1717 c         1539 g         1447 t
ORIGIN      
        1 acattcgctt atatattgac cacaactgat acatcagatt atgtgatgac tcgtgcttag
       61 atcaattttt gcaatcatta gcaaaaagat taataaacca tctatatcaa tttatccaac
      121 ctattctgcc gttcaagaaa tcgccgaaca gttatttata acaaattttt ctcttcccat
      181 tgactttccc ggacaccttg tctgacctaa ggtgcgcgaa agccactttt tccttcctga
      241 gttatccaca aagttatgca cttgcaagag ggccattttc acactatctt gcagtgaatc
      301 ccaaacatac cccctatata tagtgttcta agcagcttcc cgtactacag gtagtctgca
      361 cgaaactatt gcggaaagaa ttccaaaaac aggtacgaca tacatgaatc agaatctgct
      421 ggtgacaaag cgcgacggta gcacagagcg catcaatctc gacaaaatcc atcgcgttct
      481 ggattgggcg gcagaaggac tgcataacgt ttcgatttcc caggtcgagc tgcgctccca
      541 cattcagttt tatgacggta tcaagacctc tgacatccac gaaaccatta tcaaggctgc
      601 cgcagacctg atctcccgtg atgcgccgga ttatcagtat ctcgccgcgc gcctggcgat
      661 cttccacctg cgtaaaaaag cctacggcca gtttgagccg cctgcgctgt acgaccacgt
      721 ggtgaaaatg gtcgagatgg gcaaatacga taatcatctg ctggaagact acacggaaga
      781 agagttcaag cagatggaca cgtttatcga tcacgaccgt gatatgacct tctcttatgc
      841 tgccgttaag caactggaag gcaaatatct ggtacagaac cgtgtgaccg gcgaaatcta
      901 cgaaagcgcc cagttccttt atattctggt tgctgcgtgc ctgttctcga actacccgcg
      961 tgaaacgcgc ctgcaatatg tgaagcattt ttacgacgcg gtttccacat ttaaaatttc
     1021 gctgccgacg ccaatcatgt ccggcgtgcg taccccgact cgtcagttca gctcctgcgt
     1081 actgatcgag tgcggcgaca gcctggattc catcaacgcc acctccagcg cgattgtgaa
     1141 atatgtttcc cagcgtgccg ggatcggcat caacgccggg cgtattcgtg cgctgggtag
     1201 cccgattcgc ggtggcgaag cgttccatac cggctgcatt ccgttctaca aacatttcca
     1261 gactgcggtg aaatcctgct ctcagggcgg cgtgcgcggc ggtgcggcaa cgctgttcta
     1321 cccgatgtgg catctggaag tggaaagcct gctggtgttg aaaaacaacc gtggtgtgga
     1381 aggcaaccgc gtgcgtcata tggactacgg ggtacaaatc aacaaactga tgtatacccg
     1441 cctgctgaaa ggtgaagata tcaccctgtt cagcccgtcc gacgtaccgg ggctgtacga
     1501 cgcattcttc gccgatcagg aagagtttga acgcctgtat accaaatatg agaaagacga
     1561 cagcatccgc aagcagcgtg tgaaagccgt tgagctgttc tcgctgatga tgcaggaacg
     1621 tgcgtctacc ggtcgtatct atattcagaa cgttgaccac tgcaataccc atagcccgtt
     1681 tgatccggcc atcgcgccag tgcgtcagtc taacctgtgc ctggagatag ccctgccgac
     1741 caaaccgctg aacgacgtca acgacgagaa cggtgaaatc gcgctgtgta cgctgtctgc
     1801 tttcaacctg ggcgcaatta atagcctgga tgaactggaa gagctggcaa ttctggcggt
     1861 tcgtgcactt gacgcgctgc tggattatca ggattacccg atcccggccg ccaaacgtgg
     1921 agcgatgggt cgtcgtacgc tgggtattgg tgtgatcaac ttcgcttact acctggcgaa
     1981 gcatggtaaa cgctactccg acggcagcgc caacaacctg acgcataaaa ccttcgaagc
     2041 gattcagtat tacctgctga aagcgtctaa tgagctggcg aaagagcaag gcgcgtgccc
     2101 gtggtttaac gaaaccactt acgcgaaagg gatcctgccg atcgatacct ataagaaaga
     2161 tctggatgcc atcgctaatg aaccgctgca ttacgactgg gaagctctgc gtgagtcaat
     2221 caaaacacac ggcctgcgta actccacgct ttctgctctg atgccgtccg agacttcttc
     2281 gcagatctct aacgccacta acggtattga accgccgcgc ggttacgtca gcatcaaagc
     2341 gtcgaaagac ggtattctgc gccaggtggt gccggactac gagcatctgc acgacgccta
     2401 tgagctgctg tgggaaatgc cgggtaacga tggctacctg caactggtgg gtatcatgca
     2461 gaaatttatc gatcagtcga tctctgctaa caccaactac gatccgtcac gcttcccgtc
     2521 aggaaaagtg ccgatgcagc agttgctgaa agacctgctc accgcctaca aattcggggt
     2581 caaaacgctg tattatcaga acacccgtga cggcgctgaa gacgcgcagg acgatctggt
     2641 gccgtcaatc caggacgatg gctgcgaaag cggcgcatgt aagatctgat attgagatgc
     2701 cggatgcggc gtaaacgcct tatccggcct acggctcggt ttgtaggcct gataagacgc
     2761 gccagcgtcg catcaggcac aggatgcggc gtgaacgcct aatccggcct acggctcgga
     2821 tttgtaggcc tgataagacg cgccagcgtc gcatcaggca caggatgcgg cgtaaaatgc
     2881 cttatccggc attaaactcc caacaggaca cactcatggc atataccacc ttttcacaga
     2941 cgaaaaatga tcagctcaaa gaaccgatgt tctttggtca gccggtcaac gtggcccgtt
     3001 acgatcagca aaaatatgac atcttcgaaa agctgatcga aaagcagctc tctttcttct
     3061 ggcgtccgga agaagttgac gtttctcgtg accgtatcga ttaccaggcg ctgcctgaac
     3121 acgaaaaaca catctttatc agcaacctga aatatcagac gctgctggac tccattcagg
     3181 gtcgtagccc gaacgtggca ctgctgccgc tgatctcgat cccggaactg gaaacctggg
     3241 tagaaacctg ggcgttctca gaaacgattc attcccgttc ctatactcat atcattcgta
     3301 atatcgttaa cgatccgtct gttgtgtttg acgatatcgt caccaacgag cagatccaga
     3361 aacgtgcgga agggatctcc agctactacg atgagctgat cgaaatgacc agctactggc
     3421 acctgctggg cgaaggcacc cacactgtta acggtaaaac cgtgaccgtt agcctgcgcg
     3481 agctgaagaa aaaactgtat ctctgcctga tgagcgttaa cgcgctggaa gcgatccgct
     3541 tctacgtcag ctttgcttgt tcctttgcct ttgcagaacg cgaactgatg gaaggtaacg
     3601 ccaaaattat tcgcctgatt gcccgcgacg aagccctgca cctgaccggt actcagcata
     3661 tgctgaatct gctgcgcagc ggtgcggatg atccggagat ggcggaaatt gccgaagaat
     3721 gtaagcagga gtgctatgac ctgtttgtcc aggcggcaca gcaagagaaa gactgggcgg
     3781 attatctgtt ccgtgacggt tcgatgattg gtctgaataa agacattctc tgccagtacg
     3841 ttgaatacat caccaatatc cgcatgcagg cagtcggttt ggatctgccg ttccagacgc
     3901 gctccaaccc gatcccgtgg atcaacacct ggctggtgtc tgataacgtg caggttgctc
     3961 cgcaggaagt ggaagtcagt tcttatctgg tcgggcagat tgactcggaa gtggacaccg
     4021 acgatttgag taacttccag ctctgatggc tcgcgttacc ctgcgcatca ctggcacaca
     4081 actgctgtgc caggatgaac acccttccct tctggcggcg ctggaatccc acaatgtggc
     4141 ggttgagtac cagtgccgcg aaggttactg cggctcctgt cgcacacgcc tggttgcagg
     4201 ccaggtggac tggattgccg aaccgttagc ctttattcag ccgggggaaa ttttgccctg
     4261 ttgttgccga gcaaaaggcg atattgaaat cgagttgtga tcgggtacta gcgccagata
     4321 cgacgcttac gcgtattatc tggcctacaa cgattacatg gcgtagtaat acgcttttac
     4381 ctgctcccag tccgctttgg ggatcggctc cagatatttt tccagctggc ggaagtcatg
     4441 attaatcgct ttatcgcgac gcaagcggcg acgacttttc tccagatcaa gaaaaccagc
     4501 ttctgcctta ccttctgttt tcacatagat atggcgaaca taacaacagc catgctgacg
     4561 attaacgcta tgcattttct taaacgccag cgccactgct ttcaacatgg cttgccgtac
     4621 ttcgtcagaa tagggcgtta ctgcatgctg ggcataccag tcagcaatgc tgatgaaccc
     4681 cgccatatct tcagtcacca gcaacgcgcg ccattccccc tcaattttca ccgcttcacc
     4741 aaaaacgatc ttcggcacga tgacaccggc ccgttccagt tctttaatta ccgcaacctc
     4801 acggacaatc gttggtcggc cgaaaggata acgtacggaa tgaaacagat gatgcgtcat
     4861 gcgctttaca tatagctttt tgccgttgcg ctcgacgcat tgcaccccgc tcataccatt
     4921 acggcgatag ttaggctctt caacccagtc gccctctgtt gcccaccagt gattaaattc
     4981 gtcgtgcttt gctgaaactg ccatacccta tcgcctgtca ttttttatta acgacaatga
     5041 ctataggtgg ttacctgcga aaaatcttaa tgaaacgtgt cgtattaatg aatttctttt
     5101 gccactacta gcgcgacacc gctttttccc ttcatttgca gcgcatagac tccaccagcc
     5161 actaccacta atagtgcgcc cagcagataa atcattcggt agccagtatg tgcaaccatc
     5221 atccccatca cacaggctcc cagcgccagg cctgcatcca tcgcattgaa gaagagggag
     5281 tttgcgacac cgattttatg cggttccact gaactgataa tctgcgtctg aaaaactggc
     5341 gtcacggaac catacccgat accaatcaac gcgccagaaa cgaccatcat tacgctgccc
     5401 tgggtgtagc cgagaaccac cagcccaacc gtaaagaaca gcaggcaggg gtaaaccaca
     5461 tatttcgggc cttttttgtc gcaaacattg ccggtgaagg tacggcagat catcaggaag
     5521 atggcgtagc agagcaggaa attactggcc gccgacatca gattaagttc gcgggcgtaa
     5581 agcgccagaa aggcagaaac cccggcatac gaaaaggtca taaagaatgt aaccatggca
     5641 aacggcaatg ccgcgcgatc gaacatggcg gcgaagccca tctttggctt tgaaccgtcg
     5701 gcatggctga taaccggcgg cacggtcata atcagcgaca ggataatccc caccgccgca
     5761 acgccagtac ataaccagaa agccatatta aaggcctccc agcgcgccat attcaggcca
     5821 atccacggcc cgaccaccat tgccagcccc attgccagag agaaaaaact gatgccctcc
     5881 ccacgtcggg aggccgggat cagccgcgcg gaaatcgttc cctttaccgt tgtgataacc
     5941 ccgaaggtaa taccatgaag caccctaata aaaagcagtg attctattga gtggcaaaaa
     6001 ggatacagcg cagtgaccac caaaaaggcc agagaagaga gcaccagaat agttttattc
     6061 gaatatttac ccacccattg cccggcaaag ggtcgaataa caatcgccgc aattaaaaat
     6121 aaagtcacca acaaacctgc tttatcagga gaggcatgca agcggtcaga gatataaatt
     6181 ggcaacaatg ttagtaaaac ataaaagacg aagaaaataa taaagctgat gatggttatt
     6241 gcccaaaaat ccttcgtcca taacttctct ttcatactct accgcctgtc aggatgacgc
     6301 acgcagacaa cactcccgtt actgcggttg cgtcaaattg tgatgt
//
LOCUS       DBFIFM010000231         6331 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.231, whole genome shotgun sequence.
ACCESSION   DBFIFM010000231 DBFIFM010000000
VERSION     DBFIFM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.231"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(23..1399)
                     /locus_tag="N7C74_004167"
     CDS             complement(23..1399)
                     /locus_tag="N7C74_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGU2433924.1"
                     /translation="MRQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDASSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIEELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGSLGTVYQRFCNGHFVACDQDEARQICTASNAPAARGR
                     RYAQGADV"
     gene            complement(1396..2217)
                     /locus_tag="N7C74_004168"
     CDS             complement(1396..2217)
                     /locus_tag="N7C74_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGU2433925.1"
                     /translation="MGVVKLADYRPQLEVVEHRVADTEDGFMRVANEITDSLLMADLT
                     VRQLKVMLAIMRKTYGFNKPMDRLTNTQIAAMTGIHHTHVCAAKRQLIERKFLIADGV
                     KIGVNKVVSQWISQDSLTLAKTANKTLAKSANGYKPSQLNTKDNIQKTINTNTPLPPN
                     GDGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQLKTP
                     NVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA"
     gene            complement(2204..2365)
                     /locus_tag="N7C74_004169"
     CDS             complement(2204..2365)
                     /locus_tag="N7C74_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433926.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            complement(2398..2694)
                     /locus_tag="N7C74_004170"
     CDS             complement(2398..2694)
                     /locus_tag="N7C74_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HGU2433927.1"
                     /translation="MERTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITKKKSPAATEDFKQIDMQF"
     gene            complement(2836..3051)
                     /locus_tag="N7C74_004171"
     CDS             complement(2836..3051)
                     /locus_tag="N7C74_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2433928.1"
                     /translation="MSNLRKYRESLNISQTTLAKAVGCTQGAIGHWESGRRFPDLKTC
                     RALVACLNKLGAKVSLDDVFPPEHKAA"
     gene            3127..3822
                     /locus_tag="N7C74_004172"
     CDS             3127..3822
                     /locus_tag="N7C74_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HGU2433929.1"
                     /translation="MDGSSTERNNKDMKMKWYELARSRMKELGITQEKLAEELGMTQG
                     GIGHWLRGSRHPSLSDIGVVFKYLGIDNISFNHDGTFSPVGEYSSAPVKKQYEYPVFS
                     HVQAGMFSPELRTFTKGDAERLVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPDGML
                     ILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSVVG
                     KVIASQWPEETFG"
     gene            4324..4845
                     /locus_tag="N7C74_004173"
     CDS             4324..4845
                     /locus_tag="N7C74_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433930.1"
                     /translation="MAKSNVSVQAFKDFLEELMSLNIMKEATARNLKNSSARLLTVVQ
                     EEEMGDVTQLDVNELAERYINATEPKPSDSSITAYKSRMESAIKKFVAFQSGEEIPYT
                     PIDKESSEEKDLTGEPTKVEGKANALHTYDLPVVLRPESGVTVTIKGIPNDITNEEAE
                     RISSILKVYVRPQ"
     gene            5414..5596
                     /locus_tag="N7C74_004174"
     CDS             5414..5596
                     /locus_tag="N7C74_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433931.1"
                     /translation="MEQTGRLFKQRRLSTTWLKSQITQPHKLWDAMPKQPSQEELRDC
                     IAKVYSGGIYVQKNRI"
     gene            5574..5846
                     /locus_tag="N7C74_004175"
     CDS             5574..5846
                     /locus_tag="N7C74_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HGU2433932.1"
                     /translation="MSRKTEFKGTAASRRRARRANLQSQEAISSDKLHRPTPSRVVLQ
                     CKRKPAMRAEVITLTTLTRKYEGSTCLPNVALYAAGYRKSKQLTAR"
     gene            5905..6156
                     /locus_tag="N7C74_004176"
     CDS             5905..6156
                     /locus_tag="N7C74_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433933.1"
                     /translation="MEFHESAICDFRANANSVKPQPIAVLFKTMGAWAVLCFAADDTD
                     ARMAIGQEMEMDPTNDEFIIYGAPSNYLLDTCNIYNKAA"
BASE COUNT         1701 a         1510 c         1435 g         1685 t
ORIGIN      
        1 attaactcag tgatgtagat ggtcatacgt cagccccttg tgcatatcgt ctgccacgcg
       61 cagcaggtgc atttgatgct gtgcaaatct gtctggcttc atcctggtca catgcaacaa
      121 agtgtccgtt acagaaccgc tggtaaactg taccaagcga gccaaaacgg tttttcgtca
      181 cgatgatttc agcaaatggc gcggcgctac tgttctcatc atataccgct tcccgataga
      241 gcatgatgat tgagtctgcg tcctgttcaa tgcttcctga atcacgcaaa tctgcgtttg
      301 tcgggcgttt gtttggtcgc ttctcaacat cgcgcgaaag ctgactcagg gagataaccg
      361 gtgttttcag gtctttcgcc atcgccttca ggcttccgga gatgtgagca attgcgaggt
      421 cgttgcggtc tgctttcggc ttctcaatca ggccaagata atccgccatg atgagtgaca
      481 ggtttggatt ttcctgctta tgccgttctg cgattgagcg tatttcttcg accgataacc
      541 gcgaagcatc gactacccat acatccaaat ctgcaagctg actcatgccg ttagcaacac
      601 gtgcccagcc ctcgtcatcc atcgatgcag gatttcgcag tacgctaacc gacatcctcc
      661 cggcgttggc aatgcttcgc tctgcaatct gcaatgcgct catttccatt gagaaaatca
      721 ataccccgcg ccggacgtca gaaccaggaa taacgcggct tgccacacct tcggcaatct
      781 tcagcgccag ttcggttttc cccataccag gacgagcggc gataatcacc aggtcttctg
      841 cgttcatccc tccagtgata gcgtcaagct cttcgattcc ggtcttcagg gtatctgact
      901 cttctccgtt cctcagacgc ctgtcaagcg tgtcagtgta gtcagtgatg atttccccta
      961 accgtacagg tttaacctcg tcacggggct ttctgatggc tgaaagacgc tttacaagtt
     1021 catccatcgc ctgactcgat gcgtcgatgg ttccgctttg gattggttca cgcatttcat
     1081 ccatgatttc cagcaccaga cggcggtgat agttatccgc gaccattccg gcatatccct
     1141 tcaggtttgc ggcactcggg cagtttttgc tggtcatcag gattgacgtg aaatgctcct
     1201 ctccgcacgc ctcggcaacc atcagcgcat cgattaggtt tctgtttctc gcctgcttgc
     1261 gaataacctc gaaggctttc cggtagagcg gaattgaaaa cgcttccggc tccagcgttg
     1321 ccagaacgtc actggcggtt ggagttaatc caccaatcag caggccaccg ataacgctag
     1381 cttcgatatc ctgtctcatg caatccccct gtctgcaaac ttcccttccc gaactcccgt
     1441 taacgaatct tccctcagca ggtaatcaaa atctgccgtc cagcccgtgt cgttgtctcc
     1501 gaagtaaaac ggcttggcct gatgcacaaa cgccctgaca tacgctctga aaccgtccac
     1561 gtttggcgtt ttcagttgcg ggataatttt cttcaggcgg cgttttcgtt tctcgttgac
     1621 cgcaacagcg tgtggaagtc tgtcaccgac ttcggtgttg taggcgttca ggaaggattc
     1681 gtagtcgatt cgttctgcct tgcgacgttc aggtttaacc tgcccatcgc catccccgtt
     1741 agggggtaag ggggtatttg tatttattgt cttttgtata ttgtcttttg tgtttagctg
     1801 acttggctta tacccattag ccgacttggc taatgtttta ttagctgttt tagctaatgt
     1861 taagctgtcc tggctaatcc actgagaaac caccttgttc actccgattt tcacgccatc
     1921 agcaatgagg aatttacgct caataagctg gcgcttggca gcgcaaacat gagtgtgatg
     1981 aatacctgtc atggctgcta tctgcgtgtt tgtgagtcga tccatcggct tattgaatcc
     2041 gtatgtcttg cgcatgatag cgagcatcac cttcaactgc cggacggtta aatcagccat
     2101 cagcagactg tcggtaatct cgttagcaac gcgcatgaaa ccatcttcgg tatctgccac
     2161 gcgatgctcc acgacctcca gttgaggcct gtaatcagct aacttaacga cgcccatgtt
     2221 tcactcctgc tttggctagt ctgtaaacac caacaaggcg ctctgcgaac gccctgttat
     2281 ttgctgcggc taccactaat ccctcaggtg aatcagggtg tcgaatctct tctttttcct
     2341 ggtatttctt acgacgtttt gtcataatta ctcctgtgga ttgatccagt aattccctca
     2401 gaattgcata tcaatttgct taaaatcctc ggtggcagcc ggggattttt tctttgtgat
     2461 ttcatcaagc gcatacttaa aagccctgct aatcggactg atgtctgatg ccattccgaa
     2521 agcacacaag accgaagcaa taaaccgcca gtccgttctg cttatcttcg attcatgaca
     2581 gccaatcatc tttgccagac cgcgctgtgt aagcgttgac aggttgatga gtaaatctgt
     2641 ttctgcgcga tcaacgtcgc gctgtgatag tttgctgtaa cttgttcgtt ccatttctta
     2701 agatttccaa tagtgaatag ttagttgaaa ggtatgcgtg gaaacgcata tggccttagt
     2761 tggtcagata tcttgggact cgcttttcag cgacgtagga cgaatgtccg ttgttacaaa
     2821 gagcggctcc gcttattaag cggctttgtg ctccggcggg aacacgtcat caagacttac
     2881 ttttgcgcct aacttgttta gacacgcaac aagagcacgg catgttttaa ggtctgggaa
     2941 gcgacgacca gattcccaat gtccgatagc tccctgtgtg catccaactg ccttagcaag
     3001 tgttgtttga gagatattca gtgactctcg atattttcgt aggttgctca tatgccctcc
     3061 atagtaacca cgaataaaaa aatacaatat gtacttctca aatacaagta aaaatacaca
     3121 ttgtgcatgg atggttccag tacagagcgt aataataagg acatgaaaat gaaatggtat
     3181 gaactggcta gatccagaat gaaagagctc ggcataactc aagagaagtt agccgaagag
     3241 ctaggtatga cgcagggtgg aattggacac tggttgcgcg gatctcgtca tccatctctt
     3301 agtgatattg gtgtggtgtt taaatacctt ggtattgata acatatcatt caaccacgac
     3361 gggacatttt cacctgttgg cgaatactca tcggccccag ttaaaaaaca atatgagtac
     3421 cctgtttttt ctcatgttca ggctgggatg ttctctccag aactcagaac ctttaccaaa
     3481 ggtgatgcgg agagattggt aagcacaacc aaaaaagcca gtgactctgc attctggctt
     3541 gaggttgaag gtaactcaat gaccgcacca acaggttcca aacctagttt tcctgacggg
     3601 atgttaattc tggttgaccc tgagcaagct gttgagcccg gcgatttctg catagccaga
     3661 cttggtggtg atgaatttac cttcaagaaa ctgatcaggg atagcggtca ggtgtttcta
     3721 caaccactaa acccgcaata tccaatgatc ccatgcaatg agagttgttc cgttgtgggg
     3781 aaagttatcg ccagccaatg gcctgaagag acgtttgggt aaagaggata gatggagttt
     3841 aatggccatg agtatcaagc ggtcaaaaag aagttcatct attcattgaa agcataaggc
     3901 catctgagta tatccgtaat gatctggata ttgtctataa catcaatgac cggacgatag
     3961 atatcggtga gcagcgtcct gtatggcagg gtgaggcagg tgaaaaaacg tcctgccatc
     4021 aacaagaatc aagtgcatcc gctctctgga cagatggaaa atttattgga tgcaaaaaga
     4081 tatgaaataa cacctgtata gcacggagct ttctctgacc gatgcacttg aagttgttag
     4141 ggctgaccca gcctgctgtt tctttaggta agagaaaaga gatttaggag atgaaaggtc
     4201 gcagaggtgc ggcctttttt attgagagtg gatcttgaac ggaaattctc aagacttgag
     4261 tcttgcatgc aaatcaattc ctggataaac tcgatctgag tcaataactt agagagagag
     4321 caaatggcaa agtcaaacgt tagcgtgcag gcattcaagg acttccttga agagcttatg
     4381 tcgctgaaca taatgaagga ggccaccgct cgaaatttaa aaaactcatc cgctcgcctc
     4441 ttaacggtag tccaagaaga ggaaatgggt gatgttactc agcttgatgt gaatgagctt
     4501 gccgagcgat acatcaacgc aactgagccg aagcctagcg acagcagcat tactgcatat
     4561 aaaagccgca tggaaagtgc aatcaaaaag tttgtagctt tccagtctgg tgaagaaatc
     4621 ccatacactc cgattgacaa agaatccagt gaggaaaaag atttgactgg cgaaccaaca
     4681 aaagtcgaag gcaaggctaa tgcacttcat acctatgatc ttccagtagt tcttcgacct
     4741 gaatcagggg ttacagtaac gattaaaggc attcctaacg atatcacaaa cgaagaagcc
     4801 gaacgcatct cttcaattct gaaggtttac gttcggcctc aataatgcaa aagcattcag
     4861 cacaatgtcc aactccccag tccgacattg atgctgttta accagagcct ccaaaaggag
     4921 cccttgttaa ggtacacaat atttgcgatg taaccttagc gcgtctggta cattttttca
     4981 agcggttgta gggctgccgc caatatgaga aaacacagat gtctacatac aatttgaatg
     5041 accaattcga tcgagaagtt catgtgaatg cctatgaacg gataaggcat ggaaatcttg
     5101 aacatgtgtg tgagcattat cgctcaaggc cacaccgcta atcatcaacc cggcctccat
     5161 gccgggtttt cttttcctct cgcccaaaaa aacacataac caattgtatt tattggaaaa
     5221 taaatagata caactcacta aacaacgcaa ttctgatctc tccttacatc gccgaggcaa
     5281 tacacccacg ctaaaaaaca atactattaa atacaaagcg ttgtaaaaaa acgccccgtt
     5341 ttagaacaaa ttgtattgac caagtgaagt acatatcgta ctatttaacc atcagcagga
     5401 cgctggaagc caaatggaac agactggcag gctctttaaa caacgtcgac tctcgactac
     5461 gtggctgaaa agccagatca cccaaccaca taagctgtgg gatgcaatgc cgaagcaacc
     5521 gtctcaggag gagcttcgag attgcatcgc caaagtttat tcgggaggaa tctatgtcca
     5581 gaaaaacaga atttaaaggc accgcagctt ctcgccgtag agctcgtcgc gcaaacctgc
     5641 aaagtcagga ggcgattagc tccgacaaac tacacaggcc aaccccatca cgagtggtct
     5701 tacaatgcaa acgcaaacca gcaatgagag cagaagtaat aacactgaca acgttgacca
     5761 gaaaatatga aggctcaact tgtcttccaa atgtagctct ttacgcggca ggctaccgta
     5821 aatccaaaca actgacagca agatgacttg tgttggtcgc cagaaaatga aattaggcag
     5881 caaaccactt atttgaggtg atatatggaa tttcatgaaa gtgcgatttg tgattttcgc
     5941 gctaacgcaa attcagtaaa accacagcca attgcagttc tttttaaaac aatgggtgcg
     6001 tgggctgttt tatgcttcgc cgctgacgac actgacgcaa gaatggcaat aggacaagag
     6061 atggagatgg acccgacaaa cgatgaattc ataatttatg gcgctccatc taattactta
     6121 cttgatacct gcaacattta caacaaggct gcctgatggt ggcctttatt tttggcataa
     6181 acaacagaat aaacacagca ctgtgtattc attccaacga gtgaatacac ggagcaatgt
     6241 cgctcgtaac taaacaggag ccgacttgtt ctgattattg gaaatcttct ttgccctcca
     6301 atgtgagggc gattttttat ctgtgaggat a
//
LOCUS       DBFIFM010000232         6331 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.232, whole genome shotgun sequence.
ACCESSION   DBFIFM010000232 DBFIFM010000000
VERSION     DBFIFM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.232"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(108..2141)
                     /gene="betT"
                     /locus_tag="N7C74_004177"
     CDS             complement(108..2141)
                     /gene="betT"
                     /locus_tag="N7C74_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HGU2433934.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2270..2857
                     /gene="betI"
                     /locus_tag="N7C74_004178"
     CDS             2270..2857
                     /gene="betI"
                     /locus_tag="N7C74_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HGU2433935.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRHQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2871..4343
                     /gene="betB"
                     /locus_tag="N7C74_004179"
     CDS             2871..4343
                     /gene="betB"
                     /locus_tag="N7C74_004179"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HGU2433936.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCNAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGLDNGAWVAPTVFTDCSDEMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4357..6045
                     /gene="betA"
                     /locus_tag="N7C74_004180"
     CDS             4357..6045
                     /gene="betA"
                     /locus_tag="N7C74_004180"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HGU2433937.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAM
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDCKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKMADMIRGKEALPRSTAGYFVANGMPVRAKKMSRDLN"
     gene            complement(6047..>6331)
                     /locus_tag="N7C74_004181"
     CDS             complement(6047..>6331)
                     /locus_tag="N7C74_004181"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433938.1"
                     /translation="FLSSLQEPFSYMLTVSGSEQVTIARNTSGVILKLSGISPAVYQC
                     IHSTFISEIERVSLEFAGEVVTMKHESYPYVLYNDSVPIPGTVTLNLIGS"
BASE COUNT         1534 a         1769 c         1751 g         1277 t
ORIGIN      
        1 ttcaggatat gacatgacaa tcatatataa tatatgcata attttttaca gcgagtcaat
       61 gattcccgcg ccatatgcag acatggcgcg gttttatgca ataacaatca cgcgtccggg
      121 aacatcacgc tatggcccgg cgcttcacga tggagatgaa taaagttaag gtgccgctcg
      181 tactggtcaa gaatatcggt gatcacctgc tctttgctgt agtccatcag gtcgttgccc
      241 tggctgcctt ctaacaggaa ggtttccagc cggtagtagg tcgatttccc gctacgtgcg
      301 cggtaggtaa agcccggcac cgagtattgc tgcggccaaa tctgatagac aaagttttgc
      361 tcttcgccca tatgcaccaa caaatccaga tgccccaact gttgtccctc ttccggcggc
      421 aggcttttta gctccacgta cgcgccgcgc aaccgcaact cctgcgccac ttcttccatt
      481 gccgggtaac agaccgtctc catcatctgt ttagtgtaac gcgtgcccgg ataattcatc
      541 aggcgcgaga gacgtttttt ccagctcagg cgatcctgaa gccccagcgg tcgcggtgcg
      601 gtatcgcggt tggcactttc acggcggtaa tcttctacct tcagagattt atacaacccc
      661 gccatcacga agaagatcac aaagctgaac ggcagcccca taatcaccgt ggtgttttgc
      721 agcgcggata tcccgttggt catcagcatg ccgagcgtca gcaggccaat cgccaccgac
      781 cagaagacgc gcagccagcc gggggcgtcg ctgttgatat ctttaagctg cgaggtgaaa
      841 ttccccagca ccagcgcccc ggagtccgcc gaggtcacat aaaacagcag gccagtaatg
      901 gtggcgacgg aggcgctaaa ggtaaacgcc ggatactgcg ccagcaggct gtagaagccg
      961 cgctccggat ggaccatcgc ttcctcggca aatgccgcgc cgccgtggat gatttcatac
     1021 agcgcgctat tgccgaacac cgagagccat aacagcgtga aggtaaacgg aataatcaac
     1081 gtgcccagca cgaactggcg aatggtacgc ccacgcgaga tacgcgccag gaacaagccg
     1141 acaaacggcg accatgccac ccaccatgcc cagaagaaga gcgtccagtt attcatccac
     1201 tcaaccgggc ggtcgaaggc aaaactgttg agcgtcatgc ccataaagcg attcacatag
     1261 tcgccaacat tcagcaccag tgcattaagc aggaacgaag tgtcgcccat aaacaacacg
     1321 aacaggatca atcccagcgc cagcgcgaca ttaagctccg ataacacgcg aatgccctta
     1381 tcgacaccgg atgtcaccga gatagtggcg attatcaccg acaaggcgat cagtgccgct
     1441 tttgccgcca tcgaatcggg aatatcaaac agtacgctca agccatagtt aagctgcacc
     1501 acaccgatac cgagcgtagt ggcaataccg aagatagtgc cgatcaccgc tgcaatatcc
     1561 actgagtgac ctatcggccc gttaatccgt ttaccgaaga tcgggtacag cgccgagcgg
     1621 atggtgagcg gcaaattata acgatagcta aagtatccga gcgccatgcc catcagcgca
     1681 tacatcgacc agccggttaa gccgtagtga aacagcgtcc agaccatcgc ctgacgcgcg
     1741 gcctcaatcg tctgtcccgc gccttccggc ggctgcatat actgcgttac cggttcggct
     1801 acggaaaaga acatcagatc gataccgatc ccggcagcaa acagcatcgc cgcccaactc
     1861 agcaggctga attccggttt ggattgttct ggcccgagct tcaccgaacc aaaacgcgaa
     1921 caagcgatac agaccacaaa gacaatatag agcgttgccg ccagcagata gtaccaaccg
     1981 aaggttttag aaacccagtc cagcgtgcgg ccaatccaca gggccgagaa gtcgcgaaac
     2041 aggatcgttg tcagggaaaa caacaaaatc agtccggcgg aggtgtaaaa caccaccgga
     2101 ttgattttgt ccttttccct gctgtgtgaa aggtctgtca tccagtatcc ccactgttat
     2161 tgttaccatt taaaatcaaa ttcgtaacaa ttaagacaca ttttatattg aacgtccaat
     2221 caataaccgc tttaatagat aaacaacgct gatgaatgga gtggcaaaaa tgcccaaatt
     2281 ggggatgcag tcgatccggc gcagacaact gatcgacgcc acactggaag caataaatga
     2341 agtgggcatg cacgatgcaa cgatcgcgca gatcgcccgc cgtgcaggcg tttctacggg
     2401 gatcatcagc cactatttca gggacaaaaa tggtctgctg gaagcaacca tgcgcgatat
     2461 caccagtcag ctgcgtgacg cggttttgaa tcgattacat gcacttccgc agggcagtgc
     2521 agagcagcga ttacaggcga ttgttggcgg aaacttcgat gaaacgcagg tgagcagtgc
     2581 ggcgatgaaa gcctggctgg cgttctgggc cagcagtatg catcagccga tgctctatcg
     2641 tttacagcag gtcagcagcc gccgcttgct gtcgaatctg gtgagcgagt ttcgccgcga
     2701 attgccgcgc caccaggcac aggaagcggg ctacggcctt gccgcgctga ttgacggatt
     2761 atggctgcgc gcggcactga gcggcaaacc gctggataaa cctctcgcac actcgctgac
     2821 ccgtcacttt attactcagc atttacccac cgattaaccg aggagacgtg atgtcccgaa
     2881 tggcagaaca gcagctttat atacatggtg gttatacctc cgccaccagc ggtcgcacct
     2941 tcgagaccat taacccggcc aacggtaacg tgctggcgac cgtgcaggcc gccgggcgcg
     3001 aggatgtcga tcgcgccgtg aagagtgccc aacaggggca aaaaatctgg gcggcgatga
     3061 ccgctatgga acgctcgcgt attctgcgtc gggccgtcga tattctgcgc gagcgcaatg
     3121 atgaactcgc caaactggaa accctcgaca ccggaaaagc gtattcggaa acctcaaccg
     3181 tcgatatcgt taccggtgcg gacgtgctgg agtactacgc cgggctgatc ccgtcgctgg
     3241 aaggcagcca gatcccgttg cgtgagacat catttgtcta cactcgccgc gaaccgctgg
     3301 gcgtggtggc agggattggc gcatggaact acccgatcca gattgccctg tggaaatccg
     3361 ccccggcgct ggcggcgggt aatgcgatga ttttcaaacc gagcgaagtc actccgctta
     3421 ccgcgttaaa gctggctgaa atttacagcg aagcgggcct gccggacggt gtatttaacg
     3481 tgttgccggg cgtgggcgcg gagaccgggc aatatctgac cgagcatccg ggcattgcca
     3541 aagtgtcgtt taccggcggc gtcgccagcg gcaaaaaagt gatggctaac tcggcagcct
     3601 cttccctgaa agaagtgacc atggaactgg gcggtaaatc accgctgatc gttttcgacg
     3661 atgcggatct cgatctcgcc gccgatatcg ccatgatggc aaacttcttc agctccggtc
     3721 aggtgtgtac caatggcacc cgcgtcttcg ttccggcgaa atgcaacgcc gcatttgagc
     3781 agaaaattct ggcgcgcgtt gagcgcattc gcgcgggcga cgttttcgat ccgcaaacta
     3841 acttcggccc gctggtcagc ttcccgcatc gcgataacgt gctgcgctat atcgccaaag
     3901 gcaaagagga aggcgcgcgc gtactgtgcg gcggcgatgt actgaaaggc gatggcctcg
     3961 ataacggcgc atgggttgca ccgaccgtgt tcaccgattg cagcgacgag atgaccatcg
     4021 tgcgtgaaga gatcttcggg ccggtgatgt ccattctgac ctacgagtcg gaagacgaag
     4081 tcattcgccg cgccaatgat accgactacg gcctggcggc gggtatcgtg acggcggacc
     4141 tgaaccgcgc gcatcgcgtc attcatcagc tggaagcggg tatttgctgg atcaacacct
     4201 ggggcgaatc cccggcagag atgcccgttg gcggctacaa acactccggt attggccgcg
     4261 agaacggcgt gatgacgctc cagagttaca cccaggtgaa gtccatccag gttgagatgg
     4321 ctaaattcca gtccatattc taaccaggag gtttatttgc aatttgacta catcattatt
     4381 ggtgccggct cagccggcaa cgttctcgct acccgtctga ctgaagatcc gaatacctcc
     4441 gtgctgctgc ttgaagcggg cggcccggac tatcgctttg acttccgcac ccagatgccc
     4501 gctgccctgg cattcccgct acagggtaaa cgctacaact gggcttacga gacggaacct
     4561 gaaccgttta tgaataaccg tcgcatggag tgcggacgcg gtaaaggcct gggtggatcg
     4621 tcgctgatca acggcatgtg ctatatccgt ggcaacgcga tggatctcga caactgggca
     4681 aaagaacccg gtctggagaa ctggagttat ctcgattgcc tgccctacta ccgcaaggcc
     4741 gagacccgcg acgtgggcga gaacgactac cacggcggcg acggcccggt gagcgtcacc
     4801 acctccaaac ccggcgtcaa tccgctgttt gaagcgatga ttgaagcggg cgtgcaggcg
     4861 ggctacccgc gcacggacga tctcaacggt tatcagcagg aaggtttcgg cccgatggat
     4921 cgcaccgtca cgccgcaggg ccgccgcgcc agcaccgcgc gcggttatct cgatcaggcc
     4981 aaatcgcgcc caaacctaac cattcgtact cacgccatga ccgatcacat tatttttgac
     5041 tgtaaacgcg cggtgggcgt cgagtggctg gaaggcgaca gcaccattcc gacccgcgcg
     5101 acggcgaaca aagaagtgct gttatgtgca ggcgcgattg cctcaccgca gatcctgcaa
     5161 cgctctggcg tcggcaacgc tgaactgttg gccgagtttg atattccgct ggtgcatgat
     5221 ttacccggcg tcggtgaaaa tcttcaggat catctggaga tgtatctgca atatgagtgc
     5281 aaagaaccgg tttccctcta ccctgccctg cagtggtgga atcagccgaa aatcggtgcg
     5341 gagtggctgt ttggcggcac cggcgttggt gccagcaacc actttgaagc aggcggattt
     5401 attcgcagcc gagaggaatt tgcgtggccg aatattcagt atcacttcct gccggtagcg
     5461 attaactata acggctcgaa tgcagtgaaa gagcacggct tccagtgcca cgtcggctcg
     5521 atgcgctcgc caagccgtgg gcatgtgcgg attaaatccc gcgacccgca ccagcatccg
     5581 gcaattctgt ttaactacat gtcgcacgaa caggactggc aggaattccg cgacgcaatt
     5641 cgcatcaccc gcgagatcat gcatcaaccc gcgctggatc agtatcgtgg ccgcgaaatc
     5701 agccccggca cggaatgtca gacggatgag cagctcgatg agtttgtgcg taaccacgcc
     5761 gaaaccgcct tccatccgtg cggtacctgc aaaatgggct acgacgagat gtccgtggtt
     5821 gacggcgaag gccgcgtgca tgggctggaa ggcctgcgcg tggtggatgc gtcaattatg
     5881 ccgcagatta tcaccgggaa tttgaacgcc acgacgatta tgattggcga gaaaatggcg
     5941 gatatgattc gcgggaagga agcgttgccg aggagcacgg cgggatattt tgtggcaaat
     6001 gggatgccag taagagcgaa aaaaatgagt cgtgatttga actgactcag gaaccgatca
     6061 gattgagagt taccgttcca ggtatgggga cagaatcgtt atataaaaca taggggtaac
     6121 tctcatgctt catagtgaca acctcgccag caaattctaa gctgactcgt tcaatctcgc
     6181 tgatgaaggt actatgaatg cactgataaa ccgcaggtga aatgccactc agcttaagaa
     6241 ttacgccgct agtgttcctg gctatggtga cttgttcact cccgctcacg gttagcatat
     6301 acgaaaaggg ttcttgtaac gaggacaaaa a
//
LOCUS       DBFIFM010000233         6327 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.233, whole genome shotgun sequence.
ACCESSION   DBFIFM010000233 DBFIFM010000000
VERSION     DBFIFM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.233"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            51..1487
                     /gene="aspA"
                     /locus_tag="N7C74_004182"
     CDS             51..1487
                     /gene="aspA"
                     /locus_tag="N7C74_004182"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HGU2433939.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            1605..2906
                     /gene="dcuA"
                     /locus_tag="N7C74_004183"
     CDS             1605..2906
                     /gene="dcuA"
                     /locus_tag="N7C74_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HGU2433940.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            3022..3360
                     /gene="cutA"
                     /locus_tag="N7C74_004184"
     CDS             3022..3360
                     /gene="cutA"
                     /locus_tag="N7C74_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HGU2433941.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            3336..5033
                     /gene="dsbD"
                     /locus_tag="N7C74_004185"
     CDS             3336..5033
                     /gene="dsbD"
                     /locus_tag="N7C74_004185"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HGU2433942.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPVDQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLTIVFTLLAMSMFGLLTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGTIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAQLRDRQP"
     gene            5070..5645
                     /gene="yjdC"
                     /locus_tag="N7C74_004186"
     CDS             5070..5645
                     /gene="yjdC"
                     /locus_tag="N7C74_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU2433943.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            5752..5827
                     /locus_tag="N7C74_004187"
     tRNA            5752..5827
                     /locus_tag="N7C74_004187"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5785..5787,aa:Phe,seq:gaa)
     gene            6025..6184
                     /locus_tag="N7C74_004188"
                     /pseudo
     CDS             6025..6184
                     /locus_tag="N7C74_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         1533 a         1634 c         1582 g         1578 t
ORIGIN      
        1 attctgtgtg tttaaagcaa atcattggca gcttgaaaaa gaaggttcac atgtcaaaca
       61 acattcgtat cgaagaagat ctgttgggta ccagggaagt tccagctgat gcctactatg
      121 gtgttcacac tctgagagcg attgaaaact tctatatcag caacaacaaa atcagtgata
      181 ttcctgaatt tgttcgcggt atggtaatgg ttaaaaaagc cgcagctatg gcaaacaaag
      241 agctgcaaac cattcctaaa agtgtagcga atgccatcat tgccgcatgt gatgaagtcc
      301 tgaacaacgg aaaatgcatg gatcagttcc cggtagacgt ctaccagggc ggcgcaggta
      361 cttccgtaaa catgaacacc aacgaagtgc tggccaatat cggtctggaa ctgatgggtc
      421 accagaaagg tgaatatcag tacctgaacc cgaacgacca tgttaacaaa tgtcagtcca
      481 ctaacgacgc ctacccgacc ggtttccgta tcgcagttta ctcttccctg attaagctgg
      541 tagatgcgat taaccaattg cgtgaaggct ttgaacgtaa agcggtcgaa ttccaggaca
      601 tcctgaaaat gggtcgtacc cagctgcagg acgcagtacc gatgaccctc ggtcaggaat
      661 tccgcgcttt cagcatcctg ctgaaagaag aagtgaaaaa catccaacgt accgctgaac
      721 tgctgctgga agttaacctt ggcgcaacag caatcggtac tggtctgaac acgccgaaag
      781 agtactctcc gctggcagtg aaaaaactgg ctgaagttac tggcttccca tgcgtaccgg
      841 ctgaagacct gatcgaagcg acctctgact gcggcgctta tgttatggtt cacggagcgc
      901 tgaaacgcct ggctgtgaag atgtccaaaa tctgtaacga cctgcgcttg ctctcttctg
      961 gtccacgtgc cggcctgaac gagatcaacc tgccggaact gcaggcgggc tcttccatca
     1021 tgccagctaa agtaaacccg gttgttccgg aagtggttaa ccaggtatgc ttcaaagtca
     1081 tcggtaacga caccactgtt accatggcag cagaagcagg tcagctgcag ttgaacgtta
     1141 tggagccggt cattggccag gctatgttcg aatccgttca cattctgacc aacgcttgct
     1201 acaacctgct ggaaaaatgc attaacggca tcactgctaa caaagaagtg tgcgaaggtt
     1261 acgtttacaa ctctatcggt atcgttactt acctgaaccc gttcatcggt caccacaacg
     1321 gtgacatcgt gggtaaaatc tgtgccgaaa ccggtaagag tgtacgtgaa gtcgttctgg
     1381 aacgcggtct gttgactgaa gcggaacttg acgatatttt ctccgtacag aatctgatgc
     1441 acccggctta caaagcaaaa cgctatactg atgaaagcga acagtaatcg tacagggtag
     1501 tacaaataaa aaaggcacgt cagatgacgt gccttttttc ttgtgagcag taacttaaaa
     1561 ataacaatct aatatcaact tgttaaaaaa caaggaaggc taatatgcta gttgtagaac
     1621 tcatcatagt tttgctggcg atcttcttgg gcgccagatt ggggggaata ggtattggtt
     1681 ttgcaggcgg attgggggtg ctggttcttg ccgctattgg cgttaaaccc ggtaacatcc
     1741 cgttcgatgt catttccatt atcatggcgg ttatcgccgc tatttctgcc atgcaggttg
     1801 ctggcggtct ggactatctg gttcatcaga cagaaaagct gctgcgccgt aacccgaaat
     1861 acatcacgat cctcgcaccg atcgtgacct atttcctgac tatctttgct ggtactggca
     1921 acatctctct ggcgacactg ccagttatcg ctgaagttgc gaaggaacaa ggcgttaaac
     1981 cttgccgtcc gctgtctact gcagtggtat ccgcgcagat tgcgatcacc gcatcgccaa
     2041 tctcagcggc agtggtttac atgtcttccg tgatggaagg tcatggcatc agctacctcc
     2101 atctgctctc cgtggtcatc ccgtccaccc tgctggcggt tctggtgatg tccttcctgg
     2161 tcactatgct gttcaactcc aaactctctg acgatccgat ttatcgcaag cgtctggaag
     2221 agggcctggt tgaactgcgc ggtgaaaagc agattgaaat caaatccggt gcaaaaacgt
     2281 ccgtctggct gttcctgctg ggcgtagttg gcgtggttat ctatgcaatc atcaacagcc
     2341 caagcatggg tctggttgaa aaaccgctga tgaacaccac caacgcaatc ctgatcatca
     2401 tgctcagtgt tgcaactctg accaccgtta tctgtaaagt cgataccgac aacatcctca
     2461 actccagcac cttcaaagca ggtatgagcg cctgtatttg tatcctgggt gttgcgtggc
     2521 tgggcgatac tttcgtttcc aacaacatcg actggatcaa agataccgct ggtgaagtga
     2581 ttcagggtca tccgtggctg ctggccgtca tcttcttctt tgcttctgct ctgctgtact
     2641 ctcaggctgc aaccgcaaaa gcactgatgc cgatggctct ggcactgaac gtttctccgc
     2701 tgaccgctgt tgcttctttc gctgcggtgt ctggtctgtt cattctgccg acctacccga
     2761 cgctggttgc tgcggtacag atggatgaca cgggtactac ccgtatcggt aaattcgtct
     2821 tcaaccatcc gttcttcatc ccgggtactc tgggtgttgc cctggccgtt tgcttcggct
     2881 tcgtgctggg tagcttcatg ctgtaatgac caatcgcggg gcgttcacgc cccgctttct
     2941 ttctcgccga ctaacatcct ttccccgtcc gttgtatagt gacctctctc ttgcggttcc
     3001 atctgttctt gcgaggtgtt tatgcttgat gaaaaaagtt cgaataccgc gtctgtcgtg
     3061 gtgctatgta ccgcaccgga tgaagcgaca gcccaggatt tagccgccaa agtgctggcg
     3121 gaaaaactgg cggcctgcgc gaccttgatc cccggcgcta cctctctcta ttactgggaa
     3181 ggtaagctgg agcaagaata cgaagtgcag atgattttaa aaactaccgt atctcaccag
     3241 caggcactgc tggaatgcct gaagtctcat catccatatc aaaccccgga acttctggtt
     3301 ttacctgtta cacacggaga cacagattac ctctcatggc tcaacgcatc tttacgctga
     3361 tcctgctact ttgcagcact tccgtttttg ccggattatt cgacgcgccg ggacgttcac
     3421 aatttgtccc cgtggatcaa gcctttgctt ttgattttca gcaaaaccaa catgacctta
     3481 atctgacctg gcagatcaaa gacggttact acctctaccg taaacagatc cgcattacgc
     3541 cggaacacgc gaaaattgcc gacgtgcagc tgccgcaagg cgtctggcat gaagatgagt
     3601 tttacggcaa aagcgagatt taccgcgatc ggctgacgct tcccgtaacc atcaaccagg
     3661 cgagtgcggg agcgacgtta actgtcacct accagggctg tgctgatgcc ggtttctgtt
     3721 atccgccaga aaccaaaacc gttccgttaa gcgaagtggt cgccaacaac gcagcgtcac
     3781 agcctgtgtc tgttccgcag caagagcagc ccaccgcgca attgcccttt tccgcgctct
     3841 gggcgttgtt gatcggtatt ggtatcgcct ttacgccatg cgtgctgcca atgtacccac
     3901 tgatttctgg catcgtgctg ggtggtaaac agcggctctc cactgccaga gcattgttgc
     3961 tgacctttat ttatgtgcag gggatggcac tgacttacac ggcgctgggt ctggtagttg
     4021 ccgccgcagg gttacagttc caggcggcgc tacagcaccc atacgtgctc attggcctca
     4081 ccatcgtctt taccttgctg gcgatgtcaa tgtttggctt gcttactcta caactcccct
     4141 cctcgctgca aacgcgcctc acgctgatga gcaatcgcca acagggcggc tcacccggcg
     4201 gagtgtttat tatggggacg attgccggac tgatctgttc accatgcacc accgcaccgc
     4261 ttagcgcgat tctgctgtat atcgcccaaa gcgggaacat gtggctgggc ggcggcacgc
     4321 tttatctcta tgcgttgggc atgggcctgc cgctgatgct aattaccgtc tttggtaacc
     4381 gcttgctgcc gaaaagcggc ccgtggatgg aacaagtcaa aaccgcgttt ggttttgtga
     4441 tcctcgcact gccggtcttc ctgctggagc gagtgattgg tgatgtatgg ggattacgct
     4501 tgtggtcggc gctgggtgtc gcattctttg gctgggcctt tatcaccagc ctacaggcca
     4561 aacgcggctg gatgcgcgtg gtgcaaataa tcctgcttgc agcggcattg gttagcgtgc
     4621 gcccacttca ggattgggca tttggtgcga cgcataccgc gcaaactcag acgcatctca
     4681 actttacaca aatcaaaacg gtagatgagt taaatcaggc gctcgttgaa gccaaaggca
     4741 aaccggtgat gttagatctt tatgccgact ggtgcgtcgc ctgtaaagag tttgagaaat
     4801 acaccttcag cgacccgcag gtgcaaaaag cgttagcaga cacggtatta cttcaggcca
     4861 acgtcactgc caacgacgca caagatgtgg cgctgttaaa gcatcttaat gtccttggcc
     4921 taccgacaat tctctttttt gacggacaag gccaggagca tccacaagca cgcgtcacgg
     4981 gctttatgga tgctgaaacc ttcagcgcac aattgcgcga tcgccaaccg tgaacgacac
     5041 ttgcagtggg aaagacggag gaaaatagcg tgcaacgtga agatgtactg ggagaagccc
     5101 tgaaattatt agaattacaa gggattgcca acaccacgct ggagatggtt gctgaacgtg
     5161 tggattatcc actggacgag ctacgccgct tctggccaga caaagaggca atcctctacg
     5221 atgcgctgcg ttatcttagc caacagatag atgtctggcg tcgtcagctg atgctggacg
     5281 aaacgcaaac cgccgaacaa aagctgctgg cacgttatca ggcgttatcg gagtgcgtta
     5341 aaaacaaccg ctatccgggc tgtctgttta tcgctgcctg tacgttttat cccgatcctg
     5401 gccaccctat tcatcaactg gccgatcagc aaaaaagcgc ggcctacgat ttcacccacg
     5461 aactgttaac cacgctggaa gttgacgatc cggcgatggt agcaaagcag atggaactgg
     5521 tgctggaagg ctgtttaagc cgaatgctgg tgaatcgtag ccaggcggat gtcgacaccg
     5581 cacatcggct ggcggaagat atcctgcgct tcgcccgctg ccgccagggt ggtgcactga
     5641 cctgacagaa acacagaaaa gaagcgattt gccgcaatct taagcagttg aatcgctttt
     5701 actgaaatta ggttgacgag atgtgcagat tacggtttaa tgcgccccgt tgcccggata
     5761 gctcagtcgg tagagcaggg gattgaaaat ccccgtgtcc ttggttcaat tccgagtccg
     5821 ggcaccaaat tcatataaac ggacctccac ggaggtccgt ttttcgtttc aggacgccac
     5881 gatttaagtg ttctgcctcc aaatcaattc taccgaactc aacccgattc tccccacatc
     5941 accagcaatt tgcgggcata tcccaattcg ggaaaatttg ttcctgagct atagcactaa
     6001 ctaacgtgaa atgtcgtgcg gccccgtgat gctgttgaac gtcaaatgac gtcatcagga
     6061 gcgtaacgca tccataaagc gcaacatcgg tctgaacgcc gactgatgag attttctgaa
     6121 tgagaacaaa gagaaatgta tcagtccgct tgctcatgca aagactaaca atccattaaa
     6181 atagtaagcg ctccggacaa ttttccatgg attattttct gaacattttt ctttggcaaa
     6241 gatgatgaat tttgatggta aggaatatta cttctggttc tcagtaaaat cctttcgtaa
     6301 taccatgtaa tcaagaagtt tatggct
//
LOCUS       DBFIFM010000234         6297 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.234, whole genome shotgun sequence.
ACCESSION   DBFIFM010000234 DBFIFM010000000
VERSION     DBFIFM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.234"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..910
                     /gene="shuT"
                     /locus_tag="N7C74_004189"
     CDS             <1..910
                     /gene="shuT"
                     /locus_tag="N7C74_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HGU2433944.1"
                     /translation="RIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWAFS
                     VTAAERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEGIL
                     SLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLQQMYANIRQLAKTLQVPEQ
                     GEALVTQISQRLERVQQNVATKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAGAE
                     NVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRIITV
                     DQNLILGMGPRIADVVESLHQQLWPQ"
     gene            930..2267
                     /gene="hutW"
                     /locus_tag="N7C74_004190"
     CDS             930..2267
                     /gene="hutW"
                     /locus_tag="N7C74_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HGU2433945.1"
                     /translation="MNANNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTASPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLSNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMLIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIMREKP"
     gene            2280..2774
                     /gene="hutX"
                     /locus_tag="N7C74_004191"
     CDS             2280..2774
                     /gene="hutX"
                     /locus_tag="N7C74_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HGU2433946.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVRNLPSSTVVPG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHTLAASL
                     KEHA"
     gene            2774..3397
                     /gene="chuY"
                     /locus_tag="N7C74_004192"
     CDS             2774..3397
                     /gene="chuY"
                     /locus_tag="N7C74_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HGU2433947.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVRRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            3446..4438
                     /gene="shuU"
                     /locus_tag="N7C74_004193"
                     /gene_synonym="chuU"
     CDS             3446..4438
                     /gene="shuU"
                     /locus_tag="N7C74_004193"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HGU2433948.1"
                     /translation="MLKDSFSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCAIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEPQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            4435..5205
                     /locus_tag="N7C74_004194"
     CDS             4435..5205
                     /locus_tag="N7C74_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HGU2433949.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCILHDLNLAAHYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(5257..5904)
                     /gene="yhiD"
                     /locus_tag="N7C74_004195"
     CDS             complement(5257..5904)
                     /gene="yhiD"
                     /locus_tag="N7C74_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HGU2433950.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(5968..6294)
                     /gene="hdeB"
                     /locus_tag="N7C74_004196"
     CDS             complement(5968..6294)
                     /gene="hdeB"
                     /locus_tag="N7C74_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HGU2433951.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
BASE COUNT         1563 a         1615 c         1564 g         1555 t
ORIGIN      
        1 aaggatcatc accaggccgt ttcttttttc cccactgacg ttatgtattt cagccgtggc
       61 ctctgccgcg aagaccgcag taaaacgtaa aaaattgttt acggcagtac tggctcttag
      121 ctgggctttt agcgtaacgg ctgccgaacg tatcgtggtc gcaggaggat cgctgacgga
      181 gctaatctac gcgatgggcg ctggcgaacg cgtggttggt gtcgatgaaa cgacatctta
      241 tccaccagaa accgccaaac tgccgcatat tggctactgg aaacaactca gcagcgaagg
      301 cattttgtca cttcgcccgg atagcgtaat tacctggcag gatgccgggc cacaaattgt
      361 gctcgaccaa ctacgagcgc agaaagtcaa cgtcgtcacc ctgccgcgtg tgcctgccac
      421 ccttcagcag atgtacgcca acattcgcca actggcaaaa acgctacagg ttcctgaaca
      481 aggcgaggcg ctggtgacac aaatcagcca acgcctggag cgagtacagc aaaacgtggc
      541 gaccaaaaaa gccccggtta aagccatgtt tattctctcc gcaggcggaa gtgcgccaca
      601 agttgccggt aaaggcagcg tcgcggacgc cattctttcg cttgccggag cagaaaacgt
      661 cgctacccac cagcagtaca aaagctacag tgcagaatca ctgattgcgg ctaatccaga
      721 agtgattgtc gtaacctcac aaatggtcga tggcgatatt aatcggctac gttctattgc
      781 tggaattacc cacactgccg catggaaaaa ccagcgcatt atcaccgttg atcaaaacct
      841 gattctggga atgggtccgc gtattgctga tgtcgttgag tctttacacc agcagctttg
      901 gccgcaataa atggatttaa atcagacgaa tgaacgcaaa taacaccctg gatctcacgc
      961 cacattttgc tttagacggt gatcagccat ttaaagacag gcgcgccatg atgccgtttc
     1021 gtggagccat tccggttgcc aaagagcaac tggcacagac ctggcaagag atgatcaatc
     1081 aaacggcatc acctcgtaag cgactggttt acctacacat tcctttttgt gcgacacact
     1141 gcacgttttg tggtttttat cagaaccgtt ttaatgaaga tgcgtgtgct cattataccg
     1201 acgccctgat tcgtgaaata gaaatggaag cggacagcgt attgcatcag tctgccccca
     1261 tccacgcggt ctatttcggt ggcggtacgc cttccgcact ttcggcacac gatttggcca
     1321 gaatcatcac cacattacga gaaaagctgc cactggcccc tgattgtgaa atcactatcg
     1381 aaggacgggt attgaatttt gacgctgagc gaatcgatgc ttgccttgat gcaggagcaa
     1441 accgcttctc aattggtatc cagtcgttta acagcaaaat ccgcaagaaa atggcccgca
     1501 cctcggatgg cccaaccgct attgcgttta tggaaagcct ggtcaaacgt gaccgtgctg
     1561 cggtggtctg tgacctgctg tttggcctgc cgggtcagga tgcgcaaacc tggggagaag
     1621 atctggctat tgcccgcgat atcggtctcg acggcgtcga tctctatgcg ctcaatgtcc
     1681 tgtccaatac accgctgggc aaggccgtgg aaaatgggcg tactaccgta ccctcaccgg
     1741 cagaacgtcg cgatctttat ctgcaaggat gtgattttat ggacgatgcc ggttggcgct
     1801 gcatcagtaa cagccactgg ggccgtacca cacgcgaacg caatctctat aacctgctga
     1861 ttaaacaagg tgccgattgt ctggcctttg gttccggagc cggcggttcg attaatggtt
     1921 actcctggat gaacgaacgc aatcttcaga cctggcatga atccgtcgcg gcaggcaaaa
     1981 aaccgctgat gttgatcatg cgtaacgccg aacgtaatgc gcaatggcgt cataccttgc
     2041 agtcaggtgt tgaaacagcg cgcgtaccgc tggatgaact tacgccacat gccgaaaaac
     2101 tcgcgccgtt actggctcaa tggcaccaaa aaggcttaag tcgcgatgct tcaacttgcc
     2161 tgcggctgac taatgaaggt cgtttctggg caagcaatat tttgcagtct cttaatgaac
     2221 taattcaggt acttaatgcg ccagcgatta tgcgtgaaaa accataacag gagtgattta
     2281 tgagccatgt ctcgttacaa gaatttttga aaacggagcc ggacggtacg ctggaggtgg
     2341 tagccgaaca atacaacacc acactgctgg aggtagtgag aaatctaccc tcctctaccg
     2401 tagtgccagg cgataaattc gatactgtct gggataccgt ctgcgaatgg ggcaacgtca
     2461 ccacgttagt acatactgcc gatgtaatcc tcgaatttag cggcgaactg ccttcgggtt
     2521 tccatcgcca cggctatttc aacctacgcg gaaagcacgg catgtccggg catatcaaag
     2581 cagaaaactg cacgcatatt gccttaatcg aacgtaaatt tatgggcatg gacaccgcgt
     2641 ccattctctt tttcaacaaa gaaggcagcg ccatgctgaa aatcttcctc ggcagggatg
     2701 accatcgtca gctcctgagc gaacaagtca gcgccttcca tactctggcg gcatcgttaa
     2761 aggaacacgc ctgatgacgc cgtggctact ctttggcgca ggcggaaaag gtgtgggtgc
     2821 cagaacgctt gaactggcgc tggcggaaca acgtccggtc gttgctgtca ttcgtcatgc
     2881 cgatgccgcc acgaaactgg cgcaacaagg cgtacaggtt tttacaggcg acgcctgtga
     2941 tgccagcgta gtcgcggctg cttgccgcgc agctggcccg gatgcgctta tcatctcaac
     3001 aatgggagga gctcaggatt atctggcgca cagaacagtg attgatgagg ccgaaaaggc
     3061 cggcatcagt cgcatgattc tggtgacctc tttgggatgc ggcgatagct ggccgttttt
     3121 atcggagcgg gcaaaggctg cgtttggtca ggcggtacgg gaaaaaacgc tggcggaaag
     3181 ctggctgcaa accagccagc tagactacgc cattctccgc cctggtggtc tgcttgacgg
     3241 cgcagcaaca ggcaaagcac aacggataca aaatcaggag tgtcatggtt ttgtccgccg
     3301 tgccgatgtc gccgcacata tccatgaact ggcaaatgcg ccagcactta accagcaggt
     3361 ctacagtctg attgaaccgg acctgaaacc ggcgtgatac cccggcgcgg catgtccgcg
     3421 ccgtaatgtc gtaacggagt tttgcgtgct caaggattca ttttcatccg ccagggtctt
     3481 tatggggcta tcactattat tgcttgccct ggtgctgttc ggtgctagtc agggagcgtt
     3541 aaagatcagt tttgatgccc tttttgatga ggaataccgc gatatctggc tcaatattcg
     3601 tctaccacgg gttttgctgg cggtgctggt aggtgcagcg ttggcaaccg caggggtaat
     3661 tatgcagggg ctttttcgca acccaatggc tgacccagga ttactcggcg tcagtagcgg
     3721 ttctgcatta atggttggcg ttgcaattgt gctgcccttc tccttcccag tggtgctggt
     3781 gctctatgag caaatggtgt ttgccattgc cggaagttta gtagtctgcg ccatcatttt
     3841 tctcatcacg cagcgccatc gcgatggcag catgatgcaa ttattactcg ccggtatcgc
     3901 catcaatgcc ctgtgcggcg cagcgatcgg catcctgagc tatattggcg atgagccgca
     3961 gctacgacaa ctcacattgt ggatgatggg caatcttgga caggcgcaat ggccgacgtt
     4021 attggttgcc agttcattca tccttccggc cattatcgca acaacttgtc tcgccggaac
     4081 gctgaattta ctgcagctcg gtgatgaaga agcccattac ctcggcgtga acgttaagcg
     4141 taaacgccag caattactat tagtgagctc actgctcgtt ggtgctgccg tatcggtaag
     4201 cggcattatc ggctttattg gcctggtgat cccgcatctg attcgcatga ctaccggagc
     4261 aaatcaccgc tggctcatcc cttgttccgc cctcgccgga gcctgtttat tgctgatggc
     4321 agacacgctt gcccgcacgc tggtacagcc agcagaaatg cccgtgggat tattaaccag
     4381 cctgcttggt ggcccttatt ttatgtggtt gattctgcga aatcggagga tcacatgatc
     4441 agcgcccaga atctggttta tagccttcag gggcggcgtc tgactgataa tgtctcgctg
     4501 acttttcccg gtggggaaat tgttgccatt ctcggcccta atggtgcggg aaaatctacg
     4561 ctgcttcgcc agttaaccgg ctatcttcaa cctgatagcg gcgaatgtcg gttgtttaat
     4621 aagccattaa atgaatggtc gattactgaa ctggcgaaac accgggccgt gatgcgtcaa
     4681 aatagccaca tggcgtttcc attcagcgta caagaagtta tccagatggg tcggcatccg
     4741 catcgtactg gtaatcagga taatgaaaca gcacaaataa tggcgttatg tgactgtcag
     4801 gcgttagcaa accgcgacta tcggcaatta tccggtggtg aacaacagcg cgtgcaatta
     4861 gcgcgcctgc tggtgcaact ctgggaacca actccctcac cgaaatggtt atttcttgat
     4921 gaaccaactt cggcgctgga tattcaccat cagcagcact tgtttcgttt attacgccag
     4981 ttagttcacg agcggcagtt caatgtctgc tgcatattgc acgatctaaa tcttgccgcg
     5041 cattatgcag accgtgttgt attaatgcaa aaaggaaagg ttatcgcaaa tggtaaacca
     5101 caggacgtat tgacacagca ggcgctgaca atgctctacg gcgcggatat taccgtactt
     5161 aaagatcctg ccaaccattc accactgatc gtcctcgatc attaaaagca acaggagtac
     5221 cgcactggta ctcctgcgaa taaataacta tacagattag ctaattgaaa cgcctttcac
     5281 ccctgccata ccttttaata atcgcaacag atcttctatc gacgttgtgg catgaagctg
     5341 gatatcaatg gcgaccactt catgatcttc attttcctgc aacgaaacta aatcagtttt
     5401 tattttttgc tgcttaaacc agtcgagcat tgataccgta ttaccgttca ctaatgtcag
     5461 ctgtaaatgg taatttttgt tcatcaggcg gaaggttaat tgatggaaga cttccagcac
     5521 cagcaaggtc atcactgaac cataaatacc cagttcgtac ataccactgc caataaccat
     5581 acctatggcg gcggtcaccc agatatccgc tgccgtcgtc aaacctacaa tattttggtt
     5641 acgaacgaga atgttacctg caccgataaa cccgacgccc gtcaccacct gagcagcaat
     5701 acggctgggg tccagtccga cgtgatctaa agacagcacg tcagcaaaac catatttcga
     5761 aacaatcata aacagtgcgc ttcccatgcc aattaataca tgagtgcgta accctgctcc
     5821 tttgccgcgc atttgccttt ccatgccaat agcgccacag gcaattgccg cgagtataag
     5881 acgtataata aattctgctg tcatataaaa atgcaaaaag gagcagcaag atggctcaac
     5941 ttgccactcc tttttacttg tacctcatta attcggcaag tcattagatg cttgattttt
     6001 gaaggtatac aaatttttct gcgggttttt cttacagtat tcgatcactt taggaatttg
     6061 agtgagatcg gtttcattta aagtaactgt atcgccacct ttatatactg tttcttcatg
     6121 cagcatccac catgcaaccg gggtcattgc ttttggattc agatcaataa attcctggca
     6181 ggtcatatct ttagcggatt cattggctgc cagcgcagat tgtgcgttca ccagtgacaa
     6241 agccgctaca gcgcccataa aaataaacgc tttacggaga gatgaaatat ttatttt
//
LOCUS       DBFIFM010000235         6252 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.235, whole genome shotgun sequence.
ACCESSION   DBFIFM010000235 DBFIFM010000000
VERSION     DBFIFM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.235"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..231
                     /gene="paa"
                     /locus_tag="N7C74_004197"
     CDS             <1..231
                     /gene="paa"
                     /locus_tag="N7C74_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HGU2433952.1"
                     /translation="ENQPGADVWITWADWAASNPEIGDVVEIAPDYVIWRDMNITVRQ
                     DANDETRRFAEWLQTDEAAPAFKKYGWTRKGT"
     gene            567..722
                     /gene="hokD"
                     /locus_tag="N7C74_004198"
     CDS             567..722
                     /gene="hokD"
                     /locus_tag="N7C74_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGU2433953.1"
                     /translation="MKQQKAMLIALIVICLIVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            943..1200
                     /locus_tag="N7C74_004199"
     CDS             943..1200
                     /locus_tag="N7C74_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433954.1"
                     /translation="MRIEELREIFSEDGLYTVRVEKGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFILRANEFVTSLPSLKEAGIKAGFSAFED"
     gene            1270..1548
                     /locus_tag="N7C74_004200"
     CDS             1270..1548
                     /locus_tag="N7C74_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433955.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWFRHAPCTEEQAEW
                     LIQNYRRRGYEFRKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            1550..2596
                     /locus_tag="N7C74_004201"
     CDS             1550..2596
                     /locus_tag="N7C74_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU2433956.1"
                     /translation="MRVLLRPVPVPELGVVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGIVPAVRQPLAEDKSLLPFFSNERVIRAVGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATIILKQR
                     TKNLAPLPHAHQQNPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
     gene            2609..2968
                     /locus_tag="N7C74_004202"
     CDS             2609..2968
                     /locus_tag="N7C74_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU2433957.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVVLIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYKIESE"
     gene            2977..3507
                     /locus_tag="N7C74_004203"
     CDS             2977..3507
                     /locus_tag="N7C74_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HGU2433958.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRSMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            3749..3946
                     /locus_tag="N7C74_004204"
     CDS             3749..3946
                     /locus_tag="N7C74_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGU2433959.1"
                     /translation="MLKQQDMTETARVVFNELSVTDPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            4097..5155
                     /locus_tag="N7C74_004205"
     CDS             4097..5155
                     /locus_tag="N7C74_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HGU2433960.1"
                     /translation="MLNTVKISSCELINADCLEFMRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWAGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDTEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRTLKQHVMA
                     PLIAYFRDARAVLGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVDTYASLNRQYAELQSEYKHLRRYFGVTVQVPYTDVWTYKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLIIRNG"
     gene            5196..5271
                     /locus_tag="N7C74_004206"
     tRNA            5196..5271
                     /locus_tag="N7C74_004206"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5229..5231,aa:Ile,seq:cat)
     gene            5352..5428
                     /locus_tag="N7C74_004207"
     tRNA            5352..5428
                     /locus_tag="N7C74_004207"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5386..5388,aa:Arg,seq:tct)
     gene            5952..6044
                     /locus_tag="N7C74_004208"
                     /pseudo
     CDS             5952..6044
                     /locus_tag="N7C74_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001455460.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
     gene            6035..6252
                     /locus_tag="N7C74_004209"
                     /pseudo
     CDS             6035..6252
                     /locus_tag="N7C74_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1581 a         1446 c         1751 g         1474 t
ORIGIN      
        1 gagaatcagc ctggagcaga tgtctggata acctgggctg actgggcagc cagtaatcca
       61 gaaattggtg atgtcgtgga aatagcgcca gactacgtga tatggcgtga tatgaacatt
      121 acagtacgtc aggatgcaaa tgatgaaacc cgtcgatttg cagaatggct acaaaccgat
      181 gaagcggcgc ctgcattcaa aaaatatggc tggaccagga aaggcacttg acatcctcgt
      241 ccttcaggac gtggattctt tttccggatg ccgcgccagc ggcatgtagg ggcagctcac
      301 aaaacggaaa aaattgtacg ctaagcctcg ccaggtgaac tgaattcatt ccgatatggg
      361 aattcccata tcgggcgaaa acggtttgct gtaacggcag agttaagtag gattgctgcg
      421 ggtgcttgag gctatctgcc tcgggcatga acaccaacgg cagatagata aaagccccac
      481 ccgactataa atcgaagtga ggcccctata tgctcgtcac atatagattg cctcttacgg
      541 accgaaaggt caaggagaag caggctatga agcagcaaaa ggcgatgtta atcgccctga
      601 tcgtcatctg tttaatcgtc atagtgacgg cactggtaac gaggaaagac ctctgcgagg
      661 tacgaatccg aaccggccag acggaggtcg ctgtcttcac agcttacgaa cctgaggagt
      721 aagagaccag gcgggggaga aatccctcgc cacctctgat gtgtcaggca tcctcaacgc
      781 acccgcactt aacccgcttc ggcgggtttt tgtttttatt ttcaacgcgt ttgaagttcc
      841 ggacggcgcc ggaatagaat caaaaatact taagtagcgc gcagggagaa gagggatgga
      901 ccccgaacag gggagtgcta tttatctgga aggattctgt tgatgagaat cgaagaatta
      961 cgtgaaattt ttagtgaaga tggcctctat actgtgcgcg ttgagaaggg cgctattgtc
     1021 agccactgcc gtattaaatg tttacagtct caacaaagga agagtggagc tgcgttaatt
     1081 cattttgtgg atgggcttgt gacggatggt tttattttgc gtgcaaatga atttgtcaca
     1141 tcgttgccgt ctctgaaaga agctgggatt aaggctggtt tttctgcttt tgaagattag
     1201 tgaattcatc tacaattcag cgcagggctg aacccctgtt gagtaacact gtgccaccgg
     1261 agaaagccga tggcgcaaaa ttccagacta cacaattctg ataattcagc cgtctttgcc
     1321 agcaggcacg ggcggcgttc tcatgcattc aaatctgact ggttccggca cgccccatgc
     1381 actgaagaac aggccgaatg gctgattcag aactaccgca gacgtgggta tgagtttagg
     1441 aaagccctca gcctcgatta tcgtcactgg ataatctacg tcaggctgcc gtactccgag
     1501 cgcccaccgc gtccgtcccg cacattccag caacgcatct ggaggtaacg tgcgggtatt
     1561 acttcgacct gttccggtac cggaacttgg ggtggtggtc cttaaaccag gccgtgaatc
     1621 catgcaggta ttccataacc ctcgagtgct tgtggagccg gagccgaaaa gcatgcgcgg
     1681 tctgccgtcc ggcatcgtcc ctgccgttcg ccagccgctg gcggaggata aatcattact
     1741 gccgtttttc agcaatgagc gtgtgattcg tgctgttggt ggcgctggtg cactgtctga
     1801 ctggctgttg cgtcatgtca aatcctgcca gtggcctcat ggtgactatc atcacagtga
     1861 aaccgttatt caccgttatg gtaccggcgc aatggtgttg tgctggcact gcgacaacca
     1921 gctgcgtgac cagacatccg aatcactcga gcaacttgct catcaaaacc tgtcagcatg
     1981 gatgattgac gtcatcggtc acgcaataag cggtacgcag gagcgtgaat tatctctggc
     2041 tgaattatcc tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg aagcggtatt
     2101 acgtcgttcg ctggggttgc gtgcggaaaa aatccgctca atgtaccgtg aaagcgacat
     2161 cgtaccggga gagcagaccg ccaccatcat actgaagcag cgcacaaaaa atcttgcgcc
     2221 gctgcctcac gcccaccagc aaaacccacc acaggaaaag acggtggtca gcattgccgt
     2281 tgatccggag tctccggaat ccttcatgaa acgacctaaa cgtcgccgct gggtaaatga
     2341 gaaatacaca cgctgggtaa agacacagcc gtgtgcgtgt tgtggtaagc cagcggacga
     2401 tcctcatcat ctgattggtc atggtcaggg cggaatggga acaaaatccc acgatatttt
     2461 cacgctaccg ctgtgtcggg agcatcacaa cgagcttcat gcggatccgc tggcgttcga
     2521 agaaaagcat ggttcccagg ttgatttaat ttttcgtttt cttgatcacg cctttgcgac
     2581 tggcgtgctc gggtaaaaga ggtgactgat gctcatagat ttggttttac cttacccgcc
     2641 gacggtgaac acttactggc gacgccgtgg cagcacatat tttgtatcaa aagccgggga
     2701 gcgttatcgc cgggcagtgg tgcttattgt tcgccagcag cgactgaaat taagcctgtc
     2761 cggacggctg gcaataaaaa ttattgcaga gccaccggat aagcgtcgtc gtgacctgga
     2821 caatattctg aaagcaccgc tggatgcgct gacgcatgcg ggagtgttaa tggacgatga
     2881 gcagtttgat gaaatcaata ttgtacgtgg tcagccagta tctggtggac ggctgggtgt
     2941 gaagatttac aaaattgaga gtgagtgagc gtaaatatga tatatccgga aattacaggc
     3001 aaaagcggcg aacatttacg cctgaacacg ctggaagcag tctggatcca ggggaaatta
     3061 cggatgtggg ggcggtggtc gtatatcggt gggggtaaat ccggaaatat gtttaaccgg
     3121 ttactggttt cgaaaaagct gacgaaaaca gcagttaatg aggttttacg cagcatgaag
     3181 aaatccgggc tggaaaaacc ggaacttgag gcattttttc gggatatgac cagagggaag
     3241 cagaagagct ggttgtcaca ttgtacagac acagaggcgt tgattattga tcgcgttatc
     3301 agtgaggtgc ttggggaata tcccgggcta atcaatattc tccggcaaag gtacgaagga
     3361 cggggaatga gtaagagaaa aatggcagaa tgtttaaatc gtactcaccc ggaatggtgt
     3421 ttcagcacat gtgagaaacg tattgcaggt tggttagccg tggctgaaca catgctttat
     3481 gtacctatgc acgattcatt tcgataaaaa aagcttgctt ttttacgcag aaacagcttg
     3541 aattcctgta agcttcgcaa agctgtatcg cgaggcgaaa tgcaagtttt ttcgcacaag
     3601 gaagccaccg gaaggtggtt tttttgtgtc cgtaatatac agcagcgcaa taaattcgct
     3661 ggtggttatt aataccgttc tttcaggttg ctggcttttt cgacaagagt tattggtgtg
     3721 tcacgttaac cggaaaaggg aaaaagacat gctgaaacag caggatatga cagaaaccgc
     3781 cagagtggtg tttaatgaat taagcgttac cgacccggcg acagtcgggg agattgcgca
     3841 gaatacttac ctttcacgcg aacgctgcca gttaatactg acccagctgg ttatggcggg
     3901 tctggcagac tatcagtttg gttgttacag acgccttcag tcctgaaggc ttttttattt
     3961 gtggtaaatg ggcggctggt gggtgtaagg ggcacccacc agccatctgc tcatgcgttg
     4021 ggttcacaag caaacctcag gcccactgct ttgcgcaaaa gcagaatgag cctatcagag
     4081 acaggcttaa tgatccatgc ttaatactgt aaaaatatcc agttgtgagt taatcaacgc
     4141 cgactgcctg gaatttatgc ggtcgttacc cgaaaattct gttgacctga tagtcacgga
     4201 cccgccgtac ttcaaagtga aacccgaggg ctgggataac cagtgggcgg gtgatgaaga
     4261 ttacctgaag tggctggacc agtgtcttgc gcagttctgg cgggtgctga aacctgccgg
     4321 aagtctttac ctgttctgtg gccatcgtct ggcatctgac accgaaatca tgatgcgtga
     4381 gcggtttaac gtgctgaacc atatcatctg ggcaaagccg tccggacgct ggaacgggtg
     4441 caacaaggaa agcctgcggg cgtatttccc cgccacagag cgcattctgt tcgcagagca
     4501 ttatcagggg ccgtatcgtc cgaaagatgc cgggtatgag gcgaagggta ggacactgaa
     4561 acagcatgtg atggccccgc tgattgctta ctttcgtgat gcgcgcgctg tcctggggat
     4621 aacggcaaaa cagattgcag atgccacagg aaagaaaaac atggtgtcgc actggttcag
     4681 tgccggtcag tggcagctgc cgaacgaaag cgattatctg aaattacagg cactgtttgc
     4741 ccgggtggca gaagagaagc atcagcgggg tgaactggaa aagccccacc accagctggt
     4801 ggatacgtat gcctctctga accgacagta tgcggagctg cagagtgaat ataagcatct
     4861 gcggcggtat ttcggtgtga cggtgcaggt gccgtacacc gatgtgtgga cgtataaacc
     4921 ggtgcagtac tatccaggga aacatccgtg cgaaaaaccg gcagaaatgt tgcagcagat
     4981 aatcagcgca agcagtcgtc cgggagacct ggttgcagat ttcttcatgg ggtcggggtc
     5041 gacagtgaaa gcagcgatgg cgctgggacg tcgtgcaact ggcgttgaac tggagactga
     5101 acgttttgag cagacggtgc gggaagtaca ggatttaatc attcgtaacg gatgagattg
     5161 cggagttaat catgcgtcgt tattattcag caatcggccc tttagctcag cggtgagagc
     5221 gagcgactca taatcgccag gtcgctggtt caaatccagc aagggccacc aaccgccact
     5281 agctcatcag gaaagaacgt caccctgtgc gagattcgga gtccccggtg gcggtccatt
     5341 atcggtattc tgcgttgtta gctcagccgg acagagcaat tgccttctaa gcaatcggtc
     5401 actggttcga atccagtaca acgcaccaca ccacacttat ctgccctgac tctcttttgc
     5461 gggcttttta ttacaggaaa gacaccggac agtgaaatgt taaatgcctc acaattcagg
     5521 cagttgactg ttgcctgaca tgctgagcgt ttgttaaaaa aatcctgcat gatgaatccc
     5581 cctgagcggc ggggcataat gacagatgtt tggttgcgta ttgtataggc aagttgcgga
     5641 ttctgtctgg tcattgcaga attcaccggg aggcacccgg catcatgctg tatacagaga
     5701 ttaggcatat atccaggctt ctcatcgcag gagccttttt acatgcaaaa aaaagcccga
     5761 gtgggttcgg gcaacagcat gagatacttg cattgtcatt tttatcgtgt ggattttaac
     5821 cagggtttat aaggctgcgc aactgcgcgg cctttttcgt tttgcgggct gcggttctcc
     5881 tcttttgatt ctccttgtgg ccggaccgtg gcccgcaact gttgaggaaa atcccggaaa
     5941 ggggaggaat aatgacattt aaacattatg atgttgtcag ggcggcgtcg ccgtcagacc
     6001 ttgcggaaaa gctgacacac aaactgaaag agggctggca accgctattc tgaacgcagc
     6061 cgggcgcacc tcagccttca tcagtggtaa ggcaccggaa atcaaaccct cgcccggcgg
     6121 caacacgcca tcgggtccgt ctgcagatac gtccgttcgc acaatctccc tgctgccggc
     6181 agccggagag gctgctgcgc agggctggag cattaaggat ggcggaattc agttgtcaga
     6241 tggtgtattt aa
//
LOCUS       DBFIFM010000236         6167 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.236, whole genome shotgun sequence.
ACCESSION   DBFIFM010000236 DBFIFM010000000
VERSION     DBFIFM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.236"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            25..1485
                     /locus_tag="N7C74_004210"
     CDS             25..1485
                     /locus_tag="N7C74_004210"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HGU2433961.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLHQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GGDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1521..1724)
                     /gene="ydfZ"
                     /locus_tag="N7C74_004211"
     CDS             complement(1521..1724)
                     /gene="ydfZ"
                     /locus_tag="N7C74_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HGU2433962.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1901..2587)
                     /gene="rspR"
                     /locus_tag="N7C74_004212"
                     /gene_synonym="ydfH"
     CDS             complement(1901..2587)
                     /gene="rspR"
                     /locus_tag="N7C74_004212"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HGU2433963.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2676..3422)
                     /gene="ydfG"
                     /locus_tag="N7C74_004213"
     CDS             complement(2676..3422)
                     /gene="ydfG"
                     /locus_tag="N7C74_004213"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HGU2433964.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASIEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3559..5604
                     /gene="dcp"
                     /locus_tag="N7C74_004214"
     CDS             3559..5604
                     /gene="dcp"
                     /locus_tag="N7C74_004214"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HGU2433965.1"
                     /translation="MTTINPFLVQSTLPYLAPHFDQIANHHYRPAFDEGIQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5649..>6167)
                     /locus_tag="N7C74_004215"
     CDS             complement(5649..>6167)
                     /locus_tag="N7C74_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HGU2433966.1"
                     /translation="VNINRNKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1532 a         1486 c         1579 g         1570 t
ORIGIN      
        1 catatttaaa tttaaggaat tgttatggga aataatttgt tatcagcaaa agcgacactc
       61 cctgtttatg atcgtaataa cctggcccca agaattgttc atttaggctt tggtgcattt
      121 caccgtgcgc atcagggcgt gtatgccgat attcttgcta cagaacattt cagtgactgg
      181 ggatattatg aggtcaactt aatcggcggc gaacagcaaa ttgccgattt acatcagcaa
      241 gataatcttt ataccgttgc ggaaatgtcg gccgatgcgt ggacggctcg cgtcgttggc
      301 gtcgttaaaa aagccttgca cgtacagata gatggcttag aaaccgtgtt ggctgcgatg
      361 tgtgaaccgc aaatcgcgat tgtctctctg acaatcaccg aaaaagggta tttccactct
      421 ccggcgaccg gacagttaat gctcgatcac ccgatggtcg ctgccgacgt gcaaaatccc
      481 caccagccga aaacagcaac aggggtgatt gttgaggcgc tggctcgccg taaagcggca
      541 ggacttcccg catttaccgt catgtcatgt gacaacatgc cagaaaacgg tcatgttatg
      601 cgtgacgttg tcacttccta cgcacaagcc gttgatgtaa aactagcaca atggatcgaa
      661 gataacgtga ctttcccatc aacaatggtg gaccgtattg tgcccgcagt gacagaggat
      721 acgctggcga aaatcgaaca gcttaccggt gtgcgcgatc ctgctggcgt tgcctgtgaa
      781 cctttccgcc agtgggtaat cgaagataac tttgttgccg gacgtccgga atgggaaaaa
      841 gcgggagccg aactggttag tgatgtgctg ccttatgaag agatgaagtt gcgcatgctc
      901 aacggcagtc attcattcct ggcgtatctg ggttatcttg ccggatatca gcacattaat
      961 gactgtatgg aagatgaaca ttatcgtcat gcggcgtatg ccttgatgtt gcaggaacaa
     1021 gcgccgacgc tgaaagtgca gggcgttgat ttgcaagatt acgctaaccg attaattgca
     1081 cgctatagca acccggcgtt acgtcatcga acctggcaga ttgcgatgga tggcagccag
     1141 aaattgccac agcggatgtt ggattctgtt cgctggcatc tggcgcatga cagcaagttc
     1201 gatctgctgg cgctgggcgt cgcgggttgg atgcgttatg tcggtggtgt tgatgaacag
     1261 ggaaatccga tagaaatcag tgacccactg ttacctgtta ttcagaaggc tgtacaaagt
     1321 agtgccgaag ggaaagcgcg cgtccagtca ttgctggcga ttaaggcgat ttttggtggt
     1381 gatttgccag acaatagctt gtttactgca aaagtgacgg aagcgtactt gtctttatta
     1441 gcgcatggtg cgaaagcgac cgtggcgaaa tattccgtga agtaaaaaaa gcaatgccgc
     1501 catcggcggc attcacacgc ttagttcatg ccgagacgaa tcaggtccag tggtgccagt
     1561 ttctcttcac aaccttcaac aactacggtt tttccgcggc ggatttgctc agcggtcaga
     1621 ccttcagtat caatcgacag gatcttgcca gtgtgaccgg tgccgctaac cataacacgg
     1681 ctgccagtgg tgattgcgtt acggttacga tcgtaggtcg tcataggtgt tttctccttt
     1741 ctgatttaca gtcgaagccc acgtaacggg cgcaatataa atactcctgt cgagtgaaat
     1801 attttttgtt tttgatcaag ctcacatctt ttttgatgca tgcatgagcc ataaggcggg
     1861 ggaaaagact ctcaccccgg atgggatgag aggaaatgaa ttactcttcg ctaaaccagt
     1921 cgctattttc ctggcggatc tggcgcacgg attcgctgat ttcctgcaaa tgttgcgtca
     1981 ttgcacgttc taccgcatcg ccatcacgtt tttgcagggc agagaatata tcaagatgct
     2041 ggcgtaacag catttctggt ggagaaacat ggtcgaaact catatagcgc acgcgatcaa
     2101 cggtcgcttt caggttctca atggtatccc acgccagttg acagtcggca atctgcgtca
     2161 ggagttgatg gaagttgtca tcaagttcaa aaaaatcatc cagttgcttg cgctcaatgg
     2221 caatgcgttg ctggtgaaga ttttgttcca gttggtagca ctggctttcg gtaatcatac
     2281 tcgccgcccg acgcgccacc gcgcactcaa tggcctgacg gataaaactg ccgttgcgca
     2341 cctgggccat ggaaattttg ttgacgtagc tgccacgctg cggacgaatt tgaatcaggc
     2401 cgttttccgc cagtttaata aaggcttcac gaaccggctg gcgtgacaca ttgaaacgaa
     2461 cagaaacttc tttttccgac aacggtgtgc ctggtgcaat caggcaatgg acaatgtcgc
     2521 gacgaagaat acgataaatc tgctgattga caggctgtgt gggattaagt tgcgtttcga
     2581 cggtcattcg ttcttgcttg tgagtgagtt aactgcatga gtctaccact tttttgtgac
     2641 aagcaataac ccggcagtta caccgggtat aaaaattact gacggtggac attcagcccg
     2701 gcatagcttt gggtaaccgg catcatttcc agggtattga tattgacgtg agcaggcagc
     2761 gttgacaccc accagacggc ttcgctgaca tcttctggcg ttaatgcaac ggtattttga
     2821 taggtttttt ccgctttacc gtcatcgcct ttaaagcgga cattggaaaa ctcggtgcca
     2881 cccaccagac ccggttcgat gtcggtgacg cgcaccgccg taccatgcag gtccgtacgc
     2941 agattcaggc taaactgacg aacaaacgct ttcgtcgcac cgtaaacgtt accaccggca
     3001 tacggccagc tacctgccgt tgagccaatg ttaataatat gaccatgatt acgttcaacc
     3061 atacccggta agacggcgcg cgtcatatat accaggcctt tgttgttggt atcaatcatc
     3121 gtttcccagt cttcaatgct ggctttatgc gcaggctcca tgcccaacgc cagaccggca
     3181 ttatttacca ggatatcaat attgcaccac tcggcaggaa gcgatgccag catctcttca
     3241 atagcggcgc ggttgcgaac gtccagttgg gcgatgtaca gattatctcc cagttcgtct
     3301 tttaactcct gcaaccgttc ctggcgacgg ccagtggcga taactttatg cccttgttga
     3361 ataaaacgac gagtaatgca ttcaccaaaa cctgccgttg ctccagttac taaaacgatc
     3421 atctcactgt tcctcaacgc ttttgtgtgt caataacata gcacgtgatg cttaagagcg
     3481 ggtaatactg gtttgcaaga ttgcactccg ttatcagtaa gcctacgcta tggaacacct
     3541 cgcgttcagg agaagaaaat gacaacaatt aatcctttcc ttgtgcaaag cacactgccg
     3601 tatctggctc ctcattttga tcaaattgcc aatcatcact atcgcccggc attcgatgag
     3661 ggaatacagc aaaagcgggc agaaattgct gccatcgcgc ttaacccgca aacacctgat
     3721 ttcaacaata ctattctggc actggaacaa agcggagaat tacttacccg cgttaccagc
     3781 gtcttttttg cgatgactgc ggcgcatacc aatgatgaat tacagcgtct tgacgagcag
     3841 ttttccgctg aactggcgga actggctaat gatatctatc tgaacggtga attattcgcg
     3901 cgggtagatg ctgtctggca acgccgtgaa tctctggggc ttgatagtga atccatccgc
     3961 ctggtggagg tgattcatca acgttttgtc ctagccggag ccaaacttgc acaagctgat
     4021 aaagcaaaat taaaagtact gaatacagaa gctgcgacac tgaccagtca gtttaaccag
     4081 cggttactgg cagcaaataa atccggcggt ctggttgtga acgatatcgc gcagctggca
     4141 ggaatgagtg agcaagagat tgcgctggcg gcagaggcgg ctcgcgagaa aggtctggat
     4201 aacaaatggc tgattccgct gctgaatacc acccaacaac cggcgcttgc cgaaatgcgc
     4261 gatcgtgcga cgcgtgaaaa actgtttatt gcgggctgga cgcgagcgga aaaaaatgat
     4321 gccaatgata cccgcgctat cattcaacgt ctggtggaga tccgtgcaca acaggctaca
     4381 ttactcggtt ttcctcatta tgccgcatgg aaaatcgccg atcagatggc aaaaacacct
     4441 gaagcagcac tcaactttat gcgggaaatt gttccagcgg cgcgtcaacg tgcgagcgat
     4501 gaattagcct ccatacaggc ggttattgat aagcagcagg gcgggtttag cgcgcagccg
     4561 tgggactggg cattttatgc cgaacaggtc cggcgagaga aatttgatct tgatgaggcg
     4621 cagctcaagc catattttga attaaacacg gtgttgaatg aaggtgtatt ctggaccgcg
     4681 aatcagctct tcggtattaa gtttgtcgaa cgttttgata ttcctgtcta ccatcctgac
     4741 gttcgggtgt gggaaatttt tgatcataat ggcgtggggc tggcgttatt ttacggtgat
     4801 ttcttcgccc gtgattcaaa aagcggcggt gcatggatgg gcaattttgt tgagcaatca
     4861 acgcttaatg aaacgcatcc ggtaatttat aacgtctgca attatcagaa acccgctgcc
     4921 ggtgagcctg cgttgttact ctgggatgat gtcataacct tattccatga atttggtcat
     4981 acgctgcacg gcctttttgc ccgccagcgt tatgccacgc tctccggcac caacacgccg
     5041 cgtgattttg tcgaatttcc gtcgcaaatc aacgaacact gggcaacgca tccgcaggta
     5101 ttcgctcgct acgcccggca ttatcagagc ggggcagcaa tgcctgacga actgcaacag
     5161 aaaatgcgta atgccagcct gttcaacaaa gggtatgaga tgagcgaact gcttagcgcc
     5221 gcacttctcg atatgcgctg gcattgcctg gaagaaaacg aagcaatgca ggatgtcgat
     5281 gattttgaat tgcgggcgct ggtggcagaa aatatggatc ttcctgctat accgccacgc
     5341 tatcgcagca gttattttgc ccatattttt ggtggcggat atgccgcagg ttattacgct
     5401 tatctgtgga cgcaaatgtt ggccgatgat ggttaccagt ggtttgttga gcagggcgga
     5461 ttaacgcgtg aaaatgggct gcgttttcgc gaggcgatcc tttccagagg taacagcgaa
     5521 gatctggaac gcctgtatcg acaatggcgc ggtaaggctc ctcagattat gccgatgctg
     5581 caacatcgtg gcttgaacat ataaacacca ccaatggctg tcctggagat atcgccaggc
     5641 cagcctggtt atagcagtaa ctgcagcagc tgggcgaggg caaacctcac cgctaaagct
     5701 aataccgttt cgcagtcgcc agcaaaatgc ataaccgcag tgtagttctg gcctttaata
     5761 tgccacgcga accagacggt acccgctggc gtaccatctt caccgccctc cggtccgccg
     5821 tagccggtaa tggcaatact gacatcagca tccgcacgct ctatggcacc ggttgccatt
     5881 tctgctgcca ctttctcact caccgcagaa tatcgttcaa gagattgctg gcttacgctg
     5941 aggattttca tctttgcctg atcggtgaaa gtaacaaagc ctgcaccgta aaatttgggt
     6001 gtatcttcag ctgcacacag ggcgctagcc aacttaccgc cggtgcatga ttcggcggta
     6061 gttaagcgta attgatccgc gataagtctt tgactcaacg cggatgtcag gttttcaata
     6121 gtgtcggtat ctgcaagttg tacaatttta tttctattaa tgttcac
//
LOCUS       DBFIFM010000237         6138 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.237, whole genome shotgun sequence.
ACCESSION   DBFIFM010000237 DBFIFM010000000
VERSION     DBFIFM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.237"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            156..1028
                     /locus_tag="N7C74_004216"
     CDS             156..1028
                     /locus_tag="N7C74_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HGU2433967.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTDAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELCTESVWGQAREQFTGAVDRIFDTVESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            1400..4249
                     /gene="cah"
                     /locus_tag="N7C74_004217"
     CDS             1400..4249
                     /gene="cah"
                     /locus_tag="N7C74_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding autotransporter Cah"
                     /protein_id="HGU2433968.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVFGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGLARDTLIYAGGDQSVHGRALNTTLNGGYQYVHRDGLALNTVINEGGW
                     QVVKAGGAAGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVIGTNRLGNFTV
                     ENGKADGVVLESGGRLDVLESHSAQNTLVDDGGTLAVSAGGKATSVTITSGGALIADS
                     GATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPNAALSRAVAKSNSPVTFH
                     KLTTTNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYT
                     SMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGS
                     YGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLN
                     LVHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQ
                     LQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSA
                     KHSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEAR
                     IRENITLGVQAGYAHSVSGSSAEGYNGQATLNMTF"
     gene            4360..>6138
                     /locus_tag="N7C74_004218"
     CDS             4360..>6138
                     /locus_tag="N7C74_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HGU2433969.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENGLL
                     FDKRKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQSLQQTGDTRELL
                     DILADDVWLKNEYHGEDEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVAF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEMMMRDQLQKASAVLAVMDYTQMNSKADE
                     EVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGALPVDEAWVDDFIREAFGPMVEEDDWKDSTKVNKKAEKL
                     WNISLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QAHITSLLADIQEIEECQNQVTGDVRMAMEDINTKTGELLTKVCASLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRRAALE
                     LMTQIESTVTSLHREAEAQFRPELEKI"
BASE COUNT         1526 a         1567 c         1760 g         1285 t
ORIGIN      
        1 ataccactac tcaactatta agaacaaaat tcgggatgta tgattatcag aagatacctg
       61 catcctaaat aatttcagac agatattatc gttgtgaatc ccccgccacc tttccggtgc
      121 gcggggtttt gtcttttttc accgggaata catgtatgaa tccgtctgat gccattgagg
      181 caattgaaaa accgctctcc tccctgcctt actcgctttc ccgtcacatc ctggaacatc
      241 tgcgcaaact caccagtcac gaacccgtga ttggcattat gggtaaaagc ggggccggta
      301 aatcctcact ctgtaatgca ctgtttcagg gggaggtcac cccggtcagt gatgttcacg
      361 ccggcacccg ggaagtgcgg cgcttccgtc tgagtggcca tggtcacagc atggttatca
      421 ctgacctgcc cggggtgggc gagagccggg acagggatgc agagtatgaa gccctgtacc
      481 gtgacattct gcctgaactg gacttggtac tgtggctgat taaagccgat gaccgtgccc
      541 tgtctgtgga tgagtatttc tggcgacaca tactgcaccg gggacatcag caggtgctgt
      601 ttgtggtgac gcaggccgac aaaacagagc cctgccatga atgggatatg gcaggtattc
      661 agccttctcc cgcacaggaa cagaacattc gcgaaaaaac agatgcggta ttccggctgt
      721 tccggcccgt acatccggtt gttgccgtat cggcccgcac cggctgggaa ctggatacgc
      781 tggtcagtgc gctcatgaca gcgcttcccg accatgccgc cagtcccctg atgacccgcc
      841 tgcaggacga gctgtgcacg gaatctgtct gggggcaggc ccgtgaacag tttaccggtg
      901 cggtggaccg gatatttgac acggtggaga gcgtctgtgt tgcctctgtt gcacgcacag
      961 ttctgcgtgc cgtccgtgac acggtggtct ctgttgcccg cgcggtatgg aactggattt
     1021 tcttctgaac agtctgccag gggaaatgtc tgattacatt gcgttaacca actgaaaaca
     1081 tgatacaaac acctcatgcc cctcctgttt ctcttgcgtg actgctctac tgttaataga
     1141 ataaaacgat cgataaaaca ggtatcgatc gtttatatcg atcgtttata gtggttaatg
     1201 gcctgcgaaa aggcagctga atctcttcat catgcagaac ggaattgcac acaacagact
     1261 gatacttctc tgtctgatga agggggagag cgtttacgcc cgggacgtat cgctgtgccc
     1321 gataactctg ttttgtacct gccggtatcc acttttgtgg gtaccggctt ttttattcac
     1381 cctctgtaag gaaaagctga tgaaacgaca tctgaacacc agctacaggc tggtatggaa
     1441 tcacattacg ggcaccctgg tggtggcctc cgaactggcc cgctcacggg gaaaacgcgc
     1501 cggtgtggcg gttgcgctgt ctcttgctgc tgtcacatca gtcccggcac tggctgctga
     1561 caaggttgta caggcgggag aaaccgtgaa cgatggaaca ctgacaaatc atgacaacca
     1621 gattgtcttc ggtacggcca acggaatgac catcagtacc gggctggaac tggggccgga
     1681 cagtgaagaa aacaccggtg ggcaatggat acagaatggc gggatagccg gaaacaccac
     1741 tgtcaccaca aatggtcgtc aggtcgtgct ggagggggga acagccagtg atacggttat
     1801 tcgtgacggc gggggacaga gcctgaacgg actggcggtg aacaccacac tgaataacag
     1861 aggcgagcag tgggtgcatg agggcggggt tgccaccggt acaattatca accgcgacgg
     1921 ttaccagagc gttaaaagtg gcgggctggc aacaggaacc atcatcaaca ccggcgcaga
     1981 aggcggccct gattctgaca actcgtatac gggtcagaag gtccagggaa cagcagaatc
     2041 caccaccatc aacaaaaatg gacggcagat tatcttattt tccgggctag cccgtgacac
     2101 tctcatttac gcaggtggtg accagtcggt acacggaagg gccctgaata ccacactgaa
     2161 tggcggttac caatatgtgc acagggacgg acttgcgctg aacacggtaa ttaacgaggg
     2221 gggctggcag gttgttaagg caggtggcgc tgccggtaac accaccataa atcagaacgg
     2281 tgaactgagg gtacatgccg gcggggaagc cactgcagtc acccagaaca cgggcggtgc
     2341 actggttacc agtactgctg caactgtcat cggcacaaac cgtctgggga atttcacggt
     2401 ggaaaacggt aaggctgacg gtgttgttct ggaatccggc ggtcgtctgg atgtactgga
     2461 gagccattca gcacagaata ccctagtgga tgacggcggt accctggcag tgtctgccgg
     2521 cggtaaggcg acaagtgtca ccataacatc cggtggtgcc ctgattgcag acagtggtgc
     2581 cactgttgag gggaccaatg ccagcggtaa gttcagtatt gatggcacat ccggtcaggc
     2641 cagcggcctg ctgctggaaa atggcggcag ctttacggtt aatgccgggg gacaggctgg
     2701 caacaccact gtcggacatc gtggaacact gacgctggct gccgggggaa gtctgagtgg
     2761 cagaacacag ctcagtaaag gcgccagtat ggtactgaat ggtgatgtgg tcagtaccgg
     2821 cgatattgtt aacgcagggg agattcgctt tgataatcag acgacaccga atgccgcgct
     2881 gagccgtgct gttgcaaaaa gtaactcccc ggtaacgttc cataaactga ccaccacgaa
     2941 cctcaccggc cagggcggca ccatcaatat gcgtgttcgc cttgatggca gcaatgcctc
     3001 tgaccagctg gtgattaatg gtggtcaggc aaccggcaaa acctggcttg cgtttacaaa
     3061 tgtcggaaac agcaacctcg gggtggcaac caccggacag ggtatccggg ttgtggatgc
     3121 acagaatggc gccaccacag aagaaggtgc gtttgccctg agtcgcccgc ttcaggccgg
     3181 cgcctttaac tacaccctga accgtgacag cgatgaagac tggtacctgc gcagtgaaaa
     3241 tgcttatcgt gctgaagtcc ccctgtatac atccatgttg acacaggcaa tggactatga
     3301 ccggattctg gcaggctccc gcagccatca gaccggtgta aacggtgaaa ataacagcgt
     3361 ccgtctcagc attcagggcg gtcatctcgg tcacgataac aacggcggta ttgcccgtgg
     3421 agccacgccg gaaagcagcg gcagctatgg cttcgtccgt ctggagggtg acctgctcag
     3481 aacagaggtt gccggtatgt ctctgacgac aggggtgtat ggtgctgcag gccattcttc
     3541 cgttgatgtt aaggatgatg acggttcccg cgccggcacg gtccgggatg atgccggcag
     3601 tctgggcgga tacctgaatc tggtacacac atcctccggc ctgtgggctg acattgtggc
     3661 ccagggaacc cgtcacagca tgaaagcgtc atcggacaat aacgacttcc gcgcccgggg
     3721 ctggggctgg ctgggctcac tggaaaccgg tctgcccttc agtatcactg acaatctgat
     3781 gctggagcca caactgcagt acacctggca gggactctcc ctggatgacg gccaggataa
     3841 cgccggttat gtgaagttcg ggcatggcag tgcacaacat gtgcgtgccg gtttccgtct
     3901 gggcagccac aacgatatga cctttggtga aggcacctca tcccgtgaca ccctgcgcga
     3961 cagtgcaaaa cacagtgtga gtgaactgcc ggtgaactgg tgggtacagc cttctgttat
     4021 ccgcaccttc agctcccggg gtgacatgag catggggaca gccgcagccg gcagtaacat
     4081 gacgttctca ccgtcccgga atggcacgtc actggacctg caggccggac tggaagcccg
     4141 tatccgggaa aatatcaccc tgggcgttca ggccggttat gcccacagcg tcagcggcag
     4201 cagcgctgaa ggctataacg gtcaggctac gctgaatatg actttctgat tatacggtat
     4261 cccggaaata cgatttcagg aaaattcatg cagtaatggg aaaggacgct cattgctgtt
     4321 caccgcagtt ctgaattatt gaatttataa aggtgaatga tgcacgaaaa gaacatcgcc
     4381 ctgctttgtg atgaagccga ccggcttttg caactgaata tcaatctgct ccggcaaatg
     4441 gttgatgagc cggatgtgtt atctgacagt aagaacgaaa acgggctgct ttttgataaa
     4501 cggaaagcgc tgaaaagaat tgaggagctg gagggcgaac aaatcaaaac cgcccgcagg
     4561 gagatggtgc tggctgtggt cggcacgatg aaggcgggca aatcaaccac catcaacgcc
     4621 attgtggggc aggaaattct gcccaaccgt aaccgcccca tgacctcggt accgacattg
     4681 atccgccacg ttcccggaaa aacggaaccg gtcctccatc tggaacatat tcagcccgtc
     4741 cgcaatttat taatcacact gcaggaaaaa ctcgccaccc cggcagggca gcaagtcgca
     4801 cagtccctgc agcaaaccgg ggatacccgc gaactactgg atattctggc ggatgatgtc
     4861 tggcttaaaa atgaatacca cggggaagac gaaatcttta ccggactggc atcgttaaat
     4921 gacctggttc gtcttgctgc ggcaatgggg actgaatttc cttttgacga atacgcagaa
     4981 gtgcagaaac taccggtgat cgacgtggcg ttcagccacc tggtgggaat ggatgcatgt
     5041 cagggaacac tcacactgct ggataccccc ggccctaatg aagccggaca accacagatg
     5101 gaaatgatga tgcgggatca actacagaaa gcctctgcgg tgctggcggt gatggattac
     5161 acccagatga actcgaaagc cgatgaggag gttcgtaaag agcttaatgc cattgctgac
     5221 gtctcagccg gtcgtctgtt tgtgctggtc aataaattcg atgaaaaaga tcgcaatggt
     5281 gatggtgcgg atgccgtacg ccagaaggtg ccagcaatgc tgaacagcga tgtgctgccc
     5341 gcctcccgcg tttatcccgg ctcatcacgt caggcgtacc tggctaaccg tgcgcttcat
     5401 gagttacgaa aaaacggtgc gcttccggtt gatgaagcct gggtcgatga ttttatcagg
     5461 gaagctttcg gacctatggt ggaagaggac gactggaaag acagcactaa ggtaaataaa
     5521 aaggcagaaa aactctggaa tatatctctg atcgatcaac tgataacgga agtcatacag
     5581 agctcccatt ccagagcggc ggcactggcg gttgactctg ccgccgcaaa actgatgcag
     5641 aatgcagaaa atgtcagcga atatctgtca ttacgtcatc aggggctgca gcaaagcatt
     5701 cagtcactac aggcgcatat taccagcctg ctggcggata tccaggagat tgaggaatgt
     5761 cagaatcagg tgaccggtga tgtcagaatg gccatggagg acatcaatac caaaaccggg
     5821 gaattactga cgaaggtctg tgcctcactg gaggaggagc tgaacgacta tttcagaagc
     5881 ggtaaacgca aagaacagca aatgctggag gaagaaaatt cagcgcagcc ccgggagaga
     5941 aacgcattcg ctttctttca cgatattttc ggaaccggaa atcagcatga caggatgcgg
     6001 gactttgatc cggacagccc cgaaataaaa ttcagtgatc gcagggcagc ccttgaactg
     6061 atgacgcaaa tcgaatcgac cgtgaccagc ctgcaccgtg aggctgaagc acagttccgg
     6121 cctgagctgg agaaaatc
//
LOCUS       DBFIFM010000238         6118 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.238, whole genome shotgun sequence.
ACCESSION   DBFIFM010000238 DBFIFM010000000
VERSION     DBFIFM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.238"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(1..514)
                     /locus_tag="N7C74_004219"
                     /pseudo
     CDS             complement(1..514)
                     /locus_tag="N7C74_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498860.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(519..683)
                     /locus_tag="N7C74_004220"
     CDS             complement(519..683)
                     /locus_tag="N7C74_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433970.1"
                     /translation="MSGWCRPEIMILTMKVKCIGSLIGRSEAAVRMKAQVKGISLILR
                     GDFHQSTKYP"
     gene            complement(680..1360)
                     /locus_tag="N7C74_004221"
     CDS             complement(680..1360)
                     /locus_tag="N7C74_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU2433971.1"
                     /translation="MAARLDISREEVLNELWELKKAGFVDKSVYTWRVADNNVQQEQP
                     APEETTTATEAKISECDLTATIEQRGPQTADELATLFGTTSRKVASTLAMAISKGRLI
                     RVNQNGKIRYCIPGDNLPAEPKVESVAETDGKAFPQPAGVALPVQKDATQEDIKTETV
                     ADIVQSLPSFTATREDDLILPSLHMANRELRRAKNHVQKWERVCAALRELNKHRDMVA
                     GICRKSGQ"
     gene            complement(1448..1909)
                     /locus_tag="N7C74_004222"
     CDS             complement(1448..1909)
                     /locus_tag="N7C74_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU2433972.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISKSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWSQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPSGPTPIELLKQEYERRKAAGFV"
     gene            complement(1902..2942)
                     /locus_tag="N7C74_004223"
     CDS             complement(1902..2942)
                     /locus_tag="N7C74_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HGU2433973.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGAVNKNSTENGYVSAQNRPKNRTLSCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPPVFLYGLSEPIGKFPMSDSWYPSRDFRRRAA
                     LWGMALPETEFTPAELAAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            complement(2914..3465)
                     /locus_tag="N7C74_004224"
     CDS             complement(2914..3465)
                     /locus_tag="N7C74_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU2433974.1"
                     /translation="MKITTEQVCEALDTWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKRDKFTALIPAIMEAIRISDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPVVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQMSARGAYEYRCTTMG"
     gene            complement(3449..3676)
                     /locus_tag="N7C74_004225"
     CDS             complement(3449..3676)
                     /locus_tag="N7C74_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGU2433975.1"
                     /translation="MLKIDAIAFFGSKTKLANVAGVRLASVAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHH"
     gene            3753..4160
                     /locus_tag="N7C74_004226"
     CDS             3753..4160
                     /locus_tag="N7C74_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2433976.1"
                     /translation="MKNETFGARLLYRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPVNLTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            4424..4723
                     /locus_tag="N7C74_004227"
     CDS             4424..4723
                     /locus_tag="N7C74_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433977.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDLATTFWARKLDCIHNQFPLE"
     gene            4796..5014
                     /gene="ydfC"
                     /locus_tag="N7C74_004228"
     CDS             4796..5014
                     /gene="ydfC"
                     /locus_tag="N7C74_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HGU2433978.1"
                     /translation="MQKRDPVIIAPDYTDDELYEWMHQKIKAVQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            5037..5444
                     /locus_tag="N7C74_004229"
     CDS             5037..5444
                     /locus_tag="N7C74_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433979.1"
                     /translation="MEFKDLPTPLQEMASNIVRSQLATLDLSTAEKETIDNMVRNVRN
                     AFSGLYGSDNQKQESDVNKRVISVCVNGHVLSSIKTETATVFDCLCIVQSLVDALFRS
                     VNLENDANLRGRIIAHPYAHTLGSVDIKDPTNL"
     gene            complement(5422..5655)
                     /locus_tag="N7C74_004230"
     CDS             complement(5422..5655)
                     /locus_tag="N7C74_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2433980.1"
                     /translation="MLKSTLIAKCLYQNRMVSSISIGESAVKSIFEEYFPGHDFNKWN
                     TKLPPAVSTRILKATERASTIRVNYFIKDLWDL"
BASE COUNT         1511 a         1593 c         1360 g         1654 t
ORIGIN      
        1 tgtaatcaac atcctgtttg gaatagccag cctttaatgg ataactattt ctgacaatgc
       61 aatgagtata atcaagtcca tcttccactg agaattagag gcggcatgct ttttccggct
      121 cttgccggat atccgtaatt gtccataatc tgcagattta taccttctgc atgacctgtc
      181 agcgaaaatt tgtccggtgt ttctacggga atgacatcaa aagttacacg cactcgcgtt
      241 accgtgtaga cctactttcc tgcacttgca agatcacagt ggtgtaaccg taacaggaat
      301 ttattctctg gaccggcagt aaatccctga gtggcgtggt tcccatatca atttcccgcc
      361 aggcagcctc cattgccagc gtacaggctg gagccatgac ctgcccttta aatctggccc
      421 gaccatccca ccggacgtgt tcttctcccc tgaacttagg tacagtcatc tccagtggca
      481 caaaagtgtc agcgccataa tttttgaccg ttatcgcgct acggatattt tgttgactgg
      541 tgaaaatcac cccgcagaat caggcttatt cccttaacct gggctttcat cctgaccgcc
      601 gcctcactac gaccaatcag actgccgatg cattttacct tcattgttag tatcatgatt
      661 tcaggcctgc accatccgct cattgcccgg acttccgaca aatcccggca accatatccc
      721 ggtgcttgtt cagctcccgc agcgcggcgc agactcgctc ccacttctgg acatgattct
      781 tcgccctacg cagttcgcga tttgccatat gcagcgatgg caaaatcaaa tcatcttctc
      841 gcgttgcagt aaacgatggc agcgactgca caatgtccgc cacagtttct gttttaatat
      901 cttcctgtgt tgcatctttc tgtaccggta acgcaacacc ggctggctga ggaaaggctt
      961 taccatcggt ttccgctacc gattcaactt tcggctctgc tggtaaatta tcacctggta
     1021 tgcagtaacg aattttaccg ttctggttta cgcgaatcag acgacctttg ctgattgcca
     1081 ttgccagcgt tgaagcaact ttgcgggatg ttgtaccgaa cagcgtagcc agttcatccg
     1141 ccgtttgtgg tccgcgttgt tcaatcgtcg cggttaaatc gcactctgag attttcgctt
     1201 ctgttgccgt ggtggtttct tctggcgctg gctgttcctg ctgaacgttg ttatcagcca
     1261 cacgccaggt gtatacgctt ttatcaacga agccagcctt tttcagttcc cacagctcgt
     1321 tcagtacttc ttcacgactg atatcaagtc gcgcagccag ctataccgac gtggcttttc
     1381 ccatcgcttt cagtgcgtca aaaacagtct ccataaattt cctcccggta aaaaatcact
     1441 tctcaactca aacaaaacca gccgctttcc ggcgttcata ttcctgtttc agcaactcaa
     1501 ttggcgttgg ccccgacggg cgtttggggg ccgccagttg tcgccggact ggcggaacgc
     1561 tcaggccgtt actaacatgc tttgcccatt tcgtcagttg ccgttctgca agccgtttta
     1621 attccccttc ggtcatctgg cgctcaatcc cctttgaacg catctcgagg caaatgtgat
     1681 acagcacagg ctgagaccac gggtacttat cacttccgtc gtatcgccag gactcattgc
     1741 gccagcggcg gtactcctcc atcacagcat ccaccgtcag accgaatgga ttggctccgc
     1801 ttttcgaaat cagtgccaca aactcagcca ggtccggagg ccatgtttca cccgcccggc
     1861 agcggtccat gcactggcgg cagacctgcc ggatttgctg ctcagtcatc gcgccaatct
     1921 gtgcaatcca gagcttcgaa ggtgcggccc cgttcttctg gatccagcgg ttcgaataaa
     1981 ccacccccat gagttcccac agcttccagg ccgtttccgt cgctgataaa tccgttttca
     2041 cgttcccact gctcacgtgc tgcccgaatt tcctgaactg cccgtgatgc ggtgccacct
     2101 ggtgctgctg catggtttac ccctttgctg actggtttaa cctgcgccct gacgtgattt
     2161 acgtgacggg cgaatttctg ctcccactga atctgcgtaa acactttccc ctccgctgcc
     2221 cagtagtccc ggaaggcggc aagttcagca ggtgtaaatt ctgtctccgg caaagccatc
     2281 ccccacaacg cagcccgtcg tcgaaaatcc cgtgacggat accagctatc gctcatcggg
     2341 aattttccga tgggttcgct caggccatac aggaatacag ggggggctgc ctgtaacgac
     2401 aaaacttcct gctcactggt cggagcactc tcgcgtgcgt tatgtgtggg gtttagatct
     2461 ttgggttcct ttgggttccg tgatccgttt ttgggtgtct ttgatggaaa atttgggtgt
     2521 ctttggttat tttccatgca gctaagagtt ctgtttttgg gtctgttttg tgctgaaaca
     2581 taaccatttt cggtactgtt tttattaaca gcaccaattt tacccacctt taaagactcc
     2641 cgtttttggg tgtattcagg ctcggcaaca ctttcttcta caccgataag tcggtacacc
     2701 acaatttgct ttgttctgcc ttttctctca ccggtatcaa caattaaccc aatctccatc
     2761 aggtgtcgta agctgtcctg cacagtcttt ttgttcagtt ccgttacttc tgccagtgca
     2821 gatacagacg ggtatgcaca caaatcggca ccgcacatat cagcaagcca ggtcaatact
     2881 gacttactgg atgaactgcc ggttttcacc tttttagccc atcgtagtgc atcgatactc
     2941 atacgccccc ctggcagaca tttgtttatc tgcaaagtaa tattggtatt gctgacgata
     3001 cgcgtgcttg aaagcaatag ctttttctat aagctcgtca gtctcacgtt ccacaacaac
     3061 tggatccgca aaaagcagcc cggactccac cacatcgcca tattctttgt ttaacccggc
     3121 gatcatgtac gtaatgcttt ttccgtcagt aatttcacaa tacaatctga aatcgctgat
     3181 ccggatagcc tccataattg ccggaatcag cgccgtgaat ttgtcacgct tatctctggt
     3241 gtcgatagct ttccagcgtt cgaatatctt cacccggtta acgcccagcg cccgttgatc
     3301 aacctcgcca tcattaaacg tgacgcgttg aacatcgatg ttcgggcgtt ctttcagagc
     3361 ccagaatgct tccgtgatta atatcgtcgc ctgctcctgt gtcattcctg gtcggcatac
     3421 ccaggtatcc agagcctcac aaacctgttc agtggtgatt ttcattgttc aaccgccccg
     3481 cccgctttgc cttacgatat tcgtcataaa ctttggggtc gtactgaagt tccccgccgg
     3541 atgcctcttg caggcgcatc gcgcgacctt caggaaccag ttccccccat gcagcaacgc
     3601 ttgccagcct aactcctgcg acattggcaa gctttgtttt gctgccaaaa aacgctatag
     3661 catcaatttt caacatatcg aaacccttag attttcctaa ggaaactaga tcgtagagaa
     3721 acctaagtca agaaaaatta gaattaccta atatgaaaaa cgaaaccttc ggtgctcgcc
     3781 tcttatacag gcgaaaaaaa ttaaaactgt ctcaggccgc attaggtaag ctggtcaaag
     3841 tggctcacgt aacaatttct caatgggaaa gagatgaaac acagccagcg gggaagagat
     3901 tattcgcact gagccaggcg cttcagtgct cgccgacttg gcttcttttt ggagatgaag
     3961 ataagcaacc aggcgaaccg atcccagata atcagccagt caatctgaca gaagatcaaa
     4021 aagagttgct tcaactgttc gacgcactgc ctgagtcaga gcaaaaggct ctgttgtcag
     4081 agatgcgtgc tcgagttgag aatttcaaca aactttttga agaactactc aaagctcgca
     4141 aaagaagcgc aaataaataa cccccctttt tttcgcaact ctctgtaata aaaagcacaa
     4201 actttcaaat acttgtgttt tttacatcaa gaagcttagg tttttctaca caaaaagctt
     4261 gaccataact cttaggctat tctaaattct actcatcaag acaccgcacg gtgttctcag
     4321 caaacagttc cgctacccgg cgttaagggg aagcagagga tttctcagtg ggcgagtcaa
     4381 acatcagaat ggaaggcgtc cagggatcag caaagaaaca gcgatggcgc tttatattga
     4441 tatcagcgcc attgccggac aggtaagagt tatcagagcg gtaactaagc ggtatgcgcc
     4501 tttacttcag aaagtctctg gtgagtgcac cgaagatatt gtcaacgatt tcgtcatcga
     4561 actgcgagga ctcatcttca gttacaaggt gaccacaatt tttgcagatg gctcccgcga
     4621 aactgtcaga gccctgcggc ttaaaggatg tgtcaaagac ttagccacca cattctgggc
     4681 aagaaaactt gattgtattc ataaccaatt tcctctcgag taacagaccc ctcagaggat
     4741 accacctcgc ctgacgtggt taaaaagcag gcaacgctaa ccacaaggag ccgacatgca
     4801 gaaacgagac cccgtcatca tcgcgccaga ctataccgat gatgaacttt atgagtggat
     4861 gcaccagaaa attaaggcgg tgcaggacct gaaatgggcc aatgaagcca gggctaagca
     4921 ggctgaaaat ctgtccgctc tggagcagga tatcaccaat ctggaaaaag cagcggcatt
     4981 aagcattgcc agaatgatta catacccacg ttaatagcta accaacgagg ctaataatgg
     5041 aatttaaaga tttaccaacg ccattacagg aaatggcatc gaatatagtt cgttcacaac
     5101 tggctactct tgaccttagt accgcagaaa aagaaaccat cgataatatg gttcgtaatg
     5161 tgcgcaatgc tttttctggg ctatatggtt ctgataatca aaagcaggaa agcgatgtta
     5221 ataaacgggt aatttctgtt tgcgtgaatg gccatgttct ttcatcaatc aaaacagaaa
     5281 cggcgacagt cttcgattgc ctttgcattg tacagagcct tgttgatgcc ctgtttcgtt
     5341 cagtgaattt agaaaatgat gcaaatctgc gagggcgcat aatagcacat ccatatgcac
     5401 atactttagg ctctgtggat atcaaagatc ccacaaatct ttaatgaaat agttaacgcg
     5461 aattgtactt gctctttcgg ttgctttcag aatacgcgtt gaaactgctg gcggtaattt
     5521 ggtattccat ttattaaaat catgcccggg aaagtactct tcgaaaatac ttttaactgc
     5581 agactcgcct attgaaatgc tgcttaccat gcgattttga taaaggcatt tagcaataag
     5641 agttgatttt aacattcacc ctcctgaggg ttggtaatta aggagttctc cacgggtgaa
     5701 gtggagtgcg tgcgccggac acgggtgaac atccggcact gacagtttac tgaaaggata
     5761 tttctctgaa aagtcagagc ataacgcgaa agcgcacggc gaggttgctg gttcatagat
     5821 agcctgtcgt taaattttcg tcgaccgtgc gcttccggtt gtggcactcc gcgaaatggc
     5881 gcagcggtaa gtatggcggg gttattcctt ccccgttgag gacaccgggt tgtcaggttg
     5941 accatacgct taagtgacaa ccccgctgca acgccctctg ttatcaattt tctggtgacg
     6001 tttggcggca tctgtttgcc catgaactga tgtccgccct ttttaaagtg aattttgtga
     6061 tgcagtgaat gcggctaagc gcacgcggaa cagttaaagc taaaaacagt gttatggg
//
LOCUS       DBFIFM010000239         6072 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.239, whole genome shotgun sequence.
ACCESSION   DBFIFM010000239 DBFIFM010000000
VERSION     DBFIFM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.239"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..253
                     /locus_tag="N7C74_004231"
     CDS             <1..253
                     /locus_tag="N7C74_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGU2433981.1"
                     /translation="SGAVIRGVFDDPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRG
                     DTLTIGEENFWIDRISTDDGGSCHLWLGRGVPPAVNRRR"
     gene            265..843
                     /locus_tag="N7C74_004232"
     CDS             265..843
                     /locus_tag="N7C74_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2433982.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGASAMAINRVASSAISQSASQV
                     ARETRVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            840..1235
                     /locus_tag="N7C74_004233"
     CDS             840..1235
                     /locus_tag="N7C74_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGU2433983.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            1243..1983
                     /locus_tag="N7C74_004234"
     CDS             1243..1983
                     /locus_tag="N7C74_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2433984.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYNGNGDPYANPLSDNDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWAATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            1999..2421
                     /locus_tag="N7C74_004235"
     CDS             1999..2421
                     /locus_tag="N7C74_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU2433985.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPAEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            2403..2837
                     /locus_tag="N7C74_004236"
     CDS             2403..2837
                     /locus_tag="N7C74_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU2433986.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            2830..5379
                     /locus_tag="N7C74_004237"
     CDS             2830..5379
                     /locus_tag="N7C74_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU2433987.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSMSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGEEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRMLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAVLGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLRSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGGASASGGTAIQAAAAKFHFAAGGFTGTGGK
                     YEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQASGTFE
                     QNNHVVINNDGTNGQIGPQALKAVYDVARKAAMDVVTGQMRDGGLFSGGGR"
     gene            5376..5705
                     /locus_tag="N7C74_004238"
     CDS             5376..5705
                     /locus_tag="N7C74_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2433988.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            5705..>6072
                     /locus_tag="N7C74_004239"
     CDS             5705..>6072
                     /locus_tag="N7C74_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU2433989.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGN"
BASE COUNT         1410 a         1487 c         1986 g         1189 t
ORIGIN      
        1 gtccggcgca gtaatacgtg gtgtttttga tgaccctgaa aatatcagct atgccggaca
       61 gggcgtgcgt gttgaaggct ccagcccgtc cctgtttgtc cggactgatg atgtgcggca
      121 actgcggcgt ggagacacgc tgaccatcgg tgaggaaaac ttctggatag accggatttc
      181 gacggatgat ggcggaagct gtcatctctg gcttgggcgg ggcgtaccgc ctgccgttaa
      241 ccgtcgtcgc tgaagggggg atgtatggcc ataaaaggtc ttgagcaggc cgttgaaaac
      301 ctcagtcgta tcagcaaaac ggcggtgcct ggtgcgtcag caatggccat taaccgcgtt
      361 gcttcatccg cgatatcgca gtcggcgtca caggttgccc gtgagacaag agtacgccgg
      421 aaactggtaa aggaaagggc caggctgaaa agggccacgg ttaaaaatcc gcaggccaga
      481 atcaaggtta accgggggga tttgcccgta atcaagctgg gtaacgcgcg ggttgtcctg
      541 tcccgacgca ggcgtcgtaa aaaggggcag cgttcatccc tgaaaggtgg cggcagcgtg
      601 cttgtggtgg gaaaccgtcg tattcccggc gcgtttattc agcaactgaa aaatggccgg
      661 tggcatgtca tgcagcgtgt ggccgggaaa aaccgttacc ccattgatgt ggtgaaaatc
      721 ccgatggcgg tgccgctgac cacggcgttt aaacagaata ttgagcggat acggcgtgaa
      781 cgtcttccga aagagctggg ctatgcgctg cagcatcaac tgagaatggt aataaagcga
      841 tgaaacatac tgaactccgt gcagccgtac tggatgcact ggagaagcat gacaccgggg
      901 cgacgttttt tgatggtcgc cccgctgttt ttgatgaggc ggattttccg gcagttgccg
      961 tttatctcac cggcgctgaa tacacgggcg aagagctgga cagcgatacc tggcaggcgg
     1021 agctgcatat cgaagttttc ctgcctgctc aggtgccgga ttcagagctg gatgcgtgga
     1081 tggagtcccg gatttatccg gtgatgagcg atatcccggc actatcagat ttgatcacca
     1141 gtatggtggc cagtggctat gactaccggc gcgacgatga tgcgggcctg tggagttcag
     1201 ccgatctgac ttatgtcatt acctatgaaa tgtgaggacg atatgccaac accaaatcct
     1261 ctggcaccgg tgaaaggggc cgggaccaca ctgtgggttt ataacgggaa cggcgaccca
     1321 tatgcaaacc cgctttcaga caatgactgg tcgcgtctgg caaaggttaa agacctgacg
     1381 cccggcgaac tgaccgctga gtcctatgac gacagttatc tcgatgatga agatgcggac
     1441 tgggccgcga ccggacaggg gcagaaatcc gctggagata ccagcttcac gctggcgtgg
     1501 atgcccggag agcaggggca gcaggcgctg ctggcgtggt ttaatgaagg tgatacccgt
     1561 gcctataaaa tccgcttccc gaacggcacg gtcgatgtgt tccgcggctg ggtcagcagt
     1621 atcggtaagg cggtgacggc gaaggaagtg atcacccgca cggtgaaagt caccaacgtg
     1681 ggacgtccgt cgatggcaga agatcgcagc acggtaacag cggcaaccgg catgaccgtg
     1741 acgcctgcca gcacctcggt ggtgaaaggg cagagcacga cgctgaccgt ggcattccag
     1801 ccggaaggcg caaccgacaa gagcttccgt gcggtgtctg cggataaaac aaaagccacc
     1861 gtgtcggtca gtggtatgac catcaccgtg aaaggtgttg ctgcaggcaa ggtcaacatt
     1921 ccggtcgtat ccggtaatgg tgagtttgct gcggttgcag aaatcaacgt caccgccagt
     1981 taatccggag aatcagcgat gttcctgaaa accgaatcat ttgaacataa cggcgtgacc
     2041 gtcacgcttt ctgaactgtc agccctgcag cgtattgagc atctcgccct gatgaaacgg
     2101 caggcagaac aggcggagtc agacagcaac cggaagttta ctgtggaaga cgccatcaga
     2161 accggcgctt ttgtggtggc gatgtccctg tggcataacc atccgaagaa gacgcagatg
     2221 ccgtccatga atgaagccgt taaacagatt gagcaggaag tgcttaccac ctggcccgcg
     2281 gaggcaattt ctcatgctga aaacgtggtg taccggctgt ccggtatgta tgagtttgtg
     2341 gtgaatgatg cccctgaaca ggcagaggac gccgggcctg cagagcctgt ttctgcggga
     2401 aagtgttcga cggtgagctg agttttgccc tgaaactggc gcgcgagatg gggcgacccg
     2461 actggcgtgc catgcttgcc gggatgtcat ccacggagta tgccgactgg caccgctttt
     2521 acagtaccca ttattttcat gatgttctgc tggatatgca cttttccggg ctgacgtaca
     2581 ccgtgctcag cctgtttttc agcgatccgg atatgcatcc gctggatttc agtctgctga
     2641 accggcgtga ggctgacgaa gagcctgaag atgatgtgct gatgcagaaa gcggcagggc
     2701 ttgctggagg cgttcgtttt ggcccggacg ggaatgaagt tatccccgct tccccggatg
     2761 tggcggacat gacggaggat gacgtaatgc tgatgacagt atcagaaggg atcgcaggag
     2821 gagtccggta tggctgaacc ggtaggcgat ctggtcgttg atttaagtct ggatgcggcc
     2881 agatttgacg agcagatggc cagagtcagg cgtcattttt ccggtacgga aagtgatgcg
     2941 aaaaaaacag cggcagtcgt tgaacagtcg atgagccggc aggcgctggc tgcacagaaa
     3001 gcgggaattt ccgtcgggca gtataaagcc gccatgcgta tgctgcctgc gcagttcact
     3061 gacgtggcca cgcagcttgc aggtgggcaa agtccgtggc tgatcctgct gcaacagggg
     3121 gggcaggtga aggactcctt cggcgggatg atccccatgt tccgggggct tgccggtgcg
     3181 atcaccctgc cgatggtggg ggccacctcg ctggcggtgg cgaccggtgc gctggcgtat
     3241 gcctggtatc agggcaactc aaccctgtcc gatttcaaca aaacgctggt cctttccggc
     3301 aatcaggccg ggctgacggc agatcgtatg ctggtcctgt ccagagccgg gcaggcggca
     3361 gggctgacgt ttaaccagac cagcgagtca ctcagcgcac tggttaaggc gggggtaagc
     3421 ggtgaggctc agattgcgtc catcagccag agtgtggcgc gtttctcctc tgcatcaggc
     3481 gtggaggtgg acaaggtcgc tgaagccttc gggaagctga ccactgaccc gacgtcgggg
     3541 ctgacagcga tggcgcgcca gttccataac gtgacggcgg agcagattgc gtatgttgct
     3601 cagttgcagc gttccggcga agaagccggg gcattgcagg cggcgaacga ggccgcgacg
     3661 aaagggtttg atgaccagac ccgccgcctg aaagagaaca tgggcacgct ggaaacctgg
     3721 gcagacagga cagcacgggc attcaaatcc atgtgggatg cggtgctgga tattggtcgt
     3781 cctgataccg cgcaggagat gctgattaag gcagaggccg cgtttaagaa agcggacgat
     3841 atctggaatc tgcgcaagga tgattatttt gttaacgatg aagcgcgggc gcgttactgg
     3901 gatgatcgtg aaaaggcccg tcttgcgctt gaagccgccc gaaagaaggc tgagcagcag
     3961 agtcaacagg acaaaaatgc gcagcaacag agcgataccg aagcgtcacg gctgaaatat
     4021 accgaagagg cgcagaaggc ttacgaacgc ctgcagacgc cgctggagaa atataccgcc
     4081 cgtcaggaag aactgaataa ggcactgaaa gacgggaaaa tcctgcaggc agattacaac
     4141 acgctgatgg cggcggcgaa aaaggactat gaagcgacgc tgaaaaagcc gaaacagtcc
     4201 ggcgtgaagg tgtctgcggg cgatcgtcag gaagacagtg ctcatgctgc cctgctgacg
     4261 cttcaggcag aactccggat gctggagaag catgccggag cgaatgagaa aatcagccag
     4321 cagcgccggg atttgtggaa ggcagaaagt cagttcgcgg tactggagga ggcggcacaa
     4381 cgtcgccagc tgtccgcaca ggagaaatcc ctgctggcgc ataaagacga gacgctggag
     4441 tacaaacgcc agctggctgt acttggcgac aaggtcacct atcaggagca cctgaacgcg
     4501 ctggcacagc aggcggataa attcgcacag caacaacggg caaaacgggc cgccattgat
     4561 gcaaaaaacc gggggctgac tgaccggcag gcagcgcggg aagccacgga acagcgcttg
     4621 aaggaacagt atggcgataa tcctctggcg ctgaataacg tcatgtcaga gcagaaaaag
     4681 acctgggcag ctgaagacca gcttcgcggg agctggatgg caggcctcag gtcaggctgg
     4741 agtgagtggg aagagagcgc cacggacagt atgtcgcagg ttaaaagtgc tgccacgcag
     4801 acctttgatg gtattgcaca gaatatggcg gcgatgctga ccggcagtga acagaactgg
     4861 cgcagcttca cccgttccgt gctgtccatg atgacagaaa ttctgcttaa gcaggcaatg
     4921 gtggggattg tcggaagtat aggcagcgcc attggcggcg gcgcatcagc gtcaggcggt
     4981 acagccattc aggcagctgc ggcgaaattc cattttgcgg ccggagggtt tacgggaacc
     5041 ggcggcaaat atgagccagc ggggattgtt caccgtggtg aatttgtctt cacgaaggag
     5101 gcaaccagcc ggattggcgt aggaaatctt taccggctga tgcgcggcta tgccaccggc
     5161 ggttatgtcg gtacaccggg cagcatggcg gacagccggt cgcaggcgtc cgggacgttt
     5221 gagcagaata accatgtggt gattaacaac gacggcacga acggtcagat agggccacag
     5281 gcactgaagg cggtttatga cgtagcccgt aaggcggcaa tggatgttgt gaccgggcag
     5341 atgcgcgatg gtggtctgtt ctccggaggt ggacgatgaa aaccttccgc tggaaagtga
     5401 aacccggtat ggatgtggct tcggcccctt ccgtaagaaa ggtgcgcttt ggtgatggct
     5461 attcccagcg agcgcctgcc gggctgaatg ccgacctgaa aacgtacagc gtgacgcttt
     5521 ctgttccccg ttgggaggcc acggcgcttg agtcgtttct ggctgagcac gggggctgga
     5581 aggcctttct gtggacgccg ccttatgagt ggcggcagat aaaggtgacc tgcgcaaaat
     5641 ggtcgtcgcg ggtcagtatg ctgcgtgttg agttcagcgc agagtttgaa caggtggtga
     5701 actgatgcag gatatccggc aggaaacact gaatgaatgc acccgtgcgg agcagtcggc
     5761 cagcgtggtg ctctgggaaa tcgatctgac agaggtcggt ggagaacgtt attttttctg
     5821 taatgagcag aacgaaaaag gtgagccggt cacctggcag gggcgacagt atcagccgta
     5881 tcccattcag gggagtggtt ttgaactgaa tggcaaaggc accagtacgc gccccacgct
     5941 gacggtttct aacctgtacg gtatggtcac cgggatggcg gaagatctgc agagtctggt
     6001 cggcggaacg gtggtccggc gtaaggttta cgcccgtttt ctggatgcgg tgaacttcgt
     6061 caacggaaac ag
//
LOCUS       DBFIFM010000240         5995 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.240, whole genome shotgun sequence.
ACCESSION   DBFIFM010000240 DBFIFM010000000
VERSION     DBFIFM010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.240"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            94..1207
                     /gene="ompN"
                     /locus_tag="N7C74_004240"
                     /pseudo
     CDS             94..1207
                     /gene="ompN"
                     /locus_tag="N7C74_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            1348..1782
                     /gene="uspF"
                     /locus_tag="N7C74_004241"
     CDS             1348..1782
                     /gene="uspF"
                     /locus_tag="N7C74_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HGU2433990.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1958..2893
                     /gene="ttcA"
                     /locus_tag="N7C74_004242"
     CDS             1958..2893
                     /gene="ttcA"
                     /locus_tag="N7C74_004242"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HGU2433991.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(3022..4395)
                     /gene="dbpA"
                     /locus_tag="N7C74_004243"
     CDS             complement(3022..4395)
                     /gene="dbpA"
                     /locus_tag="N7C74_004243"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HGU2433992.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4428..4598)
                     /gene="ynaL"
                     /locus_tag="N7C74_004244"
     CDS             complement(4428..4598)
                     /gene="ynaL"
                     /locus_tag="N7C74_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HGU2433993.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRA"
     gene            complement(4873..5856)
                     /gene="zntB"
                     /locus_tag="N7C74_004245"
     CDS             complement(4873..5856)
                     /gene="zntB"
                     /locus_tag="N7C74_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HGU2433994.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(5870..5917)
                     /gene="ydaN"
                     /locus_tag="N7C74_004246"
     CDS             complement(5870..5917)
                     /gene="ydaN"
                     /locus_tag="N7C74_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HGU2433995.1"
                     /translation="MLRNRARRLDYKIMR"
BASE COUNT         1590 a         1509 c         1411 g         1485 t
ORIGIN      
        1 atatttatcg gctaactgaa cttctccttt aggatgtttt cactccccga aatggggata
       61 aaaggcaaat aaaataacta aaggatttat tcaatgaaaa gcaaagtact ggcactttta
      121 attcctgccc tgctcggcgc aggtgctgca catgcagccg aagtttataa taaagacggc
      181 aacaaattag atctgtatgg caaagttgat ggcctgcatt atttttctga taattcagcg
      241 aaagatggcg accagagcta tgcgcgtctg ggttttaaag gcgaaaccca aattaacgat
      301 caacttactg gctacggtca atgggaatac aatattcagg caaacaacac tgaatcttca
      361 aaaaaccagt catggacccg tctggcattt gccggactga aattttcaga ttacggttct
      421 ttcgattacg gacgtaatta tgggctggac cgatatgctg cctgaatttg gcggtgactc
      481 ttataccaat gcagacaact ttatgactgg tcgtgccaat ggcgtcgcga cttatcgtaa
      541 tactgatttc ttcggtctgg taaatggtct gaacttcgcg gtgcagtatc aaggtaacaa
      601 cgaaggtgcc agtaatggtc aggaaggcac caacaacgga cgtgatgttc gccatgaaaa
      661 cggtgacggc tggggtcttt ccacaacata tgatttaggc atgggcttta gcgctggtgc
      721 ggcatacacc tcttctgacc gcaccaatga ccaggttaac catactgcgg cgggtggtga
      781 taaagcagac gcgtggactg ctgggctaaa atacgatgct aacaatattt acctggcaac
      841 catgtattca gaaacgcgta atatgacccc gtttggcgac agcgattatg ctgttgcaaa
      901 caaaacccag aactttgaag tcactgcaca gtaccagttt gattttggtc tgcgtccggc
      961 agtctctttc ctgatgtcta aaggccgtga cctgcacgct gccggtggtg cagacaaccc
     1021 ggcaggtgtt gatgataaag atctggttaa atacgccgat gttggcgcga cttactattt
     1081 caataaaaac atgtccactt atgttgacta taaaatcaac ctgttggatg aagatgacag
     1141 tttctacgct gccaacggca tctctaccga tgatattgtc gctttaggtc tggtttatca
     1201 gttctaaatc ctcctgcccg ctgttatggc gggctttttc tgcttattct tcctcctttg
     1261 atctaaatta aaaatgtgaa ctccgtcatt acacaaaaag tgtcatctgg cgttacactt
     1321 tatgcggata ctaaaacagg aggttttatg aacagaacga ttcttgtccc tatcgatatt
     1381 tccgattcag aattaactca acgcgtgatt agccacgttg aggcagaggc aaagattgat
     1441 gatgcagagg ttcatttcct gacggtaata ccttcactgc cctactatgc ctctctgggt
     1501 ttagcgtatt ccgcagaatt accggcaatg gatgacctga aagcggaagc caaatcgcaa
     1561 ctggaagaga tcattaaaaa atttaaactg ccaaccgaca gagtgcatgt ccatgttgag
     1621 gaaggctcgc ccaaagaccg cattctggaa ttggcgaaga agatccccgc tcatatgatc
     1681 atcattgctt cccatcgacc ggatatcacc acttatctgc tcggttccaa cgccgcagct
     1741 gtagtgcgtc acgcagagtg ctccgtgctg gttgtgcgct gacactaacg cccgcacatt
     1801 gctgcgggct ttttgattca tttcgcaaat gtgctgacat tttcccctct aatccgtacc
     1861 atacacgcca cagtttttat atcagatttc ttatgctggg cgttccggca tgacgcatac
     1921 tcttctgatg ccatataacg aattgagtcg cttttaaatg tcgcaaaatc aagaaattag
     1981 caagaaagaa caatacaacc tgaacaaatt acaaaaacgc ctgcgtcgta acgtgggcga
     2041 agccattgct gacttcaata tgattgaaga aggcgatcgc atcatggttt gcctctccgg
     2101 gggtaaagac agctatacca tgctggagat tctgcgcaat ttgcagcaaa gcgcgccaat
     2161 caatttttcg ctggtggctg ttaacctcga tcaaaagcaa ccgggcttcc cggaacacgt
     2221 tctgcccgag tatcttgaaa agctgggcgt tgagtacaag attgttgaag agaacactta
     2281 cggtatcgtg aaagagaaga ttccagaggg caaaaccact tgctcactgt gttctcggct
     2341 acgtcgcggt atcctttatc gcaccgcaac ggaactgggg gcgacgaaga tcgcgttggg
     2401 ccaccatcgt gacgatatcc tgcaaacgtt gttcttaaat atgttctacg gcggcaagat
     2461 gaaaggtatg cctccgaaac tgatgagcga tgatggcaaa catatcgtta ttcgtccgct
     2521 ggcctactgc cgcgagaaag atattcagcg atttgccgat gcaaaagcgt tcccgattat
     2581 tccgtgcaac ctgtgcggtt cacagcctaa cctgcaacgt caggtgattg ctgacatgtt
     2641 gcgtgactgg gataaacgtt atccagggcg tatcgagacg atgttcagcg cgatgcagaa
     2701 tgtggtgccg tcgcatctgt gcgataccaa cctgttcgat ttcaaaggca tcacccacgg
     2761 ttctgaagtg gttaacgggg gtgatctggc gtttgatcgc gaagagatcc cactacaacc
     2821 ggcgggctgg cagccagaag aagatgaaaa tcagttggat gagttacggc tgaatgtggt
     2881 tgaagtgaaa taactcggat agcgcccgat gcgcaaacgt atcgggctac tctaatggag
     2941 gctgaataag acgcggtcag cgtcgcatcc gacaatcccg acgaagatgt ttacttttgc
     3001 atcaggcaat tcaacatttt attattttaa taaccgcacc cggcacgttt ttcctttaat
     3061 cttcccgccc tgtaactgtt tccatgcttt atgagcaaca gcctgacgga ccgcgacata
     3121 gacatgcgcc ggatgcacgg cgattttgcc aatatctgcg ccatcaagcc cgatatctcc
     3181 tgtcagtgca cctaatacat cacccgggcg cattttggct tttttcccgc catcgataca
     3241 caacgttgcc atttctgctt ccagcgtcac aatggaacta ttagctggtg gcgtttgcca
     3301 gttaagtttt atctgcaaca tgtcagaaat gatattggcc cgctgtgctt cttccggagc
     3361 acagaaactg atcgccagac cgctatttcc tgcacgagct gtacgaccga tgcgatgtac
     3421 atgaacttca gggtcccacg ccagctcaaa gttcaccacc agctcaagcg atttaatatc
     3481 cagaccacgc gcagcaacat cagtcgcgac cagtacacgg gcgctaccgt tagcaaaacg
     3541 taccagggtc tgatcacgat cgcgttgctc cagatcgccg tgtaatgaca atgcactttg
     3601 ccctacttca ttcagcgcgt cgcagacagc ctggcaatct tttttggtat tgcaaaacac
     3661 cacgcaagag gatggctgat gcaagcttaa taaccgttgc aacagaggaa ttttgccttt
     3721 gctggatgtc tcataaaatt gttgttcaat gggtggcaaa gcatctgttg agtcaatttc
     3781 aatcgccaaa ggatcgcgtt gcactcgtcc gctgattgcg gcgatggctt ccggccaggt
     3841 tgccgaaaac agaagcgtct gtcgagatgc aggcgcaaaa cggatgacat catcaatggc
     3901 atcgctaaat cccatatcca gcatgcggtc ggcctcatcc atcaccaaag tattcagcgc
     3961 atccagtgat accgtgcctt tttgcaggtg atccagcaaa cggcccggcg ttgccacgat
     4021 aatatgcggc gcatgttgca acgaatcacg ctgcataccg aacggttgac caccgcacaa
     4081 cgtcaaaatt ttggtatttg gcagaaaacg cgccagccga cgcaattcac ctgccacctg
     4141 atccgccagt tcacgcgtag gacacagcac taaagcctgg gtttgaaata gcgacgcatc
     4201 aatttgctgt aacaagccga ggccaaaagc cgccgttttg ccgctgccag ttttcgcctg
     4261 cacgcgaaca tcttttccgg caaggatcgc cggaagcgcg gcggcctgca ccggcgtcat
     4321 ggttaaataa cccaactcat taaggttcgt gagttgggcg ggaggcaaaa cattcagggt
     4381 agaaaaagcg gtcacaatct attctcgtgg tcatcgacgc aaagtcatca ggcgcgtatc
     4441 ctcgcagatc tacgctcacg atgcgacaat ttaatcggtt cttcatcggg tggtgggtca
     4501 ggcatgggtt gcgggcgagg gatcggatcg ggcactggaa caggatcgcc aggaatcggt
     4561 tcagggacag gaatttgcaa ataaataagt gtcgtcatat ttccctccgg tcattgggtg
     4621 gactcttaaa gggtagacgc tgataaataa caggcaaaaa aaagccgact catcaaagtc
     4681 ggcgtcgtac gaatcaattg tgctatgcag taattcaaaa aaggaagtaa gacaatatgg
     4741 agcgcaacgc ccatcgcttg acgttgcatt cacctgcaag agagatattg ccctgaatgg
     4801 gtagagagtt tattgacttc gctcaaactt tgcggcgttt ttgtatacag acagccggaa
     4861 aaattgcttt tgttacaacc atttactacg atgcaaccat aaagcaacac caccaataag
     4921 aacaactaac agaatacaaa aaattgaaaa tccgaattgc cacccgccgc cagggatccc
     4981 accaaggttg acgccaaata accctgtcag aaaggtactg ggtaaaaaga ccattgccat
     5041 caatgacatt gtataggtac gacgagctaa attttcctgc atcacctgag cgatttcatc
     5101 cgccatcaca ccagtccgtg ctatacaggc gtcgatttcg tcaaggccgc gcccaaggcg
     5161 atcggcaata tcctgcatcc gacggcgttg gtcatcgctc atccacggca aacgttcact
     5221 ggcaagacga gcataaacat cacgttgcgg tgccatatag cgacgcatca caattaattg
     5281 tttgcgcagc agagccagga atccacgcgg cggaatttgc tgatcaagga gattatcttc
     5341 aaggtcgata attttatcgt gcagctgctc gataaattcg ctggaatgat cggtcaacgc
     5401 atcgcacaca tccaccagcc atcccccgca atcggtcgga cccgtgccct cttccagatc
     5461 actcaccaca tcgtccagcg ccagcacttt gcgttgtcgg gtcgaaacaa ttaaccgccc
     5521 gtccatatat acacgcatgg cgaccagttg atcggggcgt tcatcggtgc tgccgtttat
     5581 acagcgcaat gtaatcagcg tgccttcacc gagacggctg actcggggcc gtgtgctctc
     5641 gcccgccagc gcatcacgta cgttattggg aagcagcggt gttgtcgcca gccattgggc
     5701 gctatcatgg tgtacataat taaggtggag ccagcaggga tgcgcttcat caatcacatc
     5761 tgtattttcc agcggtttaa cgccgcctct accatccagc atccaggcaa atactgcatc
     5821 cgggacatta acgtccgatc ccttaatcgc ttccacagtg cctccatcat caacgcatta
     5881 ttttgtagtc tagccttctg gccctgttac gcaacatctc atcaccccat taccctgaaa
     5941 tgattaataa aattctgtct aaattgaata caaaaagcaa aatgcttttc cgtat
//
LOCUS       DBFIFM010000241         5963 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.241, whole genome shotgun sequence.
ACCESSION   DBFIFM010000241 DBFIFM010000000
VERSION     DBFIFM010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.241"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..1241
                     /gene="pdeC"
                     /locus_tag="N7C74_004247"
     CDS             <1..1241
                     /gene="pdeC"
                     /locus_tag="N7C74_004247"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HGU2433996.1"
                     /translation="TIVSADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVPRNGYLYATVY
                     STKRPIAAIVATSYQRLIAHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(1244..1567)
                     /gene="soxS"
                     /locus_tag="N7C74_004248"
     CDS             complement(1244..1567)
                     /gene="soxS"
                     /locus_tag="N7C74_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HGU2433997.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1653..2117
                     /gene="soxR"
                     /locus_tag="N7C74_004249"
     CDS             1653..2117
                     /gene="soxR"
                     /locus_tag="N7C74_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HGU2433998.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2663..4012
                     /gene="ghxP"
                     /locus_tag="N7C74_004250"
                     /gene_synonym="yjcD"
     CDS             2663..4012
                     /gene="ghxP"
                     /locus_tag="N7C74_004250"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HGU2433999.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            4163..5812
                     /locus_tag="N7C74_004251"
     CDS             4163..5812
                     /locus_tag="N7C74_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HGU2434000.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
BASE COUNT         1283 a         1417 c         1636 g         1627 t
ORIGIN      
        1 atacgattgt atcggccgat tataagcgtg aacctaatgt ttctatctat tattaccgcg
       61 atacgccttt tttctctggc tataaaatga cctatatgca gcggggaaat tatgtggcgg
      121 ttatcaaccc tctcttctgg agtgaagtga tgtctgatga cccgacattg caatggggtg
      181 tgtatgatac ggtgacgaaa acctttttct cgttaagcaa agaggcctca gcagcaacgt
      241 tttctccgct gattcatttg aaggatttaa ccgtaccaag aaatggctat ttatatgcga
      301 cagtttattc gacaaaacgc ccaattgcag ccattgttgc gacttcatat caacgtctta
      361 tagcccattt ttataatcat cttatttttg cgttgcccgc cggtattttg gggagtcttg
      421 ttctgctatt actctggcta cgtattcgac aaaactattt gtctcccaaa cgcaaattgc
      481 aacgcgccct cgaaaaacat cagctatgcc tttattacca gccaataatt gatatcaaaa
      541 cagaaaaatg tatcggcgct gaagcgttgt tacgttggcc tggtgagcag gggcaagtaa
      601 tgaatccggc agagtttatt cctctggcag aaaaggaggg gatgatagaa caggtaactg
      661 attatgttat tgataatgtc ttccgcgatc tgggcgcgta ccttgcaaca catgctgatc
      721 gctatgtttc tattaatctg tcggcctccg attttcatac gtcacggttg atagcgcgaa
      781 tcaatcagaa aacagagcaa tacgcggtgc gtccgcagca aattaaattt gaagtgactg
      841 agcatgcatt tcttgatgtc gacaaaatga cgccgattat tctggctttc cgccaggctg
      901 gttacgaagt ggcaattgat gattttggta ttggctactc taacttgcat aaccttaaat
      961 cattgaatgt cgatattttg aaaatcgaca aatcgtttgt tgagacgctg accacccata
     1021 aaaccagtca tttgattgct gaacatatca tcgagctggc gcacagcctg gggttaaaaa
     1081 cgatcgctga aggcgtcgaa actgaggagc aggttaactg gctgcgcaaa cgcggcgtgc
     1141 gctattgcca gggatggttc tttgcgaagg cgatgccgcc gcaggtgttt atgcaatgga
     1201 tggagcaatt acccgcgcgg gagttaacgc gcgggcaata aaactacagg cggtggcgat
     1261 aatcgctggg agtgcgatca aactgccgac ggaaaacgcg ggagaaggtc tgctgagaga
     1321 cataacccag gtccattgcg atatcaaaaa tcggacgctc ggtggtgcgc aactcaacgg
     1381 cggccagtaa cagacggcgt tgacgaatgt aatcgccaag cgtctgatgc gtcaccgtgc
     1441 ggaacatccg ttgcaggtac cactttgaat agcctgattt ttttgcgacg acatcaatgt
     1501 taagcggctg gtcaatatgc tcgtcaatcc atgcgataag atcctgaata attttctgat
     1561 gggacataaa tctgcctctt ttcagtgttc agttcgttaa ttcatctgtt ggggagtata
     1621 attcctcaag ttaacttgag gtaaagcgat ttatggaaaa gaaattaccc cgcattaaag
     1681 cgctgctaac ccccggcgaa gtggcgaaac gcagcggtgt ggcggtatcg gcgctgcatt
     1741 tctatgaaag caaagggttg attaccagta tccgtaacag cggcaatcag cggcgttata
     1801 aacgtgatgt gttgcgatat gtcgcaatta tcaaaattgc tcagcgtatt ggcattcctc
     1861 tcgcgaccat tggtgaagcg tttggcgtgt tgcccgaagg gcatacgtta agtgcgaaag
     1921 agtggaaaca gctttcgtcc caatggcgag aagagttgga tcggcgcatt cataccttag
     1981 tggcgctgcg tgacgaactg gacggatgta ttggttgtgg ctgcctttcg cgcagtgatt
     2041 gcccgttgcg taacccgggc gaccgcttag gagaagaagg taccggcgca cgcttgctgg
     2101 aagatgaaca aaactaaagc gccacaaggg cgctttagtt tgttttccgg tctttgtctt
     2161 tctctctatc ccgctggtac acaggagggt ttcccccgac gtcaacacac ctcattcgag
     2221 cacgtggtgg aggttccggt tggtgttgat gctttaattg tatgtcaccg acgtttcttc
     2281 gccagtgtaa aagtatactt tttagccgca atatttttgt catctcagac gattttttat
     2341 cgcaatcctc aacggtatac ggctcgataa cgctgcaatc ttgcgcatcg acgataacgt
     2401 ttgcgcatca attgcctggt ttttcatcgt caagacaata aaagagaaaa aagcagcaaa
     2461 cttcggttga aaaagccgct atgatcgccg gataatcgtt tgcttttttt accacccgtt
     2521 ttgtatgcgc ggagctaaac gtttgctttt ttgcgacgca gcaaattgtc gcaaacctgg
     2581 agcaggaaga taacgtttcg ctggcagggg attgtccgcc acgcatcttg acgaaaatta
     2641 aactctcagg ggatgttctc ttatgtctac gccatcagcg cgtaccggcg gttcactcga
     2701 cgcctggttt aaaatttcac aacgtggaag cactgtccgt caggaagtgg ttgccgggtt
     2761 aacaacgttt ctggcgatgg tctactcggt tatcgtcgtt ccaggcatgt tgggtaaagc
     2821 gggcttcccg cctgcggcag ttttcgttgc gacctgtctg gttgccggac tcggttctat
     2881 cgtgatgggt ctgtgggcta atctgccgtt ggcgattggt tgcgccatct ccctgacggc
     2941 gtttaccgca ttcagcctgg tgctggggca acatattagc gtacctgtcg cgctgggtgc
     3001 cgtgttcctg atgggtgtgc tgtttacggt aatttctgcc acgggtatcc gtagctggat
     3061 tttgcgcaac ttgccgcacg gtgtggcgca cggcacgggg atcggtatcg gtctgttcct
     3121 gctgctcatt gccgctaacg gtgtcggtct ggtgattaaa aacccgcttg atggtctgcc
     3181 cgttgcgctg ggtgatttcg cgaccttccc ggtgattatg tcactggtag gtctggcggt
     3241 gatcatcggc ctggaaaaac tgaaagtccc tggtggcatt ctgctgacca ttatcggtat
     3301 ctcaattgtc ggtttgatct tcgatcctaa cgtccatttc tccggcgttt tcgccatgcc
     3361 ttcattgagc gatgaaaacg gcaattcact gattggcagc ctggacatta tgggcgcgct
     3421 gaatcctgta gtcctgccaa gcgttctggc gctggtgatg acggcagtat ttgatgccac
     3481 cggaactatc cgcgctgtcg ccggccaggc gaacctgctg gataaagatg ggcagatcat
     3541 cgacggcggg aaagcactga ccactgactc catgagcagc gttttctctg gcctggtggg
     3601 tgcagctccg gcagcggtat acatcgagtc tgcggcgggt acggcggcgg gcggtaaaac
     3661 cggtttgacg gctatcaccg ttggcgtgct gttcctgctg attctgttcc tctctccgct
     3721 ttcttacctc gttccggggt atgcaacggc tccggcgctg atgtacgttg gcctgctgat
     3781 gctgagcaac gtggcgaaaa tcgactttgc tgattttgtt gatgcgatgg cgggtctggt
     3841 tacggcggta ttcatcgtgc tgacctgtaa catcgtaaca ggcatcatga tcggcttcgc
     3901 gactctggtg attggtcgtc tggtttccgg tgaatggcgc aagttgaaca tcggtacggt
     3961 cgttatcgcc gtggcgctgg tgacattcta tgcgggtggc tgggctatct aatctttcat
     4021 cggattctga aaacgggtgg caatggctgc ccgtttttat tttctccgca cattgtgtga
     4081 gcttttgcga tattctgaaa aaatgagaat tcaggcataa cgtctttctc agatcataaa
     4141 aaacatcgca aacagggaac gcatggaaat cttcttcacc atactgataa tgaccctcgt
     4201 ggtctcgctg tccggggtgg tcactcgtgt catgcccttt cagatcccgc ttccgcttat
     4261 gcaaatcgcc atcggtgcgc tactggcgtg gccgacgttt ggtttgcatg tggagtttga
     4321 tcctgaactc tttttagtct tgtttatccc gccgttgctg ttcgctgatg gctggaaaac
     4381 gccgacccgt gaatttctcg aacatggtcg agagattttc ggcctcgcgc tggcgctggt
     4441 ggtggtcacc gtggtcggca ttggcttcct tatttactgg gtggtgccgg gcattccgct
     4501 gatccccgcc tttgcgctgg cggcggtgct ttctccgacc gatgctgtgg cgctctccgg
     4561 gattgttggc gaagggcgca tcccgaaaaa aatcatgggc attttgcagg gcgaagcgtt
     4621 gatgaatgac gcctccggcc tggtgtcgtt gaagtttgcc gtggcagtgg cgatggggac
     4681 gatgatcttc accgtcggcg gtgcgacggt cgaatttatg aaagtagcca ttggcggtat
     4741 tctcgccggt tttgtggtga gctggctgta cggtcgctcg ctgcgattcc tcagccgctg
     4801 gggcggtgat gaacccgcga cgcagatcgt cctgctgttc ctgctgccat tcgcttctta
     4861 tctgattgcc gaacatattg gcgtttccgg catcctcgct gcggttgccg ccgggatgac
     4921 catcacccgc tccggtgtga tgcgccgtgc gccgctggca atgcgcctgc gtgcaaacag
     4981 cacctgggcg atgctggaat ttgtctttaa cggcatggtg ttcctgctgt taggtctgca
     5041 gctgccgggt attctggaga cgtcgctgat ggcagcagaa atcgacccta acgtcgaaat
     5101 ctggatgctg tttaccgata ttattctgat atatgcggcg ctgatgctgg tccgtttcgg
     5161 ctggctgtgg acgatgaaaa agttcagcaa ccgcttcctg aagaagaagc cgatggagtt
     5221 tggttcgtgg accacacgag aaatcctgat cgcgtctttc gccggggtgc gtggggcgat
     5281 cactttggcc ggtgtgctct ctatcccgct gctcttgccg gatggtaacg tcttcccggc
     5341 gcgctatgag ctggtgtttc tggcggctgg tgtcattctc ttctcgctgt ttgtcggcgt
     5401 ggtgatgttg cctattctgc tacaacacat tgaagtcgcg gaccattcgc aacaattgaa
     5461 agaagaacgt attgcgcgag cggcaacggc agaagtggcg attgtggcga tccagaaaat
     5521 ggaggagcgt ctggcggcgg ataccgaaga gaatatcgat aaccagctgc ttacggaggt
     5581 cagttctcgc gtcattggta acctgcgtcg tcgcgccgat ggacgtaacg acgttgaaag
     5641 ttccgtgcag gaagagaacc ttgagcgtcg cttccgtctg gcggcattgc gttctgaacg
     5701 tgctgaactg taccacctgc gcgccacgcg ggagatcagc aacgaaacgc tgcaaaaatt
     5761 actgcacgat ctcgatttgc ttgaagcgtt gctaattgag gaaaatcagt aagccgaaag
     5821 cgacgtaacc aggcccgagg taatctgaaa aaggtgcaat cttttgcacc tttaacaggt
     5881 tagctcgggc taacattcat gattctaaaa caaataagtt atctctcttt taaagtcatt
     5941 tcattggctg ttcttcgact taa
//
LOCUS       DBFIFM010000242         5943 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.242, whole genome shotgun sequence.
ACCESSION   DBFIFM010000242 DBFIFM010000000
VERSION     DBFIFM010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.242"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            55..912
                     /gene="focA"
                     /locus_tag="N7C74_004252"
     CDS             55..912
                     /gene="focA"
                     /locus_tag="N7C74_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HGU2434001.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            967..3249
                     /gene="pflB"
                     /locus_tag="N7C74_004253"
     CDS             967..3249
                     /gene="pflB"
                     /locus_tag="N7C74_004253"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGU2434002.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3441..4181
                     /gene="pflA"
                     /locus_tag="N7C74_004254"
     CDS             3441..4181
                     /gene="pflA"
                     /locus_tag="N7C74_004254"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HGU2434003.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            4280..4390
                     /locus_tag="N7C74_004255"
                     /pseudo
     CDS             4280..4390
                     /locus_tag="N7C74_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(4391..5821)
                     /gene="ycaM"
                     /locus_tag="N7C74_004256"
     CDS             complement(4391..5821)
                     /gene="ycaM"
                     /locus_tag="N7C74_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGU2434004.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYTPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
BASE COUNT         1588 a         1505 c         1406 g         1444 t
ORIGIN      
        1 atctataatg ctttgttagt atctcgtcgc cgacttaata aagagagagt tagtgtgaaa
       61 gctgacaacc cttttgatct tttacttcct gctgcaatgg ccaaagtggc cgaagaggcg
      121 ggtgtctata aagcaacgaa acatccgctt aagactttct atctggcgat taccgccggt
      181 gttttcatct caatcgcatt cgtcttctat atcacagcaa ccactggcac aggcacaatg
      241 cccttcggca tggcaaaact ggttggcggc atttgcttct ctctgggact gattctttgt
      301 gttgtctgcg gagccgatct ctttacttcc acagtgttga ttgttgttgc taaggcgagt
      361 gggcgcatca cctggggtca gttggctaaa aactggctaa atgtctattt tggcaacctg
      421 gtcggcgcac tgctgtttgt acttttaatg tggctttccg gcgagtatat gaccgcaaat
      481 ggtcaatggg gactaaacgt cctacaaacc gccgaccaca aagtgcacca tacttttatt
      541 gaggccgtct gccttggtat cctggcaaac ctgatggtat gtctggcagt atggatgagt
      601 tattctggcc gcagcctgat ggacaaagcg tttatcatgg tgctgccggt cgcgatgttt
      661 gttgccagcg gttttgagca cagtatcgca aacatgttta tgatcccgat gggtattgta
      721 atccgcgact tcgcatctcc ggaattctgg accgctgtcg gttctgcacc ggaaaatttt
      781 tctcacctga ccgtgatgaa cttcatcact gataacctga ttccggttac gatcggtaat
      841 attatcggcg gtggtttgtt ggttgggttg acatactggg tcatttacct gcgtgaaaac
      901 gatcaccatt aatggttgtc gaagtacgca gtaaataaaa aatccactta agaaggtagg
      961 tgttacatgt ccgagcttaa tgaaaagtta gccacagcct gggaaggttt taccaaaggt
     1021 gactggcaga atgaagtaaa cgtccgtgac ttcattcaga aaaactacac tccgtacgag
     1081 ggtgacgagt ccttcctggc tggcgctact gaagcgacca ccaccctgtg ggacaaagta
     1141 atggaaggcg ttaaactgga aaaccgcact cacgcgccag ttgacttcga caccgctgtt
     1201 gcttccacca tcacctctca cgacgctggc tacatcaaca aagcgttgga aaaagttgtt
     1261 ggtctgcaga ctgaagctcc gctgaaacgt gctcttatcc cgttcggtgg tatcaaaatg
     1321 atcgaaggtt cctgcaaagc gtacaaccgc gaactggacc cgatgatcaa aaaaatcttc
     1381 actgaatacc gtaaaactca caaccagggc gtgttcgacg tttacactcc ggacatcctg
     1441 cgttgccgta aatccggtgt tctgaccggt ctgccagatg cttatggccg tggccgtatc
     1501 atcggtgact accgtcgcgt tgcgctgtac ggtatcgact acctgatgaa agacaaatac
     1561 gctcagttca cctctctgca ggctgatctg gaaaacggcg taaacctgga acagactatc
     1621 cgtctgcgcg aagaaatcgc tgaacagcac cgcgctctgg gtcagatgaa agaaatggct
     1681 gcgaaatacg gctacgacat ctctggtccg gctaccaacg ctcaggaagc tatccagtgg
     1741 acttacttcg gctacctggc tgctgttaag tctcagaacg gtgctgcaat gtccttcggt
     1801 cgtacctcca ccttcctgga tgtgtacatc gaacgtgacc tgaaagctgg caagatcacc
     1861 gaacaagaag cgcaggaaat ggttgaccac ctggtcatga aactgcgtat ggttcgcttc
     1921 ctgcgtactc cggagtacga tgaactgttc tctggcgacc cgatctgggc aaccgaatct
     1981 atcggtggta tgggcctcga cggtcgtacc ctggttacca aaaacagctt ccgtttcctg
     2041 aacaccctgt acaccatggg tccgtctccg gaaccgaaca tgaccattct gtggtctgaa
     2101 aaactgccgc tgaacttcaa gaaattcgcc gctaaagtgt ccatcgacac ctcttctctg
     2161 cagtatgaga acgatgacct gatgcgtccg gacttcaaca acgatgacta cgctatcgct
     2221 tgctgcgtaa gcccgatgat cgttggtaaa caaatgcagt tcttcggtgc gcgtgcaaac
     2281 ctggcgaaaa ccatgctgta cgcaatcaac ggcggcgttg acgaaaaact gaaaatgcag
     2341 gttggtccga agtctgaacc gatcaaaggc gatctcctga actacgatga agtgatggag
     2401 cgcatggatc acttcatgga ctggctggct aaacagtaca tcactgcact gaacatcatt
     2461 cactacatgc acgacaagta cagctacgaa gcctctctga tggcgctgca cgaccgtgac
     2521 gttatccgca ccatggcgtg tggtatcgct ggtctgtccg ttgctgctga ctccctgtct
     2581 gcaatcaaat atgcgaaagt taaaccgatt cgtgacgaag acggtctggc tatcgacttc
     2641 gaaatcgaag gcgaataccc gcagtttggt aacaacgatc cgcgtgtaga tgacctggct
     2701 gttgacctgg tagaacgttt catgaagaaa attcagaaac tgcacaccta ccgtgacgct
     2761 atcccgactc agtctgttct gaccatcact tctaacgttg tgtatggtaa gaaaacgggt
     2821 aacaccccag acggtcgtcg tgctggcgcg ccgttcggac cgggtgctaa cccgatgcac
     2881 ggtcgtgacc agaaaggtgc tgtagcgtct ctgacttccg ttgctaaact gccgtttgct
     2941 tacgctaaag atggtatctc ctacaccttc tctatcgttc cgaacgcact gggtaaagac
     3001 gacgaagttc gtaagaccaa cctggctggt ctgatggatg gttacttcca ccacgaagca
     3061 tccatcgaag gtggtcagca cctgaacgtt aacgtgatga accgtgaaat gctgctcgac
     3121 gcgatggaaa atccggaaaa atatccgcag ctgaccatcc gtgtatctgg ctacgcagta
     3181 cgtttcaact cgctgactaa agaacagcag caggacgtta ttactcgtac cttcactcaa
     3241 tctatgtaat tagatttgac tgaaatcgta cagtaaaaag cgtacaataa aggctccacg
     3301 aaagtggggc cttttttagc acgtgagcct tttttgtcag ctatctatac tttaaggtga
     3361 ctgccaaaac agactcgacg tagccttcga gctgcgcacc aacacggcct cagatgggcc
     3421 acatctggag aaacaccgca atgtcagtta ttggtcgcat tcactccttt gaatcctgtg
     3481 gaaccgtaga cggcccaggt attcgcttta tcaccttttt ccagggctgc ctgatgcgct
     3541 gcctgtattg tcataaccgc gacacctggg acacgcatgg cggtaaagaa gttaccgttg
     3601 aagatttgat gaaggaagtg gtgacctatc gccactttat gaacgcttcc ggcggcggcg
     3661 ttaccgcatc cggcggtgaa gcaatcctgc aagctgagtt tgttcgtgac tggttccgcg
     3721 cctgcaaaaa agaaggcatt catacctgtc tggacaccaa cggttttgtt cgtcgttacg
     3781 atccggtgat tgatgaactg ctggaagtaa ccgacctggt aatgctcgat ctcaaacaga
     3841 tgaacgacga gatccaccaa aatctggttg gagtttccaa ccaccgcacg ctggagttcg
     3901 ccaaatatct ggcgaataaa aatgtgaagg tgtggatccg ctacgttgtt gtcccaggct
     3961 ggtctgacga tgacgattca gcgcatcgcc tcggtgaatt tacccgtgat atgggtaacg
     4021 ttgagaaaat cgaacttctc ccctaccacg aactgggcaa acacaaatgg gtggcgatgg
     4081 gtgaagaata caaactcgat ggtgttaaac caccgaagaa agaaaccatg gaacgcgtga
     4141 aaggtattct tgagcagtac ggtcataagg taatgttcta atttccccgt aaagcggcaa
     4201 ctttattgag ttgccgcttt tttattccgc gctgcataaa taattcagag ctctatttct
     4261 aacgccctta cagcacagct tccaaatgta ttacccaaac cgccagtata tgtcctgcgg
     4321 cttgcgcgca tttctcgatt atgtaaaaac gggtcagatc tgccagagtc agcgtcaccg
     4381 accacaataa ttatttacta ttcgctttac gcgccagcag cgggaatatc agtcctaatc
     4441 ctaccagcac aaacggcgtt gcaacattca gcgccaactg gaaggtccac tctgcagtga
     4501 aggcttccat tttcgggaag atccccgtca aacaggcaaa ggcagtaaag gcaaaacacc
     4561 aaatcccgac ggtcattgcc agaggcttat tacgaataaa gacatactct ggtgtatatt
     4621 tctgcgccag gcgaacgact gcaataaatg caacaaatac ccataaataa cgcagcggca
     4681 tcactaccga gttaagattc aacagccatt tatagagatt gttcatatca ccgatgccga
     4741 gagtcggcag cataatcagg atcgccacca gcaccagggt cagaaaatag ccattaacgg
     4801 gcgtaccaga agcgttggta cgacataaac tggctggaat atatttgctg tcagcatcgc
     4861 ctaatagcac tttaagcggg gcatcaatcg agaataccag cgcagctact tgtcccaggg
     4921 tattcgcaat ggcgtaaatc accattaaag tattacccat gttgtaatac tcgcccagct
     4981 tctgaaaggc gtaatactga ccgttagtca ttaagtcatc cgggatatta cgcgagtcaa
     5041 acatcatccc catcgccagt gagcccagaa tggcacaaac cgcaaccatc accgccaggc
     5101 ataacatccc ttttggaaat tcttttcctg ggttgcgcgt ttgattaacg taaggagaaa
     5161 tcttctcagc tccgccaacc gcgaaaacca gcattgaaat agtggtaatg taggtaaagt
     5221 cgatatgagg aatgaacgtt tcccaggtaa tgtttgtggt cgcaatatgc acttcagtaa
     5281 tcgcaggcgc ggttaccgcc atcgccacat acaggagcga cataacaaac atcgcaatcc
     5341 ctgccacaga accgacgatt ttcagcgatt tcataccgcg tgaagcaacc cacataaaga
     5401 agataaacag caccagcgtt aacccctgta acgctacgac tgaatattct ttgattagcg
     5461 aaccgtcgcc tttcatcgcc caaccgagcg caatcagtat tgcctggggt ttttgtgcca
     5521 gatagggaat atgcaccacc cagtaggtcc acgcggcgag gtaagccaat ccgggtccca
     5581 tcgtgtgttt aatccaggta ctgaccccgc ccttcccatc tttgaaggtc gagcctaact
     5641 gaccaacaat tagtgcataa ggtgtgaaat agagcgcaaa gataaacacc catgaaaaaa
     5701 caaccaccag cccctggttg gcatagttat taacaacgtt gccaaaaccc cagacagtga
     5761 taaaagacat cagcgcaatg ttgtaccatc gcagctgttt ttcctgaaca ttcccagcca
     5821 tagacaaatc cttcgccaaa attaaagggg catctgtaaa acgagggatt atgcgcggca
     5881 aataagaagt gcggaaatag cgaaatgcaa aattataacc tgctgcacat tttattttta
     5941 aac
//
LOCUS       DBFIFM010000243         5939 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.243, whole genome shotgun sequence.
ACCESSION   DBFIFM010000243 DBFIFM010000000
VERSION     DBFIFM010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.243"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            25..753
                     /gene="gadW"
                     /locus_tag="N7C74_004257"
     CDS             25..753
                     /gene="gadW"
                     /locus_tag="N7C74_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HGU2434005.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1116..4229)
                     /gene="mdtF"
                     /locus_tag="N7C74_004258"
     CDS             complement(1116..4229)
                     /gene="mdtF"
                     /locus_tag="N7C74_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HGU2434006.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVIYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4254..5363)
                     /gene="mdtE"
                     /locus_tag="N7C74_004259"
     CDS             complement(4254..5363)
                     /gene="mdtE"
                     /locus_tag="N7C74_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HGU2434007.1"
                     /translation="MLTACDDKSAENTAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVKLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5471..5749
                     /locus_tag="N7C74_004260"
     CDS             5471..5749
                     /locus_tag="N7C74_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434008.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5750..>5939)
                     /gene="gadE"
                     /locus_tag="N7C74_004261"
     CDS             complement(5750..>5939)
                     /gene="gadE"
                     /locus_tag="N7C74_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HGU2434009.1"
                     /translation="LCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYK
                     LKLRRMSDIVTLGITSYF"
BASE COUNT         1455 a         1485 c         1498 g         1501 t
ORIGIN      
        1 cagtatgaaa tataagggat aatcatgact catgtctgct cggtgatcct cattcgtcgt
       61 tcattcgata tttatcatga acagcaaaaa atatcgctgc ataacgagag tatcctgctg
      121 ctggagaaaa atttggcaga cgattttgcg ttttgttcac cggatacgcg acgactggat
      181 atcgatgagc tgacagtttg ccattactta caaaatattc gtcagctacc acgcaattta
      241 gggttacata gcaaagaccg tctgttaatt aaccagtcac cccccatgcc gctggtgacg
      301 gcgatttttg atagcttcaa tgaatccggg gtaaattcac cgatactgag caatatgctc
      361 tacctttcct gtttatcgat gttttctcat aagaaagaac tgatcccctt acttttcaat
      421 agcatcagca ctgtttcagg aaaagttgaa cgccttatta gctttgatat cgccaaacgt
      481 tggtatctgc gcgatatcgc ggaaagaatg tataccagcg agagtctcat caaaaaaaag
      541 ttgcaggatg aaaacacctg tttcagtaaa atattactcg cctccaggat gtcgatggcc
      601 agacgattac tcgagttacg tcaaattcct ctgcatacta ttgcggaaaa atgtggctat
      661 agcagtacgt cgtactttat aaacacattt cgacaatatt atggtgtaac gccacatcag
      721 tttgcgcaac attcgccagg taccttttcc tgacatattt tgcatttgaa tattggtcag
      781 gatctcacac ctgcttcatg tgaaactctt ccctgatgat ttctgccggg ctaccggcta
      841 gttctctttc gcagtttgcc aggcggtata ggtattttca ggaggaaaag actcggcatg
      901 tttgggatta ttaagctgac aattcatacg attacgggaa tgtgggtcag tattgtgctg
      961 ttcaaattga tgacgaatgg ctggagtggt ttctactttc agtgctgtgt actaagcctg
     1021 gtattcctga cggttagctg gttgttgagt ggtgaatggt tagcaggaaa gagtaaggct
     1081 gaaccttcac gttcaacctt actctcattt acacgttacg cttttttaaa gcgggcaaag
     1141 agatgttcca ctacaacgaa aaagaccgga acaaagtaaa ttgccagcac tgttgcggca
     1201 aacatcccgc ccatcacgcc ggtacctacc gcgttttgcg cgccagaacc ggcaccatga
     1261 ctgataacca gcggcagcac gccgagaata aaggccagag aggtcatcag gattgggcgt
     1321 aaacgcatcc gcgccgcttc gatgattgcc tctatcggcg ttttcccttc tttctgcatc
     1381 atctcaacgg caaattcgac aatcaggatg gcgttcttgg cggaaagccc gatggtggtc
     1441 agcaaaccaa cctggaagta gacgtcatta cttaagccgc gcagatcggt ggccagtaat
     1501 gcgccaacga cgcctaacgg aacaaccaac atcaccgaga acggaattga ccagctctca
     1561 taaagtgcgg cgagggcgag gaacaccacg accagtgaaa tcgcatacag cgcaggagcc
     1621 tgatttgagg ataacgcttc ctgatacgac agtccggtcc atgagtagcc gacgcctgcc
     1681 ggaagtttag cgaccaggtc tgccataaat ttcatggcgt caccggtact tttcccggcc
     1741 gccgcttcac ctaaaatctc cattgacggg atgccgttgt agcgttccag tcgcggtgaa
     1801 ccataggtcc attcggtaga cgagtaggca gaaagcggtg ccatcgtgcc agaggcgttg
     1861 cgtacatacc attggttgat gttatccggc aacatacgga acggcgtgcc tgcctggaca
     1921 tacacttttt tcacccgccc ctggttgagg aagtcgttca cgtagctgct gccgaaggcg
     1981 gtggaaattg tctggttgat atcagacagc gccacaccca ttgcctcagc tttcgcggcg
     2041 ttgacgttca ctttgaacat cggcgtatct tccaggccgt tcgggcgtac cccggtgacc
     2101 tgattcggtg attgcgctgc cagtgataac agctcgtttc gtgcctgggt tagtttttcg
     2161 tgccccaggt taccgttgtc cagcagttcc atatcaaaac ctgacgcggt acccagttca
     2221 gccaccgcgg gtaagttgaa cgggaagacg acggctttat tgatactgct tagcgcaatc
     2281 attgcccgct gaatgatcgc ggtaaccgag ttttcctcac cgacacgttc agaccacggc
     2341 ttgagactga taaacgccag accgttgttt tgcccctggc cgctgaagcc aaagccgcca
     2401 acggtaaaca ccgactgaac attatctttc tcttttgtaa gataataatc cgtcacctgt
     2461 tgcagcactt tcgtggtgtt aaccatggtg gcaccggaag gtaactgcgc ggtggtcata
     2521 aataccccct gatcctcttc tggtaagaaa gaggtcggcg tgcgcaggaa cagcaccgcc
     2581 atcccggcgc aaatcagcag gtagatcacc atgtagcgac cggtacaacg caacagtgag
     2641 cgggtgctat cggtatagtg ctgagttgat ttttcaaaca gcgtgttgaa gcgtgcgaac
     2701 agggcattag gtttgtgacc gccttccggc gcggctttca gaatggtggc gcacagggca
     2761 ggggtcaggc tcattgccac aaatactgaa agcagcatgg aggagatcag cgtgatggag
     2821 aactggcggt agatctcccc ggttgcaccg ctcataaagg ccatcggcat aaacactgcg
     2881 gaaagaacaa cggcaatacc gaccagcgca cgttggatct gccccatcga tttatgcgtc
     2941 gcttccttcg gtggtagctt atcttccgca atgacacgct cgacgttctc caccaccacg
     3001 atggcgtcat ccaccagtaa ccctatcgcc agcaccatcc cgaacatagt caacgtgttg
     3061 atggtgaaac cgaccgccga caagatcgca aacgtcccga gaataaccac cggtacggca
     3121 atcgtcggga tgattgtggc acggaaattc tgcaaaaaca gatacatgac caggaagacg
     3181 aggatgatag cctcaaccag tgttttgaaa acttcctgaa tagaaatttc gataaacggc
     3241 gtggtgtcgt aaggataaac cgtcttcaga cttgccggga aataggctga taagcggttc
     3301 agttcctctt tgactgcccg cgaggtatcc agggcgtttg ctccggtagc cagtttgatg
     3361 gcgatcccgg cagcaggttt gccgttatag cgcgccacgg tggaataatc ttccgcccca
     3421 agttcgacgc gggcgacatc acgcagcagc acttgcgaac catcttgctg aactttcaac
     3481 aggattttgc caaattcttc cggcgtttgc agacgcgtct gcacaatgat cgaggcgttt
     3541 agttgctggt ctgccgcctg tggcatgcca cccagttgac caccagaaat ctggttgttt
     3601 tgcaccttaa tctgggaaat aacatcggaa ggtaccagat tgtatttatt gagtttttgc
     3661 gggtccagcc agatacgcat ggcgtactcg gaaccaaaga gctgtacgct accaacgccc
     3721 gcggtacggc ttagcgggtc tttgatatta gacgctacat agtccgcgat atcgtactgg
     3781 ttgaggctgc cgttatcaga aataaacgcc gctaccatca ggatattact gctcgattta
     3841 tcgacgctaa tcccctgctg ctgcactgct tcaggtaatg aaggcatagc gagttgcagt
     3901 ttattttgca cctgaacctg tgcgatatca ggagatgtcc cagtctcgaa ggtcagagtg
     3961 atagaggcat tacccgccgc atcactggtt gaagacatgt acatcaggcc atcaagccca
     4021 ttcatatttt gctcaatcac ctgagtgacc gagtcttcta ccgtttgcgc atcggcaccc
     4081 ggataggtag cgctgacggt aatggtcggt ggcgcaatct gcggatactg cgcaaccggt
     4141 aagttcatga tcgccagacc acctgcaagc atcataataa tggcaagtac ccaggcaaaa
     4201 accgggcgat caataaaata gttagccatg aaagtcccct taagcctgca acgttattgt
     4261 ttcgattcgg tgctggcgtt ttcctggctg gaggaaattg ctcgtgcttt gatacccgga
     4321 cgaatgcgtt gcaaaccgga aacgatcacc cgatcgccag cctgcaagcc agaggtgaca
     4381 acccactggt cgccgatggc tttgctggct tcaatttcgc gtagcttcac gacatcgtct
     4441 ttatccagaa tgagcgccgt tgctttacct tgggcgttgt gggtgacgcc ttcctgcggc
     4501 accagtaata cattctggcg gctaccttca tccactaatg ccgtgacata catgccaggc
     4561 agcaagtcac catttgggtt ggggaaaatc gcccgtaacg tcacggagcc cgtggtttca
     4621 tcaaccgtcg ggtcggagaa tttcagcgtg ccggtctggc tgtaacgttt accattttcc
     4681 agattgagct gtactggcgt actgccctga acctgtttga tttgcccact ggcgacctct
     4741 tctttcatgc gtaagaaatc ttgcaccgac tgcgtgagat cgacataaat cgggtccaga
     4801 cgttgtacgg taaccagcga atctgcctga ttagcggtaa cgagtgcgcc gacggtcacc
     4861 gacgatttcc cgctgacgcc cgtaatcggc gaggtgacat tcgcgtattg cagattgatc
     4921 gtcgcctgtt caacagccgc tttggcgacg gtgacattgg cttctgcttc attcaactgg
     4981 gtgcgcgcgg tgtcgtaatc ctgacggcta acgtagttgg tcttcagcaa cgatgcctgg
     5041 cggttaaagg tgatgcgggc attgctggcg gtagagagcg ctttcgccag cgagcctttg
     5101 gcggagttta gctcggcctg taaaggtgca ggatcaatct gatacagcga atcgccctgg
     5161 ttcactttat cgccttcgat aaagttgcgt ttaatgataa taccgcccac ctggggacgt
     5221 atctcggcaa cttcataagg aacggttcta ccgggcaatt cgctcaacac attgaccgac
     5281 ccaggggaga gtgtgacgac accgacctca ggcgtcatgg cggcggtgtt ttccgccgat
     5341 ttgtcatcgc aggcggtgag catcgcgccg cagaataaca acggtattaa cagctttctt
     5401 cttctgttca ttttagtccc tgaaaattct tgagaaataa cggttcttta aattgcgcgc
     5461 gaggagagat atgagggatt tacagaccag cggcatagtg gggttgtcag ccagtaaggt
     5521 gggatacagg catagtgatc gacatggtga ggtcaacgac aaaaaaaatg ttgccgttct
     5581 gccaacagtt cctgccagca ttcgggcaac gaagggttct actggtggat acacatacca
     5641 ggggaataaa gtcgttttag cctctccttt agtaatcacc gggggtaatg aaatcagcat
     5701 ctgcatccct cgtcatgcca gccatcaatt tcagttgctt atgtcctgac taaaaataag
     5761 atgtgatacc cagggtgacg atgtcgctca tacgtcttag tttcagcttg tacataatgt
     5821 tgaatttctg aatctttaat gtttgttgcg aaatattgag cgtcgacgtg atattgcttt
     5881 catttttatt tttcagaatc agttgcataa ttttgaactg attatgtgaa aaacataat
//
LOCUS       DBFIFM010000244         5835 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.244, whole genome shotgun sequence.
ACCESSION   DBFIFM010000244 DBFIFM010000000
VERSION     DBFIFM010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.244"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(160..447)
                     /locus_tag="N7C74_004262"
     CDS             complement(160..447)
                     /locus_tag="N7C74_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU2434010.1"
                     /translation="MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLS
                     EHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIEIVSVTHSRRQFPFSI"
     gene            complement(447..638)
                     /locus_tag="N7C74_004263"
     CDS             complement(447..638)
                     /locus_tag="N7C74_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HGU2434011.1"
                     /translation="MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDE
                     VMAEMENLIAQIAVTNKSE"
     gene            complement(666..1067)
                     /locus_tag="N7C74_004264"
     CDS             complement(666..1067)
                     /locus_tag="N7C74_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2434012.1"
                     /translation="MMSALEVSMYRISKLLQETGWSQAELARRIGVTQQTVQQWVSGK
                     ATPKASSLDKLVEVSGHPLHWFLLPPEECEQIFTPDTMKIGPRQRELLQAFSAFPEED
                     QEKMLQEIKDKKKSMEETIARWLAAQKSRRA"
     gene            1176..1448
                     /locus_tag="N7C74_004265"
     CDS             1176..1448
                     /locus_tag="N7C74_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU2434013.1"
                     /translation="MLDSTREKIRQKYTQAEIGRYMGVAQQTVWQWFSFGVPPKQVIP
                     LCQLMKWEVTPHEIRPDIYPNPTDGLPVGCKVNTSNAPELIHENQA"
     gene            1432..1857
                     /locus_tag="N7C74_004266"
     CDS             1432..1857
                     /locus_tag="N7C74_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU2434014.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEAMARNTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPPLVARMRSHSSAYFRE
                     LVETKERLVKDIDDFVASAIVLFDQMNRGGPAGNTLAVH"
     gene            complement(2064..2519)
                     /locus_tag="N7C74_004267"
     CDS             complement(2064..2519)
                     /locus_tag="N7C74_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434015.1"
                     /translation="MYFLKSLYQAHVLNVAATNRWCNSPEMLPDYRAWLRAETYLRLD
                     ILISELQKETASIHNLQGIDAVRILVSRHSALSIIEVRHLSFSELIFLLQPALESANI
                     PPEVIQYPPHVDEQLQDVPYNQRAGLTPCSEAEWDHSLLKKYQDLYNPQ"
     gene            2598..3689
                     /locus_tag="N7C74_004268"
     CDS             2598..3689
                     /locus_tag="N7C74_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU2434016.1"
                     /translation="MRDYAKVSPRFWLGETGRELRKAGAEAQVVAFYLMTSPHANMLG
                     LYYLPVLYLAHETGLGLEGASKGLKRAVEAGFCSYDHDAEMVWVHEMAAWQVGETLKP
                     GDNRCAGVRNEYASLPENAFLSVFYDRYKTDFHLDVRRNNSRNSVRGFEGAFKGLRSQ
                     EQEQEQEKEQEQDKNTMVHGKKNTTNQAGDVQTVNPGQPAGTTPEADSGAVQQVMTAG
                     SEQSHQLQQPEADSAIQREADRVVPESTGQSVGRVDYPDVFEQVWREYPLRAGANPKK
                     SAFSAWKARLREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPW
                     LLPVSGTNNQRCVNHISEPDTEIPPGFRG"
     gene            3696..4442
                     /locus_tag="N7C74_004269"
     CDS             3696..4442
                     /locus_tag="N7C74_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU2434017.1"
                     /translation="MKNIAAAGVLERIRRLAPQASVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNHQLRVEKILNRSGIQPLHSKCSFANYQVQNDGLKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCSVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNMRVVK"
     gene            4464..5234
                     /locus_tag="N7C74_004270"
     CDS             4464..5234
                     /locus_tag="N7C74_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU2434018.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKINGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            5250..5663
                     /locus_tag="N7C74_004271"
     CDS             5250..5663
                     /locus_tag="N7C74_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HGU2434019.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPVVVDPDLIWSLPDG
                     EIRRYDRQLNIICSECRNSEVMQRVLIFYTGVMME"
BASE COUNT         1567 a         1218 c         1635 g         1415 t
ORIGIN      
        1 ctgcttggta actcgaagta ttgcccggcg ttctgtgggg cggggtgggt ggttggtgta
       61 tgtaatctac aataaaaaac tgtttttgtg tcaacagttt ttaattgttg ttttgggcaa
      121 aaaaatccct cgaaagaggg agtatgaaaa ttgttcaact cagatagaga agggaaattg
      181 tcgccgagaa tgtgtcacgc ttacaatctc aatgcttgaa gcagctactc tgtacaggat
      241 tatgtagtta gggtgggtca cgatctctct caatccagaa acccgttcgc ttggtggata
      301 taagtacgga tgctcagata gaggtaatac cgatgtttca atgcgtattt ttagtctacg
      361 tgctgccggt gggttctcct tggcgatgta agttatgatc tggcgcaaat catcgcgagc
      421 agacggtagc cataaaatgg gtaacattac tcgctcttgt tagttacagc aatttgagca
      481 ataagatttt ccatttcagc cattacctcg tcatgtggaa ttgcggggcg agtgtctgca
      541 aggcttgacg ttactttagt gcgcaaccat tcgttgtaac tgttttcttg ttcggtagtt
      601 tcgaattctg aaactatcgg agaaagggct gtacccatgg cataactcct cttcttgtac
      661 tgtggtcacg cccggcggct tttttgtgcc gccagccacc tagcaatggt ttcttccatt
      721 gattttttct tgtctttgat ttcttgaagc attttttctt ggtcttcctc aggaaacgca
      781 ctaaaagctt ggagcagttc gcgttggcga ggaccaattt tcatcgtgtc aggggtgaaa
      841 atttgctcac actcttcagg aggcaataaa aaccaatgca atggatgccc tgaaacctca
      901 accagtttat ccaaacttga ggctttaggt gtagccttac cgctgaccca ttgttgaaca
      961 gtttgttgtg tcacaccaat tctacgggca agctcagcct ggctccatcc agtttcctga
     1021 agaagcttgc tgattctgta catagatact tctagggcgc tcatcattat tcaattttac
     1081 aggtaaatac tgttaaaagc atcacaataa aaaactgttg attgcataca gttttttatt
     1141 gtaggctttg cttatagttt ttagaggagg gcaaaatgct agatagcact cgcgaaaaaa
     1201 ttaggcagaa atacactcag gctgaaatag gtcgttatat gggggtcgct caacagactg
     1261 tttggcaatg gtttagcttt ggcgttcccc caaagcaggt aattccgtta tgccaactaa
     1321 tgaagtggga agttaccccg catgaaatcc gcccagatat ttatcctaac ccaaccgacg
     1381 gtttacctgt tggatgtaag gttaacacat caaatgcgcc ggagttgatt catgaaaatc
     1441 aagcatgaac acatccgcat ggcgatgaat gcctgggcgc atccggacgg cgaaaaagta
     1501 ccggctgcga aaattaccaa agcgtatttc gagctgggaa tgacgttccc ggaactgtat
     1561 gacgacagcc atccggaagc catggctcgc aatactcaga aaattttccg ctgggtggag
     1621 aaagacactc ctgatgcggt taaaaaaatt caggcgttgt taccagcgat cgaaaaagcg
     1681 atgccgcctc cgctggtggc ccgaatgcgc agccacagtt ccgcttattt tcgggagttg
     1741 gtagagacga aggaacggct ggtgaaagat attgatgatt tcgttgcatc agcgatcgtt
     1801 ctgttcgatc agatgaatcg tggtggcccg gcaggaaaca ctctggctgt gcattaattg
     1861 ggtaataaat atgagtaatg acaaaaaatt gacactgagc gtttacgaaa acagtccgca
     1921 catctggcgt ggcggtttat ctgatgtgga gctggcagag tggttgatac ataaagctaa
     1981 tgcgctgctc tggcgtttgt cagccagaga acagcgcaag gaaaccagaa taaagctggc
     2041 tgatgcagaa gcgtgtgccg ggcttattga ggattataca aatcttggta tttcttcagc
     2101 agagagtgat cccattcagc ctctgagcag ggagtcaatc cagcacgctg gttgtatggc
     2161 acatcttgta actgctcgtc aacatgaggt gggtattgga tcacttccgg cgggatattc
     2221 gctgattcca gagctggttg aagcaagaaa atcagttcag aaaaagagag atgacgcact
     2281 tcaattattg agagagcact atggcgcgat accagaatgc gaacagcgtc gataccctga
     2341 aggttatgaa tggatgcagt ctctttttga agttcgctaa tcaatatgtc gagacgaagg
     2401 tatgtttcgg cgcgcagcca ggctctgtaa tccgggagca tttcggggct gttacaccag
     2461 cggtttgttg ctgcaacatt taatacatga gcctgataaa ggcttttcaa aaaatacatg
     2521 tcgaacctcc tctggttctg tcgattggga accacagatt atatccggag gaaggttcgg
     2581 caccagatga ggtagccatg cgtgattacg caaaagtttc tccgcgattc tggctgggag
     2641 aaacggggag agaacttaga aaggcgggtg cagaagcgca agttgttgct ttttacctga
     2701 tgacatcccc tcacgcaaat atgctgggtt tgtattacct gccagtttta taccttgctc
     2761 atgaaaccgg gcttggtctg gaaggggctt caaaggggct taaaagggct gttgaagctg
     2821 gtttttgtag ctatgaccat gatgcagaga tggtctgggt ccatgaaatg gcagcctggc
     2881 aggttgggga aacgttgaag cctggcgata accgttgtgc aggtgtcagg aatgagtatg
     2941 catcattacc tgaaaacgct tttctgtcag tgttttacga cagatataaa acggatttcc
     3001 atctggatgt gaggcggaat aatagccgaa attcggtaag gggcttcgaa ggggctttta
     3061 aggggcttcg aagccaagaa caggaacagg agcaggagaa agaacaggaa caggacaaaa
     3121 acactatggt tcatggcaaa aaaaacacca cgaaccaggc aggggatgtt cagaccgtca
     3181 atcctggtca gccagcaggc acgacaccgg aagccgattc gggcgctgtg cagcaggtga
     3241 tgaccgcagg gtcggagcaa tcacaccaac tgcagcagcc tgaagccgat tccgccattc
     3301 agcgggaagc cgatcgggta gtcccggaaa gcaccgggca gtctgtggga cgagtggatt
     3361 atccggatgt gttcgaacag gtctggcggg aatacccgtt gcgtgctggg gcaaacccga
     3421 agaaatccgc tttcagtgcc tggaaggcca gattgcgcga gggggtgcca ccagagacca
     3481 tgctggatgg tgtgaggcgt tacgcgagat acctggcggc gaccgggaaa gcgggaacgg
     3541 aatttgttca gcgagcgacg acgttttttg ggccggaccg gaattttgaa aacccctggt
     3601 tgctcccggt aagcggcacg aacaaccagc gttgtgtgaa tcatatttct gaaccggata
     3661 ccgaaattcc gccgggattc agggggtgat gtggcatgaa aaacattgcg gcagccgggg
     3721 ttcttgaacg tattcgcaga cttgcaccac aggcgtcggt tccaccgtac cggacggtgg
     3781 aggagtggcg ggaatggcaa cttgctgaag gacgaaaacg cagcgaggag attaaccgcc
     3841 agaatcacca gttgcgggtg gaaaaaatcc tgaatcgttc gggcatccag cctctgcaca
     3901 gcaaatgctc gtttgcgaat tatcaggtgc agaacgacgg gctaaaatac gcgctgagcc
     3961 aggccaaatc catagctgac gaactgatga ccgggtgcac gaattttgtg ttcagcggta
     4021 aaaccggcac cgggaaaaat caccttgcag cggcgatggg taaccggctg atggcgaagg
     4081 ggcgcagcgt gattatcgtc accgtgtctg atgtcatgag cgtgttgcat gacagctacg
     4141 acaacggcaa atccggggaa aaatttttac aggagctttg cagtgttgat ttgctggtcc
     4201 tggatgaaat aggcgttcag cgggagacga aaaacgagca ggtggtatta caccagataa
     4261 ttgatcgccg gacagcatca ctgtgcagtg tcgggatgtt aacaaacctg aatcatgccg
     4321 caatgagtac acttcttggt gagaggatta tggaccgcat gaccatgaac ggtggtcgat
     4381 gggtgacgtt taactgggat agctggcgtc caaatgtcag caatatgagg gttgtgaagt
     4441 aattttgtcc ggaggaaatt ttaatggaaa ccgtatctga cgcactgaaa gcactgaaaa
     4501 aagcctcttc acatgtggtg gcagctcgcc ttggaatcag tcgtgaagag gctgtcaacg
     4561 agctgtggga actcaaaata aatggcgtcg ttgataaaac tggtcacacc tggtttctgg
     4621 ctggcgaagg tgaatcccgg gtaaccgaag agcggccagt aaaatctgaa gcacaggata
     4681 tgctgaccgg ggaggtcgaa caaaaagtta ccgcagacat gatgattgag tttatcggtc
     4741 aggatggggc taaaacgtgt gaggaactgg cgggtaagtt cggtgtcagt actcgcaagg
     4801 ttgcttccac gctggcggtg gtaaccgcaa cggggcggct ggcacgcgtt aatcagaacg
     4861 gtaaatttcg ttactgcatg ccgggcgata atttaccagc agagccgaaa gccgcgctgg
     4921 taacggaaag tgatggtaag gcctttcctc agccagcagg tgctgcgtta ccagtccggg
     4981 aagccgcaac acaggaagaa attaaaacag aaactgtggc ggacattgtg cagccgttgc
     5041 catcgtttac cgaaacgcaa gcagatgagc tgatttttcc gtcccttcgc agggcaaacc
     5101 tggcgctgcg cagggcgaaa agtgatgttc agaagtggga gcgagtctgc gccgcgctgc
     5161 gggagctgaa caagcaccgg gatattgttc gacagattac tgattcttcc cgccgtgttg
     5221 tatcggaaaa gtgattgccg gaggcgctta tggcaaaagt atttacacag gaagagcggg
     5281 aaaaaattaa agggcaggtt gttgagctag tacgccggag tgggcgcgag acgttacggc
     5341 aactggaagc caagacaggt gcgacaagat atctgatgag cgttcttgcc agagagctgg
     5401 ttgccagtgg cgatgtatac aactctggtt acgggttatt cccatctgaa caggctcgta
     5461 aggactggca aaacgcccgc aaaaaactat cgagggcaaa actgaagaaa ccggttgtgg
     5521 ttgatcctga ccttatctgg tcattaccag acggagaaat acgccgctat gacaggcaat
     5581 tgaacataat ttgtagtgag tgccgtaaca gtgaagtgat gcagcgagtt ttgatatttt
     5641 acacaggagt aatgatggaa tagtgaaaat aacatgaaac ctttggattc tggtgtttca
     5701 gtggatggga aaagagcaga tacatagaaa atgaatagca ataattcaca gtctggatgt
     5761 tgtttgtatg cttataaaat gatcctagta tgcattgccg gagttgaatc agctccaatg
     5821 aagaaactga gagga
//
LOCUS       DBFIFM010000245         5804 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.245, whole genome shotgun sequence.
ACCESSION   DBFIFM010000245 DBFIFM010000000
VERSION     DBFIFM010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.245"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            3..866
                     /gene="yjeJ"
                     /locus_tag="N7C74_004272"
     CDS             3..866
                     /gene="yjeJ"
                     /locus_tag="N7C74_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HGU2434020.1"
                     /translation="MSIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELRDL
                     LIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNTLE
                     LTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSLLD
                     FLPLYDVDCQQNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKTRE
                     ATPGKEIEAITRRMLDFSPRLKKLVGVPCQVYVRTVAANNAQPLTQDQCLRALHHLRV
                     QSTSKTAPQAK"
     gene            complement(1059..1412)
                     /gene="yjeI"
                     /locus_tag="N7C74_004273"
     CDS             complement(1059..1412)
                     /gene="yjeI"
                     /locus_tag="N7C74_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HGU2434021.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1550..3196)
                     /gene="groL"
                     /locus_tag="N7C74_004274"
     CDS             complement(1550..3196)
                     /gene="groL"
                     /locus_tag="N7C74_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HGU2434022.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3240..3533)
                     /gene="groS"
                     /locus_tag="N7C74_004275"
     CDS             complement(3240..3533)
                     /gene="groS"
                     /locus_tag="N7C74_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HGU2434023.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3809..5062
                     /gene="yjeH"
                     /locus_tag="N7C74_004276"
                     /pseudo
     CDS             3809..5062
                     /gene="yjeH"
                     /locus_tag="N7C74_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
     gene            complement(5081..5557)
                     /gene="fxsA"
                     /locus_tag="N7C74_004277"
     CDS             complement(5081..5557)
                     /gene="fxsA"
                     /locus_tag="N7C74_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HGU2434024.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
BASE COUNT         1382 a         1504 c         1398 g         1520 t
ORIGIN      
        1 ccataagcat caagggtgtt aataccggtg ttattcgcaa gagtaataat ttcattgcac
       61 tggcgctgaa aattaaagaa cctcgcaata aagaatcgtt gttctttatg tctgtcatgg
      121 aactgcgcga cctgctgatt gcgctggaaa gtcgtctgca tcaaaagcat aaactggatg
      181 ctgctgcacg ccttcagtat gagcaagctc gcgataaagt cattaagaaa atggcagaaa
      241 acatcccaga aattctggtt gatgagctta aaaatgccga tattaatcgt cgggttaaca
      301 cgctggaact taccgataat caaggcgaaa atcttacctt cgtattaacg ctacatgacg
      361 gtagcaaatg tgagttagtc gtcaatgagt tgcagattga aatgctggcg cgagcaatca
      421 ttcatgcaat taataatgcg gaaatgcgtg aactggcatt acgtattacc tcactgttag
      481 atttcctgcc gttatatgac gttgactgcc agcaaaatgg caatctggaa tatgacacct
      541 attcccaacc ggaatggaaa cataacttgt tcgaccatta tctggcggtg ctttaccgct
      601 ttaaagatga aagtggcaaa gaacaattta gcggcgcagt ggtgaaaacg cgtgaagcaa
      661 cgccgggtaa agaaatagag gcgatcactc gccgaatgct tgatttcagc ccacgactga
      721 aaaaactcgt gggtgtgcca tgtcaggtct atgttcgcac cgtagcggcg aataacgccc
      781 aaccgctaac ccaggatcaa tgcttacgcg cgctgcatca cttacgtgtt caatccacca
      841 gcaaaactgc accacaggcg aaataatctc atctttgcta atgcgaggct ggagccatcc
      901 agcctgaaat gactgataac gtgatcgttg gtcatgccgg atgcggcgta aacgccttat
      961 ccggcctaca aattctcaag cattcaatca gttgcaatgc cttatgtagg cctgataagc
     1021 gaagcgcatc tggcaattat acgtttgtca ccatcacatc agttcgggca cttatatacc
     1081 tggccgataa tctggctatc cgtcgggaca aaactggaca acattccctg cgacgggcta
     1141 ctgatgccat aaatcacgtt accgcccatt gcagccgcct ggttgcgcag atcgtttgct
     1201 gcgccgcgca tagaaccgcc ctcttctccg tgttgcccgg aaagccagtt gctttgctta
     1261 cctgtcgcag taccaatcag ctggcactct gcgcctggct gctcgtccac aatgcgtaca
     1321 ctctgaccgg cagcactcaa ttcgttgctg gagctacaac ccgccatcag tagcgcggca
     1381 ccgacaatcc ctgctaagta ttttacgtgc atgttattcc ccataatcaa tgagctggac
     1441 gccactcgcg tcgtccgtgt ctgaatctta tactagaaag atgaccaaaa gaaaaacccc
     1501 cagacatttc tgcccggggg tttgtttatt tctgcgaggt gcagggcaat tacatcatgc
     1561 cgcccatgcc acccatgcca cccatgccgc cagcagcgcc taagtcagct gcatcgtttt
     1621 tcggcaggtc ggtaaccatg cattcggtgg tgatcatcag gccagccaca gaagccgcgt
     1681 actgcagagc agaacgggtt actttggttg ggtccaggat acccatgtcg atcatgttgc
     1741 cgtattcttc ggttgctgcg ttgtaaccgt agttgccgtc gccgccttta acggtgttag
     1801 caacaacaga cggttcttcg ccgcagttca ggacgatctg acgcagcgga gcttccattg
     1861 cacgcagtgc aactttgata cccacgttct ggtcttcgtt ctgaccacgc aggtcagcca
     1921 gtttagacgc tacgcggatc agcgcaacac caccaccagc aaccacgcct tcttctaccg
     1981 cagcacgggt cgcgtgcagg gcgtcttcaa cgcgtgcttt tttctctttc atttcaactt
     2041 cggtagcagc acctactttg ataactgcaa cgccgcctgc cagtttcgct acgcgctcct
     2101 gcagtttttc acggtcgtag tcagaagttg cttcttcaat ctgctgacgg atctgagcaa
     2161 cacggccctg gattgcagct tcttcgccca cgccatcgat gatggtggtg gtgtctttgt
     2221 tgatcacaac gcgtttagcc tgacccaggt cttccagggt tgctttttcc agctccatac
     2281 cgatctcttc agagattacg gtaccgccag tcagggttgc gatatcctgc agcatagctt
     2341 tacgacgatc gccgaagccc ggagctttaa ctgcagcaac tttcacgatg ccacgcatgg
     2401 tgttaacaac cagagttgcc agcgcttcgc cttctacatc ttcagcgatg atcagcagcg
     2461 gtttgcctgc tttggcaacg gcttccagaa ccggcagcat ttcgcggatg ttggagattt
     2521 tcttgtcagc cagcaggatg aacgggcttt ccagttctac tgcgccagtt tccggcttgt
     2581 tgatgaagta aggagacagg tagccacggt cgaactgcat accttcaacc acgtccagtt
     2641 cgtcctgcag accggtaccg tcttcaacgg tgataacgcc ttctttaccg actttgtcca
     2701 tcgcttcagc gatcagttta cctacggttt cgtcggagtt agcggagata gtaccaacct
     2761 gagcaatcgc tttagagtca gagcacggta cggacagcgc tttcagttct tcaactgcag
     2821 cggtaacagc tttgtcgata ccacgtttca ggtccatcgg gttcatgccc gcagcaacag
     2881 ctttcagacc ttcagtgatg atagcctgag ccagtacggt tgcagtggtg gtaccgtcgc
     2941 ctgcagcgtc gttcgcttta gaggcaactt ctttcaccat ctgcgcaccc atgttttcga
     3001 acttgtcttc cagttcgatt tcacgagcaa cggaaacacc atctttggtg atggtcggtg
     3061 caccgaaaga tttatccaga actacgttac ggcctttcgg accgagggta actttcactg
     3121 catctgccag tacgtttacg ccgcgcagca ttttcacacg agcgtcgtta ccgaatttta
     3181 cgtctttagc tgccattatc tttattcctt aaattcgtat gttcagtgtc gtgcgaggat
     3241 tacgcttcaa caattgccag aatgtcgctt tcggacatga tcaacacttc ttcattgtcg
     3301 atcttctcgg atttcacacc gtagccatcg ttgaaaataa cgatgtcgcc aactttcaca
     3361 tccagcggct tcacttcgcc attttcaagg atacggccat tgccgacagc cagcacttcg
     3421 ccgcgggtgg atttagccgc tgcagagccg gtcagaacga tgccgccagc agatttagtt
     3481 tcaacttctt tacgcttgac gatcacgcga tcatgcaatg gacgaatatt cattgataac
     3541 tctcctttga gaaagtccgt atctgttatg ggtgacgccg gagcttacgt ggtttcccgg
     3601 ctggtgacca gagaaatggg gatgaggctt cgccccttca agggggaaaa agaaaaaaaa
     3661 ttctgatcag cacaaaatcg ggtgaaaacc ctgattcacc tcacatttca tcgcaatttc
     3721 ttcatcgccg tcataagcga atctgattgt gctaccatcg aaaatctacg cagttgccca
     3781 aaatttgggc gcaatcggac atcaacccat gagtggactc aaacaagaac tggggctggc
     3841 ccagggcatt ggcctgctat cgacgtcatt attaggcact ggcgtgtttg ccgttcctgc
     3901 gttagctgca ctggtagcgg gcaataacag cctgtgggcg tggcccattt tgattatctt
     3961 agtgttcccg attgcgattg tgtttgcgat tctgggtcgc cactatccca gcgcaggcgg
     4021 cgtcgcgcac ttcgtcggta tggcgtttgg ttcgcggctt gagcgagtca ccggctggct
     4081 gtttttatcg gtcattcccg tgggtttgcc tgccgcgcta caaattgctg ccggattcgg
     4141 ccaggcaatg tttggctggc atagctggca actgttgttg gcagaactcg gtacgctggc
     4201 gttggtgtgg tatatcggta ctcgcggtgc cagttccagt gcaaatctac aaacagttat
     4261 tgccggactt atcgtcgcac tgattgtcgc tatctggtgg gcgggcgata tcaaacctgc
     4321 gagtatcccc ttccccgcgc caggaaatat cgaacttacc gggttatttg ctgcgttatc
     4381 agtgatgttc tggtgttttg tcggtctgga agcatttgcc catcttgcct cggaatttaa
     4441 aaatccagaa cgtgattttc ctcgtgcttt gatgattggt ctgctgctgg caggattagt
     4501 ctactggggc tgtacggtag tcgtcttaca cttcgacgcc tatggtgaac aaatggccgc
     4561 ggcagcatcg cttccaaaaa ttgtagtaca actgttcggt gtaggagcgt tatggattgc
     4621 ctgcgtaatt ggctatctgg cctgctttgc cagtctcaac atttatatac agagcttcgc
     4681 ccgcctggtc tggtcgcagg cgcaacataa tcctgaccac tacctggcac gcctctcttc
     4741 tcgccatatc ccgaataatg ccctcaatgc ggtgctcggc tgctgcgtgg tgagcacgtt
     4801 ggtgattcat gctttagaga tcaatctgga cgctcttatt atttatgcca atggcatctt
     4861 tattatgatt tatctgttat gcatgctggc aggctgtaaa ttattgcaag gtcgttatcg
     4921 actactggcg gtggttggcg ggctgttatg cgttctgtta ctggcaatga tcggctggaa
     4981 aagtctctat gcgctgatca tgctggcggg gttatggctg tttctgccaa aacgaaaaac
     5041 gccggaaaat ggcataacca catcatccgg cgtttcgaca ttaatcctgg cgatcgtctt
     5101 tatgatcaag gcggtcgcgg tcatcatcct ttcgctggta ctcaccatca aaagtattac
     5161 cgccaccggt cccggcgcta aaaccgccgc ctggcatgcg agaaaagcgc aaatgcggca
     5221 tcaacttcac tgtcagatgc ttttgcaccg gcggcaataa aagcagaaga ccgaggaagt
     5281 cggtgaaaaa gcccggtaat aaaagcagca aaccggcaat gatcagcgaa acacttttaa
     5341 tcatctccgc cgctgggttt tcacccgccg ccattttttg ctgcatcagc acgaaattct
     5401 taaagccctg gttacgtacc agtgacatac cgataactga cgtaaatata accagcacga
     5461 gagtcagcaa tacccccaat acatgggcaa cctgaataaa gattgaaatc tcaatataga
     5521 cataaaggaa aatggcaata aaaggtaacc agcgcaaagg tttctcctgt aatagcagcc
     5581 ggttaaaccc ggctacctga atgggttgcg aatcgcgttc agcttatatt gtggtcatta
     5641 gcaaaatttc aagatatttg cgcaactatt tttggtagta atcccaaagc ggtgatctat
     5701 ttcacaaatt aataattaag gggtaaaaac cgacacttaa agtgatccag attacggtag
     5761 aaatcctcaa gcagcatatg atctcggata ttcggtcgat gcag
//
LOCUS       DBFIFM010000246         5668 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.246, whole genome shotgun sequence.
ACCESSION   DBFIFM010000246 DBFIFM010000000
VERSION     DBFIFM010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.246"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            91..1074
                     /locus_tag="N7C74_004278"
     CDS             91..1074
                     /locus_tag="N7C74_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU2434025.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDVSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            1123..2607
                     /locus_tag="N7C74_004279"
     CDS             1123..2607
                     /locus_tag="N7C74_004279"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGU2434026.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQLVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            2600..3571
                     /locus_tag="N7C74_004280"
     CDS             2600..3571
                     /locus_tag="N7C74_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2434027.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            3568..4524
                     /locus_tag="N7C74_004281"
     CDS             3568..4524
                     /locus_tag="N7C74_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU2434028.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVLPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            4611..5660
                     /gene="yahK"
                     /locus_tag="N7C74_004282"
     CDS             4611..5660
                     /gene="yahK"
                     /locus_tag="N7C74_004282"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HGU2434029.1"
                     /translation="MKIKAVGSYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT         1281 a         1266 c         1607 g         1514 t
ORIGIN      
        1 tctgcataaa aatattcgaa aattcttatt ctctccagcg cgctaatttg ggcgaacact
       61 tcctgactac cctgcaatga ggctgaaatg atgaataaac gttttgttat caatatggtg
      121 tcttcgctgt tattaggcgc tgcgctaatt tctgcgccgc tccaggcagc ggaaaaagtc
      181 gtggtgaata tttctaaagt cgatggtatg ccgtggttta atcgcatggg cgaaggtgtg
      241 gttgaggcgg gtaaagcctt tggtgttaat gcctcacagg tggggccttc aagtactgat
      301 gctccgcaac aagttaaaat tattgaggat ttaatcgccc gtaaggttaa tgcaattact
      361 atcgtgccaa acgatgccaa cgtgctggaa ccggtattca aaaaagcgcg agatgccgga
      421 attgtggttc tgaccaacga atcccccggt cagccaagtg ctaactggga tatcgaaatc
      481 attgataacg aaaaatttgc cgctgaatat gttgagcata tggcgaaacg catgggtggc
      541 aagggcggct atgtgattta cgtgggtagt ttgaccgtgc cgcagcataa cttatgggct
      601 gacttgctgg tgaaatacca gaaagagcat tacccggata tgcatgaagt tacccgccgc
      661 atgccggttg ccgagagtgt ggatgtctca cgtcgtacta cactggacct gatgaaaacc
      721 tatcctgact tgaaagcggt tgtctccttt ggttcaaacg gtccgattgg cgcgggacgt
      781 gcggtgaaag agaaacgggc gaaaaataaa gtcgcagtct atggcatgat gatcccttca
      841 caggcggcat cactgattaa aagtggtgat atcaccgaag ggattactta cgatccggcc
      901 tctgcaggtt acgcactcgc agccgtcgcc agcacgttgc tgaagggtga agagatcaag
      961 cctggattag aaatgcagaa tctgggtaag gccgacgtag atatggataa gcgaattatc
     1021 cgtttccata aggtattact ggtcaacaaa gacaatatcg actcacttta ttaatccatt
     1081 tcttcggggc gaaagccccg attcttattg ccaggggatg ttatggaaac cttcctttcc
     1141 cttcgtcata tcaacaaaac gtttcatgcc acgcgagcgc tgcgcgatgt ttctctcgac
     1201 tttatgtcgg gtgaagtgca ctgccttgcc gggcaaaacg gctgtggtaa atcgacgttg
     1261 attaaaatta tgtccggtgt ttatcgcccg gatgagggag cggaaattac gcttggtggg
     1321 aaaaactggt caaagctgac acccgccgct tcggtggcgc aggggattca ggtgatttat
     1381 caggacctct ctttatttcc taacctgagt gtctgggaga atatcgccgt gaatcactat
     1441 caccacggtc tgtttgttaa ccgccgtcgt ctgcgtgagg tggcgcaggc ggcaatgact
     1501 agtattaacg tcacattgcc actggatacg ctcgtttctg agctttcaat tgcccgctgt
     1561 cagctggtgg caatttgccg tgcactggcg caggacgcac ggttgatcgt gatggatgaa
     1621 ccaaccgctt cgctgaccca tcaggaagtg caagggttat tacaggtggt gcatcaattg
     1681 cgtgaacgcg ggatctgcgt ggtctttgtc agtcatcgtc tggaagaagt gatggaagtt
     1741 tccgaccgta tttcagtgct gaaagatggt gagctggtcg ggacttttcc ggctgcagag
     1801 atgaccacaa aacaactcgg tttcctgatg accggccagg agtttgaata tcaggtgcga
     1861 gagttgtggc agggaaaatc cagcaccccg gtgctggagg tgcgtaattt aagtcgtcat
     1921 ggggaatatc taaatattaa cttgcgggtg gaagcgggcg aagtggtatc gattgttggc
     1981 ctgcttggtg cggggcgcac ggaattatgt ctgagtctgt ttggtatgac ccgaccagat
     2041 gctggcgaga tcctcatcaa cggccagctg gttacactgc atagtaacca ggacgccatc
     2101 cgccacggta ttggttatgt ttcggaagat cgcatgtcgc gcggtttggt gatggcgcaa
     2161 tctattgaag acaacatcat tagcactgtt tttcacaagg taaaagatcg cttcggcttt
     2221 ctgagtgaaa ctaaagtgcg tgacctggtg gacaggctaa tcaaggcatt gaccatcaaa
     2281 gtctctgatc ctcatcttcc ggtgaatacg ctttctggtg gtaacgcgca gcgagtatcg
     2341 atcgccaaat ggctggcgat tgggccacga ctgttgattc tcgatagccc gacagtcggt
     2401 gttgatatcg ccaacaaagc aggaatatac ggcattatca gcgatctggc ggctcatggc
     2461 attgcggtat tgatgatctg cgatgaaatt gaagaagcgt ggtatcaaag ccaccggatt
     2521 ttggtaatgc aaaaaggtca gatcacccat agcttcctgc ctgacagcag ttctcaggcc
     2581 aggattgcgg aggtggtaaa tggctgaact gaaaaaacgc catgaattct ggctggcatt
     2641 actgattgtg gtgctgtttg ttggccttgc ctggcgcagc gacgagtttc tgacattcgg
     2701 taatttgtac gatctcgcca ataactatgc catgttgact attctcgcct gtggtttgtt
     2761 tgtggtgttg atttccggtg gaattgatat ttcgtttcca gcaatgacca tcattgcgca
     2821 atacggcatg gtgctgttgc tgcaaaaaat tggtggcaac ttcgctgtcg cgtttgcact
     2881 ggcaggttgc atcggcattt tacttggctt aattaacgcc ttactggtta atcgcctacg
     2941 ggtgccttct atcatcatca ctatctcgac gctgaatatt ttctatggcc tgctgttatg
     3001 gttgagtaaa ggtgtgtggc tgtacgactt tccgccgtgg tttgagcagg gggttatgtt
     3061 gttcaagtac accgatgctg atggctatga ctatggcctt ggtctgccgc tgatcgccat
     3121 gattacggtg gtgctgctga cagcgtttat catgaatttc accagtgtag ggcgcaaaat
     3181 ttatgccctt ggcgggaacc gcgaatcagc cagtcgcatc ggctttagcg tgctgaaact
     3241 gcaacttttc gtctacggct atatgggatt gatgtctggc gctgcgggtg tagtgcagtc
     3301 gtggacggtg atgactgtcg cccccgattc tcttctgggt tatgagctga cagtactggc
     3361 tgcggtggtg cttggcggca ctagtttgct cggcgggcgc ggcacgttaa ctggtacttt
     3421 gctcggcgtg gtgttgttgg cagtgatgca aaacgggcta aatttattgg gagtctcgtc
     3481 ttactggcaa acattgatca ccggcatcat catcgttgcc agcattagcg ccacggcgtg
     3541 gagtcagcat cagaaccgga gcctgctatg aaaaaatcgt ggcgtaataa cgttgagttt
     3601 tacttgatcg gcctgctggt gctaactgtt gccgcgttca gcattactat gccggagatt
     3661 ttctggtcga taagtaattt ccagtccgtc gcttcgcaaa tgcccgtgct gggcattctg
     3721 gcgttggcta tggcggtgac catgctttgc ggtggcatta acttgtcaat tatagccact
     3781 gctaacgcct gttcactggt gatggcgtgg gtggcgactc agtatccacc gggtattgct
     3841 acggttgtcg ctacgttact ggcgggcgct ggcgcggcgg tgataatcgg tttatgtaac
     3901 ggcgtattga ttgctggtat tcgagtgttg ccaattcttg caacgcttgg gatgatgacg
     3961 ttgctcaaag ggataaatat tctggtgaca ggtggtagcg ccattgccaa ttatccctca
     4021 tgggtgctgt ggttgaatca tgcgcaatgg ttcggtattc cgctaccgat gtggttgttt
     4081 gccgcagtgg cactggggct gtggatattg ctggagaaaa cgccgctcgg taaagccatt
     4141 tacctgattg ggtctaacga acgagcaaca ctttactccg gcattaacac tcgtcgggtg
     4201 ctgatttggg tttatgtcat ttccgccttg ctttgtgcgg tagcggcatt tttgatgatg
     4261 tcgaaactca actcggctaa ggcctcttat ggcgaatcgt acttgctggt gtcaatcctc
     4321 gcggcagtac tcggcggggt taatccggac ggcggcagcg ggcggataat tggtatggtg
     4381 ctggcgctgt ttcttctgca aattattgaa agcggcttca atattctcgg tataagcccc
     4441 tacctgacga tggccttatg gggaacgttg ctgctctgtt tcattcaggc tcgcggcatg
     4501 ttggggctgg atcgggtggt ttaaatattg gctatattca atggacgcgt tttgccgcga
     4561 tgacatatca ggcgtcgcca aatacacata gctaatcagg agtaaacaca atgaagatca
     4621 aagctgttgg ttcatattcc gctaaacaac cacttgaacc gatggatatc acccggcgtg
     4681 aaccgggacc gaatgatgtc aaaatcgaaa tcgcttactg tggcgtctgc cattccgatc
     4741 tccaccaggt ccgttccgag tgggcgggga cggtttaccc ctgcgtgccg ggtcatgaaa
     4801 ttgtggggcg tgtggtagcc gttggtgatc aggtagaaaa acatgcgccg ggcgatttgg
     4861 tcggtgtcgg ctgcattgtc gacagttgta aacattgcga agagtgtgaa gacgggctgg
     4921 aaaactactg tgatcacatg accggcacct ataactcgcc gacgccggac gaaccgggcc
     4981 atactctggg cggctactca caacagatcg tcgttcatga gcgatatgtt ctgcgtattc
     5041 gtcacccgca agagcagctg gcggcggtgg ctcctttgtt gtgtgcaggg atcaccacgt
     5101 attcgccgct acgtcactgg caggccgggc cgggtaaaaa agtgggcgtg gtcggcatcg
     5161 gcggtctggg acatatgggg attaagctgg cccacgcgat gggggcacat gtggtggcat
     5221 ttaccacttc tgaggcaaaa cgcgaagcgg caaaagccct gggggccgat gaagttgtta
     5281 actcacgcaa tgccgatgag atggcggctc atctgaagag tttcgatttc attttgaata
     5341 cagtagctgc gccacataat ctcgacgatt ttaccacctt gctgaagcgt gatggcacca
     5401 tgacgctggt tggtgcgcct gcgacaccgc ataaatcgcc ggaagttttc aacctgatca
     5461 tgaaacgccg tgcgatagcc ggttctatga ttggcggcat tccagaaact caggagatgc
     5521 tcgatttttg cgccgaacat ggcatcgtgg ctgatataga gatgattcgg gccgatcaaa
     5581 ttaatgaagc ctatgagcga atgctgcgtg gtgatgtgaa atatcgtttt gttatcgata
     5641 atcgcacact aacagactga aaaaatta
//
LOCUS       DBFIFM010000247         5603 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.247, whole genome shotgun sequence.
ACCESSION   DBFIFM010000247 DBFIFM010000000
VERSION     DBFIFM010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.247"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..197
                     /locus_tag="N7C74_004283"
     CDS             <1..197
                     /locus_tag="N7C74_004283"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434030.1"
                     /translation="FFSPKEQGWGTFKLLCFSKLGSSPFDEKTITLSCFFESLFQLIF
                     RDFLLRQVIGFAAIIFADIP"
     gene            254..4066
                     /locus_tag="N7C74_004284"
     CDS             254..4066
                     /locus_tag="N7C74_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HGU2434031.1"
                     /translation="MAMINLSKEATVGKALTPIAILMMLSFPVASQAAGLVIKNGTVY
                     NANGVPVVDINKPNGSGLSHNIWDNLNVDKNGVVFNHSANESSTSLAGNIQGNSNLTS
                     GSAKVILNEVTSKNPSTINGMMEVAGDKADLIIANPNGITVNGGGSINTGKLTLTTGT
                     PDIQDDKLAGYSVNGGTITLGKLDNASPTEILSRNVVVNGKVSADELNVVAGNNYVNA
                     AGQVTGSVSATGSRNGYSVDVAKLGGMYANKISLVSTEKGVGVRNLGVIAGGVNGVSI
                     DSKGNLLNSNAQIQSASTINLTTNGTLDNTTGTVTSVGTISLNTNKNTIVNTRAGNIS
                     TMGDIYVNSGTIDNTNGKLAAAGMLAVDTNNATLINSGKGSSVGIEAGLVALKTGTLN
                     NSNGQIRGGYVGLESAALNNNNGDIQTTGDIAIISNGNVDNNKGLIRSSTGHIVIGAA
                     GSVNNGSTKTADTGSSDSLGIIADTGVEIGANNINNNGGQIASNGNVSLSSYSTIDDY
                     AGKILSNSKVIIKGSSLRNDTGGISGKQGIEVAVGGSLTNNIGVISSEEGDISLLANS
                     VDNHGGFMMGQNITMESMSGVNNNTALIVASKKLKINARGSIENRDGNNFGNAYGLYF
                     GMPQQTGGMVGKEGIELSGQNIYNNNSRLIAEDGPLTLQAQNTFDNTRALVTSGADAS
                     IQVGGTYYNNYATTWSAGNLDIDATTLQNSSSGTMIDNNATGFIASDKNLSLEVVNSL
                     TNYGWISGKGDVDVTVNNGNLYNRNTIAAEKGLDIAALNGIENWKDISAGGDLTMNTN
                     RHVTNNSNSNMVGQNIVINAVNDINNRGNIVSDADLNVTTKGNLYNYLYMVGYGDIAL
                     SANSVANNNATIEATGDLIIDSKGNVGNNRGNLHALNGVLSVKGNNLNNDNGEIRGYG
                     DVTLALTGNYDSYKGSLTSETGDVTLTANIVDNAYGLIAGENVSVDAKSTIYNNTALI
                     AANKKLVINAGGNLENRDGNNFLRNNGALFGITDNVGGIVGKEGVTLSAQNVYNNNSS
                     IIAENGPLNLLSRGTLDNTRALLSSGADAIIRAAGTFYNNYATTYSAGNLDVYAASLN
                     NASDGRLEDNTATGVIASDKNLDLSVDNSVTNYGWISGKGDVHFNVLKGTLYNRNAIA
                     ADNALTINALNGVENFKDIVAGTALTIDTQKYVTNNSNSNMLGQTIAINAVNDINNRG
                     NIVGDYSLGVKTTGNIYNYLNMLSYGVAGVSANKVTNSGKDAVLGGFYGLALEANETD
                     NTGTIVGM"
     gene            4155..>5603
                     /locus_tag="N7C74_004285"
     CDS             4155..>5603
                     /locus_tag="N7C74_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HGU2434032.1"
                     /translation="MQLRFNFGLLLILCCFQVAAKDSLDNLIKQQKNADESTLRAHKL
                     EKKDVYSNAQRNAFTLGDLPQEKNCFIINRIELEDNFLGGQLISEVQKAIAGRCVGSA
                     GVQKIAAALQDYYINAGYITTRISIPSQDISKGKLRFQVNAGKIEKTIVEGNDISEWM
                     LPFQRNDILNIRDIEQGLENLQRVPDVDVKINIEPGTRDGYSIVHIDTHRGKNWSVRA
                     SYNNWGDEETGRYQTTSVGYLFNPAKMGDLFYLAGTRSTTGQYENVSSYYSIPVGYWE
                     YGFFYSKSKSQQVIPLSYISLDYVGISEYLSAKATHTVYRDKTKKFAGSAELIRRKSN
                     YTLNGEELTLQARDMGNIKLGLNYKQQLPGAFWDSTLSWQRFLTWFGGEKTPDMQYGD
                     VSPVSNIFNFEGNYTRQIENGYYNTAFFAQYAPRELTLQDKITVGDRWSIRGFENSVG
                     LSGNDGFYIKNTLAFPLPGMKANYYAGLDFGQV"
BASE COUNT         1580 a         1178 c         1448 g         1397 t
ORIGIN      
        1 gatttttttc acccaaggag caaggatggg ggacttttaa attattgtgt tttagtaagt
       61 taggttcttc gccgttcgat gaaaaaacaa taactcttag ttgttttttt gagtccttat
      121 ttcagcttat ttttcgtgat tttttattgc gccaggtgat aggatttgcc gcaatcattt
      181 ttgctgatat tccgtgagtc atcaaaagtg atctgcatgt ccaaaatgaa tagcatcaat
      241 aaggaaaacg ttagtggcga tgataaattt aagtaaggaa gcaacggtgg ggaaagcatt
      301 aacccctatt gctatactta tgatgttgtc ttttcctgta gcttctcaag cggcgggatt
      361 agtcataaaa aatggaacgg tatataacgc caatggtgtg ccagtcgttg acatcaacaa
      421 acctaacggt agcggtttat ctcataatat ctgggataac ctaaacgttg ataaaaatgg
      481 tgtcgttttc aatcatagcg ctaatgaatc cagtacttca cttgccggaa atattcaggg
      541 aaacagtaat ctgacctccg ggtcggcgaa ggtgatcctg aatgaggtta cttccaaaaa
      601 tccttcaacc attaatggga tgatggaagt tgcaggggat aaagcggatc tgattattgc
      661 caacccgaat ggtattactg taaacggtgg cggttcaatc aatacaggta aacttacctt
      721 aaccaccggg acgccggata tccaggatga caagctggcc ggttactccg tgaacggcgg
      781 taccattacg ctcggtaaac tggataacgc cagcccgaca gaaattctgt cccgtaacgt
      841 ggtagttaac ggcaaagtgt ctgccgatga gctgaacgtt gttgctggca ataactatgt
      901 taatgccgca ggccaggtga ccggtagcgt atccgccacg gggtcccgta acggttacag
      961 cgtagatgtt gccaaactgg gcggaatgta tgcgaacaaa atcagtctgg tcagcaccga
     1021 gaaaggtgtg ggggttcgca acctcggcgt tattgctggg ggtgttaatg gtgtcagcat
     1081 cgattccaaa ggtaacctgt taaacagtaa cgcccagatt cagtctgcaa gcacgatcaa
     1141 cctgacaaca aatggtactc tggataacac caccggtacg gtgacatctg taggcactat
     1201 ctcgcttaat accaacaaga atactatcgt gaatacccgt gcgggtaaca tctctacgat
     1261 gggcgatatc tacgttaaca gcggtacgat tgacaatact aacggcaagc ttgcggctgc
     1321 aggaatgctg gcggttgata ccaataacgc cacgctgatt aactctggta aagggagttc
     1381 tgtcgggatt gaagcggggc tcgtggcgct gaaaaccgga acgctcaaca acagcaatgg
     1441 tcagattcgc ggtggctatg tgggtcttga atccgctgcg ctgaataaca acaacggtga
     1501 tatccagacc accggcgata tcgccattat cagtaacggt aatgtggata acaacaaagg
     1561 tctgatccgt tcgtccaccg ggcatatcgt tattggcgcg gcaggtagcg taaataatgg
     1621 ttcaaccaaa accgccgata ccggcagttc tgactctctg ggcattattg cagataccgg
     1681 cgtagaaatt ggtgcgaaca acatcaataa caacggcgga cagattgcgt ctaatggcaa
     1741 cgtctccctg tcaagttaca gcacgatcga cgactatgcg ggcaaaattc tgtccaacag
     1801 caaagtgatt atcaagggaa gctctctgcg taacgatacc ggggggatca gcggtaagca
     1861 gggtattgaa gtcgccgttg gcggcagcct gaccaataat attggcgtga tcagctctga
     1921 agagggtgat atctccctgt tagccaactc cgtggataac cacggcggct tcatgatggg
     1981 gcagaacatc acgatggagt cgatgtctgg cgtcaataac aacacagcgc tgatcgtggc
     2041 cagcaaaaaa ctgaagataa atgcgcgcgg cagtatcgaa aaccgcgatg gcaataactt
     2101 cggtaatgct tatggtctgt acttcggcat gcctcagcaa acgggtggaa tggtcggcaa
     2161 ggaaggcatc gagctttccg ggcagaacat ctataacaac aacagccgtc ttatcgctga
     2221 ggatggtcct ctgactctgc aggcgcagaa cacgttcgac aacacgcgtg ctctggtcac
     2281 cagcggggcg gatgcatcta ttcaggttgg cggaacgtat tataacaact acgctaccac
     2341 ctggagtgcg ggcaacctgg atatcgacgc gaccacgctg caaaacagca gcagcggtac
     2401 gatgatcgat aacaatgcga ccgggttcat agcatctgat aaaaacctgt cactggaagt
     2461 ggtgaatagc cttaccaact acggctggat cagcggtaaa ggcgatgttg atgtcacggt
     2521 gaataacggc aacctgtata accgcaatac cattgcggct gaaaaggggc tggatattgc
     2581 cgcgttgaac ggtattgaaa actggaagga tatttctgct ggcggcgacc tgacgatgaa
     2641 caccaatcgc catgtgacca acaactccaa cagcaatatg gtggggcaga atattgttat
     2701 taacgcggtt aacgatatca acaaccgtgg caacattgtc agtgacgctg acctgaacgt
     2761 gacgaccaaa ggcaacctgt ataactatct ctatatggta gggtatgggg atatcgcatt
     2821 gtcggcaaat agcgtggcga acaataacgc gaccatcgaa gcgacaggcg atctgattat
     2881 cgattcgaag ggtaacgtgg gtaacaaccg cggtaatctg catgcgttga acggcgtgtt
     2941 gtctgttaaa ggcaacaatc tgaacaacga taacggtgaa attcgtggtt atggcgatgt
     3001 cacgctggca ctgacgggca actacgacag ctataagggt tcgctgacct ctgaaacggg
     3061 cgacgtgact ctgacggcga acattgtaga caacgcctat ggtttgattg ccggtgagaa
     3121 tgtttctgtc gatgctaaat cgacgattta caacaacact gcgctgatcg cggcgaataa
     3181 aaagctggtt attaacgctg gcggcaacct cgaaaaccgc gacgggaata acttcctgcg
     3241 taataacggc gcgctgtttg gaattaccga caacgttggc ggcatcgtag gtaaagaagg
     3301 tgtcacgctt tctgctcaga acgtctacaa caataacagc agcatcatcg ctgaaaatgg
     3361 tccgcttaat ctgctgtcca ggggaacgct ggataatacc cgcgcgcttc ttagcagtgg
     3421 ggctgatgcc atcatccgtg cggcagggac gttctacaac aactatgcca ccacgtacag
     3481 cgccggtaat ctcgacgttt atgcggcgtc gttgaacaac gccagcgatg gtcgcctgga
     3541 agacaatacc gccacgggcg tgattgcgtc tgacaaaaac ctggatctga gcgttgataa
     3601 cagtgtcact aactatggtt ggatcagcgg taaaggagat gtgcatttca atgttctgaa
     3661 aggcacgctg tataaccgta atgccatcgc ggcggacaac gcgctgacca ttaatgccct
     3721 gaacggtgtt gagaacttta aagacattgt ggcgggtact gcgctgacta ttgatacgca
     3781 gaagtatgtt accaacaaca gcaacagtaa tatgttggga caaaccatcg cgatcaatgc
     3841 cgtgaatgac attaataacc gtggaaatat tgtgggtgat tattctctgg gtgttaaaac
     3901 caccggtaat atttataact acctcaatat gctgagttat ggtgtcgctg gcgtatcggc
     3961 aaataaggtt acgaatagcg gtaaagacgc tgttctcggt ggcttctacg gtttagcgtt
     4021 agaagcaaac gaaactgata acaccggtac tattgtcggc atgtaagatg taaccaaaga
     4081 cagcagtggc ttttaatatt aatgcgtcat gtcacgcagg ggttgctgct gtctttattt
     4141 agtttgagaa gataatgcaa ttacgattta attttggttt gctgctcatt ctttgttgtt
     4201 ttcaggttgc agcgaaagac agtcttgata atttgatcaa gcagcaaaaa aatgcagatg
     4261 aaagcacgtt gcgcgcgcat aaattagaaa aaaaagatgt ttactcaaat gcacaacgta
     4321 atgcgtttac tctgggtgat ctgccgcagg aaaagaattg ctttatcatt aatcggattg
     4381 aactggaaga taactttctg ggtggtcagt tgatttctga agtgcaaaaa gcgattgccg
     4441 gacgttgtgt cggtagtgct ggtgtgcaaa aaattgcagc tgccttgcag gattattata
     4501 ttaatgcggg atatatcaca acgcgaatta gtatcccttc gcaggatatt tctaaaggca
     4561 agctacgttt ccaggttaat gccgggaaga tagaaaaaac gattgtcgaa ggtaatgata
     4621 tcagtgaatg gatgttacct tttcagcgta acgatattct caatattcgt gatattgaac
     4681 aggggctgga aaatcttcag cgagtacctg acgttgatgt gaaaattaat attgaaccgg
     4741 gaacacggga tggttacagc atcgttcata ttgatacaca ccgggggaaa aactggagcg
     4801 ttcgggcaag ttacaacaat tggggcgatg aagaaacggg ccgttaccag acaacctcgg
     4861 tgggttatct gtttaatcca gcgaaaatgg gggatctgtt ttatctcgcc ggtacgcgta
     4921 gtactaccgg acagtatgaa aacgtcagta gttattattc cattccggtg gggtactggg
     4981 agtacggctt tttctatagc aaaagtaaat cgcagcaggt gattccgctt tcgtacatct
     5041 cgctggatta tgttggcatc agcgaatact tgagcgcaaa agcgacgcat accgtttatc
     5101 gcgataaaac aaaaaagttc gccggtagtg cagaactcat tcgacgaaag tcgaattaca
     5161 cgcttaatgg tgaggagctg acattacagg cgcgggatat gggaaatatc aagctggggc
     5221 taaattataa gcaacagtta cccggcgcgt tctgggattc aaccctctcg tggcaacgct
     5281 ttttaacctg gttcggtggt gaaaaaacgc cggatatgca atatggcgac gttagcccgg
     5341 taagcaatat ctttaatttt gagggtaact atacccgaca aatagagaat ggatattaca
     5401 ataccgcttt ttttgcccag tacgcaccgc gtgaactgac tttgcaagat aaaattacag
     5461 ttggcgatcg ttggagtatt cgcgggtttg aaaatagcgt aggtttgtca gggaatgatg
     5521 gtttttatat aaaaaatacg ctggcatttc ctttgcctgg aatgaaagct aattattatg
     5581 ccggtcttga ttttgggcaa gtg
//
LOCUS       DBFIFM010000248         5499 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.248, whole genome shotgun sequence.
ACCESSION   DBFIFM010000248 DBFIFM010000000
VERSION     DBFIFM010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.248"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            70..258
                     /locus_tag="N7C74_004286"
     CDS             70..258
                     /locus_tag="N7C74_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2434033.1"
                     /translation="MLSTDRFIREKECEKLTGLSRTCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            316..870
                     /locus_tag="N7C74_004287"
                     /pseudo
     CDS             316..870
                     /locus_tag="N7C74_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            1063..1260
                     /locus_tag="N7C74_004288"
     CDS             1063..1260
                     /locus_tag="N7C74_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU2434034.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARN
                     RCPSGCVAVFAARIRQEVRHD"
     gene            1253..1465
                     /locus_tag="N7C74_004289"
     CDS             1253..1465
                     /locus_tag="N7C74_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434035.1"
                     /translation="MINLSLTDLNRIQFREKFTGQLLVNVDKGRVVCNYHLPDEAIVA
                     TRESLQEITKRAGMINTNIQGMLYAQ"
     gene            1455..1919
                     /locus_tag="N7C74_004290"
     CDS             1455..1919
                     /locus_tag="N7C74_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434036.1"
                     /translation="MHNKTTPDAAAEAIKTLMHALIDISVIADRAHKHATSETEYAGA
                     FVPHSLAVMQFSADTALNEAKAILIADCENGGVMRDDRFNSLKQEFSGVPDDAADALS
                     SMPELIRAAFFLLSTREYKSTGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            1912..2145
                     /locus_tag="N7C74_004291"
     CDS             1912..2145
                     /locus_tag="N7C74_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434037.1"
                     /translation="MRDIYHQTIDRAFLALSHSENMMEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            2151..2450
                     /locus_tag="N7C74_004292"
     CDS             2151..2450
                     /locus_tag="N7C74_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU2434038.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMVNGFRGKK"
     gene            2447..3847
                     /locus_tag="N7C74_004293"
     CDS             2447..3847
                     /locus_tag="N7C74_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HGU2434039.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELAELRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPAEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVIRIDGDDVAPL"
     gene            4048..4299
                     /locus_tag="N7C74_004294"
     CDS             4048..4299
                     /locus_tag="N7C74_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434040.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVEL"
     gene            4296..4706
                     /locus_tag="N7C74_004295"
     CDS             4296..4706
                     /locus_tag="N7C74_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU2434041.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQAGFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
     gene            4717..4989
                     /locus_tag="N7C74_004296"
     CDS             4717..4989
                     /locus_tag="N7C74_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU2434042.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            4946..5074
                     /locus_tag="N7C74_004297"
     CDS             4946..5074
                     /locus_tag="N7C74_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGU2434043.1"
                     /translation="MVLVMKYGAITRTANQTAGEIRLFLHQLKRYGALPGFRHGQA"
     gene            5116..5340
                     /locus_tag="N7C74_004298"
     CDS             5116..5340
                     /locus_tag="N7C74_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434044.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKEIRD"
BASE COUNT         1527 a         1254 c         1512 g         1206 t
ORIGIN      
        1 cactctagcg ggaacaatac gacacaataa gacacctgat gactctttaa gaaacgaaag
       61 ggggcaatag tgttaagcac tgatcggttt atacgtgaaa aagaatgcga aaaactaacc
      121 ggccttagcc gtacgtgtcg ctaccgcctg gaaaaggccg gacaattccc atcacgtcgt
      181 aaacttggcg gtcgttccgt tggctggtct ttatccgagg ttctggcctg gaaggatagc
      241 tgcaaggcag ttcattaatc acgctggcgg cacacagccg ccacacatca atcatctgaa
      301 cacagagcta taaccatgaa gattgaatat acgccagaac gtgggcgggg attcgttcgc
      361 cctggtgaga ctggaaaacc acaaaattgg ggtttttcag gcataaaaaa agcggccccg
      421 aaatggagcc gcctttctga acagataacc cgctgcgcct tatgtgtata tgatcccaaa
      481 cataagcacg gggatgatag ccgctatcag gctggtgggc aatgctatca gtctggttca
      541 gttcgttgcc atacctgcaa tgggcgcgtt tccctgtact ctttaaggaa ttgctcaagg
      601 gcaaaagcac atggcgcgaa tctttctgat tcatgctcta tctttctgcg ccgtcttttc
      661 cgtgccggtg ataatgtttt ggtcaattct ttatcggtca ttgtgttgtc ctgcatagca
      721 atgcgccgta atacctcaca ccacggcgct gatagttttt atcctttggg ttctatgccg
      781 cgttgctgta attctttgcg gagaacgcgt ttaatccaag tggctaaaga tgtgtcgcca
      841 tcggccttag cagcttcttc tagctgcgct ctgaagtctt ccggtaatcg catttggtat
      901 ggaggcgaac gtttttctaa tggtgttgac atggtaatta ccctttattg ctatacctac
      961 atggtaatta ccattgtact tgccattaca caaaaatcaa cgccccgcag tgctgggaac
     1021 acatacgggg cgtctaacca acaacgtaaa ctaggagccg ttatggttgc cgtaaatcat
     1081 ataccacacc ttgtacacac acaaacggcc tttgtgtggc gttttctggc actgagtgcc
     1141 ggagaatctc aaatcatcca cgtaaccgcc tggacggaac gcgaagcgcg taaccgttgc
     1201 ccgtccggtt gtgttgctgt atttgccgcc cgtattcgtc aggaggtgcg ccatgattaa
     1261 tctgtcactt actgacctta accgcattca gtttcgtgag aaattcacag ggcagctact
     1321 ggtcaatgtg gataaaggtc gcgtggtgtg taattaccac ttaccagatg aagcaattgt
     1381 cgccacaagg gaatcattac aggaaatcac aaaacgtgcc ggaatgatta acacgaatat
     1441 tcaggggatg ctttatgcac aataaaacca caccggacgc agccgccgaa gccatcaaaa
     1501 cgctgatgca cgcgctgatt gatatttctg ttatcgcgga cagggcacat aagcacgcca
     1561 ccagcgaaac agaatatgcc ggtgctttcg ttcctcactc actggctgtc atgcaattta
     1621 gtgctgatac ggcgctgaat gaggccaaag ctatcctgat tgctgattgt gaaaatgggg
     1681 gggttatgcg tgatgatcgt tttaattccc tgaaacagga attttccggc gttcctgatg
     1741 atgcggctga tgcgctttcg tcaatgccag aacttattag agcggctttt ttcttacttt
     1801 ccacgagaga atataaatca acggggcttg atgtactgaa tatcgccgcc gattatgcgg
     1861 aatatgtggc agaggcgcgt tatagaagaa aatttcctga ggatgtaagc catgcgtgat
     1921 atttaccacc aaacaataga ccgcgcattt cttgcacttt ctcacagtga aaacatgatg
     1981 gaaatattgc gcatatggct tgaaacactt ggcgacaatg aacgcgacaa acaaaaatca
     2041 agaattgcca cggcattaat aacgcttctt gagcctgtaa taatggaact gcaagaaata
     2101 gatctattgc acgacagata taaagaacag cacaccggag aataaaaata atgaaactta
     2161 aatattctgg cttaactgcc agtggcaaca ctcaccctaa atttacgcgc ggtgatattt
     2221 accgcgacca gtacggcggc acggtaatga ttaagggcgt ggaagaacgg cgcgtaacct
     2281 accgccgtga aggttacgaa tatgattgcg tgatgcctgt ttatcagttc cggcgtgatt
     2341 tttctctggt acagaccgcg ccgcataacg tgcccaccag caacgccagg gcacgggcaa
     2401 acatccagaa gctgaaaacc atggttaacg gattcagggg caagaaatga aaagcgcacc
     2461 gaacttaaaa aaacagcctt acgacaagat gaccgaagtc attatttttg cgggtagtga
     2521 tgcctgggca catgcgaaac agtggcagga acaggacggg cgactggctg gcgataatgt
     2581 gcctcccgtt gtgctggctg atgatcaact ggatgaactg gcagaactga gaatcatcga
     2641 cgaggggcgc tattgtgtcc ggctgtacaa ggcaggccac atcaggccat caaatattaa
     2701 tgccattgcg cacaagctgg cggcggcggg tgtaactgat gcgaattatt accccgaagg
     2761 gatgcacagc catatgcggg agaactggcg cgaatacctg gaacgggtgc gcgggaaaga
     2821 gccggcggaa gaaaaaaacc accagcgaaa aaccacgcta ccgatgagcg ttggatctac
     2881 cggatacgac acgcaactgg attacgtggt taaggggatt attccggcgg tatcgctatg
     2941 cagcatatac ggggctagcg ggtcctataa atcattcctt gccggatcgt gggcgtgcca
     3001 tgttgccact ggtcgccagt ggggaggccg cagggttgca catggtgcgg ttctctatgt
     3061 ggttggtgaa ggcggtatag gtgttccgcg tcgtgtaaaa gcctgggagg ttgtgcacga
     3121 tgagcaggtg aaaaatctgt atctggtaaa ccgccccatc tttccggctg ccccgcttga
     3181 tgttgatgaa atggttatcg ctgcccgtca ggtggagcgg gaaacgggta aacctgtacg
     3241 catgattatt ctggatacgc tggcgcgttg ctttggtggg aatgatgaaa atgattcccg
     3301 tgatatgggg gcgtttatcc gtggttgtga cgaactgaaa cgacgcacag gggccacggt
     3361 gctggtggtt caccattccg gcaaggatga gacgaaaggc gcgcgcggtt ccagtgcatt
     3421 tcgtgcttcg ctggatgctg aataccggat acgcagggag gacgcaggaa gcgaagcgct
     3481 ggttatctca tgcaccaaaa tgaaggacgc ggaggaactc aaagaagccg catatgactt
     3541 acgcgtggtg gagcttttta ccgacgctga cggtgaatta atcacgtcgc tggtggtggt
     3601 ggatgatccg cgccctcctg ttgaactgga gcgcatcgag gaggcaggga acaagacgga
     3661 aaaccatacc gcgctatggg ggtgcatccg ttcacgcaca cagaacggcg acaagtgcac
     3721 gatcccgctg ttacgtgatg acatgaaaaa gctggggtat gaaatgaaaa acttccggcg
     3781 ctggctgtac aagctggaaa aagatggggt tattcgtatc gatggggatg atgtagcgcc
     3841 gctataaaag tgaggagcaa aagcgagggg gatagaaaga gggccaaaat tagcccgctc
     3901 tccctcactt ttcgacctgt atacatcctc aaaagtgagg ggtaaaaaaa tacttatgaa
     3961 acacacacat agaaaaaccg aaaatcccaa ctgcgacgaa gtgagacgct tgaaaaagtg
     4021 aggcgaaaaa gtgagaggtt gcgagaaatg acccaaaaac gcagagacag aacagagcca
     4081 aaatataaag cgttagacat gactgagcac accttaaagg tggcaatcag aacgatagac
     4141 cgccacacgc gggaaggata cgcgaaggaa catcccgacc tgataagcgc attcatgacc
     4201 acggcggcgg caaactttgc cacgctgaca gaacgggaga ttgccgaagc ggaacaggta
     4261 acaaccatca acgttaaaac cggagaggtg gaattatgac ggcacagata gccgcttacg
     4321 ggcggctggt ggacgacccg caggtaaaac agaccagcaa gggcacaccg atgacgctgg
     4381 cgcgtatggc ggtatctttg ccatgcagcc aggcacagga cggacaggcg acgttatggt
     4441 tatcggtcat cgcatttggc aaacaggccg gcttcctggc taaacatcaa aaaggcgatg
     4501 ttgccagcgt atccggcacg atgcaggtaa gccagtggac cggacagaac ggagaaacgc
     4561 ggcagggctg gcaggttatc gcagacagcg taatcagtgc ccgcgcggca cgtccaggcg
     4621 ggaacagacg caaaaccaca ggcacacagg gtaatcagcc accagcggga ggcgatgacc
     4681 cttacggtga tgatatttcg ttctgagggg gtgacgatgg tacatgaccg catagcggag
     4741 gaactggagg cgaaaggctt ttaccggagg gcggcggcgc gatggggtga agtcatgcag
     4801 ctggtggaga cagacaagga acggcatcac atcacgatgc gacggctgga atgttcaagg
     4861 aaggcacaga gggcaccgga gccgccggat aatttcggag acctgaaaaa ggcagtcgat
     4921 cgcacttatg ccgaaatggg tatagatggt gctggtgatg aaatatggcg caattaccag
     4981 gacagctaat caaacagccg gagaaatccg gctttttttg caccagttga aacggtatgg
     5041 cgcattaccg ggttttcgtc acggtcaggc atagttacta tctgaaacaa acagacacaa
     5101 cagaggaaaa aaacaatgcc gatgaaattt gatgagatat taaaacagcg tgataaatac
     5161 catgctgaca acatggagac gatgagcatc aatgattacc gcgcattcct ggagacgggc
     5221 gcactgattg aaaaggatca gcatggtttt gtgagatgtg ctctatccgg tgaaatgctg
     5281 gcggtaaatc ctgaacagat agatgcattg atagaatttc tgaaagagat cagagactga
     5341 gccagcacac agcacacata gccggagcaa tccggctttt ttgcgcccaa aaaaagcccg
     5401 ataaggtcag agggttctta tcgggctttt gcatatgagg tttttggatg cactggcgtt
     5461 cgtgatcggg ataatcattt cataatttgc aacataact
//
LOCUS       DBFIFM010000249         5424 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.249, whole genome shotgun sequence.
ACCESSION   DBFIFM010000249 DBFIFM010000000
VERSION     DBFIFM010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.249"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..585)
                     /gene="dosC"
                     /locus_tag="N7C74_004299"
     CDS             complement(<1..585)
                     /gene="dosC"
                     /locus_tag="N7C74_004299"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HGU2434045.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEID"
     gene            complement(962..2281)
                     /locus_tag="N7C74_004300"
     CDS             complement(962..2281)
                     /locus_tag="N7C74_004300"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HGU2434046.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(2412..3947)
                     /gene="gadC"
                     /locus_tag="N7C74_004301"
     CDS             complement(2412..3947)
                     /gene="gadC"
                     /locus_tag="N7C74_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HGU2434047.1"
                     /translation="MATLVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(4103..>5424)
                     /locus_tag="N7C74_004302"
     CDS             complement(4103..>5424)
                     /locus_tag="N7C74_004302"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HGU2434048.1"
                     /translation="ESKRFPLHEMRDDVAFQIINDELYLDGNARQNLATFCQTWDDEN
                     VHKLMDLSINKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEA
                     CMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQL
                     FMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAA
                     SGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNV
                     DYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLG
                     PYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIV
                     VMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
BASE COUNT         1401 a         1348 c         1335 g         1340 t
ORIGIN      
        1 atctatttcc catgaaagta ttgaggctat ttgccgttct ttttcttctt cggcgttttc
       61 cagtaacgag aagatacgat agttttcatc ttcttttgag gcactactgt cactaaaggt
      121 aaacgcgcgg gtcatcactt ccatagcgat atcaatactg ttaatcgaga aatggtagac
      181 ctgaagtttt tctgcggcag aataatccga agagaagatt accggataga ggatcttttt
      241 cagcacccga aaccccatct cgacaatttc taccggaatt cctatgcggg catgcacttc
      301 cgcgacggta tgctggattt gtattagcct ttcgacatcg tcaacctggg cagaaagcac
      361 gttaataatc cagcgttcca tcgcactttt caactgccgc tcaacttgtt cattactcaa
      421 gaattcttcg gcatgcgggt cgatgcggac aattcgataa aactcgatac tcagataatg
      481 agcatgcgca acggcaattt ccgcggcttt agcacgaatg ggcggatctg cctgctcgac
      541 aagtccggtc cactcatctt tcattctttt aaaatacatc tccataattc acacccttat
      601 aaggctggga aatcagacgg aatcaaaatg aaacgcgacg tgcgagatcg actaactgca
      661 ccatattctc ctgaaatatg aagatatact gaaaagaaat aagcgactta ggacagtttc
      721 aatctacgct actgttcttc agaagagtat agcccatcgt aattattttt cggtgactgc
      781 gaatatcgta tggtttttca tattcataca tttttattag gggatttatg gctgtttaac
      841 taagcgtggt taattgcgtg gttaatttga ctttagtaag catgattgct ggtgggatag
      901 ttcaagaggg taacaagcca gtgggtaaag caccggcttg ttacaaagta agaatgggag
      961 tttaactgcc ccaacgactt tgcagatagc tgaccgcttg ttgagtctgc ggtttattca
     1021 gatagtcctc acggaacaag atggtgccgc taatttctgg taccgcatcg ttaagatcga
     1081 gctgcttttt cagctccggt acgccgccgt taatcatcca gtctggctct atctttgaag
     1141 gttcacccac tttatagaag gcgataccga tatacaggcg ggtcctggtc ggtttaacga
     1201 catccgccca ccattttgcc aacacgtcat aacgcgcggc actccgtgag aacggccagt
     1261 aaatttgggg agcaatgtaa tccagcaatc cttgttccac ccatcgacga gtgtcagcgt
     1321 aggattcgtc ataggctgcc gcgcctcggg tatcggaacc gagcggatcg tgtgatcggt
     1381 tacgccacac gcctgccggg ctaacaccaa attcgactcc cggcttaatg cttttaatgg
     1441 tgtgcgatac ctttgcaatt aactgctgag tattgttgcg ccgccagtct gcttttgacg
     1501 caaatgcgcc tccgtattta cggtacgttt cgttatcatt tagccgtgaa cccggtgact
     1561 cagtatagaa atagtcgtca aactgcacgc catctaccgg atagcgggaa accacttctg
     1621 cgactattga tgtgatccag tcctgaacct cagggatgcc cgggtcgagg acaaagcgat
     1681 cgccagacgt tctgatccag tcacggtgtt gcacatagac gctcgccggt tgttgagaca
     1741 gagtgctatt cagttccctg atagtaccgg gcttcgtatt aaccgatacg cgataggggt
     1801 taaaccaggc gtgtactttc atcccacgct tgtgggcttc gtcgagcata aattgcagcg
     1861 gatcgtaacc cggattttca ccaatcttac cggtcataag atcggaccac ggcaaaattt
     1921 tcgatggcca cagggcggta ccgtccggct tgacctggaa aaagaccgtg tttataccga
     1981 gacgttgcaa atgatccagt ttgtcgatca tcgcctgttg ttgtacacgg gcccggctgg
     2041 tggggttact aatgttaacc gaggaaaccg gtggccagtc gaggcgagac accgtggcca
     2101 gccagatgcc acgcatcggt tgtgacgatt gttgcgtcgt ggctggtggc tttgaacccg
     2161 ctggtggtgt caccatggac tctggaggcg tgcttttaca actacacagt aaaagtgcca
     2221 gtgcaaccag tatcgctggt cttctaatcg ttaatttctt gtttcgggag cagatatcca
     2281 tatgtgctag tttccggtaa acagatgtgc gctcattctc gtacttatcc cggttaagtc
     2341 aatacgacag caagcacgaa aaagggagcg atgaattatc gctcccttat cttataacca
     2401 ttcagacata gttagtgttt cttgtcattc atcacaatat agtgtggtga acgtgcacgc
     2461 gggtgcagga agaagtgacc ttttggtgcg ttctgactgt tgattggctc cagagtgacg
     2521 ccggtattcg ctttgccttt acgatcatga acagcataga gaataaaggg cagggcaagt
     2581 accaccagga aactaacgac cagtaattca acatacatat cggtagagtc accctggatg
     2641 ttatccggcg gcaggaagga aacaataaac gccataattg aagtcagcag accgacaatt
     2701 gccacgacca gtttcacccc tttaccacca gggatattaa atgtgcgttt taagtcagga
     2761 tgtttaagaa ccaacacaat gtagccaata aacagcatga aataagcaca cagataaatc
     2821 accaccgtca gcgccagtgc gatcaggaag gacatgttgt taccgccacc ggtattggtg
     2881 aggatgatca acgcgataga agtaatcacc agctgcgaaa tgaccagcgt taccggtacg
     2941 ccatttttgt tcattttagc gaatgccgct ggcagcaggt ttttctgcgc tgtgacatac
     3001 atcccgcgag aaggaccaac aatccaggag gcgatttccg ccagaacacc cagcaacagc
     3061 agtgcggaga tcacgcgaac cgtccactca atttccggtg ccacatggga catcagaacg
     3121 gtaaaggttt gcattacccc tgcggagagg ttgatttcat tacccggaat gaccatcgca
     3181 atagacaaac cgccaacaga gcttaagcag attgccgcca ccatcagcag taacatagcc
     3241 agtggatagt cgcgccctgg gttactcatt tcattgacgt gggttgcgga agcttctacg
     3301 cccatataac tcaaaatgaa ggcaacaaat acaaccaggg tgcccacttt agagaagtca
     3361 gggaagaagg tcttcgaatc catttcgata gcaacggggg caccggagtg cagataaata
     3421 gccgctaatg caatcaaaat aaatgcaggt aacaggatac cggcgaagaa gccaacttta
     3481 gcaattcgcg ccgtgtattt cgtgccacca aactgcgtta atgccagcgc ccaaagaatg
     3541 atgagtgctg caatagtttt ggtaatgggg tcttcattca gcgctggcca tttcaggatg
     3601 taggagagtg cccctaacac gaaatagagc atcggaataa aaccaatggc gatttgcaga
     3661 tagccaaatg agatcgctgc aaatccccat ctcggcccca gagtatttga tacccaggcg
     3721 aagacaccac cttcttccca gccgtcgacg gtggccattt ccgcagcaca aagtcccacg
     3781 ggaataaacc ataaaatccc gcctaatagc aggaagaaga ctaatgaaaa gcccgatgtt
     3841 gcgaaggtag ggtattcata aacagccatt accatcgatg ccgttatggc aaaaaagccg
     3901 agtaatgtga gctgcttagc tttacctgtc tgtactaatg tagccatatt atccccctaa
     3961 aacggtattc ctgtcggaac cgcacctgtt ttgttctgaa gcgtattcag aacaatattt
     4021 tccgttgcta atgccagtga acagactttg gaaattgtcc cgaaacgggt tcgtttcggg
     4081 acaccgttac cgttaaacgt tatcaggtat gtttaaagct gttctgttgg gcaataccct
     4141 gcagtttcgg gtgatcactg agatatttca gggaggcttt gtagtcttcc agcaacagtt
     4201 cagcaaagtc catttcgaag ccgcgacgac acataatgcg catcaccacg atgtcggtgg
     4261 cttcaccgcc gagagtgaag gccggaacct gccagccgcg cagacgcaga cgttcagaga
     4321 ggtcatacag ggtgtatccc ggatcttcac catctttcag tttgaagcaa accgccggaa
     4381 tgccttcgtc cgggcgaccg gtacagatga actcatacgg ccccagtttg gcgatttcat
     4441 ccgccagata agcggcaacc tggtaagagg cgttctgtac tttggtatag ccttcacgac
     4501 cgaggcgcag gaattcatag tactgtgcaa ttacctgacc cgccgggcgg gagaagttga
     4561 tggcgaaagt accaatctga ccaccgaggt agtcaacgtt gaacaccagt tcctgcggca
     4621 gcgcttcttc atcacgccag ataacccagc cgcagcccag cggagccaga ccgaatttgt
     4681 ggcctgaagc actgatcgat ttcacacgcg gcaggcggaa gtcccagacg atatccgggg
     4741 cgacgaacgg tgccaggaag ccaccactgg cggcgtcgat gtgcatgtcg atgtcgatac
     4801 cggtgtcggc ctggaattta tccagcgcat cgtgcagcgg ttgcgggaac tcatagttac
     4861 cggtgtaggt cacgccgaaa gtcggcacca cgccgatggt attttcgtcg caggcttcaa
     4921 tcatgcgttt cggatccata aacaactgac cggggcgcat agggatctca cgcagctcca
     4981 catcccagta gcgggcgaat ttatgccagc agatttgcac cgggccgcac accaggtttg
     5041 gtttatccgt tggtttgcct gcagcttcca tacgcttgcg ccaacgccat ttcatcgcca
     5101 tcccgccgag catacaggcc tcggaagaac caatggtgtt ggtgccaacg gcctgaccat
     5161 ttttcggcgc aggcgcatgc cacagatcgg caaccatatt tacgcaacgc aggtcgatgg
     5221 ctgcagattg cggatattct tctttgtcga tccagttttt gttaatggat aaatccatca
     5281 acttgtggac gttttcgtcg tcccaggtct ggcagaaagt ggccaggttc tgacgagcgt
     5341 tgccatcaag atataattcg tcattgataa tctgaaatgc gacatcgtcg cgcatttcgt
     5401 gcagcggaaa acgttttgat tctg
//
LOCUS       DBFIFM010000250         5337 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.250, whole genome shotgun sequence.
ACCESSION   DBFIFM010000250 DBFIFM010000000
VERSION     DBFIFM010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.250"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..1392
                     /locus_tag="N7C74_004303"
     CDS             <1..1392
                     /locus_tag="N7C74_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGU2434049.1"
                     /translation="TDAVETAIPDNEKTECKVEVEPSVEREGPFYFLFTDKDGEKYGR
                     ANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNANTAQNSEQPEPVKVTADEVK
                     KIMQAANISQPDAEELLAVSRGEFVEGISDPNDPKWVKGIQTRDSVNQNQQETEQNDQ
                     KAEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGQSGGGNCPDCGAVMGDATYQEIF
                     DGENQPEVQENDPEEMEGTAHQHKENTGGNQHHASDSETGEASDPLIKANGHHNLTST
                     SRAGIHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLDTAGGVIDRDT
                     MKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVHVWGNGANFDNTILRR
                     SYERQGSPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVS
                     AIWQKLIPSQADS"
     gene            1465..1716
                     /locus_tag="N7C74_004304"
     CDS             1465..1716
                     /locus_tag="N7C74_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGU2434050.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            1751..1938
                     /locus_tag="N7C74_004305"
                     /pseudo
     CDS             1751..1938
                     /locus_tag="N7C74_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
     gene            complement(2413..2499)
                     /locus_tag="N7C74_004306"
                     /pseudo
     CDS             complement(2413..2499)
                     /locus_tag="N7C74_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2705..3031)
                     /gene="ynfA"
                     /locus_tag="N7C74_004307"
     CDS             complement(2705..3031)
                     /gene="ynfA"
                     /locus_tag="N7C74_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HGU2434051.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGPLIALCGMLIIV
                     VGWGRT"
     gene            3166..3507
                     /gene="ynfB"
                     /locus_tag="N7C74_004308"
     CDS             3166..3507
                     /gene="ynfB"
                     /locus_tag="N7C74_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HGU2434052.1"
                     /translation="MKITLSKRIDLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTGNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            3542..4102
                     /gene="speG"
                     /locus_tag="N7C74_004309"
     CDS             3542..4102
                     /gene="speG"
                     /locus_tag="N7C74_004309"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HGU2434053.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(4105..4815)
                     /gene="ynfC"
                     /locus_tag="N7C74_004310"
     CDS             complement(4105..4815)
                     /gene="ynfC"
                     /locus_tag="N7C74_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HGU2434054.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVGCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            4923..5228
                     /gene="ynfD"
                     /locus_tag="N7C74_004311"
     CDS             4923..5228
                     /gene="ynfD"
                     /locus_tag="N7C74_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HGU2434055.1"
                     /translation="MKLSTCYAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
BASE COUNT         1612 a         1255 c         1205 g         1265 t
ORIGIN      
        1 actgatgcag tggaaacggc aattccagac aacgaaaaaa ccgaatgcaa agtggaagtc
       61 gaaccatctg tagaacgtga ggggccgttc tacttcctct tcaccgacaa ggatggcgaa
      121 aaatacggtc gcgcaaacaa actttctggt ctggataagg cgctggctgc cggggctact
      181 gaaatcacaa aagaagaata ttttgcccga aaaaatggca catacacagg cttaccgcaa
      241 aatgcaaata ccgcacaaaa ttctgaacaa ccagaaccgg taaaagttac cgctgacgaa
      301 gtaaagaaaa ttatgcaggc agccaatatc agccagcctg acgccgagga actgcttgca
      361 gtatcacgtg gtgaatttgt tgaagggatt agcgacccga atgatccgaa atgggttaag
      421 gggatccaga cccgcgattc tgtgaaccag aaccagcaag aaacggaaca gaacgaccag
      481 aaagcggaac aaaacagccc aaatacgcaa caaaacgagc cagaaacgaa acaacctgaa
      541 ccagtagtgc aacaggaacc ggaaaagatc tgcaccgcct gcggtcagag cggtggcggc
      601 aactgccctg attgtggtgc ggtgatgggt gacgcaacat accaggaaat attcgatgga
      661 gagaatcagc ctgaagttca ggaaaatgat ccggaggaaa tggaaggtac tgcacatcag
      721 cacaaggaga acactggcgg caatcagcat catgccagcg atagtgaaac tggcgaggcg
      781 tcagatccct taattaaggc gaacggtcat cataatctca catccaccag cagagcgggg
      841 attcatctga tgatcgatct tgaaaccatg ggaaaaaatc ccgatgcccc gattatctca
      901 ataggtgcaa tatttttcga tccgcaaacc ggagatatgg gaccggaatt tagtaagact
      961 atcgatctgg atactgctgg cggagtcatt gatcgggaca ccatgaaatg gtggcttaaa
     1021 caatcacgcg aagcgcaatc tgccattatg accgatgaaa tcccgttaga tgatgcactg
     1081 ttacaattgc gggaatttat cgacgaaaac tccggtgaat tttttgttca tgtctgggga
     1141 aatggagcca acttcgacaa cacgattttg cgccgttcat acgaacggca ggggagcccc
     1201 tgcccgtggc gttactacaa cgatcgcgat gtacgcacaa tcgttgagct ggggaaagcc
     1261 atagacttcg atgccagaac ggctattcca ttcgaaggtg agcgccataa tgcacttgat
     1321 gacgcccgtt accaggcaaa atacgtttca gctatctggc aaaaactgat cccgagtcag
     1381 gctgattctt aatgttcaac tgtcgccggt tgtgactggt attctgcaac cggcgctcgt
     1441 ctgatgtaag agataaagaa atcgatgagc gaagtaatca tgattgtctc tcccggcaaa
     1501 tgggtatccg aagagcagtt aattgcgctg aaaggaataa aaaaaggtac gttaaaaaag
     1561 gcccgggaaa aatcgtttat ggaaggaagg gaatataagc atgtcgctca tgacggtatg
     1621 ccatgggata acagtccatg cttttacaac ctggaagaaa ttgatcgctg gattgagcgc
     1681 caggcatcag caagaccaag acgtcatctt acttgactaa aagccacact aactaatgag
     1741 agaagttgaa atgaaatatc cgacaggcgt ggaaaaccat ggagggaaat tacgtatctg
     1801 gtttgtttat aaaggcgtaa gagtcaggga aaacctgggg ttcctgacac agcaaaaaac
     1861 aggcgcgttg caggtgagct acgcgcctct gtttgttacg caataaaaac tggtgttttc
     1921 gactatgcaa aacagtttcc ctcctcacgc aatctggaaa aatttggtga ggcccgacaa
     1981 gatttaacca taaaagaact ggctgaaaaa tttctggcac tgaaagaaac tgaagtcgca
     2041 aaaacatcac tcaacacata ccgtgccgtc atcaaaaata tcctgagcat aatcggtgaa
     2101 aaaaatcttg cctcatcgat taataaagaa aaattactgg aggttcgtaa agagctactg
     2161 actggatacc agatccccaa aagtaactat attgttacac aaccagggag atcggctgta
     2221 actgtaaata attacatgac aaatcttaac gccgtgttcc agtttggtgt tgataacggt
     2281 tacctggcag ataacccgtt taaggggatc tcgccattaa aggaatcaag aaccattccg
     2341 gatcctcttt cgcgggagga attcatccgt cttatcgatg cgtgcagaaa tcagcaagcc
     2401 aaaaatttat ggtgtaatac cgtcctcagt ggtgattttt aatgtgacga aattacgccc
     2461 cggacaggta acaaaaactt cagcctttac gatcttcatg ttcgattcct tgcatcgctt
     2521 gtcgtgatgc atgaaatcta cgcaactgag ctactaccat ataagtatga agatcgaaaa
     2581 aagccggagt gatcacaaaa aaaggcgtat atttgcgctg tgaatggttg acaaaagatg
     2641 aaatagaata ccttttgtca gctgacactt cctcgtatct tattgataaa atggatttat
     2701 gttcctacgt gcgcccccag cccacaacaa tgatcaacat gccgcaaagc gcaatcaacg
     2761 gccccgtcca gtcataaaga gtcagtttca cgccatccac aacgcgcagc cacatcaacg
     2821 ccgtgcagac ataaacgcca ccataagccg cgtaaacacg cccactcgcc gcaggatgca
     2881 acgttaacaa ccagacaaac agcgccagtg aaatccccgc cggaagcaac agccagatac
     2941 tggcgtttcg ttttaaccac aaccagggca gaaagcatcc aataatttca cacagcgcag
     3001 tagcaaaaaa tagtaacgtt gttttaatca tctttgtcac ttattgacat catgtatagt
     3061 tatagggcga cataatatca tcaatataaa caccctcctg gtacgttttg cgttcgcagt
     3121 ggatggtgta gaatcatctt taatcattca tacagggaat gaattatgaa aatcactctc
     3181 agcaaacgaa tcgacctgct cgcttttctg ctgccttgcg cactggcatt gagcacaact
     3241 gttcatgccg aaactaacaa actggtgatt gagtctggcg acagtgcaca aagccgccag
     3301 cacgccgcta tggaaaaaga gcaatggaat gacacgggca atctgcgcca gaaagtgaat
     3361 aaacgcactg aaaaagagtg ggataaagcc gacgccgctt ttgataaccg cgataaatgc
     3421 gagcaaagcg ccaacatcaa tgcctactgg gagccaaata ctttgcgctg cctggaccgt
     3481 cgaactggcc gcgttattac cccctaacct gttattgatt taaggaatgt aaggacacgt
     3541 tatgccaagc gcccacagtg ttaagctacg cccgctggag cgtgaagatt tacgctatgt
     3601 acatcaactc gacaataacg ccagtgtgat gcgttactgg tttgaggaac cctacgaagc
     3661 ctttgttgaa ctctctgatc tgtatgataa gcatattcac gatcagagcg aacgacgctt
     3721 tgtggtggaa tgtgacggcg aaaaagccgg tctggtggag ttggtggaaa ttaaccatgt
     3781 acatcgccgc gcagaatttc agataattat ctccccggag tatcagggga aaggtctggc
     3841 aacccgtgcc gccaaattag caatggacta tggcttcacc gttctcaatc tctataagct
     3901 gtatctgatc gttgataaag agaatgaaaa agcgattcac atttaccgca agcttggctt
     3961 ttcggttgaa ggtgaattga tgcacgagtt ctttattaat ggtcaatatc gtaatgccat
     4021 tcgcatgtgt atattccagc atcagtatct ggcagagcac aaaacaccgg gtcagactct
     4081 cctgaagccg accgcacaat agcattaata ataatcgatc gtatttttga tggtgtaaac
     4141 ccgttcgacg gcgggtttta ctccttcatc aacaatgatc agctgacagc ccaccggatt
     4201 ggcgtgactg tcatattcac agctctgttt tacattacca actcgttgat tattcagtaa
     4261 agtaaccgct gtgtaatcta attttttgat cgggtccgtt gatggcgtgg cgctgacaga
     4321 taatgttttg tcgttacttt tcgtggtttt acccagtgga taaccctgat catcatagcg
     4381 atattccatc tgcatttgtt tgctgctggc tttaatcacg aagccattat catcggtttc
     4441 ccaactcacc ccggcagaag gtaattctgc tagctggcat tttccctgta aacgtactct
     4501 cttctccagc gtctcggcat cacggtaata attggcgtcc agtaccagag cgaccacggt
     4561 attattttcc agatccagta attcgagtga atcaaaacag ccttcttccg acaaagtccc
     4621 cgaaacacgt ttcgtcactt caccttgctc atccattaat gtctgagtga aatcttttac
     4681 cggaccacgc agcggatcaa aatcgaattc attagagaaa ctggccatct caggagtaaa
     4741 tgaaagtgtt acctctgtgc ggtcacatcc tgtgagtaat atcgcgagta agcatggcag
     4801 taatttgtat ttcacatcag tcaccagaga gtagagatga ttctcaatca tagtagcaaa
     4861 tacagtactt tacacgtgaa atgctatgct taaagaagtt atcttcgcgt taggagctta
     4921 tgatgaaact ctcaacctgt tatgccgcgc ttctgctcgc cctcgcctca cccgcggtac
     4981 tcgccgcgcc gggatcctgt gagcgcatac aaagcgatat atcacagcgc attatcaata
     5041 atggcgtacc cgaaagcagc ttcacgttaa gtattgtacc caatgaccaa gttgatcagc
     5101 ctgattccca ggtcgtcggg cattgcgcta atgatacgca taaaattctc tacacccgca
     5161 ccaccagcgg taacgtctct gctcccgcgc agtctaccca ggatggcgcg cctgccgaac
     5221 cgcagtaata cattatcgac tacacgccgg atacgacaca tccggcgatt tggacataca
     5281 acataatccc accatattac tcataccctt ctattgatat ggattaataa ttattaa
//
LOCUS       DBFIFM010000251         5309 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.251, whole genome shotgun sequence.
ACCESSION   DBFIFM010000251 DBFIFM010000000
VERSION     DBFIFM010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.251"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            92..186
                     /locus_tag="N7C74_004312"
     tRNA            92..186
                     /locus_tag="N7C74_004312"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:126..128,aa:Sec,seq:tca)
     gene            382..1563
                     /locus_tag="N7C74_004313"
     CDS             382..1563
                     /locus_tag="N7C74_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2434056.1"
                     /translation="MALTDVKVKTAKPKERPYKLADGGGMYLLINANGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKILAAGGDPGEAKKEEKIALQMSLKNTFEA
                     VAREWHQTKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLTD
                     LAGYTGSIITKTATQIIMLTGVRTQELRFAHWEDIDFEAKLWEIPAEVMKMKRPHIVP
                     PSEQVIALFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHAQYLDGRREMMQWYADYIDSLSEL
                     A"
     gene            1662..2878
                     /locus_tag="N7C74_004314"
     CDS             join(1662..1967,1970..2878)
                     /locus_tag="N7C74_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611429.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc31 family transposase"
                     /protein_id="HGU2434057.1"
                     /translation="MISSPQHKTGDLMNKKTKRTFTPEFRLECAQLIVDKGYSYRQAS
                     EAMNVGSTTLESWVRQLRRERQGIAPSATPITPDQQRIRELEKQVRRLEEHNTILKKA
                     NRALDVRLAERFTIVARLSDSHSVVSLCSALEIHRSSYQYWRKRRDTVNPARVRLCSE
                     IRRAWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQE
                     HTCLPNLLERQFAVPEPDRVWCGDITYIWAGNRWCYLAVVMDLFARRVIGWSLSANAD
                     TALISSALRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLLWRYRIKQSVSRRGNCWDN
                     SPMERFFRSLKTEWVPTDGYTGKDVARQQISSYILNYYNSVRPHHYNGGLTPEESENR
                     YHFYCKTVASIT"
     gene            2887..3039
                     /locus_tag="N7C74_004315"
                     /pseudo
     CDS             2887..3039
                     /locus_tag="N7C74_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
     gene            3129..3503
                     /locus_tag="N7C74_004316"
     CDS             3129..3503
                     /locus_tag="N7C74_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGU2434058.1"
                     /translation="MKTLPDTHVREASRCPSPITIWQTLLGRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEAK"
     gene            3500..3991
                     /locus_tag="N7C74_004317"
     CDS             3500..3991
                     /locus_tag="N7C74_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312567.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGU2434059.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDIDGVNLAELINMVCDNGY
                     SLRVVDESDRTSADCTPPFTALTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGNGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAGCLPGLPT
                     FNW"
     gene            4003..4200
                     /locus_tag="N7C74_004318"
     CDS             4003..4200
                     /locus_tag="N7C74_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGU2434060.1"
                     /translation="MTSLTPEAALDILIAWLQDNIDSESGIIFDNDEDKTDSAALLPC
                     IEQVREDVRTLRQLQLLQQNR"
     gene            4285..4626
                     /locus_tag="N7C74_004319"
     CDS             4285..4626
                     /locus_tag="N7C74_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434061.1"
                     /translation="MSGIIISRPEVDTGHTDVICSTSIRHVVTVRNAALQQTETLIRQ
                     LAEISVLTAADIGGKTALDRAMKQDFHCGCWLMEKPETAMKAITRNLDCEIWRDLMQR
                     SGMLSLMDAQG"
     gene            4687..4830
                     /locus_tag="N7C74_004320"
     CDS             4687..4830
                     /locus_tag="N7C74_004320"
                     /inference="COORDINATES: protein motif:HMM:NF025085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434062.1"
                     /translation="MNAFKQLHQSKDEVFERGVINVFRGPNGITKPIPPVNSAVKLSS
                     TTW"
     gene            4824..5243
                     /locus_tag="N7C74_004321"
                     /pseudo
     CDS             4824..5243
                     /locus_tag="N7C74_004321"
                     /inference="COORDINATES: protein motif:HMM:NF025085.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
BASE COUNT         1443 a         1320 c         1327 g         1219 t
ORIGIN      
        1 gtgtattagg cggaaaaaac tgatctgggg gatgtagaaa ctcaaggaag tagctataat
       61 gcgccccgcc tccatgtagc aatcgaggcg cggaagatcg tcgtctccgg tgaggcggct
      121 ggacttcaaa tccagttggg gccgccagcg gtcccgggca ggttcgactc ctgtgatctt
      181 ccgccagttt gcctctcctg aactctcccg acatcaacaa agccccttat aatcccttat
      241 atatcaggtt ttctttgtat cctgaggtca aacgacttta acccatatca agttgtttgt
      301 gggggtgcat tgggggtatc aggttggtta ctggcggaaa ttcccccagg aggatggatt
      361 catcaaaagc tggaggatgt catggcgcta accgatgtta aagtgaaaac cgcgaagcca
      421 aaagaaaggc cctataagct ggctgatggc ggaggtatgt atctgctgat taacgctaat
      481 ggttccaaat actggcgtat gaagtaccgc ttcgccggta aagaaaagat gctgtctatt
      541 ggcgtatacc ccgatgtaac gcttgccgac gcacgtgaga aacgcagtga agccaggaaa
      601 atacttgcgg ctggtggcga tccgggtgag gcaaaaaaag aagaaaaaat agcgctgcaa
      661 atgagtttga aaaacacttt cgaggccgtt gctcgcgagt ggcatcagac gaaagccgat
      721 cgctggtcac tgcgctaccg cgatgaaata atcgacacct ttgagaaaga tatcttcccc
      781 tacatcggca agcgacctat tgccgaaatc aagccgatgg agctattgga agcactacgt
      841 aagatggaga aacgtggagc actggagaaa atgcgcaagg tccgccaacg ttgtggtgag
      901 gtgtttcgct atgcaattgt tactggtcga gccgactata accctgctcc cgatcttgcc
      961 agtgctttag ctacaccgaa aaaagtacat tttccctttc ttactgccaa tgaacttccc
     1021 cacttcctta ctgatctggc gggttatacc ggaagtatca tcactaagac agctactcag
     1081 atcattatgt taaccggtgt acgaacgcaa gagttgcgtt tcgcgcattg ggaggatatt
     1141 gattttgagg caaaattatg ggagatcccg gcagaagtaa tgaagatgaa acggcctcat
     1201 attgtgccgc cctctgagca ggttattgcg ctatttaagc aacttgaacc aatctcaaaa
     1261 catcatcctc tggtctttat cggtaggaac gatcctcgca agccaattag taaggagagc
     1321 attaaccagg tcattgaatt gctggggtat aagggaagac tcacagggca cggtttcagg
     1381 cataccatga gcacaattct gcacgaacaa ggctttaatt ctgcctggat tgaaatgcag
     1441 ttggctcatg tggataaaaa ctccatcagg ggtacctata accacgccca gtatctcgat
     1501 ggtcgccgtg aaatgatgca atggtacgct gactacattg attcactttc tgagctggcc
     1561 tgatgaatga aagttcggtg acacaaaaat aatgaataac catccagccc caacaggcgg
     1621 atggttgtag tggcacagta aatttggcca cctgattaaa ggtgatatcc tcaccacaac
     1681 acaaaacagg tgacttaatg aacaagaaaa ccaaacgtac tttcacccct gaattcaggc
     1741 tggaatgtgc acagctaatt gttgataagg gctactcata tcgacaagcc agtgaagcga
     1801 tgaatgtcgg ttctaccacg cttgagagtt gggtgcgcca gctcaggcga gagcgtcagg
     1861 ggattgcgcc ctctgccaca cctattactc cagaccagca acgtatccgc gaactggaaa
     1921 agcaggttcg ccgcctggag gaacacaata cgatattaaa aaaggctaca accgcgctct
     1981 tgatgtccga ctcgctgaac ggttcacgat agttgccaga ctgagtgaca gccactcagt
     2041 tgtcagcctc tgttctgctc tggaaataca ccgcagcagt taccagtact ggcgaaaacg
     2101 acgcgatacg gttaatccgg cgcgagtcag gttgtgcagc gaaatacgcc gggcgtggaa
     2161 ccaaagtagg ggctctgcgg gggcgcgcac gctggctgaa atgctgaccc aaaacggcgt
     2221 cccgatgagc cgttaccgtg ccgggcgtct gatgaaatat ctgaacctga gcagttgtca
     2281 gcccggaaaa catcagtaca aaaatgctcg tcaggaacat acctgcctgc cgaatctgct
     2341 tgagcgccag ttcgctgtgc ccgagccaga tagggtatgg tgcggagata ttacgtatat
     2401 ctgggcagga aatcgctggt gctatctggc ggtcgtcatg gatctttttg cccgcagggt
     2461 tatcggctgg agcctgtcag cgaatgccga tactgccctg ataagcagtg ccctgcggat
     2521 ggcgtatgag gtgcgtggtc agccgcggga cgtcatgttc catagcgacc agggaagtca
     2581 atatacagga ctgaaatatc aacaacttct ctggcgttac aggataaagc aaagtgtcag
     2641 ccgacgggga aactgctggg ataacagtcc aatggaacgc ttcttccgca gtctgaaaac
     2701 agaatgggtg ccaaccgatg gctacacagg caaagacgtg gcccggcagc aaatcagcag
     2761 ttatattctg aattattaca atagcgtcag gcctcaccat tataacggag ggctgacgcc
     2821 ggaagagtca gagaacagat atcattttta ctgtaaaacc gtggccagta ttacttgacc
     2881 actacactgg aactcatgct caccagcggt gagctgaatc cccgccatca gcataccgtc
     2941 acgctgtatg caaaagggct gacctgcaaa gccgataccc tcggcagtcg aggttacgtt
     3001 tatatggctg tttatccgac gcccgaaacg aaaaagtaaa tcatcagaat aaccttctgc
     3061 tccggcctgg tgttttcacc acgccacttc cccatttctc atctctgcat atcaggaaaa
     3121 actttagtat gaaaacatta cccgatacac acgtacggga ggcatcgcgc tgcccgtctc
     3181 ccatcaccat ctggcagaca ctgcttggcc gtctgctgga ccagcactac ggcctcacgc
     3241 tgaatgacac accgttcgct gatgaacgtg tgattgagca gcatattgag gcaggtattt
     3301 cactgtgtga tgcggtgaac tttctcgtgg aaaaatacgt gctggtgcgt accgaccagc
     3361 cgggattcag cgcctgtacc cgctctcagt taataaacag catcgatatc ctccgggctc
     3421 gcagggcgac cggcctgatg acccgcgaca attacagaac ggtaaataac attaccctgg
     3481 gtaagcatcc ggaggcgaaa tgatgaaact ggccctgaca ctggaagccg acagcgttaa
     3541 cgtacaggca ctgaatatgg ggcgaattgt cgttgacatt gatggtgtta atctcgctga
     3601 actgattaac atggtctgcg ataacggcta ctcccttcgt gttgttgatg aatctgaccg
     3661 gacctcagca gactgcacgc caccatttac tgcccttacc ggcatacgct gcagtaccgc
     3721 acatatcacg gaaacggaca acgcctggct gtactcgctg tcacaccaga ccagtgactt
     3781 cggtgaatca gaatggattc attttaccgg taacggctat ctgttgcgta ccgatgcgtg
     3841 gtcgtacccg gttctgcggc ttaaacgcct gggtctgtca aaaacgttcc gtcgtctggt
     3901 cgtcacactc atccggcgtt atggtgtcag tctcattcat ctggatgcca gtgccggatg
     3961 tctgccaggt ttacccactt ttaattggta actaggaaca acatgacatc attaaccccg
     4021 gaagcagcac tggatattct gattgcgtgg ctgcaggaca atatcgacag cgaatccgga
     4081 attatcttcg acaacgatga ggataaaacg gattcggcag cattgttgcc ctgtatcgaa
     4141 caggtcaggg aagatgtccg taccctgcgc caactgcagc ttctgcaaca gaaccggtga
     4201 gtctcactca ccatacttca ttccactgac gccagcctga acagggctgg cgttttcgtt
     4261 tataaaccaa aaggaaaacc gataatgtca ggcatcataa tctcccgtcc ggaagtggac
     4321 accgggcata cggacgttat ctgctccacc agtatccgac acgttgtgac ggtacggaat
     4381 gcagcactgc aacaaaccga aacgcttatc cggcaactgg cagaaatctc agtcctgaca
     4441 gcagcagaca ttggcggcaa aaccgccctg gaccgggcga tgaaacagga ttttcactgc
     4501 ggctgctggc tgatggagaa acctgaaacc gcaatgaaag ccatcacccg caacctcgac
     4561 tgcgaaatct ggcgtgacct gatgcaacgc tccgggatgc tgagtctgat ggatgcacag
     4621 ggctaggata catggtaccg ctcactggag tacgataata ttccggaaat cagcgaggcg
     4681 aatatcatga atgcattcaa acaactgcac cagagtaagg atgaagtgtt tgagcgtggg
     4741 gtgatcaacg tcttcagagg gccgaacgga attacaaaac caattccccc tgtaaattcg
     4801 gcagtaaaat tatcgtcaac aacttggtga gatgggacag atggagattt catcttatca
     4861 gtggaatgca ggcagatcgc ttagctgacc tggaaagaat gttgcatctg ctcagcggta
     4921 aaccgctccc caacaaccga gggaatatca ccattaatct ggatgaccac ctacagtccg
     4981 ttcagggtaa agagcgctat gaagacaagt aaatacttta agaagggccc gcacacatca
     5041 cattcagaaa gccagagctg gttgacaggc ttaatgatat tattgccaga cactacctag
     5101 gaatgttacc ttcacaaaaa tctctaagaa ttgattcaat tgtgtatttt agcctcattc
     5161 cctcatgtaa agtcatttgg gggttattat gggggtcaat catttatcca acacgcatta
     5221 atcatttaaa atcaatgcat taataatcat attcgactcc tgtgatctcc gccaaacttc
     5281 ctcttctgaa ctctcccgac gtcaacaaa
//
LOCUS       DBFIFM010000252         5304 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.252, whole genome shotgun sequence.
ACCESSION   DBFIFM010000252 DBFIFM010000000
VERSION     DBFIFM010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.252"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            221..1042
                     /locus_tag="N7C74_004322"
     CDS             221..1042
                     /locus_tag="N7C74_004322"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGU2434063.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWLKRRLFYYTTPLFSSVLALHKGPRLPDN"
     gene            1042..2148
                     /locus_tag="N7C74_004323"
     CDS             1042..2148
                     /locus_tag="N7C74_004323"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGU2434064.1"
                     /translation="MMKILFTESSSDIGGQELQALAQMTALQKQGHSVLLACREKSKI
                     APEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLIC
                     CHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDW
                     PALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFR
                     WLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGM
                     VLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMM
                     AASAREDIEYRFDINRTAQIIVSLASQAKGKCNR"
     gene            2238..3959
                     /gene="cptA"
                     /locus_tag="N7C74_004324"
     CDS             2238..3959
                     /gene="cptA"
                     /locus_tag="N7C74_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HGU2434065.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETTRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            4033..5031
                     /gene="lpxM"
                     /locus_tag="N7C74_004325"
                     /gene_synonym="msbB"
     CDS             4033..5031
                     /gene="lpxM"
                     /locus_tag="N7C74_004325"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HGU2434066.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSARQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLMAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
     gene            5084..5272
                     /locus_tag="N7C74_004326"
                     /pseudo
     CDS             5084..5272
                     /locus_tag="N7C74_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1357 a         1176 c         1334 g         1437 t
ORIGIN      
        1 ttctgaaaaa cagaaaatct gattaaaaag agcatatgcg ttaagggaaa tttaagttct
       61 ggttgattaa ctgataaaaa tattaaaaaa tccctgttgt cggtaatctc cggaccattc
      121 cgggattaca caataatatc accatgtgtg atatctatgg atcctcactg ttgtttacgc
      181 gaggatattt tcagaatgct taacagacga ttattttttt atgttgaatg caaggcatct
      241 tcccgtactg atgtatcatc atgtcagtcg ttgtccgggg cttgtgacac tctcacctga
      301 aacattttgt gaacagatga agtggctcgc agataataac tggcggacgg taacatctgc
      361 tgagcttgaa tatttttatc aggggggaac gcttccacga aagagtgtca tgctcacatt
      421 cgatgatggt tatcttgata actggctcag agcatggccg gtcctgaagg aatataacct
      481 tcatgcacat atttttctca ttacggggct gattggtaag ggaaacgtac gtagcaggca
      541 ggagcaggaa tattctcacc gcgactgtga acggttgatt gccgataacc gttcagatga
      601 ggtcatgctt cgctggtctg aagtccggga aatgcgtgat tccggactgg ttgaatttca
      661 tgtgcacacc cattcacata aacgctggga caggttgtct gtatcacgtg ctgaacagtg
      721 ccggctcatg aaagaagata ttctggtggg aaaacaatgt ctgacggaaa agctggggtt
      781 ctgtagttcc catctgtgct ggcctgaagg atattataat cgggattata ttaatctcgc
      841 cggtaagctt ggtttctctt atttatatac aaccgaaaga agaatgaact gtcctgaaaa
      901 tggttcatta cgcatagggc gtatcagcac caaagagcgg gaacattccg gctggctgaa
      961 acgacgcctt ttttattata caacgccact tttttcttca gtactggctc ttcataaggg
     1021 gccaagatta ccagataatt aatgatgaaa attttattca ctgaatcttc gtcagatatt
     1081 gggggccagg agctgcaggc actggcccag atgactgctc ttcaaaagca gggacattcc
     1141 gttttacttg cctgcagaga aaaaagtaaa attgctccgg aagccagaaa aagagggcat
     1201 gatgtcactt ttattccttt caggaacagc cttcaccttc cttccatcct cagacttcga
     1261 cggattatcg gggaatttaa acctgatctg gtcatctgcc atagtggcca tgacagtaat
     1321 atagcaggac tgagccgtct gatatgctgt caccgcttca gtattgtcag acaaaaaacc
     1381 tacatcaccc gtaagaccag aacgttttcc ctgaattatc tgtgtgattt cattgttgtg
     1441 cctagctcag ccatgatggc gcatctgatg gcggaaggtg tcaggacacc ggtaacggtt
     1501 attccaccgg gtttcgactg gcccgcattg cataatgaag ccatgcgacc gttacccctg
     1561 catatccacg cctgggctgc atcagcagac aatgttccgc ttatcgttca ggtggggatg
     1621 ctacgtcccg aaaaaggaca tgaatttatg ctgcgtgtat tgtatcagct gaagatggaa
     1681 gggaaatcat ttcgctggct cgtggtgggc gccgggcgtg aagaatacga agcacgtctg
     1741 cgtcagcaga cagaacacct gggaatgagc ggagatgtgt taatggcagg tgcccttttc
     1801 ccagcgttac cggtctaccg gattgccagt gtggtggtca tgccttcgga aaatgaagcg
     1861 tttggtatgg tactggctga agcgtcagtc tccggcgtgc ccgttatcgc cagtgagacg
     1921 ggcgggatcc ctgatgtcat tcagaagaat gtgacaggga cgctgttacc cgtgggggat
     1981 gtgtctgcat ggaccggcgc actgcgcgat tttttatccc gaccggaacg cttccggatg
     2041 atggcagcca gcgccagaga agatattgag taccggtttg atattaacag aacggcacag
     2101 atcattgtgt ctctggctag tcaggctaag gggaaatgca acagataacg gcggatcctc
     2161 ttttcagccc gaattttgtt cagcggtttt ccggtaacgg agtaatttat cacattgttt
     2221 aaatacgggg acggtcaatg catttaaata ccggacagaa caggcctact ttcagctgga
     2281 gcgcgctggg atgggcaatt ttttattttg gctttttttc cactctcctg caggtcatca
     2341 tcttcagcag tgggtacagc ggaacgaatg gaatacggga ctcactgtta ttcagttgtc
     2401 tgtggttgat cccggtgttt ctctatcctg atcggataaa aataattgca gctgttgtcg
     2461 gtttcattct ctggggcacg tcgctggcag cactttgtta ttattttctc tatggtcatg
     2521 aattctctca aagtgttctt ttcgttatgt ttgaaacgaa tgccagagag gctggtgaat
     2581 atttcagcca gtatttcagc cttaaactgt tgcttatctc gctggtatat actgcggtgt
     2641 ccgtttttct gtggacacgt ctgcgtcctg tatatattcc tttgccatgg cggagaattg
     2701 tctctttcct gctgctttat gctctgcttc tgcatccggt tgttctgaaa tcgttaatca
     2761 gacaggagcc gctgaatgat actctaggca aactggcatc ccgaatggag cctgctgccc
     2821 cctggcagtt tgtatccagt tattatcagt accatcagca actgaatgca ctgacaacct
     2881 tcctgaatga aaatagcgca ctgccaccac tgggtaatct cagggatgaa tcaggggaga
     2941 gaccacgcac actggtcctg gtgattggtg agtcgacaca gcgcgaacgc atgagcttat
     3001 acgggtatct acgtgaaacg acgccggagc tggatgcact gcgtaaaacc gatccgggtc
     3061 ttactgtgtt taataatgtg gtggcatcgc gtccgtacac cattgaagca ttgcaacagg
     3121 cccttacttt cgccaacgaa aagaatcctg atctgtatct gacgcagccg tcgctgatga
     3181 acatgatgaa gcaggcaggc tataaaacat tctggattac caaccagcag acaatcacag
     3241 cccgtaacac catgctcact gtattttcgc gccagacgga caggcagtac tacatgaatc
     3301 agcagcgaac acaaagtgcg cgtgaatatg acactaacgt gctgaagccg ttccgggaag
     3361 tgctgaatga ccctgcacca aagaaactga tcatcgtgca tctgctgggt acgcacatta
     3421 agtataaata ccgttatccg gaaggtcagg ggcggtttga tggcattaca gggcatattc
     3481 ccactggatt aaatgcgaaa gagctggaag tgtataacga ttacgataat gccaatctgt
     3541 ttaacgatca tgtggtggcc agtctgataa aggacttcag agcaactgcg ccggacggct
     3601 ttctgcttta tttttcagac catggtgaag aagtatatga tactccgcca tataaaacgc
     3661 agggacggaa tgaagataac cccacacgtc ccatgtacac tgttccgttc ctgctgtgga
     3721 cctcggaaaa gtggcatgct gcacatccgc gagatttttc gcagtatgtt gaccgcaaat
     3781 acagtctggc tgaactgatc cacacctggt cagatttggc gggactgaca tacgatggtt
     3841 acgatccgac ccgttctctg gtgaatccgc aattcaggga aaccacccgc tggattggaa
     3901 atccgtataa aaagaatggg ctcactgatt tcgacactct tccgtatggt gagccatagt
     3961 attttcttat cttcggttcc gttctgaagg caggaggatg tgttttcctc tgctgtccgg
     4021 tggcctccag ggatggtacc accttctgct gtactctgtt atcataatga gataagcagg
     4081 cagataccgg taaatatgaa aaatatcaga actgagttta ttccccgctt taaccttaca
     4141 ttatgcttcc cccgttactg gatgacgtgg acaggtatcg gtattatctg tgtgtttgca
     4201 atggtccccc ccgcactgag agatccattg ttgggaaaat tgggcatgct ggttgggcgt
     4261 ctggggaaaa gcgcacgtca acgggccttg attaatttat cgctttgttt tccggagtac
     4321 agtgataaag aaaaggagaa tatcgttgat gccatgtttg caacagcctc catggccgtt
     4381 gtgcttatgg ctgaattagc actgtccggg cctgataaaa tttcgcatcg tatacgctgg
     4441 aatggtctgg agattgtgga gaagatggca cagaacaatg aaaaggtcat ttttcttgtg
     4501 ccacatgcct ggggagtgga tattcccgca atgctgatgg ctgcgtcggg cagaaaaatg
     4561 gcagcgatgt ttcataacca gagaaatccg gtggtggatt atgtctggaa cagtgttcgt
     4621 cgtcgtttcg gaggaaaact gcacgcacgt aatgacggta ttgcctcctt tgtccgctca
     4681 gtccggcagg gatactgggg atattatctt cctgatcagg atcatggccc ggaattcagt
     4741 gagtttgcag atttttttgc gacgtataaa gcaactttac cggttatcgg acggctcagc
     4801 aggatcagtg gtgcacggat aataccgctt ttccctgttt atgacggaaa gacccatcat
     4861 ctgactattc atgtcagccc gccacttgct atacgacaga agagtgatgc tcatattgcg
     4921 cgacagataa acgaagtggt tgaaaatttc gttcgtccac atcctgagca gtatacgtgg
     4981 atcctcaagt tgctgaaaac gcgtaaagaa ggggaagagg atccttactg aggcagcttt
     5041 ttcaggataa tgagcttacc cagcaatagt ggacacacgc taagtgagta aactctcagt
     5101 cagaggtggc tcatatgaca aaaaaacagt accaaccagt aaaaaacccc gtaaatagca
     5161 ttcgcctgaa tttcgcagtg aagccctgaa gcttgctgaa cgcatcggtg ttgctgtcgc
     5221 agctcgtgtg ctcagtctgt atgaatcaca gcttgtaagc ggctcgtcag aactgtattg
     5281 atatttactg agcggtcagt tcaa
//
LOCUS       DBFIFM010000253         5297 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.253, whole genome shotgun sequence.
ACCESSION   DBFIFM010000253 DBFIFM010000000
VERSION     DBFIFM010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.253"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..546
                     /locus_tag="N7C74_004327"
     CDS             <1..546
                     /locus_tag="N7C74_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU2434067.1"
                     /translation="KTGAEAIRALAMQIPAFRQKLSDGWYLVRIAGRDTGENELSARL
                     NEPLANGAVIHIVPRLAGAKSGGVFQAVLGAALIAVAWWNPVGWLGAAAVSGMYAAGA
                     SMILGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRV
                     VSQEISTADEGDGGQVVVIGR"
     gene            607..4020
                     /locus_tag="N7C74_004328"
     CDS             607..4020
                     /locus_tag="N7C74_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HGU2434068.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALRETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYDPEKRTYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWTDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEGIVDNGAHFDGDQSSTVNGVTPPAVQHL
                     TAEVSADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSQIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRIADIRQVETSARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPVFSLTSDGKLTAKNADISGSVNANSGT
                     LNNVTVNENCTIKGMLEATQVRGDFVKAVSKSFPKQAGTWGNTETPNGTVTVTISDDH
                     NFDRQIIIPPIIFNGIAYSDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGS
                     YSAVIDMPSGRGSVTLEFKVFHKGNQRAGNITDCTVIVTKKAASGISIR"
     gene            4091..4690
                     /locus_tag="N7C74_004329"
     CDS             4091..4690
                     /locus_tag="N7C74_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU2434069.1"
                     /translation="MRKVCAAILSAAICLAVSGVPAWASEHQSTLSAGYLHASTDAPG
                     SDDLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDYVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYFRVTDNKRKTHDVLTGSDDARYSNTSLAW
                     GAGVQFNPTESVAVDVAYEGSGSGDWRTDGFIVGVGYKF"
     gene            4750..>5297
                     /locus_tag="N7C74_004330"
     CDS             4750..>5297
                     /locus_tag="N7C74_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HGU2434070.1"
                     /translation="MAAVQISGVLKDGAGKPIQNCTIQLKARRNSTTVVVNTVASENP
                     DEAGRYSMDVEYGQYSVTLLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMSEDDVRPE
                     ALRRFELMVEEAARHAEEAKKNAGEAETSARNAGISASQAEESAANADTSAGDASESA
                     RQAAESAAAAKQSEEASSSSASA"
BASE COUNT         1243 a         1288 c         1684 g         1082 t
ORIGIN      
        1 aaaacggggg ccgaagccat ccgggcgctg gccatgcaga tcccggcgtt tcgtcagaag
       61 ctgagcgacg gctggtatct ggtacggatt gccgggcgtg atacaggtga aaatgaatta
      121 tctgcccgtc ttaatgagcc gctggcaaat ggtgccgtga tccacatcgt gccgcgcctg
      181 gcgggtgcta aaagtggcgg tgtgtttcag gcagtgctgg gtgcggcgct gattgcggtg
      241 gcatggtgga accctgtggg ctggctgggg gccgcggctg tatcgggtat gtatgcagca
      301 ggggccagta tgatcctggg tggtgtggcg cagatgctgg caccgaaagc cagaactccc
      361 cgtacacaga caacggataa cggcaaacaa aacacctatt tctcctcact ggataacatg
      421 gttgcccagg gcaatgttct gcccgttctg tacggtgaaa tgcgcgtggg atcacgtgtg
      481 gtttctcagg agatcagcac ggcagacgaa ggggacggtg gtcaggttgt ggtgattggt
      541 cgctgatgca aaatgtttta tgtgaaaccg cctccgggcg gttttgtcgt ttatggagca
      601 tgacgaatgg gtaaaggaag cagtaagggg cataccccgc gcgaagcgaa ggacaacctg
      661 aagtccacgc agctgctgag tgtgatcgat gccatcagcg aaggaccgat tgaaggtccg
      721 gtggatggat taaaaagcgt gctgctgaac agtacgccgg tgctggacag tgaaggtaat
      781 accaacatct ccggtgtcac ggtggtgttc cgggccggtg agcaggagca gacaccgccg
      841 gagggatttg aatcctccgg ttctgagacg gtgctgggta cggaagtgaa atacgacacg
      901 ccgatcaccc gcaccatcac gtcggcaaac atcgatcgtc tgcgctttac tttcggtgtg
      961 caggcactgc gggaaaccac ctcaaagggg gaccggaatc cgtcggaagt ccgcctgctg
     1021 gttcagatac agcgtaatgg tggctgggtg acggaaaaag acatcaccat taagggcaaa
     1081 accacgtcgc agtatctggc ctcggtggtg gtggataacc tgccgccgcg cccgtttaat
     1141 atccggatgc gcaggatgac gccggacagc accacagacc agctgcagaa caaaacgctc
     1201 tggtcgtcat acaccgaaat catcgatgtg aaacagtgct acccgaacac ggcactggtt
     1261 ggcgtgcagg tggactcgga gcagtttggc agtcagcagg tgagccgtaa ttatcatctt
     1321 cgcgggcgca ttctgcaggt gccgtcaaac tatgatccgg aaaaacgcac ttacagcggc
     1381 atctgggacg gaacgttaaa accggcatac agcaacaaca tggcctggtg tctgtgggat
     1441 atgctgaccc acccgcgcta cggcatgggg aaacgtcttg gtgcggcgga tgtggataaa
     1501 tgggcgctgt atgtcatcgg ccagtactgc gaccagtcag taccggacgg ctttggcggc
     1561 acggagccgc gcatcacctg taatgcgtac ctgaccacgc agcgcaaggc gtgggatgtg
     1621 ctcagtgatt tctgctcggc gatgcgctgt atgccggtat ggaacgggca gacgctgacg
     1681 ttcgtgcagg accggccgtc ggataaggtg tggacctata accgcagtaa tgtggtgatg
     1741 ccggatgatg gcgcgccgtt ccgctacagc tttagcgccc tgaaagaccg ccataatgcc
     1801 gttgaggtga actggactga cccggacaac ggctgggaga cggcgacaga gcttgtggag
     1861 gacacgcagg ccattgcccg ttacggtcgt aacgtcacga agatggatgc ctttggctgt
     1921 accagtcggg ggcaggcaca ccgtgccggg ctgtggctga ttaaaacgga actgctggaa
     1981 acgcagaccg tggacttcag cgtgggcgca gaagggcttc gccatgtacc gggcgatgtc
     2041 attgaaatct gtgatgatga ctatgcgggg atcagcatcg gtgggcgtgt gctggcggtg
     2101 aacagccaga cccggacgct gacgctcgac cgtgaaatca cgctgccatc ctccggcacc
     2161 acgctgataa gcctggttga cggaagtggc aatccggtca gcgtggaggt tcagtccgtc
     2221 accgacggcg tgaaggtaaa agtgagccgg gttcctgacg gtgttgctga atacagcgtg
     2281 tgggggctga agctgccgac gctgcgccag cgcctgttcc gctgcgtgag tatccgtgag
     2341 aacgacgacg gcacgtatgc catcactgcc gtgcagcatg tgccggagaa agagggcatc
     2401 gtggataacg gggcgcactt tgacggtgac cagagcagca cggtgaatgg tgtcacgccg
     2461 ccagcggtgc agcacctgac cgccgaagtc tccgcagaca gcggggaata tcaggtgctg
     2521 gcgcgatggg acacgccgaa ggtggtgaag ggtgtgagct tcctgcttcg cctgaccgtg
     2581 gcagcggatg acggcagtga gcggctggtc agcacggccc ggacgacgga aaccacatac
     2641 cgcttcacgc agctggcgct ggggaactac aggctgacag tccgggcggt aaatgcgtgg
     2701 ggacagcagg gcgatccggc gtcggtatcg ttccggattg ccgcaccggc agcgccgtca
     2761 cagattgagc tgacaccggg ctattttcag ataaccgcca cgccgcatct tgcggtttat
     2821 gatccgacgg tacagtttga gttctggttc tcggaaacgc ggattgcgga tatcaggcag
     2881 gttgaaacca gcgcgcgtta tcttggtacg gcgctgtact ggatagccgc cagtatcaat
     2941 atcaaaccgg gccatgatta ttacttttat atccgcagtg tgaacaccgt tggcaaatcg
     3001 gcattcgtgg aggccgtcgg tcgggcgagc gatgatgcgg aaggttatct ggattttttc
     3061 aaaggcaaga taaccgaatc tcatctcggc aaggagctgc tggaaaaagt cgatctgacg
     3121 gaggataacg ccagcagact ggatgagttt tcgaaagagt ggaaggacgc taacgataaa
     3181 tggaatgcca tgtggggcgt caaaattgag cagaccaaag acggcaaaca ttatgtcgcg
     3241 ggtattggcc tcagcatgga ggacacggag gaaggcaagc tgagccagtt tctggttgcc
     3301 gccaatcgta tcgcgtttat tgacccggca aacgggaatg aaacgccgat gtttgtggcg
     3361 cagggcaacc agatattcat gaacgacgtg ttcctgaagc gcctgacggc ccccaccatt
     3421 accagcggtg gaaatccacc ggtattttcc ctgacatcag acggaaagct gaccgctaaa
     3481 aatgcggata tcagtggcag tgtgaatgcg aactccggga cgctcaacaa cgtcacggta
     3541 aatgaaaact gtacgattaa gggcatgctg gaggcgactc aggtcagagg tgacttcgtt
     3601 aaagctgtat ccaaatcatt tccgaaacag gctggtacgt ggggtaacac ggaaacacca
     3661 aacgggacgg ttacagtcac catcagcgat gatcataact ttgaccgtca aatcattatt
     3721 ccgcccatta tctttaacgg aatagcgtat agcgatccgg gaagtggtaa taacccggga
     3781 ggtacaagat acacgggtta tggttttgaa gttcgcaaaa acggtgtatt aatcgcatcc
     3841 agagaaacta aaggggccat tcccggtagc tacagtgcgg ttattgatat gccgagtggc
     3901 aggggaagcg tcactctgga gtttaaggtt ttccataaag gcaatcagcg ggcaggtaat
     3961 atcaccgact gtacggtgat tgtgaccaaa aaagcggctt ccggcatcag tatccgttga
     4021 aatatttata accctaataa cgggcgccag aaatggcgcc ttttttattg cagaaaatcg
     4081 ggaggtaatt atgcgtaaag tttgtgcagc aattttgtcc gcagccattt gtctggccgt
     4141 atccggtgtg cctgcatggg cgtctgagca tcagtccacg ctgagcgcag gatatcttca
     4201 tgccagtacg gacgctcccg gcagcgatga tctgaacggg attaacgtga aataccgtta
     4261 tgagtttacg gacacactgg ggctgattac gtccttcagt tatgccaacg ccgaagatga
     4321 gcaaaaaacg cattacagcg atacccgctg gcatgaggat tacgtgcgta accgctggtt
     4381 cagcgtgatg gcagggccgt ctgtgcgtgt gaatgaatgg ttcagtgcgt atgcgatggc
     4441 gggtgtggct tacagccgtg tgtcgacttt ttccggggat tatttccgcg taactgacaa
     4501 caagaggaaa acgcacgacg tgctgaccgg aagtgatgac gctcgctaca gcaacacatc
     4561 tctggcgtgg ggggctggcg tgcagtttaa cccgaccgaa tcagtggccg ttgatgtcgc
     4621 ttatgaaggc tccggcagcg gtgactggcg cactgacggt ttcatcgtgg gtgtcggtta
     4681 taaattttga ttagtaacac agcgttatga cagcccgccg gttcaggcgg gcttttttgt
     4741 ggagtgggta tggcagcagt acaaatatca ggcgtgctga aagatggtgc gggaaaacca
     4801 atacagaact gcaccattca actgaaagcc agacgtaaca gcaccacggt ggtggtgaac
     4861 acggtggcct ctgaaaatcc ggatgaggca gggcgttaca gcatggacgt cgagtatggt
     4921 cagtacagcg tcactctgtt ggtggaggga ttcccgccat cacatgccgg gaccatcacc
     4981 gtgtatgaag attctcaacc ggggacgctg aatgattttc tcggtgccat gtcggaggat
     5041 gacgtccggc cggaggcact gcgtcgtttt gaactgatgg tggaagaagc ggcgcgtcac
     5101 gctgaggagg cgaagaagaa tgccggagag gcggagacgt ccgcgaggaa tgccggcata
     5161 tcagccagtc aggcagaaga gagcgcggca aatgctgaca cttcagcagg ggatgcatcg
     5221 gagtcagccc ggcaggcggc agaaagtgca gccgctgcaa agcagtcaga ggaggcgtcc
     5281 tcgtcctcgg cttctgc
//
LOCUS       DBFIFM010000254         5251 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.254, whole genome shotgun sequence.
ACCESSION   DBFIFM010000254 DBFIFM010000000
VERSION     DBFIFM010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.254"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            73..1686
                     /gene="mppA"
                     /locus_tag="N7C74_004331"
     CDS             73..1686
                     /gene="mppA"
                     /locus_tag="N7C74_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HGU2434071.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVCSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDVHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1737..2768)
                     /gene="ynaI"
                     /locus_tag="N7C74_004332"
     CDS             complement(1737..2768)
                     /gene="ynaI"
                     /locus_tag="N7C74_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HGU2434072.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            3012..3269
                     /locus_tag="N7C74_004333"
     CDS             3012..3269
                     /locus_tag="N7C74_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HGU2434073.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(3319..4269)
                     /gene="uspE"
                     /locus_tag="N7C74_004334"
     CDS             complement(3319..4269)
                     /gene="uspE"
                     /locus_tag="N7C74_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HGU2434074.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(4421..5173)
                     /gene="fnr"
                     /locus_tag="N7C74_004335"
     CDS             complement(4421..5173)
                     /gene="fnr"
                     /locus_tag="N7C74_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HGU2434075.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDTLAQLAGHTRNVA"
BASE COUNT         1429 a         1201 c         1261 g         1360 t
ORIGIN      
        1 aagttgtttg catttcttta aggcgaaaca aataattacg catcaatttt aatgtcggtt
       61 agagggaaac ttatgaagca ctctgtttca gtcacgtgtt gtgcgctgtt ggtcagcagc
      121 atttctcttt cgtatgctgc agaagttccg agcggcacag tactggcaga gaagcaggag
      181 ctggtgcgcc acattaaaga tgagcctgcg tcgctggatc ccgctaaagc cgtgggcctg
      241 ccagagattc aggtcattcg cgatctgttt gaaggactgg tgaatcagaa cgagaaaggg
      301 gagattgtcc ccggcgttgc gactcagtgg aaaagtaatg acaaccgtat ctggactttt
      361 accctgcgcg ataacgcaaa atgggcggat ggcacaccgg taacggcgca agattttgtc
      421 tgcagctggc agcgtctggt ggacccgaaa acgctatcgc catttgcatg gtttgccgcg
      481 ctggcgggaa tcaacaacgc acaggcgatt attgatggta aagctacacc tgaccagctt
      541 ggcgtcaccg cagttgatgt ccatactttg aaaattcagc ttgataaacc gttgccgtgg
      601 tttgtgaatt taaccgctaa ctttgccttc ttcccggtgc aaaaagccaa cgtagaaagc
      661 ggtaaagagt ggacgaaacc cggaaatctg atcggcaatg gcgcttatgt tcttaaagag
      721 cgcgtagtca atgaaaaact ggtcgtggta ccgaataccc attattggga taacgccaaa
      781 acggtactgc aaaaagtgac cttcctgcca attaatcagg aatccgcagc cactaagcgt
      841 tacctcgcgg gggatattga tatcaccgaa tccttcccga aaaatatgta tcagaagctg
      901 ttgaaggata ttccggggca ggtttatacg ccgccgcagc tcgggaccta ttattatgcg
      961 tttaacacgc aaaaagggcc gacggcagat cagcgcgttc gtctggcatt aagtatgacg
     1021 atagatcgcc gcctgatgac cgaaaaagta ttagggacgg gcgaaaagcc agcgtggcat
     1081 tttacaccag atgttaccgc cggatttacg ccggaacctt cgccgtttga acaaatgagt
     1141 caggaagaac tgaatgcgca ggcaaaaact ttgttgagcg cagctggtta tggtccgcaa
     1201 aaaccgctga agctgacgct tttgtataac acttcagaaa accatcaaaa aattgcgatt
     1261 gctgtagcat cgatgtggaa aaagaacctt ggcgtagatg ttaaattgca aaatcaggaa
     1321 tggaaaacct atatcgatag ccgtaacacc ggcaattttg atgttatccg cgcctcatgg
     1381 gtgggggatt ataatgaacc ctccactttc ctgacattat taacgtcaac gcattcagga
     1441 aatatttcac gctttaacaa tccggcatac gacaaagttc tggcccaggc atcgacggaa
     1501 aataccgtta aagcgcgtaa tgccgattac aacgcggcag aaaaaatcct catggagcaa
     1561 gcaccgattg caccaattta tcaatatacc aatggacgat taatcaaacc gtggctgaaa
     1621 ggttatccca ttaataaccc tgaggatgtg gcgtacagtc ggactatgta tattgtgaag
     1681 cattgatgtg atgggaactg gcgttaccct tgtggataac gccagtgatg tctgatttag
     1741 cgaccctgtt ccggcggtgt aatgttatcc atatacagcg tctggctggg gaaggcaaag
     1801 tccgcgccgt gtgactgtac aatatcgata atcttcaaat aaacgtcttg ctgtgcagca
     1861 agccactcag cccataccgt ggttttggta aagcaataaa ccataatatt caatgacgag
     1921 tcagcaaact ggttgaaata aaccagtaag gtttgtcgct ggtcgatggc cgggtgattt
     1981 ttcagcatct cacgtacggc ttcgacgata acgcccactt ttgccgcatc ctcataacgt
     2041 aaaccaatgg tcgtggtaat gcggcggttg gtcattcgtc ctgggttttc tacgctgatc
     2101 gatgaaaaca gcgagttcgg tacgtacaat ggacgattat caaaggtcgt aattttggta
     2161 attcgccagc caatttccgc tactgtacct tcgatatttc tgtccggtga acggatccag
     2221 tcgccaatac tgaaaggacg gtcgaaatag agcatgatcc cggaaaagaa attactcaga
     2281 atatctttgc cagccatacc gacagccaga ccaccaatac caccaaaggt cagcaagcca
     2341 gaaaggctca tgccgaaatg ttcgccataa agcagaacaa gcaccacaat aatggtgatt
     2401 ttgataatgc gcgacataat ccgcgcactg gtgatatcgc gacccttttt aatctgctgt
     2461 ttttcaaact gattaatcag caaaaatagc ttaatcgtca gaataaccgc aatcagggac
     2521 gtacagataa aatcgataac gcctggagtg ataaatttga gtttatagtt ttctataaca
     2581 taattaataa tgctaccaac agcactgata attatggtgt agattaaaaa ttgcaccgca
     2641 tggaataaaa agccttttct tttacgattt ccgcggcgaa accaaaagct catcagaatc
     2701 aatgctgcgc agctaccgaa aataatgacc agattaagcg cattatttgt aaacagttca
     2761 gcgatcattg ttttatcagg ctcctccaga taattgtcgt catgccggaa acccctggcg
     2821 gggctatttt accgcgacaa ttcattcaga tcatcaatag tcagggaagg aagtagcaac
     2881 attagctaac taatcaatgc gttgattgta aatccagcta agaggtgagg ttttcagagc
     2941 agacaatggt gaaatgtcat ggtattgtta cgtttaggta acaagaaatt tgtctgcaca
     3001 aggattacat catgattatg gcgaaactga agtcagcgaa agggaagaaa tttctctttg
     3061 gtttgttggc ggttttcatt attgcggcgt cggttgtgac tcgcgcgact atcggcggcg
     3121 ttatagaaca gtacaatatt ccgctgtcgg agtggacgac atcaatgtat gtgattcagt
     3181 catcgatgat ttttgtttat agcctggtct ttactgtgtt gctggcaatc ccgttgggaa
     3241 tttatttcct tggcggcgaa gagcagtaag taaaaaatag gcccgataac tcgggccttg
     3301 tcagttattg aggagtcgtt aatcgtcttc ttcgtcatcc agttcaacgg gtgtctgata
     3361 ctggtcaggt ttaatgacca acagatcgca gcgaagatga tcaatcacct gttccgccgt
     3421 gttgccgagg aatgctgctg aaataccggt gcgtcctacc gtgcccagaa ccacaatccc
     3481 cgcctgtaag tgctccgcca aatcaggaat cacctcttct ggcagacctt tttctacgtg
     3541 cgtcatgttt tcattaatgc cgaatttctg ccgcagggct ttcattgcca gcaaatgttg
     3601 cccacgaatg gcatcgttat aaacgctcgg atcaaattcc ggcagttcaa tcgcgatatt
     3661 aattggcgtt accggataag cgccaaccag atgaacttcg gtatggttga cttgttctgc
     3721 cagttcgatc gtctctttga ccagtttttc attgagcgca ttatgatacg gctcttcact
     3781 ggcgagattc accgccacca gcgccttacc tccttccggc cacggctggt ctttcaccat
     3841 ccacaccggg cttgggcatt tgcgtaacag gtgccagtcc gttggcgtaa aaatcaccgc
     3901 ttccagacgg tcatgttggt gcgccatttt tagcaccaaa tcgtgcccgc cgctgatcac
     3961 ttcctgaatg atggcttcga aaggacggtt atgccagacc actttaattt caatgggaac
     4021 gccagcattg agataatatt ttgcctgctc gtggatccag gctgtacgct ggctgatgac
     4081 gccctgacgc atagcggtac gttcgtccgg ggagagcagg gtggtcattt cgtatgagaa
     4141 gtcatagatc ggcaaaaagg ctttaatttt gccaccaatc cgttgatgta aataaacagc
     4201 tcgccgcaat gctggttggt cgtcctggtt aggatcgata acaacgagca tgttctgata
     4261 catagccata cagggtctcc ttacaacaac tgtcaacgca gtttgtaatt aaaagattaa
     4321 cccatatctg gtgaatgaaa cagtgatgaa ccttctgcca gatcaataaa tcagaaaaat
     4381 ttaatgatat gacagaagga tagtgagtta tgcggaaaaa tcaggcaacg ttacgcgtat
     4441 gaccagcaag ctgggccagc gtatcgttat tttcgatggt gatgtattta cctttgactg
     4501 ccagcatacc gcttttctgg aagcgaccca gcagacggct gatggtttct accgtcaggc
     4561 ccagatagtt accgatatcg ccacgagtca tcgtcaggcg gaattcacga ggggagaagc
     4621 cgcgttgggc aaaacgacgg gacaggttgt agatgaatgc agccagacgt tcctcggcat
     4681 ttttctttga caacagcagg atcatgtcct gatcgccttt gatttcaccg ctcatcagac
     4741 gcatcatctg ctgacgcaga ttcggcattt taccggacaa atcgtccagc gtttcgaacg
     4801 ggatttcaca taccatcgag gtttccagcg cctgcgcgaa gctcgggtga tggccgctgc
     4861 cgatggcgtc aaatcccacc aggtcgcctg ctaaatggaa accagtgatt tgctcgtcgc
     4921 cttgctcagt gatggtataa cttttaatcg taccggagcg gatggcataa agcgatttaa
     4981 gttcatcacc agccttaaac agcgtctggc ctttctgaat aggcttcttc cgctcaatga
     5041 tattatcaag ctgatcaagc tcatgttcgt tgagtgtgaa cgggatgcaa agctggctga
     5101 tgctgcaatc ctggcaatgg atagcacaac cgccagactg aatgcgccgt ataattcgct
     5161 tttccgggat cataggtctg ctcaagccgt aattgatatt tgtcaatttt aacatctttt
     5221 tagggagcaa gtaagtctaa gcaaacctta a
//
LOCUS       DBFIFM010000255         5226 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.255, whole genome shotgun sequence.
ACCESSION   DBFIFM010000255 DBFIFM010000000
VERSION     DBFIFM010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.255"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(276..2267)
                     /gene="tkt"
                     /locus_tag="N7C74_004336"
     CDS             complement(276..2267)
                     /gene="tkt"
                     /locus_tag="N7C74_004336"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGU2434076.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2545..3303
                     /gene="loiP"
                     /locus_tag="N7C74_004337"
                     /gene_synonym="yggG"
     CDS             2545..3303
                     /gene="loiP"
                     /locus_tag="N7C74_004337"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HGU2434077.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(3509..4429)
                     /gene="speB"
                     /locus_tag="N7C74_004338"
     CDS             complement(3509..4429)
                     /gene="speB"
                     /locus_tag="N7C74_004338"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HGU2434078.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(4565..>5226)
                     /locus_tag="N7C74_004339"
     CDS             complement(4565..>5226)
                     /locus_tag="N7C74_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434079.1"
                     /translation="QYNSSASGNVGTAWGGDVHSTVQGVSAERAWRDPAEMIVISYST
                     NVPSGYDRVYSIRINELEYAIRDGNFNSLPITRVYDSSNNEPRYIVHARVGMNYQLYV
                     RNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYIVNAGDSLAIKGFRKDKHTEAAFQF
                     ANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQNALPPCSGQAFPADNNGYAPPPCR
                     K"
BASE COUNT         1268 a         1379 c         1371 g         1208 t
ORIGIN      
        1 gtaaaaataa aatactcgtg cggtttaatg aaagcaattg agcagggaaa ccggtgtttt
       61 gaaaacagtg aattattaat ttcttatata acattgagtt atagatataa caacaaatgt
      121 caacacgcat atcgtgatgc atattacata attgatattt attgcatata aatatttgcg
      181 atctacaaca cgctttatct attgcttgtc cgcaaacgga catatcaagg taataaaaaa
      241 ggtcgccgaa gcgacctttt tacccgaaat gctagttaca gcagttcttt tgctttcgca
      301 acgacgttgt cgacggtgaa gccgaactct tcgaacagca actctgccgg agctgactca
      361 ccgaaggtgg tcataccgat gatagcgcca ttcaggccaa catacttgta ccagtagtca
      421 gcaatacccg cttctaccgc aacgcgtgca gtaaccgctt tcggcagtac ggattcacgg
      481 taagccgcat cctgcttgtc gaatgcatcg gtagacggca tggaaaccac gcgcgccttc
      541 acgccttcgg cagtcagttt ttcgtaggcg gcaacagcca gttcaacttc cgaaccggtc
      601 gcgatgaaga tcagttccgg ctgaccagca cagtctttca gcacataacc accgcgcgcg
      661 atgtttgcca gttgctcttc agtacgttcc tgctgcgcca ggttctgacg ggagaggata
      721 agcgcggtcg gaccgtcctg acgctcaaca ccgtatttcc acgcgaccgc ggattcaacc
      781 tggtcacacg gacgccatgt agacatgttc ggggtcacgc gcagagaagc aacctgctca
      841 accggctggt gagtcgggcc atcttcgccc agaccgatgg agtcgtgggt gtaaaccata
      901 acctgacgct gtttcatcag cgcagccata cgtacggcgt tacgtgcgta ttcgacaaac
      961 atcaggaagg tggaggtgta cggcaggaag ccaccgtgca gggagatacc gttagcaatc
     1021 gcggtcatac cgaactcgcg aacaccgtag tggatgtagt tacccgcagc atcttcgttg
     1081 attgctttag aaccagacca cagggtcagg ttagacggtg ccaggtcagc ggagccgccg
     1141 aggaattccg gcaacagcgg gccgaacgct tcgatagcat tctgagacgc tttacggctg
     1201 gcgattttcg ccggattagc ctgcagttta gcgataaact ctttcgcttt ggcgtcgaag
     1261 tcagacggca tttcgccttt catacggcgg gtaaattcag ccgcttcctg cggataagct
     1321 ttcgcgtaag ccgcaaactt ctcattccat gcagattctt tcgcctggcc tgcttctttc
     1381 gcatcccact gcgcatagat ttcagacggg atttcgaacg gtgcgtattt ccagcccagc
     1441 tgttcgcggg tcagagcgat ttctgcatca cccagcggcg caccgtggga gtcgtgagta
     1501 ccggctttgt tcggggaacc gaaaccgatg atggttttgc acatcagcag ggacggtttg
     1561 tcagtcactg cgcgcgcttc ttctactgcg cgtttgatgg atgctgcgtc atgaccgtcg
     1621 atgtcgcgaa taacgtgcca gccgtaagct tcgaaacgca ttgcggtgtc gtcggtgaac
     1681 cagccttcaa cgtgaccatc gatagaaata ccgttgtcat cgtagaacgc aatcagttta
     1741 cccagcttca gcgtacccgc cagagagcaa acttcgtggg agatgccttc catcatgcag
     1801 ccgtcgccca tgaaggcgta ggtgtagtgg tcgacaatgt cgtggcccgg acggttaaac
     1861 tgcgccgcca gcgttttttc tgcaatcgcc atacctactg cgttggcaat accctgcccc
     1921 agcggaccgg tggtggtttc cacaccagcg gtgtaaccca cttccgggtg gcccggagtt
     1981 ttagagtgca gctgacggaa gtttttcagt tcttccatcg gcagatcgta accggtgagg
     2041 tgcagcaggc tgtagatcag catggagccg tggccgttgg acagcacgaa gcggtcacgg
     2101 tcagcccagg acggattctg cgggttatgt ttcaggaaat cacgccacag gacttcggca
     2161 atgtcagcca tacccatagg ggcacccggg tgaccggatt tggctttctg tactgcgtcc
     2221 atgctcagcg cacgaatagc attggcaagc tctttacgtg aggacatttt gactccagat
     2281 cggatgatga agggcacgcc cttaacgact tgacgacagc gcgttttggg ctacgccgga
     2341 aaatttgcca acaatttacc gcaagccgcg cgtcatgtac atggaacatc cttttgccgc
     2401 ttcagaaatc tctggatcat gctcgcatgt tgcgcaatct actcgcccgt ccgctgcgct
     2461 tttccttata ctgagactga gcgtcggttc acctgcaaac ggcgcatttt tagaataatc
     2521 ctgaccttgt gcggaagaaa aaacatgaaa attcgcgcct tattggtagc aatgagcgtg
     2581 gcaacggtac tgaccggttg ccagaatatg gactccaacg gactgctctc atcaggagcg
     2641 gaagcttttc aggcttacag tttgagtgat gcgcaggtga aaaccctgag cgatcaggca
     2701 tgtcaggaga tggacagcaa ggcgacgatt gcgccagcca atagcgaata cgctaaacgt
     2761 ctgacaacta ttgccaatgc gctaggcaac aatatcaacg gtcagccggt aaattacaaa
     2821 gtgtatatgg cgaaggatgt gaacgccttt gcaatggcta acggctgtat ccgtgtctat
     2881 agcgggctga tggatatgat gacggataac gaagtcgaag cggtgatcgg tcacgaaatg
     2941 gggcacgtgg cattaggcca tgtgaaaaaa ggaatgcagg tggcacttgg tacaaatgcc
     3001 gtacgagtag ctgcggcctc ggcgggcggg attgtcggca gtttatctca atcacaactc
     3061 ggtgatctgg gcgagaaatt agttaattcg caattctccc agcgccagga agcagaagcc
     3121 gatgattatt cttacgatct tctgcgccaa cgcggcatca gcccggcagg tcttgccacc
     3181 agctttgaaa aactggcaaa actggaagaa ggtcgccaaa gctcaatgtt tgacgaccat
     3241 cctgcatccg ccgaacgcgc ccagcatatt cgcgatcgca tgagcgcgga tgggattaag
     3301 taaagcctgg tggtgtcgaa cgtaggtcag ataaggcgtt cacgccgcat ctgacatgaa
     3361 tggcacattt gttaccttgt gcgcattgcc ggatgcgatg ctggcgcatc ttatccggcc
     3421 tacgggtgcc gaacgtaggt cggataaggc gttcacgccg catccgacat taatggcacg
     3481 ttttacccgt gcgcatcgca tctggtgctt actcgccctt tttcgccgcc tgaatataca
     3541 gcatttccag cgccagcgtt gccgctgcca gagcggtgat ttccgactga tcgtatgccg
     3601 gagccacttc cactacgtcc atcccaacga tgttgagatc tttcaggccg cgtaccagtt
     3661 taatagcgcg atcggaggtc agaccgccaa ttaccggcgt accggttccc ggcgcaaatg
     3721 caggatccag gcagtcgata tcaaaggtca ggtaaaccgg catatcaccc acaatttgtt
     3781 tcacctgggc gataacgtca tccacgctgc gatcgttcac ctggcaggcg tccagcacgg
     3841 taaagccgtt gtctttatca aattcggtac gaataccaat ctgcacggaa tgattcgggt
     3901 cgatcagacc ttctttcggc gcggtataga acatggtgcc gtggtcaaat tcacaaccgt
     3961 tcgcgtaggt atcggtgtgg gcgtcaaagt gtaccagcgc cattttgccg aaatgcttcg
     4021 catgagcacg cagcagcggc agcgtaacaa agtggtcacc accgaaggag agcatacgct
     4081 taccggcagc cagcagcttc tcggcgtgcg cctgcagctt ctcgctcatc tcacgggcat
     4141 cgccgaaagc atataccaga tcaccacagt ccacgacgtt cagacgctca cgcatgtcga
     4201 aattccacgg gaagcggttg tgttcccagg ccagattcgt cgaaacctga cggatagctg
     4261 ccggaccgtg gcgtcccccc gcacgaccag aagtggccat atcgaacggc acaccagtaa
     4321 tcacccagtc agcatcgctg tcatacggct ggaagttcat cggcaggcgc aaaaaaccaa
     4381 aggcgttgga aaccagtgag ttatcgtatt gatgacctaa ggtgctcatg gatggactcc
     4441 tattacaaag tcgatataaa aaaacccttc cgcgtcgtta ggcccgacga ggaagggttg
     4501 gatttgcgaa acacaagttt gtgctaatta tcgccttaaa tgggcgaggc atcaagcgag
     4561 gttgctattt ccgacacggt ggcggcgcat agccattgtt atctgccgga aaggcttgtc
     4621 cagagcacgg tggcagcgcg ttttgcgcct ggccttgtaa ttcaaaggcg gcaaagccga
     4681 ttacaccggt attacgcaca tcgccttgcg ccgaattggc tgcgtaggaa tcagcaacgt
     4741 tggcgaactg aaaagccgct tcggtatgtt tatctttgcg gaatccttta atcgctaacg
     4801 aatctccggc attcacaata tagccgttgt tattcagcga accttgcttg ccgttcagca
     4861 catccaggcc atcaaccgta gcaacgatct cgtagttggt gttacgactg taattacgga
     4921 catataattg ataattcatt ccgactctgg catggacaat atagcgtggt tcattattag
     4981 atgaatcata aacacgcgta ataggtagag aattaaaatt accatcgcga atagcatatt
     5041 ccagttcgtt aatacgaatg ctataaacgc gatcgtagcc ggaaggaaca ttagtcgaat
     5101 aactaataac gatcatctcc gccggatcgc gccaggctcg ctcagcagat acgccctgaa
     5161 ccgttgagtg cacatcccca ccccaggcgg tgccgacatt gcccgatgca ctgctattat
     5221 actgcg
//
LOCUS       DBFIFM010000256         5168 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.256, whole genome shotgun sequence.
ACCESSION   DBFIFM010000256 DBFIFM010000000
VERSION     DBFIFM010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.256"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            91..1149
                     /gene="tsuA"
                     /locus_tag="N7C74_004340"
     CDS             91..1149
                     /gene="tsuA"
                     /locus_tag="N7C74_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HGU2434080.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGAFIAAKASREFRVRA
                     ADAQTTVRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWVGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            1163..1390
                     /gene="tsuB"
                     /locus_tag="N7C74_004341"
     CDS             1163..1390
                     /gene="tsuB"
                     /locus_tag="N7C74_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HGU2434081.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1433..2857)
                     /gene="sbcB"
                     /locus_tag="N7C74_004342"
     CDS             complement(1433..2857)
                     /gene="sbcB"
                     /locus_tag="N7C74_004342"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HGU2434082.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSD
                     TLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQMLA
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            3039..4217
                     /locus_tag="N7C74_004343"
     CDS             3039..4217
                     /locus_tag="N7C74_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2434083.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     DQIKPFLEKAMTTYNDVLFPGEDINKPISIAAANRFVNRIRGGMDLGYWRTHDFRRTL
                     VTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELHADKLFWHIRSISD
                     "
     gene            complement(4198..4389)
                     /locus_tag="N7C74_004344"
     CDS             complement(4198..4389)
                     /locus_tag="N7C74_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2434084.1"
                     /translation="MTDTSLIPEKEVMNKLGVSSRQTIWNYTKRHGFPKPVRTHPKSY
                     LREAVEGWILNGGVNQKCS"
     gene            complement(4467..4811)
                     /locus_tag="N7C74_004345"
     CDS             complement(4467..4811)
                     /locus_tag="N7C74_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434085.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHGFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
BASE COUNT         1272 a         1218 c         1291 g         1387 t
ORIGIN      
        1 tttgttctaa tttagagaat ttggttattg gtctgcattc caccagtcat gaataatttc
       61 cccattaaca ttcatctggt taattaattt atgttttcaa tgatattaag cgggctaatt
      121 tgtggtgctc tgctgggatt tgtcatgcag cgtgggcgtt tttgcctgac tggtggtttt
      181 cgcgatatgt atatcgcgaa aaataatcgc atgttttacg ccttgctgat tgcaatttcg
      241 gtacaaagcg tgggggtttt tgcgttaatt caggcgggcc tactgactta cgaagccggg
      301 gcgttcccgt ggcttggtac tgttataggt gggtatctct tcgggctggg aattgttctg
      361 gcgggtggat gtgccaccgg gacctggtat cgcgcgggtg agggattgat cggcagttgg
      421 atcgcgcttt tcacttatat ggtgatgagt gcggtgatgc gttctccaca cgccagtggt
      481 ttaaatcaaa ccttgcagca ctacactact gaacataact ctattgctga gacttttaat
      541 ttgtctgtgt ggccgttggt tgccgttttg ctggtgataa cgctctgggt ggtgatgaaa
      601 gagttgaaga agccaaaact caaagtcgcg accttaccgc cgcgccgaac cgggatcgct
      661 catattctgt ttgaaaaacg ctggcatccc tttgtcacag ctgtactcat cggtttgatt
      721 gcgcttttag cctggcccct gagtgaagca accggacgta tgtttgggtt aggcatcact
      781 tccccaacgg ccaatattct gcaatttctg gtcgcgggtg acgtgaaata cattaactgg
      841 ggcgttttcc tggtgttagg gatcttcgtg ggggcattta ttgcagccaa agcgagccgt
      901 gagttccgcg ttcgcgcagc tgatgcacaa acaacagtac gtagcgggct aggtggtgta
      961 ctgatgggct tcggtgccag tattgcaggt ggttgctcta tcggtaatgg actggttatg
     1021 actgcaatga tgacctggca gggctgggtt ggccttgtat ttatgattct cggagtctgg
     1081 actgcgtcct ggcttgtgta tgttcgaccg cagcgtaagg cgcgactggc aactgctgcg
     1141 gcaaattaag taaggtgtaa agatggcgat taaaaagctg gatgtggtga cgcaggtctg
     1201 tccgtttcca ctcatagaag caaaagcagc actggcagaa atggccagcg gcgatgagtt
     1261 ggtaattgag tttgattgca cccaggcaac tgaagcaatc ccacagtggg cggcagaaga
     1321 agggcatgcc atcaccgatt atcagcagat tggcgatgca gcgtggagca tcaccgttca
     1381 aaaagcctga ctcaacattg tcctccgccg taccagcggc ggaggcttca cattagacaa
     1441 tctcttccgc gtactgccaa agcgctttta acagcgccac tttttcttta tcgtcggcat
     1501 actgctgcgc cagcatctga atctcttctg catagccttg taaaaactct ggcgtgaaga
     1561 cctggcggcg gtgctccagc cagcgttgct gctcggcata atccagcgtc cccgggaagt
     1621 tgcgtgcccg ataattgaac aacagctttt caatccgttt atcaacaaaa gtgatatcca
     1681 gtgccggtaa attacgcggc tcggtttcaa gcacaatttt cattgctgca cgatcggcgt
     1741 cactgaaaaa gccattataa agctgtgcat ccacgttatc tgaaggcgta aacggttcgg
     1801 cttccgcgaa tatcgccacc actttttcac gcacttgcgg attttcacgc agaattttca
     1861 ggttatcgag gcaatgctga cgattaattc ccagtcggtc ggcatcttcc gggcgtagcg
     1921 tattcgcctg ggccagcacc ggacatttat tgatatgtac cagtttaacc ggaacggcgg
     1981 cgttatcgcc aagatcggtt tttgcggtat ataaacgctc gcgcaatgtg tcgctatcca
     2041 gttccagtaa tggcgaaata tctcctgcca aatccaccat aattacggca ttgcgatttt
     2101 caggatgcca cgccagcggt gccacccagc tggtattgcc gcgccatgcc ccaaacattc
     2161 cggaaacgtg aaccaggggt ttcatctgcg gaacatcaat caacgccatc agtttgtgtt
     2221 tattacgatg ggtaaagaga taatcaaaca ggcgtggttg acgcgttttt accagtttcg
     2281 ccatcgcaat agtggcgtac acatcagcca tcgcatcgtg ggcgttgcta tgttcaatac
     2341 cattcgcttt ggttaaatgc tcaaggcgaa agctcggtag accgtcatca ttttcaggcc
     2401 agtttattcc ttccgggcgc agggcatagc aggcacgcat aacatccagt aaatcccagc
     2461 gcgagttatc atgctgccag ctccaggcgt aaggatcgta gaaattacga taaaaaacgt
     2521 tgcgtgtgac ttcgtcgtcg aaacgcacat tgttgtagcc cagaatacag gtcttcggta
     2581 cggtaaaaag cgagtgaata cgggcggcaa atgcggcttc gttttctcct ttcgcccgag
     2641 cttcctgcgg ggtaataccg gtaattaata ctgctccagg ctggggtaaa tagtcatccg
     2701 cgggcttgca gtaaaagact tcgggttcgc cgatgacatt gaattcgtca tcggtgcgaa
     2761 tggctgcgaa ctgtgcaggg cgatctaacg cggggtgcgt gccaaaggtt tcgtaatcgt
     2821 gaaagaggaa ggtaggttgc ttatctgtgt ctgtcattgt ctagcattgt ccaatgtagt
     2881 taattaactg tctgttataa ctcgaaaaac aacccttttt tcacctgagt atgtctacga
     2941 acgtcaaata tagttgttta ctgtgcgctg ttgtccatgt gatattgtgt accagatgtg
     3001 taccaaacca ttatttctga gcgtaccaaa tattatttat ggctataagc gacacaaaac
     3061 tgcgtactat ttatggtaaa ccatattctg gcccacaaga agtggctgat gccgatggcc
     3121 tcagcgtacg aatttcaccg aagggggtca tccagttcca gtaccgctat cgctggcatg
     3181 gcaagcctaa tcgacttggg cttggtcgat acccatccct gtctttgaag gatgccagac
     3241 agatcactgc tgacttgcga aagctctatt tctcaggaac ggatccacgc acttattttg
     3301 aagagaaggt ggagaactcc atgacggtcg cccagtgtct cgactactgg ttcgacaact
     3361 acgtctctac aactctcaga gaaaagaccc aagcacttta ccgatcaacg gttatgaagc
     3421 gcatgcatga cgccttccct aatcgtccgg catcttctat cacggttaag caatgggttg
     3481 acctgcttac cgaagaagaa aaagataatc cacgccgagc aaggcaggtg ctaagtcaac
     3541 taagatcagc aattagttgg tgcatgcggc gtcagttgat agatagttgc gcaattatga
     3601 gcatccaacc aagggacttc ggctcccgcg ctgaggtagg ggatcgggta ctgtcgtatc
     3661 acgaactggc taagatctgg cttgctattg aaagaagccg tgcgtctacg tcaaataagc
     3721 tccttcatca gatgcttatg ctgtgggggg cgaggctctc agagcttagg ctggcaacaa
     3781 agacagaatt tgacctgctg gacaacgtat ggaccgtacc gaaagagcat agcaagatgg
     3841 gtaatgttat ccgccgtcca atcttcgatc aaattaagcc ttttctcgaa aaggccatga
     3901 caacgtacaa tgatgttctt ttccctggag aagacataaa caaaccgatc agcatcgctg
     3961 cagctaaccg attcgtaaat agaataaggg gagggatgga tctaggttac tggcgaacac
     4021 atgatttcag aagaacgctt gttacacgtc tgtccgagat gaatgtcgag cctcatgtta
     4081 ctgagcgaat gctcggtcat gaacttggcg ggataatgtc cgtatacaat aaacacgact
     4141 ggatagaggc tcagcgcaaa gcgtatgagc ttcacgctga taaattgttc tggcacatca
     4201 ggagcatttc tgattaacgc caccgttaag aatccaccct tcaacagctt cacgaaggta
     4261 tgatttgggg tgggttctga ctggcttcgg aaatccgtgc cgtttggtat agttccagat
     4321 tgtctgacgt gatgaaacac cgagcttgtt catcacttct ttctcaggaa tcaggctggt
     4381 atcggtcatc ttaattctcc aggcaaaaag aaaccgccat atagcggctc tatcagatat
     4441 gaacaggcct catcgagtgt gaggcgttag tccttgcgta gctcgctgat tcttctgtaa
     4501 gtctctggtg ctttgtttcc gtgtatcttc atttcagact tcaacagagc aacgagggaa
     4561 tcccattcgt tgaggattcc tttgaatgcc ggaacgcgct ttgcaacctt gtcgaatgaa
     4621 tctctgattt ctggaatctg ctcaacaagt gcaacgcatc gtctgaaatc ggctgcgtca
     4681 tgtggagcac cgaagccatg accatagata ttctttttca ggccacatgc gattgaggca
     4741 agagttgcgc tactgatgcc gacatcgcca gttgattgcc atttcaaaac cttcatagcc
     4801 aaatctgaca tttcttgtct ccataaaaca aaactcgccg tagcgagttc agataaaaga
     4861 aatccccgcg agtgcgagga ttgttagttg cgctctgctg ctgccttagc cattaccata
     4921 tccacacaat ttcaccagac atgattctcg caatcactat catcaccaaa gtgataatca
     4981 caactttaac tggcggcatc attcaccatc ctgctgcggt gctgctggat atacttttct
     5041 catccaatgc gtggcgtcat gaataacgaa ctgatattca tcatcagtaa acatcgagcc
     5101 atcccactgt agcccgtcaa gaacacctct ggatggagag aatacccaga tatcgaaatc
     5161 gtatttct
//
LOCUS       DBFIFM010000257         5092 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.257, whole genome shotgun sequence.
ACCESSION   DBFIFM010000257 DBFIFM010000000
VERSION     DBFIFM010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.257"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            19..3921
                     /gene="espP"
                     /locus_tag="N7C74_004346"
     CDS             19..3921
                     /gene="espP"
                     /locus_tag="N7C74_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspP"
                     /protein_id="HGU2434086.1"
                     /translation="MNKIYSLKYSHITGGLIAVSELSGRVSSRATGKKKHKRILALCF
                     LGLLQSSYSFASQMDISNFYIRDYMDFAQNKGIFQAGATNIEIVKKDGSTLKLPEVPF
                     PDFSPVANKGSTTSIGGAYSITATHNTKNHHSVATQNWGNSTYKQTDWNTSHPDFAVS
                     RLDKFVVETRGATEGADISLSKQQALERYGVNYKGEKKLIAFRAGSGVVSVKKNGRIT
                     PFNEVSYKPEMLNGSFVHIDDWSGWLILTNNQFDEFNNIASQGDSGSALFVYDNQKKK
                     WVVAGTVWGIYNYANGKNHAAYSKWNQTTIDNLKNKYSYNVDMSGAQVATIENGKLTG
                     TGSDTTDIKNKDLIFTGGGDILLKSSFDNGAGGLVFNDKKTYRVNGDDFTFKGAGVDT
                     RNGSTVEWNIRYDNKDNLHKIGDGTLDVRKTQNTNLKTGEGLVILGAEKTFNNIYITS
                     GDGTVRLNAENALSGGEYNGIFFAKNGGTLDLNGYNQSFNKIAATDSGAVITNTSTKK
                     SILSLNNTADYIYHGNINGNLDVLQHHETKKENRRLILDGGVDTTNDISLRNTQLSMQ
                     GHATEHAIYRDGAFSCSLPAPMRFLCGSDYVAGMQNTEADAVKQNGNAYKTNNAVSDL
                     SQPDWETGTFRFGTLHLENSDFSVGRNANVIGDIQASKSNITIGDTTAYIDLHAGKNI
                     TGDGFGFRQNIVRGNSQGETLFTGGITAEDSTIVIKDKAKALFSNYVYLLNTKATIEN
                     GADVTTQSGMFSTSDISISGNLSMTGNPDKDNKFEPSIYLNDASYLLTDDSARLVAKN
                     KASVVGDIHSTKSASIMFGHDESDLSQLSDRTSKGLALGLLGGFDVSYRGSVNAPSAS
                     ATMNNTWWQLTGDSALKTLKSTNSMVYFTDSANNKKFHTLTVDELATSNSAYAMRTNL
                     SESDKLEVKKHLSGENNILLVDFLQKPTPEKQLNIELVSAPKDTNENVFKASKQTIGF
                     SDVTPVITTRETDDKITWSLTGYNTVANKEATRNAAALFSVDYKAFLNEVNNLNKRMG
                     DLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFTVTHTD
                     SSASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYST
                     HSWYAGAEAGYRYHVTEDAWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDYNPLIG
                     RTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSV
                     GLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            complement(4177..4658)
                     /locus_tag="N7C74_004347"
                     /pseudo
     CDS             complement(4177..4658)
                     /locus_tag="N7C74_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1529 a         1117 c         1158 g         1288 t
ORIGIN      
        1 cttaaaaatg gagcttatat gaataaaata tactctctta aatacagcca tattacagga
       61 gggttaatcg ctgtttctga attatccggc agagtatcat caagagcaac tggtaagaaa
      121 aaacacaaac gcatacttgc attatgtttt ttaggcttat tacaatcctc atattctttt
      181 gcgtcacaga tggatatttc aaatttctac atccgtgact atatggattt tgcacagaac
      241 aagggcatat ttcaggctgg cgcaacaaat attgaaatag tgaagaaaga tggctccacc
      301 ctgaaactac cggaagtacc atttcctgac ttctcaccgg ttgcaaacaa agggtcaacc
      361 acatctattg gtggtgcata cagtatcaca gccacacaca atacgaaaaa ccaccactca
      421 gttgcgacgc aaaactgggg gaacagcacg tacaaacaaa ctgactggaa tacttcacat
      481 cctgattttg cagtatcccg acttgacaag tttgttgttg agacccgagg tgcgactgaa
      541 ggcgcagata tttcgttatc aaaacagcag gcacttgaac gttacggggt taattataaa
      601 ggagaaaaga aacttatcgc attcagagcc ggctctggtg tggtatccgt taaaaaaaat
      661 ggacgcataa ctccatttaa tgaggtttct tataagccag aaatgttaaa tggctctttc
      721 gttcacattg atgactggag tggatggctg atattaacca acaaccagtt tgatgagttt
      781 aataacattg cctctcaggg tgacagcggt tcagcactgt tcgtctatga taaccaaaag
      841 aaaaagtggg ttgtcgctgg aactgtctgg gggatttata attacgccaa tggcaaaaac
      901 cacgcagcat acagtaaatg gaaccagaca accattgaca acctgaagaa caagtattct
      961 tacaacgtgg atatgtcagg ggctcaggtt gcaaccattg aaaatggaaa actgacaggc
     1021 actggctcag acaccaccga tataaaaaat aaggacttaa tatttactgg cggtggagat
     1081 atcctcctga aatcctcttt tgataatggt gctggcggtc ttgtctttaa tgataaaaag
     1141 acctatcgag taaacgggga tgatttcacc tttaaaggtg ccggtgttga tacaagaaac
     1201 ggcagcaccg ttgagtggaa tatccggtat gataataaag acaaccttca caaaattggt
     1261 gatggcacat tagatgtccg aaaaacccag aacaccaacc tgaaaacagg tgagggtctt
     1321 gtcattcttg gagctgaaaa aacattcaat aatatctaca taaccagtgg tgatggaact
     1381 gtccgactga atgcagaaaa tgcactgtct ggcggtgaat acaacggtat tttctttgcg
     1441 aaaaatggcg gaactcttga cctgaacgga tataatcagt ctttcaataa aattgctgca
     1501 actgattcag gtgctgtaat aaccaatacg tcaaccaaaa aatccatttt atccctgaat
     1561 aatactgctg actatatcta tcacggcaac ataaacggga atctggacgt acttcagcat
     1621 catgagacga aaaaagagaa ccgtcgtctt attcttgatg ggggcgtgga cacaacaaat
     1681 gatataagcc tgcgtaatac acaactgtcc atgcagggac atgccactga acatgccatt
     1741 tatcgggatg gagctttctc ttgttcacta ccagctccta tgcgcttttt gtgtggcagt
     1801 gattatgttg caggaatgca aaatacagaa gctgatgctg taaaacaaaa cggaaatgcc
     1861 tataaaacca acaatgctgt ctctgattta tcgcagccag actgggaaac cggaacattc
     1921 agatttggaa cgctacatct tgaaaattcc gatttttctg ttggtcgtaa tgcaaatgta
     1981 atcggggaca ttcaggccag taaatcaaac attactattg gtgacactac agcatatatt
     2041 gatttgcatg ctggtaaaaa tattaccggt gatggttttg gcttccgcca gaatattgtg
     2101 cgtggaaact cacaaggaga aacgctgttt acaggaggga tcacagcaga agacagcact
     2161 atcgttatta aagataaagc aaaagcatta ttttcaaatt atgtatacct gctgaacaca
     2221 aaagcaacca tagagaacgg tgctgatgtg acaactcaaa gtggtatgtt ctccacgagc
     2281 gatatcagca tctctggtaa tctgtccatg acaggcaatc ccgacaaaga caataaattc
     2341 gagccctcaa tatatctgaa tgatgcttct tatctactga ctgacgactc cgccagactc
     2401 gttgccaaaa ataaagcatc tgtggtggga gatatacact ccactaaaag tgcatccatc
     2461 atgtttggtc atgatgaaag cgacctctcg cagttgtctg acagaacctc aaaagggctt
     2521 gcacttggtc ttttaggtgg ctttgatgtc tcatatcgcg gttcagtcaa tgccccgtca
     2581 gcatctgcca ctatgaacaa cacctggtgg caactaaccg gagattctgc gctgaaaaca
     2641 ctgaaaagta caaacagcat ggtctatttc actgacagcg caaacaataa gaaattccat
     2701 acgctgacgg tcgatgagct ggcaaccagc aacagcgcct atgcgatgcg tacaaacctt
     2761 tctgaatcag acaaactgga ggtcaaaaaa cacttgtctg gtgagaacaa tattttactc
     2821 gttgatttcc ttcagaaacc aacgcctgaa aaacaactga atattgaact ggtaagcgcg
     2881 ccaaaagaca ccaatgaaaa tgtctttaaa gccagtaaac aaaccattgg tttcagtgat
     2941 gtaacgccgg tcattacaac cagggaaacc gatgacaaaa taacatggtc actgacaggc
     3001 tataacacgg tagcaaacaa ggaagcaacc cggaatgccg ccgccctgtt ctctgttgac
     3061 tataaagcgt ttctgaacga ggtcaacaac ctgaacaaac gtatgggtga cctgcgtgat
     3121 atcaacggcg aagccggtgc atgggcacgc atcatgagcg gtaccggctc tgccagtggt
     3181 ggtttcagtg acaactacac gcacgttcag gtcggggtcg acaaaaaaca cgagctggac
     3241 ggactggatt tgtttaccgg tttcactgtc acacacactg acagcagtgc ctccgccgat
     3301 gttttcagtg gtaaaacgaa gtctgtgggg gctggcctgt atgcttccgc catgtttgat
     3361 tccggtgcct atatcgacct gattggcaag tatgttcacc atgataatga gtacactgca
     3421 acctttgccg gactcggaac ccgtgattac agcacgcatt catggtatgc cggtgcagaa
     3481 gcgggctacc gctatcatgt cactgaggat gcctggattg agccacaggc tgagctggtt
     3541 tacggttctg tatccggtaa acagtttgca tggaaggacc agggaatgca tctgtccatg
     3601 aaggacaagg actacaatcc gctgattggc cgaacgggtg tggatgtggg taaatccttc
     3661 tctggtaagg actggaaagt gacagcccgt gccggtctgg gctaccagtt cgacctgctg
     3721 gctaacggcg aaaccgtatt gcgggatgca tctggtgaaa aacgcatcaa aggtgaaaag
     3781 gacagccgta tgctgatgtc cgttggcctg aatgcagaaa tcagggataa cgtccgcttt
     3841 ggactggagt ttgagaaatc cgcctttggt aagtacaacg ttgataatgc tgtcaacgct
     3901 aatttccgtt actcgttctg atactccgta ttcaaaaggg cctgcgggcc ctttacgctc
     3961 agtgcctgcc gccctgatat gtctgagaga catttccggg atgtcagtcg actggtttac
     4021 ttaactgcga tgactaaagc tggcagtaat gccggctttt ttacggccac atacgtcttc
     4081 ctctcatccg gaactgtgat acaaatcctg catacaggat tgaccgccgg ttcctgcaaa
     4141 tggcacattt tcagtaagat agcgaaggtt acatttgtca ctggttaaca taccgatggt
     4201 aaaaggggca agcagcgcca gcaattcatc agcccggggg ccaaaagtgt aggccagaac
     4261 gccaccagtt ttcgtgttgt acgcgtacca gagcctgtgc tgtcacgctt tactgccgat
     4321 aaaactccat tgctcatcaa gttcgcagat gagtgccata tcagcatgag cgaccgggga
     4381 tgacgttatt cttcgtggcg agcgtttttt aaagtccgaa tgacagtatt aatgccgatt
     4441 ttcagcgtac gggcagtatc gcgaactccg gcaccgttga aaggcatttc agtgatttgg
     4501 tcttgaactc ccggtttacg ggcctcgtaa cggtaagtga gctgaaacac tcgatgacag
     4561 tcacggcagc gaaatcgatc atgtcctttt gggttcttcc cgtgacggta aacctgaacg
     4621 gactggcaac gggggcaatg aaggttaact gtgaccatta aagaacctta aaagtgacta
     4681 ttatacccct aattcaacta attggaggca ttaccatgca ttagtgcaag cgccttgtgg
     4741 attcttttgc taatggcgga aacatattgt acaatattgc atttatccct caataaataa
     4801 atgagttatc agattgcaaa aatctgttat caaactaatt caaataaaaa gagattgata
     4861 cgaccaacgt acacaaaaat tttcgatatt cgaactaaat aacagctcct aataccgcta
     4921 cactttagct atccagtctg cgcctcagaa atggacgatg ccgttgaagg actggtgtat
     4981 ggcaatgagc cgctttatta tcgaattcgg tgaccgcttg gacggtcact tcggagaaaa
     5041 ggcatttaca cagcatccta aacaggttca gcataggtga tataccgcct tt
//
LOCUS       DBFIFM010000258         5070 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.258, whole genome shotgun sequence.
ACCESSION   DBFIFM010000258 DBFIFM010000000
VERSION     DBFIFM010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.258"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            1..507
                     /gene="ykgH"
                     /locus_tag="N7C74_004348"
                     /pseudo
     CDS             1..507
                     /gene="ykgH"
                     /locus_tag="N7C74_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
     gene            complement(750..1445)
                     /locus_tag="N7C74_004349"
     CDS             complement(750..1445)
                     /locus_tag="N7C74_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HGU2434087.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAKNISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAERLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1438..2865)
                     /locus_tag="N7C74_004350"
     CDS             complement(1438..2865)
                     /locus_tag="N7C74_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HGU2434088.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGLWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDNVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2876..3595)
                     /locus_tag="N7C74_004351"
     CDS             complement(2876..3595)
                     /locus_tag="N7C74_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HGU2434089.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(4122..4976)
                     /gene="rclR"
                     /locus_tag="N7C74_004352"
     CDS             complement(4122..4976)
                     /gene="rclR"
                     /locus_tag="N7C74_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HGU2434090.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWSVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT         1355 a         1323 c         1068 g         1324 t
ORIGIN      
        1 ataaatcaga caacacccgg catacttgtt aactgtgata gcaataataa ctttgcctgt
       61 cagacaacaa tgactgaaga tgttattcag cgaattacta cattttttca caccagccca
      121 gatgtcaaga acagagaaat aaagctggaa tggtcaggag ataagagaga tttaccaact
      181 gctgaagcgg aaatatctcg cgtgcaggcg tctattatca aatggtatgc gtcagaatac
      241 cataatggca ggtaagttct cgatgagata caaacgcctt cagcaattaa cagtgagctt
      301 tatacaaaaa tgatatacct gaccaggaac tggtcattgt atccgaacgg tgatggctgt
      361 gtaactataa gctcaccaga aataaaaaat aaataccctg ctgccatttg cctggctctg
      421 ggattttttc taagcattgt aatttctgta atgttttgtc ttgtcaaaaa aatggtagat
      481 gaataccaac aaaactctgg gcagtaataa acagcattct gatagagagt gcctgtataa
      541 agctgaagtg acaatgaccg ggaagggaac ccgttttcag gaatggatgc ctgtcttttt
      601 cactgtaaat atcctggcat tagtaatgcc aggatattta cagaagagga gcataaccat
      661 gacggaacgg ggttaagtca ggttgtcctt aacgctattt tcaacctcgt atgccttctt
      721 caggtttatg tccagacttc atacctctct cagcaatcct caataatcag atacaccgct
      781 ttcaccggac catgaacccc gacgactttg ataagctcaa tgtccgccgt tgagctggga
      841 ccactgatga tgttaataca tgacggcatg cgttcaccgg cctgcgcttt ctgatgcaat
      901 ctttctgcga gttgcgctac acgcggcagg atagtgcttt tacgcaggat aaaaagagat
      961 gattccggga gcaggctcaa tgaacgccca cgctcggcgg cggaaaaaag aaccacgcct
     1021 cccgattcgg ttaaaccata ttcagcatac acgacaccca ctttagcctg ctctgcctgc
     1081 gagatattct tggcaccttt cgccggatcc caaacaacgg cattgcattc ctgctgcaaa
     1141 cgttcgctaa tccctaattc ctccagcctc gtgtcaccgc taatcacgac cgactgatct
     1201 cccagctctt tacacagacg tattgcagct tctgccgcct tcgcctcgct ggtcagctca
     1261 cagcgcgtca acataacatc gctggcaaac tgaataaacg cgtcacagcg ctgctgttgg
     1321 ttaagttggg taagccgctc gttagcatag ttattaagcg gcgcatcttc tgcttgcggt
     1381 tcaagtcgca gcgggcgacc cagtgcctga gcaacgttat tcaaaaattc gcctcgatta
     1441 tccattcttt ttctcctgcg cctgatgttt cttaaaccaa ctacggaaac tctctccgtc
     1501 agcttcagga agatcgcgtg cttccatcca gtcgctaatc gcgccaaatt tgagtggtgt
     1561 tttgccgcca ttgataaacc agcttgccgc atgagcaccg gccatcatcc cgactttcca
     1621 caatcctgga tgactattgg cataagcgaa catttttatc gcccgttgct ctgcttttgc
     1681 ggtgatccct ttttcagcca tcacccgacg atgacgcaaa atcagttttg acagcggaat
     1741 acgcaccgga cacacgttgt cacaagctgt gcataaagag caggcgtagg gtaaatcttt
     1801 aaaatcttta tagccgccaa gtagcggaga aatcaccgca ccaattggcc ctggataaat
     1861 agagccatat ccatgaccgc caatatggcg atatgccgga caagtattca tacaagcccc
     1921 gcagcgaata cagcgcagca catcccgaaa ttcagaggcc agcacctcag aacgcccgtt
     1981 atcgacaata accagatgaa actcttcagg accatcaacg tgccccgctt cgcgaggtcc
     2041 tgtcagccag gtgttgtatc ccgtcaaacg tgcaccaacg gcactgcgcg ccagcatggt
     2101 gatcaataca tctacctcgg caaacgtggg ggcaatacgc tccattccca tcactgcaat
     2161 atgcgtttta ggcagcgtgg tacacattcg cgcattacct tcattggtca ccaggcatac
     2221 cgaacctgtc tctgccaccg cgaaattaca gcccgtaata cctatttcag cactgaggaa
     2281 atcttcgcgg attttttgcc ggatgaataa ggtcatcgct tcaggcgttt ccggcccctc
     2341 atagcccaga cgttcgtgta gcactcgacg gatctgatgg cgatctttat gaattgccgg
     2401 gaccacaaca tgagagggcg gatcttgatc cagctggaga atatattcac ccaaatcggt
     2461 ttcaatcacc tgaatgccag catcctgcaa cacatgattg acaccaatct cttcggtcac
     2521 catcgattta gatttcacca ccttccgggc atttttgcgt tgggcaacct gtaaaatgta
     2581 gcgggtagcg tcttctttgg tttttgcaaa atagacgtga ccgccgtttt gcgtcacttt
     2641 ttctgagagc tggtacagat aagcgtcgag attactcaga acatgatcac gtatctgggc
     2701 ggcccgatcg cgccactcct cccagagccc caattcatcg accatttttt gccgatttgc
     2761 cccaatacgc tgctgcgcgt ttgccaccgc tttgcgcatg atcggatctt caatttgctg
     2821 acggatgcgt gtcttaaaat ctgtattact ggttttgatc gacatcttta tatcctcagc
     2881 ggctcatcaa cacttcagca atatgcatca ctttgacttt ctgcccttcc cgttgtaatc
     2941 gcccactgat gttcagcagg caactcacgt cagcaccaat taaatactca gggcggactt
     3001 ccatcaggtg cgcaaccttt tctttcacca tctcgccgga tatttcggcc attttgaccg
     3061 agaacgtgcc gccaaatccg cagcaggtat cctgttcagc aaaggtcaac agctccagtc
     3121 cacgcacatt tttcagcagc gtaagtggct cgtccttcac ccccagctta cgggccaggc
     3181 tacaagatgg gtgatacacc gctctccctt gcaaactggc acctacatcg actaccccta
     3241 atttattaac aataaaagag gtgagatcct gcatacgcgc ggcaaccttt tcggctcgtg
     3301 atgcccattc aggttcatcc gccagatacg tcgggtaact ttttacggca taggtgcaag
     3361 agccagccgg tgaaataatg ggatcgtcgt tatcctccag tgcggcgatc agatttttca
     3421 tccctggaat cgcttctttg atataaccac tattgatcgc aggctgaccg cagcatccct
     3481 gtttctccgg gaaatttacg cgacagccga gtttttccag tagcagcacg gagtctcgtg
     3541 ccattcttga tttcagggcg tcaccaatac aggtgacaaa gaaattgaca ttcacaacta
     3601 atactccatt acttcatgcc catttattcc cgatgtttcc ccccacccgg ggatcaatca
     3661 tcaataaata tggcgttctt ttttaattgg ctagcaacat accacccctt atttgtatga
     3721 caaatgatag tctttcatcc aaacttcaaa tgcaataact aattttattt ctttcggcat
     3781 cgctcacatt ttatggggaa atcgacgatc atttttggca taagaaatcc atttaaaaat
     3841 ggctacatcc cttataccta ttcagagcta cttattcagg ggttattgag atatacactg
     3901 atggtataca aaaaaattac cgacaataat cacgtatcaa gtaatagtcg ggattttatt
     3961 taattgaagc aatttagttt gcatttttat ttaaaaactg agctagagtc catcagaaaa
     4021 aaatatataa agcccaaacc accgttggaa atagaaatag ttgaaaggct cacaactccc
     4081 ggaatgcgga ttatctttgc cctgatttct ggcggtttta ttcagggtgc aagctgtctg
     4141 accctttccc gatattctcc cggagtacaa ccaaactcgc ggacaaacgc cttgtgaaaa
     4201 gatgattcac tggcatagcc tactgactca gcgatcacca caacagggag catttcccgg
     4261 gaaaacatct gggccgctat ttgtagacgc aactttgtta atacagccag cggcgtggtt
     4321 ccggaaacat cacggaaaag ctgggcaaaa cttgcccggg acatgtgggc gatgctggcc
     4381 agcgattcga cgctccaggc gtgtcctggc atttccagca tttgctgtat taccgcacca
     4441 agacgtggat gcagaagcaa actgagaatg tttttctcag tattaacctg tgcaatccac
     4501 tcacgcaccg cgagggtaaa gaatgtagcg cagatctggc tacacagtgc atccactccc
     4561 ggcattgccg atctggattc ctgttgtaaa aacgggatcg cctccctcag ccagttatat
     4621 tccacgctgt ggttaaccgg tgctaaaaac agcgtttccg gcaaagacgt taaaaaataa
     4681 cgtgccgaat gttgcagccg aagagtacca cagacaatac aggtcgattc attatcgaca
     4741 tgactcaggc gatgagctga attttgtggt agcaggacca catttcccgg gcgtaatgta
     4801 aaaatctctc ccgtcggcat ttccagcttc gccgctcctt gcgttaacgc atgccaacga
     4861 ataactgata attccccggc accatgtgga agctgccagt cacttcctaa cacgcaattc
     4921 ttatcgatcg ttccttgtgg agcgttaagc atcaacaaac ggctaagggc atccatatca
     4981 gactcctgag tggtttttga ttacaaacac caacaatctt agtaaacaac taatctaata
     5041 ccatcaaata gttaatcatg atttttgttt
//
LOCUS       DBFIFM010000259         5055 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.259, whole genome shotgun sequence.
ACCESSION   DBFIFM010000259 DBFIFM010000000
VERSION     DBFIFM010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.259"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..121
                     /locus_tag="N7C74_004353"
     CDS             <1..121
                     /locus_tag="N7C74_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434091.1"
                     /translation="FNTLNKMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(90..1826)
                     /gene="aceK"
                     /locus_tag="N7C74_004354"
     CDS             complement(90..1826)
                     /gene="aceK"
                     /locus_tag="N7C74_004354"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HGU2434092.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAEFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLHWQNKSRDIHYIIRHLTETLGPENLSKSHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(2010..3314)
                     /gene="aceA"
                     /locus_tag="N7C74_004355"
     CDS             complement(2010..3314)
                     /gene="aceA"
                     /locus_tag="N7C74_004355"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HGU2434093.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAEAIHAKYPGKLLAYNCSPSFNWQKNLDDNTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(3344..4945)
                     /gene="aceB"
                     /locus_tag="N7C74_004356"
     CDS             complement(3344..4945)
                     /gene="aceB"
                     /locus_tag="N7C74_004356"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HGU2434094.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGSVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
BASE COUNT         1172 a         1369 c         1294 g         1220 t
ORIGIN      
        1 ctttaataca ttaaataaga tggctgaaag ctttggcttt acggataacc ctcgatacat
       61 tgcggagaaa aattatatgg aagctttact caaaaaagca tctccccata ccgtacgcta
      121 aatctttgcc tgcgccgata cgcataaaca tcttccacat gcccttcacg tatgcggttt
      181 tgtagtgcgc gccagtaatc agcgcggaac aggtcggcgt gcatctcttc aaacagcgga
      241 ccaatacgcg ggtcggcgca tagccaatgg cgaaactctt ccgggaaaac atcgcccggc
      301 gagacgctgt accacggttc gctggcaagt tcgtcttccg ggtagcgcgg cggcgggata
      361 tcgcggaaat tcacttccgt catgtagcaa atttcatcgt aatcataaaa aaccacacgc
      421 ccgtgacggg tgacaccgaa gtttttaaac agcatgtcgc ccgggaaaat gttagcagcg
      481 gcaagctggc gaatggcatt gccgtactct tcaatggcgt cgcgcagctg ctgaccttcc
      541 acttgttcca gccagatatt gagcggcacc atccgccgct caatataaag atggcgaatc
      601 acaatttgtt cgccgagatc ggtgattttt tccgctgctt cctggagcag taattccatt
      661 aatgccgggg aaatatgccg cttctccagc acaaagtttt caaactcctg ggtatccgcc
      721 attcggccca cgcgatcgtg ctctttcacc agttgatagc aggcacgaac gtgagcggca
      781 gacatctctt tctgcggcgc gaacttgtct ttgatgactt taaatacacg gtcaaagccc
      841 ggcagcgtga acaccaacat caccatgcca cgaatacccg gcgcttcaat gaactgctca
      901 ttacagccct gtagataaac gagatattcg cggtagcttt cggttttggc gtgcttctgg
      961 cagccgatag ccatatacaa ttcagcggtg gttttacctg gcagaatttc ccgtaaccac
     1021 tcgaccagcg ctgcgggcag cggcgcataa accataaaat aagaacgcgc aaagccaaaa
     1081 acaatgctcg cttcggcggt cgtcgtcaga caggtatcaa taaacagttc gccgtcatcc
     1141 gtctggtgga tcggcaataa aaatggcaat gtgccggaag gcgttattag tttgcccacc
     1201 agccaggcgg ctttattacg gtaaaacagt tcgttcgcca cctgtaaatg acttttggag
     1261 aggttttctg gccccaacgt ttccgtcaga tggcgaataa tgtaatggat gtcacggctt
     1321 ttattctgcc agtgcagacg caacggcagg tcgctgataa cgcgcatcag cagagattcc
     1381 cagccgtgat cggggtgaaa gtctttcgcc agcgggcggg gaatggtacg aaagcggcgc
     1441 tctggctggg agctaaagat aaaaagccgc tcgggagtaa gcgagcggtg gtcaaataac
     1501 cgacagtaca cggagttaaa aaagctctcc gcaatctcga agcgcgggta atccggcaac
     1561 agccgggtgt aatgctcttt gacgcgcagt aaaaattccg cgtccgtgct ttggccgttg
     1621 gtaatgcagc gcagttgctc cacgaccaga ccaacgtgat gatcgtaaag atggatacgg
     1681 tttttcatcg cctgctggac agcatgccag tcggcttgtt cgaaacgctg ctgcgccccg
     1741 gaagtcactt cgaggaatcg accatactga gcgtcgaagc cttgcaaaat ggtttgagca
     1801 atcagtaatt ccaggccacg cggcatttag cgccctcatc aggagcagag aattgccgga
     1861 tgcggcgtaa acgccttatc cagcctacgt tcggcaacgg ttgtaggcat gataagacgc
     1921 gcaagcgtcg catcaggcat cgtgcaccga ttgccggatg cggcgtatat gccttatccg
     1981 gcctacagtc agcaacgttt gtaggttgct tagaactgcg attcttcggt ggagccagtc
     2041 agcgcggtaa ccgacgacgt gccgccctga ataatggtcg tcactttatc gaagtaacct
     2101 gtacccactt cctgctggtg agatacgaag gtgtagccat ctttcgcggc ggcgaattcc
     2161 ggctgctgca ctttctcaac atagtgcttc ataccctcgc cctgggcata ggcgtttgcc
     2221 agatcaaaca tgttgaacca catgctgtgg atacctgcca gggtgatgaa ctggaacttg
     2281 tagcccatat ccgacagctg ctgctggaag ctggcaatag tgttgtcgtc gaggtttttc
     2341 tgccagttga acgacggcga gcagttataa gccagcagtt tgcccggata tttcgcgtgg
     2401 atagcttccg caaagcgacg cgccagttcc agatccggcg tggaggtttc acaccatacc
     2461 aggtcagcat atggcgcata cgccaggcca cggctgatcg cttgctcaat gcccgcatga
     2521 gtacggaaga agccttcact ggtacgctcg ccggtaataa attcgctgtc atacgggtcg
     2581 caatcggagg tgatcagatc cgccgcatca gcatcggtac gtgcaaccag cagggttgga
     2641 acgcccgtca cgtcagctgc cagacgcgcc gcgaccagtt tctgaatagc ttcctgagtt
     2701 ggcaccaaaa ctttgccgcc catgtgaccg catttcttca ctgacgccag ctgatcttcg
     2761 aagtgaactg ccgctgcacc ggcttcaatc atcgctttca tcagttcaaa ggcattcagg
     2821 acaccgccaa aaccggcttc cgcgtcggca acgatcggca ggaagtaatc gacatagcgc
     2881 ggatcgcccg gctcaatgcc cgcggaccat tggatctgat ccgcacggcg gaaagtgttg
     2941 ttgatccgct ccaccacagc tggcaccgag tttgccggat agagcgactg atccggatac
     3001 atgctggccg ccaggttagc gtccgccgct acctgccatc ccgacagata gaccgcttca
     3061 atacccgctt tcgcctgttg cagcgcctga ccgccagtca gtgcaccgag gctgttgatg
     3121 tagccttttt tcgactcgcc atgcagcaga cgccacattt tcgccgcgcc cagttgcgcc
     3181 agcgtgcatt caggattgac tgaaccgcgt aatttcacca catcttccgc gctgtatggg
     3241 cgagtaattc cttcccaacg cggttgagtc cactctttct gtaattcttc aatttgttgt
     3301 gtacgggttt tcatgtgcag atgctccata gttatgtggt ggtttacgct aacaggcggt
     3361 agcctggcag ggtcaggaaa tcaattaact catcggaagt ggtgatctgt tccatcaagc
     3421 gtgcggcatc gtcaaaacgc ccctgggaga aacgttcttc gcccagttcg ctggcaatga
     3481 ctttcatctc ttcgcccagc atctggcgga acaaggcttt agttaccggt ttgccattgc
     3541 tcaacgtttt ttgatgatgg atccactgcc agattgaggt acgggaaatt tcagccgtcg
     3601 ccgcatcttc catcaggcca taaatcggca cgcagccgtt gccggagatc catgcttcga
     3661 tgtactgcac agccacgcga atgttggcgc gcataccttc ttcggtgcgt tcaccatcac
     3721 aaggtgccag cagctgatcg gcagtaatcg gcgcgtcttg ttcgcgcatc acttcaagct
     3781 gatttttacg ggagccgaga atgtcgttga ataccgccat tgccgtgtcc gcaaggcctg
     3841 ggtgagcgat ccatgtgcca tcgtgaccgt tattggcttc cagcgattta tccgctttta
     3901 ctttgttgag cacctggtta ttgtgctctt catctttgct cggaataaac gccgccatgc
     3961 cgcccatcgc aaaagcaccg cgtttatggc aggttttaat caacaggcgt gagtaagcat
     4021 tcaggaatgg tttatccatc gttactgcct gtctgtctgg caggacgcga tcgggatagt
     4081 ttttcaacgt tttgatatag ctgaagatgt aatcccaacg accgcagttc agaccaacaa
     4141 tatggtcacg cagcgcgtga aggatttcat ccatctggaa cacggcgggc agcgtttcaa
     4201 tcagcaacgt cgccttgatg gtgccgcgcg gcagattaaa gcgatcttct gcatagctga
     4261 agacttcgct ccaccaggcc gcttcctgcc aggactgggt tttcggcaga tagaaatagg
     4321 gaccactgcc ctttgccaac agtgcctgat agttgtggaa gaaatagagc gcaaaatcaa
     4381 acaggctgcc ggggattgcc tcaccacgcc aggtgacatg tttttccggc aagtgcagac
     4441 cgcgtacccg acaaatcaaa accgctggat tgggcttgag ctggtaaatt ttgcctgctt
     4501 cattggtgta actgatggtg ccgttaaccg catcacgcag gttaatttgc ccgtcgatca
     4561 ctttgttcca gtctggtgcc agtgaatctt cgaaatcggc cataaagact ttcacattgg
     4621 cgttgagcgc gttgatcacc atcttgcgct ctacggatcc ggtaatctct acgcggcgat
     4681 cttctaagtc cgcaggaatc ccgcgaattt tccagtcagc atcacgaatg gaagctgttt
     4741 ccgaaataaa atcaggcaac gttccgttat caatatcttg ctgctgctga atgcgcgctg
     4801 ccagaagttt attgcgttgt ggcgtaaaat gcgtcaccag ctcagtcaga aattctaccg
     4861 cttcggcagt aagaatttgc ttctcctgct cgccatacgg ccttatgaaa gccagttcat
     4921 cggttgttgt tgcctgttca gtcatcgtgc agctcctcgt catggatccg aaaacttccc
     4981 cactgtgaac gaaggatcgt tgtgcgcttt tcgttcagca caactaagac tactcattta
     5041 aaaatcaaaa tcaaa
//
LOCUS       DBFIFM010000260         4960 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.260, whole genome shotgun sequence.
ACCESSION   DBFIFM010000260 DBFIFM010000000
VERSION     DBFIFM010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.260"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(90..242)
                     /locus_tag="N7C74_004357"
     CDS             complement(90..242)
                     /locus_tag="N7C74_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU2434095.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(506..1240)
                     /gene="cysH"
                     /locus_tag="N7C74_004358"
     CDS             complement(506..1240)
                     /gene="cysH"
                     /locus_tag="N7C74_004358"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HGU2434096.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKNGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1314..3026)
                     /gene="cysI"
                     /locus_tag="N7C74_004359"
     CDS             complement(1314..3026)
                     /gene="cysI"
                     /locus_tag="N7C74_004359"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HGU2434097.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3026..4825)
                     /gene="cysJ"
                     /locus_tag="N7C74_004360"
     CDS             complement(3026..4825)
                     /gene="cysJ"
                     /locus_tag="N7C74_004360"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HGU2434098.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLTAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGVVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQEEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
BASE COUNT         1062 a         1408 c         1280 g         1210 t
ORIGIN      
        1 tattcacata aaaaatataa atttaaaaaa gggtacagga gaccaaacca tcacaattca
       61 atacgcgggg ttttaccccc gcgtggtggc tacttcttcg attcgtaagc gagaactgcc
      121 ttaaactcca taccacgctc tctgatactc aactcacata acgagtcgcc taccatgagc
      181 gtgaatccca gtactgtaca acacagtact atgattgcca caagggcata ttttgttagc
      241 atgtcttgcc tccctgcaaa gaagagacta acatccgaat tgctacgttc gaacgcgagc
      301 ctcaggttga tagaaatatc gcctggggct tttgtccatc tggaacctcg cgaatgctta
      361 acgccagaca gcctcaagca cccgacgcca ttctatacct gataattctt ccagcggcgt
      421 tcacgtttaa aatcaggaat gctgctcgca aaataaaaac gcccccatca ttactgacag
      481 aggcgtttaa tttatccggc aatatttacc cttcgtgtaa cccacattcc ctttttaagc
      541 caaagaaacg cgtttcttct tccgccatgc cgggttccca tttacgggtt gtatgggtat
      601 cgcccaccga taaatatcct tcgtcccata atgggtgata tttcaggcca ttcttttgca
      661 gatactgata tatcgttcgg ttatcccagt cgataatcgg cagcacttta aatacgccac
      721 gctgaattgc cagcaccggt aaattggcgc gactgccgga ttgttcacgg cgcaggccag
      781 caaaccaggt ttgcgcgtta agttctttca gcgcccggtt catcggttcg actttgttga
      841 tgtcattgta cttttcaatg ccttcaacgc cctgttccca cagttttccg tagcgtgctt
      901 cctgccaggc tgcgctttcg gtagcacggt acactttcag gttgagcttg agtttgtccg
      961 ttaactcgtc aataaagcgg taggtttccg ggaacaagta acccgtatcg gtgaggatca
     1021 ccggaatatc cgggcgaatt tgattcacca gatgcaggct caccgccgcc tgaatgccaa
     1081 agctggaaga aagtacatat tcaccgggca gattatccag cgcccaggct acgcggcctt
     1141 cagcgtccag tttttccagt tgggcgttag tttccgccag cgccagaatg cgatctacct
     1201 tcggcagttc gttcagggcg tttagatcga gtttggacat agattcctca ctgtttgcct
     1261 tgcctgatgc gacgctcacg cgtcttatca ggcctacaag accgggctga tggttaatcc
     1321 cacaaatcgc gcgccggatc gagcaccggg cgaatgatgc ccgcacgcac cgtaaagtcg
     1381 ccgaagcctt cacccgcttc gcgctctttc gcccagcgcc ctatcagttc atcaagcgac
     1441 gccaggattt ccggctcggt gatgttttct ttatacatcc gtgggatacg tgtcccaatg
     1501 cggttgccgc caagatgcag gttgtagcga cccggcgctt tacccaccag gcccacttcc
     1561 gccagcatcg cgcgaccaca accgttcggg cagcctgtta cacgcatcac gatatgctca
     1621 tcgctgacac catgtttcgc cattaaatta tcgatgttgt cgataaaaga cggcaggaaa
     1681 cgctccgctt ccgccatcgc cagcgggcaa gtcgggaacg agacgcaggc cattgagttt
     1741 tcacgctgcg gcgtgacggc attcattaac ccgctctctt tggcgatctt ctcgatcttc
     1801 gctttctcgc tttccggcac accggcgatg atcagattct ggttcgccgt aatgcggaaa
     1861 tcacctttgt ggatcttcgc gatctccagc aggccggttt tcagcggacg ccccggataa
     1921 tcaaggatgc gaccattttc gataaacagc gtcaggtgcc agttatcatc aatgccctta
     1981 acccagccaa tacgatctcc gcgtccggtg aactcatacg gacggatcgg ttcaaatttg
     2041 atccccgcgc gacgctccac ttccgcttta aacgtctcaa cccccacgcg ctccagcgtg
     2101 tatttggttt tggcattttt acgatcggtt cggttacccc agtcacgctg agttgtcacg
     2161 acggcttccg ccactgccag cgtatgctcc agcggcagat agccaaactc actcgccgtg
     2221 cgggcgtagg ttttcttgtt gccgtgttcg atagaaagtc caccgcccac caacaggtta
     2281 aagcccacca gcttgccgtt ttcggcgatt gccacgaagt tcatgtcgtt ggcgtgcaga
     2341 tcgatatcgt tctgtggcgg gatcactacc gtggttttga atttacgcgg caggtaggtc
     2401 tggccgagga tcggttcttc atcggtggtg gcgacttttt cctgatcgag ccagatctcc
     2461 gcatacgcgc gggtacgagg cagcagatgt tcagagattt tcttcgccca ctcgtacgct
     2521 tctgcgtgca gctgcgactc gtaagggttc gaggtgcaga gtacgttacg gttcatgtcg
     2581 ttagcggtcg ccagcgcatc aagaccgacc gagtgcagca tctggtgcac cggtttgacg
     2641 ttctttttca gaatgccgtg gaactggaat gtctggcggt tggtcaggcg aatgctgcca
     2701 tagatggtgt tttcaccggc aaatttatcg atcgcctgcc actgtttggt ggtgataact
     2761 ccacccggca gacggcagcg caacagcatc gcatggcgcg gctccagctt ctgttcagca
     2821 cgttcggcgc ggatgtcgcg gtcatcctgc tgatacatgc cgtggaagcg gatcagcagg
     2881 aagttatcgc ccttaaagcc gccggtcagg ccgtcgttta aatcttccgc aatggtgccg
     2941 cgcaggtagt tgctttcaag cttcatgcgc tcggcgtctg tcagttttcc ttcgaccact
     3001 aaaggccctg gatgtttttc gctcattagt agacatctcg ctgataacgg cgctctacgc
     3061 gcagctcact taaaaattca tccgccgctt cggtgtccat gccaccaaat tcggcaatca
     3121 cttccagaag tgcctgctca acgtctttcg ccatgcgatt agcgtcgccg cagacataaa
     3181 tgtgggcacc gtcattgatc cagcgccaca gctccgcgcc ctgttcgcgc agtttgtctt
     3241 gtacgtaaac tttttctttt tgatcgcgcg accaggcaag atcgatacgc gtcagcacgc
     3301 cctctttgac gtaacgctgc cactccacct ggtagaggaa atcttccgta aagtgcgggt
     3361 tgccaaagaa cagccagttt ttacccggcg cttcgtcagc ggcgcgctgc tgcataaagg
     3421 cgcggaacgg cgcgatgccg gtgcctgggc caatcataat caccggggtt tccgggttag
     3481 cgggcaggcg gaagttatcg ttatgttcga taaatacgcg aacttcgccc tcttcttcca
     3541 cgcggtccgc gaggaagctg gaggcaccac cggcacgggc gcggccttcc acgtcgtaac
     3601 gcaccacacc aacggtgacg tgtacttcgt tctcgacttc ctcctgcgag gaggcgatgg
     3661 aatacagacg cggcgtcagc gggcgcagca gattaatcag cgcttcggcg tccagttgcg
     3721 ccggagagaa gcgcaccatg tcgacaatcg gcgtagtcgc ggcgtaatgc tgtaacttcg
     3781 ctttatcgcc caccagcggc aacagcgttt cgctgcgggt aagcgtggca tagttctcaa
     3841 caatattggc ggtgttgacg gtcagttcga agtgccactg tagcgcttcg ttcagaggca
     3901 acgttttgcc ctcgacggtg acaggttcat cgcctttcag ccacagcagt tcgacaagtt
     3961 ctttcaccag tgccggatcg ttctgatacc agacacccag cgcgtcaccc ggctggtaac
     4021 gcaggcccga gtcacctaag tcaatttcga tatggcgaac gtctttttca gagttacgcc
     4081 cggtaatttt ctggttaacc gaaaggctcg ctaccagcgg cgcgtctttg ctgtacgggc
     4141 tggtgtggat ttcatttacc acgccagtag cgacggattg cgaaggtgcc gcgacaggcg
     4201 cacgcgattt aagcgcatca accacgcggg cgcgccactc gctggcagca gcctggtatt
     4261 caacgtcggc gtcgacacgg tcgagcaggc gttcaccgcc cagttccgcc agcttgctgt
     4321 cgaaatcttt cccggactgg cagaaaaatt cataagaact atcgccgagg ctaaacacgg
     4381 caaaagcggt attttccagc ttcggcgctt ttttggagaa caggaactta tgcagcgcga
     4441 cggcttcttc cggcggttcc ccttctcctt gcgttgacgt caccacgata agcagttttt
     4501 cgctggcgat ttgtttgaat ttatagtcgc ccgcgttcac cagcttaacg ttcagttttg
     4561 ccgtcaataa atcatcgcgt aatgcttcag caacccggcg cgcattgccg gtttgcgaag
     4621 cagagataat agttataccc ggcatttctg cggctggcgc tggcgtcgct gcaagcgcag
     4681 caggctgctg attgagcacg ccccagaaat agccagaaac ccaggcaagc tgggtgggag
     4741 ttaaatcggt cgtggccgcc tgaaggcgtg ccagttgctc cgggttcaac ggaagcaacg
     4801 cggaaggtgg gacctgtgtc gtcatgcgtc gttatgttcc agtaagcaaa gctgtttctg
     4861 cgccctgtca gcgcccataa aacagaagag aaggtaaggt taacggggtg aacggtgtgg
     4921 attaaagacg ggatagcgat aactaataac caaatcgact
//
LOCUS       DBFIFM010000261         4941 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.261, whole genome shotgun sequence.
ACCESSION   DBFIFM010000261 DBFIFM010000000
VERSION     DBFIFM010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.261"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..165)
                     /locus_tag="N7C74_004361"
     CDS             complement(<1..165)
                     /locus_tag="N7C74_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434099.1"
                     /translation="MKTWGCGGLELWKNGTGFSEIANILGSKPGTIFTMLRDTGGIKP
                     HERKRAVAHLT"
     gene            complement(107..301)
                     /locus_tag="N7C74_004362"
     CDS             complement(107..301)
                     /locus_tag="N7C74_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434100.1"
                     /translation="MRALCAVNGSHPENLRFWLKACPVVLRERFHQQCKCRFNWSTQQ
                     LCENMGLRRFGTMEERNRLQ"
     gene            complement(322..603)
                     /locus_tag="N7C74_004363"
     CDS             complement(322..603)
                     /locus_tag="N7C74_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434101.1"
                     /translation="MRCGLLMAICLCLVSFSGYAATGKSDAQIKKEIIKESIESYPGN
                     CACPYNHARNGSRCGGRSAYSRNGGYDVVCYEGDVTDEMIRQWKQENAE"
     gene            complement(1142..1228)
                     /locus_tag="N7C74_004364"
     tRNA            complement(1142..1228)
                     /locus_tag="N7C74_004364"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1192..1194),aa:Leu,seq:taa)
     gene            complement(1241..1314)
                     /locus_tag="N7C74_004365"
     tRNA            complement(1241..1314)
                     /locus_tag="N7C74_004365"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1280..1282),aa:Cys,seq:gca)
     gene            complement(1368..1443)
                     /locus_tag="N7C74_004366"
     tRNA            complement(1368..1443)
                     /locus_tag="N7C74_004366"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1408..1410),aa:Gly,seq:gcc)
     gene            complement(1595..2143)
                     /gene="pgsA"
                     /locus_tag="N7C74_004367"
     CDS             complement(1595..2143)
                     /gene="pgsA"
                     /locus_tag="N7C74_004367"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HGU2434102.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(2200..4032)
                     /gene="uvrC"
                     /locus_tag="N7C74_004368"
     CDS             complement(2200..4032)
                     /gene="uvrC"
                     /locus_tag="N7C74_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HGU2434103.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(4029..4685)
                     /gene="uvrY"
                     /locus_tag="N7C74_004369"
     CDS             complement(4029..4685)
                     /gene="uvrY"
                     /locus_tag="N7C74_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HGU2434104.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
BASE COUNT         1298 a         1294 c         1101 g         1248 t
ORIGIN      
        1 tgtcaggtga gctacagccc gcttacgctc atggggtttt atgccgccag tatcccttaa
       61 catagtgaag atcgttccgg gttttgaacc caggatattc gctatttcac tgaagcctgt
      121 tccgttcttc catagttcca aacctccgca accccatgtt ttcacataac tgttgcgttg
      181 accaattgaa tctacactta cattgttgat gaaatcgctc acgaaggacc accggacagg
      241 ctttcagcca gaagcgcaga ttttcgggat gtgaaccgtt aacggcacat aatgctcgca
      301 caacaaacct gttaaaagag attactcagc attctcctgc ttccattgcc ggatcatttc
      361 atcggtaaca tcaccctcat aacataccac gtcataccct ccattacggc tatatgcact
      421 gcgtccacca catctactac cattccttgc atgattataa ggacacgcac aattgcctgg
      481 ataagattca atggattctt taattatttc ttttttgatc tgagcatcag actttcctgt
      541 tgctgcatat ccactaaaag atactaaaca aaggcagatt gccattaata aaccacatcg
      601 cataccgata cactcaatta ctaaccaatc atagcccaca tcataaaatc gagatgagga
      661 gcacaatacc tttaacaaga aggtgtcata acgataatat gtcgtgcaat tcatcaaaaa
      721 aaagatctat atcaaagaga tagaactctt atcagtgctt cagacatcgg cattcgaggc
      781 agcagaaaaa cagtaaatct gacgaatttg ccttttccca agatgttgtc agattaaaaa
      841 gatagattag cgcaggtacc tgttggtgga ttgcaggagg aaaaataaag tcgagtggaa
      901 aaatcccaga aaaatttttg tttaacagag gtcctggttc actatgcgaa atacggagga
      961 gctgcagaag aattctgctt catccttaga gtttaaacag cacgtgcact accattgttt
     1021 ctgcatatcg ccacaccatc gccactctac cgccattgca caaaatacaa atacgaaaaa
     1081 accacccgaa ggtggtttca cgacactgct tattgctttg attttattct tagctttccc
     1141 atggtacccg gagcgggact tgaacccgca cagcgcgaac gccgagggat tttaaatccc
     1201 ttgtgtctac cgattccacc atccgggctc gggaagaaag tggaggcgcg ttccggagtc
     1261 gaaccggact agacggattt gcaatccgct acataaccgc tttgttaacg cgccaaattc
     1321 ttcaggcctt tcagccagac atccgctgac gccgatgtct tttaaactgg agcgggaaac
     1381 gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc tctaccaact gagctattcc
     1441 cgcattcatc aagcaatcag ttaatcactt gattttatta tcgtctggca atcagtgccg
     1501 ccgttcgatg cgttgcattc tacttacctg gcgcgatgag tcaacgatat ttttcaccac
     1561 ttttgatcgt ttgctgaaaa ttgcgccgaa acgatcactg atcaagcaaa tctgcacgcg
     1621 cagcgctcaa atattgcaac attgaccaca gagtcagtac cgcagccaca aagaaaagtg
     1681 caataccggc gtactcaacc caaatgttcg gacgccacag cagccatgcc aacgccacca
     1741 tctgggcagt ggttttcact ttcccaatcc aggagacagc cacgctactg cgtttaccca
     1801 actccgccat ccattcgcgt agcgcagaaa taataatttc acgggcgatc atcgttgccg
     1861 ccggtaaggt cacccaccag ctgtggtaat gctcggttac cagcaccatg gcgatagcca
     1921 cgagaacttt atctgctaca gggtcaagga aagcaccaaa ccgggtactc tggttccagc
     1981 ggcgtgccag aaaaccatcg aaccagtcag tcaccgccgc gacgcagaaa atgagcgcgg
     2041 cggcaaacgg cgaccaagtg acaggcagat aaaagaccaa tacaaagaat gggataagga
     2101 tgacacggaa cagtgtaagc aacgtaggga tattaaattg cataatgacg ggtaactatc
     2161 tgttgtcagt aagattaccc ctatgttgct acagagacat caatgtttca acgaccagaa
     2221 gatcttttct gccagacctt gcgaaatacc cggcactttt gcaatttcct cgacgctggc
     2281 gttacgtaaa ccttgcaaac cgcccatata tttcaacaac atttgccggc gttttggccc
     2341 gacgccttca atggtttcca gggaactggt atttttgacc ttcgcccgtt ttttacggtg
     2401 cccgccaatc gcgtgatcgt gtgattcatc gcgaatatgc tggataacat gcagcgcggg
     2461 agaatctggc ggcaaactga atccctcacc ttccggctca aagaacagcg tttccagccc
     2521 agccttacga tctgctcctt tggcaacacc aagtagcagc ggatgatttt tatcccatga
     2581 gacatccagt tcggcgaaga catttttcgc ctgcgcaagc tggcctttgc cgccgtcgat
     2641 aagtatcaca tccgggatct tactgtcgtc gatggcttta ccataacgcc gacgcagcac
     2701 ctgattcatc gccgcataat catcgcccgg cgtgatgcca gtaatgttat agcgccgata
     2761 ctccgcacgc agcgggccgt tagcatcaaa caccacacag gaagcgacgg tttgttcacc
     2821 catggtatgg ctgatgtcaa agcactccat ccgcttcact tccggcaatt tcaacacgct
     2881 ggcaagtgct gtaagccgct ggtgaacggt agattgctgc gaaagtttgc tggttaaggc
     2941 cgtcgccgca ttggtgcgcg cgagtttcag gtaacgcgcc ctatcgccac gaggtttggt
     3001 ttgaacatta atcttgcgtc ccgccagttc tgaaagggaa tcggcgagca gcgttttatc
     3061 gctaagatta aaatcgagca ggatctcacc cggtaaggtg cgcatctggc tgccttgtaa
     3121 atagaactgg cctacgaagg tttctaccac ctcgctcagt tccgtaccgc caggcacttt
     3181 cgggaaatag ctgcggctgc cgagcacttt gccctgacga atgaacaata cgtggacaca
     3241 agccataccc gcatcgaacg ccacaccaat aacgtcgagg tcgtcgccgg tattggaaac
     3301 gaattgtttt tcggtgacgc gtcgcaccgc ctgaatttgg tcgcgaatac gtgccgcttc
     3361 ttcaaactcc agattctgac tggcagtttc catacggcta atgagttgcg taagcacctg
     3421 atcatctttg ccagacaaaa acaggcgcac atactcgacc tgctgagcgt attcttcttc
     3481 actcaccagt ccttcaacgc acggtcccag acagcgccct atctgatatt gcagacacgg
     3541 acgcgagcga ttgcgataaa cactattttc gcactggcga atggggaaaa tcttttgcag
     3601 tagcgccagt gtttcacgta cggcatagcc attcgggaac gggccgaaat attcaccttt
     3661 ggcatgcttt gcaccacgat gcatcgccag acgcgggtgg gtatcgccac tcaggaagat
     3721 aaaaggatat gatttatcat cgcgtagcaa aacgttgtaa cgcggctgat agagtttgat
     3781 gtagttgtgt tccagcaaca gcgcttcggt ttctgtatga gtaaccgtta catcaatttg
     3841 ctggatctgg gcgaccagcg cttcggtttt gcgcgaagcg aggttgctac ggaaatagct
     3901 ggaaagccgt tttttcaggt ctttcgcttt gccaacatag ataaccgtac caccagcatc
     3961 gtacatgcga taaacgccag gctggctggt tacggttttt aaaaacgctt ttgcgtcaaa
     4021 ctgatcactc actgacttga taatgtctcc gcattacaca gaccatggcg aattgccagg
     4081 tgagtcagct caacatcgcc atgaatgttt agtttactga acatacgata gcggtagctg
     4141 ttcaccgttt tcggactgag attgagctgt tctgagatct cattgacctt ctggcccttc
     4201 gtgatcatca gcataatctg caattcacgt tcagacaaac tggcaaatgg gctttctgtt
     4261 ttttctggtt cgatctggct taatgccatt tgttgagcga tgtcagaagc aatgtaacgc
     4321 tgcccagaat agacagaacg gatcgcactc acgacttcct gcggagccgc gcctttgctg
     4381 aggtagcccg cagcaccggc ctgcatgact ttcgctggta aagggttttc tgtatggacg
     4441 gtaagcatga tgatttttac atcagctgtg gaacgcgcga ttttacgcgt cgcctcaaga
     4501 ccgccaatgc ccggcatact catgtccatt agcaccacgt caacggcatt tgcccggcac
     4561 cacttaacgg cgtcttcacc gcacgatgcc tcaccgacga cttttatacc ctttatatct
     4621 tccagaatgc gtcgtatccc tgcgcgcacc agttcgtggt catcaacaag tagaacgttg
     4681 atcaaaggaa tatctccaga aatagggata acgctactga tagttagtca ttcgtatatt
     4741 agcggttttt attgcaactt tgaaacgtta aaaatggtgg gtattcgatt tttctctcgt
     4801 ttttgtcaat tcgattgtcc acagttatgg tcacgcccgt caccaacggc ttaccggatc
     4861 gtttcacagt aagccctgcg acgaaatgtg acaaaaatta cctttattca gcaaaaatga
     4921 aaatcagccc gctgaatatg t
//
LOCUS       DBFIFM010000262         4934 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.262, whole genome shotgun sequence.
ACCESSION   DBFIFM010000262 DBFIFM010000000
VERSION     DBFIFM010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.262"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            6..290
                     /gene="gatB"
                     /locus_tag="N7C74_004370"
     CDS             6..290
                     /gene="gatB"
                     /locus_tag="N7C74_004370"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HGU2434105.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            294..1649
                     /locus_tag="N7C74_004371"
     CDS             294..1649
                     /locus_tag="N7C74_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HGU2434106.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            1697..2737
                     /gene="gatD"
                     /locus_tag="N7C74_004372"
     CDS             1697..2737
                     /gene="gatD"
                     /locus_tag="N7C74_004372"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGU2434107.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            2843..3616
                     /locus_tag="N7C74_004373"
     CDS             2843..3616
                     /locus_tag="N7C74_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGU2434108.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSDVDKLALQKTRVKLITV"
     gene            complement(3698..4597)
                     /gene="yegS"
                     /locus_tag="N7C74_004374"
     CDS             complement(3698..4597)
                     /gene="yegS"
                     /locus_tag="N7C74_004374"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HGU2434109.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT         1210 a         1088 c         1285 g         1351 t
ORIGIN      
        1 tatttatgaa acgcaagatt attgtcgctt gcggaggcgc ggttgcgacc tctacgatgg
       61 cggcggaaga aattaaagag ttgtgtcaga gtcataatat tcctgttgaa ttaatccagt
      121 gtcgggttaa tgaaatagaa acctatatgg atggcgtgca tttgatatgc accactgcca
      181 gggtggatcg tagttttggc gatattccgt tagttcacgg catgcctttt gtttctggtg
      241 tcggtatcga agcattacaa aataaaattc tgactatctt acaggggtga cctatgtttt
      301 cagaagtcat gcgttatatt ctcgatctcg gccctacggt gatgttgccg attgtcatca
      361 ttattttttc taaaatttta ggcatgaagg caggcgattg ctttaaagcg ggtctgcata
      421 tcgggattgg ctttgttggc attggccttg tgattggctt aatgctggat tccattggcc
      481 cggcggcgaa agcgatggcg gaaaatttcg acctgaatct gcatgtggtc gatgtcggct
      541 ggccgggctc ttcaccaatg acctgggcgt cgcaaattgc gctggtggcg attccgattg
      601 cgattctggt taacgtggcg atgttactga cccgtatgac gcgggtggta aatgttgata
      661 tctggaatat ctggcatatg accttcaccg gcgcgttgct gcatctggca accggttcat
      721 ggatgatagg gatggcaggt gtggtaattc acgcggcgtt tgtttataag ctcggcgact
      781 ggtttgcccg cgatacccga aatttctttg agctggaagg cattgctatt ccgcacggta
      841 cgtcggcgta tatggggccg attgcggtgc tggtcgatgc tatcatcgag aaaatcccag
      901 gcgttaaccg tattaaattt agcgccgacg atattcagcg caaatttggc ccgtttggcg
      961 agcctgtcac cgtgggtttt gtgatggggc tgattatcgg catcctcgcg ggttacgatg
     1021 tcaaaggtgt attgcagctg gcggtaaaaa cggcggcggt gatgctgcta atgccacggg
     1081 tgattaaacc catcatggat ggtttaacgc ccatcgctaa gcaggcccgt agtcgtttac
     1141 aggcgaagtt cggcggtcag gagttcctga ttggcctgga tcccgcatta ctgctggggc
     1201 atacggcggt ggtatcggca agcctgattt ttattccgct caccatttta attgctgttt
     1261 gtgttcctgg taatcaggta ctgccgtttg gcgatcttgc cactatcggc ttcttcgtgg
     1321 cgatggcggt cgcggtgcat cgtggaaatc tgttccgcac cttaatctcg ggtgtcatca
     1381 ttatgagcat caccctgtgg atcgcgacgc aaactattgg tttgcacacc caactggcgg
     1441 ctaatgctgg ggcgttaaaa gccgggggta tggtggcttc aatggatcag ggcggttctc
     1501 ccattacctg gttactgatt caggttttct ccccgcaaaa tattcccggt ttcattatta
     1561 tcggcgcaat ttatcttacc ggtattttca tgacctggcg tagagcgcgt ggctttatta
     1621 aacaagagaa agtcgttctc gcagaataat ttttacctga gggggaggtt atccccctta
     1681 acaattagga gtttttatga aatcagtggt gaatgatact gatggtatcg tgcgcgttgc
     1741 agaaagcgtc attcctgaaa ttaaacatca ggatgaggtg cgggtaaaaa ttgccagctc
     1801 gggcttatgt ggttccgatt tacccaggat atttaaaaat ggtgcacatt attatccaat
     1861 aacgttaggc catgaattta gcggctatat tgatgcggta ggatccggtg ttgatgattt
     1921 acaccctggc gatgcggttg cctgtgtgcc gttattaccc tgttttactt gtccagagtg
     1981 tttgaaaggg ttttattccc agtgcgcaaa atatgatttt attggctcac ggcgtgatgg
     2041 tggatttgcc gaatatattg tcgttaagcg aaaaaatgtc tttgctctac ccacggatat
     2101 gcctattgag gatggggctt ttattgagcc gattaccgtt ggcctgcatg cttttcattt
     2161 agcgcaaggt tgtgagaata aaaacgttat tattattggt gccggaacca ttggcctgct
     2221 ggctattcag tgcgctgtcg cgctgggagc aaagagtgtg acggctatcg acattagctc
     2281 agaaaaactg gcactggcaa aatctttcgg tgcgatgcaa acatttaaca gtagcgaaat
     2341 gagcgcgccg caaatgcaga gcgttttacg cgaactgcgc tttaatcagc ttatcctcga
     2401 gacggctggc gtaccgcaaa ctgtcgaact ggcggtagag attgccggtc ctcatgccca
     2461 actggcactg gtgggcacgt tgcatcagga tctgcattta acatcggcaa cgtttggcaa
     2521 aatattgcgt aaagagctga cggttatcgg cagttggatg aactactcca gcccttggcc
     2581 ggggcaggag tgggaaacgg cgagccggtt gctgacagaa cgtaagttaa gcctggagcc
     2641 attaatcgct caccgtggaa gctttgaaag cttcgcccag gcggtgcgtg acatcgctcg
     2701 taatgctatg ccgggcaaag tgttgctcat tccctgaaac cgcgggccag cgtgatgctg
     2761 gcccggtatt gtgcaaaaca gatcattcac caatggtccc ccttcgttta cactagccac
     2821 aattgaatat gggtaaatga cgatgaattc attcgagcga aggaataaga tcatccaatt
     2881 agtgaatgaa cagggaaccg tgcttgttca ggatctggcg ggagtatttg ctgcctcgga
     2941 agcgacaatc cgtgccgatt tgcgctttct cgaacaaaaa ggcgtggtta cgcgctttca
     3001 tggcggtgcg gcgaaaataa tgtctggtaa tagtgaaacc gagacccagg aagtcgggtt
     3061 taaagagcga tttcagcttg ccagcgcgcc aaaaaacaga atagcgcagg cggcagtcaa
     3121 aatgatccac gaagggatga ccgttattct cgacagcgga agtacgacaa tgcttatcgc
     3181 cgaaggatta atgaccgcca aaaatatcac ggtgattacc aacagtctcc cggcagcgtt
     3241 tgccctttcc gaaaataaag acattactct ggtcgtctgt ggtggcacgg tccgccataa
     3301 aacgcgctcg atgcatggtt ccattgccga gcgttcattg caggatatta atgccgattt
     3361 aatgtttgtc ggtgctgatg gtattgatgc ggttaatggt attacgacct ttaatgaagg
     3421 ttattcgatt agcggggcga tggtcaccgc cgcaaataaa gtgattgccg ttctcgattc
     3481 atcgaaattt aatcgtcgcg gttttaatca ggtactgcca atcgaaaaaa ttgacattat
     3541 tattaccgat gatgccgtgt cagatgtgga taaactggca ttgcagaaga cacgggtaaa
     3601 attaattacg gtgtagtatt gttctgtatg ccggataagg tgtttacacc gcatccggca
     3661 ttacggattc tccctggttc aagagagaat agtttgatta acgcaataat ggacaatctg
     3721 gtggtaatcg acaacgcaac gccgccggaa gtatttcaat atggaagttt tgcccactca
     3781 acggttcgcc atcaagatta aaagtgattt cgtgtggggc ttgtatatca aaccacgacg
     3841 aagcgccttc gataatattc gggttatctt cgtcagattt taaggttgat acgagagccg
     3901 gaagaatttc atcgccggta aaaatgcgca gttgcagcaa gccatcgtta attaacgcgt
     3961 tcgggcacaa ttgttgaccg ccaccggcct gacgcccgtt accaatacca atgaccaggg
     4021 cgtcaccttg ccagtgaaag ttttcaccgc ggatttcaca acggtccggt tgcagagtat
     4081 ccatgcgcat taagccatga atgatgtaag agacgccacc cagcgcggct tttaattttt
     4141 ccggcgtttc tgtggtaata cgcgtcccaa atccgcctgt cgccatatta ataaaacagg
     4201 tttgtttgtt gacctgcgcc atatctatcg caatggcgtt accggcaatt gccagtttca
     4261 gcgccttatc cagtgcctca ggaatcccta cactggtggc aaaatcattg gcggttccta
     4321 atggcaaaat tcccagcgcg ggtatgtcat ccccctcaca ctgaatcaac gccgtagaaa
     4381 cttcattaat ggtgccatcg ccaccaccgg caatcaccgt tgcgacgccc aacttccggg
     4441 cctcctctac atatcgtgcg gcatcgcctt tctcccaggt gacccgcaca tggatcgtca
     4501 ttccttcctc acgcaacagc ataattgctt cgcgcaaggg tagattgtca gtacttttgc
     4561 cattaagaat cagtaagctg gcgggaaatt ctgccatgat cgtatgtgcc tttatgattg
     4621 gtctatagat agtgtagagc agaaaagaaa aggtggagtc agaacaggat gaaagtcgga
     4681 ggataaatag tcagttcaag tatggaggat tcagtggtta ccaccaggag cttgcaatgg
     4741 ggacagcgaa tactaacggc agccaccgat aaatttcaaa aaagagcata tacctaatat
     4801 tcaactaaac agtggcttct tcaatataat atattaaagc ctccatggag ttacccggaa
     4861 gggcctccat gtccgtaatt cctacttatg taggaaatgt tttacagaac atttattata
     4921 atcctattca atta
//
LOCUS       DBFIFM010000263         4928 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.263, whole genome shotgun sequence.
ACCESSION   DBFIFM010000263 DBFIFM010000000
VERSION     DBFIFM010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.263"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(359..934)
                     /locus_tag="N7C74_004375"
     CDS             complement(359..934)
                     /locus_tag="N7C74_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGU2434110.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(1003..1581)
                     /gene="terD"
                     /locus_tag="N7C74_004376"
     CDS             complement(1003..1581)
                     /gene="terD"
                     /locus_tag="N7C74_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HGU2434111.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(1630..2670)
                     /gene="terC"
                     /locus_tag="N7C74_004377"
     CDS             complement(1630..2670)
                     /gene="terC"
                     /locus_tag="N7C74_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HGU2434112.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(2693..3148)
                     /locus_tag="N7C74_004378"
     CDS             complement(2693..3148)
                     /locus_tag="N7C74_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HGU2434113.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(3171..4328)
                     /locus_tag="N7C74_004379"
     CDS             complement(3171..4328)
                     /locus_tag="N7C74_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGU2434114.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(4328..4909)
                     /locus_tag="N7C74_004380"
     CDS             complement(4328..4909)
                     /locus_tag="N7C74_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGU2434115.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
BASE COUNT         1231 a         1337 c         1188 g         1172 t
ORIGIN      
        1 ttctggacgc tggatattcg gtgaggcgta aatatggtat gacaccattt ttcataacgc
       61 tgaagtttct atacctgttg aatttgaatt ttcattgacc gggtatctta ttttccaggg
      121 cccgttcctt cataagtcgc aaaagtaaca tatatccgaa gggcatgctg ttgatatcag
      181 acacggaata ctggctttaa ccagcaacca cagataaacc cggggcctgc ataaaaggtt
      241 catgccagaa ttaacatagc tctgctttct gccccccccc tttcccatat tcacccgttg
      301 atagcggatc gatacccaaa aaaaaccccg gcattgccgg ggtcagactc agcgtctttt
      361 agacattgac gccgtgctgg gaagcaagcg ctgacagacc accggcaaaa ccctggccaa
      421 cagctttaaa cttccactca gtaccatggc gatacagttc accgaagacc attgcggttt
      481 cggttgaggc gtcttcagac agatcgaaac gggcaatttc cgtcccgttg tcgttgttgt
      541 aaacgcgcat gaagctgttg ctcaccatgc cgaagttttg tttacgcgct tctgcatcat
      601 agatggtaac ggcaaatacc agttttttga tgtctgctga gactttggtc agatcgattt
      661 tgacctgctc atcgtcgccg tcgccttcac cggtacggtt gtcgccctgg tgctctactg
      721 cgccatcagg gctggtttta ttattgaaga aaatgaaatg ggcatctgac agtactttac
      781 cgtcttcacc tactgcgaat acggaagcgt ccagatcaaa accctgacca tcggttacac
      841 gggcatccca gcccaggcca accatagcga cattcatggt tggtgcttct ttggtcagag
      901 atacgttgcc gccttttacg agagaaactg ccatttttag ctcctgcaaa cagttgaatg
      961 aggctgaatt aacagcccca gatatgaaaa gttacttttc gatcaggacg cgttaatgcc
     1021 gtactgagca catacagatg ccagaccacc agcataaccc tggcctactg cgcggaattt
     1081 ccactcacca ttgtggcgat acagctcgcc gaacagcatg gcagtctcag tggacgcatc
     1141 ttcggtcaga tcgtagcgag cgacttcagt ctggttatcg tcattaacca gacgaataaa
     1201 cgcaccggat acctgaccaa agctctggcg acgagcctga gcatcgtgga tggtcacaac
     1261 gaagatgatc ttgtcaactt cagacgggac ggcgtccagt ttaattttca gcgattcatc
     1321 atcaccatcg ccctcaccgg tgcggttatc gccggtgtgc gttacggaac cgtcggatga
     1381 cgtcaggttg ttatagaaga tgaaatctga atcgccgcgc actttgccgt ttgaggccag
     1441 caggaatgct gaagcatcca ggtcaaagtc ctgaccgtct gttgaacgcg catcccagcc
     1501 aaggcccacc aggacatttt tcattgacgg agctgcttta ctcagggaga cgttcccgcc
     1561 tttggaaaga gaaacactca taaaataacc tcttcgatta gtaattgttc aggttaacac
     1621 ttaaggggat taactcccct tttcctcaga ttcaggtttg cccgggaaca tgacgcttgc
     1681 gagaatgccc agcgccagta cacccagtac aacatacagg ctggttgttg ccgcgatgct
     1741 gtaaccatga tgccagatgt gatcggtcgc attcaggccg agttttgccg cgatgaagaa
     1801 cagcagcacg ataacggcct tctccagatg aaccaggtac tgtttcagtg cctcaaggac
     1861 aaaatacaga gtacgcagac ccaggatagc aaacatcatg gcactataga cgataagcgg
     1921 ttcacgactg acggcaatga ttgccggtac cgagtcaaac gcgaacatta cgtccgagag
     1981 ttcaaccaca gccacacaca ggaacagcgg ggtcgcatac agcgccgctt ttttaccacg
     2041 gccaatggtg acatcgctgt tttctggttt cgccagttca gcatccactt ccttctggtt
     2101 aagtaggaag gcatgccctc tgagcttcgg ccagataggg aagaagcgtt taaccatacg
     2161 gtaagccaga tgctgggagt aatcctcaat ttcatcatcg tcatcaccgc ttttaagcat
     2221 catgaccgcc gtccaggcaa cgataatagc gaagacaact tcaacgtacg gccccagact
     2281 cagcaggctc gtaccgatgg cgacaaagat gcccctgaag acgatggcac caatgatccc
     2341 ccagtagaga acacggtggc gataacgatc cggaacggcg aaccaggaga agattgccat
     2401 catgacgaac aggttatcga ccgacagcac tttctccagc gcataacccg tgacaaacag
     2461 actggcaacc tcagcaccgt ggtggatata gaggaaaccg gcaaatgcca tcgcaaccac
     2521 aacccagaat acggaccaga gcgcagcact cttcagcgag attggcttgt catcacggtg
     2581 cataaacagg tcaataaaga tggcaccgac tgaaagcgca ataaaaacaa tgaccgtttc
     2641 agtcgggaag ccgatgtgtg tggaaaccat attttaccct tagggatgtg gatcagaggc
     2701 caaattcagc cgggtcgaag cccaacgcga tagcgatctc gcgggaggcc agtttctcgt
     2761 cctgatcgaa gttaccgtca cttttcgcaa cggcaatacc aacacgtaag gccagctgag
     2821 cggcctcagg ctgatctttc agcgccagga tgtatttcat ggtttcgccc ttgccaattt
     2881 caacatcgaa atcgaagctt gaaaccagtt tattgaagaa ctcaatcacc tcattggtat
     2941 cgaagacctt cagctcttct gaagagcgca gaaagcccat cattttctgc ttttcttccg
     3001 aacttacgcc gtcactcgat acggcaatac gggcacatac agcaacggtg ccctgcatga
     3061 attttttgtt tttgaaacgg ccaacctggc gggtcagttc gtcacggcct gagttaatgg
     3121 cacctttaac tttgtcgaaa aagctcatac ctgtttcctt tgttgctgtg ttatttagaa
     3181 ccggcgctcc agcgaaatcc ccagccaaat gcccggtcca tttcgtcctg gcctttgaag
     3241 tactggttaa ttcgctcgac tttaattgcg ccgttttcgt ttacgagtct ggcaatggcg
     3301 cacaatgtgc gacggttctc accttcggtc aggcgggtct caatgggcgg ttgatccggc
     3361 acatgaatgg tgactacacc atcagtcttg tcccagctgg gaacaccttc ataaataaag
     3421 gcgtaaatca gcacttcgcg gatatgcttc cattcacgtc cattgatatg cagccactcg
     3481 ccgtctgata catcacccgt ccggtcatcg cccttgagct gaacatacgg ttcatcgcga
     3541 taatcaccga atgcattacc gagcgcctga atgaccgatt tatacccgtc ctgaagctca
     3601 acaaaggccc ccagatccag gtcgattcct ttggagccga acatacctgc aaagcctgat
     3661 ttgctactgc cccggtgcca gttcaggttg atccggattt caccgaagtt atcccgtttg
     3721 gtcaggctga ttgcaggctt ctcttttgtc agcgatacct tgctcaggct gatttttgtc
     3781 tcaactggcg gaggggttac aaccggagga gttggggctg cgggagccgg ttcgtcggca
     3841 atatttacgc cgaaatgctc tgccagcggt ttaagtccac cgttgaaccc ctgcgcaacg
     3901 aagcggaatt tccagtcatt attacgacgg taaaattccc ccagaattaa ggctgcttcc
     3961 tgacggccgc tcagttcgac actgccgcta accaggccgg tagccccttg ctccacgtcg
     4021 atggaaagat tacgaagacc ggacaccgtc tgcccaccgt cacaggtcac ggtgaacgca
     4081 attttctgga catcaggttt cagccggttg agcgcgacag taaaggttga gtactggcct
     4141 tcactgacca ggctgacggt accatcatcg ttacgtggct gaccataaaa caccatgtcc
     4201 gcatcaccct gcaccttccc gtcggcatac aggcgaaacg ccgaagcatc cacctggccg
     4261 cccgaggtga tccggacccg caactcctgg gctggaacgg gggcattgcc ccccggcgtc
     4321 aggttcatta gcgaataacc gctgaaacga tatcggagtg catatcgtca attgtgcggc
     4381 cgcgacaggc atggccaagg gcggtaaagt cccagttgcc gttattacgg cgcagcgaag
     4441 aaataacgat cccggtatgt gagccctgct cagtcagctt gtagcgagcc agctctttgc
     4501 cggtttgatc gacaacgcgg cagaaagcgt tttcgacgtc attgaacgac tgaccacgga
     4561 agctgtttac cgtaaacgcc aggtattcaa cgtttgcagg caggcgggag aggttgacct
     4621 taatcacttc atcgtcaccg tcaccctcac cggtcaggtt atcgccactg tgcacaacag
     4681 cgccgcaggt ggatttcagt ttgcggaacc agatggtgtc aattgtttta cctgtgctgt
     4741 ccattaatac acagccagca tcgagatcga ttgagtcgtt gcctccaaac aatcctccaa
     4801 gaagaccttt tttcttaacc ggatcccaac cgagaccgaa atgaagctgg cttaatgcag
     4861 atgattcttt gctgagtgat accgtctggt tcttaactaa tgaaaccatg ttttattcct
     4921 taaaatgg
//
LOCUS       DBFIFM010000264         4881 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.264, whole genome shotgun sequence.
ACCESSION   DBFIFM010000264 DBFIFM010000000
VERSION     DBFIFM010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.264"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            76..1410
                     /gene="garP"
                     /locus_tag="N7C74_004381"
     CDS             76..1410
                     /gene="garP"
                     /locus_tag="N7C74_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HGU2434116.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1426..2196
                     /gene="garL"
                     /locus_tag="N7C74_004382"
     CDS             1426..2196
                     /gene="garL"
                     /locus_tag="N7C74_004382"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HGU2434117.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKTIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2226..3116
                     /gene="garR"
                     /locus_tag="N7C74_004383"
     CDS             2226..3116
                     /gene="garR"
                     /locus_tag="N7C74_004383"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HGU2434118.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3213..4358
                     /gene="garK"
                     /locus_tag="N7C74_004384"
     CDS             3213..4358
                     /gene="garK"
                     /locus_tag="N7C74_004384"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HGU2434119.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4391..4767
                     /gene="rnpB"
                     /locus_tag="N7C74_004385"
     ncRNA           4391..4767
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="N7C74_004385"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1148 a         1176 c         1398 g         1159 t
ORIGIN      
        1 cggcataaat gaaactcata aataacatct tccccataat aataaaaatc agcataagta
       61 cccgaggtaa ataaaatgat tctggacacc gttgacgaaa aaaagaaagg cgtgcatacc
      121 cgctatttaa tattactgat tatttttatt gttaccgccg ttaactacgc cgatcgtgca
      181 acgctgtcta ttgctggtac cgaagtggca aaagagttgc agttaagtgc ggtttcgatg
      241 ggttacatct tttctgcttt tggctgggcc tacttgctga tgcaaatccc cggcggctgg
      301 ctgcttgata agtttggctc gaaaaaagtt tacacctaca gcctcttttt ctggtcgcta
      361 ttcaccttcc tgcaaggctt tgttgatatg ttcccgctgg cctgggcagg gatctccatg
      421 ttctttatgc gctttatgct cggcttctcg gaagcgccat cattcccggc gaacgcccgt
      481 attgtcgccg cctggttccc gacgaaagaa cgtggtactg cctccgccat ctttaactcg
      541 gcgcaatatt tctcgctggc gctcttttcg cctctgcttg gctggctgac tttcgcctgg
      601 ggctgggagc acgtctttac cgttatgggg gtgattggtt ttgtgctgac ggcgctgtgg
      661 atcaaattga ttcataaccc gacagatcac ccacgtatgt ctgcggaaga gctgaagttt
      721 atctctgaaa atggcgcggt ggtcgatatg gaccacaaaa agccgggcag tgcggcagca
      781 agcggaccca aactgcatta catcaagcaa ttgctctcta accgcatgat gttgggcgta
      841 tttttcggac aatattttat caacaccatc acctggttct tcctcacctg gttcccgatt
      901 tatctggtgc aggaaaaagg tatgtcgatt ctgaaagtgg gtctggtcgc ctcgattcca
      961 gcactgtgtg gttttgcggg cggcgtgctg ggaggtgtct tctcggatta tctgatcaaa
     1021 cgcggtttat ccctgaccct ggcacgtaag ctaccgattg tgctgggaat gttgctggct
     1081 tccaccatca tcttatgtaa ctacaccaac aacaccacgc tggtggtcat gctgatggcg
     1141 ctggctttct ttggtaaagg atttggtgcg ctgggctggc cggtgatttc tgacaccgcg
     1201 cctaaagaga ttgttggcct ctgcggcggc gtctttaacg tctttggcaa tgttgcctcc
     1261 attgtcactc cactggtgat tggctacctg gtaagtgaac tgcactcctt caatgcagca
     1321 ctggttttcg tgggatgttc agcgctaatg gcgatggtct gctacctctt cgtagttggc
     1381 gacattaaac gtatggaatt gcagaaataa gcaaaggtat aagcgatgaa taacgatgtt
     1441 ttcccgaata aattcaaagc cgcactggct gcgaaacagg tacaaattgg ttgctggtca
     1501 gcactctcta acccgattag cactgaagtt cttggtttgg ctgggtttga ctggctggtg
     1561 ctggatggcg aacatgcgcc aaacgatatt tcaacgttta ttccgcagtt aatggccctg
     1621 aaaggcagcg ccagcgcgcc agtagtgcga gtgccgacca acgagccggt aattattaag
     1681 cgtcttctgg atatcggttt ctataacttc ctgattcctt ttgtagaaac aaaagaggaa
     1741 gcagagcagg cggtggcatc gacgcgttat ccgccagaag gcattcgcgg cgtctccgtt
     1801 tctcaccgcg ccaatatgtt tggcaccgtg gcggattatt tcgctcagtc gaacaagaac
     1861 atcactattc tggtccagat agaaagtcag cagggcgtag ataacgtcga tgctattgcc
     1921 gctaccgaag gcgtagacgg catcttcgtc ggccccagcg atctggccgc ggcattaggc
     1981 catctcggca atgcatcaca cccggatgta caaaaaacaa ttcagcacat ttttaaccgt
     2041 gccagcgcgc acggcaaacc cagcggtatc ctcgcgccgg tcgaagccga tgcgcgtcgt
     2101 tatctggaat ggggcgcgac gtttgtggct gtcggcagcg atctcggcgt cttccgctct
     2161 gccactcaga aactggctga tacctttaaa aaataaccac caccgcaaca agagagatga
     2221 ttgatatgac tatgaaagtt ggttttattg gcctggggat tatgggtaaa ccaatgagta
     2281 aaaaccttct gaaagcaggt tactcgctgg tggttgctga ccgtaaccca gaagctattg
     2341 ctgacgtgat tgctgcaggt gcagaaacag cttctacggc taaagcgatc gctgaacagt
     2401 gcgacgtcat cataaccatg ctgccaaact cccctcatgt gaaagaggtg gcgctgggtg
     2461 agaatggcat tattgaaggc gcgaagccag gtacggtatt gatcgatatg agttctatcg
     2521 caccgctggc aagccgtgaa atcagcgaag cgctgaaagc gaaaggcatt gatatgctgg
     2581 atgctccggt gagcggcggt gaaccgaaag ccatcgacgg tacgctgtca gtgatggtag
     2641 gcggcgacaa ggctattttc gacaaatact atgatttgat gaaagcgatg gcgggttccg
     2701 tggtgcatac cggggaaatc ggtgcaggta acgtcaccaa actggcaaat caggtcattg
     2761 tggcgctgaa tattgccgcg atgtcagaag cgttaacgct ggcaactaaa gcgggcgtta
     2821 acccggacct ggtttatcag gcaattcgcg gtggactggc gggcagtacc gtgctggatg
     2881 ccaaagcgcc gatggtgatg gaccgcaact tcaagccggg cttccgtatt gatctgcata
     2941 ttaaggatct ggcgaatgcg ctggatactt ctcacggcgt cggcgcacaa ctgccgctca
     3001 cagctgcggt tatggagatg atgcaggcac tgcgtgcaga tggtttagga acggcggatc
     3061 atagcgccct ggcgtgctac tacgaaaaac tggcgaaagt cgaagttact cgttaatgac
     3121 gtcgtgcccg gatggcgtat tgcaatccgg gcctggaatc caggccgaat aagagaaacg
     3181 ccctccggcg tcatgtggta acaggcatag gtatgaaaat cgtaatcgcc ccagactctt
     3241 ataaagaaag tttatctgcc agcgaggttg cgcaggcgat agaaaaagga tttcgggaaa
     3301 tttttcctga tgcacagtac gtttctgttc ctgttgccga cggtggcgaa ggaacggtgg
     3361 aagcgatgat tgcagccacc cagggttccg aacgtcacgc ctgggttaca gggccgctgg
     3421 gcgagaaagt gaatgccagt tgggggatct ccggcgatgg caaaaccgcg tttattgaaa
     3481 tggcggcggc cagtgggctg gagctggtac ctgcggaaaa acgcgatcca ctcgtgacca
     3541 cttcacgcgg cacaggcgag ttaatcctgc aggcgctgga gagcggcgcg acaaacatta
     3601 ttatcggcat tggcggcagc gctacaaatg atggcggcgc aggcatggta caggcgctgg
     3661 gggcgaaatt atgcgacgcc aacggcaatg aaattggttt tggcggcggt agtcttaata
     3721 ctctgaatga tattgatatt tccggcctcg atccgcgctt aaaagattgc gtcattcgcg
     3781 tcgcttgtga tgtcaccaat ccgctggtgg gcgataacgg cgcatcgcgc atctttggcc
     3841 cacaaaaggg agccagtgaa gcgatgattg ttgagctgga caataacctc tctcactatg
     3901 ccgaggtcat taaaaaagcg ctgcatgttg atgtgaaaga tgtccccggc gcaggagctg
     3961 cgggtggtat gggcgcggcg ctaatggcgt ttcttggtgc ggaactgaaa agtggtattg
     4021 aaatcgtcac tacggcgctg aatctggagg aacatattca cgattgtacg ctggtgatca
     4081 ccggtgaagg gcgtattgac agccagagta ttcacggcaa ggtaccgatt ggtgtcgcaa
     4141 acgtggcgaa gaagtaccat aaaccggtga ttggcattgc gggtagcctg accgatgatg
     4201 ttggcgttgt acatcagcat ggcattgatg cggtcttcag cgtattgacc agcataggta
     4261 cgttggacga agcattccgc ggggcttatg acaatatctg ccgtgcttca cgtaatatcg
     4321 ccgcgacact ggcgattgga atgcgcaacg cggggtgaca agggcgcgca aaccctctat
     4381 actgcgcgcc gaagctgacc agacagtcgc cgcttcgtcg tcgtcctctt cgggggagac
     4441 gggcggaggg gaggaaagtc cgggctccat agggcagggt gccaggtaac gcctgggggg
     4501 gaaacccacg accagtgcaa cagagagcaa accgccgatg gcccgcgcaa gcgggatcag
     4561 gtaagggtga aagggtgcgg taagagcgca ccgcgcggct ggtaacagtc cgtggcacgg
     4621 taaactccac ccggagcaag gccaaatagg ggttcataag gtacggcccg tactgaaccc
     4681 gggtaggctg cttgagccag tgagcgattg ctggcctaga tgaatgactg tccacgacag
     4741 aacccggctt atcggtcagt ttcacctgat ttacgtaaaa acccgcttcg gcgggttttt
     4801 gcttttggag gggcagaaag atgaatgact gtccacgaca ctatacccaa aagaaagcgg
     4861 cttatcggtc agtttcacct g
//
LOCUS       DBFIFM010000265         4869 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.265, whole genome shotgun sequence.
ACCESSION   DBFIFM010000265 DBFIFM010000000
VERSION     DBFIFM010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.265"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(166..378)
                     /locus_tag="N7C74_004386"
     CDS             complement(166..378)
                     /locus_tag="N7C74_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HGU2434120.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
     gene            complement(511..1071)
                     /locus_tag="N7C74_004387"
     CDS             complement(511..1071)
                     /locus_tag="N7C74_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HGU2434121.1"
                     /translation="MAEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTQDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            complement(1174..2034)
                     /locus_tag="N7C74_004388"
     CDS             complement(1174..2034)
                     /locus_tag="N7C74_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU2434122.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDDESKVFFEKIKARHPEMDIKIPFLTVMETLQYKPAESAA
                     SVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVVA
                     VQTQWFKQYL"
     gene            complement(2093..2839)
                     /gene="traX"
                     /locus_tag="N7C74_004389"
     CDS             complement(2093..2839)
                     /gene="traX"
                     /locus_tag="N7C74_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HGU2434123.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQTWMFLAGRGAFPLFALVWGLNLSRHAHIRQSAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRTERLVLVACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPTFYGCHLAVLGILAL"
     gene            complement(2859..4247)
                     /locus_tag="N7C74_004390"
                     /pseudo
     CDS             complement(2859..4247)
                     /locus_tag="N7C74_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
     gene            4304..4555
                     /locus_tag="N7C74_004391"
                     /pseudo
     CDS             4304..4555
                     /locus_tag="N7C74_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4562..4807
                     /locus_tag="N7C74_004392"
                     /pseudo
     CDS             4562..4807
                     /locus_tag="N7C74_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         1067 a         1492 c         1142 g         1168 t
ORIGIN      
        1 tttgtgaaat taatcacaaa atataaaatc acgacttaat acggaaaaaa ctctgtgcta
       61 ctcagaactg gccggtcata ctgcccgacc ggatattaaa aaaagcaaat ctcatgcatt
      121 attcagcctt ctgaatccct ggagtaatga aggctgcttt tttcgttacc cccttcctcg
      181 gcagcgccgg tggcagtgtt cagccctttt tcgggcaaac tgctgccagt cagtccgggg
      241 tgcaacaccg gcagcccgtc gccatgcttc tgccgcttcc atccagcgtc cactgacttc
      301 cagaataacg gcccggcttc cggcacgctg gtaacgccag acctgaccgt tccgtgaact
      361 gtttctgaat ccattcatct ttatcactcc tctttaaccc gtcagtatta tggtgttaaa
      421 taatctactg aattggcttc ctttcgggta cggaagttat ttccttttaa gccttatttc
      481 cctgcctcct ctctctgagg gaggcttttt ttatttctca tcaagcacgg cctgaagttc
      541 tgcctttatc cggttctggc ggcggatttt atccagacgc gctgccgcat acgcttcctc
      601 ctcctgagaa atatgctccg tcacataccc ttccgtgtca taccggcagg caccggcttt
      661 catggcacac agatagcttt ctgaacgggt gatggccttc agcgccctgc gcagcttttt
      721 atgcgagagc gggatattcc gctgcgccac gtcttccagt aacacgtccc ggataccgca
      781 ggccagaagc cggggcgtgt caccgtcaaa taatcccggc caccagggtt tcagggtgtt
      841 cacggcctca tcctgcgtgg gcaggttcag ataaatggac agcgcctgtc tggcctgcgc
      901 tttttttgcc gccagctccg cttcccgggc tgctttctcg gccagcttct gctttttcac
      961 cttccatttt ggtggcgtgg tgacattgat gatggttttc cggctgcgga caggcgtctc
     1021 tccttccgtt ttccgcttca gtgtcagtac cggtcgtttt tgctctgcca tgcttactct
     1081 gaccttcgtg aaaatatgcc ctgcaggccg ttgaaacggc tcatcgtaac aggaagttaa
     1141 tttcaacgac ctttaacgta taaatactga caattaaaga tattgtttaa accattgtgt
     1201 ctgcacagct actacacgtt caaaaaacgc gccctcataa atatcataat ggcatgcatc
     1261 agcttcctcg tataattcct tagtaccaga agcaacagca tcatataatg ctcttccctg
     1321 ttccggtgga ttaacgctat cctggccggc aataacaacc aaaactgggc actggacgct
     1381 ggctgcactc tctgccggtt tatattgcaa cgtttccatg accgtaagga aaggtatttt
     1441 tatatccatt tcgggatgcc gggctttgat tttctcgaag aataccttcg attcatcatc
     1501 acttagtacc cgtgtaaccc ccacaaacat ctctttcccg gtgtttttct tcttctctgc
     1561 cattttattc agcgtggata gaaaagacgc tctctccgat tcattcatct ctccggtaac
     1621 cagaacatca ccgtcagcaa aagccagctg gctgacaata cattttacgc gttggtcctg
     1681 cgcagctgca ctgaagacat gcccccctcc cagcgatgtt ccccataacc caatacgctg
     1741 attatcaata cattcctgct tttctgccca attgataact gaaataatat cttcggtctg
     1801 cattgcagga accagacgcc ctcgttctcc atcactctca ccgaatcctc gataatcaaa
     1861 agtaatcgtt gcgaatcctg cttctgtgaa ggcattcgca aagcaaggga gcagaacatt
     1921 cctgataccg caaaacccat ggcataagat aatcaaagga tgttttatat ttccttcagg
     1981 aacacgaaat gtcagggcaa taccttcaga gagtttgtga tccgtaattt tcataaatat
     2041 cctgtgttat gttgtttatt aataagcaga atcagcgtca gtatcatacc agtcacaacg
     2101 ccagaatgcc cagcacagcc agatgacagc catagaacgt cgggaaaaaa tctccgggcc
     2161 agaaacgggg taatgatttc cctgcacagg acaccagacc aacggtcagc accgtcatca
     2221 caagaccagc tacagccgcc gcatcactgg aggcaaggtt aagtgccgga atcacggcca
     2281 gcaggcatgc aaccagcacc agacgttccg ttctgtcttc cgcccggtac agccggtggc
     2341 tgactgccag catcagcagc ccggcaatgc cgtaactggt gccggacaaa ggcccccaca
     2401 gtgccatcag gagaatggcc gctgctgtac gccacccact gcgcgtttca caccacgtca
     2461 gcacctgtgc cgccactgca aaggcaaaca ggatattccc ctcataccac ggaaagcctg
     2521 ccagataata cgcgaactgg gcaataattc cccatcccca cagccggtta atggccgact
     2581 gcctgatatg cgcatggcgg gacagattca gcccccacac cagagcaaac aacggaaagg
     2641 ctccacgccc ggccagaaac atccacgtct ggtccagatg cagaatgcgg tttgcgtggt
     2701 ccagtaccat cagcaccagt gccaccgttt tgatgatatc ccgctgtccg ggtgaccaga
     2761 ccagcaacga ctgcaaccag gtatcggtcc gggcagccag tgaatcgtta cgtgtcgtgt
     2821 tcgtgttatc cgttgtcatc agtgtttgtc ttcctgtatc agtctccgcc cagggttttc
     2881 tctttctgca ggtcacgaac catatcccgc tccatctgct gcagtcgctc ctgcagcagg
     2941 ttttcccgga caacctcacg gaccgcctcc cgctcccgtt gtggttcacg cagtacactc
     3001 tccggcagcg cggcttcccg ttcagaagtg acagcccgtt cacgctcctg cccggcaatc
     3061 tccctgacag cctgctctgt tctgccgtcc ggcaggtcag gtttgttctc tgccatttta
     3121 cggacaattt catctgcgcg gcgttccgtt tcacggcgga tggcctcttc agcctgccgc
     3181 tgtgccagta cctctgccgt gaccggctcg ccatttccgg ctcccggctg gacgctgttg
     3241 tccgggatat ccccccacac gcgaccaccg gtgatggcac cgggattcca cggacgacct
     3301 tcaccggcga ttctcaccac cacaccgctg tcaggattat cacgggcaat ccggacacca
     3361 tcctgcatat tatcagccag caggctctct ccgttacggc tgccctgcag ggccacgaac
     3421 tgcgcatccc cgctgccttt cacccgtcct tcaccactga atccccgcag cccgtttccg
     3481 tcatccgtgg tcagcgggtt cagccagata ccggcagact tgccgttacg gtcaaacgcc
     3541 ggcagtgcca catacggctg tggatattta cgtcccggag caataaaccg tgcaggactg
     3601 tctcccccgg caagccccgc ctgacgaaga acggcacgcc ctgccgccac gtcccgcagc
     3661 tcccgcgccg tactgaacag ccgctctgca ttcatgacct cccggtccgg tttcggttca
     3721 aacacatcgt gggctgttcc tttctgtacg gcattgttaa tggcatccgt ccagccctga
     3781 cggttatcgg tgtacacctg cacatgctgc ttcatacgcg acagggccac gtaggctgac
     3841 tcaaagccgg ccatcagttt ccggctgcct tccgtacctt caagcgcgat ggcaaaggtt
     3901 tcacttgccc cctgcgcacc gtgagcggtg atggcatagg ccaggtcaat atgttgctct
     3961 gcccgctcct gggcggggcg aatcacccgg gtctgctgtc cgcccgacag cgtgacactg
     4021 tcaccggaaa ccgctgtcac cgtccagacg ctgttggcca cataaccacg cccccggtca
     4081 ctcttcgtga agcgcatccg gtcaccggtc cccaccctga tggtgtccgg ggggtacagt
     4141 gtgacgcctt cagccaccgc ctcccggggc gaaatcagcc gcgtgttacc ttccgcatcc
     4201 tgcagagtta tcagcccgtc atccctgctg ataccggcaa tccggtgatt gaaccgcccc
     4261 ggaaatcctg gagactaaac tccctgagaa agaggtaaac aggatgacta aaaatactcg
     4321 tttttccccc gaggtccgtc aacgggcagt tcgtatggtt ctggaaagtc agggcgaata
     4381 tgactcacaa tgggcggcaa tttgttccat tgccccaaag attggctgta caccagagac
     4441 tctgcgtgtg tgggttcgtc agcatgagcg ggataccggg agtggtgatg gtggactcac
     4501 caccgctgaa cgtcagcgtc tgaaagagct ggaacgtgaa aatcgtgaac tgcgcgttca
     4561 gttcttccgg ctactgaacc gtcgatatga aaaagcgagc atcatactga cgtcaaacaa
     4621 agggttcgca gactggggag aaatgttcgg agagcatgtg ctggcaacag cgatcctgga
     4681 ccggctgcta catcactcaa ccacgctgaa tatcaaagga gagagttacc ggttcaatat
     4741 tccagacaga ctcgtggttc atttgagcct ccatgctcgc cctgaactcc gggcacacaa
     4801 tacgtgatgt ctcccgccca cttctggttc gggccactgg cgtaaaaatc ctgctccagc
     4861 agattttct
//
LOCUS       DBFIFM010000266         4860 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.266, whole genome shotgun sequence.
ACCESSION   DBFIFM010000266 DBFIFM010000000
VERSION     DBFIFM010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.266"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..246
                     /locus_tag="N7C74_004393"
     CDS             <1..246
                     /locus_tag="N7C74_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU2434124.1"
                     /translation="SMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNP
                     MPVPYGEMLVGSRRISQDISTRDEGGDGKVVVIGRQA"
     gene            complement(437..964)
                     /gene="sodC"
                     /locus_tag="N7C74_004394"
     CDS             complement(437..964)
                     /gene="sodC"
                     /locus_tag="N7C74_004394"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HGU2434125.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
     gene            1098..4571
                     /locus_tag="N7C74_004395"
     CDS             1098..4571
                     /locus_tag="N7C74_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2434126.1"
                     /translation="MGKGGGRAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTSDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIAQYCDQMVPDGFGGTEPRMTFNAYLSQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDSPSDVVWPYTNSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLW
                     VIKTGLLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTMTGGRVLSIDAASRTLTLD
                     REVTLPETGAATVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSLPSL
                     RRRLFRCVSVRENTDGTFAITAVQHVPEKEAIVDNGARFEPQSGTLNSVIPPAVQHLT
                     VEVSAADGQYLAQVKWDTPRVVKGVRFSLRLTSGSGEGSRLVTTAITADTEHRSSGLP
                     PGEYTLTVRAINSYGQQGEPATTTFRINAPAVPATIELTPGYFQITAVPRLAVYDPTV
                     QFEFWFSETKIADTSQVETSARYLGTGSQWTVQGSRIKPGTDFWFYVRSVNLVGKSAF
                     VEVSGQPSNDGEGYLEFFREKIGKLHLAQGLWELIDNSQLADEMAEMKTTITETRNEI
                     TQTVSKTLENQSAIIQQIQRVQKDTNDDLAALYMLKVQKTKNGIPYVAGIGAGIEDTD
                     GQPLSNILLLADRIAMINPEDGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSANPPT
                     FSLTPEGRLTARNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKA
                     FPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLIVR
                     KNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVV
                     MKKATAGISIS"
     gene            4639..>4860
                     /locus_tag="N7C74_004396"
     CDS             4639..>4860
                     /locus_tag="N7C74_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU2434127.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHVSTNVPG
                     SDELNGINVKYRYEFTDTLGMVTSFSYAGD"
BASE COUNT         1148 a         1240 c         1460 g         1012 t
ORIGIN      
        1 agcatgattc tgggcggtgt ggcccagatg ctggccccaa aggcaaaaac accggattac
       61 cgcgcaacgg ataacggcag acagaacacg tacttttcct cgctggataa catgattgcc
      121 caggggaacc cgatgccggt gccttacggt gaaatgctgg ttggctcccg gcgaatctcc
      181 caggacatca gtacccgtga tgaaggcggt gacgggaagg tggtggttat cgggcggcag
      241 gcataaaagc gaaaaaatcc cgcagtgctc acggacagga actgcgggag cgttacgaag
      301 attgagtgta aggaattatt cttatgtcac gacaaaaaca ttaactcaga gagggaggat
      361 gtgccgttct tttcagggag aaaggattta tcgtcctgag gaataaaggt aaggggcccg
      421 ccccttacct gactgattat tgaatgatgc cgcaggccat tctcgcacca ccaccgccca
      481 ggggctccgg atggtcatga tggttatcac cgccagcatg aagcatgaga gaacgccctt
      541 taatctcttt taatgagttc agtctcgggg ccaggaccgg gtagttcgct tttccgtcat
      601 gcgtcacgaa cagcgcaggg aggtcgccca ggtgtccatc cggagaccag gggccaagat
      661 gtttgccggt gtttttcggg tcaaagtgac cgccagccga taatgctgcg accggttttc
      721 cgtctttcag tgccggggcg caatttcctt tttcgtgcac atgaaaacca tgaatgcctt
      781 cagacagaga gtgaagggct ggtgtgaaca gcagaccgta gggggtctcc tgaatggtta
      841 tttttccaat gctgacttct tttccgtcag cactgacaag gttcattggg acttcctgtt
      901 ctgctgcgta tccgcatgat gctgctgtca gcatggcaat ggcagcaatg attttacatt
      961 tcataaaacc ctcattaatt ccgttaacag actgagcttg ctggttacag ggtaacaaac
     1021 agcgttctga tgatatcgcg caatagctgt gcaatatcct atcactgcga ttaataatac
     1081 caattgagag gaacattatg ggtaaaggtg gcggcagggc gcacacgccg gttgaggcaa
     1141 aggacaatct taagtccacg cagatgatga gcgtgattga tgccattggt gaagggccga
     1201 ttgaaggtcc ggtgaagggg ctgcagagta tcctggtgaa caaaaccccg ctgacggaca
     1261 cggacggtaa tcctgtgata catggtgtga cagcggtctg gcgcgccggg gagcaggagc
     1321 agacaccacc tgaaggcttt gagtcttccg gagctgaaac cgcactgggc gtggaagtga
     1381 cgaaggcaaa gccggtgacg cgcaccatta catccgcgaa cattgaccgc ctgcgggtca
     1441 ccttcggggt gcagtcactg ttggagacca cctcaaaggg cgaccgtaat ccctcttctg
     1501 tccgactgct gattcagttg cagcgtaacg gtaactgggt gacggaaaag gatgtcacca
     1561 ttaacggcaa gaccacctcg cagtttctgg cgtcggtgat tctggataat ctgcctcccc
     1621 gtccttttaa catccggatg gtccgggaga cggcggacag cacctcggac cagctgcaga
     1681 ataagacgct ctggtcgtca tacaccgaaa tcatcgatgt gaaacagtgc tacccgaaca
     1741 cggcgattgt ggggctgcag gtggatgcgg agcagtttgg tggccagcag atgacggtga
     1801 actaccatat ccgaggtcgc atcatccagg tgccgtcaaa ctatgacccg gaaaaacgca
     1861 cgtacagcgg catctgggac ggcagcctga aaccggcata cagcaacaac ccggcctggt
     1921 gcctgtggga catgctgact cacccgcgct acggcatggg aaaacgcctg ggggcggcgg
     1981 atgtggacaa gtgggcgctg tatgccattg cgcagtactg cgaccagatg gtccctgatg
     2041 gtttcggggg cacagagccg cggatgacct ttaatgcgta cctgtcacaa cagcgtaagg
     2101 cgtgggacgt tctcagtgat ttctgctcgg cgatgcgctg tatgccggta tggaacggcc
     2161 agacgctgac gttcgttcag gacagcccgt cggatgtggt gtggccgtac accaacagtg
     2221 atgtggtggt ggatgataac ggcgtggggt ttcgctacag cttcagcgcc ctgaaggacc
     2281 gccacacggc ggtggaggtg aattacaccg acccgcagaa cggctggcag acctccacgg
     2341 aactggtgga agacccggaa gccatactgc gctacggacg caacctgctg aagatggatg
     2401 cgttcggctg caccagtcgc ggtcaggccc accgtgccgg gctgtgggtg ataaagaccg
     2461 gactgctgga aacgcagacg gtggatttca cgctcgggtc acaggggctg cgtcacacac
     2521 ccggtgacat tattgaaatc tgtgataacg actatgccgg gaccatgacc ggcggacgtg
     2581 tcctgtccat cgatgccgcc agccgcaccc tgacgctgga ccgtgaggtg acactgccgg
     2641 agaccggtgc cgccacggtg aacctgatta acggcagcgg taagccggtg agtgtggaca
     2701 tcaccgcaca ccccgcgccg gaccggatac aggtcagcac cctgcctgat ggtgtggaga
     2761 catacggtgt atggggacta tccctgccgt cactgcgccg tcgcctgttc cgctgtgtct
     2821 ccgtccggga aaacacggac ggcacctttg ccatcacggc ggtgcagcac gtaccggaaa
     2881 aagaagccat cgtggataac ggtgcccgct ttgagccgca gtcaggcacc ctgaacagcg
     2941 tcatccctcc ggcagtgcag cacctgacgg tggaggtgag cgcagctgac ggccagtatc
     3001 tggcgcaggt gaaatgggac acgccgcggg tggtgaaggg cgtgcgcttc agtctgcgcc
     3061 tgaccagcgg aagcggagaa ggcagccgtc tggtgaccac cgccatcact gcggatacag
     3121 agcatcgttc cagtggtctg ccgccggggg aatacaccct gacggtcagg gcgattaaca
     3181 gttatggcca gcagggggaa ccggccacca ccacgttcag gattaatgca cctgcggtac
     3241 ccgccacgat tgagctgaca ccgggctatt ttcagataac agcggtcccg cgtcttgcgg
     3301 tgtatgaccc gacggtacag tttgagttct ggttttcgga gacaaaaatc gcagatacat
     3361 ctcaggtgga aacctctgcc cgttatctgg ggaccggcag tcagtggact gtccagggaa
     3421 gccggattaa gccggggacg gatttctggt tttacgtgcg cagcgtcaac ctggtgggga
     3481 aatctgcgtt tgtggaagtc agcgggcagc ccagcaatga tggtgaaggg tatctggaat
     3541 ttttccggga aaaaatagga aaactgcatc tggctcaggg gctatgggag ctgatagaca
     3601 acagccagct tgcggatgag atggcggaga tgaagaccac catcacggaa acccgcaatg
     3661 aaatcacaca gacggtcagt aaaacgctgg agaaccagag cgccatcata cagcagatac
     3721 agcgcgtgca gaaggacaca aatgatgacc ttgctgcact ttacatgctg aaggtacaga
     3781 aaacaaaaaa tggcataccc tatgttgccg gtattggagc ggggattgag gatactgatg
     3841 gccagcccct gagcaacata ctgctgctgg ctgaccgtat tgcgatgatt aacccggagg
     3901 acggcaacac cacgccgtta tttgtggcgc aggggaatca gttgttcatg aacgatgtgt
     3961 tcctgaaacg actgtttgcg gtgagtatca cgtcatccgc caatcccccg acgttttccc
     4021 tgacgccgga gggcaggctg accgcaagaa atgctgatat cagcggtaac gtgaatgcga
     4081 attccgggac gctcaacaac gtcacgatta acgagaactg tcgggttctg ggaaaactgt
     4141 ccgcgaacca gattgaaggc gatctcgtta aaacagtggg caaagctttc ccccgggatt
     4201 cccgtgcacc ggagcggtgg ccatcaggga ccattaccgt cagggtttat gacgatcagc
     4261 cgtttgaccg gcagattgtt attccggcgg tggcattcag cggcgctaaa catgagagag
     4321 agcatactga tatttactcc tcatgccgtc tgatagtgcg gaaaaacggt gctgaaattt
     4381 ataaccgtac cgcgctggat aatacgctga tttacagtgg cgttattgat atgcctgccg
     4441 gtcacggtca catgacgctg gagttttcgg tgtcagcatg gctggtgaat aactggtatc
     4501 ccacagcaag tatcagcgat ttgctggttg tggtgatgaa gaaagccacc gcaggcatca
     4561 gtatcagctg aattttataa cccatatacg ggcgccagaa atggcgcctt ttttattgca
     4621 gaaaagcgag aggtaattat gcgtaaactt tatgccgcca ttttgtccgc agccatttgt
     4681 ctggccgtat ccggtgcgcc tgcatgggcg tctgaacatc agtcaacgct gagcgcgggg
     4741 tatcttcatg tctcgacgaa cgttcctggc agcgatgaac tgaacgggat taacgtgaaa
     4801 taccgttatg agtttacgga cacactgggg atggtgacgt cgttcagcta tgcaggagac
//
LOCUS       DBFIFM010000267         4795 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.267, whole genome shotgun sequence.
ACCESSION   DBFIFM010000267 DBFIFM010000000
VERSION     DBFIFM010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.267"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..577)
                     /locus_tag="N7C74_004397"
     CDS             complement(<1..577)
                     /locus_tag="N7C74_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434128.1"
                     /translation="MYGLSIMKPDGSVWISPGFTPQCLINKGTIPATEKSFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRGTNPGVTTVYAFANMVTPPSEY
                     GIAMYNPDGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPY"
     gene            complement(583..861)
                     /locus_tag="N7C74_004398"
     CDS             complement(583..861)
                     /locus_tag="N7C74_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434129.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPVFFLDRFTAESGSKTYTNKPDGK
                     SLQAVCCLFPWNNVFADRKVPKITINGNTVTWSNLEQGMGSYIYTFWG"
     gene            complement(876..2144)
                     /locus_tag="N7C74_004399"
     CDS             complement(876..2144)
                     /locus_tag="N7C74_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU2434130.1"
                     /translation="MTRKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWNAEKGTQ
                     AQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQGQAVKDAAIKTTEVVGTLREETT
                     RTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VSRKVVADEVKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYS
                     NQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
     gene            complement(2141..3766)
                     /locus_tag="N7C74_004400"
     CDS             complement(2141..3766)
                     /locus_tag="N7C74_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434131.1"
                     /translation="MPYIDITTMRGMMPRVVTSMLPEHSAVLAEDCHFRFGVITPERQ
                     ISGVEKTFTIKPKTIFHYRDDFWFAWPDVVDVIRSPIAQDPHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLAPVPLQNANIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPTKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDVNGNTALATEKIISPEQWQSQFNPMSIVAYSWRGD
                     YIGCYTKPDGKQDVFVFSPANMDIRYLSTPFDCAWIDLAKDMMRVVTGDKMSVLAGDS
                     LPSMIRWHSKIFSLPERTSFSCIRVKSPAPERVGITVMADDVPVIHFAPGTFKGSVVR
                     LPAATGQNWQVMVSGFGQVERITLSTSMSEMPV"
     gene            complement(4061..4249)
                     /locus_tag="N7C74_004401"
     CDS             complement(4061..4249)
                     /locus_tag="N7C74_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434132.1"
                     /translation="MPENRRIILDSKESWLIICDSQLGHLMRSMYQGRRFIQLNLEKL
                     KGVHDVALPVKWEFTRRQ"
     gene            complement(4388..4657)
                     /locus_tag="N7C74_004402"
     CDS             complement(4388..4657)
                     /locus_tag="N7C74_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU2434133.1"
                     /translation="MNILRKLMQSLCGCGKHDDCENGQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            complement(4659..>4795)
                     /locus_tag="N7C74_004403"
     CDS             complement(4659..>4795)
                     /locus_tag="N7C74_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU2434134.1"
                     /translation="FLDPKDATRVQGFFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE
                     "
BASE COUNT         1228 a         1347 c          966 g         1254 t
ORIGIN      
        1 tatacggctt tcgaatatcc catgttgcac cgccggaata atgctcacag gaatatattt
       61 tatttcctgt agcaccagtg gatgtggtat aaatcggtcg atcataagga gttcttttcc
      121 agttataata cccgaccagt gcaggcatga ttgcgcatgg atatccaagg tccttttcaa
      181 atttgatatc aacaggtatt aacttcgcgt caagcagcat catttcgcca tgataaatca
      241 tctcaccgtc cgggttatac atggcgatac catactcaga aggtggagta accatattcg
      301 cgaaagcata aaccgtcgta acaccagggt tcgttcccct gacaattaca tgaagcctta
      361 gtgcatgata tccatcaatc tgttcatgcg tgtacatgac atcggccttc ttctctgttc
      421 tgataaagaa aaaacaactt ttgcctgacg ggattgatgt tttaaaaaaa gacttttcag
      481 tcgccggtat ggtgcctttg ttgatcagac actgcggcgt aaaacctgga cttatccata
      541 cgctgccatc cggcttcata atgctcaaac catacatgac acttatcccc agaatgtata
      601 aatataagat cccataccct gctcaagatt cgaccacgtc accgtattgc cattaatggt
      661 tatcttcggt actttccgat ccgcaaatac attattccag ggaaataaac aacatacagc
      721 ctgcaatgat ttcccgtcgg gtttattcgt gtacgtcttt gagccagact ccgctgtaaa
      781 tctgtccagg aaaaataccg gagtcagcac gcccgtaaca ttaacgttat ttctgttata
      841 aatggcaaaa ccgtattcca atactcacct cctgatcagc gtaatctgcc tatgcgaaca
      901 gccagtcgtc cattctgatc ataaacctca attttatcat tgcggatcac cagtcctaca
      961 ttctgattag agtaacgaat tgtcagttgc ccttgtgacg taacactgaa aaggcctcca
     1021 atattcaggt taccctgaga atcaacctga aagtttccgt tctgaataac ggcactccgg
     1081 ataactggcg aagtgatact taccccggct tttacctcat ccgccacaac cttccgcgac
     1141 accagtgttt caatcaccgc gtcataaatc atcgctttcg ggatcacaac cttgccacct
     1201 gataccgcaa acggataggc ggtgttatcc gggttgttcg ggtcaaagac aaacaactgc
     1261 gacgcagaaa ttgcaacctg acttacaggc ctgccttcac tgtcttttcc ggcgacaatc
     1321 ccgatccccg cagtgatacc atcaactccc gctttttttg accacattgc cagaaacgcc
     1381 tcaccgcctt ctttatccag tttagtgatg cgcttgtcga cctcattaag cgattcactg
     1441 gttgacgaat ccagtgtgct aatgcgggtt tcaataccac caatcgttct tgtcgtttct
     1501 tccctgagag tccccacaac ttcggttgtc ttaattgcag catcctttac agcctgcccc
     1561 tgcgcgtttt ttatttcttt acgcagctcg gacacaaccg gcgactttgc agcctcatcg
     1621 cggatctggt caatgatggc cttcacgccg atctgtgttt gtgcctgagt gcctttttca
     1681 gcattccatg gacctttcac tcctgccgcg ttaacaaaac gtatccagta aaatcccgac
     1741 cagccagggt caaccggatc gccgtaaacc tgccccggcg tcgtggcaac cagcactgca
     1801 tcagcaaggt catcctccgt accccgccag atttcagtca gtgaatgtcc gcgataatta
     1861 ggcatatccc attcaagaag aaccgagcca aatcctcctg tcgccttaaa attcagcggt
     1921 tttgtgggaa aatcaacagt cattaaagta ctgtcaatct caatacccgg attcagtgca
     1981 tatgaggcac cacccgatgt tcgacgccgg gcgagtttaa gaccaaccag ttcctcacgg
     2041 gtcacaaatg cgtggcgtcc gtcaccacgc tgcccggtgc caatttccat gttctccaca
     2101 actgtggata aatccttccc cgcacgccac ggttttctgg tcataccggc atctccgaca
     2161 tcgatgtact cagggttatt cgttccacct gcccgaatcc ggataccatc acctgccagt
     2221 tttgcccggt tgctgccgga agtctcacca cacttccctt aaacgtaccc ggcgcaaaat
     2281 gaatcacagg aacatcatca gccataacag tgatccccac ccgctcaggt gccggagatt
     2341 tcactctgat acaggaaaaa gaggttcttt caggtaatga aaaaattttt gaatgccacc
     2401 ttatcatgga cggcagagag tccccggcaa gcactgacat tttgtcccct gtcaccacgc
     2461 gcatcatatc ttttgcaaga tcaatccatg cacagtcaaa cggcgtgctg agataacgga
     2521 tatccatgtt cgccggactg aatacaaaca catcctgctt accatccggt tttgtgtaac
     2581 aaccgatata gtcaccacgc caggaataag ccacaatgga catcgggtta aactgactct
     2641 gccactgttc tggtgaaata atcttttccg ttgccagtgc tgtattaccg tttacatcaa
     2701 cagataccag accgtttgtc ccggcataca gtacgaatcc ctccatcgcc accatgcttc
     2761 ttcggctcag gcatgcctgc atggaaggaa ttctggagcc agaaattgtg gaaggcgata
     2821 caccactgaa cagataaggc tccccctttg tcgccaccac cagtgacgtt cccagcggac
     2881 agatagccac aatatcttct gccgtcgtgt gacgattcac ttccggccat gcatacggca
     2941 gatacgcttc cgaaaacatc acttcattac cggcaaaccc ggcagcaata ccattagcca
     3001 tcaggcaaag acccgtcata ttctccggtg gcggcaggta atcccatgtt gccagggaag
     3061 gcccaaggtt tttcgtcggt attttgtccg tgtaactgag cactgacgca tccagttcag
     3121 ccacaagtaa aaaatccgct tctcctccac ctgatgcaga gcgataaatc cggcgacgtt
     3181 taatattggc attctgcaat ggcaccggag ccagcgtcag ttgtaccgca gtccccggag
     3241 tacggagtgt tacctccaga gacgccggac caggcggacc ttcttcacca taatctgaga
     3301 caaaggtttc agtataaaac cgggtttcgt catcattcgg gttatcgtca gaaacatcac
     3361 cgccctgctg aacagtacag acaggagctg tcgtcggcgc ggggatcccc agacgatacg
     3421 atgatgtcgg gtgattcccg tccccttttg tggcaatagt cgcatccgtc actttaggaa
     3481 aacgcccgtc agtgtagtaa atacgcccgt gggggtcctg agcgatcgga ctgcggatca
     3541 catccaccac atccggccat gcaaaccaga aatcgtcacg gtaatgaaaa attgtttttg
     3601 gcttaattgt gaatgttttc tcaaccccgg atatctgacg ttctggtgta ataacaccaa
     3661 accggaaatg gcagtcctcc gccagtacag cggaatgctc gggcagcatg gatgtcacaa
     3721 cgcgcggcat catcccacgc atcgtggtga tatcgatata gggcatagaa attcctttga
     3781 ctgcttcagg cttaaaaatc aggggattta tgatggaatt cgctaaaatc gcgatcggca
     3841 tcactgaaac aaaatgcctt gcatgacatt ctgatgatga ctaaataact tcattgatta
     3901 ttgcaatttt ctttcggttt gggggagcat attgctcccc ttttttcact gcattatcat
     3961 tgccgctgta gcaacgagaa gcatcactcc gcacacgcaa gatgtttcac actcacacca
     4021 tacagtccat ttttcgccat tcccgtatac agaaagctat tcactgtctt cgtgtgaatt
     4081 cccatttcac tggcaaggcg acatcatgta cccctttcaa tttttccaga ttcagctgaa
     4141 taaaacggcg tccctgatac atactgcgca ttaaatggcc caactgacta tcacagatta
     4201 ttaaccagga ttctttacta tccagaataa tcctgcgatt ttccggcata aaaaactctt
     4261 gaaccagaat tacacggcat ccttcactcc atcgtattaa catgtttatt acttcactct
     4321 ctaataaccc cccctataca tgacaacaaa aaccggagcc gggctccggt ttttgtgaag
     4381 ctgtcggcta tttcatcccg ccaatatttt cccacgtccc gtcagcacgc aggatttgca
     4441 gcggtcttac cacacactgt atctgctttt tatctgcatc cagtatcacc acctgtgtga
     4501 ttaccctgtc ctgctccgga ataatgccat tctcatctga ctccagaatg tctgccggtc
     4561 ccagtcgcag ttgtgctgta agcgactgcc cgttttcaca gtcatcatgc tttccgcaac
     4621 cgcacagact ctgcataagc tttctcaaaa tattcatgtc attctcctgt tctgcctgta
     4681 tcactgccca cttcatccag ccccttgaca tcctgccacg gcccgtcacc aaacctgacc
     4741 tgcaaatgct gaaaaaaacc ctgaacccgt gtggcatctt tggggtcaag aaagg
//
LOCUS       DBFIFM010000268         4785 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.268, whole genome shotgun sequence.
ACCESSION   DBFIFM010000268 DBFIFM010000000
VERSION     DBFIFM010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.268"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(158..811)
                     /gene="ribB"
                     /locus_tag="N7C74_004404"
     CDS             complement(158..811)
                     /gene="ribB"
                     /locus_tag="N7C74_004404"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HGU2434135.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(915..1063)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1185..1484
                     /gene="ubiK"
                     /locus_tag="N7C74_004405"
     CDS             1185..1484
                     /gene="ubiK"
                     /locus_tag="N7C74_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HGU2434136.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRSSELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(1519..1719)
                     /gene="glgS"
                     /locus_tag="N7C74_004406"
     CDS             complement(1519..1719)
                     /gene="glgS"
                     /locus_tag="N7C74_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HGU2434137.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            1988..2602
                     /gene="yqiJ"
                     /locus_tag="N7C74_004407"
     CDS             1988..2602
                     /gene="yqiJ"
                     /locus_tag="N7C74_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HGU2434138.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHTCIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDHFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNP"
     gene            2644..4305
                     /gene="yqiK"
                     /locus_tag="N7C74_004408"
     CDS             2644..4305
                     /gene="yqiK"
                     /locus_tag="N7C74_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HGU2434139.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLKIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGTAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSLLTPTTPVAENVE"
     gene            4544..4603
                     /locus_tag="N7C74_004409"
     CDS             4544..4603
                     /locus_tag="N7C74_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU2434140.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
BASE COUNT         1353 a         1144 c         1122 g         1166 t
ORIGIN      
        1 tgattttgat cataatgaaa aagaatgcct gatattaatg aaattttctc atagaaagag
       61 aagcggaatt aacatcttgc atagtaggga aaaataaaaa ccgaagcctg ctacaacttc
      121 ggtttcttga ttaaggcagt aaattaagca gcggttttca gctggcttta cgctcatgtg
      181 cctgacggta tgccaccagg tcttcaatag tcacgagcgc catgttgtgt ttattggcaa
      241 actcaatgca ctctggtgca cgcgccatcg tgccatcgtc attagtcagc tcacacagca
      301 caccagccgg tttgaagcct gccagcgtca ttagatcaat agttgcttca gtatgaccgc
      361 cacgcgtcag tacaccacca gcctgagcgc ggagtgggaa aacgtggccc gggcgattca
      421 gatcagaagg ttttgcgcca tcggcaatcg ctgcgcgaac ggtcgtaata cggtcagcgg
      481 cagaaacacc ggtagtcaca ccttcagccg cttcaatggt cacggtaaaa ccggtgccat
      541 aggcgctggt gttattttct accatcattg gcagatcgag ttgtttacgg cgatcttcag
      601 taatgcacag gcaaacaata ccactaccgt ggcgaatggt cagcgccatc tgctcaacag
      661 tcatggtttc tgccgggaag atcatatcac cttcgttttc acggtcttca tcatcaagca
      721 ccattacacc gcgtccttca cgcagcgcag ccagtgcatt ttcaacacgt tcgaaaggcg
      781 taccaaaaga ggaaagtagc gtctgattca tggtaaaaaa acctcactaa aattatggtt
      841 accagaatca gggcagtctt aggagtggcg gcatatagcc aaaataacgt gagcgggtcc
      901 atgcccgaca gaatcgttac tctctcccat ccggactcta accgtcggcc ccggaattac
      961 accggatctg ctgtcctttg agttcgcacc caaagcgctc gcgggctttc aactgagttg
     1021 atttaccgcc ggtggggaat ttcgccccgc cctgagaata agcggattca ctataacgct
     1081 aatgattagc ggcagcaacg catagcttca cataattctg gtttatgact tacccttatc
     1141 gcactacaat ggcactcaac acctatcagt acagggaaac tacaatgatt gacccgaaaa
     1201 aaattgagca aatcgctcgc caggttcacg aatcaatgcc taaaggaatc agggagttcg
     1261 gggaagatgt ggagaaaaaa atccgccaaa ccctacaagc gcagctgacg cgtctcgatc
     1321 tggtaagccg cgaagaattc gacgttcaga cgcaagtcct gttgcggact cgtgaaaaac
     1381 tggcgttact ggagcagcgc agcagtgaac tggaagcgcg caataattcg gtagcagatc
     1441 ttcaatctcc tccggctatc ccgcctattg ataaagcaga ataaagcaac acaaaaacgg
     1501 gccagacggc ccgtaatatc agtgttctaa ttcaagttct ctggtttgca tcatctgttg
     1561 gcaataccag gcataacgtg cgcaaaacca ggttctatgt tcttcatcca tgttaccagt
     1621 aacggccaga atatcgaccg ggcgaccttc tgcgtgcatt ctggcaaaac tacgcgctag
     1681 gaaatcgaaa ttatttaaag aattaagact atgatccata agcacatcct ccggttatcg
     1741 catacctttt aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc
     1801 tttaaacata acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa
     1861 aaccatatta aaagactttc tcttccagaa cctctctaac atatgttatt attaacaaag
     1921 acaaatgtaa gcaaggaaac tatcacaaag taaatattgc gtcactaaat ggacattgga
     1981 gtgacatatg attttattcg ccgactataa caccccttat cttttcgcta tttcctttgt
     2041 actgttgatc ggccttttag aaatttttgc gcttatctgc ggtcacatgc tctccggtgc
     2101 tcttgatgca catcttgacc attatgattc cataaccaca ggtcatatta gccaggcact
     2161 tcattatctc aatatcggaa gattgccagc tctcgtcgtt ctttgcttac tggctggttt
     2221 ttttggtctt attggcatcc tgttgcagca tacctgcatc atggtctggc aatcaccact
     2281 ctcgaacctg tttgtggttc ctgtcagttt actatttacg attattgcgg tgcattatac
     2341 cggtaaaata gttgcgccct ggattccacg cgatcatagc tcagctatta cagaagaaga
     2401 atatattggt agcatggcgt taattaccgg tcatcaagcc acttcaggta atccctgcga
     2461 aggaaaactc accgatcatt ttggccaaat acattattta ctactggagc ctgaagaagg
     2521 aaaaattttc actaaaggag acaaagtgtt aattatttgc cgactttctg caacgcgata
     2581 tttggcggaa aacaatccct agccccaaat attgtaattt attaatcaaa aggaaattta
     2641 ataatggatg atattgttaa ttccgtgccc tcctggatgt ttaccgcgat tattgccgta
     2701 tgcattctgt ttattattgg aattattttc gccaggctct atcgtcgcgc ttcggcagag
     2761 caagcatttg tccgtaccgg cttagggggg caaaaagtgg taatgagcgg tggcgcaatc
     2821 gtgatgccga tctttcatga aataatcccc atcaatatga atactctgaa gctggaagtc
     2881 agccgctcaa ccattgatag cctgattacg aaagatcgta tgcgcgtcga tgtagtagtc
     2941 gctttctttg tgcgggtaaa accttcagta gaagggattg ccaccgctgc ccagacgctg
     3001 gggcaacgca ccctgtcgcc tgaagactta cgtatgttgg ttgaagataa atttgtcgat
     3061 gccctccgtg caacagctgc gcaaatgacc atgcatgagt tacaggatac ccgcgagaac
     3121 ttcgtgcagg gagtacaaaa taccgtagct gaagatctgt cgaaaaacgg cctggaactg
     3181 gaaagcgttt cacttaccaa ctttaaccag acctcgaaag aacatttcaa tcctaacaat
     3241 gcctttgacg ccgaaggttt aaccaaactg actcaggaga cggagcgccg tcgccgcgag
     3301 cgtaacgaag ttgaacagga tgtagaagtt gcggtgcgtg agaaaaaccg tgatgcactt
     3361 tcgcgcaagt tgaagattga acaacaagaa gcgtttatga cgcttgagca ggagcagcag
     3421 gttaaaaccc gtaccgctga gcagaatgcg aaaattgcgg cttttgaagc tgaacgtcgt
     3481 cgtgaagcag agcagacgcg aattctggct gaacgacaga ttcaggaaac agaaatcgac
     3541 cgcgaacagg ccgtccgctc aagaaaagtt gaagctgaac gtgaagttcg cattaaagag
     3601 atcgaacagc agcaggtcac cgaaatcgct aaccagacga aatcgatcgc tattgccgcc
     3661 aaatcggaac aacagtccca ggcagaagcg cgtgctaacc ttgcactcgc agaagcagta
     3721 agcgcccaac aaaacgtaga aaccactcgc cagacagccg aagccgatcg tgccaagcag
     3781 gtggcgctga ttgccgcagc acaggatgca gaaaccaaag cggttgaact gaccgtgcgg
     3841 gcgaaagcag aaaaagaagc cgcagagatg caggcggcgg cgatcgttga gttagccgaa
     3901 gctacgcgta aaaaaggcct ggcggaagca gaagcacaac gtgcgctgaa cgatgctatc
     3961 aacgtacttt ctgacgaaca aaccagcctt aaattcaaac tggccttgtt gcaggcgcta
     4021 cctgcggtaa tagagaaatc cgtggaacca atgaagtcca ttgacggtat caagattatt
     4081 caggtcgatg gtctgaatcg tggtggtact gcgggggatg caaacacggg taatgtgggg
     4141 ggcggaaacc tggcggaaca agcattatca gccgcactct cttaccgcac acaggcacca
     4201 ctgattgact ccttgctcaa tgaaattggc gtttcaggcg gctcactggc tgcattgact
     4261 tcactcttaa ccccaacaac tcccgtcgcc gaaaacgtag aataaatttt cagctatgaa
     4321 catccctggc tcaaaaagcc ggggattttt tatatctgcg ttccgctaaa aggtgcaaat
     4381 gatcaggctg ttgcagcgtt ttgcgtgatt gctcggggaa ggcaaaactg cttcagggaa
     4441 agcgttgcgc ggggtccggc gctcatcaac aatcggggag taagcaaagg gctgaaacgg
     4501 gaaagcccct cccgaggaag gggccttaca gaaggaaagg gttatgatga agctcgtcat
     4561 catactgatt gtgttgttac tcgtaagttt cgcagcttat taacagccaa tcagaggggg
     4621 gagaaatcct ccctcaccct tgttccttta ctttaggttg aaaaaaaatc agcgtcaaga
     4681 aggtttcatt aacacatatc cctggcccaa aaagccgggg attttttata tctgcgttcc
     4741 gctaaaaggt gcaaatgctc aggacgttgc agcgttttgc gtgac
//
LOCUS       DBFIFM010000269         4772 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.269, whole genome shotgun sequence.
ACCESSION   DBFIFM010000269 DBFIFM010000000
VERSION     DBFIFM010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.269"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            176..319
                     /locus_tag="N7C74_004410"
     CDS             176..319
                     /locus_tag="N7C74_004410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434141.1"
                     /translation="MNNFSKTSTMQIKRIKPSIIYRLLLIVLGIPMVIYGLVCPLTIE
                     TRD"
     gene            complement(538..2076)
                     /gene="aldB"
                     /locus_tag="N7C74_004411"
     CDS             complement(538..2076)
                     /gene="aldB"
                     /locus_tag="N7C74_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HGU2434142.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKNRYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAEAWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2184..3479)
                     /locus_tag="N7C74_004412"
     CDS             complement(2184..3479)
                     /locus_tag="N7C74_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HGU2434143.1"
                     /translation="MAIKSPPTLIPLSHLSGEELQAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTVRADLRTIVKEGFAQEKKINDQQAVYFAHYPL"
     gene            complement(3609..4760)
                     /gene="yiaY"
                     /locus_tag="N7C74_004413"
     CDS             complement(3609..4760)
                     /gene="yiaY"
                     /locus_tag="N7C74_004413"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HGU2434144.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLAANALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         1158 a         1262 c         1149 g         1203 t
ORIGIN      
        1 tcagagaata taaaaaagaa caattaacgc atattatgaa atgccatgat gcaatgcaat
       61 ttccttattt atataacgac atattgtttt ttatatactt tctgaagtgt gctgcgtggc
      121 tttttaacag gtcgaggctt atcatattga aataatggaa ctatcagcca aactaatgaa
      181 taacttcagt aaaacatcca caatgcagat taagcgtatt aaaccttcaa taatctaccg
      241 tttattgctg attgtcctcg gaataccaat ggtgatttac ggtctggttt gcccgctcac
      301 catcgaaacg cgagattaaa ctcctggcaa aacacgcccc agaaacgcac ttttgttggc
      361 gtagtctggg ttattgcaca cgtaggttta aaacgtaaat tgtaacaacg tgcgctttgt
      421 ttatgccgga tgcggcgtgt acgccttatc cggcctacgg tcaggtcccc ataggcatga
      481 taagacgcgt aagcgtcgca tcaggcaatt aatacccgat gcgaccagct tcttacatca
      541 gaacagcccc aacggtttat ctgagtagct cactagcagg cacttggttt gctggtaatg
      601 ctccagcatc atcttgtggg tttcgcgacc gatacctgat tgtttgtagc caccaaacgc
      661 cgcatgtgcc gggtaagcat gataacagtt ggtccacacg cgcccagcct gtatgccgcg
      721 ccccatctta taggccagat taccgttgcg gctccagacg cccgcgccca ggccatattg
      781 cgtatcgttc gccagctcca gcgcttcttc catcgttttg aaggtagtca ccgccagcac
      841 cgggccaaaa atctcctcct ggaacacgcg catattgttc tgaccaaaca gaatcgtcgg
      901 ttcgaggtag tagccgtctt tcagttcacc ttccagcagc ttgcgccgcc cgccggtgag
      961 cacatcagcg ccctcttttt taccgatatc aatgtagttg aggatggttt ccagttgccc
     1021 gtgagaaacc tgcgcaccca tttgcgtcac gctgtcgagt gggttaccgc tgcgaatgct
     1081 ttcgacacgg cggatggcgc gttccataaa gcgttcgtag attgactcct gcactaaggc
     1141 acgactcgga caggtgcaaa cttcgccctg gttaaaggca aacagtgcaa agccttccag
     1201 cgctttatcg aaaaaggcat cttcttcatc catcacatca gcaaagaaga tatttggcga
     1261 cttaccgccc agctccagcg tcaccggaat aatgttttgc gtggcgtatt gcataatttg
     1321 ttgacccact tccgttgagc cggtaaacgc cactttggcg atgcgtttcg aggtcgccag
     1381 atattcgcca atttccccac ctgcaccgtt gaccacgttc accacgcccg gcggcagtaa
     1441 atcaccgacg atttccatta gcagcagtac agaaagcggg gtaagacgtg cgggtttcag
     1501 caccacacag ttgcccgccg ccagcgcggg agccattttc cagctcgcca tcagcagcgg
     1561 gaagttccac gggataatct gccccaccac gcctaacggt tcatggaaat gataggccac
     1621 ggtttcgcta tcaacttcac tgatcccacc ttcctgcgcc cgaatacacg aggcgaaata
     1681 gcggaaatgg tcaatcgcca gcggtacatc cgcagcactg gtttcgcgaa tgggtttgcc
     1741 gttatcccag gcttcagctg tcgctaacag ctcgaggttt tgttccattc gatcggcaat
     1801 cttaaacaga atcgccgcgc gatcctgcac cgaggtgtgc gcccatttat ctttcacttt
     1861 gtgcgcagca tccagcgcca gatcgatgtc tcgtttgccc gaagacgcca cttcgcacag
     1921 cagctgcccg gtcaccggcg tcaggttctg gtaatactca ccatcggcag gggctaccca
     1981 ttcgccgcca ataaagttgt catagcggtt ttttaacttg agggggaaac catactcgcc
     2041 gggcttaatc tgtgctgaag ggggattatt ggtcatgatc gtctccttgc ggtgtgaggt
     2101 ataacaaggg tagacgtggc tggcgaaatc ttcgccagtc ggtaacagct tttcgacggc
     2161 tatcacgaat ttacgggcaa gggttacagc ggataatgcg cgaagtacac ggcttgctgg
     2221 tcattaattt tcttctcttg cgcaaaccct tctttaacaa ttgtccgtaa atcagctcga
     2281 accgtattat cagagacgcc caggctcgcg ctaatttcag caactgtgta ctctttcccc
     2341 ggattggcga gcatcacatt caggagcgtt acctggcgcg gattcaggcg tttaagcaca
     2401 ccagagctaa acagcacact atcgagtttt gcgcttcgtt gtgtaatttc atcgatatgc
     2461 tgctgccagt tttgcagagc acgtttaatg acacccgcct gatattcaag gaaataagtc
     2521 agatccatac catctgattc agtgtactgg tagctggcgg catatttgac cggtgcagca
     2581 tgaagcaaac ggctgatgga aatatattta aagacatcgt agcccgattt cagcatatac
     2641 cagtaaaaca gcgcccgact ggtgcgcccg ttgccatccc gaaacggatg ttcatgggcc
     2701 agcataaaat gcagaatgca ggcacggacc aacggatgaa tatacccttc atggctgttt
     2761 aaccactggc aaactttttc cagccgttcc ggtaacaatg ccgcagcggg cggttgatgc
     2821 actatattgc catcatagtc ggcgatcaca acatcatctg tttcacgaaa ttctcccgga
     2881 cgatatttcg cgtcattgat tccacccata ccaatggcat gtaactggcg aataagcgca
     2941 ggtgtcagcg gttctgcgag taagtgcgga atttccgcca tcagccgggc atttcccgca
     3001 atcatatgct cgtcttcagt gcgtggcgac cgtcctgttt ccagcatttt tctcgccacc
     3061 agcgttgtag tattcgcacc ttccagctgg gagctggtga tcggctcttc caggcgtaac
     3121 tggcttaact cggctccggc cccgcgtaac cgttcagtct gatctttcag cgccagcgcg
     3181 gtagcacgtt tgtcgacaag ctcgcacgct tctgcaatca ccggggtaat gttgaatccg
     3241 gcctgttcac ccgcttcatt gcaataatta attcgttgga tcgccgcgtt gcgcgccaga
     3301 cgggtaagcg tccacgccac atcgacgttt tctccttttt tgatgcggta ctgcaactca
     3361 tcaaaaggca gataacgtcc tttttcatcg gtaacccggt taaagcgcag gtgggcctgg
     3421 agttcctcac ccgataagtg tgataaagga atcagcgtgg gaggtgattt gatagccatt
     3481 tgcttatctc aaaaaagcgc aatattgaga tagtgtatag caggaaagag aaactatctc
     3541 aaaaaaagca agttttgaga tagttgaaaa aatggctgac aatttgccag ccgttgtgga
     3601 aatgatgatt acatcgctgc gcgataaatc gccacaattt cttcgtgagt tgcctggatc
     3661 gggttagtaa agccacaggc atctttcagg gcattagccg ccagaaccgc gaaatcttct
     3721 tctttcacgt tcaggtcgcg taggcctgcc gggatatcca ctttcttcgc cagttcacgg
     3781 atggcgttaa tgcaggcttc agcaccttcc gcgtcgtttt tacctgtcac gttcacgccc
     3841 attgcagcgg cacagtcacg cagacgtgca gcggcgactt tgctgttgaa tacctgaaca
     3901 tgcggcagca gaacggcgtt acatacaccg tgtggcaggt tgtagaaacc gcccagctgg
     3961 tgcgccatcg catgaacata acccagagaa gcattattga acgccatacc ggcgaggaac
     4021 tgggcataag ccattgcttc acgcgctttc gcattactgc catcttcaac ggctaacggc
     4081 aggttttcgg caatcatggt cacggctttc agtgcacaag cgtcagtgat cggcgtggcg
     4141 gcaatagaaa catacgcttc gatagcgtgc gttaaggcat ccatacccgt tgcggcggtc
     4201 agtgacttcg gcataccaat catcagagag gagtcattga cagaaagcag cggagtgaca
     4261 tgtttatcaa caatcgccat tttgatatga cgcgcttcgt cagtgatgat gcagaaacgg
     4321 gtcatttcag aggccgtacc cgccgtggta ttgatggcga tcatcggcag ctgcggtttt
     4381 gcagagcggt caacgccttc gtaatcgcga atatcgccgc cattggctgc caccagcgca
     4441 ataccttttg cgcagtcgtg tggagaacca ccgcctaagg agatcacgct atcgcaatta
     4501 ttctctttaa gtaatttcaa acctgcggcg acgttttccg tggtggggtt aggttgggtg
     4561 ccatcataaa taacgctaaa aatattgcgt tcttccagtg ctttttgcac atcgcccgcc
     4621 atacctaatt tcgttaacat actgtcagtg acaattaagg tacgggtaaa tccataatct
     4681 gccatcatat tcattgcatc agtcaatgaa tcagcgccga tgacattcac agaaggaata
     4741 aagaaagttg aagatgccat aacacactcc tg
//
LOCUS       DBFIFM010000270         4719 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.270, whole genome shotgun sequence.
ACCESSION   DBFIFM010000270 DBFIFM010000000
VERSION     DBFIFM010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.270"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..525
                     /locus_tag="N7C74_004414"
     CDS             <1..525
                     /locus_tag="N7C74_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2434145.1"
                     /translation="VMGAFASTSTIAAGNNGTARFYGTIEDSPCSIVPDDHKLEVDMG
                     DIGSGILKNNGTSTPKAFQIHLQDCVFDTQTTMTTTFTGNASSTNSGNYYTIYNTDTG
                     AAFNNVSLAIGDAQGTSYKSGAGIEQKIVNDTATNKGKAKQTLDFKAWLVGAADAPDL
                     GNFEANTTFQITYL"
     gene            607..3246
                     /locus_tag="N7C74_004415"
     CDS             607..3246
                     /locus_tag="N7C74_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HGU2434146.1"
                     /translation="MPDHSLFRLRVLPCCVALAMSGSYVNAWAENEIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLTEEYDIYWYASENDASKTYACLTPELV
                     AQFGLKEDVAKNLQWIHDGKCLKPGQLEGIDIKADLSQSALVISLPQAYLEYTDINWD
                     PPSRWDDGISGLIADYSITAQTRHEENGGDDSNEISGNGTVGVNLGAWRLRADWQTDY
                     LHSKSNDDDVINGDDTQKNWEWSRYYAWRALPSLKAKLGLGEDYLNSDIFDGFNYVGG
                     SISTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQLGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDINTASMPFLTRPGQVRYKLMMGRPQEWGHHVEGGFFSGG
                     EASWGIANGWSLYGGALADEHYQSAALGVGRDLSVFGAVAFDITHSHTRLDKETAYGK
                     GSLDGNSFRLSYSKDFDELNSRVTFAGYRFSEENFMTMSEYLDASDSEMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDRDEQTNYNVMLSHYFNLGSIRNMSISMTGYR
                     YEYDNQADKGVYISLSMPWGDSSTISYNGNYGSGSDSSQVGYFSRVDDATHYQLNVGT
                     SDNHSSVDGYYSHDGSLAQVDLSANYHEGQYTSAGISLQGGATLTAQGGALHRTQNMG
                     GTRLLIDADGVAGVPVEGNGAAVYTNMFGKAVVADVNNYYRNQAYIDLNNLPENAEAT
                     QSVVQATLTEGAIGYRKFSVISGQKAMAVLRLQDGSYPPFGAEVKNDSAQNVGLVDDD
                     GNVYLAGVKPGEHMIVSWGGVAHCDIHLPDPLPADLFNGLLLPCQQTGAISPSMPHEI
                     KPVIQEQTQQVMPTEAPVSVSAN"
     gene            3269..4027
                     /locus_tag="N7C74_004416"
     CDS             3269..4027
                     /locus_tag="N7C74_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU2434147.1"
                     /translation="MTNLFCSARTAATTLALLITAASLSVQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKMPYLAQSWMEDGQGNRISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENVSMKNPWQHKV
                     TLTLSGQEFTVNNPTPYFVVISNASAQKNGNPAAGFSPMVISPKTSVPLKVKMDSVPV
                     LTYVNDYGARLPLFFRCTGNTCQVDEEQNSKGQG"
     gene            4038..4538
                     /locus_tag="N7C74_004417"
     CDS             4038..4538
                     /locus_tag="N7C74_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2434148.1"
                     /translation="MKGAYSAWAWLLLCSIVSTPVMAGSKSVAMVLRVLVDAPPPCTV
                     TGASVEFGNVFISKIDGVSYKRPIDYSLVCNNLAMDDLRLNMQATTTVINGETVIDTG
                     IAGFGIRIQKVSDHSILDLTPGAWLPFNFSSGALALEAVPVVQSGVSLTAAEFSASAT
                     IVVDYQ"
     gene            4535..>4719
                     /locus_tag="N7C74_004418"
     CDS             4535..>4719
                     /locus_tag="N7C74_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2434149.1"
                     /translation="MKRISLLMLWSFSFMALSNVSFHGYLVQPPNCSISNGQNIELTF
                     RDVNIDDINGSNYEQVVP"
BASE COUNT         1183 a         1202 c         1270 g         1064 t
ORIGIN      
        1 gtaatgggtg cgttcgcttc tacttcaaca atcgccgctg gcaacaatgg tacagcacgt
       61 ttctacggca ccattgaaga ttccccgtgc tctatcgttc ctgatgatca caaactggaa
      121 gttgatatgg gtgacattgg ttcagggatc ctgaaaaata acgggacttc tacaccgaaa
      181 gctttccaga tccatctgca agactgtgtg tttgacaccc agacaacgat gaccactacc
      241 ttcaccggta acgcgtcttc taccaacagc ggcaattact acaccattta caataccgat
      301 actggtgcgg catttaacaa tgtcagcctg gccattggtg acgctcaggg aacctcttat
      361 aaaagcggcg cgggtatcga acagaaaatc gtaaacgata cggcgaccaa caaaggcaaa
      421 gcgaagcaga cgctggactt taaagcctgg ctggtgggcg ctgctgatgc gccagattta
      481 ggtaattttg aagccaacac gaccttccag attacttatc tctaagtgat acccccatag
      541 ctttcgggct atggggtttt taaatggaat tcgcgcaaat tataataatt acggatggat
      601 acgtgtatgc ctgaccattc tcttttccga ctgcgggtac ttccctgctg cgttgcattg
      661 gcaatgtccg ggagttatgt caatgcctgg gctgaaaatg aaattcagtt tgattcccgt
      721 tttctggagt taaaaggcga cacaaaaatc gatctgaagc gattttccag ccagggttat
      781 gtcgaacctg ggaaatacaa tttacaggtt caactaaata aacagccgct gacggaagaa
      841 tacgatattt actggtacgc ctctgagaac gatgccagta aaacctatgc ctgcctgacg
      901 cctgaactgg tcgcgcagtt tggcttaaaa gaggatgtgg caaaaaacct gcaatggatc
      961 cacgacggca aatgcctgaa acccggtcaa ctggaaggca ttgatattaa agctgacctg
     1021 agtcagtcag cgttagtcat ttcattaccc caggcttacc ttgaatatac cgacatcaac
     1081 tgggatccgc cttcacgctg ggatgacggt atatctggtt taattgctga ctacagtatt
     1141 accgcccaga cacgacatga agaaaatggc ggggatgaca gcaatgaaat tagcggtaac
     1201 gggacggttg gggtgaacct cggcgcatgg cgtcttcgtg ccgactggca gactgattat
     1261 ttgcatagta aaagcaatga tgacgatgtt atcaacggtg atgacacgca aaaaaactgg
     1321 gagtggagcc gctactacgc ctggcgagcc ttaccgtcgc taaaagccaa acttggcctt
     1381 ggcgaagact acctgaattc tgatattttc gacggcttta actacgtggg tggcagtatc
     1441 agcaccgacg atcaaatgtt gccgccgaat ctgcgcggct atgcgccgga tatctccggc
     1501 gtggcgcaca ccaccgcgaa agtgaccgtc agccagttgg gccgcgtcat ctacgaaacc
     1561 caggtcccgg cggggccgtt ccgcatccag gatcttggcg attcggtctc cggtacgctg
     1621 catatccgca ttgaagaaca gaacggtcag gtgcaggaat atgacatcaa caccgcctcg
     1681 atgccgttcc tgactcgccc cggccaggtg cgctataaac tgatgatggg ccgcccgcag
     1741 gagtgggggc accacgtgga aggcggtttc ttctccggcg gcgaagcttc ctgggggatt
     1801 gccaacggct ggtcgctata cggcggggcg ctggcagatg aacactatca gtcggcggcg
     1861 cttggcgtcg gtcgcgacct gtctgtgttt ggtgcggtgg cctttgatat cacccactcg
     1921 catacccgtc tggataaaga gaccgcctac gggaaaggtt cactggacgg caactcgttt
     1981 cgcctgagct attccaaaga cttcgatgaa ctgaacagcc gcgtcacttt tgccggatac
     2041 cgcttctcgg aagagaactt catgaccatg agcgagtatc tcgatgccag cgacagcgaa
     2101 atggtgcgca ccggcaacga caaagagatg tacaccgcca cctataacca gaacttcagg
     2161 gatgccggtg tgtctgttta tctcaactac acccgccata cctactggga tcgcgacgaa
     2221 cagaccaact acaacgtcat gctctcgcac tacttcaacc tgggcagtat ccgcaacatg
     2281 agcatttcca tgaccggata ccgctacgag tatgacaacc aggccgataa aggtgtgtac
     2341 atatcgctca gtatgccgtg gggtgacagc agcaccatca gctataacgg caactacggc
     2401 agcggttcgg acagcagcca ggtggggtat ttcagccgtg tcgatgacgc aacccattac
     2461 cagttgaacg taggcaccag cgacaatcac tccagcgttg acggttatta cagccacgac
     2521 ggatcgctgg cgcaggtcga tctcagcgct aactaccatg aagggcagta cacctcggcg
     2581 ggtatttcct tacagggcgg cgcgacgctc accgcacaag gtggcgcgct ccaccgtacc
     2641 cagaatatgg gcggtacgcg tctgctgatt gatgccgacg gtgtggctgg tgttccggtg
     2701 gaaggaaatg gcgcggcggt ttacaccaat atgttcggta aggcagtggt ggcagacgtc
     2761 aacaactact accgcaacca ggcgtatatc gacctaaaca acctgccgga aaacgccgaa
     2821 gccacccagt ccgtggtgca ggccacgctt accgaagggg ccattggcta ccgtaagttc
     2881 tcggtgatca gcgggcaaaa agcgatggcg gtgctgcgtc tgcaagatgg cagttatccg
     2941 ccgtttggcg cggaagtgaa aaacgacagc gcgcagaacg tcggtctggt tgacgatgac
     3001 ggcaacgtct acctcgcggg cgtaaaacct ggcgagcata tgatcgtttc atggggcggt
     3061 gtggcccact gcgatattca tctgcctgac ccgctgccag ccgatctgtt caatggcctg
     3121 ttattaccat gccagcaaac aggggcgata tctccttcga tgcctcatga aattaagccg
     3181 gtgatccagg agcagaccca gcaggtgatg ccaacggaag cgccagtatc ggtatcagcc
     3241 aattaataac gtgattaagg aatgatccat gacgaatcta ttttgctctg ccagaacggc
     3301 cgcaacgacg ctggcgttac tgataactgc cgccagcctg tcagtacagg cctctgtcac
     3361 gccggaccgt acacgcctga tttttaacga aagtgataaa tcaatcagcg tcacgctgcg
     3421 caataacgac ccgaaaatgc cgtatctggc acaaagctgg atggaagatg gacagggtaa
     3481 caggattagc tcgccactga cggtcctgcc gccagtgcag cgcatcgatt ccatgatgaa
     3541 cggtcaggtg aaggtacagg gcatgccgga tatcaacaag ctgcctgccg accgcgagag
     3601 cgtgttctat ttcaacgtgc gtgagatccc gccgaaatcg aacaaaccta acacgctaca
     3661 gatagcgctg cagacgcgca ttaagttgtt ctggcgacca aaggcgctgg aaaacgtcag
     3721 catgaaaaac ccgtggcagc ataaggtgac cttaaccctc agtggtcagg agtttaccgt
     3781 caataacccg acgccgtact tcgtggttat cagtaacgcc agtgcgcaga aaaatggcaa
     3841 tccggcagcg ggattttccc ccatggtgat atcgccaaaa acctctgtgc cgttaaaagt
     3901 caaaatggat agcgtcccgg tgctgaccta tgtgaatgat tatggtgcgc gtttgccgct
     3961 gttcttccgc tgtacgggaa atacctgtca ggtggacgaa gagcaaaaca gcaaaggcca
     4021 gggataggag caatagcatg aaaggagctt attcggcctg ggcgtggtta ttgctgtgta
     4081 gcattgtgag cacgcctgta atggcgggga gtaaatcggt tgcgatggtc ttgcgtgtgc
     4141 tcgttgatgc cccgccgccc tgtacggtaa cgggtgcgag cgtggagttt ggtaatgtct
     4201 tcatttccaa gatcgatggg gtaagttaca aacgccccat tgactactcg cttgtgtgca
     4261 acaatctggc aatggacgac ctgcgcttaa atatgcaggc aaccacaacc gtaattaacg
     4321 gagaaacggt aatcgatacg ggtattgccg gttttggtat ccgtattcag aaagtaagcg
     4381 atcactccat tttggattta acgcctggcg catggttacc gtttaacttt tcatctggcg
     4441 cactggctct ggaagcggtt cctgtcgtgc agagcggtgt atcgctaacg gcggcggaat
     4501 ttagcgccag tgccacaatt gtggtggatt atcaatgaaa cggatctctc tcttaatgtt
     4561 gtggagcttt agctttatgg cgctctcgaa tgtctctttt catggttatt tagtgcagcc
     4621 acccaattgc tctattagta atgggcaaaa tatagagttg acgtttcggg acgtgaatat
     4681 tgatgacatt aacggtagca attacgaaca ggtcgtgcc
//
LOCUS       DBFIFM010000271         4693 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.271, whole genome shotgun sequence.
ACCESSION   DBFIFM010000271 DBFIFM010000000
VERSION     DBFIFM010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.271"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            184..1341
                     /gene="acrE"
                     /locus_tag="N7C74_004419"
     CDS             184..1341
                     /gene="acrE"
                     /locus_tag="N7C74_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HGU2434150.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEVR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1353..4502
                     /gene="acrF"
                     /locus_tag="N7C74_004420"
                     /pseudo
     CDS             1353..4502
                     /gene="acrF"
                     /locus_tag="N7C74_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
BASE COUNT         1146 a         1138 c         1210 g         1199 t
ORIGIN      
        1 aataaaacag tagaatatta atcttttttt gtgtttatgt gccttgagac gcctgtattc
       61 ataactattc cttacatcga cgaatgataa tttgtaggat agcgaactgt atttttcttt
      121 ctgcgagtta acgcgttgcc tttttgggta aataacgcgc ttttggtttt ttgaggaata
      181 gtaatgacga aacatgccag gtttttcctc ctgccctcct ttattctgat ctccgcggct
      241 ttaatcgccg gttgtaacga taagggagaa gagaaagctc acgtcggtga accgcaggtt
      301 accgttcata ttgtaaaaac ggccccgtta gaagttaaga ctgaattacc aggccgcacc
      361 aatgcttatc gtatagccga agttcgccca caggttagcg ggatcgtact gaatcgcaat
      421 ttcactgaag gcagcgatgt gcaagcaggc cagtccctgt accagatcga tcccgcgacc
      481 tatcaggcaa attatgacag cgcgaaaggc gaactggcga aaagtgaagc cgccgccgcc
      541 atcgcgcatt tgacggtaaa acgttacgtt ccgctcgtgg gtacgaaata catcagccag
      601 caggagtacg accaggccat tgctgatgct cgccaggccg atgccgccgt gattgccgca
      661 aaagccacag tcgaaagcgc tcgcatcaat cttgcttata ccaaagtgac tgcgccaatt
      721 agcggacgta tcggcaaatc gactgtgacc gaaggcgctc ttgtcactaa tgggcaaacg
      781 actgaactgg caactgtcca gcagctcgat cctatctatg ttgatgtgac ccaatccagc
      841 aacgatttta tgaggctgaa gcagtccgta gagcaaggaa atttgcataa ggaaaacgcc
      901 accagcaacg tagagttggt catggaaaac ggtcaaacct atcccctgaa aggtacgctg
      961 caattctccg atgtgaccgt tgatgaaagc accggctcca taaccctacg tgctgtcttc
     1021 cctaacccgc aacatacgct tttgccgggt atgtttgtgc gtgcacggat tgatgaaggc
     1081 gtccaacctg acgccattct catcccgcaa caaggcgtta gccgcacacc gcgtggtgat
     1141 gcaaccgtgc tgattgttaa cgataaaagt caggtggaag tgcgccctgt cgttgccagt
     1201 caggcgattg gcgataaatg gttgattagc gaaggtctga aatctggcga tcaagtcatt
     1261 gtcagcggcc tgcaaaaagc gcgtccggga gagcaggtta aagccactac cgataccccc
     1321 gcagatactg catcgaagta aggtaatctg acatggcaaa cttttttatt cgacgaccga
     1381 tatttgcatg ggtgctggcc attattctga tgatggcggg cgcactggcg atcctacaat
     1441 tgcccgtcgc tcagtatcca accattgcac cgcctgcggt ttctgtttca gcaaactatc
     1501 cgggcgctga tgcgcagacc gtgcaggata cggtgacgca ggttatcgaa cagaatatga
     1561 acggtatcga taacctgatg tatatgtcct ccaccagcga ttccgccggt agcgtgacaa
     1621 ttacccttac cttccagtcc gggaccgatc ctgatatcgc gcaagtgcag gtgcagaaca
     1681 aactccagct cgccacgccg ttgctgccgc aggaggttca gcagcagggg atcagtgttg
     1741 aaaagtccag tagcagctat ttgatggtgg caggctttgt ctctgataac ccagacacca
     1801 cacaggacga tatctcggac tatgtggcct ctaacgttaa agatacgctt cgccgtctga
     1861 atagcgtcgg tgacgtacag cttttcggcg cacagtatgc gatgcgtatc tggctggacg
     1921 ccgatctgct aaacaaatat aaactgacac cggttgatgt gattaaccag ttgaaggtac
     1981 agaacgctca gatcgctgcc ggacagttgg gcggaacgcc agcgttacca gggcaacagt
     2041 tgaatgcctc gattattgct cagacgcggt taaaaaatcc ggaagaattc ggcaaagtga
     2101 ccctgcgcgt aaacagtgac ggctcggtgg tgcgtctgaa agatgtcgca cgggttgaac
     2161 ttggcggtga aaactataac gttatcgctc gcatcaacgg aaaaccggcg gcgggcctgg
     2221 gtattaagct ggcaaccggc gcgaatgctc tcgataccgc gaaagccatt aaggcaaaac
     2281 tggcggaatt acagccattc ttcccgcagg gaatgaaggt tctctaccct tatgacacca
     2341 cgccattcgt ccagctttct attcacgaag tggtaaaaac actgttcgaa gctattatgc
     2401 tggtgttcct ggtgatgtat ctgttcttgc agaatatgcg agcaacgctg atccccacca
     2461 ttgcggtacc cgtggtgttg ttagggacgt ttgccatcct cgccgctttt ggttactcca
     2521 tcaacacact aacgatgttc gggatggtgc ttgccatcgg gctgctcgtc gatgatgcga
     2581 tagtggtggt ggagaacgtc gagcgcgtga tgatggagga taagctcccg ccaaaagaag
     2641 cgacggaaaa atcaatgtcg caaatacagg gcgcactggt gggtatcgcg atggtgctgt
     2701 cagcggtatt tattccgatg gcattcttcg gcggttctac tggggcaatt tatcgtcagt
     2761 tctctatcac catcgtttcg gcaatggcgc tttctgttct ggtggcattg attcttaccc
     2821 ctgcgttatg tgcaacactg ctcaaaccca cctcagctga gcatcacgaa aataagggtg
     2881 gtttcttcgg ctggtttaat accaccttcg atcatagcgt taaccactac accaacagcg
     2941 tcggcaaaat cctcggttcc acaggacgat atttactgat ctatgcgctg attgttgcag
     3001 gaatggtggt gttgttttta cgtcttccgt cttccttctt acctgaagag gatcagggtg
     3061 tctttctgac catgattcag ttacccgctg gcgcgacgca agagcggacg caaaaagtgt
     3121 tggatcaagt gacggattac tatctgaaga acgagaaagc aaacgttgaa agtatcttta
     3181 cggttaacgg ctttagcttc agcggccagg cacctccgca accccatgtt ttcacataac
     3241 tgttgcgttg accaattgaa tctacaggta tggccttcgt cagtctgaaa ccgtgggaag
     3301 agcgtaatgg tgatgaaaac agtgcggaag cggtaatcca tcgtgccaaa atggagttgg
     3361 gcaagatccg cgacggtttt gtcattccat tcaatatgcc agccattgtt gaactgggca
     3421 cggcaacggg tttcgacttt gagttaattg ataaggctgg gctgggtcac gatgccctaa
     3481 cccaggcccg taaccagttg cttggtatgg cggcgcaaca tcctgccagc ttagtcagcg
     3541 tgcgtcccaa tggcctggaa gacaccgcgc agtttaaact ggaagttgac caggaaaagg
     3601 cgcaggcatt aggtgtttca ctttctgaca tcaatcagac catttcaacg gcgttgggtg
     3661 ggacttacgt taacgacttc atcgaccgtg gtcgcgtgaa aaaggtgtat gttcaggcgg
     3721 atgccaaatt ccgtatgctg ccagaagatg tcgataaact ttatgtccgc agcaccaacg
     3781 gcgaaatggt gccattctct gcctttacca cttcacattg ggtgtatggc tctccgcgac
     3841 tggaacgcta caacggtctg ccgtcaatgg agattcaggg ggaagccgcg ccaggaacca
     3901 gttccggcga tgccatggcg ttgatggaaa acctagcgtc aaaattacct gcgggcattg
     3961 gttatgactg gacgggtatg tcgtatcagg aacgcttatc gggaaaccag gctcccgctc
     4021 tggtagcaat ttcctttgtg gttgttttcc tgtgccttgc tgcactctat gaaagctggt
     4081 caattcctgt ctcggttatg ttggtcgtgc cgttagggat tgtcggcgtg ctgctggcgg
     4141 cgacactctt taatcaaaaa aatgacgtct actttatggt aggcttgcta acgacaattg
     4201 gcttgtcggc caaaaacgct attttgattg ttgagttcgc taaagatctc atggagaaag
     4261 agggtaaagg tgttgttgaa gcgacactaa tggcagtacg tatgcgtctg cgtcctatcc
     4321 tgatgacctc tctcgccttt attctcggcg tattaccgct ggctatcagt aacggtgccg
     4381 gcagtggcgc gcagaacgca gtgggtatcg gggtaatggg aggaatggtc tctgcaacgt
     4441 tgctggcaat cttcttcgta ccggtgttct ttgtggtgat ccgccgttgc tttaaaggat
     4501 aaatctgaaa cataaaggcg ctttcgggtg cctttatgtt ttccagtgaa acccataaaa
     4561 attaataaga tattcttttg ctcactcttt aaaagctttc tatagctccc gctcccttca
     4621 ctatttttac aattcacata atttgtcatt attcattccg caagatttat aacctccggt
     4681 gataaaatgg cat
//
LOCUS       DBFIFM010000272         4686 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.272, whole genome shotgun sequence.
ACCESSION   DBFIFM010000272 DBFIFM010000000
VERSION     DBFIFM010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.272"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..513)
                     /locus_tag="N7C74_004421"
     CDS             complement(<1..513)
                     /locus_tag="N7C74_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HGU2434151.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCSPEHGTKLALERRNKERE
                     KAEKTAEKKRRREEQKQKDKIKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQE
                     AVDEIESNHNR"
     gene            complement(513..1235)
                     /locus_tag="N7C74_004422"
     CDS             complement(513..1235)
                     /locus_tag="N7C74_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HGU2434152.1"
                     /translation="MNELINSNAIKMTSIEIAELVGSQHGNVRISIERLAKRGVIQLP
                     SMQKVENKQTISPNKFTSVYIFEGEQGKRDSIIVVAQLSPEFTARLVDRWRELEGATA
                     KIPQTFSEALRLAADLEDQKAELEKQLALAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKRFAWMRDHKILIASGARRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            complement(1310..1990)
                     /locus_tag="N7C74_004423"
     CDS             complement(1310..1990)
                     /locus_tag="N7C74_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HGU2434153.1"
                     /translation="MKTISVESLSIISFSNIPVVTTELLASLYGTEPDYIRKNFNRNS
                     GRFVIGKHYFLLENEELREFKHSMSLRPSVKIARNVRSLILWTERGAARHAKMLETDR
                     AWEVFEKLEDCYFSQCEKNTGKQEKKLNGLSAKETDSLVWLWDYANRSQALFRELYPA
                     LKLIQSGYSGICHDYGYEFSYIIGRARGVLINHTRDIDIYEPDGPTNLLAWERLKNKE
                     LPPSLHRY"
     gene            complement(2246..3004)
                     /locus_tag="N7C74_004424"
     CDS             complement(2246..3004)
                     /locus_tag="N7C74_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HGU2434154.1"
                     /translation="MTTKISVETLSPITHNQIPVITTELLAQLYGTEPVRIRQNHHEN
                     KVRFVEGKHFFKVVGNDLKELRVALNYSQNLRVTLSNSQNLRVTLSNSQNLQPSLRGL
                     QISPKARSLILWTERGAARHAKMLETDRAWEVFEKLEDCYFSQKIPEQLQLPESTLSI
                     NYPLSWFSEHHPYSMMSYVDRKTLNLDVSVLFDMPSPTMRILNELHSKGYNVDAAVAE
                     FNAFKHLTEEMRRTLQDISRLSDRNSRKGFSLSL"
     gene            complement(3279..3461)
                     /locus_tag="N7C74_004425"
     CDS             complement(3279..3461)
                     /locus_tag="N7C74_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGU2434155.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
     gene            complement(3458..3985)
                     /locus_tag="N7C74_004426"
     CDS             complement(3458..3985)
                     /locus_tag="N7C74_004426"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HGU2434156.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWISHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGLGVRRAA"
     gene            complement(3982..4428)
                     /locus_tag="N7C74_004427"
     CDS             complement(3982..4428)
                     /locus_tag="N7C74_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HGU2434157.1"
                     /translation="MKQTFLLRNEAIRNNAIDAILSLPIDDKSPHEVHVKEPKRSKAQ
                     NDRMWPMLNDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSAPGIDGGVVMLGV
                     RTSKMRKASMTELIEIMFWFGSERNVRWSDDSRREYEWSQRKGKAA"
     gene            complement(4385..4621)
                     /locus_tag="N7C74_004428"
     CDS             complement(4385..4621)
                     /locus_tag="N7C74_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
                     /protein_id="HGU2434158.1"
                     /translation="MSDLSLTQPKLKECPFCGGNARLWVEAGINIDVWGYAECDLCEA
                     RVAWAPSVAAATEKWNRRAGDEANLSASQRSNQK"
BASE COUNT         1050 a         1166 c         1103 g         1367 t
ORIGIN      
        1 gcggttatgg tttgattcga tttcgtctac tgcttcctgc ccgatgcggt taatcagttc
       61 gacgcgatac ggaacgagat ttccgctttt atgttggttg cacaccacgc attgcttgtg
      121 aatattgcgt tcatcaaatc ggagttgagg cgccgcagca gttgtccggt aatgtccggc
      181 atcccactga gcagacgtga gcgttccgca cgagatacat ggtaagtcgc ggtctctttc
      241 tctgatgaag gcgtttacgg cttgttgggc ttgtttaatc cagtaactgc ggggctttaa
      301 ggcgagtttt cgaatcttaa ttttatcttt ctgtttctgc tcctctcgtc gtcgtttctt
      361 ctctgctgtt ttttccgcct tttcgcgttc tttatttcgt cgttcgagtg ctaatttagt
      421 tccgtgttcc gggctgcacc accactgatt tgagaatgcc gggtgaaacc attccttaca
      481 gattttgcat ttccttcgcg ctggtttagc cattaagcag cctcccctgt tactttaagc
      541 attccgttat ctagcagctt tcttgtcagc cactgttgac cacgcccggt gatttttgtg
      601 gtgaacgata tctgtattcc gtgatttgta ttgaccgctg tttctttcac tgtgaaatag
      661 ccgcgatcca tatattcctg cattggcaca ttgcgccggg cacctgaagc aataaggatt
      721 ttgtgatcgc gcatccacgc aaaccgtttg tttggaccaa taccaacaac ctttgcaaag
      781 tttccaatca aaattccgct ggcctcgcca actcgatcgg caaactcaac tttaggtgct
      841 gcgagagcaa gctgtttctc cagttcagcc ttctggtctt caaggtcggc cgcaaggcgc
      901 aatgcctcag aaaaggtttg tggtattttc gcggttgccc cttcgagttc tcgccagcgg
      961 tcaacaaggc gagcggtgaa ttccggcgac aactgagcga cgacaataat gctgtctcgc
     1021 ttaccttgtt cgccttcgaa tatatacacg cttgtgaatt tgttagggct aattgtttgt
     1081 ttattttcaa ctttttgcat tgaaggaagt tgaatcaccc cacgctttgc cagacgttct
     1141 attgatattc tgacattacc gtgttggctt cccaccaact cagcgatttc aatgcttgtc
     1201 attttgatgg cattgctatt tatcagctca ttcattgtca tgtcctctca cattgaaaat
     1261 tcagcaataa aaaacccagc cgaagctggg tttgttaagt tgtcaattgt cagtagcgat
     1321 gcagtgaagg cggcaactct ttgttcttaa gcctttccca tgccagaagg ttcgtcggcc
     1381 cgtcaggctc ataaatatct atatcccgcg tgtgattaat taaaacgccc ctcgccctcc
     1441 cgatgatata cgagaactca tagccgtagt cgtggcatat gccggaatag ccagactgaa
     1501 tcagttttaa tgcgggatac aactcacgga acaatgcctg tgagcggttg gcataatccc
     1561 acagccatac aaggctgtct gtttcttttg cggaaagccc gttgagcttc ttctcttgtt
     1621 tgccagtatt tttctcgcac tggctgaaat agcagtcttc cagtttttcg aacacttccc
     1681 acgcccgatc ggtttcgagc atttttgcgt gacgggctgc gccgcgttct gtccagagga
     1741 tgagggagcg aacgttacgg gcgattttca cagaaggtct taaagacatg ctgtgcttaa
     1801 attcgcgcaa ctcctcattc tcaagtaaaa agtagtgctt accgataaca aatcgtccag
     1861 aattccgatt gaaatttttt cggatgtaat ctggttctgt gccatataag ctggcaagaa
     1921 gctctgtagt caccacaggt atattactaa atgaaattat ggagagggac tccacggaga
     1981 tggttttcat gctttatgtc ctacttgata ttttgattta ccccttttga gagggtgacc
     2041 gggcgctcaa aaccgcaagt agtcggcggg catattccca ttgctggtgt tgtattagcc
     2101 gcacgcccgg tcataaacca agaagactgg acacaaaaaa accgcatgtc tgtcgggcgc
     2161 ggtaaccgct acttgctaag gtgttttgag caccatgagg cggaatatat ccccgttaat
     2221 gcagcattgt caaacgatct gttctttaaa gacttaacga gaagcctttt cgggaatttc
     2281 gatctgacaa tcttgaaata tcctgtagag tacggcgcat ttcttccgtc agatgcttga
     2341 aggcgttaaa ttcggcgaca gctgcatcga cgttatagcc cttgctgtgt agctcgttga
     2401 ggatgcgcat agttgggctt ggcatatcga agagcacaga aacgtccagg ttaagagttt
     2461 ttcgatcaac atagctcatc atggagtagg ggtgatgctc tgaaaaccac gagagagggt
     2521 agttgataga tagtgttgat tctggaagct gaagttgttc tgggattttt tgactgaaat
     2581 agcagtcttc cagtttttcg aacacttccc acgcccgatc ggtttcgagc atttttgcgt
     2641 gacgggctgc gccgcgttct gtccagagga tgagggagcg ggctttcggg gaaatttgta
     2701 accctcttaa agatggttgc aaattttgtg agttacttaa agtaacccgc aaattttgtg
     2761 agttacttaa agtaacccgc aaattttgtg agtagtttaa agctacccgc aattctttaa
     2821 ggtcattacc aacaactttg aaaaagtgtt tcccttcaac gaagcgtact ttgttctcat
     2881 gatgattctg gcgaatacgc accggctcag tgccgtaaag ctgcgccaaa agttcggtgg
     2941 taataacagg aatctggtta tgggtgatcg gggagagagt ttcaacagaa attttagttg
     3001 tcataatgac gccctctggt ggtttcttaa taactcacca ccgacgacgc caatcgtctg
     3061 gtggtgaact gtgcagggtt ggcgtaaccg ggaaaccgac cggcgcggat ctctccgccc
     3121 ccacacagcc caccataatt cagatgtgcg cgtgcatacg acaataaaaa acacgctcgc
     3181 ggcgtgtatc tgtcgcggtc tctatccagg acgccaatcc cgacgccaga ttttgctggc
     3241 gtttgaggaa tatagccccg aataaatcat cacgtcaatc accttgtttt cctcgcacga
     3301 tgtcttagcc accggatatc ccacaggtga gccgtgtaat tgaaggtttt tacgtcagat
     3361 tcttttggga ttggcttgcg tttatttctg gagcgtttcg ttggaaggta tttgcagttt
     3421 tcgcagatga tgtcggtgat acttcgtcgc tgtcgcctca tgcagccctc ctgacgccca
     3481 gcccgatcgc catcaatgcc gctttggata cggtagtaaa catccgtcga ggactgatga
     3541 acggtcgcca aatcagcaac attgagcctt tgctgtttcc cttcttctcc agccctgtcg
     3601 atggttcgat aaaattaatc cgtccatcag tgatgatgcg aacttcgtca acactctcca
     3661 gagccttgct gaaccatccg acagacatat cctctggcac aagcatcact accgtctgtc
     3721 gctgttgtat gcactgctca gcggcttttt ccacccacgg cctgatattg ctgtacggtg
     3781 ggttattcca gattgcaccg tggcttatcc actcagaatt tagcgcgtcg tcagcctcag
     3841 ttagccagtg agcgcacaga gcgtttttgt cgctcgcagc tgaatccagc cagaatccaa
     3901 actcaatatc cagcgcatca aaaagccaga gcggcgtttg ccagcagtcc ttgtcgtgtg
     3961 ctggcgtatt tgatttgata gtcatgcagc cttccctttt cgttgtgacc attcatactc
     4021 tcgccgggag tcatcactcc accgcacgtt gcgctctgag ccgaaccaga acatgatttc
     4081 gataagctca gtcatgctgg cctttcgcat tttgctggta cgcacgccaa gcatgacaac
     4141 gccaccgtcg ataccaggcg cacttctttg ctccagtttt ttggtcttaa gccacagggc
     4201 agtgaacagg tctttccagt cttccggcgc cagccgttga ccatgccata gcacctgacg
     4261 cgaaacatcg ttcagcatcg gccacatgcg gtcattctgc gctttgctgc gcttgggttc
     4321 tttaacgtgg acttcgtggg gtgacttgtc gtcgatgggt agtgagagaa tggcgtctat
     4381 ggcgttattt ctgattgctt cgttgcgaag cagaaaggtt tgcttcatct cctgctctcc
     4441 ggttccattt ttcagtagcc gcagcaactg atggtgccca tgccaccctg gcttcacaga
     4501 ggtcacattc tgcatagccc cacacatcaa tatttattcc ggcctcaacc cacagacgag
     4561 cattaccgcc gcaaaacgga cattctttta gctttggctg ggttaatgat aggtcgctca
     4621 tgctcactcc ttcacttaaa atccagactc cggataattc tgttgcgctg aaactcattg
     4681 ttgagt
//
LOCUS       DBFIFM010000273         4621 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.273, whole genome shotgun sequence.
ACCESSION   DBFIFM010000273 DBFIFM010000000
VERSION     DBFIFM010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.273"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            5..2476
                     /locus_tag="N7C74_004429"
     CDS             5..2476
                     /locus_tag="N7C74_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGU2434159.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEGAASFVPVETESDPM
                     NVAFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RVHRQHTENSAEKIPPVTAVIRREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQSATDTLSSPVPETDAVETAIPDNEKTECKVEVEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNANTAQNSEQ
                     PEPVKVTADEVKKIMQAANISQPDANQLLAASRGEFVAGISDPNDPKWVKGIETRDSV
                     NQNQQETEQNDQKAEQNSPNTQQNEPETKQPEPVAQQEPEKVCTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTDGNQHHDSDNETGETADHS
                     IKVNGHQEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSVIWQKLIPNQADF"
     gene            2541..2789
                     /locus_tag="N7C74_004430"
     CDS             2541..2789
                     /locus_tag="N7C74_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU2434160.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            2767..3897
                     /locus_tag="N7C74_004431"
     CDS             2767..3897
                     /locus_tag="N7C74_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HGU2434161.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(3943..4581)
                     /gene="ompW"
                     /locus_tag="N7C74_004432"
     CDS             complement(3943..4581)
                     /gene="ompW"
                     /locus_tag="N7C74_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HGU2434162.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
BASE COUNT         1378 a         1110 c         1141 g          992 t
ORIGIN      
        1 caaaatgagt aaagtcttta tttgcgccgc cattccggac gaacaggcaa taaaggaaga
       61 aggtgcagtc gctgtagcca ctgccattga agccggtgat gaacgtcgcg cccgcgcaaa
      121 atttcactgg caattcctgg agcattatcc ggctgctcag gactgcgctt ataaatttct
      181 tgtttgcgag gataaacccg gtataccccg ccctgccctc gattcctggg atgctgaata
      241 tatgcaggaa aaccgctggg atgagggggc tgcttccttt gtcccggttg agactgaatc
      301 cgatccgatg aacgtcgctt ttgacaagct ggcccctgaa gtacagaacg ctgtcatggt
      361 taagttcgac acatgtgaaa acatcaccgt tgatatggtg attagcgcgc aggaattgtt
      421 gcaggaagac atggcaacat tcgacggaca tatcgttgaa gcgttgatga aaatgccaga
      481 agttaacgcc atgtatccgg agcttaagct gcatgccatc gggtgggtta agcataaatg
      541 taagcctggt gccaaatggc ccgaaattca ggcagaaatg cgcatctgga aaaaacgtcg
      601 cgaaggtgaa cgcaaggaaa ccggaaaata cacgtctgtt gttgatctcg cccgcgccag
      661 agtccaccga cagcacactg aaaactcagc agaaaaaatc ccccctgtca ctgcagtcat
      721 tcgtcgcgaa tataagcaga catggaaaac actggatgac gaactggcct acgctctctg
      781 gcctggtgat gtggatgccg gaaacattga cggcagcatc catcgctggg caaaaaatga
      841 agttatcgac aacgaccgcg aagactggaa gcgtatctcg gcatcaatgc gcaaacagcc
      901 tgatgccctt cgctacgacc gccagactat ttttggcctt gtccgtgaac gtccgatcga
      961 cattcacaaa gaccctgtgg cactgaacaa atacattact gaatacctga ctacaaaggg
     1021 cgtgtttgaa gatgaaggaa caaatcagag cgcaactgac actctctcgt cgccagtacc
     1081 agaaactgat gcagtggaaa cggcaattcc ggacaacgaa aaaaccgaat gcaaagtgga
     1141 agtcgaacca tctgtagagc gtgaggggcc gttctacttc ctcttcaccg acaaggatgg
     1201 cgaaaaatac ggtcgcgcaa acaaactttc tggtctggat aaggcgctgg ctgccggggc
     1261 tactgaaatc acaaaagaag aatattttgc ccgaaaaaat ggcacataca caggcttacc
     1321 gcaaaatgca aataccgcac aaaattctga acaaccagaa ccggtaaaag ttaccgctga
     1381 cgaagtaaag aaaattatgc aggcagccaa tatcagccag cctgacgcca atcagttgct
     1441 cgccgcatca cgtggtgaat ttgttgcagg gattagcgac ccgaatgatc cgaaatgggt
     1501 gaaggggatt gaaacccgcg attctgtgaa ccagaaccag caagaaacgg aacagaacga
     1561 ccagaaagcg gaacaaaaca gcccaaatac gcaacaaaac gagccagaaa cgaaacaacc
     1621 tgagccagta gcgcaacagg aaccggaaaa agtctgcacc gcctgcggtc agaccggcgg
     1681 cggcaactgc cctgattgtg gcgcggtgat gggcgacgca acataccagg aaacattcga
     1741 tgaagagtat caggttgaag ttcaggaaga tgatccggag gaaatggaag gcgctgaaca
     1801 tccacacaag gagaacactg acggcaatca gcatcacgat agcgataatg aaactggcga
     1861 gacggcagat cactcaatta aggtgaacgg tcatcaagaa atcacatcca ccagcaggac
     1921 gtgtgaccat ctaatgatcg accttgaaac catgggaaaa aatcctgatg ccccgatcat
     1981 ctcaataggt gcaatatttt tcgatccgca aaccggagat atgggaccgg aatttagtaa
     2041 gactatcgat ctggaaactg ctggcggggt cattgatcgg gacaccatta aatggtggct
     2101 taagcaatca cgcgaagcgc aatctgccat tatgaccgat gaaatcccgt tagatgatgc
     2161 actattacaa ttgcgggaat ttatcgacga aaactccggt gaattttttg ttcaggtctg
     2221 gggaaatgga gccaacttcg acaacacgat tttgcgccgt tcatacgaac ggcaggggat
     2281 cccctgcccg tggcgttact acaacgatcg cgatgtacgc acaatcgttg agctggggaa
     2341 agccatagac ttcgatgcca gaacggctat tccattcgaa ggtgagcgcc ataatgcact
     2401 tgatgacgcc cgttaccagg caaaatacgt ttcagttatc tggcaaaaac tgatcccgaa
     2461 tcaggctgat ttttaatgtt caaccccggt cgttgcccac cagctatagt ggcggcgacc
     2521 atgattagcg aacgacgctc atggcaagac ttattctgct cactgagtgg gcaaaagagg
     2581 aattcagtga accggtccca actccgagta cgttaagtaa atacgctaaa gccggaatga
     2641 tatttcctct ccccaaaaaa gttggaagac gctggcgagt ggatccgcaa gctcgctttg
     2701 tcggaatggt aaacaagccg gaggtgatcg ccacagatca ccctgctttg aagaggatac
     2761 tggaagatgg cgcgcccgcg aaaatataaa accgatgttc cgggattatc tccgtatttt
     2821 gacaaaagaa ataacaaagt ttactggcgt tacaggcatc ccataacagg caaaaatcac
     2881 ggtctcggca gtattgacca gaaactggca gaaactattg cagcagaagc gaacagccgt
     2941 cttgcccggc agcaaatgga acaaatgctc agtctgcagg agaaaattat tagtgatacc
     3001 ggcggttcat caaccgttac catttttctg aataattaca gaaaaattca acaggaaaga
     3061 tatgaaaacg gcgagatcaa actcaacacg ctgaaacaga aagcggcccc tctcagggta
     3121 tttgatgaac gttttggcac cagaccgtta gatgccataa ccgtaaaaga tgtggtatca
     3181 gtactggaag agtacaaggc cagaggacat aacagaatgg gacaaatttt caggaaagta
     3241 ctgatcgatg ttttccggga agctcagcaa acgggcgatg tcccgccagg ctttaaccct
     3301 gcagaatcgg caaaaaaacc gcaggtgcgg atatcaagac agcgactgac ttttgatgag
     3361 tggatgatga tttataacgc agcggaaaag gatggttact ttttacagcg cggtatgctg
     3421 ctggcactga tgacaggcca gcgcctttca gatatttgca aaatgcaatt ttcggatatc
     3481 cgggatggtt atcttcatgt cgaacagcaa aaaacaggaa cccggattgc catccctctg
     3541 gctctgcgtt gcgataaatt aaatctcacc ctggatgatg tggtgtcatc ctgccgcgat
     3601 tgcgttctta gtccgtggct attgcaccac catcacgcga aagggacagc taagcgcggc
     3661 gggatggtta agccagcaac attaaccgtt gcatttaaaa aagcccggga ttctgtggat
     3721 tacaactggc gtgctaatgg cacccctccc tctttccatg agcagagatc tttatcagag
     3781 cgattgttca gagagcaggg ggttgatacc aaaattttgc taggccattc gaatcaaaaa
     3841 atgaccgata tttacaacga cgcacgcggt aaggaatgga aaaaactggt catttgatga
     3901 ccagttttgc agaggggttt tgcagaggtt ttgcagagaa aattaaaaac gatatcctgc
     3961 ggagaacata aacacccacg gatccagtcg taccgagtct tttacggtag taacaccaga
     4021 tttgtactta gccgtggtat cgatatccat gtaccacact gacatgttaa ccaaccagtc
     4081 acggttaatc agataatcaa cccccacctg cccggcagct ccccaggaat ctttcagact
     4141 gagatcggaa agccccgcct ctttgccatg atcgttaaat ccattatcaa agaaggtggt
     4201 gtagttaata cctgccccaa cataaggacg gaatttgctg ctggcatcac caaaatacca
     4261 ctgcgccatc agtgttggcg gcagatgatg aacggttgca atatcgccgg tcgcccgggt
     4321 gccgatttta tggcggaacg gcgtcgctgc cagtaattcc acaccaatgt tgtcggtcgc
     4381 catataagta aacgtcaggc ccagttgagt gttattggtc acgctgaatc cacccagact
     4441 tcctaacgta ccaccagcac cttctgttgg acgtacggtt gcagaacctg cacgcataaa
     4501 aaattcgcct gcttcatgcg caaaggcact gccagagaga agagttgtta ctgccaaagc
     4561 cgccactgtt aactttttca tatccgctcc gtcgttatgg ttataaaaac gtgacgaata
     4621 t
//
LOCUS       DBFIFM010000274         4621 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.274, whole genome shotgun sequence.
ACCESSION   DBFIFM010000274 DBFIFM010000000
VERSION     DBFIFM010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.274"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..2630
                     /locus_tag="N7C74_004433"
     CDS             <1..2630
                     /locus_tag="N7C74_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU2434163.1"
                     /translation="KAYRMHQVLLLPRFARLTIALSLATAVFPVDAEYYFNPRFLSND
                     LAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFITDDNNADLIPCLSTDLLV
                     SLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLVDASTEFDVGQQHLSLSVPQIYVG
                     RMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQSGINIGSWR
                     LRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGDIFDSVNFR
                     GLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGPFTIDDINS
                     AANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGNNLQSSPRF
                     IQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQANTTLADGTR
                     HSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVKPPTGDSNE
                     QTQFIDYFNLFYSKRGQEQISISQQLGNYGATFFSASRQSYWNTSRSDQQISFGLNVP
                     FGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNASYSMSNDL
                     KGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSTLNYRGAYGNTNVGYS
                     RSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQTGIHTDWRG
                     YAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQIGGKVLMT
                     LKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDKNSNCIVDY
                     KLPEVSPGTLLNQQTAICR"
     gene            2644..3174
                     /locus_tag="N7C74_004434"
     CDS             2644..3174
                     /locus_tag="N7C74_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2434164.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            3187..3690
                     /locus_tag="N7C74_004435"
     CDS             3187..3690
                     /locus_tag="N7C74_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2434165.1"
                     /translation="MKRLHKRFLLATFCALLTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPAETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            3750..>4621
                     /locus_tag="N7C74_004436"
     CDS             3750..>4621
                     /locus_tag="N7C74_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2434166.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGTTTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAG"
BASE COUNT         1313 a          997 c         1055 g         1256 t
ORIGIN      
        1 ttaaggcata tcgtatgcat caggtgttac tactgcctcg ctttgcacgg cttaccattg
       61 cccttagctt agcaactgcg gtatttcccg ttgacgcaga gtattatttt aatccacggt
      121 ttttaagcaa tgatcttgct gaatctgtcg atttatccgc ctttactaag ggacgcgagg
      181 caccacctgg tacatatcgg gtcgacattt atttgaacga cgaatttatg actagtcggg
      241 atattacttt tattacagat gataataacg cagatctcat cccatgcctg agtacagatc
      301 ttcttgtcag tcttggcata aaaaaatctg cgttattaga caataaggaa cattcagcag
      361 aaaaacatgt gccggacaac agcgcatgca caccacttca ggatcgtttg gtcgacgcat
      421 caactgagtt tgatgttggt cagcaacatc tgtcactctc cgttccgcaa atttatgttg
      481 gcagaatggc tcgcggctat gtttctcctg atctgtggga agaaggaata aatgctgggc
      541 ttctaaacta tagttttaat ggtaattcta ttaataatcg tagtaaccat aatgcaggaa
      601 aatccaacta tgcatatttg aatttacaga gtggcatcaa cattggtagt tggcgactac
      661 gcgataactc aacgtggagt tataacagtg ggagtagcaa ttcatctgac agcaataaat
      721 ggcagcatat caatacgtcg gctgaacgtg acattattcc cttacgctca cggttaacgg
      781 taggtgatag ttataccgat ggcgatattt ttgatagtgt gaactttcgt ggcctcaaaa
      841 taaattcaac agaagcgatg ttgcccgata gccaacatgg tttcgctccg gtgattcatg
      901 gtattgcccg tggcaccgca caagtgagtg taaaacagaa cggatacgat gtttatcaga
      961 ctactgttcc acccggccct tttactattg atgatatcaa ctctgcggcc aatggtggtg
     1021 atctgcaagt aaccataaaa gaggcagacg gcagtattca gacattatat gttccttatt
     1081 cgtctgttcc ggttctccaa cgtgctggat atacgcgtta tgcgcttgcc atgggggaat
     1141 atcgtagtgg aaataacctg caaagctccc ccaggttcat acaaggtagc ttgatgcatg
     1201 gactggaagg aaactggaca ccttatggcg gaatgcaaat tgcagaagat tatcaggcct
     1261 tcaaccttgg tattggtaaa gatttaggac tttttggtgc cttttctttc gatatcacgc
     1321 aggccaatac gacacttgca gatggcaccc gtcacagcgg gcaatcggtt aaatccgtct
     1381 acagcaaatc cttctaccag acaggaacca atatccaggt cgcaggatat cgctattcta
     1441 cgcaaggttt ttataactta tccgacagtg cctacagtcg aatgagtggt tacaccgtca
     1501 agcctcctac cggagacagc aatgagcaga cacaatttat tgattatttt aatctgttct
     1561 acagtaagcg tggtcaggaa caaataagca tctctcagca gcttggaaat tacggtgcga
     1621 catttttcag tgccagtcgc caaagttact ggaacacgtc acgcagcgac cagcaaatat
     1681 catttggatt aaatgtgccg tttggtgata ttacgacttc gctgaattac agctattcca
     1741 ataatatatg gcaaaacgat cgggatcatt tactcgcttt tacgcttaat gttcccttca
     1801 gtcattggat gcgtacagac agtcagtcgg catttcgtaa ttcaaacgcc agttacagta
     1861 tgtcaaacga tttgaaaggc ggcatgacca atctatcggg ggtttatggc actctgctgc
     1921 cggataataa cctgaattat agcgttcagg tcggtaacac ccacggaggt aatacatcgt
     1981 ctggcaccag tggttacagt actcttaatt atcgtggagc ttacggcaat actaatgtcg
     2041 gttacagtcg gagtggtgac agcagccaga tttattacgg aatgagtggt gggattattg
     2101 ctcatgctga tggcatcacc tttggacagc cgctgggcga cacaatggtt ctggttaagg
     2161 ctcctggcgc tgataatgtc aaaatagaga accagaccgg aattcatacc gactggcgtg
     2221 gctatgccat attaccattt gcgacagaat atagagaaaa tcgtgtcgct cttaacgcga
     2281 attcccttgc agataatgtt gaactggatg aaaccgtggt cactgtcatc ccaactcacg
     2341 gtgctattgc cagagcaaca tttaatgcac aaatcggcgg gaaagtatta atgacgttga
     2401 agtacggtaa taaaagcgtt ccattcggtg caattgtcac tcacggagag aataaaaatg
     2461 gcagcattgt cgcggaaaac ggtcaggttt atctgactgg acttccacag tcagggaaat
     2521 tacaggtttc atggggcaat gataaaaact caaactgtat tgtcgattac aagcttcctg
     2581 aagtctctcc tggaaccttg ctgaaccagc agacagcaat ctgtcgctaa ggaacaatag
     2641 attatgaaat acaataacat tattttcctc ggtttatgtc tggggttaac cacctattct
     2701 gctttatccg cagatagcgt tattaaaatt agcgggcgcg tcctcgatta tggctgcaca
     2761 gtctcatcgg attcgcttaa ttttaccgta gatctccaaa aaaacagtgc cagacaattt
     2821 ccaacgaccg gtagcacaag tccagccgtc ccttttcaga ttacgttaag tgaatgcagc
     2881 aaagggacaa cgggggttcg ggttgcattt aacggtattg aggacgcaga aaataatact
     2941 ctgttgaaac tggatgaggg aagcaatacg gcctccggtt taggtataga aatactggac
     3001 ggaaatatgc gtccggtgaa actgaatgac cttcatgccg ggatgcagtg gatcccactg
     3061 gtaccagaac agaacaatat tttgccttac tccgctcgtc tgaagtcaac tcagaagtcc
     3121 gtcaatccgg gactggtgag ggcttcggca acctttaccc ttgaatttca ataatcagag
     3181 tcagatatga aaagacttca caagaggttc ctcttagcta cgttttgcgc gttattaaca
     3241 gcaactctcc aggccgccga tgtcactatc actgttaatg gtcgggtagt cgctaaaccc
     3301 tgcactattc aaaccaaaga agctaacgtt aatctcgggg atctttatac gcgcaatctg
     3361 caacaacctg gttctgcatc tggctggcac aatattactt tgtcattaac cgattgtccg
     3421 gctgaaacaa gtgcagtgac ggcaatcgtg acaggttcaa ctgacaatac gggttattac
     3481 aaaaatgaag gtactgccga aaatattcag atagagctta gggatgacca ggatgcgacg
     3541 ttaaaaaatg gcgatagcaa aacggttatt gttgatgaga tcactcgtaa tgcacagttt
     3601 ccacttaagg caagagctat cacggtgaat ggaaacgcaa gccagggaac gatcgaggcg
     3661 ctaatcaatg tgatctacac ctggcaataa cgtcgattcg attttcttag ccttcttttt
     3721 aaatcaattt tttaacctta ggatatatca tgggtaaaac aatcagtata aaggtcctgt
     3781 tcggtattta tcttctatta atggcaggca aagtttttgc cttcagttgt aatgtcgatg
     3841 gcgggtcaag tattggagct ggtacaacct ctgtttatgt taatctcgac cctgtaatac
     3901 agccgggcca gaatctggtt gtagacttgt ctcagcatat aagttgctgg aatgattacg
     3961 gcggctggta cgacactgat catataaacc tggtacaagg ctcagctttc gcaggctctc
     4021 tacagtcata taaaggatcg ctgtactgga ataatgtgac ctaccccttc ccgttgacga
     4081 caaataccaa cgtattggat attggtgata agacaccgat gccattaccg ttaaaactct
     4141 atattacccc agtcggggcg gccggtggag ttgtcataaa agccggggaa gttattgccc
     4201 gtattcatat gtataaaatt gccacgttag gaagcggaaa tccgcgtaat tttacgtgga
     4261 atattatttc aaataacagt gttgttatgc caacaggcgg ctgcactgtt gatagtcgta
     4321 atgtcaccgt gaatctcccg gactttcctg gaagcgcaga aatccccctt ggcgtatatt
     4381 gctcaagcga acaaaagctc tcattttatc tttcgggtac taccactgac agtgctagac
     4441 aagtgtttgc taacacagca ccagatgcaa caaaagcatc gggagtgggt gtctctctga
     4501 tgcgtaatgg aaaaatactg gctacgggag aaaatgtctc cctggggaca gtaaataaaa
     4561 gcaaagtacc tctgggttta agcgcaacct atggacaaac agggaacaaa gtttcagccg
     4621 g
//
LOCUS       DBFIFM010000275         4615 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.275, whole genome shotgun sequence.
ACCESSION   DBFIFM010000275 DBFIFM010000000
VERSION     DBFIFM010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.275"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..607)
                     /locus_tag="N7C74_004437"
     CDS             complement(<1..607)
                     /locus_tag="N7C74_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGU2434167.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPE"
     gene            complement(722..797)
                     /locus_tag="N7C74_004438"
     tRNA            complement(722..797)
                     /locus_tag="N7C74_004438"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(762..764),aa:Thr,seq:ggt)
     gene            complement(804..878)
                     /locus_tag="N7C74_004439"
     tRNA            complement(804..878)
                     /locus_tag="N7C74_004439"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(843..845),aa:Gly,seq:tcc)
     gene            complement(995..1079)
                     /locus_tag="N7C74_004440"
     tRNA            complement(995..1079)
                     /locus_tag="N7C74_004440"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1043..1045),aa:Tyr,seq:gta)
     gene            complement(1088..1163)
                     /locus_tag="N7C74_004441"
     tRNA            complement(1088..1163)
                     /locus_tag="N7C74_004441"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1128..1130),aa:Thr,seq:tgt)
     gene            1525..2475
                     /gene="coaA"
                     /locus_tag="N7C74_004442"
     CDS             1525..2475
                     /gene="coaA"
                     /locus_tag="N7C74_004442"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HGU2434168.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2504..3469)
                     /gene="birA"
                     /locus_tag="N7C74_004443"
     CDS             complement(2504..3469)
                     /gene="birA"
                     /locus_tag="N7C74_004443"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HGU2434169.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3466..4494)
                     /gene="murB"
                     /locus_tag="N7C74_004444"
     CDS             complement(3466..4494)
                     /gene="murB"
                     /locus_tag="N7C74_004444"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HGU2434170.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGVAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1168 a         1163 c         1054 g         1230 t
ORIGIN      
        1 gttccggaat gtaggaatcc aagaagccag ccagttccag gattttcgct tcccactctg
       61 cgtcgccttc cagcgctttc agagcagaac cacgaacgat cggagtgtcg tcgcccggga
      121 agtcgtactg agacagaagt tcacgaactt ccatttcaac cagttccagc agctcttcgt
      181 catcaaccat gtcgcatttg ttcaggaaca cgatgatgta cggaacgcct acctgacgac
      241 ccagcaggat gtgctcacga gtctgcggca tcgggccgtc agtcgcagca actaccagga
      301 tcgcgccgtc catctgagca gcaccggtga tcatgttttt aacatagtcg gcgtgccccg
      361 ggcagtctac gtgtgcgtag tgacgggtcg gggtgtcgta ttcaacgtga gaagtgttga
      421 tggtgatacc acgagctttt tcttccggcg cgttatcgat ctggtcgaat gcgcgagcag
      481 caccgccgta ggttttagcc agtacggtag tgattgcagc ggtcagcgtt gttttaccat
      541 ggtcaacgtg gccgatagta ccgacgttaa cgtgcggttt tgtacgttca aacttttctt
      601 tagacatcga ttgtccctct aagacacgga taaatcggtg atatcaccac atcaaccagg
      661 caacatgccc gacttgttga atgcaataaa cagaaggaaa aaacagggag gagaaaagaa
      721 gtggtgctga taggcagatt cgaactgccg acctcaccct taccaagggt gcgctctacc
      781 aactgagcta tatcagcaca tcttggagcg ggcagcggga atcgaacccg catcatcagc
      841 ttggaaggct gaggtaatag ccattatacg atgcccgcat cctggaactc ggctacctga
      901 ttttcattct gcactgaata tcgagagaag ctctctttat tcgagccggt aagcgaactt
      961 atcgtctcgg gctacgccat cgcgtggccg aaattggtgg tgggggaagg attcgaacct
     1021 tcgaagtctg tgacggcaga tttacagtct gctccctttg gccgctcggg aaccccaccg
     1081 gacttgatgg tgccgactac cggaatcgaa ctggtgacct actgattaca agtcagttgc
     1141 tctacctact gagctaagtc ggcatcaagt agcgcgcatt ctatggagac atgcgagttc
     1201 atgcaactaa aaaattgcat aatttgtttt attggtcaca ttttatgcga cacgatgaag
     1261 aaacagccga atggagggca gattagcaca ctttttcaac atcattgtgc tcaacaatgc
     1321 gctcctgcta aaccataatt ctttttatca gatggaatat ctgtcacatt gcttttcaac
     1381 gatagcttcc tgggagagat tttttcttat tattcctccc catctggtgt taccctcctg
     1441 cccattaacc cattcaacag aactgtgacg cgccatggca aatatcgctt tgccgataga
     1501 gctatgaccg ccagaaacat gcttatgagt ataaaagagc aaacgttaat gacgccttac
     1561 ctacagtttg accgcaacca gtgggcagct ctgcgtgatt ccgtacctat gacgttatcg
     1621 gaagatgaga tcgcccgtct caaaggtatt aatgaagatc tctcgttaga agaagttgcc
     1681 gagatctatt tacctctgtc acgtttgctg aacttctata taagctcgaa tctgcgccgt
     1741 caggcagttc tggaacagtt tcttggtacc aacgggcaac gcattcctta cattatcagt
     1801 attgctggta gtgtcgcggt ggggaaaagt acaaccgccc gtgtattgca ggcactatta
     1861 agccgttggc cggaacatcg tcgtgttgaa ctgatcacta cagatggctt ccttcaccct
     1921 aatcaggttc tgaaagaacg tggtctgatg aagaagaaag gcttcccgga atcgtatgat
     1981 atgcatcgcc tggtgaagtt tgtttccgat ctcaagtccg gcgtgccaaa cgttacagcc
     2041 cctgtttact cgcatcttat ttatgatgtg atcccggatg gagataaaac tgttgttcag
     2101 cctgatattt taattcttga agggttaaat gtcttacaga gcgggatgga ttatccacac
     2161 gatccacatc atgtatttgt ttctgatttt gtcgattttt cgatatatgt tgatgcaccg
     2221 gaagacttac ttcagacatg gtatatcaac cgttttctga aattccgcga aggggctttt
     2281 acagacccgg attcctattt tcataactac gcgaaattaa ctaaagaaga agctattaag
     2341 actgccatga cgttgtggaa agagatcaac tggctgaact taaagcaaaa tattctacct
     2401 actcgcgagc gcgccagttt aatcctgacg aaaagtgcta atcatgcggt agaagaggtc
     2461 agactacgca aataatttgc aggggagcta atgctcccct ttcttatttt tctgcactac
     2521 gcagggatat ttcaccgccc atccagggtt ttattattcc atcctgctca agcaataaag
     2581 cgccttgttt gtctattccg cgtgaaatgc caaatatttc tttatcacca atgataagtt
     2641 tcactgggcg attaataaaa ttatccagct tttcccagcg cgaaagataa ggtgccaatc
     2701 cttcttgttc gaagagttcc agcgccgcgc gtaattcacg tattagcatg gccgccaacg
     2761 tattacgatc gagattgatc cccgcttcct gcagcgtgat ccacccctga ttaacgacac
     2821 tctcttcaac acgacgcatt gccatgttga tcccggctcc aatgactatt tgcgccgcat
     2881 cgccggtttt gccagtcagc tccacaagaa tgcctgccag cttgcgatcc tgcagataga
     2941 ggtcattagg ccatttgaca cgaactttat ctgctcccag cttgcgtaat acttccgcca
     3001 tcacgatacc gataaccaga cttaaaccaa tcgccgccgc cgggccttgt tccagacgcc
     3061 agaacatcga caaatataag tttgcgccaa aaggcgaaaa ccatttccga ccccggcgac
     3121 cacggccagc ctgctggtat tctgcaacgc aggcatcgcc cgatttaagc tctccgatac
     3181 gatcaagaag gtactgattc gtggagtcaa tcactggcag cacagttaca ctaccgccat
     3241 ccagctgacc caatatctgt tcagcattaa gtaactggat aggctcaggc aggctgtatc
     3301 ctttacccgg aacggtaaag acatcaacgc cccagtcacg cagtgtctga atgtgtttat
     3361 taatagccgc ccggctcatt cctaacgttt cacccaactg ctcacctgag tgaaattcgc
     3421 cattcgctaa cagcgcgatt aatttcagtg gcacggtgtt atccttcatg aaattgtctc
     3481 cactgcgctc acttcacctg atgcaccaat aaagcggact tcaggctcaa gccagacatt
     3541 aaatttttca cccacttttt gtcttacatg gtgtgccagt tgcaccacat cttcgctttt
     3601 tgcattgtct tcattaatga gaactaacgc ctgttgacgg tgcaccgcaa ccccacccat
     3661 ttgcatccct tttagctggc actgatcgat aagccaacct gctgccagtt ttactgaacc
     3721 atccgcctgg gggtaatttg gtgctgttgg aaattgtgac agtaatgctt tagccgtttc
     3781 ggcagataca acagggtttt tgaagaaact accggcattg ccattcactt ttggatcagg
     3841 gagtttggtg gtgcgcatat gacacaccgc atcaaatact tgctgtggcg ttactgttgt
     3901 aggatccaga cgagttaagt caccatacgt tagtacaggt tgccactctt ttggcagacg
     3961 cagacctacg gctacaatgg cgaagcggtc ctggtattca tgtttaaaaa tactgtcgcg
     4021 atagccaaaa cggcactctt tggcagttaa gcgcacttgc ttgcctgtcg ccagttcaac
     4081 acaatcaaca taagcgcaaa ctcgctgtaa ttctacgcca taagcaccaa tattctggat
     4141 aggtgatgag ccgacacaac caggaattaa tgccagattt tccagaccag gcataccttc
     4201 ctgcaaagtg tattttacca ggcgatgcca gttttctccg gctcctacat gtaaatacca
     4261 cgcatcaggt tcatcatgaa tttcgatacc tttgatccgg ttgatgatca ccgtgccgcg
     4321 atagtcttcc agaaaaagta cattacttcc ttcacccaga ataagaacgg gttgcccttc
     4381 tgcggttgca tgctgccagg cattgagtag ttgttgttcg tcttcggcac atacaatgtg
     4441 ctgagcatta tgatcaatgc caaatgtgtt ccagggtttt aaggagtggt tcatagctgc
     4501 tttcctgatg caaaaacgag gctagtttac cgtatctgtg gggggatggc ttgtagttat
     4561 gacgacaggg agagtttgta gaaacgcaaa aaggcccagt cttccgaccg ggcct
//
LOCUS       DBFIFM010000276         4601 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.276, whole genome shotgun sequence.
ACCESSION   DBFIFM010000276 DBFIFM010000000
VERSION     DBFIFM010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.276"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            387..986
                     /locus_tag="N7C74_004445"
     CDS             387..986
                     /locus_tag="N7C74_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU2434171.1"
                     /translation="MTSLSPDTVRRIEDAAAALIAAGTPNPTNEQVRQHLGGGSLSHI
                     SPVMRAFRARQREQATPLPPELAQLLTGQLGLLWQAAVKQAEAGALAAREQADDDIAR
                     ADKERDEALANVAALESELAVLREVVAERDRLLQEVRELRAEALPLREQVARLTATGE
                     HLAAQLQDTKAELKEAREDGRQLQTELLALARQDGKVKK"
     gene            983..1603
                     /locus_tag="N7C74_004446"
     CDS             983..1603
                     /locus_tag="N7C74_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434172.1"
                     /translation="MRQERVLRIKAFRRALELGGGERADGCRHFPRWKTELRNFPHSS
                     CDLASNTLAYYLKERERCHPCIIFMEGNAGFHEAENSTVHGHVIVLLDGEYIDLTLDQ
                     FDEYPDYIPYEPVESDGQIGKLLRNIMKHEDPVKTRQIDLDGGEELYAWLRDTADEVL
                     AADPEWQARERSIEEAREAAITVFPFLSDVQKTECEQGPDSQEAAR"
     gene            1600..1800
                     /locus_tag="N7C74_004447"
     CDS             1600..1800
                     /locus_tag="N7C74_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434173.1"
                     /translation="MKIYHYTDLNGLKGIIESGSLWATHFSFLNDSNELTHGMNCLEN
                     ALQYLRDDFNPKTLKFIEQAPL"
     gene            2208..3185
                     /locus_tag="N7C74_004448"
     CDS             2208..3185
                     /locus_tag="N7C74_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HGU2434174.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PVSLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(3470..4210)
                     /locus_tag="N7C74_004449"
     CDS             complement(3470..4210)
                     /locus_tag="N7C74_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2434175.1"
                     /translation="MNNVIPLQNSPERVFLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGARPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(4331..4561)
                     /locus_tag="N7C74_004450"
     CDS             complement(4331..4561)
                     /locus_tag="N7C74_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HGU2434176.1"
                     /translation="MWILNVFQVHKSVRIYIAHFSVFNCHLHPSIDRSLASWHYPNSP
                     KNGKPALSPRHDWLNTGSLLQNVPLRIWFYPQ"
BASE COUNT         1127 a         1204 c         1251 g         1019 t
ORIGIN      
        1 cttataaaaa caaagcgtaa ttaccgcatt cccgtttcgt atggatccga ctttatttga
       61 ataaacgact ttccggaagt gtaccacctg agaaaatcat catatcgctg ttttccgccc
      121 ctgcgcggcg catcacagtc cagagataca acacaacata tagtgccatg ggcaactgaa
      181 tgaggcactg catgctgtgc tgacgggatg taacgctgtg cggagggaga ggaagatccg
      241 taaccaccag aaaccggatc tgaccaggaa aagagaaaga gtgtatgtaa gttctgtact
      301 gtatgtatga attgtattgt atgtattgtg tatttttcat accgggcata cgatggcgcc
      361 attccctgag aaaccggagc cagaccatga catccctctc cccggacacc gtccgccgca
      421 ttgaagacgc cgccgccgcc ctgatcgccg ccggcacccc gaacccgacc aacgagcagg
      481 tccgtcagca cctcggcggc ggctccctgt cccacatctc cccggtgatg cgcgcgttcc
      541 gcgcccgcca gcgcgagcag gccacgcccc tgccgccgga gctggcgcag ctgctgaccg
      601 gccagctggg gctgctgtgg caggcggccg tgaaacaggc tgaagccggg gccctggcgg
      661 cgcgcgagca ggccgacgac gacattgccc gggccgacaa ggagcgggat gaggcgctgg
      721 cgaacgtggc cgcgctggaa agcgagctgg cggtgctgcg cgaggtggtg gccgaacgtg
      781 accgcctgct gcaggaggta cgcgagctac gcgcggaggc gctgccgctg cgcgagcagg
      841 tggcccgcct gaccgccacc ggcgaacacc tggcggcgca gctgcaggac acgaaggcgg
      901 agctgaagga agcccgggag gacggccgcc agcttcagac cgaactgctg gctctggccc
      961 gccaggacgg gaaggtgaag aaatgagaca ggaaagagta ttacgtataa aggcatttcg
     1021 acgggcactg gagcttggtg gcggtgaacg ggctgacggg tgtcgtcatt ttccccgctg
     1081 gaaaacggaa ctgaggaatt tcccgcacag cagctgcgat ttagccagca atactctggc
     1141 gtactacctc aaagaacgag agagatgcca tccgtgcatc atttttatgg aagggaacgc
     1201 tggctttcat gaagcggaaa actcaacagt tcacgggcat gtcatcgtat tgctggacgg
     1261 agagtacatc gatttgacgc tggatcaatt cgacgaatat ccggactaca ttccttacga
     1321 acctgtcgaa tctgacgggc agattggtaa gctgttgcgt aatatcatga agcatgagga
     1381 cccggtaaaa acccgccaga ttgatctcga tggtggcgaa gagctgtatg cctggttacg
     1441 cgataccgcc gacgaagtgc tggcagcaga tccggaatgg caggcccggg aacggtcgat
     1501 agaagaagca agggaagccg ctattacggt gttcccgttt ctgtctgacg tgcaaaaaac
     1561 tgagtgcgaa cagggcccgg acagtcagga ggctgccaga tgaagattta tcattacact
     1621 gacctgaacg gcctaaaggg catcatcgaa tcaggctcac tgtgggcgac gcacttttcc
     1681 tttctcaatg acagtaatga gctgactcac ggcatgaact gccttgagaa tgccctgcag
     1741 tacctgaggg acgactttaa cccgaaaacc cttaagttca tcgaacaggc acccttataa
     1801 gcggttgtgt atgctgtttc cctggaatga tttatccaca tatccggagg ctcgatccag
     1861 taaacagatc catgaatgat caacaaagga tccattaaag atccccatac cgctgcaaac
     1921 cttgtcactc atgggccggg accacgatca cataagcagt ggcatgttac tgataaactg
     1981 taacatgcta atgataagct gtattcagta atccatatac tgaagtaagt taatgacata
     2041 aactatggtc agtacgccag actcagctgt taaatacagg ctgcaggttt ttcttcagtc
     2101 agttagcggg gctctgacac acgatttgct gtttattctt ttactgtcca caggcaggag
     2161 gctttctgga aaacgaaaat tcagacatca aaaaactgtt cggcgaggtg gataagtcgt
     2221 ccggtgagct ggtgacactg acaccaaaca ataacaacac cgtacaacct gtggcgctga
     2281 tgcgtctggg cgtttttgta ccgaccctta aatcactgaa gaacagtaaa aaaaatacac
     2341 tgtcacgtac tgatgccacg gaagagctga cacgtctttc cctggcccgt gctgagggat
     2401 tcgataaggt tgagatcacc ggcccccgcc tggatatgga taacgatttc aagacctggg
     2461 tggggatcat tcattccttt gcccgccata acgtaattgg tgacaaagtt gaactgcctt
     2521 ttgtcgagtt tgcaaaactg tgtggtatac cttcaagcca gtcatcacgc aggctgcgtg
     2581 agcgcatcag cccttccctg aaacgcattg ccggtaccgt gatctccttt tcccgcaccg
     2641 atgagaagca cacccgggaa tacatcaccc atctggtaca gtcagcctac tacgatactg
     2701 aacgggatat tgttcagtta caggctgatc cccgcctttt tgaactgtac cagtttgaca
     2761 gaaaggtcct tctccagctt aaggcgatta atgccctgaa gcgacgggag tccgcccagg
     2821 cactctacac ctttatagag agcctgcccc gggatccggc accggtatcg ctggcgcggc
     2881 tgcgtgcacg cctcaatctg aagtctcctg tattttccca gaaccagacg gtcagacggg
     2941 caatggagca gctgcgcgag attggatatc ttgattacac ggagatccag cgggggcgga
     3001 caaaactctt ctgcattcac taccggcgtc cccggttaaa agcaccgaat gatgagagta
     3061 aggaaaatcc gttgccacct tcacctgcgg aaaaagtcag tccggagatg gcggagaagc
     3121 ttgccctgct tgagaaactg ggcatcacgc tggatgacct ggaaaaactc ttcaaatccc
     3181 gctgaacata aactgtagtc agtgaagagt gttcctttac tgactacagc ttatattatc
     3241 aggtgcagtg agtggtctgc tcactgcagt ttatattcag tttcctgcag tgctgcctgt
     3301 agctgagctg tcatctgccg gtcccttacg tgagtcaccc cgtaacctga tgctgaggca
     3361 ttgctccctt cataaaacat gacttactca ctacagctta tatacatgct ccagcttatg
     3421 ttatgtctgt tctgctgacc acagcttata taaggaagcg cgtatgccgt cacctcaggg
     3481 gaggcagtgt acgcaggatc tccgcagcat cccgcccgtc acctgtgaaa ggcactgcca
     3541 gcgtggcagc catatccagc gcaaacactc tggtataaac ctccatcgaa cgtggatccc
     3601 tgtgacctgc cagggcctgg atgactttcc ggggctggcg gtgatagagc atatgcatga
     3661 tatagctgtg ccggaaggtg tgtggtgtga ccggaatcga aaagtgtact ccgtcagctt
     3721 cggcccgtct gacagcctgc ttcagccagt tgcgcatggt ttcgtcggtc acggcccata
     3781 atggttcacg acgacggggc cgggtggtga tcatccagct ttccatctgc ctgacatagc
     3841 ttatatccgt cagcggaacc aggcggactt catcttttgg cgggcgtccg cgtcgcgcac
     3901 gcactttttc ggacaggatc cgcacaaacg gccttgctcc atccaggtca aatgattccg
     3961 gcgtcagcat ccgggcttcg ccaatacgca ttccggtatt ccagagggtg gcgaacagca
     4021 tatggtgacg ctgatccggc atatagaaaa gaagggcact cacttccggg gccagcaggt
     4081 aggccggtgt ggcccctgtg gaagtggcca ttcttctcag ggagagcgct gttgcaaaat
     4141 ccacccccgg cgcaatgggt aacaggaaga cgcgttctgg tgaattctgc aggggaatga
     4201 cattgttcat gagttatttt tcacgtagca cacgcactgt ttatggatac aggtattctg
     4261 acctgaacgc ccggatcctt ccacgacaat aaatcgcagc gtgcggatcc ttttgtttgc
     4321 ttatcacgca ttattgcggg taaaaccaaa tacgtaaagg tacgttttgc agcagggatc
     4381 ccgtattaag ccagtcgtgg cgcgggctga gggctggttt gccgtttttg ggagaattgg
     4441 ggtaatgcca ggacgctaat gatctatcaa tggatggatg taagtgacaa ttgaaaactg
     4501 aaaaatgcgc tatgtagatt ctgacagatt tatgcacctg aaatacgttc aaaatccaca
     4561 tagcgtaact ctgattttcc ttaccagaaa aaagaggggg a
//
LOCUS       DBFIFM010000277         4587 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.277, whole genome shotgun sequence.
ACCESSION   DBFIFM010000277 DBFIFM010000000
VERSION     DBFIFM010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.277"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            160..2592
                     /gene="hsdR"
                     /locus_tag="N7C74_004451"
     CDS             160..2592
                     /gene="hsdR"
                     /locus_tag="N7C74_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HGU2434177.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVKDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2659..4128
                     /locus_tag="N7C74_004452"
     CDS             2659..4128
                     /locus_tag="N7C74_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGU2434178.1"
                     /translation="MSISSVIKSLQDIMRQDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSRDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPAELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4128..>4587
                     /locus_tag="N7C74_004453"
     CDS             4128..>4587
                     /locus_tag="N7C74_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGU2434179.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVRISEIGHDWGQKTPDKDFTYIDVGSINKEYGIIEELSILSAKDAP"
BASE COUNT         1332 a         1124 c         1124 g         1007 t
ORIGIN      
        1 tctgtctctg atatgcccag attaattcaa gcaagatata acagagatat caatgtgatc
       61 tctgctacgt actttacgtt gaaacaccgc cctaaatcat agccaggcta ttgattttat
      121 gtacaatcag aatcgtcctg ataaaagcga gaatacccca tggcggaact gaacctaagt
      181 aacctgacgg aagcagacat cattaccaaa tgcgttatgc cagccattct caatgcaggc
      241 tgggacaaca caacgcaaat cagacaggag gtcaaactcc gggacggtaa agtcatcgta
      301 cgtggtaaag ttgcggcacg cagaacggta aaatctgctg acatcgtgct gtatcacaaa
      361 cctggcattc ccttagccgt gattgaagca aaagccaaca aacatgaaat tggcaaaggg
      421 atgcaacagg gcattgaata tgcgcgcctg ctggacgttc cttttgtttt tgccaccaac
      481 ggtgatggct ttatcttccg cgatgccacc gcagccgaag gtgaatgcct tgaaaagcaa
      541 ataacgctgg atgacttccc ctcccccgct gaactctggc aaaaattctg cctctggaaa
      601 ggatacacac aagctcagct tccggtgatt actcaggact attacgacga tggcagcggt
      661 aaatcgccac gttattacca gcttcaggca atcaacaaaa ccattgaagc cgtctccaac
      721 gggcaaaacc gcgttctgct ggtcatggcg accggaacag ggaaaaccta taccgcattc
      781 cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac gcattttgtt ccttgccgat
      841 cgcaatattc tggtcgacca aaccaaaaat aatgatttcc agccatttgg tacggcaatg
      901 accaaagtca gcggacgcac cattgatccc gcttatgaaa ttcacctcgc gctctatcag
      961 gctataactg gcccggagga agaccaaaaa gcgtttaaac aagtcgcacc agatttcttc
     1021 gatctgatcg tgatcgacga atgccatcgc ggcagcgcat ccgaagacag cgcctggcga
     1081 gaaatccttg attattttag ttccgccacc caaattggct taaccgccac gccaaaagag
     1141 acgcatgaag tctccagcac ggattacttc ggcgatccgg tttacgtcta ctcactaaaa
     1201 gaagggatcg aagacggctt cctcgcccct tataaagttg tccgtgttga tattgatgtt
     1261 gatctgcaag gctggcgccc aaccaaaggg caaactgact taaacggcga agtgatcgac
     1321 gatcgtatct ataaccagaa agatttcgat cgcacgatgg taatcgacga acgcacagaa
     1381 ctggttgcca gaaccattac cgactacctc aagcgtacca atccgatgga taaaaccatc
     1441 gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc gcgccctggt taatctcaac
     1501 ccggagcagg tgaaaaagaa cgacaaatac gtcatgaaaa tcaccggcga tgatgaaatt
     1561 ggcaaagctc agttggataa cttcatcaac ccgaaaaaac cgtacccggt tatcgcgacc
     1621 acttcagagc tgatgaccac cggtgtggat gctaaaacct gcaaactggt agtactggac
     1681 cagaacatcc agtcgatgac caaattcaag cagattatcg gtcgtggtac acgcatcgac
     1741 gaacgttacg gcaaactctg gtttaccatc ctcgacttta aaaaagccac cgaactgttt
     1801 gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg ataccacacc agaggatatc
     1861 gccgatccag aatctgattt tgaagagaaa ctcgaagaaa tcagcgaaca tgacgacgaa
     1921 caggtaacag gcgttgatga accgcctgcg ccaccatacc aggttaaaga taccgatgat
     1981 gtcggcccac ttccggaaga agacgagaag aaaatccgca agtttcacgt caacggtgta
     2041 gcagtgggcg ttattgccca gcgtgttcag tattacgacg ccgacggtaa actggttacc
     2101 gaatccttta aagattacac ccgcaaaaca ctgctcaaag aatatgcctc gctggatgac
     2161 tttacccgca agtggcagga cgccgatcgc aaagaagcga tcattcacga gctggagcaa
     2221 caggggatca tctgggaagt actggcagaa gaagtcggta aagatctcga cccgttcgac
     2281 atgctttgcc acgtagtgta tggtcagccg ccgttaaccc gcaaagagcg cgccgagaac
     2341 gtgcgcaagc ggaactactt cacaaaatac tctgaagcag cgcaagccgt gctcgataat
     2401 ctgctggata aatacgccga tgcgggcgta caggagatcg aaagtattca ggtgctgaaa
     2461 cttaagccat tcgacagcat gggcacctta ccggagatta ttaaaaccgg atttggcgac
     2521 cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg aaatctacca attaccgccc
     2581 cgctctgctt aacaactctc ttttgcgcct tctgcaacca tcatgttgca gaaggccttg
     2641 tttttcatgg aatacaacat gtctatcagc tcagtaatca aatcattaca agatattatg
     2701 cgccaagatg ccggtgtgga tggcgatgcg cagcgtctcg gtcagctctc ctggctgctg
     2761 tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac tggagcagga taactatcaa
     2821 tatccgatcc cacagcgtta tttatggcgc agttgggccg ccaacgcgca gggcattacc
     2881 ggtgattccc tgctggaatt cgttaatgat gatctgttcc cggcgttgaa aaacctcact
     2941 gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc aggcgttcag cgatgcctat
     3001 aactatatga aaaacggtac gctactgcgt caggtgatca ataagctgaa cgaaattgac
     3061 tttaccagcg ccagcgaacg ccatctgttt ggtgatattt acgaacagat ccttaaagat
     3121 ctgcaatctg cgggcaatgc gggcgaattc tatactccac gcgccgtcac tcgctttatg
     3181 gtggatcgcg ttgatccgaa actcggcgaa tccattatgg acccggcctg cggtacgggc
     3241 ggttttcttg cctgcgcatt tgatcatgta aagaacaaat acgtgaagag cgtcgccgat
     3301 catcagacgc tgcaacaaca gatccacggt gttgagaaaa aacagcttcc gcacctgctg
     3361 gcgaccacca atatgctgct acacggcatt gaagtgccag tgcaaattcg tcacgacaac
     3421 actctgaaca aaccgctttc ctcccgggat gagcaactgg atgtcattgt taccaacccg
     3481 ccgtttggtg gcacggaaga agacggtatt gagaagaact ttccggcaga gatgcaaacc
     3541 cgcgaaacgg cggatttgtt cctgcaactg attgtggaag tactggcgaa aaatggtcgc
     3601 gcggcggtgg tattgccgga tggcacacta tttggcgaag gcgttaaaac caaaatcaaa
     3661 aagctactta ccgaagagtg caacctgcat accatcgtgc gcttaccgaa tggtgtgttt
     3721 aacccctata ccggcattaa aaccaacctg ttgttcttta ccaaaggtca gccaaccaaa
     3781 gagatttggt tctatgagca tccgtatccg gcgggcgtaa aaaactacag caaaactaag
     3841 ccgatgaagt ttgaagagtt tcaggcggag atcgactggt ggggtaacga ggccgatggt
     3901 tttgccagcc gcgtagagaa tgagcaggcg tggaaagtca gcattgatga cgtgattgcg
     3961 cgtaacttca atctggatat taaaaaccca catcaggcgg aaaccgtcag ccatgatccg
     4021 gccgagctgt tagcgcagta tgcaaaacag caggcggaga tccagacgct gcgtaatcaa
     4081 ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg ttaactaatg agtgtggaaa
     4141 agctgatcgt tgatcatatg gaaacctgga cctcggcgtt gcaaacccgt tccaccgccg
     4201 ggcgcggcag ttccggaaaa attgatttat atggcattaa gaaattacgt gagctgattc
     4261 tggaactggc tgtgcgcggt aaactggtgc cgcaggaccc gaacgatgaa ccggcgtcgg
     4321 agctgctgaa gcgtattgcg gcagaaaaag cagagctggt gaagcaaggt aaaattaaaa
     4381 agcaaaaacc actgccggaa attagtgagg aagagaagcc gtttgaattg ccggagggat
     4441 gggagtgggt gcgtattagt gaaattggac atgactgggg acaaaaaact ccagataaag
     4501 attttactta cattgatgtt ggttcaataa ataaagaata tggaattatt gaagagctat
     4561 ctattctttc agcgaaagat gccccat
//
LOCUS       DBFIFM010000278         4573 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.278, whole genome shotgun sequence.
ACCESSION   DBFIFM010000278 DBFIFM010000000
VERSION     DBFIFM010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.278"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            4..306
                     /locus_tag="N7C74_004454"
     CDS             4..306
                     /locus_tag="N7C74_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU2434180.1"
                     /translation="MTLKEFIKSLRVGDAKKFAARLGVSPSYLSQMASGRTAISPTRA
                     LMIESATEGQVSRAELRPHDWELIWPEYASGIRLGQTHVVHAEGDCSACLSDGVDS"
     gene            303..728
                     /locus_tag="N7C74_004455"
     CDS             303..728
                     /locus_tag="N7C74_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU2434181.1"
                     /translation="MKIKHEHIRMAMNVWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            751..1713
                     /locus_tag="N7C74_004456"
     CDS             751..1713
                     /locus_tag="N7C74_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2434182.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIAPSTVDPSNFDGSTVDNKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQNFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASDDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            1720..2460
                     /locus_tag="N7C74_004457"
     CDS             1720..2460
                     /locus_tag="N7C74_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU2434183.1"
                     /translation="MKNIAAVGVLERIRRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRLNHQVRVEKILNRAGIQPLHRKCSFGNYRVQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHDGYDNGQSGEKFLQE
                     LCGVDLLVLDEIGMQRDTRNEQVTLNQIVDRRTASMRSVGMLTNLNHAAMSTLLGDRV
                     MDRMTMNGGRWVNFNWESWRSNVGRQGM"
     gene            2486..3255
                     /locus_tag="N7C74_004458"
                     /pseudo
     CDS             2486..3255
                     /locus_tag="N7C74_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            3271..3666
                     /locus_tag="N7C74_004459"
     CDS             3271..3666
                     /locus_tag="N7C74_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HGU2434184.1"
                     /translation="MAKPFTHEQREELKARIIGLVRKNERMTISQLERATGAGWHSVR
                     RCLVDVLACGDLYMPGKYGVFTSEQVYRVWRKAAEKATDQTLIRKLPDGEIRRYDRQQ
                     NIICGECRKSEVMLRVLAFYQGNFQEAVL"
     gene            3723..4307
                     /locus_tag="N7C74_004460"
     CDS             3723..4307
                     /locus_tag="N7C74_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU2434185.1"
                     /translation="MTTFTKEQLISHVSENVKAMKFAVKQTAFKNSLEAIELDLALAL
                     VAQASLEAEPVLYMNRFTGKTFSLEEQPGADKEPEIYVPLYAAPPDSAAMLQAGNFRE
                     KKGSSTNNFREISETSTNYPVTLDDWISCSERMPDDGQHVIILCDGAFVLYAQYRDGE
                     FFDVVRNGDEFFETQSRNVTDWMPLPEPPQEVRQ"
     gene            4423..4527
                     /locus_tag="N7C74_004461"
     CDS             4423..4527
                     /locus_tag="N7C74_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434186.1"
                     /translation="MSDDISLAMEGALAVIAVVGVYCLVVFLMDRLGN"
BASE COUNT         1202 a          981 c         1344 g         1046 t
ORIGIN      
        1 ggtatgacat taaaagagtt tattaaatca ttaagggttg gtgatgctaa gaaattcgcg
       61 gccagacttg gtgtatcgcc atcttactta tcgcaaatgg cgtctggacg aacagctata
      121 tctccaaccc gcgcccttat gatcgaatct gcgacggaag gccaagtaag tagggcggag
      181 ctacgacccc atgattggga gcttatttgg cctgagtatg cgagcggcat tcgtttgggg
      241 caaacacatg tagttcatgc tgaaggtgat tgtagtgcat gcttatctga tggagttgat
      301 tcatgaaaat caagcatgaa cacatccgca tggcgatgaa tgtctgggcg catccggacg
      361 gcgaaaaagt accggctgcg aaaattacca aagcgtattt cgagctggga atgacgttcc
      421 cggaactgta tgacgacagc catccggaag ccctggcccg taatacccag aaaattttcc
      481 gttggctgga taaagacacc cctgatgctg ttgaaaaaat gcaggctctg ttaccggcga
      541 tcgaaaaggc gatgccgcct ttgctggtgg cccgtatgcg cagccacagt tctgaatatt
      601 accgtgagat cgtcgaacgg agggatcggc tggtgaagga tgtcgatgat tttgttgcgt
      661 cagcggttgt tttgtatgac cagatgaatc gcggcggccc ggcagggaat gctgtggtga
      721 tgcactaaaa gcacggtgtt cgggggtttt atgagcagca agcttcatgg tcttgtctgg
      781 gaagggtgcg ccttcaccgg catgatctta tccagggtgg cggttatggc ccgtcttgca
      841 gactacagca atgacgaggg cgtgtcatgg cctgccattg aaactatccg gcgtcagatc
      901 ggtgcaagaa gtgaatccac agtgaaatcg gctattgcag aactggcgaa agagggctgg
      961 ctgacgaagg aagagcgtaa ggtcggtggg cgtaatgtaa gcaatatcta tcggcttaat
     1021 gtggaaaaac tcgaagcagc tgctgcggcg gcgcgtgagt catataaacc gaaaagaaaa
     1081 attagcccgg caaaaaatga cccgttaaca gttgacccgt caaatattgc cccctcaacg
     1141 gttgacccgt caaattttga tggatcaact gttgataaca aactgccgat taggggggcg
     1201 atgattgacc ccgatccgtc agtattaaaa cctgatccgt cagataaaag atcttcttgt
     1261 ccggacgctt cgcaaccgga cccgcagacg gctgaacaga attttttaac ccgacaccct
     1321 gacgcggttg tgttcagtgc gaaaaaacgc cagtggggaa gtcaggaaga tttggtgtgc
     1381 gcacagtgga tctggggacg aatcgtgagt ctttacgagc aggcggccag cgatgatggc
     1441 gagatcacga gaccgaaaga acccaactgg actgcatggg ccaatgacgt gcggacaatg
     1501 cggatgctgg atggcagaac tcacagacaa atttgtgaaa tgtttgggcg tctccagcgg
     1561 gattcgttct gggtaaaaaa catcatgagt ccggcaaaac tccgggaaaa atgggatgaa
     1621 ctggttatcc gcctggggcg ttcgcccgcg cagcgttgcg tgaatcacat ttctgaaccg
     1681 gacactgaaa taccgccggg attcaggggg tgacgtgtca tgaaaaacat tgcggcagtt
     1741 ggggttcttg aacgtattcg cagacttgca ccacaggggg cggttccacc gtaccggacg
     1801 gtggaggagt ggcgggaatg gcaacttgct gaaggacgaa aacgcagcga ggagattaac
     1861 cgcctgaatc atcaggtgcg ggttgaaaaa atcctgaacc gtgcgggcat ccagccgctt
     1921 cacaggaagt gctcattcgg gaactaccgg gtgcagaacg acggtcagcg ccatgctctg
     1981 agccaggcga aatccattgc cgatgaattg atgaccggat gtacaaactt cgtgttcagc
     2041 ggtaaacctg gtaccggtaa aaatcacctg gcagcagcga ttggcaatcg gctgatggcg
     2101 aaggggagaa gcgtgattat cgtcaccgtg tccgatgtca tgagcgtgtt gcatgacggc
     2161 tacgacaacg gccagtccgg ggaaaaattt ttacaggagc tttgtggagt tgaccttctg
     2221 gtccttgatg aaattggcat gcagcgggat acgcgcaacg agcaggtcac gctgaaccag
     2281 atagtcgacc gcagaacggc ttcgatgcgt agtgtcggaa tgctgacgaa cctgaatcac
     2341 gcagcgatga gcacactcct cggagatcgg gtgatggacc gtatgaccat gaatggtggt
     2401 cgttgggtga attttaactg ggagagctgg cggtcaaacg ttggacgtca gggtatgtga
     2461 gaatttttga cgaggtaaat tttcgatgga aactgtattg catgcactga aagcgatggg
     2521 aaaagccaat tctgttgaac tggcggcgcg gcttgatatc agccgtgaag aagttcttaa
     2581 cgaactgtgg gaactcaaaa aaatggcgtt gttgataaaa cgggtcacac ctggtttctg
     2641 gctgtcgaag gtgaagccgg ggtaaccgaa gggcaggcac tacaacctga agcgccggat
     2701 gtggtaaccg aagaggtcgc tccaaaagtt accgcagaca tgatggttga gtttatcggt
     2761 caggatggtg ctaaaacgtg tgaggaactg gcgggtaagt tcggcgtcag tactcgcaag
     2821 gttgcttcca cgctggcggt ggtaaccgca acggggcggc tggcacgcgt taatcagaac
     2881 ggtaaatttc gttactgcat gtcggggggt aatttaccag cagatccgaa agccgcgccg
     2941 gtaacgaaaa atgatggtaa ggcctttcct cagccagcag gtgctgcgtt accagtccgg
     3001 gaagccgcaa cacaggaaga aattaaaaca gaaactgtgg cggacattgt gcagccgttg
     3061 ccatcgttta ccgaaacgca agcagatgag ctgatttttc cgtccctgcg cagggcaaac
     3121 ctggcgctgc gcagggcgaa aagtgatgtt cagaagtggg agcgagtctg cgccgcgctg
     3181 cgggagctga acaagcaccg ggatattgtt cgacagatta ctgattcttc ccgccgtgtt
     3241 gtatcggaaa agtgatagcc ggaggcgctt atggcgaaac cttttacaca cgaacagcgt
     3301 gaagaactga aggcccgaat tatcgggctg gtacgcaaaa atgaacgcat gacgatatca
     3361 caactggaga gagcgacggg agcaggctgg cattcagtca gacgttgcct tgtggatgta
     3421 ctggcttgtg gcgatttata catgcccggt aaatacggtg tttttacatc agaacaggtg
     3481 tatcgcgtat ggcgtaaggc agcggagaaa gcaaccgacc agacattgat tcgaaagtta
     3541 ccagacggag aaatacgccg ctacgacaga caacagaaca taatctgtgg cgagtgccgg
     3601 aagagtgaag ttatgctgcg tgtactggcg ttctatcagg gcaattttca ggaggcggta
     3661 ctgtgagtga attagctatc aggcttcaat tgtcgctggc attcgcatca aaggagaatg
     3721 agatgaccac ttttacaaaa gagcagttaa tcagtcatgt tagtgaaaat gtaaaggcga
     3781 tgaaatttgc agtaaaacag acagcattca aaaattctct cgaggcaatt gagttggatt
     3841 tagcactggc ccttgttgct caggcttcgc tggaagcaga gcccgtgctt tatatgaatc
     3901 gatttaccgg aaagacattc tcactggaag agcaacccgg tgctgataag gaaccggaaa
     3961 tatacgtgcc gctatatgct gccccgccag acagcgccgc catgcttcag gctggaaact
     4021 ttcgggaaaa aaagggttcg tcaaccaata attttcggga aatctcggaa acgtcaacca
     4081 actatccggt aactctggat gattggataa gctgtagtga gcgaatgccg gatgatggtc
     4141 agcacgtaat tattttatgt gatggcgcat tcgttcttta tgcgcaatat cgagacggtg
     4201 agttttttga tgtagtccgt aatggtgatg aatttttcga aacacagagc cgcaatgtaa
     4261 ccgactggat gccgctacca gaaccgccgc aggaggtgcg ccaatgatct ggcctgaagc
     4321 ctttgcaatt acaggcgttg ctatagctat tgatttttta gtatatgtta tttgtcggtg
     4381 ggggtaaaaa cgttcgccgg gattcacacc aaaggaggga atatgtcgga tgatatttca
     4441 ctggcaatgg aaggtgcgct ggctgttatt gctgttgtgg gcgtttactg cctggttgtg
     4501 tttttgatgg atcgactagg gaactgaatt cattacgata tgggaattcc catatcgggt
     4561 aaaaacggtt tgc
//
LOCUS       DBFIFM010000279         4566 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.279, whole genome shotgun sequence.
ACCESSION   DBFIFM010000279 DBFIFM010000000
VERSION     DBFIFM010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.279"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..695
                     /locus_tag="N7C74_004462"
     CDS             <1..695
                     /locus_tag="N7C74_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HGU2434187.1"
                     /translation="IFKRIRNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFI
                     VYPPILSNGTFNFIVMQTLFYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVA
                     YLLNQRYGSGDAWMYETESVITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFI
                     YLVHHPLTLFFGAYITPHITSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKP
                     VVKRENDKAPAR"
     gene            complement(703..2184)
                     /gene="clsC"
                     /locus_tag="N7C74_004463"
     CDS             complement(703..2184)
                     /gene="clsC"
                     /locus_tag="N7C74_004463"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HGU2434188.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMSEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIVLKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2126..2659)
                     /gene="ymdB"
                     /locus_tag="N7C74_004464"
     CDS             complement(2126..2659)
                     /gene="ymdB"
                     /locus_tag="N7C74_004464"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HGU2434189.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(2754..3065)
                     /gene="ymdA"
                     /locus_tag="N7C74_004465"
     CDS             complement(2754..3065)
                     /gene="ymdA"
                     /locus_tag="N7C74_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HGU2434190.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(3186..3518)
                     /gene="csgC"
                     /locus_tag="N7C74_004466"
     CDS             complement(3186..3518)
                     /gene="csgC"
                     /locus_tag="N7C74_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HGU2434191.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(3577..4035)
                     /gene="csgA"
                     /locus_tag="N7C74_004467"
     CDS             complement(3577..4035)
                     /gene="csgA"
                     /locus_tag="N7C74_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HGU2434192.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKDSHMTVKQFGGGNGAAVDQTASNSTVNVTQVGFGNNATAHQY"
     gene            complement(4076..4531)
                     /gene="csgB"
                     /locus_tag="N7C74_004468"
     CDS             complement(4076..4531)
                     /gene="csgB"
                     /locus_tag="N7C74_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HGU2434193.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         1172 a         1135 c         1011 g         1248 t
ORIGIN      
        1 ggatatttaa acgcatcaga aataatttag aaaattctga taaaacgaat aaaaaattct
       61 cgatggtaaa actatcggtg atttttttgt gcctcggcat cggttatgcg gtaataagaa
      121 gaacgatttt tattgtgtat ccacccattc tgagtaatgg cacgttcaat tttattgtca
      181 tgcaaacgct attttattta ccgttcttta tcctcggcgc actggctttc attttccctc
      241 atcttaaagc cttgtttacc acgccgtctc gtggctgtac cctcgctgca gcattggcgt
      301 ttgtcgctta tttactcaac cagcgctatg gcagtggcga tgcctggatg tacgaaaccg
      361 agtcggtgat caccatggtc ctcggtctgt ggatggtgaa tgtggtcttc tcctttggcc
      421 accgtttgct taacttccag tcagcgcggg tgacttattt tgttaacgca tcgctgttta
      481 tctatctggt tcaccacccg ttaacgctgt ttttcggcgc atacattaca ccgcacatca
      541 cctccaactg gcttggtttt ctctgtggcc tgatattcgt agtagggatt gcgataattc
      601 tgtatgaaat tcatttgcgc atcccgttac tgaagttttt gttctctggt aaaccggttg
      661 ttaagcgtga gaacgataaa gcaccagccc gttaagccac atttacaata accattccac
      721 gggcaatatc gacgccagtc tgaccataac ccgcttccag aaactggtag cgggttcttt
      781 tttgagaaca atctctttct ttgcatgacg atcaacccag ttgatccgtc cccacctgtc
      841 cagacggagc tgccaggccg catcatactg gctctgaata aagcgtttat caattaactg
      901 agccagcgtt tcgctctcta tcacgaagcc catttcagta ttgagcaatg ttgaacgcgg
      961 atcgaaattg aaagaaccga taaacaccgt tttaccatcg atgctaaagg ttttagcatg
     1021 caggctggca ccggaattac cggttatgcc gcgatcgtgt aacgtactac tttgttcacg
     1081 cgtcggcttg agttcatata attccacgcc atagcggagc aattttttgc gccagcgcgc
     1141 gtatccggca tggacgacag caacatcgtt agcggcaaga gaattggtta ggatcgcaat
     1201 ctttacccct tttctcacca tccgtaagag ttgcgccaca cctgcgcgtg tcggtacaaa
     1261 ataggaagag ataatatcga tgcgttcact gggtgagccc atgatatcga acaggcgctg
     1321 cggtaacagt gaatgccgtt ttgccttgcc ctcccctttc gccggatcat cacttaataa
     1381 acgtgttttc gcccagataa gcggcaacgt tccatcaacc agatgattta taaacggact
     1441 ggattccatt ttgcgtaagt aacgatgcgt catggcatcg ttatgccagg aggcgggtaa
     1501 ctcgatgcga tccgccattt caccttccgg gacatccagc acctgctgta agggtgaaac
     1561 cgatttgcaa taccagtagc gggcgaaatc atcggcaacg tcctctacta cgggtcctat
     1621 tgccatgaca tctaaatccg aaaaaagtgg ctcttcccct gccccaaaat aggcatcacc
     1681 aatatttcgc ccccctacca acgtcaccac gccatcgaca gtgaaacttt tattgtgcat
     1741 acggcgattg agacgggaaa agtcggtgat ataaccaagc ggacgcagca agcgaaacga
     1801 gaaaggatta aaaagccgga cttcaatgcg gggatggctg tcaagcaagc gtaaaatgtc
     1861 gtcaagtccg ggcgtattgt tgtcgtccag caacaaacgg acgcgaacgc cacgctttgc
     1921 tgcggctaac agggcggaaa acagtaaccg acccgacata tcgtcctgcc agatgtaata
     1981 ctgaacatcc agcgtatgct ctgacatttc ggccagacga taccgggcgg caaacgcatc
     2041 cagacttttc tccagaggga aaagaccaca ctgaccggga tgttgcgaac acagtggcag
     2101 caccgcgctc gccagccggg gcaaatcatt catctccttg ttgggtaagg agtctttcgt
     2161 agaggtgggc gttttcttca tcataacaga caaagtatac ctgttcgggt aaagcgtgac
     2221 gggtaataaa ttctgaaacg gtttttactg cgatttcagc cgctgccgca cgagggtaac
     2281 cataaacccc agtactgatt gcaggaaaag ccactgacgt atagctgttt gccgccacca
     2341 gtcgtaggct attgagatag gcatcctgca aaagctggtc ttcgttttgt tcaccaccac
     2401 gccagactgg cccgacggtg tgcactacgg ctttagcagg aagatcgcct gcaagcgtaa
     2461 taacggcatg ccccgtaggg caatcgccct gctgttgcct gacttttaaa caagcatcca
     2521 gcagggccgg acccgctgcg cgatgaatgg ccccatcgac gccgccacct cccattaatg
     2581 aaggattagc cgcattcaca atcacatcaa cggccagttt ggtaatatca ccctgcacaa
     2641 catgaatacg cgttttcata acgcctcctt acaccttctt gtttcttaag tgtacagcag
     2701 gctttttggt taaatcgcat ttttctgctc agcaacgtga gttgttcaga aacttagtta
     2761 tattcgatgt tcattacagc cagctttttc tgtttatcaa gatactgcac ttttactgag
     2821 gctatttgcg cgtttttgac attgccgctc attaacgctt tgctggagta ggttttaccc
     2881 ggaacagaac cattctgtgg gcagttcatt tctacgggtg atgactgggt gctgacgtca
     2941 catgccggtt ccacaatttg gccataaaaa tggatcacgc ccccttgcgc ggtgcttcct
     3001 gcgatggcaa taaaaggaaa aaacaaactg ccgagcataa gagagtttaa aaaaggtctg
     3061 aacatttatt ctatttcctc aatgattagt catccttgag ggttgtgtta tccatacttt
     3121 caggttggct gattcgcagc agaccattct ctccagattc atcttatgct cgatatttca
     3181 acaaattaag acttttctga agagggcggc cattgttgtg ataaatgaag tgactgtcca
     3241 tcagaaacag taacaactat tttcacccga tcgtccgggg aaatatttaa actcaacttc
     3301 gtcaaagcaa tgggttgatt agcaggcaat gaaagggtct tttcttgctt cgtctgactt
     3361 tgccctgaac tgccttcgcg cagggacaat atttgtactc tgcacagaca agattgagta
     3421 agagtgactt caggaataat ggtgtacata tccccttgct gggtcgtatt aaaggttatc
     3481 tgactggaaa gtgccgcaag gagtaataac gcattcatat tcttctcccg aaaaaaaaca
     3541 gggcttgcgc cctgtttctt taatacagat gatgtattag tactgatgag cggtcgcgtt
     3601 gttaccaaag ccaacctgag ttacgttgac ggtggaatta gatgcagtct ggtcaaccgc
     3661 tgcaccgttg ccgccaccga attgtttaac tgtcatatga gagtctttac cgttccactg
     3721 atcaagagtg gcgctgttac caaagccacg ttgggtcaga tcgattgagc tgtcatctga
     3781 gccctgacca acatctgcac cgttaccacc accatgctgg gtaatagtaa gatcagagtt
     3841 acgagcatca gcttgcagag caagtgcaga gttaccacca ccgtactgat aaatattcag
     3901 ctctgaattc gggccgctgt tattaccgcc accaccgtgg ttaccgccac cgccgccgta
     3961 ctgaggaaca acacctgcca gagcgctacc ggagaatacg attgctgcaa ttgctgctac
     4021 ttttaaaagt ttcatgtaaa acccccatcg gattgattta aaagtcgaat ggaaattaac
     4081 gttgtgtcac gcgaatagcc atttgcgact gtctctgcac tacaattgcc gttttttgag
     4141 taccatactg tgtaatattt gctttattac cagaaccttt ctggataatc atcgcagtat
     4201 taccataagc accttgcgaa atactggcat cattggcact gcccgcctga tcaatatatg
     4261 caaggttata atctcctgtc tggtcaatct ttgcccggtt gctactacct tcttgcgcaa
     4321 caaccgccaa aagttttgag cctccctgcc gtaactgagc actattatta gtcccagctt
     4381 gaccaattat ggctgcctga ttaaatgaag acttactcaa ttcatttacc gcgaagttat
     4441 attctgaatt agctaaatca taacctgctg cggctgcaat cccaggcgca cccagtattg
     4501 ttaacatcat aaataacaat ttgtttttca tgttgtcacc ctggacctgg tcgtacattt
     4561 aagaaa
//
LOCUS       DBFIFM010000280         4339 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.281, whole genome shotgun sequence.
ACCESSION   DBFIFM010000280 DBFIFM010000000
VERSION     DBFIFM010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.281"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(1..252)
                     /locus_tag="N7C74_004469"
                     /pseudo
     CDS             complement(1..252)
                     /locus_tag="N7C74_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
     gene            249..665
                     /locus_tag="N7C74_004470"
                     /pseudo
     CDS             249..665
                     /locus_tag="N7C74_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
     gene            793..1329
                     /gene="yfdR"
                     /locus_tag="N7C74_004471"
     CDS             793..1329
                     /gene="yfdR"
                     /locus_tag="N7C74_004471"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HGU2434194.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPSHA
                     YGMFMERFNELSELHKCA"
     gene            1320..1670
                     /locus_tag="N7C74_004472"
     CDS             1320..1670
                     /locus_tag="N7C74_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eae protein"
                     /protein_id="HGU2434195.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFDEVRAEARN
                     EGINYTASRLAAAFNHGFINKPLAEVFDVTRMILSAKEELANESHPIDGLSGEYAEKS
                     LEEWAERLRKGGSQ"
     gene            1667..2140
                     /locus_tag="N7C74_004473"
                     /pseudo
     CDS             1667..2140
                     /locus_tag="N7C74_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145689.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
     gene            2287..2484
                     /locus_tag="N7C74_004474"
     CDS             2287..2484
                     /locus_tag="N7C74_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434196.1"
                     /translation="MAELTKWLQNTITGIETVVDDKSFVCDEIVFKIDVVKNVLTAFK
                     VALVSLEVLMILCRSAMLSQN"
     gene            2442..2792
                     /locus_tag="N7C74_004475"
                     /pseudo
     CDS             2442..2792
                     /locus_tag="N7C74_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            2829..3110
                     /locus_tag="N7C74_004476"
     CDS             2829..3110
                     /locus_tag="N7C74_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HGU2434197.1"
                     /translation="MNTLFLLMAEFNTPNIELSAVSQKYFGMSPATAEAKANACKLPV
                     PTYRIGTSQKAKRCINIQDLAEYIDKRREEGRIEWEQVRTVKQKDKEHH"
     gene            complement(3158..3331)
                     /locus_tag="N7C74_004477"
     CDS             complement(3158..3331)
                     /locus_tag="N7C74_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434198.1"
                     /translation="MKDQKATKPQVKFDTMKAFAGMGAAVEVLMKAAPNAFTHATVSG
                     KEQQGKLRRRKAA"
     gene            complement(3528..4061)
                     /locus_tag="N7C74_004478"
     CDS             complement(3528..4061)
                     /locus_tag="N7C74_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434199.1"
                     /translation="MLTLDEIGQSVRNNIQLIIDHVGLPLAVGPLSDDDYKILCGGYG
                     ELEWDYALSTYGNSREKYEFCIKLVQQGRVQGIPSGAAICVYGVEENIFRIHMIERFS
                     REDESHPLKGRMVLLTLMSAFIFCKAVECKVVHIVEPVPELVQYYESFGFRMEQCGYV
                     MSAVIDELQDIFLKFAQ"
     gene            complement(4208..4312)
                     /locus_tag="N7C74_004479"
                     /pseudo
     CDS             complement(4208..4312)
                     /locus_tag="N7C74_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
BASE COUNT         1247 a          972 c          981 g         1139 t
ORIGIN      
        1 aatcgtgacg ttgttgagcg tcccggagtt cgcattcacg ttaccactga tatccgcatt
       61 tttagcagtc agctttccat ccggtgtcag ggaaaatgcc ggtggatttc caccactggt
      121 aatggtgggg gccgtcaggc gcttcaggaa cacgtcgttc atgaatatct ggttgccctg
      181 cgccacaaac atcggcgttt cattcccgtt tgccgggtca ataaacgcga tacgattggc
      241 ggcaaccaga atggattgaa gactgggccg actaccttgt gggctttgat gctaatggtg
      301 acaccattca ggcaacaaaa gcggctgcgg cggtccgtaa aatcacgatt gaagcaaacc
      361 agaccgctga ttttgaagat aatgacttca gcggcaaacg ctccctgatg gagtctgtcg
      421 aagcgaagac caaagacatt atgccagtgg catttgaatt taaatgcatt ccgtttgaag
      481 gtctgaaaga acgtccgttt aaattacgcc tcagcattat cactggcgat cgtccggtac
      541 tggttctgcg cattattcag ctggaggcgg tgcaggaaga aatggctaac gaatttcgtg
      601 atctgcttgt tgagaaattc aaggacagca aagtagaaac ctttattggt actttcaccg
      661 cctgatttca ttactgcaaa tgcccctgag ggggcattta tggaaacgta atttactcaa
      721 taatcgccgg atggtgaggg attcttttta ccagaattca gcgcggtgca gcgcatatac
      781 gtggagaaca aaatgtcatt tattaaaact ttttccggga agcattttta ttatgacagg
      841 ataaataaag acgacatcga tattaacgat atcgcggttt ccctttcaaa tatctgtcgc
      901 tttgccggtc atctttcgca cttctacagc gtcgcccaac atgcggttct ttgcagccag
      961 ctggtaccgc aggaatttgc ttttgaagca ttaatgcatg atgcaacaga agcgtattgc
     1021 caggacatcc ccgctccact gaaacgcctt cttcctgact ataaacggat ggaagaaaaa
     1081 attgacgccg taatccgtga gaaatacggg ttacccccag ttatgagtac gcccgtgaaa
     1141 tatgccgatc ttatcatgct ggcaaccgaa cgccgcgatc tcgggcttga tgatggctct
     1201 ttctggcctg tactggaagg catcccggca acagagatgt tcaacgtgat tccactggca
     1261 cctagccatg cctacgggat gtttatggaa cgttttaacg agttatcgga gttacacaaa
     1321 tgcgcatgaa tgttttcgaa atggaagggt ttcttcgcgg gaaatgtgta ccgcgagatc
     1381 tgaaagtgaa tgaaacaaat gctgagtatc tggtgcgtaa attcgatgaa gtacgtgctg
     1441 aggctcgcaa cgagggtatt aactataccg caagccgtct tgctgctgct ttcaatcacg
     1501 gatttatcaa taagcctttg gctgaagttt tcgacgttac acgcatgatt ctgtcagcaa
     1561 aagaagagtt agctaatgaa tcgcatccga ttgatggcct gtccggtgaa tatgcagaga
     1621 aatcccttga agaatgggcg gaacggcttc gcaaaggagg cagccagtga ctggacatgc
     1681 agcaatcctc gacatgtgct gtggcagtcg catgttctgg ttcgataaga atgacgaccg
     1741 ggcgatattt agcgatatca gaaaggaaga gcacacattg tgtgatggac gacgcctgat
     1801 tatcagtcct gatctgatag ctgattttcg tgcactacca tttgcagacg catctttttc
     1861 gatggttata ttcgaccctc cgcatcttga gcgtgttggt gataacgcct ggatgggaaa
     1921 gaaatatgga cggctgaata aagataccta gcgtgatgat ttgcggcagg gatttaaaga
     1981 agcctttcgt gtgttgcgtc catccggcgt tctgattttt aaatggaatg aaacgcaaat
     2041 acctgttcgc cagatattgg tactgaccga cagaaaacct gttatcggtc aacgaacagg
     2101 aaaaaacgat aaaacccact ggattatttt tatgaaatag gcatccagtg agtaggttcg
     2161 taaggttaca gatacgtata tctgaataat taaattcagt tctgtaaata aaatttaatc
     2221 cttaaccgga gggattcctg caccctcaga acatcaggag accgcccgaa agggcggtaa
     2281 tgaaaaatgg ctgaattaac caaatggcta caaaacacga ttaccggaat tgaaacggta
     2341 gtagacgata aatcgtttgt atgtgatgaa atagtattca aaatcgatgt ggttaaaaac
     2401 gtacttaccg catttaaagt cgcgctggta tcgctggaag tattgatgat actttgtcga
     2461 tcagcgatgc tctctcagaa ctaaaccatt tcattaaccg gcacgcagac aatacgaaat
     2521 atttaaaagt ctggggtaac ggggccacct tcgacaacgt aattttacgt ggagcttacg
     2581 agcgagcagg acaaatctgc ccgtgggcgt actggaatga ccacgatgta cgcacgatcg
     2641 ttacgcttgg gcgttccatc ggattcgacc ccaaaatgga catgcctttc gatggcgaac
     2701 ggcacaacgc cctggctgat gcccgtcatc aggcaaaata tgtttccgct atctggcaga
     2761 aattaattcc tgccaccagc acagaattat gattttcccg ggtgcagccg gttttgatgg
     2821 agaaaattat gaacaccttg tttttactga tggctgaatt caatacccca aacattgaac
     2881 tctcagcagt tagccaaaag tactttggta tgagtccagc cacggcagaa gcaaaagcaa
     2941 acgcttgtaa gttgcccgtt ccaacatatc gcatcggcac atcacaaaaa gcaaaacgtt
     3001 gcatcaatat tcaagatctt gcggaataca tagacaaaag gcgagaagaa ggacgtatcg
     3061 agtgggaaca ggtcagaaca gtcaaacaga aggacaaaga acatcactaa agaaaaaacc
     3121 cgcctgaagg cgggttttca aaaagcacca gctatgatca tgctgctttg cgacgacgaa
     3181 gcttaccctg ctgctcttta ccagagacag tagcgtgagt gaacgcatta ggagcagcct
     3241 tcatcagaac ttcaacagca gcacccatac ctgcgaatgc tttcattgtg tcgaacttaa
     3301 cctgtggctt ggttgctttt tgatctttca tagaaaactc ccgagacagt aaaggcgtct
     3361 ctaacccttt ctttaaagct agcttgtttc gctaacttat gccaatcgat catgtcgatt
     3421 ggtgacatcg tttcttagta gtttaagcac aaaacgactg ccatagatgt acctttaagg
     3481 taatctggac gggtatccta caatttgtag acccttctcg tctataccta ctgagcaaat
     3541 ttaagaaaga tatcctgcag ctcatcaatg actgccgaca tcacataacc gcactgttcc
     3601 atgcggaaac caaaagactc gtaatactgc accagttctg gtactggctc tacaatgtgg
     3661 acaactttac attcaacagc tttacaaaat ataaaagcac tcataagagt gagtaaaacc
     3721 atgcgccctt tcaatgggtg agattcatct tctctagaaa acctttcgat catatggata
     3781 cgaaagatgt tttcttcaac cccataaaca caaattgctg ctcctgatgg tattccctga
     3841 acccgacctt gctgaacaag ttttatgcag aactcatact tttctctgga gttgccatag
     3901 gtacttaacg catagtccca ttcaagctca ccatagccac cacacagaat cttgtaatca
     3961 tcatcactga gcggaccaac agcaagaggt aagccgacat gatcaataat caactggata
     4021 ttgttacgta ctgattgacc tatctcgtcc agggtaagca tcatggcctc tcaagcggaa
     4081 cactaaaagt cgcattatat ctcattctta agccgcgtat ggattacacc ttgaaatgaa
     4141 aacgccgggt tcccaatagg ctcccacaaa gtatataact atttgttttt caaaaacggt
     4201 acatcctatc gagcattggt gcaacgctaa accgaccact ccagtgaacg tcagtttttt
     4261 caggcattgc gctggtttgg ttgatttttt gcatttcaga attaccgtgc attttcaaat
     4321 gtagagatta ttttatcga
//
LOCUS       DBFIFM010000281         4282 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.282, whole genome shotgun sequence.
ACCESSION   DBFIFM010000281 DBFIFM010000000
VERSION     DBFIFM010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.282"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..228
                     /locus_tag="N7C74_004480"
     CDS             <1..228
                     /locus_tag="N7C74_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006176174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434200.1"
                     /translation="GTQQGAGQRESVMTRHRFCGINVAYEYNIPLSTCSTHQQILNWV
                     WHLTEKTWMTQNVTRRFIEVACGYHKLDYRQ"
     gene            324..422
                     /locus_tag="N7C74_004481"
     CDS             324..422
                     /locus_tag="N7C74_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004141699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434201.1"
                     /translation="MRPQSELLTLSQMQKCTCDVLHPAFDLCGGEA"
     gene            419..874
                     /locus_tag="N7C74_004482"
     CDS             419..874
                     /locus_tag="N7C74_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HGU2434202.1"
                     /translation="MNLPQDGIKLHRGNFTAVGQQIQTYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLISRGKSFATSAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLGTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            874..1044
                     /gene="ninE"
                     /locus_tag="N7C74_004483"
     CDS             874..1044
                     /gene="ninE"
                     /locus_tag="N7C74_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGU2434203.1"
                     /translation="MATPLIRVMNGHIYKVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            1037..1327
                     /gene="ybcO"
                     /locus_tag="N7C74_004484"
     CDS             1037..1327
                     /gene="ybcO"
                     /locus_tag="N7C74_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HGU2434204.1"
                     /translation="MADLRKAARSRECQVRIPGVCNGNPETSVLAHIRLTGLCGTGTK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            1324..1686
                     /gene="rusA"
                     /locus_tag="N7C74_004485"
     CDS             1324..1686
                     /gene="rusA"
                     /locus_tag="N7C74_004485"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HGU2434205.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKG
                     SMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGKLELTITELGNE"
     gene            1683..1823
                     /locus_tag="N7C74_004486"
     CDS             1683..1823
                     /locus_tag="N7C74_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HGU2434206.1"
                     /translation="MMFESYMAERLRHRWMRLRLYRFPGSVLTDYRILKNYAKTLKGA
                     AA"
     gene            1820..2509
                     /locus_tag="N7C74_004487"
     CDS             1820..2509
                     /locus_tag="N7C74_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HGU2434207.1"
                     /translation="MNTQYLQYVREQLIVATADLSGATKGQLEAWLEHAQFDTGTYKR
                     KKPRILDEVTGRMITLDNPPISGKQSYAKGSSIALVSQVEFSTSSWRRAVLSLEEHQK
                     AWLLWSYSESVRWEHQVTITQWAWSEFKTLLGTRKIAGKTLERLKKLIWLAAQDVKNE
                     LAGRKTYEYQELASLVGVTSKNWSETFTERWVAMKHIFLQLDSQALLLLTKTRSKQKT
                     TFSQQDIAKLD"
     gene            2831..3136
                     /locus_tag="N7C74_004488"
     CDS             2831..3136
                     /locus_tag="N7C74_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGU2434208.1"
                     /translation="MHEKESLAGAFWLVLLIIAGWGGLVRYLIDVKQSKATWSWINAL
                     AQIVVSGFTGVIGGLISIESGFSIYMILATAGISGAMGSVALTYFWERLTGVKNAKS"
     gene            3123..3599
                     /locus_tag="N7C74_004489"
     CDS             3123..3599
                     /locus_tag="N7C74_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HGU2434209.1"
                     /translation="MQNLNPQRKAFLDMLAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLHPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVRESEV"
     gene            3816..3998
                     /gene="rzoD"
                     /locus_tag="N7C74_004490"
     CDS             3816..3998
                     /gene="rzoD"
                     /locus_tag="N7C74_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HGU2434210.1"
                     /translation="MRKLKMMLCMMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(4089..>4282)
                     /gene="bor"
                     /locus_tag="N7C74_004491"
     CDS             complement(4089..>4282)
                     /gene="bor"
                     /locus_tag="N7C74_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HGU2434211.1"
                     /translation="ETITHHFFVSGIGQKKTVDAAKICGGAENVVKTETQQTFVNGLL
                     GFITLGIYTPLEARVYCSQ"
BASE COUNT         1142 a         1010 c         1083 g         1047 t
ORIGIN      
        1 ggcactcaac agggcgcagg tcaacgtgaa tcggtcatga cacgacaccg attttgcgga
       61 atcaatgttg cttatgaata caacatcccg cttagtacat gttcaacaca tcagcaaatt
      121 ttgaactggg tgtggcactt aactgaaaaa acatggatga cacaaaatgt tactcgtcgc
      181 ttcattgaag tagcttgtgg atatcacaag ctagactatc gccaatgata ttcaattttt
      241 gattttttag tatcaacctt ccataataat gtcaccggag cttgaacaac tccggtgact
      301 tctgcgcatt taaggggact taaatgcgac cacaatctga actccttacc ttgtcacaga
      361 tgcagaaatg cacctgcgat gtcttgcatc cagcgtttga tctctgcgga ggtgaagcgt
      421 gaacctccca caagatggca tcaaattaca tcgcggtaac ttcaccgctg tcggtcagca
      481 gatccagact tatctggaag acggcaaatg ctttcgcatg gtgcttaaac cgtggcgcga
      541 gagacgcagt ctttcccaga atgcactcag ccacatgtgg tacagcgaaa tcagtgaata
      601 cctcatcagc agggggaaat cgttcgctac ttcagcatgg gtaaaagatg ctctcaaaca
      661 cacatacctc ggttatgaaa ccaaggacct ggttgatgtc gtaaccggcg aaatcactac
      721 tatccagtcg ttacgccata cctccgatct tggtaccgga gagatgtatg tcttcctgtg
      781 taaggttgaa gcctgggcga tgaatattgg ctgccacctg actattccac agagctgcga
      841 gttccagctg ctgcgtgaca agcaggaggc gtaatggcta caccgcttat tcgtgtcatg
      901 aacggacaca tctacaaagt accaaatcgt cgtaagcgta aacctgagct gaagccatcc
      961 gaaataccaa cactgctcgg atataccgcc agcctggttg ataaaaaatg gttgcgactg
     1021 gcagcaagga ggaatcatgg ctgatttgag aaaagcagcg cgtagtcggg aatgccaagt
     1081 aagaatccct ggcgtatgta atggcaaccc tgaaacgtct gtactggcac atatccggct
     1141 gactggattg tgcggcaccg gtaccaaacc gccagacttg attgccacca ttgcatgttc
     1201 tgcctgccac gacgaaatcg accgccgcac acattttgtc gatgctgcat atgcaaaaga
     1261 atgcgcgctg gaaggtatgg cgagaacaca ggttatctgg ctgaaagagg gggttattaa
     1321 ggcgtgaata cctacagcat cacattaccc tggcctccga gcaataatcg ctactaccgg
     1381 cataatcgcg ggcgcacgca catcagcgca gaagggcagg cataccgtga taacgtcgcc
     1441 cgaatcatca aaggctccat gctggatatc ggcctggcta tgccagtgaa aatccgtatt
     1501 gagtgccaca tgccggatcg ccgtcgccgt gacctggata atctgcaaaa agccgctttt
     1561 gacgcactca ccaaagcagg tttctggctg gatgatgctc aggtcgttga ttaccgtgtt
     1621 gtgaagatgc ccgttaccaa aggtgggaag ctggagctga ccatcaccga actggggaat
     1681 gaatgatgtt tgagtcttat atggcagaac gtcttcgcca ccgctggatg cgcctgcgct
     1741 tatatcgttt tcctggttct gttttgaccg attaccggat actgaagaat tacgccaaaa
     1801 cactgaaagg agctgccgca tgaataccca atatttacag tatgtccgcg agcaactcat
     1861 tgtggctacc gctgatttga gcggagcaac gaaaggacag cttgaagcct ggctggagca
     1921 tgcacaattt gatactggta catacaaacg aaagaagccg cgcattctgg atgaggtaac
     1981 tggcaggatg attacgctgg ataatccgcc gatttccggt aagcagtcgt acgcaaaagg
     2041 ttcatccatt gcactggtca gccaggttga gttctcaacc tcgtcatggc gccgcgcggt
     2101 tctgtctctc gaagaacatc agaaagcgtg gttgctgtgg agttacagcg aaagtgttcg
     2161 ctgggaacat caggtcacca taacgcagtg ggcatggagc gagtttaaga ctctgttggg
     2221 taccaggaaa attgcaggta agacactgga acgtttgaag aagttgatct ggctggcggc
     2281 acaggatgtg aagaacgagc tggcagggcg taagacctat gaataccagg agctggcatc
     2341 actggtggga gtgacatcaa aaaactggtc tgagacattt actgaacgct gggttgcaat
     2401 gaaacacatt tttctacagc ttgatagcca agctttattg cttttaacga aaacacgttc
     2461 aaaacaaaag accacatttt cacagcaaga tattgcaaaa ctggattaaa aatcatatat
     2521 tttatgtaaa tctgatattt tgccaatgtt gtacgcactg gcagtaatcc aaattcaagc
     2581 ccgaggttta aagccttggg cttttctgtt tctgaacggt gagtagcctt ccaacctacc
     2641 ccagccaggg ggtcttcagc tgttgagttg atattgctta gccctctgtt gccagctaca
     2701 tgctggcttt tttattccag gcttgtgggg agcatcaact ccgtgctttg tcgttaaatt
     2761 accccgtgag cctgatttct gacatttaac gtcccggcct tttgtcggcg gcgaaacatt
     2821 ggctattcat atgcacgaaa aagagagcct tgccggagcg ttctggctcg ttttgctgat
     2881 catcgcaggt tggggcggtc tggtccgcta cctgatagat gtgaagcaga gtaaagcaac
     2941 gtggagttgg ataaatgctc tggctcaaat agtggtatca ggattcaccg gtgttattgg
     3001 tggcctgatc agcatcgaaa gtggattcag tatttacatg attctcgcga cagcggggat
     3061 tagtggtgcg atgggttcgg ttgcactgac gtacttctgg gaacgactga caggggtgaa
     3121 aaatgcaaaa tcttaatcct cagcgtaaag ccttcctcga tatgttggcg tggtcagaag
     3181 gaacggataa cgggcgacaa ccgacacgta accacggtta tgatgttatt gttggcggcg
     3241 aactgtttac tgattactcc gatcaccctc gcaaacttgt cacgctacac cccaaactca
     3301 aatcaacagc cgccggacgt tatcagcttc tttcacgctg gtgggatgct taccgtaaac
     3361 agcttggttt gaaagacttc tcccccaaaa gccaggacgc agtggcattg cagcagatta
     3421 aagagcgtgg tgctttacct atgattgatc gcggtgatat ccgtcaggca atcgaccgtt
     3481 gcagtaatat ctgggcttca ctgccgggcg ctggttatgg tcagttcgag cataaggctg
     3541 acagcctgat tgcaaaattc aaagaagctg gcggaacggt cagagagagt gaggtatgag
     3601 cagaataacc gcgattatct ccgctctgct catctgcatc atcgtctgcc tgtcatgggc
     3661 tgttaatcat taccgtgata acgccattac ctacaaagcc cagcgcgaca aaaatgccag
     3721 agaactgaag ctggcgaacg tggcaattac tgacatgcag atgcgtcagc gtgatgttgc
     3781 tgcgctcgat gcaaaataca cgaaggagtt agctgatgcg aaagctgaaa atgatgctct
     3841 gcatgatgat gttgccgctg gtcgtcgtcg gttgcacatc aaagcagtct gtcagtcagt
     3901 gcgtgaagcc accaccgcct ccggcgtgga taatgcagcc tccccccgac tggcagacac
     3961 cgctgaacgg gattatttca ccctcagaga gaggctgatc actatgcaaa aacaactgga
     4021 aggaacccag aagtatatta atgagcagtg cagatagagc tgcccatatc gatgggcagc
     4081 tcatgcaatt attgtgagca atacacacgc gcttccagcg gagtataaat gcctaaagta
     4141 ataaaaccga gcaatccatt tacgaatgtt tgctgggttt ctgttttaac aacattttct
     4201 gcgccgccac aaattttagc tgcatcgaca gttttcttct gcccaattcc agagacgaag
     4261 aaatggtggg tgatggtttc ct
//
LOCUS       DBFIFM010000282         4240 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.283, whole genome shotgun sequence.
ACCESSION   DBFIFM010000282 DBFIFM010000000
VERSION     DBFIFM010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.283"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            270..1520
                     /gene="frc"
                     /locus_tag="N7C74_004492"
     CDS             270..1520
                     /gene="frc"
                     /locus_tag="N7C74_004492"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HGU2434212.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPDWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            1574..3268
                     /gene="oxc"
                     /locus_tag="N7C74_004493"
     CDS             1574..3268
                     /gene="oxc"
                     /locus_tag="N7C74_004493"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HGU2434213.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            3338..>4240
                     /gene="yfdV"
                     /locus_tag="N7C74_004494"
     CDS             3338..>4240
                     /gene="yfdV"
                     /locus_tag="N7C74_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HGU2434214.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVV"
BASE COUNT         1118 a          921 c         1013 g         1188 t
ORIGIN      
        1 ttcttacagt tgtaagaata acatcacaaa tgtcgcgact ctctctgatt accacgaacc
       61 aatatttatc atattcttct tttcgtcctc actacttacc atgcagaaag gaattggaag
      121 tagcgatgaa atgattttca gcacttgata caggaattta aaaatatcga gtgccgctat
      181 gacgatattt tgcggattat ttatagaggg cgttatgcct taaataaacg ctaaattaac
      241 acatatttaa ttaactaata ggtattgcta tgtcaactcc acttcaagga attaaagttc
      301 tcgatttcac cggtgtgcaa tctggcccat cttgtactca aatgctggcc tggtttggcg
      361 ctgacgtcat taaaattgaa cgccccggcg ttggtgacgt aacgcgtcac cagctgcgag
      421 atattcctga tatcgatgcg ctttacttca ccatgcttaa cagtaacaaa cgttctattg
      481 agttaaatac caaaacagcg gaaggcaaag aggtaatgga aaagctgatc cgcgaagctg
      541 atatcttagt cgagaacttt catccagggg ccattgatca catgggcttc acctgggagc
      601 atattcaaga aatcaatcca cgtctgattt ttggttcgat caaagggttt gatgagtgtt
      661 cgccttatgt gaatgtaaaa gcctatgaaa acgttgctca ggcagcgggt ggcgcggcat
      721 ccactacggg tttttgggac ggtccgccgc tggtaagcgc tgcagcgttg ggtgacagca
      781 acaccggaat gcatttactg atcggtttac ttgctgcttt gctgcatcgc gaaaaaacgg
      841 ggcgtgggca acgagtcacc atgtcaatgc aggatgccgt attgaacctt tgccgcgtga
      901 aattacgcga ccagcagcgt ctcgataaat tgggttatct ggaagaatac ccgcagtatc
      961 cgaatggcac atttggtgat gcagttcccc gcggaggtaa tgcgggtggt ggcggtcagc
     1021 ctggctggat cctgaaatgt aaaggctggg aaactgatcc taacgcctat atttatttca
     1081 ctattcagga gcaaaactgg gaaaacacct gtaaagccat cggcaaacca gattggatta
     1141 ccgatccggc atacagtaca gcccatgccc gacagccaca tattttcgat atttttgctg
     1201 aaatcgaaaa atacactgtc actattgata aacatgaagc agtggcttat ttgactcagt
     1261 ttgatattcc ttgtgcaccg gttttaagta tgaaagaaat ttcacttgat ccctctttac
     1321 gccaaagcgg cagtgttgtc gaagtggaac aaccattgcg tggaaaatat ctgacagttg
     1381 gttgtccaat gaaattctct gcctttacgc cggatattaa agctgcgcct ctattaggtg
     1441 aacataccgc tgcggtattg caggagctgg gttatagcga cgatgaaatt gctgcaatga
     1501 agcaaaacca cgccatctga tattaacggg ggcttctggc tcccaattta taaaaatttc
     1561 gaggttatta atcatgtcag atcaacttca aatgacagat ggtatgcata tcatcgttga
     1621 agcattaaaa cagaataata ttgacactat ttatggtgtt gtaggtattc ctgtgacgga
     1681 tatggcgcgc catgcccagg cggaaggcat tcgttatatt ggttttcgtc atgagcagtc
     1741 ggcaggctat gccgctgcgg caagcggttt tcttacccaa aaaccaggga tctgcctgac
     1801 agtgtctgcg ccaggattcc tcaatggttt gaccgcattg gccaacgcaa cggtaaatgg
     1861 ttttccgatg atcatgatta gcggctccag cgaccgcgcg atcgtcgacc tacagcaagg
     1921 tgattatgaa gagctggacc aaatgaatgc ggcaaaaccg tatgccaaag cagcatttcg
     1981 cgttaatcag ccgcaggatc ttggcattgc attggcacgc gctatccggg tctcagtatc
     2041 gggtcgccct ggcggagttt atcttgattt gccagcaaat gtcctggccg cgacgatgga
     2101 aaaagacgaa gcgttaacca cgattgtaaa agttgaaaat ccgtcgccag cattattgcc
     2161 atgcccgaag tcagtcacta gcgcaatttc gcttttagca aaagctgaac ggccattaat
     2221 tatccttggc aaaggcgcgg cgtattcaca agctgacgaa cagcttcgtg aatttattga
     2281 aagtgcccag attccattcc tgccaatgtc tatggcgaaa gggatcctcg aagatacgca
     2341 tccactttct gcggcagctg cgcgttcgtt tgccctggca aatgctgacg ttgtcatgct
     2401 tgttggtgca cgactgaatt ggttactggc acacggtaaa aaaggatggg cggcagatac
     2461 acagtttatt caactggata ttgaaccgca ggaaattgac agcaaccgcc ccattgctgt
     2521 gccagtcgtt ggcgatattg catccagtat gcaaggtatg ctggcagagc tgaaacaaaa
     2581 cacatttacg actccactgg tatggcgcga tattttaaat atccacaagc agcaaaatgc
     2641 acaaaaaatg catgaaaaat taagtacaga tacccaacca ttaaattact ttaatgcatt
     2701 aagtgctgtg cgcgatgtat tgcgtgagaa ccaggatatt tatttagtta atgaaggtgc
     2761 aaataccctg gataatgcac gaaatattat tgatatgtat aaaccacgtc gtcgtctgga
     2821 ttgtggtacc tggggtgtca tgggcatcgg tatgggctat gccatcggtg ctagcgtgac
     2881 ctctggttct ccggttgtcg ccattgaagg tgatagtgct tttggtttca gtgggatgga
     2941 aattgaaacg atttgtcgat ataacctgcc ggtaacgatc gttattttta ataatggcgg
     3001 catctacaga ggagacggtg ttgatctcag tggcgctggt gcaccatcac caacagatct
     3061 gttgcaccat gcaaggtatg acaaattaat ggatgcgttt cgtggcgttg gctataacgt
     3121 caccacgaca gatgaacttc gtcatgcttt aaccaccggt attcagtcgc gcaaaccgac
     3181 cattattaat gtggtcatcg accctgcagc aggaactgaa agtggccata ttaccaaact
     3241 taacccaaaa caagtcgctg gtaattaaat actgacatcg tctcctcttg ccaactcatt
     3301 atgcgatggt gaggggaccc ttatcggaaa tactattatg ctaacatttt ttattggcga
     3361 tttattgcct attatcgtaa tcatgctgtt gggttatttt agcggcagac gagaaacttt
     3421 ttcagaagat caagcccggg catttaataa actggtatta aactacgcgc ttcctgcagc
     3481 cctatttgtt tctattaccc gggcaaacag ggaaatgatt tttgcggaca ctcgtctgac
     3541 ccttgtatca ctcgtggtta ttgtcggatg tttctttttc tcctggtttg gttgctacaa
     3601 attttttaaa cgtacccatg cagaagcagc tgtatgtgca ttaattgcag gttcacctac
     3661 cattggattc ctggggtttg cagttctcga tcctatttat ggtgattccg tatcaacagg
     3721 tttagtggta gcaattattt ctattattgt taacgcaatt actattccta ttggtctgta
     3781 tttgctgaat ccttcctcag gagcggatgg taagaagaat agtaatctga gcgcattaat
     3841 ttctgcggca aaggagccag ttgtatgggc acctgttctg gcaacgatcc tggtgttggt
     3901 tggggtaaaa attccggcag catgggaccc aacctttaat ctgattgcga aggctaactc
     3961 aggggtagcg gtattcgctg cggggttgac tctggctgca cataaattcg agttcagtgc
     4021 cgaaattgct tataacacct tcctgaagct gattctgatg ccactggcgc tgcttcttgt
     4081 tggtatggca tgtcatttga acagcgaaca tctgcagatg atggtactgg caggcgcatt
     4141 accgccggca ttctccggaa tcattattgc cagccggttt aatgtctaca cccgcactgg
     4201 tacagcgtca ttggctgtga gcgtactggg ttttgtcgtc
//
LOCUS       DBFIFM010000283         4209 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.284, whole genome shotgun sequence.
ACCESSION   DBFIFM010000283 DBFIFM010000000
VERSION     DBFIFM010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.284"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            227..835
                     /locus_tag="N7C74_004495"
     CDS             227..835
                     /locus_tag="N7C74_004495"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HGU2434215.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVTQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            933..2324
                     /gene="selA"
                     /locus_tag="N7C74_004496"
     CDS             933..2324
                     /gene="selA"
                     /locus_tag="N7C74_004496"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HGU2434216.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2321..4165
                     /gene="selB"
                     /locus_tag="N7C74_004497"
     CDS             2321..4165
                     /gene="selB"
                     /locus_tag="N7C74_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HGU2434217.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDPPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQCLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          970 a         1068 c         1210 g          961 t
ORIGIN      
        1 gctcatcctt gttttatcgg gttatttgtc tgcttccagg caaaacccta cagatttata
       61 agaaagcctt aagtgagata tccatcagat agaaacaatt aggaaaatat ttatatcacc
      121 atgataaata tgtgatttat ttggaagtaa caaaccatca acattttctg ccctttttcc
      181 catcaccagg ccatactgat atttttcctc gcacaaagga ctcggcatga aactcgtcgg
      241 tagctacacc agcccgtttg tacgcaaact ttctattctg ttgttagaaa agggcataac
      301 tttcgaattt attaatgaac tgccctataa cgcggacaac ggcgtgacgc aatttaaccc
      361 gttaggaaaa gtgccggtgc tggtgaccga agagggcgaa tgctggtttg attcgccgat
      421 catcgctgaa tatattgaat taatgaatgt cgctccggcg atgttgccgc gcgatccgct
      481 ggagtcgttg cgggtgcgca aaattgaggc actggcggat ggcattatgg atgccgggct
      541 ggtatcggtg cgtgaacagg cgcgtccagc ggcgcagcag tctgaagatg aattgttacg
      601 ccagcgggag aaaatcaacc gcagtctgga tgtgctggaa ggatatctgg tcgatggcac
      661 actcaaaacc gatacggtca atctggcgac tatcgccatt gcctgtgccg tcggatatct
      721 caatttccgc cgcgttgcgc caggttggtg tgtcgaccgc ccacacttag tcaaactggt
      781 cgaaaacctg tttagccgcg aaagttttgc tcgcaccgaa ccgccaaagg cttgatgcgc
      841 gatatgtcct cctgacccat ctcacgttac aatccgtggt tatgttaaac gcccttctcc
      901 gtgtgagagg gccttgatca gccaggtttc ctatgacaac cgaaacgcgt tccctctata
      961 gtcaacttcc ggctattgat cgcttattgc gcgatagctc cttcctttct ttgcgtgata
     1021 cttatggtca cacccgcgtg gtggaattgt tgcgtcagat gctcgacgaa gcgcgagaag
     1081 tgattcgtgg cagccagacg ctgcctgcgt ggtgtgaaaa ctgggcgcaa gaagtcgatg
     1141 cccggttgac gaaagaagcg cagagcgcgc tgcgtccggt gatcaacctg acgggaaccg
     1201 tgctgcatac caaccttggg cgagctttac aggcggaagc cgcggtggaa gccgttgcgc
     1261 aggctatgcg ttcgccagtg accctcgagt acgatctgga cgacgccgga cgcggacatc
     1321 gcgatcgggc gctggcgcag ctgctgtgcc gtattacggg ggcggaagat gcctgtatcg
     1381 tcaataacaa tgcggcggcg gtgttattga tgttggcggc cactgccagc ggaaaagagg
     1441 tggtggtatc tcgcggcgaa ctggtggaga ttggcggcgc gtttcgtatt cccgatgtta
     1501 tgcgtcaggc aggctgcacc ctacacgaag tagggaccac caaccgcacg cacgcgaatg
     1561 attatcgtca ggcggtgaat gaaaataccg cactgttgat gaaagtacat accagtaact
     1621 acagcattca ggggttcacc aaagcgatag atgaagcgga actggtggcg ctcggcaaag
     1681 agctggatgt tcccgtagtg actgatttag gcagtggctc gctggtcgat cttagccagt
     1741 acggtttgcc gaaagagcca atgccgcagg agttgattgc ggcgggcgtc agtctggtga
     1801 gcttctccgg cgacaagttg ttaggcgggc cgcaggcagg gattatcgtt ggtaaaaaag
     1861 agatgatcgc ccgcctgcaa agccacccgc tgaagcgtgc attacgcgcg gataaaatga
     1921 ccctcgcggc gctggaagcc acgttgcgtc tttatttaca ccctgaagct ctgagtgaaa
     1981 aattaccgac cctgcgcctg cttacccgca gcgcagaggt cattcaaatc caggcacaac
     2041 gtttacaggc tccccttgcc gcacattacg gcgcggagtt tgcggtacag gttatgccat
     2101 gtctttcgca gattggcagt ggttcgctgc cggttgatcg cctgccgagc gcggcattaa
     2161 cgtttacacc ccatgatgga cgcggtagcc accttgagtc attagccgcc cgctggcgtg
     2221 aattgccagt gccggtgatt ggtcgtattt atgacggacg attgtggctg gatttacgct
     2281 gccttgaaga tgagcaacgg tttttggaga tgttgttgaa atgattattg cgactgccgg
     2341 acacgttgac cacggcaaaa caaccttatt gcaggcgatt actggcgtaa atgctgaccg
     2401 tctgccggaa gaaaaaaagc gcggcatgac catcgatctc ggctatgcct actggccgca
     2461 gccggatggt cgcgtgcctg gttttatcga cgtacccggt catgaaaagt ttctttccaa
     2521 catgctggcg ggcgttggtg gtatcgatca cgcgctgttg gtggtggcgt gcgatgacgg
     2581 cgtgatggca cagacccgtg agcatctggc gattttgcag ctgaccggta acccgatgct
     2641 gacagtggcg ctgaccaaag ccgatcgcgt agacgaagcg cgtgttgatg aggttgaacg
     2701 ccaggtaaag gaggttctgc gggaatacgg ttttgctgag gcaaaactgt ttatcaccgc
     2761 agcaaccgaa ggtcggggaa ttgatgccct gcgcgagcat ctgcttcagt tgccggaacg
     2821 cgagcacgcc agccaacata gtttccgcct cgcgattgac cgcgcattta ccgtaaaagg
     2881 tgccgggctg gtcgtcaccg gtacggcgtt aagcggggaa gtgaaggtag gcgattcact
     2941 ctggctgact ggtgtaaata aaccgatgcg tgtacgtgcg ctgcatgcgc aaaaccagcc
     3001 aacagaaacc gccaatgccg ggcagcgtat cgcgcttaac atcgcgggtg atgcggaaaa
     3061 agagcagatt aaccgtggcg actggctgct tgccgatgtg ccgccagagc cgttcacacg
     3121 ggtgattgtc gagcttcaaa cccatacacc gctgacccag tggcagccgc tgcatattca
     3181 ccacgccgcc agccacgtca cgggacgcgt ttcactgctg gaagataacc ttgctgaact
     3241 ggtcttcgac cccccgttat ggctggcaga taacgaccgc ctggtattgc gcgatatctc
     3301 tgcccgcaac acgctggccg gagcgcgcgt cgtgatgctt aacccgccgc gtcgcggtaa
     3361 acgtaaacca gagtatctgc aatgcctggc ttccctcgcc agggcacaga atgatagcga
     3421 agcgttagcc gttcacctgc aacgcggcgc ggttaatctt cctgatttcg cctgggcgcg
     3481 ccagctcaac ggcgaaggga tgcgcgaact gctgcaacag cctggctata ttcaggctgg
     3541 ttatagcttg ttgaatgcgc cggttgccgc tcgctggcaa cggaaaattc tcgacacatt
     3601 agcgacttat catgagcaac atcgcgatga acctgggcct gggcgcgaac gtctgcgacg
     3661 tatggcgttg ccaatggaag atgaagcgct agtactgttg ctgattgaaa agatgcgcga
     3721 aagcggcgac atcctcagtc atcacggctg gctgcatctg ccggatcaca aagcgggctt
     3781 cagcgaagag cagcaggcca tctggcaaaa agcagagccg ctgtttggtg acgagccgtg
     3841 gtgggtgcgt gatctggcaa aagagacggg taccgacgag caggcaatgc gcctgacgct
     3901 acgccaggcg gcgcagcaag gaataattac cgcgatcgtc aaagatcgtt attaccgtaa
     3961 cgatcggatt gtcgaatttg ccaatatgat ccgcgatctc gatcaggagt gtggttcaac
     4021 ctgcgcggcg gatttccgcg atcgcttagg cgtaggccga aagctggcaa ttcagattct
     4081 ggaatatttt gaccgcattg gctttacgcg tcgtcgtgga aatgatcatt tattgcgcga
     4141 cgcattatta tttccggaaa aataaggaaa tgattaatta atttttaaaa taaattaata
     4201 caaatttct
//
LOCUS       DBFIFM010000284         4135 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.285, whole genome shotgun sequence.
ACCESSION   DBFIFM010000284 DBFIFM010000000
VERSION     DBFIFM010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.285"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..1361)
                     /gene="rhsD"
                     /locus_tag="N7C74_004498"
     CDS             complement(<1..1361)
                     /gene="rhsD"
                     /locus_tag="N7C74_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HGU2434218.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGIFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGMADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARKQHTASLSSPDTPRPL
                     SDSAFPDTLPGTEYGPDRGIRLSAVWLTHDPAYPESLPAAPLVRYTYTEAGELLAVYD
                     RSNTQVRAFTYDAQHPGRMVGHRYAGRPEMRYRYDDAGRVVEQLNPAGLSYHYQYEQD
                     RITVTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTE
                     YGL"
     gene            complement(1428..3536)
                     /locus_tag="N7C74_004499"
     CDS             complement(1428..3536)
                     /locus_tag="N7C74_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HGU2434219.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSIDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKNYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
BASE COUNT          859 a         1256 c         1065 g          955 t
ORIGIN      
        1 ttcagaccat actctgtccg ccgtcccgcc gcgtccgtct gcgccgttag ccttcctgcc
       61 gcgtcatacc cgctgtgcgt gacgctgccg tccgccagtt cttttttcac cacccgcttc
      121 agcccggccc cgccttctgt atgcagcacc tcacgccggt tcaggctgtc cgtgacggtg
      181 atgcggtcct gctcatactg gtagtggtaa ctcaggcctg ccgggttcag ttgctccacc
      241 acccgccccg catcgtcgta gcggtagcgc atctccggcc ttcccgcata acggtgcccc
      301 accatccggc ccgggtgctg cgcgtcatac gtgaaagcgc gcacctgcgt attgctgcgg
      361 tcatataccg ccagcagttc accggcttcc gtatacgtgt accgcaccag tggcgcagcg
      421 ggcaggctct ccgggtatgc cgggtcgtgc gtcagccaca ccgccgaaag gcggatacct
      481 ctgtcgggac cgtattcggt accgggcagt gtgtcgggga acgctgagtc tgaaagaggg
      541 cggggggtgt caggggaaga taaagaagcg gtgtgctgtt tacgggcctc ttccgcccgc
      601 tgcgcctgcg tggtcagcac cagacggaac tcccgcccgg caccgtccgt cacgccggtg
      661 atttccccgg ccaggtcacc ggcggcctca cgcctgtacg tcagcgtccg cccgaagcgg
      721 tccgccatcc cggtaagcac ccggtacggc ggcagcggcg ctggcagtac gtcctcagca
      781 cccggcaccc gctctgacca ccccagtatc caccacggcc cctgtgcgct gttggtcgcc
      841 aggtaaagat gcgggcttaa ccggatatcc ggcggcagcg ccccccacag ccgcgccagc
      901 gtgtggccgt ccggctgcgc tgccttacca ccgcgcacca gccacattga ctcgctgcgg
      961 ctgtacaccg cctcccccgg cagcagcggc tcaaagtgaa tgctccgccc gccgttgtca
     1021 ttgagtacca gtgcatcatc gcgtagctgt aagcggatat cagaaggcgc tttccagccg
     1081 gggccaaaaa tccccaccgg cgcaggcgtc cgggtccggt agctgctgta ggtgcgggag
     1141 agaatgaacg gcagcgggcc gggcagcgca aggtccgtct cgccgggcag caccttcgcc
     1201 cccagcagcg gatttaccgg gttgcccgaa gtcatcccgc ccgggcacac cgagcacgcc
     1261 acgccagttg gcgcgccaat tcttacacct gccgaaccct ggacaatggg accgccatac
     1321 tgagtcatat ctccctgacg cgccgctggt tttccgctca tgattcaatt ccttcttctg
     1381 tttattttta atgcttgttt gttactgaat accgaacagg tacgggatca gtactctctc
     1441 atatccggcg tatcctgctt ttcatcaggg gtcttcggtg gggatttcga ggctgcacag
     1501 ttccagttaa tcaacagtga gtcaccatta acctgctcct taccctgcaa atgaattttt
     1561 gtgccgttga gaaaaatttg tccgtccttc gtcagcacca gccttgcctt accacagcag
     1621 agctccagat gctcaccggc ggagtaaatc gcggtctgcc cggtatcatc ctttttcgtt
     1681 ttaccaacgg tgaaggtaac gttatttccg actttgacgt cataacccag cccgaccctc
     1741 aacgatttat gcaaaccgat ctgcgaggac tgcatcagag ccaccgaagt gttcataatg
     1801 ccacctaccg tcgtctggta cgccacgccc acggtcagcg cacgaacaag tccaatcgtt
     1861 tccacctgaa cagaacccac gttaatgatc tggttactgc ccaccgtctc tatctggtta
     1921 acgcccaccg tctgtatctg attgttggta accgcaatca tctgattgtt gccaatggtc
     1981 tcagcatggt tgtttatcac atccgtggtg cggttattca gcacctcggt gttcatgttc
     2041 ttctgcgcgt ggatgtagac ctgttctttc cctgtcgcat cgtcaaactt cagttcatta
     2101 aacccgctgc ccttatagtt tttcgaacga atggtcatct gcgtcttcgt ccccggcagg
     2161 ctgccgggtg tgcggttttc ctggtggtag gtacgcccca taataatcgg ctggtccgga
     2221 tcgccgttga ggaagtccac aatcacctcc tgacccacac gcggtatcgc caggttgcca
     2281 aagccggtgc ctgcccacgc ctgtgccaca cggatccagc atgaactgtc ctggtttgac
     2341 gggttatagc ggtcccagtt aaatttcacc cgcacgcgac catgttcgtc gcagaagatt
     2401 tcctcgcctg ccggtcccgt cacgacggcg ctctgcgggc catccaccag cggtttcagc
     2461 aacggctgtg gtcgccatgt ccggtctgcc ggtattaccg caaagtggtt attcagcgtg
     2521 gtacctgaac ccctgcgtcc cggcaccgcc tgtggctgct cgccgtgcag ttcacttgcc
     2581 accacctgcc attcccggtt caggttcgcc tgcggatgcc ccgtcagcac aattcgccgt
     2641 cccggccata tttccggaga acggcttgtt ccgcgcgcca cttctgcgtt gttgcgccag
     2701 ccatccatct gccagcgggc aaagttctgc ccgtgggcac ccttgaaacg tccggggtag
     2761 tcatacactt catactgtgt gcgctggtaa tcctggtact ggccttcctg atcaaaacgc
     2821 cctgcccagc cgggtcgttt aaaggtgtag tccttggtca ccacggaaga agggcggatt
     2881 tgtgcgctgt agcggaactg gctgatgcat agggtgctca cctcggtacg ggtgttcggg
     2941 ttccagggga tctcaaagga ctccggcaga taacgcacgg tgtcgcacag gaccaggctc
     3001 tggtcgatac ttttttgcgc gtgctcctca taaaagaaga tgccttcctc cgccgccatc
     3061 cggcacagga aatcgtaatc agtttcgccg tactggacac aaaactcacg ggaaggatgc
     3121 ggctcgctga acagcggact ccactcggtc acaccgtttt ctttcaggat cgtagcgagt
     3181 atgctttcga tgtcctcatt ctggaagata cggaagttct ggcgcagccc tgtgcgccac
     3241 agcggcgggc acaccttcat gctgtacagc atctggtttt tgtcgttctc ccccagttca
     3301 aaccaggtca ccacaccttt cacccggcgc tgtacgtcat cgccctgcca taccgtcagg
     3361 taggccattt tgtccagcac ctgcgcaaat tcaagggaaa gaaactgctg actgaccaga
     3421 gaaaggtcga gggaaaaaag tgaagagagt gactggttca gatgaaagga gaccaccgca
     3481 aacgcgtccg gtggcaggcc gtccacttcc agcgtgaaac gtaatccggt tgacatagca
     3541 acctccgtga aaatgaataa taatccgcac atattgcatg tacaacatgt cgggcggatg
     3601 aaataagtga gtcgacatct ggcgttaccg gggaatatca gaaaaatgtc atggttatgt
     3661 gatgatttag tctgggattt tacttcagag aaacgggtgt ctgtttagtc aacaaaataa
     3721 tcacaaacgc aacggatggg ttctcgacgt tattcacgac aggctcgtac attacagcga
     3781 gtaataaaaa acctgatatg caaagtcaaa ccaatattca taatgtcttt ttattcacaa
     3841 caataaataa tttaataaca atcattgaaa tgaatgatga tttgtataag gaatatacgc
     3901 taccgcccca ccctgataac atgatctgca cattgccatc ttaacgtcgt taaacactgg
     3961 cttaatagtg gatatttcaa attaggaatt tattaaattc acctgatgaa tatcataagc
     4021 aaatagcata gaaaataatg acaactcccc cgaggggacc atttccaaag cactgcacca
     4081 ccacctggtg cccggatgat cttgcttcag tggtaagagt acctgattca ttgtt
//
LOCUS       DBFIFM010000285         3923 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.286, whole genome shotgun sequence.
ACCESSION   DBFIFM010000285 DBFIFM010000000
VERSION     DBFIFM010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.286"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..185
                     /locus_tag="N7C74_004500"
     CDS             <1..185
                     /locus_tag="N7C74_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU2434220.1"
                     /translation="TPDEQKEPVAAIAPSLSTSTQTRVSASSCKVEFRHGNMTLENPS
                     PELLTMLIRELSGRGR"
     gene            182..532
                     /gene="tnpB"
                     /locus_tag="N7C74_004501"
     CDS             182..532
                     /gene="tnpB"
                     /locus_tag="N7C74_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU2434221.1"
                     /translation="MISLPSGTRIWLVAGVTDMCKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            563..2148
                     /locus_tag="N7C74_004502"
                     /pseudo
     CDS             563..2148
                     /locus_tag="N7C74_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            2177..2320
                     /locus_tag="N7C74_004503"
                     /pseudo
     CDS             2177..2320
                     /locus_tag="N7C74_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2431..2738
                     /locus_tag="N7C74_004504"
                     /pseudo
     CDS             2431..2738
                     /locus_tag="N7C74_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2735..3923)
                     /gene="pitB"
                     /locus_tag="N7C74_004505"
                     /pseudo
     CDS             complement(2735..3923)
                     /gene="pitB"
                     /locus_tag="N7C74_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
BASE COUNT         1021 a         1024 c         1057 g          821 t
ORIGIN      
        1 taacgccgga tgagcagaaa gaacctgtgg cggccattgc gccgtcttta tccacttcca
       61 ctcagaccag agtcagtgcc agttcctgca aggtggaatt ccgtcacggt aacatgacgc
      121 tggaaaatcc atcgccagag ctgctcacga tgttaatccg cgaactgagc gggaggggaa
      181 gatgatttca ctcccatcag gtacccgtat ctggctcgtt gccggcgtta ccgatatgtg
      241 taaatccttc aacggactgg gagaacaggt acaacatgtg ctgaatgata atcccttctc
      301 cggtcacctg tttatcttcc gtggccgacg gggggacacc gttaaaattc tttgggctga
      361 tgctgatggt ctgtgcctgt tcaccaaacg cctggaggaa ggccagttta tctggcctgc
      421 ggtgcgtgac ggtaaggtat ccattacccg ctcgcaactg gcaatgctcc tcgataagct
      481 ggactggcgt cagccaaaaa catcccgcct taacgccctg acaatgttgt aaaaaacacc
      541 tggccgcatt ataaaaacgg ccatgagtca gaaatacctc attcgcattg cagaactgga
      601 aagccagctc cgtcagaaag accagcaact gagtctggtt gaagagacgg aggccttcct
      661 gcactggcac gtgccgaaga aaagatcgaa gaagatgaac gggaaataga acatctgcgg
      721 gctcagatag aaaaactgcg ccggatgctg ttcggtaccc gttctgaaaa actgcgtcgt
      781 gaagttgaac aggctgaggc cctgctgaaa caacgcgaac aggacagcga tcgttacagt
      841 gggcgggaag acgatccaca ggttccccgc cagttgcgac agtcgcgcca tcgtcgtccg
      901 ttaccggcac accttccccg tgaaatacag cgtctggaat ctgaagaaag ctgttgcccg
      961 gagtgtggcg gtgagctgga ttatctgggg gaagtcagtg cagaacaact ggaactggtg
     1021 agcagcgccc tgaaagtgat ccgcacagaa cgggtaaaaa aagcgtgtac aaaatgtgac
     1081 tgcatcgttg aagcaccggc gccgtcccgc ccgatagagc gtggtatcgc gggccccgga
     1141 ttacttgccc gcgtgttaac gggaaaatac tgcgaacacc tgccactgta tcgtcagagt
     1201 gaaatctttg cccgacaggg tgtcgaactg agccgtgcat tactctccaa ctgggttgac
     1261 gcgtgctgcc agttaatgac gccgctgaat gatgccctgt accgttatgt gatgaatacc
     1321 cgcaagcttc acactgacga cacaccggta aaggtactgg caccgggcct gaaaaagacg
     1381 aaaacagggc gcatctggac gtatgtccgg gatgatcgca atgcgggttc gtcatctcct
     1441 ccggcggtct ggttcgcgta ctcaccgaac cggcagggga aacacccgga gcaacacctc
     1501 cgccccttcc ggggtatcct gcaggcggat gcgttcacag gttatgacag gctgttcagt
     1561 gcagaacgtg aaggtggtgc gctgacagaa gttgcgtgcc gggcccatgc ccggagaaaa
     1621 atccacgatg tatacatcag cagcaaaagt gcgacggcag aagaagccct gaagcgaatc
     1681 agtgaactgt acgccatcga ggatgaaata cggggattac cagagtcaga gcgtcttgca
     1741 gtcagacagc agcgaagcaa agcgttactg acgtcgctgc atgaatggat gatggagaag
     1801 aatggcacgc tgtcgaaaaa atccagactg ggcgaagcgt tcagctatgt actgaatcag
     1861 tgggacgccc tctgttatta cagtgatgac ggactggcgg agacggacaa taacacagcg
     1921 gaaagagcgc ttcgtgcagt ctgtcttgga aagaaaaatt acgtgttctt cggtagcgat
     1981 cacggcggcg agcgtggtgc actgctatac gggctgatcg gcacctgccg tctgaacggt
     2041 atcgatccgg aagcgtatct gcgccatatt ctgagcgtac tgccggaatg gccctccaac
     2101 cgggttgatg aactcctgcc atggaacgta gtactcacca ataaataagc gtcaatacgg
     2161 tgctccgttg acgcttacct tctttcattc gctgaaggtg gaatgtatcc acggggaacg
     2221 ctttagcagc cgggaaataa tgcgggaaac ggtgtttaat tatatcgagt gtgattacaa
     2281 tcgctggcgt cgtcacagtg cctgtggcgg tctcagcccg gactgtcttg cactgcaaat
     2341 attcagaaat gagtctatgc agttcaccag taaaatcagc catcagagag ggaatgtagt
     2401 ggcacagtaa atttggccac ctgattaaag gtgatatcct caccacaaca caaaacaggt
     2461 gacttaatga acaagaaaac caaacgtact ttcacccctg aattcaggct ggaatgtgca
     2521 cagctaattg ttgataaggg ctactcatat cgacaagcca gtgaagcgat gaatgtcggt
     2581 tctaccacgc ttgagagttg ggtgcgccag ctcaggcgag agcgtcaggg gattgcgccc
     2641 tctgccacac ctattactcc agaccagcaa cgtatccgcg aactggaaaa gcaggttcgc
     2701 cgcctggagg aacacaatac gatattaaaa aaggcttctc accgatggtc atcgcaacac
     2761 gacgccagcc aatcatggtg ccaatgccga gcgccagtgc tactgccatg atgatccaca
     2821 ccggagcgta ctcaatggtg cttaacatat cgctgcgcag ttttttcagc aggttctggt
     2881 cttctttact gacgcctggc agtttcgcca gcttcgcgga agtatcagag atgcacagca
     2941 taatgcggcg cagctggctg cgctgactca cgcttaacgg ctcgtaactt tccatattgc
     3001 ctggcagcat cgttttaacg cgcgcaatcg catcaaaggt atttcccgga tgacagtgaa
     3061 actccgtcac ttgcgcgcca tcagtagatg ccgcaggcaa tggaggttcc atcgcaatca
     3121 acttctgcgg cagttcagga tgctgttgca ggtagtgttc gaagttggta acggcatcgc
     3181 gggtacgggt aatttcatag ccggacgcgt tcatattgac gacgaagcca gcaggggcaa
     3241 tccccaccag taccagcatc accaggccga tccctttttg tccgtcgttc gcgccgtgcg
     3301 aaaacgccac gcccgcagcg gaaacaatca gcgcaatacg cgtccagaat ggcggcttac
     3361 gtttgccttt tttctttttg cgatcttccg gaatgcggtg aatacggtca cgctttttcg
     3421 ttccgctcca gtagcgtcgc agcaggaata tcaggcctcc cgcaatgacc aggccgacga
     3481 taggggaaac aatcagcgag gagaaaattt tggtcacttc acgcaggttt aacgcatcca
     3541 tcaccgatga gccggttaac agcgcgttgg ttaaaccgat gccgataatc gcgccaatca
     3601 aggtatgcga actggaggcc ggtaaaccaa agaaccacgt tcccaggttc cagataatcg
     3661 ccgccagcag catggaaaag accatcgcca ggccgtgggt tgaccccata ttcagcaaca
     3721 aatcggttgg caacatatgg acaatggcat aggcaacgct aagtccgccc aataacacgc
     3781 caaaaaagtt aaaaaatgcc gccatcacca cggcaagctg tggctgcatg gcacgagtat
     3841 aaataacggt tgccaccgca ttcgccgtgt catgaaaacc attgattgct tcgtagaaca
     3901 acacaaatgc cagagcaagt aat
//
LOCUS       DBFIFM010000286         3920 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.287, whole genome shotgun sequence.
ACCESSION   DBFIFM010000286 DBFIFM010000000
VERSION     DBFIFM010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.287"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..750
                     /locus_tag="N7C74_004506"
     CDS             <1..750
                     /locus_tag="N7C74_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU2434222.1"
                     /translation="TDAGAGFEQTLTGMTVGIDSRNDIPEGIATLGAFMGYSHSHIGF
                     DRGGHGSVDSYSLGGYASWEHESGFYLDGVVKLNRFESNVAGKMSSGGAANGSYHSNG
                     LGGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELGATL
                     SYNMRLGNGMEVEPWLKAAVRKEFVDDNRVKVNSDGNFVNDLSGRRGIYQAGIKASFS
                     STLSGHLGVGYSNGAGMESPWNAVAGVNWSF"
     gene            complement(1122..1388)
                     /gene="minE"
                     /locus_tag="N7C74_004507"
     CDS             complement(1122..1388)
                     /gene="minE"
                     /locus_tag="N7C74_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HGU2434223.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1392..2204)
                     /gene="minD"
                     /locus_tag="N7C74_004508"
     CDS             complement(1392..2204)
                     /gene="minD"
                     /locus_tag="N7C74_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HGU2434224.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2228..2923)
                     /gene="minC"
                     /locus_tag="N7C74_004509"
     CDS             complement(2228..2923)
                     /gene="minC"
                     /locus_tag="N7C74_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HGU2434225.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3443..3811
                     /locus_tag="N7C74_004510"
     CDS             3443..3811
                     /locus_tag="N7C74_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HGU2434226.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLSEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
BASE COUNT          996 a          884 c          964 g         1076 t
ORIGIN      
        1 actgatgcgg gtgccgggtt tgagcagacg ctgaccggaa tgacagtggg gatcgacagc
       61 cgtaatgata ttcctgaggg gattgcgacg ctgggcgctt ttatgggtta ttcccattca
      121 catatcggtt ttgatcgtgg aggacatggc agtgtggaca gttattctct gggcggctat
      181 gccagttggg aacatgaaag tggtttctat ctggacggtg tcgtgaagct gaaccgtttt
      241 gaaagtaacg tagccggtaa aatgagcagc ggtggagccg ccaatggcag ttaccatagc
      301 aacgggctgg gcggtcacat tgaaaccggg atgcgattta ccgatggtaa ctggaacctg
      361 acgccgtatg cctcgttaac ggggttcacc gctgataacc ccgaatatca tttatccaat
      421 ggcatggaat cgaaatcagt cgatacccgc agtatatatc gtgaactggg tgcaacgctg
      481 agttacaaca tgcgtctggg gaacggtatg gaagttgagc cgtggctgaa ggcggctgtg
      541 cgcaaagaat ttgtcgatga taaccgggtg aaagtgaata gtgacggtaa tttcgtcaat
      601 gatttgtcgg gcagacgtgg aatataccag gcaggtatta aagcctcatt cagcagtacg
      661 ttaagcgggc atcttggggt ggggtatagc aacggtgctg gtatggaatc cccgtggaac
      721 gcggtggctg gtgtgaactg gtcgttctga ccatcaacga ttaaactgcg cttcggcgca
      781 gttttcgttt acaggatgtt aaaagagaaa attctggggt aaaaaaagcc cgccagttac
      841 ggcgggaaac ctcatcctat gggagaacaa tgaataatga aattgcgggg ttatcatctc
      901 ccagtatatc aacactaaca ataaggttat ttactcaacc aggcataaat attttgtttt
      961 gtgcgtggga acagccttaa ggtgtaaagg gggaggtgga aatagcaatg aggagtatca
     1021 gcaagaatac tcgccgcttt accacaacgt ggatgagagg gatgaaaaac tcaaggcaga
     1081 gataactctg ccttgaagat aaatgcgctt ttacagcggg attatttcag ctcttctgct
     1141 tccggtaagg tcacgttcag ctcaagaata gaaatatcgc catctttttg ctcaagctgt
     1201 acggttacca tctcaggatc aatttgtacg tatttacaaa tgacctcaag aatatcttta
     1261 cgcaactgcg gcagataatg cggttctgca tcgctgcgac ggcgttcagc aacaataatc
     1321 tgcagccgtt cttttgcaat gttggctgta tttttcttcc gcgagagaaa gaaatcgagt
     1381 aatgccataa cttatcctcc gaacaagcgt ttgaggaagc ctttcttctc ttcttcaatg
     1441 aagcggaaag gacgttcttc tcccaacaga cgttctacgg tatctgcgta ggctttaccc
     1501 gcatcggcgt taatgtcgag aatgaccggt tcaccctggt tagaggcgcg caatactgat
     1561 tgatcctctg ggatcacgcc gacgagtttg atgcgcagga tctccagcac atcttccatg
     1621 ctcagcatgt cacctctgct tacgcggcct gggttatagc gcgttaacag caggtgctct
     1681 ttaataggct cttcgccatt ttctgcgcgg cgtgattttg acgccagaat gcctaaaata
     1741 cggtcagagt cgcgtactga ggagacttcc gggttggtgg taataatggc ttcgtctgca
     1801 aaatagagtg ccattaacgc accggtttca atccctgccg gggagtcaca aacgataaat
     1861 tcaaaatcca tcgctttcag atcatcaaga actttagcga ccccttcacg ggtgagggca
     1921 tctttatcgc gtgtttgcga tgccggcaga atatagagat tttcagtacg cttatcttta
     1981 attaacgcct gatttagcgt tgcatcgccc tgaatgacgt tgacgaaatc gtaaacgacc
     2041 cggcgttcac aacccataat caggtcgaga ttacgcaggc cgatatcaaa atctatcacg
     2101 acagttttct ttcccttctg ggccaaacca gtggcgatgg ccgcgctgga ggttgtctta
     2161 ccaacacccc ctttgcccga agtaacaaca ataatgcgtg ccatagaaat tccttgttaa
     2221 aaagggatca atttaacggt tgaacggtca aagcgttttc gactaactgc agtcgcgccg
     2281 ctttgccata aaattctgct gggatttgat cactcagcca gtattcacct gcgatggaca
     2341 ccaattccgc catcaggttc gtacaaaata tttgcgtttc ccggtcacca cttgcccctg
     2401 ccagcgcacg accgcgcatc atgccataga catgaatgtt cccatcggca atcaattcgg
     2461 ccccagcgct aacgtggctt gtaacaatca gatcacattg tggagcataa atacgctgac
     2521 cggaacgcac cggggtatct attaaacgcg tttttgtgac cggcgttgta ttttgcgctg
     2581 gagcctgcgg tgcgggagct ggacgtggcg ctttttcctt tccttccgtc aggataggca
     2641 gccccatttt ttcaatttcg gctttaagtt gcgcatcttt gcagccactt acgccaataa
     2701 cccgcaaacc ggttgccgaa accgccttat gcatcgctga ccagtttacc gggtcttcca
     2761 gtgcactgac gttgagtaca acgggggcat gttttaaaaa tgcgggggcc tgagcgattt
     2821 tgtcttccag cgcctgatgg ataaccttag gttctgcctc atgcagatga accacagata
     2881 aagtgaagct actgccttta agctcgattg gcgtgtttga catcctggcc ttactcaatt
     2941 agctattaat catcgccagc gcgcgatgat gttccgaaga ctataaggca tgttatagtc
     3001 tggattatat tgaggcaagt caccctccca tttattcaga gtaaaagtct attctgtgat
     3061 aaatggcgct gattcatagc ttaaaaaata cccttgtcaa tcaacccatt gccgtcgtac
     3121 ttttgattct tcttatttac gcttcctttt tccgcaccct aatcgtgaca gcatctccag
     3181 acctgtttaa atatccagta ttgaggcaag tgtatttttt caccacgcca ggctttctta
     3241 actttccatt ttttgcagca tcattaaaga acagataccc gttatcttaa tatccgccac
     3301 agaattgctc tatcatagaa accgcagaag acgtctgata cttctgtcag gtcatcgtaa
     3361 ctcgtccagg tcagcggttt tgattacgga ttgatgatga aatgagcata agtgttgtac
     3421 ggttggatta tcaggatgtg ctatgaatat gatgcgtatt ttttatatcg gattgtcagg
     3481 tgtggggatg atgttctcat caatggcgtc tggacatgac gctggcggac ttcaatctcc
     3541 ggcgtgcggc gttgtttgcg acccgtatat ttgtgtgaac tcagatggca tttccccaga
     3601 gttaacaagg aaatatctca gcgaaaaagc cgctgaaaac ttacaatcat tacaaggcta
     3661 cgatcccagc gaatttacat tcgctaacgg tgtattttgc gatgttaaag aaaaattatg
     3721 ccgtgatgat cgttattttg gtgtagatgg taagcggagt ggaaaaatca atcaaaccac
     3781 cacaaagatg ttatttatgt gtcgtgagtg atgtcttgtt tacattatgt tctattcgtc
     3841 attaacctct tgagcgacag agaggagtgg ggcgtcaacg tttatgacgg ggagactccc
     3901 cgtcggttga catttactta
//
LOCUS       DBFIFM010000287         3894 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.288, whole genome shotgun sequence.
ACCESSION   DBFIFM010000287 DBFIFM010000000
VERSION     DBFIFM010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.288"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..520
                     /gene="ydeO"
                     /locus_tag="N7C74_004511"
     CDS             <1..520
                     /gene="ydeO"
                     /locus_tag="N7C74_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HGU2434227.1"
                     /translation="SNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFATCKGFI
                     TLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQTTFSQILL
                     DARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYRCQRHTG
                     MNTGNTMNALAI"
     gene            924..2606
                     /gene="ydeN"
                     /locus_tag="N7C74_004512"
                     /pseudo
     CDS             924..2606
                     /gene="ydeN"
                     /locus_tag="N7C74_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            2658..3815
                     /gene="ydeM"
                     /locus_tag="N7C74_004513"
     CDS             2658..3815
                     /gene="ydeM"
                     /locus_tag="N7C74_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HGU2434228.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKLGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNDETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3781..>3894)
                     /locus_tag="N7C74_004514"
     CDS             complement(3781..>3894)
                     /locus_tag="N7C74_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434229.1"
                     /translation="HSTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
BASE COUNT         1194 a          810 c          777 g         1113 t
ORIGIN      
        1 ttcgaatgta ccgactggat tgcttaatga aatgattgct tatctcaact cggaagaacg
       61 caatcatcat aatttttcag aacttttgct tttttcttgc ctgtctattt ttgccacatg
      121 caaaggtttc attacactat taactaacgg tgtgctatcc gtttcgggga aagtgagaaa
      181 tattgtcaac atgaagctgg cgcacccatg gaagctgaaa gatatttgtg actgcctgta
      241 catcagtgaa agcctgttga agaaaaaact aaagcaagag caaacgacat tctcacagat
      301 tcttttagat gccagaatgc agcacgcaaa aaatttgata cgcgtagaag gttcagtcaa
      361 taaaattgcc gaacaatgtg gttatgccag tacatcttat tttatttatg cgttccgcaa
      421 acatttcggc aacagtccaa agagagtttc taaggagtac cgttgtcaaa ggcacacggg
      481 tatgaatacg ggcaacacga tgaatgcttt agctatttga ttatttgcta acgagtagtc
      541 aaccacgcac cctacgtaaa aattaaaaat ggggcagtca ttccctgccc cgcgttgttt
      601 ttaggcgata tatttattga aataaataag tgacattcat cacatattta tgcatttgca
      661 taacttgttg catgattatt tatgatctca attcggcatt ttgttagtaa aatacaataa
      721 tttattaaat atcaatagat taattgttta tcgtcgagaa attacttagt agaacaaaaa
      781 gtaatgtcaa cgctttatgg actgttattt cccttttttt agctaaatct gctatctctt
      841 tatgtgacta acttcactta catccactta tttctcttcg taaaattact ttggaattaa
      901 gtacaataag aagaggaaca tttatgaagt ctgcattaaa gaaaagtgtc gtaagtacct
      961 cgatatcttt gatactggca tctggtatgg ctgcatttgc tgctcatgcg gcagatgatg
     1021 taaagctgaa agcaaccaaa acaaacgttg ctttctcaga ctttacgccg actgaataca
     1081 gtaccaaagg aaagccaaat attatcgtac tgaccatgga tgatcttggt tatggacaac
     1141 ttccttttga taagggatct tttgacccaa aaacaatgga aaatcgtgaa gttgtcgata
     1201 cctacaaaat agggatagat aaagccattg aagctgcgca aaaatcaacg ccgacgctcc
     1261 tttcattaat ggatgaaggc gtacgtttta ctaacggcta tgtggcgcac ggtgtttccg
     1321 gcccctcccg cgccgcaata atgaccggtc gagcgcccgc ccgctttggt gtctattcca
     1381 atactgatgc tcaggatggt attccgctaa cagaaacttt cttgcctgaa ttattccaga
     1441 atcatggtta ttacactgca gcagtaggta aatggcactt gtcaaaaatc agtaatgtgc
     1501 cggtaccgga agataaacaa acacgtgact atcatgacaa cttcaccaca ttttctgcgg
     1561 aagaatggca accgcaaaac cgtggctttg attactttat gggattccac gctgcaggaa
     1621 cggcatatta caactcccct tcactgttca agaatcgtga acgtgtcccc gcaaaaggtt
     1681 atatcagcga tcagttaacc gatgaggcaa ttggcgttgt tgatcgtgcc aaaacacttg
     1741 accagccttt tatgctttac ctggcttata atgctccgca cctgccaaat gataatcctg
     1801 caccggagca atatcagaag caatttaata ccggtagtca aacagcagat aactactacg
     1861 cttccgttta ttctgttgat cagggtgtaa aacgcattct cgaacaactg aagaaaaacg
     1921 gacagtatga caatacaatt attctcttta cctccgataa tggtgcggtt atcgatggtc
     1981 ctctgccgct gaacggggcg caaaaaggct ataagagtca gacctatcct ggcggtactc
     2041 acaccccaat gtttatgtgg tggaaaggaa aacttcaacc cggtaattat gacaagctga
     2101 tttccgcaat ggatttctac ccgacagctc ttgatgcagc cgatatcagc attccaaaag
     2161 accttaagct ggatggcgtt tccttgctgc cctggttgca agataagaaa caaggcgagc
     2221 cacataaaaa tctgacctgg ataacctctt attctcactg gtttgatgag gaaaatattc
     2281 cattctggga taactaccac aaatttgtcc gtcatcagtc agacgattac ccgcataacc
     2341 ccaacactga ggacttaagc caattctctt atacggtgag aaataacgat tattagcttg
     2401 tctatacagt agaaaacaat cagttaggtc tctacaaact gacggatcta cagcaaaaag
     2461 ataaccttgc cgccgccaat ccgcaggtcg ttaaagagat gcaaggcgtg gtaagagagt
     2521 ttatcgacag cagccagcca ccgcttagcg aggtaaatca ggagaagttt aacaatatca
     2581 agaaagcact aagcgaagcg aaataactaa accttcatgc ggcggatttt tccgccgcct
     2641 tattgagcga gatagcgatg cacgttacag ccaagccctc cagttttcaa tgtaatctca
     2701 aatgtgatta ctgtttttac cttgaaaaag agtcgcagtt tactcatgaa aaatggatgg
     2761 atgacagcac tctgaaagag ttcatcaaac aatatatcgc agcgtctggc aatcaggtct
     2821 attttacctg gcaaggcggt gaacccactc tggctggcct ggattttttc cgtaaagtta
     2881 ttcactatca acaacgctat gcaggccaaa aacgtatttt taatgcatta caaacgaatg
     2941 gcattttatt gaataatgaa tggtgtgcct ttctcaaaga acatgagttt ctggttggta
     3001 tctcgatcga tggaccccag gagctacatg accgttacag acgcagtaat tcaggtaacg
     3061 gtacttttgc aaaagtgata gcagccatcg agcgtctgaa atcatatcaa gtagagttta
     3121 atacgttaac cgtcattaat aacgttaatg tccattaccc tcttgaggtt tatcattttt
     3181 taaaatctat cggcagtaaa catatgcaat ttatcgaatt gctcgaaacc gggacgccga
     3241 atattgattt cagtggtcat agtgagaaca cattccgtat cattgatttt tctgtgcctc
     3301 ccacggctta tggcaagttt atgtcaacca tttttatgca atgggttaaa aacgatgtgg
     3361 gtgaaatttt catccgtcag tttgaaagct ttgtcagccg ttttttgggg aatgggcata
     3421 ccagttgtat tttccaggag tcctgcaagg ataatctggt tgttgaaagt aatggagata
     3481 tttacgaatg cgatcatttt gtctatccac agtacaaact tggaaacatt aataaatctg
     3541 aacttaaaac gatgaacagt gtacaactga cagcgcaaaa aaaacggatt tcagcgaaat
     3601 gtcagcaatg tgcatataaa cctatctgca atggcggttg ccctaagcat cgtattacta
     3661 aagtaaacga tgagactgtt tcctattttt gcgaaggtta taaaatcctt ttttcaacca
     3721 tggtacctta tatgaacgcc atggtggagt tagccaagaa cagagtaccg ctttaccaca
     3781 ttatggatgt tgcaaaacaa atggagaata attaataacg ctctctttta gctccattct
     3841 tagcctcctt cttatgacct taacaaggca cccgacataa attcctgtcg agtg
//
LOCUS       DBFIFM010000288         3879 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.289, whole genome shotgun sequence.
ACCESSION   DBFIFM010000288 DBFIFM010000000
VERSION     DBFIFM010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.289"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..827)
                     /locus_tag="N7C74_004515"
     CDS             complement(<1..827)
                     /locus_tag="N7C74_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU2434230.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLG"
     gene            complement(883..1215)
                     /locus_tag="N7C74_004516"
     CDS             complement(883..1215)
                     /locus_tag="N7C74_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGU2434231.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(1225..2544)
                     /locus_tag="N7C74_004517"
     CDS             complement(1225..2544)
                     /locus_tag="N7C74_004517"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGU2434232.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(2525..>3879)
                     /locus_tag="N7C74_004518"
     CDS             complement(2525..>3879)
                     /locus_tag="N7C74_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU2434233.1"
                     /translation="SFFRLSHRPSWRYLGIGEEEARAFSREVEAAWKEFAEDDCCCID
                     VERKRTFTMMIREGVAMHAFNGELFVQATWDTRPSRLFRTQFRMVSPKRISNPNNTSD
                     SRNCRAGVQINDSGAALGYYVSEDGYPGWMPQKWTWIPRELPGGRASFIHVFEPVEDG
                     QTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKAMYAATIESELDTQSAMDFILGANSQ
                     EQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLMPGDSLNLQTAQDTDNGYSVFEQSLL
                     RYIAAGLGVSYEQLSRNYAQMSYSTARASANESWAYFMGRRKFVASRQASQMFLCWLE
                     EAIVRRVVTLPSKARFSFQEARSAWGNCDWIGSGRMAIDGLKEVQEAVMLIEAGLSTY
                     EKECAKRGDDYQEIFAQQVRETMERRAAGLKPPAWAAAAFESGLRQSTEEEKSDSRAA
                     "
BASE COUNT          768 a         1208 c         1061 g          842 t
ORIGIN      
        1 ttccccagca ccatcgtgtt gtccggcagg aagttctttt tgacgccgtt ttccacgtac
       61 tgtccggaat acacgacgat ggccacatcg ccatacattc ccttataaga caccgctttg
      121 cccaggtctt ttaccgctgt ctccagttcg gaatgagagc cgcgacgggt atccagcttc
      181 tccctgacgg ctttgaagga acggaacagc gcccagcctt tcgggtcaaa cacgatgata
      241 ttcaccacgc cgctggcgtt cagcgcgtag gcttcgatat cgtcggtcgg gtcatacgtg
      301 gacttgtcac gcttgctcca ctccgtgccg ccggactgcg tgatgttgtt cgccgcactg
      361 cggcccatat ccacctcaac cggatcgaag gcttcaccgg tcatggtgta tttgcccttg
      421 agcacggcag aaactgcctg catctcttcg acctgagcaa tggccagctc ttcgtcacgc
      481 atgttctgca tgatgatgcg acggcggcgg taagccgggt ccgccagatt ctgcggatct
      541 tcatccggca ggcgacgcag ggtcatctgc ggattcactt catgcttcgg cttgacatat
      601 cccggcgtaa attcagaggt ggagccgcca cgggaacgga taacctcacc agaaacaatc
      661 ggcgaaacgt acagcgccat gtttaccagt cccggaattt gtgagagata gactttctcc
      721 gtggtgaagg gatagctctc acggaaaaag agacgcagaa acagcggatc aaacttaaat
      781 ttctgctcat ttgccgccag cagttgggcg gttgtgtaca tcgacataaa aaaatcccgt
      841 aaaaaaagcc gcacaggcgg cctttagtga tgaagggtca ggttaaacga tgctgattgc
      901 cgttccggca aacgcggtcc gttttttcgt ctcgtcgctg gcagcctccg gccagagcac
      961 atcctcataa cggaacgtgc cggacttgta gaacgtcagc gtggtgctgg tctggtcagc
     1021 agcaaccgca agaatgccaa cggcagcacc gtcggtggtg ccatcccacg caaccagctt
     1081 acggctggag gtgtccagca tcagcggggt cattgcaggc gctttcgcac tcaatccgcc
     1141 gggcgcggtt gcggtatgag ccgggtcact gttgcccagc ggctggtaat gggtaaaggt
     1201 ttctttgctc gtcataaaca tcccttacac tggtgtgttc agcaaatcgt taacggcatc
     1261 agatgccggg ttacctgcag ccagcggtgc cggtgccccc tgcatcagac gatccagcgc
     1321 agtatcactg cgcgcctgtg cactctgtgg tgctgcggcc agaatgcggc gggccgtttc
     1381 cacggtcata ccgggggttt ctgccagcac gcgggcctgt tcttcgcgtc cgtgagcctc
     1441 ctcacagttg aggatcccca taatgcggct gttttctgcc gcaaccgcag cggtgatctg
     1501 cgcgttcacg tccggctgcg ccgcgctggc gttttcgccc tccgtcgctg gcaccacgcc
     1561 agtaacgtca gcctgcgaag cagtggctga aacagttgtt gattgagtct ctttggtcat
     1621 tcgccctcct gagagacggg atttacgtgc atccagtgca tcacgcataa cggtgatcgc
     1681 atcggtgctg ttgacaagtt catcagccag tccggcatca atggcctcct gaccgctgta
     1741 cactgcagcc tcggtatcca gcacagcctg cacggacagg ccggtatatg ccgacacctt
     1801 ctgcgcaaac atccggcggg ttgcatccat ccgggactgc agtgtttccc ggacatcacc
     1861 cggtagatgg ctgtaggggt tgccatccac cttatggctg ccgctgtaaa tcagcgtgat
     1921 ttccacgccc tgtttctcca gggcagcacc gtaattactg tgagccatca tgacgccgat
     1981 ggagcctgtc cgggcggtct gcgtgaccag acgccgggag gcggcgctgg caagcagctg
     2041 acctgcactg cagttcatgt cgttggccag cgcccatacc ggttttatgt ctcgcacacg
     2101 ggcgatgatg tcagcacagt caaatgctcc cgccaccatc ccgcccggtg tgtccatatc
     2161 gagcagaatg ccgtccacca tcggatcgct ggcagcctgt tgcagacggg cgataatgcc
     2221 gttgtaaccg gtcattcccg aatacggctg cagcgcccgc gtccggctga ccagcgtacc
     2281 ggacaccggc agcacggcga tgccgttcat gacctgataa ctccgggcct gtcgtggtcc
     2341 gtcatcatca ccggataacg ccagcgccgc gggtgcctct ccggcagtca ggctgtcgcc
     2401 ggatactgca tccgtcaggc ggctgatccc aagctggcct gcaagcgcac aaaagaaaac
     2461 ccgcgcatag gcgggttcaa gcatcagcgg ctcattaaaa gccatgctgg caatatgcgg
     2521 gagattacgc agctctgctg tcactcttct cctcctctgt tgattgtcgc agcccggatt
     2581 caaatgccgc agccgcccag gcgggcggtt taagaccagc tgcgcggcgc tccatcgttt
     2641 cacggacctg ctgggcaaaa atttcctgat agtcgtcacc gcgtttcgcg cactctttct
     2701 cgtaggtgct cagtccggct tctatcagca tcaccgcttc ctgaacttct ttcagaccat
     2761 cgatggccat acgaccggag cctatccagt cgcagttccc ccaggcactg cgggcttcct
     2821 gaaagctgaa gcgcgctttt gaaggtaacg tcaccacgcg gcgaacgatg gcctcttcca
     2881 gccagcacag aaacatctgg ctcgcctgac gggatgcgac gaattttcgc cgccccataa
     2941 agtacgccca cgactcgttc gcactggccc gtgccgtgga gtagctcatc tgggcgtaat
     3001 tccgggaaag ctgctcatac gagacaccca gcccggcagc gatataccgc agcagtgact
     3061 gctcaaacac ggagtagccg ttatccgtgt cctgagccgt ctgcaggttc agtgagtccc
     3121 ccggcatcag gtgcggcact tttgcgcctc ccagacggac cggtgctgcg gcgtaatacg
     3181 cggcaatttc accaatccag ccggtcagcc tttcccgctg ctcctgactg ttcgcgccca
     3241 gaataaaatc catcgctgac tgcgtatcca gctcactctc aatggtggcg gcatacatcg
     3301 ccttcacaat ggcgctctgc agctgcgtgt tctgcagcgt gtcgagcatc ttcatctgct
     3361 ccatcacgct gtaaaacaca tttgcaccgc gggtctgccc gtcctccacg ggttcaaaga
     3421 cgtgaatgaa cgaagcacga ccgccgggta actcgcgggg tatccatgtc catttctgcg
     3481 gcatccagcc aggatacccg tcctcgctga cgtaatatcc cagcgccgca ccgctgtcat
     3541 taatctgcac accggcacgg cagttccggc tgtcgctggt attgttcggg ttgctgatgc
     3601 gcttcgggct gaccatccgg aactgtgtcc ggaacagtcg cgagggacgg gtatcccagg
     3661 tggcctgaac gaacagttca ccgttaaagg cgtgcatggc cacaccttcc cgaatcatca
     3721 tggtaaacgt gcgttttcgc tcaacgtcaa tgcagcaaca gtcatcttcg gcaaactctt
     3781 tccatgccgc ttcaacctcg cgggaaaagg cacgggcttc ttcctccccg atgcccagat
     3841 agcgccagct tgggcgatga ctgagccgga aaaaagacc
//
LOCUS       DBFIFM010000289         3871 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.290, whole genome shotgun sequence.
ACCESSION   DBFIFM010000289 DBFIFM010000000
VERSION     DBFIFM010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.290"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(159..854)
                     /locus_tag="N7C74_004519"
                     /pseudo
     CDS             complement(159..854)
                     /locus_tag="N7C74_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(977..1957)
                     /gene="nanS"
                     /locus_tag="N7C74_004520"
     CDS             complement(977..1957)
                     /gene="nanS"
                     /locus_tag="N7C74_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU2434234.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQSYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALVKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNILANIIFVDFQQQGARGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSSAARRGIISDRFVEAILQFWRER"
     gene            complement(2022..3128)
                     /gene="nanM"
                     /locus_tag="N7C74_004521"
     CDS             complement(2022..3128)
                     /gene="nanM"
                     /locus_tag="N7C74_004521"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HGU2434235.1"
                     /translation="MNKTITALAILMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLETQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLISHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAVDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNRLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            complement(3148..3864)
                     /gene="nanC"
                     /locus_tag="N7C74_004522"
     CDS             complement(3148..3864)
                     /gene="nanC"
                     /locus_tag="N7C74_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HGU2434236.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
BASE COUNT          974 a          930 c          891 g         1076 t
ORIGIN      
        1 gtaaaaaata gtaaggtgat cacctgaata aaggtaatct catattttga tgagtatagt
       61 gattttttat ttctctggtg aggaatgaag gaaatatcaa tggtaaggaa cgtcagagaa
      121 tcagggaaaa ttaaggtccc tcagaaacac aatgccgttc acttaagcgg ggcggcactg
      181 tgtttcacag gataacgggt ttttgatatc ttaaccgccc tcgcccttga tggtcggggg
      241 cgttttgtta tgaacaccac ttccagagcc cccgcagatg ctccagtcgt ttcgggatag
      301 tccccggtga cgccgtgttt cccagcacta tcatttctgt cacgataaac tggaacgcga
      361 acttaaagct gatctctgac gccgcttttg ttgcctcacg ccgtatcgct ccgccggtag
      421 gcgcggtcat ggcattcagt agaatgtgat tcccagattc attaacgcca ccagacgcat
      481 gacggggtag gcgctctgcc gctcagtgga tgtgttggca gagccataat attcacgcag
      541 ctcgggttca tcaggtgtcc tgaactgtgc gccatcaatg gcaaaaagtt gcaggccgtg
      601 ccagtcatcc ttcaggtaac gttccgcgcc ccatgtctgt gtggtctggc ggaagagcca
      661 ttccaccggg gcggccccca cgcgctgacg cgcctgggtg acagcgctgc gggcgccagc
      721 aggttcatcc ccgcttcgcc atccacgctc aggttaagac gacgaacggt cgcatgagca
      781 gaaagcgtca ggaagtgctg tatccactcg gtgggaaggt gctctgcaaa cagttgcgta
      841 gagggtggtg gcataagcgg atggtcactg aaagtggtat aagaaaatgg ctccttgttg
      901 tggaagtcgt tatagtgccg cacgccggag gagttaaaag cgctgatgga tgcgaataaa
      961 tcaaattaat gaacgctcac ctttcgcgcc aaaactgcaa aattgcttct acaaacctgt
     1021 cagaaataat cccccgacgg gctgctgagc tgaaatgact gcttcgcagt gccgtcgtcc
     1081 agttctccgg tgaccggtac gctgaaccgt aatatcccgt gcttaaatcg tccggatctt
     1141 catcaggcgc gttcgtcagc cctcttgcac cttgttgctg gaagtcgacg aaaataatat
     1201 tagctaaaat attattttga taattgccat aaatagcttc atacgcatga gggaaatttt
     1261 ctttccagta ccaggtggta tcgccgcaaa accacggcgc gtcagtaata ttattaagct
     1321 gagaatggta ttgttttaga tccctacgaa aggcttcaac catatgatta aagtgttgag
     1381 gatgtgacgc gtagtcactg gtcattaagt caaattcgcc ttgcatccag catacgccga
     1441 ggaatttgtt ctgcggattt tttaccagtg cggctcgcgt tctgctgact aaatcctggt
     1501 atagcggagt atccgttccc caacgacaag catcatggct ggctccgtgc cgttctgaat
     1561 atgtcccctc gctgcctgcg gtaaaagccg atccgccacg gcaacacgga acgatgagaa
     1621 tccctgcatt atcaggaatg aagggcagta atttccgtgc aatatgcagt gcctggccaa
     1681 cggtgccgta ctgtgtttga tgattcgttg ccagaggatg gtgataactc tgcatatcct
     1741 gaacatcgtg tgggcagtga gtcagtggaa taatgtcgtt aaagtgacat gacgggcctc
     1801 cgggatgcgt atgcgcaaat ctcgctaatt gtttaattct gggatgaggc gcatcttccc
     1861 tgtccggtaa tggcagtcct tcgccatacg ccatggcatt agactgacca gcaacagtaa
     1921 gaacatagta ataatcaggc gatattattg cgttcatata aatttcgcgt aattgaaatg
     1981 acaaattgat agcagggccc ctttaccggg gccctgaagc gttagttttg tactgtgact
     2041 ttattatcct tcacagagat caaaactgaa tccgtcaccg ctttgccgcc tgcagtttca
     2101 ccgccaataa tcaatagact attattccag ggcaatgata ctccgtaggc ccgaccttgc
     2161 gataattcac ccgatttatc ccatttcccg ttatgccaaa gatgaatatc agtgctatat
     2221 gattttttca ggccttcatg cgcatagttc ttaccgtttt ggtaattttc tcgtgaacct
     2281 ttgaatccgg cccctccggc aaatataaga gaatcattgc ttatccccgc aaaaccgcca
     2341 gcgacgccat ctggtgatga gacgggagca agcctattcc attttaaatt attaccggtg
     2401 aaatcaagtt caaatacggc atccgttcgc aatccgggtt tggcttcgcc attaataagc
     2461 caggttttat cacctttatt cacaaccgcc gcaccagccg tcccgtacca gggagattcg
     2521 ccagcgtaac tccattgctg tgtagaggga tcaaaagaca acagaaactt attaaagaaa
     2581 taatcttctg cttttttgtc aaaatagtgt gcattgattt tatctacagc ggttgaatct
     2641 tttccagctt cgttgagatc ttcaaaatag ccattgaaga tattctggtt aacaccgcca
     2701 gtaacataag ccttgccgtt gtgtacaaaa gtcacatggc ccgccatgcc catcggcgcg
     2761 tgcgatatca atttaaccca gctattggtt ttggggttgt atttgtgtac gtcattaaat
     2821 acctgagtca agccctcgct gtttttgcca atgccgccaa acacatacag attgccatca
     2881 ataaatgccg aggttgcttg atctcttggt ccaccaggga atgcagctaa cgctgtccat
     2941 tttttatctt tggcctgtgt ctccagcttg taccatgccg tacctgcgct acctaaacca
     3001 atgtagacag tgtcgttatc aattgctccg gtaccacttt taaatggtac aggagtttcc
     3061 ggtaatacag acgcgtttgc ggcaaatgaa gccattagga tagcaagcgc cgttattgtt
     3121 ttattcattg tgactgtctc ctgtctacta cagtttaaat gacacaccaa tgcgataact
     3181 gttttccgat aaattatctc tgccgttgta aacaccctga cggtccaggt agtcatattc
     3241 tatgtatggc gtaatatcgg gcgtcatatg gtattgtaga acaaatgcat tttccgtcgc
     3301 ccatttctta tggtttgcat agcgataatc gttctgtttg ctgtatagcg tcgtttgcca
     3361 tgcgaaggtg aaatcactat taatatggta agtgacatat ccatcccaac gatgaacgtt
     3421 atcacgagac atatcaccgg ataagtcttg ttgtcggtaa gctttccagt cgtaacgata
     3481 gcgaatgcca aaattaagat cttttgtcgc gtcccaggac agttttacgt agggtccgta
     3541 gcgtgtgccg ttgctgctaa aatgcgttaa cattcccggg cgcaccgtcc attgatcatc
     3601 aagtttaatc gcgtaattaa cttcaacctg aacatcattg agtgcggcat tttccttttt
     3661 attatcatga atggtattcc aggtattact ttccatgctt gcccaccatc cattttgcca
     3721 tccctcactg actttgagtc gagtctcata ggcgtggctt ccactacgat atccaccacg
     3781 tacgtccagt gtcgcagcct gagaaattaa tggtgacgaa aagcacagta ataatacgcc
     3841 agaaagtatt ttagcctttt tcataaattt c
//
LOCUS       DBFIFM010000290         3863 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.291, whole genome shotgun sequence.
ACCESSION   DBFIFM010000290 DBFIFM010000000
VERSION     DBFIFM010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.291"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            82..309
                     /locus_tag="N7C74_004523"
     CDS             82..309
                     /locus_tag="N7C74_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434237.1"
                     /translation="MACNVFAFSNSGGGSETSYKEPEKTSAMTTTHSTKFQPSQAILF
                     KMREDAPPLNLTEETTPPFPTKANYLIHPVR"
     gene            complement(386..628)
                     /gene="yfdY"
                     /locus_tag="N7C74_004524"
     CDS             complement(386..628)
                     /gene="yfdY"
                     /locus_tag="N7C74_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HGU2434238.1"
                     /translation="MFYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            981..1901
                     /gene="lpxP"
                     /locus_tag="N7C74_004525"
     CDS             981..1901
                     /gene="lpxP"
                     /locus_tag="N7C74_004525"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HGU2434239.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2257..2328
                     /gene="ypdK"
                     /locus_tag="N7C74_004526"
     CDS             2257..2328
                     /gene="ypdK"
                     /locus_tag="N7C74_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HGU2434240.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2393..3631)
                     /gene="alaC"
                     /locus_tag="N7C74_004527"
     CDS             complement(2393..3631)
                     /gene="alaC"
                     /locus_tag="N7C74_004527"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HGU2434241.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAILESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT         1003 a          972 c          893 g          995 t
ORIGIN      
        1 attctcagtg aaccattttc aggataactt tctatgaaag taaacttaat actttttagc
       61 ttatttttat tggtctctat tatggcatgc aatgtttttg cattttctaa ttctggtggt
      121 ggtagtgaga cgagctataa agagccagaa aaaacgtcag cgatgacgac aactcactct
      181 acaaaatttc agccatcaca ggcgattttg tttaagatga gagaagatgc accaccatta
      241 aacctcacag aagaaaccac gccccctttt ccgacaaagg cgaattatct tattcatcct
      301 gtgcgatagc gtctgtcacc tggcaggtga acaatcatgt ctgatgctga tgtgaacacc
      361 tgcccaaatg aaccatcctg acccactatc tcctgccata ccccaccata aagcgaatca
      421 tcaagccaca agccggggca agaaacataa atatccccgt caccagttca ttaccgccag
      481 ttaaccacat ggtgaagtaa taaatgagtg cagcgaggat caccatgccg aagaaagaag
      541 agaccgccga taccacgttc agtacctgac cgatgtatcc gcttacgcag acaatacaca
      601 gggcgagaaa catccataaa taaaacatag ctcaacctcc tgccgcgcta aaaaacctgt
      661 aacttcagtc actttaatag taagacaatc cttaacctct ggcaattttt gctaagcgtc
      721 aatgaaaaca gcatgttaaa tgcaatacta ttgcgtgcgg aaaatttttt actttgcacg
      781 attaataatc agtagctgaa agcagtcagc cagtgataaa gtacagatac cgcattcatg
      841 ctcctcgtgg ttatgtcctg actagtcttt acactcttta caggaaccat tgtcgtacat
      901 gatggcccaa ccaattgagt gtaccgctgt cgacatggaa tcttctgcga tacaacaatt
      961 cgtatctaca gaaggtaact atgtttccac aatgcaaatt ttcccgcgag tttctacatc
     1021 ctcgctactg gctcacatgg tttgggcttg gtgtactctg gctttgggta cagcttcctt
     1081 atcctgttct ctgctttctc ggcacgcgta ttggcgcaat ggcgcgacca ttcctgaaac
     1141 ggcgtgaatc tatcgcccgt aaaaacctgg aactttgctt cccgcagcat tctgcggaag
     1201 aacgcgagaa gatgattgcc gaaaactttc gttcactcgg catggctctg gtagaaaccg
     1261 gcatggcgtg gttctggccc gacagtcgcg tacgtaaatg gtttgatgtt gaagggctgg
     1321 ataaccttaa acgcgcacaa atgcaaaatc gcggcgtaat ggttgtcggc gtccatttta
     1381 tgtcgctgga actgggcggc cgcgtgatgg gactttgtca gccaatgatg gctacctatc
     1441 gtccacataa taatcagctg atggaatggg tgcagacccg tgggcgcatg cgctctaaca
     1501 aagcgatgat cggcagaaat aatctgcgcg gcattgtcgg tgcactgaag aaaggtgaag
     1561 cggtatggtt tgctcccgat caggattatg gtcgtaaagg cagctccttc gcgccgttct
     1621 ttgcggtgga aaatgtcgcc acaaccaatg gcacctatgt tctctcccgt ctctctggcg
     1681 cagccatgtt gaccgtaacg atggtaagaa aagcggatta cagcggatat cgtttgttca
     1741 tcaccccaga gatggaaggc tacccgacag atgaaaatca agccgctgcc tatatgaaca
     1801 agattatcga gaaagagatc atgcgcgcac cggagcagta cctctggatc caccgtcgct
     1861 ttaaaacgcg cccggtggga gaatcgtcgt tgtacattta acaaactgct cttactcttt
     1921 ccctcctcaa atcggccata ataacctcat ggtttttatg gcttttttct taagattttt
     1981 tccttcgctg aatatctaat tattaccgcc agaaattaaa ctgtcgccga tatgagacac
     2041 ctgtaagtta ctaaaagtaa ataagaaaat gcctcttgtc tcgtttcagt tttaggcgta
     2101 cattagcgcc gtctcgagca gtttagctca gaattctggg gtgatgccca cggtaaaata
     2161 caggactttt aaggtcgttt tgcccggtgt caactcactg tatcaggtgt gatgaagtca
     2221 ttcaggcgta accgtaatta cgcggagaga tgtaaagtga aatatttctt tatgggcatt
     2281 tcttttatgg tcatcgtttg ggccggtact tttgccctga tgatctaaaa gcaaactgtg
     2341 caatgaaaaa caggagccat caggctcctg tttgcatttc tgacgacggt gtttattccg
     2401 cgttttcgtg aatatgtttg ctgctggcgg gtagtaaacc gtcggcacgg aacatcgctt
     2461 taatcccacg aatcgcctga cgaatacggt cgcggttttc aatcagtgca aagcgaacat
     2521 gcgtgtcgcc gtagtcacca aagccaatcc ctggagagac acagaccttc gcttcgttaa
     2581 ggagcttctt ggcaaattcc agcgatccca tggccgcata tggttccggg attttcgccc
     2641 agacatacat cgaagccttc ggcatttcga ccatccagcc cgcttcatgc agccctttaa
     2701 ccaatacatc gcggcggcgt ttgtactgtt cggcaatgtc gcgcacgcac tgttgatcgc
     2761 cctccagcgc cgcaatcgcc gccacctgca acggcgtaaa agtgccgtaa tcgtgatagc
     2821 ttttgattcg cgccagggca ctgaccagcg ttttgttgcc gaccataaag ccgatacgcc
     2881 agcccgccat gttgtagctt ttcgacagcg taaagaactc gactgccaca tcgcgtgcac
     2941 ccggtacctg catgattgac ggcgctttcc agccatcgta gacgatatcg gcataggcca
     3001 ggtcatggac caccagcaca tcgtagcgtt tcgccagcgc cactactttt tcgaagaact
     3061 ccagttccac gcattgcgcg gttgggtttg acgggaagcc gaggatcatc atcttcggtt
     3121 tcggataact ttcaagaatg gcgcgctcca gttcgttgaa gaaatcgaca ccttccacca
     3181 gcggcactga gcgtacctgc gccccggcaa tcaccgcgcc ataaatatgg atcgggtagc
     3241 ttggattcgg caccagcacc gtgtcaccat gatccagcgt cgccagcatc aggtgtgcca
     3301 gcccttcttt cgaaccaatg gtgacgatgg cttctgattc cggatcgatt tcaacgtcgt
     3361 agcgatcctg ataccagcgg gaaatggcgc gacgtaaccg cggaatgccg cgtgaagtgg
     3421 agtaaccatg cgtgtccgga cgctgggcca cagtacataa tttttcgacg atatgcggcg
     3481 gagtcgcacc gtccgggtta cccatgctga aatcgataat atcttcgccg cgccgacgcg
     3541 cagccatttt cagttcagcg gtgatgttaa aaacgtaggg cgggagacga tcaatgcgcg
     3601 taaagcgacg ttcagggcga gtgtcagcca taatttcctc agattaacgt tagcgcccgg
     3661 accgtccgag cgacgctgcc acgatggtgg ctgttttgaa aatagcctga ttaatttctg
     3721 tctgtccagc gttttagaac atttttatta atgacgttaa atgaggaact ctgctttaaa
     3781 aacaggaaat ttatctgcgc taacaagaaa attaccactc attttattaa cataataatt
     3841 tcaataaatt tactaaccag aag
//
LOCUS       DBFIFM010000291         3778 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.292, whole genome shotgun sequence.
ACCESSION   DBFIFM010000291 DBFIFM010000000
VERSION     DBFIFM010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.292"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(90..758)
                     /gene="artM"
                     /locus_tag="N7C74_004528"
     CDS             complement(90..758)
                     /gene="artM"
                     /locus_tag="N7C74_004528"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HGU2434242.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(758..1474)
                     /gene="artQ"
                     /locus_tag="N7C74_004529"
     CDS             complement(758..1474)
                     /gene="artQ"
                     /locus_tag="N7C74_004529"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HGU2434243.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGIVEIPVQMQIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPMGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1481..2212)
                     /gene="artI"
                     /locus_tag="N7C74_004530"
     CDS             complement(1481..2212)
                     /gene="artI"
                     /locus_tag="N7C74_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HGU2434244.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITAEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2230..2958)
                     /gene="artP"
                     /locus_tag="N7C74_004531"
     CDS             complement(2230..2958)
                     /gene="artP"
                     /locus_tag="N7C74_004531"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HGU2434245.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3176..3691)
                     /locus_tag="N7C74_004532"
     CDS             complement(3176..3691)
                     /locus_tag="N7C74_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU2434246.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYHIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
BASE COUNT          947 a         1001 c          948 g          882 t
ORIGIN      
        1 atccggcaca ggcaccgtgc tgatgtctga tgcgacgctg gcgcgtctta tcagacctac
       61 aaaacccccg gcgaatgtac gcagccacat taattccttc gctcaaacgc cagcgctttg
      121 cgctcgatca gacgcatcat cagcgtcagc aggccgttaa cgaccagata aataatccct
      181 gccgcgccga acaccatgac atcgtaggtg cgtccgtaca acaactggct gtatcccatc
      241 acttccatca gcgtaatggt gtaagccaga gaggtacttt tgaacaccag caccacttcg
      301 ttggaataag aagagagcga gcgtttaaag gcatacggca gcaggatcgc cagcgtatct
      361 tttttgctca ttcccagggc gctacaggac tgccactgac cttccgggat cgcacgaatt
      421 gcaccgtaaa acagctgcgt ggtatacgcc gcactgttca gcgacaacgc aatcagcgca
      481 cataaccacg gttctgacaa caaatgccac agtgccggat actcctgcaa cgtcgggaac
      541 tgacccggcc cgtaataaat caggaagatc tgcaccagca gcggtgtacc ggtaaacagc
      601 gtgatataac cccgcaccag ccacaccagc accggcgttt tcagcgtcag gatgatggta
      661 aaaatcaacg ccagaatcag tgccacaatc agcgaggcaa cggttagcgt caggctggtg
      721 tgcagccctt tcatcagttc gggtaaatac tcaaacatta gctgggcctc cgctcaaaac
      781 gtgtcgcgcg caggtcaatg cgtttgagaa tgtactgact gagcagggtg atcaccaggt
      841 aaatcgccgc cgccacaatg taccaggtaa atggttcctg ggtacgagta gcgatgcttt
      901 tggtttgcag cattaaatca ttcacactaa tcaaactgac cagcgcggta tctttcagca
      961 gcaccagcca ctggttaccg aggccaggca gcgcatgacg ccacatctgc ggcatcacca
     1021 gacggaaaaa gatagccgat ttcgacagcc ccagcgcctg gccggattcc cactgaccca
     1081 tcggcaccgc tttcaacgcg ccgcgcagcg tttgcgaggc ataggcggca tacagcagtg
     1141 acagagcgat gacaccacaa aggaacgggc taacgtcgaa gttttcaatc tgcatctgca
     1201 ccgggatctc tacaatccca agattgatag tgaagccatc cgaaagtgtc agcagcagct
     1261 gcgaggagcc aaaatagata aacagcacca ccagaatttc tggcaggcca cgcagaatgg
     1321 ttaccagcgc tgaacctgcc cacgcgacag gacgccattt cgcagactcc cataccgcaa
     1381 agaacatcgc cagcgccagc ccgacaatca atgcacaaac ggcaaggccg acggtcatcc
     1441 cggcggcgct tgctaaagga aaaaattcat tcatcaggaa ttacttctgg aaccatttgt
     1501 tgtagatggt ttcgtaagtg ccatctttct tcactttttc cagcgcagtg ttgagtttct
     1561 gctgcagctc agtgttgccc tgacgtaccg cgatgccgag gccagtaccg aagtaatctt
     1621 tatcggtcac tttatcgccc actgccgcca gtttcgggtt atctttcagc cattcagtta
     1681 ccactgcggt gtcaccaaat acgctgtcga tacgaccgtt ttgcagatcc agttttgcgt
     1741 tctggtagct gtcatacggc acggtggtga tttccgggtg cttatccata atgaatttct
     1801 ggtgagtcgt accattctgt acgccgactt ttttgccttt cagctgatca acgctggtgt
     1861 atttaccctg ctgacccaca aacagggcag agttgtcata gtacggcgtg gtaaacagta
     1921 cctgcttttc acgttccgcg gtgatatcca tcccggccat caccgcttcc acgcgacgga
     1981 atttcaggct tgggatcagg ctgtcaaatg cctggttaga gaaagtacag gtcgcatcaa
     2041 tctctttaca cagcgcctgt gccaggtcga cgtcaaaacc aacgatctgg ttgtttgcat
     2101 caatcgattc aaacggagga taggaggctt cggtagcaaa acgaatggtt tcggcagctg
     2161 tggcggaaag actaaaacct gcaattaacg cggcaatcag aacttttttc attgttgtta
     2221 tcccgaatct tagtgagaga gatagttttt aaatgcttcg gtttgcggct cggtaaagca
     2281 gctcgcgtcg ccttgttcta cgatatgacc gttttccata tacaccactc ggctggcggt
     2341 tttacgcgcc acttcaactt cgtgggtgac gatcacctgg gtaatattcg tttctgccag
     2401 ctcacgaatg atgctgacga tttgtgccgt aatttccggg tccagtgcgg cggtcggttc
     2461 atcaaacagc agtacctgcg gttccatcat taacgcacgg gcaatagcaa cacgctgctg
     2521 ctgaccacca gaaagatgca gcgggtaacg atcgctataa ggtttgagac gcagacgttc
     2581 cagcagtttt tctgcacggg ccagcgcctg atctttactc aaccccagta cacggcaggg
     2641 cgcttcaatc aggttttgct gcacggtcag atgcggccac aggttgtatt gctgaaacac
     2701 catgccaacg ttacgacgca aatcgcgaat cgctttgtct gagggtgttt tggtgaaatc
     2761 gaaatggttg cctgcaatgt tgagcgtacc ggagcgcggc atctcaagca gattgagtac
     2821 acgcagcagc gagcttttac ccgcgccgct ggggccaagt aacaccagcg tttcgccctg
     2881 tgggcaatcc agcgtgatat cgaacagcgc ctgatgcgcg ccgtagaagc aattaatgcc
     2941 gtttaattga atactcattg acactcgtat actggcagtc tgatagctat tgaggtcgaa
     3001 gatagtacct ttgacagaat aattatgcaa tatttctgct ttaaaagtta aaagcaaagc
     3061 gcattattca ataaacatag cacaaaataa cgggggcggt ggtcggcgag cataaatgtc
     3121 ggcattcctc acgaaatgcc ggacaattta cggggtttat tggttgatca aggcgttagc
     3181 gattctcgat ggactgacgg agcgtacccg ccgtggcatg aacgctaccg cctaagtagc
     3241 gcacatcgtc gatcacccaa cactggcctt cctgaatcat taacacttca tcctgccagc
     3301 cctggtcacc ctgcttgaga tccacgcgca atggaatgtt acgggcatca cgattaggga
     3361 tagtcgatgc actggcaacg tgggcgctgt ctggcaaggt ggttcgactg gagaacggat
     3421 cactgcttaa caattcacga tgactattat cgcggctggc atcgctaagt agggttgcca
     3481 gtttgtcact caaatagggg cgcagggcgg taatgtcatt gctgcgatgt tgaatgtggt
     3541 agtcatagaa ttgttgggcc acgctgtcag gccccccctg gacacaggaa ccggtgcggg
     3601 gaccattatc tttataagct ggcgtgacgg tggtacaggc actcagcagc agtgcacatg
     3661 gaataagtag agatagtgaa cggtagcgca taatcatttc cttattagct tgctgtctgc
     3721 gtcacactac gaccaaacgc tatcagatta tttaatacat tgtttttaat acaattct
//
LOCUS       DBFIFM010000292         3777 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.293, whole genome shotgun sequence.
ACCESSION   DBFIFM010000292 DBFIFM010000000
VERSION     DBFIFM010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.293"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(16..2349)
                     /locus_tag="N7C74_004533"
     CDS             complement(16..2349)
                     /locus_tag="N7C74_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HGU2434247.1"
                     /translation="MSGMKVSQAEKAARGHWSRILPALGVNVLKNRHQPCPVCAGKDR
                     FRFDDQEGRGTWFCNQCGAGDGLALVSKVLDVGISEAADRINGIIGNLLPVSQGMLES
                     GSPEKEDGRKAAAVLAARLFDKSRQTTGNAYLTSKGFPALPCRELTAMHKVGGVAFRA
                     GDLVVPLYADGELVNLQLINANGGKCFLKGGQVKNAFYLVEGTAKAAKRLWIAEGYAT
                     ALTINYLTGDAVMVAFSSVNFLSLASIACSEYPTHQIIIAADRDLNGAGQTRGAAVTG
                     ACNCTMALPPVFGDWNDAFTQNGEEATRHAIHEVIKPAVASPFDTMSEAEFTALSVSE
                     KAQRVVDHYKNSLAVDPNGQLLSRYEAGAWKVIYYADFARDVAALFQRLDAPFSSAKI
                     ASLVETLKLIVPQQQNPARQLIGFRNGVLDTRTGLFSPHDKKHWLRTLCEVDYTQPVD
                     GESLETHAPAFWRWLDRAAGFNPEKRDIILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     ILAEIATMLAGEDNATSATIEMLESPRERAALIGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYQNAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVILHFPDQIAPEERD
                     TQLKEKIASELAVIVRQLMQRFSDPMSARTLLQSQQNSDEALTIKRDADSAFDFCGYL
                     EVLPDTTGMFMGNANIVPRQPRTYLYHAYLVYMEANGYKNTLSLTMFGKGLPLMLKEY
                     GLQYEKRRTNQGMQTNLALREESNADWLPKCDEFAAK"
     gene            complement(2363..2686)
                     /locus_tag="N7C74_004534"
     CDS             complement(2363..2686)
                     /locus_tag="N7C74_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 family protein"
                     /protein_id="HGU2434248.1"
                     /translation="MICCESLLALRAALYRRAVACAWLALSNHQERYSGLTLAELEDA
                     IARELEGFYLRQHGQQRGLEIACALLSDLMESGPLKACPVLSLLGMTVMDELCSRHLN
                     KPALH"
     gene            complement(2686..2907)
                     /locus_tag="N7C74_004535"
     CDS             complement(2686..2907)
                     /locus_tag="N7C74_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434249.1"
                     /translation="MNQFEISYDDVVRLKHLRNVGEYVTGMAALQDCYEKPAGAQCEQ
                     LVSLIYLMTEQLDGVVQRCHDDLMNAEVA"
     gene            complement(2904..3461)
                     /locus_tag="N7C74_004536"
     CDS             complement(2904..3461)
                     /locus_tag="N7C74_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU2434250.1"
                     /translation="MMIPAYIKYLYSGLLTVVISRYSFSAVAKSAAGIGVPYNLLATI
                     DAPCVFFYVVAQAQPFSGLWCLCLHHGSIEIMVVRAGQPSGWPVSNKAGYANPVRAAT
                     SEIGVSGGSNNRYLLEAAIMATILTPSHPQYVFVFAAIRRADTHPRICMLRTVSCDER
                     SARRLLVRDYVLSLSARLPAGEVTL"
     gene            complement(3458..3718)
                     /locus_tag="N7C74_004537"
     CDS             complement(3458..3718)
                     /locus_tag="N7C74_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2434251.1"
                     /translation="MRNISVSAPAPAAPLFPVSNHHERFLRLPEVMHLCGLSRSTVYD
                     LISRDAFPKQIPLGGKNVAWAQSEVSAWMADRISARGRGCDA"
BASE COUNT          912 a         1035 c          957 g          873 t
ORIGIN      
        1 ccggtcttag gtcagttatt tcgctgcaaa ctcatcgcat tttggcaacc agtcagcatt
       61 gctttcctct cttagggcca gattagtctg cattccttga ttggtccgtc gtttctcata
      121 ctgcagccca tattccttga gcattaacgg tagccccttg ccaaacatgg tcagactgag
      181 cgtattttta tagccgttag cctccatgta gaccagatag gcatggtaga ggtaagttcg
      241 aggctgacgt ggaacaatat tagcgttccc cataaacatg cccgtggtgt caggtaggac
      301 ctcaaggtag ccgcaaaaat caaaagctga atcagcatca cgtttgatgg tgagcgcttc
      361 atcggagttc tgctgtgact gaagcaatgt cctggcactc attgggtcgc tgaaacgctg
      421 cataagctgg cgaacaatca ccgctagctc actggcaatt ttctctttga gctgggtatc
      481 gcgttcctcc ggggctatct ggtcggggaa atgcaggatc acccttcggc gtgaaactcc
      541 accactacga tcagtgaagc gcatcggatt attgttcaca gccaggatga ccgccgggat
      601 gtgggttgaa taggcgttct gatatttggg atcaacggat accgcatcgc cgccagtgat
      661 ggcttttagt ccggccccgt caccgctcca cttttcctgg tcgggaagtc gaatcagtga
      721 aaaacctatt aacgcagctc gttctcttgg cgactcaagc atttcaatgg ttgccgaggt
      781 cgcgttatct tcacccgcca gcatggttgc tatttcagca agaatactct ttccacttcc
      841 gccagggcca gtgacctcca gaaacagctg ccagtcataa cggttagcca gcaccataaa
      901 caatgcagcc agaataatgt cccgtttttc aggattaaaa cctgcggcac gatccagcca
      961 gcgccagaat gccggggcat gggtttccag tgactcaccg tcaacgggct gcgtgtaatc
     1021 cacctcgcac agcgtacgta accagtgctt cttatcgtgc gggctgaaca atcctgtccg
     1081 ggtatcgagc acaccgttgc gaaatccgat aagttgccgc gccggattct gctgttgcgg
     1141 aacgatcagt ttgagggttt ccacgagaga cgcaattttc gcggatgaaa aaggtgcgtc
     1201 gaggcgctga aacagcgcag cgacatcacg ggcaaaatcg gcgtaataga taactttcca
     1261 ggcccccgcc tcatagcgtg aaaggagctg cccgttcggg tctactgcca gtgaattttt
     1321 atagtgatcc actaccctct gcgctttttc gctgacgctc agcgcggtaa attcagcttc
     1381 gctcattgtg tcgaaggggc tggcaacagc tggttttatt acttcatgaa ttgcatgccg
     1441 ggtggcttct tcgccgtttt gcgtgaatgc atcgttccag tcaccaaaca caggcgggag
     1501 cgccattgtg caattgcagg ccccggtaac agctgcgccc cttgtttgcc ccgcaccgtt
     1561 gagatcgcgg tcagcagcaa taattatctg gtgcgttggg tactcactgc aggcaatgct
     1621 cgccagggaa aggaaattga cggacgaaaa ggccaccatg acagcatcgc cagtcagata
     1681 gttgatagta agtgcggtgg catatccttc cgctatccag agccgtttgg ctgctttggc
     1741 agtaccttca accaggtaaa aggcattctt aacctgaccg cctttaagga agcatttgcc
     1801 cccattagcg ttgattaact gcagatttac cagctctcca tctgcataca atggaacgac
     1861 aagatctccc gcgcgaaatg ccacaccacc gactttatgc atagcggtta attcccggca
     1921 aggcagtgca ggaaaccctt tactcgtcag ataggcattg ccagtggtct ggcgggactt
     1981 atcaaacaaa cgggcagcca gcactgctgc agcttttctc ccgtcctctt tttcaggaga
     2041 accagattca agcattccct gagatactgg cagcaggttt ccgataatgc cgtttattct
     2101 gtctgccgct tcactaatgc ctacatccag tactttactt acaagcgcca ggccatcacc
     2161 tgccccgcac tggttacaga accacgttcc ccgcccttcc tggtcatcaa atcgaaagcg
     2221 gtctttcccg gcacagaccg ggcagggctg gtgccgattt ttcagtacat ttacgcccag
     2281 cgcaggtaaa attcttgacc agtgaccccg agctgctttc tcagcctggc taactttcat
     2341 tcctgacatt gtgcggccct ccttagtgca gcgctggttt gttgaggtga cgggaacaaa
     2401 gttcatccat gaccgtcatt ccgagcagtg agagaaccgg acaggcttta agcggccccg
     2461 actccatcag atccgaaagc aacgcgcagg caatttccag ccctctttgc tgcccatgct
     2521 ggcgcagata gaacccttcc agctcgcggg ctatggcatc ttcaagttca gcgagcgtca
     2581 ggccggaata acgttcctga tgattgctta gagccagcca ggcacaggca acggcacggc
     2641 gatacagagc ggcacgaagt gccagaagag attcacagca tatcatcagg ccacctccgc
     2701 attcatcagg tcgtcatggc agcgttgcac cactccatcc agttgctctg tcatcagata
     2761 aatgagggaa accagttgtt cgcattgggc acctgctggc ttttcataac agtcctgcag
     2821 ggctgccatg ccagttacat actcacccac attgcgtaaa tgtttcagcc ttacaacatc
     2881 gtcgtaggaa atctcaaact ggttcatagc gtcacctccc cggcaggcag ccgtgcagaa
     2941 agggagagta cataatctcg gactagcaaa cggcgcgcgg aacgctcatc gcaggagaca
     3001 gtgcgaagca tacagatgcg ggggtgagtg tcagcacggc ggattgcggc aaacacaaag
     3061 acatattgcg ggtgtgacgg ggtgaggatt gtagccatga tggcagcctc caataagtag
     3121 cggttattgc taccaccgga aacgccaatt tcactggtgg cagcccgaac ggggttggcg
     3181 taaccggcct tattggaaac cggccagccc gaaggctgcc ccgcccggac taccattatc
     3241 tcgatagaac catggtgtaa gcataaacac cacagccctg aaaatggttg tgcctgagct
     3301 acgacgtaaa aaaagacgca aggggcgtca attgtcgcca ataagttata cggaacgcca
     3361 attccggctg ccgattttgc gacagcggaa aaactatacc tggaaatgac gacagtaagc
     3421 aagccagaat aaaggtattt aatgtatgca ggtatcatca tgcatcacat ccccgcccac
     3481 gggcgctgat gcggtccgcc atccatgcgc tgacctcaga ctgtgcccag gcgacatttt
     3541 taccgccaag cgggatctgc ttcggaaacg catcgcggct gatcaggtcg taaacggtcg
     3601 aacgggacaa cccgcataag tgcatcacct caggcaggcg taaaaatcgc tcatgatggt
     3661 tcgatacagg aaataatggc gcagcaggag ctggcgcaga cacagaaata ttacgcattt
     3721 atctaccctc tttaatttcc acaacagtcc ggatgaattt atccgggttc aggtagt
//
LOCUS       DBFIFM010000293         3755 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.294, whole genome shotgun sequence.
ACCESSION   DBFIFM010000293 DBFIFM010000000
VERSION     DBFIFM010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.294"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..533
                     /locus_tag="N7C74_004538"
     CDS             <1..533
                     /locus_tag="N7C74_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGU2434252.1"
                     /translation="FMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKDTPSFGHSCRHS
                     MCIEMHRLLSETQTMLAGYYWVMEYTSNKGLHIHFIGYLDGQRHNKSYRISRQLGDIW
                     RRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAKQDQKEHGIILR
                     RSRLPEKSNRGRPRHN"
     gene            688..3048
                     /locus_tag="N7C74_004539"
     CDS             688..3048
                     /locus_tag="N7C74_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HGU2434253.1"
                     /translation="MVHKSDSDELAALRAENARLVSLLEAHGIEWRRKPQTPVQRVSV
                     LSTDEKVALFRRLFRGRDDVWALRWESKTSGKSGYSPACANEWQARICGKPRIKCGDC
                     AHRQLIPVSDLVIYHHLAGTHTVGMYPLLEDDSCYFLAVDFDEAEWQKDASAFMRSCD
                     ELGVPAALEISRSRQGAHVWIFFASRVSAREARRLGTAIISYTCSRTRQLRLGSYDRL
                     FPNQDTMPKGGFGNLIALPLQKRPRELGGSVFVDMNLQPYPDQWAFLVSVTPMNVQDI
                     EPTILRATGSIHPLDVNFINEEDLGTPWEEKKSSGNRLNISIAEPLKITLANQIYFEK
                     AQLPQVLINRLIRLAAFPNPEFYKAQAMRMSVWNKPRVIGCAENYPQHIALPRGCLDS
                     VLSFLRDNNIAAELIDKRFAGTECNAVFMGNLRAEQEEAVSALLRYDTGVLCAPTAFG
                     KTVTAAAVIAKRKVNTLILVHRTELLKQWQERLAVFLQAGDSIGIIGGGKHKPCGNID
                     IAVVQSISRQGEVEPLVRNYGQIIVDECHHIGAVSFSAILKETNARYLLGLTATPIRR
                     DGLHPIIFMYCGAIRHTAVRPKESPHNLEVLIRSRFTSGHLPSDARIQDIFREIALDH
                     DRTVAIAEEAMKAFGQGRKVLVLTERTDHLDEIASVMNSLKLSPFILHGRLSKKKRAM
                     LISGLNALPPDSPRILLSTGRLIGEGFDHPPLDTLILAMPVSWKGTLQQYAGRLHREH
                     TGKSDVRIIDFVDTAYPVLLRMWDKRQRGYKAMGYRIIADGDESVI"
     gene            complement(3210..3386)
                     /locus_tag="N7C74_004540"
     CDS             complement(3210..3386)
                     /locus_tag="N7C74_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434254.1"
                     /translation="MTEILRIAQTYEPWLDFWELVVQNVSTIASHTATNRPLPEAIMA
                     NYQLDEQLLEGCRP"
     gene            complement(3524..3755)
                     /locus_tag="N7C74_004541"
                     /pseudo
     CDS             complement(3524..3755)
                     /locus_tag="N7C74_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          962 a          784 c          969 g         1040 t
ORIGIN      
        1 acttcatgat tctgaaagac gtgaatgaac acatggaagg tctgtttcaa cgtttttcta
       61 aactgttacc tttcagaatt gattttgcct acaggaaaga cacgcccagt tttggtcatt
      121 cctgtaggca cagtatgtgt attgaaatgc accgcttact tagtgaaacc caaacgatgc
      181 tggcgggata ttactgggta atggaatata cgtcaaacaa gggacttcat attcatttta
      241 tcggctacct ggatgggcaa cgtcacaaca aatcttatcg gatatccagg caactgggag
      301 atatctggag gcggatcacg gaaggggagg gttattttca cctgtgcagg gctaaagaca
      361 aatacccggt gcgtatcgat cacgtaattc actatagtga taaatctgcg gtagacgact
      421 tacgttacgc ccttagctat ctagccaaac aggatcagaa agaacacggt attattctga
      481 ggcgtagcag gcttcccgaa aaaagtaacc ggggccgacc acgacataac tgaattacag
      541 ctgtattagg tattagaatt aagtttctgt aagttgttgg gaggtgcttg agttaatagg
      601 cagaagaggg cggggcgctt tgtcatcagt gtaggggacc cgaatttctg ccgtggtgcc
      661 tcagtggatc ggatacaggt aacgaatatg gttcataaat ctgacagtga tgaattagct
      721 gcattgaggg cagaaaatgc ccgtctggtc tcattacttg aagctcatgg gattgaatgg
      781 cgacgtaaac cgcagactcc tgtgcagcgc gtttctgtat tatccaccga cgagaaggtt
      841 gcattatttc gtcggttgtt tcgtgggcgt gatgatgtat gggcacttag atgggaaagt
      901 aaaaccagcg gcaaatcagg gtactctcct gcctgcgcta acgaatggca ggcgagaata
      961 tgcggtaaac cccggataaa atgtggggac tgcgctcacc gtcagttgat tcctgtatcc
     1021 gatctcgtca tctaccacca tctggccggt acccatactg tcgggatgta tccgttgctg
     1081 gaagatgatt cctgttattt tctggcagtt gattttgatg aagctgaatg gcagaaggat
     1141 gcatccgcat tcatgcgatc ctgtgatgag ctgggtgtac ctgctgcgct ggaaatatcc
     1201 cgttcacgtc agggggcgca tgtctggata ttttttgcct cacgagtttc ggcccgcgaa
     1261 gctcgccgtc tggggactgc tattattagc tatacgtgta gtcggacccg acagctgcga
     1321 ttggggtctt atgaccgatt atttcctaat caggatacta tgccaaaagg gggatttggt
     1381 aatctcatag cgttacctct gcaaaaaaga ccgcgtgaat tggggggaag cgtttttgtt
     1441 gatatgaatc tccagcctta tcctgatcag tgggcttttc ttgtatcggt gaccccgatg
     1501 aatgtgcagg atattgaacc gacgatatta cgggctacag ggagtatcca tcctctggat
     1561 gtgaatttta tcaacgaaga agacctgggt acgccgtggg aagagaaaaa atcatcagga
     1621 aacagactga atatttctat tgcagaaccg ctgaaaatca cgctggcaaa ccagatctat
     1681 ttcgaaaaag cgcaattacc tcaggtgctg attaaccgac ttattcggct ggcagcattt
     1741 ccgaaccctg agttttataa ggctcaggca atgcgtatgt cagtctggaa taagccccgt
     1801 gttataggtt gtgcggagaa ttacccgcaa cacattgcgt tgccccgggg atgtctggac
     1861 agcgtattat ctttccttag ggacaacaat attgctgcag aattaatcga taaacgattt
     1921 gccgggacgg aatgtaatgc cgtttttatg ggaaacctca gagcggagca ggaagaggcc
     1981 gtttcggcat tactccgtta tgacactggt gtgctttgtg cgccaacggc ttttggtaag
     2041 acagttaccg cagcggcagt gattgccaag cggaaagtga atacactgat actggtacac
     2101 cggactgaat tgctgaagca gtggcaggag cgtctcgcgg tgtttctgca ggccggtgac
     2161 agtattggta ttatcggggg aggtaaacat aaaccctgtg gcaatattga tattgcggtg
     2221 gtgcagtcca tatccagaca aggagaagtt gaacctctgg tcaggaatta tgggcaaatc
     2281 attgtggatg agtgccatca tattggcgcg gtttcatttt ctgcgattct gaaggaaacg
     2341 aatgccagat atctgcttgg cctgacggca acaccaatcc gacgggatgg tctgcatccc
     2401 attattttta tgtactgtgg tgccattcgc catacagcgg tccgcccgaa ggaaagccca
     2461 cataatctgg aggtactgat ccgttcccgt tttacatctg gtcatttacc atcggatgcg
     2521 agaatccagg atattttcag agaaattgct ctggatcatg acagaacggt ggcgatagct
     2581 gaagaagcca tgaaagcttt cgggcagggg cgaaaagttc tggtactgac tgaacgtaca
     2641 gatcatctgg atgagatagc atcagtgatg aattcactga aattgtctcc ctttattctc
     2701 catggtcgac tatcgaaaaa aaagcgtgcg atgctgatat ccgggctgaa tgctcttcct
     2761 cccgattctc ctcgaatttt gttgtcaaca ggcagactta ttggtgaggg atttgaccac
     2821 cctccgctgg atacgctgat tcttgccatg cctgtgtcat ggaaagggac attacagcag
     2881 tatgcagggc gtcttcacag agagcatacc ggtaaaagcg atgtcaggat cattgatttt
     2941 gtggataccg cgtatcctgt gctgctcaga atgtgggata aacgtcagcg gggttataaa
     3001 gcgatggggt acaggattat agctgacggc gatgaatcag tgatttaatt ttaaggagta
     3061 ctttaccctt tttaaataat ttgagctccc cgggttgttc gggcaacaaa gagtcccagt
     3121 atacaggctt caggaatatg ttgtagtatc aggcttttca tcgctttaaa atgactaccg
     3181 gcagtgagaa cgtcatcaaa aataacaatc tagggcctgc acccttccag taattgttca
     3241 tccagttggt agtttgccat aatcgcttct ggtaagggcc ggtttgttgc ggtatgagag
     3301 gctattgttg atacgttttg cacaaccagt tcccagaaat ccagccatgg ttcgtaggtt
     3361 tgcgcaattc gcagaatttc tgtcatacgg tcatcgtaag cggcatctgt acggcatttg
     3421 gatggtggta cgggaacaaa tgtgtattcc tcaagattac cgatatttga tatgagcatc
     3481 tgagcaacat actcaatagc agccttttta tagtggtaat caggctacga aagtacagcg
     3541 ttttataaat tgtctcaggt gatattcgca gcgttttttg acgtggtttt gttcgcctta
     3601 accatcctga tatttgctct ggagaccatt tcatctccag cttttccaga acaagctttc
     3661 gcaatggtaa attttgatcc agtaagcacg gttttggcct tttcgccatt ctgttggctc
     3721 ggttattagc atcaacagct ttgtaatagc gtctg
//
LOCUS       DBFIFM010000294         3735 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.295, whole genome shotgun sequence.
ACCESSION   DBFIFM010000294 DBFIFM010000000
VERSION     DBFIFM010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.295"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..1058)
                     /locus_tag="N7C74_004542"
     CDS             complement(<1..1058)
                     /locus_tag="N7C74_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGU2434255.1"
                     /translation="MTKVLLSHPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFALERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLLRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRY"
     gene            complement(1068..2249)
                     /locus_tag="N7C74_004543"
     CDS             complement(1068..2249)
                     /locus_tag="N7C74_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGU2434256.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(2317..3342)
                     /locus_tag="N7C74_004544"
     CDS             complement(2317..3342)
                     /locus_tag="N7C74_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU2434257.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1055 a          952 c          907 g          821 t
ORIGIN      
        1 ctatagcgcg acatgctgaa acagaagatc cagtagatca gcgcggcgaa aacataccct
       61 tccgtcgaca tacccaacca ggcgggatca acggttgctt gctgaacgct actgaaaaga
      121 tcgaacaacc cgataatgat caccaggctg gtatctttga agagtgcgat gatggtattt
      181 accagcccag gaattaccag cttcaacgcc tgtggcagaa taaccagccc ctgagttttc
      241 cagtaaccca acgccagcga ctctgccgct tcatattgcc ctttaggcag cgcctgtaat
      301 ccacctcgca cgacttccgc aacatatgct gactgaaaca ggatcacgcc aaccagcgcg
      361 cggagcagtt tgtcgatact ggtgccttct gccataaaca aaggcagcat gaccgaagac
      421 ataaacagaa cggtaatcag cggcacgccg cgccagaatt cgataaaaat gaccgataag
      481 atgcgcacaa tcggcatatg ggagcggcga cctaacgcca gtaagatccc ccacggtagc
      541 gcccccgcaa tcccaactga tgcaataatt aaagttagcg tcagcccgcc ccattgccgg
      601 gtttcaaccc gctcaagagc aaaaaaaccg ccatacatca gccaccagac aatcagtggg
      661 taaatcactg cccagaccgc aatatagcga ccgcgatgcg ggagtatttt ccagaacatt
      721 ggtgcgattg acacaaggcc aatcagtaat gccaagttaa ttcgccagcg ttggtcgtgt
      781 gggtaaagtc catacataaa ctgaccaaat cgttcgtgga tgaagaccca acaggcaccg
      841 gcttttgtgc agtcggcacg cgttgagcca acccagttag cctgcaaaaa tgcccagttc
      901 agcaacggtg ggatcagttc ccacatcaac catatgcagc caatagtcag caggctattg
      961 ctccagctgg agaacagatt ttttcgcacc cacaccatcg cgcggcttga gttatggctc
     1021 gccgggcgcg agggatgaga cagcaatact tttgtcatca tgactcctta gcgttccacg
     1081 atcgcgatgc ggcggttata gatattcatc agcagcgaga tagtcaggct gataatcaga
     1141 tagaccgaca tggtcatggc tatcgtctcg atggcttgcc ccgtctggtt cagcacggtg
     1201 ccagcaaaca gcgaaaccat atcgggatag ccaatagcgg cggcaagaga ggagtttttg
     1261 acgatgttga gatactggct ggttaacggt ggaataatca cccgcagtgc ctggggaata
     1321 atgacctggc gtagcgtaac cgggttgggt aatcccagtg accgagccgc ttcatgttga
     1381 ccataaggca ctgcctggat ccccgcgcgg ataatctcgg cgataaatgc agaggtataa
     1441 accgaaagtg ccagcgttaa ggctgccagt tcaggaatta aaaccatccc gccgcggaag
     1501 ttaaagcctt gtagggctgg gacatcccag tgtagtgctg cgccaaaaag ccattgcgcc
     1561 agtaaaggca aaccgatgat caacactgcg gcgatgggcc aggtgcggcg cagctgtccg
     1621 gtctttatct ggtatgtttt attaaagcgg aatagcccaa cagaaaggac tatagccata
     1681 acaacagcca ggataaacgc aataaatccg tctcccagct gcggtgacgg aatataaagc
     1741 ccacgattgc tcaaaaaggc cagatcaaac gcgctcacag cctggcgtgg tccgggcaaa
     1801 ttgcgcaaca cggcaaagta ccagaagaag atttgcagca gcgggggaat attacggaag
     1861 atctcaatat aaattgtgga aagctttcgt agcagccagt tatccgaaag tctcgccaga
     1921 ccgataaaga agcccaaaac agaagcgaac acaatacaca atgcagaaac cagtagcgta
     1981 ttgagtaagc cgacaataaa aacgcgtccg taggtgtcgc cctgctggta atcgatcaaa
     2041 tgctggacaa taccgaagcc agcgccgcga tccagaaagg caaaacctga agtaatgcca
     2101 cgattattga gattcgttac agtgttgtga aataaccaac caacaatgcc gacaacagca
     2161 acaacggcaa ggatctggaa taaccaggcg cgaaccgtag ggttggcaaa agagagtgag
     2221 cctttaacgg ttgagcggcg atgagacata gaaacctcgg aaagcataac gctgaagaga
     2281 gcgccgccgt agcgacgctc gttacctcac accgacttaa cgcacgggcg gtgcgtactg
     2341 aataccgccg ttattccaga gattattttg cccacgttta attttcagcg ggctttctga
     2401 acctacgtta cgctcgaaaa tttccgagta gttacccacc tgtttgatga tgttataggc
     2461 ccatttatta tccaacttca gatccttgcc gtaatcgcct tctttaccca gcagatgtgc
     2521 catatcaggc gttgctggat tagccgcttt ttcatcgacg ttctgggaat tgatgcccat
     2581 ctcttcagca tttagcatgg cgaaaagcgt ccagcgtaca atcgagaacc attcatcatc
     2641 gccacgacga actaccggcc caagcggttc tttagagata acctccggta agacaatcca
     2701 ttcagccggg ttgcttaatt tgatgcgcag ggcatacagt tgggattgat ccgaagccag
     2761 cgtatcgcag cgaccggatt ccagtgcctt cgctgattcg tcagaacgat cgaaagtcac
     2821 tggtgtgtac ttcatattgt ttgccttgaa atagtcggcg acgttgagtt cggtatcagt
     2881 acccgcctga atacagacgg tagcgccatc cagttctttc gcgcttttta gccccgcttt
     2941 atcgtgcgtc aggaagccaa tgccgtcgta ataagtgacg ccagtaaatg ccattcccat
     3001 cccggcatcg cgagatgaag tccaggtcgt attacgggag agcaaatcca cttcccctga
     3061 ctgtaaagcc gtgaagcgtt cttttgctgt gagtggggta tatttcactt tcgtgtcgtc
     3121 accaaataca gcagcggcaa caccacgaca aacatcaaca tcaatacctg aaaacttacc
     3181 gtctgcatcg gcataagaga acccaggtaa tccatcactg atcccgcatt gcacaaaacc
     3241 ttttttctga acggcatcaa gcgtcgcgcc agcatgcgcc tgatttgcaa cggcaagcag
     3301 cacgctggcg gcagccagtg tggctatcat catctttttc ataatgcttc ctgtgaggcg
     3361 aaaattatcg ttataggacg tttgggaaac gtcttttgcc tgtctgtggc catcctctta
     3421 taagcaaaag tagtgccagc ttttgcgcac gatttcgcgt cgcaataagc tgagtgtaga
     3481 caggaaataa taaaaacagc gccccgcaat ggtgcgaaaa tgaaacatgc gccatatttt
     3541 ggcgcaaaac atttacgcgt gttttactaa gcagaataat gattgttagc gtgagtatat
     3601 ttctggtggg aagtgcgaaa gttaaattga tgcgttattt cgaaagcagc gaaataacac
     3661 gatataacat gctaaatcag cagagaatta gccagcagaa tatagcaata ttttatctta
     3721 tgtaaagata ctgta
//
LOCUS       DBFIFM010000295         3687 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.296, whole genome shotgun sequence.
ACCESSION   DBFIFM010000295 DBFIFM010000000
VERSION     DBFIFM010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.296"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(45..920)
                     /gene="gspC"
                     /locus_tag="N7C74_004545"
     CDS             complement(45..920)
                     /gene="gspC"
                     /locus_tag="N7C74_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HGU2434258.1"
                     /translation="MLFFLSFRGDRGLFIKDIVLKMLTPNRLLCVILLIAGYQLVSVI
                     HHFWLTQAASVPGLSRVSAPETAVTGDQTEERFVFTLFGRASPLSSEGRAQETMPSLS
                     DDLLSGEDLDVRGILYSSVAEHSVAIFAHNNRQFSLSVGEKVPSYDATISAIFSDHIV
                     INYQGKTVSLPLRYDNTEKKNAYDNNNLTVGDVITQDNFRVESVFDIMSFSAVTVNNT
                     LSGYRLIPGKHSSLFYNAGLHDNDLAVSVNGSELRDTRQAQQIMKQLPELKEIKITVE
                     RDGQLYDAFIAVGEN"
     gene            complement(1007..>3687)
                     /gene="stcE"
                     /locus_tag="N7C74_004546"
     CDS             complement(1007..>3687)
                     /gene="stcE"
                     /locus_tag="N7C74_004546"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M66 family glycomucinase StcE"
                     /protein_id="HGU2434259.1"
                     /translation="ERWRTPMKLKYLSCTILAPLAIGVFSATAADNNSAIYFNTSQPI
                     NDLQGSLAAEVKFAQSQILPAHPKEGDSQPHLTSLRKSLLLVRPVKADDKTPVQVEAR
                     DDNNKILGTLTLYPPSSLPDTIYHLDGVPEGGIDFTPHNGTKKIINTVAEVNKLSDAS
                     GSSIHSHLTNNALVEIHTANGRWVRDIYLPQGPDLEGKMVRFVSSAGYSSTVFYGDRK
                     VTLSVGNTLLFKYVNGQWFRSGELENNRITYAQHIWSAELPAHWIVPGLNLVIKQGNL
                     SGRLNDIKIGAPGELLLHTIDIGMLTTPRDRFDFAKDKEAHREYFQTIPVSRMIVNNY
                     APLHLKEVMLPTGELLTDMDPGNGGWHSGTMRQRIGKELVSHGIDNANYGLNSTAGLG
                     ENSHPYVVAQLAAHNSRGNYANGIQVHGGSGGGGIVTLDSTLGNEFSHEVGHNYGLGH
                     YVDGFKGSVHRSAENNNSTWGWDGDKKRFIPNFYPSQTNEKSCLNNQCQEPFDGHKFG
                     FDAMAGGSPFSAANRFTMYTPNSSAIIQRFFENKAVFDSRSSTGFSKWNADTQEMEPY
                     EHTIDRAEQITASVNELSESKMAELMAEYAVVKVHMWNGNWTRNIYIPTASADNRGSI
                     LTINHEAGYNSYLFINGDEKVVSQGYKKSFVSDGQFWKERDVVDTREARKPEQFGVPV
                     TTLVGYYDPEGTLSSYIYPAMYGAYGFTYSDDSQNLSDNDCQLQVDTKEGQLRFRLAN
                     HRANNTVMNKFHINVPTESQPTQATLVCNNKILDTKSLTPAPEGLTYTVNGQALPAKE
                     NEGCIVSVNSGKRYCLPVGQRSGYSLPDWIVGQEVYVDSGAKAKVLLSDWDNLSYNRI
                     GEFVGNVNPADMKKVKAWNGQYLDFSKPRSMRVVYK"
BASE COUNT         1011 a          907 c          729 g         1040 t
ORIGIN      
        1 aacacaagta ccggcaaacg cccattcaac catttcctga acaatcagtt ttctcctaca
       61 gcaataaatg catcatataa ctgaccatca cgctcgacgg ttatttttat ttctttaagt
      121 tctggcaatt gcttcattat ctgctgcgcc tgtctggtat cacgcaattc tgaaccatta
      181 accgatacgg ccagatcgtt atcatgcaac ccagcattat aaaataacga actgtgctta
      241 cccggaatca agcgataacc gcttaatgta ttattaaccg taacggctga aaagctcata
      301 atatcaaaaa cactttctac ccgaaaatta tcttgggtta tcacgtctcc aactgttaaa
      361 ttattattgt catatgcatt ctttttttcg gtattatcat atcgcagagg cagtgataca
      421 gtttttccct gatagttaat aacaatatga tcgctaaaaa tagcactaat cgtagcatcg
      481 tagctgggta ctttttcgcc gacgctcaga ctgaactgtc tgttattatg tgcaaatatg
      541 gcaacggaat gctctgcaac cgaactataa agtatacctc tcacgtcaag atcctcccct
      601 gaaagcagat catctgacag ggaaggcatt gtttcctgcg ctctcccctc cgatgatagt
      661 ggggatgccc tgccgaataa cgtaaaaaca aaacgttctt cagtttgatc accagttacc
      721 gctgtttccg gcgcgctaac acgggataag ccgggcaccg atgctgcctg agtcagccag
      781 aaatgatgga taaccgacac cagctgatat ccggcaataa gtaaaattac gcacaatagc
      841 cggtttggcg tcagcatttt aagtacgata tcttttataa ataagccccg gtcaccccgg
      901 aatgatagaa agaacaacat aataaaacgc tggacgctac tttatgtttt gattaacggg
      961 acgaatgtgt tactaatgcg gccgaagtgg ccgcaccgtc tcaggattat ttatatacaa
     1021 ccctcattga cctaggttta ctgaagtcca aatactgtcc gttccaggct ttaacttttt
     1081 tcatatcagc tgggttcaca ttacctacaa actcaccaat cctgttatag gacaggttat
     1141 cccagtcaga aagcagcact ttcgctttag ccccgctgtc gacatagact tcctgcccaa
     1201 caatccagtc aggaaggcta tatcctgacc gttgaccaac cggcaaacag taacgtttac
     1261 ctgaattcac ggacacgatg catccctcgt tttcttttgc tggaagtgcc tgcccattta
     1321 cagtataggt aagtccttct ggcgcaggtg tgagcgattt ggtatccagt atcttgttat
     1381 tgcaaaccaa tgtggcctgt gtgggctgac tttctgttgg cacgttaata tggaacttat
     1441 tcattacagt gttgttagcc cggtgattag ccagtctgaa tcgcaactgc ccttctttcg
     1501 tatccacctg cagctggcag tcgttatcgg atagattctg actatcatcg gaataagtga
     1561 agccataggc accatacatc gcaggataga tgtagcttga cagcgtgcct tccggatcgt
     1621 aataccccac cagggtcgtc acaggaacac caaactgctc tggcttacgc gcttcacgag
     1681 tatcaaccac atcacgttct ttccagaact gaccatcgga aacaaagctc tttttatacc
     1741 cctgggaaac gaccttttcg tcaccattta taaacagata actattataa ccggcctcat
     1801 ggttgatggt caggatactg cctctattat ctgcggaggc tgtagggata tagatgtttc
     1861 ttgtccagtt accgttccac atatgcactt tgacgacagc gtactctgcc atcagctcag
     1921 ccattttgct ttcacttagc tcattgactg aagccgtaat ctgctccgca cggtcaatgg
     1981 tgtgttcata cggttccatt tcctgcgtat ctgcattcca cttgctgaag ccggtggagg
     2041 aacggctatc gaacacagct ttattttcaa aaaaacgctg gatgatagcc gatgaattcg
     2101 gagtatacat tgtgaaacgg tttgcagcag agaaagggct gcctcccgcc atggcgtcaa
     2161 aaccaaattt gtgtccatca aacggttctt gacactgatt attcagacaa ctcttttcat
     2221 ttgtttgaga cggataaaag ttaggaataa accgtttttt atcaccatcc catccccaag
     2281 ttgagttgtt attttctgca ctacgatgta cagaaccctt gaaaccatct acataatgac
     2341 caagaccata attatgacca acttcatgac tgaactcatt ccccaatgtg gaatctaaag
     2401 taacaattcc cccacctccg gagccaccat gaacctggat gccattagca taattaccgc
     2461 ggctattatg tgccgctaat tgcgcaacta catatggatg actattctcc cctaagcctg
     2521 cggtactatt taaaccatag ttagcattat caatgccatg cgaaaccaat tctttaccta
     2581 ttctttgacg cattgtacca ctatgccacc caccatttcc tggatccata tctgtcaata
     2641 actctccggt tggtaacata acttccttta ggtgtagagg cgcataatta ttaacaatca
     2701 tacgacttac aggaatggtc tggaaatatt ccctatgtgc ttctttgtct ttggcaaaat
     2761 caaagcgatc ccggggagtg gtcaacatcc cgatatcaat tgtatgcaac aacagctcac
     2821 ccggtgctcc aatcttgata tcatttaggc gaccgctcag attgccctgt ttaatcacca
     2881 agtttaaacc aggcacgatc cagtgcgcag gcagttcagc actccaaata tgctgagcat
     2941 aagtgattcg attattctcc agttcaccgg agcggaacca ctgaccattt acatatttga
     3001 acagaagagt gttacccacc gagagtgtga cttttcgatc accataaaaa accgttgaac
     3061 tatagcctgc agacgaaaca aagcgaacca tcttaccttc aaggtcgggt ccctgcggca
     3121 gataaatgtc tcttacccaa cgaccatttg cagtatggat ctccaccagt gcattatttg
     3181 ttagatggct atgaatagaa ctcccgctgg catcactgag tttgtttact tcagccaccg
     3241 tattaatgat ctttttcgtt ccattatgag gtgtgaaatc gataccacct tccggaacac
     3301 catccagatg gtagattgta tccggtagtg atgaaggagg ataaagggtt aacgtaccga
     3361 gaattttatt attatcatcg cgggcttcca cctgaacagg tgttttatca tcagctttca
     3421 ccggacggac aagcagcaga cttttccgca ggctggtcag atgtggttga ctatcccctt
     3481 ctttaggatg ggcgggtaaa atctggcttt gtgcaaattt cacctctgcg gccaacgaac
     3541 cctgcagatc atttataggc tgggaggtat tgaaataaat ggctgaatta ttatcagcag
     3601 ctgttgcaga aaatacccca atcgccagag gggcaaggat cgtacatgac agatacttta
     3661 atttcatcgg tgttctccat ctctcat
//
LOCUS       DBFIFM010000296         3667 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.297, whole genome shotgun sequence.
ACCESSION   DBFIFM010000296 DBFIFM010000000
VERSION     DBFIFM010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.297"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..312
                     /locus_tag="N7C74_004547"
     CDS             <1..312
                     /locus_tag="N7C74_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2434260.1"
                     /translation="PFNIKRCLGKVPVTFVPRTITIIDFVALINGSYCSVPEANVSLS
                     RKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNLKRINELVRHQHIDYL
                     V"
     gene            complement(373..1401)
                     /gene="chuS"
                     /locus_tag="N7C74_004548"
     CDS             complement(373..1401)
                     /gene="chuS"
                     /locus_tag="N7C74_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HGU2434261.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNIREAELAFARVTHDAWR
                     MHGDIREILAALESVGETKCICRNEYAVHEQVGTFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADATVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVAA"
     gene            complement(1450..3432)
                     /gene="chuA"
                     /locus_tag="N7C74_004549"
                     /gene_synonym="shuA"
     CDS             complement(1450..3432)
                     /gene="chuA"
                     /locus_tag="N7C74_004549"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HGU2434262.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASESSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
BASE COUNT          931 a          942 c          857 g          937 t
ORIGIN      
        1 ccctttaata ttaaacgctg cctggggaaa gtgccggtga cctttgttcc gcggactatc
       61 actatcattg attttgtcgc actcatcaat ggcagttact gctctgtgcc tgaagcgaat
      121 gtgtcacttt cgcgcaagca acatcaggtt ctgagctgca ttgcgaatca aatgacaacg
      181 gaagatattc tggagaaact gaaaatatcg ctaaaaacgt tctactgcca taaacacaat
      241 atcatgatga tcctcaatct taagcggatc aatgagctgg tacgccatca gcatattgat
      301 tatctggtgt gaacgattga tcaatataaa gaggcccagc aataaatagc cagacctccc
      361 gttaattata cgttatgcag caacgccttc cggtatcagc gaagcaattt gcttacgcca
      421 ttgcgcttgc tcctgttcgc cttctgtacg ttgaccataa agttgcgcta tctgcgtacc
      481 atcatgggca aacaattcca gactggttac gtagccatcg ctggtcggtt tacgggtaac
      541 ccaggcttca gcaatgctct cttctaatag atgaagagta aacgtcgggt tgaaaatatt
      601 cagccaacct ttcattggca ccactttttc taccacaccg gtgaaaatct gtacgcagcc
      661 acggttgcca acaaacacca tgatttcatt accatcctgc tgtgcagatt caagaatttg
      721 cgccaacgca ctgttggata ctttgcaggc caaatcgtct gccaccagat tgaacgcctg
      781 ttggcgcgtc aggttgtggc gcttgagcaa cgtaaaaaac tgatgaacgt cggtcatcgc
      841 ccgccactct tgctcgacca cagtggcatc ggctcgcgtt tgaacaactg gcgcatcaac
      901 ggcttttaac tcaagcggcg tattctcatc ggtgataaac cgtgccagaa gctcactcca
      961 tgccgccata tcggtattgt cggtggcata aacttttagt aatgcatcgc cctgatgatc
     1021 aaagaactga atactctggc gttcgccacg agccgtgttt tctttgatgt ggaaaacact
     1081 ggcccattga ttgagaaaca gacgtaaatc cagcgcgcgc ggattgagga tcaatccggc
     1141 atgtccgttc aaatgctggt ttgtgaacgt accaacttgc tcatggactg catattcatt
     1201 acgacaaata catttggttt cgccaacact ttcgagcgcc gccagaattt cacggatatc
     1261 gccgtgcatc cgccacgcat cgtgcgtgac gcgtgcaaat gccagttctg cttctctaat
     1321 attcattaac cctgcgatgt cacgcgcgta ctttccggga ttttgttctt ttaactcaag
     1381 ccagcgtgtg tagtggttca ttgtctctct tccttccaga taaatgcccc gaaatatcgg
     1441 ggcagatgat taccattgat aactcacgaa aatttttccg ttacgaccat cctgtgggat
     1501 gccttgcggc gaccagtact ctttgtcgaa agcgttaccc aacaccaaag tagtggtcat
     1561 acctttgagc gcctgttgtc cttgataact gacgtagaaa tcattcacgc catagcctgg
     1621 ttgtttgctg taactgctgc tgatatgtgt tgagcgatcg gcaaacgtac caacccaccc
     1681 aacagagaag ccactgtgag cgatcggaat attcagagtg ctggtaacag tatccgggtt
     1741 aatgctggag atgtattcgc cggtatcggt gtctttgccg cgggtacggt tataggccac
     1801 atcaaggcta aacagatcag tggtatattt cgtcatcaca tcccagcccc agattttggc
     1861 gttcgggacg ttatacgaca tagtcgtcgc cgccgcgaaa tcgacggtcg tggagatgta
     1921 atccttcgct ttggtatcaa agtagctggc tttaaattcc agagcatcat tggacaacat
     1981 caggtcatca aaacgcagcc caaaaccgta ctcctgagtt tcgttagttt ccggacgtaa
     2041 gttcgggttt ggcacccaat agttggtata gaagcgacca atcgagaagt gcttagaatc
     2101 gttatacatt tcgcccatcg tcggggcgcg gaatgcctgg gcatatgagc caaataacat
     2161 cagccagtta gtcggattga tagtcatccc cgcacgagat gaccatttgt cggcatcaac
     2221 atctttgtaa ccgtcactgc taccgcgata actgtcatag cgggttccgc caagcagggt
     2281 aatcggcaga tcgcgtaagg tgatctcatc ctgtagccag ccggagctaa aatcgatttt
     2341 tgcttgcggg aagcccgtcg tcgcgccgcc cggatgttgt tcctgacgat aatactcacc
     2401 gccatatgtc agtaagtgag aagcgaaact gtcggcaaag agagtggaac ggttctccag
     2461 cctggctcct tttgttatct gttcacgata ctcgccggaa ctccccgtgt tttgcgcatt
     2521 aatacggact tccgaccaat aaatttttgc atctgcattt aaccagtcgt tgccctgcgg
     2581 ggcgagttta taagaaagct gcgcatcgcg ttgaattgtt gaacgatcaa ccatcgggtt
     2641 gctgctttca gaagcctcaa cggtctgcgg attttttggt tcacgcgcgt cgttgttgta
     2701 gtaacgcact aaaccgctca gagactgggc tgaatcaatt tgccaggtcc ctttcgccag
     2761 catgttatta atggactcgt cattcggcgc ggtttcacca ttgctctggc gtaaatcacc
     2821 ccgatcgcga ctggaccagg ccacaatacc atccagattt tcagttcgcc caaacgcgct
     2881 cgcgcctaat cccaggctat ggtcccccgt gccgccagta ccaaagacac gaaaaccact
     2941 gctttgtcct tcctgcaata aatcttttgc atcgaccgta tcgtaggaga tcactccacc
     3001 cagcgcgcca ctgccataca gtaatgctga aggtccacga acaatctcaa cacgcttgat
     3061 cagcgccgga tcgagaaaag tgccattcag gtgtccggta tccgttccct gacgaacacc
     3121 atcgacaaga accagcacgc cgcgatgatc atagccacgc atatttacat cctgaccgtt
     3181 ggttcgtccg gtaccatcca gagtaattcc aggaacatga cgcagcagat cggtggctga
     3241 agtagccgtt tgattttcag gagcggaagt gtcgatgacg ctgaccatca taggcgcttc
     3301 gaaggaacta cgggcattcc ccgttgccgt aacggtcatg gtttcagtag caaaagcaaa
     3361 cgttggcaag gtggcagaaa cagctaaggc caataaactc aaacgcaacg aggtaaattg
     3421 cggacgtgac atagcgattc tccatgagga aaataaaaaa tgctggctgc ctgggttaat
     3481 accggggtgg ctggcgaaaa ataaatttat ttaaaattca gagcgataat attcgccggg
     3541 tgagtttaca agcgtttata tcccatgaaa ttgcctgata actcggcaat tacgcaaaag
     3601 cacgccctct ttgtcaggca aaacgatatg ccccatcagg agtgaagaga gctgtgcgtt
     3661 atccctt
//
LOCUS       DBFIFM010000297         3657 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.298, whole genome shotgun sequence.
ACCESSION   DBFIFM010000297 DBFIFM010000000
VERSION     DBFIFM010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.298"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..1324
                     /gene="espX6"
                     /locus_tag="N7C74_004550"
     CDS             <1..1324
                     /gene="espX6"
                     /locus_tag="N7C74_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX6"
                     /protein_id="HGU2434263.1"
                     /translation="ASPEIRSSVLNDLYSIANFLPDNNLTPNESWKRFCETCERFYVA
                     QKSITGDKSERLTRKLSISDAGITMTFKIGDVVINTISTAIPEDATGQRCIEGLNLAE
                     MDLTDIDLSKMALRNVNFNGSILRNAKFSGTICEGVDFTDCDLRNAEFENASLENNDF
                     RKVRHLTYVNFKNANLRNSNFNGKVLTGVTFTGSDLSNAYLEHIDFTTVILYETSKIP
                     GIPGTPQIPGTPKVILTGAILNYSDLSGKDLSEYNLTGILCMYTNFSNANLTNCKISN
                     ANFSNAKFYNTNCTGANCSNILFDYAWFDNTIFIKTLFKNTCFYNVRAKNVYLEGAYL
                     NNDNIVNQANNSTEKQSIDSTDKQANDSTVQQSIDSTVQQANDSTDKQANDNIDKQVN
                     DSTDKQAKNSTEQQDSNSFNQARLKKEVNRRFSIPGLTSYQPTYIVEE"
     gene            1590..2432
                     /locus_tag="N7C74_004551"
     CDS             1590..2432
                     /locus_tag="N7C74_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313321.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HGU2434264.1"
                     /translation="MKKRITFIVFLCAIVAASLFFVQSCVRKSQHVAGFQNYQATIDG
                     KEITGVTKNISSLTWSAQSNTLFSTINKPATIVEMTTEGDLIRTIPLDFVKDLETIEY
                     IGDNKFVISDERDYAIYVISLNADSEVSILKKIKIPLQETPTNCGFEGLAYSSQDHTF
                     WFFKEKNPIEVYKVTGLLRSDELHISKDKTLQRQFTLDDVSGAEFNPQKNTLLVLSHE
                     SRALQEVTVRGDVIGEMSLTKGKYGLSHNIKQAEGIAMDDSGNIYIVGEPNLFYRFTS
                     TKSR"
     gene            2500..>3657
                     /locus_tag="N7C74_004552"
     CDS             2500..>3657
                     /locus_tag="N7C74_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU2434265.1"
                     /translation="MNSTSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMLAE
                     GEFSFSELSWIASGNYAGYLAGSLLFSFGAFHLPSRLRPFLLASALATGLLILAMAWL
                     PPFLLVFIIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGVGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSAIILLALALLIPSNKHVIPPAPLAKIAQQPMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGQPVLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASSSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALICGMQIFK"
BASE COUNT         1009 a          842 c          806 g         1000 t
ORIGIN      
        1 cgcctctcct gaaataagaa gtagcgtcct gaacgatctt tatagcattg ctaatttcct
       61 cccggacaat aatctgaccc caaatgagag ctggaaaaga ttttgtgaga catgcgagcg
      121 cttttacgtt gctcagaaga gtatcactgg agataagagt gaacgtctta cgcgaaaact
      181 ctctatctct gatgcaggaa ttacaatgac cttcaagata ggtgatgttg tcatcaatac
      241 tattagcact gctattcctg aagatgcaac gggtcaacgg tgtatcgaag ggttgaattt
      301 agcagagatg gatttaaccg acatagactt gtcgaaaatg gcgctaagga atgtcaattt
      361 taatggcagc attcttagaa atgccaagtt ctccggtacg atctgtgaag gcgtggattt
      421 taccgattgt gatctgcgta atgcagaatt cgaaaatgcc tcattagaaa ataatgattt
      481 tcgtaaagtt cgccacttga cttatgtaaa tttcaaaaac gcaaatctac gaaacagtaa
      541 cttcaacgga aaagttctca ctggcgtaac ctttactgga agtgacctta gtaacgcgta
      601 tcttgaacac atagatttca caaccgtgat tctatatgaa acatctaaaa tacctggaat
      661 acctggaaca cctcaaatac cgggaacacc taaagtaatt cttactggcg caatactaaa
      721 ttattccgat ctatcgggaa aagatctttc agaatataat cttactggta ttctctgcat
      781 gtataccaac ttttcaaacg ctaatttaac aaattgtaaa atctctaatg caaacttttc
      841 gaatgcaaaa ttctacaata ctaattgtac tggtgcaaat tgttcgaata tcctatttga
      901 ctacgcatgg tttgacaata caatatttat aaaaacgctt tttaaaaata cctgttttta
      961 caatgtcaga gcgaaaaatg tctatcttga gggagcatat ctgaacaatg ataatatcgt
     1021 gaatcaagcc aataacagta ccgagaaaca atccattgac agtaccgata aacaggccaa
     1081 tgacagtacg gtgcaacaat ccattgacag tacggtgcaa caagccaatg acagtaccga
     1141 taaacaagcc aatgacaata tcgataaaca ggtcaatgac agtaccgata aacaagccaa
     1201 gaacagtacc gagcaacagg acagtaacag ttttaatcaa gcccgtttaa agaaagaagt
     1261 gaataggaga ttttccattc cgggtttaac gtcttatcag ccaacatata tagttgaaga
     1321 atagttcatt aatcacccaa acagagtggg tatggcagac gatatagatt aacatttaaa
     1381 cgttaaccgc ctcatctatc attcatagat gaggcgcatt tatcagtcga tgtaagctga
     1441 aaatgttctg ccatccagag acaaccgccg ccccctgcgg cgtttactat ttcatcgccc
     1501 gcgttaagaa ttcattaagc ttcatccgta tactccttat ctgtcgcact gaatttgacg
     1561 agaaaaaagc aataataagg agcaataagt tgaaaaagcg catcaccttt atcgtttttc
     1621 tttgcgccat cgtggcggca agcctgtttt ttgtgcaatc ctgcgtccgt aaaagccagc
     1681 atgtcgcagg gtttcagaat tatcaggcca ctatcgatgg taaagagatt acaggagtaa
     1741 ctaaaaatat ctcctcactg acctggtcag cgcagagcaa taccttattt agcactatca
     1801 acaaacctgc aaccatcgtt gaaatgacga ccgagggcga cttgatccgc accattccgc
     1861 tcgattttgt taaggatctc gagacgattg aatacattgg tgataataaa tttgtcatca
     1921 gtgatgaacg tgactacgcc atttatgtga tttcacttaa cgctgactcg gaagtgagca
     1981 tcctcaaaaa aattaaaatc ccattgcagg aaacaccaac taactgtggc tttgaagggc
     2041 tggcttactc ctctcaggac cataccttct ggttttttaa agagaaaaat ccgatagagg
     2101 tctacaaagt aaccgggcta ttgcgtagcg atgagttgca tatcagcaaa gataaaacgt
     2161 tgcagcgtca gtttacttta gacgatgttt ctggcgcgga atttaaccca cagaaaaata
     2221 cgttgctggt gttgtcacat gagtcacgcg ccctgcagga agtgaccgtg agaggtgacg
     2281 tgatcggcga aatgtcgtta accaaaggta agtatggcct ttcacacaac atcaagcagg
     2341 cggaagggat agcgatggat gatagcggca acatctatat cgttggcgaa ccgaatcttt
     2401 tttatcgctt tacgtcgaca aaatcacgat aacagtctgg caggttctcc ttttttgtta
     2461 ttaacttgtt aactcaaaat aatacaaacc aggcagaaca tgaattcgac atcgcatccc
     2521 gtagaacgtt tttctttcag caccgcgtta ttcgggatgc tggttctgac cttaggtatg
     2581 ggtttaggcc gctttctcta tacgccgatg ctgccagtca tgctggcgga aggcgagttt
     2641 tcatttagcg aactctcatg gatcgccagt ggtaactatg ccgggtatct ggcggggagc
     2701 ctgctgtttt cattcggcgc gtttcattta ccctcacgcc tgcgcccgtt cctgttagct
     2761 tccgccctcg caaccggatt attaatcctc gcgatggcgt ggctgccgcc gtttcttctg
     2821 gttttcatca ttcgctttct ggcgggagtc gccagcgccg ggatgttgat tttcggctca
     2881 acactcatca tgcaacatac tcgccatccc tttgtccttg cggcgctatt ttctggtgtt
     2941 ggcgtcggca tcgctctggg taatgaatat gtgctggcag gcctgcattt tgccctctct
     3001 tcacaaacgt tgtggcaagg tgccggagca ctttctgcca ttatattgct tgctctggcg
     3061 ctgctcatcc cgtcgaataa acacgttatc ccgccagcgc cattggcaaa aatcgcgcaa
     3121 caacccatga gctggtggtt actggcgatt ctgtatggtc tggcgggttt tggttatatc
     3181 atcgtcgcca cctacctgcc gctcatggcg aaagacgcgg gccagcctgt gttgacggct
     3241 cacctctgga cactggttgg cttgtcgatt gtcccaggtt gctttggctg gctgtgggca
     3301 gccaaacggt ggggagcatt accttgcctg accgcgaatt tgctggtgca ggcgatctgc
     3361 gtgctgttaa ccctcgccag cagctctcct ttattactca tcatcagcag tattggtttt
     3421 ggcggcacct ttatgggaac gacctcgctg gtgatgacca tcgcccgcca gcttagcgtg
     3481 ccgggaaatc ttaacctttt gggctttgtg acactcattt atggtatcgg gcaaattctt
     3541 ggcccggcgc tgaccagtat gctcggcaac ggaacgtcgg cgctcgccag cgccacactc
     3601 tgcggcgcag cggcgctatt tatcgcagca ttaatctgcg ggatgcaaat attcaaa
//
LOCUS       DBFIFM010000298         3640 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.299, whole genome shotgun sequence.
ACCESSION   DBFIFM010000298 DBFIFM010000000
VERSION     DBFIFM010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.299"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(106..870)
                     /gene="nfeF"
                     /locus_tag="N7C74_004553"
                     /gene_synonym="yqjH"
     CDS             complement(106..870)
                     /gene="nfeF"
                     /locus_tag="N7C74_004553"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HGU2434266.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1158..1781
                     /gene="nfeR"
                     /locus_tag="N7C74_004554"
                     /gene_synonym="yqjI"
     CDS             1158..1781
                     /gene="nfeR"
                     /locus_tag="N7C74_004554"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HGU2434267.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLNLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(1935..3455)
                     /gene="aer"
                     /locus_tag="N7C74_004555"
     CDS             complement(1935..3455)
                     /gene="aer"
                     /locus_tag="N7C74_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HGU2434268.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
BASE COUNT          852 a         1011 c          916 g          861 t
ORIGIN      
        1 caggcgactc ataatcgctt ggtcgctggt tcaagtccag caggggccac cagatatagc
       61 aaaggctgac gagaaatcgt cagccttttt ctttttatat atcagttact ttgcgtgcca
      121 gtaggccgct gcacgtaccc gctgtgggtc atactgttcc gcttcaaagc ggcggcttaa
      181 attcttaacg actttacctt cgccggttat ccagatgaag taatcatcgg cagggatttg
      241 catctgcgcc agacgcgcat ctaccgcctg ctcatcatgt gccagccatt cgatattaaa
      301 accatcaagg tgcgcgagat aatcctgaca ggcgttatcc cgcacgctaa ccagcgcact
      361 aacttgcggt ttaacggcaa gtttgctcaa cgtttccagg cggcggcgca atgcgggcat
      421 tccggattca tcgcagacat acagctgata cgcgtaatct tccggcacca ccagcgaacc
      481 gcgcggacct gccaccgtaa gtttatcgcc cggttgcgcc tgcatcgccc agccgctggc
      541 gaccccaccg tcgtgaataa agaaatcgat cgccagttca tggcgtagtt cgtcatacag
      601 cggcgtatag tcacgcgacg gtgggcgtgg tccttccggc cagacgatgc cctcttccgt
      661 taccgttggc ggcacaaagt gagcgtcagg ttgaggaaag aagagtttgc tgtgatcgtc
      721 aaagccgcgc gatgtaaaac cgtccagcgc ctcgccgcca aggacaatgc gctgaaaacc
      781 ggcgctgata cgctcaacgc gtagcacagt caattcacgg aagcgcagat cattgcgaac
      841 gcgctgcggg tagcgggggg agttattcat tgttatcgcc ttcgtgatgg taatcagata
      901 tatctaaata attttcgcaa atgataatga ttgttaatca tgataaatgc aagcgatttg
      961 tagaactgat atgtctatag tctgacaaga cgaaccgcct cttctcaggc atcattactc
     1021 aacgccggat gcggcgtgaa cgccttatcc ggcctacgtg tgagatgagt ccacatttaa
     1081 aaatcatttt tattcttgca agagtgctca catcagatat aaattagata tatctaatta
     1141 agcaaaagga ggctgatatg agccatcatc acgaagggtg ttgtaaacat gaaggccagc
     1201 cacgccatga gggctgctgc aaaggtgaga agtcagaaca cgagcactgc ggacacggtc
     1261 accagcatga acacggtcaa tgttgcggtg gtcgccacgg tcgcggcggc ggtcgtcggc
     1321 aacgtttctt tggtcacggt gaattgcgtc tggtgattct ggatattctc tcgcgcgatg
     1381 acagccacgg ttacgaattg attaaagcga ttgagaatct aacccaaggg aattacaccc
     1441 caagcccggg cgtcatctac ccgacgctgg attttctgca ggagcagtcg ctgattacca
     1501 tccgcgaaga ggaaggtggt aagaagcaga ttgcgctgac cgaacaaggc gcgcagtggc
     1561 tggaagaaaa ccgcgaacag gtggagatga ttgaagaacg catcaaagcg cgttgcgttg
     1621 gcgctgcgct gcgccagaac ccgcaaatga agcgggcgct agataatttt aaagcggtgc
     1681 tgaatttacg cgtcaaccag agtgatatca ctgatgcaca aataaaaaag atcattgcgg
     1741 tgatcgaccg cgccgctttt gatattacgc aactggatta atcgccgcat ccgccagtgg
     1801 cgcggtgcaa ttgccggatg cgacgcttga cgcaccttat ccggcctaca cccgctacac
     1861 accccgtagg cctgataaga tgcgccagca tcgcatcagg catcgtgtat caaataccgg
     1921 atccggcata ccgattaatg cagcaccgtc accgcgtctt ccagtcggct ggcgcggtgt
     1981 ttcaccatcg ccgacacctg cgcactctct tccaccagct cggcattttt ctgggtgatg
     2041 aggttaagct catccactgc acgggtcagg ctggaaagac catcggcctg ttccagcgtt
     2101 gaatggctaa tctgggcaat caactgggtg acgtttttca cctgtgccac aatatcttcc
     2161 atcgtacgtc cggcggcgtg tacctgctgc gaaccggatt gcaccttatc agcactggca
     2221 tcaatcagct tgcgaatatc gttggcagca ttggcgctgc ggctggctaa atgacgcact
     2281 tcccccgcca ccaccgcaaa acctttgccc tgttcgccgg cacgcgccgc ttccaccgcc
     2341 gcattcaggg ccagaatatt ggtctgaaac gcaatatcgt taatcagcga agtaatggtg
     2401 ccaatgcgct gggtactgtc ggcgatatcg tccattgtct tgatcaccgt ggtcatcgcc
     2461 tccccgccct gcactgccgc attactggcg gtgattgaca gtttatcggc agccgacgcc
     2521 gtggcagagt tctgtttcac cgacgccgcc atttggttca tggtcgccac cgtttgctga
     2581 acgttatcaa ctgtctgctg ggtatgttcg ttcagttcat cggtgccttt cgccagcgtc
     2641 tcactgccgt ttctgacact ggacacctgg cttgagacat cgttaattaa ccaacggcac
     2701 atcaggccaa gctgccctac cgcgcgtaat gtcagcccca gctcatcgct gcgattcaga
     2761 tgctcaacac tattacgctc tccggtcgcc accttcagtg cctgacgggc gacattttct
     2821 atcgggcgga caatctgcca ttcaaaacag gcgcttgcca acaataccac taacgcacag
     2881 aggatatacg tcaccaccgg ggcagcaaca aaccaaagca tggccgccag caagataaac
     2941 atcagggtca tcactccacg cgcccgccag cgaagcggta atgaaggcag tttacccagc
     3001 cagcctttac gcaccaccag gcctttatga atacgcttac cggtacgtcc ggcgttcagc
     3061 gctttgtaca gcggctccac cgccgcgatc tcttcatccg tcgcccgggt acgaatcgac
     3121 atatagccac tgatttttcc ctcgcgcacc atcggtaccg cattggcccg cacccaataa
     3181 tggtcaccat ttttgcggcg atttttcacg atgccgctcc agggctcccc ttttttcagg
     3241 gtgaaccaca tatccgcaaa cgccgctttt ggcatatccg ggtgacgcac catgttgtgc
     3301 ggctgccctt gtaactcttg caaggtaaag ccgctcacct gcacaaaagt gtcattagca
     3361 tgagtgatat agctttgcag atcggtagtg gacatcagag tggtatcgtc cgccagcggg
     3421 gtattttgct gggtgacata cggatgagaa gacatgttcg cgtcctgtgc aggttatatg
     3481 gttgttaact tcttgtcaga gtttatgtcg gccccgctgc ggttatcttt aaccgattaa
     3541 tttgatttag atcgcaattt gcgattaaag cacaaatcta attcgttgat ttaaaatact
     3601 ttcactctgt tactatacga aaacgttaat tatcttgccc
//
LOCUS       DBFIFM010000299         3636 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.300, whole genome shotgun sequence.
ACCESSION   DBFIFM010000299 DBFIFM010000000
VERSION     DBFIFM010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.300"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..985)
                     /locus_tag="N7C74_004556"
     CDS             complement(<1..985)
                     /locus_tag="N7C74_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HGU2434269.1"
                     /translation="MATWQQGINSGGFLAGIGGQNSNAPKASDVSEALAYIRQNNEME
                     RSGRNNIGLQALQGLGSVAQTSQAAKQQEADAAFQKEYAAAIQSGDRQQVRDLMTKYP
                     GQLEKIQSGMKWADEDQRNSIGTLAAGARLAASSPEAMQSWLQNNAKELARVGVDPNN
                     VAQMYQQNPSGFGEFVYHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGDF
                     EFKSKPSYLVLSFAEISSVVFILMSVYMVAKSQNSNGLQYDALFIPSMAFSILVFSFN
                     GGIISKIISNKVMILLGDASFSFYLVHTIVISTLSKFFNVSGLGAISVIKFI"
     gene            complement(995..1675)
                     /locus_tag="N7C74_004557"
     CDS             complement(995..1675)
                     /locus_tag="N7C74_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HGU2434270.1"
                     /translation="MLVLSESFKNKLLPMNGYMKGGSDSGSKAQARATEKGIELQREM
                     WQTNMQNLAPFTPLAQQYVSQLQNLSSLQGQGQALNQYYNSQQYKDLAGQARYQSLAA
                     AEATGGLGSTATGNQLAAIAPTLGQNWLSGQMNNYNNLANIGLGALTGQANAGQNYAN
                     NVSQLYQQQAAASAANANKPSGLQSFATGAIGGAASGAMIGSAVPVIGTGIGALAGGV
                     IGGLGSLF"
     gene            complement(1662..2129)
                     /locus_tag="N7C74_004558"
     CDS             complement(1662..2129)
                     /locus_tag="N7C74_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HGU2434271.1"
                     /translation="MEIKLIDNPVKLAEFLNNPVNTGNIVDSGDKYYIKPDAVYLGIY
                     EGLVLAGVHEVRNFWHSVVECHAVYDPGFRGEYALQGHRLFCKWLLENSPFLNSITMV
                     PDTTKYGRAIIRLLGATRVGHLDDAYMSNGKPVGITLYQLPRSKYEELLNVST"
     gene            complement(2129..2665)
                     /locus_tag="N7C74_004559"
                     /pseudo
     CDS             complement(2129..2665)
                     /locus_tag="N7C74_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
     gene            2729..3376
                     /locus_tag="N7C74_004560"
     CDS             2729..3376
                     /locus_tag="N7C74_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGU2434272.1"
                     /translation="MVNERSINGQAGKIVSKWVTLANNAIPSNVPPQWDLDLKGQQYS
                     EPTKCIGKQIDYMAKPETKLNDFNREASKSLYDWSDANVWGYSNGWARHEIKKETLSI
                     TGFHAVRRILKAVQASRKDNYKAKRQIQRTLKRQDINRSPITPFQAPLISKHDWDLSV
                     IAVTKEAETERRELLSSQIINSNKVQTHPLLRMLRRARVRASRAKRESGGYDHYY"
BASE COUNT         1021 a          864 c          837 g          914 t
ORIGIN      
        1 ctataaattt aattacactt atagcaccaa gaccagaaac attaaagaac ttgctcaatg
       61 tactgattac tatagtatgg accaagtaaa atgaaaaaga agcatcacct aaaagtatca
      121 taactttatt tgatattatt tttgaaataa taccaccatt aaacgaaaac actaatattg
      181 aaaaggccat tgatggtata aaaagcgcat catactgtaa tccattgcta ttttgacttt
      241 ttgccaccat atagacagac attaggataa agactacaga tgatatttca gcaaatgata
      301 gaactaaata tgacggcttt gacttaaact caaaatctcc ggcctgattg ctgcggattg
      361 tctctgccag cctgcctcgg tcaatctcac gaccagccat cttgtcctga acattgaagt
      421 agtcaatcgg accgagagca gccatcccaa ggtgataaac aaactcacca aaccctgaag
      481 gattctgctg atacatctga gcaacgttgt tagggtcaac accgacgcgc gccagttcct
      541 tggcgttgtt ttgcagccat gattgcattg cttctggaga cgaggccgca aggcgtgcgc
      601 cagccgctaa ggtgccgata gaattgcgct ggtcttcgtc tgcccacttc ataccagact
      661 gaatcttctc taattgacca ggatatttgg tcatcagatc tcgaacctgc tgtcgatcgc
      721 cggactggat ggctgccgca tattcttttt ggaatgcagc atccgcttcc tgttgctttg
      781 cggcttgaga tgtttgagcg acactaccaa gtccctgcaa cgcctgaagg ccgatgttat
      841 tgcgacctga acgctccatt tcgttgttct ggcgaatata ggccaacgcc tcacttacat
      901 cacttgcctt tggcgcattt gagttttgcc caccgatacc agcaagaaaa ccgcctgagt
      961 tgattccttg ttgccaagta gccatattcc caccttaaaa caatgatcca agaccaccga
     1021 taacaccgcc agcaagagca ccaataccag tcccaataac aggaactgca ctaccaatca
     1081 ttgcacctga tgcggcccca ccaatggcac ctgtagcaaa actctgtagg cctgaaggct
     1141 tattcgcatt tgctgccgat gctgccgcct gctgttgata caattggctg acattgttag
     1201 cgtagttctg tccggcgttt gcctgacctg taagagcacc aaggccgata tttgccagat
     1261 tgttgtagtt gttcatctga cctgacagcc agttttgacc gagtgtaggt gcgattgctg
     1321 ctaactggtt tcctgttgct gtagagccta atccacccgt tgcctctgct gctgccagac
     1381 tctgatagcg cgcctgccct gcaaggtctt tatactgctg agagttgtaa tactggttaa
     1441 gcgcctgacc ttgcccctga agagaggaaa gattctgcaa ctgtgatacg tactgctgag
     1501 cgagtggcgt gaacggtgca aggttttgca tgttcgtctg ccacatttca cgctgcagtt
     1561 cgatgccctt ttcagttgcg cgtgcctggg ctttagatcc ggagtcgctg ccgcctttca
     1621 tatacccatt catgggaagc aatttattct tgaagctttc gctaagtact aacatttaat
     1681 agctcctcat atttcgaacg aggtaattga tagagggtga ttcctaccgg ttttccgtta
     1741 ctcatgtacg catcatcaag gtgaccaaca cgggtagcgc caagcaaacg gataattgcc
     1801 cgtccgtatt tggtggtgtc aggaaccatg gtgatgctgt taaggaatgg tgagttttcg
     1861 agaagccatt tgcagaataa tcgatgccct tgtagtgcat attcgccacg gaatccgggg
     1921 tcatacaccg catggcattc aacaacgcta tgccagaagt tacgcacttc atgaactcca
     1981 gccagcacta atccttcgta gatgccgaga tataccgcat caggcttgat gtagtattta
     2041 tctccactgt ctacgatatt ccccgtgttt accgggttgt taaggaattc tgcaagtttc
     2101 accgggttat cgatgagctt tatttccatc actgctccgc aatgattttg atggttgtgg
     2161 cagtaaacgc cgcgccattc gactgaatgg ttaacgtgct gccatttgtg gcaagaaagc
     2221 cgtctttatc cacgctgaag aacgtagcta acaggatgtt gtcggttgtt gttgccgagt
     2281 tgcgactgct tactagcgtg tcaggaacag aaccggagaa tgttagctgc attgacctgt
     2341 tggcggttcc gctgggccac gtcccgacaa tcgacagctt gaagaacaag gttttgttct
     2401 cgttgaacac aaccatcttg ttgttaacgg tgtcgaagaa tggtgccaac gtcccggatg
     2461 acggcgtaag cgttttcagc aggctaacaa ggttggtcgg cgctgtcggg atggttactg
     2521 atacgccaga gtaaacaacc tctgacttct tgcgcgtagt ggcatactcc agagcattaa
     2581 tgcgcgtttc atggtctgaa acctgcgatt ccagcgactg aactctggta tcaagcgatg
     2641 caatatcgct ttcattcaag gcaatcagtg ggtttacgtc atggagtggg ctcaacacca
     2701 gtcagagccg catgtacacg caataatcat ggtgaacgaa agaagcatta atgggcaagc
     2761 tggcaaaatt gtcagcaaat gggtaacact ggcaaacaat gcaatcccat caaatgttcc
     2821 tccccagtgg gatctagatt tgaaaggtca gcaatattca gagcctacaa agtgtatagg
     2881 taaacagatt gattacatgg caaagcccga aactaagtta aatgacttta atagagaggc
     2941 gagcaagagc ctatatgatt ggtcagatgc caacgtttgg ggttattcga atggctgggc
     3001 gcggcatgag ataaaaaaag aaacactttc tatcactggc tttcatgctg taagacgcat
     3061 actaaaagcc gttcaggcat caaggaaaga caactacaaa gctaagcgac agatacagcg
     3121 tacattgaaa cgacaagaca taaatcgatc accaatcacc ccgttccaag caccgttaat
     3181 tagcaaacac gattgggatt tatcagtgat agcggtcacc aaagaggcag agacagagcg
     3241 cagggaatta ctatcctcac agataataaa ctcgaacaaa gtacaaaccc acccactgtt
     3301 aaggatgtta agaagagcac gagtgagagc atccagagcc aaaagagaaa gcggaggtta
     3361 tgaccattac tactagtagt gacagatcgt gacaaggcgg tgcaaaaaac gaccaataat
     3421 aagagagata tgcctgccaa accgccaaaa gcgcggagcg cggcaaacgc cgcgagaggc
     3481 gcaagaaggc gaacctatcc attgaaacgg tcagaagcgc atcgcgctcg acgaccgaag
     3541 tccccactaa caccgttaac gaaagatcaa agtcttagaa aaaaatgacg tttctttgat
     3601 ggacttccat ccgtaaaaat caaattttga gaaaaa
//
LOCUS       DBFIFM010000300         3631 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.301, whole genome shotgun sequence.
ACCESSION   DBFIFM010000300 DBFIFM010000000
VERSION     DBFIFM010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.301"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(178..543)
                     /locus_tag="N7C74_004561"
     CDS             complement(178..543)
                     /locus_tag="N7C74_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU2434273.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGHGRADDIIGL"
     gene            585..812
                     /locus_tag="N7C74_004562"
     CDS             585..812
                     /locus_tag="N7C74_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU2434274.1"
                     /translation="MSSKNRPRRTTTRNIRFPNQMIEQINIALDQKGSENFSAWVIES
                     CRRELAADIKYARQLTIKKNDTQYALRWLFI"
     gene            complement(1275..1532)
                     /locus_tag="N7C74_004563"
     CDS             complement(1275..1532)
                     /locus_tag="N7C74_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434275.1"
                     /translation="MSDRVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(1529..2026)
                     /locus_tag="N7C74_004564"
     CDS             complement(1529..2026)
                     /locus_tag="N7C74_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HGU2434276.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGAHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELHHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPGLIAEINSLANQLQISI
                     PGLPG"
     gene            complement(2229..2666)
                     /locus_tag="N7C74_004565"
     CDS             complement(2229..2666)
                     /locus_tag="N7C74_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU2434277.1"
                     /translation="MSRVTVIISALVICIIACLSWAVNHYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVSATCAKSTTGASSM
                     GDGESPGLTADAELNYYRLRGGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(2663..3160)
                     /gene="rrrD"
                     /locus_tag="N7C74_004566"
     CDS             complement(2663..3160)
                     /gene="rrrD"
                     /locus_tag="N7C74_004566"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HGU2434278.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYINVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(3160..3375)
                     /locus_tag="N7C74_004567"
     CDS             complement(3160..3375)
                     /locus_tag="N7C74_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU2434279.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            complement(3452..>3631)
                     /locus_tag="N7C74_004568"
     CDS             complement(3452..>3631)
                     /locus_tag="N7C74_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU2434280.1"
                     /translation="HNLEARLDAEVDAILDELLGVQAEPPTEAGDTTAESGEVQPESP
                     VADATEPQPESVMML"
BASE COUNT          893 a          978 c          832 g          928 t
ORIGIN      
        1 gttaacattt tttccgcaca acaaactgaa taataaagat aaaaaccgca aaaatgcccg
       61 gacagccagt taacatgtta actgccctga aacgggaatt ttttctctgc gtgagacggg
      121 gggcggtgtc cggagcgatc gtttttttcg ccggatgatc cccccccccg gggcgggtca
      181 cagtccgatg atatcgtctg ccctgccatg acctccggac acctccggca gcgtcgggtc
      241 cggcatacca cccgccgctt cacgagcaga cttttgtcga tggcattcgg tacagagcgt
      301 ccagagattc gtctcctcat taccaccacc gaactgaagt gcaattcggt gatcgagttc
      361 actgtcacag aggtcaacca cacgaccaca gagacagcac tgcccggcat ccctgagcca
      421 gatatgacgc ttgagggaaa cacgtgcact gccactgaca cgacgctgtt cacccttcag
      481 aatattcacc cgccgggtgt tcagagtttt gattctgccc ggtaacgtac gaagcacagc
      541 catgtaaaat cctcgccata tagcttgtca ccagaggaaa gaaaatgtca tcgaaaaacc
      601 ggccccgcag aacaacaacc cgcaacatcc gatttccaaa ccagatgatt gaacaaatta
      661 acatcgctct tgaccagaaa ggttcagaaa atttttctgc gtgggtcatt gaatcttgcc
      721 gccgggagct ggcagcagac ataaaatatg cccgtcagtt gactataaaa aagaatgata
      781 cacagtatgc tctgcgatgg ctgttcatat aactatttct ttatattgct gaatttataa
      841 aaactcacag acattagctg tattaattcc gaattgaaat aatcagccat atagaataaa
      901 aataaagcat aacaataata atcttctacc caatcagtac attactgctg tgactccaac
      961 acggcagttt ttttattgaa cagattccag tttcttccac catcgcaccg gacgggcgac
     1021 catgagggga gaacgccgcg ctccgtttac gcggtaaacc ccggtgtgta tcgtttttga
     1081 ttatccccgc acactcgcgc agaaggaatt ccccgtcggg ctacggtcat ggttaatgcg
     1141 gaaatacagc gacgatacag cgcatgatgt gtcaggcttg aataccttta tccgttaaaa
     1201 gggatatcag ttaagttatc ccgtgtaggg tataagccat tatcaagccc accagtagat
     1261 gggctttgta atggctactt cgcttttgct tccgctcgct tacgccggcg ctcttctttc
     1321 ctctcggctt ttgccatgtc catgaatgcc tgcatgatcg agttccgcat catgtagcta
     1381 acaaagtgat gattgacaca gccgttgagg cgcagctgct cgccaaactc atccaccgag
     1441 gccaatgctt ccatcatacc cttctcgcct ttcatgaact ctgagaagtc gcgccccgct
     1501 ctggaggcgc attcaatgac acgatcactc atcccggaag cccggggatc gaaatctgca
     1561 actggttagc cagggagtta atttcagcga tcaaaccagg tttcgtgtag cgccatgccg
     1621 cgaggccttg tccacagaag ctcgccatgt cttttttctg atcaaactca tgacatttca
     1681 tgttgagctg cgcacttaag ctgttccgat gatgaagctc tccggtgaag taatcatcga
     1741 ggactttata ggctgcatat ttaaatccgg ggtttagcca tgctgcgtaa tcataagcaa
     1801 cgaactttcc gccataagtt ccaccgtgcg caccgcgttc cgtaaaaacc acagattcgt
     1861 ggtttttctc cagctcagcc aagaactctt tggtctgctt gtttcgcagg tagtggtacg
     1921 gagattcaga ttcactttta ccactggctt tccacatatc agtgaggcag atcatgccat
     1981 cttcaccgat acggattggt tgattgaaga gggttaatga tttcatagcg tgtacctact
     2041 ctttgaaatg aacctttgcc gcacaggaaa ccagcccacc gaggctcgcc agcactaact
     2101 ggtatcctca aaggcccatt ccaaaggggc aggttcggtg taaaaaacat gcgttgcggt
     2161 acgcatttat tgcaaaaagc cccgcatcgc gaggctcatt aaatggactt tgtgatttgc
     2221 aaaaaaaatt atttcaggca ttgcgtcctg atgtactcct gcaggtagtt aacctgcgcg
     2281 gttatcttgt cgattccacc tcggagacgg taataattga gttcagcatc tgctgtaagt
     2341 cctgggcttt ctccatcgcc catgctgctg gctccggtcg ttgactttgc acaggtggcg
     2401 gagacttgca ggcgcttacg cccagcagaa acatcagcac ggagactttc gatagtcgca
     2461 ttagcatcag caagctcctt tgtgtatctg gcgtcaagtt ctgctacatc acgttgccgc
     2521 ttctgcatat cagcgatgat ggatgtggct ttatcgcgct gctctttgta ggcgatggcg
     2581 ttatcacggt aatgattaac agcccatgac aggcaggcga taatgcagat aaccagagcg
     2641 gagataataa cggttaccct gctcattgtt gcccccacaa acagacttca cgctcaatct
     2701 cgcggcgagt catcagccct ttccattgct taccgccagc gtatgtccag cgccgtagct
     2761 gatcacatgc gcctttgata tcgccctggt ttattttgcg aagaagcgtc gatgttctga
     2821 aattgccagc gcccacgttg taaacgaacg agtaaagagc gccgcgcgtt gtttccggta
     2881 tatcgacgtt gatgtacggg ttaatttgtc tggcgaccgt ggcaaggtct ttattcagga
     2941 gggctttgca ttctgcttcg gtatacgttt taccgggcat gatgtctttt ccggtgtgtc
     3001 cgtgacatac agtccatacg ccaacgatat ctttgtatgg tatgtagctg acaccttcca
     3061 gaccatcgtt accacctgga ccagtgatga gcacagacgc tatggcaaca gccccaccac
     3121 caatagcagc tgcaacagcc ttgcgtaatg acggcgacat tattcacctc tcgcagcctt
     3181 acgcttatct tctttaatct tgaaataaag atttgtcaga tacgtcagca ggccaaacag
     3241 cagacttccc agcacaccta ttgccaccca ctgggacgga gagactttgt ccagcagctg
     3301 cagtaaccag tatcccgtcc ccaccgctga cgtggtgtat gacacacctg ttgtgatttt
     3361 ttccatctgg tacatacccc gtctcccgtt atccggaagc tcacaacatg aaaaaggcca
     3421 gcagctgttt actgatggcc ctgactcccc gttacagcat catgaccgat tcgggttgag
     3481 gttcagtcgc atcggcgacc ggtgattcag gctgaacttc accgctctct gcggtggtat
     3541 ctcccgcttc agtcggtggc tctgcctgta caccaagcag ctcatccaga atggcatcaa
     3601 cctctgcatc aagacgcgct tccaggttat g
//
LOCUS       DBFIFM010000301         3609 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.302, whole genome shotgun sequence.
ACCESSION   DBFIFM010000301 DBFIFM010000000
VERSION     DBFIFM010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.302"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            3..671
                     /gene="fimC"
                     /locus_tag="N7C74_004569"
     CDS             3..671
                     /gene="fimC"
                     /locus_tag="N7C74_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HGU2434281.1"
                     /translation="MLIIFYLIISASVHAAGGIALGATRIIYPADAKQTAVWIRNSHT
                     NERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAADRESLFWMN
                     VKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSRSGNYINVH
                     NPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDYGSVTPVRE
                     VNLN"
     gene            702..3311
                     /locus_tag="N7C74_004570"
     CDS             702..3311
                     /locus_tag="N7C74_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HGU2434282.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTFVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            3324..>3609
                     /gene="fimH"
                     /locus_tag="N7C74_004571"
     CDS             3324..>3609
                     /gene="fimH"
                     /locus_tag="N7C74_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="involved in the regulation of fimbriae length and
                     mediates adhesion of type 1 fimbriae; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HGU2434283.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNY"
BASE COUNT          950 a          791 c          874 g          994 t
ORIGIN      
        1 tgatgctcat tatattttat ttaatcattt cggccagcgt ccatgctgcc ggagggatcg
       61 cattaggtgc cacgcgtatt atttatcccg ctgatgctaa acagactgcg gtatggatta
      121 gaaatagcca taccaatgag cgttttctgg tcaattcgtg gattgaaaac agcagcggtg
      181 taaaagaaaa gtcattcatc attacaccgc cactatttgt tagtgaaccc aaaagcgaaa
      241 atactttgcg tattatttac accggtccac cgctggcagc agatcgtgag tctctgttct
      301 ggatgaatgt taagacgatt ccttcggtag ataaaaatgc attgaacggc aggaatgttt
      361 tgcaactggc gattttatcg cgcatgaagt tatttctccg tccaattcaa ctacaagaat
      421 tacccgcaga agcgccggat acactcaagt tttcgcgatc cggtaactat atcaatgttc
      481 ataatccatc acctttttat gtcaccctgg ttaacttaca agtgggcagc caaaagttgg
      541 ggaatgctat ggctgcaccc agagttaatt cacaaattcc cttaccctca ggagtgcagg
      601 gaaagctgaa atttcagacc gttaatgatt atggttcagt aaccccggtc agagaagtga
      661 acttaaacta accgaatcat ctgacaatat cagagctaat tatgaaaata cccactacta
      721 cggatattcc gcagaggtat acctggtgtc tggccggaat ttgttattca tctcttgcca
      781 ttttaccctc ctttttaagc tatgcggaaa gttatttcaa cccggcattt ttattagaga
      841 atggcacatt cgttgctgat ttatcgcgct ttgagagagg taatcatcaa cctgcgggcg
      901 tgtatcgggt ggatctctgg cgtaatgatg agttcattgg ttcacaggat atcgtatttg
      961 aatcgacaac agaaaataca ggtgataaat caggtgggtt aatgccctgt tttaaccagg
     1021 tactccttga acgaattggc cttaatagca gtgcatttcc cgagttagcc cagcagcaaa
     1081 acaataaatg catcaattta ctgaaagctg tacctgatgc cacaattaac tttgattttg
     1141 cagcgatgcg cctgaacatc actattcctc agatagcgtt gttgagtagc gctcacggtt
     1201 acattccgcc tgaagagtgg gatgaaggta ttcctgcttt actcctgaat tataatttca
     1261 ccggtaacag aggtaatggt aacgatagct atttttttag tgagctcagc gggattaata
     1321 ttggcccgtg gcgtttacgc aacaatggtt cctggaacta ttttcgcgga aatggatatc
     1381 attcagaaca gtggaataat attggcacct gggtacagcg cgccattatt ccgctgaaaa
     1441 gtgaactggt aatgggagac ggcaatacag gaagtgatat tttcgatggt gttggatttc
     1501 gtggtgtacg gctttattct tccgataata tgtatcctga tagccagcaa gggtttgccc
     1561 caacggtacg tgggattgcc cgtacggcgg cccagctaac gattcggcaa aatggtttta
     1621 ttatctatca aagctatgtt tcccccggcg cttttgaaat tacagatttg cacccgacat
     1681 cttcaaatgg cgatctggac gtcaccatcg acgaacgcga tggcaatcag cagaattaca
     1741 ctattccgta ttcaacagtg ccaattttac aacgcgaagg gcgtttcaaa tttgacctga
     1801 cggcgggcga ttttcgtagc ggtaatagtc agcaatcatc gcctttcttt tttcagggca
     1861 cggcactcgg cggtttacca caggaattta ctgcctacgg cgggacgcaa ttatctgcca
     1921 attacaccgc ctttttatta gggctggggc gcaacctcgg gaactggggc gcagtgtcgc
     1981 tggatgtgac ccatgcgcgc agtcagttag ccgacgacag tcgtcatgag ggggattcca
     2041 ttcgcttcct ctatgcgaaa tcgatgaaca ccttcggcac caattttcag ttaatgggtt
     2101 accgctattc gacacaaggt ttttataccc ttgatgatgt tgcgtatcgt cgaatggagg
     2161 ggtacgaata tgattacgat tatgacggtg agcatcggga tgaaccgata atcgtgaatt
     2221 accacaattt acgctttagc cgtaaagacc gtttgcagtt aaatatttca caatcactta
     2281 atgactttgg ctcgctttat atctctggta cccatcaaaa atactggaat acttcggatt
     2341 cagatacctg gtatcaggtg gggtatacca gcagctgggt tggcatcagt tattcactct
     2401 cattttcgtg gaatgaatct gtagggatcc ccgataacga acgtattgtc ggacttaatg
     2461 tttcagtgcc tttcaatgtt ctgaccaaac gtcgctacac ccgggaaaat gcgctcgacc
     2521 gcgcttatgc ctcctttaac gccaaccgta acagcaacgg gcaaaatagc tggctggcag
     2581 gtgtaggtgg gaccttactg gaaggccaca acctgagtta tcacgtaagc cagggtgata
     2641 cctcgaataa tgggtatacg ggcagcgcca cggcaaactg gcaggccgct tacggtacgc
     2701 tgggggtcgg gtataactac gaccgcgatc aacatgacgt taactggcag ctgtctggcg
     2761 gtgtggtcgg acatgaaaat ggcataacgc tgagccagcc tttaggggat accaatgttt
     2821 tgattaaagc gcctggcgca ggcggtgtac gcattgaaaa tcaaactggc attttaaccg
     2881 actggcgcgg ctatgcggtg atgccgtatg ccacggttta tcggtataac cgtatcgcgc
     2941 ttgataccaa tacgatgggg aattccatcg atgttgaaaa aaatattagc agcgttgtgc
     3001 cgacgcaagg cgcgttggtt cgtgccaatt ttgatacccg cataggcgtg cgggcgctca
     3061 ttaccgttac ccagggcgga aaaccggtgc cgtttggatc actggtacgg gaaaacagta
     3121 ccggaataac cagtatggtg ggtgatgacg ggcaagttta tttaagtggt gcgccattgt
     3181 ctggtgaatt actggttcag tggggagacg gcgcgaactc acgctgcatt gcgcactatg
     3241 tattgccgaa gcaaagctta cagcaagccg tcactgttat ttcggcagtt tgcacacatc
     3301 ctggctcata aaggaaatta tcaatgaaga taatctgcag attattattg gcgatggcat
     3361 gtttgtgtct ggcaaacata tcctgggcta ctgtttgtgc aaatagtact ggcgtagcag
     3421 aagatgaaca ctatgatctc tcaaatgtct ttaatagcac caataaccag ccagggcaga
     3481 ttgttgtttt accggaaaaa tccggctggg taggtgtctc agcaatttgt ccacccggca
     3541 cgctggtgaa ttatacatac cgtagttatg tcactaactt tattgttcag gaaactatcg
     3601 ataattata
//
LOCUS       DBFIFM010000302         3491 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.303, whole genome shotgun sequence.
ACCESSION   DBFIFM010000302 DBFIFM010000000
VERSION     DBFIFM010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.303"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..254
                     /locus_tag="N7C74_004572"
     CDS             <1..254
                     /locus_tag="N7C74_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGU2434284.1"
                     /translation="SGDSDFIFYNNLSSPEGAVRLVTGSQQASIEIALDRVPANVSKI
                     AITVVIDGEDTISSGCTLTDKMAIEKVQLTYLDLVVARW"
     gene            400..550
                     /locus_tag="N7C74_004573"
                     /pseudo
     CDS             400..550
                     /locus_tag="N7C74_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(768..2858)
                     /gene="iha"
                     /locus_tag="N7C74_004574"
     CDS             complement(768..2858)
                     /gene="iha"
                     /locus_tag="N7C74_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HGU2434285.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            2797..2979
                     /locus_tag="N7C74_004575"
     CDS             2797..2979
                     /locus_tag="N7C74_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434286.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     ICLLQKIKIIDKTKYK"
BASE COUNT          876 a          895 c          799 g          921 t
ORIGIN      
        1 tcagtggcga ttctgacttt atcttttata ataatttgtc ttctcctgag ggggcagtca
       61 ggctcgttac cggttcgcag caggccagca ttgagatagc actggatcgt gttccggcga
      121 acgtcagtaa aattgcgatc accgttgtca ttgatggtga agataccatc agttcaggct
      181 gtacgcttac tgacaaaatg gcgattgaga aggtgcaatt gacttatttg gatttggtcg
      241 tggcgaggtg gtgaccctgc attctgctaa ccgggttatg agtcaggagt cagcgataga
      301 caaagggact tatctgaccg ggcccctgga aaaatggcgg gagagaatga taccttcctg
      361 ctgacgagac cggactttga gatgacgagc gatagtgcgg cagtgatgta cagcctgatc
      421 ggtagctgca aaacttaacg gaatcgagcc ggaaacgtgg ctacgccacg tgatcagtgt
      481 aatcaacacc tggcctgcca accgcatgaa agagttgttg ccctggaacg tcactctctc
      541 tgtaaactaa tttttacccc acgtccttca cggtgcgctt acactctgga caagtatgta
      601 aatgataagt taaaaataat ccgtgcattt cctgtgacga tattagttgt tggtaacgaa
      661 atctcattag cggatcggaa atgcacttaa cctaatacgt tgccactgtt ccgccaggag
      721 ttgcagtggc aacgtattct accgtcagtg atagcgtttt gttattatca gaactgatag
      781 ttcagcgaca tccagtaatt tcgctcaggt atcacatatc ctgttgttga tgatcccgtc
      841 tggaagtaat caccggcata cagcgtactc ttaccggcac tgtacaggct cacgtcactg
      901 taatccttgt tgagcaggtt attcaccgca gcattcagcg tcagggcatc cgtcatcttc
      961 cacgacagac ctgcatccac caccgtccag gctttcaggt attctccttt ctcatcatac
     1021 actttcttct gtacagcgct cagtgacgaa taattctggg tgaaacgtgg tgttttcccg
     1081 cgataacggg cacccagcca tgatgccacc tcttcggtga tctgccagtt cagtttcgca
     1141 ttcaccatgt gttcaggggt ataactcagc ggcgcacctt tgttatcacc atcacgttgt
     1201 tcacttcggg tccaggtgta attcagtgac agcgtgacat cctctgacca cagcggcaat
     1261 gtgccggcaa attccacacc gtgcaaccgg gcctttccgc tgtttacata gctattggtg
     1321 ttatcattta tggaataaga gacaatcttg ttggagaagt cagtcataaa acctgtgaca
     1381 ttggcattca gaccggcggg gttatcgtaa tacaccccag cctcataact gacgctctct
     1441 tccggcttca ggtcggggtt accaagtaga tttgtttttc cctgcccgga cacaccacta
     1501 atccctttat gtagctgccc cattctgggt gccttatatc ccgtggtcac accgcctttc
     1561 agcgtccagg catctgccac atcccagacc agatatgcac gcggactgaa gtgtcccccg
     1621 aattgctcat gatgttcata gcggctgccc gcagtcagcg caagtgcatc cgtgagatgc
     1681 cactcatcct cagcaaatac cgaccagctt ttctgccgga aagtttcacc tgtgctggca
     1741 aggacaactc cgtctttcat ggacgagctc tgaaactcgc cccccaccgt aaccagatga
     1801 gattctccca gaggggtaag cagtaatgaa ttcaggataa ggttcgattc cttaagctcc
     1861 cgcggctgac cggcaagccc ccatttgtcg cgcttcagta cactgcgtac aagctcacga
     1921 cctttatttt ctgtctcgtt ccagttcaga tacgatttcc atgttccgaa ggtgaaagta
     1981 tgatcatagc cagctgaaat tttgtttcgc tcatagcgca gggtccggtc atatcccccc
     2041 gtcagactcc ccagttgccc atcccggtta tcataacgct gccgggtggt atccatatca
     2101 aaccagagca catcctgctc cgacgccttc cagtcaagac gtgcaccaag attataattc
     2161 tgtgactccg tgggataagg aatacgcgtg gctgctgtat cgctcagtga tgtgaccgat
     2221 gaaccctgac gctgttgtgt gctaccgcgt acctgcaggc tgacagaatc atccacaagg
     2281 ggaccactgc tccagaaatt aaactggctg ctgttacccc atttgttgct ttcctgcaga
     2341 ttcagccctg cattgacgga agagagccat ttgtctgcat tctttctggt aatgatattc
     2401 accacaccgc ccatcgcatc agagccatac agtgtggaca tcggccccct gataacctca
     2461 atacgctcaa tggcggccag agggggcatg aacccggtat tcatggcaga aaaaccgttg
     2521 ggagtcacgt cactgcttcc gccctgacga acaccatcaa tcagtatcag cgtgtaactg
     2581 gctggcattc ctcggatgct gatttccagc cctccggttt tacccgtacc actttcaaca
     2641 tccacaccct ctactgatct cagagcctcc gccagatcgt ggtactggct ggactgcaat
     2701 tcctcctggc taatcacaga aacactggcg gctgcgttag tcagcttttt ctcatagccg
     2761 gatgccgaga caatcatcac atcctctgca gcagctatgc tgctggctga aaatccgaga
     2821 cagggaatga ctacggaagc cagagtggtt attcgcatat tactaatctc cgatcatgtt
     2881 aaccggcaag acaatacaca aatacaaaca ataatgagaa taatttatat ttgcctttta
     2941 caaaaaataa agattattga caaaaccaaa tataaataac gactgcctcg gcatgccagt
     3001 taacagctat ccgtatggcg tgttcggttt tgcgaataaa aaatggttac tgtagaagcg
     3061 gatttccacc acatgtcaac catgaatggt cagtgatgcg agacgactgg gatgatttgg
     3121 tcgatgactt cttccggcga atactgatag aaacattgaa atattgtaag agaaagcgtt
     3181 caaaattctg tggcacgtga aaaattctac agcgtcatca ccttattcag tcgaccttca
     3241 aaataaacgt ttaacagaga caatcaccat ttggattgtc cgctgaggag gcgaacaatt
     3301 tcctagacag ctttatcatg tcagcccaca ttgttggcgc tggcaatact aaggacaaac
     3361 gatggggaag tagcttttta gcgttatttt cgggagtaaa aaacgtggta tctttccgta
     3421 tgtcagttgt atcttgctgg tattcgtagt cgtgataagg catacaacga tatttatctt
     3481 gacgttagtg a
//
LOCUS       DBFIFM010000303         3490 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.304, whole genome shotgun sequence.
ACCESSION   DBFIFM010000303 DBFIFM010000000
VERSION     DBFIFM010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.304"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(133..429)
                     /gene="yciI"
                     /locus_tag="N7C74_004576"
     CDS             complement(133..429)
                     /gene="yciI"
                     /locus_tag="N7C74_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HGU2434287.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            653..1372
                     /gene="tonB"
                     /locus_tag="N7C74_004577"
     CDS             653..1372
                     /gene="tonB"
                     /locus_tag="N7C74_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HGU2434288.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQQKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(1412..1810)
                     /gene="yciA"
                     /locus_tag="N7C74_004578"
     CDS             complement(1412..1810)
                     /gene="yciA"
                     /locus_tag="N7C74_004578"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HGU2434289.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATDALFKYVAVDPEGKPRALPVE"
     gene            complement(1915..2454)
                     /gene="yciB"
                     /locus_tag="N7C74_004579"
     CDS             complement(1915..2454)
                     /gene="yciB"
                     /locus_tag="N7C74_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HGU2434290.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2484..3227)
                     /gene="yciC"
                     /locus_tag="N7C74_004580"
     CDS             complement(2484..3227)
                     /gene="yciC"
                     /locus_tag="N7C74_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HGU2434291.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASIRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(3255..3344)
                     /locus_tag="N7C74_004581"
                     /pseudo
     CDS             complement(3255..3344)
                     /locus_tag="N7C74_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
BASE COUNT         1017 a          856 c          824 g          793 t
ORIGIN      
        1 ctcacatcat ataaagaaat acagataaag ttattatctg cttgtggtgg tgaatgcaat
       61 gaccgaccta taaggaaagg ccaaacaaga acacggttgc aaaaaccgtg cccttaaata
      121 ttggatctct actcagaaca ctttcttaaa tggtttcact gaaacgtgtt catagactcc
      181 tgccgctacg tacgggtcag catcggccca ggcctgagct gcttccagcg attcaaattc
      241 agcaataacg gttgagccag taaatcccgc agcccctgga tcgttactgt ctaccgctgg
      301 cattggacca gctgtcaaca aacgaccttc atcatgcagt aactgtaaac gtgctaaatg
      361 tgccggacga acggaaaggc gtttttcgag gctatcagct ttgtcttgag cgtaaataac
      421 atacaacacg ggcacaactc cttgttcggg aaagttgtaa agtacgttat ttgaaagggc
      481 gaagatctgc aacggaaaga tgatgtcttt gttaagacca tgcataaagt aaggtcaatt
      541 acgccaaaaa tgacattttc actgatcctg atcgtcttgc cttattgaat atgattgcta
      601 tttgcattta aaatcgagac ctggtttttc tactgaaatg attatgactt caatgaccct
      661 tgatttacct cgccgcttcc cctggccgac gttactttcg gtctgcattc atggtgctgt
      721 tgtggcgggt ctgctctata cctcggtaca tcaggttatt gaactacctg cgcctgcgca
      781 gccgatttct gtcacgatgg ttgcgcctgc tgatctcgaa ccgccacaag ccgttcagcc
      841 gccaccggag ccggtggtag agccagaacc ggaacctgag ccgatccccg aaccgccaaa
      901 agaagcaccg gtggtcattg aaaagccgaa gccgaaacct aagccaaaac cgaagccggt
      961 gaaaaaggta caggagcagc aaaaacgcga tgtcaaaccc gtagagtcgc gtccggcatc
     1021 accgtttgaa aatacggcac cggcacgccc gacatcaagt acagcaacgg ctgcaaccag
     1081 caagccggtt accagtgtgg cttcaggacc acgcgcatta agccgtaatc agccgcagta
     1141 tccggcacga gcacaggcat tgcgcattga agggcaggtt aaagttaaat ttgacgtcac
     1201 gccggatggt cgcgtggata acgtacaaat cctctcagcc aagcctgcga acatgtttga
     1261 gcgtgaggtg aaaaatgcga tgcgcagatg gcgttatgag ccgggtaagc caggcagtgg
     1321 gattgtggtg aatatcctgt ttaaaattaa cggcaccacc gaaattcagt aagcagaaag
     1381 tcaaaagcct ccgaccggag gcttttgact attactcaac aggtaaggcg cgaggttttc
     1441 cttcaggatc aaccgcgaca tacttaaata atgcgtctgt cgctttatag cgttgcccga
     1501 ttggttcaga cgctactttt ttcacccaca cttcaatatt aatgctgacc gatgtcgtcc
     1561 ctttctggac acagcgtgca tagcagcaca ccacatcgcc gaccgcaacc ggccgtaaga
     1621 aagtcattcc ttcaacccgc acagtcacta cgcgaccgtg ggcaatttct tttgccagaa
     1681 tagcgccgcc aatatccatt tgtgacatta accaaccacc aaagatgtca ccattggcat
     1741 tggtatcggc gggcatggct aaagtacgta aaacaagatc gccctgaggg acgttatgtg
     1801 ttgtagacat ggtaaaaccg acttaaaagg aaaatcacag gcgcgatgct actatgattt
     1861 tcctgaggag aacagaggag atacagcgcc agccccaaag gactggcagt ctggttagga
     1921 tttatcttcc tgcggcatgt ggcggtagat atagataccg cttaacaatg tgaagattaa
     1981 ggtaagggcg gtcaggccaa agactttaaa gttgacccaa atattttgcg gcagccagaa
     2041 tgcgatgtag atgtttgcca gaccgcaaag gataaagaaa acagcccagg ccagattcag
     2101 cttcgaccat accggttgcg gcagcgtgag ttctttaccc agcatccgct gaattagcgg
     2161 ctttttcatc acccattggc tgactaacag ggctcccgca aacagagcat aaatgactgt
     2221 aaccttccat ttaataaact catcattgtg gaagaacagc gtcaagccac cgaagacgac
     2281 caccagaaca aaagtgatca gggccatctt ctcaacctta cgaaagcgaa cccagctata
     2341 gataagcaca atcgccgtgg cgacgatcag cgccgcagta gccgcataga tgtcataaat
     2401 tttgtaaaac gcgaaaaaga caaccagcgg taaaaaatca agaaactgct tcattttacg
     2461 attccgtaat caaatgctta aggttattgg cgaatcaaca tatacaggcg gaacagatag
     2521 atgagcaata tggctgaaat caggttgctc aaggtgttcg ccagtacggc accaatttcc
     2581 ggggttaatg cggcaaaaga agaggcaaaa agcagcaaca gtgtttttgc cagcaaccag
     2641 ctcagtactg cgggtgccac cagacgcata ttcgcccaag tcagccgcat actgctacgg
     2701 atcgaggcaa aaacgcccat tttgtcctgt accagcatca ccggtgccag ggccagtaat
     2761 atggccatga taattcccgg aacgaccacc agcatgatgc caatctgtac taaaagggta
     2821 gtcagaaaaa tcagaataaa taactttggc aatatcggcg cactggcacc aatagctcgc
     2881 agcgcactga ctctctgacc cgcagacacc agctggataa ttaatattac gcctccggcg
     2941 agaatggcgt taccgattaa tcctgaaaaa gtggacgccg ctgaagcctg cagcaaaatt
     3001 tgttgctgtt ccggtgacat attctgaacc aggtcgaaca acccactact gccgctaacg
     3061 ggcacgccgt cattgagctg cgcaagctgt gcatcactgg gtgagaaaac atgccctaac
     3121 accactgtga taaacgcaca tagcaacgat accaacagaa tggtcataaa ttgattacgg
     3181 aagaaatttc cggtgtcacg gtatacggac tgcgccgtga tagacatgca ctctccttga
     3241 gttttatgca ggtgttaatt agcgggcaat tgtaccctgg ttaagcctcg ggtggcagca
     3301 tcaggtcttg tatggaaaag catatctttg taaagcggag gtaattgctg gccttcaggc
     3361 gtgggcaaac cgcggcaagt ggcgaacgag ttaatatttg cgtagcgaaa atatttaaaa
     3421 attgatttaa atcacgttaa ccaggattct caatgcaact tctaaattaa tccagatcaa
     3481 taaatggtga
//
LOCUS       DBFIFM010000304         3478 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.305, whole genome shotgun sequence.
ACCESSION   DBFIFM010000304 DBFIFM010000000
VERSION     DBFIFM010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.305"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            222..1154
                     /gene="rfaD"
                     /locus_tag="N7C74_004582"
     CDS             222..1154
                     /gene="rfaD"
                     /locus_tag="N7C74_004582"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HGU2434292.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1164..2210
                     /gene="rfaF"
                     /locus_tag="N7C74_004583"
     CDS             1164..2210
                     /gene="rfaF"
                     /locus_tag="N7C74_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HGU2434293.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLKELNALLLQEEA"
     gene            2214..3206
                     /gene="rfaC"
                     /locus_tag="N7C74_004584"
     CDS             2214..3206
                     /gene="rfaC"
                     /locus_tag="N7C74_004584"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HGU2434294.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPEIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGENQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3203..>3478
                     /locus_tag="N7C74_004585"
     CDS             3203..>3478
                     /locus_tag="N7C74_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HGU2434295.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGA"
BASE COUNT          881 a          846 c          891 g          860 t
ORIGIN      
        1 cttccgttat ttatcataca tcggaatatt gatactaaag cactattcac atgcaaaacc
       61 aacatccgcc atgaaggact agctaaaacc caaactagtt tgttgcaatt agcatccttg
      121 cacctctatg taaagggctg aagggattcg gatgtgatgg tatgattaca gacattcgtg
      181 tctgagattg tctctgactc cataattcga aggttacagt tatgatcatc gttaccggcg
      241 gcgcgggctt tatcggcagc aacatcgtta aagccctgaa tgataaaggc atcaccgata
      301 ttctggtggt ggacaacctg aaagacggca ccaagtttgt gaacctggtg gatctgaata
      361 tcgcagacta tatggataag gaagacttcc tgatccagat tatggctggc gaagagttcg
      421 gcgatgtcga agcgattttc cacgaaggcg cgtgctcttc caccaccgag tgggacggca
      481 agtatatgat ggataacaac tatcaatact ccaaggagct gctgcactac tgcctggagc
      541 gtgaaatccc gttcctgtac gcttcttccg cagccaccta cggcggacgc acctccgact
      601 ttattgaatc ccgcgagtac gaaaaaccgt tgaacgtcta cggttactca aaattcctgt
      661 ttgatgaata tgttcgccaa atcctgccgg aagcgaactc gcagattgtt ggcttccgct
      721 atttcaacgt ttatggaccg cgtgaaggcc ataaaggcag catggcgagc gtcgctttcc
      781 atctcaacac ccagcttaat aacggtgaat caccgaagct gttcgaaggt agcgagaact
      841 ttaaacgcga cttcgtctat gttggcgatg tggcagatgt aaacctgtgg ttcctggaaa
      901 atggcgtttc cggcatcttc aacctcggca ccggtcgtgc ggaatccttc caggctgtag
      961 ctgatgctac gctggcttat cacaagaaag gccagatcga atacattccg ttcccggata
     1021 agctgaaagg ccgctaccag gcgttcactc aggcagatct gacaaatctg cgcgcggcgg
     1081 gttacgacaa accgttcaaa accgttgctg aaggtgtaac ggaatacatg gcctggctga
     1141 atcgcgacgc ataagagctc tgcatgaaaa tactggtgat cggcccgtct tgggttggcg
     1201 acatgatgat gtcgcaaagt ctctatcgca cgctccaggc gcgctatccc caggcgataa
     1261 tcgatgtgat ggcaccggca tggtgccgtc cattattatc gcggatgccg gaagttaacg
     1321 aagctattcc tatgcctctc ggtcacggag cgctggaaat cggcgaacgc cgcaaactgg
     1381 gtcatagtct gcgtgaaaag cgctacgacc gcgcctacgt cttacctaac tccttcaaat
     1441 ccgcattagt gcctttcttc gcgggtattc ctcatcgcac cggctggcgc ggcgagatgc
     1501 gctacggttt actcaacgat gtacgcgtgc tcgataaaga agcctggccg ctaatggtgg
     1561 aacgctatgt cgcgctggcc tatgacaaag gcattatgcg cacagcacaa gatctgccgc
     1621 agccattgtt atggccgcag ttgcaggtga gcgaaggtga aaaatcatat acctgtaatc
     1681 aattttcgct ttcatcagaa cgtccgatga ttggtttttg cccgggtgcg gagtttggtc
     1741 cggcaaaacg ctggccacac taccactatg cggagctggc aaagcagctg attgatgaag
     1801 gttatcaggt ggttctgttt ggctctgcga aagatcatga agcaggcaat gagattcttg
     1861 ccgctttgaa taccgagcag caggcatggt gtcggaacct ggcgggggaa acacagcttg
     1921 atcaagcggt tatcctgatt gcagcctgta aagccattgt cactaacgat tctggcctga
     1981 tgcacgttgc ggcggcgctc aatcgtccgc tggttgccct gtatggtccg agtagcccgg
     2041 acttcacacc gccgctatcc cataaagcgc gcgtgatccg cctgattacc ggctatcaca
     2101 aagtgcgtaa aggtgacgct gcggagggtt atcaccagag cttgatcgac attactcccc
     2161 agcgcgtact gaaagaactc aacgcgctat tgttacaaga ggaagcctga cggatgcggg
     2221 ttttgatcgt taaaacatcg tcgatgggcg atgttctcca tacgttgccc gcactcactg
     2281 atgcccagca ggcaatccca gaaattaagt ttgactgggt ggtggaagaa gggttcgcac
     2341 agattccttc ctggcacgct gccgttgagc gagttattcc tgtggcaata cgtcgctggc
     2401 gtaaagcctg gttctcggcc cctataaaag ctgaacgcaa agcgtttcgt gaagcgctac
     2461 aagcagagaa ctatgacgca gttatcgacg ctcaggggct ggtaaaaagc gcggcgctgg
     2521 ttacgcgtct ggcacatggc gtaaagcatg gcatggactg gcaaaccgct cgcgaacctt
     2581 tagccagcct gttttacaat cgtaagcatc atattgcaaa acagcagcac gccgtagaac
     2641 gcacccgcga actgtttgcc aaaagtttgg gctatagcaa accgcaaacc cagggcgatt
     2701 atgctatcgc acagcatttt ctgacgaacc tgcctacaga tgctggcgaa tatgccgtat
     2761 ttcttcatgc gacgacccgt gatgataaac actggccgga agaacactgg cgagaattga
     2821 ttggtttact ggctgattca ggaatacgga ttaaacttcc gtggggcgcg ccgcatgagg
     2881 aagaacgggc gaaacgactg gcggaaggat ttgcttatgt tgaagtattg ccgaagatga
     2941 gtctggaagg cgttgcccgc gtgctggccg gggctaaatt tgtagtgtcg gtggatacgg
     3001 ggttaagcca tttaacggcg gcactggata gacccaatat cacggtttat ggaccaaccg
     3061 atccgggatt aattggtggg tatggggaga atcaagtaga gtgtcgttct acaagtatgt
     3121 ctcttgcaga tttgccagct caaaccgttt ttcaaaattt aaatcttgaa ataataacca
     3181 ataagttgac atcggagata agatgacctc aacattattt ttctctctcg agaaaaaaaa
     3241 ctggatagcg tactggaaca gagctctcgt attcttattc attaccacct attttttggg
     3301 tgggattaca aggtataaac atcttattgt tattcttatg acaataacga caatcgtcta
     3361 tctctgcaaa cggccaaaac actatctctc actatttaaa acatttcttt ttggtagtgt
     3421 tgccatatta actattgctg cattgctgtc acttcttcaa tcccctgatg caggtgct
//
LOCUS       DBFIFM010000305         3444 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.306, whole genome shotgun sequence.
ACCESSION   DBFIFM010000305 DBFIFM010000000
VERSION     DBFIFM010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.306"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..153)
                     /locus_tag="N7C74_004586"
     CDS             complement(<1..153)
                     /locus_tag="N7C74_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HGU2434296.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRAL"
     gene            467..1783
                     /gene="shiA"
                     /locus_tag="N7C74_004587"
     CDS             467..1783
                     /gene="shiA"
                     /locus_tag="N7C74_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HGU2434297.1"
                     /translation="MDSTLISTRPDERTVSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQYNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            1885..3339
                     /gene="amn"
                     /locus_tag="N7C74_004588"
     CDS             1885..3339
                     /gene="amn"
                     /locus_tag="N7C74_004588"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HGU2434298.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          816 a          834 c          826 g          968 t
ORIGIN      
        1 aagcgcccga ataacaggaa agatggccat agcatcgcca atctgatcgg gaatatggat
       61 gacaacaaag tctgtttttt caatattgaa attataagct ttataatcgt agtaactaaa
      121 tgcaatacgt ctcaacaatg atactaaaaa cataactaac ctcgcctccc tactggttat
      181 aatgcaatgc agtctatcag aatcatcagg gtgcaatttt gtgcatatgc ggacttttat
      241 gtttcatatc tcgaacctgt ggggcctctg cttaatcctt aaacaacacc agcaactcct
      301 gcgctttcat cttccagcga atttttcatg ttgccgttaa tcagccataa aatcatttgc
      361 agatgcgctc tgtcgaggta gtctcataag gttcgtttat agatcgacgc aatgtgagtt
      421 accttttcca tactaattat aaaaagacag tacaaacagg atcattatgg actccacgct
      481 catctccact cgtcccgatg aaaggacggt ttcgttaagt cgcgcccgac gagctgcgtt
      541 aggcagtttc gctggtgccg tcgtcgactg gtatgatttt ttactctatg gcattaccgc
      601 cgcactggtg tttaatcgcg agtttttccc gcaagtaagc ccggctatgg gaacgctcgc
      661 cgcatttgcc acctttggcg tcggattcct tttccgtccg ctcggcggtg tcattttcgg
      721 tcactttggc gaccgactgg gacgtaagcg catgttaatg ctgaccgtct ggatgatggg
      781 catcgcgaca gccttgattg gtattcttcc ttcattctcg accattgggt ggtgggcacc
      841 tattttactg gtgacgctgc gtgccattca gggatttgcc gtcggcggcg aatggggagg
      901 cgcggcgttg ctttccgttg aaagtgcacc gaaaaataaa aaagcctttt acagtagcgg
      961 tgtacaagtt ggctacggtg taggtttact gctttcaacc ggactggttt cattgatcag
     1021 tatgatgacg actgacgaac agtttttaag ctggggctgg cgcattcctt tcctgtttag
     1081 catcgtactg gtactgggag cattgtgggt gcgcaatggc atggaagagt ccgcggaatt
     1141 tgaacaacag caatataatc aagcggccgc gaaaaaacgc atcccggtta tcgaagcgct
     1201 gttacgacat cccggtgctt tcctgaagat tattgcgcta cgactgtgcg agttgctgac
     1261 aatgtacatc gttaccgcct ttgcacttaa ttattcaact cagaatatgg ggttaccgcg
     1321 cgaacttttc cttaatattg gtttgctggt aggtggatta agctgcctga caattccctg
     1381 ttttgcctgg cttgccgatc gttttggtcg ccgcagggtt tatatcacag gcgcgttgat
     1441 cggaacgttg agcgcatttc ctttctttat ggcgcttgaa gcacaatcta ttttctggat
     1501 agttttcttc tccataatgc tggcaaacat tgcgcatgac atggtggtgt gtgtgcaaca
     1561 accgatgttt accgaaatgt ttggtgccag ttatcgctat agtggtgctg gagtcggtta
     1621 tcaggttgcc agtgtggttg gcggtggatt tacacctttt attgccgctg cgctcatcac
     1681 ttactttgcc gggaactggc atagcgtcgc catttatttg ctggctggat gtctgatttc
     1741 cgcaatgacc gctttgttga tgaaagacaa tcaacgcgct tgatagtctg gcgaagatca
     1801 tccgatcttc gccttacact tttgtttcac attcctgtga catactatcg gatgtgcggt
     1861 aattgtatgg aacaggagac acacatgaat aataagggct ccggtctgac cccagctcag
     1921 gcactggata aactcgacgc gctgtatgag caatctgtag tcgcattacg caacgccatt
     1981 ggcaactata ttacaagtgg cgaattacct gatgaaaacg cccgcaaaca aggtcttttt
     2041 gtctatccat ctctgaccgt aacctgggac ggtagcacaa caaatccccc caaaacgcgc
     2101 gcatttggtc gttttactca cgcaggcagc tacaccacca cgattactcg ccctactctc
     2161 tttcgttcgt atcttaatga acaacttacg ttgctgtatc aggattatgg cgcgcatatc
     2221 tcggtgcaac cctcacagca tgaaatccct tatccttatg tcatcgatgg ctctgaatta
     2281 acacttgatc gctcaatgag cgctgggtta actcgctatt tcccgacaac agaactggcg
     2341 caaattggcg atgaaactgc agacggcatt tatcatccaa ctgaattctc cccgctatcg
     2401 cattttgatg cgcgccgcgt cgatttttcc ctcgcgcggt tgcgccatta taccggtacg
     2461 ccagttgaac attttcagcc gttcgtcttg tttaccaact acacacgtta tgtggatgaa
     2521 ttcgttcgtt ggggatgcag ccagatcctc gatcctgata gtccctacat tgccctttct
     2581 tgtgctggcg ggaactggat caccgccgaa accgaagcgc cagaagaagc catttccgac
     2641 cttgcatgga aaaaacacca gatgccagca tggcatttaa ttaccgccga tggtcagggt
     2701 attactctgg tgaatattgg cgtgggaccg tcaaatgcta aaaccatctg cgatcatctg
     2761 gcagtgctac gcccggatgt ctggttgatg attggtcact gtggcggatt acgtgaaagt
     2821 caggccattg gcgattatgt acttgcacac gcttatttac gcgatgacca cgttcttgat
     2881 gcggttctgc cacccgatat tcctattccg agcattgctg aagtgcaacg tgcgctttat
     2941 gacgccacca agctggtgag cggcaggccc ggtgaggaag tcaaacagcg gctacgtact
     3001 ggtactgtgg taaccacaga tgacaggaac tgggaattac gttactcagc ttctgcactt
     3061 cgttttaact taagccgggc cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa
     3121 ggatatcgtt tccgcgtgcc atacgggaca ctactgtgtg tttcagataa accgttgcat
     3181 ggcgagatta aacttcccgg tcaggctaac cgtttttatg aaggcgctat ttccgaacac
     3241 ctacaaattg gcattcgggc gatcgatttg ctgcgcgcag aaggcgaccg actgcattca
     3301 cgtaaattac gaacctttaa tgagccgccg ttccgataat aagttctgaa ccttcttatc
     3361 aaaaatagat gtgaaggatt aagtaagaca tctggcaaat agcccgcaat aaacgcgggc
     3421 tgtttgcttc cttgggcgga tacg
//
LOCUS       DBFIFM010000306         3425 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.307, whole genome shotgun sequence.
ACCESSION   DBFIFM010000306 DBFIFM010000000
VERSION     DBFIFM010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.307"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..2461
                     /locus_tag="N7C74_004589"
     CDS             <1..2461
                     /locus_tag="N7C74_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGU2434299.1"
                     /translation="VLFAEAFITTPYVFVMQKAPDSEQTLKKGMKVAIPYYYELHSQL
                     KEMYPEVEWIKVDNASAAFHKVKEGELDALVATQLNSRYMIDHYYPNELYHFLIPGVP
                     NASLSFAFPRGEPELKDIINKALNAIPPSEVLRLTEKWIKMPNVTIDTWDLYSEQFYI
                     VTTLSVLLVGSSLLWGFYLLRSVRRRKVIQGDLENQISFRKALSDSLPNPTYVVNWQG
                     NVISHNSAFEHYFTADYYKNAMLPLENSESPFKDVFSNTHEVTAETKENRTIYTQVFE
                     IDNGIEKRCINHWHTLCNLPASEHAVYICGWQDITETRDLIHALEVERNKAINATVAK
                     SQFLATMSHEIRTPISSIMGFLELLSGSGLSKEQRVEAISLAYATGQSLLGLIGEILD
                     VDKIESGNYQLQPQWVDIPTLVQNTCHSFGAIAASKSIALSCSSTFPDHYLVKIDPQA
                     FKQVLSNLLSNALKFTTEGAVKITTSLVHIDDNHAVIKMTIMDSGSGLSQEEQQQLFK
                     RYSQTSAGRQQTGSGLGLMICKELIKNMQGDLSLESHPGIGTTFTITIPVEIIQQVAA
                     VEAKAEQPITLPEKLSILIADDHPTNRLLLKRQLNLLGYDVDEATDGVQALHKVSMQH
                     YDLLITDVNMPNVDGFELTRKLREQNSSLPIWGLTANAQANEREKGLNCGMNLCLFKP
                     LTLDVLKTHLSQLHQVAHIVPQYRHLDIEALKNNTANDLQLMQEILMTFQHETHKDLP
                     AAFHALEAGDNRTFHQCIHRIHGAANILNLQKLINISHQLEITPVSDDSKPEILQLLN
                     SVKEHIAELDQEIAVFCQQNN"
     gene            complement(2517..>3425)
                     /gene="yfdE"
                     /locus_tag="N7C74_004590"
     CDS             complement(2517..>3425)
                     /gene="yfdE"
                     /locus_tag="N7C74_004590"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HGU2434300.1"
                     /translation="NDHDKSIFINMLKQADVLAENFRPGTMEKLGLSWERLQEINPRL
                     IYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYPDAPPVRVGTSLADLCGGVYLF
                     SGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMAYIATGKSPQRLGNRHPYMAPF
                     DVFDTQDKPITICCGNDKLFSALCQALELTELVNDPRFSSNILRVQNQTILKQYIERT
                     LKTQAAEVWLARIHEVGVPVAPLLSVAEAINLPQTQARNMLIEAGGIMMPGNPIKISG
                     CADPHVMPGAATLDQHGEQIRQEFSS"
BASE COUNT         1027 a          768 c          699 g          931 t
ORIGIN      
        1 tgttttattt gctgaagcct tcataacaac gccttacgtt tttgtcatgc aaaaagcgcc
       61 tgacagtgaa caaacattaa aaaaaggaat gaaagttgcc attccatatt attatgagct
      121 tcattcgcaa ttaaaagaga tgtatccgga ggtagagtgg ataaaagtcg ataacgccag
      181 cgctgcattt cacaaggtca aggaaggcga acttgatgct ctggtcgcga cacagttaaa
      241 ttcacgttac atgatcgacc attactatcc taatgaactg tatcattttc ttattcccgg
      301 cgttccgaat gcatcgcttt cgttcgcttt tcctcgcgga gaaccggaac ttaaggatat
      361 tattaataaa gcactgaatg caatcccccc aagcgaagtt ctgcgcctga ccgaaaaatg
      421 gattaaaatg cccaatgtga ccattgacac atgggacctc tatagcgagc aattttatat
      481 tgttacgaca ttatccgttt tattagttgg cagtagcctt ttatggggat tctacctgtt
      541 acgctcagtt cgtcgtcgta aagtcattca gggtgattta gaaaaccaaa tatcattccg
      601 gaaagcgctc tcggactcct taccgaatcc aacttatgtt gtaaactggc aaggtaatgt
      661 cattagtcac aatagtgcat ttgaacatta tttcactgct gattactaca aaaatgcaat
      721 gttgccatta gaaaacagtg aatcaccctt taaagatgtt ttttctaata cgcatgaagt
      781 cacagcagag acgaaagaaa accgaacaat atacacacag gtatttgaaa ttgataatgg
      841 catcgagaaa agatgcatta atcactggca tacattatgt aatctgccag ccagcgaaca
      901 tgctgtttat atttgtggtt ggcaagatat taccgagacg cgtgatttaa ttcatgcact
      961 cgaagtagag agaaataaag cgatcaatgc aactgtcgca aaaagtcagt ttctggcaac
     1021 aatgagtcac gaaataagaa caccaataag ctccattatg ggcttcctgg aacttctgtc
     1081 gggttctggt cttagcaagg agcaacgggt ggaggcgatt tcactagcct acgccaccgg
     1141 acaatcactc ctcggcttaa ttggtgaaat ccttgatgtc gacaaaattg aatcgggtaa
     1201 ctatcaactt caaccacaat gggtcgatat ccctacttta gtccagaaca cttgtcactc
     1261 tttcggtgcg attgctgcaa gcaaatcgat cgcattaagt tgcagcagta catttcctga
     1321 tcattacctg gttaagatcg accctcaggc gtttaagcag gtcttatcaa atttactgag
     1381 taatgctctc aaatttacca ccgagggggc agtaaaaatt acgacctccc tggttcacat
     1441 tgatgacaac cacgctgtaa tcaaaatgac gattatggat tctggaagtg gattatcaca
     1501 ggaagaacaa caacaactgt ttaaacgtta tagccaaaca agtgcaggtc gtcagcaaac
     1561 aggttctggt ttaggcttaa tgatctgcaa agaattaatt aaaaacatgc agggtgattt
     1621 gtcattagaa agtcatccag gcataggaac aacatttacg atcacaatcc cggtagaaat
     1681 tatccaacaa gtggcggctg tcgaggcaaa agcagaacaa cccatcacac tacctgaaaa
     1741 gttgagcata ttaatcgcgg atgatcatcc gaccaacagg ctattactca aacgccagct
     1801 aaatctatta ggatatgatg ttgatgaagc cactgatggt gtgcaagcgc tacacaaagt
     1861 cagtatgcaa cattatgatc tgcttattac tgacgttaat atgccgaatg tggatggttt
     1921 tgagttgact cgcaaactcc gtgagcaaaa ttcgtcctta cccatctggg ggcttacagc
     1981 caacgcacag gctaacgaac gtgaaaaagg gttaaattgc ggcatgaact tatgtttgtt
     2041 caaaccgttg accctggatg tactgaaaac acatttaagt cagttacacc aggttgcgca
     2101 tattgtacct cagtatcgcc accttgatat cgaggccctg aaaaataata cggcgaacga
     2161 tctacaactg atgcaggaga ttctcatgac tttccagcat gaaacacata aagatttacc
     2221 cgctgcgttt catgcactag aagctggcga taatagaact ttccatcagt gtattcatcg
     2281 catccacggt gcggctaaca tcctgaattt gcaaaagttg attaatatta gccatcagtt
     2341 agaaataaca cctgtttcag atgacagtaa gcctgaaatt cttcagttgc taaactctgt
     2401 aaaagaacac attgcagagc tagaccagga aattgctgtt ttctgtcaac aaaataacta
     2461 aatagtggct cccacaatgt tcaaatgtgg gagctattta ccagcacatc ttacatttat
     2521 gatgagaact cctggcgaat ttgctcccca tgctggtcga gcgttgccgc tcccggcata
     2581 acatgcgggt ccgcgcagcc actgattttt atcggattac ccggcatcat tattcccccg
     2641 gcttcaatca acatatttct cgcctgagtt tgtggcaaat taatggcctc agccacactt
     2701 aataacggcg cgacgggcac accaacttca tgtattctgg ctaaccaaac ttcagctgcc
     2761 tgtgttttta acgtccgctc aatatattgt ttaagaatag tctggttttg tacgcgtaaa
     2821 atattgctgc taaatctggg atcattaacc agttccgtaa gctccagtgc ctggcataac
     2881 gcagagaaaa gcttgtcatt accacaacaa atcgtaatcg gcttatcctg agtatcgaaa
     2941 acatcaaaag gtgccatgta ggggtggcga tttcccaggc gttgtggtga cttccctgtc
     3001 gcaatatatg ccatcagacc atgctccaga aaactcagcg tggcatcaaa catcgctata
     3061 tcgacatgcg cccctctttg gctcttttcg cggccataaa gtgcactcac tattccgctg
     3121 aataaataaa caccgccgca tagatccgca agagaggtac caacacgcac tggcggagca
     3181 tcagggtacc ctgtttccat cattatcccg ctcattgcct gaatgatggt atcgtaggca
     3241 ggagcatctt ttagcggacc ggtatgtccg aaacctgacg atgaagcata tatgaggcgc
     3301 gggttgattt cttgtaacct ttcccatgaa agccccagtt tttccattgt acctgggcga
     3361 aaattctcag ctaatacatc agcttgttta agcatattta taaatatact tttatcgtga
     3421 tcatt
//
LOCUS       DBFIFM010000307         3390 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.308, whole genome shotgun sequence.
ACCESSION   DBFIFM010000307 DBFIFM010000000
VERSION     DBFIFM010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.308"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..528)
                     /locus_tag="N7C74_004591"
     CDS             complement(<1..528)
                     /locus_tag="N7C74_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HGU2434301.1"
                     /translation="MAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAEKAAEKKRRREEQKQKDKLKIQKLALKPRSYWIKQAQQAVHAFIRERDRDL
                     PCISCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELIS
                     RIGQEAVEEIESNHNR"
     gene            complement(521..691)
                     /locus_tag="N7C74_004592"
     CDS             complement(521..691)
                     /locus_tag="N7C74_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HGU2434302.1"
                     /translation="MIDQNRSYEQESVERALTCANCGQKLHVLEVHVCEHCCAELMSD
                     SNSSMHEEKDDG"
     gene            complement(688..870)
                     /locus_tag="N7C74_004593"
     CDS             complement(688..870)
                     /locus_tag="N7C74_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGU2434303.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHCARKTR"
     gene            900..1571
                     /gene="bet"
                     /locus_tag="N7C74_004594"
                     /pseudo
     CDS             900..1571
                     /gene="bet"
                     /locus_tag="N7C74_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
     gene            1568..2248
                     /locus_tag="N7C74_004595"
     CDS             1568..2248
                     /locus_tag="N7C74_004595"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HGU2434304.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMAGFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            2245..2427
                     /locus_tag="N7C74_004596"
     CDS             2245..2427
                     /locus_tag="N7C74_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HGU2434305.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKREAICTKHLLLS"
     gene            2400..2591
                     /locus_tag="N7C74_004597"
     CDS             2400..2591
                     /locus_tag="N7C74_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HGU2434306.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDLV"
     gene            2668..2883
                     /locus_tag="N7C74_004598"
     CDS             2668..2883
                     /locus_tag="N7C74_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGU2434307.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQPFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            2982..3203
                     /locus_tag="N7C74_004599"
     CDS             2982..3203
                     /locus_tag="N7C74_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HGU2434308.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
BASE COUNT          866 a          812 c          857 g          855 t
ORIGIN      
        1 gcggttatgg tttgattcga tttcctctac tgcttcctgc ccgatgcggc taatcagttc
       61 gacgcgatac ggaacgagat ttccgctttt gtgctggttg cacaccacgc attgcttgtg
      121 aatattgcgt tcatcaaatc ggagttgagg tgccgcagca gttgtccggt aatgtccggc
      181 atcccactga gcagacgtga gcgttccgca cgagatacat ggtaagtcgc ggtctctttc
      241 tctgatgaag gcgtgtacgg cttgttgggc ttgtttaatc cagtaactgc ggggctttaa
      301 ggcgagtttt tgaatcttaa gtttatcttt ctgtttctgc tcctctcgtc gtcgtttctt
      361 ctctgctgcc ttttctgcct tttccgcttt ttcgcgttct ttgcttcgtc gttcgagtgc
      421 tatcttggtt ccacactctg gagagcacca ccactgatta gcgaatgcag ggtgaaacca
      481 ttcccgacat tcatcgtttt tacatcgtct tcgcgctggt ttagccatca tctttttcct
      541 cgtgcatcga gctattcgaa tcgctcatca gctctgcgca gcagtgctca cacacgtgaa
      601 cttccagcac atgcagcttc tgaccgcagt tagcgcacgt taaagcccgc tcgacgcttt
      661 cttgttcgta acttcgattt tggtcaatca ccttgttttc ctcgcacaat gtcttagcca
      721 ccggatatcc cacaggtgag ccgtgtagtt gaaggttttt acgtcagatt cttttgggat
      781 tggcttgcgt ttatttctgg agcgtttcgt tggaaggtat ttgcagtttt cgcagatgat
      841 gtcggtgaaa cttcgtcgct gtcgtctcat gccgccctgt ctccccatcg cgctttcatg
      901 ccagcgatgc gcagttcatc gcattgttga tcgtcgccaa ccagtacggc cttaatccgt
      961 ggacgaaaga aatttacgcc ttccctgata agcagaacgg catcgttccg gtggtgggcg
     1021 ttgatggctg gtcccgcatc atcaatgaaa accagcagtt tgatggcatg gactttgagc
     1081 aggacaatga atcctgtaca tgccggattt accgcaagga ccgtaatcat ccgatctgcg
     1141 ttaccgaatg gatggatgaa tgccgccgcg aaccattcaa aacccgcgaa ggcagagaaa
     1201 tcacggggcc gtggcagtcg catcccaaac ggatgttacg gcataaagct atgattcagt
     1261 gtgcccgtct ggccttcgga tttgctggta tctatgacaa ggatgaagcc gagcgcattg
     1321 tcgaaaatac tgcatacact gcagaacgcc agccggaacg cgacatcact ccggttaacg
     1381 atgaaaccat gcaggagatt aacactctgc taatcgccct ggataaaaca tgggatgacg
     1441 acttattgcc gctctgttcc cagatatttc gccgcgacat tcgcgcatcg tcagaactga
     1501 cacaggccga agcagtgaaa gctcttggat tcctgaaaca gaaagccact gagcagaagg
     1561 tggcagcatg acaccggaca ttatcctgca gcgtaccggg atcgacgtga gagctgtcga
     1621 acagggggat gatgcatggc acaaattacg gctcggcgtc atcaccgctt cagaagttca
     1681 caacgtgata gcaaagcccc gctcaggaaa gaagtggcct gacatgaaaa tgtcctactt
     1741 ccacaccctg ctggctgagg tttgcaccgg tgtggctccg gaagttaatg ctaaggcgct
     1801 ggcctgggga aaacagtacg agaacgacgc cagaaccctg tttgaattca cttccggcgt
     1861 gaatgttact gaatccccga tcatctatcg cgacgaaagt atgcgcaccg cctgctctcc
     1921 cgatggttta tgcagtgacg gcaacggcct tgagctgaaa tgcccgttta cctcccggga
     1981 tttcatgaag ttcaggctcg gtggtttcga ggccataaag tcggcttaca tggcccaggt
     2041 gcagtacagc atgtgggtga cgcgaaaaga tgcctggtac tttgccaact atgacccgcg
     2101 tatgaagcgt gaaggcctgc attatgtcgt ggttgagcgg gatgaaaagt acatggcggg
     2161 ttttgacgag atggtgccgg agttcatcga aaaaatggac gaggcactgg ctgaaattgg
     2221 ttttgtattt ggggagcaat ggcgatgaag catcctcacg ataatatccg cgtaggcacg
     2281 atcactttcg tctactccgt tacgaagcga ggctgggtat ttcccggcct ttctgttatc
     2341 cgaaatccgc tgaaagccca gcggctggct gaggagataa ataataaacg ggaggcgata
     2401 tgcacaaagc atctcctgtt gagttaagaa cgagtattga gatggcacat agccttgctc
     2461 aaattggagt caggtttgtg ccaataccag tagaaacaga cgaagaattt catacgttag
     2521 ccacatccct ttcgcaaaag ctggaaatga tggtggcgaa agcagaagca gatgagagag
     2581 acctggtatg acaaccactg aatgcatttt tctggcagcg ggcttcatat tctgtgtgct
     2641 tatgcttgcc gacatgggac ttgttcaatg acacctcagc aagaaaacgc ccttcgcagt
     2701 attgcccgtc aggctaattc tgaaatcaaa aaagccagac agccgtttcc ggataaaaac
     2761 gtcgatgaca tttgccgtag cgtactgaag aagcaccgcg aaacggtaac gctgatggga
     2821 ttcacaccga ctcacttaag tctggcaatc ggcatgttaa acggcgtctt taaggaacgg
     2881 tgaacatgaa aagcaaaatc atcagggagc tacaggctcc ttttttatta ttcgcattca
     2941 ccctcaagcg tattaaccaa caattcaggg attaatggaa gatggcagac atcattgatt
     3001 cagcatcaga aattgaagaa ttacagcgca acacagcaat aaaaatgcgc cgcctgaacc
     3061 accaggctat atctgccact cattgttgtg agtgtggcga tcccatagat gagcgaagac
     3121 gcctggccgt tcagggttgt cggacttgtg caagttgcca ggaggatctg gaacttatca
     3181 gtaaacagag aggttcgaag tgagcgaaat taactagaag ccaaagataa aatcatcgct
     3241 gagcaggaga aaatcgctaa cggagaaaag acagtaagtc agtatatgaa aaccgcatga
     3301 tatcatcaga taaaaatcga tcgtaaagcg aaatattaat accagaataa acgagtcgag
     3361 gtaaattata ttacctcgat aaattaacta
//
LOCUS       DBFIFM010000308         3388 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.309, whole genome shotgun sequence.
ACCESSION   DBFIFM010000308 DBFIFM010000000
VERSION     DBFIFM010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.309"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..564
                     /gene="espA"
                     /locus_tag="N7C74_004600"
     CDS             <1..564
                     /gene="espA"
                     /locus_tag="N7C74_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HGU2434309.1"
                     /translation="ATSVVNVSASSSTSTIYDLGNMSKDEVVKLFEELGVFQAAILMF
                     SYMYQAQSNLSIAKFADMNEASKASTTAQKMANLVDAKIADVQSSTDKNAKAKLPQDV
                     IDYINDPRNDISVTGIRDLSGDLSAGDLQTVKAAISAKANNLTTVVNNSQLEIQQMSN
                     TLNLLTSARSDVQSLQYRTISAISLGK"
     gene            577..1701
                     /gene="espD"
                     /locus_tag="N7C74_004601"
     CDS             577..1701
                     /gene="espD"
                     /locus_tag="N7C74_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspD"
                     /protein_id="HGU2434310.1"
                     /translation="MLNVNNDTLSVTSGVNTASGTSGITQSETGLSLDLQLVKSMNSS
                     AGWTESSPLPTPPAGHSLVTPSAAEDVLSKLFGGISGEVTSRTEEAEPQRTSYPYLSQ
                     VNTVDPQQMMMMVTLLSLDTSAQKVSSLKNSNEIYMDGQTKALENKTQEYKKQLEEQQ
                     KAEEKSQKSKIVGQVFGWLGVALTAVAAVFNPALWAVVAIGATAMALQTAVDVMGENA
                     PQGLKTAAQVFGGISMAASILTAGVGGVSSLLSKFGNVANKIGSSVVKVVEKAAEALV
                     KNVFAKISTVAEGVTNGIRSAGTTALNNEAAQLQMLSQLAAFAVQNLTRQSESLGESA
                     KLELDKAASELQNQASYLQSVSQLMSDSARVNSRIVSGRI"
     gene            1722..2660
                     /gene="espB"
                     /locus_tag="N7C74_004602"
     CDS             1722..2660
                     /gene="espB"
                     /locus_tag="N7C74_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HGU2434311.1"
                     /translation="MNTIDNTQVTMVNSASESTTGASSAVAASALSIDSSLLTDGKVD
                     ICKLMLEIQKLLGKMVTLLQDYQQKQLAQSYQIQQAVFESQNKAIEEKKAAATAALVG
                     GIISSALGILGSFAAMNNAAKGAGEIAEKASSASSKAAGAASEVANKALVKATESVAD
                     VAEEASSAMQKAMATTTKAASRASGVADDVAKASDFAEDLADAAEKTSRINKLLNSVD
                     KLTNTTAFVAVTSLAEGTKTLPTTISESVKSTHEVNEQRAKSLENFQQGNLELYKQDV
                     RRTQDDITTRLRDITSAVRDLLEVQNRMGQSGRLAG"
     gene            2667..3074
                     /gene="cesD2"
                     /locus_tag="N7C74_004603"
     CDS             2667..3074
                     /gene="cesD2"
                     /locus_tag="N7C74_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HGU2434312.1"
                     /translation="MVDTFNDEVFNYYLEQKGYTIQKEFLCGSAFFIGWRIETPFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTHLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFVMNVNS"
     gene            3110..3331
                     /gene="escF"
                     /locus_tag="N7C74_004604"
     CDS             3110..3331
                     /gene="escF"
                     /locus_tag="N7C74_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle filament
                     protein EscF"
                     /protein_id="HGU2434313.1"
                     /translation="MNLSEITQQMGEVGKTLSDSVPELLNSTDLVNDPEKMLELQFAV
                     QQYSAYVNVESGMLKTIKDLVSTISNRSF"
BASE COUNT          987 a          647 c          835 g          919 t
ORIGIN      
        1 gcaacatccg ttgttaatgt gagtgcgagt tcttcgacat cgacgatcta tgacttaggt
       61 aatatgtcga aggatgaggt ggttaagcta tttgaggaac tcggtgtttt tcaggctgcg
      121 attctcatgt tttcttatat gtatcaggca caaagtaatc tgtcgattgc aaagtttgct
      181 gatatgaatg aggcatctaa agcgtcaacc acggcacaaa agatggctaa tcttgtggat
      241 gccaaaattg ctgatgttca gagtagcact gataagaatg cgaaagccaa acttcctcaa
      301 gacgtgattg actatataaa cgatccacgt aatgacataa gtgtaactgg tattcgtgat
      361 cttagtggtg atttaagcgc tggtgatctg caaacagtga aggcggctat ttcagctaaa
      421 gcgaataacc tgacaacggt agtgaataat agccagctcg aaattcagca aatgtcgaat
      481 acattaaatc tcttaacgag tgcacgttct gatgtgcaat ctctacaata tagaactatt
      541 tcagcaatat cccttggtaa ataaccggag ataactatgc ttaacgtaaa taacgatacc
      601 ctgtctgtaa cgtctggggt taataccgcc tcgggtactt ctggtattac tcaatctgaa
      661 acgggtttat cgctggattt acaactggtt aaatccatga actcgtcagc aggctggaca
      721 gaaagtagcc ctttaccgac gccgccggca ggtcactcat tagtgacgcc ctctgctgct
      781 gaggatgtcc ttagtaaatt gtttggtggt attagtggtg aggttacaag tcgcactgag
      841 gaggcagagc cacagcgcac aagctatccc tatctctctc aggtgaatac cgttgaccct
      901 cagcaaatga tgatgatggt cactctgtta tccctggata cttccgcgca gaaagtctcg
      961 agtctgaaaa actctaacga gatttatatg gatgggcaaa ctaaagcgct ggagaataaa
     1021 acgcaggagt ataaaaaaca gctcgaagaa caacagaaag ccgaagagaa atcacaaaaa
     1081 agtaaaattg ttggccaggt ctttggttgg ttgggcgtcg cattaacagc cgttgccgct
     1141 gtttttaacc cagcactctg ggctgttgtt gccattggtg caacagcaat ggcactgcaa
     1201 acggcagtcg atgtaatggg ggaaaatgcc cctcagggat taaagactgc agcacaggtc
     1261 tttggcggaa tatctatggc cgcaagcatt ctgacagccg gcgttggcgg ggtgtcttca
     1321 ctgttatcta aatttggtaa tgttgctaac aaaattggct caagcgttgt aaaagtcgtt
     1381 gagaaggcgg cagaagcgct ggttaaaaac gtttttgcaa aaatttcgac agtggctgag
     1441 ggcgttacga acggtattcg ttctgccggg acaactgcgt tgaataatga ggctgcgcaa
     1501 ctccaaatgt tgtctcagtt agctgctttc gcggtgcaaa acttaactcg acagagtgaa
     1561 agcttaggtg agagtgcgaa gctcgagctg gataaagcgg caagcgagtt acaaaatcag
     1621 gcgagctatt tacaaagtgt ttctcaactg atgtccgatt cagcacgggt aaatagtcgt
     1681 attgttagtg gccgaattta attattaaag agaattttat tatgaatact attgataata
     1741 ctcaagtaac gatggttaat tccgcttcgg agagtacgac cggcgcttcc agtgcagttg
     1801 ccgcatctgc tttatcaatt gattcatctc tgcttactga tggtaaggtt gatatttgta
     1861 agctgatgct ggaaattcaa aaactcctcg gcaagatggt gactctattg caggattacc
     1921 aacaaaaaca attggcgcaa agctatcaga ttcagcaggc cgtttttgag agccagaata
     1981 aagctattga ggaaaaaaaa gccgcggcaa ccgctgcttt ggttggcggg attatttcat
     2041 cagcattggg gatcttaggt tcttttgcag caatgaacaa cgcggctaaa ggggctggtg
     2101 agattgctga aaaagcaagc tctgcatctt caaaggctgc tggtgcggct tctgaggttg
     2161 caaataaagc tctggtcaag gctacggaaa gtgttgctga tgtcgcagag gaggcatcca
     2221 gtgcgatgca gaaagcgatg gccacaacaa cgaaagcagc cagccgtgca tctggcgttg
     2281 cagatgatgt tgcgaaagcc tctgactttg ctgaagatct tgcagacgcc gccgagaaga
     2341 caagcagaat caataagttg ttgaattccg tagataaact gaccaatacc acagcatttg
     2401 ttgccgtgac cagtcttgct gaaggtacga aaacgttgcc aacaacaata tctgagtccg
     2461 tcaaatcgac tcatgaggtt aatgaacaac gtgcgaagtc gctggaaaac ttccagcagg
     2521 ggaatctgga gctgtataaa caagacgttc gcagaacgca ggatgatatc acgactcgtc
     2581 tgcgtgatat aacgtccgct gtccgcgatc tccttgaggt ccagaatcgt atggggcaat
     2641 cgggtcgctt agctgggtaa ttgatcatgg tcgatacgtt taatgatgaa gtgtttaatt
     2701 actatcttga acaaaaaggg tacacaatac agaaggagtt tctttgtggc agtgcctttt
     2761 ttatcggatg gcggattgag acaccttttt tttcattagc gtacagactg gatgaacaag
     2821 aactgatttt gtgctctttt gaagcacgta accaaacagg gcttaacggc cctgttttat
     2881 cgctgactca tttgctcgaa gagttgtatc accatttttc gggtattaag aaaatcagcg
     2941 cgatgaaatc taagattggt tcagattcag aacgtcaaaa gcgcgaagag ttgtttaatt
     3001 acttcatcag aaagggtgcc gtgcagcaag aaacagaaga cggaatttgg ttcgtaatga
     3061 acgtaaatag ttaaaagttt ttattttatt aacttctgag ggaaatttaa tgaatttatc
     3121 tgaaattact caacaaatgg gtgaagtagg taaaacgctg agcgattctg tgccagagtt
     3181 acttaatagc accgatttgg ttaatgaccc tgaaaaaatg ttagagttgc agtttgcggt
     3241 tcagcaatat tctgcttatg ttaacgtaga aagtggaatg ttgaaaacga taaaagatct
     3301 ggtctcaacc atttctaacc gtagttttta attttaatgg ttaatgatat ttctgctaat
     3361 aagatactgg tgtgggcggc tgtagctg
//
LOCUS       DBFIFM010000309         3375 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.310, whole genome shotgun sequence.
ACCESSION   DBFIFM010000309 DBFIFM010000000
VERSION     DBFIFM010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.310"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..152
                     /locus_tag="N7C74_004605"
     CDS             <1..152
                     /locus_tag="N7C74_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WlaTC/HtrL family glycosyltransferase"
                     /protein_id="HGU2434314.1"
                     /translation="IFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALRI
                     FLSRK"
     gene            427..1623
                     /gene="kbl"
                     /locus_tag="N7C74_004606"
     CDS             427..1623
                     /gene="kbl"
                     /locus_tag="N7C74_004606"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HGU2434315.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            1633..2658
                     /gene="tdh"
                     /locus_tag="N7C74_004607"
     CDS             1633..2658
                     /gene="tdh"
                     /locus_tag="N7C74_004607"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HGU2434316.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMCSGQSG
                     KVILSWD"
     gene            2897..>3375
                     /gene="waaH"
                     /locus_tag="N7C74_004608"
     CDS             2897..>3375
                     /gene="waaH"
                     /locus_tag="N7C74_004608"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HGU2434317.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWSFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     "
BASE COUNT          797 a          774 c          914 g          890 t
ORIGIN      
        1 gcatttttgt catgtgctat tacaaacgcc ccgatctctt caacctaaac tatctgggga
       61 gaggaaaatg gttcgatttg tttcgctgct tcaggagtaa cactttaggg gcaaaaatgc
      121 aggcgctgag aattttcctt tcaagaaaat aggtttccct ccattagtgg gtagcgtttt
      181 gataccaact gagataggaa aaatgatgct ttggcatcat tttttctttt tatatcaatg
      241 cagtaaaatc aatcatcatt atgcccagcc aaaactgtac agtttttcta ctatctgtag
      301 gtgttgagag acatcaagtt acacgtcatt tatcctgaat tttgcagaag agttaacgcg
      361 tcatctcgtc gcgacctata agtttgggta atatgtgctg gaatttgccc tgtctggaga
      421 atcgcaatgc gtggagaatt ttatcagcag ttaaccaacg atctggaaac cgcacgggcg
      481 gaagggttgt ttaaagaaga gcgcattatt acgtctgcgc agcaagcaga tatcactgtg
      541 gctgatggaa gccacgtcat taacttttgt gccaacaact atctcgggct ggcgaatcat
      601 ccggatctga ttgcggcagc aaaggcggga atggattctc acggtttcgg catggcttcg
      661 gtgcgtttta tttgcggcac tcaggacagc cataaagagc ttgaacaaaa actggcggca
      721 ttcctgggga tggaagatgc gattctctac tcttcctgct ttgatgctaa cggtggactg
      781 tttgaaacgc tgctgggtgc ggaagacgcc attatctccg acgcactgaa ccacgcgtct
      841 attattgatg gtgtgcgtct gtgcaaagct aaacgctatc gctatgccaa caacgatatg
      901 caggagctgg aagcacgtct gaaagaagcg cgtgaagccg gtgcgcgtca tgtgctgatt
      961 gccaccgatg gtgtgttctc aatggacggc gtgattgcca acctgaaagg cgtttgcgat
     1021 ctggcagata aatacgatgc cctggtgatg gtggatgact cccacgcggt cggttttgtc
     1081 ggtgaaaatg gtcgtggttc ccatgaatac tgcgatgtga tgggccgggt cgatattatc
     1141 accggtacgc ttggtaaagc gctgggcggg gcttctggtg gttataccgc ggcgcgcaaa
     1201 gaggtggttg agtggctgcg ccagcgttct cgtccgtacc tgttctccaa ctcgctggca
     1261 ccggccattg ttgccgcgtc catcaaagta ctggagatgg tcgaagcggg cagcgaactg
     1321 cgtgaccgtc tgtgggcgaa cgcgcgccag ttccgtgagc aaatgtcggc ggcgggcttt
     1381 accctggcgg gagccgatca tgccattatt ccggtcatgc ttggcgatgc agtagtggcg
     1441 cagaaatttg cccgtgagct gcaaaaagag ggcatttacg tcaccggttt cttctatccg
     1501 gtcgttccga aaggtcaggc gcgtattcgt acccagatgt ctgcggcgca tacccctgag
     1561 caaattacgc gtgcagtaga agcattcacg cgtattggta aacaactggg cgttatcgcc
     1621 tgaggatgtg agatgaaagc gttatccaaa ctgaaagcgg aagagggcat ctggatgacc
     1681 gacgttcctg taccggaact cgggcataac gatctgctga ttaaaatccg taaaacagcc
     1741 atctgcggga ctgacgttca catctataac tgggatgagt ggtcgcaaaa aaccatcccg
     1801 gtgccgatgg tcgtaggcca tgaatatgtc ggtgaagtgg taggtattgg tcaggaagtg
     1861 aaaggcttca aaatcggcga tcgcgtttct ggcgaaggcc atatcacctg cggtcactgt
     1921 cgcaactgtc gtggcggtcg tacccatctg tgccgcaaca ccattggtgt cggtgttaac
     1981 cgtccgggct gcttcgccga atatctggtg attccggcgt tcaacgcttt caaaatcccc
     2041 gacaatatct ccgacgacct ggcttccatt tttgatccct tcggtaacgc cgtgcatacc
     2101 gcgctgtcgt tcgatctggt gggcgaagat gtgctggttt ctggtgcagg tccgataggt
     2161 attatggctg cggcggtggc gaaacacgtt ggtgcacgca atgtggtgat caccgatgtt
     2221 aacgaatacc gccttgagct ggcgcgcaaa atgggtatca cccgtgcggt taacgtcgcg
     2281 aaagaaaacc ttaatgatgt gatggctgaa ctgggcatga ccgaaggctt tgatgtcggt
     2341 ctggaaatgt ccggcgcgcc gccagcgttt cgtaccatgc tcgataccat gaaccacggc
     2401 ggtcgtattg cgatgctggg tattccaccg tctgacatgt ctatcgactg gaccaaagtg
     2461 atctttaaag gcttgttcat taaaggcatt tacggtcgtg agatgtttga aacctggtac
     2521 aagatggcgg cgctgattca gtctggcctc gatctctcgc cgatcattac ccatcgtttc
     2581 tctatcgatg atttccagaa gggctttgac gctatgtgtt cgggccagtc cgggaaagtt
     2641 attctgagct gggattaaca cgaaaaaggg ctggtattcc agcccttttg cctgaggata
     2701 atctgttaaa tatgtaaaat cctgtcagtg taataaagag ttcgtaattg cgctgatctt
     2761 ttatatagct gttctcatta tctctctacc ctgaagtgac tctctcacct gtaaaaataa
     2821 tatctcacag gcttaatagt ttcttaatac aaagcctgta aaacgtcagg ataacttcag
     2881 aggtcgtcgg taatttatga tgaacagcac caataaactt agtgttatta ttccgttata
     2941 taatgcgggc gatgatttcc gcacttgtat ggaatcttta attacgcaaa cctggactgc
     3001 tctggaaatc attattatta acgatggttc aacggataat tctgttgaaa tagcaaagca
     3061 ttacgcagaa aactatccgc acgttcgttt gttgcatcag gcgaatgctg gcgcatcggt
     3121 agcgcgtaat cgtgggatcg aggtggcgac gggcaaatat gtcgcttttg tcgatgctga
     3181 cgatgaggtc tatcccacca tgtacgaaac gctgatgacc atggcgttag aggacgacct
     3241 cgacgtggcg cagtgcaacg ctgactggtc ttttcgtgaa acgggagaaa cctggcaatc
     3301 catccccagc gatcgccttc gctcaaccgg tgtattaacc ggcccggact ggctgcggat
     3361 ggggctttct tcgcg
//
LOCUS       DBFIFM010000310         3348 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.311, whole genome shotgun sequence.
ACCESSION   DBFIFM010000310 DBFIFM010000000
VERSION     DBFIFM010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.311"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..703
                     /locus_tag="N7C74_004609"
     CDS             <1..703
                     /locus_tag="N7C74_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HGU2434318.1"
                     /translation="VWFLNVITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIAF
                     DDAVRRTLKEEEKLVNSSDWGYDTQAFARWRPEYGYFAKQAGFTVKTSASLAALWQVV
                     NQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEK
                     QLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFR
                     GMAKRIARLAEQSTD"
     gene            802..1815
                     /gene="ybjS"
                     /locus_tag="N7C74_004610"
     CDS             802..1815
                     /gene="ybjS"
                     /locus_tag="N7C74_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HGU2434319.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(1812..2642)
                     /gene="amiD"
                     /locus_tag="N7C74_004611"
     CDS             complement(1812..2642)
                     /gene="amiD"
                     /locus_tag="N7C74_004611"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HGU2434320.1"
                     /translation="MRRVFWLIAVALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(2639..2962)
                     /gene="ybjQ"
                     /locus_tag="N7C74_004612"
     CDS             complement(2639..2962)
                     /gene="ybjQ"
                     /locus_tag="N7C74_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HGU2434321.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(3173..>3348)
                     /locus_tag="N7C74_004613"
     CDS             complement(3173..>3348)
                     /locus_tag="N7C74_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434322.1"
                     /translation="EFARMIFAYINDKSIEILLLSCFAAADQKAIQRYIPQAQDGEDF
                     RNHLQYKLTLPTH"
BASE COUNT          778 a          907 c          856 g          807 t
ORIGIN      
        1 ggtgtggttt ctcaatgtga ttacttccgt accgcccacc accgccaggg cgttgattca
       61 ggggctgaaa cacgatctgc tggcggatga caccgcgcta cgtgcgctca tcccacaacg
      121 gctgatcgct ttcgatgacg cggtacgtcg caccctgaaa gaagaagaaa agctggtcaa
      181 ctccagcgac tggggatacg acactcaggc ctttgcccgc tggcgaccag agtatggtta
      241 ttttgccaaa caggcgggat ttaccgttaa aacgtccgcc agccttgcgg ctttatggca
      301 ggtggtgaac caaatcggcg gtaaagagcg ttatttcttt ggcaatattt tgtggcagac
      361 acgggcgttg atggaccgtg cgatcggtca taaattagcg aaaggccgtc cggagcgcga
      421 atatttgcaa actggcgatg cggtggatag ctggaaagtg attgtcgttg aaccggaaaa
      481 acaacttacg ttgttatttg gcatgaaagc accggggctg ggacgactgt gttttaccct
      541 ggaagataaa ggcgactatc gtactatcga tgtccgcgct ttctggcatc cgcacggtat
      601 gccggggctg ttttactggt tattgatgat ccccgcgcat ctgtttattt ttcgcggaat
      661 ggcaaaacga atcgccagac tggcagaaca aagcacagat taatcgacaa aacctgcgtt
      721 attctttcat gtttcccatt gcatcaaggg ggaaatcacg gaagaatgcg cacgtcattt
      781 tctcaacaac agtggattga tatgaaggta ctggttaccg gcgccaccag cggcttaggt
      841 cgaaacgcgg tagagttttt atgccagaaa ggcatcagcg tgcgagcgac cggtcgcaac
      901 gaggcaatgg gtaaattgct ggaaaaaatg ggcgcagagt ttgttccggc ggatctgacc
      961 gagctggtct catcacaagc taaagtgatg ctcgcgggca ttgatacgct gtggcactgc
     1021 tccagcttta cctctccctg ggggacacaa caggctttcg atctggctaa cgttcgcgcc
     1081 actcgccgcc taggtgaatg ggctgtcgcc tggggtgtac gtaactttat tcatatctct
     1141 tctccctccc tgtacttcga ttatcaccac catcgcgata ttaaagaaga ttttcgccct
     1201 caccgcttcg ccaacgagtt tgcccgcagc aaagcggcca gcgaagaagt gatcaatatg
     1261 ctttcgcagg cgaatccaca aacgcgcttt actattctgc gcccacaaag tctgttcgga
     1321 ccgcacgata aagtctttat cccccgtctt gcgcatatga tgcaccacta cggcagcatt
     1381 ctgttaccgc atggcggcag tgcgctggtg gatatgacct actatgaaaa tgccgtgcac
     1441 gccatgtggc tggcaagcca ggaagcctgc gataagctac cttccgggcg tgtgtacaac
     1501 atcaccaacg gcgagcatcg cacactgcgc agcatcgtgc agaagctgat cgacgagttg
     1561 aatattgact gtcgtattcg ttccgtccct tacccgatgc tggatatgat cgcccgcagc
     1621 atggagcgtt taggccgcaa gtcagcaaaa gagccgccgc tgacccacta cggcgtctcg
     1681 aagcttaatt ttgactttac gctggatatt acgcgggcac aggaagagtt aggttatcag
     1741 ccggtcatca ccctggatga aggtatcgag aaaactgccg cctggctgcg cgaccacgga
     1801 aaactgccgc gctaatcctg cccgtatttc tccagcaacg cttcggcaat cgcctgagtt
     1861 tctgcatccg cttcgccgtt atataacgtc gggcggaaat gcatctggaa cgccataatc
     1921 acgcgccgct gctcgcgcgg tgtcatatca ggtttaacgt cataaccgta gcgcgccaaa
     1981 agctccagca atgacgcggt atctaccggt gtgtgcggcg cgcgcccggc aaggtaaaag
     2041 ttcacccgct gcgcatccgg ccaggcacca atcccctgct gcgccagttg ctgccaggga
     2101 aataatggcc ccggatcgtc tttgcgctgc ggggcgatat ccgcatgtgc cactacgttt
     2161 tccggcttga tgtgataacg ggcaataata tctttcgcca gcggaataag cgcctgaatc
     2221 tgtgccggtt caaacggggc aaaatatttc acccccgccg atttttgcca gccacggttt
     2281 tccagctcaa tgccaataga ggtgtcgtta aggcgcgttg ccccgcgcca ggcgctaatc
     2341 cccgcatgcc aggccagttc ttgttccggc accaattgcc agatgcgcgg tttaccgttg
     2401 tatcgcggtg gtaccgcagg gaccagataa tgcgagctga cctgcttatc ggtcagtgtc
     2461 gccagcgagc tatcaaaatc atctgcggtg tagtggatca ccagcacttt aatgcgcgga
     2521 tacgccgcct gcgcctggcg tcgggtatca agctgatatc cctctttctc gacaatgcct
     2581 ttttcgcctg cgcaccctgc cagtaacaga gcgacagcga tcagccagaa gactcttctc
     2641 atcgacgcgt tttcaccgcc gtaccgctga cgctaaccat cagcatactg ccgttttgcc
     2701 cgaccgtttc gtagtcgata tcaataccga ctacggcatc ggcccccagc gcccgcgcct
     2761 gggagccaag ctcctcaaag gcgatctccc gcgctttacg tagctctttt tcgtaggcac
     2821 cggagcgtcc gccaacgata tcgcgaatac cagcaaagaa atcacggaaa atattcgcgc
     2881 ctaaaatcgc ttcgccagtc accacaccgc aatattcaac gatggtctgg ccttccagag
     2941 ttggagttgt ggaaaattgc atgatcgatc tccttgtttg ttaagccttc gatctctaaa
     3001 gcattatcgg actgatatgc catgttggga agaaggtttt gatcagttta ggaaatcatc
     3061 tcattgtgtc aaaaacagct acataagcct gtacaattat ctgtttcgta accggatatt
     3121 ggtttcatta tggagcaccg cttacctgcc ctattatgtt gtgccggcaa cattaatgag
     3181 tgggaagagt caacttatat tgtaaatgat tcctaaagtc ttccccatct tgcgcctgag
     3241 gtatataccg ctggattgct ttttggtccg ccgccgcaaa acaagaaagt aataagatct
     3301 caatgctttt gtcattaata taagcgaaga tcattcttgc aaattcgt
//
LOCUS       DBFIFM010000311         3345 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.312, whole genome shotgun sequence.
ACCESSION   DBFIFM010000311 DBFIFM010000000
VERSION     DBFIFM010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.312"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            211..417
                     /gene="ybcW"
                     /locus_tag="N7C74_004614"
     CDS             211..417
                     /gene="ybcW"
                     /locus_tag="N7C74_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbcW"
                     /protein_id="HGU2434323.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLE
                     AFRAIEAALVKHDNNMKDYSLVVD"
     gene            complement(582..776)
                     /locus_tag="N7C74_004615"
     CDS             complement(582..776)
                     /locus_tag="N7C74_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU2434324.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALDQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIKSDEE"
     gene            1165..1710
                     /locus_tag="N7C74_004616"
     CDS             1165..1710
                     /locus_tag="N7C74_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HGU2434325.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AAIKWYAERDAEIENEKLRREVEELRQDSETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1685..>3345
                     /locus_tag="N7C74_004617"
     CDS             1685..>3345
                     /locus_tag="N7C74_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGU2434326.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSASVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDL"
BASE COUNT          838 a          749 c          910 g          848 t
ORIGIN      
        1 ctttgtgttt tgttgatgat ttatgtcaaa tattaggaat gttttcaatt aatagtattg
       61 gttgcgtaac aaagtgcggt cctgctggca ttctggaggg aaatacaacc gacagatgta
      121 tgtaaggcca acgtgctcaa atcttcatac agaaagattt gaagtaatat tttaaccgct
      181 agatgaagag caagcgcatg gagcgacaaa atgaataaag aacaatctgc tgatgatccc
      241 tccgtggatc tgattcgtgt aaaaaatatg cttaatagca ccatttctat gagttaccct
      301 gatgttgtaa ttgcatgtat agaacataag gtgtctctgg aagcattcag ggcaattgag
      361 gcagcgttgg tgaagcacga taataatatg aaggattatt ccctggtggt tgactgatca
      421 ccataactgc taatcattca aactacttaa cctgtgacag agccaacacg cagtctgtca
      481 ctgtcaggaa agtggtaaaa ctgcaactca attactgcaa tgccctcgta attaagtgaa
      541 tttacaatat cgtcctgttc ggagggaaga acgcgggatg ttcattcttc atcactttta
      601 attgatgtat atgctctctt ttctgacgtt agcctccgac ggcaggcttc aatgacccag
      661 gctgagaaat ttccggaccc tttttgatca agagcgatgt taatttgttc aatcatttgg
      721 ttaggaaagc ggatgttgcg ggttgttgtt ctgcgggttc tgttcttcgt tgacatgagg
      781 ttgccccgta ttcagtgtcg ctgatttgta ttgtctgaag ttgtttttac gttaagttga
      841 tgcggatcaa ttaatacgat acctgcgtca taattgatta tttgacgtgg tttgatggcg
      901 tagatgcacg ttgtgacatg tagatgataa ttattatcat tttgcgggtc ctttccggcg
      961 atccgacagg ttacggggcg gcgacctcgc ggtttttcac tatttatgaa aattttccgg
     1021 tttaaggcgt ttccgttctt cttcgccgta acctaatgtt tttatttaaa acaccccctg
     1081 aaaagaaagg aaacgacagg tgctgaaaac gggctttttg gcctctgtcg tttcctttct
     1141 ctgtttttgt ccgtggaatg aacaatggaa gtcaacaaaa agcagctggc tgacattttc
     1201 ggcgcgagta tccgtaccat tcagaactgg caggagcagg gaatgcccgt tctgcggggt
     1261 ggtggcaagg gtaatgaggt actttatgac tctgccgccg ccataaaatg gtatgccgaa
     1321 agggatgctg aaattgagaa cgaaaagctg cgccgggaag ttgaagaact gaggcaggac
     1381 agcgagacag atctccagcc agggactatt gagtacgaac gccatcgact tacgcgtgcg
     1441 caggccgacg cacaggaact gaagaatgcc agagactcag ctgaagtggt ggaaaccgca
     1501 ttctgtactt tcgtgctgtc gcggatcgca ggtgaaattg ccagtattct cgacgggatc
     1561 cccctgtcgg tgcagcggcg ttttccggaa ctggaaaacc gacatgttga tttcctgaaa
     1621 cgggatatca tcaaagccat gaacaaagca gccgcgctgg atgaactgat accggggttg
     1681 ctgagtgaat atatcgaaca gtcaggttaa caggctgcgg cattttgtcc gcgccgggct
     1741 tcgctcactg ttcaggccgg agccacagac cgccgttgaa tgggcggatg ccagttacta
     1801 tctcccgaaa gaatccgcat accaggaagg gcgctgggaa acactgccct ttcagcgggc
     1861 catcatgaat gcgatgggca gtgactacat ccgcgaggtg aatgtggtga agtctgcccg
     1921 tgttggttat tccaaaatgc tgctgggtgt ttatgcctac ttcatagagc ataagcagcg
     1981 caacaccctt atctggttgc cgacggatgg tgatgccgag aactttatga aaacccacgt
     2041 tgagccgact attcgtgata ttccgtcgct gctggcgctg gccccgtggt atggcaaaaa
     2101 gcaccgggat aacacgctca ccatgaagcg tttcaccaat gggcgtggct tctggtgcct
     2161 gggcggtaaa gcggcaaaaa actaccgtga aaagtcagtg gatgtggcgg gttatgatga
     2221 acttgctgcc tttgatgagg atattgaaca ggaaggctct ccgacgttcc tgggcgataa
     2281 gcgtattgaa ggctcggtct ggccaaagtc catccgtggc tccacgccca aagtgagagg
     2341 cacctgccag attgagcgtg cagccagtga atccccgcat tttatgcgtt ttcatgttgc
     2401 ctgcccgcac tgcggggagg agcagtacct taaatttggc gataaagaga cgccgtttgg
     2461 cctcaaatgg acgccggatg atccctccag cgtgttttat ctctgcgagc ataatgcctg
     2521 cgtcatccgt cagcaggagc tggactttac tgatgcccgt tatatctgcg aaaagaccgg
     2581 gatctggacc cgtgatggca ttctctggtt ttcgtcatcc ggtgaagaga ttgaaccgcc
     2641 tgacagtgtg acctttcaca tctggacggc gtacagcccg ttcaccacct gggttcagat
     2701 tgtcaaagac tggatgaaga cgaaagggga tacgggaaaa cgtaaaacct tcgtgaacac
     2761 cacgctcggt gagacatggg aagcgaagat cggcgaacgt ccggatgctg aagtgatggc
     2821 agagcggaaa gagcattatt cagcgtccgt tcctgaccgt gtggcttacc tgaccgccgg
     2881 tatcgactcc cagctggatc gctacgaaat gcgcgtatgg ggatgggggc cgggtgagga
     2941 aagctggctg attgatcggc agattattat gggccgccac gacgatgaac agacgctgct
     3001 gcgtgtggat gaggccatca ataaaaccta tacccgccgg aatggtgcag aaatgtcggt
     3061 atcccgtatc tgctgggata ctggcgggat tgacccgact attgtgtatg aacgctcgaa
     3121 aaagcatggg ctgttccggg tgatccccat taaaggggca tccgtctacg gaaagccggt
     3181 ggccagcatg ccacgtaagc gaaacaaaaa cggggtttac cttaccgaaa tcggtacgga
     3241 taccgcgaaa gagcagattt ataaccgctt cacactgacg ccggaagggg atgaaccgct
     3301 tcccggtgcc gttcacttcc cgaataaccc ggatattttt gatct
//
LOCUS       DBFIFM010000312         3326 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.313, whole genome shotgun sequence.
ACCESSION   DBFIFM010000312 DBFIFM010000000
VERSION     DBFIFM010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.313"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..384
                     /locus_tag="N7C74_004618"
     CDS             <1..384
                     /locus_tag="N7C74_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU2434327.1"
                     /translation="LAEFVALISESGANPFGLTVDAVMEEYRRWRNESWRYDGSDKYP
                     WPQPVLYHICLEMRDRGIERQMTEGELKRLAERQLTKWAKQVGNGMSIPPIRRQLASP
                     KCPQGPTPIELLKQEYERRKAAGFV"
     gene            418..1134
                     /locus_tag="N7C74_004619"
     CDS             418..1134
                     /locus_tag="N7C74_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU2434328.1"
                     /translation="METVSDALKALKRASSHVVAARLGISREEAVNELWKLKRRGEAD
                     NKGAIWWLTQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGCLIRVNQDGKFRYCIPGVDLPAEPKAASVAETEGKALPQPAGVALPVQETA
                     AQEEIKTEAVEDIVKLQQSFTEAKADDLILPSLHVANRELRRAKSNVQKWERVCAALR
                     ELNKHRDILRDITATREQQR"
     gene            1131..1448
                     /locus_tag="N7C74_004620"
     CDS             1131..1448
                     /locus_tag="N7C74_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU2434329.1"
                     /translation="MSGWKKWRWAEILILRQCAGTMRVESIGYLIGRSESAVRTKARE
                     LGISMMLRGDYHQSAKCSQRDIELAWQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRR
                     VQE"
     gene            1445..1747
                     /locus_tag="N7C74_004621"
     CDS             1445..1747
                     /locus_tag="N7C74_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HGU2434330.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKTEEVLKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMLRCVDAVYMLKGWQQSAGAGAELALEEKPGHAVIFQEVGSEY"
     gene            1737..2054
                     /locus_tag="N7C74_004622"
     CDS             1737..2054
                     /locus_tag="N7C74_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HGU2434331.1"
                     /translation="MNIDPAITIDMALNAGLALLGYFYIMFCSGRWLSLLFMKKWNKR
                     RKQEQRQKAMDAFFEAFGIDGMEPGDPARAISRGGVVILVYRSEEKNDDHKTTCRRNH
                     IPH"
     gene            2008..2325
                     /locus_tag="N7C74_004623"
     CDS             2008..2325
                     /locus_tag="N7C74_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434332.1"
                     /translation="MTITKQRVEEIISRIEMYGHGAGYIADEVNDLAILALNLSNIAN
                     LKRYELDMGGCDSCGQDCGADMTEDPDGDYVLFDDVVKLFEFDTTTQKLEIPAKEAAS
                     GQD"
     gene            2312..2749
                     /locus_tag="N7C74_004624"
     CDS             2312..2749
                     /locus_tag="N7C74_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU2434333.1"
                     /translation="MGKIDYQALREAAQNYQSTLAWYQATPDSPNAERDCDAALAAFK
                     RHIRHREADIIADLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELEAREVQLPTRYD
                     LRYGHPINADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGE"
     gene            2751..2942
                     /locus_tag="N7C74_004625"
     CDS             2751..2942
                     /locus_tag="N7C74_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434334.1"
                     /translation="MNGQISIVRPGACDDREIRMIIRLAMGKTITALITPENLALALT
                     GKSDMPVELKLRNVEIKVK"
     gene            2945..>3326
                     /locus_tag="N7C74_004626"
     CDS             2945..>3326
                     /locus_tag="N7C74_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434335.1"
                     /translation="MTTITKDRLLTIQHWRETYGPGSNVVLPAEEAEELARIALASLA
                     AVSDERAAYELFMEKRFGESVDRRRAKNGDRDYMVWDMALGWIIWCHRAAMLQAGNFR
                     ENKGSSTNNFREISETSTNYPVTPD"
BASE COUNT          920 a          693 c          992 g          721 t
ORIGIN      
        1 ctggctgagt ttgtggcgtt gatttcggag agcggggcaa atccatttgg cctgacggtg
       61 gatgccgtga tggaggagta ccgtcgctgg aggaatgaat cctggcggta cgacgggagt
      121 gataaatacc cgtggccaca gccggtgctg taccacattt gcctcgagat gcgtgacaga
      181 gggattgagc ggcagatgac ggaaggtgag ttaaaacgac ttgcagaacg gcaactgacg
      241 aaatgggcaa agcaagttgg taacgggatg agtattcccc cgatccggag acaactcgca
      301 tcgccgaagt gcccgcaagg gccaacgcca attgaattac tgaaacagga atacgagcgc
      361 cggaaggcgg ctggttttgt ttgaatctga gaaacgattt tgtcggagga aattttaatg
      421 gaaaccgtat ctgacgcact gaaagcactg aaaagagcct cttcacatgt ggtggcagct
      481 cgccttggaa ttagtcgtga agaggctgtc aacgaactgt ggaaactgaa acgccgtgga
      541 gaagcggata acaagggggc gatatggtgg ctgactcagg ctggtgaaag tgaaccggtg
      601 tcaccggtac cgaaagtgac ggcgcaaatg ctgacagagg cgattgaaca acatggccca
      661 caaacggcgg atgaactggc attgatgttc ggaattacct cccgccgggc gaattcatcg
      721 ctggcaatgg caatcagcaa agggtgtctg attcgcgtga atcaggatgg taaatttcgt
      781 tactgcatac cgggcgttga tttaccggca gagccgaaag cagcatccgt agctgaaacg
      841 gagggtaaag cccttcctca gccagctggt gttgcgttac cagtccagga aacggctgca
      901 caggaagaaa ttaaaacaga agcggtggag gacattgtga agttgcagca atcgttcact
      961 gaagcgaaag cagatgacct gattctacca tcgctgcatg tggctaaccg cgagctgcgc
     1021 cgggcgaaaa gtaatgttca gaagtgggag cgagtctgtg ctgcgctacg ggaactgaac
     1081 aaacacaggg atattctccg ggatattacc gccaccagag agcagcagcg gtgagtggct
     1141 ggaagaagtg gcgctgggct gaaatcctga tactccggca gtgtgcggga acgatgagag
     1201 tcgaaagcat cggttatctg attggccgta gtgagtcagc cgtcaggacg aaagcgcggg
     1261 aactgggtat cagcatgatg ttacggggtg attatcacca gtcagccaaa tgttcacagc
     1321 gtgatattga gctggcgtgg caactgcatc agcgtggtgt accccgacgg gaaattgccg
     1381 aaaagtttgg gatgaagttg ggcgcagtga ataactacgt ttatttcgac aggagggttc
     1441 aggagtgagg gtgagggttt atatcgccgg tccgatgacg ggatatgaaa attttaaccg
     1501 cgaggcattt cacaaaacgg aagaggtgct gaaacgggaa gggcataccg ttttaaaccc
     1561 ggcagtactt ccggacgggc tgactcagcc acactacatg gatatttgca tggcaatgct
     1621 ccgttgcgtg gatgcggttt acatgctgaa aggctggcag cagtcggcag gtgcaggggc
     1681 tgagctggca ctggaggaga aaccgggcca tgcggtgatt tttcaggagg tgggcagtga
     1741 atattgaccc ggcgataacg attgatatgg ccctgaacgc cggcctggca cttcttggtt
     1801 atttctacat tatgttctgc agcggacgat ggctgtcact gttgttcatg aaaaaatgga
     1861 ataaacgccg taagcaggag caacgccaga aggcaatgga tgcatttttc gaagccttcg
     1921 gaattgacgg catggaacca ggggatccag ctcgcgcaat cagcagaggg ggtgtagtaa
     1981 tccttgtata tcggagtgaa gagaaaaatg acgatcacaa aacaacgtgt agaagaaatc
     2041 atatcccgca ttgaaatgta tggacatggt gcagggtata tcgcagacga ggttaatgat
     2101 ctggctatac tggcgctgaa tttatcaaat atcgcgaacc tgaagcgata cgagcttgat
     2161 atgggaggtt gcgactcgtg cggtcaggat tgtggcgctg atatgactga agatcctgat
     2221 ggtgattatg tcctgtttga tgacgtggtt aagttgtttg aatttgatac aaccactcaa
     2281 aagttagaaa tcccggcaaa ggaggcagcc agtgggcaag attgactatc aggcactgcg
     2341 tgaggcagca caaaactatc aatcgacgct ggcgtggtat caggctaccc cggacagccc
     2401 aaatgctgaa cgggattgtg atgcggctct tgctgcgttt aagcgtcaca tccgtcatcg
     2461 ggaagcggat attatcgctg atttgctgga tggactggaa gaagcaaaat cacaactcaa
     2521 cgagcagcgt gagtattacg aaggcgttat ctctgatggg agcaagcgta ttgctgaact
     2581 ggaagcgcgg gaagttcaat taccgactcg ctacgacctt cgatatggac acccgataaa
     2641 tgcagatgag cgacatgtca tgatacctaa agaaaatggc agttggcttt acctgattga
     2701 cctagaacac gcattacgcg tcgctggcat tcgcatcaaa ggagagtgag atgaacggac
     2761 aaatatcaat tgttcgacca ggagcatgtg acgatcgcga aatacgaatg attattcgtc
     2821 tggcgatggg gaaaacaata actgctctca ttactccaga aaatctcgca ttagcattaa
     2881 caggaaagtc agacatgcca gtagagctaa agctgcgaaa tgttgagatt aaggtgaaat
     2941 agctatgacc actattacca aagatcgact gctgacaatc cagcattggc gcgaaacata
     3001 cggaccgggt agcaacgttg tgcttccagc agaagaagcg gaagagctgg cacggattgc
     3061 gctggcatca ctggcagcag tatcggatga acgagcagcc tatgaattat ttatggagaa
     3121 gcgtttcgga gaatctgtag atcgccgcag agcaaaaaat ggcgatagag attacatggt
     3181 atgggatatg gcgcttggct ggattatctg gtgtcaccgc gccgccatgc ttcaggctgg
     3241 aaactttcgg gaaaataagg gttcgtcaac caataatttt cgggaaatct cggaaacgtc
     3301 aaccaactat ccggtaactc cggatg
//
LOCUS       DBFIFM010000313         3300 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.314, whole genome shotgun sequence.
ACCESSION   DBFIFM010000313 DBFIFM010000000
VERSION     DBFIFM010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.314"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..135)
                     /gene="escO"
                     /locus_tag="N7C74_004627"
     CDS             complement(<1..135)
                     /gene="escO"
                     /locus_tag="N7C74_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase activity
                     positive regulator EscO"
                     /protein_id="HGU2434336.1"
                     /translation="MAKINSQIKEVDGKLDDCEQSIKESIASKQAYCASLVNLDKVSL
                     Y"
     gene            complement(195..1535)
                     /gene="escN"
                     /locus_tag="N7C74_004628"
     CDS             complement(195..1535)
                     /gene="escN"
                     /locus_tag="N7C74_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HGU2434337.1"
                     /translation="MISEHDSVLEKYPRIQKVLNSTVPALSLNSSTRYEGKIINIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHVSGMYCGQWLSYQGDEF
                     KIRVGDALLGRLVDGIGRPMESNIAAPYLPFERSLYAEPPDPLLRQVIDQPFILGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEYFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVTSEHLRAAAECKKLIATYKN
                     VELLIRIGEYTMGQDPEADKAIKNRKLIQNFIQQSTKDISSYEKTIESLFKVVA"
     gene            complement(1519..>3300)
                     /gene="escV"
                     /locus_tag="N7C74_004629"
     CDS             complement(1519..>3300)
                     /gene="escV"
                     /locus_tag="N7C74_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HGU2434338.1"
                     /translation="LSVSTTRLILLHHDAGDIIYSFGNFVVGGNIVVGLVIFTIITIV
                     QFMVITKGAERVAEVSARFSLDGMPGKQMSIDGDMRAGVIDPLEAKVLRSRVQKESQF
                     YGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGMWQFDMPFSAALSLFSVLSVGDALV
                     AQIPALIISVTAGVVVTRVPGESEKEENLAGDIVQQVSVNSRPFLISAALMLVMAIIP
                     GFPALVFLFLAVCLLGIAWKLQKKRTFGTGNNKDAMGADLSNSQNISPGAEPLILNLS
                     SNIYSSDITQQIEVMRWNFFEESGIPLPKIIVNPVKNNDSAIEFLLYQESIYKDTLID
                     DTVYFEAGHAEISFEFVQEKLSTNSIVYKTNKTNQQLAHLTGMDVYATTNDKITFLLK
                     KLVLSNAKEFIGVQETRYLMDIMERKYNELVKELQRQLGLSKIVDILQRLVEENVSIR
                     DLRTIFETLIFWSTKEKDVVILCEYVRIALRRHILGRYSVSGTLLNVWLIGSDIENEL
                     RESIRQTSSGSYLNISPERTEQIIGFLKNIMNPTGNGVILTALDIRRYVKKMIEGSFP
                     SVPVLSFQEVGNNIELKVLGTVNDFRA"
BASE COUNT          991 a          800 c          605 g          904 t
ORIGIN      
        1 atacaacgac actttatcca aattcaccag cgatgcacaa tatgcctgct tagaggcaat
       61 cgactcttta atggattgct cgcagtcatc gagtttacca tccacctctt ttatctgaga
      121 gttaatctta gccattgatt ctctcaatcg gttcgctctg cttttacgaa tagataaaat
      181 tctgtccaac atactcaggc aaccactttg aataggcttt caatcgtttt ttcgtaacta
      241 ctgatatctt tggtgctttg ttgtataaag ttctgaatta atttcctatt ttttattgcc
      301 ttatctgctt caggatcttg ccccatcgtg tactcaccaa tacgaataag cagctcaaca
      361 tttttataag ttgcaataag ctttttgcat tctgccgccg cacgcaaatg ctccgatgta
      421 acaacgttat gcataacacg actggcactt aaaccaatat caattgcagg gaaatggttt
      481 tcctctgcaa gttctcgggt aagtacgatg tgcccatcaa gaatagagcg gacttcatcg
      541 ccaataggat cattaacatt atcgctttct aacaacaccg tataaatcgc ggttattgaa
      601 ccttttggcg caggccccgc tcgctctaat aatttgggta acgaagaaaa aacactcgga
      661 gggaatcccc cccttacatc aggttctccc gatgcaagcc caacatcacg tgcggctcgg
      721 gcataacgag ttacagaatc catcattaat aatacatttt taccttgatc gcggaagtac
      781 tctgcaatag tcgtcgccgt aaatgcggct ttcatccttt ccagcgcggg gcggtctgac
      841 gttgtgacga ccagtacaca tttagaaagc gtggattgag gtaagagcgc gaggaattca
      901 tttacttcgc gaccacgttc gccgataaga gcaaggacaa taatatctgc ggatgcgcca
      961 ttacaaatca tccccaaaag cgtactttta ccaacgcctg aaccagcaaa aataccgata
     1021 cgctgcccaa taccgcaagt tagtagccca tcaattgccc tgacgccaag tataaatggc
     1081 tgatcaataa cctgccttaa taaaggatct ggtggttcag catacaagct gcgttcaaac
     1141 ggaagatagg gggcagcaat attactctcc attggtctgc ctatgccatc aacaagccgc
     1201 ccaagtagtg cgtctccaac acgaatcttg aactcatctc cctgataact taaccactga
     1261 ccacagtaca tgccggaaac atgctcaaaa ggcaacaaga aaacctcatc ttcatcaata
     1321 gcgatcacct cggctaaacg ttgactgggt tctatcttat aaaaggcgcc aatacgcgct
     1381 tttggtaaac gcgccttaat aatcgtcccg ccgatattga taatcttgcc ttcatatctg
     1441 gtagacgaat ttaatgataa tgccggcaca gtgctattga gcactttctg aatacgtggg
     1501 tatttttcca atacagaatc atgctctgaa atcatttacc gttcctaata ctttaagttc
     1561 gatattattc ccaacctcct gaaaagagag cacggggact gacgggaacg aaccttcaat
     1621 cattttcttc acatagcgcc tgatatctaa agcggtcaga atgacgccgt tccccgttgg
     1681 attcatgata ttttttaaga agccaattat ctgctcagtt cgctccggtg agatattcag
     1741 atacgaacct gatgacgttt gtctgatcga ctctcgtagc tcattttcaa tatcagagcc
     1801 aataagccaa acgttcagaa gtgtaccgct aacgctatag cgacctaaaa tatgccgacg
     1861 cagggcaata cgaacgtatt cgcacaagat aaccacatcc ttttcttttg ttgaccaaaa
     1921 aataagcgtc tcaaagatag ttctcaggtc tctaattgag acattttcct ctacgagacg
     1981 ttgtaggatg tcaacaattt tgctcaaacc aagctggcgc tgcagctctt tcacaagctc
     2041 gttatatttt ctctccatga tgtccatcaa ataacgcgtt tcttgtacgc cgatgaactc
     2101 tttggcatta gacaatacaa gtttctttag caaaaacgtt atcttatcat ttgttgttgc
     2161 ataaacatcc atacctgtaa ggtgagcgag ctgttgatta gtcttattcg tcttatatac
     2221 gatagagtta gtcgaaagct tttcctggac aaattcgaat gatatctctg catgcccagc
     2281 ctcaaaatag acagtatcat ctataagagt atctttgtat attgactctt gatagagcaa
     2341 aaattctatt gcgctatcat tatttttaac cggattaaca ataatcttag gcaatggaat
     2401 tccactttcc tcaaagaaat tccaacgcat gacctcaatt tgctgtgtaa tgtctgaact
     2461 ataaatatta ctacttaagt ttaaaatcaa tggctcagcg cccggtgaga tgttttggct
     2521 attagacaaa tcagctccca tagcatcctt attattgcca gttccaaatg ttcttttctt
     2581 ctgtaatttc caggctatcc ccaacaggca aaccgccaga aataagaaga ccaatgcagg
     2641 aaagcccgga ataatcgcca ttacgagcat tagtgcggca ctgattaaaa aagggcgact
     2701 attaacagaa acctgttgaa caatatcacc tgcaaggttt tcttcttttt cgctttcacc
     2761 gggcacacga gtaacaacca cgcctgcggt gacagaaata ataagcgcag ggatctgggc
     2821 aaccaaagca tcaccgacag acaatacaga aaacaggcta agtgccgcac taaatggcat
     2881 gtcaaattgc cacataccaa tgagcacgcc accaaaaagg ttaactaaaa caataatgat
     2941 accagcaata gcgtcccctt ttacaaactt catcgccccg tccattgaac cataaaactg
     3001 gctttccttt tgcactcggg agcgaagaac ttttgcctca agcgggtcaa taacgcctgc
     3061 gcgcatatca ccatcaatac tcatttgctt tcctggcata ccatcaaggg aaaaacgagc
     3121 actcacttca gcaacacgct cggccccttt tgtaataacc ataaattgaa caatggtgat
     3181 aatagtaaaa ataaccaggc caacaacaat attaccgcca acaacaaagt taccgaagga
     3241 gtaaataatg tcacccgcat cgtgatgcag cagaataagt ctggttgtac taacactgag
//
LOCUS       DBFIFM010000314         3295 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.315, whole genome shotgun sequence.
ACCESSION   DBFIFM010000314 DBFIFM010000000
VERSION     DBFIFM010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.315"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            278..1798
                     /locus_tag="N7C74_004630"
     CDS             278..1798
                     /locus_tag="N7C74_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HGU2434339.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLVADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSTTVKQRVMAARERQFKRQNKLNAWLDSPEIRKFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(1822..2160)
                     /gene="maoP"
                     /locus_tag="N7C74_004631"
     CDS             complement(1822..2160)
                     /gene="maoP"
                     /locus_tag="N7C74_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HGU2434340.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            2279..3118
                     /gene="hdfR"
                     /locus_tag="N7C74_004632"
     CDS             2279..3118
                     /gene="hdfR"
                     /locus_tag="N7C74_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HGU2434341.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(3214..3289)
                     /locus_tag="N7C74_004633"
     tRNA            complement(3214..3289)
                     /locus_tag="N7C74_004633"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3254..3256),aa:Trp,seq:cca)
BASE COUNT          888 a          784 c          805 g          818 t
ORIGIN      
        1 ttttggccgt tttttatgat ggctttaata atttcaatag aaattgttgt tttttaaaaa
       61 gaaaatagtg agtgaaaaaa attcaatttc agtacgaatg ttgttccctc cctcgtagtt
      121 catcctttgt ccgctgcatt tttgcgagca tcattccacc gccagaacaa ctcaacttca
      181 cttgcacaaa taaacaggaa tcagccacta aaattcacga attagcgggc tacagcagcg
      241 atatcttatg cacataatgg tgactcaagg agggcttatg tcactgtcaa ttgttcatac
      301 ccgcgcagcc ctgggagtaa atgcgccccc aatcactgtt gaggtacata tcagtaaagg
      361 tttgcccggc ttaacgatgg tgggcttacc agaaacaacg gtaaaagaag ctcgcgatcg
      421 cgtgcgcagc gccattatca atagcggata tgaatatccg gcgaaaaaaa tcaccattaa
      481 ccttgctcca gccgatctac caaaagaagg gggacgatat gatttaccta tcgccattgc
      541 gttgctggcg gcctcagaac agcttacagc caataagtta gatgaatatg aattagtcgg
      601 agaactggcg cttacaggcg ctctgcgtgg cgttcccggt gcaatctcca gtgcaactga
      661 agctattaag tcgggcagaa aaattatcgt cgcgaaagat aacgaagatg aagtggggct
      721 aattaacggt gaaggatgcc tggtagccga tcatctgcaa gctgtctgtg cgtttctgga
      781 aggtaagcac gctctcgaac gcccgaaacc aactgatgca gtatcccggg cgctacaaca
      841 tgatctcagt gatgttgtcg gtcaggagca aggaaagcga ggactggaaa ttaccgccgc
      901 tggcgggcac aaccttttac tgattgggcc gccgggaaca ggtaaaacaa tgctcgccag
      961 ccgtattaat ggtcttttgc cagatttaag caatgaagag gcactggaga gcgctgcgat
     1021 attaagtctg gtaaatgctg aatcagtaca aaaacaatgg cggcagcgcc cgttccgctc
     1081 acctcatcac agtgcgtcgt taacggcgat ggtgggcggt ggtgcaattc cagggccagg
     1141 tgaaatttcg ttggcgcata acggcgtgct ttttcttgat gagctacctg aatttgaacg
     1201 gcgtacactg gatgctttgc gagagccgat tgaatccggg cagatccatc tttcacgcac
     1261 acgagcaaaa ataacctatc cagcccgttt ccagcttgtt gcggcgatga atcccagccc
     1321 taccggacat tatcagggaa accataaccg ctgcacgcca gaacagacat tacgttatct
     1381 caaccggctc tcggggccct ttctcgaccg cttcgatctc tcactggaga tcccattacc
     1441 accccccggc attttgagta aaacggtagt gccgggagaa agcagcacca ccgttaaaca
     1501 acgtgtaatg gctgccagag agcgccaatt taagcggcag aataagttga acgcctggct
     1561 ggatagtccg gaaatacgca aattctgcaa gcttgagagc gaagatgcgc agtggctgga
     1621 agaaacgctg atccatctgg ggttatcgat tcgtgcctgg cagcggttat tgaaagttgc
     1681 acgaaccatt gctgatattg atcagtctga cattatcaca cgtcagcatt tgcaggaggc
     1741 agttagctat cgtgcgattg accgtttgct catccatctg caaaaactac tgacataaaa
     1801 aagggcattt cgcccttttt attaatcgtc agaatcggtg tagtcttcag caccttcaac
     1861 ctgcggttta ccgccggaaa gggtgtgaaa acgttttgga cgcttgatac gcgtcatata
     1921 cttggaccac acgcgttctg cttctgtcac tggctcacgt tcgccacggc atactgctac
     1981 gaagagtttc tcttcctcgg taaccggctc gcgtttgcca agatccaact cattgaaggc
     2041 ataaccatga cgctcaagca gttgtgcctc tttgatggtg aaatcaccat ggcgagagaa
     2101 tccacgtgga taatgtttat tgtcgaaata tcgattagtc gtcgtaaagc tttccgccat
     2161 cctgcacgct cctaattctt tgaccgagct agttatggcg cggagtatta gttacgcttg
     2221 acagagtgta aaacaaaaca tttaaatcat aacgacaaat aattttgcgg agagcactgt
     2281 ggatacggaa ttgttaaaaa ctttcctgga agttagccga acgcgtcact ttggtcgagc
     2341 ggctgaatcg ctctatctga cccagtcagc agtgagcttt cgaatcagac aactggaaaa
     2401 tcaactgggt gtgaaccttt tcacccgcca cagaaacaat atccgtttaa ccgctgccgg
     2461 tgaaaaacta ctgccttatg cagaaacgct catgagcacc tggcaggccg cccgtaagga
     2521 ggtggcgcat acctcacgac ataacgagtt ttctatcggt gccagcgcct cattgtggga
     2581 atgtatgctt aatcagtggc tgggacgttt gtatcaaaat caggatgccc atacaggctt
     2641 acagttcgaa gcgcgaattg cccaacggca gtctctggta aaacagctgc atgaacgcca
     2701 gcttgatctt cttattacca ctgaagcgcc caaaatggac gaatttagta gtcagttgct
     2761 ggggtatttc actttagcgc tttataccag tgccccttct aaactaaagg gagatcttaa
     2821 ctatctgcga ctggagtggg ggccagattt tcaacagcat gaggcaggtt tgatcggtgc
     2881 tgacgaagtg cccattctga caaccagttc tgctgaactg gcacagcaac agattgcgat
     2941 gcttaatggt tgcacctggc tacccgtcag ctgggcgcgt aaaaaaggcg gcctgcatac
     3001 cgttgtcgat agcacaacac tttcacggcc gctttatgcc atatggctgc aaaatagcga
     3061 taaaaatacg ttgattcgcg atctattgaa aattaacgtg ctggatgaag tgtattaatc
     3121 tgaatggctg gcaaggatgc cggtagaagg atttacttca gagagggtta tttcagataa
     3181 aaaaaatcct tagctttcgc taaggatgat ttctggcagg ggcggagaga ctcgaactcc
     3241 caacacccgg ttttggagac cggtgctcta ccaattgaac tacgccccta attag
//
LOCUS       DBFIFM010000315         3230 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.316, whole genome shotgun sequence.
ACCESSION   DBFIFM010000315 DBFIFM010000000
VERSION     DBFIFM010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.316"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(1..361)
                     /locus_tag="N7C74_004634"
                     /pseudo
     CDS             complement(1..361)
                     /locus_tag="N7C74_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(361..777)
                     /locus_tag="N7C74_004635"
                     /pseudo
     CDS             complement(361..777)
                     /locus_tag="N7C74_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(777..962)
                     /locus_tag="N7C74_004636"
     CDS             complement(777..962)
                     /locus_tag="N7C74_004636"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434342.1"
                     /translation="MDVRNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGGMY
                     CPECGTVHWPDGVPPPF"
     gene            1139..>3230
                     /gene="katP"
                     /locus_tag="N7C74_004637"
     CDS             1139..>3230
                     /gene="katP"
                     /locus_tag="N7C74_004637"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase KatP"
                     /protein_id="HGU2434343.1"
                     /translation="MIKKTLPVLILLALSGSFSTAVAADKKETQNFYYPETLDLTPLR
                     LHSPESNPWGADFDYATRFQQLDMEALKKDIKDLLTTSQDWWPADYGHYGPFFIRMAW
                     HGAGTYRTYDGRGGASGGQQRFEPLNSWPDNVNLDKARRLLWPVKKKYGSSISWGDLM
                     VLTGNVALESMGFKTLGFAGGREDDWESDLVYWGPDNKPLADNRDKNGKLQKPLAATQ
                     MGLIYVNPEGPGGKPDPLASAKDIREAFSRMAMDDEETVALIAGGHTFGKAHGAASPE
                     KCIGAGPDGAPVEEQGLGWKNKCGTGNGKYTITSGLEGAWSTSPTQFTMQYLKNLYKY
                     EWELHKSPAGAYQWKPKKAANIVQDAHDPSVLHPLMMFTTDIALKVDPEYKKITTRFL
                     NDPKAFEQAFARAWFKLTHRDMGPAARYLGNEVPAESFIWQDPLPAADYTMIDGKDIK
                     SLKEQVMDLGIPASELIKTAWASASTFRVTDYRGGNNGARIRLQPEINWEVNEPEKLK
                     KVLASLTSLQREFNKKQSDGKKVSLADLIVLSGNAAIEDAARKAGVELEIPFTPGRTD
                     ASQEQTDVASFSVLEPTADGFRNYYSKSRSHISPVESLIDKASQLDLTVPEMTALLGG
                     LRVMDINTNNSSLGVFTDTPGVLDNKFFVNLLDMSTRWSKADKEDTYNGFDRKTGALK
                     WKASSVDLIFSSNPE"
BASE COUNT          856 a          746 c          826 g          802 t
ORIGIN      
        1 agttaccttt acgcgacatt gatgttttca gaccaaactg ctcctgtatg acccggtaat
       61 cgtatgcgca gtactgtgaa cctcgatcag agtggtggat tagcccggca ggtgggcgct
      121 ggctcctgag cgccataaac agggctttac ctgtcagctc ttttgtcatg cgctctccca
      181 tggcgtagcc gacaatttcg cacgtataaa catctttgat gccagcgagg tacaaccatc
      241 cctcctgtgt ggcaacatac gtcaggtccg ccacccagac ctgatttggt gctgcaggag
      301 cgaacgtctg gttcagcaga tttggcgcaa ctggcagatt gtggttcgag ttcgtagtcg
      361 cacatctgcc tggcggcaga tttcctgtat cacatccgca gcaatgcggc tcatctctgc
      421 cagtaaccac cggttgtgga acaccaggga ccagtactgg cagggaagtg tgaacacaat
      481 atgctgccac ggacagtcgg gaaccagact cagcagatac tgtatccact gtgcgccagc
      541 cttcactccg cagtgcgggc aggagcgact tttacaccgg aagcagacct tttttatgtg
      601 gctgcagtcc ggagatgaac agcaccactg tgtgtacccc atcagcgtgg tcccgcaggc
      661 catgattttt gtcacagact caatgactac cggacgcact gaaccttccg gttgtttctc
      721 cagccagtta agccagcggt ttccctgctg aaaaatgtcg gcaaaacggg gaagcatcag
      781 aagggcgggg gaactccgtc cggccagtga accgtgccac actccgggca gtacatgccg
      841 ccggcgctga taccggcaag aatggtcgca aactcccgct ccgtgcagcg ggctatttca
      901 ggataccctt cgtcatcaac acgtacaaac cagaagacca gctttttgtt tctgacatcc
      961 acaaagaagg gaatattcag gtctgcgcag cactcaacgg catcgtcagt tgcggcttgg
     1021 aaccccttag tattttttgt ctgtagtatc tatcccagca ataggtatat cctgttgcat
     1081 caataaagtt gacttttgta tacaacatgc gaatttccct taatccggag ctattcgtat
     1141 gataaaaaaa actcttcctg ttctgattct tctggcgcta tcggggagct tttctaccgc
     1201 tgtagccgct gataaaaaag agactcaaaa tttctactat ccagaaacac tggatttaac
     1261 tcctctgaga ttacacagcc ctgaatcaaa tccctggggg gctgattttg attatgccac
     1321 cagatttcaa cagctggata tggaggctct gaaaaaagat atcaaagatt tgctgacaac
     1381 ttcccaggat tggtggcctg cggattatgg tcattatggt cctttcttta ttcgtatggc
     1441 ttggcacggt gccggaacat acaggacata tgatggccgg ggaggcgcca gtggtggtca
     1501 gcaacgtttt gaaccgctga acagctggcc ggataacgtt aatctggata aagcccgtcg
     1561 attgctgtgg ccagtcaaga aaaaatacgg ctccagtatt tcctggggag acctgatggt
     1621 cctgactggt aatgttgccc ttgaatccat gggatttaaa acgctgggat ttgctggcgg
     1681 aagagaagat gactgggagt cggacctggt atactggggg cctgacaaca agcctcttgc
     1741 agataaccgg gataaaaacg ggaaacttca gaaacctctt gccgccacgc agatgggact
     1801 tatttatgtc aatcctgaag gccccggtgg aaaaccagat cctctggctt ccgcgaaaga
     1861 tatcagggaa gctttttcac gtatggccat ggatgatgag gagactgtgg ccctgatcgc
     1921 gggagggcat acatttggta aagcacatgg tgcagcgtct cctgaaaaat gtattggcgc
     1981 agggcctgat ggtgcacctg tggaggagca gggactggga tggaaaaata aatgtggtac
     2041 aggaaacggc aaatatacca tcaccagtgg cctggaagga gcctggtcga catcgccaac
     2101 ccagttcaca atgcagtatc tgaagaattt atataaatat gaatgggagc tgcacaagag
     2161 tcctgccggt gcttatcagt ggaagcctaa aaaagcggca aatatagttc aggacgcgca
     2221 tgatccgtct gtcctgcatc cgttgatgat gtttacgacg gatattgctc ttaaagttga
     2281 tcctgaatat aagaaaataa ccacccgttt cctgaatgat ccaaaagctt ttgagcaggc
     2341 attcgcaaga gcatggttta aactgaccca ccgggatatg ggaccggcag cccgatatct
     2401 tggtaatgaa gttcctgcag aatcatttat ctggcaggat cctcttcctg cggcggatta
     2461 tacaatgatt gatggtaaag acattaagtc gctgaaagag caggttatgg atttgggtat
     2521 ccctgcatct gagctgataa agacagcctg ggcttcagct tccacatttc gtgtgactga
     2581 ttatcgtggg ggaaataatg gtgcccgcat caggttacag cccgaaatta actgggaagt
     2641 taatgagcct gaaaaactga agaaagtact ggcatccctg acctcattac agcgtgaatt
     2701 taacaaaaaa cagtctgacg gaaagaaagt gtcgttggct gatttaattg ttctttcggg
     2761 taatgctgca atcgaagatg cggccagaaa agccggggtg gaacttgaga ttccctttac
     2821 tccgggaaga actgacgcct ctcaggagca gacggatgtt gcctcattca gtgtactgga
     2881 gccgacagca gatggattca gaaattatta ctcaaaaagc agaagtcata tatcgccggt
     2941 tgaaagcctc attgataaag ccagtcagct ggatctcacc gttcctgaaa tgacggcatt
     3001 actgggtggt ctgcgggtaa tggatattaa tacaaataat tcttcgttgg gagtgtttac
     3061 cgatacccct ggtgttctgg ataacaagtt ttttgttaat ctgctggata tgtcaacacg
     3121 atggagtaaa gcagataaag aagatacata caatggattc gatcgtaaaa cgggagcatt
     3181 aaaatggaaa gcatcctctg ttgatttaat cttcagttca aatcctgaat
//
LOCUS       DBFIFM010000316         3229 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.317, whole genome shotgun sequence.
ACCESSION   DBFIFM010000316 DBFIFM010000000
VERSION     DBFIFM010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.317"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..335)
                     /gene="pliG"
                     /locus_tag="N7C74_004638"
     CDS             complement(<1..335)
                     /gene="pliG"
                     /locus_tag="N7C74_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HGU2434344.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVL"
     gene            406..564
                     /locus_tag="N7C74_004639"
     CDS             406..564
                     /locus_tag="N7C74_004639"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434345.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            576..869
                     /locus_tag="N7C74_004640"
     CDS             576..869
                     /locus_tag="N7C74_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HGU2434346.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            941..1600
                     /locus_tag="N7C74_004641"
     CDS             941..1600
                     /locus_tag="N7C74_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HGU2434347.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1692..2138
                     /locus_tag="N7C74_004642"
     CDS             1692..2138
                     /locus_tag="N7C74_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HGU2434348.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2345..>3229)
                     /gene="hlyE"
                     /locus_tag="N7C74_004643"
     CDS             complement(2345..>3229)
                     /gene="hlyE"
                     /locus_tag="N7C74_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HGU2434349.1"
                     /translation="VEVVKNAIETADGALDLYNKYLDQVIPWQTFDETIKELSRFKQE
                     YSQAASVLVGNIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFDEYNEKKASA
                     QKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEKSSYFQSQ
                     VDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQSFFTTLSN
                     TVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAANKMINTC
                     NEYQKRHGKKTLFEVPEV"
BASE COUNT          843 a          701 c          767 g          918 t
ORIGIN      
        1 agcactcgca gctcatattt gccagaagca gggagtgagt attgtccgtg actatcgagt
       61 tcaggggaat atctggataa atcaacagaa tcatcgattc ctggcccgaa gaggtaagta
      121 tcagcgcctt cattagagat actcacatgc actttctgac cttttttggc atagaaggta
      181 tatgtatcgt aatcgtatcc ctttatttcg ccggagtact gagcgctgct atggcctttt
      241 ctgaactcaa cattgacatt cttacccgca gcaaagctcg tggaggttag cagtgcaagc
      301 agcaatacag ccttactgat gctcttgatt ttcattggta gtcttctgtt cagaatgagt
      361 acgatgaact gttataatat aacaatccct tacgggagaa ttccagtggc tttcctgcgt
      421 tcggtaaaat cccgtcgcgg catttttgcc ggggggatta cttataaagg ggcatggact
      481 caacaaaccg tagaaattta cagctggctg gcttgtggcg cgggtttcat tgatgttagc
      541 ttatgccaaa acctggcatc ttaaagagta aaagtatgtt ttgtgtgatt tatcgaagca
      601 gcaagcgtga ccagacctat ttatatgtcg aaaaaaaaga cgatttttcg cgtgttcctg
      661 aggaactgat gaaaggtttt ggtcagcctc agttagcgat gattctgccg ctggatgggc
      721 ggaagaaact ggtcaatgcc gatattgaaa aggttaaaca ggcattaacc gagcaaggtt
      781 actatttgca gttaccgcca ccacccgaag atttgctgaa gcaacatctt tccgtcatgg
      841 ggcagaaaac agacgacact aacaaataac cgatatccgg cggtggcatt atctttgtcg
      901 gcgcgggttt tcatatccac gataaggtga ggggaacgtt atgtatcaac atcacaactg
      961 gcaaggtgcg ctgctggatt atccggtgag taaagtagtt tgtgttggca gtaactatgc
     1021 caaacatatt aaagagatgg gcagcgcagt gcccgaagag ccagtgctgt ttattaaacc
     1081 agaaacggca ctgtgcgatc tgcggcagcc attggcgatc ccatccgatt tcggttcagt
     1141 tcatcatgaa gtcgaactag cggtgttgat tggcgcgacg ctgcgtcagg ctacggaaga
     1201 gcatgtccgc aaagccattg ccggttacgg cgtggcgctc gatctgacgt tgcgtgatgt
     1261 tcagggaaaa atgaagaaag ccgggcagcc gtgggaaaag gctaaagcgt ttgataactc
     1321 ttgtccgctt tccgggttta ttcccgcggc ggaattcacc ggcgatccgc aaaatacaac
     1381 gctgggcctg agcgtaaacg gcgaacaacg ccagcaaggt acgactgcgg acatgatcca
     1441 taaaatcgtt ccgctgatcg cttatatgag caagtttttt accctcaagg ccggtgacgt
     1501 tgtgctgaca ggcacgcctg atggcgtcgg cccgttgcaa agcggtgatg agctgacagt
     1561 cactttcgat gggcattctt tgacaactcg cgttttgtaa tactttttgc cgcctgaaag
     1621 cggcggcaaa acttgcatcg ctgtgccaga ctggttataa ggtgcgtttt aacgtaatgg
     1681 cggaacacct gatgagcgat gtacctttct ggcaaagtaa aaccctggac gaaatgagcg
     1741 atgcggaatg ggagtcgttg tgtgatggtt gcggtcagtg ttgcctgcat aaactgatgg
     1801 atgaagacac cgacgaaatc tacttcacta acgtcgcctg tcgccagctc aatattaaaa
     1861 cctgtcaatg tcggaactac gaacgtcgtt tcgagtttga acccgactgc attaaattaa
     1921 cccgtgaaaa tctgccaaca ttcgaatggc taccaatgac ctgcgcttat cgtttgctgg
     1981 cggaaggtaa agatttacct gcgtggcatc cgctgcttac tggttcgaaa gcggcaatgc
     2041 atggtgaacg tatctctgtg cgtcatatcg cagtgaaaga atcagaagtg attgactggc
     2101 aggatcatat cttaaataaa cctgactggg cgcagtgata aatttaatag atgttgatgg
     2161 gggctttctt aatatacgtc tggatgctcg ccatttcgta gccctgtttt tgaaagcgct
     2221 ctgtcataat gagagttacc cgatacctta ttatcaacag gtaactctca atctttaaaa
     2281 aacgtctgta tttttaaatg tgataaactt tataccttga gtacatggag agaataattg
     2341 ctgatcagac ttcaggtacc tcaaagagtg tcttcttacc gtgtcttttc tgatactcat
     2401 tacaggtgtt aatcatttta ttggccgctt cttttagcaa agaaagcatt aaatcatcat
     2461 aatcaacata gaatctggtt gtttcagttt ccgtttttat ctccccgatg gcggctattt
     2521 cggtggttaa tttcaatttg gcggcatcga tatctttatt cgcttgttta accgtgttag
     2581 acagggtggt aaagaaactc tgcacagatt ttaacttgtt cttcaattct ggaatcagtt
     2641 tcccttcaac tacgcccgca gcaatagaat aggaaatgat taaaccaaat ggaccggcga
     2701 cgacaccggc tgcggcaccg gcatacgctt ccttcctgat tttatctacc tgtgactgga
     2761 aatagctgct tttttctgaa aaatcattgg ttaactggct atctaacgcc agcagtttcc
     2821 cggaagcgtt gttgaaactt tgtgagctta ccagcaggga tttttgcgct tcattcagct
     2881 tcgtgatgcc gtcatccagt accttaatga gaatgtcttt ctgggcggat gctttcttct
     2941 cattatactc atcaaatagc aaaatatacg ctgcgagcaa ttgcgtcgca acaccacacc
     3001 attcatacac tgtttgggtt gcttcaaaat acttatcctg gctatccatt agtaaggttt
     3061 taatattgcc aactaaaacg gaggctgcct gtgaatactc ctgtttaaag cgacttaact
     3121 cttttatggt ttcatcaaag gtctgccagg ggatgacctg atcgagatat ttattataaa
     3181 gatccaatgc tccatctgcg gtttcgattg cgtttttaac tacctctac
//
LOCUS       DBFIFM010000317         3224 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.318, whole genome shotgun sequence.
ACCESSION   DBFIFM010000317 DBFIFM010000000
VERSION     DBFIFM010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.318"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(94..621)
                     /gene="mobB"
                     /locus_tag="N7C74_004644"
     CDS             complement(94..621)
                     /gene="mobB"
                     /locus_tag="N7C74_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HGU2434350.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQNQDG"
     gene            complement(603..1187)
                     /gene="mobA"
                     /locus_tag="N7C74_004645"
     CDS             complement(603..1187)
                     /gene="mobA"
                     /locus_tag="N7C74_004645"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HGU2434351.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIAMVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1257..1526
                     /locus_tag="N7C74_004646"
     CDS             1257..1526
                     /locus_tag="N7C74_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HGU2434352.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1603..2589
                     /gene="srkA"
                     /locus_tag="N7C74_004647"
     CDS             1603..2589
                     /gene="srkA"
                     /locus_tag="N7C74_004647"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HGU2434353.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2606..>3224
                     /gene="dsbA"
                     /locus_tag="N7C74_004648"
     CDS             2606..>3224
                     /gene="dsbA"
                     /locus_tag="N7C74_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HGU2434354.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSE"
BASE COUNT          813 a          780 c          792 g          839 t
ORIGIN      
        1 aagcgaaagg ccatcctgac ggatggcctt tttgcgttgg tgcaaaaaaa tgccggatgc
       61 gacgctggcg cgtctgctcc agcctactct tgcttatccg tcctgatttt gcatccactc
      121 caccacaaaa tcagccagcc cctcaacatc attaatatcc agtaatgcga catcaagatt
      181 aagcggcaca tcactggcta cagcaataac atgcctgtct atcactaatt cttcaggtcg
      241 atgtccggct ccatcgcgaa acagcacaat ctttgcgatc tcttcatgct taaacccttc
      301 gaccagaatc aaatccagct ttgaggtatc cattcgactt gcgagaaact gtagatccag
      361 ctcttcttcg tctggtgttt ccgtcatcaa ggcccatcgt tgctggctgg caacgatggt
      421 ttgtgccgcg ccagccttgc gcagctcata gctatctttg cctggcttat caacatccat
      481 atcatggtgc gtatgcttaa tcagccctgg acggatccct ctggcgcata atgccgggat
      541 cagttttttc aaaagcgtag tttttccggt accactccac gcggcaaagg cgagtaacgg
      601 tatcatcgtt tttcctgcca tcgggcaagc tcctctggcg tattcacgtt aacaaatgca
      661 tctttatgat cgctgaaatc aaccgcatga ccgccagcca gacgcataaa tgccattacc
      721 cggcgttctc ctgcttgcag atattctagt aataaaggct caatagcgcg gtttaccata
      781 gcaatagtcg ggtgatcgcg ttcaccgtca tggacccaca cgacaggcgc atctttgcgc
      841 tgatgattaa gccgggcggc taaatcatgg ggaatgtaag gcgtatcgca cggacaaaac
      901 aaaaaccact caccagcttc ctgctgcatt actgaaagca tccctgccag agggcctggg
      961 taatccgcca gtgaatcttc aatcactttc agaccgcctg cctgatagat ttcctgatga
     1021 cgattagcat taaccacgac gtgagagagc tgcgtcataa gcgcgtcagc gacatgttgc
     1081 cacagcggtt tgccatttaa gtccagcaat cctttatcta cgccgcccat tcgtctggct
     1141 ttaccgcctg ccagcacaac gcctgttatc gtcgtcatca gattcaccga tatcgcctct
     1201 tttattgtgg gattgaccct gctaacgtgt ctgtctcaag agtaaggagc attactatga
     1261 aatgtaaacg tctgaatgaa gttattgaac tcctccagcc agcctggcaa aaagagccag
     1321 accttaacct gttgcaattt ttgcagaaac tggcgaaaga gtcaggtttt gacggcgaac
     1381 tggcggattt gacggatgac attctgatct atcacctgaa aatgcgtgat tccgcgaaag
     1441 atgcggtgat cccgggtttg cagaaagatt atgaagaaga cttcaaaacg gcgctgttac
     1501 gcgctcgcgg cgtaattaaa gagtaaaagc ttgtaagcgg cgccaccaaa atcatcgtga
     1561 aatgatatcc ttcatcattc gtaatgtttt ccggatgatg ggatgaacaa cagcgctttt
     1621 actttccaga cactacaccc ggataccatc atggacgctc tgtttgagca agggatccgg
     1681 gtggattccg gtcttacccc gcttaacagc tatgaaaacc gtgtctatca atttcaggac
     1741 gaagatcgtc gacgttttgt cgtcaaattt tatcgtcctg aacgttggac agccgatcaa
     1801 atcctcgaag aacatcaatt tgcgttgcag ctggtaaatg atgaagttcc ggttgcagca
     1861 cctgtggctt ttaacggtca gactttattg aatcatcagg gattttattt cgctgttttt
     1921 ccaagcgtcg gtggtcgcca gttcgaagct gataatatcg atcagatgga agcggttggg
     1981 cgttatttag ggcgtatgca ccagacgggg cgcaaacagc tttttatcca tcgcccgacc
     2041 atcggtctga atgaatatct cattgagcca cgcaagctgt ttgaggacgc tacactgata
     2101 ccttccgggt tgaaagcggc attcctgaaa gcgacagatg agctgatcgc cgccgttaca
     2161 gcacactggc gggaagattt caccgttctg cggctacatg gagactgcca cgccgggaat
     2221 attctctggc gcgatggtcc aatgtttgtt gatctggatg atgcacgtaa tggtccagct
     2281 attcaggatt tgtggatgtt gctcaatggc gataaagccg agcagcggat gcaactggaa
     2341 actattattg aagcttatga agaatttagc gagttcgaca ccgctgaaat cggactgatt
     2401 gaacctttac gcgccatgcg tttggtttat tatcttgcct ggctaatgcg gcgttgggct
     2461 gatcccgcgt tcccgaaaaa tttcccgtgg ttaaccgggg aagattactg gctacgacag
     2521 acggcgactt ttatagaaca ggcaaaagtt ctacaagaac cccctttgca attaacacct
     2581 atgtattaat cggagagagt agatcatgaa aaagatttgg ctggcgctgg ctggtttagt
     2641 tttagcgttt agcgcatcgg cggcgcagta tgaagatggt aaacagtaca ctaccctgga
     2701 aaaaccggta gctggcgcgc cgcaagtgct ggagtttttc tctttcttct gcccgcactg
     2761 ctatcagttt gaagaagttc tgcatatttc tgataatgtg aagaaaaaac tgccggaagg
     2821 cgtgaagatg actaaatacc acgtcaactt catgggtggt gacctgggca aagatctgac
     2881 tcaggcatgg gctgtggcga tggcgctggg cgtggaagac aaagtgactg ttccgctgtt
     2941 tgaaggcgta cagaaaaccc agaccattcg ttcagcatct gatatccgcg atgtatttat
     3001 caacgcaggt attaaaggtg aagagtacga cgcggcgtgg aacagcttcg tggtgaaatc
     3061 tctggtcgct cagcaggaaa aagctgcagc tgacgtgcaa ttgcgtggtg ttccggcgat
     3121 gtttgttaac ggtaaatatc agctgaatcc gcagggtatg gataccagca atatggatgt
     3181 ttttgttcag cagtatgctg atacagtgaa atatctgtcc gaga
//
LOCUS       DBFIFM010000318         3221 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.319, whole genome shotgun sequence.
ACCESSION   DBFIFM010000318 DBFIFM010000000
VERSION     DBFIFM010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.319"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..659
                     /locus_tag="N7C74_004649"
     CDS             <1..659
                     /locus_tag="N7C74_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434355.1"
                     /translation="VFAHMRGEVAAADDDDLLSNKLKQIREIRLAGLEVIHVIHRHGM
                     TDEKTAYEVEAALIDAYPGLTNIMNGAGSNEFGAAHVKELIATYQPETITFHHKALMI
                     SVNRSAKDSELYDAVRFSWRINVSRASKAEVILATVRGIVRGVFIADKWLKSTREHFP
                     TMKYWDEDPDFEATQSSRYGFEGREAPPEIANLYLGKKIPDELRKKGAMSPVRYSPNF
                     "
     gene            complement(717..1271)
                     /gene="pinE"
                     /locus_tag="N7C74_004650"
     CDS             complement(717..1271)
                     /gene="pinE"
                     /locus_tag="N7C74_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
                     /protein_id="HGU2434356.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVILVEELRERGVNFRSLTDAIDTSTPMGR
                     FFFHVMGALAEMERELIVERTKAGLEAARAQGRIGGRRPKLTPEQWAQAGRLIAAGIP
                     RQKVAIIYDVGVSTLYKKFPAGDK"
     gene            1301..1711
                     /locus_tag="N7C74_004651"
     CDS             1301..1711
                     /locus_tag="N7C74_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2434357.1"
                     /translation="MPFARYFCIFINVGLGEAAKRDVGTGENQIPDMASFASGNGWMK
                     LPNGKILQYGRGAVTPTLSTQTMRITFSIPFPKKADCAMLTHSGDGGAPLGAGRGFVM
                     TAEGPTLTGFNSAYRTSSTSDTVSMNYSWWAVGE"
     gene            1732..2175
                     /locus_tag="N7C74_004652"
     CDS             1732..2175
                     /locus_tag="N7C74_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU2434358.1"
                     /translation="MNEYVYSARHNAFFPVDMIDKYKSEGWDLSDAKEVNQNIISEFM
                     AEPPQGKIRIAGDDGLPAWADIPPPTHEELIEITESERQLLINQANEYMNSKQWPGKA
                     AIGRLKGEELAQYNLWLDYLDALELVDTASAPDIEWPTPPAVQAR"
     gene            complement(2147..2749)
                     /locus_tag="N7C74_004653"
     CDS             complement(2147..2749)
                     /locus_tag="N7C74_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU2434359.1"
                     /translation="MNNAELNSELIATMAGEITVYNFDVMSREYISASTEYLAIGVGI
                     PAYSCLDAPGAYKAGYAICRSADFNSWEYVPDHRGEIVFSTETGESKEITAPGDYPDN
                     TTTIAPLTPYDKWDGEKWVTDTEAQHSAAVEAAETQRQSLIDTAMASISLIQLKLQAG
                     RKLTQAETNRLNTVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(2749..>3221)
                     /locus_tag="N7C74_004654"
     CDS             complement(2749..>3221)
                     /locus_tag="N7C74_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001486660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2434360.1"
                     /translation="LPVGVPVPWPSATPPTGWLKCNGAAFSAEEYPELAKVYPTNKLP
                     DLRGEFIRGWDDGRGVDNGRGLLTLQDGAIVSHNHYWGIWTSRTNDQTLESFTGTTIL
                     KQITPLSPAINFDNYPIPNPAITEGGVVAATTKPAGANETRPRNVAFNYIVRAA"
BASE COUNT          860 a          751 c          763 g          847 t
ORIGIN      
        1 gcgtatttgc ccatatgcgc ggtgaagtgg cagcggctga tgatgacgac ttactgagca
       61 acaagctaaa gcaaatcaga gaaataagat tagcgggact tgaagttatc catgtcatcc
      121 atcgacatgg aatgactgat gaaaagacgg cgtacgaagt tgaagcagca cttattgatg
      181 cctaccctgg gttaacgaat atcatgaatg gtgctggcag caatgaattc ggcgccgcgc
      241 atgtcaaaga gttgatagca acatatcaac ccgaaaccat aacatttcat cataaagcat
      301 taatgatttc cgttaacaga agtgcaaaag attcagagct ttatgatgcg gttcgattta
      361 gctggcgcat taatgtctcg cgcgccagta aagcagaagt cattcttgct actgtaaggg
      421 ggatcgttcg aggagttttc attgctgata aatggctaaa atcaacgcgt gaacattttc
      481 ctacgatgaa atactgggac gaagaccctg actttgaggc aacacaaagc tctcgctatg
      541 gttttgaagg tcgagaagcc ccacctgaaa tagcaaacct ttatcttggg aaaaaaatac
      601 cagatgaatt aagaaaaaaa ggagctatgt ctccagttcg ttactcacct aatttttgaa
      661 tcttcaagtg gtaagcataa accgcagcac gatcttgcat acgacgtgct acggtttcat
      721 ttatctccgg ccggaaactt cttatacagt gtcgatacac caacatcata gatgatcgct
      781 accttctggc gaggaattcc tgctgcaatt aatcgtccgg cctgcgccca ttgttctggt
      841 gtaagtttgg gacgacgtcc accaattcgt ccctgtgcgc gagcagcttc cagtccagct
      901 tttgttcgtt caacaatcag ttctcgttcc atttcagcca gggcccccat cacatgaaag
      961 aaaaagcgcc ccattggggt actggtatca atagcatccg tcaggctgcg aaaattaacg
     1021 ccacgttcgc gcaactcctc aaccagaatg accagatgcc gcatactgcg ccccagccga
     1081 tccagtttcc agacaaccag agtgtcacct gccgataatg tcctgagcag ttttttcagt
     1141 cccggccttt cggactttgt accgcttatc ttgtcttcaa aaatcagctc gcatcctgca
     1201 cagttcagcg cattacgttg tagatctgtg ttctggtcat ttgttgacac acgtacatag
     1261 ccaataagca tggtagatct ccctgacaaa agcaggaatg atgccatttg ctcgttattt
     1321 ctgcattttc ataaacgttg gtttgggaga agcggcaaaa cgggatgtgg ggacagggga
     1381 aaatcagata ccggacatgg cctcttttgc cagtggtaat ggatggatga aattacccaa
     1441 cgggaaaatc ctgcaatatg gtcgtggtgc ggttacgccg acattatcga cgcaaacaat
     1501 gagaattaca ttcagcatcc ctttccccaa aaaagcggac tgcgccatgc ttactcattc
     1561 tggtgatggc ggtgcgcctt taggcgctgg gcgagggttc gtgatgactg cagaaggccc
     1621 aacgttaacc ggctttaatt ctgcttacag aacgtcatca accagcgaca cggtatcgat
     1681 gaattacagt tggtgggctg ttggtgagta attttattca gggtgattta tatgaacgaa
     1741 tatgtttata gcgcaaggca taatgctttt ttccctgtgg atatgattga taaatataaa
     1801 tcagagggat gggatttatc agacgctaag gaagtaaatc aaaatattat cagtgagttt
     1861 atggctgaac cgccacaagg aaaaatccgt attgccggag atgatgggct gcctgcgtgg
     1921 gcagatattc ctccacccac gcatgaagaa cttattgaaa ttactgaatc agaaagacag
     1981 ctactaatta accaggccaa cgaatacatg aacagtaagc aatggcccgg taaagccgct
     2041 attggtcgtc tgaaaggtga ggaactggca caatataatt tgtggctgga ttatctggac
     2101 gcactggagc tggtcgatac cgccagtgcg ccagatattg aatggcctac gcctccggca
     2161 gttcaggcca gatgacatcc ggcgcggtgc tggtatctgt tgccgtcacc gcgtcaatgt
     2221 aatccagcac cgtgttaagt cggttggttt ctgcctgcgt cagcttccgt ccggcctgta
     2281 atttcagctg aatcagacta atggaagcca ttgcagtatc aatcagcgac tggcgttgtg
     2341 tttctgccgc ttctactgcg gcgctatgct gtgcttcggt atccgtcacc catttctcac
     2401 catcccattt atcgtatggc gttaacgggg cgatagtggt tgtattatca gggtaatcac
     2461 ccggagcggt gatttctttt gattctcctg tttcggtgct aaagacgatt tcaccgcgat
     2521 ggtctggcac atattcccat gagttaaaat ctgcagaacg gcagattgca taaccagctt
     2581 tgtatgcgcc tggggcatct aaacaggaat atgccggaat gccgacacca atagcaagat
     2641 attcagttga agctgaaata tattcccgac tcatgacatc aaagttataa acagtaattt
     2701 ctcctgccat agtggcaatt aattcactgt ttaattctgc gttattcatt atgcagccct
     2761 cacaatatag ttaaaagcga catttcgtgg gcgtgtttca ttcgcacctg caggtttagt
     2821 cgttgccgca acaacaccac cctctgtaat agccgggttg ggaattgggt aattatcgaa
     2881 gttaatggcc ggagacaggg gcgtgatttg ttttaaaatc gtggtgcctg taaaactttc
     2941 cagagtctgg tcgttagttc gtgaagtcca gattccccaa tagtggttat gactgacaat
     3001 cgcaccgtcc tgaagcgtca ataatcccct tccgttatcc actccacgtc cgtcatccca
     3061 gccacgaata aactcaccac gtaaatcagg caatttattt gtcgggtaaa cctttgccag
     3121 ttccgggtat tcttcagcag aaaaagccgc cccgttgcat ttcagccagc ctgtcggcgg
     3181 agtggctgaa ggccacggaa caggcacacc aacgggtaat g
//
LOCUS       DBFIFM010000319         3205 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.321, whole genome shotgun sequence.
ACCESSION   DBFIFM010000319 DBFIFM010000000
VERSION     DBFIFM010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.321"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            39..833
                     /gene="hyxR"
                     /locus_tag="N7C74_004655"
                     /pseudo
     CDS             39..833
                     /gene="hyxR"
                     /locus_tag="N7C74_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
     gene            987..1748
                     /gene="hyxA"
                     /locus_tag="N7C74_004656"
     CDS             987..1748
                     /gene="hyxA"
                     /locus_tag="N7C74_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HGU2434361.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIGTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VEHVISIANSSGGLSGLKHAENLPVMDSYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LEKTNAMTLRENGNTNRYSVVVNAGTQVLSLHKYK"
     gene            1790..2872
                     /locus_tag="N7C74_004657"
                     /pseudo
     CDS             1790..2872
                     /locus_tag="N7C74_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798070.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            2862..3050
                     /locus_tag="N7C74_004658"
     CDS             2862..3050
                     /locus_tag="N7C74_004658"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU2434362.1"
                     /translation="MLWLRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTK
                     GDDIENPLQGVVGINVTW"
BASE COUNT          882 a          678 c          803 g          842 t
ORIGIN      
        1 aatgtcaggt tttgtagcta tggcgtggac gagtctgaat gaagaagcct ctggttatta
       61 tcagtgcctg tcagtttacc aggctggcac tggagtctct catcccagcg gacaggtata
      121 tcgtgcgggt gtacagcaac gtcactacag aggttgaatt cgttttaaag acctcctgtg
      181 gctatctgtt ggctgatatc ccttagcttc cttcggggga gttagttctt ctggcttgtc
      241 ttgtccgctt gcggagtgat gccgggcaat ggcaggtctg cctgaccggt gactccgact
      301 ggttcgataa tgctcctgct gcaagtctgt ataacggctt cccacgcatc ggcaccatgc
      361 tgacagcgag tcgttatcag aatcaaatta tccgctggtt actacgtcct ttgcccggcg
      421 atatgagcga tttctggctc ctgactgcac gggagttgag cgtgcttaag attctgatgc
      481 agggcatgag tttcagtgag attgcccgtt atgaacaacg cagttcaaag accctgcatg
      541 cgattgccac acgggcatta atgaaacttg gactgggaac tctgagcgat ttcaggctgc
      601 tctatacggg atgcggtgac agcagagtga aacggaatat acggctacat gcaagatatg
      661 tcggacaaca ttccgctcat cgactcactc agtatgtgca gaacatggta tccggaattc
      721 tttatccaga gaaagttgcc gttggtgaat caataaattc tgacgccatt tccggaaaag
      781 tacttcccgg acaaagaaaa aatatttcta aaaaaagacc aggattgtta tgacacaaca
      841 gcgttgttac taccccccac tgcggcgcgt acggactcgc tacagtgtag gggccaaaat
      901 atttctactg ctctgggctg caggattggg aggcacgctg tacagcctgt acagcgtgct
      961 ggcgtactga catctgtgag ggaaggatgc gtttcgttct tttctgtccc tcaggaattg
     1021 taccagcgca gttcgcagct ctatcaaccg gttctgtcgg caatgttacc tgcatcggaa
     1081 cagaagagga gttacgcaat aaattgcggc acaggccaca gagtgtcgtg atatcggcag
     1141 ggcgacctgc cgaatgcgct gaaatgtggt tccgatttta tcgggatcac tcattcgtgg
     1201 tggtgttgtg cgtcgcgcca ttttttctgc caccggatgt gagtatttct ggcgttctca
     1261 aaaacctgag gttactgaaa ccaggaatgt cagtagagca tgtcatcagt attgcaaatt
     1321 cctctggtgg gttatcaggg ctgaaacatg cagaaaattt gccagttatg gacagttact
     1381 ctgtctttat gaaagaggtg aataaccgca caaagacaat cgtcatgtca gaacgcttcc
     1441 cggaaaagca aaagaaggtg ttgtcgttgc ttcttgccgg acacagctgg gaatacagtg
     1501 cgcagtttct caaaacgggg ataagacaga tatggcttgc cgagcagtcg ctaaaaaagc
     1561 gttggggaat accggacagc atgtcattgc gggaagccct gctgctgagc agcaataaat
     1621 ttcacggtga tggaaatgca ttggaaaaaa cgaatgccat gacattgcgt gaaaacggaa
     1681 ataccaacag gtattccgtg gtggtaaatg cagggacgca ggttttatct ctgcataaat
     1741 ataaataagt gggaaattga aattagttac cagaatggag aatttcttca tgaaaaacag
     1801 taaggcattt taccgcagcg cattagcgac agctattgtt atggctcttt ctgcaccagc
     1861 attcgctgct gataacgcgg tatcaactga ttcggttacg ctgaataaag ataagacgac
     1921 tctggatcaa gatgttgtta ttagcaatac agcagacaaa cagattacag ccgtaacaat
     1981 taatgcggca gatgaagacc ttaatgttgc ttttgctggt catgatatta ctgctgaatc
     2041 aacggcagac aaaaaattcg ttgaaggtgt aaaagttagt ggtaacaaaa atgttgtgat
     2101 taatgctaca gactccacca tcacagctca aggtgaaggc acctatgtcc ggactgcaat
     2161 ggtcattgat tcaactggcg atgttgttgt taatggcggt aatttcgttg caaaaaatga
     2221 aaaaggtagt gcgacaggga tatctctgga aggggccaag ggaaataatg tcacgcttaa
     2281 tggcacaacc ataaatgctc aaggtaataa gagttccagc aacgcctcca cggcaatttt
     2341 tgctcaaaag ggtagcttat tgaatagttt tgaaggtgat gcaaccgaca acattaccct
     2401 tgctggctca aatattatta atggtcagat tgaaacaata gttactgccg ggaataagac
     2461 gggaattcat acagtcaacc tgaatattaa ggatggctca gtaattgggg cggctaataa
     2521 taaacaaaca atttatgcct ctgcttcggc acaaggcaca ggttcagcaa cgcaaaattt
     2581 aaatttgtct gttgctgatt caaccatcta ctctgatgtc ctggcccttt ctgaaagcga
     2641 gaattcagcc gctaccacaa caaatgtaaa catgaacgtt gcccgctctt actgggaagg
     2701 taatgcttat actttcaata gcggcgataa agcgggtagt aatctggata taaatctttc
     2761 cgatagttca gtctggaaag gcaaagtttc aggggcagga aatgccagtg tatctctgca
     2821 aaacgagtct gtctggaatg ttacgggatc ctcaactgtt gatgctctgg ctccgtctgg
     2881 gtgatgagtc tgtcaatgcc agcattgacg gtgcagcatt ccgcgtgggt gcaggtgtac
     2941 aggctgatat caccaaaaac atgggagcat atgcaagcct tgactacacc aaaggtgacg
     3001 acattgagaa tccgctacag ggtgtagttg gtatcaatgt gacctggtaa gaataaagca
     3061 gtcggttccc ctgtcgactg ttataccccc tgcaggcagc agtatgctgc ctgcaggcta
     3121 taaatatcca gtattcatga tgctggcttt ttgctgatat ttaacccgaa cattcctgat
     3181 atagactatt aattcattta tctgc
//
LOCUS       DBFIFM010000320         3203 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.322, whole genome shotgun sequence.
ACCESSION   DBFIFM010000320 DBFIFM010000000
VERSION     DBFIFM010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.322"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..129
                     /locus_tag="N7C74_004659"
     CDS             <1..129
                     /locus_tag="N7C74_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU2434363.1"
                     /translation="RIPAQRWGRAQDLMGAMVFLASAASDYVNGQLLVIDGGYSIR"
     gene            complement(275..964)
                     /locus_tag="N7C74_004660"
     CDS             complement(275..964)
                     /locus_tag="N7C74_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU2434364.1"
                     /translation="MKSVLLLLTILFVQSVEAGIMPASSRIIYRDGDREKTLMLVNTN
                     DYPVLVQSWVDNGEGSPEAASPPFVVLPPVFRLAARRTQGLRIIYNHDPLPQDRESAF
                     WLNLYEMPPETPHQDPTHLTLAMNTQLKIFYRPKSVTMTPQEAMRKISWHLGNDGSSS
                     YIELNNPTPLNISFTSISIGATKVQQESDMMIKPFSQRRYWLASNNVSGVVAATYLDD
                     NGAQIKQQLAQ"
     gene            complement(961..2295)
                     /locus_tag="N7C74_004661"
     CDS             complement(961..2295)
                     /locus_tag="N7C74_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2434365.1"
                     /translation="MSKSTFLHILISSIILVALIQSSAWANCTNTQIGQTEDGRTALI
                     EFGKINMTDTYFAPAGSLLATTVVPPTNYTSGGATGSSVLWECDATDLPNIYFLVATN
                     GDDRVGGFYDAGGPDGLSDVYATWFAFVGLKQTMAGVTLGRYWKKVPITSYATQGTKI
                     QIRLQDIPPLHAELYRISTLPDTSATTSWCGNNNTDSSGVGFAKPSGTIYNCVQPNAY
                     IQLSGTSGILFGHDEPGEDSSVHWDFWGADNGFGYGMRSANRLYNNATCVARSATPLV
                     LLPTIAEAQLNAGMESTGNFNVRVECSNSVQSGISDTQTALGIQVSEGAYTAAQKLGI
                     INSNGGVSALVSDNYDAAEMAKGVGIYISNSAHPDTAMTLVGQPGIAKLTPGGNAAGW
                     YPVFEGATLEGATHPGYSSYSYSFIARLKKLPNQTVSAGKVRATAYILVKMQ"
     gene            complement(2311..>3203)
                     /locus_tag="N7C74_004662"
     CDS             complement(2311..>3203)
                     /locus_tag="N7C74_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU2434366.1"
                     /translation="VGRQRMGGYKDNSDDMQTVTSLSFSFPLGGNGPRVPSLSNSWTH
                     STDGSSQLQSSLTGMLDEAQTTNYSLNVMRDQQYKQTTLSGNMQKRFSQTTVGLNASK
                     GQDYWQASGNVQGAMAVHGGGITFGPYLGETFALVEAKGAEGAKVYNSSQLEINDSGY
                     ALVPAVTPYRYNRISLDPQGMDGDAELVDSERQVAPVAGAAVKVIFRTRPGKALLIKS
                     RMADGSELPMGADVLDENNTVVGIAGQGGQIYLRTEQTKGHLSVRWGEGANDSCQLPF
                     DISGKDSNSPIIRLNETCQS"
BASE COUNT          764 a          788 c          776 g          875 t
ORIGIN      
        1 cgtataccgg ctcaacgctg gggaagggca caggatctta tgggcgcaat ggtgttcctc
       61 gccagtgccg cttcagatta tgtcaatgga caactattag tcattgatgg tggatattca
      121 attcgttaac gatttttggc tgcgttgagg tgttttgact ggaaaatgga gtggagagat
      181 tttgtcattt ctccacttcc ggtacttcag gtgggtatag tggttattcg ttagcgttga
      241 accatcgcga tgagtttaca taccactcag agcattattg cgctaattgt tgttttatct
      301 gtgcaccatt atcatcgaga tatgtcgccg caaccacacc actaacgtta ttacttgcta
      361 accagtaccg tcgttgactg aagggtttta tcatcatatc gctttcttgc tgcacctttg
      421 tagccccaat gctgatggag gtgaaagaga tattcagtgg tgttgggtta ttaagctcaa
      481 tatagctact gctgccgtcg ttacccagat gccaacttat tttacgcata gcttcttggg
      541 gcgtcatagt tacagatttt ggcctgtaaa agattttaag ttgcgtattc attgccagcg
      601 ttaaatgtgt gggatcttga tggggagttt ctggcggcat ttcataaaga tttaaccaga
      661 atgcagattc gcgatcctgg ggaagtgggt catgattata gataatccgc aacccctggg
      721 ttcgtctcgc agccaggcgg aaaaccggtg gcagcaccac aaagggggga gaagcagctt
      781 ctggggaacc ttcaccatta tctacccaac tttgcaccag taccggatag tcattggtat
      841 tgaccagcat cagtgtcttt tcccgatcgc catcgcgata aataatccgt gaagaagccg
      901 gcatgatgcc ggcttccact gactgtacga acaagatggt taagagcagc aaaacggatt
      961 tcattgcatt ttcaccaata tataagccgt tgcccgcact tttcccgcac tgactgtctg
     1021 atttggcagt ttcttcaacc gggcgataaa agagtaacta tagctggagt atccggggtg
     1081 agtcgcacct tctaatgttg ccccttcaaa tacaggatac caccccgctg catttcctcc
     1141 gggggttaac ttcgcgatgc ccggttgacc aaccagcgtc atcgccgtat cggggtgagc
     1201 actgttagaa atgtagatcc caacgccctt tgccatctct gctgcgtcat aattatcaga
     1261 gaccagggcg ctgacgccgc cgttgctatt gataatcccc agtttttgcg ccgctgtata
     1321 tgcaccttca gacacctgga ttcctaatgc tgtctgagta tcgctaatcc ctgattgaac
     1381 cgagttacta cactcgacgc ggacattaaa attaccggta ctttccatgc ccgcattcag
     1441 ttgtgcttct gcaattgtcg gcagcaatac taacggcgtg gcgctgcggg caacgcaggt
     1501 ggcattgttg tagagtcgat tggccgaacg cattccgtaa ccaaaaccat tatcagcacc
     1561 ccagaaatcc caatgaacag aactatcttc gccgggctca tcatgaccaa ataaaatgcc
     1621 gctggtaccg gaaagctgaa tataggcatt gggctgaaca cagttataga ttgtaccgga
     1681 aggttttgcg aatccgactc cactactatc tgtattatta ttaccgcacc aacttgttgt
     1741 tgctgatgta tcaggtagcg tactgatgcg ataaagctca gcatgaagag gagggatatc
     1801 ctgtaagcga atctggattt tagttccctg agtggcataa ctggtgatgg gcactttctt
     1861 ccagtaacga ccaagcgtca cgcccgccat ggtctgcttg agaccgacaa aagcgaacca
     1921 ggtggcatag acatcactca gaccatcagg cccgcctgca tcgtaaaagc cccccacgcg
     1981 gtcgtcgcca ttggtagcga ccagaaaata gatgtttggc aaatctgttg catcacattc
     2041 ccacaatacg gagcttcctg tcgcgccacc tgatgtgtag ttagtgggag gtacgaccgt
     2101 tgtcgcgagg agtgaacctg ctggcgcaaa ataggtgtcg gtcatattaa tttttccgaa
     2161 ctcaataagc gctgtccgac catcttcagt ttgacctatc tgtgtgtttg tacagttagc
     2221 ccaagcggat gattgtatta gtgccaccaa tatgatggag ctaatcagaa tgtgtaaaaa
     2281 agtcgattta gacatcgggt tttctctgta tcaagactga caggtttcat tcaggcggat
     2341 gatagggcta ttgctgtcct tcccgctgat atcaaagggc aattggcagc tatcgttagc
     2401 accttcaccc cagcgaactg acaagtggcc ttttgtctgt tctgtgcgga ggtaaatttg
     2461 ccccccctga ccggctatac cgacgactgt attattctca tccagcacat cggctcccat
     2521 tggcagttcc gaaccatctg ccatgcggga tttaatcagc aacgctttac caggacgggt
     2581 acggaaaatt actttcaccg ccgcacccgc aaccggtgct acctgtcttt cactgtcgac
     2641 caactcggca tcgccatcca ttccttgtgg atcgagagat atacggttgt agcgataggg
     2701 cgttactgcc ggaacaagcg catagccact gtcattaatt tccagctgac tggagttata
     2761 gacttttgca ccttctgcgc ctttagcttc gaccagggcg aacgtttcac ccagataagg
     2821 tccgaaagta atgccgccac catgcacagc catcgcgcct tgtacgttac ctgaagcctg
     2881 ccagtaatcc tggcccttcg atgcgttcaa tccgacggta gtttgtgaaa aacgtttttg
     2941 catgtttccg ctaagcgtcg tctgcttata ttgttgatcg cgcatgacgt tcaggctgta
     3001 gttggtggtc tgtgcttcat caagcattcc ggttagcgag ctttgtaatt gcgagctacc
     3061 gtcagttgaa tgggtccagc tgttgctaag acttggtaca cgaggtccat tgccgccaag
     3121 tgggaatgag aatgaaaggg atgttaccgt ctgcatatca tcagaattgt ctttatagcc
     3181 gcccattctt tggcgtccga cgg
//
LOCUS       DBFIFM010000321         3189 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.323, whole genome shotgun sequence.
ACCESSION   DBFIFM010000321 DBFIFM010000000
VERSION     DBFIFM010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.323"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..176
                     /locus_tag="N7C74_004663"
     CDS             <1..176
                     /locus_tag="N7C74_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU2434367.1"
                     /translation="EGINFNQNNHVVIQNDGSNGQAGPQLMKAVYDMARKGAQDEIQA
                     QMRDGGVFSGGRR"
     gene            173..502
                     /locus_tag="N7C74_004664"
     CDS             173..502
                     /locus_tag="N7C74_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2434368.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            502..1200
                     /locus_tag="N7C74_004665"
     CDS             502..1200
                     /locus_tag="N7C74_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU2434369.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTAQGGERYFFCNELNE
                     KGEAVTWQGRQYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            1211..1954
                     /locus_tag="N7C74_004666"
     CDS             1211..1954
                     /locus_tag="N7C74_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU2434370.1"
                     /translation="MTQTESAILVHARRCAPAESCGFVIGTPEGERYQPCVNISAEPE
                     AYFRIAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFEREDDWWRNGQNLYLDNMAAT
                     GFYRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGICNDLAAASACT"
     gene            1951..2529
                     /locus_tag="N7C74_004667"
     CDS             1951..2529
                     /locus_tag="N7C74_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU2434371.1"
                     /translation="MNTAAEAIRALSLQMPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEPLGEGAVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGATMALWGAALSAGGLT
                     ATTMLFSLGASMILGGVAQMLAPKAKTPEYRATDNGKQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
     gene            2770..>3189
                     /locus_tag="N7C74_004668"
     CDS             2770..>3189
                     /locus_tag="N7C74_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2434372.1"
                     /translation="MGKGGGKGHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGSETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVQTTSQGDRNPASVRLLIQ"
BASE COUNT          688 a          777 c         1084 g          640 t
ORIGIN      
        1 cggaaggcat taattttaat cagaacaatc acgtggtgat tcagaacgac ggctccaacg
       61 gacaggcggg gccgcagctg atgaaggcgg tgtatgacat ggcccgcaag ggggcgcagg
      121 atgagattca ggcgcagatg cgtgatggcg gcgtcttttc cggaggcagg cgatgaaaac
      181 ctttcgctgg aaagtgaagc cggatatgga ggtgaactcg cagccatcgg tgcgtgaagt
      241 gcgttttggt gacgggtatt cgcagcgtat ggcggcgggg ctgaatgctg acctgaaaac
      301 ataccgtgtg acgctttccg tgacccggga ggaggcccga catctggagg cattcctggc
      361 agagcacgga ggctggaagg catttttgtg gaagccaccc tatgcatacc ggcagataaa
      421 ggtgacctgt gccgggtggt ctgcgcgggt cgggatgttg cgcgttgagt tcagcgcgga
      481 gtttaagcag gtggtgaact gatgcaggat attcacgaag aaagtctgaa cgagtcggtt
      541 aaatcagagc agtcaccgcg ggtggtactc tgggaaatcg acctgacggc gcagggcggt
      601 gagcggtatt ttttctgcaa tgagctgaat gaaaaagggg aggcggttac ctggcagggg
      661 cggcaatatc aggcataccc gattgacggc agtggctttg agatgaacgg gaagggcagc
      721 agtgccagac cgtcgctgac ggtgtcgaat ctgtttggtc tggtcaccgg gatggcggag
      781 gacctgcaga gcctggtggg ggccacggtg gtccgccgcc gggtgtatgc ccgttttctg
      841 gatgcggtga attttgtggc aggcaatcct gaggcagacc ctgagcagga gctgacggac
      901 cggtgggtgg tggagcagat gtcatcgctg acggccatga cggcctcgtt tgtgctggcg
      961 acaccgacgg agacggacgg ggcgctgttt cccggtcgca tcatgctggc gaacacctgt
     1021 atgtgggatt accggggcga tgaatgcggg tataacggtc cggcagtggc ggatgagttc
     1081 gacaacccca ccacggatat ccggaaggac agatgcagta aatgcatgcg cgggtgtgag
     1141 atgcgcggca tggtggctaa ttttggcggt ttcctttcca tcaataaact ttcgcagtaa
     1201 atcctgtttt atgacacaga ctgaatcagc gattctggtg catgcccggc ggtgtgcgcc
     1261 tgcggagtcg tgcggcttcg tgataggcac cccggagggc gaacggtacc agccctgcgt
     1321 gaatatctcc gcagagccgg aggcgtattt tcgtattgcg ccggaagact ggctgcaggc
     1381 agagatgcag ggggagattg tggcgctggt tcacagccac cctggtggtc tgccctggct
     1441 gagcgaggcc gaccggcggc tgcagataaa gagtgccctg ccctggtggc tggtctgccg
     1501 gggggaaatt cataaattcc gctgtgtgcc gcacctgacc gggcgtcgtt ttgagcacgg
     1561 ggtgacggac tgttacaccc tgttccggga tgcataccat ctggcgggga taacgctgcc
     1621 ggattttgag cgtgaggatg actggtggcg caacggtcag aacctttacc tggacaatat
     1681 ggcggcgact ggtttttacc gggtgcccct gtcctctgca caggcgggcg atatcctgct
     1741 gtgctgcttt ggcgcatcgg tggccaatca tgccgccata tactgcggca acggtgaact
     1801 gcttcaccat ctgcctgaac aactgagtaa acgggagagg tattctgaaa aatggcaacg
     1861 acgaacgcat tctgtctggc gtcaccgcca ctggcacgca tctgccttca cggggatttg
     1921 caacgatttg gccgccgcct cagcctgtac gtgaacacgg cagcggaagc catccgtgcc
     1981 ctgtcgctgc agatgccggg attccgccgt cagatgaacg aaggctggta ccagatacgt
     2041 attcgcggtg aggacacggc accggaggcg gtgtacgccc gtcttcacga acctctgggt
     2101 gagggggcgg tcatccatat tgtgccgcga ctggccggag ccggaaaggg cggactgcag
     2161 attgtgctgg gggcagcagc catcgtgggc tctttcttca ccgccggcgc aacgatggcg
     2221 ttgtggggcg cagccctgag tgccggaggg ctgactgcca ccacgatgct gttctcactg
     2281 ggtgccagca tgatactggg cggtgtggcc cagatgctgg caccgaaggc aaaaacaccg
     2341 gagtacaggg cgacggataa cggtaaacag aacacgtatt tttcgtcact ggataacatg
     2401 attgcccagg ggaacccgat gccggtgcct tatggtgaaa tgctggttgg ttcacgacgg
     2461 atatcccagg acatcagcac ccgtgatgag ggcggagacg ggaaagtggt ggttatcggg
     2521 cggggatgaa aataaaaaaa tcccgcagag ttagcggagc tgcgggagag aacgatgaag
     2581 attaacgtta tggagttatt tttcaggcat caaaaaagta atgcagcgtc attattgcgg
     2641 ctacaggcaa ttgccggaaa tgtgaagagt ttcagaaatt ttattccgtc atgacacagg
     2701 caccctccgg ggtgcctgtt gttttctggc ataaacagat tcagacatca gacaggagag
     2761 ggggacagag tgggtaaagg gggcggcaag gggcacacgc cggtagaggc aaaggacaat
     2821 cttaagtcca cgcagatgat gagcgtgatt gacgccattg gtgaagggcc gattgaaggt
     2881 ccggtgaagg ggctgcagag tattctggtg aacaaaaccc cactgacgga cacggacggc
     2941 aatcccgtga tacacggtgt gaccgcggtc tggcgcgccg gggagcagga gcagacaccg
     3001 ccggaaggtt ttgagtcatc cggctctgaa accgcactgg gcgtggaagt gacgaaggca
     3061 aagccggtga cgcgcaccat tacgtccgcg aacattgacc gcctgcgggt taccttcggg
     3121 gtgcagtcac tggtgcagac cacctcacag ggtgaccgta acccggcatc cgtccgcctg
     3181 ctgattcag
//
LOCUS       DBFIFM010000322         3185 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.324, whole genome shotgun sequence.
ACCESSION   DBFIFM010000322 DBFIFM010000000
VERSION     DBFIFM010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.324"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(24..785)
                     /gene="kduD"
                     /locus_tag="N7C74_004669"
     CDS             complement(24..785)
                     /gene="kduD"
                     /locus_tag="N7C74_004669"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HGU2434373.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(815..1651)
                     /gene="kduI"
                     /locus_tag="N7C74_004670"
     CDS             complement(815..1651)
                     /gene="kduI"
                     /locus_tag="N7C74_004670"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HGU2434374.1"
                     /translation="MDVRQSIHSAHAKTLETQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIIGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTGTPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1938..3119)
                     /gene="yqeF"
                     /locus_tag="N7C74_004671"
     CDS             complement(1938..3119)
                     /gene="yqeF"
                     /locus_tag="N7C74_004671"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HGU2434375.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PHTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
BASE COUNT          834 a          877 c          713 g          761 t
ORIGIN      
        1 gtcataaaag taagaggaat gaattaacgc gccagccaac cgccatccac agcaatggta
       61 taaccattta cataatctga agcgctggag gcaagaaaca ctaccggccc catcaggtca
      121 ctcggcagtc cccaacggcc agctggaatg cggtcgagaa tttccgcgct acgttgttca
      181 tctgcccgca gttgttgggt attgttggtc gccatgtaac ccggagctat cgcattaaca
      241 ttaatgttgt gtttagccca ttcattcgcc atcaatcgcg tcacgcccat cacgccgctt
      301 tttgatgcgg tataagaagg cacacggatc ccgccctgga aggagagcat tgacgcgata
      361 ttgataatct tgccgccatt gccttgcgcg atgaagtgtt tcgccgctgc ctgagacatg
      421 aagaatacgc tcttgatatt caggttcatg acatcgtccc agtccttttc gctgaactcg
      481 agagcatctt cgcggcgaat caatccggcg ttattcacca ggatatcaat atgaccaaac
      541 tcagctaccg cgcgatccag cagtccagga ataccatcaa tctttcgcag atcggcggtc
      601 aggcttaaaa aacgacgccc cagcgccgtg acctgcttga tggtttcagt cggttcaacg
      661 atgttaatgc caacaatgtc acagcccgct tgcgccagcc ccaacgccat cccctggccc
      721 agcccagtat cacaaccagt gacgaccgca actttacctt cgagagaaaa tgcacttaaa
      781 atcataacaa taccttattc gtttatgccc acaactagcg caaatcttta acggccacat
      841 ggtccatatc atcaaagacc tggttttcac cgaccatgcc ccagataaag gtataagctt
      901 tggttccgac gccggaatga atcgaccagc tcggggagat caccgcctgc tcgttatgca
      961 tcacaatgtg acgcgtttct tgcggctgcc ccatcatgtg gaaaacgcag gcgtcatcat
     1021 ccatattgaa atagaaataa acttccatcc ggcgctcgtg ggtgtgacac ggcatggtgt
     1081 tccacaagtt acccggagcc agctccgtca gccccatact caattggcag gtttccagca
     1141 catccgggac aaaatatttg ttaatcgtgc gacggttact ggtgaggtta tcgcctaacg
     1201 tgactggaga tacttcgtcc ggtgtgactt ttttggtagg atacgtcgta tgcgcgggtg
     1261 cgcaattgta ataaaacttc gccggagtgc cggtatcaat actagcaaag acaacttctt
     1321 ttgcgccttt acccacatac agggcgtcgc ggtgaccgat ttcatagcat tgaccatcga
     1381 cagtaatcgt accggcaccg ccaatattga taacacctaa ctcgcgacgt tcaaggaaat
     1441 aacttacgcc gagttgttta cccacttccc cgccaacgga aaccgtttta gttaccggca
     1501 taatgccgcc aataataata cggtcaatgt ggctgtaaac catggtgtac tcatcggcga
     1561 caaatacctt ttcaaccaaa aattcattgc gcagcccctg ggtttccagc gtttttgcgt
     1621 gcgcactgtg gatgctctgt cttacgtcca catcaacctc cgataatcag tgaagcgtga
     1681 aaatagaaaa cgatagtgaa acactgtttc gttttctcta taaggggatg atatgcccat
     1741 gggatcgggt tttcaattaa aaataaaaca atgtttcatt tttaagttaa ggattaaaaa
     1801 aagtgcagtg tcgatcacga atctgtcatg agaatggaaa aatcagggag cccggaaaat
     1861 gtatgtcgac ttgccagcca tcattattca acagattaat gacggcagat atttatcggt
     1921 aaggaggaaa tgcagagcta ttcgtcacgt tcaatggtca atgccacacc ctggcccccg
     1981 ccgatacaaa gcgttgccag tcctttgcgg gcattacgtt tcaccatttc atgaaccaga
     2041 gaaaccagga ttcggcaacc ggaagcgcct atcgggtgac cgagtgcgat cgcgccacca
     2101 ttgacattga cccgacgctc atcccactca agcatcttgc caaccgaaag cgcctgtgca
     2161 gcaaacgctt cattagcctc gataagatca acatcagcca actgccagcc tacacgctcc
     2221 aggcaacggc gggtcgcata caccggcgca attcccatca atgccggatc tacaccaacg
     2281 ctggcaaatg cgcggatgcg ggccagcacg ggtaaattca acgctcgtgc tttggcttcg
     2341 ctcatcatca ttaccgcagc tgcgccatcg tttatggatg atgcattacc cgctgtcaca
     2401 gaaccgagac tatcaaatga aggatttaaa cgggctaaac cttctgcact ggcgtcagtg
     2461 tgtggctgtt catcggtatc aacaaccaac gtctgaccgt tactttgggt cattaccggg
     2521 acgatctcat ctttaaatcg tccggcgtca atcgccgctc gcgctttttg ttgcgagcta
     2581 agtgcgtaag catcctgcaa ttgacggctg atgccatatt cgcgagccag attttcggcg
     2641 gtgacaccaa tatgataatc attgaaggca tcccataacc catcatgcac aagactgtca
     2701 accagctggc tattgccaag ctgtgcaccg gtgcggctat cagtcagaac atgtggtgcg
     2761 cggctcatgt tttcctggcc accggcgatg acaatatcag cctcgccaca ctgtatcgcc
     2821 tgagtagcca gatgcagtgc tttaagcccg gaaccgcaga cgtcattaat cgtgattgca
     2881 gaaacgctat taggcagacc acctttaata gccgattgcc ttgccggatt ctgccctgcc
     2941 cctgcagtca acacctgacc aagaattact tcatccaccg catatgcagg aacgccggta
     3001 cgttctatta acgctttcac gaccagacta ccaagttcca cggcggaatg acccgctaac
     3061 gcaccgcgaa agcagccgat aggtgtccgt aacgccccga caatcacaac gtctttcatc
     3121 acaacctcgc accaattaac agtgcaatag taaatgattg ttaattacag ttatcttaat
     3181 tgttt
//
LOCUS       DBFIFM010000323         3148 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.325, whole genome shotgun sequence.
ACCESSION   DBFIFM010000323 DBFIFM010000000
VERSION     DBFIFM010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.325"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            583..789
                     /locus_tag="N7C74_004672"
     CDS             583..789
                     /locus_tag="N7C74_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HGU2434376.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            865..1161
                     /locus_tag="N7C74_004673"
     CDS             865..1161
                     /locus_tag="N7C74_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HGU2434377.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            1167..1952
                     /gene="bet"
                     /locus_tag="N7C74_004674"
     CDS             1167..1952
                     /gene="bet"
                     /locus_tag="N7C74_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HGU2434378.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            1949..2626
                     /locus_tag="N7C74_004675"
     CDS             1949..2626
                     /locus_tag="N7C74_004675"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HGU2434379.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEIHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVYGEQW"
     gene            2626..2808
                     /locus_tag="N7C74_004676"
     CDS             2626..2808
                     /locus_tag="N7C74_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HGU2434380.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLN"
     gene            2781..2972
                     /locus_tag="N7C74_004677"
     CDS             2781..2972
                     /locus_tag="N7C74_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HGU2434381.1"
                     /translation="MHKASPVELRTSIGMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMAAKAEANERDPA"
BASE COUNT          878 a          793 c          770 g          707 t
ORIGIN      
        1 ataaaaattg gtgaagtgga aagatataaa gaaccaattc ctatctctgc tctaggtagt
       61 aatattaagc caccacaatc atatctttac ctacctgcgt aagaatcccg gccaccgtgc
      121 cgggttttct tttgcccctc cccctcatca cacaaaccat tcaaaaaacc accacgacct
      181 cgcttcagtt atcgctatgc gatgcaagtc acaaaataaa tccatcctaa atacaaccat
      241 ttacatctaa aaaaaccaat aaaacaactt ttgttgttga cgacaaaaca actatagttt
      301 taaataaatt catcgcaaca acacaacgat acggcaacca cctgattcac cgttgcgatg
      361 accgcttaga tccgcagctt gaatttcagc aggctccggg aagtgcgagg ggtgaagtgg
      421 acgcgtgaac gtcggtgtga ccagctgaaa tcaactcaac acctcatacc tcagtcgctt
      481 caacgaggcg gcttagttat gacaaccggc ggccatccac cgcctgaata cgcgcagaag
      541 tctctatatg ttcagcagcc cagcttacgg gcaggagttt ttatggttca tcaacattac
      601 ggaacgcaga ccgttaatcg cggtgcggtc atgccaggaa tgctggtcaa acacaaagat
      661 ggtacctgga ctgcatcagc taatttacgc ggacggcttt atctgcatcg cggcatcgag
      721 cgcacttata cccgtgattt gctcgtggaa gtttttctcg acggacgcgg caacggcctg
      781 aatcactaat cccctttcct gtttacctaa tcagcccggc atttcgcggg cgatatattc
      841 acagccattt tcaggagttc agccatgaac gcttattaca ttcaggatcg tcttgaggct
      901 cagagctggg cgcgtcatta ccagcagatt gcccgtgaag agaaagaggc agaactggca
      961 gacgacatgg aaaaaggtct tccacagcac ctgtttgaat cactctgcat cgatcatttg
     1021 caacgccacg gggccagcaa aaaagccatt acccgtgcgt ttgatgacga tgttgagttt
     1081 caggagcgca tggcagaaca catccggtac atggttgaaa ccattgctca ccaccaggtt
     1141 gatattgatt cagaggtata aaacggatga gtacagcact cgcaacgctg gcagggaagc
     1201 tggctgaacg tgtcggcatg gattctgtcg acccacagga actgatcacc actcttcgcc
     1261 agacggcatt taaaggtgat gccagcgatg cgcagttcat cgcattgttg atcgtcgcca
     1321 accagtacgg ccttaatccg tggacgaaag aaatttacgc cttccctgat aagcagaacg
     1381 gcatcgttcc ggtggtgggc gttgatggct ggtcccgcat catcaacgaa aaccagcagt
     1441 ttgatggcat ggactttgag caggacaatg aatcctgcac atgccggatt taccgcaagg
     1501 accgtaatca tccgatctgc gttaccgagt ggatggatga atgccgccgc gaaccattca
     1561 aaacccgcga aggcagagaa atcacggggc cgtggcagtc gcatcccaaa cggatgttac
     1621 gtcataaagc catgattcag tgtgcccgtc tcgccttcgg atttgctggt atctatgaca
     1681 aggatgaagc cgagcgcatt gtcgaaaata ccgcatacac tgcagaacgt cagccggaac
     1741 gcgacatcac tccggttaac gatgaaacca tgcaggaaat taacactctg ctgatcgccc
     1801 tggataaaac atgggatgac gacttattgc cgctctgttc ccagatattt cgccgcgaca
     1861 ttcgtgcatc gtcagaactg acacaggccg aagcagtaaa agctcttgga ttcctgaaac
     1921 aaaaagccac tgaacagaag gtggcagcat gacaccggac attatcctgc agcgtactgg
     1981 gatcgacgtg agagctgtcg aacagggaga tgatgcgtgg cacaaattac ggctcggcgt
     2041 catcacagct tcagaaattc acaacgtaat agcaaaaccc cgatcaggaa agaagtggcc
     2101 tgacatgaaa atgtcctact tccacaccct gctggctgag gtttgcaccg gtgtggctcc
     2161 ggaagttaac gctaaggctc tggcctgggg aaaacagtac gagaacgacg ccagaaccct
     2221 ctttgagttc acttccggcg tgaatgttac tgaatccccg atcatctatc gcgacgaaag
     2281 tatgcgcacc gcctgctctc ccgatggttt atgcagtgac ggcaacggcc ttgagctgaa
     2341 atgcccgttt acctcccggg atttcatgaa gttccggctc ggtggtttcg aggccataaa
     2401 atcggcttac atggcccagg tgcagtacag catgtgggtg acacgaaaag atgcctggta
     2461 ctttgccaac tatgacccgc gcatgaagcg tgaaggcctg cattatgtcg tgattgagcg
     2521 gaatgaaaag tacatggcga gttttgacga gatggtgccg gagttcatcg aaaaaatgga
     2581 cgaggcactg gctgaaattg gttttgtata tggggagcaa tggtaatgaa gcatcctcac
     2641 gataatatcc gggtaggcac gatcactttc gtctactccg ttacaaagcg aggctgggta
     2701 tttcccggcc tttctgttat ccgaaatcca ctgaaagcac agcggctggc tgaagagata
     2761 aataataaac gaggggctgt atgcacaaag catctcctgt tgaattaaga acgagtatcg
     2821 ggatggcaca tagcctcgct caaattggag tcaggtttgt gccaatacca gtagaaacag
     2881 acgaagaatt tcatacgtta gccacatccc tttcacaaaa gctggaaatg atggcggcga
     2941 aagcagaagc aaacgagaga gacccggcat gacaacaaca gaatgcattt ttctggcagc
     3001 aggcttcata ttctgtgtgc ttatgcttgc cgacatggga cttgttcaat gacacctcag
     3061 caggaaaacg cccttcgcag cattgcccgt caggctaatt ctgaaatcaa aaaagccaga
     3121 cagcagtttc cggataaaaa cgtcgatg
//
LOCUS       DBFIFM010000324         3058 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.326, whole genome shotgun sequence.
ACCESSION   DBFIFM010000324 DBFIFM010000000
VERSION     DBFIFM010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.326"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..491
                     /locus_tag="N7C74_004678"
     CDS             <1..491
                     /locus_tag="N7C74_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434382.1"
                     /translation="SRVQQSAQQAEGNGGEDLSPVLDAMNAVPVLKTWQESDPDRFSV
                     AVSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNKADKTDIRKTAEEK
                     VKTAEQEQAVPASPSDLGTTASVGTGDNFERLLGASHSEAEAIMRGMTNAEIDALLEK
                     LG"
     gene            515..1729
                     /locus_tag="N7C74_004679"
     CDS             515..1729
                     /locus_tag="N7C74_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HGU2434383.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDR
                     AMLLGAQALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            1785..2174
                     /locus_tag="N7C74_004680"
     CDS             1785..2174
                     /locus_tag="N7C74_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434384.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSIKSGEHALIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            2224..2685
                     /locus_tag="N7C74_004681"
     CDS             2224..2685
                     /locus_tag="N7C74_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434385.1"
                     /translation="MSEKIAVVYIGPKPVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQERVLAALAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEAEELDIHKDPKETAEAFRIRVREAFRRRVAETEQHGGTE"
     gene            2669..>3058
                     /locus_tag="N7C74_004682"
     CDS             2669..>3058
                     /locus_tag="N7C74_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434386.1"
                     /translation="MAELSDFLPYVRRHISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQIPDELLTWPEEVAA"
BASE COUNT          745 a          727 c          872 g          714 t
ORIGIN      
        1 agtcccgtgt tcagcaatca gcacagcagg cagaaggtaa tggtggtgag gatttatcac
       61 cggttcttga tgcgatgaat gccgtgccgg tgctgaaaac gtggcaggag tccgatccag
      121 atcgcttctc ggttgctgta tccatcgacg ggaagctcca gaatgacccc gcatggaaag
      181 acaaaacgct cactgaacgt ttcgctgaag tggcccgtcg tacgcaggtt gctttcggtg
      241 aagtcagtga gtcgtctgct gacaacaagg cagacaaaac ggatatccgg aaaacggcgg
      301 aagagaaagt gaagaccgct gaacaggagc aggcagtacc tgcttccccg tcagatttag
      361 gcaccacggc ttccgtcgga accggtgata attttgaacg gttacttggc gcttctcatt
      421 cagaggcaga ggcgattatg cgcggtatga cgaatgctga aatagacgcg cttctggaga
      481 agctcgggta acttactgaa ggagaactga agtaatgacg actgtaacat cagcccaggc
      541 gaataagctg tatcaggtgg cgctttttac cgctgccaac cgcaaccgct cgatggtaaa
      601 tatcctcact gaacagcagg aagcgccaaa agcggtttcg ccggacaaga aaagcacgaa
      661 gcagaccagc gcgggtgcgc cggttgtccg tatcacagac cttaacaaac aggccggtga
      721 tgaagtgacc ttcagcatca tgcacaaact ctcaaaacgt ccgacgatgg gagatgagcg
      781 tgttgaaggt cgtggtgagg atctcagcca tgctgacttc tccctgaaaa tcaatcaggg
      841 acgtcacctg gtggatgcag gcggacgtat gagtcagcag cgcacgaagt ttaacctggc
      901 atcctcagcc agaacgcttc tggggacgta ctttaatgac ctgcaggacc agtgtgcgat
      961 agtgcatctt gctggagctc gtggtgattt tgttgctgac gacactattc tgccgacagc
     1021 ggagcaccct gaattcaaaa aaatcatgat caacgatgta ctgcctccga cacatgaccg
     1081 tcactttttt ggcggtgatg cgacaagctt tgagcagatt gaagcggcag atattttttc
     1141 tattggcctg gtggacaatc tctccctgtt cattgacgaa atggcgcatc cgttacagcc
     1201 ggttcgtctg tccggtgatg aacttcacgg agaagatcca tattacgtcc tgtacgtcac
     1261 gccgcgtcag tggaatgact ggtacacctc gacgtccggt aaggactgga accagatgat
     1321 ggttcgtgcc gtgaaccgtg caaaaggttt taatcatccg ctgttcaaag gtgaatgtgc
     1381 gatgtggcgc aatatcctgg ttcgtaagta tgcgggtatg ccgatccgtt tctatcaggg
     1441 gtcaaaggtt ctggtatcag agaataacct gacggcaacc acgaaagagg tcgctgctgc
     1501 aaccaatatt gaccgcgcca tgttactggg ggctcaggcg ctggcaaatg cttacggtca
     1561 gaaggcgggc ggtcacttca acatggttga gaagaaaacg gatatggata accgtactga
     1621 gatagcaatc agctggatca acggtctgaa aaaaatccgt ttccccgaga agagcggcaa
     1681 gatgcaggat cacggcgtga ttgccgttga tacagcagtg aagctctgat tttttccttt
     1741 ccctatgccg ggttttcgcc cggctttttc aggagtcatt aattatggca aagactatcc
     1801 ttgccccgtc actgagtgaa cgggtctata cgggtacgca cggtaatgag tcggtggcag
     1861 aaggcgtatt tacggtgaat gctgcggaag cggacagtgt tattcatctt ctctcactgc
     1921 cagtgggcat ccgtatcaac tcactccaac tggtttcaac gggtggtctg ggtactgcaa
     1981 ccgtcagcat taagtccggt gagcatgctc tcatcgataa cagcgaagct gtttctgcaa
     2041 aatttgccag atatgtgcca gtggagccgt acaccacaca gcgtgacggg gagctggtta
     2101 ctgtcaccat taagactgcc gctgcaaccg gcaccctgaa tgttctgctg cgttataccg
     2161 tggtgggata ctgattaaaa ccttccggcc cgcgtcatgc gggcttttta tccggggaat
     2221 tatatgagtg agaaaattgc cgttgtctat atcggcccaa aacccgtgaa aaaggacacc
     2281 attaccggaa gtcgcacgct gttcccacgt cttgagccgg tgcatgttga cagcgcgatg
     2341 gcctggcaac tgctggggtt tccggatgtc tgggttcgtc atgaagagct tgatgatgtt
     2401 ctgaaaaagc aacaacagaa tgagcagttg cggcaggcac agcaggcgca ggaaagagtg
     2461 cttgctgcgc tggcagaagc ggagaacagt tttgttgttt ctgttaacgg gcaggaggtg
     2521 gatttaagta agctcacctc agcacggctg gcgacgctgt gtgaggcaga agagctggat
     2581 attcacaaag acccgaaaga aacggctgag gcattccgta tccgggtgcg tgaggcattt
     2641 cgccgtcgtg ttgcggagac tgaacagcat ggcggaactg agtgattttt taccgtatgt
     2701 ccgtcgtcat atcagcggtc cactgaacat tatgatgacg gatgctctgt caatggctgc
     2761 cgtggcattc agccgccagt cgttggtgtg ccgtcgggag gttactgttg taccagtagc
     2821 aggaaaagaa atcgtgcttc cgtatgacaa agatgatgag gagtgcgttc atatcatccg
     2881 tatctctgac gataatcatg agctttttgt cggtcgggat gtggatatca gctccggacg
     2941 ctccctgcga tttgcctgtt ctcccggtga ggtgagcgtg ctttatgccg tcgctccgaa
     3001 agccggacgc agccagatac cggatgaact cctcacatgg cctgaagaag tggctgcg
//
LOCUS       DBFIFM010000325         3058 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.327, whole genome shotgun sequence.
ACCESSION   DBFIFM010000325 DBFIFM010000000
VERSION     DBFIFM010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.327"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            282..1514
                     /locus_tag="N7C74_004683"
     CDS             282..1514
                     /locus_tag="N7C74_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HGU2434387.1"
                     /translation="MSDTLLTEKILTGENVLRAAIARIEWIFETFPSVCLSFSGGKDS
                     TVLFHLVAEVARRRKRHFSVLFIDWEAQYRCTIEHIQKMREMYHDVTETFYWVALPLT
                     TVNGVSQFQPEWICWEPGVTWVRQPPEEAITDMTYFPFFRYAMTFEEFVPAFSSWFAG
                     NRCGVAVLTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTMYPLYDWKAR
                     DIWIYNARACAIYNPLYDLMYRADVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARM
                     CERVSGAASGALYANESGAYFALRKRISKPAHHTWRSYAMFLLDVMPERTAEHYRNKI
                     AVYLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRPLSFSPNKPRH
                     YERYLQRMKERRKEWGIL"
     gene            1499..2137
                     /locus_tag="N7C74_004684"
     CDS             1499..2137
                     /locus_tag="N7C74_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HGU2434388.1"
                     /translation="MGDSVTPEIIGNMIRQYFSQERPEEETIQALNHLRRVLHEVSPF
                     AHEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            2216..2485
                     /locus_tag="N7C74_004685"
     CDS             2216..2485
                     /locus_tag="N7C74_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU2434389.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPETLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            2506..>3058
                     /locus_tag="N7C74_004686"
     CDS             2506..>3058
                     /locus_tag="N7C74_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434390.1"
                     /translation="MMKQGMANSISMTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVA"
BASE COUNT          800 a          641 c          838 g          779 t
ORIGIN      
        1 tattacagtg aatacttcct gcaaaatgat atttgaattg ttcaccatct gcatcatatt
       61 ttcttattta caagttgaaa ggatagtata tgttgaaaat gcatggattt tccaggtgtg
      121 ttaccagtaa gaagcaggtg gataaaggat gtgataacaa atccaacaac caggttaata
      181 tctgaagatt tttgttgtta ttatttatcc ccaaagcggt gagttattaa tgaagtttgc
      241 ctgaatgtat aaacagaatt attcgacata aagccaggaa aatgagtgat acgcttttaa
      301 ccgaaaaaat ccttaccgga gaaaatgttt tgcgtgctgc aatcgcccgc attgagtgga
      361 tttttgaaac atttccgtcg gtatgtctgt ctttttcagg tggaaaggat tccaccgtgc
      421 ttttccatct tgtggcagag gtggcccgca ggagaaaacg tcatttctct gttctgttca
      481 ttgactggga ggcccagtat cggtgcacca ttgaacatat tcagaagatg cgggaaatgt
      541 accatgatgt gacggaaacc ttttactggg tggcacttcc cctgaccacg gtaaacggcg
      601 tctcgcagtt tcagccggag tggatatgct gggagccagg agtgacctgg gttcgccagc
      661 caccggaaga ggccattacg gatatgacat attttccctt tttccggtat gccatgacgt
      721 ttgaagaatt tgttccggca ttttcttcct ggtttgccgg taaccggtgt ggagtggcag
      781 tgctgaccgg tgttcgtgcg gatgaatccc tcaatcgctt tatggggctg gtgtctcagc
      841 gcaaactgag atatgccgat gataagccct ggaccacggc atcgcctgaa gggttttatt
      901 acaccatgta tccgttgtat gactggaaag cccgcgatat ctggatatat aacgccagag
      961 cttgtgccat ttacaatcct ctgtatgacc tgatgtaccg tgccgacgtg ccgttacgca
     1021 acatgcgtgt ctgtgaacct tttggtcctg aacaacgtaa ggggctgtgg ctttaccatg
     1081 ttctggagcc ggaaacctgg gccaggatgt gtgagcgggt gtcaggcgct gccagcgggg
     1141 cgctttatgc caatgaaagc ggtgcctatt ttgccctgcg taaacgtata tcaaaaccag
     1201 cccatcatac ctggcgcagc tatgcgatgt tcctgctgga tgtgatgccg gaaagaacgg
     1261 cagaacatta ccgtaataaa attgctgtct acctgcgctg gtatcagacg cggggcttcc
     1321 cggatgacat cccggatgaa caggagaatg acctggggag ccgggatatt ccgtcctggc
     1381 ggcgtatctg taagacactt ataaagaatg atttctggtg ccggcccctc tccttcagtc
     1441 cgaacaaacc ccggcactat gaacgttatc tgcagcgtat gaaagaaagg aggaaggaat
     1501 gggggattct gtgacaccgg agataattgg caacatgatt cgtcagtact tcagccagga
     1561 acgaccagaa gaagagacca tacaggccct taatcacctg cgcagagtgc ttcatgaagt
     1621 cagtcctttt gcacatgagc cagtggactg tgtgctgtgg gtaaaagcag atgaggtggt
     1681 ggccaatgat tacaatccta atgtcatggc gccgggtgag aagaaactgc tgaagcagtc
     1741 actggagaaa gatggtttca cacaaccggt ggtggtgtct gaggaaaaga accattacct
     1801 ggtggtggat ggttttcatc gccaggtgct gggcagggaa gcaggtacgg gaaaacgtct
     1861 gaagggctgg ctgccggtgg tctgtattaa tccggagaga aagggacagg cctcacgtat
     1921 tgctgcgacc atccgacata accgggccag gggcaaacat cagataacct caatgtctga
     1981 tattgtcagg gacctgtcgc gcctgggctg gacgaatgaa cgcattggta cggagctggg
     2041 tatggaccag gatgaagttc tcagattgaa acagattagc gggctgacgg agctgtttca
     2101 ggaagaggat ttcagcccgg catggacggt tcgctgagac cgtcagatat gtatattatt
     2161 tcagtgagtt aatgttttga gcaggaaaaa gagtaccccg gaagttgtgg aagacatggt
     2221 tgcgcagaaa ctggaggctg ctggttgctg gcgcagagct tctgaccgct ggctttttgt
     2281 tatgggaaat gttgaatgta cagaagcaca acgggagtgg ttgttgttgc gccggaatta
     2341 ttgtctggcg cagatatcgt ccccgccgct acctgaaacg ctggatatca gcgaggtggc
     2401 gaaagctgca gatgccacgc tgaggcgaat ggggattgcc actccgtccg gggaggtatt
     2461 cagaaaaggt acaccggtct gttgatattc aggtaacagg tattaatgat gaagcaagga
     2521 atggcaaaca gtatatcaat gacatggcaa cttttatgcg atgaacatgg ttttacccgg
     2581 gagcactaca atcagctgaa gaagttcagc ccggagacat tacgtgaaat tattgctgaa
     2641 atagccagct gtcatccatc aaccagtgta ttgctcagga ataagtggct gacaccgccg
     2701 gaggatattc tggagcagat tacccgtgaa tacgaacgaa gaatacaaaa ctgtccaccg
     2761 ttcaggagcg agaaggaagc ggaatcctgg ttaaatgaac tatcttttgc tgtgattgct
     2821 ccgctatacc gtgttgcccc cgaacaaaca gaacaggttg agtcctttgt tctgcagctt
     2881 attgccgagc aggagcgggt ttgggagctt attttaacca atgatggtta ttcatggcat
     2941 tgcgcacttt atgatatatt atttcatctg gttttacgaa atatggaaga ccagcctgaa
     3001 catcagaaac agcgaataac aaaaatattt tatgagcccg atgcaagact ggttgcag
//
LOCUS       DBFIFM010000326         3033 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.328, whole genome shotgun sequence.
ACCESSION   DBFIFM010000326 DBFIFM010000000
VERSION     DBFIFM010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.328"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..444
                     /locus_tag="N7C74_004687"
     CDS             <1..444
                     /locus_tag="N7C74_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HGU2434391.1"
                     /translation="LVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKVVRIRLVNI
                     DAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRMLGQVYAPDGMNVNQFMVRA
                     GAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            490..699
                     /gene="hha"
                     /locus_tag="N7C74_004688"
     CDS             490..699
                     /gene="hha"
                     /locus_tag="N7C74_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HGU2434392.1"
                     /translation="MEKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSTSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            737..1075
                     /locus_tag="N7C74_004689"
     CDS             737..1075
                     /locus_tag="N7C74_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434393.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYDASHLRPERRRRDILLEEVLRQAGIPLLRSHDARKLLQMTGEWLNTTG
                     AAQQSPEHRS"
     gene            1315..1569
                     /locus_tag="N7C74_004690"
     CDS             1315..1569
                     /locus_tag="N7C74_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HGU2434394.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGNPLSDAEKQRLSVARKRASFKEV
                     KVFLEPKYKAMLMQMCHEDGLTQAEVLTALIKSEAQKRCV"
     gene            1805..1879
                     /gene="tap"
                     /locus_tag="N7C74_004691"
     CDS             1805..1879
                     /gene="tap"
                     /locus_tag="N7C74_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HGU2434395.1"
                     /translation="MLGKVQDFFLCSLLLRIVSAGWCD"
     gene            1872..2729
                     /gene="repA"
                     /locus_tag="N7C74_004692"
     CDS             1872..2729
                     /gene="repA"
                     /locus_tag="N7C74_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HGU2434396.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSKGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVEWENRLRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDAGRERQDIVTLVKRQLTREISEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
BASE COUNT          820 a          713 c          774 g          726 t
ORIGIN      
        1 ctggtattat ttatcttttc atggccggta ttgtgtgcag atattcatgg acgggttgtt
       61 cgtgtactgg acggtgatac catcgaggtc atggactcac ggaaggtggt gcggatcagg
      121 ctggtaaata ttgatgcgcc ggaaaagaaa caggattatg gccggtggtc gacagacatg
      181 atgaaatcac tggttgcagg aaaaacggtt accgtcacat attttcagcg agatcgttat
      241 ggtcgtatgc tcgggcaggt ttatgcacca gacggaatga atgttaatca gtttatggtc
      301 cgggccgggg ccgcctgggt atatgagcag tacaacacag atcccgtgct gcctgtcctt
      361 cagaacgaag ccagacaaca aaaacgtggg ctctggtcag atgctgatcc ggttcccccc
      421 tggatatgga gacaccgtaa ataaagcgat ggcaatctta tcattcatct gttctcagcg
      481 ggaataaaaa tggaaaaaac aaaacaggaa tggctgtatc aattacggcg atgttcatca
      541 gtcaatacgc tggaaaaaat tattcataaa aacagagaca gtttatcgac cagtgaacgt
      601 gaatcattta attcagcagc agaccaccgg ttagcagaat taatcaccgg aaagttatat
      661 gacaggatac caaaagaaat atggaaatat gtaaggtaat agttcagcat aattaattac
      721 caggagcaga taaacaatga agctgattat ttttattctc attgttctga ttattgcagc
      781 gttacttatc cggattatcc tcagaagtgt aaatcagcat tctcctttgc tgatgcaatt
      841 gcatgcggca ggtatacgga ccggtgatgc agagcgaata ctgtccggcg gtgaatatga
      901 tgccagccat ttacgacctg aacgcagacg ccgggatatt cttctggaag aggttctgag
      961 gcaggctggt attccgttgc tcagaagcca cgatgccaga aaactgctgc agatgaccgg
     1021 agaatggctg aatacaacag gggctgctca gcagtcaccg gaacatcgta gctgacgcct
     1081 tcgcgttgct cagttgtcca accccggaaa cgggaaaaag caagttttcc ccgctcccgg
     1141 cgtttccata actgaaaacc atactatttc acagtttaaa tcacattaaa cgacagtaat
     1201 ccccgttgat ttgtgcgcca acacagatct tcgtcacaat tctcaagtcg ctgatttcaa
     1261 aaaactgtag tatcctctgc gaaacgatcc ctgtttaagt attgaggagg cgagatgtcg
     1321 cagacagaaa atgcagtgac ttcctcatct ggcgcaaaac gagcatacag aaaggggaat
     1381 ccgctttctg atgcagagaa acaaagatta tcagtggccc gtaaaagagc ttcgttcaag
     1441 gaagtaaaag tatttcttga accaaagtat aaggccatgc tcatgcaaat gtgtcatgaa
     1501 gatggcctga ctcaggctga agttctgacc gcactgataa aaagtgaagc gcaaaaacga
     1561 tgtgtgtgat gatgggctta cattcttgag tgttcagaag attagtgcta gattactgat
     1621 cgtttaagga attttgtggc tggccacgcc gtaaggtggc agggaactgg ttctgatgtg
     1681 gatttacagg agccagaaaa gcaaaaaccc cgataatctt cagaaagttt ggcgactgag
     1741 aagattaccg gggcccactt aaaccgtata gccaacaatt cagctatgcg gggagtatag
     1801 ttatatgctc ggaaaagttc aagacttctt tctgtgctcg ctccttctgc gcattgtaag
     1861 tgcaggatgg tgtgactgat cttcaacaaa cgtattaccg ccaggtaaag aacccgaatc
     1921 cggtgttcac accccgtgaa ggtgccggaa cgctgaagtt ctgcgaaaaa ctgatggaaa
     1981 aggcggtggg cttcacctcc cgttttgatt tcgccattca tgtggcacat gcccgttcga
     2041 agggactgcg tcggcgcatg ccaccggtac tgcgtcgccg ggctattgat gcgctgctgc
     2101 aggggctgtg ttttcactat gacccgctgg ccaaccgtgt ccagtgctcc atcaccacac
     2161 tggccattga gtgcggactg gcgacggagt ctgctgccgg aaaactctcc atcacccgtg
     2221 ccacccgggc cctgacgttc ctgtcagagc tgggactgat tacctaccag acggaatatg
     2281 acccgcttat cgggtgctac attccgaccg acatcacgtt cacaccggct ctgtttgctg
     2341 cccttgatgt gtctgaggat gcagtggcag ctgcgcgccg cagccgtgtt gaatgggaaa
     2401 acaggctgcg caaaaagcag gggctggata ccctgggtat ggatgaactg atagcgaaag
     2461 cctggcgttt tgtgcgtgag cgtttccgca gttaccagac agagcttaag tcacggggaa
     2521 taaagcgtgc ccgtgcgcgt cgtgatgcgg gcagggaacg tcaggatatc gtcaccctgg
     2581 tgaaacggca gctgacgcgt gaaatctcgg aagggcgctt cactgctaat cgtgaggcgg
     2641 taaaacgcga agtggagcgt cgtgtgaagg agcgcatgat tctgtcacgt aaccgcaatt
     2701 acagccggct ggccacagct tccccctgaa agtgacctcc tcagaataat ccggcacgcg
     2761 ccggaggcat tcgcacgcct gaagcccgcc ggtgcacaaa aaatcagcac cacatcccaa
     2821 aaacaacctc atcatctacc ttctggtgca tccggtcccc cctgttttga tacaaaacac
     2881 gtctcacaga cgggcaattt tgtttatcca cattaaactg caagggattt ctctgtaaga
     2941 ttacaaccgt tcatgtcata aagcaccagc cgccaggctt acagggtgca atgtatcttt
     3001 taaacacccg tttatatctc ctttaaacta ctt
//
LOCUS       DBFIFM010000327         3026 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.329, whole genome shotgun sequence.
ACCESSION   DBFIFM010000327 DBFIFM010000000
VERSION     DBFIFM010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.329"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..679
                     /locus_tag="N7C74_004693"
     CDS             <1..679
                     /locus_tag="N7C74_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HGU2434397.1"
                     /translation="LTVKKIALPGKLSYNLEEMLSAINFTIPYSIASVSDSSNTADIF
                     PGTPFPLLFEIEVSSCTGNNTNCNSVRFNFNLNTILQVNLMTCGFEDQSIDTGRFVLS
                     RINNENYQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDG
                     AGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRV
                     KIVVMYN"
     gene            complement(717..1616)
                     /gene="nhaR"
                     /locus_tag="N7C74_004694"
     CDS             complement(717..1616)
                     /gene="nhaR"
                     /locus_tag="N7C74_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HGU2434398.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADV
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1682..2848)
                     /gene="nhaA"
                     /locus_tag="N7C74_004695"
     CDS             complement(1682..2848)
                     /gene="nhaA"
                     /locus_tag="N7C74_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HGU2434399.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT          887 a          754 c          649 g          736 t
ORIGIN      
        1 tctgacggta aaaaaaatag cgcttccagg gaagttatct tataatttag aagaaatgct
       61 atctgcgata aatttcacca ttccatactc tatcgcatct gtatcagata gttccaacac
      121 cgcagatatt tttccaggaa ctccttttcc tttgttgttc gaaattgaag tgagttcatg
      181 tacagggaat aatactaatt gtaatagcgt ccgctttaat tttaatttaa atactatttt
      241 acaggtaaat ttaatgacct gtggttttga agatcaatcc atcgacactg gacgatttgt
      301 actgtctcgt atcaataatg aaaattatca gtttcatcat ataagatttg actgcatcca
      361 gggcgagcaa ggtgaattag tttttgaacc cagtgacgtt gaatattact ttgaaccagt
      421 gacgatacag gagtcaggaa ccagcatatt aaaaaatgag ttagaaaaca gtgaggacgg
      481 tgcgggtcaa ataggctttc aactctccaa tgatggtacg aatgaaatcc agtatggtaa
      541 aagcaattac tactcttttc agcatcccca tgaaggaagt aatcagattc ctctatttat
      601 tcgcccgcgt acatatggaa acaatgtctc aagcgggcaa ataatgagcc gagtcaaaat
      661 cgtggtaatg tacaactaat ctatctcccc caccttaact ttgggggagc tgccgattaa
      721 cgcaccgctg gactaaaaag cgcagaataa tccgtattgc agattcgctg taccgccggg
      781 tgctgaatca tccgctcagc aaaaatagca tggtactctt ccatcacatt ctcgacgcga
      841 ccaatttcta cgacagtttt atcggcataa aagtcatatg cataaagcgt tggggcaacg
      901 aagattgcat tgtgcatcgc accaaaagct ttcatcaaag cggcatcatc aaactcgcca
      961 aggatttcta cgtttaatcc ctgggagtta aaccagttaa gtaatttgcg ccctaacatt
     1021 gaacgtcgcc caggaatcaa aagccgccgt tcttcaagac aagctgggaa cggtttttcc
     1081 ggcggtggat ttgtacacca gaaactcacg ccacattcgc caattctcac ggagaacagg
     1141 ccttcctgct gcgtagaatc tatcggacag tcggaaatga tcatatccag tttatgctgg
     1201 cttaattgct ccagcagcat ttcgtgggtg gattcgaagc agcgaagatg aatcggctcg
     1261 ccttctacta ctgcagcatt cagcacgcta ctgaccagac gtttggaaag tacatcagcc
     1321 acgccaacgt caaacaataa attggattct ttgcgatagt tcacgatatc cagcatttcc
     1381 tggctcaagg taaacatttt atcggcatag cgatagacca gctcccccag ctcgctgggc
     1441 tcgagaccac gtcccttgcg cttaaataat ttgccttgca ggcgctcttc cagcgcccga
     1501 atctgtccgg taatggtttg tggtgttaaa taaagcgcct ccgctgcgcc aaccacagaa
     1561 ccttctttat aaacatgcca gaagtaatac aagtggttgt aattgatatg agacatgctc
     1621 atttctctcc ctgataacaa tgaaaaggga gccgtttatg gctccccggt aaaccgtcct
     1681 gtcaaactga tggacgcaaa cgaacgcgta accagctgta tccaattacc gccgaagaga
     1741 tagaaccgac caggataccg agtttcgccc agttaatcag ttctggatct acgctaccaa
     1801 aggccaggct ggcaataaag atagacatag taaaaccgat accgcacagg atccccaccg
     1861 ccataatttg ctgataagtc gttccctcag gcagatgcgc cagtttcaaa cgcagcgcca
     1921 accagcagaa cagactaatc cccagcggtt tgccaatcag caagccagcg atgatcccca
     1981 atggcagaat ggaggtcaag ccatccagcg tgacgccttg cagtgaaacg ccagcattag
     2041 caaatgcaaa cagtggtaaa atcagatacg ccacccacgg gtgcaacaca tgctccagtc
     2101 gcttcgctgg agaacgccca tgcttctctt tcaaaggaat aaagaagccg acaattaccc
     2161 ccgccagggt tgcgtgaacc cccgatttca acactgcagt ccacaacact acgccaacca
     2221 gaatatagac gcccgtgcgg cgtacaccac acagattcaa taccgcgagt acagcaattg
     2281 ctacagccgc gacgccaaga gaggccatcg ataagtcatt agtgtagaac aatgcgatga
     2341 taatgatggc cccaagatcg tcgataatag ccagagccat caaaaagatc ttcagcgcta
     2401 acggaacacg acttcccaat agcgccagca caccaagtgc aaaggcaatg tcagtagccg
     2461 ccgggatcgc ccacccttcg cgggtaatcg gatcggcata gttaaaggcc agatagagta
     2521 atgccggcac aatcatccca ccaatagcag cgataactgg aaatgcggcc tggcgtaagc
     2581 tggctagcga tccttgcatc agttcacgtt taacttccag accgactaac aggaaaaata
     2641 ccgccatcag cgcgtcattt atccataaca gcatgttttt gttgatttcg agtgaaccaa
     2701 cccggagctg aaccggcgtc tccagaaagt cgtgatacca tccactggtt gcgccgctgt
     2761 tggccatcat catcgccagg atagcggcaa tgataagaat aatgcctccc gaggcatcac
     2821 tgctaaagaa tcgatgcaga tgtttcactt tttatttctc tttcaggtga atagatcgtt
     2881 tttactattt taccccgcac gaatcatcgt taaaatcaga ttatagtgta ggaataattc
     2941 gttttttacg atctatagaa gaatttgcgc catcagcggt gagatgcgtt tttgacggct
     3001 gtaagcgctg acgggtgacc ctaagc
//
LOCUS       DBFIFM010000328         3020 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.330, whole genome shotgun sequence.
ACCESSION   DBFIFM010000328 DBFIFM010000000
VERSION     DBFIFM010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.330"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            87..3015
                     /gene="ehaB"
                     /locus_tag="N7C74_004696"
                     /pseudo
     CDS             87..3015
                     /gene="ehaB"
                     /locus_tag="N7C74_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
BASE COUNT          825 a          696 c          703 g          796 t
ORIGIN      
        1 ataatcgtca acgcgaaaca taagatgttt tctatgcatt gataattgat ggatcaactt
       61 attacgtccc tgaggaggga tgacaaatgc actcctggaa aaagaaactt atagtatcac
      121 aattagcatt ggcttgcact ctggctatca cctctcaggc taatgcagcg accaacgata
      181 tttctggtca aacttacaat actttccatc actacaacga cgccacctat gctgatgacg
      241 tttactatga tggttatgta ggctggaaca actatgccgc tgatagctat tacaacggcg
      301 atatctaccc ggtcattaat aacgctaccg ttaacggcgt gatttctacc tactatctgg
      361 acgacggtat ttctaccaat accaacgcca atagtctgac aatcaaaaac agcactattc
      421 acggtatgat tacctctgag tgcatgacta ctgattgtgc tgatgaccgt gctactggtt
      481 atgtttatga tcgtctgaca ctgagcgttg ataattcaac gatcgatgac aactacgagc
      541 attatactta caacggtacc tataataatg ccgctgacac tcatgttgta gatgtttacg
      601 atatgggtac tgctattaca ctggatcagg aagttgatct gtccatcact aataactctc
      661 atgtagcagg tattacgctg actcagggtt atgagtggga agatattgac gacaacacag
      721 tcagcactgg cgtaaacagc agcgaagtgt ttaataacac tattactgtt aaagattcta
      781 ctgtgacctc tggttcatgg actgatgaag gtactactgg ttggtttggc catactggta
      841 atgccagcaa ctatagcaac acgctgactg cagacgatgt tgcaattgcc gcaatcgcaa
      901 atccgtatgc tgataatgcg atgcagacta cagtaacttt agacaactca acactgatgg
      961 gtgatgttgt tttctccagt aatttcgatg aaaacttctt cccgcaaggt gctaacagct
     1021 atcgcgatgc tgatggtgat gtagatacca acggttggga tggcacagac cgtatggatg
     1081 tgactctgaa caacggcagc aagtgggttg gcgctgcaat gtctgttcat atggttgatg
     1141 aagatggtga tggttcttac gacggatatg ctgttggtac tgaagcaact gcaactctgc
     1201 tcgatattgc agctaacagc ctgtggcctt catcaactgt cggtgttgat aacatcaata
     1261 ctcaatatga cgaaaatggc catatcgtag gaaacgaagt ttaccagagc ggtttgttta
     1321 atgtgacttt gaacggtggt tcagagtggg atacaacaaa atcttctctg attgatactt
     1381 taagtattaa cagcggttcc caagttaatg ttgcagactc tcgtctgatc tctgactggc
     1441 ggttctaacc tgaacatcgg tgaagacggt catgtagcga ctaataccct gaccatcgac
     1501 aatagtaccg ttaaaatgtc tgatgatgtt tctgcgggct ggggtttaga agatgctgca
     1561 ctgtacgcaa ataccatcac cgtaactaac gacggtctgt tggatattaa cgttgatcag
     1621 ttcgatgcta acccgttcca ggccgatacc ctgaatctga ccagtaccac tgatactaac
     1681 ggcaacattc acgctggtgt attcgatatc catagcagtg attacgtaat ggataccgat
     1741 ctggtcaacg atcgtaccaa cgatactacc aagtcaaact acggttatgg cttaatcgca
     1801 atgaactctg atggtcacct gactattaac ggtaacggcg ataacgacaa cactgcttct
     1861 atcgaagctg gtcagaacga agttgataac aacggtgacc atgttgcagc cgcgaccggt
     1921 aactacaaag ttcgtatcga caacgctact ggtgctggtt ctatcgctga ctacaacggc
     1981 aacgagctga tctacgtcaa cgacaaaaac agcaacgcga ccttctctgc tgctaacaaa
     2041 gctgacctgg gtgcatacac ctatcaggct gaacagcgcg gtaacaccgt tgttctgcaa
     2101 cagatggagt tgaccgacta cgctaacatg gcgctgagca tcccatctgc gaacaccaat
     2161 atctggaacc tggaacaaga caccgttggt actcgtttga ccaactctcg tcatggcctg
     2221 gctgataacg gcggcgcatg ggtaagctac ttcggtggta acttcaacgg cgacaacggc
     2281 accatcaact atgatcagga tgttaacggc atcatggtcg gtgttgatac caaaattgac
     2341 ggtaacaacg ctaagtggat cgtcggtgcg gctgcaggct tcgctaaagg tgacatgaat
     2401 gaccgttctg gtcaggtgga tcaagacagc cagactgcct acatctactc ttctgctcac
     2461 ttcgcgaaca acgtctttgt tgatggtagc ttgagctact ctcacttcaa caacgacctg
     2521 tctgcaacca tgagcaacgg tacttacgtt gacggtagca ccaactccga cgcttggggc
     2581 ttcggtttga aagccggtta cgacttcaaa ctgggtgatg ctggttacgt gactccttac
     2641 ggcagcattt ctggtctgtt ccagtctggt gatgactacc agctgagcaa cgacatgaaa
     2701 gttgacggtc agtcttacga cagcatgcgt tatgaactgg gtgtagatgc aggttatacc
     2761 ttcacctaca gcgaagatca ggctctgact ccgtacttca aactggctta cgtctacgac
     2821 gactctaaca acgataacga tgtgaacggt gattccatcg ataacggtac tgaagggtct
     2881 gcggtacgtg ttggtctggg tactcagttc agcttcacca agaacttcag cgcctatacc
     2941 gatgctaact acctcggtgg tggtgacgta gatcaagact ggtccgcgaa cgtgggtgtt
     3001 aaatatacct ggtaatattc
//
LOCUS       DBFIFM010000329         2996 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.331, whole genome shotgun sequence.
ACCESSION   DBFIFM010000329 DBFIFM010000000
VERSION     DBFIFM010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.331"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..528
                     /locus_tag="N7C74_004697"
     CDS             <1..528
                     /locus_tag="N7C74_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2434400.1"
                     /translation="AAIALPFSTMAAPTVTFQGEVTDQTCSVNINGQTNSVVLMPTVA
                     MADFGATLADGQSAGQTPFTVSVSNCQAPTGADQAINTTFLGYDVDASTGVMGNRDTS
                     SDAAKGFGIQLMDSSTSGNPVTLAGATNVPGLTLKVGDTEASYDFGARYFVIDSAAAT
                     AGKITAVAEYTLSYL"
     gene            614..1315
                     /locus_tag="N7C74_004698"
     CDS             614..1315
                     /locus_tag="N7C74_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309306.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU2434401.1"
                     /translation="MNIFAYLLVLVFSMSMSSSAFASVVMTGTRIIFPGDAKEKTIQL
                     RNTSDQPYIINIHVEDERGSDKNVPFMPTPQTFRMEAAAGQALRLLYTGNNLPQDRES
                     VFWFSFSQLPYLNKNDKSQNQLILALTNRVKIFYRPSSIVGKSSDAPKNLTYQVKQNR
                     IEVTNPTGYYVTIRAAELLNNGKKVPLANSVMIAPQSTTEWTLPSGISVAPGAQIHLV
                     TVNDYGVNVTSEHAL"
     gene            1368..>2996
                     /locus_tag="N7C74_004699"
     CDS             1368..>2996
                     /locus_tag="N7C74_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU2434402.1"
                     /translation="MNKSVVSISAAMLVLLCQPVMGSEISPATPSDEDNYTFDPQLFR
                     GSRFSQSSLAKLTTRESVAPGNYKMDIYTNNKLSGSWNVTFKEAADGRVLPCLTPEVA
                     DAIGLKTGEDKGEKDPVCTFAKELAPGITSQTQLSQLRLDLSVPQSQLISRPRGYVPP
                     SELDTGASLAFMNYIANYYNVAYSGQNAHSQRSLWASFNGGINLGAWQYRQLSNMTWD
                     NDKGNQWNNIRSYLQRPLPAINSQLMMGQLITSGRFFSGLSYHGVSLATDERMLPDSM
                     RGYAPTIRGVAATNARVSVMQNGHEIYQTTVAPGPFEINDLYPTSYSGDLDVTVTEAN
                     GAVSRFSVPFSAVPESMRPGTSRYNVEVGKTQDSGDDSMFGDLTWQHGMTNTLTFNSG
                     SRIADGYQALMLGGVYGSSLGAFGANLTWSHARVPESEAQSGWMSQLTWSKTFQPTST
                     TVSLAGYRYSTSGYRDLADVLGERHAASNKQSWDSSQWRQQSRFDLTLSQSLANYGNL
                     FVSGSTQNYRGGKSRDTQLQLGYSNSFSHGISMNL"
BASE COUNT          758 a          768 c          725 g          745 t
ORIGIN      
        1 gcagctattg cattaccttt ttcaactatg gctgcgccta cagtcacttt tcagggtgaa
       61 gtaaccgatc agacctgttc cgtaaatatc aacggtcaaa ccaattcagt agtattgatg
      121 ccgaccgtag ccatggctga cttcggtgca actttagctg atggtcagag cgcaggccag
      181 acgccgttta cggtttctgt gtctaactgc caggctccaa ctggtgcaga tcaggcaatc
      241 aacaccacct tcctgggcta cgacgttgac gctagcacgg gtgttatggg aaaccgtgat
      301 accagcagcg atgcggcgaa aggctttggc attcagttaa tggattccag cacttctggt
      361 aacccagtaa ctctggctgg cgcgactaac gtaccgggtc tgaccctgaa agttggcgat
      421 accgaagcca gctacgactt cggtgcgcgt tacttcgtta tcgatagcgc tgctgccact
      481 gccggtaaaa ttaccgctgt cgcagaatac accctgagct acctctaatt aatctactgg
      541 ctggcagtaa atgccagcca gtatttcttc gcacaattaa tagtgatttt tcatggacat
      601 ggaaaagata agaatgaaca tttttgcata tttactggta cttgtatttt ccatgagcat
      661 gagcagcagc gcgtttgcca gcgtggtaat gaccggaacc cgtattattt tccctggtga
      721 cgcaaaggaa aaaaccatcc agttgcgaaa taccagcgat cagccctata tcattaatat
      781 ccatgttgag gatgaacgtg gttctgacaa gaatgtaccg tttatgccaa ccccgcagac
      841 atttcgcatg gaagctgccg caggtcaggc gttacgcctg ctctacactg gtaataattt
      901 accgcaggat cgcgagtctg ttttctggtt tagtttcagt caactacctt atctgaataa
      961 gaatgataaa agtcagaacc agctcatcct ggccctgact aatcgagtca aaattttcta
     1021 tcgtcccagc tcgattgtcg gtaaatccag tgacgcaccc aaaaacctga cttaccaggt
     1081 aaaacagaac cgcattgaag tgacgaatcc cacgggctat tacgtcacaa ttcgcgccgc
     1141 tgaactgctt aataatggta aaaaagtccc cctcgcgaat tcggtaatga ttgctcctca
     1201 aagcacaact gaatggacac taccctctgg catcagtgtc gctcccggtg cgcagatcca
     1261 tttagtgacc gtcaacgact atggcgtaaa tgttacgtct gagcatgcct tataacctct
     1321 ttctcctttt ttgagcggta tcctttttca cgcacgagta cgtcgagatg aataaatccg
     1381 ttgtgtcaat ttctgcggca atgttggttt tactttgcca accggtcatg gggagcgaaa
     1441 tctcacccgc aacaccgtca gatgaagaca actacacctt tgacccgcaa ctcttccgcg
     1501 gcagcagatt tagtcagtcg tcattagcaa aactgacaac acgtgagtct gttgcaccgg
     1561 gcaattataa aatggatatc tacaccaaca ataagttgtc aggcagttgg aatgtcacgt
     1621 ttaaagaagc cgctgatggt cgcgttctgc cctgcctgac gcctgaagtc gcggacgcga
     1681 taggcctcaa aacaggggaa gataaggggg aaaaagatcc tgtctgtacg tttgctaagg
     1741 aactcgctcc cggcatcacc agccagacac agttgtcaca attgcgcctg gacttatcgg
     1801 tgccacagag tcaattgatt agtcgccctc gcggctatgt tccccccagc gagctggata
     1861 ccggagcatc gctggcgttc atgaattata ttgccaacta ttacaacgtt gcctattcag
     1921 ggcagaatgc tcatagccag cgttcgctat gggcatcatt taatggtggc atcaaccttg
     1981 gtgcctggca atatcgtcag ttatccaaca tgacctggga taatgacaaa gggaatcagt
     2041 ggaacaatat tcgtagctat ttgcaacgcc cgctgcccgc cataaatagc cagttaatga
     2101 tggggcagct tatcaccagc ggaagatttt tctctggact cagttatcac ggcgttagtc
     2161 tcgcgaccga tgaacgtatg ctgccggact ccatgcgcgg ctatgcgccg actattcgcg
     2221 gcgtggccgc aacaaacgcc agagtctcgg taatgcaaaa cggtcatgaa atatatcaga
     2281 ccaccgtggc tcctggccct ttcgagataa acgacctata ccccaccagc tacagcggcg
     2341 atctggatgt caccgttacg gaagctaacg gcgcagtcag tcgtttcagt gtcccctttt
     2401 cagccgtacc agaatcgatg cgtccaggaa cttcccgtta taacgtggaa gtaggtaaaa
     2461 cgcaggatag tggtgatgac tcgatgtttg gtgaccttac ctggcagcac gggatgacta
     2521 atacgctgac atttaacagt ggttcgcgta tcgctgatgg ctaccaggcg ctgatgctgg
     2581 gcggagtcta tggcagttcg ctgggggcat ttggggcaaa cctcacttgg tcccatgcgc
     2641 gtgttcccga aagcgaagcg cagagtggtt ggatgtcgca attaacctgg agtaaaactt
     2701 tccagcctac ttcaaccacc gtctccctgg caggttatcg atactctacc agcggctatc
     2761 gtgatctggc tgatgtgctg ggagagcgtc atgctgccag caataaacag tcatgggact
     2821 ccagccagtg gcgtcaacag tcgcgcttcg atcttacgtt aagtcagagc cttgcgaatt
     2881 acggcaacct gtttgtgtca ggttcaacac agaactaccg tggcggcaag agccgtgata
     2941 cacagcttca gttaggttac agcaatagct ttagccatgg catcagtatg aacctt
//
LOCUS       DBFIFM010000330         2996 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.332, whole genome shotgun sequence.
ACCESSION   DBFIFM010000330 DBFIFM010000000
VERSION     DBFIFM010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.332"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            44..676
                     /gene="comR"
                     /locus_tag="N7C74_004700"
     CDS             44..676
                     /gene="comR"
                     /locus_tag="N7C74_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HGU2434403.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTGKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(738..1277)
                     /locus_tag="N7C74_004701"
     CDS             complement(738..1277)
                     /locus_tag="N7C74_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HGU2434404.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDEHRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1504..2808)
                     /gene="ndh"
                     /locus_tag="N7C74_004702"
     CDS             complement(1504..2808)
                     /gene="ndh"
                     /locus_tag="N7C74_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HGU2434405.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDRDAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVELTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKLLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT          739 a          794 c          726 g          737 t
ORIGIN      
        1 ttataataac ctttcattca cagtcaccag gtacaacggc atcatggcaa ctgactcaac
       61 acaatgtgta aaaaaaagcc gtggccgccc aaaagtgttc gacagggatg ccgcgcttga
      121 taaggccatg aaattgttct ggcaacacgg ttatgaagcg acttctcttg cggacctcgt
      181 cgaagcgacc ggagccaaag cgcccacgct atacgcggaa tttaccaaca aagaggggtt
      241 atttcgtgcc gttctcgacc gctatatcga tcgttttgcc gctaagcatg aagcacagct
      301 gttttgtgaa gagaaaagcg tggagtctgc gctggctgac tattttgctg ccatcgccaa
      361 ctgctttacc ggcaaagaca ccccggcagg ctgcttcatg atcaacaact gcaccaccct
      421 ctccccagat tcaggagata tcgccaatac gttgaaatca cgccatgcga tgcaagagcg
      481 cactttgcag cagtttttat gtcaacgaca agcgcgcggg gaaatcccga cccactgtga
      541 cgtgacacat ctggcagaat tccttaattg tattattcag gggatgtcga tcagcgcacg
      601 cgaaggtgca tcgttggaaa aactgatgca gattgccgga acgactttgc gtttatggcc
      661 cgaactggtg aaataagtcg caaaaaaagc ccctcagcct gagcgaatga ggggccaaat
      721 tgcagagtac agccttgtta tactttgtta ttcaaaatca gttgcccgtt actatctagc
      781 gggatctgcg tacccggatc gcggtccatg cggattttgc cctgctgatc gccaatctta
      841 taggtcacat cataaccgag cattttttct gacttgtcat acaccgtttt acaacgctgt
      901 tgcgtagtcg tgtaagtatc gctttcctgg agagagccct ggatctggtt accggcatat
      961 ccaccaccca gcgcccccac aacagtggcg acatctttac cacgaccacc accaaactga
     1021 tgccctatca cgccgccagc aacagcgccg agcaccgacc cggtaatgcg atgttcatcc
     1081 tgcaccggtc gacgatgggt cactgtgacg ttgcgacact cctgacgcgg tgttttaacc
     1141 gtttccttga ttggggttgc agaaacaacc tgagcgtatt gcgggccacg ttcaaacacg
     1201 ttcagactgg ccactgccgc tacgccaagc gcagctgcga caccaatccc gatacccgcc
     1261 aacattgatt tattcacggg aatacctcct tctttgcagt tatgccaatc atgcaagcga
     1321 aggagaaaag cagctatcag acagaagatg aaaagcgcgg gaatggctgg aaatacttaa
     1381 ggattcggag gagtctgaag gaatgcctga tgcgacgctt aacgcgtctt atcaggccta
     1441 ccgctcgtac catttgtcca gggcggatga ggcgtttatg ccacatccgc cagtgtacgt
     1501 cgattaatgc aacttcaaac gcggacggat aacgcggtta atactcccca ccagcatcat
     1561 taatccggtt ttaaagtaac catgcagcgc aatctgatgc attcggtata gcgagatata
     1621 tacaaagcgc gcaattcgtc cttcaatcat cattgagccg cgcgtcaggt tacccatcag
     1681 gctaccaacg gtggagaagt tcgacagcga taccagcgaa ccatgatctt tatactgata
     1741 atttttcagc agcttaccgt tcatctgcgc cagaatgttg ttcattgcgc aagtcgccat
     1801 ctggtgtgca gcctgagcac gcggcggaac aaagccccct tccggacgcg ggcatgacgc
     1861 gcagtcgcca atagcgtaaa tgtctggatc gcgggtggtt tgcagcgtca gttccaccac
     1921 cagctggttg atacggttag tttcaagacc accgatatct ttcaggaagt ctggcgcttt
     1981 gatcccggct gcccatacca tcagatcagc ctcaatatat tcgccatctt tagtgtgcag
     2041 gccgccttca tcagcactgg tgaccatggt ttgcgtcaga acgcgaacgc caagtttcgt
     2101 tagctcgttg tgggccgcag cagagatacg tggcggtaat gcaggcaaaa tacgttctcc
     2161 cgcttctacc agcgttacgt tcagggcttc gttggtcagg cctttgtaac cgtagctgtg
     2221 cagttgcttg accgcgttgt gcaattcagc ggagagttct acacccgtcg cgccgccgcc
     2281 gacaatcgca atgttcactt tgccatttgc gcccaggttg gcggagtatt tcaggaagag
     2341 attcagcatc tcctggtgaa agcgacgcgc ctggtgcggg ttatcgagga aaatgcagtt
     2401 ctctttgaca cctggtgtat tgaaatcgtt agaggtgcta cccagcgcca ttaccagggt
     2461 gtcataggcg attttacgtt ccggaaccag cagttcacct ttctcatcgc gcagttctgc
     2521 aatagtgatt gttttcgcgt cacgatcaat atcaatgacg gaacccagct ggaactggaa
     2581 accatgattg cgcgcatggg ccagatagct caacgcatcg acgccttcat caagcgagcc
     2641 agtcgctact tcgtgcagca gcggtttcca caggtggctg tggttacgat cgaccagcgt
     2701 aattttggct tttttcttgc gtcccagctt atgccccagc tgtgttgcca tttccagccc
     2761 accagcaccg ccgccgacaa tcacaatttt tttcaatggc gtagtcaacg tgaccccctt
     2821 aaatttatta accaattgtt aattaatggt tattaacata gctcttaatt aacaacaggt
     2881 tacagcaatg aaaatgttct tcgctattga gaataacatg aatggtgcat tggtcatacc
     2941 aaaattgatg ttaatcaagt tttgttgctg aaaagatagg cgactttacc gggtga
//
LOCUS       DBFIFM010000331         2991 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.333, whole genome shotgun sequence.
ACCESSION   DBFIFM010000331 DBFIFM010000000
VERSION     DBFIFM010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.333"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..832
                     /locus_tag="N7C74_004703"
     CDS             <1..832
                     /locus_tag="N7C74_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2434406.1"
                     /translation="IQSDWVEVGPFGNGEKLKVKIESLGKTSDTIGKSSNAQAVLPYV
                     VKIARGTPDFTGERKSTWFISDTVIANIGGESSSSIDFWLGICKALKFNWCVNYLTSK
                     LAGDTFTLGLNISYYPKNTTCKPENTVIKVDDIALFQLRNQGKIAANSKEGTITLKCD
                     NLFGDKKQASRNMVVYLSSSDLVKGSNTILRGKTDNGVGFVLDLTEPPKGTEAAIKIS
                     ANGDQGAATSLWKTDKPGVSLNSNIINIPVMASYYVYDEKKVKSGALEATALINVKYD
                     "
     gene            complement(914..1252)
                     /gene="rcnB"
                     /locus_tag="N7C74_004704"
     CDS             complement(914..1252)
                     /gene="rcnB"
                     /locus_tag="N7C74_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HGU2434407.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHYKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1470..2327)
                     /gene="rcnA"
                     /locus_tag="N7C74_004705"
     CDS             complement(1470..2327)
                     /gene="rcnA"
                     /locus_tag="N7C74_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HGU2434408.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHEHHHHDHEHHQDHDHDHDHDHDHDHEHHHHHEHGDNEEY
                     QDAHARAHANDIKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGA
                     TLVVSFSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMG
                     VHGFMGIMR"
     gene            2448..2720
                     /gene="rcnR"
                     /locus_tag="N7C74_004706"
     CDS             2448..2720
                     /gene="rcnR"
                     /locus_tag="N7C74_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HGU2434409.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            complement(2830..>2991)
                     /locus_tag="N7C74_004707"
     CDS             complement(2830..>2991)
                     /locus_tag="N7C74_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434410.1"
                     /translation="DQQAMLSLITSNKCRVLDESMTVEIQSVDDKGFVSFITPGGHGG
                     WAVKQFFEN"
BASE COUNT          854 a          628 c          717 g          792 t
ORIGIN      
        1 tatccaaagt gattgggttg aagttgggcc ttttggtaat ggcgaaaaat taaaggttaa
       61 aatagagtct ttaggtaaaa ccagcgacac aattgggaaa tccagcaatg cgcaggcagt
      121 attaccttat gtggttaaaa tagccagagg cacacctgat tttactggag aaagaaaatc
      181 tacctggttt atttcagata ccgtgattgc aaatattggc ggtgagtcat cgtcatccat
      241 cgatttttgg ttgggtattt gtaaggcatt gaagtttaac tggtgtgtga attatctcac
      301 cagcaaactg gcgggggata catttacgct tgggttaaat atttcctatt atcctaaaaa
      361 tacgacctgt aagcctgaaa acaccgttat aaaagtagat gatatcgcct tgttccagct
      421 cagaaatcag ggaaagattg cggcgaacag taaggaagga acaattacgt tgaaatgtga
      481 taatcttttc ggcgacaaaa aacaagcatc gcggaatatg gttgtatatc tttctagcag
      541 tgacttagtt aaaggaagta atactatttt gcgtggtaaa acagataatg gtgtagggtt
      601 tgtgttggat ctaacagaac caccaaaagg gactgaggct gccattaaaa tttcggccaa
      661 cggcgatcag ggcgcggcga catcattatg gaaaacagat aaaccaggag tttcattaaa
      721 tagcaacatt attaatatac cagtcatggc cagttactat gtatatgatg aaaaaaaagt
      781 taaatctggc gcactggaag caaccgcatt aatcaacgtg aaatacgatt aataacaagc
      841 aagatgagca agtggctaat aataagaaat aataaggtgt ctgcatttcc ccctctctcc
      901 gagggggctt tttttagcga tgataaaaaa tctcaccgtc gtaggcttta atgattttac
      961 cgtcggtgtc gctgatcaac acgtacgcgc cacccatata ggtccagtgc gtcccggcat
     1021 ccggcgcggg caggttacgc aactgccact gcttaatgtt gtactcatcg gtacggtaca
     1081 tctctggtac ggtgtcccct ggtttgtaat gcttaaagtc agcaatgaaa ctgcttaact
     1141 catacttaga aattcccgag gtattggtcg aacccgcggt ggctggtgcg gcccaggcgg
     1201 cactggtaac cagcaaaagc gcacccagca acatcttatt tttaatggtc ataaactctc
     1261 cactttgggg atctgtaata tcagtcgcat atctttcctg ccatacaata taactgacaa
     1321 gcgatatttt gttagtgatc ccatgatttc gtaaaaagtg taaatgaaag gatgtagcaa
     1381 aagcatcctc attgagcgag aagtgagcgg aataagctga tttaagaatc atcctgggct
     1441 aaaaggatag cgaagcccag gtgattgttt tatctcataa tacccataaa gccatgcact
     1501 cccatataca ccccgactaa accaattaac agactggaaa aatagggggc gcgtttagcg
     1561 agagtgttaa atccgctcca gcgttttgca acttgttgaa cactgattgc cgctccaacg
     1621 cctactgtaa ccagcgttag tgccagaccg atgctgaaac tgacgaccag tgttgcgccc
     1681 agtgtcaggg ctttcaactg aatgcaaatc aacagcacgg taattgctgc cgggcaggga
     1741 ataaggccac ctgttaagcc aaataacaaa atttgccagt tggtgacctc tctaccatca
     1801 aagcgtcgtt taatgtcatt ggcatgggct cgtgcatggg catcctgata ctcttcgttg
     1861 tcgccatgtt catgatggtg atgatgttcg tggtcgtggt cgtggtcgtg gtcgtggtcg
     1921 tggtcgtggt cttgatgatg ttcgtgatcg tggtgatgat gctcatgcat attctccagc
     1981 cagttgcgtt cgccgcgcca ggtacgccag aacatccaga aagcggtgct aataatgatc
     2041 actgcggaaa tcaactggag ccacggttct gctgattgag cagtaaagcg cttgctgatc
     2101 accatcccgc caaaggcaat caaccagacc actgcggtat gcgaaatggt tgctgccagt
     2161 ccgagcatca ccgcctgttt aatggtgcct ttgatggcga tgataaacgc cgccatcatc
     2221 gtttttgagt gccctggttc caggccatga agcgcaccaa gtaagatggc gctggggatg
     2281 aagaaccagg cgtttccttg ctgaagaaga gttgtaaatt cggtcatgat aataattctt
     2341 agtattaatt cggcaatctg attctactcc cccccagtac ctgatactac cccccagtag
     2401 attaatagtg ctatgatttt tcatgttctt gttaaccagg tgttgccatg tctcatacaa
     2461 tccgtgataa acagaaactg aaagcgcgtg ccagtaagat tcagggccag gtcgtggcgc
     2521 tcaagaaaat gctcgacgag ccgcacgaat gcgctgcagt tttacaacag attgctgcta
     2581 tccgtggcgc ggtaaacggt ctgatgcggg aagtgattaa aggtcatcta acggaacata
     2641 tcgttcacca gggggatgag ctaaaacgtg aagaagatct ggatgttgtt ctgaaggtgc
     2701 tggattctta tatcaaatga aagtgcctaa tatggccgta gttgaggcaa taaatagagg
     2761 gacaagtgac gttaagctga attagagtaa atggcgtgag gacacacgcc attcgaggtt
     2821 atagtttgat taattttcga aaaattgctt cacagcccag ccaccatgac cacctggagt
     2881 tatgaatgaa acaaaacctt tgtcatctac gctctggatt tctaccgtca ttgattcatc
     2941 taacacgcga catttattgg atgtaatgag actcagcata gcctgttggt c
//
LOCUS       DBFIFM010000332         2966 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.334, whole genome shotgun sequence.
ACCESSION   DBFIFM010000332 DBFIFM010000000
VERSION     DBFIFM010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.334"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..493
                     /locus_tag="N7C74_004708"
     CDS             <1..493
                     /locus_tag="N7C74_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU2434411.1"
                     /translation="SLGLRAEKIRSVYRESDIIPGEQTATSILKQRTKNIALPSHTHQ
                     QQNPPQEKTVVSIAVDPESPESFRKRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHH
                     LIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATG
                     VLG"
     gene            506..739
                     /locus_tag="N7C74_004709"
                     /pseudo
     CDS             506..739
                     /locus_tag="N7C74_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
     gene            735..2036
                     /locus_tag="N7C74_004710"
                     /pseudo
     CDS             735..2036
                     /locus_tag="N7C74_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
     gene            2184..2366
                     /locus_tag="N7C74_004711"
     CDS             2184..2366
                     /locus_tag="N7C74_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU2434412.1"
                     /translation="MRFVQLILLYFCTVVCTLYLVSGGYKVIRNYIRKKIDAAAAEKI
                     SASQSAGTKPEEPLIS"
     gene            2404..2673
                     /locus_tag="N7C74_004712"
     CDS             2404..2673
                     /locus_tag="N7C74_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU2434413.1"
                     /translation="MSEIKSLVTAEAVKDVLRSEEVRSALKQQLRQNLEARLDAEVDA
                     ILDELLGGPAAPEPEDGAGDSAVSDGVVSQPDGSSEPQPGGEMMM"
     gene            2749..2964
                     /locus_tag="N7C74_004713"
     CDS             2749..2964
                     /locus_tag="N7C74_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU2434414.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
BASE COUNT          759 a          692 c          869 g          646 t
ORIGIN      
        1 ttcgctgggg ttgcgtgcgg aaaaaatccg ctccgtatac cgtgaaagcg acatcatacc
       61 gggagaacag accgccacca gcatactgaa gcagcgcaca aaaaatattg cgctaccgtc
      121 tcacacccac cagcaacaga acccaccaca ggaaaagacg gtggtcagca ttgccgttga
      181 tccggagtct ccggaatcct tcaggaaacg acctaaacgt cgccgctggg taaatgagaa
      241 atacacacgc tgggtaaaga cacagccgtg tgcgtgttgt ggtaagccag cggacgatcc
      301 tcatcatctg attggtcatg gtcagggcgg aatgggaaca aaatcccacg atattttcac
      361 gctaccgctg tgtcgggagc atcacaacga gcttcatgcg gatccgctgg cgttcgaaga
      421 aaagcatggt tcccaggttg atttaatttt tcgttttctt gatcacgcct ttgcaaccgg
      481 cgtgctcggg taaaagaggt tactgatgcg tatagagttt gttttgcttt acccgccgac
      541 ggtgaacacc tactggcgac gtcgtggcag cacatatttt gtatcaaaag ccggtgagcg
      601 ttatcgccgg gctgtggcgc ttattgttcg ccagcagcgg ctgaaattaa gcctgtccgg
      661 aaggctggcg ataaagatta ttgccgagcc accggataag cgccgccgtg acctggacaa
      721 tattctgaaa gcgccgctgg ggtgtgggca ggccgctgta taaggatctc atcggtcgta
      781 caaaagccgc gctggcaaag aacccgaaaa atgtgctgct tgccgtggtg tggatgcagg
      841 gggaatttga ctttgacgga acgccagcaa atcacacagc ccgttttaca gaagtagtgg
      901 aacaatatcg tacggacctt gcagatatgg tgggacagtg cgctggtggt tctgctgacg
      961 gtgttccctg gatatgtgga gacacaactt atttctggaa gcagaagagc gaatccactt
     1021 accagacggt gtacggcagt tacaaaaaca aaacggaaaa gaatattcac tttgtgccgt
     1081 tcatgaccga tgagaacgga gcaaatgtcc cgacgaacaa accggaagaa gacccggata
     1141 ttccggcatc aggatattac ggtgcggcct cccggacgtc ggcaaactgg acgtcagcag
     1201 accgtgcgag ccatttcagc tcatgggcac gcagggggat tatttctgac cgtcttgcct
     1261 cagcgattct tctccatgca ggacggacgg ctgaactggt gggtggggaa caggttgtga
     1321 tgccgccgga tgagaagccg tcaccggaca caccatcaac accgtcaacg gacgggaaat
     1381 cagtgacaac gctgctttat taccgtgcaa cagagtcagg tggtttactg aatccgcagg
     1441 gatggggagc tgaaggaggg cgtgcattgg tagttgatga tgcaggtgct gcaggaggta
     1501 aggcgctgag gtggaccaaa cagacaggaa gttcctcgtg gtttatgcag catgatgccg
     1561 gtaatggcgc agacctgctg gagaagggcg ggcttatcag ttgtcgtttt aaagttgatg
     1621 gcacactgac agctaatcag tacgcactgg cgctgtactg gccggtttct tcactgcctc
     1681 agggtgtcac actggaaggt aatgccggtc ataacctgct ggcgtcgttt tacgtacaga
     1741 gcgatgccac agaccttaat gtgatgtacc acaagggaaa tgctggtcag aacacgaagc
     1801 tggggtcatt cggcgcattt gataacgaat ggcatacgct gggcttccgt tttgccggta
     1861 acaacagtat tgaggtgacg ccggtcattg atggtaagga cgggacgccg ttcatgctgt
     1921 cacagtcacc ggtcggcacg tttacggcag acaaattgcg cgtgaccgat atcactagcg
     1981 gtgcgacata tccggtgctg attgaaagta taacagtgga agtgaataac ccgtaagcag
     2041 gaaaaaaagg ccgccggggc agggaaaaca aggagccaga accggcggca aatgtcgtta
     2101 tatccaaagc aaaacatgca ggacactttt ttaaccaaca ggtattaacg atgtcaacac
     2161 catatcaata accgggaggg ataatgagat ttgtacagct tattttattg tatttctgca
     2221 cggtggtgtg cacgttatat ctggtaagtg gcgggtataa ggttatccgg aactatatac
     2281 gcaaaaagat tgatgccgcg gcggcggaaa aaatcagcgc cagccagtca gccggaacaa
     2341 aacccgaaga gcctctcatt tcgtagcaac tttcttaaca acacctttca acgagaaaat
     2401 cccatgtcag aaataaaatc tctggtcact gctgaagcag tgaaggacgt cctgcgctct
     2461 gaagaagtca gaagcgcact gaaacagcaa ctccgccaga atcttgaggc gcgtcttgat
     2521 gctgaagtgg atgccattct ggatgagctg ctggggggac cggctgctcc tgagcctgaa
     2581 gacggcgcgg gtgacagtgc tgtttcagat ggcgttgtgt ctcagcctga cggtagcagt
     2641 gagcctcagc ctggcggcga aatgatgatg taaccatacg caggggctgt cggtgtgagc
     2701 tgatgcccct cctgttgttg tgagcttccg gattgcggga gacggggtat gtaccagatg
     2761 gaaaaaatca caacgggtgt gtcatacacc acgtcagcgg tggggacggg atactggtta
     2821 ctgcagctgc tggacaaagt ctctccgtcc cagtgggtgg cgataggtgt gctggggagt
     2881 ctgctgtttg gcctgctgac gtatctgact aacctgtatt tcaaaatcag agaggaccgt
     2941 cgtaaggcgg cgcggggaga gtaaag
//
LOCUS       DBFIFM010000333         2946 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.335, whole genome shotgun sequence.
ACCESSION   DBFIFM010000333 DBFIFM010000000
VERSION     DBFIFM010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.335"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            281..472
                     /locus_tag="N7C74_004714"
     CDS             281..472
                     /locus_tag="N7C74_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU2434415.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMQDGYPHPCM
                     RPMYNFLGKFKPEPREETE"
     gene            complement(525..758)
                     /locus_tag="N7C74_004715"
     CDS             complement(525..758)
                     /locus_tag="N7C74_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434416.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(854..1477)
                     /locus_tag="N7C74_004716"
     CDS             complement(854..1477)
                     /locus_tag="N7C74_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU2434417.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFRFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(1566..2075)
                     /locus_tag="N7C74_004717"
     CDS             complement(1566..2075)
                     /locus_tag="N7C74_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434418.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
     gene            2533..>2946
                     /locus_tag="N7C74_004718"
     CDS             2533..>2946
                     /locus_tag="N7C74_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434419.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVR
                     GKETETISERLIREADTREYELWRPVRRFFSIVFLI"
BASE COUNT          871 a          708 c          603 g          764 t
ORIGIN      
        1 aaattattcc actgaatgct tttaatacag ttggttagct taaaaaatag caatttttga
       61 tttcagtcat tgaaagttgc aatgtttgac gattcaacag catttaagtg agtttcagga
      121 tatgggaagg acatgaaagg aggagcgcaa taaaatacac aggaagaagg ggaaaatgtt
      181 tcgtaaaaac agaaaattaa cagcgcacat caggaaacgc agtctcctga ttcagtgcta
      241 ccacatgaaa atttagttaa ctgttaccgt actaaaattt atgtcagaag atgttccatt
      301 accgaaagtc aatcagcgat acaaagacga tcatggtgcg cttgtcaccc tgaccagtgt
      361 tgaggaaacc cgggtggtgt ttatgcagga cggctatccg catccctgca tgcgaccgat
      421 gtacaacttt ctgggaaaat tcaaacccga accccgggaa gaaacagaat aaaacgaaat
      481 ccctgccggc aatcacacta cctgatagca gcataatcca cttattaatt ctcatccccg
      541 gagacaagcc cagaaaacca ctccgggtgt tcactgtatg ccgtgttcag cgcatccctg
      601 gaaacgttta tataaaagct gtcctgcaat ttgcgggtaa caacttctgc caggcgattc
      661 cggttatcca ccagtccggc ataacctctc tctgccagcc actcattcag ttcatgttcc
      721 agagacagat tgataatatc ataatcggac cttgccattc ccgtttcctt agcacatgtt
      781 ttcaggcacg gggataaagt ataaagaatg tcatactgct gctgccagag caatattccc
      841 cctgatggtt gtatcagaga tggtatgccg gtactttgcg ggttgttccc gtcaggttat
      901 cgtgcacctc cacggtcgtg gtcaccaccg gcattccggc ctttctcagc ctcagaacat
      961 cagccgcaat acgctgactg ccaaacctga acaggccgtc cagtgcagtc accagtaaac
     1021 ccgcctccag tgcatacttc agccgttcac ggggcgcttt cacttcccgg gcaacctgct
     1081 gatatggcaa tgatgtgttt tcattcccaa ccacgcgacg aatacggtga gacagatgat
     1141 accggtatgt atccggcaca ccagaaaggc tggccttcag gctgtacacg cagccaaacc
     1201 gtttatcgtt aaacaccaca tttttctggc tgatgcccca ttcttcacgc agcgcggcaa
     1261 tcaattgcgg tgtacgggta agcagcaagc gaaaaggcag ttcaaaactg gtgacgtaat
     1321 ccacattcag cagtgcaata cgcaaccgtt cttctggccc ggcctctgtc tgccggcact
     1381 cctccagaac atcctgccac tgccggcgaa gacgggggga ttcgttccgt tctgtaaaac
     1441 agtatttatc cgccagatag tcaattcgtg tacgcatact gaaaggaatt ccgtataaag
     1501 attcagcaga caaaacttta tcagccagta aaaattaacg gaagcattga tattcctccc
     1561 gacaatcacc ggatgattgt tgcaataacc tgcgacataa aggactgaac agccgttttt
     1621 aacgccacgt attgccctga tgaaccaggc cgggccaccc gaaaacatct ttccacccgg
     1681 cattgtccgc cagcacttat caccgaccag gtctgttgca gtaaatccac cagttgcgca
     1741 cattcttcat cagccatgac gccgactcgc ggatggcaaa tactcgtctg gctgagcagc
     1801 aacagacatc gtatctgtgc tccttcatgt tgttcccgca accagcgtaa taccaccggc
     1861 gctgatgggc tgaccgtgtg attatgctcc gtaatacgtt cgtgtacccg tcggtgaaag
     1921 gaactacaaa atgtctgaat ctgttgacca tagattcctt ctgccgaata gagcgtgaag
     1981 tgaaatgcca acagatttgg ccagtggtcc accgcctgct gaacaaaacg ccggatttcc
     2041 cccggctctg aaattaaggc ttcggttatt tgcacgaaat gttccttttc aacccctgtt
     2101 gaattttgtc aagccggtgc agacacagta aagcgcgcat caacacctga aaggtacgat
     2161 acaaacacaa gttacaaaca ataagaacgg aaggacaatt aatcagtgag gaataataac
     2221 taaattaacc aaaagaatgt tactgacaat actaccaata cttaaatagc aatatattaa
     2281 ttaatgtaag aacagatgcc cggtaggaca tatacacaat acatccacaa acacactctt
     2341 ttcattaaat caatcacctg attaaaccat gaaagtgtga tgtaagtcaa aattactgat
     2401 actatcaatt gataatgatt atctctatca ttatcgtgaa attcgagaat gaaaggagaa
     2461 gtagtcatta taagcctttt ataaatcgtc atcatcatag taaagtagaa cactttttat
     2521 atgagttact atatgaaaat atactggaca agaaaacgta ttcctgaact ttcagcactt
     2581 ccaccttcat taagaaagaa aaattttact gacgcatata atgcagcatc ttctcatatc
     2641 gaatactgga taggtgctgg cgtctcattt atctcgatga tgattttatc cagagtatat
     2701 gactttcttt tacccgccca agatactttc ccgggtgata tcatcagaag tttatgtgtt
     2761 gtgtgtccat ccatattaat ctggtttcag ttttcggtat atgtcatgag gaaatattac
     2821 agacatattc tggtgcgagg aaaagaaacc gaaaccattt cagaaaggtt gatacgtgag
     2881 gctgatacac gagaatatga actatggcgt ccggtccgtc gctttttctc cattgttttc
     2941 ctcatt
//
LOCUS       DBFIFM010000334         2910 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.336, whole genome shotgun sequence.
ACCESSION   DBFIFM010000334 DBFIFM010000000
VERSION     DBFIFM010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.336"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(169..1647)
                     /locus_tag="N7C74_004719"
     CDS             complement(169..1647)
                     /locus_tag="N7C74_004719"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HGU2434420.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMEKFNIPRHMLFDVQMPGTVLGQITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKALTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
     gene            complement(1674..2909)
                     /locus_tag="N7C74_004720"
     CDS             complement(1674..2909)
                     /locus_tag="N7C74_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HGU2434421.1"
                     /translation="MSFSGGVSFDLAYLRYIYQIPMAKFMGFSNTEIGLIMSTFGIAA
                     IILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVMLCIQVAFAITTILML
                     WSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFSRFAPDDSTSLKTVI
                     IIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVLRISTTWYCSMVIFG
                     VFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPLGGIITTYSKVKSPT
                     RVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYASRGLYWACPGEARTP
                     SYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTQPAAEAYRNMWLMGMAALAMVIVFTF
                     LLFQKIRTADSAPAMASSK"
BASE COUNT          760 a          773 c          665 g          712 t
ORIGIN      
        1 cagggataaa ccgaactagt taaagttttt attatgttcc ctgcatcagc ggggataaac
       61 cggacttgtt atcatttttt aagttgtcga acttatgttc cccgcgccag cggggataac
      121 cattcaggta tgttcaggcc gggcagtttc ccgcccggtc ttcctcactt acgcattcga
      181 ccagctctgg atcgaatccg cattccccaa ttccccatgc atcacttcat acgatttttt
      241 caggatcaca tcagtatgtt tggttagggc tttgaaaata cctttattca tcgcgtcgta
      301 gcgtttggca ttgctctcaa tcggtataaa gatatctttc acgcgaacca ttttgtcgac
      361 agccgttgcg taatccgggt atagccccag acctaccgcc gtattgatcg ctgcccccag
      421 acttgcacaa ccgttaatgg cgttacgtcg tgccggaagg ttgaacacgt cggcaaaaat
      481 ctgcataaac agatcgctgt tcgaaccgcc accagtaatg atcacatgct tcgcaaagtg
      541 attcatttca ttacacatat tgtcgtaatt gttcttcagc gtcagcgcca cgctttccag
      601 tatcgaacga tatatccatg cgtaatccat gctggaatca aagccgatca taatcccgcg
      661 tttgtacggt tcccacggat tggtcagcca gtccagcacc gtcatcagcc cattacagcc
      721 aggtggcaca caagaagctt ttttgttgag taaatcttcc ggtgaaagat cctgcgccct
      781 ggcatcctga attaacgact cgcctaacat gtcgcgcagc cagctcaccg tccacatgcc
      841 tttgcgaata ccgtaacctt catacagcaa tgtttgcgga atagaagaca taatcggcca
      901 gtacgccacc ggatctttcg gcaaggcttt gccgttcatc atcagcgcga tataagtacc
      961 gagtgaaatc accgccgttt catcatccag taatccggct cccagagctt ctaccggttt
     1021 atcactggtg gtacaaacaa ccggcagccc tgccgggaaa tgtgtcgcca gcgcggcttg
     1081 tggtgtgatc tgtccaagaa cagtgcctgg catttgcaca tcaaacagca tatgacgggg
     1141 gatattaaac ttctccatta ccgcagcatc ttcgctccat gcccagctct tataatccac
     1201 cggccactga ccaaaatagt tggcgatatt gtctttaaac tcgccggtta agcgatgcgt
     1261 cagataaccc gaaaaagagg tgacataggc cacgtcagga ttagtgtgtt cgtagggacg
     1321 cgttacgcgt gcatcctgcc agctaatcaa cggcgcagca ggcgtgccat cggctttcaa
     1381 taacgcacgg cagcaacgga tggatcccag accaataccg acaatatctt ctttattccc
     1441 ggcaaactga ctcatcaaat cgtgaccggc aaaacataat gatgcccata aatcgtcgtc
     1501 aggatgttct gcggtatcgg catctggcgt gtgcatcggc tgcaataaac ctttgccttc
     1561 gcaaaccacg ttaccttcca gatcgtacat caccactttt gtgctctgac ttccgccatc
     1621 aatccctatg atgtatttct tcgacatgat ttttctcctg taattttcct ggcttacttg
     1681 ctgctggcca ttgcgggggc gctatcagca gtacgaattt tttggaacag caaaaaggta
     1741 aagacaatca ccatggcaag cgccgccatg cccatcagcc acatattgcg gtaggcttct
     1801 gctgcgggct gggtgtcttg ccagtggccg ataattgggt aaacgaagac atccggcagg
     1861 aatccaatca ccgagcaaat acctaccgtg gtgcccataa tgtaagacgg tgttctcgct
     1921 tcgccagggc aggcccagta cagcccgcgt gaggcgtaac aggtgaatcc cagcagtaaa
     1981 atcaggccaa tccccatcgc gaccgattgc gggttagagt tcgtgacgag cagggcagtt
     2041 aacgccagca ggccgagtac ggaaaggatt tggatcacgc gggtagggga tttcacttta
     2101 ctgtaggtgg tgataatgcc gccaagtggg ccgcacagcg cgcggaagat tttgttgatc
     2161 acaatcccca tgtagctcgc cgccaccagc gacatgccat acatttcggt cagatagttg
     2221 gtggagtaac tcagaatggc gtagatggtg aagacgccaa aaatcaccat gctgcaatac
     2281 caggtggtgc tgatacgcaa aacggcaagg atgtcgctaa gctggaatga ctgtttttct
     2341 tcgttattgg cactgcgcag gttgttgtta tcgctaacaa aaaaccagca cagaatcccc
     2401 aacaagatgt aaaccacact atagatgata atgaccgttt tcaggctggt gctgtcatcc
     2461 ggtgcaaagc gagaaaaaac ccacatggta aacactgcca gcgacattac accgacgccg
     2521 cgcagccctt ccatccagcc cataattttc ccttgctcgc tatgatcgcc aagcaacgat
     2581 gcggctttaa tcgacaccga ccacagcatt aaaatcgttg ttatcgcaaa ggcgacctga
     2641 atacagagca ttacccacag cggtggatac gttgccatta acagacccag taatccggta
     2701 atgatcatcg cggaagtaat cattttgcgg tgtgaaaatt tatcggcaat aacgccgctg
     2761 ggggcataaa gaataatagc cgcaatacca aaggtactca ttattaaacc tatctcggta
     2821 ttgctgaatc ccataaattt cgccatggga atttgataaa tataacgtaa ataagccagg
     2881 tcgaaactaa cgccgccgct aaaactaatt
//
LOCUS       DBFIFM010000335         2837 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.337, whole genome shotgun sequence.
ACCESSION   DBFIFM010000335 DBFIFM010000000
VERSION     DBFIFM010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.337"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(635..832)
                     /locus_tag="N7C74_004721"
     CDS             complement(635..832)
                     /locus_tag="N7C74_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2434422.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(898..1168)
                     /locus_tag="N7C74_004722"
                     /pseudo
     CDS             complement(898..1168)
                     /locus_tag="N7C74_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1473..1661
                     /locus_tag="N7C74_004723"
     CDS             1473..1661
                     /locus_tag="N7C74_004723"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434423.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            1989..2183
                     /locus_tag="N7C74_004724"
     CDS             1989..2183
                     /locus_tag="N7C74_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434424.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            2526..2654
                     /locus_tag="N7C74_004725"
     CDS             2526..2654
                     /locus_tag="N7C74_004725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434425.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
BASE COUNT          849 a          653 c          643 g          692 t
ORIGIN      
        1 caagtatatt atcttctggt agagattcag gatacagttt gtaggagctt tcttttttga
       61 acaatgattt gcaggacagt tatgctaaac tagcggaatc agtatcacaa caataaatga
      121 ctgatcatac ctgaaatttg aattaacagg catcatgaat gtcaatagtc taagcggtga
      181 ctacttatat cgcttgcata aatactaaac actgactaga aaagtattgg ataaccccgt
      241 cactaaaagt aacgcttcgg ttatttgctc ggaatgtatt aacgccaaga ttgttagccg
      301 ttacgcctta tatcactggc gctgacaaat gtttcttttc taatttactg aaccggcgca
      361 gacacgcagg aaagcacgcc ggcaccggtg taatgccagt tctaatcaat aagaatggaa
      421 tgacgaatca gtcaggaagg gataacaacc cgctgctgaa cggaaggtat cagcaggaag
      481 atgaacggga atatcgccag caccaagggc agggccgggt tccacaatac cagcgctggc
      541 attttgcaca ttgccagcgt accggcatta caacacgttt catgctgctg ccccccagga
      601 ttcctcgatt cgttgctaca tccactgctc tacttcactt ccactgctcc acttcacttt
      661 tgtaccagcg gctgctgcgc cccagtttga tgggtttagg gaaatggcct tcactgatca
      721 acttgtaaaa ccatttgtcg gtcataccgg tgaaactggt gatatagttc atgtcgatca
      781 gggaattatc ggcacgcaga aatacgctgt cgtggcttgt tgtagtcaac atggtcagaa
      841 tgtcctgtga gatttatgaa gattgtgcga atgcgggaaa tctactgagc tgtgctttca
      901 gaactggcct gttacgggat accagggatt accggcgggg taacgggctt ccggatcata
      961 cacaccacga ttatcgcgga caaaatcact gaacgcccat atcacctctt taagtatgtc
     1021 ttcgcagtcc ggtaaatgac gatccagcgc cacatcccgg gtggtacagc tttgatgcgc
     1081 ccggtgacac aaagcccgga ttgttccaga catcctgaat caaacgcccc agattagggg
     1141 cgtcgaaata tgcctctctg accattatat tccggcgtat aggtagcagg tctgaagtga
     1201 caatgcgtca cctgacgtta aaagtcaccc aagatgaccc cagtgggggc aacaagtata
     1261 aaaccgtttt ttgattaagt ggtcggatat gaaaagggga aggtttatgt gaaaaatacc
     1321 tgtgttttct ttgaaaacac acaacagtat caggttattt atgtttatac agtgcgttcc
     1381 aaactgacct gaataattac gcaagttgca acaataaaac atttttgccg tagtgtgagg
     1441 tactccggta aattgcttcc caggtgcaga caatgactaa cgaagaacta caggcaatcg
     1501 cattatccat gcttgagcgc cagcgcgaaa aagtacacca gcaacgagag tctttgctta
     1561 acccatctcc attcaccagg ccaaaacgat tggagcttga agcattcttg gacaaatacc
     1621 cacggcggct taaatcaggc aaaaccagac caccaggctg atagctcaca gtttgttctc
     1681 cgattcaata aaacggataa gaaccatcag cgcggtagcg taaggcacta cagcgcattg
     1741 ctttacgagt aataacaatg gatttatcac cagaagacgc aaagttttta cgagaactgc
     1801 aacaccgtat gctcagagaa gccattatcg aacagcaaac gggcatgagc gccatagacc
     1861 cattgcgata caaacgggcc tcatctcctt tagttgacga caattccaat gcggataaaa
     1921 cacgtccacc acactgatta catttttcat tagcacagtg gtgtgatgca gcatacgagg
     1981 aaaaccggat gaatgagaac aaaataaaaa ggctggagca actgttacag gcacgccaac
     2041 gggagtttgc cactaaatgg ggacgtgccg cgctgccaca tgaacgccta acctattttc
     2101 cacttgaaga cctcattaaa aataatggca aaaaaatcag gtctgataag aaacacttct
     2161 tagaatgcaa tgaagacaaa taacaacaat ctgcatattg ccaccaacca cagatgtggc
     2221 atcgttatca gcgcacttga ttttctttat atccactgac gagtttgccg gtcattctgg
     2281 tatcatgcag atatttacgg cgtacaagca cactagcatg acattgtaat aaggcctgag
     2341 cctaacgctt taatgtgtgt catacactca gtggcgtaat cgttgacaat atggtgcatg
     2401 taatcgtaag cgtccagcga aatccatctg gtcataatct gaagcatccg acaaagtggt
     2461 gtccaccaaa taagtgatct gcgcccaata tcctgagccg gaactttgac aaaggccaac
     2521 gaaaaatggg agcggatgtt acagagttct cggtacaggg gaaaagtggt acctgtcgcc
     2581 agttctccga tctgtttaac cgggaaatta tctcctacag cttgtcagaa aggtcggtga
     2641 tggagcatcg ttaatacgat gctatacgat gcattcacag tgcccggcca gaggatgccc
     2701 cgtcgctgca tatggatcag ggttggcaat atcgaattgc aggctatagg caaagttaaa
     2761 gccccatgga gtagcacaaa atatgccgca caaaggaaac ggtctgaata acgcagtgat
     2821 gaagaacttc ttcagca
//
LOCUS       DBFIFM010000336         2818 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.338, whole genome shotgun sequence.
ACCESSION   DBFIFM010000336 DBFIFM010000000
VERSION     DBFIFM010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.338"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..277
                     /locus_tag="N7C74_004726"
     CDS             <1..277
                     /locus_tag="N7C74_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial assembly protein"
                     /protein_id="HGU2434426.1"
                     /translation="LFYRPAGIAPVNKTTFKKLRVSRSGNNVVIKNDSPNWVTISDVK
                     ANNVKVNYETIMIAPQESQNVSEKNNNANGWQLTIIDDHGNYISDKI"
     gene            293..2773
                     /locus_tag="N7C74_004727"
     CDS             293..2773
                     /locus_tag="N7C74_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HGU2434427.1"
                     /translation="MLRMTPLASAIVALLLGIEAHAAEETFDTHFMMGGMKGEQVTNL
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPHETCLTREIVKRLGINSDNFAREN
                     QCLTFEQLVQGGSYSWDIGIFRLDLAVPQAWVEELENGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTDNNPGEWKSNTLYLEHGF
                     SQILGTLRIGDMYTSADIFDSVRFTGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFSISDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFLSQTSTRFGLAAWRYSSRDYRT
                     FNDYVWANNKDNYRRDKNDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYDENHAEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGNRDQFNYGINLSHQHQGNETTAGANL
                     TWTAPAATVNGSYSQSSTYRQVGASVSGGLVAWSGGVNLANRLSETFAVMHAPGIKDA
                     YVNGQKYRTTNCNGVVVYDGLTPYRENHLMMDVSQSDSETELRGNRKMTAPYRGAVVL
                     VDFDTDQRKPWFIKALRSDGQPLTFGYEVNDMHGHNIGVVGQGSQIFIRTNEIPPAVN
                     VAIDKQQGLSCTITFGKEIDESKNYICR"
BASE COUNT          815 a          526 c          703 g          774 t
ORIGIN      
        1 gttgttttac cgacctgctg gtattgctcc ggtaaataaa acgactttta aaaaattacg
       61 agtgagtcgt agcggtaata acgtagtcat aaaaaatgac tcgccaaatt gggtaacgat
      121 ttctgatgtt aaggctaata acgtcaaagt gaattatgaa acaatcatga ttgcaccaca
      181 agaaagtcag aatgttagtg aaaaaaataa taatgcgaat ggctggcaac tgaccattat
      241 cgatgatcac ggcaactata ttagtgacaa aatttaattg caggagctgc ctatgttgag
      301 aatgaccccg cttgcatcag caatagtcgc gttattgctc ggcattgaag cacatgcagc
      361 agaagaaacc tttgacaccc attttatgat gggaggaatg aaaggtgagc aagtaacaaa
      421 tttgcgtctt gatgataatc agcctttacc tgggcagtac gacattgata tttatgtcaa
      481 taaacaatgg cgtggaaaat atgagattat cgtcaaagat aacccacatg aaacttgcct
      541 gacgcgtgaa attgtaaagc ggttaggtat aaatagcgat aattttgcac gcgaaaatca
      601 gtgtttaaca tttgagcaac ttgttcaggg cggaagctac tcctgggata ttgggatttt
      661 tcgtttggat ctcgctgttc cgcaagcctg ggttgaagag ctggaaaatg gttacgttcc
      721 gccagaaaac tgggagcggg gaatcaatgc gttttatacg tcttattacg taagtcagta
      781 ttacagtgac tataaagcct cgggcaatag taagagcact tatgtgcgtt ttaacagtgg
      841 gctaaatttg ctgggatggc agctgcattc agatgccagt tttagcaaaa cggataataa
      901 tcctggtgag tggaaaagca acacgctgta tctggagcat ggattttctc aaattctcgg
      961 tacgctgcgt attggtgata tgtacacctc agccgatata tttgattccg ttcgcttcac
     1021 cggggttcgg ttatttcgtg atatgcagat gttgccaaac tcgaagcaaa attttacccc
     1081 acgggtacag gggattgccc agagtaacgc gttggtaact attgaacaga acggttttgt
     1141 cgtttatcag aaagaggttc caccaggtcc gttttcgatt agtgatttgc agttagcggg
     1201 cggaggggcg gatcttgatg ttagtgttaa ggaagccgat ggttctgtga ctacgtatct
     1261 ggtgccttat gcggcagtac ctaatatgct gcaacctggt gtatcaaaat atgattttgc
     1321 ggcaggccgt agtcatattg aaggtgcgag caagcaaagt gattttgtcc aggcgggtta
     1381 tcagtatggt tttaataatt tattgacgct gtatggcggc acgatggttg ctaataatta
     1441 ctatgccttt accctcggaa caggttggaa cacgcgtatt ggcgcaattt cagtcgatgc
     1501 aactaaatcg cacagtaagc aagacaatgg tgatgtgttt gacggacaaa gttatcaaat
     1561 tgcctataac aaatttttga gccaaacatc aacacgtttt ggtctggcgg cctggcgtta
     1621 ttcgtcgcgt gattaccgca catttaacga ttatgtgtgg gcaaataata aagataatta
     1681 tcgtcgtgat aaaaacgatg tctatgatat tgccgattat taccagaatg attttggtcg
     1741 caaaaatagc ttttccgcca atatgagtca gtcattgcca gaaggctggg gatctgtgtc
     1801 attaagtact ttatggcgtg attactgggg gcgaagcggc agtagtaaag attatcaact
     1861 gagttattcc aataactggc ggcggataag ctataccctc gcggcaagcc aggcgtatga
     1921 cgagaaccac gccgaagaga aacgctttaa tatttttata tcgattccct ttgactgggg
     1981 cgatgacgtt acgacgccgc gacggcaaat atatatgtct aactcaacga cctttgatga
     2041 tcagggtttt gcttcaaaca ataccggatt atcgggaacc gttgggaacc gcgatcagtt
     2101 taactatggt atcaacttga gccatcaaca tcagggaaat gaaacgacag ctggggccaa
     2161 tttgacctgg accgcaccgg ccgcaacagt gaatggcagc tatagtcagt cgagtactta
     2221 tcgacaggtc ggagccagtg tttcaggggg actggttgcc tggtctggtg gcgttaatct
     2281 ggcgaaccgt ctttccgaaa cgtttgctgt gatgcatgcg ccgggaatca aagatgctta
     2341 tgtcaatggg caaaaatatc gtacaacaaa ctgtaatggt gtggtggtgt acgacggact
     2401 gacaccttat cgggaaaatc acctgatgat ggatgtgtcg caaagcgata gcgaaacaga
     2461 attacgcggg aaccgtaaaa tgaccgcccc ttatcgcggc gcggtcgttc tggttgattt
     2521 tgataccgat cagcgtaagc cctggtttat aaaagcatta agatccgatg gacagccatt
     2581 aacgtttggt tatgaagtca atgatatgca tggtcataac attggtgttg tcggccaggg
     2641 cagtcagata tttattcgca ccaatgaaat accgccagcg gttaatgtgg caattgataa
     2701 gcaacaaggg ctttcatgca caatcacctt cggtaaagag attgatgaaa gtaaaaatta
     2761 tatctgtcgg tgagaggtga aatatgatgt tcagaaatag aatattacta atatttat
//
LOCUS       DBFIFM010000337         2764 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.339, whole genome shotgun sequence.
ACCESSION   DBFIFM010000337 DBFIFM010000000
VERSION     DBFIFM010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.339"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..1265)
                     /locus_tag="N7C74_004728"
     CDS             complement(<1..1265)
                     /locus_tag="N7C74_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HGU2434428.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQSLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFL
                     IMLQQGGQISDSFGGPLSLLTLLKEELLGIRDASESSEESLSDTANALAENARNAGEL
                     GRFMSVARVAAGGGVAVLAALAAAAWQAEQADRALLRSLTLTGGAAATTTAELWKMAG
                     VISDEAGGGIRQAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEG
                     IAADPVKALASLNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQL
                     DKAATPVEKIWDDVKTWTSDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLAL
                     AEFIQSAWETTKNVPGVGALFGELAEENRV"
     gene            complement(1315..1617)
                     /locus_tag="N7C74_004729"
                     /pseudo
     CDS             complement(1315..1617)
                     /locus_tag="N7C74_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            complement(1644..2066)
                     /locus_tag="N7C74_004730"
     CDS             complement(1644..2066)
                     /locus_tag="N7C74_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU2434429.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYIKFVSDAEQQETTKHD
                     VVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEPTGKTEATEPVTSKKHSKAS"
     gene            complement(2080..>2764)
                     /locus_tag="N7C74_004731"
     CDS             complement(2080..>2764)
                     /locus_tag="N7C74_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2434430.1"
                     /translation="GDAYANPLSDDDWQRLAKVKDLTPGEMTAEPYDDNYLDDEDADW
                     TATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFESGDVRAYKIRFPNGTVDVFRGWVS
                     SIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITPATAIKVTPTSGTVAKGKTTTLTV
                     SFEPESATDKTFRAVSADPSKATISVKDMTITVNGVATGKVQIPVVSGNGQFAAVAEV
                     TVTEAGAAG"
BASE COUNT          569 a          881 c          642 g          672 t
ORIGIN      
        1 acgcggttct cttctgccag ttcaccaaac aacgcaccaa cgccgggcac attctttgtc
       61 gtttcccagg ctgactgaat aaattcagcc agcgccagat ccccctgcac aagcagcagc
      121 ttcacctgtt caacggttcc tgccaccacg tcagtgatca gactgagtgc ccccagtgta
      181 tgatcaccta tccatgccca tgcgtcagaa gtccaggttt taacatcgtc ccagattttt
      241 tccaccggcg tggccgcttt atcaagttgc tccagacgtg cattcatgac atccgcaaac
      301 agggacatcg cctccgtcac cgcagcctgt ttacctttcg tgcgctcaag ctcatcaata
      361 tggcgtaact gggaaacgct caggaagtta tactgctgat tcagggaggc cagcgccttc
      421 accggatctg ctgcaatccc ttcaaaggct ttttccacct tcccggcatc gtcccccacc
      481 gtctgcagcc atctctgaga ggtttccccc atgatccgta gctgcccggc ggtatatttc
      541 ccgctttctg ccagacgggc cagattttct gccgcctgtc tgataccacc accggcttca
      601 tcgctgatca ccccggccat tttccacaat tctgccgttg tggtggcagc agcccctccg
      661 gtcagggtca gtgaacgcaa taaggcccgg tcagcctgct ctgcctgcca ggcggcggca
      721 gcaagcgcgg ccagtacggc aaccccgcca cctgccgcca cacgggccac cgacataaat
      781 cgtcccagct caccggcatt ccgggcattt tcagccagtg catttgccgt atctgacagc
      841 gactcctctg atgattcaga ggcatccctg atcccgagaa gttcctcctt cagcagggta
      901 agcaggctga gcggtccacc gaatgaatcg ctgatctgcc ccccctgctg cagcatgata
      961 aggaagggat tctgaccacc ggcaagctga gtgacaatat ccgtgaactg tgcgggcagt
     1021 gtgcgcatgg cagccttata ctgtccgact gatatcccgg ctttttgtgc agccagcgcc
     1081 tgtcggctca gactctgctc agcagcatca gcctgttttc tgaaagactg actgactttc
     1141 ccggacatca aatcagcaag cgcactggtt tcacccagct ctttttttac ccgcgctgcc
     1201 tcttcagaaa aacgggcaga atccagtgtc agcacggctg tcagatcggc aaaattacct
     1261 gccatcgtgg tcacctcctg aaatgtcctc tgataccatc aataactgtc acaacctcct
     1321 tccctcctcc ccgaaacgga ctccaccggc gaggcccgcc gccttctgca tcagtacatc
     1381 attttcgtcc ggcgtctccg cctgtccttc ctctgcctcc ggagcgaaca ggctgaaatc
     1441 cgccggatgc atatccggat cgcaaaaaaa caggctgagt acggcgtaca tcagcccgga
     1501 aaaatgcata tccagctggg tatcctgaaa ataatgcgtg cagtaaaaac gtcgccagtc
     1561 ggcatattcg gtggatgtca tcccggcaag catggcgcgc cagtcggctc tctccatctc
     1621 acgcgccagt ttcaggacaa agttcagctc gccttcgaat gcttttttga tgttaccggc
     1681 tcagtcgctt ctgttttccc ggttggttca ggatcggcat cgtgccggtt atccagcata
     1741 cctgaaagat aaagcacccg gttcgttgcc tgattcagtg catcagcagg ccatcccagc
     1801 atcacttcac ggcggatctg ctgcatctct gtctccggag aggccagagt gcctttgagg
     1861 gaatgggaat gccatagcga catcgccaca agcagggatg ccgtttccag atatcgctgg
     1921 ttaatgtgca cgacatcatg cttcgttgtc tcctgttgtt ctgcgtctga aacaaacttt
     1981 atataatcaa accgctgcag cgcagacaat tcggaaagcg tgacggacac accgttatat
     2041 tcaaattgtt ctgttttcag aaacatgtat tacctccgtt taccctgcag cgcccgcttc
     2101 agtaacggtg acttcagcca ctgcggcgaa ctgaccattt ccgctcacca cagggatctg
     2161 caccttacct gtcgccacgc cgtttaccgt aattgtcata tctttcacac taatggtggc
     2221 tttcgacgga tcggcggaaa ccgctctgaa cgtcttgtcg gttgcacttt ccggctcaaa
     2281 agaaaccgtc agggtggttg ttttcccttt tgccaccgta ccggatgtcg gtgtcacctt
     2341 aattgcagtg gccggcgtaa tttcgctgcg ttcttccgcc acggaaggtt tgcccacgtt
     2401 agtgactttc accgtgcggg tgatcacttc tttcgccgtc acggccttac cgatactgct
     2461 gacccagcca cggaacacat ccaccgtgcc atttgggaaa cggattttat aggcccgcac
     2521 atccccgctt tcaaaccagc ctataagccc tttctgacct tcttctcccg gtttccaggc
     2581 cagcgtaaaa ctggtatctc ctgcagactt ctgcccctgc ccggtcgcgg tccagtccgc
     2641 gtcttcatca tccaggtagt tatcatcgta gggttctgcc gtcatctcgc ccggcgtcag
     2701 atccttcacc ttagccagtc gctgccagtc atcgtctgac aacgggtttg cataagcatc
     2761 accc
//
LOCUS       DBFIFM010000338         2720 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.340, whole genome shotgun sequence.
ACCESSION   DBFIFM010000338 DBFIFM010000000
VERSION     DBFIFM010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.340"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            86..1975
                     /gene="mnmG"
                     /locus_tag="N7C74_004732"
     CDS             86..1975
                     /gene="mnmG"
                     /locus_tag="N7C74_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HGU2434431.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSDDKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            2039..2662
                     /gene="rsmG"
                     /locus_tag="N7C74_004733"
     CDS             2039..2662
                     /gene="rsmG"
                     /locus_tag="N7C74_004733"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HGU2434432.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT          657 a          699 c          726 g          638 t
ORIGIN      
        1 gtctttctca agccgacaaa gttgagtaga atccacggcc cgggcttcaa tccattttca
       61 taccgcgtta tgcgaggcaa tcaccatgtt ttatccggat ccttttgacg tcatcatcat
      121 tggcgggggt catgcaggca ccgaggccgc gatggctgcg gcgcgtatgg gtcaacagac
      181 tctgcttttg acacacaata tcgacactct ggggcagatg agctgcaacc cggcgatcgg
      241 cggtattggg aagggacatc tggtaaaaga agtggatgca ctcggcggtc tgatggcgaa
      301 agcgatcgat caggcgggta tccagtttag gatactaaac gcaagcaagg gaccggcagt
      361 tcgcgctacc cgagctcagg cggatcgtgt gctctaccgt caggcggtac gtacggcgct
      421 ggagaaccaa ccgaacctga tgatcttcca gcaggcggtt gaagatctta ttgtcgaaaa
      481 cgatcgtgtg gtcggtgctg ttacccaaat gggactgaag ttccgtgcca aagccgtcgt
      541 gctcaccgtt gggacgttcc tcgacggtaa aattcatatc ggtctggata actacagcgg
      601 tggccgtgct ggtgatccgc cgtccattcc gctttctcgc cgtttgcgtg aacttccgct
      661 gcgcgttggt cgtctgaaaa ccgggacacc accgcgtatt gatgctcgaa ccatcgactt
      721 tagcgtgctt gcgcaacagc atggcgataa cccaatgccg gtattctctt ttatgggcaa
      781 tgcgtcccag catccacagc aggtgccgtg ttatatcacc cataccaacg agaaaaccca
      841 tgatgtgatc cgcagtaacc tcgatcgtag cccaatgtac gcaggggtga tcgaaggtgt
      901 cggcccacgc tactgcccgt cgatcgaaga caaagtcatg cgcttcgccg acagaaatca
      961 gcatcagatc ttccttgaac cggaagggct gacttctaac gaaatttatc cgaacggtat
     1021 ctccaccagc ctgccgttcg atgtgcagat gcaaatcgtc cgctctatgc aggggatgga
     1081 aaacgcgaag atcgtgcgtc cgggttatgc cattgagtat gacttcttcg atcctcgcga
     1141 cctgaaaccg acgctggaga gcaagtttat ccaggggctg ttctttgctg gtcagattaa
     1201 cggcactacc ggttacgaag aagccgctgc gcaaggtttg ctggccggtc ttaacgctgc
     1261 ccgtctgtct gatgacaaag aaggttgggc tccggcgcgt tctcaggcct atctcggcgt
     1321 actggttgat gacctgtgca ctttaggaac caaagaaccg tatcgtatgt ttacctcgcg
     1381 cgcagaatat cgtctgatgc tgcgcgaaga taatgcggat ctgcgtttga ctgaaatcgg
     1441 tcgtgaactg ggcctggtgg atgacgaacg ttgggcgcgc tttaacgaga aacttgagaa
     1501 tatcgagcgt gagcgtcagc gtctgaaatc gacctgggta accccgtcgg cggaagctgc
     1561 agccgaagtg aatgctcacc tgactgcgcc gctttcccgt gaagccagtg gtgaagatct
     1621 gctgcgtcgt ccggaaatga cttatgaaaa attaaccacg ctgacgccgt ttgcccctgc
     1681 gttgacagac gaacaggcgg cggaacaggt tgagattcag gttaaatacg aaggttatat
     1741 cgcgcgccag caagatgaga tcgaaaagca gctgcgtaac gagaacacct tgctacccgc
     1801 gacactggat taccgccagg tatccggtct ttctaacgaa gtgatcgcca aacttaacga
     1861 tcacaaaccg gcctctatcg gccaggcttc acgtatttct ggcgtcacgc ctgcggccat
     1921 ctccattctg ctggtgtggc tgaaaaaaca gggtatgctg cgtcgtagcg cataacgcat
     1981 taaaaatgcc tggtaagccc ccgcttacca ggcaacgcat caagaacagg taatcaccgt
     2041 gctcaacaaa ctctccttac tgctgaaaga cgcaggtatt tcgcttaccg atcaccagaa
     2101 aaaccagctt attgcctacg tgaatatgct gcataaatgg aacaaagcgt acaacctgac
     2161 ttcggtccgc gatcctaatg agatgctggt acgccatatt ctcgatagca ttgtggtggc
     2221 accgtatctg caaggtgaac ggtttatcga tgtcggcacc ggtcctggac tgcctggcat
     2281 tccactctct atcgtgcgtc ctgaagccca tttcactctg ttggatagcc ttggtaaacg
     2341 cgtgcgtttc cttcgtcagg tgcaacatga gcttaaactg gagaatattg agccagtaca
     2401 gagcagggta gaagagtttc cttcagagcc gccatttgat ggcgtaatta gccgcgcttt
     2461 tgcctctctg aacgatatgg tgagctggtg ccaccatctt cctggtgagc aaggccgttt
     2521 ctacgcgctg aaagggcaaa tgccggaaga tgaaatcgct ttgttgcccg aagaatatca
     2581 ggtcgaatca gtggttaaac ttcaggttcc agccctggat ggcgaacgtc atctggtggt
     2641 gattaaagca aataaaattt aatttttatc aaaaaaatca taaaaaattg accggttaga
     2701 ctgttaacaa caaccaggtt
//
LOCUS       DBFIFM010000339         2694 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.341, whole genome shotgun sequence.
ACCESSION   DBFIFM010000339 DBFIFM010000000
VERSION     DBFIFM010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.341"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            10..>2694
                     /gene="eae"
                     /locus_tag="N7C74_004734"
     CDS             10..>2694
                     /gene="eae"
                     /locus_tag="N7C74_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin type gamma"
                     /protein_id="HGU2434433.1"
                     /translation="MITHGCYTRTRHKHKLKKTLIMLSAGLGLFFYVNQNSFANGENY
                     FKLGSDSKLLTHDSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMM
                     KAAPGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLIYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKSWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTAD
                     KTSAKADNADTITYTATVKKNGVAQANVPVSFNIVSGTATLGANSAKTDANGKATVTL
                     KSSTPGQVVVSAKTAEMTSALNASAVIFFDQTKASITEIKADKTTAVANGKDAIKYTV
                     KVMKNGQPVNNQSVTFSTNFGMFNGKSQTQATTGNDGRATITLTSSSAGKATVSATVS
                     DGAEVKATEVTFFDELKIDNKVDIIGNNVRGELPNIWLQYGQFKLKASGGDGTYSWYS
                     ENTSIATVDASGKVTLNGKGSVVIKATSGDKQTVSYTIKAPSYMIKVDKQAYYADAMS
                     ICKNLLPSTQTVLSDIYDSWGAANKYSHYSSMNSITAWI"
BASE COUNT          784 a          523 c          630 g          757 t
ORIGIN      
        1 agccataaca tgattactca tggttgttat acccggaccc ggcacaagca taagctaaaa
       61 aaaacattga ttatgcttag tgctggttta ggattgtttt tttatgttaa tcagaattca
      121 tttgcaaatg gtgaaaatta ttttaaattg ggttcggatt caaaactgtt aactcatgat
      181 agctatcaga atcgcctttt ttatacgttg aaaactggtg aaactgttgc cgatctttct
      241 aaatcgcaag atattaattt atcgacgatt tggtcgttga ataagcattt atacagttct
      301 gaaagcgaaa tgatgaaggc cgcgcctggt cagcagatca ttttgccact caaaaaactt
      361 ccctttgaat acagtgcact accactttta ggttcggcac ctcttgttgc tgcaggtggt
      421 gttgctggtc acacgaataa actgactaaa atgtccccgg acgtgaccaa aagcaacatg
      481 accgatgaca aggcattaaa ttatgcggca caacaggcgg cgagtctcgg tagccagctt
      541 cagtcgcgat ctctgaacgg cgattacgcg aaagataccg ctcttggtat cgctggtaac
      601 caggcttcgt cacagttgca ggcctggtta caacattatg gaacggcaga ggttaatctg
      661 cagagtggta ataactttga cggtagttca ctggacttct tattaccgtt ctatgattcc
      721 gaaaaaatgc tggcatttgg tcaggtcgga gcgcgttaca ttgactcccg ctttacggca
      781 aatttaggtg cgggtcagcg ttttttcctt cctgcaaaca tgttgggcta taacgtcttc
      841 attgatcagg atttttctgg tgataatacc cgtttaggta ttggtggcga atactggcga
      901 gactatttca aaagtagcgt taacggctat ttccgcatga gcggctggca tgagtcatac
      961 aataagaaag actatgatga gcgcccagca aatggcttcg atatccgttt taatggctat
     1021 ctaccgtcat atccggcatt aggcgccaag ctgatatatg agcagtatta tggtgataat
     1081 gttgctttgt ttaattctga taagctgcag tcgaatcctg gtgcggcgac cgttggtgta
     1141 aactatactc cgattcctct ggtgacgatg gggatcgatt accgtcatgg tacgggtaat
     1201 gaaaatgatc tcctttactc aatgcagttc cgttatcagt ttgataaatc gtggtctcag
     1261 caaattgaac cacagtatgt taacgagtta agaacattat caggcagccg ttacgatctg
     1321 gttcagcgta ataacaatat tattctggag tacaagaagc aggatattct ttctctgaat
     1381 attccgcatg atattaatgg tactgaacac agtacgcaga agattcagtt gatcgttaag
     1441 agcaaatacg gtctggatcg tatcgtctgg gatgatagtg cattacgcag tcagggcggt
     1501 cagattcagc atagcggaag ccaaagcgca caagactacc aggctatttt gcctgcttat
     1561 gtgcaaggtg gcagcaatat ttataaagtg acggctcgcg cctatgaccg taatggcaat
     1621 agctctaaca atgtacagct tactattacc gttctgtcga atggtcaagt tgtcgaccag
     1681 gttggggtaa cggactttac ggcggataag acttcggcta aagcggataa cgccgatacc
     1741 attacttata ccgcgacggt gaaaaagaat ggggtagctc aggctaatgt ccctgtttca
     1801 tttaatattg tttcaggaac tgcaactctt ggggcaaata gtgccaaaac ggatgctaac
     1861 ggtaaggcaa ccgtaacgtt gaagtcgagt acgccaggac aggtcgtcgt gtctgctaaa
     1921 accgcggaga tgacttcagc acttaatgcc agtgcggtta tattttttga tcaaaccaag
     1981 gccagcatta ctgagattaa ggctgataag acaactgcag tagcaaatgg taaggatgct
     2041 attaaatata ctgtaaaagt tatgaaaaac ggtcagccag ttaataatca atccgttaca
     2101 ttctcaacaa actttgggat gttcaacggt aagtctcaaa cgcaagcaac cacgggaaat
     2161 gatggtcgtg cgacgataac actaacttcc agttccgccg gtaaagcgac tgttagtgcg
     2221 acagtcagtg atggggctga ggttaaagcg actgaggtca ctttttttga tgaactgaaa
     2281 attgacaaca aggttgatat tattggtaac aatgtcagag gcgagttgcc taatatttgg
     2341 ctgcaatatg gtcagtttaa actgaaagca agcggtggtg atggtacata ttcatggtat
     2401 tcagaaaata ccagtatcgc gactgtcgat gcatcaggga aagtcacttt gaatggtaaa
     2461 ggcagtgtcg taattaaagc cacatctggt gataagcaaa cagtaagtta cactataaaa
     2521 gcaccgtcgt atatgataaa agtggataag caagcctatt atgctgatgc tatgtccatt
     2581 tgcaaaaatt tattaccatc cacacagacg gtattgtcag atatttatga ctcatggggg
     2641 gctgcaaata aatatagcca ttatagttct atgaactcaa taactgcttg gatt
//
LOCUS       DBFIFM010000340         2683 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.342, whole genome shotgun sequence.
ACCESSION   DBFIFM010000340 DBFIFM010000000
VERSION     DBFIFM010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.342"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            1..541
                     /locus_tag="N7C74_004735"
                     /pseudo
     CDS             1..541
                     /locus_tag="N7C74_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
     gene            complement(558..878)
                     /locus_tag="N7C74_004736"
     CDS             complement(558..878)
                     /locus_tag="N7C74_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HGU2434434.1"
                     /translation="MAEKQVKDYDKFNLRFPDGMRDAIAERAKRNGRSMNSEIVQILE
                     DALNAENTLGEIADKINSVSVPLNVDALVQLQAQVIAMQKEIQEKFREQNEKLRELLN
                     KKPT"
     gene            986..1159
                     /locus_tag="N7C74_004737"
     CDS             986..1159
                     /locus_tag="N7C74_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HGU2434435.1"
                     /translation="MQDTLFTERKNIKLNLRLPSRLNEDLRRLAEMDCISLNSAIVRL
                     LAKGVREEVANGR"
     gene            1230..2153
                     /locus_tag="N7C74_004738"
     CDS             1230..2153
                     /locus_tag="N7C74_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HGU2434436.1"
                     /translation="MTSLAIADRTINVPFHGTNLFLVGINNEPYVPMKPVVEGMGMVW
                     AAQFVKLKQRFVKGISEIEIPSAGGKQLMTCLAFRKFAAWLSSIQPNKVRPEIRDKVI
                     QYQEECDDVLYEYWTKGHVINPRKAKKALPGKITTEQQEAIKQLVMSRGQSLPKEKQA
                     KAMITMWSSLKSHFGCSYKEISEEQFTEALSLAARVPLEGELIGKQEKSTNELSAKEA
                     NSLVWLWDYANRSQALFRELYPALKQIQSNYSGRCYDYGHEFSYVIGMARDVLINHTR
                     DVDINEPDGPTNLSAWVRLKNKELPPSLHRY"
     gene            2208..>2683
                     /locus_tag="N7C74_004739"
     CDS             2208..>2683
                     /locus_tag="N7C74_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU2434437.1"
                     /translation="MLYSNILAHARRCAPAESCGFVVRTPEGERYIPCVNISAEPEAY
                     FRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGDIHKFR
                     CVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMAVTG"
BASE COUNT          671 a          605 c          722 g          685 t
ORIGIN      
        1 ggcagtggct ttgagatgaa cggaaagggc agcagtgccc gcccgtcgct gacggtgtcc
       61 aatctgttcg gtcttgtcac cgggatggcg gaggatttgc agagcctggt gggtgccacg
      121 gtggtccgcc gccgggtgta tgcccgtttt ctggatgcgg tgaattttgt ggcgggcggt
      181 cgtggttatc gggcggcaga ggtaaaaaga ataaaaaaat cccgcagagt tgcggggaca
      241 gacaaagatt aacgttaaga tgtcatcgct gacggccatg acggcctcgt ttgtgctggc
      301 cacaccgacc gagacggacg gggcgctgtt tcccggtcgc atcatgctgg cgaacacctg
      361 tatgtgggat taccggggag atgaatgcgg gtataacggt cctgcggtgg cggatgagtt
      421 cgacaacccc accacggata tccgtaagga cagatgcagc aagtgcatgc gcgggtgtga
      481 actgcgcagg aatgtcggca attttggcgg tttcctttcc attaataaac tttcgcagta
      541 atggattatg cccaccgtca ggtgggtttt ttatttagta gttctctcaa cttttcgttc
      601 tgctctctga acttttcctg tatttctttt tgcatggcga taacctgggc ttgaagttga
      661 actagcgcat caacatttag cggaaccgag acgctgttaa ttttatctgc tatttccccg
      721 agtgtatttt ctgcattcaa ggcatcttcc agtatctgaa caatctctga gttcatagag
      781 cgcccgtttc gtttggctcg ttcagctata gcatctcgca ttccgtcagg aaaacggagg
      841 ttgaacttgt cgtagtcttt tacttgtttt tcggccattg caaatctctc aaaaaaaatc
      901 atggtgccat attgccatac gatttcaatg gtggcattat ggccttcatg gcgtcacttt
      961 ggcaccaaat aaaggagatt agataatgca agatacactt ttcactgagc gcaaaaatat
     1021 caaactcaac cttcgccttc catcacggct gaatgaagac cttcgccgcc tggcggaaat
     1081 ggactgtata tctctgaact ctgcaattgt tcgtttgctg gcaaaaggtg ttagggaaga
     1141 ggtggcgaat ggtcgctaaa aacagcgaag cctcaatggc tgcaaccatt gaggcttcta
     1201 aattaccagt taaccacgag aaaactgata tgactagttt agcaattgca gatcgcacaa
     1261 tcaatgttcc attccacgga acaaatctct ttttggttgg aattaacaat gagccttatg
     1321 ttcctatgaa gcctgttgtt gaaggtatgg ggatggtttg ggctgctcaa tttgttaagt
     1381 taaaacagag gtttgtcaaa ggtatttcgg aaatcgaaat accttctgct ggcggtaaac
     1441 agttaatgac atgtcttgcc tttcgtaaat ttgcggcttg gctttcaagc attcaaccaa
     1501 acaaagtccg ccctgaaatc cgcgacaagg taatccagta tcaggaagag tgtgacgatg
     1561 tgctctacga gtactggact aaaggccatg tgattaaccc gcgcaaagct aaaaaggcat
     1621 tgccggggaa aatcaccact gaacagcagg aagccattaa acaactcgtc atgagtcgcg
     1681 gtcagtctct gccaaaagaa aaacaggcga aggcgatgat caccatgtgg tcgtcactga
     1741 aatctcattt tgggtgttca tacaaagaaa tcagcgagga gcagtttacc gaagctctgt
     1801 cacttgctgc tcgcgttccg cttgaaggag agttaatcgg caaacaagag aagagcacca
     1861 acgagctttc cgcaaaagaa gcaaacagcc ttgtatggtt atgggattat gctaaccgct
     1921 cacaggcatt attccgcgaa ctgtatccgg cgctaaaaca aattcaatcg aactattccg
     1981 gcagatgcta cgactacggt catgagtttt cgtatgttat cggaatggcg agagatgttt
     2041 taatcaacca cacacgagat gttgatatta atgagccaga cggaccaacg aacctttccg
     2101 catgggtaag gcttaagaac aaagagttgc cgccttcact gcatcgctac tgacaattga
     2161 caacttaaca aacccagctt cggctgggtt ttttatcagg agttctcatg ctctatagca
     2221 atatattggc gcacgcccgg cgatgtgcgc cagcggagtc gtgcggcttc gtggtgagaa
     2281 cgccggaggg ggagcggtat atcccttgtg tgaatatctc tgcagagccg gaggcgtatt
     2341 ttcgtattgc accggaagac tggctgcggg cagagatgca gggggagatt gtggcactgg
     2401 tccacagtca tcccggtggt ctgccctggc tgagcgaggc cgaccggcgg ctgcagataa
     2461 aaagtgcact gtcctggtgg ctggtctgcc ggggggacat tcataaattc cgctgtgtgc
     2521 cacatctgac aggacggcgc tttgagcacg gggtgacgga ctgttacacg ctgttccggg
     2581 atgcatacca tctggcggga attgatatgc cggattttga gcgtgaggat gactggtggc
     2641 gtaacggtca gaacctttac ctggacaata tggcggtcac cgg
//
LOCUS       DBFIFM010000341         2671 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.343, whole genome shotgun sequence.
ACCESSION   DBFIFM010000341 DBFIFM010000000
VERSION     DBFIFM010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.343"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..148)
                     /locus_tag="N7C74_004740"
     CDS             complement(<1..148)
                     /locus_tag="N7C74_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HGU2434438.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCGLTNVGEQRGTYILKPLSMKGNL
                     TAEKI"
     gene            complement(161..2611)
                     /locus_tag="N7C74_004741"
                     /pseudo
     CDS             complement(161..2611)
                     /locus_tag="N7C74_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT          749 a          553 c          536 g          833 t
ORIGIN      
        1 caattttttc ggcggtgaga ttacctttca tactcagtgg tttgagtata tatgttccac
       61 gttgttcccc gacattggtc aatccacagg gagcactttg cacaataaaa gaatatagac
      121 tcaatgtgat taataaaagc cacttcatag taaactcctt ttactggcat atatatgttt
      181 tgttttcatc gataacgtgt tgaaaagtaa tagtacagaa taaattctgt tcatcattga
      241 gagcaacttt tatgccagtt ggaatttcat ctacgcgaat aaaaaggcga ctaccctggc
      301 caacgactcc aatattgttt tcctggagat ccagtacgtc atagccaaaa gttaatggcg
      361 aaccatcggg acgcagtgcc tgtatatacc agggcttacg ttggtcagtt gtaaactgca
      421 cataagaaac tgctccacgg taaggcgcaa taatttgacg attgccctgc aattctgttt
      481 cactttcact gtttgctgta tcaagtacca gatggttttc acgatgaggt atcatcaggt
      541 cgtaaacaac ctggcctttg ctgtttgttc ggttgtattt ttgtccatta atatgcgcgc
      601 cttctaaccc aggcgcatcc agaattgcaa atgtatcgga tagctggtta gtgatattaa
      661 taccaccggg ccagacaact aatccactac taatacttcc accactttgc taggcgtttt
      721 tagaatggct atagctgcca tctattgtgg cgatgggagt tctccagctt aaattcgtac
      781 caagcgatgt atcattattt tgttgttgct gattaacata tataccataa tttaactgat
      841 catgttcacc ggctatgcca gtaattccag tattgttgga ggaatagcca tctttggaaa
      901 atgaggtcga attcgataag ttaatttggt gacgtgtttt ggcaatatca tccccccagt
      961 agaaaggaat ggagataaac agattaaaac gctcctcttc tttgtcgttt tcatcatagg
     1021 attggctggc agagaaagta taactgatgc gttgccagct attggaataa ctgaattggt
     1081 aatctttagc atttccactt cgcccccagt aattccgcca aagcgcactt aatgatacat
     1141 ttcccagatt attggataaa ggttgcatga tattggcaga aaggctattt tttctgccaa
     1201 tatcataaaa atcgtcatag tcactctgat gatttatttt atcattttcg taaagatggt
     1261 cgctgaatgt cctgtaatcc tgcgaagcat aacgccaggc ggccacgcta aatcgagtgg
     1321 ccgtttgtac tagatattta ttataggcga cctgatagct cgtaccttcg tgtgttatat
     1381 cattatttaa cttactactc gatcgtgtag cgtcaaaaga gatggcaccc agcggtgtat
     1441 tccaaccatt tcccagagtt atagcattat agttatcgga taaaatcgta ccgccataaa
     1501 gtgtcaaaag attgttgagg ccatatatat aatttgcctc gagaaagtcc tcctgattct
     1561 ttacaccata tatcttactg cgtccggcga taaaatcgaa atttgaaata ccaggttgca
     1621 gcatgttagg cacggaagag tagggcacca aaaaggaacg aacactgcca tctgcttcct
     1681 ttatgctgac atcgagatca gagccgctgc ccgagagttg caaatctgcg atagtaaatg
     1741 gtcccggggg aacctctttt tggtaaatga tgtagccatt ttgcgaaacc gttatcaagg
     1801 cattactttg cgcaacacct tgtactaaag gtgtaaagct ctgcatagaa tcaggtagca
     1861 tttgcatatc gcgaaataag cgtattccgc taaatctcag agagtcgaat atgagtgagg
     1921 atgtgtaatt ttctcctatc tgaactgtgc ttaatatctg cgaccagcca tgttccaggt
     1981 aaagagtatt actttgccat gtgcctttca tatcatcggg tttgctatag ctggcatcag
     2041 agtgtaactg ccaactaaat aaatttaatc cactgttaaa tcggccataa ctggctgtat
     2101 tactgttatt attgctgtca taagagcgat actgacttaa attataggag gtataaaaag
     2161 catcaatgcc gcgatcccaa ctttccgggg gaacgtaacc tctttcaagt tcattgatat
     2221 acgcctgtgg tactgttaaa tttagtcggt gttcactgat atcccactga tattgtccac
     2281 catgaaccgc tttatccagt aaaatacaat tatcttctgt attgaggtta ccggtcttta
     2341 cccccagttt ggttagtagc accttaggca gacatggttt gactggtgac tctgggatcg
     2401 tgatatcttg ttttccacgc cactggttat ttacataaaa atcaagttcg tagtttcctg
     2461 gaagcggttg tttattatcg aaatgatatt cggaaacttt ttctcctttc atgccgccga
     2521 tcataaagtg cgtatcaaaa gtttcttcag cataacccgg aaaagaatac aaaaaaataa
     2581 tagcagcgac ccatcgtgtc gtccttaaca tgactttttc ctgttatctc gcggcgattt
     2641 tactatggat gttattcccg taatcatcaa c
//
LOCUS       DBFIFM010000342         2649 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.344, whole genome shotgun sequence.
ACCESSION   DBFIFM010000342 DBFIFM010000000
VERSION     DBFIFM010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.344"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            89..1054
                     /gene="dusB"
                     /locus_tag="N7C74_004742"
     CDS             89..1054
                     /gene="dusB"
                     /locus_tag="N7C74_004742"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HGU2434439.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1080..1376
                     /gene="fis"
                     /locus_tag="N7C74_004743"
     CDS             1080..1376
                     /gene="fis"
                     /locus_tag="N7C74_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HGU2434440.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1461..2345
                     /gene="yhdJ"
                     /locus_tag="N7C74_004744"
     CDS             1461..2345
                     /gene="yhdJ"
                     /locus_tag="N7C74_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HGU2434441.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLLEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLITK"
     gene            2429..2608
                     /locus_tag="N7C74_004745"
     CDS             2429..2608
                     /locus_tag="N7C74_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HGU2434442.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
BASE COUNT          730 a          614 c          654 g          651 t
ORIGIN      
        1 ggattggtca aagtttggcc tttcatctcg tgcaaaaaat gcgtaatata cgccgccttg
       61 cagtcacagt atggtcattt cttaactcat gcgcatcgga caatatcagc tcagaaatcg
      121 cctgatcgca gcgcccatgg ctggcattac agacagacct tttcggacgt tgtgctacga
      181 gatgggagcc ggattgacag tatccgagat gatgtcttct aacccacagg tttgggaaag
      241 cgacaaatct cgtttacgga tggtgcacat tgatgaaccc ggtattcgca ccgtgcaaat
      301 tgctggtagc gatccgaaag aaatggcaga tgcagcacgt attaacgtgg aaagcggtgc
      361 ccagattatt gatatcaata tgggttgccc ggctaaaaaa gtgaatcgca agctcgcagg
      421 ttcagccctc ttgcagtacc cggatgtcgt taaatcgatc cttaccgagg tcgtcaatgc
      481 agtggacgtt cctgttaccc tgaagattcg caccggctgg gcaccggaac accgtaactg
      541 cgaagagatt gcccaactgg ctgaagactg tggcattcag gctctgacca ttcatggccg
      601 tacacgcgcc tgtttgttca atggagaagc tgagtacgac agtattcggg cagttaagca
      661 gaaagtttcc attccggtta tcgcgaatgg cgacattact gacccgctta aagccagagc
      721 tgtgctcgac tatacagggg cggatgccct gatgataggc cgcgcagctc agggaagacc
      781 ctggatcttt cgggaaatcc agcattatct ggacactggg gagttgctgc ccccgctgcc
      841 tttggcagag gttaagcgct tgctttgcgc gcacgttcgg gaactgcatg acttttatgg
      901 tccggcaaaa gggtaccgaa ttgcacgtaa acacgtttcc tggtatctcc aggaacacgc
      961 tccaaatgac cagtttcggc gcacattcaa cgccattgag gatgccagcg aacagctgga
     1021 ggcgttggag gcatacttcg aaaattttgc gtaaacagaa ataaagagct gacagaacta
     1081 tgttcgaaca acgcgtaaat tctgacgtac tgaccgtttc taccgttaac tctcaggatc
     1141 aggtaaccca aaaacccctg cgtgactcgg ttaaacaggc actgaagaac tattttgctc
     1201 aactgaatgg tcaggatgtg aatgacctct atgagctggt actggctgaa gtagaacagc
     1261 ccctgttgga catggtgatg caatacaccc gtggtaacca gacccgtgct gcgctgatga
     1321 tgggcatcaa ccgtggtacg ctgcgtaaaa aattgaaaaa atacggcatg aactaattca
     1381 ggttagctaa ttgcttgatt aaaaggcgct actcggcatg gggaagcgcc ttttttatag
     1441 gtgtcacaaa gggagtgacc atgagaacag gatgtgaacc gacccggttt ggtaatgaag
     1501 ctaagaccat tattcacggt gatgcccttg ccgaacttaa aaagctacct actgaaagcg
     1561 tcgatctgat ctttgccgac ccaccgtata acatcggtaa aaattttgat ggtctgatcg
     1621 aagcctggaa agaagatctg tttatcgact ggctgttaga agtgattgca gagtgccacc
     1681 gcgttctgaa aaagcagggc agcatgtaca tcatgaacag tacggaaaac atgcccttta
     1741 tcgatctcca gtgccgcaag ctttttacca tcaaaagtcg catcgtctgg tcatatgaca
     1801 gttctggagt gcaggcgaaa aaacactacg gctccatgta cgaacccatc ctgatgatgg
     1861 tgaaagacgc aaagaactac acattcaacg gtgatgctat tctggtagaa gccaaaaccg
     1921 gatcgcagcg cgcgttgatc gattatcgca aaaatcctcc acagccttac aatcatcaaa
     1981 aagtaccggg taacgtctgg gattttccgc gcgtgcgtta tttgatggat gaatatgaaa
     2041 accacccgac gcaaaaaccg gaagccttac tgaaacgcat tattctcgcc tcttccaacc
     2101 caggcgatat cgttctcgac ccgtttgctg gtagctttac taccggtgcc gtagccatcg
     2161 ccagcggacg aaaattcatt ggtattgaga tcaacagcga gtacatcaaa atggggcttc
     2221 gacggctgga tgtcgcgtcg cattactctg cggaagaact ggcgaaagtg aaaaaaagaa
     2281 agaccggcaa cctgtcaaaa cgaagccggt taagcgaagt tgaccccgat ctcattacaa
     2341 agtaaaggat gtgtaagcct ggtttgcaga ttattcattt cgtgtatatt tcctgccaga
     2401 cttggttaaa catgcacagg ctcaggtaat gattcgcaag tattggtggc tcgtcgtttt
     2461 cgctgtcttc gtttttctgt ttgatacttt actgatgcag tggattgaac tgctggcaac
     2521 agaaacagac aaatgccgca atatgaactc agttaatcca ctaaaactgg ttaactgtga
     2581 cgaactgaat tttcaggaca gaatgtgaat ttacatgaca cttaattcat tcgtttgatg
     2641 aattaattt
//
LOCUS       DBFIFM010000343         2604 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.345, whole genome shotgun sequence.
ACCESSION   DBFIFM010000343 DBFIFM010000000
VERSION     DBFIFM010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.345"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            19..2166
                     /locus_tag="N7C74_004746"
     CDS             19..2166
                     /locus_tag="N7C74_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HGU2434443.1"
                     /translation="MLYNIPCRIYILSTLSLCISGIVSTATATSSETKISNEETLVVT
                     TNRSASNLWESPATIQVIDQQTLQNSTNASIADNLQDIPGVEITDNSLAGRKQIRIRG
                     EASSRVLILIDGQEVTYQRAGDNYGVGLLIDESALERVEVVKGPYSVLYGSQAIGGIV
                     NFITKKGGDKLASGVVKAVYNSATAGWEESIAVQGSIGGFDYRINGSYSDQGNRDTPD
                     GRLPNTNYRNNSQGVWLGYNSGNHRFGLSLDRYRLATQTYYEDPDGSYEAFSVKIPKL
                     EREKVGVFYDTDVDGDYLKKIHFDAYEQTIQRQFANEVKTTQPVPSPMIQALTVHNKT
                     DTHDKQYTQAVTLQSHFSLPANNELVTGAQYKQDRVSQRSGGMTSSKSLTGFINKETR
                     TRSYYESEQSTVSLFAQNDWQFADHWTWTMGVRQYWLSSKLTRGDGVSYTAGIISDTS
                     LARESASDHEMVTSTSLRYSGFDNLELRAAFAQGYVFPTLSQLFMQTSAGGSVTYGNP
                     DLKAEHSNNFELGARYNGNQWLIDSAVYYSEAKDYIASLICDGSIVCNGNTNSSRSSY
                     YYYDNIDRAKTWGLEISAEYNGWVFSPYISGNLIRRQYETSTLKTTNTGEPAINGRIG
                     LKHTLVMGQANIISDVFIRAASSAKDDSNGTETNVPGWATLNFAVNTEFGNEDQYRIN
                     LALNNLTDKRYRTAHETIPAAGFNAAIGFVWNF"
     gene            2475..2604
                     /locus_tag="N7C74_004747"
                     /pseudo
     CDS             2475..2604
                     /locus_tag="N7C74_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vimentin yjdA"
BASE COUNT          758 a          574 c          590 g          682 t
ORIGIN      
        1 gtattaatga gggatgaaat gttatataat ataccttgtc gaatttatat cctttccact
       61 ctgtcattat gcatttctgg gatagtttct actgcaaccg caacttcttc agaaacaaaa
      121 atcagcaacg aagagacgct cgtcgtgacc acgaatcgtt cggcaagcaa cctttgggaa
      181 agcccggcga ctatacaggt tattgaccaa caaacattgc agaactccac caatgcctcc
      241 atagccgata atttgcagga catccccgga gtagagataa cagacaactc cttggcaggc
      301 cgtaaacaaa tccgcattcg tggcgaagca tcctcccgtg ttttaattct cattgatggt
      361 caggaggtaa cttatcagcg cgccggagat aattatggtg tgggactgtt gatagatgag
      421 tctgcgctgg agcgtgttga ggtagtgaaa ggtccatatt ccgtactgta cggttcacag
      481 gcaattggcg gtattgttaa cttcatcacc aaaaagggag gtgacaaact tgcatctgga
      541 gttgtgaaag ctgtttataa ttccgcaaca gcaggctggg aagaatcaat cgcggtccag
      601 gggagcatcg gtggatttga ttatcgcatc aacggtagtt attctgatca gggcaatcgt
      661 gatacgccgg atggacgtct gccgaatacc aactatcgta acaatagtca gggtgtatgg
      721 ttgggttata actccggaaa ccatcgtttt ggcctctcgc ttgatcgcta cagactcgcg
      781 acacaaactt actatgagga tccagacgga agttatgagg catttagtgt caaaatacct
      841 aaacttgaac gagagaaagt tggggtattc tatgacacag acgtggacgg tgactatcta
      901 aaaaaaattc atttcgacgc gtatgagcag accatccagc gccaatttgc caacgaagta
      961 aaaacgacac agcctgttcc cagtccgatg attcaggctc tgaccgttca taacaagact
     1021 gacacccatg ataagcaata cactcaggcg gtcacattgc agagtcactt ttcgctgcct
     1081 gctaataatg aacttgttac cggtgcacag tacaaacaag acagggtcag ccaaaggtcc
     1141 ggtggcatga cctcaagcaa atctctgacc ggcttcatta ataaggaaac acgaactcgc
     1201 tcctattatg agtcagagca aagtacagtc tcactattcg cacaaaatga ctggcaattc
     1261 gccgatcact ggacatggac aatgggagtt cgccaatact ggctttcttc aaagttgacg
     1321 cgtggtgacg gagtatcata taccgcaggc attataagcg atacctctct tgccagagag
     1381 tctgcgagtg atcacgaaat ggtaacatct acaagcctgc gctattcagg tttcgataac
     1441 ctggagttac gcgctgcgtt cgcgcaaggc tacgtatttc ccacactctc ccagcttttt
     1501 atgcagacat ctgcgggcgg cagtgtcaca tacggaaatc ctgatcttaa ggctgaacac
     1561 tccaataact ttgaattagg tgcacgatat aatggtaatc agtggctgat tgacagcgca
     1621 gtttactact cagaagctaa agattatatt gcaagtctga tctgtgatgg cagtatagtt
     1681 tgcaatggta acaccaactc ctcccgtagt agctactatt attatgacaa tattgatcgg
     1741 gcaaaaacat ggggactgga aataagcgcg gaatataatg gctgggtttt ctcgccatat
     1801 atcagtggca atttaattcg tcggcaatat gaaacttcaa cattaaaaac aactaataca
     1861 ggtgaaccag cgataaacgg acgtataggg ctgaaacata ctcttgtgat gggtcaggcc
     1921 aacataatct ctgatgtttt tattcgtgct gcctctagtg caaaagatga cagtaacggt
     1981 accgaaacaa atgttccggg ctgggccact ctcaactttg cagtaaatac agaattcggt
     2041 aacgaggatc agtaccggat taacctggca ctcaataacc tgacagacaa acgctaccgt
     2101 acagcacatg aaactattcc tgcagcaggt tttaatgcag ctataggttt tgtatggaat
     2161 ttctgatgac aaaaacaatc gttatggaat tctttgtcct gatctgcccg ctttgcgggc
     2221 aggctaacga acgtattgtg tttgttgaag gtttttcgac gttgttttcc atctgtgggt
     2281 gaatatttat tcttcccgcc agcatgactc tatactcatt gctacgacaa tgacttataa
     2341 agctggttac tgaacctata cctttgtaat tcaaaagtga tatcagttca caacgcaata
     2401 ctatttttag attatttact ctcacccaga tactgaatca atggataata ccttttgatt
     2461 tatcccgacg cggaagaagg tctcagtccg gattatgggg gaaaatttct caatggtccg
     2521 gtatcaacaa ccagggccgg gaggattaca ggaaacgaga ttttgccccg gacagccccg
     2581 aaataaaatt cagtgatcgc aggg
//
LOCUS       DBFIFM010000344         2571 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.346, whole genome shotgun sequence.
ACCESSION   DBFIFM010000344 DBFIFM010000000
VERSION     DBFIFM010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.346"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..2429
                     /locus_tag="N7C74_004748"
     rRNA            <1..2429
                     /locus_tag="N7C74_004748"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          669 a          567 c          805 g          530 t
ORIGIN      
        1 aaaagaaccc cggcgagggg agtgaaaaag aacctgaaac cgtgtacgta caagcagtgg
       61 gagcacagag caatctgtgt gactgcgtac cttttgtata atgggtcagc gacttatatt
      121 ctgtagcaag gttaaccgaa taggggagcc gaagggaaac cgagtcttaa ctgggcgtta
      181 agttgcaggg tatagacccg aaacccggtg atctagccat gggcaggttg aaggttgggt
      241 aacactaact ggaggaccga accgactaat gttgaaaaat tagcggatga cttgtggctg
      301 ggggtgaaag gccaatcaaa ccgggagata gctggttctc cccgaaagct atttaggtag
      361 cgcctcgtga attcatctcc gggggtagag cactgtttcg gcaagggggt catcccgact
      421 taccaacccg atgcaaactg cgaataccgg agaatgttat cacgggagac acacggcggg
      481 tgctaacgtc cgtcgtgaag agggaaacaa cccagaccgc cagctaaggt cccaaagtca
      541 tggttaagtg ggaaacgatg tgggaaggcc cagacagcca ggatgttggc ttagaagcag
      601 ccatcattta aagaaagcgt aatagctcac tggtcgagtc ggcctgcgcg gaagatgtaa
      661 cggggctaaa ccatgcaccg aagctgcggc agcgacactg tgtgttgttg ggtaggggag
      721 cgttctgtaa gcctgtgaag gtgtgctgtg aggcatgctg gaggtatcag aagtgcgaat
      781 gctgacataa gtaacgataa agcgggtgaa aagcccgctc gccggaagac caagggttcc
      841 tgtccaacgt taatcggggc agggtgagtc gacccctaag gcgaggccga aaggcgtagt
      901 cgatgggaaa caggttaata ttcctgtact tggtgttact gcgaaggggg gacggagaag
      961 gctatgttgg ccgggcgacg gttgtcccgg tttaagcgtg taggctggtt ttccaggcaa
     1021 atccggaaaa tcaaggctga ggcgtgatga cgaggcacta cggtgctgaa gcaacaaatg
     1081 ccctgcttcc aggaaaagcc tctaagcatc aggtaacatc aaatcgtacc ccaaaccgac
     1141 acaggtggtc aggtagagaa taccaaggcg cttgagagaa ctcgggtgaa ggaactaggc
     1201 aaaatggtgc cgtaacttcg ggagaaggca cgctgatatg taggtgaagt ccctcgcgga
     1261 tggagctgaa atcagtcgaa gataccagct ggctgcaact gtttattaaa aacacagcac
     1321 tgtgcaaaca cgaaagtgga cgtatacggt gtgacgcctg cccggtgccg gaaggttaat
     1381 tgatggggtc agcgcaagcg aagctcttga tcgaagcccc ggtaaacggc ggccgtaact
     1441 ataacggtcc taaggtagcg aaattccttg tcgggtaagt tccgacctgc acgaatggcg
     1501 taatgatggc caggctgtct ccacccgaga ctcagtgaaa ttgaactcgc tgtgaagatg
     1561 cagtgtaccc gcggcaagac ggaaagaccc cgtgaacctt tactatagct tgacactgaa
     1621 cattgagcct tgatgtgtag gataggtggg aggctttgaa gtgtggacgc cagtctgcat
     1681 ggagccgacc ttgaaatacc accctttaat gtttgatgtt ctaacgtgga cccgtgatcc
     1741 gggttgcgga cagtgtctgg tgggtagttt gactggggcg gtctcctcct aaagagtaac
     1801 ggaggagcac gaaggttggc taatcctggt cggacatcag gaggttagtg caatggcata
     1861 agccagcttg actgcgagcg tgacggcgcg agcaggtgcg aaagcaggtc atagtgatcc
     1921 ggtggttctg aatggaaggg ccatcgctca acggataaaa ggtactccgg ggataacagg
     1981 ctgataccgc ccaagagttc atatcgacgg cggtgtttgg cacctcgatg tcggctcatc
     2041 acatcctggg gctgaagtag gtcccaaggg tatggctgtt cgccatttaa agtggtacgc
     2101 gagctgggtt tagaacgtcg tgagacagtt cggtccctat ctgccgtggg cgctggagaa
     2161 ctgagggggg ctgctcctag tacgagagga ccggagtgga cgcatcactg gtgttcgggt
     2221 tgtcatgcca atggcactgc ccggtagcta aatgcggaag agataagtgc tgaaagcatc
     2281 taagcacgaa acttgccccg agatgagttc tccctgaccc tttaagggtc ctgaaggaac
     2341 gttgaagacg acgacgttga taggccgggt gtgtaagcgc agcgatgcgt tgagctaacc
     2401 ggtactaatg aaccgtgagg cttaacctta caacgccgaa ggtgttttgg cggatgagag
     2461 aagattttcg gcctgataca gattaaatca gaacgcagaa gcggtctgat aaaacagaat
     2521 ttgcctggcg gccttagcgc ggtggtccca cctgacccca tgccgaactc a
//
LOCUS       DBFIFM010000345         2517 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.347, whole genome shotgun sequence.
ACCESSION   DBFIFM010000345 DBFIFM010000000
VERSION     DBFIFM010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.347"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            351..494
                     /locus_tag="N7C74_004749"
     CDS             351..494
                     /locus_tag="N7C74_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HGU2434444.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            507..716
                     /gene="cspE"
                     /locus_tag="N7C74_004750"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             507..716
                     /gene="cspE"
                     /locus_tag="N7C74_004750"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HGU2434445.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            882..1691
                     /gene="rlmA"
                     /locus_tag="N7C74_004751"
     CDS             882..1691
                     /gene="rlmA"
                     /locus_tag="N7C74_004751"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HGU2434446.1"
                     /translation="MSFSCPLCHQPLTREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLNEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQKSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQILAAKEVLDCQTDFNIHLWQRSY"
     gene            complement(1688..2254)
                     /gene="mntP"
                     /locus_tag="N7C74_004752"
     CDS             complement(1688..2254)
                     /gene="mntP"
                     /locus_tag="N7C74_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HGU2434447.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(2251..2373)
                     /locus_tag="N7C74_004753"
     CDS             complement(2251..2373)
                     /locus_tag="N7C74_004753"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434448.1"
                     /translation="MHINRYACMNAKTHRGLLFMYACLLGAMMCFMDTPVRTLS"
BASE COUNT          678 a          595 c          608 g          636 t
ORIGIN      
        1 attgagagcc gttaactgcc tgttttatga gaaaaagtga tataactttt tattcattgc
       61 atagcagaaa atgtgatatt gcacgcacta tgtaataact tctcccactg acctggaaca
      121 actgaactta ttgaactatg ttagaaacta cgtcagttta agtatctgcg gaactgacaa
      181 ggttaagcac aatgatatat cggcgcgtat tccgttgcat aagtgtgcaa aaaaagtgga
      241 agacgtatcg agatttgtgc gtctgatcga gacatgttta aaaatggctt gccataatta
      301 acgttgtatg tgataacaga tttcgggtta aacgaggtac agttctgttt atgtgtggca
      361 ttttcagtaa agaagtcctg agtaaacacg ttgacgttga ataccgcttc tctgccgagc
      421 cttatattgg tgcctcatgc agtaatgtgt cagttttatc tatgttatgc ctgcgggcga
      481 agaaaacaat ctaaggaatt tttcaaatgg caaagattaa aggtcaggtt aagtggttca
      541 acgagtctaa aggttttggc ttcattactc ctgctgatgg cagcaaagac gtgttcgtac
      601 acttctccgc tatccagggt aatggcttca aaactctggc tgaaggtcag aacgttgagt
      661 tcgaaattca ggacggccag aaaggtccgg cagctgttaa cgtaacagct atctgatcga
      721 atccactgat ctgaagtgtg aatacgcttc aatctcgcta taaagcctcg tcgaatgcga
      781 ggctttttac tatgctttat cttcgctcct ggcgttcgga tatttgcccg ccgcgtgatt
      841 cgcgttacac ttgcggcctt tagtatcctg ccggagttgt catgtcgttt tcctgtccac
      901 tttgccatca gcctcttacg cgtgaaaaaa acagctatat ctgtccccaa cgacatcagt
      961 ttgatatggc gaaagaaggg tatgtcaatc tgctgcccgt tcagcataaa cggtctcgtg
     1021 atccgggcga cagcgcggaa atgatgcaag cacgccgcgc attcttagat gccggacatt
     1081 atcagccgct gcgtgatgca attgtcgccc aactgcggga acggcttaat gaaaaggcca
     1141 cggcggtgct ggatattggc tgtggtgaag ggtattacac acacgcattt gccgatgcgt
     1201 tgcccgaaat caccacgttt ggtctggatg tttcgaaggt agcgataaaa gcggcggcga
     1261 aacgctatcc gcaggtcact ctttgtgtcg cttccagcca ccgtttgccg ttttccgata
     1321 ccagtatgga cgccataata cgtatttacg cgccgtgtaa agcagaagaa ttagcacgag
     1381 tagtgaagcc cggcggctgg gtcattactg ccacgccggg accgcgacat ttgatggagc
     1441 tgaaggggct gatttacaat gaagtacatc ttcatgcacc tcatgcagaa caactggaag
     1501 gttttacatt acagaagagt gatgagttgt gttatccgat gcgtcttcgc ggtgatgaag
     1561 ccgtcgcatt attgcagatg acgccgtttg cctggcgtgc gaagccagaa gtctggcaaa
     1621 tactggcagc aaaagaagtg ttagactgcc agacggactt taatattcac ctctggcagc
     1681 gttcttatta accgtggaag tgcgtccaga ggatctggac gccgatgccg atcagcacca
     1741 gcccgccgag aatttccgct tttttcccaa taattgagcc gataaagcga ccaaccatca
     1801 tccctaatgt cgacataatc aaggttgcac aaccaatggc caatgcggtc gcgataatgt
     1861 tgacctgaag gaaagcaaga ccaacaccca cagccatggc atccaggctg gtggcaatcg
     1921 cggtggttac cagtagccag aaaccgtgcc gacggcgcgg ctcttcatct tcatcatctg
     1981 cgccacgaaa accctcaata atcattcgcc cgccgaggaa tatcagcagc acaaacgcaa
     2041 tccagtgatt ccattcaagg acaaaccggc tggctaacat gcccattccc cagccgatca
     2101 gcggcgtcag ggtttcgacg gcaccaaaaa taaggccggt tcgcaatgct tcagaaaatt
     2161 tcggtttatg gagggtggca cctttaccga ttgatgcagc aaatgcatcc atcgacatac
     2221 caaacgcaag aagaacagta gcagtgatat tcatgacaac gtcctgaccg gggtatccat
     2281 aaaacacatc atcgccccca gcaaacatgc atacatgaac aacaagccgc gatgggtttt
     2341 cgcgttcatg caggcatagc gattgatgtg catatggtct cgcctgactg aaagtcttca
     2401 gtccgtacgt gccacgtttt gcaacgagta tgttgacacg tacatttccg gaatctggaa
     2461 attggctact ccccaaatga gggcgcaacc ttaacatatt ttgagaatat aaaacaa
//
LOCUS       DBFIFM010000346         2510 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.348, whole genome shotgun sequence.
ACCESSION   DBFIFM010000346 DBFIFM010000000
VERSION     DBFIFM010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.348"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            158..2437
                     /gene="ydeP"
                     /locus_tag="N7C74_004754"
     CDS             158..2437
                     /gene="ydeP"
                     /locus_tag="N7C74_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HGU2434449.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAQNTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSVEFLARLGERYGFTPPHVPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
BASE COUNT          654 a          586 c          636 g          634 t
ORIGIN      
        1 atcgtattta atctattgtt taacgataat agattaaaaa tagaaagaga gtagtcccct
       61 actcttatat atccatgttg gcgataatcc ctttgtatgt actgtgcatc atcgctatta
      121 caaatcctaa taattcattt ccacacagga taagtagatg aagaaaaaaa ttgaatccta
      181 ccagggtgct gccggtggtt ggggtgctgt taaatccgta gcgaatgcag tacgtaagca
      241 gatggatata cgccaggatg ttattgccat gtttgacatg aataagccag agggctttga
      301 ctgtccgggt tgtgcatggc cagatcctaa gcacagtgcg tcattcgaca tttgtgaaaa
      361 cggcgcaaaa gcaatcgcct gggaagtcac ggataagcag gtaaatgcct ctttctttgc
      421 tcagaatacg gttcaatcat tacttacctg gggagatcac gagcttgagg ctgcgggacg
      481 tctcactcag cctttgaaat atgatgctgt cagcgactgt tacaagccat taagctggca
      541 acaagctttc gacgaaattg gcgcacgcct tcaaagctat agtgatccca atcaggttga
      601 attctatact tcgggccgca cttccaatga agctgccttt ctttatcagc tttttgcccg
      661 tgaatacggg agcaataact ttcccgactg ctccaacatg tgccatgaac cgacaagcgt
      721 gggtttggca gcgagtatcg gtgtaggtaa agggaccgtg ttgctggaag actttgagaa
      781 gtgcgattta gtcatttgca ttgggcataa ccctggtaca aaccaccctc gcatgctgac
      841 ttcgttgcgc gctttagtga aacggggagc gaaaatgatc gccatcaatc ctctacagga
      901 acgtggcctg gagcgattta ccgcaccgca aaacccgttt gaaatgctga cgaactctga
      961 gactcagttg gccagtgcct actataacgt gcgcattggt ggtgatatgg cgttgctcaa
     1021 ggggatgatg cgcctgttaa ttgagcgcga tgatgctgca agcgccgcag gtcggccctc
     1081 attgctggat gacgaattta ttcaaacgca taccgtcggc tttgacgagc tacgccgtga
     1141 cgtgctcaat tccgagtgga aagatatcga acgtatttct ggactaagtc agacacaaat
     1201 cgccgaactg gctgatgcat atgccgctgc cgaacgaacc attatctgtt acggaatggg
     1261 gatcactcag cacgaacatg gtacccagaa tgtacagcaa ctggtcaatc tgctgttgat
     1321 gaaaggtaac attggcaagc ctggtgcggg tatctgccca ctacgtggcc actctaatgt
     1381 acagggcgac cgaaccgtcg gtatcaccga gaaaccgtct gtagagtttc tggctcgcct
     1441 gggtgagcgc tatggcttca ccccacctca tgtacctgga catgctgcaa ttgccagcat
     1501 gcaagcaata tgtacggggc aggctcgagc attgatctgc atggggggca actttgcact
     1561 ggcaatgcca gatcgggaag cgagcgctgt accgttaacg caattagatt tggcggtaca
     1621 cgtagccact aagcttaacc gctctcatct gctgaccgca cggcatagct atattctgcc
     1681 ggttctggga cgtagcgaga ttgacatgca aaaaagcggt gcgcaggcgg taaccgttga
     1741 ggattcaatg tcgatgattc atgcctcgcg tggcgtgtta aaacccgccg gtgtaatgct
     1801 gaaatcagag tgtgctgtgg tcgcgggaat cgcgcaggca acactacccc agagcgtggt
     1861 agcctgggag tatctggtgg aagattatga tcgcattcgc aatgacattg aagctgtgct
     1921 gccagagttc gccgactata accaacgcat ccgtcatccc ggtggttttc acctgataaa
     1981 tgcagctgct gaaaggcgct ggatgacgcc gtcaggtaag gctaatttca ttaccagcaa
     2041 agggctgtta gaagatccct cttcagcgtt taacagtaag ctggtcatgg cgacagtacg
     2101 cagccacgat cagtacaaca cgacgattta tggtatggat gatcgctatc gaggggtatt
     2161 cggtcaacga gatgtggtct ttatgagtgc taaacaagct aaaatttgcc gtgtaaaaaa
     2221 cggcgaaaga gttaatctta ttgcgcttac gccagacggt aagcgcagct cacgccgcat
     2281 ggatagatta aaagtggtca tttaccctat ggctgaccgc tcactggtga cctattttcc
     2341 agaatcgaat cacatgctaa cacttgataa ccacgatcca ttaagtggca ttcctggcta
     2401 taaaagtatt ccggttgaat tagaaccatc aaattaatgc ctcttctcat ttcttctgct
     2461 gtcatccgca cagcagaaga attcctcatt gattattatc gcgcaatttg
//
LOCUS       DBFIFM010000347         2504 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.349, whole genome shotgun sequence.
ACCESSION   DBFIFM010000347 DBFIFM010000000
VERSION     DBFIFM010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.349"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..303
                     /locus_tag="N7C74_004755"
     CDS             <1..303
                     /locus_tag="N7C74_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU2434450.1"
                     /translation="DRWVVEQMSELTAMTASFVLATPTETDGALFPGRIMLANTCMWD
                     YRGDECGYHGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMVANFGGFLSINKLSQ"
     gene            complement(320..574)
                     /locus_tag="N7C74_004756"
     CDS             complement(320..574)
                     /locus_tag="N7C74_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HGU2434451.1"
                     /translation="MTKVRDIAPYSVRMPDSLKRDLTIRASKNGRSLNSEIVMILQAA
                     IDEEKSPRSIEGFAQQESEKFREALLKTLSSMYGEDKKPT"
     gene            684..794
                     /locus_tag="N7C74_004757"
     CDS             684..794
                     /locus_tag="N7C74_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HGU2434452.1"
                     /translation="MEKEVSRILVRIPQSLKDAITGKAKEECRSFNSEVM"
     gene            1097..1975
                     /locus_tag="N7C74_004758"
     CDS             1097..1975
                     /locus_tag="N7C74_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein/antirepressor Ant"
                     /protein_id="HGU2434453.1"
                     /translation="MKSIAKAQNDFTIFKFGDSEIRVINKCGEPWFVAKDVCDALALT
                     NSRKALTALDDDEKGVTLSYTLGGEQNLSIVSESGMYTLVLRCRDAVNKGSVPHKFRK
                     WVTAEVLPSIRKHGEYVKGKKTTVEERTPLRDAVNMLVGKKGLRNDDAYNMVHQRFGI
                     DSIDELSIEQIPLAVEYIHRVVLEGEFIGKQEKSTNELSAKEANSLVWLWDYANRSQA
                     LFRELYPALKQIQSNYSGRCYDYGHEFSYVIGMARDVLINHTRDVDINEPDGPTNLSA
                     WMRLKNKELPPSPHHY"
     gene            2029..>2504
                     /locus_tag="N7C74_004759"
     CDS             2029..>2504
                     /locus_tag="N7C74_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU2434454.1"
                     /translation="MLYSNILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPEDY
                     FRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGEIHKFR
                     CVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMAVTG"
BASE COUNT          682 a          526 c          665 g          631 t
ORIGIN      
        1 gaccgctggg tggtggagca gatgtcagag ctgacggcca tgacagcctc gtttgtgctg
       61 gcaacaccga cggagacgga cggagcgctg tttcccggtc gcatcatgct ggcgaacacc
      121 tgtatgtggg attaccgggg agatgaatgc ggttatcacg gtcctgcggt ggcggatgag
      181 ttcgacaacc ccaccacgga tatccgtaag gacagatgca gcaagtgcat gcgcgggtgt
      241 gagatgcgcg gcatggtggc taattttggc ggtttccttt ccattaataa actttcgcag
      301 taatggatta tgcccaccat caggtgggtt ttttatcttc gccgtacatg ctgctgagag
      361 tcttaagaag agcctcccta aatttttcag attcttgttg agcaaaacct tctattgatc
      421 ttggtgattt ttcttcatca atagcagctt gcaagatcat aactatttct gaatttaatg
      481 agcgtccgtt tttcgatgct cttatggtca ggtcgcgctt gaggctatct ggcattctta
      541 cgctatatgg ggctatgtct ctaaccttcg tcatacttac accgtgataa tcacattgat
      601 atcacagtgt attcaaaaaa actttgactt gatatagtca cagtgatacg tttgtgattc
      661 ataataactc acgcggtggc gatatggaaa aagaagttag cagaattttg gtgagaatac
      721 ctcagtcgct aaaagatgcg attacaggaa aggccaaaga agaatgccgg tcgtttaact
      781 cggaagtgat gtgattgctg gacagcctga agagagaggg gataacggta tgagtaaaga
      841 atgttgtttt ttgcggcatt agcgaatcag acgctgatca aacatacatt tactctaaag
      901 aaacaggtcg gatgctgtgt agtgactgcg tgtttgacat cataagatac aagtatcttg
      961 gatgttctgc cagcattagc aatataggtg aagtgtacga agggaaagat ataactgata
     1021 gagcagaaag ttgaagcccc aactgctgta acagtcaggg cttcgttatc aacaaatcgg
     1081 cttaggaaat attgacatga aaagtatagc aaaggcacaa aacgatttca ctatcttcaa
     1141 attcggcgac agtgaaatcc gcgtcatcaa caagtgcggt gagccgtggt ttgtagctaa
     1201 agatgtttgt gatgctttag ctttgactaa ctcacgcaag gcgcttactg cacttgatga
     1261 cgatgaaaag ggagtaactt taagttacac ccttggtggt gagcagaatc taagcattgt
     1321 gagcgaatca ggtatgtata cattggttct gcgctgccgc gatgcagtca ataaaggttc
     1381 agtcccgcac aaattccgca agtgggtaac agcagaagtt ctgccttcaa ttcgcaaaca
     1441 tggcgagtat gtaaaaggaa agaaaaccac tgttgaggaa agaacaccgc tacgcgatgc
     1501 agtaaacatg ctggtaggaa agaaaggact tcgcaatgac gatgcataca atatggttca
     1561 tcagcgtttt ggtattgaca gcattgatga gctttcaatt gaacaaatcc cgcttgccgt
     1621 agagtacatc cacagggtag tgcttgaagg tgaattcatt ggcaaacaag agaagagcac
     1681 caacgagctt tccgcaaaag aagcaaacag ccttgtatgg ctatgggatt atgccaaccg
     1741 ctcacaggca ttattccgcg aactgtatcc ggcgctaaaa caaattcaat cgaactattc
     1801 cggcagatgc tacgactacg gtcatgagtt ctcgtatgtt attggaatgg cgagagacgt
     1861 tttaattaat cacacacgag atgttgatat caatgagcca gacggaccaa cgaatctttc
     1921 cgcatggatg agacttaaga ataaagaatt acctccttca ccgcatcact actaacagat
     1981 tgccaacgaa atgacccagc ttcggctggg tttttatcag gagttctcat gctctatagc
     2041 aatatattgg cgcacgcccg gcgatgtgca ccagcggagt cgtgcggctt cgtggtgaga
     2101 acgccggaag gggaaagata ttttccctgc gtgaatatct ccggtgagcc ggaagattat
     2161 ttccggatgt cgccggagga ctggctgcgg gcagaaatgc agggggagat tgtggcactg
     2221 gtccacagcc accccggtgg tctgccctgg ctgagcgagg ccgaccggcg gctgcagata
     2281 aaaagtgcac tgtcctggtg gctggtctgc cggggggaaa ttcataaatt ccgctgtgtg
     2341 ccacatctga caggacggcg ctttgagcac ggggtgacgg actgttacac gctgttccgg
     2401 gatgcctacc atctggcggg aattgatatg ccggattttg agcgtgagga tgactggtgg
     2461 cgcaacggtc agaacctgta cctggacaat atggcggtca ccgg
//
LOCUS       DBFIFM010000348         2501 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.350, whole genome shotgun sequence.
ACCESSION   DBFIFM010000348 DBFIFM010000000
VERSION     DBFIFM010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.350"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..1284
                     /gene="espL1"
                     /locus_tag="N7C74_004760"
     CDS             <1..1284
                     /gene="espL1"
                     /locus_tag="N7C74_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HGU2434455.1"
                     /translation="VPLVRDAEFRALLRHPPAGSRVIASKDFGFALDIFFCRMMANNV
                     SHMSAILYIDNHTLSVRLRIKQSAYGQLNYVVSVYDPNDTNVAVRGTHRTARGFLSLD
                     KFISSGPDAQTWADRYVRNCAIAILPLLPEGVPGAIFTGIATRMPFAPIHPSAMLLIM
                     ATGQTQQLITLFRQLPILPEKEIIEIITAQNSIGTPALFLAMMNGHTDNVKIFMQEIQ
                     SLVDNHIIHEDNLVKLLQTKSANETPGLYISMLYGFDEIIDIFLNALTTPIAQELLNK
                     KLVMSILAMKIHDGEPGLYAAMENNHPLCVTRFLSKINGIAFKYKLSKANIMDLLKGA
                     TAQGTPALYIAMSKGNEDVVLSYISTLGAFAKKHSFSQHQLFTLLAAKNHDNMSAVHI
                     AIHHNHYKTVETYYAAINVISQSMSFSADELKTYL"
     gene            1459..1566
                     /gene="yniD"
                     /locus_tag="N7C74_004761"
     CDS             1459..1566
                     /gene="yniD"
                     /locus_tag="N7C74_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HGU2434456.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1619..2377)
                     /locus_tag="N7C74_004762"
     CDS             complement(1619..2377)
                     /locus_tag="N7C74_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HGU2434457.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          734 a          555 c          536 g          676 t
ORIGIN      
        1 gtccccttag tccgggacgc ggagttcaga gcattacttc gccatcctcc tgcagggagt
       61 cgcgttatcg cgagtaagga ttttggcttc gctttagata ttttcttctg tcgaatgatg
      121 gcaaacaatg tcagtcatat gtctgcgatt ttatatatag acaatcatac tttgtcagta
      181 aggctacgaa taaagcagtc ggcgtatggg caattaaatt atgttgtgtc cgtttacgac
      241 ccgaacgata ccaacgttgc cgtcagaggc acccacagga cagcacgggg ctttctctcg
      301 cttgataagt tcatcagttc aggtcccgat gctcagacct gggctgatag gtatgttcgc
      361 aactgtgcaa ttgctattct gcccctatta cctgagggag ttccaggggc tattttcacg
      421 ggtatcgcga cacgaatgcc atttgcccct atacatccat cggcaatgtt gttaataatg
      481 gccacaggcc agactcaaca gcttattaca ttattcagac agttgcccat actccctgaa
      541 aaagaaatca ttgaaataat aactgcgcag aatagcattg gtacacctgc tttatttttg
      601 gctatgatga acggacatac tgacaacgtg aaaatattta tgcaagaaat tcagtcactg
      661 gtagataatc acatcattca tgaagataat ctggttaaat tgttgcaaac taaaagtgct
      721 aacgaaacac ctggacttta tatctccatg ttgtatggat tcgatgagat aatcgatatc
      781 tttctgaatg cattaaccac tcctatagca caagagcttt taaacaaaaa actggtgatg
      841 agtattttag ccatgaaaat acatgatggt gagccaggat tatacgccgc aatggaaaat
      901 aatcaccctt tgtgtgtcac acggttcctc tctaaaatta atggcatcgc ctttaaatac
      961 aagttgagca aagctaacat catggattta ttaaaaggcg ctacagcaca gggaacccct
     1021 gcattataca tcgctatgag caagggtaat gaagacgtcg tgttatctta tatatcgacg
     1081 ctgggtgctt ttgcaaaaaa acattctttt agtcaacatc agttatttac actgttggct
     1141 gctaaaaatc atgacaacat gtcagctgtt catatagcca ttcatcataa tcattataaa
     1201 actgtagaaa catattatgc tgctataaat gtaatcagcc aaagcatgag ttttagtgct
     1261 gatgaattaa agacgtattt ataaccagct atttacgata tttatatatc gatacataat
     1321 tttatttttt cataaaacgc agaaacagtg atcaggtgta caacgccacc cgacggctgt
     1381 aataacaaaa aatcccttga tgcctgtccc ttttgttaca ctccgttatc acgcacaaga
     1441 gatatgcagg acactggtat gccgactaaa cgctttgata aaaaacactg gaagatggtg
     1501 gtggtgctac tggcaatctg tggcgctatg ttgttgctac gttgggcagc aatgatttgg
     1561 ggctgagcag tgacaaacga agacagccag accgcataac acggtccggc agataatttt
     1621 acatgctgta gcccagcgat aaggttgtcc gacggtcggt atgttccggt gcagactctg
     1681 gaggttcaga gttccatgtg acgttgtaag ccaccttcag tccaaaatgc tcattaatag
     1741 caacatttaa tgcgctttcg gagttcagcg ttgtgtcttc cgcgccaaag acggaaacac
     1801 cctgcgtaaa tttagcgttg tcagtaagct gccatgcata agcaccggag gcatagccca
     1861 gcggctgagt ttcactggca ttgtcggtat atttgtcgta acgcacacct ggaccgaatt
     1921 caaagcggaa actgtgtacc gggccattca aaaactgacg accataaccc gcggtcaaca
     1981 catcgcgctc tcgatagccg ttataacggt ctgtcagcca gcttgcctga ccaaataaat
     2041 agtcataatc agttaaatta aaacggctac gcccgcccgc cgcatatttt tctgaagaac
     2101 gctcatcatt agaagaagta ttactggcgt tcccccacag cgaccaggca gtggtttgtc
     2161 cataccaggt catggtggtg tcagccgtaa gtgaggagct tttcgtattg cctgattgtg
     2221 caagataccc tgcgttcaga ttaccttcga aaggtttttt agcgctggca ggatcgtcca
     2281 tgacagtaaa aacggaatca tcggcagctg cattcagtga cgcaaacatg ccccccgcca
     2341 acataacgat ggcaggaact gtcttcaaaa gcttcattta tcaagagtcc gtacaacaaa
     2401 aaaagagacc atcgcggtcc cggaaacttt cttaaggatc aaagattagc gtccctggaa
     2461 aggtaacgaa ttataaaaag gcgcgaataa cttatcaatg t
//
LOCUS       DBFIFM010000349         2481 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.351, whole genome shotgun sequence.
ACCESSION   DBFIFM010000349 DBFIFM010000000
VERSION     DBFIFM010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.351"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            96..2363
                     /gene="adiA"
                     /locus_tag="N7C74_004763"
     CDS             96..2363
                     /gene="adiA"
                     /locus_tag="N7C74_004763"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HGU2434458.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFAADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT          624 a          673 c          614 g          570 t
ORIGIN      
        1 ttcaaaaatg tttgtttttc acgcgcttta cagcccgaaa aggccggaag atacttgccc
       61 gcaacgaaga ttccttcata accgggtaag caatgatgaa agtattaatt gttgaaagcg
      121 agtttctcca tcaagacacc tgggtcggta acgctgttga gcgtctggca gatgctttaa
      181 gccagcaaaa tgttaccgtg attaaatcca cctcctttga tgatggtttt gccattctct
      241 cttcaaacga agccattgac tgcctgatgt tcagctatca aatggaacat ccggacgaac
      301 atcaaaacgt cagacaattg atcggtaagc ttcatgagcg ccaacaaaac gtgccggtct
      361 tcctgttggg cgatcgggaa aaagccctcg ccgcaatgga ccgggatctg ctggagcttg
      421 tcgatgaatt cgcctggatt ctggaagata ccgccgactt tatcgccgga cgcgccgttg
      481 ccgcgatgac ccgctaccgc cagcagctgt tgccgccact gttcagcgcg ctgatgaaat
      541 atagcgacat ccatgaatat tcctgggcag cgccaggcca ccagggcggc gttggtttta
      601 ccaaaacacc cgccggacgt ttctaccatg actactacgg tgaaaatctg ttccgcaccg
      661 acatgggcat cgaacgaact tccctcggtt ctttgcttga ccatactggc gcatttggcg
      721 aaagcgaaaa atatgccgca cgcgtatttg gtgccgatcg ctcctggtcg gtagtcgtcg
      781 gtacttccgg ctctaaccgc accatcatgc aggcttgcat gaccgataac gatgtcgtgg
      841 tcgttgaccg taactgccat aaatccatcg aacaaggttt gatgctgaca ggcgcgaaac
      901 cggtctacat ggtgccaagc cgcaaccgct acggcattat cgggccaatc tatccgcagg
      961 aaatgcaacc tgaaaccttg cagaagaaaa tcagtgaaag cccgctgact aaagacaaag
     1021 ccgggcaaaa accgtcttac tgcgtggtga ccaactgcac ctatgacggc gtgtgttata
     1081 acgctaaaga agcgcaggat ctgctggaaa aaacctccga tcgtctgcac tttgacgaag
     1141 cctggtacgg ctatgcacgt ttcaacccga tctatgccga tcactatgcc atgcgcggcg
     1201 aacctggcga tcacaacggt cctaccgttt tcgccaccca ctccacccac aaactgctga
     1261 atgcgctttc acaggcctct tatattcatg tacgtgaagg tcgtggggcg attaacttct
     1321 cccgcttcaa ccaggcctac atgatgcatg ccaccacctc cccgctgtat gccatctgcg
     1381 catccaacga cgtggcggtg tcgatgatgg acggcaacag cggcctgtca ctgacgcagg
     1441 aagtgatcga cgaagcggtt gatttccgtc aggcgatggc gcggctgtat aaagagttcg
     1501 ccgctgacgg tagctggttc ttcaaaccgt ggaacaaaga agtcgtcacc gacccacaaa
     1561 ccggcaaaac ctatgacttt gctgacgcac caaccaaact gctgaccacc gttcaggact
     1621 gctgggtaat gcatccgggc gaaagctggc acggcttcaa agatattccg gataactgga
     1681 gtatgctcga cccgattaaa gtcagcatcc ttgctccggg aatgggtgaa gatggtgaac
     1741 tggaagaaac cggtgttccg gcggcgctgg tcactgcctg gcttggtcgc cacggcattg
     1801 tgcctacccg caccactgac ttccaaatta tgttcctgtt ctctatgggc gtaacccgtg
     1861 ggaaatgggg gactctggtt aacacccttt gctccttcaa acgccactat gacgccaaca
     1921 caccgctggc gcaggtgatg ccggaacttg ttgaacaata tcctgacact tacgcgaaca
     1981 tggggattca cgatctgggt gacaccatgt ttgcctggct gaaagaaaac aaccctggcg
     2041 cacggttgaa cgaagcctat tccggcctgc cggtggcgga aatcaccccg cgtgaagcgt
     2101 acaacgcgat tgtcgacaac aatgtcgaac tggtatccat tgaaaatctg ccaggacgta
     2161 tcgcggcaaa ctcagttatc ccgtatccgc caggaatccc gatgctgctg tctggtgaaa
     2221 acttcggcga taaaaacagt ccgcaagtaa gttatttacg ctcgctgcaa tcctgggacc
     2281 atcatttccc tggatttgaa cacgaaactg aagggactga aattattgac ggtatttacc
     2341 acgttatgtg tgtgaaagcg taaccactat tccgctgaag gcgtaattgt ttaaataaca
     2401 ttacgccgcc tggccttagg ccttttgagt atggcaacgt tttcataaaa attgctgcaa
     2461 acaaaaatgt catacttttt g
//
LOCUS       DBFIFM010000350         2468 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.352, whole genome shotgun sequence.
ACCESSION   DBFIFM010000350 DBFIFM010000000
VERSION     DBFIFM010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.352"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..2313)
                     /locus_tag="N7C74_004764"
     CDS             complement(<1..2313)
                     /locus_tag="N7C74_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4851/ECs_5259 family protein"
                     /protein_id="HGU2434459.1"
                     /translation="MLVKTPVNPLLQWLNMFFSRRSLSGADGRALYAYRCTDTEYESL
                     AELLRTYAPRSYPRTIFISYSDVLFSIYAAEFIRRTHTVGHPKWDTILDSINWKVPYV
                     HRQKLVNDGIRYWKRKIRNLGQASGYLHTLACEGGLPIRMIENESGYLITYFRRIYQA
                     LRGQSSQYPAAKIAQELGDTIPVTMQNELVYEIAGEFCETLCRLLSEHPPHSSDPVSA
                     LRKLSPDWHLQLPLVLPEANAAEIVRRLLSQSSEIRSASSLQVERIWVDVDDSWYCDA
                     RFRFPATMRTEQLTSLFECHIQPEQTRLIISGKWKNGGARLAMLSRYEQQDWRVELLP
                     IAMQKLSGADAMAEISLSLHEGPILLGHTIPKGGYELTEELPWVFEAMNESESQLKLV
                     GMGSVSSRLNALFISLPKNSHLDISGEGEFDIPRLLKNSERSLTKISGVFSVVLHDGA
                     VCTIRTQQLYDSAIEYYIKSTEVELVKSDYPVHRAWPKIGWKKDLQYGIVPEKELFWR
                     SIRSGNNAWYSVASEMPKGQIEVRRIVNDEVLFSGKVVVLPADFDINIIPESAQQGII
                     MLSGITDTRIDKYSNNEKVTLKSDYSQNECAIYYNSSLMLENTVDLRVSWKDGSNLKL
                     LLPKPVSGGRFVTNDGSVHFDGVASIAHLHGIDAELLTISCAGRGYLNIELLDENPVA
                     EKFRYLHADLPLLSGRNDKLQQISLYENYNLLNAMLACAWNSNSTLCVDFYSDRFGKD
                     KATLNIKRYDGSFIEHDQGLLVDIKNSVVFP"
BASE COUNT          757 a          547 c          454 g          710 t
ORIGIN      
        1 aggaaaaaca acagaatttt ttatatcaac cagtaaccct tgatcgtgtt caataaaact
       61 accatcgtag cgtttaatat tgagtgttgc tttatccttt ccaaatctgt cagagtaaaa
      121 atcaacacat aatgtactat tgctgttcca tgcacatgcc agcatagcat ttagcaaatt
      181 atagttctca taaagtgaaa tttgttgtaa tttgtcattg cgtcctgata aaagaggaag
      241 gtcggcatgt aaatagcgga atttttcagc tactggattt tcatccaata actcgatatt
      301 aagatatcct cttccagcac atgatatggt taataactca gcatctattc catgcaaatg
      361 tgctatagat gccacaccat caaaatgaac agaaccatca ttagttacaa atcggccacc
      421 gctaaccggc ttaggtaata atagtttaag attagaacca tctttccagg agacccgtaa
      481 atcaacggta ttttccagca tcagcgatga attataataa atagcacact cgttttgtga
      541 ataatcggac ttaagtgtta ctttttcatt gtttgaatat ttatcaattc tagtatccgt
      601 tataccagaa agcataataa tgccttgctg agcactctca ggtataatat taatatcgaa
      661 gtctgctggt aaaactacaa ctttaccgct aaataaaacc tcatcattaa ctattcgccg
      721 gacttctatc tgtccttttg gcatttctga tgcgacagaa taccaggcat tattacctga
      781 acgaatggaa cgccaaaaaa gttctttctc cggtacaatg ccatattgca gatccttttt
      841 ccaaccgatc ttcggccagg ctcgatggac aggataatcc gatttaacca gttcaacttc
      901 tgtcgattta atataatatt caatcgcaga atcataaagt tgctgggtgc gaattgtaca
      961 aacagcacca tcatgtaaaa caacgctaaa tactccactt attttagtta agctgcgttc
     1021 actgtttttc agcaaccttg ggatatcaaa ctcaccttct ccactaatat ctaaatggct
     1081 atttttcggt agcgaaataa acagagcatt tagcctggaa ctcacagacc ccataccaac
     1141 aagttttagc tgcgattcac tctcattcat cgcttcaaaa acccagggaa gttcttcagt
     1201 aagttcataa ccgccttttg gaattgtgtg acctaacaga attggacctt catgaagcga
     1261 caatgagatt tcggccattg catctgcacc agagagtttt tgcatcgcaa taggtaataa
     1321 ctcgacccgc caatcttgct gctcataacg gcttagcatt gccaaccttg ccccgccatt
     1381 tttccatttt cctgaaatga tgagtcgggt ctgctccggc tgaatatggc attcaaacaa
     1441 agaggttaac tgttctgtac gcattgtggc cgggaatcgg aaccgggcat cgcaatacca
     1501 actgtcatca acatcgaccc agattctttc aacctgaaga ctacttgcac tgcgtatttc
     1561 agaagattga gaaagtaatc gccttacaat ctctgcagca ttcgcttcgg ggagaacgag
     1621 tggaagttga agatgccagt ctggagagag cttacgtaat gcagaaacgg gatcactgct
     1681 atgaggagga tgctcactaa gtaatcggca gagagtctcg cagaattcac cggctatttc
     1741 atagaccagt tcgttttgca ttgtgacagg aatcgtatcg cctaattctt gtgcaatttt
     1801 agcggcagga tattgagatg attgcccacg aagcgcctga tatatccttc tgaaataggt
     1861 aatcaaataa ccgctctcat tttcaatcat gcggataggc aaaccacctt cacaagccag
     1921 tgtatgcaga taacccgaag cttgtcccag gtttcttatc tttcttttcc agtagcgaat
     1981 gccatcatta accagcttct gtcgatgaac atacggaact ttccagttaa tagaatctaa
     2041 aattgtgtcc catttagggt gtccgaccgt atgagtccgg cggataaact ctgcggcata
     2101 tatgctaaat agaacatcgc tataggaaat gaatatcgtt cttggataac tacggggggc
     2161 atatgtacgc agtagttctg ccaaactttc gtactctgta tcagtacaac ggtaagcata
     2221 taacgcccgt ccatcagctc cggataatga acggcgacta aaaaacatgt taagccattg
     2281 caaaagtggg ttaactggag ttttcacaag caacctgcct tcctttgata caattcgtaa
     2341 caggttacta tcatcataaa aaagctcaac ccgatgaact cgctaaaaat gagacaaatc
     2401 atttatatct cgaaaaaact tgttacaatc atgagcgcta caccgaactt aaccatataa
     2461 attatgtg
//
LOCUS       DBFIFM010000351         2426 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.353, whole genome shotgun sequence.
ACCESSION   DBFIFM010000351 DBFIFM010000000
VERSION     DBFIFM010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.353"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..2200
                     /locus_tag="N7C74_004765"
     CDS             <1..2200
                     /locus_tag="N7C74_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU2434460.1"
                     /translation="DRLVQQRGFDGRTQRYHYDLTGKLTQSEDEGLVTLWHYDESDRL
                     THRTVNGEPAEQWQYDEHGWLTEISHLSEGHQVAVHYGYDDKGRLAGERQTVHNPETG
                     ELLWQHETEHAYNEQGLANRVTPDSLPRVEWLTYGSGYLAGMKLGGTPLVEFTRDRLH
                     RETVRSFGNNAYELTSTYTPAGHLQSQRLNSQVYDRDYDWNDNGDLVRISGPRQTWEY
                     GYSATGRLESVRTLASDLDIRIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHY
                     VYRHDEYGRLTEKTDRIPAGVIRTDDERTHHYHYDSQHRLVFYTRIQHGEPLVESRYL
                     YDPLGRRMAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTVQTDTTRIQTVYEPG
                     SFTPLIRVETENGEREKAQRRSLAETLQQEGSENGHGVVFPAELVRLLDRLEEEIRAD
                     RVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHFYHCDHRGLPLALISEDGNTAWR
                     GEYDEWGNQLNEENPYYLHQPYRLPGQQHDEESGLYYNRNRYYDPLQGRYITQDPIGL
                     AGGWNLYNYPLNPIIRMDPLGLYNLYQLLYDVWHDDSYGTSSIDITGSGDLISLGGHA
                     GLGVAFAKKKGEMLSDICIYATACGHAGIGGGINAAITYSETKSLPTSGVSNSVGVTV
                     GGGVGGHFAYTYVVDVDNPESSTESVGIGAGVDASVMTLACRTWQECWVN"
     gene            2212..2397
                     /locus_tag="N7C74_004766"
     CDS             2212..2397
                     /locus_tag="N7C74_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434461.1"
                     /translation="MKKYIFLVLFLLYGCSPVDIEPARQEVNIFRGLYQTGKYSDIYK
                     ITSSDFQTATSEKNLST"
BASE COUNT          617 a          584 c          758 g          467 t
ORIGIN      
        1 tgaccggctg gtacagcagc gcggctttga cgggcggacg caacgttacc actatgacct
       61 gaccggaaaa ctcacgcaga gtgaagatga ggggcttgtc accctctggc actacgacga
      121 atcggaccgc ctcactcacc gcacggtgaa cggcgaaccg gcagagcagt ggcagtacga
      181 cgagcacggc tggctgacag aaatcagcca cctgagcgaa ggccatcagg tggcggtgca
      241 ttacggttat gatgataagg gccgcctggc cggggagcgc cagacggtgc ataacccgga
      301 gacgggggaa ctgctgtggc agcatgagac agagcacgca tacaacgaac agggtctggc
      361 aaaccgcgtc acgccggaca gcctgccgcg ggtggagtgg ctgacctacg gcagcggtta
      421 tcttgcgggg atgaagctgg gcgggacgcc gctggtggag ttcacgcgcg acaggctgca
      481 ccgcgagacg gtgcgcagct tcggcaataa cgcatacgaa ctgaccagca catacactcc
      541 cgcaggccat ttacagagcc agcgcctgaa cagccaggtg tatgaccgtg actacgactg
      601 gaatgacaat ggcgacctgg tgcgcatcag cggcccgcga cagacgtggg aatatggcta
      661 cagtgccacg ggcaggctgg agagcgtgcg cacccttgca tcagacctgg atatccgcat
      721 cccgtatgcg accgacccgg cgggaaaccg gctgccggac ccggagctac acccggacag
      781 cacgctcacg gcgtggccgg ataaccgcat cgcggaggat gcgcactatg tctaccgaca
      841 cgatgaatac ggcaggctga cggagaagac ggaccgcatc ccggcgggtg tgatacggac
      901 ggacgacgag cggacccacc actaccacta cgacagccag caccgcctgg tgttctacac
      961 gcggatacag catggcgagc cactggtcga gagccgctac ctctacgacc cgctgggacg
     1021 gcgaatggca aaacgggtct ggcggcggga gcgtgacctg acggggtgga tgtcgctgtc
     1081 gcgtaaaccg gaggtgacgt ggtatggctg ggacggagac aggctgacga cggtgcagac
     1141 tgacaccaca cgtatccaga cggtatacga gccgggaagc ttcacgccgc tcatccgggt
     1201 cgagacagag aacggcgagc gggaaaaagc gcagcggcgc agcctggcag agacgctcca
     1261 gcaggaaggg agtgagaacg gccacggcgt ggtgttcccg gctgaactgg tgcggctgct
     1321 ggacaggctg gaggaagaaa tccgggcaga ccgcgtgagc agtgaaagcc gggcgtggct
     1381 tgcgcagtgc gggctgacgg tggagcaact ggccagacag gtggagccgg aatacacacc
     1441 ggcgcgaaaa gttcattttt accactgcga ccaccggggc ctgccgctgg cgctcatcag
     1501 cgaagacggc aatacggcgt ggcgcgggga gtatgatgaa tggggcaacc agcttaatga
     1561 agagaacccg tattacctgc accagccata ccgtctgccg gggcagcagc atgatgagga
     1621 atcagggctg tactataacc ggaaccggta ctatgacccg ctacagggga ggtatattac
     1681 acaagacccc attgggctgg cggggggatg gaatctgtat aattacccac tgaatccgat
     1741 aataaggatg gatcctttgg gtttgtataa tttatatcaa ttattatatg atgtttggca
     1801 tgatgattca tatggaacat catcaattga tattactggc agtggagatc taatatcatt
     1861 aggtggtcat gcaggacttg gcgttgcgtt tgctaaaaag aaaggtgaaa tgttatctga
     1921 tatttgtatt tatgctacag catgcggaca tgcaggaatt ggtggtggga taaatgcggc
     1981 tatcacatat tctgagacca agtctttacc tacatcggga gtcagcaatt cagtaggtgt
     2041 aacggttggc ggcggagttg gggggcattt tgcgtatact tatgtagtgg atgttgataa
     2101 tccagaatca tcgacagaat ctgttggtat cggtgcaggt gttgacgctt cagttatgac
     2161 tctggcttgt agaacgtggc aagaatgctg ggtcaattaa caggaggaaa tatgaaaaaa
     2221 tatatatttt tagttttatt tttattgtat ggttgttctc cagtggacat agagccagct
     2281 agacaagaag ttaatatctt tcgtggttta tatcagacag ggaaatacag tgatatatat
     2341 aaaatcactt caagtgattt tcagactgcc acatctgaaa aaaatttatc gacataatga
     2401 acgaagcaaa agaaaaagat cttggg
//
LOCUS       DBFIFM010000352         2417 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.354, whole genome shotgun sequence.
ACCESSION   DBFIFM010000352 DBFIFM010000000
VERSION     DBFIFM010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.354"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(141..1145)
                     /gene="argF"
                     /locus_tag="N7C74_004767"
     CDS             complement(141..1145)
                     /gene="argF"
                     /locus_tag="N7C74_004767"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HGU2434462.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKPFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1307..1723
                     /gene="rraB"
                     /locus_tag="N7C74_004768"
     CDS             1307..1723
                     /gene="rraB"
                     /locus_tag="N7C74_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HGU2434463.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1769..2272)
                     /locus_tag="N7C74_004769"
     CDS             complement(1769..2272)
                     /locus_tag="N7C74_004769"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU2434464.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALKAMEEAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
BASE COUNT          633 a          593 c          563 g          628 t
ORIGIN      
        1 cgtttaaata cactaaatcc tccggaaaat ttacgtatag caatagaaaa atttggctgg
       61 aagaaaaaaa ctatcactgc ataaaataat ttgacgccgg gatgtttctg tatttcccgg
      121 catctttata gcgatatcaa ttatttactg agtgtcgcga ccatcaccgc tttgatggtg
      181 tgcatgcggt tttccgcctg atcaaaaaca atgctggcgg cagattcgaa gacctcatca
      241 gtcacttcca ttccgccatg taggccaaac tcttccgcca ttttcttgcc aagcgtcgtt
      301 tggtcatcat ggaacgcggg caggcagtgg aggaatttga cctccgggtt accggtcaac
      361 tgcatcatct tgctgttcac ctgataatca cgcagtaatg caatgcgttc agcccatttt
      421 tcttttgctt cacccatcga cacccataca tcggtataga taaagtcagc accttcaacg
      481 cctttagcta catcttccgt cagcgtaata ttcccgccat tttgttgtgc cagggcgcgg
      541 cattccgtaa ccagcgcagc ttccggccag catgcctgtg gcgcgaccag acgcaaatcc
      601 agaccggtaa gcgccgcagc ttcgagcatc gaattgccca tgttgttacg tgcgtcacct
      661 gcatagacca gcgtcatttc gttgaatggt ttaccgggta aatgctcctg catggtgagg
      721 agatccgcca gcaactgcgt ggggtgaaac tcattggtca ggccgttcca taccggcacg
      781 ccagcgtatt ccgccagtgt ttcgacaatc tcctgaccat agccgcgata ctgaataccg
      841 tcatacatgc gacccagcac gcgggcagtg tctttaatcg actctttatg accaatctga
      901 ctgccgcttg ggccgagata agtaacgcga gcaccctggt catatgcggc aacttcgaaa
      961 gagcatcggg tacgggtcga gtctttttcg aagatgagcg cgatgttttt accagtgagt
     1021 ttggcttctt ctttgccgct tttcttatcg gctttcagct tcgcggctaa ctgcagcagg
     1081 ctgttgagtt cagctggcgt gaaatcgagt aatttcagga aatgcttatg ataaaacccg
     1141 gacatagatc cctcctgtgg ctaacgtctc aatgaattaa aattcaattt atatggatga
     1201 ttattcattt gcaagtctaa agcataaatc tttgtcacaa aggtggaggc aatgccagtg
     1261 gtgtgtgaca atatgagtat cggcaggaca ttaagaggaa tgagccatgg caaacccgga
     1321 acaactggaa gaacagcgtg aagaaacacg tttgattatt gaagaattac tggaagatgg
     1381 cagcgatccg gacgcgctgt acaccatcga acaccatctt tccgcagacg atctggaaac
     1441 cctggaaaaa gcagcagttg aagcgtttaa actcggttac gaagtgaccg atccagaaga
     1501 gctggaagtt gaagatggtg atatcgtgat ttgctgcgac atcctcagcg agtgcgcgtt
     1561 gaatgccgat ctgatcgatg cccaggttga acaactgatg acgctggcgg agaaatttga
     1621 cgttgagtac gacggttggg gcacttactt tgaagatccc aacggcgaag acggcgacga
     1681 tgaagatttt gtcgatgaag acgatgacgg ggttcgccac taattaattg attaaaatgc
     1741 ccgatagcac acactagcgg gcattttttt agagttcgcg tagcatccgc acttcacaat
     1801 caacatggcc cgtgcagcca agtgcatagt cgatatgttg aaaacctaaa tgttcataca
     1861 aaccaatcgc ttctttcaaa aaagcggtcg tttccagata gcagcgtttg aagcccattt
     1921 ctcttgcctc ctccatcgcc tttaaggcca gtttttttgc cagccctttg ccgcgaatag
     1981 ccggaagaaa atacatcttt tgcagttcgc aaatgtccga ttcactacct gctaatggcg
     2041 caatcccacc gccgccgacc acttcaccct cgtactcaac cacccaatat gcatgacccg
     2101 gctgactata cacttgatac agctcgtcaa gattcggatc ggcgacggtg tagcctttat
     2161 cagcggtaag accgtattcg gcggatacct gacgaatgac gcgggcgata gcaggattat
     2221 cctgcagcgt caggcgacgc attacaggtg attgcggcgc aatgttattc atagtttaac
     2281 ccaataaaaa taaagtaatt atacatttgt ttaataccac tctcttctta cgtgcgcaag
     2341 cgtcttataa agcaataaag tttttgccag cctattactg ataagagtat tgtaacgtgg
     2401 cagaacattt tactgtc
//
LOCUS       DBFIFM010000353         2403 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.355, whole genome shotgun sequence.
ACCESSION   DBFIFM010000353 DBFIFM010000000
VERSION     DBFIFM010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.355"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..720
                     /gene="yahL"
                     /locus_tag="N7C74_004770"
     CDS             <1..720
                     /gene="yahL"
                     /locus_tag="N7C74_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HGU2434465.1"
                     /translation="KSSLVSCVDIFKVLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRD
                     CEWSDDNALTINVQFNDFPGFYDWMDYPDHPVKFVFRILENQKGTVWVYDQDDAFLDI
                     KANVQAGRFTGLKKLVQFINSVRTDCKCILLEHHMPLLRLFPKGKECMHVEKWLREMS
                     SIPETDAPIKQALAHGLLLHLKNIYPVFPESLVMLLLSVLDVKTYRDDARLNEGISNR
                     VQELGDRYYPVNKHVKIRYTL"
     gene            1172..1381
                     /locus_tag="N7C74_004771"
     CDS             1172..1381
                     /locus_tag="N7C74_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434466.1"
                     /translation="MPLLSSLQSGTLPLFGYSGRVRPMKKAHTGESGLKWEAKDSSKL
                     IGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(1398..2069)
                     /gene="yahN"
                     /locus_tag="N7C74_004772"
     CDS             complement(1398..2069)
                     /gene="yahN"
                     /locus_tag="N7C74_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HGU2434467.1"
                     /translation="MMQLVHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPINAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            2216..>2403
                     /gene="yahO"
                     /locus_tag="N7C74_004773"
     CDS             2216..>2403
                     /gene="yahO"
                     /locus_tag="N7C74_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HGU2434468.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKA"
BASE COUNT          732 a          499 c          534 g          638 t
ORIGIN      
        1 aaaagctccc tggtttcctg tgtcgatata ttcaaggttt tgattaaaca atattatgat
       61 tatccttatg attgtcgtga tgatttagtc gatgacgaca aactcattca tctcatggct
      121 gccgtacgag actgtgagtg gtcagacgat aacgcactca ccataaatgt gcagtttaat
      181 gatttccctg gattttatga ctggatggat taccctgatc acccggttaa gtttgttttt
      241 cgcatacttg aaaatcaaaa agggacagtt tgggtatatg accaggatga tgcgtttctt
      301 gatattaaag caaatgtgca ggctggacgc tttaccgggc ttaagaaatt agtgcagttt
      361 atcaattcag tgcgaacaga ttgcaaatgt attcttcttg aacaccatat gcctttgctg
      421 agacttttcc ctaaagggaa agagtgtatg catgtggaaa aatggcttcg cgaaatgtcg
      481 tctattccgg agacagacgc gcccattaag caggcgcttg cgcatggatt attattacac
      541 ttaaaaaata tctaccctgt ttttcctgaa tcactggtga tgttattgct ctctgttctg
      601 gacgtaaaaa catacagaga tgatgcacga ttaaatgaag ggatatctaa tagagtccag
      661 gaattaggtg acagatatta tcctgtaaat aagcacgtta aaatccgcta cactctttag
      721 ttcagaaagg gttccacctg caagttgtta ttctacctga gagtgaatcg caatgcaggt
      781 aatcatttca tctgtatatc tgtatgccaa ctggcattcc atctcttttt tcgccgatat
      841 tttgttgtga tcggcgacac ttcgcttaaa aaagcaccag tagtggtttc gcagccatgc
      901 ggtgtataaa aaaaaatgat ctcatgcaga tgttttgtga atgtgtgggt tgacattcat
      961 atgaaaaaaa tcataattcc atcatgtttg tgtggggtct tttctgtatc ttacgcatcg
     1021 cactcaagcc tgacagaaaa tatgctgtaa ggctcatatc aaaaaccgcc attagctcat
     1081 caggaagagc agacgacaac caggattgtt gtatggtacg gggttcgagg ccccgatggc
     1141 ggtccaactt ttcaaaactc ttctgaatta gatcccctta ttatcatccc tgcaaagtgg
     1201 aacattgcca ttgtttggct attcaggacg ggtcagacca atgaaaaaag cccacacagg
     1261 ggagagtggg ctgaaatggg aagctaaaga ctcaagtaaa cttatcggaa ataaggacca
     1321 cgcattacgg gggctatcat cgccgatggt cgggattcgg caaatcaggt tgattacgta
     1381 gagagtaaat tatctgctca ccgctgcgtc accccttcgt aaatcaggcg tagcgcgaat
     1441 acaccaataa ttgcaccaat aacccgactg gcaacgcgtt gcatacgccc ataagcacga
     1501 cgcacagcgg gcaaagaaaa cgcctgacta agaaaaactc gccagataat tgatgcgagc
     1561 acaatccccg cccaggccat taaacgtgcc catgttggtg tttcggcatt taatgttact
     1621 gagaaaatac taataaaaaa taaaacggtt tgtgggttag agagatcggt aattaatcca
     1681 cggcgaaaaa agacatacca gggggcgtta atcggttgtt gtagtgtgct catttgcggt
     1741 gttgactggc ggcgcatgct gcaccacgca aaccataaga gataagcgcc gccgacgatt
     1801 ctgataagcg aaaaaatctc ctcacactgc gtaattagcg ttgcaagacc aaacaaaccc
     1861 aacccggaat aaaatgcatc gcccagcgcc acgcccagtc cggtcagcac tcctgcgcgt
     1921 cgaccggaag ccaggctggt ttgtactacc acaaaaagat tggctcccgg attaaaaaaa
     1981 gtaatcacga acagtcctac ggtcaggtaa acggcatgca aaggatccat agtgatttca
     2041 tccataaata agtgaactaa ctgcatcata tttctaccaa aaataatcgg gtgcgagaga
     2101 gatcacaaag tgtcttattt ccggttactg gcgtttatgc cctgactgaa ctaattatta
     2161 atcaacccaa taatgtcggt gggtgatagt gtgataacaa ctctggagcc gtaatatgaa
     2221 aataatctct aaaatgttag tcggtgcgtt agcgtttgcc gttaccaatg tctatgccgc
     2281 tgaattgatg accaaagcgg aatttgaaaa agttgaatcg cagtatgaaa aaataggtga
     2341 tatttcaacc agtaatgaaa tgtcgactgc agatgcaaaa gaagatttaa tcaaaaaagc
     2401 cga
//
LOCUS       DBFIFM010000354         2332 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.356, whole genome shotgun sequence.
ACCESSION   DBFIFM010000354 DBFIFM010000000
VERSION     DBFIFM010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.356"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            217..914
                     /locus_tag="N7C74_004774"
     CDS             join(217..466,466..914)
                     /locus_tag="N7C74_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
                     /protein_id="HGU2434469.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSPCRKTWQLQFT
                     YTASQPGKHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEV
                     VVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHYQ"
     gene            931..1191
                     /locus_tag="N7C74_004775"
                     /pseudo
     CDS             931..1191
                     /locus_tag="N7C74_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
     gene            complement(1333..>2332)
                     /locus_tag="N7C74_004776"
     CDS             complement(1333..>2332)
                     /locus_tag="N7C74_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HGU2434470.1"
                     /translation="NKAAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSD
                     EMADNLIDFAGKWRPDLIVYPPLDPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRS
                     LSNAYRRHGVSGPLCDLAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRD
                     SDRKRLLISLGTVKPMVDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRL
                     VDWIPMGVFLNGADGFIHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGI
                     IPDKHGLTSDLVNRLLYDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
BASE COUNT          660 a          536 c          491 g          645 t
ORIGIN      
        1 aggaaaataa acgttcattc tatggttacc tcttcctgtt atatagaaga attttaaacg
       61 acaagtaagg gaatataaat gaaaataaca aaaaataaaa atgcaactat aaaaaacagt
      121 catcagtcgg ctgcttttca atgtttacct tatcattttc tggtaatgct gccaacttac
      181 tgatttagtg tatgatggtg attttaaggt gcttgcgtgg cttccatttc catcagatgt
      241 ccttcctgct ccgctactga aggcgtggtg cgtaacggca aaagcactgc cggacatcag
      301 cgctatctct gctctccttg ccgtaaaaca tggcaactac agttcactta caccgcctct
      361 cagcccggta agcaccagaa aatcattgat atggccatga atggcgtcgg atgtcgcgcc
      421 agtgcacgca ttatgggcgt tggcctcaac acggttttac gtcacttaaa aaactcaggc
      481 cgcagtcggt aacctcgcgc atacaaccgg gcagtgatgt gattgtctgc gctgaaatgg
      541 acgaacagtg gggctacgtc ggtgctaaat cacgtcagcg ctggctgttt tacgcgtatg
      601 acaggatacg gaggacggtt gtggcgcacg tcttcggtga acgcactctg gccacactgg
      661 agcgtcttct gagcctgctg tcggcctttg aggtcgtggt atggatgacg gatggctggc
      721 cgctgtatga atcacgcctg aagggaaagc tgcacgttat cagcaagcgt tacactcagc
      781 gcattgagcg acataatctg aatctgagac aacatctggc aaggctggga cggaagtcac
      841 tgtcgttctc aaaatcggtg gagctgcatg acaaggtcat cgggcattat ctgaacataa
      901 aacactatca gtaagttgga gtcattacct gagaataata cctcctgggt aagtgagcag
      961 atactttcag cgcctccgaa agatgtaaat attcgacttg gagtcggttc tttagaaggt
     1021 acaaccgtct cacatgttca gcgattgcat cattcgttaa tcgcagctgg tttggaaagt
     1081 aacctcactg tctataccgg tggtcacgat tatgcctggt ggcgcggagc aattattgat
     1141 gaattagcaa attataattg caggaaggta tcaaataata actctgtgta aaatatcgtt
     1201 ttatcgtcag ataactcttt ctgcaatatt attatgggag actatctcat ttatttagtg
     1261 atgtccttca tttatgtgat gaaggacatt atattactca taacatttct cgttgaaaat
     1321 ttcagcaata cgctacaatt gtttcccgtt gttttttaat tttctcatca aaacctctgc
     1381 aatctctgca ggactgggtt gttcagccat ttcagcggct acctgatctg aacagaagcg
     1441 tagtgaatca tcataaagaa ggcgatttac caaatcactg gtcagtccat gcttgtccgg
     1501 aataatccca cacccacgct tcgcaacaat ttctgcatta acagagcgat ctgcaccttc
     1561 gccaaacaca atctgtggta ttccactata cagcgctgtc agggtattac ctgcgccacc
     1621 gtgatgaata aatccatcag ctccattaag gaatacaccc ataggtatcc agtcaaccag
     1681 acgtacattt gatggtagtt ttcgtaaacc agtacgagca tttattgcaa gttgcaaaat
     1741 gatatcagca tcaacttcat ttgcagaatc cataacccat gaaatcagct ccagaccatc
     1801 aaccattggt tttacagtgc caaggctgat aagtaaacgt ttccgatcag aatccctgtc
     1861 ccaccatgtt tcctttacag cacctccgtt ataaggaata tatctcattg agataaccgg
     1921 ttctccagca tttttaagaa tgctcatact tgggggagca acatcaatcc atgctaaatc
     1981 acatagtgga ccgctgactc catgccgcct gtaagcattg cttaaagaac ggtttaacat
     2041 ctgaatatgg gcagatgtat gcgcgaatcc aacagccagc atcactgaag gaattctata
     2101 tttagcagca accaatgggc ctgccggatc aagaggggga tagactatta aatctggcct
     2161 ccattttcct gcaaaatcga tgaggttatc tgccatttca tcgctaaaaa aagagaaatg
     2221 accaataata ttactttttt tcctcaactc ttcctgatgg cgatatccag cctctgaatc
     2281 taaacctgga actgcatcaa aaacaaccag tccagcttct gctgctttat tc
//
LOCUS       DBFIFM010000355         2311 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.357, whole genome shotgun sequence.
ACCESSION   DBFIFM010000355 DBFIFM010000000
VERSION     DBFIFM010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.357"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..1661)
                     /locus_tag="N7C74_004777"
     CDS             complement(<1..1661)
                     /locus_tag="N7C74_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGU2434471.1"
                     /translation="MNISNSQIDILRRDVRAGLRALFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNPL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLSLAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEYYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDL"
     gene            complement(1636..2181)
                     /locus_tag="N7C74_004778"
     CDS             complement(1636..2181)
                     /locus_tag="N7C74_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU2434472.1"
                     /translation="MKVNKKKLAEIFNVDPRTIERWQSQGLPCVSGGGKGVESVFDTA
                     TAIQWYAQREADIENEKLRKEVEDYRAASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSD"
BASE COUNT          526 a          655 c          562 g          568 t
ORIGIN      
        1 agatcaaaaa tatccgggtt attcgggaag tgaacggcac cgggaagcgg ttcatcccct
       61 tccggcgtca gtgtgaagcg gttataaatc tgctctttcg cggtatccgt accgatttcg
      121 gtaaggtaaa ccccgttttt gtttcgctta cgtggcatgc tggccaccgg ctttccgtag
      181 acggatgccc ctttaatggg gatcacccgg aacagcccat gttttttcga gcgttcatac
      241 acaatggtcg ggtcaatccc gccagtatcc cagcagatac gggataccga catttctgca
      301 ccattccggc gggtataggt tttattgatg gcctcatcca cacgcagcag cgtctgttca
      361 tcgtcgtggc ggcccataat aatctgccgg tcaatcagcc agctttcctc acccggcccc
      421 catccccata cgcgcatttc gtagcggtcc agctgggagt cgataccggc ggtcaggtaa
      481 gccacacgat caggaacggg cgctgaataa tactctttcc gctctgccat cacttcagca
      541 tccggacgtt cgccgatttt cgcttcccac gtctcaccga gcgtggtgtt cacgaaggtt
      601 ttacgttttc ccgtatcccc tttcgtcttc atccagtctt tgacaatctg cacccaggtg
      661 gtgaacgggc tgtacgccgt ccagatgtga aaggtcacac tgtcaggcgg ttcaatctct
      721 tcaccggatg acgaaaacca gagaatgcca tcacgggtcc agatcccggt cttttcgcag
      781 atataacggg catcagtaaa gtccagctcc tgctggcgga tgacgcaggc attatgctcg
      841 cagagataaa acacgctgga ggggtcatcc ggcgtccatt tgaggccaaa cggcgtctct
      901 ttgtcgccaa atttaagata ctgctcctcc ccgcagtgcg ggcaggcaac atgaaaacgc
      961 ataaaatgcg gggattcact ggctgcacgc tcaatctggc aggtgcctct cactttgggc
     1021 gtggagccac ggatggactt tggccagacc gagccttcaa tacgcttgtc acccaggaac
     1081 gtcggagagc cttcctgttc aatatcctca tcaaaggcag caagttcatc ataacccgcc
     1141 acatcaaccg acttttcacg gtagtttttt gccgctttac cgcccaggca ccagaagcca
     1201 cgaccattgg tgaaacgctt catagtgagc gtgttatccc ggtgcttttt gccataccac
     1261 ggggccagag acagcagcga cggaatatcg cggatggtag gctcgacgtg ggttttcata
     1321 aagttctcgg catcaccatc cgtcggcaac cagataaggg ggttacgctg cttatgctct
     1381 atgaagtagg cataaacacc cagcagcatt tttgaataac caacacgggc agacttcacc
     1441 acattcacct cgcggatgta gtcgctgccc atcgcattca tgatagcccg ctgaaagggt
     1501 agtgtttccc agcgcccttc ctggtatgcg gattctttcg ggagatagta actggcatcc
     1561 gcccattcaa cggcagtctg tggctccggc ctgaacaggg ctcgcagccc ggcgcgtaca
     1621 tcacgccgca gaatatcaat ctgactgttc gatatattca ctcagcaacc ccggtatcag
     1681 ttcatccagc gcggctgctt tgttcatggc tttgataata tcccgtttca ggaaatcaac
     1741 atgtcggttt tccagttccg gaaaacgccg ctgtactgag agagggatcc cgtcaagaat
     1801 actggcaatt tcacctgcga tccgtgacag cacgaaagta cagaatgcgg tttccaccac
     1861 ttctgcggag tctctggcat tcttcagctc ctgggcgtca gcctgcgcac gcgtaagtcg
     1921 atggcgttcg tactcaatag tcccaggctg gagatctgcc tcgctggcag ccctgtaatc
     1981 ctcaacctct ttacggagtt tttcattttc gatatcagct tccctctgcg cataccactg
     2041 aattgccgtg gcggtatcaa atacagattc aacgccctta ccacctccgg agacgcaagg
     2101 gagcccctga gactgccagc gttcaatcgt tcgcggatcc acgttgaaaa tttcggcaag
     2161 tttcttttta ttaaccttca tgaaacagtc tcacaacaaa tacagggtcc gacatgaaag
     2221 tgcccgaaaa tgactttttc aggcgttttc atgtcggacc tttacggatt cgatattaga
     2281 aaaaacaaat agttatgttc gagaagtacc g
//
LOCUS       DBFIFM010000356         2308 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.358, whole genome shotgun sequence.
ACCESSION   DBFIFM010000356 DBFIFM010000000
VERSION     DBFIFM010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.358"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..349
                     /gene="nleE"
                     /locus_tag="N7C74_004779"
     CDS             <1..349
                     /gene="nleE"
                     /locus_tag="N7C74_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase NleE"
                     /protein_id="HGU2434473.1"
                     /translation="GQFSVDSLYNPDLHALCELPDICCKIFPKENNDFLYIVVVYRND
                     SPLGEQRANRFIELYNIKRDIMQELNYELPELKAVKSEMIIAREMGEIFSYMPGEIDS
                     YMKYINNKLSKIE"
     gene            727..1704
                     /locus_tag="N7C74_004780"
                     /pseudo
     CDS             727..1704
                     /locus_tag="N7C74_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          727 a          468 c          508 g          605 t
ORIGIN      
        1 ggggcagttt tctgtagata gtttatacaa tcctgattta catgcattat gtgagcttcc
       61 ggacatttgt tgtaagatct tccctaaaga aaataatgat tttttataca tagttgttgt
      121 gtacagaaat gacagccctc taggagaaca acgggcaaat agatttatag aattatataa
      181 tataaaaaga gatatcatgc aggaattaaa ttatgagtta ccagagttaa aggcagtaaa
      241 atctgaaatg attatcgcac gtgaaatggg agaaatcttt agctacatgc ctggggaaat
      301 agacagttat atgaaataca taaataataa actttctaaa attgagtaga gaagctcagc
      361 cacttgtgta ggggggctaa aattaattct atttcattat atgagaacac caataagaat
      421 aataatattt gatcacaact gaagatgctt tttacaatga agaattattg ttaaaaaacg
      481 ctttgttgaa tcaaccgaac tgttgctgag ttgcaggatc acatcacgta ttttcctgaa
      541 aacgcaggct gcttagtagc aaagcagaag ttcaaaatca ccaactaaat agctgcgccg
      601 aatagtagat caatgaaagg gaactcagcc cggattgtgc gatctgatca atcgccaaaa
      661 atccccccct aaccggactg agcgatgccg atcatagcac caattccccg cgacgaatga
      721 cgcctgatgc aaaaagcaat ctataaaact cacgataaaa attatgcccg caggctgacc
      781 gccatgctga tgctgcaccg gagcgctcgc gtcagtgacg ttgccagaac gctctgctgc
      841 gcccgttcct ccgtcaggcg ctggattaac tggttcacgc tgtcgagtgt tgaaggtctg
      901 aaatcattac ccgcaggacg ttcccgccgc tggccgtttg agcatatctg cacattgttt
      961 cgtgaactga taaaacactc tcccggatat tttggctatc agcgttcacg ctggagtaca
     1021 gaactgctgg caataaaaat caacgagata accgagtgcc agttacatgc cggaaccgtt
     1081 cgccgctggt tgccattagt cgggcttgtg tggcgcaggg ctgcgccaac cctgcgaacc
     1141 cgtaacccgc ataaagcagc aattcacaaa gcactggacg aatgtagtgc ggagcatccg
     1201 gtcttttatg aagatgaagt gtatatctat cttaatccca aaaccggtgc ggactggcaa
     1261 cttcgcagac agcaaaaaca tgtggtaaca ccggggcaga atgaaaaata ctatctggct
     1321 ggtgctttac atagtggaac gggcaaagtc agctatgtgg gtggcaacag caaaagctca
     1381 gccttattta tcagtctgtt gaaacatctg aaattgacat accgacggga taaacctatc
     1441 acgctcatag tggacaacta catcatccat aaaagtcggg aaacacagcg ctggctgaaa
     1501 gaaaatccga agttcagagt gatttatcag ccagtttact cgccgtgggt gaatcatgtt
     1561 gagcggttat agcaggcgct tcacgatacg ataacgcgca atcatcagtg ccgttcaatg
     1621 tggtaactgc ttaaaaatgt tcattatttt atggaaaccg tgagcccatt ccccggtggt
     1681 aaatatgggc tgttaaaagt gtagcggtat tcggcgcagc tatttaggat ggtcaggtcg
     1741 cagaagttct gtgccactac agtgcgtgta ctaaacaaaa tgacgagggc acgaatgtca
     1801 taatacgtgc gtattacctg acagtagcta ccaactgagg gggcttattc caaacctgat
     1861 ttattcaaca aagctggact tatccttact ctgaattatt ttccagacat atgcatcatg
     1921 tactggctcg cttggttcag gaaatacagg agcaaatcta cagctattag atttattcct
     1981 atttgtatgc aaagtcgcat tgcataatgc ggagctatgt tacagatacg cctgacgtgc
     2041 gtagtaaaat tataaagcaa catatatata caaggagaaa ccgagaaaaa taaatgagtc
     2101 gtatgttggg tagcatcgag ataaatattt tttttatcac attagcacct tggttaaggg
     2161 tgttaataat aatattctct cacttattaa aacataaata attttttaag cggaaatgta
     2221 attatgagac tgccagagaa agttcttttt cctcctgtca ctagtggcct gtcagggcag
     2281 gaaaaacaaa aaaaaccgaa gagcatta
//
LOCUS       DBFIFM010000357         2295 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.359, whole genome shotgun sequence.
ACCESSION   DBFIFM010000357 DBFIFM010000000
VERSION     DBFIFM010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.359"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(65..709)
                     /locus_tag="N7C74_004781"
     CDS             complement(65..709)
                     /locus_tag="N7C74_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HGU2434474.1"
                     /translation="MESLGFRLKRLRKDKGLTQVELGKLSGVTGVTIGYWEKDLNEPG
                     SKALSKLAQALGTTESYLLYGVSSPELSFVQSTSGTKIPYLSWGEAISFLILKGEKTM
                     GNVDRITTFFDVEEGDFAVSMPDDTMHNPSGSPSIPVGATVILRPGESYKNGSIVAVI
                     VPDPLKNEPSMTIKKLVIDGKLVYLSPLNPRYQSSLLTPECKIVAVAKGVQFNL"
     gene            807..1034
                     /locus_tag="N7C74_004782"
     CDS             807..1034
                     /locus_tag="N7C74_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGU2434475.1"
                     /translation="MRVDELVQFFGSVQRVADFYGITREAIYMWRKRPGEIVPKGRAA
                     EAAAYSKGKLSLNPELYKKKDTTQGEGKGDS"
     gene            1031..1456
                     /locus_tag="N7C74_004783"
     CDS             1031..1456
                     /locus_tag="N7C74_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU2434476.1"
                     /translation="MKIKHEHIRMAINAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAAAIAWGTLTNSGGQPGNAVVVH"
     gene            1525..>2295
                     /locus_tag="N7C74_004784"
     CDS             1525..>2295
                     /locus_tag="N7C74_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HGU2434477.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     MKEDNGNVSQTAKSPAERKRAQRERERLRKQNTDCHDESRRVTHMSRQITTDTDTDKE
                     LNPTHNARMRESATTGELNDASLQTAEPEYLDGLSEPIGKFPMTGVWQPSPDFRQRAA
                     VWGMALPEPEFTPAELAAFRDYWMAEGKVFTQVQWEQKF"
BASE COUNT          617 a          499 c          598 g          581 t
ORIGIN      
        1 gtaaatctag attaatttaa aaaaaaggtc ggcattgccg acctcaagta aaggacgtgg
       61 cgggttataa gttgaactgt acaccttttg ctacggcaac aattttacac tctggtgtaa
      121 gtaaggatga ctgatagcgt ggattgagag ggcttaaata cacaagcttc ccatcaataa
      181 ctaatttctt tatagtcata gatggttcat ttttaagcgg atccggaact attacagcga
      241 cgatgctgcc atttttataa ctttctcctg gccttaggat cacagtagca ccaactggga
      301 tactcggtga tcctgatggg ttatgcattg tgtcatcagg cattgaaacg gcaaaatcac
      361 cttcctcgac atcaaagaat gtggtgattc tatcgacatt tcccattgtt ttctctcctt
      421 ttaagattag gaaagaaatc gcctcacccc acgaaaggta ggggatcttg gtgcctgagg
      481 tactttgtac aaaagataat tcaggagacg atactccata taggagatag gactcagtag
      541 ttcctaatgc ctgggctaac ttacttagag ctttgctacc tggttcgttt agatctttct
      601 cccagtaccc tatagtaacc ccagtcacgc ctgaaagctt acccagttct acttgggtca
      661 gccccttatc ttttctaagt ctcttaagcc tgaagccaag gctttccatc attttctcct
      721 gcaaattgaa tataaattat tttagattgc attgacctaa aaaaaattat cttgtaatct
      781 aaaaatactt agatttggga ggtaaaatgc gagttgatga acttgtccag ttttttggtt
      841 ctgttcagag ggttgccgat ttttatggga taacccgcga agctatttac atgtggcgta
      901 agcgtcctgg tgaaatagtt ccgaagggga gggctgcgga agctgctgca tactccaagg
      961 ggaaattatc tttgaaccca gaactttaca aaaagaagga taccactcag ggcgaaggaa
     1021 agggggattc atgaaaatca aacatgagca catccgcatg gcgatcaatg cctgggccta
     1081 tccggacggt gagaaagttc ctgcagctga aatagcccgg acttatttcg agctggggat
     1141 gacgttcccg gaactgtacg acgacagcca tccggaagcc ctggctcgta atacccagaa
     1201 aattttccgt tggctggata aagacacccc tgatgctgtt gaaaaaattc aggctctgtt
     1261 accggcgatc gaaaaggcaa tgccgcctct gctggtggcc cgtatgcgca gtcacagctc
     1321 tgaatattac cgtgagatcg tcgaacggag ggatcggctg gtgaaagatg tggatgattt
     1381 tgtcgcagcg gcgatcgcct ggggcaccct gactaacagt ggtggtcagc ctggtaatgc
     1441 tgttgtcgtg cattgaccaa caatattcat gccggatttc ttccggatgt tcgagggtaa
     1501 agttcggtat cagatgaggt gagtatggct aatgcctggc tcagattgtg gcatgacatg
     1561 ccaaatgatc ccaaatggcg aaccattgcc agggtctcag gacagccaat cgcaacagtg
     1621 atggcggtgt atatccacct tctggtgagt gcgtcacgaa atgtcacgac atgtcacggc
     1681 gtgtcactac gtggtcacat tgatgtcacg acggaagatt tagcaagtgc gcttgatgtg
     1741 acggaagacg tgattgattc aattttgcat gcaatgcagg ggcgagttct ggatggtgat
     1801 cttatttccg gatgggaaaa acgccaggtg atgaaggagg ataacggtaa tgtttcgcaa
     1861 accgcaaaat ccccggcaga gcgcaagaga gcgcagcggg agcgggaaag actgcggaag
     1921 cagaacactg attgtcacga tgagtcacga cgcgtcacgc atatgtcacg acaaatcacg
     1981 acagatacag atacagataa agaattaaac cccacacata acgcgcgcat gcgcgagagt
     2041 gctacgaccg gtgagttgaa tgacgcgtcg ttgcagacag ccgaacctga atacctggac
     2101 ggcctgagcg aaccgatcgg gaaatttccg atgactggag tctggcaacc gtcgccggat
     2161 tttcgacagc gggcagcagt gtggggtatg gctctgcctg agcctgagtt tacacctgct
     2221 gagcttgccg cattccggga ttactggatg gcggagggga aggttttcac gcaggttcag
     2281 tgggagcaga aattt
//
LOCUS       DBFIFM010000358         2281 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.360, whole genome shotgun sequence.
ACCESSION   DBFIFM010000358 DBFIFM010000000
VERSION     DBFIFM010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.360"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            226..432
                     /locus_tag="N7C74_004785"
     CDS             226..432
                     /locus_tag="N7C74_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU2434478.1"
                     /translation="MEKITTGVSYTTSAVGTEYWLLQLLDKVSPSQWVAIGVLGSLLF
                     GLLTYLTNLYFKIREDRRKVARGE"
     gene            complement(688..960)
                     /locus_tag="N7C74_004786"
     CDS             complement(688..960)
                     /locus_tag="N7C74_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434479.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            1120..1653
                     /locus_tag="N7C74_004787"
     CDS             1120..1653
                     /locus_tag="N7C74_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU2434480.1"
                     /translation="MNEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            1874..1987
                     /locus_tag="N7C74_004788"
     CDS             1874..1987
                     /locus_tag="N7C74_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434481.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            1989..2219
                     /locus_tag="N7C74_004789"
                     /pseudo
     CDS             1989..2219
                     /locus_tag="N7C74_004789"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
     gene            2209..>2281
                     /locus_tag="N7C74_004790"
     CDS             2209..>2281
                     /locus_tag="N7C74_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434482.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTP"
BASE COUNT          586 a          452 c          626 g          617 t
ORIGIN      
        1 tatgcctggc ggtatatttt ctgacagaca gtgacgggtg ttgtcaagat attgtgtcat
       61 ttataacctg aatcagggga ggccggaatg ttatctggca tttttagcag agcctgaatg
      121 ccataatcac ggctcccgga gttggccgtc agtgggtgac actggcggct tttttgtttt
      181 tctttacttt cattttctgt cggcggtgac ggagacatac atcagatgga aaaaatcaca
      241 acaggtgtgt catacaccac gtcagcggtg gggacggaat actggttact gcagctgctg
      301 gacaaagtct ctccgtccca gtgggtggca ataggtgtgc tgggaagtct gctgtttggc
      361 ctgctgacgt atctgactaa cctgtatttc aaaatcagag aggaccgtcg taaggtggcg
      421 cggggagagt agtcgatgaa taaacaatac gaactggttg taaaatgaat atttctaact
      481 gaaaaaacgt tccatgaggt aagaaaaggt cacaggcaat caataacagg acgtgatgaa
      541 agacccttgc atttgtgcgc tttctcttta gatagcagca gatactgaaa atctgagttg
      601 tcggggagtc agggatacag ctgtgcaaga gttggtcatt gtgattccat tgaaatcctg
      661 tatgccatga agggcaggat tttatggcta cctgagcttt ggtgatagta agttgaaaat
      721 tcgcattttt tgctgacatg cgtaacgaga atcccataag cagggaggac ttaattcttc
      781 attaacccat gcgttgatat tatgtttcag ccgttgaagc atcagcggtg ttaatgttgt
      841 ggtaataata tccagcgttt tatgtgagat cttaccgtaa gggtctgcaa gaatgctgct
      901 tgttgcttcg ttattatctg ccatcagaag aagtaactct gatttaacgt tttctgtcat
      961 tagttgtaaa aatcttctgc gcaaactttc tttactgttc atttatatgg cttcatttgt
     1021 tgtaatctgc tgcgtctcaa gggatatgtt tatgagagcg accatgagtg ttggattata
     1081 tacctaacat atcaagggat tagaaatcga taaatcccca tgaacgaaaa aataaaatac
     1141 ggcctgtcgg ctgccgttct ggcgctgatt ggtgcaggtg cttctgcgcc tgaaatcctc
     1201 gaccagtttc tggatgaaaa ggaaggtaac cacaccacgg cataccgtga tggtgcgggg
     1261 atctggacca tctgccgtgg tgccattctg gtggatggta agcctgttat tcctggcatg
     1321 aagctgtcaa aggaaaaatg cgaccgggtt aatgccatcg aacgtgacaa ggcgctggca
     1381 tgggtggaga aaaacatccg ggtgccgctg accgaacccc agaaagcggg gatcgcgtca
     1441 ttctgtccgt acaacattgg ccccggtaag tgcttcccgt cgacgtttta taaacgaatt
     1501 aatgcaggcg atcgaaaagg tgcctgtgag gcgattcgct ggtggattaa ggacggtggc
     1561 agagactgcc gtatccgttc aaataattgc tacggtcagg tctcacggcg tgaccaggag
     1621 agcgcgctgg cgtgctggga catcgacaga tagcagaata ttttcctgaa aaatgacgtt
     1681 ggccaacgcg ggtggataac acgaaatcct gaaaactggt aaaacctaag tgaataaaag
     1741 taaaaacccc gtttgttggc agcaagcggg gttttgtgtt ttctgacctt gagtaaggca
     1801 agggagaaat tatgggtagg gaggtacttt ccctgtgagg aagtataaaa gattctttct
     1861 gaggttgtcc attatgaaag gcattgaagt ggagacgcca gccagtctgg atttaacaag
     1921 agcggcagct tttgccattc gtattgtggc cattgctgtt ctggtctggg caatccgttg
     1981 gtggtgatat gaaccgtgtt ctgtgtgtgg ttatcattgt cctgctggtg gcctgtggtg
     2041 cgcttagtct ggggctgaat cattaccgtg atcacgccat catctacaaa gagcagcgcg
     2101 ataaaaaagc cagtgagctg gagctggcga acgcgacaat tactgatatg cagatacgcc
     2161 agcgtgatgt cgctgcactt gatgccagat actcgaggga attagccgat gcgagagctg
     2221 aaaatgaaac tctgcgtgct gatgttgccg ctggtcgtaa gcgcctgcgg atcaacgcca
     2281 c
//
LOCUS       DBFIFM010000359         2279 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.361, whole genome shotgun sequence.
ACCESSION   DBFIFM010000359 DBFIFM010000000
VERSION     DBFIFM010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.361"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(333..605)
                     /locus_tag="N7C74_004791"
     CDS             complement(333..605)
                     /locus_tag="N7C74_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HGU2434483.1"
                     /translation="MALKCPECGTTAHARTSAYEAPSVKRSWYQCQNLECSYTFTALE
                     SVDTIIMKPRRNEQESEKAQMPEKQQQTLNRYGSASKLSSRQQIPV"
     gene            complement(611..1162)
                     /locus_tag="N7C74_004792"
     CDS             complement(611..1162)
                     /locus_tag="N7C74_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HGU2434484.1"
                     /translation="MMARVTPDQALISFRNARILWAGHSESRKAVEQQIESLLTAMEK
                     PADYARQLELQREHLDVLKWQINCAARECIYSQHQLMEACTEDALSNFMQANGAALTS
                     ALAPFLKGRGGVDVASRILRNALVRQLAITPPEIAGDYRMILDESGVMPDPMMVRDCQ
                     SSYTPAQQLRFQQRLDYINGMQE"
     gene            complement(1159..1911)
                     /locus_tag="N7C74_004793"
     CDS             complement(1159..1911)
                     /locus_tag="N7C74_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation initiation protein"
                     /protein_id="HGU2434485.1"
                     /translation="MTIPEQNNNPIVEISIPVSTQQALEKVNATKSAWLEARRQQKAA
                     ADNIATIRQRRAEMEATTNALNEEWRTLFRESQGVVSKEMKKLRTEIALGRETLEDFD
                     ELLAAQESENALLPQEAAELAGKYIHAHDTLVGIRAKQIWEDFMQSHGKALIQTLSLL
                     KSTMGREASAVVGVVHSVNDPDTLLKDFIHKHITRPALTNDAMPEQDPVFKLAGVAPD
                     YAARLDFSNKLSPAAMHKMKVRQERAEKEKAV"
BASE COUNT          510 a          566 c          537 g          666 t
ORIGIN      
        1 tttgcaaaat tattttgggt actttcgcct ctacacttcc ttcgggtggt tccacaataa
       61 tggatctcac cggcgtttga tattttataa tctaacccct tagcgcgcgc agccccccac
      121 gcctgctcgc ttcgcttaac agactggttt tcatgcattc cgcaaatcgt ctcagaagcc
      181 accacacaag ggctttcgcg tcaaaaatgg tgcatgagaa tcatgcgttt tcatgcgcta
      241 tagacatgca ctcatgcgct ctcaggccag ccagggaaaa ggcgtaaaaa atcccagtac
      301 tggaccggga ttacgtgggc tttttttgct aatcagacag gaatttgctg acggcttgac
      361 agctttgacg cggagccata gcgattgaga gtttgctgtt gtttttccgg catttgtgct
      421 ttctctgatt cctgttcatt gcgccggggc ttcatgatta tcgtatccac actttccagt
      481 gcggtaaatg tgtaggaaca ttcaagattc tggcactgat accatgagcg tttaaccgat
      541 ggtgcttcat aggcggaggt tctggcgtgt gccgtcgtac cgcattcggg acatttaagt
      601 gccattaata ttactcctgc atgccgttaa tgtaatccag acgttgctga aagcgcagtt
      661 gttgtgcagg ggtgtaagag ctctggcaat cacgaaccat catggggtca ggcatcacac
      721 cggattcatc cagaatcata cgataatccc cggcaatttc aggcggtgtt attgccagct
      781 ggcgaaccag agcattgcgt aatatacggg acgcaacgtc aacccctccg cgccccttca
      841 ggaacggagc cagtgcagat gtcagggctg ctccattcgc ctgcataaaa ttgctgagag
      901 cgtcttctgt gcaggcctcc atcagttgat gctgcgagta aatgcattcg cgcgcagcac
      961 agttaatttg ccatttcagc acatccagat gttcccgctg taattcgagc tgtcgagcat
     1021 aatctgctgg tttttccatg gcagtgagga gtgattctat ctgttgttca acggcttttc
     1081 ggctctcact atgtccagcc cacagaatac gggcatttct aaaactgata agcgcttggt
     1141 ctggagtgac tcgggccatc atactgcttt ctccttctca gcacgttcct gacgaacctt
     1201 cattttatgc atagctgctg gagagagttt attactgaaa tccagacggg ccgcataatc
     1261 cggggcaact cccgccagtt taaacacggg gtcctgttcg ggcatcgcat cgttagtcag
     1321 agccggtctg gtgatatgtt tatgaataaa atctttcagc agtgtgtccg ggtcattaac
     1381 tgaatgcacc acaccgacaa ctgcgctggc ttccctgccc attgttgatt tgagcaggct
     1441 tagtgtctgg attagtgctt ttccatgcga ctgcataaaa tcttcccaga tttgttttgc
     1501 gcgaataccc acaagggtgt cgtgcgcgtg gatatacttt ccggctaatt ccgcagcctc
     1561 ctgcggcaaa agtgcatttt cgctttcctg agccgccagc agctcatcaa aatcctcaag
     1621 cgtttctcgt cccagcgcaa tttccgtgcg cagttttttc atttcctttg agactacgcc
     1681 ctgactttca cgaaatagcg tgcgccactc ctcattcagg gcgttcgtcg tggcttccat
     1741 ttcagcgcgg cgctggcgga ttgtcgcaat attatccgct gccgcttttt gctgacgacg
     1801 tgcttcaagc catgcagatt tggtggcatt cactttctca agcgcctgtt gcgtcgaaac
     1861 agggatagaa atttcaacga tcgggttatt gttctgttct gggattgtca tgtcagcacc
     1921 tagtggtttg tcgtggtgtc aattgtgctg tgatgcatac aaagctgcca ttgaacgcca
     1981 ttgtgcgagc gacaagacaa agactgaacc gccccggacc tcttttggct agccagaaaa
     2041 ggcctcgttt aactaaggct gtttcagact taccttaact attcactact attcacttag
     2101 gtaaaaaaaa taggtaatac agaaggttat aaggtgaagg gttggcatgg cagtattcat
     2161 cgactgttca taactgttca catgggaata aaattattta tggtattttt tcataatggg
     2221 tggttttttt attcctcgta gctatttgtt ttttgctcct caccactatt cacccttat
//
LOCUS       DBFIFM010000360         2261 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.362, whole genome shotgun sequence.
ACCESSION   DBFIFM010000360 DBFIFM010000000
VERSION     DBFIFM010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.362"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..122)
                     /locus_tag="N7C74_004794"
     CDS             complement(<1..122)
                     /locus_tag="N7C74_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGU2434486.1"
                     /translation="MTNTAKILNFGRGNFAEQERNVADLDDGYARLSNMLIEAYS"
     gene            complement(155..451)
                     /locus_tag="N7C74_004795"
     CDS             complement(155..451)
                     /locus_tag="N7C74_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HGU2434487.1"
                     /translation="MEQTNYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITNKKRPAATERSEQIQMEF"
     gene            complement(590..817)
                     /locus_tag="N7C74_004796"
     CDS             complement(590..817)
                     /locus_tag="N7C74_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2434488.1"
                     /translation="MQNLDEPIKGVGIPEVAKACGVSERAVYKWLKNGFLPKTEFFGK
                     TKYASKIEEISGGKYQASEMLEISKKNLLAA"
     gene            896..1603
                     /locus_tag="N7C74_004797"
     CDS             896..1603
                     /locus_tag="N7C74_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HGU2434489.1"
                     /translation="MVQNEKVRKEFAQRLAQACKEAGLDEHGRGMAIARALSLSSKGV
                     SKWFNAESLPRQEKMNALAKFLNVDVVWLQHGTSLNGANDEDTLSFVGKLKKGLVRVV
                     GEAILGVDGAIEMTEERDGWLKIYSDDPDAFGLRVKGDSMWPRIKSGEYVLIEPNTKV
                     FPGDEVFVRTVEGHNMIKVLGYDRDGEYQFTSINQDHRPITLPYHQVAKVEYVAGILK
                     QSRHLDDIEAREWLKSS"
     gene            1664..2005
                     /locus_tag="N7C74_004798"
     CDS             1664..2005
                     /locus_tag="N7C74_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3024 domain-containing protein"
                     /protein_id="HGU2434490.1"
                     /translation="MAFNDLEYQAVKKEVHQFIESIRPPEHIRNELDIVYSINDQTID
                     IGEQRPVWQGNPGETNILPSARIKYIRSLDRWKIYWMRKDMKWHQYSTELSLTDALEL
                     VRADPDCCFFG"
     gene            2073..>2261
                     /locus_tag="N7C74_004799"
     CDS             2073..>2261
                     /locus_tag="N7C74_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001221210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434491.1"
                     /translation="MRILGVRAAPKVTSFVVYCTNESALKCVDVIKIPSTLDTPEKLK
                     YVRNNILDILNLYNVELAA"
BASE COUNT          635 a          441 c          535 g          650 t
ORIGIN      
        1 gaataagcct caatcagcat atttgatagt ctggcgtaac catcatcgag atctgccaca
       61 ttacgctcct gttcggcaaa gttacctctg ccgaagttga gtatttttgc tgtatttgtc
      121 ataatgactc ctgtagattg atccagtaat gacctcagaa ctccatctgg atttgttcag
      181 aacgctcggt tgccgccggg cgttttttat tggtgatttc atcaagcgca tacttaaaag
      241 ctctgctaat cggactgatg tctgatgcca tgccaaaagc acacaagacc gaagctataa
      301 acctccagtc tgttctgctt atcttcgatt catgacagcc aatcatcttt gccagaccgc
      361 gctgagtaag cgttgacagg ttgataagta aatctgtttc tgcgcgatca acgtcgcgct
      421 gtgatagttt gctgtaattt gtttgttcca tttcttaaga tttccaatag tgaatagtta
      481 gttgaaaggt atgcgtggaa acgcatatgg ccttagttgg tcagatatat tgggactcgc
      541 tttgtcagcg acgtaggacg aatgtccatt gtgaaaatag cggtgttact tatgcagcca
      601 gaaggttctt tttgcttatt tcaagcattt cgcttgcttg atatttgcca ccagaaatct
      661 cttcgatttt tgatgcgtat ttagttttcc caaaaaactc agtcttaggg aggaagccgt
      721 ttttgagcca cttatagaca gccctttcgc taactccaca agccttcgca acttcaggga
      781 tgccgacacc tttaatcggc tcatcaagat tttgcatagg aatatccttt ttcgtacttt
      841 cagtacgtat tatggttgaa ctgaaagttt ttgcaagtgc tttagtatcg tactcatggt
      901 tcagaatgaa aaagtgcgca aagaattcgc ccagcggcta gcgcaagcct gtaaagaagc
      961 tggtcttgat gaacatggta ggggaatggc tatagcccgt gccctttctc tttcgtccaa
     1021 aggcgttagc aaatggttta atgctgagtc tttaccgcgt caggaaaaaa tgaatgcgct
     1081 tgcgaaattt ctaaacgttg atgttgtttg gcttcagcac ggcacttcgt taaatggagc
     1141 gaatgatgaa gatactcttt catttgttgg caaattaaaa aaagggttag tgcgcgtggt
     1201 tggtgaggca attcttggtg ttgatggtgc catcgagatg accgaagagc gcgatgggtg
     1261 gctcaaaatt tatagcgatg atccagatgc ctttggtctt cgtgtgaaag gagacagcat
     1321 gtggcccaga ataaaatcag gagaatatgt actcattgag cctaacacca aagtattccc
     1381 gggtgatgag gtgtttgtca gaaccgttga aggacacaac atgattaagg ttcttggcta
     1441 tgacagagat ggagaatacc aatttacaag cattaaccag gatcacaggc ctataacgtt
     1501 gccttatcat caagtagcaa aggtggagta tgtagctggt attctgaagc aatctcgcca
     1561 tctggatgac atcgaggcaa gggagtggct gaaaagttcg tgacttcatc gtcacatagc
     1621 tggtaaccag tggcctgaag agacgtttgg gtaaggagga tagatggcgt tcaatgacct
     1681 tgaatatcaa gcagtaaaaa aagaagttca ccaattcatt gaaagcataa ggccgcctga
     1741 acatatccgc aatgaactgg atattgttta tagcatcaat gaccaaacga tagatatcgg
     1801 cgaacagcgc cccgtgtggc agggcaatcc aggtgaaaca aacatcctgc catcagcaag
     1861 aatcaagtac atacgttctc tggatagatg gaaaatctat tggatgcgga aggatatgaa
     1921 atggcatcag tacagtactg aactttcgct gactgatgcg cttgagcttg tgcgtgctga
     1981 cccggattgc tgcttcttcg gatgagtgaa gagacgtttg gatgatggat ggtcgcagag
     2041 atgcggcctg attctaaaat aggatataaa aaatgagaat actaggtgtt agagcggcgc
     2101 ccaaagttac atcttttgtt gtatattgca ctaatgagtc tgcactcaaa tgtgttgatg
     2161 ttattaaaat accttcgacc ttagacacac cagaaaaatt aaagtatgtg agaaataaca
     2221 tcctcgacat tcttaattta tataatgttg aattagctgc c
//
LOCUS       DBFIFM010000361         2203 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.363, whole genome shotgun sequence.
ACCESSION   DBFIFM010000361 DBFIFM010000000
VERSION     DBFIFM010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.363"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..436
                     /gene="espM1"
                     /locus_tag="N7C74_004800"
     CDS             <1..436
                     /gene="espM1"
                     /locus_tag="N7C74_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM1"
                     /protein_id="HGU2434492.1"
                     /translation="GCILETVSRQSTKDIHGWVSDERTVYPSRVINQEIDNCCLQKNA
                     KISSEERKMVFSLVSKEFELTLDVKAAQSSINHIIIGNASFGKKMDALCDGMSRAVKN
                     STTDYIANVLADKFYQKHIAPGVDIVKLRNEIPGYMSRVIQG"
     gene            complement(655..1711)
                     /locus_tag="N7C74_004801"
                     /pseudo
     CDS             complement(655..1711)
                     /locus_tag="N7C74_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1943..>2203
                     /locus_tag="N7C74_004802"
     CDS             1943..>2203
                     /locus_tag="N7C74_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG"
                     /protein_id="HGU2434493.1"
                     /translation="MPFSFNLSSGNYLSTQDVEVLQRATRDHQMERLTIGERSFSVRY
                     QSAMDAFIVDPVQGELYSGLSHTELADIIRLADSVENQLNGGN"
BASE COUNT          606 a          433 c          518 g          646 t
ORIGIN      
        1 cggctgtata ctggagacgg taagcaggca gagtactaaa gatattcatg gatgggtaag
       61 tgatgaacgg acagtatatc cgtcaagggt tatcaaccaa gagattgata attgctgcct
      121 tcaaaaaaat gcaaaaattt cttctgaaga gaggaaaatg gtcttttctc ttgtaagtaa
      181 ggagtttgaa ctaactcttg atgttaaagc ggcacaaagt tctattaatc atataataat
      241 aggaaatgct tcttttggca aaaaaatgga tgctctttgc gatggtatga gccgagctgt
      301 aaaaaacagt acaacagatt acatagcaaa cgtgcttgca gacaagtttt atcagaaaca
      361 tatcgccccg ggtgttgata ttgtaaaact acgaaatgaa atcccaggtt atatgagtcg
      421 tgttatacag gggtaaggtt ggaggaggct atgcgccagt atcattggtt ttcatcctgt
      481 aaaaagatat gagtaaaata tacaacaggc gttacactat atgtatgtat ccaaataact
      541 catagttcac ctgtacagtt ataaatcgta aagaaaattg cagcgcgtcg tacgcagaaa
      601 cgtgctgtga ttgactattt atagctttgt cacaacgaca agattcagta gtgtttacac
      661 gggcgggaga aatgacggaa taactaaata gctgcgccta atatcgcaca tttttgccag
      721 cccatgtttg ccttcgggga atggactgac ggattccata aaatggcgaa ctttttcaat
      781 agttgccaca ttgagcggca ctgatgatta cgcgttattg tggcgtgaag tatctgccat
      841 agctattcaa aatgattcac ccatggagat taaaccggct gataaatgac tctgcacttc
      901 ggtttatcct tcagccagcg ctgggtttcc cggcctttgt ggataatgta gttgtcctcg
      961 agcagcgtga tggttttcgt ccgacagtat gtagctttaa gccgcttcag cagtctgatg
     1021 aacagcgccg aacttttgct gttggcgccc acacagctga cttaacctgt cccgctgtgc
     1081 agcgctacgt ccagagaata tttttcattt tgttccggcg tgacccctcg tttttgcttc
     1141 ccgagcagtt tccagtccgc accgattttg ggattaagat ggataaatac ttcatcttca
     1201 taaaagaccg gatgctctgt gctgcattcg tccagtgcgt tatggattgt tgccatcttt
     1261 ttatctttag gcggggcacg gatacgtaga gttggcgcag cccttcgcca cacaagcccc
     1321 acagacgaca accagcggcg aatggttcca gcatgtaaca ggcagccggt tatctcattg
     1381 atttttattg tcagcagttc tgtactccag cgtgaataat tgtagccaaa gtcgcgagtg
     1441 aatattttac ctgttcacgt aatagtgtgt agatatgcgc aaacggccag cggcgggctc
     1501 gctcggcagg taatgatttc agtccctcaa cacccgactg tgtgaactag ttaatccagc
     1561 gcccaacaga ggaacgggcg cagcatagtg tgcgggcaac gtcgctgaca cggtcgcccc
     1621 ggtgcagcat cagcatggct gtcagccttt tatggatggc tttctgtatt aggcgtcgtt
     1681 cgtcacggga aattggtggt atgatcgaca ttgctcagtc cggttggtga tttgtattga
     1741 tttggcgatt gatcagatcg cacaatcggg ttgagttccc tctaaatgat ctactattct
     1801 gcgcagctat ttagtgagta tatattttat gcttataaat tagattaaaa taatcctaca
     1861 attattctga tggagtattg ctgtggagga tggctttttg ttaattatcg aaaagtaaga
     1921 tatatgcttt agggataaaa ttatgccatt ttcatttaat ttgtcttctg gaaattactt
     1981 gtcaacacaa gatgttgaag ttttacaacg tgcgaccaga gatcatcaaa tggaaagact
     2041 cacaatagga gaaagatcgt tttctgttcg ctatcagagt gctatggacg cttttattgt
     2101 agatcctgtt caaggggaac tatattcggg tttaagccat acagaactag ccgatatcat
     2161 tagattggct gattctgttg aaaatcaatt gaatggaggc aat
//
LOCUS       DBFIFM010000362         2190 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.364, whole genome shotgun sequence.
ACCESSION   DBFIFM010000362 DBFIFM010000000
VERSION     DBFIFM010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.364"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            43..444
                     /gene="eptO"
                     /locus_tag="N7C74_004803"
                     /gene_synonym="gspS"
     CDS             43..444
                     /gene="eptO"
                     /locus_tag="N7C74_004803"
                     /gene_synonym="gspS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspS family T2SS pilot lipoprotein variant EptO"
                     /protein_id="HGU2434494.1"
                     /translation="MMGNILKKLNCIASLLVLVTISGCHQSPSIHKQATVPPSEQLEQ
                     MASIVSATRYLKMRCNRSDLPDEQSILNVANRIAIGKGWQSLTQEDIRKHSDDIYVRL
                     TRDSTPEYIKCREFNRRLVPFIGELLARGRG"
     gene            623..1794
                     /locus_tag="N7C74_004804"
     CDS             join(623..872,872..1794)
                     /locus_tag="N7C74_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611522.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU2434495.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQNYTVADAAKAMDIGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEIFKKGYNRALDVRLPEQFSI
                     IGKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHNISHGSAGA
                     RSIATMATLRGFRMGRWLAGRLMKELGLVSCQQPAHRYKRGGREHVTIPNHLGRQFAV
                     TEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTIKALKMAWEI
                     RSKPAGVMFHSDQGSHYTSRQFRQLLWRYQIKQSLSRRGNCWDNSPMERFFRSLKNEW
                     IPVTGYMNFSDAAHEITDYIVGYYNALRPHEYNGGLPPNESENRYWKNSKAVASFC"
BASE COUNT          641 a          470 c          553 g          526 t
ORIGIN      
        1 ctgttgtaaa ccggtatcag gttaaacgat acctagtaaa aaatgatggg taatattttg
       61 aaaaaactga attgtatcgc gtcattacta gtcttagtga ctatctctgg ttgtcatcag
      121 tcaccctcca tccataagca ggccactgtt cctccgagcg aacaactgga acaaatggcg
      181 tcgattgtct ctgcgacacg gtatctgaaa atgagatgta accgtagtga tttaccagat
      241 gaacagtcga tcctaaatgt cgctaaccgg atcgctatcg ggaaagggtg gcagtcatta
      301 actcaggagg atatacgtaa acacagcgat gatatctatg tgcgtctgac tagggacagt
      361 acgcctgagt atataaaatg cagagaattc aatcggcggc tggtcccatt tatcggtgaa
      421 ttattggcaa ggggtcgtgg ataatatgaa ccgccccgga aatcctggag actaaactcc
      481 ctgagaaaga ggtaaacagc atgaataaaa atgggtcttc ccttgtagtg gtggctgaag
      541 gcatgatgat tgtagtggca cactgaattt ggccacctga acagaggtga tatgctcacc
      601 tcagaacaac acaggtgctc caatgaaaaa aagaaatttc agcgcagagt ttaaacgcga
      661 atccgctcaa ctggttgttg accagaacta cacggtggca gatgccgcca aagctatgga
      721 tatcggcctt tccacaatga caagatgggt caaacaactg cgtgatgagc gtcagggcaa
      781 aacaccaaaa gcctctccga taacaccaga acaaatcgaa atacgtgagc tgaggaaaaa
      841 gctacaacgc attgaaatgg agaatgaaat attaaaaaag gctacaaccg cgctcttgat
      901 gtcagactcc ctgaacagtt ctcgataatc gggaaactca gagcgcatta tcctgtggtc
      961 acactctgcc atgtgttcgg ggttcatcgc agcagctaca gatactggaa aaaccgtcct
     1021 gaaaaaccag acggcagacg ggctgtatta cgcagtcagg tacttgagtt gcataacatc
     1081 agccatggtt ctgccggggc aagaagcatc gccacaatgg caaccctgag aggcttcaga
     1141 atggggcgct ggcttgccgg caggctcatg aaagaactgg gactggtcag ttgccagcag
     1201 cctgcgcacc gttataaacg aggtggtcgt gaacatgtca ctatcccgaa tcaccttggg
     1261 cggcagttcg cagtgacaga gccaaatcag gtatggtgcg gcgacgtgac gtacatctgg
     1321 acggggaaac gttgggcata ccttgccgtt gttctcgacc tgtttgcaag gaaaccggta
     1381 ggttgggcaa tgtcgttctc tccggacagc agactgacca tcaaagcgct gaaaatggcc
     1441 tgggaaatcc gcagtaaacc agccggggta atgttccaca gcgatcaggg cagccactat
     1501 acaagcaggc agttccggca gttactgtgg cgttaccaga tcaaacagag tctgagtcga
     1561 cgaggaaatt gctgggataa cagcccgatg gagcgcttct tcaggagtct gaaaaacgag
     1621 tggataccgg tgacgggtta catgaacttc agcgatgctg cccatgaaat aacggactat
     1681 atcgttgggt attacaacgc gctcaggccg cacgaatata acggtgggtt gccaccaaat
     1741 gaatcggaaa accgatactg gaaaaactct aaagcggtgg ccagtttttg ttgaccacta
     1801 catcccgtca gccgatttac gggccgtaag taacgaagtt ggatgcaaat gtcagatcgt
     1861 gtgcgcctgt tatggctcgg ctggtaagag agccttatag gcgcatttga aaaaactcca
     1921 gctataccgg aagagattta ttattaacag gagaggtttg cttttctgtg taggtttaga
     1981 ttttgtgttt gcagggtaaa caaaactgat taactcgtca tttcggtaac tgaaatagtt
     2041 tgctgtattc tgtttacaat ctctttaata aggcatttgt cacgtggcta ttcatatgaa
     2101 aatcatacgt tatcttacaa atcaatcatc tgagtgttat aatataactt agctgtgata
     2161 tgtgtaagaa tgtttaggca atatattatt
//
LOCUS       DBFIFM010000363         2130 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.365, whole genome shotgun sequence.
ACCESSION   DBFIFM010000363 DBFIFM010000000
VERSION     DBFIFM010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.365"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..177
                     /locus_tag="N7C74_004805"
     CDS             <1..177
                     /locus_tag="N7C74_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2434496.1"
                     /translation="SILDDVRLNNALSAIQTTVKPISEIARENGYKCPSRFTERFHNR
                     FNITPREIRKASRE"
     gene            294..395
                     /locus_tag="N7C74_004806"
     CDS             294..395
                     /locus_tag="N7C74_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434497.1"
                     /translation="MRTYNPNSLLPSQMQKCTCNSLHLAFDLCGGEA"
     gene            392..847
                     /gene="ybcN"
                     /locus_tag="N7C74_004807"
     CDS             392..847
                     /gene="ybcN"
                     /locus_tag="N7C74_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HGU2434498.1"
                     /translation="MNLSQDGIKLHRGNFTAIGRQIQPYLEEGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKTFATPAWVKDALKHTYLGYETKDLVDVVTGDITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQQLRDKQEA"
     gene            847..1017
                     /locus_tag="N7C74_004808"
     CDS             847..1017
                     /locus_tag="N7C74_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGU2434499.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            1010..1300
                     /locus_tag="N7C74_004809"
     CDS             1010..1300
                     /locus_tag="N7C74_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HGU2434500.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNSETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            1297..1659
                     /gene="rusA"
                     /locus_tag="N7C74_004810"
     CDS             1297..1659
                     /gene="rusA"
                     /locus_tag="N7C74_004810"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HGU2434501.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            1656..1796
                     /locus_tag="N7C74_004811"
     CDS             1656..1796
                     /locus_tag="N7C74_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HGU2434502.1"
                     /translation="MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            1882..>2130
                     /locus_tag="N7C74_004812"
     CDS             1882..>2130
                     /locus_tag="N7C74_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU2434503.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPEKVKSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMA"
BASE COUNT          566 a          483 c          549 g          532 t
ORIGIN      
        1 agtatactgg acgatgtgag gttaaataat gcgttgtctg ctatacaaac gacggtaaaa
       61 cctataagcg agattgccag agaaaatggt tataagtgtc cttcgcgttt tactgaaaga
      121 tttcataatc gttttaatat aacaccaaga gagataagaa aagcttccag agagtaaaag
      181 tgttttaaga aggagcaatt ctatcgattt tgattttggg aaatcaacac ggcataatta
      241 tgtcaccgga gcctgaacaa ctccggtgac ttctgcgcta aacggggacg tttatgcgca
      301 catacaatcc aaactctctt ctcccttcac agatgcagaa atgcacctgc aattctttgc
      361 atctagcgtt tgacctctgc ggaggtgaag cgtgaacctc tcacaagacg gcatcaaatt
      421 acatcgcggc aacttcaccg ctatcggtcg gcagatccag ccttatctgg aggagggcaa
      481 atgctttcgc atggtgctta aaccgtggcg tgagaaacgc agtctttccc agaatgcact
      541 cagccacatg tggtacagcg aaatcagtga atacctcatc agcaggggta aaacgttcgc
      601 cactccagct tgggtaaaag atgctctcaa acacacatat ctcggttatg aaaccaaaga
      661 cctggttgat gtcgtaaccg gtgatatcac cactatccag tcgttacgcc atacctccga
      721 tcttgatacc ggagagatgt atgtcttcct gtgtaaggtt gaagcctggg cgatgaatat
      781 tggttgccat ctgactattc cgcagagctg cgagttccag cagctccgtg acaagcagga
      841 ggcgtaatgg ctacaccgct tattcgtgtc atgaacggac acatctacag agtatcaaat
      901 cgtcgtaagc gtaagcctga gctgaagcca tccgaaatac caacactgct cggatatacc
      961 gctagcctgg ttgataaaaa atggttgcga ctggcagcaa ggaggaatca tggctgattt
     1021 gagaaaagca gcgcgtggtc gggaatgcca ggtaagaatc cctggcgtat gtaatggcaa
     1081 ttctgaaacg tctgtactgg cacatatccg gctggctgga ttgtgcggta ccggtatcaa
     1141 accgccagac ctgattgcca ccattgcatg ttctgcctgc cacgacgaaa tcgaccgccg
     1201 cacacatttt gtcgatgctg catatgcaaa agaatgcgcg ctggaaggta tggcgagaac
     1261 acaggttatc tggctgaaag agggggttat taaggcgtga atacctacag tatcacatta
     1321 ccctggcctc cgagcaataa tcgctattac cgccataatc gcgggcgcac gcacgtcagt
     1381 gcagaggggc aggcataccg cgataacgtc gcccgaatca ttaaaaacgc aatgctggat
     1441 atcggcctgg ctatgcctgt gaaaatccgc attgagtgcc acatgccgga tcgccgtcgc
     1501 cgtgacctgg ataatctgca aaaagccgct tttgacgcac tcactaaagc aggtttctgg
     1561 ctggatgatg ctcaggtcgt tgattaccgc gttgtgaaga tgcctgttac caaaggtggg
     1621 aggctggaac tgaccatcac cgaaatgggg aatgaatgat gtttgagttt aatatggcag
     1681 aacttcttcg ccaccgctgg gggcgtctgc gcttatatcg tttccccggt tctgttttga
     1741 ccgattaccg aatactgaag aattacgcca aaaccctgac aggagcagga gtatgaagtc
     1801 agagataaca atcaactaat actgttttgt tgatttttgc ttgtaattgg cgttctggtc
     1861 tgatttttgt ggagtaagtt gatgcgtgat attcagatgg ttcttgagcg ttggggagcg
     1921 tgggcggcta ataatcatga agatgtgacc tggtcgtcca ttgccgccgg ttttaaggga
     1981 ctgatccccg aaaaagtaaa atcacgcccg cagtgctgtg acgatgatgc gatggtgata
     2041 tgcgggtgta tagcccgcct ttaccggaac aatcgcgatc tgcatgactt gctggttgat
     2101 tattacgtgt tgggggagac gttcatggcg
//
LOCUS       DBFIFM010000364         2079 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.366, whole genome shotgun sequence.
ACCESSION   DBFIFM010000364 DBFIFM010000000
VERSION     DBFIFM010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.366"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            102..257
                     /gene="ydfA"
                     /locus_tag="N7C74_004813"
     CDS             102..257
                     /gene="ydfA"
                     /locus_tag="N7C74_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU2434504.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            259..387
                     /gene="ydfB"
                     /locus_tag="N7C74_004814"
     CDS             259..387
                     /gene="ydfB"
                     /locus_tag="N7C74_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HGU2434505.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSND"
     gene            399..788
                     /locus_tag="N7C74_004815"
     CDS             399..788
                     /locus_tag="N7C74_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434506.1"
                     /translation="MEFKDLLKEIQEIAAHALHQRLNEVELESATKKYIDNMARNVRD
                     AFTGLYSVSVTNNQNTEETAKRIASVMGFHVEEKYSKKEFWKTTKKLQSENCHLLRQS
                     LLSMRKVIQMTQDYRNCSHYLKNSELS"
     gene            complement(975..1160)
                     /locus_tag="N7C74_004816"
     CDS             complement(975..1160)
                     /locus_tag="N7C74_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434507.1"
                     /translation="MPNKPCPACNALSGFLEKGGYYIFNCPEHVEFHISKLDSIITKP
                     NQYQSDLLNKELNAARD"
     gene            1734..1922
                     /gene="dicB"
                     /locus_tag="N7C74_004817"
     CDS             1734..1922
                     /gene="dicB"
                     /locus_tag="N7C74_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU2434508.1"
                     /translation="MEKLSCNASTSELRFEIGVITGDKTFIEDAIKQRKLEQDLLNEV
                     CIPSMLARLDLLQKGYKQ"
     gene            1919..>2079
                     /locus_tag="N7C74_004818"
     CDS             1919..>2079
                     /locus_tag="N7C74_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGU2434509.1"
                     /translation="MNTTFALVLTVYLVSGESLELVTGLYGSMKECMAAAAEQKIPGN
                     CYPVDKTTHT"
BASE COUNT          618 a          438 c          482 g          541 t
ORIGIN      
        1 gccagcccga ggcgtatgaa catgacggca gggttcaact ttaataacta tgcagcaggt
       61 ttttgttccg ctacccggcg ttaaggggaa atgaggtcaa catggatact atcgatcttg
      121 gcaacaacga atctctggtg tacggcgtgt ttcccaacca ggacggtaca ttcaccgcga
      181 tgacgtatac caaaagcaaa acgtttaaaa ccgaaaatgg tgcccgtcgc tggctggaaa
      241 gaaactcagg tgagtgatat ggatttcgac acaatcatgg aaaaggctta cgaagaatac
      301 ttcgaaggcc ttgccgaagg cgaggaagct ctcagcttca gtgagtttaa acaggcgctt
      361 tccagttcgg caaaatctaa cgactaacgg agttaaagat ggaatttaaa gatttactaa
      421 aagaaatcca ggaaattgca gcacatgcac ttcaccaacg tctgaacgaa gttgaattag
      481 aatctgcaac gaagaaatac attgataata tggctcgtaa tgtgcgcgat gcgtttaccg
      541 gattgtactc tgtttcggta acaaacaacc agaatactga agaaactgca aagcggattg
      601 cctcggtgat gggttttcat gtcgaggaaa agtattcaaa aaaagaattc tggaaaacta
      661 caaaaaaatt gcagagcgag aactgtcatc tcctgcggca atcgcttctt tctatgagga
      721 aagttattca gatgacacag gattaccgga actgctctca ttacttgaaa aattcggaat
      781 tatcatgaaa gggactgcgt cggcggattt cacatactca atgatggctg ctgatggaga
      841 gccttttcct cccgaactta aaataacccc cggcaatcat ccgatggtaa caataagagc
      901 aatagagaat tactggttta atccagttca acaatagttg gtctttgcaa tatttttaat
      961 gggccactac gcccttaatc tcgggcggcg tttaactctt tattcagtaa atcagactga
     1021 tactgatttg gctttgtaat gatactgtca agttttgata tatgaaattc aacatgttct
     1081 ggacagttaa aaatgtaata accgcccttc tctaaaaagc cagaaagagc gttacaggca
     1141 gggcatggtt tgtttggcat tttatcctcc attaagggct gaattaaaaa tggagaccaa
     1201 cacgctgcta cgtgtggtcg tgcgccggac acagataaga atccggcact gacagtttac
     1261 tgaaaggata tatccctgaa aagtcagggc ataacacgaa agtgcacggc gaagtccttc
     1321 tcccttagag ccgtcgttaa atttaattcg accgtgcgct tccggttgtg gcaatccgcg
     1381 aaatggcgcg gcggtaagta tggcggggtt attctttccc cgttgaggac accgggttgt
     1441 caggttgacc atacgcttaa gtgacaaccc cgctgcaacg ccctctgtta tcaatattct
     1501 ggtgacattt ggcggtatca gttttactcc gtaactgctc tgccgccctt ttttaaaagt
     1561 gaattttgtg atgcggtgaa tgcggctcag cgcacgcgga acagttaaat cggtaaagcg
     1621 gtcatttgct gagtaacgag aatgctctgt atccggcgtt aattgttaac tggttaacgt
     1681 cacctggagg caccaggcac cgcatcacaa aattcattgt tgaggacgcg ataatggaaa
     1741 agttatcatg taatgccagc acgtctgaac ttcgtttcga aattggcgtt atcactggag
     1801 acaaaacatt tattgaagac gccattaagc agagaaaact cgagcaggac ctgttaaatg
     1861 aagtatgcat tccttcaatg ctggctcgtc tggacctgct gcaaaaagga tataaacaat
     1921 gaatacaaca tttgcactcg ttctgacagt ttatcttgtt tccggcgaat ctcttgagct
     1981 ggtgactggc ttatacggtt caatgaaaga atgcatggct gcagcagcag aacaaaaaat
     2041 tcccggtaac tgttatccgg tagataaaac tactcacac
//
LOCUS       DBFIFM010000365         2061 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.367, whole genome shotgun sequence.
ACCESSION   DBFIFM010000365 DBFIFM010000000
VERSION     DBFIFM010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.367"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..353)
                     /locus_tag="N7C74_004819"
     CDS             complement(<1..353)
                     /locus_tag="N7C74_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434510.1"
                     /translation="MKKAAILIDAGFFMQRVHATHRKHFAEHELTAQCIMKVIWSMVL
                     SHLNGKRQSQERREPLELYRIYFYDCPPLDIQTRLPLPEPGNKTPGRKNFKLEKSYIL
                     RTELHEELRKTRKTA"
     gene            complement(481..1194)
                     /locus_tag="N7C74_004820"
     CDS             complement(481..1194)
                     /locus_tag="N7C74_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HGU2434511.1"
                     /translation="MSAKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMG
                     QSGVGALFNGINALNAYNAALLAKILNVSVEEFSPSIAREIYEMYEAVSMQPSLRSEY
                     EYPVFSHVQAGMFSPELRTFTKGDAEKWVSTTKKASGSAFWLEVEGNSMTAPTGSKPS
                     FPDGMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNE
                     SCSVVGKVIASQWPEETFG"
     gene            1295..1495
                     /locus_tag="N7C74_004821"
     CDS             1295..1495
                     /locus_tag="N7C74_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGU2434512.1"
                     /translation="MEQRITLKDYAMRFGQTKTAKDLGVYQSAINKAIHAGRKIFLTI
                     NADGSVYAEEVKPFPSNKKTTA"
     gene            1614..1907
                     /locus_tag="N7C74_004822"
     CDS             1614..1907
                     /locus_tag="N7C74_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HGU2434513.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVAAILTNKKRPAATERSEQIQMEF"
     gene            1940..>2061
                     /locus_tag="N7C74_004823"
     CDS             1940..>2061
                     /locus_tag="N7C74_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGU2434514.1"
                     /translation="MTNTAKILNFGRGNFAGQERNVADLDDGYARLSNMLLEAYS"
BASE COUNT          581 a          463 c          456 g          561 t
ORIGIN      
        1 atggctgttt ttcgagtttt tcttaactct tcatgcagct ccgttctcag aatatatgat
       61 ttttcgagtt tgaaattctt gcgaccaggc gtcttattgc caggctcagg aagtggaagg
      121 cgtgtttgaa tgtcgagtgg tggacagtca tagaagtaaa ttctataaag ctcaagcggt
      181 tccctacgtt cttgtgattg acgttttcca ttaagatggg aaagaaccat tgaccatatt
      241 actttcatta tgcattgcgc agtcagttca tgctcggcga agtgtttacg atgcgtagca
      301 tgaacacgct gcatgaaaaa acccgcatca attaaaattg ctgctttctt cataagagtc
      361 ccaaaaaaat agcccagagc cgttatgcag atattaacaa ttgtctgcga acggggctgg
      421 gcttggttta attaatctat gccaagttga ctgtgccgtc aacataaaac ttacttccta
      481 tcacccaaac gtctcttcag gccactgact agcgataact ttccccacaa cggaacaact
      541 ctcattgcat gggatcattg ggtattgtgg gtttagtggc tgtagaaaca cctgaccgct
      601 atccctgatc agtttcttga aggtaaactc atcaccacca agtctggcta tacagaaatc
      661 gcctggctca acagcctgct cagggtcaac cagaattaac atcccgtcag gaaagctggg
      721 cttggatcct gttggtgcgg tcatggaatt accttcaacc tcaagccaga atgcagagcc
      781 actggctttt ttggttgtgc ttacccattt ctccgcatcg cctttggtaa aggttctaag
      841 ctcaggcgag aacatcccgg cctgaacatg agaaaaaaca gggtactcat actcacttct
      901 aagtgacggc tgcatactaa ccgcttcata catctcgtag atttctctgg cgattgaagg
      961 gctaaattct tcaacgctaa cgttgagaat ttttgcaagc aatgcggcgt tataagcatt
     1021 taatgcattg atgccattaa ataaagcacc aacgcctgac tgccccatcc ccatcttgtc
     1081 tgcgacagat tcctgggata agccaagttc atttttcttt ttttcataaa tagctttaag
     1141 gcgacgtgcg tcctcaagct gctcttgtgt taatggtttc ttttttgcgc tcatacgtta
     1201 aatctatcac cgcaagggat aaatatctaa caccgcgcgt gttgactgct ttacctctgg
     1261 cggtgataat ggttgcatgt actaaggagg ttgtatggaa caacgcataa ccctgaaaga
     1321 ttatgcaatg cgctttgggc aaaccaagac agctaaagat ctcggcgtat atcaaagcgc
     1381 gatcaacaag gccattcatg caggccgaaa gattttttta actataaacg ctgatggaag
     1441 cgtttatgcg gaagaggtaa agcccttccc gagtaacaaa aaaacaacag cataaataac
     1501 cccgctctta cacattccag ccctgaaaaa gggcatccaa ttaaaccaca cctatggtgt
     1561 atgcatttat ttgcatacat tcaatcaatt gttatctaag gaaatactta catatggttc
     1621 gtgcaaacaa acgcaacgag gctctacgaa tcgagagtgc gttgcttaac aaaatcgcaa
     1681 tgcttggaac tgagaagaca gcggaagctg tgggagttga taagtcgcag atcagcaggt
     1741 ggaagagaga ctggattcca aagttctcaa tgctgcttgc tgttcttgaa tggggggtcg
     1801 ttgacgacga catggctcga ttggcgcgac aagttgctgc gattctcacc aataaaaaac
     1861 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     1921 tctatcaaca ggagtcatta tgacaaatac agcaaaaata ctcaacttcg gcagaggtaa
     1981 ctttgccgga caggagcgta atgtggcaga tctcgatgat ggttacgcca gactatcaaa
     2041 tatgctgctt gaggcttatt c
//
LOCUS       DBFIFM010000366         2025 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.368, whole genome shotgun sequence.
ACCESSION   DBFIFM010000366 DBFIFM010000000
VERSION     DBFIFM010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.368"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..1536
                     /gene="zorA2"
                     /locus_tag="N7C74_004824"
     CDS             <1..1536
                     /gene="zorA2"
                     /locus_tag="N7C74_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorA2"
                     /protein_id="HGU2434515.1"
                     /translation="NIHSKFREYAPTLMGALGILGTFIGIIIGLLNFNTESIDTSIPV
                     LLGGLKTAFITSIVGMFFAILFNGMDAFFFANKRSALAENNPESVTPEHIYHELKEQN
                     QTLTKLVSGINGDSEGSLIAQIKLLRTEISDSSQAQLANHTHFSNKLWEQLEQFADLM
                     AKGATEQIIDALRQVIIDFNQNLTEQFGENFKALDASVKKLVEWQGNYKTQIEQMSEQ
                     YQQSVESLVETKTAVAGIWEECKEIPLAMSELREVLQVNQHQISELSRHLETFVAIRD
                     KATTVLPEIQNKMAEVGELLKSGAANVSASLEQTSQQILLNADSMRVALDEGTEGFRQ
                     SVTQTQQAFASMAHDVSNSSETLTSTLGETITEMKQSGEEFLKSLESHSKELHRNMEQ
                     NTTNVIDMFSKTGEKINHQLSSNADNMFDSIQTSFDKASAGLTSQVRESIEKFALSIN
                     EQLHAFEQATEREMNREMQSLGNALLSISKGFVGNYEKLIKDYQIVMGQLQALISANK
                     HRG"
     gene            1543..>2025
                     /locus_tag="N7C74_004825"
     CDS             1543..>2025
                     /locus_tag="N7C74_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HGU2434516.1"
                     /translation="MDKIIGKQLPKKDQDNEHWVSMSDLMAGLMMVFMFISIAYMHYV
                     RIEKEKIKEVAVAYENAQLQLYNALDIEFAKDLQDWDAEIDKQTLEVRFKSPDVLFGL
                     GSTELKPKFKLILDDFFPRYLKVLDNYQEHITEVRIEGHTSTDWTGTTNPDIAYFNNM
                     A"
BASE COUNT          688 a          364 c          402 g          571 t
ORIGIN      
        1 aatatacata gtaagtttag agaatatgct ccaaccttaa tgggggcatt aggtattctg
       61 ggtactttca ttggtattat tattggttta ctcaatttta ataccgaaag tattgatacc
      121 agcatccccg tattattagg tggcctaaaa acagcattca ttacaagcat tgtaggtatg
      181 ttttttgcca ttttatttaa tggaatggat gctttctttt ttgccaataa acgaagtgcg
      241 ttagcagaaa ataaccctga atctgttaca cctgaacata tctatcatga attaaaagag
      301 cagaaccaga ctctgactaa attagtctcg ggtattaacg gtgatagtga aggttctctt
      361 attgctcaaa taaaattact acgtactgag attagcgatt cctcgcaggc acaattagct
      421 aatcacactc atttcagtaa taagctttgg gaacaacttg aacaatttgc agatctaatg
      481 gcaaaaggtg ctacagaaca aattattgat gctttgcgac aagtcattat tgattttaat
      541 caaaatttaa ctgaacagtt tggtgaaaac tttaaagctc ttgatgcctc tgtaaaaaaa
      601 cttgttgagt ggcagggaaa ttataaaacg caaattgagc agatgtcaga acaatatcaa
      661 caaagtgtcg agtccctggt tgaaacaaaa actgcggttg cagggatttg ggaagaatgt
      721 aaagaaattc ctctggctat gtctgaactg cgtgaagtgc ttcaggtgaa ccaacatcaa
      781 atcagcgaac tctcccgcca tttagaaacc tttgtcgcca tccgcgataa agctacaacc
      841 gtattacctg aaatacagaa caaaatggct gaagtgggtg aactgctgaa atccggagct
      901 gcaaatgtta gtgcatctct tgagcaaacc agccagcaaa tacttcttaa tgcagattca
      961 atgcgcgttg ccctggatga aggtaccgaa ggattcagac aatcggttac ccaaacacaa
     1021 caagcatttg cctcgatggc acatgatgtc agcaattcct ccgaaaccct aaccagcacg
     1081 ttaggtgaaa caattactga aatgaaacaa agtggtgaag aattcctgaa atcactagag
     1141 tcgcactcga aagaattgca tagaaatatg gaacaaaata cgacgaatgt gattgatatg
     1201 ttcagtaaga ctggtgaaaa gattaaccat caactatcca gtaatgccga taatatgttt
     1261 gattcaatcc agacatcatt tgataaggca agtgcagggc tgacttctca agtcagagaa
     1321 tcaattgaaa aatttgctct atccatcaac gagcagttac atgcttttga gcaagcaact
     1381 gaacgtgaaa tgaaccgtga aatgcaatca ttaggtaatg ctctgctttc aatcagcaaa
     1441 ggttttgtcg gtaactatga aaaacttatt aaagattacc aaatagttat ggggcagtta
     1501 caagcattaa tttctgctaa taaacatcgc gggtaatcga tcatggataa gattataggg
     1561 aaacaattac ctaaaaaaga tcaagataat gaacattggg tatccatgtc ggacctaatg
     1621 gctgggctga tgatggtttt tatgttcata tctattgctt atatgcacta cgtacgtatt
     1681 gaaaaagaaa aaattaaaga agttgccgta gcctacgaga atgctcagtt acagctttat
     1741 aatgctctgg atattgagtt tgcaaaggat ttacaagact gggatgcaga gatcgataaa
     1801 cagactctgg aggttcgatt taaatcaccg gatgttttat ttggcttagg aagcacagag
     1861 ctaaaaccaa agtttaaact cattcttgac gacttctttc ctcgctacct aaaagttcta
     1921 gataattatc aggaacatat tactgaagtc cgcattgaag gtcacacaag tactgactgg
     1981 acaggaacaa cgaatcctga tattgcttat tttaataata tggca
//
LOCUS       DBFIFM010000367         2009 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.369, whole genome shotgun sequence.
ACCESSION   DBFIFM010000367 DBFIFM010000000
VERSION     DBFIFM010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.369"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(2..691)
                     /locus_tag="N7C74_004826"
     CDS             complement(2..691)
                     /locus_tag="N7C74_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HGU2434517.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQPTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            796..1026
                     /locus_tag="N7C74_004827"
     CDS             796..1026
                     /locus_tag="N7C74_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU2434518.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAHQIRPDLPKLFPHTEKNAA"
     gene            1096..1635
                     /locus_tag="N7C74_004828"
     CDS             1096..1635
                     /locus_tag="N7C74_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HGU2434519.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAEDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            1632..>2009
                     /locus_tag="N7C74_004829"
     CDS             1632..>2009
                     /locus_tag="N7C74_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGU2434520.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNALIAGGIMANTAEIFNFPVPD
                     VAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKR"
BASE COUNT          549 a          483 c          463 g          514 t
ORIGIN      
        1 actatccatg cttcctatac gtctgcggca tgctcccaat aactttcccg aagataaaca
       61 cccggttcat ctcgtctttc tcgattgggt cccacggtga gtagctcttg ttatcagaga
      121 taaccagcag tttatccttc atcatttgca ggcgctttac atgggctgtg tcgtcgtaca
      181 gaaacgcata gataccatca ccgtcgaaag atttaaccgt gatatcaacg aacagcagat
      241 caccaggttc aatcgttcct gacatgctgt caccgcgtac gttaatgatg cggatatttt
      301 ctgccttcct gccatcgaac atgtgacgag catcgtcaaa cgagtactca accgagcgta
      361 ggacttctac aaactcacgg ttgatgacac ccggcccggc actcacttct atatcaagaa
      421 cgtcaatctt aaagtatttg gaatggctga cagttggttg tattggttgc actgtactgt
      481 ctgacatatt tccaacgcca gaagataacc attctgcgcg cacacccaaa gcgttcgcga
      541 tctccacgat tttagttgtt tgattagctt tccctgtttc gattttctga atagcagctt
      601 ggctaacccc gaccaaatcc ccaagcgcct tttgtgtaag gcctcgcgct aatctggctt
      661 ctttaagtct ttctgagagt gttgttttca tagttcaaat gtacaaccaa ggttttattc
      721 catcaaacga aaatggttgt tgactaaaaa caaccatagt tttaatcttg attcaaatta
      781 accacggagg ttgttatgaa cccagctatt aaaacagcga tcaatatcgt tggttcacaa
      841 aagaaactgg gcgctgcttg cgaagtttca cagcaggccg tctataagtg gcttcacaac
      901 aaagcaaagg tatcccctga acatgtcggc agcattgtta cggctactgg tggagtagtg
      961 aaggcacacc agattcgccc ggatcttccg aagttgtttc cacacaccga aaagaacgca
     1021 gcttaaattt ccatttcacg ctctttaaca ataagcaatc aacttaacag tcaattcaaa
     1081 ctaaaggagt caattatgca accacttaca taccaacaga ctagcggatt tagcccgact
     1141 gcggtgataa atcgttctca aacaaaacag gtgccaggcc acgaaaaaat ccgtgatgcc
     1201 gtccgcgcct ggtcggctga agataatcag gatgtcgttg ccacactcat tgtgaatgag
     1261 tatcgggagc agggcggcgg caccatcgat ttccctgatg atgtcagccg tgcacgccag
     1321 aagctgttcc gcttcctcga taacaaattc gattctgaaa aataccgaaa taacgtgcgt
     1381 gaactgaccc cggcaattct ggcggtacta ccgctggaat atcgcggtta cctggttgag
     1441 caggatagct ttatggctag gttggctgaa atggaaaagg aactcagtga ggcaaaacag
     1501 gctgtcattc tcaacgcacc acgccaccag aaactgaagg aaattagtga aggtattgtg
     1561 tcgatgtttc gtgtggaccc agatctggct ggtccactga tggcgatggt caccaccatg
     1621 ctgggggcaa tatgacaggt tcagaaatgg cgaaagccgg tctgctggaa cagaaccgac
     1681 tttcaggtgc aaatcgtaac gcactcattg cgggaggaat tatggcaaac actgctgaga
     1741 tattcaattt tccagtgccg gatgtggcac aaaaggagcc gcgcgtggca gatctcgatg
     1801 atggttatac gcgcattgca aatgagttgc tggaagctgt gatgctggcc ggattaacac
     1861 agcaccagct tctggtcttc ctggctgtca tgcgcaaaac atatggcttt aataaaaaac
     1921 tggattgggt gagcaacgag caactttccg aattgaccgg gatattgccg cacaagtgtt
     1981 ctgctgcaaa aagcgttctg gtaaagcgt
//
LOCUS       DBFIFM010000368         1942 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.370, whole genome shotgun sequence.
ACCESSION   DBFIFM010000368 DBFIFM010000000
VERSION     DBFIFM010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.370"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(181..1080)
                     /locus_tag="N7C74_004830"
     CDS             complement(181..1080)
                     /locus_tag="N7C74_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2434521.1"
                     /translation="MKIDLNLLPIFIAVAEESNFSKAAARLGVTRSAVSQGIRRLEDA
                     FGTMLVMRTTRSVNLTEAGERLHKSLSLPLSGIEAAFEEMVSDHVPRGLLRIAVTSIA
                     EEFLSGPLIASFAAANPAVTLDIVVTDEEFDIVAAGYDAGVRLGEVIEKDMIAVPLTG
                     RQREMVVASPSYLAANSTPVHPRELVNHKCIGWRQSPEVAPYRWPFEENGRAFDLAIE
                     PQITTNDLRLMLRLALAGGGITIATQETFRPYIESGKLVSLLDDFLPQFPGFYLYFPQ
                     RRNIAPKLRALIDHVKEWRQQLA"
     gene            1179..1901
                     /locus_tag="N7C74_004831"
     CDS             1179..1901
                     /locus_tag="N7C74_004831"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU2434522.1"
                     /translation="MDKVILITGASSGIGEGIARELGMTGAKVLLGARRVERIEAIAT
                     EICRAGGIAKARELDVTDRQSMADFVQAALDSWGRVDVLINNAGVMPLSPLAAGKQDE
                     WALTIDVNIKGVLWGIGAVLPVMEAQGSGQIINLGSIGALSVVPTGAVYCASKFAVRA
                     ISDGLRQESSKIRVTCVNPGVVESELASTITHVETMKAMDAYRSVALQPADIARAVRH
                     IIESPESVDTTEITIRPTASAN"
BASE COUNT          521 a          457 c          471 g          493 t
ORIGIN      
        1 tatgtattaa aatcattgtg ttggtggcga ttattggttc ctattatcgc accatttaaa
       61 tcaagtagtt accccatatt taaatacacc acgtttcctc ctgtgccgca tttgtgccat
      121 tgtaaccctg gcaattaatc aaaatactgt tctgacatca ggcaatgcag gtgcagacat
      181 ttaagccaat tgctgccgcc attccttaac gtggtcaatc agagcgcgga gctttggtgc
      241 aatattgcga cgctgtggga aatacagata gaagcccgga aattgtggaa gaaagtcatc
      301 aagcagagat acaagcttac cgctttcaat atacggcctg aaagtttcct gagtggcaat
      361 tgttattcct ccgccggcaa gagccagcct caacatcaga cgcagatcat tagtcgtaat
      421 ctgcggttca atcgcaaggt cgaaagctct cccgttttct tcaaatggcc agcgataagg
      481 cgcaacctcc ggggactgac gccagccgat acacttatga ttcacaagtt cacgcggatg
      541 aacaggtgtg ctgttggctg cgagatagga gggagaggca acgaccattt cacgctgcct
      601 gccggtaagg ggcacagcaa tcatgtcttt ttcaatgacc tctccgagcc ttacgccagc
      661 atcgtaacct gcggccacga tatcgaattc ctcatccgtg acaacaatat caagcgtaac
      721 ggctggattg gctgctgcaa acgaagcgat tagcggacct gaaagaaatt cttcagctat
      781 tgacgtcacc gcgattctga gaagtccgcg tggcacatgg tcagaaacca tctcttcgaa
      841 cgctgcctca ataccggata aaggtaatga cagcgattta tgcagccgtt ctcccgcttc
      901 tgtcaaatta actgagcgcg tggtccgcat tacaagcatt gtcccgaatg catcttcaag
      961 gcgtcttatt ccctgactga ccgctgaacg ggtaacgccc agccgtgcgg cggctttact
     1021 aaaattactc tcttctgcta ccgcgataaa aatgggtaaa agattaagat cgattttcat
     1081 tgtttaatgc cgctaaccac tgagtacatt gaaagcagga tactggaacc aatcataaag
     1141 ttctattctc tctccgaaca catttgaagg agaacgtaat ggataaggtc atattaatta
     1201 ccggcgcatc aagtggtatt ggggaaggta ttgccagaga gcttggaatg acaggtgcaa
     1261 aggttttact gggagcacgc agagttgagc gtatcgaagc cattgcaacc gaaatctgcc
     1321 gcgcaggagg aattgctaaa gcgcgggagt tggatgtcac agaccgacag tccatggccg
     1381 atttcgtgca agcagcgctg gatagctggg ggcgagttga tgttcttatc aataatgcgg
     1441 gcgttatgcc gctttcaccg cttgcagcag gaaaacaaga tgaatgggcg ctcactattg
     1501 acgtgaatat caaaggtgta ctgtggggaa ttggcgctgt acttccggtg atggaagcac
     1561 agggttccgg tcagataatc aatcttggtt ctattggtgc cctttctgtt gtgcccacag
     1621 gcgcagtcta ttgtgcatca aaatttgcag tacgggctat ttctgatggc ttacggcagg
     1681 aaagctcgaa aatccgtgtg acctgcgtaa atcctggcgt tgtcgaaagt gaactggctt
     1741 caaccattac ccatgtggaa accatgaagg caatggacgc ctaccgttct gttgcacttc
     1801 aacccgcgga tattgcccgt gctgtaaggc atattattga atctcccgaa agcgttgata
     1861 caaccgaaat taccatcaga cccactgcat ctgcaaactg aagaataact tttacattca
     1921 cgttttatta aataggtcag aa
//
LOCUS       DBFIFM010000369         1875 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.371, whole genome shotgun sequence.
ACCESSION   DBFIFM010000369 DBFIFM010000000
VERSION     DBFIFM010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.371"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..403
                     /locus_tag="N7C74_004832"
     CDS             <1..403
                     /locus_tag="N7C74_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HGU2434523.1"
                     /translation="HVKKIVEMLKLQQRTEYISHREVFRPWGKFDSIDQGERYKVKKI
                     IVKPGEGLSLRMHHHRSEHWIVLSGTAKVTLGDKTKLVTANESIYIPLGAAYSLENPG
                     IIPLNLIEVSSGDYLGEDDIIRQKERYKHED"
     gene            407..1777
                     /gene="cpsG"
                     /locus_tag="N7C74_004833"
                     /gene_synonym="manB"
     CDS             407..1777
                     /gene="cpsG"
                     /locus_tag="N7C74_004833"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HGU2434524.1"
                     /translation="MKSLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSEALKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGEMVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVKLMEKKTKAL
                     LKLLSE"
BASE COUNT          504 a          435 c          489 g          447 t
ORIGIN      
        1 gcatgtaaaa aaaatagtcg aaatgcttaa attgcagcaa cgtacagagt atattagtca
       61 tcgtgaagtt ttccgaccat ggggaaaatt tgattcgatt gaccaaggtg agcgatacaa
      121 agtcaagaaa attattgtga aacctggtga ggggctttct ttaaggatgc atcaccatcg
      181 ttctgaacat tggatcgtgc tttctggtac agcaaaagta acccttggcg ataaaactaa
      241 actagtcacc gcaaatgaat cgatatacat tccccttggc gcagcgtata gtcttgagaa
      301 tccgggcata atccctctta atcttattga agtcagttca ggggattatt tgggagagga
      361 tgatattata agacagaaag aacgttacaa acatgaagat taacatatga aatctttaac
      421 ctgctttaaa gcctatgata ttcgcgggaa attaggcgaa gaactgaatg aagatattgc
      481 ctggcgcatt gggcgtgcct atggcgaatt tctcaaaccg aaaaccattg ttttaggcgg
      541 tgatgtccgc ctcaccagcg aagcgttaaa actggcgctt gcgaaaggtt tacaggatgc
      601 gggcgtcgat gtgctggata tcggtatgtc cggcaccgaa gagatctatt tcgccacgtt
      661 ccatctcgga gtggatggcg gcatcgaagt taccgccagc cataacccga tggattacaa
      721 cggcatgaag ctggtgcgcg aaggggctcg cccgatcagc ggtgataccg gactgcgcga
      781 tgtccagcgt ctggcagaag ccaatgactt ccctcctgtc gatgaaacca aacgtggtcg
      841 ctatcagcaa atcaatctgc gtgacgctta cgttgatcac ctgttcggtt atatcaacgt
      901 caaaaacctc acgccgctca agctggtgat caactccggg aacggcgcag cgggtccggt
      961 ggtggacgcc attgaagccc gatttaaagc cctcggcgca ccggtggaat taatcaaagt
     1021 acacaacacg ccggacggca atttccccaa cggtattcct aacccgctgc tgccggaatg
     1081 ccgcgacgac acccgtaatg cggtcatcaa acacggcgcg gatatgggca ttgcctttga
     1141 tggcgatttt gaccgctgtt tcctgtttga cgaaaaaggg cagtttatcg agggctacta
     1201 cattgtcggc ctgctggcag aagcgttcct cgaaaaaaat cccggcgcga agatcatcca
     1261 cgatccacgt ctctcctgga acaccgttga tgtggtgact gccgcaggcg gcaccccggt
     1321 aatgtcgaaa accggacacg cctttattaa agaacgtatg cgcaaggaag acgccatcta
     1381 cggtggcgaa atgagcgctc accattactt ccgtgatttc gcttactgcg acagcggcat
     1441 gatcccgtgg ctgctggtcg ccgaactggt gtgcctgaaa ggaaaaacgc tgggcgaaat
     1501 ggtgcgcgac cggatggcgg cgtttccggc aagcggtgag atcaacagca aactggcgca
     1561 acccgttgag gcaattaatc gcgtggaaca gcattttagc cgcgaggcgc tggcggtgga
     1621 tcgcaccgat ggcatcagca tgacctttgc cgactggcgc tttaacctgc gctcctccaa
     1681 caccgaaccg gtggtgcggt tgaatgtgga atcacgcggt gatgtaaagc taatggaaaa
     1741 gaaaactaaa gctcttctta aattgctaag tgagtgatta tttacattaa tcattaagcg
     1801 tatttaagat tatattaaag taatgttatt gcggtatatg atgaatatgt gggctttttt
     1861 atgtataacg actat
//
LOCUS       DBFIFM010000370         1869 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.372, whole genome shotgun sequence.
ACCESSION   DBFIFM010000370 DBFIFM010000000
VERSION     DBFIFM010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.372"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..1144)
                     /locus_tag="N7C74_004834"
     CDS             complement(<1..1144)
                     /locus_tag="N7C74_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU2434525.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGDPQVGPSSKPDWFYVVVLAGQSNGMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADTGASQDSARWGVGKPLYQDLIARTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLTQFRADLSVFNAQCHGGSAADV
                     PWICGDTTYYWKNTYATQYDTVYGGYKNRESEGVYFVPFMTDGNGVNTATNAPAEDPD
                     IPASGYYGAASRTNGNQVSSNRPTHFSSWARRSIIPDRLATAILNAA"
     gene            1413..1529
                     /locus_tag="N7C74_004835"
                     /pseudo
     CDS             1413..1529
                     /locus_tag="N7C74_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1634..1710)
                     /locus_tag="N7C74_004836"
     tRNA            complement(1634..1710)
                     /locus_tag="N7C74_004836"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1674..1676),aa:Arg,seq:tct)
     gene            complement(1724..1800)
                     /locus_tag="N7C74_004837"
     tRNA            complement(1724..1800)
                     /locus_tag="N7C74_004837"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1764..1766),aa:Arg,seq:tcg)
BASE COUNT          420 a          529 c          486 g          434 t
ORIGIN      
        1 cggctgcgtt cagaatagcg gttgccagac gatccggaat aatgctcctg cgcgcccatg
       61 aactgaaatg tgtcgggcgg tttgatgata cctggtttcc attcgttctc gatgccgcac
      121 cgtaatatcc tgatgccgga atatccggat cttctgccgg cgcgttagtg gcggtattga
      181 cgccgttacc gtctgtcatg aagggcacaa aataaacgcc ctcactctcc ctgtttttat
      241 acccgccgta cacggtgtcg tactgggtag cgtatgtatt tttccagtaa tacgtcgtat
      301 caccacaaat ccacggcaca tctgcagcac tgccaccatg gcactgcgcg ttaaacacgg
      361 agaggtcagc acgaaactgt gtcagcatgg ctgtaaacag cgcaggttgc tgtgcgtggg
      421 tggcggcgct catgtcaaac tctccctgca tccagcacac cgccagcaac acatttttcg
      481 ggttcttctg taatgcagct ttagtgcgcg caatcaggtc ctgatataac ggtttaccca
      541 caccccagcg tgccgaatcc tggctggccc ccgtgtccgc actgaatgtc ccctcagcgc
      601 cctgggtgaa tgcagaacca ccacgacagc atggtaccag caggatcccc gcgttattcg
      661 ggatatacgg aagcagtttt ttggcaatat gtaagccctg gccgacacag ccgtactgcc
      721 ctttgctcag gtctgccttc ggatgattca gcgtactcat atcctgcaca tcatgcaggc
      781 agtggtcggc cggaatgatg tcgttatacg tacaactctc tccacccgga gttaccgtgc
      841 tgcggcgcgc cagctgttta atgcgcggat ccggagcatc gtaagaatcc ggtaacggaa
      901 gcccttcacc gtaggccatg ccgttggact gtccggcaag cacaaccacg tagaaccagt
      961 ccggcttaga tgaagggccg acctgtggat ctccttcaat agccaccgcc tgcatcagtg
     1021 tgtacggcgt aatggcaacc ggtccgccgt atggctgcca gccctctttc agtttgtgtg
     1081 tcagcttttc cgcaaggtct gacggcgacg ccgccctgac aacatcatag tgtttaaatg
     1141 ccatgaatcc tcccggccgg gataatattg tgagtaaaat gaggagcggg ctgaagtccg
     1201 gaagttacag gacaatggca gaagagagac aacagcccgc aatacgaaaa aggccgcgct
     1261 attgcgcaga gtgattactg tcggatatta ttcgccagct gaaatattac ttcacgtttt
     1321 gttgtttatt ccttgccgcc cgcgtctccc tgcgcgggct ttttttgtcc ataagaaagc
     1381 ccctccggag aggggctgga gagtggcgct atgtgccatt gcatggtgcc gggtgcctcc
     1441 cggtgaattc agtaccagca cctgaatccg cgattatccc atatacctac tcgctgattg
     1501 cccctccgca cagggggatt caccatgcca gtttctttta acaaactccc cgcaaaccag
     1561 acaacagtca accgcctgaa ttgtgagaca tttaaaaaaa aggcccgcaa aagcgagcca
     1621 gggaaaataa gtgtggcgcg ttgtactgga ttcgaaccag tgaccgattg cttagaaggc
     1681 aattgctctg tccggctgag ctaacaacgc aggatacaga taatggaccg ccttcgggga
     1741 cccgaactcc gcgcaaccag cttcgaaagc tggcgctctt tcctgatgag ctaatggcgg
     1801 tatgtgatgg tggcccttgc tggatttgaa ccagcgacct ggcgattatg agtcgctcgc
     1861 tctcaccac
//
LOCUS       DBFIFM010000371         1866 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.373, whole genome shotgun sequence.
ACCESSION   DBFIFM010000371 DBFIFM010000000
VERSION     DBFIFM010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.373"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..132
                     /locus_tag="N7C74_004838"
     CDS             <1..132
                     /locus_tag="N7C74_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU2434526.1"
                     /translation="SRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
     gene            83..784
                     /gene="traT"
                     /locus_tag="N7C74_004839"
     CDS             83..784
                     /gene="traT"
                     /locus_tag="N7C74_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HGU2434527.1"
                     /translation="MSPRQPSGKNIIRWLLKKRTNGSVRYCQYTSTIWLEPASERTVF
                     LQIKNTSDKDMSGLQGKIADAVKARGYQIMTSPDKAYYWIQANVLKADKMDLRESQGW
                     LSRGYEGAAVGAALGGGITAYNTGSAGTTLGVGLATGLIGMAADAMVEDINYTMITDV
                     QIAERTRTSVRTDNVAALRQGTSGSKIQTSTETGNQHKYQTRVVSSANQVNLKFEEAK
                     PHLEDQLAKSIANIL"
     gene            complement(832..1083)
                     /locus_tag="N7C74_004840"
     CDS             complement(832..1083)
                     /locus_tag="N7C74_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HGU2434528.1"
                     /translation="MESFVQDSPFYSGRDLYWLRPKVELTLEEKLYYCSCIRRNRHKY
                     SYGRQANRTLKNLLVPSLDSVPAWVYGVTGKIISELSER"
     gene            complement(1107..1397)
                     /locus_tag="N7C74_004841"
     CDS             complement(1107..1397)
                     /locus_tag="N7C74_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HGU2434529.1"
                     /translation="MNLSASMNDAGDGFELLMSGNMQDFNICCRRDNHYPEHKLIPLS
                     ELFDVNYGLNLELNKLEKDSSGINFVSRTSKNNGVSARVKLKDGISPCQQLC"
BASE COUNT          560 a          447 c          398 g          461 t
ORIGIN      
        1 agccgggatg aggccatctc agtaatacgg gaatacattg agattttcta caatcgtcag
       61 cgtcgtcact ctcgtctggg gaatatctcc ccggcagcct tcagggaaaa atatcatcag
      121 atggctgctt aaaaaaagaa caaatggtag tgtccgctat tgtcagtaca cctcaaccat
      181 ctggcttgaa cctgcgagcg aacgcaccgt tttcctgcag atcaaaaaca cgtcggataa
      241 agacatgagt ggactacagg gaaaaattgc cgatgcagtc aaagccagag gttatcagat
      301 catgacttcc ccggataaag cttactactg gatccaggct aacgttctca aagcagacaa
      361 aatggatctg cgtgaatctc agggatggct gagtcgcggt tatgaaggtg cagcagtcgg
      421 agctgctctt ggaggtggaa tcacagcgta caataccggg tcggcaggta ccactctcgg
      481 tgttggactg gctaccggtc tgattggcat ggccgcggat gcaatggtgg aagatataaa
      541 ttacacaatg attacagatg ttcagattgc cgagcgaacc agaaccagcg tcagaacgga
      601 taatgttgca gctttgcgtc agggaacatc aggctccaaa attcagacaa gcacagaaac
      661 cggcaaccag cataaatacc agacccgggt tgtatccagt gccaaccagg ttaatctgaa
      721 gtttgaagaa gctaaacctc atctggaaga ccagctggca aaatcaatcg ccaatatact
      781 ctgatataaa tatcccggtg gccattcacc tggcctccgg ccctcttagt ctcaacgttc
      841 agacaattca gatattattt tccctgtcac accatatacc catgccggaa cagaatctaa
      901 tgacggtacc agtaaattct tcagcgttcg gtttgcctga cgcccgtagc tgtatttgtg
      961 tctgtttctt cttatacaag agcagtagta caatttctct tccagagtca gttcaacttt
     1021 aggacgtaac caataaagat ctcttccact ataaaatggt gaatcctgta caaacgattc
     1081 cataacagaa cctcctgctg ctacagttaa cacagctgct ggcatggact aataccatct
     1141 tttaatttaa ccctggcgga gacgccatta tttttggacg ttcttgaaac aaaattaatt
     1201 cctgaagaat ctttctccag tttattcagc tcaagattca gtccataatt aacgtcaaat
     1261 aactctgata aaggtatcag tttatgttca ggataatgat tatcccgacg acaacaaata
     1321 ttaaaatcct gcatattacc cgacatcagg agttcaaaac cgtccccggc atcattcata
     1381 ctggcagata aattcattac ccagtttagt gctaatcaac aggtacggct taacttacag
     1441 gaattccccg ggaggggcgg actcatcctc agttagcggc aaaatcgcat cacagccaat
     1501 cgcctgtctc gattccccat cggcgaacaa tgtatgtggc cacaaaatcc tggctggctc
     1561 agaacgaaga gcatctgagc aaagaggtca tcaatctggt atgaatatct ggcagaaaat
     1621 atcccctgaa tccacaactg ctgtgcatat ctgctcaaat cagcatcaat cataaaccag
     1681 taatcacagt gcctgtgatt gggttacagg aataagtccc ccttcataag gcatatcatc
     1741 aataccggtg aaggaaaaga aagcatcaga tggcctgata gtctgcgtca gtcaatgaca
     1801 tcagtgcttt atccggaaca atgtatccat gttggcgaca gagaaagcca ttgccatgaa
     1861 cagttt
//
LOCUS       DBFIFM010000372         1835 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.374, whole genome shotgun sequence.
ACCESSION   DBFIFM010000372 DBFIFM010000000
VERSION     DBFIFM010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.374"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..1108)
                     /locus_tag="N7C74_004842"
     CDS             complement(<1..1108)
                     /locus_tag="N7C74_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU2434530.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGEPQVGPSSEPDWYYVIVLAGQSNAMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSESTGASQDSARWGVGKPLYQDLISRTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLTQFRADLSVFNAQCHGGSAADV
                     PWICGDTTYYWKNTYATQYDTVYGGYKNRESEGVYFVPFMTDGNGVNTATNAPAEDPD
                     IPASGYYGAASRTNGNQVSSNRPTHFSSWARRSII"
     gene            1085..1227
                     /locus_tag="N7C74_004843"
                     /pseudo
     CDS             1085..1227
                     /locus_tag="N7C74_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1422..1589)
                     /locus_tag="N7C74_004844"
     CDS             complement(1422..1589)
                     /locus_tag="N7C74_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HGU2434531.1"
                     /translation="MTVKLRLTVAALLLFLVVMVDFTSRIMSVLADGVLVCGIMVLLW
                     PVIKRNSLHNA"
     gene            complement(1586..>1835)
                     /locus_tag="N7C74_004845"
     CDS             complement(1586..>1835)
                     /locus_tag="N7C74_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HGU2434532.1"
                     /translation="AFTSEINAISATIIGQLDTNFKTGRRAALREIEDVKHDTREAED
                     VLDVAVAIAEADGEIEPEERKVLEEIAGVLGLRLENYL"
BASE COUNT          437 a          540 c          442 g          416 t
ORIGIN      
        1 gaataatgct cctgcgcgcc catgaactga aatgtgtcgg gcggtttgat gatacctggt
       61 ttccattcgt tctcgatgcc gcaccgtaat atcctgatgc cggaatatcc ggatcttctg
      121 ccggcgcgtt agtggcggta ttgacgccgt taccgtctgt catgaagggc acaaaataaa
      181 cgccctcact ctccctgttt ttatacccgc cgtacacggt gtcgtactgg gtagcgtatg
      241 tatttttcca gtaatacgtc gtgtcaccac aaatccacgg cacatctgca gcgctgccac
      301 catggcactg cgcgttaaac acggagaggt cagcacgaaa ctgtgtcagc atggctgtaa
      361 acagcgcagg ttgctgtgcg tgggtggcgg cgctcatgtc aaactcaccc tgcatccagc
      421 agacggccag cagaacgttt ttgggatttt tctgcaatgc cgcttttgtg cgggaaatca
      481 gatcctgata taacggcttg cccacccccc agcgtgccga atcctgactg gcccccgtgg
      541 actcgctgaa tgtcccctcc gcgccctggg taaatgccga accaccacga cagcatggta
      601 ccagcaggat ccccgcgtta ttcgggatat acgggagcag ttttttggca atatgtaaac
      661 cctggccgac acagccgtac tgccctttgc tcaggtcagc cctcggatga ttcagcgtac
      721 tcatatcctg cacatcatgc agacagtggt cagccggaat aatatcgtta tatctgcagg
      781 cagccccgcc cggcgtcact gtactgcggc gcgccagctg tttaatgcgc ggatccggag
      841 catcgtatga atccggcagc ggaagccctt caccgtaagc catggcattg gactgcccgg
      901 ccagtacgat gacgtagtac caatccggct cagatgaagg gccgacctgt ggctctcctt
      961 caatagccac cgcctgcatc agtgtgtacg gcgtaatggc aaccggtccg ccgtatggct
     1021 gccagccctc tttcagtttg tgtgtcagct tttccgcaag gtctgacggc gacgccgccc
     1081 tgacaacatc ataatgttta atcgacatcg aatttctccc gtgtagagga acagagttaa
     1141 aaagccggaa gcggaatcaa atcacaggat gaccatctgc cagtggctgg tcgtaaaaaa
     1201 aggccgcgcc atgcgcagcc gaaaataaag ggataacgat gatagtttga gaaaaacaga
     1261 aataacactt ttgtggcaaa gcatggtgcc gggtgcctcc cggtgaattc agtatcagca
     1321 cctgaatccg cgattacccc atattccttc ttgctgattg ccccaccgca cagggggatt
     1381 caccatgcag aagtgttttt aataaacagc aaacaaaaaa atcaagcatt atgcaggctg
     1441 tttcttttta tcaccggcca cagcaatacc ataatgccgc agaccagcac cccatccgcc
     1501 agcaccgaca tgattctgct ggtgaaatcc accatcacca ccagaaacag caggagtgca
     1561 gccacagtca ggcgcagttt taccgtcaca ggtaattctc cagacgaaga cccagaacac
     1621 cggcaatctc ttccagcacc ttgcgctctt ccggctcaat ttcgccgtct gcctccgcaa
     1681 tggccaccgc cacatccagc acatcttccg cttcacgcgt atcgtgtttc acatcctcga
     1741 tctcacgtaa cgccgcacga cgaccagttt taaagttcgt atccagctga ccgataatgg
     1801 ttgcgctaat cgcattaatt tctgacgtaa acgcg
//
LOCUS       DBFIFM010000373         1834 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.375, whole genome shotgun sequence.
ACCESSION   DBFIFM010000373 DBFIFM010000000
VERSION     DBFIFM010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.375"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            144..1286
                     /locus_tag="N7C74_004846"
     CDS             144..1286
                     /locus_tag="N7C74_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HGU2434533.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGIELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESATGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISTGLPLVAIGKGLVINPEWV
                     TLAESGRGHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD"
     gene            1760..1834
                     /gene="ykgR"
                     /locus_tag="N7C74_004847"
                     /pseudo
     CDS             1760..1834
                     /gene="ykgR"
                     /locus_tag="N7C74_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
BASE COUNT          580 a          389 c          383 g          482 t
ORIGIN      
        1 aaaaacaaaa aagcagcaac tcaattatta gaaacccttt aaagggatta taaataaagc
       61 cagaactctt ggttccaaag ctggcaacaa ctaaccagtt aaaacatacg catcaaaatc
      121 agataaacat ttgaggtatt gttatgacaa ataaacaccc atcattgttt tctcctttta
      181 tgctgacaga aaaaataaag ctacgtaatc gtatcgttat ggctcctatg acgacctggt
      241 cagcgaatcc tgatggaacc atctctgagc aagaactcga attttataaa cgtcgctcac
      301 aaaatgttgg tttagtaata acaggatgta cctatgtcac gccaagcggc attgggttta
      361 ctcatgaatt tgccgcgtat gacgaccgtt tcattaacag ccttgaaaag ttagcagcgg
      421 cagccaaaag tggcggtgct ccggctattt tgcagatttt ccacgccggt aataaagcca
      481 tcccggagct tgtaccaaat aacgatgtta ttagtgccag cgcatcaagc gtaaaatctg
      541 gtgactttat gaagagagtg gtacaaagca gggaaatgac tgaaaacgaa attcaggaaa
      601 ccattagagc attcggagat gtcacaaaaa gggcgattaa agcaggtttt gatggcattg
      661 aacttcatgg tgcgcacgga ttccttctac agaatttttt ctcccctcta tttaaccagc
      721 gtaatgaccg atggggagga gatctcgaag gacgtatgcg cttcccgctg gccgtattac
      781 aggaagtaaa aaatgtagtc tatgaatatg cgacaaagcc ctttgccatc ggttacagaa
      841 tatctcctga agaatctgca acaggggggc tgcggataga ggatacttat aaactccttg
      901 atcggctcat ttcatccggt atttcgtata tacatacctc gctggtcagt attaacgata
      961 gctaccctgt tgagtcgcca aatggccccc gaactattga attgatttta aatcatattg
     1021 caggcagagt gccggtaatc gctgcaggta aaatacgcac accgtctcag gctcaagaag
     1081 caatttccac tggcctgcca cttgttgcga tcggtaaagg gctggtgata aatccagaat
     1141 gggtgacttt agctgaatca ggtagaggcc atgaaattca aacaaccctt aatccacaac
     1201 ttgttcctga attaaccatt ccggataaac tatgggatca aattcaggca tcgaaaggaa
     1261 ctggctggtt tccgttaatg gattaaatat ctattactct cccgagagta aaactatcaa
     1321 atcaatgagt aaccgataca gcctcacaaa ttctttcaga taattaaccc gtcactgcgg
     1381 cacgttctct cataaggcgt gcctgcagtg taatctagta tgtatccggc atagttgacg
     1441 gtttaataga gctcaattcc tcgcccaggt taccaacaac gatatcttgt ataacacccc
     1501 aggagatacg acattatgat gagatgcgat atgctcctca gcgccaatca acgccaggac
     1561 tgggccagtc ttgagatcag actcattccc ggaactgact tcaggcgaca tatatcaatg
     1621 acgttggggc aataaaagat gcctgatgta atacatctat tcaaatttgt gatacatgtc
     1681 aaaaaattgg ttgaccaaac tcgttattta tataagggca cttacgaagt gcgctctttt
     1741 ttaaagcgag gaagtaccaa tgaaagagaa taaagtacaa caaatcagtc ataaactgat
     1801 taatatcgtt gttttgtcgc aattgtagaa tacg
//
LOCUS       DBFIFM010000374         1829 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.376, whole genome shotgun sequence.
ACCESSION   DBFIFM010000374 DBFIFM010000000
VERSION     DBFIFM010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.376"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..95)
                     /locus_tag="N7C74_004848"
     CDS             complement(<1..95)
                     /locus_tag="N7C74_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434534.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDNNSNS"
     gene            complement(79..>1829)
                     /locus_tag="N7C74_004849"
     CDS             complement(79..>1829)
                     /locus_tag="N7C74_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU2434535.1"
                     /translation="HRETARSFGGAGSTAGYEQVTAYTLTGQLQSRHLNLPQLDCDYD
                     WNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPYATDPAGNRLPDPELH
                     PDSTLTAWPDNRIAEDAHYVYRYDEYGRLTEKTDRIPEGVIRMHDERTHHYHYDNQHR
                     LVFYTRIQYGEPLVESRYLYDPLGRRTGKRVWRRERDLTGWMSLSRKPEVTWYGWDGD
                     RLTTVQTGTTRIQTVYRPGSFTPLIRIETENGEREKAQRRSLAEKLQQEGSEDGHGVV
                     FPAELVRMLDRLEEEIRADRVSSESRAWLAQCGLTVEQLEKQVEPEYTPARTLHLYHC
                     DHRGLPLALISEDGNTAWSAEYDEWGNQLNEENPHHLHQPYRLPGQQHDEESGLYYNR
                     HRYYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQVIDPMGLDAIENMTSGGLIYAVSG
                     VPGLIVANSITNSAYQFGYDMDAIVGGAHNGAADAMRYCYLMCRMTKTFGSTIADVIG
                     KNHEAAGDRQGQPAKERIMDLKNNTVGIACGDFSAKCSDACIEKYNIGQLFGLDGIKA
                     DNPIKAKQGSSDASNY"
BASE COUNT          348 a          566 c          447 g          468 t
ORIGIN      
        1 tcagaatttg aattgttatc aaacccaagg taaacaacaa ataaacaaag ggtaattaaa
       61 aaaatgacaa gtttgcctct aataatttga agcatctgaa ctcccttgct ttgcttttat
      121 tggattatct gcttttatac catctaaccc gaagagttgc ccaatgttat atttttcaat
      181 acatgcatcg ctacatttgg cagaaaaatc gccacaagca ataccgacag tgttattttt
      241 aagatccatg attctttctt tagctggctg accttgtcta tccccagccg cctcatgatt
      301 tttacctatc acgtcagcta ttgttgatcc aaatgtctta gtcattcgac acatcaagta
      361 acaatatctc attgcatcgg cggccccatt atgagctccg ccaacaatag catccatatc
      421 ataaccgaac tggtaagcac tgttagtaat gctgtttgca acaatcaatc caggtacacc
      481 agatacggca taaattagtc cacctgatgt catattctca atcgcatcta accccattgg
      541 gtctatcact tgtatgggat tcaacggata ctgatacata ttccatccac ctctcaaccc
      601 aatcgggtcc ggggtgatat accgcccctg caacggatcg tagtaccggt gacggttata
      661 gtacagcccc gactcctcat catgctgctg gcccggcaga cggtacggct ggtgcaggtg
      721 atgcgggttc tcctcattaa gctggttgcc ccattcatca tattccgcgc tccacgccgt
      781 attgccgtct tcgctgataa gcgccagcgg cagtccccgg tggtcacagt ggtacagatg
      841 cagcgtgcgc gccggcgtgt attccggctc cacctgtttt tccagttgct ccaccgtcag
      901 tccgcactgc gcaagccacg cccggctttc actgctcacg cggtctgccc ggatttcttc
      961 ctccagcctg tccagcatcc tcaccagttc tgccgggaac accacaccgt gaccgtcctc
     1021 actcccttcc tgctggagtt tctccgccag gctgcggcgc tgcgctttct cccgctcgcc
     1081 gttctccgtt tcgatgcgga tgagcggtgt gaagctcccc ggccggtata ccgtctggat
     1141 acgtgtggtg ccggtctgta ccgtcgtcag cctgtcgccg tcccacccgt accaggtcac
     1201 ctccggttta cgcgacagcg acatccaccc cgtcaggtca cgctcccgcc gccacacccg
     1261 tttccccgtc cggcggccca gcgggtcata gaggtagcgg ctctcgacca gcggctcgcc
     1321 gtattgtatc cgcgtgtaga acaccaggcg gtgctggttg tcgtaatggt agtggtgggt
     1381 ccgctcgtcg tgcatccgga taaccccttc cggaatgcgg tccgtcttct ccgtcagcct
     1441 gccgtattca tcgtagcggt agacatagtg cgcatcttcc gcgatgcggt tatccggcca
     1501 cgccgtgagc gtgctgtccg gatgcagttc cgggtccggc agccggtttc ctgccgggtc
     1561 cgttgcatac gggatatcga tatccaggtt cgctgccgtg gtgtggacgc ccgtcagcct
     1621 tcccgtgtcg ctgtaacggt actcccggct ctcctgcggg ccgctgatgc ggattagctg
     1681 tccgttgtcg ttccagtcgt agtcacagtc aagctgcggg aggttcaggt gcctgctctg
     1741 tagctgccct gtgagggtgt acgccgtgac ctgttcgtac cccgctgtac tgcccgcccc
     1801 gccgaagctg cgggccgtct cacggtgca
//
LOCUS       DBFIFM010000375         1812 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.377, whole genome shotgun sequence.
ACCESSION   DBFIFM010000375 DBFIFM010000000
VERSION     DBFIFM010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.377"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            139..1431
                     /gene="sctN"
                     /locus_tag="N7C74_004850"
     CDS             139..1431
                     /gene="sctN"
                     /locus_tag="N7C74_004850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
                     /protein_id="HGU2434536.1"
                     /translation="MKKLKLLNKYSYLHSINGSLIEAELDDVSVGEVCEIYASRQANE
                     RIARAQVVGFRNGKTLLNLIGSSVGLTRTAVLKPTGEQLTIQISDAFLGSVLNASGQI
                     MERFVPDTPGDRGNLRLIDELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFA
                     SAGCGKTVLMHMLVNNTEADVFVIGLIGERGREVTECAESLKKSVNAAKCVLVYATSD
                     FSSVDRCNAALMATTVAEYFRDRGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYP
                     ASVFDSLPRLLERPGPTLKGSITEFYTVLLEGEDESDPLGDEIRSILDGHIYLSRKLA
                     GQGHYPAIDVLKSVSRVFGQVTDEKHRDNAARVRKNLTTLEDLQVFIDLGEYRAGQNA
                     ENDFAMNARPKLTNWLKQSVNEKMPMSETLKELERIVK"
     gene            1421..>1812
                     /locus_tag="N7C74_004851"
     CDS             1421..>1812
                     /locus_tag="N7C74_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HGU2434537.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLAEKERLYDMQL
                     KNLQSLLDMKELLGEVVFRQDIFYSLRKVTVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYDRLKKRIKKQLL"
BASE COUNT          577 a          334 c          438 g          463 t
ORIGIN      
        1 gtcgttttat caaaaaactt attgaatcta gttatcgaga cctcgaagta ctttcgtacg
       61 gggaactaac ggaaaatgtt cccgttaata ttctgaaaac tatttaggat aatgtcttgc
      121 gatatggagc atgctggtat gaaaaaatta aaattattaa ataaatacag ctacttacat
      181 agcataaacg gctctctgat tgaagcagaa cttgacgacg tttctgtggg tgaagtatgc
      241 gaaatttatg cgagcaggca agcaaatgaa cgtatagcca gggctcaggt cgttggcttt
      301 cgcaatggga agacattatt aaaccttata ggcagctctg tggggcttac acgtactgca
      361 gttttgaaac caacgggaga gcagttaacc atacagatca gtgatgcctt tcttggttcg
      421 gttttgaatg cctctggtca aattatggaa aggtttgtcc cggacactcc aggcgatagg
      481 ggaaatttac gcctgattga tgaactgcca ccgtcgtatc aggaacgtcg agttattaat
      541 acaccacttg agacacggat aagagtcatt gatggcgtac tgacatgtgg cattggtcaa
      601 cgtgttggga ttttcgcctc ggctggctgt ggcaaaacgg ttttaatgca tatgctcgta
      661 aacaatactg aagctgatgt gtttgttatt ggcttgattg gcgaacgtgg tagggaagtt
      721 acggaatgcg cggaatcgct caaaaaatca gtaaatgcag cgaaatgcgt attggtctat
      781 gccacctctg atttttcgtc agtagaccgg tgtaatgccg ccctgatggc gacaaccgtg
      841 gcagagtatt ttcgagatcg aggaaagaga gttgtacttt ttatcgactc aatgacgcgg
      901 tatgcacgag cactacgtga tatgaaactg gcagcgggtg aaccacctgc cagacgtggt
      961 tatcctgctt ctgtttttga cagtttgcct cgcttactgg agaggccggg accaacacta
     1021 aaaggaagta ttaccgagtt ttataccgtg ttactggaag gggaagatga atcagaccca
     1081 ctgggtgacg aaattcgctc aatccttgat ggccatatct atctaagtcg caaactcgcg
     1141 ggtcaaggtc actatccggc aatagatgtt ctaaaaagtg tgagtcgagt atttggtcag
     1201 gtcactgatg aaaaacacag agataatgct gcacgggtaa ggaagaacct cacaactttg
     1261 gaagatctac aggtgtttat tgacttaggg gaatatcgcg cagggcaaaa tgctgaaaat
     1321 gattttgcta tgaatgcccg gcctaaactc acaaattggt tgaaacagtc agttaatgaa
     1381 aaaatgccaa tgagtgaaac gctaaaagag cttgaacgaa ttgttaagta aagttaatcg
     1441 acttatcaga agaacagcgc agagcctggc tgcatgtgaa gcatcattac aaaagttaaa
     1501 tgcagaaaaa gaaaagttag ccgaaaagga acgcctgtat gatatgcaac ttaaaaacct
     1561 gcagtcactg ttagatatga aggagttact cggcgaagtt gtatttcgcc aggatatatt
     1621 ttactcgtta cgtaaagtta cagtcattca gcagcagatc gctgagataa atcttgaaaa
     1681 gcaaaaaata gccgagcggc gaaaaatact taacaaggaa atagttcaac agcaagcaca
     1741 aagaaaacac tggtggctaa aaggagaaaa atacgaccga ctaaaaaagc gcatcaaaaa
     1801 acaactttta aa
//
LOCUS       DBFIFM010000376         1795 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.378, whole genome shotgun sequence.
ACCESSION   DBFIFM010000376 DBFIFM010000000
VERSION     DBFIFM010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.378"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            6..140
                     /locus_tag="N7C74_004852"
     CDS             6..140
                     /locus_tag="N7C74_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HGU2434538.1"
                     /translation="MKLILGTACKTRHLVCKNVLRLAGELSIVRVLNDFQPLLILLVN
                     "
     gene            complement(137..379)
                     /locus_tag="N7C74_004853"
     CDS             complement(137..379)
                     /locus_tag="N7C74_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGU2434539.1"
                     /translation="MPHRYPAAKINKMPKGSVPALQQEMLRRVSKRYDDVEVIIKSTS
                     NDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
     gene            511..1794
                     /gene="ydfJ"
                     /locus_tag="N7C74_004854"
                     /pseudo
     CDS             511..1794
                     /gene="ydfJ"
                     /locus_tag="N7C74_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
BASE COUNT          465 a          374 c          441 g          515 t
ORIGIN      
        1 gtaaaatgaa attaattctc ggtacagcat gtaaaacgcg gcacctcgta tgcaagaacg
       61 tgctgcggtt ggctggtgaa ctttcgatag tgcgagtatt gaatgatttc cagccgttac
      121 tgattttact cgttaattag cgaacaaacc actcgtcagc agactcccag gtatctttca
      181 gcgtctcctg aacaaaagtt tttgcagaat ctttatcggc ggtgcgagta actgaaaggc
      241 catcgttgct ggtggatttg atgatcactt ctacatcgtc ataacgttta ctgacacgtc
      301 gcagcatttc ttgttgcagg gcaggaacag aaccttttgg cattttgttg attttggctg
      361 cggggtatct gtggggcata tatgggacat aaaaaggcct caaataaaca gcaaaatcga
      421 gattagggat ttttagaaaa aatacaacca tctgaaaaaa cttagaaaac acccaaaaac
      481 ccacagaata gtaaaaaaca tctatgaatt atggatttcc agttatattc gctcggcgca
      541 gcgttagtgt ttcatgaaat attttttcct gaatcatcaa cggcaatggc gttaattctg
      601 gcaatgggaa cctacggtgc aggttatgtg gcgcgtattg tcggagcatt tattttcggc
      661 aaaatgggcg acagaatagg gcgtaaaaaa gtgctcttta ttaccatcac catgatgggg
      721 atctgtacca ccttaattgg agtgttaccg acctatgcac agattggtgt ttttgccccc
      781 atcttgctgg tgacgctgcg tattattcag ggattgggtg caggtgcgga aatttccggt
      841 gccggtacga tgctggcgga atatgcgcca aaaggtaagc gcggaattat ctcctcattt
      901 gtggctatgg gaactaactg cggaaccttg agcgcaacgg caatctgggc ctttatgttc
      961 ttcattctca gtaaagagga actgctggcg tggggatggc gtataccgtt cctggcgagc
     1021 gttgtcgtga tggtctttgc tatctggttg cgtatgaatc tgaaagaaag cccggtcttt
     1081 gagaaggtta acgacagcaa ccaaccgaca gcaaaacctg cacctgctgg tagcatattc
     1141 cagagcaaat ccttctggct ggcaacaggg ctgcgttttg gtcaggcagg taactccggg
     1201 ttaattcaga ctttccttgc aggctattta gtacagacgt tattgtttaa caaagcaatt
     1261 ccaacagatg cattgatgat cagttcgatt cccggcttta tgaccattcc gttccttggt
     1321 tggttatccg ataaaattgg tcgccggatc ccgtatatta ttatgaatac ctccgcgatt
     1381 gtgctggcat ggccaatgct ttctatcatc gtagataaaa gctatgcccc gagcaccatt
     1441 atggttgcac tgattgtgat tcataactgt gcggtgctgg gattatttgc tctggaaaac
     1501 attaccatgg cagaaatgtt cggctgtaaa aaccgcttta cccggatggc tatttctaaa
     1561 gaaattggtg gtcttatcgc ttccggtttt ggtcctatcc tggcgggtat tttctgcacc
     1621 atgacggaat cctggtatcc gatcgccatt atgatcatgg catattcagt gattggttta
     1681 atctctgcgc tgaaaatgcc agaagtgaaa gaccgtgatt taagtgcgct ggaagacgct
     1741 gcggaagatc aaccgcgtgt tgtaagagct gcgcaacctt ccagaagtct gtaaa
//
LOCUS       DBFIFM010000377         1779 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.379, whole genome shotgun sequence.
ACCESSION   DBFIFM010000377 DBFIFM010000000
VERSION     DBFIFM010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.379"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            63..1448
                     /locus_tag="N7C74_004855"
     CDS             63..1448
                     /locus_tag="N7C74_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HGU2434540.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     ISIACQNQIDVCRPGNLHGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALADEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNKPPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTISVMIQPDVYHKGFCTRKKEIVKTSGHHARFLMCQPTSTQGTRIMIN
                     ELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGPLRHCREYAAKNAEYMA
                     RLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYVRLSNPLTFDNSALTVPVRLIPE
                     ELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWLLDLLYEQNRVVPVIEG
                     KTLCVAPNFDL"
BASE COUNT          532 a          316 c          425 g          506 t
ORIGIN      
        1 ttcgctattg tagcgggatt tgctgctatt ttactaaccc attaatgaaa agagaggctt
       61 ttatgtgttt acttgcacca gaaaatcctt atccgatata tgcactacca ccgctggtga
      121 gaaatgcaat aattgaaact caaaaaaata cacaggcacc tttggctatg gtggcaacat
      181 ccgcattaac agcgatctca attgcctgtc agaatcagat tgacgtgtgc agacctggaa
      241 atttacatgg gcctgttaat ctttactctc tgattctggc tgattccggt gaaaggaaaa
      301 cgaccgtgga taaagtgttt atgaaagcat tttatctcag ggatgaagcc ctggcggatg
      361 aatacgcgaa actggttgag aattacagta cagaaaagga aatatgggag caaaaacaaa
      421 aagcgctgga atcaaaattt cataaagaga ttcgtgccgg taaagattac aaggcaacag
      481 aatcagagct tgaaacgcat ctgaataaac ctcctgttcc gccgcagata cgtcgaacaa
      541 tttttaatga gacaacgata gagggaatgt taaaatatta ctccgatagc aatcgctctt
      601 ttgctcttgt atccagtgaa gggggggtaa tttttgacag ccgggccatg agtaaactgg
      661 gaattattaa cactttgtgg gatggaggtt ctcttttcat cgacaggaaa tcatctcccg
      721 gaattaattt gaaggagcca agactgacga tatcggtgat gattcagcct gatgtttacc
      781 acaaaggttt ttgtacgcga aaaaaagaaa ttgtgaaaac gtcaggacat catgcaaggt
      841 ttttgatgtg tcaaccaaca tcaacgcagg ggacaaggat aatgatcaat gagcttattg
      901 atgaaagtct ggcgatgagt ggtgaacgac gttgccttca cttttctcct caggctgcca
      961 gaatctggac ggattattac aatgatgtgg aatcgaagct ggggggattg gggcctttaa
     1021 gacattgccg ggaatatgct gctaaaaatg cagagtatat ggcaagactg gcaggactta
     1081 tttaccattc gagcggtgaa gagggggaga tttcccctta cactgcagaa atggcgagag
     1141 aattagcaat atggtacggt aatgagtatg tgcggttgtc taatccgtta acttttgaca
     1201 actctgccct gaccgtacct gtgcgactca ttccagagga gcttgaactt ttcaactgga
     1261 taaaaagcta ttgtattgag aaggggatcc tctgtatgaa aaaaaatgac attttacagc
     1321 gtggtccgaa ccgtttccgg aaaaaggata aaatcaactg gttactggat ttactgtatg
     1381 aacaaaacag agttgtaccg gttattgagg gaaaaacgtt gtgtgtcgca cctaactttg
     1441 acctctgaac gacttaatcg ttgagtacat atagtctcct ctgctacttg cggaggagac
     1501 taatgtcttt accatgtcag cgttgtatta gaaaactctg gtacaggtca gtcagtaaat
     1561 ataactggtg taaccactat ttactattgg atagagtgtt gctcaggata ttgtcggctt
     1621 tagtctgagg tgaggtgggg ccttcattgt tttcgtgttc aataaataat ttaggttaat
     1681 tagtattatt agtggaagtt atgtgatctg ttatgttgtc ttgtacgcac ttaacactgg
     1741 ttacgtaccg gaacactatt agagtatgtt atggctctg
//
LOCUS       DBFIFM010000378         1778 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.380, whole genome shotgun sequence.
ACCESSION   DBFIFM010000378 DBFIFM010000000
VERSION     DBFIFM010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.380"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            105..257
                     /locus_tag="N7C74_004856"
     CDS             105..257
                     /locus_tag="N7C74_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU2434541.1"
                     /translation="MDMLNLGNNESLVCGVFPNHDGTFTAMTYTRSKTFKTEAGAHRW
                     LARNAN"
     gene            738..926
                     /gene="dicB"
                     /locus_tag="N7C74_004857"
     CDS             738..926
                     /gene="dicB"
                     /locus_tag="N7C74_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU2434542.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNKAFTEDAINMRKYEPYLLNDN
                     SILSRIALIKLGIFGERQ"
     gene            923..1111
                     /locus_tag="N7C74_004858"
     CDS             923..1111
                     /locus_tag="N7C74_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGU2434543.1"
                     /translation="MTSAFALVMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            1207..>1778
                     /locus_tag="N7C74_004859"
     CDS             1207..>1778
                     /locus_tag="N7C74_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGU2434544.1"
                     /translation="MSKIFICAAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPGIPRPALDSWDTEYMQENRWDEESASFVPVEPESDPM
                     NVNFDKLSPEVQNAVLVKFDTCENITVDMVISAQELLQEDMATFGGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCEPGAKWPEIQA"
BASE COUNT          503 a          401 c          431 g          443 t
ORIGIN      
        1 gtagccagcc cgaggcgtat gaacatgacg gcgggaacac tttgtataac agcgcagcag
       61 gtttttagtt ccgcgacccg gcgttaaggg taaatgaggt caacatggat atgctcaatc
      121 ttggcaacaa tgaatctctg gtgtgcggag tattccccaa ccacgacggc acgtttaccg
      181 cgatgacgta taccagaagt aaaacgttta aaaccgaagc tggcgcgcat cgctggttag
      241 caagaaacgc taactgatta gcgccagtaa aaacaggttt ccacaggtta atttaccctg
      301 aaaagtcagg gcataacacg aaagcgcacg gcgaagttag tctctctgta taggtcgtcg
      361 ttaaatttaa ttcgaccgtg cgcttctggt tgtggcaatc cgcgaaatgg cgcggcggta
      421 agtatggcgg ggttattcct tcccccgttg aggacaccgg gttgtcaggt tgaccatacg
      481 cttaagtgac accccagcca caacaacctc tgttatcact tttctggtga ttcggcggaa
      541 atggatatcc gcccttttta aagtgaattt tgtgatgcgg tgaatgcggc tatgcgcacg
      601 cggaacagtt aaagcagtaa ggcggtcttt tactggcgta acgagcatca actaacccga
      661 cgttaattgt taactggtta acgtcacctg gaggcaccag gcactgcatc acaaaattca
      721 ttgttgagga cgcgataatg gaaacgttat taccaaacgt taatacgtct gaaggttgtt
      781 ttgatattgg tgttctgctc agtaataaag cgtttactga ggatgccatt aatatgcgga
      841 aatatgagcc ttatctgctc aatgataatt ccattctttc ccgaattgcc cttattaaac
      901 ttggtatttt cggagaacgt caatgacttc agcatttgca ctggtgatga cggtttttct
      961 tataacgggt gaatcacaga atgttattac cggaatttat gccagtaaag aatcctgcct
     1021 ccaggcaaga gacgagcaaa aaatttctgg tgaatgccta ccggtaaaaa aagtatcgct
     1081 gtacctgaat aacgaaacac cggctggata acccgccagc catattaacg ccataccaac
     1141 ggattaaaaa tgccagcaat ggcagggatt cgttcaccct gaaatctgta atgaggttaa
     1201 aacacaatga gtaaaatctt tatttgcgct gctattcctg acgaacaggc cataaaagaa
     1261 gatagcgctg ttgcggtggc cactgccatt gaagccggtg atgagcgtcg cgcacgcgca
     1321 aaatttcact ggcaatttct ggagcaattc ccggcagctc aggactgcgc ttataaattt
     1381 attgtctgcg aggataaacc cggcataccc cgccctgccc tcgattcctg ggataccgaa
     1441 tatatgcagg aaaaccgctg ggatgaggag tcagcttcct ttgtaccggt cgaaccagaa
     1501 tccgatccga tgaacgtcaa ttttgacaag ctgtcccctg aagtacagaa cgcagtcctg
     1561 gttaaattcg acacatgcga aaacatcacc gttgatatgg tgattagcgc acaggaatta
     1621 ctacaggaag acatggcaac attcggcgga catatcgttg aagcgttgat gaaaatgcca
     1681 gaagttaacg ccatgtatcc tgaacttaaa ctgcatgcca tcgggtgggt taagcataaa
     1741 tgtgagcctg gcgctaaatg gcctgaaatt caggcaga
//
LOCUS       DBFIFM010000379         1772 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.381, whole genome shotgun sequence.
ACCESSION   DBFIFM010000379 DBFIFM010000000
VERSION     DBFIFM010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.381"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..156
                     /locus_tag="N7C74_004860"
     CDS             <1..156
                     /locus_tag="N7C74_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU2434545.1"
                     /translation="QWPGKAAIGRLKGDELTQYNLWLDYLDALELVDTSGAPDIEWPT
                     PPAVQAR"
     gene            complement(128..721)
                     /locus_tag="N7C74_004861"
     CDS             complement(128..721)
                     /locus_tag="N7C74_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU2434546.1"
                     /translation="MKPVFDENGLATVPGDMRCFYYDAETSEYTGWSDEYINTGVSMP
                     ACSTGIDPGENIPGRVAVFTGKGWSHEEDHRNETVYSIENGAAVTVDYIGAIKDGYVT
                     ISPLTPYDKWDGEKWVTDTEAQHSAAVDAAEAQRQSLIDAAMASISLIQLKLQAGRKL
                     TQAETTRLNAVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(721..1515)
                     /locus_tag="N7C74_004862"
     CDS             complement(721..1515)
                     /locus_tag="N7C74_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2434547.1"
                     /translation="MAKNDFKAFATDRNANVMSQEEWEALPALISGFTAGKASSAQVN
                     KVIRQASFIAAALAQFVSDKTQRDVLDNGDLPGFVELLGSGFAVEYLSRKNPFGDIKS
                     DGTVKTALQNLGLGEGAPAIGVPFFWPSAAMPNTVIDSWSCMVFLKFNGAKFSATDYP
                     VLAKVFPSLVLPEARGDFIRIWDDGRGADGGRELLSWQAATNFSQFAGNIGEGAGHAI
                     NFHDGIAGNQPGFSRFNFTSNSVGDGVNFVAVRPRNIAFNFLVRAK"
     gene            complement(1515..>1772)
                     /locus_tag="N7C74_004863"
     CDS             complement(1515..>1772)
                     /locus_tag="N7C74_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replication protein"
                     /protein_id="HGU2434548.1"
                     /translation="IANIDNQDMTISVWVFPDTDISDVSRELIAAIKQGYLTVKAAGV
                     WAGGIETPSVETPSEGSKFFGFDMDNEFISGFDVGAWGVLL"
BASE COUNT          461 a          483 c          375 g          453 t
ORIGIN      
        1 caatggcccg gtaaagcggc tattggtcgt ctgaaaggtg acgaactgac gcaatataat
       61 ttgtggctgg attatctgga cgcactggag ctggtcgata cttccggtgc gccagatatt
      121 gaatggccta cgcctccggc agttcaggcc agatgacatc cggcgcggtg ctggtatctg
      181 ttgccgtcac cgcgtcaatg taatccagca cggcgttaag tcgggttgtt tctgcctgag
      241 tcagtttccg tccggcctgt aacttcagct gaatcagact aatggaagcc attgctgcat
      301 caatcagtga ttggcgctgt gcttctgccg cgtctactgc ggcactgtgt tgtgcctcag
      361 tgtctgtcac ccatttctca ccatcccatt tatcgtatgg cgttaacggt gaaatcgtga
      421 cataaccgtc tttgatggca ccgatataat ccactgtaac agctgcgcca ttttcgattg
      481 agtaaacagt ctcattgcga tggtcttctt catggctcca tcccttacct gtaaatactg
      541 ccactcttcc cggaatgttt tcgccagggt caataccagt ggaacaggcg ggcatactta
      601 cgccagtatt aatatattca tcagaccagc ccgtatactc agatgtttca gcatcataat
      661 aaaaacaacg catatcgccc ggcactgtag ccagcccatt ttcatcaaaa acaggtttca
      721 ttatttagcc ctcaccagaa agttaaatgc aatatttcgc ggtctgactg caacaaaatt
      781 cacaccatca cccacagagt tactggtgaa attaaatcgt gaaaatcctg gctgatttcc
      841 ggcgatgcca tcatgaaagt taattgcgtg tcccgcacct tcgcctatat tcccggcaaa
      901 ctgagaaaag tttgtagctg cctgccagct taataattcg cgaccaccgt ctgcacctcg
      961 cccgtcatcc cagatacgaa tgaaatcacc gcgggcttca ggtaatacca gcgaaggaaa
     1021 cactttcgcc agcacaggat aatcagtggc agagaatttc gcgccgttga acttcaaaaa
     1081 caccatacag gaccagctgt cgattacagt atttggcatt gcagcggacg gccagaagaa
     1141 cggaacgcca atagctggag caccttctcc caaaccaagg ttttgaagag ccgttttcac
     1201 cgtgccgtcc gatttgatat cgccaaacgg attcttgcgg ctcaggtatt caacagcaaa
     1261 ccccgatccc agcaattcaa caaaaccggg cagatcacca ttatcaagca catcccgttg
     1321 cgttttatca cttacaaact gggccagagc tgcagcaata aagctggcct gccgaataac
     1381 cttattgact tgcgcactgg atgctttccc tgctgtaaat ccggatataa gcgcaggcaa
     1441 cgcttcccat tcctcctgcg acataacatt ggcatttcga tcagttgcaa acgctttaaa
     1501 gtcatttttc gccatcagag taatactccc catgccccta catcaaaacc actgatgaat
     1561 tcgttatcca tatcaaaacc aaaaaattta gagccttccg atggagtttc caccgaaggt
     1621 gtttcaatgc cccccgccca taccccggcg gcttttactg ttagataccc ctgtttaatt
     1681 gccgcaatta actcacgcga tacatctgaa atatcagtat caggaaagac ccagaccgat
     1741 atcgtcatgt cctggttatc gatgttggcg at
//
LOCUS       DBFIFM010000380         1762 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.382, whole genome shotgun sequence.
ACCESSION   DBFIFM010000380 DBFIFM010000000
VERSION     DBFIFM010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.382"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(76..1488)
                     /gene="pykF"
                     /locus_tag="N7C74_004864"
     CDS             complement(76..1488)
                     /gene="pykF"
                     /locus_tag="N7C74_004864"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HGU2434549.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1496..1690)
                     /locus_tag="N7C74_004865"
     CDS             complement(1496..1690)
                     /locus_tag="N7C74_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434550.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT          431 a          422 c          436 g          473 t
ORIGIN      
        1 cttctggtta tcgattaaat aaaaaaagcg cccatcaggg cgcttcgata tacaaattaa
       61 ttcacaaaag caatattaca ggacgtgaac agatgcggtg ttagtagtgc cgctcggtac
      121 cagtgcacca gaaaccataa ctacaacgtc acctttgtgt gccagaccgc tctgcagagc
      181 cagttcttta cccagacggt agaaatcatc agtagaagtg atctctttaa ccagctgcgg
      241 cacaacgcct ttgctcagta ccaactgatg agccgttttt tcgttggtgg tcagtgccag
      301 gatggtggca tccgggaagt atttacgtac tgcgcgagca gatttaccgc cctgggtagc
      361 aaccacaatc agcggagcat ccagtttttc agcagtttca acggcaccac ggcataccgc
      421 ttcggtaatg cgcagtttac ggttgtcatt gttgaactcg agacggctgt tcatcacgcg
      481 gtcggtacgt tcgcagatgg tcgccatgat agaaaccgct tccagcgggt atttaccttt
      541 tgcggattca ccagacagca tcactgcgtc agtaccgtcg aggatggcgt ttgcaacgtc
      601 accggcttct gcgcgagtcg ggcgtgggtt tttgatcatg gaatccagca tctgggtcgc
      661 agtgataacg actttacgtg cacggataca tttttcgatc atcatcttct gggcgaagat
      721 aacttcttct accgggattt ctacacccag gtcgccacgc gcaaccatga tgccgtcaga
      781 ggcttcgagg atttcgtcga agttgttgag gccttcctgg ttttcgattt tggagatgat
      841 gtggatgttt tcgccgccgt gcgctttcag gtgctcacgg atttcgataa cgtcagaacg
      901 cttacgaata aaggaagcag cgacaaagtc tacgccttgt tcgcaaccaa agatcaggtc
      961 ctgtttgtct ttttcagcca gtgctggcag ggcaatggaa acgccaggca ggttcacacc
     1021 tttgttttca cccaggtcgc cgttgttcag cactttacag ataactttgt taccttcaat
     1081 ggcagtaact tccataccga tcagaccatc gtcaaccagt acggtgttgc caacagacag
     1141 gtcagttgtg aaaccttcat acgttaccgc aaccatttcg ctgttgccga taacagattt
     1201 atcagtagtg aaagtaaagg tctgaccagc tttcagagaa acgtcgttac cgccttccag
     1261 tttcatggtg cggatttccg gacctttggt atcaagcagg atagcggcgg ttttaccagt
     1321 tttgctcatc acgttgcgca gattctgaat gcgctgaccg tgttctgcat agtcaccatg
     1381 agagaagttc agacgcataa cgttcatgcc agcgtccagc attttagcta acatctcttc
     1441 agattcggtt ttcggtccga tggtgcaaac aattttggtc tttttcatga cagtcttagt
     1501 ctttaagttg agaaggatgg gagaaacttg ctttctgggc gcactgccgg aaagcgaatc
     1561 cggtattacg aaagtggtgg tgcctcgctc taaggatagg tgacatcgaa aaagcgtgca
     1621 gaggaatgtg tgcttgtgtc tcagcccaac gtgagtgcct gtttttgagt cgttgagttc
     1681 tacagtccaa agtgctgaaa ccattcaaga gtcaattggc gcgcattata cgctgaaatt
     1741 aatccaaaag gaaaacaaaa ac
//
LOCUS       DBFIFM010000381         1761 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.383, whole genome shotgun sequence.
ACCESSION   DBFIFM010000381 DBFIFM010000000
VERSION     DBFIFM010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.383"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(72..269)
                     /locus_tag="N7C74_004866"
     CDS             complement(72..269)
                     /locus_tag="N7C74_004866"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HGU2434551.1"
                     /translation="MIGKAIIKAQYNKQVPLTLVAMSVLTAMSIACQNQVDVCSPGNL
                     RGAVNIYTMVLEKGKRPGERY"
     gene            complement(496..792)
                     /locus_tag="N7C74_004867"
     CDS             complement(496..792)
                     /locus_tag="N7C74_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2434552.1"
                     /translation="MPDQSGHCRTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            complement(971..1707)
                     /locus_tag="N7C74_004868"
                     /pseudo
     CDS             complement(971..1707)
                     /locus_tag="N7C74_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          482 a          373 c          366 g          540 t
ORIGIN      
        1 tgaagactgt attattcacc acgaaatatc attcatattt ctcattctta atacctccgg
       61 aaattatttc actaatacct ttcccccggt cttttccctt tctccagaac catagtgtaa
      121 atattaacgg ccccacgtaa atttccggga ctgcatacgt caacctgatt ctgacaggca
      181 attgacatcg ctgttaatac tgacattgca accagagtca gaggtacttg tttattgtat
      241 tgagctttaa ttattgcctt ccctatcatt gatggtcatg catatactga atacgacata
      301 tccggtttga tgtaaggcat aaatgcttcc tttttcgtaa tacgtttcgg ttatagcagc
      361 aattaccgct acaatagcga caatagctgc tctttcaaca atgaatgctg gaaattctgt
      421 ttatcaatta aacttctggc taaattgttc ttatgcttct ttttcctcct gaggtgatca
      481 cgtgatgaca aaaacttatc tgctcatggt catatctgaa gaaagcgtat gtctcacttc
      541 gtcattaaca cctttgactg ctgatctggc aggtacagta tgtaaagtaa gccattcgtc
      601 aatagctgat gaacgccagc ccacagaacc actaccaaga cgtaccggcc tggggaaggt
      661 tgcatcgtag tatttcgata acggattcat tttttcgtaa attgtcgaac gagatatacc
      721 taacaattgg ctcagttcag gcatccgtaa gatgcgtgaa ggagtcctgc agtgtccgga
      781 ttgatctggc attttatccc ccatagtgtg gtgaactgtc gttacagttt gtattccccg
      841 ggtaaatttg tctgcgtaaa aaaatgggtt gagaaaaaag acaataaata attaaaaatc
      901 aatatgatag aaaacgacag cattagtgtt attttacttt tcccttatcg ctgtcgattc
      961 tcattatcat tcatggcaaa aaaacagcaa aagaggccct ggtaatgact cctgtcagta
     1021 gtacatgtgt tttcgataaa tgaaccaatg gcttttcgtg aatttccagg gagcgtaaaa
     1081 agcaaatcgt tttccgggca ggtcgtttga tgtgagtacg aagtgtcaga ttgttgcgct
     1141 caatgcgccg ggtaaatatc ttgccgacaa gatgcttatc ctgtggcatt tctctggtat
     1201 aactgctccg gttgtctctg gttatcatgc ctgcggagaa tggcttcagg aattccggca
     1261 actcacggca tgtttcatca gtgcgaggac caaaagtgta agccagcaca ccgtcagctt
     1321 tggtcttata cgcgtaccag tgccactgct gacgagcttt gttttcgaca aaactccatt
     1381 gttcatcaag ttcacagata agtgccacat cgacactggc ggctggagat atggttactt
     1441 tacgtggtga gcgtttttta aagtgagaat gacggtgttg atatccacct tcagcgttct
     1501 ggagctatcg cgaaccccgg ctccgttatg aaccatttca acaatttgct ctttaatgcc
     1561 tggttttcgg gtttcgtaga tgtaattcag ttgaaataca cgtttgcagg attagcactg
     1621 gaaacgctca tgatctgagg tgctgtgatc ataacggtac actttatagg aattacagcg
     1681 gggagaatat actgtaactg ttgccatatg gtctccagat accaatagaa tacaacatta
     1741 atctatcgtc agaaggcatc a
//
LOCUS       DBFIFM010000382         1755 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.384, whole genome shotgun sequence.
ACCESSION   DBFIFM010000382 DBFIFM010000000
VERSION     DBFIFM010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.384"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..175
                     /locus_tag="N7C74_004869"
     CDS             <1..175
                     /locus_tag="N7C74_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434553.1"
                     /translation="GALERLFMQTGVSWSDPLRAQYFSVQFSEGIRRAYRHTLATSPY
                     SSYRNPVRRQRFF"
     gene            175..825
                     /locus_tag="N7C74_004870"
     CDS             175..825
                     /locus_tag="N7C74_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HGU2434554.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLQELCDYYNDAVRAVILARP
                     DAGASLETISCVPGARQVLPDGVIQLLDVICLSDGSAVRPLSREVLDAQYPEWPTMKG
                     IPECFISNDLSPRVFWLFPAPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVEWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGGV"
     gene            822..>1755
                     /locus_tag="N7C74_004871"
     CDS             822..>1755
                     /locus_tag="N7C74_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HGU2434555.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTVVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTATKNTQATQSKESAAASAKSASDSAKTATSRAA
                     EAGQKATDATEAATRAVTAAGNAEESSTRAGESEKAAGADAEKARQHAEKARLAQESA
                     GEILKRAEAATVSAEEARRMAENARGPRGPQGETGPKGDVGPKGETGPVGPQGPAGPK
                     GERGDVGAQGAVGPAGPRGEKGEQGERGPQGIPGL"
BASE COUNT          418 a          395 c          575 g          367 t
ORIGIN      
        1 gggggcactt gagcggttgt tcatgcagac tggtgtttca tggtcagatc cgttacgcgc
       61 acagtatttt tctgtgcagt tttctgaggg gatccgtcgg gcatatcgtc atacactggc
      121 gacaagcccg tactcttcat accgcaaccc tgtacgcagg cagaggtttt tctgatgacg
      181 acgattacag aaatcatcgg acgtgtgaat acacaactgg ttgacccgat gatggttcgc
      241 tggcccctgc aggaattgtg cgattattac aatgatgctg tgagggcagt gattctggcg
      301 agaccggatg ctggcgcaag cctggaaaca ataagttgtg ttcctggcgc ccgtcaggtt
      361 ttgcccgatg gtgtaataca acttcttgac gtgatatgcc tcagtgacgg tagtgcagtc
      421 agaccattat cccgggaggt gctggatgcg cagtatcctg agtggcccac aatgaagggt
      481 attcctgaat gttttatcag caacgacctg tccccgcgcg tattctggct gtttcctgct
      541 cctgacaaag agataagtat tgatgcagtg gtaagccgga taccggaggc agtgtatgtt
      601 ctgacgcagg acgatgatac gccagttcca ctggaagagg cttatgttaa cccactggtg
      661 gagtggatgt tgtttcgcgc tttcagtaag gatgctgccg gtggcgcaga atcggggctg
      721 gctgcgcagc attatcagag ttttgttgag caacttggga tcaaacaggg ggcagacagt
      781 gcattgtctg cccgtaaaaa agtgtttaac ggaggtggag tgtgagtgtt gttgtttcgg
      841 ggacgctgaa atctcctgat ggtgaggcga tatcaggagc aaatattacc ctgacggcgc
      901 tgacagtttc accggatgcg ctcagcggca ccagtgcgtc ggcagtgacc cgtgaaggtg
      961 gatattacgg aatgacgatg gatccggggg agtatgcggt ttcggtgacg gtgaaaggga
     1021 agactgttgt ctacggacgt gtgcgtattg aggggaccga aagtacggtg acgctcaata
     1081 tgctgttacg ccgcagtctt gttgaggtta gcatacccgg agaactgctg acagatttcc
     1141 ggcagataca gaataatgtg gctgatgacc ttgccactat tcgtcgcctg aatgaagaca
     1201 cggcgacaaa aaacactcag gccacacagt caaaagaaag tgcagcagcc agtgcgaaga
     1261 gtgcatctga cagtgcaaag acggcaacca gcagggcggc tgaagccgga caaaaagcga
     1321 ctgatgccac tgaggctgcg acccgtgcag tcacagcagc ggggaatgca gaggaaagct
     1381 cgacccgtgc cggagagtct gaaaaagccg ccggagctga tgcagaaaaa gccagacagc
     1441 atgctgaaaa ggccaggctg gcgcaggaga gcgccggaga gatccttaag cgggcagagg
     1501 ctgccactgt cagtgctgaa gaggccagac gtatggctga gaatgcacgg gggccccggg
     1561 ggcctcaggg agaaactggt ccgaaggggg atgtcggtcc taaaggcgaa acaggtccag
     1621 tgggccctca agggcccgca gggccgaaag gtgagcgtgg tgacgttggt gctcaggggg
     1681 ctgtagggcc tgctggtccg cgtggtgaga agggcgaaca gggggagcga ggaccgcagg
     1741 gaataccagg cctga
//
LOCUS       DBFIFM010000383         1743 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.385, whole genome shotgun sequence.
ACCESSION   DBFIFM010000383 DBFIFM010000000
VERSION     DBFIFM010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.385"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            48..1724
                     /gene="tir"
                     /locus_tag="N7C74_004872"
     CDS             48..1724
                     /gene="tir"
                     /locus_tag="N7C74_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HGU2434556.1"
                     /translation="MPIGNLGHNPNVNNSIPPAPPLPSQTDGAGGRGQLINSTGPLGS
                     RALFTPVRNSMADSGDNRASDVPGLPVNPMRLAASEITLNDGFEVLHDHGPLDTLNRQ
                     IGSSVFRVETQEDGKHIAVGQRNGVETSVVLSDQEYARLQSIDPEGKDKFVFTGGRGG
                     AGHAMVTVASDITEARQRILELLEPKGTGESKGAGESKGVGELRESNSGAENTTETQT
                     STSTSSLRSDPKLWLALGTVATGLIGLAATGIVQALALTPEPDSPTTTDPDAAASATE
                     TATRDQLTKEAFQNPDNQKVNIDELGNAIPSGVLKDDVVANIEEQAKAAGEEAKQQAI
                     ENNAQAQKKYDEQQAKRQEELKVSSGAGYGLSGALILGGGIGVAVTAALHRKNQPVEQ
                     TTTTTTTTTTTSARTVENKPANNTPAQGNVDTPGSEDTMESRRSSMASTSSTFFDTSS
                     IGTVQNPYADVKTSLHDSQVPTSNSNTSVQNMGNTDSVVYSTIQHPPRDTTDNGARLL
                     GNPSAGIQSTYARLALSGGLRHDMGGLTGGSNSAVNTSNNPPAPGSHRFV"
BASE COUNT          491 a          378 c          455 g          419 t
ORIGIN      
        1 aaataaagga ttgttatcta tttacataaa ataaaaagga gatatttatg cctattggta
       61 atcttggtca taatcccaat gtgaataatt caattcctcc tgcacctcca ttaccttcac
      121 aaaccgacgg tgcagggggg cgtggtcagc tcattaactc tacggggccg ttgggatctc
      181 gtgcgctatt tacgcctgta aggaattcta tggctgattc tggcgacaat cgtgccagtg
      241 atgttcctgg acttcctgta aatccgatgc gcctggcggc gtctgagata acactgaatg
      301 atggatttga agttcttcat gatcatggtc cgctcgatac tcttaacagg cagattggct
      361 cttcggtatt tcgagttgaa actcaggaag atggtaaaca tattgctgtc ggtcagagga
      421 atggtgttga gacctctgtt gttttaagtg atcaagagta cgctcgcttg cagtccattg
      481 atcctgaagg taaagacaaa tttgtattta ctggaggccg tggtggtgct gggcatgcta
      541 tggtcaccgt tgcttcagat atcacggaag cccgccaaag gatactggag ctgttagagc
      601 ccaaagggac cggggagtcc aaaggtgctg gggagtcaaa aggcgttggg gagttgaggg
      661 agtcaaatag cggtgcggaa aacaccacag aaactcagac ctcaacctca acttccagcc
      721 ttcgttcaga tcctaaactt tggttggcgt tggggactgt tgctacaggt ctgatagggt
      781 tggcggcgac gggtattgta caggcgcttg cattgacgcc ggagccggat agcccaacca
      841 cgaccgaccc tgatgcagct gcaagtgcaa ctgaaactgc gacaagagat cagttaacga
      901 aagaagcgtt ccagaaccca gataatcaaa aagttaatat cgatgagctc ggaaatgcga
      961 ttccgtcagg ggtattgaaa gatgatgttg ttgcgaatat agaagagcag gctaaagcag
     1021 caggcgaaga ggccaaacag caagccattg aaaataatgc tcaggcgcaa aaaaaatatg
     1081 atgaacaaca agctaaacgc caggaggagc tgaaagtttc atcgggggct ggctacggtc
     1141 ttagtggcgc attgattctt ggtgggggaa ttggtgttgc cgtcaccgct gcgcttcatc
     1201 gaaaaaatca gccggtagaa caaacaacaa caacaactac tacaactaca actacaagcg
     1261 cacgtacggt agagaataag cctgcaaata atacacctgc acagggcaat gtagataccc
     1321 ctgggtcaga agataccatg gagagcagac gtagctcgat ggctagcacc tcgtcgactt
     1381 tctttgacac ttccagcata gggaccgtgc agaatccgta tgctgatgtt aaaacatcgc
     1441 tgcatgattc gcaggtgccg acttctaatt ctaatacgtc tgttcagaat atggggaata
     1501 cagattctgt tgtatatagc accattcaac atcctccccg ggatactact gataacggcg
     1561 cacggttatt aggaaatcca agtgcgggga ttcaaagcac ttatgcgcgt ctggcgctaa
     1621 gtggtggatt acgccatgac atgggaggat taacgggggg gagtaatagc gctgtgaata
     1681 cttcgaataa cccaccagcg ccgggatccc atcgtttcgt ctaaatatat ccataatcat
     1741 ttt
//
LOCUS       DBFIFM010000384         1723 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.386, whole genome shotgun sequence.
ACCESSION   DBFIFM010000384 DBFIFM010000000
VERSION     DBFIFM010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.386"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            19..633
                     /gene="evgA"
                     /locus_tag="N7C74_004873"
     CDS             19..633
                     /gene="evgA"
                     /locus_tag="N7C74_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HGU2434557.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            638..>1723
                     /locus_tag="N7C74_004874"
     CDS             638..>1723
                     /locus_tag="N7C74_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HGU2434558.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGG"
BASE COUNT          541 a          365 c          328 g          489 t
ORIGIN      
        1 ttgcaaaggg aataatctat gaacgcaata attattgatg accatcctct tgctatcgca
       61 gcaattcgta atttattgat caaaaacgat attgaaatct tagcagagtt gactgaaggc
      121 ggaagtgccg ttcagcgggt ggaaacactt aagcctgata tcgtcatcat tgatgtcgat
      181 atccccggag ttaacggtat ccaggtgtta gaaacgctga ggaagcgcca atatagcgga
      241 attattatta tcgtctccgc taaaaatgac catttttacg ggaaacattg tgctgatgct
      301 ggcgctaatg gtttcgtgag taaaaaagaa ggcatgaaca atatcattgc ggctattgaa
      361 gctgcaaaaa atggctactg ctatttcccc ttctctctca accggtttgt tggaagttta
      421 acgtccgacc agcaaaaact cgactcctta tcgaaacaag aaattagtgt catgcggtat
      481 attcttgatg gcaaggataa taatgacatt gctgaaaaaa tgttcatcag caacaaaact
      541 gtcagcactt ataaaagtcg cctgatggaa aaattagaat gtaaatcact gatggatctt
      601 tacacattcg cacaacgtaa caaaatcggc taaccacatg aagtttttac cctatatttt
      661 tcttctctgt tgtggtcttt ggtcgaccat aagtttcgca gacgaagatt acatcgaata
      721 tcgtggcatc agtagtaaca accgtgtcac acttgatcca ctacgtctga gcaacaagga
      781 attacgttgg ttagcgagca aaaaaaatct tgtgattgca gtacacaagt cccaaacggc
      841 tacgttgttg cataccgatt cgcagcaacg ggttcgtggt attaatgctg attatttaaa
      901 tcttttaaaa agagcgttaa atatcaaatt aacactccgg gaatacgcag atcatcaaaa
      961 agcaatggac gcgctggaag aaggtgaagt cgatatagtg ttatcacatt tagttgcttc
     1021 gccgcctctt aatgatgaca ttgctgcaac caaaccactg ataattacct ttccggcgct
     1081 ggtcaccacc cttcacgatt caatgcgacc gcttacctca tcaaaaccag taaatattgc
     1141 tcgagtagca aattatcccc ctgacgaggt aattcatcaa tcatttccaa aagcaacaat
     1201 tatctctttt acaaatttat atcaggcatt agcatccgtc tcagccggac agaatgatta
     1261 ctttattggt agtaacatca ttaccagcag tatgatttcc cgctatttca ctcactcctt
     1321 aaatgtagtg aaatattata actcaccgcg tcaatataac ttcttcttaa ccagaaaaga
     1381 atctgtcatt cttaatgaag tactcaatcg atttgttgat gctttaacaa atgaagttcg
     1441 ctatgaagta tcacaaaatt ggcttgatac aggaaacctg gcctttctga acaagccatt
     1501 agaactcact gaacatgaaa aacagtggat taagcagcat cccgatttaa aggtgctgga
     1561 aaatccttac tcgcccccct attctatgac ggatgaaaat ggctcggttc ggggcgttat
     1621 gggggacatt cttaatatta ttaccttgca aacaggttta aatttttctc cgatcaccgt
     1681 ttcacacaat atccatgctg gaacacagct gaatcccggc gga
//
LOCUS       DBFIFM010000385         1714 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.387, whole genome shotgun sequence.
ACCESSION   DBFIFM010000385 DBFIFM010000000
VERSION     DBFIFM010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.387"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(109..552)
                     /locus_tag="N7C74_004875"
     CDS             complement(109..552)
                     /locus_tag="N7C74_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HGU2434559.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLDLAYVGE"
     gene            709..1638
                     /gene="metA"
                     /locus_tag="N7C74_004876"
     CDS             709..1638
                     /gene="metA"
                     /locus_tag="N7C74_004876"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HGU2434560.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          418 a          470 c          387 g          439 t
ORIGIN      
        1 aagcgaaagg ccatcctgac ggatggcctt tttgcgttgg tgcaaaaaaa tgccggatgc
       61 gacgctggcg cgttttatcc agcttacgca ggcacgatag ggggcagctt attcccccac
      121 atacgccaga tccagcaacg gatacggttt ccccaaatcg tccacctcag agcgtcccgt
      181 aaccttaaaa cccaccttct tatagaaccc aaccgcctgc tcattttgct cattaacatt
      241 ggttgtcagt tccggggcca ttgagagcgc atgcttcacc agcatccggc ccacgccgca
      301 gccgcgcaca tcaggatcga taaacagcgc atccatatgc tgcccactta acaacataaa
      361 cccaaccggc tgatcccgtt cattaaccgc gacccacaac ggtgcttccg gcagaaagga
      421 acgaaccagc tcttccagct cggcccgata ctctgctgat aaaaaatcgt gggtggcatc
      481 gacagatcga caccaaatcg caacgagttc ctccccttcc tcatgccgtg agcggcgaat
      541 actaataacc attttctctc cttttagtca ttcttatatt ctaacgtagt cttttccttg
      601 aaactttctc accttcaaca tgcaggctcg acattggcaa attttctggt tatcttcagc
      661 tatctggatg tctaaacgta taagcgtatg tagtgaggta atcaggttat gccgattcgt
      721 gtgccggacg agctacccgc cgtcaatttc ttgcgtgaag aaaacgtctt tgtgatgaca
      781 acttctcgtg cgtctggtca ggaaattcgt ccacttaagg ttctgatcct taacctgatg
      841 ccgaagaaga ttgaaactga aaatcagttt ctgcgcctgc tttcaaactc acctttgcag
      901 gtcgatattc agctgttgcg catcgattcc cgtgaatcgc gtaacacgcc cgcagagcat
      961 ctgaacaact tctactgtaa ctttgaagat attcaggaac agaactttga cggtctgatt
     1021 gtaaccggtg cgccgctggg cctggtggag tttaatgatg tcgcttactg gccgcagatc
     1081 aaacaggtgc tggagtggtc gaaagatcac gtcacctcga cgctgtttgt ctgctgggcg
     1141 gtacaggcag cgctcaatat cctctacggc attcctaagc aaactcgcac cgacaaactc
     1201 tctggcgttt acgagcatca tattctccat cctcatgcgc ttctgacgcg tggctttgat
     1261 gattcattct tggcaccgca ttcgcgctat gctgactttc cggcagcgtt gattcgtgat
     1321 tacaccgatc tggaaattct ggcagagacg gaagaagggg atgcatatct gtttgccagt
     1381 aaagataagc gcattgcctt tgtgacgggc catcccgaat atgatgcgca aacgctggcg
     1441 caggaatatt tccgcgatgt ggaagccgga ctagacccgg aagtaccgta taactatttc
     1501 ccgcacaatg atccgcaaaa caaaccgcga gcgagctggc gtagtcacgg taatttgctg
     1561 tttaccaact ggctcaacta ttacgtctac cagatcacgc catacgatct acggcacatg
     1621 aatccaacgc tggattaatc ttctgtgata gtcgatcgtt aagcgattca gcaccttacc
     1681 tcaggcacct tcgggtgcct tttttatttc cgaa
//
LOCUS       DBFIFM010000386         1690 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.388, whole genome shotgun sequence.
ACCESSION   DBFIFM010000386 DBFIFM010000000
VERSION     DBFIFM010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.388"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..>1690
                     /locus_tag="N7C74_004877"
     CDS             <1..>1690
                     /locus_tag="N7C74_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGU2434561.1"
                     /translation="GAVTVAIRREYKQTWKTLDNELACALWPGDVDAGNIDGTIHRWA
                     TNEVIDKDREDWKRISASMRKQPEALGYDRQTIFGLVRERPIDIHKDPVALNKYISEY
                     LTTKGVFEHEETDQSSTDALQPSAAQTAPVETAESDTQKNEILVEAEPSVEREGPFYF
                     VFTDKGGEKYGRANKLSGLDKALAAGGTEISKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     SEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQSRDAE
                     DQNQPNVKQNEPEAEQNSPDTQQNGPEEQQPEPAVQQELEKVCTACGQTGGGNCPDCG
                     AVMGNATYLETFDEENQAEAQKNDPEEMEGTEHLHKENTGSDQYHASDNKTGETANPL
                     IKVNGHHEISSTSRLWHHLMIDLETMGKNPDAPINSIAGKFFDPATGEMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSSILTDEITLDDALLQFREFIDENSGEFFVQVWG
                     NGATFDNVILRRSYERQGIPCPWRYTNDRDVRTMVALGLVMDFDARTTIPFEGER"
BASE COUNT          522 a          396 c          438 g          334 t
ORIGIN      
        1 ccggggctgt cactgttgcc attcgccgcg aatacaaaca gacatggaaa acactcgaca
       61 atgaactggc ctgcgccctc tggcccggtg atgtggatgc aggaaacatt gacggtacca
      121 tccatcgctg ggcgacaaat gaggttatcg acaaggatcg cgaagactgg aagcgtatct
      181 cagcatcaat gcgcaaacag cccgaagcac ttggctatga ccgtcagact atttttggcc
      241 ttgttcgcga acgtccgatc gatattcaca aagatcccgt tgcactgaac aaatatatca
      301 gtgaatacct gacgacaaag ggcgtgtttg aacatgaaga aacagaccag agctctactg
      361 atgctctcca gccgtcagca gcacaaactg ctccagtgga gacggcagaa tccgatactc
      421 aaaaaaatga aatcctggtg gaagctgaac catctgtaga gcgtgaagga ccattttatt
      481 tcgtctttac cgataagggc ggggaaaaat acggcagggc aaacaaactt tctggtctgg
      541 acaaggcgct ggctgccggc ggtaccgaaa tctcaaaaga agaatatttt gcccgaaaaa
      601 atggcacata cacgggctta ccgcaaaatg tggataccgc tgaagattcc gaacaatcag
      661 agccggtaaa agttaccgct gacgaagtaa acaaaattat gcaggcagcc aatatcagcc
      721 agcctgacgc cgataaattg cttgctgcat cacgtggtga atttgttgaa gggattagcg
      781 acccgaatga tccgaaatgg gtgaaaggga tccagagtcg cgacgctgag gaccagaatc
      841 agcccaacgt gaaacaaaat gagccagaag cggaacaaaa cagcccggat acgcaacaaa
      901 acgggccaga agaacaacaa ccagaaccag cagtgcaaca ggaactggaa aaagtttgca
      961 ccgcatgcgg tcagaccggt ggcggcaact gtcctgactg tggtgcggtg atggggaacg
     1021 caacctacct ggaaacattc gatgaagaga atcaggctga agctcagaaa aatgatccgg
     1081 aggaaatgga aggcactgaa catctgcaca aggagaacac tggcagcgat cagtatcacg
     1141 ccagcgataa taaaactggc gaaacagcaa atcccttaat taaagtgaac ggtcatcatg
     1201 aaatctcatc caccagcagg ttgtggcacc atctgatgat tgaccttgaa acaatgggaa
     1261 aaaatcctga tgcgccaata aactctatag ccggtaagtt ttttgatccg gcaaccggag
     1321 agatggggcc agaattcagc aaaactatcg atctggaaac cgcaggtggg gtcatcgatc
     1381 gggacaccat taagtggtgg ctgaaacagt cacgcgaagc acaatcctcc attctgaccg
     1441 atgaaatcac gttggatgat gcactgctgc aattccggga atttatcgac gaaaactccg
     1501 gtgaattttt tgttcaggtc tggggtaacg gtgcaacttt cgacaacgtg attttacgcc
     1561 gttcatatga acggcagggg atcccctgcc cgtggcgtta caccaatgat cgcgatgtaa
     1621 gaacgatggt tgctctggga ctggtgatgg atttcgacgc aagaacgact attccattcg
     1681 aaggtgaacg
//
LOCUS       DBFIFM010000387         1690 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.389, whole genome shotgun sequence.
ACCESSION   DBFIFM010000387 DBFIFM010000000
VERSION     DBFIFM010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.389"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(117..1400)
                     /gene="gltA"
                     /locus_tag="N7C74_004878"
     CDS             complement(117..1400)
                     /gene="gltA"
                     /locus_tag="N7C74_004878"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HGU2434562.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDVKH"
     gene            complement(1606..1689)
                     /locus_tag="N7C74_004879"
     CDS             complement(1606..1689)
                     /locus_tag="N7C74_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434563.1"
                     /translation="MSPKINLCNSVEVLTNSFGTVISGRQV"
BASE COUNT          450 a          391 c          418 g          431 t
ORIGIN      
        1 gtaattatgt atttgtgtac gattacgttt ttttgtcgat agacataact aacgaacaaa
       61 aaaaagcccg ccagatattc aggcgggcta aacgatatat ggttcaaatc agataattaa
      121 tgtttaacat ctgatttaaa gtctcgtttt tcatagccgg tgtacaactg gcgcggacgg
      181 gcgatcttca tgccgtcact gtgcatttcg ctccagtgag cgatccagcc aacggtacgg
      241 gccatagcga agatgacggt gaacatggaa gacggaatac ccatcgcttt caggatgata
      301 ccagagtaga aatcgacgtt cgggtacagt ttcttctcga taaagtacgg gtcgttcagc
      361 gcgatgtttt ccagctccat agccacttcc agcaggtcat ctttggtgcc cagctctttc
      421 agaacttcat ggcaggtttc acgcattacg gtggcgcgcg ggtcgtaatt tttgtacacg
      481 cggtgaccga agcccatcag gcggaaagaa tcatttttat ctttcgcacg acgaacaaat
      541 tccggaatgt gtttaacgga gctaatttct tccagcattt tcagcgccgc ttcgttagca
      601 ccaccgtgtg caggccccca cagtgaagca atacctgctg cgatacaggc aaacgggttc
      661 gcacccgaag agccagcggt acgcacggtg gaggtagagg cgttctgttc atggtcagcg
      721 tgcaggatca gaatacggtc catagcacgt tccagaatcg gattaacttc atacggttcg
      781 cacggcgtgg agaacatcat attcaggaag ttaccggcat aggagagatc gttgcgcgga
      841 taaacaaatg gctgaccaat ggaatacttg taacacatcg ctgccatggt cggcattttc
      901 gacagcaggc ggaacgcggc aatttcacga tgacgaggat tgttaacatc cagcgagtcg
      961 tgatagaacg ccgccagcgc gccggtaata ccacacatga ctgccattgg atgtgagtcg
     1021 cgacggaaag cgtggaacag acgggtaatc tgctcgtgga tcatggtatg acgggtcacc
     1081 gtagttttaa attcgtcata ctgttcctga gtcggttttt caccattcag caggatgtaa
     1141 caaacttcca ggtagttaga atcggtcgcc agctgatcga tcgggaaacc gcggtgcagc
     1201 aaaatacctt catcaccatc aataaaagta attttagatt cgcaggatgc ggttgaagtg
     1261 aagccagggt caaaggtgaa cacacctttt gaaccgagag tacggatatc aataacatct
     1321 tgacccagcg tgcctttcag cacatccagt tcaacagctg tgtccccgtt gagggtgagt
     1381 tttgcttttg tatcagccat ttaaggtctc cttagcgcct tattgcgtaa gactgccgga
     1441 acttaaattt gccttcgcac atcaacctgg ctttacccgt ttgttatttg gctcgccgct
     1501 ctgtgaaaga ggggaaaacc tgggtacaga gctctgggcg cttgcaggta aaggatccat
     1561 tgatgacgaa taaatggcga atcaagtact tagcaatccg aattattaaa cttgtctacc
     1621 actaataact gtcccgaatg aattggtcaa tacttccaca ctgttacata agttaatctt
     1681 aggtgaaata
//
LOCUS       DBFIFM010000388         1687 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.390, whole genome shotgun sequence.
ACCESSION   DBFIFM010000388 DBFIFM010000000
VERSION     DBFIFM010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.390"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..426
                     /locus_tag="N7C74_004880"
     CDS             <1..426
                     /locus_tag="N7C74_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HGU2434564.1"
                     /translation="NDARTLFEFTSGVNVIESPIIYRDESMRTACSPDGLCSDGNGLE
                     LKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMWVTRKDAWYFANYDPRMKREGLHYV
                     VVERDEKYMASFDEMVPEFIEKMDEALAEIGFVFGEQWR"
     gene            423..605
                     /locus_tag="N7C74_004881"
     CDS             423..605
                     /locus_tag="N7C74_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HGU2434565.1"
                     /translation="MKHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLPLS"
     gene            578..769
                     /locus_tag="N7C74_004882"
     CDS             578..769
                     /locus_tag="N7C74_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HGU2434566.1"
                     /translation="MHKASPVELRTSIGMAHSLAQIGVRFVPIPVETDEEFHTLAASL
                     SQKLEMMAAKAEANERDPA"
     gene            846..1061
                     /locus_tag="N7C74_004883"
     CDS             846..1061
                     /locus_tag="N7C74_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGU2434567.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            1160..1381
                     /locus_tag="N7C74_004884"
     CDS             1160..1381
                     /locus_tag="N7C74_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HGU2434568.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            1378..>1687
                     /locus_tag="N7C74_004885"
     CDS             1378..>1687
                     /locus_tag="N7C74_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU2434569.1"
                     /translation="MSEINYQALREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLIESDAQAYANAEFIAEANPATVLELLDERERNQQYIKRRDQEN
                     E"
BASE COUNT          493 a          367 c          440 g          387 t
ORIGIN      
        1 aacgacgcca gaaccctgtt tgagttcact tccggcgtga atgttattga atccccgatc
       61 atctatcgcg acgaaagtat gcgtaccgcc tgctctcccg atggtttatg cagtgacggc
      121 aatggccttg agctgaaatg cccgtttacc tcccgggatt tcatgaagtt ccggctcggt
      181 ggtttcgagg ccataaagtc ggcttacatg gcccaggtgc agtacagcat gtgggtgaca
      241 cgaaaagatg cctggtactt tgccaactat gacccacgta tgaagcgtga aggactgcat
      301 tatgtcgtgg ttgagcggga tgaaaagtac atggcgagtt ttgacgagat ggtgccggag
      361 ttcatcgaaa aaatggacga ggcactggct gaaattggtt ttgtatttgg ggagcaatgg
      421 cgatgaagca tcctcacgat aatatccggg taggtgcgat cactttcgtc tactccgtta
      481 caaagcgagg ctgggtattt cccggccttt ctgttatcag aaatcccctg aaagcacagc
      541 ggctggctga ggagataaat aataaacggg gagctgtatg cacaaagcat ctcccgttga
      601 gttaagaacg agtatcggga tggcacatag cctcgctcaa attggagtca ggtttgtgcc
      661 aataccagta gaaacagacg aagaatttca tacgttagcc gcatcccttt cacaaaagct
      721 ggaaatgatg gcggcgaaag cagaagcaaa cgagagagac ccggcatgac aacaacagaa
      781 tgcatttttc tggcagcagg cttcatattc tgtgtgctta tgcttgccga catgggactt
      841 gttcaatgac acctcagcag gaaaacgccc ttcgcagcat tgcccgtcag gctaattctg
      901 aaatcaaaaa agccagacag cagtttccgg ataaaaacgt cgatgacatt tgccgtagcg
      961 tactgaagaa gcaccgcgaa acggtaacgc taatgggatt cacaccgact catttaagtc
     1021 tggcgatcgg catgttaaac ggcgtcttta aggagcgatg aacatgaaaa gcaaaatcat
     1081 cagggagcta caggctcctt ttttattgtt cgcattcacc ctcaagcgta ttaaccaaca
     1141 attcagggat taatgaaaga tggcagatat cattgattca gcatcagaaa ttgaagaatt
     1201 acagcgcaat acagcaataa aaatgcgtcg cctgaactac cagactgtat ccgcaactca
     1261 ttgttgtgag tgtggcgatc cgatagatga acgaagacgc ctggcagttc agggttgtcg
     1321 gacttgtgca agttgccagg aggatctgga gcttatcagt aaacagagag gttcgaagtg
     1381 agcgaaatta attatcaggc actgcgtgaa aaggcagaaa aagcaactaa aggaagctac
     1441 atcgtagggc atacatctgt taaccagcac ggcaatttaa caggagtttt tgtttgccaa
     1501 aaatggaaag gagaacccgg tggcgtgatt gcggaatgtc atgttaactg cctgattgaa
     1561 tcagatgctc aggcttatgc aaacgctgaa ttcatagcag aggctaaccc ggctaccgtg
     1621 ctggaactgc tggatgaacg ggaaagaaac cagcaataca tcaaacgccg cgaccaggag
     1681 aacgagg
//
LOCUS       DBFIFM010000389         1686 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.391, whole genome shotgun sequence.
ACCESSION   DBFIFM010000389 DBFIFM010000000
VERSION     DBFIFM010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.391"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..234
                     /locus_tag="N7C74_004886"
     CDS             <1..234
                     /locus_tag="N7C74_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU2434570.1"
                     /translation="GKLIDTGFCIFALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLD
                     HLKTLIAKGANQCARAGDKLPDLLDEYIRATTE"
     gene            206..1686
                     /locus_tag="N7C74_004887"
                     /pseudo
     CDS             206..1686
                     /locus_tag="N7C74_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
BASE COUNT          407 a          395 c          478 g          406 t
ORIGIN      
        1 ggaaaactga ttgacaccgg gttctgtatt tttgccctca gtaagctggc aatggcgtta
       61 tccagtacgc ttgattccat ccctttatcc atgcagcgac agtttcctga tttaacaccg
      121 cgccatcttg accatctgaa aacccttatt gctaaggggg caaatcagtg tgcgcgggca
      181 ggggataaat taccggattt acttgatgaa tatatcagag caacaactga ataatatgat
      241 gagcgctgtc acaactgcat tacagcccct gataagggca ttgccggtga cgccagttga
      301 atgggctgat caaaattatt atctgcctaa agaatcttca tatggtgagg gcgaatggaa
      361 aacgctgcca ttccagatcg ccatcatgaa cagtatgggg aatgatcaga tccgcactgt
      421 taatctgatt aaatctgccc gtgttggcta tacaaagatg ttgctggggg gggggcgggt
      481 attttattga gcataaatcc cgaaacagtc tgctttttca gcccacggat tctgccgctg
      541 aagattttat gaagtctcac gtggaggcga cgattcggaa cgtgccatgc ctgaaagacc
      601 tttccccatg gctgggtcgt aaacatcgtg acaatactct cacgctgaaa cgcttttcat
      661 cgggcgtcgg tttctggtgc ctgggcggcg ctgccgccaa aaactaccgt gaaaaatccg
      721 ttgacgtggt ctgctatgac gaactttcct cgttcgagcc ggatgtcgaa aaagagggct
      781 cgccaaccct gctgggggat aagcgtattg aggggtcggt gtggccaaaa tccattcgcg
      841 gctcgacgcc taaaatcaaa ggcacctgcc agatcgaaaa agccgctaac gagtcggcgc
      901 attttatgcg tttttatgtg ccctgcccgc actgtgggga ggcgcagtat ctgaaatttg
      961 gcgatgagtc cacgcctttt gggcttaaat gggagaagga cagccctgaa agtgttttct
     1021 acctctgtga acatcatggc tgcgtgatcc atcagtctga actggaccag agcaacgggc
     1081 ggtggatctg tgaaaacacg ggcatgtgga cccgtgacgg tctgacgttt ttcagcgccc
     1141 ggggtgatga aattccgccg ccgcgctcca tcatgttcca tatctggacg gcgtacagtc
     1201 cgttcaccac ctgggtacag attgtctatg actggctgga tgcactgaaa gatcccaacg
     1261 gcctgaaaac ctttgtgaac accacgctgg gcgagacctg ggaagaggcc gtgggcgaaa
     1321 aactcgatca ccaggtactg atggataagg tggtgcgtta cacggcggcg gtgcctgccc
     1381 gggtggttta tctgacggcg ggcattgact cgcagcgaaa ccgttttgag atgtatgtct
     1441 ggggatgggc tccgggagag gaagcctttc tggtggataa aatcatcatt atggggcgtc
     1501 ctgatgagga agagacgctg ttacgtgtgg atgcggcgat caacaaaaaa taccgccatg
     1561 cggatggcac cgaaatgact atttcccgtg tctgctggga caccgggggg atcgatggtg
     1621 aaattgttta tcagagatca aaaaaacacg gtgttttccg ggtgctgccg gtaaaaggcg
     1681 catctg
//
LOCUS       DBFIFM010000390         1678 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.392, whole genome shotgun sequence.
ACCESSION   DBFIFM010000390 DBFIFM010000000
VERSION     DBFIFM010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.392"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..504)
                     /locus_tag="N7C74_004888"
     CDS             complement(<1..504)
                     /locus_tag="N7C74_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434571.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEPEPKSMRA
                     LPSGVVPAVHQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAQQNLAAWMIDVIRHAMNGIQERE
                     LSLAELSW"
     gene            complement(506..784)
                     /locus_tag="N7C74_004889"
     CDS             complement(506..784)
                     /locus_tag="N7C74_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434572.1"
                     /translation="MAHDTKLYNSDDSAVFASRRGRCFHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(952..1107)
                     /gene="hokD"
                     /locus_tag="N7C74_004890"
     CDS             complement(952..1107)
                     /gene="hokD"
                     /locus_tag="N7C74_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGU2434573.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVF
                     TAYEPEE"
BASE COUNT          422 a          409 c          426 g          421 t
ORIGIN      
        1 ccaggataat tcagccagcg ataattcccg ttcctgtatg ccattcattg cgtggcggat
       61 gacgtcaatc atccaggcgg ccagattctg ttgagcaagt tgctcaagtg attcggatgt
      121 ctggtcgcgc agctggttgt cgcagtgcca gcacaacacc atcgcgccgg taccgtaacg
      181 atgtatgaca gtttcgctgt gatgataatc accatgaggc cactggcagg atttcacgtg
      241 acgtaataac cagtcagaca gtgcaccagc gccacccgca gcacggatca cccgctcatc
      301 gctgaaaaat ggcagtagtg atttatcttc cgccagcggc tggtgaacgg caggtacaac
      361 cccggacggc agagctcgca tgctttttgg ttccggctcc accagtattc tgccgttatg
      421 gaatgctgac attgattcac ggcctggctt aacgataacc agaccgagtt ccggtaccag
      481 aacaggtcga agtaataccc gcacattacc tccagatccg ttgctggaat gtgcgggacg
      541 gacgcggtgg ccgttcggag taagggagcc tgacggagat tatccagtga cggtagtcga
      601 ggctaagggc ttttttaacc tcgcatccgc gcctgcggta acactgaatg agccattcgg
      661 cctgttcttc agtgcatggg ggatgctggt accagtcaga tttgaatgcg tgaaaacacc
      721 gtccgcgcct gctggcaaag acggcagaat catcagaatt gtataatttg gtatcgtgcg
      781 ccatcggttg tctctgctgg cgcagcaggt gccagttgtt caggctggcg tgcgaattgt
      841 aaaccagaat gccaggaaaa aacaaaaccc gccgaagcgg gttaagtgcg ggtgcgttga
      901 ggatgcctga ttcatcagag gtggcgaggg atttctcccc cgccaggtct cttactcctc
      961 aggttcgtaa gctgtgaaga cagcgacctc cgtctggccg gttcggactc gtacctcgca
     1021 gaggtctttc ctcgttacca gtgccgtcac tatgacggtt aaacagatga cgatcagggc
     1081 gattaacatc gccttttgct gcttcatagc ctgcttctcc ttgcctttcg gcacgtaaga
     1141 ggctaaccta gatttgccgt tcatagattg agcctcagat taatgttagg cgtcttgcag
     1201 gacgcgtaat gttaactggg gcttttctct atctgccgtt ggtgttcatg cccgaggcag
     1261 atagcctcaa gcacccgcag ctattctact taacttccca ttacctcgcc aatatgaaat
     1321 cggtcagaaa gacagacgtc ccataaggac aacaacggaa aaagagattt tgcttgatgc
     1381 tatttacctc tgcaccatga agttgcgcca aaatcccagt ggtgataaca ggaatttggt
     1441 tgttggtgat tggggaaaga agtacgttag actgtgggtt agttcattga catgatagtt
     1501 acctgccttc aatttcctaa gcaagcgatg taaacgataa tcaatatcaa atgtattgac
     1561 aggtaaaata ataacgatgt acctttgctg ataacatata tcagtcttaa atcataatgg
     1621 aggtagataa aataactaaa cacacacaac aaatttttta tcagtgacat agcatatc
//
LOCUS       DBFIFM010000391         1651 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.393, whole genome shotgun sequence.
ACCESSION   DBFIFM010000391 DBFIFM010000000
VERSION     DBFIFM010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.393"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..>1651
                     /gene="rhsC"
                     /locus_tag="N7C74_004891"
     CDS             <1..>1651
                     /gene="rhsC"
                     /locus_tag="N7C74_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HGU2434574.1"
                     /translation="RQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVTSGHPVNP
                     LLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADIRLQLRDN
                     TLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQALPEELR
                     LSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGRTQTFHRE
                     AAGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEY
                     GRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAVVYDRSGKQVRSFTYDDK
                     YRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRREVL
                     HTQGEGGLKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRIT
                     TPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGRLIQETAPDGDITRYRYDNPHS
                     DLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRF"
BASE COUNT          335 a          500 c          534 g          282 t
ORIGIN      
        1 gcgtcagggc gacatgacgc agtatggcgg tagcattgtt cagggttcag ccggggtgcg
       61 cattggtgcc cccaccggcg tggcctgttc ggtgtgcccc ggcggagtga cgtccggcca
      121 tccggtcaat cccctgctcg gtgcaaaggt ccttcccggt gaaaccgaca tcgccctgcc
      181 cggcccgctg ccgttcatcc tctcccgcac ctacagcagt taccggacaa aaacgcccgc
      241 gccggtgggg agcctcggcc ccggctggaa aatgcctgcg gatatccgct tacagctgcg
      301 cgataacaca ctgatactca gtgataacgg cggcagaagc ctgtattttg agcacctgtt
      361 tcccggtgag gacggttaca gccgcagcga gtcactctgg ctggtgcgcg gcggcgtggc
      421 gaaactggat gaaggtcacc ggctggccgc actctggcag gcgctgccgg aagaactccg
      481 cttaagtccg catcgttatc tggcgacaaa cagtccgcag gggccgtggt ggctgctcgg
      541 ctggtgtgag cgggtgccgg aagcggatga ggtgctgcct gcgccgctgc cgccgtaccg
      601 ggtactgacc gggctggtgg accgcttcgg gcgcacacag acgttccacc gcgaagccgc
      661 cggtgaattc agcggcgaaa tcaccggcgt gacggatggt gccgggcgtc acttccggct
      721 ggtactgacc acgcaggcgc agcgggcaga agaagcccgg cagcaggcca tttccggcgg
      781 gacggaaccg tccgcttttc ctgataccct gccgggttac accgaatatg gccgggacaa
      841 cggcatccgt ctgtctgccg tgtggctgac gcacgacccg gaatacccgg agaatttacc
      901 tgccgcgccg ctggtgcgct atggctggac gccgcgcggc gaactggcgg tggtgtatga
      961 ccgtagtggc aaacaggtgc gcagctttac ttacgatgat aaataccggg gccggatggt
     1021 ggcgcaccgt cacacgggcc ggccggaaat ccgttaccgt tacgacagcg acgggcgggt
     1081 gacagaacag ctaaacccgg caggcttaag ctacacgtat cagtatgaga aagaccgcat
     1141 caccatcacc gacagcctga accgccgtga agtcctgcac acgcagggtg aaggcgggct
     1201 gaagcgggtg gtgaaaaagg aacacgcgga cggcagcgtc acgcagagtc agtttgacgc
     1261 ggtgggcagg ctcagggcac agacggatgc cgcaggcagg acaacagaat acagcccgga
     1321 tgtggtgacg ggcctcatca cgcgcatcac cacgccggat ggcagggcat cggcgtttta
     1381 ctataaccac cacagccagt taacgtcagc caccgggcct gacgggctgg aaatacgccg
     1441 ggaatatgat gaatggggcc gtctgattca ggaaactgcc cctgacggcg atatcacccg
     1501 ctaccgttat gataatccac acagtgactt accctgcgca acggaagatg ccaccggcag
     1561 ccggaaaacc atgacgtgga gccgttacgg tcagttgctg agcttcaccg actgttccgg
     1621 ttatgtaacc cgttatgacc atgaccgctt c
//
LOCUS       DBFIFM010000392         1651 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.394, whole genome shotgun sequence.
ACCESSION   DBFIFM010000392 DBFIFM010000000
VERSION     DBFIFM010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.394"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..518)
                     /locus_tag="N7C74_004892"
     CDS             complement(<1..518)
                     /locus_tag="N7C74_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2434575.1"
                     /translation="MPLNDMQIRRAKPEAKAYTFGDGLGLSLLIEPNGSKSWRFRYRY
                     AGKPKMISLGVYPTITLADARSRRDEARKLVAEGKNPSEVRKEQKLAMQTESENAFEK
                     IAREWHQLKSAKWSAGYASDIMEAFKNDIFPYVGTRPVGEIKPLELLNVLRKIEKRGA
                     LEKMRKVRQRCS"
     gene            1005..1439
                     /locus_tag="N7C74_004893"
                     /pseudo
     CDS             1005..1439
                     /locus_tag="N7C74_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(1481..>1651)
                     /locus_tag="N7C74_004894"
     CDS             complement(1481..>1651)
                     /locus_tag="N7C74_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGU2434576.1"
                     /translation="EYLLSTRCYPISKVAKVCGYASVSYFTYVFRRYFGVSPSQYSQR
                     SSESKILTHQGI"
BASE COUNT          477 a          388 c          367 g          419 t
ORIGIN      
        1 tcggagcaac gctgccgcac tttgcgcatt ttctccaacg caccacgttt ctcaatttta
       61 cgcagaacgt tcagcagctc tagcggttta atctctccca caggccttgt tccgacataa
      121 ggaaaaatgt cgttcttaaa cgcttccatg atgtctgatg catatcccgc cgaccattta
      181 gcagatttaa gttgatgcca ctctctggct atcttttcga aggcgttctc tgactctgtt
      241 tgcatagcta gcttttgctc ttttcgaacc tcactagggt tctttccttc tgccacaagt
      301 tttcgagctt catcacgacg ggaacgagca tcggcaaggg tgatcgttgg gtaaacacca
      361 agcgagatca ttttgggttt gccggcatag cgatagcgga accgccaact cttgcttcca
      421 ttaggttcta taagtaatga caaccctagc ccatctccaa atgtataggc tttagcttca
      481 ggcttagcgc ggcgaatctg catatcgttt aaaggcatgt gtataggaat ccagaccgaa
      541 caggaacata tacacaatcc tatacacatt cagtaccgga ttctactgga tggttacgga
      601 ctactatgga cttaaaagaa taaaaaatgt tataaatcag agattttatg gactaatacg
      661 gacgtttgag gaagttgaga tggtgccgat aataggagtc gaacctacga ccttcgcatt
      721 acgaattata agaatccgct tctaattcaa agcattaccc catcaacact gcgctcacac
      781 gtcccaccac atcaaaacat gtaaagcctt gcaagccatt gcgaggcctt atgtgtctca
      841 gttttgtccc accttgtatt acgacttgca tagccaatga agataaacgt gacgacaaac
      901 ggcgcagcag tcttcttttc tttcacgact tctccagcat gcataccttt ctgccatgac
      961 tgaagtgaat gcctgttatg agcaaggatt ggcccagatg aatcgccctg gactgttgcg
     1021 atcgtgaagc catagactgg gccgcaagca cgggaggtta cgataaagcg acagtgcagg
     1081 atgtcatgtt aggagcgata gaaaaacgct ttggcgataa agtaccggaa cagtccatcc
     1141 agtggctgac agataacggt tcagcgtata gagcgcatga aacacggcag ttcgccagag
     1201 agttgaatct ggagccctgc acaacggcaa tcagcagtcc tcagagtaat ggaattgcag
     1261 aaaggttcgt gaaaacgatg aaggaagact acatcgcgtt catgccgaaa ccgaatgtaa
     1321 gaacggcatt gcataatctt gcagtggcga tcgaacatta caatgaaaac catccgcaca
     1381 gtgcgttggg ttatcgctct ccgcgagaat atcgacgtca gcgggtaacg ttaacttaag
     1441 ataccccccc tgtctggaaa taagggggca agaacaatga tcagattccc tggtgagtaa
     1501 gaattttact ttctgaactc ctctgagagt attgacttgg agaaacacca aaataacgtc
     1561 taaatacata agtaaagtat gagacactgg cataaccaca gaccttagct actttactaa
     1621 taggataaca acgagtgctg agtaaatatt c
//
LOCUS       DBFIFM010000393         1639 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.395, whole genome shotgun sequence.
ACCESSION   DBFIFM010000393 DBFIFM010000000
VERSION     DBFIFM010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.395"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(1..153)
                     /locus_tag="N7C74_004895"
                     /pseudo
     CDS             complement(1..153)
                     /locus_tag="N7C74_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247981.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
     gene            293..529
                     /locus_tag="N7C74_004896"
     CDS             293..529
                     /locus_tag="N7C74_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU2434577.1"
                     /translation="MSRLITLRDWAKEEFGDLAPSERVLKKYAQGKMMAPPAIKVGRY
                     WMIDRNSRFVGTLAEPQLPINANPKLQRIIADGC"
     gene            519..>1639
                     /locus_tag="N7C74_004897"
     CDS             519..>1639
                     /locus_tag="N7C74_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HGU2434578.1"
                     /translation="MAASPRSHKISIPNLYCKLDKRTGKVYWQYKHPLSGRFHSLGTD
                     ENEAKQVATEANTIIAEQRTRQILSVNERLERMKGRRSDITVTEWLDKYNSIQEDRLQ
                     HNELRPNSYRQKGKPIRLFREHCGMQHLKDITALDIAEIIDAVKAEGHNRMAQVVRMV
                     LIDVFKEAQHAGHVPPGFNPAQATKQPRNRVNRQRLSLPEWQAIFDSVSRRQPYLKCG
                     MLLALVTGQRLSDICNLKFSDIWDDMLHITQEKTGSKLAIPLNLKCDALNITLREVIS
                     QCRDAVVSKYLVHYRHTTSQANRGDQVSANTLTTAFKKAREKCGIKWEQGTAPTFHEQ
                     RSLSERLYREQGLDTQKLLGHKSRKMTDRYNDDRGKDWI"
BASE COUNT          535 a          362 c          351 g          391 t
ORIGIN      
        1 aatcccacgc tttaccagaa cactttttgc agcagaacac ttgtgcggca atatcccggt
       61 taattcggaa agttgctcgt tgctaaccca atccagtttt ttattaaagc catatgtttt
      121 gcgcatgaca gccagaaaga ccagaagctg gtgaaaaatt gataaattca atactgcatt
      181 tctcagcatt aaatttatct ctatgaccag tcaagagatg tacctgccat gagcttaata
      241 tcatgtcaga tatatcggtc acaaactccc tcagcagcta agaggaggac aaatgtctcg
      301 actaatcact ttacgggact gggctaaaga agaatttggg gacttagcac caagtgagcg
      361 agttctgaaa aaatacgcgc aagggaaaat gatggcccca cccgctataa aagttggtcg
      421 ctactggatg attgaccgaa attcccgttt tgtaggaacg cttgcagaac cgcaactccc
      481 aataaacgca aacccaaaac tccaacggat aatcgctgat ggctgctagc ccccgatctc
      541 acaaaatctc tatacccaat ttatattgca aattagataa gcgaaccgga aaggtatatt
      601 ggcaatacaa acatccacta tccggtcgtt ttcatagctt aggaactgat gagaatgaag
      661 caaaacaagt tgctactgaa gcaaatacca ttattgctga acaacgtacc cgacaaatat
      721 taagcgtcaa tgagcgtctg gaaagaatga aaggcaggcg ctcagacatt acggtgacag
      781 aatggcttga taaatataat tctatccagg aggacaggct gcaacataat gaactaagac
      841 ccaactccta tcggcaaaaa ggcaaaccca tccgtctttt ccgtgagcat tgtggaatgc
      901 aacacctcaa ggatattacc gcacttgata ttgccgaaat aattgatgct gtaaaggctg
      961 aaggtcataa caggatggcg caagtcgtga gaatggtgtt gatcgacgtc ttcaaagaag
     1021 cacaacacgc aggacatgtt ccgccaggat ttaacccagc gcaggcaaca aaacaaccgc
     1081 gaaatcgagt aaaccgtcaa agattatcac tgcccgaatg gcaggcaata tttgacagcg
     1141 taagcagacg gcagccctat ttaaaatgcg ggatgctact tgctcttgtc actggacaac
     1201 gtttaagcga tatctgcaat ttgaaattct ctgatatctg ggacgacatg ttgcacatta
     1261 ctcaggaaaa aaccggttca aaacttgcta ttccgcttaa cctgaaatgc gatgctctga
     1321 atattaccct tcgtgaagtt atatctcagt gcagggatgc tgttgttagt aaatatctgg
     1381 tccattaccg tcacactacc tctcaagcaa acagaggaga ccaggtgtct gcaaatactc
     1441 ttacaacggc ttttaaaaag gccagggaaa aatgtggcat aaaatgggag caaggaactg
     1501 cgcccacatt tcatgagcag cgatctctgt cagaacggtt atatcgggaa cagggtctgg
     1561 atacgcaaaa gttgttaggt cataaatcca gaaaaatgac cgaccgatac aatgatgatc
     1621 gtggtaaaga ctggattat
//
LOCUS       DBFIFM010000394         1629 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.396, whole genome shotgun sequence.
ACCESSION   DBFIFM010000394 DBFIFM010000000
VERSION     DBFIFM010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.396"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..1148)
                     /locus_tag="N7C74_004898"
     CDS             complement(<1..1148)
                     /locus_tag="N7C74_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HGU2434579.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELHSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFASSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAWVGGANEASG
                     VTKTLSGMLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVA
                     LAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQER"
     gene            complement(1196..1405)
                     /locus_tag="N7C74_004899"
     CDS             complement(1196..1405)
                     /locus_tag="N7C74_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HGU2434580.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSLAAQS"
     gene            complement(1501..>1629)
                     /locus_tag="N7C74_004900"
     CDS             complement(1501..>1629)
                     /locus_tag="N7C74_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HGU2434581.1"
                     /translation="DLQRVFTPDDREQVLAVYGPVHARLLRQALELIADAESARKK"
BASE COUNT          328 a          558 c          399 g          344 t
ORIGIN      
        1 cgttcctgtg tggccgccag acgggcctct gcagcaatct gcatctccgt cccgcgggct
       61 gccgccacgg cctgctgagc acggtacacg gctgcccttg cccgcgccgt ggcaatctgc
      121 gttcccctga actgtgcttc cgccagtgca acttcattac gtgcagccgt cacaagtcct
      181 gccgtggcag acatcgctcc ggaggccata ttgccaaagt accgggcaac cccgacggca
      241 accagcgcgc ccacggctgt tgccacatta tcaatctgtc cggcaacacc gttcagcatg
      301 ccggagagcg tttttgtcac cccgctggcc tcattcgcac cgcccaccca ggccataaag
      361 gcgttttcca cctttgtgat actactggaa accgtttccg gcatggccgc atattcatca
      421 cgtaatatcc ccagctggct gattaacgcg gggaccactt tatccgctgt cagttttccg
      481 tcatccgcca ttgccttcag atctttacgg gccacgccca tacccgcagc cagtgcacgt
      541 acgatccggt caccactttc attgaccgaa ttaaactcct caccacgcaa tacaccctgc
      601 gccagcgcct gactgaactg ggtgatcacc gagcccgcct ctgccgtact ggcaccggag
      661 attttcagcc ctgtcgaaat ggcctccgtc accttcagca catcatcagc actgtaacca
      721 tattcacgca tcgaggcagc cgaacgggca aacagggccg cattatccga aaatgcggtg
      781 cctgtccgct ggctgatatc catcagcact ttctgtgatg acgcaaattc atcggatgac
      841 tgtgatgcct gtttcagacg ggcattcacg gagctccatt catccgccag cgaaatcagg
      901 tgtccggtgg caaaggcacc ggcaaacgca ccggtcattc cgacagccga agcgcggatt
      961 tccgtcaact ggctgtgcag ctcagccaga gcccggcgct gctccctggc tgccgcagca
     1021 gcctgacgtc cgccattctg cagggtccgg taatattcac tgcccatacg ggacgcccgc
     1081 tggatctccg actggaatga ctgtgaattt gccgaaattt tgataatcag ttcacgtaac
     1141 gtcgccattc acctttctcc gggcgtaaaa aaaccgcctc agcggttctc atcattcatg
     1201 actgtgctgc aaggctcagc gcgtcttcca gcgccgcaaa cggatccacc tccggcttat
     1261 cctcatcctc gccccagcag agcatggcgt ccttcagtgc aacattcatc ccctgtgccc
     1321 cgaaaaccgc tttcacgatc tgtgcattac ggatatcccc gcgctcatca cccagcgggg
     1381 atatcctgtc gaactccatc cacatcatcg cctcgctcgc actcaggctg tggcgcagtt
     1441 cggataaggt gcgccccaga cggagcgcaa gtcgcatcag aaagcgaatt tccgggcggg
     1501 ctactttttt ctggccgact ctgcatcagc gatcagttcc agtgcctgac gcagcaaccg
     1561 ggcatgtacc ggaccataga cggccagcac ctgctcacgg tcgtccggag tgaacacccg
     1621 ctgcaggtc
//
LOCUS       DBFIFM010000395         1622 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.397, whole genome shotgun sequence.
ACCESSION   DBFIFM010000395 DBFIFM010000000
VERSION     DBFIFM010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.397"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..>1622)
                     /locus_tag="N7C74_004901"
     CDS             complement(<1..>1622)
                     /locus_tag="N7C74_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU2434582.1"
                     /translation="LSIDAASRTLTLDREVTLPETGAATVNLINGSGKPVSVAITAHP
                     APDRIQVSTLPDGVETYGVWGLSLPSLRRRLFRCVSVRENTDGTFAITAVQHVPEKEA
                     IVDNGARFEPQSGTLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPKVVKGVSFMLRL
                     TVAADDGSERLVSTARTTETTYRFTQLAPGNYRLTVRAVNAWGQQGDPASVSFRIAAP
                     AAPSQIELTPGYFQITAVPRLAVYDPTVQFEFWFSETRITDIRQVETTARYLGTGLYW
                     IAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGQPSDDASGYLDFFKGEIGKTHLAQE
                     LWTQIDNGQLAPDLAEIRTSITDVSNEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLN
                     SMWAVKLQQMQDGRLYIAGIGAGIENTSDGMQSQVLLAADRIAMINPANGNTKPMFVG
                     QGDQIFMNEVFLKYLTAPTITSGGNPPAFSLTSDGKLTAKNADISGSVNANSGTLNNV
                     TINENCRVLGKLSANQIEGDLVKTVGKAFPRD"
BASE COUNT          319 a          491 c          432 g          380 t
ORIGIN      
        1 gtcccggggg aaagctttgc ccactgtttt aacgagatcg ccttcaatct ggttcgcgga
       61 cagttttccc agaacccgac agttctcgtt aatcgtgacg ttgttgagcg tcccggagtt
      121 cgcattcaca ctgccactga tatccgcatt tttagcggtc agctttccgt ctgatgtcag
      181 ggaaaatgcc ggaggattgc cgccactggt aatggtggga gccgtcagat atttcaggaa
      241 cacttcattc atgaatatct gatcgccctg accaacaaac atcggctttg tgttgccatt
      301 cgcaggatta atcatcgcaa tcctgtctgc cgccagcagc acctgactct gcatgccgtc
      361 agaggtgttc tcaataccgg caccgatacc cgcaatataa aggcgtccgt cctgcatctg
      421 ctgcagcttc actgcccaca tgctgttcag gttattattt gtatcaacct gaaccttctg
      481 tatctgctgg atcgctgcac tctggtcttc cagtttctta ttgacggtct gcgtgatttc
      541 attactgaca tccgtgatgg acgttctgat ttccgccagg tcaggcgcaa gctgaccgtt
      601 atcaatctga gtccacaact cctgagccag atgggttttc cctatctctc ctttgaaaaa
      661 atccagatag ccggatgcat catcactcgg ctggccaaca gcctccacaa atgccgattt
      721 gccaacggtg ttcacactgc ggatataaaa gtaataatca tggcccggtt tgatattgat
      781 actggcggct atccagtaca gccccgtgcc aaggtagcgg gctgtggttt caacctgcct
      841 gatatcggta atccgcgttt ccgaaaacca gaactcaaac tgtaccgtcg ggtcatacac
      901 cgcaagacgc gggaccgctg ttatctgaaa atagcccggt gtcagctcaa tctgtgacgg
      961 cgctgccggt gcggcaatcc ggaacgatac cgacgccgga tcgccctgct gcccccacgc
     1021 atttaccgcc cggactgtca gcctgtagtt ccccggcgcc agttgcgtga agcggtatgt
     1081 ggtttccgtc gtccgggccg tgctgaccag ccgctcactg ccgtcatccg cggccacggt
     1141 caggcgaagc ataaagctca cgcccttcac caccttcggc gtgtcccatt tcgcctgcgc
     1201 cagatactga ccgtcagctg cgctcacctc caccgtcagg tgctgcactg ccggagggat
     1261 gacgctgttc agggtgcctg actgcggctc aaagcgggca ccgttatcca cgatggcttc
     1321 tttttccggt acgtgctgca ccgccgtgat ggcaaaggtg ccgtccgtgt tttcccggac
     1381 ggagacacag cggaacaggc gacggcgcag tgacggcagg gagagccccc acacaccgta
     1441 tgtctccacg ccatcaggca gggtgctgac ctgtatccgg tccggcgcgg ggtgtgcagt
     1501 gatggccacg ctcaccggct taccgctgcc gttaatcagg ttcaccgtgg cggcaccggt
     1561 ctccggcagg gtcacctcac ggtccagtgt cagggtgcgg ctggcggcat cgatggacag
     1621 ga
//
LOCUS       DBFIFM010000396         1586 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.398, whole genome shotgun sequence.
ACCESSION   DBFIFM010000396 DBFIFM010000000
VERSION     DBFIFM010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.398"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(1..138)
                     /locus_tag="N7C74_004902"
                     /pseudo
     CDS             complement(1..138)
                     /locus_tag="N7C74_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            362..487
                     /locus_tag="N7C74_004903"
                     /pseudo
     CDS             362..487
                     /locus_tag="N7C74_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203849.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            complement(547..1053)
                     /gene="yciE"
                     /locus_tag="N7C74_004904"
     CDS             complement(547..1053)
                     /gene="yciE"
                     /locus_tag="N7C74_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGU2434583.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(1099..1584)
                     /gene="yciF"
                     /locus_tag="N7C74_004905"
     CDS             complement(1099..1584)
                     /gene="yciF"
                     /locus_tag="N7C74_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGU2434584.1"
                     /translation="MEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAFHAHLE
                     ETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAALIAAAQ
                     KVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNKKAENK
                     A"
BASE COUNT          426 a          319 c          329 g          512 t
ORIGIN      
        1 atgctcttgg gcgattttta gtgattgttt aaatagccct gtcatgcagt taaagtggcg
       61 ggcatcctct tcaaggatgg tgatcctcaa ccgcacccat ttaggatgcg actattgtgg
      121 tagaacaatg taaaccataa ttaatctgcg tgcggataca tgcttcatag ataggatatt
      181 ctggtttggg ttgtgaatgt gttggtcatc gagtggacaa taaggtaaga ggagtaactc
      241 ctgcaaaaca aaaaaagagt tcaacaaaat caatcagtta aaaacacgag agatctgtat
      301 aaaaaagcat cgtaaattct gtatttttcc tttaaatata atatgttatt gagttttgta
      361 aaatctacca gtgtggtaca tggatatcga taccaccgcc aattataaac tgggcggtgc
      421 acagcaacat gatagcgtac gcctcgatcc gtgggtgttt atgttctcag caggatatcg
      481 tttttaattc cgcacaaaaa cgaccccgta atatacgggg tcaataagga catggcataa
      541 agggtattat ttcttcgctt ctacgccatc agtttcagag cgaattaaaa atttctcagt
      601 tgtttgcgga atatgctgaa tcagccagtc ggccatttgc ttttcctcat ttaaaatcgc
      661 ttcgatgatc ggaattgagg ctgtatcacc ggcatttttt gctgctgcta atagagaggt
      721 gtaacaggcg atttcaaatt gctcgaagac atatccgcta atagagcctt tgactatttc
      781 atcagaaggg aatataccac cgattgactg cccaagcgca gccattttac tcatggaatc
      841 tttaatgact gaacgtgaaa tgtcattacg atcaagaata gtttccagtt gaacaatctg
      901 gtttttggtt tcactaagat gttgttcaat acgagcacgt agttcaggat aattatctat
      961 acggctggcc atggattcaa gcatagattc ggcttgcttt tccattgcat gggcgtcacg
     1021 taaccagtca tgataatgtt caatacgatt catgaaaatg tcctctttat aaaatgacca
     1081 aaagttaaag ttcatatttc aggctttatt ttcggctttc ttatttacgt tattaagggc
     1141 cagatcagtc agtttgatgt cggtggcctt ttcttcttcc agggtctctt tcagaagctt
     1201 cgctgcttta cggtaaccta attgttcagc cagcgtcgct aatgtcccgt aactggcaat
     1261 ctcataatgc tcgactttct gtgctgcggc aatcagtgcg gcatcacgca cttcgttttt
     1321 ctcggtactt tcgatgacct cattagcttc ttcaataaga ccttccattg ccacacattt
     1381 catgcgctta attttcagat tcgattctga ttccacaact tgatcaatac gttcaatctg
     1441 tccatgtgtt tcctcgaggt gcgcatgaaa agcctgactt aatttttcgt ttgatgttgc
     1501 tcttgcgagt tttgccagtg cccgggttaa ttgtttttct gcgctgtagg tatctgaaag
     1561 caggtgaata aatacatctt caatgg
//
LOCUS       DBFIFM010000397         1583 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.399, whole genome shotgun sequence.
ACCESSION   DBFIFM010000397 DBFIFM010000000
VERSION     DBFIFM010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.399"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(60..1288)
                     /locus_tag="N7C74_004906"
                     /pseudo
     CDS             complement(60..1288)
                     /locus_tag="N7C74_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
     gene            1380..1583
                     /locus_tag="N7C74_004907"
                     /pseudo
     CDS             1380..1583
                     /locus_tag="N7C74_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          349 a          450 c          378 g          406 t
ORIGIN      
        1 ttatgttttt cattatttgg ctgttgtctt tattacgata aatccatctg gatttgcccc
       61 tatatttcca gacatctgtt atcacttaac ccattacaag cccgctgccg cagatattcc
      121 cgtggcgagc gataacccag cgcactatgc ggatgccatt cgttataatg ctcgaacgcc
      181 tctgcaaggt tctttgctgc cgttaacccg tctggtttgg gcatgatact gatgtagtca
      241 cgctttatcg ttttcacgaa gttctctgct attccgttac tctccggact ccgcaccgcc
      301 gtgttcttcg gttcaagtcc caacatccgg gcgaactggc gtgtttcatt agcccggtag
      361 catgaaccat tatccgtcag ccactccact ggagacgacg gaagatcgtt gccgaagcgg
      421 cgttccaccg ctcccagcat gacgtcctgt actgtttcac tgttgaagcc gccggtagtc
      481 accgcccagt gcagtgcctc acgatcacag cagtccagcg cgaacgtgac acgcagtctc
      541 tctccgttat cacagcagaa ctcgaacccg tcagagcacc atcgctgatt gctttctttc
      601 acggccactc tgcctgtatg tgcccgtttc gatggcggta cagtaggttt tcgctcaagc
      661 aacagcgcat tctggcgcat gatccggtaa acacgtttgg cattgatcgc aggcatacca
      721 tcaagttctg cctgtctgcg aagcagcgcc catacccgac gataaccata cgtgggcagc
      781 tctccgataa catggtgtat acggagaagc acatccgtat catcagtgtg acgactgcgg
      841 cggccatcca tccagtcatc ggttcgtctg agaatgacgt acaactgcgc acgcgacacc
      901 cggagacaac ggctgactaa gcttactccc catccccggg caataagggc gcgtgcgcta
      961 tccacttttt tgcccgtcca tattcaacgg cttctttgag gagttcattt tccatcgttt
     1021 tcttgccgag caggcgctgg agttctttaa tctgcttcat ggcggcagca agttcagagg
     1081 caggaacaac ctgttctccg gcggcgacag cagtaagact tccttcctag tattgcttac
     1141 gccagagaaa taactggctg gctgctacac catgttgccg ggcaacgagg gagaccgtca
     1201 tccccggttc aaagctctgc tgaacaattg cgatcttttc ctgtgtggta cgccgtctgc
     1261 gtttctccgg ccctaagaca tcaatcatct gttctccaat gactagtcta aaaactagta
     1321 ttaagactat cacttattta agtgatactg gttgtctgga gattcagggg gccagtctag
     1381 tcatctacac aaccaacgcc atcgagtcgc tgaacagcgt aatccggcat gccatcaaaa
     1441 aacgcaaggt gttcccgacg gatgactcag tgaaaaaggt cgtgtggctg gcaatccagg
     1501 cggcttcaca gaaatggacg atgccactgc gggactggcg tatggcaatg agccgcttta
     1561 ttatcgagtt cggtgaccgc ctg
//
LOCUS       DBFIFM010000398         1565 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.400, whole genome shotgun sequence.
ACCESSION   DBFIFM010000398 DBFIFM010000000
VERSION     DBFIFM010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.400"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(66..1472)
                     /gene="gndA"
                     /locus_tag="N7C74_004908"
     CDS             complement(66..1472)
                     /gene="gndA"
                     /locus_tag="N7C74_004908"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HGU2434585.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQI
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT          393 a          393 c          378 g          401 t
ORIGIN      
        1 aattggcaaa aaaaagcccg gtacaatata cgccgggcct caattttatt gttgattaaa
       61 tcagattaat ccagccattc ggtatggaac acaccttctt tatcaatgcg cttataagta
      121 tgcgcaccga aatagtcacg ctgtgcctgg atcaggttcg caggcagaac agcggcgcgg
      181 tagctgtcat aataggcaac cgcagcggcg aaggtcggaa ccgggatacc gttctgtacc
      241 gcataagcga cgacatcgcg cagcgcctgc tggtagtcat cggcaatttg cttgaagtaa
      301 ggagccagca gcaggttagc gatctgcgga ttttcggcat aagcatcggt gattttctgc
      361 aggaactgcg cacggatgat gcagccagca cggaaaatct tcgcgatttc gccgtagttc
      421 agatcccagt tgtactcttc agacgccgca cgcagttgag agaacccctg agcgtaagaa
      481 acgattttgc ccagatacag tgcacggcga actttttcga tgaactcagc cttgtcgcca
      541 gctggctgcg cttgtgggcc agagagaact ttagacgcag caacgcgctg atctttcaga
      601 gaagagatgt atcgtgcaaa cacagactcg gtaatcagcg acagcggttc gccgagatcc
      661 agtgcgctct ggctggtcca tttgcccgta cctttgtttg ccgcttcatc caggatcaca
      721 tcaaccaggt agttaccgtc ttcatctttt ttagtgaaga tgtctttggt aatgtcgatc
      781 aggtagctgc tcagttcacc gttattccac tcggtaaaga tctgcgccag ttcttcgttg
      841 gtgaggttca gaccaccttt aagcagagaa taggcttcag caatcagctg catatcgccg
      901 tattcaatac cgttgtgaac catcttcaca tagtgacctg cgccatcggc accaatatag
      961 gtaacgcatg gctcaccgtc ttcagccact gcggcgattt tggtcaggat cggcgcaact
     1021 agttcatagg cttctttctg cccaccaggc ataatggaag gaccttttag tgcgccctcc
     1081 tcaccaccgg aaacaccggt accgatgaag ttaaagcctt ctgcagaaag ctcacggtta
     1141 cgacgaatgg tgtcctggaa gaaggtatta ccaccatcaa tgatgatgtc acctttatcg
     1201 aggtatggct taagggaatc aatagcagca tccgtgcctg cacctgcttt caccattaac
     1261 aagatgcgac gaggcgtttc cagagattca acaaattctt tcaccgtata gtaaggaacc
     1321 agtttcttgc ctggattttc ggcaatcact tcttccgtct tttcacggga acggttgaaa
     1381 atagagacgg tataaccacg gctttcgatg ttgagcgcaa ggttgcgccc catcactgcc
     1441 ataccgacta cgccgatctg ttgctttgac attgtttact cctgtcaggg gttaccgcta
     1501 gatatgcggc ttgaataacg tataaaaatg tatcagagta ttataaattt catgctgtat
     1561 cagta
//
LOCUS       DBFIFM010000399         1550 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.401, whole genome shotgun sequence.
ACCESSION   DBFIFM010000399 DBFIFM010000000
VERSION     DBFIFM010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.401"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            1..464
                     /locus_tag="N7C74_004909"
                     /pseudo
     CDS             1..464
                     /locus_tag="N7C74_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            461..787
                     /locus_tag="N7C74_004910"
     CDS             461..787
                     /locus_tag="N7C74_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HGU2434586.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDEMLMDKVRQATAVLLAYIQGSR
                     DKVISEDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
     gene            797..1147
                     /locus_tag="N7C74_004911"
     CDS             797..1147
                     /locus_tag="N7C74_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HGU2434587.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDHVLNVVGVPVPDAT
                     GGRLEILCRLGGEK"
     gene            1144..>1550
                     /locus_tag="N7C74_004912"
     CDS             1144..>1550
                     /locus_tag="N7C74_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HGU2434588.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRCSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIA"
BASE COUNT          326 a          365 c          538 g          321 t
ORIGIN      
        1 ctggaggcgg tgagtgccct gccggctccg caggacgggc gtgatgccac ggcactggaa
       61 atactccccg ccattgacga tcaaaaatcc tttccccggg gctcgtatgc cacacaccag
      121 ggtggactct ggcgggcgta tgaaaaaacg tacgggatgc ggggatggga atgcctggtt
      181 gacggggtgg cggatattga cgtcagcatg acgggtgaac ggtcgttctc tgtggtggtc
      241 cggcagagca gtggccagcg tacggaaaaa acattttccc tgccggtgat gctctaccgt
      301 ggtgtgttca gaatcggcga aacttaccac cccggcgata cggtgacgtg gggggctcgt
      361 tgtggcactg caacagtatg accggtgata agcccggaga agctcattca tcaggctgga
      421 ctctggctgc aaaacgtggg cgggatgcag gaggtggaaa gtgacagcat tactgacact
      481 ggaagaaatc aaggcacatc tgcgtgttga ccatgacgcg gatgatgaga tgctgatgga
      541 caaggttcgt caggctaccg ccgtgctgct ggcctacatc cagggcagcc gggataaggt
      601 gattagtgag gacggtgagc tgatccctgg tgaggcatta acccggatga agggggctgc
      661 catgcgactg accgggatgc tgtaccggaa tccggatctt gctgagcggg aagacctcgt
      721 gcagggggaa ctgccgtttt ctgtttccgt gctgatttac gatttgcgtt gtccgacggt
      781 gttatgagga gggggaatgg caatatctgc aggtcgtctg acacagatga tcagtgttct
      841 gaacccggtg ttaacccgta atgctgccgg agaaatgacg gaagaatggg tgtcatgcgg
      901 gaaaattcat gcggatatcc ggggcaggag cagccgggag cggatgcagt ccggtgcgga
      961 aatggcgcag gcggaaatcc gcatctgggt acgcggtcag tctggtcgtg aaatcacggc
     1021 ggcgtcacga cttcatgtgc tgagtggtcc ctggcgtgac catgtcctga atgtcgtcgg
     1081 ggtcccggtt ccggatgcaa ccggcgggcg tctggaaatt ctctgtcggc tgggagggga
     1141 aaaatgatcg aaaccctgct ggatttttcg gggctggagg acatcagccg cgatttgcag
     1201 cttctgagtg gtgcggaaaa taaccgggtg ctgcgtgagg caacccgtgc gggtgcgaat
     1261 gtgctgaaag aagaagtggt gtcacgggca ccggtacgca ggggaaaact tcgccgcaat
     1321 gtggtggtcc tttcccggtg ctcccgcgat ggcgggatgg aatccggtgt gcatatccgg
     1381 ggtgttaatc cggacaccgg taacagcgat aacaccatga aggcggataa cccgcgcaat
     1441 gctttctact ggcggtttgt ggaaatgggg accgtgaata tgccaccgca cccgtttgtg
     1501 cgcccggcat ttgatgtgcg cagtgaacag gcggcacagg tggcgattgc
//
LOCUS       DBFIFM010000400         1545 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.402, whole genome shotgun sequence.
ACCESSION   DBFIFM010000400 DBFIFM010000000
VERSION     DBFIFM010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.402"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..246
                     /locus_tag="N7C74_004913"
     CDS             <1..246
                     /locus_tag="N7C74_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HGU2434589.1"
                     /translation="LTYKSGKKGVRYLFECKDPESKAPKYIQFSDHIIAPRKSSHFHI
                     FMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            589..1119
                     /locus_tag="N7C74_004914"
     CDS             589..1119
                     /locus_tag="N7C74_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HGU2434590.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIVAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
BASE COUNT          478 a          333 c          283 g          451 t
ORIGIN      
        1 ctcacctata aatcaggcaa gaaaggcgtt cgttacttat tcgaatgtaa agatcctgaa
       61 agcaaagccc ctaaatatat acaatttagc gatcatataa ttgcaccacg aaaatcatct
      121 cattttcaca tttttatggg taatgattca cagcaatctt tattaaatga aatggaaaac
      181 tggccaacgt attatccata tcagttgagt agcgaagaag tggtcgagga aatgatgtct
      241 cattgagtat tctcatgata acgcctcgat gccgctttag tatgtcatca taactgccac
      301 tggtccatcc acaaacgcca ctgaacgcaa gctagctaca gacacgctca tcactatgac
      361 gtgtctgtat attaataagc taacccgcat tgagttaacc aataacggat tccatacaca
      421 atacggccaa cagagaaaga tacctgtgct cacgccattg cttatattgg ctggttacaa
      481 tgtgcactat caatttttta aataacaaat tcttaaaacc acatctgtat aaggaatttt
      541 taaggttcgt gggatagctt gactgtgaaa attacaggag ctacaaaaat gaaccgattc
      601 tcaaaaactc aaatttattt acattggata acgctgcttt tcgttgcaat aacctatgcc
      661 gcgatggaac tccgtggctg gtttcctaaa ggtagtagta cttatctgct gatgcgagaa
      721 acacattaca atgcgggtat attcgtttgg gtgttaatgt tttcacgcct gattataaaa
      781 caccgttata gtgatccttc tattgtgcca ccgccacctg cctggcaaat gaaagcggct
      841 tcgctaatgc acatcatgct ttatataacc ttccttgcgc ttcctctgct ggggattgct
      901 ttgatggctt acagtggaaa atcgtggagt ttccttggtt tcaatgtgtc tccctttgtt
      961 accccaaaca gcgaaattaa agcactgata aaaaatattc acgaaacctg ggcaaatata
     1021 ggctactttt taatcgtagc tcacgctggc gcagcactct ttcatcacta cattcagaaa
     1081 gataatactc tgttacgaat gatgcctcgc cgcaaataag tcgatcctct gacaaaggaa
     1141 atgtgaacca tggacatcaa cgcatcataa atgtttacgc aagtgcatac cgcactcttt
     1201 ccactatcaa caaggaggag cagaaaatgt gtaaatcgta caatggtaag acttatcaaa
     1261 aatacaggtt aattttggta tttaaacctt ttttacttgt tagtatgata caaaggcttt
     1321 caaaaaagct gggaaattca acaactcttg gaaattatgg actttgtccc gcggctttaa
     1381 tctgacggcc gcgttccttt tttgactcaa ttcttgaccc cttctctacg caacttcagt
     1441 tcccaccacc aactttgcgg cagtttcata ggatgaatgt ctaaaagaat aatggtgagc
     1501 gataagaaaa cgactgaata actgcacatt ttcgctcgaa agctt
//
LOCUS       DBFIFM010000401         1531 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.403, whole genome shotgun sequence.
ACCESSION   DBFIFM010000401 DBFIFM010000000
VERSION     DBFIFM010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.403"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            806..1408
                     /gene="fimB"
                     /locus_tag="N7C74_004915"
     CDS             806..1408
                     /gene="fimB"
                     /locus_tag="N7C74_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HGU2434591.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          459 a          260 c          328 g          484 t
ORIGIN      
        1 gagcttggtg taaatcagtg tttacccgcc atcaggctga gcataattct catcatgaaa
       61 tatgtttcct ggtttgtggc ttgtaactgg tcacttctga agtcgatctg gagaggctta
      121 ttgatgttgg tgttttcagg atgatgtttc acttagtttg tttgccgtat cgcccggcga
      181 atggctgtga ttgaggaagg tttaagtcgt agtgaccaaa gctatattta ccaacgaatg
      241 tagatgaaaa aatcatctcc tgcgttcccc catatctcca ggataaaaag gaatgtaaca
      301 atctcatggc gtaagctgac gaatcagcca ggaataatcg ctagggacct aagaattagc
      361 ttgataatag ccactaagaa attactgtgt tccatgaaat agccattttg tggcaaatgg
      421 agttgactaa taatgtcata tgtgagacgg ctagttgaac gaatattaaa ttttgctgaa
      481 ttttttatgt tgattttact tgttacagaa catatcacat gatatataga taagattagt
      541 tgcattaatg atgagggtta ttattagatt cgtatccgat tgataaatat ataaaggtac
      601 atagcatgca agagcatagc gtttgtatgg caacgttatt ataattaact gttgctactc
      661 catttaagtt cactcagaag aactggtcca cttacgttag ttattaagca aacgttcgct
      721 tttataaaca taatcaggat aaaaatgttg gattattgct aacctagcac agctagtgcg
      781 cgtctgtaat tataagggaa aaacgatgaa gaataaggct gataacaaaa aaaggaactt
      841 cctgacccat agtgaaatcg aatcactcct taaagcagca aataccgggc ctcatgcagc
      901 acgtaattat tgtctgactt tgctttgttt tattcatggt ttccgggcga gtgaaatttg
      961 tcgattgagg atttcggata ttgatcttaa ggcaaagtgt atatatatcc atcgattaaa
     1021 aaaaggcttt tcaacaacgc acccgctatt gaacaaagaa gttcaggctt taaaaaactg
     1081 gttgagtatc cgtacttctt atccgcatgc tgagagcgag tgggtatttt tatcgcgtaa
     1141 ggggaatccg ctttctcggc aacaatttta ccatattatc tcgacttccg gtggtaatgc
     1201 cgggttgtca ctggagattc atccgcacat gttacgccat tcgtgtggtt ttgctttggc
     1261 gaatatggga atagatacgc gacttatcca ggattatctt gggcatcgca atattcgtca
     1321 tactgtctgg tatactgcca gcaatgcagg gcgtttttac ggcatctggg atagagccag
     1381 aggacgacag cgtcacgctg ttttatagag atagttgaga taccagggat ggtgttaaaa
     1441 actacattat tcttacgata tatcaaaaat gattaaccat ttattaattg atgttattgt
     1501 aagtatgatt cttaattatc ataataaaca t
//
LOCUS       DBFIFM010000402         1507 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.404, whole genome shotgun sequence.
ACCESSION   DBFIFM010000402 DBFIFM010000000
VERSION     DBFIFM010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.404"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..779)
                     /locus_tag="N7C74_004916"
     CDS             complement(<1..779)
                     /locus_tag="N7C74_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU2434592.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAITAEGDVVVSGATEPDWYYVIVLAGQSNAMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSESTGASQDSARWGVGKPLYQDLISRTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLAQFRA"
     gene            756..899
                     /locus_tag="N7C74_004917"
                     /pseudo
     CDS             756..899
                     /locus_tag="N7C74_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1094..1261)
                     /locus_tag="N7C74_004918"
     CDS             complement(1094..1261)
                     /locus_tag="N7C74_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HGU2434593.1"
                     /translation="MTVKLRLTVAALLLFLVVMVDFTSRIMSVLADGVLVCGIVVLLW
                     PVIKRNSLHNA"
     gene            complement(1258..>1507)
                     /locus_tag="N7C74_004919"
     CDS             complement(1258..>1507)
                     /locus_tag="N7C74_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HGU2434594.1"
                     /translation="AFTSEINAISATIIGQLDTNFKTGRRAALREIEDVKHDTREAED
                     VLDVAVAIAEADGEIEPEERKVLEEIAGVLGLRLENHL"
BASE COUNT          365 a          455 c          357 g          330 t
ORIGIN      
        1 tcagcacgaa actgtgccag catggctgta aacagcgcag gttgctgtgc gtgggtggcg
       61 gcgctcatgt caaactctcc ctgcatccag cagacggcca gcagaacgtt tttgggattt
      121 ttctgcaatg ccgctttggt gcgggaaatc agatcctgat ataacggctt gcccaccccc
      181 cagcgtgccg aatcctggct ggcccccgtg gactcgctga atgtcccctc cgcgccctgg
      241 gtaaatgccg aaccaccacg acagcatggt accagcagga tccccgcgtt attcgggata
      301 tacgggagca gttttttggc aatatgtaac ccctggccga cacagccgta ctgccctttg
      361 ctcaggtctg ccttcggatg attcagcgta ctcatatcct gcacatcatg caggcagtgg
      421 tcggccggaa taatatcgtt atatctgcag gcagccccac ccggcgtcac tgtactgcgg
      481 cgcgccagct gtttaatgcg cggatccgga gcatcgtatg aatccggcag cggaagccct
      541 tcaccgtaag ccatggcatt ggactgcccg gccagtacga tgacgtagta ccaatccggc
      601 tcagttgcac cactgaccac cacatcacct tctgctgtaa tcgcctgcat cagggtataa
      661 ggggttatgg ccaccggact accaaacggc tgccagccct ctttcagttt gtgtgtcagc
      721 ttttccgcaa ggtctgacgg cgacgccgcc ctgacaacat cataatgttt aatcgacatc
      781 gaatttctcc cgtgtacagg aacagagtta aaaagccgga accggaatca aatcacagga
      841 tgaccatctg ccagtggctg gtcgtaaaaa aaaggccgcg ccatgcgcag ccggaaataa
      901 agggataacg atgatagttt gagaaaaaca gaaataacac ttttgtggca aagcatggtg
      961 ccgggtgcct cccggtgaat tcagtaccag cacctgaatc cgcgattatc ccatatacct
     1021 tcttgctgat cgcccctccg cacaggggga ttcaccatgc agaagtgttt ttaataaaca
     1081 gcaaacaaaa aaatcaagca ttatgcaggc tgtttctttt tatcaccggc cacagcaata
     1141 ccacaatgcc gcagaccagc accccatccg ccagcaccga catgattctg ctggtgaaat
     1201 ccaccatcac caccagaaac agcaggagtg cagccacagt caggcgcagt tttaccgtca
     1261 caggtgattc tccagacgaa gccccagaac accggcaatc tcttccagca ccttgcgctc
     1321 ttccggctca atttcgccgt ctgcctccgc aatggccacc gccacatcca gcacatcttc
     1381 cgcttcacgc gtatcgtgtt tcacatcctc gatctcacgt aacgccgcac gacgaccagt
     1441 tttaaagttc gtatccagct gaccgataat ggttgcgcta atcgcattaa tttctgacgt
     1501 aaacgcg
//
LOCUS       DBFIFM010000403         1489 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.405, whole genome shotgun sequence.
ACCESSION   DBFIFM010000403 DBFIFM010000000
VERSION     DBFIFM010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.405"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            36..1265
                     /locus_tag="N7C74_004920"
     CDS             36..1265
                     /locus_tag="N7C74_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU2434595.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT          375 a          321 c          304 g          489 t
ORIGIN      
        1 cattttgttt aatttgtaga tgatacgttc agataatgtc taatatttgg tctaaagaag
       61 aaactctgtg gagtttcgcg ctctacggca cagccgttgg tgcaggcacg ctcttccttc
      121 ctattcagtt aggttcggca ggggctgtgg tcctgtttat tactgctctg gtcgcctggc
      181 ctttaacata ctggccacat aaagctttat gccagttcat cctctcatcg aaaacatcag
      241 caggtgaagg gataacgggc gcggtaacac actactatgg caagaagatt ggtaatctga
      301 ttaccacgct gtacttcatc gccttttttg tcgtcgtgtt gatatatgca gtggcaatta
      361 ccaactcact tacggaacag ctggcaaagc atatggttat tgatcttcgc atccgtatgt
      421 tggtgagtct gggtgttgta ttaattctga atctcatttt tctgatggga cgtcatgcca
      481 ctattcgggt aatgggattt ttggtattcc cattgattgc ctatttctta tttctttcca
      541 tttaccttgt aggtagttgg caacctgatc tattaacaac ccaggtagag ttcaatcaga
      601 atacccttca ccagatatgg atatcgattc ccgtgatggt tttcgccttt agccatacgc
      661 ccattatttc tacgtttgcc atagacagac gtgaaaaata tggcgaacac gctatggata
      721 aatgcaaaaa aattatgaaa gtcgcttatc tcatcatctg cataagtgta ctgttctttg
      781 tctttagctg cctgctttct attccacctt cgtatattga agcggctaaa gaagaagggg
      841 tcaccatttt atcggcgctt tctatgctgc cgaacgcccc agcatggttg tcaatttccg
      901 ggattattgt cgcagtagtt gcgatgtcga aatcattcct gggtacgtac tttggcgtta
      961 ttgaaggtgc cacggaggtc gtcaaaacaa cactacagca ggttggtgta aagaaaagtc
     1021 gtgcatttaa ccgcgcacta tcaattatgt tggtatcgct gattaccttc attgtttgtt
     1081 gcattaaccc gaacgcgatt tcgatgattt atgcgatcag cggcccgctc attgccatga
     1141 tacttttcat catgcctacg ctatcaacat atctcatccc ggcgcttaaa ccctggcgct
     1201 ccatcggaaa tctgattacg ctgatcgtgg gtatcctgtg cgtatcggta atgttcttta
     1261 gctaatatcc attgtgaatg gttcaaaatt gctacaattt tgaaccattc cagttttcat
     1321 aatgtgcatt tgttattatt ccgctctatc acttctctcg tcatatccta gtattatcag
     1381 ttagttaaac acacaaagag atctgaacat cttaaataag actatttaag atgcataact
     1441 tagattcgca agatatcttc tggtcattca aaaacaattt gcgatatag
//
LOCUS       DBFIFM010000404         1471 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.406, whole genome shotgun sequence.
ACCESSION   DBFIFM010000404 DBFIFM010000000
VERSION     DBFIFM010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.406"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..474
                     /locus_tag="N7C74_004921"
     CDS             <1..474
                     /locus_tag="N7C74_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU2434596.1"
                     /translation="FEECAAMAERIEEMQTKSAPDSFGIIGENIRTQDNRITSDPMFC
                     VYQKREIAVDADYDHDRIVWVDEDGNEANKRHSRRLELLHENFREPPEKWRRVAVKDI
                     DEFVTCCFTEQGCKDYLAVNGHNLRLPFIYVKSGFRNAEYIGIRNWLAGIRIKGE"
     gene            476..652
                     /locus_tag="N7C74_004922"
     CDS             476..652
                     /locus_tag="N7C74_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434597.1"
                     /translation="MALTHRELCQIAYKFLKRNGFKVCFHDRFIAVTSTGEQPDAMGF
                     RNSASCLIGEMFSC"
     gene            986..1342
                     /locus_tag="N7C74_004923"
     CDS             986..1342
                     /locus_tag="N7C74_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434598.1"
                     /translation="MTTITDKELIKEIKERIGSLDVRDNIERRAYEIALASLEAEPIA
                     WECGENIILFNPDTVEAYAKRAEISPKPLFSAPPALVVPDKLPREYRNGWPLAYSDYA
                     EGWNDCREAMLQGDKS"
     gene            1339..>1471
                     /locus_tag="N7C74_004924"
     CDS             1339..>1471
                     /locus_tag="N7C74_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434599.1"
                     /translation="MINRIKLEHILEYARQQRHIGQHCKIPPGDMVEIMEIAMRKAGN
                     "
BASE COUNT          432 a          292 c          378 g          369 t
ORIGIN      
        1 tttgaagagt gtgctgcgat ggctgaacgt atcgaagaga tgcagacaaa atctgcacca
       61 gattcgtttg gcatcatcgg tgaaaatatt cgaacacagg acaatcgaat aacgtcagat
      121 cccatgtttt gtgtgtatca aaagcgcgaa atcgctgttg atgctgatta tgaccatgac
      181 cggattgtct gggttgacga agatggcaat gaagccaata aacgccatag tcgtcgtctc
      241 gagctacttc atgaaaactt tcgagagcca ccagaaaaat ggcggcgcgt tgctgtgaaa
      301 gatattgatg aattcgttac ctgctgtttc accgaacagg gttgtaaaga ctacctggca
      361 gtcaatggtc acaatcttcg cttgccattt atatatgtaa aaagcggttt caggaacgct
      421 gaatatatcg gcataagaaa ctggcttgct ggcattcgca tcaaaggaga gtgatatggc
      481 gttaacacac cgcgaactct gtcagattgc gtacaagttc cttaagcgca acggtttcaa
      541 ggtttgtttt catgaccgct ttatagctgt aaccagtacc ggagaacagc cagatgctat
      601 gggattcaga aattcagcat catgcctgat tggcgaaatg ttctcgtgct gacttgttgg
      661 cagatagaaa aaagcgtttt cgtaaaaatc cgtctcttgg aatgggcgac tggcgattct
      721 ttattagtga gccgggaatt atttcaattg aggatttacc acctggctgg ggattacttc
      781 acgttgttaa cggaagagta cggaaagtac atgggtggcc caagggtaat tgctgttggg
      841 gtaatcctga cgataagcca tttactggaa ataagcaggt tgaatgcgat tacatgttgt
      901 ctgcattaag gcgcatggag ttgagagggc accttaatga aatatatgac ggtgtaattg
      961 ttaataagaa agaaggaaac gcggcatgac cactattacc gacaaagaac tgattaaaga
     1021 aatcaaagag cgcataggca gcctggacgt tcgagacaat attgagcgcc gtgcttatga
     1081 aattgcactg gcatcgctgg aagcagaacc gatagcgtgg gaatgcggtg aaaacataat
     1141 cctgtttaac cctgacacag ttgaagcata cgcaaaacgt gcggaaatat cacctaaacc
     1201 actattctcc gcgccgccag cgctggtagt gcctgataag ttgccgcgtg aatacagaaa
     1261 cggttggcct cttgcgtata gtgattatgc tgaaggctgg aacgactgcc gcgaagccat
     1321 gcttcaggga gataaatcat gattaatcgt atcaagctgg agcacatcct cgaatatgcc
     1381 aggcagcaga ggcatattgg tcagcattgt aaaattccac caggagatat ggttgaaatc
     1441 atggagattg ccatgcgcaa ggctggcaac t
//
LOCUS       DBFIFM010000405         1469 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.407, whole genome shotgun sequence.
ACCESSION   DBFIFM010000405 DBFIFM010000000
VERSION     DBFIFM010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.407"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            157..309
                     /gene="hokA"
                     /locus_tag="N7C74_004925"
     CDS             157..309
                     /gene="hokA"
                     /locus_tag="N7C74_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HGU2434600.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(286..357)
                     /locus_tag="N7C74_004926"
     CDS             complement(286..357)
                     /locus_tag="N7C74_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434601.1"
                     /translation="MRNAVRKAGITFRRRLLLFQIAG"
     gene            complement(497..709)
                     /gene="cspA"
                     /locus_tag="N7C74_004927"
     CDS             complement(497..709)
                     /gene="cspA"
                     /locus_tag="N7C74_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HGU2434602.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(990..1280)
                     /gene="yiaG"
                     /locus_tag="N7C74_004928"
     CDS             complement(990..1280)
                     /gene="yiaG"
                     /locus_tag="N7C74_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HGU2434603.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
BASE COUNT          399 a          315 c          319 g          436 t
ORIGIN      
        1 tagccacggg tgcttgagac tgtttgtctc aggtattcac cgaaaggcag acagagaaaa
       61 gccccacctg actataaatc aaagtgaggc ccctctatgt atagacacca taaggttagc
      121 ctcttactcg ttggaaatca acacaggggg ctgggaatgc cgcagaaata tagattgctt
      181 tctttaatag tgatttgttt cacgctttta tttttcacct ggatgataag agattcactg
      241 tgtgaattgc atattaaaca ggggagttat gagctggcgg catttttagc ctgcaatttg
      301 aaagagtaag agtcttcggc ggaaagttat tcccgccttt cttacggcgt tgcgcattct
      361 cattgcaccc aaatttattt ttcacaaaaa taatactaga ttttattacg cgatcgatta
      421 tttatttcct gaaaaacaaa taaaaaaatc cccgccaaat ggcagggatc ttagattctg
      481 tgcttttaag cagagattac aggctggtta cgttgccagc tgccgggcct ttagcgccgc
      541 tttcgatggt gaaggacact ttctgacctt cgtccagaga tttgtaacca tcgttctgga
      601 tagcagagaa gtgtacgaac acgtctttag agccatcgtc aggagtgatg aagccgaagc
      661 ctttgtcagc gttgaaccat tttacgatac cagtcatttt accggacata gtgtattacc
      721 tttaataatt aagtgtgcct ttcggcgata tggcgtgctt tacagatttt gaagcgttaa
      781 aggaatgtgc actacgaggg gtatcaacga taactcttga agggacttgc cttactacac
      841 tgctttaatg gtctgtacgt caaaccgttg atgtgcatta agccacgcat tggcgggtga
      901 tgcaacaatt atttttcata tttatgatta atcggttgga aaacggtcct gtcatcagga
      961 ccgtaaacag caataaagtg gataaaagtc tattccatca actgcttact taatgccggg
     1021 ttggcttgaa tcaaacgcat caattttagt tcggcacttg aaggcttcac gcgtctggat
     1081 tcccattcct ttaccatggc gactgatacg cccaaaaccc gggcgaaatc atcgattttt
     1141 aatcctgtcc cttttcgtaa ctgctcaatt tcggtacagg acgttgttct gtgcgtcagg
     1201 gtaattttct gcgtttcatc tttaaaaaca atctgttcca ggctgctcaa cagctcatgc
     1261 attggatctt tatattccat tgaaaactcc tcaaatcaca ctgcgggatc gtgaattgca
     1321 tcgaagctca ttaagaatag tcgggaaaag cagacacagc ggggtgaagt cagggcatgc
     1381 tctggctatg attcgattca gccagtcatt tagcgtgact tatagccacc taacgatctg
     1441 ataaatgccg atatatctct aaccactcc
//
LOCUS       DBFIFM010000406         1433 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.408, whole genome shotgun sequence.
ACCESSION   DBFIFM010000406 DBFIFM010000000
VERSION     DBFIFM010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.408"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..820)
                     /locus_tag="N7C74_004929"
     CDS             complement(<1..820)
                     /locus_tag="N7C74_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU2434604.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKA"
     gene            complement(817..1023)
                     /locus_tag="N7C74_004930"
     CDS             complement(817..1023)
                     /locus_tag="N7C74_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HGU2434605.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            complement(1007..>1433)
                     /locus_tag="N7C74_004931"
     CDS             complement(1007..>1433)
                     /locus_tag="N7C74_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HGU2434606.1"
                     /translation="MPKTRNQRGVYLCEVGTDTAKEILYARMKADPSPADEATSYAIR
                     FPDDPEIFSQTEAQQLVAEELVEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSV
                     QRWQLDLAVLAKSREEETTRPTLKELAAKLSGGVNGYSR"
BASE COUNT          294 a          478 c          344 g          317 t
ORIGIN      
        1 tggctttcac aatggccgac tgaagctgtg ttgcctgcag ggaatcgagc atcttcagcc
       61 gttccatgac gctgtaaaac tggttggccc cacgggtctg cccgtcctcc accggctcga
      121 aaatatgcag catggccgga cgcccggtgg gaagttcacg cgggatccgt tcccatcgtc
      181 cactcccgga gcgaggaaaa tcatcctcac agatatggta cgcaacggca cggccatatc
      241 gatcgacctc caccccggcc cgcagaaaac ggttccccat accgtgtcct ggcgtgtcca
      301 cccgtttcgg actcacggct ttaaaacgcg tacggaataa ctgcgtggtt tccgtatccc
      361 agaccggctg cacaaagatt tcgccgttaa acgcatgaac gcccacacct tcacggataa
      421 attccgtgaa cgtgcgtttt ccttccacgt cgatctcgcc agacatccct tcggcgtatt
      481 ccgaccaggc cgcctccacc tcatcgacaa agctttttgc tgcggtctcc cgcatcccca
      541 gccagcgcca gttcggacgg tagctgataa gaaacatatg cccgacaatg tgatccttat
      601 gcagtgccac cgcattggcc gctattccgt tattgcgcac cagatcatct gcccgggcat
      661 tccccagacg caacgcgggc agcagggccg catcggcact ctgcgccggt ggcaaccact
      721 ccgccatttg cccgccaaat cctgcaccgc ccccgttgta gctgagactc tcacgaagcg
      781 gaacgccgtt cacatcaatc aggacaggcg ttcgtttcat aacctcactc ccagcggacg
      841 acggcgacgc cgggttgtcc ccagtaccga ctccgcatca ttgatcgccc ggttaagctc
      901 atccagagaa gccgccgtat attcaattct gcgaccatct ttctggacag acaccacccg
      961 tttaccggtt aataaatcaa ggcgcgcctg acgcagcgcc tgcagttcag cgactgtaac
     1021 cattcactcc tccggacagc ttcgctgcca gttctttcag ggttggccgg gtcgtctctt
     1081 cttcccggga ttttgccagt acagccagat caagctgcca gcgttgcacg gacacacgta
     1141 atgccgcgta ggcatacacc aggcagtcca gcgcttcgtt acgccgcttt ttgttatccc
     1201 acagcagacg catctttcct ttttcccact tctccaccag ctcttccgcc accagttgct
     1261 gcgcctctgt ctgcgaaaaa atctccggat catcaggaaa acggatggca tacgacgtgg
     1321 cttcatccgc aggcgaggga tcggctttca tacgggcata gagaatttct tttgcggtgt
     1381 ccgttcccac ttcacacaga tacacgcccc gctgattgcg ggtttttggc atg
//
LOCUS       DBFIFM010000407         1431 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.409, whole genome shotgun sequence.
ACCESSION   DBFIFM010000407 DBFIFM010000000
VERSION     DBFIFM010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.409"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..>1431
                     /locus_tag="N7C74_004932"
     CDS             <1..>1431
                     /locus_tag="N7C74_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU2434607.1"
                     /translation="GQYGTVGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADG
                     TYSDASGASENSTRWGVDKPLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPA
                     NHAAQFGALVDKFRADLADMAGQCVGGSADGVPWICGDTTYFWKQKNEATYQTVYGSY
                     KNKTEKNIHFVPFMTDENGVNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASH
                     FSAWARRGIISDRLATAILRHAGRVALNAGASSTVSEVRPSSPSGAEATGITTLLSYL
                     ASESEGSLKVQGWSASGGRAEVVSDAEGTGGKAVKLTKEAGKSSWVLEYAAGNGAALL
                     QKGGQIRCRFKVSGALAANQYVMAFYWPVSSLPQGVALTGDGGNNLLAAFYIQTDAKD
                     LNVMYHNAKVATNNLKLGSFGAFDNEWHALAFRFAGNNSLQVTPVIDGQDGTPFTLTQ
                     SPVSAFAADKLHVTDITRNATYPVLI"
BASE COUNT          344 a          342 c          459 g          286 t
ORIGIN      
        1 ggggcagtac ggaaccgtgg ggcaggggct gcatatcgcc aaaaaattgc tgccgtttat
       61 accggcgaat gcgggcattc tgctggttcc gtgctgtcgt ggtggttcag cgttcaccac
      121 cggagctgat ggcacataca gtgacgcgag tggtgcttcg gagaattcaa cccgctgggg
      181 tgtggacaag ccgctgtata aggaccttat cggtcgaaca aaagcagcac tgaagaagaa
      241 cccgaaaaat gtgctgtttg ccgtggtgtg gatgcagggg gaatttgatt ttggcggtac
      301 gccggcaaat cacgcagcac agtttggtgc gctggttgat aaattccgtg cagacctggc
      361 ggatatggca ggtcagtgcg tcggtggctc tgctgacggt gttccctgga tatgcgggga
      421 cacgacgtat ttctggaagc agaagaacga agccacctac cagacggtgt acggcagcta
      481 caaaaacaaa acggaaaaaa atatccattt cgtaccgttc atgacggatg agaacggggt
      541 gaatgtgccg acgaacaaac cggaagaaga cccggacatt ccgggtatcg gatattacgg
      601 ttcgaaatgg cgtgacagct cagccacctg gacgtcacag gacagggcga gccatttcag
      661 cgcctgggca cgccgtggga ttatttccga ccgtctggca acggcgattt tgcgccatgc
      721 gggaagagtg gcgctaaacg cgggggcatc atcgacagta tcagaggtgc gcccgtcatc
      781 gccttccggt gcagaagcca caggcatcac aacactgctc tcttaccttg ccagcgagtc
      841 agagggaagc ctgaaagtac agggatggtc agccagtggc ggcagggcag aagtggtcag
      901 cgatgcggag ggaaccggag gtaaggcagt gaagctgacc aaggaagccg gtaaaagcag
      961 ctgggtgctg gagtacgccg cgggcaacgg tgcggctctg ttacagaaag gggggcagat
     1021 tcgctgccgc tttaaggttt cgggagcgct ggctgcgaac cagtatgtta tggcgtttta
     1081 ctggccggta tcttcactgc cacagggcgt tgccctgacc ggagacgggg ggaataacct
     1141 gctggcagcg ttctacatcc agacagatgc aaaagacctg aatgtgatgt accacaatgc
     1201 gaaagtggcg acaaacaacc tgaaactggg aagctttggc gcatttgata acgaatggca
     1261 tgcgctggct ttccgctttg ccgggaataa cagccttcag gtgacgccgg ttattgatgg
     1321 tcaggatggt acaccgttca cgctgacgca gtcaccggtc agtgcctttg cggcggataa
     1381 actgcatgtg acagacatta ccaggaatgc gacttacccg gtgctgattg a
//
LOCUS       DBFIFM010000408         1411 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.410, whole genome shotgun sequence.
ACCESSION   DBFIFM010000408 DBFIFM010000000
VERSION     DBFIFM010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.410"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(383..955)
                     /gene="hdeD"
                     /locus_tag="N7C74_004933"
     CDS             complement(383..955)
                     /gene="hdeD"
                     /locus_tag="N7C74_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HGU2434608.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            1210..>1411
                     /gene="hdeA"
                     /locus_tag="N7C74_004934"
     CDS             1210..>1411
                     /gene="hdeA"
                     /locus_tag="N7C74_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HGU2434609.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPE"
BASE COUNT          476 a          307 c          279 g          349 t
ORIGIN      
        1 atgtcttcag gtttttatat agcgtctgga gacatggata tttatgcaat gtaatcaacg
       61 ccagttgcaa aaaaatgaac ccggattact tatttaaccg cccatagcat caacgcttac
      121 ggaactcttt gttcactttc ccacagcact tttaaaacat taaacctgac tacggaaaat
      181 atcagccatg ataatgtttg aaatggctaa tttgccatgg agtgaaaaaa attagatgaa
      241 attcagtagg ttgaaataat cactagcagg taattatttc aatgatagtg cgcaattgat
      301 ctacaacact gcgtagcgga gagagtatta atcggatcat agtcacatca agtgactatg
      361 atccgggtga caaccgggat aattattgct gcttaacgaa caaactggcg aagctgaaca
      421 ggctggcggc gctaaatatc agttcaattc ccaccagtgt ggaaaccagc gttacagaca
      481 ccatcggcgt tgcaccaagg aatatccagg caatgacgat atccagcaca ccaataacga
      541 gctgtagcca gctgcctttc attgaacgct gacgatacca actcatcagg cgaataaccc
      601 ctgcaacaca gaacaaaccg gcaataaatg ccgcaatggc aaaaatgccc agctccggtg
      661 cgcggatgaa gaaatagccg atcaataaat aggcgactgc gacgaggaaa ccggataata
      721 ccggccagaa attatgactg cggttgctga ataatccaac aataagcgca atgcccgaac
      781 agattaataa tgcacctact actgtgctta aaatatcgcc agaaacgaac gggaaactga
      841 tacacagcaa cccgacgata aacagcagca cggcaataaa ctggattgct ctgcgatgct
      901 ttttcagcat ctccagatca aacttcaaaa ttgttgcctt atctatatat aacatagaac
      961 caccctataa aattaagaag aaaatcccct gctatcaatc tatgccaaaa acgcgtctaa
     1021 gaatgcagtc gatttaataa aaatttccta attgcagtat ctgatgcatc tgtaactcat
     1081 tgtattgaaa taaaaatatc tgattttgat attttccatc aacatgacat atacagaaaa
     1141 ccaggttata acctcagtgt cgaaattgat tcgtgacggc tctttcactt tatagttgag
     1201 gatattacga tgaaaaaagt attaggcgtt attcttggtg gtctgcttct tctgccagtt
     1261 gtgagcaatg cagcggatgc gcaaaaagca gctgataaca aaaaaccggt caactcctgg
     1321 acctgtgaag atttcctggc tgtggacgaa tccttccagc caactgcagt tggttttgct
     1381 gaagcgctga acaacaaaga taaaccagaa g
//
LOCUS       DBFIFM010000409         1389 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.411, whole genome shotgun sequence.
ACCESSION   DBFIFM010000409 DBFIFM010000000
VERSION     DBFIFM010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.411"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            82..345
                     /locus_tag="N7C74_004935"
     CDS             82..345
                     /locus_tag="N7C74_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HGU2434610.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
     gene            647..787
                     /locus_tag="N7C74_004936"
     CDS             647..787
                     /locus_tag="N7C74_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU2434611.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
BASE COUNT          383 a          293 c          337 g          376 t
ORIGIN      
        1 gttataagaa cagacagttg aatctgaccg tctgaatatg tacctttatg cggaattttc
       61 acttaaaggg ggaaaactgt tgtgaaaccg aatatccatc cggaataccg tacagtagta
      121 tttcacgata ccagtattga tgagtacttc aggatcggtt cgacgatcaa aactgaccgg
      181 gtaattgaac tggatggtgt aacttaccca tatgttacaa ttgacgtttc ttctaagtcg
      241 catccgtttt atacaggtaa actgagaact gtcgcttcag aaggtaatgt tgcacgcttt
      301 acccagcgtt ttgggcgttt tgttgatgcg aaaaaaggtg gctgattttg ccgccagggt
      361 gcgtttgaca atttaaaaac gcaccagaca gagagatcct gcacctgaat ataccgttag
      421 cccgtcccac cggggcgtgt gcagatgatc tcgtcaccgg aagattgcgg acctctctga
      481 aaaccagtac attacgcgcg ggtgcctttc ggctgacggc tggaaggatt acctgaaggc
      541 cggatgtgga aaggccccga gtcaacttaa acgttaaccc gaggccctga ccatctacct
      601 tcagcaagtg gaaggttagc gcctctctgt aaaaggagca agtgctatgc cgcaaaaaac
      661 gatcattgtg ggcatgttat gcctgaccat gttgctgacc gtctgggtgt tacacgcatc
      721 accgtgtgaa ttcagggtaa gcttcatgtg gtcggaaatt gcggcgttct tgcagtgtaa
      781 accgtaagaa accgcagcgg ggacaacagt ccccgctatc cggttgccaa aggttcggtc
      841 gaaagtcgca cccttgcaca caaatcacaa agaaaaaaca aaagtccgca gtgtaaaaac
      901 tgtgggcttt tttattcgca tcccgaaagt gtggtaacag cgtgggggca ccaaaaaaag
      961 ccccataaaa tggggctgaa gattctgtaa ggaatttcac agcaatgaga ttcctgcttt
     1021 ctccaaggat atacgtgtct tcaggctgaa tcaatggggg cgtggctctg aatccacctg
     1081 cgattaaaac ttgcacaatg ccgggagttg agttagaaaa gtacagggcg cgatatccgg
     1141 cttattttgt tgctgtgttt tgcctcgtgc tggatgtcgc gtccaccgtt tttctgatga
     1201 ttcatatgaa atggtacttt attgatttac tgccattctg atttgccttt ttatgttcat
     1261 tattgcggtt gtcacttcca gtttctggtg atgttttgac aaaagtgaaa gaataaaacg
     1321 ataattgcag aaggtattgc taaaactata catatcatta tataaacaag atgaaaatga
     1381 tatatacca
//
LOCUS       DBFIFM010000410         1379 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.412, whole genome shotgun sequence.
ACCESSION   DBFIFM010000410 DBFIFM010000000
VERSION     DBFIFM010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.412"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..224)
                     /locus_tag="N7C74_004937"
     CDS             complement(<1..224)
                     /locus_tag="N7C74_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HGU2434612.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTL"
     gene            complement(239..955)
                     /locus_tag="N7C74_004938"
     CDS             complement(239..955)
                     /locus_tag="N7C74_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HGU2434613.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            complement(1021..1365)
                     /locus_tag="N7C74_004939"
     CDS             complement(1021..1365)
                     /locus_tag="N7C74_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HGU2434614.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
BASE COUNT          311 a          440 c          329 g          299 t
ORIGIN      
        1 aacagcgtca catccgcttc caggttacgg cgggttttcg ccaccaccga cagggtatcg
       61 tcatcctctc catcaccatt gagcacttcc tgccacagat accaggcctc tgccgaaggc
      121 tcccgcagca ccacgctgac attaccccat tccggcacct tcaccgtttt atgacgaaac
      181 cctgacagtc tggccagcgc cagcgttttc agatcctttt tcatgatgac ccatcccctt
      241 atccggcggc tgcgctcact gtcacggtgc attcaacaga cgtcacactc tgtgctttct
      301 ctgccgaatc ggtcaccaca caggtatatt tccccgcatc agcggactgc gcacctggct
      361 tactgaaggt gtctgtcgtc tgcccgtcaa ccggctgacc atccttcttc caggcgtatt
      421 tatacggcgg cgttcccccg ttggcactga ctgacattgt cagcagcgca ccggtattca
      481 cggtaagtgt cttatccaga tttttcacaa acgccagcgg taccacaaag gacaccggtt
      541 tgcctttcag acgcagtgag aacgttgcag ccaccacgcc gttggtaccg gatgaccagg
      601 tgtgctggcg cacttccgcc aggaatttaa agcccttacc ggacggaaac agcaccttaa
      661 acgcatacaa cgcgtcattg tcataggcat cacgcagggc gttctgggcc tgattcagat
      721 aaaaattgcc cgacatggaa atctcagacg acgcccccag accattgatg ttctcctgct
      781 ctgtggagca gagcgtggtc acatcaatat cctgtttctg cccggcggtg aactgcactt
      841 ccttgatggt gcagtccagg cgcagatatt ccgccttatc catagtttca gcagtcgccg
      901 gggcagatga aatcatcacc tgcgtcagct gtgagcgttc atacaaagca gacattctgc
      961 ctcctgataa taaaaaaccc gcacgcggcg gggtatgggt tttgtagaaa aaagaaaaag
     1021 tcacaccgtg acctgaaact ccagggttgc acggtaacag cggttttccg gaatatagtc
     1081 ctgcatttca ctgacggatc ccggggccag cagcattatg gcttcacggg cgtcctgacg
     1141 tatctgacgc gcctgcgtca cagtcccggc ataaacgtct atctgcaccg acactgagga
     1201 ctccgcctgc ccgcccatca cgtccgctga caccgatgaa atcaggctga aaaccaccca
     1261 cggaagcgcc accgacggcc tgccatccag cagggggacc acatacgggt acacctgccc
     1321 gccggcaaga tgcgccagat gaggatacaa atccgcctcc gtcatcgtct cagtacctc
//
LOCUS       DBFIFM010000411         1378 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.413, whole genome shotgun sequence.
ACCESSION   DBFIFM010000411 DBFIFM010000000
VERSION     DBFIFM010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.413"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..403)
                     /locus_tag="N7C74_004940"
     CDS             complement(<1..403)
                     /locus_tag="N7C74_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434615.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAI"
     gene            complement(656..1279)
                     /locus_tag="N7C74_004941"
     CDS             complement(656..1279)
                     /locus_tag="N7C74_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGU2434616.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAVFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGIVAEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAITR"
     gene            complement(1276..>1378)
                     /locus_tag="N7C74_004942"
     CDS             complement(1276..>1378)
                     /locus_tag="N7C74_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434617.1"
                     /translation="AVKPLKFANQMYIDTGAVFCGNLTLIQVQGEGA"
BASE COUNT          401 a          351 c          272 g          354 t
ORIGIN      
        1 caatcgctgc tgacagtagc aaaagaggta cctgaatctt ctgtctcaat atagaaaact
       61 caataattgc cggcacaaac aataattcca cagcaaaata aaggcgaaat acatttagct
      121 cttgcataga atgttttctt ttcactgcga aaaagaatac aacaccaata ccccaaccga
      181 taagaaatat agcaatgacg ataactgcaa aaaataaact tctggcaaca tcatcaacac
      241 ctgcacctac aatccaccat gggaagccgt agtaaaaaga agtaccccat ccatagaaat
      301 aagcactccc ccatccaagg catcccatgt aggcaataaa aagtgaagaa ctcctgagca
      361 gcgcaccatc cttcataacc accccaatac aagatgataa cattggctta caactcataa
      421 caaaagcaat tcaatgccgt caagaggtta caggctaaaa aaactctatt acattgcagt
      481 cagcatgttt actacacaaa tacaattcag agcataaaaa ctactcggcg gcaggttatt
      541 gagactcatc aatgacatgt aaaaaacgcc cattattggt gtcaagtttc cccaaagtta
      601 ttcaaaaagt caatattatg ccgttaatat gttgccatcc gtggcaatca tggcgctaac
      661 gtgtgatcgc attcaaaatg ttgtctgcga ttgactcttc cttgtggcat tgcacaacca
      721 gagcgtcata cagcggctta acagtgcgtg accaggtggg ttgggtaagg tttgggatta
      781 gcatcgtcac agcgcgatat gcggcgcttg ctggcattct tgaataaccg acgcctttac
      841 atcttccgca ctctttctca gcaactatcc cccactgctc tgttttggct atatcaaccg
      901 cacggcctgt accgtggcaa tctctgcatc ttgcgcccgg cgtagcggca ctacggcaat
      961 aatccgcata agcgaatgtt gcgagcactt gcagtacctt tgccttagta tttccttcaa
     1021 gctttgccac gccacggtat ttccccgata ccttgtgtgc aaattgcatc agatagttga
     1081 tagccttttg tttgtcgttc tggctgagtt cgtgcttacc acagaataca gccattccga
     1141 atccggcttg tgattgcgcc atccccatag cagccatcac atcagtaccg gaaagagagt
     1201 cagaagccgt ggcccgtggt gagtcgctca tcatcgggct ttttggcgaa tgaaatttag
     1261 ctacgctttc gagtctcatg cgccttctcc ctgtacctga atcaatgtga ggtttccgca
     1321 gaacactgcg ccagtatcga tatacatctg gttggcaaat ttgagtggtt tcactgct
//
LOCUS       DBFIFM010000412         1351 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.422, whole genome shotgun sequence.
ACCESSION   DBFIFM010000412 DBFIFM010000000
VERSION     DBFIFM010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.422"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..865)
                     /locus_tag="N7C74_004943"
     CDS             complement(<1..865)
                     /locus_tag="N7C74_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HGU2434618.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELHSQLTEIRGSAVGMAGAFAGAFASGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSAGDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAWVGGANEASG
                     VTKTLSGMLNGV"
     gene            complement(918..1127)
                     /locus_tag="N7C74_004944"
     CDS             complement(918..1127)
                     /locus_tag="N7C74_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HGU2434619.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSLAAMS"
     gene            complement(1223..>1351)
                     /locus_tag="N7C74_004945"
     CDS             complement(1223..>1351)
                     /locus_tag="N7C74_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HGU2434620.1"
                     /translation="DLQRVFTPDDREQVLAVYGPVHARLLRQALELIADAESARKK"
BASE COUNT          290 a          449 c          320 g          292 t
ORIGIN      
        1 caacaccgtt cagcatgccg gagagcgttt ttgtcacccc gctggcctca ttcgcaccgc
       61 ccacccaggc cataaaggcg ttttccacct tcgtgatact actggaaacc gtttccggca
      121 tggccgcata ttcatcacgt aatatcccca gctggctgat taacgcgggg accactttat
      181 ccgctgtcag ttttccgtca tccgccattg ccttcagatc tttacgggcc acgcccatac
      241 ccgcagccag tgcacgtacg atccggtcac cactttcatt gaccgaatta aattcctcac
      301 cgcgcaacac accctgtgcc agcgcctggc tgaactgggt gatcaccgaa cccgcctcag
      361 ccgtactggc accggagatt ttcagccccg tggaaatggc ctccgtcacc ttcagtacat
      421 caccggcact gtaaccatat tcacgcatcg aggcagccga acgggcaaac agggccgcat
      481 tatccgaaaa tgccgtgcct gtccgctggc tgatatccat cagcactttc tgtgatgacg
      541 aaaattcatc ggatgactgt gatgcctgtt tcagacgggc atttacggaa ctccactcat
      601 ccgccagtga aatcaggtgt ccggaggcaa aggcaccggc aaatgcgcca gccattccga
      661 cagcagaacc gcgaatttcc gtcaactggc tgtgcagttc tgccagggca cgtcgctgct
      721 cccgggctgc cgcagcggcc tgacgcccgc cattctgcag ggtccggtaa tattcactgc
      781 ccatacggga cgcccgctgg atctccgact ggaatgactg tgaatttgcc gaaattttga
      841 taatcagttc acgtaacgtc gccattcacc tttctccggg caaaaaaaaa cctgccacag
      901 caggttttca tcattattta tgacattgct gcaaggctca gcgcgtcttc cagcgccgca
      961 aacggatcca cctccggctt atcctcatcc tcgccccagc agagcatggc gtccttcagt
     1021 gcaacattca tcccctgtgc cccgaaaacc gctttcacga tctgtgcatt acggatatcc
     1081 ccgcgctcat cacccagcgg ggataccctg tcgaactcca tccacatcat cgcctcgctc
     1141 gcactcaggc tgtgccgcag ttcggataag gtgcgcccca gacggagcgc aagtcgcatc
     1201 agaaagcgaa tttccgggcg ggctactttt ttctggccga ctctgcatca gcgatcagtt
     1261 ccagtgcctg acgcagcaac cgggcatgta ccggaccata gacggccagc acctgctcac
     1321 ggtcgtccgg agtgaacacc cgttgcaggt c
//
LOCUS       DBFIFM010000413         1337 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.423, whole genome shotgun sequence.
ACCESSION   DBFIFM010000413 DBFIFM010000000
VERSION     DBFIFM010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.423"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..1236
                     /locus_tag="N7C74_004946"
     CDS             <1..1236
                     /locus_tag="N7C74_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU2434621.1"
                     /translation="SEKRIADIRQVETAARYLGSALYWIAASINIKPGHDYYFYIRSV
                     NTVGKSAFVEAVGRASDDAEGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTS
                     ITNVSNEITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGI
                     GAGIENTPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTI
                     TSGGNPPAFSLTPDGRLTAKNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGD
                     LVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHEKEHTDIY
                     SSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNNWYPTAS
                     ISDLLVVVMKKATAGITIS"
BASE COUNT          359 a          294 c          371 g          313 t
ORIGIN      
        1 tcggaaaagc ggattgcgga tatcaggcag gttgaaaccg cagcccgcta tcttggctcg
       61 gcgctgtact ggatagctgc cagtatcaat atcaaaccgg gccatgatta ttatttttat
      121 atccgcagtg tgaatactgt tggcaaatcg gcattcgtgg aggctgtcgg tcgggcgagc
      181 gatgatgcgg aaggttacct ggattttttc aaaggagaaa tcgggaaaac acatctggcc
      241 caggagctgt ggacgcagat tgataacggt cagcttgcgc cggacctggc tgaaatcagg
      301 acgtccatta cgaatgtcag caatgaaatc acgcagaccg tcaataaaaa actggaaaat
      361 cagagtgcgg caatccagca gatacagaaa gttcaggttg atacaaataa taacctgaac
      421 agcatgtggg ccgtgaaact gcagcagatg caggacggac gcctttatat tgcgggtatc
      481 ggtgccggta ttgagaatac gccagcagga atgcagagtc aggtgctgct ggcggcagac
      541 aggattgcga tgattaatcc tgcgaatggc aacacaaagc cgatgtttgt tggtcagggc
      601 gatcagatat ttatgaatga agtgttcctg aaatatctga cggctcccac cattaccagc
      661 ggcggtaatc ctccggcatt ttccctgaca ccggacgggc ggctgacggc gaaaaatgcc
      721 gatatcagcg gtaacgtgaa tgcgaactcc gggacgctca acaacgtcac gattaacgag
      781 aactgtcggg ttctgggaaa attgtccgcg aaccagattg aaggcgatct cgttaaaaca
      841 gtgggcaaag ctttcccccg ggactcccgt gcaccggagc ggtggccatc aggaaccatt
      901 accgtcaggg tttatgacga tcagccgttt gaccggcaga ttgttattcc ggcggtggca
      961 ttcagcggcg ctaaacatga gaaagagcat actgatattt actcctcatg ccgtctgata
     1021 gtgcggaaaa acggtgctga aatttataac cgtaccgcgc tggataatac gctgatttac
     1081 agtggtgtta ttgatatgcc tgccggtcac ggtcacatga cactggagtt ttcggtgtca
     1141 gcatggctgg taaataactg gtatcccaca gcaagtatca gcgatttgct ggttgtggtg
     1201 atgaagaaag ccactgcagg catcacgatt agctgaattt tataacccag atacgggcgc
     1261 cagaaatggc gcctttttta ttgcagaaaa gcgagaggta attatgcgta aattatgtgc
     1321 tgttattttg tccgcag
//
LOCUS       DBFIFM010000414         1328 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.424, whole genome shotgun sequence.
ACCESSION   DBFIFM010000414 DBFIFM010000000
VERSION     DBFIFM010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.424"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..262
                     /locus_tag="N7C74_004947"
     CDS             <1..262
                     /locus_tag="N7C74_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HGU2434622.1"
                     /translation="QATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQE
                     EERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            262..537
                     /locus_tag="N7C74_004948"
     CDS             262..537
                     /locus_tag="N7C74_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU2434623.1"
                     /translation="MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            632..838
                     /locus_tag="N7C74_004949"
     CDS             632..838
                     /locus_tag="N7C74_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434624.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSIAVAEMFHNAAWGVRTLW
                     FELVTKIDIDIHKKNRYASYDLDR"
     gene            complement(938..1153)
                     /locus_tag="N7C74_004950"
                     /pseudo
     CDS             complement(938..1153)
                     /locus_tag="N7C74_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1197..>1328)
                     /locus_tag="N7C74_004951"
     CDS             complement(1197..>1328)
                     /locus_tag="N7C74_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU2434625.1"
                     /translation="NRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          365 a          306 c          325 g          332 t
ORIGIN      
        1 acaagcaaca aaagtcatta ccgcgcatgt gccattacct atggctgata aagtcgacca
       61 gatggccgcc agactggaac gctcccgggg ttgggttatc aaacaggcgc tttctgcatg
      121 gcttgcccag gaggaggagc gtaatcgcct gacgctggaa gccctggacg atgtgacatc
      181 cggacaggtt attgaccatc aggctgtaca ggcctgggcg gacagcctca gtactgacaa
      241 tccgttaccg gtgccacgct gatggaactg aagtggagca gtaaagcgct ttctgatttg
      301 gcgcggttat atgattttct ggtgctagcc agtaaacctg ctgccgccag aacggtacag
      361 tccctgacac aggcaccggt cattctgtta actcatccac gtatggggga acagttattt
      421 cagtttgaac ccagggaggt cagacggatt tttgctggcg agtacgaaat ccgttacgaa
      481 cttaatggcc agactattta tgtattgcgc ctgtggcaca cacgagaaaa caggtagatt
      541 gtgtgtgggg acaacttaac atagcctgca gatattaatt ctggaacatt attatcaggc
      601 cagatattta aataatcttg taggagataa aatgaattac gatgaaataa cgaaaattac
      661 cgctgaacgt attagcgact acatgactga agcagttaat acagactcta tagccgttgc
      721 ggaaatgttt cataacgcag catggggagt gcgaacattg tggtttgaac tcgtcacaaa
      781 aatagatata gatatacata aaaagaacag atatgcgagt tatgatcttg atcgatagga
      841 atttaaatcc caaaaagatt aaaaaaacac ccaaaaacgg atgtttattc aacactgcct
      901 gcgtgccatg agtgcaccgc cgtcaattaa atggatctat ttcatcctga ccagcatagt
      961 gctccagacg gctcatcggc accggcggtt ttttcaggta agagccgaag tacaccgcca
     1021 catgtgtggc gttatccatc acccgggata cgttgacatt ccagccccgc cggtaatgac
     1081 tgtccaggaa gcgattccag tcccgtttac tgcttccttc tgctgccagc gcatccggta
     1141 tcaccaggtc cggtgaggtg tactggcaat agcggacact accatttgtt ctttttttaa
     1201 gcagccatct gatgatattt ttccctgaag gctgccgggg agatattccc cagacgagag
     1261 tgacgacgct gacgattgta gaaaatctca atgtattccc gtattactga gatggcttca
     1321 tcccggtt
//
LOCUS       DBFIFM010000415         1319 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.425, whole genome shotgun sequence.
ACCESSION   DBFIFM010000415 DBFIFM010000000
VERSION     DBFIFM010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.425"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            93..656
                     /locus_tag="N7C74_004952"
     CDS             93..656
                     /locus_tag="N7C74_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU2434626.1"
                     /translation="MLTTQKRKFALALMSGKNKTASALAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEPEDKPRRREAAAIPQPDETNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            653..>1319
                     /locus_tag="N7C74_004953"
     CDS             653..>1319
                     /locus_tag="N7C74_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU2434627.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQ"
BASE COUNT          313 a          340 c          413 g          253 t
ORIGIN      
        1 ccaggcagca gttaacattt gcggcatgag acgccgggaa aaatgggctg aaccataccc
       61 ggctgagtgc gttctggacc cgggaggagg ctgtgctgac aacgcaaaaa cgaaaatttg
      121 cgctggcgct catgtccggg aaaaacaaaa cagcgtcagc ccttgccgcc ggttattcgg
      181 cgaagacagc cagggttaaa ggctcgcagc tggcaaaaga tccggaggtg cttgcgttta
      241 tagcccgtaa acagtgcgag acggtggagg tggatgaggt tcctgtttat cggcagaaaa
      301 aatcagagcc ggaggataaa ccccgtcgcc gtgaggcggc tgcaatacca cagccggacg
      361 aaacaaatcc ggagatgcca ccgcccgtgg tgatatctcc tggtattgag tatatggagg
      421 acggtcttcc cgatccggtg aaagcgatgg ggcgtcttct ggtggagaac attaataccg
      481 accccaggct ggcgctggat gcggcttata agctggcgca gttcacgcac cacaaaaaag
      541 gggatgccgg taaaaaatcg gcaaaaggtg acgcggcgaa aaaagcggct aaccgttttg
      601 cggtgccacc accaccccgc ctggtggtga ataatgataa tgagggcaac ggatgatacc
      661 tgtgtggagc acggcctgcc cggactgggc agagcgcctg aaaaaggggc tgtcgattat
      721 tccggctccg atttatccgg agcaggccgc acatgccctg gcgattttta aacaactgcg
      781 gattgtggat gcaccgggca gcccgacgtt cggggagtcc tgcgcacagt gggtgtttga
      841 cctggtggcg gccctgtttg gctcctacga tgcgcagacc ggtgtacgcc atatcaagga
      901 agtttttatc cttatcccca agaaaaacag caagtccacg ctggctgccg ggatcatgat
      961 gacggcgctg ttactgaact ggcggcaggc ggcgggctac accattctgg ccccgaccgt
     1021 ggaggtggcg gctaacgcct tcaaccctgc cagggatatg gtacgacggg acgatgatct
     1081 ggatgacctc tgtcaggtgc agacacatat ccggaccatc acccacaggg tgacggacac
     1141 caccctgaag gtggtggctg ccgatccgaa tacggtatcc ggtatcaagt ccgtggggac
     1201 gctgattgat gaactgtggt tatttggcaa gcagtacaaa gcggaggaca tgttacgtga
     1261 agccataggc ggccttgcct cccgcccgga agggtttgtg gtgtatacga ccacccagt
//
LOCUS       DBFIFM010000416         1313 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.426, whole genome shotgun sequence.
ACCESSION   DBFIFM010000416 DBFIFM010000000
VERSION     DBFIFM010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.426"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            106..210
                     /locus_tag="N7C74_004954"
                     /pseudo
     CDS             106..210
                     /locus_tag="N7C74_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(330..>1313)
                     /gene="nleC"
                     /locus_tag="N7C74_004955"
     CDS             complement(330..>1313)
                     /gene="nleC"
                     /locus_tag="N7C74_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HGU2434628.1"
                     /translation="PSLQSNFNFSAPAGYSAPIAPNRAENAYADYVLDIGKRIPLSAA
                     DLSNVYESVIRAVHDSRSRLIDQHTVDMIGNTVLDALSRSQTFRDAVSYGIHNEKVHI
                     GCIKYRNEYELNEESSVKIDDIQSLTCNELYEYDVGQEPIFPICEAGENDNEEPYVSF
                     SVAPDTDSYEMPSWQEGLIHEIIHHVTGSSDPSGDSNIELGPTEILARRVAQELGWSV
                     PDFKGYAEPEREAHLRLRNLNALRQAAMRHEENERAFFERLGTISDRYEASPDFTEYS
                     AVSNIGYGFIQQHDFPGLAINDNLQDANQIQLYHGAPYIFTFGDVDKHNQQ"
BASE COUNT          349 a          292 c          277 g          395 t
ORIGIN      
        1 caaaatattg ttgctgcaat ggacctgtac aatagtgaat cacggtactt ttctgtgcac
       61 aacgtactgc agtttcttta cgatttatag ctgtactgtt gaattatgta caatttgaat
      121 ccatgcatga cgtgtgatgc ctattgtgca tttttccgag cctcttttta ctgggccgaa
      181 gccgatgatg ctgatagtct cgttccggcc atgttcactt actacagtaa aattagggga
      241 attgccttta catacaatgt tgcacgatta cccatcatca actccgttaa aatgtatgaa
      301 tagtaacctt atgtcactgc aaagacgaat cattgctgat tgtgtttgtc cacatcccca
      361 aatgtaaaaa tataaggggc gccatgatac agttggatct gatttgcatc ctgtaaatta
      421 tcgttgatag ccaatccagg aaaatcatgt tgctggataa atccgtatcc tatgttagac
      481 acagcggaat actctgtgaa atcaggactc gcctcatatc ggtcactgat cgtacccagc
      541 ctttcgaaga aagccctctc attctcttca tgcctcatgg cagcctgtcg aagggcattc
      601 aggttacgta gcctaagatg agcttcacgt tctggctctg catatccttt gaagtcggga
      661 acactccatc ccagttcttg agcgacacga cgtgcgagaa tctcggtggg tcctaactct
      721 atattactat ctccagatgg atcgctagat ccagtaacat gatgaataat ctcgtgaatc
      781 agtccttcct gccacgatgg catctcataa gagtcagtat ctggcgcaac actaaaactg
      841 acataaggct cttcgttatc gttttctcct gcttcgcaaa tggggaaaat tggctcttgc
      901 ccgacatcat attcatataa ttcgttacag gttagtgatt gaatatcatc aattttgaca
      961 gaagattctt cgttaagctc gtattcgttt ctgtatttaa tgcaaccaat gtgtaccttc
     1021 tcattatgaa tgccatagct tacggcatca cgaaatgtct gtgatcggct caaagcatca
     1081 agtacagtgt tgccgatcat atcgactgta tgctgatcga taagcctgct acggctgtca
     1141 tggacggcgc gtattacact ttcgtatacg ttgcttaaat ctgctgcgga aagtggtatt
     1201 cgcttaccta tatccaaaac gtaatccgca taggcatttt cagcacgatt aggagcaatg
     1261 ggagcagagt atcctgccgg ggcggaaaag ttgaagttgg actgtaatga ggg
//
LOCUS       DBFIFM010000417         1303 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.427, whole genome shotgun sequence.
ACCESSION   DBFIFM010000417 DBFIFM010000000
VERSION     DBFIFM010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.427"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..228)
                     /locus_tag="N7C74_004956"
     CDS             complement(<1..228)
                     /locus_tag="N7C74_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HGU2434629.1"
                     /translation="MNKQRIFIAGHQGMVGSAITRRLKQRDDVELVLRTRDELNLLDS
                     SAVLDFFSSQKIDQVYLAAAKVGGILANSSYP"
     gene            complement(231..>1303)
                     /gene="gmd"
                     /locus_tag="N7C74_004957"
     CDS             complement(231..>1303)
                     /gene="gmd"
                     /locus_tag="N7C74_004957"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HGU2434630.1"
                     /translation="YLAEFLLDKGYEVHGIKRRASSFNTERIDHIYQDPHGSNPNFHL
                     HYGDLTDSSNLTRILKEVQPDEVYNLAAMSHVAVSFESPEYTADVDAIGTLRLLEAIR
                     FLGLENKTRFYQASTSELYGLVQEIPQKESTPFYPRSPYAVAKLYAYWITVNYRESYG
                     IYACNGILFNHESPRRGETFVTRKITRGLANIAQGLESCLYLGNMDSLRDWGHAKDYV
                     RMQWLMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKMSFVGKGIEEKGIVDSVEG
                     QDAPGVKPGDVIVAVDPRYFRPAEVDTLLGDPSKANLKLGWRPEITLAEMISEMVAKD
                     LEAAKKHSLLKSHGFSVSLALE"
BASE COUNT          405 a          282 c          233 g          383 t
ORIGIN      
        1 aggataagaa ctgttagcta aaatacctcc gacttttgct gctgccaaat aaacctggtc
       61 gattttctgt gaagaaaaaa aatccaaaac agcgctacta tccaacaagt tcaattcatc
      121 ccgagtacgt aaaaccaact caacatcatc acgttgtttg aggcgtcggg taatagctga
      181 tccaaccatt ccttggtgac cagcaataaa aatacgttgc ttattcatca tcattccaga
      241 gctaagctta cagaaaaacc atgcgatttt aaaagagaat gttttttagc ggcttcaaga
      301 tctttggcaa ccatttcaga aatcatttca gcaagagtaa tttctggtct ccaaccaagt
      361 ttgagattag ctttgctcgg atctccaagc aaagtatcaa cttcagctgg tcggaaataa
      421 cgaggatcaa cagcaacaat gacatcacct ggtttcacac ctggagcatc ctgtccttca
      481 accgaatcta caatgccttt ttcttcgatt cctttaccaa caaagctcat cttaatacca
      541 agttgtgctg ctgccatttc gacaaactga cggactgagt attggactcc tgttgcaatc
      601 acaaaatctt cgggttgctc ctgttgtaac atcaaccatt gcattctaac ataatctttt
      661 gcatgtcccc aatctcgtaa cgaatccata ttccctaaat acaaacatga ttccaagcct
      721 tgtgcaatat ttgcaagtcc tcgagtaatt ttccttgtta caaacgtttc tccacggcgt
      781 ggagattcat gattgaacaa tataccatta catgcataaa taccatatga ctctcgataa
      841 tttaccgtga tccaatatgc gtaaagtttt gcaactgcat aaggggaacg aggataaaaa
      901 ggggtggatt ctttttgagg gatttcctga acaagtccat ataattctga ggttgaagct
      961 tgatagaaac gcgttttgtt ttccaatcct aaaaagcgaa ttgcttccag taaacgtaat
     1021 gtaccaattg catcgacatc ggctgtatat tctggagact caaaagaaac tgctacgtga
     1081 ctcatagcag ctaaattata tacttcatct ggctgtacct cctttagaat tctagtgagg
     1141 ttagatgaat cagtcagatc tccatagtgc aagtgaaaat ttgggttaga accatgtgga
     1201 tcttgataaa tatggtctat gcgttctgta ttaaaagatg aggctcggcg tttgatacca
     1261 tgaacttcat accctttatc aagcaaaaac tcagctagat aag
//
LOCUS       DBFIFM010000418         1277 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.428, whole genome shotgun sequence.
ACCESSION   DBFIFM010000418 DBFIFM010000000
VERSION     DBFIFM010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.428"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            6..81
                     /locus_tag="N7C74_004958"
     tRNA            6..81
                     /locus_tag="N7C74_004958"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="N7C74_004959"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="N7C74_004959"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(467..1225)
                     /gene="yhdZ"
                     /locus_tag="N7C74_004960"
     CDS             complement(467..1225)
                     /gene="yhdZ"
                     /locus_tag="N7C74_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGU2434631.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
BASE COUNT          324 a          334 c          304 g          315 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggactccc catgcgagag tagggaactg ccagacatca
      241 aataaaacaa aaggctcagt cggaagactg ggccttttgt tttatctgtt gtttgtcggt
      301 gaacactctc ccaaccagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
      361 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc agacatcaaa tcaagcgaaa
      421 ggccatccga aaggatggcc tttatgcttt tcgaactaac attcaattaa tggattacct
      481 gcgataaaaa tgccctcgta cgctctgact taggatgcgc aaaaaactca tccggtgcag
      541 cttgctccac tatttcgcca cgatccataa aaatcacccg gtcagcgacg gttcgcgcaa
      601 accccatctc atgggttaca cacaacattg tcatacccga ttgcgccagc ccaatcatcg
      661 tatccagcac ctctttcacc atctccggat cgagcgccga tgtgggctca tcaaacaaca
      721 taattttcgg cttcatacac agcgagcgcg caatggcaac gcgttgctgc tgaccacctg
      781 aaatctgtcc gggaaactta tgcgcatgtt cggcaattct cacccgctca aggtaatgca
      841 tcgccagagc ttcagcctct ttctttggca tcttgcgtac ccaaatcggt gccagggtac
      901 agttctgtag aacggtcaga tgagggaaga gattgaaatg ctgaaagacc attcccactt
      961 cctgcctgac gcgctcaata ttgcggatat cttcattaag ttcgatgcca tctaccacga
     1021 tccgtccctg ttgatgttct tccagatgat taatacaacg aatggttgtc gatttaccgg
     1081 aacctgaagg gccacacaga acgatccgtt ctcccggttg cacggttaaa tttatatttt
     1141 tcaaaacatg gaattgtcca taccatttat tgacgttttc cagcgtaatc atcgcgttag
     1201 caggttgcag taaaatttgg ctcatagtgt cctcaatgcg gtgtacgccc ggtgttaaaa
     1261 cgtttttcca gatgctg
//
LOCUS       DBFIFM010000419         1276 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.429, whole genome shotgun sequence.
ACCESSION   DBFIFM010000419 DBFIFM010000000
VERSION     DBFIFM010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.429"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..269
                     /locus_tag="N7C74_004961"
     CDS             <1..269
                     /locus_tag="N7C74_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Replication protein O"
                     /protein_id="HGU2434632.1"
                     /translation="ARKPNFAGWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNV
                     LSPAKLRDKWTQLEINRNKQQAGVTASKPKLDLTNTDWIYGVDL"
     gene            266..967
                     /locus_tag="N7C74_004962"
     CDS             266..967
                     /locus_tag="N7C74_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU2434633.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRVQALAK
                     IAEIKAKFGLKGASV"
     gene            964..1254
                     /locus_tag="N7C74_004963"
     CDS             964..1254
                     /locus_tag="N7C74_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HGU2434634.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLAVYGVKR"
BASE COUNT          348 a          313 c          364 g          251 t
ORIGIN      
        1 cagccagaaa accgaatttt gctgggtggg ctaacgatat ccgcctgatg cgtgaacgtg
       61 acggacgtaa ccaccgcgat atgtgtgtgc ttttccgctg ggcctgccag gacaacttct
      121 ggtccggtaa cgtgctgagt ccggccaaac tccgcgacaa gtggacccag ctcgaaatca
      181 accgtaacaa gcaacaggca ggcgtgacag ccagcaaacc aaaactcgac ctgacaaaca
      241 ctgactggat ttacggggtg gatttatgaa aaacatcgcc gcacagatgg ttaactttga
      301 ccgtgagcag atgcgtcgaa tcgccaacaa catgccggaa cagtacgacg aaaagccgca
      361 ggtacaacag gtagcgcaga tcatcaacgg tgtgttcagc cagttactgg caactttccc
      421 ggcgagtctg gctaaccggg accagaacga actgaatgaa atccgccgcc agtgggttct
      481 ggctttccgg gaaaacggga tcaccacaat ggaacaggtt aacgcaggaa tgcgcgtagc
      541 ccgtcggcag aatcgaccat ttctgccatc acccgggcag tttgttgcat ggtgccggga
      601 agaagcatcc gttatcgccg gactgccaaa cgtcagcgag ctggttgata tggtttacga
      661 gtattgccgg aagcgaggcc tgtatccgga tgcggagtct tatccgtgga aatcgaacgc
      721 gcactactgg ctggttacca acctgtacca gaacatgcgg gccaatgcgc tgactgacgc
      781 ggaattacga cgcaaggctg ccgatgaact gacctgtatg acagcgcgaa ttaaccgtgg
      841 tgagacgata cctgaaccag taaaacaact tcctgtcatg ggcggcagac ctctaaatcg
      901 tgttcaggcg ctggcgaaga tcgcagaaat taaagctaag ttcggactga aaggagcaag
      961 tgtatgacgg gcaaagaggc aattattcat tacctgggga cgcataatag cttctgtgcg
     1021 ccggacgttg ccgcgctaac aggcgcaaca gtaaccagca taaatcaggc cgcagctaaa
     1081 atggcacggg caggtcttct ggttatcgaa ggtaaggtct ggcgaacggt gtattaccgg
     1141 tttgctacca gggaagaacg ggaaggaaag gtgagcacga acctgatttt taaggagtgt
     1201 cgccagagtg ccgcgatgaa acgggtattg gcggtatatg gagttaaaag atgaccatct
     1261 acatcactga gctaat
//
LOCUS       DBFIFM010000420         1274 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.430, whole genome shotgun sequence.
ACCESSION   DBFIFM010000420 DBFIFM010000000
VERSION     DBFIFM010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.430"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..73
                     /locus_tag="N7C74_004964"
     CDS             <1..73
                     /locus_tag="N7C74_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="activates the production of the major fimbrae
                     protein FimA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbriae biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HGU2434635.1"
                     /translation="IYGKLGLHSIVELIDYAKLYELI"
     gene            complement(76..591)
                     /gene="sfmF"
                     /locus_tag="N7C74_004965"
     CDS             complement(76..591)
                     /gene="sfmF"
                     /locus_tag="N7C74_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HGU2434636.1"
                     /translation="MRRVFFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALLPFSLRLEGCPPGSVAILFTGTPASDANLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(602..>1274)
                     /gene="fimH"
                     /locus_tag="N7C74_004966"
     CDS             complement(602..>1274)
                     /gene="fimH"
                     /locus_tag="N7C74_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HGU2434637.1"
                     /translation="SVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPF
                     INMVEIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDI
                     GASLFSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDL
                     GFIVADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYL
                     RVDYQ"
BASE COUNT          358 a          329 c          279 g          308 t
ORIGIN      
        1 tatttatggc aagctaggtt tgcattcaat tgtagagctt atcgactacg ccaaattata
       61 cgaattaata taatattaat tataattgat cataaatatc gcatccgctt tcgccacacc
      121 tggccgaaca ccgctggcta acgctcgata attggcaaaa aagtttagtg tgacattgcc
      181 atttgcatct acttcctcag tcgggctcgc ctcccccagt gcgagccggg agcgatcgct
      241 attacgtaat tcgatggcga cggtttgtgc cattgcggga tcatccagag ccagcaggtt
      301 ggcatcagat gccggcgttc ccgtaaataa aatcgcaact gaacccggag gacatccctc
      361 cagccgcagg ctaaaaggga ggagtgccgt ggtatcgcca gcgttcagta attgtgtcgt
      421 aggccatctg cctaaatcta ccgtcttatc aatatccgct gtgtttacgg tacaggagaa
      481 atcaacaacg ttaccgtgca aattgatatt aatcgttcct aaagggtcag ctgcccatcc
      541 actggaactc cacagtaacc cgcagaaaca gctaaagaat actcttctca ttctccgtac
      601 ctcattgata atcgacgcgt aaatacccca gcgcgctaaa cggcccttcg gtcggttttt
      661 gaccggtaat actgataggc caggcgcgaa gtgtgacatt ggctgccgca gctgcatcca
      721 gacggaaagg aataacgcta ttgagatcgt taggcgtgat cggcgtatcg ttctgatcgg
      781 cgacaataaa acctaaatcc tgattgtccg acaccatcgc ctgaccagaa acggcactgg
      841 cttccagacg cattgttaaa taagcctgcg cagcaacatt cgtacatttg accgcaatgc
      901 tcttggtttg cggcatgaca ccagcaggcc gattacccgg ccctgccgca ctaaataacg
      961 atgcgccgat atcaccaaaa tcaaattcaa caatctgccc ggcatttaat tcgcagtttt
     1021 gcggtacttc aacccggcca ccaaaactaa tggtataaac aggtgtaacc aacggatcgt
     1081 aaggcgttga tgtcacatac acggtaaaca tcacctgtct ggggatctcc accatgttaa
     1141 taaagggccg aataaccttt aaacgaaata tcaaacgaga atccatcacc ccgaacggaa
     1201 ggttttgcga aacgttagga tctgttccca tacgaatata attttgcggg ggaaattgaa
     1261 tatccatcac acta
//
LOCUS       DBFIFM010000421         1264 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.431, whole genome shotgun sequence.
ACCESSION   DBFIFM010000421 DBFIFM010000000
VERSION     DBFIFM010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.431"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(174..1262)
                     /gene="ompC"
                     /locus_tag="N7C74_004967"
     CDS             complement(174..1262)
                     /gene="ompC"
                     /locus_tag="N7C74_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HGU2434638.1"
                     /translation="MVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYFTDDK
                     RDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRDNKTRLAYAGLKIS
                     EFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGVATYRNYDFFGLI
                     EGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKSDRTN
                     AQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNKAQSF
                     EVVAQYQFDFGLRPSLAYLKSKGIDLGRYGDQDMIEYIDVGATYFFNKNMSTYVDYKI
                     NLIDESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT          362 a          281 c          270 g          351 t
ORIGIN      
        1 gtcataatca ctcatcaata aatattatag taagatgtcc ccgggggtat ggaataatat
       61 taattgaggt gaatgaccag aaatatcaaa aattgctcat ttttacttaa ggagtcatac
      121 agcagcactt aaatcaaaaa ccagcccaga gaagggctgg tcaggagagg atgttagaac
      181 tgataggtaa tgcccgtagc gacgatgtta tcggtgcgaa tatctacggc acgggtaaag
      241 tcgctttcat caatcaggtt gattttataa tcaacatagg tcgacatatt tttgttgaag
      301 aaatacgtcg caccaacgtc gatatactca atcatgtcct gatcgccgta gcggcccaga
      361 tctatgcctt tcgatttcag gtaagcgagt gaggggcgca agccgaagtc gaattgatat
      421 tgcgccacca cttcaaaact ttgtgctttg ttagaaatat aaccatcgcc ataggtggtc
      481 atgtttaatg tttcagcgta attagctgca aagtagatgt tgttggcatc atattttata
      541 cctgtagccc acagttctgc attcttaccg gaggcattga gaggattagc agcggctctt
      601 tcctgcgcat ttgtccgatc ggatttggta tacaccgctc cgataccaaa gccatcataa
      661 acataagttg aggagatacc gaaaccgtca ccgttggcac gcgtgtagtc agcaccataa
      721 agatgactgt tgtcagtacg ttcatttttg ccttgatatt gcgcggcaaa gttcagccct
      781 tcaattaagc caaagaaatc gtagttgcga taggttgcta caccggtggt acggtatgtc
      841 atgaagacat ctttctgact ccaggtatcg ccaccaaatt ctggcaacat atcagtccat
      901 gaaccaatgt catagccgac accgtagtta cggccatagt ccagagagcc aaattcacta
      961 atttttaaac cagcataggc cagacgggtc ttgttgtcgc gcgagccgtt ggcttcatcg
     1021 ttatagcctt taaaatcata ttcccagtga ccaaaaccaa tcatttgatc attgatttgc
     1081 gtttctcctt taaagccgag acgggcataa gttttatcac catcgtcacg cttatcatca
     1141 gtaaaataac gtagagcggt aaccttgcca taaaggtcga gtttattacc atccttgtta
     1201 tagacttcgg cagcgtgtgc tacgttcgca gctaacaaac cagttacaac caccgcaact
     1261 attt
//
LOCUS       DBFIFM010000422         1257 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.432, whole genome shotgun sequence.
ACCESSION   DBFIFM010000422 DBFIFM010000000
VERSION     DBFIFM010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.432"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(16..588)
                     /locus_tag="N7C74_004968"
     CDS             complement(16..588)
                     /locus_tag="N7C74_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HGU2434639.1"
                     /translation="MPGLISYVSSASFVNEMMELRQQVMEGQIGGFLLGGERVRVSYM
                     PDTGRFLAESEGQGRVYAELLNIAFNDGVNVLRNRILSALPGMGGRNSLQEKISECAF
                     TVDIEKLQCPGDALQCPITLEQPEKGVFVKNSDGSDVCTLFDAAAFSRLTGEDLPHPL
                     TREPITASIIVKHEECIYDDTRGNFVIKGN"
     gene            complement(621..698)
                     /locus_tag="N7C74_004969"
                     /pseudo
     CDS             complement(621..698)
                     /locus_tag="N7C74_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            complement(700..969)
                     /locus_tag="N7C74_004970"
     CDS             complement(700..969)
                     /locus_tag="N7C74_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU2434640.1"
                     /translation="MNILKKIMQRLCGCGKHDDCEHGQSLTVQLRLGPADILESDENG
                     IIPEQVRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            complement(971..>1257)
                     /locus_tag="N7C74_004971"
     CDS             complement(971..>1257)
                     /locus_tag="N7C74_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU2434641.1"
                     /translation="GPVGATGPQGPKGDPGETQIRFRLGPGNIIETNSNGWFPDTDGA
                     LITGLTFLAPKDATRVQVFFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
BASE COUNT          315 a          367 c          234 g          341 t
ORIGIN      
        1 agggtaatgt tcatttcaat taccctttat aacgaagttt cctctggtat cgtcataaat
       61 gcattcttca tgttttacaa ttattgatgc cgttattggt tcccgggtca gtgggtgggg
      121 taagtcttca ccagtcaaac gagaaaatgc agcggcatca aataaagtac atacatctga
      181 accatctgaa ttcttcacaa aaacaccttt ttcaggctgc tccagtgtaa ttggacattg
      241 aagcgcatca ccaggacact gaagtttttc aatatcgaca gtaaaggcac actccgatat
      301 tttttcctgc aaagagtttc gccctcccat tccagggagc gcacttaata tcctgtttct
      361 gagcacatta actccatcat taaaagcaat attcaataat tctgcataaa cccgtccctg
      421 cccttcactt tctgctaaaa aacggcctgt atctggcata taagaaactc taaccctctc
      481 ccctcccagg agaaatccac caatctgccc ctccattacc tgctgacgca gctccatcat
      541 ctcattcacg aatgaagccg atgacacata tgatattaat ccaggcatta ccatgctcct
      601 tcatacggat aaatattccc tcaagtcttc attaacaaac atccaccgga catgacaaca
      661 aaaaccggag ccggactccg gttttgtgaa gctgtcgggt tacttcatcc cgccaatatt
      721 ttcccacgtc ccgtcagcac gcaggatttg cagcggtctt accacacact gtatctgctt
      781 tttatccgca tccagtatca ccacctgcgt gattaccctg acctgctccg ggataatgcc
      841 attctcatct gactccagga tgtctgccgg ccccagtcgc agttgtactg taagcgactg
      901 cccgtgttca cagtcatcat gctttccgca accgcacaga cgctgcataa ttttttttaa
      961 tatgttcatg tcattctcct gttctgcctg tatcactgcc cacttcatcc agccccttaa
     1021 catcctgcca cggcccgtca ccaaacctga cctgcaaatg ctgaaaaaaa acctgaaccc
     1081 gtgtggcatc tttgggggca agaaaggtca gtccggtgat gagtgcgcca tctgtatccg
     1141 ggaaccagcc attgctgttt gtctcaataa tgtttcccgg ccccagacga aaacggattt
     1201 gtgtctcccc cgggtcgccc ttcggtccct gaggtccggt tgcccccacc gggccag
//
LOCUS       DBFIFM010000423         1252 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.433, whole genome shotgun sequence.
ACCESSION   DBFIFM010000423 DBFIFM010000000
VERSION     DBFIFM010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.433"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            83..430
                     /gene="tnpB"
                     /locus_tag="N7C74_004972"
     CDS             83..430
                     /gene="tnpB"
                     /locus_tag="N7C74_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU2434642.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            480..>1252
                     /locus_tag="N7C74_004973"
     CDS             480..>1252
                     /locus_tag="N7C74_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HGU2434643.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELRRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPG"
BASE COUNT          268 a          363 c          372 g          249 t
ORIGIN      
        1 taacgttccg gcacgggacg ctccgcttca atggcaatgt cagcgaaaag ctcctgactc
       61 tgctgataca ggaactgaag cgatgatccc gttaccttcc gggaccaaaa tttggctggt
      121 tgccggtatc accgatatga gaaatggctt caacggcctg gctgcgaaag tacaaacggc
      181 gctgaaagac gatcccatgt ccggccatgt tttcattttc cggggccgca gcggcagtca
      241 ggttaaactg ctgtggtcca ccggtgacgg actgtgcctc ctgaccaaac ggctggagcg
      301 tgggcgcttc gcctggccgt cagcccgtga tggcaaagtg ttccttacgc aggcgcagct
      361 ggcgatgctg ctggaaggta tcgactggcg acagcctaag cggctgctga cctccctgac
      421 catgctgtaa atctctttat cctggttgtc acagaataag cccggtaaaa tacgggctta
      481 tgaacgacat ctcttctgac gacatcttcc tgctgaaaca gcgcctggcc gaacaggaag
      541 cgctgatcca cgccctgcag gaaaagctga gcaaccggga gcgcgaaata gaccatctgc
      601 aggcgcagct ggataaactc cgccggatga acttcggcag tcgttccgaa aaagtctccc
      661 gccgtatcgc acaaatggaa gccgatctga accggcttca gaaagagagc gatacgctga
      721 ctggtagggt gtatgacccg gcagtacagc gtccgttgcg tcagacccgc acccgtaagc
      781 cgttccctga atcactaccc cgtgacgaaa agcgactgtt gcctgcggcg ccgtgctgcc
      841 cgaactgcgg cggttcactg agctatctgg gcgaggatac cgccgaacag ctggagttga
      901 tgcgtagcgc cttccgggtt atccggacgg tacgggaaaa acatgcctgt actcagtgcg
      961 atgccatcgt gcaggcacct gcaccttcgc ggcccatcga gcggggtatc gccggaccgg
     1021 ggctgctggc ccgcgtgctg acctcgaagt atgcagagca caccccgctg tatcgccagt
     1081 cagaaatata cggccggcaa ggtgtggagc tgaggcgttc actgctgtcg ggctgggtgg
     1141 atgcatgctg ccggctgctg tctccgctgg aagaggcgct tcatggctat gtcatgactg
     1201 acggcaaact ccatgccgat gataccccgg tccaggtact gctgccgggt aa
//
LOCUS       DBFIFM010000424         1250 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.434, whole genome shotgun sequence.
ACCESSION   DBFIFM010000424 DBFIFM010000000
VERSION     DBFIFM010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.434"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(1..1248)
                     /locus_tag="N7C74_004974"
                     /pseudo
     CDS             complement(1..1248)
                     /locus_tag="N7C74_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225633.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
BASE COUNT          360 a          269 c          230 g          391 t
ORIGIN      
        1 aaattttaaa atcagatttt ttaaatgctt cctcatattc attcagagaa atttgttttt
       61 cccggatatt tttttcataa taagaaagct gaagagaagt aaatagcgcc agccttctct
      121 ccggaatcat caatggtttg actttcagta tcatgaagtt taacaacaaa tcaattctgt
      181 ttctgaatcc aatccgcctg tcagaaaggt aatgaatata agccatcagg tcggttgttt
      241 tttgtgagga aaatcggtat ttgtgagaaa aaagctcctc cggtcgaaga ttctgactct
      301 gccattgctg caggtattta tactcaatat tcagctcatt aatttctgct tttaacgaag
      361 cgacctggtt aatggcattc aggtgttttt tcacttgttg aagtacatca tcaatcgcat
      421 ttgcagacgg tgagtcaggt aagacttcct cagaacgcga aatcgacgtt gaaaaaaact
      481 gttgcctgtt ctccgtactc cccagtcgtg ccatcacata tcccagttgc tgtttgtcca
      541 tcttttcata aacattactg acagctgagt tattattgga caaaattgcc actgttttat
      601 tctgaattaa tatattagca acaatattga gtatagtctg tgttttacct gttccaggtg
      661 gtccttcaat aactgaaatc tgtgaggaaa atgcccgttc gacagccagc aactgacttt
      721 cattaagacc aaacggaaaa atcaaacccc atggcgaatc acgtttttac tctgtcctga
      781 acagtatgca tgcaaggcgg tatccggatg aggaactatt ttctttatct gcctaaggat
      841 attctcatcg atatttctgt tattaccacg ggcataaaaa atacgttcat tagccaccct
      901 acacaagtaa ttaaacacgg catgctctgt aaatgctgaa cactgttgaa agtcaaggcc
      961 ggtcgttttc atgatgtagg gtcgttcacc ctcgcggtaa taaacagcag catatttatc
     1021 accatatata ataaccagtt cagcccggga tgttacagcg ttccctttcc tttcgaggaa
     1081 aacatttcgc aaatttacag ttggcgttat ctgacataca gaaagcggac gagtatattt
     1141 tctcccgctg cgaaaatgac aggtcagcat aacctctctg tcgtcatttc cagcccacag
     1201 cgtccagtcg gtaattttat gagtcttatt ttcaccgtca acacaaatcg
//
LOCUS       DBFIFM010000425         1249 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.435, whole genome shotgun sequence.
ACCESSION   DBFIFM010000425 DBFIFM010000000
VERSION     DBFIFM010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.435"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..>1249
                     /locus_tag="N7C74_004975"
     CDS             <1..>1249
                     /locus_tag="N7C74_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU2434644.1"
                     /translation="GNVSGVTSREDYGRETQREYRLDRNGQVTAVTASGTGLGYGEGD
                     ESYGYDSCGYLKAQSAGGHRISEETDQYAGGHRLKQAGNTQYDYDAAGRMVSRTRHRD
                     GYRPETERFRWDSRDQLTGYCSAQGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDS
                     IAEIREYRDDELYSVRHLVFNGFELISQQCSRVRQPHPSVAPQWVTRTNHAVNDLTGR
                     PLMLFNSEGKTVWRPGQTSLWGLALSLPADTDYPDPRGERDAEADPGLLYAGQWQDAE
                     SGLCYNRFRYYEPETGMYLVSDPLGLQGGEQTYRYVPNPCGWVDPLGLAASSKISSLM
                     DYIGDGRRVSGHTGFLDGVRLSRSQINNIAKEMEKLGIKVIRKADKYLPPNARAAFDY
                     GLRNIYLRKNATLYEVYHEVIHAK"
BASE COUNT          307 a          302 c          408 g          232 t
ORIGIN      
        1 ggtaacgtat caggcgtcac cagccgggaa gattacggac gggaaacaca gcgggagtac
       61 cggcttgacc ggaacggcca ggtcacggcg gtgacagcct caggcaccgg gctgggctat
      121 ggcgaaggcg atgagtccta tggctatgac agctgtggct acctgaaggc gcagtctgcg
      181 ggcgggcacc ggataagtga agagactgac cagtatgccg gaggccaccg gctgaaacag
      241 gccggaaaca cgcagtatga ctatgacgcc gcaggccgga tggtcagccg caccagacac
      301 cgtgacggct atcgcccgga aacagagcgg ttccggtggg acagccggga ccagctgacc
      361 gggtattgca gcgcacaggg tgagcagtgg gaataccgcc acgacgccag cggcagacga
      421 acagagaaac gctgcgaccg gaagaaaatc cgcttcacgt acctgtggga cggcgacagt
      481 attgcggaaa tccgggaata ccgcgatgat gaactgtaca gcgtacggca cctggtgttt
      541 aacggctttg agctgataag ccagcagtgc agccgggtac ggcagccgca cccgtccgtg
      601 gccccgcagt gggtgacacg aacgaatcat gcggtgaacg acctgacggg ccgtccgctg
      661 atgctcttta acagtgaagg taaaaccgtc tggcggccgg gacagaccag cctgtggggg
      721 ctggcactca gcctgcccgc agacacagac tacccggacc cgcgcgggga gcgggacgcg
      781 gaagccgacc ccggcctgct gtatgcagga cagtggcagg atgcagaatc ggggttgtgc
      841 tataaccggt tccgatatta cgagccggaa accgggatgt acctggtgag tgatccactg
      901 gggttgcagg gaggggagca gacttaccgg tatgtgccga atccttgtgg gtgggttgat
      961 ccgctgggat tggctgcaag ttctaaaatc agcagtttga tggactatat tggcgatggt
     1021 cgtcgtgtta gtgggcatac gggtttcctg gatggggttc gtttatcacg tagtcaaata
     1081 aacaatattg ctaaagaaat ggagaagcta ggaattaaag taataaggaa agcagataaa
     1141 tatttgccac caaatgctag ggcagctttt gattatggtc ttcgcaatat ttatcttagg
     1201 aaaaatgcta ccttatatga ggtgtatcat gaagtgattc atgctaagc
//
LOCUS       DBFIFM010000426         1236 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.436, whole genome shotgun sequence.
ACCESSION   DBFIFM010000426 DBFIFM010000000
VERSION     DBFIFM010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.436"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..853
                     /locus_tag="N7C74_004976"
     CDS             <1..853
                     /locus_tag="N7C74_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU2434645.1"
                     /translation="LAVLDYGGFTEASKRLFMTQSAVSQAIAALEQELGVNILLRDRR
                     KAIQLTTAGHRIVQHLRAINREVNAVKEIAEQEKQNPQRTLRLGCFPSVCACILPVVI
                     RYFEIHHPNIKIIPFEENSTAIIDSLQNESIDAGFVHFPVSGMYSVPIYQDKFTVVLP
                     PNHALAKNSTITVEELMGEPLIISKGRYELSIMALFKEKNITPQIKYEFNHPDTAISF
                     IRQGLGIALLPELTLKTIADELCSVPLEPTFYRQISLLAKEKPVEGSPLFLLQMCTEQ
                     LVVSGKI"
BASE COUNT          370 a          258 c          264 g          344 t
ORIGIN      
        1 actcgccgtg ctggattacg gtggctttac tgaagccagc aagcgattgt tcatgacgca
       61 gtcggcggta agtcaggcaa ttgctgccct cgaacaagaa cttggtgtga atatactgtt
      121 acgcgatcgc cgtaaagcta tacaactgac cacagcaggt caccgcattg tgcaacatct
      181 gcgcgccatt aatcgggaag ttaacgcagt aaaagagata gccgagcagg aaaagcagaa
      241 ccctcaaaga acgctgagac ttggttgttt ccccagcgtc tgcgcctgta tcttaccggt
      301 tgtgattcgc tactttgaaa ttcatcatcc gaatataaaa attattcctt ttgaggaaaa
      361 tagcacagcg attattgatt cgcttcagaa tgaatccatt gatgccggtt ttgttcattt
      421 tccggtgagc ggaatgtact ccgtgccgat ttatcaggat aaattcaccg tagtgttacc
      481 gcccaaccat gctctggcaa aaaacagcac tattacagtg gaagaattaa tgggtgaacc
      541 attaatcatt agtaaaggac gctatgaatt aagcattatg gcactattta aagaaaagaa
      601 tattacccca cagataaaat atgaatttaa tcacccggat acagcgatca gttttattcg
      661 acagggatta ggcatcgcct tattaccaga gttaacgctg aaaaccattg cagatgaatt
      721 gtgttccgtt cctctcgaac caaccttcta tcgacagatt tcgttgctgg ctaaagaaaa
      781 gccggtagaa ggcagtccac tgtttttact acaaatgtgt acggaacaac tagtggtgag
      841 tggaaaaatt tgatgtaaga catgagattg atgacaaatg caagactgcc tgattcgcca
      901 cacttagttg tactatagcc tttaccaggc gtaataattc gttttcacct gattttggcc
      961 cactttgtga attggcgata tatttatcca gttgggagaa gtgacaatga ctccaggtag
     1021 aaaccggtat gaagcgcccg actcactgtc aaaaccgatt gttgagagta atgacactcc
     1081 ggcgaacagt acacagttta ctctggagcc attctcaatc ttcaagtggg atacctaata
     1141 acttgtcagg gaagttatca atatctgttt gttacttatt accgtattta ttaacaataa
     1201 tgatactttc taacatgcgt aaaggtcttc gtaata
//
LOCUS       DBFIFM010000427         1225 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.437, whole genome shotgun sequence.
ACCESSION   DBFIFM010000427 DBFIFM010000000
VERSION     DBFIFM010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.437"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..>1225)
                     /gene="xanQ"
                     /locus_tag="N7C74_004977"
     CDS             complement(<1..>1225)
                     /gene="xanQ"
                     /locus_tag="N7C74_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HGU2434646.1"
                     /translation="SDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPALIVGAALQL
                     SAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIALGSSMKSD
                     GFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGLSLIKVGII
                     DFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGLCVGYIASL
                     CLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGDITATAMVS
                     RRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGVASRYVGRT
                     IAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGLKRRETLIV
                     ATSLGLGLGVSYDPEI"
BASE COUNT          380 a          313 c          302 g          230 t
ORIGIN      
        1 aatttcggga tcgtaggaga cgccaagtcc taaacctaaa gaagtggcga caataagtgt
       61 ttctcggcgc tttaaaccgt tggtgatgat gatgcgaatc ccagcgatgg caatcatgga
      121 aaacatcaac gtcattgcgc ctcccagaac tgccgaggga atggtcgtga agaagccgcc
      181 aatcatcgga aacaaaccga ggataaccag cattaccgcg atggttcgcc cgacataacg
      241 tgaagcgacg ccagtcatct gaataacccc attattttgc gcaaacgtgg ttaagggtaa
      301 tgacccgaca gcggaggcga taacagaaac cagaccgtct gccagcacgc cgcctttcag
      361 tcgggactga tactcttctc cctgaatggg tcggcgggaa accattgccg tggcggtgat
      421 atcgccgaca gcttccagca cgctaagcag ataaatcgtg ccaaccacca ggaactgatg
      481 gaagctaaaa ctaaagccgt atttgaacgg atgtgggatg gtgattaacg gcaaattgcg
      541 catactgctg aaatccacca tgcccaggca taacgacgcg atataaccga cgcatagccc
      601 aatggcgatc cctcccatgc gtagcaacgg actgcgacag cagttaaagc cgatcaccac
      661 aatcaaaacc aataaaccaa cgccgagatg ttcgtaattg ccgaaggtac cgctgctttt
      721 ggcagcaaat cctccaccaa aatcgataat gccgacttta atcaggctta agccgatcat
      781 cagtaccaca ataccgctga cagtaggcgt aataacccga cgtaaatagg gcaagataaa
      841 tgaagatccg acaaccagaa atgcgccaac gaaggagacg ccgaggagcg acgacatgat
      901 taactcttcg tgaaaaccgt cgcttttcat gctgctgccc agcgcaatca tgaccgtaac
      961 aaatgaaaaa ttgactgact gaattgagag taggccagaa ccgacgatgc catagcggtt
     1021 tacttgtaac caggtaccaa taccagaggc gatcatcgcc attgaaacaa gataggcagt
     1081 tgtttcagcg gaaagctgta aggccgcccc cacgattaac gcgggggtta ccatcggaac
     1141 gaaaattgcc aacagatggg taatggcacc aacgagagcc tgatgaaagg gagggcgatc
     1201 ctccagttca aatataaggt cagaa
//
LOCUS       DBFIFM010000428         1225 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.438, whole genome shotgun sequence.
ACCESSION   DBFIFM010000428 DBFIFM010000000
VERSION     DBFIFM010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.438"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..341
                     /gene="stpA"
                     /locus_tag="N7C74_004978"
     CDS             <1..341
                     /gene="stpA"
                     /locus_tag="N7C74_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HGU2434647.1"
                     /translation="IDVLEEMLEKFRVVTKERREEEEQQQRELAERQEKISTWLELMK
                     ADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDVNGETKTWTGQGRTPKPIAQALA
                     EGKSLDDFLI"
     gene            complement(394..912)
                     /gene="ygaP"
                     /locus_tag="N7C74_004979"
     CDS             complement(394..912)
                     /gene="ygaP"
                     /locus_tag="N7C74_004979"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HGU2434648.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRRDQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQ"
     gene            complement(922..1221)
                     /gene="ygaV"
                     /locus_tag="N7C74_004980"
     CDS             complement(922..1221)
                     /gene="ygaV"
                     /locus_tag="N7C74_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGU2434649.1"
                     /translation="MTELTQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          322 a          300 c          305 g          298 t
ORIGIN      
        1 ccattgacgt tcttgaagaa atgctcgaaa aattcagggt tgtcactaaa gaaagacgtg
       61 aagaagaaga acagcagcag cgtgaactgg cagagcgcca ggaaaaaatt agcacctggc
      121 tggagctgat gaaagctgac ggaattaacc cggaagagtt attgggtaat agctctgctg
      181 ctgcaccacg tgctggtaaa aaacgccagc cgcgtccggc gaaatataaa ttcaccgatg
      241 ttaacggtga aactaaaacc tggaccggtc agggccgtac accgaagcca atcgctcagg
      301 cgctggcaga aggtaaatct ctcgacgatt tcctgatcta attactgagg ccggatgtcg
      361 ctgctagggc gcgtccggcg tcgcttctca agctcattgg ttccacggca tcttatctaa
      421 caaccttgcc atcccacaaa aaccgctaat tcctgcaaac agtaacccgg caccaacaaa
      481 gccgcttaat aagaagaaac cgctatttac ggtataaccc agtataacgc cgattaatat
      541 taaaccaccc gcagcgatct gcacctggcg cattaacggc aagggttgag atttatttac
      601 cgctactggt aatcctgctt ttttccagcc atcaatccca tcttcgagta aaaagatttc
      661 tgcgggggcg gcaatcgctg ctaatttatc ggcgttatta ctggtgcgtt tacctgcctg
      721 gcagtggaat ataatttgat cgcgacgtaa tttagccggg agacctgact gttccagcac
      781 ggataatgga gccagatctg cttcaggaat atgttcacga agatattcat cagcgtcacg
      841 aatatcgatt aactttgcgc cgcgtgcgat taattcttgt gcatcatgcg gcgaaatggt
      901 tgtcaaagcc atagtgactc cttacggaca atagacattt ttcagggtgg cgataatggc
      961 atttaccgcc tcatttttaa tggaatatag aatgcgttgg gcatcccgtt ggctgttgat
     1021 aagcccttcg tcccgcatac gagcgagatg ctgtgatgtc gcagaggcgc tcagtccggt
     1081 aatgcgcgtc agctctcccg cgctggtgcc gggggaaccg ctaagcatgc acagaatcag
     1141 caaccgttta gggtggctca ttgctttcaa taaggccgct gcctgttcag cactggcctg
     1201 taattgcgtg agttcggtca tagtt
//
LOCUS       DBFIFM010000429         1225 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.439, whole genome shotgun sequence.
ACCESSION   DBFIFM010000429 DBFIFM010000000
VERSION     DBFIFM010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.439"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..489
                     /locus_tag="N7C74_004981"
     CDS             <1..489
                     /locus_tag="N7C74_004981"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGU2434650.1"
                     /translation="FNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQGNALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCE
                     SP"
     gene            587..>1225
                     /locus_tag="N7C74_004982"
     CDS             587..>1225
                     /locus_tag="N7C74_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HGU2434651.1"
                     /translation="MLIIDQSAITRFYWQLYYNFLGCITLMRGETCMVFNKKMFVLII
                     IPGILGVLLSFAMSVFQMNRDTTITAGILLKQLDNVTQIVKHTTKLTSILVMKPCKDI
                     LEQLIANGALTPYVRTTGLIENNFQICSSVSGFKKMNVNDVYGTSFHNKNKESRIVSI
                     SGTSFVPGKTAIVFLMPIGNDMTAFSIVESRYIYDLMDVLDDENDDSFSLRFT"
BASE COUNT          378 a          217 c          270 g          360 t
ORIGIN      
        1 tttaacagaa attatttttt taatgaactg acagttcaat cagcatcagc caaaaaaacg
       61 ccttattgcg tcatgattat ggatatcgac cacttcaaaa aagtcaacga cacctggggg
      121 catccggttg gcgatcaggt gataaaaaca gtggtgaata tcattgggaa aagcatacga
      181 ccagatgacc ttttagcgcg cgtcggcggc gaagagtttg gcgtcttgct gacggacatc
      241 gatactgaac gtgcgaaagc tttagcggaa aggattcggg aaaatgttga gcgtttaact
      301 ggcgataatc ctgaatacgc tataccacaa aaagtgacga ttagtattgg cgctgttgtg
      361 actcagggaa atgcgttaaa cccgaatgag atctatcgac tggctgataa cgcactatac
      421 gaggcgaaag agaccgggcg taataaggtg gttgtgaggg atgtggtgaa tttttgtgag
      481 tcaccataaa gcggcatttt agcgggtgat tttggtaagc gctaaatagc tattcgcaga
      541 catgcgtaac aattttcaga aatatcatag tactatatga tcttaaatgc ttatcattga
      601 tcagtctgct atcacccgct tttactggca attgtactat aatttcttag gttgcatcac
      661 cctgatgcgt ggggaaacat gtatggtatt caacaaaaaa atgtttgtac tcattataat
      721 accaggcatt ctgggggtgt tactgtcgtt tgctatgtca gtatttcaaa tgaatcgaga
      781 tacgacaatt accgccggta ttctgttaaa acagcttgat aatgttacgc aaattgtcaa
      841 acatacgaca aagcttactt ctattctggt gatgaaaccc tgcaaagata tcctggaaca
      901 attgattgct aacggagcat tgacccccta tgttcgaaca acgggtctta tagaaaataa
      961 tttccaaata tgttcttcag ttagcggttt caaaaaaatg aatgttaacg atgtttacgg
     1021 tacgagcttt cacaataaaa ataaagaatc acgcatagta tcaattagcg gtacaagttt
     1081 tgtaccaggt aaaacagcca tagtgttcct tatgccaata gggaatgata tgacagcttt
     1141 tagtattgta gagtcacgat atatctatga tctgatggat gttcttgatg atgagaatga
     1201 tgattcattt tctcttcgct ttact
//
LOCUS       DBFIFM010000430         1211 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.440, whole genome shotgun sequence.
ACCESSION   DBFIFM010000430 DBFIFM010000000
VERSION     DBFIFM010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.440"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..621
                     /gene="rfaL"
                     /locus_tag="N7C74_004983"
     CDS             <1..621
                     /gene="rfaL"
                     /locus_tag="N7C74_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HGU2434652.1"
                     /translation="VIFIFLILGTLSRGAWLSVLVIGLIWILMFKQWKLLLVGVMVAI
                     IALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGSALDLILENPVIGYGYGNVAYKD
                     VYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGLVSLMMLYCAILKECIKNGVKNK
                     YRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVYAGLLLAMKNK"
     gene            complement(657..>1211)
                     /locus_tag="N7C74_004984"
     CDS             complement(657..>1211)
                     /locus_tag="N7C74_004984"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGU2434653.1"
                     /translation="FYAGRIVPDKGILLLMQSFEKLAAAHKNLKLVVIGDYTEMSKSD
                     KGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPLADLVVIPSQFQEPFCMVAIEAM
                     GAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDILKTLANPELTAVAKQGQD
                     FVFDHYSWDGVTQRFEEVIHNWFE"
BASE COUNT          368 a          231 c          218 g          394 t
ORIGIN      
        1 gttattttta tttttctgat attaggaact ttatccagag gggcctggct ttccgtgtta
       61 gtcattggat taatatggat tctgatgttt aaacaatgga agttactatt agtaggagta
      121 atggttgcca tcattgcatt gtcggttatt ttcacacata aggagatgac cgcaaagcta
      181 acgtataaac ttcaacaaac taatagttct tatcgctatg caaatggtac tcaaggcagc
      241 gcactcgatc taatattaga aaatcctgtt attggttatg gttacggtaa cgttgcatat
      301 aaagatgtct ataataaacg tgtcattgat tatccagaat ggacctttag acaatcaata
      361 gggccacata attttgcgct attcatctgg tttggcactg gtttattagg gctggtaagt
      421 cttatgatgc tatactgtgc aatattgaaa gagtgtataa aaaatggcgt caagaataaa
      481 tatcgctcac catataatgc atattatata atcttactat cttttatagg ttattttgtt
      541 atccgtggaa acgtagaaca aattgaacca aatttattag gcgtttacgc cggcttatta
      601 ttagcgatga aaaacaagta aaaaataaaa aaggctgcat aatgcggcct ttttatttat
      661 tcaaaccaat tatgaataac ctcttcgaat ctttgagtta cgccatccca actgtagtga
      721 tcaaacacaa agtcctgacc ttgctttgca actgctgtta attcaggatt cgctaacgtt
      781 tttaaaatat cgctgcttat ggagtctgca gtcataggtt cttttaaatg gaatcccgta
      841 gtattttctt tgacaaattc cgtcatcccc ccacgcgtac tcactaaaac aggcttacca
      901 gcacccatag cttcaattgc caccatgcaa aatggttcct ggaactggga aggaatgacg
      961 actaaatctg ccagtgggta ataacaatgc atcttttcag gcggtatgct tcctaacata
     1021 atgcaacgat ccttcaaacg ttttgcaatt tccctgacat tgcgttgata tgcacctttg
     1081 tcacttttac tcatttctgt ataatcacca atcacaacta attttaaatt tttgtgagca
     1141 gcggccaatt tttcgaatga ttgcatcaac agcaatatgc ctttgtcagg gacgatacgc
     1201 ccagcataaa a
//
LOCUS       DBFIFM010000431         1198 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.441, whole genome shotgun sequence.
ACCESSION   DBFIFM010000431 DBFIFM010000000
VERSION     DBFIFM010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.441"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..607
                     /locus_tag="N7C74_004985"
     CDS             <1..607
                     /locus_tag="N7C74_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HGU2434654.1"
                     /translation="EDELLQGKLEPTNEPYAIAKIAGIKLCESYNRQFGRDYRSVMPT
                     NLYGPNDNFHPSNSHVIPALLRRFHDAVENNSPNVVVWGSGTPKREFLHVDDMASASI
                     YVMEMPYDIWQKNTKVMLSHINIGTGIDCTICELAETIAKVVGYKGHITFDTTKPDGA
                     PRKLLDVTLLHQLGWNHKITLHKGLENTYNWFLENQLQYRG"
     gene            610..1119
                     /locus_tag="N7C74_004986"
     CDS             610..1119
                     /locus_tag="N7C74_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HGU2434655.1"
                     /translation="MFLHSQDFATIVRSTPLISIDLIVENEFGEILLGKRINRPAQGY
                     WFVPGGRVLKDEKLQTAFERLTEIELGIRLPLSVGKFYGIWQHFYEDNSMGGDFSTHY
                     IVIAFLLKLQPNILKLPKSQHNAYCWLSRAKLINDDDVHYNCRAYFNNKTNDAIGLDN
                     KDIICLMRQ"
     gene            1101..>1198
                     /locus_tag="N7C74_004987"
     CDS             1101..>1198
                     /locus_tag="N7C74_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HGU2434656.1"
                     /translation="MSDAPIIAVVMAGGTGSRLWPLSRELYPKQFL"
BASE COUNT          369 a          215 c          250 g          364 t
ORIGIN      
        1 ggaagacgaa ttattacaag ggaaacttga gccaacaaat gaaccttatg ctatcgcaaa
       61 aattgcaggt attaaattat gtgaatctta taaccgtcag tttgggcgtg attaccgttc
      121 agtaatgcca accaatcttt atggtccaaa tgacaatttt catccaagta attctcatgt
      181 gattccggcg cttttgcgcc gctttcatga tgctgtggaa aacaattctc cgaatgttgt
      241 tgtttgggga agtggtactc caaagcgtga attcttacat gtagatgata tggcttctgc
      301 aagcatttat gtcatggaga tgccatacga tatatggcaa aaaaatacta aagtaatgtt
      361 gtctcatatc aatattggaa caggtattga ctgcacgatt tgtgagcttg cggaaacaat
      421 agcaaaagtt gtaggttata aagggcatat tacgttcgat acaacaaagc ccgatggagc
      481 ccctcgaaaa ctacttgatg taacgcttct tcatcaacta ggttggaatc ataaaattac
      541 ccttcacaag ggtcttgaaa atacatacaa ctggtttctt gaaaaccaac ttcaatatcg
      601 ggggtaataa tgtttttaca ttcccaagac tttgccacaa ttgtaaggtc tactcctctt
      661 atttctatag atttgattgt ggaaaacgag tttggcgaaa ttttgctagg aaaacgaatc
      721 aaccgcccgg cacagggcta ttggttcgtt cctggtggta gggtgttgaa agatgaaaaa
      781 ttgcagacag cctttgaacg attgacagaa attgaactag gaattcgttt gcctctctct
      841 gtgggtaagt tttatggtat ctggcagcac ttctacgaag acaatagtat ggggggagac
      901 ttttcaacgc attatatagt tatagcattc cttcttaaat tacaaccaaa cattttgaaa
      961 ttaccgaagt cacaacataa tgcttattgc tggctatcgc gagcaaagct gataaatgat
     1021 gacgatgtgc attataattg tcgcgcatat tttaacaata aaacaaatga tgcgattggc
     1081 ttagataata aggatataat atgtctgatg cgccaataat tgctgtagtt atggccggtg
     1141 gtacaggcag tcgtctttgg ccactttctc gtgaactata tccaaagcag tttttaca
//
LOCUS       DBFIFM010000432         1197 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.442, whole genome shotgun sequence.
ACCESSION   DBFIFM010000432 DBFIFM010000000
VERSION     DBFIFM010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.442"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            1..1197
                     /locus_tag="N7C74_004988"
                     /pseudo
     CDS             1..1197
                     /locus_tag="N7C74_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
BASE COUNT          422 a          239 c          227 g          309 t
ORIGIN      
        1 cttgtggagt ttatcctcag gatttaaaag acagagaaat tatatcaact tttcacgcag
       61 aaaaagtcat taatgatctg ttaaaaaacg attataaaat ttccctttct ccagatacaa
      121 cttaccgaga gctgaataaa gctgcacagc gtagcattac agcgccagac aggataggag
      181 aagggaaaat atgggtttat caacgagata caatggttga aagaggtgat aacagcggtg
      241 ttcatcagta tggtccagct gaacatttca ctcacattat atctgacaaa ccgtccccaa
      301 aagataagta tgttgcatat gctattaaca ttcctgacta tgagctagca gccgatgtat
      361 ataatattaa cgtgacgtca ccttccggac agcaagaaac atttaaaata ttaatcaatc
      421 cagaacatct acggcaaaca cttgagcgta aatcccttac tgctgttcag aaatcacaat
      481 gtgaaatcat cacccccaaa aaacctggcg aagcgattct tcatgctttt aatgccacct
      541 accagcaaat cagggaaaat atgtctgagt ttgcacgttg ccattatggg tatatacaaa
      601 tccctccagt gacaactttc cgcgccgacg gaccagaaac tcccgaagaa gaaaaaggtt
      661 actggtttca cgcttatcaa cccgaagatc tttgtaccat ccataatcca atgggagatt
      721 tgcaggattt tatcgcattg gttaaagatg ctaaaaaatt tggtatcgat atcattcctg
      781 attatacctt taactttatg ggaatcgggg gtagtggtaa aaatgacctg gattatccct
      841 ctgctgatat acgagcgaag atcagtaaag atatagaagg tggtatccct ggctattggc
      901 aaggtcaggt tttgattcca tttactatag atccagtaac aaaagaacgt aaacaaatcc
      961 atccagaaga tatacatctc acagcaaaag acttcgaagc aagtaaagat aatatttcta
     1021 aggatgaatg ggaaaacctc catgcattaa aagaaaagcg tttaaatgga atgcctaaaa
     1081 ccccccccaa aagtgactag gttattatgt tgcaaaatca atacgttcgt gaaatgcgaa
     1141 aatatggcgt acgaggttta cgttacgatg cagcaaaaca ctcaaaacat gaacaaa
//
LOCUS       DBFIFM010000433         1192 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.443, whole genome shotgun sequence.
ACCESSION   DBFIFM010000433 DBFIFM010000000
VERSION     DBFIFM010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.443"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..441
                     /locus_tag="N7C74_004989"
     CDS             <1..441
                     /locus_tag="N7C74_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU2434657.1"
                     /translation="DEKEGNHTTAYRDGAGIWTICRGAILVDGKPVVPGMKLSKEKCD
                     QVNAIERDKALEWVERNIKVPLTEPQKAGIASFCPYNIGPGKCFPSTFYRRINAGDRR
                     GACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQESALACWGIDR"
     gene            597..779
                     /locus_tag="N7C74_004990"
     CDS             597..779
                     /locus_tag="N7C74_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434658.1"
                     /translation="MGKYKRILLRLTMKNGLELKAPVTDDVSRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            792..923
                     /locus_tag="N7C74_004991"
     CDS             792..923
                     /locus_tag="N7C74_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434659.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAICFSTAWRVLTLSWLVG"
     gene            931..>1192
                     /locus_tag="N7C74_004992"
     CDS             931..>1192
                     /locus_tag="N7C74_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HGU2434660.1"
                     /translation="MNRVLCVVIIVLLVACGVLSLGLNHYRDNAITYKAQRDKKASEL
                     KLANVTITDMQVRQRDVAALDARYSRELADARAENETLRADVA"
BASE COUNT          299 a          246 c          355 g          292 t
ORIGIN      
        1 gacgaaaaag aaggtaacca caccacggca taccgtgatg gtgcgggtat ctggaccatc
       61 tgccgcggtg ccatcctggt ggatggtaaa cctgtcgttc cgggcatgaa gttgtcgaag
      121 gaaaaatgcg accaggttaa cgccattgaa cgtgataagg cgctggagtg ggtggagcgc
      181 aatattaaag taccgctgac cgaaccccag aaagcgggga tcgcgtcatt ctgtccgtac
      241 aacattggtc ccggtaagtg tttcccgtcg acgttttaca gacgaattaa tgcaggtgat
      301 cgcaggggag cgtgtgaggc gattcgctgg tggattaagg acggtggcag agactgccgt
      361 attcgctcaa acaactgcta cggtcaggtt tcacggcgtg accaggagag cgcgctggcg
      421 tgctggggaa ttgacagata agcagaatat tttgctgaaa aatgcggttt gctcacactg
      481 gcggataaca cgaaatcctg cgaactggca aaaactaagt gaataaaagt aaaaccccgt
      541 ttgttggccg caaacggggt tttgtgtttc tgaccttgag taaggcaagg gagaacatgg
      601 gaaagtataa acgaattctg ttgagattga ctatgaaaaa cggccttgaa ctgaaagcgc
      661 ctgtaactga tgacgtcagc agagcgctgg cttttgctat taagtgggtg gcggtcggta
      721 ttgctgtgtc tccgatgctg tatgggctgg caaaactggt cattgcgttg aaatcgtgaa
      781 gaggattaag catgtcagac aagctcataa cgctggcgaa gatcctctgt gtaatcgtcg
      841 gcatttcatt ttcactaatg ctggttgcta tttgcttttc cactgcctgg cgcgtcttaa
      901 ctttgtcatg gctggtgggg tgagggggat atgaaccgtg ttctgtgtgt ggtgattatt
      961 gtcctgctgg tagcctgtgg tgtgcttagt ctggggctga atcattaccg cgataacgcc
     1021 attacctaca aagcgcaacg cgataaaaaa gccagtgagc tgaagctggc gaacgtgaca
     1081 attactgata tgcaggtacg ccagcgtgat gtcgctgcac ttgatgccag atactcgagg
     1141 gaattagccg atgcgagagc tgaaaatgaa actctgcgtg ctgatgttgc cg
//
LOCUS       DBFIFM010000434         1187 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.444, whole genome shotgun sequence.
ACCESSION   DBFIFM010000434 DBFIFM010000000
VERSION     DBFIFM010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.444"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..60)
                     /locus_tag="N7C74_004993"
     CDS             complement(<1..60)
                     /locus_tag="N7C74_004993"
                     /inference="COORDINATES: protein motif:HMM:NF027269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HGU2434661.1"
                     /translation="MHRWISQNNIRLPCGAFFIS"
     gene            complement(35..223)
                     /gene="ymcF"
                     /locus_tag="N7C74_004994"
     CDS             complement(35..223)
                     /gene="ymcF"
                     /locus_tag="N7C74_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HGU2434662.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            complement(234..446)
                     /gene="cspG"
                     /locus_tag="N7C74_004995"
     CDS             complement(234..446)
                     /gene="cspG"
                     /locus_tag="N7C74_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HGU2434663.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            732..944
                     /gene="cspH"
                     /locus_tag="N7C74_004996"
     CDS             732..944
                     /gene="cspH"
                     /locus_tag="N7C74_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HGU2434664.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT          349 a          246 c          238 g          354 t
ORIGIN      
        1 agatataaaa aaggctccac aagggagcct gatattattt tgtgaaatcc agcggtgcat
       61 ggtcagtgcg tatttgcagc gcgacgttat caaaggtgat catcgtcgat ttacaaaaaa
      121 tacacttagc gcccaaaggg tttcgctccg tcacatcgaa tgccgatgtg cggtactgtg
      181 aaccatgaca acatggacag cgaaaatgga gatgttgagt aataacggca accttagagc
      241 gtaactacgt tcgctgccgc ggggccacgt tgaccctgct caatagaaaa ttcaactttc
      301 tgattttcgt tcagcgtgcg gaattcattg ctctggatgg cggtgaaatg gacgaaaacg
      361 tctttgctgc catcatcagg agtgataaag ccaaaacctt tatctgcgtt aaaccatttt
      421 actaaaccag tcattttatt agacatattt tacttccttc attttgggcc tttcggcaaa
      481 cagggcttta atacagaaac tacttagtgt tcaggagaga gactcaaaag aaggggataa
      541 tgcctgataa tgagaactgc tttagtaaac tactttgtat gctgtctgtc tttccaaccg
      601 acgcagctat taacgcatga taattgagat taagcaaatt ttattttagc cgtccggcgg
      661 tcttaaatat cgctggcttt attttattaa ccccgtatag tgcagcgcag tattttcagt
      721 aaggaatttg tttgtcccgt aaaatgacag gaattgtcaa aacctttgat cgcaaaagcg
      781 gcaaaggatt cattatcccc tccgacggtc gtaaagaagt tcaggtccat atttccgcat
      841 tcactccccg cgacgcagaa gtgcttatac caggattacg cgtggaattt tgccgcgtaa
      901 atggcctgcg aggaccaaca gcggcaaatg tttatctctc ttaatatgac gctgcttgtt
      961 taaagctgac tggttttatt ggcatagttc aatagtcaac accatgtatt ataacacaag
     1021 ttactttttc accggaaaac taatccattt aaccgaatat ctctacaact aatagttata
     1081 aatgccactt aacagcgagt tatagtacca cttgaaacta tattgccaac aatagttaat
     1141 agctttctta acaattttct tatctttaat ttgtattttg ctaagat
//
LOCUS       DBFIFM010000435         1180 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.445, whole genome shotgun sequence.
ACCESSION   DBFIFM010000435 DBFIFM010000000
VERSION     DBFIFM010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.445"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..253
                     /locus_tag="N7C74_004997"
     CDS             <1..253
                     /locus_tag="N7C74_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGU2434665.1"
                     /translation="SGAVIRGVFDDPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRG
                     DTLTINGEMFWVDRVSPDDGGSCYLWLNRGQPPAVNRRR"
     gene            268..801
                     /locus_tag="N7C74_004998"
     CDS             268..801
                     /locus_tag="N7C74_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2434666.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVKRGDLPVIKLGIRMLGRRPDSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENIDRIRRERLP
                     GELAYALKQQLRIAIKR"
     gene            798..>1180
                     /locus_tag="N7C74_004999"
     CDS             798..>1180
                     /locus_tag="N7C74_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGU2434667.1"
                     /translation="MKHTDIRAAVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAVTAIPALADLITTM
                     VTQGYEYRRDDDMALWSSADLTYSIT"
BASE COUNT          280 a          274 c          359 g          267 t
ORIGIN      
        1 gtccggcgca gtaatacgtg gtgtttttga tgaccctgaa aatatcagct atgccggaca
       61 gggcgtgcgc gttgaaggct ccagcccgtc cctgtttgtc cggactgatg atgtgcggca
      121 actgcggcgt ggtgacacgc tgaccattaa cggcgagatg ttctgggtgg accgtgtttc
      181 tccggatgac ggagggagtt gttatctctg gctcaaccgt gggcaaccac cggcagttaa
      241 ccggcgacga taaacgcagg gtgaattatg gcgataaaag ggcttgatca ggcgattgaa
      301 aatctgagcc gggttcgtaa aaacgccatt ccggcggctt cagcaatggc cattaaccgc
      361 gtggccacaa cggcgattaa tcagtcttca tcacaggttg cccgggagac aaaggtacgc
      421 cggaaactgg taaaggaacg gtccagactg aaacgggcca cggtcagaaa tccgaatgcc
      481 agaattatcg ttaaacgcgg tgatctccct gtgattaagc tggggatcag gatgctgggc
      541 cgtcgtccgg acagcatact taaagctggt cagcatcggt atcagcgggc atttatccag
      601 cgattaaaaa atggtcgctg gcatgtcatg cagcgtgtgg ccgggaaaaa ccgttacccc
      661 attgatgtgg tgaaaatccc gatggcggcc ccactgaaac aggcgtttga tgagaatatt
      721 gaccgtatcc ggcgtgaacg tctgcccgga gaactggcat acgcgctgaa acaacaactt
      781 aggattgcga taaaacgatg aaacatactg atatccgtgc ggcagtgctg gatgcactgg
      841 agctgcatga acacggggcg acgctgtttg atggtcgccc cgttgttttt gacgaagagg
      901 attttccggc cgtcgcggtt tatctgacgg atgcagagta taccggtgaa gagctggatg
      961 cagatacctg gcgggccaca ctgcatattg aggtgttttt accggcacag gtaccggatt
     1021 cggagcttga tcagtggatg gaaagccgga tttatccggc ggtgactgcg atcccggcac
     1081 tggcagacct gattacgacg atggttacgc agggctatga gtatcgtcgt gatgacgata
     1141 tggcgttatg gagttctgcg gatctgactt attccattac
//
LOCUS       DBFIFM010000436         1162 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.446, whole genome shotgun sequence.
ACCESSION   DBFIFM010000436 DBFIFM010000000
VERSION     DBFIFM010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.446"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..>1162)
                     /locus_tag="N7C74_005000"
     CDS             complement(<1..>1162)
                     /locus_tag="N7C74_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU2434668.1"
                     /translation="VIRTDDERTHHYHYDSQHRLVFYTRIQHGEPLVESRYLYDPLGR
                     RTGKRVWRRGRDLTGWMSLSRKPEVTWYGWDGDRLTTVQTDTTRIQTVYEPGSFAPLI
                     RIETDNGEREKAQRRSLAEKLQQEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSES
                     RAWLAQCGLTVEQLARQVEPEYTPARKVHLYHCDHRGLPLALISEDGNTAWSGEYDEW
                     GNQLNEENPHHLHQPYRLPGQQYDKESGLYYNRNRYYDPLQGRYITQDPIGLEGGWSL
                     YAYPLNPVNGIDPLGLSPADVALIRRKDQLNHQRAWDILSDTYEDMKRLNLGGTDQFF
                     HCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNLFGMYGRKVKLSHSEMI"
BASE COUNT          210 a          387 c          271 g          294 t
ORIGIN      
        1 tcaatcattt cagaatggga tagctttact tttctgccgt acataccgaa cagatttaac
       61 ccgtaatctc taatctcttt ttcataaccc agccctttcg ccgatcggct aacaccagcg
      121 tcatttaatt tagacactcg acaaaatgcc atacaatgga aaaattgatc agtcccacct
      181 aaatttaatc tcttcatatc ttcataagta tcagataata tatcccatgc tctttgatgg
      241 tttagttgat cttttcttct tattagcgct acatctgcgg gacttaaccc taatggatca
      301 ataccattca ccggattcag cggatacgca tacagactcc atcccccctc cagccctatc
      361 gggtcctgag tgatataccg cccctgcaac ggatcgtagt accggttccg gttgtagtac
      421 agccccgact ccttatcata ctgctgcccc ggcagccggt acggctggtg caggtgatgc
      481 gggttctcct cattcagctg gttgccccat tcatcatact ccccgctcca cgccgtattg
      541 ccgtcttcgc tgatgagcgc cagcggcagg ccccggtggt cgcagtggta aagatgaact
      601 tttctcgccg gtgtgtattc cggctccacc tgtctggcca gttgctccac cgtcagcccg
      661 cactgcgcaa gccacgcccg gctttcactg ctcacgcggt ctgcccggat ttcttcctcc
      721 agtctgtcca gcagccgcac cagttcagcc ggaaatacca caccgtgccc gtcctcgctc
      781 ccttcctgct gcagcttctc cgccaggctg cggcgctgcg ctttctcccg ctcaccgttg
      841 tctgtttcga tgcggatgag cggcgcgaag cttcccggct cgtataccgt ctggatacgc
      901 gtggtgtcgg tctgtaccgt cgtcagccgg tcgccgtccc acccgtacca cgtcacctcc
      961 ggtttccgcg acagcgacat ccatcccgtc aggtcacgcc cgcgacgcca cacccgtttc
     1021 cccgtccggc ggcccagcgg gtcgtagagg tagcggctct cgaccagtgg ctcgccatgc
     1081 tgtatccgcg tgtagaacac caggcggtgc tggctgtcgt agtggtagtg gtgggtccgc
     1141 tcgtcgtccg tccgtatcac ac
//
LOCUS       DBFIFM010000437         1156 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.447, whole genome shotgun sequence.
ACCESSION   DBFIFM010000437 DBFIFM010000000
VERSION     DBFIFM010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.447"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(1..552)
                     /locus_tag="N7C74_005001"
                     /pseudo
     CDS             complement(1..552)
                     /locus_tag="N7C74_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131658.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
     gene            complement(665..934)
                     /locus_tag="N7C74_005002"
     CDS             complement(665..934)
                     /locus_tag="N7C74_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU2434669.1"
                     /translation="MNILKKLMQRLCGHGKHDDREDGELLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            complement(936..>1156)
                     /locus_tag="N7C74_005003"
     CDS             complement(936..>1156)
                     /locus_tag="N7C74_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU2434670.1"
                     /translation="RLGPASIIETNSHGWFPGTDGALITGLTFLAPKDTTRVQGFFQH
                     LQVRFGDGPWQDVKGLDEVGSDTGRTGE"
BASE COUNT          309 a          314 c          219 g          314 t
ORIGIN      
        1 ttcccttgat aggatcataa acacatttat caggacttat aatcattgat gccgttattg
       61 gttctcgggt cagtggatga tatgagcctt cactagctaa acgagaaaat gcatcaaaat
      121 caaataagca gcatactgcc gaactatctg aatttttgac aaacactcct tcttcgggtc
      181 tctctagtgt aattggacac tgaatagact ctggcgggca ctggaaattt cctgaactaa
      241 caggaaactt acattgagat attttgtcct gaatactctc ttgcgattgt gcttcacctg
      301 agtccgaaag catactgagc atcctatcgc gaagagcttc agggccgtta ttaaacccat
      361 aaaggaataa ctcagtaagc aatcccccac tactgccgct ggatagaaaa cgcccatcgg
      421 tttcattaaa tacaatgctg acagtctcat tacctaatgt aaactcccct cgaccgctag
      481 atgctacttg agctcgaagg acattcaatc cattaaaaat gagcttgatc taatctccga
      541 atttaacggc attataaaac tccctatttt aatttaaact ccagacttaa atagctttaa
      601 caaacatctg ccttacatga caacaaaaac cggagccgga ctccggtttt gtgaagctgt
      661 cgggttactt catcccgcca atattttccc acgtcccgtc agcacgcagg atttgcagcg
      721 gtcttaccac gcactgtatc tgctttttat ccgcatccag tatcaccacc tgcgtgatta
      781 ccctgtcctg ctccggaata ataccattct catcggactc caggatgtct gccggcccca
      841 gacgcagttg tgctgtaagt aactccccgt cttcacggtc atcatgcttt ccgtgcccgc
      901 acagacgctg cataagtttt tttagtatgt tcatgtcatt ctcctgttct gcctgtatca
      961 ctgcccactt catcaagccc cttaacatcc tgccacggcc cgtcaccaaa cctgacctgc
     1021 aaatgctgaa aaaaaccctg aacccgtgtg gtatctttgg gggcaagaaa ggtcagtccg
     1081 gtgatgagcg caccatctgt acccgggaac cagccatggc tgtttgtctc aataatgctc
     1141 gccggcccca gacgaa
//
LOCUS       DBFIFM010000438         1152 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.448, whole genome shotgun sequence.
ACCESSION   DBFIFM010000438 DBFIFM010000000
VERSION     DBFIFM010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.448"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            191..1135
                     /locus_tag="N7C74_005004"
     CDS             191..1135
                     /locus_tag="N7C74_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434671.1"
                     /translation="MSPDNIGSAVNRFSGWPSNDSLKTDKESMTSPVTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNRDEESHYLAFE
                     YNVDTSDRITVSAYNEKKPGCYGQCLVIQDDETDLFNLIKIKIFEARHNYVAQLMSDP
                     EFMFKFSYSAQQNRLEPHLLVPAYFPLKTNEPVTQEDVLLLYRFFKMNDDFNKLTSDE
                     YMSILTPLMKCERSVHDNNRYVTGKDTLLLDYPPSGNQIHFHVFPDESATLVLYFSNN
                     DIECFVFERDIPSQYRFFKMFTNLALVIDQLTEANKIL"
BASE COUNT          362 a          232 c          209 g          349 t
ORIGIN      
        1 gttattgttt cattagtaat aatcacttaa atgtatcgat gaaacaggta tgacttaccg
       61 ggagtgattg tttctgagtg aacagaggcc actttcgcct gataaatgaa aaatgagcga
      121 tttttgcaag ttagtttttc tatagccgag tacgctcagc gcctcgcttg attacaatga
      181 ggttgctgct atgagtcctg ataatatcgg ttcggctgtc aatcgcttct caggttggcc
      241 atctaatgat tctctaaaaa cagataaaga atcaatgaca tcacccgtga ctgtgcaggt
      301 aataaagatg attgccgcca tccttgaccc acatcggata gtcgttggaa ttgatacact
      361 ttatacaacg cagcgcgaaa catttacctc agagagtcaa cagctcattg aatcagcatt
      421 gcaaacactt catgtgatga atttaaataa tctggaaaca aatcgcgatg aagaaagtca
      481 ctatctggct tttgaatata acgttgacac ctctgacaga ataaccgttt ctgcttataa
      541 tgaaaaaaaa cctggctgct atggccaatg tcttgtaata caggatgacg aaacagactt
      601 atttaacctg ataaaaatta aaattttcga agccaggcat aattatgtcg ctcaattgat
      661 gagcgatccg gaatttatgt tcaaattcag ttatagcgct caacaaaata gattagaacc
      721 tcatcttctg gttcccgctt atttccctct aaaaacaaac gaaccggtta cgcaagagga
      781 tgtactgtta ctttatcgtt tctttaaaat gaatgatgat tttaataaat taacatcaga
      841 tgaatacatg agtatcctga cacctctgat gaagtgtgag cgtagcgtcc acgacaacaa
      901 caggtatgtg acgggcaaag atacccttct tctggactat cctccttctg gtaaccagat
      961 acatttccat gttttccctg acgaatcagc aacactagtc ctttattttt caaacaatga
     1021 cattgaatgt ttcgtctttg aaagagatat tccgagccag tatcgctttt tcaaaatgtt
     1081 taccaatctg gcactggtta tagatcagtt aaccgaagcc aataaaatac tttaattttc
     1141 tatatatccc ct
//
LOCUS       DBFIFM010000439         1146 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.449, whole genome shotgun sequence.
ACCESSION   DBFIFM010000439 DBFIFM010000000
VERSION     DBFIFM010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.449"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(125..196)
                     /locus_tag="N7C74_005005"
                     /pseudo
     CDS             complement(125..196)
                     /locus_tag="N7C74_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
     gene            190..1146
                     /locus_tag="N7C74_005006"
                     /pseudo
     CDS             190..1146
                     /locus_tag="N7C74_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          352 a          277 c          267 g          250 t
ORIGIN      
        1 agaagagggc gataacatac atttcaacct tcaaaataaa cctatctaat tattcctaac
       61 agacatcccc catacatgac aacaaaaacc ggagccggac tccggttttt gtgaagctat
      121 cgggttactt catttcgcca atattttccc acttcccgtc agcacgcagg atttgcagcg
      181 gtcttactac acactgaccc gaggacgaaa aaagagttcg ggctgggaag agacagaaga
      241 gtagcaattt cagaagcatt cacaccaaca ttgagctttt cagcgaaagt gggcacgaat
      301 cattgctgga cagggttaaa ggcactgact caatcactct tcatacgtgg cttgatcgat
      361 atgaaacaat cctcagctag agggggagca agccgaaaac attactcgac tacgccagca
      421 aaatcagggc aattcgaaga aaattgccgg acaaaccgct cactgacata tcaacgaaag
      481 aggtggcagc aatgctaaac acctacgtcg cagaaggtaa agcggtttcc gcaagagtaa
      541 tcaggtcaac ccttgttgac gtttttcgag gggcaatagc cgaggggcat gtggcaacga
      601 atccagtaac aacaacccgt gcagcaaagt cagaagtaag gcgctcaagg ctgacagcta
      661 atgagtatgt cgctatttac catgctgctg agcacctccc catctggctg aggctgtcaa
      721 tggatttagc tgtcgttaca gggcagagag tgggcgattt gtgcagaatg aagtggtcag
      781 acataaacga tggtcatctt cacattggac agagtaaaac aggagccaaa attgccattc
      841 cgctggctct aaccattgac gcactcgaca tctcactggt tgatacacta cagaaatgca
      901 gggaggccag cagcagtgaa acaataatcg catcaaccta tcacgaacca ctttctccag
      961 ccacagtatc acggtattta acaaaggcgc gaaatgcatc cgggatctcg tttgatggag
     1021 acccaccgac atttcatgaa ctacgtagtc tgtccgcgag gctatatcgg aaccagattg
     1081 gctacaagtt tgcacaacgt cttcttggac ataaatctga ttcaatggcg gcgcattata
     1141 gggaca
//
LOCUS       DBFIFM010000440         1139 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.450, whole genome shotgun sequence.
ACCESSION   DBFIFM010000440 DBFIFM010000000
VERSION     DBFIFM010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.450"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..>1139
                     /gene="ehxA"
                     /locus_tag="N7C74_005007"
     CDS             <1..>1139
                     /gene="ehxA"
                     /locus_tag="N7C74_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HGU2434672.1"
                     /translation="VDSFSEQLNQLGSFLSSKPRLSSVGGKLQNLPDLGPLGDGLDVV
                     SGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQYILAQRMAQGLSTT
                     AASAGLITSAVMLAISPLSFLAAADKFERAKQLESYSERFKKLNYEGDALLAAFHKET
                     GAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTISGILEASKQAMFE
                     HVAEKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFSRQHAVERAVAITQ
                     QHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQQVFDPQKGTIDLS
                     TGNVSSVLTFITPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWSV"
BASE COUNT          333 a          218 c          276 g          312 t
ORIGIN      
        1 gttgattcat tttctgagca gcttaaccag cttggctcat ttttatccag taaacctcga
       61 ttaagttctg ttggtgggaa attacaaaat ttaccagacc tgggccccct gggggatggg
      121 ctggatgttg tctccggaat tctttctgct gtatcagcaa gctttattct gggaaacagt
      181 gacgcacata caggaacaaa agctgcagcg ggtatcgaac tgacaactca ggttcttgga
      241 aatgttggta aagctgtttc gcaatatatt ctggctcaga gaatggcaca ggggttatcg
      301 acaacagctg caagtgcggg tctgatcaca tcggctgtta tgctggctat cagtcctctt
      361 tctttcctgg ctgctgcaga taaatttgag cgagctaagc agcttgaatc atattctgaa
      421 cgatttaaaa aattgaatta tgaaggggat gctttactcg cagcctttca taaagaaacc
      481 ggagctatag atgcagccct gacaacaata aatactgtcc tgagttctgt atctgcggga
      541 gttagtgcag cctccagtgc atccctcata ggggccccga taagcatgct ggtgagtgca
      601 ttaaccggta cgatatctgg cattctggaa gcatcaaaac aggctatgtt tgagcacgtt
      661 gcagagaaat tcgctgctcg gatcaatgaa tgggaaaagg agcatggcaa aaattatttt
      721 gagaatggat atgacgcaag acatgctgcg tttttagaag actctctgtc tttgcttgct
      781 gatttttctc gtcagcatgc agtagaaaga gcagtcgcaa taacccagca acattgggat
      841 gagaagatcg gtgaacttgc aggcataacc cgtaatgctg atcgcagtca gagtggtaag
      901 gcatatatta attatctgga aaatggaggg cttttagagg ctcaaccgaa ggagtttaca
      961 caacaagtgt ttgatcctca aaaagggacc atagaccttt caacaggtaa tgtatcaagt
     1021 gttttgacat ttataacacc aacatttacc ccaggagaag aagttagaga aagaaaacag
     1081 agtggtaaat atgaatatat gacatctctt attgtaaatg gtaaggatac atggtctgt
//
LOCUS       DBFIFM010000441         1138 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.451, whole genome shotgun sequence.
ACCESSION   DBFIFM010000441 DBFIFM010000000
VERSION     DBFIFM010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.451"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            33..>1138
                     /gene="espG"
                     /locus_tag="N7C74_005008"
     CDS             33..>1138
                     /gene="espG"
                     /locus_tag="N7C74_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspG"
                     /protein_id="HGU2434673.1"
                     /translation="MINGLNNDSASLVLDAAMKVNSGFKKSWDEMSCAEKLFKVLSFG
                     LWNPTYSRSERQSFQELLTVLEPVYPLPNELGRVSARFSDGSSLRISVTNSELVEAEI
                     RTANNEKITVLLESNEQNRLLQSLPIDRHMPYIQVHRALSEMDLTDTTSMRNLLGFTS
                     KLSTTLIPHNAQTDPLSGPTPFSSIFMDTCRGLGNAKLSLNGVDIPANAQKLLRDALG
                     LKDTHSSPTRNVIDHGISRHDAEQIARESSGSDKQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALTLTHERISKASEYNAERSLDTPNACINISISQSSDGNIYVTSHTGVLIMAP
                     EDRPNEMGMLTNRTSYEVPQGVKCIIDEMVSALQP"
BASE COUNT          357 a          242 c          226 g          313 t
ORIGIN      
        1 tgttcattac aaaccagata ggagaatctc tcatgataaa tggacttaat aatgactccg
       61 catctttagt tttagatgct gcaatgaaag ttaattctgg gtttaaaaaa agctgggatg
      121 agatgtcatg cgctgaaaag ttatttaaag tacttagttt tggtttatgg aatccaacgt
      181 acagtcgtag tgaaagacaa tcatttcaag agttgttaac cgttttagag cctgtatatc
      241 cacttcccaa tgaattaggc agagtatctg ctcgtttttc agatggttca tccttaagaa
      301 tttccgtcac taacagcgaa cttgttgaag ccgagattcg cacagcaaat aatgaaaaga
      361 ttactgtgct cctggagtca aacgaacaaa ataggttatt acaatcttta cccatcgatc
      421 gccacatgcc atacattcag gttcatcgtg ccttatctga gatggacctg actgatacta
      481 cctcaatgcg caatctactt ggttttacgt caaaactatc aacaaccttg attcctcata
      541 atgctcaaac agatccgctt tccgggccta caccattcag ctctatcttt atggatacat
      601 gtcgaggact cggcaatgca aagctttcac tcaatggtgt tgatatacct gcaaatgcac
      661 aaaaattgct tcgcgatgca ctaggactta aagacacaca ttcatcacca acccggaatg
      721 ttatagatca tggtatttct cgccatgatg cagagcaaat agcaagagaa agcagcggca
      781 gtgataaaca gaaagctgaa gttgtggaat ttttatgcca tccagaagca gcaacggcca
      841 tatgctcggc tttctatcaa tctttcaatg tgccagcctt aacgttgaca catgaaagga
      901 tctctaaagc cagtgaatac aatgcggaaa gatcattaga tacacctaac gcttgcatta
      961 acatcagtat ctctcaatca tcagatggaa acatttatgt taccagccat actggggttc
     1021 tgataatggc gccagaagac cgccccaacg agatgggcat gttgacgaac aggacttctt
     1081 atgaagtgcc gcaaggtgtg aaatgtataa tcgatgaaat ggtaagtgcg ctacaacc
//
LOCUS       DBFIFM010000442         1134 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.452, whole genome shotgun sequence.
ACCESSION   DBFIFM010000442 DBFIFM010000000
VERSION     DBFIFM010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.452"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(80..156)
                     /locus_tag="N7C74_005009"
     tRNA            complement(80..156)
                     /locus_tag="N7C74_005009"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(120..122),aa:Arg,seq:tcg)
     gene            complement(158..233)
                     /locus_tag="N7C74_005010"
     tRNA            complement(158..233)
                     /locus_tag="N7C74_005010"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(198..200),aa:Ile,seq:cat)
     gene            complement(547..1035)
                     /locus_tag="N7C74_005011"
     CDS             complement(547..1035)
                     /locus_tag="N7C74_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU2434674.1"
                     /translation="MGIRELNLTKEQHEWLNGWLELWGAWVYSGRLEKRMSSVIAKFM
                     ESVEPGRVMTRPMCNDDDGMLISQVVDSVMYIDKKAFGILLSYYAHGSSKHAIASYYH
                     SVARPRKMLCRGGGRIQKPSLATCRREVDEILNASLFMIYPILDSAFKNRKRVEKIKH
                     VA"
     gene            complement(1026..>1134)
                     /locus_tag="N7C74_005012"
     CDS             complement(1026..>1134)
                     /locus_tag="N7C74_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434675.1"
                     /translation="AVKPLKFANQMYIDTGAVFCGNLTLIQVQGEGAWA"
BASE COUNT          301 a          279 c          248 g          306 t
ORIGIN      
        1 actggattcg aaccagtgac cgattgctta gaaggcaatt gctctgtcct gctgagctaa
       61 caacgcagaa taccgataat ggaccaccac cggggactcg aacctcgcgc aaccagcttc
      121 gaaggctgac gctctatccc gatgagctaa tggcggttgg tggcccttgc tggatttgaa
      181 ccagcgacct ggcgattatg agtcgctcgc tctcaccact gagctaaagg gccgagcgta
      241 ggataataac gttacgaaat caatgttgca agagttcaca aattcactga ttcaaaatta
      301 ttcttgtaaa aaatctttta acgtcaggca caaagagcct gtacatagat ttgtgtaatt
      361 gcctgatttt gatatgttca atccagcatc aaatgaaggt taatttatgg acgaaaaaca
      421 gttacaggct ctaatgacct ttatacgatt gacccaaata aaaaaagcca ccgttgcaac
      481 ttaagagtca ctaacggcag cttatgcgaa tagtgttgct catttgctca acgatgtcaa
      541 cacgttctat gctacatgct taattttctc tacacgtttc cggtttttaa acgcactatc
      601 cagaatcggg taaatcataa acaacgaggc attgaggatt tcgtcaactt cccgtcggca
      661 ggttgctagc gatggttttt gaatgcgccc gccgccccgg cataacatct tgcgaggtct
      721 tgcgacgcta tgatagtaag atgcaatggc gtgcttggaa gagccgtggg cgtagtagct
      781 gaggaggatg ccaaaggctt tcttgtcaat gtacatgacg gaatcgacga cctgagaaat
      841 caacattcca tcatcatcat tacacattgg ccttgtcata actcttcccg gctccacgct
      901 ctccatgaat ttagctatta cgctgctcat gcgcttttcc agacgacctg aataaaccca
      961 tgcgccccac agttcaagcc agccattcag ccactcatgc tgttctttgg tgaggtttag
     1021 ttctcttatg cccacgcgcc ttctccctgt acctgaatca atgtgagatt tccgcagaac
     1081 actgcgccgg tatcgatata catctggttg gcaaatttga gtggtttcac tgct
//
LOCUS       DBFIFM010000443         1125 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.453, whole genome shotgun sequence.
ACCESSION   DBFIFM010000443 DBFIFM010000000
VERSION     DBFIFM010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.453"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..>1125
                     /locus_tag="N7C74_005013"
     CDS             join(<1..281,281..>1125)
                     /locus_tag="N7C74_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1203 family transposase"
                     /protein_id="HGU2434676.1"
                     /translation="TRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTPETLR
                     VWVRQHERDTGGGDGGLTSAERQRLKELERENRELRRSNDILRQASAYFGEGGVRPPL
                     EKMMPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQHDDWLKREIQ
                     RVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTISRKAV
                     AAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSSMETT
                     FVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTERLKEAGLLASTGSTGDSYDNAMA
                     ESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLG"
     misc_feature    271..387
                     /locus_tag="N7C74_005013"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          264 a          267 c          348 g          246 t
ORIGIN      
        1 tactcgtttt tcccccgaag tccgtcagcg ggcgattcgt atggttctgg aaagtcagga
       61 tgaatatgac tcacagtggg cggcaatttg ttccattgcc ccaaagattg gctgtacgcc
      121 ggagactctg cgtgtctggg ttcgccagca tgagcgggat accgggggcg gtgatggtgg
      181 gctcaccagc gctgaacgtc agcgtctgaa agagctggaa cgtgaaaatc gtgaactgcg
      241 ccgcagtaac gatatccttc gccaggcttc cgcttatttt gcgaaggcgg agttcgaccg
      301 cctctggaaa aaatgatgcc actgctggat aagctgcgtg agcagtacgg ggtcggaccg
      361 gtatgcagcg aactgcatat tgccccgtca acgtattacc attgtcagca acagcgacat
      421 catccggata aacgcagtgc ccgtgcgcag cacgacgact ggctgaagag agagatacag
      481 cgcgtatacg atgaaaatca tcaggtgtac ggtgtgcgta aagtctggcg tcagttgtta
      541 cgggaaggaa tcagggtggc cagatgtaca gtggcacgtc tcatggcggt tatgggactt
      601 gccggtgttc tccggggtaa aaaggtccgt acgaccatca gccggaaagc cgttgccgca
      661 ggcgaccgcg taaaccgtca gttcgtggca gaacgacctg accagctgtg ggtggctgat
      721 tttacttacg tcagcacatg gcagggcttc gtctatgtgg cgtttatcat tgatgtgttt
      781 gccggataca tcgtggggtg gcgggtctca tcgtctatgg aaacgacatt cgtgctggat
      841 gcgctggagc aggcgttgtg ggcccgtcgt ccgtctggca ccatccatca cagcgataaa
      901 ggctctcagt atgtgtcact ggcctatacg gagcgactaa aagaagccgg attactggca
      961 tcaacaggga gtacaggcga ctcgtatgac aacgcgatgg ctgagagcat caatggtctt
     1021 tacaaagcgg aggtaataca ccgtaagagc tggaaaaacc gtgcagaagt ggaactggcc
     1081 acactaacgt gggtggactg gtataacaat cgacgattgc tggga
//
LOCUS       DBFIFM010000444         1108 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.454, whole genome shotgun sequence.
ACCESSION   DBFIFM010000444 DBFIFM010000000
VERSION     DBFIFM010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.454"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            48..1001
                     /gene="ompT"
                     /locus_tag="N7C74_005014"
     CDS             48..1001
                     /gene="ompT"
                     /locus_tag="N7C74_005014"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HGU2434677.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVSVNAGYYVTPNAKVYVEGTWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
BASE COUNT          354 a          228 c          237 g          289 t
ORIGIN      
        1 taaaaaatac atattcaatc attaaaacga ttgaatggag accttttatg cgggcgaaac
       61 ttctgggaat agtcctgaca acccctattg cgatcagctc ttttgcttct accgagactt
      121 tatcgtttac tcctgacaac ataaatgcgg acattagtct tggaactctg agcggaaaaa
      181 caaaagagcg tgtttatcta gccgaagaag gaggccgaaa agtcagtcaa ctcgactgga
      241 aattcaataa cgctgcaatt attaaaggtg caattaattg ggatttgatg ccccagatat
      301 ctatcggggc tgctggctgg acaactctcg gcagccgagg tggcaatatg gtcgatcagg
      361 actggatgga ttccagtaac cccggaacct ggacggatga aagtagacac cctgatacac
      421 aactcaatta tgccaacgaa tttgatctga atatcaaagg ctggctcctc aacgaaccca
      481 attaccgcct gggactcatg gccggatatc aggaaagccg ttatagcttt acagccagag
      541 gtggttccta tatctacagt tctgaggagg gattcagaga tgatatcggc tccttcccga
      601 atggagaaag agcaatcggc tacaaacaac gttttaaaat gccctacatt ggcttgactg
      661 gaagttatcg ttatgaagat tttgagctag gtggcacatt taaatacagc ggctgggtgg
      721 aagcatctga taacgatgag cactatgacc caggaaaaag aatcacttat cgcagtaaag
      781 tcaaagacca aaattactat tctgtttcag tcaatgcagg ttattacgta acacctaacg
      841 caaaagttta tgttgaaggc acatggaatc gggttacgaa taaaaaaggt aatacttcac
      901 tttatgatca caatgataac acttcagact acagcaaaaa tggtgcaggc atagaaaact
      961 ataacttcat cactactgct ggtcttaagt acacctttta acgaagttaa ccagattttc
     1021 tccccggtgg tattgtataa ccccggggaa tttgtttaca gttacaagta gacaatatcc
     1081 actaattttg taagcattta acctatgt
//
LOCUS       DBFIFM010000445         1101 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.455, whole genome shotgun sequence.
ACCESSION   DBFIFM010000445 DBFIFM010000000
VERSION     DBFIFM010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.455"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            721..789
                     /locus_tag="N7C74_005015"
                     /pseudo
     CDS             721..789
                     /locus_tag="N7C74_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
     gene            848..>1101
                     /locus_tag="N7C74_005016"
     CDS             848..>1101
                     /locus_tag="N7C74_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434678.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQRRNRYAIKKV"
BASE COUNT          357 a          229 c          256 g          259 t
ORIGIN      
        1 taaaccatgc agttatgatc cctgccgcat aaccttcatc agccacattt tcaaaaataa
       61 atttccttat atatcagaat catacttcgt agagttaata aatcaccaaa attcgtacca
      121 atagttcttg ataatgtcga actattggtt cattattatc atcgtcagca ggacgcatta
      181 ctcaccaggg cggtgaatat acaacgattc gaatatgaat ctacggcgct gacaaagcgc
      241 aataaccaaa gtgaactttg gggtgtggtg aagggttcat ggacgggaat atgtcgcacg
      301 taaagcggcg aggcctgcgg gactattgcc gaattgaagt aggccgaaac aggtcgaaat
      361 gggtctccca cctaccacac caccaaagtt catcaggagg tctatatgac acgcagaact
      421 cagttcaaag gcaattcacg ttctcgtcgt cgtgagcgtt taaaggcaaa ggcattagct
      481 aacggcgtac tggcccgcga agaagcaata agttcagaag tattacaccg ccctactcta
      541 agcagagcgc agattcaggc taaaggtact cacgaaacgc ctgagcgcat agaagacgct
      601 aagccaatta agttcatggc acaggacgtg atctggcaac agaaagaata cagacgcaat
      661 ctggagcgag cggccattgt gtacgcgaat gagtttggac ataagcaacc agaaactggt
      721 gtatgtcttc caaacgtagc catttacgcg gcaggctacc ggaaatcaaa acaactgacg
      781 gcgaggtgac ttgtgttggt cgccagaaaa tgaaattagg cagcaaacca cttatttgag
      841 gtgagatatg acaaaatcat ggagcgtacc ttttcctgaa tcagaaactg aacatgatgg
      901 aatgcctgtt ttctggagat tccaggcgac agttgaagaa gatgggataa aaatattcgc
      961 acttcaatat atagcttttc atcagacaga gcattatgca tggttggttc ctgcgcattg
     1021 gattgttaat tttaaaccag caccaaatca gtggttacag gaatggaaac aaaggagaaa
     1081 tagatatgca attaagaaag t
//
LOCUS       DBFIFM010000446         1094 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.456, whole genome shotgun sequence.
ACCESSION   DBFIFM010000446 DBFIFM010000000
VERSION     DBFIFM010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.456"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(51..422)
                     /locus_tag="N7C74_005017"
     CDS             complement(51..422)
                     /locus_tag="N7C74_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU2434679.1"
                     /translation="MARDIQMVLERWGAWAANNHEDVTWPSIAAGFKGLIPTKVKSRP
                     KCSDDDAMIICGCMARLNKNNQYLHDLLVDYYVGGMTFMALARKHRCSDGLIGKRLYK
                     AEGIIEGMLMALNVRLDMDMR"
     gene            complement(412..783)
                     /locus_tag="N7C74_005018"
     CDS             complement(412..783)
                     /locus_tag="N7C74_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU2434680.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVPGGRLGVKIYEIRGGNDGA"
     gene            complement(796..>1094)
                     /locus_tag="N7C74_005019"
     CDS             complement(796..>1094)
                     /locus_tag="N7C74_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU2434681.1"
                     /translation="SFMKRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQG
                     GMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT          298 a          306 c          209 g          281 t
ORIGIN      
        1 aacatgttaa ttataaacac agaaaacata accctcatca ctatatatcc ctaccgcata
       61 tccatatcta accggacatt cagagccata agcattcctt caataatacc ttccgcttta
      121 taaagccttt taccaataag cccatcagaa catctatgct tacgtgcaag agccataaat
      181 gtcattccac ctacgtaata atccaccaac aaatcgtgca aatactgatt attcttgttt
      241 aatcgtgcca tacaaccaca aattatcatg gcgtcatcat cagaacactt aggacgtgat
      301 ttcactttag tcgggattaa tcctttaaaa ccagcagcta tcgagggcca tgttacatct
      361 tcatgattat ttgctgccca tgccccccat cgctcaagaa ccatctggat atcacgcgcc
      421 atcgttacca cctctgattt cgtaaatctt cacgcccagc cgtccacctg gcacaggctg
      481 accgcgcaca atattgattt catcaaactg ctcgtcatca atgagcactt ccgcatgcgt
      541 cagcgcatcc agcggtgctt tcagaatgtt gtccaggtca cggcggcgct tatccggtgg
      601 ttctgcaata atttttattg ccagccgtcc ggacaggctt aatttcagcc gctgctggcg
      661 aacaataagc gccacagccc ggcgataacg ctcaccggct tttgatacaa aatatgtgct
      721 gccacgacgt cgccagtagg tgttcaccgt cggcgggtaa ggcaaaacaa actctatacg
      781 catcagtaac ctcttttacc cgagcacgcc ggttgcaaaa gcgtgatcaa gaaaacgaaa
      841 aattaaatca acctgggaac catgcttttc ttcgaacgcc agcggatccg catgaagctc
      901 gttgtgatgc tcccgacaca gcggtagcgt gaaaatatcg tgggattttg ttcccattcc
      961 accctgacca tgaccaatca gatgatgagg atcgtccgct ggcttaccac aacacgcaca
     1021 cggctgtgtc tttacccagc gtgtgtattt ctcatttacc cagcggcgac gtttaggtcg
     1081 tttcatgaag gatt
//
LOCUS       DBFIFM010000447         1087 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.457, whole genome shotgun sequence.
ACCESSION   DBFIFM010000447 DBFIFM010000000
VERSION     DBFIFM010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.457"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            10..255
                     /locus_tag="N7C74_005020"
     CDS             10..255
                     /locus_tag="N7C74_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU2434682.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
     gene            333..548
                     /locus_tag="N7C74_005021"
     CDS             333..548
                     /locus_tag="N7C74_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU2434683.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            553..1086
                     /locus_tag="N7C74_005022"
     CDS             553..1086
                     /locus_tag="N7C74_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU2434684.1"
                     /translation="MSRKLRYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
BASE COUNT          258 a          250 c          344 g          235 t
ORIGIN      
        1 gaaaatccca tgtcagaaat tacatccctg gtcactgctg aagcagtgaa ggaagtcctg
       61 cgctctgaag aagtccggag cgcactgaaa cagaaacttc gccataacct ggaagcgcgt
      121 cttgatgcag aagtggatgc cattctggat gaactgctgg gcgcaccggc agctccggag
      181 ccggaaggca tcgcgggtga ggggagtgct tcagatagcg gtgaccccac accggacagc
      241 gacatgatga tgtaagcatg cgtcagggac catcggtgtg tgccggtggt cttttttatt
      301 gttgtgagct tccggattgc gggagacggg gtatgtacca gatggaaaaa atcacaacag
      361 gtgtgtcata caccacgtca gcggtgggaa cgggctactg gttcctgcag ttgctggaca
      421 gggtttcccc gtctcagtgg gcggcaatag gcgtgctggg gagtctgctg tttgggctgc
      481 tgacatatct gacgaacctg tatttcaaga ttaaagaaga ccggcgtaag gcggcacggg
      541 gagagtaagc tgatgagcag gaaactccgc tatggtttat cggctgccgt tctggcgctg
      601 attgccgcag gtgcttctgc gcctgaaatc ctcgaccagt ttctggatga aaaggaaggt
      661 aaccacacca cagcataccg tgatggtgcg ggtatctgga ccatctgccg tggagccacc
      721 cgggtggatg gtaagcctgt tattcctggc atgaagctgt cgaaggaaaa atgcgaccgg
      781 gttaacgcca ttgagcgtga taaggcgctg gcatgggtgg agaaaaacat taaagtgccg
      841 ctgaccgaac cccagaaagc ggggatcgcg tcattctgtc cgtacaacat tggtcccggt
      901 aagtgtttcc cgtcgacgtt ttacagacga attaatgcag gtgatcgaaa aggtgcctgc
      961 gaagctattc gctggtggat taaggacggt ggcagagact gccgtattcg ttcaaacaac
     1021 tgttacggtc aggtatcccg tcgtgaccag gagagcgcgc tggcgtgctg gggaatcgac
     1081 agataag
//
LOCUS       DBFIFM010000448         1079 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.458, whole genome shotgun sequence.
ACCESSION   DBFIFM010000448 DBFIFM010000000
VERSION     DBFIFM010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.458"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            379..963
                     /locus_tag="N7C74_005023"
     CDS             379..963
                     /locus_tag="N7C74_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGU2434685.1"
                     /translation="MDKRAKNQIVDSDIARLLLKLRKSRNLTVTELAQRSGVSQAMIS
                     KVERGTSSPSATILSRLANALNITLSKLFAELEMQQNSLVLLADQQQHWIDEETGITR
                     WSLSPAGACPELIKVEIPPMGQLTIPASANEHLSGQTLWMLSGSLDFRVNHQTHHLQA
                     GDCLALTFASEYQMRNPDSGQSCSYIVAFSQKNP"
     gene            complement(988..>1079)
                     /locus_tag="N7C74_005024"
     CDS             complement(988..>1079)
                     /locus_tag="N7C74_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU2434686.1"
                     /translation="QHMGFKLSGRSERDNQGRPYPLLHLYNAM"
BASE COUNT          319 a          203 c          222 g          335 t
ORIGIN      
        1 tttatttatt ctttatgggc agcttgccaa gtgttgtatt attgttgtgg tttaatatgc
       61 tggaaatttt ctttttgctg atatttttat ctgtctatag ttattcattt atacgcatat
      121 tagcaaaaaa aatattattt taggtatttc tacagtacat ttgacacgat gattaacctc
      181 tatgtagcta ggtttttgta aagaaactgt gcgtcactgt atccaggact gttatgtgcc
      241 aaataaggaa gttgaagtta ccagctctta ctctaaagct ataacgtata gaaaaaatgc
      301 atttgagacg tgtaaaatca tttatttaat cgcaatttat gtaatggcat aatcaggtcg
      361 gtgaaagagg ggagaagaat ggataaaaga gctaaaaatc agatagtcga tagcgatatt
      421 gcacgtttgt tactcaagct cagaaagtct cgaaacttga ctgttacaga gcttgcccaa
      481 cgatctggtg tcagtcaggc gatgatcagc aaagttgaac gaggcacttc aagccccagt
      541 gcaacaatac ttagtcggtt agctaatgca ctgaatatta cgctatcaaa gctttttgca
      601 gagcttgaga tgcaacagaa ttcacttgta cttttggctg accagcaaca acattggatt
      661 gatgaagaaa caggcattac ccgctggtct ttgtccccag cgggtgcatg tccggaacta
      721 ataaaagttg aaatcccccc tatggggcaa ttgactatcc ctgcctctgc aaatgagcat
      781 ctttctggac aaactctatg gatgttaagc ggcagccttg attttcgagt caatcatcag
      841 actcatcatt tacaagccgg tgattgtctt gctcttacct ttgccagcga gtaccaaatg
      901 agaaacccag atagcggcca gagttgttct tacattgtcg ctttcagcca aaaaaatcca
      961 tgatgtaatt ctcctaaaat tatcatgtca cattgcgtta taaagatgta atagagggta
     1021 gggtctgcct tgattgtccc gctcggaacg tcctgaaagt ttaaagccca tatgttgat
//
LOCUS       DBFIFM010000449         1070 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.459, whole genome shotgun sequence.
ACCESSION   DBFIFM010000449 DBFIFM010000000
VERSION     DBFIFM010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.459"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            52..1011
                     /locus_tag="N7C74_005025"
     CDS             52..1011
                     /locus_tag="N7C74_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HGU2434687.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSEFKTGDDYSFINGAITFFF"
BASE COUNT          370 a          201 c          209 g          290 t
ORIGIN      
        1 tcatgtatgt aaaatacgcg caccattcaa aaaaaaggaa gacaataaca tatgaaaaaa
       61 agtgtcatcg ctggcgtctt tattgctctg tcatttacca cgtgttcagc tatcgcgaac
      121 agccttgcat tatcattagc aaatgatgat gcagggaagt ttcaaccaat acttaatgat
      181 atttatggca ataaacatga aaacagagat gattactcac aaggcttatt tctgggatat
      241 agccacgata tctcagactc gagccaatta tctctccata ttgcgcaaga tatttactct
      301 ccatcaggca gtaataaaag acacaacaca gctgtaactg gagacagagc ttttagtgca
      361 tacactcaca ctggtattga atggaactcc cttgcgaatg actggattcg ctatcgatta
      421 ggtactgaca taggtgttgt tggccccgac gcaggcggtc agaaagtaca aaataaagct
      481 catgagatta ttggggcaga aaaatatcat gcatgggatg atcaaataga gaatcgctac
      541 ggttatactg taaaagggat gctatccatg acaccaagta tggatatttt aggtgctaat
      601 gttggattat accctgaagt ttctgctgtt actggaaact tatttcaata tgtagcatat
      661 ggcgcaacca ttgccattgg taatgataaa accttcaatt cggataatgg ctttggtctg
      721 ctggctcccc gtggtttaat gcatatgtcc gatacaagcg gattcaaata caagattttt
      781 gcaggtatgg aaagacgaga tgtcaatcgc aactatactc tcgaaggaaa aacaatacag
      841 acgaaacaaa caacagtatc gctaaacaaa actgttgatg aatatcaagt tggcgcaaca
      901 attgggtatg cacctgtagc cttcacacta gcatttaata aagtaacatc agaattcaag
      961 acaggggatg actattcatt tataaatgga gcaatcacct tcttttttta actgaattga
     1021 attcaatcaa aataacataa gtccaacaaa aacataaagt gcgaaatgaa
//
LOCUS       DBFIFM010000450         1056 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.460, whole genome shotgun sequence.
ACCESSION   DBFIFM010000450 DBFIFM010000000
VERSION     DBFIFM010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.460"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..>1056
                     /locus_tag="N7C74_005026"
     CDS             <1..>1056
                     /locus_tag="N7C74_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HGU2434688.1"
                     /translation="DSRGQLIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDA
                     WGKAICTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRY
                     HHDLTGKLIRSEDEGLVTHWHYDEADRLTHRTVKGETAERWQYDERGWLTDISHISEG
                     HRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVE
                     WLTYGSGWLAGMKLGDTPLVDFTRDRLHRKTLRRFGRYELTTAYTPAGQLQSQHLNSL
                     QYDRDYTWNDNGELIRISSPRQTRSYSYSDSGRLTGVHTTAANLDIRIPYATDPAGNR
                     LPDPELHPDSTLSMWPD"
BASE COUNT          254 a          340 c          312 g          150 t
ORIGIN      
        1 cgacagccgt ggacagttaa ttgccgtgaa agacacgcag ggccatgaaa cgcggtatga
       61 atacaacgcc gccggtgacc tgaccaccgt cattgccccg gacggcagca gaaacgggac
      121 acagtacgat gcgtggggaa aagccatctg taccacgcag ggcggtctga cgcgcagtat
      181 ggaatacgat gctgccggac gggtcatccg cctgaccagt gaaaacggca gccacaccac
      241 cttccgttac gatgtactcg accggctgat acaggaaacc ggctttgacg gccgcacaca
      301 gcgttatcac cacgacctga ccggcaaact tatccgcagc gaggatgagg ggctggtcac
      361 ccactggcac tatgacgaag cagaccgcct cacgcaccgc accgtgaagg gtgaaaccgc
      421 agagcgctgg cagtatgacg aacgcggctg gctgacagac atcagccata tcagcgaagg
      481 gcaccgggtg acggtgcatt acgggtatga tgagaaaggc cggctgaccg gtgagcgtca
      541 gacggtgcat cacccgcaga cggaagcact gctctggcag catgagacca gacacgctta
      601 caacgcgcag gggctggcga accgctgtat accggacagc ctgcccgccg tggaatggct
      661 gacctatggc agcggctggc tggcaggcat gaagctcggc gacacaccgc tggtggattt
      721 cacccgcgac cgcctgcacc ggaaaacgct gcgcagattc ggccgttatg aactcaccac
      781 cgcttatacc cctgccgggc agttacagag ccagcacctg aacagcctgc agtatgaccg
      841 cgattacacc tggaacgaca acggcgaact catccgcatc agcagcccgc gccagacccg
      901 gagttacagc tacagcgact ccggcaggct gaccggcgtt cacaccaccg cagcgaatct
      961 ggatatccgc atcccgtatg ccacggaccc ggcaggtaac cgcctgcccg acccggagct
     1021 gcacccggac agcaccctca gcatgtggcc ggataa
//
LOCUS       DBFIFM010000451         1052 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.461, whole genome shotgun sequence.
ACCESSION   DBFIFM010000451 DBFIFM010000000
VERSION     DBFIFM010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.461"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            115..1035
                     /locus_tag="N7C74_005027"
     CDS             115..1035
                     /locus_tag="N7C74_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU2434689.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTSLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPDAALEAGLTEIDLFEATEYYRSPRGYAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPIMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
BASE COUNT          298 a          214 c          252 g          288 t
ORIGIN      
        1 atgataacca atacaagtca tactattacg ccgttctcga tatgaacaca aatgccagtt
       61 gcggataata gaaatgatat ttaaatctgt ttaggcaaat ccccggagct tgaaatgaaa
      121 actgtttcga taaaacacac ttactgggat atagctgctg atatctattt ccctgacgat
      181 tttaatagtg aaaaaaagta tcctgccatt atcagcgccc atccaattgg aagctgcaaa
      241 gaacagacat cgggtagtgt gtatggtgcc gccctggcaa aagctggctt tatcgtcatt
      301 gcttttgatg ctagttttca gggaagcagc ggtggggaac cgcgttatct ggaagatcca
      361 acaatgcgag taaaagactt cagcattgtc gttgattacc ttaccagctt accttacgtt
      421 gatgccgggc gtattggtgt actgggtatt tgtggtggag gtggatatgc cattaatgcc
      481 gcaatgacag aacgccgaat taaagctatt ggtactgtca cgggtgcaaa ctacgggcgt
      541 cttatgcgtg agggatttac agcgtttaat cctatcggag cacttgaagc aatggcgcaa
      601 caaagaacag acgaagctaa cggcgcaaaa cttcgcgtag atgatttact cccctcttct
      661 ccggatgcag ctcttgaagc gggactaaca gaaatagacc tttttgaagc cacagaatat
      721 tatcgttcgc cacgaggcta tgcacctaat ggggtaaatc gttcgctatt ctcacatcag
      781 actgttgccg tgggttggga tgcatttcat cttgcagaag tgctgttaac tcaaccaata
      841 atggttgtgg tgggcgatag agtgggcgca tttggtgcct accgtgacgg ctgcgaaatt
      901 attggccgcg ctgcttcaaa acataaagag ctggtcgtgg ttgaaggtta ttcacattac
      961 gacctgtatg ataagccaga acctgtgaaa caggcactcg ataaattaat tcctttttat
     1021 aaaacacatt tgtaataatt ttacagggag at
//
LOCUS       DBFIFM010000452         1050 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.462, whole genome shotgun sequence.
ACCESSION   DBFIFM010000452 DBFIFM010000000
VERSION     DBFIFM010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.462"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..917
                     /locus_tag="N7C74_005028"
     CDS             <1..917
                     /locus_tag="N7C74_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HGU2434690.1"
                     /translation="GEASLSLVITAKGNHTRNFSNLRELESLEGHYWDEESSRGYIAP
                     YNAQVNLAEKVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQHSRNIAFVDNPEL
                     VNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIKYYADDGEIFESPVISAFDLLYS
                     EYDKSLERLNSRLNSNDSHFKSEQIVACLLRDILSQDSYRSMMFHSQIALNQLVLLER
                     GDFTHREQLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQAERDELKNSILKK
                     CGLPLLRLRTIDSDIEGKLGAFLSGLTG"
BASE COUNT          293 a          197 c          269 g          291 t
ORIGIN      
        1 ccggtgaagc ctcattatct ctggtcatca cggcaaaagg aaaccatacc cgtaattttt
       61 caaatctcag ggagcttgaa tctctggaag gtcattactg ggatgaagaa agtagcaggg
      121 gatacattgc cccatataat gcacaggtta atctggctga aaaagtgctg cctgctgatt
      181 ttgtgaaatc gacggtgcat aaatttcagg gacgggagtg cgatgaaatt gtgttttcca
      241 ctgtgctgga taagaagcgc agcagccagc acagtcggaa catcgcgttt gttgataatc
      301 ctgaactggt gaatgtcgct gtttcaaggg cccgaaataa atttacgctg gtgactggta
      361 atgatgtatt tgaacgccac gctggacata ttgcggcgct gattcgttac atcaaatact
      421 acgctgatga cggagagatt ttcgagagcc cggtcatatc agcatttgat ttgctgtaca
      481 gtgagtatga caaatcactg gaacgactga atagtagatt gaattcaaat gattcacact
      541 ttaagtcaga acagattgtg gcctgcctgt tgcgggatat cctgtcgcag gatagttatc
      601 ggagtatgat gtttcattca caaatagcgc tgaatcagtt ggtgttgctg gagcgggggg
      661 attttacaca ccgtgaacag ttattcatgc gtaaccgggc cagttgtgac tttgttgttt
      721 actataaggt cgggaagact ccattagggg ttattgaagt ggatggaggt tatcatctga
      781 ccagtgttca ggctgaacgt gatgaactta aaaacagtat actgaagaaa tgcggcctgc
      841 cattgctaag attacgtact atagacagtg atatagaagg gaagttggga gcgtttctgt
      901 caggcctgac aggctaaaat ctgaataaaa aatattgcag cgggaggtct caaacctgat
      961 ttggtcatgt gccacacaca attgtacgaa ccccatggac acaggcttct ttgttttggt
     1021 tacatcaacg tacgtattcc tgatacatat
//
LOCUS       DBFIFM010000453         1049 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.463, whole genome shotgun sequence.
ACCESSION   DBFIFM010000453 DBFIFM010000000
VERSION     DBFIFM010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.463"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            37..>1049
                     /locus_tag="N7C74_005029"
     CDS             37..>1049
                     /locus_tag="N7C74_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU2434691.1"
                     /translation="MKKAIAYMRFSSPGQMSGDSLNRQRRLIAEWLKVNSDYYLDTIT
                     YEDLGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLK
                     LILNHGIDVITLCDNTVYNIDSLNEPYSLIKAILIAQRANEESEIKSSRVKLSWKKKR
                     QDALESGTIMTASCPRWLSLDDKRTAFVPDPDRVKTIELIFKLRMERRSLNAIAKYLN
                     DHAVKNFSGKESAWGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDL
                     FYAVQEIRLAPFGISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMR
                     RLHR"
BASE COUNT          350 a          160 c          236 g          303 t
ORIGIN      
        1 taaggtttat aaaagcataa gttagcaggt gtcaccatga aaaaagccat agcatatatg
       61 cgattttcat caccaggtca gatgtctggc gactcattaa accgacagag aagacttatt
      121 gctgaatggt taaaggtaaa tagtgattat tatcttgata ccataacata tgaagattta
      181 ggattaagtg cattcaaagg aaagcatgca caatcaggag ctttttcgga atttttagat
      241 gctatagagc atggttatat attgccagga actacattgt tagttgaaag tctggacaga
      301 ctttcaagag aaaaagtcgg tgaagcgatt gaacgtctga aattgatttt gaatcacggt
      361 attgatgtta taactctttg cgacaataca gtctataata ttgactcttt gaatgagcca
      421 tattcattaa taaaagccat acttatagca caaagggcaa atgaagaaag cgagataaag
      481 tcaagtcggg ttaaattatc atggaagaaa aaacggcagg atgcactgga atcaggtacg
      541 attatgacgg cgtcttgtcc gagatggctc tccttagatg acaaaagaac ggcttttgtt
      601 ccagaccccg acagggtgaa aactattgag ctaattttta aactcaggat ggaaaggcgc
      661 tcattgaatg caatagccaa gtatttaaat gatcatgctg taaagaattt ctcaggaaaa
      721 gaaagtgcat ggggaccttc tgtaattgaa aaattattag cgaataaagc tctgataggt
      781 atatgcgtac cttcatatcg tgcaagaggg aaagggataa gtgaaatcgc tggctattat
      841 cccagagtca tatcagatga tttgttttac gctgtacagg aaattcggtt ggcacctttt
      901 ggtattagca atagtagcaa gaatcctatg ctaataaatc tacttcgaac agttatgaag
      961 tgtgaggctt gtggtaatac catgattgtt catgcggtat ctggaagttt gcatggctat
     1021 tatgtttgtc cgatgagaag attacatcg
//
LOCUS       DBFIFM010000454         1045 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.464, whole genome shotgun sequence.
ACCESSION   DBFIFM010000454 DBFIFM010000000
VERSION     DBFIFM010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.464"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..450
                     /gene="escC"
                     /locus_tag="N7C74_005030"
     CDS             <1..450
                     /gene="escC"
                     /locus_tag="N7C74_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HGU2434692.1"
                     /translation="NVTFYTKVSGEKVASLESITSGTLLRVTPRILDDSSNSLTGKRR
                     ERVRLLLDIQDGNQSTNQSNAQDASSTLPEVQNSEMTTEATLSAGESLLLGGFIQDKE
                     SSSKDGIPLLSDIPVIGSLFSSTVKQKHSVVRLFLIKATPIKSASSE"
     gene            464..919
                     /gene="cesD"
                     /locus_tag="N7C74_005031"
     CDS             464..919
                     /gene="cesD"
                     /locus_tag="N7C74_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HGU2434693.1"
                     /translation="MSRKFSSLEDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNKKMAKKAFKACLMWCNEKEKYTTYKENIKKLLGNTE"
BASE COUNT          353 a          197 c          206 g          289 t
ORIGIN      
        1 aacgtaacgt tttatacaaa agtctccgga gaaaaggtgg cgtctctgga aagtattaca
       61 agcggaacat tgctgagagt gacaccacgt atacttgatg acagctcgaa tagccttacc
      121 ggaaaacgtc gggaacgagt tcgtttgtta cttgatattc aggatggcaa tcaatcgact
      181 aaccaaagca atgctcagga tgccagcagc accttacctg aagtacaaaa ttcagaaatg
      241 acaacagaag cgacattaag tgcaggagaa agtttattac ttggcggatt tatccaagat
      301 aaagaaagtt caagtaaaga tggtataccg ctgttaagcg acattcctgt tattggtagt
      361 ttattctcca gtacggtaaa acaaaaacat agtgttgttc gactcttttt aattaaagca
      421 accccgataa aatctgcatc tagcgaataa taaatgaggc tttatgagca ggaaatttag
      481 ctctctagag gatatttatg atttctacca ggatggtggc acattagcgt cattaacaaa
      541 tctgacacaa caagatctca atgaccttca ttcttatgcc tatacagcat atcaatctgg
      601 tgatgttata accgcaagaa atctattcca tttgctcaca tatctggaac actggaatta
      661 tgactacacc ttatctctgg gcttatgtca tcagcgttta tcaaatcatg aagatgcaca
      721 actgtgtttc gcacgctgtg caactttagt tatgcaagat cccagggcat cttattattc
      781 tggaattagc tacttactcg tcggaaataa gaaaatggcc aagaaagcct ttaaggcttg
      841 tttaatgtgg tgtaatgaaa aagaaaaata cactacatat aaagaaaata ttaaaaaatt
      901 gttaggtaat acagagtaaa aactaccatc agctatacca atccccaggc tgactgggga
      961 ttttctatcg gaaataataa agctgctata gtgaagtgct cccaaagttg agcaccgacc
     1021 actggtagat ttatctacag gtgtg
//
LOCUS       DBFIFM010000455         1043 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.465, whole genome shotgun sequence.
ACCESSION   DBFIFM010000455 DBFIFM010000000
VERSION     DBFIFM010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.465"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..388
                     /gene="espM2"
                     /locus_tag="N7C74_005032"
     CDS             <1..388
                     /gene="espM2"
                     /locus_tag="N7C74_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM2"
                     /protein_id="HGU2434694.1"
                     /translation="EWIKDERIVYPSRVINQEIDNYCFQKNAKISTEERQRVFFLVSQ
                     ENQLTLDVKAAQSSINHVIMGSASFGKKMDALCDGMSRDVKNRTSDTIANLLADKFYQ
                     KHIDSDIDIVKLRNDIPDYLMRAIQG"
     gene            622..774
                     /locus_tag="N7C74_005033"
                     /pseudo
     CDS             622..774
                     /locus_tag="N7C74_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(890..>1043)
                     /locus_tag="N7C74_005034"
     CDS             complement(890..>1043)
                     /locus_tag="N7C74_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGU2434695.1"
                     /translation="KRYDDVEVIVKATSNDGLSVTRTADKDSAKTFVQETLKDTWKSA
                     DEWFVH"
BASE COUNT          326 a          202 c          243 g          272 t
ORIGIN      
        1 tgagtggata aaagatgaac ggatagtata tccctcaagg gtgatcaacc aggagattga
       61 taattactgt tttcaaaaaa atgcaaaaat ttccactgaa gagaggcaaa gagtcttttt
      121 tcttgtgagc caggagaatc aactaaccct tgatgttaag gcagcacaaa gttctattaa
      181 tcatgtaata atggggagtg cttcttttgg caagaaaatg gatgctcttt gtgatggtat
      241 gagccgggat gtaaaaaatc gtacatcaga taccatagca aacttgcttg cggacaagtt
      301 ttaccagaaa catatcgatt cggatattga tattgtaaaa ctacgaaatg atatcccaga
      361 ttatttgatg cgtgctatac agggatgagg ctagaggaga ggactgcttc agcatcatcg
      421 gcttcggacc agtaaaagag acgcagaaaa tgcacaacgg caccacacgc catgcatgga
      481 ttcagattac tcatggctca ccagtacagc tataaatcgt aaagaaaact acagtacgtt
      541 atacacagaa acgtaccgtg attcattatt tattgtacag gttcatcgca gcgacaatat
      601 tttgtgatgt ttgcgtggtg cgccccactt caggatgctg cagatctgga aattgcaacg
      661 gaggaagaaa cctcattgct ggaagcctag aaaaagtatc gggtgttgct gaaccgtgtt
      721 gatacatcaa ctgcacctga tattgagtgg ccggaagaac cagacacaat gtaagcgaaa
      781 aagaaaaacc gcagacacga cgtatgcagg acgtgctgcg gttggctggt gaactttcga
      841 tagtgcgagt attgaatgat ttccagccgt taccgatttt acgtgttaat tagtgaacaa
      901 accactcgtc agcagacttc caggtatctt tcagagtctc ctgaacaaaa gttttagctg
      961 aatctttatc ggcggtgcgc gtaacagaaa ggccatcgtt gctggtggct tttacgatca
     1021 cctctacatc gtcataacgt tta
//
LOCUS       DBFIFM010000456         1038 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.466, whole genome shotgun sequence.
ACCESSION   DBFIFM010000456 DBFIFM010000000
VERSION     DBFIFM010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.466"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            1..703
                     /locus_tag="N7C74_005035"
                     /pseudo
     CDS             1..703
                     /locus_tag="N7C74_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344697.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system needle length
                     determinant, SpaN/EivJ family"
     gene            663..>1038
                     /locus_tag="N7C74_005036"
     CDS             663..>1038
                     /locus_tag="N7C74_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
                     /protein_id="HGU2434696.1"
                     /translation="MKANSKTIRRMKVNVRSEESKSKHSTFETTFQNWKENGEDVALL
                     MPEFSAKWLPIAEESGSWSGWVLLREIFPLISAELAGMALMPETERLIGEWLSLSSSP
                     LNLKYPELKYNRLCVGKVFDGVL"
BASE COUNT          378 a          185 c          220 g          255 t
ORIGIN      
        1 ttctacgttg gggaaaaaga atgggcacat gaagtctatt gacaacaaat cgtctctcta
       61 ggttgacagg aatccatctc tttctttgca aacaaaagga gccgagcaat tttctacaaa
      121 gaaacttctc aatatgactt cacgagatca gggtattaat tctgaactat ctaatcgaac
      181 gatacaattt aaggaaaaaa tccataatgg tatccataca gaatacatca cagatcaaaa
      241 acacagtaat aataaagatc gtgaaaaaaa ataccgcgat ggagacaaaa taaatggacc
      301 gcaggcccat tcattagaca ttactaacga aagaaggttt gctgataaca gaaccatgtt
      361 tactcaacat atcgaaaagc aaaggaatgt taatacgctc aatcagaatg atataaataa
      421 ttctgctaat aatgccaatg tgcgcgaaaa tgaattgaca tatcagtttc agcgttgggg
      481 acaaaatcat acggtccgga ttctggaaag tagtgaaggg atacgactaa aaccatcaga
      541 cacacttgtc tccgacagac tccatgaagc ccaacacaat gatgttacag cacaacgttg
      601 ggtattgact gagcaagatg aacgacaagg acaacgacat cagcctcatg aggagcaaga
      661 aaatgaaggc aaattcgaaa acgatcagaa ggatgaaagt taatgttcgg tctgaggaaa
      721 gtaaatcgaa acactctacg tttgaaacca cttttcaaaa ttggaaagaa aatggcgaag
      781 atgttgcatt attaatgccc gaatttagtg ctaaatggct accgatagca gaagagagtg
      841 gcagctggtc tggatgggtg ttgcttcgag aaatatttcc cttaatttct gccgagctcg
      901 ctgggatggc tttaatgcca gaaacggaaa ggttaatagg ggaatggctc agtttatcca
      961 gttccccgtt aaatctaaag tatcctgaac taaaatataa tcgtttgtgc gtaggtaaag
     1021 tatttgatgg agtattga
//
LOCUS       DBFIFM010000457         1037 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.467, whole genome shotgun sequence.
ACCESSION   DBFIFM010000457 DBFIFM010000000
VERSION     DBFIFM010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.467"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            63..434
                     /locus_tag="N7C74_005037"
     CDS             63..434
                     /locus_tag="N7C74_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434697.1"
                     /translation="MQKKEIRRLRLKEWFKDKTLPPKEKSYLSQLMSGRASFGEKAAR
                     RIEQTYGMPEGYLDAEYAEQPGGSPPHAGLTSNQLELLQIFSAFPEDEQRQIISELKQ
                     KKESMEDLIARWIAAQKCRRA"
     gene            455..646
                     /locus_tag="N7C74_005038"
     CDS             455..646
                     /locus_tag="N7C74_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HGU2434698.1"
                     /translation="MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDE
                     VERRMAERFAKMRKERSKQ"
     gene            648..926
                     /locus_tag="N7C74_005039"
     CDS             648..926
                     /locus_tag="N7C74_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU2434699.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVEVVNVIHARRQFP"
BASE COUNT          332 a          219 c          235 g          251 t
ORIGIN      
        1 tgtttaataa accattaagt caagtatttg cttgtttagt gattactaaa gataattacc
       61 acatgcaaaa aaaagaaatt cgccgtttac gtctcaagga gtggtttaaa gataaaactc
      121 tgccacccaa agagaagagc tacctatctc aactaatgag tgggagagcc tcgtttggag
      181 aaaaggctgc cagaagaata gagcaaacat acgggatgcc ggaagggtat ctggatgcgg
      241 aatacgcaga acaaccgggg ggttctccac cacatgcagg gttaacgtct aatcaactgg
      301 aattattgca gattttttca gccttccctg aggatgagca acgccagata atcagcgagt
      361 taaagcagaa aaaagaatca atggaagatc tcatagcgag atggattgcg gcgcaaaaat
      421 gccgccgcgc ctgagttata aaaccggagg aaacatgaat agagcccttt caccaatggt
      481 ttctgaattt gaaaccattg aacaagaaaa cagttacaac gaatggctgc gtgcgaaagt
      541 agcaacgagc cttgcagatc cgcgcccagc aattccccat gacgaagttg agcgcagaat
      601 ggcagaacgc tttgctaaga tgcgcaagga acggagcaag cagtaaaatg ttacccgtgt
      661 tatggcttga aagcgcagat accgacctag atgatataac tagttatatt gctcgtttcg
      721 acatagatgc ggctgaacgc ttatggcagc gattaagggg ttgtgtgctg ccgttatccg
      781 aacatccgta tttataccca ccaagcgaca gagtacctgg cttgcgtgag attgtagccc
      841 accctaacta tataattcta taccgcgtaa caacatcaag cgttgaagta gtaaacgtga
      901 tccacgcaag acgccagttt ccctaacttt cactaccaat agaaacataa caaccgcaac
      961 gactttatca aaagcgttgt gtttgttatg tcccgcggtt tagtttttac ttgacttaag
     1021 tttaatgttt attaaac
//
LOCUS       DBFIFM010000458         1035 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.468, whole genome shotgun sequence.
ACCESSION   DBFIFM010000458 DBFIFM010000000
VERSION     DBFIFM010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.468"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..409)
                     /gene="sctT"
                     /locus_tag="N7C74_005040"
     CDS             complement(<1..409)
                     /gene="sctT"
                     /locus_tag="N7C74_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HGU2434700.1"
                     /translation="MGEVILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELSTLNEIDIALLAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAV"
     gene            complement(411..671)
                     /locus_tag="N7C74_005041"
     CDS             complement(411..671)
                     /locus_tag="N7C74_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HGU2434701.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(682..>1035)
                     /locus_tag="N7C74_005042"
     CDS             complement(682..>1035)
                     /locus_tag="N7C74_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HGU2434702.1"
                     /translation="KYSEPELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALS
                     EIKSAFIIGFYIYLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTML
                     SKGLILQYFDLSINP"
BASE COUNT          361 a          227 c          166 g          281 t
ORIGIN      
        1 aaaccgctgc agagaaaagg ttaaataatc ttgctaattc cgaggtatcg acacctgtag
       61 ccggatccaa agtactactt aacgttgcac cgcgttggtt atcgataaag ctacctatag
      121 caaggaaaat ccaaaatgga gatgctaata agcaagcaat gaacagccct atgataattt
      181 ctcttgcagc taacaaagcg atatcgattt catttaaggt cgataattca tagtggtaat
      241 gctgaactaa tgcacatgat accactatta ttattggatg acggactact gttggtatat
      301 tcccactggc gaaaaatggt aacaaataga aagttggtgc caatcggcaa aaaccgagcg
      361 ctgtagcagc cagtagacta tgcaactgat agagtatcac ctcccccatg ctatcctctc
      421 gcaaatgcta aattcagcat ttcaatacca aaagaatata atttttcacc ataccatccg
      481 ctcattaaaa aaaagcaaat acttacgcac aataatttca cgccaaatgg caacgtctgt
      541 tcttgcaact gcgttaccgt ttgaaaaagc cctaccaata aaccaacaaa tgtcgctact
      601 gcaataggtc ctgcagacat aactaaaatc agatacagcg ccctattccc tgcaaaaaca
      661 atgtcatcca tatttttttc ctcagggatt aatggatagg tcaaaatatt gtaatatgag
      721 tccttttgat aacattgtcc aaccgtccat cgcgacaaac agaattaatt ttatcggtgt
      781 agatatggtt accggactca tcatcatcat acctaacgtt aacagaacgc tggatataac
      841 cagatccact acaacaaagg gtaaatagat ataaaaacct ataataaatg cactttttat
      901 ctcacttaac gcatatgccg ggagtaacga gaaaattgaa atattatcat catcgattat
      961 ctcttcattg tcttcagaac tattcacctt ctgaatcttt tcaaaaaagc taaccaactc
     1021 cggttcagaa tattt
//
LOCUS       DBFIFM010000459         1029 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.469, whole genome shotgun sequence.
ACCESSION   DBFIFM010000459 DBFIFM010000000
VERSION     DBFIFM010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.469"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..482
                     /gene="lpfA1"
                     /locus_tag="N7C74_005043"
     CDS             <1..482
                     /gene="lpfA1"
                     /locus_tag="N7C74_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA1"
                     /protein_id="HGU2434703.1"
                     /translation="GTVFAAESGDGTVKFTGEIVDSPCVLSVDSQNQEVVLGQVQKSV
                     FAAVGDKSPAKPFEIKLEDCDTTTMKKANVSFSGVGDADKSDLISVSTEAGAAKGVGI
                     GIYDNSNTLVALNGGKASVDLSKGQTVLYFTANYVSTLATVTTGYGNAQVDFNLSYE"
     gene            547..>1029
                     /locus_tag="N7C74_005044"
     CDS             547..>1029
                     /locus_tag="N7C74_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312457.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU2434704.1"
                     /translation="MKSKFVALALSLFLSQSVLAGGVGLSSTRVIYDGSKKEASLTVQ
                     NKSKTEEFLIQSWVDDAAGSKKTPFIITPPLFKLDPEKNNILRIVNITPGLPQDRESV
                     YWVNVKAIPSKSDDSENKNVLQIAVRTRIKLFYRPAGLKGDVKTAPNELRFTRNGNQL
                     R"
BASE COUNT          277 a          225 c          253 g          274 t
ORIGIN      
        1 ctggcactgt attcgcagca gagtctggtg acggcacggt taaatttaca ggcgagatcg
       61 tggattcacc ttgcgtactg tccgttgact ctcagaacca ggaagttgtg ttgggtcagg
      121 tacaaaaatc tgtatttgct gcggttggtg acaaatcccc ggcaaaacct ttcgaaatca
      181 aactggaaga ttgcgatacc accacgatga aaaaagccaa tgtgagcttt agtggtgttg
      241 gtgatgcaga taaatctgat ctgatttctg ttagcactga agcgggcgcg gccaaaggtg
      301 ttggtatcgg tatttatgat aacagcaata ccctggtcgc tcttaacggt ggtaaagcaa
      361 gtgttgatct gagtaaaggc cagacggttc tctactttac cgctaactac gtttctactc
      421 tggcaacggt aacgaccggt tacggcaatg cgcaggtcga tttcaacctg tcttacgagt
      481 aatcgtccgt tttacaaatg gcggcgtaat tgccgccatt agtcgtcaaa gttcatggat
      541 cgaatgatga aaagtaaatt tgttgcgttg gctctctctc tgttcctttc acagtcagtt
      601 ctggcgggtg gcgtcggact tagctctact cgcgtaattt atgatggcag taagaaagaa
      661 gcctcgctga ccgtacagaa taaaagtaag acggaagagt ttcttatcca atcctgggtg
      721 gatgatgccg cgggcagcaa aaaaacaccg tttattatta cgcccccact attcaagctt
      781 gatccagaaa agaacaatat tttgcgtatt gtgaatatta cgcccggcct gcctcaggat
      841 cgcgaatcgg tctactgggt taatgtgaaa gccattccat ctaaaagtga cgacagcgaa
      901 aacaaaaatg ttttgcagat tgccgttcgc acccgaatca aactttttta tcgcccggca
      961 ggtttaaagg gtgatgtaaa aaccgcgcca aatgaactgc gatttacgcg taacggtaat
     1021 cagctccgc
//
LOCUS       DBFIFM010000460         1025 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.470, whole genome shotgun sequence.
ACCESSION   DBFIFM010000460 DBFIFM010000000
VERSION     DBFIFM010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.470"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..711)
                     /locus_tag="N7C74_005045"
     CDS             complement(<1..711)
                     /locus_tag="N7C74_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU2434705.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIC"
     gene            complement(717..>1025)
                     /locus_tag="N7C74_005046"
     CDS             complement(717..>1025)
                     /locus_tag="N7C74_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU2434706.1"
                     /translation="VISRWRIEQCSELSAVSASFVLSTPTETDGAVFPGRIMLANTCT
                     WTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGFLSINKLSQ
                     "
BASE COUNT          227 a          326 c          270 g          202 t
ORIGIN      
        1 gcaaatcccc gtaaaggcag atgcgcgcca tgcccggtga cgccagaggg agtgtgtgcg
       61 tcgctgccat ttgtcggtat acctctctcg tttgctcagt tgttcaggaa tatggtgcag
      121 cagctcgcca tcaccacagt aaatggcggc atgattcggc accgatgaac caaaacagca
      181 cagcagcaca tcgcccggtt gtgctgatga caacggcacc tgatacagcc ctgtggcctc
      241 cagattatcc agatagagat tctgaccgtg acgccaccag tcatcctcgc gatgaaaatc
      301 cggcatctca atccccgcca gatgataagc atcccggaac agcgtgtaac agtccgtcac
      361 cccgtgctca aagcgccgcc cggtgagatg cggcacacag cggaacttat gaatcgtccc
      421 ccggcagacc agccaccacg gcaaatcact ctgcacctgc agccgccggt cggcctcact
      481 cagccagggc agaccaccgg ggtggctgtg gaccagcgcc acaatctcac cctgcatttc
      541 tgcctgcagc cagtcttccg gcgacatacg gaaatacgcc tccggctcac cggagatatt
      601 cacgcagggg aaatatcttt ccccctccgg cgtgcttacc acgaagccgc acgactccgc
      661 tggcgcacat cgccgggcgt gcgccagaat cgctgattct gtctgtgtca tgggatttac
      721 tgcgaaagtt tgttaatgga aaggaagccg ccaaagttgc cgacgttatt gcggaactta
      781 caaccgctca ggcatttgct gcatttatcc ttcgtgatat cggacgttgg ctggtcatat
      841 tcatccgcga ccgccggacc gtgataaccg cactcatcgc cgcgataggt ccaggtgcag
      901 gtgttggcca gcatgatacg tcccggaaaa acagcgccgt ccgtttccgt cggcgtggac
      961 agtacaaaag aggcactcac cgcgctcagt tcgctgcact gctcaatgcg ccagcggctg
     1021 atcac
//
LOCUS       DBFIFM010000461         1025 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.471, whole genome shotgun sequence.
ACCESSION   DBFIFM010000461 DBFIFM010000000
VERSION     DBFIFM010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.471"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..96)
                     /locus_tag="N7C74_005047"
     CDS             complement(<1..96)
                     /locus_tag="N7C74_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434707.1"
                     /translation="MVCLYNFGVRAAMDNSKHSDEAEKLLAELSAR"
     gene            complement(129..>1025)
                     /locus_tag="N7C74_005048"
     CDS             complement(129..>1025)
                     /locus_tag="N7C74_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU2434708.1"
                     /translation="RLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAEYIPERKLH
                     LYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSLRLPGQQYDEELGL
                     YYNRNRYYDPLQGRYITQDPIGLEGGWNLYQYPLNPIEHIDPLGLALDLNYYSPSDPI
                     YKGSLNVREFPTGFTVGGHGSPTSMSDDRIKKGSDLTIKQLASDIRANPKYHEGMPVV
                     LFSCETGKGKNSFAQKLANELDATVIAPDEIIWIWPDGNYAIMGQTARITIGGKDNGV
                     FELVPDEKQPGDFHKFTPTGSK"
BASE COUNT          240 a          291 c          200 g          294 t
ORIGIN      
        1 tctagcagaa agctccgcca ataatttttc cgcctcatca gaatgttttg aattatccat
       61 agcagccctc actccaaagt tataaagaca taccatgata atcaacaaaa aagtaaatac
      121 tttcattact atttgcttcc tgtaggtgta aacttatgaa agtcccctgg ttgtttctcg
      181 tctggcacca attcaaacac cccgttgtcc ttaccgccta ttgttatcct agctgtttgc
      241 cccataatag catagtttcc atccggccaa atccatatta tttcatcagg agcaatcact
      301 gtagcatcaa gttcgttagc tagtttctgt gcaaatgaat ttttgccttt tcctgtttcg
      361 caggaaaaca agactacagg cataccttca tgatatttag gattcgctct tatgtcgcta
      421 gctaattgtt ttattgtcag atcacttcct tttttgattc tgtcatcact catagatgta
      481 ggcgaaccat gacctccaac tgtaaaaccc gttggaaatt cccttacatt aagagaacct
      541 ttatatatag gatctgacgg agaataataa ttcaaatcaa gtgctaaccc caacggatct
      601 atatgttcaa taggattgag tgggtactga tacaggttcc atcccccctc cagccctatc
      661 gggtcctggg tgatatatct cccctgcaac ggatcatagt accggttgcg gttgtagtac
      721 agccccaact cctcatcata ctgctgcccc ggcagacgga gtgactgttg aaggtgctgc
      781 gcgctggttt cgcccagcag gtttccccac tcgtcatact ccccctgcca cgccgtttcc
      841 ccttccgggc tgatgagcgc cagcggcagt ccccggtggt cgcagtggta aagatgaagt
      901 ttcctctccg ggatgtattc cgcctccagc tgcgcggcca tctgctccgc cgtcagcccg
      961 cactgcgcaa gccactgctg gctttcttca ctcacgctgc cctgccgcag ctcccgctcc
     1021 agcct
//
LOCUS       DBFIFM010000462         1016 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.472, whole genome shotgun sequence.
ACCESSION   DBFIFM010000462 DBFIFM010000000
VERSION     DBFIFM010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.472"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            297..458
                     /locus_tag="N7C74_005049"
                     /pseudo
     CDS             297..458
                     /locus_tag="N7C74_005049"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            442..507
                     /locus_tag="N7C74_005050"
     CDS             442..507
                     /locus_tag="N7C74_005050"
                     /inference="COORDINATES: protein motif:HMM:NF033646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAST1 family heat-stable enterotoxin"
                     /protein_id="HGU2434709.1"
                     /translation="MWCATACASCHGRNTKPSLAT"
     gene            623..880
                     /locus_tag="N7C74_005051"
     CDS             623..880
                     /locus_tag="N7C74_005051"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU2434710.1"
                     /translation="MATFFAYPADIRKVIYTTNAIESLNSVIRHAIRKRKVFPTDESV
                     KKVVWLAIQAASQKWTMPLRDWRMAMSRFIIEFGDRLDGHF"
     gene            complement(927..>1016)
                     /locus_tag="N7C74_005052"
     CDS             complement(927..>1016)
                     /locus_tag="N7C74_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HGU2434711.1"
                     /translation="VEGCSDDFNPFDIIRVPDLTYNKGSLQCG"
BASE COUNT          302 a          232 c          231 g          251 t
ORIGIN      
        1 aaaaacaact ttttatcttt ttgtttcgct caaattagta taaaaagccg aacaacaaac
       61 aataaaaaac cattaacatc aatagattat gatggcttca atctaaataa taggctatat
      121 aatactgcaa gacacaacac atgctgtcac tatgagtcaa ctatttagat gatattagtg
      181 acctgtttca gagcattagc gcaaggcgat tctttgtctt cttgcactaa ttttttatca
      241 taaaaatgtt cctagcactg ggcatcaata tcgcaggtca gaaagagctc ctggggatgc
      301 ggctggccga aaatgaaggg gcgaatttct ggttcaatgt gctgactgaa ctgaaaaacc
      361 gcggtctgaa cgatatcctc atcgcctgtg tgtatggcct gaaagaattc ccggaggccc
      421 gcatccagtt atgcatcgtg catatggtgc gcaacagcat gcgcttcgtg tcatggaagg
      481 aatacaaagc cgtcactcgc gacctgaaag cgattagcct cccacagaag aggcaggcca
      541 gcaggcactg gaagcgtttg ctgcggcctg ggactgccgc tatccgcaga taagccggtg
      601 ctagctgtca aactggacta acttggcgac gtttttcgct tatccggcag atatccgcaa
      661 agtgatctac acaacgaacg ccatcgagtc gctgaacagt gtgatccggc atgccatcag
      721 gaaacgcaag gtgttcccga cggacgagtc agtaaaaaaa gtggtgtggc tggcaatcca
      781 ggccgcgtca cagaaatgga caatgccgtt aagggactgg cgaatggcaa tgagccgctt
      841 tattatcgag tttggtgacc gtcttgacgg tcacttttaa gaaaaggcat ttacacagaa
      901 tcctaaacag gctcacaggc ctctaatcat ccacattgta aagatccttt gttgtaagta
      961 agatctggta ccctaataat atcaaaagga ttaaaatcat cactgcatcc ttccac
//
LOCUS       DBFIFM010000463         1015 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.473, whole genome shotgun sequence.
ACCESSION   DBFIFM010000463 DBFIFM010000000
VERSION     DBFIFM010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.473"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(281..516)
                     /locus_tag="N7C74_005053"
                     /pseudo
     CDS             complement(281..516)
                     /locus_tag="N7C74_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(675..>1015)
                     /locus_tag="N7C74_005054"
     CDS             complement(675..>1015)
                     /locus_tag="N7C74_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HGU2434712.1"
                     /translation="MHSNDNRIELLQRRLHSCSFLVNIEEMSYIDEALQCPITLAIPQ
                     RGVFLRNAEGSRVCSLYDEMALSRIINDGMHHPLSREPITLSMLVAREQCEFDCSIGH
                     FTVRSDCYSV"
BASE COUNT          308 a          234 c          178 g          295 t
ORIGIN      
        1 aaaacaaaaa tttctgccct atacacgttc caaaaaattc aacagcaaag agagtctgct
       61 gacgagttgt tgattcactg attaatacgt aaaattgata actgctggaa atcatacaat
      121 actcgtactt tcgaaagttc gctaaccagg tgctacacgc gaggtgttgc agttttcttt
      181 acgatttatc attcaatcct aatccatgca tgacatgtgg tgcctgtggg gtgatcctac
      241 ctacgtaatg tggacacagc cctaatcaag gttctgtttt tcaaaacagt tccgtgctga
      301 gacagccaca agtactgtgt cggcaccacc gattgtaatc acactagatc taattaaaca
      361 cagccgtctg cattatttcc cgactcataa aatattagcc gtaaatgcat tcgactttta
      421 gtgagtggaa gaaactttcc gccctagcat tatcgtaaca gcgccccttt gcactcatcc
      481 tgtcatgcag gtatgccgct tcagaagcgc ctgataatgc gttgaaatca ggacggtggc
      541 tacttgctat tcagttcacg atacatccct taaggaccag cgaatagcaa tgagtcactt
      601 tattatcaag tttgatgacc gctttgatgc taacttttaa taagagtctc ttcacacaaa
      661 atcgtgtacc gagtctacac tgaataacaa tcactcctca ccgtaaagtg accgatactg
      721 caatcaaact cacactgctc tctggccaca agcattgata atgttattgg ctctctgctt
      781 agtgggtgat gcatcccatc attaattata cgagaaagag ccatttcatc atataaacta
      841 catactctgg aaccttcagc atttcttaaa aaaacacctc gttgaggaat tgccagcgta
      901 atcgggcact gtaatgcttc atctatgtaa gacatttctt caatattaac taaaaatgaa
      961 catgaatgta atcgccgctg taacaattca attctgttat cattactatg cataa
//
LOCUS       DBFIFM010000464         1012 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.474, whole genome shotgun sequence.
ACCESSION   DBFIFM010000464 DBFIFM010000000
VERSION     DBFIFM010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.474"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..795)
                     /gene="escQ"
                     /locus_tag="N7C74_005055"
     CDS             complement(<1..795)
                     /gene="escQ"
                     /locus_tag="N7C74_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ring protein EscQ"
                     /protein_id="HGU2434713.1"
                     /translation="MKPLSSQLNMKINDFYLPLLPVIGTGRLYITSKGHACHAYFREV
                     SGHGIRFTLTYSGYEGRFWISEEQFIQWCQELFPYSESRLIPEDTIKLMILWVMQTAL
                     PEGDVSVDDVQFTMLNKDIYPVIENNNGENRLNVIILETTVQSLQYLINDNWQLVPHS
                     NALFFDGYIVPGWTDYPVTELRVGDSLRLYHVDDSQERRCWIVINTPLATVNLSDNNL
                     SVTDVLAADLLSALSNETVMNRIYCAIGTVHIDIHMLRNVKKDDIIN"
     gene            complement(758..>1012)
                     /gene="escP"
                     /locus_tag="N7C74_005056"
     CDS             complement(758..>1012)
                     /gene="escP"
                     /locus_tag="N7C74_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle length
                     regulator EscP"
                     /protein_id="HGU2434714.1"
                     /translation="LFYSSGRRRRYFEEGEHSFSILCGRLRGIVLTIKCSNGIIYLSI
                     KVSPNNRNHVFLYHKKDYVFDKLKEIFPDEAIEFTIEYEN"
BASE COUNT          331 a          215 c          159 g          307 t
ORIGIN      
        1 attaataatg tcatcttttt taacattacg caacatgtgt atatcaatat ggactgtacc
       61 gatcgcacaa taaatgcgat tcatgacagt ctcattgcta agagcagata acagatccgc
      121 agccagaaca tccgtgacag ataaattatt atcactcaga tttactgttg ctaaaggggt
      181 attaataaca atccagcacc ttctctcttg actatcatca acatgataga gtctcagact
      241 atcaccaact ctcaactctg taactggata atcagtccac ccaggtacga tatagccatc
      301 aaaaaaaaga gcattagaat gtggaactaa ttgccaatta tcattaatca gatattgtaa
      361 actttgtacc gttgtttcga gtataattac attaagtctg ttttctccat tattattttc
      421 tattaccgga taaatatctt tatttagcat cgtaaattga acatcatcaa ctgacacatc
      481 accttccggt aaggcagtct gcattaccca taagatcatg agttttatcg tatcttccgg
      541 tatcagccgg ctttcgctat acgggaataa ctcttggcac cactgaatga actgttcttc
      601 tgatatccag aaacgcccct catagcccga gtatgtcaac gtgaaacgaa taccatgacc
      661 gctgacctca cgaaaatatg catggcaagc atgacctttg gacgtaatat agaggcgacc
      721 ggttccgata actggcaaca acggcaaata aaaatcgtta attttcatat tcaattgtga
      781 actcaatggc ttcatcagga aagatttctt ttaacttatc gaaaacatag tcttttttat
      841 gatataaaaa aacatgattt ctattatttg ggcttacttt aatagacaga taaatgattc
      901 cgttactaca tttaattgtt aaaacaattc ctcgtaatct tccacaaaga atactaaacg
      961 agtgctcccc ttcttcaaag tagcgtcgtc ttcggcccga ggagtaaaac aa
//
LOCUS       DBFIFM010000465          994 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.476, whole genome shotgun sequence.
ACCESSION   DBFIFM010000465 DBFIFM010000000
VERSION     DBFIFM010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.476"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(112..801)
                     /locus_tag="N7C74_005057"
     CDS             complement(112..801)
                     /locus_tag="N7C74_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HGU2434715.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(816..938)
                     /locus_tag="N7C74_005058"
     CDS             complement(816..938)
                     /locus_tag="N7C74_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HGU2434716.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
BASE COUNT          264 a          252 c          191 g          287 t
ORIGIN      
        1 gcagccagca tgtttaccgt acaagtacaa ctcagggcat aaaaaaaccc actcggcagc
       61 gggtttatac attttttaca acataccaaa tttgcatgaa gtatatggct tttaatccag
      121 ttttgcaata ttttgctgta aaaatgctgc cttttgtttt gaacgtgttc tcgtcacaag
      181 caataaagca tcactatcaa gctgtagaaa aatgtgcttc attgcaaccc agcgttcagt
      241 aaatgtctcg gaccagtttt ttgttgtcac tcccaccaat gatgccagtg tctggtattc
      301 ataggcctca cgccctgcaa gttcgctctt cacatcctgt gcagccagcc agattaacgt
      361 cttcaggcga tccagtgtct tacctgcaat ttttctgtta cttaacaaat ctttaaactc
      421 gctccatgcc cattgcgtta tggtgacctg atgcccccat cgaacgcttt cgctgtaaca
      481 ccaaagcaac catgctttct gatgttcatc gagagacaaa accgcgcggc gccatgaaga
      541 ggttgagaat tcaaccgggc tgaccaaagc aatggatgaa ccttttgcgt acgactgctt
      601 accggaagtc ggcgtattat ccagcgtaat catcttgcca gttaccacat ccagaatgcg
      661 cggcttcttt cgtttgtatg taccagtatc aaattgtgca tgctcctgcc aggcttcgag
      721 ctggcctttc gttgctccgt tcaagtcagc agtagctgcc ataagttgct cacgaacata
      781 ctgtaaatat tgggtattca tgcagtaaat cctttctata ttttggcata attcttcaac
      841 attcggtaat cgttcaaaac cgaatcgggg aaacgacata agcacaggag cccccagcga
      901 cagcgaagga gttctgatat ataagactca gacatcattc attccccggt tctccaatat
      961 ctgtttcact catcatccat aactacctgt aatt
//
LOCUS       DBFIFM010000466          994 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.477, whole genome shotgun sequence.
ACCESSION   DBFIFM010000466 DBFIFM010000000
VERSION     DBFIFM010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.477"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            314..865
                     /gene="ybcL"
                     /locus_tag="N7C74_005059"
     CDS             314..865
                     /gene="ybcL"
                     /locus_tag="N7C74_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HGU2434717.1"
                     /translation="MKTLIVSTVLAFITFSAQAAAFQVASNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGVPEGTKSFAVTVYDPDAPTGSGWWHWTVVNIPATVTYLPVDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTEKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            875..>994
                     /locus_tag="N7C74_005060"
     CDS             875..>994
                     /locus_tag="N7C74_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGU2434718.1"
                     /translation="MLARGKTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKK"
BASE COUNT          311 a          160 c          229 g          294 t
ORIGIN      
        1 ttaatcattt gttaggtgag aactcttggt cgcagattca aatactgaaa atacgtgaca
       61 aattattatg agcaaaatgg tgtatgtcac gtattttgaa tggtaggtta aaaaataaca
      121 ccgactttcg taggtattac taataataaa gcagagtttt tagatagtat caatgtgctt
      181 tgtgtatatt gtggcaaata attgggttgg gggtacaatt gtgattgctt ttgcatgaac
      241 attgcgcctt tatgcataat gagataaagg aatatcaaat aaaataacga taggtcataa
      301 caaagaggtt tttatgaaaa cacttatcgt ttcaactgta ttggcattca taacattttc
      361 tgcgcaggct gcagcatttc aggtcgctag taatgaaata aaaacaggag agcaacttac
      421 aacgtctcat gtcttttctg gatttgggtg tgaaggtggt aatacatcgc cctcattaac
      481 ctggtctggt gttcctgaag gtaccaaaag ctttgccgta actgtatatg atccagatgc
      541 acctacaggc agtggttggt ggcattggac tgttgttaat attccagcaa cagtaacata
      601 tttgcccgtt gatgcaggga gacgtgatgg aacaaaactg ccgactggtg ctgttcaagg
      661 ccgaaatgat tttggctatg ctgggtttgg tggcgcatgt cctcctaaag gagataaacc
      721 acatcattac cagtttaaag tatgggctct aaaaactgaa aagattcctg tagattctaa
      781 ctccagcgga gcgttagttg gttatatgct taatgctaat aaaatcgcaa ccgctgagat
      841 aacaccagtt tatgagataa agtagggtga gagtatgctg gcaagaggta agactaactt
      901 aaagatcgaa gaaatacgga tgcataaaca tcatgagatt catagggtta agcctcttat
      961 gccagctttg tgtcgtatcc gtcagggaaa gaaa
//
LOCUS       DBFIFM010000467          990 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.478, whole genome shotgun sequence.
ACCESSION   DBFIFM010000467 DBFIFM010000000
VERSION     DBFIFM010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.478"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..295)
                     /locus_tag="N7C74_005061"
     CDS             complement(<1..295)
                     /locus_tag="N7C74_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU2434719.1"
                     /translation="MKGLLSLLISSMIFPVHAGMVIFGTRIIYPAEQKEVMVQLMNQG
                     NRSSLMQAWIDDGDTSLPPEKIQVPFMLTPPVVKVAANSGQQIKIKIMPNKLPS"
     gene            complement(383..925)
                     /locus_tag="N7C74_005062"
     CDS             complement(383..925)
                     /locus_tag="N7C74_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2434720.1"
                     /translation="MKHSIIAVAVLSSVFMSAGIFAAEVDTGTLTIKGNIAESPCTFV
                     AGGDSVSINMPTVPTTVFDGKAKYSTYDGAVGVTSSMLKITCPKTIDGVKLSLITNEK
                     ILGNDKAISSSNDTVGYYLYLGDSSDVLDISAPFNIESYKTADGQYAIPFKAKYLKLT
                     DSGVASGTVNSSLVMRVAQD"
BASE COUNT          297 a          206 c          181 g          306 t
ORIGIN      
        1 tactgggtaa tttattaggc atgattttaa tttttatttg ttgcccagaa ttggccgcta
       61 ctttcactac tggtggcgtt aacatgaaag gaacctgaat cttttctggc ggcagagacg
      121 tgtcgccatc atcaatccac gcctgcatca gcgaagagcg gtttccctga ttcatcagtt
      181 gtaccatgac ctctttctgc tctgccgggt aaatgatacg ggtcccgaaa ataaccattc
      241 cggcatgcac ggggaatatc atagaagaga tgagtaaaga taataaccct ttcatcttaa
      301 tgcctcttat atttatgctg gccgccatga atatggcggc cattttaact tactgaaaaa
      361 acaagatgat tattttaaat atttaatcct gggcaacacg cataaccaat gaagagttaa
      421 ctgtaccaga tgcaacacca ctgtcagtca acttcagata ttttgcctta aacggtattg
      481 catattgtcc atcagcagtt ttataacttt caatattaaa tggtgcggaa atatccagta
      541 cgtcactgct atctcccaaa tagagatagt atccaactgt gtcattacta ctggatattg
      601 ccttatcatt accaagaatt ttctcgttgg tgatcaacga aagttttacg ccatcaatag
      661 tcttcggaca agtaattttc agcatactgc tggttacacc aaccgcgcca tcataagttg
      721 aatatttcgc tttaccatca aatacagtgg tgggtacagt cggcatatta atactgactg
      781 aatcaccgcc agcgacaaat gtacatggtg attctgcaat attaccttta atggttaaag
      841 ttccggtatc tacttcagca gcgaaaatac cagcactcat aaatacagaa gataagacag
      901 cgacagcaat aattgaatgt ttcatgatgc attccttaat aagtttattt tgttttgtta
      961 agagtctgta tctcttggaa acaaatttct
//
LOCUS       DBFIFM010000468          984 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.479, whole genome shotgun sequence.
ACCESSION   DBFIFM010000468 DBFIFM010000000
VERSION     DBFIFM010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.479"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..>984)
                     /locus_tag="N7C74_005063"
     CDS             complement(<1..>984)
                     /locus_tag="N7C74_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU2434721.1"
                     /translation="NGQAEFINNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGI
                     TTTPGVYDSTGLLNLKELPGHLGILGGGYIGVEFASMFANFGSKVTILEAASLFLPRE
                     ERDIADNIATILRDQGVDIILNAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPA
                     TASLHPENAGIAVNERGAIVVDKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDE
                     LLGEGKRSTDDRKNVPYSVFMTPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVM
                     NDTRGVLKAIVDNKTQRILGASLLCVDSHEMINIVKMVMDAGLPYSILRDQ"
BASE COUNT          254 a          258 c          208 g          264 t
ORIGIN      
        1 atctgatcgc gtaatatgct ataaggcagc ccggcatcca tcaccatttt cactatatta
       61 atcatctcgt gggagtcaac acacagcagt gatgccccta atatacgttg ggttttatta
      121 tcaacaatcg cttttaatac cccacgggta tcgttcatca ctctggcgcg cggaattgca
      181 gctacaggca atgtcaccac ctgaatatca gcaccactct ctctggcttg ttcttctgtc
      241 ataccaaccc tggacagggg cggtgtcata aatacggaat aaggcacatt tttccgatca
      301 tcagtactac gtttgccttc acccagtaac tcatcacgta caatgcggta atcatccagt
      361 gatatgtaag taaattgcag cccgccggta acatctccca tcgcccaaat attgtctgcg
      421 gtggtatgta atcgcttgtc aacgacaatt gccccgcgct cgtttactgc gataccggca
      481 ttttctggat gtaacgaagc ggtagccggt tgacgaccgg aagctattaa cagtgcatcc
      541 accgccagtt gggcgtgctc gctatgcact tgcacttgat tttcatggtg actgattcgc
      601 tccacatggg cattgaggat aatatcgacg ccctgatcgc gtaaaatcgt cgcgatatta
      661 tcagcaatat cccgttcttc ccgaggcaaa aacagcgaag ctgcttctaa aatggttacc
      721 ttgctgccaa aattagcgaa catagaggcg aactcaacgc caatatatcc gccgcccaaa
      781 atacctaaat gcccaggcaa ttcttttaga ttaagtaatc cggtgctgtc atatactcct
      841 ggcgtggtgg taattccagg aattggcgga accacggttt gtgcaccggt attaataaaa
      901 attttctcgc catgaatttc cagatttccc tcaggccgat gaacacgcag gctatgatta
      961 ttgataaact ccgcctggcc gttg
//
LOCUS       DBFIFM010000469          981 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.480, whole genome shotgun sequence.
ACCESSION   DBFIFM010000469 DBFIFM010000000
VERSION     DBFIFM010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.480"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            126..>981
                     /locus_tag="N7C74_005064"
     CDS             126..>981
                     /locus_tag="N7C74_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HGU2434722.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSADTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNREGLLAANGAVR
                     LDALSLDNR"
BASE COUNT          234 a          229 c          270 g          248 t
ORIGIN      
        1 ggtgattgtt ggtatgacat gaataaaagg tttttcgttt gactggaaaa ttatgttgca
       61 ggaacgagag gactgaggaa caaagcttat tccggcgctg tccgtccttg ctgtcactga
      121 cactgatgtc tgctttatta tcgcctttat ctcttcaggc agcggatgtc cggcgtagcg
      181 gagatgaagc atttatcatt cagcagcagc gtcaggaagc ccttgagcaa caactgacgc
      241 cttcagcccc tgatgttcgc ctttctgcac ctggctcttt tgcccataag attaattttc
      301 ctgttgaaac gccctgtttt cagattaaac agacggaact gaagggggct gatgcgttac
      361 cacactggct gcctttacaa aaaatcgcca acggggcggt cgggcattgc ctgggggcga
      421 aaggaattaa tctgctgatg agtacattgc agaaccgtct ggtcgatcat ggttatgtca
      481 ccacccgtgt tctggcacct tcgcaggatt taaaaagcgg tatcctccgg ctggttatta
      541 ttcccggtgt tgtgcgacat gtgcgtctga caccggacag tgatgactat attcagttgt
      601 attcctcatt cccggcacac gaaggttctc tgctggattt acgggacatt gagcaggggc
      661 tggatttagg taacagccgg atacagggac aacatactga gctgaatgca accagtggaa
      721 atctgtctac acagaatgcg caactgagtg ccgatacgct ttccgcccgg actgccgggc
      781 agttcagcag taatggcggt acgataaatg ccgacacact gcagatatcg gcacaaagcc
      841 tgtcaaatcg taaaggcagt ctgattcaga cgggaacagg ggatttttcg ctgagtctgc
      901 cgggaagcgt ggataaccgg gaagggctgc ttgcggcaaa tggcgcggtg cgtctggatg
      961 cactgagcct tgataatcgc a
//
LOCUS       DBFIFM010000470          978 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.481, whole genome shotgun sequence.
ACCESSION   DBFIFM010000470 DBFIFM010000000
VERSION     DBFIFM010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.481"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(1..136)
                     /locus_tag="N7C74_005065"
                     /pseudo
     CDS             complement(1..136)
                     /locus_tag="N7C74_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            286..954
                     /locus_tag="N7C74_005066"
     CDS             286..954
                     /locus_tag="N7C74_005066"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU2434723.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
BASE COUNT          289 a          220 c          190 g          279 t
ORIGIN      
        1 ctactgatgt atcaacacga ttcaacaata cccgatattt attccatgcc tccagcaacg
       61 atctttcttc ctccgttgcg atttccagat ctacagcatc ctgcagtggc gcaatatact
      121 cactgaattc ctggatgtag aactgtgtgg tgacggtctt ccagccattc ggctcctgct
      181 gtatcgcagc ataccaggct atttcaatat cgctatgctg cggcagcatt taaccccttg
      241 taattcatcg ccataattga tttaattcac aaataaaact ataacatggt gaaattaatg
      301 aaaaaaaaca cagatgatgg ggctaaaatt tacacaccac ttaccctaaa gctttatgac
      361 tggtgggttt tgggagtatc aaatcgtctt gcatggggat gtcctacaaa ggaacacctt
      421 cttccacact ttctggaaca tttaggtaac aaccatctgg atattggtgt tggaactggg
      481 ttttacctta ctcacgtacc tgagagtagt ctgatatctt taatggattt gaacgaagct
      541 agcctgaacg cggcatctac aagggctggg gaatcaaaaa ttaaacataa aattagccat
      601 gatgtttttg aaccttatcc cgcggcgtta catggtcaat ttgattccat ttccatgttt
      661 taccttcttc actgcctgcc tggaaatata tctacaaaaa gctgtgtaat acgcaatgcg
      721 gcgcaggcct taactgacga tggaactcta tacggagcca caattcttgg cgatggagtt
      781 gtgcacaata gcttcggtca aaaactgatg cgcatttaca atcagaaagg catcttttca
      841 aacacaaaag attccgaaga aggcttaaca catatactct cagagcattt cgagaatgtt
      901 aaaaccaagg ttcaaggtac tgtagtaatg ttttccgctt cagggaaaaa atagcatcca
      961 accgcagcac gttcttgc
//
LOCUS       DBFIFM010000471          970 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.482, whole genome shotgun sequence.
ACCESSION   DBFIFM010000471 DBFIFM010000000
VERSION     DBFIFM010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.482"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            107..931
                     /gene="gadX"
                     /locus_tag="N7C74_005067"
     CDS             107..931
                     /gene="gadX"
                     /locus_tag="N7C74_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HGU2434724.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDDHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDCSAEGIRL"
BASE COUNT          283 a          177 c          209 g          301 t
ORIGIN      
        1 cctgttctcc cgcttcgttt aaatttattt atcaatcaat ttgacttaag agggcggcgt
       61 gctacattaa taaacagtaa tatgtttatg taatattaag tcaactatgc aatcattaca
      121 tgggaattgt ctaattgcgt acgcaagaca taaatatatt ctcaccatgg ttaatggtga
      181 atatcgctat tttaatggcg gtgacctggt ttttgcggat gcaagccaaa ttcgagtaga
      241 taagtgtgtt gaaaattttg tattagtatc aagggatacg ctttcattat ttctcccgat
      301 gctcaaggag gaggcattaa atcttcatgc acataaaaaa gtttcttcat tactcgttca
      361 tcactgtagc agagatattc ctgtttttca ggaagttgcg caactatcgc agaataagaa
      421 tcttcgctat gcagaaatgc tacgtaaaag agcattaatc tttgcgttgt tatctgtttt
      481 tcttgaggat gatcacttta taccgctgct tctgaacgtt ttacaaccga acatgcgaac
      541 acgagtttgt acggttatca ataataatat cgctcatgag tggacactag cccgaatcgc
      601 cagcgagctg ttgatgagtc caagtctgtt aaagaaaaaa ttacgcgaag aagagacatc
      661 atattcacag ttgcttactg agtgtagaat gcaacgtgct ttgcaactta ttgttataca
      721 tggtttttca attaagcgag tcgcagtgtc ctgtggatat cacagcgtgt cgtatttcat
      781 ttacgtcttt cgaaattatt atgggatgac gcccacagag tatcaggagc gatcggcgca
      841 gggattgccg aaccgtgact cggcggcaag tattgttgcg caagggaatt tttacggcac
      901 tgactgttct gcggaaggaa taagattata gagttttact cagacataaa aaaaacccgg
      961 cataggggac
//
LOCUS       DBFIFM010000472          952 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.483, whole genome shotgun sequence.
ACCESSION   DBFIFM010000472 DBFIFM010000000
VERSION     DBFIFM010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.483"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            141..776
                     /locus_tag="N7C74_005068"
     CDS             141..776
                     /locus_tag="N7C74_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HGU2434725.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AAERISEQGLYAMRDVQVARLALFHGDPEKAKKLTNEASALLSDDSTEWAKFAKPGKK
                     TNVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVM
                     ENQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          303 a          196 c          213 g          240 t
ORIGIN      
        1 aagactaaaa tacttcttgc gataataact acaactgtaa gataaccctt tcaaaatgac
       61 cgttgctctc tgatttctca tttcatgctc acccaatatg atggcggcgt tttctaaaac
      121 tgttaaagaa tgaggtaagt atgaaacgtt taattatggc tacgatggtc acagctattc
      181 tggcatcttc aactgtatgg gctgctgata atgctccggt agcggcacaa cagcaaactc
      241 agcaagttca gcaaacccag aaaacggcag ctgcagcaga gcgtatctct gaacaaggtt
      301 tatatgcgat gcgtgacgtg caggtagcgc gcctggctct gtttcatggc gatccggaaa
      361 aggcaaaaaa actgaccaat gaagcttcag ctttgttgtc agatgacagt accgaatggg
      421 caaagttcgc taagccaggt aaaaagacca atgtcaatga tgaccaatat attgtcatta
      481 atgcatctgt cggaatttct gagagttatg tagcgacacc cgaaaaagaa gccgcgataa
      541 aaattgccaa tgaaaaaatg gcgaaagggg ataaaaaggg cgcaatggaa gagcttcgcc
      601 tggccggtgt tggtgtaatg gaaaaccaat atctgatgcc gcttaaacag actcgtaatg
      661 cacttgctga tgcgcagaaa ctgcttgata aaaagcaata ctacgaggca aacctggcgc
      721 ttaaaggtgc ggaagatgga atcatcgtcg atagcgaagc actgtttgtg aactaatgct
      781 gacaggcagt atattgaggt aaaggattta actccctcca gccgacaatt ttttatgacc
      841 acaatacgac aggttgtggt cataaaatta tttggcgctc gctgttctat aaactcgcat
      901 tttatattcc tcaaagtaaa ccgcaataat cccgtgtcat cacaaatcat tt
//
LOCUS       DBFIFM010000473          946 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.484, whole genome shotgun sequence.
ACCESSION   DBFIFM010000473 DBFIFM010000000
VERSION     DBFIFM010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.484"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(474..692)
                     /gene="ydfC"
                     /locus_tag="N7C74_005069"
     CDS             complement(474..692)
                     /gene="ydfC"
                     /locus_tag="N7C74_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HGU2434726.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKINAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(764..>946)
                     /locus_tag="N7C74_005070"
     CDS             complement(764..>946)
                     /locus_tag="N7C74_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434727.1"
                     /translation="VNDFVIELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDFATT
                     FWARKLDCIHNQFPLE"
BASE COUNT          217 a          246 c          227 g          256 t
ORIGIN      
        1 cccataacac tgtttttggt tttaactgtt ccgcgtgcgc tcagccgcat tcaccgcatc
       61 acaaaattca ctttaaaaag ggcggcagag cagtcacgga gtaaaactga taccgccaaa
      121 cgtcaccaga aaattgataa cagagggcgt tgcagcgggg ttgtcactta agcgtatggt
      181 caacctgaca acccggtgtc ctcaacgggg gaaggaataa cccctccata cttaccgccg
      241 cgccatttcg cggattgcca caaccggaag cgcacggtcg acgaaaattt aacgacaggc
      301 tatctatgaa ccagctacct cgccgtgcgc tttcgcgtta tggtctgact tttcagggaa
      361 atatcctttc agtaaactgt cagtgccgta tgctcacccg tgtccggcgc acgcactcca
      421 cctcacccgt ggagaactcc ttaattacca accttagctt cgttggttag ctattaacgc
      481 gggtatgtaa tcattctggc aatgcttaat gccgctgctt tttccagatt ggtgatatcc
      541 tgctccagag cggacagatt ttcagcctgc ttagccctgg cttcattggc ccatttcaga
      601 tcctgcgctg cattaatttt ctggtgcatc cactcataaa gttcatcatc ggtatagtct
      661 ggcgcgatga tgacgggttc tcgtttctgc atgtcggctc cttgtggtta gcgttgcctg
      721 cttttaacca cgtcaggcga ggtggtatcc tctgaggggt ctgttactcg agaggaaatt
      781 ggttatgaat acaatcaagt tttcttgccc agaatgtggt ggcgaagtct ttgacacatc
      841 ctttaagccg cagggctctg acagtttcgc gggagccatc tgcaaaaatt gtggtcacct
      901 tgtaactgaa gatgagtcct cgcagttcga tgacgaaatc gttgac
//
LOCUS       DBFIFM010000474          942 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.485, whole genome shotgun sequence.
ACCESSION   DBFIFM010000474 DBFIFM010000000
VERSION     DBFIFM010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.485"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            2..532
                     /locus_tag="N7C74_005071"
     CDS             2..532
                     /locus_tag="N7C74_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU2434728.1"
                     /translation="MLAGTNEFIGEGDAYIPPHTGLPANSTDIAPPDIPAGFVAVFNS
                     NEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSPDRECQKWNGTSWAKDA
                     EAEKLFRVREAEETKNSLMQVASEHIAPLQDAVDLDIATEEEASLLAAWKTYRVLLNR
                     VDTTVAADVEWPVAPQ"
     gene            complement(576..>942)
                     /locus_tag="N7C74_005072"
     CDS             complement(576..>942)
                     /locus_tag="N7C74_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434729.1"
                     /translation="AAKKLNSKIINKFGCEIGLGANIGKGLTIPHHAGIVVHFAVDAG
                     ENLVLRQNTTIGQIDGDMPSSRVKIGSNVDIGANCCIIGLSRKIGDNVKIGAMSFINK
                     DIPSNCTYITKKSGVVLYK"
BASE COUNT          270 a          199 c          204 g          269 t
ORIGIN      
        1 tctgctggcc ggaactaatg aatttattgg tgaaggtgac gcatacattc cgcctcatac
       61 aggtctgcca gcaaacagta ccgatattgc accgccagat attccggctg gcttcgtggc
      121 tgttttcaac agtaatgagg catcgtggca tctcgttgaa gatcatcggg gtaaaacggt
      181 ttatgacgtg gcttccggcg acgcgttatt tatttctgaa ctcggtccgt taccggaaaa
      241 tgttacctgg ttgtcgccgg atagagagtg tcagaagtgg aacggcacat cctgggcgaa
      301 agatgcagaa gcagaaaaac tgtttcgggt acgggaggca gaagaaacaa aaaacagcct
      361 gatgcaggta gccagtgagc atattgcgcc acttcaggat gccgtagatt tggatattgc
      421 gacggaggaa gaggcatcgt tactggctgc atggaagaca tatcgggtat tgttgaatcg
      481 tgttgataca acagtagcag cggatgttga gtggccagtc gccccacaat aaaaagaaaa
      541 agccatcgac agaaatatcg atggctttat gtactctatt tatacaatac aacaccactc
      601 tttttagtta tatatgtgca gtttgatggt atatctttat ttataaaaga cattgcacct
      661 atttttacat tatccccaat tttacgtgat aatccaatga tgcaacaatt agctccgata
      721 tcaacgttac taccaatttt tactcttgaa ctaggcatat caccatctat ctgtccaatg
      781 gtagtattct gtcgtaatac cagattttca cccgcatcaa cagcaaaatg aacaacaatt
      841 ccagcatgat ggggaattgt caaccctttt ccaatatttg cgcccaatcc tatttcacaa
      901 ccaaacttgt taattatttt actgtttaac tttttggctg ct
//
LOCUS       DBFIFM010000475          929 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.486, whole genome shotgun sequence.
ACCESSION   DBFIFM010000475 DBFIFM010000000
VERSION     DBFIFM010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.486"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            85..510
                     /locus_tag="N7C74_005073"
     CDS             85..510
                     /locus_tag="N7C74_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU2434730.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLQVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TSVSAGSCKVEFRHGNMTLENPSPELLTLLIRELTGRGR"
     gene            507..581
                     /locus_tag="N7C74_005074"
                     /pseudo
     CDS             507..581
                     /locus_tag="N7C74_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(810..>929)
                     /locus_tag="N7C74_005075"
     CDS             complement(810..>929)
                     /locus_tag="N7C74_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434731.1"
                     /translation="DDDYYRERPCPGEVFGYDNTEDNDEAASDYALKINENSD"
BASE COUNT          264 a          217 c          201 g          247 t
ORIGIN      
        1 taagcgtaaa ctgaccgccg tatgtagcca tcagacgaaa attggtaact tagacgccca
       61 tctgatatag acggacatct aagtatggaa ttacaggact ggcgaaaaga acctcgtaaa
      121 aactattcga atgaattcaa acttcgtatg gtggaactgg catcacaacc aggagcctct
      181 gttgcccgta ttgcccggga acacgatatc aatgataacc tcctgttcaa atggctcagg
      241 ctctggcaga acgaagggcg tatatcacgg cgtcttcagg taacgacctc ttctgacact
      301 ggcgttgaat tattacctgt agaaataacg ccggatgagc agaaagaacc ggtggcggcc
      361 attgcgccgt ctttatccac ttccactcag accagtgtca gtgccggctc ctgcaaggtg
      421 gagttccgtc acggtaacat gacgctggaa aatccatcgc cagagctgct cacgctgttg
      481 atccgtgaac tgaccgggag gggacgatga tctcactccc gtcaggtacc cgcatctggc
      541 tcgttgccga cgttaccgat atgcgtaaat ccttcaacgg atacgacata tgctgaagga
      601 ggagcagtaa tggattaagt gataacaaat acccagaaat agaggttcaa atccagctta
      661 gttagtacat aaatactatt ttgatgaaaa ttattatatg tttcctcaga ttttaatcag
      721 gcgcccagat taaaccaagt attgagcgcc tgattatgtt tattcattaa tatctgtcat
      781 tgattgtctt attatcagct atccccgtat taatcagagt tttcatttat ttttaatgca
      841 taatcagatg ccgcctcatc attatcctca gtattatcat agccgaaaac ttctcccgga
      901 cacgggcgtt cacgataata gtcatcgtc
//
LOCUS       DBFIFM010000476          928 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.487, whole genome shotgun sequence.
ACCESSION   DBFIFM010000476 DBFIFM010000000
VERSION     DBFIFM010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.487"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..289
                     /locus_tag="N7C74_005076"
     CDS             <1..289
                     /locus_tag="N7C74_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU2434732.1"
                     /translation="GGFSATTMLFSLGASMILGGVAQMLAPKAKVPEYKSTDNGRQNT
                     YFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGDGKVVVIGRQR"
     gene            528..>928
                     /locus_tag="N7C74_005077"
     CDS             528..>928
                     /locus_tag="N7C74_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU2434733.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVQTTSQGDRNPAS"
BASE COUNT          240 a          211 c          292 g          185 t
ORIGIN      
        1 cggtggtttt tctgccacca cgatgctgtt ttcactgggg gccagcatga tactgggtgg
       61 tgtggctcag atgctggccc cgaaggctaa agtaccggag tacaaaagca cggataacgg
      121 cagacagaac acgtactttt cctcgctgga taacatgatt gcacagggga acccgatgcc
      181 ggtgccttac ggtgaaatgc tggttggctc acggcgaatc tcccaggaca tcagtacccg
      241 tgatgaaggc ggtgacggga aggtggtggt tatcgggcgg cagaggtaaa aagaataaaa
      301 aaatcccgca gtgaccgaag gctgcgggaa cagaaaatga agattaacca cagggagttt
      361 tgtttttatt ggcccgaaaa aactgtaacg cccgggaatg atatctgcca cgggggcgta
      421 cagaaaatgt gaagatattc agaattttta ttcagtcatg atacaggcat cctccgggat
      481 gcctgttgtt tttgtgcgta acagttatca cagtaaaggg tgagacaatg ggcaaaggtg
      541 gtggcaaggc gcacacgccg gttgaggcaa aggacaatct taagtccacg cagatgatga
      601 gcgtgattga tgcgattggt gaagggccga ttgaaggtcc ggtgaagggg ctgcagagta
      661 tcctggtgaa caaaaccccg ctgacggaca cggacggtaa tcctgtgata catggtgtga
      721 cagcggtctg gcgcgccggg gagcaggagc agacaccacc tgaaggcttt gagtcctccg
      781 gagctgaaac cgcactgggc gtggaagtga cgaaggcaaa gccggtgacg cgcacaatta
      841 cgtccgcgaa cattgaccgc ctgcgggtca ccttcggggt gcagtcactg gtgcagacca
      901 cctcacaggg tgaccgtaac ccggcatc
//
LOCUS       DBFIFM010000477          920 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.488, whole genome shotgun sequence.
ACCESSION   DBFIFM010000477 DBFIFM010000000
VERSION     DBFIFM010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.488"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            261..>920
                     /gene="tccP"
                     /locus_tag="N7C74_005078"
                     /gene_synonym="espF(U)"
     CDS             261..>920
                     /gene="tccP"
                     /locus_tag="N7C74_005078"
                     /gene_synonym="espF(U)"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP"
                     /protein_id="HGU2434734.1"
                     /translation="MINNVSSLFPTVNRNITAVYKKSSFSVSPQKITLNPVKISSPFS
                     PSSSSISATTLFRAPNAHSASFHRQSTAESSLHQQLPNVRQRLIQHLAEHGIKPARSM
                     AEHIPPAPNWPAPPPPVQNEQSRPLPDVAQRLVQHLAEHGIQPARNMAEHIPPAPNWP
                     APPLPVQNEQSRPLPDVAQRLVQHLAEHGIQPARSMAEHIPPAPNWPAPPPPVQNEQS
                     RP"
BASE COUNT          273 a          242 c          171 g          234 t
ORIGIN      
        1 tagatttttg gcattttggg aagatcagcg ggacataaga gacatacaac acaggaggaa
       61 tggctgtttt ttaatagtgc tttgaattcc gcatgaaacc tttcttagca gtcgaaaagc
      121 aaaacatacg ttttcttttt aacaataatt gatgagtatt gcatcgagtg tcatttatac
      181 atcgccgcat ttatttgcca ctataaaaag gctgattaca tatctatcgg agaactaaaa
      241 ttacttataa ggtaatctgc atgattaaca atgtttcttc actttttcca accgtcaacc
      301 gcaatattac agctgtatat aaaaaaagca gcttctctgt atcaccacag aaaatcacat
      361 taaatcctgt aaaaatcagc tcaccttttt caccaagcag tagctccatc agcgcaacaa
      421 ctctctttcg agccccaaac gcccattcgg catcatttca tcgacagtct actgctgaaa
      481 gttcgttaca tcaacaactt cctaatgtga ggcagcgcct gatacaacat cttgcagagc
      541 atggcattaa acctgcccgg agtatggctg aacatattcc tccggcacct aactggcctg
      601 cgccaccacc gccagtacaa aatgaacaat caagacctct gcctgatgtg gctcagcgtc
      661 tggtgcagca tcttgcagag catggcattc aaccagcccg gaatatggct gaacatattc
      721 ctccggcacc taactggcct gcgccaccac tgccagtaca aaatgaacaa tcaagacctc
      781 tgcctgatgt ggctcagcgt ctggtgcagc atcttgcaga gcatggcatt caaccagccc
      841 ggagtatggc tgaacatatt cctccggcac ctaactggcc tgcgccacca ccgccagtac
      901 aaaatgaaca atcaagacct
//
LOCUS       DBFIFM010000478          918 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.489, whole genome shotgun sequence.
ACCESSION   DBFIFM010000478 DBFIFM010000000
VERSION     DBFIFM010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.489"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..745)
                     /gene="ygcW"
                     /locus_tag="N7C74_005079"
     CDS             complement(<1..745)
                     /gene="ygcW"
                     /locus_tag="N7C74_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU2434735.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIENQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVN"
BASE COUNT          259 a          213 c          192 g          254 t
ORIGIN      
        1 cgttgacata attcgatgcc tgacttgcga gaaacacggc tgcgcccatt aaatcctgag
       61 tatcgcccca acggtttgcc ggaatatgat caagaacgcg ctgattggtt tctggattac
      121 tgcgtgtcgc cagcgtaatg tctgttgcat aatagccagg ggcgatacca tttacctgaa
      181 tattatattg acctagttca tcacaataag ctttggtgaa tccggcaaga gcatgtttag
      241 tagcagaata tgcaggtgac cattgtccac ctaagtaaga gaacaatgaa cagatattaa
      301 tgattttacc gcttttctgc gggatcataa tttttgccgc ttcgtagctt aactcgaatg
      361 cggcggtcag gttcacatca atcatcgggt cccagtcggc acgaccgaag tccagcacct
      421 tattcagctt acaaataccg gcattgttaa ccagaatatc aactgtaccg aaacgctcac
      481 agcaggcagc gataatcttc tgcggcgcgc cttctgcggt gatatccacc tgcatgaagt
      541 ccacctcaac accctgattt tcaatcattt cctttgtttc gccgttatct ttgacaaaac
      601 ttggaataaa gacatttgcg cctgctttgg ccaacgccat ggcaaatgcc tggcctaaac
      661 cgctattccc gccggtaaca attgcggttt tacctttcag ggagaaaaaa tccattgaga
      721 acgcattgag agattcgatt gacatagtta gctccaattt ccgtaaggca aaaaaaaaga
      781 aaggtaactt cccccgcgag agggaagtta cctttctcat tatctctggt aactggagat
      841 aatttttagc ataaggacat aactgctaat gtgatcgtaa tcacagtgtg atattcgtat
      901 tatcaaatag ttttaaaa
//
LOCUS       DBFIFM010000479          896 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.490, whole genome shotgun sequence.
ACCESSION   DBFIFM010000479 DBFIFM010000000
VERSION     DBFIFM010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.490"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..354
                     /locus_tag="N7C74_005080"
     CDS             <1..354
                     /locus_tag="N7C74_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HGU2434736.1"
                     /translation="KYIIDYDWKASIEIEIDHDVMTEEKLHQINNFWSDSEYRLNKHG
                     SLLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIRITDID
                     TSGIFDSDDMTIKAA"
     gene            427..591
                     /locus_tag="N7C74_005081"
     CDS             427..591
                     /locus_tag="N7C74_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HGU2434737.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNTYGYPD"
     gene            560..703
                     /gene="kil"
                     /locus_tag="N7C74_005082"
     CDS             560..703
                     /gene="kil"
                     /locus_tag="N7C74_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HGU2434738.1"
                     /translation="MDQTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILILKLRSSK
                     SIH"
     gene            658..>896
                     /locus_tag="N7C74_005083"
     CDS             658..>896
                     /locus_tag="N7C74_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HGU2434739.1"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAY
                     YIQDRLEAQSWTRHYQQIAREEKEAELADDMGKGLP"
BASE COUNT          277 a          193 c          196 g          230 t
ORIGIN      
        1 aaatacatca ttgattacga ctggaaagca tcaatagaaa ttgaaatcga ccatgacgta
       61 atgacagagg aaaaacttca ccagattaat aatttctggt cagactctga ataccgactc
      121 aataaacacg gctctttatt aaatgctgta ttaatcatgc tggcgcaaca tgctctgctt
      181 atagcaattt caagcgactt aaatgcatat ggtgttgtgt gtgagttcga ctggaatgat
      241 ggaaatggtc aggaaggatg gcctccaatg gatggtagcg aaggaataag aattaccgat
      301 atcgatacat caggaatatt tgattcagat gatatgacta tcaaagccgc ctgagcgcgg
      361 cgttaccgca taccaataac gcttcactcg aggcgttttt cgttatgtat aaataaggag
      421 cacaccatgc aatatgccat tgcagggtgg cctgttgctg gctgcccttc cgaatcttta
      481 cttgaacgaa tcacccgtaa attacgtgac ggatggaaac gccttatcga catacttaat
      541 cagccaggag tcccaaaaaa tggatcaaac acttatggct atccagacta aattcactat
      601 cgccactttt attggcgatg aaaagatgtt tcgtgaggcc gtcgacgctt ataaaaaatg
      661 gatattaata ctgaaactga gatcaagcaa aagcattcac taaccccctt tcctgttttc
      721 ctaatcagcc cggcatttcg cgggcgatat tttcacagct atttcaggag ttcagccatg
      781 aacgcttatt acattcagga tcgtcttgag gctcagagct ggacgcgtca ctaccagcag
      841 atcgcccgtg aagagaaaga ggcagaactg gcagacgaca tgggaaaagg tctgcc
//
LOCUS       DBFIFM010000480          895 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.491, whole genome shotgun sequence.
ACCESSION   DBFIFM010000480 DBFIFM010000000
VERSION     DBFIFM010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.491"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            8..574
                     /gene="slp"
                     /locus_tag="N7C74_005084"
     CDS             8..574
                     /gene="slp"
                     /locus_tag="N7C74_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HGU2434740.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            730..>895
                     /locus_tag="N7C74_005085"
     CDS             730..>895
                     /locus_tag="N7C74_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU2434741.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHS"
BASE COUNT          267 a          186 c          189 g          253 t
ORIGIN      
        1 tagtaacatg aacatgacaa aaggtgcact catactcagc ctttcatttt tgcttgcagc
       61 atgtagttca attccgcaaa atatcaaagg aaataaccaa cctgatattc aaaaaagttt
      121 tgttgctgtt cataaccagc cggggttata tgttggtcaa caagcgcgct ttggtgggaa
      181 ggttatcaac gttatcaatg gcaaaacgga tacgttgtta gaaatcgctg tattaccgtt
      241 ggatagctat gcgaagcctg atattgaagc caactatcag ggccgactgc tcgccagaca
      301 aagcggcttc cttgatccag tgaactatcg taatcacttt gttaccatcc tcggcaccat
      361 tcagggtgaa caacctggct ttatcaataa agtcccgtat aacttcctgg aagtgaatat
      421 gcagggcatc caggtgtggc atttgagaga agtggttaat accacctata acctgtggga
      481 ttacggctat ggtgcattct ggccggaacc gggctggggt gcgccttact acaccaatgc
      541 ggtgagtcag gtaacacctg agctggtcaa ataacaccac cgaaagatgc agatgctttc
      601 tcagcatctg catcatgcat tacatcaaat taatacacag taagctaact attattatta
      661 taagccctgt cctgttaatt acctttggca aactgattat aaagttaatg tccgcaccag
      721 gagtcggtta tgtttcttat aattaccagg gatacgatgt tcttcaccgc gatgaaaaac
      781 attctgagta aaggtaatgt cgttcatata cagaacgaag aagagatcga cgtaatgttg
      841 catcagaatg ccttcgtcat tattgataca ttaatgaata atgtatttca ttcta
//
LOCUS       DBFIFM010000481          880 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.492, whole genome shotgun sequence.
ACCESSION   DBFIFM010000481 DBFIFM010000000
VERSION     DBFIFM010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.492"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..790
                     /locus_tag="N7C74_005086"
     CDS             <1..790
                     /locus_tag="N7C74_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434742.1"
                     /translation="KLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTPQQLT
                     DKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMRKYYT
                     VSTFSGIPDRQKPLTCNKNKDKNENEDVARAENMNWMQALRFVAAKGHQKAIIVYQDM
                     LQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYESRVI
                     FPPAGITKTSNKELSNDHVTEAQLFNLKQKTYVSMVKDLVSRFQ"
BASE COUNT          279 a          169 c          199 g          233 t
ORIGIN      
        1 aaagctttgt tgcatatgtg tgttattctc gctgcttgca ggatgtgcct ctgaatcttc
       61 tattgatgaa aagaagaaaa aagctcaagt cacacaaagt aatattaata aaaacactcc
      121 ccagcaactg acagacaaag atttattcgg taatgaaacc actctggccg tatccgaaga
      181 ggatattcaa gcagcgcttg atggagatga gttccgcgtt ccccttaatt ctcctgtaat
      241 acttgttcaa tccggcaacc gcgcaccgga aaccattatg caggaggaga tgcgaaaata
      301 ttatactgtt tccacattct ctggtatccc ggacaggcaa aagcctctga cttgtaacaa
      361 aaacaaagat aaaaatgaga acgaggatgt tgcccgtgct gagaatatga actggatgca
      421 ggcactgcgt tttgtggctg caaaaggaca tcagaaagcg attattgttt accaggatat
      481 gttgcagaca ggaaaatatg actctgcgct gaaatcaaca gtatggtccg actataaaaa
      541 tgaaaaactc actgacgcta tctccctgcg ctacctggta cgtttcacgc tggtggatgt
      601 ggcaacaggt gagtgggcta cctggtcgcc ggtgaattat gaatcgagag taatttttcc
      661 gccggctgga ataacaaaaa cgagcaataa agaactatca aatgaccatg ttacggaagc
      721 gcaactgttt aatttgaaac aaaaaacata tgtatcgatg gtgaaagact tggtgagtcg
      781 attccagtaa tcgttattgt tatgctgccg cctgtagttt agggtggcag caaacaggag
      841 ttgagtattt ctataatgta aatatgattg tttttataaa
//
LOCUS       DBFIFM010000482          880 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.493, whole genome shotgun sequence.
ACCESSION   DBFIFM010000482 DBFIFM010000000
VERSION     DBFIFM010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.493"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(1..170)
                     /locus_tag="N7C74_005087"
                     /pseudo
     CDS             complement(1..170)
                     /locus_tag="N7C74_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
     gene            complement(457..>880)
                     /locus_tag="N7C74_005088"
     CDS             complement(457..>880)
                     /locus_tag="N7C74_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGU2434743.1"
                     /translation="SEVSLWKWGALSLSVPLILFLAVFFGGLKPEIHFPEWLPEFILA
                     NLFFVSLAEESLFRGYIQSRLSEVTSPLVALIVAALLFGFYHYSGGALLVLFATLSGV
                     VYGLSWMWSGRLWVATLFHFGLNLCHLLFFTYPFLKHN"
BASE COUNT          306 a          221 c          153 g          200 t
ORIGIN      
        1 gaactgatag tctgccagac ccgcataacc agctgggtca gtattaactg gcagcgctcg
       61 cgtgaaaggt acgtattctg cgcaatctcc ccgaccgtcg ccggttcggt gacgcttaat
      121 tcatcaaaca caactctggc ggtttctgtc atatcctgct gtttcagcat gtctttttac
      181 cctttccggt taacgtgaca caccaataac tcttgtcgaa aaagccagca agctgaaaga
      241 cctgtattaa taactaccag cacatttaac gcactgcgct actttgcggg cacaaaaaac
      301 ccgctcagag gcggggtcaa gctatgcggc gaaataacca ctcttaacag catacctgat
      361 tttttacgta cgtaaatgct ttgccgtgca tatttttcat gcaaatgtca cgtcctgcta
      421 tttttcagtc ttataaattt aaaaccatag aaaaaatcaa ttatgtttta aaaatggata
      481 ggtaaagaat aacaagtgac acagattcaa accaaaatgg aaaagggtgg caacccacaa
      541 acgcccactc cacatccatg acaatccata cacaacacca gataacgtgg caaataatac
      601 aagtaaagca ccacctgaat agtgataaaa accaaacaac aaagccgcca caattaatgc
      661 aaccaatgga gacgttactt ctgatagccg tgattgaata taccctctaa ataatgattc
      721 ctctgccaga gacacaaaaa acaaattagc caatataaac tctggcaacc actcaggaaa
      781 atgaatctct ggctttaatc caccaaaaaa aacagccaaa aacaggataa gaggaacaga
      841 gagcgacaga gccccccact tccacaaaga cacttctgat
//
LOCUS       DBFIFM010000483          880 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.494, whole genome shotgun sequence.
ACCESSION   DBFIFM010000483 DBFIFM010000000
VERSION     DBFIFM010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.494"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(1..216)
                     /locus_tag="N7C74_005089"
                     /pseudo
     CDS             complement(1..216)
                     /locus_tag="N7C74_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558227.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            310..573
                     /locus_tag="N7C74_005090"
                     /pseudo
     CDS             310..573
                     /locus_tag="N7C74_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            613..733
                     /locus_tag="N7C74_005091"
                     /pseudo
     CDS             613..733
                     /locus_tag="N7C74_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            730..880
                     /locus_tag="N7C74_005092"
                     /pseudo
     CDS             730..880
                     /locus_tag="N7C74_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          234 a          214 c          225 g          207 t
ORIGIN      
        1 tggctgtgtg cgcggtaaca gaccggactg aaattccagg aagcttttgc cctcagtccg
       61 tgacggcgca ggctttccgt cacggttttt actttaaaat ggtaaccctg ggcacgcagt
      121 tcatccatca ggcgttgtgc atgaccgggt aaaagcaacg aggacaacgc tgtcgcagtg
      181 ctgacggaac tgccgacgtg tacttatcct tatccgccag tgatataaga cggtaattca
      241 ccatttggat tgtccgctcc acccaacatg ttgtttcctt gaggttctca caccagaaag
      301 gacatcagca tgctgagcag agaggacttt tacatgataa agcaaatgcg ccagcaaggt
      361 gcgtatattg ttgatattgc gactcagatt ggttgctctg aacgaacggt cagacgttac
      421 ctgaaatacc cagaacctcc ggccagaaaa acacgccaca aaatggccga aatggactat
      481 atcgacatgc gtctggctga gaatgtctgg aacggcgaag ttatcctggc ggaaatcaaa
      541 gcgatgggtt ataccggtgg ccgttccatg ttgcttcgcc agttcaggaa gacaccggag
      601 cagcgcttta cgcaggaaca cagtctgttc ttccggttgc tgaaccgtcg atatgaaaaa
      661 gcgagcattg tgctgacgtc caataaaggg ttcgccgact gggggagaga tgttcggtga
      721 taacgtgctg gcaaaatatt cacccaacgc attgagcgta ataatctgac gctacgcacc
      781 cgcattaagc ggttgactcg taaaaccatc tgcttctcac gctcagttga gatccacgaa
      841 aaagtgatcg gagcgtttat cgaaaaacac atgttctact
//
LOCUS       DBFIFM010000484          873 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.495, whole genome shotgun sequence.
ACCESSION   DBFIFM010000484 DBFIFM010000000
VERSION     DBFIFM010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.495"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..90
                     /locus_tag="N7C74_005093"
     CDS             <1..90
                     /locus_tag="N7C74_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector protein"
                     /protein_id="HGU2434744.1"
                     /translation="VTFAAELFDTQNCNKKQNKKPWKKVMFNV"
     gene            83..>873
                     /locus_tag="N7C74_005094"
     CDS             83..>873
                     /locus_tag="N7C74_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HGU2434745.1"
                     /translation="MFNKVLVGLRSHPELIILGLMVMIIAMLIIPLPTYLIDFLIGLN
                     LTLAILVFLGSFYVDRILSFSSFPSILLITTLFRLALAISTSRLILLEADAGEIITSF
                     GEFVIGDSLVVGFVIFSIVTIVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLR
                     AGIIDADLAKERRSVLERESQLYGSFDGAMKFIKGDAIANIIIIFVNIIGGLSVGVGQ
                     NGMDFSTALTVYTILTVGDGLVSQIPALLIAISAGFIVTRVNGDS"
BASE COUNT          237 a          146 c          192 g          298 t
ORIGIN      
        1 gtaacatttg cagcagagct ctttgataca caaaactgta acaaaaaaca aaataaaaaa
       61 ccatggaaga aggtgatgtt caatgtttaa caaagttttg gtagggttaa ggagtcatcc
      121 agaattaata attcttggat taatggtgat gattatcgcc atgctcataa ttccattgcc
      181 cacctattta atcgactttt tgattgggct taacttaaca ttggcaatac ttgtattttt
      241 aggatcattc tatgttgata ggatcttaag cttttcttca tttccatcaa tcctccttat
      301 cacaacgtta tttcgtttgg cgctggcgat cagtaccagt cgacttatac ttcttgaggc
      361 ggatgctgga gaaattataa cgagttttgg tgagttcgtt attggagata gcctggtagt
      421 tggttttgtt attttctcca ttgttactat tgttcagttt atcgtgatta ctaaaggttc
      481 ggaacgtgtg gctgaagttg ctgcccgctt ttcgcttgac ggtatgccag gtaaacaaat
      541 gagcattgac gccgatctac gcgccgggat cattgatgca gatttagcaa aagaacgtcg
      601 tagtgtatta gaacgagaga gccagttgta tggttccttt gatggtgcga tgaaatttat
      661 caaaggtgat gctattgcaa acattattat tatctttgtt aatattatcg gtgggctatc
      721 agtaggagta ggccaaaatg gaatggattt ctcaacagcg ttgactgtct acacaattct
      781 tactgtcggt gatggtcttg tttctcaaat tccagcttta ttaattgcta ttagcgcagg
      841 atttatcgtt acgcgcgtaa atggcgatag tga
//
LOCUS       DBFIFM010000485          871 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.496, whole genome shotgun sequence.
ACCESSION   DBFIFM010000485 DBFIFM010000000
VERSION     DBFIFM010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.496"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..621
                     /gene="bet"
                     /locus_tag="N7C74_005095"
     CDS             <1..621
                     /gene="bet"
                     /locus_tag="N7C74_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HGU2434746.1"
                     /translation="LNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQ
                     DNESCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMI
                     QCARLAFGFAGIYNKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKT
                     WDDDLLPLCSQIFRRDIRASSELTQAEAVKVLGFLKQKASEQKVAA"
     gene            618..>871
                     /locus_tag="N7C74_005096"
     CDS             618..>871
                     /locus_tag="N7C74_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HGU2434747.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQY"
BASE COUNT          238 a          226 c          229 g          178 t
ORIGIN      
        1 cttaatccgt ggacgaaaga aatttacgcc ttccctgata agcagaacgg catcgttccg
       61 gtggtgggcg ttgatggctg gtcccgcatc atcaatgaaa accagcagtt tgatggcatg
      121 gactttgagc aggacaatga atcctgtaca tgccggattt accgcaaaga ccgtaatcat
      181 ccgatctgcg ttaccgagtg gatggatgaa tgccgccgcg aaccattcaa aacccgcgaa
      241 ggcagagaaa tcaccggccc gtggcagtcg catcccaaac ggatgttacg gcataaagcc
      301 atgattcagt gtgcccgtct ggccttcgga tttgctggta tctataacaa ggatgaagcc
      361 gagcgcattg tcgaaaatac tgcatacact gcagaacgcc agccggaacg cgacatcact
      421 ccggttaacg atgaaaccat gcaggagatt aacactctgc tgatcgccct ggataaaaca
      481 tgggatgacg acttattgcc gctctgttcc cagatatttc gccgcgacat tcgcgcatcg
      541 tcagaactga cacaggccga agcagtgaaa gttcttggat tcctgaaaca gaaagcctct
      601 gagcagaagg tggcagcatg acaccggaca ttatcctgca gcgtaccgga atcgacgtga
      661 gagctgtcga acagggggat gatgcatggc acaaattacg gctcggcgtc atcaccgctt
      721 cagaagttca caacgtgata gcaaagcccc gctcaggaaa gaagtggcct gacatgaaaa
      781 tgtcctactt ccacaccctg cttgccgagg tttgcaccgg tgtggctccg gaagttaacg
      841 ctaaagcact ggcctgggga aaacagtacg a
//
LOCUS       DBFIFM010000486          862 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.497, whole genome shotgun sequence.
ACCESSION   DBFIFM010000486 DBFIFM010000000
VERSION     DBFIFM010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.497"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            318..724
                     /gene="ipbA"
                     /locus_tag="N7C74_005097"
                     /gene_synonym="fimX"
                     /pseudo
     CDS             318..724
                     /gene="ipbA"
                     /locus_tag="N7C74_005097"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="IpbA is also known as HbiF and FimX; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
BASE COUNT          257 a          173 c          170 g          262 t
ORIGIN      
        1 gacgtaacac acatccccca cgccctgtaa ttcatagctt ttatatttat tttttcacat
       61 caatgtagat aaaaacacgc attaaaaatg gcttgtagaa aagcagcaca ataaatagtt
      121 catatttagt ctattgcgta gaactactat tcaaaaaatc ttattaatga gataatgaga
      181 ttacatcgat aacgttctga ttgcaggcat acttatctgg gtgtgcctct cttttttaca
      241 actgtacacg gtggcgtatg acgagaaaat atctcacaca ggatgaagtc tacaggctga
      301 tggatgcagc ccagagcatg tcctttcctg aaagaaatcg ctgtctaatt atgatggctt
      361 tcattcacgg ttttagggcc agtgaacttc ttgatttacg tttatccgat attgatgcat
      421 caggaaaaca acttaatatt cggcggatta aaaatggttt ctcgacaaca catcctcttc
      481 ttcctgatga atataatttg atcaagttgt ggcttaagca gaggaagtta attgaaaacg
      541 gagttgaagg agactggctt tttctctccc ggaaacgccg ccctatcagc agacaacatt
      601 tttttctatc attcgtgagg ctggaagacg tgcaggatta gctgtaaaag cacatcctca
      661 tatgttacgt catgcctgtg gttttgctct ggctgacaat ggtgtcgata cccgactact
      721 tcaggattat ctgggtcatc gaaatatcca gcatactgtc agatacacag ccagtaatgc
      781 tgctcgattt aaaggggtgt ggaaaaaaaa gcctcgttaa aacagtacct tctgattcaa
      841 aatgtcagat tcctacgttt tc
//
LOCUS       DBFIFM010000487          853 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.498, whole genome shotgun sequence.
ACCESSION   DBFIFM010000487 DBFIFM010000000
VERSION     DBFIFM010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.498"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            8..214
                     /locus_tag="N7C74_005098"
     CDS             8..214
                     /locus_tag="N7C74_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HGU2434748.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
     gene            279..503
                     /locus_tag="N7C74_005099"
     CDS             279..503
                     /locus_tag="N7C74_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434749.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
BASE COUNT          210 a          189 c          220 g          234 t
ORIGIN      
        1 ttagccgatg cgagagctga aaatgaaact ctgcgtgctg atgttgccgc tggtcgtaag
       61 cgcctgcgga tcaacgccac ctgccccggt accgtgcgtg aagccaccgg cacctccggc
      121 gtgggcaatg atgccgccgt cgaactctct ccggttgctg gacgaaacgt tctcggtatc
      181 agagacggaa tcatcagcga ccaggcagca ttgagaatgc ttcaggaata tatccgcact
      241 cagtgtatta actagtattt ttgttattcg gagaatgcat gaagaaatta ttggtaaccg
      301 taaagccttt tcagggaaca attccgttcc gtattttgca gcgtggtcgt gttcttgttg
      361 aaggttcgtt cagtggtaaa tgtacgcaat tacactccag gacctttcag gtgaatgcca
      421 cgaatgaaga gctaaccgtt gagtgtacga tgaatgccgc taaatgccgc atggtatccg
      481 ctgcattaca gccagtgtgt tgagcgacct tattattcat gcgcggtatt gtcgccgtat
      541 tcctgcatta acagagaccg cagcccgacc gggagaatcc tctgcgcgag tgtgcgggga
      601 taatcaaaaa cgatacacac cggggtttac cgcgttaacg gagcgcggcg ttgtcccctc
      661 atggtcgctg gtccggtgcg atggtggaag aagccggatg tttatcacta ttaattgata
      721 acacagaaat ggattcattg attttcagca cgtttttgta ttcgtattat tgaacatctg
      781 tttattttac ttttaacata ttgataataa aaagagctgt aaatctttag atgagtcgat
      841 tttgtccggg gaa
//
LOCUS       DBFIFM010000488          850 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.499, whole genome shotgun sequence.
ACCESSION   DBFIFM010000488 DBFIFM010000000
VERSION     DBFIFM010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.499"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(100..771)
                     /gene="queE"
                     /locus_tag="N7C74_005100"
     CDS             complement(100..771)
                     /gene="queE"
                     /locus_tag="N7C74_005100"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HGU2434750.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          218 a          217 c          190 g          225 t
ORIGIN      
        1 ggataggcaa gatggatgag ttttcagaga cgacaaagaa tataaatgag tagaagtctt
       61 tacaggttcc acccatttat aacgcttata aatgtttaat caggcaatat ttaaatattt
      121 atgggtttgc atcgacaaac gccaattacg cgcaatgcag gtttcaatgc acaaacgtgt
      181 ggcatcatcc ttttggctaa tcggctgcag tgcaatgact cgcggtttat catcggtcag
      241 cgtcgccaaa agttcatcca gtgcttcaat atcacgtacg cgtcccactg gatgcttgat
      301 ttcgttggct cgctccagtg cctgtgacaa cacttcatag ccgccgcgca tgttcagctt
      361 tggcgatacg gtaacccagg tattcggtgt acagcgtact tcatgagtac cactggtttc
      421 gatctggcag ctaaaaccgt tcttttcgag caggtcagtc agtggcagca aatcatgaat
      481 gcaaggctca ccacccgtaa tcaccacatg ccgcgcggta tatccctggc gaccaatgac
      541 agccagcaag tcttcactgc tcgccgcccc ccacttatca ctctctttgg tcttcgccag
      601 aatgctgaaa agggagactt cccgatcctc aagcttttcc caggtgtgtt tggtgtcgca
      661 ccaggcacag ccaaccgggc atccctgtaa acgaataaaa atggcgggga cgccggtaaa
      721 gtaaccctca ccttgcaggg tctggaacat ctcgttaatc gggtactgca tagcattctc
      781 tgtgaagtgg ataattgtta attattgcag atcctgccac aacaatcatg tcttattaac
      841 attctgttac
//
LOCUS       DBFIFM010000489          846 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.500, whole genome shotgun sequence.
ACCESSION   DBFIFM010000489 DBFIFM010000000
VERSION     DBFIFM010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.500"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..243
                     /locus_tag="N7C74_005101"
     CDS             <1..243
                     /locus_tag="N7C74_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="may be involved in the export or assemby of
                     proteins involved in the entry of Salmonella into host
                     cells; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscC/YscC/HrcC family type III secretion system
                     outer membrane ring protein"
                     /protein_id="HGU2434751.1"
                     /translation="LGSIPFIGKLFGYEGTNANNIVRVFLIEPREIDERMMNNANEAA
                     VDARAITQQMAKNKEINDELLQKWIKTYLNREVVGG"
     gene            248..>846
                     /locus_tag="N7C74_005102"
     CDS             248..>846
                     /locus_tag="N7C74_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0046903 - secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HrpJ domain-containing protein"
                     /protein_id="HGU2434752.1"
                     /translation="MAIHVEHVGVLERAREVSRLEDIITEDNEDIEAEMPKMRDDPAG
                     KEARFLQATDEMSAALTQFMKKKIYEEQLANFLDGEEYVLEDQPIEKTDKVMEALKAA
                     TTHDYEVYSFAKKLFPDESDLVVVLRAILRKKQISENVRLNAEALLRKVNQETTKKFI
                     NSGINSALKAKLFGQALSLNPKLLRASYRQFLMAEDDAV"
BASE COUNT          307 a          135 c          195 g          209 t
ORIGIN      
        1 ttaggcagca ttccatttat tggtaaatta tttggttatg aaggaacaaa tgcgaataac
       61 atcgttcgtg tctttcttat tgagccaaga gagatcgatg aacgcatgat gaataatgcg
      121 aatgaggctg cggttgatgc cagagcgatt acacagcaaa tggcaaaaaa taaagaaatc
      181 aacgatgaat tactgcagaa atggataaaa acttacctga atcgtgaagt cgtcggagga
      241 tagtgaaatg gcgattcatg tagagcatgt cggtgtatta gaaagagcca gggaagtttc
      301 tcgcttagag gacattatca cagaagataa tgaagacatt gaagctgaaa tgcctaaaat
      361 gagggatgat cccgcaggta aagaagctcg gttcttacaa gctactgacg agatgtcagc
      421 agcgttaacg cagtttatga agaaaaaaat ttacgaagaa caactggcca attttctgga
      481 cggagaagaa tatgtcctgg aagaccagcc aatagaaaaa accgacaaag ttatggaggc
      541 gcttaaggct gcaactaccc atgactatga agtgtatagc tttgctaaaa aattattccc
      601 agatgaaagc gatctggtag ttgtgttacg tgcaatttta cgtaaaaaac aaatatcaga
      661 aaatgtccgg ctaaacgctg aagcattact tagaaaagtt aatcaagaga ctactaaaaa
      721 attcattaat agcggaatta actcggcatt aaaagcaaag ttatttggcc aggcgctgtc
      781 attaaatcca aaattactaa gggcgagtta tcggcaattt ttaatggcag aagacgatgc
      841 tgtaga
//
LOCUS       DBFIFM010000490          827 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.501, whole genome shotgun sequence.
ACCESSION   DBFIFM010000490 DBFIFM010000000
VERSION     DBFIFM010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.501"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..>827
                     /locus_tag="N7C74_005103"
     CDS             <1..>827
                     /locus_tag="N7C74_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU2434753.1"
                     /translation="REKIGKLHLAQGLWELIDNSQLADEMAEMKTTITETRNEITQTV
                     SKTLENQSATIQQIQRVQKDTNDDLAALYMLKVQKTKDGIPYVAGIGAGIEDTDGQPL
                     SNILLLADRIAMINPESGNSTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPTFSLT
                     PDGRLTAKNADISGSVNANSGTLNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRT
                     STYASGTITVRISDDQKFDRQVMIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNG
                     "
BASE COUNT          239 a          197 c          232 g          159 t
ORIGIN      
        1 cgggaaaaaa taggaaaact gcatctggct caggggctat gggagctgat agacaacagc
       61 cagcttgcgg atgagatggc ggagatgaag accaccatca cggaaacccg caatgaaatc
      121 acacagacgg tcagtaaaac gctggagaac cagagcgcca ctatacagca gatacagcgc
      181 gtgcagaagg acacaaatga tgacctggct gcgctgtaca tgctgaaggt tcaaaaaacg
      241 aaagacggca ttccctatgt ggccgggatt ggtgcaggga ttgaggatac tgatggccag
      301 ccactgagca acatactgct gctggctgac cgtatcgcga tgataaatcc ggagagcggc
      361 aacagcacgc cgttatttgt ggcgcagggg aatcagctgt tcatgaacga cgtgttcctg
      421 aagcgactgt ttgcggtgag tatcacctcg tccggcaatc ccccgacgtt ttccctgacg
      481 ccggacgggc gactgacggc gaaaaatgcg gatatcagtg gcagtgtgaa tgcgaactca
      541 gggacgctca acaacgtcac gattaatgag aactgtcaga ttaaggggaa actgtcagcc
      601 aaccagattg aaggcgatat tgtcaaaacg gtcagcaagt ctttcccccg cacgagcact
      661 tatgccagtg gcaccatcac ggtaagaatc agtgatgatc agaagtttga ccggcaggtc
      721 atgataccgc cagtgttatt ccgcggtggt aagcatgaga atttcaacag taataaccaa
      781 cagtcatact ggtattcaac ctgccggtta agagtgaccc gcaatgg
//
LOCUS       DBFIFM010000491          825 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.502, whole genome shotgun sequence.
ACCESSION   DBFIFM010000491 DBFIFM010000000
VERSION     DBFIFM010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.502"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..268
                     /locus_tag="N7C74_005104"
     CDS             <1..268
                     /locus_tag="N7C74_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434754.1"
                     /translation="TRAYFGELQASIEQDINQPVRQEIDAFFCAFREKVEQLRNTLIQ
                     SSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            265..>825
                     /locus_tag="N7C74_005105"
     CDS             265..>825
                     /locus_tag="N7C74_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HGU2434755.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGA"
BASE COUNT          203 a          208 c          233 g          181 t
ORIGIN      
        1 gacccgggca tatttcgggg agctgcaggc atccattgag caggatatta accagcccgt
       61 ccgccaggaa atcgatgcct ttttctgcgc attcagggag aaagttgaac aactgcgtaa
      121 cacgctgatt cagagctctg aagatcataa acgcgatcag caggcgcagg aacggcttac
      181 cgggcgactt caggcattaa atgaaagggt tcctgagctc attactgaca gtaaggcgct
      241 gagggaagag ctggagacaa tgctgtgact tcaccattta ttcaacaaat tgctgataac
      301 cgggtatgtc aggtactcac ctgtcttcct gaaaaatttg tggttgattt cgcaaacgga
      361 atcgatgttg cacaggagca tatccgcacg gccggagagc gcacgttctt ccggcgttta
      421 aaagaggggc tgaccggcga aggcgctgca cggcagaatg ccatcaatgc gtcgcttgca
      481 cagggcgttg aggcgtccct gcgctggctg acggagatga caacgtcact tgccaccaca
      541 aactacgcga ttacccgggt aaacgacagg gtcagttcac tggtcagtga tacagccagg
      601 ctggcgcatt attccgcaga tacgcgggag cagttactca ccctggccga tcaggttcac
      661 cataaactga atcatcttga agaaaaactc caccgtgttg accaggttca gcgggcgcag
      721 ttacatcttg agcagatatt ctcatggtgg agcgccgggc gatatgcgtc attttcccct
      781 gccggacgtt gttatgtggc acttgaagag cttcgctggg gcgcg
//
LOCUS       DBFIFM010000492          819 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.503, whole genome shotgun sequence.
ACCESSION   DBFIFM010000492 DBFIFM010000000
VERSION     DBFIFM010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.503"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..355
                     /locus_tag="N7C74_005106"
     CDS             <1..355
                     /locus_tag="N7C74_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU2434756.1"
                     /translation="EGDTNVAVVMGCKSAKDRTISIVLGNSMLQTLFEKRLADDGEIE
                     NLFDQQGYFSCDSLTAEEVMMLKDLFDIRVLHLSNKFNVGLQGNINTDVLQDSFFKNV
                     DFIHESNSYTVGMGV"
BASE COUNT          242 a          153 c          197 g          227 t
ORIGIN      
        1 tgagggggat acgaatgttg ccgttgtgat ggggtgtaaa tctgcgaaag atcgcacgat
       61 atccattgtc cttggcaact cgatgttgca aacactcttt gaaaaacgcc tcgccgatga
      121 tggagaaata gaaaaccttt ttgatcaaca gggatatttt agttgcgatt cattaacagc
      181 cgaagaagta atgatgttaa aagacctttt tgatattcga gttcttcatc tctctaacaa
      241 atttaatgtt gggttgcaag gtaatatcaa cacggatgtt ttacaagatt cgttttttaa
      301 aaatgtcgat ttcattcatg aatctaactc gtataccgta gggatgggag tctgaagagt
      361 aaaactcatt aggattatca gaattcggcc aatgcattgc aaaaatgaaa tacaaagaga
      421 gatgtgtgca ggcgcttagc cgcgaggggc atggcgaagc catacagaaa gcaccggcat
      481 tcagaactcc tgtgaaagcg gcatagtgcc gctgctcagg agttttgtta tgacccgtat
      541 cgtgctgaac gttgccaccc tatgatgcga ttaaaaaaac tcaccgtcga gccgatgatt
      601 gacgtcaatg ctatcggatg atgccggggg tgttccggaa ggaattcctt gtttcccgaa
      661 ctgttaccaa caaaattata acgatgagca agtaaagacc ggttggtggc agtactgacc
      721 ggtctgtgat ggaagattaa tttcacctta aatcctggtg ctgttaggat gctgacgttt
      781 ttcgggtaca ccattcagat tatgcaaata aagattcaa
//
LOCUS       DBFIFM010000493          818 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.504, whole genome shotgun sequence.
ACCESSION   DBFIFM010000493 DBFIFM010000000
VERSION     DBFIFM010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.504"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            98..808
                     /locus_tag="N7C74_005107"
     CDS             98..808
                     /locus_tag="N7C74_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU2434757.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSNLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
BASE COUNT          232 a          207 c          170 g          209 t
ORIGIN      
        1 gaaacccggt gactcgccaa tgtattagca acatttaccg attgtctgat gtaacggttt
       61 tcccgcttat cctcttaaca catggagaat gcatcagatg caaactacgc gtacccccta
      121 ctctatttcc tttatggcga cagtgttact tttattgctc tttgcttgcc atagcaccgt
      181 tgccaacgca gctgtcgcac tgggtgcgac gcgggtaatt tatccggcaa accagaaaca
      241 ggttttattg cctgtcacga ataatgatcc tgccagtgtc tatctcattc agtcatggat
      301 agaaaatgct ggtgaccaaa aagacaccca gtttgtcatt accccacctc ttttttccat
      361 gcaaggtaaa aaagagaata cgctgcggat aatcaacgcc actaatcatc agttacccgg
      421 cgaccgtgaa agcctgttct gggttaatgt taaagctatt cctgctatgg aaaaagacca
      481 gaagaatgag aatacattac agctggcaat tattagccgc ataaaaatgt tttatcggcc
      541 cacgaacctg gcaatggcac ctgaagaagc gcctgcaatg cttcggtttc gtcgcagcgg
      601 tagcaatctc actcttatta atccaacgcc ctacttcatt accgtaacaa atatgaaagc
      661 aggaaatagc aatctcccaa acaccatggt gccgccaaaa ggtgaagtgt cggtggatat
      721 cccgcatgct gccaccgggg atatcagttt tcagacgatc aatgattacg gcgcattaac
      781 tccgcgtatt aaggcaacca tgcagtaagc cagtgacc
//
LOCUS       DBFIFM010000494          816 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.505, whole genome shotgun sequence.
ACCESSION   DBFIFM010000494 DBFIFM010000000
VERSION     DBFIFM010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.505"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..>816
                     /locus_tag="N7C74_005108"
     CDS             <1..>816
                     /locus_tag="N7C74_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434758.1"
                     /translation="QIFPLHDEIGFNFKDLPGPLKNALSNSNISTTAVSTIASTGTSA
                     TTSTVTTEPKDPIPWFGLTAQVVRNHGVELPIVKTENGWKLVGETPLTPDGPKANYTE
                     EWVIRPGEADFKYGASPLQATLGLEFGAHFKWDLDNPNTKYAVLTNAAANALGALGGF
                     AVSRFASTDPMLSPHIGAMVGQAAGHAIQYNTPGLKPDTILWWAGATLGAADLNKAEF
                     EVARFTDYPRIWWHAREGAIFPNKADIEHATGADIRAMEEGIPVGQRHPNPEDV"
BASE COUNT          242 a          171 c          196 g          207 t
ORIGIN      
        1 cagatattcc ctctgcatga tgaaataggt tttaatttta aagaccttcc tggcccgtta
       61 aaaaatgcat taagcaacag taatatatca accactgcag tgtcgactat tgcatcgact
      121 ggaacatcag ccactacttc gacggtaacc accgagccaa aagacccaat accatggttt
      181 ggattaacag ctcaagtggt tcgtaatcat ggtgtagaac ttcctatagt caaaactgaa
      241 aatggatgga agcttgttgg agaaacacca cttactcctg atgggccgaa agcaaattac
      301 acggaggagt gggttatcag accgggagaa gcagatttta aatatggtgc atctccatta
      361 caggcaactc tagggctgga gtttggcgca catttcaagt gggatttaga taaccctaat
      421 actaaatatg ccgttcttac caatgctgcc gcaaatgcgc ttggtgcttt agggggattt
      481 gcagtatcca gatttgctag tacagatcca atgttaagtc ctcatatcgg tgcaatggtt
      541 gggcaagcag cagggcatgc tatacagtat aatacccctg gattaaagcc agacactatt
      601 ttatggtggg ctggtgcgac actgggggct gccgatttaa acaaggccga gtttgaagta
      661 gctagattca ctgactatcc tcgtatatgg tggcacgcaa gagaaggagc tattttcccc
      721 aataaagcag atattgaaca tgccacaggt gctgatatac gcgcaatgga agaaggtatc
      781 cctgttggac agcggcatcc aaatccagag gatgtg
//
LOCUS       DBFIFM010000495          815 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.506, whole genome shotgun sequence.
ACCESSION   DBFIFM010000495 DBFIFM010000000
VERSION     DBFIFM010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.506"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..>815
                     /locus_tag="N7C74_005109"
     CDS             <1..>815
                     /locus_tag="N7C74_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU2434759.1"
                     /translation="EDRRVALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQ
                     QRQGLVNVPLRLPQATLDDKQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVG
                     DPYQEARQRYISNALEAWRNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQ
                     NTELAKLKYQTALGELKTLSEIQKQELLRNAALIDQQKIREQLRYREETLKNDNVAAR
                     ASNESELLGYGQGERARERMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQA"
BASE COUNT          224 a          185 c          266 g          140 t
ORIGIN      
        1 gaagaccgtc gtgtggcgtt aattcgtcag caggcggcag agcagaataa ggtgtaccag
       61 tccatgctgg ttatgaacgg tcagcatacg gaattcaacc gtctgctggg gctggggaat
      121 gaactgcttc agcagcgtca gggactggtg aatgtaccgt tacggctgcc gcaggccacc
      181 ctggatgata aacagcagag tgccctgaca aaaacagagc gtgagctggc cctgtccaga
      241 ctgaaagggg aagaaaaaga gcgtgtccga ctggggtatg cggcggatga cctcggtttt
      301 gtgggtgatc cgtatcagga ggcgagacaa cgttatatca gtaatgccct ggaagcctgg
      361 cgcaataacg aggcgaataa acccaaatcc cggggtggaa aatcagagac ggaaaaagcg
      421 gaagacagtt tttcccggct gctgaagcag cagaaagagc aactggcact ggccggtcag
      481 aacacggagc tggcgaagct gaagtaccag acagcgctgg gtgaactgaa aaccctgtcg
      541 gagatacaga agcaggaact gctgcgcaat gcggccctga ttgaccagca aaaaatccgg
      601 gagcagttgc ggtaccggga agagaccctg aagaatgata atgtggctgc gcgtgcatca
      661 aatgaatctg aactgctggg gtacgggcag ggggagcgag ccagggaacg catgcgggag
      721 ttgcagcaga tccgcgacag cttccgccag aaggatgcgg accttcagtc tcagtatcag
      781 accggggata tcagtgagga tttttacaga caggc
//
LOCUS       DBFIFM010000496          815 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.507, whole genome shotgun sequence.
ACCESSION   DBFIFM010000496 DBFIFM010000000
VERSION     DBFIFM010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.507"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..>815
                     /locus_tag="N7C74_005110"
     CDS             <1..>815
                     /locus_tag="N7C74_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU2434760.1"
                     /translation="EDRRVALIRQQAAEQNKVYQSMLVMNGQYTEFNRLLGLGNELLQ
                     QRQGLVNVPLRLPQATLDDKQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVG
                     DPYQEARQRYISNALEAWRNNEVNKPKSRGGKSETEKAEDSFSRLLKQQKEQLALVGQ
                     NTELAKLKYQTALGELKTLTEMQKQELLRNATLIDQQKIREQLRSREETLKNENAAAR
                     ASNDAELLGYGQGERARERMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQA"
BASE COUNT          227 a          181 c          269 g          138 t
ORIGIN      
        1 gaagaccgtc gtgtggcgtt aattcgtcag caggcggcag agcagaataa ggtgtaccag
       61 tccatgctgg ttatgaacgg tcagtatacg gaattcaacc gtctgctggg gctggggaat
      121 gaactgcttc agcagcggca gggactggtg aatgtgccgt tacggctgcc acaggccact
      181 ctggatgata aacagcagag tgccctgaca aaaacagagc gtgagctggc cctgtccaga
      241 ctgaaagggg aagaaaaaga gcgcgtccga ctggggtatg cggcggatga cctcggtttt
      301 gtgggggatc cgtatcagga ggcgagacaa cgttatatca gtaatgccct ggaagcctgg
      361 cgcaataacg aggtgaataa acccaaatcc cggggtggaa aatcagagac ggaaaaagcg
      421 gaagacagtt tttcccggct gctgaagcag cagaaagagc aactggcact ggtggggcag
      481 aatacagagc tggcgaagct gaaataccag acagcgctgg gtgaactgaa aaccctgacg
      541 gagatgcaga agcaggaact gctgcgtaac gcgaccctga ttgaccagca aaaaatccgg
      601 gaacagttgc gatcccggga agagacactg aagaatgaga atgcggctgc gcgtgcgtcg
      661 aatgatgctg aactgctggg gtacgggcag ggggagcgag ccagagaacg catgcgggag
      721 ttgcagcaga tccgcgacag cttccgccag aaggatgcgg accttcagtc tcagtatcag
      781 accggggata tcagtgagga tttttacaga caggc
//
LOCUS       DBFIFM010000497          811 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.508, whole genome shotgun sequence.
ACCESSION   DBFIFM010000497 DBFIFM010000000
VERSION     DBFIFM010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.508"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..82)
                     /locus_tag="N7C74_005111"
     CDS             complement(<1..82)
                     /locus_tag="N7C74_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434761.1"
                     /translation="MPKISSVVSSCYHLFSEHQQLSNETTM"
     gene            complement(161..811)
                     /locus_tag="N7C74_005112"
     CDS             complement(161..811)
                     /locus_tag="N7C74_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HGU2434762.1"
                     /translation="MPVILNFSNGSVLPENELEALRHIARSNQNDTITIGGRNMRLHY
                     IQFMDGFSVEPILGGLWDHLGAREAHHLADRLTRQLNGGNTFLQAYSLYLEQRQAAPL
                     VQESVIKTLLDRINSNAFPVSLQDFSCTEEHLNCPITLHIPETGVFVRNARNSEICAL
                     YDQEALTELILRNALHPLSRDPFAPEMIISKDKCHFNITKQCFYALPIYPLQQNSI"
BASE COUNT          227 a          148 c          166 g          270 t
ORIGIN      
        1 tcattgttgt ttcatttgaa agttgttgat gttcactgaa cagatggtaa catgatgata
       61 caactgatga tattttgggc ataaatagtc tcctcgctat atgtttgtta tatggtgatt
      121 aacttattga aaatatgctt cttatattgt gcttttattt ttaaatactg ttttgttgaa
      181 gtgggtatat gggtaatgcg taaaaacatt gttttgttat attaaaatga cacttgtctt
      241 tgcttataat catttctgga gcaaaggggt cacggctgag ggggtggaga gcgttacgca
      301 ggataagttc agtcaatgct tcctgatcat ataatgcaca tatttctgaa tttcgagcat
      361 ttctgacaaa aacacctgtc tcaggaatat gtagcgttat cggacaatta agatgttcct
      421 cagtacagga aaagtcttgt aaactaacgg gaaatgcatt cgaatttatt cgatctagta
      481 gtgtttttat gacgctctcc tgcacaagcg gggcagcttg cctctgctcc agatataagc
      541 tgtatgcctg cagaaaagtg ttacctccgt taagttgtct tgtcaggcgg tctgcgagat
      601 gatgagcctc tcttgcccca agatggtccc aaagtccacc aagaataggc tcaacactaa
      661 aaccatccat aaactggata taatgaagcc tcatattacg gcctcctata gtaatagtgt
      721 cattctggtt gcttcttgct atatgtcgta aagcctccag ctcattttcc ggcaatacac
      781 ttccattaga aaaatttaat atgactggca t
//
LOCUS       DBFIFM010000498          806 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.510, whole genome shotgun sequence.
ACCESSION   DBFIFM010000498 DBFIFM010000000
VERSION     DBFIFM010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.510"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..625
                     /locus_tag="N7C74_005113"
     CDS             <1..625
                     /locus_tag="N7C74_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HGU2434763.1"
                     /translation="DFDKRKMEFTYPGANKSHLQSSNSDVVAAAAISITATEMKTILP
                     DDLTLGKYNKIYLSGHGSAGLPLLKCGDEFLSPSDIVDRIVQHNLHEIDDIRLTSCNS
                     ANIIKNKDFSPDEIEKSANMNNGWLARALFGQKRSLAEHVYAEFERRGINVSISGYHG
                     TGVFYVPEHGKPTTHLRSTTVPATPEHTVRRSDYRATLGRTQPIDIE"
BASE COUNT          258 a          165 c          169 g          214 t
ORIGIN      
        1 agattttgat aaaaggaaaa tggagtttac atatcctgga gcaaataaaa gccatctaca
       61 atcaagtaat agcgatgttg ttgctgctgc agctataagt attacagcga cagagatgaa
      121 aaccatcctg ccagatgatt taacactagg aaaatacaac aaaatttatc tgtctgggca
      181 tggttctgct ggtctacctc ttcttaagtg cggagatgaa tttttatcac cgtcagatat
      241 tgtcgaccgc attgttcagc ataatcttca tgaaatagat gatatcagat taacatcctg
      301 taactcagcc aacataataa aaaacaaaga cttctctcct gatgaaatag aaaaatccgc
      361 aaatatgaat aacggctggt tggccagggc attatttggt caaaagaggt ctttagcaga
      421 acacgtctat gccgagtttg aacgtcgcgg aattaacgtt tctatatcag gttaccatgg
      481 cactggcgtt ttttatgtac cagagcatgg taaaccaaca acgcatctac gctccacaac
      541 tgtgcctgca acacctgaac atactgtaag aagaagcgac tacagagcca ctttgggtag
      601 aactcaaccc atagatattg agtaattgag ccagcatata cacttgatgg ttaggaaaca
      661 gaatcattgt tttaaacatt tcctggccat taatattgtc ggcgggagca tatccaggac
      721 tggccggcaa accggtacgc gatctgttgc catggaaagt tgatctgagc gctcagtaaa
      781 tatcaatacg gttctggcga gccgct
//
LOCUS       DBFIFM010000499          792 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.511, whole genome shotgun sequence.
ACCESSION   DBFIFM010000499 DBFIFM010000000
VERSION     DBFIFM010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.511"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..>792
                     /locus_tag="N7C74_005114"
     rRNA            <1..>792
                     /locus_tag="N7C74_005114"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          198 a          181 c          262 g          151 t
ORIGIN      
        1 aagaccaaag agggggacct tcgggcctct tgccatcgga tgtgcccaga tgggattagc
       61 tagtaggtgg ggtaacggct cacctaggcg acgatcccta gctggtctga gaggatgacc
      121 agccacactg gaactgagac acggtccaga ctcctacggg aggcagcagt ggggaatatt
      181 gcacaatggg cgcaagcctg atgcagccat gccgcgtgta tgaagaaggc cttcgggttg
      241 taaagtactt tcagcgggga ggaagggagt aaagttaata cctttgctca ttgacgttac
      301 ccgcagaaga agcaccggct aactccgtgc cagcagccgc ggtaatacgg agggtgcaag
      361 cgttaatcgg aattactggg cgtaaagcgc acgcaggcgg tttgttaagt cagatgtgaa
      421 atccccgggc tcaacctggg aactgcatct gatactggca agcttgagtc tcgtagaggg
      481 gggtagaatt ccaggtgtag cggtgaaatg cgtagagatc tggaggaata ccggtggcga
      541 aggcggcccc ctggacgaag actgacgctc aggtgcgaaa gcgtggggag caaacaggat
      601 tagataccct ggtagtccac gccgtaaacg atgtcgactt ggaggttgtg cccttgaggc
      661 gtggcttccg gagctaacgc gttaagtcga ccgcctgggg agtacggccg caaggttaaa
      721 actcaaatga attgacgggg gcccgcacaa gcggtggagc atgtggttta attcgatgca
      781 acgcgaagaa cc
//
LOCUS       DBFIFM010000500          792 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.512, whole genome shotgun sequence.
ACCESSION   DBFIFM010000500 DBFIFM010000000
VERSION     DBFIFM010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.512"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..>792
                     /gene="ehxD"
                     /locus_tag="N7C74_005115"
     CDS             <1..>792
                     /gene="ehxD"
                     /locus_tag="N7C74_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HGU2434764.1"
                     /translation="ASLLYEKTEQYRYAILSESIQRNELPEIRITDFPGGEDNAGGEH
                     FQRVSSLIKEQFMTWQNRKNQKQITLNKKIVERDAALARVSLYEHQVSQEGRKLNDFK
                     YLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQVSQLEKEIELVREELALETNIFRSE
                     IIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAPVSGTVQELNIHTEGGVVTTAETLM
                     IIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVDAYPYTRHGYLTG"
BASE COUNT          289 a          126 c          173 g          204 t
ORIGIN      
        1 agcatctttg ttgtatgaaa aaacagaaca atatcgctac gcaattcttt ctgaatcaat
       61 acaacggaat gagcttcctg aaataaggat aacagatttt cctggcggag aagataatgc
      121 gggaggtgaa cattttcaga gggttagctc attaataaaa gagcaattca tgacatggca
      181 gaacagaaaa aatcaaaagc agatcacatt aaataaaaaa atagttgaac gggatgcagc
      241 gcttgctcgt gttagcctgt atgagcatca ggtatcacag gaaggaagaa aactcaatga
      301 ttttaagtat ttgttgaata aaaaagctgt ttctcaacat tcagttatgg agcaggaaaa
      361 tagctatatt caggcaaaaa atgaacatgc agtctggctt gcacaggttt ctcaacttga
      421 aaaagaaata gaacttgtgc gggaagaact ggcactggag acgaatatct tcagaagtga
      481 aattatcgag aagcacagaa aatcaacaga taacattgtg ttgctggagc atgagcttga
      541 aaaaaacagg cagagaaaag catcatcttt tattaaagct cctgtgagtg gtactgttca
      601 ggagttaaat atacatacag aaggtggtgt tgtcacaaca gcagaaacgc tgatgattat
      661 tgtccctgat aatgatattc tcgaagtaac agcatctgta cttaacaagg atatcggttt
      721 tatacaacct ggacaggagg tcgttattaa agtagatgca tatccatata cacgtcatgg
      781 ttaccttaca gg
//
LOCUS       DBFIFM010000501          780 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.513, whole genome shotgun sequence.
ACCESSION   DBFIFM010000501 DBFIFM010000000
VERSION     DBFIFM010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.513"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..527)
                     /gene="fimE"
                     /locus_tag="N7C74_005116"
     CDS             complement(<1..527)
                     /gene="fimE"
                     /locus_tag="N7C74_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HGU2434765.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNA"
BASE COUNT          252 a          179 c          152 g          197 t
ORIGIN      
        1 gcattactgg cggtataacg tacagtatgg cgaatatttc gatgcccgag ataatcctga
       61 attaaacgag tatctgcacc acgctccgcc agttcataac cgcaagcatg ccttaacata
      121 tgaggatgag tctgcgttac ggttccagct tcaataccgg catcacgaat aatgcgatag
      181 gcctgctggc gagaaagccg actcccgcgg cgagaaataa atatggcgtc ggtccggtca
      241 gcgcctttcc agttagcacg ttctagggtc cagcgttcca cagcttcacg ctcatcaaaa
      301 cgtaacgggt gaacggtaga aaatccgttc ttcagtcggc gaatatttat tcggccttca
      361 ttaaggtcaa gatcctgata atgcagatca agcagttcac taatacgcat cccatgccga
      421 tatgccaaca gaataagaca ataatctctg gctcccgttg ccccgtaaca aaccgcctgc
      481 atcatggcct gaacttcttt accggtaaga taacgacgtt tactcacaat agtagtactc
      541 ctaactgaga tatatttgaa tgaataccta taggaaacct caatcggtca aaattagccc
      601 aatagataaa aagataacca cacacgaagt gatgtggcta tcagtcaatt acattaaata
      661 tcatacaaaa ataaaatatc actgatgtga taacatctat tttcattgat ttgattcaat
      721 ataacaatag attgcagtgg attaacttcc agttgcttaa aaaataaagg aaaaagtgca
//
LOCUS       DBFIFM010000502          779 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.514, whole genome shotgun sequence.
ACCESSION   DBFIFM010000502 DBFIFM010000000
VERSION     DBFIFM010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.514"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..>779
                     /locus_tag="N7C74_005117"
     CDS             <1..>779
                     /locus_tag="N7C74_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGU2434766.1"
                     /translation="MNVTFDNLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMAT
                     FDGHIVEALMKMPEVNAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGER
                     KEAGKYTSVVDLARARANQQHTENSTGKINPVIAAIHREYKQTWKTLDDELAYALWPG
                     DVDAGNIDGSIHRWAKNEVIDNGREDWKRISASMRKQPDALRYDRQTIFGLVRERPID
                     IHKDPVALNKYITEYLTTKGVFEDEGTNQSATDTLSSPVPE"
BASE COUNT          236 a          190 c          193 g          160 t
ORIGIN      
        1 cgatgaacgt cacttttgac aacctggccc ctgaagtaca gaacgctgtc atggttaagt
       61 tcgacacatg tgaaaacatc actgttgata tggtgattag cgcacaggaa ttgttgcagg
      121 aagacatggc aacattcgac ggccatatcg ttgaagcgtt gatgaaaatg ccagaagtta
      181 acgccatgta tccggagctt aagctgcatg ccatcgggtg ggttaagcat aaatgtaagc
      241 ctggtgccaa atggcccgaa attcaggcag agatgcgcat ctggaaaaaa cgtcgcgaag
      301 gtgaacgcaa ggaagccgga aaatacacgt ctgttgttga tctcgcccgc gccagagcca
      361 atcaacagca cactgaaaat tcaacaggaa aaatcaaccc ggtcattgct gccattcatc
      421 gcgaatacaa gcagacatgg aaaacactgg atgacgaact ggcctacgct ctctggcctg
      481 gtgatgtgga tgccggaaac attgacggca gcatccatcg ctgggcaaaa aatgaagtta
      541 tcgacaacgg ccgcgaagac tggaagcgta tctcggcatc aatgcgcaaa cagcctgatg
      601 cccttcgcta cgaccgccag actatttttg gccttgtccg tgaacgtccg atcgacattc
      661 acaaagaccc tgtggcactg aacaaataca ttactgaata cctgactaca aagggcgtgt
      721 ttgaagatga aggaacaaat cagagcgcaa ctgatactct ctcgtcgcca gtaccagaa
//
LOCUS       DBFIFM010000503          771 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.515, whole genome shotgun sequence.
ACCESSION   DBFIFM010000503 DBFIFM010000000
VERSION     DBFIFM010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.515"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..226)
                     /gene="nleE"
                     /locus_tag="N7C74_005118"
     CDS             complement(<1..226)
                     /gene="nleE"
                     /locus_tag="N7C74_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase NleE"
                     /protein_id="HGU2434767.1"
                     /translation="MINPVTNTQGVSPINTKYAEHVVKNIYPEIKHDYFNESPNIYDK
                     KYISGITRGVAELKQEEFVNEKARRFSYMKT"
     gene            complement(275..>771)
                     /gene="nleB"
                     /locus_tag="N7C74_005119"
     CDS             complement(275..>771)
                     /gene="nleB"
                     /locus_tag="N7C74_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HGU2434768.1"
                     /translation="PEGKDSLIESYAEKREHTWFDFFRNLAMLKAGSLFTETGKTGCH
                     NISPCSGCIYLDADMIITDKLGVLYAPDGIAVHVDCNDEIKSLENGAIVVNRSNHPAL
                     LAGLDIMKSKVDAHPYYDGLGKGIKRHFNYSSLHDYNAFCDFIEFKHENIIPNTSMYT
                     CSSW"
BASE COUNT          242 a          162 c          116 g          251 t
ORIGIN      
        1 tagtcttcat ataagaaaac cgtctggctt tctcgttaac aaattcttcc tgttttagtt
       61 cagctactcc tctggttata ccggatatat acttcttatc atatatattg ggtgattcat
      121 taaagtaatc atgtttaatt tccgggtaaa tatttttcac cacatgttca gcatatttag
      181 tatttatagg ggacacgccc tgagtattag taacaggatt aatcatctga atctatacct
      241 aaagaacgat atttttcaac cacaaccgga agacttacca tgaactgcag gtatacatac
      301 tggtattcgg tataatattt tcatgcttaa attcaataaa atcacaaaaa gcattataat
      361 cgtgtaacga tgaatagtta aaatgccgct tgataccctt tcctagacca tcataatatg
      421 gatgagcgtc aactttactc ttcataatat cgaggcctgc aagtaatgct ggatgattac
      481 tacgattgac aactatcgca ccattttcaa gactttttat ctcatcatta caatctacat
      541 gcacagcgat accatcagga gcatacagga ctcctaattt atcggtaata atcatgtcgg
      601 catcaagata tatacatccg ctacagggcg atatgttatg gcatccagtt tttcccgtct
      661 ctgtaaacaa acttccagcc ttcaacatgg ccaaattcct gaaaaaatca aaccaagtat
      721 gttctctttt ttctgcatat gactcaatca aagagtcctt accttctggg a
//
LOCUS       DBFIFM010000504          767 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.516, whole genome shotgun sequence.
ACCESSION   DBFIFM010000504 DBFIFM010000000
VERSION     DBFIFM010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.516"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..>767
                     /locus_tag="N7C74_005120"
     CDS             <1..>767
                     /locus_tag="N7C74_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS domain-containing protein"
                     /protein_id="HGU2434769.1"
                     /translation="TDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYEEPLVESRY
                     LYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQP
                     GSFTPLIRVETATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEILA
                     DRVSEESRRWLASCGLTVAQMQSQMDPVYTPARKIHLYHCDHRGLPLALISKEGATEW
                     CAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLY"
BASE COUNT          175 a          219 c          252 g          121 t
ORIGIN      
        1 aaccgacctc atcccggaag gggttatccg cacggatgat gagcggactc accggtacca
       61 ttacgacagt cagcaccggc tggtgcacta cacgcggaca caatatgaag agccgctggt
      121 cgaaagccgc tatctttacg acccgctggg ccgcagggtg gcaaaacggg tgtggcgacg
      181 tgaacgggac ctgacgggct ggatgtcgct gtcacggaaa ccgcaagtga cctggtacgg
      241 ctgggacggc gaccggctga ccacaataca gaacgacaga acccgcatcc agacgattta
      301 tcagccgggg agcttcacgc cactcatcag ggttgaaacc gccaccggtg agctggcgaa
      361 aacgcagcgc cgcagcctgg cggatgcgct tcagcagtcc ggcggcgaag acggtggcag
      421 tgtggtgttc ccgccggtgc tggtgcagat gctcgaccgg ctggaaagtg aaatcctggc
      481 tgaccgggtg agtgaggaaa gccgccgctg gctggcatcg tgcggcctga ctgtggcgca
      541 gatgcaaagc cagatggacc cggtatacac gccggcgcga aaaatccacc tgtaccactg
      601 cgaccatcgc ggcctgccgc tggcccttat cagtaaggaa ggggcaacag aatggtgcgc
      661 agaatacgat gagtggggca acctgctgaa tgaagagaac ccgcatcagc tgcagcagct
      721 tatccgcctg ccggggcagc agtatgatga ggagtccggc ctgtatt
//
LOCUS       DBFIFM010000505          765 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.517, whole genome shotgun sequence.
ACCESSION   DBFIFM010000505 DBFIFM010000000
VERSION     DBFIFM010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.517"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..166)
                     /locus_tag="N7C74_005121"
     CDS             complement(<1..166)
                     /locus_tag="N7C74_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HGU2434770.1"
                     /translation="MKACLLLFFYFSFICQLHGADVKIKQNESMMGSTAMTYDLSEEK
                     LMKLKYKSQHG"
     gene            complement(178..>765)
                     /locus_tag="N7C74_005122"
     CDS             complement(178..>765)
                     /locus_tag="N7C74_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU2434771.1"
                     /translation="GRYITQDPIGLKGGWNFYQYPLNPVINVDPQGLVDINLYPESDL
                     IHSVADEINIPGVFTIGGHGTPTSIESATRSIMTAKDLAYLIKFDGNYKDGMTVWLFS
                     CNTGKGQNSFASQLAKELHTNVIGPDTLWTWWGRGTNGKLKMDTVLTAPTNLNSNKDL
                     MAITTKDLGNWITYGPSGHPISNMQGTPEKPSDIR"
BASE COUNT          217 a          169 c          123 g          256 t
ORIGIN      
        1 caccatgttg tgatttatac ttcagtttca tcaacttttc ttcacttaaa tcataggtca
       61 ttgctgtaga ccccatcata ctttcgtttt gttttatttt cacatcagca ccatgcaatt
      121 gacaaataaa agagaaataa aaaaatagta acaagcatgc tttcatacat ctacaaccta
      181 tcttatatca ctgggttttt ctggcgtacc ttgcatatta gaaatggggt gcccagatgg
      241 cccatatgtt atccaattac caaggtcttt tgttgttata gccattagat ccttatttga
      301 attaaggttc gttggtgctg ttagcactgt atccattttt aacttaccat tagttcctcg
      361 cccccaccac gtccatagcg tgtcaggtcc tattacattt gtatgtaact ctttagctaa
      421 ttggctagca aatgaatttt gtcctttacc tgtattacaa gaaaataacc aaactgtcat
      481 cccatcttta taattcccat caaatttaat tagatatgct agatctttag ctgtcatgat
      541 actgcgcgtt gcggattcaa tagatgtggg ggtaccatgc cccccgattg tgaaaacgcc
      601 tgggatatta atctcatcag ctacagaatg gataagatca ctttcggggt ataaatttat
      661 atcaaccaaa ccttgcggat ctacatttat gaccggattc aacggatact gataaaaatt
      721 ccatcccccc ttcagtccaa tcggatcctg agtgatatat cgccc
//
LOCUS       DBFIFM010000506          763 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.518, whole genome shotgun sequence.
ACCESSION   DBFIFM010000506 DBFIFM010000000
VERSION     DBFIFM010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.518"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..>763
                     /gene="espL4"
                     /locus_tag="N7C74_005123"
     CDS             <1..>763
                     /gene="espL4"
                     /locus_tag="N7C74_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HGU2434772.1"
                     /translation="LAINYGNADIVETIFNSLSEPGYEGLLSKKNLMHILEAKDKNGF
                     SGLFLAISRKDKNVVTSILNALPKLAATHHLDNEQVYKFLSAKNSTSSHVLYHVMANG
                     DADMLKIVLDALSLLIRTCHLTKEQVLDLLKAKDFYGCPGLYLAMQNGHSDIVKVILE
                     ALPSLAQEINISASDIVDLLTAKSLARDTGLFMAMQRGHMNVINTIFNALPTLFNTFK
                     FDKKNMKPLLLANNSNEYPGLFSAIQHKQQNVVEMV"
BASE COUNT          237 a          143 c          152 g          231 t
ORIGIN      
        1 atttggctat aaattatggt aatgcggata tcgttgagac tatttttaat tcattgtcag
       61 agccaggata tgaaggatta ctctcgaaaa aaaatctcat gcatattctg gaggcaaaag
      121 ataaaaatgg tttttctgga ttatttttag cgatatcacg taaggataaa aatgttgtaa
      181 cctcgattct gaacgcctta cctaaactgg ccgcaacaca tcatttagat aacgaacaag
      241 tgtataaatt cctgagtgcc aaaaatagta cgtccagcca tgttttatac catgttatgg
      301 cgaatggtga tgccgacatg ctgaaaattg ttttggacgc gttatctttg ttaattcgca
      361 catgtcattt gactaaagaa caggtactcg atctcctgaa ggcaaaggat ttttatggtt
      421 gcccaggact atacctggcg atgcaaaatg gacatagcga tatcgtgaaa gttattcttg
      481 aagcattgcc cagcctggcc caggaaatta acatttcagc ttccgatatt gtagatcttc
      541 tgaccgctaa aagtcttgcg cgcgacacgg gtttgtttat ggccatgcag cgcggacata
      601 tgaacgttat taatactatt tttaacgcat tacccactct gtttaatacg tttaaattcg
      661 ataaaaaaaa tatgaagccc ctcctcctgg caaataattc taatgaatac ccaggtttgt
      721 tttcagcgat acagcataaa cagcaaaacg ttgtagagat ggt
//
LOCUS       DBFIFM010000507          763 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.519, whole genome shotgun sequence.
ACCESSION   DBFIFM010000507 DBFIFM010000000
VERSION     DBFIFM010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.519"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(114..190)
                     /locus_tag="N7C74_005124"
     tRNA            complement(114..190)
                     /locus_tag="N7C74_005124"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(154..156),aa:Arg,seq:tct)
     gene            complement(204..280)
                     /locus_tag="N7C74_005125"
     tRNA            complement(204..280)
                     /locus_tag="N7C74_005125"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(244..246),aa:Arg,seq:tcg)
     gene            complement(288..363)
                     /locus_tag="N7C74_005126"
     tRNA            complement(288..363)
                     /locus_tag="N7C74_005126"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(328..330),aa:Ile,seq:cat)
BASE COUNT          208 a          194 c          175 g          186 t
ORIGIN      
        1 catttaaata atatacactt catatacagg tgttcctttt ggctgaagta atcagcacca
       61 gacccggcgc agatataaaa aaggcccgca aaagcgagcc tggtaaataa atatggcgcg
      121 ttgtactgga ttcgaaccag tgaccgattg ctcagaaggc aattgctctg tccggctgag
      181 ctaacaacgc ataatgcaga taatggaccg ccatcgagga ctcgaacccc gcgcagccag
      241 cttcgaaggc tggcgctcta tcccgatgag ctaatggcgg tatgtgatgg tggcccttgc
      301 tggatttgaa ccagcgacct ggcgattatg agtcgctcgc tctcaccact gagctaaagg
      361 gccgggcgca ggataataac ggtacgtaac taattctgca atatcatccg ttctgactga
      421 ctacattctg aacttccctg accgtctgct caaaacgccc gctctccagc tcaacgccaa
      481 ttgcacgacg ccctagcgcc attgctgctt tgacgctacg gacataaaaa agccagccac
      541 tgggggaggc tggcaaactc gtagagcaaa atgctgttac gcaaacttcg ttacagggtt
      601 atcctgcaat acttaaaata tacaatattt agaaaactaa tagtgctata tgcgattttt
      661 aagattttgt tattaattac ggtcgcacct tcctttctgt gtactttccg tatagctcac
      721 aggattcggg gtacaaaaaa acccgcgcat cggcgggttc gac
//
LOCUS       DBFIFM010000508          762 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.520, whole genome shotgun sequence.
ACCESSION   DBFIFM010000508 DBFIFM010000000
VERSION     DBFIFM010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.520"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            147..617
                     /gene="argR"
                     /locus_tag="N7C74_005127"
     CDS             147..617
                     /gene="argR"
                     /locus_tag="N7C74_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HGU2434773.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT          212 a          185 c          164 g          201 t
ORIGIN      
        1 gacgcaggca tatttctcaa taacgaaatt tgataaaatc ccgctctttc ataacattat
       61 ttcagccttc ttcagggctg actgtttgca taaaaattca tctgtatgca caataatgtt
      121 gtatcaacca ccatatcggg tgacttatgc gaagctcggc taagcaagaa gaactagtta
      181 aagcatttaa agcattactt aaagaagaga aatttagctc ccagggcgaa atcgtcgccg
      241 cgttgcagga gcaaggcttt gacaatatta atcagtctaa agtctcgcgg atgttgacca
      301 agtttggtgc tgtacgtaca cgcaatgcca aaatggaaat ggtttactgc ctgccagctg
      361 aactgggtgt accaaccacc tccagtccat tgaaaaatct ggtgctggat atcgactaca
      421 acgatgcagt tgtcgtgatt cataccagcc ctggcgcggc gcagttaatt gctcgcctgc
      481 tggactcact gggcaaagca gaaggtattc tgggcaccat cgctggcgat gacaccatct
      541 ttaccacccc tgctaacggt ttcaccgtca aagacctgta tgaagcgatt ttagagctgt
      601 tcgaccagga gctttaatct ctgccccgtc gcttctggcg gcggggaaaa tgttgcttat
      661 ccctctcaac cccctgcttt cccctgcgat taatttaacg aatagtgcgt tttactgcga
      721 catgtcattc acacaatgaa tacataaggt aaaaaaagca ca
//
LOCUS       DBFIFM010000509          756 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.521, whole genome shotgun sequence.
ACCESSION   DBFIFM010000509 DBFIFM010000000
VERSION     DBFIFM010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.521"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            66..>756
                     /locus_tag="N7C74_005128"
     CDS             66..>756
                     /locus_tag="N7C74_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434774.1"
                     /translation="MSIDFTPGMINTYHGDIYNRTTDTDNVKTPDTPMWPCDNREEQQ
                     PINSTFSGEGYNPEQYDLARQHLPQIYACRTNTKYTDADYSKVVAQLVSLITNIESIS
                     STPLTRQAQEILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPVRFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNM
                     TGQNLSGFVLTL"
BASE COUNT          282 a          154 c          127 g          193 t
ORIGIN      
        1 cattcctgct ttaattatcc aatttaaaaa tcttagcata aaacagataa tgaataagga
       61 atgttatgtc aattgacttt accccaggta tgataaatac atatcacggc gatatttata
      121 accgcacaac agataccgac aatgttaaaa caccagacac accgatgtgg ccttgtgata
      181 atcgggaaga acaacaaccc attaattcca cattctctgg agaaggatac aaccctgaac
      241 aatatgattt agcgcggcaa cacctcccac aaatttatgc gtgccgcacg aacaccaaat
      301 atacagatgc agactactca aaagttgtgg ctcaacttgt aagtcttatt actaacattg
      361 aatcgataag ttcgacacca cttacgcggc aggcacagga aatacttaat caaattaata
      421 acattagata cgaaaagaac aaaagtgctg aatgtcgaat cattgttgtc gctaatccta
      481 aacccgacaa ggcaatcata actaaaatta gcgtagaaga gggaatacca gtaagattca
      541 gtgtccaaac catgttttca gatactaatt ttattgctga acaacgagct gacttaccga
      601 ctaatataaa agatattcaa agcctgtatc aaaaaatgac gaagttatat attgagcaca
      661 gtgaaaataa aaacaggatg aaagtctttg ccggaaccaa ctttattgat ttcaatatga
      721 caggacagaa cctgtccggg ttcgttctaa ccttgt
//
LOCUS       DBFIFM010000510          755 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.522, whole genome shotgun sequence.
ACCESSION   DBFIFM010000510 DBFIFM010000000
VERSION     DBFIFM010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.522"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..749
                     /locus_tag="N7C74_005129"
     CDS             <1..749
                     /locus_tag="N7C74_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU2434775.1"
                     /translation="SNHLPVDELHNTSQFIIENILQTVHNPQRSVRLTKQDQGYKNHY
                     LSDEMLAGKKELYEFTPESIYRAMTIFDGLQNKSDIQTLKTECYCLLAECHMSLALHG
                     KSELELAAQKALELLDYVSDITTVDGKILAIMGLITGLSGQAKVSHILFEQAKIHSTD
                     IASLYYYRALVNFHNEKIEEARICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKKNMK
                     LYYKETESGSHRVIIDNILKLKQLTRICMR"
BASE COUNT          285 a          130 c          132 g          208 t
ORIGIN      
        1 ccagtaatca tctacctgtt gatgaactac ataatacatc acaatttatt atagaaaata
       61 tccttcaaac agtacataat ccacaacgat ctgtaagatt aactaagcag gaccaaggat
      121 ataagaatca ttatttatca gatgaaatgt tagccggaaa gaaagaactt tacgagttca
      181 cccctgaaag catttacagg gccatgacta tatttgatgg attacaaaat aaaagtgata
      241 tacagacgct aaaaacagaa tgttattgcc ttctagcgga atgccatatg tctttggcac
      301 ttcatggaaa aagtgaactt gaacttgctg ctcaaaaagc attagagctt ttagattatg
      361 tatcagacat aaccactgtc gatggaaaaa ttttagctat tatgggactg ataactggtc
      421 tgtctggaca agcaaaagta tctcatatct tatttgaaca ggctaagata cactcaactg
      481 atatagcctc tctctactac tatagggcac ttgtcaactt tcataatgaa aaaattgaag
      541 aggcaaggat ttgtatagac aaatcactac aactcgaacc cagaagacga aaagcagttg
      601 tgataaaaga atgtgtagat atgtatgtgc ctaacccgct caaaaaaaac atgaaactct
      661 actataaaga aactgagagt ggaagccatc gagttataat tgacaacatt ttgaaattaa
      721 agcagctgac gagaatttgt atgcgataaa ttttt
//
LOCUS       DBFIFM010000511          754 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.523, whole genome shotgun sequence.
ACCESSION   DBFIFM010000511 DBFIFM010000000
VERSION     DBFIFM010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.523"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            147..716
                     /locus_tag="N7C74_005130"
     CDS             147..716
                     /locus_tag="N7C74_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HGU2434776.1"
                     /translation="MNMMAVPFHGNSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKQRNMVSMPLRKLAGWLQTINPNKVKPEIRDKVIRYQEEC
                     DDVLYEYWTKGFVVNPRKMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLVNEANMLIDFVLADTGKGKITKAD"
BASE COUNT          214 a          159 c          194 g          187 t
ORIGIN      
        1 gcctttgtca tttctgcgca tctcacgcgc atctcacaac acagaacctt tcaggatgac
       61 ccttgaggat accggtttgg ctgtcggtgc ctttctgtgg gctggattcc tgtgagacaa
      121 ggttcatcac taaaaggaaa taaccgatga atatgatggc cgtgccgttt cacggcaact
      181 ctctttatgt agttaaccat aatggcgaac catacgttcc catgaaacct gtcgttgcgg
      241 ggatggggct ggcctggcaa tcacagttgg ctaagttaag acagcgtttt gcgtcaacta
      301 taacggaaat cgttatggtt gctgaggatg ggaaacaacg caatatggtg tccatgccac
      361 ttcgaaaact tgccggctgg ctacaaacca ttaatcccaa caaagtaaaa cccgaaatcc
      421 gcgataaggt catccggtat caggaagagt gcgacgatgt tctttacgag tactggacga
      481 agggttttgt cgttaatccc cgtaaaatga gcgtgatgga agaactcaac caggcttgtg
      541 ctgacatgaa acgggataaa aacattgcca gtgtgtttgc taccgggctg aatgagtgga
      601 aacaggttaa agccgcgcat gtatcaaaaa tccgtacgct ggtaaatgaa gcgaatatgc
      661 tgattgattt tgtcctggct gatacaggca aagggaaaat aacaaaggcg gattgatggg
      721 gtggctaatg atatcagata aactcataac gctg
//
LOCUS       DBFIFM010000512          747 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.524, whole genome shotgun sequence.
ACCESSION   DBFIFM010000512 DBFIFM010000000
VERSION     DBFIFM010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.524"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..428
                     /locus_tag="N7C74_005131"
     CDS             <1..428
                     /locus_tag="N7C74_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HGU2434777.1"
                     /translation="KSTVSVFYLQPEEVNTLSHQAKRGDGEAGFRLFLYYKLSNYDEE
                     KSSQWLKIAANNGHATAQYRLYVELQEQGETKEANEWLQKIKLAASNNDSSAIWFLVN
                     CYKNQGNKKEALQWAYKLKASKQPLDADRLIEELTKEQK"
BASE COUNT          279 a          129 c          156 g          183 t
ORIGIN      
        1 caaaatcaac agtaagcgtc ttttatttgc agccagaaga agtaaatact ttatcacacc
       61 aggcaaaacg aggcgatggc gaggcgggtt ttaggttgtt cttgtactac aagttgtcca
      121 attatgatga agaaaaaagt agtcagtggt taaaaatagc ggctaataat ggtcatgcga
      181 cagctcagta tcggttatat gtagagttac aagaacaagg tgaaaccaag gaagctaatg
      241 aatggttaca aaaaattaaa ttggctgcca gtaataatga cagttcagca atatggttct
      301 tggtgaattg ttataaaaac cagggaaata aaaaagaagc attgcaatgg gcatataaac
      361 tcaaagcgag taaacagccg cttgatgcag atcgactaat tgaagaatta acgaaagaac
      421 aaaagtagct ttaaacaggt aacagaaaaa tggagagtag aaaaacttta ccagtcgcta
      481 caacacctac acacaaaatt acgtttacga cgactgcaac aagctcatca aagcgcaagt
      541 tgaaaccaaa actggcatct tcgtcgccgg ttatcattac gatgtacttg gcagatgcat
      601 caaaagtcgc aagagaaaag atgaaaatat agctttttgt atcattaagt atgtggccag
      661 cacatgactc ggcatagatc ttatgactaa gattctacac cccaaaccta agtatgggcc
      721 aaagaagccg tttggttcat tttttat
//
LOCUS       DBFIFM010000513          734 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.525, whole genome shotgun sequence.
ACCESSION   DBFIFM010000513 DBFIFM010000000
VERSION     DBFIFM010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.525"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            177..>734
                     /gene="lpfA2"
                     /locus_tag="N7C74_005132"
     CDS             177..>734
                     /gene="lpfA2"
                     /locus_tag="N7C74_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA2"
                     /protein_id="HGU2434778.1"
                     /translation="MKPNMIVGALALTSVFMAGHLQAADGTVHFRGEIIDSTCEVTPE
                     TKDQVVDLGKVNRTAFSGVDDVAAPTAFSIDLTQCPETFKSAAIRFDGNEDAHGNGNL
                     AIGTPLDNSNDAAAGISPSDNSGDYTGAGAVSAAKGVAIRLYNRADNTQVKLYENSAS
                     TPISNGNASMKFMARYIATETTIDPG"
BASE COUNT          215 a          141 c          159 g          219 t
ORIGIN      
        1 aatttagttt tgcttaacac ctaaattaat ttatctaaat gtataaaata aaaaccactt
       61 gcgccaaagt atattggtag tactatttgg tttctgatca aataccgatc aggtttatcc
      121 aaatttaata aatggataac acacaggaat aatatcaaat taatggagtt atatttatga
      181 aaccaaatat gattgtagga gcattagcgt taacttctgt gtttatggca ggtcacctac
      241 aggcggctga tggaacagtc catttccgtg gtgaaattat tgacagtact tgcgaagtca
      301 ctcctgaaac taaagatcag gtcgttgatt taggcaaagt aaaccgtaca gcctttagtg
      361 gcgtcgatga tgtggctgcc ccgacggctt tttctatcga tctgactcaa tgcccggaaa
      421 cctttaagtc cgccgcaatt cgtttcgatg gtaatgaaga tgctcatggt aatggcaacc
      481 tggcaattgg taccccgctg gataactcta acgatgctgc cgctggtatt agcccgagtg
      541 ataacagtgg ggattatact ggtgcgggtg ccgttagtgc agcgaaaggc gtagctattc
      601 gtttatataa ccgtgcagat aacactcagg tcaagttata tgaaaattct gcatcaactc
      661 cgatttctaa tggtaatgca tccatgaagt tcatggctcg ttatattgct acggaaacga
      721 ctattgaccc tggt
//
LOCUS       DBFIFM010000514          732 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.526, whole genome shotgun sequence.
ACCESSION   DBFIFM010000514 DBFIFM010000000
VERSION     DBFIFM010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.526"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            506..694
                     /locus_tag="N7C74_005133"
                     /pseudo
     CDS             506..694
                     /locus_tag="N7C74_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          216 a          142 c          155 g          219 t
ORIGIN      
        1 ctactgatgt acaaaacaca acaatagctt catgatatat aaatacgtga tgacgagtgg
       61 tactccgcat ggttatttaa cttctaagca ccactaatga tcctgagcaa cttcgtattt
      121 gggccattac catggagtag aaagctgtgt ctgagaatgc agaatttgag aaaagaattc
      181 gctttcttgg agaaattctg aaaatggcac gctaacaacg ttttgttaaa aaatgtgaat
      241 tacttgccgg ggctgcattt tctattcgag gagaatgttg acgtagatat tacagaaatc
      301 tcagcgcatc aggataaact gctaccttag gtcaaccacc aaaagaaaat acaatacgtc
      361 cagtttgtat gcctttaata acacatcttc taaatgttga agaattatcc cgcctgctga
      421 agaacgttgc tctgagtgta ataaaccttt ttattcttga tgatgtcagc gagaagatgg
      481 aatatatccc cgactgggtc gtggttaccc ggttaaacat ctggtggtgc taataaatct
      541 ggcaagtcac attctgttga atgctgtgac agtgccttac cgagagagcg aaattgtggt
      601 gttctagtgc atagtcgcca ccattccgga taattctatt acgttgtttt acaagttctt
      661 ttacagcgca gatctgtcgc tgacgttgaa tcaatagcct ggatttcaga acacaatgcc
      721 cgtttgctgg tt
//
LOCUS       DBFIFM010000515          730 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.527, whole genome shotgun sequence.
ACCESSION   DBFIFM010000515 DBFIFM010000000
VERSION     DBFIFM010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.527"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(94..636)
                     /gene="fimA"
                     /locus_tag="N7C74_005134"
     CDS             complement(94..636)
                     /gene="fimA"
                     /locus_tag="N7C74_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGU2434779.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT          216 a          157 c          145 g          212 t
ORIGIN      
        1 atttcgattt acattccacc ataccgcttc agacaaccgg tacattactt tgttataaga
       61 cgtgatatat aattcaaaac aacgtggttt tgattattcg tatctcataa taaaagtcgc
      121 gtcagcattt gcttgccctg ctgatgcact tgtaccggtg cctttataac gtgcagaaaa
      181 atgaagaacg ttggtcccgg ggatcaagtt ctggttggtt gagaagctat tcccatcagg
      241 tttgagaatt gcggatgtat tatcaagtat ttcaataccg acacccgttg ctgttgttgt
      301 attggtactg gatgcaatgg ccaataaatt atcattgatt gcatcagcct gaccagaaaa
      361 tgcaacggca gcattagcgg caacaacagg atcgcagtcg ttcaactgaa tggtgaatgg
      421 aattaatgca gaggtattac caacagcatt gaaaatatcg gtacgatatt gaccgagtgt
      481 gacaacctgg tctgccgagt cagtattcac tgaacaggca gcattcacca gttcaccttc
      541 aaagtgaatt gtaccgccat ctacaacggc agcataacta cccgtagcgg cgaatagtgc
      601 ggcagccagc gcagacgaaa taaatcttaa tttcatatat attccttcaa tctcatttat
      661 cgactccaca tccgtatata accgattact ttatttaaga cactgatagt agtaaattcc
      721 tttttatcct
//
LOCUS       DBFIFM010000516          729 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.528, whole genome shotgun sequence.
ACCESSION   DBFIFM010000516 DBFIFM010000000
VERSION     DBFIFM010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.528"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            9..>729
                     /locus_tag="N7C74_005135"
     CDS             9..>729
                     /locus_tag="N7C74_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2434780.1"
                     /translation="MIEEGLLLPMNVLLQGQKLTLLDPEMWFLRGNENKDVTLVITIG
                     NNHQKLMVVEDILLLIDRSQIEVTAGKVIYHPLRIDILSKLLAFIDESTGSVEREHHE
                     LFADALPFASEVVLFSKVASEAWFLATYLSSDNINEILFQHLRKTECYKLVRYLLSQS
                     LIQTSLYDLGELYGVSYSHFRRLCSYALGGKVKTELCGWRVARAVLEIIEGNSDMTTI
                     AHKYGYSSSSHFSAEVKSRLGK"
BASE COUNT          227 a          122 c          161 g          219 t
ORIGIN      
        1 gatgaaacat gattgaagaa gggctgttac ttccaatgaa tgttttgctt caaggacaga
       61 agttaaccct actggaccct gaaatgtggt tcttacgggg aaatgaaaac aaggatgtaa
      121 cccttgttat tacgattggt aataaccatc aaaaattaat ggtagtggaa gatatattac
      181 tgctcattga ccgaagccaa attgaggtaa ctgcagggaa agtaatttat catccacttc
      241 gcattgatat cctgagtaaa ttattagctt ttatagatga atcaacgggg agtgtggaaa
      301 gggaacacca tgaattgttt gctgatgcct tgccctttgc atcagaagtt gtgctattca
      361 gtaaagttgc aagtgaagcc tggtttttag ccacttatct gagcagcgat aatattaatg
      421 aaatcctgtt tcagcatcta agaaaaacag aatgctataa actggttcgt tatttattat
      481 cgcaatcgct catacagact tcactctatg atcttggtga gctttacggc gtatcgtatt
      541 cacattttcg ccgtttatgt agttatgcat tgggtggtaa agttaaaacg gaattatgtg
      601 gctggagagt tgccagagcc gtgttggaaa tcattgaagg aaacagtgat atgacaacta
      661 ttgcgcataa atatggttac tcgtcctcat cacacttttc agccgaagta aaaagccggt
      721 taggaaaaa
//
LOCUS       DBFIFM010000517          727 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.529, whole genome shotgun sequence.
ACCESSION   DBFIFM010000517 DBFIFM010000000
VERSION     DBFIFM010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.529"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(8..>727)
                     /locus_tag="N7C74_005136"
     CDS             complement(8..>727)
                     /locus_tag="N7C74_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU2434781.1"
                     /translation="LSERLKDQAVFYAESDVQRADWQKGLQEGFSNWVDNASDYASQA
                     AQLATEGISGMVNNITEMLNGNKVEWRSWASSVLQEISKVLMNAAIVNGIKTAANGMS
                     GAGGFLGSIGDWLGGAVANAKGGVYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGP
                     EAIMPLTRAADGSLGVRAVGSMNGSAGLVYSPVYHIAIQNDGANGQIGPEAAGSLVQL
                     IDQRVQAVMLSMRRDGGMLSG"
BASE COUNT          139 a          253 c          166 g          169 t
ORIGIN      
        1 ttttatctca gccactcagc attcctccgt cacgtcgcat ggacagcatc accgcctgca
       61 cccgctggtc aatcagctgc acaagactgc ctgccgcctc cggccctatc tgtccgttag
      121 ccccgtcatt ctgaatggca atgtggtaga ccggggaata caccagacca gcactgccgt
      181 tcatactgcc cacggcgcgt acgcccagcg agccatccgc cgcccgggtc aggggcataa
      241 tagcttcagg tccggcttcc cccatcagcc cggccccttt tgcaaacgca aagtacgtgg
      301 gcgtgtccac aatgctgttg ctgtacgcgc tcaggtttgc cgaggtatac acgccgcctt
      361 ttgcattggc caccgctccg cccagccagt caccaatgct gccgagaaat cctcccgcac
      421 cggacatacc gtttgccgcc gtcttaattc cgttgacaat cgcggcattc ataagaactt
      481 ttgatatttc ctgcagtacg gatgaggccc agctgcgcca ttccacttta tttccgttca
      541 gcatctccgt gatgttattc accatccctg agataccctc cgtcgccagc tgtgctgcct
      601 gtgaggcgta atcggacgca ttatccaccc agttactgaa tccctcctgc agccctttct
      661 gccagtccgc acgctgcaca tccgattcgg cataaaagac tgcctggtcc ttaaggcgtt
      721 cgctcag
//
LOCUS       DBFIFM010000518          720 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.530, whole genome shotgun sequence.
ACCESSION   DBFIFM010000518 DBFIFM010000000
VERSION     DBFIFM010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.530"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(59..>720)
                     /locus_tag="N7C74_005137"
     CDS             complement(59..>720)
                     /locus_tag="N7C74_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU2434782.1"
                     /translation="FRYAIATGRAEYNPAADLSSALEVHQSNHFPFLKADEIPDFLRA
                     LEGYSGSKLVQIATKLLMITGVRTIELRAALWQEFDLDNAIWEIPAERMKMRRPHLVP
                     LSSQAVDLLNELKIMTGNYRYVFPGRNDPNRPMSEASINQAIKRIGYGGKVTGHGFRH
                     TLSTILHEQGFESAWIEIQLAHVDKNSIRGTYNHAQYFSGRKSMMDWYSNLIFERLKR
                     S"
BASE COUNT          201 a          174 c          134 g          211 t
ORIGIN      
        1 atacctccca ttgttcatca tcccatgtca atatttccga aagtggttga gacacaaatt
       61 aactcctttt tagtctttca aatatcaaat tactgtacca gtccatcata gacttccttc
      121 cactaaaata ttgagcatgg ttataagtcc ccctaataga atttttatct acatgagcca
      181 actggatttc aatccaagca ctctcaaaac cttgctcatg caggattgta gaaagggtat
      241 gacgaaaacc atgtccagtg acttttcctc catacccaat acgcttaatg gcttgattta
      301 tactcgcttc gctcattggc ctattcggat cgttccgccc tggaaaaaca taacgatagt
      361 tccctgtcat gatcttgagt tcattgagta aatctaccgc ttgagatgat aagggcacaa
      421 gatgtggcct acgcattttc atcctttcag caggaatttc ccaaatagcg ttatccagat
      481 caaattcttg ccataatgcc gcgcgtaatt cgatggttct cacacccgta atcatcagta
      541 atttcgtggc tatctggaca agcttactcc cggagtaacc ctctaaggca cgtagaaaat
      601 caggtatctc atcagctttt aggaatggga aatgattgga ttggtgtact tcgagagcgc
      661 tggagagatc agccgcagga ttgtactccg ccctacccgt tgcaattgcg tagcgaaaca
//
LOCUS       DBFIFM010000519          718 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.531, whole genome shotgun sequence.
ACCESSION   DBFIFM010000519 DBFIFM010000000
VERSION     DBFIFM010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.531"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(122..461)
                     /locus_tag="N7C74_005138"
                     /pseudo
     CDS             complement(122..461)
                     /locus_tag="N7C74_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          187 a          181 c          159 g          191 t
ORIGIN      
        1 aaggctgggc catactcctc cggcagaagc agaaaaagct tattatgctt ccatcggaaa
       61 cgatgatctg gcagcctgag ttcacagata aaacactctc caggaaaccc ggggcggttc
      121 aaacctgatt ctggaaaagg actttgtgga ttgttgctat tgccagtgag attgaatgaa
      181 gtcgttttaa ctcgctttga atacgtcttg cccccagatt tcgctgagaa cgtaactcca
      241 gaatcagagc gacttcgcaa gtgccggttt tcgttgatgg tgaacgcttg gggcgacggc
      301 tgtggctttc cagaccagcg atgccctgag ccagatatct gcgccaccat tttacgcaat
      361 gtgggccgag aaatgccaca gcgacggcat acaaatcccg catcaccgga cgtttcatag
      421 agttttaccc actgtagccg ctgctgaatt tccctgttca taatttggca ttagttcagc
      481 tcaatcccga cttaaaaagt ccctacagga tgttccggac ctgaggcaca gttcatatcc
      541 ttatccataa aaaatttagc tatgtctata gcattaatct tgacaagacg acgttagggc
      601 ctggcgatga atcatttccc ctgccagata tcctttgggg tgcaccagaa ctaccgtttt
      661 atatctatat aactgaaacg tccctcgcca tccttcatat tttgatgtag taacacca
//
LOCUS       DBFIFM010000520          718 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.532, whole genome shotgun sequence.
ACCESSION   DBFIFM010000520 DBFIFM010000000
VERSION     DBFIFM010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.532"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            183..446
                     /gene="ykgM"
                     /locus_tag="N7C74_005139"
     CDS             183..446
                     /gene="ykgM"
                     /locus_tag="N7C74_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HGU2434783.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            446..586
                     /gene="ykgO"
                     /locus_tag="N7C74_005140"
     CDS             446..586
                     /gene="ykgO"
                     /locus_tag="N7C74_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HGU2434784.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(656..>718)
                     /locus_tag="N7C74_005141"
     CDS             complement(656..>718)
                     /locus_tag="N7C74_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434785.1"
                     /translation="VRQLTLNEQEDTSPGKLMLV"
BASE COUNT          208 a          144 c          164 g          202 t
ORIGIN      
        1 tgtacgctta ccaatcactc atccaggcgt ttatgggagc gtcgccaatg gctgttaaca
       61 atgccagact tccccgttga ggaaattcca cataccacta atggtcacag ggattgggtg
      121 ttgaaatgat ccggatgagc atgtatcttt gtggttatgt tataacataa caggtaaaaa
      181 tgatgaagcc caatatccat cctgagtatc gtactgtggt gttccacgac accagtgttg
      241 atgagtactt taaaatcggc tcgactatca aaacagaccg tgagattgag ctggatggcg
      301 taacgtatcc gtacgtaaca attgatgtct cttcgaaatc gcacccgttc tatacaggga
      361 agctgagaac agtggcatca gaaggaaatg ttgcacgatt cacccaacgt tttggtcgtt
      421 ttgttagcac gaaaaagggg gcgtgatgaa agtccttaac tctctgcgta ccgcaaaaga
      481 acgccatcca gactgtcaga ttgtgaagcg aaaaggacgg ctatatgtga tttgtaaatc
      541 taatccacgc tttaaggccg ttcagggtcg taagaaaaaa cgttgattta aaattcgacg
      601 aattaacgat atttgtctga ttaataatca gaccggatta atgttggtgt atttattaca
      661 ccagcattaa ttttcctggg gatgtatctt cctgttcatt taaggtcaac tgcctgac
//
LOCUS       DBFIFM010000521          716 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.533, whole genome shotgun sequence.
ACCESSION   DBFIFM010000521 DBFIFM010000000
VERSION     DBFIFM010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.533"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..456)
                     /locus_tag="N7C74_005142"
     CDS             complement(<1..456)
                     /locus_tag="N7C74_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HGU2434786.1"
                     /translation="MPLTSDIRSHSFNLGVEVVRARIVANGRGDITVGGETVSIVYDS
                     TNGRFSSSGGNGGLLSELLLLGFNSGPRALGERMLSMLSDSGEAQSQESIQNKISQCK
                     FSVCPERLQCPLEAIQCPITLEQPEKGIFVKNSDGSDVCTLFDAAAFSRL"
     gene            complement(570..>716)
                     /locus_tag="N7C74_005143"
     CDS             complement(570..>716)
                     /locus_tag="N7C74_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU2434787.1"
                     /translation="ENGIIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENI
                     GGMK"
BASE COUNT          216 a          196 c          122 g          182 t
ORIGIN      
        1 caaacgagaa aatgcagcgg catcaaataa agtacataca tctgaaccat ctgaattctt
       61 cacaaaaata cctttttcag gctgctccag tgtaattgga cactgaatag cctcaagcgg
      121 gcactgaagt ctctctggac aaacagaaaa cttacattga gatattttgt tctgaatact
      181 ctcttgcgat tgtgcttcac ctgagtccga aagcatactt agcattctct caccaagggc
      241 tcgaggacca ctattaaatc ccaaaagcaa taactcagaa agcaatccgc cattaccgcc
      301 actggatgaa aagcgcccat tagtagaatc atacacaata ctgacagttt caccaccgac
      361 tgtaatatct ccgcgcccat tggctacaat tcgggcacga acaacctcca ccccaagatt
      421 aaatgaatgt gatctaatat ctgaggttaa tggcataaca aagctcccta ttttaatttg
      481 aactccagac ttaaatagct gtaacaaaca tcccccatac gtgacaacaa aaaaccggag
      541 ccggactccg gtttttgtga agctgtcggc tatttcatcc cgccaatatt ttcccacgtc
      601 ccgtcagcac gcaggatttg cagcggtctt accacacact gtatctgctt tttatccgca
      661 tccagtatca ccacctgcgt gattaccctg tcctgctccg ggataatacc attctc
//
LOCUS       DBFIFM010000522          712 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.534, whole genome shotgun sequence.
ACCESSION   DBFIFM010000522 DBFIFM010000000
VERSION     DBFIFM010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.534"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..158)
                     /gene="ybfC"
                     /locus_tag="N7C74_005144"
     CDS             complement(<1..158)
                     /gene="ybfC"
                     /locus_tag="N7C74_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HGU2434788.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMW"
     gene            complement(155..>712)
                     /locus_tag="N7C74_005145"
     CDS             complement(155..>712)
                     /locus_tag="N7C74_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU2434789.1"
                     /translation="GRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGG
                     YGARPNKPPTPDPSKFPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKW
                     VKPNLTCTPQDDPQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGP
                     ATAGIDDLGEILAKMKQRTSRGIRK"
BASE COUNT          193 a          153 c          136 g          230 t
ORIGIN      
        1 ctccacatgg ctttattatt taagacagga aacgcggaaa aaatagtacc cttagcgaaa
       61 atagtaaaac aatcatgatc tttagcttgt atttcgcaat cagctataac accaaaacta
      121 acaaatatca tcagcaaaaa aaacaaaact cttttcattt tcttattcct ctcgatgttc
      181 tctgtttcat tttagctaaa atttcaccca aatcatctat ccctgctgtt gctggcccat
      241 cgggtttatt aatgtcaggg aataaatagg gttctgcaca agtatatcct ggtggtaatt
      301 gagttgttgg ccaatttgtc tgtggcaggt aatcacttgc tgtctttgta tccatccctg
      361 gatactgggg atcatcctgt ggcgtacaag tcagatttgg ttttacccac ttcctgcaat
      421 acagtgtgta gtcgtaaggg cttaatgctc tgaagaatgt tttgaaaaca ttaggcgcac
      481 tactggcctg gtcaatagga tatggcagtc ttaattgttt cgctatgtct ggaaatttac
      541 ttggatcggg cgtaggcggt ttgttgggtc tcgctccata tccgccatta tttaagtgtc
      601 catattttga tgccagtccc attgaatcta tatactgaac cggattcaac ggatactgat
      661 aaaaattcca tccccccttc agtccaatcg gatcctgagt gatatacctc cc
//
LOCUS       DBFIFM010000523          709 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.535, whole genome shotgun sequence.
ACCESSION   DBFIFM010000523 DBFIFM010000000
VERSION     DBFIFM010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.535"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..>709
                     /locus_tag="N7C74_005146"
     CDS             <1..>709
                     /locus_tag="N7C74_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RTX toxin hemolysin A"
                     /protein_id="HGU2434790.1"
                     /translation="KLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTE
                     KIQYRDFEFRTGGIPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGN
                     YGNDRLYGDDGDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYN
                     ILSGGTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVYGQNYGHHTIADEGGKGDR
                     LHLSDISFDDIAFKRVGN"
BASE COUNT          227 a           79 c          199 g          204 t
ORIGIN      
        1 taaactaaca attgatgcaa caggagcatc aaaacctggt gagtatatag tttcaaaaaa
       61 tatgtatggt gacgtgaagg tattgcagga agtcgttaag gaacaggagg tgtcagtagg
      121 gaagcgaaca gagaaaatac aatatcgtga ttttgaattc agaaccggtg gaattcctta
      181 tgatgtaata gataatcttc attctgttga agagctcatt ggcggaaaac atgatgatga
      241 attcaaaggc ggtaagttta atgatatatt ccatggcgca gatgggaacg attatatcga
      301 aggtaattat ggtaatgatc gactatacgg cgatgatggg gatgattata tatccggagg
      361 acagggagac gaccagttat ttggtggtag tggaaacgat aaattgagtg gaggggatgg
      421 taataattat ctgacaggag gaagcggtaa tgatgagctt caggcacacg gagcttataa
      481 tattctgtca ggtggtactg gtgatgataa actttatggt ggtggtggta ttgatcttct
      541 ggatggaggg gaaggtaatg actatctgaa tggtggtttt ggtaatgata tttatgttta
      601 tgggcaaaac tatggtcatc atacaattgc agatgaagga ggtaaaggag atcgtttgca
      661 cttatctgat attagctttg atgatatcgc atttaagaga gttggaaat
//
LOCUS       DBFIFM010000524          699 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.536, whole genome shotgun sequence.
ACCESSION   DBFIFM010000524 DBFIFM010000000
VERSION     DBFIFM010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.536"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            89..299
                     /locus_tag="N7C74_005147"
                     /pseudo
     CDS             89..299
                     /locus_tag="N7C74_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140296.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            257..586
                     /locus_tag="N7C74_005148"
                     /pseudo
     CDS             257..586
                     /locus_tag="N7C74_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          191 a          156 c          165 g          187 t
ORIGIN      
        1 atattaataa aaataagaac gaagattaag ttatccagag gttactgttg cgtctgtggt
       61 cgtaacaaca tccgacgtgg tgatgtgtca aatgagacat tctgatgatc agagggttta
      121 tcgcgtatac ggatcgtggg tggcagaaaa ctttcagaat caggtttcca ttttaaacca
      181 gaaattgagt gagtttgccc catccataac cccacgcggt gagatcggat gtcattaaac
      241 aagccaggtc aacaggtcag tatgggaagg cgaacaactc gcaggaatgg gtgcgctaaa
      301 gttgctggat gataaacatg gcgaactgaa atcaatgcgt accgcgccaa attatttacg
      361 tcggggtgtc gccagtctga ttttacgcca cattttgcag gtcgcccatg acagatgcct
      421 tcatcgcctg agcttagaaa cgggtacaca ggctggattt acggcctgcc atcaacttta
      481 tttgaagcat ggtttcgttg attgcgaacc gtttgctgat tatcaatttg atccacacag
      541 tcgatttttg tcattgacgc tatgcgaaga taatgaattg ccatgagcca gacgcagcgc
      601 attcttgcat tcgacgtgct gcgcctttat ttatcaccag caggaaacgc cttgtccata
      661 gacgctcctt ccacatgcgt cacaagaaac ctctattcc
//
LOCUS       DBFIFM010000525          697 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.537, whole genome shotgun sequence.
ACCESSION   DBFIFM010000525 DBFIFM010000000
VERSION     DBFIFM010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.537"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..>697)
                     /locus_tag="N7C74_005149"
     CDS             complement(<1..>697)
                     /locus_tag="N7C74_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030598 - rRNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome-inactivating family protein"
                     /protein_id="HGU2434791.1"
                     /translation="EFTIDFSTQQSYVSSLNSIRTEISTPLEHISQGTTSVSVINHTP
                     PGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAGFVNTATNTFYRFSDFTHISVPGV
                     TTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSSYLALMEFSGNTMTRDASRAVLRF
                     VTVTAEALRFRQIQREFRQALSETAPVYTMTPGDVDLTLNWGRISNVLPEYRGEDGVR
                     VGRISFNNISAILG"
BASE COUNT          192 a          171 c          153 g          181 t
ORIGIN      
        1 cccagtatcg ccgatatatt attaaaggat attctcccca ctctgacacc atcctctccc
       61 cgatactccg gaagcacatt gctgattcgc ccccagttca gagtgaggtc cacgtctccc
      121 ggcgtcatcg tatacacagg agcagtttca gacagtgcct gacgaaattc tctctgtatc
      181 tgcctgaagc gtaaggcttc tgctgtgaca gtgacaaaac gcagaactgc tctggatgca
      241 tctctggtca ttgtattacc actgaactcc attaacgcca gatatgatga aaccagtgag
      301 tgacgactga tttgcattcc ggaacgttcc agcgctgcga cacgttgcag agtggtataa
      361 ctgctgtccg ttgtcatgga aaccgttgtc acaccgggca ctgatatatg tgtaaaatct
      421 gaaaaacggt agaaagtatt tgttgccgta ttaacgaacc cggccacata taaattattt
      481 tgctcaataa tcagacgaag atggtcaaaa cgcgcctgat agacatcaag ccctcgtata
      541 tccacagcaa aataactgcc cggtggggtg tggttaataa cagacaccga tgtggtcccc
      601 tgagatatat gttcaagagg ggtcgatatc tctgttcgta tactatttaa cgaagagaca
      661 taactttgtt gggtcgaaaa gtctatcgta aactccc
//
LOCUS       DBFIFM010000526          686 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.538, whole genome shotgun sequence.
ACCESSION   DBFIFM010000526 DBFIFM010000000
VERSION     DBFIFM010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.538"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..79)
                     /locus_tag="N7C74_005150"
     CDS             complement(<1..79)
                     /locus_tag="N7C74_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGU2434792.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTS"
     gene            complement(91..489)
                     /locus_tag="N7C74_005151"
     CDS             complement(91..489)
                     /locus_tag="N7C74_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HGU2434793.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVKLHTDALHVTRDE
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(531..>686)
                     /locus_tag="N7C74_005152"
     CDS             complement(531..>686)
                     /locus_tag="N7C74_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU2434794.1"
                     /translation="ADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
BASE COUNT          140 a          218 c          178 g          150 t
ORIGIN      
        1 ctgacgttcc catgtacccg cgtatcgttt catcggcgcg ggcaatggca gcatcgaaca
       61 ggttatcgaa atcagccaca gcgcctcccg ttattgcatt ctggccaggc cgcgctctgt
      121 catttcggct gccacaccgg cagagacacg aaacgccgtt cccggcagca caaatgccac
      181 aggttcatcc cgcgtgacgt gaagtgcatc agtatgcagc ttcaccagtg ccacgaccgt
      241 gaccagttca gacgtatcca gaatcacggt atccggctgc gctgatccca cctcattttc
      301 atgtccggtc agcacatttt cccggctgag aggggtgtcc tgaccggcag tttcatccgt
      361 gtcatcaagc tcctcttcca gctctgccac acggagcgcc agttcttctt tcgtccccgt
      421 caggctgaca tcacggttca gttgttcacc cagcgagcgg agacgggcaa tcagttcatc
      481 tttcgtcatg gactcctcca cagaaaaaca atggccccga agggccatga ttacgccagt
      541 tgtacggaca cgaactcatc agggtcagcc agcagcatca gcggtgctga ctgaatcatg
      601 gtgaactcac gcgccggatc gccggtggtc acccagtttt tcgggtaacg ggcagaggcg
      661 ttaatgcctt cgcgctgtgc gtccgc
//
LOCUS       DBFIFM010000527          679 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.539, whole genome shotgun sequence.
ACCESSION   DBFIFM010000527 DBFIFM010000000
VERSION     DBFIFM010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.539"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(63..569)
                     /gene="espH"
                     /locus_tag="N7C74_005153"
     CDS             complement(63..569)
                     /gene="espH"
                     /locus_tag="N7C74_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspH"
                     /protein_id="HGU2434795.1"
                     /translation="MSLSGAVFKTFLTSEHASWNRFNRRLHIPNEDIVDEIQLKARMQ
                     QRHHRVYPEIGDSTIVSFRGKDYAVHFIKDGPKDDYVYKVQRITPENGCFSTLFSVFS
                     GGVTKALERKLNERHITPLSSTWFPRTPLEGILAERGLSSLLRRVQSTERLDNRAIAT
                     RASSYSVL"
     gene            complement(600..>679)
                     /locus_tag="N7C74_005154"
     CDS             complement(600..>679)
                     /locus_tag="N7C74_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system protein SepQ"
                     /protein_id="HGU2434796.1"
                     /translation="NTTIAYGSIVKINEDFYFTVSIVCD"
BASE COUNT          207 a          141 c          120 g          211 t
ORIGIN      
        1 cggaaaaact ccagaattat aactattctg gagttaagtc aggaagaaaa ctcaaatgct
       61 aatcataata cgctataaga ggaagctctt gttgctatag ccctattatc aagacgttcg
      121 gtagactgta cacgacgtaa caaagaagat aatcctcttt cagctaagat cccctccagg
      181 ggagtacgag gaaaccaggt tgaagaaagt ggagttatat gtctctcatt taattttctt
      241 tccaaagcct tagtcacacc gccagaaaac acgctaaata gcgttgagaa acatccattt
      301 tccggtgtta ttctttgtac tttgtacaca taatcatctt ttgggccatc tttaataaag
      361 tgaacagcat agtctttgcc tctaaaagag acaatggtgg aatcccctat ttctggatat
      421 acccgatgat ggcgttgttg cattcgggct ttaagctgaa tttcatctac gatatcttca
      481 ttgggtatat gaagccttct gttgaacctg ttccatgacg catgttcact tgtcagaaat
      541 gtcttgaata ccgctcctga taacgacatg atacatctcc ctatataacg tattcctgat
      601 taatcacata ctatgctaac agtaaaataa aaatcttcgt tgatttttac aatactcccg
      661 tatgctatcg tcgtattat
//
LOCUS       DBFIFM010000528          671 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.540, whole genome shotgun sequence.
ACCESSION   DBFIFM010000528 DBFIFM010000000
VERSION     DBFIFM010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.540"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..>671
                     /gene="escC"
                     /locus_tag="N7C74_005155"
     CDS             <1..>671
                     /gene="escC"
                     /locus_tag="N7C74_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HGU2434797.1"
                     /translation="ISRSIITPTYLDIDSLLKYLSDTISVNKNSCNVRKITTFNSIEV
                     RGVPECIKYITSLSESLDKEAQSKAKNKDVVKVFKLNYASATDITYKYRDQNVVVPGV
                     VSILKTMASNGSLPSTGKGAVERSGNLFDNSVTISADPRLNAVVVKDREITMDIYQQL
                     ISELDIEQRQIEISVSIIDVDANDLQQLGVNWSGTLNAGQGTIAFNSSTAQANISSSV
                     ISNAS"
BASE COUNT          224 a          125 c          132 g          190 t
ORIGIN      
        1 aaatatcaag aagtataatt acaccaacat atctggatat tgactcactg ctaaaatatc
       61 tgagtgatac aatctcagta aacaaaaact cttgcaatgt gagaaaaatt acgacattta
      121 actcgattga agttcggggt gttcctgaat gtataaaata tattacatct ttatcagaaa
      181 gtcttgataa agaggcacag tctaaagcca aaaataaaga tgttgttaag gtctttaaac
      241 tgaactatgc gtcagcgaca gatataacat acaaataccg tgaccaaaat gttgtcgtcc
      301 caggtgttgt ctccattctg aagacaatgg caagtaatgg ttcacttccc agtaccggta
      361 aaggtgccgt cgagcgaagc ggtaacctgt ttgataacag tgtaacgatt tccgctgatc
      421 cacgtcttaa tgcagttgtt gtaaaagacc gcgaaataac gatggatatc tatcagcaat
      481 taatttctga gcttgatatt gaacaaaggc aaattgaaat ttctgtctca attattgatg
      541 ttgatgcaaa cgatcttcag cagctcggcg taaactggtc cggtacgtta aatgcaggac
      601 aaggtacgat tgcctttaat agctctaccg cacaggctaa tatcagctct tctgtgataa
      661 gtaatgccag t
//
LOCUS       DBFIFM010000529          665 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.541, whole genome shotgun sequence.
ACCESSION   DBFIFM010000529 DBFIFM010000000
VERSION     DBFIFM010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.541"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..>665
                     /locus_tag="N7C74_005156"
     CDS             <1..>665
                     /locus_tag="N7C74_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HGU2434798.1"
                     /translation="SQFNHWFGEEKPSPDLLCGYLSVPLKYTDTGGDASYEKKSQVKL
                     ALTKLPAKSKHKGSILIISGGPGLPGINPYINFDWPVTNLRESWDIIGFDPRGVGQST
                     PTINCRQSDTETQENITEKQQVLNKINACIHNTGAEVIRHIGSNEAVYDIDRIRQALG
                     DKQLTAVAYSYGTQIAALYAERFPYNVRSIVLDGVVDIDDLEDNFTWQLKQAQSYQET
                     FDR"
BASE COUNT          213 a          139 c          144 g          169 t
ORIGIN      
        1 agtcaattca accactggtt tggtgaggaa aaaccgtctc ctgacttact atgtggttat
       61 ttgtctgttc cattaaaata tacagacaca ggcggagatg cttcttatga aaaaaaatca
      121 caagtcaaac tagcgttgac aaaattgccg gcaaaaagca agcataaagg aagtatcctg
      181 ataataagtg gtggtcccgg gttaccaggc ataaatcctt atattaactt tgactggcca
      241 gtcacaaatc ttcgtgagtc atgggatatt attggatttg atcctcgagg cgtcgggcag
      301 tccactccga caataaactg ccggcaatca gatacagaga ctcaggaaaa cataaccgaa
      361 aagcaacaag tattaaataa aattaatgcc tgtatccata ataccggagc cgaagtcatt
      421 cgccatatcg gctctaacga ggctgtatac gatattgatc gtattaggca agccttgggg
      481 gataaacaac tgacagccgt ggcgtattcg tatggaactc aaattgcagc cttatatgca
      541 gaacgttttc cctacaacgt aagatctatc gttcttgatg gagtcgtcga tatcgatgac
      601 ctggaggaca acttcacatg gcaactcaaa caggcacaga gttatcagga aacgtttgat
      661 cgctt
//
LOCUS       DBFIFM010000530          657 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.542, whole genome shotgun sequence.
ACCESSION   DBFIFM010000530 DBFIFM010000000
VERSION     DBFIFM010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.542"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..>657
                     /gene="escD"
                     /locus_tag="N7C74_005157"
     CDS             <1..>657
                     /gene="escD"
                     /locus_tag="N7C74_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HGU2434799.1"
                     /translation="GTEDNDIVYFPLEQGLNQFLLDIREEGVFLLSPVEFWIDGQPTP
                     YEADKPLPVGKVIDIAGCCFIIGDIDHSLPLSDVPERFSAKSRRKKRLILASVIGATF
                     ALSGAIGSYVLLSPKAEPPTFTRADVYQQLKENKLHAITLVWHGKNIALYGRCESTTD
                     LTPFFNYLKEKNIFYYNNIICNNQIISAINDVLTEYGYKDIIITKGNKPGFFLLSGYI
                     P"
BASE COUNT          192 a          133 c          128 g          204 t
ORIGIN      
        1 ggtacggaag ataacgatat tgtttacttt ccccttgaac aaggcctcaa tcagttttta
       61 cttgatatac gtgaggaagg tgttttcctt ttatctccgg ttgaattttg gattgatggg
      121 caacccacgc cctatgaagc agataaacca ttacctgtcg gaaaggttat tgatatagcg
      181 ggatgttgct tcatcattgg tgatattgac cattccttgc cattgtctga tgtgccagaa
      241 aggttctctg ccaaaagtcg acgcaaaaaa cgccttatcc tggcgagcgt gattggtgct
      301 acttttgcgt tgtctggtgc catcggcagt tacgttttgc tttccccaaa agctgaaccc
      361 ccaacgttta ccagagctga tgtatatcag cagctaaaag aaaacaaact tcatgcaatc
      421 actctggtat ggcatgggaa aaatatcgcg ctttatgggc gttgcgaatc aaccacggat
      481 ttaactccat tttttaatta cctaaaagaa aaaaatattt tttattacaa taatatcatt
      541 tgcaataacc aaatcatatc cgcaattaat gatgtcttaa cggaatacgg ttacaaagat
      601 atcattatta caaaaggtaa taagcctgga ttctttctgt tatcgggata cattccc
//
LOCUS       DBFIFM010000531          656 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.543, whole genome shotgun sequence.
ACCESSION   DBFIFM010000531 DBFIFM010000000
VERSION     DBFIFM010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.543"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..>656
                     /locus_tag="N7C74_005158"
     CDS             <1..>656
                     /locus_tag="N7C74_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU2434800.1"
                     /translation="NIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELNTSLKFV
                     IERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSNVNRTIA
                     NVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRDVTEEEG
                     NIVIYTEPTDLHKGIAALKAAGISEFSTTELEMIAQSEVELSPEDLEIFEGLVDALED
                     "
BASE COUNT          216 a          115 c          144 g          181 t
ORIGIN      
        1 aatattgttg ctaaaaaaac ggctaaagac ggtgcaacgt ctaaaattta tgcaaaattc
       61 ggtgtagaaa tctatgctgc cgctaaacaa ggtgaacccg atccagaatt aaacacatct
      121 ttaaaattcg ttattgaacg tgcaaagcag gcacaagttc caaagcacgt tattgataaa
      181 gcaattgata aagccaaagg cggcggagat gaaacattcg tgcagggccg ttatgaaggc
      241 tttggtccta atggctcaat gattatcgcc gagacattga cttcaaatgt taaccgtacg
      301 attgctaacg ttcgcacaat tttcaataaa aaaggcggca atatcggagc ggcaggttct
      361 gtcagctata tgtttgacaa tactggtgtg attgtattta aaggatcaga ccctgaccat
      421 atttttgaaa ttttacttga agctgaagtt gatgttcgtg atgtgactga agaagaaggt
      481 aacattgtta tttataccga acctactgac cttcataaag gaatcgcggc tctaaaagca
      541 gctggaatca gtgagttctc aacaacagaa ttagaaatga ttgctcaatc tgaagttgag
      601 ctttcaccag aagatttaga aatctttgaa gggcttgttg atgcccttga agatga
//
LOCUS       DBFIFM010000532          646 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.544, whole genome shotgun sequence.
ACCESSION   DBFIFM010000532 DBFIFM010000000
VERSION     DBFIFM010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.544"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
BASE COUNT          212 a          137 c          132 g          165 t
ORIGIN      
        1 tagatctcat tgcataaaca aataaaacag aataaaaacc aagctgaacc gtttcactat
       61 actctactta attcgagtta caggaaggcg gcaagcgagt gaatccccag gagcttacat
      121 aagtaagtga ttggggtaag cgaacgcagt cgcagcacat gcaacttgaa gtatgacgag
      181 tatatatatc tattagctgt ttcccggaca taagctacag ccagcactaa cctggaacgg
      241 gtgcattata aaaaggaaca agggacaaat agctgaacag taaccgcgtt agtctcttcc
      301 cgcataaccg taccattcgt attaagaagt attgcccata tccattcggc gaaagaggat
      361 ctgccataca tcaagtccgt tttacttggc atcagatcat cgccgtcatt aactctgtta
      421 aagtcggaca aacgattagc aagatctgtg attcaaaagt gcttagcgct aaggtgggac
      481 tggttacaga ttcattaacc ctgttctttg tcgcacctat cgatcggtgt aatgccacaa
      541 caggtttatg ttaatcagaa caatgcgccc ctctataaag agaattcttt gataaggcaa
      601 cttgaaaagg gaaagctaac tgatatcaat gtaccacttt agtaat
//
LOCUS       DBFIFM010000533          639 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.545, whole genome shotgun sequence.
ACCESSION   DBFIFM010000533 DBFIFM010000000
VERSION     DBFIFM010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.545"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            75..639
                     /locus_tag="N7C74_005159"
                     /pseudo
     CDS             75..639
                     /locus_tag="N7C74_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          179 a          144 c          115 g          201 t
ORIGIN      
        1 taactcatta taaataatga taaaataacg tcttttattg ataatttaca tatgaaaaaa
       61 attttccttt tgttatatta tttctgtttt cactccctct gattattttt taccaaccct
      121 gggtcactgc attatcgcca acgccccgac acgtaattcc tgagcaatta gaaaaaacag
      181 ttcgttatct tacacaaact gtgcatccac gtaatgccga caatattgat aatctgaata
      241 ggtccgtaga gtatataaaa aaaggtcttt gtcagtagcg gtgccagagt taccgcgcag
      301 ggcgtcccca attggctatt acgactctgc gcctggtagc caggattacc cttacccgca
      361 atgtcctggc tttatcccga tcggggggac tttattgcct ggtcggcaga atacaggata
      421 tcaacgccgt tcgtcaggta aaaacggcat tgttatcatc ccgggattta tctgtttatt
      481 ctatgaatgc cccatggttt attcacggta ttgatttctc tgaccacctt aattattggc
      541 aacacgatat tctcgccgta atgattactg acaccgcttt ttatcgtaat aaacaatacc
      601 acttgcccgg tgatacagca gacagattga attatcaga
//
LOCUS       DBFIFM010000534          635 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.546, whole genome shotgun sequence.
ACCESSION   DBFIFM010000534 DBFIFM010000000
VERSION     DBFIFM010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.546"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..609
                     /locus_tag="N7C74_005160"
     CDS             <1..609
                     /locus_tag="N7C74_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434801.1"
                     /translation="FLLSGNAGEMILYFANVFFNPLALSFIIATIICITIKKRSSRSF
                     IRGTCWLMGLFLIYSSYTVWERHSIWDYTFPEPGITVTVPSKQWVANIVKQGPTLVTR
                     DAHAILAIVAFSQNELGVHSIEELTATQNGTAEMHVCDVQGFACAYQEGVQTMSDGKV
                     RHAIFMTLLDNTNLIQLVAAIDPDYLDEYQEEVMKMMLSARQ"
BASE COUNT          186 a          125 c          137 g          187 t
ORIGIN      
        1 tttcttttat ctggcaacgc gggtgaaatg attttatatt ttgctaacgt attttttaac
       61 ccacttgcct tatcatttat tatcgccaca atcatctgta taacgataaa aaaaagaagc
      121 agccgcagct ttattcgcgg cacttgttgg ttaatgggat tatttcttat ctatagcagc
      181 tatacggtgt gggaacgaca cagcatatgg gattatactt tcccggagcc aggtatcacc
      241 gtcacggttc cgagcaaaca gtgggttgcc aatatagtaa aacaaggacc aaccctggtg
      301 acccgggatg cccatgctat tttagctatt gttgccttct cacaaaatga actcggtgtg
      361 cattcaatcg aggaacttac cgcgactcaa aacggaactg cagaaatgca tgtttgtgat
      421 gttcagggat ttgcctgcgc ttatcaggaa ggagtgcaaa caatgagtga tggcaaagta
      481 agacatgcta ttttcatgac tctactggat aacactaatt tgatacaact ggtcgcagct
      541 atcgacccag attatttgga cgaatatcaa gaagaagtaa tgaaaatgat gctttctgct
      601 cgtcaatgat ctgatgtgca tgttaataat aatgg
//
LOCUS       DBFIFM010000535          632 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.547, whole genome shotgun sequence.
ACCESSION   DBFIFM010000535 DBFIFM010000000
VERSION     DBFIFM010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.547"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(12..623)
                     /gene="map"
                     /locus_tag="N7C74_005161"
     CDS             complement(12..623)
                     /gene="map"
                     /locus_tag="N7C74_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector Map (Rho
                     guanine exchange factor)"
                     /protein_id="HGU2434802.1"
                     /translation="MFSPMTMAGRSLVQATAQTLKPAVTRAAMQAGTGATGMRFMPVQ
                     SNFVINHGKLTNQLLQAVAKQTRNGDTQQWFQQEQTTYISRTVNRTLDDYCRSNNSVI
                     SKETKGHIFRAVENALQQPLDMNGAQSSIGHFLQSNKYFNQKVDEQCGKRVDPITRFN
                     TQTKMIEQVSQEIFERNFSGFKVSEIKAITQNAILEHVQDTRL"
BASE COUNT          153 a          148 c          126 g          205 t
ORIGIN      
        1 caaggttatc cctacaatcg ggtatcctgt acatgctcta aaatcgcatt ctgagtaatc
       61 gcttttatct cactgacttt gaagccgcta aagttgcgtt caaatatttc ttgtgagact
      121 tgttctatca tcttcgtctg ggtgttaaaa cgtgttattg gatccactct tttgccgcac
      181 tgctcatcaa ctttctgatt aaagtattta ttggactgca agaagtgacc aattgatgat
      241 tgtgccccgt tcatatcaag aggttgctgt aacgcatttt caacagctct aaaaatgtga
      301 cctttagtct ccttactaat gaccgaatta ttactcctgc aatagtcatc cagagttcta
      361 tttactgttc tggatatata agtcgtctgc tcctgctgga accattgttg ggtatcgcca
      421 ttacgagttt gtttggctac agcctgcagg agttggttag tcagtttgcc atgattaatc
      481 acaaagttcg actgcacggg catgaacctc atacccgtgg ctcccgtacc cgcttgcatt
      541 gcagctctgg taaccgcagg tttaagcgtc tgtgcagtcg cctgaactaa cgatctgcct
      601 gccattgtca ttggactaaa cataccataa ac
//
LOCUS       DBFIFM010000536          630 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.548, whole genome shotgun sequence.
ACCESSION   DBFIFM010000536 DBFIFM010000000
VERSION     DBFIFM010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.548"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..457)
                     /locus_tag="N7C74_005162"
     CDS             complement(<1..457)
                     /locus_tag="N7C74_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU2434803.1"
                     /translation="MKLSIDISELIQLGKKMLPEGVDFFLDESPIDFDPIDIELSTGK
                     EVSIEDLDPGSGLISYHGRQVLLYIRDHSGRYDAAIVDGEKGKRFHIAWCRTLDEMRH
                     KNRFERYHATNRIDGLFEIDDGSGRSQDVDLRVCMNCLERLNYKGSIDKQ"
     gene            complement(461..>630)
                     /locus_tag="N7C74_005163"
     CDS             complement(461..>630)
                     /locus_tag="N7C74_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434804.1"
                     /translation="ATHQQWVKSKFAAVGYSSAHPILDKTGKEDPNRSRRVTFKVVTN
                     AELQIRKIIQE"
BASE COUNT          185 a          141 c          111 g          193 t
ORIGIN      
        1 gttgtttatc aatacttcct ttataattaa gtcgttcgag gcaattcata catacccgta
       61 aatcaacatc ctggctccga cctgaaccat catcaatttc gaataaacca tctatgcggt
      121 tagttgcatg atacctttca aatcgatttt tatggcgcat ttcatcaaga gttctgcacc
      181 aggcaatatg aaaacgtttt cctttttcgc catctacgat agccgcatca taacgccctg
      241 aatggtcccg aatatataaa agaacctggc ggccatgata agagataagc ccgctaccag
      301 ggtcaagatc ttcgatacta acttctttac ccgtggataa ctcaatatct ataggatcaa
      361 agtcaatagg ggattcatcc agaaaaaaat cgactccttc tggtaacatt ttcttcccta
      421 attgaataag ttctgaaatg tcgatagata atttcatctc ttactcctga ataatctttc
      481 taatctgcaa ctcggcattt gttacaactt tgaaggtgac acgacgagag cgattaggat
      541 cctccttgcc ggttttatca agaatgggat gtgcagatga ataacctact gcggcaaatt
      601 tacttttaac ccattgttgg tgtgtcgcga
//
LOCUS       DBFIFM010000537          621 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.549, whole genome shotgun sequence.
ACCESSION   DBFIFM010000537 DBFIFM010000000
VERSION     DBFIFM010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.549"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..>621
                     /locus_tag="N7C74_005164"
     CDS             <1..>621
                     /locus_tag="N7C74_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HGU2434805.1"
                     /translation="GNGTFYVSGPPVYVDLVVNAAKLMEQNSDGIEIGRNKVGIIHLV
                     NTFVNDRTYELRGEKIVIPGMAKVLSTLLNNNIKQSTGVNVLSEISSRQQLKNVSRMP
                     PFPGAEEDDDLQVEKIISTAGAPETDDIQIIAYPDTNSLLVKGTVSQVDFIEKLVATL
                     DIPKRHIELSLWIIDIDKTDLEQLGADWSGTIKIGSSLSASFNNSGS"
BASE COUNT          205 a          107 c          137 g          172 t
ORIGIN      
        1 ggcaatggta ctttctatgt atcggggcca ccggtttatg ttgacctggt cgttaatgct
       61 gcgaaattaa tggagcaaaa cagcgatggc attgagatcg gtcgaaataa agtaggaata
      121 attcatctgg tcaacacatt cgttaatgac cggacttatg agttacgtgg cgaaaagata
      181 gttattcctg gcatggcaaa agtactatcg acgttgttaa ataataacat taagcaaagc
      241 acaggggtga acgtactttc tgaaatttca agtcgtcaac aactaaaaaa cgtatcacgt
      301 atgcctcctt ttcctggggc tgaagaagat gatgacctac aagtagagaa aataatatcg
      361 acagcaggag cgccagagac tgacgatata caaattattg catatcctga taccaacagt
      421 ttacttgtta aaggaacagt atctcaggtt gattttatcg agaagttagt agctacgctt
      481 gatattccaa agcgacacat tgaattatct ttatggataa tagatattga taagactgat
      541 ctagaacagc taggtgctga ctggtcgggc acgataaaaa ttggatccag cttaagtgca
      601 tcatttaata attcaggttc a
//
LOCUS       DBFIFM010000538          620 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.550, whole genome shotgun sequence.
ACCESSION   DBFIFM010000538 DBFIFM010000000
VERSION     DBFIFM010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.550"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(92..>620)
                     /locus_tag="N7C74_005165"
     CDS             complement(92..>620)
                     /locus_tag="N7C74_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU2434806.1"
                     /translation="KYIIPFVATTLMFSATTFAKDVDQGTLTIRGEVVDTTCYFVNGD
                     STTDIVVEDVVTSAMNQLINNEIYTLKTGSTSHDLEIQCDGNTAPSIEVLASEFDANN
                     FTLNKGTAENIGFAIFLDDGTLTRMRPGLKTPLKDNGSGQYFLNLNAQYARTSGNPVT
                     KGSVESTVTIKISAD"
BASE COUNT          182 a          123 c          117 g          198 t
ORIGIN      
        1 taaaataaaa aatgacatca gtaatgctgt tattattctc acgtaatcac tccgtaaata
       61 ataccggtag cagagctacc ggcaaaaaac gttaatctgc actgatttta atcgttacgg
      121 ttgactctac ggagcctttt gtaacaggat tgccacttgt gcgggcatat tgtgcattta
      181 aattgagaaa atattgacca gagccattat ctttcagcgg tgtttttaac cctggtctca
      241 ttctggtcaa agtcccatca tcgaggaaaa tggcaaagcc aatgttctca gcagttcctt
      301 tattcagcgt aaagttatta gcatcaaact ctgatgctag tacttcgata cttggggctg
      361 tatttccgtc acactgaatt tctaagtcat gagaggttga accggttttt aaagtataga
      421 tttcattatt gataagttga ttcattgcac tggttacaac atcttcgaca acgatatctg
      481 ttgtcgaatc accattaaca aagtaacacg ttgtatcaac aacttctcct ctaatggtta
      541 gtgttccttg atcgacatcc ttggcgaagg ttgttgcgct aaacatcaac gttgtcgcaa
      601 cgaagggaat aatatacttt
//
LOCUS       DBFIFM010000539          617 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.551, whole genome shotgun sequence.
ACCESSION   DBFIFM010000539 DBFIFM010000000
VERSION     DBFIFM010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.551"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..127
                     /locus_tag="N7C74_005166"
     CDS             <1..127
                     /locus_tag="N7C74_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU2434807.1"
                     /translation="PKDTTQVQGLFRHLQVRFGDGPWQDVKGLNEVGSDTGRTGE"
     gene            129..398
                     /locus_tag="N7C74_005167"
     CDS             129..398
                     /locus_tag="N7C74_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU2434808.1"
                     /translation="MNILKKLMQRLCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
BASE COUNT          178 a          121 c          174 g          144 t
ORIGIN      
        1 ccccaaagat accacacagg ttcaggggct gtttcggcat ttgcaggtca ggtttggtga
       61 cgggccgtgg caggatgtta aggggctgaa tgaagtgggc agtgatacag gcagaacagg
      121 agaatgacat gaatatacta aaaaaactta tgcagcgtct gtgtggttgc ggaaagcatg
      181 atggccgtga acacgtgcag tcgcttacag cacaactgcg actggggccg gcagacatcc
      241 tggagtccga tgagaatggt attattccgg agcaggacag ggtaatcacg caggtggtga
      301 tactggatgc ggataaaaag cagatacagt gcgtggtaag accgctgcaa attctgcgtg
      361 ctgacgggac gtgggaaaat attggcggaa tgaaatagcc gacagcttca caaaaaccgg
      421 agtccggctc cggtttttgt tgtcatgtcc ggtggatgtt tgttaggaat gttcagacag
      481 gtttattttg aatttacaca gaatcctaaa caggttcgaa aattaagaaa gaggttgtat
      541 gttttgcata agaaccctac tacctattag cgccagcgta tcagttccga caaaacaatc
      601 tcaatccatc ccaataa
//
LOCUS       DBFIFM010000540          614 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.552, whole genome shotgun sequence.
ACCESSION   DBFIFM010000540 DBFIFM010000000
VERSION     DBFIFM010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.552"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            235..310
                     /locus_tag="N7C74_005168"
     tRNA            235..310
                     /locus_tag="N7C74_005168"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:268..270,aa:Ile,seq:cat)
BASE COUNT          178 a          104 c          141 g          191 t
ORIGIN      
        1 atacaaaatc attacaaagt atcattgaat cgatgataac tatagattgt gctaattatt
       61 aacatttgct tgttgactgc gagtatcgac tttgcggaat ggaggtggtg tcgggtttta
      121 tgtcgcatgt attgacgacc caggatggat gagaacgcat aaaaatctgt gatttacatc
      181 ataagatcat catgtttatt gcgtgggtta taagtattgt tattgcatac ttaaggccct
      241 ttagctcagt ggttagagca ggcgactcat aatcgtccgg tcgctggttc aagtccagca
      301 agggccacca aatcatcatt catgtatcag gaagagttaa gggtacaagg gttactcgat
      361 ggcggtctat atttcacgat taacgccatt tcaaggaaga agtgcggtgg gtggtttcga
      421 atgagggctg gtgttttggt acgtggcacg ccactgcaat gtatttatct atcagtactc
      481 attcaaaagt atttggaaaa aatgcctgct atgccaagcg ggcaaattgc ttacatgtgg
      541 aattaagtaa ttaacgataa agccagcgta ttatcattta ttatacccat tcatttaatc
      601 aggttaatta ctca
//
LOCUS       DBFIFM010000541          606 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.553, whole genome shotgun sequence.
ACCESSION   DBFIFM010000541 DBFIFM010000000
VERSION     DBFIFM010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.553"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..139)
                     /locus_tag="N7C74_005169"
     CDS             complement(<1..139)
                     /locus_tag="N7C74_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU2434809.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GG"
     gene            201..606
                     /locus_tag="N7C74_005170"
                     /pseudo
     CDS             201..606
                     /locus_tag="N7C74_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          172 a          152 c          138 g          144 t
ORIGIN      
        1 tgccgccagt atcccttaac atagtgaaga tcgttccggg ttttgaaccc aggatattcg
       61 ctatttcact gaagcctgtt ccgttcttcc atagttcaaa aacagaggct ttttcctctg
      121 ctgtaaatgt tcgtctcatt caaaaaacct ccgcaacccc atgttttcac ataactgttg
      181 cgttgaccaa ttgaatctac aacagcgtat tttgcacagg agtcgctgaa aaatacgcgt
      241 taatcgaaca atggcgacaa caatttccca ttgaagcgat gtgtcaggta tttggtgtat
      301 ccaggagcgg ttattacaac tgggtacagc atgaaccctc agacagaaaa caaagtgatg
      361 agcggctaaa actggagatt aaggtggcac atatccgcac tcgcgaaaca tatggaaccc
      421 ggcggctcca gacggagctg gcagagaatg gcatcatcgt tggtcgtgac cgactggcac
      481 gtcttcgtaa ggagctaagg ctacgctgta agcagaaacg caagttcagg gcgactacga
      541 actcgaacca caatctgcca gttgcgccaa atctgctgaa ccagacgttc gctcctacag
      601 caccaa
//
LOCUS       DBFIFM010000542          598 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.554, whole genome shotgun sequence.
ACCESSION   DBFIFM010000542 DBFIFM010000000
VERSION     DBFIFM010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.554"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            58..>598
                     /locus_tag="N7C74_005171"
     CDS             58..>598
                     /locus_tag="N7C74_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGU2434810.1"
                     /translation="MNIYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGAD"
BASE COUNT          199 a          116 c          149 g          134 t
ORIGIN      
        1 caaggcggag tatcaacaga aacttaaaaa actgcgaaac agcagaagtg aggttgcatg
       61 aatatctacg aaagaattga tggcagcaaa taccgaaata tttgggtagt tggcgatctg
      121 cacggatgct acacgaacct gatgaaaaaa ctggagacga taggattcga caccaaaaaa
      181 gacctgctta tctcggtggg cgatttggtt gatcgcggta cagagaacgt agaatgcctg
      241 gaattaatca cattcccctg gttcagagct gtacgtggaa accatgagca aatgatgatt
      301 gatggcttat cagagcgtgg aaacgtcaat cactggctgc ttaatggcgg tggctggttc
      361 tttaatctcg attacgacaa agaaattctg gctaaagctc ttgcccataa agcagatgaa
      421 cttccgttaa tcatcgaact ggtgagtaaa ggaaaaaaat atgtcatctg ccacgccgat
      481 tatccttgtg ataaatacga gtttggaaag ccagttgatc atcagcaggt aatctggaac
      541 cgcgaacgaa tcagcaactc acaagacggg atcgtgaaag aaatcaaagg cgcggaca
//
LOCUS       DBFIFM010000543          597 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.555, whole genome shotgun sequence.
ACCESSION   DBFIFM010000543 DBFIFM010000000
VERSION     DBFIFM010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.555"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            356..>597
                     /locus_tag="N7C74_005172"
     CDS             356..>597
                     /locus_tag="N7C74_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434811.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQA"
BASE COUNT          163 a          107 c          161 g          166 t
ORIGIN      
        1 tgaagttgtt tttacgttaa gttgatgcag atcaattaat acgatacctg cgtcataatt
       61 aattatttga cgtggtttga tggcgtagat gcacgttgtg acatgtagat gataattatt
      121 atcattttgc gggtcctttc cggcgatccg acaggttacg gggcggcgac ctcgcgggtt
      181 ttcgctattt atgaaaattt tccgggatcc atgtccggtt tctcttcaag ttaactatat
      241 gaaaaatata aaaacaggtc ttctgtgaac cggacatgaa caaaaaacag acatgtaaac
      301 cggacatgac cggttttgtt gtgattgtga ggtgagagtt tttgcgaggt gaggagtggc
      361 tacgcagact gaagttgcca ggcatttaag tctgaccgat cgccagcttc gcagattgca
      421 gaaattgccg ggtgccccga tatcgaataa gcgagggcaa ctggatctgg atgcctggcg
      481 cgatttttac atatcgtatc tgaggagaag taaaaacgat gtgcctgatg gcgatagcga
      541 agacgactat gaggagaaat tgcttattgc cagatgggaa ctgacagcag aacaggc
//
LOCUS       DBFIFM010000544          593 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.556, whole genome shotgun sequence.
ACCESSION   DBFIFM010000544 DBFIFM010000000
VERSION     DBFIFM010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.556"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            53..418
                     /locus_tag="N7C74_005173"
     CDS             53..418
                     /locus_tag="N7C74_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU2434812.1"
                     /translation="MAVLRTLPGRIKTLNTRRINVLRGEQRRVSGSARVSLKRRIWRR
                     DAGHCCLCRRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSASEAASGMPD
                     PTLPELPDGTLRADGITGL"
BASE COUNT          130 a          147 c          168 g          148 t
ORIGIN      
        1 ttcgatgaca ttttctttcc tctggtgaca agctatatgg cgaggatttt acatggctgt
       61 gcttcgtacg ttaccgggca gaatcaaaac tctgaacacc cggcggataa atgtcctgag
      121 gggggaacag cgtcgtgtca gtggcagtgc ccgggtttcc ctcaaacgtc gtatctggcg
      181 gagggatgcc ggacactgtt gtctctgtag acgcgtggtt gacctttgtg acagtgaact
      241 cgatcaccgc attgcacttc agttcggtgg tggtaatgag gagacgaacc tctggaccct
      301 ctgtactgaa tgccatcgcc agaagtcagc gagtgaagcg gcgagtggta tgcctgatcc
      361 gacgttgcct gagcttcctg atggcacgct cagggccgac ggaatcactg gcctgtgacc
      421 agacccgggg gggatcatcc ggcgaaaaaa aacgatcgcc ctggacaccg cgccccctct
      481 cacgcagaga aaaaattccc gtttcagggc agttaacatg ttaactggct gcccgggcat
      541 ttttgcggtt tttatcttta ttattcagtt tgttgtgcgg aaaaaatgtt aac
//
LOCUS       DBFIFM010000545          592 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.557, whole genome shotgun sequence.
ACCESSION   DBFIFM010000545 DBFIFM010000000
VERSION     DBFIFM010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.557"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(6..313)
                     /locus_tag="N7C74_005174"
                     /pseudo
     CDS             complement(6..313)
                     /locus_tag="N7C74_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          146 a          122 c          132 g          192 t
ORIGIN      
        1 cgcagttatc tggttatatt tttcctgaag gctaccaggg agatattccc cagacgggag
       61 tggcggcgct gatggttgta gaaaatctca atgtatttcc gtattactta gatggcttca
      121 tcccggttat taaaacgata gtttcattct tcagcgttta ctataagttt ttcattgagg
      181 tgttgtcgta acggttacct ttacgcggca tggatgtttt cagaccaaac cgctcctgta
      241 tagcccggta atcgtgtgtg cagtactgcg aaccacgatt ggagtggtgg attagcccga
      301 ctggtgggcg ttgcatttcc cggtgttacc atacgttttt gctgcccacg tagtttcctg
      361 tccgtaccga ttttgagatt tttcctgata tcctcaatgc ccccttgtat aacaaagata
      421 ctgttaaaaa ccattgttat gtattacctt tttcccatta tggggtgcta atatttcaag
      481 aggaaataat accccctaaa gtatcctcaa aagcaacttg atacaggttg agtcggttga
      541 cccctgtaca caaagaaagc ctgatatata agggattaaa aggggttttg tt
//
LOCUS       DBFIFM010000546          576 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.558, whole genome shotgun sequence.
ACCESSION   DBFIFM010000546 DBFIFM010000000
VERSION     DBFIFM010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.558"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            27..305
                     /locus_tag="N7C74_005175"
     CDS             27..305
                     /locus_tag="N7C74_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434813.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRSAIELDI
                     DWPESNDDLWKDGEEGAYAMGYEDGRDKTVIAVMKAIRAAGIKEKNFD"
     gene            438..>576
                     /locus_tag="N7C74_005176"
     CDS             438..>576
                     /locus_tag="N7C74_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434814.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPLDELYEHSPAARRYP
                     RD"
BASE COUNT          167 a          101 c          166 g          142 t
ORIGIN      
        1 ggccttttta tttgggggag agggaagtga acgatagcta ccgacagttt gaaaactggt
       61 ggtcaaaaga caaaagccag ttcacgggag acgatgaatt aaaagagttt gcctgggtga
      121 tatggcaggc atcgcgctct gctattgaac tggatatcga ctggcccgaa tcgaatgacg
      181 acctttggaa agatggtgaa gaaggtgctt atgcgatggg ttatgaggat gggcgtgaca
      241 aaacggtaat tgcagtaatg aaagccatca gggccgcagg aatcaaagaa aagaatttcg
      301 attaagcaaa tatcacttca ataaatcgct tttaaggcat cacaatcgct ctgtggtgag
      361 gtaagcacgt gcaaggtata ccgataagca gcgagaatga aaaatgcgtc agaatgcgtt
      421 tgaggaggtt ttaagaaatg agtacgatag ctgagcttgt cagggctaat tttcgtgaag
      481 agttggtgcg ttggtatcgg tatcgttcat cgtccagttt gccgcttgat gagttgtatg
      541 agcattcacc tgccgcacga cgctatccgc gtgacc
//
LOCUS       DBFIFM010000547          558 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.559, whole genome shotgun sequence.
ACCESSION   DBFIFM010000547 DBFIFM010000000
VERSION     DBFIFM010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.559"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..298
                     /locus_tag="N7C74_005177"
     CDS             <1..298
                     /locus_tag="N7C74_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Replication protein O"
                     /protein_id="HGU2434815.1"
                     /translation="FDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHRDMCVLFRWA
                     CQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTAGKSKLDLTNTDWIYGVDL"
     gene            295..>558
                     /locus_tag="N7C74_005178"
     CDS             295..>558
                     /locus_tag="N7C74_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU2434816.1"
                     /translation="MKNIAAQMVNFDCEQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     RQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAG"
BASE COUNT          160 a          143 c          153 g          102 t
ORIGIN      
        1 gtttgacatg gtgaagacca tcgcgccatc agccagaaaa ccgaattttg cagggtgggc
       61 taacgatatc cgcctgatgc gtgaacgtga cggacgtaac caccgcgaca tgtgcgtgct
      121 gttccgctgg gcatgccagg acaacttctg gtccggtaac gtgctaagtc cggccaaact
      181 ccgtgacaag tggacccaac tcgaaatcaa ccgtaacaag caacaggctg gagtgacagc
      241 cggaaaatca aaactggacc tgacaaacac tgactggatt tatggggtgg atttatgaaa
      301 aacatcgccg cacaaatggt taactttgac tgtgagcaga tgcgccggat cgccaacaac
      361 atgccggaac agtacgacga aaagccacag gtacagcagg tagcgcagat catcaacggt
      421 gtgttcaggc agttactggc aactttcccg gcgagcctgg ctaaccgtga ccagaatgaa
      481 ctgaacgaaa tccgccgcca gtgggttctg gctttccggg aaaacgggat caccacaatg
      541 gaacaggtta acgcagga
//
LOCUS       DBFIFM010000548          554 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.560, whole genome shotgun sequence.
ACCESSION   DBFIFM010000548 DBFIFM010000000
VERSION     DBFIFM010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.560"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(336..470)
                     /locus_tag="N7C74_005179"
     CDS             complement(336..470)
                     /locus_tag="N7C74_005179"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU2434817.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
BASE COUNT          157 a          144 c          109 g          144 t
ORIGIN      
        1 ctcagtaaaa aatcagaaga ttatcatcct gatttattgc ataaaaccag tcgagtagcg
       61 gaattttctg catccggata ccactccccc tgaaaaatac tcgcacaata tcgcgccgca
      121 tgcactgatg gaccgggcca tcgtgagaat ctcttgcatc gtagatatta accacaccag
      181 tgccccccag aacaacagta atacctgtag cagacctgcc atagaggtta aataaccatt
      241 catatccctc ttctacccat tcatcccgaa gtttcagcca gcgacgaatg gactgtacct
      301 cctgtttcag ccgaggggct gagttgacaa accgtttaga gccgccggat attcaggtta
      361 ccggcctgca aatcaatatc agacagtttt atacctaata actctgatgc ttggacacca
      421 tgaagatacc ccataaggag caggcagtga ttacgttccg ggtgtggcac tttgaagaca
      481 gcactgtgca acttatgaat ctcacccggg ttcagatatt tttgttgtcc attacctgtc
      541 cttatttatt gaaa
//
LOCUS       DBFIFM010000549          548 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.561, whole genome shotgun sequence.
ACCESSION   DBFIFM010000549 DBFIFM010000000
VERSION     DBFIFM010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.561"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            468..>548
                     /gene="ykgL"
                     /locus_tag="N7C74_005180"
     CDS             468..>548
                     /gene="ykgL"
                     /locus_tag="N7C74_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
                     /protein_id="HGU2434818.1"
                     /translation="MQAASQREETEWRVQSKRGLMPAYRGE"
BASE COUNT          166 a          100 c          137 g          145 t
ORIGIN      
        1 aactttgccc tgaggtgtta ggaatagtct taatgtgttg tgtaatggac tagtaaaaaa
       61 acaagtctaa atgataagaa atagaatatg aaactatcat gagtcataat taaagatgat
      121 ttgccaatca gtaaggtgtt ttaatggatt aacatgccgc acaaaatggt tatgggtgtt
      181 catcccaata agtgatgaaa aattaaacag gtgtaagtac gtcatctgtc cggtaagccc
      241 cctaagagcc agattaagtg aagttaaacg aagactgcca gttgtcaccg gaacgatggc
      301 ctctttgcca gggcactgca ttcagtcatc tgtgcgcgtt actctaactg gcactcgaac
      361 gtcggcgacg gggttaccgg tggatcaggc cgttcctgtg gcgtaaacgt ttttatgtca
      421 gtaccaacag atttaccaca tttatcatct taacagtttg agtgtaaatg caggctgcgt
      481 cgcaaaggga ggaaactgag tggcgtgttc agtctaaaag agggttgatg ccagcataca
      541 ggggcgaa
//
LOCUS       DBFIFM010000550          547 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.562, whole genome shotgun sequence.
ACCESSION   DBFIFM010000550 DBFIFM010000000
VERSION     DBFIFM010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.562"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            255..470
                     /locus_tag="N7C74_005181"
     CDS             255..470
                     /locus_tag="N7C74_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU2434819.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            475..>547
                     /locus_tag="N7C74_005182"
     CDS             475..>547
                     /locus_tag="N7C74_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HGU2434820.1"
                     /translation="MKKKYELGVKGINNYPDKITVTVA"
BASE COUNT          135 a          106 c          160 g          146 t
ORIGIN      
        1 ctggctaaga aggatagttg ggtttcacat gatacttata tctggcagta cattttctga
       61 cagacagtga tgggtgttgt caagatattg tgtcatttat aacctgaatc agggggggga
      121 gccggaatgt tatctggcat ttttagcaga gcctgaatgc cataatcacg gctcccggcg
      181 ttggccgtca gtgggcgaca ctggcggctt tttgttttcc tttactttca ttttctgtcg
      241 gcggtgacgg agacatacat cagatggaaa aaatcacaac aggtgtgtca tacaccacgt
      301 cagcggtggg gacgggatac tggttactgc agctgctgga caaagtctct ccgtcccagt
      361 gggtggcaat cggtgtgctg gggagtctgc tgtttggcct gctgacgtat ctgactaacc
      421 tgtatttcaa aatcagagag gaccgtcgta aggcggcgcg gggggagtaa agcgatgaag
      481 aaaaaatacg aactgggtgt taaagggata aataattacc cggataagat tactgttact
      541 gtggcac
//
LOCUS       DBFIFM010000551          544 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.563, whole genome shotgun sequence.
ACCESSION   DBFIFM010000551 DBFIFM010000000
VERSION     DBFIFM010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.563"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..>544
                     /locus_tag="N7C74_005183"
     CDS             <1..>544
                     /locus_tag="N7C74_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU2434821.1"
                     /translation="LTTDLLLWRSSWPTLATAIRQFSQGIVSNEIETALSAPIASEES
                     SDASGVILCVDQGDEQLTPEERKFRKEALANAFPAINFDNGRSDSPDFCELWPIHSDL
                     NKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTINGDGHTLALTGVNL
                     CVDKAVVHHLITPQKIENIY"
BASE COUNT          165 a          125 c          113 g          141 t
ORIGIN      
        1 cattaacaac agaccttctg ctatggcgtt catcatggcc aacccttgca actgccatac
       61 gccagttctc tcaggggatt gtcagtaatg aaattgaaac tgcgctcagt gctccgatag
      121 cctcagaaga gtcaagtgat gcttcggggg taatcctttg tgtagatcag ggggatgagc
      181 aattaacacc agaagagcga aaattccgaa aagaggctct tgcgaatgcc ttcccggcta
      241 ttaactttga caatggacgt tccgattcac ctgatttttg tgaattatgg ccaatacata
      301 gcgacctgaa caaaactcgc ctgaaaaata ctgttctgcc ctctggttta ctgtttgtag
      361 cacacaaata cgacccaaca acgccctgga ttaatgcccg taagatggca gagaaatttt
      421 ccagcccgtt actaacaata aatggtgatg ggcatacatt agctctcacc ggagttaatt
      481 tatgtgtaga taaagcagtt gtacatcacc tgatcactcc acaaaaaata gaaaatatat
      541 actg
//
LOCUS       DBFIFM010000552          528 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.564, whole genome shotgun sequence.
ACCESSION   DBFIFM010000552 DBFIFM010000000
VERSION     DBFIFM010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.564"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
BASE COUNT          139 a          112 c          125 g          152 t
ORIGIN      
        1 gaacattgca agggctcaca actagtagtg ttgagaaagc catcgggaaa atgaggctaa
       61 ccctttgaat ttacatagcg caaaaagata cctttcctca taatgtgagc taattttatg
      121 tttcgtttga tgatcgggtc ggtctcgaaa accggagtag gggcaactct accgggggtt
      181 caaatccccc tctctccgcc actttatcaa tgacttatct cccgacttcc cgtcttgctt
      241 ttcctaaaca gaacaatcgt agaatattct tgaagggtta gatcgtcact gttttctgtt
      301 cgatactgtg acattcagca cttgattcgc tatggatctg acaggaaggt tttgagcgaa
      361 aatctgcagt tattcagtcg ttttcttatc ggtcaccatt attcttttag acattgatcc
      421 tacaaagctg ccgcaaagtt ggtggtggga actgaagttg cgtagagaag gggtcaatac
      481 ccggaggcaa acatgggctg gcaaaagtgt agcggtatta ggcgcagc
//
LOCUS       DBFIFM010000553          527 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.565, whole genome shotgun sequence.
ACCESSION   DBFIFM010000553 DBFIFM010000000
VERSION     DBFIFM010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.565"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..235)
                     /gene="tomB"
                     /locus_tag="N7C74_005184"
     CDS             complement(<1..235)
                     /gene="tomB"
                     /locus_tag="N7C74_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HGU2434822.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDE"
BASE COUNT          143 a          111 c          112 g          161 t
ORIGIN      
        1 attcgtcgat ctgctcaatg agcttattgt cttcattata cttaattttg taattaagtg
       61 cgaaggtcgc aatatgctca atcagttcat tcaactggag gttgatcgcc gaggttgggt
      121 cgttaaccca gccatgattg ctttcttcaa ggtttgcaag gcagtcatga tacagagttt
      181 cacagagaaa cttaagctgt gcgatatcat gtcttttggg tgagtattca tccataacgc
      241 gtccccttct tagcggttga actaacggac acctttcggg atggaaaaaa cttactgacc
      301 tggacttgcc ctctcgtttg ttagcttaac tataagccac tctttgcagg ttttcatcgc
      361 attattacga aaaattacaa ttaggaagga agcgggacag ggctcacttt ttcatactag
      421 caccaacccg cagcaggtaa aagcagtttg ctcgaactgt tcataacatt tcaggattat
      481 cgccagatga aattggtgac actaatacca ggattgctct gaatatg
//
LOCUS       DBFIFM010000554          526 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.566, whole genome shotgun sequence.
ACCESSION   DBFIFM010000554 DBFIFM010000000
VERSION     DBFIFM010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.566"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..>526
                     /locus_tag="N7C74_005185"
     CDS             <1..>526
                     /locus_tag="N7C74_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HGU2434823.1"
                     /translation="FISPLLRRRLDDKFLRNAENQAFLVETVTAINTIKSMAVSPQMI
                     ATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVISLWMGAHLVISGEISIGQLI
                     AFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNTPVEKKSGRNILPEIQGDIE
                     FKNVRFRYSSDGNVI"
BASE COUNT          153 a           90 c          129 g          154 t
ORIGIN      
        1 gtttatatca cccttattac gtcgacgtct tgatgataag tttctcagga atgcagaaaa
       61 tcaagctttt cttgtcgaaa cggtaacagc aataaataca atcaaatcca tggcagtatc
      121 accacagatg attgctacat gggataaaca actggccggg tatgttgctt ccagtttcag
      181 ggtaaatctg gttgcaatga cagggcagca ggggatacag ctgatacaga aaagtgtaat
      241 ggtaattagt ctatggatgg gagcacatct tgtcatatcg ggagagataa gcatcgggca
      301 gttaatcgcg tttaatatgc ttgcaggtca ggtcattgct cctgttatca gactggctca
      361 tctttggcag gattttcagc aggttggtat ttctgttgag cgccttggtg atgtattaaa
      421 tacaccggta gagaaaaagt caggcagaaa tatactgccg gaaattcagg gggatatcga
      481 atttaaaaat gtcagatttc ggtattcttc tgacggtaat gttatt
//
LOCUS       DBFIFM010000555          525 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.567, whole genome shotgun sequence.
ACCESSION   DBFIFM010000555 DBFIFM010000000
VERSION     DBFIFM010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.567"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..>525)
                     /locus_tag="N7C74_005186"
     CDS             complement(<1..>525)
                     /locus_tag="N7C74_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGU2434824.1"
                     /translation="PEPIASTAFAIIHPYTAMDANFIYYYLRSPVFVCYVENCQTGVA
                     YPAINDKQFFSGITPVPPSLEQVRIANKIKELMSLCDQLEQQSLTSLDAHQQLVETLL
                     GTLTDSQTAEELAENWARISEYFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEP
                     ASELLKRIAQEKAQL"
BASE COUNT          133 a          108 c          132 g          152 t
ORIGIN      
        1 cagttgagct ttttcctgcg caattcgttt gagcagttca gaggctggtt catcattcgg
       61 atcctgcggc acaagtttgc ccattacggc cagttgcaga atggtctgtt ttaacgcatc
      121 cacgctggct tcggtggtaa atagagtgtc gaaatactcg ctaatacgcg cccagttttc
      181 agccagttcc tcggcggttt ggctgtctgt aagcgttccc aacagggttt caaccagttg
      241 ttgatgtgcg tccagactgg tcagggattg ctgttccagt tggtcgcaga gggacattaa
      301 ttctttgatt ttgtttgcga tgcgaacttg ttccaaggat ggaggaactg gagttattcc
      361 agaaaaaaat tgtttgtcat tgattgctgg ataggcaacc cctgtctgac aattttcaac
      421 ataacaaaca aataccggag aacgtagata ataataaatg aaattagcat ccatcgccgt
      481 atatggatga ataatagcga aggcagtact agcgatcggt tcagg
//
LOCUS       DBFIFM010000556          523 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.568, whole genome shotgun sequence.
ACCESSION   DBFIFM010000556 DBFIFM010000000
VERSION     DBFIFM010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.568"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..>523
                     /gene="nleH1-2"
                     /locus_tag="N7C74_005187"
     CDS             <1..>523
                     /gene="nleH1-2"
                     /locus_tag="N7C74_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-2"
                     /protein_id="HGU2434825.1"
                     /translation="LSSVRDAAVHSDSGTQVTVGNRTYRVVVTDNKFCVTRESHSGCF
                     TNLLHRLGWPKGEISRKIEAMLNTSPVSTTIERGSVHSNRPDLPPVDYAQPELPPADY
                     TQSELPRVSNNKSPVPGNVIGKGGNAVVYEDMEDTTKVLKMFTISQSHEEVTSEVRCF
                     NQYYGSGSAEKIYN"
BASE COUNT          157 a           87 c          136 g          143 t
ORIGIN      
        1 ttatcctctg taagggatgc tgctgttcac tctgatagcg ggacgcaagt aacggttggc
       61 aacagaacat atcgtgttgt ggtcactgat aataagtttt gcgttacaag agaaagtcat
      121 agtggttgtt ttactaatct gttgcacagg ttgggatggc ctaagggaga gattagcaga
      181 aaaattgagg ctatgctgaa tacatcgcca gtgagcacga ctatagaaag aggctctgtt
      241 cattcgaaca gacctgattt acctccagtg gattatgcgc agccggagtt acctccagcg
      301 gattatactc aatcagagtt gccgagggtt agcaacaata aatcacccgt gccaggtaat
      361 gttattggta aaggtggtaa tgctgtcgtg tatgaagata tggaagatac aacaaaagtg
      421 ttgaagatgt ttactatatc tcaaagccat gaagaggtga caagcgaagt tcgttgtttc
      481 aatcagtatt atggttccgg gagtgcagag aaaatatata atg
//
LOCUS       DBFIFM010000557          514 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.569, whole genome shotgun sequence.
ACCESSION   DBFIFM010000557 DBFIFM010000000
VERSION     DBFIFM010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.569"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..482
                     /locus_tag="N7C74_005188"
     CDS             <1..482
                     /locus_tag="N7C74_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGU2434826.1"
                     /translation="TTLRDKAMVNYAFDYLSSPGSLPFTTAATELSAIHGHSTSQYRL
                     GEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLGVKPDTRKAILWY
                     KEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQKWLKLAAKQHYKNAERLLAGLPAH"
BASE COUNT          156 a          117 c          117 g          124 t
ORIGIN      
        1 aaacgacgtt gcgtgataaa gccatggtga attatgcctt tgattattta agctcaccgg
       61 gcagtcttcc attcaccacg gcagccacgg agctttccgc gattcatggt cactcaacgt
      121 cgcaatatcg ccttggagaa ttttatcttc atggtagcga cggtaaacca ctggattata
      181 cacaggcgag atactggtat gagcaatcag cggaacagga aaatccacgc gcgcaaagta
      241 aactggggtg gatctacctc aaaggtctgg gggtcaaacc cgacacccgt aaagcaattc
      301 tctggtataa ggaagcagct gaacaagggt atgctcatgc tcaatatact ttaggtttga
      361 tctacagaaa tggctcaggt attaatgtta accattatga atctcaaaaa tggttaaaac
      421 tggccgccaa acaacattac aaaaatgcgg aaagattact tgccgggctt cccgcacatt
      481 aacgtcgact gtcacccgaa cagatggagt attt
//
LOCUS       DBFIFM010000558          513 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.denovo.570, whole genome shotgun sequence.
ACCESSION   DBFIFM010000558 DBFIFM010000000
VERSION     DBFIFM010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.denovo.570"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            <1..>513
                     /gene="espK"
                     /locus_tag="N7C74_005189"
     CDS             <1..>513
                     /gene="espK"
                     /locus_tag="N7C74_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HGU2434827.1"
                     /translation="ATDSIKNVPNGLSSLSINSYNPENQARIDNLISPSLKTLSLTGC
                     SNIILPEKLPESVTSVTIHAEQKTTWNIGVEGMPDGLDLDLQNVLLSPDVVKAKNITF
                     QGNALDVALHFREGDIVYGLSSPREKLVNSIKLVNDFSKKDIITQNTLTNAVWDPRTP
                     RKYKQDPLIK"
BASE COUNT          173 a          118 c          104 g          118 t
ORIGIN      
        1 cgccacagac agcataaaaa atgttccaaa cgggttatca tctctcagca tcaatagcta
       61 taacccggag aatcaggcca gaattgacaa cctgatatca ccgtcactga agacgctatc
      121 gctgactgga tgtagcaata ttatactgcc ggagaaactt ccggagagtg tgacatcggt
      181 aaccattcat gcggagcaga aaaccacgtg gaacatcggt gttgaaggga tgcctgatgg
      241 gctggatctt gatttacaaa atgtactact ctctccagat gtagttaaag caaaaaacat
      301 cacctttcag ggcaacgctc tggatgtggc cttacacttt cgcgagggag acattgtcta
      361 tggactatct tcacccagag aaaaacttgt caacagcatt aaactagtta acgacttttc
      421 caaaaaagat attataactc agaatacgtt aacaaacgca gtatgggacc ccagaacacc
      481 tcgcaaatat aagcaagatc cacttatcaa aag
//
LOCUS       DBFIFM010000559         4524 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.guided.280, whole genome shotgun sequence.
ACCESSION   DBFIFM010000559 DBFIFM010000000
VERSION     DBFIFM010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.guided.280"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            87..1220
                     /locus_tag="N7C74_005190"
     CDS             87..1220
                     /locus_tag="N7C74_005190"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HGU2434828.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            1308..1841
                     /gene="blc"
                     /locus_tag="N7C74_005191"
     CDS             1308..1841
                     /gene="blc"
                     /locus_tag="N7C74_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HGU2434829.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1838..2155)
                     /gene="sugE"
                     /locus_tag="N7C74_005192"
     CDS             complement(1838..2155)
                     /gene="sugE"
                     /locus_tag="N7C74_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HGU2434830.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2331..2477)
                     /gene="ecnB"
                     /locus_tag="N7C74_005193"
     CDS             complement(2331..2477)
                     /gene="ecnB"
                     /locus_tag="N7C74_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HGU2434831.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2588..2713)
                     /gene="ecnA"
                     /locus_tag="N7C74_005194"
     CDS             complement(2588..2713)
                     /gene="ecnA"
                     /locus_tag="N7C74_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HGU2434832.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(2765..3331)
                     /gene="efp"
                     /locus_tag="N7C74_005195"
     CDS             complement(2765..3331)
                     /gene="efp"
                     /locus_tag="N7C74_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGU2434833.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3373..4401
                     /gene="epmB"
                     /locus_tag="N7C74_005196"
     CDS             3373..4401
                     /gene="epmB"
                     /locus_tag="N7C74_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HGU2434834.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRAGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         1155 a         1154 c         1126 g         1089 t
ORIGIN      
        1 ctgattggtg tcgttacaat ctaacgcatc gccaatgtaa atccggcccg cctatggcgg
       61 gccgttttgt atggaaacca gaccctatgt tcaaaacgac gctctgcgcc ttattaatta
      121 ccgcctcttg ctccacattt gctgcccctc aacaaatcaa cgatattgtg catcgcacaa
      181 ttaccccgct tatagagcaa caaaagatcc ccggtatggc ggtggcggta atttatcagg
      241 gtaaacctta ttactttacc tgggggtatg cggacatcgc caaaaagcag cccgtcacac
      301 agcaaacgtt gtttgagtta ggttcggtca gcaaaacatt tacgggcgtg cttggtggcg
      361 acgctattgc tcgaggggaa atcaagttaa gcgatcccgc aacaaaatac tggcctgaac
      421 ttaccgctaa acagtggaat gggatcacac tattacatct cgcaacctac actgctggcg
      481 gcctgccatt gcaggtgccg gatgaagtga aatcctcaag cgacttgctg cgcttctatc
      541 aaaactggca gcctgcatgg gctccaggaa cacaacgtct gtatgccaac tccagtatcg
      601 gtttgttcgg cgcactggct gtgaagccgt ctggtttgag ttttgagcag gcgatgcaaa
      661 ctcgtgtctt ccagccactc aaactcaacc atacgtggat taatgtaccg cccgcagaag
      721 aaaagaatta cgcctgggga tatcgcgaag gcaaggcagt tcatgtttcg ccaggggcgt
      781 tagatgctga aacttatggt gtgaagtcga ccattgaaga tatggcctgc tgggtacgaa
      841 gcaatatgaa tccccgtgat atcaacgaca aaacacttca gcaagggata caactggcac
      901 aatctcgcta ctggcaaacc ggcgatatgt atcagggtct gggctgggaa atgctggact
      961 ggccggtaaa tcctgacatc atcgttaacg gcagtgacaa taaaattgca ctggcagcac
     1021 gccccgtaaa agcgattacg cctccaactc ctgcagtacg agcatcatgg gtgcataaaa
     1081 cgggggcgac cggcggattt ggtagctatg tcgcgtttat tccagaaaaa gagctgggta
     1141 tcgtgatgct ggctaacaaa aactatccca atccagcgag agtcgccgcc gcctggcaga
     1201 ttctcaacgc tctacagtaa aatttcatcg ggtccgaatt ttcggacctt ttctgcgctt
     1261 ttccttgctg tcatctacac ttagaaaaaa ccagtaagga aacatttatg cgcctgctcc
     1321 ctctcgttgc cgcagcgaca gctgcatttc tggtcgttgc ctgcagttct cctacgccgc
     1381 cgcgtggcgt gaccgtagtg aataatttcg acgctaaacg ctatctcggt acctggtatg
     1441 agattgcccg ttttgatcac cgctttgaac gtggactgga aaaagtcacc gcaacataca
     1501 gcctgcgtga tgacggcggc ctgaatgtca ttaataaagg ctataaccct gacagaggaa
     1561 tgtggcagca gagtgaaggg aaagcgtact ttaccggcgc accaactcgc gctgcgctga
     1621 aagtgtcatt ctttggtcct ttctatggcg gttataacgt tattgcactc gatcgggaat
     1681 accgccatgc gctggtttgc ggaccggacc gcgactacct gtggatactc tcccgcacgc
     1741 caaccatttc tgacgaagtg aaacaggaga tgctggcagt cgcgacccgg gaagggtttg
     1801 atgtcagtaa gtttatttgg gtacagcagc ccggtagtta gtgagtgctg agtttcagac
     1861 caataatccc caatacgatt aacgccagac tcgccagacg catcgggtta gcggactcac
     1921 cgagcagcac aatgccggtg atggccgcgc cgactgcgcc aatacccgtc cacacggcat
     1981 aagccgtccc tactggtaac gatttcatcg cccaggcaag tagcgccata ctgacaatca
     2041 tcgccgtcac ggtaataaca ctcggcgtca aacgactaaa gccgtgggta tatttcaggc
     2101 ctacggccca taccacttcc agcagaccag caataactaa gataatccag gacatatcag
     2161 gctccggaga attggggccg tccccggtga aaagacgcgt ttgcaggtcg tcctgcaaag
     2221 ctgatggttg aacggatatt ttgccaggtg gcgggggaat ttcaactatt acctttgtga
     2281 ctatcgcaga aaaaacaaac ggcacgacac agctgtcgta ccgttattgc ttattgctgc
     2341 gctttcgttg ctgcaccaga aatcgcgtta ccgccatcag aaatgtcttc accaacgcca
     2401 cgcgtggtgt tgcaggcagt taatactgtt gaaagcacca gaacagaaaa gatcgctgca
     2461 attgtcttct tcaccataac gtcttccttt taggtaatgt tatttatgtt tgcctatagc
     2521 aaattaataa tagacaacat cgccaaaaat gatggaaatc tgatgatttt tcggaagaga
     2581 agaaaaatta gctggcagcg cgggagatgg agttgccgag atgtttgatg tcttcgccga
     2641 aaccgcgagc ggtgttacag cccgtgagca gcgtgctggc aagcaaaaca agaacgataa
     2701 ggcgtttcat catctctgac ctgaataagt gatggtgcag cctgcaggcc gcaccacaac
     2761 cgcattactt cacgcgagag acgtattcac cagagcgggt atccactttg atgacttcgc
     2821 cgatttgtac gaacagcgga actttaacca cagcgccagt agacagggta gccggtttgc
     2881 cgccagtacc tgcggtatca cctttcaggc ccggatcggt atcaacgatt tccagttcaa
     2941 cgaagttcgg cggagtaacg gagatcggct gaccattcca cagagttacg atacactctg
     3001 cctgatccag cagccattta gcgttgtcgc caattgcttt tgcgtcagca gacagctgct
     3061 cgaaagtttc gttgttcatg aagtgccaga actcaccgtc gttgtacagg taagtcaggt
     3121 tcatatcgac aacatcagcg ccttcagcgg aatcagtaga tttgaaggtt ttttctacgc
     3181 gagtaccggt cagcagacga cgcagtttaa cgcgagcaaa tgcctggcct ttacccggtt
     3241 ttacgaattc actcgcttca accgcgtaag gttcgccgtc taacatgatt ttaagaccag
     3301 cacgaaaatc gttgctatag tacgttgcca taaggccctc tgaaatttgt taattggtag
     3361 ctaagccaca aaatggcgca tattgtaacc ctaaataccc catccagaga agattggtta
     3421 acgcaacttg ccgatgttgt gaccgatcct gatgaacttc tgcgtctttt gaatatagac
     3481 gcggacgaaa aactgttggc cggacgcaac gccaaaaagc tgtttgccct gcgtgtgccc
     3541 cgctcattta tcgatcgcat ggagaaaggc aatccggacg atccgctttt gcgtcaggta
     3601 cttacctcgc aagatgagtt tgtcgtcgcg cccggattct ccaccgaccc gctggaagaa
     3661 cagcacagcg tagtgcctgg tttgttgcat aaataccaca accgggcgct tttgctggtc
     3721 aaaggcggct gcgcggtaaa ttgccgctat tgcttccgtc gccacttccc ctatgccgaa
     3781 aatcagggca acaagcgtaa ctggcaaact gcacttgagt atgttgctgc gcatccggaa
     3841 ctggacgaga tgattttctc cggcggcgat ccgctgatgg cgaaagatca cgagctggac
     3901 tggttgctca cacaactgga agccatcccg catatcaaac gtctgcggat tcacagtcgt
     3961 ctgccgattg tgatcccggc acgcatcaca gatgcgctgg ttgaacgctt ttcacattct
     4021 acgctgcaaa tcttgctggt gaatcacatc aaccatgcca atgagataga tgaaacattc
     4081 cgtcaggcga tggctaagtt gcgccgggcg ggcgttactt tgctgaacca gagcgttctg
     4141 ttacgtggtg tgaacgataa cgcacaaacg ctggcaaacc tgagtaatgc gttgttcgat
     4201 gcaggcgtga tgccgtatta cctgcatgtg ctcgataaag tacagggcgc ggcgcatttt
     4261 atggtgagtg atgacgaagc acggcagatt atgcgtgagt tgctgacact ggtgtcggga
     4321 tatctggtgc cgaaactggc gcgagaaatt ggcggcgaac ccagcaaaac gccgctggat
     4381 ctccagctac gccagcagta actgattaaa cagggaagtt taatctttgc ccgttacata
     4441 acctttaatt attcatatac attgctgttg ttactcttta tataaccaac accaataatt
     4501 aacctttctt ttctgtttat atag
//
LOCUS       DBFIFM010000560         3206 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.guided.320, whole genome shotgun sequence.
ACCESSION   DBFIFM010000560 DBFIFM010000000
VERSION     DBFIFM010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.guided.320"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..286)
                     /locus_tag="N7C74_005197"
     CDS             complement(<1..286)
                     /locus_tag="N7C74_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="catalyzes the hydrolysis of Xaa-His dipeptides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HGU2434835.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDP"
     gene            547..1005
                     /gene="gpt"
                     /locus_tag="N7C74_005198"
     CDS             547..1005
                     /gene="gpt"
                     /locus_tag="N7C74_005198"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGU2434836.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1097..2341
                     /gene="frsA"
                     /locus_tag="N7C74_005199"
     CDS             1097..2341
                     /gene="frsA"
                     /locus_tag="N7C74_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HGU2434837.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSCLLHQRVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            2399..2800
                     /gene="crl"
                     /locus_tag="N7C74_005200"
     CDS             2399..2800
                     /gene="crl"
                     /locus_tag="N7C74_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HGU2434838.1"
                     /translation="MTLPSGHPKSRLIKKFTVLGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(2839..>3206)
                     /locus_tag="N7C74_005201"
     CDS             complement(2839..>3206)
                     /locus_tag="N7C74_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HGU2434839.1"
                     /translation="WATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQY
                     QFDFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSD
                     NKLNINNDDIVAVGMTYQF"
BASE COUNT          800 a          756 c          821 g          829 t
ORIGIN      
        1 taggatcttt cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc atatcgaggt
       61 gggcctgcaa gacgaccggt ttacgatttt ccatacctgc ggtagcaggt ttacgaatca
      121 ggatattacc tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa
      181 tgtattcagc gagttgctct tcatgatagg acgggtgagg aatagaacag attttggcaa
      241 aaatatccca cagcggctgt ggagataatt gagacagttc agacacgtta agtctccttg
      301 tcgatcaccc gcaaaacagt attgcaggtc acagggttag cagaaaatgt tgtcaacaca
      361 agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt
      421 gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat
      481 ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca
      541 cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa
      601 ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc
      661 ggtctggtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt
      721 tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa
      781 ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt
      841 gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tctttgcaaa accggctggt
      901 cgtccgctgg ttgatgatta tgttgttgat atcccgcaag atacctggat cgaacagccg
      961 tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg
     1021 gcactgccgg gcgttgttct ttttaacttc aggcgagtta caatagtttc cagtaagtat
     1081 tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt
     1141 taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt
     1201 gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat
     1261 gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac gtattgtgat
     1321 gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggcgg
     1381 caactggatt tatgagtggg ccacccaggc gatggtgtgg caacaaaaag cctgtgcgga
     1441 agaagatccg caactcagtg gtcgtcactg gctgcatgcg gctacgttgt acaacattgc
     1501 cgcctatcct catctgaaag gagatgacct ggccgagcaa gcgcaggctt tgtcaaaccg
     1561 cgcctatgaa gaggccgctc agcgtctacc gggcacgatg cgacagatgg agtttaccgt
     1621 acccggcggt gcgcccatca ccggcttttt gcatatgccg aaaggcgatg gcccgttccc
     1681 gacggtatta atgtgtggtg gtctggatgc gatgcagacg gactattaca gcctgtatga
     1741 acgttatttt gcgccgcgcg gcattgcgat gctgactatt gatatgccgt cggtgggctt
     1801 ttcttcaaaa tggaagctca cccaggactc ctgcctgttg catcagcgcg tcttaaaggc
     1861 tctgcctaac gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg
     1921 cgctaacgtt gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag cggttgcctg
     1981 tcttggtccg gtagttcata ccctgttgag tgattttaag tgccagcaac aggtgccgga
     2041 aatgtatctt gacgttctgg cgagtcgttt ggggatgcat gatgcttccg acgaagcgtt
     2101 gcgcgtggag ctgaatcgct attcattaaa agtgcaagga ttgctgggac gtcgatgccc
     2161 aacgccaatg ttatcaggct actggaagaa cgatccgttc agcccggaag aggactcacg
     2221 cttaatcacc tcatcatctg ctgacggtaa attattagag atcccattta acccggtgta
     2281 tcggaatttt gacaaaggtc ttcaggaaat caccgactgg atcgaaaaac gcttgtgtta
     2341 aaaatttgct aaattttgcc aatttggtaa aacagttgca tcacaacagg agatagcaat
     2401 gacgttaccg agtggacacc cgaagagcag attgatcaaa aaatttaccg tactaggccc
     2461 gtatattcgt gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc tggctgtatg
     2521 cgtcaacgtg aaaccggcac cggaagtgcg tgagttctgg ggctggtgga tggagcttga
     2581 agcgcaggaa tcccgtttta cctacagtta ccagtttggt ctgttcgata aagcaggcga
     2641 ctggaagagt gttccggtaa aagatactga agtggttgaa cgactggagc acaccctgcg
     2701 tgagtttcac gagaagctgc gtgaactgct gacgacgctg aatctgaagt tggaaccggc
     2761 ggatgatttt cgtgacgagc cggtgaagtt aacggcgtga gtgaaatgtg ccggatgcat
     2821 cacatccggc aataatcatt aaaactgata ggtcatgcca accgcgacaa tatcatcatt
     2881 attaatattc aatttgttat cgctatccag ttggttgatt ttataatcaa caaacgctga
     2941 catatttttg ttgaaataat atgtagcccc gacatcgata taattgacca gatcttcatc
     3001 accgatacct tcaatatctt tccctttcga taagacataa cccagcgatg gacgcagacc
     3061 aaagtcaaac tggtattgag cgaccgcttc aaagttctgt gtcttattgg caaagccgcc
     3121 agttattggc gtcattttgc gtgtttcaga ataaaaagtt gccagataaa tattattggc
     3181 atcgtatttc agacctgtag cccaag
//
LOCUS       DBFIFM010000561         1373 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.guided.414, whole genome shotgun sequence.
ACCESSION   DBFIFM010000561 DBFIFM010000000
VERSION     DBFIFM010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.guided.414"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            45..1004
                     /gene="stxA2"
                     /locus_tag="N7C74_005202"
     CDS             45..1004
                     /gene="stxA2"
                     /locus_tag="N7C74_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A, a/c/d subtype"
                     /protein_id="HGU2434840.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDIYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1016..1285
                     /locus_tag="N7C74_005203"
     CDS             1016..1285
                     /locus_tag="N7C74_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGU2434841.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          380 a          270 c          337 g          386 t
ORIGIN      
        1 ccgggtctgg tgctgattac ttcagccaaa aggaacacct gtatatgaag tgtatattat
       61 ttaaatgggt actgtgcctg ttactgggct tttcttcggt atcctattcc cgggaattta
      121 cgatagactt ttcgactcaa caaagttatg tatcttcgtt aaatagtata cggacagaga
      181 tatcgacccc tcttgaacat atatctcagg ggaccacatc ggtgtctgtt attaaccaca
      241 ccccaccggg cagttatttt gctgtggata tacgagggct tgatatctat caggcgcgtt
      301 ttgaccatct tcgtctgatt attgagcaaa ataatttata tgtggccggg ttcgttaata
      361 cggcaacaaa tactttctac cgtttttcag attttacaca tatatcagtg cccggtgtga
      421 caacggtttc catgacaacg gacagcagtt ataccactct gcaacgtgtc gcagcgctgg
      481 aacgttccgg aatgcaaatc agtcgtcact cactggtttc atcatatctg gcgttaatgg
      541 agttcagtgg taatacaatg accagagatg catccagagc agttctgcgt tttgtcactg
      601 tcacagcaga agccttacgc ttcaggcaga tacagagaga atttcgtcag gcactgtctg
      661 aaactgctcc tgtgtatacg atgacgccgg gagacgtgga cctcactctg aactgggggc
      721 gaatcagcaa tgtgcttccg gagtatcggg gagaggatgg tgtcagagtg gggagaatat
      781 cctttaataa tatatcagcg atactgggga ctgtggccgt tatactgaat tgccatcatc
      841 agggggcgcg ttctgttcgc gccgtgaatg aagagagtca accagaatgt cagataactg
      901 gcgacaggcc tgttataaaa ataaacaata cattatggga aagtaataca gctgcagcgt
      961 ttctgaacag aaagtcacag tttttatata caacgggtaa ataaaggagt taagcatgaa
     1021 gaagatgttt atggcggttt tatttgcatt agcttctgtt aatgcaatgg cggcggattg
     1081 tgctaaaggt aaaattgagt tttccaagta taatgaggat gacacattta cagtgaaggt
     1141 tgacgggaaa gaatactgga ccagtcgctg gaatctgcaa ccgttactgc aaagtgctca
     1201 gttgacagga atgactgtca caatcaaatc cagtacctgt gaatcaggct ccggatttgc
     1261 tgaagtgcag tttaataatg actgaggcat aacctgattc gtggtatgtg ggtaacaagt
     1321 gtaatctgtg tcacaattca gtcagttgac agttgcctgt cagactgagc att
//
LOCUS       DBFIFM010000562         1373 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.guided.415, whole genome shotgun sequence.
ACCESSION   DBFIFM010000562 DBFIFM010000000
VERSION     DBFIFM010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.guided.415"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            45..1004
                     /gene="stxA2"
                     /locus_tag="N7C74_005204"
     CDS             45..1004
                     /gene="stxA2"
                     /locus_tag="N7C74_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A, a/c/d subtype"
                     /protein_id="HGU2434842.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDIYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1016..1285
                     /locus_tag="N7C74_005205"
     CDS             1016..1285
                     /locus_tag="N7C74_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGU2434843.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          379 a          270 c          337 g          387 t
ORIGIN      
        1 ccgggtctgg tgctgattac ttcagccaaa aggaacacct gtatatgaag tgtatattat
       61 ttaaatgggt actgtgcctg ttactgggct tttcttcggt atcctattcc cgggaattta
      121 cgatagactt ttcgactcaa caaagttatg tatcttcgtt aaatagtata cggacagaga
      181 tatcgacccc tcttgaacat atatctcagg ggaccacatc ggtgtctgtt attaaccaca
      241 ccccaccggg cagttatttt gctgtggata tacgagggct tgatatctat caggcgcgtt
      301 ttgaccatct tcgtctgatt attgagcaaa ataatttata tgtggccggg ttcgttaata
      361 cggcaacaaa tactttctac cgtttttcag attttacaca tatatcagtg cccggtgtga
      421 caacggtttc catgacaacg gacagcagtt ataccactct gcaacgtgtc gcagcgctgg
      481 aacgttccgg aatgcaaatc agtcgtcact cactggtttc atcatatctg gcgttaatgg
      541 agttcagtgg taatacaatg accagagatg catccagagc agttctgcgt tttgtcactg
      601 tcacagcaga agccttacgc ttcaggcaga tacagagaga atttcgtcag gcactgtctg
      661 aaactgctcc tgtgtatacg atgacgccgg gagacgtgga cctcactctg aactgggggc
      721 gaatcagcaa tgtgcttccg gagtatcggg gagaggatgg tgtcagagtg gggagaatat
      781 cctttaataa tatatcggcg atactgggca ctgtggccgt tatactgaat tgtcatcatc
      841 agggggcgcg ttctgttcgc gccgtgaatg aagagagtca accagaatgt cagataactg
      901 gcgacaggcc tgttataaaa ataaacaata cattatggga aagtaataca gctgcagcgt
      961 ttctgaacag aaagtcacag tttttatata caacgggtaa ataaaggagt taagcatgaa
     1021 gaagatgttt atggcggttt tatttgcatt agcttctgtt aatgcaatgg cggcggattg
     1081 tgctaaaggt aaaattgagt tttccaagta taatgaggat gacacattta cagtgaaggt
     1141 tgacgggaaa gaatactgga ccagtcgctg gaatctgcaa ccgttactgc aaagtgctca
     1201 gttgacagga atgactgtca caatcaaatc cagtacctgt gaatcaggct ccggatttgc
     1261 tgaagtgcag tttaataatg actgaggcat aacctgattc gtggtatgtg ggtaacaagt
     1321 gtaatctgtg tcacaattca gtcagttgac agttgcctgt cagactgagc att
//
LOCUS       DBFIFM010000563         1373 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.guided.416, whole genome shotgun sequence.
ACCESSION   DBFIFM010000563 DBFIFM010000000
VERSION     DBFIFM010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.guided.416"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            45..1004
                     /locus_tag="N7C74_005206"
     CDS             45..1004
                     /locus_tag="N7C74_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU2434844.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1016..1285
                     /locus_tag="N7C74_005207"
     CDS             1016..1285
                     /locus_tag="N7C74_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGU2434845.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          379 a          270 c          338 g          386 t
ORIGIN      
        1 ccgggtctgg tgctgattac ttcagccaaa aggaacacct gtatatgaag tgtatattat
       61 ttaaatgggt actgtgcctg ttactgggct tttcttcggt atcctattcc cgggaattta
      121 cgatagactt ttcgactcaa caaagttatg tatcttcgtt aaatagtata cggacagaga
      181 tatcgacccc tcttgaacat atatctcagg ggaccacatc ggtgtctgtt attaaccaca
      241 ccccaccggg cagttatttt gctgtggata tacgagggct tgatgtctat caggcgcgtt
      301 ttgaccatct tcgtctgatt attgagcaaa ataatttata tgtggccggg ttcgttaata
      361 cggcaacaaa tactttctac cgtttttcag attttacaca tatatcagtg cccggtgtga
      421 caacggtttc catgacaacg gacagcagtt ataccactct gcaacgtgtc gcagcgctgg
      481 aacgttccgg aatgcaaatc agtcgtcact cactggtttc atcatatctg gcgttaatgg
      541 agttcagtgg taatacaatg accagagatg catccagagc agttctgcgt tttgtcactg
      601 tcacagcaga agccttacgc ttcaggcaga tacagagaga atttcgtcag gcactgtctg
      661 aaactgctcc tgtgtatacg atgacgccgg gagacgtgga cctcactctg aactgggggc
      721 gaatcagcaa tgtgcttccg gagtatcggg gagaggatgg tgtcagagtg gggagaatat
      781 cctttaataa tatatcagcg atactgggga ctgtggccgt tatactgaat tgccatcatc
      841 agggggcgcg ttctgttcgc gccgtgaatg aagagagtca accagaatgt cagataactg
      901 gcgacaggcc tgttataaaa ataaacaata cattatggga aagtaataca gctgcagcgt
      961 ttctgaacag aaagtcacag tttttatata caacgggtaa ataaaggagt taagcatgaa
     1021 gaagatgttt atggcggttt tatttgcatt agcttctgtt aatgcaatgg cggcggattg
     1081 tgctaaaggt aaaattgagt tttccaagta taatgaggat gacacattta cagtgaaggt
     1141 tgacgggaaa gaatactgga ccagtcgctg gaatctgcaa ccgttactgc aaagtgctca
     1201 gttgacagga atgactgtca caatcaaatc cagtacctgt gaatcaggct ccggatttgc
     1261 tgaagtgcag tttaataatg actgaggcat aacctgattc gtggtatgtg ggtaacaagt
     1321 gtaatctgtg tcacaattca gtcagttgac agttgcctgt cagactgagc att
//
LOCUS       DBFIFM010000564         1373 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.guided.417, whole genome shotgun sequence.
ACCESSION   DBFIFM010000564 DBFIFM010000000
VERSION     DBFIFM010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.guided.417"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            45..1004
                     /locus_tag="N7C74_005208"
     CDS             45..1004
                     /locus_tag="N7C74_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU2434846.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1016..1285
                     /locus_tag="N7C74_005209"
     CDS             1016..1285
                     /locus_tag="N7C74_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGU2434847.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          378 a          270 c          338 g          387 t
ORIGIN      
        1 ccgggtctgg tgctgattac ttcagccaaa aggaacacct gtatatgaag tgtatattat
       61 ttaaatgggt actgtgcctg ttactgggct tttcttcggt atcctattcc cgggaattta
      121 cgatagactt ttcgactcaa caaagttatg tatcttcgtt aaatagtata cggacagaga
      181 tatcgacccc tcttgaacat atatctcagg ggaccacatc ggtgtctgtt attaaccaca
      241 ccccaccggg cagttatttt gctgtggata tacgagggct tgatgtctat caggcgcgtt
      301 ttgaccatct tcgtctgatt attgagcaaa ataatttata tgtggccggg ttcgttaata
      361 cggcaacaaa tactttctac cgtttttcag attttacaca tatatcagtg cccggtgtga
      421 caacggtttc catgacaacg gacagcagtt ataccactct gcaacgtgtc gcagcgctgg
      481 aacgttccgg aatgcaaatc agtcgtcact cactggtttc atcatatctg gcgttaatgg
      541 agttcagtgg taatacaatg accagagatg catccagagc agttctgcgt tttgtcactg
      601 tcacagcaga agccttacgc ttcaggcaga tacagagaga atttcgtcag gcactgtctg
      661 aaactgctcc tgtgtatacg atgacgccgg gagacgtgga cctcactctg aactgggggc
      721 gaatcagcaa tgtgcttccg gagtatcggg gagaggatgg tgtcagagtg gggagaatat
      781 cctttaataa tatatcggcg atactgggca ctgtggccgt tatactgaat tgtcatcatc
      841 agggggcgcg ttctgttcgc gccgtgaatg aagagagtca accagaatgt cagataactg
      901 gcgacaggcc tgttataaaa ataaacaata cattatggga aagtaataca gctgcagcgt
      961 ttctgaacag aaagtcacag tttttatata caacgggtaa ataaaggagt taagcatgaa
     1021 gaagatgttt atggcggttt tatttgcatt agcttctgtt aatgcaatgg cggcggattg
     1081 tgctaaaggt aaaattgagt tttccaagta taatgaggat gacacattta cagtgaaggt
     1141 tgacgggaaa gaatactgga ccagtcgctg gaatctgcaa ccgttactgc aaagtgctca
     1201 gttgacagga atgactgtca caatcaaatc cagtacctgt gaatcaggct ccggatttgc
     1261 tgaagtgcag tttaataatg actgaggcat aacctgattc gtggtatgtg ggtaacaagt
     1321 gtaatctgtg tcacaattca gtcagttgac agttgcctgt cagactgagc att
//
LOCUS       DBFIFM010000565         1373 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.guided.418, whole genome shotgun sequence.
ACCESSION   DBFIFM010000565 DBFIFM010000000
VERSION     DBFIFM010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.guided.418"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            45..1004
                     /gene="stxA2"
                     /locus_tag="N7C74_005210"
     CDS             45..1004
                     /gene="stxA2"
                     /locus_tag="N7C74_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A, a/c/d subtype"
                     /protein_id="HGU2434848.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDIYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1016..1285
                     /locus_tag="N7C74_005211"
     CDS             1016..1285
                     /locus_tag="N7C74_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGU2434849.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          379 a          271 c          337 g          386 t
ORIGIN      
        1 ccgggtctgg tgctgattac ttcagccaaa aggaacacct gtatatgaag tgtatattat
       61 ttaaatgggt actgtgcctg ttactgggtt tttcttcggt atcctattcc cgggagttta
      121 cgatagactt ttcgacccaa caaagttatg tctcttcgtt aaatagtata cgaacagaga
      181 tatcgacccc tcttgaacat atatctcagg ggaccacatc ggtgtctgtt attaaccaca
      241 ccccaccggg cagttatttt gctgtggata tacgagggct tgatatctat caggcgcgtt
      301 ttgaccatct tcgtctgatt attgagcaaa ataatttata tgtggccggg ttcgttaata
      361 cggcaacaaa tactttctac cgtttttcag attttacaca tatatcagtg cccggtgtga
      421 caacggtttc catgacaacg gacagcagtt ataccactct gcaacgtgtc gcagcgctgg
      481 aacgttccgg aatgcaaatc agtcgtcact cactggtttc atcatatctg gcgttaatgg
      541 agttcagtgg taatacaatg accagagatg catccagagc agttctgcgt tttgtcactg
      601 tcacagcaga agccttacgc ttcaggcaga tacagagaga atttcgtcag gcactgtctg
      661 aaactgctcc tgtgtatacg atgacgccgg gagacgtgga cctcactctg aactgggggc
      721 gaatcagcaa tgtgcttccg gagtatcggg gagaggatgg tgtcagagtg gggagaatat
      781 cctttaataa tatatcagcg atactgggga ctgtggccgt tatactgaat tgccatcatc
      841 agggggcgcg ttctgttcgc gccgtgaatg aagagagtca accagaatgt cagataactg
      901 gcgacaggcc tgttataaaa ataaacaata cattatggga aagtaataca gctgcagcgt
      961 ttctgaacag aaagtcacag tttttatata caacgggtaa ataaaggagt taagcatgaa
     1021 gaagatgttt atggcggttt tatttgcatt agcttctgtt aatgcaatgg cggcggattg
     1081 tgctaaaggt aaaattgagt tttccaagta taatgaggat gacacattta cagtgaaggt
     1141 tgacgggaaa gaatactgga ccagtcgctg gaatctgcaa ccgttactgc aaagtgctca
     1201 gttgacagga atgactgtca caatcaaatc cagtacctgt gaatcaggct ccggatttgc
     1261 tgaagtgcag tttaataatg actgaggcat aacctgattc gtggtatgtg ggtaacaagt
     1321 gtaatctgtg tcacaattca gtcagttgac agttgcctgt cagactgagc att
//
LOCUS       DBFIFM010000566         1373 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.guided.419, whole genome shotgun sequence.
ACCESSION   DBFIFM010000566 DBFIFM010000000
VERSION     DBFIFM010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.guided.419"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            45..1004
                     /gene="stxA2"
                     /locus_tag="N7C74_005212"
     CDS             45..1004
                     /gene="stxA2"
                     /locus_tag="N7C74_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A, a/c/d subtype"
                     /protein_id="HGU2434850.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDIYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1016..1285
                     /locus_tag="N7C74_005213"
     CDS             1016..1285
                     /locus_tag="N7C74_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGU2434851.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          378 a          271 c          337 g          387 t
ORIGIN      
        1 ccgggtctgg tgctgattac ttcagccaaa aggaacacct gtatatgaag tgtatattat
       61 ttaaatgggt actgtgcctg ttactgggtt tttcttcggt atcctattcc cgggagttta
      121 cgatagactt ttcgacccaa caaagttatg tctcttcgtt aaatagtata cgaacagaga
      181 tatcgacccc tcttgaacat atatctcagg ggaccacatc ggtgtctgtt attaaccaca
      241 ccccaccggg cagttatttt gctgtggata tacgagggct tgatatctat caggcgcgtt
      301 ttgaccatct tcgtctgatt attgagcaaa ataatttata tgtggccggg ttcgttaata
      361 cggcaacaaa tactttctac cgtttttcag attttacaca tatatcagtg cccggtgtga
      421 caacggtttc catgacaacg gacagcagtt ataccactct gcaacgtgtc gcagcgctgg
      481 aacgttccgg aatgcaaatc agtcgtcact cactggtttc atcatatctg gcgttaatgg
      541 agttcagtgg taatacaatg accagagatg catccagagc agttctgcgt tttgtcactg
      601 tcacagcaga agccttacgc ttcaggcaga tacagagaga atttcgtcag gcactgtctg
      661 aaactgctcc tgtgtatacg atgacgccgg gagacgtgga cctcactctg aactgggggc
      721 gaatcagcaa tgtgcttccg gagtatcggg gagaggatgg tgtcagagtg gggagaatat
      781 cctttaataa tatatcggcg atactgggca ctgtggccgt tatactgaat tgtcatcatc
      841 agggggcgcg ttctgttcgc gccgtgaatg aagagagtca accagaatgt cagataactg
      901 gcgacaggcc tgttataaaa ataaacaata cattatggga aagtaataca gctgcagcgt
      961 ttctgaacag aaagtcacag tttttatata caacgggtaa ataaaggagt taagcatgaa
     1021 gaagatgttt atggcggttt tatttgcatt agcttctgtt aatgcaatgg cggcggattg
     1081 tgctaaaggt aaaattgagt tttccaagta taatgaggat gacacattta cagtgaaggt
     1141 tgacgggaaa gaatactgga ccagtcgctg gaatctgcaa ccgttactgc aaagtgctca
     1201 gttgacagga atgactgtca caatcaaatc cagtacctgt gaatcaggct ccggatttgc
     1261 tgaagtgcag tttaataatg actgaggcat aacctgattc gtggtatgtg ggtaacaagt
     1321 gtaatctgtg tcacaattca gtcagttgac agttgcctgt cagactgagc att
//
LOCUS       DBFIFM010000567         1373 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.guided.420, whole genome shotgun sequence.
ACCESSION   DBFIFM010000567 DBFIFM010000000
VERSION     DBFIFM010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.guided.420"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            45..1004
                     /locus_tag="N7C74_005214"
     CDS             45..1004
                     /locus_tag="N7C74_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU2434852.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1016..1285
                     /locus_tag="N7C74_005215"
     CDS             1016..1285
                     /locus_tag="N7C74_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGU2434853.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          378 a          271 c          338 g          386 t
ORIGIN      
        1 ccgggtctgg tgctgattac ttcagccaaa aggaacacct gtatatgaag tgtatattat
       61 ttaaatgggt actgtgcctg ttactgggtt tttcttcggt atcctattcc cgggagttta
      121 cgatagactt ttcgacccaa caaagttatg tctcttcgtt aaatagtata cgaacagaga
      181 tatcgacccc tcttgaacat atatctcagg ggaccacatc ggtgtctgtt attaaccaca
      241 ccccaccggg cagttatttt gctgtggata tacgagggct tgatgtctat caggcgcgtt
      301 ttgaccatct tcgtctgatt attgagcaaa ataatttata tgtggccggg ttcgttaata
      361 cggcaacaaa tactttctac cgtttttcag attttacaca tatatcagtg cccggtgtga
      421 caacggtttc catgacaacg gacagcagtt ataccactct gcaacgtgtc gcagcgctgg
      481 aacgttccgg aatgcaaatc agtcgtcact cactggtttc atcatatctg gcgttaatgg
      541 agttcagtgg taatacaatg accagagatg catccagagc agttctgcgt tttgtcactg
      601 tcacagcaga agccttacgc ttcaggcaga tacagagaga atttcgtcag gcactgtctg
      661 aaactgctcc tgtgtatacg atgacgccgg gagacgtgga cctcactctg aactgggggc
      721 gaatcagcaa tgtgcttccg gagtatcggg gagaggatgg tgtcagagtg gggagaatat
      781 cctttaataa tatatcagcg atactgggga ctgtggccgt tatactgaat tgccatcatc
      841 agggggcgcg ttctgttcgc gccgtgaatg aagagagtca accagaatgt cagataactg
      901 gcgacaggcc tgttataaaa ataaacaata cattatggga aagtaataca gctgcagcgt
      961 ttctgaacag aaagtcacag tttttatata caacgggtaa ataaaggagt taagcatgaa
     1021 gaagatgttt atggcggttt tatttgcatt agcttctgtt aatgcaatgg cggcggattg
     1081 tgctaaaggt aaaattgagt tttccaagta taatgaggat gacacattta cagtgaaggt
     1141 tgacgggaaa gaatactgga ccagtcgctg gaatctgcaa ccgttactgc aaagtgctca
     1201 gttgacagga atgactgtca caatcaaatc cagtacctgt gaatcaggct ccggatttgc
     1261 tgaagtgcag tttaataatg actgaggcat aacctgattc gtggtatgtg ggtaacaagt
     1321 gtaatctgtg tcacaattca gtcagttgac agttgcctgt cagactgagc att
//
LOCUS       DBFIFM010000568         1373 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.guided.421, whole genome shotgun sequence.
ACCESSION   DBFIFM010000568 DBFIFM010000000
VERSION     DBFIFM010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.guided.421"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            45..1004
                     /locus_tag="N7C74_005216"
     CDS             45..1004
                     /locus_tag="N7C74_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU2434854.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1016..1285
                     /locus_tag="N7C74_005217"
     CDS             1016..1285
                     /locus_tag="N7C74_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGU2434855.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          377 a          271 c          338 g          387 t
ORIGIN      
        1 ccgggtctgg tgctgattac ttcagccaaa aggaacacct gtatatgaag tgtatattat
       61 ttaaatgggt actgtgcctg ttactgggtt tttcttcggt atcctattcc cgggagttta
      121 cgatagactt ttcgacccaa caaagttatg tctcttcgtt aaatagtata cgaacagaga
      181 tatcgacccc tcttgaacat atatctcagg ggaccacatc ggtgtctgtt attaaccaca
      241 ccccaccggg cagttatttt gctgtggata tacgagggct tgatgtctat caggcgcgtt
      301 ttgaccatct tcgtctgatt attgagcaaa ataatttata tgtggccggg ttcgttaata
      361 cggcaacaaa tactttctac cgtttttcag attttacaca tatatcagtg cccggtgtga
      421 caacggtttc catgacaacg gacagcagtt ataccactct gcaacgtgtc gcagcgctgg
      481 aacgttccgg aatgcaaatc agtcgtcact cactggtttc atcatatctg gcgttaatgg
      541 agttcagtgg taatacaatg accagagatg catccagagc agttctgcgt tttgtcactg
      601 tcacagcaga agccttacgc ttcaggcaga tacagagaga atttcgtcag gcactgtctg
      661 aaactgctcc tgtgtatacg atgacgccgg gagacgtgga cctcactctg aactgggggc
      721 gaatcagcaa tgtgcttccg gagtatcggg gagaggatgg tgtcagagtg gggagaatat
      781 cctttaataa tatatcggcg atactgggca ctgtggccgt tatactgaat tgtcatcatc
      841 agggggcgcg ttctgttcgc gccgtgaatg aagagagtca accagaatgt cagataactg
      901 gcgacaggcc tgttataaaa ataaacaata cattatggga aagtaataca gctgcagcgt
      961 ttctgaacag aaagtcacag tttttatata caacgggtaa ataaaggagt taagcatgaa
     1021 gaagatgttt atggcggttt tatttgcatt agcttctgtt aatgcaatgg cggcggattg
     1081 tgctaaaggt aaaattgagt tttccaagta taatgaggat gacacattta cagtgaaggt
     1141 tgacgggaaa gaatactgga ccagtcgctg gaatctgcaa ccgttactgc aaagtgctca
     1201 gttgacagga atgactgtca caatcaaatc cagtacctgt gaatcaggct ccggatttgc
     1261 tgaagtgcag tttaataatg actgaggcat aacctgattc gtggtatgtg ggtaacaagt
     1321 gtaatctgtg tcacaattca gtcagttgac agttgcctgt cagactgagc att
//
LOCUS       DBFIFM010000569         1005 bp    DNA     linear   BCT 12-FEB-2025
DEFINITION  TPA_asm: Escherichia coli strain 98_161798
            SAMD00495970-rid25093843.guided.475, whole genome shotgun sequence.
ACCESSION   DBFIFM010000569 DBFIFM010000000
VERSION     DBFIFM010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00495970
            Sequence Read Archive: DRR381706
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001424414.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001424414.2
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/05/2025 21:17:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 96
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 89 of 230
            Pseudo Genes (incomplete)         :: 124 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 33 of 230
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00495970-rid25093843.guided.475"
                     /strain="98_161798"
                     /serotype="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Japan"
                     /collection_date="2016-08-08"
     gene            complement(<1..286)
                     /locus_tag="N7C74_005218"
     CDS             complement(<1..286)
                     /locus_tag="N7C74_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="catalyzes the hydrolysis of Xaa-His dipeptides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HGU2434856.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDP"
     gene            547..1005
                     /gene="gpt"
                     /locus_tag="N7C74_005219"
     CDS             547..1005
                     /gene="gpt"
                     /locus_tag="N7C74_005219"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGU2434857.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWVMGVVFVPPISGR"
BASE COUNT          254 a          234 c          256 g          261 t
ORIGIN      
        1 taggatcttt cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc atatcgaggt
       61 gggcctgcaa gacgaccggt ttacgatttt ccatacctgc ggtagcaggt ttacgaatca
      121 ggatattacc tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa
      181 tgtattcagc gagttgctct tcatgatagg acgggtgagg aatagaacag attttggcaa
      241 aaatatccca cagcggctgt ggagataatt gagacagttc agacacgtta agtctccttg
      301 tcgatcaccc gcaaaacagt attgcaggtc acagggttag cagaaaatgt tgtcaacaca
      361 agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt
      421 gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat
      481 ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca
      541 cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa
      601 ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc
      661 ggtctggtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt
      721 tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa
      781 ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt
      841 gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tctttgcaaa accggctggt
      901 cgtccgctgg ttgatgatta tgttgttgat atcccgcaag atacctggat cgaacagccg
      961 tgggttatgg gcgtcgtatt cgtcccgcca atctccggtc gctaa
//
